LOCUS       DASWBF010000001         4913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_197347,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000001 DASWBF010000000
VERSION     DASWBF010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197347"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VF874_00005"
     CDS             complement(<1..1296)
                     /locus_tag="VF874_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9174531.1"
                     /translation="MPYFGTVLNRAARVMATGHGGQNLLADSTAVLLSGGDLMDLGSR
                     RLRDVPTPVGVFQVRAPGLATDFPPLRALDTSPGNLRHATSSLIGRESELDEVQAAVK
                     AHRLVTLTGVGGVGKTRLALEVAARLVDEFPHGVWFFELAAVTDPASVPDAVAAVLGI
                     TQQPGMSVSESIAATLEGRMRLLVFDNCEHVRDAAADLVESILAQSATVRILATSREG
                     LGIIPDEQLWLVPSLDLGAGIDSAAVDLFVERARSAASGFSMATTDEASAVVEICRRL
                     DGIPLAIELAASRMASMTASEVRDRLDQRFRLLVGFRRGLSRHQTLRHAVAWSYDLLD
                     EAQKSLLERCSVFAGGFDLESACAVTGSGDEFAILDLLDALVRKSLLVAERSSGRTRF
                     SMLETIRQFAEQQLVACGEASEIRAAHSRYFAGREADIMA"
     gene            1540..2349
                     /locus_tag="VF874_00010"
     CDS             1540..2349
                     /locus_tag="VF874_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9174532.1"
                     /translation="MSDTQIQAQHSTGLSRPNIERALFAAGKDLDRLEAADLAMLEDF
                     HTMGRIATSQLVDLAGITSEDEVLDAGSGVGGTARYVADRCGCAVTAVDLTEEYCDAR
                     WLNRLVGLDDQITVRRADVTDMPFADGTFRVVLSQHVQMNVADKAALYREARRVLAGG
                     GVLAIWDITSGEGGELDFPLPWADEPAVSHLVTSEALRAAVESSGFAIEQWNDLTDQA
                     SALMQTILTLPPSPLGLHAFVPDFAQKAANLTAALTDGRLRAIQGLARAID"
     gene            complement(2346..3395)
                     /gene="ruvB"
                     /locus_tag="VF874_00015"
     CDS             complement(2346..3395)
                     /gene="ruvB"
                     /locus_tag="VF874_00015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX9174533.1"
                     /translation="MSRFESTDEEDREVSAALTVGEGDIDASLRPRSLDEFIGQPRVR
                     EQLQLVIEGAKNRGGTPDHILLSGPPGLGKTSLAMIIAHELGSSLRLTSGPALERAGD
                     LAAMLSNLVEHDVLFIDEIHRIARPAEEMLYLAMEDFRVDVVVGKGPGATSIPIEVAP
                     FTLVGATTRSGALTGPLRDRFGFTAHMDFYEPAELEQVLRRSSGILGIELCADAGAEI
                     GRRSRGTPRIANRLLRRVRDYAEVRADGVITRDVAKAALQVYDVDELGLDRLDGAVLT
                     ALIRSFGGGPVGVSTLAVAVGEEATTVEEVCEPFLVRAGMIARTPRGRVATALAWTHL
                     GISPPSGLGQAGLFV"
     gene            complement(3392..3979)
                     /gene="ruvA"
                     /locus_tag="VF874_00020"
     CDS             complement(3392..3979)
                     /gene="ruvA"
                     /locus_tag="VF874_00020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9174534.1"
                     /translation="MIASIRGEVLEVALDHVVIEACGVGYRVNATPSTLATLRRGTEA
                     TLVTAMIVREDSMALYGFADSDTRDLFQTMLSVSGVGPRLAMATLAVHEAPALRQVLA
                     DGDVTALTRVPGIGKRGAERMVLELRDKVGAVAAAARAVNGHAVRGPVVEALVGLGFA
                     VKQAEEASDRVLAGDDAATTSSALRAALSLLGKSK"
     gene            complement(3976..4521)
                     /gene="ruvC"
                     /locus_tag="VF874_00025"
     CDS             complement(3976..4521)
                     /gene="ruvC"
                     /locus_tag="VF874_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9174535.1"
                     /translation="MRVMGVDPGLTRCGLSLIESVGRHVVALDVDVVRTPTDMPLHRR
                     LLAISDTAEHWMDTHQPDVIAIERVFSQQNVSTVMGTAQAGGVIALAAARRGIGVHFH
                     TPSEVKAAVTGSGVAGKAQVTAMVTKIFALQAKPTPADAADALAICHCWRAPTMARMA
                     QAEALVAQQRKKYEAKVKAAR"
BASE COUNT          819 a         1733 c         1574 g          787 t
ORIGIN      
        1 ggccatgatg tcggcttcgc gtccggcgaa gtagcgcgag tgggcggccc gaatctcgga
       61 tgcttcgccg caggcgacga gttgttgctc ggcgaactgg cggatcgtct ccagcatcga
      121 aaaccgggtc cgccccgaag acctctcagc gaccagcagc gacttgcgca ccagcgcgtc
      181 gagcagatcc aagatggcga actcatcacc ggaacccgtg acggcgcagg cactttcgag
      241 gtcgaatccc ccggcaaaca ccgaacaccg ctccagcagc gatttctgcg cctcatcgag
      301 caggtcgtag gaccacgcca ccgcgtggcg cagtgtctgg tggcgtgaca gcccacgcct
      361 gaaaccgacc agcagccgga accgctgatc gagacgatca cgcacctcgc tggcggtcat
      421 cgacgccatc cgcgaggccg ccaactcgat ggccagcggg atcccgtcta ggcggcggca
      481 gatctccacc accgcgctcg cctcatcagt ggtggccatc gagaacccgg aagcggcgct
      541 gcgggcgcgc tcgacgaaca aatccactgc ggcactgtcg atgcccgcgc caaggtccag
      601 cgagggcacc agccacagtt gctcgtcggg gattatcccc aacccttcgc ggctggtggc
      661 caggatcctc accgtcgccg actgcgcgag gatcgactcg accaggtcgg ctgcggcgtc
      721 gcgcacgtgc tcgcaattgt cgaacaccaa tagccgcatc ctgccctcca acgtggcggc
      781 gatgctctcg ctgacactca ttccgggttg ttgggtaatc cccaggactg cggccactgc
      841 atcgggcacc gacgccggat cggtaaccgc agccagttcg aaaaaccaga caccgtgagg
      901 aaattcatcg acgagacgtg ccgcgacttc caacgccagc cgggtcttgc cgactccgcc
      961 gacaccggtc aacgtcacga gccgatgagc cttgaccgcc gcttgcacct cgtcgagttc
     1021 ggactcgcgt ccgatcaagc tgctcgtcgc atgccggaga tttcccggac tcgtatcgag
     1081 cgcccgcaac ggcggaaaat ccgtcgccaa acccggcgca cgcacttgga agaccccgac
     1141 cggcgtcggc acgtcccgca accgtctgga tcccagatcc atcagatcac cgccgctgag
     1201 cagaactgcc gtcgagtcgg ccaacaggtt ctgaccacca tgtccggtgg ccatcacccg
     1261 tgccgcacga ttgagcaccg tgccgaagta gggcattgtc aagttgagcg aagtgggcgc
     1321 tgtcgccaat caaatcagcc tcatgcgtca catatgacct gggggttcac gagaacggat
     1381 cgcctcaaat cctggtctga ttgctcaact tgacaatgcc cgcacacccc cggtatttcg
     1441 gcgaatccgc ggtctggcgc agcgagcacc ccgccccggc cggccgcgcc gcgcttcggt
     1501 tcggagcagc cgattgcgac ccacccccaa ggagcagaca tgtcagacac ccagattcag
     1561 gcgcagcact ccactgggct atctcggccc aacatcgagc gggccctctt tgcggccgga
     1621 aaggatctcg accggctcga ggcagccgac ctcgcgatgc tcgaggactt ccacacgatg
     1681 ggtcgcatcg ccaccagcca actggtggac ctcgccggga tcaccagcga ggacgaggtg
     1741 ctcgacgccg gcagcggggt cggcggtact gcgcgctacg tcgccgaccg gtgcggctgc
     1801 gcggtgacgg cggtcgacct gaccgaggag tattgcgacg cccgttggct caatcgcctt
     1861 gtcggacttg acgatcagat cacggtccgc cgggcggacg tcactgacat gcccttcgcc
     1921 gatggtacgt ttcgggtcgt gctcagccag cacgtccaga tgaacgtggc cgacaaggcc
     1981 gccctgtacc gggaggcccg gcgggtgttg gccggcgggg gcgtgctggc aatctgggac
     2041 atcaccagtg gcgagggtgg cgaacttgac tttccgctgc cgtgggccga cgaacccgcc
     2101 gtcagtcatc tggtcacgtc cgaggcgttg cgcgctgctg tcgagtcatc ggggttcgcg
     2161 atcgagcagt ggaacgatct caccgatcag gcgagcgcgc tgatgcagac gatcctcacg
     2221 ctgcctccca gtccgctcgg gctgcacgcc ttcgtccccg acttcgcaca gaaggccgcg
     2281 aacctgacgg ccgccctcac cgacgggcgg ctgcgagcca tccagggcct ggcgcgcgca
     2341 atcgactaga cgaacaaacc ggcctggccc aaaccgctgg gcggcgaaat gcccaggtgc
     2401 gtccaggcta acgctgtcgc gacccgcccc cgtggtgttc gcgcgatcat cccggcccgc
     2461 accaaaaacg gctcgcacac ctcctcgacc gtcgtggcct cctcgcccac ggcgaccgcc
     2521 agcgtcgaca cgccgaccgg cccgccgccg aagctgcgga tcagcgccgt cagcaccgcg
     2581 ccgtccagcc tgtccagccc gagttcgtcg acgtcgtaaa cctgcagcgc ggccttggcg
     2641 acatcgcggg tgatgacgcc gtcggcccgc acctccgcgt agtcacggac ccgccgcaga
     2701 agccggttgg cgattcgcgg cgtgccccgc gatcgccgtc caatctcggc gcccgcgtcg
     2761 gcgcacaact cgatgccgag gattcccgac gaccgcctca gcacctgctc gagttcggcc
     2821 ggctcgtaga aatccatgtg cgcggtgaag ccgaaccggt cgcgcagcgg gccagtgagc
     2881 gcgccagagc gggtagtggc gccgacgagc gtgaacggcg ccacctcgat cggaatcgac
     2941 gtcgccccag gccctttccc gacgacgacg tcgacccgga aatcctccat cgccaggtac
     3001 agcatctcct cggcgggccg cgcgatgcgg tggatctcgt cgatgaacag cacgtcgtgc
     3061 tcgaccaggt tcgacagcat cgcggccagg tcacccgcgc gctccagcgc gggacccgac
     3121 gtcagcctca aggagctgcc gagctcgtgc gcgatgatca tcgccagcga tgtcttgccc
     3181 aacccgggtg gccccgacag caggatgtga tcgggcgtgc cgccgcggtt cttggcgccc
     3241 tcgatgacca gctgcagctg ctcgcgcacc cgcggctggc cgatgaactc gtccagcgag
     3301 cgcgggcgca ggctggcgtc gatgtcgccc tcgccgacgg tgagcgccgc ggaaacttcc
     3361 cggtcctctt cgtcggtcga ctcgaaccgg ctcatttgct cttgcccagc agcgatagcg
     3421 cggcccgcag cgcgctggac gtggtggctg cgtcatcgcc ggccagcacc cggtcgctgg
     3481 cctcctcggc ttgcttgacc gcaaagccaa ggcccacaag ggcttccacc acggggccgc
     3541 gcaccgcgtg gccgttgacc gcgcgcgccg cggccgccac cgccccgact ttgtcgcgca
     3601 gttccagcac catccgttcg gcgccacgct tgccgatgcc gggtacccgg gtcagcgcgg
     3661 tgacgtcacc gtcggcgagc acctggcgca gcgcgggcgc ctcgtgaacg gccagcgtcg
     3721 ccatcgcgag ccgcggcccg acacccgaca ccgacagcat tgtctggaac agatcgcggg
     3781 tgtcggaatc ggcgaatcca tagagcgcca tcgagtcctc gcgcacgatc atcgcggtga
     3841 cgagcgtggc ttcggtgccg cggcgcaggg tcgcgagcgt cgatggcgta gcgttgaccc
     3901 gatagccgac tccacacgcc tcgatgacga cgtggtcaag ggccacctcg aggacttccc
     3961 cgcgaatcga cgcgatcatc tcgccgcctt taccttggct tcatacttct tgcgttgctg
     4021 ggcgaccagc gcctcggcct gcgccatccg ggccatggta ggcgcccgcc agcagtgaca
     4081 gatcgccagc gcgtctgccg cgtctgcggg cgttggttta gcttgcagcg caaagatttt
     4141 ggtgaccata gcagtgactt gggctttgcc cgcaacgccg ctgccggtga cggccgcctt
     4201 gacctcgctt ggcgtgtgga agtgcacgcc gatgccgcgg cgggcagcgg caagcgcgat
     4261 cactcccccg gcctgcgccg tccccatcac ggtcgacacg ttctgttggg agaacacccg
     4321 ctcgattgcg atgacgtcgg gctgatgcgt gtccatccag tgctcggcgg tgtcgctgat
     4381 cgcgagcagc cgccggtgca gcggcatgtc ggtgggcgtg cgcaccacgt cgacgtcgag
     4441 ggccaccacg tgccggccga cgctctcgat cagcgacagc ccacagcgcg tcagccccgg
     4501 gtcgactccc atgacacgca cgcggcctcc gtcatctacg aacaattgtt cgatagaacc
     4561 taacgggttg acccgacatc aacgcgcaac gacacgccca ctcactgcgg cgccgacgcg
     4621 ggccgttggg ccgcgccggg ggtgcggaag cgtttcatat ggcgcgttcg cgttgccggg
     4681 tcggttgatg cgagttctcg gcccggttga gttctcaacc gatgtcaagt gttgcgacga
     4741 aagcggtggg cggttcatgc tggttgcgga acgggcgatg ccttggagcg cgtggcgact
     4801 cctgtcttgg cacgctcgtt ccgctcgttg cgtcgtgggt gtctgtgggg ggtgaccttg
     4861 cgccgggcgg cacggacctc gggtggcacg gtgtagcgtt cggcgacgat ggc
//
LOCUS       DASWBF010000002         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_855195,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000002 DASWBF010000000
VERSION     DASWBF010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855195"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VF874_00030"
     CDS             <1..209
                     /locus_tag="VF874_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9174536.1"
                     /translation="VDVADIDQLPAAVKAALDVDGPSVISLECAADEVPPFAPFLTKA
                     VTGTPGTNHLPNTKEHHSHVTINA"
     gene            190..857
                     /locus_tag="VF874_00035"
                     /pseudo
     CDS             190..857
                     /locus_tag="VF874_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     gene            863..1882
                     /locus_tag="VF874_00040"
     CDS             863..1882
                     /locus_tag="VF874_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HEX9174537.1"
                     /translation="MSTPAVSLIDVSSYLPGAPIGTDYYSAFAGADELRDNLMFRAPK
                     FRHHVGPDETAIDMVERATAGLIDRHGQDTIANVDVLITHSQMPDMPFYGGGGGMAHR
                     LGMHPKWVLDLHNGGCAAFVLGMNVARQLLASGEGRTALIAVAQNAAGQIFDQEGIRR
                     KAQASVPGDGAAVGLVAVSEQSPILDVECRTYGEYAGDMTIAVDPPRKWWQPGPGEGY
                     IGFTETKITKVLARGNRQVPEVALAVCDRIGMQSKDLDLLVTNQPNRVFLRNWREALE
                     MPRERHRDTFDQCGNLFGAGIPINLDRAICDGQLKAGDTVMMAAFAHAGDFAGAAAIR
                     WGGRN"
     gene            1882..2837
                     /locus_tag="VF874_00045"
                     /pseudo
     CDS             1882..2837
                     /locus_tag="VF874_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736908.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
BASE COUNT          506 a          978 c          895 g          458 t
ORIGIN      
        1 cagtggacgt cgccgacatc gaccagctgc ccgccgccgt aaaggccgcg ctggacgtcg
       61 acggcccgtc ggtcatcagc ctcgaatgcg ccgccgacga agttcccccg ttcgcaccgt
      121 ttctcaccaa ggccgtcacc ggaacgcctg gcacaaacca tcttccgaac accaaggagc
      181 accattccca tgtcactatc aacgcttgac gatattgcta cccacaccgg cagcaccgac
      241 ccgatcgacg gagtaatccg catcgagaat gctccccgcg agaaggccac cccgatcatc
      301 ttggagatga tgcggtcggt gtacccgcac gaggaggtgt tttcggcccc tacgtcacgg
      361 tgaatgacta catcgactgc ccgcccgacg agttgttcga gtacctggcc gacacccgca
      421 gcctggagga gtggacctat agcctgcgcg gtttcacacc gaccgaggaa cccggcctgt
      481 ggctggcata tgaccggctg ggttcgcaga ccgagattta cacccggacg gtggcaaacg
      541 aggcggctcg taccgtcgac tatcactgcg cctgggatca gggcaagcac ctgtggatga
      601 tctacctgat gcgggtcatc gacgcgcagc tggtgctcga caagcccggt tcggtggtgc
      661 tgtggaccaa ttgccaccac ccgttctacg accacaaccc ctacccgcag accgcgccgc
      721 cgcaacgccc ggtgtgggtg ggtgacttct gggacatgtt cggcgccggg catctgctgg
      781 agttgaaaaa cctgaaggcc atcgccgaat accgccacca caacggcctg ccggtgacac
      841 cggtctggat gagatgagca ccatgagcac acccgccgtc agcctgatcg atgtctccag
      901 ctatctgccc ggcgcgccga tcggcacgga ttactactcg gcgttcgccg gagccgacga
      961 gttgcgcgac aacttgatgt tccgcgcgcc gaagttccgc catcacgtcg ggcccgacga
     1021 gaccgcgatc gacatggtcg agcgggccac cgccgggctg atcgaccggc acggccagga
     1081 cacgatcgct aacgtcgacg tgctgatcac ccactcccag atgcccgaca tgccgttcta
     1141 tggcggtggc ggtgggatgg cccaccggct cggcatgcac ccgaagtggg tgctcgacct
     1201 gcacaacggc ggatgcgccg cgtttgtgct ggggatgaat gtggcccgcc agctactcgc
     1261 gtccggcgaa ggacgcaccg cgctgatcgc ggtggcgcag aacgccgccg gacagatctt
     1321 cgatcaggag gggatccgcc gcaaggcgca ggcaagcgtt cccggtgacg gggcggccgt
     1381 cggcctggtg gccgtgtccg agcaatctcc catcctcgac gtcgagtgcc gcacctacgg
     1441 cgagtacgcc ggcgacatga ccatcgccgt tgacccgccg cgcaagtggt ggcaacccgg
     1501 gcccggcgag ggctacatcg gtttcaccga gaccaagatc accaaggtgc tcgcccgcgg
     1561 caaccgtcag gtgcccgagg tggcgttggc ggtgtgtgac cgaattggca tgcagtccaa
     1621 ggaccttgac ctgctggtca ccaaccagcc caaccgggtg ttcctgcgca actggcgcga
     1681 agcgctcgag atgccgcgcg aacgccaccg tgacacattc gatcagtgcg gtaatctgtt
     1741 cggggccggc atcccgatca acctggatcg ggcgatttgc gacggccagc tcaaggccgg
     1801 cgacaccgtg atgatggccg cctttgccca cgccggcgac ttcgccggcg ccgcagcgat
     1861 ccgctggggt ggtcgcaact gatgacgtcg gtcagctatg ccacccccat cgccgcggag
     1921 atcctggcga cgttgcccga tgcggtcgat ccactggcgc tctcgctcaa cgagaacccg
     1981 ttcgcgccgc tgcccgcagt gcgcgcggca ctgatcgcgt cgatcgacgc ggccaaccgc
     2041 tacccgcaat tcctacccga gcagctgcgc ggcctgatcg ccggccgcat cggggtgcgc
     2101 gaccagcagg tcatcctggg cgcgggtgcg accggcgtgg tgctgcaggt gctgcacgcg
     2161 gtgaccagcc cccggcgatc gaatcgtgat gacctccccg acattcgacg ggtatccgat
     2221 cttcgcgcag atggcccggt tgatctcggt gacggtgccg ctggacgaac acggaaacca
     2281 tgacctggac gcgatggccg acgccgcgtg caacgccaag gtggtggtgc tatgccggcc
     2341 gcacaatccg accggcacgc tggagtccgc cgccgacgtg gagcggttcc tgcagcgagt
     2401 gccgccggac accgtggtgc tactggacga ggcctacatc gagttcgtcg cccccgagca
     2461 ccgcctcgac gcgcccgctt tgatccagcg gttcccgaac gtggtggtgc tgcgcacgtt
     2521 ctcgaaggcc tacgggttgg ccgggctgcg gatcggctac ggcttcgggg cgcccgaact
     2581 cgccaagacg ctgtggacga tgcagctgcc gttcgggatg agtatcacca gcgcggtcgc
     2641 ggtcgccgcc tcctatgatg cccaagatca actatggcaa cgcatccggc tgatcaccag
     2701 cgagtgccgc tacctgcaac gccggatacg ggcgatgggc atctacaccc ccaccgcgca
     2761 cgccaacttc atctacctgc ccgagcaggg ccgcgcctgg cgacacgcat tcaacaacgc
     2821 gggcctacac gtgcgtc
//
LOCUS       DASWBF010000003         5062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_394915,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000003 DASWBF010000000
VERSION     DASWBF010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394915"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="VF874_00050"
     CDS             complement(<1..1722)
                     /locus_tag="VF874_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX9174538.1"
                     /translation="MAPTRGMSTAPARHDPDRLLAGYRAARTQEALFELRGRPKAGYD
                     EFVDEAGHIRPAWTELADAVGERGRAGLDQLRSIVSGLVDNDGITYIQIDHSVDRAGD
                     AVTNGHGAVSPGPWRLDGLPLLISAADWEVLEAGLVQRSRLLDAVLTDLYGPRRSILS
                     GLLPPQLLFGHPGYIRAARGIDIPGRHQLFMHGCDVGRDASGTFRVNADWTQAPSGAG
                     YALADRRVVSHAMPDLYERIGPRPTSPFAQALRLALIDAAPDAADDPVVVVLSPGIHS
                     ETAFDQAYLATVLGFPLVESADLVVRDGKLWMRSLGTLKRVDVVLRRVDADYADPLDL
                     RADSRLGVVGLVEVQRRGACTVVNTLGSGILESPGLLRFLPVLAERLLGETPLLHTAP
                     VFWGGIDAERSHLLAHLPSLLIKSTIGGDTIVGPALSARQRASLAARIEATPWQWVAQ
                     ELPQFSSAPTGYRASTLASAGVGLRLFTVSQRGGYAPMIGGLGYVMAPGNAAYTLNTV
                     AAKDVWVRPTERTRAETLTVPSVDLPTMTGAGTQGVSSPRVLSDLFWMGRYGERAENL
                     ARLLTVTR"
     gene            complement(1722..>5062)
                     /locus_tag="VF874_00055"
     CDS             complement(1722..>5062)
                     /locus_tag="VF874_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9174539.1"
                     /translation="DRLVEVYPHVVRLRPAPHSRTPIEAYSLTVEPADHFINWQQDAF
                     GNFLARLVFPNRTRQLTITVGLIADLKVINPFDFFIEDWAETVPFALRPSGSSTPFAL
                     RPSGPPQAGGAPTSTPFDYPKAMAEDLQPYLRPVDEDDDGSGPGELVREWVSRFQVPK
                     GIRTIDFLVALNRAVNADVAYSVRMETGVQAPDVTLRNAIGSCRDSAWLLVSILRQLG
                     LAARFVSGYLVQLTSDVEALDGPSGPAADFTDLHAWTEVYIPGAGWIGMDPTSGLFAG
                     EGHIPLAATPHPSSAAPIDGATEMCDTVLDFSNTVTRIHEDPRVTLPYTDAAWATICD
                     VGAEVDERLAAGDVRLTVGGEPTFVSIDNQVDDEWTVEADGPHKRQRAADLAARLKKV
                     WAPNGLVQWGQGKWYPGEPLPRWQIALHWRADGQPLWRDEALLADPWSDDDRDMDPGA
                     ARRVLEEIAVGLGLPASQVRPAYDDPLSRLAAKVRLPAGDAVVPEDDLEDDSPGARAA
                     LLDRLDESVVDPAAYVLPLHRRDDDTGWASADWKLRRGRIVLLDGDSPAGLRLPLDAI
                     SWRPPRPSFEADPLARRPGLADAESADATVEDDDTLPVTATVAEVRDGLPYVFLPPTE
                     ELELFVDLAGRVEAAGAKTGCPLVIEGYGPPSDPRLRSATITPDPGVIEVNVAPTAGF
                     VEQRQQLETLYEQARLARLSTESFDVDGTHGGNHITLGGITPRDSPLLRRPDLLVSML
                     TYWQRHPSLSYLFAGRFVGTTSQAPRVDEGRAEALYELEIAFAEIARLTRRGRARPWV
                     TDRALRHLLIDITGNTHRAEFCIDKLYSPDSARGRLGLLELRGFEMPPHFQMAMVQSL
                     LVRSLVAWFWDEPVRAPLIRYGANLHGRYLLPHFIIHDIAEVAADLRAQGIDFETAWL
                     DPFTEFRFPRIGTAVFDGVEIELRGAIEPWNTLGEEATAGGTARYVDSSVERIQVRII
                     GADRQRHVVTANGYPVPLLATDNPDIQVAGVRFRAWQPPSALHPTITVDGPLRFELVD
                     AATGMSRGGCTYHVSHPGGRAYDEPPVNAVEAESRRSRRFEATGFTPSPVDIAGIREK
                     QARLSTDVGAPGILDLRRVRTVLR"
BASE COUNT          762 a         1750 c         1820 g          730 t
ORIGIN      
        1 acgcgtgacg gtcagaagcc gcgcgaggtt ctcggcccgc tcgccgtagc gccccatcca
       61 gaacaggtcc gacagcacgc gcggcgagct gacaccctgc gttcccgcac cggtcatcgt
      121 cggcaggtcc accgacggca ccgtcaacgt ctcggcccgc gtgcgctccg tcggtcggac
      181 ccaaacatct ttggcggcaa cggtgttcaa tgtgtacgcg gcgttgcctg gcgccatcac
      241 gtagccgagg cccccgatca tcggggcgta gcctccgcgc tgcgacacgg tgaacagccg
      301 cagtcccacg cccgccgacg cgagcgtgct ggcgcggtaa cccgttggcg ccgaggagaa
      361 ctggggcagt tcctgggcga cccactgcca cggtgtcgcc tcgatacgcg cggccaggct
      421 ggcccgctgg cgcgccgaca gcgcgggccc gacgatcgtg tccccgccga tggtggactt
      481 gatcaacagc gacggcagat gcgccagaag gtgcgaacgt tccgcgtcga tgccgcccca
      541 gaacaccggc gcggtgtgca gcagcggcgt ctcccccagc agccgctcgg ccagcacagg
      601 caagaaccga agcagcccag ggctttccag gatgccgctg cccagcgtgt tgaccaccgt
      661 gcatgcgccg cgccgttgca cctcgaccag ccccaccacc cccaaccgcg aatcggcgcg
      721 taggtcaagg ggatcggcgt agtcggcgtc gacgcgccgc agcaccacgt cgacgcgttt
      781 gagcgtgccg agcgaacgca tccagagttt gccgtcgcgc acgacgaggt cggcgctctc
      841 gacgagcggg aagcccagca ccgtcgccag gtacgcctgg tcgaacgcgg tctcggagtg
      901 gatgccgggg ctgagcacca cgaccaccgg gtcgtcggcc gcgtcgggag ccgcgtcgat
      961 cagcgcgagc cgcagcgcct gtgcgaacgg cgacgtgggt cgcggtccga tccgctcgta
     1021 gaggtcgggc atcgcgtgcg agacgacccg gcggtcggcc agcgcatacc ccgcacccga
     1081 gggcgcctgg gtccagtccg cgttcacccg gaaggtgccc gacgcgtcgc ggccgacatc
     1141 gcagccgtgc atgaacaact ggtgccggcc cgggatgtcg atgccgcggg cggcccggat
     1201 gtagccggga tggccgaaca gcaattgcgg cggcaacagg ccggacagga tcgaccgccg
     1261 cggcccgtac aggtcggtca gcaccgcgtc cagcagccgc gaccgctgca ccagaccggc
     1321 ttccagcacc tcccagtcgg cggccgagat caacagcggc agcccgtcga gccgccacgg
     1381 gccgggtgac acggcaccgt gaccattcgt caccgcgtcg ccagcccgat cgacactgtg
     1441 gtcgatctgg atgtaggtga tcccgtcgtt gtcgacgagc ccgctgacga tggatcgcag
     1501 ctggtcaagc ccggcgcggc cgcgctcgcc gaccgcgtcg gccagctctg tccacgcggg
     1561 ccggatgtgg cccgcctcgt cgacgaactc gtcgtagccg gccttcggtc tcccccgcag
     1621 ctcgaacagc gcctcttggg tgcgcgcggc gcggtagccg gcgagcaggc ggtcagggtc
     1681 gtggcgggcc ggggccgtcg acatgccgcg ggtgggggcc attaccgaag aacggtacgc
     1741 acgcgtcgca ggtcgaggat gcccggcgcg ccgacgtcgg tggacaatcg cgcctgcttc
     1801 tcccggatgc cggcgatgtc gaccggtgac ggcgtgaagc cggtggcctc gaagcggcgg
     1861 ctgcgccgcg actcggcctc gacggcgttc accggcggct cgtcgtaggc gcggccgccg
     1921 gggtgcgaca cgtggtaggt gcagccgccg cgcgacatcc cggtcgcggc gtcgacgagc
     1981 tcgaagcgca gcggcccgtc gacggtgatc gtggggtgca gcgcgctggg cggctgccac
     2041 gcgcggaagc ggacgcccgc gacctggatg tcagggttgt cggtggccag cagcggcacc
     2101 gggtagccgt tggctgtgac gacgtggcgc tgccggtcag cgccgatgat gcggacctgg
     2161 atgcgttcga cggacgagtc gacgtaccgg gcggtgccgc ccgcggtggc ttcctcgccg
     2221 agcgtgttcc acggttcgat cgcaccacgc agttcgatct cgacgccgtc gaacaccgct
     2281 gtgccgatgc gcgggaaccg gaactcggtg aacgggtcca gccacgcggt ctcgaagtcg
     2341 atgccctgtg cccgtaggtc cgcggcgact tcggcgatat cgtgaatgat gaagtgcggc
     2401 aacaggtatc ggccgtgcag gttggcgccg taccggatca gcggggcgcg caccggctcg
     2461 tcccagaacc aggcgaccag tgagcgcacc agcagcgact gcaccatcgc catctggaaa
     2521 tggggcggca tctcgaatcc gcgcagctcc aagaggccca gccggccgcg tgcgctgtcg
     2581 gggctgtaga gcttgtcgat gcagaactcg gcgcggtggg tgttgccggt gatgtcgatg
     2641 aggaggtgcc gcagcgcacg gtcggtcacc cacggccgcg cgcggcctct gcgtgtcagt
     2701 cgggcgatct cggcgaatgc gatctcgagc tcgtaaagcg cctccgcgcg gccttcgtcg
     2761 acccgcggcg cctgcgacgt ggtgccgacg aaccggcccg cgaacaggta cgacagcgac
     2821 gggtgccgct gccagtacgt cagcatagag accagcaggt cggggcggcg cagcagcggc
     2881 gagtcgcgcg gcgtgatgcc gccgagcgtg atgtggttgc cgccgtgggt gccgtcgacg
     2941 tcgaacgact cggtggacag ccgcgccagc cgcgcctgct cgtacagcgt ctccagttgc
     3001 tgcctttgtt cgacgaatcc ggcggtcggc gcgacgttga cctcgatgac gccggggtcc
     3061 ggggtgatcg tcgccgaccg cagccgcggg tccgacggcg gcccgtagcc ctcgatgacc
     3121 agcgggcagc cggtcttcgc gcccgcggcc tcgacgcgac cggccagatc cacgaaaagc
     3181 tcgagctcct cagtgggcgg caggaacaca tacggcagcc cgtcgcggac ctcggcgacc
     3241 gtcgcggtga cgggcaacgt gtcgtcgtct tcgactgtgg cgtcggcgga ttcggcgtcg
     3301 gccaacccgg gccggcgcgc cagcgggtcg gcctcgaacg atggtcgcgg cgggcgccag
     3361 ctgatcgcgt ccagcggcaa gcgcagcccg gccggagaat cgccgtccag caacacaatt
     3421 cggccccggc gcagcttcca gtcggcactg gcccagccgg tgtcgtcgtc acggcggtgc
     3481 agcggaagca catacgcggc cgggtcgacg acggactcgt cgagcctgtc gagcagcgcc
     3541 gcgcgcgccc cgggactgtc gtcctccaga tcgtcctcgg gcaccaccgc atcacccgcc
     3601 ggcaggcgta ctttggcggc caatcgggag agcgggtcgt cgtaggcggg ccggacctgg
     3661 ctggcgggca ggccgagtcc gaccgcgatc tcttcgagca cccgccgcgc ggcgcctggg
     3721 tccatgtcgc ggtcgtcgtc tgaccacggg tcggccagca gcgcttcgtc gcgccacagc
     3781 ggctgtccgt cggcgcgcca gtgcagcgca atctgccagc gcggcaacgg ttctccggga
     3841 taccacttgc cctggcccca ttgcacgaga ccgttcggag cccacacctt cttcaaccgt
     3901 gccgcgaggt cggccgcgcg ctggcgtttg tgcggtccgt cggcttcgac ggtccactcg
     3961 tcgtcgacct ggttgtcgat cgacacgaac gtcggctctc cgccgaccgt caagcggacg
     4021 tctccggcgg cgagccgctc gtcgacctcg gcgccgacgt cgcagatcgt cgcccacgcc
     4081 gcgtcggtgt agggcagcgt gacgcgcggg tcctcgtgga tgcgggtgac cgtgttggag
     4141 aagtccagca cggtgtcgca catttcggtg gcgccgtcga tcggcgcggc cgacgacggg
     4201 tgcggggtgg ccgccagcgg aatgtggccc tcgccggcga acagtcccga cgtcgggtcc
     4261 attccgatcc aaccggcgcc ggggatgtac acctcggtcc aggcgtgcag gtcggtgaag
     4321 tcggccgccg gtccggacgg gccgtcgagg gcctcgacgt cggacgtcag ctgcaccaga
     4381 tagccggaga cgaaccgggc ggccaggccc agctgacgca ggatcgacac cagaagccag
     4441 gccgagtcgc ggcacgaccc gatcgcgttg cgcagcgtga cgtcgggtgc ctgcaccccc
     4501 gtctccatgc gcacgctgta ggcgacatcg gcgttgacgg cgcggttcag cgcgaccagg
     4561 aagtcaatcg tgcggattcc cttcggcacc tggaaccggc tcacccattc tcggaccagc
     4621 tccccagggc cggagccgtc gtcgtcctcg tcgaccggcc gcagatacgg ctgcaggtcc
     4681 tcggccatcg ctttggggta gtcgaatggc gtcgatgtgg gggcacctcc cgcttgcggg
     4741 ggaccactcg ggcgaagagc aaatggcgtc gatgagccac tcgggcgaag agcaaatggc
     4801 actgtttcgg cccagtcttc gatgaagaag tcgaatgggt tgatcacctt caagtcggcg
     4861 atcaggccga ccgtgatcgt cagctgccgg gtgcggttcg ggaaaaccag ccgggccagg
     4921 aagttgccga acgcgtcctg ctgccagttg atgaagtggt cggcgggctc gacggtcagc
     4981 gagtaggcct cgatcggggt gcgggagtgc ggcgcggggc gcagccgcac gacgtgcggg
     5041 tacacctcga ccagccggtc ga
//
LOCUS       DASWBF010000004         5151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_460761,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000004 DASWBF010000000
VERSION     DASWBF010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460761"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VF874_00060"
     CDS             <1..416
                     /locus_tag="VF874_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9174540.1"
                     /translation="RMKARWRDDLSRDGKPARAVDTRVARGQILYDAPELIIPVLVPD
                     GAHDYPDSARTAAEHTMFTVAVGAAVQAMLVALAVRGVGSCWVGSTIFAADEVRDELE
                     LPAQWEPLGAIAIGYPDEPLTPREPAPVAGLLVHR"
     gene            413..955
                     /locus_tag="VF874_00065"
     CDS             413..955
                     /locus_tag="VF874_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9174541.1"
                     /translation="MSLRDSVIATLRNWQPPDFAQDTLREAVLAFVVARPDACRRECE
                     PGHITASTLLFDDAGTHALLTLHPRLGRWVQLGGHCEDADTDIVAAARREAVEESGVA
                     DLSVQPELAAIHVHAVTCSLGVPTRHLDLQFVARAPADARIEVSDESLDLRWWDLAAM
                     PHDTDYGLAQLVSYAQRLCG"
     gene            complement(956..2068)
                     /locus_tag="VF874_00070"
     CDS             complement(956..2068)
                     /locus_tag="VF874_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX9174542.1"
                     /translation="MDPSRVDVPGGGAKRWGVDAVVLVGGQGTRLRPLTLSAPKPMLP
                     TAGLPFLTHLLSRIAAAGIEHVVLGTSYKPGVFESEFGDGSKLGLQIDYVTETEPLGT
                     GGAIANVKHKLRHDTTLVFNGDVLSGADLSALLDTHRQLDADVTLHLVRVGDPRPFGC
                     VPTDDDGLVTAFLEKTQDPPTDQINAGCYVFRRPLIDRIPTGRPVSVEREVFPALLSD
                     GLRVCGYVDATYWRDMGTPEDFVRGSADLVRGIAPSPALAGHSGEKLVHDGAAVAPGA
                     LLIGGTVVGRGAEIGPGARLDGAVIFDGARVEAGSVIERSIIGFGARIGPRALIRDGV
                     IGDGAHVGARCELLRGARVWPGVTIPDGGIRFSTDV"
     gene            complement(2080..2988)
                     /locus_tag="VF874_00075"
     CDS             complement(2080..2988)
                     /locus_tag="VF874_00075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9174543.1"
                     /translation="MSDELTVITVTYSPGLHLHRFLATLAQATDRPVSVVMADNGSTD
                     GSPEAALADHPNTRLLHTGANIGYGAAVNRAVAEMGPETDLFIVANPDVQWGPASIDL
                     LLEAAGRWPRAGTLGPLIRDPDGSVYPSARHLPSLIRGGLHAVVGPFWRNNPWTAEYR
                     QDRVHPSERPVGWLSGSCLLVRKAAFDEVHGFDERYFMYMEDVDLGDRMGRAGWQNVY
                     APAAEILHDKGHATGRDPGRNLAAHHASTYIYLADRHDRWWQAPLRWAMRAALAVRLS
                     LAVRRSAMSARREEQRARRELAKGLR"
     gene            complement(3028..3891)
                     /gene="rfbD"
                     /locus_tag="VF874_00080"
     CDS             complement(3028..3891)
                     /gene="rfbD"
                     /locus_tag="VF874_00080"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX9174544.1"
                     /translation="MGDRIVITGARGQVGRRLSAEAARLGYDVVALSSGEWDITDPAE
                     GEGIVEAGDMVVNCAAYTAVDAAEANPDAANAVNALGAGNVARTCARAGARLIHLSTD
                     YVFSGHFDDVPLPYDLDDRPEPLSVYGQSKLAGERAVHAELPDAQVVRTAWVYTGVGS
                     DFVAAIAGRAADDGPIDVVSDQIGSPTFVGDLVGALLQIVTGGITAALLHAANAGAVS
                     RFEQARAVFAALGADPQRIRPVTSDRHPRPAPRPSYSALSPRLSAAAGLTPLRPWRDA
                     LAAALREAGGS"
     gene            3996..>5151
                     /locus_tag="VF874_00085"
     CDS             3996..>5151
                     /locus_tag="VF874_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX9174545.1"
                     /translation="MPTAGPAASARRGVRVVCTLVAVAIVLATGLAWSKIKSLEDGIR
                     HISSPLLGTGGEDGAIDILLVGMDSRTDAHGNPLSEEELAQLHAGDDVSTNTDTIILV
                     RIPNNGKSATAISIPRDSYVQAPGLGKTKINGVYGQVKEDKRIQLVESGVSDAQAESE
                     STEAGREVLIKTVADLTGVTVDHYAEIGLLGFALITDAIGGVDVCLKDEVYEPLSGAD
                     FPAGWQRLDGPQALSFVRQRHELPRGDLDRVVRQQVVMASLAHQVISGKTLSSPSALN
                     SLQNAIQRSVVLSDGWDIMDFIKELEKLSAGNVAFATIPVLDESGWSDDGMQSVVRVD
                     QHQVQEWVGGLLEDQAQGKTEELAYTPAKTTANVVNDTDINGLAASVSEVL"
BASE COUNT          790 a         1806 c         1769 g          786 t
ORIGIN      
        1 accggatgaa ggcccggtgg cgcgacgacc tcagccgcga cggaaaaccg gcgcgcgccg
       61 tcgacacccg tgtcgcacgc ggtcaaatcc tttacgacgc gccggaactg atcattccgg
      121 ttctggtccc cgacggcgcg catgactatc ccgacagcgc tcgcaccgcg gccgagcaca
      181 cgatgttcac cgtcgcggtc ggtgccgccg tgcaggcgat gctggtcgcg ctggccgtgc
      241 gtggggtggg cagctgctgg gtgggctcga ctatcttcgc cgccgacgag gtccgcgacg
      301 aactcgagtt gcccgcacag tgggaaccgt tgggcgccat cgcaatcggg tatcccgacg
      361 agccgctcac cccacgcgag cccgcaccgg tggctgggct actggtgcac aggtgagtct
      421 ccgcgactcg gtgatcgcca cgctgcgaaa ttggcagcca ccggatttcg cgcaggacac
      481 gctgcgagaa gcggtcttgg cgttcgtggt cgcacgcccg gacgcgtgcc gacgcgagtg
      541 cgagccggga cacatcaccg cctcgacgct gctgttcgac gatgccggca cgcacgcgct
      601 gctcacgctt catccgcggc tgggtcgctg ggttcagctc ggcgggcact gtgaagacgc
      661 cgacaccgac atcgtcgccg cggcccggcg cgaggcggtc gaggagtccg gcgtcgcgga
      721 cctgtccgtc cagccggaac tggccgccat tcacgtccac gccgtcacct gctcgctcgg
      781 tgtgcccacc cgccacctcg accttcagtt cgtcgcccgc gccccggccg acgcgcggat
      841 cgaggtcagc gacgagtcac tggacctgcg ctggtgggac ctggccgcga tgcctcatga
      901 cacggattac ggcctggccc aactggtttc gtacgcgcaa cggctatgcg gctgatcaga
      961 cgtccgtcga gaaccggatg ccgccgtcgg gaatcgtgac gcctggccag acccgcgcgc
     1021 cgcgcagcag ttcgcagcgc gcgccgacgt gagcgccgtc gccgatcacc ccgtcgcgga
     1081 tcagcgcgcg cgggccgatc cgggcgccga acccgatgat cgaccgctcg atcaccgacc
     1141 cggcctccac ccgcgccccg tcgaagatca ccgcgccgtc cagccgcgcg cccgggccga
     1201 tctccgcacc tcggccgacc accgtgccgc cgatcagcag cgcgcccggc gccaccgcgg
     1261 cgccgtcgtg caccagcttc tccccgctgt ggccggcgag cgccggtgac ggcgcgatgc
     1321 cgcggacaag gtcggcggag ccgcggacga aatcttccgg ggtgcccatg tcgcgccagt
     1381 acgtggcgtc cacgtagccg cacacccgca gcccgtccga caacagcgcc gggaacacct
     1441 cgcgttccac cgacaccggg cgcccggtgg ggatgcggtc gatcaacggc cgccggaaca
     1501 cgtagcaccc ggcgttgatc tggtcagtcg gcggatcctg tgtcttctcc aggaacgcgg
     1561 tgaccagccc gtcgtcgtcg gtcggcacgc agccgaaggg ccgtgggtca ccgacccgca
     1621 ccaggtgaag tgtcacgtcg gcgtcgagtt ggcggtgggt gtcgagcagc gccgacaagt
     1681 ccgccccgga gagcacgtcg ccgttgaaca ccagcgtggt gtcgtgccgc agtttgtgct
     1741 tgacgttggc gatcgcgccg ccggtgccca gcggctcggt ctcggtgacg tagtcgatct
     1801 gcagcccgag cttgctgccg tcgccgaact ccgattcgaa gacgcccggc ttgtaggacg
     1861 tgccgagcac cacgtgctcg ataccggccg ccgcgatccg cgacagtagg tgcgtcagga
     1921 acggaagccc cgcggtcggc agcatcggct tgggcgccga gagcgtcagg gggcgcagcc
     1981 gggtgccttg cccgccgacg aggacgacgg cgtctacccc ccatcgcttc gctccacccc
     2041 ctggaacgtc gacccgcgac gggtcgatcg gagcgtctgt cacctcagtc ctttcgccag
     2101 ctcgcgccga gccctttgct cttcgcgacg agcgctcatc gccgagcggc gcaccgccag
     2161 cgataatcgc accgcgagcg cggcccgcat cgcccaccgc agcggcgcct gccaccaccg
     2221 atcgtgccgg tcggcaaggt aaatgtaggt gctcgcgtgg tgggccgcga ggttacggcc
     2281 aggatcgcgg ccggtggcat ggcccttgtc gtgcaagatc tccgccgccg gcgcgtagac
     2341 gttctgccag ccggcccggc ccatccggtc gccgaggtcg acgtcttcca tatacatgaa
     2401 gtaccgctcg tcgaacccgt gcacctcgtc gaatgcggcc ttgcgcacca acaggcacga
     2461 ccccgacagc cacccgaccg gccgctcgct gggatgcacc cggtcctgcc ggtactcggc
     2521 ggtccacggg ttgttgcgcc agaacgggcc gaccacggcg tgcaggccgc cgcggatcag
     2581 gcttggcagg tggcgcgccg acggatatac cgagccgtcc ggatcgcgga tcagcggccc
     2641 cagcgtgcca gcgcggggcc atcggccggc cgcctcgagt aacaggtcga tgctcgcagg
     2701 cccccactgc acgtcagggt tggcgacaat gaataagtct gtctccggcc ccatttcggc
     2761 gaccgcgcgg ttgacggccg cgccgtaccc gatgttggcg ccggtgtgca gcagccgggt
     2821 gttgggatgg tcagccagcg cggcctcggg gctgccgtcg gttgagccgt tgtcggccat
     2881 cacgacgctc actggccggt cggtggcttg cgccagcgtg gccaagaacc ggtgcaggtg
     2941 tagcccgggt gagtacgtca ccgttatcac ggtcagttcg tcactcacgg cgtagagggt
     3001 aacggttcgg cggtggcgcg accgtcgtca gcttccgccg gcctcacgca aggccgcggc
     3061 cagcgcgtcg cgccatggcc gcagtggcgt caacccggcc gccgccgaca gccgcggcga
     3121 cagcgcggag tagctcggcc gcggtgccgg gcggggatga cgatcgctgg tcaccggccg
     3181 aatccgctgc gggtcggcgc cgagggccgc gaacactgcc ctggcttgct cgaatcggct
     3241 gacggcgccg gcgttggcgg cgtgcagcag cgccgcggtg atcccgcccg tgacgatctg
     3301 cagcagtgcg ccgaccagat cgccgacgaa cgtgggtgat ccgatctggt cggataccac
     3361 gtcgatcggc ccgtcgtcgg cggcgcgccc cgcgatggcg gcgacgaagt cactgccgac
     3421 gcctgtgtag acccatgccg tgcggacaac ttgagcgtcg ggcagctcgg cgtgcaccgc
     3481 ccgctcgccg gcgagcttgc tctgtccgta caccgacagc ggctcggggc ggtcgtcgag
     3541 gtcgtacggg aggggcacgt cgtcgaagtg gccgctgaac acgtagtcgg tggacagatg
     3601 gatcagccgg gctccggcgc gggcgcaggt ccgcgcgacg ttccccgcgc ccagggcgtt
     3661 cactgcgttc gcggcgtccg gattcgcctc ggcggcgtcc acggccgtat aggccgcgca
     3721 gttgaccacc atgtcgcctg cttcgacgat tccctcgccc tcggcggggt ccgtgatgtc
     3781 ccactcaccc gagctcagcg ccactacgtc gtagccgagg cgcgcagcct cggcgctcag
     3841 ccggcgcccg acctgtcctc gggcgccggt gataacgatt cggtccccca tggtcttcga
     3901 gtctggcacg ccgggctcgg ctacggcatt ccgggcgttt ccgccagtag cctggtcgca
     3961 gctaccgaac tgaatggggt cccttcgtgc ctgctgtgcc taccgccggc ccagccgcga
     4021 gcgcgcggcg tggtgtgcgt gtggtctgca cccttgtggc ggtcgcgatc gtgttggcca
     4081 ccggcctggc ttggagcaag atcaaatcgc tggaggacgg catccgtcac atcagctcgc
     4141 cgctgctggg cacgggtggc gaggacggcg cgatcgacat tctgctggtc ggaatggaca
     4201 gccgcaccga cgcgcacggc aacccactga gcgaggagga actcgcccag ctgcatgccg
     4261 gtgacgacgt gtccaccaac accgacacca tcatcctggt gcggatcccc aacaacggga
     4321 agtctgcgac ggccatctcg atcccccgcg actcctatgt tcaggcgccc ggactgggca
     4381 agacgaagat caacggcgtc tacgggcagg tcaaggagga caagcggata cagctcgtcg
     4441 agtcgggggt gagcgacgcg caggccgagt cggaatcaac cgaggcgggc cgggaggtgc
     4501 tgatcaagac cgtcgccgac ctgaccggcg tcaccgtgga ccactacgcg gagatcgggt
     4561 tgctgggctt cgcgttaatc accgacgcca taggcggcgt cgacgtgtgc ttgaaagatg
     4621 aggtgtacga acccctttcc ggcgctgact tccccgcggg ctggcagcgg ctggacggcc
     4681 cgcaggcgct gagcttcgtg cggcaacgcc acgaactgcc gcgtggcgac ctcgaccggg
     4741 tggtgcgtca acaggtcgtg atggcatcgc ttgcccacca agtgatttcg ggcaagacgc
     4801 tgtccagccc ctccgcactg aactccctgc aaaacgcaat ccagcgttcg gtggtgctgt
     4861 ccgacggctg ggacatcatg gatttcatca aggaacttga aaagctgtcc gcgggcaacg
     4921 ttgcgttcgc caccatcccg gtgcttgacg agtcgggctg gagcgacgac ggcatgcaga
     4981 gcgtggtgcg ggtggaccag caccaggtcc aggagtgggt cgggggcctg ctggaagacc
     5041 aggcccaggg caagaccgag gaactggcct acacgccggc caagaccacc gccaacgtgg
     5101 tcaacgacac cgacatcaac gggctggccg cgtcggtgtc agaggtgttg a
//
LOCUS       DASWBF010000005         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_723955,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000005 DASWBF010000000
VERSION     DASWBF010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723955"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..1342
                     /locus_tag="VF874_00090"
     CDS             455..1342
                     /locus_tag="VF874_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9174546.1"
                     /translation="MSGSETHRGILVGVDRSASSMAAVDWAARDAAMRNVALTLMHVV
                     APVVPAVAPWPEIPIPQDYFERQDDKARRILEDARRAVADSTADHGPPFVHSVVLHGP
                     TVSTLVNQSKNADMVVVGCRGEGAFSRVLFGSVSTGLVNYAHCPVAVVHDEAPPPAQA
                     PVLVGIDGSPASELATEIAFDEAFRRGVDLVALHAWSDVSMPDVPGLDLATMEVQAQE
                     ALAEWLAGWQERYPDVTVQRVVVCDQPARQLVEHSESAQLVVVGSNGRGGFAGMLLGS
                     VSTAVAHAARTVVIVARES"
     gene            complement(1422..1550)
                     /locus_tag="VF874_00095"
     CDS             complement(1422..1550)
                     /locus_tag="VF874_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174547.1"
                     /translation="MTIKVAEVGRVSKVDELMARRQRWIDQHHRTREHGLDYGIDL"
     gene            complement(1675..3450)
                     /locus_tag="VF874_00100"
     CDS             complement(1675..3450)
                     /locus_tag="VF874_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX9174548.1"
                     /translation="MATYKGAMHVARNKSRYVTTSGQERVYESVLLRRTYRDGDKVKH
                     ETLANLSNLPGEAVAALEATLKGQTLAPAGGEFSIARSLPHGHVAAVAAMARELGLPA
                     LVGPVGRKRDIVVALIISRVVRPRSKLSTLAWWPDTTLGTDLGVAGASTDEIYAAMDW
                     LAGRQDTIEKKLAAKHLGPDVNPGRMALFDLTSSWVTGRCCALAARGYSRDAKKGCEQ
                     IEYGVLTDPAGRPVAVRVFAGNTADPVAFTEIVTVIRDKLGIRRLVLVGDRGMITSAR
                     IDALRELNDAGTDFGWITALRAPAIAKLAADDGPLQMSLFDTQDLAEITHPDYPGERL
                     IACRNPALAAERARKRHDMLAATEKDLARIAARVTAGTLHGAGKIGETVGKTIGKYNM
                     GKHFRRDITDTTFTYRRDTARIDAEARLDGIYVLRTSVDADTLDPAGIVESYKNLANV
                     ERDFRIIKTDDLDLRPIHHRLDDRVTAHVLICLLACYLIWHLRKAWAPLTFTDEHPPH
                     RDNPVTPARRSPHADTKAARKQDSNANPLRSFRGLLDHLATLTRDRIRYHHSNIEIDK
                     LTDPTPDQRRAFDLLNTPIPLTIAA"
BASE COUNT          564 a         1117 c         1281 g          703 t
ORIGIN      
        1 cgcatccgcg tcgtggacta ccttgagcat gggcgtatcc tcccgaacca gcgcgccaac
       61 gccggctctt gatcagaaca acaggacttt cagatcatcc tcgggaaggt gcgcccactt
      121 acgccgcctc gccgaggctc atccacaggt tctgatcatt gctcgtcatc aagcttgacg
      181 acggtgtggg tgtgagaacg gccccgctcg gcgtggtgag cggggccgtt gtgacggcac
      241 agtcaacttg agcaattcga gggcgatttt cgccggttcg cctccgcgat tcaccaggtc
      301 gcgtgtgacg tatgaggcct tatgtggtta gcagaacgtc cacacgacct caagttgaca
      361 gtgccaatcg gtgaccattg ccacctgatc ttctgcgaag gtcgtcctac ggtcatgcgt
      421 gaagctgcca attcgacaga aacaggggaa cgaaatgtcc ggctccgaaa ctcatcgtgg
      481 cattttggtc ggcgtcgacc gatcagcgtc gtcgatggcg gcggtcgact gggcggctcg
      541 cgatgctgcg atgcgcaatg ttgcgctaac actcatgcac gtcgtcgcac cggtcgtgcc
      601 ggcggttgcg ccgtggccgg agattccgat tcctcaagac tatttcgagc ggcaggacga
      661 caaagcgagg cgaattctcg aagacgcgcg cagggcggtc gccgacagca ccgccgacca
      721 tggcccgccg ttcgtgcaca gcgtggtgct acatggcccg accgtttcca cgctcgtcaa
      781 tcagtccaag aatgcggaca tggtggtcgt gggatgccgg ggcgaaggcg cgtttagtcg
      841 ggtcctgttc ggctcggtca gcaccggcct tgtgaactat gcgcattgcc cggtcgcggt
      901 cgtccatgat gaggccccgc cgcccgcgca ggcaccggtg ctggtgggaa tcgacggttc
      961 gcccgcatcg gaactggcca cggagatcgc ctttgacgag gccttccgcc ggggtgtgga
     1021 cttggtggca ctgcatgcct ggtcggatgt cagcatgccg gacgtgccag gtctggatct
     1081 cgcgacgatg gaagtgcagg cacaagaggc gctggccgag tggctcgccg gatggcagga
     1141 gcgctacccc gatgtcacgg tgcaacgtgt ggtggtgtgc gatcagccgg ctcgtcaact
     1201 tgtcgagcac tcggagtccg cacaactcgt cgtcgtcggc agtaacggtc gcggcggatt
     1261 cgccggcatg ctgctgggct cggtgagcac ggccgtcgcg catgcggcgc ggacggtggt
     1321 gatcgtcgcc cgcgagtcat agcggcgcat gcgggcatca cgctgacccg agctggggcg
     1381 gatgtgctga cggccgcgca ctcctatcag gggtgcgcac gtcacaggtc gataccatag
     1441 tcgaggccgt gctcgcgggt gcggtggtgc tggtcgatcc aacgttgccg ccgcgccatc
     1501 aactcgtcca ctttgctcac cctgccgact tcggcaacct tgattgtcac caccgaagac
     1561 ttggtggggc cgtgggccag gacgcgatcc actaacccga agttacgact gccggggcag
     1621 tgaattcttg ggttgacctg gggattcgct ggtgtgggtg gggtgctgtt ttggctacgc
     1681 ggcgatggtt agggggatcg gggtgttgag gaggtcgaag gcgcggcgtt ggtcgggggt
     1741 ggggtcggtg agtttgtcga tctcgatgtt ggagtggtgg tagcggattc ggtcgcgggt
     1801 gagggtggcc aggtggtcga gtaggccgcg gaagctgcgt agcgggttgg cgttggagtc
     1861 ctgtttgcgg gcggccttcg tgtccgcgtg gggggaacgc cgtgcgggcg tgacggggtt
     1921 gtcccggtgg ggtgggtgtt cgtcggtgaa cgtcagcggt gcccaggcct tgcgtaggtg
     1981 ccagatcagg tagcaggcga gcaggcagat cagcacgtgc gcggtgacgc ggtcgtcgag
     2041 gcggtggtgg atcgggcgca ggtccaggtc gtcggtcttg atgatgcgga agtcgcgttc
     2101 gacgttggcg aggttcttgt agctttcgac gatcccggcg gggtcgaggg tgtcggcgtc
     2161 gacgctggtg cgcaacacgt agatgccgtc caggcgggct tcggcgtcga tgcgggcggt
     2221 gtcgcgacgg taggtgaagg tggtgtcggt gatgtcgcgc cggaagtgct tacccatgtt
     2281 gtatttcccg atcgtcttgc cgacggtttc accgatcttg ccggcgccgt gcagagtgcc
     2341 tgcggtgacc cgggcggcga tgcgggcgag gtctttctcg gtcgcggcca gcatgtcgtg
     2401 gcgtttgcgg gcgcgttcgg cggccagcgc cgggttgcgg caggcgatca accgttcgcc
     2461 ggggtagtcg gggtgggtga tctcggctag gtcctgggta tcgaacaggc tcatctgcag
     2521 cggcccgtcg tcggcggcga gtttggcgat cgcgggtgcg cgcagcgcgg tgatccaccc
     2581 gaaatcggtt ccggcatcgt tgagttcgcg cagcgcgtca atgcgggcgg aggtgatcat
     2641 gccgcggtcg cccaccagca ccagccgccg gatgcccagc ttgtcgcgga tcacggtgac
     2701 gatctcggtg aacgcgaccg ggtcggcggt gttgcccgca aatacgcgca ccgcgaccgg
     2761 tcggccggcc gggtcggtga gcaccccgta ttcgatctgc tcgcagccct tcttcgcgtc
     2821 gcgggagtag ccgcgcgcgg ccagcgcgca gcatcggccg gtcacccacg agctggtcag
     2881 atcgaacagc gccatccggc ccggattcac atccgggccg agatgtttgg ccgcgagctt
     2941 cttttcgatc gtgtcctgcc ggccggccag ccagtccatc gcggcgtaga tctcgtcggt
     3001 ggacgcgccg gccacgccga gatcggtgcc gagagtggtg tcgggccacc aagccagcgt
     3061 ggacagcttc gacctcggcc gcacgacccg cgagatgatc aacgcgacaa cgatatcccg
     3121 tttcctgccc accggaccca ccaacgcggg cagccccaat tcgcgcgcca tcgccgcgac
     3181 cgcggccaca tgcccgtgcg gcagcgaccg ggcgatgctg aactcgccgc cggccggcgc
     3241 caacgtctgg cccttcagcg tcgcctccag cgcggcgacc gcctccccgg gcaggttcga
     3301 caggttcgcc agcgtctcgt gcttcacctt gtcgccgtcg cggtaggtgc gccgcaacag
     3361 caccgactcg tagacccgct cctgcccgga cgtcgtcaca taacgactct tgttccgggc
     3421 tacgtgcatc gcacccttgt atgtcgccat acccgaattc taccgtgatc ccggcgtaaa
     3481 ccacctcaac gacacaccaa ttcagtggct acactcccag acccggatac ccgccgattc
     3541 cccaggtccg ctacacaatc tccgctgaaa acagtcgcaa cttcggccta gccagcaggg
     3601 ctccggcgtt gttgacttgc gacgccgtga cctgcgatga cggacttggg tgacacggcg
     3661 tttcg
//
LOCUS       DASWBF010000006         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_592318,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000006 DASWBF010000000
VERSION     DASWBF010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592318"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..94
                     /locus_tag="VF874_00105"
     CDS             <1..94
                     /locus_tag="VF874_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9174549.1"
                     /translation="PFGLLWVAVDRQRRSLHDIVFGSRVIYARP"
     gene            complement(215..757)
                     /locus_tag="VF874_00110"
                     /pseudo
     CDS             complement(215..757)
                     /locus_tag="VF874_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007538553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            901..1188
                     /locus_tag="VF874_00115"
     CDS             901..1188
                     /locus_tag="VF874_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174550.1"
                     /translation="MTFTGLIASGLTALVVGFAAPAQAAPASPDNAQQAINSLMANGY
                     RVIINKLSDVPLDQAMVVAVRPGRQITQRVTDSGGDSIDKVLYTTVYVDVQ"
     gene            complement(1230..>2485)
                     /locus_tag="VF874_00120"
     CDS             complement(1230..>2485)
                     /locus_tag="VF874_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arabinosyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9174551.1"
                     /translation="VTALYTLWLHFVSRDHGEGRIARVLTAAPIPAAASFMVVVFVGS
                     MMLGVVRQYPTYSNAAANLRVFVGGCGLAADVLVEPDPNNGFLRPVPGNYGPLGPLGG
                     VKPVGFTPNGVPEHIVAEALHMNLPQPGTDYDWDAPTKLAQPGINGSTVPLPYGLEPA
                     RTPVAGSWVDGPQQQSRLTSAWYELPPPDDGHPLVVITAAGTITGKSVLNGYVESQTV
                     ELEFARRGPDGALAPAGRLVPYDLGPTPSWRNLRFARSDIPGDTVAVRVVAEDLSLTP
                     GDWVAVTPPRVPEVRSVQEYVGSSQPVLMDWAVGLAFPCQRPMYHNLGVTEVPAFRIT
                     PDYTAKKQDTDTWEDGRNGGLLGITDLLLRAHVMATYLSDDWGRDWGSLRKFDTIVDA
                     TPATLDLGTATRSGLWKPGVIRIKA"
BASE COUNT          431 a          842 c          802 g          410 t
ORIGIN      
        1 tccgttcggg ttgctctggg tcgcggtcga ccgtcagcgc cggtcgctgc acgacattgt
       61 gttcggcagc agggtgatct acgcgcgacc ctgatcgcgg tagcgtgagc cggtgaccac
      121 gccgctcgcc cgtaccggcg aggacggtcg gcacctcgac acctgcccgc tgtgacaatg
      181 tgccgtcgct ggtgaagggc cgcgaccggt gcacactgct gttgcggttg gtgaagtcgt
      241 tctccggccc cccggagcca gggtccagat acgacggccc cgggccagcc tcgccgatcc
      301 agccggcgtt cgcctggggc gagatggtgt agccgatgtg ctgcaacgtg tacaggacgt
      361 tctgagcgca gtgcttgacg ccgtcctcgt tgccggtgat cagacagccg ccgacgcgtc
      421 cgtagtatag gtactgcccc catcacagag ctgttgtcgc ccaaccaaat cggcccggcc
      481 aacacgagaa tgtccaacgc aaggaccttc tcgaagatca tcggccagtc atcggtagcc
      541 cacccgtggt cacgcatgtc gggccatacc ccggtggcga cgtcgtggtc gaccgcgcgg
      601 atcacctcga cctcggcgcc gtacttttcc atgatctggc gctgatgtcg atcagaccct
      661 gagtgttgct caactccggt gagcgcttga gcgtgcagtt gatgaagagc gcgcgcaggc
      721 cggtgaaatc caactgtcga tcgttcacgc aaggtatgtg tcgtcgtcca gcccaagcgt
      781 tacaccgatg gcgccgtctt gcgttcgtgt aacgccgcgg gatcgcgaac acaccgacat
      841 gtatcagccg cagcagcggc ccgtgaagtt ccacctgaaa ggcgcaaaac aatgaggaag
      901 atgaccttca ccggactgat cgccagcgga ctcaccgcgc ttgtcgtcgg atttgcggca
      961 cccgcgcagg ccgctccagc cagcccggac aacgcacaac aagcgatcaa ctccctgatg
     1021 gccaacggct atcgggtgat catcaacaag ctcagcgacg tcccgctgga ccaggcgatg
     1081 gtggtggcgg tgcggcccgg gcgccagatc actcagcggg tcaccgacag tggtggcgat
     1141 tcgatcgaca aggtgctcta caccacggtc tacgtcgacg tgcagtagcc gcgtaccgaa
     1201 aacacttgtg acaaacctac tttcggcggc taggccttga tgcggatcac gcccggcttc
     1261 cacagcccgc tgcgggtggc ggtgccgagg tcaagtgtcg cgggcgtggc gtcgacgatc
     1321 gtgtcgaact tgcgcagcga cccccagtcc ctgccccagt cgtccgacag atacgtcgcc
     1381 atcacgtgcg cacgcagcag caggtcggtg atgccgagca ggccgccgtt gcggccgtcc
     1441 tcccaggtgt cggtgtcctg cttcttggcg gtgtagtccg gggtgatgcg gaacgcggga
     1501 acctcggtga cgccgaggtt gtggtacatc ggccgctggc acgggaatgc caggcccacg
     1561 gcccagtcca tcaacaccgg ctgtgacgag ccgacgtact cctgcaccga tcgcacctcc
     1621 ggcacgcgcg gcggcgtgac ggcgacccag tcgccaggcg tcaacgacag atcctccgcg
     1681 acgacccgca ccgcgaccgt gtcgcccggg atgtcggacc gcgcgaaccg caggttgcgc
     1741 cacgacggag tcggcccgag gtcgtagggc accagccggc cggccggcgc aagcgcgccg
     1801 tcggggccgc gtcgcgcgaa ctccaactcc actgtctggc tctcgacgta cccgttcagc
     1861 acgctcttac cggtgatcgt tccggcggca gtgatcacga ccaacgggtg tccgtcatcg
     1921 ggcggcggaa gctcgtacca cgccgacgtg agcctgctct gctgttgagg cccgtcgacc
     1981 cagctgccgg ccaccggtgt ccgggccggt tccagcccat acgggagcgg caccgtcgac
     2041 ccgttgatgc ccggctgggc gagcttcgtc ggtgcgtccc agtcgtagtc ggtgcccggc
     2101 tgcggcaggt tcatgtgaag tgcctcggcc acaatgtgtt ccggcacgcc attcggagtg
     2161 aatccgaccg gcttgacccc gcccagcggg cccagcggcc cgtagttgcc gggcacaggc
     2221 cgcaagaagc cgttgttggg gtcgggttcg acgagcacgt cggcggccag gccgcacccg
     2281 ccgacgaata cgcgcaagtt cgcggccgca ttggaatacg tcggatactg ccgcaccaca
     2341 ccaagcatca tcgagcccac gaacaccacc accatgaagc tggccgctgc cgggatcggg
     2401 gccgcggtca acacccgcgc gatccgacct tccccatgat cacgcgacac gaagtgaagc
     2461 cacagggtat acagggcggt gacgg
//
LOCUS       DASWBF010000007         3624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_730, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000007 DASWBF010000000
VERSION     DASWBF010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..692)
                     /locus_tag="VF874_00125"
     CDS             complement(321..692)
                     /locus_tag="VF874_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9174552.1"
                     /translation="MILVDTSVWIDHLHSAESALVELLGRDEVGCHPLVIEELALGSI
                     KQRGDVLELLGHLGVFPDLTHDEVLNLVEKRRLWGQGLSAVDAHLLGSVSLVPGAKLW
                     TRDKRLVAVSRNVGAPLLVER"
     gene            complement(689..886)
                     /locus_tag="VF874_00130"
     CDS             complement(689..886)
                     /locus_tag="VF874_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9174553.1"
                     /translation="MRTTITLDDALVDRASDLTGIREKSALVREGLETLIRVESARRL
                     AALGGSDPSATAAPRRRGRSE"
     gene            complement(1012..2103)
                     /gene="ychF"
                     /locus_tag="VF874_00135"
     CDS             complement(1012..2103)
                     /gene="ychF"
                     /locus_tag="VF874_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX9174554.1"
                     /translation="MSLNLGIVGLPNVGKSTLFNALTRNNVLAANYPFATIEPNEGVV
                     PLPDPRLEKLAEIFDSARIVEAPVTFVDIAGLVRGASEGAGLGNKFLANIRECDAICQ
                     VVRVFSDDDVAHVAGEVDPKSDIEVVETELILADLQTLERALPRLEKEARTHKDRRPL
                     LEAAQAAEKVLNEGTTLFASGLDASLLRELNLLTTKPFLYVFNADEAVLTDSERVAAL
                     RELVAPADAVFLDAKIEAELLELDDESAAELLASIGQNERGLDALARAGFHTLRLQTF
                     LTAGPKEARAWVIHQGDTAPKAAGVIHSDFEKGFIKAEVVSYDDLITAGSMASARAAG
                     KVRMEGKDYVMADGDVVEFRHGATTKPAR"
     gene            2189..3208
                     /locus_tag="VF874_00140"
     CDS             2189..3208
                     /locus_tag="VF874_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6542 domain-containing protein"
                     /protein_id="HEX9174555.1"
                     /translation="MSAQRDGAAVSAQRRSVHPNVPGVPWWGAVMIAVTATSVGFAFD
                     AGGGKELTHVFAALYAIGCIAAVLAVRQSDIFTTVVQPPMILFASVPGAYFLFHGAQV
                     NDLKDIVINCAYPLIERFPLMLFTSGAVLLIGLARWFVGGRAVDTQPAKATAGSTGIA
                     AKFAGIFSRSDADEDDDQKERAPKRRHAVNRPKSTRTAQPARNGKRAEPTRARRARPP
                     GEDDLEPRRRSRTTPSRQSDEDFPRRRSSAHSARNPYERREPYERRAPHDRRSRYDSY
                     DPIEADEVPARRHSGTNGAAASNATHHPISRVRYRGSPTDTDNWERPARSRTRSDSDS
                     WEYDI"
     gene            complement(3341..>3624)
                     /locus_tag="VF874_00145"
     CDS             complement(3341..>3624)
                     /locus_tag="VF874_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9174556.1"
                     /translation="RNSSNSVRLVEVALGAGAHAAHLVDYADDIDPAWLGGVTTIGVT
                     SGASVPEVLVRGVLERLAELGFDVVQPVTTANETLVFALPREIRPFRRG"
BASE COUNT          653 a         1242 c         1106 g          623 t
ORIGIN      
        1 cctacgcgca accattgctc aacgaggact actgattcgg cgaagcgaga gagcttccgc
       61 cggacggtgc gccgccatca gattccgcaa gacttccaca ctccggggcc cggttccaag
      121 ctttctacca gctagtccac agaaactttg gcttcgaacg gcacctaaat ttcgggtggg
      181 caattctgct gaaacgcccc ccggccgggc cgcatcgacc atgtcggtca ttgttgcggg
      241 acgtcgttgc actggagggc cgccgcggtg gccggcagac gttgtttgaa agcggaactc
      301 gaccacgtcg ccgaacaacc tcaccgctcg actagcaagg gagcacccac gttccgactg
      361 acagcgacga ggcgcttgtc gcgagtccac aatttggctc ctggcaccaa cgagaccgaa
      421 ccaagcagat gcgcgtcgac ggcgctcagc ccctgacccc aaaggcgcct tttctcaaca
      481 agattcagaa cctcatcgtg cgtcaggtcc gggaacacgc cgaggtggcc aagtagctcg
      541 agaacgtcgc cgcgctgctt gatcgacccc agagccagct cctcgataac gagcgggtga
      601 caaccaacct cgtctcgccc aagcaactcg acaagcgcgg attccgccga atgaaggtga
      661 tcgatccata ccgacgtgtc gaccaggatc attcgctgcg accgcgtcgt cgcggcgctg
      721 ccgtcgcaga cggatccgaa ccgccgagcg cagcaagccg tcgcgcgctc tcgacacgga
      781 tcaatgtttc gagaccttcg cgcaccagtg cagacttctc ccgtatgccg gtcagatcag
      841 acgcgcgatc gacgagtgcg tcgtcaagtg tgatcgttgt tctcacgata acctcgcata
      901 tgatttcgca tcaattgatg atgcttctga tgctacctta cttgttggga cgtgcggttg
      961 tcggtcatcg atcgagctgg ccggctggga atgtccggcc acgacggcgc gctacctcgc
     1021 tggtttagtc gttgctccat gtcggaactc gaccacgtca ccgtcggcca tcacgtagtc
     1081 cttgccctcc atccggacct tgccggccgc tctggcggag gccatcgagc cggcggtgat
     1141 gaggtcgtcg tacgacacca cctcggcctt gatgaagccc ttctcgaagt cgctgtggat
     1201 cacgccggcg gccttcggcg cggtgtcacc ctggtgaatc acccacgccc gcgcctcttt
     1261 tgggcccgca gtcagaaacg tctgcagccg cagcgtgtga aaacccgctc gcgccaacgc
     1321 atccaggccg cgctcgttct gaccgatcga cgccagcagc tcggcggccg actcgtcgtc
     1381 gagctccagc agctccgcct cgatcttggc gtccaggaac accgcatcgg cgggcgcgac
     1441 gagttcgcgc agcgccgcca cgcgctcaga atcggtcagc acggcctcgt cggcgttgaa
     1501 gacgtacagg aacggcttcg tcgtcaacaa gttcagctcg cgcaacaacg atgcgtccaa
     1561 acccgacgcg aacaacgttg tcccctcgtt cagcaccttc tcggccgcct gggccgcctc
     1621 gagcagcggc cgccggtcct tgtgggtgcg cgcttctttc tccagccggg gcagcgcgcg
     1681 ctccagcgtc tgcaggtctg ccaggatcag ctcggtctcg acgacctcga tgtcggactt
     1741 cgggtccacc tcgcctgcga cgtgcgcgac atcgtcgtcg gagaacaccc gcacgacctg
     1801 acagatcgca tcgcactcac gaatgttggc aaggaacttg ttgcccaggc cggctccctc
     1861 ggatgcgcct ctgaccagac cggcgatgtc gacgaatgtc accggcgctt cgacgatgcg
     1921 ggcggagtcg aatatctcgg ccagcttctc aaggcgcgga tcgggcagcg gcaccacgcc
     1981 ctcgttgggc tcgattgtgg cgaacgggta gttcgcagcc agcacgttgt tacgcgtcag
     2041 cgcgttgaac agcgtcgact tgccgacgtt cggcagaccc acgatcccca ggttcagact
     2101 cacagggtcc acagtctaag caagtgcgac atgcggaccc gggcgtgctt gacagcggag
     2161 tttgcggtcg aaccggtacg gtctacgtgt gtctgcacag cgggacgggg cggccgtgag
     2221 cgcgcagcgc cggtccgtgc atccgaatgt gccgggtgtg ccgtggtggg gcgccgtaat
     2281 gatcgccgtc acagcgacct ccgtggggtt tgcgttcgac gccggcggcg gcaaggaact
     2341 cacacacgtg ttcgctgcgc tctacgcgat cggctgcatc gctgcggtct tggcggtccg
     2401 gcagtccgac attttcacga ccgtcgtgca accgccgatg attctgttcg ccagcgtgcc
     2461 cggcgcctac ttcctgttcc acggcgccca ggtcaacgac ctcaaggaca tcgtcatcaa
     2521 ctgcgcctat ccgctgatcg agcggttccc gctgatgttg ttcacctcgg gcgccgtgct
     2581 gttgatcggg ttggcccggt ggttcgtcgg cggccgggcg gtggacacgc agcctgccaa
     2641 ggccaccgct ggttcgacgg gaatcgcggc gaagttcgcc ggtattttct cgcggtcgga
     2701 tgccgacgag gatgacgacc agaaggaacg ggccccgaag cgccgccacg cagtcaaccg
     2761 gcctaagtcg actcgcacgg cacaacccgc acggaacggt aagcgcgcgg agccgacgcg
     2821 cgcccgtcgt gcccgtccgc cgggtgagga cgacctggag ccccgccgcc ggagccgcac
     2881 tactccgagc aggcaatccg acgaagactt cccacgccga cggtcgtcgg cccactcggc
     2941 gcgcaacccg tacgagcgtc gcgaaccata cgaacgccgt gcgccccacg atcgtcgcag
     3001 ccgatacgac tcctacgatc cgatcgaggc cgacgaagtg ccggcgcgcc ggcattcggg
     3061 aaccaacggg gccgcggcat ccaacgccac acaccatccg atctcccggg tgcgctaccg
     3121 cggcagcccc accgataccg acaactggga gcgcccggcc cgctcccgca cgcgctcaga
     3181 ttcggacagc tgggaatacg acatctaact cgttatcccg ccggaggtga ccacggtgtc
     3241 ctcccaaata gcgatatcgc atatgctatc gcgttcgaaa gatcatgtat cccatggaaa
     3301 ttggtgcacg ggtgcgctgt gtgactcgcg agcggcttgg ctagccgcgg cggaacgggc
     3361 ggatttctcg cggcagcgcg aacaccagcg tctcgttggc ggtggtcacg ggctgcacca
     3421 cgtcgaagcc cagctcggcc agtcgctcca acactccgcg caccaacacc tcgggcaccg
     3481 acgcgcccga cgtgacgccg atggtcgtga cgccgccgag ccacgccgga tcgatgtcgt
     3541 cggcgtagtc gaccagatgg gcggcgtgcg cgcccgcacc gagcgcaacc tcgaccagcc
     3601 gcaccgagtt cgacgaatta cggg
//
LOCUS       DASWBF010000008         7797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_759, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000008 DASWBF010000000
VERSION     DASWBF010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(695..1054)
                     /locus_tag="VF874_00150"
     CDS             complement(695..1054)
                     /locus_tag="VF874_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174557.1"
                     /translation="MPVAVSQRPATTANTEQARALLCGWQKTPGCTSTTLNRCGCALA
                     PTPTQRPAEPTSPPPSPATVFELRAALDDFELRGHAALIDAARRIPANNAWPELARSS
                     PSVATCWAVTSGFVARS"
     gene            complement(1039..1284)
                     /locus_tag="VF874_00155"
     CDS             complement(1039..1284)
                     /locus_tag="VF874_00155"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX9174558.1"
                     /translation="MLELRPNCECCDVDLPPASTEAMICTYECTFCRRCVDGHLMGIC
                     PNCGGNLCPRPIRPAFRLDRDPPSPQRIHAAAPCPLQ"
     gene            complement(1602..1964)
                     /locus_tag="VF874_00160"
     CDS             complement(1602..1964)
                     /locus_tag="VF874_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174559.1"
                     /translation="MQEAAVIGVKDEKWGERPMAFVVRKSDAKVDADAVRAHLLALVE
                     SNRISKYAVPEIDRIAFVAEIPKTSVGKIDRRCCASATRDDGGAPPRWPLRPWRSPSC
                     TNCEPAATNLRQSPVATR"
     gene            2189..2440
                     /locus_tag="VF874_00165"
     CDS             2189..2440
                     /locus_tag="VF874_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174560.1"
                     /translation="MCLAVARHVAHLGVNDPEVDEGHRPPGARAAPHVVVGVGRRDTG
                     RNLCERDDRTGLGNAGAGEDVDALVGRPCTTRAGAPHRR"
     gene            complement(2441..3181)
                     /locus_tag="VF874_00170"
     CDS             complement(2441..3181)
                     /locus_tag="VF874_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9174561.1"
                     /translation="MEGLGVRHGATVAVMDWDSHRYLESYFAIPMMGATLFTVNVRLA
                     PHQIAYTLNDSEAEIVLVHADFVPMLEAIKDELTSVREVVVLADGQAVPANTLPVAGE
                     YEALVGQASPDFEFGDFNENTEAATFYTSGTTGDPKGVYCSHRQIVLHTMATAMTLCA
                     PRAGQRIHREDVYMPLTPMFHALAWGFPFIAVTLGLNRAAGPLPSRGPAQAERVGQGH
                     GVATASRPFCRCCCRRPRRPSRIWPTGR"
     gene            complement(3294..5492)
                     /locus_tag="VF874_00175"
     CDS             complement(3294..5492)
                     /locus_tag="VF874_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9174562.1"
                     /translation="MPIAITPEHKDLADSVRALVERVAPSEVLHEALETPIPNPPPYW
                     KAAADQGLHGVHLAESVGGQGFGILELAVVIAEFGRGAVPGPFVPSVIASALIAAHDP
                     QAKMLGDLASGATIAAYSLESSLTATRQGDTLVIRGEARSVPSAAKASVLVLPVAGLD
                     PETSGTEWVVIDADALEIEPVKSVDPLRPIAHVRANAVEVPADRVLSNLNVAAARALV
                     TTLLSAEAVGVARWATDTAAAYAKIREQFGRPIGQFQGIKHKCAEMAADTERAIAAVW
                     DAARAIDEGLENPDAAAEFAAAVAATLAPTAAQRCAQDCIQVHGGIGFTWEHDTNVYY
                     RRALGIVAAFGRASDYPQLVVDTATSTGMRKLDIDLDPETEKLREEIRAEVAALKALP
                     REQRTAAIAEAGWVSPHLPTPWGRAASPIEQIIIQQEFSGGRVRRPQLGIAAWLVPSI
                     VAFGTDEQKQRFLPPTFRGELIWCQLFSEPGAGSDLASLTTKATKVDGGWRITGQKIW
                     TTAAQVSQWGALLARTDPSAPKHNGITYFLLDMSSDGIEVRPLRELTGNAMFNTVFID
                     DVFVPDELVLGEVNRGWEVSRNTLTAERVSIGSSEAPFLATLDGLVEFLRDGHFDQVA
                     QNRAGRLIAEGHAAKVLNLRSTLLTLAGFDAMPAAAVSKLLSMRTGQGYAEFGVASFG
                     TDGAIGDPGSLPGKWAEYLLFSRATTIYGGTSEVQLNIIAERLLSLPRDP"
     gene            complement(5638..6606)
                     /locus_tag="VF874_00180"
     CDS             complement(5638..6606)
                     /locus_tag="VF874_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX9174563.1"
                     /translation="MDRQTVAVRVLAHFVPGEKVLKILAPHTDWLDVRYCAEDDDTFY
                     RELPGTEVIWHVLRPLSASDLRQGKRLRLVHKLGAGVNTIDVAAATSLGIAVANMPGA
                     NAPSVAEGAVLLMLATLRRLIEMDRLTRTGRGWPTDPSLGETVRDIGSCTVGLVGYGN
                     IARRVEAVVWAMGATVLHTSTSDDGTAGWRGLRALLAESDIVSLHLPLTDATAGLLDR
                     DALAAMKKDAVLVNTSRGAIVDEPALIDALRNGRLAAAGLDVFAVEPVPADNPLLTLG
                     NVVLTPHVTWYTADTMQRYLTMAVENCARLRDGGELANVVNGVGGR"
     gene            complement(6582..7709)
                     /locus_tag="VF874_00185"
     CDS             complement(6582..7709)
                     /locus_tag="VF874_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9174564.1"
                     /translation="MPPPPPGVIREFVGLPSPTARRGGAGGHPCQGLYHRGVGRKPKV
                     AMIATHYQIDFSEHYLADYMATRGIGFLGWNTRFRGYESNFLLDHALVDIGVGVRWLR
                     QVQGVETVVLLGNSGGGSLMAGYQSQANEPNVTPLPGMRPAAGLTELPPADGYVASAA
                     HPGRPDVLTAWMDGAVVEEHDPVATHSDLDIFDERNGPPFPQKFVDRYRAAQVARNDA
                     ITDWVKKELKRVRAAGFSDRPFTVMRTWADPRMVDPTLEPTKRPPNMCYAGVPAKANR
                     SPHGIAAACTLRNWLGMWSLRHAQTRAEPHLARVDCPALVINAEQDTGVFPSDARRIF
                     DALASSDKTLTSIDTDHYFTTPGARSEQADTIAKWIAKRWR"
BASE COUNT         1201 a         2640 c         2619 g         1337 t
ORIGIN      
        1 gcagtgcggg gaatcgggtc gataccacgt cgtgctccat tccgtcggct tgtcctgcat
       61 cctggcggtc gacgtgacgt gaaacccggg acgaaggtcc ccacgattga cttcccgtca
      121 cagtagccgc cgagacttcc ccgtcagagt agccgccgct ggcgatcatc cgggctcgag
      181 tgtgtaccgc gtcaccgaca ttcggaaccg gctcaaggag aacgacatgc ctcggtccgt
      241 tgccgccaag cagccaaacc accggtcggg acaccgtggc caggatgtcc agcggcgtgg
      301 ccactgttga gcggcactcg gtatggggtc cgcagcgtca ctgtggtcat ctgccacgac
      361 ggattcaccc tgcacgatct cgtctcctag agcgtgttgc gccgtagttc gtacgtgcga
      421 tctcccactc gtagttcgaa gtccttcact actgcgctgt tgaacaccga tccaggagtg
      481 ctcagccgca gcccttcgta cacgcatgct ttcgctaggt cgaaccgatt ctgggccaac
      541 tctgctacca cgtcgtgtcg ccaagcagcg ttcgacgggt gcaacatatg ccagaggacc
      601 gcaaggattg cagttctcac cgtgtctagc ccagcaagaa agcacgtcac gatgtcgtcc
      661 cgcgtgctct cggcggaaat gcctgcctcg cggttcaact cctggccaca aaaccgctgg
      721 tcacagccca acacgtcgcg acgctagggg aacttcgggc tagttccggc cacgcgttgt
      781 tggcggggat ccggcgcgcg gcgtcgatga gtgcggcgtg gccgcgcagt tcgaagtcgt
      841 ccagcgcagc gcgcagttcg aacacggtgg cgggcgaggg cggcggtgat gtcggctcgg
      901 cgggcctctg ggtcggtgtg ggtgcgagcg cgcagccgca gcggtttagc gtcgtagaag
      961 tgcatcccgg cgtcttctgc cagccgcaga gcagggcgcg ggcctgctcg gtgttcgcgg
     1021 tggtcgccgg ccgttggctc actgcaacgg gcacggggcc gcggcgtgga tgcgctgcgg
     1081 tgacggcgga tcgcggtcaa gccggaaagc gggtcggatc ggccgggggc acaagttgcc
     1141 gccgcaattg ggacagatac ccattaagtg gccgtcgacg cagcgacggc agaaggtgca
     1201 ctcgtaagtg caaatcatcg cctccgtcga cgcgggcgga agatcgacgt cgcaacattc
     1261 gcagttgggg cgcagctcga gcacacccca ggtctacctg acgatgaccg ccggcacaca
     1321 ccggccgtcg tgtcagcatt cgtaggattt tgcgccggtc acacggtcgg gcgctgtcgg
     1381 caagtgccgc tgacgtacgc cagccggatg cgaggactga ttctgctgct gcggcgtccg
     1441 ccacgatgct tcgccgcgca gccggacagc gctcatctct tgtcgaggtt ccacatccgg
     1501 caatgttcgg aacgcattgg tgtgtagaca gtttcgcgcg tttggctggc ggagtggacg
     1561 tattccgaac gtgcgctact gcttcagcgg gtgtgcgtgt ctcagcgcgt ggctaccggg
     1621 ctttgtctca gattcgtggc ggcgggctcg cagtttgtgc aactcggtga gcgccagggc
     1681 cggagcggcc agcgcggagg cgccccgccg tcgtcacgcg ttgcgctcgc gcagcacctt
     1741 ctgtcgatct tgccgacgct cgtcttcggg atttcggcga cgaaggcgat gcggtcgatc
     1801 tcgggcaccg cgtacttgct gatgcggttt gattcgacca gagcgagcag gtgggcgcgg
     1861 accgcgtctg cgtcgacctt cgcgtcggac ttacggacga cgaacgccat cggccgttcc
     1921 ccccacttct cgtccttgac gccgatgacc gcggcttcct gcaccccgcc acgccgccga
     1981 tcagcgactc gagcccgatg gacgacaccc actcgccgcc ggtcttgatg acgtccttga
     2041 tgcggtcgac gatctgcacc gagccgtccg caaacatgac cgcgacgtcc tgggtgtgca
     2101 ggtagccgcc ggcccacagc tcctcggtgg cctgcggctt attggagtag ccctgggtgc
     2161 tgaagggcga gcgcagcacg atctcacctt gtgccttgcc gtcgcgcggc acgtcgcgca
     2221 tctcggcgtc aacgacccgg aggtcgacga agggcaccgg ccgcccggtg cgcgagcggc
     2281 gccgcacgtc gtcgtcggcg tcggccggcg tgacaccggg cggaatctgt gcgagcgtga
     2341 cgaccggacc ggtctcggaa atgccggagc cggcgaagac gtcgatgccc ttgtcgggcg
     2401 gccctgcaca acgcgggcgg gagcgccgca ccgccgatga tcaccgtcca gtcggccaga
     2461 tcctgcttgg acgcctcggc ctcctgcagc agcatctgca gaatggtcgg gacgcagtgg
     2521 cgacaccgtg accttgtccg actctttcag cttgagcagg tcctcgggaa ggtagcggcc
     2581 cggcagcacg gttcagtccg agcgtgaccg cgatgaacgg gaagccccat gcgagcgcat
     2641 ggaacatcgg cgtgagcggc atgtagacgt cctcgcgatg gatacgctgc ccagcgcgcg
     2701 gcgcgcacaa cgtcatcgcg gtcgccatgg tgtgcagcac gatctggcga tggctgcagt
     2761 agacgccctt cggatcgccg gtcgtgccac tggtgtaaaa ggtggcggcc tcggtgttct
     2821 cgttgaagtc gccgaattcg aagtcgggtg acgcctgccc gacgagcgcc tcatattctc
     2881 ccgcgaccgg cagcgtgttc gccggtaccg cctggccgtc ggcgagcacg accacctcgc
     2941 gcacactcgt cagctcgtcc ttgatggcct cgagcatcgg cacgaaatcg gcgtgcacga
     3001 gcacgatctc ggcttctgaa tcgttgagag tgtaggcaat ctggtgtggc gcgagccgca
     3061 cgttcaccgt gaacagcgtt gcccccatca tcgggatcgc gaagtagctc tccaggtatc
     3121 ggtggctgtc ccagtccatc accgcgacgg tcgccccgtg ccgcacgccc aggccctcca
     3181 gagccgaggc gagctagtca cgccgagttc tgcatcaggg ttgcgatttt tcgagattca
     3241 cagccctggc gcagatttcg gtgagctagg cggcgctgta gagcaaggca gccttacggg
     3301 tcgcggggca ggcttagcag ccgctcggcg atgatgttca gctgcacctc ggaggtgccg
     3361 ccgtagatcg tcgtcgcgcg gctgaacagc aagtactcgg cccacttgcc cggcagcgaa
     3421 cccggatcgc cgatcgcgcc gtcggtgccg aacgaggcca ccccgaactc ggcgtaaccc
     3481 tgaccggtac gcatcgacag cagcttcgac accgcggccg ccggcatcgc gtcgaagccg
     3541 gcgagcgtca gcaacgtcga gcgcaggttg agcactttgg ccgcatgacc ctccgcgatc
     3601 agccggcccg cccggttctg cgccacctgg tcgaagtggc cgtcgcgcag gaactcgacc
     3661 agcccgtcga gggtggccaa gaacggcgcc tcgctgctgc cgatcgaaac ccgttccgcg
     3721 gtcagcgtgt tgcggctgac ctcccagccg cggttcacct caccgagcac cagctcgtct
     3781 ggtacgaaca cgtcgtcgat gaacaccgtg ttgaacatcg cgttgccggt cagctcgcgc
     3841 agtggccgca cctcgatacc gtcgctggac atgtcgagca gaaagtacgt gatgccgttg
     3901 tgttttggcg ccgacgggtc ggtgcgcgcc aacagcgcgc cccactgcga tacctgcgcg
     3961 gccgtcgtcc aaatcttctg gccggtgatc cgccacccgc cgtcgacctt ggtggccttc
     4021 gtggtcaggc tcgccaggtc cgagcccgcg cccggctcgg aaaacagttg gcaccagatc
     4081 aattcgccgc ggaacgtcgg cggcaggaac cgctgcttct gctcgtcggt gccgaacgcg
     4141 acgatcgacg gcaccagcca ggccgcgatg ccgagctgcg gccgcctgac ccggccgccg
     4201 ctgaactcct gctggatgat gatctgctcg atcgggctcg ccgcgcggcc ccacggcgtc
     4261 ggtagatgcg gcgacaccca gccggcctcg gcgatcgcgg ccgtccgctg ctcacgcgga
     4321 agagctttca gcgcagcgac ttccgcgcgg atctcctcac gcagcttctc ggtctcgggg
     4381 tccaggtcga tgtcgagctt gcgcatcccg gtgctcgtcg cggtgtcgac gacgagctgc
     4441 gggtagtccg aggcgcggcc gaacgcggcg acgatgccga gcgcccggcg gtagtacacg
     4501 ttggtgtcgt gctcccaggt gaagccgatg ccgccgtgca cctggatgca gtcctgcgcg
     4561 cagcgttgcg cggcagtcgg cgccagcgtc gcggccactg cggccgcgaa ttccgctgcg
     4621 gcatcagggt tctcgagccc ttcgtcaatc gcgcgggcag cgtcccacac cgccgcgatc
     4681 gcgcgctccg tgtcggcggc catctccgcg cacttgtgct tgatgccctg gaactgcccg
     4741 atcggccggc cgaactgttc gcgaatcttg gcatacgccg cggcggtgtc ggtggcccag
     4801 cgggcgacgc cgacggcctc cgctgacagc agcgtggtca ccaacgctcg cgcggcggcc
     4861 acgttgaggt tgctcagcac ccgatcggcc ggtacctcga ctgcgttggc ccgcacgtgc
     4921 gcgatcggcc gcagcgggtc gacgctcttg accggttcga tctccagtgc gtcggcgtcg
     4981 atgaccaccc actcggtgcc actggtctcg ggatccagac cggcgaccgg caacaccagc
     5041 accgacgctt ttgcggccga cggcaccgag cgcgcctcac cgcggatcac cagcgtgtcg
     5101 ccctgccgcg tcgcggtgag cgacgactcc agcgagtacg ccgcgatcgt cgctccggac
     5161 gccaggtcgc cgagcatctt ggcctgcgga tcgtgcgccg cgatcaacgc gctggcgatc
     5221 accgacggga cgaacggccc gggcactgcg ccgcggccga actcggcgat cacgactgcc
     5281 agctcgagga tcccgaagcc ctgcccgccc accgactcgg ccaagtgcac gccgtgcagc
     5341 ccctggtcgg cggcggcctt ccagtaaggc ggcggattgg ggatcggtgt ctccagcgcc
     5401 tcgtgcagca cctccgatgg cgccacccgt tcgaccaggg cgcgaaccga gtcggccagg
     5461 tctttgtgct cgggcgtgat cgcgataggc ataggcgtcg ttgcctttct cgtgggcctt
     5521 ccgtcgtccg aataaccggt gggttgggaa cgaggttacc cgcatgggaa gaggcactaa
     5581 gcgccggacc gctggggcgc agcacgtcca ttacgcgtgc gaccagggca ccactattca
     5641 gcggcctcct acgccgttga cgacgttcgc caactcaccg ccgtcgcgca gccgtgcgca
     5701 gttctcgacg gccatcgtca agtaccgctg catggtgtct gcggtgtacc acgtgacgtg
     5761 cggtgtgagc accacgttgc ccaatgtcag cagcgggttg tcggccggca ccggctcgac
     5821 ggcgaacacg tccagcccgg cagcggcgag ccggccgttg cgcaacgcgt ctatcaacgc
     5881 gggttcgtcg acgatcgccc cgcgggaggt gttcaccagc accgcgtcct tcttcatggc
     5941 cgccagagcg tcgcggtcga gcagtccggc ggtcgcgtcc gtcagcggaa ggtgcagcga
     6001 cacgatgtcg ctctcggcca gcagagcccg cagcccacgc cagccagcag tcccgtcatc
     6061 agacgtgctg gtgtgcagaa cggtggcgcc catcgcccag acgacggcct ccacccggcg
     6121 cgcgatgttg ccatagccga cgagcccgac ggtgcagctg ccgatgtcgc gcacggtttc
     6181 ccccagcgac gggtcggtcg gccagcccct cccggtccgc gtcaagcggt ccatctcgat
     6241 cagccggcgc agcgtcgcca gcatcagcag cacggcgccc tcggcgaccg acggggcgtt
     6301 ggcgccgggc atgttggcca ccgcgatgcc cagcgacgtc gcggcggcca cgtcgatcgt
     6361 gttcacaccc gcgccgagct tgtgcaccag ccgcagccgc ttgccctgcc ggaggtcgga
     6421 cgccgacagg ggccgcagca cgtgccagat cacctcggtc ccgggcagct cccggtagaa
     6481 ggtgtcgtcg tcctcggcgc agtatcggac gtcaagccaa tcagtatgtg gtgcaaggat
     6541 tttcagcacc ttctcgccgg gtacgaagtg ggcgagtacc cttaccgcca ccgtttggcg
     6601 atccacttgg cgatggtatc ggcctgctcg ctgcgcgccc ccggcgtggt gaagtagtgg
     6661 tcggtgtcga tcgacgtcaa ggtcttgtcg ctgctggcca gcgcgtcgaa gatgcgacgg
     6721 gcgtcggacg ggaacacgcc ggtgtcctgt tctgcgttga tcaccagtgc cgggcaatcg
     6781 acgcgagcca gatgcggctc ggcgcgggtc tgcgcgtgac gcaggctcca catgcccagc
     6841 cagttgcgca acgtgcaggc cgcggcgatg ccgtgcggtg agcggttggc cttcgccggt
     6901 acgccggcgt aacacatgtt cggcggtcgt ttggtgggct ccagcgtcgg gtccaccatt
     6961 ctcgggtcgg cccacgtgcg catcaccgtg aacgggcggt cggaaaagcc cgcggcgcga
     7021 acgcgtttca gttccttttt gacccagtcg gtgatcgcat cgttgcgcgc cacctgagcg
     7081 gcgcggtacc ggtcgacgaa cttctgcggg aacggcggtc cgttgcgttc gtcgaagatg
     7141 tcgaggtcgg aatgcgtggc gacggggtcg tgctcctcga cgacggctcc gtccatccat
     7201 gccgtgagca cgtcgggccg gccggggtgc gcggcactgg ccacgtatcc gtcggcgggc
     7261 ggcagctcgg tcaatcctgc ggcgggccgc atgcccggca gcggcgtcac gttcggctcg
     7321 ttggcctgcg actggtaccc cgccatcagc gatccgccac cggaattccc cagcaacaca
     7381 acagtttcca cgccctgcac ttggcgtagc cagcgcacgc cgacgccgat gtccaccagc
     7441 gcgtggtcga gcaggaagtt gctctcgtac ccgcggaacc gggtgttcca gccgaggaac
     7501 ccgatgcccc gggtggccat gtagtcggcg aggtagtgct cggagaagtc gatctggtag
     7561 tgcgtggcga tcatcgccac cttgggtttg cggcccacgc cgcggtggta gaggccctgg
     7621 cacggatggc cgccggcgcc gccacgccgc gcggtgggcg acggcaggcc gacgaactca
     7681 cggatcactc ccggcggcgg cggcggcatg tcacgcttcc tccttgcaga agatcgctcg
     7741 gtagaagatg ttggccagcg tggtgatgca tgcgtcgtcg tcgacatcgt ccgggtc
//
LOCUS       DASWBF010000009        10271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_724409,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000009 DASWBF010000000
VERSION     DASWBF010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724409"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..152)
                     /locus_tag="VF874_00190"
                     /pseudo
     CDS             complement(1..152)
                     /locus_tag="VF874_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
     gene            complement(175..1701)
                     /locus_tag="VF874_00195"
     CDS             complement(175..1701)
                     /locus_tag="VF874_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9174565.1"
                     /translation="MIAMLRRTRVWLTPLIFLATFAVVLPTAWAAPDPVTTESTVRQP
                     DWGSCQQWLGDMGDLPTARCATVSVPVDYSKPDGAQAQLAVIKIPARGQRLGVLMINP
                     GGPGQSAVDVVAGMASSFGDNEITQHFDLVGFDPRGVGHSTPEVRCRTDAEFDAFRRE
                     PLVDYSPAGVAHIEDIYRQFAAECLNRVGAEFLANVGTVNVAKDMDVVRQALGEAQIN
                     YLGYSYGTQLGTAYAQAFPQNVRAMVLDGAVDPKLDVVEKNVRQMASFQQAFNDFAQE
                     CATIAGCALGADPSKANDRYHALVDPLVAKPGPTLDPRGLSYQDAITGTANSLYTPRF
                     WEFLANGLNALAQGTAAEELLLLADDYWGRAKSGHYTNLQDAFTAIRCVDYVTPTDSA
                     SWVEADRRIREVAPFMSYGQFTGFAPRDVCAFWPVPATTTPYAATSPGPDKVLVVSTT
                     HDPATPYQAGVDLARQLDASLISFDGTQHTVVFKGVGCVDSAVVNYLVNLTAPGDLSC
                     "
     gene            complement(1761..3242)
                     /locus_tag="VF874_00200"
     CDS             complement(1761..3242)
                     /locus_tag="VF874_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9174566.1"
                     /translation="MTCVLTIASCTRVVNGRGEIAHPALGAPIEWGPCQIQSDGDIPR
                     GAECAELAVPADYGNPGSGVATIALIRFRATGDKIGSLVMNPGGPGGSGVLAATWMLR
                     DVPKSLRERFDFVGFDPRGVGMSRPAVWCNSDAENDKERTDPQVDYSPAGVARIEQDT
                     KEFIQRCVDKTGKDFLANVGTASAVKDMDALRAALGDDKLTYIGFSYGTQLGSEYAES
                     FPQKVRAMVFDGAVDPTVEPMDSMLLQSEAFQKAFDDFADDCAKSPDCPLGTDPAKAV
                     DELHSLVDPLVEIPARTDDPRGLSYSDALTAVTSALYSPGLWDKLTDGLTALRHGDPA
                     DDLLELADSYMGRDPDGHYDNSSDAFNAISCVDNPFPTDPPPWVTFDKQRRGVSPWDS
                     YGEFTGDAPRGACAFWPVPTSGRPHSVSAPGLPKVLVISTTHDPATPYEDGVHLAEQL
                     HAALLTVEGTQHTAAFYGDDCVDDIVTRYLVDLTLPPSDARCG"
     gene            complement(3278..4819)
                     /locus_tag="VF874_00205"
     CDS             complement(3278..4819)
                     /locus_tag="VF874_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9174567.1"
                     /translation="MSPTRRLADLARKATTWTLVAATAVAVASCGRVVDGQAKMAMPK
                     VGAPIVWDRCSFTSPDVTPMLASARCGKFGVPVDYSKPDGDSISIAVIKFPATGDKIG
                     SLIINPGGPGESGVNAAIGLAQNFPEQLQERFDLVGFDPRGVRFSKPALWCNSDADND
                     AMRADPMVDYSPAGVAHIEQTTKDFVQRCVDKMGKEFLANVGTANVVKDLDRLRAALG
                     DPKLTYLGYSYGTRIGSTYAEQFPQNVRAMVLDGAIDPNADPFEADIRQAEAFQKAFN
                     DYAADCAKSPDCPLGTDPAKAVEVYHSLVDPLVEKPAPTTDKRGLSYPDAIVGTILPL
                     YSPTLWPNLTAALDELKNGHGDKMLALADQYMGRDEQGRYTNSTDVRSAVNCVDRPPI
                     TDRNRVVEQDRRIREVAPFMSYGEFTGYAPLQTCAFWPVPPTSQPHTIAVKDLPPVLV
                     ISTTGDPATPYQAGVDLSNQLGGALLTFEGTQHTVALEGKECVDDIVTRYLIDLTLPP
                     PDARC"
     gene            complement(4867..5552)
                     /locus_tag="VF874_00210"
                     /pseudo
     CDS             complement(4867..5552)
                     /locus_tag="VF874_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
     gene            5706..6539
                     /gene="panB"
                     /locus_tag="VF874_00215"
     CDS             5706..6539
                     /gene="panB"
                     /locus_tag="VF874_00215"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX9174568.1"
                     /translation="MSEQTVYGAANSARTKIRTHHLQRMKAEGHKWAMLTAYDYSTAR
                     IFDEAGIPVLLVGDSAANVVYGYDTTVPISIDELIPLVRGVVRGAPHALVVADMPFGS
                     YEAGAQQALASATRFMKEAGAHSVKLEGGERVADQIAVLSAAGIPVMAHIGFTPQSVN
                     TLGGFRVQGRGDAAEQTIHDAIAVAEAGAFSVVMEMVPAELATQITGKLTIPTIGIGA
                     GPNCDAQVLVWQDMAGMTSGKTVRFVKRFADIGTELGRAATQYADEVASGVFPAEEHS
                     F"
     gene            6578..8059
                     /locus_tag="VF874_00220"
     CDS             6578..8059
                     /locus_tag="VF874_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEX9174569.1"
                     /translation="MSKHLEVERKFDVVESTVSPSFDGLAAVARVERSPSQSLDAVYY
                     DTPGRDLAAHRITLRRRTGGPDAGWHLKLPAGPDARTEIRAPLGDGDVVPAELRHVVL
                     AIVRDRPLEPVARITTNRAIHQLYGIGGTPLAEFADDQVTAWADGDDTEQYWREWELE
                     LSENAVAAGVADAPLLDRLTNRLLDAGAQPAGHGSKLARVLDSSAPVAVPAPAVDDPV
                     HRAVAEEVGNLLTWDRAVRVDVYDSVHQMRVTTRKIRSLLQASESAFGLSDDAWILDE
                     LRQLASVLGIARDAEVLAERYEKALDELPEELVRGPVRERLIEGARRRYRSGWRKSVA
                     AMRSQRYFRLLDALEGLVAAEPSPPPEGEESPSDATIESGYKRVRKRAKATATAEAEH
                     RNEALHRIRKAAKKLRYTAAAIGEKNVSSAAKNIQSLLGDHQDSVVSRTHLAQQAEAA
                     HAADEDTFTYGLLYQREAEFAQRCRDELGSALKTLDKAAKKAL"
     gene            complement(8061..8446)
                     /gene="rnpB"
                     /locus_tag="VF874_00225"
     ncRNA           complement(8061..8446)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF874_00225"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            8598..9116
                     /locus_tag="VF874_00230"
     CDS             8598..9116
                     /locus_tag="VF874_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174570.1"
                     /translation="MEVREDVFLPELGDFRWLTVGPPGQDESTRHEVLNLVAKGFAGT
                     VFLTTDNCQATYDKMKVRPATAFASASRSKSLRADHDSPSERWWVVGGAGCSAFFEFG
                     EQPPLASEELLVDGPFGGDTFVALLLSGAPASRQHQPASLPWLCGELVDHHFACRRGQ
                     DRKNRLPPGLVV"
     gene            9376..9708
                     /locus_tag="VF874_00235"
     CDS             9376..9708
                     /locus_tag="VF874_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174571.1"
                     /translation="MTTRVPSPETAHRRSRLGGVVRYYGPDHPKVDEARRDLEVSKLA
                     DHVAKVLACWPPPTDEQLDRIAALLRTGTRGRVMPESRKAPAFTRGPIIRHGCGHRNG
                     HRRHGGRR"
     gene            9723..>10271
                     /locus_tag="VF874_00240"
     CDS             9723..>10271
                     /locus_tag="VF874_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174572.1"
                     /translation="MWPTELDTDDEFIELSPLAQWLFKILWLHADLDSGGGIALQVSV
                     WAKASKHVKLEEVEDALGELIACGWVSVDDDTGELWVRPFIRLDASRKPNIFVAAMRA
                     TQTRRSRRLRREAWLEIDRLYQQQPLKPPADDGNEKASKSYRSAVQTRDEAYEQLKAR
                     VLREPFGNRSGTVREPPSVIEPV"
BASE COUNT         1734 a         3307 c         3500 g         1730 t
ORIGIN      
        1 gagacggtga ggatgccttt gtcgtaggtg gctttgatgt cctcctcgtt ggcacccgcc
       61 ggcagggtga ccgagcggac gaatgagcca taggagaact ccgaccggtc cttggattcc
      121 ttcttctcgg tgcgttcggc cttaatcgtc aaccggcctg ataccggtcc ccagctagca
      181 gctcaggtca cctggcgcgg tcaggttcac cagatagttc accaccgcgc tgtccacgca
      241 accgacgccc ttgaacacca cggtgtgctg agtgccgtcg aacgagatca gcgacgcgtc
      301 cagctgacgg gccaagtcga cgccggcttg gtacggcgtc gcggggtcgt gcgtggtgga
      361 cacgacgagc accttgtcgg gccccggcga ggtggccgcg tacggggttg tggtcgccgg
      421 caccggccag aacgcgcaca cgtcgcgtgg cgcgaaaccg gtgaactgac cgtaactcat
      481 gaacggcgcg acctcccgga tccgccggtc ggcttccacc caggatgccg aatcggtggg
      541 cgtcacgtag tcgacgcacc ggatcgcggt gaacgcgtcc tgcaggttgg tgtagtggcc
      601 cgacttggcg cggccccagt aatcgtcggc cagcagcagc agttcttcag ctgcggtgcc
      661 ctgcgccaac gcgttgagac cgttggccaa gaactcccag aagcgcggcg tgtacaagga
      721 gttggccgtg ccggtgatcg catcctgata cgacaggccg cgcgggtcga gcgtcggccc
      781 cggcttggcc accaacggat ccaccagagc gtgatagcgg tcgttcgcct tcgaaggatc
      841 agcgccgagg gcgcaccctg cgatcgtcgc gcactcctga gcgaaatcgt tgaaggcctg
      901 ttggaagctc gccatctggc gcacgttctt ctcgacgacg tcgagcttgg gatcgaccgc
      961 gccgtcgagc accatcgccc ggacgttttg cgggaacgcc tgcgcgtacg cggtcccgag
     1021 ttgcgtgccg tacgagtagc ccaggtaatt gatttgcgcc tcgccgagcg cctgacgcac
     1081 gacgtccatg tctttggcga cgttgacggt gcccacgttg gcgaggaatt cggcgccgac
     1141 ccggttcagg cattccgcgg cgaactgacg gtagatgtcc tcgatgtgcg cgacgccggc
     1201 ggggctgtag tcgacgagcg gctcccggcg gaaggcgtcg aattcggcgt cggtacggca
     1261 ccgcacctcg ggggtggaat ggccgacccc gcgcggatcg aacccgacca ggtcgaagtg
     1321 ttgcgttatc tcgttgtccc cgaacgaaga agccatgccg gcgaccacgt cgacggccga
     1381 ctggccgggt ccgccgggat tgatcatcag cacaccgagc cgctggccgc gcgccgggat
     1441 tttgatcacg gcaagttggg cctgcgcacc gtcgggcttg ctgtagtcga ccggcaccga
     1501 cacggtggcg cagcgcgcgg tgggcagatc acccatgtcg cccagccact gctggcagct
     1561 gccccaatcc ggttgccgga cagtcgattc cgtggttacg gggtcgggcg cagcccatgc
     1621 cgtcggcagc acgacagcga acgtcgccag gaaaataagc ggcgtcaacc acacacgtgt
     1681 ccgccgcaac atggcgatca tgttcgcatg cccccaccgt tcccgccccg ccagtgccca
     1741 tcgtggtagc gaaacgttat ctagccgcat ctcgcgtcgg acggcggcag cgtcaggtcc
     1801 acaagataac gcgtcacgat gtcgtcgacg caatcgtccc cgtagaacgc cgcagtgtgc
     1861 tgagtgccct cgaccgtcag cagcgcggcg tgcagctgct ccgcgaggtg caccccgtcc
     1921 tcatatggcg tggcgggatc gtgcgtggtg gagatgacaa gaaccttcgg cagacccggc
     1981 gcggaaacac tgtgcgggcg gccgctggtc gggaccggcc agaacgcgca cgctcccctc
     2041 ggcgcgtcac cggtgaactc gccatagctg tcccacggcg acactccccg tcgttgcttg
     2101 tcgaacgtca cccacggtgg cgggtccgtc gggaacgggt tgtcgacgca cgagatcgcg
     2161 ttgaacgcgt cggaggagtt gtcgtagtgg ccgtcgggat cgcggcccat gtagctgtcg
     2221 gcaagctcca gcagatcgtc agccgggtcg ccgtgccgca gagccgtcaa cccgtcggtg
     2281 agcttgtccc acaatcccgg cgagtacagg gctgacgtca ccgccgtcag cgcatcggag
     2341 tagctgagtc cgcggggatc gtcggtgcgc gccggtattt ccaccagcgg gtcgacgagc
     2401 gaatgcaatt cgtcgacggc cttggccggg tcggtgccca gcgggcagtc cggcgatttc
     2461 gcgcagtcgt cggcaaagtc gtcgaacgcc ttctgaaatg cctccgactg gagcagcatg
     2521 gaatccatcg gctcgacggt cgggtcgacc gcgccgtcga acaccatcgc gcgcaccttc
     2581 tgcggaaacg attctgcgta ctcagaaccc agttgagttc cgtacgagaa gccgatgtag
     2641 gtcagcttgt cgtcaccgag cgcagcgcgc agcgcgtcca tgtctttgac ggctgaggcg
     2701 gtgccgacgt tggccagaaa gtccttgccg gtcttgtcga cacagcgctg gatgaattcc
     2761 ttggtgtcct gctcgatgcg tgccacgccg gccgggctgt agtcgacttg cgggtcggtg
     2821 cgctccttgt cattctcggc gtcggagttg caccacacgg ccggccgtga cattcccacg
     2881 ccgcggggat cgaagccgac gaagtcgaaa cgctcccgca acgacttcgg cacgtcacgc
     2941 aacatccacg tcgcggccag cactccggag ccgcccggac cgccaggatt cattaccagc
     3001 gaaccgatct tgtcgccggt ggcccggaac ctgatcaatg cgattgtcgc gacgccactt
     3061 ccggggttgc cgtagtcggc cggcacagcc aactcggcgc actccgcgcc ccgcggaatg
     3121 tcgccgtcgg actgaatctg gcagggtccc cactcaattg gcgcgccaag cgccggatgc
     3181 gcgatttccc cgcgaccgtt gacaaccctc gtgcacgacg caatcgtgag cacgcaggtc
     3241 actgctacga cgaatcgggc ccagcggccg atcgcactca acacctggcg tccggcggcg
     3301 gcagggtcaa gtcgatcaaa tagcgcgtga cgatgtcgtc gacgcactcc ttgccctcca
     3361 acgccaccgt gtgctgcgtt ccttcgaacg tcagcagcgc gccgcccaac tggttggaca
     3421 gatcaacacc cgcctgatac ggcgtggccg ggtcccccgt cgtcgaaatc acaagcaccg
     3481 gaggcagatc cttcacggcg atggtgtgcg gttggctcgt cggcggcacc ggccagaacg
     3541 cgcacgtttg cagcggcgcg tacccggtga attcgccgta gctcatgaac ggcgcgacct
     3601 cacgtatccg gcggtcctgc tcgactaccc ggttgcggtc cgtgatgggc gggcggtcaa
     3661 cgcaattcac cgccgagcgc acgtcggtgg agttggtgta gcggccctgc tcgtctcgtc
     3721 ccatgtattg gtcggccagg gcgagcatct tgtctccgtg gccgttcttg agctcgtcga
     3781 gcgcggccgt cagattgggc cacagcgtcg gcgaatacag cggcaagatg gtgccgacga
     3841 tcgcgtcggg atacgacagg ccgcgcttat cggttgtcgg cgcgggcttt tccaccagcg
     3901 ggtccaccag gctgtggtac acctcgacgg ccttcgccgg gtcggtgccg agagggcagt
     3961 cgggcgattt cgcgcagtcg gcggcgtagt cgttgaaggc cttctggaac gcctccgcct
     4021 gccggatgtc ggcctcgaac gggtctgcgt tcgggtcgat cgcgccgtcg agcaccatcg
     4081 cgcgcacgtt ctgcgggaac tgctcggcgt acgtggaccc gatgcgggtt ccgtaggagt
     4141 agcccaggta ggtcagtttc ggatcgccca gcgcggcgcg cagccggtcg aggtccttga
     4201 cgacgttggc ggtgccgacg ttggccagga actctttgcc catcttgtcg acgcagcgct
     4261 ggacgaagtc cttggtcgtc tgttcgatgt gggctacgcc ggcggggctg tagtcgacca
     4321 tcggatcggc acgcatcgcg tcgttgtcgg cgtccgaatt gcaccacagc gccggcttgg
     4381 agaatctgac gccgcgggga tcgaagccga cgaggtcgaa ccgctcctga agttgctccg
     4441 ggaagttctg cgccagcccg atcgccgcgt tgaccccgga ctcgccgggc ccgccggggt
     4501 tgatgatcag cgagccgatc ttgtcgccgg tagccggaaa cttgatgacg gcgatcgaga
     4561 tggaatcgcc gtcgggtttg gagtagtcga ccggcacgcc gaacttgccg catcgggcgc
     4621 tggccagcat cggagtcacg tcgggcgacg tgaacgaaca gcggtcccac acgatcggcg
     4681 cgccgacctt gggcatggcc atcttggcct ggccgtcgac cacccggccg cagctggcga
     4741 ccgcgaccgc cgtcgcggca accagcgtcc aggtcgtcgc cttgcgggcg aggtcggcaa
     4801 ggcgacgagt tgggctcatg gcaccacatg ctgccatgac ggcccgccga tccgccgcag
     4861 caccgatcag ggtttcgcgg cgagcagctc gtcgaggccg cttctgaact tgtcggcgag
     4921 atcccacagg tcgggcagca actcggggca cgacagcagg ccgacgttga gcttgccgtt
     4981 cagcgacatc accgtgatgt tcagaccgga gccgtgaaag atcggcccga gcggatacat
     5041 cgccttgacc tcggcgccca agaaatacag cggcatctgg acatcaggtt ggcaccgctg
     5101 acaccgtcga cgccgacgtg gtgcaccttg gtgatcaccg cgagccggcc gccctgactc
     5161 gggtggtgcc gtcgattccc tcgattaccc acgtctccca cagcggccgg ctgcggtcga
     5221 gtgacagcga ggcgatgtgg ccacagaact cggccagctc cttcgggccg cccggggtag
     5281 gcaggccgat ccggtgcacg tgccggtcga cgtcgaagtc atcgtcgggt gacccacacc
     5341 gggtggtcga ggttgaacgg gctgtcgacg accttctcgc ggaacgtcgg aatgcccctg
     5401 atccgcttgg acagttcgtc tttgagctca gcgaacttgt acccgccggg gatcgtcgag
     5461 gtgtccaact ccaaaatcga gcaaacatgc agcggctgtt gagcggtttc gaggtacaag
     5521 aagctggcgt cgagtccact gagccgtcgc atgccgccga tcgtaggtgg gggccgatat
     5581 gtgaggaagt ccactccacc ctgcgttcac cttctgggtg gtcaagtggc agacttgact
     5641 ggtcagcgaa cccggggacc ctgctttcgg ccacgaggat cgatccgacg aattgaagga
     5701 caacgatgtc tgagcagacg gtttacggtg ctgcgaacag tgcgcgcacc aagatccgca
     5761 cacatcacct gcagagaatg aaggccgagg gccacaagtg ggcgatgctc acggcctacg
     5821 actattcgac cgcgcgcatc tttgacgagg ccggcattcc ggtgctgctg gtcggcgact
     5881 cggccgccaa cgtcgtctac ggctacgaca ccacggtgcc gatctcgatc gacgagctga
     5941 ttccgctggt gcgcggggtg gtgcgcggcg caccgcacgc gctggtggtc gccgacatgc
     6001 cgttcggcag ctacgaggca ggcgcgcagc aggcgctggc cagcgccacc cgcttcatga
     6061 aggaggcggg cgcgcactcg gtcaagctcg agggcggcga gcgggtcgcc gatcagatcg
     6121 ccgtactgag cgcggccggc attccggtga tggcgcacat cggcttcacc ccgcaaagcg
     6181 tgaacacgct cggcggcttc cgggtgcaag gccgcggcga cgcggcggaa cagaccattc
     6241 acgacgcgat cgcggtcgcc gaggccggcg cgttctccgt ggtgatggag atggtgcccg
     6301 cggagttggc cacccagatc accggcaagc tgaccattcc gacgatcggc atcggcgcag
     6361 gacccaattg cgacgcgcag gtgctggtct ggcaggacat ggccgggatg accagcggca
     6421 agacggtgcg tttcgtcaag cgattcgctg acatcggcac cgaattgggg cgtgcggcaa
     6481 cgcaatacgc ggacgaggtg gccagcgggg tgttccccgc cgaggagcac agcttctgac
     6541 ggtaggccgt taaccttggt gtggcacgct gtgcgtgatg tccaagcacc tcgaagttga
     6601 gcggaagttc gacgtcgtcg agtcgacggt gtcgccgtcg ttcgacggtc tcgcggcggt
     6661 ggcgcgggtg gagcgatcac cctctcaatc gctggacgct gtctactacg acacgccggg
     6721 tcgcgatctc gcggctcacc ggatcacact gcgccgacgc accggcgggc ccgacgccgg
     6781 ctggcacctg aagctgcccg cggggccgga tgcccgaacc gagatccgcg caccgttagg
     6841 cgacggcgac gtcgtgccgg ccgagctgcg ccacgtggtg ctggcgatcg tgcgcgaccg
     6901 cccgctggag ccggtggcgc ggatcacgac gaaccgcgcc atccaccagc tgtacggcat
     6961 cggcggcacg ccgctggcgg aattcgccga tgaccaggtg acggcgtggg ctgacggtga
     7021 cgacaccgag cagtactggc gggagtggga gctcgagctg tccgaaaacg ccgtggcggc
     7081 cggggttgcg gacgcaccgc tgctggaccg gttgacgaac cggctgctcg acgcgggtgc
     7141 acagccggcc gggcacggct ccaagttggc gcgggtgctg gatagttcgg cacctgtggc
     7201 tgttccggcg ccggcggtcg acgatcccgt gcaccgggcg gtggccgagg aggtcggaaa
     7261 cctgctgacg tgggatcgtg cggtccgcgt cgatgtgtac gactcggtgc accagatgcg
     7321 ggtgaccacg cgcaagattc gcagtctgct gcaggcctcg gagtcggcat tcgggctgtc
     7381 cgatgacgcg tggatattgg acgaactgcg ccagctggcg agcgtactgg gaattgcccg
     7441 tgacgccgaa gtgctcgccg agcgttacga gaaagcactc gacgaactgc ccgaggagct
     7501 ggtgcgcggg ccggtgcggg agcggctgat cgagggggcg cgccgacggt accggtcagg
     7561 ctggcgaaag tcggtggcgg ccatgcgttc ccagcggtat ttccggctgc tcgacgcgct
     7621 cgaggggctc gtggcggcgg aaccgtctcc gcccccggaa ggcgaggagt cgccgtcgga
     7681 cgccacgatc gagtccggct acaagcgggt gcgcaagcgc gcgaaggcca ccgcgacggc
     7741 cgaagcagag caccggaatg aggcgctgca ccgaattcgc aaggccgcca agaagttgcg
     7801 ctataccgcg gcagcgatcg gcgagaagaa cgtctccagc gcagcgaaaa acatccagtc
     7861 gttgttgggc gatcatcagg acagcgtggt cagccgtaca catctggccc agcaggcgga
     7921 ggccgcgcac gccgccgatg aagacacgtt cacatacggg ctgctgtacc agcgagaggc
     7981 cgagttcgcg caacggtgcc gcgacgaact cggttcggcg ctcaagacac tggacaaggc
     8041 ggcgaagaag gcgctgtagg cggacgagtt ggcctgtaag ccggattctg ttccgcgccg
     8101 tagcgcggcg gcgaccatcc atctgggcac accgtcaccg ggtgcctcaa gcggcctacc
     8161 cgcgtgctcg ggcgagcaac cctcggacgc acgcgcagcc gcggctttcg tcgcggcctc
     8221 ttggccttgc ttcgggtggg gtttaccgag ccattccggt cacccggaat gctggtgcgc
     8281 tcttaccgca ccgtttcacc cttaccaccg cgaacggtgg cggtctgttt tctgtggcac
     8341 tgtcccgcga gtcacctcgg attgccgtta gcaatcaccc tgctctgcga agtccggact
     8401 ttcctcgagt ggtatcgcta ccacccgcgg ccgcccggcc aactcgtccg cacatccata
     8461 ctattccgat gccgcagacc tcgccgacgc gcgctatgcc gaggccatca cggtcgacga
     8521 cccggcggcc gtggcggggc cagcgtctgg cccatgatca ggaagttgca ctcgagtact
     8581 ggaccgagaa ggtcggcatg gaagtccgcg aggacgtttt cctgcccgag ctcggcgact
     8641 tccgttggct gaccgtcggt ccgccgggac aggacgagag cacgcgccac gaggtgctca
     8701 atctcgtcgc gaagggcttc gccggcaccg tgtttctcac caccgacaac tgccaggcga
     8761 cctacgacaa gatgaaagtc cgtccggcaa cagcgttcgc ctcagccagc cgatcgaaat
     8821 cgctgcgggc tgatcatgat tccccatcag aacggtggtg ggtcgttggc ggcgcgggct
     8881 gctcggcgtt tttcgagttc ggcgaacagc caccgttggc gtctgaggag ctgctcgttg
     8941 atggcccgtt cggcggcgat acgttcgtcg cgctcctgct ttcgggtgcg cctgcgtcgc
     9001 ggcagcatca gcccgcgtcc ctcccctggc tctgcggtga gcttgtcgat caccacttcg
     9061 cctgtcggcg tggccaagat cggaagaaca ggctgccacc gggtttggtg gtgtaggtgc
     9121 gcccgctggg gggtgggtgg ttggtaccac agttcgtgaa cgggggtgtg cgacaccgcg
     9181 attggagcgg cggcaacacg ccggtgccga agttcaccgg ctaggtcggc ctgccggggt
     9241 aacctaagcc aaggcgcaca aggtgtgtgt cgtttcgtga gccgggcgat gcgggtgaga
     9301 gaccccgcta gagggcgaat tagcaaccgc cctcggaagt gcggcatagg gagctagccg
     9361 aaaggcggct ccctgatgac gacccgcgta ccctcaccgg aaaccgcgca ccgccgctcg
     9421 cgtctcggcg gtgtcgtccg gtactacggc ccagaccacc ccaaggtgga cgaggcccgc
     9481 cgcgacctcg aagtatccaa gttggccgat cacgtcgcca aggtgctcgc ttgttggccg
     9541 ccgccgaccg atgagcagct ggacaggatc gcggcgctgc tgcgcaccgg cacccggggc
     9601 cgggtgatgc ctgagagccg aaaggccccg gcgttcacac ggggcccaat catccggcac
     9661 ggatgcggtc atcgtaatgg ccaccgccga cacgggggcc ggcgatgagc cgccgtgggg
     9721 ccatgtggcc gaccgagctc gacaccgatg acgagttcat cgagctgtcc ccgctcgccc
     9781 aatggctgtt caagatcttg tggctacacg ccgacctcga cagcggcggc ggtatcgccc
     9841 tccaggtgtc ggtgtgggcg aaggcgtcca aacacgtgaa gctggaggag gtcgaggacg
     9901 cgctgggtga gctgatcgcc tgcggctggg tgtcggtcga cgacgacacc ggggagctgt
     9961 gggtccggcc gttcatccga ctggacgcca gccgcaagcc caacatcttc gtggcggcga
    10021 tgcgcgccac ccagacgcgg cggtcgcgga ggctacggcg tgaggcgtgg ctggagatcg
    10081 accgcctgta tcagcagcag ccgttgaagc caccggccga cgatggcaac gagaaggcgt
    10141 cgaagtcata ccgcagcgcg gtccagacac gtgacgaggc gtacgagcaa ctgaaagcca
    10201 gggtgcttcg agaaccgttc ggtaaccgtt ccggaaccgt tcgggaacct cctagtgtaa
    10261 ttgagcctgt a
//
LOCUS       DASWBF010000010         3086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1185110,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000010 DASWBF010000000
VERSION     DASWBF010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185110"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..2036
                     /gene="istA"
                     /locus_tag="VF874_00245"
     CDS             435..2036
                     /gene="istA"
                     /locus_tag="VF874_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX9174573.1"
                     /translation="MAFREVDVIEVREILRAWLLGKGQRSVARQAGVDRKTVVRYVDA
                     ALEAGLVRDGGQEQLTDELIGQVVAALRPDRPNGHGVAWESLEAQRDQIAKWVGDGLT
                     VVKIGDLLARQGVMVPHRTLHRFCVERTDYQGRGRRATVPVADGEPGEECQVDFARMG
                     LIFDSQAGRRRVVHALIFTAVYSRHMFVWLTFATTLEAIVAGCEAAWRFFGGVFRVLI
                     PDNMKPVVAHADAVNPQFTVGWLEYAQARGFVTDPTRVASPQDKPRVERVVQYVRGNF
                     FAGEQFVDLADAQVRAELWCADKAGLRIHGTTCAQPAVVFAEHEAARLLPAPEVTYRV
                     PIYTEVKVHRDYHVQVDRALYSIPEHLLGQRIQARADDELVKLFYRGQPHPRQQPGGR
                     STDPADLPAEKTAYAMRDLARLISTATGHGPNVGVYAERLLDHELPWTKMRQVYRLLG
                     LVKRYGAEPVDTACGRALDLDVVSVTKIAAMLEKATESGAAPAPRAASGLAPARFARD
                     PAEYRVRPHWLTVIDGGAAGTEAQK"
     gene            2084..2794
                     /locus_tag="VF874_00250"
     CDS             2084..2794
                     /locus_tag="VF874_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9174574.1"
                     /translation="MRQLKLGRMLDTLPERITLAKQQHLSHVAFLELVLADEAARRDT
                     SSAARRARTAGLDPGMRLDTWDDSAAIRYDRALWTELTSLRFLDGPHGACLLGAVGVG
                     KTHLATALGHIAIRRRVPTLMLRADAMFKRLKASRLDNSTEAEMRRLAQVRLLLIDDF
                     ALQPMDATETADFYELVVARHHKASTVTTSNRTPDEWLTMMSDPLLAQSAVDRLTSTA
                     HELVIEGESYRRRQKPSV"
BASE COUNT          486 a          991 c         1077 g          532 t
ORIGIN      
        1 gcccctttca cctattaccg cctcgcgacg gagcccgacc catggtgagc gcccccgcgg
       61 cttttctacg gcacttggcg gtacttgctg gccggcaggg cggtccaggt gcccggtcgc
      121 gtcgagtaca cgggtcagcc ctctgaggct ttaccccgtt gggtgaggga gcctgggcct
      181 gcaacttgac gggcaaacgg cccgtacgct aatcacccgt gatggcggcc gagcgatccg
      241 ggcgcggtca atatgcccct gagcagtggt tagtctgtcg actgccacgt tcatgggacc
      301 ggtttgaccc gagttttgcc acgatcatgg gaccactctt ttgccagtga tgggaccacc
      361 cggcgtgtcg tgccgggtgg ttgtcgcgtt ggcgccttcg gatcgatcta tgtcgatcga
      421 tgtggaggtc cggtatggcg tttcgggagg ttgatgtgat cgaggttcgt gagatcttgc
      481 gggcctggct gttggggaag gggcagcgca gtgtggccag gcaggcgggg gtggaccgca
      541 agacggtggt ccggtacgtg gatgcggcgc tcgaggcagg tttggtccgt gacggtggcc
      601 aggagcagtt gaccgatgag ctgatcgggc aggtggttgc ggcgctgcgc ccggaccgcc
      661 caaacggtca tggcgtggcg tgggaatccc ttgaagcgca acgggatcag atcgccaagt
      721 gggtcggcga cgggttgacg gtggtgaaga tcggtgatct gctcgcccgg cagggggtga
      781 tggtgccgca tcgcacgctg caccggttct gtgtggagcg caccgactac cagggccgtg
      841 gccgtcgcgc gacggtgccg gtggcagacg gtgagcccgg tgaggagtgc caggttgatt
      901 tcgcccggat ggggttgatc ttcgactcgc aggctgggcg gcgccgggtg gtgcacgcgt
      961 tgatcttcac cgcggtgtat tcgcggcata tgttcgtgtg gctgacgttt gcgaccacgt
     1021 tggaggcgat cgtggccggc tgcgaggcgg cgtggcggtt cttcggcggc gtgtttcggg
     1081 tgctcatccc ggataatatg aaacctgttg tggcgcatgc tgatgcggtc aatccgcagt
     1141 tcacggtggg gtggttggag tacgcgcagg cgcgcgggtt tgtcaccgac ccgacccggg
     1201 tggcgtcccc gcaggataag ccgcgcgtgg agcgggtggt gcagtatgtg cgcggcaact
     1261 tcttcgccgg ggagcagttc gtcgatttgg ccgacgcgca ggtccgcgcc gagctctggt
     1321 gtgcggacaa ggccgggctg cgcatccacg gcactacctg cgcgcagccg gcggtggtgt
     1381 tcgccgaaca cgaggcggca cggctgctgc ccgcgcccga ggtgacctac cgggtgccga
     1441 tctacaccga ggtcaaagtc catcgcgact accacgtgca ggtggatcgt gccttgtatt
     1501 cgattccgga acaccttctc ggacaacgca ttcaggctcg cgcggacgac gagttggtga
     1561 agctgtttta ccgcgggcag ccccacccgc gccagcagcc gggcggtcgc tcgacggatc
     1621 cggcggacct gcccgccgag aagaccgcct acgccatgcg ggacctggcc cggctgatca
     1681 gcaccgcgac tggacacggc cccaacgtcg gcgtctacgc cgagcggctg ctcgaccacg
     1741 agctgccctg gacgaagatg cggcaggtct accggctgct ggggctggtg aagcgctacg
     1801 gcgccgagcc ggtggacacc gcgtgcggac gggcgctgga cctcgacgtg gtctccgtga
     1861 cgaagatagc cgcgatgctg gagaaggcca ccgagagcgg cgctgccccc gcaccgcgcg
     1921 cggccagcgg cctggcgccg gcgcggttcg cccgcgaccc cgcagagtac cgggtcaggc
     1981 cgcactggtt gaccgtgatc gacggtggag ccgccggaac ggaggcgcag aagtgacgaa
     2041 aaccgtcgtg cgtgacacga tctcacccga tttgaagcag gtgatgcgcc agctcaagct
     2101 gggccggatg ctcgacactc tgcccgagcg gatcaccctg gccaaacaac agcacctgtc
     2161 gcacgtggcg ttcctcgagc tcgtcctggc cgacgaggcc gcccgccgcg acaccagctc
     2221 tgctgcccgc cgggcccgca ccgccgggct cgatcccggc atgcgcctcg acacctggga
     2281 cgattcggcc gccatccgct acgaccgggc gctgtggacc gagctgacca gcctgcgttt
     2341 cctcgacggc ccgcacgggg cctgcctgct gggcgcggtc ggggtcggca aaacacatct
     2401 ggccaccgcg ctcggccaca tcgccatccg ccgccgggta cccaccctga tgctgcgcgc
     2461 agacgcgatg tttaagcggc tcaaggcatc ccggctcgat aacagcaccg aagccgagat
     2521 gcgccggctc gcccaagtgc ggctgctgct catcgacgac ttcgcgctgc agccgatgga
     2581 cgccaccgag accgccgact tctacgagct tgtcgtggcc cgccaccaca aggcatcgac
     2641 cgtgacgact tcaaaccgaa cacccgacga gtggctgacc atgatgagcg acccgctgct
     2701 cgcccaatcg gcggtcgaca ggctcacctc gaccgcacac gagttggtca tcgaaggcga
     2761 gtcctaccgg cgacgccaga aaccctccgt ttgacaacca gcacgaagga gaacaccatg
     2821 ctccctgagc cgatcaggtg gtcccatgcc actggcaaaa gggtggtccc atcaccgtgg
     2881 caaatgacat cccgtcgcct ttcgtcggcg ttggcttctt gggtcgtcct gcgcccgctg
     2941 gagttgcgcc ttcctcacgg tcggcctacc gacagaatcg atcgccgaac cccaacgggg
     3001 ttgtcatgtt gcgcatgacc aagattcgac cgggcagggc gcccccttga ccccggggac
     3061 ggtggtggct cgcggccggc ggctgt
//
LOCUS       DASWBF010000011         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_790007,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000011 DASWBF010000000
VERSION     DASWBF010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790007"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VF874_00255"
     CDS             complement(<1..908)
                     /locus_tag="VF874_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9174575.1"
                     /translation="MTIVNVELLQSCSSRPDREGRDRLEILTALIDGPSFDAMFRPDV
                     IDVPPEHPIYRWVCIVDGCQRPGSGSGDLCGEHEIAWSRERARGVGKAAFLSAATGLP
                     RFVRVEDTPCRICPDRPIAQSELLMCRSHQMRWFRYQQSVGEAAQFDEWLNNQSPLPG
                     YGTCVVAVCLSRAHAPLGLCARHDARYDRDGRPGGAMLPVRWWNRYERIGEPVPIDYA
                     DKPAFRRWCATTPAPPGGGRINLLGLRPLVAAELKWGLFVHTRRARPQRWQLGWLRSL
                     VTTCRDLELDSLVGLQPDISGCPQMAG"
     gene            complement(905..1300)
                     /locus_tag="VF874_00260"
     CDS             complement(905..1300)
                     /locus_tag="VF874_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9174576.1"
                     /translation="MTGGLIKRVSPAMIHTYFDYVTTEYPCGSNDHGMLLVQLHGGQA
                     GQPWAPVAARRMLARAGQRAGLGLVRPHAFRHSFTSAVLDAAGGNTVIARDAGGWASA
                     TMVDEVYGHVDLHDPVFDAALRTVWGEQR"
     gene            1607..>1759
                     /locus_tag="VF874_00265"
     CDS             1607..>1759
                     /locus_tag="VF874_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9174577.1"
                     /translation="MRAFPVSVPSGQRYWTVLDEDLQVVGVADEYLRHLRFGRDAAES
                     TTKAYAH"
BASE COUNT          265 a          626 c          596 g          272 t
ORIGIN      
        1 ccggccatct gcggacagcc gctgatgtcg ggctgcaagc cgaccagcga gtcgagctcc
       61 aggtcgcggc aggtggtcac cagggaacgg agccacccga gctgccagcg ttgcggccgg
      121 gcgcgccggg tgtggacgaa cagtccccac ttgagctctg cggccaccag cggccgcagc
      181 ccgagcaggt tgatccggcc gccgcccggt ggcgccgggg tggtggcgca ccagcggcgg
      241 aacgctggtt tgtcggcgta gtcgatcggc accggctcgc cgatccgctc gtagcgattc
      301 caccagcgca ccggcagcat cgccccgccc ggacgcccgt cacggtcgta gcgggcgtcg
      361 tggcgcgcgc acaaccccag cggcgcgtgc gcgcggctca gacacaccgc caccacacag
      421 gtcccgtacc cgggcagcgg gctctggttg ttcaaccatt cgtcgaactg ggcggcctcg
      481 ccgaccgact gctggtagcg gaaccagcgc atctggtgcg agcgacacat cagcagctcc
      541 gactgcgcga tcggccggtc cgggcagatc cggcacggcg tgtcctcgac ccgcacgaac
      601 cgcggcaaac ccgttgcggc ggaaaggaac gcggccttgc cgacgccgcg ggcccgctcc
      661 cggctccacg caatctcgtg ctccccgcac agatccccgc tacccgagcc cggccgctgg
      721 cacccgtcga caatgcagac ccaccggtag atcggatgct ccggcgggac atcgatcacg
      781 tccggccgga acatcgcatc gaacgacggc ccgtcgatca acgcggtcag gatttccagc
      841 cggtctcggc cttcccggtc cggtcgagac gaacacgact gcagcagttc cacattcacg
      901 atcgtcaccg ctgctcgccc cacaccgtgc gcaacgcggc gtcgaacacc gggtcgtgca
      961 ggtcgacgtg gccgtagacc tcgtcgacca tcgtcgccga cgcccaccca cccgcgtcac
     1021 gcgcgatcac cgtgttgccg ccggcggcgt cgagcaccgc cgaggtgaac gaatgccgaa
     1081 acgcgtgcgg gcggaccaat ccgagcccgg cgcgctgccc ggcacgggcg agcatccgcc
     1141 gcgccgccac cggcgcccac ggctgtcccg cctggccgcc gtgcagctgc accagcagca
     1201 tcccgtgatc gttgctcccg cacgggtatt cggtggtgac gtaatcgaag taggtgtgga
     1261 tcatcgccgg gctgacccgc ttgatgagcc cacccgtcac ggtgccgtcc tcgacccgcc
     1321 agggatgctt ggtcttcgcc tccgcgtagt tcggattgcc cggccggtgg cagacatgca
     1381 ggtgcggggt gcggcactgc ccgcagttcg catcgtcgcg taggtgcagg tcgaccaggt
     1441 gcagcccgca cagctcgccg atccgcaggc cttctccggg gtttcctgac ctgttgcggg
     1501 acgttgctcg atctgcatga ttgggcgggt tgttcggcgt ttctccgggg gtgtggtgct
     1561 cgtggctggt agtcgtttgt gactgtcgac cagaaagcga ggtgccatgc gagcattccc
     1621 ggtgagtgtg ccgtccgggc aacggtattg gacggtgctg gacgaggacc tgcaggtggt
     1681 tggtgtcgcc gacgagtatc tgcggcatct gcggttcggc cgcgacgcgg ccgagtccac
     1741 gacgaaggcg tatgcgcac
//
LOCUS       DASWBF010000012         6200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_395920,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000012 DASWBF010000000
VERSION     DASWBF010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395920"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1994
                     /locus_tag="VF874_00270"
     CDS             75..1994
                     /locus_tag="VF874_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX9174578.1"
                     /translation="MTVTSAEGGDGGHNVDADLKAAMQQARQALTAGGLPEVAERAWA
                     LMNATPQPPTLVVIGEVKRGKSLLVNALLGDADMSPVDVEIATSAFIRFIPVTGSACE
                     RDTALLFAGGTRKSIDVTELADWVTAPGRHVVDSTVAELPIGAEVAVHGKFLPGVTVV
                     DTPGTGGLNPNHLVLARAASTSANILLMTCDATAPITAQELDFLKSVSAEVDSVLITV
                     TKIDKNYRHWRTIVGENHRLLRERAPRFADVPIVGVSSMRAVAALGIEPGERRDAALR
                     ASGLPELVEHLEQICASVDSLFMANGLHTARSGLDRLMSQLEAQQSAIAGGAQAEAEA
                     ELTAEDQRLRELKKKEGTWRDELARDLNAVQRETLESVDHQLDELRAKWKKRLDETRL
                     DLLRRSPQQAVADITADLEALVAQVSDEYLARVAKLTEECSVEAELAIDAFDTVVRGE
                     DPRKRTHGVVDPTLLSLIVMGSTIVGRQVLPHLGIAAGTSLAFLAGPLTLAVGGAWAA
                     VNLGFRAIRQSRQNMMMWVNTTAMAVNKDLMRAVQERQERIRPVIVNEYKQQLSQAIT
                     EVQKLVSAAQNAAKASQEERDRVVAEIAAEHGALQDIAATLDTELARLSAPAAPEELV
                     PEAESRSDRTPGCPH"
     gene            2016..3147
                     /locus_tag="VF874_00275"
                     /pseudo
     CDS             2016..3147
                     /locus_tag="VF874_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
     gene            complement(3196..4251)
                     /locus_tag="VF874_00280"
     CDS             complement(3196..4251)
                     /locus_tag="VF874_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9174579.1"
                     /translation="MADTLTDDDVAVLRNWIREQGIGSEVGGITPLAGGTQNIVVGLR
                     VDGRRMVLRRPPAHPRPTSNNTMLREIAVLRTLAGSAVPHPEFICGCEDLDVLGVVFY
                     LMDYVDGFNPGTEVAQAYVDKPEFRHDVGLHYAASLAKLGQVPWEGSPLANLKRPGSF
                     LARQVPQFRGFVEAYRQQENYANDSLPGVDRLADWLEDNRPPDSPPGIMHGDAHLNNV
                     LLRRDRPEVAAFVDWEMCTVGEPLLDLSWMLVCWPSGADPLATGAALAAHGGLATRHE
                     LLEAYQSAGGRQTEHLPWYLALACFKLGTVIEGTWARYLAGQATREAGEYLHASAVHL
                     MAMGNEIASGKDLFDSR"
     gene            4397..4726
                     /locus_tag="VF874_00285"
     CDS             4397..4726
                     /locus_tag="VF874_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX9174580.1"
                     /translation="MHEMAITRSVVDAVCEHAAGRRVHSVRVEVGALCAVVPDSMQFC
                     FGLATEGTVAAGARLDLDVQPGRARCRTCGSEFGLSDLFLLCACGSADVEVLTGQDMW
                     ILSMEVS"
     gene            4728..5558
                     /gene="hypB"
                     /locus_tag="VF874_00290"
     CDS             4728..5558
                     /gene="hypB"
                     /locus_tag="VF874_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX9174581.1"
                     /translation="MCATCGCGDDGAVITLAGEHHHHHDHGHGHHDHDHPHEHLPATE
                     TISLEQKVLAKNDALAEENRKWLAERNILAFNVTSSPGAGKTTLLERTIRELQSRRPV
                     SVIEGDQETLLDAERIRATGCRVVQINTGAGCHLDAEMTHRALETLQPEPDSLVFIEN
                     VGNLVCPALFDLGEQTKVVVISVTEGADKPLKYPHMFAAAGLVIINKTDLLPYVDFDL
                     DMCVSYARSINPDVGIMPVSATTGEGMHLWYDWIDVRANSCWPNRSLTSLCARSRPRN
                     "
     gene            5631..>6200
                     /locus_tag="VF874_00295"
     CDS             5631..>6200
                     /locus_tag="VF874_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HEX9174582.1"
                     /translation="MPTEAAVQAEETLIHVLWINAGLSCDGDSVALTAATQPSVEEIA
                     LGALPGLPKVAVHWPLIDFECGPNGGADDFLEWFFKADRGELEPFVLVVEGSIPNEQL
                     HDEGYWCGFGNNPATGQPMTTSEWLDRLAPKATAVVAVGTCATYGGIHAMAGNPTGAM
                     GVPDYLGWDWKSKAGIPIVCIPGCPTHPDN"
BASE COUNT         1126 a         2105 c         2035 g          934 t
ORIGIN      
        1 ccagtgaaca tccgcgcggc aaagacgccg cgacgtaggc tgttggtcgc ggcggagacg
       61 taaggaacgc acccgtgaca gtgaccagcg cggagggcgg ggacggcgga cacaacgtcg
      121 acgccgacct gaaagccgcg atgcagcaag cccgccaggc gctgacagcg ggcggcctgc
      181 ccgaggtcgc cgaacgggca tgggccctga tgaacgcgac gccgcagcca cccacccttg
      241 tcgtcatcgg cgaggtcaag cggggcaaaa gcctgctggt caacgcgctg ctaggcgatg
      301 ccgacatgtc accggtcgat gtagagatcg cgacctcggc cttcatccga ttcattccgg
      361 tcaccggctc ggcctgcgag cgtgacaccg cactactgtt cgccggcggg acccgcaaga
      421 gcatcgacgt caccgaactg gccgactggg tgaccgcccc tggccgccac gtcgtcgact
      481 ccaccgtcgc cgagctgccg atcggcgccg aggtggcagt gcacggcaag tttctgcccg
      541 gagtcaccgt cgtcgacacc cccggaaccg gcggcctgaa ccccaaccat ctggtgctgg
      601 cccgcgcggc cagcaccagc gccaacatct tgctgatgac ctgcgatgcc accgcgccga
      661 tcaccgccca ggagctggac ttcctgaaat cggttagcgc agaggtggat tcggtgctca
      721 tcaccgtcac caagatcgac aagaattatc ggcactggcg cacgatcgtc ggcgagaacc
      781 accggctgct gcgcgagcgc gccccccgat tcgccgacgt gccgattgtc ggcgtctcca
      841 gcatgcgggc ggtcgccgcg ctgggcatcg agcccgggga gcgccgcgac gcggcgttgc
      901 gggcttccgg gctcccggag ctggtcgaac acctcgagca gatctgcgcc tcggtggaca
      961 gcctgttcat ggccaatggg ctgcacaccg cgcggtcggg gctggaccgg ctgatgagcc
     1021 agctcgaggc gcagcagtcg gcgatcgccg gcggagcgca ggcggaggcg gaggcggagc
     1081 tgaccgccga ggaccagcgg ctcagggagc tcaagaagaa agaggggacc tggcgcgacg
     1141 aactcgcgcg cgacttgaac gcggtgcaac gcgagacgct cgaatcggtc gaccaccagc
     1201 tcgatgagct gagagcgaag tggaagaaac ggctcgacga gacgcggttg gacctgctgc
     1261 ggcgctcccc gcagcaggcg gttgccgaca tcaccgccga cctcgaggcc ctggtggccc
     1321 aggtctccga cgaatacctc gcccgggtgg caaaactcac cgaagaatgc tcggtcgagg
     1381 ctgagttggc gatcgacgcc ttcgacaccg tcgtccgcgg cgaagaccct cgaaaacgta
     1441 cccacggcgt ggtcgacccg acgctgctct cgctcatcgt gatgggcagc accatcgtcg
     1501 gccgccaggt gctgccccac ctcggcatcg ccgccggcac ttccctcgcc ttcctcgccg
     1561 gtccgctgac gctggcggtc ggaggcgcct gggccgcggt gaacctgggg ttccgggcca
     1621 tcaggcaaag ccggcagaac atgatgatgt gggtcaacac caccgccatg gcggtgaaca
     1681 aagacctgat gcgcgccgtc caggagcgtc aggagcggat ccggccggtg atcgtcaacg
     1741 agtacaagca gcaactttca caggcgatca ccgaggtgca aaagctggtg agcgccgcgc
     1801 agaatgcagc caaggcgtcg caggaggagc gcgaccgagt cgtcgccgag atcgccgccg
     1861 aacatggcgc gctgcaggac attgccgcga ccctcgacac cgagctggcc aggctgagcg
     1921 caccggcggc gcccgaggag cttgtgcctg aagcggaatc gaggagcgac cgcacgccgg
     1981 gttgtccgca ctgagccgga aggacgttca gcgcgatggc ggcaaaggtt ctgcggttcg
     2041 gtgtgctcgg accgctggaa atgaccgtgg acgcgaagcc ggtcccgctg ggcaccccga
     2101 aacaacgcgc ggtgctggcg atgctcgtca tcaatcgcaa tcagccggtc gcggccagca
     2161 gcctcatcag cgcgacgtgg gaagggtggc ccccgccggg tgccgaggcc tccatgcact
     2221 cctacgtctc cgagattcgc cggctgctcg gcgacgccgg agtcgacccg agccccattc
     2281 tggtcagcac agcccaggga taccagctca gcctccccga aaccgactgc gacctaggca
     2341 gattcgtcgc tgagaagact gccggtgtag tcgccgcggc ggcagggcgt ttcgaacagg
     2401 ccagccggca cctgagcgcg gcgctggccg aatggcgcgg accagtactt gacgacctgc
     2461 ggaattttca cttcgtcgac gcgctggcca ccgcgctcac cgaggacaag gtgatggcgc
     2521 aggccgcccg cgccgaggcc gaaatcgcct gcggcagagg ctacgcggtg atcggcgaac
     2581 tggaggcgct gaccgcccag cacccgtacc gcgaaccggt gagggcgcag ctgatcaccg
     2641 cctactacgt cgccgagcgc cagtccgacg cgctggaagc ctaccgtcgg ttcaaagcca
     2701 ccctcgccca cgacctcggc atcgacccca gccccacggg tgagggtgct gcacgaacgg
     2761 atcctgcgcc aggaaccgct ggacgtccaa caacaggccc gcgccgccgc cgtcggccag
     2821 gtcaccgagc tcgaccggcg caccacgatg ggcgtaggcg gcactgcggc gctgcgcgac
     2881 ccggcgggac atcgccatct gctgcaggcg gtggccaccc ggatcggccg gctggccgac
     2941 aacgaaatcg tcctcgacga cgccaaggtc agccgccacc acgccgtgat catcgacacg
     3001 ggaagccgat acatcatcaa cgacctccgc tccgccaatg gcgtctacgt gcaggacgaa
     3061 cgcgtgcaca ccagcgccac cctcaacgac ggcgaccacg tccgcatctg cacccacgaa
     3121 ttcaccttcg aggcggggcc gaaataacgc gcgccgcagc gccggcccag ccacgactcc
     3181 ctggtgggtg agtagtcacc ggctgtcgaa aaggtctttc cccgaggcga tttcgttgcc
     3241 catcgccatc aggtgcacgg ccgacgcgtg cagatactca ccggcttcgc gggtcgcctg
     3301 gccggccagg tagcgcgccc aggtgccttc gatcaccgtc ccgagcttga agcacgccag
     3361 cgcgaggtac cacggcaggt gctctgtctg gcgacctcct gccgactggt aggcctcgag
     3421 cagttcgtgc cgggtcgcca acccgccgtg ggcggccagt gccgcaccag tggccagtgg
     3481 atcggcaccg ctcggccagc acaccagcat ccagctcagg tccagcagcg gttcgccgac
     3541 cgtgcacatc tcccagtcga cgaacgctgc cacctcgggc cggtcgcggc gcagcagcac
     3601 gttgttcagg tgcgcgtcgc cgtgcatgat tcccggcggc gagtccggcg gacggttgtc
     3661 ctccagccag tccgcgagcc gatcgactcc cggcagggag tcattcgcat agttctcctg
     3721 ctggcggtag gcctcgacga agccgcggaa ctgcggaacc tgccgtgcca gaaaggatcc
     3781 cggccgcttg aggttggcca gcgggctgcc ttcccacggc acctggccca gcttcgccag
     3841 gctcgccgca tagtgcagcc cgacgtcgtg gcggaactcg ggcttgtcga cgtacgcctg
     3901 cgccacctcg gtgcccgggt tgaacccgtc gacgtagtcc atcaggtaga agaccacgcc
     3961 gagaacgtcg agatcctcac agccgcagat gaactcgggg tgcggcaccg cactgccggc
     4021 cagcgtgcgc agcaccgcga tctctcgcag catggtgttg ttgctggtcg gtcgcgggtg
     4081 cgcgggcggc cggcgcaaca ccatccggcg cccgtcgacc cgcaatccca ccacgatgtt
     4141 ctgcgtgccg ccggccagcg gggtgatgcc gccgacctcc gatccgatcc cctgttcgcg
     4201 gatccagttc cgcagcaccg cgacgtcgtc gtcggtcagc gtgtctgcca cgggacgatg
     4261 gtaaggctcg tcgatgagcc tctcgggcga agagcagacc gtcgtcgatg agcctctcgg
     4321 gcgaagagca gaccgtcgtt gtgccccgcg gggcgccgtc ctatggtgag gtcatccggg
     4381 gcgaacgggg aaggctatgc acgagatggc gatcacccga agtgtggtcg atgcggtgtg
     4441 cgagcacgcc gccggtcgtc gcgtgcacag cgtccgggtt gaggtcggcg cgctgtgcgc
     4501 cgtggttccc gactcgatgc agttctgctt cggcctggcc accgaaggca ccgtcgccgc
     4561 cggcgcgcgc ctcgacctcg acgtgcagcc agggcgggcg cgctgccgca cgtgcgggag
     4621 cgagttcggc ttgtcggact tgttcctgct gtgcgcatgc ggcagtgccg acgtggaggt
     4681 cctcaccgga caggacatgt ggattctttc gatggaagtg agttgagatg tgcgctacct
     4741 gcgggtgcgg cgacgacggc gcggtgatca cgctcgccgg agaacaccac caccatcacg
     4801 accacggcca cggccaccac gatcacgacc acccccacga acacctgccg gccaccgaaa
     4861 cgatctcgct ggagcagaag gtactggcca agaacgatgc actggccgag gagaaccgga
     4921 agtggttggc agagcgcaac attctggcgt tcaacgtcac cagctctccc ggcgccggca
     4981 agaccacgtt gctggagcgc accatccgcg agctacagag cagacgaccg gtctcagtca
     5041 tcgagggcga ccaggagacg ctgctggacg ccgagcgcat ccgcgcgacc ggttgccggg
     5101 tggtgcagat caacacgggc gcgggctgtc acctcgacgc cgagatgacg caccgcgcgc
     5161 tggaaactct gcagcccgag cccgactcgc tggtcttcat cgagaacgtc ggaaacctgg
     5221 tctgccccgc cctgttcgac ctcggcgagc aaacaaaagt cgtggtgatc tcggtgaccg
     5281 aaggtgccga caagccgctc aagtatccgc atatgttcgc ggccgccgga ctggtgatca
     5341 tcaataagac ggatctactt ccctacgtgg actttgatct tgatatgtgt gtctcgtacg
     5401 cacgctcaat caatcccgat gtgggcatca tgccggtgtc ggctacgacc ggtgaaggga
     5461 tgcacctgtg gtacgactgg attgacgttc gcgcaaactc atgttggcca aaccggtcgt
     5521 tgacaagtct gtgtgcccgg agtagaccca gaaactagcg ccacgcaagt gggccgacgg
     5581 tcgactcctg tggcgtaggg atcgagtccg tcccggaaag agctgcataa atgccaaccg
     5641 aggctgcagt ccaagcagaa gaaacattga tccatgtgct gtggatcaat gctggtctga
     5701 gttgtgatgg cgattccgtg gcgttgactg ccgccaccca acccagtgtc gaggaaattg
     5761 cgctcggtgc gcttcccggc ctccccaaag tcgccgtcca ttggcccctc atcgatttcg
     5821 aatgcggacc caacggcggt gccgacgact tcctcgaatg gttcttcaaa gctgaccgcg
     5881 gcgaattgga gcccttcgtg cttgtcgtcg agggatccat tcccaacgag cagctgcacg
     5941 acgagggtta ctggtgcggg ttcggcaaca atccagcgac cggtcagccg atgaccacca
     6001 gcgaatggct cgaccggctc gcaccgaaag ccaccgcggt ggtggcggtc ggcacctgcg
     6061 ccacgtacgg cggcatccac gcaatggccg gcaacccgac cggcgccatg ggtgtccccg
     6121 actacctggg gtgggattgg aaatcgaagg ccggcatccc gatcgtctgc atccccggtt
     6181 gccccacgca tccggacaat
//
LOCUS       DASWBF010000013        12677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1118742,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000013 DASWBF010000000
VERSION     DASWBF010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12677
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118742"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1114
                     /locus_tag="VF874_00300"
     CDS             95..1114
                     /locus_tag="VF874_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9174583.1"
                     /translation="MRDVQTTLMFDMRAPHWATPTNELYRAALEMAAFADDIGVDRIN
                     LMEHHGSDDGYLPQPFTLAAGMAAVTNNLRFILGAVLLPLHEPVMVAEQIAITDLISN
                     GRLNVIFGAGYVPFEFAMFGRSLKDRARLMDSGIEIILRALRGEKFEAEGRPVCVRPL
                     PVQKPEDIILVGGGVEASAKRAARHGVGFGPINPALVDVYLEECRRLGREPGQYFRPG
                     PVPISIHLCEDPDQGWAAIEQHAVHVITEYAKWAEQEAQESGRASTSPFKGLTEPAVL
                     RQMGLFAAWTPDELLAKVPEMPDRSTFPFQPLLGGLSPDEGWKSLELLKQTMPKLKAA
                     IAALA"
     gene            1137..1766
                     /locus_tag="VF874_00305"
     CDS             1137..1766
                     /locus_tag="VF874_00305"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX9174584.1"
                     /translation="MLIAIEGVDGAGKRTLGDGLRAAFESNHLSVAGLSFPRYGHSVA
                     ADLAAEALHGQHGDLASSVYAMAVLFAIDRAGAREEIGHLRAAYDVVILDRYVASNAA
                     YSAARLHQDANGEVVDWVRDLEYERLHMPAPDWQVLLDVPAELAADRARHREDNDVTR
                     ARDSYERDDELQRRTVEVYSGLAAEGFGGRWQIAGPDVDPRTLAGQLSR"
     gene            1818..2504
                     /gene="mtrA"
                     /locus_tag="VF874_00310"
     CDS             1818..2504
                     /gene="mtrA"
                     /locus_tag="VF874_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MtrA"
                     /protein_id="HEX9174585.1"
                     /translation="MDTMRQRILVVDDDPSLAEMLTIVLRGEGFDTAVIGDGTQALTA
                     VRELRPDLVLLDLMLPGMNGIDVCRVLRADSGVPIVMLTAKTDTVDVVLGLESGADDY
                     VMKPFKPKELVARVRARLRRNEDEPAEMLSIHDVDIDVPAHKVTRLGEQISLTPLEFD
                     LLVALARKPRQVFTRDVLLEQVWGYRHPADTRLVNVHVQRLRAKVEKDPENPQVVLTV
                     RGVGYKAGPP"
     gene            2527..4188
                     /gene="mtrB"
                     /locus_tag="VF874_00315"
     CDS             2527..4188
                     /gene="mtrB"
                     /locus_tag="VF874_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HEX9174586.1"
                     /translation="MIWGSRRRIRSRWGRAAPFMRGLSALSRAVGLAWRRSLQLRVVT
                     LTLGLSLLVIMALGFVLTSQITDRVLDSKVHAATEEIERARTTVSGIVGGEESRSLDS
                     SLQLARNTLTDRKTDPGAGSNLGGAFDAVLVVPGDGPRAATSAGPVTQIPKALRDFVK
                     AGQVSYQYATVRTEGFTGRALIIGSPTTGPVSNLELYLIYPLSSEESTIALVRGTMLT
                     GGSVLLVLLAAIAVVVSRQVVQPVRSASRIAERFAEGHLSERMPVRGEDDMARLAVSF
                     NDMAESLSRQITQLEEFGNLQRRFTSDVSHELRTPLTTVRMAADLIYDHSEDLDPSLR
                     RSTELMVSELDRFETLLNDLLEISRHDAGVAELSVEQLDLRVTVRSALENVGHLADDA
                     GVEVDVELPEAEVIAEVDPRRVERILRNLIANAIDHAEHKPVQIRMAADDDTVAVTVR
                     DFGVGLRPGEEKLVFSRFWRSDPSRVRRSGGTGLGLAISIEDARLHQGRLEAWGEPGK
                     GACFRLTLPLMRGHKVTTSPLPLKPGAPKNSARPTPRQREPAESV"
     gene            4185..5948
                     /gene="lpqB"
                     /locus_tag="VF874_00320"
     CDS             4185..5948
                     /gene="lpqB"
                     /locus_tag="VF874_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system accessory lipoprotein LpqB"
                     /protein_id="HEX9174587.1"
                     /translation="MRRLVTVVMLVIAVAITGCAGVPSSSAPQAIGTVERSAPPSLPK
                     PTPGMDPDVLLREFLKATADPANRHLAARQFLTESASRGWDDAGSAMLVDNVVFVETR
                     SADRVSVNMRADILGSLSDIGVFETAEGALPDPGSIELLKTSGGWRIDRLPNGVFLDW
                     QQFQETYKRHNLYFADPIGKTVVPDPRYVAVSDPDQLPTELMSKLIAGPRPEMANSVR
                     NMLGAPLRLQGPVTRADGGKTGIGRGYGGARIELEGLSTTDPHSRQLVAAQIIWTFAR
                     ASITGPYVINADGAALDDRFANGWEATDVASTDPGADDGAAAGLHALVGGSLLAVEND
                     NTPRVPGSFGQMPEQRSAMLSRTGRDVASVVALRGGQPDTNASLWIGPSGGVAVEATD
                     GRTITRPSWSLDNAVWVVIDGTNVVRVIQEAASGQPARIPVNATAVTTRSPGPVTELQ
                     LSRDGTRAAMVINGQVILATVEQTPGGEYALTYPRRLGFGLGSSVVSLSWRTGDDIVV
                     SRTDAQHPVSYVNLDGVNSDAPGRNVASPVSTVVANPSTVYIADQRGVLQLSSAAAEN
                     EQRWVDVRPLMIPGAVPVLPG"
     gene            6227..6619
                     /locus_tag="VF874_00325"
     CDS             6227..6619
                     /locus_tag="VF874_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174588.1"
                     /translation="MDRKFVRRDDQYPPPGPSPQQVADAKAKACAAYVTVSNAVSGRA
                     NAPIGSEPEAVAANARLAAAAGQPFLLERLDPATPAPLADAIRKYAANIQDVAMFSLA
                     GVGSDEPAQGARINEWPALNTQITDLCK"
     gene            complement(6769..7575)
                     /locus_tag="VF874_00330"
     CDS             complement(6769..7575)
                     /locus_tag="VF874_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX9174589.1"
                     /translation="MTATVLAVAFAVVIVVALAFVSLTVLREYERGVVFRMGHVRQLY
                     QPGLRFLIPLIDKMIRVDQRVVTLTIPPQEVITRDNVPARVNAVVMFQVTDPLKAILA
                     VENYAVATSQIAQTTLRSLLGRADLDTLLAHREDLNSDLRTIIEKMTQPWGVEVRVVE
                     IKDVEIPESMQRAMAREAEAERERRAKIINARGELQASEELRQAADTLSKSPASLQLR
                     YLQTLLELGADQNSTVVFPLPVDIITPFLKNVQVLARLADDVAPDTSHGR"
     gene            complement(7572..8192)
                     /locus_tag="VF874_00335"
     CDS             complement(7572..8192)
                     /locus_tag="VF874_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174590.1"
                     /translation="MTASVWLVAIYALLLLAIAWGIDLLARRSSSRATRWRTGSFTYH
                     ANHDAWVCPQDQWLWPTSFDPQHRVMRYRASPTVCNSCPVKSTCTTSDHGREVTREVD
                     SWPHSEAGRFHRGIACAIAGMALVMPLAMLIGSHSAADALVLLGASVLVIIAGVPLAR
                     HFWNTPSNAPAHLPHRSGEEEAAAAAIDRYSTRWGGGFAAREDSQQ"
     gene            complement(8189..10522)
                     /locus_tag="VF874_00340"
     CDS             complement(8189..10522)
                     /locus_tag="VF874_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9174591.1"
                     /translation="MAIDPAQSSSAPETDQPSSERLIGKGWVQGVALVMIFGFFVMGI
                     LAYRTYTASMPLPDKVVTESGQVLFTGQQITRGQELFQARGLQEYGSVLGHGAYLGPD
                     FTAEYLRMAADDVAGQFRAEGVADPRDRVVTEFRTNRYNPGTKTVVFTNRQAKAFENI
                     RQHYATYFGEDSNKYGLLPNAISDAADINDLTAFFAWTAWASAAERPGHSYTYTNNWP
                     SEPRVDNGPTGQVVVWSMLSLVALLGGTGIMFALYGRWSQKVGWQSAEAPMLAYRQPG
                     EVPLTPAQRASIWIFAVVSVLFLAQTLVGAAAQHYRADLSTFFGLDLARLLPYNLART
                     WHVQLALFWTAAAFLAGGIFLTPFIARREPRRQSWLVYGLLGAVALVVFGSLITEALS
                     IYGVLPEGSLFSQQWEYLDLPRIWQILLVVGMFFWIVIVWRGMRSRLKGESKLNMPWL
                     FLFAGLAIPAFYAVGLLASSDAHYTVADFWRFWVVHLWVEDFLELFTTVMVAYIFVLL
                     GVVRQRIALGIIFLDIILYSAGGVIGTMHHLYFSGTPVEHMALGAFFSAAEVIPLTFL
                     TVEAWAFLQLGSRQQAGSAHPFPHRWAVMFLVAVGFWNFVGAGIFGFLINLPIVSYYQ
                     IGTALTANHGHAAMMGVYGMLAVALAMFAFRYAIPADKWPEKMARLSFWSMNIGLAWM
                     VFATLLPLGILQLFHSVNDGYVDARSLGYLTQPGNALLEWLRLPGDVILIVGGVLPFI
                     WIAWQALRHFRSGTTAQELPEHPLYTEIETDKAPAEK"
     gene            10677..11306
                     /locus_tag="VF874_00345"
     CDS             10677..11306
                     /locus_tag="VF874_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX9174592.1"
                     /translation="MLDLVLPLECGGCGASATRWCDACARELALKPADPRLINPRVDP
                     RVPVFALGRYAGARRTAIVAAKEHGRSDLIVPLSDALGEGVDRLVGWGIVDRPLTLVP
                     APTRRAAARRRGGDPVARIATAVANHRDGVDVVNALRMRATTRDSVGLSTTARQRNVH
                     GRVRLRRPIGRTVLLVDDIVTTGATAAESVCILQGAGARVAGVLAIAHA"
     gene            11610..12290
                     /gene="raiA"
                     /locus_tag="VF874_00350"
     CDS             11610..12290
                     /gene="raiA"
                     /locus_tag="VF874_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX9174593.1"
                     /translation="MSSRSVESDRELSPEQSDEQLEPTAAAEVVVKGRNVEIPDHFRV
                     YVSQKLARLERFDRSIYLFDVELDHEKNRRQRKNCQHVEITARGRGPVVRGEACADSF
                     YAAFESAVSKLENRLRRSKDRRKVHYGDKTPVSLAEATAAAPPPDTAPAEAASAPADG
                     ATVDEHEPGRIVRTKEHPSTPMTVDDALYEMELVGHDFFLFHDKDSDKPCVVYRRHAY
                     DYGLIRLA"
     gene            12406..>12677
                     /locus_tag="VF874_00355"
     CDS             12406..>12677
                     /locus_tag="VF874_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174594.1"
                     /translation="MRSNAVLDRDGVMAVVERYRGALSELVDLPLDALGVADLFAVLD
                     ATETGRCQTPVIEHQAINRIAAQATPEQIGRSLKKTLAARLRIRPG"
BASE COUNT         2211 a         4177 c         4288 g         2001 t
ORIGIN      
        1 aaggaccagg ccgagtacat cggcgtcgac gtcgacggcc cgtacaaggc tgagcactac
       61 cgctactgat ttctgctggg gttagatcac gtccgtgagg gacgtgcaaa ccacgctgat
      121 gttcgacatg cgcgcgccgc attgggccac gccgacgaac gagctgtacc gggctgcgct
      181 cgaaatggcg gcgttcgctg acgacatcgg ggtggaccgt atcaacctga tggagcacca
      241 cggttccgac gacggctatc tgccgcagcc cttcacgctc gcggccggca tggcggcggt
      301 gacgaacaat ctgcgcttca ttctcggggc ggtgctcctg ccgctgcacg agccggtgat
      361 ggtcgccgaa cagatcgcga tcaccgatct catctccaac ggacgactga acgtcatttt
      421 cggtgccggt tacgtcccgt tcgaattcgc gatgttcggc aggtcgctga aggaccgcgc
      481 gagactgatg gactcgggta tcgaaatcat cctgcgagcg ttgcgcggcg agaagttcga
      541 agcggaaggt cgtcccgtct gcgtccggcc gctgcccgtg cagaagcccg aagacatcat
      601 cctggtcggc ggcggcgtgg aggcgtccgc gaaacgcgcc gccagacacg gtgtcggttt
      661 cgggccgata aaccccgcgc tggtcgacgt ctatctggaa gaatgccgca ggcttggccg
      721 cgaaccgggc caatactttc ggccgggacc cgtgccgata agcatccacc tctgcgagga
      781 ccccgaccaa ggctgggccg ctatcgaaca gcacgcggtt catgtcatca cggaatacgc
      841 caaatgggcc gagcaggagg cgcaggaaag tggcagggcc tccacctcgc cgttcaaggg
      901 gctgacagaa cccgccgtgc tgcgccagat gggattgttc gcggcctgga cgcccgatga
      961 gctgctggcg aaggttcccg agatgcctga ccggtcgacg ttcccgttcc agccgttgct
     1021 cggcggcctg tcgcccgacg aaggctggaa aagcctcgaa ctactcaagc agaccatgcc
     1081 caagctcaag gcggcgattg cggcgctggc ttgattgaat aactgccgtg gcgggcatgc
     1141 tgatcgcgat cgaaggtgtc gacggcgccg gcaagcgcac gctgggcgac ggcctgcgcg
     1201 cggcgttcga aagcaatcac ctctcggtgg ccggcctgtc gttcccgcgc tacgggcatt
     1261 cggtcgctgc cgacctcgcc gccgaggcgc tgcacggcca gcacggcgac ctcgcgtcgt
     1321 cggtgtacgc gatggcggtg ttgttcgcca tcgaccgggc cggcgcccgt gaggagatcg
     1381 gccacctgag agccgcctac gacgtcgtta tcctcgaccg ctacgtcgcg tcgaacgccg
     1441 cgtacagcgc ggcgcgactg catcaggacg ccaacggtga ggtcgtcgac tgggtacggg
     1501 acctcgaata cgagcggctg cacatgcccg cccccgactg gcaggtgctg ctcgacgtgc
     1561 ccgccgagct ggccgccgac cgcgcccgtc accgcgaaga caacgacgta acccgcgcca
     1621 gggactccta cgaacgcgac gacgaactgc agcgccgcac cgtcgaggtg tactccgggt
     1681 tggccgccga gggcttcggc ggacgctggc agatcgcggg tccagacgtc gatccaagga
     1741 ctctcgcggg ccagctcagc cgttagcacg caaaacgccc gtgtggtagc cgggatttat
     1801 cccgatctgg tgacaccatg gacaccatga ggcaaaggat tcttgtggtc gacgacgatc
     1861 cgtcgctcgc cgaaatgctc accatcgtgt tgcgagggga gggtttcgac acggcagtga
     1921 tcggcgacgg cacgcaagcg ctcaccgcgg tgcgcgaact gcgtcccgat ctggtgctgc
     1981 tggacctgat gctgcccggc atgaacggca tcgacgtgtg ccgcgtgctg cgggcggact
     2041 ccggcgtgcc gatcgtgatg ctgaccgcga agaccgacac cgtggacgtc gtgctcgggt
     2101 tggagtccgg cgccgacgac tacgtgatga agccgttcaa gcccaaggag ctggtcgccc
     2161 gtgtgcgcgc ccggctgcgg cgcaacgagg acgagcctgc cgagatgctg tcgatccacg
     2221 acgtcgacat cgacgtgccg gcgcacaagg tgacccggct gggcgagcag atctcgttga
     2281 ccccactgga gttcgacctg ttggtggcgt tggcgcgcaa accgcggcag gtgtttactc
     2341 gtgatgtgct gctcgaacag gtgtgggggt accggcatcc cgccgacacc cgtttggtca
     2401 acgtgcacgt tcagcgactg cgagccaagg tcgagaagga tcctgagaac ccgcaagtgg
     2461 tgctgactgt tcgaggagtg ggatacaagg ccggacctcc gtgatattca ccgctcggcg
     2521 gcggtcgtga tctggggctc gaggcgacgc attcggagtc gttgggggcg ggctgcgccc
     2581 ttcatgcgcg ggctgagtgc gttaagtcga gccgtcggtc tcgcgtggcg tcgatcgctg
     2641 cagctgcgag tggtgaccct gaccctgggg ttgtctcttc tggtgatcat ggctctcggc
     2701 ttcgtgctga ccagccagat caccgaccgc gtgctcgact ccaaggtgca tgccgcaaca
     2761 gaggagatcg agcgggcccg caccaccgtc agcggcatcg tcggtggcga ggagagccgc
     2821 tccctggaca gcagcctgca gctggcccgc aacacgctca ccgaccgcaa gaccgatccc
     2881 ggcgccggca gcaacctggg tggagcgttc gacgccgtgc tcgtggtgcc cggcgacggc
     2941 ccgcgcgcgg cgacgtccgc cgggccggtg acgcagatcc caaaagcttt gcgcgatttc
     3001 gtcaaggccg gacaggtcag ctaccagtac gccaccgtgc gcaccgaggg cttcaccggt
     3061 cgggcgctga tcatcggcag cccgaccacc gggccggtat ccaacctcga gctgtatctc
     3121 atctacccgc tgtccagcga ggagagcacg atcgcgctgg tgcgcgggac catgctcacc
     3181 ggcggctcgg tgctgctggt gcttctcgcg gcgatcgcgg tcgtggtgtc gcggcaggtg
     3241 gtgcaacccg tgcgctcggc gtcgcgaatc gcggagcggt tcgccgaagg acatctgtcc
     3301 gaacgcatgc cggtgcgcgg cgaggacgac atggcgcgac tcgcggtgtc gttcaacgat
     3361 atggccgaaa gcttgtcgcg gcagatcacc caactcgaag agttcggcaa cctgcagcgg
     3421 cggttcacct ctgacgtcag ccacgagctg cgcaccccac tgacgaccgt gcggatggcg
     3481 gccgacctga tctacgacca cagcgaagac ctcgacccgt cgctgcggcg ctcgaccgag
     3541 ctgatggtca gcgagctgga ccgtttcgag acattgctca acgacctgct cgagatttcg
     3601 cggcacgacg cgggtgtggc ggagctgtcc gtcgagcagc tggacctgcg cgtgacggtg
     3661 cgcagtgcgc tcgagaatgt cgggcacctg gccgacgacg cgggcgtcga ggtggacgtg
     3721 gagctgccgg aggccgaagt gatcgccgag gtcgacccgc gccgcgtcga gcggatcctc
     3781 cgcaacttga tcgccaacgc catcgaccat gccgaacaca agccggtgca gatccggatg
     3841 gccgccgacg acgacaccgt ggccgtcacg gtccgcgact tcggggtcgg gctgcgcccc
     3901 ggcgaggaga agctggtgtt cagccgattc tggcgctccg acccgtcgcg agtgcggcgc
     3961 tccggcggca ccgggctggg gctggcgatc agcatcgagg acgcgcggct gcaccagggg
     4021 cggctcgagg cgtggggcga gcccggcaag ggcgcctgct tccggttgac gctgccgctg
     4081 atgcgtggtc acaaggtgac gacgagcccg ctgcccctca aacccggtgc gcccaagaac
     4141 agcgcgcgcc ccaccccgcg ccagcgggag ccggcggaga gcgtatgaga cgcctcgtca
     4201 ccgtcgtgat gcttgtcatc gcggtagcca ttaccggatg tgccggcgtg ccatcatcgt
     4261 cggcgcctca ggccatcgga accgtcgaac gctcggcgcc gccgagcctg cccaagccca
     4321 ccccgggcat ggatcccgac gtcctgctgc gcgagttcct caaggccacg gccgacccgg
     4381 ccaaccgcca tctggccgcg cgccagttcc tgacggagtc ggcgtcgcgc ggctgggacg
     4441 acgccggcag cgcgatgctg gtcgacaacg tggtgttcgt cgaaacccgc agtgccgacc
     4501 gggtttcggt gaacatgcgc gcggacattc tcgggtcgct ctccgacatc ggggtgttcg
     4561 agaccgccga aggtgcccta cccgatcccg ggtcgatcga actgttgaag accagcggcg
     4621 ggtggcgcat cgaccggctg cccaacggcg tgttcttgga ctggcagcag ttccaggaaa
     4681 cctacaagcg ccacaacctg tacttcgccg atccgatcgg caagaccgtc gtgcccgatc
     4741 cgcgctatgt cgcggtgtcg gaccccgatc agctgccgac cgaactgatg agcaagctga
     4801 tcgcgggtcc gcgacccgag atggccaact cggtgcgcaa catgttgggt gcgccgctga
     4861 ggctgcaggg gccggtaacc cgcgccgacg gcggcaagac aggcatcggc cgcggctacg
     4921 gtggcgcccg catcgagctc gaaggcttgt cgaccaccga cccgcacagt cggcaattgg
     4981 ttgcggcaca aatcatttgg actttcgcgc gggccagcat cacgggtccg tacgtgatca
     5041 acgccgacgg cgccgcgctc gacgaccggt tcgccaacgg gtgggaggcc accgacgtcg
     5101 cgtcgacgga ccccggtgcc gacgacggcg ccgcggcggg tctgcatgca ctggtgggcg
     5161 ggtcgctgct ggcggtcgag aacgacaaca cgccgcgggt gcccgggtcg ttcggccaga
     5221 tgcccgagca gaggtcggcg atgctgtcgc gcaccggccg cgacgtggcg tcggtggtcg
     5281 ccctgcgcgg tggccagccg gacacgaatg cgtcgttgtg gatcggaccc agcggcgggg
     5341 tggcggtcga ggcgaccgac ggccgcacca tcacccggcc gagttggtcg ctggacaacg
     5401 cggtatgggt ggtgatcgac ggcaccaacg tggtgcgcgt gatccaggag gccgcgtccg
     5461 ggcagccggc ccgaattccg gtcaatgcca ccgccgtcac cacccggtcc ccgggtcctg
     5521 tcaccgagtt gcagttgtcg cgggacggga cgcgggcggc gatggtgatc aacgggcagg
     5581 tcattctcgc gaccgtcgaa cagacgccgg gcggcgaata cgcgctgacg tacccgcgcc
     5641 ggctgggctt cgggctgggc tcgtcggtgg tgtcgctgtc gtggcgcacc ggcgacgaca
     5701 tcgtcgtcag ccgcaccgat gcgcagcatc cggtgtccta cgtgaacctc gacggggtga
     5761 actccgacgc ccccggccgc aacgtcgcgt cgccggtgtc caccgtggtg gccaacccgt
     5821 caaccgtgta catcgccgac cagcgcggtg tgctgcagct gtcgagcgcg gcggccgaga
     5881 acgagcagcg gtgggtcgac gtgcggccgc tgatgatccc cggcgcggtc ccggtcttgc
     5941 cgggctagcg tgtgtcgccg acgacggtca ggctatgctc tgttgccgtg gcagaggaaa
     6001 caaaaaacgg gccgggggaa tctgcagctt cagagtcgac cgagaccgaa gtcgaggaaa
     6061 cgcaggcctc ggaggcgacc gataccgagg aacccacgga gcgcgcccgc accaggcatt
     6121 ccgcgcagtc gcgtgcgggt ttggccgctc gcgcgctgac tgctgtcgcg ttggtcgtcg
     6181 cattagccgc gctgggtgtt tcgttgtggg tgttgctcaa gaagccgtgg accgaaagtt
     6241 cgtccgccga gacgaccagt acccgccgcc ggggccgagt ccgcagcagg tcgccgacgc
     6301 gaaagcaaag gcctgcgctg cgtacgtgac ggtgagtaat gcggtgagcg gacgcgccaa
     6361 cgcacccatc ggttctgagc cggaggcggt cgccgcgaac gctcggctgg cggcggccgc
     6421 cgggcagccc tttctgctcg agcgcctcga tcccgccacc ccagcgccgc tcgccgacgc
     6481 gatccgcaag tacgcggcca atattcaaga cgtcgcgatg ttttcgctgg ccggggtcgg
     6541 cagcgatgag ccggctcagg gtgcccgcat caacgagtgg ccggcactga acactcagat
     6601 caccgacttg tgtaagtagc tttcgcgccg agcgggaatt tgtgacacgc aattccgccg
     6661 gaagcgtgag ataagtggcc attcggcgga cgcgtagcta tggccgcgac cgtgctgggt
     6721 ggtgaagctg gcattcgggt gaggcactcg acgatgaacg actcccgctc atcggccatg
     6781 gctggtatcc ggtgcgacgt cgtcagccaa gcgtgccaac acctgtacgt tcttcaggaa
     6841 cggagtgatg atgtcgacag gcagcgggaa caccacagtg gagttctggt ccgcgccgag
     6901 ctccaaaagt gtttgcagat atcgcaattg cagtgatgcg gggctcttcg acagcgtgtc
     6961 ggccgcttgc cgcagctctt cggaggcctg cagttcacct cgggcattga tgattttcgc
     7021 gcgccgctca cgctcggctt cggcctcacg cgccatcgca cgctgcatgg attcggggat
     7081 ctccacgtcc ttgatctcca cgacccggac ctccacgccc caaggctgcg tcatcttctc
     7141 gatgatcgtc cgtaggtcgc tgttgaggtc ctcgcggtgg gcgagcaggg tgtcgaggtc
     7201 tgcgcggccg agcagcgacc gcagtgtggt ctgcgcgatc tgcgacgtcg ccaccgcgta
     7261 attctccacg gccagaatgg ctttcagcgg atcggtgacc tgaaacatta cgacggcgtt
     7321 gactcgggcg gggacgttgt cgcgggtgat cacttcctgg ggcgggatgg tcagcgtcac
     7381 cacccgctgg tcgacccgga tcatcttgtc gatgagcggt atcagaaacc gaagtcccgg
     7441 ttggtagagc tgacggacgt ggcccatccg gaagaccact ccccgttcgt attcgcgcaa
     7501 caccgtaagt gagacgaacg ccagcgcgac gacgataacg acggcgaatg cgacggcgag
     7561 cactgttgcg gtcattgttg agagtcttcc ctggcggcga acccgccacc ccaccgggtg
     7621 gagtagcggt cgatcgcggc cgccgccgcc tcctcctcac cggaccggtg cggcaggtgc
     7681 gccggcgcgt tcgacggggt gttccagaag tgccgcgcca gcggcactcc tgcgatgatc
     7741 accagcaccg acgcaccgag cagcaccagc gcgtccgccg ccgaatggct gccgatcagc
     7801 atcgccagcg gcatcacgag cgccatcccg gcgatcgcgc aggcgattcc gcggtggaac
     7861 cgcccggcct cggagtgcgg ccacgagtcg acctcgcggg tgacctcgcg tccgtggtca
     7921 gacgtggtgc aggtggactt gaccgggcag ctgttgcaca ccgtcggcga tgcgcggtaa
     7981 cgcatgaccc ggtgctgtgg atcaaacgac gtcggccaca gccactggtc ctgcgggcag
     8041 acccacgcat cgtggttggc gtggtaggtg aaactacctg tgcgccaccg ggttgcacgc
     8101 gacgacgacc ggcgcgccag caggtcgatg ccccacgcga tcgcgaggag cagcagcgcg
     8161 tagatcgcga cgagccatac cgacgcggtc atttctcggc tggcgcctta tccgtctcga
     8221 tctcggtgta cagcgggtgc tcgggcagct cttgtgcggt ggtcccagaa cggaagtgtc
     8281 gcaacgcttg ccatgcgatc cagatgaacg gcaggacacc gccgacgatg aggatcacgt
     8341 cgccaggcaa ccgcagccac tccagcagcg cattcccggg ctgggtgagg tagcccagcg
     8401 accgcgcgtc gacgtatccg tcgttgaccg agtggaacag ctgcaggatg cccaacggga
     8461 gcagggtcgc gaagaccatc cacgccaagc cgatgttcat cgaccagaat gacagcctgg
     8521 ccatcttttc gggccacttg tcggccggaa tcgcgtaccg gaacgcgaac atcgccaatg
     8581 cgaccgcgag cattccgtac acgcccatca tcgccgcgtg gccgtggttg gcggtcaacg
     8641 cggtgccgat ctggtagtag gaaacgatcg gcaggttgat caggaaaccg aagatgccgg
     8701 cgcctacgaa gttccaaaaa ccaacggcca caaggaacat caccgcccag cgatggggga
     8761 acgggtgggc gcttccggcc tgctggcgcg agccgagctg aaggaacgcc caggcctcga
     8821 cggtcaggaa cgtcaacggg atgacttctg ccgccgagaa gaacgcgccc agcgccatgt
     8881 gctcgacggg ggttcccgag aagtacaggt ggtgcatcgt gccgatgacg ccgcccgcgg
     8941 agtacaggat gatgtctagg aagatgatgc ccagcgcgat ccgctgacgc accacgccga
     9001 gcagcacgaa gatgtaagcc accatcaccg tcgtgaacag ctcgaggaag tcctcgaccc
     9061 acaggtgcac cacccagaac cgccaaaagt ccgcgacggt gtagtgcgcg tcgctgctgg
     9121 cgagtagtcc caccgcgtag aaagcaggaa tcgccaggcc ggcgaacagg aacagccacg
     9181 gcatgttcaa cttcgactcg cccttgagcc gagagcgcat gcctcgccag acgatcacga
     9241 tccagaagaa catccccacg accagaagga tctgccaaat ccgtggcagg tcaaggtatt
     9301 cccactgctg cgagaacagc gatccctccg gcagtacgcc gtagatcgac agcgcttcgg
     9361 tgatcagcga cccgaagacg acgagcgcca ccgcgccgag caatccgtag acaagccagc
     9421 tctgccgccg cggctcgcgc cgagcgatga acggcgtcaa gaagattccg ccggcgagga
     9481 aggccgccgc cgtccagaac aacgccagct ggacatgcca ggtccgggcg aggttgtacg
     9541 gaagcagccg ggccaggtcc aggccgaaga acgtcgacaa gtcggcccgg tagtgctggg
     9601 ccgcggcgcc gaccagggtc tgcgccagga aaagaacgga cacgaccgcg aagatccaga
     9661 tcgacgcgcg ctgcgcgggc gtgagcggca cctcgccggg ctggcggtac gccagcatcg
     9721 gcgcctcggc gctctgccag ccgaccttct gactccaccg cccgtaaagc gcgaacatga
     9781 tgccggtgcc gccgagcagt gcgaccagcg acagcatcga ccacaccacc acctgaccgg
     9841 tcgggccgtt gtcgacgcgc ggctcggatg gccagttgtt ggtgtaggtg tagctgtggc
     9901 cgggacgctc cgcagccgac gcccacgccg tccacgcgaa gaacgcggtc aggtcgttga
     9961 tgtcggcggc gtcgctgatg gcgttgggta acaggccgta tttgttggag tcctcgccga
    10021 agtaggtcgc gtaatgctgc cggatgttct cgaacgcctt ggcctgccgg ttggtgaaca
    10081 cgacggtctt ggtgccgggg ttgtagcggt tggtccggaa ctctgtgacg acgcggtctc
    10141 gcgggtcggc gacgccctcg gcccggaact ggccggccac gtcgtcggcg gccatgcgca
    10201 ggtactcggc ggtgaagtcc ggtccgaggt aggcgccgtg gccgagcacc gacccgtact
    10261 cctgcagccc tctcgcctgg aacagctctt ggccgcgggt gatctgctgg ccggtgaaca
    10321 gcacctgtcc ggattcggtt acgaccttgt ccggcaacgg cattgacgcc gtgtaggtgc
    10381 ggtaggccag gatgcccatc acgaagaagc cgaagatcat caccaaggcg acgccttgga
    10441 cccacccctt gccgatgagg cgttcggagg atggctgatc ggtttccggt gctgacgacg
    10501 actgtgctgg atcgatggcc atacgacgcc ccttgcctcg tgcctgcccg cgtacctcgt
    10561 atgtatcacg gtgggtggtg aaccaaacgc atattcaggg gcttgattgg tgcggggcga
    10621 accttcgata gcaccggtac gaaatcggtg tcggtcgtga tcgccacact ggcctcgtgc
    10681 tcgacctggt gttgccgctc gaatgcggcg gctgcggcgc ctcggccaca cgatggtgcg
    10741 acgcctgcgc gcgcgagctc gctctcaaac cagccgaccc gcgactgatc aacccccgcg
    10801 tggacccgcg agtgccggtg ttcgcgctgg gccgctacgc cggcgcccgc cgcaccgcga
    10861 tcgtggccgc caaggagcac ggccgatcgg atctgatcgt cccgctatca gacgcactgg
    10921 gcgaaggagt ggaccggctc gtcggctggg gcatcgtcga ccgaccgctc accttggtgc
    10981 ccgcacccac ccgtcgggcc gccgcccgcc gccgcggcgg tgacccggtg gcccgcattg
    11041 ccacggccgt cgccaaccac cgcgacggcg tcgacgtcgt aaacgctctg cgcatgcgcg
    11101 cgacgacgcg cgactcggtc ggactgtcga ccacggcccg ccagcgcaat gtccacggcc
    11161 gggtgcggtt acgccgaccc atcggaagaa ccgtgctgct ggtcgacgac atcgtcacca
    11221 ccggcgccac tgccgccgag tccgtctgca tcctgcaagg cgcaggtgcc cgtgtcgccg
    11281 gggtgctggc gatcgcccac gcctgacggc gctgccaatt tctccgccgc caaaccaacg
    11341 tgaagaactg gcaacaggtc tgccaaatcg gtggcacgtg atggtgaaca ggtactacgg
    11401 tcggggcaag tactggttca cggaatccgt gaaacgcggc cgcctcaaca aacgaggcgc
    11461 cacttgccga cagcggtagg aggtgagtct ttcgacctct ggccccggtg ggtggcgaaa
    11521 cggccatcgt tcaaatcccg ccggcccgtc cacagaaccg ggcacgccgg aaccgcgtgc
    11581 gactccgcga cagaaacgag ttgtcaagca tgtcaagccg atccgtggaa tccgaccggg
    11641 agttgtcccc cgagcaatcc gacgagcagc tcgagccgac ggcggcggcc gaggtggtgg
    11701 tgaagggccg caacgtcgag atccccgacc atttccgtgt ctacgtatcg cagaagctcg
    11761 ctcgcctcga gcggtttgac cgcagcatct acctcttcga cgtcgagctc gaccacgaga
    11821 agaaccgccg ccagcgcaag aactgccagc acgtggagat cacggcacgc ggccgcggcc
    11881 cggtggtccg cggcgaggcc tgcgccgaca gcttctacgc cgccttcgag tcggcggtca
    11941 gcaagctgga gaaccggctg cggcgcagca aagaccggcg caaggttcac tacggcgaca
    12001 agaccccggt gtcactcgcg gaagccaccg ctgcggcccc gccgccggac accgcccccg
    12061 cggaggcagc ctcggccccg gccgacggcg ccactgtcga cgagcatgag cccggccgca
    12121 tcgtgcgcac gaaggagcac ccgtcgaccc cgatgaccgt cgacgacgcg ctctacgaga
    12181 tggagctggt cggccacgac ttcttcctgt tccacgacaa ggacagcgac aagccgtgcg
    12241 tcgtgtaccg ccgccatgcc tacgactacg gtctgatccg gctcgcctga cggcgccacc
    12301 agcacaaccc tctgacaagt cggggacacg gaacaggttt gtccacagcc ttttcggctg
    12361 tggatgaagc cgccgcttgt cggtgggcgg ggatatcatt cgaatatgcg ttcgaacgcg
    12421 gtgctggatc gggacggggt gatggccgtc gtcgagcgct accgcggggc gctgtcggag
    12481 ttggtggatc tgccgttgga cgcgctgggc gtggctgatc tgttcgcggt gctcgacgcc
    12541 accgaaaccg gccgctgcca aaccccggtg atcgagcacc aggccatcaa ccgcatcgcc
    12601 gcgcaggcca cccccgagca gatcggcaga tcactgaaga agacgctggc cgcccggctg
    12661 cgcatccggc ccggcga
//
LOCUS       DASWBF010000014        10573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_922095,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000014 DASWBF010000000
VERSION     DASWBF010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10573
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922095"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..264)
                     /locus_tag="VF874_00360"
     CDS             complement(79..264)
                     /locus_tag="VF874_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9174595.1"
                     /translation="MDAHHCDVLVVGAGPVGLAAAAELRRRGVDVRCVDAAAHHNVST
                     KALGLQARTLELFERLG"
     gene            complement(264..1283)
                     /locus_tag="VF874_00365"
     CDS             complement(264..1283)
                     /locus_tag="VF874_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9174596.1"
                     /translation="MKFGVDVPTCLAGMVYPVPFATADDVVRIGVEAEQLGYHEVAGN
                     DHLSTQRFVREAWPAAPDYFEPLMMLATIAAKTSVVRLGTGILVLPMRDPVLLAKQVA
                     TLDHISGGRVTLAVAVGGYRDEFEAVVPDLADANRTHLMAEGIEALRVLFEKPRSTYE
                     GKYRRFLDVESYPKPVQSPLPIFSGGNGKGALQRAGERCEGWLPAKLGPAEIASGRAK
                     VADYAAAAGRDPSTITIGLQSVVCLADTAEQARERLEKSTFELFRTSLKDTMMKGVDL
                     DKYVADNLIGTPDDVCEKVAAFEKAGLDGFFATLFVANTVDEMLEQMRLFARYVLPAF
                     PDGGR"
     gene            complement(1337..4081)
                     /locus_tag="VF874_00370"
     CDS             complement(1337..4081)
                     /locus_tag="VF874_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9174597.1"
                     /translation="MLHGRAAELADIERLLNGGRSGHSSVLVIQGEAGSGKTALLDHV
                     AAHAKGFEVLRCAGVESEAELPFAALQFLLRDVLDRLDTLPEPQAAALRAAFGLAQAP
                     GVDRFLAGLATLTLLSEVAADGPLLCVIDDTQWLDRASIDTLLFAGRRLGAEGIVLLL
                     AVRDFGVAEDFRGLTVLGLPGLSQSAASALLAERAADLASGVRDRLIGEATGNPLALI
                     ELAAALRRAESVTAGPPAVTEGLSIGGRRVLDAFGAQLDGLAADTRLALLVAAAEDTG
                     ELGVVLAAAGRIGRSLADLEPAERAQLIRLTNESITFRHPLIRSAVYTRSSVGERLAV
                     HRALADAVDEPEYADRHAWHLAAAAAGYDDEAAAAMAAAADRAVLRGGYAASAAAMER
                     AARLTADPAVRGRRLAAAAMNSRDSAQLDRAASLAEEASTLTEDRRTLAQLAWVRARV
                     EFERGTPRHASEMLLDGAVTVYHTDREEAARMLVEVVRMAYFADEAPHLSRAIEIMNG
                     LVLPADHPLRPMLQATSILAKLQSDRPEDQVASLLPAVRAIRPDQLGMTIGNLPIHSA
                     FLRMVIGDADEASAQTERMLTEARARGLIGGLPHILLQHAQAALMAGRLQEALRAATE
                     GIQIAEDTGQHHSAANLRGVLARISAMTGDEERCVVLATEAMRRGSERQSSGVGLAAL
                     AVAVLDLGYGRYAEALQRLTALPSRLQRHPTLAYLAPPEWAEAAARSGHPEQAAEVMT
                     WYELWASHRKNPVVQANLHRCRALLGADAEAEEHYRAAARLYRDTNRPMARARTELLY
                     GEWLRRCRRRSEAREPLRDALRVFEETGAHSWAERARTELRATGETVASAAQQASGAA
                     HLTPQELQVVRLAATGMSNRDIGAQLFISPRTVGYHLYKAFPKLGVGGRYELAALNLA
                     "
     gene            complement(4195..4710)
                     /locus_tag="VF874_00375"
     CDS             complement(4195..4710)
                     /locus_tag="VF874_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9174598.1"
                     /translation="MAERSAKLVVNTAGPSKDVRPPQIDDIDRRILKALHDDARIPNT
                     ALADMVGIAPSTCHGRLRRLQDIGVIRGFYTDVDPAAIGRPLQAMISVSLQSKARGRI
                     RHFISEIRKLPQVIDVYFLAGADDFILHVAARDTDDLRAFVVENLNADPDVAGTQTSL
                     IFEHLRGGAPL"
     gene            4802..5917
                     /gene="ald"
                     /locus_tag="VF874_00380"
     CDS             4802..5917
                     /gene="ald"
                     /locus_tag="VF874_00380"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX9174599.1"
                     /translation="MRVGVPTEIKNNEYRVAITPAGVSELTRRGHDVVIQAGAGEGSA
                     ISDTDFKAAGAEIVSGAEHIWEQADLLLKVKEPIEPEYALMRRGQTLFTYLHLAASQP
                     CTDALMRSGVTSIAYETVQTADGALPLLAPMSEVAGRLAAQVGAYHLMKPAGGRGVLM
                     GGVPGVGPADVVVIGGGTAGYNAARVASGMGAHVTVLDVSLAKLRELDAEFNGRIRTR
                     HSSALELEGAVKRADLVIGAVLVPGAKAPKLVSNSLVSQMKPGAVLVDIAIDQGGCFE
                     DSRPTTHDDPTFPVHGTVFYCVANMPGAVPRTSTFALTNATMPYVIALADRGWQAACR
                     ADSALAEGLSTHEGALLNDQVAHDLGLPFTDPAKVLA"
     gene            complement(5922..6320)
                     /locus_tag="VF874_00385"
     CDS             complement(5922..6320)
                     /locus_tag="VF874_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEX9174600.1"
                     /translation="MIVDTSVWIDYFVAPQSTAGRWLADRIAVGATVIVPEVVLMELL
                     IGTTDESKAAMRRRLPQRFDIEPLAPLRDAEDAAAIHRRCRRRGHTVRSLIDCQVAAM
                     AMRLGEAVAHRDRDFEVISAHCGLRTEPLF"
     gene            complement(6321..6548)
                     /locus_tag="VF874_00390"
     CDS             complement(6321..6548)
                     /locus_tag="VF874_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019283586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9174601.1"
                     /translation="MSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEALTRD
                     EALAMQGSGFDYTNDEIESFSDAEPRRGTES"
     gene            complement(6597..7487)
                     /gene="bla"
                     /locus_tag="VF874_00395"
     CDS             complement(6597..7487)
                     /gene="bla"
                     /locus_tag="VF874_00395"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEX9174602.1"
                     /translation="MGELSRRRIIVVGATLFAAACTAKPTLKDPAEPMPPLDERITAL
                     ERRHNARVGLFAADLGSGRTAEFRADDMFAMCSTFKTYAGASVLQRCDRGELHLSDQV
                     FVNPVAILPNSPVTESNAGHAMTLSDLCAAALQRSDNCAANLLLTTIGGPSEITAFAR
                     SIGDDKTRLDRWELELNTALPGDPRDTTTPRAFARGYQNLLTGSALAVPSRQQLDEWM
                     RANVTSSMRAGLPQGWTSADKTGSGDYASTNDVGIAYGPDGRKVLLAMMTRTASDDPK
                     AENLRPLIGELTKQIVSTLS"
     gene            complement(7480..8286)
                     /locus_tag="VF874_00400"
     CDS             complement(7480..8286)
                     /locus_tag="VF874_00400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9174603.1"
                     /translation="MDIEGRVAIVTGAGSGIGRALATALAEAGARVVVGDIDAAAAQQ
                     TASMISGHAVPRQADASAEADIAALIALAATEFGSVDIYVANAGIGGPPGLGMSDADW
                     DRVLDVNLRAHIRAATQLLPQWVERGSGYFVSVASAAGLLTQLGAAGYAVSKHAALGF
                     AEWLAITYGDDGVGVSCVCPMGVDTQLLTELRESADPAGQLAGRSVISAGEVLEPARV
                     ADVTVEAVRQGRFLVLPHAEVIDMYRQKGADYDAWIAGMRRYRRRLTADG"
     gene            complement(8294..9598)
                     /locus_tag="VF874_00405"
     CDS             complement(8294..9598)
                     /locus_tag="VF874_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9174604.1"
                     /translation="MPRIPAPDVGAGLRRTTLPGGLRVVTECLPGVHSASVGVWVGVG
                     SRDEGASVAGAAHFLEHLLFKATPTRTAVQIAQAVDAVGGELNAFTAKEHTCYYAHVL
                     DSDLELAVELVADVVLNGVCAPDDVELERDVVLEEIAMRDDDPEDAIGDVLLAALFGR
                     HPVGRPVVGTVDSVSSMTRAQLHSFHVRRYTPERMVVAVAGNVDHDQVVSLVRRYFGA
                     RLERGRMPVPPRKGAGRVAGRPALTLCRRDAEQTHMTLGVRCPGRHWEHRWALAVLNT
                     AIGGGLSSRLFQQIREQRGLAYTVYSTVDTFAESGALSVYTACLPERFGDVVGVTTSV
                     LETVARDGITADECRIAKGSLRGGLVLGLEDSASRMNRIGRSELNYGEHRSIGHTLAQ
                     IDSVTLDEVNAVARRVLTGPYGAAVLGPYQAKRQLPQPLRKL"
     gene            complement(9576..>10573)
                     /locus_tag="VF874_00410"
     CDS             complement(9576..>10573)
                     /locus_tag="VF874_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX9174605.1"
                     /translation="TGRVGSPKRREIGHGALAERALVPVLPNRDEFPYAIRQVSEALG
                     SNGSTSMGSVCASTLGLLNAGVPLKAPVAGIAMGLVSDDVELEDGGTERRFVALTDIL
                     GAEDAFGDMDFKGAGTKDFVTALQLDTKLAGIPSQVLANALSQAKDARLTILEVMAEA
                     IDAPDEMSPYAPRVTTMKVPVDKIGEVIGPKGKMINSITEETGAQISIEDDGTVFVGA
                     TDGLSAQAAIDKINAIANPQLPKVGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLG
                     RGKRIAKVEDVVKVGDKLRVEIADIDNRGKISLVLVAEEEAADAPAPADAAHTGA"
BASE COUNT         1644 a         3723 c         3495 g         1711 t
ORIGIN      
        1 cgaactcggc gatgcgatgt tcctcggaca ggattttgag cttgcgcagc ggcaggcctc
       61 gggcaacggc ctggtcgatc acccaaggcg ctcgaagagc tccaacgtcc tggcctgcag
      121 gccaagcgct ttggtgctga cgttgtggtg cgcggccgcg tccacgcatc gcacatcgac
      181 gccgcgccgg cgtaactccg ccgcggcggc gagccccacc ggcccggcgc cgaccactaa
      241 gacgtcgcag tgatgcgcgt ccatcaccta cccccgtccg ggaacgctgg cagcacgtag
      301 cgggcgaaaa gccgcatctg ctcgagcatt tcgtcgactg tgttggccac gaacagcgtg
      361 gcgaagaagc cgtccagtcc ggccttctcg aacgcggcca ccttttcgca cacgtcgtcg
      421 ggcgttccga tcaagttgtc ggctacgtac ttgtcgagat cgacgccctt catcatggtg
      481 tccttaagcg aggtccggaa cagctcgaac gtcgacttct caagtcgctc gcgggcctgc
      541 tcggcggtgt cggccaggca caccaccgac tgcaagccga tggtgatcgt cgacgggtcg
      601 cggccggccg cggccgcgta gtcggccact ttcgctcggc cggaggcgat ttcggccggc
      661 ccgagcttgg cgggcagcca cccttcgcac cgctcgcccg cgcgttggag cgcgcccttc
      721 ccgtttccgc ccgagaagat cggcagcggc gactgaacgg gtttcgggta cgactcgaca
      781 tccaggaacc gccggtactt gccttcatac gtggaccgcg gcttttcgaa cagcacgcgg
      841 agcgcctcga tgccctcggc catcaagtgg gtccggttcg cgtcagccag atccgggacc
      901 acggcttcga actcgtcgcg gtaaccgccg accgccaccg ccaacgtgac ccgtccgccg
      961 ctgatgtggt cgagcgtggc cacctgcttg gccagcagca ccgggtcgcg catcggcagc
     1021 accagaattc cggtccctag ccgcaccacg gaggtcttgg ccgcgatcgt cgcgagcatc
     1081 atcagcggtt cgaaatagtc gggcgcggct ggccaggcct cgcgcacgaa tcgttgggtg
     1141 ctgaggtggt cgttgccggc gacctcgtga tacccgagct gctcggcctc gacgccgatc
     1201 cgcacgacgt cgtcggcggt ggcgaacgga acgggataga ccataccggc aagacacgtg
     1261 ggaacgtcaa ctccgaactt catgcctaca acgatgggcc ggtcacgggt ccgaccgaat
     1321 ctgtcgcgtg actgaattac gccaggttca gcgcggccaa ttcatagcgc ccgccgacgc
     1381 cgagcttcgg aaacgccttg tacaagtggt agccgacggt gcggggactg atgaacaact
     1441 gcgcgccgat gtcccggttg ctcatgccgg tcgccgccag gcgcaccacc tgcagttcct
     1501 gcggggtgag gtgtgccgcg ccgctggcct gctgtgcggc cgaggcgacg gtctctcccg
     1561 tcgcccgcag ctcagtacgg gcacgctcgg cccacgagtg cgcgccggtc tcctcgaaga
     1621 cgcgcaacgc gtcccgcagc ggctctcgcg cctcggatcg acgccggcac cggcgcagcc
     1681 attcgccgta gagcaactcg gtgcgagccc tggccatcgg ccggttcgtg tcgcggtaca
     1741 gccgcgccgc cgcccggtag tgctcctcgg cctcggcgtc agcgcccagc agcgcccggc
     1801 accgatgcag gttggcctgc accacgggat tctttcggtg cgacgcccac agctcgtacc
     1861 acgtcatgac ctccgcggct tgctcgggat ggccgctgcg cgccgcggcc tcggcccact
     1921 cgggcggcgc aaggtaggcc agggtcgggt ggcgttgcag ccggctgggc agcgcggtca
     1981 gtcgttgcag cgcctcggcg taccgcccgt agccgaggtc gagcacggcc accgccagcg
     2041 cggccagccc gacgccggag gactgccttt ccgatccgcg acgcatcgct tcggtggcca
     2101 gcaccacgca tcgttcctcg tcgccggtca tcgcggagat ccgagccaac acgccgcgca
     2161 ggttggctgc cgagtggtgc tgcccggtgt cctcggcgat ttgaattccc tcggtggccg
     2221 cacggagcgc ttcctgcagc cgccccgcca tcagggcggc ctgcgcgtgc tgaagcagga
     2281 tgtgcggcag cccgccgatc aggccgcgcg cgcgggcctc ggtcagcatc cgctccgtct
     2341 gagccgacgc ctcgtcggcg tcgccgatga ccatccgcaa gaaggcgctg tgaatgggca
     2401 ggtttccgat cgtcatcccc agctggtccg gtcggatcgc ccgaaccgcc ggcagcagcg
     2461 acgcaacctg gtcttcgggc ctgtccgact gcagcttggc gaggatggac gtcgcctgca
     2521 gcatcggccg cagcgggtga tcggcgggca gcaccaggcc gttcatgatt tcgatggccc
     2581 tgctgagatg cggcgcctcg tcggcgaagt aggccatccg gacgacttcg accagcatcc
     2641 tggcggcttc ttcgcgatcg gtgtggtaga cggtcaccgc gccgtcgagc aacatctcgg
     2701 aggcgtggcg cggggtcccc cgctcgaact ccacgcgggc cctgacccag gcgagttgcg
     2761 ccaacgtccg gcgatcctca gtcagcgtcg acgcctcctc ggccaggctc gcggcgcgat
     2821 ccagctgcgc cgaatcacgc gaattcatcg ccgcggccgc aagccgccga ccgcgtactg
     2881 ccgggtcggc cgtgaggcga gcagcgcgtt ccattgccgc ggcgctggcg gcgtatccgc
     2941 cgcgcagcac ggccctgtcg gcagccgcgg ccatcgcggc ggcggcctcg tcgtcgtaac
     3001 cggctgcggc tgcggctaga tgccacgcat gacgatcggc gtactcgggc tcatccaccg
     3061 cgtcggcgag cgcccgatgc acggccagcc gctcacccac ggacgatcgc gtgtataccg
     3121 cggaccgaat cagcggatgc cggaacgtga ttgactcgtt agtcagccgg attagctgtg
     3181 cacgctcggc aggctcgagg tcggccaacg agcggccgat gcggcccgcg gcggccagga
     3241 ccacgccgag ctcgccggtg tcctcggcgg cggccaccag cagcgcgaga cgcgtgtccg
     3301 cagccaaccc gtccagttgc gcgccgaacg cgtcgagcac tcggcgccct ccgatggaca
     3361 gaccctccgt cacggcgggc gggcccgcgg tcaccgattc tgctctgcgc agcgcggcgg
     3421 ccagttcgat cagcgccagc gggttgccgg tggcctcccc gatgagccga tcgcggacgc
     3481 ctgaggcgag gtcggcggcg cgctcagcga gcagcgcaga ggcggcggac tggctgagac
     3541 ctggcagccc caacaccgtc agtccgcgga aatcttcggc caccccgaag tctcggacgg
     3601 ccaacagcag cacgatgccc tctgcgccta ggcggcgccc agcaaacaac agcgtgtcga
     3661 tcgaggcccg gtccagccat tgggtgtcgt cgatcacgca cagcagcggc ccgtcggcgg
     3721 ccacctccga cagcagagtc aacgtggcca ggccggcgag gaaccggtcc acacccgggg
     3781 cctgcgccag accgaaggcc gcacgaagcg ccgccgcttg cggttcgggg agcgtgtcga
     3841 gccggtccag cacgtcgcgc agcaagaact ggagggcggc gaacggcagc tccgcctcgg
     3901 attccacacc cgcgcaccgc aatacctcaa agcctttggc gtgggccgcc acgtggtcga
     3961 gcagggccgt cttgccactg ccggcctcac cctggatcac taggaccgaa ctgtggcccg
     4021 accggccgcc attcaacagc cgttcaatgt cggcgagctc cgctgccctt ccgtgcagca
     4081 cggtttcaac ctatcggttc cgcgcctccg cttcacgttg cgagcgtgcg tgaatgccag
     4141 ccatctcggc gtgtcgttgg caagacgcgc gctcgcgcag acggcaacgg agcgctacag
     4201 cggcgcaccg ccgcgcaggt gctcgaagat cagcgacgtc tgcgtccccg cgacgtcagg
     4261 gtcggcattg aggttctcga cgacgaacgc ccgcaggtcg tcggtgtcgc gggcggcgac
     4321 gtgcaggatg aagtcgtcgg cgccagccag gaagtagacg tcgatcacct gcggcagctt
     4381 gcggatctct gagatgaagt ggcggatccg cccgcgggcc ttggactgca ggctcaccga
     4441 gatcattgcc tgcaacgggc ggccgatcgc ggcagggtcg acatcggtgt agaagccgcg
     4501 gatgacgccg atgtcctgga gtcgccgcaa ccggccgtgg caggtcgacg gcgcgatgcc
     4561 gaccatgtcg gcaagcgcag tgttgggaat ccgcgcgtcg tcgtgcaggg ctttgaggat
     4621 ccggcggtcg atgtcgtcga tctgcggcgg ccgaacatcc ttcgacggcc cagccgtgtt
     4681 cactactaat ttcgccgatc gttctgccat tcggctatcg tacagaattt tcatcgtgtt
     4741 gtttgtgtaa gagctgaagg ttcttcagaa tagaaccatc atcaccgatc aaggagcgat
     4801 catgcgcgtc ggagtcccga ccgagatcaa gaacaacgaa taccgggtcg ccatcacccc
     4861 cgctggtgtc tccgagctca cccgccgcgg gcacgacgtc gtcatccagg ccggcgcggg
     4921 cgaaggctcg gcgatctccg acaccgactt caaggcggcc ggagccgaga tcgtctccgg
     4981 tgccgagcac atctgggaac aggccgatct gctgctcaaa gtcaaggagc cgatcgagcc
     5041 cgagtatgcg ctgatgcgcc gcggccaaac gcttttcacg tacctgcacc tcgcggcgtc
     5101 gcagccctgc accgacgcgc tgatgcgctc gggcgtcact tctatcgcgt acgaaacggt
     5161 gcagacggca gacggagcgc tgccattgct ggcgccgatg agcgaggtcg ccggacggct
     5221 cgccgcacaa gtcggcgcat atcacttgat gaagcccgcg ggtggccgcg gcgtgctgat
     5281 gggcggcgtg cccggcgttg gtccggccga cgtcgtcgtg atcggcggcg gcacggctgg
     5341 ctacaacgcc gcccgggtcg ccagcggtat gggcgctcac gtcaccgtgc tcgacgtcag
     5401 cctcgccaag ctgcgcgagt tggacgccga gttcaacggc cggattcgta cccggcattc
     5461 gtcggcgctg gaactcgagg gcgccgtcaa acgcgccgac ctggtgattg gcgccgtgct
     5521 ggttcccggc gcgaaggctc ccaagctggt gtcgaattcg cttgtgtcgc agatgaagcc
     5581 gggcgctgtg ctggtcgaca tcgcgatcga ccagggcggc tgcttcgagg actcgcggcc
     5641 caccacccac gacgacccga cgttcccggt gcacggcacg gtgttctact gcgtggcgaa
     5701 catgccgggt gcggtgccgc ggacgtcgac gttcgcgctc accaacgcca ccatgccgta
     5761 cgtgatcgcg ctggccgacc gcggctggca ggccgcatgc cgagccgatt cggctctggc
     5821 cgaagggctt tcgacgcacg agggcgctct gctcaacgat caggtggccc acgacttggg
     5881 cctgccgttc accgacccgg cgaaggttct ggcctagcag ttcagaacag cggttcggtg
     5941 cgcagtccgc aatgcgcact gatgacctcg aaatcgcgat cccgatgcgc cacggcttcc
     6001 ccaagccgca tcgccatggc ggccacttga caatcaatca ggctgcgcac cgtgtgtccg
     6061 cgtcggcggc agcgccgatg gatggccgcg gcgtcttcgg cgtcacgcaa cggggccagc
     6121 ggctcgatgt cgaaacgctg tgggagacgg cgtcgcatcg cagccttgga ctcatcggtt
     6181 gtcccgatca gcaattccat caacaccacc tccggcacga tgaccgtcgc gccaacggcg
     6241 atacggtccg ccagccagcg gccggccgtc gactggggtg cgacgaaata gtcgatccac
     6301 accgaggtgt cgacgatcat ctacgactca gtgccacgcc ggggttcggc gtcggaaaac
     6361 gactcgatct cgtcgttcgt gtaatcaaag ccgctgccct gcatcgccaa cgcttcgtcg
     6421 cgagtgaggg cttcacccac gagcctgcgc agtgccaggt cgaccgcact tcgtttggaa
     6481 tcgagccggt acattcgctg cgccgctgcc acgagctcgt cgtcgatttc gatattcgta
     6541 cgcgacattc tccgaagcat acaccccaat gtgtatgaag gtgttgccgc agctgcctaa
     6601 ctgagcgtcg acacgatctg cttcgtcaac tcgccgatca gcggccgcag gttctccgcc
     6661 ttcgggtcat cggacgcggt ccgagtcatc atcgccagca gcaccttccg gccgtccggc
     6721 ccgtacgcga tgccgacgtc gttggtgctg gcgtagtcgc cgctgccggt cttgtcggcg
     6781 ctggtccagc cctgcggcag gccggctctc atgctcgacg tcacattggc ccgcatccac
     6841 tcgtcgagtt gctgccggct tgggacggcc agtgcggagc cggtgagcag gttctgatag
     6901 ccgcgcgcga acgcccgcgg tgtcgtggtg tcgcgcggat cacccggcag tgccgtattg
     6961 agctcgagct cccagcggtc gagccgggtc ttgtcatcgc cgatgctgcg agcgaacgcg
     7021 gtgatctccg acggcccgcc gatcgtggtc agcagcaggt tggctgcgca gttgtcgctg
     7081 cgttgcagcg cggcagcgca cagatcgctg agcgtcatgg cgtgaccggc gtttgattcg
     7141 gtcacgggcg aattgggaag aatagccaca ggattgacga acacctggtc gctgagatga
     7201 agttcgccgc ggtcgcagcg ctgcagcacg ctcgcacccg cgtaggtctt gaacgtcgag
     7261 cacatcgcga acatgtcgtc ggcccgaaac tccgccgtgc gccccgaccc cagatcggcg
     7321 gcgaacagtc cgacgcgggc gttgtgccga cgttccagcg cagtgatccg ctcgtccagc
     7381 ggcggcatcg gctcggccgg gtccttcaga gtcggtttcg cagtgcacgc cgccgcgaac
     7441 agcgtcgcac cgaccacaat gatccggcgc cgcgacagct cacccatcgg cggtcaagcg
     7501 gcgcctatag cgccgcatcc cggcgatcca cgcgtcgtag tcggcgccct tctgccggta
     7561 catgtcgatc acctccgcgt gcggcaacac caaaaatcgg ccctgccgca ctgcctcgac
     7621 agtcacgtcg gcgacccgcg cgggttccag cacctcgccc gcgctgatga ccgatcggcc
     7681 cgccaactgc cctgcgggat ctgcggattc gcgcagttcg gtcagcagct gggtgtcgac
     7741 gcccatcgga cagacgcagc tgacgccgac gccgtcgtcg ccgtaagtga tcgccagcca
     7801 ctccgcgaag ccgagcgcgg catgcttgga caccgcgtaa cccgcggccc cgagttgggt
     7861 cagcagtccc gccgcggatg ccacggacac gaagtatccg ctgccgcgct cgacccactg
     7921 cggcagcagt tgcgtcgcgg cccggatgtg cgcgcgcaga ttcacgtcga gcacccgatc
     7981 ccagtcagca tcggacatgc ccagccccgg cgggccgccg atgccggcat tggccacgta
     8041 aatgtccact gacccgaatt ctgttgccgc caacgcgatc agcgcggcga tgtccgcttc
     8101 ggcgctggcg tcggcctgac gtggcaccgc gtgccccgaa atcatgctgg cggtctgctg
     8161 cgcggcggcc gcgtcgatgt cgccgaccac gacgcgtgca cctgcctcgg ccagcgcggt
     8221 ggccagcgca cgaccgatgc ccgagcctgc gccggtgacg atcgcaaccc tgccttcaat
     8281 gtccatggtt gccctacagc ttgcgaagtg gttgcgggag ttgacgtttg gcctggtacg
     8341 gcccgagcac cgccgcgccg tacggcccgg tcagaactcg tcgcgccacc gcgttcacct
     8401 cgtcgagggt gacgctgtcg atctgggcca gcgtgtgtcc gatgctgcga tgctcaccgt
     8461 agttgagttc gctgcggccg atccggttca tccgggacgc cgaatcctcg agtcccagaa
     8521 cgaggccgcc gcgcagtgag cccttggcga tccggcactc gtcggcggtg atgccgtcgc
     8581 gggcaacggt ttccagaact gacgtggtga cgccgacgac gtcgccgaac cgctccggca
     8641 ggcacgccgt gtacaccgac agcgcgccgc tctcggcgaa cgtgtcgacc gtcgaataca
     8701 ccgtgtacgc cagcccccgc tgttcgcgga tctgttggaa cagccgcgag ctgagcccgc
     8761 cgccgatcgc ggtgttcagc accgccagcg cccagcggtg ctcccagtgc cgtccgggac
     8821 aacggacccc aagggtcatg tgggtctgct cggcgtctcg cctgcacagt gtcagcgccg
     8881 gccggcccgc gactcggcct gcgcccttgc gcggcggcac cggcatgcgg ccacgctcca
     8941 gccgtgctcc gaagtagcgc cgaaccagcg acaccacctg atcgtggtcg acgttgccgg
     9001 ccaccgcgac gaccatccgc tccggggtgt agcgccgtac atggaaggag tgcagctgcg
     9061 cgcgcgtcat cgaagacacc gagtcgacgg tgccgaccac cggacggccg accgggtgcc
     9121 gaccgaacag cgccgccagc aacacgtcgc cgatcgcgtc ctccgggtca tcgtcgcgca
     9181 tcgcgatctc ttcgagcacc acgtcgcgtt ccagttcgac gtcgtcgggc gcgcacaccc
     9241 cgttgagcac cacgtcggcc accagctcga cggccagctc caggtcgctg tccagcacgt
     9301 gggcgtagta gcaggtgtgc tctttggcgg tgaacgcgtt cagctcaccg ccgaccgcgt
     9361 cgacggcctg ggcgatctgc accgccgtgc gcgtgggcgt cgccttgaac agcaggtgct
     9421 cgaggaagtg cgcggcgcca gcgacgctcg ctccctcgtc gcgggacccc actcccaccc
     9481 agacgccgac cgacgccgag tgcacgccgg gcaggcattc ggtgacgacg cgcaagcccc
     9541 cgggaagcgt ggtgcgccgc aggccggcgc caacgtcagg cgccggtatg cgcggcatcc
     9601 gcaggtgcgg gggcgtccgc tgcttcctct tcggccacca ggaccagcga gatcttgccc
     9661 cggttgtcga tgtcggcgat ctccacgcgc agcttgtcgc cgaccttgac gacgtcctcg
     9721 accttggcga tccgcttgcc cctgccgagc ttggagatgt gcaccagccc gtcgcggccg
     9781 ggcagcagcg acacgaacgc gccgaagtcg gtggtcttga cgaccgtgcc gaggaaccgc
     9841 tcgcccacct tgggcagctg cgggttggcg atcgcgttga tcttgtcgat cgcggcctgc
     9901 gccgacaggc cgtcggtggc gccgacgaac acggtgccgt cgtcctcgat tgagatctgc
     9961 gcgccggtct cctcggtgat cgaattgatc atcttgccct tgggcccgat cacctcgccg
    10021 atcttgtcca ccggcacctt catcgtggta acgcgcggtg cgtaggggct catctcgtcg
    10081 ggtgcgtcga tggcttcggc catcacctcg aggatggtca gccgcgcgtc cttggcctgc
    10141 gacagcgcgt tggccagcac ctgcgacggg atgccggcga gcttggtgtc cagctgcagc
    10201 gcggtgacga agtccttggt gccggccccc ttgaagtcca tgtcgccgaa cgcgtcctcg
    10261 gcgccgagga tgtcggtcag cgctacgaac cgccgctcgg tgccgccgtc ctccagctcg
    10321 acgtcgtcgg aaaccaggcc catcgcgatg cccgcgaccg gcgccttcag cggcacaccg
    10381 gcgttgagca ggcccagggt ggacgcgcag accgagccca tcgaggtcga gccgttggag
    10441 cccagcgcct cggacacctg gcggatcgcg tacgggaatt cgtcacgatt cggcagcacc
    10501 ggcacgagcg cgcgctcggc gagtgcgccg tgcccgatct cgcgccgctt cggcgagccc
    10561 acgcggccgg tct
//
LOCUS       DASWBF010000015         3486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_461940,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000015 DASWBF010000000
VERSION     DASWBF010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461940"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..973
                     /locus_tag="VF874_00415"
                     /pseudo
     CDS             125..973
                     /locus_tag="VF874_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
     gene            complement(1103..2749)
                     /locus_tag="VF874_00420"
     CDS             complement(1103..2749)
                     /locus_tag="VF874_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX9174606.1"
                     /translation="MHKQLDELLAARDQMEALLRVVVEIGSDLDLDATLHRVITAAMT
                     LTGARYGALGVHGADSTLVSFLHDGIDDETVQRIGHLPAGKGVLGVLLERPETLRCDD
                     LTAHPAAVGFPEHHPRMRAFLGVPIRVRGDVFGSLYLTDDRPAKQFTDSDEIAARALA
                     SAAGVAIDNAKLFERVCASAKWTEASREITTALLSGEAHVNPLQLIAERARELTEAEQ
                     AIVLVASDDGVPSEHVDTLVISTAVGLHSADVLGQTVPVEGSTTGSVYRSGTPVITDS
                     FRRPIQAFTDVGQRPAIVMPLSAGDAVIGVMAVARNAHQQPFGHSHLELMSDFADHAA
                     VALTLAASREQAREVSILADRERIAHDLHDHVIQRLFAAGMDLQGSIARARSSELVAR
                     LSQTVDDLQTTINDIRSTIFKLQPPAGHSGGFQQRMQRAVRDLTENRDIATSLYISGP
                     MSVVGSDLAEQAEAVVIEAVSNTLRHSGASSLIVEIAVADDLTVQIVDDGCGIPADNK
                     RRSGLVNLQRRAEQVGGRCRITSPPAGGTRVHWTAPLIGL"
     gene            complement(2974..3315)
                     /locus_tag="VF874_00425"
                     /pseudo
     CDS             complement(2974..3315)
                     /locus_tag="VF874_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          583 a         1112 c         1175 g          616 t
ORIGIN      
        1 tggttgtctc gcccactgtg cacgcagctg attctcaaga tcgctgcatt tcccatcggt
       61 agtcgcggcg cacgatctga gccgcagcgg atactcacgc ccgccgcccg accccgctca
      121 gcatctgacg cataccgacc cggaccgccg cgatttcatg ctcagctgcg gtgcggcgct
      181 gcatcactgc gtaatcggcc tggcggcatc gggatggcag gcgacggtgc acagattgcc
      241 caatcccgcc cagccggatc acctcgcggc gctcgaggtt caccagcacc gagccggcca
      301 gctggacatt cagctcgcgg cagcaatccc gcagcggcgc accgatcggc gccattacag
      361 ctggtggccg gtgtcagtcg cggacctgca ggtgatcagt gcccgcgccg cacgtgccgg
      421 gctgatcctg cgccggctag agtcgcttcc ggcgttgaga tatcttgtgg cacaagcggc
      481 ttggcgacac gcgaccgatg ccgattatca gtctgaactg agggcgtgga gcggtcggca
      541 cgccgcgcca gccggtgtgc cggcccgcaa caccccggag cccgacccgg cggcgccgat
      601 cccggcgcgg cggttcgccg aagcggcgct ggcgcagccg gccagcgccg aggccgagga
      661 cgataacgcg gtggtgctcg cgctgggcac cgcccaagac actgctctgg cgcggttgtg
      721 cgctggtgag gcgatcagca tggtgctgct gaccgcgacg gcgatgggct tggcaagctg
      781 cccgatcacc gagccgctgg aagtccccga aacacgagat gcggtgcgag acgatgtctt
      841 tggcggcaac ggatttccgc agatgctggt tcgcatcggc tgggcgccga taggagccga
      901 cccgctgccc gccgctcctc gccggttgct cagcgaggtg gccgagtggt tggacggttc
      961 accgttggcc tgatctcggg atgcgtctga catcagcgca gccgactccg acggctcagt
     1021 gagcggatcc aacaggacct acgctccaca ttgcccgatg tgaggcgagc gtttctcaac
     1081 ggaggtgagc gtgctcggct ctttagaggc cgatcagagg cgcagtccag tggactcggg
     1141 tgccgccggc cggcggtgag gtgattcgac aacggccgcc gacctgttcg gcacgtcgct
     1201 gcaagttaac aagtccgctg cggcgcttgt tgtcggcggg gatgccgcac ccgtcgtcga
     1261 cgatctggac ggtcaggtcg tcggcgacag cgatctcgac gatcaggctg ctggccccgg
     1321 agtgccgcag ggtattgctg acggcttcta tcacgacggc ctcggcctgc tcagcgagat
     1381 cgctgccgac caccgacatg ggaccggaga tgtacagaga tgtcgcgatg tcgcggtttt
     1441 cggttaggtc gcggactgcg cgttgcatcc gctgctggaa gccgccgcta tggccggcag
     1501 gtggttgcag cttgaagatc gtcgagcgga tgtcgttgat cgtggtctgc aagtcgtcga
     1561 cggtctggct gagtcgagcc acgagttccg atgagcgggc gcgggcgatg ctgccctgta
     1621 ggtccatccc ggcggcgaac aacctctgga tgacgtggtc gtgcagatca tgcgcgatgc
     1681 gttcgcggtc ggcgaggatg ctcacttcgc gggcctgttc acggctggcc gccagcgtca
     1741 acgccaccgc tgcgtggtcg gcgaagtcgc tcatcagttc cagatggctg tggccgaacg
     1801 gctgctggtg ggcgttgcgg gccactgcca tgacgccgat cacggcgtca ccggcggaca
     1861 gcggcatcac gatggccggg cgttgtccga cgtcggtgaa cgcctggatg gggcggcgaa
     1921 acgagtccgt gatgaccggc gtgccggacc ggtacacact cccggtggta gatccctcca
     1981 ctggcactgt ttgtccgagc acgtcggcgg agtgcaatcc gaccgcggtg gagatcacca
     2041 gcgtgtcgac gtgctcactg ggcaccccgt cgtcgctggc aaccaagacg atggcctgtt
     2101 cggcctcggt cagctcccgg gcccgctcgg cgatcaactg caacgggttc acgtgcgcct
     2161 cgccggacag cagtgccgtg gtgatctcgc ggctggcctc ggtccacttc gccgacgcgc
     2221 atacccgctc aaagagcttc gcgttgtcga tggccacgcc ggcggcggag gccaatgctc
     2281 gggcggcgat ctcatcggag tcggtgaatt gtttcgcggg cctgtcatcg gtcagataca
     2341 agctgccgaa cacgtcgccg cgtacccgga tcggcacacc gaggaaggca cgcatccgtg
     2401 gatgatgctc cgggaatcca acagcagccg gatgtgcggt caggtcatcg cagcgcagcg
     2461 tttccgggcg ctccagcaac acaccgagga cccctttacc ggctggcaga tgaccgatcc
     2521 gctggactgt ctcgtcatcg atcccgtcgt gcagaaacga aaccagggtg ctgtcggcac
     2581 cgtgtactcc cagggcaccg tagcgggcgc cggtaagcgt catcgccgcg gtgatgacgc
     2641 ggtgcagtgt tgcgtccaga tccagatcgg agccgatctc gaccacgacc cgcagcagcg
     2701 cttccatctg atcgcgagcc gcaagcagct catcgagctg cttgtgcatc cggccgacca
     2761 gcccgcgctg tcccagtcgt gagaatgccg aatcgtcgtc agagatgttg aaccacccca
     2821 tagctctcga catcctttgc ggccagcaca actttacgcc gccaagctac gaggccgccc
     2881 ctccggccgc ggcatcgtca agttattggg atgcttgtgg cgcagtcgat gtgagtggac
     2941 cggctggcga aagatggtgg ctgactgcgt ctttcaagcc gctgcggcgg taatgatctg
     3001 gttccagacc gcgaagcggc tggtcatctc ggttcgccat tcgggcgcgc cgatcagatg
     3061 acgacggggc ccaaagtgct gtcggatgct gccgaacgca aacagcaacc gttgggcctg
     3121 cccaggcgag gcgaagcgtt tcatcacccg ttcccggacc ctgctcgact gatgggagtt
     3181 ctcggcccgg ttgttgaggt acttcgagcg gcgatgggtc accgacggca acagctcgcg
     3241 gtgagtgagc tgatagctgg ccagcttgtc ggtgaccagc acccgcggca cgtatcgcag
     3301 gcccttgagc agcttagtag ggtcgagagc tggcgcggta acgagcggca agcatcacga
     3361 aaagtgcccg cgaaacgccc tcggtgcatc acgtaggaat gcccgcgaaa cggtgatcct
     3421 cgccaggcat ggaagagtgg gctgtcgatg tcgtctcgtg ccgaggtcac cgcgaagttc
     3481 gccaag
//
LOCUS       DASWBF010000016         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_198535,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000016 DASWBF010000000
VERSION     DASWBF010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198535"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VF874_00430"
     CDS             <1..277
                     /locus_tag="VF874_00430"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729628.1"
                     /note="mycothiol biosynthesis; catalyzes the ligation of
                     cysteine to a mycothiol precursor; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HEX9174607.1"
                     /translation="YRSDREWSADVLGGAQARLARWRQAVALPSGPDAADVLARVRRY
                     LADDLDTPKALAALDGWATDAVEYGGGDATAPALVRACVDALLGVEL"
     gene            274..531
                     /locus_tag="VF874_00435"
                     /pseudo
     CDS             274..531
                     /locus_tag="VF874_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
     gene            585..1850
                     /locus_tag="VF874_00440"
     CDS             585..1850
                     /locus_tag="VF874_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX9174608.1"
                     /translation="MVDNYRVMSSYFVDNDDSEYKGGWNEIHNTARVYTPADTAIQTP
                     NSDTPYSAVGADLRTEPLVLTVPPIEQHRYYSLQFVDGYTANFAYVGSRTTGNSGGKY
                     LLAGPNWTDEKPAGIDQVIVSDTELAFVLYRTQLFGPDDLDNVKTIQAGYRVQPLSAY
                     LNEPAPAPAPPIDFVPPLTPEQQKTSPQFFEILDFVLRFAPTPPSERELRARFATIGI
                     GPDGAFDADTLTPEMRSAIEGGIADAWAEFDALKKDKVDTGQVSSADFFGTAADLKGN
                     YLYRMAGAALGIYGNTAAEAIYPVSFSDSTGAPLTGANNYTYRFPPGQLPPVNAFWSL
                     TMYELPKSLLVANPINRYLVNSPMLPGLVKDPDDGYTIFIQHHSPGAEQQANWLPAPP
                     GPFVVVLRLYWPKPEALSGQWTAPEPVKV"
     gene            complement(1847..>1989)
                     /locus_tag="VF874_00445"
     CDS             complement(1847..>1989)
                     /locus_tag="VF874_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9174609.1"
                     /translation="AEYVAQLEERGDAEVDMTEALSKVDGDALAAEFERYLRRRGPGF
                     RG"
BASE COUNT          336 a          685 c          641 g          327 t
ORIGIN      
        1 ttaccgttcc gaccgggagt ggagtgccga cgtcctcggc ggggcgcagg cgaggctggc
       61 gcgctggcgc caggccgtcg cgctgccgtc gggcccggat gctgccgacg ttctcgcacg
      121 cgttcgccgc tacctcgccg acgatctgga tacgccgaaa gcccttgctg cccttgatgg
      181 ttgggctacc gacgcggtgg agtacggcgg tggtgacgcg actgcgcccg cactggtgcg
      241 cgcttgcgtc gatgcgctgc ttggtgtcga gttgtgaaga agtcgacgtc ggctcagaag
      301 tgcgccgacg tcttcgtcaa ggggatcgaa ggccgtaaac gtcgggtgaa ctgtccgggt
      361 tgggtcggtg tgttccgctg gctgaaacca ctcttgacca cctccgccgg cgaggcgcag
      421 ctggccaaga cggtcccgga actgctgccg cgcatggacg ccgagatcgc cgcgctcggg
      481 cggtcgatga gcgcgcgcac cgaagcgctg gagaagcgga cgacgaggtg atccccgagc
      541 aggtccgcgc gatcgcgaaa gacgcctaca tctacggctt tccgatggtg gacaactacc
      601 gggtcatgtc ctcctacttc gtcgacaacg acgattccga atacaagggc ggctggaacg
      661 agatccacaa cactgcccgg gtgtacacgc cggccgacac ggcgatccag acgccgaatt
      721 cggacacccc gtactccgcg gtcggcgcgg acctgcgcac cgagccgttg gtgctcaccg
      781 tcccgccgat cgagcagcac cgctactact cgttgcagtt cgtcgacggc tacaccgcca
      841 acttcgccta cgtcggcagt cgcaccaccg gcaacagtgg cgggaaatac cttctggcgg
      901 gcccgaattg gacagacgag aagccggccg ggatcgacca ggtaatcgtt tccgacaccg
      961 agctcgcatt cgtgctgtac cgcacgcagc tgttcgggcc cgacgacctc gacaacgtca
     1021 agacgattca ggccggctac cgggtgcagc cgctgtcggc ctacctcaac gagccggcgc
     1081 ctgcgcccgc gccaccgatc gactttgtgc cgccgctgac acccgagcag cagaagacct
     1141 cgccgcagtt cttcgagatc ctcgacttcg tgctgcgatt cgcccctaca ccgccatcgg
     1201 agcgggagct ccgcgcgcgg ttcgcgacga tcgggatcgg tccggacggc gccttcgacg
     1261 ccgacacgtt gactcccgaa atgcggtccg cgatcgaggg gggaatcgcc gacgcctggg
     1321 ccgagttcga cgcgctgaag aaggacaagg tcgacaccgg ccaggtcagc tctgccgact
     1381 tcttcggcac cgcagccgat ttgaagggca actacctgta ccgaatggcc ggcgccgcgc
     1441 tcggcatcta cggcaacacc gccgcggagg ccatctatcc ggtttcgttc agcgactcca
     1501 ccggtgcgcc gctcaccggc gcgaacaact acacctatcg ttttccgccc gggcagctgc
     1561 ccccggtgaa cgcgttctgg tcgctgacca tgtacgagct accgaagagc ctgttggtgg
     1621 ccaacccgat caaccggtac ctggtcaact cgccgatgct gcccggcttg gtcaaagacc
     1681 cggacgatgg ctacacgatc ttcatccagc accactcgcc cggcgctgag cagcaggcca
     1741 actggctgcc cgcgccgccg gggccgttcg tcgtggtgct gcggttgtac tggcccaagc
     1801 ccgaggcgct cagcggtcag tggacggcac ctgagccggt caaggtctag cctctgaagc
     1861 ccgggccgcg gcggcgcagg tagcgctcga actccgcggc cagcgcgtcg ccgtcgacct
     1921 tggacagcgc ctcggtcatg tcgacctcag cgtcgccgcg ctcttcgagc tgagcgacgt
     1981 actcggcga
//
LOCUS       DASWBF010000017         8972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_396444,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000017 DASWBF010000000
VERSION     DASWBF010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396444"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VF874_00450"
     CDS             complement(<1..354)
                     /locus_tag="VF874_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX9174610.1"
                     /translation="MPVRSALRSGVLSPQRAVPSSIPRPEYAWKPTVHEGSEPWVQTP
                     EMIENMRVAGRIAAGALAEAGKAVAPGVTTDELDRIAHEYMVDHGAYPSTLGYKGFPK
                     ACCTSLNEIICHGIPD"
     gene            complement(364..960)
                     /locus_tag="VF874_00455"
     CDS             complement(364..960)
                     /locus_tag="VF874_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX9174611.1"
                     /translation="MTGIDQDTSALRISDADRNGTLRRLHNAVALGLIDIDEFEDRSS
                     RVSQARTRGELDTLVDDLPGPGAFVTSVADRVELRGWMGSLKRHGEWTVPTRLALVRR
                     VGSVELDLTNARFAGPVVVIELDMIRGSVDIRLPEGASASIDDVTVYGGSARDLRKEP
                     PAEGRPHVVLTGRVVMGSVKIRGPRRQKFGRLRAIGPR"
     gene            complement(1019..1402)
                     /locus_tag="VF874_00460"
     CDS             complement(1019..1402)
                     /locus_tag="VF874_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX9174612.1"
                     /translation="MTEYLDLDDLLDIVREVVGGDAVVGDYGLLESALARPRASVFGQ
                     DAYPDLHLKAAALLHSLARNHALLDGNKRLAWTACRTFLAINGQWISAPEDERFEFVI
                     RVATGVLPDLGKVAEQLRTWSYPEG"
     gene            complement(1399..1605)
                     /locus_tag="VF874_00465"
     CDS             complement(1399..1605)
                     /locus_tag="VF874_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX9174613.1"
                     /translation="MGMTLRTSAQQTEALRQQAAAEGRSMQAVALSAIDEYIARRTHK
                     AKVAAALQRVVREEAGVLERLKDA"
     gene            complement(1682..3481)
                     /locus_tag="VF874_00470"
     CDS             complement(1682..3481)
                     /locus_tag="VF874_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9174614.1"
                     /translation="MASRVTHLLAVALVVAGVLAGCTPRPDGPTPVAEQFFAALATGN
                     TSAAAALADRPDDARAALNAAWAGLQATRLDAQVLSSSFDEDTGTVDYRYTWHLPKNR
                     TWTYDGRMSMIREEGRWEVRWTPAGLHPKLGEHQTFALRADPPQRASVNEVGGTDVLK
                     PGYLYHYELNATQAGTQLMPTARAVVEALRPFDDTLDPQRLAEQASSSTTPVDLITLR
                     PDDNNRVAPAIGQLPGVVVTPQADMLPTDDGFAPALVAQVKKSVIDQLDGEAGWRVVS
                     VNQNGVDVGILNEVPGTPAPSVSMTLDRSVQNAAQGAVANVGRQAMIVAIRPSTGAIL
                     AIAQNASADAEGPLATTGLFPPGSTFKIITAGAAIQRDMATPNTLLGCPGTADIGHRT
                     IPNYGGFDLGVVPMSRAFASSCNTTFAELASRMPPTALTVAAAQYGIGVDYNVAGVPT
                     VTGSVPPTVNLAERTEDGFGQGKVLVSPFGMALAAATVAAGKTPVPQLILGRPTAIAG
                     ERTDITGQMVDSLRPMMRLVVTNGTAKDIADSGEIYGKTGEAEFQGGSHSWFAGYRGD
                     MAFAALIVGGGSSEYAVRMVRYMLAQLPPDYLG"
     gene            complement(3597..4451)
                     /locus_tag="VF874_00475"
     CDS             complement(3597..4451)
                     /locus_tag="VF874_00475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9174615.1"
                     /translation="MSAPPVFRLVDERRVSVARDPTAVQRVIDADPIGSCMVAARVAE
                     HGIEPSAIGGELWTRRGADESLCYAGANLIPLRGAAADLHAFADKAMGVARRCSSVVG
                     RAELVLPMWRRLEHTWGPARDVREQQPLMAMASRPSSPIDPGVRRVRVDELEPYLVAA
                     IDMFIGEVGIDPRLGDGGRGYRRRVAGLIAAGRAWARFEHGKVIFKAEVGSQSPAVGQ
                     IQGVWVHPEYRGHGLGTAGTAAVVASIIDSGRMASLYVNDFNTIARAAYTRVGFAEVG
                     TFATVLMD"
     gene            complement(4479..5627)
                     /gene="ispG"
                     /locus_tag="VF874_00480"
                     /gene_synonym="gcpE"
     CDS             complement(4479..5627)
                     /gene="ispG"
                     /locus_tag="VF874_00480"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX9174616.1"
                     /translation="MTVGLGLPTPPAPTLAPRRKTRQLMVGDVGIGSDHPIAVQSMTT
                     TKTHDVNATLQQIAELTATGCDIVRVACPRQEDADALAEIARHSQIPVIADIHFQPKY
                     IFAAIDAGCAAVRVNPGNIKEFDGRVAEVTKAAGAAGIPIRIGVNAGSLDKRFMEKYG
                     KATPEALVESALWEASLFEEHGFGDIKISVKHNDPVVMVAAYELLASQCEYPLHLGVT
                     EAGPAFQGTIKSAVAFGALLSRGIGDTIRVSLSAPPVEEVKVGNQILESLNLRPRSLE
                     IVSCPSCGRAQVDVYTLANEVTAGLEGLDVPLRVAVMGCVVNGPGEAREADLGVASGN
                     GKGQIFVKGEVIKTVPESQIVETLIEEAMRIAEERGSDTSGSPVVTVS"
     gene            complement(5636..6859)
                     /locus_tag="VF874_00485"
     CDS             complement(5636..6859)
                     /locus_tag="VF874_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX9174617.1"
                     /translation="MKFALGLILLALLIIVSVALHECGHMWAARATGMKVRRYFVGFG
                     PTLWSTRRPNKLGSTEYGVKAIPLGGFCDIAGMTSVEELKPDEVPYAMYRQKVWKRVA
                     VLFAGPGMNFVVGLVLIYAIAIVWGLPNLNPDTRAVIGETCSVTPQLAKDQQPKCDGS
                     GARPAAEAGIRPGDVIVKVGNKDVHNFDEVVSAVRPLTGPTPVDVERDGQKITFTVNV
                     TQTQRFTSENPNTASTVGAIGASSALPGPTQYNPLTAVPATFTFTGDLSVLLGKALVT
                     IPTKVGALVHSIGGGERDPETPISVVGASIIGGDAVDQDLWVLFWFLLAQLNFVLGAV
                     NLLPLLPFDGGHIAVAVFEKLRNMIRSARGMAAAAPINYLKLMPATYVVLIVVVGYML
                     LTVTADVVNPLRPFQ"
     gene            complement(6856..8025)
                     /gene="dxr"
                     /locus_tag="VF874_00490"
     CDS             complement(6856..8025)
                     /gene="dxr"
                     /locus_tag="VF874_00490"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX9174618.1"
                     /translation="MSNRIRVLILGSTGSIGTQALEVIAANPDRFEVVGLAAGGANAQ
                     LLADQRDATGVTNVAVADESAADAVGAIHSGPDAATELVRNTEADVVLNALVGALGLK
                     PTLAALDSGARLALANKESLVAGGPLVLKAAKPGQIVPVDSEHSALAQCLRAGEANEV
                     AKLVLTASGGPFRGWSAADLESVTPEQAGAHPTWNMGPMNTLNSASLVNKGLELIETH
                     LLFGVPYEDIEVVVHPQSIVHSMVTFTDGSTIAQASPPDMKLPISLALGWPDRVPRVS
                     AACDFATAASWDFEPLDAAVFPAVDLARQAGRLGGCMTAVYNAANEEAAAAFLTGRIR
                     FPAIVRTIADVLGAADQWAAEPATVEDVLDAQRWARARANSAVSRAVAQEVIPAR"
     gene            complement(8069..8884)
                     /locus_tag="VF874_00495"
     CDS             complement(8069..8884)
                     /locus_tag="VF874_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174619.1"
                     /translation="MINLQNIDSTVKAIYGALEPWVRYGFQLAEYAVGWIPGVGWLAP
                     QVMYFYNLGERIVSSAIFNTTQWLTGQGNFIQNLGQFGVDTVNSFIFFANDQLAFWLP
                     PLPPIPPLPGGSNTFTILDAPALPQTVQTVQKRLTAPVDLAGTVFGLPGNLDTSRLTA
                     ARVVDAQVTNLKLAAKPSPAIDTSNPLRKVDPFNITTNAQGTRKSLLGAFDVTNTPVG
                     TTNPSAGATIPSAASPTRGRLADAIKGRLSDAFKVRLADKPADDAANAVGPAK"
BASE COUNT         1392 a         3076 c         3016 g         1488 t
ORIGIN      
        1 gtccgggatg ccgtggcaga tgatctcgtt cagcgacgtg cagcacgcct tcggaaaacc
       61 cttgtagccc aacgtcgatg ggtacgcccc gtgatcaacc atgtactcgt gcgcgatgcg
      121 gtccagttcg tcggtggtga caccgggtgc gaccgccttg ccagcctcgg ccaacgcgcc
      181 ggcggcgatg cggccggcga cgcgcatgtt ctcgatcatc tcaggggtct gtacccacgg
      241 ctcgctgccc tcgtgcactg tcggcttcca ggcgtattcg gggcgcggga tcgacgacgg
      301 caccgcgcgc tgaggtgaca gcacgccgga gcgcagggcg gaacggacag gcatggccac
      361 agcctagcga ggcccgatcg cccgaagcct gccgaacttc tgtcgccgcg gcccgcggat
      421 cttcaccgag cccatcacca cgcggccggt cagcaccacg tgcggcctgc cctcggcggg
      481 tggctccttg cgcagatcgc gcgcgctgcc gccgtacacc gtcacgtcgt cgatcgacgc
      541 gctggcaccc tccggcagcc ggatgtccac cgagccgcgg atcatgtcca gctcgatcac
      601 cacgacgggc ccggcgaagc gggcgttggt caggtccagt tcgaccgagc cgacgcggcg
      661 caccagcgcc aaccgggtgg gcaccgtcca ctcaccgtgc cgtttgagcg aacccatcca
      721 gccgcgcagt tccacgcggt cggccaccga ggtgacgaac gcgccgggac cgggcaggtc
      781 gtcgacgaga gtgtccagct cgccgcgggt gcgggcctgc gacacccggc tggagcggtc
      841 ctcgaactcg tcgatgtcga tcagcccaag cgcaaccgcg ttgtgcagcc gccgcagcgt
      901 gccgttgcgg tccgcgtcgc tgatgcgcag cgccgacgtg tcctgatcga ttcctgtcat
      961 ggccttacca acttaccgcc gcgctccacg cgataaatcc gcatgctggc gcagcggctc
     1021 agccctccgg gtagctccag gtgcgtagtt gctcggcaac cttgccgaga tcgggcagta
     1081 caccggtggc gacccggatg acgaattcga agcgttcatc ctcgggggcg ctaatccact
     1141 ggccgttgat ggcgaggaac gtccggcagg ctgtccacgc cagtcgcttg tttccgtcca
     1201 gcagagcgtg gttccgcgcc agggagtgca gcagcgcagc ggccttgagg tgcagatctg
     1261 ggtaggcatc ctggccgaac accgacgcgc gggggcgcgc cagagccgat tcaagcaagc
     1321 catagtcccc gaccacggcg tccccgccga cgacctctcg gacgatgtcg agcaggtcgt
     1381 cgagatccag gtactcggtc atgcgtcctt aaggcgctcc agaaccccag cctcctcgcg
     1441 caccacgcgc tgcagcgccg cggcaacctt ggccttgtgc gtccgccgtg cgatgtactc
     1501 gtcgatggcc gacagcgcga cggcctgcat cgaccgcccc tcggcggcgg cctgctggcg
     1561 cagcgcttct gtctgctgag cactggtgcg cagagtcatt cccatgaccc agcatgatac
     1621 ctgattgata ccacccgctt gagtcatcgc aggcgcgcat cccgggttgt cggtcggcaa
     1681 cctagccgag gtagtccggc ggcagctgcg ccaacatgta ccggaccatc cgcaccgcgt
     1741 actcggagct tcctcccccg acgatcagag ccgcgaacgc catgtcgccg cggtagcccg
     1801 cgaaccacga gtgcgagccg ccctggaact ccgcctcccc cgtcttgccg tagatttcgc
     1861 ctgagtcggc gatgtccttg gcggtgccgt tggtgacgac cagtcgcatc atcgggcgca
     1921 ggctgtcgac catctgcccg gtgatgtcgg tgcgttcgcc ggcgatcgcg gtcgggcggc
     1981 ccaggatcag ttgcggcaca ggcgttttcc ccgcggccac cgtcgcggcc gccagcgcca
     2041 tcccgaacgg gctgaccagc accttgccct gcccgaagcc gtcttcggtg cgctcggcca
     2101 ggttgaccgt cggcggtacc gacccggtca cggtcgggac cccggccacg ttgtagtcga
     2161 ccccgatgcc gtactgggcg gcggcgaccg tcaatgcggt cggcggcatc cggctggcca
     2221 actcggcgaa ggtcgtgttg cacgaactcg cgaacgcccg cgacatcggt accactccga
     2281 ggtcgaagcc tccgtagttc gggatcgtgc ggtggccgat gtccgccgtg ccggggcagc
     2341 ccagcagtgt gttcggggtg gccatgtcgc gctggatggc cgcgccggcg gtgatgatct
     2401 tgaacgtcga ccccggcggg aacaaccccg tcgtcgccag cggaccctcc gcgtctgccg
     2461 aggcgttctg cgcgatcgcc aggatcgcgc cggtcgacgg cctgatcgcg acgatcatcg
     2521 cctgcctgcc gacattggcc accgcgccct gcgcggcgtt ctggacggac cggtccaacg
     2581 tcatcgacac cgaaggggca ggcgtgcccg gcacttcgtt gaggatcccg acgtccaccc
     2641 cgttctggtt gacgctgacc acccgccagc cggcttcgcc gtcgagctgg tcgatcaccg
     2701 actttttgac ctgggcgacg agcgcgggcg cgaacccgtc gtcggtcggc agcatgtcgg
     2761 cctgcggtgt gaccaccacc ccgggcagct ggccgatcgc gggcgcgacg cggttgttgt
     2821 cgtcgggccg cagcgtgatg aggtccaccg gcgtggtcga cgagctggcc tgctcggcga
     2881 gccgctgggg gtcgagcgtg tcgtcgaatg gccgcaacgc ctccaccacg gcacgcgcgg
     2941 tcggcatcag ctgcgtgccg gcctgtgtcg cattgagctc gtagtggtat aggtagccgg
     3001 gtttgagcac gtcggtgccg ccgacctcgt tgaccgacgc gcgttgcggt gggtcggcgc
     3061 gcaacgcgaa cgtctggtgc tcgccgagct tggggtgcag gcccgcgggc gtccaccgga
     3121 cctcccagcg gccctcctcg cgaatcatcg acattcggcc gtcgtaggtc caggtccggt
     3181 tcttcggcag atgccaggtg tagcggtagt cgacggtgcc ggtgtcctcg tcgaagctcg
     3241 aactcaggac ctgggcgtcg aggcgggtgg cctgcaggcc ggcccacgcc gcgttgagtg
     3301 cggcccgcgc gtcgtcgggg cggtcggcca gcgcggcggc cgcgctggtg ttgccggtgg
     3361 ccagcgccgc gaagaactgc tcggcgacag gtgtgggacc gtcgggtctc ggcgtgcagc
     3421 cggccagtac gccggccacg acgagggcca ccgccaacaa atgtgttact cgtgatgcca
     3481 ttgtgactga tgttacgaag cggtcacggt ccaacggcgg aggcgcaccg cgagagcgac
     3541 gttttgcagg ccgtcactcg cagttcttct gcaaaacgtg actctggaga cagagatcag
     3601 tccatcagca cggtcgcgaa cgtgccgacc tccgcgaagc ccacccgcgt gtaggccgcg
     3661 cgggcgatgg tgttgaagtc gttgacgtat aagctggcca tccgtccgct gtcgatgatg
     3721 gacgcgacca ccgccgcggt ccctgcggtg ccgaggccat ggccgcggta ctcgggatga
     3781 acccacacgc cctggatctg gccgacggcc ggcgattgag aacccacctc ggccttgaag
     3841 atcaccttgc cgtgctcaaa ccgcgcccac gcgcgccccg ccgctatcag ccccgcgacc
     3901 cgccggcggt agccacggcc gccgtcacca agccgcgggt cgatgcccac ctctccgatg
     3961 aacatgtcga tcgccgccac gaggtacggc tcgagttcgt cgacgcggac ccgccgcaca
     4021 ccggggtcga tcggagacga cggtcgcgat gccatcgcca tcagcggctg ctgctcgcgc
     4081 acgtcacgcg caggccccca ggtgtgctcg agccgccgcc acatcggcag caccaactcg
     4141 gcacgaccga cgaccgacga gcaccgccgc gcgactccca tcgccttgtc ggcaaatgcg
     4201 tgcagatcgg ctgctgcgcc gcgcagcggt atcaggtttg cgcctgcgta acaaagggat
     4261 tcgtcagcgc cgcggcgcgt ccacagctcg ccgccgatcg cggaaggctc gataccgtgc
     4321 tcggcgaccc gggcggcgac catgcacgac ccgatcggat cggcgtcgat aacccgctgc
     4381 accgcagtcg ggtcgcgcgc caccgacaca cgccgttcgt cgaccaggcg aaagactgga
     4441 ggagccgaca tggacgctct ttctgccgga ttaccccatc agcttacggt cacgacaggc
     4501 gaaccgctgg tatctgaccc gcgctcttca gcgatccgca ttgcctcttc gatcagcgtc
     4561 tcgacgatct gcgactcggg cacggtcttg atcacctcgc ctttgacaaa gatctggccc
     4621 ttgccgttgc ccgacgccac gccgaggtcg gcctcgcgcg cttcgccggg accgttcacg
     4681 acgcagccca tcacggcgac gcgcagtggg acgtccagcc cctcgagccc ggcggtcacc
     4741 tcgttggcca gggtgtagac gtcgacctgc gcacgaccgc acgacgggca cgacacgatc
     4801 tccagcgagc gcggccgcaa gttcagcgat tcgaggatct gattgccgac cttgacctct
     4861 tcgaccggcg gggcggacag cgacacccgg atcgtgtcac cgatgccgcg ggacagtagc
     4921 gcgccgaacg cgaccgcgga cttgatcgtg ccctggaacg cggggcccgc ctcggtgacg
     4981 ccgaggtgca gcgggtactc gcactgcgag gcgagtagtt cgtaggcggc gaccatgacc
     5041 accgggtcgt tgtgcttgac gctgatcttg atgtcgccga agccgtgctc ctcgaacagc
     5101 gacgcctccc agagcgcgga ctcgaccaac gcctcggggg tggccttgcc gtatttctcc
     5161 atgaaccgct tgtccagcga cccggcgttg acgccgatcc ggatcgggat gccggccgct
     5221 ccggcggcct tcgtgacctc agccacccgg ccgtcgaatt ccttgatgtt gccggggttt
     5281 acgcgcaccg ccgcgcagcc ggcgtcgatc gcggcgaaga tgtacttcgg ctggaagtgg
     5341 atgtcggcaa tcaccgggat ctggctgtgc cgggcgatct cggccagcgc gtcggcgtcc
     5401 tcttgccgag ggcaggccac ccggacgatg tcgcagccgg tcgcggtcag ctcggcgatc
     5461 tgctgcagtg tggcgttgac gtcgtgggtt ttggtcgtgg tcatcgactg cacggcgatc
     5521 gggtgatcgc tgccgatccc gacgtcgccg accatcagct ggcgggtctt ccgccgtggc
     5581 gccagggtgg gcgccggagg tgtcggcaag cctaggccga cggtcatggg gtctcctact
     5641 ggaacggcct caacgggttg accacgtccg cggtcacggt cagcagcatg tagccgacca
     5701 ccactatcaa gactacgtat gtggcgggca tgagcttgag gtagttgatc ggcgccgccg
     5761 ccgccatgcc gcgcgccgag cggatcatgt tgcggagctt ctcgaacacc gcgactgcga
     5821 tgtgcccgcc gtcgaacggc aacagcggca gcaggttcac cgcgccgagc acaaagttca
     5881 gctgagccag caagaaccag aacaacaccc aaagatcttg gtcaaccgca tcgccaccga
     5941 tgatcgacgc cccgaccacg ctgatcggcg tctccgggtc gcgctctccg ccgccgatcg
     6001 agtgcaccag cgcgccgacc ttggtcggga tcgtcaccag cgccttgccc agcaacacgg
     6061 agaggtcacc ggtgaacgtg aacgtcgcag gcaccgcggt gagagggttg tactgagtcg
     6121 gccccggcag cgcgctgctg gcgccgatcg cgccgaccgt ggatgcagtg ttggggttct
     6181 ctgaggtgaa ccgctgagtc tgtgtgacgt tcacggtgaa ggtgatcttc tggccgtcgc
     6241 gctccacgtc caccggagtg ggtcccgtga gcggccgcac tgcggaaacg acttcgtcga
     6301 agttgtgcac gtccttgttg ccgaccttga cgatcacgtc gccgggccgg ataccggctt
     6361 cagccgcagg cctggccccg gatccgtcgc acttgggctg ctgatccttc gcgagttgcg
     6421 gtgtgacact gcaggtttcg ccgatcaccg cgcgagtgtc cgggttcagg ttgggcaggc
     6481 cccacacgat cgcgatggca tagatcagca ccaggccgac cacgaaattc atgccgggcc
     6541 cggcgaacag cacagccacc cgcttccaga ccttctgtct gtacatcgcg taggggacct
     6601 cgtcgggctt gagctcctcc accgaggtca tcccggcaat gtcgcagaac ccgcccagcg
     6661 ggatcgcctt gacgccgtac tcggttgagc ccagcttgtt gggccgacgc gtcgaccaga
     6721 gcgtgggtcc gaagccgacg aagtagcggc gcaccttcat tccggtcgct cgcgccgccc
     6781 acatgtggcc gcattcgtgc agcgcgacgg acacgatgat gagtagtgcc agcagtatta
     6841 ggccgagcgc gaacttcatc gggcgggtat gacctcctgt gcgacggcgc gggaaaccgc
     6901 actgttggcc cgggctcggg cccagcgctg cgcgtcaagt acgtcttcca cggtagcggg
     6961 ttcagccgcc cactggtcgg cggcgcccag cacgtcggcg atcgttcgca cgattgcggg
     7021 gaaccggatc cgtccggtca ggaacgcggc ggccgcctcc tcgttggccg cgttgtacac
     7081 cgcggtcata cagccaccga ggcgcccggc ctgccgcgcg aggtccactg cagggaagac
     7141 ggcggcgtcc agcggctcga agtcccagct agcggcggtg gcgaagtcgc aggccgcaga
     7201 gacgcgcggc acccggtccg gccagcccag cgccagcgag atcggcagct tcatgtcggg
     7261 cggactggcc tgcgcaatcg tcgacccgtc ggtgaacgtg accatcgagt gcacgatcga
     7321 ctgcggatgc acgacgacct cgatgtcttc gtagggcacc ccgaacagta ggtgcgtctc
     7381 gatgagctcg aggcccttgt tcaccagcga cgccgagttc agcgtgttca tcgggcccat
     7441 gttccaggtc ggatgggcgc cggcctgttc cggcgtgacg ctctcgaggt cggcggccga
     7501 ccagccgcgg aacggtccac ccgatgcggt cagcaccagt ttggcgacct cgttcgcctc
     7561 ccccgcgcgc aggcactgcg cgagcgcgga gtgctcggag tcgacgggca cgatctgacc
     7621 cggcttggcg gccttgagca ccagcgggcc gccggcgacc agcgactcct tgttggccag
     7681 cgccagccgc gcgccgctgt cgagcgcggc cagcgtcggc ttcagcccga gcgcaccgac
     7741 cagtgcattg agcacgacgt cggcctcggt gttgcgcacc agctcggtgg cggcgtcggg
     7801 accgctgtgg attgcgccga cggcgtcggc tgcgctttcg tcggccaccg cgacgttagt
     7861 gacgccggtg gcgtcgcgct ggtccgcgag cagctgcgcg ttggcgccgc cggcggccag
     7921 gccgaccacc tcgaaccggt ccgggttcgc ggcgatgacc tccagcgcct gggtgccgat
     7981 cgagccggtg ctgccgagga tgagcacacg gatgcgattg ctcacccgcc tattgtgccc
     8041 cggccagcgg ggttggcggc cttccggact acttcgccgg gccgactgcg tttgccgcgt
     8101 cgtcggcggg cttgtcggcg agacggactt tgaaagcgtc gcttaggcgg cccttgatcg
     8161 cgtcggccag ccggcctctg gtcgggctag cggccgacgg gattgtggca ccggccgacg
     8221 gattcgtcgt cccgaccggc gtgttcgtga catcaaaggc gcctagcaag gacttccgag
     8281 ttccctgggc gttcgtcgtg atgttgaacg ggtccacctt ccgaagcggg ttcgacgtat
     8341 cgatcgccgg actcggcttc gcggccagct tgaggttcgt cacctgcgcg tcgaccaccc
     8401 gagcggcagt gagccggctg gtgtcgaggt tgccgggcaa gccgaatacg gtgccggcca
     8461 ggtcaactgg cgcagtcagc cgcttctgga ccgtttggac cgtctgtgga agcgcggggg
     8521 catcaagaat tgtgaacgtg ttcgacccac cggggagtgg cggaatcggc ggcagcggcg
     8581 gcagccagaa cgccaattgg tcgttggcga agaagatgaa cgagttgacc gtgtcaaccc
     8641 cgaattgacc aaggttctgg atgaagttgc cctgtccggt cagccactgg gtggtgttga
     8701 agatcgcact gctgacaatg cgttcgccca agttgtagaa gtacatgact tgaggcgcga
     8761 gccagcccac cccgggaatc caaccgaccg cgtactcggc gagctgaaat ccgtaccgca
     8821 cccacggctc caacgcgccg tagatggcct tgacggtgct gtcgatgttc tgcaggttta
     8881 tcaccggagg ctgcacgttc ggcggcgccg gggaacggcg cgcccaaact gggcggtgtc
     8941 acgatccgtg ccacccagtc cgggatcgag aa
//
LOCUS       DASWBF010000018         4124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_67442, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000018 DASWBF010000000
VERSION     DASWBF010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67442"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VF874_00500"
     CDS             <1..304
                     /locus_tag="VF874_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9174620.1"
                     /translation="IDETRRGKRRWEHHTKTGRWVRVDPWDTGFVDLAGSQGLLGQRE
                     GRTAPPWWTGCKRAQRRVPRGHRLRGDRPGRRLRLSDPHTGAGAQRDAGGRSLPPD"
     gene            207..410
                     /locus_tag="VF874_00505"
     CDS             207..410
                     /locus_tag="VF874_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9174621.1"
                     /translation="MAIDPAAAYASAIRTPGLVPNATLVVDHFHLIDRTNSGTDVCRA
                     RRNRVEGLASCSTADKEALHGRV"
     gene            397..1653
                     /locus_tag="VF874_00510"
     CDS             397..1653
                     /locus_tag="VF874_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9174622.1"
                     /translation="MGEFDSVERDIASIRLPEWGQVVAVDDGVVAFMVVDPDRRPVEP
                     IRRFLRDFVARGRAPGSVRSYAFDLHRWWRFLAAVGVSWDQATSAEVRDFVLWLGQAD
                     KQRRHPRTRSRATAGQINPITRKRHQDDRYQPRTIRHSNAVLRSFYQFAIDEQCGPLV
                     NPVKQERPRGRRANAHHNPLQPFRHEGRLRYNPKLPRQRPRAIPDERWLELFTQMRSN
                     RDRAILALAVSSGARAGELLGMRASDIDWGEQLIQVRRKGSRAAQWLPGSSEAFVWLR
                     LYLDEIGEPESPNDFVWQTLRRHRNRPDAELGRRPMNYEALRAVLRRANNALGTNYSM
                     HDCRHTAAIRMTQSRKLSVPDVQAILGHAHLTTTQIYLVDDDNAVLQRVRQYLAERAA
                     DVEKPVEVATSYDAGDLAVLFGGDQR"
     gene            1650..4049
                     /locus_tag="VF874_00515"
     CDS             1650..4049
                     /locus_tag="VF874_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005195315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9174623.1"
                     /translation="MTAAVALTASNGAVHAELTQGLTRPSRIGPCNDYPIDAVMALLI
                     EHADRLGFSYDRKRRIRRSAPAILEWLQSHPGEGWQQRWLAADADTRLDWLNDLPTIS
                     PVPAREKRAGNVVATNSLLLNRIVLPGYDLLAGWGAMTLYRDTLGSIQPELFSRISDQ
                     AGRNGMVERRKQAGLKVLAQLVLHTGRNVDRLTSDDFDDYRNWGLAHNGFMPPGIVPA
                     WDLLRGIEILPSNITVKAHLHAGQRPTAELVDRYRLRCTAVRDALMRYLDERRPGLDY
                     VSFNALVVHLVGEFWADIERHHPDLDSLNVASEVAEAWKQRVRFVTTKNRPPRPRKSY
                     PQLLMKVRSLYLDIQEWALEDPYWAQHAFPSPVRRTETDGVAKQNRATAAAMHQRVRE
                     RLPKLPVLVETAERGLAAQQALLEAARATPIGGVFVHDDVSYRRTNFKTYQKNPSCSR
                     PDVVLVEHIEAGEQTDAYAAEDDAFWAWAIIETLRHTGIRHEEFLEITHLALVSYRLG
                     DTGDLVPLLQILPSKNNQERLLLVPPELASVLATIITRLRERNGGTVPLVSRYDHHER
                     TFGPMLPHLFQRRTRGHRNEVISTATVQKLLDKALARAHLHDAAGEPLRFTPHDFRRL
                     FATEAVTGGLPVHIAARLLGHEDLSTTQAYLAVFQDDLIRSYRVFLDQRRAVRPEAEY
                     REPTEEEWREFQQYFTLRKVALGTCGRPYGTPCRHEHSCIRCPMLRVDPRQRARLAEI
                     IANLTERIEEAEIHGWLGEKEGLSVSLQAARAKLATLDRPRAQTAHTTTDLGIPITTT
                     H"
BASE COUNT          765 a         1408 c         1336 g          615 t
ORIGIN      
        1 catcgacgag acccgccgcg gtaagcgccg gtgggagcac cacaccaaga ccggccgctg
       61 ggtgcgggtc gacccctggg acaccggatt cgttgacctg gccggctccc agggcctgct
      121 cggtcagcgt gagggccgca cggcgccgcc gtggtggact ggctgcaagc gcgcgcagcg
      181 ccgagttccg cgagggcatc gattacgtgg cgatcgaccc ggccgccgcc tacgcctcag
      241 cgatccgcac accggggctg gtgcccaacg cgacgctggt ggtcgatcac ttccacctga
      301 ttgaccggac taactcgggt actgacgtgt gtcgggcgcg tcggaaccgg gtcgaggggt
      361 tggcttcgtg cagcacagct gacaaggagg cgctgcatgg gcgagtttga cagcgtggag
      421 cgcgatatcg cgtcgatccg gctgccggag tggggtcagg tggtcgcagt ggacgacggc
      481 gtcgtggcgt tcatggtggt cgatcccgac cgacgaccgg tggaaccgat acggcggttc
      541 ctgcgggact tcgtcgcgcg gggccgagcg cccggtagtg tccgcagcta cgcgttcgac
      601 ctgcaccgct ggtggcgatt tcttgccgca gttggggttt cgtgggatca ggcgacgtcg
      661 gctgaggtgc gggacttcgt gttgtggctc ggtcaggccg acaaacaacg ccggcatccg
      721 cgaacgcgat cgcgggcgac ggctgggcag atcaacccga tcacccgcaa acgccatcag
      781 gacgaccgct accagccgcg cacgatccgg cacagcaacg cggtgttgcg tagcttctac
      841 cagttcgcca tcgatgagca gtgcgggccg ctggtcaacc cggtgaagca ggagcggcca
      901 cgcgggcggc gggccaacgc acaccacaac ccgctgcagc cgttccgcca cgagggccgc
      961 ctgcgctaca acccgaaact gccgcggcag cggccgcggg cgatacccga cgagcggtgg
     1021 ctggagctgt tcacgcagat gcgatccaac cgcgaccgtg cgatcctggc gctggcagtg
     1081 agctcgggtg ctcgtgctgg tgagctgctc gggatgcgcg catccgacat cgactggggt
     1141 gaacagctga tccaggtgcg ccgcaagggc tctcgcgccg cgcagtggct gccggggagc
     1201 tcggaggcgt tcgtgtggct gcggctgtat ctcgacgaga tcggcgaacc ggaatcgccg
     1261 aacgatttcg tgtggcagac cctgcgccgc caccgaaacc gcccggacgc ggaattaggc
     1321 aggagaccaa tgaattacga ggccctgcga gcagtgctgc gacgcgccaa caacgcgctg
     1381 ggcacgaact attcgatgca cgattgccgg cataccgcag cgatcagaat gacccagtcg
     1441 cgcaaactgt cggtgccaga cgtgcaggcc atcctcgggc acgcgcacct gaccacgacg
     1501 cagatctacc tggtcgacga cgacaacgcg gtcctgcagc gagtgcgaca atatctcgcc
     1561 gaacgcgccg ccgacgtaga gaagccggtt gaggtcgcaa ccagctacga cgccggcgat
     1621 ctcgccgtgc tgttcggagg tgaccagcgg tgacggccgc cgtggcgctg accgcctcaa
     1681 atggtgcggt gcatgccgaa ctgacgcagg gcctgacacg gccgagccgc atcgggccgt
     1741 gcaacgacta cccgatcgac gcggtcatgg cgttgctgat cgagcacgcc gatcggctcg
     1801 gcttctccta cgaccgcaaa cgccggatcc ggcgcagcgc cccggcgatc ttggaatggc
     1861 tgcagtcgca ccctggagag ggctggcagc agcgctggct ggccgccgac gccgacaccc
     1921 gactggactg gttgaatgat ctgccgacga tctcgccggt cccggcgaga gagaagcgcg
     1981 cgggcaacgt cgtcgcgacc aacagcttgc tgctcaaccg catcgtgctg cccggctacg
     2041 acctgctcgc ggggtgggga gccatgacgc tctatcgaga cacgctagga agcattcaac
     2101 ccgaactctt ttcgcgaatc tccgaccagg ccggccggaa cgggatggtc gagcgccgca
     2161 agcaggccgg tctgaaggtg ctggcccagc tcgtgctgca caccggacgc aacgtggacc
     2221 ggttgaccag cgacgatttc gacgactacc ggaactgggg gctcgcgcat aacgggttca
     2281 tgccgccggg gatcgttccc gcgtgggacc tgttgcgcgg catcgagatt ctgccgagca
     2341 acatcacggt caaggcacac ctgcatgccg ggcagcgccc gaccgcagaa ctcgtcgatc
     2401 ggtaccggct gcgctgcacc gcggttcgcg acgcgctcat gcgctatctc gacgagcggc
     2461 gacctggcct cgactacgtg tcgttcaatg ccttggtcgt ccacctggtc ggggaattct
     2521 gggcggacat cgaacgtcac caccccgacc tcgacagcct caacgtggcg tccgaggtgg
     2581 ccgaggcctg gaagcagcgg gtccgattcg ttaccacgaa gaatcgcccg ccacgcccac
     2641 gaaagtccta cccgcagctg ctcatgaagg tccgcagcct ctatctcgac atccaggaat
     2701 gggcgttgga ggacccgtac tgggcgcagc acgcgttccc cagcccggtt cggcgaaccg
     2761 agaccgacgg tgtcgccaag cagaatcgcg cgaccgcggc cgcgatgcat caacgcgtcc
     2821 gcgagcggct gccgaaatta ccggtgctcg tcgagacggc cgaacgcggc ctcgccgcgc
     2881 agcaagcgct gctcgaagcc gctcgcgcca ctccgatcgg cggcgtgttc gtccacgacg
     2941 atgtcagcta tcggcgcacg aacttcaaga cctaccagaa gaacccgtca tgctcgcggc
     3001 cggacgtcgt gctcgtcgag cacatcgagg ccggcgaaca gaccgacgct tacgccgccg
     3061 aggacgacgc gttctgggcg tgggcaatca tcgagacgct gcgccacacc ggcatccgcc
     3121 acgaagagtt cctcgagatc acccacctgg cgttggtgtc ctaccggctg ggcgacaccg
     3181 gtgacctcgt tccattgctg cagatcctgc catcgaagaa caaccaggaa cggttgctgc
     3241 tggtgccgcc ggagctggcc agcgtgctcg cgacgatcat cacccggttg cgcgaacgaa
     3301 acggcgggac ggtgccgctg gtcagccgtt acgaccacca cgaacgcacc ttcggaccga
     3361 tgctgccgca cctgtttcag cgtcgtaccc ggggtcaccg caacgaggtc atctcgaccg
     3421 ccaccgtcca gaaactgctc gacaaagccc tcgcccgcgc ccacctgcac gacgccgccg
     3481 gtgaaccact gcggttcact ccacacgact tccgccggct cttcgccacc gaggccgtga
     3541 ccggcgggct gcccgtgcac atcgccgccc gactactcgg acacgaggac ttgtccacca
     3601 cacaggccta cctcgcggtg ttccaggacg atctgatccg ctcctatcgg gtattcctcg
     3661 accagcgacg cgccgtgcga ccagaggccg aataccgcga gccgaccgag gaggaatggc
     3721 gggagttcca gcagtacttc acgctgcgca aggtcgcact cggcacctgc ggacgcccct
     3781 acggcacgcc gtgccggcac gagcacagct gtatccgctg cccgatgctg cgggttgatc
     3841 cgcgccaacg cgcccgcctg gccgaaatca tcgccaactt gaccgagcgg atcgaggagg
     3901 ccgaaataca cggatggctc ggcgagaagg aaggcctcag cgtcagcctc caggcggccc
     3961 gcgccaagct cgccacactc gaccgtcccc gagcccaaac cgcacatacg acaaccgatc
     4021 tcggcatccc catcacgaca acacactgaa acaaacgtcg gcgcgaattg cgacgaggca
     4081 ggcacccaac aagatcgatc tagtccggaa aaacctggtc aaac
//
LOCUS       DASWBF010000019        10849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_396721,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000019 DASWBF010000000
VERSION     DASWBF010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396721"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VF874_00520"
     CDS             <1..593
                     /locus_tag="VF874_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HEX9174624.1"
                     /translation="PTESRKTTAPKGRPTPKRDAARRRGPVAPAPMTAAQARRRRKEV
                     GCKLSREERREFRAQQRQRTTERRDKMMAGEEGYLLPRDQGPVRRYVRDVVDSRRNVL
                     GLFMPIAFGFVFVMLAVPVPRVQLLISPAMMALVVVMIGSAFFLGRRVNKLVDQKFPK
                     NTETGWRLGFYAASRATQLRRMRAPRPQVNRGDPVT"
     gene            599..1093
                     /locus_tag="VF874_00525"
     CDS             599..1093
                     /locus_tag="VF874_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX9174625.1"
                     /translation="MRTLVLGGIRSGKSRWAEAAIADAVGPGEPVRYVATGPKEDSDP
                     SWSARIAAHRARRPAHWSTIETDDVATLLRENTTTATLIDDVGGWLASDLDRPDADEL
                     LSAISGFDAALVLVSPEVGLTVVPATASGRRFADELGRLNQRLADICDRVVLVVAGQP
                     VSVK"
     gene            1090..2133
                     /gene="cobT"
                     /locus_tag="VF874_00530"
     CDS             1090..2133
                     /gene="cobT"
                     /locus_tag="VF874_00530"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX9174626.1"
                     /translation="MTFAEIVPPDADAAVAASHRQETLTKPPGSLGRLEDLSVWVAAC
                     QGVCPPRQFERARVVVFAGDHGVAAAGVSAYPPEVTAQMVANIDAGGAAVNALAQVAG
                     AGVRVVDVAVDCDEPLTLVIGAHKVRRSSANLAIEDALTPDEARAAVEAGQRIADEEV
                     DAGADLLIAGDMGIGNTTAATTLVAVITGTEPVAVVGRGTGIDDAGWMRKTTAVRNAM
                     YRARNDAHDPMTLLARCGGADLAAMAGFCAQAAARRTPLLLDGVVVTAAALVADQLAP
                     GARQWWQAGHRSTEPAHTLALARLELEPIVDLKMRLGEGTGALVALPILRAAVATLAS
                     MATFGESSVTDKP"
     gene            2130..2852
                     /locus_tag="VF874_00535"
     CDS             2130..2852
                     /locus_tag="VF874_00535"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX9174627.1"
                     /translation="MIRSLASAFAFATVIPVRGGKIGRGTLTALPVAGIMLGALAAAV
                     LWAGDWAFGPHSPLAGMLAVAALLVTTRGLHVDGLADTADALGCYGPPDRALAVMRDG
                     STGPFAVAAVVVVVVIQGLSFAHAGWPAVMAAVATGRVAAVWACRRSVPAAQGSVLGT
                     MVAGTQPAVVAAAWTLAVVAASVAAGPRPWQGPLVVLIALATAALLVAHCVRRFGGIT
                     GDVLGAAIEVATTVAAVGMAIR"
     gene            complement(2853..3254)
                     /locus_tag="VF874_00540"
     CDS             complement(2853..3254)
                     /locus_tag="VF874_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9174628.1"
                     /translation="MDGAARLAVNDGAQATEERLPVDHAQDLRRRSRGSGGIPAGGVR
                     GEGRLRSRSTRRASDRGLDPDGFARLRAGAVPGVSLRHVYVDDADAVFRRALAAGATA
                     LEEPFDTPYGDRRAMVGDRFGNVFQIAHTTD"
     gene            complement(3233..4339)
                     /locus_tag="VF874_00545"
     CDS             complement(3233..4339)
                     /locus_tag="VF874_00545"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX9174629.1"
                     /translation="METGPLEFTAAPNPHPATDEQRTEIMTNPPFGQHHTDHMVSIDY
                     TEGRGWHDAAVLPYGPIELDPSAIVLHYAQEVFEGLKAYRWADGSIVSFRPEANAARL
                     RSSARRLAIPELPEEVFIESLRQLIAVDGAWVPSAVGEESLYLRPFVFATEPGLGVRP
                     AKQYRYLVIASPAGAYFPRGIKPVSVWLSTEYVRAAPGGTGAAKFGGNYAASLLAQAE
                     ASANGCEQVVWLDAVERRFVEEMGGMNLFFVLGSGGSARLVTPELTGTLLPGITRDSL
                     LQLATDAGFAVEERKIDVDEWQKKAAAGEITEVFACGTAAVITPVSSVKHADGEFTIA
                     DGEPGEVTMALRDTLTGIQRGTFSDTHGWMARLG"
     gene            complement(4363..5457)
                     /gene="gcvT"
                     /locus_tag="VF874_00550"
     CDS             complement(4363..5457)
                     /gene="gcvT"
                     /locus_tag="VF874_00550"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX9174630.1"
                     /translation="MSDIQHGPLEDRHRDLGATFAEFGGWLMPVSYAGTVTEHNATRS
                     AVGLFDVSHLGKASVRGPQAAAFVNSALTNDLRRIRPGKAQYTLCCNESGGVVDDLIV
                     YYVSDDELFLVPNAANTAAVVDALKATAPEGVSVANEHRSFAVLAVQGPRSADVLARL
                     GLPTDMDYMGYVDAEFHGVPVRVCRTGYTGEHGYELLPPWERAAVVFDALVDEVGELG
                     GEPAGLGARDTLRTEMGYPLHGHELSLDISPLQARCGWAIGWRKDAFWGREALLAEKA
                     AGPRRLLAGLRATGRGVLRPELTVLSGDAPVGVTTSGTFSPTLKLGIGLALIDTAAGV
                     QTGQRVDVDVRGRKVECEVVDPPFVEAKTR"
     gene            5419..6561
                     /locus_tag="VF874_00555"
     CDS             5419..6561
                     /locus_tag="VF874_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9174631.1"
                     /translation="MPVFEWPVLDVAHRRYPSRVVDFDALEAAGIANARERAGLIEYL
                     DSLGFTAEEMVEAEGRGRLFGLAGDVLAWSGPPTYSLRTAAEALGVAVDDVAHAWALL
                     GLTVAGPDQLALSQADVDGLATWFALKPSMGDDGAFGFLRVLGAAVARLAEAESTVVR
                     AGAPDIQMDHTHDELATARAYRAIAEFIPRIGALIDAVHRHHLNSARTHFEGVLRDTS
                     ANVECGVGFADLSGFTALTQLLTPAELSKLLNEFDATVSNVVHADGGRVVKFIGDEVM
                     WVSAMPELLLKTAVDLVDHPKARESGLQVRAGLAHGRVLAINGDYFGNVVNLAARLAA
                     AAGPGQILAAQAVHDQLPDWPATAQDPLKLKGFDVPVTAYEVHASR"
     gene            6580..8124
                     /locus_tag="VF874_00560"
     CDS             6580..8124
                     /locus_tag="VF874_00560"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX9174632.1"
                     /translation="MSKEPGYQSPSVTVSSSLSKRASGDAVLIVPVVCTDDRVTVVAD
                     DPYLLSDAVAAIEAALAALGATGGEGQLHRVVVPTLPVASVLTVGLGAPRVGAPPACG
                     GEWPADKVRRAAGVAARSLDKAQSVVTTLSALDLGAAVEGLILGAYKFNEFRSAKTAP
                     KEPGLRKITALAPDTKSATKAQAERAGAIAAAVATARDFVNTPPSHLFPAEFASRAEA
                     LAEAAGLEVEVLDEKALARKGYGGVIGVGKGSSRLPRLVRLTYRGAPKRKGAKTVALI
                     GKGVTFDTGGISIKPAANMHHMTSDMGGAAAVIATTVLAAKLELPIDVIATVPMAENM
                     PSDTAQRPGDVLTQYGGITVEVLNTDAEGRLILADAIARACEDNPDYLIETSTLTGAQ
                     TVALGARTPGVMGSDEFRDRVAAISQRVGENAWAMPLPEELKDDLKSTVADLANVSSS
                     RYAGMLVAGTFLREFVADGVQWAHIDIAGPAYNTASPWGYTGKGGTGVPTRTMFAVLE
                     DIAENG"
     gene            complement(8598..9800)
                     /locus_tag="VF874_00565"
     CDS             complement(8598..9800)
                     /locus_tag="VF874_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9174633.1"
                     /translation="MSDVVVLGSGFAGLWAALAAARRIDELGVAGSEVNITVVSSLPY
                     HDIRVRNYEADLTACRIPLRDVLDPVGVGHIAADVSAIDPASRTVATSLGTRAYDRLV
                     IALGSRVVKPDVPGLREFGFDVDTYDGAVRLQRHLVELARGLHTPAAATAIVVGAGLT
                     GIETACELPARLAALFPDIAARVILIDHNRHVGSDMGASARPVIEAALTANEVQTRLG
                     VSVAAIRENGVTLSTGEELAASTVVWCAGMRANPLTADLGVERDRLGRVAVDEHLRVI
                     GVQGVFAAGDVAAAWMDDRHLSVMSCQHGRPMGRFAGYNVISDLLGEPMLSLRIPWYV
                     TVLDLGPAGAVYTEGWDRQVIASGAEAKATKRTINGRRIYPPLNGSRDEILAAAAPTL
                     QSRPTPEH"
     gene            complement(9970..>10849)
                     /locus_tag="VF874_00570"
     CDS             complement(9970..>10849)
                     /locus_tag="VF874_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9174634.1"
                     /translation="VEGRPPSRAMRRAQVGPPREPFSDTLVSITGAGSGIGRATALEF
                     ARLGAEVVVSDVDEANVRETAKDIAAQRRIAHAYKLDVSDADAVEAFADEVCAEHGVP
                     DIMVNNAGVGQAGRFLDTPAEEWDRVLDINLGGVVNGCRAFARRLVERGSGGHIVNVA
                     SAAAFAPLQSLNAYCTSKAAVYMFSDCLRAELDAAGIGLTTVCPGPVHTNIVSTARFD
                     APPGKVPQVSGRRRQWEKFFAARRYGPEKAAKAIVSAVQKNKPIRPVTPEAYLLYGTS
                     RLAPQALRSTARGQVM"
BASE COUNT         1680 a         3742 c         3756 g         1671 t
ORIGIN      
        1 agcccaccga atcccgcaag accaccgctc caaagggccg gccgacgccc aaacgcgacg
       61 ccgccagacg ccgcggaccg gtcgcgcccg cgccgatgac cgccgcgcag gccaggcgcc
      121 ggcgcaagga ggtgggctgc aagctcagcc gcgaggaacg ccgcgaattc cgggctcagc
      181 agcgtcagcg cacgacggag cgtcgcgaca agatgatggc cggcgaggag ggctacttgc
      241 tgccccgcga ccaggggccg gtgcgacgct acgtgcgcga cgtggtggac tcccgccgca
      301 acgtgctcgg cctgttcatg ccgatcgcgt tcggctttgt gtttgtgatg ctggcggtcc
      361 cggtgcccag agtgcaactg ctgatctccc ccgcgatgat ggcgctggtg gtggtcatga
      421 tcggcagtgc gttctttctg ggccgcaggg tgaacaagtt ggtcgaccag aagttcccga
      481 agaacaccga aaccggttgg cggctgggct tttacgctgc gagccgggcc acgcagctac
      541 gccgcatgcg ggcgccgcga ccgcaggtca accgcggcga ccccgtcacc tgaccgccgt
      601 gcggacgctg gtgctcggcg gcatccggtc gggcaagtcg cgatgggcgg aagcggcgat
      661 cgccgacgcc gtcggccccg gcgaaccagt gcggtacgtc gccaccggac cgaaagaaga
      721 ctccgacccg tcgtggtcgg cacgcatcgc cgcgcaccgg gcacgtcggc cggcgcactg
      781 gtcgacgatc gaaaccgacg acgtcgcgac actgttgcgt gagaatacga ccaccgcgac
      841 gttgatcgac gatgtcggcg gttggctcgc ctccgacctg gacagaccag atgctgacga
      901 attgctctca gcgataagcg gtttcgacgc agctctggtg ttggtcagtc cagaagtagg
      961 gctgacggtg gtgccggcca ccgcgtccgg ccggcggttc gccgacgaac tcggccggct
     1021 caaccagcgg ttggccgaca tctgcgatcg cgtggtgctg gtggtcgctg gtcagccggt
     1081 gagcgtgaaa tgaccttcgc cgagatcgtt cctcccgacg ccgacgcggc cgtcgccgcg
     1141 agccatcgcc aggaaacgtt gaccaaacca ccgggctccc tgggtcggct cgaagatctg
     1201 tcggtgtggg tggccgcgtg tcaaggagtc tgtccgccaa ggcaattcga gcgcgcccgg
     1261 gtggtggtgt tcgccggcga ccatggcgtc gcggcggccg gggtgtcggc gtacccgccg
     1321 gaggtcacgg cccagatggt ggcgaacatc gacgccggtg gcgcagcggt caacgcactg
     1381 gcccaggtcg ccggggccgg ggtgcgggtg gtcgacgttg ccgtcgactg cgacgagccg
     1441 ctgacccttg tgatcggcgc ccacaaggtc cgccggagca gcgcaaacct ggccatcgag
     1501 gacgcgttga ccccggacga ggcgcgcgcg gccgtcgagg cgggccagcg gatcgccgac
     1561 gaagaggtcg acgccggcgc cgacttgctg atcgccggcg acatgggaat cggaaacacc
     1621 acggcggcaa ccactttagt ggcggtcatc accggcaccg agccggtcgc ggtggtcggg
     1681 cgcggcaccg ggatcgacga cgccgggtgg atgcgcaaga cgaccgccgt gcgcaacgcg
     1741 atgtaccggg cgcgcaacga cgcgcatgac ccgatgacgt tgctcgcgcg ctgcggcggc
     1801 gcggacctcg cggcgatggc cgggttctgc gcacaagccg ccgcgcggcg caccccgctg
     1861 ctgctcgatg gcgtcgtcgt gacggccgcg gcgctggtcg ccgaccagct ggccccgggc
     1921 gcgcggcagt ggtggcaggc cggccaccgg tccaccgaac ccgcgcacac cctcgcgctg
     1981 gcgcgcctgg aactcgagcc aatcgtcgac ctcaagatgc ggctcgggga gggcaccggt
     2041 gcgctggtgg ccctgccgat cctgcgggcg gcagtggcca cgttggcgtc gatggccacg
     2101 tttggcgagt cctcggtgac cgacaagccg tgatccgttc tcttgcaagc gctttcgcgt
     2161 tcgccacggt gatccctgtc cgcggaggga agatcgggcg gggcacgctg accgcgctgc
     2221 ctgtcgccgg aatcatgttg ggtgccctgg ccgccgcggt cctctgggcg ggcgactggg
     2281 cgttcgggcc gcacagtccg ctggccggga tgctggcggt cgcggcgctg ctggtgacga
     2341 cccgcggcct gcacgtcgac ggcctggccg acaccgctga cgcgcttggc tgctacggcc
     2401 cgcccgaccg cgcgctggcc gtgatgcgcg acggctcgac cgggccgttc gcggtggctg
     2461 cggtcgtggt ggtggtggtt atacaggggc tgtcgttcgc gcacgcgggc tggccggctg
     2521 tgatggccgc ggtggcgacg ggccgggtcg ccgcggtgtg ggcgtgccgg cgcagcgtgc
     2581 ccgccgcgca gggcagcgtg ctgggcacca tggtcgcggg cacgcaacca gcggtggtgg
     2641 cggcggcgtg gacgctcgca gtcgtggcgg cgtcggtggc ggccggtccg cggccgtggc
     2701 agggaccgct ggtggtcctg atagcgctgg cgacggcggc cctgctggtc gcgcactgcg
     2761 tgcgccggtt cggcgggatc accggcgacg tgctcggcgc ggcgatcgaa gtggcgacga
     2821 cggtcgcggc cgtggggatg gcgatccgct agtcaatccg tcgtgtgtgc gatctggaag
     2881 acgttgccga accggtcccc gaccatcgct cggcggtctc cgtacggggt gtcgaacggt
     2941 tcctcgagcg ctgtggcgcc tgccgcgagc gcgcggcgga acacggcgtc ggcatcgtcc
     3001 acatagacgt gacgtagaga aacgccggga acggctcccg ctcggaggcg ggcgaaacca
     3061 tcaggatcga gtccccgatc tgaagctcgg cgggtcgacc tggaacgtag tctcccttcg
     3121 cctcgaacac ctcctgcagg aatgccacca gagccgcggg atcgtcgacg aagatcctgg
     3181 gcgtgatcga ccggtaacct ctcctcggtc gcctgtgcac catcgttgac ggctagccga
     3241 gccgcgccat ccagccgtgc gtgtccgaga aggtgccgcg ctggatgccg gtcaacgtgt
     3301 cgcgcagcgc catcgtcacc tcgccgggtt cgccgtcggc gatcgtgaac tcgccgtcgg
     3361 cgtgcttcac cgacgacacc ggcgtgatca ccgcggcggt gccgcaggcg aagacctcgg
     3421 tgatctcccc cgccgccgcc ttcttctgcc actcgtcgac gtcgatcttg cgttcctcca
     3481 ccgcgaagcc ggcgtcggtg gccagctgca gcagcgagtc gcgcgtgatg ccgggcagca
     3541 gcgtgccggt gagctcaggg gtgaccagac gcgccgaccc gccgctgccg agaacgaaga
     3601 acaggttcat gccgcccatc tcctcgacga accggcgctc gaccgcgtcg agccagacca
     3661 cctgttcgca gccgttcgcg ctggcttcgg cctgcgccag cagcgacgcg gcgtaattgc
     3721 caccgaactt ggcggcgccg gtgccgccgg gagccgcgcg cacgtactcc gtcgacagcc
     3781 acacgctgac cggcttgatg ccgcgcggga agtaggcacc ggccggtgac gcgatcacca
     3841 ggtagcggta ctgcttggcg ggccgcacgc ccagtcccgg ctcggtggcg aacacgaacg
     3901 gccgcagata cagggactcc tccccgaccg cggacggaac ccaggcgccg tccaccgcga
     3961 tcagctgccg cagcgactcg atgaacacct cttcgggcag ttcggggatg gccaaccgcc
     4021 gcgccgacga ccgcagccgt gccgcgttgg cctccgggcg gaacgacacg atcgacccgt
     4081 cggcccaccg gtacgccttg aggccctcga acacttcttg cgcgtagtgc agcacgatcg
     4141 ccgacgggtc cagttcgatc ggcccgtacg gcagcacagc cgcgtcgtgc cagccccgcc
     4201 cttcggtgta gtcgatcgac accatgtggt cggtgtgatg ctggccgaac ggcggattcg
     4261 tcatgatttc ggtccgctgc tcgtcggtcg ccggatgcgg gttgggtgca gcggtgaact
     4321 cgagagggcc ggtttccatg cgagcgattg tacgtccggg ggctagcgag ttttggcctc
     4381 cacgaacggc ggatcgacga cctcgcactc caccttgcgg ccgcggacgt cgacgtccac
     4441 gcgctgcccc gtctgcacgc ccgcggcagt gtcgatcagc gccagcccga tgccgagttt
     4501 cagtgtggga gagaaggttc ccgacgtggt gacgcccacc ggggcgtcgc cggacagcac
     4561 cgtcagctcg gggcgcagca cgccgcggcc cgtcgcgcgc agcccggcca acagccggcg
     4621 cgggcctgcc gccttctccg ctagcaacgc ttcgcgcccc cagaacgcgt ccttgcgcca
     4681 cccgatcgcc catccacagc gggcctgcag cggtgagatg tcaagcgaca gctcgtgacc
     4741 gtgcagcgga tagcccatct cggtgcgcag cgtgtcgcgg gctccgagac cggccggctc
     4801 gccgccgagc tcgccgacct cgtcgaccag cgcgtcgaac accaccgccg cccgctccca
     4861 cggcggcagc agctcataac cgtgctcgcc ggtgtagccg gtgcggcaca cccgtaccgg
     4921 aaccccgtga aattccgcgt cgacgtagcc catgtagtcc atgtccgtcg gcaggcccag
     4981 ccgcgccagc acgtcggccg agcgcgggcc ctgaacggcg agcaccgcga atgaccgatg
     5041 ctcgttggcc accgaaacac cttcaggcgc tgtggctttc aatgcgtcga ccacggccgc
     5101 ggtgttcgcg gcgttgggca cgaggaacag ctcgtcgtcg gagacgtagt agacgatcag
     5161 gtcgtcgacg acgccgccgg attcgttgca gcacagcgtg tattgcgcct tgcccgggcg
     5221 gatgcggcga aggtcgttgg tcagcgcgga gttgacgaac gcggccgcct gcggtcctcg
     5281 caccgacgcc ttgccgaggt ggctgacgtc gaacaggccg acggccgagc gggtggcgtt
     5341 gtgctcggtg acggtgccgg cgtatgagac cggcatcagc cagccgccga actcggcgaa
     5401 cgtggcaccg aggtcacgat gccggtcttc gagtggcccg tgctggatgt cgctcaccgc
     5461 cgatacccta gtcgggtggt cgatttcgac gcgttggagg ccgccggaat cgcgaacgca
     5521 cgcgaacggg ccggcctcat cgagtacctc gacagcctcg gattcaccgc cgaggaaatg
     5581 gtcgaggccg agggccgcgg ccggctgttc ggcctggcgg gtgacgttct ggcgtggtcg
     5641 ggccctccca cctacagcct gcgcaccgcc gccgaggcgc tcggcgtagc ggtcgacgac
     5701 gtcgcgcatg cctgggcact gctcggcctg accgtcgccg gcccggacca gctcgcgctc
     5761 agtcaggctg acgtcgacgg tctggccacc tggtttgcgc tcaagccgtc gatgggcgac
     5821 gacggggcgt tcggctttct gcgtgtcctg ggcgcggccg tggcccgact cgcggaggcc
     5881 gagtcgacgg tcgtccgcgc tggggcaccc gacatccaga tggaccacac ccatgacgaa
     5941 ctcgccaccg cgcgggcgta tcgcgcgatc gcagagttca ttccgcgcat cggcgcattg
     6001 atcgacgccg ttcaccgcca tcacctcaac agcgcccgaa cccatttcga gggcgtcctg
     6061 cgagacacgt cggcgaacgt ggagtgtggc gtcggcttcg ccgatctgtc gggattcacc
     6121 gcgctgacac agctcctcac ccccgccgag ttgtcgaagc tgctcaacga gttcgacgcc
     6181 accgtcagca acgtcgtgca tgccgacggt gggcgggtgg tgaagttcat cggcgacgag
     6241 gtcatgtggg tcagtgccat gcccgagctg ctgttgaaga cggcggtcga cctcgtcgat
     6301 caccccaaag cacgggaatc cgggctgcag gtccgcgccg gtctggccca cggcagggtg
     6361 ctggccatca acggcgacta cttcggcaac gtggtcaacc tcgccgcccg gctggccgcc
     6421 gcggcggggc ccggacagat tcttgcggcc caggccgtgc acgaccagtt accggattgg
     6481 ccggctacgg cgcaggatcc gttgaaactc aagggtttcg acgtcccggt cacggcctac
     6541 gaggtgcatg ccagccgctg agcgctaggg tggttgcccg tgagcaaaga acctggctat
     6601 caatcaccct cggtcaccgt cagctcgtcg ctgtcaaagc gcgcgtcggg tgacgcggtg
     6661 ttgatcgtgc cggtggtgtg caccgacgac cgggtgacgg ttgtggccga cgacccctac
     6721 ctgctcagcg acgccgtcgc cgcgatcgaa gccgcgctcg ccgcactcgg ggcgaccggc
     6781 ggcgaaggac agctgcaccg cgtcgtggtc ccgacgcttc cagtggcgag cgtgttgacc
     6841 gtcggactcg gagcaccgcg cgtgggggca ccacccgctt gcgggggaga gtggccggct
     6901 gacaaagtcc gccgtgctgc cggagtggcc gctcggtcgc tggacaaggc gcagtcggtg
     6961 gtgaccacgc tgtcggcgct cgacctcggc gccgcggtgg aggggctgat cctcggcgcc
     7021 tacaaattca acgagttccg cagcgccaag accgcgccga aggagccggg cctgcgcaag
     7081 atcaccgcgc tcgcgccgga caccaagtca gccaccaagg cccaggcgga gcgcgccggc
     7141 gcgatcgcgg ccgcggtggc caccgcccgc gatttcgtca ataccccacc gagccacctc
     7201 tttccggccg aattcgcttc ccgcgcagag gcattagccg aagctgccgg cttggaggtc
     7261 gaagtgctcg acgagaaggc gctggcccgc aagggctacg gcggggtgat cggcgtcggc
     7321 aagggttcgt cgcgcctgcc gcggctggtc cgcctgacct atcgtggcgc cccgaaacgc
     7381 aagggcgcca agaccgtcgc actgatcggt aagggcgtca ccttcgacac cggcggcatc
     7441 tcgatcaagc cggcagccaa catgcaccac atgacctccg acatgggcgg ggccgcggcc
     7501 gtaatcgcga cgacggtgct tgccgcgaaa ctggagctgc cgatcgacgt gatcgctacg
     7561 gtgccgatgg ccgagaacat gccgtccgac accgcccagc ggcccggcga cgtgctcacc
     7621 caatacggcg gcattacggt cgaggtgctc aacaccgacg ccgagggacg gttgatcctc
     7681 gccgatgcca ttgcccgcgc gtgcgaggac aacccggact atctgatcga gacgtcgacg
     7741 ctcaccggcg cgcagaccgt cgcgctcggc gcccgcacgc ccggcgtgat gggcagcgac
     7801 gagttccgcg accgggtcgc tgcgatcagc cagagggtgg gcgagaacgc atgggcgatg
     7861 ccgctgccgg aggaactcaa ggacgacctc aagtcgaccg tcgcggatct ggccaatgtc
     7921 agcagctcgc gatacgccgg aatgctggtc gccggaacgt ttctgcgtga gttcgtcgcc
     7981 gacggcgtcc agtgggcaca catcgacatc gccggaccgg cctacaacac cgcaagcccg
     8041 tggggctaca ccggcaaggg cggcaccggt gtgccgacgc gcacgatgtt cgcggtcctc
     8101 gaagacatcg ccgaaaacgg ctgagaagcc cgagccgtga acggcttaca tggccggggc
     8161 cggtcgtcgc gcacccctga catcctgtct gagaacgtct tcgcccctcc gtctgcgggg
     8221 cggtccgagc tgtcgttctg ctgttgtctt actagtcgcg cggcactccc actttcgcga
     8281 cgctgcgact ttgcgatttg gcgccgttcg gaccgcgcag gcaggtgtgg caaatatggt
     8341 ctgtttcgag acacccacgc cattagccac gtgagcgaga acagcccagc ggccaacaaa
     8401 gatgtagcac agcgctagcc gccgcacatc ccagcactgg cgggcgtgcg ctcgcgtcgg
     8461 gcggaaccca cgagggaccc tgacagccac cgcagacagt gcagcaccgt aaccgatgac
     8521 cttccacgac aacatcgaaa cggaacaacg cgcgttcggg tttgttgggg gcaaccgtag
     8581 gcttcgatgg tgccaggtca gtgctcgggg gttggccgtg attgcagcgt cggtgcggcg
     8641 gcggcgagga tctcgtctcg actcccgttg agagggggat agattcgcct gccgttgatt
     8701 gtgcgcttgg tggccttggc ttcggccccg ctggcgatca cctgccgatc ccagccttcg
     8761 gtatagactg cccccgccgg cccgaggtcc aaaactgtga cataccaggg aattcgaagg
     8821 gacaacatcg gctcgccaag tagatcgctg atgacgttgt acccggcgaa ccgccccatc
     8881 ggcctgccgt gctggcagga catcaccgat aggtgtcgat cgtccatcca cgcggccgca
     8941 acatccccgg cggcgaacac cccctgcacg ccgatcacgc gcaggtgttc gtcaaccgca
     9001 acccggccga gacggtcacg ctcgacgccc agatccgcgg taagcggatt ggcgcgcata
     9061 ccggcacacc acaccacggt cgacgcggcc agctcttccc ctgtcgacaa tgtcacccca
     9121 ttttcacgga tcgctgcgac actgaccccg agccgggtct gcacctcgtt cgccgtcagc
     9181 gcggcctcga tcactgggcg cgccgatgcg cccatgtccg agccaacgtg ccggttgtga
     9241 tcgatgagta tgacgcgggc agcgatgtcc gggaatagcg ccgccagccg cgcaggcagc
     9301 tcgcaggcgg tctcaatgcc ggtcagccca gcgcccacca cgatcgcggt cgccgcggcc
     9361 ggtgtgtgca gaccgcgcgc gagctctacc aggtgccgct gcagccttac cgcgccgtcg
     9421 taggtgtcaa cgtcgaagcc gaactcacgc agaccgggga cgtccggttt gaccactcgg
     9481 ctgcccaacg cgatcacgag acggtcgtag gcccgcgtac cgagcgaggt ggccaccgtg
     9541 cgagaagccg ggtcgattgc cgacacatcg gcggcgatgt gccccactcc gaccgggtcg
     9601 agcacatccc gcagcggaat tcggcacgcc gtcaggtcgg cctcgtagtt gcgcacccgg
     9661 atgtcgtggt aaggcagtga gctgactacc gtgatgttca cctcgctgcc cgcgacgccg
     9721 agttcgtcga ttcgccgggc cgcagccagg gcggcccaca gaccggcgaa cccggaaccg
     9781 agcacaacca cgtcgctcac cgggccaacg ttagccgcca ggtacccgcg atgcggcgac
     9841 ccgacgcccc aatcgagccg aattgcctca tcgccctggg gcacggtcac cggataaacg
     9901 tggtcttgag accaacttct gcgagttgca gttgtggtct ctttcgagtc agtgggcaga
     9961 tctgcagtgt cacatcacct ggccgcgcgc ggtgctgcgc aacgcctgcg gcgccaggcg
    10021 cgacgtgccg tacagcaggt aggcctccgg cgtgaccggg cggatcggct tgttcttctg
    10081 caccgccgac acgatggcct tcgccgcctt ctcgggaccg taacgccgtg cggcgaagaa
    10141 cttttcccat tgcctgcgcc gccccgacac ctgtggcacc ttgcccggcg gcgcgtcgaa
    10201 ccgggcggtg ctcacgatgt tggtgtggac ggggccgggg cacacggtgg tcaaaccgat
    10261 tccggcagcg tcgagttcgg cccgcaggca gtcggagaac atgtacaccg ccgccttgct
    10321 ggtgcagtac gcgttcagcg attgcagcgg cgcgaacgcc gccgcggatg cgacgttgac
    10381 gatgtggccg ccgctgccgc gctcgacgag ccggcgcgcg aacgcccggc agccgttgac
    10441 gactccgccg aggttgatgt cgaggacccg gtcccattcc tcggccgggg tgtccaggaa
    10501 ccgtcccgcc tggccgacgc cggcgttgtt caccatgatg tcggggacgc cgtgctcggc
    10561 gcacacctcg tcggcgaacg cctcgaccgc atcggcgtcg gacacgtcca gcttgtacgc
    10621 gtgtgcgatt ctacgctgtg ccgcaatgtc tttcgcggtt tcccgtacgt tggcctcgtc
    10681 gacgtcgctg accaccactt cggcgcccag ccgggcgaat tcgagggcgg tggcgcggcc
    10741 gatgccgctt cctgcgccgg tgatcgacac cagtgtgtcg ctgaacggct cccggggcgg
    10801 gccgacctgc gcacgccgca tcgcccggga cggcggacgt ccttcgacg
//
LOCUS       DASWBF010000020         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_396743,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000020 DASWBF010000000
VERSION     DASWBF010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396743"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="VF874_00575"
     CDS             <1..153
                     /locus_tag="VF874_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HEX9174635.1"
                     /translation="YRRAGVGVAVTAADRAHVIEVLDAAERLVAARPEMALLSARRGV
                     HRSDDF"
     gene            complement(155..979)
                     /locus_tag="VF874_00580"
     CDS             complement(155..979)
                     /locus_tag="VF874_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX9174636.1"
                     /translation="MRRTGPFEVGDRVQLTDAKGRHYTMILTPGAEFHTHRGYVSHDA
                     VIGQPEGSVVKATNGDQFLVLRPLLVDYVMSMPRGAQVIYPKDAAQIVHEGDIFPGAR
                     VLEAGAGSGAMTCSLLRAVGPAGRVISYEARDDHAAHAERNVVTFLGERPTNWDLVIA
                     DVADYAGEQVDRAVLDMLAPWEVLDAVAAAVVAGGVLVVYVATVTQLSRTVEALRAQQ
                     CWTEPRSWETLQRYWDVVGLAVRPEHRMRGHTAFLISARRLAPGAVAPTPARRKRR"
     gene            1003..1937
                     /locus_tag="VF874_00585"
                     /pseudo
     CDS             1003..1937
                     /locus_tag="VF874_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558649.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
BASE COUNT          313 a          633 c          659 g          332 t
ORIGIN      
        1 tatcgccgcg ccggagtcgg tgtggcggtg acggcggccg accgcgcgca cgtgatcgag
       61 gtgctcgatg cggccgagcg gctggtggcg gcacggccgg agatggcatt gctgtcggct
      121 cggcgcgggg tgcaccgcag cgacgacttc tgaactatct gcgtttgcgc ctcgccggcg
      181 tcggggcgac ggcgccggga gcgagccgcc gtgcgctgat cagaaacgcg gtgtggccgc
      241 gcatccggtg ctcgggtcgc acggccagcc cgaccacgtc ccagtaacgc tgcagcgtct
      301 cccacgaccg tggttcggtc cagcactgct gtgcgcgcag cgcctcgacc gtgcgcgaca
      361 gctgggtgac cgtcgcgacg tacacaacca gcaccccacc ggccaccacc gccgcggcca
      421 ccgcgtcgag cacctcccac ggggccagca tgtcgagcac cgcccggtcg acctgctccc
      481 cggcgtaatc cgcgacgtcg gcgatgacga gatcccagtt ggtcggccgc tcacctaaga
      541 acgtgacgac gttgcgttcg gcgtgtgcgg cgtggtcgtc gcgcgcctcg taagagatca
      601 cccggcccgc cgggccgacg gcccgcagca gcgagcacgt catcgcgccg gacccggcgc
      661 cggcctcgag cacccgagcg cccgggaaga tgtcgccctc gtgcacgatc tgggcggcgt
      721 ctttggggta gatcacctgc gcgccgcgcg gcatcgacat cacgtagtcc accaacagcg
      781 gccgcagcac caggaactgg tcgccgttgg tggccttgac cacgctgcct tcgggctggc
      841 cgatcaccgc gtcgtgggac acgtagccgc ggtgcgtgtg aaactccgct cccggcgtca
      901 agatcatcgt gtagtgacgg cccttggcgt cggtgagctg cacgcggtcg cccacctcaa
      961 aaggacccgt acgacgcaca ggttcaccct attcttggcg gaatggcgga ctcatgtccg
     1021 tcctgtggca ccgacctgct ggcgaacgcg aagttctgcc actcgtgtgg ctcgccgatt
     1081 caagcatctg gtgaccgagc cgaatacaag caggtgacgg tgctcttcac cgacgtggtc
     1141 cgatcgatgg ccatcgcctc agccgtcgac gtcgaacgac ttcgcgagat catggccgag
     1201 ctcttcacgc ggtcggcggc ggtcgtgcag cgctacggcg gcacggtaga caagttcacc
     1261 ggcgacggca tcatggcgtt gttcggagca ccaattgcat tggaggatca tgccgttcgg
     1321 gcatgccggg ccgcgctgga aattcaggat gcggctcgag agttggcccg tgaagtcgaa
     1381 cgcaccgacg gcgtcgatct ggagatacga gtcgggctta attccggccg tgtgatcgcc
     1441 ggcgaaatag cctccggtgc agcgggttac accgccgttg gggaacaagt cgacctggcc
     1501 caacggatgg agtcggtggc cccggcgggt ggcgtaatgc tcagcgcgtc gaccgcgcgt
     1561 ctcgttgaac atgcagccgt cttgggagat tccgaactcg tgcaatcaaa ggcgcggcct
     1621 ctgcggtgcc ggcccgccga ctcatcggca tggcggcaca gccaatgcat gcgccccgct
     1681 gggaaacgac gctggttggc cggacgtggg aattaggctc gctgacaggg attttggatc
     1741 aatctgttta cggccagggt ggcatcgtca gcgtcattgg tcctccaggc atcggcaaga
     1801 gtcgaatggt tcgagagaca acggcgatcg ctcgcgctcg cggggtcaag gtgtttagcg
     1861 ccttctgcga atcgctcacc acgaccgttg cgttcggcgt tgcctcagct cttgcggtcg
     1921 gttgtcggag tcacaga
//
LOCUS       DASWBF010000021         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_132679,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000021 DASWBF010000000
VERSION     DASWBF010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132679"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF874_00590"
     CDS             complement(<1..630)
                     /locus_tag="VF874_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174637.1"
                     /translation="MAAELDVAGRLAEARQAVDNTQSYVWACHLLDYQNPDLTLHSAQ
                     VRDWYSSEDGMDLRVLDADCSALQAVAAAADDALRRQREQVTSLAAAWRGDGAASAQD
                     FLRRHADTAENATTAVRTAADAVAGLRDALWAIVDKKVATLSAIDDRRQGERPAWLAA
                     AQTVISGAGDRAAASELLDQQVKPFVDNDIRVDWLTAVRTATASIGSSYD"
     gene            complement(634..936)
                     /locus_tag="VF874_00595"
     CDS             complement(634..936)
                     /locus_tag="VF874_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HEX9174638.1"
                     /translation="MGPQTARVDVAAVRDVAAEFDASAAALDAAVGTHLSHLGFGGAT
                     AGRAYATRGDALRTALDRLADGLTAWSRASVEIAAALRASADRYQRSDESAAARLG"
     gene            complement(974..2308)
                     /gene="glmM"
                     /locus_tag="VF874_00600"
     CDS             complement(974..2308)
                     /gene="glmM"
                     /locus_tag="VF874_00600"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9174639.1"
                     /translation="MGRLFGTDGVRGVANRELTAELALAIGSASARRLGATSAGRSVA
                     VIGRDPRASGEMIEAAVIAGITSEGVDALRVGVLPTPAVAYLTSAYDAEFGVMISASH
                     NPMPDNGIKVFGPGGHKLDDATEDDIEQLITTDPAQRPVGAGIGRVIDAEDALERYLR
                     HVGKAVTTRLDGVTVVVDCAHGAASAAAPRAYQAAGARVIAINAAPNGLNINDGCGST
                     HIDALRAAVIAHNADLGLAHDGDADRCLAVDASGDVVDGDAIMVILALAMREAGELAT
                     DTLVATVMSNMGLHLAMRAVGIDVRTTAVGDRYVLEELRAGGYALGGEQSGHIVMPSL
                     GTTGDGIVTGLRLMSRMAQTGRPLRELASAMSTLPQVLINVEVADKSSVAAAPSVQAA
                     VADAEAELGDTGRILLRPSGTEQLVRVMVEAPDEDTAKQVAIRVADCVKFVS"
BASE COUNT          347 a          956 c          938 g          415 t
ORIGIN      
        1 gtcgtaactg ctgccgatcg acgcggtcgc cgtacgtacc gctgtcagcc agtcgacgcg
       61 aatgtcgttg tctacgaagg gttttacttg ctggtcaagc agttcacttg ctgccgcgcg
      121 gtcgccggcg ccggagatga cggtctgcgc ggcagccagc caggcaggcc gctccccttg
      181 tcggcggtcg tcgatcgcac tcagggtcgc gaccttcttg tcgacgatcg cccacagcgc
      241 gtcacgcagg ccggcaaccg cgtccgccgc cgtgcgcaca gccgtggtgg cgttctcggc
      301 cgtgtcggca tgccggcgca ggaaatcctg ggccgacgcc gcgccgtcgc cccgccacgc
      361 cgccgcgagg ctggtgacct gttcacgttg ccggcgcagt gcgtcgtccg cggcggccgc
      421 caccgcttgc agcgcagaac agtcggcgtc cagcacgcgc aggtccatgc cgtcctcgct
      481 ggagtaccag tcgcggacct gcgccgagtg caacgtcagg tcggggttct ggtagtcgag
      541 caggtgacac gcccacacgt aggactgcgt gttgtcgacc gcttggcgcg cttcggccag
      601 ccggcccgcg acgtcgagtt cggcggccaa cggctatccc aaccgcgcgg cggcgctctc
      661 gtcggagcgc tggtagcggt cggcgctggc acgcaacgcg gccgcaatct cgacggacgc
      721 gcgcgaccac gccgtcagcc cgtcggccag ccggtccagc gcggtgcgaa gagcgtcgcc
      781 gcgggtggcg taggcgcgac cggcggtggc acccccgaag ccgaggtgcg acaggtgagt
      841 gccgacggcg gcatcgaggg cggccgccga cgcgtcgaac tcggcggcga cgtcgcgcac
      901 cgcagccacg tcgacccgcg cggtctgtgg tcccataacg gctacgacgc ggcggcgcgt
      961 attccggttc cgctcagctc acgaacttca cacagtccgc gacccgaatc gcgacctgtt
     1021 tggcagtgtc ctcgtcgggc gcctccacca tcacccggac gagctgttca gttcccgagg
     1081 ggcgcaacaa gattcggccc gtgtcgccga gttcggcttc ggcgtcggcg acggccgcct
     1141 gcacggacgg tgcggccgcg accgaactct tgtcggcgac ctcgacgttg atcagcacct
     1201 gcggcagcgt gctcatcgcc gacgccagct cacgcagcgg gcgacccgtc tgcgccatcc
     1261 gcgacatcag ccgaaggccg gtcacgatcc cgtcgccggt ggttcccagc gagggcatca
     1321 cgatgtgccc ggactgctcg ccaccgagcg cgtagccgcc ggcacgcagc tcctcgagca
     1381 cgtagcggtc gcccaccgcg gtggtgcgca cgtcgatgcc cactgctcgc atcgccaggt
     1441 gcaggcccat gttgctcatc accgtcgcga ccagcgtgtc cgttgccagt tcgccggcct
     1501 ctcgcatcgc cagcgccagg atcaccatga tcgcgtcgcc gtcgacgacg tcgccgctcg
     1561 cgtcgacggc caggcaccgg tcggcgtcac cgtcgtgagc caacccgagg tcggcgttgt
     1621 gggcgatcac ggcggcccgc agcgcgtcga tgtgtgtcga cccgcagccg tcgttgatgt
     1681 tcaggccgtt gggtgcggcg ttgatcgcga tgacgcgggc tccggcggcc tggtaggcgc
     1741 gcggtgctgc cgcggacgcg gcgccgtgcg cgcagtcgac gacgacggtg acgccgtcca
     1801 gccgggtggt gacggctttg ccgacatggc gcaggtaccg ctcgagggcg tcctccgcgt
     1861 cgatgacgcg gccgatcccg gcgccgaccg gccgctgggc cgggtcggtc gtgatcagct
     1921 gctcgatgtc gtcctcggtc gcgtcgtcga gcttgtggcc gcccggcccg aagaccttga
     1981 tgccgttgtc gggcatcggg ttgtgcgacg cggagatcat cacgccgaac tcggcgtcgt
     2041 aggcgctggt caggtacgcc accgcgggcg tcggcaggac cccgacgcgc agcgcgtcca
     2101 cgccttcgct ggtgataccg gcgatcaccg ccgcctcgat catctcgccg ctggcccgcg
     2161 gatcgcggcc gatgaccgcg acgctgcgtc ccgcggaggt ggcgccgagc cggcgcgccg
     2221 acgcggagcc gatggccagc gccagctccg cggtgagctc gcggttggcg accccgcgga
     2281 cgccgtcggt gccgaacagt cgacccatgc cgacaaactt ctcacagtcg gcgcccgaag
     2341 gcacaaccga cgagctcatc agccgtcttg ccaccgaatc gcgatcagtg cggtacccgg
     2401 agacgggtca ccgcccgcac cccagttggg atgcgggcgg tgatttccga atcagctggc
     2461 cggggtcaag cccgacgtcg gcgtcatcgg cgccgtcggt ggcgcctccg actgcgagcc
     2521 attcggctgg tcggcgatgt tctcggcgtt cggcccggcc gccggcgcgg tgttggcgtt
     2581 cggcccggcc gccggcgcgg tgttggcgtt cggcgtgttc gtcggcacgt tgttcgcgtt
     2641 cggcgaagtc accggg
//
LOCUS       DASWBF010000022         6311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_67711, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000022 DASWBF010000000
VERSION     DASWBF010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67711"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..1358
                     /locus_tag="VF874_00605"
     CDS             477..1358
                     /locus_tag="VF874_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HEX9174640.1"
                     /translation="MAVIEEGSFDAAARRLQITPSAVSQRIKALEQQVGQVLVKRERP
                     CVPAASALPLVRVAAQMALLEAEAIAQLRGGEQHATRISLAVNADSMATWFTGVFGML
                     GDDVLVEVHIEDQDHSARLLRNGAVMGAVTTERRPIPGRRVHPLGAMRYVPVAGRAFA
                     TRFLPSGFTAAAVAHAPTVSWNRDDALQEDFVRKVFRRSVAMPVHYVPTVEGNLAAIQ
                     AGLGWGMFPDALPPHGQLVRISDDHLDVPLYWQCWKLDSVLVAKVTDAVRSAAAKLTS
                     PQSRVRGRRTATSSAGR"
     gene            complement(1436..2647)
                     /locus_tag="VF874_00610"
     CDS             complement(1436..2647)
                     /locus_tag="VF874_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9174641.1"
                     /translation="MSASDEVWIIGIRMTKFGKHLDLDAVDLAAEAATGALADAGVTM
                     ADIGVLAAGNLMNASASIGQQLQKQIGQTGIPVYNVANACATGATALRTAIMAVKAGE
                     VDYGLAVGVEKLAGAGLLGAGGKKRGDAQTWTPSGRYGAVTPVDGRIGTEAMPGVFAQ
                     IGMEYDHKYGGASFELFAKISEKNHAHSTLNPLAAYQKKFTLDEIMNDVMIAYPNTRP
                     MCSANCDGAAAAVVCNGETLKSLSQQQRRRAVKVSASVLTTDPYEEGCQVLPNVNTLT
                     RNAAATAYEQAGVGPGDLDLVELHDCFATAELVHYDNLMLCEEGGAAEFFNSGATWRD
                     GSTPVNVSGGLQSKGHPIAATGIANIWEICHHLRGEAKDRQIENATVGLAHVIGLGSA
                     CGVHILERSAA"
     gene            complement(2664..3062)
                     /locus_tag="VF874_00615"
     CDS             complement(2664..3062)
                     /locus_tag="VF874_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX9174642.1"
                     /translation="MAKQIPMVDYLVLDDGEPHLVAQQCASCGARFFDRRNACAKCFA
                     TEFTTVPIATEGTVRAFTIVTFAAPGIPTPFVASVVDCDGTQVRANLTNIEPDPESIR
                     TGMKVRLATYSLGEDSEGTEAVGFVFEPAE"
     gene            complement(3101..4336)
                     /locus_tag="VF874_00620"
     CDS             complement(3101..4336)
                     /locus_tag="VF874_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX9174643.1"
                     /translation="MAGPMSLEVAGRQVAVTHPDKVVFPAADGHPARTKLDVIRYYLS
                     VADGALRGVADRPMILKRFVKGLTQEAIFQKRAPEKRPDWIDVAELKYASGTSAKEAV
                     IHDAAGLAWAVNLGCIDLNPHPVRAGDLDHPDELRIDLDPVPGVPWQQIVDVALVVRD
                     VLADNGLTGWPKTSGSRGFHIYARIAPRWPYRHVRLAAETVAREVERRAPDLATSRWW
                     KEERQGVFVDFNQNAKDRTTASAYSVRATPGARVSTPLFWDEVSRRHAEAFTVDTVPA
                     RFADIGDPWAGMDDASGSLDALLELAERLGPAEKPPKKGTGRRESTKPLIEVARTKTK
                     DEAMAALDTWRSRHPEAAKKLEPADVLVDGMRGPSSIWYRIRINLQHVPEDQRPPQED
                     LLADYNPWEGYSGPQWMRG"
     gene            4446..6125
                     /gene="fadD2"
                     /locus_tag="VF874_00625"
     CDS             4446..6125
                     /gene="fadD2"
                     /locus_tag="VF874_00625"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD2"
                     /protein_id="HEX9174644.1"
                     /translation="MPTLNPADLVAQAAAKAKQYAERGAAELHYVRKMFESGALKLES
                     PKMVSAMAADIARWGEIGMIPSVNARRTPHRVAVIDDEGALTFSELDAAANAAANAMI
                     AMGVKGGDGVAILGRNHRWFIIANYACARVGARIILLNTEFSARQIAEVADREGARLL
                     IYDDEYTQAVSEANFELGRLRALGHNPDAEEPSGSTDETLAEVIAHSSKEPAPKPTKQ
                     SSLVILTSGTTGTPKGATRHTPPTLAPIGGVLSHVPFKAGEVTSLPSPMFHALGYLHA
                     TIAMTLGSTLVMRRKFRPLTVIEDIEKYRATALVVVPVMLSRILDALDKADRSYDLSS
                     LRIVFVSGSQLGAELATRATKRLGPVIYNLYGSTEVAFATIARPQDLDVNPSTVGPVV
                     KGAKVRILDEHGNDVPQGQVGRIFVGNTMPFEGYTGGGGKQIIDGLLSSGDMGYFDDN
                     GLLYVTGRDDEMIVSGGENVFPAEVEDLISGHPEVIEATALGVEDEEWGARLRAFVVK
                     QEGSGVDEDAIKLYVREHLARYKVPREVVFLDELPRNPTGKILKRELRKID"
BASE COUNT         1051 a         2111 c         2067 g         1082 t
ORIGIN      
        1 aaccgacgct cggtcaccga gttctcaaca gggcgcacga cccaggtgcg gtacccggcc
       61 acccacacac tcaacaggaa agaagtcagc gcatcggctg ctccggtcgc agtcccgagg
      121 cgatggccgg gccccagcga actcagtatg aatgtccatc cgctgcaccc cggagcgacg
      181 ctagcggcgg acaatttggc gcgtcatcgg tggccgccaa ggcgtcgaac gtggcacatc
      241 gaatccgcgc gatggccagc gaatagcacg cgcgaatcgc tccggctgcg atcgcatccc
      301 ccggtccaac tcgtccgaga agcagcggca ccggtccacg gttgtgtggg ttggcatccc
      361 gccgcgtagg aactgaacca ctccgtgaga caaaaccggg tcgcaatcgc gtgagacgtc
      421 caagagaccc gcaggtcacc gggcgccgac gatgtcaccg agggtgagac cctacagtgg
      481 cagtaataga agagggcagt ttcgacgcgg ccgcgcgccg gctacagatc actccttcgg
      541 cagtcagtca gcgaatcaag gcgctcgaac agcaggtcgg ccaggtgctc gtgaagcgcg
      601 aaaggccctg tgtgccagcc gcatcagcgc tgccgctggt gcgggtggcg gcccagatgg
      661 cgctgctgga ggctgaagcg atcgcccagt tgcgtggcgg agagcagcac gccacccgaa
      721 tatcgttggc tgtcaacgcc gattcgatgg ctacctggtt caccggcgta ttcggcatgc
      781 tcggcgacga tgtgctggtc gaggtgcaca tcgaggatca ggatcactcg gcccgactgc
      841 tgcgcaacgg cgcggtgatg ggcgcggtga ccacggaacg caggccgatc ccgggccgcc
      901 gggtgcaccc actcggtgcg atgcgctacg taccggtggc gggccgcgcc ttcgcgacgc
      961 ggtttctgcc cagcggcttc accgccgcgg cggtcgccca tgcgccgacg gtgtcgtgga
     1021 accgcgacga tgcgttgcag gaagatttcg tgcgcaaggt ttttcgtcgc agcgtcgcga
     1081 tgccggtgca ctatgtgcct acggtggagg gcaatctcgc tgccatccag gccgggcttg
     1141 gctggggcat gtttccggac gcactgccgc cgcacggtca gcttgtcagg atcagcgacg
     1201 accacctcga cgtgccgctg tactggcagt gctggaagct ggactcggtg ctcgtcgcca
     1261 aggtcaccga tgccgtgcgg tcggccgccg cgaaactcac ctcgccacaa tctcgtgttc
     1321 gcggacgtag aacagcgacg agttcggcag gtcgatgaac ctgcgctcgg gctggtatgc
     1381 gcgcgccgac cgcaaggggt ggaccaacat gcccaacgaa gcctgagcgc ccgacctacg
     1441 ccgccgaccg ttcgaggatg tgaacgccgc atgccgagcc caggccgatg acgtgggcga
     1501 gaccgaccgt cgcgttctcg atctgacggt ctttcgcctc gccccggagg tggtggcaga
     1561 tctcccagat gttggcgatg ccggtcgcag cgatcgggtg gcccttggac tgcaggccgc
     1621 ccgacacgtt caccggcgtc gatccgtcgc gccacgtggc acctgaattg aaaaactctg
     1681 cggcaccgcc ctcttcgcac agcatcaggt tgtcgtagtg caccaactcg gcggtcgcga
     1741 agcaatcgtg caattcgacg aggtcgagat cgcccgggcc cacgcccgct tgttcgtacg
     1801 cggtggcggc ggcgttgcgg gtgagtgtgt tgacgttcgg caacacctgg cagccttcct
     1861 cgtaggggtc ggtagtcagt accgacgccg acactttgac cgcccgccgc cgctgctgct
     1921 gcgacagcga cttgagcgtc tcgccgttgc acaccaccgc ggccgcggct ccgtcgcagt
     1981 tggccgagca catcggccga gtgttcgggt aggcgatcat gacgtcgttc atgatctcgt
     2041 ccaatgtgaa cttcttctgg taggcggcca gcggattgag cgtcgaatgc gcgtggttct
     2101 tttcactgat cttcgcgaag agctcgaagc tcgcgccgcc gtacttgtgg tcgtactcca
     2161 tgccgatctg cgcgaagacg cccggcatcg cctcggtacc gatccgcccg tcgaccggcg
     2221 ttaccgcgcc gtagcgacct gacggcgtcc atgtctgcgc atcccctctc ttcttcccgc
     2281 ccgcgccgag aagacctgcg cctgctagtt tctcgacgcc gaccgccagg ccgtagtcaa
     2341 cttcgccggc cttgacggcc atgatcgccg ttcgcaatgc cgtcgcaccg gtggcgcacg
     2401 cattggcgac gttgtacacc ggtatgccgg tctgaccgat ctgcttctgc aactgctggc
     2461 cgatgctggc gctcgcgttc atcaggttgc ctgccgcgag cacgccaatg tcggccatgg
     2521 tcaccccggc gtcggcgagg gcccccgtgg cagcctcggc ggccaggtcg acagcgtcga
     2581 ggtcgaggtg ctttccgaac ttggtcattc ggatcccgat gatccagacc tcgtcgctgg
     2641 cgctcatcgt ctgttctcct tgatcactct gcgggctcga acacgaaccc gacggcctct
     2701 gtgccctcgg aatcttctcc gagtgaatac gtcgcgagcc gcactttcat cccggtgcgg
     2761 atgctttccg gatccggctc gatgttcgtc aggttggccc ggacctgggt gccgtcgcag
     2821 tcgacgacgg acgccacgaa cggggtgggg atgcctggcg cggcgaatgt gacgatcgtg
     2881 aacgctcgca ccgtgccctc cgtcgcgatg gggacggtcg tgaactctgt ggcgaagcac
     2941 ttggcgcagg cgttccgccg gtcgaagaag cgggcgccgc agctggcgca ctgctgggcg
     3001 acgagatgcg gctcgccatc gtcgagcacc aggtagtcga ccatcggtat ctgcttggcc
     3061 atctgcacct ccgttgccgg tccttcgacc gtagcggtct ctaaccgcgc atccactgcg
     3121 ggccggagta accctcccac gggttgtagt cggcgagcag atcctcctgc ggcgggcgct
     3181 gatcttcggg cacatgctgc aggttgatcc ggatccggta ccagatcgag ctcggcccgc
     3241 gcatcccgtc gaccaacacg tcggcgggct ccagcttttt cgccgcctcg gggtgccgcg
     3301 agcgccacgt gtccagtgcg gccatcgctt cgtccttcgt cttcgtccgg gcgacctcga
     3361 tcagcggctt cgtcgactct cgtctgccgg ttcccttttt agggggcttc tccgctgggc
     3421 ccagccgctc cgccaactcc agcaatgcgt ccaacgaccc cgaggcgtcg tccatcccgg
     3481 cccacgggtc gccgatgtcg gcgaaccgcg ccggaaccgt gtccaccgtg aaagcttctg
     3541 cgtgacgacg cgatacctcg tcccagaaca gcggcgtcga gacccgcgca ccgggggtgg
     3601 cgcgcaccga gtacgccgac gccgtcgtgc ggtctttggc gttctggttg aagtcgacga
     3661 agacgccctg gcgttcttcc ttccaccatc ggctagtggc caggtccggt gcgcgccgct
     3721 ccacctcgcg ggcaaccgtt tccgccgcca gccgcacgtg tcgatacggc caccgcggcg
     3781 cgatgcgcgc atatatatgg aagccccgtg accccgaagt cttcggccag ccggtcagcc
     3841 cgttgtcggc cagcacgtcg cggaccacga gcgcgacgtc gacgatctgc tgccagggca
     3901 cgccgggcac ggggtcgagg tcgatgcgca gctcgtcggg gtgatcgagg tcgccggccc
     3961 gcaccgggtg cgggttgagg tcgatgcacc cgaggttgac cgcccacgcc agtcctgcgg
     4021 cgtcgtgaat caccgcctcc ttggccgacg tgcctgacgc gtacttcagc tcggcgacgt
     4081 cgatccagtc cgggcgcttc tcgggtgcgc gcttctggaa gatcgcctct tgggtcagcc
     4141 ccttgacgaa ccgcttgagg atcatcggcc ggtcggcgac gccgcgcagc gcaccgtcgg
     4201 ccaccgacag gtagtagcgg atcacgtcga gcttggtgcg ggccggatga ccgtccgccg
     4261 cggggaagac gaccttgtcg gggtgcgtga cagcgacctg ccggccggcc acctcgagcg
     4321 acatcgggcc ggccataccg ccatcgtagg aagctatgcc gggttgacgc ggaacacggg
     4381 atagcgggca gcgactttct cctactcaga cctggctgag atccccgtca cataaggtaa
     4441 tgcccatgcc cacgctgaac cccgctgacc ttgtcgcaca ggccgctgcg aaggccaagc
     4501 agtacgccga gcgcggcgcg gccgaattgc actacgtccg aaaaatgttc gagtccggcg
     4561 cgctcaaact ggagtcgccg aaaatggtga gcgcgatggc cgcggacatc gcgcgctggg
     4621 gcgaaatcgg gatgatcccg tcggtcaacg cgcgccgcac accgcaccgc gtcgcggtca
     4681 tcgacgacga gggcgccctc actttcagcg agctcgacgc cgccgccaac gcggccgcca
     4741 atgcaatgat cgcgatgggt gtcaagggcg gcgacggcgt cgcgatcctg gggcgcaacc
     4801 accgctggtt catcatcgcg aactacgcat gcgcgcgggt gggcgcccgc atcatcctgc
     4861 tcaacaccga gttctccgcc cggcagatcg ccgaggtcgc cgaccgcgaa ggcgcgcggc
     4921 tgctgatcta cgacgacgag tacacgcagg cagtgtcgga ggcgaacttc gaactgggca
     4981 ggctgcgcgc gcttgggcac aatcccgacg cggaggaacc gtcgggcagc acggacgaga
     5041 cgctggccga ggtgatcgcg cacagtagca aggaacctgc cccgaagccg acgaagcagt
     5101 cctcgctggt gatcctcacc agcggcacca ccgggacacc gaagggcgcc acccggcaca
     5161 ccccaccgac gctggccccg atcggcggtg tcctctcgca cgtgccgttc aaagccggag
     5221 aagtgacgtc gctgccgtcg ccgatgttcc acgcgctcgg atatctgcac gcgacgattg
     5281 cgatgacgct gggctcgacg ctggtcatgc gccgcaagtt ccgtcccctc accgtcatcg
     5341 aggacatcga aaagtaccgt gccacagcgc ttgtcgtggt gccggtgatg ctgtcccgca
     5401 ttctggacgc gctcgacaaa gccgaccgca gctacgacct gtcgtcgctg cgcatcgttt
     5461 tcgtctccgg ctctcagctc ggcgcggagt tggcgacccg ggcgacgaag aggctcgggc
     5521 cggtgatcta caacctgtac ggttccaccg aggtcgcgtt tgcgacgatc gctcgcccgc
     5581 aggatctcga cgtcaacccg tcgacggtcg ggcccgtcgt caagggcgcg aaggtcagga
     5641 tcctcgacga gcacggcaac gacgtcccgc agggacaggt gggtcgcatc ttcgtcggca
     5701 acacgatgcc attcgaggga tacaccggcg gcggcggaaa gcagatcatc gacgggcttc
     5761 tgtcctcagg cgacatgggc tacttcgacg acaatggatt gctgtacgtc accggccgcg
     5821 acgacgaaat gatcgtctcc ggcggagaaa acgtgttccc ggccgaagtc gaagacctga
     5881 tcagcggtca ccccgaagtc atcgaggcca ccgcgctggg tgtcgaggat gaagagtggg
     5941 gggcccggtt gcgggctttc gtggtgaagc aagagggttc gggcgtcgac gaggacgcga
     6001 tcaagctcta cgtgcgcgag cacctggccc gctacaaggt gccgcgcgag gtggtcttcc
     6061 tcgatgagct gccgcggaac ccgaccggca agatcctcaa acgcgaactt cgcaagatcg
     6121 actaacgcgt ttgcgccgaa accgaatttc gggtcgtgat ttcggcgatt cgacagccct
     6181 ggcttcacgt tcggcgcagt ggcaacgaca gcggaggctt acgggtcgcg gggcaggccc
     6241 agcagccgct cggcgatgat gttcagctgc acctcggagg tgccgccgta gatcgtcgtc
     6301 gcgcggctga a
//
LOCUS       DASWBF010000023         6611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1999, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000023 DASWBF010000000
VERSION     DASWBF010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..383)
                     /locus_tag="VF874_00630"
                     /pseudo
     CDS             complement(2..383)
                     /locus_tag="VF874_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009937679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(411..1028)
                     /locus_tag="VF874_00635"
     CDS             complement(411..1028)
                     /locus_tag="VF874_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX9174645.1"
                     /translation="MRRRLLAIGAAAVATLTTLTGCSGSSPEAGTSPAGTTTSAAAQP
                     HNQADAMFAHHMIPHHQQAIEMSDIVLGKQGIDPRVVTLANQIKAAQGPEIQQMQSWL
                     SQWGEPTMMPMPSSMMPGHDMGDMPGTSGMMSAEDMAALKNAQGVEASKLFLTHMIAH
                     HQGAIAMAQDEIKSGQYPPAIAMSQSIVTSQQKEIDEMQAILASL"
     gene            1109..1762
                     /locus_tag="VF874_00640"
     CDS             1109..1762
                     /locus_tag="VF874_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9174646.1"
                     /translation="MGLTQKLLSHHHHDDGSGGVITHPRGYELFAGAWFLGQRAWTWD
                     RLVALAGPQPGDRVLDVGSGTGYFARRIAPVVEPGGSVVGIDPSQPMLVYAAAHAPAN
                     CTFQAAGAEDVPFGDASFDLVVSSLAFHHFPVDRRADAVREMFRVLRPGGRLFIADFR
                     PPRGPVLNRIVAAVTAHAMAHDNAHEISELLRDCGFTVTGTGRTRLMHYVKALRPQG"
     gene            complement(1770..2036)
                     /locus_tag="VF874_00645"
     CDS             complement(1770..2036)
                     /locus_tag="VF874_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX9174647.1"
                     /translation="MVGDEDSIAAVLNRLRRAQGQLAGVIAMIEQGRDCKDVVTQLAA
                     VSRALDRAGFKIVATGLRECVSGAQPKGGQMTEAELEKLFLALA"
     gene            complement(2096..2974)
                     /locus_tag="VF874_00650"
     CDS             complement(2096..2974)
                     /locus_tag="VF874_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9174648.1"
                     /translation="MTIALTVLLAVFVGISLGLLGGGGSILTVPLLVYVAGLDPKPAI
                     ATSLLVVGVTGAIGAIGHARAGRVWWRTAAAFGLAAIVGAYAGGRLARFVPGTILMIA
                     FALIMIVAAAAMLRGRTASTATPGRQHLPMVKIAVLGTAVGMLSGLVGAGGGFLLVPA
                     LALLAGLPMTAAVGTSLVVIAMQSFAGLAAHLADEPIDWRLAAVVTAAAVLGAILGGR
                     LSSMVDANALRTMFGWFVLVMASVILAEEAGPEVGLTAAGITLCAAGVYTICRRTTHC
                     PLRRLASRAQAGAAAA"
     gene            3118..4494
                     /locus_tag="VF874_00655"
     CDS             3118..4494
                     /locus_tag="VF874_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9174649.1"
                     /translation="MILEQYYIECLSQASYLIGDETTGRAVVVDPRRDISDYLADAKR
                     YGLTIEGVINTHFHADFVSGHLELVEATGAWIGFGACAETDYPIRRLADGEHISLGRV
                     DLQIVSTPGHTWESISVVVREHPGAVPTAVLTGDSLFIGDVGRPDLVNIGDSSTDDLA
                     RAMYHSIHDKLFALPDQVVVMPAHGAGSSCGKNLSTELTSTIGEQRRTNPSVQPMSED
                     AFVELITRGQPAAPAYFSVDAAMNKHVHPLLEQNREIPALSPSQLRAAMDAGIRVLDA
                     RSVEDFAAGHLRGSVNVGFDGRFAETGGMVADVDEKIALVTYPGEEQLAAMRLARIGS
                     DNAVGYLNVDHDGVFPAELSGLLRTAPRVTAAELDRMLARDAVTLIDIRNPGELDAGV
                     IPGAVHIPLAQLRARLGEVPAGKPIVVHCAGGWRSSVAASLLRAAGLEDVSDLAGGYT
                     GWAQAHAA"
     gene            4672..4932
                     /locus_tag="VF874_00660"
     CDS             4672..4932
                     /locus_tag="VF874_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX9174650.1"
                     /translation="MMYGWGWGWGWGGWIVVTLLMVAFWAAVITGIVLAIRYTSGDGQ
                     RSQARGAPTAEDVLAERFARGDIDEEEYRRRLTLLREHRSTG"
     gene            4929..5495
                     /locus_tag="VF874_00665"
     CDS             4929..5495
                     /locus_tag="VF874_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HEX9174651.1"
                     /translation="MTARTIVAVGAASVVLGVATTGLIALASGGPPPASASCEAPALA
                     GAVVNVSLTDMGDMMGPGMMGPSGHMRGMGMMRITADPATVSAGQISLRANNFGALTH
                     EVVILPLAPGQWPGQRAIGADGEVDESGSVGEASRTCGADEGEGILPGASGWTTLTLP
                     PGRYELLCNIAGHYGAGMYTELDVTGNR"
     gene            5616..6307
                     /locus_tag="VF874_00670"
                     /pseudo
     CDS             5616..6307
                     /locus_tag="VF874_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815921.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            complement(6416..>6611)
                     /locus_tag="VF874_00675"
     CDS             complement(6416..>6611)
                     /locus_tag="VF874_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9174652.1"
                     /translation="EVLPRRWVVERTLSWLVRWRRLDRDYERLPTHSEAMVKWAMIGL
                     MARRLAPPPGRRPWQPADAP"
BASE COUNT         1103 a         2181 c         2249 g         1078 t
ORIGIN      
        1 actatgacgc gggggcgacg gccgcgatca gcgcgtcggg gtccgtcacg ctcacccaga
       61 gcgtgcgcag cgtgatcggg acgcccatga catgtgcgcg cgcaccggcg ggtcgatggt
      121 caactccacc atgcctttcg acgatccgtt gaccagccac cggccccgcc agccgtgtac
      181 cccccaggcg tacaccctgt cgttgttcgg cgccgctgtc ttgatggagc tcagcgggat
      241 cgtcgcccgg aaccccaccc catcgcgacg cggaggttgc cgtcctcgat ccggacctcg
      301 ctttgtccgg ggccgaggcc gagaggagcg gacagcggca ggaaccatcg ttcgtaacga
      361 agctcggtgg gcacgaccgc cagagtactt cgcgcgacac gcgcctggac ctacagcgac
      421 gccaggatgg cctgcatctc gtcgatctcc ttctgctggc tggtgacgat cgactgggac
      481 atcgcgatgg ccggcgggta ctgcccggac ttgatctcgt cttgcgccat ggcgatcgct
      541 ccctggtggt gcgcgatcat gtgcgtcagg aacagcttgc tggcctcgac gccttgggcg
      601 ttcttcaaag cggccatgtc ctcggcggac atcatcccgc tcgtgccagg catgtcgccc
      661 atgtcgtgcc cgggcatcat gctgctgggc atcggcatca tcgtcggctc gccccattgg
      721 ctcagccacg actgcatctg ttgaatctcg ggtccttgag ccgccttgat ctgattggcc
      781 agcgtcacca cccgagggtc gattccctgc ttgcccagca cgatgtcgct catctcgatg
      841 gcctgctgat gatgcggaat catgtggtgg gcgaacatcg cgtcggcctg gttgtgcggc
      901 tgcgccgccg ccgaggtcgt ggtgccagcg ggtgaggtgc cggcctcggg agaggagccg
      961 ctacacccgg tcagggtggt cagagtcgcc accgccgcag caccgatggc cagcaaccgt
     1021 cgcctcatta gaatcgtcct tccgtaacta cgagtcggat tacgtccgcc tattgatata
     1081 taccctatgg gggtatagaa agacagtcat gggactgact cagaagctgc tgtcgcatca
     1141 ccaccacgac gacggctccg gcggggtgat tacccatccc cgcggctatg aactcttcgc
     1201 cggcgcctgg tttcttggcc agcgcgcgtg gacttgggac cgtcttgtcg cgttggcggg
     1261 cccccaacct ggcgatcgtg tgctcgacgt cggaagtggc acagggtatt tcgcacgacg
     1321 gatcgcgccg gtggtggagc ctggcggatc ggtggtgggc atcgacccgt cgcagcccat
     1381 gctcgtctac gccgccgcac atgccccggc caactgcacg ttccaagctg ccggcgctga
     1441 ggacgtgccg ttcggcgacg cgtcattcga cctggtggtg tcgagcctgg cgtttcacca
     1501 cttcccggtc gatcgccggg ccgacgcggt gcgcgagatg ttccgcgtgc tgcggcccgg
     1561 cggtcggctc ttcattgccg acttccgccc gccgcgcggt ccggtgctca accggatcgt
     1621 cgcggccgtc accgctcacg cgatggccca cgacaacgct cacgagatca gcgaattgct
     1681 ccgcgactgc gggttcaccg tcacgggcac ggggcgtacc cggctgatgc attacgtcaa
     1741 agcgctacgg ccacaaggat agtcgttgat tacgcgaggg cgaggaacaa cttctccaac
     1801 tcggcttcgg tcatttggcc gcctttgggc tgagccccgc tgacgcattc cctcagtccg
     1861 gtggccacga tcttgaaacc ggcgcggtcc agtgcgcgcg aaaccgcggc gagctgagtg
     1921 acgacgtcct tgcagtcacg tccctgctcg atcatcgcga tcacgccggc gagctggcct
     1981 tgggcccgtc gcaatcggtt caggactgcg gcgatgctgt cttcgtcacc aaccatggtg
     2041 cttcccttcg atcgtgcgct gacatctatg gtaccggcgg gggtatcgac ggcagtcatg
     2101 ccgcggcggc gccggcctgc gctctgctgg ccagcctgcg tagaggacaa tgtgtcgtcc
     2161 gtcggcagat ggtgtaaacg cctgcagcgc acagggttat gcccgcagcg gtcaggccca
     2221 cctcggggcc cgcttcctcg gcaaggatga cggaggccat caccaacacg aaccagccga
     2281 acatcgttcg taatgcgttt gcgtcaacca ttgagctgag ccgaccgccg aggatcgcac
     2341 cgagaaccgc ggccgccgtc accaccgcgg ccagccgcca gtcgatcggc tcgtcggcca
     2401 ggtgcgcggc caagccggcg aaggactgca tcgcgatcac caccaacgac gtgcccacgg
     2461 cggcggtcat cggcaatccc gcgagcaagg ccagcgccgg caccagcagg aaaccgccgc
     2521 cggcgccgac cagcccgctc aacattccca ccgcggtgcc aagcaccgcg atcttgacca
     2581 tcggcagatg ctgccgcccg ggtgtcgcgg tggacgccgt gcggccccgg agcattgcgg
     2641 ctgcggcgac gatcatgatc agagcgaacg cgatcatcag gatcgtgccc gggacgaagc
     2701 gggccaggcg gccgccggcg taagcgccga cgatcgccgc aagaccgaag gccgcagccg
     2761 tacgccacca gacccggcca gcgcgggcgt ggccgatcgc gccgatcgcg ccggtcacgc
     2821 ccaccacgag cagcgacgtg gcgatcgcgg gcttcgggtc gagtcccgcc acgtagacca
     2881 gaagcgggac agtgaggatc gacccgccgc cgcccaacag gccgagcgaa atgccgacga
     2941 aaaccgccag cagcacggtc aacgcgatcg tcatcgtcac ctgaaacctc cgctcccgct
     3001 catacccacc tgggtataaa ctcgctggga acaaatatac cccagggggt acttgacata
     3061 ccagtagggg tatcagccat tatgggtggt accaagacgg aagacgggga agaggccatg
     3121 atcctcgagc agtactacat cgagtgcctg tcgcaggcgt cgtatctgat cggcgacgaa
     3181 accaccgggc gcgcggttgt tgtcgacccg cgccgcgaca tcagcgacta cctcgccgat
     3241 gcgaagcggt acggactgac gatcgaagga gtcatcaaca ctcacttcca cgccgatttc
     3301 gtgtccgggc atctggagct ggtcgaggcg accggggcgt ggatcggctt cggcgcgtgc
     3361 gcggaaaccg actacccgat ccgtcgactg gcagacggtg aacacatctc gttgggccgg
     3421 gtcgacttgc agatcgtttc gacgccgggc cacacctggg agtcgatcag cgttgtcgtg
     3481 cgcgagcatc ccggggcagt gccgaccgcg gtcctgacgg gtgactcgct gttcatcggc
     3541 gacgtgggcc gcccggacct ggtgaacatc ggcgacagtt cgaccgacga tctcgcccgc
     3601 gcgatgtacc acagcattca cgacaagctg ttcgcgctgc ccgaccaggt cgtcgtgatg
     3661 cccgcgcatg gcgccgggtc gtcgtgcggc aagaacctgt ccaccgagct gacatcgacg
     3721 atcggtgagc agcgccgcac aaacccgtca gtgcaaccga tgagcgaaga cgcgttcgtc
     3781 gagctgatca cacgggggca accggccgca ccggcgtatt tctccgtcga cgcggcgatg
     3841 aacaaacacg tccacccgct gttggaacag aatcgcgaaa tccctgcgct cagcccgtcg
     3901 caactgcgcg cagcgatgga cgccggaatc cgcgtgctcg acgcccgatc ggtggaggat
     3961 ttcgccgccg ggcaccttcg gggatcggtg aacgtcggct tcgacggccg gttcgcggag
     4021 acgggcggga tggtggccga cgtcgacgag aagatcgcgc tcgtcacgta tcccggcgag
     4081 gagcagctcg cggcgatgcg gctggcacgc atcggttctg acaacgccgt cggctacctc
     4141 aacgtcgacc atgacggcgt gttccccgcc gaactgtcgg gtctgctgcg gaccgcgccg
     4201 cgggtgacgg ccgcagaact ggaccggatg ctggcgcggg acgcggtcac gttgatcgac
     4261 atccgcaacc ccggagagct cgatgcgggc gtgatccccg gagcggtgca catcccgctc
     4321 gcgcagctgc gggcgcggct gggcgaggtg cccgccggca agccgatcgt cgtgcactgc
     4381 gccggcggct ggcgctcgag cgtggcggcg tcgctgctgc gggcagccgg actggaggac
     4441 gtgtccgact tggcgggcgg ctacaccggc tgggcgcagg cgcacgcggc ctgactcaca
     4501 cgccgcctaa tcacaaagga gagaagcaat gtgttacccg gtgcagtgtc cacgctgcgg
     4561 caagaccggt tggggaggct gcggcgagca cgtcgacagc gtgatgcgtt cggtgcccgc
     4621 atccgaaaga tgccggtgcg ctgtgcgcgc tgactcggag gtcgtaccac gatgatgtac
     4681 ggctggggct ggggctgggg ctggggcggc tggatcgtgg tgacccttct gatggtcgcg
     4741 ttttgggccg cggtgatcac ggggatcgtc ctggccatcc ggtacacgtc gggagacggg
     4801 cagcgcagcc aggctcgggg ggcgccgacg gccgaagacg tgctcgcaga acgattcgcg
     4861 cgcggtgata tcgacgagga ggaataccgc cggcgcctga cgttgttgcg ggagcaccgg
     4921 agcacgggat gaccgcccgg acgatcgtgg cggttggggc cgcgtccgtc gtgctcggcg
     4981 tggcgacgac gggtcttatc gctttggcct cgggcggtcc gccgccggcg tcggcgtcgt
     5041 gcgaagcgcc tgcgctggcc ggcgccgtgg tgaacgtgag tcttaccgac atgggcgaca
     5101 tgatgggtcc cgggatgatg ggaccctccg gacacatgcg cggcatggga atgatgcgta
     5161 tcacggcgga cccggcgacc gtttcggcag ggcagatttc gttgcgggcc aacaacttcg
     5221 gcgctctcac tcacgaagtg gtgatcctgc cgctggcccc gggtcagtgg ccgggccagc
     5281 gggccatagg tgccgacggc gaggtcgacg agtccggcag tgtcggcgaa gcatcgcgga
     5341 catgcggcgc cgacgaaggg gagggcattt tgcccggggc gagcggctgg accaccctca
     5401 cgctgccgcc gggacgctac gaattgctgt gcaacatcgc cggccattac ggtgcgggca
     5461 tgtacaccga gctcgacgtc accggcaacc gctaactcgc gtgagcgtca tgcgggggaa
     5521 gtaaatcggt cgggctccat cgcgaacttg cccagcacag cggagagaac agaaaagata
     5581 atcgctgccc gcgtacgtca gccgtccaac ggcccgagat cgaggagttt cttactggca
     5641 gccatgccgc gccgtcgcag tcgcatcgcg ccctgcgcac cgtgttgttc accgacatgg
     5701 tcgcctccac gcaacacgcc gcggcgacag gcgacgagcg gtggcgtgcg gtgctgcacc
     5761 gctttggtga gatcaccgcc gaactcacgg aacgattcgg cggcacggtg gtcaagaaca
     5821 ccggcgacgg ccacctcacc acgttcgacg gcccgacgca ggccatccgc tgcgccgagg
     5881 ccctgcgtgc tgacgccgaa acactgggca tccagatccg cgctggcatc cacaccggcg
     5941 agtgcgaact gctggacgac gacctcgggg gctcgcggta catatcgcgg cgcgcatcct
     6001 cggccaggcc ggccccggtg aaatcctggt ctcgcgcacc gtgcgtgacc tgatcgtcgg
     6061 gtcgggcacg ggcttcgagg accgcggcag cgtcgagttg cgtggtgtgc ccggcacctg
     6121 gcaactgctg ggggtcgatc gccacggagc gcaggcggga tcggccgagg cggaactggc
     6181 gtcaacgccc acccccggcc cgcgcaccgc gatgcgccgg tcggaccgcg ccgtggcgct
     6241 gatggccaga cgcacaccat ggatcctgcg cggtatggcc cgcctcgccc cgacggttcg
     6301 gagctgaaca gctaccggag tcaacgcgct cggcggtgcg atttgctgtt ggggccgacg
     6361 tagccgctgt gtttagacgc atcagtgcat tacttagaac gtgttggaaa ggggtttacg
     6421 gggcatcggc cggttgccag ggccgtcgtc cgggcggggg cgccaggcga cgtgccatga
     6481 ggccgatcat ggcccatttg accatggctt cggaatgggt gggcaggcgt tcgtagtcgc
     6541 ggtcgagtcg tcgccagcgc accagccagg acagggttcg ttccaccacc caacggcgcg
     6601 gcagaacctc g
//
LOCUS       DASWBF010000024         5004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_67790, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000024 DASWBF010000000
VERSION     DASWBF010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67790"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VF874_00680"
     CDS             complement(<1..152)
                     /locus_tag="VF874_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9174653.1"
                     /translation="MARTSSETQSSTHAQSKWLSKSAAQTRGSDTEEVQFHYDISNEF
                     FKLWQDP"
     gene            complement(270..1763)
                     /locus_tag="VF874_00685"
     CDS             complement(270..1763)
                     /locus_tag="VF874_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9174654.1"
                     /translation="MEQLSGLDAAFVYAETAGAAHVTYFAIYDPSTAPGGVVTFDDVA
                     AHIRSRLGADRIFRTVLARVPFDLDHPYWVRDENFELSRHLHHLTLPRPGGWRQLCEQ
                     ISQLHTERMDLSRPPWEGYFIEGLGRIKGVPKGAFAVCFKVHHSAVDGVTGLSLVDAL
                     HDLTPETAPPPDDDWTPEPRPSSLNLLARTAVTYARRPAQFASAARHTLPLLSKVPGA
                     LTRRLPLGRQEQEDERPSHQVPASRFNAHIDTSRNFDARSYDLDAVLKARTLVPHATV
                     NDVVVAGIGGALRRYLQEKDELPDTSLVTAVAIAVHTDPNRRGVNQMGVVRLPLGTDL
                     EDPVARLGVVHDASTRSKAFNEEVAPRVLEYAEFLPGEVLVPALRLAMAAGLGRLAEN
                     SRISNTLVSTMRGPDFPVYLVGARMLGGYAFSPFTQGGGLIHNVISYCGRVVVSINGC
                     PAVLPDIEHYGDCMDAAFAEVMSAAKTSAPRKTPRRSSATGEDHQRR"
     gene            complement(1851..3263)
                     /locus_tag="VF874_00690"
     CDS             complement(1851..3263)
                     /locus_tag="VF874_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX9174655.1"
                     /translation="MKSGVAAGCRVPGKPNLLLGAYDIASLGYTADEFFVSGAACSYA
                     PDGEFGSDGRWDAVPSGVADYVTRIVVLRPTDDARFNGTVLVEWLNVSGGIDAPAVWF
                     LAHREVVREGYAYVAVSVQQVGVDGGASLLADMSLKTLDPERYSCLSHPGDAFSYDIF
                     SQVGQLVREGAGNGVLGPLSPEFVVAVGESQSAMFLTTYVNAVDPLVKVYDGFLVHSR
                     FGPAAPLDGMSIFAASESGVPEAVKFRPDLRVPLLTVITETDLVGAARAGYYLARQPD
                     NEWLRTWEIPGTAHADNYTIQVAPIDTGFAPLDQIVAAYAPTNTLMGQKLLYFINFAP
                     QHHYVLQAAIAGLHDWVRTGATAPDAPPVELSEGDPPQLILDANGIAKAGVRTPWVDV
                     PIARTSGIGVEGSVLSILFGTGEPFDAATLSRLYPGGAADYLERFTTSLDTAIRSGFL
                     LPADRLEILELAAATYPRGQ"
     gene            3371..>5004
                     /locus_tag="VF874_00695"
     CDS             3371..>5004
                     /locus_tag="VF874_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00374 family protein"
                     /protein_id="HEX9174656.1"
                     /translation="MRVDGRDITVSGSLLQPLTRRTNDILRLVLATVFLAIVIAGSLI
                     TRGRWVALEESVSEIVGVLPPTQANLIYLVYGVAIVALPFVILIGLIVGRRWKLLGAY
                     AAAGLIAVLALSITGNGIAAPRWHFDLSDRLDTFSSQFVDDPRWIAMLAAVLTVSGPW
                     LPARWRRWWWALLLAFVPIHLVVSAVVPARSLLGLAVGWFVGALVVLVVGTPALEVPL
                     HDAVRALGRRNFEVAALTVMHPAGRGPLVLSATAAQPDSAAIAEMYGPNQRSGGALRQ
                     LWRKFRLRGSETAPLQTSMRRAVEHRALMAIAIGDLGVANTSTIALAALDRGWTLYAH
                     TPARGNPIDEATPVGAVWESLRVLHDHQISHGDLRSKHITVDDGTVRFGGFGTAEYGA
                     TDAQLQSDIAQLLVTTTDVYDAPSAVSAAIAVFGNETILTASRRLTKSAVPKRIQESV
                     SDAGTVISAARDEVKHQTGADQIRTETITRFTRNQIIQLVLLVALVYVAYPFISTVPT
                     FFSELRTANWWWALLGLAVSALTYLGAAAALWACANG"
BASE COUNT          857 a         1676 c         1615 g          856 t
ORIGIN      
        1 gggtcctgcc acagcttgaa gaactcgttc gagatatcgt aatggaactg aacttcctcg
       61 gtatccgacc cgcgagtttg cgccgcggac ttcgacagcc acttggactg agcatgcgtc
      121 gaactctgcg tctcacttga agtacgtgcc atcgtccgtc ctgttgacac ttaggggatt
      181 ccgggacgtc ctgaatgccg ccggagggtc ctcccctact tcggattaca tcagactaca
      241 acgccggctg ctcgccgaga tgcaccccgc taccggcgtt gatggtcctc gccggtagcg
      301 gagctgcgcc gtggggtttt gcgcggtgcc gaggttttgg cggctgacat cacctcagcg
      361 aaggccgcgt ccatgcaatc cccgtagtgc tcgatatcgg gtagcacggc cgggcagccg
      421 ttgatgctga ccaccacacg cccgcagtaa ctgatcacgt tgtggatcag cccgcccccc
      481 tgtgtgaacg gcgaaaatgc gtagccgccg agcatccggg cgccgacgag gtagaccggg
      541 aagtcggggc cgcgcatcgt ggacaccagg gtgttggaga ttcgtgagtt ctcggcaagc
      601 cttcctaggc ctgccgccat cgcgagacgg agcgcgggaa cgaggacctc gcccggcagg
      661 aactcggcgt actcgagcac gcgcggggcg acctcctcgt tgaaggcctt cgaccgggtg
      721 ctggcgtcgt gcacgacccc caggcgggca acgggatctt cgaggtccgt gccgagtgga
      781 aggcgaacca cccccatctg attcactccg cggcggttgg ggtcggtgtg caccgcgatc
      841 gccacggccg tcacgagcga agtgtcgggc agctcgtcct tctcctggag ataccggcgc
      901 agcgcgcctc cgatgccggc gacgacgacg tcgttgacag tggcgtgcgg tacgagggtg
      961 cgtgccttca acaccgcgtc gagatcgtag gagcgtgcgt cgaagttgcg gctggtgtcg
     1021 atgtgcgcgt tgaaccggga agccggcacc tggtgggaag gccgctcatc ttcttgttct
     1081 tgacgaccga gcggcagccg ccgcgtcaac gctccgggaa ccttgctcaa caggggaagg
     1141 gtgtgtcgtg cagccgaggc aaactgcgcc gggcgacggg cgtaggtcac cgcggtgcgg
     1201 gccagcaggt tcagcgacga cggacgtggt tctggtgtcc agtcgtcgtc gggcggcggc
     1261 gcggtttcgg gggtcagatc gtgaagcgcg tcgaccaggg acaagcccgt caccccgtcg
     1321 accgccgaat gatggacctt gaagcacacg gcgaaggcgc ctttcggcac tcccttgatc
     1381 ctgccgagcc cctcgatgaa gtagccctcc cacgggggcc ggctcaaatc catccgctcg
     1441 gtgtgcagct gcgatatctg ctcgcacagc tgccgccagc caccgggtct cggcaatgtc
     1501 aggtggtgca ggtgccggct cagctcgaag ttctcgtcgc gcacccagta cgggtgatcc
     1561 agatcgaacg gaacgcgagc aagcaccgtg cgaaagatgc ggtccgcgcc gagccgggag
     1621 cggatatgcg cggcgacatc gtcgaaggtg acaacgccgc ccggagctgt cgacgggtca
     1681 tagatcgcga agtaggtcac gtgtgccgcg ccggcggtct cggcgtaaac gaatgccgca
     1741 tcaaggccac tcagctgctc cactacggcc tccggtgatc agcgctgatc tactcacctc
     1801 ctttgtactg ctgtgggcgt gtcggtgtat gcgattaacg acaccggcgc ttattgaccg
     1861 cgcgggtagg tggccgcggc gagctcgagg atttccagac ggtccgccgg taagaggaac
     1921 cccgaccgga tcgctgtgtc gagtgacgtc gtgaagcgtt cgaggtagtc cgccgccccg
     1981 cccggataga gccggctgag ggtggcggca tcgaacggct caccggtacc gaagagaatg
     2041 gacaggacac tcccctcgac gccgatgccg gatgtccggg caatcggcac gtcgacccac
     2101 ggagtacgga caccggcctt cgcgatcccg ttcgcatcca ggatcagctg aggtggatct
     2161 ccttcgctca actccacggg tggcgcgtcg ggcgcagtcg cgccggtgcg gacccagtcg
     2221 tgcaggccgg cgatcgccgc ttggaggaca tagtggtgct gcggggcgaa attgatgaaa
     2281 tacaacaact tctgacccat cagggtgttg gtcggagcgt aggcggccac gatctgatcc
     2341 agcggtgcga aaccggtgtc gatcggggca acctggatcg tgtagttgtc ggcatgagcg
     2401 gtccccggga tctcccaggt ccgcagccat tcgttgtcgg gctgcctcgc caggtaatag
     2461 cccgcccgcg cggcaccgac aagatcggtc tcggtgatga ccgttagcag agggacgcgc
     2521 agatcgggac ggaacttgac ggcttctggt acgccagatt ccgacgccgc gaagatggac
     2581 attccgtcca gcggtgccgc gggcccgaac cgggaatgga ccaagaagcc gtcgtacacc
     2641 ttcacgagcg gatcgacggc gttgacgtac gtcgtcaaga acatcgccga ttgagactca
     2701 cccacggcca caacgaattc cgggctcagc ggaccgagaa ctccgttgcc cgccccttcc
     2761 cggaccagtt ggccgacctg cgagaagatg tcatacgaga aggcgtctcc cgggtggctc
     2821 agacacgaat accgttctgg gtcaagtgtt ttcagcgaca tgtcggcgag gaggctcgca
     2881 ccgccgtcaa cgcccacttg ctgcaccgag acagcgacgt aggcgtagcc ctcgcgaacc
     2941 acctcgcgat gcgccaggaa ccagaccgca ggcgcgtcaa tgccgccgct gacatttagc
     3001 cactcgacga ggactgttcc gttgaacctg gcgtcatccg tcggcctcag caccacgatc
     3061 cgggtgacgt aatccgcgac acctgaaggc actgcgtccc agcgtccatc ggagccgaac
     3121 tccccgtcag gtgcgtagct gcacgccgcc cccgagacga agaactcatc ggcggtatag
     3181 cccaggcttg caatgtcgta agcgccgagc agcaagttcg gtttgcccgg aacacggcat
     3241 ccagccgcca cgcctgactt catcctgatg accctagcga agcgggttag tgcggaccga
     3301 aacccggtta ggttgtagat gctccgctga gggacgccgc cctgaagccg atccgggaga
     3361 tcgttgacgc atgcgagttg acggacggga catcaccgtg tccggcagct tgctgcagcc
     3421 gctgacccga cggaccaacg acatcctgcg gctcgtcctc gcgacggtat tcctcgcgat
     3481 cgtcatcgca ggctcgctga tcacgcgtgg gcgatgggtc gcgctcgagg aatccgtctc
     3541 cgaaatcgtc ggcgttctgc cacccaccca ggccaacctg atctacctcg tctacggcgt
     3601 cgcgatcgtg gcgttgccct tcgtcatcct gatcggcctc atcgtcggcc gtcgctggaa
     3661 actgctcggc gcgtacgcgg ccgccgggct gatcgccgtt ctcgcgctgt cgatcaccgg
     3721 caacggcatc gcggccccca ggtggcactt cgacctgtct gaccggctcg acacgttctc
     3781 gtcgcagttc gtcgacgacc cccgctggat cgcgatgctc gccgcggtgc tcacggtgtc
     3841 gggtccgtgg ctgcccgcgc ggtggcggcg ctggtggtgg gcgctgctgc tggccttcgt
     3901 gccgatccac ctcgtcgtca gcgccgtggt tccggcccgc tccttgctcg ggctggcggt
     3961 gggctggttc gtcggcgcgc tggtggtcct ggtcgtcggg acgccggcgt tggaggtgcc
     4021 gctccacgac gccgtccgcg cgctgggccg gcgcaatttc gaggtcgccg cgctcacggt
     4081 gatgcacccc gcagggcgcg gaccactcgt cctgtccgcg acggcggcgc aacccgattc
     4141 ggccgcgatc gccgagatgt acggccccaa ccaacgcagc ggcggtgcgc tgcgccaact
     4201 ctggcgaaag ttcagactgc gcggcagcga aaccgcgccc ctgcagacct cgatgcgccg
     4261 cgccgtcgaa catcgcgcgc tcatggccat cgccatcggc gacctcggcg tcgccaacac
     4321 ctcgacgatc gctctcgccg cgctcgaccg cggatggacg ctctatgcgc acacgcctgc
     4381 acgcggaaac cccatcgacg aagcgacacc cgttggcgcc gtgtgggaat cactgcgcgt
     4441 gttgcacgac catcagatct cccacggcga cctgcgcagc aagcacatca ccgtcgacga
     4501 cggcaccgtg cgcttcggcg gcttcggcac cgccgagtac ggcgccaccg acgcccagct
     4561 ccaatcagac atcgcccaac tcctggtgac gaccaccgac gtgtacgacg cgccgtcggc
     4621 cgtgagcgcg gcgatcgccg tcttcggcaa tgaaaccatt ctgaccgcat cccgccgact
     4681 cacgaaatcc gctgtgccga agcggattca ggagtcagtg agcgatgccg gcaccgtcat
     4741 ctccgccgcc cgcgacgagg tcaaacacca aaccggagcc gaccagatcc gcaccgagac
     4801 gatcacccgg ttcacccgca accagatcat ccaactcgtc ctgctcgtcg cgctggtcta
     4861 cgtcgcctac ccgttcatca gcaccgtgcc gacgttcttc tccgaactgc gcaccgcgaa
     4921 ctggtggtgg gcgctgctcg gcttagcggt gtcggcactc acctacctcg gcgcggcggc
     4981 cgcgctgtgg gcctgcgcca acgg
//
LOCUS       DASWBF010000025         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_790578,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000025 DASWBF010000000
VERSION     DASWBF010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790578"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..1933
                     /locus_tag="VF874_00700"
     CDS             422..1933
                     /locus_tag="VF874_00700"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9174657.1"
                     /translation="MAADSRPSPEELAAENAQLRALVGELMSHLEALEAENAELRRQL
                     GLNSQNSSKPPSSDSPFDKPAPKSLRRKSGRKPGGQPGHPGSTLALVDDPDETRRHEP
                     KVCAGCGAGLGGAVQLGVERRQVFDLPPMRVRVTEHQIVTRRCSCGAISCGAAPDGVR
                     APVQYGPRICAIILYLYVGQFLSKQRTAQALSELFGTPVSQGTVAAISERAADGLDEF
                     RTVVAVRIAAAEVAGFDETGLRVDGKLHWVHCARTDKYTLITCHPKRGKQGIDDAGVL
                     AGFSGVAVHDAWGPYDTYIDPEHQLCCAHALRELQGVADTAPPDAEWCWAVQARDALV
                     AMQQLITDAIAAGADTVDADALAEQVRLFRSAAQLGVTETAPRSSKTMKKHNALARRL
                     RDRQDDYLRFTTDRRIPPDNNGSERDIRMIKLRQKVSGCMRTLTGATQFCAIRSYLST
                     AAKHQRKRRLSSGLGNFSGSIVQAWSGWLVVGDSCQWARGRPLAGLAGQLPRG"
BASE COUNT          336 a          618 c          682 g          344 t
ORIGIN      
        1 gagtggcgaa gcttcgccgc acccgtcgcg gctacgagga agaccggccg cactactgtc
       61 cggccggtca tcaacttgga gcgaacaagg tgctcgtagg ggtcatgcac tgtgaatgcg
      121 gtgtgcttca ccggacccac tggtgcgcta cttgtggtgc gaccacgtac acaccgccgc
      181 tagggacgag ttgccgatca agagctctag acggtcgcgg ttgagcgatc gtttccaacg
      241 aggcgtattc actagacaat tggccttttt gaaattaatt ctttcgaaaa taattacggt
      301 atgaaagacg taactggtca ggtaatcgcg atgccgctgt tgctggtgtg atttttgggg
      361 tttctgtggc ttgatgggcg tgtcgcgttg atcttcgcgt ctggctgcga atcatggtgt
      421 agtggctgcc gattcacgcc cctcacctga ggagcttgct gcggagaacg cacagctgcg
      481 ggcgctggtg ggtgagctga tgtcgcacct ggaggcgctg gaggcggaga acgcggaact
      541 cagacggcag ttggggttga attcgcagaa ttcgtcgaag ccgccgtcgt cggattcgcc
      601 gttcgacaag cccgcgccga agtcgttgcg ccgcaagagc ggtcgcaagc cgggcgggca
      661 gcccgggcat ccgggttcga ccctggcgct ggtcgacgat ccggacgaga cgcgccgcca
      721 tgaaccgaag gtgtgtgccg ggtgcggcgc cgggctgggc ggcgccgtgc agctgggcgt
      781 ggagcggcgg caggtgttcg atctgccgcc gatgcgggtg cgggtgaccg agcaccagat
      841 cgtcacgcgc cgctgctcat gcggggcgat cagctgtggc gccgcgccgg acggggtgcg
      901 cgcgccggtg cagtacgggc cgcgaatctg cgcgattatc ctgtacctct acgtcgggca
      961 gttcctgtcc aagcagcgca ccgcacaggc actgtcggag ctgttcggta ccccggtgtc
     1021 gcaagggacg gtggcggcga tatccgaacg cgccgccgac gggctcgacg agttccgtac
     1081 cgtggtggcc gtgcgcatcg ccgccgcgga ggtggccggg ttcgacgaga ccgggctgcg
     1141 ggtggacggc aagctgcact gggtgcactg cgcgcgcacc gacaagtaca ccttgatcac
     1201 ctgccacccg aaacgcggca agcaggggat cgacgacgcc ggggtgctcg ccggcttcag
     1261 cggcgttgcg gtgcacgacg cctggggccc gtacgacacc tacatcgacc cggaacatca
     1321 gttgtgttgc gcgcacgcgt tgcgcgagtt gcagggtgtc gccgacaccg cgccaccgga
     1381 tgccgagtgg tgctgggccg tccaggctcg cgacgcgctg gtcgccatgc agcagctgat
     1441 caccgacgcc atcgcggccg gcgccgatac cgtcgacgcg gacgcactcg ccgagcaggt
     1501 gcggctgttc cgctccgccg cccagctcgg agtcaccgaa accgcaccgc gatccagcaa
     1561 aacgatgaag aagcacaacg cgctcgcccg caggctgcgt gaccggcaag acgactacct
     1621 ccgcttcacc accgaccggc gaatcccgcc cgacaacaac ggatccgaac gcgacatccg
     1681 catgatcaaa ctgcggcaga aggtctccgg ctgcatgcgc accctcaccg gcgcaacaca
     1741 gttctgcgcc atacgaagct acctatccac cgcagccaaa caccaacgta agaggcggtt
     1801 gtcaagtggt ctcgggaatt tttctggctc gattgtccaa gcctggtcgg gatggttggt
     1861 ggtcggcgat tcttgtcagt gggcgcgcgg gaggccgctg gccggcctgg ccggccagct
     1921 cccgcgtggt tgatgccctc atccgcggag tgctcggggg ctccgccgcg tgcgcctgcg
//
LOCUS       DASWBF010000026         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_856327,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000026 DASWBF010000000
VERSION     DASWBF010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856327"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1137
                     /locus_tag="VF874_00705"
     CDS             226..1137
                     /locus_tag="VF874_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9174658.1"
                     /translation="MARTDNDTWDLASSVGATATMVAAARAMASRSPEPLIDDPFAEP
                     LVRAVGIDFFTKVLDGDISLAQSDDPQFDPSRVIDLLAVRTKFFDEFFEHATATAGIR
                     QAVNLASGLDSRAYRLRWPEGTVVFEIDQQAVIDFKTGTLSALGAKPAAEHRPVAVDL
                     RDDWPAALRANGFDDTQPAAWSAEGLLAYLPPDAQDRLFDEITALSAADSRLATEYHP
                     QGSAFLAERNKLMARQWNDHELDLDLSKLMYHGERNPATDYLAACGWALTTQTRAELF
                     AAYGRQMPDDLASEPLRNTVAITAIRR"
     gene            1148..>1708
                     /locus_tag="VF874_00710"
     CDS             1148..>1708
                     /locus_tag="VF874_00710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9174659.1"
                     /translation="MARTDNDTWDVTSSVGATATMVATQRALATREGLIDDPFAEPLV
                     RAVDNDYFTRLVDGEIDLAELDPEFSLRRSAEGMAVRTRFFDRMFLDAGTTGVRQAVI
                     LAAGLDARAYRLPWPDGTVVYEVDQPEVLDFKTSTLAVLGAKPAAVHRPVAIDLRNDW
                     RNALLDNGFDPTAPTAWSAEGLLIYLP"
BASE COUNT          300 a          585 c          564 g          259 t
ORIGIN      
        1 cgcagccgag cgtctgcggc atgcaatggg gctggcgttg accagcgtcg ggttaggtcc
       61 tattccgtcc cgacccgccg caggcacagt catgtccggg gctgatgtca ggcgacaaac
      121 agtctacgag agtaacagtt agcggcggca gggcggcctg cgcaaccacg aagaatagcg
      181 gcgtacagtt acatcagcta attaacacct ctcaaggagc cagcggtggc acgcactgac
      241 aacgacacct gggatctggc gtccagcgtg ggagccaccg cgaccatggt cgccgctgcc
      301 agggcgatgg cgagcaggtc gcccgaaccg ctgatcgacg atccgttcgc cgagccgctg
      361 gttcgggcgg tcggcatcga cttcttcacc aaggtcctcg acggtgacat ctcgctggcc
      421 caatccgacg accctcagtt cgacccgtcg cgggtgatcg acctgctcgc cgtacgtacc
      481 aagttcttcg acgagttctt tgaacacgcg acggccaccg cgggaattcg gcaggccgtt
      541 aacctggcgt ccgggctgga ttcgcgcgcc tatcgactgc ggtggccgga gggcaccgtg
      601 gtgttcgaga tcgaccagca ggcggtaatc gacttcaaga ccggcacgct gtcggcactc
      661 ggcgccaaac ccgccgccga gcaccggccg gtggccgtgg atctgcgcga cgactggccg
      721 gcggcgttgc gcgccaacgg gtttgacgat acgcagcccg ccgcgtggag cgcggaaggc
      781 ctactcgcgt acctgccgcc cgacgcgcag gaccggctgt tcgacgagat cacggcactg
      841 agcgccgcgg acagccggct ggccaccgag tatcacccac aaggctcggc gttcctcgcc
      901 gagcgcaaca agctgatggc gcgccagtgg aacgaccacg agctggacct cgacctgtcg
      961 aagctgatgt accacggcga gcgcaacccg gccaccgact acctggccgc gtgcgggtgg
     1021 gcgctcacca cccagacacg agccgagctg ttcgccgcct acgggcggca gatgcccgac
     1081 gacctggcct ccgagccgtt acgaaacacc gttgccatca ccgcaatacg acgctgaagg
     1141 agcaccgatg gcccgaaccg acaacgacac gtgggacgtg acgtccagcg tgggcgccac
     1201 cgccacgatg gtggccacgc agcgtgcgct ggccacccgc gaggggctga tcgacgaccc
     1261 gttcgccgag ccgctggtgc gtgcggtcga caacgactac ttcacccggc tcgtcgacgg
     1321 cgagatcgat ttggcagagc tggacccgga attcagcctg cgccggtccg ccgagggcat
     1381 ggccgtgcgc acccgcttct tcgaccgcat gttcctcgac gccggcacga cgggtgttcg
     1441 tcaggcggtg atcctggccg cgggcctgga tgcacgggcg taccggttgc cgtggcccga
     1501 cggcacggtc gtgtacgagg tcgaccagcc ggaggtcctc gacttcaaga cgagcacgct
     1561 ggccgtgctc ggggccaagc ccgccgccgt ccaccgtccg gtcgcgatcg acctccgcaa
     1621 cgactggcgt aacgcgttgc tagacaatgg ttttgaccct acagcgccga ccgcgtggag
     1681 cgccgagggg ctgctgatct atctgccg
//
LOCUS       DASWBF010000027         9309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_790622,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000027 DASWBF010000000
VERSION     DASWBF010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790622"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF874_00715"
     CDS             <1..213
                     /locus_tag="VF874_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX9174660.1"
                     /translation="WVMGDNRTHSADSRAHCTSVPADLQRGVLCTGDPMSGTIPVENV
                     IGKARFIAWPPSRWGGVSSVDIQQGQ"
     gene            219..908
                     /locus_tag="VF874_00720"
     CDS             219..908
                     /locus_tag="VF874_00720"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9174661.1"
                     /translation="MQRAWPPRAVIRKSSGLRTLESALHNSGLGPVAGVDEVGRGACA
                     GPLVIAACVLGPNRLKSLADLDDSKKLTEKSREALFPLIRRYALAYHVVFVPPGEVDG
                     RGVHVANIEGMRRAVAGLAIRPGYVLSDGFRVPGLPMPSLPVIGGDAAAACIAAASVL
                     AKVSRDRLMVAMDAEYPGYGFAEHKGYCTSAHAAALAELGPSNVHRYSFINVRRSVVE
                     FVVPEERLEQA"
     gene            950..1255
                     /locus_tag="VF874_00725"
     CDS             950..1255
                     /locus_tag="VF874_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HEX9174662.1"
                     /translation="MSAEDLEKYETEMELSLYREYKDIVGQFSYVVETERRFYLANSV
                     ELIPRNTDGEVYFELRLADAWVWDMYRPARFVKQVRVITFKDVNIEEVEKPELRLPD"
     gene            complement(1266..1910)
                     /locus_tag="VF874_00730"
     CDS             complement(1266..1910)
                     /locus_tag="VF874_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HEX9174663.1"
                     /translation="MKGITRSLVALAAVSLALVTGVATTHAAMDNELSVVDGLGRTLN
                     VQQWDVLINGVTPLDNNPLSREWFHTGRAVYHVSGDGAEEFTGTLDFSYQVSFPWDMG
                     NGISFTYTTPNANLDQSNLPGILTGAPGSLLVTTPPLIPGVGISIDLGNGPGVQEVST
                     FSVPISGPDGAVAVAGAHGAVTGAAGGVTLRPYARLTSDDGDSVTTYGQPLEMH"
     gene            complement(2346..2765)
                     /locus_tag="VF874_00735"
     CDS             complement(2346..2765)
                     /locus_tag="VF874_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX9174664.1"
                     /translation="MADRPATMSDVHDIAAGMPHTTRIEGPKGNPVYQVGGKSFVFFR
                     NPRSDARDPVSGELYPDVIVIWVGSDADKTALVQDPDSPFFTTPHFAGHLSVLVRASH
                     LHRIARDELAELIQDAWLSRASKRRGATWLAEHGLDH"
     gene            3158..3526
                     /locus_tag="VF874_00740"
     CDS             3158..3526
                     /locus_tag="VF874_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX9174665.1"
                     /translation="MTMLTRAELGALGEQLAVDHLQSRGLKVLARNWRCRYGELDVIA
                     ADMPARTLVFVEVKTRTSDHFGGLAQAVNADKVRRLRRLAALWLAQQDARWAAIRIDV
                     IGVRIGRRRTPEIIHLEGVG"
     gene            3526..5038
                     /locus_tag="VF874_00745"
                     /pseudo
     CDS             3526..5038
                     /locus_tag="VF874_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            5035..6156
                     /gene="dprA"
                     /locus_tag="VF874_00750"
     CDS             5035..6156
                     /gene="dprA"
                     /locus_tag="VF874_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9174666.1"
                     /translation="MTEERRAWAYLSRVVEPPCAELSALAGRVGPMEAAERVRRGAVS
                     AQLAQLTEARRDIDCAADDLDRVARLGGRLLTPDDGEWPVLAFTSFNGVDVRRQPHGV
                     APLALWLLGGAPIDEVAWRAVAVVGTRAATDYGEYVAADLSEGLAKRGFAVVSGAAYG
                     IDGAAHRAALSADGCTVAVLAGGVDVPYPAGHSALLHRIQRTGLVVSEYPPGVRPARY
                     RFLVRNRLVAALSGASVVVEAGLRSGAANTAAWARGLGRVVCAVPGPVTSSASAGCHV
                     LIRQGAELVTRVEEIVEIVGRAGEFADEPEHPSSPLDGLTDIGKRVYEALPARGVRSS
                     DELSVESGLPVAQLQGQLARLELAGLAVQRDGRWRLAKS"
     gene            6306..7157
                     /locus_tag="VF874_00755"
     CDS             6306..7157
                     /locus_tag="VF874_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEX9174667.1"
                     /translation="MAGRPVHSFEVVRTERVSPHMVRVVLGGNGFDTFVPNQFTDAYC
                     KMLFLRDDVDVAALAVPLTLDSFQALPGDRQPVIRTLTVRSADPETREIAIDFVVHGE
                     HGVAGPWAPRVEPGRPVYLMGPSGAYAPDPSADWHLLAGDESALPAIAAALEALPADT
                     VARVFVEVAGPDDELELQSPADVDIYWIYRGGRADLVPEDRAGDHAPLIAAVKEARWL
                     PGRVQVFIHGEAQAVMHNLRPYIRKERGVDAKWASISGYWRRGRTEETFRQWKKELAA
                     AEAAEAG"
     gene            7237..8130
                     /locus_tag="VF874_00760"
     CDS             7237..8130
                     /locus_tag="VF874_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX9174668.1"
                     /translation="MLDEFADYLVLERGRSAHTRRAYLGDIGSLLDFLASSKQEPALA
                     DLTLPILRSWLAAQASTGAARTTLARRTSAAKTFTAWAARRGLLPSDPASRLQVPKAR
                     RTLPAVLRQDQALDAMAAAKSGAAENDPMALRDLLIVELLYATGIRVSELCGLDIDDV
                     DTGRRLLRVLGKGNKQRTVPFGEPALASLTRWLTEGRPALANGASGHALLLGARGRRL
                     DPRQARTVVHQTIAAVDGAPDIGPHGLRHSAATHLLEGGADLRVVQELLGHSTLATTQ
                     LYTHVSVARLRAVHDQAHPRA"
     gene            complement(8132..8620)
                     /locus_tag="VF874_00765"
     CDS             complement(8132..8620)
                     /locus_tag="VF874_00765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9174669.1"
                     /translation="MRRLALILAPLVVAPAAHADGYRFDWPLQPRPAIVRVFDAPTPN
                     WTRGHRGVDLAGAPGQQVYAAGPGAVVFAGELAGRPVVSIAHPGGLRTSYEPVRPAVR
                     AGQLVVQGAVIGELVAGHPDCPAAACLHWGAMWGPASAADYVDPLGLVETTPIRLKPV
                     GG"
     gene            8867..>9309
                     /gene="rpsB"
                     /locus_tag="VF874_00770"
     CDS             8867..>9309
                     /gene="rpsB"
                     /locus_tag="VF874_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9174670.1"
                     /translation="MAVVTMKQLLDSGTHFGHQTRRWNPKMKRFIFTDRNGIYIIDLQ
                     QTLTFIDKAYEFVKETVAHGGTILFVGTKKQAQESIAAEATRVGMPYVNQRWLGGMLT
                     NFSTVHKRLQRLKELEAMEQTGGFEGRTKKEILGLTREKNKLERSL"
BASE COUNT         1480 a         2994 c         3282 g         1553 t
ORIGIN      
        1 tgggtgatgg gcgacaaccg tacgcactcg gcggattcac gcgcgcactg caccagcgtc
       61 ccggccgatc tgcagcgcgg agtcctgtgc accggcgacc cgatgtcggg cacgattccg
      121 gtcgaaaacg tcatcggcaa ggcgagattc atcgcctggc caccgtctcg ctggggtggg
      181 gtcagttcgg tggacattca gcaagggcag tagtgcgcat gcagcgcgca tggccgccca
      241 gggcggtgat ccgcaagtcg tcgggcctgc gcaccctgga gtcggcgctg cacaacagcg
      301 ggctggggcc ggtggccggc gtcgacgagg tcggccgagg ggcgtgtgcc gggccgctgg
      361 tgatcgcggc ctgtgtgctc gggccgaacc ggctgaagag cctggctgat ctcgacgaca
      421 gcaagaagct caccgagaaa tcgcgggagg cgctgtttcc gctgatccgc cggtacgcgc
      481 tggcctacca cgtggtcttc gtgccgccgg gcgaggtcga cgggcgcggc gtgcacgtcg
      541 ccaacatcga gggaatgcgg cgcgcggtcg ccgggcttgc gattcgaccg gggtacgtgt
      601 tgtccgacgg cttccgcgtg cccggacttc cgatgccgtc gcttccggtg atcggcgggg
      661 acgccgcggc ggcgtgcatc gcggcggcca gcgtgttggc caaggtcagc cgcgatcggc
      721 tgatggtcgc gatggatgcc gagtatccgg gctacggttt cgccgagcac aagggctact
      781 gcacgtcagc ccacgctgcg gcacttgccg agctcggccc gtcgaacgtg catcggtact
      841 cgttcatcaa cgtgcgccgc tcggtggtcg agttcgtggt gccggaggag cggctggagc
      901 aggcgtgagg gaaaataggc atagacccga caaaaggaca gccgcgtaga tgagtgccga
      961 agatctcgag aagtacgaaa ccgagatgga gctctcgctc taccgcgaat acaaggacat
     1021 cgtcggacag ttcagctacg tggtggagac cgagcgccgg ttctatctgg ccaatagcgt
     1081 cgagctgatt ccccgcaaca ccgacggtga ggtgtacttc gagctgcgcc ttgccgacgc
     1141 ctgggtgtgg gacatgtacc ggccggccag attcgtcaag caggtgcggg tgatcacgtt
     1201 caaggacgtc aacatcgaag aggtggagaa gcccgagctg cggctgcccg actgagtccg
     1261 agtgttcagt gcatctccag cggctggccg tacgtcgtga cgctgtcgcc gtcgtcggac
     1321 gtcagcctcg cgtacgggcg cagcgtcacg ccaccggcgg cgccggtcac cgcgccgtga
     1381 gctcccgcga ctgcgacggc gccgtcgggc ccggagatcg gaacagagaa ggtcgaaacc
     1441 tcttgtactc ctgggccgtt cccgaggtcg atactgatgc ccacgcccgg aatgagcggc
     1501 ggtgtcgtga cgagcagcga acccggcgcc ccagtgagaa tgcccgggag atttgactga
     1561 tcgaggttgg cgttgggcgt cgtataggtg aagctgatgc cgttccccat gtcccacggg
     1621 aagctgacct gataactaaa atctagtgtg ccggtgaact cttcggcccc gtcaccgctg
     1681 acgtgataca ccgcgcgccc ggtgtggaac cactcgcgac tcagcgggtt gttgtccaac
     1741 ggggtcacac cgttgatcag cacgtcccac tgctggacat tgagcgtccg gcccagtccg
     1801 tccacgacgc tgagctcgtt gtccatcgcg gcatgtgttg tcgcgacacc ggtgactagc
     1861 gcgaggctta cggcggccag cgcgaccaac gaccgggtga tccccttcat cttctcctcg
     1921 gctgcaactg ggtggccgca actagtcggc cggtcaagaa gtcaaggtgt tgatttccct
     1981 gtgacgcaaa aggattggct gttccgttgg catcgatgaa gaagataacc gcgccagatc
     2041 cgcctgagca atagtcgctg ttatttgctg gccgaactga cacagagttg ttgccattcg
     2101 gcattcgcgt cgtaatcgcc attcgcgcag tgttggcttt cgatgtggta tgcccgtatt
     2161 cacgggcgaa tccgtcggtg agccgcgaaa atcgctgttc tgacgtcggt ttgggctaca
     2221 gcttagacgg atcgcttact tgtcacgcgc gcaaacgggt ggggtcggtg taaccgccag
     2281 cacggcaacg cgagcagcgt gagcagcgcc ccgatcgagt gagacgcttg cctgcgtcgc
     2341 cacggtcaat gatcgaggcc atgctcggcc aaccacgtgg ccccccgtcg cttcgaggct
     2401 cgcgacagcc acgcatcttg aatcagttcg gcgagttcgt ctcgcgcgat gcggtgcaaa
     2461 tgcgatgcgc gcacaaggac cgacaggtga ccggcgaagt gcggggtggt gaagaagggg
     2521 ctgtcagggt cctggacgag ggccgtcttg tcggcgtcgg atccaaccca gatcacgatg
     2581 acgtctgggt acagttcgcc gctgaccgga tcccgcgcgt ccgaacgcgg gttgcggaag
     2641 aacacgaacg atttcccgcc gacctggtag acggggttgc ctttcgggcc ttcgatgcgc
     2701 gtggtgtgcg gcatgcccgc cgcgatgtca tggacgtcgc tcatcgtggc cggtcgatca
     2761 gccatccaaa aagactacgg cgctatgact tcggtgcaac gccaaggact tgcaatgatg
     2821 gcgctcattt cgctggttta ggcccctgcc gtctgcgtgc gttcaaatca gcggtcgggg
     2881 aaagtgaccg caggcgaacc tgcgggcgta caacaacgat ctgcggggaa tcgcacagcc
     2941 gctggtcgat ctacgaacgt cgcgcaatca gaacgtgtct gcggcgcaga tcgcggcgat
     3001 tctgcaggcg ttcacctcct cgtagcgtcg gcggtttcgt ttcgcgcctg tggacgaaac
     3061 gcaggttggg gataaccagc cctgatcggt gaggtgtggc cgcacgtcgg ccgcaccttg
     3121 tccgctggtt gtcgggggcg cgctccacgc tgagcgcatg acaatgttga cccgcgccga
     3181 actcggcgca ctgggtgagc aactcgcagt cgaccacttg caaagccgtg gcctcaaggt
     3241 gctggcccgt aactggcggt gccgttacgg cgagctcgat gtgatcgccg ccgacatgcc
     3301 cgcgcgcacc ctggtgttcg tcgaggtgaa gactcggacc tccgaccatt tcggcggtct
     3361 ggcgcaggcg gtgaacgccg acaaggtgcg tcggctgcgc cggctggccg cgctgtggct
     3421 ggctcaacag gacgcccgct gggcggcgat ccgcatcgac gtgatcggtg tgcgaatcgg
     3481 ccgtcggcgc acgcccgaga tcattcatct ggaaggtgtc ggctgatggc cctcggccgg
     3541 gcgttctcgg tcgccgttcg gggcgttgcc ggagagatcg tcgaaatcga ggcggacatc
     3601 acctccggtc tgccgggcgt tcacttggtc ggcctgccgg atgctgcgct gtccgaatcg
     3661 cgggaccgtg ttcgcgcggc gatcatcaac tgcggtaatc gctggccgat gtcgcggctg
     3721 acgctggcat tgtcgccggc gacgttgccg aagatgggtt ccgtctacga cgtcgctttg
     3781 gctgccgcag tgctgtcggc ggaaccgctc ggtcgctgcc gcgcggttgg agaagacggt
     3841 tctgctgggt gagctggcgc tggacggaag agttcgtccg gttcgcggcg tgctgccggc
     3901 cgtgatcgcc gcgaaactgg agggctggcc ggctgtggtg gttccagtcg agaaccacgc
     3961 cgaggccgcg ctggtggacg gcatcgaggt gcacggagcc cgcacgcttc ggcagctgca
     4021 gtcctggctc aacggcgcgg gtgagctcga tcagacgtgc cggatggagg ccgcggcacc
     4081 ggacgacggg cccgacctcg ccgatgttgt cgggcagccc caggcacgct tcgccgtcga
     4141 gatggccgcc gcgggcggtc atcatcttat gctcgtcggg ccgccgggca tcggcaaaac
     4201 gatgctggcg caacgacttc cgggcttact cccaccgctc accgaggagg agtcgctgga
     4261 ggtgacggcc attcactccg tcgcagggct gctgtcggcc gagaccccgt tgatctcgcg
     4321 accgccgctg gtcgctccgc accacacgac ttccgtggcg gcgctgatcg gaggagggag
     4381 cggacttgcc cggcccggtg cggtcagccg cgctcaccgc ggcgtgctgt ttctcgacga
     4441 atgccccgag atcgggctgc atgcgttgga agcgctgcga actccgttgg aggaaggcca
     4501 agttcggttg gcacggcgcg acggggtggc cgtctacccg gcccgctttc agttggtgct
     4561 ggccgcgaac ccgtgcccgt gcgcgccggc cgacccgcgc gactgcatct gcgcgggggc
     4621 ggccaagcgc cgctatctcg gcaggctgtc cggtccgctg ctcgatcggg tcgacctgcg
     4681 ggtgcagatg caccgggccc ggaccggagc actcaacgcc gacgtcggcg aacccacgcg
     4741 cgtggtacgc gagcgggtcg tggcggcacg ggcggccgct gcggcgcgat ggcgccccta
     4801 cggtgtgcgg accaacgccg aagtgtcggg tccgcttctg cggcaacggt ttcggctgga
     4861 caacgtcgcc atggaaccgc tgcggcaggc actcgataaa gggctgctga gcatcagggg
     4921 agtggaccgc acgatccgcg tcgcatggac actcgccgat ctcgccggcc gggagcggcc
     4981 cggcatcgag gaagtcacca cggcgatggg atttcgtcag acgggagccg accgatgacc
     5041 gaggagcgac gagcatgggc ttacctgtcg cgggtggtgg agccgccgtg cgccgaactg
     5101 tcggcgctcg ccggccgtgt cggcccgatg gaggcggcgg agcgcgtgcg acgaggtgcg
     5161 gtttccgccc aacttgccca gctcactgag gcgcgccgcg atatcgactg cgccgcagac
     5221 gatctcgacc gcgtcgctcg gctgggcggg cggcttctca cgccggacga cggggagtgg
     5281 ccagtgctgg cgttcacgtc gttcaacggc gtcgacgtgc gtcggcagcc gcacggcgtc
     5341 gcgccgctgg cgctgtggct gctcggtggt gcgcccatcg acgaggtggc ttggcgggca
     5401 gttgcggtcg tgggcacgcg agcggcgacc gactatggcg aatacgtcgc ggccgatctg
     5461 tcggaggggc tagccaaacg cggctttgca gtggtgtcag gcgccgcgta cgggatcgac
     5521 ggcgctgcac accgggcggc cttgtcggcg gacggctgca cggtcgcggt cctggcgggc
     5581 ggtgtcgacg ttccgtatcc ggccgggcat tcggcgctgt tgcaccgcat ccaacgcacc
     5641 ggcctcgtgg tgagcgagta cccgcccggc gtgcgtcccg cgcggtaccg cttcctggtc
     5701 cgcaatcgat tggtcgccgc gttgtcgggg gccagtgtcg tggtggaggc ggggctgcgc
     5761 agcggagcgg ccaacaccgc ggcgtgggcg cgcgggctgg gccgagtggt gtgcgccgtg
     5821 ccgggcccgg tgacgtcatc ggcgtcggcc gggtgccatg tcttgatccg gcagggcgcc
     5881 gagctggtca cccgggtcga ggagatcgtc gagatcgtcg ggagagcggg cgagtttgcc
     5941 gacgagccgg aacacccgtc gtcgccgttg gacgggctga ccgacatcgg aaaacgtgtg
     6001 tacgaagcgc tgcccgcccg cggcgtgcgg tcaagcgacg agttgtcggt cgaatccgga
     6061 ctacctgtcg cgcaattgca gggccaactc gcgcggctcg aacttgccgg cctcgcagtt
     6121 cagcgcgacg gtcgctggcg attggcgaag tcataaattc ccggccggtg cgttcaccgc
     6181 gctggtcggc atcgtcgttc tctgcctggg ggcgctcgca gcgatcggtg cgatgttgta
     6241 ggcaggccac cagcggcgcc cggcccgtat agtccagatc agctcatcaa ccgtcgggag
     6301 gcgatgtggc gggtcggccg gtgcacagct tcgaggtcgt gcggaccgaa cgcgtgtcgc
     6361 cgcacatggt gcgagttgtg ctcggcggca acggcttcga cacgttcgtg ccgaatcagt
     6421 tcacggatgc ctactgcaag atgttgttcc ttcgcgacga cgtcgacgtc gcggctcttg
     6481 ccgtgccgct cacactggac agctttcagg cgttgcccgg cgaccgccag cccgtgatcc
     6541 gcaccctgac cgtccggtcg gccgatcccg agacacgtga gatcgcgatc gacttcgtcg
     6601 tgcacggcga gcacggcgtg gcgggaccct gggcgccgag ggtcgaaccg ggccggcccg
     6661 tttacctgat gggtccgagt ggggcctacg cacccgatcc cagcgccgac tggcatctgc
     6721 tggccggaga cgaatccgct ctgcctgcca tcgccgccgc gctcgaggcg ctccccgctg
     6781 acaccgtggc ccgggtgttc gtcgaagtag cgggcccgga cgacgaactt gagctgcagt
     6841 ctccggcaga cgtggacatc tattggatat accgcggcgg gcgtgccgac ctggttcccg
     6901 aagaccgcgc gggcgaccac gcgccgctca tcgccgcggt gaaggaagcc aggtggctgc
     6961 ccgggcgggt gcaggtgttc attcacggtg aggcgcaggc cgtcatgcac aatctgcggc
     7021 cgtatatccg caaggaacgc ggggttgacg ccaaatgggc gtcgatatcg ggctactggc
     7081 gtcgcgggcg gacggaagag acgttccggc agtggaagaa agagctggct gcagctgaag
     7141 cggccgaagc cgggtagaca cgcggcgcgc ttggcgggct cggcggcgct gatctgcgac
     7201 cgtggtcacg tgacccgatc ggccgcgttc gccccgattc tcgacgagtt cgccgactat
     7261 ctggtgcttg aacgcgggcg ttccgcacac acccggcggg catatctcgg cgacatcggg
     7321 tcgctgctcg acttccttgc ttcgagtaag caggagcctg cgctggctga cctgacgctg
     7381 ccgatactgc gttcgtggct ggccgcacaa gcgtcgaccg gtgccgcccg caccacgctt
     7441 gcccgtcgca cctcggcggc caagacgttc acggcatggg cggcgcgacg cggcctgctg
     7501 ccgtcggatc cggcgtcgcg tctgcaggtg cccaaggcgc ggcgcacgct gcccgccgtg
     7561 ctgcgacaag accaggcact tgacgcgatg gcggccgcca aatccggtgc ggccgaaaac
     7621 gatccgatgg cgctgcgcga tctgctgatc gtcgagctgc tctatgcgac cggcatccgc
     7681 gtcagcgagc tgtgcggcct ggacatcgac gacgtcgaca ccggccggcg attgctgcgc
     7741 gttctgggga agggcaacaa acagcgcacc gtgccgttcg gcgagccggc actggcgtcg
     7801 ctgacccgat ggttgaccga gggtcggccg gcgctggcca acggggcctc gggccacgcg
     7861 ctgctgcttg gcgcccgggg tcgccggctt gacccacgcc aggcgcgcac cgtcgtccat
     7921 cagacgatcg ccgccgtcga cggcgccccc gacatcgggc cgcacgggtt acgccacagc
     7981 gccgcgacgc acttgctcga aggcggagcc gaccttcgcg tggtgcagga actgcttggc
     8041 cactcgacgc tggcgacgac gcagctgtat acgcatgtgt cggtggctcg gctgcgggcg
     8101 gtgcacgacc aggcgcaccc gcgggcctga gtcacccgcc cacaggcttg agccggatcg
     8161 gcgtcgtctc gacgagcccc agcgggtcga cgtaatcagc cgccgacgcc ggaccccaca
     8221 tcgctcccca atgcaggcat gccgccgccg gacagtcagg atggcccgcc accaattcac
     8281 cgatcacggc gccttgaacg accagctgac ccgctcgcac cgccggccgc accggttcat
     8341 aactggtccg tagcccgccc ggatgcgcga tcgacaccac tggccgcccg gccagctcac
     8401 cggcgaacac cacagccccc ggcccggcgg cgtacacctg ctgacctggg gcaccggcca
     8461 ggtcgacgcc gcggtggccg cgagtccagt tcggcgtcgg cgcgtcgaac acgcgcacaa
     8521 tcgccgggcg gggctgcagc ggccagtcga accggtaacc gtcggcgtgg gcggccggag
     8581 cgaccactag cggggcgaga atcagggcga gccgtcgcat gcgtccagtc gagcgcagct
     8641 tccggggcgt ggaccagggc tgtcggctca gctgtggaca aagcagctgt tggggacgtg
     8701 taaactccta cccgcagctt gcatcagcga gctgacttcg cgcgcctgca gccaaccgaa
     8761 cgccgtgcgg tcccgtcgtc acgacgggtg cggtgttccg caggtgccag ggcccgtcat
     8821 cacccgatgc cgggcggcaa ccgacaacat aaggacatgg ccgactatgg ctgttgtaac
     8881 catgaaacag ctgctcgaca gcggcaccca cttcgggcac cagacccgtc gctggaatcc
     8941 caagatgaag cggttcatct tcaccgaccg caatgggatc tacatcatcg acctgcagca
     9001 gacgctgacc tttatcgaca aggcgtacga gttcgtcaag gagaccgtcg cccatggcgg
     9061 gacgatcctg ttcgtcggca ccaagaagca ggcgcaggag tccatcgcgg ccgaggccac
     9121 ccgggtcggc atgccgtatg tgaaccagcg ctggctgggc ggcatgctca ccaacttctc
     9181 gaccgtgcac aagcggctgc agcgcctcaa ggaactcgag gcgatggagc agaccggcgg
     9241 cttcgagggc cgcaccaaga aggagatcct gggcctgacc cgcgagaaga acaagctgga
     9301 gcgcagcct
//
LOCUS       DASWBF010000028         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1119479,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000028 DASWBF010000000
VERSION     DASWBF010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119479"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VF874_00775"
     CDS             complement(<1..852)
                     /locus_tag="VF874_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX9174671.1"
                     /translation="MSVPAIVIAAPASGSGKTTVATGLIGALHRIGHTVAPFKVGPDY
                     IDPGYHTLAAARPGRNLDPVLVGERLIGPLYRHGTAGADIAVVEGVMGLFDGRIDSAA
                     DMARGSTAHVAGLLGAPVVLVVDARGQSQSIAALLHGFSTFDASIRIAGVVLNRVGSA
                     RHEEVLRRSCAQSGVTVLGAIPRHVELELPSRHLGLVTAAEHGTKAHRAVDAMTELVA
                     EHVDLAGVTAVAASNVMARAWEPTLDSATAPVTVAIAGGKAFTFGYAEHVELLQAAGA
                     DVVAFDPL"
     gene            complement(941..1555)
                     /gene="cobO"
                     /locus_tag="VF874_00780"
     CDS             complement(941..1555)
                     /gene="cobO"
                     /locus_tag="VF874_00780"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX9174672.1"
                     /translation="MPQGRPIAIPDDGLTTRARRQAALLAVHTGTGKGKSTAAFGMAL
                     RAWSAGLSVAVFQFVKSAKWKVGEETAFRELGRAHDERGVGGPVEWHKMGAGWSWTRK
                     AGTVDDHAAAAADGWAEIARRLAQQRHDFYLLDEFTYPLKWGWVDVDEVVSALAGRPG
                     TQHVVITGRDAPPQLLDAADLVTEMTKVKHPMDAGRKGQIGIEW"
     gene            complement(1570..3372)
                     /locus_tag="VF874_00785"
     CDS             complement(1570..3372)
                     /locus_tag="VF874_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9174673.1"
                     /translation="MTPVYPFSAIVGQELLRLALVLCAVRPEIGGVLIRGEKGTAKST
                     AVRGLASVLAEAGFGRLVELPIGATEDRVIGSLDLQRVLRDGEHAFTPGLLAEAHGGV
                     LYVDEVNLLHDHLVDVLLDAAAMGRVHIERDGISHSHEAKFILVGTMNPEEGELRPQL
                     LDRFGLTVDVRASRDVDERADVIRQRMSFEADPSAFARRYADQDAALAQRIATARLAV
                     GDVVLPDRELRRIASLCAAFGVDGMRADLVVARAAVAHAAWRGADVVDEVDIRVAAQL
                     ALPHRRRRDPFDEAELDPQMLDDALAQAGHDDLDPEPDPPGGGMSNVEEAQDHSGPSP
                     TRPSAPPAAPFRTRLLTVPGVGDGAPGRRSRARNRSGAVVSAHATDGFGVHLFGTVLA
                     AARRAPTPGPPQVESDDLRRAIREGREANLVIFVVDASGSMAARDRMAAVSGATLSLL
                     RDAYQRRDKVAVITFRGADARVLLPPTSSVHVASRRLTRFDTGGKTPLAEGLLAARGM
                     VARERVRDPARRSLVVVLTDGRATGGSDPVGRAGRAADLLVAEGGSAVVVDCETSWLR
                     LGLAEQLAGRLGAPTVRLAQLRADGLTELVRGAA"
     gene            complement(3369..3836)
                     /locus_tag="VF874_00790"
     CDS             complement(3369..3836)
                     /locus_tag="VF874_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9174674.1"
                     /translation="MTVTLHRAWAKDLDTATLYELLKLRVEVFVVEQACAYPELDGRD
                     LLPETRHFWLEGPRGEVICTLRLMEEHAGGQKAFRLGRLCTRREARGHGHTTRLLQAA
                     LADVGDYPCRIDAQTYLEEMYAQHGFARAGEEFVEDGIPHVPMVRSVMRGGAP"
     gene            complement(3833..>4030)
                     /locus_tag="VF874_00795"
     CDS             complement(3833..>4030)
                     /locus_tag="VF874_00795"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEX9174675.1"
                     /translation="LLGASPGASTAVPAMLDVLERCFPDRYQLWLPKLKEMVPSLGTK
                     LSEEPRLFEEVWDWTTKALGA"
BASE COUNT          631 a         1519 c         1306 g          574 t
ORIGIN      
        1 gagcgggtcg aacgccacga cgtccgcacc ggccgcctgc agcagctcga cgtgttcggc
       61 ataaccgaat gtgaacgcct tgcctcccgc gatcgcgaca gtcaccggcg ctgtcgcgct
      121 gtcgagcgtc ggctcccacg cgcgcgccat cacattgctc gcagcgaccg ctgtcacgcc
      181 ggccaggtcg acgtgctcgg cgacgagctc ggtcatcgcg tcaaccgccc ggtgtgcttt
      241 cgtgccgtgt tctgccgcgg tgacgaggcc cagatgcctt gagggtagtt ctaattcgac
      301 gtgccgaggg atcgcgccga gcaccgtcac acccgactgt gcacaggacc gccggagcac
      361 ctcctcgtga cgggcggagc cgacacggtt gagaaccacc ccggcgatcc ggatcgatgc
      421 gtcgaacgtc gaaaacccgt gcagcagtgc cgcaatgctc tggctctggc cgcgggcgtc
      481 gacgaccagg accaccggag ccccgagcag gccggcgacg tgagcggtcg aaccccgcgc
      541 catgtctgca gcgctgtcga tgcgcccgtc gaacaggccc atcacgcctt ccaccaccgc
      601 gatgtcggcg cccgcggtcc cgtgccggta cagcgggccg atgaggcgct cgccgaccag
      661 caccgggtcg aggttgcggc cggggcgggc agcggccagc gtgtggtagc cggggtcgat
      721 gtagtcgggg ccgaccttga acggcgcgac ggtgtgcccg atgcggtgca gcgcaccaat
      781 caaacctgtt gccaccgtgg tctttccact ccccgaagca ggtgcggcga tgacgatagc
      841 cgggacgctc accgccccct ccctcgccgc gagcgtgcgg tgttgtacgt atttccccgg
      901 cgtgtcgcgt acagacacgc acgctcgcgg caatcgaggg tcaccattcg atgccaatct
      961 ggcccttgcg gcccgcgtcc atcgggtgct tcaccttggt catctcggtg acgaggtcgg
     1021 cggcgtcgag cagctgcggc ggcgcgtcgc ggccggtgat caccacatgc tgcgtgccgg
     1081 gccggccggc aagcgcggac accacctcgt cgacgtccac ccagccccac ttgagcggat
     1141 aggtgaactc gtcgagcaga tagaagtcgt ggcgctgctg cgccagtcgc cgcgcgatct
     1201 ccgcccagcc gtcggcggcc gcggcggcgt gatcgtcgac cgtgccggcc ttgcgggtcc
     1261 acgaccagcc ggcacccatc ttgtgccact cgacaggtcc gccaacccca cgctcgtcgt
     1321 gtgcacggcc caactcacga aaagcggtct cctcgcccac tttccacttc gcgctcttga
     1381 cgaactggaa caccgcaacc gacaagcccg cgctccacgc ccgcagcgcc atcccgaacg
     1441 cggccgtcga cttgcccttg cccgtcccgg tgtgcactgc gagcagcgcg gcctgccggc
     1501 gcgcccgggt ggtcagcccg tcgtcgggta tcgcgatcgg ccggccctgc ggcatagtga
     1561 ctctccttct caggcggcgc cgcggacgag ctcggtaagg ccgtcggccc gcagctgcgc
     1621 caaccgcacc gtcggcgcac cgagccggcc tgccagctgc tccgccaatc ccagtcgtag
     1681 ccaggaagtt tcgcagtcga cgaccacggc ggacccgcct tcagcgacga gcagatccgc
     1741 tgcgcgaccg gccctgccca ccggatcgga accgccggtc gcgcgaccgt cggtgagcac
     1801 gacgaccaag ctgcgccggg ccggatcgcg tacgcgttcc cgagcgacca tgccgcgggc
     1861 ggccagaagc ccctccgcca gaggcgtttt gccgcccgtg tcgaaacgcg tcagccggcg
     1921 cgacgcgacg tgcaccgacg acgtcggcgg aagcagcacc cgagcatccg cgccgcggaa
     1981 cgtgatcacc gcgaccttgt cgcgacgctg gtacgcgtcg cgcaacagcg acagcgtcgc
     2041 cccgctcacc gcggccatcc ggtcgcgtgc ggccatcgac ccggatgcgt cgacgacaaa
     2101 aatcaccaga ttcgcctcac gcccttcgcg gatcgcccga cgcaggtcgt cggactccac
     2161 ctgaggcgga cctggcgtag gtgcgcgccg cgccgcggcc agtaccgtgc cgaacagatg
     2221 aaccccgaac ccgtcggtgg catgcgcgga caccacagca ccggaccggt tgcgggcccg
     2281 cgaacggcgg cccggcgcac cgtcgccgac acccggcacc gtgagcagtc gggtgcgaaa
     2341 cggcgcggcc ggtggcgcgc tgggccgagt cggcgatgga cctgaatgat cttgggcttc
     2401 ttcgacattc gacataccgc cgcccggtgg atcgggctcg gggtccagat cgtcgtggcc
     2461 ggcctgcgcc agcgcatcgt cgagcatctg cgggtcgagt tccgcttcgt cgaacggatc
     2521 gcgacgacgg cggtgcggca gcgccagctg cgccgccacc ctgatgtcca cttcgtcgac
     2581 gacgtccgcg ccgcgccagg ccgcgtgcgc caccgcggcg cgcgccacca ccaggtcggc
     2641 ccgcattccg tcgacaccga acgccgcgca cagcgacgcg atccgccgca gctcgcggtc
     2701 gggcagcacc acgtcaccga ccgccaatcg cgcggtggcg atccgctgcg ccaacgcggc
     2761 gtcctgatcg gcgtagcggc gggcaaacgc ggaggggtcg gcctcgaacg acatccgctg
     2821 tcggatcacg tccgcgcgct cgtcgacgtc acgcgaggcc cgcacgtcga ccgtcagccc
     2881 gaacctgtcg agcagctgcg gccgcagttc cccctcctcc gggttcatcg tgcccaccag
     2941 gatgaacttg gcctcgtggc tgtgggaaat gccgtcgcgt tcgatgtgca cccggcccat
     3001 cgcggcggcg tcgagcagca cgtccaccag atggtcgtgc agcaggttca cttcgtcgac
     3061 gtacagcacg ccgccatgcg cctcggccaa aagcccaggc gtgaaagcgt gttcgccgtc
     3121 gcgcagcacc cgctgcagat ccagcgaccc gatcacccgg tcctcggtgg caccgatcgg
     3181 cagctccacc agccgcccaa agcctgcttc agccaagacg gacgccagcc cccgcaccgc
     3241 cgtcgacttc gcggtgccct tctcaccgcg gatcagcacg ccgccgatct ccgggcgcac
     3301 ggcgcacagc accaacgcca gccggagaag ctcctgcccg acgatcgcgc tgaacgggta
     3361 cacgggtgtc atggcgcgcc cccgcgcatg acggaccgca ccatcggcac gtgcgggatg
     3421 ccgtcctcga cgaattcctc tccggcacgg gcaaatccgt gctgtgcgta catctcctca
     3481 aggtaggtct gcgcgtcgat ccggcacggg tagtccccca cgtccgccaa cgccgcctgc
     3541 agcaggcgcg tagtgtggcc gtgcccgcgg gcttcgcggc gggtgcacag ccggcccagg
     3601 cggaacgcct tctgcccgcc ggcgtgctcc tccatcagcc gcagggtgca gatcacctca
     3661 ccccgcggac cctcgagcca gaagtgacgg gtttccggca gcaggtcccg gccgtccagt
     3721 tccgggtacg cgcaggcctg ttcgacgacg aacacctcga cccgcagctt gaggagttcg
     3781 taaagagtcg cggtgtcgag atccttggcc catgcccggt gcaacgtgac cgtcatgcgc
     3841 ccagcgcctt cgtagtccag tcccacactt cctcgaacag ccgcggctcc tcggacagct
     3901 tcgtgccgag cgacggcacc atctccttga gcttcggcag ccacaactgg tagcggtcgg
     3961 gaaagcaccg ctcgagtacg tcgagcatgg ccggaaccgc cgtcgaggcg cccggagacg
     4021 caccgagcag
//
LOCUS       DASWBF010000029         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_988017,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000029 DASWBF010000000
VERSION     DASWBF010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988017"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..77
                     /locus_tag="VF874_00800"
     CDS             <1..77
                     /locus_tag="VF874_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9174676.1"
                     /translation="FGENFRWVVLADPEGNAFCVAGSS"
     gene            291..>2117
                     /gene="purL"
                     /locus_tag="VF874_00805"
     CDS             291..>2117
                     /gene="purL"
                     /locus_tag="VF874_00805"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX9174677.1"
                     /translation="MGLKDDEYARIREILGRRPSDAELAMYSVMWSEHCSYKSSKVHL
                     RYFGETTTDEMRKAMLAGIGENAGVVDIGDGWAVTFKVESHNHPSYVEPYQGAATGVG
                     GIVRDIMAMGARPVAVMDQLRFGAADAADTRRVVDGVVRGIGGYGNSLGLPNIGGETV
                     FDASYAGNPLVNALCVGALRKEDLHLAFASGTGNKIILFGARTGLDGIGGVSVLASET
                     FGGDESGSGRKKLPSVQVGDPFMEKVLIECCLELYAADLVVGIQDLGGAGLSCATSEL
                     ASAGGQGMAVELDNVPLRTANMTPAEVLASESQERMCAVVKPENVDAFLAVCRKWEVL
                     ATVIGEVTDGDRLRITWHGEAVVDVPPRTVAHEGPVYQRPVKRPDSQDALNADTSARL
                     PRPTTGDELRATLLALLGSPHLCSRAFITEQYDRYVRGNTVLAEHADGGVLRIDERTG
                     RGIAVSTDANGRYTQLDPYTGAQLALAEAYRNVAVTGATPVAVTNCLNFGSPEDPGVM
                     WQFSQAVRGLADGCAELGIPVTGGNVSFYNQTGSTAILPTPVVGVLGVLEDVSRRIPT
                     AFGTEPSKTLMLLGDTRDEFDGSIWAQVTADHLGGRPPAVDLQ"
BASE COUNT          340 a          695 c          742 g          340 t
ORIGIN      
        1 cgttcggcga gaacttccgt tgggtggtgc tcgccgatcc cgagggcaac gcgttctgtg
       61 tggccggcag tagctaagcg cgacactgcg gcgccaagtg agccccggcg cgtggcggac
      121 gggagcgcgg cgggtcggga acttaagcta tctgggtgac gtcaccgctc gccgagtctg
      181 attctcttcg cgcaagcggc tcatcgccga cgccgccgca gaccacgccc tcgctcgata
      241 ccgtcgagca cgcggcggcg acgccggacc ttccgcagcc gtaccgtgag ctgggcctca
      301 aggacgacga gtacgcgcga atccgcgaga tcctcggccg ccgtccttcc gacgccgagc
      361 tggccatgta ctcggtgatg tggagcgagc actgctccta caagtcgtcg aaggtgcatc
      421 tgcgctactt cggcgagacc accaccgacg agatgcgaaa agcgatgctt gcgggtatcg
      481 gtgaaaacgc cggcgtcgtc gacatcggcg acggctgggc cgtcacgttc aaggtcgagt
      541 cgcacaacca cccgtcgtac gtggagccgt accagggtgc ggccaccggg gtgggcggca
      601 tcgtgcgcga catcatggcg atgggcgcgc ggcctgtcgc ggtgatggac cagctgcggt
      661 tcggcgctgc cgacgccgcc gacacccggc gggtggtcga cggcgtcgtg cgcggtatcg
      721 gcggctacgg caactcgctt ggcttgccca atatcggtgg cgagacggtg ttcgacgcgt
      781 cgtatgccgg caacccgctg gtgaacgcgc tgtgcgtcgg ggcgctgcgc aaagaggacc
      841 tgcacctggc tttcgcatcc ggcaccggca acaagatcat cctgttcggc gcccgcaccg
      901 ggctggacgg gatcggcggc gtgtcggtac tcgcgtcgga gacgttcggc ggcgacgagt
      961 ccgggtcagg taggaaaaag ctgccgagcg tgcaggtggg cgacccgttc atggagaagg
     1021 tgctcatcga gtgctgtctg gagttgtacg ccgcggacct ggtggtcggc atccaggacc
     1081 tgggtggtgc cggattatcc tgcgccacat cagaactcgc gtctgcgggt ggtcaaggta
     1141 tggccgtcga gctggacaac gtgccattgc gaaccgccaa tatgacgccc gcggaggtgc
     1201 tcgccagcga gtcgcaggaa cgcatgtgcg cggtggtcaa gcccgagaac gtggacgcgt
     1261 tcctcgcggt gtgccgcaag tgggaggtgt tggccaccgt gatcggcgag gtcaccgacg
     1321 gcgaccggct gcggatcacc tggcacggcg aggcggtggt cgacgtgccg ccgcggacag
     1381 tggcccacga ggggccggtc tatcagcgtc ccgtaaagcg acccgacagc caggacgcac
     1441 tgaacgccga cacctcggcg cggctgccgc ggccgaccac cggcgacgag ttgcgcgcga
     1501 ctttgcttgc gctgcttggc agcccgcacc tgtgcagccg cgcgttcatc accgagcagt
     1561 acgaccgcta cgtccgtggc aacacggtgc tcgccgagca cgccgacggt ggcgtattgc
     1621 gcatcgacga acggaccggc cgcggtatcg ccgtgtccac cgatgcaaac ggccgctaca
     1681 cccagctcga cccgtacacg ggcgcgcagc tcgcgctggc cgaggcctac cgcaacgtcg
     1741 cggtcaccgg cgccacgccc gtggctgtca ccaactgcct caacttcggg tctccggagg
     1801 atcccggcgt gatgtggcag ttctcgcagg ctgttcgtgg cttggccgac ggctgtgcgg
     1861 aacttggaat cccggtcacc ggcggcaacg tcagcttcta caaccagacc ggctccaccg
     1921 cgatcctgcc gacccccgtc gtgggggtac tcggggtgtt ggaagacgtg agccgacgta
     1981 ttcctaccgc tttcggtacc gagccgagta agacactgat gctgctcggc gacactcgcg
     2041 acgagttcga cgggtcgatc tgggcgcagg tcaccgcgga ccacctgggc ggccgccccc
     2101 cggccgtcga cctgcag
//
LOCUS       DASWBF010000030         6780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_790822,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000030 DASWBF010000000
VERSION     DASWBF010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790822"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF874_00810"
     CDS             complement(<1..284)
                     /locus_tag="VF874_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005240141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H)"
                     /protein_id="HEX9174678.1"
                     /translation="MTRKSLVVVGHGMVGHRFVEAMRDRDRDGRWHIIVLCEEPTPAY
                     DRVALSSYVGSWDRAALALPGNDYAADGFVELRTDVRATAIDRDARVVTTS"
     gene            complement(281..1711)
                     /locus_tag="VF874_00815"
     CDS             complement(281..1711)
                     /locus_tag="VF874_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016343152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX9174679.1"
                     /translation="MPTRTRKHDIDNWDPEDVDAWNNGGANIARRNLIWSVITEHVGF
                     SVWSIWSVMVLFMPQAVYHIDAAGKFFLVAVPTLVGAVMRIPYTFAVAKFGGRNWTVF
                     SALVLLIPTLMTLYFMAHPETSYTAFMAVAALAGFGGGNFASSMTNINAFYPQRHKGW
                     ALGLNAGGGNIGVPVIQLVGLLVIATVGNRSPHWVCAVYLVLIAIAGLGAALFMDNLT
                     HQKTNAKSMVEVLGYRDSWLIALLYIGTFGSFIGFSFAFGQVLQMNFLAGLTDGGPAT
                     AAMTAQASLHAAQIAFIGPLLGSLSRPPGGWLSDRIGGGKVTLYTFAAMIAAAGWLVV
                     AGRLDDATPGAPSGGIMTAYVIGFIVLFILSGIGNGSVYKMIPSIFAAKAQSRDDLSA
                     AEKTAWSRTMAGALIGIAGAVGALGGVGINLVLRASYLSAAHSATMAFWVFLGCYVLC
                     ATITWFAYVRRPVRVPALATPEAVPA"
     gene            complement(1805..2659)
                     /locus_tag="VF874_00820"
     CDS             complement(1805..2659)
                     /locus_tag="VF874_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02569 family protein"
                     /protein_id="HEX9174680.1"
                     /translation="MTAERPPEHVQAAFGLTGFRPVPLRAGWEGGFRCGEVVLSMVAD
                     HARAAWSAKVRETLFVDGVRLARPVRSTDGRYVVSGWRADTFVAGTPEPRHDEVLSAG
                     VRLHEATAKLERPRFLTQPPVAPWGDVDVFIAADRAAWEDRPLHAWPSGARVSPGSAD
                     GQRSLDLINQLTGLRRPTKSPNQLVHGDLYGTVLFAGTAAPGITDITPYWRPAAWAAG
                     VVVVDALSWGEADDGLIERWAPLPEWPQMLLRALMFRLAVHALHPRSTAAAFPGLART
                     AALVRLIL"
     gene            complement(2715..3893)
                     /gene="moeZ"
                     /locus_tag="VF874_00825"
     CDS             complement(2715..3893)
                     /gene="moeZ"
                     /locus_tag="VF874_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/sulfurtransferase MoeZ"
                     /protein_id="HEX9174681.1"
                     /translation="MSTTLPPLVQPAAELTREEVARYSRHLIIPDLGVDGQKRLKNAR
                     VLVIGAGGLGSPALLYLAAAGVGTIGIVEFDVVDESNLQRQIIHGQSDIGRSKAESAR
                     DSIREINPLVDVRLHELRLEPTNAVELFEQYDLILDGTDNFATRYLVNDAAVLAGKPY
                     VWGSIYRFEGQVSVFWEDAPDGLGLNYRDLYPEPPPPGMVPSCAEGGVLGILCASIAS
                     VMGTEAIKLITGIGEPLLGRLMVYDALEMTYRTIKIRKDPETVKITELIDYEEFCGVV
                     SDEAAEASADSTVTPRELRELLDSGKKVALIDVREPVEWDINHITGAQLVPKSTIESG
                     DGLAKLPQDGIAVLYCKTGVRSAEALAAVKKAGFSDAKHLQGGIVAWAKQLEPDMVMY
                     "
     gene            complement(4015..5049)
                     /locus_tag="VF874_00830"
     CDS             complement(4015..5049)
                     /locus_tag="VF874_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HEX9174682.1"
                     /translation="MTYDPGRRGGRVPVLRNEWREPLRAQRDPVSVDYGRVRSNRDDR
                     QRWRKRTWLGRFVSTYGWRAYALPVLAAVTVMALITMFDGSAQDKATSDGVVEGPRQT
                     IEAVGTAIIGAPPKGLTQFDASLPTGVLPDGGPYTQGGAKTWHIVPGNEQQVGQGTTK
                     VFTYTVEVEDGVDTTSYGGDEGFARMVSQTLANPKSWTHNTQFAFRRVDGSAQGNPDF
                     RVSLTSPMTVREGCGYEIPLEASCYNPAYGPEAQRRVFMNEARWVRGAVPFQGDIGSY
                     RQYLINHEVGHAIGYQRHEPCAENGDLAPVMMQQTFSINNNDEFRFDPDTVKPDGKVC
                     RFNPWPYPIA"
     gene            5161..5826
                     /locus_tag="VF874_00835"
     CDS             5161..5826
                     /locus_tag="VF874_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9174683.1"
                     /translation="MSDLANTAERRGDAVSAPHGRARRGSRLPRDERRGQLLAAASEV
                     FVDRGFHAAGMDEIADRAGVSKPVLYQHFSSKLELYLAVLQRHVENLVSGVRQALRTT
                     TDNRQRLRAAVQAFFDFIEHDSQGYRLIFENDYVSEPQVAAQVRVATESCTDAVFDLI
                     SRDSGLEAHRARMIAVGLVGISVDCARYWLGAERPITKDAAVEGAVQFAWGGLSHVPL
                     ARS"
     gene            complement(5829..6083)
                     /locus_tag="VF874_00840"
     CDS             complement(5829..6083)
                     /locus_tag="VF874_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEX9174684.1"
                     /translation="MQQRKGCSSVEVKIGISESPRELVLNSSQTPAEVEEEVRAALEK
                     GSPVLDLTDERGRKVLVQTSKISYVEIGVADVRRVGFGGG"
     gene            6250..>6780
                     /locus_tag="VF874_00845"
     CDS             6250..>6780
                     /locus_tag="VF874_00845"
                     /inference="COORDINATES: protein motif:HMM:NF017260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HEX9174685.1"
                     /translation="MTRPYRGDTSGAGPSTATRELVRDDYDDDVVFYQDSSPWRWAAA
                     VGGVVLVLAVILAILIVRGGDGSSTMTGVVPSRSAPVLTPPAATSRPSPPPTASLAPE
                     TVSTVTPSPTITPSPTEAVPTAATPQADPRTVTYTVSGSRQPGDIVTVTYLDETGLPR
                     TDFNVTLPWSKTIVSGG"
BASE COUNT         1142 a         2365 c         2197 g         1076 t
ORIGIN      
        1 gaagtagtga cgacgcgcgc atcgcggtcg atcgccgtcg cccgcacgtc ggtgcgtagt
       61 tcgacgaatc cgtctgcggc gtagtcattt ccgggtagcg ccaacgcggc acgatcccag
      121 ctgccgacat acgaggacag cgccacccgg tcgtaggccg gggtcggctc ctcacacagc
      181 acgatgatgt gccagcgccc gtcgcggtcg cggtcgcgca tcgcctcgac gaaccgatgc
      241 cccaccatcc cgtgaccaac aaccacaagg cttttgcgcg tcatgccggc accgcttccg
      301 gtgtcgccaa tgccggcact ctgacgggcc ggcgcacgta cgcgaaccat gtgatcgttg
      361 cgcacagcac gtagcagccg aggaacaccc agaaggccat cgtggccgag tgcgccgccg
      421 acagatacga ggcgcgcagg acgaggttga tcccgacacc gccgagagcg ccgacagctc
      481 cggcaatccc gatcaacgcg ccggccatgg tgcgcgacca cgccgtcttt tccgcagcgc
      541 tcagatcgtc gcggctctgc gccttggcgg cgaagatcga cggaatcatc ttgtacaccg
      601 agccattccc gatgccggac aggatgaaca gcacaatgaa tccgatgacg taggcagtca
      661 tgatgccgcc ggagggggca ccgggcgtcg cgtcgtcgag acgcccggcg accaccagcc
      721 acccggcggc ggcgatcatc gcggcgaacg tgtacagcgt caccttgccg ccaccgatcc
      781 ggtcggacag ccagccaccg ggtgggcgcg acagtgagcc caagagcggt ccgatgaatg
      841 cgatctgagc ggcgtgcagc gaggcctgcg cggtcatcgc cgcggtggcg ggaccgccgt
      901 cggtgagccc ggcgaggaag ttcatctgca acacctggcc gaacgcgaac gagaatccga
      961 tgaacgaacc gaaggtgccg atgtagagca gcgcgatcag ccacgaatcg cggtagccca
     1021 gcacctcgac catcgacttg gcgttggtct tctggtgggt gaggttgtcc atgaacagcg
     1081 ccgcgcccag ccccgcgatc gcgatgagca ccagatacac cgcgcacacc cagtgtggcg
     1141 atcggttgcc gacggttgcg atcaccagca gcccgacaag ctggatcacc ggcacgccga
     1201 tattgccgcc gccggcattc agccccagcg cccagccctt gtgccgctgc ggatagaagg
     1261 cgttgatgtt cgtcatcgac gaggcgaagt tgccgccacc gaagcctgcc agcgccgcca
     1321 ccgccatgaa cgcggtgtag gacgtttccg gatgcgccat gaaatagagc gtcatcagcg
     1381 tcgggatcag cagcaccaac gccgagaaca cggtccagtt gcggccgccg aacttggcca
     1441 ctgcgaacgt gtacgggatt cgcatcaccg cgccgaccag ggtcgggacc gccaccagga
     1501 agaacttgcc ggcggcatcg atgtggtaga cggcctgcgg catgaacagc accatcaccg
     1561 accagataga ccagaccgaa aagccgacgt gttcggtgat caccgaccag atcaggttgc
     1621 ggcgggcaat gttcgctccg ccgttgttcc aggcgtccac gtcctcgggg tcccagttgt
     1681 cgatgtcgtg cttgcgtgtg cgggtgggca tgcggccacg gtaggaaagc cttgttgcgg
     1741 aaacgatgca ggcgatgacc cgagtgatac atcctgctca catgccccgc gcggggccgg
     1801 tgagctaaag gatcagccgg acaagtgcgg ccgttcgggc caggcccgga aacgccgcgg
     1861 cggtggatcg tggatgcagc gcgtgcacgg caaggcgaaa catcaacgcg cgcaacaaca
     1921 tctgcggcca ttcgggcagc ggcgcccatc gttcgatcag cccgtcgtcg gcctcgcccc
     1981 acgacagagc gtccacgaca accacacccg ccgcccacgc cgccggccgc cagtacggtg
     2041 tgatgtcggt gatgcccggt gccgccgtgc ccgcgaaaag cactgtgccg taaaggtctc
     2101 cgtgcaccag ttggttgggg ctcttggtcg gtctgcgcag cccggttagc tggttgatca
     2161 gatcgagcga gcgctggccg tccgccgatc cgggcgacac ccgcgcgccc gacggccagg
     2221 cgtgcagcgg gcggtcctcc cacgcggcgc ggtcggcggc gatgaacacg tcgacgtcgc
     2281 cccacggcgc gaccggcggc tgcgtgagaa accgcggccg ttcgagtttc gcggtggcct
     2341 cgtgcaggcg aacccccgcc gacaggacct cgtcgtgccg cggctcaggc gtgcctgcga
     2401 cgaaagtgtc tgcgcgccag cctgatacca cgtagcgccc gtcggtcgag cgcaccggcc
     2461 gggccagccg cacgccgtcg acgaacaacg tctcgcgcac cttcgcggac cacgcggcgc
     2521 gggcatggtc ggcgaccatc gacagcacga cttccccgca gcggaacccg ccttcccagc
     2581 cggcccgcag cggcacgggg cgaaatcccg tcaacccgaa cgccgcctgc acatgctccg
     2641 gcggccgttc ggcggtcatg gcgtcaggtt acctgcgtaa acgcggcgac ccgtagggct
     2701 gacgggccgt gtcgtcagta catcaccatg tcgggctcga gttgcttggc ccacgcgacg
     2761 atgccacctt ggaggtgctt tgcgtcggag aagccggcct tcttcaccgc ggcgagcgcc
     2821 tcggcggagc gcacaccggt cttgcagtac agcaccgcga tcccgtcctg cggcagcttg
     2881 gccagtccgt cgcccgactc gatcgtcgac ttgggcacaa gctgtgcgcc cgtgatgtga
     2941 ttgatgtccc actcgacggg ctcacgcacg tcgatcagcg cgaccttctt gccggagtcg
     3001 agcagttcgc gcagctcgcg cggcgtcact gtggagtccg ccgacgcctc ggctgcctcg
     3061 tcggacacga cgccgcagaa ctcctcgtag tcgatgagct cggtgatctt gaccgtctcg
     3121 gggtccttgc ggatcttgat cgtgcggtag gtcatctcca gcgcgtcgta caccatcagc
     3181 cggcccagca gcggctcgcc gatgccggtg atcagcttga tcgcctcggt gcccatcacc
     3241 gacgcgatcg acgcgcacag aatccccagt accccgccct ccgcgcacga aggcaccatg
     3301 cccggtggcg gcggctcggg gtacaggtcg cggtagttga gaccgagccc gtccggcgcg
     3361 tcctcccaga acaccgacac ctggccctcg aagcggtaaa tcgatcccca gacgtacggc
     3421 ttgccggcga gcaccgccgc gtcgttgacc agataacggg tggcgaagtt gtcggtgcca
     3481 tcgaggatca ggtcgtactg ctcgaacagc tccaccgcgt tcgtcggctc caaccgcagc
     3541 tcatgcagcc ggacgtcgac aagcgggttg atctcccgaa tcgaatcgcg agcgctttcc
     3601 gccttggacc ggccgatgtc ggactggccg tgaatgatct ggcgctgaag gttggactcg
     3661 tcgacgacgt cgaactcgac gatgccgatg gtgccgaccc ccgcggcggc caggtacagc
     3721 aatgccggcg agccgagacc accggcaccg attaccagca cgcgtgcgtt cttcaggcgt
     3781 ttctgcccgt cgacgccgag gtcgggaatg atgagatgac ggctgtagcg cgcgacctct
     3841 tcacgggtca actcagcggc tggctgcacc agcggcggca aggttgtcga cacgcggtct
     3901 ctcctcggtc agcggttcac accatcacaa taggcacgtc gaataacgac gtagcccgct
     3961 cagaccttcc cgaatccccg cgcgagcgtg cgctcaatgc cactaacacc ggggtcaggc
     4021 gatgggatac ggccaggggt tgaagcggca caccttgccg tcgggcttga ccgtgtcagg
     4081 gtcgaaccgg aactcgtcgt tgttgttgat ggagaacgtc tgctgcatca tcaccggcgc
     4141 caggtcgccg ttctcggcgc acggctcgtg ccgctggtag ccgatcgcgt ggccgacctc
     4201 gtggttgatc aggtactgcc gataggagcc gatgtcgccc tggaacggca ccgcgccacg
     4261 cacccagcgc gcctcgttca tgaacacccg tcgctgcgcc tccgggccgt aggccgggtt
     4321 gtagcacgac gcctccagcg gaatctcgta cccacagccc tcgcgcaccg tcatcggcga
     4381 cgtcagcgac acccggaaat ccgggttgcc ctgcgcactg ccgtcgacgc ggcggaacgc
     4441 gaactgggtg ttgtgcgtcc agctcttcgg gttggccaga gtctggctga ccatgcgcgc
     4501 gaagccctcg tcaccgccgt aggacgtggt gtcgacgccg tcctccacct cgacggtgta
     4561 ggtgaagacc ttggtggtgc cctggccgac ctgttgctcg ttgcccggca cgatgtgcca
     4621 cgtcttcgca ccgccctgcg tgtacgggcc gccgtcgggc aacacgccgg tgggcaggct
     4681 cgcgtcgaat tgcgtcagcc ccttcggcgg cgcgccgatg atcgccgtgc cgaccgcctc
     4741 gatcgtctgc cggggaccct ccacgacgcc gtcgctcgtg gctttgtctt gcgcgctgcc
     4801 gtcgaacatg gtgatgaggg ccatgaccgt gacggccgcc agcaccggca acgcatacgc
     4861 acgccagccg tacgtcgaca cgaaccggcc gagccatgtc cgcttgcgcc accgctgtct
     4921 gtcgtcacga ttcgacctga cccgcccgta gtcgacggac accgggtcgc gctgggcacg
     4981 caacggctcc cgccactcgt tgcgcaacac cggcacacga ccgccgcgac gccccgggtc
     5041 gtaggtcacg gaacacagaa tgtcacagtc gatacgggtc gcacttccgg cgcaccgctg
     5101 cctgcggtag tagtgtcgga ctcttggacg aagccagaca gcgaccagat gaggatggtg
     5161 atgagcgatc tcgccaatac cgccgaaagg cggggcgacg cggtcagcgc ccctcacggt
     5221 agggctcggc gcggcagccg gcttccccgt gacgagcggc gcggacagtt acttgccgct
     5281 gccagtgaag ttttcgtcga ccgcggcttc cacgcggcag gcatggacga gatcgccgat
     5341 cgcgccggcg tcagcaaacc cgttctgtac caacacttct cgagcaaact cgagctgtac
     5401 ctggccgtcc ttcaacggca cgtcgagaac ctggtgtccg gcgtccggca ggcgctgcgg
     5461 accaccacgg acaaccggca gcggctacgg gcagcggttc aggcattctt cgacttcatc
     5521 gagcacgaca gccagggcta ccggctgatc ttcgagaacg actacgtctc cgagccgcag
     5581 gtggccgcgc aggtccgggt tgccacggag tcgtgcaccg atgcggtgtt cgacctgatc
     5641 agccgcgact ccggcctgga ggcgcatcgc gcccggatga tcgccgtcgg gctggtcggc
     5701 atcagcgtgg actgcgcccg ctactggctg ggcgccgagc ggccgatcac caaagacgcc
     5761 gccgtcgagg gcgcggtgca gttcgcctgg ggtgggttgt cgcacgtgcc gctggcgcgg
     5821 tcgtagcgct agccgccgcc gaacccgacc ctgcgcacgt cggccacgcc gatctccacg
     5881 taggagattt tggacgtctg caccaaaacc ttgcgtcccc gctcgtcagt caggtcgagc
     5941 accggtgagc ccttctccag cgcggcgcgg acctcctctt cgacctcggc gggcgtctgc
     6001 gagctgttga gtaccagctc gcgcgggctc tccgagatgc cgatcttgac ctccacgctg
     6061 ctgcaccctt tccgttgctg cattctcccc cgacagcagg ttagtggaag ggtcacagac
     6121 cagccggatt gcgggcgttc gcgctgcgcg aatcgacacc catcggtgac ctgacacgcc
     6181 cggcggccga ccggaaccgg cctcgcgaga ccgttaccat cgtgcgttcc gtgagtgagg
     6241 tgagtatcga tgaccaggcc gtatcgcggc gacacctcgg gcgctggccc ctccaccgcg
     6301 acccgcgagc tcgtccgcga cgactatgac gacgacgtcg tcttctacca ggattcgtcc
     6361 ccctggcgct gggcggctgc ggtgggcggc gtcgtgctgg tgctcgccgt gatccttgcg
     6421 atcctcatcg tgcgtggcgg cgacggctcg tcgacgatga cgggcgtcgt gccgtcgagg
     6481 tcggcgcccg tgctgacgcc gcccgccgcg acatcacggc cctcaccgcc gccgaccgcg
     6541 tcgctggcgc ccgagacagt gagtacggtg acgccgtcgc cgacgatcac gccgtcgccc
     6601 accgaggccg tcccgacggc ggctaccccg caggccgatc cccggaccgt cacgtacacg
     6661 gtctcaggca gccgccagcc gggcgacatc gtcacggtga cctacctcga cgaaacgggc
     6721 cttccgcgga ctgacttcaa cgtcacgctg ccgtggagca aaaccatcgt ctcgggcggc
//
LOCUS       DASWBF010000031         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_725972,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000031 DASWBF010000000
VERSION     DASWBF010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725972"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..331
                     /locus_tag="VF874_00850"
     CDS             38..331
                     /locus_tag="VF874_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174686.1"
                     /translation="MSVRVPSLPCYLVEWYRPEITAVDLDETVARLDASAAMMSAAGA
                     PVSLMMTLALPTDEVVFCVFSAGSAQFVADACCRAGFPPERLTQADGARIAGG"
     gene            371..636
                     /locus_tag="VF874_00855"
                     /pseudo
     CDS             371..636
                     /locus_tag="VF874_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007240635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            661..915
                     /locus_tag="VF874_00860"
     CDS             661..915
                     /locus_tag="VF874_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9174687.1"
                     /translation="MSSPLRLARALHRLPAKAAAAIIEFITGPLAADPHRFSKPLVNE
                     LSAYRSASRGDYRVLIRIDEAKRRVLVAYVDHRADVYRRR"
     gene            969..2306
                     /locus_tag="VF874_00865"
     CDS             969..2306
                     /locus_tag="VF874_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5933 domain-containing protein"
                     /protein_id="HEX9174688.1"
                     /translation="MFIGDAGLVQWRLPSTRTRAGAVVVLIALLLVVLQIAASRGGFQ
                     GPLHSLISDYVATPKSATVPWVGLGVAMVGLSHHWRIRALSLAAVLDVLFAAERLLRG
                     GVFAVGNGPVIVLTGLTVLALTRWTGEQRSNALRGIAFGILLILATKVGDAWLRITAI
                     ARPTVWDKYALLADHALAQPSWLLGRLVDTAGPVASGLLHWVYIELPAAALVAALYQL
                     RHVTTVGWPCHFLVRSFLVLGLVGPVIYILFPIVGPAYAFSAAGEGFQVGDYWSHALP
                     PIDMAPAAIAFDNETARNCMPSMHTAWALAVFLHTRGGPRWLRWGGTFWLAGTLAATL
                     GFGYHYGVDLIAGAVLCMTVESALREPQWGGNSSRVWLVAAGSSFLAALLLACRYLAE
                     PMARYPLLFGPLLIGALVVFSGTFYATFFSQTWLGIALRPAAAQRSQPQAEYG"
     gene            2343..>2722
                     /locus_tag="VF874_00870"
     CDS             2343..>2722
                     /locus_tag="VF874_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX9174689.1"
                     /translation="MFEPVVSSLLDTFSGVEDAGVVAAIGVASGEENAACARRLEAMG
                     ELYSRRAPEDDGERIGWLVDGYESLAAEVAAELGISRGWARGQLRYAIHLREKLPQLM
                     RVFVTGAIDMRMVITIVNRVVLIT"
BASE COUNT          385 a          852 c          944 g          541 t
ORIGIN      
        1 atgacggcga cagcttgcgg tctctagcgt cgaggccgtg agcgttcgcg tcccaagcct
       61 gccgtgttac ttggtcgagt ggtaccggcc cgagatcact gccgttgatc ttgacgagac
      121 cgtggcgcgg ctcgacgcga gtgctgcgat gatgtcggcc gctggggcac cagtcagctt
      181 gatgatgacg ctcgcactgc ccacggacga ggtggtcttt tgcgtgttct cagccgggtc
      241 agcacagttc gttgccgacg cgtgttgccg ggccggtttc ccgccggagc gtctcacgca
      301 ggccgatggc gcccggatcg ccggcggatg aagtagccac acttttggct ataatcacgc
      361 catgtagtca gtgccgctcg gtgaggcgaa ggatcgactg tccggtcttg tcgatgaggc
      421 ggagtcaccc acgagatcat caccatcacc aagcatggcc gtgtagcagc ggtactgatg
      481 agcgcggacg acctcgaatc tctgcacgag acgctggact ggttggccaa gcccggcatc
      541 gctgacccca tcgcagccgc cgagcgcgaa tacgaggccg gcgaaacgat cagcggagac
      601 gaattgcgag ccgagtttgg tctttcgtcg cagtgaactc cggcgacgaa ccgtacagga
      661 ttgagctcgc cgcttcggct cgcccgagcc ttgcatcgcc ttcctgccaa ggcggccgct
      721 gcaatcatcg agttcatcac cggtccgctt gccgcagacc cccaccgctt cagcaagccg
      781 ctcgtcaacg agctgagtgc ataccgcagt gccagccgcg gcgactatcg agtgctgatc
      841 cgcatagacg aagccaagcg ccgcgtcctg gtcgcctacg tcgaccaccg cgcggatgtc
      901 taccgacgtc gttgacggct ctctcgggtt agctctgagt cgctggtccg ccatctacta
      961 ttcaacaggt gttcatcggc gacgcaggac ttgtgcagtg gcgcttgccg tcgactcgca
     1021 ctcgggcggg cgccgtcgtc gtgctgatcg cgctgctgtt ggttgtgttg cagattgccg
     1081 cgagccgagg cggatttcaa ggcccactgc acagcctgat cagcgactac gtcgcaaccc
     1141 cgaaatcggc gacggtgccg tgggtcggcc tgggcgtggc gatggtcggc ctgtcccacc
     1201 actggcggat ccgggcgctg tcgctggccg cggtcctcga tgtgcttttc gccgccgagc
     1261 ggctgttgcg cggcggggta ttcgccgtgg ggaacggccc ggtgatcgtg ctcaccggct
     1321 tgacggtgtt ggcgttgacg cgatggacgg gcgagcagcg cagcaacgcg ttgcgcggca
     1381 tcgcattcgg catcctgctc atcctggcga ccaaggtcgg cgatgcgtgg ttgcgtatca
     1441 cggcgattgc ccggccgacg gtctgggaca agtacgcgct gctggccgat catgcgctgg
     1501 cgcagccgtc ctggttgttg ggccggctgg tcgacaccgc cgggcccgtt gcgtccggtc
     1561 tgctgcactg ggtgtacatc gagttgcccg ccgcggcgct cgtggccgcg ctctaccaat
     1621 tgcgtcatgt caccacggtc ggctggccgt gccacttcct agtgcgctcg tttctggtgc
     1681 tgggcctggt cggcccggtg atctacattc tgtttccgat cgtcggtccc gcttacgcat
     1741 tcagcgctgc gggcgaggga tttcaagtcg gcgactactg gtcccacgcc cttccgccca
     1801 tcgacatggc gccggcggcc attgcattcg acaacgaaac cgcacgcaac tgcatgccgt
     1861 cgatgcacac cgcctgggcg ctggccgtgt tcctccatac ccgtggcgga ccccgctggc
     1921 tgcgatgggg cggcacgttc tggctggcgg gcacgctcgc ggcgacgctg ggcttcggct
     1981 accactacgg cgtcgacctc atcgccggag ccgtgttgtg catgaccgtg gaatccgcgc
     2041 tgcgggaacc acagtggggc gggaactcgt cgcgggtgtg gctggtcgcc gccggctcgt
     2101 cgttcttggc ggcgctgctg ttggcctgtc gctatctcgc tgagccgatg gcgcgatacc
     2161 cgctgctgtt cggcccgttg ctcatcgggg cgctggtggt gttcagcggc accttctacg
     2221 cgaccttctt cagccagacc tggctcggca tcgcgctgcg tcccgctgcc gcgcaacggt
     2281 ctcagcccca ggccgagtac ggctgacgcg gcttgccggt gcaggctggc atactcgaag
     2341 gtatgttcga accggtggtg tcgtcgctgc tagacacgtt ttctggggtg gaggacgccg
     2401 gtgtggtggc ggcgatcggg gtggcgtctg gggaggagaa cgcggcgtgt gcgcgccggt
     2461 tggaggcgat gggggagttg tattcgcgcc gggcgcccga ggacgacggc gagcgcatcg
     2521 gctggttggt cgacgggtat gagagtctgg cggccgaggt ggccgcggag ttgggcatca
     2581 gccggggctg ggcgcgcggg cagctgcggt acgcaatcca tctgcgcgag aagctgccgc
     2641 agctgatgcg ggtgtttgtc accggcgcta tcgatatgcg gatggtgatc accatcgtca
     2701 accgggtggt gttgatcacc ga
//
LOCUS       DASWBF010000032         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_68368, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000032 DASWBF010000000
VERSION     DASWBF010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68368"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1885
                     /locus_tag="VF874_00875"
     CDS             371..1885
                     /locus_tag="VF874_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9174690.1"
                     /translation="MRAEVEELRASEAAVRADHAELKSMVAVLTGQVAQLERVVREQA
                     DEIAELKRQAAGDSSNSSRPPSSDAPWAKKPAKMRSSRTRSGRKSGKQPGSASSSRCL
                     IDDPDERLEIRPGRCRSCDASLVEATEHGHRRRQIVDIQPAPPPKVTEYVRISKVCAC
                     CGVVTTPGWDDEAVPVEHVGIVAAPGSPVRIGPETLARAALLTCAHYLPVGRARDLLE
                     TLTAIDVSTGFLAAIRGRAARQLEKKFLGHMRNLLATAPVLHADETPGRATGSLAYVH
                     VACTEYLTLMHVGDRSAASIDAGGVLGQFTGVLVRDGYAGYAHLQAAHAWCGAHLLRD
                     LRSISDADPDGQLWAQAMANTLLEANRAAHEARDRGADRLDEATLKQIRNHYLGALAR
                     GNTDNQGEHSTLADKARTLITRFRRFEDMILRFAADLSVPFTNNEAERACRPVKIQQR
                     TSGGCWRTLQGLTDFAIVQSYLDTATKWGLDKLDALRQLFTTGAWLPPTLKPAE"
BASE COUNT          338 a          678 c          666 g          318 t
ORIGIN      
        1 gcccacttac tgaaaagatt aggttaccac actcaacctg ccgtaagtga gccgccgaga
       61 atgtactata ccaccgggtt ctgcactggc gaaatcaagg tagctattca gctgttgcca
      121 ctcacctcgg cggcacgttc cgcgccaccg cgagcgcgta gacgggcggc gcgtcgacgg
      181 tcatccgtcg gcggacgggt cccagcattg cgagcggcgg cggcgtgcgc gttgtggctg
      241 gtgtgacgtg atcgtcggcg tgtcgagttg atctcggtgt cgggttgggc agggtgtcgt
      301 cgtgcccgat cgcccgcccg acgagcagtc ggctgtggac gacgctgcgc tgcgggtgga
      361 gaacgccgcg ctgcgtgctg aggtggagga gcttcgggcg agcgaggctg cggtgcgtgc
      421 cgatcacgcc gagttgaaga gcatggtcgc ggtgttgacc gggcaggttg cgcagctgga
      481 gcgggtggtc agggaacagg ccgatgagat cgcggagttg aagcgccagg ccgctgggga
      541 ctcctcgaac tcctcccgcc ctccgtcctc ggatgcgccg tgggcgaaga agccggcgaa
      601 gatgcgctcg tcgcggacga ggtcgggacg caagtccggc aagcagccgg gctctgcgtc
      661 gtcctcgcgc tgcctgatcg acgacccgga cgagcggctg gagatccggc cgggccgctg
      721 ccggtcctgt gacgcgtcac tggtcgaggc caccgaacac ggccatcggc ggcgtcagat
      781 cgtcgacatc cagcccgcgc cgcccccgaa ggtcaccgag tatgtgcgga tctcgaaggt
      841 ctgcgcgtgc tgcggggtgg tcaccacccc gggctgggac gacgaggccg tgccggtcga
      901 gcacgtcggc attgtggccg cgccgggctc gccggtgcgg atcggcccgg aaaccctcgc
      961 ccgcgccgcg ttgctcacct gcgcgcacta cctgccggtc gggcgcgccc gcgacctgct
     1021 ggagacgttg accgcgatcg atgtgtccac cgggttcctg gccgccatcc gtggtcgcgc
     1081 cgcccgacag ttggagaaga agttcctcgg ccacatgcga aacctgctgg ccaccgcgcc
     1141 ggtgctgcat gccgacgaga cacccggccg cgccaccgga tccctggcct acgtccacgt
     1201 ggcctgcacc gaatacctca cgttgatgca cgtcggcgac cgctccgcgg cgagcatcga
     1261 tgccggcggt gtcctcggac agttcaccgg ggtgctggtg cgcgacggct acgccggtta
     1321 cgcacacctg caggcggcac atgcctggtg cggagctcat ctgctgcgcg atctgcggtc
     1381 gatctccgat gccgaccccg acggccaact gtgggcgcag gcgatggcca acaccctgct
     1441 ggaagccaac cgcgccgccc acgaggcccg tgatcgtggc gccgaccgcc tcgacgaagc
     1501 cacgctgaag cagatccgca accactacct gggagcactc gcccgaggca acaccgacaa
     1561 ccagggcgaa cactccacgc tcgccgacaa ggcccgcacc ctcatcacgc ggttcagacg
     1621 gttcgaagac atgatcttga ggtttgccgc ggatctctcg gtgccgttca cgaacaatga
     1681 agcggagcgc gcgtgcaggc ccgtcaagat ccaacaacgc acctccggcg gctgctggcg
     1741 caccctgcaa ggcctcaccg acttcgccat cgtccagtcc tacctggaca cagccaccaa
     1801 atggggactc gacaaactcg acgccctccg ccaactcttc accaccggcg catggctacc
     1861 acccacactc aaaccagctg aatagctacg gcgggagggt tggggatcat gacgcaattg
     1921 ccgcaatctg ctcctattag tcggagcatg tggaagccgg tcgtcggcct gttgatcagg
     1981 gtcactgtct cggcaacagt
//
LOCUS       DASWBF010000033        14665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_398065,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000033 DASWBF010000000
VERSION     DASWBF010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14665
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398065"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF874_00880"
     CDS             complement(<1..180)
                     /locus_tag="VF874_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174691.1"
                     /translation="MLVVTACLYLWNLSASGWANGFYSAAVQAGSQSRTAFLFGSSDA
                     ANAITVDKTPAALWVM"
     gene            complement(294..1856)
                     /locus_tag="VF874_00885"
     CDS             complement(294..1856)
                     /locus_tag="VF874_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9174692.1"
                     /translation="MSSNLPTDARRSARLRPRAWSLRTRLLVTVVTLLAVVCVGIGVG
                     TELALHRFLMRQLDERVVDAGRRSEAIFDLGLPLSPPPGYPRRPPAFDRHSGPGPMFL
                     DAPGQATGTVGAVMRDGTVLDAGVITQNGTRESISNAAAAQLSRTTPDGRPVTVNLDG
                     LGRYRVIARHSRSGDTVVSGLPMAEVDNTLLSVGTIFGIVAGIALIAAIAAGIVIIRR
                     QLAPVSRVAATARDVANLELDRGEVTLPTHVLAVDPAAAHTEVGQLAAALNRMLERIS
                     DALAARHASETRVRQFVADASHELRTPLSAIRGYTELAQRRRGDVPADVAHAMGRVES
                     EAARMTQLVEDMLLLARLDTGRPLQRERVDLSQLAVDAVADAHVAGPEHRWSLDLPDE
                     PVVVTGDGQRLQQVLVNLLANARTHTPAGTSVTTSLASAGAGGAVLTVADDGPGIPAA
                     VRPEIFERFARGDTSRSRRDGSTGLGLAIVSAVVKAHHGSIDVRSVPGDTAFTVRLAA
                     SQPAPSDDKSGA"
     gene            complement(1834..2538)
                     /locus_tag="VF874_00890"
     CDS             complement(1834..2538)
                     /locus_tag="VF874_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9174693.1"
                     /translation="MRRADGSPIHVLVVDDEQVLAELVTMALRYEGWTVSTAGDGASA
                     IAAARKDAPDVVVLDVMLPDMSGLDVLRKLREQLPGLPLLLLTAKDAVEDRIAGLTAG
                     GDDYVTKPFSLEEVVLRLRALLRRTGVTTETSGARIVVGDLVLDEDSHEVTRGGDAIG
                     LTTTEFELLRFMMRNARKVLSKAQILDRVWSYDFGGRSNIVELYVSYLRKKIDNGRAP
                     MIHTLRGAGYVLKPAD"
     gene            2750..3313
                     /locus_tag="VF874_00895"
     CDS             2750..3313
                     /locus_tag="VF874_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174694.1"
                     /translation="MSSDTNTADETVWGAPQPGRKWTVRHTLSAVGVAAVIAAFGGVA
                     IYAATSGSSNAMGPGTHGPGTGGFGPGGPGPGGPDGQDPHGLPEMGGPAPLHGEFVVA
                     GENGGYSTELVQTGVLTAVSDTSITARSEDGFTQTYTLAPDSRTANAQLAVDDTVTIH
                     ATKVNGAATATTVAEGSEVGPGGPPAR"
     gene            complement(3326..4303)
                     /locus_tag="VF874_00900"
     CDS             complement(3326..4303)
                     /locus_tag="VF874_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX9174695.1"
                     /translation="MVGWLDRLQRRSRAAGFVIAVIYKYVDDQGGYLAALITYYAFVS
                     LFPLLLLFTTVLGVVLAGHSELQNQVMNSALSQFPVIGDQLGDPQRLSGGALAIVVGI
                     AGALYGGLGVGQAVQNAMDTVWAVPRHVRADPIRSRVRSLILLLLLGSALTGATLLSA
                     LGRESAALGPAGKAGVVVAAVAINAAVCLIAFRVTTARGLTFRHIAPGAVTAAVIWQL
                     LQWFGAAYVTHVVKSASMTNSVFALVLGLLAFLYLTSVALVLSAEINVVRVDHLYPRA
                     LLAPFTDDVALTAGDRKTYTGQAKAQQAKGFEHIDVTFHPTQSESEKQR"
     gene            4450..5031
                     /locus_tag="VF874_00905"
     CDS             4450..5031
                     /locus_tag="VF874_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX9174696.1"
                     /translation="MATQSRAAIHHGPGSPLTFDITRLPRRPGFMQQVRETVPSPSRI
                     GLDLVAIAEGAPLDLDLKFESVSEGVLVTGTVSAPTSGECARCLTATSGEVEVHLTEL
                     FAYPDSTTEATTEADEVGHVVDGRIDLEQQLVDAVGLELPLSPVCRDDCPGLCAECGV
                     ALAEAGPGHHHDQIDPRWAKLSGMYSDESPERP"
     gene            5028..5720
                     /gene="rnc"
                     /locus_tag="VF874_00910"
     CDS             5028..5720
                     /gene="rnc"
                     /locus_tag="VF874_00910"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX9174697.1"
                     /translation="MSGSRDALIAALGVELPEDLLTLALTHRSYAYEHGGLPTNERLE
                     FLGDAVLGLTITDELFHRHPDRPEGDLAKLRASIVNTHALADVARGLTDSGLGAYLLL
                     GRGEENTGGSGKASILADTMESLLGAVYVHHGIDTAREVILRLFGTLLDTAPTLGAGL
                     DWKTSLQELTAARGLGVPSYVVTSTGPDHDKQFTATVIVMDTEYGAGVGRSKKEAEQQ
                     AAAAAWNALDKA"
     gene            5725..6579
                     /gene="mutM"
                     /locus_tag="VF874_00915"
     CDS             5725..6579
                     /gene="mutM"
                     /locus_tag="VF874_00915"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEX9174698.1"
                     /translation="MPELPEVETVRRGLAAHVAGRTITAVRVHHPRAVRRHEEGAADL
                     TARLLGQRIVDTGRRGKYLWLNMSDGTALVVHLGMSGQMLLGPVPKTDHVRIAALLDD
                     GTTLSFVDQRTFGGWMLAELVEVDSSVVPVPVAHLARDPLDPDFDADAVVTVLRRKHS
                     EIKRQLLDQTVVSGIGNIYADEALWRAKVNGARIASSLTRRQLREVLDCAAEVMRDAL
                     GQGGTSFDSLYVNVNGESGYFDRSLDAYGREGLPCRRCGSVIRREKFMNRSSFYCPRC
                     QPRRRVRL"
     gene            complement(6602..8014)
                     /locus_tag="VF874_00920"
     CDS             complement(6602..8014)
                     /locus_tag="VF874_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9174699.1"
                     /translation="MDRLDPLDAAFVEMEPRIQPQHVAAVLILSPPDDAGPDYVDDLY
                     RAALASTAPIDPRLRRYPHRGRDTRGMWVWRDAENLDISEHVKRATLPPGADREAFWL
                     LVSVLHAQGLDRSRPMWSLHLIDGLDDGRFAFYIKVHHAVMDGVAGLHMITEALSPDP
                     TRRSMPPLYGAQHDEPAPEATSAGGRIPNPFTMLRSLLSGATSSAALVEKVVTGQVSN
                     VVAGLTSDTTVAPLAAPYTRFNGPLGDARSIAAGSWSKNRISAIQDKAAVTGNDVVVA
                     VIAGMVRSWLLDHGELPEQSLVALCPITVRAREHGPGDEHGNMFGAWLCPLGTDLEDP
                     AERLDLIHRSMAEGKQQVASRGSGASLLLLMPTQALTVLLPKVRFAPKVRTGYNVPIT
                     NVPGPKTEMYWNGAHVEELCPVATIFNGLTLTVTVCSYADRVSFSYLTDPDVVPDIGS
                     VLGLTERALTELEAAVGVSR"
     gene            8148..8696
                     /locus_tag="VF874_00925"
     CDS             8148..8696
                     /locus_tag="VF874_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX9174700.1"
                     /translation="MPHSFDLSAESPASVDQVHAAFGDEDYWRARLVALGAGPRVATL
                     DSLIVDAAGTVIVSATFCLVGDRLPKLVSQLGRGDLVMMHTETWSPIGDNQVRGEVRV
                     AMRGSPVSALGVALLAPLGSGSRLKYSATVKVKVPLVGGKIERFMSGRLAEGIMDIQR
                     FTTAWIAENPDGQGVTATRRMR"
     gene            8713..8985
                     /locus_tag="VF874_00930"
     CDS             8713..8985
                     /locus_tag="VF874_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174701.1"
                     /translation="MRRTVIVIEPGSFQSAMSHDIPERLAASLPPGSPFKPIVEFTER
                     TSAREERHARDPKILARAVYRAVQAALPGQRRDTKQDSERLAEDGC"
     gene            9031..10575
                     /locus_tag="VF874_00935"
     CDS             9031..10575
                     /locus_tag="VF874_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEX9174702.1"
                     /translation="MTTDNPVIETSYGPVRGTDDGRVKAWKGIRYAAPPVDDLRFRAP
                     EPPEPWTEIAEATAFGPACPQPPLPNIPLDLGAPQGEDCLSLNIWASSDTEPGARKPV
                     MVWLHGGAYVLGSSGQALYDGRRLAGSGDVVIVTLNYRLGALGFLDLSSFNTPRRRFD
                     SNIGLRDVLAALHWVRDNVAAFGGDPQRVTLFGESAGAGIVTTLLATPSANGLFANAI
                     AQSSPATSVYDRGRGRRVAEHILDKLGIDPSDADRLDRAPIAAILAASKEVFDEVPVR
                     NPGTLAFVPIVDGDLLTDYPVKLAQEGRTHPVPLIIGTNRHEAALFRLMRSPLMPITP
                     RAITSMFTEIAAEQPDLQLPTQKQIAVAYSRVRGKARYLSIASDVGFRMPSVWLAEGH
                     RAVAPVYLYRFDYSTPLLKLLLVKAAHATEMPYVWGNLGGPKDPMLKLGGAKTAKAVS
                     KRVRTRWINFATQAKPIGAPGEPDWTPYQQTDRACLIIDKRDAVVYDIDAHIRAAWGD
                     EVVSLR"
     gene            10671..11084
                     /locus_tag="VF874_00940"
     CDS             10671..11084
                     /locus_tag="VF874_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9174703.1"
                     /translation="MTELWVERTGVRRYTGRSSRGAEVLVGNEDVDGVFTPGELLKIA
                     LAACSGMATDAPLARRLGDDHKATVRVSGPPDREHEIYPLLEEALEVDLSGLSDDEAA
                     RLLTVVNRAVEQACTVGRTLKEGTTVAFEVKRSGC"
     gene            11074..11352
                     /locus_tag="VF874_00945"
     CDS             11074..11352
                     /locus_tag="VF874_00945"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX9174704.1"
                     /translation="MAADVRLTAWVHGYVQGVGFRWWTRSRARELGLTGYASNRPDGR
                     VQVVVQGPRENCERLLDLLQGGNTPGTVNNVVADWSEATEPINGFSER"
     gene            11364..>14665
                     /gene="smc"
                     /locus_tag="VF874_00950"
     CDS             11364..>14665
                     /gene="smc"
                     /locus_tag="VF874_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_040963272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX9174705.1"
                     /translation="MHLKSLTLKGFKSFASPTTLRFEPGITCVVGPNGSGKSNVVDAL
                     SWVMGEQGAKTLRGGKMEDVIFAGTSSRAPLGRAEVTVTIDNSDNALPIEYSEVSITR
                     RMFRDGASEYEINGSSCRLMDIQELLSDSGIGREMHVIVGQGKLSEILESRPEDRRAF
                     IEEAAGVLKHRKRKEKAVRKLEAMSANLARLTDLTIELRRQLKPLGRQAEVARRAQTI
                     QADLRDARLRLAADDLVARRNDFAGVGDAEAALRREHDEASALLETASTEFTGHEEAV
                     ATLTERAERAQQTWFRLSALAERVSATVRIATERAQHLETEPVQTSGIDPDMLDAEAD
                     EVAATETRLVADLDDASARLEVARARMAEAERLATEAERAHLAAVRAEADRREGLARL
                     AGRVDTMRTRVESIDDAVARLTEAIDEAAARAQQAQAEFETVQTRVGALDEGEVGLDE
                     HHDRSVAALRLADERVAELQAAERSAERQVASLQARIDALSVGLDRKDGAAWITENFS
                     GAGFFGSLASLVKVRHGYEAAVATVLGAAADAIAAEDVKAASSAVAALKDSDGGRAAI
                     VLGGWPAPADPRPAPLPPDALWAVDLVEAPERLRGAVAAMLSGVAVVGSLDTALDLVA
                     ARPQLRAVTIDGDLVGAGWVSGGSDRRPSTLEITSEIENARAELTRADTQAHELAAAL
                     AGALAEQTARQDAAEQALAALNESDATISSAYEQLGRLGQDARATEEEWRRLTEQRSE
                     LEGTRVRTVGELTELEQRLTNAEQTQESVADDSAERQSTVAAAEAARGVEIEARLAVR
                     TAEERVNAVRGRADSLRRAAAAEREARVRAQRAREARRHAAAVAAAVAESGRQVALRL
                     AEAIAAASRTRDALIAERTQRANARSEARATVSALTTKIGTLTEALHRDEVAKAQAAL
                     RIEQLEEHILEQFGMATEDLIAEYGPDVPLPPSELALAEYERARERGEQVSAPAPMPF
                     DRPTQERRAKKAERELAELGRVNPLALEEFAALEERYNFLSTQLEDVKAARKDLLSVV
                     ADVDARIVSVFSEAYADVEREFRAVFEALFPGGEGRLLLTNPDDMLTTGIEVEARPPG
                     KKIKRLSLLSGG"
BASE COUNT         2436 a         4921 c         5098 g         2210 t
ORIGIN      
        1 catcacccac agcgctgcgg gcgtcttgtc caccgtgatc gcgttcgcgg cgtcgctgga
       61 gccgaacagg aatgcggtcc tgctctgcga accagcttgc accgcagcgg aatagaagcc
      121 gttggcccaa ccgctcgcgg acaggttcca caggtagagg cacgcggtca cgaccagcag
      181 ccctgtgaac gcggcccggt gaacgggaaa acgttgcggc cgttgacgga tgacgggcgc
      241 gtcgtggacg gtccgcggcg gtgagcgtca tcagtccatt ctggggttgg ttgctaggcg
      301 ccggatttgt cgtcgctggg ggctggctgt gaagccgcca gccggacggt gaacgcggtg
      361 tcgccgggca cgctgcgcac gtcgatgctg ccgtgatgcg ccttgaccac cgcggacacg
      421 atcgccagtc ccagccccgt gctgccgtcg cggcgcgacc gggatgtgtc accgcgggcg
      481 aaacgctcga aaatctccgg ccgtaccgcc gcgggaatgc cagggccgtc gtcggcgacc
      541 gtcaacaccg cgccgcccgc gccggcgctg gccaacgacg tcgtcactga tgtacctgct
      601 ggggtgtggg tgcgcgcgtt ggcgagaagg ttcaccagca cctgctgcag gcgctggccg
      661 tcaccagtga ccaccacggg ctcatcgggc aggtccagcg accatcgatg ctccggtccg
      721 gcgacatgcg cgtctgcgac cgcgtccacg gccagctgcg acaggtccac gcgttcccgc
      781 tgcagcggac gtccggtgtc cagtcgtgcc agcagcagca tgtcttcgac gagctgcgtc
      841 atgcgcgcgg cctccgactc gacgcggccc atcgcatgcg cgacatcggc gggcacgtca
      901 ccacgacggc gctgcgccag ttcggtatag ccgcggatcg ccgagagcgg agttcgaagc
      961 tcgtggctcg cgtcggcgac gaactgccgc acccgcgtct cgctggcatg ccgggcggcc
     1021 aacgcgtccg agatccgttc gagcatgcgg ttcagcgctg ccgcgagctg ccccacctcg
     1081 gtgtgcgcgg ccgccgggtc gacggcaagg acatgcgtcg gcagcgtcac ctccccgcga
     1141 tcgagctcga ggttggcgac gtcgcgggcg gttgccgcca cccgcgacac cggggcgagc
     1201 tggcgtcgga tgatcacgat tcctgccgcg atggccgcga tgagcgcgat gcccgcgacg
     1261 atgccgaaga tcgttcccac cgacagcagc gtgttgtcga cctcggccat cggcagaccg
     1321 gaaacgaccg tgtcgccgct ccgagagtgg cgtgcgatca cgcggtaccg gccgaggccg
     1381 tcgaggttca cggtgaccgg ccggccgtca ggcgtggtgc gtgacagctg cgcggccgcg
     1441 gcattgctga tgctttcccg ggtgccgttt tgggtgatga cgcccgcgtc gagaaccgtg
     1501 ccgtcgcgca ttaccgcgcc gaccgtgcca gtcgcttggc cgggcgcgtc gaggaacatc
     1561 ggcccgggcc cgctgtggcg gtcgaacgcc ggcggacgcc gcgggtatcc cggtggcgga
     1621 gacagcggca gcccgagatc gaagatggcc tccgatcggc ggccggcgtc gacgacccgc
     1681 tcgtcgagct ggcgcatcag aaaacggtgc agcgcgagtt cggttcccac gccgatgccc
     1741 acgcagacga cggcgagcag ggtcaccaca gtgacgagca ggcgcgtgcg caacgaccag
     1801 gcccgcgggc gcagcctcgc ggaccggcgt gcgtcagtcg gcaggtttga ggacatagcc
     1861 tgcgccccgc agcgtgtgaa tcatcggtgc gcgaccgttg tcgatcttct tgcgcaagta
     1921 cgacacgtac agctcgacga tgttggaccg gccgccgaag tcgtaactcc acacccggtc
     1981 gaggatctgg gccttgctca acacttttcg ggcattgcgc atcatgaacc gcaacagctc
     2041 gaattccgtt gtggtgagcc cgatcgcgtc gccgccgcga gtcacctcgt ggctgtcctc
     2101 gtcaagaaca aggtcgccga cgacgatcct cgcgccgctg gtttcggtgg tgaccccggt
     2161 gcggcgcagc agtgcgcgca gccgcagcac cacctcctcg agcgaaaacg gcttggtgac
     2221 gtagtcgtcg ccgcccgcgg tcaacccggc gatgcggtct tccaccgcgt ccttggcggt
     2281 gagcagcagc agcggcagcc cggggagctg ctcgcgcagc ttgcgcagca cgtccagccc
     2341 gctcatgtcg ggcagcatca cgtcgagtac caccacatcg ggcgcgtcct tgcgcgccgc
     2401 cgcgatcgcc gaggcgccgt cacctgccgt cgacacggtc cagccctcgt accgcagtgc
     2461 catcgtcacc agttcggcca gcacctgctc gtcgtcgacg accaggacgt ggatggggct
     2521 gccgtcggcg cgacgcatga cgacccgacc caggtcgttg acggctgagc gttgagcgtt
     2581 ggacaccacg acaatcatct gtgtgcggga ttggtcttgg ctgtgccgat cctatgcgcg
     2641 cgctgtgaat cactcgtagg catatcagac gctgaacccg cgcacagcct gcacccatgt
     2701 tccggcgaca cagtgaaagc cgacaagtcg acctgaaagg gcttaccgaa tgagttctga
     2761 cacgaacacc gccgacgaga ccgtctgggg tgcgccacag ccgggcagga agtggacggt
     2821 ccggcacacc ctgtcggcgg tcggcgtcgc cgcagtgatc gccgcgttcg gcggtgtggc
     2881 catttacgcc gcgaccagtg gcagctccaa cgcgatgggc cccgggacgc acggccccgg
     2941 caccggcggg ttcggccccg gtggtcccgg ccccggcggc cccgacggcc aagacccgca
     3001 cgggcttccc gaaatgggcg gccctgcccc gctgcacggc gagttcgtgg tggccggcga
     3061 aaacggcggc tacagcaccg aactcgtcca aacgggggtc ctcaccgcgg tgtccgacac
     3121 gtcgatcacc gccaggagtg aggacgggtt cacgcagacc tacacgctcg ctccggacag
     3181 ccgcacggcg aatgcgcaac tggcagttga cgatacggtc acaatccacg cgacgaaagt
     3241 caacggggcc gcgaccgcga cgacggtggc cgagggcagt gaggtcgggc ccggtggacc
     3301 gccggctcgc tgagccgtcc agcagtcaac gttgcttctc ggactcagat tgcgtcggat
     3361 ggaacgtcac gtcgatgtgc tcaaatccct tggcctgctg ggcttttgct tgaccggtat
     3421 aggtcttgcg atctccggcc gtcaacgcca cgtcgtcggt gaacggggcc agcaatgccc
     3481 gaggatacag gtggtccacc cgcacgacgt tgatctcggc ggacaagacc agggcaacgg
     3541 aggtcaggta caagaacgcg agcaacccca gcaccaacgc gaacacgctg ttggtcatgc
     3601 tcgccgactt gacgacatgc gtcacgtacg cggccccgaa ccactgcaac agctgccaga
     3661 tcacggccgc ggtgaccgcg ccgggcgcga tgtgccggaa tgtcagcccg cgcgcggtgg
     3721 tcacccggaa cgcgatcaga cacaccgcgg cgttgatcgc gaccgctgcg accaccacgc
     3781 cggccttgcc ggccgggccg agtgccgccg actcgcgacc cagtgccgac aacagcgtgg
     3841 cgcccgtgag cgccgagccg agcagaagca gcaggatcag gctgcggacg cgggagcgga
     3901 tcgggtccgc acgcacgtgg cgcggcaccg cccacacggt gtccatcgcg ttctgcactg
     3961 cttggcccac tcccaggccg ccgtacaacg ctccggcgat gccgacgacg atcgcgagcg
     4021 cgccgccgct gagccgttgc ggatccccga gttgatcacc gatcaccggg aactggctga
     4081 gcgccgagtt catcacctgg ttctgcaact cggagtggcc ggccagcacc accccgagca
     4141 cggtcgtgaa cagcagcaga agtggaaaca gggacacgaa cgcgtaatag gtaatcagcg
     4201 cggccagata gccgccctgg tcgtcgacgt acttgtagat caccgcgatg acgaacccgg
     4261 ccgcgcggct tcgccgctgt agccggtcca gccaacccac catggcgaaa ctcccctcgg
     4321 tgccttgatg ctcctcgcgt ccgcgtgtgc ggacgtcccg cacacgacgc aggtcatacc
     4381 cgctttctcg gtgggcaatc ccggatgggg cgtcggccct tgccgacgat gggtgcctac
     4441 gatggcgtca tggccacgca gtcgcgcgcg gcaatccatc acggccccgg atcgccgctc
     4501 acgttcgaca tcacccggct tccccgccgt cccggcttca tgcagcaggt gcgggagacc
     4561 gtgccgagcc cgtcgcgcat cgggctcgac ctggtcgcga tcgccgaggg ggcgccactg
     4621 gacctggact tgaagttcga gtcggtgtcc gaaggcgtgc tggtgaccgg cacggtgagc
     4681 gccccgacat cgggggagtg cgcgcgctgc ctgaccgcga cgagcggtga ggtcgaagtc
     4741 cacctcacgg agctgttcgc gtatccggac agcaccaccg aggcgaccac cgaagcggac
     4801 gaggtcggcc acgtcgtgga cggcaggatc gacctggagc agcagctggt cgacgccgtc
     4861 ggattggagt tgccgctgtc accggtgtgc cgcgacgatt gtcccggcct gtgcgccgaa
     4921 tgcggtgtgg ccttggctga ggccggaccc gggcaccacc atgatcagat cgatccgaga
     4981 tgggcgaaac tgtccggcat gtactccgat gagtcgccgg aacgcccgtg agcggatcgc
     5041 gtgacgcgct gatcgcggca ttgggcgtcg agctgcccga agacctgctc acgctggcgt
     5101 tgacccaccg cagctacgcc tacgagcacg gcggacttcc gaccaacgag cgcctcgagt
     5161 tcctcggcga cgcggtgctc gggctgacga tcaccgatga gctgttccac cgccaccccg
     5221 accggcccga aggcgacttg gccaagctgc gggcgagcat cgtcaacacc cacgcgctgg
     5281 ccgacgtggc ccgcggcctc accgacagcg ggctgggcgc ctacctgcta ctcggccggg
     5341 gtgaagagaa caccggcggc tcgggcaagg ccagcatcct cgccgacacc atggagtctt
     5401 tgctgggggc ggtgtacgtg caccacggaa tcgataccgc gcgcgaggtg attctgcggc
     5461 tgttcggcac gctgctcgac accgcgccga cgctgggcgc cggcctggac tggaagacca
     5521 gcctgcagga actgaccgcc gcgcgggggc tcggtgtgcc gtcgtacgtc gtcacatcca
     5581 ccggccccga ccatgacaag cagttcaccg caacggtgat cgtgatggac accgaatacg
     5641 gcgccggtgt cgggcgatcc aagaaagagg ccgagcagca ggccgctgct gcggcgtgga
     5701 acgccttgga caaggcctga gccagtgcct gagctgccgg aagtcgagac cgtccggcgc
     5761 ggactggccg cccacgtcgc gggccggacc atcaccgccg ttcgggtgca ccatccccgg
     5821 gctgtgcgcc ggcacgagga aggggccgcc gacctgaccg cgcgcctgct cgggcagcgc
     5881 atcgtcgaca ccggccggcg cggaaagtac ctgtggctca acatgtctga cggcactgcg
     5941 cttgtcgtcc acctggggat gagcggccag atgctgctgg ggccggttcc gaagacggac
     6001 cacgtgcgga tcgctgcgct gctcgacgac ggcaccacac tgagcttcgt cgaccagcgc
     6061 acgttcggcg gatggatgct ggccgagttg gtggaagtcg attccagcgt ggtccccgtc
     6121 ccggtcgcgc acctggcgcg agacccgctg gatccggact tcgacgccga tgccgtcgtt
     6181 acggtgttgc gccgcaagca ctctgagatc aaacggcaac tcctcgacca gacggtggtg
     6241 tcgggcatcg gcaacatcta tgccgacgaa gcgctgtggc gcgcgaaggt caacggcgct
     6301 cgcatagcgt cgtcgctgac ccgtcgccaa ttacgggagg tgctcgactg cgccgccgag
     6361 gtgatgcgcg acgcactggg ccagggcggc acgtcgttcg actcgctgta tgtgaacgtc
     6421 aacggtgagt cgggctactt cgaccgctcg cttgacgcgt acggccgtga gggactgccg
     6481 tgccgccgct gcgggtcggt gattcgccgc gagaagttca tgaaccgctc gtcgttctac
     6541 tgcccacggt gccagccgcg gcgccgtgtt cgcctttgac cacggctccg gggccgaact
     6601 gtcagcgcga cactcccact gccgcttcga gttcggtcag ggcccgttcg gtgagaccta
     6661 acaccgagcc gatgtcgggc accacgtcgg ggtccgtcag gtaactgaag ctgacgcgat
     6721 cggcgtacga gcacacggtc acagtcagtg tcaggccgtt gaagatcgtc gccaccggac
     6781 agagctcctc gacatgtgca ccgttccaat acatttcggt cttcggccct ggaacattcg
     6841 taatcgggac gttgtatccg gtgcggacct tcggcgcaaa ccgcactttc ggcagcaaca
     6901 cagtcagtgc ttgcgtcggc atgagcagta acagcgacgc tcccgagccg cgactggcca
     6961 cctgctgctt gccttccgcc atcgaccggt ggatcagatc aagccgttca gcgggatcct
     7021 cgagatcggt gcccagcggg cacagccatg cgccgaacat gttgccgtgc tcgtcacctg
     7081 gaccgtgctc gcgagcccgc acggtgatcg ggcagagcgc caccagcgac tgctcgggaa
     7141 gttcgccatg gtcgagcagc cagctccgca ccatcccggc gatcaccgcg acgacgacgt
     7201 cgttgccggt cactgccgcc ttgtcctgta tggcgctgat gcggttcttc gaccagctgc
     7261 cggccgcgat gctgcgcgcg tcgccgagtg gaccgttgaa ccgggtgtaa ggagctgcca
     7321 gaggcgcgac ggtggtgtct gaagtcaggc cggcgaccac gttggacacc tggccggtga
     7381 cgactttctc gacgagcgcg gcgctcgacg tggccccgct taacagcgac cgcagcatcg
     7441 tgaacgggtt gggtatccgc ccgcccgccg acgtcgcttc cggggcgggt tcgtcatgct
     7501 gggctccgta gaggggcggc atggaccggc gtgtcgggtc ggggctcaac gcctcggtga
     7561 tcatgtgcag accggcgacg ccgtccatga ctgcgtggtg caccttgatg taaaaggcaa
     7621 acctgccgtc atcgagaccg tcgatgagat gaagcgacca catcggacgc gaccggtcga
     7681 gcccctgggc gtgcagcacg ctgacgagca gccagaacgc ctctcggtcg gcaccgggcg
     7741 gcaacgtggc gcgcttgacg tgttcgctga tgtcgaggtt ttcggcatca cgccagaccc
     7801 acatgccgcg ggtgtccctg ccgcgatggg gatagcggcg aagccgcgga tcgatcggag
     7861 ccgttgaggc gagcgcagcc cggtacagat cgtcgacgta gtccgggccc gcgtcgtcgg
     7921 gaggcgacaa gatcagcacg gctgccacgt gctgcggctg gatcctcggc tccatctcca
     7981 cgaacgccgc atccagcgga tccaaccggt ccatagacgc agactccaat cagcgcaggg
     8041 ggctggggtc accataggcc gcgtaagcga cgtcggcgtc aaaatcggtc agcccaactt
     8101 agagcaacag cctccgtgcc acggccggcg gcgtacgctg cctcgctatg ccacactcgt
     8161 tcgacttgtc ggcagagtcg ccggccagcg tcgatcaagt ccatgcggcg ttcggcgacg
     8221 aggattactg gcgggcgagg ctggtcgcct tgggcgctgg cccccgcgtt gccacgctcg
     8281 actcgttgat cgtcgatgct gccggcacgg taatcgtttc cgcgacgttc tgccttgttg
     8341 gtgaccggct acccaagctg gtgagtcagc tgggccgcgg tgacctggtg atgatgcata
     8401 ccgaaacatg gagtccgatc ggcgacaacc aggtgcgtgg ggaggtgcgc gtcgcgatgc
     8461 gcgggtcgcc ggtatccgcg ctgggcgtgg cgttgctggc gccgctgggg agcggctcgc
     8521 ggctgaaata cagcgcgacg gtgaaggtca aagtcccgtt ggtgggcggc aagatcgagc
     8581 gcttcatgag cggtcgactc gccgaaggaa tcatggacat ccagcgcttc accacggcgt
     8641 ggatcgctga aaaccctgat ggccaagggg tgacggcgac acgacggatg cggtgagaga
     8701 ggtttgggct cgatgagacg cacggtcatc gtcatcgaac ccgggagctt ccagagcgcg
     8761 atgagccacg acatccccga gcggctggcc gcatccctcc cacccggatc accgttcaag
     8821 ccgatcgtcg agttcaccga acggaccagc gcccgggaag aaagacacgc gcgcgatccg
     8881 aagattctgg cgcgcgcggt gtatcgcgcg gtccaagccg cgctacctgg tcaacggagg
     8941 gatacaaagc aggattctga acgtcttgcc gaggacggct gctgaccggc tcgtcaagct
     9001 ggtggtgcgc tgacggctag cgtgagacgt atgacaaccg acaaccctgt gatcgagacg
     9061 agttacggcc ctgtccgtgg caccgacgac ggccgcgtca aggcgtggaa gggcattcgc
     9121 tacgccgcgc cgcccgtgga cgacctgcgc ttccgggcgc ccgaaccgcc cgaaccgtgg
     9181 accgagatcg ccgaggccac cgcgttcggt ccggcctgcc cgcaaccgcc gctgccgaac
     9241 atcccgctcg acttgggcgc cccgcagggc gaggactgcc tgagcttgaa catctgggca
     9301 tcgtcggaca ccgagccggg cgccagaaaa cccgtgatgg tgtggctgca cggtggcgcc
     9361 tatgtcctcg ggtccagcgg ccaggcgctc tatgacggtc gccggctggc cggcagcggc
     9421 gacgtcgtca tcgtgacact caactaccgg ctcggtgcgc tgggttttct cgatctgtcg
     9481 tcgttcaaca cgccacggcg acgtttcgat tcgaacatcg ggctgcgcga cgtgctggcc
     9541 gccttgcact gggtgcgtga caacgttgcg gcgttcgggg gagatccgca gcgggtcacg
     9601 ctgttcggcg aatccgccgg cgccggtatc gtcaccacgc tgctcgccac accgtcggcc
     9661 aacggcctgt tcgcgaacgc gatcgcccag agctcaccgg ccacgtcggt ctacgaccgt
     9721 ggcaggggtc ggcgcgtcgc agagcacatc ctcgacaagt taggcatcga cccctccgac
     9781 gcggaccggc tggaccgcgc gccgatcgcc gcgatccttg ccgcctcgaa ggaagtgttc
     9841 gacgaggtgc ccgtacgcaa cccgggcacg ctggcgttcg tcccgatcgt cgacggcgac
     9901 ctgctcaccg actacccggt caagctggca caagagggtc gcacgcaccc ggtcccgttg
     9961 atcatcggca cgaacaggca tgaagcagct ctcttccggt tgatgagatc gccgctgatg
    10021 ccgatcaccc cccgcgcgat cacgtcgatg ttcaccgaga tcgccgccga gcagcccgac
    10081 ttgcagctgc cgacccagaa gcagatcgcg gtggcgtatt cgcgagtgcg gggcaaagcc
    10141 agatatctca gcatcgcgag cgacgtcggc ttccggatgc cgtcggtgtg gctggccgag
    10201 gggcaccgtg cggtggcccc ggtgtacctg taccggttcg actactcgac tccgttgctg
    10261 aagctgctgc tggtcaaggc cgcccatgcc accgaaatgc cttacgtgtg gggcaatctc
    10321 ggcggaccca aggatcccat gttgaagctc ggcggtgcga agaccgcgaa ggcggtgtcc
    10381 aagagggtcc ggacccggtg gatcaacttc gcgacgcagg ccaagcccat cggtgcaccc
    10441 ggcgaaccgg actggacgcc ctaccagcag accgaccgcg cctgcctgat catcgacaag
    10501 cgcgacgccg tcgtgtacga catcgacgcc cacatccggg ccgcgtgggg cgacgaggtg
    10561 gtcagtttgc ggtagcggca accgcggatt cgccgaaatt gcgttccacg tggcccgtac
    10621 tcgcatttct ccgcgctggt ggcagtctcg gcgaaaggta gaactatcgc atgacagaac
    10681 tctgggtgga gcgcaccggc gtgcgtcgct acacgggccg cagctcgcgc ggcgctgaag
    10741 tgctggtggg caatgaggac gtcgacggtg tcttcacgcc cggcgagctg ctgaagatcg
    10801 cattagccgc gtgcagcgga atggccacgg acgcgccgtt ggcccgtcgg ctcggcgacg
    10861 accacaaggc caccgtgcgc gtctccggac cgcccgaccg tgagcacgag atctatccgc
    10921 tgctggagga agcccttgag gtggatctgt ccggtctgag cgacgacgag gcggcccggc
    10981 tattgacggt cgtgaaccgg gccgtcgagc aggcgtgcac ggtcggccgc acgctcaaag
    11041 aggggaccac cgtggcattc gaggtgaaac gcagtggctg ctgatgtccg gctgaccgca
    11101 tgggtgcacg gctacgtgca gggcgtgggc tttcggtggt ggacccgctc gcgggcgcgg
    11161 gagctgggtt tgaccggcta cgcgtcgaac aggccggacg gtcgggtcca ggtcgtggtg
    11221 cagggcccgc gcgaaaactg cgagcgcctg ctcgaccttc tccagggcgg caacacgccg
    11281 ggcaccgtca acaacgtggt cgcggactgg tcggaggcga cagagcccat caacggcttc
    11341 agcgagcgat agtctcacac ctcgtgcacc tcaagagtct gacgctgaag ggcttcaagt
    11401 ccttcgcctc gccgacgact ctgcgtttcg agccgggcat cacctgtgtc gttggcccca
    11461 acgggtcggg caagtccaac gtcgtggatg cgctgagctg ggtgatgggc gagcagggcg
    11521 ccaagacgct gcgcggcggc aagatggagg acgtcatttt cgccggtacg tcgtcacgcg
    11581 ctccgctggg ccgcgccgag gtgacggtca cgattgacaa ttccgacaac gcgctgccga
    11641 tcgagtactc cgaggtgtcg atcacccgtc ggatgttccg cgacggcgcc agcgagtacg
    11701 aaatcaacgg cagctcttgc cgtttgatgg acatccagga gttgctgtcc gattcgggca
    11761 tcggccgcga gatgcatgtg atcgtcggcc agggcaagtt gtcggagatc ttggagtccc
    11821 gcccggagga ccgccgggcg ttcatcgagg aagccgccgg cgtgctcaag caccgcaagc
    11881 gcaaggagaa ggcggtccgc aagctcgagg cgatgtccgc caacctggcg cggctcaccg
    11941 atctcaccat cgagctgcgg cggcagctca agccgctcgg tcgccaagcc gaggtggccc
    12001 gccgcgccca gacgatccag gccgacctgc gagacgcccg gctgcgtctg gccgccgacg
    12061 atctggtggc acggcgcaac gacttcgccg gcgtcgggga tgcggaggct gcgcttcgcc
    12121 gagagcacga cgaggcgtcc gcgctgctgg agacggcgtc gacggagttc accggccacg
    12181 aagaagcggt ggccacgctg accgagcgtg ccgaacgcgc gcagcagacc tggtttcggt
    12241 tgtccgcatt ggccgaacgg gtcagcgcga ccgtgcggat cgcgaccgaa cgcgcgcaac
    12301 acctcgagac cgagccggtg cagacctctg gcatcgaccc cgacatgctg gacgcggagg
    12361 ccgacgaggt cgctgcgacc gagacgcggc tggtcgcgga cctcgacgac gcgagcgcgc
    12421 ggttggaggt cgcccgcgcg cgaatggcgg aggctgagcg cctcgccacc gaagccgagc
    12481 gggcgcactt ggccgccgtg cgcgccgaag cggaccggcg ggagggcctc gcccggctcg
    12541 ccggccgggt cgacacaatg cgcacccgcg tcgagtcgat cgacgacgcg gtggcgcgtc
    12601 tcaccgaggc gatcgacgag gccgccgccc gcgcgcagca ggcccaggct gagttcgaga
    12661 ccgtccagac ccgcgtcggt gccctcgacg aaggcgaggt cggactcgac gaacaccacg
    12721 accggtcggt ggcggcgttg cgcctggccg acgaacgggt agctgaactg caggccgccg
    12781 agcgaagtgc cgaacgccag gtcgcttcgc tgcaggctcg catcgacgcc ctctcggtgg
    12841 ggctcgaccg taaggacggc gccgcctgga tcaccgaaaa cttcagtggt gcaggatttt
    12901 tcggctcgtt ggcaagtctg gtcaaggtgc gtcacggtta cgaggcggcg gtggcgactg
    12961 tgctcggggc ggccgccgac gcaatcgccg cggaggatgt gaaggccgct tcgagtgcgg
    13021 tcgcggcgct gaaggactcc gacggcggcc gggcggcgat cgtgctcggc ggctggcccg
    13081 cgcccgccga tccgcggccg gccccgttgc cgcccgacgc gctgtgggcg gtcgacctgg
    13141 tggaggcgcc ggaacggctg cgcggcgctg tcgcggcgat gctctccggc gtcgcggtcg
    13201 tcggatccct ggacaccgcc cttgacctgg tggcagcacg tccgcagctg cgcgcggtca
    13261 ccatcgacgg cgatctggtc ggcgcgggct gggtcagcgg cggctccgac cggcggccca
    13321 gcacgctgga gatcacctcc gaaatcgaga acgcccgcgc cgagctcacg cgggccgaca
    13381 cccaggcgca cgagctggct gcggcgctgg ccggtgcgct tgccgagcag accgcacgcc
    13441 aggacgccgc cgaacaagcg ctggcggcgc tcaacgagtc ggacgccacg atttcgtcgg
    13501 cctacgagca gctcggccgg ctgggtcagg acgcgagggc cacggaggag gagtggcggc
    13561 gcctgaccga gcagcgctca gagctcgagg gcactcgcgt gcggaccgtc ggcgagttga
    13621 ccgagctcga gcagcggctg accaacgccg agcagacgca ggaatccgtc gccgacgact
    13681 ccgccgagcg ccagtcgacg gtggcggcgg ccgaggcggc gcggggagtc gagatcgagg
    13741 cgcggctggc ggtgcggacc gccgaggaac gtgtgaatgc cgttcgggga cgggcagatt
    13801 cgctgcgccg ggccgccgcc gcggaacgcg aggcgcgggt gcgcgcgcag cgggcgaggg
    13861 aggcccgccg ccacgccgca gccgtcgccg ccgcggtggc cgaatccggc cggcaggtgg
    13921 cgctgcggct cgccgaggcg attgcggccg cgtcgagaac ccgcgacgcg ctgatcgccg
    13981 aacgcacgca gcgcgccaac gctaggtcgg aggcgcgcgc gacggtgtcc gcgctgacga
    14041 ccaagatcgg cacgctgacc gaggcgctgc accgcgacga agtggccaag gcgcaggctg
    14101 cgctgcgaat cgagcaactc gaggagcaca tcctcgaaca gttcggcatg gccaccgagg
    14161 acctcatcgc cgagtacggc cccgacgtgc cgctgccgcc gtcggagctc gcgctcgccg
    14221 agtacgagcg ggcgcgggag cgcggcgagc aggtgagcgc accggcgccg atgccgttcg
    14281 accggcccac ccaggagcgg cgagccaaga aggcggaacg cgaactggcc gaactgggtc
    14341 gagtcaatcc actggcgctc gaggagttcg cggcgctgga ggagcgctac aacttcctgt
    14401 ccacccagct ggaggacgtc aaggccgccc gtaaggacct gctcagcgtg gtcgccgacg
    14461 tcgacgcgcg gatcgtgtcc gtgttcagcg aggcctacgc cgacgtcgag cgcgaattcc
    14521 gtgcggtgtt cgaggcgctg ttcccaggcg gcgaaggccg gctgctgctg accaaccccg
    14581 acgacatgct gaccacgggc atagaggtgg aggcccggcc gcccggcaag aagatcaagc
    14641 ggttgtcgct gctgtccggt ggcga
//
LOCUS       DASWBF010000034         7025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_988411,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000034 DASWBF010000000
VERSION     DASWBF010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988411"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..133
                     /gene="rrf"
                     /locus_tag="VF874_00955"
     rRNA            21..133
                     /gene="rrf"
                     /locus_tag="VF874_00955"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(190..693)
                     /locus_tag="VF874_00960"
     CDS             complement(190..693)
                     /locus_tag="VF874_00960"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9174706.1"
                     /translation="MSAIYFRIIDSPVGPLTLAGRDNTLAHLRMTGQTHEPDRAGWVE
                     DDDAFPDAVEQLGTYFAGELSSFDVDLALTGTEFQRRVWAALQTIPYGETRSYGEIAA
                     QIGAPGASRAVGLANGRNPIGIIVPCHRVIGASGSLTGYGGGLDRKRVLLELEKSRKP
                     AGLTLFD"
     gene            complement(690..2183)
                     /locus_tag="VF874_00965"
     CDS             complement(690..2183)
                     /locus_tag="VF874_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX9174707.1"
                     /translation="MHDDFGGCYRAIQSKDTRFDGWFVTAVLTTKIYCRPSCPARSPF
                     ARNVRFYPTAAAAQRAGFRPCKRCRPDASPGSPEWNVRGDVVARAMRLIADGTVDRDG
                     VTGLAAKVGYTPRQLERLLQAEVGAGPLALARAQRTQTARVLIETTDLPFSDVAFAAG
                     FSSIRQFNDTIREVCASTPTELRQKAARRAGPDAAGEGAGTVALRLPTRTPFAFEGLF
                     GHLAASAIAGCEEVRDGAYRRTLRLPWGNAIVSLTPTPDHVRCVLRLHDFRDLSTAIA
                     RCRRLLDLDADPQSVVDFLGTDRDLAPLVVKHPGQRIPRTVDEQELAIRVVIGQQVSM
                     NAARTHCARIVQAHGTLVADPDGGLTHVFPSVDRLASLDPADLAFPKSRQRSVIALAH
                     ALAGGAVTLGVGCDWQRARRQLLDIPGVGPWTTEMIAMRGLGDPDAFPVADNGIRAAA
                     EHVGLPTGIRALNDHSTRWRPWRSYATQHLWATLDHPINHWPLKEKP"
     gene            complement(2233..3861)
                     /locus_tag="VF874_00970"
     CDS             complement(2233..3861)
                     /locus_tag="VF874_00970"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9174708.1"
                     /translation="MVARLSVAQRLGRVLERVTRQSGRLPDTPDFGSWLLGRAEESQT
                     RRRVRIQVILTVFVVTANFIGIGVAVLLVTIAFPVPSIFTEAPWWLTWVVSPAYTLVA
                     LLVGIFWVTRRTVGALRWAIEERPPTREDQRNTFLAPWHLAEMHLVLWGVGTALLTTL
                     YGVVDTAFIPRYLFAVGFSGIVVSTGCYLLTEFALRPVAAQALEAGHPPRRLAAGIMG
                     RIMTVWLLGSAVPVLGIVLATMFALSLRNMSQTQFGIAVLILGIIALVFGFVLMLIVS
                     WITATPVRVVRAALERVEDGDLDSNLVVFDGTELGELQRGFNSMVHGLRERERIRDLF
                     GRHVGREVAAAAEEQELELGGEERHVAVFFVDMIGSTQLVGSKPPSEVVGQLNRFFTV
                     IVEEVDRHRGLLNKFEGDGSLAVFGAPVRLARPEDDALAAARTVARRLCQEVPECPAG
                     LGVAAGKAVAGNVGAYERFEYTVIGDPVNEAARLCELSKSYPQRLLASDAAVEAASES
                     ERRHWRLDDQVVLRGREKPTRLAIPVDQEHVRRP"
     gene            3906..4577
                     /gene="nucS"
                     /locus_tag="VF874_00975"
     CDS             3906..4577
                     /gene="nucS"
                     /locus_tag="VF874_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEX9174709.1"
                     /translation="MRLVIAQCTVDYLGRLTAHLPSARRLLLIKADGSVSVHADDRAY
                     KPLNWMSPPCWLTETTEDSSAVWVVENKTGEQLRITIEEIEHDSTHELGVDPGLMKDG
                     VEAHLQELLAEHVELLGSGYTLVRREYMTPIGPVDLLCRDEQGRSVAVEIKRRGEIDG
                     VEQLTRYLDLLNRDSLLAPVSGVFAAQQIKPQARTLAVDRGIRCITLDYDQMRGLESD
                     EYRLF"
     gene            4600..4881
                     /locus_tag="VF874_00980"
     CDS             4600..4881
                     /locus_tag="VF874_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174710.1"
                     /translation="MRRRSQRMPRHAPLPTARRIETGPDGYDYEVRPVAAALATKSYR
                     CPGCDHEIHSGTAHVVVWPAGAGGVDDRRHWHTACWRHRATRSPTRKWS"
     gene            complement(4886..5356)
                     /gene="mce"
                     /locus_tag="VF874_00985"
     CDS             complement(4886..5356)
                     /gene="mce"
                     /locus_tag="VF874_00985"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX9174711.1"
                     /translation="MTAEQVDARPVLASALVTGIDHIGVAVADLDAAIAWYHDYLGMI
                     VLHEEVNEDQGIREAMLSVRGAPVGSTQVQLMAPISDSSAIAKFLDKRGPGLQQLAYR
                     VSKLDQLTKRLEDQGIRLLYDEPRRGTANSRINFIHPKDAGGVLIELVEPAPES"
     gene            5439..6623
                     /locus_tag="VF874_00990"
     CDS             5439..6623
                     /locus_tag="VF874_00990"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9174712.1"
                     /translation="MTTSVIVAGARTPIGKLMGSLKDFSGSDLGGIAIAGALEKANVP
                     ASLIDYVIMGQVLTAGAGQMPARQAAVAGGIGWDVPALTINKMCLSGIDAIALADQLI
                     RAGEFDVVVAGGQESMTAAPHLLIGSRAGYKYGDVTVLDHMAYDGLHDVFTDQPMGAL
                     TEQRNDVDQFTRAEQDSYAAGSHQKAAAAWKDGVFADEVVPVKIPQRKGGDPIEFGED
                     ESIRANTTAQSLSGLKPAFRKDGTITAGSASQISDGAAAVVVMNKQKAEELGLSWLVE
                     IGAHGVVAGPDSTLQSQPANAIKKAVAREGISVDQLDVIEINEAFAAVALASIRELGV
                     DAEKVNVNGGAIAVGHPIGMSGARITLHAALELARRGSGYGVAALCGAGGQGDALILR
                     AG"
     gene            6750..>7025
                     /locus_tag="VF874_00995"
     CDS             6750..>7025
                     /locus_tag="VF874_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HEX9174713.1"
                     /translation="MTRPRPPVAPAMAGAVDLSALKQRATQSSDAGRAAGAQAGVEIT
                     ETNFEAEVLARSNEVPVVVVLWSPRSDASIQLADALAGLAATDAGTWS"
BASE COUNT         1255 a         2305 c         2340 g         1125 t
ORIGIN      
        1 aaacaacacc taaatacagt tacggcgaac atagcgacag ggaaacgccc ggacccatcc
       61 cgaacccgga agctaagcct gccagcgccg atgatactac ccaaccgggt ggaaaagtag
      121 gacaccgccg aacacacaca agccctgtgc cccccaattt tgttgggggg cacaggcatt
      181 tgtgccgcat cagtcgaaaa gcgttaagcc ggcaggcttt ctgctcttct ccaactccag
      241 aagtacgcgt ttgcgatcca gcccgccccc atagccggtg aggctgccgc tggcgccgat
      301 cacccggtgg cacggcacga tgatcccaat cggattgcgc ccgttggcga gtcccaccgc
      361 gcgggacgca ccgggtgcgc cgatctgtgc ggcgatctct ccgtacgatc gcgtctcgcc
      421 gtacggaatg gtttgaaggg ccgcccagac gcgtcgctga aactctgtgc ctgtcaaggc
      481 gaggtcgacg tcgaaactgc tgagttcgcc ggcgaagtag gtgccgagct gctcgacggc
      541 gtcgggaaag gcgtcgtcat cttctaccca acctgcccgg tccggctcgt gcgtttgacc
      601 cgtcatccgc aggtgggcga gtgtgttgtc gcggccggcc aatgtcaggg ggccaactgg
      661 gctgtcgatg atgcgaaaat atatggcgct catggtttct ccttgagtgg ccagtggttg
      721 attgggtggt cgagggttgc ccacaggtgc tgtgtggcat acgagcgcca cgggcgccat
      781 cgcgtgctgt ggtcgttcaa cgcacggatg ccggttggta ggcccacgtg ctcggcggcc
      841 gcgcgaattc cgttgtcggc gacaggaaac gcatcagggt cgcccagccc ccgcatcgcg
      901 atcatttcag tggtccacgg cccaacgccg gggatatcca gaagctgacg ccgcgcgcgt
      961 tgccagtcgc atccgacacc gagggtaacc gcacccccgg ccagggcgtg cgccagtgca
     1021 atcacactgc gctgccgcga ctttgggaag gcaagatcgg caggatcgag gctggcgagt
     1081 cggtccaccg acggaaagac gtgggtcagt cctccgtctg ggtcggcgac cagtgtgcca
     1141 tgcgcctgga caatccgtgc gcaatgggtc cgggctgcgt tcatcgacac ctgctgcccg
     1201 atcaccactc gtatcgcgag ctcctgttcg tcgacagtgc gggggatccg ttggccgggg
     1261 tgcttaacta ccagcggggc caggtctcgg tcggtgccca ggaagtcgac caccgactgg
     1321 gggtcggcgt cgaggtccag caaccgccgg cagcgagcaa tcgcagtgga aagatcgcgg
     1381 aaatcgtgca accgcaacac gcatcgaaca tgatcgggtg tgggcgtcaa gctgacgatg
     1441 gcgttgcccc atggcagccg cagcgttcgt cggtaggccc cgtcgcgaac ctcttcgcag
     1501 ccggcaattg cgctggcggc caagtgcccg aacagcccct cgaacgcgaa cggcgtccgc
     1561 gtcggcaacc gcagcgcaac cgttcccgcg ccctcaccgg cggcatctgg accggcgcgc
     1621 cgagcggcct tctggcgcaa ctccgttgga gtgctggcgc acacctcgcg gatcgtgtcg
     1681 ttgaactggc ggatgctgga gaaccccgcc gcgaacgcga cgtcgctgaa cggcaggtcg
     1741 gtggtttcga tcagcactcg cgcggtctgc gtccgctggg cgcgggccag cgcgagcgga
     1801 cccgcgccca cctcggcctg aagcagccgc tcgagctggc gtggggtgta gcccaccttg
     1861 gcagccagtc cggtgacgcc gtcgcggtcc actgtgccgt cggcgatcaa ccgcatggcc
     1921 cgcgccacca cgtcaccgcg gacattccac tccggcgacc ccggcgatgc gtcgggccgg
     1981 cagcgcttgc agggccggaa gcccgcccgc tgcgcggccg ccgcggtggg atagaaacgc
     2041 acgttgcgag cgaacggcga tcgcgccggg caactgggcc ggcagtagat cttcgtggtc
     2101 agaaccgcgg tgacgaacca cccgtcgaac cgggtgtcct tggactggat cgcccgatag
     2161 cagccgccga agtcgtcgtg catacctcga caattacacg cgcccaccga cagcactggc
     2221 ggaaatccga catcacggtc tgcgcacgtg ttcctgatcg actgggatcg cgagccgggt
     2281 tggcttctcg cgcccgcgca gaacgacctg gtcgtccagt cgccaatgcc gccgttcgct
     2341 ttcgctggcc gcttcgacgg cggcgtcgga ggccagcaga cgctgcggat acgatttgga
     2401 aagttcgcag agccgcgccg cctcgttcac cgggtcgccg atcaccgtgt actcgaagcg
     2461 ttcgtaggcg ccgacattgc cggcgacggc cttgcccgca gcgaccccga ggccggccgg
     2521 acactccggc acttcctggc acagccggcg ggcaacggta cgtgcggccg ccagcgcgtc
     2581 atcttcaggt cgcgcgagcc gaaccggcgc gccgaacacc gccagcgagc cgtcgccttc
     2641 gaacttgttg agcagcccgc ggtgacggtc gacctcttcc acgatgaccg tgaagaaccg
     2701 gttgagctgt cctaccacct cgctgggggg cttgctgccc accagctgtg tggatccgat
     2761 catgtccacg aaaaacaccg cgacatggcg ttcttcgccg ccgagttcga gctcttgttc
     2821 ttcggccgcc gcggcaacct cacggcccac gtgtcggccg aacaggtcgc gaatgcgttc
     2881 gcgttcacgc aatccatgga ccatcgagtt gaagccacgc tgcagctcgc ccagttcggt
     2941 gccgtcgaac accacaaggt tgctgtcgag gtcgccatcc tcgacccgct ccagcgctgc
     3001 ccgcacgacc cggaccggtg tggcggtgat ccacgaaacg atcagcatca gcacgaagcc
     3061 gaagaccagc gcgatgatgc ccagaatcaa cacggcgatc ccgaactggg tctgggacat
     3121 gttccgtagc gacaacgcga acatcgtggc cagcacgatg cccagcaccg gcaccgccga
     3181 accgagcagc cacaccgtca tgatccggcc catgatgccc gcggccagtc gccgtggcgg
     3241 gtgcccggcc tccagcgcct gcgcggccac tgggcgtagc gcgaactcgg tgagcaggta
     3301 gcagccggtg gagacgacga tcccgctgaa accgacggcg aacagataac gaggaatgaa
     3361 cgccgtgtcg acgacgccgt acagcgtcgt tagcaatgcg gtgccgacgc cccacagcac
     3421 gaggtgcatc tcggccaagt gccatggggc gaggaaggtg ttccgctggt cctcccgagt
     3481 gggaggccgc tcctcgatgg cccagcgcag cgcgccgacc gtgcgtcgcg tcacccagaa
     3541 gatgcccact agcagcgcca ccagggtata tgccgggctg acgacccacg tcagccacca
     3601 cggagcttcg gtgaagatgc tcggcaccgg gaacgcgatc gtcaccagca gcaccgcgac
     3661 gccgatcccg atgaagttcg cggtcacgac gaacaccgtg aggatgacct ggatgcgcac
     3721 ccgtcgccgc gtttgactct cttcagcccg acccagcagc caggacccga agtcgggcgt
     3781 atccgggagc cggccgcttt gccgcgtcac ccgctcgagc acccggccca gccgctgagc
     3841 aaccgacaac ctggcaacca tggtggcgca agcctagttg ccagagtcgc ccctaaagtg
     3901 gaccagtgcg tctcgtgatc gcccagtgca ctgtcgacta tctcggacga ctcaccgcgc
     3961 atctgccatc ggcacggcgg ctgctgttga tcaaggccga tggatcggtg agcgtgcacg
     4021 ccgacgaccg agcgtacaaa ccgctgaact ggatgagccc gccgtgctgg ctcacggaaa
     4081 ccaccgagga ctcgtcggcg gtgtgggtgg tggagaacaa gaccggtgag cagttacgca
     4141 tcacgatcga agagatcgag cacgactcca ctcatgagct cggagtcgac cccggcttga
     4201 tgaaggatgg cgtcgaggcg catctgcagg agctgctggc cgagcacgtc gaattgctag
     4261 gaagcggata cacattggtg cgtcgcgagt acatgacgcc gatcggcccg gtcgacctgt
     4321 tgtgccgcga cgagcagggc cggtcggtcg ccgtcgaaat caagcggcgc ggggaaatcg
     4381 acggtgtcga acagctgacc cgatacctcg acctgctgaa ccgcgacagc ctgctggctc
     4441 cggtcagcgg ggtgttcgcc gcccagcaga tcaagcccca agcgcgcacg ctcgcggttg
     4501 atcgcggaat ccgttgcatc acattggatt atgatcaaat gcgcggtctg gagagcgacg
     4561 agtaccggtt gttctgacgt aggtcgttaa actggtgcga tgagacgacg gagccagcgg
     4621 atgcctcgac acgctccgtt gccgaccgct cgtaggatcg agaccggccc ggacggatac
     4681 gactacgagg tgcgtcccgt cgccgcagcg cttgccacca agtcctaccg ctgccccggc
     4741 tgtgaccacg aaatccattc cggcacagcc catgtggtgg tgtggcccgc gggcgcgggc
     4801 ggcgtggacg atcgtcggca ctggcacacc gcatgctgga ggcaccgggc cacgcggagc
     4861 ccgacgcgaa agtggtctta gcgaatcagg attcgggcgc cggctcgacc agctcgatca
     4921 gcacgccgcc ggcatccttg ggatggatga agttgatccg tgagttggcg gtgccgcgcc
     4981 ggggttcgtc gtagagcagc cggatgcctt ggtcttccag ccgcttggtg agttggtcca
     5041 gtttgctgac gcggtatgcg agctgctgga gcccgggccc gcgcttgtcc agaaacttcg
     5101 cgatcgccga cgagtcgctg atcggcgcca tcagctggac ttgggtgctg ccgaccggcg
     5161 cgccgcgcac cgagagcatt gcctcgcgga taccctgatc ctcgttgacc tcctcgtgca
     5221 gcacgatcat gccgaggtag tcgtgatacc acgcgatcgc agcgtcgagg tctgccactg
     5281 cgaccccaat gtggtcgatc cctgtcacca acgcactggc gaggacaggg cgggcatcaa
     5341 cctgctcggc ggtcatgacg tcaaggtaac ctaaacgaca aagattctgc agctccggtt
     5401 tgtggctgta ggcgccgaac cgacccctgg aggtagagat gacgacgtcg gtgatcgttg
     5461 ctggagcacg cacgccgatt ggcaagctga tgggttcgct gaaggatttc tccggctctg
     5521 atctgggcgg gatcgcgatc gccggggcgt tagagaaggc caacgtgcct gcttcgctga
     5581 tcgactacgt gatcatgggc caggtgctca cggccggcgc cggccagatg cccgcgcggc
     5641 aagccgcggt ggccggcggt atcggatggg acgtgcccgc gttgaccatc aacaagatgt
     5701 gcctgtcggg gatcgacgcc atcgcgctgg ccgatcagtt gatccgcgcc ggggaattcg
     5761 acgtcgtggt ggccggaggt caggagtcga tgacggccgc gccccacctg ctgataggca
     5821 gccgggcagg ctacaagtac ggcgacgtca cggtcctcga ccacatggcc tacgacggcc
     5881 tgcacgacgt gttcaccgac cagccgatgg gcgcgctcac cgagcagcgc aacgacgttg
     5941 accagttcac ccgcgccgag caggacagct acgccgccgg atcccaccag aaagccgccg
     6001 ccgcctggaa ggacggcgtg ttcgccgacg aggtggtgcc cgtcaagatt ccgcagcgta
     6061 agggcggcga tccgatcgag ttcggcgagg acgagagcat ccgcgcgaac accacagcgc
     6121 agtcgctatc cggcctcaag ccggcgttcc gcaaagacgg caccatcacg gccggttcgg
     6181 cgtcgcagat ttccgacggg gccgccgccg tcgttgtgat gaacaagcag aaggccgagg
     6241 aactcgggct gagctggttg gtggagatcg gcgcacacgg cgtggttgcc ggacccgatt
     6301 cgacgctgca gtcgcagcca gccaacgcga tcaagaaagc ggtcgcacgc gaggggattt
     6361 cggtagacca gctcgacgtc atcgagatca acgaggcgtt tgccgccgtc gctctggcct
     6421 ccatccgaga gctgggcgtg gacgcggaaa aggtcaacgt caacgggggc gccatcgccg
     6481 tcggtcaccc gatcgggatg tcgggcgcgc gcatcacgct gcacgccgcc ttggagctcg
     6541 cccggcgcgg ctccggctac ggtgttgcgg cgttgtgcgg cgcgggcggg cagggagacg
     6601 cgctgatcct gcgggccggt taaccggagg ttgctgggac gaccggtcgc ggtcgtggaa
     6661 atcgccgatg tgagattcgt cataccacgg gccgcacggg gtgcgaccgg ctgcgacgac
     6721 gccgcggacg acgtgacaaa cttgaccacg tgactcgtcc gcgtcccccg gtcgcgcccg
     6781 ccatggcggg tgccgttgac ctttccgccc tcaagcagcg cgccactcag agcagcgacg
     6841 ccggccgggc ggccggcgcg caggccggcg tcgagatcac cgagacgaac ttcgaagccg
     6901 aggtgctggc ccggtccaac gaggtgccgg tcgtggtggt gttgtggtcg ccgcgcagcg
     6961 atgcgtcgat tcagttggcc gatgcgttgg cgggtctggc ggcgaccgac gccggcacat
     7021 ggtcg
//
LOCUS       DASWBF010000035         5602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1120073,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000035 DASWBF010000000
VERSION     DASWBF010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120073"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VF874_01000"
     CDS             <1..249
                     /locus_tag="VF874_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9174714.1"
                     /translation="KSRLRRLDGDPLLSTWSEIVADMTATELPIDAGDAILAGRLPWE
                     HNDPFDRVIVAQAARRNLTIATHDVRILSAALTATLKV"
     gene            complement(368..2191)
                     /locus_tag="VF874_01005"
     CDS             complement(368..2191)
                     /locus_tag="VF874_01005"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX9174715.1"
                     /translation="MTAATIPGLDNAPTKHEGLLAWVREVAELTEPDRVVFTDGSEEE
                     CARLTEQLIAAGTFIKLNEHKKHNSYAANSDPSDVARVESRTFICCEREIDAGPTNNW
                     MDPAEMRGIMTDLYRGCMRGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMRTMT
                     RMGKAALDKMGDDGFFVKCLHSVGAPLEPGQKDVPWPCNDTKYITHFPETREIWSYGS
                     GYGGNALLGKKCYSLRIASAMAHDEGWLAEHMLILKLISPENKAYYIAAAFPSACGKT
                     NLAMIQPTVPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGVAPGTNWESNPNAMA
                     TMAAGNTVYTNVALTDDNDVWWEGLEREAHHLIDWKGNDWYERETETVAAHPNSRYCT
                     PMSQCPILAPEWDDPQGVPISAILFGGRRKTTVPLVTQANDWQHGVFIGATLGSEQTA
                     AAEGKVGNVRRDPMAMLPFLGYNVGDYFQHWIDLGKNSDESKLPKIFFVNWFRRGDDG
                     RFLWPGFGENSRVLKWIIGRVEGKASGQSTPIGTVPSAEDLDLDGLDGDPADVAEALA
                     VNPDEWREELPQIEEWFEFVGEKLPTGIKDEFDALKHRLAE"
     gene            complement(2322..3761)
                     /locus_tag="VF874_01010"
     CDS             complement(2322..3761)
                     /locus_tag="VF874_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174716.1"
                     /translation="MTERAADHQAAVDALVAAHHPGVGLGPSAQTRDVILVAGPWLAG
                     STSLAAALRDRLPDRVFVDAGELGDTDAPAAAIFVTSSVAPLTASDCALLDSAAASTD
                     LVIGAVSKIDVHRDWRDVLAADRKVVAAHDPRYADMAWVGVAAAPQLGEPRLDELATA
                     LEQGLAQSQLARRNRLRVWQNRLRDAARRHEDASLGHEARMTALKQERSQTLRRHRLA
                     KSERTISLRSRIQQAKVQLAYFARTRCASVRGELAEDAATLSPRRVRTFEKYVVKRVD
                     DVIEEVHDGVSEQLTDLATAFRLTPPADEPPPERPPIPRPPLTAANLETRLMMLLGAV
                     LGVGVALTLSRLFADVAPAYTAVGVAVGAAVGLAVAVWVVGMRRTLRDRAVLDRWVGD
                     VVGALRPVVEQHVATRMLTAESALTTEQAQRDDVDAATVADRVTAIDDELREHAVAVA
                     AASARRDRELPSFQRALEAVDQELNRSCE"
     gene            complement(3758..4675)
                     /locus_tag="VF874_01015"
     CDS             complement(3758..4675)
                     /locus_tag="VF874_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174717.1"
                     /translation="MGGQGHTIFTDRLARFAAEVDDPALSAITARIGADVTVAVHGRT
                     GVGRGAVARALARAGLAVTRDGDVDVHVVAEVVKPEDQVAITASAAERPTLVVLNKAD
                     LVTGARALCARYRALTGVTTVPMVAHLAHVQVDDELLSALRTASPAPSRRLLDTFDTY
                     GIAQALLAVRAGADAAALQRVLRVRSGVDGVVAALAPMLAEAGYQRLRWARTALEAIA
                     ATGDERLAALLHDDDTAIACMAAAVDVVQAAGMAVDPGDDPAAHVRRAAHWHLYRSGP
                     VNAVHTACGADIVRGSLRLWRRAQSGAVS"
     gene            4779..5522
                     /gene="trmB"
                     /locus_tag="VF874_01020"
     CDS             4779..5522
                     /gene="trmB"
                     /locus_tag="VF874_01020"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX9174718.1"
                     /translation="MQTQRDGLSDLESPPHLHRRVTSFRSRRASLTDAQQRTWARLWP
                     ELGTNARGELLDTGRWFGRSAPVVLEIGCGTGTSTAAMAEEEPHVDVVAVEVYRKGLA
                     QLLGAVDREQITNIRLIRGDGLDVLEHMFGPESLTGVRVFFPDPWPKSRHHKRRLLQP
                     GTVALIADRLERGGILHVATDHEGYAEQIAEVGDAEPRLRRVPPGASTPISVRRPTTK
                     YESKAQDAGNAVAELLWEKAPPDARRQMA"
BASE COUNT          857 a         1977 c         1868 g          900 t
ORIGIN      
        1 aaatcccggc tgagacgcct cgacggcgac ccgctgctgt cgacctggtc ggagatcgtc
       61 gcggacatga ccgccaccga actaccgatt gacgccggtg acgccatcct ggccggccgg
      121 ctgccgtggg aacacaatga ccccttcgac cgcgtgatag tggcccaggc cgcgcggcgc
      181 aacctcacca tcgccacaca tgacgtccgg atactttccg ccgctctcac cgcgaccctg
      241 aaggtgtaag cgggccgagg tgaatacctg gtctaccgtc ggtcatgctg agtgcgccct
      301 gacggctcgg cgttctgagc ggcgcggctg tgcgaggcca agcccgcgcg gacctatcgc
      361 cgcgagccta ttcggcgagg cggtgcttga gtgcgtcgaa ctcgtccttg atgccggtgg
      421 gcagcttctc tccgacgaac tcgaaccact cctcgatctg cggcagctct tcgcgccact
      481 cgtcggggtt gacggccagc gcctcggcca cgtcggcggg gtcgccgtca agtccgtcga
      541 gatcgaggtc ctcggcggat ggcacggtcc cgatcggcgt ggactggccg gacgccttgc
      601 cctcgacgcg cccgatgatc cacttcagca cccggctgtt ctcgccgaag cccggccaca
      661 ggaagcggcc gtcgtcgccg cggcggaacc agttcacgaa gaagatcttc ggcagcttgg
      721 actcgtcgga gttcttgccg aggtcgatcc agtgctggaa gtagtcgccg acgttgtagc
      781 cgaggaacgg cagcatcgcc atcgggtcgc ggcgcacgtt gccgaccttg ccctcggccg
      841 cggcggtctg ctcactaccc agcgtcgcgc cgatgaatac gccgtgctgc cagtcattgg
      901 cctgcgtgac cagcggcacc gtggtcttgc gccggccgcc gaacaggatc gccgagatcg
      961 gtacgccctg cgggtcgtcc cactccggtg ccaggatcgg gcactgcgac atcggcgtgc
     1021 agtagcggga attcgggtgc gcggcaaccg tttcggtctc gcgctcgtac cagtcgttgc
     1081 ccttccagtc gatgaggtgg tgggcctcgc gctcaaggcc ctcccaccac acgtcgttgt
     1141 cgtcggtcag cgcgacgttg gtgtagacgg tattgcctgc cgccatggtg gccatggcgt
     1201 tggggttgga ctcccagttg gtgcccggcg cgacgccgaa gaacccgaac tccgggttga
     1261 ccgcgtacag tcgcccatcc ttgccgaacc gcatccaggc gatgtcgtca ccgagcgttt
     1321 cggcgcgcca accgggaacg gtcggctgga tcattgcgag gttggtcttg ccgcaggccg
     1381 acgggaatgc ggccgcgatg tagtaggcct tgttctccgg cgagatcaat ttgaggatca
     1441 gcatgtgctc ggcgagccag ccctcgtcgt gcgccatcgc cgacgcgatc cgcagggagt
     1501 agcacttctt gcccagcagc gcgttgccgc cgtagcccga gccgtagctc cagatctcgc
     1561 gggtctccgg gaagtgggtg atgtacttgg tgtcgttgca cggccacggc acgtctttct
     1621 ggccgggctc gagcggggcg ccgaccgagt gcaggcactt gacgaagaac ccgtcgtcac
     1681 ccatcttgtc cagggcggcc ttgcccatcc gggtcatcgt gcgcatcgac accacgacgt
     1741 actcggagtc ggtgatctcc acgcccagct tcgggtcttc ggcgcccagc gggcccatgc
     1801 agaacgggac cacgtacatc gtgcggccgc gcatgcaccc ccggtacagg tcggtcatga
     1861 tgccccgcat ctccgcaggg tccatccagt tgttggtcgg tccggcgtcg atctcccgct
     1921 cgcagcatat gaacgttctc gactcgacgc gcgcgacgtc cgacgggtcc gagttggcgg
     1981 cgtaggagtt gtgcttcttg tgctcgttga gcttgatgaa ggtgccggca gcaatgagct
     2041 gctcggtcag ccgggcgcac tcctcctctg agccgtcagt gaacaccacc cggtccggct
     2101 cggtgagctc ggcgacttcc cgcacccacg cgagcaaccc ctcgtgtttc gtcggtgcgt
     2161 tgtccagacc cggaatggtc gctgcggtca tcagtcactc ctgctgtgtg gttctatgcc
     2221 aggacgtaag ccacgccgca cctacacggc agcggcgggt tgcccctaaa gtagaggtta
     2281 acgccatggt cacaactctg gttaaccgga gtgacgccga attactcaca ggaacgattc
     2341 agttcctgat ccaccgcctc cagcgcccgc tgaaacgacg gcaactcacg atcacgcctc
     2401 gccgaggccg cggccacggc gaccgcgtgt tcccgcagtt cgtcgtcgat cgcggtgaca
     2461 cggtcagcga ccgtcgccgc gtccacgtcg tcgcgttgag cttgctcggt ggtcagggcc
     2521 gactcggcgg tcagcatcct ggtggccacg tgctgctcga cgacagggcg cagcgcaccg
     2581 accacatcgc cgacccagcg gtccagcaca gcgcggtcac gcagcgtgcg gcgcatgccg
     2641 accacccaca ccgccaccgc cagcccgacc gctgcgccta cggccacccc gacggccgtg
     2701 tacgccggag ccacgtcggc gaacagtcgg ctcagcgtga gcgccacccc caccccgagc
     2761 acggcgccga gcagcatcat cagccgggtt tccaggttgg ccgctgtcag gggcggccgc
     2821 ggtatcggcg gccgttccgg cgggggttcg tcggctggcg gagtcagcct gaacgcggtc
     2881 gccaggtcgg tgagctgctc gctgacgccg tcatggacct cctcgatgac gtcgtccact
     2941 cgcttgacca cgtacttctc gaaggtccgc acccggcggg ggctcaacgt cgcggcgtct
     3001 tcggccagct cgccgcgcac cgacgcgcac cgggtgcgcg cgaagtaggc cagctgcacc
     3061 tttgcctgct gaatgcggct gcgcagactg atggtccgct ccgacttcgc cagtcggtgc
     3121 cgacgcaggg tctggctgcg ttcctgtttg agcgccgtca tccgggcttc gtgccccaga
     3181 ctcgcgtcct cgtggcgccg cgccgcgtca cggaggcggt tctgccacac ccgcagtcgg
     3241 ttgcgtcgcg ccaactggct ctgcgccaag ccctgttcga gcgcggtggc cagctcgtcc
     3301 agccgtggtt ccccgagttg cggcgccgcg gccacgccga cccacgccat gtccgcgtac
     3361 ctggggtcgt gcgcggcgac gaccttgcgg tcggcggcca gaacgtcccg ccagtcgcgg
     3421 tgcacgtcga ttttgctcac cgcgccgatc accaagtcgg tgctggccgc tgcggagtcg
     3481 agcagcgcgc agtcggaggc ggtgagcggc gcgaccgatg aggtgacgaa gatcgccgcc
     3541 gcgggggcgt cagtgtcacc caactccccc gcgtcgacga aaacccggtc cggcaggcgg
     3601 tcgcgcagcg cggcggcgag gctcgtcgag cccgcaagcc acgggccggc gaccaggatg
     3661 acatcgcgtg tctgcgcgct ggggcccagg cccacaccgg ggtgatgcgc cgcgaccagt
     3721 gcgtcgacgg cggcctggtg gtcggccgcg cgttcggtca tgacaccgca ccgctctgcg
     3781 cacggcgcca tagccgcagc gacccgcgga cgatgtcggc gccgcacgcg gtgtgaacgg
     3841 cgttgacggg cccgctgcga tacaggtgcc agtgcgcggc ccggcgcaca tgtgcggccg
     3901 gatcgtcacc tgggtctacg gccatgccgg cagcttgcac tacgtcgacc gccgctgcca
     3961 tacatgcgat cgccgtgtcg tcgtcatgca ggagcgccgc gaggcgctcg tcgcccgtcg
     4021 ctgcgatcgc ctctaaagcc gtacgcgccc accggagccg ctgatacccc gcctcggcga
     4081 gcatcggcgc cagcgccgcc acgaccccgt cgacaccgct gcggacccgc agcacccgct
     4141 gcaacgcggc ggcgtccgca ccggcacgca cagccagcag cgcctgcgcg atgccgtagg
     4201 tgtcaaatgt gtcgagcaac cgccggctgg gcgcgggcga cgcagtccgc aacgcgctca
     4261 gcaactcgtc gtcgacctgc acgtgcgcga ggtgggcgac catcggcacg gtcgtcactc
     4321 cggtcagcgc gcggtagcga gcacaaagtg cgcgcgcgcc ggtgaccaga tcggccttgt
     4381 tgagcacgac cagggtgggc cgctccgccg ccgacgccgt gatcgcgacc tggtcctcgg
     4441 gcttgaccac ctcggcgacg acgtgcacgt cgacgtcacc gtcgcgcgtt acggctagcc
     4501 cggcgcgtgc taatgcacgg gcgacggcgc cgcggccgac gccggtcctg ccgtgcactg
     4561 ccacggtcac gtcggcgcca atccgcgcag tgatcgcgct gagcgctgga tcgtcgacct
     4621 cagcagcgaa ccgggccagt cggtcggtga aaatcgtgtg gccttgtccg cccattgatc
     4681 cccccagctg ccggcgctcc taccagtaaa aggcaactgt tgggtatcaa tctgcaccac
     4741 atattcaggc cgcctacttc atgagccacc attgacggat gcagacgcag cgcgacgggc
     4801 tttccgacct ggagtcaccg ccgcatctgc accgccgcgt gacgagtttc cgctcgcggc
     4861 gcgcaagcct caccgacgcg caacagcgga cctgggcgcg gctgtggccc gagttgggca
     4921 caaacgcccg cggtgagctg ctcgacaccg gccggtggtt cggccgctca gcgcccgttg
     4981 tcctcgaaat cggttgtggc acagggactt ccactgctgc gatggccgag gaggagccgc
     5041 acgtcgacgt ggtggcggtc gaggtgtacc gcaaagggct ggcgcagctg ctcggggccg
     5101 tcgaccgtga gcagatcacc aacatccggc tgatccgcgg cgacggcctc gacgtgctgg
     5161 agcacatgtt cggccccgag tcgctgaccg gtgtgcgggt gttcttcccc gacccgtggc
     5221 cgaagtcgcg ccaccacaag cgtcggctgc tgcaaccggg caccgtcgcg ctgatcgccg
     5281 accggctcga gcgcggtgga atcctgcacg tcgccaccga ccacgagggt tatgccgagc
     5341 agatcgcgga ggtcggagac gccgaaccgc ggttgcgccg cgtaccaccc ggggcatcaa
     5401 cgccgatctc ggtccgacgg ccgaccacga agtacgagtc caaggcgcag gacgccggaa
     5461 acgccgtcgc cgagctgctc tgggagaagg cgcccccgga cgcgaggagg caaatggcat
     5521 gagcgtgacc gaacaaatag aagagatccc cgccagcccg gccgcggaca tgcaggacac
     5581 ccagccggcg ccgcggcggg tg
//
LOCUS       DASWBF010000036         7250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_330950,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000036 DASWBF010000000
VERSION     DASWBF010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330950"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VF874_01025"
     CDS             complement(<1..556)
                     /locus_tag="VF874_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9174719.1"
                     /translation="MSYTVNGQTFDEEPAPGQCLRTFVRSLGWFGVKKGCDAGDCGAC
                     TVWLDGNPVHSCITPAFRADGREVTTVEGLGSPDQMHPLQRQFRDAPGFQCGFCTPGM
                     IMTAASFSDEQKMNLPRSLKGNLCRCTGYRAIEDAIRGVAGIEDAEAGKAVGVSVGAP
                     AGTEVVTGTAEYTMDTHIDGMLHLK"
     gene            complement(553..1380)
                     /locus_tag="VF874_01030"
     CDS             complement(553..1380)
                     /locus_tag="VF874_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016881051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9174720.1"
                     /translation="MDLNTVTSVIRRPSERPGEQWRQGDVWLAGGTWLFSEPQPDVTR
                     LIDLTELGWPSLVPSREGLEIGATCTIRELYAFAPPLDWRAASLFSTCCEAFLASFKV
                     WNSATVGGNICMSLPAGPMITLAVALDATYTLWEANGSERTVAASDFVTANHENVLAR
                     GEILRSIHIPIDALRKHHAHRRFTLTHLGRSTVFLIGTRAVDERGLTLTITAGTTRPV
                     QVRFDDAPDAGMVRDAIDAIPSDIWFDDANGTPQHRRHLTEHFAEEIRAELTEGGKR"
     gene            1607..1900
                     /locus_tag="VF874_01035"
     CDS             1607..1900
                     /locus_tag="VF874_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174721.1"
                     /translation="MKSSRSARRLAAFAAIFVIAGMGALTAACGGNEKEEPTTTTTTA
                     TTTAPPTTPPPPSPTENMPPPTGGNVFTPNPIPNPPQTTHRQREYPYPGAGPT"
     gene            complement(1923..2567)
                     /locus_tag="VF874_01040"
     CDS             complement(1923..2567)
                     /locus_tag="VF874_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX9174722.1"
                     /translation="MESTVLPMFPLEVTLLPDDDLPLRIFEPRYSQLVADCLAADDPV
                     FGVVLIAAGREVGGGDNRYDVGVLARIVEHRALGDGKYTLRCRVSERLRVLRWLPDDP
                     YPRAEVQSWTDEPGAAVSDDAIQQLENRVMSLFERIAAARGAELPPRDMLFAGVALAE
                     GPGEKLYALASTVPMGQADRYAVLAAPSPDARLRALSEAVETMAAMIDFQLRGE"
     gene            complement(2581..3699)
                     /locus_tag="VF874_01045"
     CDS             complement(2581..3699)
                     /locus_tag="VF874_01045"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX9174723.1"
                     /translation="MAKRRIDFAASPRPTVGVEWEFALVDAETRDLSNEAATVIAEIG
                     ENPRVHKELLRNTVEFVTGICDTTPQAMEDLRSTLLAARDIVRGHGMELFCAGTHPFA
                     KWSAQKLTDAPRYAELIKRTQWWGRQMLIWGVHVHVGISSAHKVMAIISSLLNQYPHL
                     LALSASSPFWSGEDTGYASNRAMMFQQLPTAGLPFQFQKWSQFEGFVHDQKKTGIIDH
                     VDEIRWDIRPSPHLGTIEVRIFDGVSNLRELAALVALTHCLVVDLDRRLDAGESLPVM
                     PPWHVQENKWRAARYGLDAVIILDADSNERLVTEDLEDLLTRLDPVARSLGCADELAS
                     VAQIAAVGASYQRQRRVAEDNDGDLLAVVDALVGELDI"
     gene            complement(3707..4375)
                     /locus_tag="VF874_01050"
     CDS             complement(3707..4375)
                     /locus_tag="VF874_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX9174724.1"
                     /translation="MVKPTTFVAALLAVPALALSACSTNQNPSTEPGTTPPVWTGSPS
                     PSGPTTEAPDGQGKKLTAQLSTPDGKPVATATFDFAGGFATITVETIGDGILSPGFHG
                     MHIHSVGKCEANSVAPTGGPAGDFLSAAGHFQAPGHTTHPASGDLSSLQVRADGAAKL
                     VTTTDTFSAEQLLAGQGTAIIIHEKPDNFANIPDRYSDTGADAETLATGDAGKRVACG
                     VITG"
     gene            complement(4386..4835)
                     /locus_tag="VF874_01055"
     CDS             complement(4386..4835)
                     /locus_tag="VF874_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX9174725.1"
                     /translation="MNQRLPQSGGLPLRAMVMVLLFLAVLFLLLGINAVLSDGDSDNQ
                     ATSVTTTTTSPAPAKTAVHVYNISDVEGAAARTGDQLTQAGWNATVEPEGLELPGVTV
                     TTVYFTDAPGERESADEVATLLGNAPVEPRRPELVDLPPGVIVAVTG"
     gene            complement(4876..5184)
                     /locus_tag="VF874_01060"
     CDS             complement(4876..5184)
                     /locus_tag="VF874_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEX9174726.1"
                     /translation="MDGAMARAKRSGDDAEPVDGLTRREHDILAFERQWWKFAGSKEE
                     AIRELFAMSATRYYQVLNALVDRPEALAADPMLVKRLRRLRASRQKARAARRLGFELT
                     "
     gene            5298..5891
                     /locus_tag="VF874_01065"
     CDS             5298..5891
                     /locus_tag="VF874_01065"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9174727.1"
                     /translation="MAVLPIRIVGDPVLHKPTSPVEVGPDGSVPEDVRKLIADMYETM
                     AVANGVGLAANQVGVSLRLFVYDCAEARGMTVRRKGVVINPVLETSEVPETMPDPEND
                     DEGCLSVPGESYPTGRAKWARVTGFDAEGAPVALEGEDLFARMLQHETGHLEGMLYLD
                     RLLGRHARSAKRMLKSHGWGVPGLSWTPGEVPDPFGH"
     gene            5893..6663
                     /locus_tag="VF874_01070"
     CDS             5893..6663
                     /locus_tag="VF874_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9174728.1"
                     /translation="MRIATWNVNSIRARVDRVTNWLERAGVDVLAMQETKCAEDQFPT
                     MPFAAIGYEVVHCGFNQWNGVAIASRVGIDDVQVGFEGQPSAEARALGATCGGVRVWS
                     LYIPNGRVPDSPHYYYKLEWLAALRDTARNWLVDDPSAQIALVGDWNIAPTDDDVWSV
                     EFYKHSTHVTEPERRAFREIVDAQFADVVRPFAPGPGVYTYWDYTQLRFPKRQGMRID
                     FILGSPALAERVTHAEIVREERKGKAPSDHAPVLVELG"
     gene            complement(6671..6970)
                     /locus_tag="VF874_01075"
     CDS             complement(6671..6970)
                     /locus_tag="VF874_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174729.1"
                     /translation="MIFTAPSGRAYTTKPGGSLFFPILATPTGEVVIDKLTAEPGEGR
                     GLMMPRRRRTRKQERDERIAAQRAINEQLLRRQRWLFAELEKRRAARAANDPPPF"
BASE COUNT         1201 a         2403 c         2418 g         1228 t
ORIGIN      
        1 ccttcagatg caacatgccg tcgatgtgcg tgtccatcgt gtattcggct gtgccggtga
       61 cgacttcggt gcccgcgggg gcgccgacgc tgacgcccac cgccttgccg gcctcggcgt
      121 cctcgatccc ggcgaccccg cggatggcat cttcgatcgc gcgatagccg gtgcagcggc
      181 acaggtttcc cttcagcgag cgcggcaggt tcatcttctg ctcgtccgag aacgacgccg
      241 cggtcatgat catgcccggc gtgcagaacc cacactggaa gccgggcgca tcacgaaact
      301 gccgctgcag cggatgcatc tggtccgggg aacccaggcc ctcgaccgtg gtcacctcgc
      361 gaccgtcggc gcggaacgca ggcgtgatgc agctgtgcac cggattgccg tcgagccaca
      421 ccgtgcacgc gccgcaatcg ccggcgtcac agcccttctt gacgccgaac caaccgagcg
      481 agcggacgaa cgtgcgcagg cactgacccg gcgccggctc ctcgtcgaag gtctggccgt
      541 tgacggtgta gctcatcgct taccgccttc cgtcagctcg gcccggattt cttcggcgaa
      601 gtgctccgtc aggtgccggc ggtgttgcgg ggtgccgttg gcgtcgtcga accagatgtc
      661 cgacgggatc gcgtcgatcg cgtcacgcac catgccggcg tcgggcgcgt cgtcgaatct
      721 cacctgcacg ggacgggtgg tgcccgccgt gatggtcaac gtcaggccgc gctcgtcgac
      781 ggctcgggtg ccgatcaaga acactgtcga gcggcccaag tgggtcaggg tgaagcgccg
      841 atgcgcgtgg tgcttgcgca gcgcgtcgat cggaatgtgg atgctgcgca ggatttcccc
      901 gcgagccagc acgttctcgt ggttggctgt cacgaaatcg cttgccgcaa cggtacgttc
      961 agacccattg gcttcccaca gcgtgtacgt cgcgtccagc gccaccgcca gcgtgatcat
     1021 cgggccggct ggcagcgaca tgcagatatt gccgccgacg gtggccgagt tccacacctt
     1081 gaacgaggcc aggaatgctt cacaacacgt gctgaacaac gacgcggcgc gccaatcaag
     1141 cggtggcgca aaggcataca gctcgcggat cgtgcacgtg gcaccgatct ccaggccttc
     1201 cctggacggc accagactgg gccagcccag ctcggtgaga tcgatcaggc gggtcacgtc
     1261 cggctgcggt tcggagaaca gccaggtgcc tccggcgagc cagacatcac cctggcgcca
     1321 ctgctctccc ggtcgttccg acggtcggcg aatgacgctc gtgacggtgt tgaggtccac
     1381 cggatcgccc ccgcatcgcg cgattttgca agacgtgtcg actgtaaacg gcacgatcag
     1441 cgttgggaca ttattcaccg atccgtcgac tacttccgcg ggccaggtgt taccaatcaa
     1501 tgacggccga cccgtaacga cccaagccca caccagggca gacgggacga gtgtagggtc
     1561 cagctagcag gtaccgggca accggcggat agataggaat ggtctcatga aatcgagtcg
     1621 gtcggctcgt cgtctcgccg ctttcgcggc gatcttcgtg atcgccggga tgggcgcact
     1681 caccgcggcc tgtgggggca acgagaagga agagcccacg accaccacca ccacagcgac
     1741 caccactgcg ccgccgacca ccccgccgcc gccttcaccg accgagaaca tgccgcctcc
     1801 caccggcggc aacgtgttca ccccgaatcc gatcccgaac ccgccgcaga ccacgcaccg
     1861 gcagcgcgag tacccgtacc cgggcgcggg cccgacctga gcacacctcg agctcgtggg
     1921 gcctattcgc cccgcagctg aaagtcgatc atcgcggcca tcgtctcgac ggcctccgac
     1981 agcgcccgca gccgcgcgtc cggggacggg gcagccagca ccgcgtagcg atcggcctga
     2041 cccatcggca ccgtcgacgc caacgcgtac aacttctcgc cgggaccctc cgccaacgcg
     2101 acaccggcga atagcatgtc ccgcggcggt aactcggcgc cgcgcgccgc agcgatgcgc
     2161 tcgaacaacg acatcacccg gttctccaac tgctggatcg cgtcgtcgct caccgccgcg
     2221 ccgggctcgt cggtccagct ctgcacctcg gcacgcggat acgggtcgtc aggtaaccag
     2281 cgcagcaccc gaaggcgttc tgaaacccgg caccgcagcg tgtatttccc atcgccgagg
     2341 gctcgatgct cgacgatgcg ggccagcaca ccgacgtcgt agcggttgtc cccgccgccg
     2401 acctcacgcc cggcggcgat cagcaccacc ccgaacaccg gatcgtcggc ggccaggcag
     2461 tcggcgacca gttgtgaata gcgcggttcg aagatccgca gcggcagatc gtcgtcggga
     2521 agcagcgtca cctcgagcgg aaacatcggc agcacagtgc tttccatcag atgctttcca
     2581 tcagatgtcc agctcaccga ctagcgcatc gacgaccgcg agcaggtcac cgtcgttgtc
     2641 ctcggcgact cgccgctggc gctgatacga cgcgccgacc gcggcgatct gcgcgaccga
     2701 cgcgagttcg tcggcgcaac ccagcgaccg tgccaccggg tccagccggg tgagcagatc
     2761 ctcgaggtcc tcggtgacca gccgctcgtt gctgtcggcg tccaggatga tcaccgcgtc
     2821 caggccgtaa cgtgctgcgc gccacttgtt ctcttgcacg tgccacggcg gcatcacggg
     2881 caacgactca ccggcgtcga gccgccggtc gaggtccacc accaggcagt gcgtcaacgc
     2941 cacaagcgcc gccaactcgc gcagattcga gaccccgtcg aagattcgca cctcgatcgt
     3001 acccaaatgc ggtgacggcc tgatgtccca acggatttcg tcgacgtggt cgatgattcc
     3061 ggtcttcttc tggtcgtgga caaacccctc gaactggctc cacttctgga actggaaggg
     3121 cagaccggcc gtcggcagct gctggaacat catcgcgcga ttgctcgcgt atccggtgtc
     3181 ctcgccgctc cagaacggcg acgacgcgct tagcgcgagc aggtgcgggt actggttcaa
     3241 cagcgacgag atgatcgcca tcaccttgtg cgccgacgaa atgcccacgt gcacatgcac
     3301 tccccagatc agcatctgcc gaccccacca ctgggtgcgt ttgatcagtt cggcgtaccg
     3361 gggagcgtcg gtcagcttct gcgccgacca tttcgcgaac gggtgtgtgc ccgcacagaa
     3421 cagctccatg ccgtgcccgc ggacgatgtc gcgcgcggcg agcaaggtcg agcgcaggtc
     3481 ttccatcgcc tgcggtgtgg tgtcgcagat gccggtgacg aattcgacgg tgttgcgcag
     3541 caactccttg tgcacccgcg ggttttcgcc gatttcggcg atcaccgtgg cggcttcgtt
     3601 ggagagatcc cgggtttcgg cgtcgacgag cgcgaactcc cactcgaccc cgaccgtggg
     3661 ccggggcgat gcggcgaagt cgatccggcg tttggccagc ggcttgctag ccggtgatga
     3721 caccgcaagc cacccgcttg ccggcgtcac cggttgcgag cgtctccgcg tccgcacccg
     3781 tgtcggaata gcggtccggg atattagcga agttgtccgg tttctcgtgg atgatgatcg
     3841 cggtgccctg accggcgagc agttgctccg cgctgaatgt gtccgtggtg gttaccagct
     3901 tcgcggcacc gtcggcgcgc acctgaagcg agctcaggtc cccgctcgcg ggatgggtgg
     3961 tgtgcccggg cgcctggaag tgcccagcgg cagaaaggaa gtcaccggca ggtccgccgg
     4021 tcggcgccac ggagttggcc tcgcacttgc ccacggagtg gatgtgcatg ccgtggaagc
     4081 cgggcgacag gatgccgtcg ccgatcgtct cgacggtgat cgtggcgaag ccgccggcga
     4141 agtcaaacgt ggctgtcgcc accggcttgc cgtcgggggt cgacagttgg gcggtcagct
     4201 tcttcccctg accgtcgggg gcctcggtgg tcggtcccga cggtgacggc gaaccggtcc
     4261 acaccggcgg cgtggtcccc ggttcggtgc tggggttttg gttggtgctg caggcgctca
     4321 gcgccagagc ggggacggcc aacagggcgg caacgaaggt ggtgggcttg accatggcag
     4381 tgagcctatc ccgtgaccgc cacgatcacg ccgggcggca ggtccacgag ttcgggccgc
     4441 cgcggctcca ccggcgcgtt cccgagcagc gtggccacct cgtccgccga ttctcgctcg
     4501 ccgggagcgt cggtgaagta cacagtcgtg accgtcacgc cgggcagctc gaggccctcc
     4561 ggctcgaccg tggcattcca gccggcttgc gtcagctggt caccggtgcg ggcggcggcg
     4621 ccctccacat cagagatgtt gtagacgtgc acggcagtct tcgccggtgc cggcgacgtc
     4681 gtggtggtgg ttaccgatgt cgcctgattg tcggagtcgc cgtcggagag caccgcgttg
     4741 attcccagca gcaggaacag caccgcgagg aacagcagca ccatcaccat ggcccgcagc
     4801 ggcagtccgc cggactgggg taagcgctga ttcatcgtgc ccactgtagc caggccacgg
     4861 ggtggccccg aaagctcagg tcagctcgaa cccgagccgg cgcgcggccc tggccttctg
     4921 ccggctggcc cgcagccggc gcagccgttt caccagcatc gggtccgcgg ccagcgcctc
     4981 ggggcggtcc accaacgcgt tgagcacctg gtagtaacgg gtggccgaca tggcgaacag
     5041 ttccctgatg gcttcttcct tggagccggc gaatttccac cactgccgct cgaatgccag
     5101 gatgtcgtgc tcacgacgcg tcagcccgtc gacaggttca gcgtcatccc ccgaccgctt
     5161 tgcccgcgcc atggcgccgt ccatatcgtc ccttctagcg aacgaatgac atccgtgtgt
     5221 cggcgaacat tcaatcacgg ttggccggtg ccgcacgtca gccgagcccg cgagtcggct
     5281 gactaagctg cacaaccgtg gcagtcctcc ccatccggat cgtcggagat cccgtcctgc
     5341 acaagccgac cagccccgtg gaggtcggcc cggacggttc ggtgccggag gacgtgcgca
     5401 agttgatcgc ggacatgtac gagacgatgg cggtcgcgaa cggagtcggc ttggcggcca
     5461 atcaggttgg cgtgtcgctg cgtctgttcg tctacgactg cgcggaagcc cgcggcatga
     5521 cggttcgtcg caaaggtgtg gtgatcaacc ccgtgctcga aacctccgag gtgcccgaga
     5581 cgatgccgga ccccgaaaac gacgacgagg gctgcctttc ggtgccgggc gagtcgtacc
     5641 cgacaggccg cgcaaagtgg gcccgcgtca ccggattcga cgccgagggc gcaccggtcg
     5701 cgctggaagg cgaggatctg ttcgcccgga tgctgcagca cgagaccggg catctcgagg
     5761 gaatgctgta tctcgaccgc cttctcggac ggcacgcgag aagcgcgaaa cgcatgctga
     5821 aatcccacgg ttggggcgtg ccggggctgt cgtggacacc gggcgaggtg cccgatccgt
     5881 tcgggcactg acatgcgcat cgccacctgg aacgtcaact cgatccgcgc ccgcgtcgat
     5941 cgcgtcacga actggttgga gcgcgccggc gtcgacgtgc tggcgatgca ggagaccaag
     6001 tgcgctgaag accagttccc gacgatgccg ttcgcggcca tcgggtatga ggtcgtgcat
     6061 tgcggcttca accagtggaa tggcgtggcg atcgcgtcgc gggtgggcat cgacgacgtg
     6121 caggtcggct tcgagggcca gccgtcggcg gaagcgcgcg cgctgggggc gacgtgtggc
     6181 ggtgtgcggg tgtggagctt gtacattccg aacggtcggg ttccggattc gccgcactac
     6241 tactacaagc tggaatggct tgcggcgctg cgtgatacgg cgcgtaactg gctggtcgac
     6301 gacccgagcg cccagatcgc actcgtcggg gactggaaca tcgcgcccac tgacgacgac
     6361 gtgtggagtg tggagtttta caagcacagc acgcacgtca ccgagcctga gcggcgggcg
     6421 ttccgcgaga tcgtcgacgc ccaattcgct gacgtggtgc ggccgttcgc ccctgggccg
     6481 ggggtgtaca cgtactggga ttacacgcag ctgcgcttcc ccaagcgcca gggcatgcgc
     6541 atcgacttca ttctcggctc acccgcgcta gcggaacgcg tgacgcacgc ggaaatcgtc
     6601 cgggaggaac gcaagggcaa ggcacccagc gatcacgccc cggtgctcgt cgaattgggg
     6661 tgattcccca tcagaacggt ggtgggtcgt tggccgcgcg ggctgctcgg cgtttttcga
     6721 gttcggcgaa cagccaccgt tggcgtctga ggagctgctc gttgatggcc cgttgggcgg
     6781 cgatacgttc gtcgcgctcc tgctttcggg tgcgcctgcg tcgcggcatc atcagcccgc
     6841 gtccctcccc tggctctgcg gtgagcttgt cgatcaccac ttcgcctgtc ggcgtggcca
     6901 agatcggaaa gaacaggctg ccaccgggtt tggtggtgta ggcgcgcccg ctaggagcgg
     6961 tgaagatcac cgtgccgtcg ggcagctgct tgtccgccca gcccgatcgg ccggtgtaga
     7021 aggttttgag caggtgatgg cagcgactcg ccgaagtact gtttagatga cgcgccgagt
     7081 gcgaagattg tcgcagctct gggggtaacc gatcagggtg aggcttactc gttgagagga
     7141 ggaggcgatg tacgcgacgg tacgccgcta tgaggggatc accgaccccg cagaggtcgg
     7201 gcggcgcgtc agggagggtt tcgttcctct gctcagggag atcgagggct
//
LOCUS       DASWBF010000037         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_398502,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000037 DASWBF010000000
VERSION     DASWBF010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398502"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VF874_01080"
     CDS             complement(<1..562)
                     /locus_tag="VF874_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174730.1"
                     /translation="MHKIVKRAGAGAVLGGSLLFAGGIGIASAAPGTNVDDQLVNLGV
                     GNADFLQGVNVDVASQIAGLLCGTGVTGTAANPGAATNGPNATGMSANETNTNVRDIT
                     ALANQVDAGQIPTTTCDSAQGVVTIAQNGPVTSPNANNVPTNTPNANTAPATGPNAEN
                     AVDQPNGAQPAEPTAPMTPTSGLTPAS"
     gene            complement(811..1254)
                     /gene="rpsI"
                     /locus_tag="VF874_01085"
     CDS             complement(811..1254)
                     /gene="rpsI"
                     /locus_tag="VF874_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9174731.1"
                     /translation="METTEVTTEAPAAREPVVIDRPIQTVGRRKEAVVRVRLVPGTGK
                     FDLDGRTLEDYFPNKVHQQLIKAPLVTVDRVDSVDIYAHLDGGGPSGQAGALRLAIAR
                     ALILVQPEDRPALKKAGFLTRDPRATERKKYGLKKARKAPQYSKR"
     gene            complement(1308..1751)
                     /gene="rplM"
                     /locus_tag="VF874_01090"
     CDS             complement(1308..1751)
                     /gene="rplM"
                     /locus_tag="VF874_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9174732.1"
                     /translation="MPTYTPKAGDTTRSWYVIDATDVVLGRLAVAAATLLRGKHKPTF
                     TPNVDGGDFVIVVNADKVAISGDKLQNKFAYRHSGFPGGLRARSIGDLMQKHPDRVVE
                     KAILGMLPKNRLGRQIKRKLRVYAGPDHPHKAQQPVPYEIKQVSQ"
     gene            complement(2060..2344)
                     /locus_tag="VF874_01095"
     CDS             complement(2060..2344)
                     /locus_tag="VF874_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEX9174733.1"
                     /translation="MSYNFAEIEFSVRQDIHSTSARLNSALDDLRAQITPLRQIWTRE
                     AADAYQLEQNQWDASAAALNDILFRLGNAVRDGADDVAATDRRAASAWGR"
BASE COUNT          325 a          747 c          822 g          451 t
ORIGIN      
        1 aactagccgg cgtcaggccc gacgtcggcg tcatcggcgc cgtcggctcc gctggctgcg
       61 cgccgttcgg ctggtcgacg gcgttctcgg cgttcggccc ggtcgccggc gcggtgttgg
      121 cgttcggcgt gttcgtcggc acgttgttcg cgttcggcga agtcaccggg ccgttctgcg
      181 cgatggtcac cacgccctga gcgctgtcgc aggtggtcgt gggtatctgt ccagcgtcca
      241 cctggttggc gagggccgtg atgtcgcgga cgttggtgtt ggtttcgttc gcggacattc
      301 cggtcgcgtt cggaccgtta gtggcggccc ctgggtttgc agctgtcccc gtgacaccgg
      361 ttccacagag aagtccggcg atctgcgatg caacgtccac gttgacgccc tgaaggaagt
      421 ccgcgttgcc cacaccgagg ttgacgagct gatcgtcgac gttggtcccg ggcgccgcgc
      481 tggcgatccc gatgccgccg gcgaacagca gcgacccacc gagtactgca ccagccccag
      541 ctcgcttgac aatcttgtgc atcaattact cctttttcgt ttttcgtcag gaagcagcta
      601 tccggccccg tcgtgctctg gtagccaccg gcggcagggt cggcctgcct ggcgtaagaa
      661 cgctggcacg aagaatcgcc ccctgctccc tcggttaggg gttcacgatc gagggactcg
      721 ccaaacctcg attccgcgaa aaggcgaaaa ttaagcaatc ggcttcagcg ccgggcgcgc
      781 agaacgcccg gtgactgaag ccagcgcgaa tcagcgcttc gagtactgcg gcgccttgcg
      841 ggccttcttc aggccgtact tcttgcgctc ggtggcccgc gggtcccggg tgaggaaacc
      901 ggccttcttc agcgccggcc ggtcctcggg ttgcaccagg atcagcgcgc gggcgatcgc
      961 caagcgcagc gcgccggcct ggccggacgg gccgccgccg tcgagatgcg cataaatgtc
     1021 gacgctgtcc acccggtcga cggtgactag cggcgccttg atcagctgct ggtgcacctt
     1081 gttcgggaag tagtcctcga gcgtgcggcc gtccaggtcg aacttgcccg tgccgggcac
     1141 caggcggacg cgaacgacgg cctccttgcg ccggccgacg gtctggatcg ggcggtcgat
     1201 gacgacgggc tcgcgggccg cgggggcctc cgtcgtgacc tcggtggtct cgatggtctc
     1261 ggtggtctcg gtggtctcgg tggtctcggt ggtctcggtg gtctcggtca ctggctcacc
     1321 tgcttgatct cgtacggcac cggctgctgg gccttgtgcg gatggtcggg tccggcgtag
     1381 acgcgcagct tgcgcttgat ctgacggccc aaccgattct tgggcagcat cccgaggatc
     1441 gccttctcga cgacgcggtc ggggtgcttc tgcatcaagt caccgatgga gcgagcccgc
     1501 aggccgccgg gaaaccccga gtggcggtag gcgaacttgt tctgcagctt gtcgccgctg
     1561 atggcgacct tgtcggcgtt gacgacgatg acgaaatcgc cgccgtcgac gttgggagtg
     1621 aacgtcggct tgtgcttacc gcgcagcagc gtggccgccg cgacggcaag gcggccgagc
     1681 acgacgtccg tggcgtcgat gacgtaccac gagcgcgtgg tgtcaccagc cttcggcgtg
     1741 tacgtgggca cagcgcttac ctctcttctt cgggtggatc ccggtgtgac ccgggtgccg
     1801 gtcaggcgtc tgcggcgggg tgtgctctcg gcgaccgacg ttgacccgag tcccttttgg
     1861 cgtaccgcac gccaacgcag cagcttacct gcggccgttc ccgcaggtca aaatgcgggc
     1921 tggtggcttc gaacgcgcgg tggctgcgag atttcggcca gattttcgca acagccgcac
     1981 gttcgaggca tctcgcctga cataactgtg cggtcccgac ggctcccctc cgtcgggacc
     2041 gcacagaagt cagcaggggc tagcggcccc aggcgctcgc cgcgcggcga tcggtggcgg
     2101 cgacgtcgtc ggcgccgtcg cgcaccgcgt tgccgagccg gaacaggatg tcgttcagcg
     2161 ccgcggcgga ggcgtcccat tggttctgtt cgagctgata ggcgtcggcc gcctcgcgcg
     2221 tccagatttg acgcagcgga gtgatctggg cgcgcaggtc gtcgagcgcg gagttgagac
     2281 gggccgacgt cgagtggatg tcttggcgta ccgagaactc gatttcggcg aagttgtaag
     2341 acagg
//
LOCUS       DASWBF010000038         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_200780,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000038 DASWBF010000000
VERSION     DASWBF010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200780"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2022
                     /gene="ponA2"
                     /locus_tag="VF874_01100"
     CDS             <1..2022
                     /gene="ponA2"
                     /locus_tag="VF874_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase/D,D-transpeptidase PonA2"
                     /protein_id="HEX9174734.1"
                     /translation="ERRAAVETTPARKLREIRMALTLDKTFSKPEILTRYLNLVFFGN
                     GAFGVQDAARTYFGIDASQLNWQQAALLAGLVQSTSALDPYVNPDKALQRRNVVLDTM
                     IENFPQHADALRAAKAEPLGVLAQPNQLPGGCIAAGNRAFFCDYVLDYLARAGISRDQ
                     VARGGYLIRTTLDPVVQNSVKQAVDKVASPTLDGVASVMSVIKPGKDSHRVTAMASNR
                     TYGLSSEAGETMQPQPFSLVGDGAGSIFKIFTTAAALEMGMGINAQLEVPGSFQAKGL
                     GSSDTPGCPKETWCVKNAGRYRGAMNVTDALATSPNTAFARLIQQVGVARAVDMSVRL
                     GLRSYGIPGTARAYDPDGNESLADLIKKQNMGSFTLGPFQLNALELSNVAATLASGGM
                     WCPPSPIDKIYDRKGNEVSVTTEACEQAVPEGLANTLANAMSKDDQGAGTAAASAGSV
                     GWSLPMSGKTGTTESHRSSGFVGFTNNYAAAVYIFDDSSTPSGICSFPLRKCYSGDLY
                     GGNEPARTWFEAMKPIATDFGEVKLPPTDPRYVDGAPGGRVPIVSGLNLDTARQRLRD
                     AGFQVADQPTPVNSTASYGTVVGTTPSGQTIPGSIVTINTSNGIAPPPPPPVEREPEG
                     PPPPPGEPPPPPPPPNVIEIPGLPPITLPAPEPPPPPAEPLPPPEPPPA"
     gene            complement(2031..>2211)
                     /locus_tag="VF874_01105"
     CDS             complement(2031..>2211)
                     /locus_tag="VF874_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174735.1"
                     /translation="VYPGTDAETRGIVVEDFGEMTPQAVDIGDSHFADPARRWAVLLE
                     TGSLVFIDTDQLLPE"
BASE COUNT          398 a          747 c          727 g          339 t
ORIGIN      
        1 gagcggcgcg ccgcggtgga gaccacgccg gcgcgcaagc tgcgtgagat ccgaatggcg
       61 ctcacgctgg acaagacgtt ctccaagccc gagatcctca cccgttacct caacctcgtc
      121 ttcttcggca acggcgcgtt cggggtgcag gacgccgcgc ggacgtactt cggcatcgac
      181 gcctcccagc tcaactggca gcaggccgcc ctgctggccg gcctggtcca gtcgacgagc
      241 gcactcgatc cctacgtcaa cccagacaag gcgctgcagc ggcgaaacgt ggtgctggac
      301 accatgatcg agaactttcc tcagcacgcc gacgcgctgc gcgccgcgaa agcagaaccg
      361 ctgggcgtgc tggcccaacc caaccagctt cccgggggct gcatcgcggc aggcaatcgg
      421 gcgttcttct gcgactacgt gctggactac ctggcgcgtg ccggcatcag cagggatcaa
      481 gtggcccgcg gcggttatct gattcgcacc accctcgacc cggttgtcca gaattcggtc
      541 aagcaggccg tcgacaaggt cgccagcccg acgctggacg gtgtcgcgag cgtgatgtcg
      601 gtgatcaagc ccggcaagga ctcgcatcgg gtgacggcga tggccagcaa ccggacctac
      661 ggcttgagct cggaagccgg cgagacgatg cagccgcagc cgttctcgtt ggtcggtgac
      721 ggcgcggggt cgatcttcaa gatcttcacc accgccgccg cgctggagat gggcatgggg
      781 atcaacgctc agttggaggt gccgggctcc ttccaggcca agggcctggg cagcagcgac
      841 acaccgggct gccccaagga aacctggtgc gtgaaaaacg cgggacgata ccggggtgcg
      901 atgaacgtca ccgatgcgct ggccacctcc ccgaacaccg cgttcgccag gctgattcaa
      961 caggtcgggg tggcgcgcgc cgtggacatg tcggtccggc tggggttgcg gtcgtacggg
     1021 attccgggaa cagcgcgggc gtatgacccg gacggcaacg aaagcctggc cgatttgatc
     1081 aagaagcaga acatgggctc gttcacgctg ggccccttcc agctgaacgc acttgagctg
     1141 tccaacgtgg cggcgacgct ggcgtcgggc ggtatgtggt gcccacccag cccgatcgac
     1201 aagatctacg accgcaaggg caacgaggtg tcggtgacca ccgaggcctg tgaacaagcg
     1261 gtgccggagg gcctggcgaa cacgctcgcg aacgcgatga gcaaggacga ccaaggcgcc
     1321 ggcaccgccg ccgcctctgc gggctcggtg ggctggtctc tgccgatgtc cggcaagacc
     1381 ggcaccaccg agtcccatcg gtcgtcggga ttcgtcggct tcaccaacaa ctacgcggcg
     1441 gcggtttaca ttttcgacga ctccagcaca ccgtctggca tctgttcgtt tccgcttcgc
     1501 aagtgctaca gcggtgactt gtacggcggc aacgaacccg cgcgtacctg gttcgaggcg
     1561 atgaagccga tcgcgaccga cttcggtgag gtgaagctgc cacccaccga tccgcggtat
     1621 gtcgacggcg cgccgggtgg tcgggtgccg attgtgtcgg gactgaattt ggacaccgcg
     1681 cgacagcgct tgagggacgc gggttttcag gttgccgatc agccgacacc ggtgaacagc
     1741 accgcctcct acggcaccgt cgtcggcacc acgcccagcg gccagacgat ccccgggtcg
     1801 atcgtcacga tcaacaccag caacgggatt gcgccgccac cgccacctcc ggtggagcgg
     1861 gagccggagg gtccgccccc accgcccgga gagccgccgc caccaccgcc gccgccgaac
     1921 gtcatcgaaa ttccggggct gccgccgatc accctgcccg cgccagagcc tccaccaccg
     1981 ccggccgaac cactaccacc gcccgaacca ccgcccgcct agagcgagaa ttactcgggc
     2041 aggagttggt cggtgtcgat gaacaccagg ctgccggttt cgagcagtac cgcccaccgt
     2101 cgggccgggt cggcgaaatg gctgtcgccg atgtcgacgg cttgaggcgt catctcgccg
     2161 aagtcctcga cgacgatgcc tcgggtttca gcgtccgtgc cggggtagac a
//
LOCUS       DASWBF010000039         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_661899,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000039 DASWBF010000000
VERSION     DASWBF010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661899"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1724
                     /gene="kdpB"
                     /locus_tag="VF874_01110"
     CDS             <1..1724
                     /gene="kdpB"
                     /locus_tag="VF874_01110"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX9174736.1"
                     /translation="GDIVVIEAGQLIPGDGDVVDGIASVDESAITGESAPVIRESGGD
                     RSAVTGGTTVLSDRIVVRITQKPGESFIDRMIALVEGANRQKTPNEIALNILLAALTI
                     IFVFAVATLQPLAIYSKMNNPGVSDTLALNSHGVTGIVMVSLLVCLIPTTIGALLSAI
                     GIAGMDRLVQRNVLAMSGRAVEAAGDVNTLLLDKTGTITLGNRQAAAFLPLPGVSPAA
                     LADAAQLSSLADETPEGRSVVVFAKQKYGLRARTPGELAHAQWVQFTAATRMSGVNLD
                     GHLLRKGAASSVAEWVRAEGGEVPTELGVIVDGVSAGGGTPLVVGHAVDGKASVLGVI
                     HLKDVVKQGMRERFDEMRRMGIRTVMITGDNPLTAKAIADEAGVDDFLAEATPEDKMA
                     LIKREQEGGKLVAMTGDGTNDAPALAQADVGVAMNTGTSAAKEAGNMVDLDSDPTKLI
                     EIVEIGKQLLITRGALTTFSIANDIAKYFAIIPAMFVALFPGLDLVNIMRLHSPQSAI
                     LSAVIFNAVVIIALIPLSLRGVRYTPSSASKLLSRNLYIYGLGGIIAPFIGIKLIDLV
                     IQFFPGM"
     gene            1726..2634
                     /locus_tag="VF874_01115"
     CDS             1726..2634
                     /locus_tag="VF874_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HEX9174737.1"
                     /translation="MNTSNFVRQHWAALRALLVLTVIVGIAYPVFIWLVAQLPGLRDN
                     ANGSIVEVDGKPVGSSLIGQLFTDSDGNPLPQYFQSRPSAAGDGYDPLATSASNLGPE
                     SIVDTPGDPSLPQDDNGFKASLLTQVCSRSLAAGELEGVDGSRPFCTSDGVGAVLSVI
                     GGRDARGNVVKPTRVVSVNQPCETTPTPFLNIYEGVRVECAKFGEDYSIGQIVPIRGA
                     APADPAVSADAVTASGSGLDPHISVAYAELQIPRVANARGITQDQVRQAVHDNQDGRV
                     LGFLGEARVNVLELNLELDQKFGVKS"
BASE COUNT          489 a          882 c          911 g          467 t
ORIGIN      
        1 agggcgacat cgtggtgatc gaggcaggcc agctcatccc tggcgacggt gacgtagtgg
       61 acggcattgc gtcggtggac gagtcggcaa tcaccggcga gtcggcgccg gtgatccgcg
      121 agtccggcgg cgaccgctct gcggtaaccg gcggcaccac ggtgctgtcg gatcgcatcg
      181 tcgtgcggat cacccagaag ccaggtgaga gcttcatcga ccggatgatc gcgctcgtcg
      241 agggcgccaa tcgacagaag accccgaacg agatcgcgct gaacatcctg ctcgccgcgt
      301 tgacgatcat cttcgtgttc gccgtcgcaa cactgcagcc gcttgcgatc tactccaaga
      361 tgaacaaccc gggcgtttcg gataccttgg cgctcaacag tcatggcgtg accgggatcg
      421 tgatggtgtc acttcttgtg tgcttgatcc cgaccacaat cggcgcgctg ctgtccgcga
      481 taggcatcgc cggcatggac cggctcgtgc agcgcaacgt actggcgatg tcgggtcgcg
      541 ccgtcgaggc cgcaggcgac gtgaacacgc tgctgctcga caagaccggc acgatcacgt
      601 tgggcaatcg ccaggccgcg gcgttcctcc cgctgcccgg ggtctccccg gcggccttgg
      661 cggatgcggc gcagctgtcg agcctcgccg acgaaacgcc cgagggccgt tcggttgtcg
      721 tgttcgccaa gcagaagtac gggctgcggg cgcgcacacc gggtgagttg gcgcacgcac
      781 agtgggttca gttcaccgcc gcgacccgga tgtcgggcgt caacctcgac ggacacctgc
      841 tgcgcaaggg tgcggccagt tcggtcgcgg aatgggtacg agccgagggc ggcgaggtgc
      901 ccaccgagct cggcgtcatc gtcgacggcg tttcggcggg tggcggcacg ccgctggtgg
      961 tcggacacgc cgtcgacggc aaggcgtcgg tgctgggcgt gatccacctc aaggacgtcg
     1021 tcaagcaggg catgcgggag cgtttcgacg agatgcgccg gatgggcatc cggaccgtga
     1081 tgatcaccgg tgacaacccg ttgaccgcca aggcgattgc ggatgaagcc ggcgtcgacg
     1141 acttccttgc ggaagctacc cccgaagaca agatggcgct gatcaagcgg gagcaggagg
     1201 gcggcaagct cgtcgcgatg accggcgacg gcaccaacga cgccccggcg ctcgcccagg
     1261 ccgacgtcgg tgtggcgatg aacaccggca cgtcggcggc caaagaggcc ggcaacatgg
     1321 tcgacctcga ctcggatccg accaagctga tcgagatcgt cgagatcggc aagcagctgt
     1381 tgatcacccg cggagcgctg accacgttct cgatcgccaa tgacatagcg aagtacttcg
     1441 cgatcatccc ggcgatgttc gtggcattgt tccccgggct ggatctggtg aacatcatgc
     1501 ggctgcacag ccctcagtcg gcgatcctgt cggcggtgat cttcaacgcg gtggtcatca
     1561 tcgcgctgat tccgctgtcg ctgaggggcg ttcggtatac cccgagcagc gcatcgaagc
     1621 tgctgtcacg aaatctgtac atctacgggc ttggcggcat catcgccccg ttcatcggaa
     1681 tcaagctcat cgatctcgtc atccaattct tcccgggaat gtgacatgaa tacctccaat
     1741 ttcgttcgcc agcactgggc cgcgctgcgc gcgctgctcg tgctgaccgt catcgtcggc
     1801 atcgcctacc cggtcttcat atggctggtg gcgcagctgc ccggcttgcg ggacaacgcc
     1861 aacgggtcga tcgtcgaggt cgacggtaag cctgtgggca gcagcctgat tggccaactg
     1921 ttcaccgact ccgatggcaa cccgttgccg cagtacttcc agagccgccc gtcggccgcc
     1981 ggcgacggct acgacccgct cgcgacgagc gcgagcaacc tggggccgga aagcattgtc
     2041 gacacccccg gtgatccgtc gctgcctcag gacgacaacg gattcaaggc gagtctgctc
     2101 acgcaggtgt gttcgcgcag ccttgcggcc ggcgagctcg aaggcgtcga cggctcacga
     2161 cccttctgca cctccgacgg cgtcggcgcg gtgctgtcgg tgatcggggg ccgggacgcg
     2221 cgcggcaacg tcgtcaagcc cacaagagtg gtcagcgtca accaaccgtg cgaaacgact
     2281 ccgaccccct ttctgaacat ctacgaaggt gtgcgggtgg aatgcgccaa gttcggcgag
     2341 gactactcga tcggccagat cgtgccgatc cgcggcgccg ccccagctga tcccgcggtg
     2401 tctgccgatg ccgtcacggc gagcggcagc ggcctcgatc cgcatatctc tgtggcctac
     2461 gcggaactcc aaatcccccg tgtcgcgaac gcgcgaggca tcacccaaga tcaggtgcgc
     2521 caggcagtgc atgacaacca ggacggccgg gtgctcggct tcttgggcga ggcgcgggtc
     2581 aacgtgctgg agctcaatct ggaactggac cagaagttcg gtgtgaagag ctgatcaacg
     2641 cggtggatga tgggcacgtg accaccaccg acgagccggt catccatcgg cccgactaca
     2701 aacgcaaacg cggggagctg cgcatttacc tcggcgcagc tcccggcgt
//
LOCUS       DASWBF010000040         9136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_69036, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000040 DASWBF010000000
VERSION     DASWBF010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69036"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..335)
                     /locus_tag="VF874_01120"
                     /pseudo
     CDS             complement(1..335)
                     /locus_tag="VF874_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891174.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            381..2768
                     /locus_tag="VF874_01125"
     CDS             381..2768
                     /locus_tag="VF874_01125"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9174738.1"
                     /translation="MEFVLLLAGVRVLDLADGDGEAVTRLLADLGADVLKIEPPRGSA
                     ARRAEPSVAGVSIPFALHNANKRSAVLDPADDDDRRRFLDLVAGADILVDDGIPGAAA
                     SFGAGCEQLTRRFGHLIGLSVTNFGSSGPYASRQATDPVLYAMSTALSRSGPPTGTPV
                     LPPDGIASATAAAQAAWAALAAYYRRLRCGAGDYIDFSRFEAVVQALDPPFGAMGQGA
                     LAQRRFDAWRGRPRNQDVYPTFSCADGYVRSCVMSPRQWHAMRAWLGEPEQFADPRYE
                     TIGARLSATREINAMVAQLFADQSMDELVAAGQSYGVPIAPVLTPAEALSSPHFEAVG
                     ALTTRELPPGVTVTAPAGPFVVDGRRAGLRRPAPPVGADAATWLGPAVPAPAAGSADR
                     RPFDGLRILDLGIIVAGGELGRLFADLGAEVIKMENAAYPDGLRQTRASQPMSEAFAW
                     AHRNKYGLGLDLRKPEGAAIFSRLVAESDAVFANFKPGTLASLGFPYGRLRAINPRIV
                     LAESSAFGDTGPWSWHMGYGPLVRAATGVTRLWTSGRIGDGFFDAVTVFPDHVVARVV
                     AIAALAAVIRRDRTGQPAHVHISQAEVAINQLDTTYVADEARAAGLPVSGDPVTHGVY
                     PCAGDDEWCVISVRDDTDWNALTTAMGREGLDRTQCEAEIASWTRTLDKEAVARILQQ
                     AGVPAGPMNRPADVLADPQIQHRKLFADMAHPLFDFLLPTETDPAPYRDIPPAELRPA
                     PMPGEHSREVCQKVLGLDPAEIDKLIADGALFTSPISARAQGKVGGCPSIPEHRC"
     gene            2738..4213
                     /locus_tag="VF874_01130"
     CDS             2738..4213
                     /locus_tag="VF874_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX9174739.1"
                     /translation="MPVDPRTPVLIGSGQINQYDDDEAPDPVGLIAEAAREAADAHVL
                     EAIDAIRIVRMLSWRYRDPGLLLGQQIGATRFASRYSGVGGNVPQSLVNQACLDIQRG
                     RADVVLIGGAEKWRTRTRLRAEDRKPDWPPQDDAVPEAPGNDGDVPMAGPAEMRIQLF
                     APAHVYPMFEQALRIAAGESSDEHRRRIGELWSQFSAVAEGNPYAWSRQRLSAQEIWQ
                     PGPTNRMISWPYPKLMNSNNMVEQAAVIILASAEAATRLKIPSEGWVFPYSGTDSHDT
                     YALGERADFHRSPAIRIAGRRALELAGCGIGDVDFVDVYSCFPSAVQVAATELGLPLA
                     DPQRPLTVTGGLTFAGGPWNNYVTHSIGTMAERLVEKPDGLGLITANGGNLTKHSIGI
                     YGTRPPSTEFRWEDVQSEVNREPTRTALEHWDGIGSVEAWTAPFDRDGNPEKAFVAVR
                     TPGDARTLAVMTASSDVDASVTEDIAGAKVQVHADGTAELR"
     gene            complement(4214..4795)
                     /locus_tag="VF874_01135"
     CDS             complement(4214..4795)
                     /locus_tag="VF874_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174740.1"
                     /translation="MNAARVAVCAFTVVVTAGCADAVVVNTGESPPRTETTVLTSPAG
                     RSDPNLVDASDYYVTTDGLKGYYFTIPSGKWNCAILPRSKAGCQAAAGTQGMGIPGEP
                     DTVQTADGEAVAPNAIAVGDEGDPGFIWVARPGFSVTSGKALVLDFTKTLAAAGFRCN
                     VQEAGVSCLSEGTKKGFTFSASGYSPQYTAVPG"
     gene            complement(4840..5565)
                     /locus_tag="VF874_01140"
     CDS             complement(4840..5565)
                     /locus_tag="VF874_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9174741.1"
                     /translation="MIRIGTSGWSYDHWVGVLYPERTPVSARLARYVQEFTTVELNAS
                     FYRWPKDSTFAGWRQRLPDGFTMSVKAHRGLTHFRRLKSPEPWVERFERCWHALGNRR
                     EMLLVQLHPEQQRDDERLDYFLGCMPDWIRVAVELRHPSWDDPAVYALLEHRRAAYVV
                     MSGARLPCVLRATTDVVYVRMHGPDSESLYAGSYSDGDLRWWADRIAQWDAQGRDVLV
                     YFNNDGHGYAVRNARMLRGLLKV"
     gene            complement(5562..6257)
                     /locus_tag="VF874_01145"
     CDS             complement(5562..6257)
                     /locus_tag="VF874_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9174742.1"
                     /translation="MTNADGVGAMSRLREEQTTENRLERRKMRTRAALIRAAQSFIAS
                     GTLNVPVLEITQAADVGMGSFYNHFDSKEQLFQAAINEVLDAHGTLLDRLTDEDEDPA
                     ETFARSFRLTGRLFRRRPDMSRVLLNSGLVLISSDRGVAPRALRDIEAATRAGRFSVD
                     DPTLALAVAAGALMGLGQLLYDQPERDDAEAADRVTRDVLIMFGVAADEAAVICGRPL
                     PELDELTEPDSAA"
     gene            6365..7498
                     /locus_tag="VF874_01150"
     CDS             6365..7498
                     /locus_tag="VF874_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005257714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9174743.1"
                     /translation="MTAHVDAHKDLHSEQGGLPGEHPGRSRNPVIKVRDLAWLEFEKP
                     DLVRAEAFARAFGFTTVVHTSDELHVRGTDSGSPCLVFRRGTSSRFVGPAFAAQDNTD
                     VGRLAAATGARITPLPDALGGVAVTLTDPNGGPVRVVAGTHPLAELPGQRPQTLNFGH
                     DLRRTNATQRPPREPARVQRLGHVVLQRTKYIQTLNWYLDHFGLIVSDFLYYPGQRDR
                     GPVMSFIRCDRGSTPTDHHTLAMVLGPSNRYVHSAYQVCDLDAIAAGGEYLRELGYRR
                     SWGIGRHIQGSQIFDYWRDPDGFMVEHFSDGDMFDATLEPGWAPMTASGLAQWGPPVT
                     KDFLGIAPNRESLKELRSMLTAIRDDNEFDMTRLRGLLKVTRT"
     gene            7495..8433
                     /locus_tag="VF874_01155"
     CDS             7495..8433
                     /locus_tag="VF874_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9174744.1"
                     /translation="MSISVFRTADAWWAGTPDGVAKVDTRTTTTAQLLADRSAIERAA
                     ASTDTVPANSLALTSPVTAPCRVVAQMTNFASHVKDAGMNPKTVPLTFFRKTSGSING
                     PFDDIVKPGHVRLLDYEVEIGLVIGRDVPVGTHVSPDNVADYVCGLVVTNDVSARDIQ
                     LAKTQFYESKSYPTFTPVGPALVLLDADELSRFGELRMQLRVNGELRQDATVDGDMIY
                     RPAEALAALARFQQLAAGDLVMTGTPVGTALSAPPKPVQKIASLVPEHVKWRMFFSAQ
                     AKNAKYLHDGDVVETTVATDDGKIDLGTQRNVVRYA"
     gene            8430..>9136
                     /locus_tag="VF874_01160"
     CDS             8430..>9136
                     /locus_tag="VF874_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9174745.1"
                     /translation="MNSNVIPVVIVGAGPTGVTAATLLAQYGVECLVLDRWQSVYPQP
                     RAVHLDDEICRIIGRLGIADEFATISRPALGLQLRDRAMRVLAQFQRDSAGGAHGYPQ
                     ANMFDQPELETLLRANLKRYPHATLRANSEVTDVAATGADRVRVTFTDRLSGQKHSVD
                     ADYVLGCDGANSVVRAAIGASMRDMRFHQRWLVVDVASAEDLHQWDGVHQVCDPHRAA
                     TYMRIGDTRYRWEFQLLP"
BASE COUNT         1548 a         3090 c         3027 g         1471 t
ORIGIN      
        1 ccgattccgg agtacacctc gccgtaggtg ccctcgaggt acgcgttggc cacccgtcgg
       61 gtccgccgtg gccggggccc gcaatgtaga cgacatcgag atcgcgggcc cgaatgatcc
      121 ggttcaggtg cgcgtaaatc agattcaaac cgggggtggt gccccagtgg ccgagcagcc
      181 gcggcttgac gtgcgccacc tcggacagcg gctcagccag cagcgggttg tccgacaggt
      241 ggatctgtcc ccattgacag gtaattggcg gcccgccagt acgcattgat cagatcgagc
      301 tcgtcgtcgg agaggagcag gtttggcgcg gtcacgacca caggcgtacc cgaatcgggc
      361 tacattacgc tcacttcgtg ctggaattcg tgttgctgct cgccggggtg cgcgtcctcg
      421 acctggccga cggcgacggc gaggcggtca cccgtctgct cgctgacctc ggcgccgacg
      481 tgctgaagat cgagccaccc cgcggcagcg cggcccgtcg tgcggagccg agcgtggccg
      541 gcgtgagcat cccgttcgcc ctgcacaacg cgaacaagcg cagcgcagtg ctggatcctg
      601 ccgacgatga cgaccgccgc cgattcctgg atctcgtcgc gggcgccgac attttggtgg
      661 acgacggaat ccccggcgcg gcagcatcct tcggcgccgg ctgcgagcag ctcacccggc
      721 ggttcggcca cctgatcggg ttgtcggtta ccaacttcgg ctcgtcgggg ccgtatgcgt
      781 cgcggcaggc cacggatcct gtgctctatg cgatgtccac ggcgctgtcg cggtccggcc
      841 caccgaccgg gacgcctgtg ctgccacccg acgggatagc gtcggccacc gccgcggcgc
      901 aggccgcatg ggccgcactg gccgcctact atcgccgatt acgttgcggt gcaggggatt
      961 acatcgactt ctcccggttc gaagccgtcg tgcaggcgct ggatccgccg ttcggcgcga
     1021 tgggccaggg tgcgttggcg cagcggcgct tcgacgcctg gcgcggccgc ccccggaacc
     1081 aggacgtgta tccgacgttt tcgtgcgcag acggctatgt ccgcagctgc gtgatgtcgc
     1141 cgcggcagtg gcatgccatg cgggcctggc tcggagagcc cgagcagttc gccgacccga
     1201 ggtacgagac catcggcgcg cggttgtcgg caacgcgtga aatcaatgcg atggtcgcgc
     1261 agttgttcgc cgatcagtcg atggacgagt tggtggcggc cggccagagc tatggggtgc
     1321 cgatcgcgcc cgtgctgaca ccggcagagg cgttatcgtc gccgcatttc gaggcggtgg
     1381 gagcgctgac gacgcgagag ctcccacccg gtgtgacggt gacggcgccc gccggaccgt
     1441 tcgtcgtcga cggccgtcgg gcagggctgc gtcggcccgc gccgccggta ggcgcggacg
     1501 cggcgacgtg gcttggtcca gccgtgccgg caccggcggc cggtagcgcc gatcgccggc
     1561 cgttcgacgg gctccgcatt ctcgacctcg ggatcatcgt cgccggggga gagttgggcc
     1621 gcttgttcgc cgacctcggt gccgaggtga tcaagatgga aaacgccgcc tatcccgacg
     1681 gactacgcca aaccagggct tcccagccga tgagcgaggc gttcgcgtgg gcgcaccgca
     1741 acaagtacgg gcttggtcta gacctgcgaa aaccggaagg cgcagcgatt ttcagccgcc
     1801 tggtagccga gtcggacgcg gtgttcgcca actttaagcc aggaacgctt gcctcccttg
     1861 gttttccgta cggcaggctc cgcgcgatca acccgcgcat cgtgctggcc gagagcagcg
     1921 cgttcggtga caccggcccg tggagctggc acatgggcta tggcccgctg gtgcgcgcgg
     1981 ccaccggcgt cacgcggctg tggacgtcgg ggaggatcgg tgatgggttc ttcgacgcgg
     2041 tcacggtgtt tcctgatcat gtcgtcgcac gcgtcgtcgc gatcgcggcg ctggcagccg
     2101 tgatccgccg cgaccgcacc ggacagccgg cgcacgtgca tatctcacaa gcggaggtcg
     2161 cgatcaacca gctggacacc acctacgtcg ccgacgaggc ccgagccgca gggctacccg
     2221 tttccggcga cccggtgacg cacggcgtgt acccctgcgc cggtgacgac gaatggtgcg
     2281 tcatctccgt ccgcgacgac accgactgga acgcgctcac cacggcgatg ggccgcgagg
     2341 gcctggatcg cacgcaatgt gaggccgaaa tcgcctcgtg gacgagaact ctcgacaagg
     2401 aagccgtagc ccgcattctg cagcaggccg gcgtgcccgc cgggccgatg aaccgccccg
     2461 ccgacgtcct cgccgacccg cagatccaac accgaaagtt gttcgccgac atggcgcatc
     2521 cgctgttcga cttcctcctg cccaccgaga ccgacccagc gccgtaccgc gacatcccgc
     2581 ccgcggagct gcggcccgcg ccgatgcccg gcgaacacag ccgcgaggtg tgtcagaagg
     2641 tgcttggcct ggaccccgca gagatcgaca agctgatcgc cgacggtgcg ctgttcacgt
     2701 ctcccatttc tgcgcgagcg caaggaaagg ttggtggatg cccgtcgatc ccagaacacc
     2761 ggtgctgatc ggatccggac aaatcaacca gtacgacgac gacgaggcgc cggatcccgt
     2821 cggcttgatt gccgaggcgg cgcgcgaggc cgccgatgcg catgtgctcg aagccatcga
     2881 cgcaatccgc atcgtgagga tgctgtcgtg gcgttaccgc gatcccgggc tgctgctcgg
     2941 ccagcagatc ggtgccaccc gcttcgcatc gcgctacagc ggcgtcggcg gcaacgtgcc
     3001 acaatctctg gtcaatcagg cctgtctgga catccagcgc gggcgggccg acgtcgtgtt
     3061 gatcggtggt gccgaaaagt ggcgcacccg aacgcgattg cgagccgaag acaggaaacc
     3121 ggactggccg ccgcaggacg acgccgtgcc ggaagccccg ggaaatgacg gcgacgttcc
     3181 gatggccggt ccggccgaaa tgcgcatcca actcttcgca ccggcgcacg tgtacccgat
     3241 gttcgagcag gcgctgcgga tcgcggccgg cgagtcgtcg gatgaacacc gccgccgaat
     3301 cggcgagttg tggtcgcagt tcagcgctgt cgccgaaggt aacccgtacg cgtggagccg
     3361 acaacgactt tcggcacaag agatctggca gccggggccg acgaaccgga tgatcagttg
     3421 gccgtacccg aagctgatga actccaacaa catggtcgag caggcagccg tcatcattct
     3481 ggcgtccgcc gaggcggcca cccgactcaa aatcccgtcg gaggggtggg tgttccctta
     3541 ctccggaacg gattcgcatg acacgtacgc cctcggcgag cgggctgact tccaccgttc
     3601 accggcgatc aggatcgccg gccgccgcgc gctggaactg gccggatgcg gcatcggtga
     3661 cgtcgatttc gtcgacgtct actcgtgttt tccatcggct gtccaggtcg ccgcaacaga
     3721 attgggtctt ccactcgccg atccgcagcg gccgctcaca gtcaccggcg ggctgacatt
     3781 cgccggcggc ccgtggaaca actatgtgac tcactcgatc ggcacgatgg ccgagcggct
     3841 cgtcgagaag ccggacggcc tggggttgat caccgccaac ggcggaaatc tcactaaaca
     3901 cagcatcggc atctacggca cccgtccacc gagcaccgaa ttccgttggg aggacgtgca
     3961 atccgaggtc aaccgcgagc cgaccaggac cgcattggag cactgggacg ggatcggcag
     4021 cgtggaggca tggacggccc ccttcgatcg tgacggcaac ccggagaagg cgttcgttgc
     4081 ggtgcgcacc cccggcgacg cgcgcacgct ggcggtgatg acggcatcct cggatgtgga
     4141 cgcctcggtc accgaggaca tcgcaggggc caaggtgcag gttcacgccg acgggaccgc
     4201 cgagctacgc tgatcagccc ggcactgcgg tgtattgcgg cgagtaaccg ctggcggaga
     4261 acgtgaaccc ctttttcgtg ccctcgctca ggcacgacac cccggcctcc tgaacattgc
     4321 agcgaaagcc cgccgccgcc aacgtcttgg tgaagtccaa caccagcgcc ttgccggacg
     4381 ttaccgagaa tcccggccgg gcaacccata tgaagccggg atccccttcg tcgccgaccg
     4441 cgatcgcgtt cggggcgacc gcctcaccgt cggcggtctg aaccgtgtcg ggttctccgg
     4501 gaatgcccat gccctgcgtg ccggccgcgg cctggcagcc cgccttcgag cgcgggagaa
     4561 tcgcacagtt ccactttccg ctcgggatcg tgaagtagta gcctttcagg ccgtcggtgg
     4621 tgacgtaata gtcagacgca tcaaccagat tggggtcgga gcgaccggct ggcgaggtca
     4681 agaccgtggt ctcggtgcgc ggcgggctct ctccggtgtt caccacgacg gcatcagcgc
     4741 agccagcggt cactacgacg gtgaaagcgc acaccgccac ccgtgctgcg ttcatgccac
     4801 catcctttcg gcggcgacag tacaggttcg agcctgacgt cataccttca gcagcccccg
     4861 caacatccgc gcgttgcgca cggcgtagcc gtggccgtcg ttgttgaagt acacgagcac
     4921 atcgcgaccc tgtgcgtccc actgcgcaat gcggtccgcc caccatcgca ggtccccgtc
     4981 ggaatacgaa ccggcataaa gtgattcgct gtccggaccg tgcattcgca cgtacaccac
     5041 gtcggtcgtc gcgcgcagca cacacggcag ccgcgcgccg ctcatcacga cgtatgccgc
     5101 gcgacgatgc tcgagcagcg catagacagc cgggtcatcc caggaggggt ggcgcagctc
     5161 gacggccacc cggatccagt ccggcataca gcccaaaaag tagtcgagtc gctcgtcgtc
     5221 gcgttgctgt tctgggtgca gttgcaccag cagcatttcc cgccgattgc ccagcgcgtg
     5281 ccagcagcgc tcgaaccgct caacccacgg ttcgggggac ttcaggcgac ggaaatgggt
     5341 cagtccccga tgggccttca ccgacatcgt gaacccgtcg ggtaatcgct gccgccagcc
     5401 tgcgaacgtc gagtccttcg gccaccggta gaagctcgcg ttgagttcga ccgtggtgaa
     5461 ctcctgcacg tagcgggcca accgggcgga taccggcgtc cgctccgggt acagcacgcc
     5521 gacccagtgg tcgtaggacc agcccgacgt gccgatgcgg atcacgcggc ggagtcaggc
     5581 tcggtcagct cgtcgagctc gggcaggggc cggccacaaa tcacggcggc ctcgtcggca
     5641 gccacgccga acatgatcag cacgtctcgg gtgacccggt cggcggcctc ggcgtcgtcg
     5701 cgctcgggct ggtcgtacag cagctggccc agtcccatca acgcgccggc ggcgaccgcc
     5761 aatgcgagcg tggggtcgtc gacggaaaac cggcctgcac gtgtcgcggc ctcgatgtcg
     5821 cgcaaggcgc gcggtgcgac cccgcggtcc gacgagatca aaaccagccc gctgttcaac
     5881 agcacccggc tcatgtccgg gcggcgccgg aacagccgtc cggtcaaccg gaaactgcgg
     5941 gcgaacgtct ccgcggggtc ctcgtcctcg tcggtcagcc ggtcgagcag cgtgccgtgg
     6001 gcgtcgagca cctcgttgat cgcggcttgg aaaagctgct ccttgctgtc gaagtggttg
     6061 tagaacgacc ccatgccgac gtcggcggcc tgggtgatct caaggacggg cacgttcagc
     6121 gtccccgacg cgatgaagct ctgggccgcc cggatcagcg ccgcgcgggt gcgcatcttg
     6181 cgccgttcca ggcggttctc ggtcgtctgc tcttcgcgca agcggctcat cgcccccacc
     6241 ccgtccgcat ttgtcatggg gcagatgtta gcagaaatcc tcaatcttga tgatttcgtc
     6301 agaacgactt gactgacgag cccttcgtaa gtgacgatat cgtcagtcaa gtgaggagag
     6361 caccgtgaca gcccacgtcg acgcccacaa agacctgcac agcgagcagg gcggcctgcc
     6421 cggcgagcac ccgggacgtt cccgcaatcc ggtcatcaag gtgcgcgacc tggcgtggct
     6481 ggagttcgag aagcccgatc tggtccgtgc cgaagccttc gcgagagcgt tcggcttcac
     6541 caccgtcgtg cacacctccg acgaattgca cgtgcgcgga accgattccg ggtcgccgtg
     6601 cctggtcttc cggcgcggga cgagttcccg gttcgtcggg cccgcgttcg ccgcgcagga
     6661 caacaccgac gtcggacggc tggccgccgc gacgggcgcg agaatcacgc ctctgcccga
     6721 cgcgctcggc ggcgtggccg tgaccctgac cgacccgaac ggcggtccgg tccgtgtcgt
     6781 ggccggcacc cacccgctcg ccgagttgcc gggtcagagg ccgcagacgt tgaacttcgg
     6841 gcacgaccta cggcgcacca atgccacgca acggccgccg cgtgagccgg cccgcgtgca
     6901 gcggttgggg catgtcgtgc tgcagaggac caaatacatc cagacgctga actggtacct
     6961 cgatcacttc gggctgatcg tcagcgactt cctgtactac ccgggccagc gcgaccgagg
     7021 gccggtaatg agcttcatcc gctgcgaccg cggatcgacg cccaccgacc atcacacgct
     7081 cgcgatggtg ttgggcccgt cgaaccgcta cgtgcactcc gcctaccagg tgtgcgatct
     7141 cgatgcgatc gcggcgggtg gggagtacct tcgcgagctc ggctaccgcc ggtcgtgggg
     7201 gatcggcagg cacatccagg gcagccagat cttcgactac tggcgcgatc ccgacgggtt
     7261 catggtcgag catttcagcg acggcgacat gttcgacgcg acgctggagc caggctgggc
     7321 tccgatgacc gcgtcgggtc tcgcccagtg ggggccgccg gtgacaaagg acttcctggg
     7381 catcgcgccc aatcgggaat ccctgaagga gttgcggtcg atgctgaccg cgatccggga
     7441 cgacaacgaa ttcgacatga cccgcctgcg cggcctcctg aaagtgacac gcacatgagc
     7501 atttccgtct tccgcaccgc tgatgcgtgg tgggccggca cccccgacgg cgtcgccaag
     7561 gtcgacaccc gcacgacgac tacggcccag ttgctggctg accgatcggc gatcgagcga
     7621 gctgccgcca gcaccgacac ggtaccggcg aacagccttg cgctgacctc gcccgtcacc
     7681 gccccgtgcc gggtcgtggc ccagatgacc aacttcgcgt cgcacgtcaa ggacgccggt
     7741 atgaacccga agacggtgcc gctcacgttc tttcgcaaaa cgtcgggttc gatcaacggg
     7801 ccgttcgacg acatcgtcaa gccgggccat gtccgcctgc tcgactacga ggtggaaatc
     7861 ggcctggtga tcggccgtga cgtgcccgtc ggcacgcacg tttcccccga caacgtcgca
     7921 gactacgtct gcgggctggt ggtcaccaac gacgtgtcgg cgcgcgacat ccagctggcg
     7981 aaaacccagt tctacgagtc aaagtcgtat ccgacgttca cgcccgtcgg gccggcgctg
     8041 gtactgctgg atgccgatga gctcagtcgc ttcggagagc tgcgcatgca actgcgcgtg
     8101 aacggcgaac tgcgccaaga cgccacggtc gacggcgaca tgatctaccg gcccgccgag
     8161 gcgcttgccg cgctcgcccg gtttcagcag ctggctgcgg gtgacctcgt gatgaccggc
     8221 acgcccgtcg gcaccgcact gtccgcgccg cccaaaccgg tgcagaagat cgcgtcactc
     8281 gtgcccgagc acgtcaagtg gaggatgttc ttctcggcgc aagccaagaa cgccaagtac
     8341 ttacacgacg gggacgtcgt cgaaaccacc gtcgcaaccg acgacggcaa gatcgacctc
     8401 ggcacacagc gcaacgtggt caggtacgca tgaactcgaa cgtcatcccg gtggtgatcg
     8461 tcggtgccgg gccgaccggg gtgacggccg cgacgttgtt ggctcagtac ggcgtcgagt
     8521 gtctcgtgct cgatcgctgg cagagcgtgt acccgcaacc gcgggccgtg cacctcgacg
     8581 acgagatctg ccgcatcatc ggccggctcg gcatcgccga cgagttcgcc acgatctctc
     8641 gcccggcgct aggcctgcag ttgcgtgacc gtgcgatgcg ggtgctcgcg cagttccagc
     8701 gggattcggc cggtggcgcg catggctacc cgcaagccaa catgttcgat cagccggaat
     8761 tggagacgct gctgcgcgcg aacttgaagc gctatccgca cgccacgctg cgggccaact
     8821 cggaggtgac cgacgtcgcc gcgaccgggg cggaccgcgt gcgggtcacg ttcaccgacc
     8881 ggctcagcgg tcaaaagcac tcggtcgacg cggattacgt gctcggctgc gacggcgcga
     8941 acagcgtggt gcgcgccgcg atcggcgcgt cgatgcgcga catgcgattt caccaacgct
     9001 ggctcgtcgt cgatgtggcg agcgccgaag acctgcacca gtgggacggg gtgcatcaag
     9061 tctgcgaccc acaccgcgcc gcaacgtaca tgcggatcgg ggacacccgg taccgctggg
     9121 agttccagtt actgcc
//
LOCUS       DASWBF010000041         4640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_530380,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000041 DASWBF010000000
VERSION     DASWBF010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530380"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..215
                     /locus_tag="VF874_01165"
                     /pseudo
     CDS             33..215
                     /locus_tag="VF874_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            514..801
                     /locus_tag="VF874_01170"
     CDS             514..801
                     /locus_tag="VF874_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9174746.1"
                     /translation="MRRSIGSYRRHGAILIGDQVYKNVGGVPLGNVVISVISGVATFV
                     WLIVDAPYRLLLAIFVALLDLIPFESTVAGVLVAVVALTVSIQESLAPLGF"
     gene            885..1013
                     /locus_tag="VF874_01175"
     CDS             885..1013
                     /locus_tag="VF874_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174747.1"
                     /translation="MAVLSGAGLLGIIGALVAIPVAAALQLLINGLLFSRLDNLSM"
     gene            1030..3120
                     /gene="tkt"
                     /locus_tag="VF874_01180"
     CDS             1030..3120
                     /gene="tkt"
                     /locus_tag="VF874_01180"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9174748.1"
                     /translation="MTTLEDISALTRPNHPADWTDIDSRAVDTIRVLAADAVQKVGNG
                     HPGTAMSLAPLAYTLFQRQMRHDPSDVHWLGRDRFVLSAGHSSLTLYLQLFLGGFGLE
                     LSDLEALRTWGSKTPGHPEFGRTRGVEITTGPLGQGLASAVGMAMASRYERGLLDPDA
                     APGTSPFDHYIYVIASDGDIQEGVTSEASSLAGTQQLGNLIVFYDHNRISIEDDTNIA
                     LSEDTPARYRAYGWHVQEVEGGENVVGIEEAIEAARAVTDRPSFIAIRTIIGYPAPTK
                     MNTGKAHGAALGDEEVAAVKKILGFDPEKTFEMPKGVLEHTRKLIERGKEAHAKWQHD
                     FDAWAACEPERKALLDRLLAQKLPDGWDADLPSWEPDSKPLATRAASGAVLAAVGSKL
                     PELWGGSADLAESNLTTIKGADSFGPPSISTKEWTAHWYGRTLHFGVREHAMGAILSG
                     IVLHGPTRAYGGTFLTFSDYMRPAVRLASLMAIDTIYVWTHDSIGLGEDGPTHQPIEH
                     LAALRAIPNLNVVRPGDANETAYAWRTILARGATSGPVGLILTRQAIPVLEGTSKEGV
                     ARGGYVLGGAPQEDPDVLLIGTGSELQLCVEARKMLAERGIIASVVSMPCVEWFEFQP
                     VEYRDSVLPPAVSARVAVEAAVAQSWYKFVGDRGEIISIEHYGASADYKTIFREFGFT
                     PEAVAAAAERSLHN"
     gene            3137..4267
                     /gene="tal"
                     /locus_tag="VF874_01185"
     CDS             3137..4267
                     /gene="tal"
                     /locus_tag="VF874_01185"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX9174749.1"
                     /translation="MTQEKSQNPNLAALSAAGVSVWLDDLSRDRLRTGNLQELIDTRC
                     VVGVTTNPTIFQKALSEGHAYDEQIAELAERGADVDAAVRTVTTDDVREACDVLRPHW
                     EASDGVDGRVSLEVDPRLAHDTDKTILQAIELCKIVDRPNVLIKIPATRPGLPAITAV
                     LAEGVSVNVTLIFSVERHRAVMDGYLAGLEAAKQAGHDLSKIHSVASFFVSRVDTEID
                     KRLEKVGTDEALALHGQAGVANARLVYAAYQEVFLGGQRFEALKVDGARVQRPLWAST
                     GVKNPDYSDTLYVTELVAPNTVNTMPEKTIDAVADHGVITGDTVTGRAGEAQAVFDKL
                     SAVGVDLRDVFIVLENEGVEKFEASWNELLKETQAQLDAAAK"
     gene            4264..>4640
                     /locus_tag="VF874_01190"
     CDS             4264..>4640
                     /locus_tag="VF874_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016691716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174750.1"
                     /translation="MTTVQSTKSTPAAAAAWVNPLRDKRDKRLPRIAGPCAMVIFGIT
                     GDLARKKLMPAVYDLANRGLLAPTFGLVGFARRDWADEDFATVVYDAVKQHSRTPFRQ
                     EVWDRLAEGKKGLAGAGRAGRLR"
BASE COUNT          774 a         1469 c         1602 g          795 t
ORIGIN      
        1 tctggccggc cagctctagc cgatcggaga acatggtgaa ctcgttggac ccacgtgcct
       61 gggctgcggc ggccatctgc gtgctgattg ccgcggccgc gctgggcggc tgcagcgccg
      121 ggcaacagtc gcaaaccgcc acgatggaac cggccgtcaa cggctccatg gccaccctca
      181 ataacatcgc gctgcgcaac atccggatcc gcgccgagca cagacctcgc agatcagtgc
      241 cgtgcggcgg gtttgcgtct cgcgggccca agcatgccct gtgcgaagga tgccaaagtg
      301 tgacggtgag atgcggagtc ggcctagtgc ggcggcgggg tgagtggcgg ggttgaaccg
      361 gattgcgcac tgaagtggtt tggcgatgca gatatcgggt cagacgccct gcgcgagttg
      421 atgagggccg ggacacggta ttcggcgtgt tgaccgactt tttcatcgtc atcgtgctgg
      481 cggtgtactt cctgggcgct tttcgtcgta tccgtgcggc gctctatcgg ttcctaccgc
      541 cgccacggcg ccattctgat cggcgatcag gtttacaaga acgtgggggg tgtacctctg
      601 ggcaacgtgg tcatttcggt gatcagcggc gtggccacgt tcgtgtggct gatcgtcgac
      661 gctccgtatc ggctgttgct cgccatcttc gtagcactgc tggatctcat tccgttcgaa
      721 tctaccgtcg ctggagtgct ggtcgccgtc gtggcgttga cggtgtcgat ccaggaatcc
      781 cttgccccgc tggggttcta gatcgtgctc cgctggatga aggactacct gctggtgcgg
      841 aggatcatca gacgcggggg gacgttcctg tggtcaccac tttggtggct gtcctgagcg
      901 gcgcaggact gctcgggatc atcggtgcgc tggtcgccat tccagtcgcc gctgcgttgc
      961 agctcctgat caacgggctg ctcttctcac gactcgacaa cctgtcgatg taaaacaggg
     1021 agcgaatacg tgaccacgct tgaggacatt tccgccctta cccgtccaaa tcaccccgcc
     1081 gactggacgg acatcgattc ccgtgcggtc gacaccatcc gggtgctcgc tgccgacgcc
     1141 gtgcaaaagg tcggcaacgg ccatcccggc accgcgatga gcctggcgcc gctggcgtac
     1201 acgctctttc agcggcagat gcgtcacgac cccagcgacg tgcactggct gggccgcgac
     1261 cggttcgtcc tgtcggccgg acacagcagc ctgacgctct acctccagct gttcctcggt
     1321 ggctttgggc tcgaactgtc cgacctcgag gcgctgcgca cgtggggctc gaagacgccg
     1381 ggccacccag agttcggccg caccaggggc gtggagatca cgaccgggcc gctgggccag
     1441 ggcctcgcgt cggcggtcgg gatggcgatg gcgtcgcggt atgagcgcgg cctgttagat
     1501 cccgatgccg cgccgggcac cagcccgttc gaccactaca tctatgtgat cgcctccgac
     1561 ggcgacatcc aggagggcgt gacgtcggag gcgtcgtcgc tggccggcac ccagcaactg
     1621 ggcaacctga tcgtcttcta cgaccacaac cgcatctcga tcgaggacga caccaacatc
     1681 gcgctgtccg aggacacgcc ggcccgctac cgcgcctacg ggtggcatgt gcaggaggtc
     1741 gagggcggcg agaacgtggt cggcatcgag gaggcgatcg aggcggcccg ggccgtcacc
     1801 gaccggccgt cgttcatcgc gatccgcacg atcatcggtt acccggcccc gaccaagatg
     1861 aacaccggca aggcgcacgg cgccgccctg ggcgatgagg aagtcgcggc ggtcaagaag
     1921 atcctgggtt tcgacccgga gaagacgttc gaaatgccca agggtgtgct cgaacacacc
     1981 cgcaagctca tcgagcgtgg caaggaagcc cacgcgaaat ggcagcatga cttcgacgcc
     2041 tgggccgcat gcgagccgga gcgcaaggcg ctgctggacc ggctgctggc gcagaagttg
     2101 cctgacggct gggacgcgga cctaccgtcc tgggagccgg actccaagcc gctggctacc
     2161 cgggccgcct ccggggcagt gctggccgcg gtcgggtcga agctgcccga gctgtggggc
     2221 ggttccgccg acctggccga gagcaacctc accaccatca agggcgcgga ttcctttggg
     2281 ccgccgtcga tttcgaccaa ggagtggacg gcgcactggt acggccggac gctgcatttc
     2341 ggtgtccgtg agcatgcgat gggcgcgatc ctgtcgggca tcgtgctgca cgggccgacg
     2401 cgggcgtacg gcggcacgtt cctgacgttc tccgactata tgcgcccggc ggtgcggctg
     2461 gcctcgctga tggccatcga cacgatctac gtgtggactc acgattcgat cgggctgggc
     2521 gaggacgggc ccacgcatca gccgatcgag catctggccg cgttgcgcgc catcccgaac
     2581 ctcaatgttg ttcgccccgg cgacgcgaac gagaccgcct acgcatggcg gacgatcctt
     2641 gcgcgcgggg cgaccagcgg cccggtgggc ctgattctga cccgccaggc gattccggtg
     2701 ctggagggca ccagcaaaga gggcgtcgcc cggggcggct acgtgctggg cggtgcgccg
     2761 caggaggatc ccgatgtgct gctgatcggc accgggtcgg agctgcaatt gtgcgtcgag
     2821 gcgcggaaga tgttggcgga gcgcggaatt attgccagcg tggtgtcgat gccgtgcgtg
     2881 gaatggttcg agttccagcc cgtcgaatac cgcgacagcg tgctcccacc ggcggtgtcg
     2941 gcgcgggtcg ccgtcgaggc ggccgtcgcg cagagctggt acaagttcgt cggcgatcgc
     3001 ggcgagatca tctcgatcga gcactacggc gcatcagccg actataaaac cattttccgc
     3061 gaattcgggt tcacccccga ggccgtcgcg gccgccgccg aacgatcact gcacaactga
     3121 ggaagcgaat accccgatga cccaagaaaa gtcacagaat cccaacctgg cggcgctgtc
     3181 ggccgccggc gtatctgtct ggctggacga cctgtcgcgc gaccgcttgc gcaccggcaa
     3241 tctgcaggag ctcatcgaca ccaggtgcgt cgtcggcgtc accaccaacc caacgatctt
     3301 ccaaaaggcg ttgtcggaag gccacgccta tgacgagcag atcgccgagc tggccgagcg
     3361 tggcgccgac gtggacgccg ccgtccggac tgtcaccacc gacgacgtcc gcgaagcctg
     3421 tgatgtgttg cggccgcact gggaggcgtc cgacggcgtc gatggccggg tgtcgctcga
     3481 ggttgatccg cggctcgccc acgacaccga caagacgatc ctgcaggcga tcgagctgtg
     3541 caagatcgtc gaccggccca acgtgctgat caagattccc gccacacggc cgggtctgcc
     3601 ggcgatcacc gctgttcttg ccgaaggcgt ttcggtcaac gtgacgctga tcttctcggt
     3661 cgagcgccac cgcgcggtga tggacggcta tctggccggg ctagaagcgg ccaagcaggc
     3721 cggccacgat ctgtcgaaga tccattccgt cgcttcgttt ttcgtctcgc gggtggacac
     3781 cgagatcgac aagcggctgg agaaggtcgg caccgacgag gcgctcgcgc tgcacggcca
     3841 ggccggcgtc gccaacgcgc ggttggtgta tgccgcctac caggaggtgt tcctcggcgg
     3901 tcagcgattt gaggcgctga aggtcgacgg ggcgcgggtg cagcgtccgc tctgggcgtc
     3961 gaccggggtc aagaaccccg actactccga cacgctgtac gtcacggagc ttgtcgcgcc
     4021 gaacaccgta aacacgatgc cggagaagac gatcgacgcg gtcgccgacc acggggtgat
     4081 tacgggtgac accgtcaccg gccgggcggg cgaggcgcaa gcggtgttcg acaagctctc
     4141 ggcggtgggg gtggatctgc gcgatgtgtt catcgtgctc gagaacgagg gcgtcgagaa
     4201 gttcgaggcg tcgtggaacg agctgctgaa ggaaacgcag gctcagctgg acgccgcggc
     4261 gaaatgacga ccgtccagtc gaccaagtcc acaccggccg ctgcggccgc ctgggttaac
     4321 ccgctgcggg acaagcgcga taagcggctg ccgcgtatcg ccggcccctg cgcgatggtg
     4381 atcttcggga tcaccgggga tctggcccgc aagaagctga tgccggcggt ctacgacctg
     4441 gctaatcgcg ggctgctggc gccgacattc ggactcgtcg gtttcgcccg gcgggattgg
     4501 gccgacgagg atttcgccac ggtcgtctac gacgccgtta agcagcattc gcgcacgccg
     4561 ttccggcagg aggtgtggga ccggctggcc gagggcaaga agggactggc tggtgccggg
     4621 cgggctggtc ggttgcgcaa
//
LOCUS       DASWBF010000042         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_399097,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000042 DASWBF010000000
VERSION     DASWBF010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399097"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="VF874_01195"
     CDS             complement(<1..1538)
                     /locus_tag="VF874_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX9174751.1"
                     /translation="MPGRIEIDDVRPVVTCGRYPAKAVVGEIVPVSATVWREGHEAVA
                     ATLVVRNLGRGYPRLAKTADRRTAVPGTATAGLQRVKPILIPMSPGTMPDVFHGEFTP
                     DAVGLWTFRVDGWGDPLGTWRRSVTAKLDAGQGEPELSNDLLVGAKLFERAAMGMPRK
                     LRDPLLEAATALRAPGDPLTRVGAASTDEVTELLAQYPLRELLTRGEQYGVWVDRPLA
                     RFGSWYEMFPRSTGGWGADGKPVHGTFATAAEALPRIAKMGFDVVYLPPIHPIGKVHR
                     KGRNNSVTAAPGDVGSPWAIGSDEGGHDAVHPDLGTIDDFDAFVAAARDQGLEVALDL
                     ALQCAPDHPWAREHRDWFNVLPDGTIAYAENPPKMYQDIYPLNFDIDPDGLYQEVRRV
                     VRHWIDHGVKVFRVDNPHTKRPDFWEWLIEEVKSADADVVFLAEAFTRPARLYGFAKL
                     GFTQSYTYFTWRTAKWELTEFGQQIAEYADYARPNLFVNTPDILHESLQRGGPGMFAI
                     RAVLA"
     gene            1763..4393
                     /gene="glgP"
                     /locus_tag="VF874_01200"
     CDS             1763..4393
                     /gene="glgP"
                     /locus_tag="VF874_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX9174752.1"
                     /translation="MKALRRFTVRAHLPNRLAALEQLSVNLRWSWDKPTQDLFAAIDP
                     ALWERSRQDPVALLGAVTPQRLDELAVDEQFLERLDRLADNLRDYLTRPLWYQEEQAE
                     GAALPTGIAYFSMEFGVSEVLPNYSGGLGILAGDHLKAASDLGLPLIAVGLYYRSGYF
                     RQSLTADGWQQETYPALDPQGLPLRLLTTAAGEPVLVELKLPDDHELRARVWVAQVGR
                     VPLLLLDSDVPENEHDLRGVTDRLYGGDQDHRIKQEILAGVGGVRAIRAFTAAEGISA
                     PEVFHMNEGHAGFLGLERIRELMTDSKLDFDTALTVVRSSTVFTTHTPVPAGIDRFPI
                     DMVEHYFGGDDGKKKESALVPGVPPDRIVALGTEDDTTKFNMAHMGLRLAQRANGVSL
                     LHGRVSRHMFDELWPGFDPDEVPIGSVTNGVHAYTWAAPQWLELGRELIGSDLPSEPA
                     VWERIQQVDSGRLWWIRSQLREQLVNDVRRRLHVSWRQRGAPEAELGWVADAFDPNVL
                     TIGFARRVPTYKRLTLMLRDPERLELLLLDEERPIQMIVAGKSHPADDGGKAMIQQVV
                     RFADRPAVRHRIAFLPDYDMSMARLLYWGCDVWLNNPLRPLEACGTSGMKSALNGGLN
                     LSVRDGWWDEWYDGENGWEIPTADGVSDERRDDLEAAALYHLLESTMAPTFYERDGSE
                     IPRRWVEMVRHTLQVLGPKVLASRMVRDYTEQYYAPAAESLHRTIAPVDGEKFGNARR
                     LANYRRKVSAAWPRIQISEVDSTGLPDTPLLGSKMTLSATVALAGLSPDEVTVQAVLG
                     RVDADDTLVDPARVPMAHTGTTDTGETFATTTPLPVAGSVGYTVRVLPHHPLLAADNE
                     LGLVALARPAAAESPVSVTH"
     gene            4454..>4919
                     /locus_tag="VF874_01205"
     CDS             4454..>4919
                     /locus_tag="VF874_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HEX9174753.1"
                     /translation="MVVSLALAPPVIADPDPVPPPADAAAAAPEAAPDAGPPPDDGRI
                     PSAAPATTKTPDGWTLTISSKDETLLPVAPLTTALSSRDYVVGGTYSAKLNGSGEPRG
                     ILEVGYQIGCGIDMSTSNGVTLAGTAGMTTSLGTTGPFLAPPTGFLPILSFPV"
BASE COUNT          774 a         1646 c         1668 g          831 t
ORIGIN      
        1 gcgaggaccg cgcggatggc gaacatgccg gggccgccgc gctgcaggct ctcgtgcagg
       61 atgtcgggcg tgttgacgaa caggttcggc cgtgcgtagt cggcgtattc ggcgatctgc
      121 tgaccgaact cggtgagttc ccacttcgcg gtgcgccagg tgaaatatgt gtacgactgg
      181 gtgaatccga gcttcgcgaa gccgtacagg cgcgccggac gagtgaacgc ctcggcgagg
      241 aacaccacgt cagcgtcggc gctcttgacc tcttcgatca accactccca gaagtccggg
      301 cgcttggtgt gcgggttgtc gacgcgaaac accttgacgc cgtggtcaat ccagtgccgc
      361 accacccgcc gcacttcctg gtatagcccg tcggggtcga tgtcgaagtt cagcggatag
      421 atgtcctggt acatcttcgg cgggttctcc gcatacgcga tggtcccgtc gggcagcacg
      481 ttgaaccagt cgcggtgttc acgtgcccac ggatggtcgg gcgcgcactg cagcgcgagg
      541 tcgagcgcca cctccaaccc ttggtcgcgg gcagcggcga cgaacgcgtc gaagtcgtcg
      601 atggtgccca gatcggggtg cacggcgtca tgaccaccct cgtcgctgcc gatggcccac
      661 ggcgacccaa cgtcgccggg tgcggcggtg accgagttgt tgcggccctt gcggtgcacc
      721 ttgccgatcg gatggatcgg cggcaggtac accacgtcga agcccatctt cgcgatccgt
      781 ggcaacgcct cggcggcggt ggcgaacgtg ccgtgcaccg gcttgccgtc ggcaccccag
      841 ccgcccgtgg atcgggggaa catctcgtac cacgagccga accgggccag cgggcggtcc
      901 acccacaccc cgtactgctc gccgcgggtc agcagctcgc gcagcggata ctgcgccagc
      961 agctcggtga cctcgtcggt ggacgcggcg ccgacgcggg ttagtgggtc gccgggtgcg
     1021 cgcagcgccg tcgccgcctc cagcagcggg tcgcgcagtt ttcgcggcat gcccatcgcg
     1081 gcccgctcga agagcttggc acccaccaga aggtcgttcg agagctctgg ctcgccctga
     1141 ccggcgtcga gcttcgcggt gacggagcgc cgccaggtgc cgaggggatc gccccagccg
     1201 tcgacccgga aggtccacag gccaacggcg tccggggtga actcgccgtg gaacacgtcc
     1261 ggcatcgtgc ccggcgacat cgggatcagg atcggcttga cgcgttgcag cccggcggtc
     1321 gcagtgcccg gcacggcggt tctccggtcg gcggtcttgg ccaggcgcgg gtagccgcgg
     1381 cccaggttgc ggaccaccaa tgttgcagcc accgcctcgt ggccttcccg ccacacggtg
     1441 gccgacaccg gcacgatctc gccgacgacg gccttggccg gataccttcc gcaggtgacg
     1501 acgggccgca cgtcatcgat ttcgattcga ccgggcaccc gacactcctt cgatgacacg
     1561 gcacgcgcat gccgcggttg tggttcggct gtcgtatcct tcttgtcccg ctgttcccac
     1621 cgtagtgtcg gtcccaaacg ccgggtgcgc gatgtgacca attccgcggc ggccggcgag
     1681 cgcgcttact ccgctttttg gtcttcgcgc aagcggctca tctccgctgc ggtcgttcga
     1741 attctcagta aggtaagcgc gtgtgaaggc actgcgtcgt ttcaccgtcc gtgctcattt
     1801 gcccaaccgc ctcgccgcgc tcgagcagct gtcggtgaac ctgcgatggt cctgggacaa
     1861 gcccacccag gatctgttcg ccgccatcga tcccgcgctg tgggagcgct cccgacagga
     1921 tccagtcgcg ctgttggggg cggtgacgcc gcagcgtctg gacgagctcg ccgtcgatga
     1981 gcagttcctc gaacggctcg accggctggc cgacaatctg cgcgactatc tgacccggcc
     2041 gctgtggtac caggaagagc aggcggaggg tgcggcgctg cccaccggaa tcgcctactt
     2101 ctcgatggag ttcggcgtct cggaggtgct gcccaactat tcaggcggtc tcggcatcct
     2161 ggccggcgat cacctcaagg ccgcctccga cctgggtctg ccgttgatcg cagtgggcct
     2221 ctactaccgg tccgggtact tccgccagtc actgaccgca gacggctggc agcaggaaac
     2281 gtatccggcc ctagatccgc aggggttgcc gttgcggctg ctgaccaccg cagcgggcga
     2341 gccggtgctg gtggagctca agctgccgga cgaccatgag ctgcgcgcca gggtctgggt
     2401 cgcacaggtg ggccgagtcc cgttgctact gctcgattcc gatgtccccg aaaacgagca
     2461 cgacttacgc ggcgtcactg atcggctgta cggcggggac caggaccacc gcatcaagca
     2521 ggagattctg gccggcgtcg gaggagtgcg ggcgatccgg gcgttcaccg ccgccgaagg
     2581 catctccgcg cctgaggtgt tccacatgaa cgagggccac gccggtttcc tcggcttgga
     2641 gcgcatccgc gagttgatga ccgattccaa gctggatttc gacaccgcgc tgaccgtggt
     2701 gcggtccagc accgtgttca ccacgcacac cccggtgccg gccggcatcg accgcttccc
     2761 gatcgacatg gtcgaacatt atttcggcgg cgacgacggc aagaagaaag agtccgcgct
     2821 ggtgcccggc gtgccgccgg accggatcgt cgcgctcggc accgaggacg acacgaccaa
     2881 gttcaacatg gcgcacatgg gccttcggct ggcgcagcgc gccaacggcg tctcgttgct
     2941 gcacggccgc gtcagccggc acatgttcga cgagctgtgg cccggattcg accccgacga
     3001 ggtgcctatc gggtcggtga ccaacggggt gcacgcctac acctgggccg cgccgcagtg
     3061 gctggagctg ggtcgtgagc tgatcggctc ggacttgccg agcgagccgg ccgtgtggga
     3121 gagaatccag caggtggatt ccggtcgcct ctggtggatc cgctctcagc tgcgcgagca
     3181 gcttgtcaac gacgtccgcc gccggctgca cgtgtcctgg cgccagcgcg gtgccccaga
     3241 agccgaatta ggctgggtcg cagatgcttt cgatcccaat gtgctgacca tcgggttcgc
     3301 ccgccgggtg ccgacctaca agagactaac cctgatgctg cgcgaccccg aacgtctgga
     3361 acttctcctg ttggatgagg agcggccgat tcagatgatc gtcgccggga agtcgcaccc
     3421 ggccgacgac ggcggcaagg ccatgattca acaggtcgtg cggttcgccg accggccggc
     3481 ggtgcggcac cggattgcgt ttcttccgga ctacgacatg tccatggcgc ggctgctgta
     3541 ctggggctgc gacgtgtggc tgaacaatcc actgcgtcca ctggaggcgt gtggtacgtc
     3601 cggaatgaag agcgcgctga acggtggact caatttgtcg gtccgcgacg gctggtggga
     3661 cgagtggtac gacggcgaaa acggttggga gatcccgacc gccgatggtg tctccgacga
     3721 acgccgcgac gacctggaag ccgccgcact ttaccacttg ctcgaatcca cgatggcgcc
     3781 gacgttctac gagcgcgacg ggtcggagat tccccgccgc tgggtggaga tggtgcggca
     3841 cacgctgcag gtgctgggac caaaggtgtt ggcgtcgagg atggttcgcg actacaccga
     3901 gcagtactac gcgccggccg cggagtcgct gcaccggacg atcgcgccgg tcgacggcga
     3961 gaaattcggc aacgcgcgcc ggttggccaa ctaccggcgc aaggtttccg cggcctggcc
     4021 gaggatccag atcagcgagg tcgacagcac cggcctgccc gatactccgc tgctcggctc
     4081 gaagatgacg ctgagcgcga ccgtagcgct cgccgggctg tcgccagatg aagtcacggt
     4141 gcaagccgtg ctgggccgcg tcgatgcgga tgacacgctg gtcgatcccg cgcgcgtccc
     4201 gatggcgcac acgggcacca ccgacaccgg cgagacgttc gccaccacca cgccgctccc
     4261 ggtggccggg tcggtcggat acacggttcg ggtgctcccg caccatccgc tgctggccgc
     4321 ggataatgaa ctggggctgg tggcgctggc ccggcctgct gcagccgaga gtccggtatc
     4381 tgtcacgcac tgagaccggc tatgtaaagc ttccggcatg gtggtttgtc gcatcgccgc
     4441 ggtcctagcg tccatggtcg tttcgttggc actggcgccg cctgtcatcg ccgaccccga
     4501 cccggtcccg ccgcccgccg acgccgcggc ggcagcgccg gaagccgctc cggacgccgg
     4561 tccgcctccc gacgacgggc ggattccgtc ggcggcgccg gcgacgacga agacaccgga
     4621 cggttggacg ctcacgatct cctccaagga cgagacactg ctgccggtcg caccgctgac
     4681 caccgctttg tcgtcgcgtg actacgtcgt cggcggcacc tacagcgcca aactcaacgg
     4741 gtcaggcgag ccccgcggaa tcctcgaggt ggggtaccag atcggctgcg gcatcgacat
     4801 gagcacgtcg aacggcgtca cgctggcggg aaccgcaggc atgacgacgt ctctcggaac
     4861 gaccgggccg ttcttggctc cgccgacagg attcctaccg atcctgtcgt tcccggtca
//
LOCUS       DASWBF010000043        12113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1054833,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000043 DASWBF010000000
VERSION     DASWBF010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054833"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VF874_01210"
     CDS             complement(<1..1133)
                     /locus_tag="VF874_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9174754.1"
                     /translation="MWQVRSVTRPRRYRSWLVALAVIPALAMGATGCSGNSAPPAPQV
                     IADKGTPYADLLVPKLQASVTDGAVGVAVDQPVTVNAEDGVLGAVSVARTDGTPVAGK
                     LSPDGLTWSTTEPLGYNKQYTLEAEALGLSGVTHQQMTFKTHSPENLTSAYVLPNDGE
                     VVGVGQPVAIRFDENIPNRLAAQRAIKITTNPPVEGAFYWLSNQELRWRPEAYWKPGT
                     QVDVSVNIYGVDMGDGLFGQEDVKTHFTIGDEVIATADDDTKTITVRRNGEVIKTMPT
                     SMGKNSTPTSNGVYLVGDRFTHIIMDSSTYGVPVNSPNGYRTDVDWATQISYSGIFVH
                     SAPWSVGAQGNTNTSHGCLNVSPSNAIWFYNNTKRGDIVEIVNT"
     gene            1294..1369
                     /locus_tag="VF874_01215"
     tRNA            1294..1369
                     /locus_tag="VF874_01215"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1327..1329,aa:Lys,seq:ctt)
     gene            complement(1407..1901)
                     /locus_tag="VF874_01220"
     CDS             complement(1407..1901)
                     /locus_tag="VF874_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9174755.1"
                     /translation="MTRWYPLKSADADFLASAPHVFRYQKRFAAPPEQVWESLTSDVS
                     IAAWTVAVKEVTWTSPRPFGVGTTREVAAPALSTMRERFFRWDEGSGYSFFVYESTLP
                     PFKRFAEDYVVEPDGADTLFTWTLALEPKDRLALPFKVLAPALKAGFGRIPSDGQRYF
                     AKRM"
     gene            complement(1953..2687)
                     /locus_tag="VF874_01225"
     CDS             complement(1953..2687)
                     /locus_tag="VF874_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX9174756.1"
                     /translation="MTEAVTAEAPQLTKPRILRQDWLNLSFLHWAVAPGSIAHLYPAG
                     TEPDVFEGRSYVGMVPFHMDNTGLPHGPVLLRTFLETNVRLYSVDGTGRRGVVFLSLD
                     ANRLDVVAAARSVFGLPYRWARMSYQSQAGRHTYTSNLRWPGVTAASTVRVQVGDPVK
                     LGPLEHFLTARWGLHLSRAGRTWYLPNDHPAWALRTAELIGFRDDGLLASVGLGDLGR
                     RPPDHVAFSDGVPARFALPVRATTPR"
     gene            complement(3345..3572)
                     /locus_tag="VF874_01230"
     CDS             complement(3345..3572)
                     /locus_tag="VF874_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX9174757.1"
                     /translation="MADRDPEVIKQEIDRTRDQLAATVDTLTERAHPRRLANDLKVAA
                     IAFVKKPPVTFTLIGVGALTVVVVIHRIRNR"
     gene            3673..4140
                     /gene="bcp"
                     /locus_tag="VF874_01235"
     CDS             3673..4140
                     /gene="bcp"
                     /locus_tag="VF874_01235"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX9174758.1"
                     /translation="MTTRLEAGDKAPAFSLEDADGNTVSLADYRGRKLIVYFYPAAST
                     PGCTKQACDFRDNLRVLNKAGLDVVGISPDKPAKLAKFRDAEGLTFPLLSDPDRTVLA
                     NWGAYGEKKMYGKTVQGVIRSTFVVDEDGKIALAQYNIKATGHVDKLRRDLSV"
     gene            complement(4137..5462)
                     /locus_tag="VF874_01240"
     CDS             complement(4137..5462)
                     /locus_tag="VF874_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX9174759.1"
                     /translation="MSDLVERVRDVLPSVRRDLEDLVRIESVWADPSRRSEVQRSAQA
                     VANLLAAAGFDDVEIVAADGAPAVIARRPGPPGTPTVLLYAHHDVQPEGDHGQWMSPP
                     FEPTERDGRLYGRGTADDKAGIATHLAAFRAHQGNPPVGVTVFVEGEEESGSPSLHRL
                     LDAHRDKLAADVIVLADSTNWSTDVPALTVSLRGLVDCVVEVATLDHGLHSGMWGGVV
                     PDALMVLTRLLASLHDDDGNVAVAGLHESDAAELTYPPEQVREETGLLDGVHQIGNGG
                     VPQRLWAKPAITVIGIDTTPVAQSSNTLIPRARAKISMRVAPGGDAKAHLDALTRHLT
                     QHAPWGAHVTVTPGDLGQPYAIDATGPVYDAVRASFRDAWGADPADMGVGGSIPFIAA
                     FADAFPEAKILVTGVEDPATQAHSVNESLDLGVLERAAVAEALLLAKLG"
     gene            complement(5544..5936)
                     /locus_tag="VF874_01245"
     CDS             complement(5544..5936)
                     /locus_tag="VF874_01245"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX9174760.1"
                     /translation="MAIVGLGIDLVFVPDFAEQVDQPGTVFAETFTPGERRDAADKSS
                     SAARHLAARWAAKEAVIKAWSGSRFAQRPVLPEGIHRDIEVVTDMWGRPKVRLSGAIA
                     EHLADVTIHVSLTHEGDTAAAVAVLETS"
     gene            complement(5947..>12113)
                     /locus_tag="VF874_01250"
     CDS             complement(5947..>12113)
                     /locus_tag="VF874_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX9174761.1"
                     /translation="LPAVEDGTARITVAWNPEEVADHTGVTATFGAPLAPTLTVVPDA
                     LVGRCWPAVFAAIGAATTDAGFPVVEGLLSLVHLDHAAYLHTTLPTESAELTVSARTS
                     EATDTEVGRVVPVAVTVTAADGTVLATLTERFAIRGRTGAAELTDPVRAGGAVSDNAT
                     DTPRRRRRDVTITAPTEMRPFAVVSGDHNPIHTDRAAALLAGLKAPIVHGMWLSAAAQ
                     HVATATDGQARPPARLIGWTARFLGMVEPGAEIDFRVDRVGIDQGAEVLEVAARIGSE
                     LVMSATARLAAPKTVYAFPGQGIQHKGMGMEVRARSKAARKVWDTADKFTRATLGFSV
                     LHVVRDNPTSLIAAGVHYQHPEGVLYLTQFTQVAMATVAAAQVAEMREQGAFVEGAIA
                     CGHSVGEYTALACVSGVYELDALLEVVFHRGSKMHDIVPRDELGRSNYRLAAIRPSQI
                     DLADADVPSFIDEIAERTGEFLQIVNYNLRGSQYAIAGTVRGLEALEAEVERRREISG
                     GKRSFILVPGIDVPFHSSVLRVGVPEFRRSLERVMPRDSDPALIVGRYIPNLVPRPFS
                     LDRDFIQEIRDLVPAEPLDEILADYDTWRNERPRELLRKVVIELLAWQFASPVRWIET
                     QDLLFTEEAAGGLGVERFVEIGVKSAPTVTGLASNTLKLTEYAHSTVEVLNSERDVSV
                     LFATDTDPELEHEADEPVESPVAATDFAPAPAAPTAPAGAPEPNVAPTGAPGSNVAPT
                     GAPGPNVAPTGAPRPDDIGFDAADATLALIALSAKMRMDQIEPLDSIESITDGASSRR
                     NQLLVDLGSELNLGAIDGAAEADLSSLKSQVTKLARTYKPFGPVLSDAINDQVRTVLG
                     PSGKRPGYIAERVRKTWELGDGWAKHVTVEVALGTREGHSVRGGDLGGLHDGALTDLP
                     TVDKAIDAAVTSVAARQGVAVALPSASGGGGATVDAAALSEFTEQITGRGGVLANAAR
                     LVLNQLGLDDPVTTPPTATDAELVDLVTAELGSDWPRLVAPVFDRRKAVVFDDRWASA
                     REDLVKIWLMDDDAVNADWPKLAERFEGAGHVVSTQATWWQGKALAAGRNLHASLFAR
                     AAAGAENPGKGRYSDDVAVVTGASKGSIAASVVAQLLDGGATVIATTSRLDDDRLAFY
                     RTLYRDHARFGATLWVVPANMASYSDIDALVSWIGSEQVESLGPQSIHIKDAQTPTLL
                     FPFAAPRVAGDLSEAGARAEMEMKVLLWAVQRLIGGLSGIGAERDIASRLHVVLPGSP
                     NRGMFGGDGAYGEAKSALDAVVSRWKAETSWAQRVSLAHALIGWTRGTGLMGKNDVIV
                     DAVEEAGVTTYSTDEMAAMLLALCDPESRVVASLQPLRADLTGGLGDAQLDMAELAAK
                     AREEMASEPADSEDAAGTIAALPSPPRGYTSAPPPAWDDLDVDPADLVVIVSGAELGP
                     YGSSRTRYEMEVEGELSAAGVLELAWTTGLITWEDDPKPGWYDTETGELVDESELVER
                     YHDAVVQRTGIREFVADGAIDSDHASPLLVSVFLEKDFTFVVSSEADARAFVQFDPEH
                     TVATPMPGTTDWQVTRKAGTEIRVPRKTKLTRTVGGQIPTGFDPTVFGISADMANSID
                     RVALWNIVATVDAFLSAGFTPAELMRWVHPSLVANTQGTGIGGLSSMQTMFHGNLLGR
                     NKPNDILQEVLPNVVAAHVVQSYVGSYGAMIHPVAACATAAVSVEEGVDKIRLGKAEL
                     VVAGGFDDLTMEAIIGFGDMAATADTEMMRAKGISDARFSRANDRRRLGFVEAQGGGT
                     ILLARGDLAVKMGLPVLAVVAYAQSFADGVHTSIPAPGLGALGAGRGGKGSVLARSLA
                     KLGVNADDVAVVSKHDTSTLANDPNETELHERLADSLGRSDGAPLFVVSQKSLTGHAK
                     GGAAVFQIMGLCQILRDGVVPPNRSLDCVDDEMAGSAHFVWPRETLRLGEKFPLKAGL
                     VTSLGFGHVSGLVALVHPQALLATLSDAERQDYEQRAGTRVMAGQQRLASAIAGGRPL
                     YERPADRRFDHDGSEKRQEAEMLLNPASRLGDGDVYVK"
BASE COUNT         1932 a         4114 c         4050 g         2017 t
ORIGIN      
        1 gtgttgacga tttcgacgat gtcgccgcgt ttggtgttgt tgtagaacca gatcgcgttg
       61 ctgggactga cgttgaggca cccgtggctg gtgttggtgt tgccttgcgc gcccaccgac
      121 cacggcgctg agtgcacgaa gatgccgctg taggaaatct gcgtcgccca gtccacgtcc
      181 gtgcgatacc cgttgggtga gttgaccgga acgccgtagg tcgacgagtc catgatgatg
      241 tgggtgaacc ggtccccgac gaggtagacg ccgttgctgg tcggagtgct gttcttgccc
      301 atcgaggtgg gcatcgtctt gattacctcg ccgttgcgtc ggacggtgat cgtcttcgtg
      361 tcgtcgtcgg cggtggcgat cacctcgtcg ccaatcgtga agtgagtctt gacatcttct
      421 tggccgaaca gaccgtcgcc catgtcgacg ccgtagatgt tgaccgacac gtcgacctga
      481 gtgccgggct tccagtaagc ctcagggcgc caacgcaatt cctggttgct caaccagtaa
      541 aacgcgcctt cgaccggtgg gttcgtggtg attttgatcg cgcgctgggc ggcgagccgg
      601 ttcgggatgt tctcgtcgaa gcggatcgcc accggctgtc cgacgccgac tacctcgccg
      661 tcgttcggca ggacgtaggc cgacgtgagg ttttccggcg aatgggtttt gaacgtcatc
      721 tgctggtggg tgacgccgct gaggccaagc gcttcggcct ccagcgtgta ctgcttgttg
      781 tagcccagcg gctccgtagt cgaccaggtc agaccgtcgg ggctgagctt tccggccacc
      841 ggcgtgccgt ccgtcctggc cacgctgaca gcgcccagca cgccgtcctc ggcgttgacg
      901 gtcaccggct gatccaccgc gacaccgact gcgccgtcgg tgaccgacgc ctgcaacttg
      961 ggcaccagaa ggtcggcgta tggcgtgccc ttgtcggcga ttacctgcgg agcaggcggt
     1021 gcggagttgc cgctgcagcc ggtggcgccc atagccagcg ccgggatgac ggcaagagcg
     1081 accagccagg accgataccg acgcggacgg gtcaccgaac ggacctgcca catactttgc
     1141 tccacctcac tgccacggct aactcgttgg cctttttggc aatagtctaa gggcatcacg
     1201 ggcgtgcagc gcgccactgg ctggtaatgc cgttccgttg acaggtgcca gctttggtcg
     1261 gcgcgggcta gcctgttatc gtcgcctgtc gacgcgccgt tagctcagtt ggtagagcag
     1321 ctgactctta atcagcgggt ccggggttcg aaaccctgac ggcgcaccaa gtgtgagttt
     1381 gtggcggaga tcactggtcc ctgtcgtcac atccgcttgg cgaaatagcg ttgaccgtcg
     1441 ctcgggatgc gcccgaaacc ggccttgagc gccggggcaa gcaccttgaa cggcaacgcg
     1501 aggcggtcct tcggctccag cgccaaggtc cacgtgaaca atgtgtccgc accgtcaggt
     1561 tccacgacgt agtcctcggc gaaacgtttg aagggcggaa gggtggactc gtagacgaag
     1621 aacgaatacc cgctgccctc atcccaccgg aaaaaccgtt cccgcatcgt tgacagggcg
     1681 ggcgccgcca cctcgcgggt ggtgcccacc ccgaacgggc gcggcgaggt ccaggtgacc
     1741 tccttgactg cgaccgtcca cgccgcgatc gacacatccg acgtcagcga ttcccaaacc
     1801 tgctcgggcg gtgcggcgaa ccgcttctgg tagcggaaga cgtggggggc cgaggcgagg
     1861 aaatcggcat cggcggactt gagcgggtac cagcgcgtca tagctgacca gcatgccgcc
     1921 caacggacac aacggcgcgg ctattcggaa agtcatcgag gcgtcgtcgc ccgcaccggc
     1981 agggcaaagc gggccggcac gccgtcgctg aaggccacat ggtctggtgg ccgcctgccg
     2041 aggtcgccta ggcccaccga tgccagcaga ccgtcgtccc gaaagccaat cagctcggcg
     2101 gttcgaagcg cccacgccgg gtggtcattg ggcagatacc acgtgcggcc ggcgcgcgac
     2161 agatgcaagc cccagcgggc ggtcaagaag tgctccagcg gacccagttt caccggatcg
     2221 ccaacctgca cccggactgt gctcgcggcg gtgacacccg gccagcgcag gttcgacgtg
     2281 taggtatgac gtcctgcttg gctttggtag ctcatccggg cccagcggta gggcaggccg
     2341 aagacgctcc tggccgcggc gacgacgtcg agccggttgg cgtcaaggct gaggaagacg
     2401 accccgcggc ggccggtccc gtcgacggag tacagccgga cgttggtctc caggaacgtg
     2461 cggagcagca ccggaccatg cggcagtccg gtgttgtcca tatgaaacgg caccatgccc
     2521 acgtaactac ggccctcgaa cacgtcgggc tcggttccgg ccgggtacaa gtgcgcgatg
     2581 ctccccggcg cgaccgccca gtgcaggaac gacaagttga gccagtcctg ccgcagaatc
     2641 cgcggcttgg ttagctgcgg cgcctctgcc gtcaccgcct cggtcatacc ggcaatctag
     2701 ccagaggcgc gcggggtgtt cacgatatgg cgggcggtct gtggcgacga ctggtcgagt
     2761 tcgacgactc cctttgtagc gcaaagactt ccattaacgc caattgaacg caggattcgt
     2821 cgtgaggttg cccgggcggc tgatccagcg tgtctgtttc tccgcctgcg cgcgcggtca
     2881 cgtcctggca ggatcgggcc gtcccgtcga ggtcgacgcc gatttcgtcg ccggacacta
     2941 accggcgccc ccaaccgtga gcaacgtccc cggcaagcgt ctcgcccgcg gaaaccttgc
     3001 attttcggtt attgatgata gtttgcctgg tggttgaaat ttgggcgcgt cgagctgcta
     3061 acatcagtta gctgaagtga cggctgatgc agcagatgac cggggaacat ggcggaatcg
     3121 aggttcagca catgtcagcg agccagctag acggttcgca gcctgcgggt tctcggggtg
     3181 cttctgccat tgctgagcaa tcgcctagcc ggtacacgcc gatgcctgcc acgattcact
     3241 cctgcaaccg gttggggcga gtgggcagcc tgccatttca taccgtgctt ttcggcggtt
     3301 ggtggcggcg ccgcgcgaat cagggcgagt aggcccgcgc cgcgtcagcg gttgcggatc
     3361 ctgtgaatca ccaccaccac cgtcagcgcg ccgacaccga tcaacgtgaa cgtcacgggt
     3421 ggcttcttta cgaatgcgat cgcggcgacc ttcaggtcgt tggcgaggcg gcgcggatgc
     3481 gctcgctcgg tcagggtgtc aacggtggca gccaactggt cccgggtacg gtcgatctcc
     3541 tgcttgatga cttcggggtc acggtccgcc acaatgtcct ccgcgcgtga aggttgttgc
     3601 cttgccgcac taccctagat cagcccgggc aggaaccaac gcaccggtac gcgaccaagg
     3661 ggaaagcttc gaatgaccac gcgactcgaa gcgggcgaca aggcgcccgc gttcagtctt
     3721 gaggatgccg acggaaacac cgtatcgctg gccgactacc gcggccggaa gctgatcgtg
     3781 tacttctacc ccgccgcgtc gacgcccggt tgcacgaaac aggcgtgtga cttccgcgac
     3841 aacttgcgtg ttctgaacaa agcagggctg gacgttgtcg gcatctcccc cgacaaaccc
     3901 gccaagctcg ccaagttccg cgacgcggaa ggcttgacct tcccactgct gtccgatccc
     3961 gatcgcaccg tgttggccaa ctggggcgca tacggcgaga agaagatgta cggcaagacc
     4021 gttcagggcg tgatccggtc gacgttcgtg gtcgacgagg acggcaagat cgcactggcg
     4081 cagtacaaca tcaaggctac cggccacgtc gacaagctgc gccgcgacct gtccgtctaa
     4141 cccagcttcg ccaacagcag cgcctcggcc accgcagcgc gttcgagcac accgagatcc
     4201 aggctctcgt tgacgctatg tgcttgtgtc gcagggtctt cgacgccggt caccaagatc
     4261 ttggcctccg ggaacgcgtc ggcgaacgcg gcgatgaacg gaatcgatcc gcccacgccc
     4321 atgtcggccg ggtcggcgcc ccacgcgtcg cggaacgaag cccgcaccgc gtcatatacc
     4381 gggccggtgg cgtcgatcgc atacggctga ccgaggtcgc ccggcgtgac cgtcacgtgc
     4441 gctccccacg gcgcgtgctg cgttaagtga cgggtcagcg cgtcgaggtg agccttcgcg
     4501 tcgccgcccg gtgcgacgcg catgctgatc ttggcgcgcg cccgcggaat cagtgtgttc
     4561 gacgactgcg ctaccggggt ggtgtcgatg ccgatgacgg tgatcgccgg cttcgcccag
     4621 agcctttgag gcacgccgcc gttgccgatc tggtggacgc cgtcgagcag tccggtttct
     4681 tcgcggacct gctccggcgg ataagtcagc tctgcggcgt cgctttcgtg cagcccggcg
     4741 actgcgacgt tgccgtcgtc gtcgtgcagg ctggccaaca gccgcgtcag aaccatcaac
     4801 gcgtccggca ccacgccgcc ccacatcccc gagtgcagac cgtgatcgag ggttgcgact
     4861 tcgacgacgc agtcgaccag gccacgcaac gagacggtga gcgccggcac gtcggtgctc
     4921 cagttggtcg agtcggcgag gacgatcacg tcggccgcca gcttgtcccg gtgcgcgtcg
     4981 agcagtctgt gcagtgaggg cgacccggac tcctcctcgc cttcgacgaa caccgtgaca
     5041 cctaccggcg ggttgccttg gtgcgcgcgg aacgccgcca aatgtgttgc gattccggcc
     5101 ttgtcgtcgg cagtgccgcg tccgtagagc cgaccgtcgc gctcggtcgg ctcgaacggc
     5161 ggggacatcc actggccgtg gtcgccctcg ggctgcacgt cgtgatgcgc gtagagcaac
     5221 accgtcgggg tgccgggcgg cccggggcga cgcgcgatca ccgcgggcgc cccgtccgcc
     5281 gcgacgatct caacatcgtc gaatccagct gctgccaaca ggtttgcgac cgcctgcgcg
     5341 ctgcgctgca cctcgctgcg acgtgacggg tcggcccaca ccgactcgat gcgcaccagg
     5401 tcttccaggt cgcggcgcac cgacggcagc acgtcacgca cgcgttcgac gagatcactc
     5461 atggtgtcga ggctacttgc gccgaaactg tatttcggca ggccgcttct cgtattttcg
     5521 ctgtgaaaat acagtttcgg cgactaggaa gtctccagga cggcgacggc cgcggcggtg
     5581 tcgccttcgt gcgtgagtga cacgtggatc gtcacgtcgg ccagatgctc ggcgatcgcg
     5641 ccggacagcc gcaccttagg ccgtccccac atgtccgtga ccacttcgat gtcacggtga
     5701 atgccctcag gcagcaccgg acgctgggcg aaccgcgaac ccgaccacgc cttgatcacg
     5761 gcctccttgg ccgcccacct ggcggccaga tgccgggccg ccgacgagct cttgtcggcg
     5821 gcgtcacggc gttcgccggg cgtgaacgtc tccgcgaaca ccgtgccagg ctggtcgacc
     5881 tgctcggcga agtcggggac gaagacaagg tcgatcccca gcccgactat cgccatgacc
     5941 cgaacgctac ttcacgtaga catcgccgtc gccgaggcgc gacgccgggt tcagcagcat
     6001 ctctgcctcc tgacgcttct ccgaaccgtc gtggtcgaac cggcggtcgg cgggccgttc
     6061 gtagagcggc cgcccaccgg cgatcgccga ggccagccgt tgctgcccgg ccatcacccg
     6121 ggtgccggcc cgctgctcgt agtcctgccg ctctgcgtcg gaaagcgtcg ccagcagcgc
     6181 ctgcgggtgc acgagcgcaa ccagacccga cacatggccg aacccaaggc ttgtcaccaa
     6241 cccggccttc agcgggaact tctcacccag ccgcagggtt tcgcgcggcc agacgaagtg
     6301 cgcggagccc gccatctcgt cgtcgacaca atccaggctg cggttcggcg gcaccacgcc
     6361 gtcccgaagg atctggcaca gccccatgat ctggaacacc gcagcgccgc ccttcgcatg
     6421 accggtcagg ctcttctgtg acaccacgaa cagcggagcg ccatcggagc ggcccagcga
     6481 gtccgcgagc cgctcgtgca gctcggtctc gttcgggtcg ttggccagcg tcgacgtgtc
     6541 gtgcttggaa accaccgcga cgtcgtcggc gttcacgccg agcttggcca gtgaccgtgc
     6601 caataccgag cccttgccgc cgcggccggc gcccagcgcg cccaggcccg gcgccgggat
     6661 cgaagtgtgc acgccgtcgg cgaagctctg cgcgtaggcg accacggcca gcaccggcag
     6721 gcccatcttc acggccaggt cgccgcgggc cagcaggatc gtgccgccgc cctgggcctc
     6781 gacgaacccg agccgacgac ggtcgttggc gcgggagaac cgtgcgtcgc tgatgccttt
     6841 ggcccgcatc atctcggtgt cggcggtggc cgccatgtca ccgaacccga tgatcgcctc
     6901 catcgtcagg tcgtcgaagc cgccagccac caccagctcg gccttgccca gccggatctt
     6961 gtcgacgccc tcctccaccg acaccgccgc ggtggcgcat gcggcgacgg ggtggatcat
     7021 cgcgccgtag ctgccgacgt agctctgaac cacatgcgcc gcaacgacat tcggcaggac
     7081 ttcctgcagg atgtcgttcg gcttgttccg gccgagcaga ttgccgtgga acatcgtctg
     7141 catcgacgac aggccgccta tgccggtgcc ctgcgtgttg gccaccagac tcggatgcac
     7201 ccagcgcatc agctcggccg gcgtgaaccc ggcggacagg aacgcgtcga cggtggcgac
     7261 gatgttccac agcgccaccc ggtcgatcga gttggccatg tcggcgctga tgccgaacac
     7321 ggtcgggtcg aacccggtcg ggatctggcc gcccaccgtc cgggtcagct tcgtcttgcg
     7381 cggcactcga atctcggtgc cggccttgcg ggtgacctgc cagtcggtgg tgccgggcat
     7441 cggcgtcgcg acggtgtgct cggggtcgaa ctgcacgaac gcgcgcgcgt cggcctcgct
     7501 ggagacgacg aacgtgaagt ccttctccag gaacacggac accagaagcg gtgacgcgtg
     7561 gtcggagtcg atcgcgccgt cggcgacgaa ctcgcgaatg ccggtgcgct gcacgaccgc
     7621 gtcgtgatac cgctcgacga gctcggattc gtcgacgagc tcaccggttt cggtgtcata
     7681 ccagcccggc ttcgggtcgt cctcccacgt gatgagcccg gtcgtccagg ccagttccag
     7741 cacgccggcg gccgacagct cgccctcgac ctccatctcg tagcgggtgc gcgacgagcc
     7801 gtacggtccg agctcggcgc cgctgacgat caccaccagg tcggccgggt cgacgtcgag
     7861 atcgtcccac gccggcggcg gcgccgacgt gtagccccgg ggtggcgacg gcagcgcggc
     7921 gatagtgcca gcagcgtcct cggaatccgc tggctcgctt gccatttcct cgcgggcctt
     7981 cgcagccaac tcggccatgt cgagctgggc gtcacccaga ccaccggtca ggtcggcgcg
     8041 cagcggttga agtgaagcga cgacccgtga ctcggggtcg cacagcgcca acagcatcgc
     8101 ggccatctcg tcggtggaat acgtggtgac gccggcttct tcgaccgcat cgacgatgac
     8161 gtcgttcttg cccataagcc cggtgccgcg ggtccagccg atcagcgcgt gcgccaacga
     8221 cacccgctgc gcccacgacg tctcggcctt ccaccgcgac accactgcgt cgagcgcgga
     8281 cttggcttcg ccgtaggcgc cgtcgccgcc gaacatgccg cggtttggcg agccgggcag
     8341 caccacgtgc agccgcgatg cgatgtcgcg ctcggcgccg atccccgaca gcccgccgat
     8401 gagccgctgc acggcccaca gcagcacctt catctccatc tcggcacggg caccggcctc
     8461 cgagaggtcg ccggcgaccc gcggtgccgc gaacgggaac agcagtgtcg gggtttgcgc
     8521 gtccttgatg tgaattgact gcggcccaag gctttccacc tgctcgctgc cgatccagga
     8581 gaccagtgcg tcgatgtcgg agtacgacgc catgttggcc ggcaccaccc aaagcgtggc
     8641 gccgaaccgg gcgtggtcgc ggtacagggt gcggtagaac gccagccggt cgtcgtccag
     8701 ccgcgacgtc gtcgcgatca cggtcgcgcc gccgtcgagc agctgcgcca ccacggatgc
     8761 tgcgatcgag cccttcgacg cgccggtcac caccgcgacg tcgtcgctgt agcggccctt
     8821 gccgggattt tccgcgccgg ccgcggcgcg agcgaacagg gaggcgtgca ggttgcgtcc
     8881 cgcggccaac gccttgccct gccaccaggt ggcctgggtg ctgacgacgt gcccggcgcc
     8941 ctcgaaccgt tcggcgagtt tcggccagtc ggcgttgacg gcgtcgtcgt ccatcagcca
     9001 gatcttgacc aggtcctcgc gagcgctggc ccagcggtcg tcgaagacca cggccttgcg
     9061 gcgatcgaac accggcgcga cgagccgcgg ccaatccgag ccgagctcgg cggtgacgag
     9121 gtcgacgagc tccgcgtcgg tggcggtcgg cggtgtggtg accgggtcgt cgagtccgag
     9181 ttggttgagc accaaccgag ccgcgttggc gagcaccccg ccgcggccgg tgatctgctc
     9241 ggtgaactca ctcagcgcgg ctgcgtcgac ggttgcgccg ccaccgccac tggcggacgg
     9301 cagcgcgacg gccacacctt ggcgcgcggc caccgaggtg accgccgcgt cgatcgcctt
     9361 gtcgactgtc gggaggtcag tgagtgcgcc gtcgtgcagg ccgccgaggt ccccgccgcg
     9421 cacgctgtgg ccttcccggg tgccgagtgc gacctcgacc gtgacgtgct tggcccagcc
     9481 gtcgccgagt tcccacgtct tgcggacgcg ctcggcaatg tagccgggcc gcttgccgga
     9541 cgggccaagc acagtgcgca cctggtcgtt gatcgcgtcg gaaagcaccg ggccgaacgg
     9601 cttgtaggtc cgggccagct tggtcacctg cgacttcagg ctcgacaggt ccgcctcggc
     9661 agcgccgtcg atcgcgccga ggttcagctc ggagcccagg tcgacgagca actggttgcg
     9721 tcgcgacgac gcaccgtcgg tgatcgactc gatcgagtcc agcggctcga tctggtccat
     9781 acgcatcttc gccgacagcg cgatcagcgc cagcgtcgcg tcggcggcgt cgaacccgat
     9841 gtcgtcgggc cgcggagcgc cggtgggcgc gacattgggc ccgggagcgc cggtgggcgc
     9901 gacattggac ccgggagcgc cggtgggcgc gacattgggc tcgggtgcgc cggcaggggc
     9961 cgtcggagcg gcgggtgcag gggcaaaatc cgttgcggca accgggcttt cgaccggctc
    10021 atcagcttcg tgctcaagct ccggatcggt gtcggtcgcg aacagcaccg acacgtcgcg
    10081 ctcggaattg agcacttcca ctgtgctgtg ggcgtattcg gtcagcttca acgtgttgga
    10141 cgccagaccc gttaccgtcg gggccgactt caccccgatc tcgacgaacc gttcgacgcc
    10201 gagtccgccc gcagcttcct ccgtgaacag caggtcttgc gtttcgatcc agcgcaccgg
    10261 gctggcgaac tgccaggcga gcagttcgat gacgaccttc cgaagcaact cgcgcgggcg
    10321 ttcgttacgc cacgtgtcgt agtcggcgag aatctcgtcg agcggctcgg ccggcaccag
    10381 gtcgcggatc tcctgaatga agtcgcggtc cagcgagaac ggccgcggca ccaggttcgg
    10441 gatataacgg ccgacgatca gcgcgggatc gctgtcgcgc ggcatgaccc gctccagcga
    10501 gcgccggaac tcgggcacgc cgacccgcag caccgacgag tggaacggca cgtcgatgcc
    10561 gggcaccaga atgaaagacc gcttcccgcc actgatttcg cgacgccgct cgacctcggc
    10621 ctccaacgct tccaggccgc gcaccgtacc ggcgatcgcg tactgcgagc cgcgcagatt
    10681 gtagttcacg atctgcagga attcccctgt gcgctcggcg atttcgtcga tgaagctcgg
    10741 cacgtcggca tcggccaggt cgatctgcga cgggcggatg gcggccagcc ggtagttgga
    10801 ccggcccagc tcgtcgcgcg gcacgatgtc gtgcatcttc gacccacggt ggaacaccac
    10861 ctccagcagc gcgtcgagtt cgtacacgcc gctgacgcac gcgagcgcgg tgtactcgcc
    10921 gaccgagtgc ccgcatgcga tcgcgccttc cacgaacgct ccctgctcac gcatctcggc
    10981 gacctgcgcc gcagcgaccg tggccatcgc gacctgcgtg aactgcgtca gatacagcac
    11041 gccctcggga tgctggtagt gcacgcccgc cgcgatcagg ctggtcgggt tgtcacgaac
    11101 cacatgcagg actgagaagc ccaacgttgc ccgggtgaac ttgtctgcgg tatcccacac
    11161 cttgcgggcc gccttcgatc gggcgcggac ctccatgccc atgcccttgt gctgaatccc
    11221 ctgaccgggg aacgcgtata cggtcttcgg cgcagcgagt cgcgcggtcg ccgacatcac
    11281 caactcgcta ccgatgcgag ctgcgacctc caaaacctct gcgccctggt cgataccgac
    11341 gcggtccacg cggaagtcga tttcggcgcc cggctcgacc atgccgagga accgcgcggt
    11401 ccagccgatc agccgcgccg gcggcctggc ctggccgtcg gtggcggtgg cgacgtgctg
    11461 cgcggccgcc gacagccaca tgccgtgcac aatcggcgct ttcaaaccgg ccagcagcgc
    11521 agcggcgcgg tcggtgtgga tcgggttgtg gtcgccggag acgaccgcga acggccgcat
    11581 ctcggtgggc gcggtgatcg tgacgtcacg gcggcgacga cgcggcgtgt cggtcgcgtt
    11641 gtcggacacc gctcccccgg cgcgcaccgg atcggtgagc tccgccgcac cggtgcgtcc
    11701 acggatcgca aagcgctcgg tcagcgtcgc cagtacggtg ccgtcggccg cggtcaccgt
    11761 gaccgccacc ggcaccacac ggccgacctc cgtgtcggtg gcctcggaag tccttgcgct
    11821 gacggtcaat tcggccgact ccgtcggcag ggtggtgtgc aggtacgcgg catggtcgag
    11881 atggacgagg ctcagcaggc cttcgacgac ggggaaaccg gcgtcggtgg tggcagcacc
    11941 gatggccgcg aagacagcgg gccagcagcg gccgacgagc gcgtcgggca ccacggtcag
    12001 cgtcggcgcg agcggggcgc cgaacgtcgc ggtgacaccg gtgtggtcgg cgacctcttc
    12061 gggattccac gcgacggtga ttcgcgccgt gccgtcctcg acggcgggca ggg
//
LOCUS       DASWBF010000044         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1120580,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000044 DASWBF010000000
VERSION     DASWBF010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120580"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..225)
                     /locus_tag="VF874_01255"
     CDS             complement(31..225)
                     /locus_tag="VF874_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174762.1"
                     /translation="MEEPLLLQHRDERSIVALSLNRPQAFNALSEAMLAALGEAFDAL
                     AAAAHRNAVRRFASSTVNQS"
     gene            complement(236..1885)
                     /locus_tag="VF874_01260"
     CDS             complement(236..1885)
                     /locus_tag="VF874_01260"
                     /inference="COORDINATES: protein motif:HMM:NF006020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9174763.1"
                     /translation="MSNIYDQYLEENRANYAALSPVSFVERSAEVFGDLPAVIHGARR
                     YDWRALRDRSARLAAALRALGVGRGTTVTTMLPNTPEMVEAHYAVPALNAVINTLNTR
                     LDAPMIAWQMNHSEAAVVVTDREFAPVMGPALAMLKEEHGRSPVVIDVCDSEYTGPGE
                     RLGAHEYETLLAAHEPVERLEGPADEWDAIAVGYTSGTTGDPKGVVTHHRGAYLNAVC
                     NAVTWTMPHFPRYLWTLPMFHCNGWCFPWTIAMLGGVHVCLRHVDAPSILAAMREHGA
                     DHFCAAPIVHNLIIAAPAELREGIRQKVRGIVAATAPPAAMIEGMAKIGFDITHVYGL
                     TEVYGPASVAVKRDSWAGESLSEQTRLNGRQGVRYVLQEGMTVLDPETMREVPADGRT
                     MGEIMFRGNIVMKGYLKNPSTTDNAFAGGWFHTGDLAVLEPDRYVKIKDRSKDIIISG
                     GENISSIEVEDALYRHPAVMACAVVAKPHPKWGETPLAYVELTPGATATAQELIAHCR
                     RLLAGYKVPKEVRFEAIPKTSTGKIQKFQLRERAKSASAIE"
     gene            complement(1947..2171)
                     /locus_tag="VF874_01265"
     CDS             complement(1947..2171)
                     /locus_tag="VF874_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX9174764.1"
                     /translation="MGVPSGSTRPRCWPIRPGFQQHGRPTVAVSLLMLSIHPASMTHR
                     RFDEAQQRAAGVLPDMIRMSVGLEHIDNFL"
     gene            complement(2282..2874)
                     /locus_tag="VF874_01270"
                     /pseudo
     CDS             complement(2282..2874)
                     /locus_tag="VF874_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787931.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
     gene            2914..3117
                     /locus_tag="VF874_01275"
                     /pseudo
     CDS             2914..3117
                     /locus_tag="VF874_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016932625.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          486 a         1103 c          999 g          529 t
ORIGIN      
        1 cctatgggac agcccccgac aacgcgacgc ctatgactgg ttcaccgtcg agctggcgaa
       61 cctgcgcacc gcgttccggt gggccgccgc agcgagcgcg tcgaaggcct cgcccagcgc
      121 cgcgagcatc gcctccgaca gtgcgttgaa ggcctgcggc cggttcagcg acagcgcgac
      181 aatgctgcgt tcgtcgcggt gttgcagcag cagcggttct tccatcgcga tgacctcact
      241 cgatcgccga ggcgctcttc gcgcgctcac gcagctggaa cttctggatc ttgccggtcg
      301 aggtcttcgg gatcgcctcg aagcggacct ccttcggcac cttgtagccc gcgagcaggc
      361 gccggcagtg ggcgatcagc tcctgtgcgg tcgccgtcgc gcccggcgtg agctcgacgt
      421 aagccagcgg cgtctcgccc cacttcgggt gcggcttcgc gaccaccgcg caggccatca
      481 ccgccgggtg ccggtacagc gcgtcctcga cctcgatcga gctgatgttc tcgccgccgg
      541 agatgatgat gtccttgctg cggtccttga tcttcacgta gcggtccggc tccagcaccg
      601 ccaggtcgcc ggtgtggaac cagccgcccg cgaaggcgtt gtcggtggtc gaggggttct
      661 tcaggtagcc cttcatcacg atgttgccgc ggaacatgat ctcacccatc gtccggccgt
      721 cggcgggcac ctcgcgcatc gtctcagggt ccagcaccgt catgccttcc tgcagcacgt
      781 agcgcacgcc ctggcggccg ttcaggcgcg tctgctccga caggctctcg ccggcccagc
      841 tgtcgcgctt gaccgccacc gaggccggtc cgtagacctc ggtcagcccg tagacgtggg
      901 tgatgtcaaa gccgatcttc gccatcccct cgatcatcgc ggccgggggc gcggtggcgg
      961 cgactatgcc gcgcaccttc tgccggatgc cctcgcgcag ctcggccggc gcggcgatga
     1021 tcaggttgtg cacgatgggc gcggcgcaga agtggtccgc gccgtgctcg cgcatcgcgg
     1081 cgaggatgga cggcgcgtcg acgtggcgca ggcagacgtg cacgccgccg agcatcgcga
     1141 tcgtccacgg gaagcaccaa ccgttgcagt ggaacatcgg cagtgtccac aggtagcgcg
     1201 ggaaatgcgg catcgtccac gtcaccgcgt tgcacaccgc gttgagatag gcgccgcggt
     1261 ggtgcgtcac gacgcctttc gggtcaccgg tggtgcccga ggtgtagccg acggcgatcg
     1321 catcccactc gtcggccggg ccctcgagcc gctcgaccgg ctcatgcgcc gccagcagcg
     1381 tctcgtactc gtgcgcgccg agacgctcac ccgggccggt gtactcgctg tcacagacat
     1441 cgatgaccac cgggctgcgc ccgtgttcct ccttcagcat cgccagcgcg ggccccatca
     1501 ccggcgcaaa ctcgcggtcg gtgaccacga ccgccgcttc gctgtggttc atctgccagg
     1561 cgatcatcgg cgcgtccagg cgcgtgttca gcgtgttgat caccgcattc agcgcaggga
     1621 ccgcgtaatg cgcctcgacc atctccggtg tgttgggcag catcgtggtc accgtcgtgc
     1681 cgcggcccac gcccagcgcg cgcagcgccg cggccaggcg cgccgagcgg tcacgcagcg
     1741 cgcgccagtc gtagcgccgt gcaccgtgga tcaccgccgg caggtcgcca aacacctcgg
     1801 cgctgcgctc gacgaaactc accggagaga gcgctgcgta attcgcgcgg ttctcctcca
     1861 ggtactggtc gtaaatgttg ctcacatcag tctcctgcct ccgccaaggc ctgatcgctc
     1921 gctgtggcga gcgtctcatc gatatctcac aggaaattgt cgatgtgctc tagcccgacc
     1981 gacatccgga tcatgtcggg cagcacgccg gcggcgcgct gctgcgcctc gtcgaaccgg
     2041 cggtgcgtca tcgacgccgg atggatgctc agcatgagaa ggctgaccgc cacggtgggc
     2101 ctgccgtgtt gctggaaccc cggccggatc ggccagcatc gcggccgcgt cgagcccgag
     2161 gggactccca cagcgcggcg tccaacgcgt tggtgctgga ccaacctatc gcgatttggc
     2221 gtgcaccaac cgtgcatcaa cacgccatca ctcccacgcc gggcccctag gccgacctac
     2281 atcagcgtac cgttcccact gccgcttcca gttcggccag gcactgctcg gtgagccctg
     2341 tcaccgcctc gatgtcgggc atcacgtcgc ggcccgccag ataaccgaaa ctaatgcgat
     2401 cggcgtacga gcacaccgtc acgttgagcg ccgtgccgtc ggagaccgtc gacaccggat
     2461 agatctcctc gacatgcgct ccgttccaat acatttcggt ccgtggtccc ggaacattcg
     2521 agatcgacag gttgtatccg gtgcgcacct tcggcacaaa ggcaccatcg gtaacaacag
     2581 cgttggggcg atgctcggcg cgagcagcaa caaggatgcc cccgacccac ggcgggccac
     2641 ctgatgcttt ccttcggcca tcgagcggtg gatcagatcc agccgttcgg cggggtcttc
     2701 caggacggtg cccagcgggc acagccaggc accgaacatg ttgccgtgcc cgtcatccgg
     2761 cccgtgctcc cggccacgca ccgtgatcgg gcaggtggcc accagcgatt gttcgggcag
     2821 ctcgccatga tcgagaagcc agccgcgcaa caccccgccg atcaccgcga ccaaacatgc
     2881 ggaggtccat cagaacttat ctcgcagacg caggcatgga ctggagtgca cgagctgtgg
     2941 ctccgcaagc agcgattcga tgcccccggg gttgcaagct ggcctacgac gccgccttcg
     3001 acgcgatgct ggccaccctg gaccgccggg atcggctcga tcaggcgatc accgcgatgg
     3061 ccgccgattc gccgttcacc ccggtggtgt gtcggttggg ctgcgtgcgc gggattt
//
LOCUS       DASWBF010000045         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1188515,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000045 DASWBF010000000
VERSION     DASWBF010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188515"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..1549
                     /locus_tag="VF874_01280"
     CDS             416..1549
                     /locus_tag="VF874_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX9174765.1"
                     /translation="MGRDPTAPAGEKPAHMVTLADSTCSISRTHLEFWIGNSGLWIRD
                     RHSTNGSEVEVYGRRTRIKPGMAVSVPPTSTIHVGGQRIRVRAVNSRAVIDAVTVEWG
                     AATRVGARRAHNQDTFGARPPVFVVADGIGGHCAGDLASREAVQSLLAVADRAEVTDE
                     MLDNALADARARINRIPVSDGKRPPGTTISGVIVTYCNGVPCWMVVNLGDSRTYRLDA
                     KGLRQLSVDHSVGQKLIEAGVAQAGPQQLPVGNILTRAVVGGREHSPDVWRLPMHAGD
                     RILVCSDGLSSAVDDSSIMRVLQTVRDPQAAVDALIDTAIRAGGGDDATAVVVDAVAI
                     RPRHYSDMSTRAARRGALARDTSTTGRLPTDRTIFTTRARWRA"
     gene            1693..3585
                     /locus_tag="VF874_01285"
     CDS             1693..3585
                     /locus_tag="VF874_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9174766.1"
                     /translation="MTVTQEVTEQQARQVAEDAREARWHQPSFGKELFLGRLRLDLVH
                     PHPSGSAESTDRGEAFLATLREFCESQIDAAVIERESQIPDAVIRGLKERGAFGMKIG
                     PEYGGVGLSQVYYNKALALVGSVHPSLGALLSAHQSIGVPQPISMFGTEEQKRTWLPR
                     CTREISAFLLTEPDVGSDPARLHTTAIPDGDDYVLNGIKLWTTNGVIADLLVVMAKVP
                     KSEGHRGGITAFVMEAGAPGIVVTNRNAFMGLRGIENGLTRLTDVRIPAANRIGREGD
                     GLKIALSTLNVGRLSLPAVCSGAAKWCLKIAREWSRERVQWGRPIGEHEAVAGKVAFI
                     AATAYGLEAMLDLASELADAKSHDIRIEAALAKLYGSEMAWLIADELVQIRGGRGYET
                     AESLAARGERGVPAEQILRDLRINRVFEGSSEIMHLLLAREAVDAHLSVAGDIIDPEA
                     DLRQKAQAAAKAGKFYARWLPTLVTGKGQLPSSFSEFGALAVHLRYVERASRRLARST
                     FYAMSRWQGRLEHKQRFLGRIVDIGAELFAMTAACVRAQRDRADGSTVELADAFCRQA
                     RIRADQRFDELWNNCDDSDQALARRVLDGRYTWLEEGIIDPSIEGPWIAHDGGDVTHD
                     VHRVIT"
BASE COUNT          630 a         1154 c         1285 g          622 t
ORIGIN      
        1 cgcggtttgg cgacacctca gtgttggacc tggaagtcgt tggggcctgg cgctgttcct
       61 ctctgcgact ggcgatcccg atgtcgagtt tcgtgagtaa tgtcgggggg tacgccccat
      121 ccgtcgattt gacggctccg gccacgatct agaggatcca ctgatgcgcg acggcttgac
      181 cacccggtcc ccggttgccg accgtgcgga accttcgcgt gcgtcgaccc gaggcgggcc
      241 cgggccggcg gggggacggc gcccatcctc cgtcgcccca ccgaggacgt cccggacaga
      301 cgcgcgatcg gctggcggtg ttgcatccac cacggtgctg ccgcgcccgc gaccactgcg
      361 gtgtgggctg gaattcgaag acggatcttt tttcgtcgtt cgcggccacg ggctgatcgg
      421 acgcgacccg acggctcctg ccggcgaaaa gccggcgcat atggtgacgc tggccgattc
      481 cacttgctcg atatcgcgga cgcatcttga gttctggatt ggcaacagcg gactgtggat
      541 ccgagaccgc cactccacca acggatctga ggtcgaggtc tacggacggc gaacccggat
      601 aaagccggga atggccgtgt cggtgccacc aacgtcgacg attcacgttg gcgggcagcg
      661 catccgggtg cgggcggtca acagtcgcgc cgtcatcgat gcggtgaccg tcgaatgggg
      721 tgcggccact cgcgtcggcg cgaggcgcgc gcacaaccag gatactttcg gcgcgcgtcc
      781 gccggtattc gttgtggccg acgggatcgg cggccactgc gctggtgacc tggcgagtcg
      841 cgaagcggtg cagtcacttt tagccgtcgc ggaccgcgcc gaggtaaccg acgagatgct
      901 cgacaacgct ctggccgacg cgcgggcgcg catcaaccgg atcccggtca gcgacgggaa
      961 gcgaccaccg ggtaccacga tcagtggtgt gatcgtcacg tactgcaacg gcgtaccgtg
     1021 ctggatggtg gtcaacctcg gcgactctcg cacctaccgg ttggatgcga aaggactgcg
     1081 ccaactgagc gtcgaccatt ccgtgggtca aaagctgatc gaggcgggag tggctcaagc
     1141 cgggccacaa cagttgccgg ttggaaacat cttgacccgg gctgtggttg gcggccgcga
     1201 gcattcgcct gatgtgtggc gactaccgat gcacgccggc gaccggatcc tggtgtgctc
     1261 ggatggcctt tcatcggctg tggacgactc gagcatcatg cgggtgctgc agacggtgcg
     1321 cgatccgcaa gccgccgtgg acgcgttgat cgacaccgcg atccgcgcgg gtggcggcga
     1381 cgatgcgacc gcggtggtgg tcgacgccgt cgcgatcagg ccacgccact attcagacat
     1441 gtcgacgcga gcggcgcggc gcggcgcgct tgcgcgtgac acctcgacga cgggccgact
     1501 gccgaccgat cggaccatct tcaccacccg ggcccgctgg cgcgcgtgat tgtgccgtca
     1561 cggcacgacg gcccaccgga cctgtcggcg agtttgggca caacacgaca tcgctgttcc
     1621 ctggttgcgg ttcctcgctg cgcgctgtcg ggttactgtt cagtaatccc ggcgtctatg
     1681 gaaggttcta ccatgaccgt tacccaagaa gtcaccgagc agcaggccag gcaggtcgcc
     1741 gaagacgcac gagaggcgcg ctggcaccag cccagtttcg gcaaggagtt gttccttggc
     1801 cgattgcggt tggacctggt ccatccgcac cccagcggct cggcggagag taccgatcgt
     1861 ggcgaagcgt tcctggccac gctgcgcgag ttctgcgagt cgcagatcga tgccgctgtg
     1921 atcgagcgtg agtcgcagat ccccgacgcc gtaatccggg gtttgaagga gcgcggtgcc
     1981 ttcggcatga agatcggacc cgagtacggc ggcgtcggcc tgtcacaggt gtactacaac
     2041 aaggcgctcg cgctggtcgg ctcggtacat ccctcgctgg gtgcgttgct ctccgcccac
     2101 cagtcgatcg gggttcccca accgatttcg atgttcggca ccgaggagca gaagcgcacg
     2161 tggctgcccc ggtgcacgcg agagatcagc gcgttcctgc tgacggagcc ggacgtcggc
     2221 agcgacccgg cccggctgca caccaccgcc ataccggacg gcgatgacta cgtgctgaac
     2281 gggatcaagc tgtggaccac caatggcgtg atcgctgacc tgctggtggt catggccaaa
     2341 gtgcccaaga gcgaggggca ccggggtggg atcaccgcgt tcgtgatgga ggcgggtgca
     2401 ccgggcattg tggtaacgaa tcgcaacgcg ttcatgggat tgcgcggcat cgaaaacgga
     2461 ctcactcgac taaccgacgt gcggataccc gcggcgaacc ggattggacg ggaaggcgac
     2521 gggctcaaga tcgcgctgtc gacgctgaat gtaggtcggc tgtcgctgcc cgcggtgtgc
     2581 agtggcgcgg cgaaatggtg tctgaagatc gccagggagt ggtcgcggga acgggtgcag
     2641 tggggccgcc cgattggtga gcacgaagcc gtggcgggca aggtggcgtt catcgcagcc
     2701 accgcctacg gcctggaggc gatgctcgac ttggccagtg agctggccga cgcgaagagc
     2761 cacgacattc ggatcgaggc ggcgctggcc aagctgtacg gctcggagat ggcctggctg
     2821 atcgccgacg agttggtgca gattcgcggc ggacggggct atgagaccgc cgagtcgcta
     2881 gccgcccgcg gcgagcgcgg ggtaccggcc gagcagatct tgcgcgactt gcgaatcaac
     2941 cgggtcttcg agggctcctc cgagatcatg catctgctgc ttgcccgcga ggcggtcgat
     3001 gcccacctgt cggtcgccgg cgacatcatc gacccggagg ctgacctgcg gcagaaggcg
     3061 caagcggccg cgaaggcagg caagttctac gcccgctggc tgcccacctt ggtgaccggc
     3121 aagggccagc tgccctcgtc gttcagcgaa ttcggagctc tggccgtgca tctgcgctat
     3181 gtcgaacggg ccagccgacg actggcccgc agcaccttct acgcgatgtc gcggtggcag
     3241 ggcaggctcg agcacaagca gcggttcctg ggccggattg tggatattgg ggcggagctg
     3301 ttcgcgatga ccgcggcctg cgtccgggcc cagcgcgacc gggcggacgg tagcaccgtc
     3361 gaactcgccg acgctttctg ccgccaggcc cggattcggg ccgaccagcg gttcgacgag
     3421 ctctggaaca actgcgacga ctccgaccaa gcgttggctc gacgtgtact tgacggtcgc
     3481 tacacgtggc tcgaggaagg gatcatcgac ccgtcgatcg aggggccgtg gatcgctcac
     3541 gacggcggcg acgtgacgca cgacgtgcac cgcgtcatca cgtaacaaac ccccaacgtc
     3601 tgactattaa gaagtggggg gtcgccccag aagtagatgg agcattcgct cacacaccga
     3661 cggcggcaag cgagcagtac ccccattcga t
//
LOCUS       DASWBF010000046         5358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_265533,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000046 DASWBF010000000
VERSION     DASWBF010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265533"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VF874_01290"
     CDS             <1..642
                     /locus_tag="VF874_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX9174767.1"
                     /translation="GARALEWMVSHPDNVRSGLLLAVGARATADQVGTQCAQVAAIKA
                     DPNWQGGDYYGTGREPVDGMDVARRFAHLTYRGEVELDRRFANQPQASENPLAGGRYA
                     VQSYLEHQGAKLVDRFDPGSYVVLTDALSSYDVGRGRGGVPSALRHCTVPAVVGGITS
                     DRLYPLRLQEELAELLPGCTGLNVVDSVYGHDGFLIESEAVGDLIRQMLKLAQ"
     gene            648..1400
                     /locus_tag="VF874_01295"
     CDS             648..1400
                     /locus_tag="VF874_01295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9174768.1"
                     /translation="MSPSGEEQTAELARSLSFGAEAAAYERGRPSYPPEAIDWLLPGG
                     ARDVLDLGAGTGKLTTRLVERGLSVMAVDPIPEMLELLRDSLPDTPALLGTAEEIPLP
                     DDSVDAVLVAQAWHWFDPTRAVPEVARVLRPGGRLGLVWNTRDERLGWVRDLGRIIGH
                     EDDPFNNRVTLPAPFADPQRHQLEWTNYLTPQALIDLVASRSYCITSPAEVRTQTLDQ
                     VRELLATHPALANSTGLALPYVTVCIRATLSA"
     gene            1446..1835
                     /locus_tag="VF874_01300"
     CDS             1446..1835
                     /locus_tag="VF874_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174769.1"
                     /translation="MPEATDRVTRIATDLYESATAEGARQSRSAKQQLDHWARVGRAV
                     SSQHTASRRRVEAALAGRLSMGDLTVEEGVVFNAEISAAIEEKLAHTHYGDELAARGI
                     TTVALNESGDIVEYQPDGTTTVLAPKR"
     gene            1839..2417
                     /locus_tag="VF874_01305"
     CDS             1839..2417
                     /locus_tag="VF874_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9174770.1"
                     /translation="MIRLDLVVGPNGAGKSTFVALTLAPLLPESVFVNADEIAQRRWP
                     DDPAARSYDAAQIAAQTRSTLIQQGRPFIAETVFSHPSKLDLIDAAHAAGYTVVLHVL
                     LIPEDLAVERVKHRVRAGGHDVPERKIRERHRRLWHLVADAITKSDIATVYDNSRLDG
                     PRIVAQISGGELVGAFTWPAWTPAVLRSRWPN"
     gene            complement(2420..2713)
                     /locus_tag="VF874_01310"
     CDS             complement(2420..2713)
                     /locus_tag="VF874_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HEX9174771.1"
                     /translation="MTVRDAARRLIRAQWPILLVFLTFVTAFGLVVGDFWRRGSLLIG
                     IGVGLAAVLRLVLSDKRAGLLVVRRRMTDFTTMAVVCATVIYVAWTIDPLGTR"
     gene            complement(2743..3588)
                     /locus_tag="VF874_01315"
     CDS             complement(2743..3588)
                     /locus_tag="VF874_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEX9174772.1"
                     /translation="MTATMLDGKATRDEIFADLTKRVQALAAAGRTPGLGTILVGDDP
                     GSQAYVRGKHADCAKVGITSIRRDLPADISQAKLDETIDELNENPECTGYIVQLPLPG
                     QLDENAALERIDPDKDADGLHPMNLGRLVLGKAAPLPCTPRGIVHLLRRYEVEIAGAH
                     VVVIGRGVTVGRPLGLLLTRRSENATVTLCHTATRDLPALTKQADIVVAAVGVPHMVT
                     ADMVKPGAAVVDVGVSRVDGKLVGDVHPDVWEVAGHVSPNPGGVGPLTRAFLLTNVVE
                     RAEKR"
     gene            3652..4566
                     /locus_tag="VF874_01320"
     CDS             3652..4566
                     /locus_tag="VF874_01320"
                     /inference="COORDINATES: protein motif:HMM:NF017468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9174773.1"
                     /translation="MNQALTHGLPAVVVAAALYGCAPVLQAASVRRAAPAVGLGLRLL
                     PRLAVQPLWLLGTACGIAAFAAEAYAFSVAPAALVAPLVTLDMVFLVLLARHGLSERL
                     GIAGALGILAMVLGTALIAFAFSGDAELGAPASHAQLLAFLVGGTAAAGAAAVVGDRG
                     SRTGRSWLAAIGFGVASGVASGIATLTTRQIGLTFDVHDPWPVLATPTPYALLVASIL
                     ALALLQRGLQSGPSVLTFPVMSFMSAFVPVMIGITVLDDQVPTSGPGVGFVFALLAVA
                     LGVALLARDRAAAEHTMERTDLRVGETA"
     gene            complement(4543..5223)
                     /locus_tag="VF874_01325"
     CDS             complement(4543..5223)
                     /locus_tag="VF874_01325"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX9174774.1"
                     /translation="MNGYAETREHLSRLGIATIGGSDDGLVRTIELDGLTVGFAAFTQ
                     WRNASKAAFAGRVTDLEDLLDDDAAPLHHSRADLLCVVAHWDYEFRHSPRPATRALAH
                     DLAESGAGLIVGHHAHVLQPVERIRDALVAFGVGDFLGTALPRVRWPLRLGGILAVDI
                     SADAATKGEIAAYAVVPFARERAGRHERLVSLDAVAGRVGDLMRERFGQVFPSANGQL
                     ALRRFPHA"
BASE COUNT          830 a         1832 c         1827 g          869 t
ORIGIN      
        1 ggcgcgcggg cgctggagtg gatggtcagc catcccgaca acgtgcggtc ggggctgttg
       61 ctggccgtcg gcgcgcgggc gaccgccgac caggtcggca cgcagtgcgc gcaggtcgcc
      121 gcgatcaagg ccgacccgaa ctggcagggc ggcgactact acggcaccgg gcgcgaaccc
      181 gtcgacggca tggacgtcgc ccggcggttc gcgcacttga cctaccgcgg cgaggtcgaa
      241 ctcgaccggc ggttcgccaa tcagccgcag gcctccgaga acccgctggc cggcggccgg
      301 tatgcggtgc agagctacct cgagcatcag ggcgccaagc tcgtcgaccg gttcgatccg
      361 ggcagctacg tggtgctcac cgatgcgctg tccagctacg acgtcggccg cggccggggc
      421 ggggtgccct cggcactgcg ccactgcacg gtgccggcgg tggtcggcgg catcacgtcg
      481 gaccggctgt atccgctgcg gctgcaggag gagctggccg aactgctgcc cggctgtacc
      541 ggcctgaacg tcgtcgactc ggtctacggc cacgacgggt tcctgatcga gtccgaggcc
      601 gtcggcgact tgatccggca gatgctgaag ctcgcgcagt gacggcgatg agcccctcgg
      661 gcgaagagca gacagccgag ctggctcggt ccctgtcgtt cggggcagag gccgctgcgt
      721 acgagcgggg ccgcccgtcg tatccgccgg aggcgatcga ttggctgctg cccggcggcg
      781 cccgcgacgt gctggacctg ggtgcgggca ccggcaagct caccactcgg ctggtcgagc
      841 gcgggctttc ggtaatggcc gttgatccga tcccggaaat gcttgagctg cttcgtgatt
      901 cgctgcccga cacgcccgcg ttgctcggta cggccgagga gatcccgctg cccgacgaca
      961 gtgtcgacgc ggtactcgtc gcgcaggcct ggcactggtt cgacccgacg cgggccgtcc
     1021 ccgaggtggc gcgggtgctg cggccgggcg gccggctggg actggtgtgg aacacccgcg
     1081 acgagcgcct cgggtgggtg cgcgaccttg gccggatcat cggccacgag gatgacccgt
     1141 tcaacaaccg ggtcacattg cctgcgccgt tcgccgatcc gcaacggcat cagttggaat
     1201 ggacgaacta ccttacgccg caagcactta tcgatctcgt ggcgtcgcgc agctactgca
     1261 tcacgtcgcc ggcggaggtg cgtacccaga cgctggacca ggtgcgcgag ctgctggcga
     1321 cccacccggc gctcgccaac tcgactggcc tggcgttgcc gtacgtcacg gtgtgcatcc
     1381 gggcgacgct ttccgcgtag cagttcgcta caatatgtag cagattgctt caagaggagt
     1441 agacaatgcc ggaagccacc gatcgcgtca cacgcatcgc caccgatttg tacgagagcg
     1501 cgacggcaga gggggcgcgc cagagccgct cggcgaagca gcaactcgac cactgggcac
     1561 gcgtggggcg cgcagtttcg agccagcaca cggcgtctcg gcgtcgagtc gaagccgcgc
     1621 tcgccgggcg tctttccatg ggagatctga ctgtcgaaga gggagtcgta ttcaacgccg
     1681 agatctccgc cgccatcgag gaaaagctgg cacacaccca ctacggtgac gaacttgccg
     1741 cgcgcggcat cacgaccgtg gcgctgaacg agtcgggcga catcgttgag taccagccgg
     1801 acggcacgac aaccgttctg gcgccgaagc gctgagggat gattcggctc gaccttgtgg
     1861 tcggaccaaa cggggccggc aagtcgacgt tcgtcgcact cacgctagcg ccgttgctac
     1921 cggagagcgt attcgtcaac gccgacgaga tcgcccaacg gcgctggcct gacgatcccg
     1981 cagcacggtc gtatgacgcg gctcagatcg ccgctcaaac caggtccaca ctcatccagc
     2041 aaggcaggcc gttcatcgcg gagacagtct tttcgcaccc gtcgaagctc gatctcatcg
     2101 acgcagcaca cgcggccggg tataccgtcg tcctccacgt gttgctcatc ccggaggacc
     2161 ttgccgtcga gcgcgtcaag caccgggttc gggccggcgg tcatgacgtt ccggagcgca
     2221 aaattcgcga gcggcatcgc cgcctatggc acctcgtggc cgacgccatc accaagtccg
     2281 atatcgcgac ggtgtacgac aacagccggc ttgacggacc acgcattgtc gctcagatca
     2341 gcggaggtga actcgtcggc gcgttcactt ggccagcctg gacaccggca gtgcttcggt
     2401 cgcgctggcc gaactagcac tagcgcgtgc cgagtgggtc gatggtccac gccacgtaga
     2461 tcacggtcgc acacaccacc gccatcgtcg tgaagtccgt catccggcgg cggacgacga
     2521 gtagcccagc gcgcttgtcg gacagcacga gccgcagcac cgcggccaat ccgacaccga
     2581 tgccgatcag cagcgacccg cggcgccaga agtcgccgac cacgaggccg aacgcggtga
     2641 cgaacgtcag gaacaccagc aggatcggcc actgggctct gatcagccgg cgggcggcat
     2701 cgcggaccgt catacgctgc tgtctgctct tcgcgcgagc gctcatcgct tttccgcgcg
     2761 ctcaacgaca ttcgtcaaca ggaacgcccg cgtcaacggg ccgacgccgc cggggttcgg
     2821 cgacacgtgc cccgcgacct cccatacgtc gggatggacg tcgccgacga gcttgccgtc
     2881 caccctgctc accccgacgt cgacgacagc ggcgcccggc ttgaccatgt cggccgtcac
     2941 catgtgtggc acgccgacgg ccgcgaccac gatgtcggcc tgcttggtca gcgcgggcag
     3001 gtcgcgagtt gcggtgtggc acaacgtgac tgtggcgttt tccgaacggc gagtcagcag
     3061 cagcccgagg ggccggccca cggtgacgcc gcgaccgatc acgacgacat gggcgccggc
     3121 gatctcgacc tcgtagcggc gcagcagatg cacgatgccg cgcggcgtgc acggcagcgg
     3181 cgccgccttg cccagcacca gccgcccgag gttcatcggg tgcaggccgt cggcgtcctt
     3241 gtccgggtcg atgcgctcca gcgcggcgtt ctcgtccaac tgaccaggca gcggcaactg
     3301 gacgatgtag ccggtgcact cggggttctc gttgagttcg tcgatcgtct cgtcgagctt
     3361 ggcctggctg atgtcggcgg gcaggtcgcg ccggatcgac gtgatgccga ccttggcaca
     3421 gtcggcgtgc ttgccgcgca cgtaggcctg cgacccaggg tcgtcgccga cgagaatcgt
     3481 gcccagcccg ggggtgcgcc ccgctgcggc cagcgcctgc acgcgcttcg tcaggtcggc
     3541 gaagatctcg tcgcgggtgg ccttgccgtc gagcattgtc gccgtcacga cgccagtctg
     3601 acatgcatgt ggcaggctcc ggtctatggc tcacgacgtg tttgcccggc catgaatcag
     3661 gcgctgacgc atgggctgcc ggccgtggtc gtggccgccg cgctgtacgg gtgcgcaccg
     3721 gtgctgcagg cggcatcggt tcgacgcgcc gcgcccgccg tcgggctggg cctgcggcta
     3781 ctccccaggc tcgcggtgca accgctgtgg ctgctcggaa cagcgtgtgg gatagcggct
     3841 ttcgcggccg aagcatacgc attctcagtg gcgcccgcgg ccctcgtcgc tccgttggtc
     3901 accctcgata tggtctttct cgtcctgttg gccaggcacg ggctcagtga gcgtctgggc
     3961 atcgccggag cacttggaat ccttgcgatg gtgctcggca ccgcattgat cgcgttcgca
     4021 ttcagcggtg acgcagagct gggggcgcca gcctcgcacg cacagctgct cgcgttcctc
     4081 gtcgggggca ccgcggcggc tggcgccgcc gcggtcgtcg gggaccgcgg gagccgcacc
     4141 gggcgatcgt ggttggcggc catcgggttc ggggtcgcct ccggcgtcgc ttccgggatc
     4201 gcgaccctga ccacccgcca gatcggtctc acattcgacg tgcacgaccc ctggcccgtc
     4261 ctggccacgc cgacgccata cgcgcttctg gtggcttcaa tcctcgctct tgccttgctg
     4321 cagcgtggtt tacagtccgg gccgtcggtc ctcaccttcc cggttatgag cttcatgtcc
     4381 gcgttcgtcc ccgtgatgat cggtatcacg gtgctcgacg accaggtacc caccagtggg
     4441 ccgggcgttg gattcgtctt cgcattgctg gcggtcgctc tcggggtggc gttgctcgcc
     4501 cgcgaccgcg ccgcagccga gcacaccatg gaaaggactg acctacgcgt gggggaaacg
     4561 gcgtaacgcg agttgcccgt ttgcgctcgg aaacacctgc ccgaaccgct ctctcatcag
     4621 gtcaccgact cgcccggcca cggcatccag agacaccagc cgctcgtgcc ggcccgcgcg
     4681 ctcccgcgcg aacgggacca ctgcgtaagc cgcgatttct cccttggtcg cggcatcggc
     4741 gctgatgtcg acggcaagga tcccgccaag ccgcagcggc cacctcactc gcggcagcgc
     4801 ggtccccagg aagtcgccga ccccgaaggc aacgagcgca tcgcggatcc gctcgaccgg
     4861 ctgcagcaca tgggcgtgat gcccgacgat cagtccggcc ccgctctcgg cgaggtcgtg
     4921 cgcgagcgcg cgcgtggcgg ggcggggaga gtgccggaac tcgtaatccc aatgcgcgac
     4981 aacgcaaagc aggtcggcgc gcgagtgatg caacggcgcc gcgtcgtcgt cgagcaggtc
     5041 ctcgaggtcg gtcacacgtc cggcaaaggc tgccttgctc gcgttgcgcc attgggtgaa
     5101 ggcagcgaag ccgactgtga ggccgtcgag ttcgatagtt cgcacgagcc cgtcgtcgct
     5161 gccgccgatc gtcgcgatgc cgagacgcga aaggtgctct ctggtttcgg cgtaaccgtt
     5221 cacccctggt cgagcatgtg gttgttcgcc agcgacagca ccaaccgctg cggctcgatg
     5281 ccggcagcct ccagtgccga agcaacgaag cgaggcgaca tggtgtggcg tgtgccgagc
     5341 agtcgccggc cccgctcg
//
LOCUS       DASWBF010000047         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_594453,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000047 DASWBF010000000
VERSION     DASWBF010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594453"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1233)
                     /locus_tag="VF874_01330"
     CDS             complement(55..1233)
                     /locus_tag="VF874_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9174775.1"
                     /translation="MEEVQEFRAEVRAWLAEHLVGEFAELKGLGGPGREHEAFEERRA
                     WNQQLAAAGLTCLGWPEQHGGRGLSVAHRVAFYEEYALADAPAKVNHFGEELLGPTLI
                     AYGTPEQQQRFLPKILDVTELWCQGYSEPGAGSDLANVSTTAELDGDQWVINGQKVWT
                     SLAQHAQWCFVVARTERGSKRHAGVSYLLVPLDQAGVEVRPIVQLTGDSEFNEVFFDD
                     ARTDADLVVGEPGDGWRVAMGTLTFERGVSTLGQQIVYSRELSGIAELAQRNGAADDP
                     LIRERLARAWVGLRAMRSYALATMDVERRRSRMAGDIYKGQDNVSKLLWANWHRDLGE
                     LAMDVLGKAGMALKDGDFDEWQRLFLFSRADTIYGGSNEIQRNIIAERVLGLPRQGRR
                     "
     gene            1282..2066
                     /locus_tag="VF874_01335"
                     /pseudo
     CDS             1282..2066
                     /locus_tag="VF874_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379012.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
BASE COUNT          320 a          769 c          646 g          331 t
ORIGIN      
        1 ccgtcaccac cacgaccttg ccgtcgagca gcccccgccc agccggcccc gggctcaccg
       61 gcgcccctgc cggggcaggc cgagcacccg ctcggcgatg atgttgcgct ggatttcgtt
      121 cgaaccgccg tagatcgtat ccgcgcgcga gaacaggaac agccgctgcc actcgtcgaa
      181 atcgccatct ttcaaggcca taccggcttt gccgagaacg tccatcgcca gctcgccgag
      241 atcgcgatgc cagttggccc acaacagctt cgacacgttg tcctggcctt tgtagatgtc
      301 gccggccatg cggctccggc gtctctcgac atccatcgtg gccagcgcat acgacctcat
      361 cgcccgcaag ccgacccacg cccgggccaa ccgctcgcgg atcagcgggt cgtcggccgc
      421 accgttgcgt tgcgccaact ctgcgatgcc ggaaagctct cgcgaataga cgatttgctg
      481 cccgagcgtc gacacgccgc gctcgaacgt cagcgtgccc atcgcgacgc gccagccgtc
      541 acccggctcg ccgaccacca gatccgcgtc ggtgcgagcg tcgtcgaaga acacctcgtt
      601 gaattccgag tcgccggtga gttgcacgat cggacgaacc tcaacccccg cctggtccag
      661 cggcacaagc agatacgaca ccccggcatg acgcttggag cccctctccg tgcgggccac
      721 cacgaagcac cactgcgcgt gctgcgccag cgaggtccac accttctgcc cgttgatcac
      781 ccattggtcg ccgtcgagct cggccgtcgt ggagacgttc gccaggtcac tgccggcacc
      841 cggctccgag tagccctggc accacagctc ggtgacgtcg aggattttcg gcaggaaccg
      901 ctgctgctgc tcaggcgtcc cgtacgcgat cagggtcggg cccagcagct cctcaccgaa
      961 gtggttgacc ttggccggcg cgtcggcgag cgcgtactcc tcgtagaacg cgacgcggtg
     1021 cgccaccgag aggccgcgcc cgccgtgctg ctcgggccag ccgaggcagg tcagcccggc
     1081 ggcggccagc tgctggttcc acgcgcgccg ctcctcgaac gcctcgtgct cgcggcccgg
     1141 gccgcccagc cctttgagct cggcgaactc gccgaccagg tgctcggcca gccaggcgcg
     1201 tacctcggcc cggaactcct ggacctcttc cacccctgta ggctaaccta ccaagcactt
     1261 gctttgttag aggagcagtc gatgacgacc gatcgcaccg ttccggcggt gctggagcgg
     1321 atcgcgcgcc aattacccga ccacgacgcg ctggttgcgc cggatcgccg gttcacgttc
     1381 gcccagctgc gcgacgaggt gcgccgcgcc gccggcgcga tgatcgcgat tggcgtcgcg
     1441 ccgggtgacc gggtcgcgat ctggtcgccg aacacctggc actgggtggt ggcgtgcctg
     1501 gccgtgcacc atgccggcgc cgtgatggtg ccgctcaaca cccgctacac cgctgaggaa
     1561 gccaccgaca tcctggcccg caccggtgct cccctgctga ttgcgatggg tccattcctg
     1621 ggtgccgatc gcgtcaaggc cctgcccctc gcgaagctgg ccgcgcttcg ccacgtcgta
     1681 cgcatccccg ttgaggggcc ggtatcggcg atcgaactgg cacccgcgcg ggtcatggac
     1741 tgggacacgt tcgtctccgc cgcggacgac gcggacctag gagccgtcga cacccgtgca
     1801 gccgcggtga gcgccgacga cgtcagcgac atcttgttca cgtccggcac caccggacgc
     1861 agcaaaggtg tgttgtgcgc gcaccggcag tcgctgtcgg cgtcggcggc gtgggcggcc
     1921 tgcgggaaga tcagcagcga cgaccgctat ctgtgcatca acccgttctt ccacaacttc
     1981 ggctacaagg ccggcatcct ggccctgcct gcagaccggc gccacgctga tcccgcaact
     2041 gaccttcgac tccgagcagg cgttgg
//
LOCUS       DASWBF010000048         7041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_791723,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000048 DASWBF010000000
VERSION     DASWBF010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791723"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VF874_01340"
     CDS             <1..521
                     /locus_tag="VF874_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174776.1"
                     /translation="KRTALALNHLVSTKFRSDALQIIAFGRYARTMSAAELTGLEGVY
                     EQGTNLHHALALAARHLRRHPSAQPVVLVVTDGEPTAHLEGGFGRADEPAQVFFDYPP
                     HPRTIAHTVKGFDEVANLGAQVTIFRLGNDPGLARFIDQVARRVQGRVVVPDLDGLGA
                     AVVSDYLRSRRR"
     gene            677..1015
                     /locus_tag="VF874_01345"
     CDS             677..1015
                     /locus_tag="VF874_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX9174777.1"
                     /translation="MTRKIDPKKIRFDEDTIVEDVDLNATEIYVEGERLTDERAEQMA
                     DEVLALARAKNLRPGGKSLSGGSRHSPVVQVRVSETTRSKLQAIARDRKMSLSKLSRQ
                     VLDDFVDRNS"
     gene            complement(1072..1656)
                     /locus_tag="VF874_01350"
     CDS             complement(1072..1656)
                     /locus_tag="VF874_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX9174778.1"
                     /translation="MTSNLPRRKAELDADDTDRIQVAQLLTDAAAQGRLGMDEYEDRL
                     AKVYSARTYRELDRLSSDLPGASMRPRRHGGACRPAPSTTLLAIMSGFERRGRWNVPR
                     RLTTFALWGGGVVDLRYADFTCPDVEIKSYSIMGGQTIVVPPEVNVEIQGRGVMGAFD
                     HSAAGNGMPGAPHVVVKGFSLWGSVGVRRKQRKH"
     gene            complement(1702..2457)
                     /locus_tag="VF874_01355"
     CDS             complement(1702..2457)
                     /locus_tag="VF874_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX9174779.1"
                     /translation="MTRTVLVTGATGTLGREVLPLAVAAGHRVRALSRHDHPDSDVRW
                     HRCDLLTGDGVAEAVDGVDVVLHCATQPTGGKDVTSTQNLLGAAQRAGVGHLVYISIV
                     GVDRIPLPYYKTKLRVEQVLAASHVGHTVLRATQFHDLIAAIFSAQRLSPVLFALRGV
                     DFQPIDTRDVAARLVELGDEQAAERVPDIGGPEVRSHADLGRAYLRWRGSRRPVVAVP
                     VPGTIAASYRAGAHLAPQTPVGTVRFEDYLRRS"
     gene            complement(2478..3215)
                     /locus_tag="VF874_01360"
     CDS             complement(2478..3215)
                     /locus_tag="VF874_01360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9174780.1"
                     /translation="MRYRFISWDHWTLAVLKCRMWLGDARACRVEKPSKLALLRAGEV
                     GEHPRSIGEPQPELAAVIDQGKFHGDVLDVGCGEAAVSLRLAELGYTTVGLDLSPTAI
                     ELARAEAAKRGLTNATFEIADISSFDAYDGRFGTIVDSTLFHSIPVELRNGYQRSIVR
                     AAAPGASYFVLVFDRAGMPEGPANPVTADELRDVVSKYWTIDEIKPARIHANVPDDFT
                     AMGEVELRDEPGGRKSMRAWLLSAHLS"
     gene            complement(3302..4051)
                     /locus_tag="VF874_01365"
     CDS             complement(3302..4051)
                     /locus_tag="VF874_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX9174781.1"
                     /translation="MYVDYAVEGSTARVTLNSPHNRNALSSTLVEELHQGLRDAAADM
                     KVRAVVLGHTGGTFCAGADLSEASGDSKDRTRQMTELLRAILELPLPVVGAIDGHVRA
                     GGFGLVGACDIVVAGRNSTFALTEARIGVAPAIISLTLLPKMTPRSVGRYFMTGEKFG
                     AEEAERIGLVTIAADDVAAAVARITAELGKGSPQGLRESKALTTAAILDGFDQRAQEL
                     SAQSARLFASDEAREGMLAFLQKRPPSWVAD"
     gene            complement(4051..5217)
                     /locus_tag="VF874_01370"
     CDS             complement(4051..5217)
                     /locus_tag="VF874_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9174782.1"
                     /translation="MTDNGFIENEERQALRKSVAAWAANYGQDYYLEKARSGQHTDEL
                     WSEAGKLGFLGVNLPEEYGGGGAGMYELSLVMEEMAAQGCALLLMVVSPAINGTIISK
                     FGTDEQKQRWLPGIADGSITMAFAITEADAGSNSHRISTTARKDGSDWILNGQKVYIS
                     GVDQSQAVLVVGRTEEAKTGKLKPALFVVPTDTPGFTYTPIEMELISPERQFQVFIDN
                     VRLPADALVGSEDAAIAQLFAGLNPERIMGAASSVGLGRFAIGKAVDYVKTRQVWKVP
                     IGAHQGLSHPLAQNYIEVELARLMMQKAATLYDSGDDVGAAEAANIAKYAAGEASTRA
                     VDQAVQCLGGNGLTKEYGIAAAITASRLARIAPVSREMVLNFVAQTSLGLPRSY"
     gene            complement(5214..>7041)
                     /locus_tag="VF874_01375"
     CDS             complement(5214..>7041)
                     /locus_tag="VF874_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9174783.1"
                     /translation="DARVPLPGPNGYLNGAALIDAAKAAGADAIHPGYGFLSENAEFA
                     AAVQDAGLTWIGPPVPAVAAMGSKIEAKKMMAAAGVPVLDELDPASVTESQLPVLVKA
                     SAGGGGRGMRVVAELSKLPPEVAAAQREAASAFGDDTVFCERYLPRGRHIEVQVLADE
                     HGNVWAVGERECSIQRRHQKVIEEAPSPLVERIPGMRDKLLDAARQAAAAIGYAGAGT
                     VEFLADDDGSFYFLEMNTRLQVEHPVTECVTGLDLVGLQLQVADGIRLDPEPPVAHGY
                     SIEARLYAEDPARGWQPQAGTLRRFEIPTAQAAFVPLTRTGVRLDSGVVDGSVVSAHY
                     DPMLAKVISYAPTRTQAAALLADALSRARVHGVRTNRDLLVNVLRHPAFVAGDTDTAF
                     FDTHDLASLSKPLADADAVPWSALAAALADAAHNRATATVLGSVPSGWRNLASGFQVK
                     RYADVDGDEYEVRYRIERGALRVPDTDVRLVSATPDQVVLRVDGVDRPFDVARYDDSV
                     FVDSPSGPVQLAVVPRFPEPGSALQPGSLVAPMPGAVTRLGAAVGDTVTAGQPIVWME
                     AMKMEHAVMSPKDGVVAELSVEVGQQVELGAVLARVESKEES"
BASE COUNT         1095 a         2444 c         2368 g         1134 t
ORIGIN      
        1 tgaagcgcac cgcgttggcg ctgaatcacc ttgtcagcac gaaatttcgg tcggacgcgt
       61 tgcagatcat cgcattcggc cgatacgcgc gcacgatgag cgccgccgag ctgaccgggc
      121 tcgagggcgt ctacgagcag ggcacaaacc tgcatcacgc gctggctctg gccgcacggc
      181 atctgcgccg gcatccgagc gcgcagccgg tcgtgctggt ggtcaccgac ggcgagccga
      241 ccgcgcacct ggagggcggc ttcggccgcg ctgacgagcc ggcgcaggtc ttctttgact
      301 acccgccgca tccgcggacg atcgcgcaca cggtgaaggg ctttgacgaa gttgccaacc
      361 tcggcgcgca ggtgacgatc ttccggctgg gcaacgaccc cggcctggcg cggttcatcg
      421 accaggtggc gcggcgggtg cagggccgcg tcgtcgtgcc cgacctcgac gggctgggcg
      481 cggcggtggt cagcgactac ctgcgctcac gccgccgcta accctctttt ccttttccgc
      541 gagcgtgcat gtttgtagcc ctacgccgca gaatggggta caaacgcatg ctcgccggcg
      601 tggtcgacga ttacgacgcc gacgtctgga ttgctatcca cgccatgccc attcactatc
      661 ggaagaacat cgagtcatga cacgcaagat cgaccccaag aagatccgct tcgacgagga
      721 caccatcgtc gaggacgtgg acctcaacgc aacggagatc tacgtcgaag gcgagcgcct
      781 gaccgacgag cgtgccgagc aaatggccga cgaagtcttg gcactggccc gcgcgaaaaa
      841 ccttcgcccg ggcggcaagt cactatccgg cggaagcagg cattcgccgg tggtacaagt
      901 gcgggtctcc gaaacgacta ggtccaagct gcaagcgatc gcccgcgacc gcaagatgag
      961 tttgtcgaaa ctgtcccgtc aggtcctaga cgacttcgtc gaccgcaaca gctagagagt
     1021 gcgcccgcta ccctcgcgaa cggtgatggc gggaggagcg cgcagaacgc gtcagtgctt
     1081 gcgctgcttg cgcctgaccc cgacgctgcc ccacagcgag aagcccttga cgacgacgtg
     1141 cggcgcgccg ggcatcccgt tgcccgccgc gctgtggtcg aaggcgccca tcacaccgcg
     1201 gccttgaatc tcgacgttga cctccggcgg caccacgatg gtctggccgc ccatgatcga
     1261 gtacgacttg atctcgacgt cgggacacgt gaagtccgcg taccgcaggt cgacgacgcc
     1321 gccgccccac agcgcgaacg tcgtcagccg tctcggcacg ttccaccggc ctcggcgctc
     1381 gaagccgctc atgatcgcca gcagagtcgt cgacggcgcc ggacggcagg caccgccgtg
     1441 gcgtcgcggc cgcatcgacg cgcccggcaa atcggagctg agccggtcca gctccctgta
     1501 ggtcctcgca gagtagacct tcgctaatcg gtcctcgtac tcgtccatcc cgagccggcc
     1561 ctgggcagcg gcgtcggtca gcaactgcgc gacctggatg cggtcggtgt cgtcggcgtc
     1621 caactccgct tttcggcgag gcagattgct ggtcatcaat gtcgagcgta cggcgttgcc
     1681 gtggcgctgc ccagaaaccc gtcaagaacg gcgaaggtag tcctcgaacc gcacggtgcc
     1741 gaccggggtc tgcggcgcca ggtgcgcacc cgcacggtag ctcgcggcga ttgtgccggg
     1801 caccggcacc gccaccaccg gccgccggct gccgcgccac cgcaggtaag cccgcccgag
     1861 gtcagcgtgg cttcggacct caggtccgcc gatgtcaggg actcgttcgg cagcctgctc
     1921 gtccccgagt tcgactaggc gggcggccac gtcgcgggtg tcgatcggct ggaagtccac
     1981 tccgcgcagc gcgaacagca ccggtgagag ccgctgcgcc gagaagatcg cggcgatcag
     2041 atcgtggaac tgcgtggccc gcagcacggt gtggccgacg tgcgaggcgg ccagcacctg
     2101 ctcgacgcgc aacttggttt tgtagtacgg cagcgggatg cggtccactc cgacgatcga
     2161 gatgtacacc aggtggccga cacctgcccg ctgcgccgcc ccgagcaaat tctgcgtcga
     2221 cgtgacgtcc ttgccgccgg tcggctgcgt cgcacaatgc agcacgacgt cgacgccgtc
     2281 gacggcctca gccacgccgt cgccggtgag caagtcgcag cggtgccagc gcacgtcgct
     2341 gtcgggatgg tcgtggcggc tcagcgcgcg aacccggtgc cccgccgcga ccgcgagcgg
     2401 cagcacctca cgccccaaag ttccggtggc cccggtcacc agcacggtgc gcgtcacggc
     2461 ctgagcctag gtgggcgtca gctcaggtgc gccgacaaca accatgcccg catcgacttg
     2521 cggccgcccg gttcgtctct cagctcgacc tcgcccatgg ccgtgaagtc gtcgggcacg
     2581 ttcgcgtgga tgcgggcggg cttgatctcg tcgatagtcc agtacttcga caccacgtca
     2641 cgaagctcgt cggcggtgac gggattggcg gggccctccg gcatgcctgc ccggtcgaag
     2701 acgagcacga agtacgacgc acccggcgcc gcggcgcgga cgatcgagcg ctggtagccg
     2761 ttgcgcagct cgaccggaat cgagtggaac agcgtgctgt cgacgatcgt gccgaacctg
     2821 ccgtcgtacg cgtcgaacga gctgatgtcg gcgatctcga aggtcgcgtt ggtgagtccg
     2881 cgcttggcgg cctcggcccg ggccaactcg atggcggtcg gcgaaaggtc cagtccgacc
     2941 gtggtgtagc ccaactccgc cagccgcagc gacaccgcgg cctccccgca gccgacgtcg
     3001 agcacgtcgc cgtgaaactt gccctggtcg atcaccgcgg cgagctcagg ttgcggttcg
     3061 ccgatgctcc gggggtgctc gccaacctca cccgcgcgga gcaacgcgag cttcgacggc
     3121 ttctcgacgc ggcaggcgcg agcgtcgcct agccacatac ggcacttcag caccgccaat
     3181 gtccaatgat cccaactgat aaagcgatat cgcatgcgat atcgctttcg tgaccgttgt
     3241 tggtagaggt gtggcggtgc tggtgtggct ggtgcggcca gcgttgctaa tccggttgct
     3301 tctagtcggc cacccagctc ggcggccgtt tctgcaggaa cgcgagcatg ccctcgcgcg
     3361 cctcgtcgga ggcgaacagt cgggccgact gcgcgctcag ttcctgcgct cgctggtcga
     3421 agccgtccag gatcgcggcc gtcgtcagcg ccttggattc gcgcagcccc tgcggcgagc
     3481 ccttgccgag ttcggcggtg atccgcgcga cggccgcggc cacgtcgtcg gcggcgatcg
     3541 tcaccagccc gatccgttcc gcctcctcgg cgccgaactt ctcgccggtc atgaaatagc
     3601 ggccgaccga ccggggagtc atcttcggca acagcgtcag cgagatgatc gccggtgcga
     3661 cgccgatgcg cgcctcggtg agcgcgaagg tgctgttgcg gcccgcgacg acgatgtcgc
     3721 aggcgccgac gagcccgaaa ccgccggcgc gcacgtgccc gtcgatggcg ccgacgaccg
     3781 gcagcggcag ttcgaggatg gcccgcagca gttcggtcat ctggcgggtg cgatccttcg
     3841 agtcgccgct cgcctccgac aggtcggcgc ccgcgcagaa cgtgccaccg gtgtggccga
     3901 gcaccaccgc ccgcaccttc atgtcggcgg ccgcgtcccg cagcccttga tgcaactcct
     3961 cgacgagcgt cgacgacagt gcgttgcggt tgtgcggcga gttcagcgtg acccgcgccg
     4021 tcgacccttc gaccgcgtag tcgacgtaca tcagtaggag cgcggcagac cgagcgaggt
     4081 ctgggcgacg aagttcagca ccatctcgcg gctcaccggc gcgatacggg ccagccgcga
     4141 cgcggtgatc gccgccgcga tgccgtactc cttcgtcaaa ccgttgccgc ccaggcactg
     4201 aacggcctga tcgaccgcgc gggtcgacgc ttcaccggcc gcgtacttgg cgatgttcgc
     4261 cgcctccgcg gcgccgacgt cgtcgccgct gtcgtacagc gtggcggcct tctgcatcat
     4321 cagcctggcc agctcgactt cgatgtagtt ctgcgccaac ggatgtgaca ggccctggtg
     4381 cgcaccgatc ggcaccttcc acacctgccg ggtcttgacg tagtcgacgg ccttgccaat
     4441 ggcgaagcgg cccaggccca ctgagctcgc cgcccccatg atgcgctcag ggttcaaccc
     4501 agcgaacagc tgcgcgatcg cggcgtcctc cgagccgacc agagcgtccg cgggcagccg
     4561 gacgttgtca atgaacacct ggaactggcg ctccgggctg atcaactcca tctcgatcgg
     4621 cgtgtaggtg aagccgggag tgtcggtggg cacgacgaac agcgcgggct tcagctttcc
     4681 ggtcttggcc tcctcggtgc ggccgaccac cagaaccgcc tgcgactggt cgaccccgga
     4741 gatgtacacc ttctggccgt tgaggatcca gtcgctgccg tctttgcggg ccgtcgtgga
     4801 gatgcggtgg ctgttcgacc cggcgtcggc ctcggtgatc gcgaatgcca tcgtgatcga
     4861 cccgtcagcg atgcccggca gccagcgctg cttctgctcg tcggtgccga acttgctgat
     4921 gatcgtgccg ttgatcgccg gcgacaccac catcagcagc agcgcgcagc cttgcgcggc
     4981 catctcctcc atcaccagcg acagctcgta catgccggcg ccgccgccgc cgtactcctc
     5041 gggcaggttc accccgagga agccgagttt gcctgcctcg ctccataatt cgtcggtgtg
     5101 ctggccgctg cgggcctttt cgaggtagta gtcctggccg tagttggcgg cccacgctgc
     5161 caccgacttg cgcagcgcct ggcgttcctc gttctcgatg aagccgttgt cggtcatgat
     5221 tcctccttgc tttcgacgcg ggcgaggacg gcgcccagtt cgacttgttg gcctacttcg
     5281 acggaaagtt cggctactac accgtctttg ggcgacatca cggcgtgctc catcttcatc
     5341 gcctccatcc agacgatcgg ctgaccggcg gtgacggtgt cgcccaccgc ggcgccgagc
     5401 cgggtgaccg cgccgggcat cggcgcgacg agcgagccgg gctgcaacgc cgagcccggt
     5461 tccgggaagc gcggcacgac cgccaattgc acggggccgc tgggtgagtc gacgaacacg
     5521 ctgtcgtcgt agcgcgcgac gtcgaacggc cggtcgacac cgtcgacgcg cagcaccacc
     5581 tggtcaggcg tcgccgacac gagccgtacg tcggtgtcgg gcactcggag cgctccgcgt
     5641 tcgatccggt accgcacctc gtactcgtcg ccgtcgacgt ctgcgtagcg cttgacctga
     5701 aagcccgatg ccaggttgcg ccagcccgac ggcacgctac ccaaaaccgt cgcggtggcg
     5761 cggttgtggg cggcgtcggc gagtgccgcc gccagcgcgg accagggcac ggcatcggcg
     5821 tcggccaacg gtttggacag cgacgcgagg tcgtgggtgt cgaagaacgc cgtgtcggtg
     5881 tcgccggcga cgaacgccgg gtgccgcagc acgttgacca gcagatcgcg gttcgtgcgc
     5941 accccgtgca cgcgggcacg cgagagtgcg tcggcgagca gcgcggccgc ctgagtacga
     6001 gtcggggcgt aggagatgac cttggccagc atcgggtcgt agtgggcgga caccaccgat
     6061 ccgtcgacga cgccggagtc cagccggaca ccggtgcggg tcagcgggac gaacgcggcc
     6121 tgcgcggtag ggatctcgaa tctgcgcagc gtgcccgctt gcggctgcca cccgcgcgcc
     6181 gggtcctccg cgtacagccg cgcctcgatc gaatagccgt gagcgacagg cggttccggg
     6241 tccagacgga tgccgtcggc gacctgcagc tgcagtccga cgagatcgag accggtgacg
     6301 cattcggtga ccgggtgctc gacctgcagc cgagtgttca tctccaggaa gtagaacgat
     6361 ccgtcgtcgt cggcgaggaa ctccacggtg cccgcgccgg cgtagccgat cgccgccgcc
     6421 gcctgacggg cggcgtcgag cagtttgtcg cgcataccgg ggatgcgttc gacgagcggc
     6481 gacggcgcct cctcgatcac cttctggtgg cgccgctgga tcgagcactc gcgttcgccg
     6541 accgcccaga cgttgccgtg ctcgtcggcg agcacctgca cctcgatgtg acggccgcgc
     6601 ggcaggtaac gctcgcagaa cacggtgtcg tcgccgaacg cggatgccgc ttcgcgctgc
     6661 gcggcggcga cttcgggtgg cagcttggat aattcggcga cgacgcgcat tccgcggcca
     6721 ccgccgcccg cggacgcctt gacgagcacc ggcagctgcg actcggtgac gctggcgggg
     6781 tcgagctcgt cgagcaccgg cacgcccgcc gcggccatca tcttcttggc ttcgatcttg
     6841 ctgcccatcg ccgccaccgc gggcaccggc gggccgatcc aggtcaaccc cgcgtcttgc
     6901 acggcggctg cgaattcggc attctccgaa aggaatccgt aacccgggtg gatggcgtcg
     6961 gcgccggcgg ccttcgccgc gtcgatcaga gcggcaccgt tgaggtaccc gttggggccg
     7021 ggcagcggca cgcgcgcgtc g
//
LOCUS       DASWBF010000049         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_594604,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000049 DASWBF010000000
VERSION     DASWBF010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594604"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF874_01380"
     CDS             complement(<1..256)
                     /locus_tag="VF874_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174784.1"
                     /translation="MTTVNGQLLLVLIDASESSGHLVSLLGSPSSDGISWSWPPSPVG
                     PPDRPSASLVDPGLGVLDGTIYCVYTQGDGGLRFVTSRDGT"
     gene            356..1384
                     /locus_tag="VF874_01385"
     CDS             356..1384
                     /locus_tag="VF874_01385"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9174785.1"
                     /translation="MRDNDGRKLDHATLEVLRMRAVDRIRAGASVAEVAETLGLHRGS
                     VYRWWWRYLQGGTEALRAKPVPGRPATLTDEQLARLREIVAYCDPRQLQFDFGLWTRD
                     MIAAVVQREFGIRLHPSTIGRILRRLGFSPQRPLHRAYQQNPEAVQTWKTETFPKIRK
                     QAKKEKAAIYFADEAGVRSDYHSGTTWAPRGETPIVRTTGARHRLNMLSAVTATGVLR
                     FMIREGGVNAQVFIEFCKRLLADADGPVYLVVDGHPAHRAKVTRTFVASTGGRLKLFF
                     LPGYSPELNPDEWVWKNIKYDRVGKTPITDGNHFKTVVISALHRLQKQVNKIRGFFAD
                     PNLAYITA"
     gene            1421..1915
                     /locus_tag="VF874_01390"
     CDS             1421..1915
                     /locus_tag="VF874_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174786.1"
                     /translation="MATLGHHYVDDSLRNTFTEASIVTRVIAELFATRFDKQLVAGVA
                     PEPGSVLAAHVSRLASACERRKLAAALHHSVCEAHSRGRRMSATIPVDRSAVAAAVGL
                     IDHVVLRLTGPRPVSARGVARLRLLLSDRRRADVLARRWRPGRRTARCARPAVTTPIA
                     PLLV"
     gene            complement(1968..2102)
                     /locus_tag="VF874_01395"
                     /pseudo
     CDS             complement(1968..2102)
                     /locus_tag="VF874_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          351 a          724 c          696 g          337 t
ORIGIN      
        1 cggtgccgtc gcgactggtc acgaaccgaa ggccgccatc cccctgtgta tagacgcagt
       61 agatcgtgcc gtccaacacg ccaaggcccg ggtccaccaa agacgcagat gggcggtcgg
      121 gggggccgac agggctcggc ggccacgacc aggagatgcc gtcgctgctg ggactgccga
      181 gcaggctcac cagatgtccg ctcgactccg acgcgtcgat taggacgagg agcagctgac
      241 cgttgaccgt tgtcaacgct gggctgctca gcgtcttact aaatcgagca taagtaaggc
      301 gacacgccgg gcgtgatgct cgagttcgct gagcgtctcg tgttggctaa ctgcagtgcg
      361 ggacaacgac ggtcgcaagc tcgaccatgc cacgctcgag gtgctgcgga tgcgggcagt
      421 ggaccggatc cgggccggcg cgtcggttgc ggaggtggcc gagacgcttg gcctgcaccg
      481 tggttcggtg taccgatggt ggtggcggta cctgcagggc gggaccgagg cgctgcgggc
      541 caagccggtg cctgggcggc cggccacgtt gaccgacgag cagttggcac ggctgcgcga
      601 gatcgtcgct tactgcgacc cgcggcagct gcagttcgac tttgggctgt ggacccgcga
      661 catgatcgcg gctgtcgtgc agcgcgagtt cgggattcgg ctgcacccga gcaccatcgg
      721 ccggatcttg cgcaggctcg ggttctcccc gcaacgcccg ctgcaccggg cctaccagca
      781 gaaccccgag gcggtgcaga cgtggaagac cgagaccttc cccaagatcc gcaagcaggc
      841 gaagaaggag aaggcggcga tctacttcgc cgacgaagcc ggggtgcgtt cggactacca
      901 ttccggcacc acctgggccc cgcgcggcga gacgccgatc gtgcgcacca ccggcgcccg
      961 gcatcggctg aacatgctct cggcggtcac cgcaaccggc gtgctgcggt tcatgatccg
     1021 cgaaggcggc gtgaacgcgc aggtgttcat cgagttctgc aagcggctgc tcgccgatgc
     1081 cgacggtcct gtctacctcg tcgtcgacgg ccaccctgcc caccgcgcca aggtgacccg
     1141 cacgttcgtg gcctccaccg gcgggcggct gaaactgttc ttcctgcccg gctactcgcc
     1201 cgagctcaat cccgacgaat gggtttggaa gaacatcaaa tacgaccgtg tcggcaagac
     1261 cccgatcacc gacggcaacc actttaagac cgtcgtgatc agcgccctgc accgactcca
     1321 aaagcaggtc aacaagatcc gcggattctt cgccgacccc aacctcgcct acatcaccgc
     1381 gtgagtcgcc ttactaacgc tcgccttggt aattcgcggg atggccaccc tagggcatca
     1441 ctatgtcgac gactcgctgc ggaacacctt caccgaggcg tcgattgtta cgcgtgtgat
     1501 cgcggagctc ttcgcgaccc ggttcgacaa gcagctcgtg gccggcgtgg cacccgagcc
     1561 cggcagcgtc ctcgctgcgc acgtgtcacg attggcgtcg gcctgcgagc gacgcaagct
     1621 cgcggcagcg ctgcaccaca gtgtctgcga ggcccactcg cgcggtaggc ggatgtcggc
     1681 aacaatcccc gtcgaccggt cagccgtcgc agccgcggtg ggtttgatcg accacgtcgt
     1741 gctgcgcctg accggtccgc gtccggtgag cgcgcgcggt gtcgcgcggc tgcgcctgct
     1801 gctgtccgac cggcgccggg ccgatgtact ggcccggagg tggcgacctg ggcgccgaac
     1861 tgcgcgctgt gctcgcccag ctgtgacgac accgatcgca ccattgctgg tttagatgcc
     1921 tcgcgacggg ggcagcgact gttgatgcca gcggttatgc gcgccgacga cacaatgcgc
     1981 cgcaccgcgt tggtggacca ccgcgccccc cgcaccgtgg gcaccccgga atcgttgagc
     2041 cacaccgtca gcgcgcgcac cgattccccg gcgatcaccc gccccactag ctcgcgcacc
     2101 accgcggc
//
LOCUS       DASWBF010000050         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1252378,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000050 DASWBF010000000
VERSION     DASWBF010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252378"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VF874_01400"
     CDS             complement(<1..367)
                     /locus_tag="VF874_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9174787.1"
                     /translation="MPPPGVIREFVGLPSPTARRGGAGGHPCQGLYHRGAGRKPKVAM
                     IATHYQIDFSEHYLADYMATRGIGFLGWNTRFRGYESNFLLDHALVDIGVGVRWLLQV
                     QGVETVVLLGNSGGGSLMAG"
     gene            complement(369..1010)
                     /locus_tag="VF874_01405"
     CDS             complement(369..1010)
                     /locus_tag="VF874_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9174788.1"
                     /translation="MADQGVIVAQPKQPLPTVRGRQTQAAIDAAARTVIARKGILATT
                     ISDIAKQARRSTASFYNYYDSKESMVREWALRFRDEANERAKSVTRHGLSNRERAREA
                     AAAHWHTYRHRTAEMISVSQLAIVNDDFAEYWAELCAIPISFITTMIERAQSEGYCPD
                     DDPHLLAVAIVSMLNQFCYVQLSGRDPDDVDDDACITTLANIFYRAIFCKEEA"
     gene            1078..1668
                     /locus_tag="VF874_01410"
     CDS             1078..1668
                     /locus_tag="VF874_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9174789.1"
                     /translation="MIKPQNTNTEFALSGINHVALVCSDMARTVDFYSNVLGMPLIKA
                     LDLPGGVGQHFFFDAGNGDCVAFFWFRDAPDAVPGISAPRHIPGIGDIVSAVSSMNHL
                     SFHVPAEKFDEYRQKLKAKGVRVGPVLNHDESESQVAAQLHSGVYVRSFYFHDPDGIT
                     LEFACWVKEFGQADVSTEPKTAADRRVPAQVSPAGG"
     gene            complement(1649..2257)
                     /locus_tag="VF874_01415"
     CDS             complement(1649..2257)
                     /locus_tag="VF874_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9174790.1"
                     /translation="MSARPYRGVKPADRQAQRRARLLDAGLDILGSGSNPEALTVRGL
                     CRQANISARYFYESFTDRDEFVGDVCDWVIADIATTTQAAVAATSPDGQARAGMASIV
                     RSIAGDVRVGRLLFGTELADPVVVRKRSESAALLAALLGQHGQQALGLPDDERLKATS
                     QFVVGGVGQALTAWLTGQVALTQDELIDQLAWFIDRLSRQRG"
     gene            2339..3226
                     /locus_tag="VF874_01420"
     CDS             2339..3226
                     /locus_tag="VF874_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX9174791.1"
                     /translation="MTTTESLAHVERPVSTPPARPAYRRRRGTDPGMLGIGLLAGPAN
                     VIMQLARPAIGYGVKDSRVESGRVDRHPIKRARTTFTYIAVAISGTEDQKAAFRRAVN
                     RAHAQVYSLPDDPVAYNAFDPDLQLWVAVCMYKGVVDVYRLFVGEMDDETADEHYREA
                     MVLGTMLQVPPEMWPKDRAAFDEYWRKSLELVHIDDAVRNYLYPIAAGRTAMPLPRLV
                     RTSLENISLLITTGFLPQRFRDEMRLSWDARRQRRFDKLMAALRIVNNLSPKFVREFP
                     FNVLLIDVDWRIRTGRPLV"
BASE COUNT          554 a         1067 c         1054 g          569 t
ORIGIN      
        1 accccgccat cagcgatccg ccaccggaat tccccagcaa cacaacagtt tccacgccct
       61 gcacttggag tagccagcgc acgccgacgc cgatgtccac cagcgcgtgg tcgagcagga
      121 agttgctctc gtacccgcgg aaccgggtat tccagccgag gaacccgatg ccccgggtgg
      181 ccatatagtc ggcgaggtag tgctcggaga agtcgatctg gtagtgcgtg gcgatcatcg
      241 ccaccttggg tttgcggccc gcgccgcggt ggtagaggcc ctggcacgga tggccgccgg
      301 cgccgccacg ccgcgcggtg ggcgacggca ggccgacaaa ctcacggatc actcccggcg
      361 gcggcatgtc acgcttcctc cttgcagaag atcgctcggt agaagatgtt ggccagcgtg
      421 gtgatgcacg cgtcgtcgtc gacatcgtcc gggtcgcgtc ccgacagctg cacgtaacag
      481 aactggttga gcatcgacac gatcgcgacc gcgagcagat ggggatcgtc gtcggggcag
      541 tacccctcgg actgagcgcg ttcgatcatg gtggtgatga acgagatggg gatcgcgcac
      601 agttcggccc agtattcggc gaagtcgtcg ttgacgattg ccagctgcga cacgctgatc
      661 atctcggccg tccggtggcg gtaggtatgc cagtgggcgg ccgcagcctc gcgcgcgcgt
      721 tcgcgattcg acagcccgtg ccgggtcacc gatttcgctc gctcgttggc ctcgtcacgg
      781 aagcgcagcg cccactcgcg gaccatcgac tccttggagt cgtagtagtt gtagaacgac
      841 gccgtcgacc gccgtgcctg cttcgcgatg tcggagatcg tggtcgccaa aatgcctttg
      901 cgcgcaatca ccgtgcgggc ggccgcatcg atcgcggcct gcgtctgccg gccacgaacc
      961 gtcggcagcg gctgcttcgg ttgcgccaca atcactccct ggtcggccat tgagctgaat
     1021 ctgaatctga tgttagattc agattcggct gttgagaagg tccggagggg acgcgtcatg
     1081 atcaagccgc agaacaccaa caccgagttc gcgctgagcg gcatcaacca cgttgcgttg
     1141 gtctgctcgg acatggcgcg caccgtcgac ttctacagca acgttctggg gatgccgctg
     1201 atcaaagcgc tcgacttgcc cggtggcgtc ggccagcact tcttcttcga cgcgggcaac
     1261 ggcgactgcg tcgcgttctt ctggttccgc gatgctccgg acgcggtgcc cggcatctcg
     1321 gcgccgcgtc acatccccgg catcggcgac atcgtcagcg cggtcagctc gatgaaccac
     1381 ctgtccttcc acgtgcccgc cgagaagttc gacgagtacc gccagaagct gaaggccaag
     1441 ggggtgcgag tcgggccggt gctcaaccat gacgagagcg agtcgcaggt cgcggcccag
     1501 ctgcactccg gcgtctacgt gcggtcgttc tacttccacg acccggacgg catcacgctc
     1561 gaattcgctt gctgggtgaa ggaattcggc caggctgacg tcagcaccga gccgaagacg
     1621 gccgctgacc gccgggtgcc cgcgcaggtc agccccgctg gcggctgagc cggtcgatga
     1681 accaggccag ctgatcgatc agttcgtcct gggtcaacgc cacctgcccg gtgagccacg
     1741 cggtgagcgc ctgccccacg ccccccacga cgaattgcga cgtggccttg aggcgctcgt
     1801 catccggcag ccccaacgct tgctgaccgt gctggcccag cagcgcagcc agcagggccg
     1861 cggactcgct ccgtttgcgc accaccaccg ggtcggccaa ctcggtgccg aacagcagcc
     1921 gtccgacgcg cacgtcgcct gcgatcgagc ggacgatgct ggccatcccg gcacgcgcct
     1981 gcccgtccgg ggacgtcgcg gcgacggcgg cctgcgtcgt ggtggcgatg tcggcgatca
     2041 cccagtcgca cacatctccg acgaattcgt ctctgtccgt gaaactttcg tagaagtagc
     2101 gggccgagat gttcgcttgc cggcagaggc cccgtacggt cagcgcctct ggattcgatc
     2161 cggatccgag gatgtcaagt ccggcatcca gcagccgtgc ccgtcgctgg gcctggcggt
     2221 ccgctggctt gaccccacgg tatgggcggg cgctcatacc gaatatcttg acaccctcgc
     2281 tgcccgcctg ggaacgttta ggaaacgtac gttatcagat atctggacgg gggcgcccat
     2341 gacgaccacc gagtcccttg cgcatgtcga gcggcctgtc tctacgccgc cggcaagacc
     2401 ggcgtaccgc cgccgccgag ggacggaccc cggaatgctg ggcatcgggc tgttggccgg
     2461 gccggcaaat gtgatcatgc agctggcacg tcctgccatc ggctacggcg tgaaggacag
     2521 ccgggtggaa agcggacgcg tcgatcgcca tccgatcaag cgagcccgca cgactttcac
     2581 ctacatcgcg gtcgcgatta gcggcaccga ggatcagaag gcggcgtttc ggcgggcggt
     2641 caaccgtgcc cacgcacagg tctattcact gcccgacgat cccgtcgctt acaacgcctt
     2701 cgaccccgac ctgcaacttt gggtagcggt gtgcatgtac aagggcgtcg ttgatgtgta
     2761 ccggctgttc gtcggcgaga tggacgacga gaccgctgac gagcactacc gcgaggccat
     2821 ggtgctgggc accatgctgc aggtgccgcc ggagatgtgg cccaaggacc gcgccgcgtt
     2881 cgacgagtac tggcggaagt ctctcgaact ggtgcacatc gacgacgccg tgcgcaacta
     2941 tctctatccg atcgcggccg gccgaacggc gatgccgctg ccgcggctgg tccgcacgtc
     3001 gctggaaaat atctcactgt tgatcacgac cggctttctg ccgcaacggt ttcgcgacga
     3061 gatgcggctg tcctgggatg cccgccgaca acgcaggttc gacaaattga tggcggcgct
     3121 gcggatcgtc aacaacttgt cgcccaagtt cgtccgcgag ttcccgttca acgtgctgct
     3181 gatcgatgtg gactggcgga tccggacggg acgcccgctg gtgtagggac gcggacgcga
     3241 aaca
//
LOCUS       DASWBF010000051        10724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_69673, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000051 DASWBF010000000
VERSION     DASWBF010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69673"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VF874_01425"
     CDS             <1..430
                     /locus_tag="VF874_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX9174792.1"
                     /translation="IDLTFVRGSLDDDALGRLTDELVTVLLRAERAPDTLFLRDNTLV
                     YPHALEPSALSVGGRGRGEPRFRVELTVFEGALSQDRKERLVSDVHAAVCSAAGIDPQ
                     GSTAYHVWTLIHEVPEGNWAGGGKIIYHQNVKGLVADDEP"
     gene            445..1512
                     /locus_tag="VF874_01430"
     CDS             445..1512
                     /locus_tag="VF874_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9174793.1"
                     /translation="MEGVAELVPVLPAHRFDEEALAGYLRGLPGFDGHLEICQFQGGQ
                     SNPTFHLRTAGGEYVLRKKPPGKLLPRAHEVEREHRVMSALADSDVPVPRMRLLCQDE
                     SVIGTQFFVMDYVPGRIFHDPGLRDLAPSDRAAIYEDLARVLAALHRVDWRAAGLDGF
                     GKPDGYMQRQVALWTRQWQAARVEDNPTMDRLAQWLPEHLPSDDEPACIAHGDYRLGN
                     VLIHPTEPRIVAVLDWELATIGHPLADLGYTCLTYHLPAHFIGTRGVAGEDLTGTGIP
                     DESAFVASYCRYAGRNEPASLDVFVVFSMFRLASITAGVWRRGLDGNAADSRAGTSEF
                     RDRYRALAQMAWTLAQQLSDA"
     gene            complement(1514..2188)
                     /locus_tag="VF874_01435"
     CDS             complement(1514..2188)
                     /locus_tag="VF874_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX9174794.1"
                     /translation="MWDSWGPVLAALIPLALVVALSPLSIIPAVVLVLHSPHPRRTGL
                     AFLLGWLAGLAGVTAIFVGVPRLLDGFHIQLPPWTDWVRIVLGVVLIVLAVWLWVKRK
                     RSTHQPKWLDRTKRITPTAAATIGIVLVLANPKVLAMNAAAGLVIGTGELGVPGVWLA
                     VAYYTALDGSTAAAPVLAHAVAAERVDRQLEHARRWMENQQAAITAVLLVLVGAVLVY
                     TGIQAA"
     gene            2297..4732
                     /gene="ponA2"
                     /locus_tag="VF874_01440"
     CDS             2297..4732
                     /gene="ponA2"
                     /locus_tag="VF874_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase/D,D-transpeptidase PonA2"
                     /protein_id="HEX9174795.1"
                     /translation="MSELPSTRVTVVKLLWCCLLAGVIAAGLMFPFAGGVGLLSNRAS
                     DVVANGSAQLIEGDVPQVSTMVDAAGNPIAWLYSQRRFEVRTDQIADTMKLAIVSIED
                     KRFIAHNGVDWQGTLTGLHGYLSGDTDTRGGSTIEQQYVKNYQLLVTAQTDAERRVAV
                     ETTPARKLREIRMALTLDKTFSKPEILTRYLNLVSFGNGAFGVQDAAETYFGIDASQL
                     DWQQAALLAGLVQSTTSLDPYTNPEGALQRRNLVLDTMIENFPAQAEALRAAKAEPLG
                     ILPQPQALPRGCIAAGNRAFFCDYVLDYLARAGISKDQVARGGYLIRTTLDPRVQDSV
                     KQAIDGIASPTLDGVANVMSVIKPGKDSHQVMAMASNRTYGLNMDAGETMQPQPFSLV
                     GDGAGSIFKIFTTAAALEMGMGINAQLEVPGRFEAKGMGSGGARGCPKETWCVQNDGG
                     YRGSMNVTEALATSPNTAFARLIQLVGVPRAVDMAVKLGLRSYAQPGTARAYDPESNE
                     SLADFVKRQNLGSFTLGPLELNPVELSNVAATLASGGVWCPPNPIAEIIDRGGNPVPM
                     TTESCEQVVPEGLANTLANAMSKDDQGNGTAAASAGSVGWDLPMSGKTGTTEAHRSSG
                     FVGFTNNYAGAVYIFDDSAEPTDLCSFPLRQCGSGNLYGGNEPARTWFEAMKPIATDF
                     GEVKLPPTDPRYVDGGPGSRVPSVSGLSVETAKQRLRDAGFGVADQTTPVNSTASYGT
                     VVGTAPSGQTVPGAIVTVNISNGIPPAPPPPPPGAPPLPFVVGSTVVEIPGLPPITVP
                     VLGPPPPPPPPPG"
     gene            complement(4680..6272)
                     /locus_tag="VF874_01445"
     CDS             complement(4680..6272)
                     /locus_tag="VF874_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9174796.1"
                     /translation="MGLRGDRIHRYVNAFCPRCHDERPDRPLSDVPRLSGWLAVRDER
                     VWLERGCPSHGLVRTLYDEDPEILAYLEEWTAPTKAHIPDVRGNFDPIPSAYERGLPE
                     MQTQHTCILLEDIAETCNLRCPTCFTDSSPDLHNVVAIGDVLANVDQRLERENGRIDV
                     LMLSGGEPTLHPQLPELLAELIARPITRILFNSNGIRIATDDALLDLLTQHRERVEVY
                     LQYDGLSLAAHRHHRGGDLRRVKATALQRLSEREIFTTLVMTAALGVNDGEIGDVVML
                     AMDTPFVGGVSIQPQFGSGRSGYLDPLDRLTHTGVLKRLGSQTNNLVTWHDLTALPCS
                     HPHCCSVGYLIRDDGGQWRSLVSLIGHDSLKEKLGLVANRIADSELPGELRLAVQESL
                     LGLLSEQSSLSHPQVGHLWRSICENCDLGMPTLLTLASAAALPGRRRKLRRLLGERVV
                     RVTIKPFMDMSTMLEERLVQCCVHVGTRSTQDQCAPFCAVQAWPQLGRQRLSASAKHL
                     LPIAAVSPGAAEVAAAARAPAP"
     gene            complement(6281..6676)
                     /locus_tag="VF874_01450"
     CDS             complement(6281..6676)
                     /locus_tag="VF874_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174797.1"
                     /translation="MTTPPPEPPEGGWPPPGYPYYPPHPPPRPPSSAGLIVGLAFVGA
                     FTYFVTNFVVAFVVLMLAADSSTSATVIATGTIGLALFAFGGGGVLLALRKPWAKGLG
                     LGLMIGWALTSLVTVGICTGINPSIYQGI"
     gene            complement(6673..7461)
                     /locus_tag="VF874_01455"
     CDS             complement(6673..7461)
                     /locus_tag="VF874_01455"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX9174798.1"
                     /translation="MVPQWRVGPAVLPVHGVFVALGVLAATVVFVAEARRRGAMNEQS
                     LVAVTGALVGGAIGMRLSGWAEHLDPRLNPTLMQVWEFGSRSILGGLLGAYLGVHIAK
                     RIGGYRGKTGDLFAPAVALGMAIGRIGCHLTEAPGRPTSLPWGVHAPSATPNCPGCLT
                     GQAMHPSFIYEIAFQLAAFAVLLWLRPRITHPGELFVIYIAGYAVFRFVVEFVRANDT
                     VWLDLTRPQWFLLPSLVVVFLRVRYGYRRGYYASLVFRRQEALA"
     gene            7548..8504
                     /locus_tag="VF874_01460"
     CDS             7548..8504
                     /locus_tag="VF874_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9174799.1"
                     /translation="MATSPLQRTAAAVAGGSALAWLGYASIVERNAFVVREVTMPVLT
                     PGSMPLRVLHLSDIHMRPKQRHKQAWLRELARWEPDLVVNTGDNLAHPRAVPAVVQTL
                     GELLSVPGLFVFGSNDYFGPRLKNPMNYVIDPGHRTHGTPLPWQDLRAAFAERGWLDM
                     THTRRELDIAGVKIAAAGVDDPHIDRDRYDTIAGPANPAADLSLGLTHSPEPRVLDRF
                     AADGYQLVLAGHTHGGQVCLPFYGALVTNCGLDRARAKGTSRWGATMQLHVSAGIGTS
                     PFHPVRFCCRPEATLLTLVAAPTGGPETTAASQRSDPTAALR"
     gene            8501..9598
                     /locus_tag="VF874_01465"
     CDS             8501..9598
                     /locus_tag="VF874_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HEX9174800.1"
                     /translation="MTRSAARDRPRAWVDTAVRLIEADTRRSADTHLLRYPLPSAWSS
                     RVDVQLYLKDESTHITGSLKHRLARSLFLYGLCNGWINEHTTVVEASSGSTAVSEAYF
                     AQLLGLPFIAVMPASTSAAKVALIESQGGRCHFVEESGEVYAEAQRLARETGGHYLDQ
                     FTNAERATDWRGNNNIAESIFTQLSLEQHPIPEWVVVGAGTGGTSATIGRYIRYRRFA
                     TRLCVVDPENSAFFPSYESGCDDVTTGMSSRIEGIGRPRVEPSFLPDVVDRMISVADG
                     ASIAAARHASEVLGRRVGPSTGTNLWGAFCLLAEMVADGRSGSVVTLLADSGDRYAET
                     YFCDDWLAEHGLDPTSAASTLIAFERTGSLD"
     gene            9926..9999
                     /locus_tag="VF874_01470"
     tRNA            9926..9999
                     /locus_tag="VF874_01470"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9960..9962,aa:Pro,seq:cgg)
     gene            complement(10131..10340)
                     /locus_tag="VF874_01475"
     CDS             complement(10131..10340)
                     /locus_tag="VF874_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9174801.1"
                     /translation="MRGRDRWSNEHGGLRFHDLRHSYGTWLADDDIVPNKLAKVMGHE
                     DIATTMQLYVRRTEDPDGIRDLLDG"
     gene            10514..>10724
                     /locus_tag="VF874_01480"
     CDS             10514..>10724
                     /locus_tag="VF874_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174802.1"
                     /translation="MWRSLLADALQADEDHLFGLHVDVALPRPLLLQMPVSTDVVDVL
                     FWRNGPYHGEREVLTAAGAVTVRAPR"
BASE COUNT         1842 a         3618 c         3591 g         1673 t
ORIGIN      
        1 gatcgacctg acgttcgtgc ggggttcgtt ggacgacgac gcgctgggca ggttgaccga
       61 cgagctcgtc actgtgctgt tgcgagccga acgcgctccg gacaccctgt tcctgcgcga
      121 caacacgctg gtgtacccgc acgcgctcga accgtccgcg ctgtcggtgg gcggccgcgg
      181 ccgcggtgag cctcgattcc gggtcgagct gaccgtgttc gaaggcgcat tgtcgcaaga
      241 ccgcaaggaa cgcctggtct ccgacgtgca cgcggcggtc tgcagtgcgg ccggaatcga
      301 tccgcagggc tcgacggcct atcatgtgtg gacgctgatt cacgaggttc ccgaaggcaa
      361 ttgggccggc ggtggaaaga tcatctacca ccagaatgtc aaagggctgg tggccgacga
      421 cgagccctga ctaacgtttg caggatggaa ggcgtagccg aactggtgcc ggtgcttcct
      481 gcgcaccgct tcgacgagga agctctcgcg ggctatctgc gcgggctgcc ggggttcgac
      541 ggccacctgg agatttgcca gttccaaggc ggacaaagca acccgacgtt tcacctgcgc
      601 accgccggcg gcgaatacgt gctgcgtaag aagccaccgg gcaagctact gccccgcgca
      661 cacgaagtag agcgggagca ccgcgtcatg tcggcgctgg ccgactccga tgtgccggtg
      721 ccgcgcatgc ggctgctgtg tcaggacgaa tccgtcatcg gcacccagtt tttcgtcatg
      781 gattatgtac ccgggcgcat cttccacgac cccggccttc gggaccttgc cccatctgat
      841 cgtgcggcga tctacgaaga cctcgcacga gtgcttgcgg cgctgcaccg ggtggactgg
      901 cgtgcagcag gtctggacgg gttcggcaag cctgacggtt acatgcaacg ccaggtggcg
      961 ctgtggacga ggcagtggca ggccgcgcgg gtggaagaca acccgacgat ggaccggttg
     1021 gcacagtggc tgcccgagca cctgccatcc gacgacgagc cggcgtgcat cgcacacggc
     1081 gactaccgcc tgggcaacgt gctgatccat ccgaccgagc cgcggatagt tgccgtgctg
     1141 gactgggagc tcgcgaccat cgggcatccg ctggccgacc tcggctacac ctgcctgacc
     1201 taccatctgc cggcgcactt catcggcacg aggggcgtcg caggagaaga cctcaccggc
     1261 actggaatcc ccgacgagtc cgcattcgtc gccagctact gccggtatgc cggccgaaac
     1321 gagcctgcct cgctcgacgt gttcgtggtg ttctcgatgt tccgtctcgc atccatcacc
     1381 gccggggtgt ggcgtcgcgg gctcgacggc aatgctgccg attcccgggc tggcacgtcg
     1441 gagttccgcg accgctaccg ggcgctggcg cagatggcgt ggacgctggc gcaacaactt
     1501 tcggatgcgt gagctacgcc gcctgaatcc ccgtgtacac cagcacagca ccaaccaaca
     1561 ccagcaacac cgccgttatc gcggcctgct gattctccat ccatcgcctg gcgtgctcga
     1621 gttggcgatc gacccgctca gcggcgaccg cgtgagccaa taccggagcg gcagccgtcg
     1681 agccgtccag cgcggtatag tacgccaccg ccaaccacac acccggtaca ccgagctcgc
     1741 ctgtgccgat caccaatccc gctgccgcat tcatcgccag caccttggga ttcgccagaa
     1801 caaggacaat cccgatcgtg gcggccgcgg tcggtgtgat ccgtttcgtc ctgtccagcc
     1861 acttcggttg gtgagtcgac cgcttccgtt tcacccagag ccagacggcc aggacgatca
     1921 gtacgacgcc gagcacaatg cgtacccaat ccgtccaggg cggcagctgg atgtggaagc
     1981 catcgagcag acggggcacc cccacgaaaa tggctgtgac accggctaat ccggctagcc
     2041 agccgagcag gaacgcgagc cctgtgcggc gcggatgagg ggagtgcagc accagaacga
     2101 cggccgggat gatcgacagc ggggacagcg cgaccaccaa cgcgagcgga atgagcgcgg
     2161 ccagcaccgg accccagctg tcccacatgg gtagttagcg tacgaaccgt tccaaatacg
     2221 gggcgaatcc gtaacgtcga ggaagccgcg ccccatgcgg ccggatgcgg tcgggggccg
     2281 cggataatct gtgtacatgt cggagttgcc gtcgacgcgg gtgacggtcg tcaagttgct
     2341 gtggtgctgc ctgctggccg gcgtcatcgc ggccgggctg atgttcccgt tcgccggcgg
     2401 ggtcgggttg ctgtccaacc gcgcatccga cgtcgtcgcc aacggctccg ctcagctcat
     2461 cgagggcgac gttccgcagg tgtcgacgat ggtcgacgcg gcgggcaacc cgatcgcctg
     2521 gctgtacagc cagcgccggt tcgaagtacg caccgaccag atcgccgaca caatgaagct
     2581 ggcaatcgtc tcgatcgagg acaagcgctt cattgcccac aacggcgtgg actggcaggg
     2641 cacactgacc ggcctgcacg gctacctctc cggcgacacc gatacccgtg gcgggtcgac
     2701 gatcgagcag cagtacgtga agaactacca gctgctggtc accgcgcaga ccgacgccga
     2761 gcggcgcgtc gcggtggaga ccacgccggc gcgcaagctg cgcgagatcc gaatggcgct
     2821 cacgctggac aagacgttct ccaagcccga gatcctcacc cgttacctca acctggtgtc
     2881 gttcggcaac ggggcgttcg gggtgcagga cgcggcggag acctacttcg gcatcgacgc
     2941 ctcccagctc gactggcagc aggccgcgct gttggccggc ctggtgcagt cgaccacctc
     3001 cttggacccc tacaccaacc ctgaaggtgc actacaacgc cgaaacctgg tgttggacac
     3061 catgatcgag aacttcccgg cccaagcgga ggcgctgcgc gccgcgaagg cggagccgtt
     3121 gggaatcctg ccgcagcccc aggcgctgcc ccggggttgc atcgcggccg gaaaccgcgc
     3181 gttcttctgc gactacgtgc tggactacct ggcgcgggcc ggcatcagta aggaccaggt
     3241 cgcccgtggc ggttatctga tccgcaccac gctggacccg cgggtgcagg actcggtcaa
     3301 gcaggccatc gacggcattg ccagtccgac gctggacggc gtcgccaacg tgatgagcgt
     3361 catcaaaccg ggcaaagact cccaccaggt gatggcgatg gccagcaacc ggacctacgg
     3421 cttgaacatg gacgccggcg agacgatgca gccgcagccg ttctcgttgg tcggtgacgg
     3481 cgccgggtcg atcttcaaga tcttcaccac cgccgccgcg ctggagatgg gcatgggcat
     3541 caacgcccaa ctcgaggtgc ccgggcggtt cgaggccaaa ggcatgggct cgggcggcgc
     3601 gcgcggatgc ccgaaggaaa cctggtgcgt gcagaacgac ggcggctacc gcggttcgat
     3661 gaacgtcacc gaagcgctcg ccacgtcacc caacaccgcc ttcgcccggc tcatccaatt
     3721 ggtcggagtg ccccgcgcgg tcgacatggc cgtcaagctc ggcctgcggt cctacgccca
     3781 acctggcacc gcccgggcgt acgacccgga aagcaacgaa agcctcgcgg acttcgtcaa
     3841 acggcagaac ctcgggtcgt tcacgttggg cccgctcgaa ttgaatccgg tcgagctgtc
     3901 caacgtggcg gcgacgctgg cgtcgggcgg cgtgtggtgc ccgccgaatc cgatcgccga
     3961 aatcatcgac cgtggcggca acccggtacc gatgaccacc gaaagctgcg agcaggtggt
     4021 gccggagggg ctggcgaaca cgctcgcgaa cgcgatgagc aaggacgacc agggcaacgg
     4081 caccgccgct gcgtcggccg gatccgtcgg ctgggacctg ccgatgtccg gcaagaccgg
     4141 caccaccgag gcgcaccggt cctccgggtt cgtcggcttc accaacaact acgccggcgc
     4201 ggtctacatc ttcgacgact cggccgagcc caccgacttg tgctcgttcc cgctgcggca
     4261 gtgcgggtcc ggcaacctgt atggcggcaa cgagccggcg cgaacgtggt tcgaggcgat
     4321 gaagccgatc gccaccgact tcggcgaggt caagctgcca cccaccgacc cgcgatacgt
     4381 cgacggcgga ccgggctccc gggtgccgag tgtgtcgggg ctttccgtgg agaccgccaa
     4441 gcagcggctc agagacgccg ggttcggcgt cgccgaccag accacaccgg tcaacagcac
     4501 cgcctcctac ggcactgtcg ttggcaccgc gccgagcggt cagaccgtcc ccggcgcgat
     4561 cgtcaccgtc aacatcagca acggcattcc gccggcgccg ccaccaccgc cacccggcgc
     4621 gccgccacta ccgttcgtgg tcggctccac ggtcgtcgag atccccgggc tgccaccgat
     4681 cacggtgccg gtgctcgggc cgccgccgcc acctccgccg cccccgggct gacggccgcg
     4741 atcggcagca ggtgcttggc cgaggcagac agccgctgcc ggcccaactg cggccacgcc
     4801 tgcaccgcgc agaacggggc gcactgatcc tgggtgctgc gggttccgac gtgcacgcag
     4861 cactgcacca gccgctcctc gagcatcgtc gacatgtcca tgaatggctt gatcgtgacg
     4921 cgcacgacgc gctcgccgag cagccggcgc agcttgcggc gacgtccggg cagggcggcg
     4981 gcggacgcca gggtcagcag cgtgggcatg cccaggtcgc agttctcgca gatactgcgc
     5041 cacaggtgac cgacctgggg gtgcgacagc gaggactgct ccgacaacaa ccccagcagc
     5101 gattcctgca cggccagccg cagctcgccg ggcagctcgc tgtcggcaat ccggttggcg
     5161 acgagcccga gtttctcctt gagactgtcg tgcccgatca atgacaccag cgaacgccac
     5221 tgcccgccat cgtcgcggat cagatagccc accgagcagc agtgcgggtg cgaacacggc
     5281 agcgcggtca ggtcgtgcca ggtgaccagg ttgttcgtct ggcttcccag ccgcttcagc
     5341 acgcccgtgt gggtgagccg gtcgagcggg tcgaggtagc cggagcgccc ggagccgaac
     5401 tgcggctgga tggacacgcc gccgacgaac ggggtgtcca tcgcgagcat gacgacgtcg
     5461 ccgatctcac cgtcattcac acccagggcc gcggtcatga cgagcgtggt gaagatctcc
     5521 cgctccgaaa gccgttgcag tgcagtggcc ttcacgcggc gtaggtcacc tccgcgatgg
     5581 tgccggtggg cggccagcga cagcccgtcg tactgcaggt acacctcgac gcgttcgcgg
     5641 tgctgggtca gaaggtcgag cagtgcatca tctgtagcga tgcgaatgcc gttgctgttg
     5701 aacaggattc gcgtgatagg ccgcgcgatg agttcggcga gcagctcggg cagctgcggg
     5761 tgaagggtcg gttcgccacc gctgagcatc aacacgtcga tccgaccgtt ttcccgctcg
     5821 agccgctgat cgacgttggc gagcacgtcg ccgatcgcga cgacgttgtg caggtccggc
     5881 gacgagtccg tgaagcatgt cgggcagcgc aggttgcatg tctcggcgat gtcttcgagc
     5941 aagatgcagg tgtgctgggt ctgcatttcc ggcagtccgc gctcatacgc cgacgggatc
     6001 gggtcgaaat tgcctcgcac atcggggatg tgggccttgg tgggggctgt ccactcctcg
     6061 agatacgcca ggatttccgg gtcttcgtcg tacagagtgc ggaccaggcc gtgggacggg
     6121 cagccgcgct ccaaccacac ccgctcgtcg cgcacggcta gccatccgct gagccggggc
     6181 acatcgctca gcgggcgatc gggtcgctcg tcgtggcagc gcggacagaa tgcgttgaca
     6241 tagcggtgaa ttcggtcgcc tcgaaggccc atgcctgcca tcaaatcccc tggtagatcg
     6301 acggattgat acctgtgcag atgccgaccg tgacgagcga cgtgagcgcc catccgatca
     6361 tcaacccgag acccaatccc ttcgcccatg gtttgcgcag cgccagcagc acgccgccgc
     6421 cgccgaacgc gaacagcgcg agcccgattg tgccggtcgc gatcaccgtt gcggacgtcg
     6481 acgagtcggc ggccagcatc agcaccacga acgcgacgac gaaattggtg acgaagtagg
     6541 tgaacgcccc gacgaatgcg agtccgacaa tgaggccggc cgagctcggt ggtcgcgggg
     6601 gcgggtgagg cgggtagtac gggtagccgg gcgggggcca gccaccctcc ggcggttccg
     6661 gcggcggcgt cgtcatgcga gtgcctcctg tcgccgaaac acgagcgatg cgtagtatcc
     6721 gcgccgatag ccgtacctca cgcgtaagaa cacgacgacc agcgacggca gcagaaacca
     6781 ttgcggccga gtcaaatcca accaaaccgt gtcgttggcc cgaacgaact cgacgacgaa
     6841 ccggaacacc gcatagcccg ctatgtagat gacgaaaagc tctcccgggt gggtgattcg
     6901 cggacgcagc cacagcagaa ccgcgaaagc cgctaattga aacgcgattt cgtagatgaa
     6961 cgacggatgc atcgcctggc cggtgaggca gccgggacag ttcggcgtgg cgctgggcgc
     7021 gtgcacgccc cacggcaggc tggtgggccg cccgggcgct tcggtgaggt ggcagccgat
     7081 ccggccgatc gccatgccga gcgccaccgc cggggcgaac aggtcgcccg tcttgccgcg
     7141 gtagccgccg atccgcttgg cgatatgcac acctaggtag gcgccgagca ggccgcccag
     7201 gatgctgcgt gacccgaact cccacacctg catcagcgtg gggttgagcc gcggatcgag
     7261 gtgttcggcc cagcccgaca gacgcatgcc gatcgcgccg ccgaccagcg cgcccgtgac
     7321 cgccacgagc gactgctcgt tcatcgcgcc gcgccggcgt gcctcggcga cgaagacgac
     7381 tgtcgcggcg agcacaccga gcgcgacgaa cacgccgtga acaggcagga ccgcggggcc
     7441 aacccgccac tgcggcacca ttcgcccagc ctattcggcg ccgaagcccc cggtttccag
     7501 aaaccgtcaa gcaggctccg tccaggactg cgcgataccc tgcctctatg gccacttcgc
     7561 ccctgcagag aacagcggcc gccgtcgcag gcggctcggc gcttgcctgg ctcggctacg
     7621 cctcgatcgt cgaacgcaac gcgttcgtgg tccgtgaggt gacgatgccg gtgctgacgc
     7681 cgggttccat gcctctgcgg gtgctgcacc tcagcgacat ccacatgcgc cccaagcagc
     7741 gccacaagca ggcgtggctg cgtgagctcg cgcgctggga gccggacttg gtggtcaaca
     7801 ccggcgacaa cctggcccat ccgcgggcgg tgccggcggt ggtgcagacg ctgggggagc
     7861 tgctcagcgt gcccggcctg ttcgtgttcg gcagcaacga ctacttcggc ccgcggctga
     7921 agaacccgat gaactatgtg atcgacccag ggcaccgcac ccacggcacg ccgctgccgt
     7981 ggcaggacct gcgcgctgcg ttcgccgaac gcgggtggct ggacatgacc cacacccgtc
     8041 gggaactgga catcgcggga gtgaagatcg ccgccgccgg tgtcgacgac ccacacatcg
     8101 accgcgaccg ctacgacacc atcgcgggcc cggccaaccc ggccgccgat ctgagcctcg
     8161 ggctgacgca ctcgccggag ccgcgggtgc tggaccggtt cgccgccgac ggctaccagc
     8221 tggtgttggc cgggcacacc cacggcggcc aggtgtgcct gccgttctat ggcgcgctgg
     8281 tcaccaactg cggcctcgat cgcgccaggg ccaaaggcac atcgaggtgg ggtgcgacca
     8341 tgcagttgca cgtgtcggcg ggcatcggca cgtcgccgtt ccatccggtg cggttctgct
     8401 gccgccccga agccacgctg ctgacgttag tggccgcgcc caccggcggt ccggagacaa
     8461 ccgcggcgtc acagcgctcg gatccgaccg ccgcgctgcg gtgacccgat cggcggcccg
     8521 tgaccggccg cgggcgtggg tggacaccgc ggtgcggctc atcgaggccg acacgcggcg
     8581 cagcgccgac acgcacctgc ttcgctaccc gctgccgtcg gcgtggtcgt cgcgcgtgga
     8641 cgtgcagctc tacctcaagg acgagagcac ccacatcacg ggcagcctga agcaccggct
     8701 ggcgcggtcg ttgttcctgt atgggctgtg caacgggtgg atcaacgagc acaccacggt
     8761 cgtcgaggcg tcgtcgggct cgacggcggt gtccgaggcg tacttcgcgc agttgttggg
     8821 cctgccgttc atcgcggtga tgccggcgtc gacgagcgcg gccaaggtcg cgctgatcga
     8881 gagccagggc ggccgctgcc atttcgtcga ggagtcgggc gaggtgtacg ccgaggccca
     8941 gcggctggcg cgggaaaccg gcgggcatta cctcgaccag ttcaccaacg ccgagcgcgc
     9001 gaccgactgg cggggtaaca acaacatcgc cgagtcgatc ttcacgcagc tgtcgctgga
     9061 gcagcatccg attccggagt gggtcgtcgt cggggcggga acgggcggga ccagcgcgac
     9121 gatcggccgc tacatccgct accggcgctt cgccacccgg ctgtgtgtgg tcgaccccga
     9181 gaattcggcg ttcttcccgt cctacgaaag cggctgcgac gacgtgacga ccgggatgtc
     9241 gtcgcgcatc gaagggatcg gccggccgcg ggtggagccg tcgtttctgc cggacgtggt
     9301 ggaccggatg atttcggtgg ccgacggtgc gtcgattgcc gccgcccggc acgcgagcga
     9361 ggtactgggc cgacgagtgg gcccgtccac cgggacgaac ctgtgggggg cgttctgcct
     9421 gctggccgag atggtggccg acggacggag cggctcggtg gtcacgctgc tcgccgacag
     9481 cggtgaccgc tacgcggaga cctacttctg cgacgactgg ctcgccgagc acgggctcga
     9541 tccgaccagc gctgcttcca cgctgatcgc gttcgagcgc accggctcgc tcgactgacc
     9601 gccctgcggt gacgcctccg cgcggtcggc cgtccagaac agccacagcg ccgccaaacc
     9661 gaagaaacct ccgtaaatcg cggctcccaa gcccgtcatg atcagccact ccttcgtcgc
     9721 tcgtgcttag tgtgcacaac ccggccgaca atccgggtca tcccgtgcgg cgtggtgagc
     9781 tgaatctcgg cgggcgtgcg cccaacgtgc gcccactgcg acgaatccac cccggaaatt
     9841 tcgcagtcac cgcacgctcg gcggaaggca gaacaacgcg tttggtcagc ggttggcccg
     9901 gtgcgatact ctggcgtggc ttcaacgggg tgtggcgcag cttggtagcg cgcttcgttc
     9961 gggacgaaga ggtcgtgggt tcgaatcccg ccaccccgac tggttggtcg agatgacaga
    10021 accgcccctg acctgcatcg aaggtcaggg gcggcgttgc ttctctgctt gatcactgcg
    10081 ctctccggtc caattaggca gcggagaggc agcattccga taaatcggcg tcacccgtcg
    10141 agcagatcac ggatcccgtc ggggtcctcg gttcgccgaa cgtacaactg cattgttgtg
    10201 gcgatgtctt cgtgccccat gaccttcgct agcttgttcg gaacgatgtc gtcgtcggcc
    10261 agccacgtcc cgtacgagtg tctgagatcg tggaacctca gccctccgtg ctcgttcgac
    10321 caccgatccc gacctctcac gtcctgcacc cgcgcagcgg cttcgggatg cccggcgtgg
    10381 gtggggctgg agtcaaaacg acctggccgc cgaagtcgag aaagtgcgga ccgtccgcaa
    10441 ccatcggccc tgcgaccgag agtcgttgcg ccggcagatc gtccaaatcg agagggacgg
    10501 caaagcgggt ccaatgtggc gatcactgct tgccgatgcg ttacaagccg acgaagatca
    10561 cctgttcggc ctgcatgtcg atgtcgcgct gcctcgcccg ctgctactgc aaatgccagt
    10621 cagcaccgac gtcgtcgacg ttctcttttg gcgcaacggg ccgtatcacg gcgaacgcga
    10681 ggtgttgacc gcagcgggtg cggtgacggt tcgggcgccg cgcg
//
LOCUS       DASWBF010000052         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_662592,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000052 DASWBF010000000
VERSION     DASWBF010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662592"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /gene="mqo"
                     /locus_tag="VF874_01485"
     CDS             complement(<1..1419)
                     /gene="mqo"
                     /locus_tag="VF874_01485"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEX9174803.1"
                     /translation="MDATATPKTDVVLVGAGIMSATLGALLRLVESDWSITLVERLDG
                     AAAESSGSWNNAGTGHSALCELNYTPERPDGSIDTTRGVRINEQFQVTRQFWAYGAEN
                     GVLTDVRSFINPIPHVSFAHGADQMDYLRRRRDALAGNPLFESIDYIEGSDEFARRLP
                     LMAEGRDLSEPIALEWSQDGTDVDFGSLTKQLIGYAVQNGTVALFGREVSGLRRESDG
                     TWTVTIRNNRTGEKRKINAKFVFVGAGGYALPLLQKSGIAEAKGFGGFPIGGKFLRSP
                     NPALVNAHQAKVYGFCAAGAPGNSAPHLDLRIISGKPWLMFGPFVGWTPKYLKQGRLT
                     DLFGSVKVGNLMSILNVGVTEMALVNYLIRQLRLSDGDLVEALREFAPAARDSDWETI
                     NAGQRVQIIRPKGRGGVLEFGTTTLAAADGTIAGLLGASPGASTAVPAMLDVLERCFP
                     DRYQLWLPKLKEMVPSLGTKLSE"
     gene            1490..2512
                     /locus_tag="VF874_01490"
     CDS             1490..2512
                     /locus_tag="VF874_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9174804.1"
                     /translation="MISVTATWEPDVLDGCWHTTLPLGSDPDGEGEIVATLIRRGEPS
                     RAKHVVLAVHGYTDYFFHTELADRLTARGFGFYAIDLRKCGRSWRNGQTPHFTTDLSR
                     YDAELEQAASLIGRETAGARILVYGHSAGGLIVTLWLDRLRRRGGGFGVAGLVLNSPF
                     FDLHGPAVLRTGVTSAAIRAANRFGSKRVLRATGEGGYGTSLHRDYGGEFDYNLTWKP
                     LGGFPVTVGWINAVRRAQMQLHHGLDVGVPNLILRSDKSIPETTDPATMQRGDAVLDV
                     AQIARWAGCVGNRTTVVPVADAKHDVFLSLPDAREVAYRELAAWLNWYTTFVESPQST
                     DARQLS"
     gene            2509..>3530
                     /locus_tag="VF874_01495"
     CDS             2509..>3530
                     /locus_tag="VF874_01495"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HEX9174805.1"
                     /translation="MTHFDLAIIGTGSGNTILDERYTDMNVAICEQNIFGGTCLNVGC
                     IPTKMFVYAAEIAESANDSERFGVNARVDGVRWPDIVSRIFGRIDPLSLGGENYRRSS
                     PNVTVFDSHTRFGPSRDGRHTLRVDGGEEFTADKVVIAAGSRAVVPKFIADCGISYYT
                     SDTIMRICELPKHLVIVGGGYVATEFAHIFSSLGSHVTLVIRGSTLLRGHDDTIMMRF
                     TDLAAKKWEIHAHRQVVDARQGGDEVEVICDDGSTVRGDALLVATGRAPNGDLLDAEL
                     AGVDVDSHGRVLVDEYQRTTARGIFALGDVSSAYQLKHVANHEARVVRHNLLCDWDDT
                     DSLVAS"
BASE COUNT          611 a         1172 c         1153 g          594 t
ORIGIN      
        1 ctcggacagc ttcgtgccga gcgacggcac catctccttg agcttcggca gccacaactg
       61 gtagcggtcg ggaaagcacc gctcgagtac gtcgagcatg gccggaaccg ccgtcgaggc
      121 gcccggagac gcaccgagca gaccggcgat ggtgccgtcc gcggcggcta gcgtggtggt
      181 accgaattcc agcaccccgc ccctgccctt gggccggata atctgcaccc gctgcccagc
      241 attgatcgtc tcccagtcgg aatcccttgc agccggagcg aactcgcgca gcgcctccac
      301 caggtcgcca tccgacagcc tcaactggcg aatcaggtag ttcaccagag ccatctcagt
      361 cacgccgacg ttgagtatcg acatcagatt gcccaccttg accgaaccga acaagtcagt
      421 cagacggcct tgcttcaggt acttcggtgt ccatcccacg aacgggccga acatcagcca
      481 tggcttgccg ctgatgatcc gcaggtctag gtgcggcgcc gagttgccgg gcgcccctgc
      541 cgcgcagaac ccgtagacct tggcctggtg ggcattgacc agcgccggat tcggcgaacg
      601 caggaacttg ccgccgatcg ggaagccgcc gaagcccttt gcctcggcaa tgccggactt
      661 ctgcagcagt ggcagcgcgt atccgcctgc gccgacgaac acgaacttgg cgttgatctt
      721 ccgtttctcg ccagtgcggt tgttgcggat cgtcaccgtc catgtgccgt cggactcgcg
      781 gcgcagaccg ctgacctcgc ggccgaacag cgcgactgtg ccgttctgca cggcgtagcc
      841 gataagctgc ttggtcagcg acccgaagtc gacgtcggtg ccgtcctggg accattcaag
      901 cgcgatgggt tcggacaggt ctcgcccctc agccattagc ggcagtcggc gggcgaattc
      961 gtctgagccc tcgatgtaat caatggattc gaacagtggg ttgcccgcga gcgcatcgcg
     1021 gcggcggcgc aggtagtcca tctggtcggc accatgagca aagctgacgt gcgggatggg
     1081 gttgatgaag ctgcgcacgt cggtgagcac cccgttttcg gcgccgtatg cccagaactg
     1141 ccgggtgacc tggaactgct cgttgatcct gacgccccgg gtggtgtcga tcgagccgtc
     1201 gggccgctcc ggggtgtagt tgagctcgca cagcgccgag tggccggtgc cggcgttgtt
     1261 ccacgaaccg ctgctttcgg cggccgcgcc gtcgagccgc tccaccagcg tgatcgacca
     1321 gtccgattcg acaagccgca gcagcgcgcc cagcgtggcg ctcatgattc cggctcccac
     1381 cagcacgacg tcggtcttgg gggttgccgt tgcgtctatc accactgccc aaggttatcc
     1441 actcgatgcg acggcgtcgt accaggcagc aggccacctg gcgattaagg tgatttctgt
     1501 gacggcgacg tgggagcccg acgtcctcga cgggtgttgg cataccacgc tgccgctcgg
     1561 ctctgatccc gacggcgagg gcgagatcgt cgccacactg atccgccgag gcgagccgtc
     1621 acgcgccaag cacgtcgtgc tggcggtgca cggctacacc gactacttct tccacaccga
     1681 actggcggat cggctgaccg cccgcgggtt cgggttctac gcgatcgacc tgcgcaagtg
     1741 tggacggtcc tggcgcaacg ggcagacccc gcacttcacg acggacctgt cgcgctacga
     1801 cgccgaatta gagcaggcag cgtcgctgat cggccgtgag acagccgggg cacggatcct
     1861 cgtctacggc cattccgcgg gtgggctgat cgtcacgctg tggctggacc ggcttcgccg
     1921 tcgcggcggc ggattcggcg tcgccggact ggtgctcaac agcccgttct tcgacctgca
     1981 cggcccggcg gtcctgcgca ccggcgtcac gtcggccgcg atccgggcgg cgaatcggtt
     2041 cggcagcaag agggtgctgc gcgcgacggg cgagggaggg tacggcacca gcctgcaccg
     2101 cgactatggc ggcgaattcg actacaacct gacgtggaag ccactcggcg gattcccggt
     2161 cactgtcggc tggatcaacg cggtccggcg cgcacagatg cagctgcatc acgggctcga
     2221 cgtcggcgta ccgaacctga tcctgcgctc ggacaagagc attcccgaga ccaccgaccc
     2281 ggccaccatg caacgcggcg acgcggtgct ggacgtggcg cagatcgccc gatgggcggg
     2341 ctgcgtcggc aaccgcacga ccgtggtgcc ggtcgccgac gcgaagcacg acgtgttctt
     2401 gtcgctgccc gacgcgcgtg aggtggcgta ccgggaactg gccgcatggc tgaactggta
     2461 caccacattc gtggagtcgc cacaaagcac cgatgcgagg cagctgtcat gacgcatttc
     2521 gatctggcca tcatcggaac cggttcgggg aacacgattc tcgacgagcg ctacaccgac
     2581 atgaacgtgg cgatctgcga gcagaacatc ttcggcggca cctgcctgaa tgtcggctgc
     2641 atcccgacaa agatgttcgt ctacgccgcg gagattgccg aaagcgccaa cgactcagag
     2701 cgattcgggg tcaacgcgcg cgtggacggg gtgcggtggc ccgacatcgt gtcgcggatc
     2761 ttcggccgca tcgacccgct gtcgcttggt ggcgagaact accggcgctc ttcacccaac
     2821 gtgacggtgt tcgacagcca cacccggttc ggccccagcc gcgacggacg ccacaccctg
     2881 cgagtcgacg gcggcgaaga gttcaccgcc gacaaggtgg tgatcgccgc cggctctcgt
     2941 gccgtggtgc cgaagttcat tgcggactgc ggtatttcgt attacaccag cgacacgatc
     3001 atgcggatat gcgaactgcc caagcatctg gtgatcgtcg gcggcggata cgtcgcaacc
     3061 gagttcgcgc acatcttctc gtcgctgggc agccacgtca cgctggtcat tcgcggcagc
     3121 acgctgctgc gcgggcacga cgacacgatc atgatgcgtt tcaccgatct cgccgcgaag
     3181 aaatgggaga tccacgccca ccgccaggtc gtcgacgccc gacagggtgg cgacgaggta
     3241 gaagtgatct gcgacgacgg ctccacggtg cgaggcgacg cgctgctggt cgcgaccggc
     3301 cgtgcaccca acggtgacct gctggacgcc gagctggccg gggttgacgt ggacagccac
     3361 ggccgggtgc tcgtcgacga ataccaacgc accactgcgc gaggcatttt cgcgctgggc
     3421 gacgtcagct cggcctacca actcaagcac gtcgccaacc acgaggcgcg ggtggtgcgg
     3481 cacaacctgc tgtgcgactg ggacgacacc gactcgctgg tcgcctccga
//
LOCUS       DASWBF010000053        10416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1189098,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000053 DASWBF010000000
VERSION     DASWBF010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189098"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VF874_01500"
     CDS             complement(<1..236)
                     /locus_tag="VF874_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174806.1"
                     /translation="MDTVVWIVIAIVVAIVLIAAVVLIARQSRTKKRRVEAERIREQV
                     REETVKVERREALADETAARARAAQAEAEAKAAEA"
     gene            complement(308..982)
                     /locus_tag="VF874_01505"
     CDS             complement(308..982)
                     /locus_tag="VF874_01505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9174807.1"
                     /translation="MSASRSPAVLFDIDGTLVDSNYLHVHAWARAFHDVGIAAEVWRI
                     HRSIGMDGSALVKTLSGDADDEVKEQLKDLHSRYYKELAPMLKVLPGGREILEQVPSM
                     GLQVVLATSAPDDELSILRDVLDRDNLVSEMTSSADVDTAKPQPDIIEVALKRAGVTA
                     NRAVFIGDSVWDAEACRRAGVPSIGVLSGGVSRDELTAGGAQVVFEDARELVEHVDDT
                     PIAEIR"
     gene            complement(1003..1677)
                     /locus_tag="VF874_01510"
     CDS             complement(1003..1677)
                     /locus_tag="VF874_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEX9174808.1"
                     /translation="MIAVQTSTTLRFAMLLTAANGFLDAYTFITRNGVFANVQTGNVI
                     FFAIDLSGGHWRNTLAHLWPILAFLVGVLLSAHIQSGRAEKLVSHPLRWTMAFQAVAF
                     AIVGFVPTTVPNSFVTVPISFLAAMQFTLFRNIGDLTYIAVATTGNLMRFVEAAYSVL
                     VDKDDAARLAFRVYGLLIATFSGGAVIGALASLCWGVRAAWIPAALLAVTLVFFIVDE
                     RKGKEP"
     gene            complement(1677..2801)
                     /locus_tag="VF874_01515"
     CDS             complement(1677..2801)
                     /locus_tag="VF874_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9174809.1"
                     /translation="MSGPADASALAEHLGHLRLVDHHVHGCWVDKGDRRRFENALNEA
                     NTDPLAEFDSGFDTQLGFAVRAHCAPLLGLPPHAAPDDYWRRRCEYTEDELARLCLRG
                     AGVSDWLVDSGLPDGTADVDGMAAFSAGRAHEVIRLEQIAEQAVTDPGDYADAFDHHL
                     NRRAAAAVATKSILAYRGGFDGDLSEPTRTEVADAAVRWRDHGGRLNDRVLLRFGLHR
                     ALRLGKPLQLHVGLGDRDADLHKTNPLLLLDFLRASGDTPIMLLHCYPYEREAGYLAQ
                     AFNNVYVDGGLSINHLGARGGGVVGRLLELAPFRKILYSSDGFGPAELHYLGAILWRN
                     GIRRVLQCIVDAGEWSEADAIWVTDLIGYRNAVRVYGIDA"
     gene            complement(2798..4147)
                     /locus_tag="VF874_01520"
     CDS             complement(2798..4147)
                     /locus_tag="VF874_01520"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX9174810.1"
                     /translation="MTDEAPAAPSIAQLEAGIETVIGTVVNPAGLTHAKMVPPRRLSA
                     FADPGLGASPVWHVFAIDQTGIAFTKEIGVVGDERIRLDLTALRTLGDGLAWAPGSYF
                     DQRGAPIPACSRGTLRRVADRMQRAGLEAQFGHEIEFLLVAPDGSRLPSALWAQYGLA
                     GVLEHEGFVRDVTTAAAAAGIGIEQFHPEYGQNQFEISLSPLAPVAAADQLVLMRLVI
                     GRVARRYGARVSYSPVPFAGSVGSGAHQHFSLTKDGAPVFSGGSGVEGMTEDGEHAIA
                     GILVGLPEAQGVLSGSIVSGFRMQPGFWAGANVCWGTENREAAVRFLVGGPANPYGAN
                     VEVKVVDPSANPYFASAVILGLALDGIEQRTPLPPEVTVDPASLGDDERSRAGVVTLP
                     TDQAAVIDALDGSSRIRDILGDAAVDATVAVRRYEHDTYGDLEPAELADKFRMAWSL"
     gene            4255..4749
                     /locus_tag="VF874_01525"
     CDS             4255..4749
                     /locus_tag="VF874_01525"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9174811.1"
                     /translation="MTTDHSAFDDLVGLLDYPMFVVTTRAGDDRAGCLVGFASQVSIR
                     PSRFLAGLSKKNHTFRVASRATHLAVHLLPREHRDMARLFGSETGDRVDKFSRCAWHD
                     GPEGLPILDDAVGWFAGRILNRFDVGDHVGFLPQPVAGHAPEHFGELVTFADMRDLEP
                     GHEA"
     gene            4902..5003
                     /locus_tag="VF874_01530"
                     /pseudo
     CDS             4902..5003
                     /locus_tag="VF874_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5082..5975)
                     /locus_tag="VF874_01535"
     CDS             complement(5082..5975)
                     /locus_tag="VF874_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9174812.1"
                     /translation="MSYQTILYATDGPIARVTLNRPDELNTIVAPMPDEVEDAVNTAA
                     RDPSVKVIVLRGAGRAFCAGYHFGGGFRYGADFLSTDGRWDPGKDMMHTTAAATSPHQ
                     KFMSIWRSPKPVIAQVHGWCVGGGSDYALCADLVIASEDAQIGTPYSRVWGAYLSGMW
                     IYRLGLTRAKYHALTGKPLSGREAADVGLINQAVPFADLEAVVRAQAEELASLPMGQL
                     AAQKLMVNQAYENMGLASTQTLGAILDGIMRNIPEAHAFIEHAAAQGVSAVVAERDGR
                     FGDYSQAPPAGKPNPDNVIEP"
     gene            6060..6347
                     /locus_tag="VF874_01540"
     CDS             6060..6347
                     /locus_tag="VF874_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174813.1"
                     /translation="MTRSPLLSRLVGRAFAIVTYTGRRSGKRVSLPVGYRRCDDELLI
                     GVAMPDAKTWWRNFLGEGGPVTVTLDGRERAGHAVATRDDGGVTVRVQLRP"
     gene            complement(6337..6819)
                     /locus_tag="VF874_01545"
     CDS             complement(6337..6819)
                     /locus_tag="VF874_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9174814.1"
                     /translation="MSRIGNFADDDLTSWFAASPELGGALANFSDAVYHCSRLPLRVR
                     EIARMAIALDNECAVCENTRFGEAPAAGVDEEFYAHVPEWRTWPGYSDEERLAAEFGQ
                     RFASDHTGLREDEAFWERWHELLSDEILADLALSCALGVGMGRVLRTLDIGQACKLTV
                     "
     gene            complement(6878..7996)
                     /locus_tag="VF874_01550"
     CDS             complement(6878..7996)
                     /locus_tag="VF874_01550"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006900509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX9174815.1"
                     /translation="MTRNTITLTPEQTEAFGRELDALKQRVVADLGERDASYIRRVIK
                     AQRAFKVGGRALLMAGILPPAWLAGTAMLAVSKILDNMEIGHNVMHGQYDWMGDPALS
                     SRGFEWDTACPGDQWRHSHNYMHHTYTNIVGMDRDVGYGILRMSEDQRWQPYYLGNPL
                     YAVLLMMLFQYGVAAHELETENIRAGKITWSDKRGVLEEMWRKTRRQLLKDYVAFPLL
                     AGPFAPVVAAGNLTANLTRNIWAFMIISCGHFPEDVQEFSIEETENETRGQWYFRQIL
                     GSANLTGGRLFHLLTGNLSFQIEHHLFPDIPAHRHAEISVEVRQFCERYGIPYNTGPL
                     HRQFGTVVRKIVRLALPPMQKFAELVPLRRRQDPAAAA"
     gene            complement(8480..9532)
                     /locus_tag="VF874_01555"
     CDS             complement(8480..9532)
                     /locus_tag="VF874_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9174816.1"
                     /translation="MTIVGGFDVHRKQITFDYVDTDTGLVRCGEIRPATRKTLRSWLG
                     EHCPDGDAEMAVEGCTGWRYVVEELAAAGANAHLADPAEVAGLRGPKKRAKTDRADAR
                     LLRTLLLQGRLPESWIPPTHMLEVRTLGRLYCALMDERRAWRQRIHAQLFHQGCPPIT
                     ALLSEAGREALARAELSATGRQYVDTALRRIDELTGEIDPVRMQLVNFARRQVGCQAL
                     QSRQYGVGWLCAAIMWAEIGDARRFHSSDQLVRFAGMDVTVYSSDGKRSPGHLSRQGC
                     PELRWATFEAAKCAARRGSPDYAYYQRLAARPDSGKNPTLAVERKILRRSYHTLRELG
                     DAALAMPVTKSQEVAA"
BASE COUNT         1725 a         3468 c         3475 g         1748 t
ORIGIN      
        1 gcttccgctg ccttggcctc ggcctccgcc tgggcggcgc gggcccgcgc ggcggtttcg
       61 tcggcgagtg cttcgcgccg ctcgaccttc acggtttcct cacgaacctg ctcgcggatg
      121 cgttcggctt cgacgcgacg cttcttggtg cggctctgcc gcgcgatgag aacgacagcg
      181 gcgatcagca cgatcgcgac cacgatcgcg atgactatcc agacgacggt atccatgacc
      241 gctcctaatt gacgtacgtg tcggtcacta cagcgtgccc gcgcccgacc tggttgaaac
      301 ctcgtcgtca gcggatttcg gcgatgggcg tgtcgtcgac gtgctcgacg agttcgcgcg
      361 cgtcctcgaa caccacctgg gcgccgccgg ccgtcaactc gtcacgggaa accccgccgc
      421 tgagcacgcc tatgctggga acgcccgcgc gccgacaggc ctccgcgtcc cagaccgagt
      481 cgccgatgaa gaccgcccgg ttggccgtta caccggcgcg cttcagtgct acctcgatga
      541 tgtcgggctg gggcttggcg gtgtcgacgt cggcggacga cgtcatctcc gacacgaggt
      601 tgtcccggtc cagcacgtca cgaaggattg acaactcgtc gtcgggcgcc gacgtcgcga
      661 gcaccacctg caggcccatc gacgggacct gctcgaggat ttcacgtccg cccggcaaca
      721 ccttcagcat cggcgccagc tccttgtagt agcgcgaatg caggtctttg agctgctcct
      781 tcacctcgtc gtcggcatct cccgacagcg tcttgaccaa tgccgagccg tccatcccga
      841 tcgatcggtg gatacgccat acctctgcgg caatcccgac atcgtggaag gcgcgtgccc
      901 acgcgtgcac gtgcaagtag ttggaatcga cgagggtgcc gtcaatgtcg aacaggacag
      961 caggggaacg tgaagcgctc attccgggtg gatacccccg cgctagggtt ctttaccttt
     1021 gcgttcgtcg acgatgaaga acaccagtgt caccgccaga agcgccgcgg gaatccaggc
     1081 tgcccgcacg ccccagcaca ggctggcgag cgcaccgatc acagcaccgc cggagaacgt
     1141 ggcgatcaat aggccgtaga cccggaacgc gagccgcgcc gcgtcgtctt tgtcgacgag
     1201 gacggagtag gcggcttcga cgaacctcat cagattcccg gttgtcgcca ccgcgatgta
     1261 ggtcaagtcg ccgatgttgc gaaacagcgt gaactgcatc gcggccagga acgaaatcgg
     1321 taccgtgaca aagctgttgg gcaccgtggt cggcacgaaa ccgacgatcg cgaatgcgac
     1381 ggcctggaag gccatcgtcc accgcaacgg atgcgacacc agtttttcgg cgcgcccgga
     1441 ttggatgtgc gccgaaagca gcacgcccac aaggaaggcg agaatcggcc acaggtgcgc
     1501 cagcgtgttt cgccaatgcc cgccggagag gtcgatggcg aagaagatga cgttgccggt
     1561 ctgcacgttg gcgaagacgc cgttgcgggt gatgaacgtg taggcgtcca ggaacccgtt
     1621 ggcggcggtg agcagcatcg cgaagcgcag cgtggtcgac gtctgcacgg cgatcatcag
     1681 gcgtcgattc cgtacacgcg gaccgcgttg cggtagccga tcaggtcggt gacccagatc
     1741 gcatcggcct cgctccactc ccccgcatcg acgatgcact gcagcacacg acgaattccg
     1801 ttgcgccaca agatggctcc taggtagtgc agctccgcgg ggccgaaccc gtccgacgag
     1861 tacaggatct tgcggaacgg ggccagctcg agcaggcggc cgacgacccc gcctccgcgt
     1921 gcccccagat ggttgatcga cagcccgccg tctacgtaga cattgttgaa agcctgtgcg
     1981 agataaccag cttcgcgttc gtacgggtaa cagtgcagca gcatgatcgg ggtgtcgccg
     2041 ctcgcacgca agaagtccag cagcagcagc gggttggtct tgtgcaagtc ggcgtcgcga
     2101 tcgcccagac cgacgtgcag ctgcaatggc ttgcccagcc gcagcgcacg gtgaagcccg
     2161 aaccgcaaca gcacccggtc gttcagacgg ccgccgtggt cacgccaccg tacggcggcg
     2221 tcggcgacct ccgtgcgcgt cggctccgac aggtcgccgt cgaagccgcc gcgatacgcc
     2281 aggatcgact tggtcgccac cgcggcggcc gcacgccggt tcaggtggtg gtcgaacgcg
     2341 tcggcgtagt caccggggtc ggtgacggct tgttcggcga tctgctccag cctgatcacc
     2401 tcgtgcgcgc gaccggcaga gaacgcggcc atgccgtcga catcggcggt gccgtccgga
     2461 agtccgctat ccacaagcca atccgacacg cccgccccgc gcaggcacaa ccgcgccagc
     2521 tcgtcctcgg tatattcgca gcgccgccgc cagtagtcgt cgggcgccgc gtgcgggggc
     2581 agtccgagca gcggcgcgca gtgcgcgcgc accgcgaaac cgagctgggt gtcgaagccg
     2641 gagtcgaact cggcgagggg gtcggtgttg gcctcgttga gcgcgttctc gaaacggcgc
     2701 cggtccccct tgtcaaccca gcagccgtgt acgtggtggt cgaccaggcg caaatggccg
     2761 aggtgttctg cgagggccga cgcgtcggcc ggcccgctca cagactccac gccatgcgga
     2821 acttgtcggc cagctccgcc ggctccagat ctccgtaggt gtcgtgctcg tagcggcgca
     2881 ccgcgaccgt ggcgtcgacg gcggcgtcgc cgaggatgtc gcggatccgg ctcgacccgt
     2941 ctagcgcgtc gatcaccgcg gcctggtcgg tgggcagcgt gaccacccct gcccggctcc
     3001 gctcgtcgtc gccgaggctg gccgggtcca cggtgacttc cggcggcagc ggggtgcgtt
     3061 gctcgatgcc gtcgagggcc agcccgagga tcaccgcgga tgcgaagtac gggttcgccg
     3121 aagggtcgac gaccttcacc tccacgttcg cgccgtacgg gttggccggt ccgccgacca
     3181 ggaaccggac cgccgcttcc ctgttctccg tgccccagca gacgttcgcc ccggcccaga
     3241 accccggctg catccggaaa ccggagacga tcgatccgct gagcacgccc tgcgcttcgg
     3301 gcagtccgac cagaattccg gcgatcgcgt gctcaccgtc ctcggtcatt ccctcgacac
     3361 ccgatccgcc ggaaaacacc ggcgcaccgt ctttggtcag cgaaaagtgt tggtgggcac
     3421 cggatcccac gctgcccgcg aacggcacgg gtgaatagct gacccgcgca ccgtagcgac
     3481 gggccacccg gccgatgacc aagcgcatca ggaccaactg gtcggccgcg gcgaccgggg
     3541 ccagcgggga cagtgagatc tcgaactggt tctggccgta ttccgggtgg aactgctcga
     3601 tgccgatacc ggccgccgct gctgccgtgg tgacgtcccg gacgaacccc tcgtgttcga
     3661 gcacgccggc cagtccgtac tgcgcccaca gcgcggacgg caaccggctg ccgtcgggtg
     3721 cgacgagcag gaactcgatt tcgtggccga actgcgcctc gaggccggcg cgctgcatcc
     3781 ggtcggcgac gcggcgcaat gtgccgcggc tgcacgccgg tatcggtgcg ccgcgctggt
     3841 cgaagtacga gcccggggcc caggccaacc cgtcgcccag cgttcgcagc gcggtcaggt
     3901 ccagccggat gcgctcgtcg ccgacgacgc cgatctcctt ggtgaacgcg atgccggtct
     3961 ggtcgatcgc gaagacatgc cagacggggc tggcgcccag gccggggtcg gcgaatgccg
     4021 acaatcggcg cggcggaacc attttggcgt gggtgaggcc agcgggattc accaccgtgc
     4081 cgatcaccgt ttcgattccc gcctcgagct gggcgatgga aggcgcggcc ggcgcttcgt
     4141 ctgtcatctc agatattgtg cagcgccgca ggtcctcgcg tgagcagacg cagaagcttg
     4201 cttgtcaccg cgcagtgaac tccgccatcg gtgttccgta cggtgcgaag tgcgatgacc
     4261 acagaccatt cggcgttcga cgacctcgtc gggctgctcg actacccgat gttcgtcgtc
     4321 accacccgag cgggtgacga ccgagccggg tgcctggtcg gattcgccag ccaggtcagc
     4381 attcgtccgt cacggtttct cgccggattg tccaagaaga accacacctt ccgggtcgca
     4441 agccgggcaa cgcacctggc cgttcacctg ctgccgcgcg aacaccgcga catggcgcgg
     4501 ctgttcggca gcgaaaccgg cgaccgggtg gacaagttca gccgatgcgc gtggcacgac
     4561 ggaccagaag gactgccgat cctggacgac gccgtcggct ggttcgccgg gcgcatcttg
     4621 aaccgcttcg atgtcggtga tcacgtcggc ttcctgccgc aaccggtcgc cggccacgcg
     4681 ccggagcact tcggagaact cgtcacgttc gctgacatgc gcgacctgga acccggacac
     4741 gaggcgtgag cacctcgggt ttgcccggca acagcggggg cactcattag cggtaaccgg
     4801 tcgacacagg agggcttcat gcatgccgaa cgcttccgac acctaactat ccgcggaagc
     4861 cccgttcgct tcgatctatt tcgacgactc ccacgacgat ggcggcgatt gcgaccgacg
     4921 cgaacctggt gcgcaccgac gaacggatcg cgccggccga cggcgtggcc gcggtgctgc
     4981 ggtacgcccg cactcaccac tgatgtgcgc gaaactgtat tttcacaggg aaagccgagg
     5041 agtgacggcc tgtggaatac agtttcggcg cgagcggtgc ctcagggttc gatgacgttg
     5101 tccgggttgg gcttgcccgc cggcggtgcc tggctgtagt cgccgaaccg gccgtcgcgc
     5161 tcagccacca ctgctgagac gccttgtgcg gcagcgtgtt cgatgaacgc gtgcgcttcg
     5221 gggatgttgc gcatgatgcc gtcgaggatc gcgccgagcg tttgggtgga cgccaggccc
     5281 atgttctcgt aggcctggtt gaccatcagt ttctgcgcgg ccagttgccc catcggtaaa
     5341 gaggcaagct cctcggcctg tgcgcgcacg accgcctcga ggtcggcgaa cggcaccgcc
     5401 tggttgatca gcccgacatc ggccgcctcc cggccggaca gcggcttgcc ggtcagtgcg
     5461 tggtacttcg cgcgggtcag gccgaggcgg taaatccaca ttccggacag gtacgctccc
     5521 cacacccgcg agtacggcgt gccgatctgc gcgtcctcgc tggcgatcac caggtcggcg
     5581 cacaacgcgt agtcgctgcc gccgccgacg caccagccgt gcacctgcgc gatgaccggc
     5641 ttcggcgagc gccagatgct catgaacttc tggtgcggcg acgtggccgc ggcggtggtg
     5701 tgcatcatgt ccttgcccgg gtcccaccgc ccgtcagtgc tgagaaagtc tgcgccgtaa
     5761 cgaaatccgc cgccgaagtg gtagccggcg caaaacgcgc gtcccgcccc gcgcagcacg
     5821 atgaccttca ccgacgggtc gcgcgccgcg gtgttgaccg cgtcttcgac ctcgtcgggc
     5881 atcggcgcga cgatcgtgtt cagctcgtcc ggcctgttca gcgtgacccg cgcgatcggg
     5941 ccgtcggtgg catacaggat cgtctggtag ctcatgggcg gctcctggaa gatggtgacg
     6001 tgcaacacga gcgactgata ctgcgtgctg cgcccatagt caacgcccca gtggcggcga
     6061 tgaccagatc gcctctactg agccggttag tcggccgtgc gttcgcgatc gtgacctaca
     6121 ccggtcgacg gtcagggaag cgcgtcagcc tgccggtcgg ctaccgccga tgcgacgacg
     6181 aactgctgat cggcgtcgcg atgcccgacg ccaagacgtg gtggcgcaat ttcctcggcg
     6241 agggcgggcc ggtgaccgtc accctcgacg ggcgtgagcg ggccggacat gcggtcgcca
     6301 cccgcgacga cggcggcgtc acggtccgcg ttcagctcag accgtgagct tgcaggcctg
     6361 gccgatgtcc agcgtgcgca gcacccggcc catgccgacc cccagcgcgc acgacaacgc
     6421 gaggtcggcg aggatttcgt cggacagcaa ttcgtgccag cgctcccaga aggcctcgtc
     6481 ctcgcgcagg ccggtgtgat cggaggcgaa gcgctggccg aactcggcgg cgagcctctc
     6541 ttcgtcgcta tagcccggcc acgtccgcca ctcgggcacg tgcgcgtaga actcctcgtc
     6601 gacgcctgcg gcgggcgctt cgccaaaccg ggtgttctcg cacaccgcgc actcgttgtc
     6661 cagcgcgatc gccatgcgcg cgatctcgcg gactcgcagt ggcaaccgac tgcagtggta
     6721 gacggcgtcg ctgaagttgg caagcgcacc gccgagttct ggcgaggcgg cgaaccaact
     6781 ggtcagatca tcgtcggcga agtttccgat ccggctcatg gcacgcgata gtacgccgtg
     6841 ccacgcgaat ctgtaacacg ttctagttcg gtgaccgtca ggccgccgcg gcggggtcct
     6901 gccgcctgcg caacggcacg agttcggcga acttctgcat gggcggcaac gccagccgca
     6961 cgatcttgcg gaccaccgtg ccgaactgcc ggtgcagcgg cccggtgtta tacgggatcc
     7021 cgtaccgctc gcagaactgc cgcacctcga cggagatttc cgcgtgccgg tgcgccggga
     7081 tgtcggggaa caggtggtgc tcgatctgga acgacaggtt tccggtcaag aggtgaaaaa
     7141 gcctgccgcc ggtcaggttc gccgacccga ggatctggcg gaagtaccac tggccgcgcg
     7201 tctcgttctc cgtctcctcg atcgagaact cctggacgtc ctcggggaag tggccgcaag
     7261 agatgatcat gaacgcccag atgttgcggg tcaggttcgc cgtcaggttg ccggccgcga
     7321 cgacgggcgc gaacggaccg gccagcagcg ggaacgcgac gtagtccttg agcagctggc
     7381 gacgcgtctt gcgccacatc tcttccagca cgccgcgttt gtccgaccag gtgatcttgc
     7441 cggcgcggat gttctcggtt tccaattcgt gtgccgcaac gccgtattgg aagagcatca
     7501 tcaacagaac cgcgtacagc gggttaccca ggtagtacgg ctgccaacgc tgatcctcgc
     7561 tcatccgcag gatcccgtag ccgacgtcgc ggtccatgcc gacgatgttg gtataggtgt
     7621 ggtgcatgta gttgtgcgag tggcgccact ggtcgccggg gcaggcggtg tcccactcga
     7681 agccgcggct ggacagagcc gggtcgccca tccagtcata ctggccgtgc atcacgttgt
     7741 ggccgatctc catgttgtcc aagatctttg acaccgcaag catggcggtg ccggcgagcc
     7801 aggccggcgg cagaattccg gccatcagca gcgcgcgccc gccgaccttg aatgcccgtt
     7861 gggccttgat gacgcggcgg atgtagctgg cgtcccgctc gcctaggtcg gcgacgacgc
     7921 gctgtttcag tgcgtcgagt tcgcggccga aggcctcggt ctgctcgggg gtcagggtga
     7981 tcgtgttcct ggtcatggct ggttccttat ctgtgttggg gacccccgta aaggtgagag
     8041 tccgttggtt tccttggttg tttggccttg ttccccgtcc cgtgccaggg cggtggaccg
     8101 cagtcggggt cgaggtggag cgcccgcagc gcagcgagga cgaacgacct tgaggccggc
     8161 gaggatccgc cgcatactcg gtcgggcgaa gggggacgag gcaaaggcgt ggtggttcaa
     8221 gtcatgtttg cctcccgtgc gagaacatgg tgcgcgggga gacgtcgtgg ggcgtgccgg
     8281 cgcccagcct tatctgggtc cacggacgag ggtccggcga catgatgatg gatggggtgc
     8341 ccggcgtgcg ggctggtgat gggtgcggcc gctcattctt tccggggcgt ccacccaggc
     8401 agcgggtggc ggcaggggga ttgcgggagc tggccgcggg catcagctga tgtatgggca
     8461 gggcacgcgc acaacgcatt taggcggcca cctcctgcga tttggtgacc ggcatcgcca
     8521 gggcggcatc gccgagctca cgcagcgtgt gatagctgcg gcgcaggatt ttgcgttcga
     8581 ccgccagggt cgggttcttg ccgctgtcgg ggcgggcggc cagccgctgg tagtaggcgt
     8641 agtcggggga accgcggcgg gccgcgcact tagccgcctc gaaggtggcc caccgcagct
     8701 cggggcagcc ctgccgggac agatgccccg gtgagcgctt gccgtctgag gagtacacgg
     8761 tgacatccat cccggcgaag cgcaccagct gatcggagct gtggaaccgg cgggcatcgc
     8821 cgatctcggc ccacataatc gccgcgcaca accaacccac cccgtactgg cgggactgca
     8881 gcgcctgaca ccccacctgg cgccgggcga agttcaccaa ttgcattcgt accgggtcga
     8941 tttcgccggt cagctcatca atgcggcgca gcgccgtgtc cacatactgt cggcccgtcg
     9001 ccgacagctc ggcacgcgcg agcgcctcac ggccggcctc ggacagcagc gcggtaatcg
     9061 gcgggcagcc ctggtggaac aactgggcat ggatgcgctg ccgccacgcg cgccgctcat
     9121 ccatcagcgc gcaatacaac cggcccaggg tgcgcacctc cagcatgtgg gtgggcggga
     9181 tccacgattc gggtaaccgc ccctgcaaca gcagcgtgcg caacaaccgc gcgtcggccc
     9241 ggtcggtctt ggcccgcttc ttcggacccc gcaaccctgc gacctcggcc ggatcggcca
     9301 gatgcgcgtt ggctcccgca gcggccagct cctcgaccac atagcgccac ccggtgcatc
     9361 cctccaccgc catctccgcg tccccatccg ggcagtgctc gcccagccag ctccgcagcg
     9421 tcttgcgggt cgccggacgg atctcaccgc agcgcaccaa cccggtatcg gtgtccacgt
     9481 aatcgaacgt gatctgcttt cgatgcacgt caaacccgcc tacgatcgtc attgggacct
     9541 cctcaagctc ccgaagaaac tctgacgcaa actcaacgag cgtctgagct gagggggtcc
     9601 ccatcacatg tcatctttcc ttgtgcgcgt tggcctttca gctacctgtc agacaagccg
     9661 cccagaccgc ggtcggtgcc tgaatgaatg aatcatcggt cagcccaccg gacggtcggc
     9721 aggtcacgga cgaacttgga tgctcgcgac ggtcttgtcc aacttgtgta actcaacgtc
     9781 gacgctgcca ggcacatcga cagtgaactg aaaggtctgg tttgatgccg ccgcaatctc
     9841 gaactcgtga tcgggtgtcg agtgcacgtg caattcgtcg ggggcgtcag cccgcgggca
     9901 cgggccggtc gggacgcccg gtgcgggggt agacgtagag gacggcacgg ccgctgccca
     9961 gctccgccag atcgaccggg ccctgcgacg agtcgagcgt cagcgacggt accgggagac
    10021 cggtcaggtg gtcgcaggcg ccgtcgtcga ccggcgctgg cagatcgggc ggaagcccct
    10081 agacgctcac ccggccctac agagttgtgc agcctcaaca cagagcatcc ccgcctcggc
    10141 atctttaatg aaccccatgc gcggatcgta gcgacgcgac accggcgaga tgggcgagcc
    10201 tcgctggcca ggcttgggca gttgtgcgcg gggtcccacg tgaatttcga agcgggacct
    10261 gttctcgtat ccgtagacca ggtggcgtga tgcatcaagt caagatgccc gcgaaatctg
    10321 atccatgcat cacgtaagga atgcccgcga aatcggggtc agctggcggc ttgacgacct
    10381 cgcgggtggc gatgatgatg atgtagtgca gcccgc
//
LOCUS       DASWBF010000054         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1121024,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000054 DASWBF010000000
VERSION     DASWBF010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121024"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VF874_01560"
     CDS             complement(<1..694)
                     /locus_tag="VF874_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9174817.1"
                     /translation="MKWQDRADVVVIGTGVAGLAAALAAHRRGSRVIVLSKTADTATF
                     YAQGGIAVVLPRTSDSIDAHVNDTVAAGGGLCDPDAVRSIVADGYRAVSELVSDGARF
                     DETAPGRWALTREGGHSRRRIIHAGGDATGAEVQRALDHAAAILDIRRNHVARQLLYD
                     NDGITGVEVISDDGVGIVHAPAVILATGGLGNLYRATTNPEGSTGDGVALALWAGVPV
                     SDIEFVQFHPTML"
     gene            complement(691..1692)
                     /gene="nadA"
                     /locus_tag="VF874_01565"
     CDS             complement(691..1692)
                     /gene="nadA"
                     /locus_tag="VF874_01565"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX9174818.1"
                     /translation="MTVLDHGVYTGVEPDAEWAAEVRRLAELRNATLLAHNYQIPAIQ
                     DVADHVGDSLALSRIAAEAAEQTIVFCGVHFMAETAKILSPEKTVLIPDQRAGCSLAD
                     SITADDLAAWKAEHPGAVVVSYVNTTAAVKALTDICCTSANAVDVVASIPEDREVLFC
                     PDQFLGAHVRRVTGRKNLHVWAGECHVHAGINGDELADQARSHPDAELYVHPECGCAT
                     SALYLAGEGAVPAERVKILSTGGMLEAARATRARQVLVATEVGMLHQLRRAAPKVDFR
                     AVNDRASCKYMKMITPAALLRCLVEGADEVDVEPDVARLARRSVQRMIEIGQPGGGE"
     gene            1735..>2182
                     /locus_tag="VF874_01570"
     CDS             1735..>2182
                     /locus_tag="VF874_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9174819.1"
                     /translation="MPHISTEHEVLAVVFQVRHLGTPNPQLSVLLWERAMNPQRGAWS
                     LPGGSLRNDEDMTSSIRRQLAEKVDLREIAHLEQLAAFSDPVRVPGARTIASTFLGLV
                     PSPATPTLPADTRWHPVDALPPMAFDHGPMVSHAHSRLVAKMSYTNI"
BASE COUNT          340 a          748 c          723 g          371 t
ORIGIN      
        1 aaagcatcgt cggatggaac tggacgaact cgatgtcgct caccggcacg cccgcccaca
       61 gggcgagcgc gacgccgtcg cctgtcgacc cttccgggtt ggtggtggca cggtacaggt
      121 tgccgagtcc gccagtcgcg aggatcacgg cgggggcatg gacgatgccg acgccgtcgt
      181 cgctgatcac ttcgacgccg gtgatgccat cgttgtcgta gagaagttgt cgcgcaacgt
      241 gattgcggcg gatgtcaagg attgcggcgg cgtgatccag cgcgcgctgc acctcggcgc
      301 cggttgcgtc tccgcccgca tggatgatgc gtcgccgcga gtgcccgcct tcacgggtca
      361 acgcccagcg cccgggcgcc gtttcgtcga aacgcgctcc gtcagaaact aattcggaaa
      421 ccgcgcggta gccatcggcg acgatcgagc gcaccgcgtc gggatcgcac aatccgcctc
      481 cggcggccac ggtgtcgttg acgtgggcat cgatcgagtc cgaggtgcgt ggcagcacga
      541 ccgcaatccc gccctgcgcg tagaacgttg cggtgtctgc cgtcttgctc agcacgatca
      601 cccggctgcc gcgccggtga gcggccagcg ctgcggccag acccgcgacc ccggtgccga
      661 tcaccacaac atcggcacgg tcctgccact tcattcgccg ccgccgggct gcccgatctc
      721 gatcatgcgc tgcacgctgc gccgagccag ccgcgcgacg tccggttcga cgtccacctc
      781 gtcggcgcct tcgacaaggc agcgcagcaa cgcggccggg gtgatcatct tcatgtactt
      841 gcaagacgcc cgatcgttga cggctcggaa gtcgactttt ggtgcggccc tgcgcaattg
      901 gtgcagcatg ccgacctcgg tggcgaccag cacctgccgg gcacgagtgg cgcgagcggc
      961 ctcgagcatg ccgcccgtcg acaggatctt gacccgctcg gcgggcaccg cgccttcccc
     1021 ggccaagtac agcgccgacg tcgcgcaccc gcattcgggg tgcacgtaca actcggcgtc
     1081 ggggtgcgag cgcgcctggt cggcgagttc gtcgccgttg atgcccgcgt gtacgtggca
     1141 ttcacccgcc cacacgtgca ggttcttgcg acccgtcacc cggcggacat gggcaccgag
     1201 gaactggtcc gggcagaaca gcacctcgcg atcctcgggg atcgacgcga ccacgtcgac
     1261 ggcgttggcc gacgtgcagc agatgtcggt gagcgccttg accgccgcgg tggtgttgac
     1321 gtacgacacc acgacggcgc cgggatgctc ggccttccac gccgcgaggt cgtcggcggt
     1381 gatcgagtcg gccagcgagc accccgcgcg ctggtcgggg atcagcacgg tcttctccgg
     1441 gctgaggatc ttggcggtct cggccatgaa gtgcacgccg cagaacacga tcgtctgttc
     1501 ggccgcttcg gcggcgatgc gcgacagggc cagcgagtcg ccgacgtggt cggcgacgtc
     1561 ttgaatggcc gggatctggt agttgtgcgc gagcagcgtt gcattgcgca gttcggcgag
     1621 ccggcgcacc tcggcggccc actcggcatc cggctcgacg ccggtataca ccccgtgatc
     1681 caggaccgtc atcgcgtctc cttcagcgct agttttcgac ttataatcga aaacatgccc
     1741 catattagca ccgagcatga ggtgctcgcc gtcgtgttcc aggttcgtca tctgggcacg
     1801 ccgaatcccc agctcagtgt gctgctgtgg gaacgggcga tgaatccgca gcgtggcgca
     1861 tggtcgcttc ccggtggcag cctgcgcaac gacgaggaca tgaccagttc aattcgtcgt
     1921 caactggccg agaaggtgga cctgcgtgag atagctcact tggagcagtt ggccgcgttc
     1981 tccgacccag tgcgggtgcc gggcgcccgg accatcgcct cgacgttcct cggcctggtg
     2041 ccctcccccg cgacgccgac cttgcctgcg gacacccgct ggcacccggt cgacgcgctg
     2101 ccgccgatgg cgttcgacca cggtccgatg gtcagtcacg cccacagcag gctggtcgcc
     2161 aagatgtcgt acaccaacat cg
//
LOCUS       DASWBF010000055         5756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_201958,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000055 DASWBF010000000
VERSION     DASWBF010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201958"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VF874_01575"
     CDS             complement(<1..1088)
                     /locus_tag="VF874_01575"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003908468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9174820.1"
                     /translation="MKQHRRRAAIPALDGVRAIAVALVLLSHGGIPGVAGGFIGVDVF
                     FVLSGFLITSLLLDELDFTGRIDLPGFWRRRAKRLLPALVLMVLAVAAVSTLFPPDAV
                     ARLRDDAVAAFVWVANWSFIARETDYFTEGGTPSPLQHTWSLGVEEQYYILWPLLLIA
                     TAAVLGVLARRRRRLLMQNTVHLAVFALAAAGVVASVVASVVMVSDSALNRVYFGTDT
                     RAQALLIGAAASALLVRSWPTLTAGLYVVRRFKRAARVVPVLGLAGLAVGAHYATGSV
                     GDFRHGMLLLVALASVAVIAPVALDQEGFVARLLAVKPLVWLGAISYGVYLWHWPVFL
                     LLNGERTGWSGLPLFVVRCVVTLGLAAAS"
     gene            complement(1205..2653)
                     /locus_tag="VF874_01580"
     CDS             complement(1205..2653)
                     /locus_tag="VF874_01580"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9174821.1"
                     /translation="MDTAKLLSSVPTGVWIGGEERQGAKTFDVLDPSDDHVLVSVADA
                     TADDAKAALDAAAGAQAEWAATPPRKRGEILRAVFEAITARADDVATLMTLEMGKVLP
                     ESKAEVAYGAEFFRWFSEEAVRYHGRFAPSPAGTGRILVTKQPVGPCYAITPWNFPLA
                     MGTRKIGPALAAGCTMIVKPARETPLTMLLLAKLTDEAGLPEGVLSVLPTTDAGGVTE
                     ALIDDGRLRKLTFTGSTGVGKRLVKQGADKLLRMSMELGGNAPFVVFDDADVDAAVEG
                     AILAKMRNGGEACTAANRFHVANAVREEFTEKLVKRMSEFTLGNGLDSSSSLGPLINA
                     DQREKVEELVDDAVSKGATVAIGGKTPDGPGHFYPATVLTEVPLDARILREEVFGPVA
                     PIVGFDTEEDGVAAANDTEYGLAAYIYTQSLDRALRVSEAIESGMVGVNRGVISDAAA
                     PFGGVKESGFGREGGFEGIEEYLDIKYIALTK"
     gene            2696..3607
                     /locus_tag="VF874_01585"
     CDS             2696..3607
                     /locus_tag="VF874_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9174822.1"
                     /translation="MAVIAESTRIGACEAYPFPGYPESLGWPPWTVPTGRIIDLPGRG
                     STYFVDSVPADGPTYVLLHSLACTGLMTWCPVLDVVRQFGRVVVFDQRGHGQGISSPR
                     FLLEDCADDVVAVADALGLRTFVPVGYSMGSMVAQLVWKRHRQRVDGLVLCAASASVT
                     RASYERLATGIFAALIEAFTPVPRPPSQMPCVEEPVRGDHQWVFDQFRSTAPAAITRA
                     IAELVRFDSRSWITDVDVPTSVVITMRDRVFSAQRQQSLADQIPGAHTVTVDAGHARC
                     TLQPDKFGPGLHQAVDSVHRRAVALSA"
     gene            3647..5314
                     /gene="pgi"
                     /locus_tag="VF874_01590"
     CDS             3647..5314
                     /gene="pgi"
                     /locus_tag="VF874_01590"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX9174823.1"
                     /translation="MTASAAQIPDVTASSAWNALQRHHDQIATKHLREFFTEDPARGG
                     ELTVSVGDLYIDYSKHRVTRETLSLLVDLARAADLERRRDEMFSGVHINTSENRAVLH
                     VALRLPRDSSLVVEGRDVVADVHDVLDRMGDFTDRLRSGEWTGATGERITTVVNIGIG
                     GSDLGPVMVYQALRHYADAGISARFVSNLDPADLVAVLADLDPAKTLFIIASKTFSTL
                     ETLTNATAARRWLTEALGDAAVSKHFVAVSTNRKLVDDFGINTDNMFGFWDWVGGRYS
                     VDSAIGLSVMAAIGRTRFAEFLTGFHTVDEHFRTAPLDANAPVLLGLLGIWYNNFFGA
                     ETRAVLPYSNDMARFAAYLQQLTMESNGKSVRADGTPVTCSTGEIFWGEPGTNGQHAF
                     YQLLHQGTRLIPADFIGFSRPTDDLATADGRGSMHDLLMSNFFAQTQVLAFGKTAAEI
                     AEEGTPPAVVPHKVMPGNRPSTTILATELTPSVVGQLIALYEHIVFTEGVIWGIDSFD
                     QWGVELGKTQAKALLPVITSDASPAQQSDSSTDALVRRYRTERGRST"
     gene            5361..5624
                     /locus_tag="VF874_01595"
     CDS             5361..5624
                     /locus_tag="VF874_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9174824.1"
                     /translation="MTSGSIPVSELREQLAAVIDELGVHGKAIKLTRHGQPVAVLMGF
                     EAFEALLDDLDDADDYRALAEHDAADEHDTIPWELVKHDLGIA"
BASE COUNT          967 a         1962 c         1919 g          908 t
ORIGIN      
        1 gacgccgcgg ccaaccctag agtgacgacg catcgcacga cgaacagcgg taggccagac
       61 caccccgtcc gctcgccgtt cagcaggagg aacaccggcc agtgccacag gtacacgccg
      121 taggagatcg cgcccagcca caccagcggc ttgaccgcca gcagacgggc gacgaagccc
      181 tcctggtcca gcgccaccgg ggcgatcacc gcaaccgacg ccagcgcgac cagcagaagc
      241 atgccgtggc ggaagtcgcc gacgctcccg gtcgcgtagt gcgcccccac ggccaggccg
      301 gccagaccca gcaccggcac gacgcgggcc gcgcgtttga accgtcggac cacgtatagc
      361 cccgcggtca gcgtcggcca actccgcacc agcagcgccg aggcggcggc gccgatcagc
      421 agtgcctgcg ccctggtgtc ggtgccgaag tagacgcggt tgagcgcgct gtcggacacc
      481 atcacgacgg acgcgacgac ggacgcgacg acacccgcgg cggccagcgc gaacaccgcc
      541 agatgcacgg tgttctgcat gagcagccgt cggcgccttc gcgcgagcac gccgagcacg
      601 gccgccgtgg cgatcagcag cagcggccac agaatgtagt actgctcctc cacgcccagc
      661 gaccaggtgt gctgcagcgg ggacggcgtg ccgccctcgg tgaagtagtc cgtctcgcgg
      721 gcgatgaagc tccagttcgc gacccagacg aacgcggcga cggcgtcgtc acgcagcctg
      781 gccacggcgt cgggaggaaa caacgtgctg acggccgcca ccgccagcac catgagcacc
      841 agcgcgggca gcagccgctt cgcgcgccgc cgccagaatc cgggcaggtc gatgcggccg
      901 gtgaagtcga gttcgtcgag cagcagcgag gtgatcagga agccgctgag cacgaagaag
      961 acgtcgacgc cgatgaaccc gcccgctaca ccggggatgc cgccgtggct gagcagaacc
     1021 agcgcgacgg cgatcgcccg gacaccgtcg agcgcgggaa ttgcggccct ccgacggtgc
     1081 tgcttcaccc ggaagagctt aagttgcgcg acgcgattcc ccggttccgc aacgccgata
     1141 ctgaagcctg ggtcgtgatc gggcggcaat cacgacctgg aattcggttt cggcgaagcg
     1201 ggcgctactt cgtcagcgcg atgtatttaa tgtccaggta ctcctcgatg ccttcgaagc
     1261 cgccctcgcg accgaaaccg gactccttga cacccccgaa cggcgcggcc gcgtcggaga
     1321 tcaccccgcg gttcacgccg accatcccgg actcgatcgc ctccgacacc cgcaacgccc
     1381 ggtccagcga ctgcgtgtag atgtaggcag ccagcccgta ttcggtgtcg ttggccgcgg
     1441 cgacgccgtc ttcctcggtg tcgaagccga cgatcggcgc gacgggcccg aacacctcct
     1501 cacgcaggat ccgcgcatcc aatggcacct cggtgagcac ggtcgccggg tagaagtggc
     1561 ccggcccgtc gggggtcttg ccgccgatcg cgacggtggc gcctttcgac accgcgtcgt
     1621 cgaccagctc ctcgaccttc tcccgctggt cggcgttgat cagcggcccc aggctcgacg
     1681 acgagtccag cccgttgccc agcgtgaact cgctcatccg cttgaccagc ttctcggtga
     1741 actcctcgcg gaccgcgttg gcgacgtgga accggttggc ggccgtgcac gcctcgccgc
     1801 cgttgcgcat cttcgccagg atcgcgccct cgacggccgc gtcgacgtcg gcgtcgtcga
     1861 acaccacgaa cggtgcgttg ccgccgagct ccatcgacat ccgcagcagc ttgtcggcgc
     1921 cctgcttcac cagcctcttg ccgacgcccg tcgacccggt gaacgtcagc ttgcgcagcc
     1981 gaccgtcgtc gatcagggcc tccgtcacgc cgccggcgtc ggtggtcggc agcaccgaca
     2041 gcacgccctc gggcaggccg gcctcgtccg tcagcttggc cagcagcagc atcgtcaacg
     2101 gcgtctcccg cgcgggcttc acgatcatcg tgcagcccgc ggccagcgcc ggcccgattt
     2161 tgcgggtgcc catcgccagc gggaagttcc acggggtgat cgcgtagcac ggcccgacgg
     2221 gctgcttggt gacgaggatg cgccctgtgc cggccgggct gggcgcgaag cggccgtggt
     2281 agcgcacggc ctcctcggaa aaccagcgga agaactcggc gccgtaggcg acctcggctt
     2341 tgctctcggg caggaccttg cccatttcca gcgtcatcag cgtggcgacg tcgtcggcgc
     2401 gggcggtgat cgcctcgaac accgcccgca agatctcgcc gcgcttgcgg ggcggggtgg
     2461 cggcccattc ggcctgcgca cccgcggccg cgtcgagagc ggccttcgcg tcgtcggcgg
     2521 tggcgtcggc gacggagacg agcacgtggt cgtcggacgg atcaagcacg tcgaaggtct
     2581 tcgcgccctg ccgttcctca ccgccgatcc acacgccggt gggaacggac gacaacagct
     2641 tggcggtatc catacacaga catttactcc tttggccgtc ggcacgcaca gccgcgtggc
     2701 tgtaatcgcg gaatcgacac gaatcggcgc ctgcgaggca tatcccttcc ccggctaccc
     2761 agaatcgctg ggttggccac cgtggaccgt gccgacgggc cggatcatcg acctgcccgg
     2821 cagaggcagc acgtacttcg tggactccgt acccgctgac gggccgacgt acgtgctgct
     2881 gcactcactg gcctgcaccg ggctgatgac gtggtgcccg gtcctggacg tcgtgcgcca
     2941 gttcggccgg gtggtggtgt tcgaccagcg cgggcacggc cagggcatct cgtcgccgcg
     3001 gtttctgctc gaggactgcg ccgacgacgt cgtcgcggtg gccgacgcgt tgggtctgcg
     3061 cacgtttgta ccggtcggct actcgatggg ctcgatggtc gcgcagctgg tgtggaagcg
     3121 gcaccggcag cgggtcgacg ggttggtgct gtgcgcggcc agcgcgtcgg tcacccgcgc
     3181 gagctatgaa cgcctggcca ccggcatctt cgcggcgctg atcgaggcat tcaccccggt
     3241 gccgcggcca ccctcgcaaa tgccatgcgt cgaggagccg gtccgcgggg atcaccagtg
     3301 ggtgttcgac caattccgca gcaccgcccc tgccgcgatc acccgcgcga tcgccgagct
     3361 cgtcaggttc gactcccggt cgtggatcac tgacgtggac gtgccgacgt cggtggtgat
     3421 cacgatgcgt gaccgcgtgt tctcggccca gcgtcagcag tcgttggccg accagatacc
     3481 gggcgcacac acggtgacgg tcgacgcggg ccacgcccgg tgcacactgc agcccgacaa
     3541 gttcgggccg ggacttcacc aggcggttga ttcggttcac cgacgggccg tggccttaag
     3601 cgcgtgaagc gactcagcac cgggcggcac gcactagggt cgaaatatga ccgcctcagc
     3661 cgcgcaaatc cctgacgtca ccgcctcatc cgcgtggaat gccctgcagc gtcatcacga
     3721 tcagatcgcc accaaacacc tgcgcgagtt cttcaccgag gacccggcgc gcggcggcga
     3781 attgaccgtc agcgtcggcg atctctacat cgactacagc aagcaccggg tcacccgaga
     3841 gacgctgtcg ctgctcgtcg atctcgcgcg ggcggctgac ctggagcgcc gccgcgacga
     3901 gatgttctcc ggcgtgcaca tcaacacttc cgagaaccgt gcggtgctgc acgtggcgtt
     3961 gcgtctgcct cgggactcct cgcttgttgt cgaggggcgc gacgtcgtcg ccgacgtgca
     4021 cgacgtgctg gaccggatgg gcgacttcac cgaccggctg cgcagcggcg agtggaccgg
     4081 cgccaccggc gagcggatca ctaccgtcgt caacatcggc atcggcggct cagacctcgg
     4141 accggtgatg gtgtaccagg cgctgcggca ctacgccgac gcgggcatct cggcacggtt
     4201 cgtgtccaac ctcgacccgg ccgatctggt ggcggtactg gcggatctgg acccggccaa
     4261 gacgctgttc atcattgcgt cgaagacgtt ctcgacgttg gagacgctga cgaacgcgac
     4321 cgcggcgcga cggtggttga ccgaggcttt gggcgacgcc gcggtgtcca agcatttcgt
     4381 cgcggtgtcg acgaaccgca agctcgtcga cgacttcggc atcaacaccg acaacatgtt
     4441 cggcttctgg gactgggtcg gcggccggta ttcggtggac tcggcgatcg ggctgtcagt
     4501 gatggcggcg atcgggcgta cgcgtttcgc agagtttctg acgggctttc acaccgtcga
     4561 cgagcacttc cggacggcgc cgttggacgc gaatgcacct gtgctgctgg ggcttttggg
     4621 tatctggtac aacaacttct tcggcgcgga gactcgcgcg gtgctgccgt actccaacga
     4681 catggcccgc ttcgcggcgt atctgcagca gctgacgatg gagtccaacg gcaagtcggt
     4741 gcgggcggac gggacgccgg tgacgtgttc taccggtgaa atcttctggg gcgagccggg
     4801 aaccaacggc cagcatgcgt tctatcaact gctgcaccag ggcacgcggc tgattccggc
     4861 cgacttcatc ggattcagca ggcccacaga cgatttggcg accgcagacg gtcgcggcag
     4921 catgcacgac ctgttgatga gcaacttctt cgcgcaaacg caggtgctgg cgttcggcaa
     4981 gacggcggcc gaaatcgccg aggagggaac acctcccgcc gtggtgccgc acaaggtgat
     5041 gccgggcaac cggccgtcga cgacgatcct ggcgaccgag ttgacgccgt cggtggtggg
     5101 ccagctgatc gcgctgtacg agcacatcgt gttcaccgag ggcgtgatct ggggcatcga
     5161 ctcgttcgac cagtggggtg tggagctggg caagacgcag gccaaggcgc tgctgccggt
     5221 gatcacgtct gacgcgtcgc cggcgcagca gtccgacagc tcgaccgacg cgctggtgcg
     5281 ccgctaccga acagaacgcg gccgctcgac ttagttttcc gcgagccgag cgttctgtac
     5341 aacgttgtac aattgtcgcc atgacgtcag gcagcattcc cgtatcggaa ctgcgcgaac
     5401 aattggccgc ggtgatagac gaactcggcg tgcacggcaa agcgatcaag ctgacgcgcc
     5461 acggccaacc tgtcgcagtg ctgatgggct ttgaagcatt cgaggcgctg ctcgacgatc
     5521 tcgatgacgc cgacgactac cgcgccctag ccgaacacga tgcggccgac gaacacgaca
     5581 caatcccttg ggaattggtg aagcacgatc tcggaatcgc gtgacctatc gggtccacaa
     5641 gacgcatcgg gttgaccgcg agctgcgagg gattcagaag cgcgaccgac tccgtatcga
     5701 aggtgtcgtt gcattgctgg ccgagaatcc tcgaccgccc aaggcgacga agctcg
//
LOCUS       DASWBF010000056        12298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1252653,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000056 DASWBF010000000
VERSION     DASWBF010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12298
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252653"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /gene="treY"
                     /locus_tag="VF874_01600"
     CDS             <1..1601
                     /gene="treY"
                     /locus_tag="VF874_01600"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX9174825.1"
                     /translation="VDGIRVDHPDGLSDPTGYLTWLREITGPQTWIVIEKILAVDEPL
                     DPTLPVAGTTGYDALREIGGLLVDPTGEPALTALSDSAGTDYAAMPTLARELKAAAVT
                     GTLASELRRLCRAIVRVTGGDHPQLPGAVAALISRIPVYRSDYVCLSSVMPTALAETI
                     EAAPELAVPLEIVAAATSFDEPAVRLQQLCGAATAKSMEDCLFYRDERLVSLNEVGGE
                     PTRFGVSAAEFHQRAADRARLWPSAMTTLSTHDTKRGEDVRARIGVLSQVPSLWAEFV
                     GGWEQRAACPDPATGLFLWQNIFGVWPVNGTVTPELRRRLHGYAEKAIRESASHTSWN
                     DPDEHFESAVHRWLDTVIEGDIAAELTGVVARLDPHARNDGLAQKLLQLTAPGVPDVY
                     QGTELWEDSLVDPDNRGPVDYAARRVALTDLDHPKMRVTTAALRLRRRRPDTFLAGGY
                     RPVLASGQASNHVVAFSRGDDVAVAVSRWTLQLEEIGWGDTELSLPTSRWTDVLTGRQ
                     WEHRVSAVELFGELPVALLERTDA"
     gene            1594..3357
                     /gene="treZ"
                     /locus_tag="VF874_01605"
     CDS             1594..3357
                     /gene="treZ"
                     /locus_tag="VF874_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX9174826.1"
                     /translation="MPDSLRASGSSQPEFAVWAPLPDQVRLDVDGTLHPMRRGDDGWW
                     RATVDAAPDARYGFVLDDDPTVLPDPRSPRQPDGVHERSQLWSPSPDAWTDQDWRGRS
                     IEGAVVYELHIGTFTDEGTLDSAIGKLDYLVDLGVEFVELMPVNAFAGTHGWGYDGVL
                     WYAVHEPYGGPDALVRFVDACHRRGLGVLIDAVFNHLGPSGNYLPRFGPYLSSGSNPW
                     GEGLNLSDADADEVRRYIIDCALSWMRDFHADGLRLDAVHALVDTTAIHILEELSAET
                     DALAEQLGRPLSLIAESDPNDPRLITPREAGGYGMTAQWDDDIHHAIHAAVSGERQGY
                     YGDFGSLETLATTLRNGYFHAGTYSSFRGRRHGRPLDKNAIPATRLMAYTCTHDQVGN
                     RAVGDRPSQNLDFGQLAVKAALALGSPYTAMLFMGEEWGSSRPFQFFTSHPESELARA
                     TAEGRKAEFAHHGWDADDMPDPQDPQTFSRSKLDWDEAGIGDHARLWEIYQRLIALRH
                     AEPDLAVPWLEHLSVEYDEDKQWVVMRRGALSIACNIGTNPVAVPVSGEVVLAWGEPV
                     VEGSRTTLPGHSFAIVRTVKS"
     gene            3434..3718
                     /locus_tag="VF874_01610"
     CDS             3434..3718
                     /locus_tag="VF874_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174827.1"
                     /translation="MKALSQVRLGPRPKRPLLAATMVALLDVLKGLKQGSTVALLLTR
                     PGKGPVSYADRQWATLLAEVAAEIGVPIEPVFRANDESLVQVETTIRAAS"
     gene            3736..4002
                     /locus_tag="VF874_01615"
     CDS             3736..4002
                     /locus_tag="VF874_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9174828.1"
                     /translation="MERIGLRELRQNASRYIARVAQGETVEVTQRGRLVARIVPAVDD
                     PWEDLILRGEVIPATSSEDLLDEAPVDYGFDASAALQRLRADER"
     gene            4002..4412
                     /locus_tag="VF874_01620"
     CDS             4002..4412
                     /locus_tag="VF874_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9174829.1"
                     /translation="MQVYLDTSALLKLIVKEPESSALETYLGGFQSDTRFTAALARAE
                     LVRAVARRESTASVEHARRVLGRLDLVPLTNRLLDAAAAMTPQALRTLDAIHLAAAMT
                     APDLRAVVTYDRRLADAAAAHGIPAVNPGVSTTG"
     gene            complement(4402..5715)
                     /gene="ilvA"
                     /locus_tag="VF874_01625"
     CDS             complement(4402..5715)
                     /gene="ilvA"
                     /locus_tag="VF874_01625"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEX9174830.1"
                     /translation="MSAEVNLIDRDGPLCAADIDAAVKRISGVVAPTPLQLSERLSAM
                     TGATVYLKREDLQSVRSYKLRGAYNLLMQLSDEERVAGVVCSSAGNHAQGFAHACRSM
                     QINGRVYVPRKTPKQKRDRIRYHGGEFVELIAVGSTYDLAAAAALEDVARTGATLVPP
                     FDDLRTMAGQGTIAAEILDQLDVGAPPACGGEPDLVIVPVGGGGCIAGITTYLAERTS
                     NTSVLGVEPAGAAAMMAALAAGEPVTLEHVDQFVDGAAVNRAGTLTYAALAAAGDMVS
                     VTTVDEGAVCTAMLDLYQNEGIIAEPAGALSVTALRENDVVEPGSTVVCLISGGNNDV
                     SRYGEILERSLVHLGLKHYFLVDFPQEPGALRRFLDQVLGPNEDITLFEYVKRNNRET
                     GAALVGIEMGSAEDYDGLLTRMEASDMEVEVLAPDSPAYRYLINP"
     gene            complement(5753..6190)
                     /locus_tag="VF874_01630"
     CDS             complement(5753..6190)
                     /locus_tag="VF874_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9174831.1"
                     /translation="MPLSGEYAPSTSDWARENAEKYMESGGTEGTLMQGKPVILLTTV
                     GAMSGKLRKTPLMRVSHNGEYAIVASLGGAPKHPVWYHNVKANPRVELQDGAETHDYE
                     AREVFGDEKAVWWERAVQAWPDYANYQKKTDRQIPVFVLTRVD"
     gene            6279..6503
                     /locus_tag="VF874_01635"
     CDS             6279..6503
                     /locus_tag="VF874_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174832.1"
                     /translation="MTTVEHIRSIASSNTVTIAGVPWPRYKVLALLVSLLVALVVGVV
                     TMSTGAAVLTGAGAGTLVWLALGSAQNYRH"
     gene            complement(6629..8425)
                     /gene="fadD11"
                     /locus_tag="VF874_01640"
                     /pseudo
     CDS             complement(6629..8425)
                     /gene="fadD11"
                     /locus_tag="VF874_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase FadD11"
     gene            8450..9205
                     /locus_tag="VF874_01645"
     CDS             8450..9205
                     /locus_tag="VF874_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174833.1"
                     /translation="MDNSGEQQRSHEDVVRRTEQLAKEAEQRLPAWLRPGNPESRWPV
                     LAALITAIVMQRAIPERYTVVPRWPLVAMEALLVVVLLVTNPNRLTRPTKVGKAASLL
                     LLAAITIDNTASAIVLDYHILTGKVSNDPAVLLGSGAAVFITNIIVFGIWYWEIDLGG
                     PFARAGVKERDPKRVYPDFMFPQMDDRRLAPPDWEPRFFDYLYVSLTNVMAFSPTDTM
                     PLTHRAKAMMTVQALVAVSTIALVFARAVNVLS"
     gene            9217..10050
                     /locus_tag="VF874_01650"
     CDS             9217..10050
                     /locus_tag="VF874_01650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9174834.1"
                     /translation="MRIDASGLTPIEQTAFLTLYARALDSRWHHPILGDALADETAGK
                     IDYDFDGIGIVSSVVCQTALRAKMLDERANQFVRKHPDAVVVDLGAGLDSAVFRVDPP
                     PTVDWYSVDLPGVISLRDETLPARPDAHSVAASLAEPHWADAIPGNRPTFLFADGLLA
                     FLSEPVIIALFRSLTDQFRSGELAFNDYGHIGWFSKLAVQLAPQKMFNDVAMQWGYPG
                     FKDAHHPESWNPKLRLVEETSLAGQPEVDLFPAWLRVSTRISARIPAMARKARILRYR
                     F"
     gene            complement(10070..>12298)
                     /gene="dnaE"
                     /locus_tag="VF874_01655"
     CDS             complement(10070..>12298)
                     /gene="dnaE"
                     /locus_tag="VF874_01655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9174835.1"
                     /translation="CQDGRDRVIEYVKKKYGAQSVSQIATFGTMAAKAVVRDVGRVMD
                     MSYGEVDRIAKMIPFDLKMTLDKALEESPPLQDAYAKDPRVKELIDVSRRLEGTTRHA
                     STHAAGVVIAPQPLTELVPLFRGNTADVTTQFDMKGVERIGLLKMDFLGLRNLTIIGD
                     AIDNIKANKGIDVDLESLPLDDKPTFELLGRGDTLGVFQLDGGPMRDLLRRMQPTGFE
                     DIVAVIALYRPGPMGQNAHNDYADRKNGRQSVKPIHPELAEPLLEILAETYGLIVYQE
                     QIMRIAQKVSGYSLARADILRKAMGKKKREVLDKEREGFAEGMRANGFSAGAIKALWD
                     TVLPFADYAFNKSHAAGYAMVSYWTAYLKANYPAEYMAGLLTSVGDDKDKAAVYLADC
                     RKLGITVLPPDVNESGLNFASVGADIRYGLGAVRNVGANVVGSIIRTRGDQGKFTDFS
                     DYLNKIDIAGCNKKVTESLIKAGAFDSLGHPRKGLFLVHTDAVDSVLGTKKAEAMGQF
                     DLFGAGDATTDAVFSIKVPDDDWDDKHKLALEREMLGLYVSGHPLNGVAHLLAGQVDS
                     QIPAILDGDVPNETRVRVGGILASVNRRVNKNGLPWASAQLEDLTGGIEVWFFPHTYS
                     TYGTEIVDDAVVLVSARVKIQDDRIRLVADDLVVPDFSNAVANRPLAVSLPTRQCTLD
                     KVTALKQVLARHPGTAQVHLRLVSGDRVTTLELDQSLRVTPSSALMGDLKALLGPGCL
                     SS"
BASE COUNT         2038 a         4116 c         3977 g         2167 t
ORIGIN      
        1 aggtcgacgg catccgtgtg gaccatcccg acggcctctc ggatcccacc ggctacctga
       61 cctggcttcg ggagatcacc ggcccgcaga cgtggatcgt catcgagaag atcctcgccg
      121 tcgacgaacc gctggatccg acacttccgg tggcgggcac caccggatat gacgcgctgc
      181 gcgaaatcgg tgggctattg gtcgatccca ccggcgaacc ggcgctcacc gcgctgtccg
      241 attccgccgg gaccgactat gcggcgatgc cgacacttgc ccgcgaactg aaggccgcgg
      301 cggtgacggg cacgctggcc agcgaactgc gccgactgtg ccgggccata gtgagagtga
      361 cgggcgggga ccacccgcaa ttgcccggcg cggtggccgc gctaatcagc cggatccctg
      421 tctatcgttc cgactacgtg tgcctgtcgt cggtgatgcc gacggctctc gccgaaacca
      481 tcgaggccgc accggaactc gcagtgccgc tggagatcgt cgcggccgcg acgtccttcg
      541 acgaacccgc cgtgcgtctg cagcagctat gcggcgcggc gacagccaag tcgatggagg
      601 actgcctgtt ctaccgggac gagcgactgg tgtcgctcaa cgaggtcggc ggcgaaccga
      661 cccggttcgg ggtgagcgcg gctgaatttc atcaacgcgc ggccgaccgg gcgcggttgt
      721 ggccgtcggc gatgacgacg ctgtccaccc acgacaccaa acgcggggag gacgtgcggg
      781 cccggatcgg ggtgctgtcg caggtgccgt cgctgtgggc ggagttcgtc ggcggctggg
      841 agcaacgcgc ggcgtgcccc gaccccgcga ccggattgtt tctgtggcag aacatcttcg
      901 gcgtctggcc cgtcaacggc accgtgaccc ccgaactgcg caggcgactg catggctacg
      961 cggagaaggc aatccgcgag tccgcctcgc acacgtcgtg gaacgacccc gacgagcact
     1021 tcgagtctgc ggtgcaccgt tggctggaca ccgtcatcga gggcgacatc gccgcggagc
     1081 tcacgggtgt cgtcgcgcgg ctcgacccgc acgcccgcaa cgacgggctg gcgcagaagc
     1141 tgctgcagct caccgcaccc ggggtgcccg acgtgtacca gggcaccgag ttgtgggagg
     1201 acagcctcgt cgatcccgac aaccggggcc cggtcgacta cgcggctcgc cgggtcgcgc
     1261 tcaccgatct cgaccacccc aaaatgcggg tcaccacggc ggcgctgcgg ctgcggcgtc
     1321 gtcgccctga cacatttctg gccggtggct accggccagt cctggccagc ggccaggcca
     1381 gtaaccacgt cgtcgcattc agccgcggtg acgacgtcgc tgtggcggtc agtcggtgga
     1441 cgctacagct cgaggaaatc ggttggggcg acacggaact ctccctgccc accagccggt
     1501 ggaccgacgt gctgaccggt cggcagtggg agcaccgcgt ttcggcggtg gagttgttcg
     1561 gcgagttgcc ggtcgcgctg ctggagcgga cggatgcctg attctcttcg cgcaagcggc
     1621 tcatcgcagc ctgagttcgc ggtgtgggca ccgcttcccg atcaggtgag gctggacgtg
     1681 gacggcacgc tgcacccgat gcggcgcggg gacgacggct ggtggcgcgc caccgtcgac
     1741 gcggcgcccg acgcacgcta cgggttcgtc ctcgacgacg acccgacggt gctgccggac
     1801 ccgcgctcgc cgcggcagcc cgacggcgtg cacgagcggt cacagctgtg gtcgccctcc
     1861 cccgacgcat ggaccgacca ggactggcgt ggccgctcga tcgagggtgc cgtggtctac
     1921 gagttgcaca ttggcacgtt caccgatgag ggcacgctgg attcggcgat cggcaagctg
     1981 gactacctcg tcgacctcgg ggtggagttc gtcgagctga tgccggtcaa cgcattcgcg
     2041 ggcactcacg gctgggggta cgacggcgtg ctgtggtacg cggtccacga gccgtacggc
     2101 ggtccggacg cgctggtgcg gttcgtcgac gcctgccacc ggcgggggct gggcgtgctg
     2161 atcgacgccg tgttcaatca tcttggcccg tcgggtaatt acctgccccg gttcggcccg
     2221 tatctctcgt cgggcagcaa tccgtggggc gagggactca acctgtccga cgcagacgcc
     2281 gacgaggtgc gccgctacat catcgactgc gcgctgagct ggatgcgcga cttccacgcc
     2341 gacgggctga ggctcgacgc cgtgcatgcg ctggtggaca ccaccgcgat tcacatcctc
     2401 gaagagttgt ccgccgaaac cgacgcgctc gcagaacaat tgggcaggcc gctgtcgctg
     2461 atcgccgaga gcgacccgaa cgacccgcgg ctcatcacgc cgcgcgaggc cggcggctac
     2521 ggcatgaccg cgcagtggga cgacgacatc catcacgcta tccacgccgc ggtgtcgggt
     2581 gaacgtcaag gctattacgg tgatttcgga tcgctggaga cgctcgcgac tacgttgcgc
     2641 aacggctact ttcacgccgg cacgtactcg tcgttccgcg gccgccgcca cggcaggccg
     2701 ctggacaaga atgccattcc cgccacccgc ctgatggcct acacctgcac tcacgaccag
     2761 gtcggcaacc gtgcggtcgg cgaccgcccg tcgcagaacc tggacttcgg ccagctcgcg
     2821 gtcaaggccg cgctggctct cggatccccc tacaccgcaa tgcttttcat gggtgaggaa
     2881 tggggatctt cgcgcccgtt ccaattcttc acttctcacc ccgagtcgga gttggccagg
     2941 gcgacagccg agggccgcaa ggccgagttc gcccatcacg gctgggacgc tgatgacatg
     3001 ccggatcctc aagacccgca gacgttttcg cggtccaagc tcgactggga tgaagccggc
     3061 atcggtgacc atgcgcggtt atgggagatc taccaacggc tgatcgcgct gcgccacgcg
     3121 gaacccgacc tggccgtccc gtggcttgag cacctgtccg tcgagtacga cgaggacaag
     3181 cagtgggtgg tgatgcgacg gggcgccctg tcgatcgcgt gcaacatcgg gacgaatccc
     3241 gtcgcggtgc cggtcagcgg tgaggtggtg ctggcgtggg gtgagcccgt tgtcgaaggg
     3301 tctcgaacca cgctgccggg ccactcgttt gcgatcgtgc gaactgtcaa gtcttgatac
     3361 gcgaccccac gcctgtggat aacgcgttgc cgcgaaatcc cacaccggct tcgttggacc
     3421 cgaccgccgc atggtgaaag cgctgagcca ggtccggctc gggccccgac cgaagcgtcc
     3481 gcttctcgcg gcgaccatgg tcgcacttct tgacgtgctg aagggcctca agcagggaag
     3541 cacggtggct ctgctcttga cacggccagg aaagggcccg gtctcgtacg ccgatcggca
     3601 gtgggcgaca ttgctcgcag aggtcgccgc agagatcggc gtgcccatag aacccgtctt
     3661 tcgcgccaac gacgagtcgt tggtgcaggt cgaaaccaca atcagagcgg ccagttaagt
     3721 gctaccatgt gctacatgga gcggatcggg ctgcgtgagc tacgacagaa cgcgagccgc
     3781 tacatcgccc gcgtagcgca gggggaaacc gtcgaggtga cccaacgagg ccgtctggtg
     3841 gcccgcatcg tgccggccgt cgacgacccg tgggaagacc tgattctccg cggcgaggtt
     3901 attcctgcga cgtcgtcgga agacttgctc gacgaggctc cggtcgacta cggcttcgac
     3961 gcgtccgcgg cgctacagcg cttgcgcgct gacgaacgct aatgcaggtg tacttggata
     4021 cgtcagcgtt gttgaagctg atcgttaagg aaccggaatc ctcagcgctg gaaacctatc
     4081 tgggtggatt tcaatccgat acgcgattca ccgctgctct cgcccgcgcc gaactggttc
     4141 gggccgttgc gcgaagggag tcgacggcca gcgtcgagca tgcccgccgt gtcctcggca
     4201 ggctggatct cgtacctctg acgaatagac tcctcgacgc agccgccgcg atgacgccgc
     4261 aggcactcag aactcttgac gcaatccacc tcgccgctgc gatgaccgca ccagatcttc
     4321 gggcggtggt cacctatgac aggcgactcg cggatgctgc tgcagcccat gggattccgg
     4381 cggtgaatcc cggtgtgagc actacgggtt gatcaaatac cgatacgccg gcgagtcggg
     4441 tgccagcacc tcgacctcca tgtcggacgc ctccatgcgg gtgagcagcc cgtcgtagtc
     4501 ctcggccgag cccatctcga tgccgactag cgccgccccg gtttcgcggt tgttgcgctt
     4561 gacgtactcg aacaacgtga tgtcttcgtt gggccccagc acctggtcga ggaagcgacg
     4621 caaggcgccc ggctcctgcg ggaagtcgac cagaaagtag tgcttgaggc cgaggtgcac
     4681 cagcgaccgc tccagaattt cgccgtagcg ggacacgtcg ttgttgccgc cggagatcag
     4741 gcacaccacc gtcgaacccg gctccacgac atcgttttcc cgcagcgcag tcaccgacag
     4801 cgcgcccgca ggctcggcga tgatgccctc gttttggtac aggtccaaca tggcggtgca
     4861 gaccgcgccc tcgtcgaccg tcgtcaccga caccatgtcg ccggccgccg ccagcgccgc
     4921 gtaggtcagc gtgcccgccc ggttgactgc ggcaccgtcg acgaactggt cgacgtgctc
     4981 cagggtcacc ggctcgccgg ccgccaacgc agccatcatc gcggccgcgc cggcgggctc
     5041 gacgcccagc accgaggtgt tcgacgtcct ctcggcgaga tacgtggtga tgccggcgat
     5101 gcacccgcca ccgccgacgg gcacgatcac gaggtcgggt tctcccccgc aagcgggagg
     5161 tgcccccacg tcgagttgat cgaggatttc ggcggcgatc gtgccctggc cagccatcgt
     5221 gcgcaggtcg tcgaacggcg gcacgagcgt ggcgccggtt cgcgccacat cctcgagcgc
     5281 ggcggctgcg gcaaggtcgt acgtcgaacc cacagcgatc aactcgacga attcaccacc
     5341 gtggtagcgg atgcggtcgc gcttctgctt gggggtcttg cgcggcacgt acacacgacc
     5401 attgatctgc atcgaccggc acgcgtgcgc aaaaccctgc gcatggttgc cggccgacga
     5461 gcacaccacg ccggccaccc gttcctcgtc tgacaactgc atcagcaggt tgtacgcgcc
     5521 acgcagcttg taggaccgca ccgactgcag atcttcgcgc ttcagataca ccgtcgcccc
     5581 ggtcatcgcg gagagccgct cgctgagctg cagcggggtc ggcgcgacga ccccggagat
     5641 tcgcttgacc gccgcgtcga tgtcggccgc acacagcggc ccgtcgcggt cgatcaggtt
     5701 cacttcagcg gacaccggtc aatggtgcca tgcgccgagg caacaccgca ggtcagtcca
     5761 ccctagtgag gacgaaaacc gggatctggc ggtcggtctt cttctggtag ttcgcgtagt
     5821 cgggccacgc ctgcaccgcg cgctcccacc agacggcctt ctcgtcgccg aagacctcac
     5881 gcgcctcgta gtcgtgggtt tccgcgccgt cctgcaattc gacacgtgga ttggccttga
     5941 cgttgtgata ccagaccggg tgcttcgggg cgccgccaag cgaagcgacg atcgcgtatt
     6001 cgccgttgtg cgagacgcgc atcagcgggg ttttacgcag cttgccggac atggctccga
     6061 ccgtggtcag cagaatcacc ggcttcccct gcatcagggt gccctcggtt ccgccggatt
     6121 ccatgtactt ctcggcgttc tcgcgggccc aatcagacgt actcggagcg tattcaccag
     6181 atagaggcat gaccaaagat taggcgcttg acgtgcgcgc ggaatttcaa ccggcgtttc
     6241 ggttgtccga aacctaggtc agggactacc ctgaacctat gacgacggtg gagcacatcc
     6301 gttcgatcgc ctcatcgaac acggtgacga tcgccggtgt gccgtggccc cgatacaagg
     6361 tgctcgcact gctggtgagc cttctcgtgg cgctggtcgt cggcgtcgtg accatgtcca
     6421 ccggcgcggc ggttctcact ggcgccggcg ccggcacgct ggtctggctg gcgttgggca
     6481 gcgcgcagaa ttaccgccac taggctgcct cgcagtaaca cgggccacac ctgtatcgcc
     6541 gagcaaccat gtgtgtgcgt cgcagaagcg atagcgcatg cgatatcacc gccggacatc
     6601 agaggtggac gtgctgacgt cgcgctactc aagcatgtgg ctggtgcacc gacgggccgg
     6661 gttcgtcggc gtacagttcc tcgatctcgg tcgcatactt ttcggcgatc ggtcgccgtt
     6721 tgagtttcat cgtcagcgtg atctcgtcgc cgccaggctc ccagaacgtc ggtaacactt
     6781 ggaagcgttt gatctgctcg acccgggaga gcttgctgtt gccttcggcg attcccgcgg
     6841 cgatctggcc gatcacatcc ggatcggcgg cgagtgtctc cgccgatgcg tcgggcagtc
     6901 cgcgctgtgc ggcgtacgga actgccgact cggcgtcgag cacgacgagc gcggtgttgt
     6961 agggacgggc gtcgcctaac gcgatgatca ccccgatcag cgggcacgcg gccttgatcg
     7021 tgttctcgat gttggccgga gacatgttct tgccccccgc gttgatgatc agctctttct
     7081 tgcggtcgac gacgcgaaaa tagccgtcgg cgtccatgtc gaggatgtcg ccggtgtgca
     7141 gccagccgtc ggaatcgatt gcctctgcgg tcttttcggg ctccttgcgg taggccttca
     7201 tcaccagcgg accgcgaacc agaaattcgc cgtcgtcggc gatcttgctt tccagaccgg
     7261 gcagcaactt gccaacggtg ccgagccgcg catcgcgtgg atgactgacc gaggcgacgt
     7321 tgctcagctc cgacatgccc cagacctctg caatcgaata ccgatacctg caaagaaacc
     7381 caaggtctcc ttcgggatcg gcgcggcgcc cgagactgcc caccgcagtt gatccagccc
     7441 gagcttggct cgcagcttgg acagcaccag ctcgtcggcc tgcgcccatt ccgccgcgat
     7501 gtcatcgggg accgggccgt cggcaatcac agcggcggcc ttcttggccg cgaccgccag
     7561 accccactgc aggccctggc gcttcgtctc gtcgggttcg ttcgccgccg cgaattctat
     7621 tgccgccttg agcttctccc atacccgcgg caccgccccc cagatcgtcg gccgcacatc
     7681 tggcagcgcc cccgcgatcg ccctggcatc cgacaccgtg gtcacctggg ttccgaacac
     7741 ctcttgcagg tacaggcagc ccatccggtc ggcgatgtgc gccgacggaa ggaacgacgt
     7801 gatccggtca ccgaattcga tgccgagcac ctcatccaga gcgaacgcct cgaacagcag
     7861 actcgcgtgc gtcatctcca cgcccttggg gtttccggtc gccagacgtg tagatcaacg
     7921 tgatgacatc gtcggcttgc actgcgcgcc aggcggattc gaagtcgaaa tcggccgccc
     7981 cgcccgccag catgtcgtca agcgatgtcg cgccctccgg tgacccgtcg atgcaaacga
     8041 tgtgctcgag ggccgcgccg ctggccttca tccggtcgac gtactgctcc tcacagatca
     8101 ccaccttcgt cccggcgttg tcgaacagat acgtcaactg ctcgggcgcc agcgtgttgt
     8161 acaccgagaa cgatgtcgcg cccagatgct gtgcgccgac gtcgagtggg tagaactcga
     8221 cgcggttggc catcatgagc gacaccgtgt cgccgcgctg cacacccaag gccgctaacc
     8281 ccgccgcgac ccgtcgcacc tggccgacgt agtcgcgcca ggtcaaggtc tgcgcgtcgc
     8341 ccggagttcg cagggcgact gcgtccggat ctattgctgc ggttcgctga aaggcttcgc
     8401 agagagtggt cggtcgctcg gtcatgacgg gtagcgtacg gcgaagatga tggacaacag
     8461 cggagaacag cagcggtcgc acgaagacgt cgtgcgccgg accgagcagc tggcgaaaga
     8521 ggccgaacaa cgactgccgg catggcttcg acccggcaac ccggagagcc gctggcccgt
     8581 gctcgccgcg ctgatcaccg cgatcgtcat gcagcgcgcg atccccgagc gctacaccgt
     8641 ggtgccccgc tggccgctcg tcgcgatgga agcgctactg gttgtcgtcc tgttggtcac
     8701 caacccaaat cgcctcaccc gcccgaccaa agtgggcaaa gccgccagcc tgctgctact
     8761 cgccgcgatc accatcgaca acaccgcgtc cgcgatcgtg ctggactacc acattctgac
     8821 ggggaaggtg agcaatgacc ccgcggtgct actgggcagt ggcgcggcgg tgttcatcac
     8881 caacatcatc gtgttcggaa tctggtactg ggagatcgac ctcggcgggc cgttcgcccg
     8941 cgccggcgtg aaggagcggg atcccaagcg cgtctacccg gatttcatgt tcccgcaaat
     9001 ggatgaccgc cggttggcac caccggactg ggagccccgg ttcttcgact acctctacgt
     9061 cagcctgacg aacgtgatgg cgttctcccc caccgacaca atgccgttga cgcaccgggc
     9121 caaagcgatg atgaccgtgc aggcgctggt cgcggtgtcg acgatcgcgc tggtgttcgc
     9181 ccgcgccgtc aacgtgctga gctagtaggg cccgtgatga ggatcgacgc cagcggtctc
     9241 acaccgatcg agcagaccgc gttcctgacg ctgtacgccc gcgcgcttga cagccgttgg
     9301 caccatccga ttctgggcga cgcgctggcc gacgagaccg ccggcaagat cgactacgac
     9361 ttcgacggca tcggaatcgt ttccagcgtg gtctgccaga ccgcactgcg cgcgaagatg
     9421 ctcgacgagc gcgcgaacca attcgtcagg aagcatcccg acgcggtcgt ggtggactta
     9481 ggcgccggct tggatagtgc cgtgttccgg gtcgatccgc caccgacagt ggactggtac
     9541 agcgtcgacc tgccgggtgt catctcgctg cgggatgaga cgttgcctgc ccgccccgac
     9601 gcacactccg tcgccgcctc gctcgccgag ccgcactggg ctgacgcaat ccccgggaac
     9661 cggccgacat tcctgttcgc cgacggtctg ttggcgtttc tgagcgagcc agtgatcatc
     9721 gcgttgttcc gctcgctcac tgatcagttc cgcagcggcg agctggcctt caacgactac
     9781 ggccacatcg gctggttcag caaacttgcg gtccagcttg cgccgcagaa gatgttcaac
     9841 gacgtggcga tgcaatgggg ttatccgggt ttcaaggacg cgcaccaccc cgagtcgtgg
     9901 aatccgaagc tgcgattggt cgaggagacg agccttgcgg gacagccgga ggtcgatctc
     9961 ttcccggcgt ggctgcgggt cagcacccgg atctctgcgc gcatcccggc gatggcgcgc
    10021 aaggcgcgca tcttgcgcta ccggttctag agcaccaccg cgccggtgcc taactgctca
    10081 aacagcctgg gcccagcagc gccttcaggt cgcccatcag cgccgacgac ggtgtcaccc
    10141 gcaacgactg atcgagctcg agcgtcgtga cccgatcccc gctgaccagc cgcaagtgca
    10201 cctgcgcggt accggggtga cgggcgagca cctgcttgag cgcggtgacc ttgtcgagcg
    10261 tgcactggcg ggtgggcagg ctcaccgcca gcggccggtt cgctacggcg ttcgaaaagt
    10321 ccggcaccac aaggtcgtcc gcgaccaacc tgatccggtc gtcctgaatc ttcaccctgg
    10381 cgctgacgag aaccaccgcg tcgtccacga tctcggtacc gtacgtcgag taggtgtgcg
    10441 ggaagaacca cacctcgatg ccaccggtca gatcctccaa ttgagctgac gcccagggca
    10501 atccattctt gttgacgcga cggttcaccg aggccaggat tccgccgacg cggactcgcg
    10561 tctcgttcgg cacgtcgccg tcgaggatcg cgggaatctg ggaatccacc tggccggcga
    10621 gcaggtgcgc gacgccgttg agcggatgcc cggacacata cagccccaac atctcccgct
    10681 ccagggcaag cttgtgcttg tcgtcccagt cgtcgtcggg caccttgatc gagaacaccg
    10741 cgtcggtggt ggcgtcgccg gccccgaaca ggtcgaactg gcccatcgcc tcggccttct
    10801 tcgtgcccag caccgagtcc acggcatcgg tgtgtaccag gaagagtccc ttgcgcgggt
    10861 ggccgagcga gtcgaatgca ccggccttga tcagcgactc cgtgaccttc ttgttgcacc
    10921 ccgcgatgtc gatcttgttc aggtaatcgg agaaatcggt gaatttgcct tggtcgccgc
    10981 gggttcggat gatcgagccc acgacgttcg cgccgacgtt gcgcaccgca cccagtccgt
    11041 agcggatgtc ggcgcccacc gacgcgaagt tcaaacccga ctcgttgacg tccggcggca
    11101 gcaccgtgat gcccaacttg cggcagtcgg ccaggtacac cgcggccttg tccttgtcgt
    11161 cgcccaccga ggtcaacagg ccggccatgt actcggccgg atagttggct ttcaggtacg
    11221 cagtccagta cgacaccatc gcgtatccgg cggcgtggct cttgttgaac gcgtagtcgg
    11281 cgaacggcag cacggtgtcc cagagcgctt tgatggcacc ggcggaaaaa ccgttggcgc
    11341 gcatgccctc tgcgaagccc tcgcgctcct tgtcgagcac ttcacgcttc ttcttgccca
    11401 tggccttgcg caggatgtcg gcccgggcca gtgagtaccc ggagaccttc tgcgcgatgc
    11461 gcatgatctg ttcctggtag acgatcaagc cgtacgtctc ggcgaggatt tcgagcaggg
    11521 gttcggccag ctcgggatgg atcggtttga cggactggcg cccgttcttg cggtcggcgt
    11581 agtcgttgtg ggcgttctgg cccatcgggc cgggccggta cagcgcgatc accgcgacga
    11641 tgtcctcgaa accggtgggc tgcatgcggc gcagcaggtc gcgcatcgga ccgccgtcga
    11701 gttggaacac gccgagagtg tcgccgcggc ccagcagctc gaaggtcggc ttgtcgtcca
    11761 gcggcagcga ctccagatcg acgtcgattc ccttgttggc cttgatgttg tcgatcgcgt
    11821 cgccgatgat cgtcaggttg cgcaggccca ggaaatccat cttcagcagg ccgatgcgct
    11881 cgacgccctt catgtcgaac tgggtggtga cgtcggcggt gttgccccgg aacagcggca
    11941 ccagctcggt cagcggctgc ggggcgatca ccacgcccgc cgcgtgggtc gaggcgtgcc
    12001 gggtggtgcc ctccagccgc cgcgagacgt cgatcagctc cttgacccgc gggtccttcg
    12061 cgtaggcgtc ctgcagcggc ggcgactcct cgagcgcctt gtcgagggtc atcttgaggt
    12121 cgaaggggat catcttggcg atgcggtcga cctcgccgta gctcatgtcc atcacccggc
    12181 cgacgtcgcg caccaccgcc ttcgccgcca tcgtgccgaa agtggcgatc tgcgacaccg
    12241 actgagcgcc gtatttcttc ttcacgtact cgatgacccg gtcgcggccg tcctggca
//
LOCUS       DASWBF010000057         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_266185,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000057 DASWBF010000000
VERSION     DASWBF010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266185"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1615
                     /locus_tag="VF874_01660"
     CDS             248..1615
                     /locus_tag="VF874_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024488023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX9174836.1"
                     /translation="MKHTTALRQLQVSASGEGLVSRGGIALLAGTARVAGIEDELRTA
                     LAPWRRPRAVHDPATVVTQLSYALAAGGDCLADIAMLRDATAIVGAVPSDPTVSRVID
                     DLAAGGQQVLDAIADAHAAARARVHAAGGGPAGTGPDGLVPLDIDATLVSAHSDKEDA
                     RPTYKRGFGHHPVMAFLDHGAGGTGEALAGLLRPGNANANNAADLLTTLDLALTQLPD
                     TLRGQVLIRCDGGGYSHKFFAGVIDRKLEFSVGWQANLDVADAITLLPETAWTPAYDS
                     DGQPREGADVTELSGLLELTGWPAGTRVIARREVPHPGAQLRLTDVEGRRITCFATNT
                     TRGQLADLELRHRRRARVEDRIRCAKDTGLTNLPLHDTASNRVWLALVLLAVDLLTWT
                     QTLTLTGPLRAAEPKTLRHRLLAVAARIAHTGRRTHLRLDRHWPWAATLATAIATLQA
                     IPDPT"
     gene            complement(1862..>2843)
                     /locus_tag="VF874_01665"
     CDS             complement(1862..>2843)
                     /locus_tag="VF874_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018159633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX9174837.1"
                     /translation="ISTTGVGGLTLGGGIGYLDRSLGLSCDNLISADVVTADGQLVIA
                     SETQNDDLFWALRGGGGNFGVVTSFEFRLSPVKDIFGGPIFFELDRAGDLLRFYREFI
                     LDAPEQLGGFPAFQIAPPLPFIPEDRHGEPFIAFVACWAGPLDEGEKAIQPIRDVAPI
                     VAEMVGPMPYPMLNSAFDALVPPGLQHYWKANFNTELTDEAIDAHLRYGPKVPAVNST
                     MHIYPINGACHRVAGDATAFAYRDATFATVIAGMWPDPADNEANIQWVKDYYAATAPH
                     SEAGGYINFMAEDDQHRIEDNYKGNYERLVDVKRKYDPNNLFSVNQNIRP"
BASE COUNT          507 a          971 c          909 g          456 t
ORIGIN      
        1 acttcgagct gttcaccggc gcggtatttc gcgccccggt ggcgttgacg ccggggcaac
       61 gatcgggccg cacattctcc ctggatgacc cgaagctcga cgcggccagg cactaacctc
      121 gccctttcgc gtgactcgtg atcttgcggt ggtgacacgg cggtaaatgc acggaagtgc
      181 ctgtcctgca cgggaaactg agcttgtcga gagtccagtt cggtccgagc gggaaggcac
      241 tccgtaggtg aagcatacga cggcgttgcg gcagttgcag gtctcggcct ccggggaggg
      301 gcttgtgtcg cgcggcggga tcgcgctgct ggccggcacg gcccgggtcg ctggcattga
      361 ggatgagctg cgcacggcgc tggcgccgtg gcgacggccc cgcgcagtgc acgacccggc
      421 cacggttgtg acccaactca gctacgcctt ggccgccggc ggcgactgcc tggctgacat
      481 cgcaatgttg cgcgacgcga cggccatcgt tggtgcggtg ccgagtgatc cgacggtgtc
      541 gcgggtgatc gacgacctgg ccgccggcgg ccagcaggtg ctggacgcga tcgccgacgc
      601 gcacgccgca gcccgcgccc gggtccatgc cgctggcggc ggaccggccg ggaccgggcc
      661 ggatgggctg gtgccgttgg acatcgacgc cacgctggtg agcgcacact cggacaagga
      721 agacgcccgg ccgacctata agcgcggctt cggccaccat ccggtcatgg cgttcctcga
      781 ccacggcgcc ggtggcaccg gtgaggcgct ggccgggctg ctgcgaccgg gcaacgcgaa
      841 cgcgaacaac gccgccgatc tgctcaccac cctcgacctg gccctgacac agctgccgga
      901 cacgctgcgc ggtcaggtgc tgatccgctg cgacggcggc ggttactcgc acaagttctt
      961 cgccggcgtc atcgaccgga agctggagtt ctccgttggc tggcaagcca atctcgatgt
     1021 cgccgacgcg atcacactgc tgcccgagac cgcatggacc ccggcctatg acagcgacgg
     1081 acaaccccgc gagggagccg acgtcaccga actgagcggc ctgctcgagt tgaccggctg
     1141 gccagccggc acccgcgtga tcgcccgccg cgaagtgccg caccccggcg cgcagctgcg
     1201 actgactgat gtcgagggcc ggcggatcac ctgcttcgcg accaacacca cccgcgggca
     1261 actcgccgac ctcgaactgc ggcaccgtcg ccgcgcccgc gtcgaagaca ggatccgctg
     1321 cgcgaaagac accggcctga ccaaccttcc gctgcacgac actgcgtcca accgagtctg
     1381 gctcgccctc gtgctactcg ccgtcgacct gctcacctgg acccagaccc tcaccctcac
     1441 cggaccactg cgcgccgcgg aacccaagac cctgcgccac cggctgctcg ccgtcgccgc
     1501 gcgcatcgcg cacaccgggc ggcgaaccca cctgcgcctg gaccggcact ggccctgggc
     1561 agcgaccctc gccaccgcga tcgcgacact gcaggcgatc cccgacccca cctgaacctc
     1621 aacacctgac ccgacgacac gaggacccgg aaacaacgca tcggcgcgac cgccgacgac
     1681 ccgccacacc cgagcaacga aaatgcccgc cgcaccactc accgcgaccc atcaccaaga
     1741 tcatgaaaga ttgaggctaa gctcagctcg gcctatcgcg cccgtatcgc cgcaacgcct
     1801 tcggcgttgc ggcgaccggt gtgagaaata gcgggctatc gggacgcaag cgtctcgatc
     1861 atcagggcct aatgttttga ttcacactga agaggttatt cgggtcgtac ttgcgcttga
     1921 catcaactag acgctcgtaa ttgcccttgt aattgtcctc gatgcggtgc tggtcgtctt
     1981 cggccatgaa gttgatgtag ccgcccgctt cggagtgtgg tgcggtggcc gcgtagtagt
     2041 ccttgaccca ctggatgttg gcctcgttgt cagccggatc gggccacatt ccggcaatga
     2101 cggtcgcgaa agtcgcgtcg cgataggcga atgccgtcgc gtcgcccgcg acgcggtgac
     2161 aggcaccgtt gatcggatag atgtgcatgg tcgagttgac tgccggcacc ttcggcccat
     2221 agcggaggtg cgcgtcgatc gcttcgtcgg tcagttcggt gttgaaatta gccttccagt
     2281 agtgctgcag gccgggcgga accagcgcgt cgaatgcgct attgagcatg gggtacggca
     2341 tcggaccgac catctccgcc acgatcggcg ccacgtcgcg gatcggttga atcgccttct
     2401 cgccttcgtc caatgggccg gcccagcacg cgacaaacgc aatgaacggt tcgccgtgcc
     2461 ggtcttccgg aatgaacggc aatggcgggg cgatctggaa ggcagggaac ccgccgagct
     2521 gctcgggagc gtcaagaatg aattcccggt agaaccggag taggtctccc gcgcgatcta
     2581 gctcgaagaa gatcgggcca ccgaagatgt ccttgaccgg actgagccgg aactcaaacg
     2641 aggtgacgac gccgaagttg ccgcctcccc cgcgcaacgc ccagaacaaa tcgtcgtttt
     2701 gggtttcact tgcgatgacg agctgaccat ccgcagttac tacgtcggcc gagatgaggt
     2761 tgtcacaaga aaggcccaga ctccggtcta gatagccgat acccccaccg agggttaggc
     2821 cgcccacacc ggtggtcgaa atg
//
LOCUS       DASWBF010000058         4751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_466382,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000058 DASWBF010000000
VERSION     DASWBF010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466382"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VF874_01670"
     CDS             complement(<1..512)
                     /locus_tag="VF874_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HEX9174838.1"
                     /translation="MKVIVEADGGSRGNPGRAGFGSVVWSADHSTVLAEIKEAIGTAT
                     NNVAEYRGLIAGLTEAANLGASDVDAFLDSKLVVEQMSGRWKVKHPDLAQLHQEARRL
                     ASGFERISFSWIPREKNSHADHLANEAMDAAAVLEDTPSPARWTGAQGAPTRLLLLRH
                     GQTELSAQRR"
     gene            complement(509..1234)
                     /locus_tag="VF874_01675"
     CDS             complement(509..1234)
                     /locus_tag="VF874_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX9174839.1"
                     /translation="MKAAVDQQRSLADIVEIDAELALIAHRRARLPGIARYEEIQAEQ
                     RAANDRLAALQLALEDLQTQVNRFETEIDAVRQREERDRSLLASAATDAKQLNDLQHE
                     LETLQRRQASLEDSLLEVMERREELQLDQARELAAIEERHAELASVQQGLDEALAEFD
                     SITEDRGWRRAELVASLDLELVALYERQRSSAGVGAGLLQGKRCGACRIELDRGELAR
                     ISAAADDDVLRCPECRAILLRVT"
     gene            complement(1234..2358)
                     /locus_tag="VF874_01680"
     CDS             complement(1234..2358)
                     /locus_tag="VF874_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX9174840.1"
                     /translation="MSGPLRDIIDVLEQAYPPRLAQPWDSVGLVCGDPGDVVSDVVVA
                     VDATVAVAAEVPDGGLLLAHHPLLLRGVDTVAASTPKGALVHSLIRTDRALFTAHTNA
                     DSASPGVSDALAQTLGVEVDGVLSPLFDGPSLDKWVVFVPPENADAVRAAMFGAGAGQ
                     IGDYSQCSWSVTGTGQFLPHEGATPAIGSVGSVEQVTEDRVEVIAPAAARPAVLAAMR
                     AAHPYEEPAFDVFALAPLPGDAGLGRVGSLPSPEPLRTFTSRVRRALPATSWGVRAAG
                     DPDRTVSRVAVCGGAGDSLLDTVAAAGVDVYVTADLRHHPADEHRRASDVALIDVAHW
                     ASEFPWCAQAADLLRAHFGPSLTVRVSDTRTDPWNIEDVT"
     gene            complement(2355..3317)
                     /gene="cobC"
                     /locus_tag="VF874_01685"
     CDS             complement(2355..3317)
                     /gene="cobC"
                     /locus_tag="VF874_01685"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2231c family pyridoxal phosphate-dependent
                     protein CobC"
                     /protein_id="HEX9174841.1"
                     /translation="MLDFAVNVRGSAPPAWLLGRLAARLSDLGRYPSQADERAATTAV
                     AERHGRRDDEVVLLAGATEGFSLLSNLQPRLAAVLAPSFTEPEAALQAAGVPVEHVVL
                     APPFGMDAVEVPDEADLVVVGNPTNPTSVLHPREHILRLRRPGRVVVVDEAFADAVPG
                     ERESLASELLPDVLVLRSLTKTWALPGLRVGYALGARELLGRLTATRPHWPLGTLQLE
                     ALIACNDSLSVEEAEEQAAHMVTMRAEMCRGLKAVGARVVDGSAPFVLFEVPEAPLAR
                     KRLAKKRIAVRRCDTFVGLDGHYLRAAVRREWPTLVNALAEIIG"
     gene            3369..4025
                     /locus_tag="VF874_01690"
     CDS             3369..4025
                     /locus_tag="VF874_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9174842.1"
                     /translation="MTSRPQLVIFDLDGTLTDSAAGIVSSFRHALAQVGEPVPDDPDL
                     AGLVAGPPIHQTLHGLGLGEKTDAAIAAFRADYTARGWVLNEVFDEIPELLADLRAAG
                     VRLAVATSKAEPTARKILAHFGLDDHFEVIAGASLDGVRASKAEVVAHALAQLDPLPE
                     HVLMVGDRSHDVHGASHHGIDTVVVEWGYGAGDFEDPEVQRPLAQVPTVSALREVLGV
                     "
     gene            4018..4512
                     /locus_tag="VF874_01695"
     CDS             4018..4512
                     /locus_tag="VF874_01695"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX9174843.1"
                     /translation="MSDHLHVTFVCTGNICRSPMAEKMFAHQLRERGLHEHVRVTSAG
                     TGDWHVGSEADARASLVLRAHGYPTAHCAAQLDDDHLSADLVVALGHNPARMLAHLGV
                     PGERVRLLRSFDPRSGAHALDVEDPYYGAHADFQETFDVIEASLPGLHDWVDEQLAAR
                     GLVG"
     gene            4512..>4751
                     /locus_tag="VF874_01700"
     CDS             4512..>4751
                     /locus_tag="VF874_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HEX9174844.1"
                     /translation="MRRWAFVFRPGWIALALVVAAFAYLCFTVLAPWQLGKNSKTSRE
                     NDQIAASLTSDPVPVTSLLPQADSAQWRRVTATGHY"
BASE COUNT          732 a         1705 c         1585 g          729 t
ORIGIN      
        1 taccgccgct gcgcggaaag ctcggtctgg ccgtggcgca acaacaacag cctggttggc
       61 gctccttgcg cgcccgtcca gcgggccggg gacggcgtat cctcaagtac ggccgcggcg
      121 tccatcgcct cgttcgcgag atggtcggcg tgcgaattct tttcgcgcgg aatccaactg
      181 aacgagatgc gctcgaatcc tgacgcgagc cgacgcgctt cctgatgcag ctgcgcaaga
      241 tccgggtgct tgaccttcca gcgtcccgac atctgctcga cgaccagctt ggagtcgaga
      301 aacgcgtcga cgtcgctggc cccgaggttc gcggcctcgg tgagacccgc gatcagcccg
      361 cgatattcgg cgacgttgtt tgtggcggtg ccgatggcct ccttgatctc ggccaggacc
      421 gtcgaatgat cggccgacca caccaccgag ccgaagcccg cgcgaccggg gttgccgcgc
      481 gacccgccgt cggcttcgac gatcaccttc atgtgaccct caacaggatt gcgcggcatt
      541 ccgggcatcg cagcacgtcg tcgtcggccg ccgccgaaat ccgggccagc tcacctcgat
      601 ccagttcgat gcggcacgcc ccgcaccgct tgccctgcag cagccccgcg ccgacaccgg
      661 ccgacgagcg ctgacgctcg tacagcgcaa cgagttcgag gtccagactc gcgacgagtt
      721 ccgcccggcg ccagccgcgg tcctcggtga tgctgtcgaa ctcggccaat gcctcgtcga
      781 gcccctgctg caccgaggcc agctcggcgt gccgctcctc gatcgcggcg agctcgcggg
      841 cctggtcgag ctgcagctcc tcgcggcgct ccatcacctc cagcagtgaa tcctcgaggc
      901 tcgcctgacg tcgctgcaac gtctccaact cgtgctgtaa gtcgttcagc tgcttggcat
      961 ctgtcgccgc cgaggccaga agcgaacggt cccgctcctc gcgctgccgc accgcgtcga
     1021 tctccgtctc gaaacgattg acctgagtct gcagatcctc cagcgccaac tgcagcgcgg
     1081 cgagacggtc gttggccgcg cgttgctcgg cttggatctc ttcatatcgg gcgatcccgg
     1141 gcagacgcgc gcggcggtgc gcgatgagcg ccagttcggc atcgatctcg acgatgtcgg
     1201 ccagcgaacg ttgttggtca acagcagctt tcatcacgtc acgtcctcaa tgttccatgg
     1261 gtcggtgcgg gtgtccgaca cccgcaccgt cagcgagggg ccgaagtggg cgcgcaacag
     1321 gtcggccgcc tgggcgcacc acgggaattc actggcccag tgtgcaacgt cgatcagcgc
     1381 cacgtcggac gcgcgccggt gttcgtcggc cgggtggtgg cgcaggtcgg cggtgacgta
     1441 cacgtcgacc ccggcggcgg ccaccgtgtc gagcagcgag tcgcctgctc ccccgcagac
     1501 cgcgacccgc gacaccgtcc ggtccgggtc accggcggcc cgaacacccc atgacgtggc
     1561 cggaagtgcg cgccggacgc gggatgtgaa cgtgcgcaac ggttccggag acggcaggga
     1621 cccgacgcgg cccagccccg catcgcctgg cagcggcgcc agcgcgaaca cgtcgaaagc
     1681 cggctcctca taagggtgtg cagcgcgcat cgccgcgagc acggccggcc gggccgccgc
     1741 gggcgcgatc acctccaccc ggtcctcggt gacctgttcg acggatccga cgctgccgat
     1801 ggccggcgtc gcgccctcgt gcggcagaaa ctgaccggta ccggtcacgc tccaactgca
     1861 ctgggagtag tcgccgatct ggccggcacc cgcgccgaac atcgccgccc ggaccgcgtc
     1921 ggcgttctcg ggcgggacga acaccaccca cttgtccagc gagggcccgt cgaaaagcgg
     1981 tgacagcaca ccgtccacct cgacgccgag ggtctgcgcc agcgcgtcgg acaccccggg
     2041 cgacgcggag tcggcgttcg tgtgggcggt gaacaacgcg cggtccgtcc ggatcaggct
     2101 gtgcaccagc gcacccttcg gcgtgctggc tgcgaccgtg tcgacaccgc gtaacagcag
     2161 cgggtggtgt gcgagcagca gcccaccgtc gggcacttcg gcggccaccg cgacggttgc
     2221 gtcgacggcg acgacgacgt cgctcacaac gtcgccagga tccccacata ccaagccgac
     2281 cgagtcccac ggttgcgcca gccgcggcgg gtacgcctgc tctagcacgt cgatgatgtc
     2341 gcgcagcggc ccgctcatcc gatgatctcc gcgagcgcat tgaccagcgt cggccactcc
     2401 cgccgcaccg ctgcccgcag ataatgtccg tcgaggccga caaacgtgtc gcatcgacgg
     2461 accgcaatcc tcttcttggc cagccgcttt cgcgccagcg gcgcctcggg gacttcgaac
     2521 agcacgaacg gcgccgaccc gtcgaccacg cgggcaccca ctgccttaag tccgcggcac
     2581 atctccgcgc gcatcgtaac catatgcgcc gcttgctcct cggcctcctc gacggacaac
     2641 gaatcgttgc acgcgatcag cgcctccagc tgaagcgtgc cgagcggcca gtgcgggcgc
     2701 gtcgcggtca gccgtcccag cagttcccgt gcgcccagcg cgtaccccac ccgaagcccc
     2761 ggcagagccc acgttttggt gaggctgcgc agcacgagca cgtcaggcag caactcagaa
     2821 gccagcgact cccgctcgcc gggtaccgcg tcggcgaagg cctcgtcgac caccaccacc
     2881 cggccgggcc ggcgcaaccg caggatgtgt tcgcgtggat gcagcaccga cgtcgggttg
     2941 gtcgggttgc ccactaccac gaggtcggcc tcgtcgggga cctcgacggc gtccatacca
     3001 aacggcgggg ccagcacgac gtgctcgacg gggacaccgg cggcctgcag cgcggcttcg
     3061 ggctcggtga acgacggcgc gagcaccgca gccagccgtg gctggaggtt cgacaacagc
     3121 gagaagccct cggtcgcgcc ggccagaagg accacttcgt cgtcgcgccg gccgtgacgt
     3181 tcggccacgg cggtggtcgc cgcgcgctcg tccgcctggc tcgggtaacg gcccagatcg
     3241 ctcaggcgcg cggccaaccg gccgagcagc cacgccggcg gcgccgaccc ccgcacgttg
     3301 acggcgaagt ccagcatccc gggcagcgcc gcttggtcgc cgtgataccg cacagggaca
     3361 atgtacgggt gacctcccgg ccgcaactcg tcatcttcga cctggacggc acgctgaccg
     3421 actccgctgc cggcatcgtg tcgagttttc gccacgcgct tgcccaggtc ggcgagccag
     3481 tccccgatga tcccgacttg gcgggcctgg tggccggccc gccgatacac cagacgctgc
     3541 acggcctggg cctcggcgag aaaaccgacg cggcgatcgc ggcgtttcgc gccgactaca
     3601 ccgctcgcgg atgggtgctg aacgaggtgt tcgacgagat ccccgagttg ctggccgacc
     3661 ttcgcgccgc gggtgtgcgg ctggcggtgg ccacctctaa ggcggagccg acggccagga
     3721 agatccttgc gcacttcggg ctcgacgacc atttcgaggt gatcgcgggc gcgagcctgg
     3781 acggcgtccg cgcgagcaag gccgaggtcg tcgcgcatgc gttggcccaa ctcgacccgc
     3841 tgcccgagca cgtgctgatg gtcggcgacc gcagccacga cgtgcacggc gcgtcacacc
     3901 acggtatcga cacggtggtc gtcgaatggg gttacggtgc aggggatttc gaagatccag
     3961 aagtgcagcg cccgcttgcc caggtcccga cggtgtccgc gctgcgtgag gtgctcggtg
     4021 tctgaccacc tgcacgtcac gttcgtgtgc accggcaaca tctgccggtc gcccatggcg
     4081 gagaagatgt tcgcccatca gctgcgcgaa cgaggcctgc acgaacacgt ccgggtgacg
     4141 agcgcgggca ccggtgactg gcacgtcgga tccgaggccg acgcacgcgc gagccttgtc
     4201 ttgcgtgcgc acggctatcc gaccgcgcat tgtgcggcgc agctggacga cgaccacttg
     4261 agcgcggatc tcgttgtcgc gctgggccac aaccccgcgc ggatgcttgc tcatcttggt
     4321 gtgccgggcg aacgggtccg gctgctgcgt tcgttcgacc cgcggtccgg cgcgcacgca
     4381 ctcgatgtcg aggacccgta ctacggcgcg cacgccgact tccaggagac gttcgacgtg
     4441 atcgaggcgt cgctgcccgg cctgcacgac tgggtcgacg agcagctcgc cgcccgcggt
     4501 ctggtcggct gatgcggcgc tgggccttcg tgttccggcc gggctggatt gcgctggcgc
     4561 tggtggtcgc ggctttcgcg tacctgtgct tcacggtgct cgcgccgtgg cagctcggca
     4621 agaacagcaa gacgtctcgc gagaacgacc agatcgcagc gtcgttgacg tccgatccag
     4681 tgccggtgac ttcgcttcta ccgcaggctg attcggcgca gtggcgaagg gtcaccgcga
     4741 ccggtcacta c
//
LOCUS       DASWBF010000059         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_663201,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000059 DASWBF010000000
VERSION     DASWBF010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663201"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            518..1687
                     /locus_tag="VF874_01705"
     CDS             518..1687
                     /locus_tag="VF874_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9174845.1"
                     /translation="MLLRCAAACTTTVAGSAAAPPPAPAAVTIAPAPNARDVDPLAPV
                     SVSAANGKLTEVQMVNDAGKPIEGIMTPDDTSWKPLVPLGYGRTYTITAASRGTGGMV
                     SAQVSKFTTLTPGNQAKVYLDTTSGAALQDGGVYGVGVVVVAHFDEPVADRAAAERRL
                     TVTTSPPVAGSWYWVDDQNAHWRPEHYYVPGTKVTVAANIYGVALGNGLYGQEDAREA
                     FSIGDAHVSVADDATKQVSVYDNGTLVRTMPTSMGMGGTQQIGGRTLSFWTPPGIYTV
                     LDKGNPVVMDSSTFGLPINSRLGYRETIKYATRISTDGIYLHELDSTVWAQGNTDTSH
                     GCLNLNADNAIWFYNFSQPGDVVEVRNTGGPPLQIGQNGDWTVPWDQWRKGSALG"
     gene            1837..>2098
                     /locus_tag="VF874_01710"
     CDS             1837..>2098
                     /locus_tag="VF874_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEX9174846.1"
                     /translation="MTAAGSSYKGLRFPPEIISHGVWLYHRFPLSFRDVQELMLKRGV
                     DVSYETIRAWCDRFGQEYANQLRRRGRRPGDKWHLDEVFVQIN"
BASE COUNT          383 a          700 c          686 g          329 t
ORIGIN      
        1 gcattttcga ccacgaaggc atcggctcag cgcgacccaa tggcgaaccg aaatagatga
       61 gcgcttcgcg gtctggcgtg aggttaccgc gctcgacgct gccgcctgaa ataccaacgc
      121 cccaacgctg atccatcgcc acagctccac aatcaactgc gccactacat atttcaatta
      181 acgtgacagt gcccgcagca ggaccaggcc gacgccgccg agacgagaag gaggcggccg
      241 ccgaaagcgc ccgcgccggc gcaaaggtcg cccacaatcg cgagcgcgca cagcaacgct
      301 aagtcggcga cgtgagcccg tgcccgccgt gtcgggtcac acgcgttcgt gtcacagtga
      361 actgggacgc ggcgacgata ccctcgacgt aggcgtatcc gctgtcgctg atcgctgttg
      421 ggggaaggtg tccgtgagac ataagcacag attccgcgtg ctggccgtgg ccgccgtgat
      481 cgtcatgtcg gcgacctcga tcaccatggc gagcgcgatg ctgctgcgct gcgcggccgc
      541 ctgcacgaca accgtcgctg gcagcgccgc ggcaccccca ccggcacccg cggcggtcac
      601 gatcgccccc gcgccgaacg cccgcgacgt cgacccgctc gccccggtgt cggtgagcgc
      661 cgccaacggc aagctgaccg aggtgcagat ggtcaacgac gccggcaaac cgatcgaggg
      721 catcatgacc cccgatgaca cgtcgtggaa gccgctggtg ccgttgggat atggccgcac
      781 ctacacgatc accgccgcca gccgcggcac cggcgggatg gtgtccgcgc aggtgtcgaa
      841 gttcacgacg ttgacgccgg gcaaccaggc caaggtctat ctggacacca cctccggagc
      901 ggccctgcaa gacggcggcg tctacggggt cggcgtcgtc gtggtcgcgc acttcgatga
      961 gcccgtcgcc gaccgcgccg ccgccgaacg gcggctgacc gtgaccacct ctcccccggt
     1021 ggccggctcg tggtactggg tcgatgacca gaacgcccac tggcgtcccg agcactacta
     1081 tgtgcccggc acgaaagtca ctgtggccgc gaacatttac ggtgtcgcgc tgggcaacgg
     1141 gctctacggg caagaagacg cccgcgaggc gttctccatc ggcgacgccc acgtgtcggt
     1201 cgccgatgac gccaccaagc aggtcagcgt ctacgacaac gggacgctgg tgcgcaccat
     1261 gccgacgtcg atggggatgg gcggcactca gcagatcggc gggcgcacgc tgtcgttttg
     1321 gaccccgccg ggcatttaca cggtgctcga caagggcaac ccggtggtca tggactcctc
     1381 gacgttcggg ctgccgatca attcgcggct cggctaccgc gaaaccatca agtacgccac
     1441 ccgcatcagc accgacggca tctatctgca cgagctcgac agcaccgtgt gggcccaggg
     1501 caacaccgac acctcgcacg gatgcctgaa cctcaacgcc gacaacgcga tctggttcta
     1561 caatttctcc cagcccggcg atgtggtgga ggtccgcaac accggcgggc cgccgctgca
     1621 gatcgggcag aacggcgact ggacggtccc gtgggaccag tggcgcaagg gcagcgcctt
     1681 gggctgacca ccgagggcgg ctatgccccg gcggtcagga gccggttttc agtgcgacgt
     1741 tccggctttt atgttgagct gctattacac cctcacctcg gacggcactg tcaggttggt
     1801 gagccgggaa ggtgaggtgg ggcaggatcg acaatcgtga ccgctgcagg ctcgtcgtat
     1861 aaagggttgc ggtttccgcc cgagatcatc agccatggcg tctggctgta ccaccggttc
     1921 ccgttgagct tccgcgacgt gcaggagctg atgctcaaac gtggtgtgga cgtgtcctat
     1981 gagacgatcc gggcctggtg cgaccggttc gggcaggagt acgcgaacca gctgcgccgt
     2041 cgtggtcggc ggccgggcga caaatggcac ctggatgagg tgttcgtcca gatcaacg
//
LOCUS       DASWBF010000060         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_333104,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000060 DASWBF010000000
VERSION     DASWBF010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333104"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VF874_01715"
     CDS             <1..640
                     /locus_tag="VF874_01715"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9174847.1"
                     /translation="DFVAGLVNHYRRAADEWTGSSELSLALAPNALDTIAQAPDVPVV
                     GTARPFGTKGSTSLGVCELTHRDAVLGVGTVRSVHIAMPGEFSPEPDHPAADGRPTEL
                     AEIMALRVEDGALFQSPNPILNNSMGIVHGGIAAAGLELAASAALNAGRADDPLNTAS
                     LRVNYLRSFRSGSESHYTAEVFRAGRRSGVADAHAIGTDGEVALVARVTAYR"
     gene            662..1033
                     /locus_tag="VF874_01720"
     CDS             662..1033
                     /locus_tag="VF874_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9174848.1"
                     /translation="MSTTREEGLAVLRRLVPDLPFNEDGELPTSDSFAAGLLDESLEC
                     VFGRVWTRPGLDWRSRSLVTLGIMIALRAEGELRLHFPIALRNGLTREELEEVVFHAA
                     AYAGFPAASTAGRIGQEVLDQ"
     gene            1030..1842
                     /locus_tag="VF874_01725"
     CDS             1030..1842
                     /locus_tag="VF874_01725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9174849.1"
                     /translation="MKSLTDKVAIITGAASGIGRATAVSLAHRGARIVAADVNSDGAD
                     AVSAEIAAAGGNAAGVRCDVLDDNAFDDLKVFTLDHFGAVDVVMNNVGGLTRGLPDHL
                     PVREWQRVLDLNLMSVVRSNAAFLPLLIGQGHGHIVNTASFAGLFSYSYDRLPYAAAK
                     AAVVQLSEGLHLYLHPLGIGVTVLSPGPVATNIIASLPPTFGPEVPTRGPGEQFTVLA
                     PEVVGEQVADAIVNDTFMLYTHTGVRDVLVERASDWNAFIARQTDEVAGRAE"
     gene            1855..2724
                     /locus_tag="VF874_01730"
     CDS             1855..2724
                     /locus_tag="VF874_01730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9174850.1"
                     /translation="MQFWSGTAFMDATEAVAVAEMLDEAGYDGIVSSDHLIYPRELKS
                     RYPSPEGRPGWPPETPWPDSWVLIGAMAAVTKRVRFSNAVYVAPARPLLEVAKQVATA
                     SVVSQGRVSLAVGVGWMREEYELLGQDFATRGKRLDEMIPALRALWRGGWVSWSGYYY
                     RVPELMIEPHPVDPVPILCGGESEAALRRAAGLCDGWVGYAYRWDEAIGYVEKLTALR
                     REYGRENEPFEILLALLEPPTPDLYKRAEDAGVTAVMCAPWVGQIDLQPGDPERYRAP
                     IERFAETIIEKAQ"
     gene            2955..3267
                     /locus_tag="VF874_01735"
                     /pseudo
     CDS             2955..3267
                     /locus_tag="VF874_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854413.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
BASE COUNT          529 a         1090 c         1111 g          537 t
ORIGIN      
        1 cgatttcgtc gccggactgg tcaatcacta ccgtcgggcc gccgacgagt ggacggggtc
       61 gagtgagctg tcgctggcgt tggccccgaa cgctctggac actatcgcgc aggcccccga
      121 tgtccccgtg gtcggcaccg cgcgaccgtt cggaaccaag ggctcgacgt cgcttggcgt
      181 gtgcgaactc acccaccgcg atgctgtcct cggcgtcggg accgtccgct cggtccatat
      241 cgcaatgccg ggagagtttt cgccggagcc ggatcacccg gcggccgacg ggcgacccac
      301 cgagctcgcc gagatcatgg cgctacgcgt tgaagacggt gcactgttcc agtcgccgaa
      361 cccgattctc aacaacagca tgggcattgt gcacggcggc atcgcggccg cgggcctgga
      421 gctggcggcc tccgccgcgc tcaatgccgg ccgcgccgac gatccgctga acaccgcgtc
      481 gttgcgagtg aactacctga ggtcattccg cagtggcagc gaatctcatt ataccgcaga
      541 ggttttccga gccggccgtc gcagcggcgt cgcagacgcg cacgcaatcg gcacagacgg
      601 cgaagtcgcc ctcgtcgccc gggtcaccgc ctaccgctga ccgtaagaca aggaggaaaa
      661 ggtgagcact acgcgtgagg aaggcttggc cgtgcttcgg cggctggtgc cagatctgcc
      721 gttcaacgaa gacggcgagt tgccgacgtc ggacagtttc gcggccgggc tcctcgacga
      781 gagcctggaa tgcgtcttcg gacgggtgtg gacccgcccc ggactggatt ggcgctcccg
      841 aagcctggtg acgctgggca tcatgatcgc gttgcgcgcc gaaggcgaac tgcggctgca
      901 ctttccgatc gcattgcgca acggcctgac ccgcgaagaa ctggaagagg tcgtctttca
      961 cgccgccgcc tatgccggct ttccagctgc ttccaccgcc ggtcgcatcg gacaggaagt
     1021 gctcgatcag tgaagtcgct gacggacaag gtcgcgatca tcaccggcgc cgcgagcggt
     1081 atcggccggg cgaccgccgt ttcgttggcc caccgcggtg cccggatcgt cgcggccgac
     1141 gtcaacagcg acggcgccga cgcggtgtcc gcggagatcg ctgccgccgg tggaaacgcg
     1201 gccggtgtcc gctgtgatgt gttggacgac aatgcgtttg acgatctcaa ggtgttcact
     1261 ttggatcact tcggcgccgt ggacgtcgtg atgaacaatg tcggcgggct gacccgcggc
     1321 cttcctgatc accttccggt gcgggaatgg cagcgggttc tcgacctgaa cctgatgtcg
     1381 gtggtgcgca gcaacgcggc gttcctgccg ctgctgatcg ggcagggaca cggccacatc
     1441 gtcaacacgg cttcgttcgc ggggttgttc agctactcct atgaccggct gccctacgca
     1501 gccgccaagg ccgcagtggt ccagctgtcc gaggggctgc atctttacct acaccctctg
     1561 ggcatcggcg tgaccgtgct gtccccggga cccgtcgcca cgaacatcat cgcgtcgctg
     1621 ccgccgacct tcgggccgga agtgccgaca cgcgggcctg gggagcaatt caccgtgctg
     1681 gcacccgagg tggtcggcga acaggtcgcc gacgcgatcg tcaacgacac gttcatgctc
     1741 tacacccaca ccggcgtgcg tgacgtcctc gtcgagcgcg cctcggactg gaacgcgttc
     1801 atcgcccggc agaccgatga ggtcgccggg cgtgctgaat gaaaggcgcg aaaagtgcag
     1861 ttttggtctg gcaccgcgtt catggacgcg acggaagccg ttgcggtggc cgagatgctc
     1921 gacgaggccg gctacgacgg catcgtcagc tccgatcacc tcatctaccc gcgcgagctg
     1981 aagtcgcggt atccgtcgcc ggaaggccgg ccgggctggc ctcccgaaac gccgtggccg
     2041 gactcgtggg tgctgatcgg ggcgatggcg gccgtgacga agcgggtgcg gttctccaac
     2101 gcggtgtacg tcgcacctgc ccgcccgctg ctggaggtgg ccaagcaggt ggcgacggcg
     2161 tcggtggtgt cgcagggccg ggtgtcgctg gcggtcggag tcggctggat gcgcgaggaa
     2221 tacgagttgc tgggccaaga tttcgccacc cgaggcaagc gtctggatga gatgatccct
     2281 gcgctgcgcg cgctgtggcg tggcggctgg gtgtcgtgga gcggctacta ctaccgagtg
     2341 cccgagctga tgatcgagcc gcatcccgtc gaccccgtgc cgattctgtg cggcggcgaa
     2401 tccgaggccg cgctgcgccg cgcggcaggc ctgtgcgacg gctgggtggg ctacgcgtac
     2461 cggtgggacg aagcaatcgg ctacgtggag aagctcacgg cgctgcgccg tgaatacggc
     2521 cgggagaacg agccgttcga gatcctgctg gcactgctcg agccgcccac cccggacctg
     2581 tacaagcggg ccgaagacgc cggggtcacc gcggtgatgt gcgctccatg ggtcggccag
     2641 atcgacctcc agccgggcga cccggaacgc taccgggcgc ccatcgagcg gttcgccgag
     2701 accatcattg agaaggcgca gtagtccgac gtgcgcgccc gtttcctcgg ctagccgcgt
     2761 cccccaatcg gacgacatcc gattcgtccg gtgcacggcc tgaacgccgg acagacgccg
     2821 gccacctgcg cggcaatagt gacctcatgc ctcaagcact gcgaatcacc agccggacga
     2881 gcgacgcggc tccgctcccc gcccgtcggc tcgcgaaggt gatcgcactt cgacccggca
     2941 gcggacgcca cgtcatcaag catcgtcctc accagcggac cctcgctcag tccgcgaagc
     3001 tgcacgacgt cctctacgag atccgggggc cgctgcacga ccacgcggca cggctggagg
     3061 cgcaaggcca ccgcatcctg aagctgaaca tcggcaaccg gcgccgttcg ggttcgacgc
     3121 gcccaccgag atcgtccgcg acatgattca gacgctgccg accgcgcagg gctactccga
     3181 ctcgaaggga gttcttccgg cgcgccgcgc ggtggtgacg cgctacgagc tcgtcgaggg
     3241 tttcccgcgc ttcgacgtcg acgacgt
//
LOCUS       DASWBF010000061         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1190119,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000061 DASWBF010000000
VERSION     DASWBF010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190119"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VF874_01740"
     CDS             <1..421
                     /locus_tag="VF874_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174851.1"
                     /translation="AAATVERIRPRRAICSSPAGCGRSHVLLPRFCLGRRGGGDLGGA
                     DGPGRRLGVAADHRGGGSSGLDGAGLAVGFDAHAEPVRVGFARLKRHVNAGADMDRLA
                     PAASRVADAVAQIGSACAAVRRGNRWWAARVGRQRLR"
     gene            complement(597..1611)
                     /locus_tag="VF874_01745"
                     /pseudo
     CDS             complement(597..1611)
                     /locus_tag="VF874_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293166.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
     gene            1726..>2763
                     /locus_tag="VF874_01750"
     CDS             1726..>2763
                     /locus_tag="VF874_01750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9174852.1"
                     /translation="MEGTPFGRYRLIELLGRGGMGEVWRAYDTAIDRVVALKVLPANL
                     SDDEVFQERFRREARAAGRLQEPHVVPIFDVGEIEGRLYVTMRLIEGRDLQSMLAEGP
                     LRPTRAVWIIEQIASALHAAHRIGLVHRDVKPSNILIVEDDFAYLIDFGIARAGGETG
                     LTSTGATIGTWAYMAPERFTTGQIDPRSDIYALACVLHECLTGSRPFAGDSLEQQVAG
                     HLMTPPPQPSALRDGIPGELDAVIATGMAKNPDERYPTTKDLGRAAHAAIITSPPIAP
                     PAQPSQRATEFTPAPSREGFASKPILADLNAVGPTSLSAAAPTEPRPVTMPTHSRLLA
                     PFGGSTSDTPRD"
BASE COUNT          446 a          839 c          992 g          486 t
ORIGIN      
        1 ggccgcggcg acggtggagc ggatccggcc gcggcgggcg atctgctcaa gcccggcggg
       61 ctgtggccgc tcgcatgtgc tgctaccccg gttctgcctg ggccgacggg gtggcggtga
      121 tctgggcggc gctgatggcc cgggccgccg gctgggggtg gcggcggatc accgcggcgg
      181 cgggtcgtcc ggcctcgacg gtgcggggct ggctgtcggg ttcgacgcgc atgccgagcc
      241 ggtccgggtc gggttcgcgc ggctgaaacg ccacgtgaac gccggcgccg acatggatcg
      301 tctggccccg gccgcaagcc gggtcgccga cgcggtcgcg cagatcgggt ccgcgtgcgc
      361 ggcggtgcgc cgcggtaacc gttggtgggc tgcccgcgtt ggtcgacagc ggctgcggtg
      421 atggcgtccg tccctgcagg ttcagcggtg gttgttgcag gcgctggagg aacgggcggg
      481 gcagcgacgc tacctgtcgg gacgcccggt ggagatgtct gtgcgggcga tgtgaggttt
      541 gacggctgca ccgtgtcgga ttggccgtct ttccagggtt gccatcacag cgcgatgacg
      601 ccgatcaaga cgatcaccgc gagcgcggct cctgggatca gccactgctt tcgcgggcca
      661 ttgatccggt gagaaaagct cggtgctcgg tcagttgccg caggagcggt tcgcttccgc
      721 gcagcgggcg cgggtgccgt aggtgcggta ggcgatgcgg cccggggcgt cgcgtcttga
      781 cgctcagtga gttcgcgggc gaagtctgcg caccgccgaa atcgatcttc gggatttttg
      841 gctagcgccg ccgcgaagac tgggtcgagg gcagcaaggt cgggcaggat gtctgccagg
      901 gccgggggca gtgagttgag gtgtcgactg atgacgaccg ctggattgga atgcgggaat
      961 agctgtgaac ccgtcaagag gtgataggcg gtagcggcca gcacgtattg gtcggtgcga
     1021 ccatcgatat cttcgcccat cagttgctcg ggtgcggcat acgcgacagt gccaacggtc
     1081 atgttggtag tggtgaggcc gctgatgtcc tcgatgtcgc gggcaatccc gaagtcggcc
     1141 aacaagattc gttgctcacc gtcgtcgaga ggggtgagca tgatgttcgc cggtttgacg
     1201 tcgcggtgca gcaggccctg cttatggcca tagtcgagcg cgctggcgac agcggtgacg
     1261 atccgcacga cttgatcgac tggcattccg gcggggaacc gctcagtgag gaggcggctc
     1321 gcgtcttgac cgtcgacaaa gtccatcgag agccacagct ggccgttgtc ttcgccccgg
     1381 tcgtgcacgc tgacgatgtg cggatgccaa agcgtggacg ccaggtcggc ttcgcggttg
     1441 aatcgggcgc ggtactcagg atcagccgac acgtcggcgg gaagaatttt tagaccatcc
     1501 cgacgaggca gcctcggggt gctcggccag gtaaacctcg cccatcccgc ctgaaccgag
     1561 cagacgcatg atcctgtatc ccgcgaacgt gctaccgcca gctaacggca tgggcggaca
     1621 ttaccgaacg tcgcagggag ccggtcatag cacttttcac cttctcccac cttggctgtt
     1681 tgaccggctt atggtgtctc tgaccgcgca tgccgggagg ctgatgtgga gggcacgccg
     1741 ttcggacgct accgcctgat tgagttgctg ggccgtggcg ggatgggaga agtgtggcgg
     1801 gcctacgaca ccgcgatcga ccgggtggtg gcgctgaagg tgctgcccgc aaacctcagt
     1861 gacgatgagg tatttcagga gcggttccgc cgggaggcgc gggcggcggg ccggttgcag
     1921 gaaccgcatg tggtacccat tttcgacgtc ggggagatcg aggggcggct gtatgtgacc
     1981 atgcggctga tcgagggccg cgacctgcag tcgatgctgg ccgaggggcc gctgcggcca
     2041 acgcgggcgg tgtggattat cgagcagatc gcctcggcgt tgcatgccgc gcaccggatc
     2101 gggttggtgc accgcgacgt gaagccgtcc aacatcttga tcgtcgaaga cgatttcgcg
     2161 tatctgatcg acttcgggat cgcgcgtgct ggcggagaaa ccggactaac gagcactggc
     2221 gcgacgatcg gcacctgggc gtacatggcc ccggagcggt tcaccaccgg ccagatcgac
     2281 ccgcgctccg acatctacgc gctggcctgc gtgctgcacg agtgtctgac cggcagtcgg
     2341 ccctttgccg gggacagcct tgagcagcag gttgccggac atttgatgac tccaccaccg
     2401 caaccgtcgg cactgcggga cggcataccc ggcgaactcg acgccgtcat cgccaccggt
     2461 atggccaaaa acccggacga gcggtacccg acgaccaaag atttgggccg agcagcgcat
     2521 gccgcaatca ttacctcacc gcctatagcg ccaccggctc aaccgtcaca acgagccacg
     2581 gagttcacgc cagcacccag ccgcgagggt ttcgcctcca agccaatcct cgcggacctt
     2641 aacgccgtag gaccgacatc gttgtctgct gccgcgccga ctgagccgcg tccggtgaca
     2701 atgccgacgc actcccggct gctggcacct ttcggtggat caactagcga cacaccccga
     2761 gac
//
LOCUS       DASWBF010000062         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_926265,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000062 DASWBF010000000
VERSION     DASWBF010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926265"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF874_01755"
     CDS             <1..214
                     /locus_tag="VF874_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174853.1"
                     /translation="VRHLLGYRLSIAELIGIAFLVGFPYLLVGVLWSINHTDHLHEMH
                     GADLMFSFVGAIVFWPVLLIADVCMV"
     gene            234..587
                     /locus_tag="VF874_01760"
     CDS             234..587
                     /locus_tag="VF874_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174854.1"
                     /translation="MTNRGWLIFCVGVALLGVGLLALNFPVFIDAWDQWGWQIKCGTG
                     YSTDLMQAEIATQAAPHTNLVDQCQSALAVRRAWSGTAALLGWVVLSGLAVTLWRHAS
                     AHHRESERENESVNA"
     gene            complement(584..982)
                     /locus_tag="VF874_01765"
     CDS             complement(584..982)
                     /locus_tag="VF874_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9174855.1"
                     /translation="MIYLDTSALVKLVRREQESDGLADWLDEQGSIPWVSSALIEVEL
                     PRALRRSDPRLIADAPALVARVARYEIDDVVRAAAAAYPDPDLRSLDAIHLATAKAVF
                     GNSLTAFVAYDERLLAAAADQGLAVERPGR"
     gene            complement(982..1254)
                     /locus_tag="VF874_01770"
     CDS             complement(982..1254)
                     /locus_tag="VF874_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9174856.1"
                     /translation="MADVPVRELNQDTAGVLARVKQGERVNITERGQVIASIIPARDN
                     PLSDLISSGKLHPATISGPAPRPGGPIKTDHEAGELLREMRDSERY"
     gene            1307..2512
                     /locus_tag="VF874_01775"
     CDS             1307..2512
                     /locus_tag="VF874_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9174857.1"
                     /translation="MTGPPLERRLGLTDAVVIGLGSMIGAGVFVALAPAADAAGSGLL
                     VGLAVAAVIAYCNATSSARLAARYPQSGGTYVYGRERLGPFWGYLAGWGFLVGKTASC
                     GAMALTVGYYVWPAQAHLIAVATVVVLTTINYVGVQKSALTTRLIVAVVFMVLAVVVI
                     VVFTSGAAYFSRLQLGGRPGGVLRAAGLLFFAFAGYARIATLGEEVRDPTRTIPRAIP
                     LALGITLAVYATVAVAALSVLGSDGLARATAPLADAVRAAGMPGFEPVVRAGAAVAAV
                     GSLLALLLGVSRTTLAMARDRFLPQGLSAVHPKFKVPHRAELVVGTVAAVVAAFADIR
                     GAIGFSSFAVLVYYAVAHASAWTLGHRILPTVGLVGCLALAFALPLTSVLSGAAVLVL
                     GAAIYTVRR"
     gene            complement(2494..>2782)
                     /locus_tag="VF874_01780"
     CDS             complement(2494..>2782)
                     /locus_tag="VF874_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HEX9174858.1"
                     /translation="FRKRALRAVHMNPAPVEWFVLNAEANVEVDLTALDALDDLRAEL
                     TSRGIVFAMARVKQDLLSALQAAGLLEKIGEDRIFMTLPTAVEAFNQRRTV"
BASE COUNT          399 a          898 c          924 g          561 t
ORIGIN      
        1 ggttcgccac ctactcggct accgtctgtc tatcgcggag ctgatcggta tcgcgttcct
       61 tgtcggattt ccctacctgc tcgtcggagt gctctggtcg atcaaccaca ccgaccatct
      121 gcacgagatg cacggcgccg atttgatgtt ctccttcgtg ggagcaatcg tgttctggcc
      181 cgtgctgctc atcgccgacg tctgcatggt ctgacccgcc caaggaggcc cctgtgacga
      241 accgtggttg gctcatcttc tgtgtcggcg ttgcgctcct tggtgtcggt ctgctggcac
      301 tgaacttccc ggtgttcatc gatgcgtggg accagtgggg ctggcagatc aagtgcggca
      361 ccggatacag caccgatctc atgcaagccg aaatcgccac ccaagcggcg cctcacacga
      421 acctcgtcga ccagtgccaa agtgcgctgg cggtgcgccg cgcatggagc ggaacggccg
      481 cgcttcttgg ctgggtcgtg ctgtctggcc tcgccgtaac gctgtggcgg cacgcgtcag
      541 cccaccatcg cgaaagcgaa cgtgaaaacg aaagcgtcaa cgcctagcga cctggacgtt
      601 caaccgcaag gccctggtcc gcggcggcag ccagcaggcg ctcgtcgtag gcgacgaacg
      661 cggtgagtga gttcccgaaa acggccttcg ccgtcgccag atgaatcgcg tccaaggatc
      721 gaagatccgg atccggatac gccgctgccg ctgcccggac gacgtcgtcg atctcgtagc
      781 gagcgacgcg ggcaacaagt gcgggcgcgt ccgcgatgag ccgcggatcg gatcgacgca
      841 acgctcgggg aagttccacc tcgatcagcg ctgacgagac ccacgggatt gatccttgct
      901 catcgagcca gtctgcgagt ccgtcgcttt cctgctctcg ccgaacgagt ttcacaaggg
      961 ccgacgtgtc aaggtaaatc atcagtaacg ctcggaatca cgcatttcgc ggagcaactc
     1021 ccccgcctcg tgatccgtct tgattggccc gccggggcgc ggcgccggcc cagagatcgt
     1081 ggccgggtgg agttttcctg aactgatcag gtccgacaac gggttgtccc gtgccggaat
     1141 gatgctggcg atcacctggc cgcgttcggt gatgttgacg cgctcaccct gcttgacgcg
     1201 ggccaggaca ccggcggtgt cctggttgag ctcgcgtacg gggacgtcgg ccatattccg
     1261 agcgtactac gttattgtac tacatgtctt cacttaacct aaacccgtga ccggaccgcc
     1321 cctcgagcgt cgtctgggtc tcaccgacgc ggtcgtcatc ggtctgggtt cgatgatcgg
     1381 ggcgggcgtg ttcgtggcgc tggcgccggc ggccgacgcc gcgggttcgg gactactcgt
     1441 cggtctggcc gtcgcagccg tcatcgcgta ctgcaacgcg acgtcgtcgg cgcgactggc
     1501 cgcgcgctac ccccagtccg gcggcaccta cgtgtacggc cgcgaacggc tcggcccgtt
     1561 ctggggatac ctggccggct ggggcttcct ggtcggcaag accgcgtcat gcggggcgat
     1621 ggcgctgacc gttggatact acgtctggcc ggcgcaagcg catctgatcg ctgtcgcgac
     1681 cgttgtcgtg ttgacgacga tcaactatgt cggcgtccag aagtcggcgc tcaccacgcg
     1741 actgatcgtc gccgtcgtgt tcatggtgtt ggcggttgtc gtgatcgtcg ttttcacttc
     1801 cggcgcagca tatttcagcc ggttgcagct cggcgggcgg ccaggcgggg tgctgcgggc
     1861 cgccgggctg ctgttcttcg cgttcgccgg atatgcccgc atcgccacgc tcggtgagga
     1921 agtccgcgat ccgacgcgca ccattccccg cgcgatcccg ttggcgctgg gtatcacgct
     1981 ggccgtctac gcgacggtgg ctgtcgccgc gttgtcggtg ctcggcagtg acggactggc
     2041 ccgcgcgacc gcgccgctgg cggatgccgt cagagccgcc ggaatgcccg gcttcgagcc
     2101 ggtggtgcgg gccggcgccg cagtggccgc ggtggggtcg ctgctggcct tgctgctcgg
     2161 ggtatcccgc acgacactcg cgatggcgcg cgatcgcttt ctgccacaag gtctttcggc
     2221 tgtacatccg aagttcaagg taccgcaccg cgcggagctg gtcgtcggca ccgtggccgc
     2281 ggtcgtggcg gccttcgccg acatccgcgg tgcgatcggg ttctcctcgt tcgcggtgct
     2341 ggtgtactac gcggtcgccc atgcgtcggc gtggacgctg ggacatcgga tccttccgac
     2401 ggtcgggctg gtcggctgtc ttgcgctggc gttcgcgctg ccgctgacat cggtgttgtc
     2461 gggtgctgca gtgttggtgc tcggtgcggc gatctacacc gtgcgccgtt gattgaacgc
     2521 ctcgaccgcc gtgggcagtg tcatgaagat ccggtcttca ccgatcttct cgagcagccc
     2581 cgcggcctgc agcgcactca gcagatcttg cttgactcgt gccatcgcga aaacgattcc
     2641 tcgactggtc aattcggccc tgaggtcatc gagcgcgtcc agcgcggtga ggtcgacctc
     2701 gacgttggct tctgcgttga gcacgaacca ctccaccggc gccgggttca tgtggaccgc
     2761 tcgcagtgcg cgcttgcgga ag
//
LOCUS       DASWBF010000063         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_401053,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000063 DASWBF010000000
VERSION     DASWBF010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401053"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /gene="mftF"
                     /locus_tag="VF874_01785"
     CDS             <1..955
                     /gene="mftF"
                     /locus_tag="VF874_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778565.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HEX9174859.1"
                     /translation="CTTDFVAFLDSDVVPRRGWLEALLGHFSDPAVALVAPRIVGLSH
                     GDNMIARYEAVRSSLDLGRREAPVVPYGTVSYVPSAAIICRRSALVAAAGFDETLRCG
                     EDVDLCWRLVESGARLRYEPIAMVAHDHRVGVRDWLARKAFYGESAAPLSIRHPDKTA
                     PLVISGWTLLLWLSMAMGSCLGYLVSAVIAALAARRIANTLDGVDSNPRDVAMLAARG
                     LGSAALQLASAICRHYWPIALVAALLFRRFRHIVLVAAVVDGVADWVSRHNNDDAQTK
                     QLGLIPYLVLKRLDDLAYGAGMWVGMVQERHLGPLKPQIRT"
     gene            962..2398
                     /gene="mftG"
                     /locus_tag="VF874_01790"
     CDS             962..2398
                     /gene="mftG"
                     /locus_tag="VF874_01790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system GMC family oxidoreductase
                     MftG"
                     /protein_id="HEX9174860.1"
                     /translation="MHSDVLIIGAGSAGCVVAERLSANERCVVTVVEAGPAVVDPSIR
                     AMVADGWVLPIGAVSPVVRRFETVLTDRPRRVTNVVRGEVVGGSGAVNGGYFCRGLPR
                     DFDGWGIPGWSWSDVLPHFRDIETDLDFDGPAHGQRGPIPVRRTSELSRNTQDFIDRA
                     CDAGFDWIPDLNGAVDPVTNVAGVGAVPLNFTNGTRVQPGSAMLEPALRRPNLTLLTG
                     TSVWRLRVSSGRVTSADAEGPSGPVTLSAERIILCAGAIGTARILMLSGIGDEAVLRA
                     AGIRLVQPAPVGMNCVDHPEWVMPTTWTTSPGRPVLEAILATDAGVEIRPYTGGFVAM
                     MGDGTAGRPDWPHVGAALMQPRSRGRVTLASDDPRVPPRIEHRYDSEPADVVALRDGA
                     ELARQLCPVGQPALEPLWSTSQHLCATAPMGSDRDERAVLDPRCRVRGVDGLWVIDGA
                     ALPAITSRGPHATIVMFAHRAAEFVVAE"
     gene            complement(2485..2684)
                     /locus_tag="VF874_01795"
                     /pseudo
     CDS             complement(2485..2684)
                     /locus_tag="VF874_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          394 a          899 c          987 g          473 t
ORIGIN      
        1 ctgcaccacc gacttcgttg cgtttctgga ctccgacgtc gtgccgaggc gcggctggct
       61 cgaagcgctg ctgggtcatt tctccgatcc cgcggtggcc ctcgtcgcgc cgcggatcgt
      121 agggctttcc cacggcgaca atatgattgc gcgatacgag gccgtccgat cgtcactgga
      181 tctcgggcgg cgggaggcgc cggtcgtccc gtacggcacc gtgtcctatg tgccgagcgc
      241 ggcgatcatc tgccgtcggt cggctctggt tgccgcggcc gggttcgacg agacgttacg
      301 ctgcggcgag gacgtcgacc tttgctggcg cctggtggag tcgggtgccc gcctgcgtta
      361 cgagccgatc gcgatggtcg cgcacgatca ccgcgtcggt gttagagact ggctggcccg
      421 caaggcgttc tatggcgaat ctgcggcacc gttgtcgatc cgccatcctg acaagacggc
      481 cccgctggtg atctccgggt ggacgctgct gctgtggctg tcgatggcga tggggtcgtg
      541 tctgggatac ctggtgtcgg cggtgatcgc ggcgctggcc gcgcggcgga tcgccaacac
      601 gttggacggc gtcgactcga accctcgtga cgtggccatg ctggccgcgc gggggctggg
      661 ctcggcggcg ctgcaattgg cgtcggcgat ctgccgccac tactggccga tcgcgctggt
      721 cgcagcatta ttgttccggc ggttccggca catcgtgctg gtggccgcgg tcgtcgacgg
      781 cgtcgcggac tgggtgagca gacacaacaa cgacgatgcg cagaccaagc agctgggact
      841 gatcccgtat ctggtgctca agcgcctcga cgatctcgcc tatggcgccg gcatgtgggt
      901 gggaatggtg caggagcgcc atctcgggcc gttgaagccg caaatccgaa cataacatcc
      961 gttgcacagc gatgtcctga tcatcggcgc cggtagcgcc ggatgcgttg tcgccgagcg
     1021 gttgtccgct aacgagcggt gcgttgtcac cgtggtcgaa gccgggcccg cggtggtcga
     1081 cccgtccatt cgcgcgatgg tggccgacgg gtgggtgctg ccgatcgggg cggtgagtcc
     1141 cgtggtgcgg cggttcgaga cggtgctgac cgaccggccc cggcgagtga ccaatgtcgt
     1201 ccgcggcgag gtggtcggcg gttccggggc tgtcaacggc ggctacttct gccgcggtct
     1261 accgcgcgac ttcgacggct ggggcatacc gggctggtcc tggtcggacg tgctgccgca
     1321 cttccgcgac atcgagacag acctcgactt cgacggtcct gcgcacggtc agcgtggacc
     1381 gatccccgtg cgccggacca gcgaactatc aagaaataca caggatttca ttgaccgtgc
     1441 ctgcgatgcc ggctttgact ggatacctga tctcaacggc gcggtggacc cggtgaccaa
     1501 cgtcgccgga gtcggggcgg tgccgctgaa cttcacgaac gggacgcggg tgcaacccgg
     1561 ctcggccatg ctggagccgg cactgcgacg gcccaacctg acgctgctca ccggcacgtc
     1621 ggtgtggcgg ctgcgggttt cgtccggccg ggtgacttca gccgacgccg aaggaccgag
     1681 cgggccggtg acgctgtcgg cggagcgaat catattgtgc gcgggcgcaa tcgggaccgc
     1741 gcggatcctg atgctgtcgg gcatcgggga tgaggccgtg ctgcgggcgg ccggaatacg
     1801 gctggttcag cccgcgccgg taggcatgaa ctgtgtcgac cacccggagt gggtgatgcc
     1861 gaccacctgg acgacttctc ccgggcgacc cgtcctcgag gcgatcctgg ctacggacgc
     1921 cggtgtcgaa atcaggcctt acacaggagg tttcgttgca atgatgggcg acggaaccgc
     1981 cgggcgaccg gactggccgc acgtcggcgc ggcgttgatg cagccgcgct cccgcgggcg
     2041 ggtcacgttg gcttccgatg acccgcgcgt gcccccgcgc atcgaacacc gctacgacag
     2101 cgagccggcg gacgtggtgg cgctacgtga cggggccgag ttggcccggc agctgtgccc
     2161 cgtcggccag cccgccctcg aaccgttatg gtcgacctcg cagcacctct gcgcgacagc
     2221 cccgatgggt tccgaccgtg acgagcgggc ggtgctcgac ccgcggtgcc gggtgcgcgg
     2281 tgtcgacggc ctgtgggtga tcgacggcgc cgcgctgccg gcgatcacca gccggggtcc
     2341 gcacgccacg atcgtgatgt tcgcccaccg cgccgccgag ttcgtcgtcg cggaatgacg
     2401 gccgcaaccg cgcaccgagc cagcgcacca cccgcagctg ctcgccgtcg cgccccggcg
     2461 cccacaggcc gacgagcaca gcgcgtccac cacccaggtc aattggattt gggtggccga
     2521 gtggccacag cgatgtcgcc cagcgcggcg ttcaacgcga tcatcgatgc gatcaccagg
     2581 cccacacccg cgcacgcgac gacgagtgtc caggtgcggc gccgttgcga cgcgtcggtc
     2641 acatccgggg tggcctggtc ggagttgatg agcgtgtcga ccacagtcgt cagaccagct
     2701 atggagatgt ccttcctgtt gagcgcgcgc cggcatcacg agactatccg tcg
//
LOCUS       DASWBF010000064        13126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_333540,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000064 DASWBF010000000
VERSION     DASWBF010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13126
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333540"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF874_01800"
     CDS             <1..447
                     /locus_tag="VF874_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174861.1"
                     /translation="DQRAAERSYGIAEDDLTPCMSWSFVSLRNDWNQRKHTVAVRADG
                     TPRFRCRRDRRPRSPGHGRARSGVSAQCDKASKLCSGCGDLKAAVNTARYAQRETDPS
                     TGVVTGGADRKPPPTGGAGGRETRTRAEVGDNADDGGSASPKGEAA"
     gene            491..2827
                     /locus_tag="VF874_01805"
     CDS             491..2827
                     /locus_tag="VF874_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9174862.1"
                     /translation="MASFGRDLLASALAGTDPNEHPLRHVADLPAHQGSSAAWPAWAD
                     GDVVSAFVAQGVTTPWSHQVRAATLAQQGRHVVVSTGTASGKSLAYQLPIMCALATDR
                     RARALYLSPTKALGHDQLRAAASLTGAVDSLADVAPASYDGDSPAEVRRFAREQSRWV
                     FSNPDMIHLSMLRNHARWAVFLRNLRFVVVDECHYYRGIFGSNVAMVLRRLLRLCGRY
                     TAAPTVVFASATTASPGATASELIGQTVEEVTDDESPHGARTVALWEPALRTDVTGEH
                     GAPVRRSAGAEAARVMADLVVEGARTLTFVRSRRGAELTALGARARLADAVPALVERV
                     ASYRAGYLAEDRRALERALASGELVGLATTNALELGVDIAGLDAVVLAGFPGSVTSFW
                     QQAGRSGRRGQSALVVLIARDDPLDTYLVHHPEALLDKPIEMTVIDPTNPYVLGPQLL
                     CAATELPLTDAEVQGWGAVEVARALVDDGLLRRRGSGYFAAPGVDPHPAVNIRGSSGD
                     QIAILEADTGRLLGGVEAGRAALSVHPGAVYLHQGETYVVDTLDFDEGVAFVRVDDPG
                     YATYAREVTDIAVTGPGERSTFGDVVLGLVPVAVTNQVIGYLRRTPAGEVIDFVELDM
                     PARTLETVAVMYTITPEALEHNGIDWSQVPGALHAAEHAAIGLLPLVASCDRGDIGGL
                     STAVGPDTGLPTVFVYDGYPGGAGFAERGFRQAATWLSATADAIDACECPQGCPSCVQ
                     SPKCGNGNDPLDKHAAVRVLRLVLSELPAHEVPRPIRR"
     gene            complement(3096..3506)
                     /locus_tag="VF874_01810"
     CDS             complement(3096..3506)
                     /locus_tag="VF874_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEX9174863.1"
                     /translation="MRLCCPASRSQPRRCRPRSRPRSDRGSASIVAASMMAVLLAVTV
                     GVVQLGSAVIARHQAQAAADMAALAAAGVMPAGLTAACDKAAAVARAMSTAVTGCTAD
                     ALDVVVTVDAHVPLAAWDIGAARAVARAGPVTSS"
     gene            complement(3436..3783)
                     /locus_tag="VF874_01815"
     CDS             complement(3436..3783)
                     /locus_tag="VF874_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HEX9174864.1"
                     /translation="MEHQRLADDDGGVTVEAAFAIAALVVVLLLCIAGVTAVSAQVRC
                     IDAAREAARLVARGDDARAEAAARRIAPHDAVLALRHDGDFVVARVTAHSALLPGLSI
                     TAEAVSATEPPAQ"
     gene            complement(3765..3965)
                     /locus_tag="VF874_01820"
     CDS             complement(3765..3965)
                     /locus_tag="VF874_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEX9174865.1"
                     /translation="MTTRWQHLRARMTVLWAAEDGMSTVEYAIGTIAAAAFGAILYTV
                     VTGDSIVNALTNIISRALNTNV"
     gene            complement(3992..4564)
                     /locus_tag="VF874_01825"
     CDS             complement(3992..4564)
                     /locus_tag="VF874_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9174866.1"
                     /translation="MTLAAVLLAAAVLISPGPTNVRRRAGLATRAPARRPTGTKPDPL
                     AVAASLDVLAVCLTAGMAVASAAAATAPASPPRLAALLRRAADMLALGADPAAAWTTP
                     ASGDRHCESLLRLARRSASSGSALAEGVAELAVSVRHEAADAARATAERASVLIAGPL
                     GLCYLPAFVCLGIVPVVAGLAGQVLGSGPW"
     gene            complement(4561..5364)
                     /locus_tag="VF874_01830"
     CDS             complement(4561..5364)
                     /locus_tag="VF874_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9174867.1"
                     /translation="MTGLPAASLALAAALLVVPWSSRHRLVAAGLITRRRSMMPSRAV
                     GVVVCAAGVVTAAALPASIVIAAAILGTTLLLRRRSLAARRRIEQESRALETALDVLA
                     GELRAGAHPVSAFQIAAAESDGHVGESFRAVAARAVLGADVPAGLLSEAGAAARPVYW
                     ERLAVCWELGQTHGLTMATLMRAAQRDIVERARFDAQVIAGMAGARATASVLAGLPLA
                     GVALGGLLGAHPVRFLVSGGAGGWLLVIGVSLVCCGLLWSDRITGQALA"
     gene            complement(5361..6527)
                     /locus_tag="VF874_01835"
     CDS             complement(5361..6527)
                     /locus_tag="VF874_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HEX9174868.1"
                     /translation="MNPSLIDRVRERLAAESAPLRPNVVAAAIRAESGGVLGDTEVLT
                     HLRVLQTELTGAGILEALLCADGTTDVLVTGPDAVWVDDGNGLRRTDIHFTDEAAVRR
                     LAQRLALSAGRRLDDAQPWIDGHLSGLGTGQFAVRLHAILPPLAAGGTCLSLRVLRPA
                     TQDLPALVQAGTIARGAAELVDRIIAARLAFLVTGGTGAGKTTLLAATLGAVSADERI
                     VCVEDAAELAPRHPHLVNLVARAANVEGVGEVTVRDLVKQALRMRPDRIVVGEVRGAE
                     VVDLLAALNTGHDGGAGTVHANRPSEVPARLEALAALGGLDRAAVHSQLAAAVQVVLH
                     VRRGQNGGRRLDEIAVLRRDGDEPVRVVTAWHADAAPAPGADLLRDLLSGRGVR"
     gene            complement(6524..7588)
                     /gene="ssd"
                     /locus_tag="VF874_01840"
     CDS             complement(6524..7588)
                     /gene="ssd"
                     /locus_tag="VF874_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein Ssd"
                     /protein_id="HEX9174869.1"
                     /translation="MRPTSGVLGVVDDPALRDGVDRVAAAVGVRVVYCPASRAASRRE
                     WSAATAVVLDEAAARRGAASGMPRRPRVILLVVGEPQQETWQAAISVGAEHVLRLPDE
                     EGELVAVLSDAGDFDQGARGSAVAVISGRGGGGASIFSAALAIAAADALLVDVDPWSG
                     GVDLLLGLEGEPGLRWPDVAVENGRLSLAAVRDALPCHHRVAVLSGARHGSDITGGSL
                     LAIVDAGRRGGVTVVCDLPRRFTEAAEAALDAAELVVLVAACDVRSCAAASAMAAALI
                     GINPNVGLVVRGPSPGGLRAADVARAAGLPLLAAMRPEPNLAERLERVGLRLRRRSPL
                     LTTARRVLGVLGRQVALEAA"
     gene            7996..8865
                     /locus_tag="VF874_01845"
     CDS             7996..8865
                     /locus_tag="VF874_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX9174870.1"
                     /translation="MTASDQAAAPLTTTQVTPSADCDPESPTKTAAFFDLDKTVIAKS
                     STLAFSKPFFDQGLINRRTVLKSSYAQFLFLMSGADHDQMDRMRSYLTNMCTGWNVEQ
                     VKSIVAETLHDIVNPLVFAEAADLIADHKLCGRDVVVVSASGEEIVAPIARALGATHA
                     MATRMVVEDGKYTGEVAFYCYGEGKAQAIRELADREGYPLEHCYAYSDSITDLPMLET
                     VGHPAVVNPDRGLRKEAASRGWPVLTFSKPVSLRDRIPAPSTAAVATTAAVGVSAVAA
                     GALTYSLLRRFVL"
     gene            complement(9309..10052)
                     /locus_tag="VF874_01850"
     CDS             complement(9309..10052)
                     /locus_tag="VF874_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX9174871.1"
                     /translation="MTDPLAPLADLPGVADAGERVRDALGKVHRHRVNLRGWPATAAE
                     AAVRAAQASSILDGGAPRFPADGLDSGPILAGALRVAEALEGGQTSLVSVWQRAPLQA
                     LARLHALAAADLVDDAALGRPREGVAPRLELLAGLMTGGSSAPAPVLAAAAHGEILTL
                     RPFGSANGVVARAVSRLVTQASGLDPHGLAVPEVYWMRHASEYRAAAQQFATGSADGL
                     RQWMLLCCRALEEGAGEAWSMAEAASKRT"
     gene            complement(10058..11683)
                     /locus_tag="VF874_01855"
     CDS             complement(10058..11683)
                     /locus_tag="VF874_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9174872.1"
                     /translation="MTEPLLSIDGLQVCFGTDLPAVRNAHLAVYPGQTVALVGESGSG
                     KSTTAAAVLGLLAPGGRVTQGRIVFDGHDITHAGRRRLETIRGRQIGYVPQDPMTNLN
                     PVWTIGFQIRETLRANGIADRRAAPKRAVELLDDVGMTDSAMRAGSYPHQLSGGMCQR
                     ALIAIGLAGQPRLLIADEPTSALDVTVQKQVLDHLDELAGQRGTAVLLITHDLALAAE
                     RAEHLVVMYRGTVVESGPAQAIIDDPQHEYTQRLVASAPSLSMRRGAAVRRRVVTEAA
                     AHAGQPIDDMLVLSNVTKVYRVGAPWRQRDVTAVDDVSFRVRRATTTAVVGESGSGKT
                     TVARMVLALLAPTSGTVTFDGRDVATLNGAAAKAFRRRVQPVFQNPYASLDPMYSVYR
                     TIEEPLRTHGIGSRVDRQRAVRDLLDHVALPSSVLRRFPRELSGGQRQRVAIARALAL
                     QPEMLVCDEAVSALDVLVQAQILALLNDLQARLGLTYLFISHDLAVVRQIADDVVVMC
                     RGRVVEHAHADDVFERPHDDYTRRLLDAIPGLA"
     gene            complement(11680..12645)
                     /locus_tag="VF874_01860"
     CDS             complement(11680..12645)
                     /locus_tag="VF874_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014348631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9174873.1"
                     /translation="MADGLRASGSSPGERPGQERYVSGPVDAAAVEADSVAPGGFWRE
                     AWRNLRTRPQFVVSALLIVFVLAMTMFPWLFTSADPTYADPDQSMLGPSSHHWFGTDL
                     QGHDVYARTVYGARASIAVGLGATTAVVLIGATLGALAGFYGGWLDAVVSRISDIFFG
                     IPLLLAAIVLGQVLHQRTVWTVIAVLAVFGWPQVARITRGAVIAGRNTDYVLAAKALG
                     VNRFSILMRHVVPNALGPVIVVATISLGIFIVTEATLSYLGVGLPPSTVSWGGDINLA
                     QARLRSGSPILFYPAGALAITVLAFMMMGDALRDALDPASRIRRL"
     gene            complement(12638..>13126)
                     /locus_tag="VF874_01865"
     CDS             complement(12638..>13126)
                     /locus_tag="VF874_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9174874.1"
                     /translation="LGFLAQFVFGVKLGIAPVTVGDRATFGRLLLPGFVLGAVSFAYV
                     VRLTRSAVSENAGADYVRTATAKGLSRPRVVTVHILRNSLIPVVTFLGVDVGALMAGA
                     IVTEGIFNIHGVGGALYQAITRQEAPTVVSIVTVLVVVYLLANLLVDVLYAALDPRIR
                     YG"
BASE COUNT         2180 a         4684 c         4429 g         1833 t
ORIGIN      
        1 gatcaacgcg ccgccgagcg ctcctatggg atcgccgagg acgatctgac gccgtgcatg
       61 agttggtcgt ttgtgtcgct gcgcaacgac tggaaccagc gcaagcacac tgtcgcggtg
      121 cgtgccgacg gcacgccgcg atttcgttgc cggcgggatc ggcgaccacg atcacccggg
      181 catggacgcg cccgatctgg cgtgtcggcc cagtgcgaca aggcgtcgaa actgtgctcg
      241 ggctgtgggg atctcaaggc agcggtcaac acggcgcgct acgcgcaacg tgaaacggac
      301 ccgagtaccg gggtcgtcac gggtggagcc gaccgtaagc cgccacctac cggtggcgct
      361 ggtggcaggg aaacccgaac ccgagccgaa gtaggcgaca acgccgatga cggtgggagt
      421 gcctccccca aaggagaggc tgcctgaaat gcccagccat acaaacactc acatttcagg
      481 taacggtgcg atggccagtt tcggccgcga cctgctcgcc agcgcgctcg ccggcaccga
      541 ccccaacgaa cacccgctgc ggcacgtcgc cgatctgccc gcacatcagg gcagctcggc
      601 tgcctggccg gcctgggcgg acggcgatgt cgtcagcgcg ttcgtcgccc agggcgtaac
      661 gacgccgtgg tcgcatcaag tgcgcgcggc gacactggca cagcagggcc ggcacgtggt
      721 ggtcagtacc ggaaccgcgt ccggcaagtc cctggcctat cagctgccga tcatgtgcgc
      781 gctggccacc gaccggcgcg ctcgggcgct gtatctgtca ccgaccaagg cgctgggcca
      841 cgatcagttg cgcgcagcgg cgtcactgac cggggcggtc gacagtctcg ccgacgtcgc
      901 gccggcgtcc tacgacggcg acagccccgc ggaggtgcgc cggttcgcgc gcgagcagtc
      961 gcggtgggtg ttctccaacc cggacatgat ccatctgtcg atgttgcgaa accacgcgcg
     1021 ctgggcggtg ttcctgcgca acctgcgctt cgtcgtcgtc gacgaatgtc attactaccg
     1081 aggcattttc ggttccaacg tggcgatggt tctgcgccgg ctgctgcggc tgtgcgggcg
     1141 ctacacggcc gcgccgaccg tcgtgttcgc cagcgcgacg acggcctcgc ccggtgcgac
     1201 ggcctcggag ctgatcggcc agaccgtcga ggaagtgacc gacgacgagt cgccccacgg
     1261 cgcacggacg gtcgcgctgt gggagccggc gttgcgcacc gatgtgacgg gcgagcacgg
     1321 tgcgccggtg cgccgctccg ccggtgccga agccgcacgc gtcatggcgg atctggttgt
     1381 cgaaggggcg cgaacgctga cgttcgtgcg gtcgcggcgc ggcgcggagc tcactgcgtt
     1441 gggcgcgcgg gctcggctgg ccgacgcggt gcccgccctc gtcgagcggg tcgcgtcgta
     1501 ccgcgccgga tatctcgccg aagatcgccg ggcgctggaa cgcgcgctgg ccagcggaga
     1561 gttggtgggg ctggccacca cgaacgcgct cgagctgggt gtggacatcg ccggattgga
     1621 tgccgtggtg ctggccggtt ttcccgggag tgtgacgtcg ttctggcagc aggcaggccg
     1681 gtcggggcgg cggggccaaa gcgcgctggt ggtgttgatc gcccgcgacg atccgctgga
     1741 cacctacctg gtgcaccacc cggaggcgtt gttggacaag ccaatcgaga tgacggtgat
     1801 cgacccgacc aatccgtacg tgctgggacc tcagttactt tgcgccgcaa cggaactgcc
     1861 gctgacggat gcggaggtac aggggtgggg cgctgtcgag gtcgcccgcg cgttggtcga
     1921 cgacggtctg ttgcgtcgcc ggggcagcgg ctatttcgcg gcgcccggtg tcgacccgca
     1981 tccggccgtg aacatccgcg gctcgagcgg cgaccagatc gcgatcctcg aagcggatac
     2041 gggtcggctg ctcggcggtg tcgaggcggg ccgggccgcg ttgtccgtgc atcccggcgc
     2101 ggtgtatctg caccagggcg agacgtacgt cgtcgacacg ctggacttcg acgaaggcgt
     2161 ggcgttcgtg cgcgtcgacg accccggcta cgccacgtac gcacgggagg tcaccgacat
     2221 cgcggtgacg gggccaggtg agcggtcgac cttcggtgac gtcgtgctcg ggctcgtccc
     2281 ggtcgccgtc actaaccagg tgatcgggta tctgcgccgc acgccggccg gggaggtgat
     2341 cgacttcgtc gagctggaca tgcctgcgcg cacgttggaa accgtggccg tcatgtacac
     2401 gatcacccca gaagcgctgg aacacaacgg cattgactgg tcgcaggtac ccggcgcgtt
     2461 gcacgccgcc gagcacgccg cgataggcct gctgccgctc gttgccagct gcgaccgcgg
     2521 cgacatcggc gggttgtcga cggctgtcgg gcccgacacc ggcttgccga ccgtgttcgt
     2581 ctacgacggc tatcccggcg gcgcgggatt cgccgaacgc ggattccgcc aggccgcgac
     2641 gtggctgtcg gcgacggcgg acgcgatcga cgcatgtgaa tgcccgcagg gttgcccctc
     2701 atgtgtgcag tcgccgaagt gcggcaacgg caatgaccca ctggacaaac atgccgctgt
     2761 gcgagtgctg cggctcgttc tgtcagaact gcccgcgcac gaggtccccc gacccatccg
     2821 tcggtgacgg ccgacccctc gacagccgtc tccacggatt gacaacagcc aaccgatggg
     2881 aacgctctcc tcgcctccga agacagcggt catgccgata acggcatacg cggcgaacgg
     2941 cttggcaacg cattccgttc cccatcccga ttgctcgggt gcacgcgaaa gttatcgtgt
     3001 ccgaacagga tgcgcaagtc ggcggactcg gtaaaccgca gtttgctggg ctaactaagc
     3061 cttctgacga cgaaatccgt agttgatggg acccgtcacg aggacgtgac gggtcccgcc
     3121 cgcgccacgg cgcgcgccgc gcctatgtcc cacgcggcca gcggcacgtg tgcgtcgacg
     3181 gtcacgacca cgtccagtgc gtcggcggtg catccggtga cggcggtgct catcgcgcgc
     3241 gccaccgccg ccgccttgtc gcatgccgcg gtgagccctg ccggcatgac accggccgcc
     3301 gccaacgcgg ccatgtcggc cgcggcctgc gcctggtgcc gagcgatcac cgcggacccc
     3361 agctgcacga cgccgactgt gacggccagc agcacggcca tcatcgacgc ggcaacgatg
     3421 ctggcagaac cgcggtcact gcgcgggcgg ctccgtggcc gacaccgcct cggctgtgat
     3481 cgagaggccg ggcagcagag ccgaatgcgc cgtgactcgc gccacgacga agtcgccgtc
     3541 gtggcgcaac gccagcaccg cgtcgtgtgg tgcaatgcgc cgcgccgcgg cctcggcgcg
     3601 ggcgtcgtcg ccgcgtgcca ccaacctggc cgcctcccgc gcggcgtcga tgcagcggac
     3661 ttgcgccgac acggcggtga cccctgcgat gcacagcagc agcacgacca ccagcgccgc
     3721 gatcgcgaac gccgcttcga ccgtgacgcc accgtcatcg tcggctagac gttggtgttc
     3781 aatgcgcggc tgatgatgtt ggtcagcgcg ttgacgatcg agtcgccggt caccacggtg
     3841 tagaggatcg ccccgaacgc cgcggcggcg atggtgccga tcgcgtactc cacggtcgac
     3901 atcccgtcct cggccgccca cagcaccgtc atccgtgcgc gcagatgttg ccatcgcgtg
     3961 gtcatcgtct tccccctcca tctcgttccc ttcaccacgg ccccgatccg agcacctggc
     4021 ctgccagccc ggcgaccacc ggcacgatgc ccaggcagac gaatgcgggc aggtagcaca
     4081 gccccaacgg gcccgcgatc agcaccgaag cccgctcagc ggtcgctcgg gctgcatcgg
     4141 cggcctcgtg gcgcacggac accgccagct cagcgacacc ctctgcgaga gccgagcccg
     4201 acgacgccga ccggcgcgcc aggcgcagca gcgactcgca gtgccggtcc ccgcttgcgg
     4261 gggtcgtcca cgcggccgcc gggtcggcgc ccagtgccag catgtcggcg gcgcggcgca
     4321 gcaacgcggc cagccgaggt ggcgacgccg gagcggtcgc ggcggccgcc gatgccaccg
     4381 ccatccccgc ggtcaggcac acggcgagca cgtcgaggct ggccgcgacg gcgagcgggt
     4441 cgggcttcgt gccggtcggc cgacgagccg gcgctcgcgt cgcgaggccg gcgcgacgcc
     4501 gcacgttcgt cggaccgggg gatatcagca cggccgccgc cagcagcacc gcggccagcg
     4561 tcatgccagc gcctgcccgg tgatgcggtc cgaccacagc agtccgcagc agaccagcga
     4621 aaccccgatc accagcagcc agccaccggc gccgcccgag acgagaaatc gcaccgggtg
     4681 tgcgccgagc agcccgccga gcgcaacacc ggccagcggc aggccggcga gcacggacgc
     4741 ggtggcccgt gcgcccgcca tgccggcgat cacctgtgcg tcgaagcgtg cccgttcgac
     4801 gatgtcgcgc tgggcggcgc gcatcagcgt cgccatcgtc aggccgtgtg tctgtccgag
     4861 ttcccagcac accgcgagcc gttcccagta cacgggccgg gccgcggcgc cagcctcgct
     4921 gagcagaccg gccggcacgt cggcgcccag caccgcccgc gcggccaccg cgcgaaacga
     4981 ctcgccgacg tggccgtcgc tctccgcggc ggcaatctgg aacgcggaaa caggatgggc
     5041 gcctgcgcgc aattcaccgg caaggacgtc cagagccgtc tccagcgcgc gagactcctg
     5101 ctcgatgcgc cgccgcgccg ctaggcttcg ccgacgcaag agcagcgtcg tcccgaggat
     5161 cgccgcagcg atcacgatcg acgccggcag cgctgcagcg gtcaccacac cggccgcgca
     5221 caccaccacg ccgaccgccc gcgagggcat catcgagcgg cgacgcgtga tcagtcccgc
     5281 ggcgacgagg cgatgacgcg acgaccacgg caccacgagc agcgccgcgg ccagcgccag
     5341 cgatgcggcg ggcagtccgg tcaccggaca ccccgtccgc tcagcaggtc ccgcaacagg
     5401 tcggcccccg gagccggtgc ggcgtcggcg tgccaagcgg tgacgacccg gaccggctcg
     5461 tcgccgtctc gccgcagcac cgcgatctcg tcgaggcgcc ggccgccgtt ctggccgcgg
     5521 cgcacatgca gcacgacctg caccgcggcg gccaactggc tgtgcacagc ggcgcggtcc
     5581 agcccgccca gcgcggcgag ggcctcgagc cgggccggca cctcggacgg gcggttggca
     5641 tggacagtgc ccgcgccgcc gtcgtggccg gtgttcaacg cggccagcag gtcgacgacc
     5701 tcggcgccgc ggacctcccc gacgacgatc cgatccggac gcatgcgcag cgcttgcttg
     5761 acgaggtcgc ggacggtgac ctcaccgact ccttccacgt tggcggcgcg ggccaccaag
     5821 ttgacgagat gcggatgacg aggtgccagc tcggcggcgt cctcgacgca gacgatgcgc
     5881 tcgtcggccg acaccgcgcc gagagtggcg gccagcagcg tcgtctttcc tgcgcccgtc
     5941 ccgcccgtga ccaggaacgc cagccgcgcg gcgatgatcc ggtcgaccag ctccgcggcg
     6001 ccccgcgcga tggttccggc ctgcaccaac gccggcaggt cttgcgtggc gggacgcaac
     6061 acgcgcagcg acagacaggt tccgccggct gccagcggcg gcaagatcgc gtgcagccga
     6121 accgcgaact ggccggtgcc cagtccgctc aggtgcccgt cgatccatgg ctgcgcgtcg
     6181 tcgagccgac gtcccgcgga aagggcaagc cgctgcgcga gccggcggac cgcagcctca
     6241 tcggtgaagt gaatatcggt gcgccgcaac ccatttccat catcgaccca cactgcgtcc
     6301 gggccggtca ccaacacgtc ggtggtgccg tcggcgcaca gcagagcctc gaggatgccg
     6361 gcgccggtca gttctgtctg cagcacccgc aggtgcgtca gcacctcggt gtcgccgagc
     6421 accccgcctg actctgcccg gatcgccgcg gcgaccacat tgggacgcag gggcgcggac
     6481 tctgctgcca gccgctcccg cacacggtcg atcaacgacg ggttcacgca gcctccaagg
     6541 cgacctggcg accgagcact ccgagcactc gccgagcggt tgtcaacagc ggcgacctcc
     6601 ggcgcaggcg caggccgaca cgctcgaggc gctcggccag gttgggttcg gggcgcatgg
     6661 ccgcaagcag cggcaatccg gcggcgcgag ccacgtcggc agcgcgcaac ccaccgggag
     6721 acggcccccg cacgacgagg ccgacgttcg ggttgatgcc gatcagcgcc gcggccatcg
     6781 ctgaggctgc cgcgcacgat cgcacgtcgc acgcggcgac gagaacaacc agctcggccg
     6841 cgtccagcgc ggcctccgcc gcctcggtga agcgtcgcgg cagatcgcac acaacggtca
     6901 caccgccacg ccgacccgca tcgacgatcg cgagcaacga cccgccggtg atgtcactcc
     6961 cgtgtcgcgc acccgacagc accgcgacgc ggtggtggca gggcagcgcg tcgcgaaccg
     7021 cggcgaggct gagccgcccg ttctcgaccg cgacgtccgg ccaccgcaga cccggttccc
     7081 cctcgaggcc gaggagcaga tccacgccac cactccacgg gtcgacgtcc accaacaacg
     7141 cgtcggcagc cgcgatcgcc aacgcggcgc tgaagatcga tgctcccccg ccgcctcggc
     7201 cgctgatcac tgcgactgcg cttccgcggg ccccttggtc gaagtcgccc gcgtcggaaa
     7261 gcaccgcgac tagttcgccc tcttcgtccg gcagccgcaa tacatgttct gccccaaccg
     7321 atatggcggc ctgccacgtc tcctgctgtg gttcaccgac gaccagcaag atcacccgcg
     7381 gccgccgcgg cattccgctg gcggcgccgc gacgagccgc cgcctcatcg agaacgaccg
     7441 cggtggccgc gctccactcg cgtcggctcg cagctcgcga agccggacag tagacgacgc
     7501 gcacgccgac cgcggccgcg actcgatcaa cgccgtcgcg cagcgcggga tcgtcgacga
     7561 cgcccaggac gccactggtg ggtctcatca gcccagcctg cgtcccgtgt gagcaacgat
     7621 tccagtaggg cgacatgatc tgtgcacaga cccgaagctg tggataacga acgacgtgaa
     7681 aacgggattc cgaaactact gaccgcccgc tgaaggggtt ggctcgcgga tatcttttgc
     7741 cccaaagggg acgacccccg ccaggggggg aggaggcgag ggtcgtcgtg tatcagcccc
     7801 ggggggtcgg gctgatacac acccggcata agccgagtaa tgctcactat acacacgact
     7861 cgggacaatg gcgcaatagt cacgcgcatc cgataacact gagccagcac ggaattgcag
     7921 gttcagacag gtgacttgat ctgccatttc acaggcacgg aatgcgccgt cggggcacca
     7981 ccctatgcta agcccgtgac cgcctccgat caggccgcag caccgctcac cacgacgcag
     8041 gtcacgccgt cagcggactg tgacccggag agtcccacca agaccgcagc gtttttcgac
     8101 ttggacaaga cggtcatcgc gaagtccagc acgcttgcgt tcagcaaacc tttcttcgac
     8161 caaggactga taaatcggcg cacggttttg aagtccagtt acgcacaatt tctgttcctg
     8221 atgtcgggcg ccgaccacga ccagatggac cggatgcggt cgtatctgac gaacatgtgc
     8281 accggctgga acgtcgagca agtgaagtcg attgtggcag aaacgctgca cgacatcgtc
     8341 aacccgctcg tgttcgccga agccgccgac ctgatcgccg accacaagct ctgcggccgc
     8401 gacgtcgtgg tggtgtcggc ctctggcgag gagatcgtcg cgccgatcgc ccgcgcgctg
     8461 ggcgcgacgc acgcgatggc gacacggatg gtcgtcgagg acggcaaata caccggcgag
     8521 gtcgcgttct actgctacgg cgagggcaag gcgcaggcga tccgggaact cgccgaccgc
     8581 gaggggtacc cgctggagca ctgctacgcc tactccgact cgatcaccga cctgccgatg
     8641 ctggagaccg tcggccatcc cgcggtggtc aacccggacc gcgggctgcg caaggaagcc
     8701 gcctcgcggg gctggccggt gctgacgttc tccaagcccg tgtcgctgcg ggatcggatt
     8761 ccagcgccgt cgacggccgc ggtcgccacc accgccgcgg tcggagtcag cgcggtggct
     8821 gccggggcgt tgacgtattc gctgctgcgg cgcttcgtac tctgatgggt gccggcctcc
     8881 gcgcgagcag acgtaaacgc cccgattccg ttgccagaat cggggcgttt gtgtctactc
     8941 ggtttctggc tggtttcaat gccggtctcg gcgcaccgaa accttgatgt gacggtggtc
     9001 acgtagtaca aaggaaccac ggaagcccgg tgaggccaag gtcgagtcgg aagagaaggg
     9061 tcgatctccc ggaccggact cccagcgcgg atcccggcac ccacgcggag cacgccgcat
     9121 aaaggcagaa gtgttgcggg cctgcgtaat tgcgaaaagc gaagaactcg acaacccttt
     9181 gggtagggca gtggttctgg cgcatcgcga gacgccgagg ccacccacgc agccccagct
     9241 gcacgcctgg taaccgaagg tccgcgctag cgggcggcga gccgaagcga ttcggatcgc
     9301 cgcccgcttc atgtgcgctt cgaggccgcc tcggccatcg accacgcttc gccggcgccc
     9361 tcctccagcg ctcggcagca cagcagcatc cactgcctca gaccgtcagc gctgccggta
     9421 gcgaactgtt gcgcagccgc gcggtattcg ctggcgtgtc gcatccaata gacctcggga
     9481 acagccagtc cgtgcgggtc cagcccactg gcctgcgtca ccaaccggga caccgcgcgg
     9541 gccaccactc cgttggcgct gccgaacggc ctcagcgtca agatctcgcc gtgggccgcc
     9601 gccgccagca ccggcgctgg tgcggagctt ccgccggtca tcaggccggc cagcagttcc
     9661 agccggggtg ccacaccctc gcgcggccgg cctagcgcgg cgtcgtcaac cagatcggcg
     9721 gccgcgagcg catgcagtcg ggccagtgcc tgcagcggtg cccgctgcca gacgctgaca
     9781 agcgacgtct ggccgccttc cagcgcctcg gcaacgcgca gcgcgccggc caggatgggt
     9841 ccgctgtcca gcccgtcggc cgggaatcgc ggcgcgccgc cgtcgagaat cgatgacgcc
     9901 tgggctgccc gcacggccgc ttcggccgca gtcgccggcc atccgcgcag gttgacccgg
     9961 tgccgatgca ccttgcccag cgcgtcgcga acacgttcgc ccgcgtcggc cacgccgggc
    10021 aggtcggcca gcggggcgag cggatctgtc atccccgtca cgctaagccg gggatcgcgt
    10081 cgagcaggcg gcgggtgtag tcgtcgtgag gccgctcgaa cacgtcatcg gcgtgcgcat
    10141 gctcaaccac ccggccgcga cacatcacga cgacgtcgtc ggcgatctgg cggaccaccg
    10201 cgaggtcatg gctgatgaac aggtaggtca ggccgaggcg cgcttgcaga tcgttgagca
    10261 acgcgagaat ctgcgcctgc accaggacgt cgagtgccga caccgcctcg tcgcacacca
    10321 gcatctccgg ttgcagcgcc agggcgcggg cgatcgcgac gcgctgacgc tgaccgccgg
    10381 agagctcccg cgggaaccgg cgcagcacgc tcgacggcaa cgccacgtgg tccagcaggt
    10441 cccgcaccgc gcgctgacgg tcgacgcgac tgccgatgcc gtgggtgcgt agcggctcct
    10501 cgatcgtgcg gtacaccgag tacatgggat ccaaactggc gtaaggattt tggaacaccg
    10561 gctggactct gcgacgaaat gccttggccg cggcgccgtt cagcgtagcg acgtcgcggc
    10621 cgtcgaacgt gaccgttccc gaggtgggcg ccagcagggc gagcaccatc ctcgcgaccg
    10681 tggtcttacc cgatcccgat tcgccgacga cggcggtggt cgtcgcacga cgcacccgga
    10741 acgacacgtc gtcgacggcc gtgacgtcgc gctggcgcca cggcgcaccg acgcggtaca
    10801 ccttggtgac gttggacaac accagcatgt cgtcgatcgg ctgcccggca tgcgcggccg
    10861 cctccgttac cactcgtcga cggactgccg cgccgcggcg catcgaaagc gacggcgccg
    10921 acgccaccag ccgctgcgtg tactcgtgct gcggatcgtc gatgatcgcc tgcgccgggc
    10981 cggattccac cactgtcccg cgatacatca cgaccaggtg ctccgcgcgt tcggcggcca
    11041 gcgccaggtc gtgggtgatc agcaacacgg cggtgcctcg ctggccggcc agctcgtcta
    11101 ggtggtccaa gacttgtttc tgcacggtga cgtcgagcgc cgacgtcggc tcatcggcga
    11161 tcagcaaccg cggctgaccc gccagcccga tcgcgatcag cgctcgctga cacatcccac
    11221 cagacagctg atgcgggtag gagcccgcgc gcatcgccga atccgtcatg ccgacgtcgt
    11281 ccagcaactc aacggctctc ttcggcgcgg cgcgccggtc ggctatgccg ttggcccgca
    11341 acgtttcccg aatctggaac ccgattgtcc agaccgggtt caagttggtc atcgggtcct
    11401 gcggcacata tccgatctgc cgtccgcgga tcgtctcgag ccgccgccgg ccggcgtggg
    11461 tgatgtcgtg tccgtcaaag acgatgcggc cctgggtcac tcggccgcct ggtgcgagga
    11521 ggccgagcac cgcagctgcc gtggtcgact ttccggatcc tgactcaccc accagcgcga
    11581 cggtctggcc gggatagacg gccaaatgag cgttacgcac cgcgggcagg tcggtgccga
    11641 agcacacttg cagcccatcg atcgacagca gcggctccgt catagtcgcc ggattcggga
    11701 ggccggatcg agcgcgtcgc gcagtgcgtc gcccatcatc atgaacgcga gcaccgtgat
    11761 cgccagcgcg ccagcgggat agaacaggat gggcgaaccg gaccgaaggc gcgcctgggc
    11821 gagattgatg tccccgcccc acgacaccgt cgacggcggc agcccgacac cgagatacga
    11881 aagtgtggcc tcggtgacga tgaagatgcc cagcgagatc gtcgcgacca cgatcaccgg
    11941 gccgagtgca ttcgggacga cgtggcgcat taagatactg aaccggttga cgcccaacgc
    12001 ctttgcggcc aagacgtagt ccgtgttccg tccggcgatg accgcgccgc gcgtgatacg
    12061 tgcgacctgc ggccagccga acacggccag caccgcgatc accgtccaca cggtgcgctg
    12121 atgcagcacc tgccccagca cgatcgcggc cagcagcagc gggataccga agaagatgtc
    12181 gctgatccgg gagacgaccg catcgagcca gccgccgtag aaacccgcca gcgcgccgag
    12241 cgtcgccccg atcagcacca ccgcggtcgt cgcacccagc cccacggcga tcgacgcgcg
    12301 cgctccgtac acggtgcggg catagacgtc gtggccctgc aagtcggtgc cgaaccaatg
    12361 gtgcgacgac ggtcccagca tgctctggtc ggggtccgcg tacgtcgggt ccgcgctggt
    12421 gaacaaccac ggaaacatcg tcatcgcgag gacgaacacg atcagcagcg ccgagacgac
    12481 gaactgcggc cgggttcgca ggttacgcca cgcctcccgc caaaatccgc ccggcgcaac
    12541 agaatccgct tccaccgcag ccgcgtccac cggcccgctc acataccgct cctggcccgg
    12601 gcgctcacct ggggatgagc cgcttgcgcg aagaccatca gccatagcgg atcctcggat
    12661 caagcgcggc gtagagcaca tcgacgagaa ggttggccag caggtagacg actaccagca
    12721 ccgtcacgat cgacaccacg gtcggcgcct cctggcgggt gatcgcctga tacagcgcgc
    12781 cgccgacgcc gtggatgttg aagatgccct cggtgacgat ggcgccggcc atcagcgcgc
    12841 cgacgtcgac acccaggaat gtcaccacgg gaatcagcga gttgcgcaga atgtgcacgg
    12901 tgacgacccg cgggcgggac agtcccttgg ccgtcgcggt ccgcacgtag tcggcaccgg
    12961 cgttctcgct caccgccgag cgggtcagcc tgaccacgta ggcgaacgac accgcaccaa
    13021 gcacgaagcc cggcaacaac agtcggccga acgtcgcccg atccccgacg gtgaccggcg
    13081 cgatgccgag cttgacgccg aacacgaact gcgccaggaa cccgag
//
LOCUS       DASWBF010000065         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1055969,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000065 DASWBF010000000
VERSION     DASWBF010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055969"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1472
                     /locus_tag="VF874_01870"
     CDS             285..1472
                     /locus_tag="VF874_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9174875.1"
                     /translation="MFDDFEDVRDLSMLEVPAVGSLAVTGSKWEPYRLLDPEGRPVGA
                     VAAFFGDLLAAGRSEATVRSYGMDLLRWFRFLWAVEVAWNRATRLEARDFCRWLAITT
                     GPKGSGRATPYSVSVRVHSETVLRSFYDFHRDVGTGPMVNPFPLGRSQRGGRAHAHHN
                     PMEPRHNERAGLYRPRVVKRIPRSIPDAEFNEIFARLPSHRDRALVAFYVSTGARASE
                     LLTVTQGGVDPGRQLITVVRKGSCAEQELPASSDAFVWLRLYQVEMEGLVPSGRTKPL
                     WWTRRKPFRPLTYHAAHRMFERVNADVGTGVTLHALRHTAAYRMAQDPAMPLTDVQYV
                     LGHAQLTTTQIYLTPRKEDVIERILAHHAEQTRKAAQKDTPPAAGYRPETLDVLFAAA
                     RRR"
     gene            1628..>3117
                     /locus_tag="VF874_01875"
     CDS             1628..>3117
                     /locus_tag="VF874_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9174876.1"
                     /translation="MDGVVAAATAELPTQRDREVRRWGVPLLLDWLADQLGDTWQQRW
                     MASGADAAGPDWAQHPEQWLRRQGKYPENRLRLMTSSLLAVVGADVVRPSLAWLLTSG
                     KKRNLVRHMVYGRDGAGFDRLRRLCEQDPAISHVAMGDTMFRSAVIVATKGGLLADVT
                     VGDVLEILDTECAVRDRVYSASATFRMLREAGVLGADTPTLHQIRSLGQRSPAQLVDR
                     YPIACRPIRDLLVAYLAERQPAIDYTTLVSLAYHLVRCFWFDLERHHPGIDSLRLPRE
                     VAGAWKRRLRTKTTTMIRDDERVEVESERLGYLDTLATVRAFYLDLAEWGLDDPVRWG
                     VWVAPCPINQEDLVRRKSVRRRKARMDSRTRERLPVLPALVEAVDRWRRESRQLLDTG
                     REAQPGKQFTAAGKTLTRIDRQHAAADKVWVRDLDTGKPRLLNLDEEHAFWAWAVIEV
                     FRHTGVRAEELLELSHHSLVQYRLPSTGELVPLLQVAPSKTDAERLL"
BASE COUNT          515 a          984 c         1089 g          529 t
ORIGIN      
        1 ttccgcgcag ctggcatacg gcctcgatat gtcgcagttg ccaaacatca ggaagccgtt
       61 ccgggcgtca cgtaggtggc ggtcacagcg cggcgagctc tcgtcccgca aacattcatg
      121 cggagcgggt gttgcccggc actcaaaatg accatgacgg cgtcactttc ggtgaggacg
      181 aacagctggc gttggtgtgc atgctgacct gttgactgaa ctaattccga cgttccggca
      241 tgtcagcgcc gtgatcggcg ggtagcggtc ctaccgtcgg tgccgtgttc gacgacttcg
      301 aggacgtgcg ggatctgtcg atgttggagg ttccggctgt ggggtctctg gcggtgacgg
      361 gctcgaagtg ggaaccgtat cggctgctgg atccagaggg gcgaccggtc ggagcggtcg
      421 ctgcattctt tggcgacctg ctggctgcag ggcggagcga agcgacggtt cgctcgtatg
      481 gcatggacct gctgcggtgg ttccggttcc tgtgggcggt tgaggtggcg tggaaccgtg
      541 cgacccggct ggaggcccgc gatttctgcc gatggcttgc gatcacgacc ggcccgaaag
      601 gttctggtcg agcgacgccg tattcggtgt cggtgcgggt gcacagcgaa acggtgctgc
      661 gctccttcta tgatttccac cgcgacgtcg gcacgggccc gatggtgaat ccgtttccgc
      721 tgggccggtc acagcgcggc ggccgggcgc acgcgcatca caacccgatg gagccgcgtc
      781 acaacgagcg ggcagggttg tatcggcctc gggtggtcaa acggatcccg cgcagcattc
      841 cggatgcgga gttcaacgaa atcttcgcgc ggctaccctc gcatcgggat cgggcgttgg
      901 tggcgttcta tgtgtccacc ggggcgcggg cttcggagct gctgaccgtg acacagggcg
      961 gtgtggatcc tgggcggcag ttgatcactg tggtgcgcaa gggctcctgt gcagagcagg
     1021 agctgccagc gtcatcggac gcgttcgtgt ggctgcggtt gtatcaggtg gagatggagg
     1081 ggctggtccc gagcggacga accaaaccgt tgtggtggac gcgacggaag ccgtttcgcc
     1141 cgttgaccta tcacgcagca caccgcatgt tcgagcgagt gaacgccgac gtcggcaccg
     1201 gcgtcacgtt gcatgcgctg cggcataccg cggcctaccg gatggcgcag gatccggcga
     1261 tgccgctcac cgatgtgcag tatgtccttg ggcacgcgca gctgaccacc acgcagatct
     1321 acctgactcc gcgcaaggag gacgtgatcg aacgcatact cgcccaccac gccgaacaga
     1381 cccggaaagc cgcccagaag gacacaccgc cggccgcggg ctatcggccc gagacgctgg
     1441 atgtgctgtt cgccgcggcg cggaggcgct aatgaccatc gtcgagacga tccccgcggc
     1501 gaggaccgca acccctgtcg gcgcccgatc gctcgaatca gcgtccacgc aaccggattg
     1561 gcgaactcgc tatcccgcgc gtgtggtcgg gacggactgg ccggctacat gcggtgatcg
     1621 tgccgaggtg gatggggtcg tggccgccgc gacggccgag ttgccgacgc agcgggatcg
     1681 ggaagtccgc cgctggggcg tgccgctgct gttggactgg ctcgccgacc agcttggcga
     1741 cacctggcaa cagcggtgga tggccagcgg cgccgacgcc gcaggcccgg actgggcgca
     1801 gcatcccgag caatggctgc gccgccaggg aaagtacccc gagaaccggt tgcggttgat
     1861 gaccagctca ctgctggcgg tggtcggcgc cgacgtggtg cgaccatcgc tggcatggct
     1921 gttgaccagc gggaagaaac gcaatttggt ccgccacatg gtctacggcc gcgacggggc
     1981 gggcttcgac cggctgcgtc gattgtgcga gcaggacccg gcgatcagcc atgtggctat
     2041 gggagacacc atgtttcgct ccgcagtgat cgtcgccacc aagggcgggc tgctggccga
     2101 tgtcacagtc ggcgacgtgc tggagatcct cgacaccgaa tgcgcagtgc gcgatcgagt
     2161 gtattcggcg tcggcgacat ttcggatgct gcgcgaagcg ggcgtactcg gcgctgacac
     2221 gcccacactg catcagatcc gcagtctcgg ccaacgctcg cccgcccagc tcgtcgaccg
     2281 ttacccgatc gcttgccggc cgatccggga cctgctcgtg gcctacctgg ccgagcgaca
     2341 accggcgatc gactacacca ccctggtcag cctggcgtat cacctagtgc ggtgcttctg
     2401 gttcgacctt gagcggcatc atcccggcat cgactcgctg cggctgccgc gtgaggtcgc
     2461 cggcgcgtgg aagcggcggc tgcgcaccaa aaccaccacg atgatccgtg acgacgagcg
     2521 ggtcgaggtc gaatccgaac gcctcggcta tctcgacacc ctggccacag tgcgggcctt
     2581 ctacctagac ctggccgaat ggggcctcga tgacccggtc cggtggggtg tgtgggtggc
     2641 gccgtgcccg atcaaccagg aggacctggt gcggcgcaag tccgttcgcc gccgcaaggc
     2701 acgcatggat agccgcaccc gtgaacggct gccggtgttg ccggccctgg tcgaggccgt
     2761 cgaccggtgg cggcgcgagt cacggcagct gctcgacacc ggacgagagg cccagccggg
     2821 caagcagttc accgccgctg gaaagaccct gaccaggatc gaccgtcagc acgcggccgc
     2881 ggacaaagtc tgggtacgcg acctcgacac cggcaaacca cgactgctca acctcgacga
     2941 ggagcacgcc ttctgggcgt gggcggtcat cgaggtattc cgccacaccg gtgtgcgggc
     3001 tgaggagctg ctcgaactga gccaccacag cctggtccag taccggctgc ccagcaccgg
     3061 cgagctggtg ccgctgctgc aggtcgctcc atccaagacc gacgccgaac ggttgct
//
LOCUS       DASWBF010000066         3977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_135239,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000066 DASWBF010000000
VERSION     DASWBF010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135239"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..886)
                     /locus_tag="VF874_01880"
     CDS             complement(227..886)
                     /locus_tag="VF874_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174877.1"
                     /translation="MGGERSMTAAARRPLHIIADTDEHDDTAAIGAHAGVDVEIPETW
                     QSQTVLNQWESEHQLIGALMWMSAEQARPILELVPDTAIWRPTIRWAYQIIRRLVHDG
                     RDPDPVTVLATAKHQPCSEAVNPEQPPTPVRHHKLAVYLANAYTQTISAVAAESYARE
                     VLDEAYRRAFRFHGIRMQQFADCGAERGHLTAEFIAIRDDLADWRRRAEAAAKPGWSQ
                     P"
     gene            complement(865..1152)
                     /locus_tag="VF874_01885"
     CDS             complement(865..1152)
                     /locus_tag="VF874_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174878.1"
                     /translation="MLTAAQAEGRCWFSAGTLLREAAHLAAVDPITQPDQALLETLRR
                     DVITRGHADTTPGPAELAAAVQLAAPADTPAAHTNALIWWYTLLTWGVSAA"
     gene            complement(1146..1523)
                     /locus_tag="VF874_01890"
     CDS             complement(1146..1523)
                     /locus_tag="VF874_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174879.1"
                     /translation="MYQLTIDAGAARIVSEHPDWPTAHHHLIDYVRGQDYYLQLIQCG
                     QPHTSYALLDLRDIAEEQRCRAEGRQPRTAGHAVIEELTPTEVVDPPYYAAAAARAGS
                     TTTANCGATQPTPTAQPATRWQC"
     gene            complement(1555..1788)
                     /gene="nrdH"
                     /locus_tag="VF874_01895"
     CDS             complement(1555..1788)
                     /gene="nrdH"
                     /locus_tag="VF874_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEX9174880.1"
                     /translation="MEAITVYTKPACMQCNATFRALDHAEIAYTKIDITQDRTARDYV
                     LSLGYLSLPVVYVSPALHWSGFRPEKLRSLSAA"
     gene            complement(2376..3425)
                     /locus_tag="VF874_01900"
     CDS             complement(2376..3425)
                     /locus_tag="VF874_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HEX9174881.1"
                     /translation="MAHELDSTNGQVSFANSRTDAWHRLGQSVGHAMTAREALDAAHL
                     SGWNVHKMALQIPREPIITENGVTTPTPIEVPDQFATVRTNPIHGGIDYLGVVGSKYE
                     PVQNEASCTLLDALTEEGGAVYETAGTLRGGRDTFVTMKLPKSMDFEGRDGSKDRTDW
                     YLAALNSHDGSSAFRFLLTPIRIVCANTQAAAIARAKASFAIRHTGGARGAINEARTA
                     LGLSWRYMEAFETQAAALYAAPMELEELRDFAAELVKVDQAPSAAARTRRREQASGIV
                     KLFVSSPTIAPTPIAGTRWAAYNAVTEYVDHVAPVRGTRSRAEAADVRALRAVTTGST
                     AQMLKVEAFGMLQTI"
     gene            3743..3952
                     /locus_tag="VF874_01905"
     CDS             3743..3952
                     /locus_tag="VF874_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174882.1"
                     /translation="MAARRYAVLCPVIAGYGGVVLEDALRRCLALDRPTPSLSLLHWV
                     VTGTWPDPVVRVDVGFPPPGAAPLL"
BASE COUNT          532 a         1122 c         1514 g          809 t
ORIGIN      
        1 ccgatgacgc gccaaattgt ccgccgctag cgtcgctccg gggtgcagcg gatggacatt
       61 catactgagt tcgctggggc ccggccatcg cctcgggact gcgaccggag cagccgaagc
      121 gctgacttct ttcctgttga gtgtgtgggt ggccgggcag cgccggcccg tcgcgcgatc
      181 gccgcgtcga gcgcggtgcg ctgggtggcg gtgtgtgcgc ggcggatcat ggttggctcc
      241 agcccggttt ggcggccgct tcggctcggc gccgccagtc ggcgaggtcg tcgcggatcg
      301 cgatgaattc cgcggtgagg tggccgcgct cggcgccgca gtcggcgaac tgctgcatgc
      361 ggatcccgtg gaaccggaag gcgcgacggt atgcctcgtc gaggacctcg cgggcgtagc
      421 tttcggccgc gaccgcactg atggtctggg tgtaggcgtt ggcgaggtag accgcgagtt
      481 tgtggtggcg caccggggtg ggtggctgtt cggggttgac ggcttcgctg cacggctggt
      541 gcttcgcggt ggcgagaact gtgacggggt cggggtcgcg gccgtcgtgg acgagtcggc
      601 ggatgatctg gtaggcccat cggatggtgg gccgccagat ggcggtgtcg gggacgagct
      661 cgaggatggg cctggcctgt tcggctgaca tccacatcag agcgccgatg agttggtgtt
      721 cggactccca ctggttgaga accgtttggg attgccatgt ttcggggatc tcgacgtcga
      781 cgccggcatg agcgccgatg gctgcggtgt cgtcgtgttc gtcggtgtcg gcgatgatat
      841 gcaggggtcg gcgggcggcg gcggtcatgc tgcgctcacc ccccatgtca gcagcgtgta
      901 ccaccagatc agcgcgttgg tatgagcggc gggcgtatcg gcgggtgctg ccagttgcac
      961 ggcggcggcc agttcggctg ggccgggggt ggtgtcagcg tggccgcggg tgatgacgtc
     1021 gcgccgcagg gtctccagca gggcctggtc gggttgggtg atggggtcga cggcggccag
     1081 gtgggcggct tcgcgcagca gggtgccggc ggagaaccag cagcggccct cggcttgggc
     1141 tgcggtcagc actgccagcg ggtagcgggt tgcgctgtcg gtgtcggctg cgtggcccca
     1201 cagttcgcgg tggtcgtcga gccagctcgc gcggctgcgg cggcgtagta gggcgggtcg
     1261 acgacctcgg tcggggtgag ttcttcgatg acggcatgtc cggcggtgcg cggttggcgt
     1321 ccttcggcgc ggcagcgctg ctcctcggcg atgtcacgta ggtcgagcag cgcgtagctg
     1381 gtgtgtggtt ggccgcactg gatgagctgc aggtagtagt cctggccacg tacgtagtcg
     1441 atcaggtggt ggtgagcggt gggccagtcg gggtgctcgc tgacgatgcg ggcggcgccg
     1501 gcgtcgatgg tgagttggta catggcgggt cctcggggtg gtggcgggcg gggattatgc
     1561 cgcgctgagg ctgcgtagtt tctcgggccg gaatcccgac cagtgcaggg cgggggagac
     1621 gtagacgaca ggaaggctca gatagccgag cgacagtacg tagtcacgtg cggtccggtc
     1681 ttgggtgatg tcaatttttg tgtaggcgat ttcggcgtga tctagcgcac gaaatgtcgc
     1741 gttgcattgc atgcaagcgg gcttagtata aacggtaatg gcttccattc ggagggtcct
     1801 ttctggcggg tttatcgctg ttccgatttt accgcattaa ttggcatggt ctcaacactt
     1861 ttttggcgcc ctgagacgtt gtcgttatgt attttcagtg aagccgaaaa tggtttatcg
     1921 acatgattgt caccggtcct gtcctccttg ctctgtctgt gcgccagcgg ttttcgtcgg
     1981 tgccgtttcg tatgatttct ttcgtctgtt gtgcgcccgt gtccgggccg attgtttttc
     2041 tggcggtatc gccgcaattg cttagaccgg cggtcgtccg cgtagcgcaa tggtccggcg
     2101 gaaaagtttt ccgcaagcgc atgcgagcat gaaacggaaa cttttctagc cgaggtttac
     2161 cgtggccttg cgcgcagcgg acagccggtc ataattgcaa aggcgagagc gtcgacggtt
     2221 gaaaatgcag cagcgccgcg ggcgcttccg ccggccagcg gggtcgccgg gccgccggcg
     2281 gcggcccgtg cgctggcgcc ggcgcaaacc cccgcctgac cggttggcct ggtgggcagg
     2341 tgcgtggctg ccctgtccac cgggcgcggt cggggttaga tggtttgcag catcccgaag
     2401 gcctcgactt tcagcatttg ggcggtgctg ccggtggtga cagcccgcag ggcgcggacg
     2461 tcggcggctt cggcgcggct gcgcgttcca cgcactggag ccacgtggtc gacatactcg
     2521 gtcactgcgt tataagccgc ccaacgggtc ccggcgatcg gggtcggcgc gatggtgggg
     2581 gaggacacga acagtttcac gatgccgctg gcctgttcac gtcggcgggt tcgagctgcc
     2641 gcgctggggg cctggtcgac cttgaccagt tcagccgcga agtcgcggag ctcctcgagc
     2701 tccatgggcg cggcgtagag cgcagcggct tgggtctcga aggcttccat gtagcgccag
     2761 ctcagcccga gagccgtgcg tgcctcgttg attgcgccgc gggccccgcc ggtgtgacgg
     2821 attgcgaaac ttgctttggc gcgggcgatg gcagcggcct gggtgttcgc gcagacaatt
     2881 cggatcggcg tcaacaggaa ccgaaacgcc gacgatccgt catgggaatt gagcgctgcc
     2941 agataccaat ccgtgcgatc tttggacccg tcgcggcctt caaagtccat cgatttcggc
     3001 agcttcatcg tgacgaaggt gtctcggccg ccgcgcagcg tcccggctgt ctcatagacc
     3061 gccccgcctt cctcggtgag ggcgtcgagc agggtgcaag atgcctcgtt ttggacgggc
     3121 tcgtacttgc tgccgaccac gccgaggtag tcgatgccgc cgtggatggg atttgttcgc
     3181 acggtggcga actggtcggg gacctcgatg ggtgtcgggg tcgtcacgcc gttttcggtg
     3241 atgatcggct cgcgggggat ctgcagcgcc atcttgtgca cattccagcc ggacagatgc
     3301 gcggcgtcga gcgcttcgcg tgcggtcatc gcgtgtccga ctgactggcc gagtcgatgc
     3361 caggcgtcgg tgcgtgaatt ggcaaaggac acctgcccgt tggtggagtc gagttcgtga
     3421 gccatggtga gattcctttc ggtttggggc gttgccgccc gttcttgttt gccgtctggc
     3481 actggagatt ccgaccggcg ttgaggccgc gggagtgcaa ggttcgggcg cgcagttttc
     3541 ccagcgaccc gtaggggagc ggcccgggga aaagttcgcg agcccgtggg cccaaagcgt
     3601 cagcgcaggc cgtggccttg cgcgaagcgg ccaccggtca aaatcgaaag gccagaaggc
     3661 aaaccgagat tcggcccgcg gtggcggggc cgtcagcagc gccgcaggcg cttctggcta
     3721 gttcgggttg gccgtgccgc cggtggcggc gcgacgatat gcggtcctgt gcccggtgat
     3781 cgcgggttac ggtggggtgg tgttggaaga cgcgttgcgc cggtgcctgg cgctggatcg
     3841 cccgaccccg tcgctgtcgc tgctgcactg ggtggtgacg ggcacctggc ctgacccggt
     3901 tgtacgggtc gacgtgggtt tcccaccgcc cggcgcggcg ccgctgctct gatcggcccc
     3961 agcacagcag tgggggc
//
LOCUS       DASWBF010000067         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1190698,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000067 DASWBF010000000
VERSION     DASWBF010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190698"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VF874_01910"
     CDS             <1..188
                     /locus_tag="VF874_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174883.1"
                     /translation="GANQWAGVKTYLDAVTDYAAAPVIAKYTGVLAAADVFTTSTTLV
                     VLESIRLDLGVGSQVHP"
     gene            241..1752
                     /locus_tag="VF874_01915"
     CDS             241..1752
                     /locus_tag="VF874_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007534437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9174884.1"
                     /translation="MASDAELTAQGQQEDHEDQLDERPSRRWWALAVIAIAQLMVVLD
                     GTVVTIALPSAQRDLGISDADRQWTITAYTLAFGGLLLLGGRIADYQGRKRSFLVGLL
                     GFAAASALGGAAQNGATLFGARALQGAFAALLAPAALSLISVTFTEVRDRSRAFAVYG
                     AISGGGAAIGLILGGTLTEYASWRWCLLINVPIAIVAFIAGTILITESKSGGGSSYDI
                     PGAILGTAGLVALVYGFTQAAKTGVGWLSVQAISCFVSAGILLVAFVLVELRSASPLL
                     PMRVVLERNRGGSYLASLLVFAGLFAMFLFLSYYFQYNLGYSALKSGIAFLPFSAGII
                     VTSAVVSGLLPRTGPKPLMLIGLAMVATGLATFTRISDTSSWVSTVLPGELLMSVGLA
                     LVIVPLSNLALTGVNQDDAGVASAVLNSTQQVGGSLGTALLNTFYASAVASYLVAHHL
                     PPSAFNPPAAIHGYHVAFWIGVGLIAAAFVAVLVLERVGKGVGAPSGRRVMLS"
     gene            1763..2584
                     /locus_tag="VF874_01920"
     CDS             1763..2584
                     /locus_tag="VF874_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004126090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX9174885.1"
                     /translation="MAGYPSDLTDRQWLVIESLVPPVRRGGRPAEHARRRIVDAILYV
                     VRTGCSWRQLPHDFPPWATVFWYFKQWRADGTVDRIHDALRDRVRDEAGRDPLASAGI
                     VDAQSVKGADTVGADSRGFDAGKKVNGRKRHIVVDTMGLLLAVIITTAAVQDRDGARR
                     VLDRLRFTMPSVALVWADGGYAGQLVIWARNRLRVLVEIVRKPLGIKTFHVLPRRWVV
                     ERTFAWMSRCRRLAHDYERLPEHSEAMIKWAMIGLMTRRLAPAPGHRPWQPSTIT"
BASE COUNT          414 a          906 c          894 g          502 t
ORIGIN      
        1 acggcgccaa ccagtgggcc ggggtgaaga cctacctcga cgcggtcacc gactacgccg
       61 ccgcgccggt catcgcgaag tacaccggcg tgctggcggc ggcggacgtg ttcaccacca
      121 gcaccacgct cgtcgtcttg gagtcgatcc gcctcgacct cggcgtcggc tcgcaggtcc
      181 acccgtgacc cgcatcggcg tcatcctcag gacaatcttg ctaggaaggc acgttcgatc
      241 atggcttcag acgcggagct gaccgcccaa ggccagcaag aggaccacga ggaccaactc
      301 gacgaacggc ccagccgccg atggtgggct ctggcggtca ttgcgatcgc tcaactaatg
      361 gtggtgctcg acggcaccgt cgtgaccatt gcgctgccct cggcgcaacg cgacctgggt
      421 atctccgacg ctgatcggca gtggacgatc accgcctaca ccctcgcgtt cggtggcctg
      481 ctgctgctgg gcggacggat cgccgactat cagggccgta agcggagctt cctggtcggg
      541 ctgctcgggt tcgcggccgc gtcggcgctc ggcggtgcgg cgcagaacgg cgcgacgctc
      601 ttcggcgccc gggccctcca gggcgcgttc gcggcgctgc tcgcgcccgc tgccttgtcg
      661 ctgatctcgg tgaccttcac cgaggtgagg gaccggtctc gggcgttcgc ggtctacggc
      721 gccatctcgg gaggcggcgc cgcgatcggc ttgatcctgg gcggcacact gaccgagtac
      781 gcgtcctggc gctggtgtct gctgatcaat gtgccgatcg cgattgtcgc cttcattgcc
      841 ggcacgatcc tgatcaccga gagcaagtcc gggggcggga gctcatatga catcccgggc
      901 gcgatcctcg gcacggccgg gctggtcgcc ctcgtgtacg gattcaccca agcggccaag
      961 accggtgtcg gctggctgtc ggtccaggcc atcagctgct tcgtgagcgc cggcatcttg
     1021 ctggtcgcgt tcgtgctcgt cgagttgcgt tcggcgagtc cgctgttgcc gatgcgggtg
     1081 gtgctcgagc gcaatcgcgg cggctcgtac ctcgcctcgc tgctggtctt cgccgggctg
     1141 ttcgcgatgt tcctgttcct gagttactac ttccagtaca acctgggtta cagcgcactc
     1201 aagtcgggta tcgcattcct gccgttcagc gccggcatca tcgtcacctc ggccgtggtg
     1261 tcgggcctgc taccgcgcac cggacccaaa ccactgatgc tgatcgggct cgcgatggtt
     1321 gcgaccggcc tggccacctt cacccgcatc tccgacacca gcagctgggt tagcaccgtt
     1381 ctgcccggag agctactgat gagcgtcggc ctggcgctgg tgatcgtgcc gctctccaac
     1441 ctcgcgctca ccggggtgaa ccaggacgac gcgggagtcg ccagcgcggt gctcaactcg
     1501 acccagcagg tcggcggatc cctgggcacg gcgctgctga acaccttcta tgcgagtgcg
     1561 gtcgcctcct atctcgtggc ccatcacctc ccaccgtccg cgttcaaccc gcccgccgcg
     1621 atccacggct atcacgtcgc gttctggatc ggggtcgggc tgatcgccgc ggcgttcgtg
     1681 gccgttctgg tcttagaacg tgttgggaaa ggtgtcggcg cgccttccgg acgacgggtc
     1741 atgttgagtt gatctttggt ggatggctgg gtatccgtct gatttgactg atcggcagtg
     1801 gttggtgatc gagtcgttgg tgccgccggt gcgccgtggc gggcgaccgg ccgagcacgc
     1861 ccgccgcagg atcgtggatg cgatcttgta tgtggtgcgg acgggatgct cgtggcggca
     1921 gctgccgcac gactttccac cgtgggcgac ggtgttctgg tacttcaagc agtggcgggc
     1981 cgacggtacg gtcgatcgga tccacgatgc gttgcgcgat cgggtacgcg acgaggccgg
     2041 ccgggatccg ctggccagcg cgggcattgt ggacgcccag tcggtcaagg gcgcggacac
     2101 cgtcggtgcc gactcgcgcg gcttcgacgc cggcaagaaa gtcaacggcc gcaagcgaca
     2161 catcgtggtc gacaccatgg gcctgctgct cgcggtgatc attaccaccg cggcggtgca
     2221 agaccgcgac ggcgcgcgcc gggtgctgga ccggctgcgg ttcaccatgc cctcggttgc
     2281 gttggtgtgg gccgacggcg gctacgccgg ccaactcgtc atctgggccc gcaatcggct
     2341 gcgcgtgctg gtcgagatcg tgcgcaaacc gctgggaatc aagaccttcc acgtgctgcc
     2401 ccgccgctgg gtcgtcgaac gcaccttcgc ctggatgagc cgatgccgtc gcctggccca
     2461 cgactacgaa cgccttcccg aacactccga agcgatgatc aaatgggcca tgatcgggct
     2521 gatgacccgc cgtctcgccc cggcacccgg acaccgacca tggcaaccgt caactatcac
     2581 ttgagacttt cccaacacgt tcttaggtaa tacaccctca ccaccggaaa aacccggccc
     2641 tgaaaggcca aacagctaaa cgatcaagaa aggccggaaa ctcccgctag gagtgccctg
     2701 aataatccgc gcggct
//
LOCUS       DASWBF010000068         8844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1253442,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000068 DASWBF010000000
VERSION     DASWBF010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253442"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..325
                     /locus_tag="VF874_01925"
                     /pseudo
     CDS             1..325
                     /locus_tag="VF874_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247720.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            372..815
                     /locus_tag="VF874_01930"
     CDS             372..815
                     /locus_tag="VF874_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9174886.1"
                     /translation="MQAEEIAEDFPVVSMGTSALDAARMLAEHRLPGIVVTDPSGKPV
                     AVLPASQVVRFIVPAYVQDDPSLAGVLNESMADRAAEKLGGKTVRDVLPEHLLNVPAA
                     DADDTIIEVAAIMARLRSPLIAVVKDGKLHGVITASRLLAAALKH"
     gene            823..2112
                     /locus_tag="VF874_01935"
     CDS             823..2112
                     /locus_tag="VF874_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX9174887.1"
                     /translation="MTVIAITVFVVGYALIASDRVNNSVVALTGAAVLVALGVVASDD
                     VFYSRDTGIDWDVIFLLLGMMIIVSVLRQTGVFEYAAIWSAKRAKGSPLRIMILLVLV
                     TAVASALLDNVTTVLLIAPVTLLVCDRLAIRAAPFLMAEVFASNIGGAATLVGDPPNI
                     IIASRAGLTFNDFLIHLAPIVVIVMVVFVALLPRLFRGSFGVDPQRVADVMSLEEREA
                     IQDTRLLIKCGVVLVLMFAAFVAHSTIHIEPSVVALLGAGVLVLISGLERSDYLSAVE
                     WDSLLFFAGLFIMVGALVKTGVIDQLAGLAREAAGGMALLTVMLILGVSAPVSGIVNT
                     IPFVATMAPIVSELAAGMHEPSHANALWWALTLGADFGGNLTAVGASANVIMLGIARR
                     ADNPLSFWEFTRKGAVVTAVSVALAAIYLWLRYFTFG"
     gene            2168..4069
                     /locus_tag="VF874_01940"
     CDS             2168..4069
                     /locus_tag="VF874_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX9174888.1"
                     /translation="MRAADTVRLALIVGALGVVFGDIGTSPIYTIQTVFNPSDPHPVP
                     ISTDNVYGVVSLIFWSVMIIVTLTYVTLVMRADNDGEGGIMALITLLRRWRGTRGRRT
                     AMTLAALGIFGAALFFGDSMITPAISVLSAVEGLKVVQPGLEQWIVPITALIIVALFA
                     VQRHGTAAVGKSFGPVMIVWFMAIGACGVYGIVDHPEILKALSPTYALKFIVGHFGIA
                     FFALAAVVLSVTGAEALYADMGHFGRRAITRGWLGLVLPACTLSYFGQGALLLGDQTA
                     VSAPFFLLTPEWARLPMVVLATAATVIASQAVITGAYSVASQAAQLGYLPRLRITHTS
                     ESTIGQIYVPWINGVLMVSVLTLVFAFRSSVALAFAFGMAVTGTITITTLLFFYVVRT
                     RWGTPLWLVLLGAGFLVLVDLMFFAANLTKLVHGAWLPLLIGLTTFTVMTTWQRGREI
                     VTRARAQAEGPLREFIGQLHGPQPLLLIIPGTAVFLNRGKETAPLAMRANVEHNRVIH
                     EHVVIMSIETLPVPRVPDAERVVVDDLGYARDGIIHVSANFGYMETPNVPDALRLLDP
                     AQTEGVIDLENASYFLSKLELQKGTAPTMAAWRKRLFIATSYITADAAEYFGLPLDRT
                     VIMGSRIEV"
     gene            complement(4066..4558)
                     /locus_tag="VF874_01945"
                     /pseudo
     CDS             complement(4066..4558)
                     /locus_tag="VF874_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098870.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            4512..5021
                     /locus_tag="VF874_01950"
                     /pseudo
     CDS             4512..5021
                     /locus_tag="VF874_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877325.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
     gene            complement(5251..6075)
                     /locus_tag="VF874_01955"
     CDS             complement(5251..6075)
                     /locus_tag="VF874_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9174889.1"
                     /translation="MSEPVSTSPTVLVTGATDGIGYATARRFVDDGATVYLHAPDQGS
                     GENAMTRLVKDGAEPLRLHLVVADFTRLDEVAGLADTLTATLSALDVLVNNAAVAGPE
                     RRTYTQDGHELTFQVNYLAPYLLTTKLVPRLASAGGRVVNVSSAMHRGGNINWNNPAG
                     KHQYWPLAAYAQSKLALTMYTKSLAEAGGASFTALSVHPGVFQTRLLPIYGRVGRPAE
                     EAAPILTTLGSPTHPVVDGGYYDGLSLAPAAALVENPRARSRLAKLSAHLVGSALH"
     gene            complement(6500..7297)
                     /locus_tag="VF874_01960"
     CDS             complement(6500..7297)
                     /locus_tag="VF874_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174890.1"
                     /translation="MGGIHTSSGPAEFGGDRVSVTAIVDHVVAVRRCSEWQHVLPDAE
                     IAFPARLLAVATTAQWVKDRADKGLCVGVRGDQDLDHALSAGVPPARMVFHCERAATG
                     TVWNAVGVGVGRFIVGTERQMILVSACAPERQSVLIDVMDRRQADDLLTTVLADEGLS
                     LTGLHVQLDNAIAADQVKYAIEQMARFSRKHAVVFSRLSLAVSEPLSAAPQARAALVA
                     AVDDAIDTSCRRLLLPRPALMVSPQWPTITGRGRSHPSAASQLPRPA"
     gene            7683..8510
                     /locus_tag="VF874_01965"
     CDS             7683..8510
                     /locus_tag="VF874_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9174891.1"
                     /translation="MSPNDAVGTVVVGVDGSKAAINAALWAIDEAISRDVPLRLIHVV
                     EPKAEGIGHRDEVLHAAVAAVKATGRSVRVEAIARRGNVETALVEESSVASMVCVGSA
                     GVGRVARRLLGSAAAVLATHAHCPVAVIRSVDDVLSTGDRIAVVVDAEPGNDAVVQYA
                     LEEARLRKAVLVALGVWRWDLGTMPYEELDQRLSAWLPRYPDVQVSLCAAGSGVVAYL
                     ADKDAHVGLVVIGDAGAAQVARLIGPHSHPLLAHPDCSVLVVHPSRRWRGGPQWFDD"
     gene            complement(8606..>8844)
                     /locus_tag="VF874_01970"
     CDS             complement(8606..>8844)
                     /locus_tag="VF874_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174892.1"
                     /translation="AQIPQAIAAASIMLTVYYWRRQGQRRSARNLGAVGNDRKQRGYD
                     KSHTGTSAPWLPSWARCGVLMGVLAVAAGASRRP"
BASE COUNT         1436 a         2847 c         2902 g         1659 t
ORIGIN      
        1 gaccgggtca ccaagctgct gatcagttcg acggcgccga acaccaccgc tgcgccggtt
       61 ggtatcgcgc gcgttcaccc cgcgcaacgc cttacggctg cgcgccgcgt gcgtcgacgt
      121 gatcaccgag ctgatcgagg cgcacaccgc cgcgggccgg tgcgacatca tcgccgacat
      181 cgcccggcca tacccggtgc cgatcatctg cgcgctgctc ggcgtacccc gcaaggactg
      241 gcacctgttt tcccactggg ccaatgacat cagcacggcc ttcggctgca acgtcgccga
      301 ggaagcgcct gccatcctgc gggcgtgaga gcaacgttgg cggacgcggg tcgcggtcgt
      361 atcgtgcagt tgtgcaggca gaggaaattg ccgaggattt tccggtcgta tcgatgggca
      421 ccagtgccct cgacgccgcc cgcatgctgg ccgagcaccg gctgcccggg atcgtcgtca
      481 ccgacccgtc cggcaagccg gtcgccgttc tgccggcctc ccaagtggtc cgtttcatcg
      541 tgcccgcgta tgtgcaagac gatccgtcgc tggccggggt gctcaacgag tcgatggccg
      601 atcgggccgc cgagaaactg ggcggcaaga ccgtccgcga cgtgctgccg gagcacctgc
      661 tcaacgtgcc cgccgccgac gccgacgaca cgatcatcga ggtggccgcg atcatggcgc
      721 ggctgagaag tccactgata gctgtcgtga aggatggcaa actgcacggc gtgatcaccg
      781 catctcgcct actggctgca gcactgaagc actgacgggc tcatgaccgt catcgcgatc
      841 acggttttcg tggtcgggta cgcgctgatc gccagcgatc gtgtcaataa cagcgttgtc
      901 gcgctcaccg gagcggcggt cctggtcgcc ctcggcgtcg tcgcatccga tgacgtcttc
      961 tactcccgcg acaccggaat cgactgggac gtcatcttcc tgctgctcgg gatgatgatc
     1021 atcgtcagcg tcctgcgcca gaccggtgtg ttcgaatacg ccgcgatctg gtcggccaag
     1081 cgcgccaagg gctcgccact gcggatcatg atcctgctgg tgctcgtcac ggcggtcgcc
     1141 tcggcgctac tcgacaacgt caccacggta ctgctgattg ctccggtcac gctgctggtc
     1201 tgtgaccggc tcgccatcag ggcggcgccg ttcctgatgg ccgaagtgtt cgcatccaac
     1261 atcggcggcg ccgcgacgct ggtcggcgac cccccgaaca tcatcatcgc gagccgagca
     1321 ggcttgacgt tcaacgactt tctcatccac ctggccccga tcgtcgtcat cgtcatggtg
     1381 gtgttcgtcg cgctgctgcc acggctgttc cggggctcct tcggggtcga tccccagcgc
     1441 gtcgcagatg tgatgtcact ggaggagcgc gaagccattc aggacaccag gctgctgatc
     1501 aagtgcggcg tcgtcctcgt cctcatgttc gccgcgttcg tcgcgcactc cacgatccac
     1561 atcgaacctt cggtggtcgc gctgctcggg gccggtgtgc tggttctgat ctcggggttg
     1621 gagcgatcgg actacctctc cgcggtcgaa tgggactcgc tgctgttctt cgccgggctg
     1681 ttcatcatgg tgggcgcgct ggtgaagaca ggtgtcatcg atcaactcgc cgggctggcc
     1741 agggaagcgg ccggcgggat ggcgctgctg acggtgatgc tgatcctcgg tgtgtcggcg
     1801 ccggtctccg gcatcgtgaa cacgatcccg ttcgtcgcca cgatggcgcc gatcgtcagc
     1861 gagctggccg ccggcatgca cgaaccgtcg cacgctaatg cattgtggtg ggccctgacg
     1921 ctcggcgccg acttcggcgg caacctcacc gccgtcgggg ccagtgccaa cgtcatcatg
     1981 ctggggatcg cccgccgcgc cgacaacccc ttgtccttct gggagttcac ccgcaagggc
     2041 gcggtggtca ccgcggtatc cgtcgcgctc gcggcgatct atctgtggct gcgctacttc
     2101 acttttggct gatgaggagg acctcgacgt gcagacagac tcccaacccg cgacgccgtt
     2161 tcccaccatc cgcgctgcgg acacggttcg cttggcgctc atcgtcggcg cactcggcgt
     2221 cgtgttcggc gacatcggga ccagcccgat ctacaccatc cagacggttt tcaaccccag
     2281 cgatccgcac ccggtgccga tcagcacgga caacgtgtat ggggtcgtgt cgctgatctt
     2341 ctggtcggtg atgatcatcg tcacgctgac gtacgtcacg ctggtgatgc gcgccgacaa
     2401 cgacggcgag ggcggcatta tggcgctcat cacgctgctg cggcgttggc gcggcacgcg
     2461 gggacgtcgc accgcgatga cgttggctgc gctcgggatc ttcggcgccg cactgttttt
     2521 cggcgacagc atgatcaccc ccgcgatctc ggtgctgtcc gcggtggagg gcctcaaggt
     2581 ggtccagcct ggcctggaac agtggatcgt gccgatcacg gcgctgatca tcgtggcgct
     2641 gttcgccgtg caacgccacg ggacggccgc tgtcggtaag tctttcgggc cggtgatgat
     2701 cgtctggttc atggcgatcg gcgcgtgcgg cgtgtacggc atcgtcgacc acccggagat
     2761 cctcaaggcg ctctcgccta cctatgcgct gaagttcatc gtcggacact tcggcatcgc
     2821 tttcttcgcc cttgccgccg tcgtgctatc ggtgaccggt gccgaggcgc tctacgccga
     2881 catgggccac ttcgggcgcc gggcgatcac ccgcgggtgg ctcggcctgg tgctgccggc
     2941 ctgcacactg agctatttcg gccaaggcgc gctgctgctc ggcgaccaga ccgcagtcag
     3001 cgccccgttc ttcctgctca cccccgaatg ggcgcggctg ccgatggtgg tgctggccac
     3061 tgccgccacc gtgatcgcgt cccaggcggt gatcaccggt gcatactccg tggcgtcgca
     3121 ggccgctcag ctcggctatc tgccgcgact gcggataaca cacacctcgg agtcgacgat
     3181 cggccagatc tacgtgccgt ggatcaacgg tgtgctgatg gtgtcggtgc tcaccctggt
     3241 gttcgcgttc cgcagctcgg tggctttggc attcgcgttc gggatggcgg tgaccggcac
     3301 gatcaccatc acgacattgc tgttcttcta cgtcgtgcga acccggtggg gcaccccgct
     3361 ttggctggtt ctgctggggg ccggattcct ggtgctggtc gacctgatgt tcttcgcggc
     3421 gaacctcaca aagctcgtac acggagcatg gctgccgctt ctcatcggcc tcaccacctt
     3481 cacggtgatg accacctggc agcgtgggcg cgagatcgtc actcgcgcac gggcccaagc
     3541 ggagggaccg ctgcgcgagt tcatcggcca gctccatggc cctcagccgc tgctgctgat
     3601 aatcccgggc accgcggttt tcctcaaccg cggtaaagag actgcgccgc tggcaatgcg
     3661 ggccaacgtc gagcacaatc gcgtaatcca tgaacacgtg gtcatcatgt ccatcgaaac
     3721 cctgccggtg ccgcgcgtgc cagacgccga gcgggttgtg gtcgacgatc tcggctacgc
     3781 cagggacgga atcatccacg tatcagccaa cttcgggtat atggaaacgc cgaacgtgcc
     3841 cgacgcgctg cgcctgctcg atccggcgca aaccgaaggc gtgattgact tggaaaacgc
     3901 ttcgtacttc ctgtcgaagc tcgagctgca aaagggtacg gcaccgacca tggcggcatg
     3961 gcgcaagcgc ctgttcatcg cgacctctta catcaccgcc gacgcggccg agtatttcgg
     4021 cctgcctctc gaccgtacgg tgatcatggg ctctcggatt gaggtctagc ggccgttctc
     4081 tgctagcccc agcgcagcca gtgcggcgtc gaccgccgcc gcccgcggcc gcttgaccgg
     4141 ggtatcgggg aactgaggca gcaatcctga agaccgccga atgcgaccac cgcgcgcgtt
     4201 gcctgcagca gcgtcggcct cgggccgaac accagcttgg cgagtcgttc ccgccaggtc
     4261 agcaccttgt cgaccagccc aaggtcctgg ccagcatgcc catctcgacc agcaccagca
     4321 ttaggtccct tcgatgccgg tagtggaaat cgaagtaact ctcgagcagc gcactcacgg
     4381 gcgtgtcacg gcgtctctcg tgctccagga caagcttctc accgtcgtcg atcaacggct
     4441 ggacgatgct gcggacgagg tcgtcccgtg acgaaaagtg atcgtacaca gcggctttgg
     4501 tgattccaag catgtcggcg atatcctgca ggctggtgcg ctgcactccc gtcctcattt
     4561 cgggggccag tattgccggg ccggtactgg tgtactggct cacgcggtat ggcttcgatg
     4621 tgacggtgtc gtcgagcgcg cgccggcgct ccgaaagacg ggcggccacg ccgtcgattt
     4681 gttccggccg gcgtcggaga tctcggagaa gatgggcgtg ctgccgcgga tcgaggcgct
     4741 cgccacgggc acgacccgga tgaccgtgca ccgcgagggc gtgaagcggc ctctcagagt
     4801 cgatctcacg aaggtttacg cagccgcctc agaccggcat gtggagatca tgcgcgacga
     4861 tctcagcgag atctactacg ccggccgtga cgacgtcgag tatctgtcgg tgattcgatc
     4921 accgccacct ccccgacggc gatgtcgctt tcgagcgcgc agcgccacgc cagttcgaca
     4981 tcgtcgtcgg cgccgacggt ctgcatgcga acgtgaggcg tacaccaagg gcctgcgcct
     5041 acacgattcg atgcgggtga aggactacgc catcgggccg gtcggctagc gcgggatccg
     5101 cagggtcggt gatgtcgtcg tatgtgggct ttccatgcgt tatgttcccg cgcgttttcc
     5161 agaggctggc cgttttccgt ggttggcctt cttctttcga cgagcgcgct tgtcggctcg
     5221 tttggacatc gcgtccgcct cctttctttg tcagtgcagc gctgagccga cgaggtgagc
     5281 gctgagtttc gccaggcgac tgcgggcgcg cgggttctcg acgagcgccg cggccggggc
     5341 gaggctcagg ccgtcgtagt agccaccgtc gaccacggga tgggtgggtg acccgagcgt
     5401 ggtcaggatc ggagcggcct cctcggccgg tcggccgacg cggccataga tgggcagcag
     5461 ccgggtctgg aacacgccgg gatggacgct gagcgcggtg aacgaagcgc cgccggcctc
     5521 ggccagtgac ttggtgtaca tcgtgagggc gagcttggat tgtgcgtagg ccgccagcgg
     5581 ccagtactgg tgcttaccgg ccgggttatt ccagttgatg tttccgccgc ggtgcatcgc
     5641 cgaggacacg ttgacgactc gtccgcccgc gctggcgagc cggggcacca gtttcgtggt
     5701 tagcagatac ggcgcgaggt aattgacctg gaaggtcagc tcgtggccgt cctgcgtgta
     5761 ggtgcgtcgc tcgggtccag cgactgcggc gttgttgacc agcacatcca gcgccgacag
     5821 cgtcgcggtc agcgtgtcgg cgaggccggc gacctcatcc aggcgggtga agtcggcgac
     5881 gaccagatgc aggcgcagcg gttctgcgcc atctttgacc aggcgcgtca tcgcgttctc
     5941 accgctgccc tggtccgggg cgtgcagata cacggtcgcg ccgtcatcga caaaccggcg
     6001 cgccgtcgca taaccgatgc cgtcggtggc gccggtgacg agaaccgtag gagaggtgga
     6061 aacaggttcg gacataatag ctttcgcgga attgaaggta cgaccgcgga catcgcctga
     6121 cacgacgcgg cgtttgaatc gaatagaaat agggagcgag tcggaaacag gtctgaaccg
     6181 tcgagcgcga aacagctctg ttcaggcgct gattcgcgcg cgcgacggcg gttcgtcctc
     6241 ccagcccaca ccgtaacgct cgagcgccga aggcactgcg agggcaacta cagtggtgtg
     6301 ggtcatgcgt tcgattgcag caggccgcga agcgcgggca acgcaactta cgacttcttg
     6361 acgcgatgac cgcttttctt gatggtctct tgacacggcc aggcacaccg tctcggccga
     6421 gattctttat gccacagcct acttgccgca tgagcagatc gtgcttgtcg ctcacccaaa
     6481 ggaaaagccc caactggtgc tacgccgggc ggggcagctg actagcagct gatgggtgtg
     6541 atcggcctcg tccggtgatc gtcggccact gtggcgacac catgagcgcc ggacggggca
     6601 acagcagccg ccgacagctg gtgtcgatcg catcgtcaac ggcggcgact aaagcggctc
     6661 tggcctgcgg tgcggcgctg agcggctccg ataccgccag gctgagtcga gaaaacacca
     6721 ccgcgtgctt gcgcgagaac cgcgccattt gctcaatcgc gtatttgact tggtcggccg
     6781 caatggcgtt gtccagctgc acgtgcagcc cggtcagcga tagtccttcg tcggcgagga
     6841 ccgtagtcaa cagatcgtcg gcctgccgcc gatccatgac gtcaatcagc acggactggc
     6901 gttccggcgc acacgccgac accaggatca tctgccgctc ggtacccaca atgaagcgcc
     6961 cgacgcccac gccaacggca ttccacaccg tgccggtagc cgcgcgctcg cagtgaaaca
     7021 ccatgcgggc gggagggacg ccggccgata acgcgtggtc gaggtcctga tcgcctcgca
     7081 cgccgacgca caggcccttg tctgccctgt ccttgaccca ttgcgctgtg gtggcaaccg
     7141 ccagtaatcg tgccggaaag gcgatctcgg cgtcgggcaa cacgtgttgc cactctgagc
     7201 aacgacgtac cgctacgacg tggtcgacga tcgccgtaac tgagacccga tcgccgccga
     7261 attccgccgg cccggacgat gtgtggattc cgcccataca tcagaactac aacgtgatga
     7321 gaccctgcgg gtagggctgc taacgcgccc ttaacgggtc gggatacgaa ccttgacgaa
     7381 cctctgatac cgccccaata gcggctatga gataccgcga tgggctgact atgtcacaca
     7441 agcagcccag ccatgggatg gccgctggtc gcacacctgg gtgtgctctt ctggatggtg
     7501 cttggtggtc gccgcgacgc gagccgcggg gccggtgggg tcgagcggtc gcctccggca
     7561 gataacgaac agcgtcgtag atggggtcta acgactcctg gcgagtgttc agccggccga
     7621 tagcgtgggt gccatatgtc gtgcagtccc gatgtgtgac gatcaactca ggagtgtgtg
     7681 cgatgtcgcc taatgatgcg gtcggcacgg ttgtggttgg tgtcgacggc tcgaaggcag
     7741 ccatcaacgc cgccttatgg gcgatcgacg aagcgatcag tcgtgatgtg ccgctgcgcc
     7801 tgatacatgt cgtcgagccc aaggccgagg ggatcggtca tcgcgacgag gtcctgcatg
     7861 cggcagtcgc tgcggtcaag gccaccggcc gatcggtaag agtcgaagcc atcgctcgcc
     7921 ggggcaacgt cgagacagcg ttggtcgagg aatcgagtgt cgcgtcgatg gtctgtgtcg
     7981 gatcggcagg ggttggacgc gtggcccgca ggttgttggg ctcggccgcg gccgtattgg
     8041 ctacccacgc gcattgcccg gtggcggtga ttcgcagcgt tgacgatgtg ctctcgaccg
     8101 gtgaccgcat cgcggtggtc gtcgatgccg aacccggcaa tgatgcagtc gtgcagtacg
     8161 ccttggagga ggcgaggctg cgtaaggcgg ttctggtggc gctgggtgtg tggcgctggg
     8221 acctcgggac gatgccctat gaagagctcg atcagcgact cagcgcgtgg ctccctcgtt
     8281 acccggacgt gcaggtatcg ctgtgcgccg cgggcagtgg cgtggtcgcg tatctggcgg
     8341 ataaggacgc ccacgttggt ttggtggtca tcggtgacgc cggcgccgct caagtggcgc
     8401 gtctcattgg cccacactcc cacccgctac tcgcgcaccc cgactgctca gtactggtgg
     8461 tgcatccaag ccgccgatgg cgcggcggcc ctcaatggtt cgacgactag gcgggcgacc
     8521 gacacccggt ttaagccgta ccgtacaacg tcaaatccgc cgatcgcgga ctacgacacg
     8581 cgcgtcgcgc cccaccggac tcggtctatg gccggcggga ggcgccggcg gccaccgcca
     8641 gtacgcccat gagcacgcca caccgcgccc acgacggcaa ccatggtgcg gaagtccctg
     8701 tatgactttt gtcgtagccc cgctgcttgc ggtcattgcc gaccgcgcca agattccgcg
     8761 cggagcgccg ttgtccctgc cgacgccagt agtagacggt cagcatgatc gatgccgcgg
     8821 cgatcgcctg gggaatctgc gcca
//
LOCUS       DASWBF010000069         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_401662,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000069 DASWBF010000000
VERSION     DASWBF010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401662"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VF874_01975"
     CDS             <1..1531
                     /locus_tag="VF874_01975"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX9174893.1"
                     /translation="RLHGSAIADPTLAGAAWLNRESPVSQALLTQLYAGFTERYADRI
                     AARHRDVCDRLIASFDAYAAPVGPQGLVHGDCRLDNMLFGTEDADRPLTVVDWQTVTW
                     GPVMTDLAYFTGCALTVPVRRAHADELVACYHTALGPTTLSLDDVRDGVRRQSFFGVM
                     MSIVSPMLVQRTERGDDMFMAMIERHCEQVLDTDALALLPEPVAPQPLTPDPADEFAH
                     PAGAEPMWNESWYCDFVDPRQRVGGYVRLGLTPNEGVAWFTALLCGPDQPTTAVLDYA
                     APLGDPYTLNTNAFDFAHAVVSPLQRYRLVLRARGEAHDDPAALLRAEHGRGVDVSMD
                     LEWVTVGTPYQYRLTPRYEIPCAVTGSISVDGRPMRFEAVPGQRDHSWGVRDWWGMDW
                     VWSALHLDDGTHLHGVDIRLPGAPPIGVGYSQRPGAALTELQAVTARASFADNGLPVT
                     TSITFDQLEAVADIRGHAPLRLAAPDGRVAWFARAWASITTSDNREGIGWIEWNRNNF
                     E"
     gene            1528..>1813
                     /locus_tag="VF874_01980"
     CDS             1528..>1813
                     /locus_tag="VF874_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride ion channel protein"
                     /protein_id="HEX9174894.1"
                     /translation="MTRTQRYLTFAAAILVTGVLSGIAGGFTTLLVKAIEHLTYGYAQ
                     GPLLTGVKDASIERRLLGPAIGCALAGAGWWLLRRKATIPNLTEQIRSGRP"
BASE COUNT          297 a          632 c          591 g          293 t
ORIGIN      
        1 ccgcctgcac ggatccgcca tcgctgaccc gacattggcc ggcgcggcgt ggctcaaccg
       61 cgagtcgccg gtcagccaag cattgctgac ccagctctac gccggattca ctgagcggta
      121 tgcagatcgg atcgcggcgc gacaccgcga cgtgtgcgac cgcctgatcg ccagctttga
      181 cgcgtacgcc gcgcccgtgg gcccgcaggg ccttgtgcac ggcgactgtc gactcgacaa
      241 tatgctgttc ggcacagagg acgcggaccg accgctgacc gtcgtcgact ggcagaccgt
      301 cacctggggt ccggtgatga ccgatctcgc ctatttcacg ggctgcgcgc taacggtgcc
      361 tgttcgtcgc gcgcacgccg acgagctcgt cgcgtgctac cacaccgcat tgggtcccac
      421 gacgctatcg ctcgacgacg tccgcgacgg tgtgcgccgg caaagctttt tcggagtgat
      481 gatgtcgatc gtctcgccga tgctcgtgca gcgcaccgaa cgcggcgacg acatgttcat
      541 ggcgatgatc gaacgacact gcgagcaggt gctcgacacc gacgcattgg ctctgctgcc
      601 cgaacccgtt gcgccgcaac cgctcacgcc ggacccggcc gacgagttcg cgcacccggc
      661 cggcgccgag ccgatgtgga acgaaagctg gtattgcgat ttcgtcgacc cacgccagcg
      721 tgtcggcgga tacgtccggc tgggcctgac gcccaacgaa ggcgtcgcct ggttcaccgc
      781 gttgctgtgc ggccccgacc agccgacgac cgcggtgctc gattacgcgg ccccgctcgg
      841 cgacccgtac accttgaaca ccaacgcatt cgacttcgct catgcggtcg tgtcgccgct
      901 gcagaggtac cgtttggttc tgcgtgcccg cggcgaagcg cacgacgatc ccgccgcgct
      961 gctgcgcgca gaacacggcc gcggtgtcga cgtcagcatg gacctggagt gggtgaccgt
     1021 cgggaccccc tatcaatacc gcctgacacc acgctacgaa atcccctgtg cagtaacagg
     1081 aagcatcagc gtcgacggcc gaccgatgag attcgaggcg gtgcccggcc agagagacca
     1141 ctcgtggggc gtccgcgact ggtggggcat ggactgggtg tggagtgcgt tgcacctcga
     1201 cgacggcacg cacctgcacg gcgtcgacat ccgactgccc ggcgcaccgc cgatcggggt
     1261 cggctactcc cagcgcccgg gcgctgcgct gaccgagctg caggccgtca ccgcacgagc
     1321 atcgttcgcg gacaacggat taccggtgac cacctcgatc accttcgacc agctggaggc
     1381 tgtcgccgac atccgcgggc acgccccgct gcggctggcg gcacccgacg gcagggtggc
     1441 atggttcgcg cgggcgtggg cgtcgatcac cacatcggat aaccgcgagg gtatcggctg
     1501 gatcgagtgg aaccgtaaca atttcgaatg acccgcacgc agcgctacct caccttcgcg
     1561 gcggccattc tggtcaccgg cgtcctcagc ggcatcgccg gtggtttcac cacgctgctg
     1621 gtgaaggcga tcgagcacct gacttacggc tacgcgcagg gaccgctgct caccggtgtc
     1681 aaagacgcct cgatcgagcg gcggctgctc ggcccggcca tcggatgcgc gctggccggt
     1741 gcagggtggt ggctgctccg ccgcaaggcg acgatcccga acctcaccga gcagatccgg
     1801 tctggccgtc cat
//
LOCUS       DASWBF010000070         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1191016,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000070 DASWBF010000000
VERSION     DASWBF010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191016"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1526
                     /locus_tag="VF874_01985"
     CDS             39..1526
                     /locus_tag="VF874_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9174895.1"
                     /translation="MTTDIPATVRAGDVEEWSDDVDVVVIGFGIAGGCAAVSAAAAGA
                     RVLVLERAAAAGGTTAMAGGHFYLGGGTAVQQATGHPDSPEEMYKYLVAVSREPEHDK
                     IRAYCEGSVEHFNWLEDLGFQFERSFYPGKVVVPPGTEGLSYTGNEKVWPFCRQAKPA
                     PRGHSVPVPGELGGAAMVIELLVKRADELGVQIRYETGATGLILDEDGAVVGVAWKHY
                     AETGALKANSVIIAAGGFAMNPEMVAKYTPALGQQRRTKHHGVVAPYILGNPYDDGLG
                     IRMGVSAGGVAKNLDQMFITAAAYPPEILLTGVIVNKEGKRFVAEDSYHSRTSAFVLE
                     QPDQTAFLIVDEAHMEMPEMPLIKFIDGYETVAEIETALGIPAGELAATLDRYNEYAA
                     RGEDPDFHKQPEYLAAQDKGPWAAFDLSLGRAMYSGFTMGGLAVSIDGEVLREDGSVI
                     PGLYAAGACASNIAQDGKGYASGTQLGEGSFFGRRAGAHAAKRRR"
     gene            complement(1523..>2418)
                     /locus_tag="VF874_01990"
     CDS             complement(1523..>2418)
                     /locus_tag="VF874_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9174896.1"
                     /translation="SQASVVLPWVIQAPRLFAGEIKFGDVGQTASAFGKISESLSFFR
                     NSYDAFAVLRAAIIRLDGLVDANERARQLPTIDTEDTATDFVELDDVEVRTPDGVQLI
                     SPLDLHLDPGDGLVITGVSGTGKTTLLRSLAQLWPFATGTLRRPGDENETMFLSQLPY
                     VPLGDLRAVVSYPAAAGDIPDRQLQKVLVKVALPNLVDRLDEVQDWAKVLSPGEQQRV
                     AFARILLTKPKAVFLDEATSALDVGLEMTMYQLVRTELPRCIVVSVSHRPAVEQHHEK
                     HLQLLGDGRWELGRVEQTEPV"
BASE COUNT          447 a          769 c          810 g          392 t
ORIGIN      
        1 atccgggcgt tcttcgacta cgtgaagcgg gaggcgacgt gacgaccgat atcccagcga
       61 ccgtgcgcgc cggcgacgtc gaggaatggt ccgacgatgt cgatgtggtg gtgatcggct
      121 tcggcatcgc cggcgggtgc gcggcggtca gcgctgcggc ggcgggagcc cgggtgctgg
      181 tgctggagcg agcggcggcg gccggcggaa ccacagcgat ggccggcggg catttctacc
      241 tcggcggcgg caccgcggtg cagcaggcga ccggccatcc cgattcgccg gaggagatgt
      301 acaagtacct cgtcgcggtg tcgcgcgaac ccgagcacga caagatccgc gcgtactgcg
      361 agggcagcgt cgaacacttc aactggttgg aggacttggg ctttcagttc gagcgcagct
      421 tctacccggg caaggtcgtc gttccgcccg gcaccgaagg cctgtcgtac acgggcaacg
      481 agaaggtgtg gccgttttgc cggcaggcga agccagcgcc gcgcgggcac tccgtgcccg
      541 tgcccggtga gctgggtggc gccgcgatgg tcatcgagct cctggtgaag cgggccgacg
      601 aactcggggt gcagatccgc tatgaaacag gagcgaccgg cctcatcctg gatgaggacg
      661 gcgcagtggt cggtgtcgcg tggaagcact acgccgaaac cggtgcgctg aaagcgaatt
      721 cggtgatcat cgccgccggc ggattcgcaa tgaacccgga aatggtcgcc aagtacacac
      781 ccgcgttggg ccagcaacgc cggaccaaac accacggcgt ggtggcgccc tacatcctgg
      841 gtaaccccta tgacgacggt ctgggcatcc gaatgggcgt gtctgcaggc ggagtcgcaa
      901 agaatctaga ccagatgttc atcaccgctg cggcctaccc tcccgagatt ctgctcactg
      961 gtgtcatcgt caacaaagag gggaagcggt tcgtcgccga ggactcatac cactcgcgaa
     1021 cgtcggcttt cgtcctcgaa caacccgacc agacggcgtt cctgatcgtc gatgaagccc
     1081 acatggaaat gccggagatg ccactgatca agttcatcga cggctatgag actgtggccg
     1141 agatagagac cgcgctcggg attcccgcgg gcgagctcgc ggccaccctc gaccgctaca
     1201 acgagtacgc ggcccgcggc gaagaccccg acttccacaa gcagcccgaa taccttgccg
     1261 cacaagacaa aggtccgtgg gctgcatttg acctctcgct cggcagggca atgtactcag
     1321 gcttcacgat gggcggtctc gcggtgtcca tcgacggtga agtactgcgg gaggacggca
     1381 gcgtcatccc cggcctctac gccgcgggcg cgtgcgcgtc gaacatcgcg caggacggca
     1441 agggctacgc cagcggaaca cagctggggg agggttcgtt cttcggccgc cgcgccggag
     1501 cgcacgctgc gaagcggcgg cgttagaccg gttcggtctg ctcgacgcgg ccgagttccc
     1561 aacggccgtc accgagaagc tgcagatgct tctcgtgatg ctgttccacc gccgggcgat
     1621 ggctgacgct gacgacgatg caccgcggca actcggtgcg caccagttga tacatcgtca
     1681 tctcgagacc cacgtcgagc gccgacgtcg cctcgtcgag aaacaccgct ttgggcttgg
     1741 tcagcagaat gcgggcgaac gccacccgct gttgctcgcc gggcgagagc accttcgccc
     1801 agtcctgcac ttcgtcgagg cgatccacaa ggttcggcag ggcaaccttg acgagcacct
     1861 tctgcagctg acggtccgga atgtcaccgg ccgctgccgg atacgacacg accgcgcgca
     1921 ggtcgcccag cggcacgtac ggcaactggg acaagaacat cgtctcgttc tcgtccccgg
     1981 gtcggcgtag tgtgcccgtc gcaaacggcc acagctgcgc caggctgcgc aacagcgtgg
     2041 tcttgccggt gcccgaaaca ccggtgatca ccaggccgtc gccagggtcg aggtgcaggt
     2101 ccagtggcga aatcagttgc acgccatcgg gagtgcggac ctcgacgtcg tcgagttcga
     2161 cgaaatcggt tgcggtgtcc tcggtgtcga tcgtgggcag ctgccgcgcc cgttcgttgg
     2221 cgtcgaccag tccgtcgagc cggataatgg ccgcacgtaa caccgcgaac gcgtcgtagg
     2281 agttgcggaa gaacgacagc gattcggaga ttttgccgaa tgcgctcgcg gtctgaccga
     2341 catcaccgaa cttgatctcc ccggcgaaca agcggggcgc ctgaatcacc cacggcagca
     2401 ctacgctcgc ctggctga
//
LOCUS       DASWBF010000071         3717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_728900,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000071 DASWBF010000000
VERSION     DASWBF010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728900"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..744
                     /locus_tag="VF874_01995"
     CDS             220..744
                     /locus_tag="VF874_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9174897.1"
                     /translation="MPNSGGRIGVQAVQLRAATSLNSQAIAHLDDLSKAIIEKLQEDG
                     RRSYAGIGKAIGLSEAAVRQRVQRLIDAGVMQIVAVTDPMQLGFARQAMIAMRCTGDT
                     IKLAEKLAELPSIDYLVLTAGSFDVIAEVVCEDDNDLLELLNSQIRALPGVLSTETLV
                     YLKLVKQQYNWGTR"
     gene            741..2147
                     /locus_tag="VF874_02000"
     CDS             741..2147
                     /locus_tag="VF874_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9174898.1"
                     /translation="MTTTDVKGAAGSLAAKADRHLWGHFARHGAGITPPIITRGEGVR
                     IYDDRGKCYLDGLSGLFVVQVGHGRKELAEVAAKQAEKLAFFPLWSYATPPAIELAAR
                     LAHYAPGDLNRVFFTTGGGEAVESAWKLAKQYFKLTGKPGKYKAISRSIAYHGTPHGA
                     LAITGLPTFKAPFEPITPGGFRVPNTNFYRALAPYDTDLKAFGQWAANRIAEAIEFEG
                     PDSVAAVFLEPVQNAGGCFPPPPGYFERVREICDEYDVLLVSDEVICAFGRIGSMFAC
                     DDFGYVPDIITCAKGMTSGYSPIGAMIASDRLFEPFSDGKTTFTHGYTFGGHPVSAAV
                     ALANLDVFEREGLNDHVKQTAPAFRGTLEKLLDLPIVGDVRGEGFFYGIELVKDKATK
                     QVLDDELSEWLLRSFLSTELFEAGLYCRADDRGDSVIQLAPPLISGQAEFDEIEQILR
                     GVLTEAWARLQNHGEPWH"
     gene            2278..3507
                     /locus_tag="VF874_02005"
     CDS             2278..3507
                     /locus_tag="VF874_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9174899.1"
                     /translation="MGGIRCAACRVLGAVSAAVLVGFPTLGAVPVASAEPVASPDTAS
                     CPYQLATPPAVDESEVPVAGDPPLPLPVPAKPAGGDGLSGCGVVVAPNTPPVPDDISA
                     EAWLVADLDTGDVIAAKDPHGRHRPASIIKVLVAMQAINEVGVGKVVTGTADDGNAEG
                     TRVGVNEGGTYTVYQLLHGLLMHSGNDAAHALAMQLGGMDVALDKINTLARKLGATDT
                     RAATPSGLDGPGMSTSAYDMGLFYRYAWQNPIFAQIVATRTFEFPDSHGGAPYPVEND
                     NQLLYNYPGALGGKTGYTDDAGQTFVGAASRDGRRLVVVLLHGTRQPIPPWQQAAHLL
                     DYGFATPLGTKVGKLVDPDPSLVPKPSDSPVSAAGPGLPGAMNELPVRVGVAIVGAMI
                     VFGLILAARSVNRRTQN"
BASE COUNT          632 a         1246 c         1229 g          610 t
ORIGIN      
        1 agcccgccgc gtctccacga cgaaccgggg cctgctaccc ggcgttccgg tacctaccgg
       61 gacgggactt ccacccgcta gcctgatcca gctttctgga cgcaccatgg cgaaacgtta
      121 gccgagcctc tacgcatccg gtagtttcag cggcgctttg caagcgaatg cattgtcata
      181 gttgcttacg acgacgaaat ccgtgcaaga atcgtgtcca tgcctaattc cgggggacgg
      241 atcggcgttc aggcggtgca gttgcgagcg gccacctcac tgaactccca agccatcgcg
      301 catctcgacg acctgtcgaa ggcgatcatc gagaaactgc aggaagacgg ccggcggtcc
      361 tatgccggaa tcggcaaggc gatcgggctg tcggaggcgg cggtgcgcca gcgcgtgcag
      421 cggctgatcg acgccggggt gatgcagatc gtcgcggtca ccgacccgat gcagctgggc
      481 ttcgcccgcc aggcgatgat cgcgatgcgc tgcaccggcg acacgatcaa gctcgccgag
      541 aagttggcgg agctgccgtc gattgactac ctggtgttga ccgcggggtc gttcgacgtg
      601 atcgccgagg tggtctgcga ggacgacaac gatctgctgg agctgctcaa ctcccagatc
      661 cgcgcactgc ccggcgtcct gtccaccgag accctcgttt acctcaaact cgtcaagcag
      721 caatacaact ggggcacccg atgacaacta ccgatgtgaa aggcgccgcg ggcagcttgg
      781 ccgcgaaggc cgaccgccac ctgtggggtc atttcgcgcg gcacggcgcg ggcatcactc
      841 cgccgatcat cacccgcggt gagggtgtgc ggatctacga cgaccgcggc aagtgctacc
      901 tcgacggcct gtcgggcctg ttcgtggtgc aggtcggtca cggccgcaag gagctcgccg
      961 aggtcgccgc caagcaggcg gaaaagctcg cgttcttccc actgtggtcg tatgccaccc
     1021 cgcccgcgat cgaactggcc gcacgactcg cgcattacgc acccggagac ctcaaccggg
     1081 tcttcttcac caccggcggc ggcgaagccg tcgagtcggc ctggaagctg gcgaagcagt
     1141 acttcaaact gaccggcaaa cccggtaagt acaaggcgat ttcgcggtcc atcgcctacc
     1201 acggcacccc tcacggcgcg ctggccatca ctggtctgcc gacgttcaag gcgccgttcg
     1261 agccgatcac gcccggcggg ttccgggtgc ccaacaccaa cttctatcgg gcgctggcgc
     1321 cctacgacac cgacttgaag gcgttcgggc agtgggcggc caaccggatc gccgaggcga
     1381 tcgaattcga gggacccgac tccgtcgcgg cggtgttcct ggagccggtg cagaacgccg
     1441 ggggctgctt tccgccgccg cccggctatt tcgagcgcgt ccgcgagatc tgcgacgaat
     1501 acgacgtgct gctggtctcc gatgaggtga tctgcgcgtt cggccggatc ggttcgatgt
     1561 tcgcctgcga cgacttcggt tacgtgccag acatcatcac ctgcgcgaag ggcatgacgt
     1621 cggggtactc accgattggg gcgatgatcg cgtccgaccg gctgttcgag cccttctccg
     1681 atggcaagac gacattcacc cacggctaca cgttcggcgg gcatccggta tcggccgcgg
     1741 tggcccttgc caacctcgac gtcttcgagc gcgagggcct caacgaccac gtgaagcaga
     1801 ccgcgccggc cttccgcggg acgctcgaga agctgctcga cctgccgatc gtcggcgacg
     1861 tgcgcggcga gggcttcttc tacggaatcg aactggtcaa agacaaggcc accaagcagg
     1921 tcctcgacga cgagctcagc gaatggctgc tgcgcagctt tttgtccacc gagctcttcg
     1981 aggccggcct gtactgccgc gccgacgacc gcggcgactc ggtgatccag ctggcgcccc
     2041 cgttgatcag tggccaggcc gagttcgatg agatcgagca gatcctgcgc ggtgtgctga
     2101 ccgaggcgtg ggcacgcctg caaaaccatg gtgaaccctg gcactaaatc ggggatagac
     2161 ccggtccgcc ggtacgctcc accgatcggg gaactgccca cttataccga cctgtaaccg
     2221 cccggcagcg ggtcgatgcc ccgcagcgtg accttcgtcc cgtaatctgc cgtgtcgatg
     2281 ggtgggatca ggtgtgccgc gtgccgcgtg ctcggcgcgg tctctgcggc ggtgctggtg
     2341 gggtttccga cgctgggcgc cgtgcccgtc gcgtccgcgg agccggtggc atctccagac
     2401 accgcatcgt gcccgtacca gctggccacg ccgcccgccg tcgacgaatc cgaagtcccg
     2461 gtcgcgggtg atccgcctct gccgctgccg gtgccggcca agcccgccgg cggcgacggc
     2521 ctcagcggct gcggtgtggt cgtcgcgccg aacacgcccc cggtgcccga cgacatctcc
     2581 gccgaagcgt ggctggtcgc cgatctggac accggcgacg tgatcgccgc gaaggatccg
     2641 cacggccggc accggccggc cagcatcatc aaggtgctgg tggcgatgca ggcgatcaac
     2701 gaggtgggcg tcggcaaggt ggtcaccggc accgcagacg acggcaacgc cgaaggcacc
     2761 cgcgtcggcg tcaacgaggg cggcacctac accgtctatc agctgctgca cgggttgctg
     2821 atgcactccg gcaacgacgc ggcgcacgcg ctggcgatgc agctgggcgg gatggacgtg
     2881 gcgctggaca agatcaacac gctggcccgc aagctcggag ccaccgacac ccgggcggcg
     2941 acaccgtccg gtctggacgg cccgggcatg agcacgtcgg cgtacgacat gggcctgttc
     3001 taccggtacg cctggcagaa cccgatcttc gcgcagatcg tcgcgacgag gacgttcgaa
     3061 ttcccggata gtcacggcgg cgcgccatac ccggtggaga acgacaacca gctgctgtac
     3121 aactaccccg gcgcgctcgg tggcaagacc ggctacaccg acgacgccgg ccagaccttc
     3181 gtcggcgcgg ccagccgcga cggccggcgc ctagtggtgg tgttgctgca cggcacccgg
     3241 cagccaatcc cgccgtggca gcaggccgcg catttgctgg actacgggtt cgcgacgccg
     3301 ctcggcacca aggtcggcaa gctggtcgac ccggacccgt cgctggtgcc gaaaccctcc
     3361 gattcccccg tttccgccgc cggacccggc ctgccgggcg cgatgaacga gctgccggtg
     3421 cgggtggggg tggcgatcgt gggcgcgatg atcgtgttcg gcctgatcct cgccgcgcga
     3481 tcggtcaacc ggcgaacgca gaattagtct ttacccccgt cgccgagtgt gcgctggctg
     3541 cgaaaaacct tggcttggga aagtcccatt caacgaatag tttagattcg acgtgaaggt
     3601 cgggctgtat ttcgatttac gaaatccgac gggttggcgg caggaccccg cgcgtctgca
     3661 cgcgttcacg ctggaagcga tcgaagaggc cgagcatctg ggcatcgact cgatctg
//
LOCUS       DASWBF010000072        20369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1122011,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000072 DASWBF010000000
VERSION     DASWBF010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20369
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122011"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VF874_02010"
     CDS             complement(<1..585)
                     /locus_tag="VF874_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEX9174900.1"
                     /translation="MSRLHPIVSVTGSSGAGTTSVMRTFERIFRREGVRATFVEGDSF
                     HRYDRVQMKAAITDAKARGDHSFSHFGPETNLFKELEELFRVYGETGTGKVRRYLHDD
                     VEAEPHGQEAGTFTPWEPLPEESDMLFYEGLHGAIVTDEVDVARYADLRIGVVPVINL
                     EWIQKLHRDKVDRGYTHEDVTDTILRRMPDYVKYV"
     gene            complement(726..1595)
                     /locus_tag="VF874_02015"
     CDS             complement(726..1595)
                     /locus_tag="VF874_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174901.1"
                     /translation="MLAVTAGCAAPQSQPHPYGAATAKIGESLALLGWNISISNLRWE
                     SGRVLVDVDASPSEQGKPHAKPEDIRLGLYGALAHPIEADGLGSCNDVASLAISPLSA
                     PNPDRLTGTVCLGPLENQSEMRGVYVYSPRDRIAGTTAAYPVAFPVGVLPTNRNDTGV
                     TVKSTSVEAWRADGAMLSQASLGDPAIFTGNGYMLLGLEISAIKEQYSDESTERGGPL
                     MVVVAPTLPAGQGPSHACATYGSSVLVLPDAKLDAVQVDASLCSQGEINQAVLYATLS
                     VLGTHAAVWISRD"
     gene            complement(1633..2418)
                     /locus_tag="VF874_02020"
     CDS             complement(1633..2418)
                     /locus_tag="VF874_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016691494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9174902.1"
                     /translation="MTAPESIFVTGAARGIGLAVVRRFAAAGYTVGAVDVDETELDNL
                     KTEAAQHGRSVWTSAMDVTDFDAWQRTLSEFVSTTGGRLDILVNNAGVLAAGNFVDIP
                     PQRHKQIVDVNVTGVIFGCHAAFEYLRDTAGARVLNLCSASAIYGQAELASYSATKFA
                     VRGLTEGLDLEWRPHDITVSALWPLFVDTAMLTGVHTGATESLGIHLTPDDIADAAWR
                     ALHRKRGMPKVHYPVGTQTKLAYYFLQVSPTWMSRLVNKRLTH"
     gene            complement(2405..3178)
                     /locus_tag="VF874_02025"
     CDS             complement(2405..3178)
                     /locus_tag="VF874_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9174903.1"
                     /translation="MPRPVALITGPTSGIGAGFARRYAADGYDLALVARDTERLRGLA
                     DTLYQNHGTNSEVLTADLADADGRAAAAERLGNGVRVLVNNAGFGTSGEFWTTDPAVL
                     QAQLDVNVTAVMHLTRAALPPMLDAGIGTVINVASVAGLLPGRGSTYSASKAWVVSFS
                     EGLANGLSGTGVGVHALCPGFVHTEFHERAGMEKPMPEFWWLHVEDVVDDCLADVAKG
                     KVVIIPALQYKTLTTASRLVPRNLVRLMTKTVGRGRDRT"
     gene            complement(3185..3994)
                     /gene="ttfA"
                     /locus_tag="VF874_02030"
     CDS             complement(3185..3994)
                     /gene="ttfA"
                     /locus_tag="VF874_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose monomycolate transport factor TtfA"
                     /protein_id="HEX9174904.1"
                     /translation="MVPLWFTLSALCFVTAGVLLYVDLDRRHNHGRRRKSWARSHGFD
                     YEPESGEILERWKRGVMSTVGDVSARNVVLGQIRGEAVYIFDLEDVATVIALHRKIGT
                     NVVMDLRLKGLKEPRENDVWLLGAIGPRMVYSTNLDAARRACDRRMVTFAHTAPDCAE
                     IMWNEQNWTLVSMPITSTRAEWDEGLRTVRQFNDLLRVLPPLPGKAIGQPAARRNGAP
                     GRPVAPPAELPPGRPPTDYRGEPPRQQPQSEPIHRPQQTRAGRPGPPSYQP"
     gene            4071..4883
                     /locus_tag="VF874_02035"
     CDS             4071..4883
                     /locus_tag="VF874_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9174905.1"
                     /translation="MAYRDYQYLRIAVDDRICRATIDNPPMNLADLNLFGEVGRFALE
                     VVADDDVHVVLVDSADPDFFVAHADVTMLQALPTDDTSLHDELSAFHAVVELFRTMPK
                     ATIAVIEGIARGGGSEFALSFDMRFAALGKARLAQPEVAVGIIPGGGATQRLPRLIGR
                     ARTLEIVLGCMDIDAATAEAWGYVNRALPPDELRPFVDTLATRIASYPLDVIARAKRA
                     VDAAVGDPIPGLNVEDQLFREAMAGPAAAERMAAFMAGGGQTRDYELGETPF"
     gene            4938..5117
                     /locus_tag="VF874_02040"
     CDS             4938..5117
                     /locus_tag="VF874_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEX9174906.1"
                     /translation="MSRSEFFTRAAERYLDELDARSLTGQIDDALKHLSAPDEATVAA
                     IAAGHRVLDAVADEW"
     gene            5111..5203
                     /locus_tag="VF874_02045"
                     /pseudo
     CDS             5111..5203
                     /locus_tag="VF874_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5266..7812)
                     /gene="clpB"
                     /locus_tag="VF874_02050"
     CDS             complement(5266..7812)
                     /gene="clpB"
                     /locus_tag="VF874_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX9174907.1"
                     /translation="MDSFNPTTKTQAALTAALQAASAAGNPEIRPAHLLMALLTQNDG
                     IAAPLLEAVGVDPATIRMETQRLLDQLPSASGASSQPQLSRESLAAISAAQQLATEMD
                     DEYVSTEHLMVGLATGDSDTAKLLTGHGASPQALRDAFVKVRGSARVTSPDPEGTYQA
                     LEKYSTDLTARAKEGKLDPVIGRDNEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVAGDVPESLRDKTIVALDLGSMVAGSKYRGEFEERLKAVLDDIKNSAGQIITF
                     IDELHTIVGAGATGEGAMDAGNMIKPMLARGELRLVGATTLDEYRKHIEKDAALERRF
                     QQVFVGEPSVEDTVGILRGLKERYEVHHGVRITDSALVAAATLSDRYITSRFLPDKAI
                     DLVDEAASRLRMEIDSRPVEIDEVERLVRRLEIEEMALEKEEDEASKDRLEKLRAELA
                     DQKEKLAELTTRWQNEKNVIDIVRDLKEQLDTLRQESERAERDGDLAKAAELRYGRIP
                     EVEKQLDAALPQAQAREDVMLKEEVGPDDIADVVSAWTGIPAGRMLEGEAAKLLRMED
                     ELGKRVIGQKKAVQAVSDAVRRSRAGVADPNRPTGSFMFLGPTGVGKTELAKALAEFL
                     FDDERAMVRIDMSEYGEKHSVARLVGAPPGYIGYDQGGQLTEAVRRRPYTVVLFDEVE
                     KAHPDVFDVLLAVLDEGRLTDGQGRTVDFRNTILILTSNLGSGGSEEQVMAAVRATFK
                     PEFINRLDDVLIFEGLNPEELVQIVDIQLAQLQKRLAQRRLQLEVSLPAKKWLAQRGF
                     DPVYGARPLRRLVQQAIGDQLAKMLLSGDVHDGDTVPVNVSPDGDSLVLG"
     gene            7994..8662
                     /locus_tag="VF874_02055"
     CDS             7994..8662
                     /locus_tag="VF874_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX9174908.1"
                     /translation="MAANWGTVLTELIPLALVITLSPLSIIPAVLVLHTPHPRPAALL
                     YLVGWVVALFTLTAVFVGVSGLVGSLESPPKWASWLRIVVGAALIVFGILRWLTRHRS
                     PHMPAWMRQLTSVTPAKAGVYGAVLAVVNPKVLFICAAAGLAIGTEGIGAPGAWLAVA
                     YFVAIAAFSVALPILAYAVSGDRLDGPLSKLKDWMERHNAALVAAILVVIGLLVLYKG
                     VHGL"
     gene            complement(8657..9292)
                     /locus_tag="VF874_02060"
     CDS             complement(8657..9292)
                     /locus_tag="VF874_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9174909.1"
                     /translation="MSRPATINKRRGRRQGEPVSRDAVLRAAKRQFARHGYDKTTLRA
                     IAHEAHIDASMVLYLFGSKAELFRESMRLILDPDVLAAAIAHGDGDIGNRMARAYLRI
                     WEQPDTAASMVSMLQSATSNADAHDAFRGFMQNYVLTAVSGALGGGEDARLRAMLAAT
                     NLVGTAILRYVMRVPPLATLDVDDVVALIGPAVHRYLTAPADELGLDRLSL"
     gene            9306..10091
                     /locus_tag="VF874_02065"
                     /pseudo
     CDS             9306..10091
                     /locus_tag="VF874_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            10103..11269
                     /locus_tag="VF874_02070"
     CDS             10103..11269
                     /locus_tag="VF874_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9174910.1"
                     /translation="MGLEDRDALRLLRSALDPPDADNELVCRFYTRWFAIDPSIRDLF
                     PPDMDGQRAAFGEALRWVMGELVARRSDEPVAFLAQLGRDHRKYGVTQQHYQTMQEAL
                     YSSFRSHLIDCWDDAVDEAARDALALIIGVMSGAADAESGPAWWDGTVVEHQRVSRDV
                     AVVRLQLDRPMHYYAGQYVNVQVPQCPRRWRYLSPAIPADPGGTIEFHVRTVLGGMVS
                     SAVISETRLGHRWRLSAPHGAMEVDREGGDVLMVAGSTGLAPLRALIMDMTRFAHNPR
                     VHLFFGARYPCELYDLRTLWSIAAHNPWLSVSPVSEYNHNPPWAGDYPDVQPPRGLHV
                     RQTGRLPEVVTKYGAWGDRQILISGNPQMIANTKAALVAKGAPLERIQHDPLVP"
     gene            11319..12218
                     /locus_tag="VF874_02075"
     CDS             11319..12218
                     /locus_tag="VF874_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEX9174911.1"
                     /translation="MADLETITLKDPSSSLTATYVPRAGMVGASLSDDGVELLGQRRG
                     LHAYMTTGKTMGIPILYPWANRLSANTYQVGTGAVTLTPGIGGVRTDQHGVVIHGILA
                     ASEGWEVKDQTENRLTAELDYGGTPRLLVSFPFPHVLTVDVTLADRVLTVRTTVTATT
                     RADVPLCFGFHPYLRIADVPRAEWEMELPAMRYCPVNAWMIPTGETEDRPASTETLGD
                     NYFDNSYDEVKPGSVFAVSGGDRRIEVHFDEGYTAAQVFSPSDDEVICFEPMCAPTDS
                     LRRGTYRVATEGEPATATFSIHV"
     gene            complement(12345..12731)
                     /locus_tag="VF874_02080"
     CDS             complement(12345..12731)
                     /locus_tag="VF874_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9174912.1"
                     /translation="MSADETRTFLISVAAQLAGMHAQTLRTYDRLGLVTPRRTMGGGR
                     RYSQRDVDLLREVQRLSQDEGVNLAGIKRIIELTNQVEALQSRLRELTEEVQQLRTNQ
                     RRDVAVVPKSTALVVWEPRQSRRSRR"
     gene            complement(12728..13906)
                     /gene="dnaJ"
                     /locus_tag="VF874_02085"
     CDS             complement(12728..13906)
                     /gene="dnaJ"
                     /locus_tag="VF874_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9174913.1"
                     /translation="MAQREWVEKDFYKELGVSSDASEDEIKRVARKLLAENHPDRNPG
                     NAAAEERYKAVAEAKEVLSDPAKRKEYDETRRLFAEGGFGRRRFSAGNFGGFGTGGNG
                     TEFNLNDLFDSAGQTGGANIGDLFGGLFGRGAQPRPSRPRRGNDLETETELDFVEATK
                     GVAMPLRLTSPAPCTNCHGSGARPGTSPKVCSSCNGSGVINRNQGAFGFSEPCTDCRG
                     TGSIIEHPCPDCKGTGVATRTRTINVRIPPGVQDGQRIRLPGQGEAGLRGAPSGDLYV
                     TVHVRPDKVFGRDGDDLTVTVPVSFVELALGTTLSVPTLDGKVGVRVPKGTADGRILR
                     VRGRGVPKRDGSNGDLLVTVKVAVPPNLEGAALEALEKYAAAEKASGFDPRSGWAGRL
                     "
     gene            complement(13930..14556)
                     /gene="grpE"
                     /locus_tag="VF874_02090"
     CDS             complement(13930..14556)
                     /gene="grpE"
                     /locus_tag="VF874_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9174914.1"
                     /translation="MTPDEGHEPVTITDKRRIDPETGEVREGAAGPAPSGPAPGVPDT
                     EEAAELKATLQRVKAEYDNYRKRSVRQEQLAAERAKAAVVAQLLGVLDDLDRARNHGD
                     LESGPLKSVADKLTGAMEGLGLTAFGDEGDDFDPSLHEAVQHEGDGTKPVVGTVMRRG
                     YRLGDQVLRHALVGVVDTVDDEPLARRATGEEADADAEDTAESEESAS"
     gene            complement(14553..16409)
                     /gene="dnaK"
                     /locus_tag="VF874_02095"
     CDS             complement(14553..16409)
                     /gene="dnaK"
                     /locus_tag="VF874_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9174915.1"
                     /translation="MARAVGIDLGTTNSVVAVLEGGDPVVVANSEGSRTTPSVVAFAR
                     NGEVLVGQPAKNQAVTNVDRTIRSVKRHMGTDWSVDIDGKNYTAPEISARILMKLKRD
                     AESYLGEDITDAVITTPAYFNDAQRQATKDAGQIAGLNVLRIINEPTAAALAYGLDKA
                     DKEQTILVFDLGGGTFDVSLLEMGEGVVEVRATSGDNHLGGDDWDDRIVEWLVDKFRG
                     TSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINLPYITVDADKNPLFLDEQLTRA
                     EFQRITQDLLDRTRKPFQSVIADASISVADIDHVVLVGGSTRMPAVSDLVKEMTGGKE
                     PNKGVNPDEVVAVGAALQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTKLIERNTTI
                     PTKRSETFTTADDNQPSVQIQVYQGEREMASANKLLGSFELTGIAPAPRGVPQIEVTF
                     DIDANGIVHVTAKDKGTGKENKIQIQEGSGLSKEEIDRMIKDAEAHADEDRNRREEAD
                     VRNQAETLVYQTEKFVEEQREAEGGSKVPEDTLNKVDAAIADAKTALGGTDINAIKSA
                     MEKLGQESQALGQAIYEATQAAQAAGGDGAESAPGGDDVVDAEVVDENQEDR"
     gene            complement(16591..17388)
                     /locus_tag="VF874_02100"
     CDS             complement(16591..17388)
                     /locus_tag="VF874_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9174916.1"
                     /translation="MQHPTPRFLAQDIFASLRRWTGDSIPDQMPGIEATVDAMDAAGV
                     GFGLLSAWSAPHQEPLISNDEVAGWVAAHPDRLAGLACVDLDKPMSAVRELRRCVTEL
                     GFKGLRVVPWLWNAPPTDRRYYPLYAECVQLGVPFCTQVGHTGPLRPSETGRPIPYID
                     QVALDFPELTIVCGHVGYPWTEEMIAVARKHDNVVIDTSAYTTKRLPPELVTYLKTGS
                     GQHKVLFGSNYPMIAPAHALDGLDDLGLSDEARSLYLAGNARRVFGI"
     gene            complement(17458..17712)
                     /locus_tag="VF874_02105"
     CDS             complement(17458..17712)
                     /locus_tag="VF874_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174917.1"
                     /translation="MSTNWIRRTSRNTATVYQVADRLHRGRAARVSSNEIAATVSAWL
                     AELEVETPMVDELAQAVRTGNWTAAHALEDLLSVDVTVAA"
     gene            17875..18429
                     /locus_tag="VF874_02110"
     CDS             17875..18429
                     /locus_tag="VF874_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174918.1"
                     /translation="MREGEAVRKSAVALMAAAIALTGCTSWTNGKPVAVPSNPTEPTF
                     PTTRPSRTPPSTPSSTTPVPAPTPPPGGETLDPNEQGYVYIETKSGKTRCQISQTAVG
                     CEAPFTNSPIQGGIPANGVDVTAGGDFRWLVGNLGDITPVTLDYRTYQAQGWTIAADE
                     DGTRFTNNRSGHGMFVSIEKVESF"
     gene            complement(18466..18819)
                     /locus_tag="VF874_02115"
     CDS             complement(18466..18819)
                     /locus_tag="VF874_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9174919.1"
                     /translation="MMGKGRDDLVFADSRGGVLRHSNYRNRTFASAVQKCQADDDAFP
                     SITPHDLRHTAASLAISAGANVKAVQRMLGHAKASMTLDVYADLFDEDLDGVADRLDT
                     AIRSTADHLRSRTVR"
     gene            18846..18971
                     /locus_tag="VF874_02120"
     CDS             18846..18971
                     /locus_tag="VF874_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174920.1"
                     /translation="MRVRFDITLEPSAPLFEFLIKRGETIVLDAATEGLGTRVLG"
     gene            19476..19682
                     /locus_tag="VF874_02125"
     CDS             19476..19682
                     /locus_tag="VF874_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX9174921.1"
                     /translation="MSGTVARVTEISARSDTSFEDAAKGGLARANETLRGVKSAWIKE
                     QSVEVENGKIVAYRVNMLVTFVLD"
     gene            complement(20016..>20369)
                     /locus_tag="VF874_02130"
     CDS             complement(20016..>20369)
                     /locus_tag="VF874_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX9174922.1"
                     /translation="LHRGLVLIKWWLLAVPHYLVLVAFFYGGVGAFMGNDDDDWGDGT
                     ILVPLIRVLLLIAVVALLFTARYPKGLYDFVIGINRWAIRVFAYTSLMRDEYPPFRLD
                     MGPREPETPSASQSA"
BASE COUNT         3116 a         6921 c         6691 g         3641 t
ORIGIN      
        1 cacgtacttc acgtaatccg gcattcgccg caggatcgtg tcggtcacgt cttcgtgcgt
       61 gtatccgcga tccaccttgt cgcggtgcag tttttgaatc cactccaggt tgatcaccgg
      121 aacgacaccg atccgtaggt cggcatatcg agcgacatcc acctcgtcgg tcacgatcgc
      181 accgtgcagt ccttcgtaga acagcatgtc ggactcttcg ggaagcggct cccacggcgt
      241 gaacgtcccc gcctcctggc cgtgcggttc tgcttcgacg tcatcgtgca gatatctgcg
      301 gaccttgccc gtccccgttt caccgtagac ccggaacagc tcttcaagtt ccttgaacag
      361 gttggtttcc ggcccgaagt gactgaacga atggtccccg cgggccttcg cgtcggtgat
      421 ggcggccttc atctggactc tgtcgtagcg atgaaagctg tcgccctcga cgaatgtggc
      481 tcgcacaccc tcgcgacgaa aaatccgctc gaaggtgcgc atgaccgagg tcgtgccggc
      541 cccagacgaa ccggtgaccg acacgatcgg gtgtaagcgg gacatgacgt tgtggtggcg
      601 gtcagccgtg gctcgtggtc aggcgatcgc caaacatggc acccacaatg acaggagcga
      661 ttgcgcccgc catcccccgc ccgggggact gtgtgggggc tcagctgctc acccggacac
      721 tacgctcagt cgcggctgat ccataccgcg gcgtgcgtgc ccagcaccga gagcgtcgcg
      781 tacagaaccg cttggttgat ctcaccttgg ctgcacagcg acgcgtccac ctgcaccgcg
      841 tcgagcttcg cgtcgggcag caccagtacc gacgacccgt acgtcgcgca ggcgtggctg
      901 ggcccctgcc ccgccggcag cgttggcgcc accaccacca tcagcggccc tccgcgctcg
      961 gtggattcgt cgctgtactg ttccttgatc gccgaaatct caaggcccag cagcatataa
     1021 ccgttgcccg tgaagatcgc gggatctccg agggacgcct ggctgagcat ggctccatcg
     1081 gctcgccacg cttccacgct ggtcgacttg acggtgacgc cggtgtcgtt gcggttcgtc
     1141 ggcaagactc cgaccgggaa cgcaaccggg tacgcggcgg tggtgccggc gatccggtcg
     1201 cgtggcgagt acacgtacac gccgcgcatt tcgctttggt tctccaacgg gcccaggcac
     1261 acggtgccgg tgagccggtc agggttgggc gcggacaacg gcgaaatcgc gagcgaagca
     1321 acgtcgttgc agctgcccag gccgtcggct tcgatcgggt gggcgagtgc gccgtacaga
     1381 cctaagcgga tgtcctccgg tttggcgtgc ggcttgccct gttcggacgg ggaagcatcg
     1441 acgtcgacga gcacgcggcc cgactcccag cgcaggttgg atatcgagat gttccaccca
     1501 agcagggcca acgattcacc gatcttcgcc gtggctgcac cgtacgggtg aggctggctc
     1561 tgcggcgcag cgcagcccgc agtgacagcg agcacggccg tcgacagcgc ggcgaagaga
     1621 gcacgcaagg tcttagtggg tcagccgctt gttgaccagc cgcgacatcc acgtcgggct
     1681 gacttgcaag aagtagtacg ccaacttggt ctgcgtgccc accgggtaat gcactttcgg
     1741 cattcctcgc ttgcggtgca gcgcacgcca cgcggcatcg gcgatgtcat cgggcgtgag
     1801 gtggattccc agcgactcgg tggcgccggt atgcactccg gtgagcatcg ccgtgtcgac
     1861 gaacaacggc cacagcgcgg acacggtgat gtcgtgggga cgccattcca gatccagccc
     1921 ctcggtgagg ccgcggaccg cgaatttcgt tgcgctgtaa gatgcgagct cggcctgccc
     1981 gtagatcgcc gacgccgagc agaggttcag gacgcgagcg ccggcggtgt cacgaaggta
     2041 ctcgaacgcg gcgtggcagc cgaatatcac tcccgtgacg ttgacgtcga cgatctgctt
     2101 gtgccgttgc ggcgggatgt cgacgaagtt gccggcggcc agcacacccg cgttgttgac
     2161 caggatgtcc agccgtccac ccgtggtgct gacgaactca ctgagcgtgc gttgccacgc
     2221 gtcgaagtcg gtaacgtcca ttgctgaggt ccataccgac cgaccatgtt gtgctgcttc
     2281 tgttttcagg ttgtcaagtt ctgtctcgtc gacgtcgacc gcgccaacgg tgtaacccgc
     2341 ggcggcgaac cggcgcacga ccgccagccc gatgccgcgc gcggcgcccg tgacgaagat
     2401 cgactcaggt gcggtcacgg ccacgtccca ccgtcttcgt catgagcctc accaggtttc
     2461 gcggcaccaa ccgtgaagcg gtggtcaacg tcttgtactg caacgcggga atgatcacca
     2521 ccttgccttt cgcgacatcg gccagacagt cgtcgacgac gtcctcgacg tggagccacc
     2581 agaactccgg catcggtttt tccatgccgg cgcgctcgtg gaattcggtg tgcacgaagc
     2641 ctggacacag cgcgtgcaca cccacaccgg tgccggacag cccgttggct aggccttcgg
     2701 agaacgacac cacccacgcc ttggacgccg agtacgtcga gccgcggccg ggtagcagcc
     2761 cggcgacgct ggcgacgttg atcacggtgc cgattccggc gtcgagcatc ggcggcagcg
     2821 ccgcgcgggt gaggtgcatg accgcggtga cgttgacgtc gagctgggcc tgcagcaccg
     2881 cgggatcggt ggtccagaac tcgccggacg tgccgaagcc ggcgttgttc accagcacac
     2941 gcacaccgtt gcccagccgc tcggcggcag ctgcgcgccc gtcggcgtcg gcgaggtcgg
     3001 cggttaggac ttcgctgttg gtgccgtggt tctggtacag ggtgtcggcc agcccgcgca
     3061 gccgttcggt gtcgcgcgcg accagcgcca ggtcgtaccc gtcggcggcg tagcgccggg
     3121 cgaagcccgc gccaatgcca gacgtgggac cggtgatcag ggcgacggga cgcggcatcc
     3181 gcggctacgg ctggtagctg ggcggaccgg gccgcccggc gcgcgtctgc tgcgggcggt
     3241 ggatcggctc agactgtggc tgttgacgcg ggggctcgcc gcggtaatcc gtcggagggc
     3301 gtcccggcgg aagctcggct ggcggcgcaa ccggtcgacc gggcgcgccg ttgcggcggg
     3361 cggcgggctg cccgatggcc ttgcccggta acggcggcag cacccgcagc aggtcgttga
     3421 actgccggac agtgcgcagg ccctcgtccc actcggcgcg cgtgctggtg atcggcatgc
     3481 tcaccagcgt ccagttctgc tcgttccaca tgatttccgc gcagtcggga gcggtgtgcg
     3541 cgaacgtcac catccggcga tcgcacgcgc gccgcgcggc gtcgaggttg gtggagtaca
     3601 ccatccgcgg gccgatagcg ccgagcagcc acacgtcgtt ctcccgcggc tccttgagtc
     3661 ccttgagccg caggtccatc acgacgttgg tgccgatctt gcggtgcaac gcgatcacgg
     3721 tcgcgacgtc ttcgaggtcg aagatgtaga ccgcctcgcc gcggatctgg cccagcacca
     3781 cgttgcgcgc gctcacgtcg ccgaccgtgg acatcacgcc gcgcttccag cgttcgagga
     3841 tctcgcccga ctcgggctcg tagtcgaacc cgtgtgagcg agcccaggac ttgcggcgcc
     3901 ggccgtggtt gtgccgtcga tcgaggtcga cgtacagcag cacccccgcg gtcacgaaac
     3961 agagcgcgga cagcgtgaac caaagcggaa ccattaggcc tagcctatct gctgccggcg
     4021 gcgattcccg aactcgcgcg cgtctcttgc gcgagccgta acgtcggctc atggcatatc
     4081 gcgactacca gtaccttcgc atcgcggtcg acgaccggat ctgccgggcg acgatcgaca
     4141 atccgccgat gaacctcgcg gatctcaacc tcttcggcga agtcggccgc ttcgcactgg
     4201 aggtcgtcgc cgacgacgac gtgcacgtgg tgctcgtgga ttcggccgac ccggacttct
     4261 tcgtcgccca cgccgacgtg acaatgctgc aggcactgcc gaccgatgac acctcgctgc
     4321 acgacgagct ctcagcgttc cacgccgtcg tcgagctgtt ccgcaccatg ccgaaggcga
     4381 cgatcgcggt gatcgagggg attgcccgcg gtggcggcag cgagttcgcg ttgtcgttcg
     4441 acatgcggtt cgccgcgctg ggcaaggcgc gcctagcgca acccgaggtc gcggtcggaa
     4501 tcatccccgg cggcggcgcg acgcaacgac tgcctcggct catcggccga gcccgcacgc
     4561 tggagatcgt gctcggctgc atggacatcg acgcagcgac cgccgaggcg tggggatacg
     4621 tcaaccgcgc gctgccgccc gacgagctac gcccgttcgt cgacaccctg gcgacacgta
     4681 tcgcgtcgta tccgctcgat gtgatcgcgc gggccaaacg cgcggtggac gccgcggtcg
     4741 gcgacccgat ccccggcttg aacgtcgaag atcaactgtt ccgcgaagct atggcagggc
     4801 cggccgccgc tgaacgcatg gcggcattca tggccggcgg cggccagacc cgcgactacg
     4861 agctcggcga aacgccgttc tagagacgct ccgaccagac gttcgagcgc gcctcgcgcc
     4921 gtgccggcga tcttggcatg agccggtccg aattcttcac tcgtgctgcc gagcgctacc
     4981 tcgacgaact ggacgcccgg tctctaaccg gccagatcga cgacgccctc aaacacctga
     5041 gcgcaccgga tgaggccact gtcgccgcca tagcagctgg ccaccgcgta ctcgatgccg
     5101 tggccgacga gtggtgatcc accgctcaga catttactgg gccgatctcg gacctgtatc
     5161 cggcagccga ccggccaagc gccgtccggt ggactgcgtc gagttctcgc gcgatgaccc
     5221 gcgattcagc aactcagccg aggctgatgg tcttcgcgcg agcgctcatc cgagcaccag
     5281 cgaatcgccg tccgggctga cgttgaccgg aaccgtgtcg ccgtcgtgga cgtcgccgga
     5341 cagcagcatc ttggccagct ggtcgccaat ggcctgctgc accaacctgc gcagcgggcg
     5401 cgcgccgtac acggggtcga acccgcgctg ggccagccac ttcttcgcgg gcagcgacac
     5461 ctccagttgc agccgccgct gagccagccg cttctgcagc tgtgccagct ggatgtcgac
     5521 gatctgcacc agctcttcgg ggttgaggcc ctcgaagatc agcacgtcgt ccagccggtt
     5581 gatgaactcg ggcttgaacg tcgcgcgcac ggccgccatc acctgctcct cgctgccgcc
     5641 cgacccgagg ttggacgtca ggatcaggat cgtgttgcgg aagtcgaccg tgcggccctg
     5701 cccgtcggtt agccgaccct cgtcgaggac agccagcagc acgtcgaaca cgtccgggtg
     5761 cgccttctcg acctcgtcga acagcacgac cgtgtacgga cgtcgccgca ccgcctcggt
     5821 cagctggccg ccctggtcat acccgatgta gccgggaggg gccccgacca gccgggccac
     5881 cgagtgcttc tcgccgtact cgctcatgtc gatgcgcacc atcgcgcgtt cgtcgtcgaa
     5941 caagaactcc gcaagcgcct tggccagctc ggtcttaccg acgccggtcg gaccgaggaa
     6001 catgaacgag cccgtcggcc ggttcgggtc ggcgacaccg gcccgactgc gccgcacggc
     6061 atccgagacc gcctgcacgg ccttcttctg gccgatgacg cgcttgccca gctcgtcttc
     6121 catccgcagc agcttggcgg cttcgccttc gagcatccgg ccggccggga tcccggtcca
     6181 cgccgagacc acgtcggcga tgtcgtcggg tccgacctct tccttgagca tcacgtcctc
     6241 gcgcgcctgc gcctgcggca gcgcggcgtc gagctgcttc tcgacctcgg ggatgcgccc
     6301 gtagcgcagc tcggccgcct tggccaggtc gccgtcgcgc tcggcccgct ccgactcctg
     6361 acgcagcgtg tccagctgct ccttgagatc acggacgatg tcgatcacgt tcttctcgtt
     6421 ctgccagcgg gtggtcagct cggcgagctt ctccttctga tcggcgagtt cggcgcgcag
     6481 cttctccagt cggtccttgg aggcctcgtc ctcttccttc tccagcgcca tctcttcgat
     6541 ctccagccgg cgcaccagcc gctcgacctc gtcgatctcg acgggccgcg agtcgatctc
     6601 catcctcagg cgggaggcgg cctcgtcgac caggtcgatg gccttgtccg gcaggaagcg
     6661 cgaggtgatg tagcggtcgg acagcgtcgc ggcggccacc agcgcggagt cggtgatccg
     6721 cacgccgtgg tgcacctcgt agcgctcctt gaggccgcgc aggatgccga ccgtgtcctc
     6781 gaccgacggc tcgccgacga acacctgctg gaagcggcgc tccagcgcgg cgtccttctc
     6841 gatgtgcttg cggtactcgt cgagcgtggt cgcgccgacc agccgcagct cgccgcgggc
     6901 cagcatcggc ttgatcatgt tgccggcgtc catcgcaccc tcgccggtgg cgccggcgcc
     6961 gacgatcgtg tgcagctcgt cgatgaacgt gatgatctgc ccggcggagt tcttgatgtc
     7021 gtcgagcacg gccttcagcc gctcctcgaa ctcgccgcgg tacttcgacc cggccaccat
     7081 cgaccccagg tccagcgcga caattgtctt gtcccgcaag ctttccggca catcgccggc
     7141 cacgatccgc tgggccaggc cctcgacgat cgcggtcttg ccgacgccgg gctcgccgat
     7201 cagcaccggg ttgttcttgg tgcgacggga gagcacctgc acgacgcgtc gaatctcgtt
     7261 gtcgcgcccg atgacggggt cgagcttgcc ctctttggcg cgggcggtca ggtcggtgga
     7321 gtacttctcc agcgcctggt aggtgccctc cgggtcgggg ctggtgaccc gcgcgctgcc
     7381 gcggactttg acgaaggcgt cccgcagagc ttgaggcgaa gcgccatggc ctgtgaggag
     7441 tttggctgtg tcggaatctc cggtggccag cccgaccatc aagtgttcgg tggagacgta
     7501 ctcgtcgtcc atctcggtgg ccagctgctg cgcggcgctg atggcagcca gcgattcgcg
     7561 ggacagctgc ggctgactgc tggcgccgct ggcgctgggc agctggtcga gcagccgctg
     7621 cgtctccatg cggatggtcg ccgggtcgac gccgacggcc tccagcagcg gcgcagcgat
     7681 gccgtcgttc tgcgtgagca gggccatcag cagatgggcc gggcggatct ccgggttacc
     7741 tgcggcgctg gccgcctgca gtgccgcggt caacgccgcc tgagtcttcg tggtcgggtt
     7801 gaacgagtcc acgacacctc cgatcgtcta aatggggcca gaaaaatgct tgtcgggaag
     7861 tccaacgccg tcaaggttga gtctgttccg ctcaactcta cctttttcgc gacagtgcgg
     7921 cttcgtacgg gacacgccgc ggggtgcgca taaatccgca cagtcgacgc aatccgcgac
     7981 atgatcggct gttatggccg cgaactgggg caccgtgctg acggaactca tcccgttggc
     8041 gttggtgatc acgctgtcac cgctgtcgat cattccggcc gtcctggtgc tgcatacccc
     8101 gcacccccgg ccggccgcgt tgctgtacct ggtcgggtgg gtggtcgcgc tcttcacgct
     8161 cacggccgtt ttcgtcgggg tgtccgggct cgtcggaagc ctcgagagcc cacctaagtg
     8221 ggcgtcgtgg ctgcgcatcg tcgtcggcgc cgcgctgatc gtattcggca tcctccgctg
     8281 gctgacccgc caccggtccc cacacatgcc ggcctggatg cgtcagctca ccagcgtcac
     8341 acccgcgaag gcgggcgtct acggtgcggt attggcggtg gtgaacccga aggtgctgtt
     8401 catctgcgcc gcagcgggat tggcgatagg caccgagggg atcggcgcac ccggagcgtg
     8461 gctggcggtc gcgtacttcg tcgcgatcgc cgcattctcg gtcgccttgc ccatcctggc
     8521 gtacgcggtg tccggcgacc gcctggacgg gccgctgtcc aagttgaagg actggatgga
     8581 gcggcacaac gccgcgctgg tggcggcgat cctcgtcgtg atcggtctgc tggtgctcta
     8641 caagggcgtc cacggcctat aacgacagcc gatccaagcc gagctcgtcg gcgggggcgg
     8701 tcaggtatcg gtgcaccgcg gggcctatca gcgccaccac gtcgtcgaca tccaaggtcg
     8761 ccagcggcgg cactcgcatc acatagcgca gtatcgcggt gccgaccagg ttcgtcgccg
     8821 ccagcatcgc gcgcagccgt gcgtcctctc cgccaccgag cgcgccggac accgcagtga
     8881 gcacgtagtt ctgcatgaag ccgcggaatg cgtcgtgcgc gtcggcgttc gatgtcgccg
     8941 actgcagcat cgacaccatg ctcgccgcgg tgtcgggttg ctcccagatc cgcaggtacg
     9001 cgcgcgccat ccggttcccg atgtcaccgt cgccgtgcgc gatcgcggcc gccagtacgt
     9061 ccgggtcgag gatcagccgc atcgactccc ggaacagttc ggccttcgat ccgaacaggt
     9121 acagcaccat cgacgcgtcg atgtgggctt cgtgtgcgat cgcccgcagc gtcgtcttgt
     9181 cgtagccgtg tctcgcgaac tgccgcttgg ccgcccgcaa caccgcgtcg cgcgacaccg
     9241 gctcgccttg gcgtcgcccg cgccgcttgt tgatcgtcgc gggtcgggac atatctggac
     9301 gctaccgcga cgtctacggc gggatctcct tcgggcggcc cggcccgacc ctgctcatcg
     9361 cgaccggcgc cagccctacc gtcgcccagc tgttcaccca agtaggcaac ggtgtcgccc
     9421 agcacgccgg cgtcgcgttg cgcgtcgaag accttgcacc gccgaccgcc gacgatccgc
     9481 gcggcgcggg gcttgccgcg tcggtgctgc cgatcacgct ggcaggtttg ctgccggcca
     9541 ttgccctcgt gctgctgctg aaacacgagg tgtggaccag attggttgcc actgttgcct
     9601 tttcggcgct atcggcggtg acgatcaccg cgatcctgcg ctacgtgctc ggatcgatca
     9661 atcagaactt ctggggcgtc gcgggcggac tgacgctcgg catactggct gcgggcctgg
     9721 cgattttggg gctcggctcg ctgttcgggc gcgtcgggct ggcggtcggg gcgctggtgg
     9781 cgctgctgct gggcaacccg ctgtccggcc tcaacagtgc gccggagatg ttgcccagcg
     9841 ggtggggcgc attcggccag tacctgccgc aaggcgccaa cgccactttg ctgcggtcga
     9901 cggcgttctt cggcggtgcc ggcgcgggca ccgcggtcac tgtgttgacg tgctgggtgg
     9961 tagccggagc ggtgatcgtt gcggtcgccg cattcagggg ccgcgccggt ggcggcgatc
    10021 ctcatcgtga tcggtttgct ggtgccgtac aaggagatcc acgcgctgaa gagagtccac
    10081 agacacccta gaatcatccg tcgtgggcct ggaggatcgc gacgcgctgc gcttactgcg
    10141 ttcggccctc gatccccccg acgccgacaa cgaactggtc tgcaggtttt acacgcggtg
    10201 gttcgccatc gatccttcga tccgcgacct gtttccgccc gacatggacg gccagcgcgc
    10261 ggcgttcggc gaagcgctgc ggtgggtcat gggtgaactc gtcgcgcggc ggtccgatga
    10321 acctgtcgcg ttcttggctc agctgggccg cgatcaccgc aaatacggtg tcacacaaca
    10381 gcattaccag accatgcagg aagcgctgta cagcagcttc cgcagtcacc tgatcgactg
    10441 ctgggacgac gcggtcgacg aagcggcccg cgatgcgctt gcgttgatca tcggcgtgat
    10501 gagcggcgcc gccgacgccg aatccggccc ggcgtggtgg gacggcacgg tcgtcgaaca
    10561 ccagcgggtg tcgcgcgacg tcgcggtggt tcggcttcag ctggaccggc cgatgcacta
    10621 ctacgcgggg cagtacgtca acgtccaggt tccgcagtgc ccgcgccgct ggcgctacct
    10681 cagcccggcg atccctgccg atccgggcgg cacgatcgag ttccacgtcc gcaccgtgct
    10741 cggcgggatg gtcagcagcg cggtcatcag tgaaacccgc ctcgggcacc gatggcggct
    10801 gtccgctccg cacggcgcga tggaggtcga tcgcgagggc ggcgacgtgc tgatggtggc
    10861 cggcagtacc gggttggcgc cgctccgagc gctgatcatg gacatgaccc gcttcgcgca
    10921 caacccacgc gttcacctgt tcttcggggc gcgctacccc tgtgagctgt acgatctgcg
    10981 cacgctgtgg agcatcgccg cccacaatcc gtggttgtcg gtgtcgccgg tgtcggagta
    11041 caaccacaac ccgccgtggg ccggcgacta ccccgacgtc cagccgccgc gcggccttca
    11101 tgtgcgccag accggtcggc tgcccgaggt ggtgacgaag tacggtgcct ggggcgaccg
    11161 ccagatcctc atcagcggta acccccagat gatcgcaaac accaaggcag cgcttgttgc
    11221 caaaggtgct ccgctggaac gcatccagca cgatccgctc gtgccctgac atcagccgcg
    11281 cgggcggcag gtgagccacc ttgtggcaca atccgacgat ggccgactta gagaccatca
    11341 cgctcaaaga tccgtcctca tccctgaccg caacctacgt accgcgcgcc ggcatggtcg
    11401 gcgcatctct gtcagacgac ggagtcgagc tgctcggtca gcggcgcggc ctgcacgcct
    11461 acatgaccac gggtaagaca atgggcatcc cgatcctgta cccgtgggca aatcggttga
    11521 gcgccaacac atatcaagtc ggcaccgggg cggtgacact gacgcctggc atcggcgggg
    11581 tccgcacgga tcaacacggc gtggtgatcc acggcatcct cgccgcctcc gagggctggg
    11641 aagtcaaaga ccagaccgag aaccggctta ccgccgagct cgactacgga ggcactccgc
    11701 ggctgctggt cagcttcccg ttcccgcatg tgttgaccgt ggacgtcacg cttgccgacc
    11761 gggtgctcac cgtgcgcacc accgtgacgg cgacgaccag ggccgacgtg ccgttgtgct
    11821 tcggcttcca cccgtatctg cggattgccg acgtcccccg ggccgagtgg gagatggagc
    11881 tgcccgccat gcgctactgc ccggtcaacg cgtggatgat cccgaccggc gagacggagg
    11941 accgccccgc atccacagag acgttgggcg acaactactt cgacaacagc tatgacgagg
    12001 tgaagcccgg gtcggtgttc gcggtatcgg gtggcgaccg gcgcatcgag gtgcacttcg
    12061 acgagggata caccgccgcg caggtcttct cgcccagcga cgacgaggtg atctgcttcg
    12121 agccgatgtg cgcccccacc gattcgctac gccgcggcac ctaccgcgtc gccaccgagg
    12181 gggagccggc caccgcgacg ttctcgatcc acgtctagcg cgctagccgg ggtgccgcga
    12241 ctggcgaccc cccgtggtcg ccagtcgtca tcaaatgatg acagcgtgcc cgatataggt
    12301 cactgaactc gatcaactgc gcgttctgct cttcgcgcga gcgctcatcg ccgagagcgc
    12361 cgcgactgcc gcggctccca gaccaccagc gcggtggact tcggcaccac cgcgacgtcg
    12421 cggcgctggt tggtgcgcag ttgctggacc tcctcggtga gctcacgcag ccgcgactgc
    12481 agcgcctcca cctggttggt cagctcgatg atccgcttga tgccggcgag gttgacgccc
    12541 tcatcctgcg acagccgctg cacctcccgg agcaggtcga cgtcacgttg cgagtagcgg
    12601 cggccgccac ccatggtgcg gcgcggcgtg accagaccga gccggtcgta ggtgcgcagc
    12661 gtctgcgcgt gcatgccggc caattgggcg gccaccgaga tcaggaacgt gcgggtctcg
    12721 tcagcgctca taagcgcccc gcccatccgg accttgggtc gaacccgctg gccttctccg
    12781 cggccgcgta cttctccagc gcttccagag cggcgccctc gaggttcggc ggcaccgcca
    12841 ccttcaccgt gaccagcagg tcgccgttgc tgccgtcacg cttgggcaca ccccgtccac
    12901 gcacgcgcag gatgcgcccg tcggccgtgc ccttcggcac ccgcacgccg accttgccat
    12961 ccagtgttgg cacggaaagc gttgtgccta aagctaattc gacgaagctg accggcacgg
    13021 tgacggtcag gtcgtcgccg tcgcggccga acaccttgtc gggtctgacg tgcacggtga
    13081 cgtagaggtc gcccgacggc gccccgcgca gccccgcttc gccctggccg ggcagccgaa
    13141 tccgctgccc gtcctgcacg cccggcggga tgcggacgtt gatggtgcgg gtgcgggtgg
    13201 ccaccccggt gcctttgcag tccgggcagg ggtgctcgat gatcgagccg gtgccgcggc
    13261 agtccgtgca cggttcggag aacccgaacg cgccctggtt gcggttgatc acgcccgacc
    13321 cgttgcagga gctgcacacc ttagggctgg tgcccggccg tgcgccgctg ccgtggcagt
    13381 tcgtgcacgg cgccgggctg gtgagccgca gtggcatcgc gacgcccttg gtggcctcga
    13441 cgaagtcgag ctcggtctcg gtctcgaggt cgttgccgcg acgcggacgg ctgggccgcg
    13501 gctgggcacc acggccgaac aacccgccga acaggtcgcc gatgttcgcg ccgccggtct
    13561 gcccggcgga gtcgaacaga tcgttcaggt tgaactcggt gccgttgccg ccggtgccga
    13621 acccgccgaa gttgccggcc gagaaccggc gacggccgaa gccgccctcg gcgaacagtc
    13681 gccgggtctc gtcgtactcc ttgcgcttgg ccggatccga cagaacctcc ttggcttccg
    13741 cgacagcctt gtaccgttct tcggctgctg cattgcccgg gttgcggtcg ggatgattct
    13801 cggcgagtag cttgcgggcc acccgcttga tctcatcttc gctggcatca gaggagacgc
    13861 ccagctcctt gtagaagtcc ttttcgaccc attcgcgttg agccatgcgg cgtcacctcc
    13921 ttcccaggat cagctggctg attcttctga ttctgcggtg tcttccgcat ccgcgtcggc
    13981 ttcttcacct gttgctcttc gagcaagcgg ctcatcgtcg accgtgtcga ccacaccgac
    14041 cagcgcgtgc cgcaagacct ggtcacccag ccggtagccg cgtcgcatca ccgttccgac
    14101 gaccggcttg gtgccgtccc cctcgtgctg aaccgcttcg tgcagtgacg ggtcgaagtc
    14161 gtcgccctcg tcgccgaacg ccgtcaggcc gagtccctcc atcgcgccgg tcagcttgtc
    14221 cgcgaccgac ttcaacggcc cggattcgag gtcgccgtgg ttgcgggcgc ggtcgagatc
    14281 gtcgagcaca ccgagcaatt gggcgacgac ggccgccttc gcccgttccg cggcgagctg
    14341 ttcctgacgg accgagcgct tgcggtagtt gtcgtactcg gccttgaccc gctgcagcgt
    14401 ggccttcagc tcggcggcct cttcggtgtc gggcacaccc ggtgccggcc cactcggggc
    14461 cgggccggct gctccctcac gcacttcccc cgtttcgggg tcgatgcgcc gcttgtcggt
    14521 gatggtgacg ggttcgtggc cttcgtcggg cgtcacctgt cctcctgatt ctcgtcgacc
    14581 acctcggcat ccactacgtc gtcgccaccg ggagcgctct cagcgccgtc gccgccggcc
    14641 gcctgggcgg cctgagtggc ctcgtagatg gcctggccca gcgcttgcga ctcttgaccg
    14701 agcttttcca tcgcggactt gatcgcgttg atgtcggtgc cgccgagcgc ggtcttggcg
    14761 tcggcaatcg ccgcgtcgac cttgttcagc gtgtcctcgg ggaccttgct gccgccctcg
    14821 gcctcacgct gttcttcgac gaacttctcc gtctggtaca ccagcgtctc ggcctggttg
    14881 cggacgtcgg cctcctcgcg acggttgcgg tcttcgtcgg cgtgcgcctc ggcgtccttg
    14941 atcatccggt cgatctcctc cttggacagg ccggagccct cctggatttg gatcttgttc
    15001 tccttgccgg tgcccttgtc cttggcggtg acgtgcacga tgccgttggc gtcgatgtcg
    15061 aacgtgacct cgatctgcgg cacgccgcgc ggagccggtg cgatgccggt cagttcgaag
    15121 ctgccgagca gcttgttggc cgaggccatc tcgcgctcac cctgatagac ctggatctgc
    15181 accgacggct ggttgtcgtc ggccgtggtg aaggtctcgc tccgtttggt cgggatcgtg
    15241 gtgttgcgct cgatcagctt ggtcatcacg ccgcccttgg tctcgatgcc cagcgacagc
    15301 ggcgtaacgt caagcagcag aacgtctttc acttcgccct tgagcacacc ggcctgcagc
    15361 gcggcgccca ccgcgacgac ctcgtcgggg ttcacaccct tgttgggctc cttgccgccg
    15421 gtcatttcct tgaccagatc cgacactgcg ggcatccgcg tggagccgcc caccagcacg
    15481 acgtggtcga tgtcggccac cgagatgctg gcgtcggcga tcaccgactg gaacggcttg
    15541 cgggtgcgat ccagcaggtc ctgcgtgatc cgctggaact ccgcgcgcgt cagctgctcg
    15601 tcgaggaaca gcgggttctt gtcggcgtcg acggtgatgt acggcaggtt gatcgaggtg
    15661 ctctggctcg agctcagctc gatctttgcc ttctcggcgg cctcccgcag ccgctgcatc
    15721 gccatcttgt ccttggtcag gtcgatgccg ctggtgcccc tgaacttgtc gaccagccac
    15781 tcgacgatgc ggtcatccca gtcgtcgccg ccgaggtggt tgtcacccga ggtggcgcgg
    15841 acctcgacga cgccctcgcc catctccagc agcgagacgt cgaacgtgcc gccaccgagg
    15901 tcgaacacca ggatcgtctg ctccttgtcc gccttgtcca agccgtaggc cagcgcggcg
    15961 gcggtgggct cgttgatgat ccgcagcacg ttgaggccgg caatctggcc ggcgtccttg
    16021 gtggcttggc gctgcgcgtc gttgaagtac gcgggcgtgg tgatcacggc gtcggtgatg
    16081 tcctcaccga ggtagctttc ggcgtcgcgc ttgagcttca tcaagatgcg cgcgctgatc
    16141 tccggcgcgg tgtagttctt gccgtcgatg tcgacggacc agtcggtgcc catgtggcgc
    16201 ttaaccgacc ggatggtccg gtcgacgttg gtcaccgcct ggttcttggc gggctgaccc
    16261 accaggacct cgccgttgcg cgcgaatgcg acgacggacg gcgtggtgcg ggagccctcg
    16321 gagttggcga cgacgacggg gtcgccaccc tccagaaccg cgacgacgga gttggtggtc
    16381 ccgaggtcga ttccgaccgc acgagccatg gtgtttcctc ctgaactgat atgtgtacgt
    16441 ctgctgagcg aatcaggctc aagtctgctc cgctggcgca gtccgcgtca actcgtagtt
    16501 gagcctagtt cactcaagtt gtcgaacatg ccaacggggt gggcggcgag tttgttcccg
    16561 gacctgcgcc gaatagcagg cgttcctacc tcagatcccg aagacccggc gcgcgttgcc
    16621 cgcgagatag aggcttcggg cctcgtcgct cagcccgagg tcgtcgagtc cgtcgagggc
    16681 gtgggccggc gcgatcatcg ggtagttgct tccgaacaac accttgtgct gcccgctgcc
    16741 cgtcttgagg taggtcacca gttctggcgg cagccgtttg gtcgtatacg ccgacgtgtc
    16801 gatgacgacg ttgtcgtgct tgcgggcgac ggcgatcatc tcctcggtcc acggatagcc
    16861 gacgtgcccg cagacgatcg tcagctccgg gaagtccagc gccacctggt cgatgtacgg
    16921 aatcgggcgg cccgtctcgg acgggcgcag cggaccggtg tggccgacct gggtgcagaa
    16981 cggcacaccc aattgcacac attcggcata cagcgggtag tagcgcctgt cggtcggggg
    17041 cgcgttccac aaccacggca ccacccgtaa acccttgaac cccaactccg tgacgcagcg
    17101 ccgcaactcg cgcacggccg acatcggctt gtcgaggtcg acgcaggcca ggccggccaa
    17161 ccgatctgga tgggccgcaa cccaaccggc gacctcgtcg ttgctgatca acggttcctg
    17221 gtgaggcgcc gaccacgccg acagcagccc gaagcccaca ccggcggcgt ccatcgcatc
    17281 aacggtcgct tcgatgccgg gcatctggtc ggggatggaa tcaccggtcc atcggcgcag
    17341 cgacgcgaag atgtcctggg ccaggaagcg aggcgtcggg tgctgcatcc acacatcgat
    17401 gaccgtcacg gttcgagtgt gcgcctcacc tgtcggacgg gctcggcgtt cgcggcctta
    17461 ggcggcgacg gtcacgtcga cggacagcag atcctcgagc gcgtgggcgg cggtccagtt
    17521 gccggtgcgc acggcttgcg cgagttcgtc gaccatcggg gtctcgacct ccagctcggc
    17581 cagccacgcc gacacggtgg ccgcgatctc gttgctgctc acccgcgcgg cacgtcccct
    17641 gtgcagccgg tccgcaacct ggtaaacggt ggccgtgttg cgcgatgtcc gccgaatcca
    17701 gttggtgctc atgacctgct ccttgtccgt tcttggcggc ttgtgtgccg ctgtcatggg
    17761 taggagtgag aagccctgtc ggcagtgacg ttttttcaga ggacttttcc aaacctcttt
    17821 cgtcacgtga gtcactacag tcacgttcgt cactgcttaa gtaccctgat agagatgcgc
    17881 gagggggaag cggtgcggaa atcggcggtg gccctgatgg ccgcagcgat agcgctcacc
    17941 ggctgcacgt cgtggacgaa cggcaagccc gtcgccgtcc cgtccaaccc gaccgagccg
    18001 acgttcccga cgacccgccc gagccgcacc ccgccgagca cgccgtcgtc caccacgccc
    18061 gttccggcgc ccacgccgcc accgggcggc gagacgctcg acccgaatga gcagggctac
    18121 gtgtacatcg aaaccaagtc cggcaagacc cgctgccaaa tctctcagac ggccgtgggc
    18181 tgtgaggcac cgttcaccaa ttcgccgatc cagggcggta ttcccgcgaa cggagtcgac
    18241 gtcaccgcgg gcggcgattt ccggtggctg gtcggcaacc tcggcgacat cacacccgtg
    18301 accctcgact accgcaccta ccaggcgcag ggctggacaa tcgcggcgga cgaagacggc
    18361 acccgcttca ccaacaaccg cagcggccac gggatgttcg tcagcatcga gaaggtcgag
    18421 tccttctagc acactaaacg tgctggtcag ggcaccgatc gaggctcagc gaacggttct
    18481 ggaccgcaga tggtccgcag tagatcggat cgcggtgtcc agccggtccg caacgccgtc
    18541 cagatcctcg tcgaacagat cggcgtagac gtccagcgtc atggacgcct tggcgtggcc
    18601 gagcatgcgc tgaacggcct tcacattcgc cccggcgctg atggccaggc tcgccgccgt
    18661 atgccgtagg tcatgcggcg tgatggacgg gaaggcgtcg tcgtcggcct ggcacttctg
    18721 cactgccgac gcaaaggtcc ggttacggta gttcgagtgg cgcagcacgc cgccgcgtga
    18781 gtcggcgaac accaggtcgt cacggccctt gcccatcatg agcgcggcca gctcgtcggc
    18841 cagcgatgcg cgtgcgcttc gacatcacgc tggagcccag cgcacctctg ttcgaattcc
    18901 tgatcaagcg gggcgagacg atcgtgctcg acgccgcgac ggagggccta ggcacgcgtg
    18961 tcctcggcta gccgactggc ataggcatcg gacttgaaac ttcacaactg ctgtgggcgt
    19021 cggcgcgccg acccgggtca gccgaagttc tggccaccat tgggctcggc caacaccgcc
    19081 cagcgcggag gtcaatcgct ttaggtcgcg ctccacacgg ctagccgtcg gcgggctcgt
    19141 ccaggccgct gtgcccgtgg cagtccagaa cagcacaccg ccgccgttgg ggcgtaacct
    19201 ccgtctgatc cttctgaccg tcataatgcg tcagagtctg cgaaacgagc tccgtttcgc
    19261 cgccgcagat cgggcagcga tggtcagcgt cacgtacgat tcgtggctct atcggcatgt
    19321 ccatgtcccg caccctaaac cctggtaggt cttggaacga gtggcccaaa gccctccggc
    19381 gtggggtagt tcttccctac ttgcttctgt cgcatcggag ttaaatggtc gcagtctagg
    19441 atcatccgat ccaatatcaa ctctgggagg tcatcgtgtc aggcaccgtc gctcgcgtca
    19501 ccgagatcag cgccaggtcc gacaccagtt tcgaagacgc cgcgaaaggc ggcctcgctc
    19561 gggccaacga gacgctgcgt ggcgtcaagt cggcctggat caaggagcag tcggtggaag
    19621 tggagaacgg aaagattgtt gcctaccgcg tcaacatgct ggtgacgttc gtcctcgact
    19681 agcgggcgaa acccggggac gcggtcccac gcagaggcac gaatggcccg gcctgggggt
    19741 attccctggg gcgggccgtt ctgttggggc cacacggggg gtgaaggtgc gcagcgcctc
    19801 cagcagccgc tgcagccaac ggtggccatc gggaccggct gcccgccatc cagaccgatc
    19861 tcggtgaccg gaccatcacg gggaacacgt caaagcactg ccactccagg gggtccggga
    19921 gatcggctcg cccagtgcgt tcgtggccga tctgtacggg gcgcacttgc acaggcacct
    19981 gggcagtcgc cctttgaggt gtggtggcga ttgcctcagg cagattggga tgcggacggg
    20041 gtttcgggtt cacgcggccc catgtcgagg cggaacggtg gatactcgtc gcgcatcagc
    20101 gaggtgtagg cgaatacccg gatggcccac cggttgatgc cgatcacgaa gtcgtagagg
    20161 cccttcgggt agcgcgcggt gaacagcagg gccaccaccg cgatcagcag caacacgcgg
    20221 atcagcggca ccaagatggt gccgtcaccc cagtcgtcgt cgtcattgcc catgaaggcc
    20281 ccgacaccgc cgtagaagaa ggccaccagt accaggtagt gcgggacggc cagcaaccac
    20341 cacttgatca gcaccagacc ccgatgtag
//
LOCUS       DASWBF010000073         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1056276,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000073 DASWBF010000000
VERSION     DASWBF010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056276"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VF874_02135"
     CDS             complement(<1..1032)
                     /locus_tag="VF874_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9174923.1"
                     /translation="MTTTENRLTADVRNGIDYKVADLSLAEFGRKEIRLAEHEMPGLM
                     ALRREYAGVLPLKGARISGSLHMTVQTAVLIETLVALGAQVRWASCNIFSTQDHAAAA
                     VVIGPHGTAEEPQGVPVFAWKGETLEEYWWATEQMRTWPGEPVNMILDDGGDATMLVL
                     RGAEYEKAGVVPPGEDDDPAEWKVFLARVRERFESDKTKWTKIAESVKGVTEETTTGV
                     LRLYQFAAAGELAFPAINVNDSVTKSKFDNKYGTRHSLIDGINRGTDVLIGGKNVLIC
                     GFGDVGKGCAESLKGQGARVAVTEIDPINALQALMEGFDVKTVEDAIGDADIVITATG
                     NKDIITLEHM"
     gene            complement(1076..1690)
                     /locus_tag="VF874_02140"
     CDS             complement(1076..1690)
                     /locus_tag="VF874_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9174924.1"
                     /translation="METQQRVPYAEASRALLRDSILDGMRDLLLTRDWSAITLADVAR
                     AAGISRQTIYNEFGSRLGLAQRYALRLADRLVDVIDDQIRRNEGDIYSAFVQGFRAFF
                     AESASDPLVISLLNGEAKPDLLQLITVDSAPIINHCSQRLTASFAQSWVNASDEDAGV
                     LARAIVRLAMSYVSMPPEADHDVADDLARLMTPFAERYGVIEQN"
     gene            complement(1708..>1863)
                     /locus_tag="VF874_02145"
     CDS             complement(1708..>1863)
                     /locus_tag="VF874_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX9174925.1"
                     /translation="IQCGFAYDEALGWPEDGIAAGTRWDDIPDDWSCPDCGAAKADFE
                     MIEVARS"
BASE COUNT          279 a          657 c          592 g          335 t
ORIGIN      
        1 catgtgctcg agcgtgatga tgtccttgtt gccggtcgcg gtgatcacga tgtcggcgtc
       61 gccgatggcg tcctcgacgg tcttcacgtc gaagccctcc atcagcgcct gcagcgcgtt
      121 gatcgggtcg atctcggtga cggcgacgcg cgccccctga cccttgagcg actccgcgca
      181 gcccttgccg acgtccccga agccgcagat cagcacgttc ttgccgccga tcaacacgtc
      241 ggtgccacgg ttgatgccgt cgatcagcga gtgccgggtg ccgtacttgt tgtcgaactt
      301 ggacttggtc accgagtcgt tgacgttgat ggccgggaag gccagctcac cggccgcggc
      361 gaactggtac agccgcagca ccccggtggt ggtctcctcg gtgacgccct tgaccgactc
      421 ggcgatcttc gtccacttgg tcttgtcgga ctcgaagcgt tcccgcacgc gggccaggaa
      481 caccttccac tcggccggat cgtcgtcttc gcccggcggg acgacgccgg ccttctcgta
      541 ctcggcgccg cgcagcacca gcatcgtcgc gtcgccgccg tcgtcgagaa tcatgttcac
      601 gggctcgccg ggccacgtcc gcatctgctc ggtggcccac cagtactcct cgagcgtctc
      661 gcccttccag gcgaacaccg ggaccccctg cggctcctcc gccgttccgt gcgggccgat
      721 gacgacagcc gccgcggcgt ggtcctgggt ggagaagatg ttgcacgacg cccagcgcac
      781 ctgcgcgccg agcgcgacca gcgtctcgat cagcaccgcc gtctgcacgg tcatgtgcag
      841 cgaacccgag atgcgggcgc ccttgagcgg cagcacccct gcgtattcgc gccgcagcgc
      901 catcaggccc ggcatttcgt gttcggccag ccggatttcc ttgcgaccga actcggccag
      961 cgacaggtcg gccaccttgt agtcgatgcc gtttcggaca tcagcggtca gcctgttctc
     1021 agtcgtcgtc atttcgtcct tcttgtgcac gcgcggcgat cacttcgcca ggccgctaat
     1081 tctgttcgat gacaccgtat cgttcggcga acggcgtcat cagcctggct aggtcgtcgg
     1141 ccacatcgtg atcggcctcc ggcggcatcg acacgtagct catcgccagc cgcacgatcg
     1201 cgcgcgccag cacgccggcg tcctcgtcgg aggcattcac ccagctctgc gcgaacgacg
     1261 cggtgagtcg ctgcgagcag tggttgatga tcggcgcgct gtcgacggtg atcagctgca
     1321 gcaggtccgg cttggcctcg ccgttgagca gggagatcac cagcgggtcc gacgccgact
     1381 cggcgaagaa ggcccggaac ccctgaacga aggcggagta gatgtcgcct tcatttcggc
     1441 ggatctggtc gtcaatcacg tcgacgaggc ggtcggccag ccgcagcgcg tacctctgcg
     1501 ccaggcccaa ccgcgagccg aactcgttgt agatcgtctg gcggctgatc ccggcggcgc
     1561 gtgcgacgtc cgccagcgtg atcgccgacc agtcccgcgt cagcagcaga tcccgcatgc
     1621 cgtcgaggat cgagtcgcga agcagcgctc gcgacgcctc ggcatagggg acccgctgct
     1681 gtgtttccat acgggcgaca ctaaccgtca cgaacgggcg acctcgatca tctcgaaatc
     1741 cgccttggcc gcgccacagt ccgggcagct ccagtcgtcg gggatgtcgt cccagcgcgt
     1801 gccggcggcg atgccgtcct cgggccagcc cagcgcctcg tcgtacgcga agccgcactg
     1861 gat
//
LOCUS       DASWBF010000074         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1056281,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000074 DASWBF010000000
VERSION     DASWBF010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056281"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VF874_02150"
     CDS             complement(<1..739)
                     /locus_tag="VF874_02150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX9174926.1"
                     /translation="MADTALVVGEALIDIVEHQGQTLGEHVGGSPLNVAVGLALLARR
                     VEFLTWIGDDPRGRRIADYLHDSGVHLAAGSIGASRTATALAELDEKGSATYTFDINW
                     QVSPSVSTTEPLVLHTGSIATALMPGASGVVELVDKHKPTATITFDPNIRSAFYENPG
                     QALGRVEAVVARSDVVKASDEDMRWLDPDSTPEELAARWLALGPAIVAVTFGADGSYA
                     ICTDGAQRIPAYPAEVVDTVGAGDSFMT"
     gene            complement(760..1146)
                     /locus_tag="VF874_02155"
     CDS             complement(760..1146)
                     /locus_tag="VF874_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEX9174927.1"
                     /translation="MADRNVLGGPLEPCGTDPLTGFYRDGCCSTGPEDIGLHTICAVM
                     TAEFLQHQRSIGNDLSTPLPQYRFPGLVPGDRWCVTAANWLRSHHDGCAAPVVLASTH
                     ESTLDVVALEALQQHAVDVPDDPGEL"
     gene            complement(1350..>2223)
                     /locus_tag="VF874_02160"
     CDS             complement(1350..>2223)
                     /locus_tag="VF874_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX9174928.1"
                     /translation="AYPFISTVPTFFSELRTANWWWALLGLAVSALTYLGAAAALWAC
                     ANGLVSFKNLAIMQVANTFAATTTPAGIGGLALSTRFLRKAGLGTVRATAAVALQQTV
                     QVLTHVVLLIFFSAAVGASADLSHFVPSATILYLVAGLALGAVGAFLFVPRLRRWLGT
                     EVRPRLTEVGGDLVELVREPKRLALIVLGCAATTLGMALALWASVEAFGGGTTFVTVT
                     VVTMIGGTLASAAPTPGGVGAVEAALIGGLAAFGVPAAVGVPSVLLYRVLTCWLPVFV
                     GWPIMRWLTRNEMI"
BASE COUNT          381 a          741 c          772 g          329 t
ORIGIN      
        1 cggtcatgaa cgagtcgccg gcacccaccg tgtcgacgac ctcggccggg tacgccggta
       61 tgcgttgcgc gccgtcggtg cagatggcgt aggagccatc cgctccgaaa gtgactgcga
      121 cgatcgcggg cccgagcgcc agccagcgcg cggcgagctc ctcgggcgtc gaatcgggat
      181 cgagccagcg catgtcctcg tcgctggcct tcaccacatc cgagcgggcg acgacggctt
      241 ctacgcggcc cagtgcctgc ccggggttct cgtagaacgc ggaccggatg ttggggtcga
      301 aggtgatggt cgccgtcggt ttgtgcttgt cgaccagctc gaccacgccg ctggcgcctg
      361 gcatgagggc ggtggctatc gaaccggtgt gcagcaccaa gggttctgtg gtgctgacgc
      421 tcggcgatac ctgccagtta atgtcgaaag tgtaagtcgc cgagcctttt tcatcgagct
      481 ctgccaacgc ggtggccgtg cgagatgccc cgatgctgcc cgcggcgagg tgcacgccgc
      541 tgtcgtgcag gtagtcggcg atccgccggc cgcgggggtc gtcgccgatc catgtcagaa
      601 actcgacgcg acgagcgagc agcgccaggc cgaccgcgac gttcagcggg ctgccgccga
      661 cgtgctcccc gagcgtctga ccctggtgct cgacgatgtc gatcaacgct tcgcccacga
      721 caagagcggt atcggccatc gcactccttc gactgctcgt catagctcgc ctggatcgtc
      781 gggcacgtcg acggcgtgct gctgcagcgc ctccagggcc accacgtcga gagtggactc
      841 gtgggtggac gccagcacca ccggggcggc gcagccgtcg tggtgggacc gcagccagtt
      901 cgcggcggtg acacaccagc ggtcgccggg cacgaggccg ggaaaccggt actggggcag
      961 cggtgtcgac aggtcgttgc cgatcgagcg ctggtgctgc aggaactcgg cggtcatgac
     1021 ggcgcagatg gtgtgcaggc cgatgtcctc cggcccggtc gagcagcagc cgtcgcggta
     1081 gaagcccgtg agcggatcgg tgccgcacgg ttccagcggg ccaccgagca cgttgcggtc
     1141 ggccatcggc tcagtatgcc cggatatctg ctggccgacg ggtgcgaaat ggcggaattt
     1201 gtcgctgcga ggcgggcagt tcgactagtg ccttcctcgg tggcatctgg gctagggtga
     1261 caatcgttac cacacgagcc tcagatgtca cagtttcccc ttcgaggacg ggatatgttc
     1321 gtgcccgact ctgagcgaca acggcgccgt cagatcatct cgttcctcgt cagccaccgc
     1381 atgatcggcc acccgacgaa caccggcagc cagcaggtga gcacccggta caacagcacc
     1441 gacggcacgc caacggccgc gggcaccccg aacgccgcca gcccgccgat cagcgccgct
     1501 tcgacagctc cgacgccacc gggagtgggc gccgccgaag cgagggtgcc gccgatcatc
     1561 gtcacgacgg tgacggtgac gaacgtggtg ccgccgccga acgcctcgac gctggcccac
     1621 aatgccagcg ccatgccgag agtcgttgcg gcacaaccga gtacgatcaa cgccagccgc
     1681 ttgggttcgc ggacgagctc gacgaggtcg ccaccgacct ccgtgagccg aggccgcacc
     1741 tcggtcccga gccagcgccg caaccgaggg acgaacagga acgcgccgac agccccgagc
     1801 gctagcccgg cgaccaggta caggatcgtc gccgacggga cgaagtggga cagatcggcc
     1861 gacgcgccca cggcggcact gaagaagatc agcaggacca cgtgggtgag cacctgcacc
     1921 gtctgctgga gtgcgacggc cgcggtcgcg cgcacggtac ccaggccggc cttccgcagg
     1981 aatcgggtgc tcagcgcaag cccgccgata cccgccgggg tggtggtggc ggcaaaggta
     2041 ttggccacct gcatgattgc gagattcttg aagctcacca atccgttggc gcaggcccac
     2101 agcgcggccg ccgcgccgag gtaggtgagt gccgacaccg ctaagccgag cagcgcccac
     2161 caccagttcg cggtgcgcag ttcggagaag aacgtcggca cggtgctgat gaacgggtag
     2221 gcg
//
LOCUS       DASWBF010000075         8751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_729009,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000075 DASWBF010000000
VERSION     DASWBF010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729009"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VF874_02165"
     CDS             complement(<1..439)
                     /locus_tag="VF874_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system glycosyltransferase"
                     /protein_id="HEX9174929.1"
                     /translation="MTQPRLPDGFAVQVDRRVRVLGSGAALLGGSPTRLLRLAPSART
                     LIDNGRVEVHDAVSAQLARTLLDATVAHPRPSGGPSHRDVTIVVPVHDNMFGLRRLLS
                     TLRGLRVIVVDDGSAIPVRQSDFEPMSCQVEVIRHPHTKGPAAA"
     gene            complement(436..1170)
                     /gene="mftE"
                     /locus_tag="VF874_02170"
     CDS             complement(436..1170)
                     /gene="mftE"
                     /locus_tag="VF874_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="HEX9174930.1"
                     /translation="MNSAYDRHVVFPTDLGKSTSKQVEGVSPTVVVPVGSTEQHGPHL
                     PLDTDTRIADAVAHAVVPTFPPGSATVAPAIAYGASGEHQAFAGTLSIGTAALETVLV
                     EFGRSACAWAPRVVFVNGHGGNVDALVAAVRRLRDEGCDAAWCPCSVPGGDAHAGHTE
                     TSILLHLSPQTVRIDERRTGNVAPLAELMSTMRRGGVAAVSEVGVLGDPTTATAEAGA
                     RLFTEMVDACTTWIRCWAPGDDGMLR"
     gene            complement(1293..2480)
                     /gene="mftD"
                     /locus_tag="VF874_02175"
     CDS             complement(1293..2480)
                     /gene="mftD"
                     /locus_tag="VF874_02175"
                     /EC_number="1.4.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209158.1"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the
                     non-exchangeable NADH of TIGR03971 family SDR-type
                     oxidoreductases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="HEX9174931.1"
                     /translation="MAQWFETVAFAQEKAKRRLPKPVYAALLAASERGVTVADNIDAF
                     SELGFAPHVIGATDKRDMTTTVLGQDISLPVVISPTGVQAVHPDGEVAVARAAAARGT
                     AMGLSAFASKPVEDVIAVNEKVFFQVYWLGDREAIARRVERAREAGAVGLILTTDWSF
                     SHGRDWGSPKIPEQMNLRTTVRMMPTGLTKPVWLWEWAKTLRPPDLRVPNQAPRGEPG
                     PPFFAAYGEWMNTPPPTWEDVAWLRELWGGRFMLKGVMRVDDAKRAVDVGVSAISVSN
                     HGGNNLDGTPASIRALPAIVDAVGDDIEVLLDGGIRRGSDVVKAVALGARAVMIGRAY
                     LWGLAAGGQAGVENVLDILRGGIDSAMRGLGKSSIHDLTPEDIVMPPDFIRALGVPTD
                     RSS"
     gene            complement(2483..3643)
                     /gene="mftC"
                     /locus_tag="VF874_02180"
     CDS             complement(2483..3643)
                     /gene="mftC"
                     /locus_tag="VF874_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726082.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HEX9174932.1"
                     /translation="MTAVPRLVEQFERGLDAPICLTWEQTYACNLACVHCLSSSGRRD
                     PRELTTRQCMDVIDELERMQVFYVNIGGGEPTVRPDFWELVDYATTHHVGVKFSTNGV
                     RITPARAERLAASDYVDVQISLDGATADVNDAVRGAGSYDMAVRALDHLAAAGFTDAK
                     ISVVVTRHNVDQLDEFAGLATRYGATLRITRLRPSGRGADVWDELHPTAEQQVQLYNW
                     LVDRGDRVLTGDSFFHLSPLGQPGALAGLNLCGAGRVVCLIDPVGDVYACPFAIHDRF
                     LAGNVLSDGGFDNVWKNAPLFRELREPQSAGACSSCGHYDSCRGGCMAAKFFTGLPID
                     GPDPECVQGFGAPALARERHVPRPSVDHSRRGPVALTLTSRPPTRLCNESPV"
     gene            complement(3640..3912)
                     /gene="mftB"
                     /locus_tag="VF874_02185"
     CDS             complement(3640..3912)
                     /gene="mftB"
                     /locus_tag="VF874_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167823.1"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HEX9174933.1"
                     /translation="MFDATRGWRLHPQVAVRPEPFGALLYHFGTRKLSFLKDRTILAI
                     VQSLGEYPDAQSACRAAGVTAGGERPYLHALEVLATSDMLVGQERG"
     gene            complement(3914..4039)
                     /gene="mftA"
                     /locus_tag="VF874_02190"
     CDS             complement(3914..4039)
                     /gene="mftA"
                     /locus_tag="VF874_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245093.1"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HEX9174934.1"
                     /translation="MEPNSQAQAAGAAASATSGTEELVTETLVEEVSIDGMCGVY"
     gene            4133..4723
                     /gene="mftR"
                     /locus_tag="VF874_02195"
     CDS             4133..4723
                     /gene="mftR"
                     /locus_tag="VF874_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881927.1"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from
                     the terminal Val-Tyr dipeptide of the precursor peptide
                     MftA, through a radical SAM enzyme-mediated process;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="HEX9174935.1"
                     /translation="MVQPRVGRRRSTSRHQITDVAIALFAGRGFGEVSVDDVAEAAGI
                     ARRTLFRYYPSKNAIPWGDFDAHLQHLWRLLAAVDPAVPIEQALRDALSAFNTFDESE
                     TSRHRLRMQVILRTTELQAYSMTMYAGWREVIAEFVAARCGTKPDDLVPQTVAWALLA
                     VSLSAYEHWLADESVSLTQALADAFDVIRPGRELLT"
     gene            4848..5360
                     /locus_tag="VF874_02200"
     CDS             4848..5360
                     /locus_tag="VF874_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9174936.1"
                     /translation="MSAEPRPPDAPRALLALYDQALPAVYGYFARRCGDRSTAEDLTS
                     ETFLTAMDSARKDHPPDVSVPWLIGVARHKLADHFRRRPDRRAVPVAEVPEDPDDIWD
                     AELDRIVADSTLRRLSEPHRAVLTLRYMDDCSVNECAELIGRTVHATEALLVRARRAF
                     RHHYEEGGAR"
     gene            5357..6652
                     /locus_tag="VF874_02205"
     CDS             5357..6652
                     /locus_tag="VF874_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9174937.1"
                     /translation="MTHDPLNVLRADDLPVQPDPEFAARLRARLESALSLPAGTEGVE
                     MSGTAQALADLTEAAAPPRPAALPYLAVADARAAIAWYADTLGAVLVGEPIVMDDGRI
                     GHAELALAGGVLYLADEYPEFGLKAPAPQSVSVSLMLHVADTDAALRQARDHGAVVQR
                     EVYEGHGSRNATIVDPFGHRWMLSGPVSAPVEVIRHGDLGYVSVWTPDADRAAAFYGH
                     VLGWTYDPSTHQVTNTQQPVGLFSVATHPTMFCCYAVSDLQAAARAIVAAGGRVGEER
                     EFGVGTVLDATDPTGTAFAVYRAADDARRLPLNGAGPGELSYVTYEVDNSAAFREFYG
                     QVLGWTFEPGSIQDGWQITGTHPMAGVAGGSAEPTTVPMWTVADIDDAVARVREAGGT
                     VMQEPNRQPYGVMAECRDDQGSRFYLGEFSAAPKPNSRS"
     gene            complement(6701..7873)
                     /locus_tag="VF874_02210"
     CDS             complement(6701..7873)
                     /locus_tag="VF874_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9174938.1"
                     /translation="MSKRIVIVGGGLAAARTAEQLRRSGFDGSITIVSDEVHLPYDRP
                     PLSKQVLRSEMDDTALNPREFYDENNITLLLGSAVRKLDTANQTVTLENGTQLEYDEA
                     VIATGLVPRRIPSFPDLRGIHVLRSFDESMALREEAGAARRAVVVGAGFIGCEVAASL
                     RGLGVAVVLVEPQPEPLASVLGEQVGALVARLHRAEGVDVRTGVGVSSVAGSGRVEKV
                     VLTDGSELDTDLVVVGIGSRPATDWLEDSGVKVDNGVVCDHVGKTSAPHIWALGDVAS
                     WQDATGHQARVEHWSNVAEQARVMVPSLLGQELPDNLVVVPYFWSDQYDIKIQCLGEP
                     EADDIVHLVNDDGRKFLAYYERDGVVAGVVGGGMPGAVMKTRTKIAAGAPISEVLN"
     gene            complement(7906..8352)
                     /locus_tag="VF874_02215"
     CDS             complement(7906..8352)
                     /locus_tag="VF874_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9174939.1"
                     /translation="MDRLDDIDERILAELAEHARATFAEIGEKVSLSAPAVKRRVDRM
                     LDRGVIKGFTTVVDRNALGWNTEAYVQVFCQGTIAPERLRAAWVDIPEVVSAATVTGT
                     SDAILHVLARDMQHLEEALERIRSSADIERSESIVVLSNLIDRTRP"
     gene            8476..>8751
                     /locus_tag="VF874_02220"
     CDS             8476..>8751
                     /locus_tag="VF874_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-dimethylarginine dimethylaminohydrolase"
                     /protein_id="HEX9174940.1"
                     /translation="MTEYLFAADDACRDAPSRLHARTPRARRYVMTPPEFFAVEYAIN
                     PWMDVATPVDRQIAARQWESLYETYLRLGHLVELVRPEAGLPDMVYAA"
BASE COUNT         1461 a         3012 c         2834 g         1444 t
ORIGIN      
        1 tcgcggcggc gggtcctttg gtgtgcggat gccggatcac ctccacctga cagctcatgg
       61 gctcgaagtc ggactggcgc accggaattg ccgagccgtc gtcgacgacg atcacgcgca
      121 gccctctcag cgtcgacaac agccgccgta agccaaacat gttgtcgtgc acgggaacaa
      181 cgatcgtgac atcacggtga gacggtccgc cggaaggacg ggggtgagct acggtggcgt
      241 ccagcagcgt ccgtgccagc tgcgcgctga ccgcgtcgtg cacctcaacc cggccgttgt
      301 cgatcagcgt gcgggccgac ggggcaagtc gcagcagccg agtgggcgat ccgccgagca
      361 aggcagcgcc cgagcccagc acgcggacgc gacgatcgac ctggacagca aacccgtccg
      421 gcaaccgcgg ctgggtcatc gcagcatccc gtcgtcaccg ggagcccagc accggatcca
      481 ggtcgtgcag gcgtcgacca tctcggtgaa caggcgcgcg cccgcctccg ctgtcgcggt
      541 ggtggggtcc cccagcactc ctacctcgct cactgccgct accccgccgc ggcgcatggt
      601 cgacatcaac tccgctagcg gagctacgtt acccgtacga cgctcgtcaa tgcggacggt
      661 ttgcggtgaa agatgcagga gtattgaggt ttccgtgtgg cccgcgtgag catccccgcc
      721 gggcaccgag caggggcacc acgcggcgtc gcacccttcg tcccggaggc gcctcaccgc
      781 cgccaccagc gcgtcgacgt ttccgccgtg gccgttgacg aacacgactc gtggtgccca
      841 tgcgcacgcg gagcggccga actccaccag caccgtctcg agagcggcgg ttccgatcga
      901 cagggtcccg gcgaaagcct gatgctcccc gctggccccg taggcaatcg cgggcgcaac
      961 cgtggccgat ccggggggga atgtggggac gacggcgtgc gcgactgcgt cggcgatgcg
     1021 ggtgtcggtg tccagcggca gatggggccc gtgctgctcg gtggagccca ctggaaccac
     1081 cacggttggc gacacacctt cgacctgctt cgacgtcgac ttccctaaat ccgtcgggaa
     1141 gaccacgtgg cgatcgtagg ccgaattcat ctgggataca cgaagtgttc gcggatgagt
     1201 agatttgctt ccgaaaaaac tgcgttccgc cacgaacaga tttcacgtca gcatcgccag
     1261 ccccgtcggc accagggatt ccgaagctga gctcacgaac tccggtcggt cggaaccccc
     1321 agggcgcgga tgaagtcggg aggcatcacg atgtcctccg gcgtgaggtc gtggattgac
     1381 gacttcccga gaccgcgcat tgccgagtcg atgccgccgc gaaggatgtc gaggacgttc
     1441 tcgacgccgg cctggccgcc cgccgcgaga ccccataggt aggcccgacc gatcatcacg
     1501 gcccgagcgc ccagcgccac cgccttgacg acgtcgctgc cgcgccggat gccgccgtcg
     1561 agcagcacct cgatatcgtc gccgaccgcg tcgacgatcg caggcagggc ccggatggac
     1621 gcaggggtgc cgtccaggtt gttgccgccg tgattggaca ccgagatggc tgaaaccccg
     1681 acgtccacag cgcgtttcgc gtcgtcgacg cgcatcacgc ccttgagcat gaaccgacca
     1741 ccccacagct cgcgcagcca ggcgacgtct tcccacgtcg gcggcggggt gttcatccac
     1801 tccccgtacg ccgcgaagaa cggcgggccg ggctcgcccc gcggcgcctg gttgggtacc
     1861 cgcaggtcgg gcggacgcag ggtcttcgcc cactcccaca gccagacggg cttggtcagc
     1921 cccgtgggca tcatccgcac cgtcgtccgc aggttcatct gttcggggat ctttgggctg
     1981 ccccagtcgc ggccatggga gaagctccag tcggtggtca agatcagccc gacggccccg
     2041 gcctcgcgtg cgcgctctac gcgccgcgcg atggcctcgc ggtcaccgag ccagtagacc
     2101 tggaagaaga ccttttcgtt gacggcgatc acgtcttcga ccggcttgct cgcgaatgcg
     2161 gatagcccca tcgcggtgcc gcgggccgcc gccgcgcgcg cgaccgctac ttcgccgtcg
     2221 gggtgaaccg cctgcacgcc cgtcggcgag atcacgaccg gcagcgaaat atcctgtcca
     2281 agaacggtcg tcgtcatgtc acgcttgtcg gtcgcgccga tcacgtgcgg ggcgaagccc
     2341 agttcactga acgcgtcgat gttgtcggcg accgtcacgc cccgctcgct ggccgccagc
     2401 agcgctgcat acaccggctt cggcaggcgc cgcttggcct tctcctgggc gaacgccacc
     2461 gtctcaaacc attgggccat tactacaccg ggctttcgtt gcagagtcgg gtcggcggcc
     2521 gggacgtcag cgtcagcgca accggaccgc ggcgggaatg gtcaacgctt ggccggggca
     2581 cgtgccgctc acgggccagc gcaggcgcgc caaacccttg gacgcattcg ggatcgggcc
     2641 cgtcgatcgg cagaccggtg aagaacttcg ccgccatgca gccaccgcgg cagctgtcgt
     2701 agtgtccgca gctgctgcag gcgccggccg actgcggctc acgaagttca cgaaacagcg
     2761 gggcgttctt ccatacattg tcaaagccgc cgtcactcaa cacgtttccg gccaggaagc
     2821 ggtcatggat cgcaaacggg caggcgtata cgtcgccgac cggatcgatc aggcatacca
     2881 cccgaccggc gccacagaga ttgaggccgg ccaacgctcc cggctggccg agcggcgaca
     2941 ggtggaagaa cgaatcgccg gtgagcacgc ggtcgccccg gtcgacgagc cagttgtaca
     3001 gctgcacttg ctgttcggcg gtcggatgaa gttcgtccca cacgtcggcg ccccggccgg
     3061 acggccgcag tcgggtgatg cgtaacgtgg ccccgtaacg cgttgcaagc ccagcgaatt
     3121 cgtcgagctg gtcgacgttg tggcgggtca ccaccaccga gatcttggcg tccgtgaatc
     3181 ccgcggctgc gaggtggtcc aatgcccgaa ccgccatgtc gtaggagccg gcgccgcgca
     3241 ccgcgtcgtt gacgtccgcg gtggcaccgt cgagggagat ctgcacgtcg acgtagtcgc
     3301 tggcggcgag ccgctcggct ctcgcaggtg tgatgcgcac gccgttggtg gagaacttca
     3361 cgccgacgtg gtgggtggtg gcgtagtcga cgagctccca aaagtcaggg cgcacagtcg
     3421 gttcgccgcc accgatgttg acgtagaaca cctgcattcg ttcgagctcg tcgatcacgt
     3481 ccatgcactg gcgcgtggtc agctcacggg gatcacgcct gcccgacgac gacaggcagt
     3541 gcacgcatgc gaggttgcag gcgtaggtct gctcccacgt cagacagatc ggcgcgtcga
     3601 ggccacgctc gaactgctcc accaatcgag gtactgcggt caccctcgct cctgacccac
     3661 caacatgtcg gaggtggcga ggacctccag cgcgtgaaga tacggccgct caccgccggc
     3721 ggtgaccccg gccgcccggc acgcggactg tgcgtcggga tactcgccaa gtgattgcac
     3781 gatcgccagg atcgtgcggt ccttcaagaa cgacagcttg cgggtaccga agtggtacag
     3841 cagcgcgccg aacggctccg ggcgcaccgc cacttggggg tgcagccgcc agccgcgcgt
     3901 ggcgtcgaac acctcagtag acgccgcaca tgccgtcgat cgacacctct tcgacgaggg
     3961 tctcggtgac gagttcttct gtgcctgatg tcgccgacgc tgcggctccg gcggcttgtg
     4021 cttggctgtt cggttccatc gggctgcctt cctgctggtt gggtctcgac agattgcagt
     4081 tgactcgaca gaatatggca tcgagtgccg aaatggaagg gtggggtgtg gtgtggtgca
     4141 acctcgggta ggccggcgcc ggtcgacctc acgccaccag atcaccgacg tggcgatcgc
     4201 gctgttcgcc ggccgcgggt tcggcgaggt cagcgtcgac gacgtcgcag aggcggccgg
     4261 aatcgcccgc cggaccctgt ttcgctacta cccgtcgaag aacgcgatcc cgtggggcga
     4321 tttcgacgcc cacttgcagc acctgtggcg actgctggcg gcagtcgatc ccgcggtgcc
     4381 gatcgagcag gcccttcgcg atgcgttgtc ggcgttcaac acgtttgacg agtccgagac
     4441 ctcgcggcat cggcttcgga tgcaggtgat tctgcggacg acggaacttc aggcgtactc
     4501 gatgaccatg tacgccggct ggcgcgaagt catcgccgag ttcgtcgcgg cgcgctgcgg
     4561 gacgaagccc gacgatctgg tgccgcagac ggtcgcatgg gcgctgctgg ccgtctcgct
     4621 gtctgcctac gagcactggc tggccgacga gtcggtgtcg ttgacgcagg ctctggccga
     4681 cgcgttcgac gtcatccgcc ccggccgcga gctgctcacc tagttggcgc gagcgtgcgc
     4741 gtttgtaccc caacacaccg gtgtttggcc tacaaacgtg cacgctcacg cgaaaaaagt
     4801 cgggtgcggg gcgtcggagt tcggcgccca acggcgacga taaagatgtg agcgccgaac
     4861 ctcgtccgcc cgacgctccg cgtgcgctgc tcgcactgta cgaccaggcg ctgcccgcgg
     4921 tgtacggata cttcgcgcgc cgctgcggtg atcgcagcac cgccgaggac ctgacctcgg
     4981 aaacctttct caccgcgatg gacagcgctc gtaaggacca cccgcccgac gtcagcgtgc
     5041 cgtggctcat cggagtcgcc agacacaaac tggcagacca ctttcggcgc cggcccgacc
     5101 gtcgcgccgt gcccgtcgcc gaagtacccg aggatccgga tgacatctgg gacgccgaac
     5161 tggaccgcat cgtcgccgac agcacgctgc ggcggttgtc ggagccgcac cgcgcggtgc
     5221 tgacgctgcg ctacatggac gactgctcgg tgaacgagtg cgctgagctc atcggccgca
     5281 cggtccacgc taccgaggcg ctgctcgtgc gagcccgccg agcatttcgg caccactacg
     5341 aggaaggagg tgcgcgatga cacacgaccc gctaaatgtg ctacgcgccg acgaccttcc
     5401 ggtacaaccc gatcccgagt tcgcggcccg gctgcgggcc cggctcgaat ccgccctgtc
     5461 cctacccgca ggaaccgaag gagttgagat gagcggcacc gcccaggcgc tcgccgacct
     5521 gaccgaagca gcagcaccgc cacgcccggc ggccctgccc tacctggccg tggccgacgc
     5581 gcgcgctgcc atcgcgtggt acgccgacac gctgggcgcc gtactggtcg gcgagccgat
     5641 cgtgatggac gacgggcgga tcggccacgc cgagttggca ctggcaggag gtgttctcta
     5701 tctggctgac gagtaccccg agtttgggct gaaagcacct gcaccgcagt ctgtttcagt
     5761 gagcttgatg cttcacgtcg ccgacaccga tgcggcgctg cgccaggccc gtgaccacgg
     5821 ggcggtagtg cagcgggagg tctatgaagg ccacggaagc cgcaatgcca cgatcgtcga
     5881 cccgttcggc catcgctgga tgctcagcgg gcctgtcagc gcaccggtcg aggtgatccg
     5941 gcacggcgac ctgggttacg tctcggtgtg gacgcccgac gccgaccggg ccgcggcctt
     6001 ctacggccac gtgctgggct ggacgtacga cccgtcgaca catcaggtca ccaacacaca
     6061 gcagccggtc ggcctttttt ccgtcgctac gcatccgacg atgttctgct gctacgcggt
     6121 gtcggacctg caggccgccg cccgagcgat cgtcgccgcc ggcggccgag tcggcgaaga
     6181 gcgcgaattc ggcgtcggca ccgtgctcga tgcgacggac ccgacgggca ctgcgttcgc
     6241 ggtctaccgg gccgccgacg atgcgcgccg tctgccgctc aacggtgccg gccccggcga
     6301 attgtcctac gtcacttatg aagtcgataa ctcagcggcg ttccgtgagt tctatgggca
     6361 ggtcctgggg tggacgttcg agccaggcag cattcaggac ggctggcaga tcacgggcac
     6421 gcacccgatg gccggagttg ccggcggcag cgccgagccc acgacggtgc cgatgtggac
     6481 ggtggccgac atcgacgacg ccgtcgctcg ggtgcgcgag gccggcggca ccgttatgca
     6541 ggagccaaat aggcaaccgt acggtgtgat ggccgaatgc cgcgacgacc aaggcagccg
     6601 cttctacctc ggcgagttct cggctgcgcc gaaaccgaat tccaggtcgt gatcccggcc
     6661 cgaccacagc cctggtttca ctttcggcga cgccgacaag tcagttcaaa acctcgctga
     6721 tcggtgcgcc ggcggcgatc ttggtgcggg ttttcatcac cgctccgggc atgccgccac
     6781 cgactacgcc ggccaccacg ccgtcgcgct cgtagtacgc aaggaacttg cggccatcgt
     6841 cgttgaccag gtgcacgatg tcgtcagctt ccggctcgcc gaggcactgg atcttgatgt
     6901 cgtattggtc actccaaaag tacggcacga caaccagatt gtctggcaac tcttggccca
     6961 gcaacgacgg caccatgact cgtgcttgct cggcgacgtt gctccaatgc tcaacgcgcg
     7021 cttgatgtcc cgtcgcatcc tgccacgatg cgacgtcgcc caacgcccag atgtgtggtg
     7081 cactggtctt tccgacgtga tcgcacacca caccgttgtc gaccttcacg ccgctgtcct
     7141 cgagccagtc ggtggccggc cgagacccga tgccgacaac caccaggtcg gtatccaact
     7201 cggacccgtc ggtgagcacc accttctcca cccggcctga accggcaacc gacgagacgc
     7261 ccacaccagt gcgcacgtcg accccctcgg ctcggtgcag ccgcgccacc aacgcgccga
     7321 cctgctcacc cagcaccgac gccaacggct cgggctgggg ttcgaccagc acgacggcga
     7381 cgccgagccc gcgcaggctc gccgccacct cgcagccgat gaaccccgcg ccgacaacca
     7441 ccgcgcgccg tgccgcgccg gcctcctcgc gaagcgccat gctctcgtcg aaggagcgca
     7501 agacgtggat accccgcaag tctgggaatg acggaatgcg ccgcggcaca aggccagtcg
     7561 cgatcactgc ctcgtcatac tccagctgcg tcccgttttc cagcgtcacg gtctggttgg
     7621 cggtgtcgag cttgcgcacg gccgatccga gcagcagcgt gatgttgttc tcgtcgtaga
     7681 actcccgcgg attgagcgcc gtgtcgtcca tctcgctgcg tagcacctgc ttggacagcg
     7741 gcggccggtc gtacggcagg tgcacctcgt cgctgacgat cgtgatcgac ccgtcgaacc
     7801 cggatcgccg aagctgctcg gcggtgcgcg ccgctgccag tcccccgccg acgatgacta
     7861 tccgcttgct cattaccgtt tcatacacca tccggcactc ctggtttagg gcctcgtcct
     7921 gtcgatgaga ttcgacaaca cgacgatgct ctcgctgcgc tcgatgtctg cgctggatcg
     7981 gatgcgctcg agggcctcct cgaggtgctg catgtcgcgg gcaagcacat gcaggatcgc
     8041 gtcggaggtg ccggtgacgg tggccgcgct gacgacctcg ggaatgtcca cccacgccgc
     8101 gcggagacgc tccggtgcga tcgtgccctg acagaacacc tgcacgtacg cctcggtgtt
     8161 ccaccccagc gcgttgcggt cgaccacagt ggtaaatcct ttgatcacac cgcggtccag
     8221 catccggtcc actcgccgct tgaccgcggg cgccgacaaa ctcaccttct cgccgatctc
     8281 ggcgaaggtg gctctcgcgt gctcggccag ctcggccaag atgcgctcgt cgatgtcatc
     8341 caggcggtcc acgtagcgcc ctccagcgtc cacgcaacaa atcgaatact tccaggcatt
     8401 cgcgcaacag aatatcgcaa cacaagcaac atatggtgat tgattgcgcg aactgtccga
     8461 aatatcgtgg tttcgatgac ggagtacctt ttcgccgccg atgacgcctg tcgtgacgca
     8521 ccgtcgcgac tccatgcccg cacgccgcgt gcccgccgct acgtgatgac gccgccggag
     8581 ttcttcgctg tcgagtacgc gatcaatccg tggatggacg tggccacgcc ggtcgaccgg
     8641 cagatcgcgg ctcggcaatg ggagtcgctc tacgagacct atctgcgcct tggccacctc
     8701 gtcgagctag tccggcccga agccgggctg cccgacatgg tgtacgcggc c
//
LOCUS       DASWBF010000076         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_729384,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000076 DASWBF010000000
VERSION     DASWBF010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729384"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF874_02225"
     CDS             complement(<1..272)
                     /locus_tag="VF874_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX9174941.1"
                     /translation="MPEGDTVWHTAVKLRDGLAGKTLTRCDVRVPRFATVDLTGHTVD
                     EVLSRGKHLFIRVGPASIHSHLKMDGSWRVMTKNRGVRPSDHRVRI"
     gene            complement(279..>2092)
                     /locus_tag="VF874_02230"
     CDS             complement(279..>2092)
                     /locus_tag="VF874_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558678.1"
                     /note="similar to the DNA and RNA helicase superfamily II
                     and eukaryotic DEAD family of helicases; longest known
                     protein in E. coli; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX9174942.1"
                     /translation="LLGPLTHDEVAERSAAVDVGGWLEGLRASRRAVTVSYAGRTWWT
                     TVEDIGRLRDGVGIAVPPGVPVSFTEPVADPLAELIGRYARTRGPFTTADVAARFGLG
                     LRVGADVLGRLASDGKLVRGEFTTAATDSEQWCDAEVLRMLRRRSLAALRAQVEPVST
                     AAYGRFLPGWQQVGAKSLSGVDGVAGVVEQLAGVPVPASALEPLVLGQRVRDYQAAML
                     DELLASGEVLWSGAGAISGSDGWIALHMADTAPLSLPAPANIEFTDRHRLILDRLGRG
                     GGFFFRQLADDRGREEHKEHRGREEQKEHRDGEQLEAALWELVWAGWVTGDTFAPVRG
                     LLAGSHRRGRSAPAHRQRRRAPRLSHYSVAHAVARAADPTVAGRWSALPSPEPDSTVR
                     AHFSAELLLGRYGVLTKGSVTAEGVPGGFGTLYKVLAALEDAGRCQRGYFVESLGGAQ
                     FAVASTVDRLRSHQDSLDPDRPAYQAVALAAADPANPYGSALPWPTLASDDTTHRPGR
                     KAGALVVLVDGELVWFLERGGRSLLNFSSDPEARRAAAGALAELVSDGRVPTVLVERV
                     DGVPVLEPAQSGDRAEVAAALTDAGFARTPRGLRLRR"
BASE COUNT          314 a          773 c          722 g          283 t
ORIGIN      
        1 atgattcgca cccgatggtc gctgggccgc acgccgcgat tcttggtcat gacccgccaa
       61 ctgccgtcca tcttcaagtg cgagtggatg ctggccgggc cgacccggat gaacaaatgc
      121 ttgccgcggc tgagcacctc atcgacggtg tggccggtca aatcgactgt ggcaaaacgg
      181 ggcacccgca cgtcgcagcg ggtaagcgtc ttacccgcca gcccgtcgcg cagcttcacg
      241 gcggtgtgcc agacggtgtc gccctcgggc atgcgcgcct accgccgcaa ccgcaacccg
      301 cgcggcgtgc gcgcgaagcc ggcatcggtc agcgcggccg cgacttccgc ccggtcaccg
      361 gattgcgccg gctcgagcac cggcacgccg tcgactcgtt cgacgaggac cgttggaacc
      421 cgcccgtcgc tgaccaattc ggccaacgca ccggcagccg cgcggcgcgc ctccgggtcg
      481 gaggagaagt tcagcagtga acgaccgccg cgttcgagga accacaccag ctcgccgtcg
      541 acgagaacga cgagcgcgcc cgccttgcgg cccggccggt gagtggtgtc gtcagacgcc
      601 aacgtgggcc acggcagcgc tgagccgtac ggattcgccg ggtcggccgc ggccaacgcc
      661 acggcctggt atgccgggcg gtcggggtcg aggctgtcct gatgtgaccg cagccggtcg
      721 acggtggacg cgaccgcaaa ctgcgccccg ccgagggatt cgacgaaata gccgcgctgg
      781 cagcgaccgg cgtcctccaa cgcggccagc accttgtaca gcgtgccgaa cccacccggc
      841 acaccctctg cggtgacgct gcccttggtc agcacgccgt agcggccgag cagcagctcc
      901 gccgaaaagt gtgctctgac agtcgaatcc ggttctgggg acggcagcgc tgaccatctg
      961 ccggcgacgg tggggtcggc agcacgggcc acagcgtgcg cgacgctgta gtggctgagg
     1021 cgcggcgcgc gccgacgttg ccgatgtgcg ggcgccgagc ggccgcggcg gtgtgagccg
     1081 gccagcaggc cccgcaccgg cgcgaacgtg tcgccggtca cccagcctgc ccagacgagt
     1141 tcccacagcg ccgcttccag ctgttcgccg tcacggtgct ctttctgctc ctcgcgtccg
     1201 cggtgctctt tgtgctcctc gcgtccgcgg tcgtcggcca gctgccggaa gaagaacccg
     1261 ccgccgcgac cgagcctgtc gaggatcagc cggtggcgat cggtgaactc gatattcgcc
     1321 ggcgcgggca acgacagcgg cgcggtgtcg gccatgtgca gcgcgatcca gccgtcgctg
     1381 ccggagatcg cgccggcgcc ggaccagaga acctctccgg acgcgaggag ctcgtcgagc
     1441 atcgcggctt ggtagtcacg gacccgctgc ccgaggacca gcggctcgag ggccgacgcc
     1501 ggcaccggca cgccagcgag ttgttcgacc accccggcca caccgtcgac gcctgacaag
     1561 ctcttcgcgc cgacctgctg ccaccctggc aggaatcggc cgtaggccgc ggtgctgacg
     1621 ggctccacct gtgcgcgcag cgcggccagc gaccgccgcc gcagcatccg caacacctcg
     1681 gcgtcgcacc actgctcgga atcggttgct gccgtggtga attcgccccg gaccaacttg
     1741 ccgtcggagg ccagtcggcc cagcacgtcg gcgccgaccc gcaggccaag cccgaacctc
     1801 gccgcgacgt cagcggtggt gaacgggccc cgggtgcgcg cgtagcgccc gatcagctcg
     1861 gccagcgggt cggccaccgg ctcggtgaag ctcaccggta cgccgggtgg caccgcgatg
     1921 cccacgccgt cacgcagccg gccgatgtct tcgacggtcg tccaccaggt ccgtccggca
     1981 taggacacgg tcactgcgcg gcgggacgcg cgcagcccct ccagccagcc gccgacgtca
     2041 actgcggcgc tgcgttcggc gacctcgtcg tgggtgagcg ggccgagcag cc
//
LOCUS       DASWBF010000077         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_532225,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000077 DASWBF010000000
VERSION     DASWBF010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532225"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..309)
                     /locus_tag="VF874_02235"
     CDS             complement(157..309)
                     /locus_tag="VF874_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174943.1"
                     /translation="MPDDPGDLGRARRLIAEHSDLLGIELAQDKSAVGRWRVKGRRGG
                     LLAGGS"
     gene            complement(630..836)
                     /locus_tag="VF874_02240"
     CDS             complement(630..836)
                     /locus_tag="VF874_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174944.1"
                     /translation="MDDLCDDTPEGAPSWLSRPKADTTQSSGAWPGRGTLDTTDRGKY
                     KVLRRQFLEQAMARGDLCLARLCP"
     gene            complement(1045..1184)
                     /locus_tag="VF874_02245"
                     /pseudo
     CDS             complement(1045..1184)
                     /locus_tag="VF874_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            1241..2122
                     /locus_tag="VF874_02250"
     CDS             1241..2122
                     /locus_tag="VF874_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174945.1"
                     /translation="MKRGPELARRFFNRFEPVHGLTYFAPEVRAALDGLGYRGFWMGY
                     FAARSAPLGKVPPEVVTAVFYNFAPERVAKALTGAWAVAGPEAALRARHESAVAALRR
                     YGVTDGVQTAARLAGKAAKVAGVDGRPLFAANRALPWPDEPVLALWHAVTLLREHRGD
                     GHVAVLTAEGISGRESNVLHAAAGKVPREYMMRARDYDDASWQQHERALAERGLLDDT
                     GALTAAGARLKEHIELTTDRLALSALEALDDDDVETLFQVLTPITRLVIAGGDIPATT
                     PMGLGRDDLDDGSARLT"
     gene            complement(2500..>2682)
                     /locus_tag="VF874_02255"
     CDS             complement(2500..>2682)
                     /locus_tag="VF874_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174946.1"
                     /translation="VCITHPDVAKAVAYVADNGGTILSQAAAFVPGWPWLLAYVADPC
                     PSLVWPPPRCLHSFRR"
BASE COUNT          470 a          907 c          877 g          428 t
ORIGIN      
        1 ccaccgcgct cgcaccccgg tgcagccgcg acaggagcga ggcacggaac tcagcgagca
       61 gccggcgccg ctaagcgacg ctgtcagcct cagcggcacc cttcaccggg tcatcaaccg
      121 ccagcaacga cgacgcgccg aaaccccttg tgctactcaa gatccgcccg ccagcaaacc
      181 gccgcggcgg cccttgacgc gccaccggcc caccgcggac ttatcctgcg ccagctcgat
      241 ccccaacaaa tcggaatgct cagcgatcag ccggcgtgct cgtccgagat cgccggggtc
      301 gtcgggcacg tcgtcggcgt gccggctatg cagaaaattc gccgcgccgc gtttgaggtc
      361 cttaccagag cgtcaatacc taaccttatg gagggggcgt gccggcccag aaggaaagaa
      421 cgatgtgtca actcaaacac ttagcggagt tagcggcggt ggctggtgct ggtggccccg
      481 cggcgatgat tgtccttgga gttgcgaccg caccgacgcc caagttccgc cgaaccgatc
      541 aaacgaacca atcgaatcga tcccggacga cccacgtccc gccgaaccga ccgaccaacg
      601 tcgacgctac gcctcatata gggcggccgc taagggcaaa gccgcgcaag gcacaggtca
      661 ccgcgcgcca tggcctgttc gaggaactgg cgccgcaaca ccttgtactt tcctcgatcg
      721 gtggtgtcga gcgtgccacg accaggccac gcgccggatg actgtgtggt gtcggccttg
      781 ggccgactca gccaggacgg agcgccttct ggagtgtcgt cgcacaggtc atccacggca
      841 cctagcgtaa atctgggtat tccggaatct gggtattccg gcctgtaatt acagaattgt
      901 tgccattacc ggcgttcttc ctagcgcgcc aatcatgctg cggtgcaacg tatttcgcgc
      961 gctcagcaac gtaggtgttg cggggggtac cgggaggccg gagacctctc atgcgactct
     1021 cgttcgccgg cgactttgat ggtcgccggc ggtcatcttc gcgatgttgg ccggcgggtg
     1081 gaagtcgtcg gcgtcggcga acggcacccc gagtcgctgc gccagcgcgg cacgaccgtc
     1141 gacttgcccg atccggacac gcccatcacg acaatcggcg acatggttgg cgcgtacccc
     1201 gtttctgcac gtgtatgcac gttgaggtta actctggaat gtgaaacgag gacccgaact
     1261 cgcacggcgc ttcttcaacc gcttcgagcc ggtccatgga ttgacgtact tcgcgcccga
     1321 ggtgcgcgct gcgctggacg gccttggcta tcgcggcttt tggatggggt atttcgccgc
     1381 acggtcggcg ccgctgggca aggttccgcc cgaagtcgtc actgcggtgt tctacaactt
     1441 cgcaccggaa cgggtcgcca aggcgctgac gggcgcgtgg gccgtcgccg gtcccgaggc
     1501 ggcgctgcgg gcacggcatg aatcggcggt ggccgcgctg cgccgctacg gcgtcaccga
     1561 cggcgtgcag actgcggccc gcctggccgg taaggcggcg aaagtcgcag gggtcgatgg
     1621 gcgtccactg ttcgcggcga accgggcgct gccctggccc gacgagccgg tgctcgcgct
     1681 gtggcatgcg gtcacgctgc tgcgggagca tcgcggcgac gggcatgtcg ccgtgctgac
     1741 cgcggagggc atctcgggcc gggaatccaa tgtgctgcac gccgccgcag gcaaggtgcc
     1801 gcgcgagtac atgatgcgcg cccgcgacta cgacgacgcg tcatggcagc agcacgaacg
     1861 agccctggcc gaacgtgggc tgctcgacga caccggcgcg ttgacggcgg cgggcgcaag
     1921 gctcaaggag cacatcgagt tgaccaccga ccggctggcg ctgtccgcgc tcgaagcgct
     1981 cgacgacgac gacgtcgaaa cgctgtttca ggtgctcaca cccatcacgc ggctcgtcat
     2041 cgcgggcggg gacatcccgg cgacgacgcc gatgggactg ggccgcgacg atctcgacga
     2101 cggcagcgcg cggttgacct gacctttact tcgtcgggtc gtggccagtt catcagcgaa
     2161 taccgccatg ccgtgtcccc caccgcgccc ctgccttacc ccacctcacc aaaccgcacc
     2221 gagctgcaac ccattcactg taacggcaaa agcagccgca cgctggcact cctaccgccc
     2281 aggtgccacc gccaggcggg gcgctggtcg gccgtgggcc gacgctgacc aagcatgttt
     2341 cgactgaaac aagctgctag cggcggcctc acacaaggtc ctcgtcgagc cgggtgacga
     2401 ggttgtcccg tgccgcggcc gggccgccgg atcagctccg gccgaatttg gagcttcgcc
     2461 gcggccatgc cagcgccctt tccttcgacg tcgaggcgct caacgccgga agctgtgcag
     2521 gcagcggggc ggcggccaca ccaaagaagg acacggatct gcaacataag ccaagagcca
     2581 cggccatccc ggcacgaagg cggccgcctg cgacaggatt gttccaccgt tgtcggcgac
     2641 gtacgcgacg gccttggcga cgtcaggatg ggtgatgcat ac
//
LOCUS       DASWBF010000078         9853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_267239,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000078 DASWBF010000000
VERSION     DASWBF010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267239"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VF874_02260"
     CDS             <1..1516
                     /locus_tag="VF874_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX9174947.1"
                     /translation="SDAFTLHDTYGFPIDLTLEMAAEAGLAVDENGFRRLMAEQRERA
                     KADAAARKHAHADLSAYRELVDAGPTEFTGFDELASEARILGIFVDGRRVPVVAHNRD
                     RQAAERVELVLDRTPLYAESGGQIADEGTITGTGAGATAKAAVTDVQKIAKTLWTHRV
                     NVESGEFVEGDTVVAAVDPRWRHGATQGHSGTHLVHAALRQVLGPNAVQAGSLNRPGY
                     LRFDFNWQGGLSEDQRTQIEEVTNEAVEADFKVHTFTTELDKAKAMGAMALFGESYPD
                     EVRVVEIGGPFSVELCGGTHVHNSAQVGPVTILGESSIASGVRRVEAYVGLDAFHHLA
                     KERALLAGLASSLKVPSEEVPARVANLVERLKVAEKELERTRQASARATAANAAAGAE
                     QVGRVRVVAQRMSGETAGDLRNLVGDIRGRLGSEPAVVALIAEGEGGTVPYVVAANPA
                     AVDLGLRANELVRQLNQAVDGRGGGKAELAQGSGKNPAGIDGALSALRAEIAGS"
     gene            1602..2078
                     /gene="ruvX"
                     /locus_tag="VF874_02265"
     CDS             1602..2078
                     /gene="ruvX"
                     /locus_tag="VF874_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX9174948.1"
                     /translation="MPDRPGSDDPGRGRRLGIDVGSVRIGVAASDPDGILATPVETVR
                     RDRSGKHLRRLAALIDDLDVVEVVVGLPRTLADRAGPAAQDAIALADELAEHVPDVPV
                     RLADERLTTVTAQRSLREAGVRAKQQRSMIDQAAAVQILQGWLDQRRAPHGEALGG"
     gene            2071..3303
                     /locus_tag="VF874_02270"
     CDS             2071..3303
                     /locus_tag="VF874_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX9174949.1"
                     /translation="MADGRARERAEPVAVNAPRRKSRADRIRQERIRRRRNNTRMVIL
                     ALLVVVVVAAVFLGSRLWHSWFNDYSGDGKSDIVIQVHSGDSTTAIGQTLVDNKVVAS
                     VRGFVDAAHGNSAMSSIQPGYYQMRTEIPAADAVRRLTDPNNRVGKLVIPEGRQLDDV
                     QDVKTNAVTEGILTLISRASCVQLDGNRRCVPVTELRHVAGTDAPSALNVPSWAVTPV
                     QAMGDDPRRLEGLIAPGAWDINPSWTPQQILATLVGESATRYAELGLGTSPDGTTMSA
                     YQTLIVASLVQRESKPADFAKVARVIYNRLLARQRLEFDSTVNYPLDRQEVATTDADR
                     GRNTPWNTYFNEGLPKTPICSPGDQALAAAERPEPGDWLYFVTVDMQGTTLFTRDYQQ
                     HLANIELAKRNGVLDSAR"
     gene            3323..4135
                     /locus_tag="VF874_02275"
     CDS             3323..4135
                     /locus_tag="VF874_02275"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX9174950.1"
                     /translation="MSTVKAAVLGSPIAHSKSPQVHLAAYRALGLLDWTYERIECTAE
                     QLPTVVSGFGPEWVGVSVTMPGKFAALRFADEHTQRAEMVGSANTLLHTERGWRADNT
                     DVDGVIGALRSVDGYVDDNLVVLGSGGTARAAVVAAGGLHARGVTVVARDPARAEPLL
                     TLAAATGLEGRWRAFADPGLADEVAGAGAVVSTIPADAAAAHLAVLRRAPVLLDAIYD
                     PWPTPLAAAVTEAGGTVIDGLQMLLHQAFAQVEQFTGRPAPKEAMRAAVQRR"
     gene            4145..4573
                     /locus_tag="VF874_02280"
     CDS             4145..4573
                     /locus_tag="VF874_02280"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX9174951.1"
                     /translation="MGAGVCVVVLVWLAALSIYDVWQRRLPNWLTLPGAAAVLMAAAL
                     AGRGMPALAGASALGLLYLLVHAVAPAALGAGDVKLAVGAGALSGYFGVDVWLLAALA
                     APLLTAVWSLLELARRGARTVPHGPAMCAATAAAAALALL"
     gene            4677..5888
                     /gene="aroC"
                     /locus_tag="VF874_02285"
     CDS             4677..5888
                     /gene="aroC"
                     /locus_tag="VF874_02285"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX9174952.1"
                     /translation="MLRWTTAGESHGRALVSMLEGMVAGVHVTSADIRTQLARRRLGY
                     GRGARMKFEADEVTVLAGVRHGVTLGGPIAVEIGNTEWPKWETVMATDPVDPAELADI
                     ARNAPLTRPRPGHADYAGMLKYGFDDARPVLERASARETAARVAAGTIAKRFLGQALG
                     AEVLSHVISIGASKPYDGPPPQSADLDTIDASPVRAYDEQAEKSMIDEIEAAKRDGDT
                     LGGVVEVVALNLPVGLGSFTSGDNRLDSQLAAAVMGIQAIKGVEIGDGFETARRRGSV
                     AHDEMYPGAAGPGGVLRSTNRAGGLEGGMTNGQPLRVRAAMKPISTVPRALATVDMAT
                     GDEAVAIHQRSDVCAVPAAGVVAEAMVALVLARAVLEKFGGDSITETKNNIASYQRAV
                     AARDHDARASG"
     gene            5893..6393
                     /locus_tag="VF874_02290"
                     /pseudo
     CDS             5893..6393
                     /locus_tag="VF874_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            6518..7615
                     /gene="aroB"
                     /locus_tag="VF874_02295"
     CDS             6518..7615
                     /gene="aroB"
                     /locus_tag="VF874_02295"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX9174953.1"
                     /translation="MPNGRNVTEPVTVDVEVNPPYPVIIGNGLLGELTRLLDGTHKVA
                     ILHQPVLAETAEQIRTNLSDNGIDAHRIEIPDAEAGKELRVVGFIWEVLGRIGIGRKD
                     AIVSLGGGAATDVAGFAAATWLRGVSVIHVPTTLLGMVDAAVGGKTGINTEAGKNLVG
                     AFHQPKAVLVDLETLKTLPRNEIAAGMAEVVKAGFIADPTILDIIERDPEAAVEPDGP
                     VLKELIRRAVAVKAEVVAADEKESQLREILNYGHTLGHAIERRERYQWRHGAAVSVGL
                     VFAAELGRLAGRLDDATADRHRNILAALGLPVSYDRDALPQLIDFMAGDKKTRAGVLR
                     FVVLDGLAKPGRLEGPDPSLLAAAYAELEKP"
     gene            7612..8049
                     /gene="aroQ"
                     /locus_tag="VF874_02300"
     CDS             7612..8049
                     /gene="aroQ"
                     /locus_tag="VF874_02300"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX9174954.1"
                     /translation="MTTVNVLNGPNLDRLGLREPDVYGSTTYLELVALVEKTAADLGL
                     KALVRQSDSEAELIDLVHRAADAAEPVILNAGGLTHTSVALRDACAELRAPLIEVHIS
                     NVFAREEFRHHSYISPVATGVIVGLGVRGYLLALRYLADALKS"
     gene            complement(8051..8743)
                     /locus_tag="VF874_02305"
     CDS             complement(8051..8743)
                     /locus_tag="VF874_02305"
                     /inference="COORDINATES: protein motif:HMM:NF037996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-4DMT family transporter"
                     /protein_id="HEX9174955.1"
                     /translation="MRSWLLRGLIFGFLQTVIRVVQTMMINTWETQAAMISVLLQVIF
                     AVLVLLWGFFDGRNDANAQPDPDRRADLAMTWLIAGLVAGVLSGALCTLISLFYKDMY
                     VMGLVQELTVFAAYTALLVFLMAMIGVAIGRFLVDRRYNKEGYPSRRREGQEGDNPDT
                     DVFAAVGGGRSEATQEARAQEAPVHEGRTQEAGFDEGRTEEIRYDEGRTQEAPLHEGR
                     TQEAPSRPEREQ"
     gene            8777..>9853
                     /locus_tag="VF874_02310"
     CDS             8777..>9853
                     /locus_tag="VF874_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX9174956.1"
                     /translation="MTYSQRRDNLCRRLAAAGLDAMLVSDLVNVRYLSGFTGSNAALL
                     VVADADAPATLATDGRYRTQAARQAPEVRLEIERACGSHLTRLAADGGAARLGFESHV
                     VTVDQYDVLARAAGDVELVRASGTVEALREVKDDGEKALLRLACEAADAALADLIERG
                     GLRPGRTERQVARDLEALMLDHGADGVSFETIVAAGANSAIPHHRPTDAELASGDFVK
                     IDFGALVAGYHSDMTRTFVLGQAAQWQHDIYELVAAAQRAGREALAPGKVCSEVDAAA
                     RTVIADAGYAENFGHGLGHGVGLEIHEAPGISASSAATLQVGAAVTVEPGVYLPDRGG
                     VRIEDTLIVGDKAPELLTRFPKDLA"
BASE COUNT         1611 a         3310 c         3503 g         1429 t
ORIGIN      
        1 ctcggatgcg ttcacgctgc acgacaccta cggattcccg atcgacctca cgctcgagat
       61 ggccgccgag gccgggctcg cggtcgacga gaacggcttc cgcaggctga tggccgagca
      121 gcgcgagcgc gcgaaggccg acgccgccgc gcgcaagcac gcccacgccg acctgtcggc
      181 gtatcgggag ttggtcgacg ccgggcccac ggagttcacc ggattcgacg aattagcctc
      241 agaggcacgg attctcggta tcttcgtcga cggcagacgg gtgccggtgg tggcgcacaa
      301 ccgggatagg caggcggccg aacgcgtcga gctggtgctc gaccgcacac cgctgtacgc
      361 agagtccggc ggccagatcg ccgacgaagg cacgatcacc ggtaccggtg ccggagccac
      421 agccaaggcg gcggtcaccg acgtgcagaa gatcgcgaaa acgctttgga cgcaccgggt
      481 caacgtcgag tcgggcgagt tcgtcgaggg tgacaccgtg gtcgcggcgg tcgatccgcg
      541 gtggaggcac ggcgccaccc aaggccattc gggcacccac ctggttcacg ccgcgctgcg
      601 acaagtgctg ggccccaatg ctgttcaggc gggttcgctg aaccggccgg ggtatctgcg
      661 gttcgacttc aactggcagg gcgggctgtc ggaagaccag cgcacccaga tcgaggaagt
      721 caccaacgag gcggtcgagg ccgacttcaa ggtgcacacg ttcaccaccg aactcgacaa
      781 ggccaaggcg atgggtgcga tggcgctgtt cggtgagagc taccccgacg aggtgcgcgt
      841 ggtcgagatc ggcggcccgt tctcggtgga gctgtgcggc ggcacgcacg tgcacaactc
      901 cgcccaggtc ggtccggtca ccatcctcgg cgaatcgtcg atcgcgtcgg gggttcgtcg
      961 cgtcgaggcc tacgtggggc tagatgcgtt ccaccacctg gcgaaggagc gcgcgttgct
     1021 ggccgggctg gcgtcgtcgt tgaaggtgcc gtccgaggag gtcccggcgc gggtcgccaa
     1081 cctggtcgag cggctcaagg tggccgagaa ggagctcgag cggacgcggc aggcgtccgc
     1141 gcgcgcgact gccgcgaatg ctgccgccgg agcggaacaa gtcggtaggg tccgagtcgt
     1201 ggcccagcgg atgtcggggg agacagcggg ggacctgcga aaccttgtcg gcgacatccg
     1261 cgggcggttg ggcagcgaac cggcggtggt agcgctgatc gccgagggag agggcggcac
     1321 ggtgccttac gtggtggctg caaacccggc cgcggtcgac ctcggtctgc gcgccaacga
     1381 gctggtgaga caactcaacc aggcggtcga cgggcgcggc ggcggaaagg ctgaactggc
     1441 acagggatcg ggcaagaatc cggccggaat cgacggagcg ctgtccgcgc tgcgagccga
     1501 gatcgccggg agctagctgc gtggttctcg gtgatgacgg agccgcaggg ccggcgacat
     1561 cagatgacgg agccgcaggg ccggcgacat gtggaggacg cctgccggac cgacctggca
     1621 gcgacgaccc cgggcggggt cgccggctgg gcatcgacgt cggcagtgtc cgcatcgggg
     1681 tggccgccag cgaccccgac ggcatcctgg ccacgccggt ggagacggtg cgccgcgacc
     1741 gatccggcaa gcacctgcgc cggctggccg cgctgatcga cgacctcgac gtggtagagg
     1801 tggtcgtcgg cctgccccga acgctggccg accgcgccgg cccggccgca caggacgcga
     1861 tcgcgctcgc cgacgagttg gcggaacacg tacctgacgt tccggtgcgg ctggccgacg
     1921 agcggctgac gaccgtgacc gcgcagcgat cgttgcgtga ggctggtgtg cgagcgaagc
     1981 aacagcgctc gatgatcgac caggccgcgg cagtgcagat tctgcagggc tggctcgatc
     2041 agcgtcgggc gccacacggt gaggccctcg gtggctgacg gccgggcgcg tgagcgggca
     2101 gaacccgtgg cggtcaacgc gccgcggcgc aagtcccgcg ccgatcggat ccgccaagaa
     2161 cgaattcgtc ggcgccgcaa caacactcgg atggtaatcc tcgcactgct ggtcgtcgtg
     2221 gtggtcgctg cggtgtttct cggctccagg ctctggcaca gctggttcaa cgactattcc
     2281 ggtgacggca agagcgacat cgtcatccag gtgcacagcg gcgactcgac caccgccatc
     2341 ggtcagacac tggtggacaa caaggtcgtc gcgtcggtcc ggggattcgt cgatgcggcg
     2401 cacggcaatt cggcgatgtc gtcgatccag ccgggctact accagatgcg caccgagatt
     2461 ccggctgccg acgcggtgcg gcggctgacc gacccgaaca accgggtcgg caagctggtg
     2521 attccggaag gtcgccaact cgacgacgtg caggacgtca agaccaacgc ggtcaccgaa
     2581 ggcatcctca cgctgatctc gcgcgcgtcg tgcgtgcagc tggacggcaa ccggcggtgt
     2641 gtgccggtga ccgagctgcg ccacgtcgcc ggcaccgatg ccccctcggc gctcaacgtg
     2701 ccgtcgtggg ccgtcacgcc ggtgcaggcg atgggcgatg atccccgaag gctggagggc
     2761 ctgatcgcgc ccggcgcgtg ggacatcaac ccgtcctgga caccgcagca gatcctggcg
     2821 accctggtcg gcgagagcgc cacccgatac gcggaactcg gcctgggtac ctcgccggac
     2881 ggaacgacga tgtcggcgta tcagacgctg atcgtcgcgt cgctggtgca gcgcgagtcc
     2941 aagcccgccg acttcgccaa agtggcccgg gtgatctaca accggctgct ggcgcgccag
     3001 aggctcgagt tcgactccac ggtcaactac ccgctggacc gccaggaagt cgccaccacc
     3061 gacgccgacc gtggccgcaa tacgccctgg aacacctact tcaacgaggg actgccgaag
     3121 accccgatct gttcgccggg cgatcaggcg ctggcggcag ccgagcgacc tgagccgggg
     3181 gactggctgt acttcgtcac cgtggacatg cagggcacca cgctgttcac ccgggactac
     3241 cagcagcatc tggccaacat cgaactggcc aagcgcaacg gtgtcctcga cagcgcacga
     3301 tgagcctctc gggcgaagac cgttgagcac agtgaaagcc gcagtactcg gttcaccgat
     3361 cgcgcattcg aagtcgccgc aagtgcatct cgccgcgtat cgcgcgctgg gtctgctgga
     3421 ttggacgtat gagcgcatcg agtgcacggc agagcaattg ccaactgtgg taagcggttt
     3481 cggccccgaa tgggtgggtg tgtcggtgac gatgccgggc aagttcgccg cgcttcggtt
     3541 cgccgacgag cacacccagc gcgcggagat ggtgggttcg gcgaacacac tgctgcacac
     3601 cgagcgcggg tggcgcgccg acaacacgga cgtcgacggc gtcatcggcg cgctccgcag
     3661 cgtcgacggc tacgtcgacg acaacctggt cgtcctcggc tcgggcggca ccgcacgcgc
     3721 ggcggtggtc gcggcgggcg gcctgcacgc ccgcggggtg accgtggtcg cgcgtgaccc
     3781 tgccagggcg gagccactgc tgacgctggc cgcggccaca ggcctggagg gtcggtggcg
     3841 cgcgttcgcc gaccccggct tggccgacga ggtcgccggg gccggtgcgg tcgtcagcac
     3901 gatcccggcc gacgccgccg cggcgcacct cgcggtgttg cgacgcgcgc ccgtgctgtt
     3961 ggacgcgatc tacgatccgt ggccgacgcc gctggccgcc gcggtcacgg aggcgggcgg
     4021 cacggtgatc gacgggctgc agatgttgct gcaccaggcg ttcgcacagg tcgagcagtt
     4081 caccggccgg cccgcgccga aagaagcgat gagagcggcg gtccagcggc gctaacctcc
     4141 gagaatgggg gcgggggtct gcgttgttgt actggtgtgg ctggcggcgc tgagcatcta
     4201 cgacgtgtgg cagcgccggc tgccgaactg gctgacgctg ccgggagcgg cggcggtgtt
     4261 aatggccgcg gcgcttgccg gacgagggat gcccgcgctt gccggggcgt cggcgctggg
     4321 cctgctgtac ctgctcgtgc atgccgtcgc gcctgccgcg ttgggcgccg gcgacgtgaa
     4381 gctggcggtc ggtgccggcg cgctcagcgg ctacttcggc gtggacgtgt ggctgctggc
     4441 cgcgctggcc gcgccgctgc tgaccgcggt gtggtcgcta ctggagctgg cccgtcgcgg
     4501 cgcccgcacc gtgccgcatg gcccggccat gtgcgcggcg acagcggcgg cggctgcact
     4561 cgccctgctt tagctcgccg aaacggtatt ccacgtggtc ggtactcgca cttctccgcg
     4621 ctggttaccg cctcggcgat taagcccagc agccctcgca tgggaagatg ggacgcgtgt
     4681 tgcgatggac gacggccggc gagtcgcacg gccgcgcgct ggtgagcatg ctcgaaggca
     4741 tggtcgccgg cgttcacgtc acgtccgccg acatccgcac ccaactggcc cgacgccgcc
     4801 tcggctacgg ccgcggcgcc cgaatgaagt tcgaggccga cgaggtcacc gtgctggccg
     4861 gtgtgcgcca cggcgtcacg ctcggcggcc cgatcgccgt cgagatcggc aacaccgagt
     4921 ggcccaagtg ggaaaccgtc atggccaccg atccggtcga ccccgccgag ctcgccgata
     4981 ttgcccgcaa cgcgccactc acacgacccc gtcccggcca cgccgactac gccggcatgc
     5041 tcaagtacgg cttcgacgac gcccgcccgg tactggagcg ggccagcgca cgggagaccg
     5101 cggcccgcgt cgcggcgggc acgatcgcaa agcgattcct gggccaggcg ctgggcgccg
     5161 aggttctcag tcacgtgatc tcgatcggcg cgtccaagcc gtacgacggc ccgccgccgc
     5221 agtcggccga cctcgacacg atcgacgcca gcccggtccg cgcatacgac gagcaggctg
     5281 agaagtcgat gatcgacgag atcgaggccg ccaagcgcga cggcgacacg ctcggcggcg
     5341 tcgtcgaggt cgtcgcactg aacctgccgg tcgggctcgg ctcgttcacc agcggcgaca
     5401 accgcctcga cagccagctc gcggccgcgg tcatgggcat ccaggcgatc aagggtgtcg
     5461 agatcggcga cggcttcgag acggcaagac gccggggcag cgtcgcgcac gacgagatgt
     5521 atcccggagc cgcggggccc ggcggggtgc tgcggtcgac gaaccgcgcc ggcgggctcg
     5581 aaggaggcat gaccaacggt cagccgctgc gggtccgcgc ggcaatgaag ccgatctcga
     5641 cggtcccgcg ggcgctcgcc accgtcgaca tggccaccgg cgacgaagcc gtcgccatcc
     5701 accagcgctc cgacgtctgc gcggtgcccg ccgccggtgt ggtggccgag gcgatggtcg
     5761 cgctggtgct cgcgcgcgcg gtgctggaga agttcggcgg cgactccatc accgagacca
     5821 agaacaacat cgcgtcatat cagcgagccg tcgccgctcg cgatcatgac gcccgggcgt
     5881 cggggtagtg cgatggcgcc gaaagccgtg ctggtcgggc tgcccgggtc cggcaagtcg
     5941 acgatcggac ggcgtctggc ccgggcgctg gacgtgccgt ttctcgacac cgactcggcg
     6001 atcgaggcta agacgggccg cacgatcgca gacattttcg ccaccgacgg cgaacaagaa
     6061 ttccgccgca tcgaggaaga ggttatacgc gcggcgctcg ccgagcacga cggagtgctc
     6121 gcgctcggcg gcggcgcgat caccagtccg ggcgtccgcg acgcgttggc cgggcacacc
     6181 gtgatctatc tggaaatcaa cgcggccgag ggggtgcgcc gcacgagcgg cagcaccgtg
     6241 cgcccgctgc tggccggccc ggacccggcc ggtaaatacc gtgcgctggt ggctgatcgg
     6301 gtgccgctct accgtcgtgc ggcgacgatt cgcgtcaaca ccaaccggcg caacccgggc
     6361 gccgtcgtgc gccaaatcgt cgggcgactg gaaaacccgc acgcgcccgc cccccgacgc
     6421 cgcaagcgac gcgccgcctg gcgccgctca cccatctcgc tgacgccgag cgaatccacg
     6481 caagcgccac ctagccccgc ggccgtggcc gcgcgcaatg ccgaacggac gcaacgtgac
     6541 tgagcccgtg accgtcgacg tcgaagtcaa cccgccgtat cccgtcatca tcggcaacgg
     6601 actgctcggc gaattgacgc ggctgcttga cggcacccac aaggtcgcga tcctgcacca
     6661 gcccgtgcta gccgagacag cggaacagat acgaacgaac ttgtccgaca acggcattga
     6721 cgcgcatcgc atcgagatcc ctgacgccga agcaggcaag gaactgcggg ttgtcggctt
     6781 catttgggaa gtgttgggcc gcatcggtat cggtcgcaaa gatgcgatcg taagcctcgg
     6841 cggcggtgcc gccaccgacg tcgccggatt cgccgccgcc acctggctgc gcggagtgtc
     6901 ggtcattcac gtcccgacca cgctgctcgg tatggtcgac gcggcggtcg gcggcaagac
     6961 ggggatcaac accgaggcgg gtaagaacct cgtcggcgcg ttccaccagc ccaaggccgt
     7021 gctcgtcgac ctcgagacgc tgaagacgtt gccgcgcaac gagatcgccg ccgggatggc
     7081 cgaggtggtg aaggcaggct tcatcgccga cccgacgatt ctggatatca tcgagcgtga
     7141 ccctgaggcc gccgtcgagc cggacggacc ggtcctcaaa gaactgatcc gccgcgccgt
     7201 ggcagtcaag gccgaagtcg tggcggctga cgagaaggaa tcgcagctgc gcgagatcct
     7261 gaactacggg cacacgctgg gccacgccat cgaacgccgc gaacgctatc agtggcggca
     7321 cggcgccgcc gtgtcggtcg gactggtgtt cgcggcggag ttgggccggc tggccggccg
     7381 gctcgacgac gcgaccgccg accggcaccg aaacatcctg gccgcgctgg gcctgccggt
     7441 cagctatgac cgggacgcac tgccgcagct gatcgatttc atggccggcg acaagaaaac
     7501 ccgcgccggc gtgctgcggt ttgtggtgct cgacgggcta gccaaaccgg gacggctgga
     7561 aggtccggac ccgtcgctct tggcggcggc ctacgcggag ttggagaagc cgtgacgaca
     7621 gtgaacgtgt tgaatggccc caacctcgac cggctgggac tgcgcgagcc ggacgtgtac
     7681 ggcagcacca cctatctcga gctcgtcgcg ctcgtcgaga agacggccgc ggacctcggg
     7741 ctgaaagccc ttgtgcggca gagcgacagc gaagccgagc tgatcgacct ggttcaccgg
     7801 gcggccgacg ctgccgagcc agtgatcctc aacgccggcg gtctgacgca cacctcggtc
     7861 gcgttgcgcg acgcatgtgc ggaactgcga gcaccgttga tcgaggtgca tatctcaaat
     7921 gtgttcgcgc gcgaggagtt ccggcatcac tcctacatca gccccgtggc gacgggcgtg
     7981 atcgtcggac tgggcgtgcg gggatatctg ctggccctgc gctacctcgc ggacgcgctg
     8041 aagtcctaag ttactgttcc cgctccgggc ggctcggtgc ttcctgcgtc cggccctcat
     8101 ggagcggcgc ttcctgcgtg cggccctcgt cgtaccggat ttcctccgtt cggccttcat
     8161 cgaagcccgc ttcttgcgtc cgcccttcgt gaaccggcgc ctcctgagcc cgcgcctcct
     8221 gcgtcgcctc ggaccggccg ccaccgaccg ccgcgaacac gtcggtgtcc gggttgtcgc
     8281 cctcctggcc ttcgcggcgc cgagacgggt agccctcttt gttgtagcgg cggtcgacca
     8341 ggaagcggcc gatcgcgacg ccgatcatcg ccatcaagaa caccaacagc gcggtgtagg
     8401 cagcgaacac cgtgagctcc tgcaccagcc ccatcacata catgtccttg tagaacagcg
     8461 agatgagcgt gcacagcgcg cctgaaagca caccggcgac aagaccggcg atcagccacg
     8521 tcatcgctaa atcggcgcgc cggtcagggt cgggttgcgc gttggcgtcg ttgcggccgt
     8581 caaagaaccc ccagagcagg accagcacgg cgaagatcac ctgcaataag acgctgatca
     8641 ttgctgcctg cgtctcccag gtattgatca tcatggtctg tacgacccga atgaccgttt
     8701 gcaggaaccc gaagatcaac ccgcgcagca accatgacct catggccggt cagcctagcg
     8761 ggtagcgtca agtgccgtga catattccca gcgccgcgac aacctgtgtc ggcggctagc
     8821 ggccgccgga ctggacgcga tgctggtatc agacctggtc aatgtccgtt atctgtcggg
     8881 gttcaccggc tccaacgcag cgctgctggt cgttgccgac gccgatgcgc cggccacgct
     8941 ggccaccgac ggccgctacc gcacccaggc tgcacggcag gcgcctgagg tgcggctcga
     9001 gatcgagcgg gcgtgcgggt cgcacctcac ccggctggcc gctgacggcg gggcggcgag
     9061 gttgggcttc gaaagccacg tcgtcacggt cgaccagtac gacgtgttgg cgcgagccgc
     9121 cggcgacgtg gagttggtgc gggcctccgg cacggtcgag gcgttgcggg aggtcaagga
     9181 cgacggggag aaggcgctgc tgcggctcgc gtgcgaggcc gccgacgcgg cgctggccga
     9241 cttgatcgag cggggcgggc tgcgcccggg acgcaccgaa cggcaggtgg cgcgcgacct
     9301 cgaggcgctg atgcttgacc acggcgccga cggggtgtcg ttcgagacga tcgtcgcggc
     9361 gggagcgaac tccgcgatcc cgcatcaccg gcccaccgac gccgaactgg cgtcgggcga
     9421 cttcgtgaag atcgacttcg gcgcgctggt cgccggctac cactccgaca tgaccaggac
     9481 gttcgtgctg ggccaggccg cgcaatggca gcatgacatc tatgagttgg tggccgccgc
     9541 gcagcgcgcc ggccgagagg cactcgcccc cggcaaggta tgcagcgagg tcgacgcggc
     9601 cgcgcgcacc gtgatcgccg acgccggcta tgccgagaat ttcgggcacg gactcggtca
     9661 cggtgtcggg ctggagatcc atgaagcgcc gggaatcagc gccagctccg ctgcaacact
     9721 gcaagtcggc gccgcggtga ccgtggagcc cggtgtctac cttcccgacc gcggcggtgt
     9781 ccgcatcgag gacacgttga tcgtgggcga caaggccccg gaactgctga cccggttccc
     9841 gaaggacctg gcg
//
LOCUS       DASWBF010000079         9402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_793673,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000079 DASWBF010000000
VERSION     DASWBF010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793673"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VF874_02315"
     CDS             complement(<1..1457)
                     /locus_tag="VF874_02315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX9174957.1"
                     /translation="MTAALNAARRAADLAELADGAAVDVVVIGGGITGTGVALDAAAR
                     GLRVVLVEKHDLAFGTSRWSSKLVHGGLRYLATGNVGIARRSAVERGILMSRNAPHLV
                     RAMPQLVPLLPSMSRGNRALVRTGFLAGDVLRRLAGTSASTLPRSRRVDAGRASELVP
                     AVRRDGLAGGLLAYDGQLVDDARLVVAVARTAAQHGARILAYVAASDVTGDSVRLTDQ
                     RTGESFDLSARAVINATGVWAGDIDTSIKLRPSRGTHLVFDAAAFGNPTAALTIPIPG
                     EINRFVFAMPEQLGRIYLGLTDEDAPGPIPDVPQPTSEEITFLLDTVNTGLGVALGQQ
                     DVIGAYAGLRPLVDAGEGRTADISRDHAIIESASGMLSIIGGKLTEYRHMAEDVLDRA
                     VESRGLAAGPCRTRNLPLVGAQGGAQSVGLPTSLVARYGAEAQNVIASATCERPVEPV
                     ADGIDVIRAEFEYAVSHEGALNVDDILDRRTRIGLV"
     gene            complement(1457..2032)
                     /locus_tag="VF874_02320"
     CDS             complement(1457..2032)
                     /locus_tag="VF874_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9174958.1"
                     /translation="MMSISNAGVDIGARILDAAASCVAAYGIDRVTLAEIARRAGVSR
                     PTVYRRWTDTRQILAALLTTRVIGVWRDVPSQGRGRAALVERIVAVADQLRRDELIMS
                     VLRSTPELAMTYITERLGTSQQVLIDAAAAEIEAAQRDGSVRAGDPRQLAAMVLLITL
                     STIQSAPIVRPILDDDALARELTHTLNGYLA"
     gene            2056..3633
                     /locus_tag="VF874_02325"
     CDS             2056..3633
                     /locus_tag="VF874_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9174959.1"
                     /translation="MKWNAWGDPAAAKPLSDGIRSLLKQALGVGEAEVPAPEIDEVRL
                     RPSALSTDDRDALAAIVGSEFVLDDDRARLLHAGGKSTLDLLRRKQIEQDAPDAVVVP
                     GDEEQIAAILRHCSEHGIAVVPFGGGTSVVGGLDPDRNGFGAVISLDLQRLDELHSLD
                     EVSCEAVLGAGVSGPDAERLLGERGFSLGHFPQSFEFVTIGGLAATRSSGQDSAGYGR
                     FNDMIRGIRAVTPAGVLDVGRAPESAAGPDLRHLLIGSEGVFGVITQVRLKVHPVPEC
                     TRYEAWSFPDFATGAAALRAVTQIGTGPTVIRLSDEAETGVNLATSESIGEARITGGC
                     LAVTVFEGTTEHAESRHAETRAVLEKNGGTSLGEAPARAWEHGRFSAPYLRDALLAVG
                     ALCETLETATTWSNIPAVKDAVTQALTSSLGESGTPALVLCHISHVYPTGASLYFTVV
                     AGQRGNPIEQWRAAKTAATDAMVRTGATITHHHAVGADHRPWMRDEVGDLGVEVLKAV
                     KAALDPAGILNPGKLIP"
     gene            3630..4541
                     /locus_tag="VF874_02330"
     CDS             3630..4541
                     /locus_tag="VF874_02330"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX9174960.1"
                     /translation="MKTEISRVTLLTNPASGHGNAPHAAERAVAQFQKRGVDVVEIVG
                     TDAEHARRLIDEAVAVGADALVIAGGDGVISLALQALARSDIPLGIIPAGTGNDHARE
                     YGLPRDPVAAADVVADGWVETVDLGHIHGQKTEKWFGTVMAAGFDSLVSDRTNRMRWP
                     HGRMRYNLAMIAELSKLRLLPFRLVLDGEREIVADLTLAAFGNTKSYGGGMLICPTAD
                     HADGLLDITMVHSASRTKLVRLFPTVFKGTHIHLDEVKTDRAKTVTVESPGINAYADG
                     EFAMPLPVEVSAVSGALKILRSADHPI"
     gene            complement(4529..4840)
                     /locus_tag="VF874_02335"
     CDS             complement(4529..4840)
                     /locus_tag="VF874_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9174961.1"
                     /translation="MITIRLLAAAALAAGALLTAQTSSASPAEDVLFAALSAAGVPIT
                     SRESAIAAGELACDSATSGIARDLVAEQVSDKTGLDLGQATTFVGIALAVYCPFQPQI
                     G"
     gene            complement(4982..5524)
                     /locus_tag="VF874_02340"
     CDS             complement(4982..5524)
                     /locus_tag="VF874_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HEX9174962.1"
                     /translation="MRASNQFADATTGVVYIHASPAAVCPHVEWALSSTLEPHGRRHS
                     TGASRPILSWTPQPAMPGQLRAVTNWVGPVRTGGRLAHALRSWPVLRFEVTEDPSPGV
                     DGQRFCHTPQLGLWNGSMSANGDIVVGEMRLRSLMADGADALFAELDSVLGTAWDDAL
                     EPYRDGGHGAEVSWLNRGVG"
     gene            complement(5630..6454)
                     /locus_tag="VF874_02345"
     CDS             complement(5630..6454)
                     /locus_tag="VF874_02345"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9174963.1"
                     /translation="MSALDAISDWPVDAAAAAVVGPSGVLAEFGDQSRQFALASVTKL
                     LVARAAHIAVEEGVVELDTAAGPLGSTVRHLLAHASGLSMRSDERINEPGERRVYSNY
                     GFQVLAEAVQDKSGIEFGRYLHEALFAPLSMANTRLDGGADAAGYGATSTVADLVRFA
                     GDLLRPVTVAGETHAEATSVQFPGLDGVLPGFGVQRPNGWGLGFEIKHGKSPHWTGSQ
                     NSPGTYGHFGQTGTFLWVDPAADLALVVLTDRDFDKWARERWPDLSDTVLAEFAAD"
     gene            complement(6451..7500)
                     /locus_tag="VF874_02350"
     CDS             complement(6451..7500)
                     /locus_tag="VF874_02350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9174964.1"
                     /translation="METTEEFAARVMSTIDSASLAILLSIGHQTGLFDAMAGLPSSTS
                     SQIADAAGLNERYVREWLGGMTVGQIVEYDPGAGTYTLPPERAALLTRRAGPDNLART
                     AQFIPMLGEVEQKVIGCFRTGGGLSYSEYPRFHAVMAEESGEVVDAALVDVILPLAAG
                     LPERLRAGADVADFGCGSGHAVNVMARAFPASRFTGFDFSDEGLAVGAEEAAGLGLAN
                     ATFDRHDLADMDVTEAYDVITAFDAIHDQAQPDRVLRNIYRALRPGGVFLMVDIKASS
                     QLEDNVGVPLATYLYTVSTMHCMSVSLAFGGAGLGTAWGRQLALSMLSDAGFKDVRVE
                     EIDTDPINYYYVANK"
     gene            7536..8621
                     /locus_tag="VF874_02355"
     CDS             7536..8621
                     /locus_tag="VF874_02355"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HEX9174965.1"
                     /translation="MTQTVRGVISRKKGEPVEVVDVVVPDPGPGEVVVDVAACGVCHT
                     DLTYREGGINDEYPFLLGHEAAATVESVGEGVTDVEPGDFVVLNWRAACGQCRACKRG
                     RPWYCFDTHNAAQKMTLTDGTELTPALGIGAFVDKTLVHEGQCTKVDPAADPAVVGLL
                     GCGVMAGLGAAVNTGNVSRDDTVAVIGCGGVGDAAIAGSALVGAKRIIAVDTDDAKLV
                     WAKDFGATDTINARTADVVEAIQELTDGFGVDVVIDAVGRPETWKQAFYARDLAGTVV
                     LVGVPTPDMRIDMPLVDFFSRGGSLKSSWYGDCLPERDFPTLTSLYLQGRLPLDKFVT
                     ERIGIEDVENAFHKMHGGEVLRSVVIL"
     gene            8618..9235
                     /locus_tag="VF874_02360"
     CDS             8618..9235
                     /locus_tag="VF874_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9174966.1"
                     /translation="MIDRVVTHGTFELDGGSWEVDNNIWLIGDDDDVVVVDAAHDAGP
                     IIDAAAGRNVVAVVCTHGHNDHVTVAPQLGEALDAPVLMHPADEVLWRMTHPDKQFRV
                     LEDGAVLTVGGIELRAIHTPGHSPGSTCLHAPELSAVFSGDTLFQGGPGATGRSYSDF
                     PTILASISEKLGKLPGDTVVYTGHGDTTTIGGELVNYDQWVARGH"
     gene            complement(9232..9393)
                     /locus_tag="VF874_02365"
     CDS             complement(9232..9393)
                     /locus_tag="VF874_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174967.1"
                     /translation="MHWVVALSAGSTTQNVLPFTDLKEPWGVAVDRDGAVYVTDIRTS
                     RVLKLAAAS"
BASE COUNT         1526 a         3180 c         3238 g         1458 t
ORIGIN      
        1 acaagcccga ttctcgtgcg ccgatcgagg atgtcgtcga cgttgagtgc cccctcgtgg
       61 ctgaccgcat actcgaattc ggctctgatg acgtcgattc cgtcggcgac gggttcgaca
      121 gggcgctcac aggtggccga cgcgatgacg ttctgcgcct ccgcgccgta gcgggcgact
      181 agcgaagttg gcagaccgac cgattgcgcg ccgccctgcg cgccgaccag tggcagattg
      241 cgggtgcggc acggaccggc ggcgaggccg cgtgattcga cggcacggtc gaggacgtct
      301 tcggccatgt gccggtactc ggtgagcttt ccgccgatga tgctcaacat ccctgaggcc
      361 gactcgatga tcgcgtggtc ccgcgagatg tcggcggtgc ggccttcccc ggcgtccacc
      421 agcggccgca gcccggcgta cgcgccgatc acatcctgct ggccgagcgc aacgcccagt
      481 cccgtgttga cggtgtccag caggaaggtg atctcctctg aagtcggttg cggcacatcc
      541 ggaatcggcc cgggcgcgtc ctcgtcggtc agcccgaggt agatcctgcc cagctgctcg
      601 ggcatcgcga acacgaagcg gttgatctcg ccgggaatcg gaatggtcaa cgccgccgtg
      661 ggattgccga atgccgcggc gtcgaacacc agatgcgttc cgcgactggg ccgcagcttg
      721 atcgacgtgt cgatgtcgcc ggcccacacg ccggttgcgt tgatcaccgc gcgggcactg
      781 aggtcgaacg attcgccggt gcgctggtcg gtcaggcgca cggaatctcc ggtgacgtcg
      841 gatgccgcga catacgccag gattcgggcg ccgtgctgcg ccgcggtgcg tgcgacggcc
      901 acgaccagcc gcgcgtcgtc gacgagttgt ccgtcgtagg ccagcagtcc gcccgcgagc
      961 ccgtcacgcc gaaccgccgg gaccagctca gaggcgcggc cggcgtcgac ccgccgtgat
     1021 cgcggcaatg tcgaggcgct ggtgccggcc agccgccgca gcacgtcgcc ggccaggaac
     1081 ccggtgcgca ccagcgcccg gttgccccgg ctcatcgacg gcagcagcgg taccagctgc
     1141 ggcatcgccc gcacgaggtg aggagcgttg cggctcatca ggattccgcg ctcgaccgcg
     1201 ctgcgccgcg cgatgccgac gttgccggtc gcgagatacc ggaggccgcc gtgaaccagc
     1261 ttgctgctcc acctgctggt gccgaacgcc aggtcgtgct tttccaccag caccacccgc
     1321 aggccgcggg cggcagcgtc gagcgcgaca ccggttccgg tgatgccacc gccgatcacg
     1381 acgacgtcga cggcggcccc gtcggccaac tcggcgaggt cggcggcgcg gcgggctgcg
     1441 ttgagcgcgg cggtcatcag gcgaggtatc cgttcagtgt gtgggtcagc tcgcgcgcga
     1501 gcgcgtcgtc gtcgaggatc ggccgcacaa tcggcgccga ctggatcgtc gacagcgtga
     1561 tcagcaggac catcgcggcg agttggcggg gatcgcccgc gcgtacgctg ccatcccgtt
     1621 gcgccgcttc gatttccgcg gcggccgcgt cgatgaggac ctgctggctg gtgccgaggc
     1681 gttcggtgat gtaggtcatc gcgagctccg gggtggagcg cagcacggac atgatcagct
     1741 cgtcgcggcg cagctggtcg gccaccgcga cgatccgctc gaccagcgct gccctgcccc
     1801 tcccctgcga cggcacgtcg cgccacactc cgatgacgcg cgtggtgagt agcgcggcga
     1861 gaatctgccg cgtgtcggtc cagcgccggt agacggtcgg cctgctcacc ccggcgcggc
     1921 gggcgatctc cgccagcgtg acgcgatcga tgccgtaggc ggcgacgcag cttgccgcag
     1981 cgtcgaggat gcgggccccg atgtccaccc cggcgttact gattgacatc atatgtaata
     2041 ctgtaacgca tgccgatgaa gtggaacgcc tggggcgacc cagccgccgc caagccgctg
     2101 tccgacggaa tccggtcact gctgaagcag gcgctcggcg tgggcgaagc cgaggtgccc
     2161 gcgcccgaga tcgacgaggt gcggctgcgg ccgtccgcgt tgagcactga cgaccgtgac
     2221 gcactggcgg cgatcgtcgg atcggagttc gtccttgacg acgaccgcgc gcggctcctg
     2281 cacgccggcg gcaagtcgac actggacttg ttgcggcgca agcagattga gcaggatgcg
     2341 cccgacgcgg tggtggtacc gggcgacgag gagcagatcg ccgcgatctt gcgccactgc
     2401 tccgagcacg gtattgccgt cgtcccgttc ggtggtggca ccagcgtggt cggcgggctc
     2461 gatccggatc gcaacggatt cggcgcggtg atctcgttgg acctgcagcg actcgacgag
     2521 ctgcactcgc tggacgaggt gtcgtgcgag gcggtgctcg gcgccggggt cagcgggccg
     2581 gacgccgagc ggctgctggg tgagcgcggg ttctcgctcg ggcacttccc gcagagcttc
     2641 gagttcgtga cgatcggcgg cctcgccgcc acccgctcgt cgggacagga ctcggccggg
     2701 tacggccggt tcaacgacat gatccgcggc atccgcgcgg tcacgcccgc cggggtgctc
     2761 gacgtcggcc gcgctcccga gtcggcggcc ggaccggatc tgcggcacct gctgatcggt
     2821 tccgagggcg tgttcggcgt gatcacccag gtgcggctga aggtgcatcc cgttcccgag
     2881 tgcacccgct atgaggcatg gtcgttcccc gacttcgcaa cgggcgcagc ggctttgcgt
     2941 gccgtcacac agatcggcac tgggccgacc gtcatccggt tgtcggacga ggccgagacc
     3001 ggcgtgaacc tggccaccag cgagagcatc ggcgaggcga gaatcaccgg cggctgcctg
     3061 gcggtcaccg tgttcgaggg caccaccgag cacgcggaaa gccggcacgc ggagacacgc
     3121 gcggtgctgg agaagaacgg cggcacgtca ctgggcgagg cgcccgcccg cgcgtgggag
     3181 cacggccggt tcagcgctcc gtacctgcgg gacgcgctgt tagcggtggg tgcgctctgc
     3241 gagaccctcg agacagccac cacatggtcc aacatcccgg cagtcaaaga tgcagtgacg
     3301 caagcgctta cgtcgtcgct cggcgaatcg ggcacgccgg ctctggtgct gtgtcatatt
     3361 tcgcacgtgt atcccaccgg ggcgtcgctg tacttcacgg tggtggcggg ccagcgtggg
     3421 aatccgatcg agcagtggcg ggcggcaaag acggccgcga cagacgcgat ggtgcgcacc
     3481 ggcgcgacga tcacgcatca ccacgcggtc ggcgccgacc accgcccgtg gatgcgcgac
     3541 gaagtcggcg acctcggcgt cgaagtcctc aaagccgtca aggccgcgct cgatccggcc
     3601 ggaatcctca accccggcaa gctgattccg tgaagaccga gataagccgt gtcacgctgc
     3661 tgacgaatcc cgcatcgggg catggcaacg cgccgcacgc cgccgaacgc gccgtcgcac
     3721 agttccaaaa gcgcggcgtc gacgtcgtcg agatcgtcgg cacagacgcg gagcacgcgc
     3781 gtcggctgat cgacgaggcc gtggcggttg gcgccgacgc gctggtgatc gccggcggcg
     3841 acggcgtcat ctcgctggcc ctgcaggcgt tggcgcgcag cgacattccg ctcgggatca
     3901 tcccggccgg caccggcaac gaccatgcgc gggagtacgg cttgcctcgg gatccagtgg
     3961 cggcggctga cgtcgtcgcc gacggatggg ttgagaccgt cgacctcggg cacatccatg
     4021 ggcagaagac cgagaagtgg ttcggcaccg tgatggccgc cgggttcgac tcgctggtga
     4081 gcgatcggac caaccggatg cgctggccgc acgggcggat gcgctacaac ctggcgatga
     4141 ttgcggaact gtcgaagctg cggctgctgc cgtttcgtct cgtgctcgac ggcgagcgcg
     4201 agatcgtcgc ggacctgacg ctggccgcgt tcggcaacac gaaaagctat ggcggcggaa
     4261 tgttgatctg ccccaccgcc gaccacgccg acggcctgct cgacatcacg atggtgcatt
     4321 cggcgtcgcg cacgaagctg gtgcggctat tcccaaccgt gttcaagggc acgcacattc
     4381 acctcgacga ggtgaagacc gaccgcgcca agaccgtcac cgtcgaaagc cccggcatca
     4441 acgcctatgc cgacggcgag ttcgcaatgc cgctgcccgt cgaggtgtcg gcggtcagcg
     4501 gcgcgctgaa gatattgcgg tcagccgatc acccgatctg aggctgaaac gggcagtaca
     4561 ccgcaagggc gatcccgacg aacgtggtcg cctggccgag gtccaatccg gtcttgtcgc
     4621 tgacctgttc agcgacgagg tcgcgggcga tgccgctggt ggctgagtcg caggctaact
     4681 cacctgccgc gatagcactc tcccgcgacg taatcggcac gcccgccgcg cttaatgcag
     4741 cgaacaagac atcctctgcc ggtgatgccg aggaagtctg ggcggtgagc aatgcgccgg
     4801 cagccagggc agcggcggcg agcagacgga tagtgatcat ggtggtttcg cctcctcttg
     4861 gagacaacca cagtctcaca gcggatcctg cgctatgcgg atatgcgcgc gaaacccgcg
     4921 tttcgcacaa aaagttcgag aagcggcgtg ccaaactccg gtttcgcaga cgccgaggaa
     4981 gctaccccac tccccggttg agccagctga cctcggcccc gtgaccgccg tcgcggtagg
     5041 gctccaacgc gtcgtcccac gccgtgccca gcacagagtc caactccgcg aacagcgcgt
     5101 cggcgccgtc ggccatcaaa gaacgcagcc gcatctcgcc gacgacgatg tcgccgttgg
     5161 cgctcattga gccgttccac aacccgagct gcggggtgtg gcagaaccgc tgcccgtcca
     5221 cgcccgggct cggatcctcg gtcacctcga atcgcagcac cggccacgac cgcagcgcgt
     5281 gcgccaggcg gccgcccgtg cggacggggc cgacccagtt cgtcaccgcg cgcagctggc
     5341 cgggcatcgc cggctgcggc gtccaagaca ggatcggccg cgatgctcca gttgaatgtc
     5401 gccgcccatg cggctccagc gtggacgaca acgcccactc cacatgcgga cacaccgccg
     5461 cgggtgaggc gtggatgtac accacgcccg tcgtcgcgtc ggcaaactgg ttcgacgcac
     5521 gcatcctcta ctccttcggc tccacgaggg acgtcttccc caacgacctg gcgtaaccga
     5581 cgagtctgtg tcgtgcgtgt ctattgtgcc ttgagtgacg cctgttgcgc tagtctgccg
     5641 cgaattcagc cagcaccgtg tccgacaagt ccggccaccg ctcccgtgcc cacttgtcga
     5701 aatcgcggtc ggtgagcacg acgagcgcca ggtcagccgc cggatcgacc cacaggaacg
     5761 tgccggtctg cccgaagtgg ccgtacgtgc ccggcgagtt ctgcgagccg gtccagtgcg
     5821 gcgacttgcc gtgtttgatc tcgaagccga gaccccagcc gttaggccgc tgcacgccga
     5881 accccggcag cacaccgtcc agcccaggga actgcacgct cgtcgcctcc gcgtgggtct
     5941 cgccggcgac cgtgaccgga cgcagcaggt caccggcgaa ccgaaccagg tcggcaaccg
     6001 tcgacgtggc gccgtatccc gccgcgtccg cgccgccgtc gagtcgggtg ttggccatag
     6061 acagcggcgc gaacaacgcc tcgtgaaggt agcggccgaa ctcgatgccg gacttgtcct
     6121 gcacggcttc ggcgagcact tggaagccgt agttcgagta gacccggcgt tcgcccggct
     6181 cgttgatccg ctcgtcggag cgcatcgaaa gccccgacgc gtgtgcgagc aggtgacgaa
     6241 ccgtcgaccc gagcggaccg gccgcagtgt cgagttccac caccccctct tccaccgcga
     6301 tgtgcgcggc acgggccacc aacagcttcg tcaccgacgc cagcgcgaac tggcgactct
     6361 ggtcaccgaa ctcggccagc acgcccgacg gaccgacgac ggccgccgcg gcggcatcca
     6421 ccggccagtc cgagatcgca tcgagggcgc tcacttgttc gcgacgtagt agtagttgat
     6481 cgggtcggtg tcgatctcct cgacccgcac gtctttgaag ccggcgtcgg aaagcatcga
     6541 cagcgcgagt tgacgccccc acgccgtgcc gagcccggcg ccgccgaacg ccagcgacac
     6601 gctcatgcag tgcatggtcg agacggtgta gaggtacgtc gccagcggca caccgacgtt
     6661 gtcttcgagt tggctcgagg ccttgatgtc gaccatcagg aacacgccgc cgggccgcag
     6721 cgcgcggtag atgttgcgca gcacgcgatc cggctgagcc tggtcgtgga tcgcgtcgaa
     6781 cgcggtgatc acgtcgtagg cctcggtgac gtccatgtcg gcgaggtcgt gccgatcgaa
     6841 cgtcgcgttc gccaggccca ggcccgcagc ctcctcggcc ccgaccgcca agccttcgtc
     6901 ggagaagtcg aagcccgtga accggctggc cgggaacgca cgtgccatca cgttgacggc
     6961 atgcccactg ccgcaaccga agtcggccac atcggcgccg gcccgaagtc gctccggcag
     7021 cccggcggcg agcggcagga tcacgtccac caacgcggcg tcgaccacct cgccgctctc
     7081 ctcggccatc acggcgtgga atcgcgggta ttcgctgtag gacagcccgc cgccggtgcg
     7141 gaagcaaccg atgaccttct gctcgacctc gcccagcatg gggatgaact gggcggtcct
     7201 ggccaggttg tcggggccgg cccgacgggt cagcagcgcg gcgcgctcag gcggcaacgt
     7261 gtacgtgccg gcgccggggt cgtattcgac gatttgcccg acggtcatgc cgcccaacca
     7321 ttcccgcaca tagcgttcgt tcagtccggc ggcgtcggcg atctgcgaac tcgtcgacga
     7381 cggcagcccg gccatcgcat cgaatagccc ggtttggtgt ccgatggaca gcaggatggc
     7441 caggcttgcg ctgtcgatcg tgctcatcac ccgggcagcg aattcctcgg tggtctccat
     7501 accgtgatta tctctcggcc agtaggttgg cgcccatgac tcaaacagtt cgcggcgtga
     7561 tttcccgcaa gaagggcgag ccggtcgagg tcgtcgacgt ggtggttccc gatcccggac
     7621 ccggcgaagt ggtcgtcgac gtcgccgcgt gcggggtgtg ccacaccgat ctgacatacc
     7681 gcgagggcgg catcaacgac gagtacccgt tcctgctcgg ccatgaggcc gctgccacgg
     7741 tggagtccgt cggcgagggg gttaccgacg tcgaacccgg cgacttcgtg gtcctgaact
     7801 ggcgggcggc gtgcgggcag tgccgggcct gtaagcgcgg ccggccgtgg tactgcttcg
     7861 acacgcataa cgccgcgcag aagatgacgc tgaccgacgg caccgagctg acgccggcgc
     7921 tgggcatcgg cgcgttcgtc gacaaaacac tggtgcacga ggggcagtgc acgaaggtcg
     7981 acccggcggc cgacccggcc gtggtggggc tgctcggctg cggcgtgatg gccgggctgg
     8041 gtgcggccgt caacaccggc aacgtcagcc gcgacgacac ggtcgcggtg atcggctgcg
     8101 gcggagtcgg tgatgcggcg atcgcggggt cggcgctcgt cggcgccaag cggatcatcg
     8161 ccgtcgacac cgacgacgca aagttggttt gggccaagga tttcggggcc acggacacca
     8221 tcaatgcgcg cacagccgat gtcgtcgagg cgatccagga gctcaccgac gggttcggcg
     8281 tcgatgtggt gatcgacgcg gtcgggcggc ccgaaacctg gaaacaggcg ttctacgcgc
     8341 gggacctggc cggcaccgtg gtgctggtcg gggtgccgac gccggacatg cgcatcgaca
     8401 tgccgctggt ggatttcttc tcccgcggcg ggtcgctgaa gtcgtcgtgg tacggcgatt
     8461 gcctgcccga gcgcgacttc cccacgctga caagccttta tctgcagggg cgcctgccgt
     8521 tggacaaatt cgtcaccgaa cgcatcggca tcgaggacgt cgagaacgcc ttccacaaaa
     8581 tgcacggcgg cgaggtgctg cggtcggtgg tgatcctgtg attgaccgcg tggtcacgca
     8641 cggcacgttc gaactcgacg gcggcagttg ggaagtcgac aacaacatct ggctgatcgg
     8701 tgatgacgac gacgtcgtcg tggtcgacgc cgcccatgac gcgggcccga tcatcgacgc
     8761 ggcggcgggc cgcaacgtgg tggcggtggt gtgcacgcac ggccacaacg accacgtgac
     8821 cgtcgcgccg caactcggcg aggcgctcga cgccccggtg ctgatgcatc ccgccgacga
     8881 ggtgctttgg cgaatgacgc acccggacaa gcagtttcgc gtcctggagg acggcgcggt
     8941 gctaaccgtc ggcggcatcg aactgcgggc gattcacaca ccgggccact cccccggctc
     9001 gacatgcctg cacgcaccgg aattgagtgc ggtgttcagc ggcgacaccc tgttccaggg
     9061 cgggccgggc gcgacaggcc ggtcctactc ggacttcccg acgatcctcg cgtcgatctc
     9121 ggaaaagcta ggcaagctgc ccggcgacac cgtcgtctac acgggccacg gcgacaccac
     9181 cacgatcggc ggcgagctgg tgaactacga ccagtgggta gcgcgaggcc actagcttgc
     9241 ggcagccagc ttcaggacgc gactggtgcg gatatcggtg acgtacacgg caccgtcgcg
     9301 gtccaccgcc acaccccatg gttctttcag gtcggtgaag ggcagaacat tctgggtcgt
     9361 ggagccggcc gacagggcca ccacccaatg cacctcgtag tc
//
LOCUS       DASWBF010000080        19304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_402703,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000080 DASWBF010000000
VERSION     DASWBF010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19304
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402703"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VF874_02370"
     CDS             <1..206
                     /locus_tag="VF874_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014348762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9174968.1"
                     /translation="VAVDVLRGAPFETEQDPIITRMWALQRSAMYRDMGVIGVNVVSW
                     PEDVTLDQSMRLVPDHLHGAGRR"
     gene            220..663
                     /locus_tag="VF874_02375"
     CDS             220..663
                     /locus_tag="VF874_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174969.1"
                     /translation="MQPAPLAFSTAFGLLMVAAAAIDADRAGAVAVAVAVGALLIGLR
                     YRAAATLSVLLVVFAIVLSDPPALYAALAGLSAAAYLVLRYAASLGVVTTTRSTVIGT
                     VGFTLVGIAATVVPVSVPWLPLVAPVAAVAVFLVAALPLMRGLSD"
     gene            complement(653..1504)
                     /locus_tag="VF874_02380"
     CDS             complement(653..1504)
                     /locus_tag="VF874_02380"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9174970.1"
                     /translation="MTSVGVIGLGLIGSALAHRLIETGDPPLVFDVNPEAVEAAASQG
                     AIPAGSSAGLAERCDIVLVCVQTDDQCVAAVCGESGALEGAKPGTVIAVLSTVAPSTI
                     ESLAATAGQKDVHLVDTPIAGRGMFSVEEGTMSVMVGDDGELVTRLEPTLRRFASKVV
                     PAGRVGSGAALKLAHNIVVYAGFAAMIEAVELARAAGARDGLVEEVAQASGALSSLSA
                     FVLPFYKHFRDDPHGADEDEKLRVAAALLEKDLADAVALAASHNVDVPVARLLSHFGD
                     AIFPVSR"
     gene            1574..3016
                     /locus_tag="VF874_02385"
     CDS             1574..3016
                     /locus_tag="VF874_02385"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9174971.1"
                     /translation="MTTVAIIGAGMSGLCMAIKLQEAGIESYTIFEQASDVGGTWRDN
                     TYPGLHCDVPSRYYTYSFRPNPEWSRWQSPGPEILEYFRQVAQERHIRPHIRFDTEVK
                     SAQYRDGQWWLTTAAGEEPFDVLVTATGFLRVPKYPDIPGRETFAGPAFHSSRWDHSV
                     SLPDKRIGLIGTGSTGVQITSELGGKVRQLKVFQRTAQWIAPWPNLRYSALTRAVLTR
                     WPRLSNPSYRFWGWFMRYGFGHATVQPGWQRRLYSAQCRWNLRLSVRDPELRRKLTPK
                     DQPLCKRLVIAGHYYQAVQEPRVEVITDAIDHIEPGGVVTKDGILHELDLLIFATGFD
                     ARAYVRPMEIIGENGVALNDVWADGPHAYRSVAVPGFPNLFMLMGPHSPIGNQSLVYV
                     AEDQADYATWWIKQIRDGRIVAAAPTEAATKEYNESMKAAMPDTIWVSGCNSWYLGKD
                     GLPEAFPWIPERHRELLMERNVTDFEVRTA"
     gene            3022..4035
                     /locus_tag="VF874_02390"
     CDS             3022..4035
                     /locus_tag="VF874_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HEX9174972.1"
                     /translation="MKRVVVARYGGPEVLGVVDEDEPTPGPGEVCVRVLAAGVSFTDT
                     LLRAGTYPGVPKPPFTPGYEFVGVVEKLGPGCSALTPGDWVAALVVWGGYAEIVCVAE
                     SLAIKAPDDVDPAVLVSLIFPYMTAYQLIHRAARARPGEVVLYHGAAGAVGVALLELA
                     EPAGLRVFGTASTSDCALVERLGGVAIDYTTDDFLTHVRQLTPDGVDVAIDGIGGSLS
                     FRSFRALRRGGRLVLFGHYSTVSHGRRSRRGETILYAWAAAVMLMGLLSPNRRVMTYR
                     IAKLRDRHPDWFSDDLQELIRLLREDKLHPAVAERIPLAQARRAHELLEHTAVKGKLV
                     LIP"
     gene            4071..7643
                     /gene="cobN"
                     /locus_tag="VF874_02395"
     CDS             4071..7643
                     /gene="cobN"
                     /locus_tag="VF874_02395"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX9174973.1"
                     /translation="MPESTTVLLLSTSDTDLITARASHAAYRWANPSRLALDELPDLL
                     HGVDVVVVRILGGYRSWQDGIDAVTASGVPAVLVSGEQAPDAELMDRSTTPAGIAVQT
                     HLYLAHGGVDNLRQLHAFLSDTVLMTGYGFAPPVSSPNWGVLERASTNDDGPTIAVLY
                     YRAQHLAGNTDYVDALCNEIERAGGRALPVYCASLRTADPELIDTLEAADAMVVTVLA
                     AGGAVPSTAAAGGDDDSWNVAHLAALDIPILQGLCLTNPRAAWESNDAGLSPLDVATQ
                     VAVPEFDGRIITVPFSFKEIDDEGLISYVPDAERCARVAGIALRQARLRRIAPEDKRV
                     AVVFSAYPTKHARIGNAVGLDTPASAIALLQAMRQRGYRLGDIPGLDTEDGDALVHAL
                     IERGGQDPDWLTAGQIEGNPIRVPARQYREWFETLPAEFTDRVQQHWGAPPGELFVDR
                     SQDPDGEIVVAAMQSENVVLMVQPPRGFGDKPVAIYHDPDLPPSHHYLAAYHWLTAGF
                     KADAIVHLGKHGNLEWLPGKTLGMSAACAPDAALGDLPLIYPFLVNDPGEGTQAKRRA
                     HAVLIDHLIPPMARAETYGDIARLEQLLDEHANIAALDPAKLPAVRQQIWTLLQAAKM
                     DHDLGLTDRPADEQFDDMLLHVDGWLCEIKDVQIRDGLHILGQPPEGEHQLDLVLAIL
                     RARQLWAGDQSLPGLREALGLAEDCTDRAAVDAAEADARALVAALQKADWDPAAVDAL
                     TDDPQVAAIMRFAATEVVPRLAGTAAEIDQVLRALDGRFIAAGPSGSPLRGLVNVLPT
                     GRNFYSVDPKAVPSRLAWETGAALADSLLVRYRDDFGHWPKSVGLSVWGTSAMRTAGD
                     DIAEVLALLGIRPIWDDASRRVTGLEPMALAELGRPRIDVTVRISGFFRDAFPHVVTM
                     LDDAVRLAAGLDEPAEHNYVRAHAQADLAEHGDERRCTTRIFGSKPGTYGAGLLQLID
                     SRAWRTDADLAEVYTAWGGFAYGRGLDGAPASDDMQRQYRRIAVAAKNTDTREHDIAD
                     SDDYFQYHGGMVATVRALTGRDPAAYIGDNTTPESVRTRSLSEETARVFRARVTNPRW
                     MAAMRRHGYKGAFEMAATVDYLFGYDATAGVVADWMYERLTEQYVFDDVNRKFMAESN
                     PWALHGMAERLLEAVSRGMWAKPDPQAVDGLKRVLLETEGDLEG"
     gene            7846..8229
                     /locus_tag="VF874_02400"
     CDS             7846..8229
                     /locus_tag="VF874_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX9174974.1"
                     /translation="MPTTFDEVAQSKFILLTTFTKHGRPKPTPIWAAPDGDRLLVITE
                     EDSWKVKRIRNTARVTLAVCDAKGTPKSEAIDATATILDKSQTGLVYDAIGERYGLMG
                     KVFNLFSKYFRGGMKKNVGLELKAA"
     gene            8232..8615
                     /locus_tag="VF874_02405"
     CDS             8232..8615
                     /locus_tag="VF874_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX9174975.1"
                     /translation="MAPTFDDVAKSEYVLLTTFTKDGRPKPSAVWAVRDGDRLRIITD
                     DESWKVKRIRNTPRVTIAKSGGLGKPKSEPVEAVARVLPKSETRAVYNAVLKRYWYHA
                     WWFHAHTLVRGGIDQVHVAVEVKPA"
     gene            8649..9077
                     /locus_tag="VF874_02410"
     CDS             8649..9077
                     /locus_tag="VF874_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX9174976.1"
                     /translation="MVLRWFLVYAVVELAALVALAWTIGFGWTVLLLLATLIVGIALA
                     GSQVKRQIRRFRGGLSDPQGALTDSALIALGTLLVVVPGIVTTALGLLLLIPPTRAAA
                     RPVVAALAAKRAPLVMVVRRDYIDGEVVDVQDFEPPALPR"
     gene            9074..10663
                     /locus_tag="VF874_02415"
     CDS             9074..10663
                     /locus_tag="VF874_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9174977.1"
                     /translation="MTQLLIRGRIHSPAHPDATAMAVRDGVVAWLGSDDVGRAQFRDA
                     EIIDLDGAFVAPAFVDSHVHMTATGLTLTGLDLRAATSRQHCLQLLSDYARAHPDGPI
                     WAHGWDESAWPQPLAPTTDDVDATVGDRPAYLARVDVHSAVVSTALRRMAPNLNGCNG
                     FHPQHPVTADAHHDVRAAARSLVLRDQRTQARVAALDAAAALGIVAVHECAGPDIGGL
                     DDWQELRGTDHGVDVVGYWGQAVTTADEATDLLASTGARGLAGDLFVDGALGSRTAWL
                     SEAYADAGHCGHSYLDPEAITAHVLACTEVGIAAGFHVIGDAAVTAVVDAFAAVVDRC
                     GVAAVSRCGHRLEHLEMVSDEQAHRLGSWGVIASVQPNFDALWGGDAGMYARRLGAVR
                     GRRLNPLALLASSGVPLAFGSDTPVTSMNPWATVRAATHHRTPGSAVSVRAAFVAATR
                     GGWRAAGVRDGVAGTLVPGAPASYAVWDAGPLAVNAPSDAVQRWSTDPRSRVPALPRL
                     DDADPLPRCLRTVHRGEVIHG"
     gene            10656..12290
                     /gene="lnt"
                     /locus_tag="VF874_02420"
     CDS             10656..12290
                     /gene="lnt"
                     /locus_tag="VF874_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9174978.1"
                     /translation="MADAATETKVARLGRGILDRLPEMGVTIAAGILLCVSYPPFGWW
                     YCAVVAFAVLAWVLLRESTSLLGGLGYGFLFGLAFYIPLLPWTGELVGTFPWLALAAL
                     QAVFPAAFGVAAVAVRRLRGWPIWFAALWVAAEWLKSSVPFGGFPWGVVAFGQTGGPM
                     LPLVRLGGVPLLSFAVTLLGFSVTALVLEFVHWWGNRGDTPPRVVLPGACIAVVLFAI
                     AATWPGVRQSGTGAGNDPVINVAAIQGNVPRLGLDFNAQRRAVLDYHVRETLHLADDV
                     RAGRAAQPQLVIWPENSSDVDPLRNPDAAQQISTAAAAIRAPILVGAVLQSPDYSADN
                     PAATNTVIVWDPARGPGERHDKQIVQPFGEYLPWRGLFSHLSPYAERAGYFVPVDGDG
                     VVHAAGVPVGVTTCWEVIFDRAARESVVNGAQLLAVPTNNALFGQSMSEQQLAFAKVR
                     AVEHDRYAVVAGTTGISAIIAPDGHEVARTNFFEPAYLDKQVHLKTDVTPATRWGPIV
                     AWVLIGIGGAALVVAILHNGWFSRRLRSRGTDKGAT"
     gene            12287..13102
                     /locus_tag="VF874_02425"
     CDS             12287..13102
                     /locus_tag="VF874_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX9174979.1"
                     /translation="MTTPGGGAQRPGESAGPAGDRPSRRTLAIIPTYNERENLPLIVQ
                     RLQNALPDAHILVVDDGSPDGTGELADQLASADPDRIHVMHRTAKDGLGAAYLAGFAW
                     GLSRQYDVLVEMDADGSHAPEQLSRLLDAVDAGADLAIGSRYVPGGEVRNWPWRRLVL
                     SRTANWYSRTLLGARVNDITAGYRAYRREVLEKIDLSAVESKGYCFQIDLTWRTINNG
                     FTVAEVPITFVERELGVSKMSGSNIREAIVKVAQWGVRGRMDRSRDSAPAERR"
     gene            complement(13104..13442)
                     /locus_tag="VF874_02430"
     CDS             complement(13104..13442)
                     /locus_tag="VF874_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEX9174980.1"
                     /translation="MADRVLRGSRLGAVSYETDRNHDLAPRQIARYRTENGEVFEVPF
                     ADDAEIPGTWLCRNGMEGTLIEGDLPEPKKTKPPRTHWDMLLERRSTEELDELLKERL
                     DIIRQRRRGS"
     gene            13714..13980
                     /locus_tag="VF874_02435"
     CDS             13714..13980
                     /locus_tag="VF874_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174981.1"
                     /translation="MGRRRQYCRQSCRQRAYEHRAAVKGTALPTDAVVLSADEAARLS
                     DRVYQLRCAAEDVATAVDEGAAKSELRELCGALLDAAKAADGWR"
     gene            14000..14353
                     /locus_tag="VF874_02440"
     CDS             14000..14353
                     /locus_tag="VF874_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9174982.1"
                     /translation="MNADNQSGGLPLADDHAHLVVEVFRMLADATRVQLLWALADDEL
                     SVNDLAERIGKPGPSVSQHLAKLRMARLVRTRREGTQVFYRLENDHVRQLLTDAVYNA
                     EHAAGGTPAHHRRGA"
     gene            14353..15276
                     /locus_tag="VF874_02445"
     CDS             14353..15276
                     /locus_tag="VF874_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016881499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9174983.1"
                     /translation="MGVGHSHDHADHVDDALTTSAAGIRAVKISLLALAATAIAQLAV
                     VALSGSVALFADTVHNFSDALTAVPPWIAFALGRRAATRRYPYGLGRAEDLAGLFVVA
                     VIAASALVAAVQAVHRLIEPVSLNHLSLVAAAGVVGFVGNELVAIYRIRVGTRIGSAA
                     LRADGLHARVDGLTSLAVVLGAGGVAAGFPAADPIVGLIIAVVIAGVLAVAARDVFRR
                     LLDGVDPGLIDTACDVLARQDGVRGVRRVRMRWLGHRLVADAELDIDPQQSLADAHRT
                     AHEAEHALIHAVPKLAEATIHAYPAHLAVSS"
     gene            complement(15266..15940)
                     /locus_tag="VF874_02450"
     CDS             complement(15266..15940)
                     /locus_tag="VF874_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9174984.1"
                     /translation="MRSWAAILAALVLVAGCSETVGGQSQPAKLTPLPSSPSKSTTTS
                     APVPTVAPEPGAAIADVTAWIAAGEPADEAAFHTATRDGTETQLGDDVAFTTPSGKTR
                     CTTYAKFTGALMCLVQLKNPPPRPPDFPTAWVGGWVNFDGKSLSVGSPHGDPGPFVNG
                     DGPKLPYGSTLKFGEYQCRSDPVGLYCTTDAHESAVRYSDAGIEPFGCLDPVTPPADI
                     GLQFSC"
     gene            16034..17539
                     /locus_tag="VF874_02455"
     CDS             16034..17539
                     /locus_tag="VF874_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEX9174985.1"
                     /translation="MADGTVRVTTTSGTVEGVTRSGVTKWRSIPYARPPVGRLRYRAP
                     QPAIPWRGVRYCHDFAFCAPQRRRYTLIGVNKYQPMSEDCLTVNVVAPASPPAEPLPV
                     MFFIHGGGYMLGSSATPLYDGAALARRGCVFVSANYRLGALGCLDLSWLSTPDHPIES
                     NLFLRDLVLALEWVRDNIGAFGGDPDRVTIFGESAGAHAVATLLAVPAARGLFAGAIS
                     ESPANGMVVPGEVAPRFAAQFVEFLGVEERAAAATLMQAEPRRLVKALDQLLTHASQE
                     MSAGSFAIGPTVDGDYLPRDPFIAMARGEAHRVPVIVGHNAEESRLFTRWLKLLPTTE
                     HMLERLLAGIDPASRERIQIAYPGYPHPDECMRLSADMFFASAAWRIAEAHSKHAPTY
                     MYRYDYAPRTLTWSGLGATHAMELLAVFDIYRTRLGSLLTVAADQRSALRVSNNIQSR
                     WRRFSRTGVPGTGWPTYDHDRAVLVFDKHSRVEYDPHPARREAWENFSLVR"
     gene            17556..18740
                     /locus_tag="VF874_02460"
     CDS             17556..18740
                     /locus_tag="VF874_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate 2-monooxygenase"
                     /protein_id="HEX9174986.1"
                     /translation="MADTAPQAPFGNYQYEVYLKGMAGQAPDFPMSWTELEVQASAAM
                     DAGANGYVFGSAGTEDTERENRAAFTRHRIVPRMLRDVSTRDLRTEMFGVQHAAPVLL
                     APVGVQSIVHPDGELAAARGAAEAGIGFVASTASSYSMEDIAAAGGDVPRWYQLYWPK
                     DPDLTASFVARAEAAGYGALVVTLDTGLLAWRPRDLQTAYLPFLQSVGIANYLSDPVF
                     RAALQKSPEDDPTAAVLQFVGVFSNPAMGWGDLAALRELTDMPIVLKGILHSDDARLA
                     RDHGVQGVVVSNHGGRQVDGAIAALDALPDVVDAVGDELTVLFDSGIRSGADVVKALA
                     LGARAVLLGRPYLWGMALRGADGVAQVVRNLLSELDLTLALSGHASPRTLNREVLRNR
                     GA"
     gene            18751..>19304
                     /locus_tag="VF874_02465"
     CDS             18751..>19304
                     /locus_tag="VF874_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9174987.1"
                     /translation="MRAADLSAEWLSAQLNLPVSSFETDRIGTGQMSECYRVVPHYGG
                     DTMGPSSVVLKVAASDPASRQTGFALGLYEREVRFYAEVAPRLVGPIAPCYHASFDAD
                     AGTFALLLGDAGPAEVGDEIEGTSVERARLAVSELGRLHGSAIADPTLAGAAWLNRES
                     PVSQALLTQLYAGFTERYADRIAAR"
BASE COUNT         2954 a         6575 c         6670 g         3105 t
ORIGIN      
        1 tcgtcgcggt cgacgtcctc cgcggcgcac cgttcgagac cgagcaggac ccgatcatca
       61 cgcggatgtg ggccctgcag cggtccgcga tgtatcgcga catgggcgtg atcggcgtca
      121 acgtcgtgtc gtggcccgag gacgtcacgc tggaccagtc gatgcgactg gtgcccgatc
      181 acctgcacgg cgcggggcgg cgctgacatg acgtcttcga tgcagcctgc gcctctcgcg
      241 ttttcgaccg cgttcggctt gctgatggtc gccgccgccg cgatcgacgc cgacagggcg
      301 ggagccgtcg cggtcgcagt ggctgtcggc gctctgctta ttggccttcg atatcgtgct
      361 gcggcaacgc tttcggtgtt gcttgtcgtg ttcgcgatcg tgctctccga tccgccggcg
      421 ctgtacgcgg cactggccgg gctgtcggcg gcggcctacc tggtgctgcg atacgccgca
      481 tcactcggtg tcgtcaccac cacccggtcg acggtgatcg ggacagtcgg cttcacgctc
      541 gtcgggatcg ctgccaccgt tgtgccggtg agcgtgccgt ggctgccgtt ggttgcgccg
      601 gtcgctgcgg tggccgtttt cctggtggcc gcgctgccgt taatgcgcgg tctcagcgac
      661 tgactgggaa tatcgcatca ccgaagtgcg agagaaggcg cgcgaccggc acgtcgacgt
      721 tgtgtgaggc cgccagcgct accgcatcgg ccaagtcctt ttcgagcagc gccgcggcga
      781 cccgtagttt ttcgtcctcg tcggcgccgt gcgggtcgtc gcggaagtgt ttgtagaacg
      841 gcagtacaaa cgcgctcagc gacgaaagcg caccgctggc ctgcgcgacc tcctccacaa
      901 ggccgtcgcg cgcgccggca gcccgggcga gctcgacggc ctcgatcatc gccgcgaagc
      961 cggcatagac cacgatgttg tgagccaact tcagtgctgc gccgctgccg actcggcctg
     1021 ccggcacgac cttcgacgcg aagcggcgca gcgtgggctc caggcgggtg acgagttcac
     1081 cgtcgtcgcc caccatcacc gacatcgtgc cctcttcgac gctgaacatg ccccggccgg
     1141 cgatcggggt gtcgacgaga tgcacgtcct tttggccggc cgtcgcggcg aggctttcga
     1201 tggtcgacgg cgcgaccgtc gagagcacgg cgatcaccgt gccgggtttg gcgccttcga
     1261 gcgctccact ttcaccgcag accgcggcga cgcactgatc gtcggtctga acgcagacga
     1321 gcacgatgtc acagcgctcg gcgagcccag cggaggagcc ggcagggatg gcaccctgtg
     1381 aagccgcggc ttcgaccgct tccggattca cgtcgaacac aagcggcgga tcaccggttt
     1441 cgatgagccg gtgcgccagc gcgctgccga tcaaacccag cccgatgacg ccaactgatg
     1501 tcatgcggtc agtaatacta gattgcacca cgtgtgggcc agaaactctg cgcgaccggc
     1561 gtagggtctg cgcatgacca cagtcgcgat catcggcgcg ggcatgtcgg ggttgtgcat
     1621 ggcgatcaag ctgcaagagg ccggcatcga gtcgtacacg atcttcgagc aggcgtccga
     1681 tgtcggcggc acctggcggg acaacaccta tccggggctg cattgtgacg tgccgtcgcg
     1741 ctactacacc tattcgtttc ggccgaaccc cgagtggtct cgatggcagt ctccgggtcc
     1801 cgaaatcctg gagtacttcc gacaggtcgc ccaagaacga cacatcagac cgcacatccg
     1861 gttcgacacc gaggtgaaat ccgcccagta ccgcgacggt cagtggtggc tgaccacggc
     1921 cgccggtgaa gagccgttcg acgtgctcgt caccgcgacc ggcttcctga gggtgccgaa
     1981 gtaccccgac atccccggcc gtgagacgtt cgccggtccg gcgttccatt cctctcgctg
     2041 ggatcactcg gtgtcgttgc cggacaagcg gatcggtctg atcggcaccg gatcgaccgg
     2101 tgtgcagatc acgtcggaac tcggcggaaa agtgcggcag ctcaaggttt ttcaacgcac
     2161 cgcacagtgg atcgccccat ggcccaacct gcgttattcg gcgctgacgc gcgcagtgct
     2221 gacgcgttgg ccacgtctca gcaacccgag ctaccgtttc tggggatggt tcatgaggta
     2281 cgggttcggc cacgcgaccg tgcagccggg ctggcagcgg cggctctaca gtgcgcaatg
     2341 ccgctggaac ctcaggctct ccgtgcgcga cccggagctg cgcaggaagc tcaccccgaa
     2401 ggaccagccg ctgtgcaagc ggctggtgat cgcgggccac tactaccagg ccgtgcagga
     2461 gccccgcgtc gaagtgatca ccgacgcgat cgaccacatc gagccggggg gcgtcgtcac
     2521 caaggacggc atcctgcacg agctcgatct gctgatcttt gcgacgggtt tcgacgcccg
     2581 ggcgtacgtg cgtccgatgg agatcatcgg cgagaacggg gtcgcgctca acgacgtctg
     2641 ggccgacggg ccgcacgcct accgctcggt ggcagtgccg ggtttcccga acctgttcat
     2701 gctgatgggt cctcattcac ctatcgggaa ccagtcgttg gtctacgtcg ccgaagatca
     2761 ggcggactac gccacatggt ggataaaaca gattcgcgac gggcgcatcg tcgccgccgc
     2821 gccgaccgaa gcggccacca aggaatacaa cgagagcatg aaagcggcta tgccggacac
     2881 gatctgggtc agcggctgca acagctggta tctcggcaag gacggcctgc cggaggcgtt
     2941 cccctggatc ccggagcggc atcgcgaact gctcatggag cgcaacgtca ccgacttcga
     3001 ggtccgcacc gcataaccaa gatgaagcgc gtcgtcgtcg cccgctacgg aggccctgag
     3061 gtcctcggcg tcgtcgacga ggacgaaccc acaccaggtc cgggagaagt ctgcgtccgc
     3121 gtactggccg ccggtgtgtc cttcactgac acactgcttc gtgccgggac ttatcccggg
     3181 gtgcccaaac cgccgttcac tccgggatac gagttcgtgg gcgtcgtcga aaaactaggc
     3241 cccggatgct cggcgctgac gccgggagac tgggtcgctg cgctcgtcgt gtggggcggg
     3301 tatgccgaaa tcgtatgtgt cgcagagtca ctggcaatca aggcgcctga cgacgtagac
     3361 ccggcggtcc ttgtcagcct gatcttcccg tacatgaccg cctaccagct gatccaccgc
     3421 gcggccagag cccggccggg cgaggtggtg ctctaccacg gggcagcggg ggcggtgggt
     3481 gtcgcgctgc tcgagctggc cgagccggcc ggtctgagag ttttcgggac cgcgtcgacg
     3541 tcggactgcg cgctcgtgga gcggctcggc ggcgtggcca tcgactacac gaccgacgac
     3601 ttcctgacgc acgtgcgcca gctgacaccc gacggcgtcg atgtcgcgat cgacggcatc
     3661 gggggatcgt tgtccttccg gtcatttcgc gcattgcggc gcggaggtcg gctcgtactc
     3721 ttcggccatt actcgacggt ctcgcacggc cgtcggagcc ggcgcgggga gaccatcttg
     3781 tacgcctggg cggcggcggt gatgctgatg ggcctgctgt caccgaaccg gcgtgtgatg
     3841 acctaccgga tcgcgaagct gcgggaccgt catcccgact ggttcagcga cgaccttcag
     3901 gaactgatcc ggctcttgcg cgaggacaag ctgcaccccg ccgtcgccga gcgcattccg
     3961 ttggcgcagg cgcgccgtgc ccacgaactc ctcgagcaca ccgccgtcaa aggcaagctt
     4021 gtgctgatcc catgaccacc gggtgatcgg cgctgcggta cgaatgacgg gtgcctgagt
     4081 cgaccaccgt cctgctgctg tcgacgtccg acaccgacct gatcaccgcc cgcgccagcc
     4141 acgcggcgta ccggtgggcg aacccgtcgc ggctggccct cgacgagctg cccgacctgc
     4201 tccacggggt cgacgtcgtc gtcgtgcgca tcctcggcgg ctatcgctcc tggcaggacg
     4261 gcatcgacgc ggtgaccgcc agcggggtcc ctgcagtgct ggtgagcggc gagcaggccc
     4321 ccgacgcgga gctgatggac cgctccacca cgcccgccgg gattgccgtg cagacccacc
     4381 tgtacctggc ccacggcggc gtcgacaacc tgcgccagct gcacgcgttc ctgtccgaca
     4441 ccgtgctgat gaccggctac ggcttcgcgc cgcctgtcag ctccccgaac tggggtgtgc
     4501 tcgagcgggc gtcaacaaat gatgacgggc ccacgatcgc ggtgctctac taccgcgcac
     4561 agcatctggc cggtaacacc gactacgtcg acgcgctgtg caacgagatc gagcgcgccg
     4621 gcggccgcgc gctgccggtg tattgcgcat cgctgcgcac cgccgatccc gagctgatcg
     4681 acacgctcga agccgccgac gcgatggtgg tgaccgtgct ggccgccggg ggagcggtcc
     4741 cgtcgacagc ggcggccggt ggtgacgacg acagctggaa cgtcgcgcac ctcgccgcgc
     4801 tcgacatccc gatcctgcag gggctgtgcc tgaccaaccc ccgcgccgca tgggaatcca
     4861 acgacgccgg gctcagcccg ctcgacgtcg ccacccaggt ggccgtgccc gagttcgacg
     4921 gacgcattat cacagttccg ttctcgttca aggagattga cgacgagggc ctgatctcct
     4981 acgtgccgga cgccgagcgc tgcgctcgtg tcgcggggat cgcattgcgg caggcccggc
     5041 tgcgccgcat cgcaccggaa gacaagcggg tggccgtcgt gttctccgcg tacccgacca
     5101 agcacgcgcg catcggcaac gccgtcggcc tggacacccc cgcaagcgcg atcgcgctgc
     5161 tgcaggcgat gcgccagcgc ggataccggc tcggcgacat cccgggtctc gacaccgaag
     5221 atggggacgc gctggtgcac gcgctgatcg aacgcggggg acaggacccg gactggctga
     5281 ctgcaggcca aatagagggc aacccgattc gcgtgccggc gcggcagtac cgggaatggt
     5341 tcgagacgct gccggctgaa ttcaccgatc gcgtgcagca gcactggggc gcgccgccgg
     5401 gggagttgtt cgtcgaccgc agccaggatc ctgacggcga aatcgtggtt gcggcaatgc
     5461 aatccgaaaa cgtcgtgctg atggtgcagc cgccgcgcgg attcggcgac aagccggtcg
     5521 cgatctacca cgacccagac ctgccgccaa gtcatcacta cctggcggcg taccactggc
     5581 tcaccgccgg gttcaaggcc gacgcgatcg tgcacctcgg caagcacggc aacctcgagt
     5641 ggctgcccgg caagacgctc ggcatgtctg cggcgtgcgc acccgacgcg gcgctgggcg
     5701 atctgccgct gatctacccc ttcctcgtca acgaccccgg cgaaggcacg caggccaaac
     5761 gccgcgcgca cgccgtgctc atcgaccacc tgatcccgcc gatggcgcgc gccgaaacct
     5821 acggcgacat cgcgcgattg gagcagctgc tcgacgagca cgccaacatc gccgcgctgg
     5881 acccggcgaa gctgccggcc gtgcgccagc agatctggac gctgctgcag gccgcgaaga
     5941 tggaccacga cctggggctg accgatcggc ccgccgacga gcagttcgac gacatgctgc
     6001 tgcacgtcga cgggtggctg tgcgaaatca aggacgtcca gatccgcgac gggctgcaca
     6061 ttctcgggca gccgcccgaa ggcgagcacc agctggacct cgtgctcgcc atcctgcggg
     6121 cccgccagct ctgggccggc gaccagtcgc tgcccggcct acgggaggcg ctcgggctcg
     6181 ccgaggactg caccgatcgc gccgccgtcg acgcggccga ggccgacgcc cgggcgctgg
     6241 tcgccgcgct gcagaaggcg gactgggacc cggcggccgt cgacgcgctg accgacgacc
     6301 cgcaggtggc ggcgatcatg cggttcgcgg ccaccgaggt ggtgccgcgc ctcgcgggca
     6361 ccgcggccga gatcgaccag gtgctgcgtg cgctggacgg ccggttcatc gccgcggggc
     6421 cgtccgggtc gccgctgcgc gggctcgtca acgtgctgcc caccggccgc aacttctatt
     6481 ccgtcgaccc gaaggcggtg ccgtcgcggc tggcgtggga aaccggtgcg gcgctggctg
     6541 attcgctgct cgtccggtac agagacgact tcggccactg gccgaagtca gtgggactgt
     6601 cggtgtgggg cacgtcggcg atgcggaccg ccggcgacga catcgccgaa gtgcttgcgc
     6661 tgctgggcat tcgaccgatc tgggatgacg catcgcggcg ggtgaccggg ttggagccga
     6721 tggcgctggc agagttgggc cggccacgga tcgacgtcac cgtccgcatc tccggctttt
     6781 tccgcgacgc gttcccgcac gtggtcacga tgctcgacga cgcggtgcgg ctcgccgcag
     6841 gcctggacga gcccgcagaa cacaactatg tacgcgcgca cgcgcaggcc gacctcgccg
     6901 agcacggcga cgaacgacgc tgcacgacaa gaattttcgg atcgaagccc ggcacctacg
     6961 gcgcgggtct gctgcagctg atcgacagcc gggcctggcg caccgacgcc gacctcgccg
     7021 aggtgtacac ggcgtgggga ggtttcgcgt acggccgcgg cctcgacgga gcgccagcct
     7081 cagacgacat gcagcgtcag taccggcgaa ttgcggtggc ggccaagaac accgacaccc
     7141 gcgagcacga catcgccgac tccgacgact acttccaata ccacggcggc atggtggcca
     7201 cggtccgcgc gctgaccggg cgcgacccgg ccgcctacat cggcgacaac accaccccgg
     7261 aatcggtgcg cacccgcagc ttgtcggagg aaacggcgcg ggtgttccgc gcgcgggtga
     7321 ccaacccgcg gtggatggcg gcgatgcgca ggcacggcta caagggtgcg ttcgagatgg
     7381 ccgcgaccgt cgactacctg ttcggctacg acgcgaccgc cggcgtggtg gccgactgga
     7441 tgtacgagcg gctcaccgag cagtacgtgt tcgacgacgt caaccgcaag ttcatggccg
     7501 agtcgaaccc gtgggcgctg cacgggatgg ctgagcggtt gctcgaagcg gtgagccgcg
     7561 gcatgtgggc gaaaccggac ccccaggccg tcgacgggct caagcgggtc ctgctggaga
     7621 ccgagggcga cctcgaggga tgatggttta gtgaccgagc cgccgcgccg agacgcagcc
     7681 gtggttcgca gttgccgcat actcgaagcc gaggtgtggc cgatcaccgc tgggaccagc
     7741 ccgatcacca aagcggagcg cgagaacata ctcggttacc gcgagaccgg cgtctgtgtg
     7801 gttggccggt gggatggcaa ctgctgacct ctagggtcga tagctatgcc cacgacgttt
     7861 gatgaagtcg cgcagtccaa gttcatcctg ctgaccacgt tcaccaagca cggacggccc
     7921 aagccgacgc cgatatgggc cgcgcccgac ggcgaccggc tgctggtgat caccgaggaa
     7981 gactcgtgga aggtcaagcg gatccgcaac actgcgcgcg tgacgctcgc cgtgtgcgat
     8041 gcgaaaggca ccccgaagag cgaggctatc gacgccaccg cgacgattct cgacaagtcg
     8101 cagacgggcc ttgtgtacga cgcgatcggt gagcgatacg ggctgatggg caaggtgttc
     8161 aacctgttca gcaagtactt tcgcggcggg atgaagaaga acgtcggcct cgaactgaag
     8221 gcggcgtagc cgtggcgcca acattcgacg acgtcgccaa gtcggagtac gtgttgctga
     8281 cgacgttcac gaaggacggc cggcctaagc cgtcggcggt ctgggcggtc cgcgacggcg
     8341 atcggctgcg gatcatcacc gacgacgaat cgtggaaggt gaagcggatc cgcaacaccc
     8401 cgcgggtgac gatcgccaag agcggcggac ttggcaaacc caagagcgag cccgtcgagg
     8461 cggtggcgcg cgtgctgccg aagtccgaga caagggctgt ctacaacgcc gtcctgaagc
     8521 gctactggta tcacgcctgg tggttccacg cgcacacgct ggtgcgcggc ggcatcgacc
     8581 aggtgcacgt cgccgtcgag gtcaagcccg cgtaggaacc ctgaccggtc gcgatgcgtt
     8641 tccctcacat ggtcctgcgg tggtttctgg tctacgcggt cgtcgagctg gcggctctcg
     8701 tcgcgttggc atggacgatc ggattcggtt ggacggtcct gttgctgctc gccacgctga
     8761 tcgtcgggat cgcgctggcc ggctcgcagg tcaaacggca gatccgtcgc ttccgaggtg
     8821 gattgtccga tccgcaaggg gcgctcaccg acagtgcgct gatcgcgctc ggcaccctgt
     8881 tggtcgtcgt acccggaatc gtcaccaccg cgctgggtct gctgttgctt atcccgccga
     8941 cccgagccgc cgctcgtccg gtagtcgcag cgcttgccgc caagcgcgcg cctctggtca
     9001 tggtggtccg gcgtgactac atcgacggcg aggtcgtcga cgtccaggat ttcgagccgc
     9061 ccgcgctccc tcggtgacgc agctgcttat ccggggccgg atacacagcc cggcccaccc
     9121 cgacgccacc gcgatggccg tccgcgacgg tgttgtcgcg tggctgggca gcgacgacgt
     9181 cggacgcgct cagttccgcg acgccgagat catcgacctg gatggcgcgt tcgtcgcgcc
     9241 ggcgttcgtc gacagccatg tccatatgac cgccaccggc ctgacgttga cgggtctgga
     9301 cctgcgcgcc gccacctcgc ggcagcactg cctccagctc ctcagcgact acgcccgcgc
     9361 acaccccgac gggccgatct gggcgcacgg gtgggatgag tcggcgtggc cgcagccgct
     9421 cgctccgacc accgacgacg tcgacgccac cgtcggtgac cggcccgcct acctggcccg
     9481 cgtcgacgtg cactcggcgg tcgtctccac ggcgctgcgc cgcatggcgc caaacctgaa
     9541 cggctgcaat ggctttcacc cgcagcaccc ggtcaccgcc gacgcgcatc acgacgtgcg
     9601 cgccgccgcg cgcagcctgg tcctcagaga tcagcggacc caggctcggg tcgccgcact
     9661 agatgccgcg gccgcactgg gcatcgtcgc cgtgcacgag tgcgcggggc ccgacatcgg
     9721 tggactcgac gactggcagg agctgcgcgg caccgaccac ggcgtcgacg tcgtcggcta
     9781 ctggggccag gccgtcacga ccgccgacga agccaccgac ctcctcgcca gcaccggagc
     9841 ccgcggcttg gccggcgacc tgttcgtcga cggcgcgctc gggtcccgca ccgcctggct
     9901 cagcgaggcg tacgcagacg ccgggcactg cggccacagc tacctggacc ccgaagcgat
     9961 caccgcccat gtgctcgcgt gcaccgaggt cggcatcgcg gccggcttcc acgtgatcgg
    10021 cgacgccgcg gtcaccgcgg tcgtcgacgc gttcgccgcc gtcgtcgacc gatgtggcgt
    10081 cgccgcggtc tcccggtgcg gacaccgcct ggaacacctc gagatggtca gcgacgagca
    10141 ggcccaccgg ttgggttcgt ggggcgtgat cgccagcgtg cagccgaact tcgacgcgtt
    10201 gtggggcggc gacgccggca tgtacgcccg tcgactgggc gccgtgcgag ggcgccgatt
    10261 gaacccgctg gcgctgttag catcgtcggg cgtgcccctc gccttcggct ccgacacccc
    10321 ggtgaccagc atgaacccgt gggcgaccgt gcgggcagct acacaccacc gaacgcccgg
    10381 cagcgccgtc tcggtgcgcg cggcgttcgt ggcggccacc cgcggcggct ggcgcgcggc
    10441 gggggtgcgc gacggcgtcg ccggaacgct ggtgcccggt gcgcccgcgt cgtacgcggt
    10501 ctgggacgcc gggccgctgg ccgtgaacgc gccgtccgat gcggtgcagc gatggtccac
    10561 ggacccgagg tcgcgggtgc ctgcgctgcc acggctggac gacgccgatc cgctgccgcg
    10621 gtgcctgcgc accgtgcacc gcggtgaggt catccatggc tgacgctgcc accgagacga
    10681 aggtcgctcg gctgggccgc ggcatcctcg accgactgcc cgagatgggc gtgacgatcg
    10741 ccgcgggcat tttgctgtgc gtgagctacc cgccgttcgg ctggtggtac tgcgcggtcg
    10801 tcgcgttcgc ggtgctggcg tgggtgctgc tgcgcgaatc gacatcgctt ctcggcgggt
    10861 tgggctacgg cttcttgttc ggtcttgcgt tctacatccc gctgctgccg tggacgggcg
    10921 aactcgtcgg cacctttccg tggctggcgc tcgccgccct gcaagcggtc tttcccgcgg
    10981 cgttcggtgt agccgccgtc gccgtcaggc ggctgcgcgg ctggccgatc tggttcgccg
    11041 cgttgtgggt cgcggcggaa tggctgaaat cgagcgtgcc gtttggcgga ttcccttggg
    11101 gcgtcgtggc tttcgggcag accggcggcc cgatgctgcc gctggtgcgg ctgggcggag
    11161 tgccgctgct gtcgttcgcg gtcacgctgc tcggcttcag cgtgaccgcg cttgtgctgg
    11221 agttcgtgca ctggtgggga aaccgcggcg acacccctcc gcgcgtcgtg ctgcctggcg
    11281 cgtgcatcgc cgtggtgctg ttcgcgatcg cggcgacgtg gcccggggtg cgccagtcgg
    11341 gcacaggcgc aggcaacgac cccgtcatca acgtcgccgc gatccagggc aacgtgcctc
    11401 ggctgggcct ggacttcaac gcccagcggc gtgcggtgct ggactaccac gtccgcgaaa
    11461 ccctccatct cgccgacgac gtgcgggcag gacgcgccgc gcaaccgcag ctggtcatct
    11521 ggccggagaa ctcgtcggac gtcgacccgc tccgcaatcc tgatgcagcg caacagattt
    11581 ctactgccgc agccgcgatc cgcgccccga ttctggttgg cgcggtgctg cagagtcccg
    11641 actactcggc ggacaacccg gcggcgacga acacggtgat cgtctgggat ccggcgcgcg
    11701 ggccgggtga acgtcatgac aaacagatcg tgcagccgtt cggcgagtac ctgccgtggc
    11761 gggggctgtt ctcgcatctg tcgccgtacg ccgagcgggc ggggtacttc gtgccggtcg
    11821 acggggacgg cgtggtgcac gcggcggggg tgccggtcgg ggtgacgacg tgctgggagg
    11881 tgatcttcga ccgcgccgcc cgcgagtccg tcgtcaacgg cgcccagctg ctggctgtgc
    11941 cgaccaacaa cgcgttgttc gggcagtcga tgagcgaaca gcaactggcg ttcgcgaaag
    12001 tgcgggcggt ggagcacgac cgctacgccg ttgtggcggg aaccacaggg atcagcgcga
    12061 tcattgcccc cgacggtcac gaggtggcgc gcacgaactt cttcgagccc gcttacctgg
    12121 acaaacaggt gcacttgaag acggatgtga cgcctgccac ccggtgggga ccgatcgttg
    12181 cctgggtgtt gatcggaatc ggtggcgccg ctctggtcgt cgcgatactg cacaatggat
    12241 ggttcagccg tcggctcagg tcgcgcggca cagacaaagg agccacatga cgaccccagg
    12301 gggtggagcg cagcgaccgg gggaaagcgc cgggccggct ggcgatcggc caagccggcg
    12361 cacgctggcg atcattccca cctataacga acgcgagaat ctgccgctga ttgtgcagcg
    12421 gctgcagaac gcgttaccgg acgcgcacat cctcgtcgtc gacgacggca gccccgacgg
    12481 caccggagaa ctcgccgatc agctcgcgtc ggccgatccc gaccggatcc acgtcatgca
    12541 ccgcaccgcc aaggacgggc tcggcgcggc gtaccttgcg ggcttcgcgt gggggttgag
    12601 ccgccagtac gacgtgctcg tcgagatgga cgccgacggc agccacgcgc ccgagcagct
    12661 gtcacgtctg ctcgacgccg tcgacgccgg agccgatctc gcgatcggtt cgcgctacgt
    12721 gccgggcgga gaggtgcgca attggccgtg gcggcgactg gtgctgtcgc gcaccgcgaa
    12781 ctggtactcg cgcacgctgc tgggcgcccg ggtcaacgac atcaccgccg gctaccgcgc
    12841 ctatcggcgt gaggtgctgg agaagatcga cctgtccgcg gtcgagtcga agggctactg
    12901 cttccaaatc gacctgacgt ggcggacgat caacaacgga ttcaccgtcg ccgaggtgcc
    12961 gatcacgttc gtcgagcggg aactgggcgt ctcgaagatg agcgggtcca acatccgcga
    13021 ggccatcgtc aaggtggccc agtggggtgt ccgcgggcgc atggaccgct cccgcgactc
    13081 ggcgcctgcc gagcggcgct aactcagctg ccgcggcggc gctgccggat gatgtccagc
    13141 cgctccttga gcagctcgtc gagctcctcg gtcgaacggc gctccagcag catgtcccag
    13201 tgcgtgcggg gcggcttggt cttcttcggc tccggaaggt cgccctcgat cagcgtgccc
    13261 tccatgccgt tgcggcacag ccaggtgccg ggaatctcgg cgtcgtcggc gaacggaacc
    13321 tcgaatacct cgccgttctc cgtgcggtac cgcgcgatct gacgcggcgc caggtcgtgg
    13381 ttgcggtcgg tctcgtagct cacggcacca agccggctgc ctctcaagac gcgatcagcc
    13441 atcgtcaaca ctctccatcc actgcaggtc cgcgccctat ttttccaggt gaagctccag
    13501 gtaaagcaac gcaggaggcg cactgccagt tcccaggcgc taggcgacgc gacctccagg
    13561 ataccgggct ttggtggcta ggccgtctag agtcgtcagg cggtggatac tcttgctcct
    13621 cgcgtccgcg gtgattctct tgctcctcgc gtccgcggtg cccgccgcgt acagccgtgc
    13681 cgatggtgcg gacgagacgt cgccgacgcc gggatcggcc gcaggcgcca gtactgccgc
    13741 cagtcgtgcc gccaacgggc atacgagcac cgtgccgcgg tgaaaggcac ggcgctgccc
    13801 accgacgcgg tggtgctgtc cgccgacgaa gccgcccggt tgtccgaccg ggtgtatcag
    13861 ctgcgctgcg cagcagaaga cgtcgcgacc gcggtcgacg agggcgcggc gaaatccgaa
    13921 ctgcgagagt tgtgcggggc actgctggac gccgcgaagg cagccgacgg ctggcgctag
    13981 taagcgctat tatctgcgta tgaatgcaga taaccagtca ggcggtctgc cgcttgccga
    14041 cgatcatgcc catctcgtcg tcgaggtgtt ccggatgctc gccgacgcga cgcgcgtgca
    14101 gctgctgtgg gcgctggccg acgacgagtt gagcgtcaac gacctggccg agcggatcgg
    14161 caagccgggg ccgtcggtgt cgcagcatct ggcgaagctt cggatggccc ggctggtgcg
    14221 cacccgccgc gagggcacac aggtgttcta ccggctcgag aacgatcatg tgcgccaact
    14281 gctcaccgac gcggtgtaca acgccgagca cgccgcgggt ggaactccgg cgcaccaccg
    14341 gcgaggtgcc tgatgggtgt tgggcactcc cacgaccacg ccgaccacgt cgacgacgcg
    14401 ctcaccacca gcgccgctgg catccgcgcc gtcaagatca gcctgctggc cctcgccgcg
    14461 accgcgatcg cgcagctcgc ggtcgtcgcg ctgtccgggt ccgtcgcatt gttcgccgac
    14521 accgtgcaca acttctccga cgcgctgacc gcagtcccgc cgtggatcgc gttcgcgctc
    14581 ggccggcgcg cggccactcg gcgctatccg tacgggctcg gccgggccga agacctggcc
    14641 ggcctgttcg tcgtcgcggt gatcgcggcg tccgcgctgg tcgcggccgt gcaagccgtg
    14701 caccggctga tcgagccggt atcgctgaac cacctgagcc tggtcgcggc ggccggcgtc
    14761 gtcggtttcg tcggcaacga gctcgtggcg atctaccgca tccgtgtggg cacccggatc
    14821 ggctcggcgg ccctgcgcgc cgacgggctg cacgcccggg tcgatgggtt gacctcgctt
    14881 gccgtggtgc tcggcgcggg cggcgtcgcg gcggggtttc ccgcggccga cccgatcgtg
    14941 ggcctgatca tcgccgtggt gatcgccggt gtgctggccg ttgccgcgcg tgacgtgttc
    15001 cgccggctac tcgacggcgt ggatcccggg ctgatcgaca ctgcctgtga cgtgctggct
    15061 cgccaggacg gcgttcgggg cgtgcgccgg gtgcggatgc gatggctcgg ccaccgactg
    15121 gtcgccgacg ccgaactcga catcgacccg cagcagagcc tggccgatgc gcaccgcact
    15181 gcccacgaag ccgagcacgc gctcattcac gcggtgccca aactcgccga agcaacgatc
    15241 cacgcgtacc cggcgcacct agcggttagc agctgaactg cagcccgatg tctgccgggg
    15301 gagtcaccgg gtccaggcag ccgaacggct cgatgcccgc gtcgctgtac cggaccgccg
    15361 actcgtgcgc gtcggtcgtg cagtacagcc cgaccgggtc tgaacggcat tggtactcac
    15421 cgaatttcag agtcgagccg tacggaagct tcggcccgtc accgttgacg aacgggcccg
    15481 ggtcgccgtg cggactgccc acggacagcg acttcccgtc gaagttgacc cagccgccta
    15541 cccacgcggt agggaagtcc ggaggcctgg gcggcggatt cttcagctgc accaggcaca
    15601 tcaatgcgcc cgtgaacttc gcatacgtcg tgcacctcgt cttgcccgac ggcgtggtga
    15661 acgcgacgtc gtcgccgagc tgggtttcgg tgccgtctcg ggtagcggtg tgaaacgcgg
    15721 cttcgtcggc cggttcgccc gccgcgatcc acgcggtcac gtcggcgatc gccgcgcccg
    15781 gctccggcgc gaccgtcggc accggcgccg acgtcgttgt ggacttcgac ggcgagctgg
    15841 gcagaggtgt caacttggcc ggctgggact gtccgccgac ggtctcggaa catccggcga
    15901 ccagcaccaa cgcggctagt atcgcggccc aagagcgcat cctgtcaccg tatccggcga
    15961 cggttcgatg agagctgaag ctggcgggcg agtttcaacc agccgaacgc taggggttcg
    16021 ctaccttcaa agaatggccg acggcactgt ccgcgtgacc accacgagcg gcactgtcga
    16081 gggtgtgacc cgcagcggcg tgacgaaatg gcggtcgatc ccgtacgccc gcccacctgt
    16141 ggggcggctg cgataccgtg cgccgcagcc ggcgatcccg tggcgcgggg ttcgctactg
    16201 ccacgacttc gcgttctgcg cgcctcagcg ccgccgctac accctgatcg gggtcaacaa
    16261 gtaccagccg atgagcgagg actgcctgac cgtcaacgtc gtcgcaccgg ccagcccacc
    16321 cgccgagccg ctgccggtga tgtttttcat ccacggcggc ggatacatgc tgggcagctc
    16381 ggcgacaccg ctgtacgacg gcgcggcgct tgcccgccgc ggctgcgtgt tcgtgtcagc
    16441 gaactaccgg ctcggggcgc tcggttgcct cgatctgtcg tggctgtcga cgccggatca
    16501 cccgatcgaa agcaatctgt tcctgcgcga cctcgtgctc gcgttggagt gggtgcgcga
    16561 caacatcggc gccttcggcg gcgacccgga cagggtgacg atcttcggcg agagcgcggg
    16621 cgcccacgcg gttgccaccc tgctggcagt tcccgccgcc cgcgggctgt tcgcgggtgc
    16681 gatctccgaa agtccggcca acgggatggt ggtacccggt gaggtggcgc cacgcttcgc
    16741 cgcccagttc gtcgagttcc tgggtgtcga ggaacgcgcc gccgccgcca cgctgatgca
    16801 ggccgaaccg cggcggctgg tgaaggctct cgaccagctg ctgacccacg cgtcccagga
    16861 gatgtcggcc ggttcgttcg cgataggccc taccgtcgac ggcgactacc tgccgcgcga
    16921 tccgttcatc gcgatggccc gcggtgaagc ccaccgggtt ccggtcatcg tcggccacaa
    16981 cgccgaagag tcgcggctgt tcaccaggtg gctgaagctg ctgcccacca ccgagcacat
    17041 gctcgagcgg ctgctcgccg gcatcgatcc cgcgtcccgc gaacgcattc agatcgccta
    17101 ccccggctat ccgcaccccg acgagtgcat gcggctgtcc gccgacatgt tcttcgcgtc
    17161 ggcggcgtgg cgtatcgccg aggcgcacag caagcacgcg ccgacctaca tgtaccgcta
    17221 cgactacgcg ccgcgcacgc tgacgtggag cggactgggc gccacccacg cgatggagct
    17281 gctcgcggtg ttcgacatct accgcacccg gctggggtcg ctgctgacgg tggccgccga
    17341 tcagcggtcg gcgctacggg tgagcaacaa catccagtcg cgctggcgcc gcttcagcag
    17401 gaccggtgtg cccgggacgg gctggcccac ctacgaccac gaccgcgcgg tcctggtgtt
    17461 cgacaagcac tcgcgggtcg aatacgaccc gcatccggcc cgccgcgagg cgtgggagaa
    17521 cttcagcctg gtgcgctgac cgtaaggtca gcgtcatggc cgacaccgcg ccccaggcac
    17581 ccttcggcaa ctaccagtac gaggtctacc tcaaggggat ggccggccag gcgccggact
    17641 tcccgatgag ctggacggag ctcgaagtgc aggcgtccgc ggcgatggac gccggcgcca
    17701 acggctacgt gttcggcagc gcgggcaccg aggacaccga gcgggagaac agggccgcct
    17761 tcacacggca ccggatcgtg ccgcggatgc tgcgcgacgt gtcgacgcgc gaccttcgca
    17821 ccgagatgtt cggagtccag cacgcggcgc cggtgctgtt ggcgcccgtc ggcgtgcagt
    17881 cgatcgtgca tcccgacgga gagctcgccg ccgcgcgcgg tgccgccgag gcgggcatcg
    17941 gattcgtcgc gtcgacggcg tcgtcgtatt cgatggagga catcgcggcg gccggcggcg
    18001 acgtgccgcg ctggtatcag ctgtactggc ccaaggaccc cgacctcacc gcaagctttg
    18061 tcgcacgcgc cgaggcggcc ggctacggag cactcgtcgt gacgctggac accggcctgc
    18121 tggcgtggcg tccgcgagac ttgcagaccg cttacctgcc tttcctgcag tcggtgggta
    18181 ttgcaaacta cttgtccgac ccggtttttc gcgccgcgct gcagaagtcc cccgaggacg
    18241 acccgacggc cgcggttctg caattcgtcg gcgtgttcag caatccggcc atgggctggg
    18301 gtgacttggc tgcgctgcgg gagctgaccg acatgccgat cgtgctgaaa ggcatcctgc
    18361 attccgacga tgcgcggctg gcccgcgacc acggcgtgca gggcgtggtg gtgtcaaacc
    18421 acggcggcag gcaggtcgac ggcgcgatcg cggcgctcga cgcgctgccc gacgtggtcg
    18481 acgccgtggg cgacgagctc accgtgctgt tcgacagcgg gatacggtcg ggcgccgacg
    18541 tcgtcaaggc gctcgcgctg ggcgcgcgcg ccgtgctgtt gggccgcccg tatctgtggg
    18601 ggatggcact gcgcggtgcc gacggcgtcg cccaggtggt gcgcaacctg ctgtcggaac
    18661 tcgatctgac actggcactt tcgggacacg cgtcaccccg cacgctcaat cgggaggtgt
    18721 tgcgcaaccg aggcgcttag ggtctagctc atgcgagccg ccgatttgag cgccgagtgg
    18781 ttgagcgctc agctgaactt gccggtgtcg tcgttcgaga cggaccgcat cggcaccggc
    18841 cagatgagcg agtgctatcg agtcgtgcct cactacggcg gcgacactat ggggccatcg
    18901 tcggtcgtgt tgaaggtcgc cgcgtcggat ccggcgagcc gtcaaaccgg tttcgcgctt
    18961 ggcctttacg aacgcgaggt gaggttctac gccgaagtgg ctccgcgact cgtcggaccg
    19021 atcgcgccct gttatcacgc ctcgttcgac gcggacgcgg ggactttcgc gctgcttctc
    19081 ggcgacgcgg ggcccgcgga ggtcggcgac gagatcgagg gcacctcggt ggagcgtgcc
    19141 cggctggcgg tctccgaact gggccgcctg cacggatccg ccatcgctga cccgacattg
    19201 gccggcgcgg cgtggctcaa ccgcgagtcg ccggtcagcc aagcattgct gacccagctc
    19261 tacgccggat tcactgagcg gtatgcagat cggatcgcgg cgcg
//
LOCUS       DASWBF010000081         9123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_927938,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000081 DASWBF010000000
VERSION     DASWBF010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927938"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VF874_02470"
     CDS             <1..862
                     /locus_tag="VF874_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9174988.1"
                     /translation="AALRSGLQQAAPTADQLNAVFSNVQDSLPQTLANLEIVIDMLKR
                     YHKGVEQAFVYFPESAAQLEAGTIFPGYGIINFALLTNLNQPPPCLTGFLPPSEWRAP
                     ADTSTQPLPSGLYCKIPKDYQGLAVRGARNYPCVDVPGKRASSPRECRSDEPYVPLGT
                     NPWYGDPNQILTCPAPAARCDQPVKPGYVIPGPSVNNGMNPLPASMLPPSGSPAPVSD
                     PLSPPGQGSVVCSGQQPNPCDYTPSQTPAAIYNPQSGEVVAPDGSRYSVRDSAGTGDD
                     GWKAMLAPAG"
     gene            902..1822
                     /locus_tag="VF874_02475"
     CDS             902..1822
                     /locus_tag="VF874_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9174989.1"
                     /translation="MRLKLGRPFLTEFTKRFDVPPADAGTPLMVTWLGVATLLISDGT
                     SALMTDGFFSRPSLATVALRRVEPSLERIIGCLERTRVRSLEAVIPVHTHYDHALDSA
                     VVAGRTEAKLVGGESAANIGRGFGQPEDRIVVATSGESMTLGAYEVTLIESKHSPPDR
                     YPGVITEPVVPPAKASAYKCGEAWSVLIHHQQTDSRLLIQGSAGFVEGALAGQQADVV
                     YLGVGQLGLLPEQYMVDYWNEVVRSVGARRVVLIHWDDFFRPLTEPLRALPYAADDLD
                     ATMRVLSRLAAEDGIPLHLPKVWEPENPWA"
     gene            1848..3086
                     /locus_tag="VF874_02480"
     CDS             1848..3086
                     /locus_tag="VF874_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX9174990.1"
                     /translation="MIVSLALLAVVLAFALVRPKGWTEDIAALPAATAVIVIGALPLR
                     DAVDEVTRLLPVVGFLAAVLVLAQLCAEEGVFQAAGGMMARGSAGRPKRLLRQVFIIA
                     SVTTAVLSLDATVVLLTPVVLATARTLRLPDRPHTYASAHLANTASLLLPVSNLTNLL
                     AFASAGLSFTRFTALMAAPWLLAIAAEYVVFRWFFAGELSTDADDDDVPATPQLPVFA
                     LVVLALTLAGFAVTSLVGLSPAWAACAGAAVLAVRGMVRRRGALGGIVRAVNLPFLAF
                     VLTLSVVVRSAMVNGLSSATRDLLPSGNSLAALLALAALAAVLSNLVNNLPAVLLLVP
                     LVAGGGAGSVLAVLIGTNVGANLTYIGSLANLLWRNVLHDNDSNDGVGRFTRLGLCTV
                     PLALVLAVVALWAGLRLIGP"
     gene            3275..4096
                     /locus_tag="VF874_02485"
     CDS             3275..4096
                     /locus_tag="VF874_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9174991.1"
                     /translation="MADPIHFVDANGLKFAYLEEGSGPLVLMLHGFPDTAHTWDDLRP
                     RIAARGYRAVSPFMRGYHPSAIPDRDPDQETLARDPLRLIEALGARDAIIIGHDWGAS
                     AAYGAAALGPDRVKKLFVIGIPHPAALKPSLKKLWGVRHFAAYKLPGAPNRFARNDFA
                     ALPAIYRRWSPAWNPDPREFDAVRASFSRPASLNAAFGYYRKLSPVPSASLKARITVP
                     VVVFAGLDDPIVEPSDYRTAARMFENEYIVEEVRGGHFMHREHPEAFAERLLTHL"
     gene            complement(4154..4558)
                     /locus_tag="VF874_02490"
     CDS             complement(4154..4558)
                     /locus_tag="VF874_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9174992.1"
                     /translation="MIGYLDTSAALRVLTIGPRPGAARLTRRTQRYLDQLVEQGHTVV
                     SSVLLRTEILCQANRQPEIDTNEVEEFLSMVNLVALDASDLDEAPNTPGELRTLDAIH
                     LRVALRIGADVMVTYDDELTRAAQAIGMGTGP"
     gene            complement(4555..4818)
                     /locus_tag="VF874_02495"
     CDS             complement(4555..4818)
                     /locus_tag="VF874_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9174993.1"
                     /translation="MRRIPHRELRNNSSQILDDVKNGEVIEVTNHGEVVAVLIPPAAT
                     PYERLVAAGKVHVARPDAKTFSRIRARRTDRDALEVLSEMRNG"
     gene            complement(4912..5850)
                     /gene="rlmB"
                     /locus_tag="VF874_02500"
     CDS             complement(4912..5850)
                     /gene="rlmB"
                     /locus_tag="VF874_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX9174994.1"
                     /translation="MAGNSRRRGAVRKPGTKKGPTVGSGGVRRRGLEGRGATPPAHMR
                     PNHPAAKRAAAKDKSRRGAKKTDETEIVLGRNPVLECLRASVPATALYVALGADADER
                     LTESVRRAADSGIPILEVPRADLDRISANGLHQGIALQVPPYEYTHPDDLLAAARDDS
                     TPALLVALDNISDPRNLGAIVRSVAAFAGHGVLIPQRRSASVTAVAWRTSAGAAARVP
                     VARATNLNRTLRDWAVAGLQVVGLDADGDTTIDELDATGDVVIVVGSEGKGLSRLVRE
                     NCDAVVSIPMAGPTESLNAAVAAGVVLAEVARQRRR"
     gene            complement(5851..7242)
                     /gene="cysS"
                     /locus_tag="VF874_02505"
     CDS             complement(5851..7242)
                     /gene="cysS"
                     /locus_tag="VF874_02505"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9174995.1"
                     /translation="MRLYDTYAGAVRDFVPLRDGHVSIYLCGATVQGLPHIGHVRSAV
                     AFDVLRRWLMARGFDVAFIRNVTDIDDKILNKAADAGRPWWEWAATHDRAFTAAYDAL
                     DVLPPSAEPRATGHITQIVELIERLIDNGHAYTGAGDVYFDVLSFADYGKLSGHKIDD
                     VHQGEGVGAGKRDQRDFTLWKGAKPGEPSWPTPWGRGRPGWHSECVAMAHEYLGSEFD
                     IHCGGMDLVFPHHENEIAQALATGDRFARFWLHNGWVTMGGEKMSKSLGNVLSIPAVL
                     QRVRPVELRYYLGSAHYRSMLEFSETALQDAVKAYTGIEDFLHRVRTRVGAIVPDGWT
                     PRFAAALDDDLSVPIALAEIHSSRAEGNRALEAGDHETALARASEIRSMMAVLGCDPL
                     DERWETRDETSAALAAVDVLVHAELERREAARAQRDWALADEIRDRLKKAGIEVTDTA
                     DGPQWSLIDGDKK"
     gene            complement(7319..7795)
                     /gene="ispF"
                     /locus_tag="VF874_02510"
     CDS             complement(7319..7795)
                     /gene="ispF"
                     /locus_tag="VF874_02510"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX9174996.1"
                     /translation="MTDLRVGLGTDVHPIEQGRPCWLLGLLFEDADGCSGHSDGDVAA
                     HALCDALLSAAGLGDVGEVFGVDDPRWDGVSGDTMLRHVRDLVEANGFRVLNAAVQVV
                     GNRPKIAPRRELAQRVLSDLLGAPVSVAATTTDGLGLTGRGEGLAAIATALVVGDE"
     gene            complement(7792..8451)
                     /gene="ispD"
                     /locus_tag="VF874_02515"
     CDS             complement(7792..8451)
                     /gene="ispD"
                     /locus_tag="VF874_02515"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX9174997.1"
                     /translation="MSAVAVVPAAGSGERLGAGRPKAFVHLGGHTLLERTLGGLRDSG
                     AVDAIVVAVPPDRTDEAKLILGADATVVAGGSHRTESVRRALAAVGNPELVLVHDAAR
                     ALTPPAMIARVVAALREGHQAVVPGLPVTDTIKVVDANGVVIATPARAGLRAVQTPQG
                     FQTELLLRAYERSKSADVTDDASMVENIGAQVHVVDGDPLAFKITTALDLLLAEAVVN
                     R"
     gene            complement(8455..8943)
                     /gene="carD"
                     /locus_tag="VF874_02520"
     CDS             complement(8455..8943)
                     /gene="carD"
                     /locus_tag="VF874_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     CarD"
                     /protein_id="HEX9174998.1"
                     /translation="MIFKVGDTVVYPHHGAALIEAIETRTIKGEQKEYLVLKVAQGDL
                     TVRVPADNAEYVGVRDVVGQEGLDKVFQVLRAPHTEEPTNWSRRYKANLEKLASGDVN
                     KVAEVVRDLWRRDQERGLSAGEKRMLAKARQILVGELALAENTDDAKAETILDEVLAA
                     AS"
BASE COUNT         1456 a         3066 c         2992 g         1609 t
ORIGIN      
        1 cgcggcgctg cgcagcgggt tgcagcaggc ggccccaacg gccgatcagc tcaacgcggt
       61 gttttcaaac gtgcaggact cgctgccgca gacgctggcc aacctcgaga tcgtcatcga
      121 catgctcaag cgctaccaca agggcgtcga gcaagcgttc gtctacttcc cggagagtgc
      181 cgcccaactc gaggccggga cgatcttccc cggctacggc atcatcaact tcgcgctgct
      241 cacgaacctc aaccagccac cgccctgttt gacgggtttc ctaccgccgt cggagtggcg
      301 ggctcccgcc gacacgtcga cgcaaccgct gccgtcgggc ctctactgca agattccgaa
      361 ggattaccag ggcctcgcgg tgcgcggcgc gcgtaactat ccgtgcgtgg acgtgccggg
      421 taagcgggcc tcgtcgccaa gggagtgccg gagcgacgag ccgtacgtgc cgctgggcac
      481 caacccctgg tacggggacc cgaaccaaat tctgacctgc ccggcacctg ccgcacgctg
      541 tgatcagccg gtcaagcctg gctacgtgat tccgggcccg agcgtcaaca acggcatgaa
      601 cccgctgccg gccagcatgt tgccgccgtc gggctctccg gcccctgtaa gcgatccgct
      661 gtctccgccg gggcagggca gcgtcgtctg cagcgggcag cagcccaacc cgtgcgacta
      721 cacgccatcg cagacccccg cggcgatcta caacccgcag agcggcgagg tggtggcgcc
      781 cgacgggtcg cggtacagcg tgcgggattc ggcaggtaca ggagacgacg gatggaaggc
      841 gatgctggcg cccgcgggct gaacccgtcg actcagtctc ggttcgccgt agggtgatcc
      901 catgcgcctc aagctcggtc gacctttcct gacggaattc accaagcggt tcgacgtgcc
      961 acccgccgac gccggcacgc cgctcatggt gacatggctg ggggtggcca cgctgctgat
     1021 cagtgacggc acctcggcgt tgatgacgga cggcttcttc tcgcggccaa gcctggccac
     1081 cgtcgcgctg aggcgggtgg aaccgtcgct ggagcgcatc atcggctgcc tcgaacgcac
     1141 ccgagtgcga tcgctcgagg ccgtgattcc ggtgcacacc cattacgacc acgcacttga
     1201 ttccgcggtg gtggcaggac gcaccgaggc caaattggtc ggaggggaat cggccgccaa
     1261 catcggccgc ggcttcgggc agcccgaaga ccgcatcgtc gtcgcgacgt ccggtgagtc
     1321 gatgacgtta ggcgcttacg aggtgacatt gatcgaatcg aaacactcgc cacccgaccg
     1381 gtatccgggc gtgatcacgg aaccggtcgt gcctccggcg aaggcgtcgg catacaagtg
     1441 cggcgaggcg tggtcggttc tgatccacca ccagcagacc gacagtcggc tcctgatcca
     1501 gggcagcgcg ggcttcgtcg aaggtgcact ggccgggcag caggcggacg tggtgtatct
     1561 cggggtcggt cagctcggac tgctgcccga gcagtacatg gtcgactact ggaacgaagt
     1621 agtgcggtcg gtcggcgctc ggcgcgtggt gctgatccac tgggacgact tcttccggcc
     1681 gctgaccgag ccgttacggg cgctgccgta tgccgccgac gatctggacg cgacgatgcg
     1741 cgtgctctcc cggctagccg cagaagacgg aatacccctg cacctgccca aggtgtggga
     1801 gcccgaaaac ccttgggcgt gacggttatt gccggtctga cgtcttgctg atcgtttcgc
     1861 tggcgctgct ggccgttgtg ctggcgttcg cgttggtgcg gccgaagggc tggactgagg
     1921 acatcgcggc tcttcccgcg gcgaccgcgg tgatcgtgat cggcgccctt ccgcttcgcg
     1981 acgcagtcga cgaggtgacg cggctcctgc cggtcgtcgg gttccttgcc gcagtgttgg
     2041 tgctggccca gctgtgcgcc gaggagggcg tgttccaggc ggccggcggg atgatggcgc
     2101 gcggcagcgc gggacggccc aaaaggctac tgcgacaggt tttcatcatc gcgtcggtca
     2161 ctacggccgt cctcagcctc gacgccaccg tcgtgctgct gactccggtc gtgctggcga
     2221 ccgcgcgcac gttgcggctg ccggacagac cgcacaccta tgccagtgct cacctggcca
     2281 acaccgcctc gctgttgctg cccgtgtcga acttgacgaa cctgctcgcg ttcgccagcg
     2341 cgggactgtc gttcacccgc ttcacggcac tgatggctgc cccgtggctg cttgcgatcg
     2401 ccgcggagta cgtcgtcttc cggtggttct ttgccggcga attatccact gacgcagacg
     2461 acgacgacgt gccggccacg ccccagctac cggtgttcgc gctggtggtg ctcgcactca
     2521 cgctggcggg cttcgcggtc acatcgctgg tcggcctctc ccccgcgtgg gccgcgtgcg
     2581 cgggtgcggc ggtgctggcg gtgcggggaa tggttcgacg gcgcggcgcg ctcggcggca
     2641 tcgtgcgggc ggtcaacctg ccgttcctcg ccttcgtgct gacgctttca gtggtggtgc
     2701 gatcggcgat ggtgaacgga ttgagttctg cgacacggga tctcctgcca tccggcaaca
     2761 gcctcgcggc gctgctggcg ctggcggcac ttgccgccgt gctgagcaat ctggtgaaca
     2821 atctgcccgc cgtcctgctg ttggtgccgc ttgtggctgg gggcggtgcg ggatcggtgc
     2881 tcgccgtgct catcggaacc aatgtcggcg ccaacctcac ctacatcggc tcgctggcga
     2941 acttgctgtg gcgaaacgta ctgcacgaca acgattccaa cgacggcgtc ggcagattca
     3001 cccgcctggg cctgtgcacg gtaccgctgg cattggtcct tgcggttgtt gcgttgtggg
     3061 ccgggctgcg actgattggc ccgtaaccga gccgacttta actttaaccg ccaaccgccg
     3121 ggatcagaac gagggcaccg ttgcagccaa taacatgcac gtacgactta ctgctattca
     3181 tcctcccact tgatcgccat cgacctctgc cggaatcggc tcagaacaga catccgcctc
     3241 cagtgcatca aggatcagcg tagtgtcccc ggctgtggcc gatccgatcc atttcgtcga
     3301 cgcgaacggg ctgaaattcg cgtatctcga ggaggggtct gggcctctcg tgctcatgct
     3361 tcacgggttt ccggacacgg cacatacgtg ggacgatctc cgcccgcgga tcgccgccag
     3421 aggataccgc gcggtcagcc cgtttatgcg cgggtaccac cctagcgcca tcccggatcg
     3481 cgaccccgat caagagactt tggcgcgcga tccccttagg ttgattgaag cgctcggcgc
     3541 tcgtgacgca atcattatcg ggcacgattg gggagcttcg gcggcatacg gcgcggcggc
     3601 gctcggcccg gatcgagtga aaaagctgtt cgttatcggc attccccatc cggcagcgct
     3661 caagccgtcg ctgaagaagc tttggggcgt ccgccatttc gcggcataca agctgccggg
     3721 ggcgccgaac cgcttcgcgc gcaatgactt cgcagcgctc cccgcgatct atcggcgatg
     3781 gtccccggcg tggaaccccg atccgaggga gttcgatgct gtccgcgcga gcttctcaag
     3841 accggcgagc ttgaacgcgg cattcggtta ttaccgaaag ctctcacctg taccgtccgc
     3901 atcactgaag gcgcggatca ctgtgccagt agtcgtcttt gcggggctcg atgatccgat
     3961 cgtcgaaccc tcggattatc gaaccgccgc gcggatgttc gagaacgagt acatcgttga
     4021 ggaggtccga ggcgggcatt tcatgcatcg ggagcatccc gaggcgttcg ccgagcggct
     4081 cttgactcat ctgtgactgc agtcagggag tccgataacc cggactccgg aacgggcgtc
     4141 aggcattgca ctctcacggt ccggtgccca tgcctatggc ctgagccgcg cgtgtcagct
     4201 catcgtcgta ggtgaccatc acatctgcac cgatacgcag ggcaaccctg agatggatcg
     4261 cgtcgagagt gcgcaactcg cccggtgtgt ttggagcctc gtcgaggtcg ctcgcatcca
     4321 gcgcaaccag gttcaccatc gacaagaact cctctacctc gttggtgtcg atttccggct
     4381 ggcggttcgc ctgacagaga atctcagttc ggaggaggac cgacgacacg accgtgtgtc
     4441 cttgctcaac cagctgatcc agataccgtt gggttctccg ggtaagacgt gcggcccctg
     4501 gacgaggtcc gattgtcaac acccgaagtg cggccgaggt gtccaggtag ccgatcaacc
     4561 gttacgcatc tccgacaaga cctcgagcgc gtcgcgatcg gtgcggcgag cccggatgcg
     4621 cgagaacgtt ttcgcgtccg gccgcgccac gtggaccttc ccggcagcga ccaaccgctc
     4681 gtacggtgtc gcggcgggtg ggatcagcac tgcaacaacc tcgccgtgat tcgtcacctc
     4741 gatgacttcg ccgttcttga catcatcgag aatctgagat gagttgttgc gcaactcgcg
     4801 atgcggaatc cttcgcatga tcctcacctt gtgccctggt ccgtagcaat agtagcaaca
     4861 ccacctgcag accgaagtgc cgtttgtgac ccacgccgag cagatgaaaa gctagcgcct
     4921 gcgttgccgg gcgacctcgg cgagcaccac cccggcggca accgcggcgt tcaaagactc
     4981 ggtcgggccg gccatcggaa tcgacaccac cgcgtcgcag ttctcgcgca ccaaacgcga
     5041 caggcccttg ccctccgagc cgacgacgat caccacgtcc ccggtggcgt ccaactcgtc
     5101 gatcgtggtg tcgccgtcgg cgtccaggcc aaccacctgc aggccggcca cggcccagtc
     5161 cctcagcgtg cgattgagat tggtcgcgcg ggccaccggc actcgggcgg cagccccggc
     5221 gctggttcgc cacgcaaccg cagtgaccga cgcgctgcgc cgctgcggaa tcagcacgcc
     5281 gtggccggcg aaggcggcca cggagcggac gatcgcaccg agattgcgag gatccgagat
     5341 gttgtcgagc gcgacgagca acgcaggcgt cgagtcgtct cgagccgccg cgagcaggtc
     5401 gtcggggtgg gtgtactcgt acggcggcac ctgcagcgca atgccctggt gcagcccgtt
     5461 ggcgctgatc cggtccaggt cggcgcgcgg cacctcgagg atcggaattc cggaatcagc
     5521 cgcacgcctg acggattcgg tcagccgctc gtcggcgtcg gcgcccaacg ccacgtacag
     5581 cgcggtagcg ggaacgctgg cgcgcagaca ctccaggacc ggattgcgtc cgagaacgat
     5641 ctctgtctcg tcagtcttct tggcgccccg gcgcgacttg tctttggcgg cagcgcgttt
     5701 cgcggccggg tggttcggcc gcatgtgggc gggcggtgtg gcgccgcgcc cctccagacc
     5761 gcgccgacgt acgccgccgg agccgacggt gggccccttc ttggtgccgg gcttgcgcac
     5821 cgcgccgcgg cgccgggagt tgcctgccat ctactttttg tcgccgtcga tcagcgacca
     5881 ctgcggcccg tcggcggtgt cggtgacctc gatgccggcc ttcttcagcc ggtcgcggat
     5941 ctcgtcggcc agggcccagt cgcgctgtgc tcgcgccgcc tcgcggcgct ccagttcggc
     6001 gtgcaccagc acgtcaaccg cggccagcgc ggccgacgtc tcgtcgcgcg tctcccagcg
     6061 ctcgtcgagc gggtcgcagc ccaaaacggc catcatcgac cggatttcgc tggcgcgggc
     6121 caatgcggtt tcgtggtcgc cggcttccag cgcacggttg ccctcggcac ggctgctgtg
     6181 tatctccgcc agcgcgatcg gcaccgagag gtcgtcgtcg agcgccgcgg cgaaccgcgg
     6241 ggtccagcca tcgggcacta tggctccgac ccgggtgcgg acgcgatgca ggaagtcctc
     6301 gatcccggtg taagccttga cggcgtcctg tagcgcggtc tcggagaatt cgagcatcga
     6361 ccggtagtgc gcactgccca ggtagtaccg cagctcgacg ggccgaaccc gttgcagcac
     6421 agcgggaatc gacagcacgt tacccagcga cttgctcatc ttctcaccgc ccatcgtgac
     6481 ccagccgttg tgcagccaga atcgggcgaa gcggtcaccg gtcgcgaggg cctgtgcgat
     6541 ctcgttctcg tggtgtggga acaccaaatc cattccaccg caatggatgt cgaactccga
     6601 tccgaggtac tcgtgggcca tcgcgacgca ttcggagtgc cagccggggc ggccgcgtcc
     6661 ccacggtgtc ggccaggacg gctcgccggg tttggcgccc ttccacaggg tgaagtcccg
     6721 ctggtcgcgt ttgccggccc cgacgccctc gccctgatgc acgtcgtcga tcttgtgacc
     6781 ggacagcttt ccgtagtctg cgaagctcag cacgtcgaag tacacgtcgc cggcgccggt
     6841 ataagcgtgg ccgttgtcga tcagccgctc gatcagctcg acgatctggg tgatgtggcc
     6901 cgtggctcgg ggttcggccg acggcggcag cacgtccagc gcgtcgtagg cggcggtgaa
     6961 cgcgcggtca tgagtggcgg cccactccca ccaaggccgt cccgcgtcgg ctgccttgtt
     7021 gaggatcttg tcgtcgatgt cggtgacgtt gcggatgaat gcgacgtcga agccgcgggc
     7081 catcagccag cgccgcagca cgtcgaatgc gacggcactg cgcacgtggc cgatgtgcgg
     7141 aaggccttgc acagtggcgc cgcacaggta gatcgagacg tggccgtcgc gcagcggcac
     7201 gaagtcgcgg acggcaccgg cgtaggtgtc gtacagccgc aagccgggag gcttgcctga
     7261 ctcgacagcc gacccgacag cttgagcgcg atcggtcacg acgtgccagc ttaccggcct
     7321 attcgtcacc aaccaccaaa gccgtcgcga tggcggccaa tccctcaccg cgcccggtca
     7381 gccctagtcc gtcggtggtt gtcgcggcca ccgacacggg cgccccgagc agatccgaca
     7441 acacgcgctg cgccagctcg cggcgcgggg cgatcttcgg ccggttaccg accacctgca
     7501 ccgcagcgtt gaggacgcga aaaccgtttg cctccaccaa gtcgcggacg tggcggagca
     7561 tggtgtcgcc gctcacgccg tcccagcgag ggtcgtcgac accgaacacc tcgccgacgt
     7621 cgccgaggcc ggccgccgac aacagcgcgt cgcacagcgc gtgggcggcc acgtcgccgt
     7681 cggaatggcc gctgcagccg tcggcgtcct cgaacagcag gcccaacagc cagcacggcc
     7741 tcccttgctc gatgggatgc acgtcggtgc ccaggcccac ccgtaaatcg gtcaccggtt
     7801 caccaccgcc tcggccagca gcaggtccag cgcggtggtg atcttgaacg ccagcgggtc
     7861 gccgtcgacg acgtgcacct gcgcgccgat gttctccacc atcgaggcgt cgtcggtgac
     7921 gtcggccgac ttcgagcgtt cgtaggcgcg cagcagcagc tcggtctgaa agccctgcgg
     7981 ggtctgcact gcccgtagtc cggcgcgggc tggcgttgcg atcacgactc cgttcgcgtc
     8041 gacgaccttg atcgtgtcgg tcacggggag gccgggcacg acggcctgat ggccttcgcg
     8101 cagcgcggcc accacgcggg cgatcatcgc aggcggcgtc agggcgcgcg cagcgtcgtg
     8161 aaccagcacc agctcggggt tgccgacagc ggccagcgcg cggcgtaccg actcggtgcg
     8221 atgggagccg ccggcgacga cagtagcgtc ggcgccgaga atgagtttcg cttcgtcggt
     8281 gcggtcaggg ggcaccgcga ccacgatggc gtcgacggca ccggagtcac gcaggccacc
     8341 gagggttcgc tccagcaggg tgtgcccgcc aaggtggacg aacgctttcg gtctgcctgc
     8401 gcccaggcgt tcgccagaac ccgcggccgg aacgaccgcg actgcgctca tcctctacga
     8461 ggcggcggcc aaaacctcgt cgaggatggt ctccgctttc gcatcgtcgg tgttctcggc
     8521 cagtgcgagc tccccgacca gaatctggcg cgccttggcc agcatccgct tctcgccagc
     8581 cgacaggcca cgttcctggt cgcggcgcca caaatcgcgc acgacctcgg ccaccttgtt
     8641 cacgtcgccg gaagccagct tctcgaggtt ggccttgtag cggcgcgacc agttggtggg
     8701 ctcttcggtg tgcggcgccc gcagcacctg gaagaccttg tcgagccctt cctggcccac
     8761 cacgtcgcgc acacccacat actccgcgtt atctgcaggt actcgaactg tcaggtcacc
     8821 ctgggcgacc ttcaagacga gatattcttt ctgctcgcct ttgatggtcc gggtttcgat
     8881 cgcttcgatc aacgcagcac cgtgatgtgg atagacaacg gtgtctccga ccttaaaaat
     8941 catctgattc gagccccttt cgccactcca tgctaacacg gtcctctgac gggcgtttaa
     9001 caacggtgca ggtcaggggc acagtgggtg aacattgggg gttgacacag cggcgaatac
     9061 atgcatgcca gcggtttcat cgacagcgcc gcaggtagct ggtcgcggtg ttgccgacga
     9121 cct
//
LOCUS       DASWBF010000082         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_267377,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000082 DASWBF010000000
VERSION     DASWBF010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267377"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..915
                     /locus_tag="VF874_02525"
     CDS             88..915
                     /locus_tag="VF874_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9174999.1"
                     /translation="MFCKALTPLANGFCFGEGARWFEGLLWFSDMLGEAVHTVNLQGS
                     MSTLPLPGHAPSGLGFRPDGSLLIASTESRQVLRYDGETVTVLADLADAAPANLGDMV
                     VDDLGRAYVGSQAREGGVIVRIDPDGTAAVVATDLDFPNGMVITPDGTTLIVAESVGR
                     RLTAFSIGPDGSLSDRRIFAEGLDGPPDGITLDADVGVWASMTLAHQFERIVEGGAVT
                     DRIDIGDRTAIACMLGGPERRTLFLLSSTDAYPQRLIGTKLSQLDAVTVDIPGAGLP"
     gene            912..>1556
                     /locus_tag="VF874_02530"
     CDS             912..>1556
                     /locus_tag="VF874_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX9175000.1"
                     /translation="MSDSYYELLDDADPLGEKFTATDLVRSTWSASIQHAAPVSSLLV
                     RGLERCARRDDTRLSRVVVDLLGPVPAEGEFWVRSTLERAGKQIELVSAEMLGLGPDG
                     QHRPVAKASGWRLQQTDTHDVVRASAPPLRPLAETHVRNMKERWDRNYVHSLDWRWLT
                     HRGDSGPGESWIRPTVDLVKGEGMTPLERLFAVADDANGIGSKIDIRTWTFLNTD"
BASE COUNT          245 a          515 c          539 g          257 t
ORIGIN      
        1 gcagcgtgtg gctgtggtac ttgcgcatcg cccttcaccg gccgcggata gttgacagtg
       61 gcacctgtcg agcgtgacac ttatggcgtg ttttgtaaag ctctgacacc actggccaac
      121 ggcttctgct tcggagaggg cgcccggtgg ttcgaggggc tgctgtggtt ttccgacatg
      181 ctcggcgagg cggtgcacac cgtcaacctg caaggatcga tgagcacgct gccgctgccc
      241 ggccacgccc cctccggtct gggctttcgg cccgacggct cgctgctgat cgcctccacc
      301 gagagccggc aggtgctgcg ctacgacggc gagaccgtca ccgtcctggc cgatctcgcc
      361 gacgccgcgc cggccaacct cggcgacatg gtcgtcgacg acctcgggcg cgcctatgtc
      421 gggtcccaag cgcgcgaggg cggtgtcatc gtgcggatcg atcccgacgg caccgcagct
      481 gtcgtcgcga ccgacctcga cttcccgaac ggaatggtca tcaccccgga cgggacgacc
      541 ttgatcgtcg ccgagtccgt cggccggcgg ttgaccgcgt tcagcatcgg ccccgacggg
      601 tcactgtccg atcgccggat cttcgccgaa ggtctcgacg ggccgcccga cggcatcaca
      661 ctcgacgccg acgttggagt ctgggcgtcg atgaccctgg cccatcagtt cgagcggatc
      721 gtcgagggtg gcgccgtgac ggaccgcatc gacataggcg accgcacggc gatcgcctgc
      781 atgctgggcg ggcccgagcg gcgaaccctc ttcctgctgt ccagcactga cgcctatccg
      841 caacgcctca tcgggacgaa gctgtctcag ctcgacgcgg tgacggtcga catcccaggg
      901 gcgggccttc catgagcgac tcctattacg agctgctcga cgacgccgac ccgttgggcg
      961 agaagttcac cgcgaccgac ttggtgcgca gcacgtggtc ggcgtcgatc cagcacgccg
     1021 ctccggtgtc gtcgctgttg gttcgcggac tggagcgctg cgcgcggcgc gacgacaccc
     1081 ggctgagccg ggtcgtcgtg gatctgctgg ggccggtgcc ggccgagggc gagttctggg
     1141 tgcggtcaac gctggagcgc gccggtaaac aaatcgagtt ggtcagcgcc gagatgctgg
     1201 gcctcggacc ggacgggcag catagacccg tcgccaaggc cagcggctgg cggttacagc
     1261 agacggacac acacgacgtc gtgcgggcgt cggcgccgcc gctgcgaccg ttagcggaaa
     1321 cccacgtccg caatatgaag gagcgctggg accgcaatta cgtgcacagt ttggactggc
     1381 ggtggctcac ccatcggggc gacagcggcc ccggcgagtc gtggatcagg ccgacggtgg
     1441 atctggtcaa gggcgagggc atgacgccgt tggagcgcct gttcgcggtg gccgacgacg
     1501 cgaacggcat cggctccaag atcgacatca ggacgtggac gtttctgaac accgac
//
LOCUS       DASWBF010000083         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1056872,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000083 DASWBF010000000
VERSION     DASWBF010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056872"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..156
                     /locus_tag="VF874_02535"
                     /pseudo
     CDS             1..156
                     /locus_tag="VF874_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
     gene            431..1012
                     /locus_tag="VF874_02540"
     CDS             431..1012
                     /locus_tag="VF874_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9175001.1"
                     /translation="MAELGYARVSTTHQDLERQLDALAAQGIPPERTWQDKKTGATIN
                     RPGFAELRHYARPGDTIVATNLDRIGRNLRECLNLVHELREQGVGIKTLRDPIPIDTS
                     DDSPLAQIAVAMLALFAEMERTFARERAAHARAVALNKGKLPGRPRKLNDHKLQAARA
                     SLDAGMHLDDLAEMLNIHRATLYRALQRDEREQ"
     gene            complement(1039..1266)
                     /locus_tag="VF874_02545"
     CDS             complement(1039..1266)
                     /locus_tag="VF874_02545"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX9175002.1"
                     /translation="MPGLQVLGVGGLPEIGPGDDIGAMVHAAASAQGDPLADGDVVVV
                     TSKIVSKSEGCAVELAEVTPSEFGPGLPKVT"
     gene            complement(1277..2062)
                     /locus_tag="VF874_02550"
     CDS             complement(1277..2062)
                     /locus_tag="VF874_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175003.1"
                     /translation="MPHAAVNGQRLYFEDTGGSGPPVVFSHGFLMDHEMFAPQVEALR
                     DAYRVITWDERGFGLTEYDDQPFSYWDSARDCLGLLDHLGVELAVLGGMSQGGFLSLR
                     AALLSPQRVRALILLDTQAGTEDPVAVEGYTQMKDTWLAVGPVPELAGPIADLIIADP
                     EHNEVWIAKWQARDKGFLAVPFECLLSRDDITDRLGEIRCPALVVHGTEDTAITMDQT
                     EELAAGLPGAGAVVAVGGAHAANLTNPVPVNAAIVEFLGALPD"
     gene            2111..2293
                     /locus_tag="VF874_02555"
                     /pseudo
     CDS             2111..2293
                     /locus_tag="VF874_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          471 a          972 c          835 g          399 t
ORIGIN      
        1 cgcctacacc cttacaccgg gctgatcggc accagcgtct ccggggtgtt cctgtatctg
       61 atcgcgatca tcaacatcat catcatcctg ggcggaatcc tgcgggtatt ccgggacatg
      121 cgcacggggc actacgacga agccgcactc gacgaggtcg gcgtcgtcta cccctgacca
      181 gcggcaccga accgggaacc gaatctgttc tgtgacaagc gaccgcgacc gagcgccacc
      241 gctggccgcg ctctggtaat ccggtcgcga aaccacagtt tcgcgaccgc gcccgccaga
      301 tcactcctgc cgacgcccac aacgcaaaat gctgtcgcac attccagtcg caaaatcttt
      361 ctccgcataa cctgtgcgcg ataagattct gcgacaccac ccgggttggt gtcgcagaag
      421 ggaaactccg gtggccgaac tcggctacgc acgggtatcc accacccacc aagacctcga
      481 acgtcaactc gacgcgcttg ccgcacaagg catcccgccc gaacggacct ggcaggacaa
      541 aaagaccggc gctaccataa accgtcccgg cttcgccgag ctgcgccact acgcccgccc
      601 cggcgacacc atcgtggcca ccaacctcga ccgcatcggg cgcaacctgc gcgaatgcct
      661 gaacctggtg cacgaactgc gcgagcaagg cgtgggcatc aagaccctgc gtgatccgat
      721 ccccatcgac accagcgacg actcgccgtt ggcccagatc gccgtagcca tgctcgcact
      781 gttcgcagag atggaacgca ccttcgcccg cgaacgcgcc gcacacgcac gggccgtcgc
      841 cctcaacaaa ggcaagcttc ccggccgccc ccgcaagctc aacgaccaca aactccaggc
      901 cgcccgcgcc tcactcgacg ccggcatgca cctcgacgac ctcgccgaga tgctcaacat
      961 ccaccgcgcc accctctacc gagcactcca acgagacgag cgcgaacaat agaccggccc
     1021 ggttgcttac cttgcggctc aggtcacctt cgggagtccc gggccgaact cggacggcgt
     1081 cacctcggcg agctcgaccg cgcagccctc ggacttcgac acgatcttcg aggtgacgac
     1141 cacgacgtcg ccgtcggcga gcgggtcgcc ctgtgccgac gccgccgcgt ggaccatcgc
     1201 cccgatgtcg tctccggggc cgatctcggg cagcccgccg acgccgagca cctgcaggcc
     1261 gggcacgacg ggcgtctcag tccggcaggg cgccgaggaa ctcgacgatc gccgcgttca
     1321 cgggcaccgg gttggtcagg ttggcggcgt gcgcgccgcc gacggcgacg accgcaccgg
     1381 cgcctggcag gccggccgcc agctcctcgg tctggtccat cgtgatcgcg gtgtcctcgg
     1441 tgccgtggac caccagcgcc gggcagcgga tctcgccgag ccggtcggtg atgtcgtcgc
     1501 gcgacaagag gcactcgaac ggcacggcga ggaacccctt gtcgcgcgcc tgccacttgg
     1561 cgatccagac ctcgttgtgc tcggggtcgg cgatgatcag atcggcgatc ggcccggcga
     1621 gctcgggcac cggaccgacg gcgagccagg tgtccttcat ctgcgtgtac ccctccaccg
     1681 ccaccggatc ctcggtcccc gcctgcgtgt cgagcaggat cagagcgcgc acccgctgcg
     1741 gtgacagcaa cgcggcgcgc agcgagagga agccgccctg gctcatgccc ccgagcaccg
     1801 ccagctcgac gccgaggtgg tcgagcagac cgaggcagtc gcgggccgag tcccagtagc
     1861 tgaacggctg gtcgtcgtac tcggtgagcc cgaacccgcg ctcgtcccac gtgatcacgc
     1921 ggtacgcgtc gcgcagcgcc tcgacctgtg gcgcgaacat ctcgtggtcc atcaggaagc
     1981 cgtgggagaa cacgaccggc ggacccgacc cgccggtgtc ctcgaagtag aggcgctgac
     2041 cgttgacggc ggcatgcggc atggggcgac cgtacccgac gtccgagcgc gctcaggcca
     2101 acatgggcgg atgagcgacg atgcgaacca gcgtgcaggc ggacaccggc cggtgtcgga
     2161 gatcttcgat cccgcggcct ggcggaccgt cgacgggttc gacttctccg acatcaccta
     2221 ccaccgcgcc cgcgacgccg gcgtcgtgcg gatcgccttc gaccggccgg aggtgcgcaa
     2281 cgccttccgc ccggggtcgc cgctggtgcg tacgcagggt ctcccacgcg tcgtccttgt
     2341 cgccttccgg cgtcgttcgt cagcgcctac ttcgcggtgc gtttcaggcc acctcagtgc
     2401 gcatgtacgg atcgggtgcc tgcaaggcca ggtgccagtt ctgtttgaga cggttggaac
     2461 gaaaaggaaa accgtcgtgc acgcgtaacg cacagcgccg cagcgtctta cgaaaagagt
     2521 gccgcgggtg cggatcgatc ccagtgcgcg gctacgcagc tagcgcgcta tcgttgcagc
     2581 aatagttgcc cggttatcgg gcatttatcg gccgtacccc gggagcgcca gacctcgggc
     2641 agccgtccat ccagtgcgct ggagagtctg ttggatt
//
LOCUS       DASWBF010000084        15929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_991001,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000084 DASWBF010000000
VERSION     DASWBF010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15929
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991001"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..360)
                     /locus_tag="VF874_02560"
                     /pseudo
     CDS             complement(268..360)
                     /locus_tag="VF874_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(421..822)
                     /locus_tag="VF874_02565"
     CDS             complement(421..822)
                     /locus_tag="VF874_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HEX9175004.1"
                     /translation="MDSSAVWRLQRQPELTEAWNSSLLSGAVGSCEPQRAEFRRSARN
                     AYDFDQMNQTFRDVYPDVPVPKSVWRWIDSAQRRLAGAGAVRALSVVDLLICGTAAAR
                     GLVVLHDDADYELAQRHLADVSAWRVVSADQ"
     gene            complement(903..1055)
                     /locus_tag="VF874_02570"
                     /pseudo
     CDS             complement(903..1055)
                     /locus_tag="VF874_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
     gene            complement(1297..2253)
                     /locus_tag="VF874_02575"
     CDS             complement(1297..2253)
                     /locus_tag="VF874_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175005.1"
                     /translation="MGVNQMLVLNRADLESLGLTWAEIIDVLDDAFVQKARGLVQNPP
                     KPKVTSRDDAFIHAMPAYLGGSDRIGIKWVAGYEQNHAKGLPYIYGAVIMNDAETGRP
                     IALLDGGWITEMRTPGVSGVTMRHVPGDPKHLAIVGGGVQGRRHLEVALQVHAGLTHV
                     TAYDYVDGVAQQLLDNAGDRVTTVAETPIDAVAGADLVITTITVPLEPKLDCAYTDPN
                     ALLLPVDYDDAMAPAAFHDAVVYCVDDLGQYGSVAESLYFFGLPKPDTHLAEVVAGKY
                     NVPVTGRRMFLNMGIAMDDVALGSLALDRATATGAGRYIEFP"
     gene            complement(2387..3973)
                     /locus_tag="VF874_02580"
     CDS             complement(2387..3973)
                     /locus_tag="VF874_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEX9175006.1"
                     /translation="MGDTTKPQVASAKSGLRIPGFAQLQRLGKSLMLPIAVLPAAGIL
                     LRLGQDDLLGKIHAPVIGPFFQAMSAAGGALFTNLPLLFAVGVAIGFARKADGSTALA
                     AAVGYLVVQAVFKTMSPIVLAGEVDKAGDQAQINYSVFAGIVVGLVTAWLFDRYHTIQ
                     LPSYIGFFGGRRFVPIVVSLACLVIAFAMSYFYPVFDAGLTGLGKFIGGSGALGAFVY
                     GFANRMLIPLGLHHIPNSYVWFIYGDYQSPDGTVATGELSRFAAGDPSAGILTSGFYP
                     ILMFGLPAAALAMIHVANKRQRKLAVGILSAAALTAFLTGVTEPLEFAFMFVAFPLYL
                     IHAVLTGLSLAIAYLLDIHLGFSFSAGLIDLLLYCTAPAAKNIPLLIGMGVVFFAVYY
                     FLFRFAITKWNLRTAGREPETEFEAEERANLGDGDDAVTSVEARGSGAATTMTAPAPV
                     AADTEAEQVIAAFGGRDNLVNVDACITRLRVEVADKSKVDQARLRSLGAAGVVEVGNS
                     VQAVFGTQAEALKNDIKDIL"
     gene            4078..4548
                     /locus_tag="VF874_02585"
     CDS             4078..4548
                     /locus_tag="VF874_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HEX9175007.1"
                     /translation="MSNTPVLAPIEGRAVPLDDVPDPVFSQGMVGYGAAIDPPRGVID
                     AVAPVCGKILKLLPHAYIVMTPENVGILVHLGLDTVQLNGEGFTTHVSQGDTVTAGQV
                     VVTYDVPSVEAKGLNPVVPVVVMDEREPGNVIPSEIVTARAKIACGATLFTANK"
     gene            4549..5334
                     /gene="nagB"
                     /locus_tag="VF874_02590"
     CDS             4549..5334
                     /gene="nagB"
                     /locus_tag="VF874_02590"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX9175008.1"
                     /translation="MEVIILQDAKEIGGIAADSIGALLRRKPTAVLGLATGSSPLAIY
                     DELVSRCDAGLISFRLARGFTLDEYVGLPADHPQRYRNVIVNVLVSRVDFAPGAVQGP
                     DGLAADIPTACAEYENAIQHAGGVDLQILGIGTDGHIGFNEPGSSLGSRTRIKTLTRQ
                     TRLDNARFFDGVVDSVPTHCLTQGLATIMAARHLILVAIGRRKSEAVHHLVEGPVSAL
                     WPATILQHHPHVTALLDDAAAERLQLANYYRETYRSKPDRQGI"
     gene            5344..6504
                     /gene="nagA"
                     /locus_tag="VF874_02595"
     CDS             5344..6504
                     /gene="nagA"
                     /locus_tag="VF874_02595"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX9175009.1"
                     /translation="MLMTADTLLTGSELLRPAWIEIERGTVHAIGSGAPPRLADRNLG
                     AVTVVPGFVDTHVHGGGGANFSTASPADTATAAGLHVRHGTTMLVASLVTASPVDLIR
                     QVGELAEDVRRGVIAGIHLEGPWLAAERCGAHEPSLMRDPDPEELDRVLAVGGAAIRM
                     VTLAPERVGALAAVRRIVDAGVVAAVGHTDATYEQTRAAIAAGATVGTHLFNAMRPIN
                     HREPGPVIALLEDSRVTVELITDGVHVDPALYRHVCRSVEPDRVSLVTDAMAAAGMAE
                     GRYRLGPVAVDVVDGVARVAGTQTIAGSTATMDRQFRYAVTDSGLPGDEALMAAVRQA
                     SINPARALGLPYAGLAPGASADLVVLDADLAVTGVLRQGLWVIEPETVSSTR"
     gene            6577..6945
                     /locus_tag="VF874_02600"
     CDS             6577..6945
                     /locus_tag="VF874_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX9175010.1"
                     /translation="MIEKLSDMPEGVTGISVSGRLRGDDLREFKPAMEELLKTGEIRI
                     VEVIAPDYEGFGPGGMIEDLKLGLGALIQHHSAFKRIAVVSDKDWVAHALHAFAWMVP
                     GELALFGLDELDRAKQWAAG"
     gene            7211..7630
                     /locus_tag="VF874_02605"
     CDS             7211..7630
                     /locus_tag="VF874_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9175011.1"
                     /translation="MPKQLHHVVMCSHSADALVRFLTDVVGMEVQHQFRVPGEVLEKT
                     LGWPPSDGADLTMLGSGVAGLIEVLDVPEHLRATAPEGLTALSFLTDDVRNVQDKARD
                     YASDVTVLDAGVPGVDLFFCTVGGVPMEFMGSYEAER"
     gene            complement(7627..7992)
                     /locus_tag="VF874_02610"
     CDS             complement(7627..7992)
                     /locus_tag="VF874_02610"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEX9175012.1"
                     /translation="MNEPRKQPVYKVLLFLKRRPGMSVEAFRDYYENVHSKIGEKYSQ
                     GLLRYVRRYVDPVGGEELPFDVITELWLADRATAEAIATHTANNEPPPEVLEDEKRLF
                     DRSKSRVATVVEFESALAD"
     gene            complement(7989..8969)
                     /locus_tag="VF874_02615"
     CDS             complement(7989..8969)
                     /locus_tag="VF874_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9175013.1"
                     /translation="MTIELSCGLPPGPAFSDLSVLAEDVGYSRVWIFDSAPLWEDPFV
                     HLALAAERTSRIGLGTAVLIPTERSEMAMASSIAAIARLSGGRFRACFGTGGTARRTM
                     GQRPLTLRVLGEYVSAVRGLLAGETVTIQGAPVRMLHADGLTASRPVDVEVWLSAFGP
                     RATELAARIADGIIGLPVRQSLPVATMRAGTVLEPGEDRDSPRVREAVGPWQVVAFHD
                     AYAVAGAAAVDAMPGGREWREALERLSAEGGRHLLVYEGHVTHLTERDYPLVGYTGGF
                     PTMIGEPDKIRASLAQLEDRGVQELIYTPSGPDVARELRAFCAAAQGNSR"
     gene            complement(9129..10280)
                     /locus_tag="VF874_02620"
     CDS             complement(9129..10280)
                     /locus_tag="VF874_02620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9175014.1"
                     /translation="MAEFTFTEEQAQLRDAVRRFCAENFDEQTVRRLMESDPPFDPKV
                     WARLGSELGVLGLSVPESGGGAGGTLVDQALAVEQLAASLACGPVFGTVFLAVPALVA
                     ASAGPVREELLTELTEGRRTAAFAVADWAGAFDPAAVTVTADGDALTGAVERVVDGGA
                     ADDLLVAADGPDGLGLYAVAAAGPGVERIPLVTLDLTRPQATIRFSDAPARLVAGADE
                     AERVIGHALQVGSALLAVEQVGASQHLLDLSVDYAKSRLQFGRPIGSFQAVKHRLADL
                     LVELEHARSTAYHAVWALTDGTDDPALAASIAQATCSAAFSRIAADTIQVHGGIGFTW
                     EHQAHLYLKRATTDAALLGTAEQHRSRVAALVLDTAEAGRVPPVATGAS"
     gene            complement(10273..11469)
                     /locus_tag="VF874_02625"
     CDS             complement(10273..11469)
                     /locus_tag="VF874_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9175015.1"
                     /translation="MSTVADADRVADLARHVVRDHDPKSVPIPEFLGACFDAGLSWVH
                     FPEGLGGLGVSRGLQAVADRILQGAGGPVPLALNPMGYGMAAPTVREHARSDEVKQRL
                     LRPLATTEDIWCQLFSEPGAGSDLAGLATSAVPDGDEWVVNGQKVWTSLAHRARWGLL
                     LARTNPDMPKHKGLTYFVLDMHAPGVETRPLRQLTGHAEFNEVYMTDARIPDAYRLGE
                     VGDGWRVAMTTLMNERSALGASGSRRGAGTIADAVSLWASRPDLQTPVLRDRLTQLWL
                     RAEAQRLTSERSRAAATVGGPGPEGSIGKLVGAELNQYIYQFCMDFLGREGILYHSYA
                     MRDSDQDVEDWRGPVQQRFLRSRANTIEGGTSEVMRNILGERVLGLPGDLRADAGMPW
                     KEIPRG"
     gene            11541..12833
                     /locus_tag="VF874_02630"
     CDS             11541..12833
                     /locus_tag="VF874_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9175016.1"
                     /translation="MAREDRDRLSVDDARILGLESATITGHTLKLIVLEPGTGPLDLV
                     ALQAEVAKRLPTQPRATQRVDVSGNEPRWVDATDFDIREHVRRQAKSECASRDDLWRA
                     VSALMSEHLDRERPLWTFDLIGPLPDGREAIAVRIHHAMADGIAAVRFLEAVLWDPHL
                     DEPAGERAQPGLRSAPAPSWLAEAWRMPGAVRRELGHPGSRSPFDRPIGGSRALAFTV
                     ASLAELKAIGASRPAHTTVNDVLLTIVAGGLRAWLAAGEAATPHLHAQVPVSLHHRDE
                     GADDLGNRDSFINVDLPLAEADPLVRLDLISAETSKRKRLGDAEELYDLFHALGRMKY
                     VGSAVQRLAKSAREFSVAISNVPGPSAPVSVVRRRVQHLFSSSEPATHHALRISAISC
                     AGDIGIGLCTDPQALPDIARLAEAIERSYAELRSAAIG"
     gene            12817..13956
                     /locus_tag="VF874_02635"
     CDS             12817..13956
                     /locus_tag="VF874_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9175017.1"
                     /translation="MRQSDNRKRVVVAGLGDTGVLTAIHLSRHADVVGISVKPALVSG
                     QELGVRLSRPDDWARDYWIAFDRFRRLDRVRTVHAALAGVDFAARKVFGQRADGFALA
                     EGYDALVIATGVANGFWRRPTLQSQGEIGAELRAAHDRLAAAKSVIVVGGGAAAVSSA
                     ANLAMTWPATRVGLYFPGERALVRHHPRTWERIRRRLTEIGVVLHPGHRAVVLQGFAC
                     DQITSEPVQWSTGQPSTSADAVLWATGRVRPNTDWLPAELLDDDNFVRVTPELRVPHH
                     AGVFAVGDVAATDPLRSSARNRADRSVAHNVLAEFGGRPMRTFRAPQRRWGSVLGIQP
                     DGLEVFSPTGHAFRFPAWSTDRVLMPWIVRRGIYRGVRENRITAS"
     gene            complement(13943..14758)
                     /locus_tag="VF874_02640"
     CDS             complement(13943..14758)
                     /locus_tag="VF874_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEX9175018.1"
                     /translation="MGTLDGKVAFITGAARGLGRAYAVKFATEGADIIAVDICQDFAT
                     TDYPGSTPEDLTRTEQLVQAQGRTILSRHADVRDRDAVDAVVREAVEHFGRLDIVIAN
                     AGIIRLTDSDEPDATFRDIVDVNLIGAWNTVEAAIPALVGGNRGGSIVLASSTAGLDA
                     SSTDRPGLQAYAASKRGLVALMQGWAKYLAPQSIRVNTIHPSGVATDMILNETTMTMA
                     AANDPWLADMQNALPIPLLQPEEVAGAVAWLVSDDARFITGTAWPLDAGFTMR"
     gene            complement(14761..15594)
                     /locus_tag="VF874_02645"
     CDS             complement(14761..15594)
                     /locus_tag="VF874_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX9175019.1"
                     /translation="MARWLITGCSTGIGREIAVAALQAGHDVVVTARRPETVQDIVDE
                     FGGHAVSVALDVTDKSQIAAAVEAADEAFGGIDVLVNNAGHGYLSAVEEGEDAEVRKL
                     FDINYFGVVDMIKAVLPAMRAGRSGHIVNISSMTGLVANPPNAYYSSTKFALEALTEA
                     LSAEVKPLGIKVTAIEPGAFRTDWAARSMKETSTPITDYEENVGERKTMIKEFANHLP
                     GDPRKVAEAVLMVTTLDEPPLRLLLGRDVLKAMRDKIAAMTASIDEWEAVTRDVNFRG
                     A"
     gene            complement(15596..>15929)
                     /locus_tag="VF874_02650"
     CDS             complement(15596..>15929)
                     /locus_tag="VF874_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175020.1"
                     /translation="DIVDRTGSLNKAQSVANEDGTYTYVISAVDPGVQNWIDSDGLSE
                     GILTLRMAEFGEGSPTEDLGARGRVVKLDALDSEVPHLRWVSSEERATELAHRRAGYL
                     RRLPEGTL"
BASE COUNT         2699 a         5322 c         5224 g         2684 t
ORIGIN      
        1 gctcgcaccc gcctactgga acggctgggg accctggcac ctgacaccat gcaccaagtc
       61 gagcgcggcc ttgcactgat tctgggcatc cacgattgat ggatcctggc tggcagcacc
      121 gaagacgctg ccggagctgt cacggaagcg agaacctaca actgcagaat cttacgacca
      181 acggcaggcg agttgcattg tgagacaacc cctttccctc cacggcgcgt gctactcgcg
      241 cggggcgact cgtgaacttc cggcctgtca gcatccggtg cttgcgcgga gtatgcggca
      301 ggcttcgtgt aggcgggttg gggacagtcg gctgatgcgg cgtcggaatg cgctgcgcgg
      361 cctccgacgc cgagtaggcg acgatgccgg aaagagggag cagccggcct gaggaacccg
      421 ctactggtcg gcgctaacga cacgccacgc gctgacgtca gcaaggtggc gctgtgcgag
      481 ttcgtagtcg gcgtcgtcat ggaggactac taggcccctg gcggccgcgg tgccgcagat
      541 cagcagatcg acgaccgaca gggcacgcac cgcacccgcg ccggcgagcc ggcgctgcgc
      601 cgaatcgatc caccgccaca cggacttcgg cactggcaca tcggggtaga cgtcacgaaa
      661 cgtctgattc atctgatcga agtcgtaagc attccgcgcc gatcgacgga actcggctcg
      721 ttgtggttcg cacgatccga cggccccgct gagaagcgag gagttccagg cctcggttag
      781 ttccggttgt cgttggagcc gccagaccgc ggaggaatcc accaagaagt ggatcaagtc
      841 ccgacagcct tctcgtcggc ccgcgcggcg agccagccct cgtaatccca gcccttcgcc
      901 tgctcgcgcg accgggccag cgcctcgatg cgccggaacc gctcgacgta atcgcgcatc
      961 gcgaggttca cggcttcctt cttggtgtgc actccggcga tccgcataac ttcggacagc
     1021 gcttcgtcgt caaggtcgat ctgggtcacc gacaccacgg cctcccatgt tggtgatgta
     1081 tggtgagtgc atacattagc aacataggat ggtctttact ggtcgcccgg ccagaccgat
     1141 agacgaatcc agaacttgag aagcggtgac acctcccgcg ttccaatcgt cctcgtcttc
     1201 atcagttctg ctgttcttcg gatcggattg cctggtagtg atgtagccag ggaatgagga
     1261 tgccaccggt tacgctccct gtgcctgcac gaggtctcaa gggaattcga tgtagcggcc
     1321 cgctccggtc gcggtagcgc gatcgagcgc cagcgagccg agggccacgt cgtccatggc
     1381 aatgcccatg ttgaggaaca tccgccgacc cgtgacgggc acgttgtact ttccggcgac
     1441 gacctcggcc agatgcgtgt caggcttggg gaggccgaag aagtaaaggc tctcagcaac
     1501 cgagccgtat tggccgagat cgtccacgca gtagacgacg gcgtcatgga acgcggccgg
     1561 cgccatagcg tcgtcatagt ccacaggaag cagcagtgcg ttcggatcgg tgtaggcgca
     1621 gtccagcttc ggctcgagcg ggacggtgat ggtggtgatc accaggtcgg cacccgccac
     1681 cgcgtcgatg ggtgtctccg cgaccgtcgt tactcgatcg ccggcgttgt cgagcagctg
     1741 ctgggcgacg ccgtcgacat agtcgtacgc cgtcacgtgg gtcaacccgg cgtgcacctg
     1801 cagcgcgacc tccagatggc gcctaccctg cacaccgccg cccacgatgg ccaggtgctt
     1861 cggatctccc ggcacatgcc gcatggtcac gccggagacg ccgggggtgc gcatctcggt
     1921 gatccagccg ccatcgagta gtgcgatcgg tcgcccggtc tccgcgtcgt tcatgatcac
     1981 cgcgccgtag atgtagggga ggcccttggc gtggttctgc tcgtagccgg cgacccactt
     2041 gatgccgatc cggtctgagc cgcccaggta ggccggcatc gcgtggatga acgcgtcgtc
     2101 gcgggacgtg accttgggct tgggcgggtt ctgcacgagg ccacgagcct tctggacgaa
     2161 ggcgtcatcg agcacgtcga tgatctctgc ccaagtcagg cccaggctct cgaggtcggc
     2221 acggttgagg accagcatct ggttgacgcc catggaaaga ccgtagcgcc gctgcccgcc
     2281 gcctttgacg gatcgagtgt cagagccggt caactccggt caccgtctct acgctggtcg
     2341 gcgcggcatc tgaaggagct ggccgatgag gacctgatgg ccgcgtctag aggatgtcct
     2401 tgatgtcgtt cttcaaggcc tccgcctggg tgccgaagac cgcttggaca ctgttgccga
     2461 cctcgacgac accggcggcg ccgagactgc gcagacgggc ctgatcgacc ttgctcttgt
     2521 cggcgacttc cacgcgcagg cgagtgatgc aggcgtcgac gttcacgagg ttgtcccggc
     2581 cgccgaacgc cgctatgact tgctcggcct cggtgtcggc cgcaaccggg gcgggtgcgg
     2641 tcatcgtcgt cgcggcgccg gatccgcgcg cttcgacaga cgtgacggcg tcgtcaccgt
     2701 cgcccaggtt ggcgcgctcc tccgcctcga attcggtttc cggctcgcgt cccgcggttc
     2761 tcaagttcca cttcgtgatc gcaaaccgga acaagaagta gtagacggcg aagaacacca
     2821 cgcccatgcc gatgagcagc gggatgttct tcgcggcggg tgccgtgcaa tagagcagca
     2881 ggtcaatcag cccggcagag aacgagaatc ccaggtggat gtcgagcagg tacgcgatgg
     2941 ccagggatag cccggtgagc actgcgtgga tcaagtacaa cgggaaagcc acgaacatga
     3001 acgcgaactc gagcggctcg gtaaccccgg tcaagaacgc ggtcagggcc gccgccgaca
     3061 gaatgccgac agcgagcttt ctttggcgtt tgttcgccac gtggatcatg gccaaggcgg
     3121 cagccggcag cccgaacatc agaatcggat agaaccccga tgtcaggatt ccggcggaag
     3181 ggtcgccggc ggcgaaccga ctgagttcgc cggtggcgac ggtcccgtcg gggctctggt
     3241 agtccccgta gatgaaccag acgtacgagt tcgggatgtg gtggaggccg agcggaatga
     3301 gcatccggtt ggcgaacccg tagacgaacg cgcccaaggc tcccgagccg ccgatgaatt
     3361 tgccgaggcc ggtcagcccc gcgtcgaaga ccggatagaa gtagctcatg gcgaatgcga
     3421 tgaccaggca ggccagtgac accacgatcg gaacgaaccg ccgccccccg aaaaagccga
     3481 tgtaggacgg caattggatc gtgtgatagc ggtcaaacag ccacgcggtg accagaccga
     3541 ccacgattcc cgcgaacacg ctgtagttga tctgggcttg gtcgccggcc ttgtctacct
     3601 ccccggcgag aacgatcggc gacatggtct tgaacaccgc ttgcaccacc aggtagccca
     3661 ccgcggcggc gagcgcggtg gacccgtccg cctttctggc gaatccaatg gcgacaccga
     3721 ccgcgaacag cagagggagg ttggtgaaga gggcgccacc ggcagcgctc attgcctgga
     3781 agaacggtcc gattaccggc gcgtggatct tgcccagaag gtcgtcctga ccaagccgga
     3841 gcagaatgcc cgccgcgggc agcacggcaa tcggcaacat caggctcttg cccagtcgct
     3901 gcaattgcgc gaagccggga atgcgcagac ccgacttcgc cgatgccact tgaggtttcg
     3961 tcgtatcgcc catatcccgg accctcctgc ggtgcggatg tcacccctgc cgaagctgag
     4021 cggaagctta gaccagtttt cggcaccaac gtcgacggca actacttatc cttgggtgtg
     4081 agcaacacgc cagtccttgc cccgatcgag ggtcgcgccg ttccgcttga tgacgtcccc
     4141 gaccccgtct tctcccaggg catggtgggc tacggcgccg ctatcgaccc tccgcgcggg
     4201 gtgatcgatg cggtggctcc ggtctgcggc aagattttga agctgctccc gcacgcttac
     4261 atcgttatga ccccggaaaa tgtggggata cttgtccacc tgggtctcga caccgtccag
     4321 ctgaatggag agggcttcac cacccacgtc agccagggcg acaccgtcac cgcgggccag
     4381 gtcgtcgtca cctacgacgt cccgtcggtc gaggcgaaag ggctcaatcc cgttgtgccc
     4441 gtggtggtta tggacgaacg cgaacccggc aacgtcattc cgtccgaaat tgtcaccgcg
     4501 cgcgcgaaaa ttgcttgcgg cgcaactctt ttcacggcga acaagtagat ggaagtcatc
     4561 atcctgcagg acgccaagga gatcggcggc atcgccgccg actcgatcgg tgccctgctt
     4621 cgccgcaagc cgaccgccgt cctgggtctc gccaccggct cgtccccgct ggcgatttac
     4681 gacgaactgg tttctcgctg cgacgccggg ctgatttcat ttcggctggc ccgtggtttc
     4741 acactggacg aatacgtcgg tctgccagcc gatcatcctc agcggtatcg gaacgtcatc
     4801 gtcaacgtgc tcgtctcccg cgtcgatttc gctccgggag cggttcaggg ccccgacggc
     4861 ctggccgcgg atatcccgac ggcgtgtgca gagtacgaga acgcgatcca gcacgcgggc
     4921 ggagtcgatc tgcagattct cgggatcggc accgatgggc acatcggttt caacgagccc
     4981 gggtcttcac tcggctcccg aacgcgcatc aagacgctca cccgacagac ccgcctcgac
     5041 aacgcgcgct tcttcgacgg cgttgtcgac tcggtgccca cgcattgtct gacccagggt
     5101 ttggcaacga tcatggctgc caggcatctg atcctggtgg cgattggtcg ccgcaaatcg
     5161 gaagcggtgc accatttggt cgaagggccg gtcagcgccc tgtggccggc gaccatcctg
     5221 cagcatcacc cgcatgtgac ggcgttgttg gatgatgctg cggcagaacg gctccaactc
     5281 gctaattact accgcgaaac gtaccgctcg aagccggacc ggcagggcat ctgaggcgcc
     5341 accatgttga tgaccgctga caccctgctc accgggtcag aactgttgcg gccagcctgg
     5401 attgagattg agcgggggac ggtgcatgcg atcggatcag gggcaccgcc tcgtcttgca
     5461 gatcgcaacc tcggggcggt gactgtggtg ccgggtttcg tcgacaccca cgtgcacggc
     5521 gggggcggcg cgaacttctc caccgcatcc ccggccgaca ccgccaccgc agcaggactg
     5581 cacgttcggc acggcaccac catgctggtt gcttcgctgg tcaccgccag ccccgtcgat
     5641 ttgatacggc aggtcgggga gttggccgag gatgtccggc gcggtgtgat cgcagggatt
     5701 catctggaag gcccgtggct ggccgccgag cggtgtggtg cgcacgagcc gtcgctgatg
     5761 cgcgacccgg atcccgaaga actcgaccgt gttctcgcgg tgggcggtgc ggcgattcgg
     5821 atggtcactc tggcgccgga gcgggtcggc gcgctggcgg ccgtcaggcg aatcgtcgac
     5881 gcgggcgttg tcgccgcggt gggccacacc gacgcgacct atgaacagac gcgggcggcg
     5941 atcgcggcgg gcgcgacggt ggggacgcac ctgttcaacg cgatgcgccc gatcaatcac
     6001 cgcgagcccg gtccggtgat cgcgctgctc gaagactccc gtgtgacagt cgaactgatc
     6061 accgacggtg tgcacgtcga ccccgcgctg tatcgacacg tgtgccggag cgtggaacct
     6121 gatcgggtgt cgttggtgac cgatgcgatg gccgcggccg ggatggccga gggcaggtat
     6181 cggcttgggc cggtagcggt ggatgtcgtg gacggggtgg cgcgcgtggc gggcacgcaa
     6241 accatcgcag gcagcaccgc gaccatggac cgccaattca gatacgccgt caccgacagc
     6301 ggacttcctg gtgacgaggc gctgatggct gccgtgcgcc aagcgtcgat caatcccgcc
     6361 cgcgcattgg gtctgcccta cgcgggtctc gcccccgggg cgagcgccga cttggtggtg
     6421 ttggacgcag acctcgccgt gacgggcgtg ttgcgccagg ggctttgggt catagaaccc
     6481 gaaacggtgt cgtcgacccg atgacggggc gatcattgct tgaggggcaa gcggattgct
     6541 tcggaccaaa cccggccaag cggcgaagga gaatcgatga tcgaaaaact gtcagacatg
     6601 ccggagggag tgaccggcat cagcgtgtcg ggccgcctgc gcggtgacga cctgcgcgag
     6661 ttcaagccgg ccatggagga actcctgaag accggcgaga tcagaatcgt cgaggtcatt
     6721 gcgccagact acgaaggctt cgggcccggc gggatgatcg aggacctgaa gctgggcctc
     6781 ggtgccctga tccagcatca ttcggcgttc aagcgaattg cggtcgtgtc cgacaaagat
     6841 tgggtcgccc acgcgctgca tgccttcgcc tggatggtcc ccggcgagct cgcgctgttc
     6901 ggcctcgacg agctcgatcg ggccaagcag tgggccgccg gctgattctg agaggcccgg
     6961 tggaagcggc atgtcacgac tgtgggggta ccgacgcatc ctgcgccgga cgacgcagga
     7021 actcgacgag caccggcgct cgagtcgtcg tcgacggcgg cctcagcttt ccggtctggg
     7081 ctctcagtcg cggggatcgt cgaagctaga aacgcttccc cgactgaggt ggagacgcgc
     7141 accacgccga tgacaaccgt ccatcggata aatccctctg catcttaggt tctcacacaa
     7201 gaaggtcgac ttgccgaagc aactccatca cgtcgttatg tgtagtcatt cggcggacgc
     7261 actcgtgcgg ttcctcaccg acgtcgtcgg catggaagtc cagcaccagt tcagggtccc
     7321 cggcgaggtg ctggagaaaa ccttgggatg gccgccttct gatggagcgg acctgacgat
     7381 gcttggcagc ggcgtggcgg gcctgatcga ggtgctcgac gttcccgagc accttcgggc
     7441 cacggcaccc gagggactca ccgcactgtc gttcctgacc gacgacgtcc gcaatgtcca
     7501 ggacaaggcc cgtgactacg cgagcgacgt cacggtcctc gacgccgggg tgccaggcgt
     7561 cgacttgttc ttctgcaccg taggtggcgt acccatggag ttcatgggta gctatgaggc
     7621 tgagcgctaa tcggcgagcg cagattcgaa ttccacgacg gtcgccacgc gcgacttcga
     7681 gcgatcgaac aggcgtttct catcctcgag cacctcagga ggcggctcat tattggctgt
     7741 gtgcgtagct atcgcctcgg ccgtggcccg atccgccagc cacagttcgg tgatcacgtc
     7801 gaacggcaac tcctcgccgc cgaccggatc gacgtagcgc cgaacgtagc gcagcaaccc
     7861 ttgcgagtat ttctcgccaa tcttcgaatg gacgttttcg taatagtccc ggaaggcctc
     7921 tactgacatg ccgggccggc gcttgagaaa caacaacacc ttgtagaccg gctgcttccg
     7981 cggctcgttc atctcgaatt accttgcgct gccgcgcaaa acgcgcgaag ctcacgcgcg
     8041 acgtcaggtc cgctaggggt gtagatcagt tcttgcacgc cgcgatcctc caactgtgcc
     8101 aggctggctc ggatcttgtc gggctctccg atcatggtcg ggaacccgcc ggtgtagccg
     8161 accagcggat agtcccgctc cgtcaggtgc gtgacgtgcc cctcatacac gagcagatga
     8221 cgtccgcctt ccgcagatag tctctcgagc gcctcgcgcc actctcgccc gccgggcatc
     8281 gcgtcgactg ctgcggctcc ggcgacggca taggcgtcgt ggaacgccac cacttgccag
     8341 gggcctaccg cttcgcgtac tcgtggactg tcgcggtctt cacctggttc gaggaccgtg
     8401 cccgcgcgca tggtcgcaac cggaagcgat tggcgcacag gcaaaccgat gataccgtcg
     8461 gcaatgcgtg cggccaattc cgttgcgcgt ggaccgaatg cgctcagcca gacctccacg
     8521 tcgacgggcc gagacgccgt caggccgtcc gcgtgcagca tccgcacagg cgcgccctga
     8581 atcgtcactg tctcacccgc cagcagcccg cgcacagccg acacatattc acccaaaacc
     8641 cgcagagtca gcggccgctg gcccatggtc cggcgtgcgg tgccgccggt tccgaagcag
     8701 gcccgaaacc ggccgccgga gagtcgagcg atcgccgcaa tcgacgacgc catggccatt
     8761 tccgaccgct cggtcggaat caggaccgcg gtgccaagac cgatccggga cgtgcgttcc
     8821 gcggccagag cgaggtggac gaacgggtcc tcccacaggg gagcggagtc gaaaatccag
     8881 acccgtgaat agcccacatc ttcggccaga accgaaagat cgctgaacgc aggaccaggg
     8941 ggtagcccgc atgacagctc gatcgtcatc gaatcaacct cgcaacttgt tccggtcgaa
     9001 gaaggaactc gacgagaacc tctgccatct ccgaaatcgc ctcgcgggca atgacttcgt
     9061 agccgtgggc ccgcacgtcc acagcagctc ttgcagcaac tgaagctgca acccgcgatc
     9121 gtcgtcggtc acgaagcccc ggtggcgacc ggcggaactc gcccggcctc tgcggtatcc
     9181 agcaccagcg cggccactcg cgatcggtgt tgttcggcgg tgcccagcag cgcggcgtcg
     9241 gtggtcgcgc gtttgagata caagtgcgcc tggtgttccc aggtgaaccc gatgccgccg
     9301 tgcacctgga tggtgtccgc cgcgatccgg gagaacgcgg ccgagcaggt cgcttgcgcg
     9361 atgctcgccg ccagcgcggg gtcgtcggtg ccgtccgtca acgcccatac cgcgtgatag
     9421 gcggtggagc gggcgtgctc gagttcgacg agcagatcgg ccaaccggtg cttgaccgcc
     9481 tggaacgacc cgatcggccg gccgaactgc agccgcgact tcgcgtagtc aaccgacagg
     9541 tccagcagat gctgtgacgc gccgacctgc tcgacggcca gcagcgccga gcccacctgc
     9601 aacgcgtggc cgatcacgcg ctcggcctcg tcggccccgg cgaccagccg ggcgggggcg
     9661 tcggagaacc ggatcgtggc ctgcggtcgg gtgaggtcga gggtgaccaa cgggatccgc
     9721 tccacaccgg gcccggcggc ggcgacggca taaaggccca gcccgtctgg accgtcggcg
     9781 gccaccagca gatcgtcggc ggcgccgccg tcgaccaccc gctcgacggc gccggtcagt
     9841 gcgtcgccgt ccgcggtgac cgtgaccgct gccgggtcga acgccccggc ccagtcggcg
     9901 acggcgaacg cggcagttcg ccggccctcc gtcagctctg tcagcaactc ctcgcgaacc
     9961 ggccccgccg aggccgcgac gagtgcgggg acggccagga acacggtgcc gaaaaccggg
    10021 ccgcacgcca gcgacgcggc caactgctcc accgctagtg cctgatccac cagggtgccg
    10081 cccgccccgc cgccgctctc gggcaccgac agcccgagca ctcccagctc cgaaccgagc
    10141 cgagcccaca ccttcgggtc gaacggggga tcggactcca tcagacggcg caccgtctgc
    10201 tcgtcgaagt tttcggcgca aaaccgccgc accgcgtcgc gcagctgcgc ctgctcctcg
    10261 gtgaacgtga actcagccac gggggatctc cttccacggc atgccggcgt cggcgcgcag
    10321 gtcgccgggc aggcccagca cccgctcgcc cagaatgttg cgcatcacct ccgaggtgcc
    10381 gccctcgatg gtgttggcgc ggctgcgcag aaaccgctgc tgcaccgggc ctcgccagtc
    10441 ctcgacgtcc tggtcgctgt cccgcatggc gtagctgtgg tagagaatcc cttcccgccc
    10501 aaggaaatcc atgcagaact gatagatgta ctgattcagc tcggcaccga ccagcttgcc
    10561 gatggaaccc tccggccctg ggccgcccac ggtcgccgcg gcccgtgacc gctccgacgt
    10621 gaggcgttgc gcctcggcgc gcagccacaa ctgggtgagc cggtcacgca gcaccggcgt
    10681 ctggaggtcc gggcgggagg cccacagcga gacggcatcg gcgatggtcc cggccccgcg
    10741 ccggctgccg ctggcgccga gtgcgctgcg ctcgttcatc agcgtggtca tcgccacccg
    10801 ccagccgtcg ccgacctcgc cgagccggta cgcgtcgggg atgcgcgcat cggtcatgta
    10861 cacctcgttg aactcggcgt gaccggtcag ctggcgcagc ggccgggtct cgacacccgg
    10921 cgcgtgcatg tccaggacga agtaggtcag ccctttgtgt ttgggcatgt cgggattggt
    10981 gcgcgcgagc agcagtcccc agcgcgcgcg gtgcgcgagg ctggtccaga ccttctgccc
    11041 gttgaccacc cattcgtcgc cgtcgggcac ggccgaggtc gcgagcccgg ccaggtcgga
    11101 gccggcaccc ggctcggaga acagctggca ccagatgtcc tcggtggtcg ccagcggccg
    11161 gagcaagcgt tgcttcacct cgtcgctccg cgcgtgctct cgcactgtgg gtgccgccat
    11221 cccgtagccc atcgggttca acgccagcgg caccgggccg cccgcaccct gcagaatccg
    11281 gtcggcgacg gcctgcagac cgcgcgacac cccgaggccg ccgagtccct cgggaaagtg
    11341 cacccaggac aggcccgcgt cgaagcaggc gccgagaaat tcagggatgg gaaccgattt
    11401 cgggtcatgg tctcgcacga cgtgtcgcgc gaggtcggcc acccggtcgg cgtcagcaac
    11461 agtgctcatc cagtcacgat agaaaaccgc cagtgtcgag acgaagagtg tcaccggatt
    11521 gcggcccata cactcgtcac gtggcccgtg aggatcgcga ccggctctcc gttgacgatg
    11581 cgcgcatcct ggggctcgag tccgcgacga tcaccggaca taccctgaag ctcatcgtgc
    11641 tcgagccggg gaccggtccg ctcgacctcg tcgcccttca ggctgaagtg gcgaagcgac
    11701 tgcccaccca accgcgcgcg acgcagcgcg tcgacgtatc cggaaatgaa ccgcgatggg
    11761 tcgacgcaac cgatttcgac atccgcgaac acgtccggcg gcaggcgaag tccgaatgcg
    11821 cctcgcggga tgacctctgg cgggccgtga gcgcgttgat gtccgagcac ctcgaccgcg
    11881 agcggccact gtggactttc gacctgatcg gcccacttcc cgacggccgc gaagcgatcg
    11941 ccgtccgcat ccaccacgcc atggccgacg ggatcgccgc ggtccggttc ttggaggccg
    12001 ttctgtggga cccacacctc gatgagccgg ccggggagcg ggcgcaaccg ggcctgcgct
    12061 cggctccggc gccctcgtgg ctcgccgagg cgtggcggat gcccggtgcg gtccgtcgtg
    12121 agctcggcca ccccggctcg cgctcgccgt tcgaccgccc gatcggcggc tcgcgggcct
    12181 tggccttcac ggtcgcttcg ctggcagagc tgaaggcgat cggtgcgtcg cgccccgcgc
    12241 acaccacggt taacgacgtc ctcctcacga tcgtcgccgg cgggctgcgc gcgtggctcg
    12301 cggccggcga agcggccaca ccgcaccttc acgcccaggt tccggtcagc ctccaccacc
    12361 gcgatgaggg agcggacgat ctcggaaacc gcgactcctt catcaacgtc gatctgccgc
    12421 tcgccgaggc ggatccactc gtgcgactcg acctgatcag cgccgagacc agcaagcgca
    12481 agcgcctggg cgacgccgag gagctgtacg acctcttcca cgcgctcggg cggatgaagt
    12541 acgtcggtag tgccgtgcag cgactcgcca agagcgcgcg cgaattcagc gtcgcgatct
    12601 cgaacgtccc gggtccatcc gcaccggtca gcgtcgtgcg gcgccgagtg caacacctct
    12661 tctcctcgtc agagccggcc acccaccacg cgctgcggat ctcggcgatc tcatgcgccg
    12721 gggacatcgg gatcggcctg tgcaccgacc cgcaggcgct gccggatatc gcgcggctcg
    12781 cggaagcgat cgagcgctcc tatgccgagt tgcgcagtgc ggcaatcgga taaccgcaaa
    12841 cgggtggtgg tcgccgggct cggcgatacc ggggtcctga ccgcgatcca cctttcccgt
    12901 cacgccgacg tcgtgggcat ctcggtcaag ccggcgttgg tgagcggcca agaactgggt
    12961 gtgcgcctgt cccgcccgga cgactgggcc cgtgactact ggatcgcatt cgacaggttc
    13021 cgccggctgg atcgcgtgcg aacggtccat gccgcgctgg ccggggtcga ctttgccgct
    13081 cgaaaagtgt tcggccagag ggccgatggc ttcgccctgg ctgaagggta cgacgcgctg
    13141 gtcatcgcga caggcgtcgc gaacggcttc tggcgccgac cgaccttaca gtcgcaggga
    13201 gagatcggcg cggaacttcg tgctgcacat gatcgattgg ccgccgcgaa atcggtgatc
    13261 gtggtgggcg gcggtgccgc agccgtcagc agcgccgcca atctcgcgat gacatggccg
    13321 gccacgcgtg tcgggctgta cttccccggg gaacgcgctc tggtccgcca ccacccgcgg
    13381 acatgggaac ggatccggcg acgactgacc gagatcggtg tggtgctgca cccaggacac
    13441 cgtgcggtgg tgctgcaggg tttcgcgtgt gaccagatca ccagcgagcc cgtgcagtgg
    13501 agcaccggac aaccgtccac atccgccgat gccgtgcttt gggcgacggg acgagtgcga
    13561 cccaacacgg actggttgcc ggcggaactg ctcgacgatg acaatttcgt ccgggtgaca
    13621 ccggaattgc gggttccgca tcacgccggt gtcttcgcgg tcggcgacgt ggcggcgacc
    13681 gacccacttc ggtcctccgc ccgcaacaga gcggatcgct ctgtcgccca caatgttttg
    13741 gctgagttcg gcggtcggcc gatgcgcact ttccgtgcgc cgcaacggcg atggggttcc
    13801 gtgcttggca tccagcccga cggactcgag gtcttcagcc ccaccggtca tgcgttccgc
    13861 ttcccggcgt ggtcgaccga ccgcgtgctg atgccgtgga tcgtccgccg cggcatctat
    13921 cgcggagtgc gggagaaccg catcaccgca tcgtgaagcc ggcgtccaac ggccacgccg
    13981 tgccggtgat gaaccgtgcg tcgtcggaaa ccagccacgc caccgcgccg gccacctctt
    14041 ccggctgcag cagcgggatc ggcaacgcgt tctgcatgtc ggcgagccac ggatcgtttg
    14101 ccgccgccat ggtcatcgtc gtctcgttga ggatcatgtc ggtggccacc ccgctcggat
    14161 gaatggtgtt gacccgaata gactgcgggg cgaggtactt cgcccagccc tgcatcagcg
    14221 ccaccaaacc ccgcttcgag gcggcgtagg cctgcagccc cggccggtcg gtgctggaag
    14281 cgtccagccc cgcggtcgaa ctcgccagca cgatggatcc gccccggtta ccgccgacca
    14341 gagcgggaat cgcggcttcg accgtgttcc aagcaccgat caagttgacg tcgacgatgt
    14401 ctcggaacgt ggcatccggt tcgtcgctgt cggtcagccg gatgatcccg gcgttggcga
    14461 tgacgatgtc gaggcggccg aaatgttcga cggcttcacg caccaccgca tcgaccgcgt
    14521 cgcggtcgcg cacgtcggca tgccgcgaca gaatcgtgcg cccctgagct tggacgagtt
    14581 gctctgtgcg ggtgaggtct tcgggtgtcg agccggggta gtcggtggtg gcgaagtcct
    14641 ggcagatatc cacggcgatg atgtcggcgc cttcggttgc gaacttgacc gcataggcgc
    14701 gacccagtcc gcgcgcagcg ccggtgatga aggcgacttt gccgtccagg gtgcccatcg
    14761 tcacgcaccg cggaagttga cgtcacgcgt gacggcttcc cactcgtcga tcgaggcggt
    14821 catcgcggcg atcttgtccc gcatcgcttt cagcacgtcg cggccgagca gcagccgcag
    14881 tggcggctcg tccagcgtgg tcaccatcag caccgcctcg gccactttgc gtgggtcgcc
    14941 gggaaggtga ttggcgaact ccttgatcat cgtcttgcgt tcgcccacgt tctcctcgta
    15001 atcggtgatc ggcgtcgacg tctccttcat cgaacgcgcc gcccagtccg tgcggaaggc
    15061 gccgggctcg atggcggtca ccttgatgcc gagcggcttg acctccgccg acagcgcctc
    15121 ggtcaacgcc tcgagcgcga acttcgtcga cgagtagtac gcgttcggcg ggttggccac
    15181 cagacccgtc atcgaggaga tgttcacgat gtgacccgac cgaccggcgc gcatggccgg
    15241 cagcaccgct ttgatcatgt cgaccacacc gaagtagttg atgtcgaaca gctttcggac
    15301 ttcagcgtcc tcaccctcct ccaccgcgga gaggtatccg tggccggcgt tgttcaccaa
    15361 gacatcgatg ccgccgaacg cctcgtcggc cgcctcaacg gcggcggcga tctggctctt
    15421 gtccgtcacg tcgagggcga cgctcaccgc gtggccgccg aactcgtcga cgatgtcctg
    15481 aaccgtctcg ggccgccgcg cggtcaccac gacgtcgtgg ccggcttgca gtgcggcaac
    15541 ggcgatttca cgtccgatgc cggtggagca tccggtgatc aaccagcggg ccatgtcaga
    15601 gggtcccttc cggcagccgc cgcagatatc cagcccgccg gtgtgcaagc tcggtcgctc
    15661 gctcttccga actcacccag cgcaagtgcg gcacttcgct gtccagggcg tcgagcttga
    15721 ccacccgccc gcgcgcaccg agatcctctg tcggactgcc ttcaccgaac tccgccatcc
    15781 gcagcgtcag aatgccctcg ctcagaccgt cggagtcgat ccagttctgc acgccggggt
    15841 cgacggcgga gatgacgtag gtgtacgtgc cgtcctcgtt ggccaccgac tgagccttgt
    15901 tgagactgcc cgtgcggtcg acgatgtcg
//
LOCUS       DASWBF010000085         7934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1122797,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000085 DASWBF010000000
VERSION     DASWBF010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122797"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VF874_02655"
     CDS             complement(<1..562)
                     /locus_tag="VF874_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9175021.1"
                     /translation="MAQRPPTGSTVVKLAWCCLLAGVLAAALMFPFAGGVGLLSNRAS
                     DVVANGSAQLVEGDVPQVSTMVDAAGNPIAWLYTQRRFEVPTDQIADTMKLAIVSIED
                     KRFAEHNGVDWQGTLTGLAGYASGDTDTRGGSTIEQQYVKNYQLLVTAQSDAERRAAV
                     ETSPARKLREIRMALTLDTTFSKPEIL"
     gene            953..1261
                     /locus_tag="VF874_02660"
     CDS             953..1261
                     /locus_tag="VF874_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9175022.1"
                     /translation="MESSVQAAATEARIAWVSKARCRSTDPDELFVRGAAQRKAAVIC
                     RHCPVLAECGADALDNRVEFGVWGGMTERQRRALLKQHPEVVSWAEFFAAQRKHRNAV
                     "
     gene            complement(1306..2433)
                     /locus_tag="VF874_02665"
     CDS             complement(1306..2433)
                     /locus_tag="VF874_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX9175023.1"
                     /translation="MSTIPPTLDMASILADTSNRVVVCCGAGGVGKTTAAAAMALRAA
                     EYGRTVVVLTIDPARRLAQALGIANLGNSPQRVPLAPEVKGELHAMMLDMRRTFDEMV
                     AEYSGPERAQSILDNQFYQTVATSLAGTQEYMAMEKLGQLVSQDKWDLVVVDTPPSRN
                     ALDFLDAPKRLGSFMDSRLWRLLLAPGRGIGRLVTGAVGLAMRAMSTILGSQMLSDAA
                     AFVQSLDSTFGGFRQKADRTYALLKRRGTQFVVVSAAEPDALREASFFVDRLSQEAMP
                     MAGLILNRTHPTLSGLHLERAVDAAEELEADDPDSLAAAVLRIHADRAQTAKREVRLL
                     SRFTGANPHVPIVGVPSLPFDVSDLDALRAIADQLTAAKVA"
     gene            complement(2430..3449)
                     /locus_tag="VF874_02670"
     CDS             complement(2430..3449)
                     /locus_tag="VF874_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX9175024.1"
                     /translation="MATTREAGPEVGWPARLRDARLHFVTGKGGTGKSTIAASLALAM
                     AAGGRRVLLVEVEGRQGIAQLFDVPPLPYQEVKIATADRGGLVNALAIDIEAAFLEYL
                     DMFYNLGLAGRAMRRIGAVEFATTIAPGLRDVLITGKIKEVVVRTDKSRLPAYDAVVV
                     DSPPTGRIARFLDVTKAVSDLAKGGPVHSQADGVVQLLHSDQTAIHLVTLLEALPMQE
                     TVEAIDELQKMQLPIGSVIVNRTLPVYLQPDDLAKAAEGDIDADTVRADLTKVGVKLD
                     DDDFAGLLTETIQHATRLKARAEAAEQLDEINVSRLELPNLADGVDLGSLYQLAEDLG
                     RQGVR"
     gene            3498..3659
                     /locus_tag="VF874_02675"
     CDS             3498..3659
                     /locus_tag="VF874_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEX9175025.1"
                     /translation="MSEPTRWEYATVPLLTHVTKQILDQWGEDGWELVAVLPGPTGEQ
                     HVAYMKRPK"
     gene            3656..4111
                     /locus_tag="VF874_02680"
     CDS             3656..4111
                     /locus_tag="VF874_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9175026.1"
                     /translation="MTHSSRLAELGIELPTVAKPLAAYVPAVRTGNLVYTAGQLPLVS
                     GNPAVTGKVGSEVTPEVGKTLARVCALNALAAVDALVGVDAITRVVKVVGFVASAPGF
                     TGQPGVINGASELFAEVFGDAGAHARSAVGVAELPLDAPVEVEVIVEVA"
     gene            4134..4907
                     /locus_tag="VF874_02685"
     CDS             4134..4907
                     /locus_tag="VF874_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9175027.1"
                     /translation="MIEHPAYGVLRPVTETASVLLCDNPGLMTLEGTNTWVLRGPGSD
                     EVVVVDPGPDDDEHIARVVEHGTVALVLISHRHEDHTEGIDKIVDLTGAVVRSVGSGF
                     LRGLGGPLTDGEVIDAAGLRITVMATPGHTADSLSFVLDDAVLTADTVLGRGTTVIDS
                     ADGSLVDYLESLRRLRALGNRVVLPGHGPDLVDLAAVADMYLAHREERLNQVRDALKA
                     LGDDATTRQIVEHVYTDVDEALWDAAESSVQAQLDYLRD"
     gene            complement(4984..5658)
                     /gene="crp"
                     /locus_tag="VF874_02690"
     CDS             complement(4984..5658)
                     /gene="crp"
                     /locus_tag="VF874_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HEX9175028.1"
                     /translation="MDEILARAGIFQGVEPTAVAALTKQLQPVDFPRGHTVFAEGEPG
                     DRLYIIISGKVKIGRRSPDGRENLLTIMGPSDMFGELSIFDPGPRTSSATTITEVRAV
                     SMDRDALRAWIGDRPEIAEQLLRVLARRLRRTNNNLADLIFTDVPGRVAKQLLQLAQR
                     FGTQEGGALRVTHDLTQEEIAQLVGASRETVNKALADFAHRGWIRLEGKSVLISDSER
                     LARRAR"
     gene            complement(5759..6043)
                     /locus_tag="VF874_02695"
     CDS             complement(5759..6043)
                     /locus_tag="VF874_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175029.1"
                     /translation="MLATFGLHRVESHLNRECVGAADVAEFLAQARPGDMRTLARDGM
                     PEALDSIRQRRTLRGVDPSWQVAHATRGCPQSVPAMANPQFRPTRHADSV"
     gene            6235..6915
                     /gene="nth"
                     /locus_tag="VF874_02700"
     CDS             6235..6915
                     /gene="nth"
                     /locus_tag="VF874_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9175030.1"
                     /translation="MNRTLAQAFPHVYCELDFTAPLELTVATILSAQSTDKGVNLTTP
                     ALFARYRTARDYAQADRTELEELIRPTGFYRNKASALIKLGQALEERFDGKVPATMDE
                     LVTLPGVGRKTANVILGNAFGVPGITVDTHFGRLVRRWRWTDETDPVKVEKAVGELIE
                     RCEWTLLSHRVIFHGRRVCHARRPACGVCVLAKDCPSFGLGPTEPEIAAALVRGPETD
                     HLLALAGL"
     gene            6929..7564
                     /locus_tag="VF874_02705"
     CDS             6929..7564
                     /locus_tag="VF874_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9175031.1"
                     /translation="MARSTRWTIAVLVVVAAIIVALALQLRDTAPSSAPSGPSAGAPQ
                     GSGDLTALRRRADLPPCPPGGSGPGPQSLRGVTVQCLADGATVDTAGAVAGRPVLLNL
                     WAYWCQPCAQELPALAEYQRRVGDRITVITVHQDDDAAAALSRLADWGVRLPTWQDGQ
                     RHVASALGVPNVMPTSVILRTDGSVARILPRAFASADEIAAAVDQTMGAPA"
     gene            7591..>7934
                     /locus_tag="VF874_02710"
     CDS             7591..>7934
                     /locus_tag="VF874_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9175032.1"
                     /translation="MTSAGPTGVLTPHAAPAWLKPLVENVGCVPAAYRRRLPAEVLAM
                     VTAANTAAKLTGSPRDAAVLALFSGPEDAPVDGGVPDEADLLLTVRASSLRHHAGQAA
                     FPGGAVDSGDVGP"
BASE COUNT         1282 a         2663 c         2692 g         1297 t
ORIGIN      
        1 tcaggatctc cggcttggag aacgtcgtgt cgagcgtcag cgccatccga atctcacgca
       61 gcttgcgcgc cgggctggtt tccaccgcgg cccgccgttc ggcgtcgctc tgcgcggtca
      121 ccagcaactg gtagttcttc acgtactgct gctcgatcgt cgacccgcca cgggtatcgg
      181 tgtcgccgga ggcgtagccg gccaggccgg tcagcgtgcc ctgccagtcc acgccgttgt
      241 gctcagcgaa ccgcttgtcc tcgatcgaga cgattgccag cttcatcgtg tcggcgatct
      301 ggtcggtggg tacttcgaac cggcgctggg tgtacagcca ggcgatcggg ttgcccgccg
      361 cgtcgaccat cgtggacacc tgagggacgt cgccctccac caattgcgcg gacccgttgg
      421 cgacgacatc ggatgcgcgg ttggacagca acccgactcc gccggcgaac gggaacatca
      481 gcgccgcggc caacacgccg gcgagaaggc agcaccacgc caacttgacg accgtcgacc
      541 cggtcggggg gcgctgcgcc atgcgttata ggttactgat gttgtcgcgg cgtcgtccgc
      601 gacggccagt gagggccaaa ctcttggctt tcgcgtgttt gcgcagcata attggcatgc
      661 gcgtcaggtt tttgctagcg cgaggggctt gcgcagaggt cgagaggata cgcggaaggg
      721 gcttgacggc acggtcgtcc caaaaaaagc ggtatcaaac tgttgcgcaa acgatacctg
      781 accacctaat ttgtacttct agtgcgatat agataacaag aacctgcaca gtgtggcgta
      841 gatcgcatta cgagatcgac gccggggcgc cgccattacg acggctggaa cgaaggggac
      901 gctggtgtca ggtactcggc ccgcagcgcg gaggacaacg gggggaagtt cagtggagag
      961 ttcagtgcag gcggcggcga cagaggcccg catcgcatgg gtatcgaagg cgcggtgtcg
     1021 ctccaccgac cccgacgagc tattcgttcg cggggccgcg cagaggaagg cggccgtgat
     1081 ttgccggcac tgtccggtcc tcgccgagtg cggcgccgac gcgttggaca accgggtcga
     1141 gttcggggtc tggggcggga tgaccgaacg ccagcggcgc gcgctgctca agcagcaccc
     1201 tgaggttgtt tcctgggccg agttcttcgc cgctcagcgc aaacaccgca acgcggtcta
     1261 agacctgcca gcggcctgct cgtcagggcc gcgctacaga cccccctacg ccaccttggc
     1321 ggcagtgagc tgatccgcga tcgcgcgcag cgcgtcgagg tctgacacgt cgaacggcag
     1381 cgacggcacg ccgacgatcg gtacgtgcgg gttggcgccg gtgaaccttg acagcagccg
     1441 cacctcacgc ttggcggtct gggcccggtc ggcgtggatg cgcagcaccg ctgccgccag
     1501 cgagtcggga tcgtcggcct ccagctcttc tgcggcgtcc accgcgcgtt cgaggtgcag
     1561 cccggacagc gtcggatgcg tgcggttgag gatcaggccg gccatcggca tggcctcctg
     1621 ggagagccgg tcgacgaaga acgacgcttc ccgcagcgcg tcgggctcgg ccgccgacac
     1681 cacgacgaac tgggtgccac ggcgcttcag cagcgcgtag gtgcgatcgg ccttctgtcg
     1741 gaacccgccg aacgtggagt ccaacgactg cacgaaagcg gctgcgtccg aaagcatctg
     1801 cgaacccagg attgtcgaca tcgcccgcat ggccagcccg accgcacccg tcaccagccg
     1861 tccgatgccc ctgccaggcg ccagcaggag gcgccacagc cggctatcca tgaagctgcc
     1921 cagccgtttt ggtgcgtcga ggaagtccag ggcattgcgc gacgggggag tgtcaaccac
     1981 gacgagatcc catttatctt gggaaacaag ttgtcccagc ttctccatcg ccatgtattc
     2041 ctgtgttccg gctagcgacg tggcaacggt ctgatagaac tggttatcca aaatcgattg
     2101 cgcgcgttca ggtccggagt actcggccac catctcgtcg aacgttcggc gcatgtcgag
     2161 catcatcgcg tgcagttcgc ctttgacctc cggcgccagc ggcacccgct gtggcgagtt
     2221 gccgaggttc gcgatgccga gagcctgcgc cagccgccgg gccgggtcga tcgtcaaaac
     2281 cacgaccgtg cggccgtatt cggccgcgcg cagcgccatc gccgcggcgg cggtggtctt
     2341 gccaacgcca cccgcaccac agcagaccac gacgcggttg gaggtgtccg cgaggatcga
     2401 cgccatgtcc agcgtcggcg gtatcgtgct catcgaaccc cctgtcgccc aagatcttcc
     2461 gccaactgat aaaggctgcc gaggtcgaca ccgtcggcga gattcggaag ttcgaggcgc
     2521 gatacgttga tctcgtcgag ctgttcggcg gcctcggcgc gcgccttcag ccgggtggcg
     2581 tgctggatag tctcggtgag caggccggcg aagtcgtcgt cgtcgagctt cactccgacc
     2641 ttcgtcaggt ccgcccgcac cgtgtcggcg tcgatgtcgc cctccgcggc cttggcgagg
     2701 tcgtctggct gcaggtagac cggtagcgtc cggttgacga tcacgctgcc gatcggtagc
     2761 tgcatcttct gcagctcgtc gatcgcctcg acggtctcct gcatcggcaa cgcctccagc
     2821 aatgtgacca ggtggatcgc cgtctggtcg gagtgcagca gttgcaccac gccgtcggcc
     2881 tgcgaatgca ccgggccgcc cttggccagg tcggacaccg ccttggtgac gtcgaggaat
     2941 cgggcgatcc ggccggtcgg cggagagtcc accacgaccg cgtcgtaggc cggcagccgc
     3001 gacttgtccg tgcgcaccac gacctccttg atcttgccgg taatcagcac gtcccgaagt
     3061 cccggcgcga tcgtcgtcgc gaactcgacg gcgccgatgc ggcgcatcgc gcgcccggcc
     3121 aaccccaggt tgtagaacat gtcgaggtat tccaagaacg ccgcctcgat gtcgatggcc
     3181 agcgcgttga ccagcccgcc gcgatcggcg gtggcgatct tcacttcctg gtagggcagc
     3241 ggcggcacgt cgaacagctg cgcgatgcct tggcggcctt cgacttcgac cagcagcact
     3301 ctgcgcccgc cggcggccat ggccagcgcg agtgaagccg cgatcgtcga ctttccggtg
     3361 ccgcctttgc cggtgacgaa atgtaggcgt gcgtcgcgca gccgcgcggg ccagccgact
     3421 tcgggtcccg cctcccgtgt ggttgccacc ttcgcagcct aaccaaccgc atggttgcga
     3481 cagctagggt tagccccatg agcgaaccga cccgctggga gtatgcgacc gtcccgctgc
     3541 tgacccacgt gaccaaacag attttggacc agtggggcga agacgggtgg gagctggttg
     3601 ccgtgcttcc cgggcccacc ggcgagcagc acgtggcgta catgaaacgg ccgaagtgac
     3661 ccactcgtcg cggctggccg agctgggcat cgaattgccg accgtcgcca agccgctggc
     3721 cgcatacgtt cccgcggtac gcaccgggaa cctggtgtac acggcgggcc agctgccgtt
     3781 ggtgtccggc aacccggccg tcaccggcaa ggtcggcagc gaggtgacgc cagaggtcgg
     3841 caagacgctg gcacgggtgt gcgcgctaaa cgccttggcg gccgtcgacg cgctggtcgg
     3901 tgtcgacgcg atcacccgag tggtcaaggt cgtcggcttc gtcgcgtcgg cgcccgggtt
     3961 caccggccag cccggggtga tcaacggcgc gtcggaactc tttgccgagg tgttcggcga
     4021 cgccggggcg cacgcccggt ccgcagtcgg ggtggccgag ttgcccctcg atgcgcccgt
     4081 cgaggtggaa gtcatcgtcg aggtcgcgtg agcgacaatt cagcagaggt gacgtgatcg
     4141 agcatcccgc ctatggcgtg ctgcgcccgg tgaccgagac cgcgtcggtg ctgctgtgcg
     4201 acaacccggg gctgatgacg ctggagggca ccaacacgtg ggtgttgcga ggacccggca
     4261 gtgacgaggt cgtggtggtc gatcccggac cggacgacga tgaacacatc gcccgtgtcg
     4321 tcgagcacgg cacggtcgcg ctggtgctga tcagccaccg ccatgaggac cacaccgaag
     4381 gcatcgacaa gatcgtcgac ctcaccggcg ccgtcgtgcg ctctgtgggc agcgggttcc
     4441 ttcgtgggct gggcggtccg ctgaccgacg gcgaggtgat cgacgcggcc ggactgcgca
     4501 tcacggtgat ggcgacgccc gggcataccg cggactcgct gtcgttcgtg ctggacgacg
     4561 cggtgctgac cgccgacacg gtgttgggcc gcggcaccac ggtcatcgat tcggctgatg
     4621 gcagcctcgt cgactacctg gagtcattgc gccggctgcg cgcgctcggc aaccgtgtcg
     4681 tgcttcccgg acacggcccc gacctggtgg acttggccgc ggtcgccgac atgtacctgg
     4741 cgcatcgcga agagcggctc aaccaggtgc gggacgcgct gaaggcgctc ggcgatgacg
     4801 cgaccacccg ccagatcgtt gagcacgtct ataccgacgt cgacgaggcg ttgtgggacg
     4861 ccgcggaatc cagcgtccaa gcccagctgg actacctgcg cgactgagct ttcgcgcgaa
     4921 acggtattcc acgtggtctc cactcgcact ttcccgcgtg gaataccgtt tcggcgcaag
     4981 cgactagcgg gcgcggcggg ccaaccgttc cgagtcggag atcagcacgc tcttgccctc
     5041 cagccggatc cagccgcggt gcgcgaagtc ggcgagcgcc ttgttaaccg tctcccgcga
     5101 cgcgccgacc agctgcgcga tctcctcctg cgtcaggtcg tgggtgacgc gcaacgcgcc
     5161 gccttcctgc gtgccgaacc gctgcgcaag ctgcagcagc tgcttggcca ctcggcccgg
     5221 cacgtcggtg aagatcaggt ccgcgaggtt gttgttggtg cgtcgcagcc ggcgggccaa
     5281 aacccgcagc aactgctcgg cgatctccgg ccggtcgccg atccaggcgc gcagcgcgtc
     5341 gcggtccatc gacaccgcgc ggacctcggt gatcgttgtc gcgctcgagg tccgcggtcc
     5401 cgggtcgaag atcgacagct cgccgaacat gtccgacggg cccatgatcg tcagcaggtt
     5461 ctcgcggccg tccggagagc gacggccgat cttgaccttg ccggagataa tgatgtacag
     5521 ccggtcgccc ggttcgccct cggcgaagac ggtgtgcccg cgcgggaagt cgaccggctg
     5581 gagttgtttg gtcagcgcag ctacagcggt gggctcgaca ccttggaaga ttccggccct
     5641 ggccaggatc tcgtccacgt tgcccctctt aagctctcgg atgatcagaa gcgcgaaccg
     5701 accacctagt tcggcggcga accgatccag cggtcggtgt gtctatgacc ctttgacgct
     5761 acacagaatc cgcatgtcga gtcggtcgaa attgcggatt ggccatcgcg ggcaccgact
     5821 gggggcagcc gcgcgtggcg tgcgctacct gccacgacgg gtcgacgccc ctgagggtgc
     5881 gccgctgcct gatggagtcc aacgcctccg gcatgccgtc ccgggcaagc gtgcgcatgt
     5941 cgccgggccg cgcctgcgcc aggaactccg ccacgtcggc ggctcccacg cattcgcggt
     6001 tcaggtggct ttcgacgcga tgcagcccga aggtcgccaa catcagcaac ccagggatga
     6061 gcgcgactaa aagccatggc acaggcagaa gtaaacacgc caaaggtttc agcgtgatta
     6121 cagaatccca ccggtcagta cgcttgcctg gtgactgcga gcaggcggac cgcgcccacc
     6181 gagcggcggt gggccgccga gacgcgcctg ggcctggtgc gacgggcccg gcgaatgaat
     6241 cgcacactgg cacaagcctt tccgcacgtc tactgcgagc tggacttcac tgctccgctt
     6301 gagctcacgg ttgcgacgat cttgtcggcc caaagcacag acaagggtgt aaatctgacc
     6361 actccggcgt tgttcgcccg ctaccgcacc gcccgggact acgcgcaggc agaccgcacc
     6421 gaactcgaag agctcatccg gcccaccggc ttttaccgca acaaagccag cgcgctgatc
     6481 aagctgggcc aggcgctcga ggagcggttc gacgggaagg tgccggcgac catggacgag
     6541 ctggtgacgc ttcccggcgt gggccgcaag accgccaacg tcatcctcgg caacgcgttc
     6601 ggggttcccg gcatcaccgt cgacacccat ttcgggcggc tcgtgcggcg ctggcggtgg
     6661 accgacgaaa ccgacccggt gaaggtcgaa aaggcggtgg gcgaactgat cgagcgctgc
     6721 gaatggacgc tgctcagcca ccgcgtgatc ttccacggcc ggcgggtctg ccacgccagg
     6781 cgaccggcct gcggggtctg cgtgctggcc aaggactgcc cgtcgttcgg actgggcccc
     6841 accgaaccgg aaatcgccgc cgctctggtg aggggcccgg aaaccgacca cctgctggca
     6901 ctggccgggc tgtaacacgc caagcgccat ggccaggtcg acccgctgga cgatcgctgt
     6961 gttggtcgtg gtcgcggcga tcatcgtcgc gctggcgttg cagctgcggg acaccgcacc
     7021 cagcagcgcg cccagcggcc cgagcgccgg cgcacctcaa ggaagcggag acctgactgc
     7081 cctgcgtcgt cgagctgacc tgccgccatg cccgccgggc ggcagcggtc cggggccgca
     7141 gtcgctgcgc ggtgtgacgg tgcagtgcct cgccgacggc gcgaccgtcg acaccgcagg
     7201 agcggtcgcc ggacgaccag tgcttctgaa cctgtgggcc tactggtgtc aaccgtgcgc
     7261 tcaggagctg ccagctctcg cggagtacca gcgccgggtc ggcgaccgga tcacggtgat
     7321 caccgtgcat caggacgacg acgccgcggc ggcgctgtcc cggctggcgg actggggcgt
     7381 caggttgccg acgtggcagg acggccaacg acacgtcgcc tcggcgctgg gggtcccgaa
     7441 tgtcatgccc acgtcggtta tcttgcgtac ggacggtagc gttgcgcgca tcctgccgcg
     7501 ggccttcgcc agcgctgacg aaatcgccgc cgcggtcgac cagacgatgg gagcgccggc
     7561 gtgagctcgg agccgcaggg ccggcgacat atgacttcag cgggtccgac gggcgtgctc
     7621 acaccgcatg ctgcgccggc ctggcttaaa ccactcgtcg aaaacgtcgg atgcgtaccc
     7681 gcggcctacc gccgccggct gccggccgaa gtgctcgcca tggtgaccgc cgccaatacg
     7741 gccgcgaagc tcaccggatc gccccgcgac gccgcggtgc tggcgctgtt ctcggggccc
     7801 gaggacgcac cggtcgacgg cggcgtgcct gacgaggccg acctgctgtt gaccgtccgt
     7861 gcctcgtccc tgcgccacca cgccggtcaa gccgcctttc ccggcggcgc tgtcgattcc
     7921 ggcgacgtcg gccc
//
LOCUS       DASWBF010000086        12419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1192659,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000086 DASWBF010000000
VERSION     DASWBF010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12419
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192659"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VF874_02715"
     CDS             <1..1401
                     /locus_tag="VF874_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX9175033.1"
                     /translation="GGTGATPLTSMKHAGAPWELGLAETQQTLLLNGLRDRIVVQVDG
                     QLKTGRDVMIATLLGAEEYGFATAPLVVSGCIMMRVCHLDTCPVGVATQNPELRKRYN
                     GKPEFVENFFMFIAEEAREYLAQLGFRTINEAVGQVGALDTTVAAEHWKARKLDLSPV
                     LYEPESAFMNQDYYCSSRQDHGLDKALDQQLIVMSREALDAEKPVKFSTTISNVNRTV
                     GTMLGHELTKAYGGQGLPNDTIDITFDGSAGNSFGAFVPKGITLRVYGDANDYVGKGL
                     SGGRVVVRPSRNAPDAYVAEDNIIAGNVILFGATSGEAFLRGQVGERFAVRNSGAHAV
                     VEGVGDHGCEYMTGGKVVILGPTGRNFAAGMSGGVAYVYDPVRMLPDHLNSEMVELEE
                     LDDDDFEWLYGMLTRHVDATESAPGTRILSHWPTERARFVKVMPRDYKRVLEAIAEAE
                     QNGTDVDEAIMAAAHG"
     gene            1394..2860
                     /locus_tag="VF874_02720"
     CDS             1394..2860
                     /locus_tag="VF874_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX9175034.1"
                     /translation="MADPAGFLKHRQRELPKRRPIPLRVLDWKEVYEDFDHSELRVQA
                     SRCMDCGIPFCHNGCPLGNLIPEWNDLVRTDRWRDAIDRLHATNNFPEFTGRLCPAPC
                     EASCVLGINQDPVTIKQVEVEIIDNAFEQGWVQPVPPQVLTGKTVAVVGSGPAGLAAA
                     QQLTRAGHAVTVFERDDRIGGLLRYGIPEFKMEKRHVDRRLAQMEAEGTVFKAGVNVG
                     IDITARHLLSDFDAVVLAGGATAWRDLPIPGRDLDGIHQAMEFLPWANRVQEGDDVLG
                     ADGEPPITAKGKKAVIIGGGDTGADCLGTVHRQGATEVHQFEIMPRPPDARAESTPWP
                     TYPLMFRVSAAHEEGGDRVFSVNTEEFIGRDGRVTGLRAHEVQMEGGKFVKVDGTDFE
                     LEADLVLLAMGFVGPERDGLLTELGVEFTDRGNVARGDDYATSVPGVFVAGDAGRGQS
                     LIVWAIAEGRAAAAAVDNYLMGVTALPSPIKPTAAPQR"
     gene            complement(3325..4173)
                     /locus_tag="VF874_02725"
     CDS             complement(3325..4173)
                     /locus_tag="VF874_02725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9175035.1"
                     /translation="MRFAIKTSPQNTTWADMLAVWREADDIDVYESGWTFDHFYPIFS
                     DSTGPCLEGWITLTALAQATQRLRLGTLVTGIHYRHPAVLANMAAALDIVSNGRLELG
                     IGAGWNEEESGAYGIELGTIKERFDRFEEACQVLIGLLSQDTTDFDGRYYQLKAARNE
                     PKGPQRPHPPICIGGSGEKRTLRITARYAQHWNFMGGPPEEFARKRDVLASHCADIGR
                     DPKEIMLSAHVRLGDDLDYAKAVAQAAALGEEGLDLAIIYLPAPHDPAVLEPLANAIR
                     ASELGC"
     gene            4294..4635
                     /locus_tag="VF874_02730"
     CDS             4294..4635
                     /locus_tag="VF874_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lsr2/espR transcriptional regulator"
                     /protein_id="HEX9175036.1"
                     /translation="MKSEPLYKLKGEFFKTLGHPARIRILELLAVRDRSVGELLPEIG
                     VESSNLSQQLGVLRRAGVVTARKDGNTVIYSIASPDIAELLAVARKVLTRMLSDRVAV
                     LEDLRAGVADG"
     gene            4628..5119
                     /locus_tag="VF874_02735"
     CDS             4628..5119
                     /locus_tag="VF874_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX9175037.1"
                     /translation="MGSAVGWVRKILRVGRVAEDSGPGPGLVTNPPAGVRGSLQIRHV
                     DAGSCNGCEVEIGAAFGPVYDAERFGARLVASPRHADALLVTGVVTRNMAGPLCNTVA
                     ATPKPRVVIACGDCALNRGAFEGAYGVVGAVGDVVHVDVQIPGCPPTPAEIVNALRSV
                     TGR"
     gene            5116..7086
                     /locus_tag="VF874_02740"
     CDS             5116..7086
                     /locus_tag="VF874_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9175038.1"
                     /translation="MTATLSTARPAAATTLGHRLGAAVSGAATACVGLTGLWIGLAAI
                     SGARASVRIGWLLPLSGVQLGLDPLGGFFIALTGAVAVPVGVYAIRYAQHLSLGPQIV
                     LPLFVAAMLLVPAAGSVATFLLAWELMAIASLVLVLADHTRVEVRAAGLFYAAMTHLG
                     FVAILVGLMVMSAAAGGDRFADLTHIPDGVRTAVFVLTLAGFGSKAGLVPLHAWLPRA
                     HPEAPSAVSALMSAAMVNLGIYGIVRFDMQLLGPCPPGWALTLLAVGAVSAVYGVLQA
                     SVATDLKRLLAYSTTENMGLVTLALGAAMLLAGSGAASAAAVAMAAATLHLVAHAAFK
                     CLGFMAAGSVLASSGLRDLDQLGGLARRMPATTILFGIAALGASGLPLGAGFISEWLL
                     IQALIHAAPEHNTIVALTTPFAVGAVALTTGLGVAAMVKAFGIGFLARPRSERAEYAR
                     EAPVSMLAGMTIAAAACVVLAVAPVVVATALRRVLDTVPAVRDVGFTDFGAVIRLPGL
                     HGSIAPGVVAAALVVAVLAAVALGHLRARDRPEPVALPLWACGADELTARMQYTATSF
                     AEPLQRVFDDVLRPDTDIEVTHTAESRYMADRVTYRTTISDAIESRLYRPVIGAVAAL
                     AQLVRRAHTASIHLYLAYGALGVLVVLLVVAH"
     gene            7092..8042
                     /locus_tag="VF874_02745"
     CDS             7092..8042
                     /locus_tag="VF874_02745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX9175039.1"
                     /translation="MSYVAGAAQIGMTMMGAPLVIGLMRQVRARLEGRAGGGLLQPWR
                     DIVKQLGKQQITPAGTTALFAAAPAIVAGTALLIAAIAPLVATGSPLDPAADLVAVVG
                     LLFLGTVALTLAAIDSGTSFGGMGASREITIAALVEPTILLAVFALSIPAGSANLGAL
                     VDNTLTHPDQVLSLAGVLAFVALVIVIITETGRLPVDNPATHLELTMVHEAMVLEYAG
                     PRLALVEWAAAMRLTVLLALLSNLFLPWGIAGTHPAAAGVAVGIAAIAVKVTVLAVLL
                     AASEVFIAKLRLFRVPELLAGSFLLALLAVTAANFLTAQA"
     gene            8051..8713
                     /locus_tag="VF874_02750"
     CDS             8051..8713
                     /locus_tag="VF874_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175040.1"
                     /translation="MAANMFGILIDFAAGGLVLAAVLIVWRRDLSAIIGLLAWQGVAL
                     AAIPIVRGGFDGDAALVAVGVGILALRAAVLPWLLARAVGAEQRAHREATPLVNTASS
                     LLIAAVLTVVAFAVTRPVVDLEPDVTTAAVPAAFAVILISLFVMATRRHAVSQAAGFL
                     MLDNGIAAVAFLLTAGVPLIVELGASLDVLFAVIVIGVLTGRLRRTFGEADLDQLTEL
                     RD"
     gene            8713..10182
                     /locus_tag="VF874_02755"
     CDS             8713..10182
                     /locus_tag="VF874_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9175041.1"
                     /translation="MTVLLIVAILAPVLAAVATLIAGWRRPTAMLTVAAATTVLGCGA
                     VLGWRVGGGAHFALGGLLRVDALTVTMLVVIGAVGTLATCAGIGYVDAEIKHGHTDAD
                     SARLYGVLTALFVAAMVFAVCANNIGVTWVAVEATTIVTAFLVGHRRTRAALEATWKY
                     VVICSVGIAIAFLGTVLLYFAAQHAGAPASQALNFDVLLAHAGDLDPAVTRLAAGLLM
                     IGYGAKAGLFPFHTWLADAHSQAPAPVSALMSGVLLSVAFSVLLRIKPIVDIAAGLTF
                     VRAGLVGIGMVTLAIAAAMLTVTPDVKRLLAYSSMENMGLIAIAAAAGTTLAIAALLL
                     HVLAHGIGKTVLFLASGQLQAAHGSTAIRDLGGVMRRSRLIGLSLTVGVLVLLGLPPF
                     GMFASELAIARSVADARMAGVLAIAMLLVAIGFVALARHTGRMLLGAPAAGAPTIAAP
                     TAVAAALLVGIAASAVLGVTAGPLTDLFTTAAGHVGALR"
     gene            10179..11660
                     /locus_tag="VF874_02760"
     CDS             10179..11660
                     /locus_tag="VF874_02760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9175042.1"
                     /translation="MISGRERRRVAGDGLADNAEELLDEGFRIALVAAHDDGDRLRIV
                     YLFLAGRPDRRVELEYSVPPEDPELPSLAYLSFPMSRFEREMADLYGIRPIGHPKPRR
                     LVRHAHWPEDWYPMRESAGPAANFAATGQFPFVTVEGSGVYEIPVGPVHAGLIEPGHF
                     RFSVVGETVLRVKARLWFVHRGIEKLFHGRPADGAIGLAERISGDTSAAHALAHTLAV
                     EDALGIEVPPEMHRLRALAVELERLYNLAADLGALANDVGFGLANCHAQRIRERLLRL
                     NAAVTGHRLLRGAIAPGRVTLHELPDAAALRGIAVDLGEVAELTLANGVVHDRFAGTA
                     ILGSDDARALGCLGYVARASGMRTDARLDHPATELPVSEVGSDAGDVLARYTVRRDEF
                     AASAELACYLVESHAGPTDYYAAAAPAGRETRSGVGIVEGWRGTIVHRVEIDTNGRLT
                     RAKIVDPSWFNWPALPVAMADTIVPDFPLANKSFNLSYAGNDL"
     gene            11721..>12419
                     /locus_tag="VF874_02765"
     CDS             11721..>12419
                     /locus_tag="VF874_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9175043.1"
                     /translation="MDPVAALREIAYYKDRAREESRRVMAYRRAADVVEALDDAERDR
                     LGQTNGWQSLTGVGPKTAKVIAEAWSGQVPELLIELRSGAPDLGGGEIRTALRGDLHL
                     HSNWSDGSARIEEMMLAAQSLGHEYCALTDHSPRLKIANGLSPERLRAQLDVIDELRE
                     KLAPMRILTGIEVDILEDGSLDQEPELLERLDIVVASVHSKLSMDAASMTRRMVRAVS
                     NPHADVLGHCTGRLI"
BASE COUNT         1829 a         4071 c         4338 g         2181 t
ORIGIN      
        1 ggtggcaccg gcgcgacccc gctgacatcg atgaagcatg cgggtgcgcc gtgggagttg
       61 ggtctggccg aaacccagca gacgttgctg ctcaacgggt tacgcgacag gatcgtggtc
      121 caggtggacg gccagctcaa gaccggacgc gacgtgatga tcgccacgct gctgggcgcc
      181 gaggagtacg gtttcgcgac cgcgccgctg gtggtgtcgg gctgcatcat gatgcgggtg
      241 tgccacctcg acacctgccc ggtgggggtg gcaacccaaa accccgagct gcgcaagcgc
      301 tacaacggca agccggagtt cgtcgaaaac ttcttcatgt tcatcgccga agaggcgcgg
      361 gagtacctgg cgcagttggg tttccgcacc atcaacgagg cggtcgggca ggtcggcgcg
      421 ctggacacga cggtggccgc cgagcactgg aaggcccgca agctggacct ttcgccggtg
      481 ctgtacgagc cggagtcggc gttcatgaac caggactact actgcagttc gcgtcaggac
      541 cacgggctgg acaaggcgct cgatcagcag ctgatcgtga tgagccgtga ggcgctggac
      601 gcggaaaagc cggtgaagtt ttctaccacg atctccaacg tcaaccgcac ggtcggcacg
      661 atgctcggcc atgagctcac caaagcctat ggcgggcaag ggcttcccaa cgacaccatc
      721 gacatcacgt tcgacgggtc ggccggcaac agcttcggtg cgttcgtgcc gaagggcatc
      781 acgctgcgcg tctacggcga cgccaacgac tacgtcggca agggactgtc cggcgggcgc
      841 gtcgtcgtgc ggccttcgcg caacgcaccg gacgcctacg tcgccgagga caacatcatc
      901 gcgggcaacg tgatcctgtt cggtgccacc agcggcgagg ccttcctgcg cgggcaggtg
      961 ggcgagcggt tcgcggtgcg caactccggc gcgcacgcgg tggtggaggg cgtcggcgac
     1021 cacggatgcg agtacatgac cggcggcaag gtcgtcatcc tcgggcccac ggggcgcaac
     1081 ttcgcggcgg gcatgtcggg cggtgtggcc tacgtgtacg acccggtgcg tatgctgccc
     1141 gaccatctga actcggagat ggtcgagctc gaggaactcg acgacgacga tttcgaatgg
     1201 ctgtacggga tgctgacgag gcacgtggac gccaccgagt cggcaccggg tacgcggatc
     1261 ctgtcgcact ggccgaccga gcgggcgcgg ttcgtgaagg tgatgccgcg cgactacaag
     1321 cgggtactgg aggcgatcgc ggaggccgag cagaacggga ccgacgtcga cgaggcgatc
     1381 atggcggcgg cgcatggctg accccgccgg ctttctcaaa caccgccagc gcgagttgcc
     1441 caagagaagg cccatcccgc tgcgcgtcct ggactggaaa gaggtctacg aggacttcga
     1501 ccactccgaa ctgcgggttc aggcgtcgcg ctgcatggac tgcggaatcc cgttctgcca
     1561 caacggctgt ccgctgggca atctgattcc cgagtggaac gacctggtgc gcaccgaccg
     1621 ctggcgcgac gcgatcgacc ggctgcacgc caccaacaac ttcccggagt tcaccggccg
     1681 cctttgcccg gcgccgtgtg aggcgtcgtg cgtgctgggc atcaaccagg acccggtgac
     1741 catcaagcag gtcgaggtcg agatcatcga caacgccttc gagcagggct gggtgcagcc
     1801 ggtgccaccg caggtgctga ccggcaagac cgtcgccgtc gtcggttccg gaccggccgg
     1861 cttggcggcg gcgcagcagc tcacccgcgc tgggcatgcg gtgacggtgt tcgagcgcga
     1921 cgaccgcatc ggcggactgc tgcgctacgg catccccgaa ttcaagatgg aaaagcgcca
     1981 cgtcgaccgt cggctcgcgc agatggaggc cgaaggcacg gtcttcaagg ccggtgtgaa
     2041 cgtcggcatt gatataactg cgcgtcacct cctttccgac ttcgacgcgg tcgtgctggc
     2101 gggcggcgcg acggcatggc gcgacctgcc gatcccgggc cgcgacctcg acggcatcca
     2161 tcaggcgatg gagttcctgc cgtgggccaa ccgcgtgcag gagggcgacg acgttctcgg
     2221 tgccgacggg gagccgccga tcaccgcgaa gggcaagaag gctgtcatca tcggcggcgg
     2281 cgacaccggc gcggactgcc tgggcaccgt gcaccggcag ggcgccaccg aggtacacca
     2341 gttcgagatc atgccgcgtc cgccagacgc ccgcgcggag tcgacgccgt ggcccacgta
     2401 cccgctgatg ttccgggtgt cggccgccca cgaagagggc ggcgaccggg tgttctcggt
     2461 caacaccgag gagttcatcg gccgcgacgg ccgcgtgacc gggctgcggg cacacgaggt
     2521 gcagatggag ggcggcaagt tcgtcaaggt cgacggcacc gacttcgagt tggaggccga
     2581 cctggtgctg ctggcgatgg ggttcgtcgg gccggagcgt gacggcctgc tgaccgaact
     2641 cggcgtggag ttcaccgacc gcggcaacgt cgcgcgcggc gacgactacg cgacgagcgt
     2701 gccgggcgtg ttcgtcgccg gcgacgctgg ccgcggccag tcgctgatcg tgtgggccat
     2761 cgccgaaggc cgcgcggcgg cggcggcggt ggacaactac ctgatgggcg tcaccgcgct
     2821 tccgtcgccg atcaagccga cggctgcacc ccagcgttga tgagccgctt gcgcgaagaa
     2881 catcagcgtt gatgagccgc ttgcgcgaag aacatcagca ctgagcgctt gcgcgaagag
     2941 catccagccc cagcggtagc gccggaaaac tctcgacacg cgattgagag tttttggcgc
     3001 gcctcccaaa gtttgccgta aacaaggtgt tcaatgtcta gatgtcggtg gcgcggtgta
     3061 cccttaccac tgcgcaataa tttagagctg gttcaccgca ggagtggtca tcgtgcacct
     3121 caacccgtta ccccgcactc gggtaggtcg aattgcctgg tcatggtttc ggcatcccgt
     3181 gaagagccgc gagttcccag ccaagcacga gcgcctggtg accgcagccg agctactgcg
     3241 ctacagcgtc tgacacagcc gcccggcggc gtaccgtcat cgcgcggcca cgattcaact
     3301 ttgctggcct ttatcaatcc gttatcaaca gcccaattct gaggccctga tcgcattcgc
     3361 gagaggctcc aacacggccg gatcgtgggg cgcaggcaga tagatgatcg cgaggtcgag
     3421 cccttcctcg cccaacgccg ccgcctgcgc gaccgccttg gcgtagtcga gatcgtcacc
     3481 gaggcggacg tgcgcggaca gcatgatctc cttcgggtcg cggccgatgt cggcgcagtg
     3541 cgacgcgagc acgtcacgct tccgggcgaa ctcctccggc ggcccgccca tgaagttcca
     3601 gtgctgggcg tacctcgcgg tgatgcgcag tgtccgcttc tcgccgctgc cgccgatgca
     3661 gatcggcgga tgcggtcgtt gcgggccctt cggctcgttg cgtgcggctt tgagctggta
     3721 gtaccgcccg tcgaagtcgg tggtgtcctg ggacagcaga ccgatcagca cctggcacgc
     3781 ctcttcgaag cggtcgaacc gctccttgat cgtcccgagt tcgatcccgt atgcaccgga
     3841 ctcctcttcg ttccagcctg cgccgatgcc gagttcgagc ctgccgttgg agacgatgtc
     3901 caacgccgcg gccatgttgg ccagcacggc gggatgccgg tagtggatgc cggtgaccag
     3961 tgtgcccagt cgtagccgct gcgtggcctg cgccagcgcc gtcagagtga tccagccctc
     4021 caggcacggc cccgtggagt cggagaagat cggatagaag tggtcgaacg tccagccgga
     4081 ctcgtacacg tcgatgtcgt cggcctctcg ccagacggcg agcatgtcgg cccaggtggt
     4141 gttctgcggt gaggtcttga tcgcgaatcg cactccttcc acgttaggcg ccattcctga
     4201 atgtgcaggg gcaaccacac cagccgtaaa cgcactatct gataagttct tcaacaaatc
     4261 aatcgatgga acagcaaagt cttcaggaga acggtgaagt cggagccgct gtacaagctc
     4321 aagggcgagt tcttcaagac gctcgggcat ccggcccgaa tccggatcct cgaactgttg
     4381 gcggtgcgtg accggtcggt cggcgagctc ctgcccgaga tcggagtcga gtcgtcgaat
     4441 ttgtcgcaac agttgggcgt gctgaggcgg gcgggggttg tcaccgcacg caaggacggc
     4501 aatacggtga tctactcgat cgcctcgccg gacatcgccg aacttctggc cgtggctcgc
     4561 aaggttttga ccaggatgct cagcgatcgc gtcgcagtgc tcgaggatct gcgcgccggc
     4621 gttgccgatg ggtagcgccg tcggctgggt ccgtaagatt ctgcgagtcg gtcgcgtcgc
     4681 ggaggacagc ggcccgggac ccggcctggt caccaatccg cctgccggag tgcggggttc
     4741 actgcagatc cgccatgtcg acgccggatc gtgcaacggc tgtgaagtgg agatcggggc
     4801 cgccttcggc cccgtgtatg acgccgagcg gttcggtgcg cgactcgtgg cgtcgccgcg
     4861 gcatgccgac gccctgctgg tcaccggcgt ggtgacgcgc aatatggccg ggccactgtg
     4921 caacacagtt gcggcgaccc cgaaaccgcg cgtggtgatc gcatgcggcg actgtgcgct
     4981 gaaccggggc gccttcgagg gcgcctacgg cgtggtgggt gcggtcggtg acgtcgtgca
     5041 cgtcgacgtg cagatcccgg gatgcccgcc cacgccggcc gagatcgtca atgcgttgcg
     5101 gtcggtcacg ggacgatgac cgccacgctt tcgaccgcgc gacctgcggc ggcgacgacg
     5161 ctcggccacc gtcttggcgc ggccgtctcc ggtgccgcga ctgcctgtgt cggtctgacc
     5221 gggttgtgga ttggcctagc cgccatctcc ggcgcgaggg cgagtgtgcg catcggctgg
     5281 ttgctaccgc tgtccggggt gcaactgggt ctggatccgc tcggtggctt cttcatcgcg
     5341 ctcaccggcg cggtggccgt tccggtcggt gtctacgcga tccgctacgc acaacatctt
     5401 tccctgggac cgcagattgt gctgccgttg ttcgtcgcgg cgatgcttct cgtgcctgcc
     5461 gcaggttcgg tggcgacgtt cctgctggcg tgggaactca tggcgatcgc gtcactggta
     5521 ctggtgctcg ccgaccacac acgggtagag gtccgcgccg ccgggctgtt ctacgccgcg
     5581 atgactcacc tcgggtttgt cgcgattctg gtcggtttga tggtgatgtc ggctgccgca
     5641 ggcggggacc ggttcgcgga cctgacccac atacccgacg gtgtgcggac cgcggttttt
     5701 gtgctcacgc tggccggatt cgggtcgaag gccggcctgg tgccgctgca cgcctggctg
     5761 ccgcgcgccc atccggaggc gcccagcgcg gtgtcggcgc tgatgagcgc cgcgatggtg
     5821 aacctcggca tctacggcat cgtccggttc gacatgcagc tcctcggccc ttgcccgccc
     5881 gggtgggcgc tcaccctgtt ggcggtgggc gccgtctcgg cggtctacgg cgtgctgcag
     5941 gcatcggtgg cgacggatct caaacggctg cttgcgtatt cgacaaccga gaacatggga
     6001 ctggtcacgc ttgcgcttgg cgccgcgatg ctgcttgcgg ggtccggcgc cgcctctgct
     6061 gccgcagtcg cgatggccgc ggcgacgctg cacctggtcg cgcacgcggc cttcaagtgc
     6121 ctcgggttca tggccgcagg gtcggtactc gcgtcgtcgg ggctgcgcga tctcgaccag
     6181 ttgggcgggc tggcccggcg aatgccggcc acgacgattc tcttcggtat cgccgcgctg
     6241 ggtgcgtcgg gtctgcccct cggggccggg ttcatcagcg agtggctact gatccaggca
     6301 ctgatccatg ccgcacccga acacaacacg atcgtcgcgt tgacaacacc gttcgcggtc
     6361 ggcgcggtcg cactcaccac cggcctcggt gtggcggcca tggtcaaggc gttcgggatc
     6421 ggattcctgg cccggccgcg gtccgagcgg gccgagtacg cgcgcgaggc gccggtgagc
     6481 atgctcgccg ggatgacgat cgcggcggct gcgtgcgtgg tgctcgcggt ggctccggtc
     6541 gtggtcgcga cggcgctgcg acgtgtgctc gacaccgtgc ccgcagtccg tgacgtcggc
     6601 ttcacggact tcggggcggt gatacggctg ccggggctgc acggctcgat cgcccccggt
     6661 gtggtcgccg cggctctcgt ggtcgccgtg ctggccgctg ttgccctggg tcacctgcgc
     6721 gcacgcgacc ggcccgaacc ggtggcgctg ccgctgtggg cttgcggcgc cgacgagctg
     6781 accgcgcgga tgcagtacac cgcgacgtcg ttcgccgagc cgctgcagcg cgtgttcgac
     6841 gacgttctgc gccctgacac cgacatcgag gtcactcaca ccgccgaatc gcggtacatg
     6901 gccgaccggg tcacctaccg gaccacgatc agcgacgcga tagagagccg gctttaccgc
     6961 ccggtcatcg gagccgtcgc ggccctggcg cagctggtgc gccgggcgca cacggcgagc
     7021 attcacctgt acctcgccta tggtgcgctc ggcgtcctgg tcgtgctgct ggtggtggcg
     7081 cattgaacgt catgtcgtac gtcgcgggtg ccgcacagat cggtatgacg atgatgggcg
     7141 cacccctggt gatcgggctg atgcgccagg tgcgcgcccg gttggagggc cgcgccgggg
     7201 gtgggctact gcaaccgtgg cgtgacatcg tcaaacagct tggcaaacaa cagattacgc
     7261 ccgccggaac gacggcgctg ttcgctgcgg ccccggcgat cgtcgccggc accgcgctgc
     7321 tcatcgccgc gatcgcgccg ctggtcgcaa ccggatcgcc gctcgatccg gccgccgacc
     7381 tcgtcgctgt cgtcggcttg ctcttcctgg gcaccgtggc gttgacgctg gcggcaatcg
     7441 acagcggaac atcgttcggc ggcatgggcg cgagccggga gatcaccatt gcggcgctcg
     7501 tcgaaccgac gatcctgctc gccgttttcg cattgtccat tccggctgga tcagcgaatc
     7561 tgggtgcgct ggtggataac acgctcacgc atcccgacca ggtgctgtcc ctggcaggcg
     7621 tactcgcatt cgtcgcgctg gtgatcgtca tcatcaccga gaccggccgg ctgccggtgg
     7681 acaaccccgc tacgcatctg gagttgacca tggtgcacga ggccatggtg ctcgagtacg
     7741 ccggcccgcg actcgcgctg gtggagtggg cggcggcgat gcgactgacc gtcctacttg
     7801 cgctgctgtc gaacttgttc ttgccgtggg ggatcgccgg cacgcatccg gcggcggccg
     7861 gcgtcgcggt cggcatcgcg gcgatcgctg tcaaggtgac ggtgctggcc gtgctgctgg
     7921 ccgcctccga ggtgttcatc gccaagctga ggctgttccg ggttcctgag ctgctcgcgg
     7981 gttcgtttct gctggcattg ctcgccgtca ccgcagccaa cttcctcacg gcgcaggctt
     8041 agggaagccc atggcggcca acatgttcgg gattctcatc gacttcgccg ccggcgggct
     8101 cgtactcgct gccgtgctga tcgtgtggcg cagggacttg tccgcaatca ttgggctgct
     8161 ggcgtggcag ggcgtggcgc tggcggcgat cccgatcgtg cgcggcggct tcgacggcga
     8221 cgccgcgctg gtcgccgtcg gcgtcggcat tctcgctctg cgcgcggcgg tattgccgtg
     8281 gctgctggcc cgcgcggtcg gtgccgaaca gcgggcgcac cgagaggcca cgcccctggt
     8341 caacaccgct agttcactgc tgatcgccgc cgtgctgacc gtcgtcgcgt tcgcggtcac
     8401 ccgcccggtg gtcgacctgg agccggacgt gacgaccgcg gcggttccgg cggcgttcgc
     8461 ggtgatcctc atctcgctgt tcgtgatggc cacccgccgg cacgcggtgt cgcaggcggc
     8521 cggatttctg atgctcgaca acgggatcgc cgcggtcgcg ttcctgctga cagcgggggt
     8581 accgctgatc gtcgagctgg gtgcgtcgct ggacgtgctg ttcgcggtga tcgtcatcgg
     8641 cgttctcacc ggccgcctgc ggcgcacgtt cggtgaggcg gatctggatc agttgacgga
     8701 gttgcgggac tgatgactgt gttgctgatc gttgcgatcc tcgcgcccgt gctcgcggcg
     8761 gtcgcgacgc tgattgcggg ctggcgccgc ccgaccgcga tgctgacggt ggcggccgcg
     8821 acgacggtcc tcgggtgcgg cgcggtcctt ggctggcggg tgggcggcgg cgcgcacttc
     8881 gccctgggcg ggctgctgcg tgtcgacgca ttgaccgtga ccatgctcgt cgtcatcggc
     8941 gctgtcggca cacttgccac ctgcgccggc atcggctacg tcgacgccga gatcaagcat
     9001 ggccacaccg acgccgacag cgcccggctc tacggcgtgc tgacggcgct gtttgtggcg
     9061 gcgatggttt tcgcggtgtg cgccaacaat atcggagtga catgggtggc ggtcgaggcg
     9121 acgaccatcg tgacagcctt cctggtcggg caccgccgga cccgcgcggc gttggaggcg
     9181 acgtggaagt acgtcgtgat ctgttcggtc ggcattgcga tcgcattcct cggcaccgtg
     9241 ctgctttact tcgccgccca gcacgccggc gcgccggcca gccaggcgct gaacttcgac
     9301 gttctactcg cccacgccgg ggacctcgac ccggcggtga cccggctcgc cgccgggttg
     9361 ctgatgattg gatacggcgc caaggcgggt ctgttcccgt tccacacctg gctggcggac
     9421 gcccacagcc aggcgccggc gccggtgtcg gcgctgatga gtggtgtgct gctgtcggtg
     9481 gcgttctccg tgctgctgcg gatcaagccg atcgtcgaca tcgccgccgg actgacgttt
     9541 gtgcgtgccg gactggtggg gatcgggatg gtgacgcttg cgatcgcggc agccatgctg
     9601 accgtcacgc ccgatgtcaa acggctgctt gcctattcgt cgatggagaa catgggtctg
     9661 atcgcgatcg ccgcggcagc tgggaccact ctggcgatcg ccgcgttgct gctgcatgtg
     9721 ctggcacacg ggatcggcaa gaccgtcctg ttcctggcga gtggtcagct gcaggccgcg
     9781 cacggctcca ccgcgatcag agacctcggc ggggtcatgc ggcggtcccg gctgattggc
     9841 ctctcgttga ccgtcggcgt actcgtcctt cttgggctgc cgcctttcgg aatgttcgcc
     9901 agcgaactgg cgatagcccg ttcggtcgcg gacgcccgga tggcgggtgt gcttgcgatt
     9961 gcgatgctgc tcgtcgcgat cgggtttgtc gcactggcgc gccataccgg ccggatgctg
    10021 ctcggcgcgc cggcggccgg cgcaccgacg atcgccgcgc ccaccgccgt cgcggcggcc
    10081 ctgctggtgg gtatcgccgc gtcggcggtg ctcggtgtca ccgcgggccc actgaccgat
    10141 ctgttcacga cggccgcggg ccacgtcggg gcgttgcgat gatctccggc cgggaacgtc
    10201 gtcgggtggc tggagacggg ctggccgaca acgcagaaga gttgctggac gagggttttc
    10261 gcattgcgct ggtcgccgct cacgacgacg gcgaccgact gcggattgtg tatctgttcc
    10321 tggccggccg accagaccgc agggtggaac tcgaatactc ggtgccccca gaggatcccg
    10381 agctgccgtc gctggcttac ctgtcgttcc cgatgagccg cttcgaacgc gaaatggccg
    10441 acttgtacgg cattcggccc atcggccatc ccaagccgcg ccgactggtc cggcacgcgc
    10501 actggcccga agactggtat ccgatgcggg agagtgcagg gccggctgcg aacttcgcgg
    10561 ccactggtca attcccgttt gtcaccgtcg agggatccgg ggtgtacgaa attcccgtcg
    10621 gtccggtgca cgcgggcctg atcgagccgg gccacttccg gttttccgtc gtcggcgaga
    10681 ctgtgctgcg ggtgaaggca cggctgtggt tcgtccaccg cggcatcgag aaactcttcc
    10741 acgggcgacc agccgacggc gcgatcgggc tcgccgagcg catcagcggc gacacctctg
    10801 ccgcgcatgc tctcgcccac accctggccg tcgaggacgc gctcggcatc gaggttccgc
    10861 ccgagatgca ccggctgcgc gcgctcgcgg tcgaactcga acggctctac aacctggccg
    10921 ccgacctcgg tgcgctggcc aacgacgtcg gattcgggct cgccaattgt catgcgcaac
    10981 gcatacgtga acgacttctg cgcctcaatg ccgccgtaac cggccatcgg ctgctgcgcg
    11041 gggcgatcgc tcccggcagg gtaacgctgc acgagcttcc agatgccgca gcgctgcgcg
    11101 gcatcgccgt cgacctcggc gaggtcgccg agctgaccct tgccaacggc gtcgtccacg
    11161 accgctttgc tggcacggcg attctcggct ccgacgatgc gcgggcgctc ggctgcctgg
    11221 ggtacgtggc ccgcgccagc ggcatgcgca ccgacgcccg gctcgaccat cccgctaccg
    11281 aattacccgt cagcgaggtt ggatccgatg ccggtgacgt gctggcccgc tacaccgtgc
    11341 ggcgtgacga gttcgctgcc tccgcggagc tcgcctgcta tctcgtcgag tcacacgccg
    11401 gccccaccga ctactacgcc gccgcggcgc ctgctggccg cgaaacccga agcggcgtcg
    11461 gcatcgtcga gggctggcgc ggcacgatcg tgcaccgcgt cgaaatcgac accaacggtc
    11521 gcctcacccg cgcgaaaatc gtcgacccgt cctggttcaa ctggcccgcg ctcccagtcg
    11581 ccatggccga cacgatcgtc cccgatttcc cgctggccaa taagagcttc aacctctcct
    11641 acgccggcaa tgacctgtag aacccgcgga cggcccgcgg tcgccgaaag ggcggtcgcg
    11701 ggtgagggca gactgaaccc atggacccgg tggccgcgct gcgtgaaatc gcgtactaca
    11761 aggaccgcgc tcgcgaagag tcgcggcgcg tgatggccta ccgacgcgcg gccgacgtcg
    11821 tggaggcgct cgacgacgcc gaacgggacc ggctgggcca aaccaacggc tggcagagcc
    11881 tgactggggt ggggccgaag accgcgaagg tgatcgccga ggcgtggtcg ggtcaggtac
    11941 cggagcttct catcgaatta cgttcgggtg caccggattt gggcggcggt gagatccgca
    12001 ccgctctgcg cggtgatctg cacctgcatt cgaactggtc agacggctcg gcccggatcg
    12061 aggagatgat gcttgctgcc cagtcgctcg ggcacgaata ctgtgcgctc accgaccact
    12121 cgccgcggct gaagatcgcc aacggcttgt ccccggagcg gctgcgggcg cagctggacg
    12181 tgatcgacga gctgcgagag aagctggcgc cgatgcggat tctgacgggt atcgaggtcg
    12241 acatcctcga ggacggctcc ctggatcagg aacctgagct gctcgagcgg ctcgacatcg
    12301 tggtggccag cgtgcactcc aagctgtcga tggacgctgc atccatgacg cgccggatgg
    12361 tgcgcgcggt gtcgaacccg catgccgacg tgctcgggca ctgcaccggg cggctcatc
//
LOCUS       DASWBF010000087         5234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_268064,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000087 DASWBF010000000
VERSION     DASWBF010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268064"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2016
                     /gene="infB"
                     /locus_tag="VF874_02770"
     CDS             <1..2016
                     /gene="infB"
                     /locus_tag="VF874_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX9175044.1"
                     /translation="GGAPGGGAPGGAPTTGGFRGRPGGGGRPGQRGGAAGAFGRPGGA
                     PKRGRKSKRQKRQEYDSMQAPVVGGVRLPHGNGETIRLARGASLSDFAEKIDANPAAL
                     VQALFNLGEMVTATQSVGDETLELLGGEMNYNVQVVSPEDEDRELLESFDLTYGEDEG
                     SEEDLQTRPPVVTVMGHVDHGKTRLLDTIRQANVREGEAGGITQHIGAYQVSVEHDGV
                     ERPITFIDTPGHEAFTAMRARGAKATDIAILVVAADDGVMPQTVEAINHAQAADVPIV
                     VAVNKIDKEGADPSKVRGQLTEYGLISEDFGGDTMFVDISAKQGTNIEQLLEAVLLTA
                     DAALDLRANPEMEAQGVAIEAHLDRGRGPVATVLVQRGTLRVGDSVVAGDAYGRVRRM
                     VDEHGEDVEEALPSRPVQVIGFTSVPGAGDNLLVVDEDRIARQIADRRSARKRNAMAA
                     RARKRISLEDLDSALKETSQLNLILKGDNAGTVEALEEALLGIQVDDEVELRVIDRGV
                     GGVTETNVNLASASDAIIIGFNVRAEGKATELANREGVEIRYYSVIYQAIDEIEKALR
                     GLLKPIYEERELGRAEVRALFRSSKVGTIAGCLVTSGIIRRNAKARLLRDSVVVSENM
                     TVQSLRREKDDVTEVRDGYECGLTLSYSDIKEGDVIETYELVEKERA"
     gene            2082..2576
                     /gene="rbfA"
                     /locus_tag="VF874_02775"
     CDS             2082..2576
                     /gene="rbfA"
                     /locus_tag="VF874_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX9175045.1"
                     /translation="MSDPARARRLAKRISTIVASAIEYEIKDPGLAGVTITDAKVTAD
                     LHDATLYYTVLGRTLEDAPDYAGAAAALERAKGVLRTKVGAGTGVRFTPTLTFVRDTV
                     PDSAHRMEELLARAREADADLARARRGAKPAGDPQPYRVVGDNGDATTTDGDQGSGDG
                     DRND"
     gene            2554..3549
                     /locus_tag="VF874_02780"
     CDS             2554..3549
                     /locus_tag="VF874_02780"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX9175046.1"
                     /translation="MTAIETTEEEAVETRIDARGAAKALADARTVSIVCHVHPDADSV
                     GAGLALALVLDRDGVDVEIGFATPDRLPESLRTLPGCQFLVPPQDMRRDADLVVTVDA
                     PSTARLGALADLASPGNDVLVIDHHKSNRLFGSLNFVDASADSTTMLVAELLDTWGKE
                     IDRDVATCLYAGLTIDTGSFRWASPGALRLAARLVEAGADNAAISRELLDTHPYAWLP
                     MLSRVLATAQLLPDTVGGRGLVYVVVSHDEWMGSRSEEVESIVDIVRTTQQAEVAVVF
                     KEIEPQQWSVSMRAKSLDLASVATQFGGGGHQLAAGYSASGPIDDVVAALLDAVG"
     gene            complement(3756..4082)
                     /locus_tag="VF874_02785"
     CDS             complement(3756..4082)
                     /locus_tag="VF874_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175047.1"
                     /translation="MRAAVDDLFRAGQGLGWGITATGVLVETLLDRAAEGDVTEARNA
                     IDRLVAAPGDEELALREIWLLRMRALLARAHGDDEAYRELKERYRDMATSLDFEGHMA
                     WAEMMP"
     gene            complement(4082..4444)
                     /locus_tag="VF874_02790"
     CDS             complement(4082..4444)
                     /locus_tag="VF874_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175048.1"
                     /translation="MAIARATDAVSHAFVINGKYGPSITNGLLLADDFALREIEEALR
                     IAEGSVDNIALGNARLVYGFALVHQDCADRERSLEILADVRGMCLHDRFHLSELPTID
                     VFMARERARRGDWKVPCR"
     gene            complement(4563..>5234)
                     /locus_tag="VF874_02795"
     CDS             complement(4563..>5234)
                     /locus_tag="VF874_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HEX9175049.1"
                     /translation="DQVKFTPRAEYAFRHPLIRTVAYASQLKADRAELHRRLASAIEA
                     RTSTDEDAALIAEHLEAAGDLGEAYAWHMRAGAWSTSRDIVAAVLSWQRARQIADSLS
                     ADHPDRLALRIAPRTRLCANGWRVGDWSSGTRFEELQQLCMEAGDKASLAMGMAGLLM
                     ELMFQARVREASQLASEYMALLESIGDSTLTGRAFVRSDLREGSGRRDGRCAAVGTDR
                     HRSRE"
BASE COUNT          925 a         1749 c         1781 g          779 t
ORIGIN      
        1 gggggcgccc ccggcggtgg cgcccccggc ggcgcgccca caaccggcgg cttccgtggc
       61 cgtcccggcg gcggcggtcg ccccgggcag cgcggtggag cggccggcgc gttcggccgt
      121 cccggcggcg cgcccaagcg cggccgcaag tccaagcggc agaagcgcca ggaatacgac
      181 tcgatgcagg caccggtcgt cggcggcgtg cggctgccgc acggcaacgg cgagaccatc
      241 cggctggccc gcggcgcgtc gctgtctgac ttcgcggaga agatcgacgc caacccggcg
      301 gctctcgtgc aggcgttgtt caacctcggc gagatggtca ccgccaccca gtccgtcggc
      361 gacgagacgc tcgagctgct cggcggcgag atgaactaca acgtccaagt cgtcagcccc
      421 gaggacgagg accgcgagct gctggagtcc ttcgacctga cctacggcga ggacgagggc
      481 agcgaggagg acctgcagac tcgaccgccg gtggtcaccg tgatgggcca cgtcgaccac
      541 ggcaagacgc gcctgctgga caccatccgc caggccaacg tccgcgaggg cgaggccggc
      601 ggcatcactc agcacatcgg cgcctaccag gtctccgtcg agcacgacgg cgtcgaacgg
      661 ccgatcacgt tcatcgacac cccgggccac gaagcgttca ccgcaatgcg ggcccgcggc
      721 gcgaaggcca ccgacatcgc gatcctcgtg gtggccgccg acgacggtgt gatgccgcag
      781 acggtggaag ccatcaacca cgcgcaggcc gccgacgtgc cgatcgtggt ggcggtaaac
      841 aagatcgaca aagagggcgc cgacccgtcc aaggttcgcg gccagctgac cgaatacggg
      901 ctgatttccg aagatttcgg cggcgacacc atgttcgtcg acatctccgc caagcagggc
      961 accaatatcg agcagctgct cgaggcggtg ctgctgaccg ccgacgccgc gctggacctg
     1021 cgggccaacc ccgagatgga ggcgcagggc gtggcgatcg aggcgcatct ggaccgcggc
     1081 cgcgggccgg tggccaccgt gctggtgcag cgcggcacgc tgcgcgtcgg cgactcggtg
     1141 gtggccggcg acgcgtacgg ccgggtgcgc cgcatggtcg acgagcacgg cgaggacgtc
     1201 gaggaggcgc tgccgtcgcg gccggtgcag gtcatcggct tcacgtcggt gcccggcgcg
     1261 ggtgacaacc tcctcgttgt cgacgaggac cgcatcgccc gccagatcgc cgaccggcgc
     1321 agcgcgcgca agcgcaacgc gatggcggcc cgggcccgca agcggatcag cctggaggac
     1381 ctggattcag cgctgaagga aaccagccaa ctgaacctga tcctcaaggg cgacaacgcc
     1441 ggcacggtgg aggcgctgga agaggcgctg ctgggcatcc aggtcgacga cgaagtcgag
     1501 ctgcgcgtca tcgaccgcgg agtcggcggc gtcaccgaaa ccaacgtcaa cctggcgtcg
     1561 gccagcgacg cgatcatcat cggcttcaac gtgcgcgccg agggcaaggc caccgagctg
     1621 gccaaccgcg agggtgtgga gatccgctac tactcggtga tctaccaggc catcgacgag
     1681 atcgagaagg cgctgcgcgg tctgctcaag ccgatctacg aggaacgcga gctgggccgg
     1741 gccgaggtgc gggcgctgtt ccgcagctcg aaggtcggga ccatcgccgg ctgccttgtc
     1801 acgtcgggca tcatccggcg caacgcgaag gcgcggctgc tgcgcgacag cgtggtggtg
     1861 tccgagaaca tgaccgtgca gtcgctgcgc cgggagaagg acgacgtcac cgaggtccgt
     1921 gacggctacg agtgcggtct gacgctgtcg tattccgaca tcaaggaagg cgacgtgatc
     1981 gagacgtacg agctcgtcga gaaggaacgc gcgtaagtgg gcgatgagcg ctcgcgctgc
     2041 gatgagcctc tcgggcgaag agccacgaac agagagcaga catgagcgat ccagcacgag
     2101 cgcgtcggct ggccaagcgg atttcgacga tcgtcgcgtc ggcgatcgaa tacgagatca
     2161 aggatccggg gctggccggc gtgacgatca ccgacgcgaa ggtcaccgcg gacctgcacg
     2221 acgcgacgct gtactacacg gtgttgggcc gcaccctcga ggacgcgccg gactacgccg
     2281 gcgcggcggc cgcgctggag cgggccaagg gcgtgctgcg cacgaaagtc ggtgcgggaa
     2341 ccggtgttcg gttcacaccg acgctgacgt tcgtgcgcga caccgtgccg gattccgccc
     2401 accggatgga ggagctgctg gcgcgcgccc gtgaagcgga cgcggatctg gcgagggcaa
     2461 ggcggggtgc caaacctgcg ggcgacccgc agccgtaccg tgtggtagga gataacggag
     2521 acgcgacgac gacagacggc gaccagggca gcggtgacgg cgatcgaaac gactgaagaa
     2581 gaagcggtcg agacccgcat tgacgcgcgg ggcgcggcga aggcgctcgc cgacgcccgc
     2641 acggtcagca tcgtctgcca cgttcacccc gacgccgaca gcgtcggcgc gggtctggcc
     2701 ttggcgctgg tgctcgaccg ggacggcgtc gacgtggaaa tcggtttcgc aacgccggat
     2761 cggctgccgg agtcgctgcg gacgctgccc ggctgccagt tcctggtgcc accccaggac
     2821 atgcgccgcg acgccgatct ggtggtgacc gtcgacgcac cgagcaccgc ccggctgggg
     2881 gcgctggcgg atctggcatc gccgggcaac gacgtgctgg tgatcgacca ccacaagtcc
     2941 aatcgcttgt tcggcagcct gaatttcgtt gacgcgtcgg ccgattcgac gaccatgctg
     3001 gtcgccgaac tccttgatac ctggggcaaa gagatcgacc gtgatgtcgc cacctgcctg
     3061 tacgcaggat tgaccattga caccgggtcg tttcggtggg ccagcccggg ggcgctgcgc
     3121 ctggcagcgc ggctggtcga agccggtgcg gacaacgccg cgatctcccg cgagctcctc
     3181 gacacgcatc cgtacgcgtg gctgcccatg ctgtcgcggg tgttggcgac ggcgcagctg
     3241 ctgcctgaca ccgtgggcgg gcgtgggctg gtctacgtcg tcgtctccca cgacgagtgg
     3301 atgggcagcc ggtccgaaga ggtcgaaagc atcgtcgaca tcgtccgaac cacgcagcag
     3361 gccgaggtcg cggtggtgtt caaggagatc gagccgcagc agtggtcggt gtcgatgcga
     3421 gccaagtctt tggatctcgc ttctgtcgca acgcaattcg gtggcggcgg tcatcagctc
     3481 gcggccggct actccgcgtc gggtccgatc gacgacgtgg tggcggcgct gctggacgcg
     3541 gttggctgac aacccggcaa agcaattctt cgcatcggcg tcctcggcgc catgtcaaac
     3601 aatgcttcga acgctaattt cggccatacg caatttgaga cggactcttg agacgcggcc
     3661 acctcgcggt tttcttcgac agtaggagat ggcttcggct gtctcatatg tcgtctattt
     3721 cgagacgcgg gcatcgccct ccggtccgac cgccgtcatg gcatcatctc ggcccacgcc
     3781 atatgcccct cgaaatcgag cgacgtcgcc atgtcgcggt agcgctcctt gagctcgcgg
     3841 taggcctcgt cgtcgccgtg ggcgcgagcc agcagcgcac gcattcgaag cagccagatc
     3901 tcgcgcagcg caagttcttc atcgccggga gcggccacca accggtcgat agcgttccgg
     3961 gcttcggtga cgtcaccttc ggcggcgcgg tccagaagcg tctccacgag cacaccggtg
     4021 gccgtaatgc cccacccaag cccctggccc gcgcgaaaca aatcgtccac ggctgctcgc
     4081 atcagcggca cggcaccttc cagtcgcccc gcctggccct ctcccgcgcc atgaacacat
     4141 cgatggtcgg caattcgctc aggtggaacc tgtcatgaag gcacatgccg cggacatccg
     4201 ccaatatctc caagctgcgc tcgcggtccg cgcagtcttg atggaccagt gcaaagccgt
     4261 acaccaatct ggcgttgccg agcgcgatat tgtctaccga tccctcggcg atccgcagcg
     4321 cttcctcgat ctcgcgcagc gcgaagtcgt ccgccagcaa caacccgttc gttatcgacg
     4381 gaccgtactt gccgttgatc acgaaagcgt gtgacacggc gtcggtcgcg cgggcgatcg
     4441 ccacggcgcg gtcgaagtcg tctcgccacc caggcagacc gatccatgat cggacgatgc
     4501 cgcgcaacac caacgtcact gccaatggcg accaaccaag gaagttgccc ttgatggggt
     4561 cgtcactcgc gagaccgatg acggtctgtg cccaccgcag cgcatcggcc atctcgccgg
     4621 cctgagcctt cgcgaagatc gctccgaacg aaagcccgac cggtcaatgt cgaatccccg
     4681 atcgactcga gcagggccat gtattcggat gccagctgcg acgcttcgcg cacacgagct
     4741 tggaacatga gctccatcag caacccagcc atgcccatag ccagtgacgc cttgtccccg
     4801 gcttccatgc agagctgctg gagctcctcg aatcgggtcc cagaactcca atcaccgact
     4861 cgccagccgt tggcgcacaa ccgggtacgg ggagcgatgc gcaacgccag ccggtccggg
     4921 tgatcggcgg acagtgagtc ggcaatctgg cgtgcacgct gccagctcag aaccgccgct
     4981 acgatatcgc ggctggtcga ccacgcgcca gcgcgcatgt gccacgcata cgcctccccc
     5041 aggtcacccg cggcttcgag atgctcggcg atgagcgccg cgtcctcatc ggtcgaggtc
     5101 cgcgcttcga tcgcactggc cagccgccgg tgcagctctg cgcgatcggc cttgagctgc
     5161 gacgcatagg ccaccgtgcg gattaatggg tggcgaaacg cgtactcggc gcgaggggtg
     5221 aacttcacct gatc
//
LOCUS       DASWBF010000088         9450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_72256, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000088 DASWBF010000000
VERSION     DASWBF010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72256"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VF874_02800"
     CDS             <1..266
                     /locus_tag="VF874_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX9175050.1"
                     /translation="PGYLSEVHHVNEWAVHHRTDIDDLTFACGPCHKLLDDDGWTTRK
                     NADGVTEWIPPPHLDFGKPRTNGYWHPQRYFRLDDNVDDGDPP"
     gene            497..862
                     /gene="trxA"
                     /locus_tag="VF874_02805"
     CDS             497..862
                     /gene="trxA"
                     /locus_tag="VF874_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9175051.1"
                     /translation="MATQDITTDQFNETINDNEVVLVDFWASWCGPCKQFAPTFEASS
                     KKHPNVVYAKVDTEAEQELAARAQIRAIPTLMAFKKGTLVFNQAGALTPPMLEDLVQQ
                     IEALDVEAALAAQNDGEQA"
     gene            881..1704
                     /locus_tag="VF874_02810"
                     /pseudo
     CDS             881..1704
                     /locus_tag="VF874_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
     gene            1717..3345
                     /locus_tag="VF874_02815"
     CDS             1717..3345
                     /locus_tag="VF874_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9175052.1"
                     /translation="MITATNLEVRAGARILLSAAGSALRVQPGDRIGLVGRNGAGKTT
                     TMRILAGDAEPYAGTIHRKGEIGYLPQDPKEGNLEVIARDRVLSARGLDTLMADLEKQ
                     QALMAEVVDDIERDKAVRRYGQLEERFAALGGYVAESEAARICASLGLPDRVLTQPLR
                     TLSGGQRRRVELARILFAASDTGAGSDTTLLLDEPTNHLDADSLGWLRDFLQNHAGGL
                     VVISHNVDLLADVVNKVWFLDAVRSEIDVYNMGWHKYLDARATDEQRRRRERANAERK
                     AAALRTQAAKLGAKATKAVAAQNMLRRADRMMAALDDERVADKVARIKFPTPAPCGRT
                     PLTAKGLTKNYGSLEVFSGVDLAIDRGSRVVVLGLNGAGKTTLLRLLAGVETPDTGVL
                     EPGHGCRIGYFAQEHDTLDNMATVWENIRHAAPDSGEQDLRGLLGAFMFSGPQLDQRA
                     GTLSGGEKTRLALAGLVASTANVLLLDEPTNNLDPASREQVLDALRSYCGAVVLVTHD
                     PGAAEALAPQRVVLLPDGTEDYWSEDYRDLIELA"
     gene            3435..3629
                     /locus_tag="VF874_02820"
     CDS             3435..3629
                     /locus_tag="VF874_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9175053.1"
                     /translation="MRNPDKGREDLLHQLRDQYEHGASIRKLVASTGRSYGSIHSMLR
                     ESGTTMRGRGGPNHTRRRAS"
     gene            complement(3657..4220)
                     /locus_tag="VF874_02825"
     CDS             complement(3657..4220)
                     /locus_tag="VF874_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9175054.1"
                     /translation="MPRVSEEHLAARRRQILDGARRCFAQHGYDRATVRRLEQSIGLS
                     RGAIFHHFRDKDALFFELAREDAERMADVASREGLIQVMREMLANPDQFDWLATRLEI
                     ARKLRNDPAFHRGWAERSAELSAATSARLRRQRKAGRLRDDVPGDVLQCYLDLVLDGL
                     VARLASGEEPARLSAVLDLVEESVRNR"
     gene            complement(4234..7044)
                     /gene="acnA"
                     /locus_tag="VF874_02830"
     CDS             complement(4234..7044)
                     /gene="acnA"
                     /locus_tag="VF874_02830"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX9175055.1"
                     /translation="MSSGNSDNSSPNSFEARDTLKVGDKSYEIYRLDAVKGAEKLPYS
                     LKVLAENLLRNEDGLNITKEHIEAIANWDPEAEPSVEIQYTPARVIMQDFTGVPCIVD
                     LATMREAIDDLGGDADKVNPLAPADLVIDHSVIADLFGRADAFERNVEIEYERNGERY
                     QFLRWGQGAFSDFKVVPPGTGIVHQVNIEYLASVVMERDGVAYPDTCVGTDSHTTMVN
                     GLGVLGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIKPGVTATDVVLTVTQMLRE
                     HGVVGKFVEFYGEGVAEVPLANRATLGNMSPEFGSTAAIFPVDQVTIDYLKMTGRSDE
                     QCELVEAYTKEQGMWHEPDKEPKFSEYTELDLASVEPSIAGPKRPQDRIPLKTAKACF
                     RRDIKDYVGDDPKKEEYSKVDEAVDETFPASDPASLRYADDGEADVRSAANKSEGRDS
                     NPVKVKSAERGDFTLDHGAVVIAAITSCTNTSNPEVMLGAALLAKNAVEKGLESKPWV
                     KTSLAPGSQVVTDYYEKAGLTPYLEKLGFYLVGYGCTTCIGNSGPLPDEISQAVNDND
                     LAVTAVLSGNRNFEGRINPDVKMNYLASPPLVIAYALAGTMDFDFENDSLGLDEDGND
                     VYLKDIWPASEDINETIKSSINQEMFVKNYADVFKGDDRWRNLPTPEGNTFEWDEDST
                     YVRKPPYFDGMPEEPEPVSDIQDARVLALLGDSVTTDHISPAGSIKPGTPAAQYLDEH
                     GVDKNDYNSYGSRRGNHEVMIRGTFANIRLKNLLLDDVSGGYTRDFTQDDGPQEFIYD
                     AAQHYAEQDIPLVVLGGKEYGSGSSRDWAAKGTRLLGVRAVIAESFERIHRSNLIGMG
                     VIPLQFPEGKSAESLGLDGTEAYDITGIEELNDGKTPKMVHVKAGDVEFDAVVRIDTP
                     GEADYYRNGGILQYVLRSMLKS"
     gene            7209..7793
                     /locus_tag="VF874_02835"
     CDS             7209..7793
                     /locus_tag="VF874_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6676 family protein"
                     /protein_id="HEX9175056.1"
                     /translation="MAVTMPQIPLYIPAEVCATVGESPATPPDQCMSKVMVDVRDDGV
                     SAPKDADVAGLRQVVADAKKDGIDLKVVVMSQSPPIDTPLRDIATEVGKANPGSTVLV
                     ISPGWAGTYSTSYDRVTLEAGQDVAKTAGNPVQATKNFVSQLQTPDFPWTALTIVLVI
                     GVALATVLTRVLQVRSRRDAPADTTPASPAESTA"
     gene            7989..9398
                     /gene="ripA"
                     /locus_tag="VF874_02840"
     CDS             7989..9398
                     /gene="ripA"
                     /locus_tag="VF874_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family peptidoglycan endopeptidase
                     RipA"
                     /protein_id="HEX9175057.1"
                     /translation="MTRTRHSVCAAAVAVGLLLSTPGLAAAEPVPAGPNDIATLIAAV
                     ANANQQLQDIGAAVQTRQEAVNKAIVDVQTARDQADAAKADVAASQRAVEDANAAIAA
                     AQQRVDTYAASAYINGPSAFYLTATTPEDLIATASAGETLALSAKQTLADLQRARTEQ
                     VNRDSAARAAQQRADQAAADAQRSQEEAVAALTDAQREFGDQKAQVDRLAAERNAAQA
                     RLDAARATWSTQAATVPAPGAPAVAAPAPADRWDAPAANTPAPQANSRNWDASPWDPL
                     LPAIPSAFVSGDPIAIINAVLGISATSSQVTADLGRKFLTRLGILKPTDTGITNGQIS
                     RVYGRQASEYVIQRGMSQIGVPYSWGGGTAAGASIGQGDATVGFDCSGLVLYSFAGVG
                     IKLPHYSGAQYDMGRKIPAAQMRRGDVIFYGPGGSQHVTIYLGNGQMLEAQQSGVPVK
                     VSPVRTSGMTPYVVRYIEY"
BASE COUNT         1676 a         3230 c         2957 g         1587 t
ORIGIN      
        1 cgccgggcta cttatcagag gtccatcacg tcaacgaatg ggccgtgcac caccgcaccg
       61 acatcgacga cctgacattc gcctgcggac cctgtcacaa gctgctcgac gacgacggct
      121 ggaccacccg aaaaaacgcc gacggcgtca ccgaatggat tccgccgccc catctggatt
      181 tcggcaaacc cagaaccaac ggctactggc acccccaacg ctacttccga ctcgacgaca
      241 acgtcgacga cggcgaccct ccgtagccgc tgatgctcga tgtttgccaa atccgccggt
      301 ggctacccag ggacagagcc accagcgaag gagggcttgc gatgtccgca acgacacggc
      361 agacgcatca gaccgcagca gcagatcaat gggtgatccc tgtgatcgtc gcggcctgga
      421 tcgtcgttct gctcagcggc ttcttggtgc ttgtatagag ggaacatttc cgcagctgac
      481 gagcgttggg ctaagcatgg caacccaaga catcaccacc gatcagttca acgagaccat
      541 caacgacaac gaggtcgtgc tggtggattt ctgggcgtcc tggtgcggcc cgtgcaagca
      601 gttcgcgccg acgttcgagg cgtcttcgaa gaagcacccg aacgtcgtgt acgcgaaggt
      661 cgacaccgag gccgagcaag aactcgcggc gcgtgcacag attcgtgcca tccctacgct
      721 aatggccttc aagaagggca cgctggtgtt caaccaagcc ggcgccctga ccccgcccat
      781 gctcgaggat cttgttcagc agatcgaggc cctcgacgtc gaagccgccc tcgccgccca
      841 gaacgacggc gaacaggcat aaccggcggc acgcgatagc gtgcaacccg tgaacaacga
      901 cttcgtcctg gtcgaccgcc cacgcccgaa catcgcgctg atcacgctga accggcccga
      961 gcggatgaac tcaatggcgt tcgaagtgat ggttccgctc aggagcgtca tcgaagaggt
     1021 ctcctacgac aacgacgtcc gcgtcgtcgt cctcaccggc gcgggccgcg gattctcgtc
     1081 cggcgccgac cacaagtccg ccggatccgt cccgcacatc gacgggctca cccggcccac
     1141 cttcgcgctg cgctcgatgg aagtcctcga cgacgtgatc ctcggcctgc ggcgcatgca
     1201 tcaacccgtc gttgccgccg tcaacgggcc ggcgatcggc ggcggcctgt gcctggccct
     1261 tgccgccgac atccgcatcg cgtcgacgca ggcctacttc cgcgccgccg gcatcaagaa
     1321 cggcctgacc gccagcgaac tcgggctcag ctacctactc ccacgagcga tcgggtcatc
     1381 gcgagcgttc gagatcatgc tcaccggccg cgacgtcgac gccgatgaag ccgagcgcat
     1441 cggtttggtc tcccgcaagg tacccgcaga cgagctgctg gacacctgct atcagatcgg
     1501 cgagcagatc gccgcgttct cccggcccgg aattgagttg accaagcgca cgctgtggac
     1561 tggactcgac gccggtagcc tcgaagggca catgcaggcc gaaggtctgg gacagctctt
     1621 cgtgcgcctg ctcaccgcaa cttcgaagag gccgtcgccg cgcgtgccga gaaacgcaca
     1681 ccgaccttca ccgacgacaa gtaggggact tacgccgtga tcaccgcaac gaacctggag
     1741 gtccgcgccg gcgcgcggat tctgctgtcc gcggcgggtt ctgcgctgcg agtacagccc
     1801 ggcgatcgca tcgggttggt cggtcgcaac ggggccggca agaccaccac tatgcgcatc
     1861 cttgccggcg acgccgagcc gtacgcgggc accatccacc gtaagggcga aatcggttat
     1921 ctcccacagg atcccaaaga gggcaacctc gaagtcatcg cccgcgatcg cgtgctgtcc
     1981 gcccgcggtc tcgacaccct gatggccgac ctggaaaagc aacaggcgct gatggccgaa
     2041 gtcgtcgacg acatcgagcg cgataaagcc gtccgccgtt acggccagct cgaggagcgg
     2101 ttcgccgcgc tcggcggata cgtcgccgaa agcgaggccg cccgcatctg cgcgagcctc
     2161 ggcctgccgg accgcgttct gacgcagccg cttcgcacgc tgtccggcgg ccaacgccgc
     2221 cgcgtcgaac tggcccgcat cctgttcgcc gcatcggata ctggcgccgg ctcggacacg
     2281 acactcctcc tcgacgagcc caccaaccac cttgacgccg actcgctcgg ctggctaagg
     2341 gacttcctgc agaaccacgc cggcggcctc gtggtgatca gccacaacgt cgacctgctt
     2401 gccgacgtcg tcaacaaggt gtggttcctc gacgcggtgc gcagcgaaat tgacgtctac
     2461 aacatgggct ggcacaagta cctcgacgcg cgggccaccg acgaacaacg ccgccgccgt
     2521 gaacgggcca acgccgaacg caaagcggcg gcactcagga cccaggcggc gaagctcggc
     2581 gctaaagcca ccaaagccgt tgcagcgcag aatatgctga gacgcgcgga ccgcatgatg
     2641 gccgcgctgg acgacgagcg ggtggccgac aaggtcgccc ggatcaaatt cccgacacca
     2701 gcgccgtgcg ggcgaacccc attgacagcc aagggcctga cgaagaacta cggctccctg
     2761 gaagtgttca gcggcgtcga tctcgcgatc gaccgcgggt ccagggtggt ggtgctcggc
     2821 ctcaacggcg cgggcaagac cacgctgtta agactcctgg ccggcgtcga gacccccgac
     2881 accggtgtgc tcgagcccgg acacggctgc aggatcggct atttcgcaca ggaacacgac
     2941 acgctggaca acatggcgac cgtgtgggaa aacatccggc acgcggcacc ggacagcggc
     3001 gaacaggatc tgcgcgggct gctcggcgca ttcatgttca gcgggccgca actagaccag
     3061 cgcgccggca cgctgtcggg cggcgagaag acgcgcctcg cgctcgccgg cctggtggcg
     3121 tcgaccgcga acgtgctttt gctcgacgag ccgaccaaca acctcgaccc cgcatcacgc
     3181 gaacaggtgc tcgacgcgct gcgcagctac tgcggcgctg tcgtgctggt cacccacgac
     3241 cccggcgccg ccgaggccct cgccccgcaa cgcgtggtgc tgcttcccga cggcaccgaa
     3301 gactactggt ccgaggacta ccgcgacctg atcgagctgg cctgaaacgg caactcattt
     3361 caccaattcc gcccgtcgcc gagccgcgtt ccctaccctc gaggaacggg tgctcacaca
     3421 gcggggagaa ggcgatgagg aatccggaca agggtcgcga ggacttgcta caccagcttc
     3481 gggatcaata cgagcacggc gcgagcatca ggaagctggt ggcgtcgacc ggtcgttcct
     3541 acggttcgat tcacagcatg ttgcgcgaat cgggcacgac gatgcgcggc cgcggcggcc
     3601 cgaaccacac ccgcagacga gccagttgac gcgttttctt ctgctcctcg cgtccgctag
     3661 cgattccgca ctgactcctc gaccagatcg aggaccgctg acagccgcgc cggctcctcc
     3721 ccggacgcga gccgtgccac caacccgtcg agcaccaggt cgaggtaaca ctgcagaacg
     3781 tcgccgggaa cgtcgtcacg caaccgcccc gctttgcgct gccgtcgtag ccgcgcagat
     3841 gtcgcggccg acagctccgc ggatcgctcc gcccagccgc ggtggaacgc cggatcgtta
     3901 cgcagtttgc gtgcgatctc caaacgtgtt gcgagccagt cgaattgatc tggattggcg
     3961 agcatctccc gcatcacctg aatcagtccc tcgcgcgatg cgacgtcggc catccgctcg
     4021 gcgtcttcgc gagccagctc gaaaaacagt gcgtccttgt cccggaagtg atggaagatc
     4081 gcaccgcgcg acagcccgat cgactgctct agacgacgta ccgtggccct gtcatagccg
     4141 tgctgtgcaa agcaccggcg ggcgccgtcg aggatctgac ggcgacgagc cgccagatgt
     4201 tcctcgctga ctcggggcat cgtccgcggc cgctcaggac ttcagcatgc tgcgcaacac
     4261 gtactgcagg atgccgccgt tgcggtagta gtcggcttca ccgggagtgt cgatgcgcac
     4321 caccgcgtcg aactcgacgt caccggcttt gacgtgcacc atcttcggtg tcttgccgtc
     4381 gttgagttcc tcgattccgg tgatgtcgta ggcctcggtg ccatccagcc ccagcgactc
     4441 ggctgactta ccctcaggga actgcagcgg gatcacgccc atgccgatca ggttcgagcg
     4501 atggatgcgc tcgaacgact cggcaatcac cgcgcgcacc ccgagcagcc gggtgccctt
     4561 ggcggcccag tcgcgcgacg acccggagcc gtactccttg ccgcccagca cgacgagcgg
     4621 aatgtcttgt tccgcatagt gttgcgccgc gtcgtagatg aactcctgcg gcccgtcatc
     4681 ttgggtgaag tcgcgggtgt agccacctga cacgtcgtcg agcagaaggt tcttcaaccg
     4741 gatgttggcg aacgtgccgc ggatcatgac ctcgtggttg ccgcgccgcg agccgtagga
     4801 gttgtagtca ttcttgtcca ctccgtgctc gtccaggtac tgcgcggccg gggtgcccgg
     4861 cttgatgctg cccgccggcg aaatgtggtc ggtggtcacc gaatcgccga gcagcgccag
     4921 cacccgagcg tcctggatgt cgctgaccgg ctcgggctct tcgggcatcc cgtcgaagta
     4981 cggcggcttg cgcacatacg tcgaatcctc gtcccactcg aacgtgttgc cctccggcgt
     5041 cggcaggttg cgccagcggt cgtcgccctt gaacacgtcc gcgtagttct tgacgaacat
     5101 ctcctggttg atcgacgact tgatcgtctc gttgatgtcc tcgctcgccg gccagatgtc
     5161 cttcaggtag acgtcgttac cgtcctcgtc gagaccaagc gagtcgttct cgaagtcgaa
     5221 atccatcgtt ccggcaagcg cgtacgcgat cacgagcggg ggagacgcga ggtagttcat
     5281 cttgacgtcc gggttgatcc ggccctcgaa gttgcggtta ccggacaaca ccgcggtgac
     5341 cgccaggtcg ttgtcgttga cggcctgcga gatctcgtcc ggcagtggtc ccgagttgcc
     5401 gatacacgtc gtgcaaccgt agccgaccag gtagaaaccc agcttctcca gatacggtgt
     5461 cagcccggcc ttctcgtagt agtcggtgac gacctgcgat cccggcgcga gcgatgtctt
     5521 cacccacggc ttcgactcga gccccttctc gaccgcgttc ttggccaaca gggccgcgcc
     5581 cagcatcacc tcggggttgg aggtattggt gcacgacgtg atcgccgcga tcaccaccgc
     5641 gccgtggtcc aacgtgaagt cgccgcgttc cgctgacttc accttcaccg ggttggagtc
     5701 ccggccctca gacttgttgg ccgccgaccg cacgtcggcc tcgccgtcgt cggcgtagcg
     5761 gagggatgcg ggatcactgg ccgggaacgt ctcgtcgacc gcctcgtcga ctttggagta
     5821 ctcctctttc ttcgggtcgt cgccgacgta gtccttgatg tccctgcgga agcacgcctt
     5881 cgcggtcttc aacgggatgc ggtcctgcgg acgcttcggg ccggcgatcg acggttcgac
     5941 gctggccagg tccaactcgg tgtactcgga gaacttcggc tccttgtccg gctcgtgcca
     6001 catgccttgt tccttggtgt aggcctcgac gagctcgcac tgctcgtcgc tgcggccggt
     6061 catcttcagg tagtcgatgg tgacttggtc gaccgggaaa atcgctgcgg tggaaccgaa
     6121 ttcggggctc atatttccca gcgttgcgcg gttggccagc ggcacctcgg ccacgccctc
     6181 gccgtagaac tcgacgaact tgcccaccac gccgtgctcg cgcagcatct gagtgaccgt
     6241 caggaccacg tcggtggccg tgacgccggg cttgatttcg ccggtcagct tgaagccgac
     6301 gacgcggggg atgagcatcg acaccggctg accgagcatc gcggcctcgg cctcgatgcc
     6361 gccgacgccc cagcccagca cgccgagccc gttgaccatc gtggtgtgcg agtcggtgcc
     6421 gacacaggtg tcgggatagg ccactccgtc gcgctccatc acgacgctcg ccaggtactc
     6481 gatgttcacc tggtggacga tgccggtgcc cggaggcacc accttgaagt cgctgaaggc
     6541 gccctggccc cagcgcaaga actggtagcg ctcgccgttg cgctcgtact cgatctcgac
     6601 gttgcgctcg aacgcgtcgg cgcggccgaa caggtccgcg atcaccgagt ggtcgatcac
     6661 caggtcggcc ggtgccagcg ggttcacctt gtcggcatcg ccgcccagat cgtcgatcgc
     6721 ctcgcgcatg gtcgccaggt cgacgatgca cggcacgccg gtgaagtcct gcatgatcac
     6781 ccgcgccggc gtgtactgga tttcgacgct cggctccgcc tcgggatccc agttcgcgat
     6841 cgcctcgatg tgctctttcg tgatgttcaa gccgtcctcg ttgcgaagca ggttctcggc
     6901 cagcaccttc aggctgtagg ggagtttctc ggcgcctttg acggcgtcga ggcgatagat
     6961 ttcatagctc ttgtcgccga ccttcagggt gtcgcgggct tcgaaagagt taggggacga
     7021 attgtccgaa ttaccgctgc tcacattcac tcccagttaa gttgtcggcc ggcggcgggc
     7081 tgtgtcgccg gcacttccga tcctaacagt acgcttgtcc tttttcacca ggcggtgtcg
     7141 gtcttcaagg gggtgtaccc acttcgtcgt ctgctcttcg cgcgagcgct catcgccgcg
     7201 gtacggtcat ggccgtgacg atgccgcaga ttcctctcta tatccctgcg gaggtgtgcg
     7261 cgaccgtcgg cgagtccccg gcgacgccac cggaccagtg catgagcaaa gtgatggtcg
     7321 acgtccgcga cgacggagtg agcgcgccca aggacgccga cgtcgccggc cttcgacagg
     7381 ttgttgccga cgccaaaaag gacggcatcg acctcaaggt cgtcgtgatg tcgcagagcc
     7441 cgccgatcga caccccgctt cgcgacatcg ccaccgaggt cggcaaggcc aacccgggtt
     7501 ccacggtcct tgtcatcagt cccggctggg ccggcaccta cagcacgagc tacgaccggg
     7561 tgaccctgga agccggccag gacgtcgcca agaccgccgg taacccggtc caagcgacga
     7621 agaacttcgt cagtcagctc cagaccccgg atttcccgtg gacagcgctg acgatcgttc
     7681 tcgtgatcgg agtcgcactc gcgacggtgc tcacccgggt gctccaggtg cgcagcagac
     7741 gtgacgcgcc cgccgacacc acacctgcgt cacccgctga atcgacggcc tgacgccgcg
     7801 cttatttcgc atttcaagcg ggcggaattg tcgcccagat aactaattgg tcacagcaac
     7861 ctcaattaca ttcttgtagt ttgtgaccaa agtttcgtca gtgtcaaaag tgacgtacgg
     7921 tgcccttgac tacttatgtt gtagaagtgt ttccagtgat ctggacgccg atagggagac
     7981 aaagtcgtat gacacgcacc cgtcactcgg tatgcgcggc cgcagtggcc gtcgggctgt
     8041 tgttgtcgac cccgggactg gcggccgctg aacccgtccc ggccgggccg aacgacatcg
     8101 cgactctgat cgcagccgtc gccaacgcga accaacagct ccaggacatc ggcgccgcgg
     8161 tgcaaacccg tcaggaggcg gtgaacaagg cgatcgtcga cgtgcagacc gcgcgggatc
     8221 aggccgacgc ggccaaggcg gacgtggcgg ccagccagcg cgcagttgaa gacgccaatg
     8281 cggcgatcgc ggcggcgcag cagcgcgtcg acacatacgc ggcctctgcg tacatcaatg
     8341 gaccgtcggc gttctacctg acggcgacga ctcctgagga cctgatcgca acggcctcgg
     8401 ccggcgagac actggcgctc agcgccaagc agacgctggc cgacctgcag cgcgcgcgca
     8461 ccgagcaggt caacagggac tcggcggcac gggcggcgca gcagagagcc gatcaggcgg
     8521 cggccgacgc ccagcgcagc caggaagagg ccgtcgcggc cctgaccgac gcgcagcgcg
     8581 agttcggcga ccagaaggcg caggtcgacc ggctggccgc cgagcgcaat gccgcgcaag
     8641 cgcgcctgga cgccgcccgc gcgacgtggt cgacgcaggc cgccaccgtg ccggcacccg
     8701 gggcccccgc agtcgcggct cccgcaccgg cggatcgctg ggacgcccct gccgcgaata
     8761 cgcccgcgcc gcaagccaac tcgcgcaact gggacgccag tccatgggat ccgctgctgc
     8821 ccgcgatccc cagcgcgttc gtcagcggcg acccgatcgc gatcatcaac gcggtgctcg
     8881 ggatctcggc gacctcgtcg caggtcaccg cggatctcgg ccgcaagttc ctgacgcggc
     8941 tgggcatcct gaagcccacc gacacgggca tcaccaacgg gcagatctcg cgggtgtacg
     9001 ggcggcaggc ctccgagtac gtgatccagc ggggcatgtc gcagatcggc gtgccgtact
     9061 cgtggggcgg cggaaccgcc gccggggcca gcatcggcca aggcgacgcc actgtgggct
     9121 tcgactgctc aggactggtt ctgtactcgt tcgccggtgt cgggatcaag ctgccacact
     9181 actccggcgc tcaatacgac atgggccgca agatcccggc agcccagatg cgccgcggcg
     9241 acgtcatctt ctacggcccc ggcggcagcc agcacgtgac catctacctc ggcaacggcc
     9301 agatgctgga ggcccaacaa tccggagtgc cggtgaaagt ctcgccggtg cgcaccagcg
     9361 gcatgacgcc gtacgtcgtg cgctacatcg aatactgacc aggaggggaa gcgcttttgc
     9421 gtccgactcg tctgctgcgg atcgtcggtg
//
LOCUS       DASWBF010000089         4399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_403932,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000089 DASWBF010000000
VERSION     DASWBF010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403932"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VF874_02845"
     CDS             complement(<1..342)
                     /locus_tag="VF874_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175058.1"
                     /translation="MLHEIALLRLRALLARAHGDEASSRDHRDRYRAMAISLGFEGQI
                     ERAEAMTRPRPSGVRGVRPLKVMVANQGLAPMGWLKPSRDKNGLDESRVLQPRPAVVW
                     ERSSGFEPPNRV"
     gene            complement(553..2241)
                     /locus_tag="VF874_02850"
     CDS             complement(553..2241)
                     /locus_tag="VF874_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9175059.1"
                     /translation="MFEVAIDQRTSIPLAESPDIYGAYPRLSDDQIARLEAGGARRAV
                     KAGEKLVREGERSDYFFVILSGKVAVTTTDDAGNRHVIRVHGPGRFLGELGDLEGQAA
                     FYTAEAVEPGEVLVVPTERVRALVAHDPVLSDLILRAYLLRRSLLIQEGSGFRIIGSC
                     YSPDTARLREFAARNRLPHRWLDLERDKHAERLLQRFGVSPQDTPVVIWGGEVLRNPT
                     NTELARRVGLPVPDTVPDESDVVVVGAGPAGLGAAVYAASDGLTTAAMERIATGGQAG
                     TSSKIENYLGFPGGISGADLAERAVLQAGKFGARIIVSAEITRLESGGGQHRVTLADG
                     GSVVARAVVLATGARYRRLAVPGIESFEGNGVYYAATFQEALMCGTGPVVIVGGGNSA
                     GQAAIFLAARVSRVYLVIRGDDLNKTMSRYLVDPINRHPRVTVRSCTEIREVHGNGAL
                     TAVVTEDKRTGERQSIQTRALFVFIGADPNTSWLAGAIELDDRGFVPTGPAALYSNAD
                     GARRSTQRQPMMLETSQPGVFAAGDVRSGSVKRVASAVGEGSMAIRQIHEYLGT"
     gene            complement(2234..2497)
                     /locus_tag="VF874_02855"
     CDS             complement(2234..2497)
                     /locus_tag="VF874_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005561811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX9175060.1"
                     /translation="MTSMDIDPHVAAIRQVRPRTNGCEECLRLGTPWVHLRLCLTCGH
                     VGCCDSSPMRHARAHAGTIGHPVVQSMEPGENWRWCYVHENYV"
     gene            complement(2494..2946)
                     /gene="trxA"
                     /locus_tag="VF874_02860"
     CDS             complement(2494..2946)
                     /gene="trxA"
                     /locus_tag="VF874_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005259202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9175061.1"
                     /translation="METDIVKCPHCGKRNRVPAAAAGKPRCANGHQWLPWIAAAGDGD
                     FAAVAEKSSVPVLVDLWATWCGPCRMVSPALEQLATERAGQLKLVKVDVDKAPAISQR
                     FTVQAVPTLLLLDHGQILARQAGAAPVAALRNWLDQALSAGSAKEARS"
     gene            complement(2960..>4399)
                     /locus_tag="VF874_02865"
     CDS             complement(2960..>4399)
                     /locus_tag="VF874_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9175062.1"
                     /translation="IDLVDEACARLRTEIDSLPAELDEITRHVTRLEIEEVSLAKESD
                     PASKARLEELRRELADLRAEADAMHAQWDAERQAIKRVQQLREQLEQVRQEAEEAERN
                     YDLNRAAELRYGQMRELERKLQAAEEQLRGKQGERPLLREVVTEDEIAEIVSVWTGIP
                     VSRLQEGEREKLLRLDEILHERVIGQEEAVQLVADAVIRARAGIRDPRRPIGSFIFLG
                     PTGVGKTELAKTLAAALFDSEDNMVRLDMSEYQERHTVSRLVGAPPGYVGYDEGGQLT
                     EAVRRKPYSVVLFDEIEKAHTDVFNTLLQVLDNGRLTDAQGRQVDFRNTVVIMTSNIG
                     SHYLLDGVTADGEIKPDARDRVMAELRGHFRPEFLNRVDDIVLFTPLSMPQIERIVEL
                     QLAELRDRLAERQIDLDISVEARRKIAEHGYDPVYGARPLRRYIAHEVETKIGRALLR
                     GDITGGGKIRITVETGELAVGYSEPALAA"
BASE COUNT          678 a         1534 c         1401 g          786 t
ORIGIN      
        1 cacgcgattt ggcggttcga atccggaaga ccgttcccag acgacggcgg ggcgaggttg
       61 tagtacccgg ctctcatcca acccgttttt gtcacgtgac ggttttagcc aacccatagg
      121 ggccaggccc tgattggcca ccatcacttt taagggccgc acgccgcgca cgccactcgg
      181 ccgcggtctt gtcatcgcct cggcccgctc gatctgccct tcaaatccaa gcgatatcgc
      241 catggctcgg tagcggtccc gatggtctcg agaggaggct tcatcgccgt gggctcgagc
      301 cagcagcgcg cgcagccgta gcagtgctat ctcgtgcagc acgaatccgg ggtgggtcgg
      361 gacggcagcc agccggtcga tcgcggcctg cgcatcctcg aaagatctcc gtcgccggcg
      421 cgttgccact gtgctcccac caatacggag gtggccaggg cactccaaga cagaccggtc
      481 tgagtcaggc tgaccggttc agtattgccg aatgccgggc aggtgacggg tcggggcggt
      541 ccgcgctgcc gactacgtcc cgaggtactc gtggatttgg cggatcgcca tcgatccctc
      601 gccgaccgcg gaggccaccc gtttgaccga gccgctacgc acgtctcccg ccgcgaacac
      661 accgggctgg ctggtttcca gcatcatcgg ctggcgctgg gtgctgcgcc gggcaccgtc
      721 ggcgttcgaa tacagcgctg ccggaccggt cggcacgaag ccgcggtcgt cgagttcgat
      781 ggcgccggcg agccatgagg tgttgggatc cgcgccgatg aagacgaaca gggcgcgggt
      841 ctggatcgac tgccgctcgc ctgttcgttt gtcctcggtc accaccgccg tgagtgcacc
      901 gttgccgtgc acctcccgga tctcggtgca cgaccggacg gtcacgcggg ggtgccggtt
      961 gatcgggtcg acgagatacc gggacatggt tttgttcagg tcgtcgccgc ggatcaccag
     1021 gtacacccgg gaaacccgcg cggcgaggaa gatcgccgcc tggcccgcgg agttaccacc
     1081 cccgacgatg acgaccggcc cggtgccgca catcaacgcc tcttgaaagg tggcggcgta
     1141 gtacacgccg ttgccctcga acgattcgat tcccgggacg gcgagccttc gatacctggc
     1201 tcccgtggcc agcaccacgg ccctggccac caccgaccca ccgtccgcga gcgttacccg
     1261 gtgctggccg ccgccggatt ccagccgggt gatctccgcg gagacgatga tccgcgctcc
     1321 gaacttgccg gcctgcagaa ctgcccgttc ggcaaggtcg gctccggaga ttccgccggg
     1381 gaatccgaga tagttctcga tcttcgacga cgtcccggcc tgcccgccgg ttgcgatccg
     1441 ctccatggcc gccgtggtca ggccgtccga cgcggcatac accgcagcgc ccagccccgc
     1501 cggaccggcg cccaccacga cgacgtcgga ttcgtcgggc accgtgtcgg gtaccggcag
     1561 tcccacccgg cgggccagct cggtgttggt cgggttgcgc agcacctcac cgccccagat
     1621 caccaccggg gtgtcctggg gcgatactcc gaaccgctgc agcagccgtt cggcgtgctt
     1681 gtcacgttcc aggtcaagcc agcggtgcgg caatcggttt cgcgcggcga actcgcgcaa
     1741 ccgggcggtg tccggcgaat agcaggagcc gatgatccgg aatccggatc cctcctgaat
     1801 gagcagcgag cggcgcaaca gatacgcgcg caggatgagg tcactgagca ccggatcgtg
     1861 ggcgaccagg gcccgcaccc gctctgtcgg gaccaccagc acttcgccgg gttccaccgc
     1921 ttcggctgtg tagaacgccg cttggccttc gaggtcgccc agctccccga gaaaccgccc
     1981 ggggccgtgc acccggatca cgtgccgatt gcccgcgtcg tccgtggtgg tgacggcgac
     2041 tttgccggac agaatgacga aaaagtagtc ggagcgctca ccctcgcgga ccagcttctc
     2101 tcccgctttg actgctcgcc gggcgccgcc cgcctcgagc ctggcaatct gatcgtcaga
     2161 cagtcgcggg tacgcgccgt atatgtcggg ggattcggcc agcggaatcg atgtgcgctg
     2221 gtcgatggcg acctcaaaca tagttctcat ggacgtagca ccagcgccag ttctcgccgg
     2281 gctccatgga ctgcacgacc gggtgcccga tggtgccggc gtgcgcccgg gcatgccgca
     2341 tcggtgacga gtcgcagcag ccgacatgcc cgcacgtcag gcacagccgt aggtgcaccc
     2401 acggtgtgcc gagccgcaga cattcctcgc agccgttcgt gcgcgggcgc acctggcgga
     2461 tcgccgccac gtgtggatcg atatccatgg atgtcatgac cgtgcctcct tcgcgctgcc
     2521 ggccgatagc gcctggtcca gccaattgcg cagcgccgca acgggagcgg cacccgcttg
     2581 ccgcgccagt atttgcccgt ggtcaaggag cagcagtgtc ggcaccgcct gcaccgtgaa
     2641 ccgctgcgaa atcgccggtg ccttgtccac gtcgaccttg acaagtttga gttgtccggc
     2701 gcgttcggtg gcgagctgct ccagcgccgg gctcaccatc cggcacggtc cgcaccaggt
     2761 cgcccatagg tcgacaagca ctggaaccga ggacttttcg gcaacggcgg cgaagtcccc
     2821 gtcgccggcc gcggcgatcc acggcagcca ctggtgaccg ttggcgcacc ggggctttcc
     2881 cgcggccgcc gcgggtaccc ggttgcgctt tccgcagtgc gggcatttca cgatgtcggt
     2941 ttccatggcg cttcctccgt caggccgcca gggccggttc cgaatagccc acggcgagtt
     3001 cgccagtctc gacggtgatg cggatctttc caccgcccgt gatgtcgccg cgcagcaacg
     3061 cccggccgat cttggtttcc acctcgtggg cgatgtagcg gcgtagcggc cgcgccccgt
     3121 acaccgggtc gtagccgtgt tcggcgatct tccggcgcgc ctcgacactg atatcgagat
     3181 caatctgcct ttccgccaac cgatcccgca gctcggccag ctgcagctcg acgatccgct
     3241 cgatctgcgg catcgacagc ggagtgaaca acacgatgtc gtcgacccgg ttgagaaact
     3301 cgggccggaa gtgcccgcgc aactcggcca tcacccggtc gcgcgcgtcc ggcttgatct
     3361 cgccgtccgc ggtgacgccg tcgagcagat agtgcgagcc gatgttggag gtcatgatga
     3421 ccacggtgtt gcgaaaatcg acctgacgcc cctgcgcatc ggtgagccgg ccgttgtcga
     3481 gcacctgcag cagggtgttg aacacatcag tgtgcgcctt ttcgatctcg tcgaacagca
     3541 ccaccgagta gggcttgcgg cgcaccgctt cggtgagctg accaccctcg tcgtagccga
     3601 cgtatcccgg tggcgcaccg accagtcggc tcaccgtgtg ccgctcttgg tactcgctca
     3661 tgtccaaacg gaccatgttg tcctcgctgt cgaacagcgc ggcggccaac gttttcgcga
     3721 gttcggtctt accgaccccg gtgggtccga ggaagatgaa cgagccgatc gggcggcgag
     3781 gatcccggat tccggcgcgg gcccggatca ccgcatcggc gaccagctgg accgcttctt
     3841 cctggccgat cacccgctca tgcaggatct cgtcgagtcg cagcagcttc tcgcgctcac
     3901 cctcctgcag ccgcgacacc gggatgcccg tccataccga cacgatctcg gcgatctcgt
     3961 cctcggtgac gacctcgcgg agcaacggcc tctcgccctg cttgcctctc aactgctcct
     4021 cggcggcctg cagtttgcgt tccagttcac gcatctggcc gtagcgcagt tccgcggccc
     4081 ggttgagatc ataattgcgc tcggcttcct cggcctcctg ccgcacctgc tcgagttgct
     4141 cgcgaagttg ctgcacccgc ttgatcgcct gccgttcggc gtcccattgc gcgtgcatgg
     4201 cgtcggcctc agcgcgcagg tcggccagct ccctgcgcaa ctcttccagg cgcgccttgc
     4261 tcgcgggatc ggattccttg gccagcgata cttcttcgat ctccagtcgc gtcacgtgac
     4321 gggtgatctc gtccaattcg gctggcaacg agtcgatttc ggtgcgcagc cgcgcgcaag
     4381 cctcgtccac caggtcgat
//
LOCUS       DASWBF010000090         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_991473,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000090 DASWBF010000000
VERSION     DASWBF010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991473"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..779
                     /locus_tag="VF874_02870"
     CDS             195..779
                     /locus_tag="VF874_02870"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX9175063.1"
                     /translation="MPSLGEAEWLNSEPPGPAGLRGRVVLVNFWTLTCINWLRQQPYV
                     RAWSQAHRDDGLIVTGVHTPEFSFEHEIDRVRQAVKERGIDYPVAADNDYAIWTAFGN
                     HCWPALHVVDTDGMIRDEHYGEGRYEQSERTIQRLVDRAPAFYSGVENDLHKCGTTTS
                     SDRKAGVDQCPCASVPCRGCVPATRPAELRRRQG"
     gene            805..1476
                     /locus_tag="VF874_02875"
     CDS             805..1476
                     /locus_tag="VF874_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX9175064.1"
                     /translation="MATNSMATDGTRVPRALTHDEAATLFGRTMTLVAGTTGLFALGA
                     YVGRNVPPQLGWLWFVAAFVTLLGIDRAIRRSERLALTLLFAFGALLGFAVAPTIAYY
                     AAADPQALWQAGGATALFVAGFGAAGYATSHDLSALARYLFLALIALVLFGVVAIFVQ
                     IPGASLVYAIVGLAIFAGLTAFDFQRLRRSKDIRTAPLLAASIFLDILNVFLLLLSIF
                     GGRDE"
     gene            1774..3135
                     /locus_tag="VF874_02880"
                     /pseudo
     CDS             1774..3135
                     /locus_tag="VF874_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
     gene            3230..3544
                     /locus_tag="VF874_02885"
     CDS             3230..3544
                     /locus_tag="VF874_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175065.1"
                     /translation="MVVAFGLSAELGPVSYTTGTQEYLGPGQPQRPYSEETQRSVDQE
                     VARLLREAEQTSLELLRTHRDALDRLTEMLLTEETVDGSVVLATLGDNALLATLQPTG
                     GN"
     gene            complement(3767..>4203)
                     /locus_tag="VF874_02890"
     CDS             complement(3767..>4203)
                     /locus_tag="VF874_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175066.1"
                     /translation="ALSDDARRLLLVAAAEPVGDPLPLLRASERLGIAVAVVGAETDG
                     LFAIGDRVTFRHPLVRSAVYRSVAVEGRRAVQQALAEATDSVTDPDRRAWHLAAAAHS
                     NAGSDSRCAAASTPPMMTAVSPGRTSPANSAASANIRMPMTT"
BASE COUNT          709 a         1529 c         1316 g          649 t
ORIGIN      
        1 ccatcagcgt ctttcccgac gaggtctatc aggctccgcg gagctgggcg gagcaggcgt
       61 atcccaacct catccactat aaccaggtcg acaagggcgg gcacttcgcc gcctgggaac
      121 agcccgagct gttctgcgac gaggttcgcg tcggcttccg gtcgctccgg taggtcaccg
      181 tgtctcgtgt gcatatgccc tcgctcggcg aggccgagtg gctcaactcc gagccacccg
      241 gccccgccgg gcttcgcggc cgcgtggtcc tggtgaactt ctggacgctg acctgcatca
      301 actggctgcg ccagcaaccc tacgtccgcg cctggtcgca ggcccaccgg gacgacgggc
      361 tgatcgtgac cggcgtccac accccggagt tctccttcga gcacgaaatc gaccgggtcc
      421 ggcaggcggt gaaggagcga ggaatcgact accccgtcgc ggccgacaac gactacgcga
      481 tctggaccgc gttcggcaac cactgctggc cggcgctgca cgtcgtcgac acggacggga
      541 tgatccgcga cgagcactac ggcgaaggac gctacgagca gtcggagcga actatccagc
      601 ggctcgttga tcgggcgccc gccttctaca gcggtgtgga gaacgaccta cacaaatgcg
      661 gtaccaccac cagctctgac cggaaggcag gcgtggatca atgtccttgc gcgtctgtcc
      721 cgtgccgcgg atgcgtcccc gccacacgac cggcggaact acgacggcga caaggctgac
      781 cacgaggaaa cctggagcaa gaaaatggcg acgaacagca tggccaccga cggcacccga
      841 gtgccgcgtg cactgaccca cgacgaagca gcaaccctgt tcgggcgcac catgacgctg
      901 gtcgcaggca ccaccgggct gttcgctctc ggcgcctacg tcggccgtaa cgtgccccca
      961 cagctcggct ggctgtggtt cgtggctgcc ttcgtcacgc tgctcggcat cgatcgcgcg
     1021 atacgcagat ccgaacgcct cgccctgacc ctgcttttcg cgttcggggc tctcctggga
     1081 ttcgcggtcg cccccacgat cgcctactac gcggcggccg acccgcaggc tttgtggcaa
     1141 gccggcgggg caacggcgtt gttcgtagcc gggttcggag ccgccggcta cgcaactagc
     1201 cacgatctgt cggcgctcgc ccgctacctc tttctcgcgc tgatcgcgct ggttctcttc
     1261 ggcgtcgtcg cgatcttcgt ccagattccc ggggcatccc tcgtctacgc gatcgtcggc
     1321 cttgccatct tcgccggcct cacagccttc gacttccagc ggctgcgccg cagcaaggac
     1381 atccgcaccg caccgctgct cgcggcgtcg atcttcctcg acatactcaa cgtcttcctg
     1441 ctccttctgt cgatcttcgg cggccgcgat gagtgacgga cgtgcccggc cgctgactcg
     1501 cacaacgccg ccgggtgcct gggtagcccc agtcagccgc ttcgcccacc aactagaacc
     1561 gggagccact atcgtgaccc gcaatccgat gatgaagtcc accgcgacga tcctcggcga
     1621 cgctgacgga gtagcgccgt gaccgcccca gcgaaatccg ggccaactcg ggacacaccg
     1681 ccgaagccgg caccgccgcc tccgcccgcg tggcggccgt ggctgctgcc gatcggcctg
     1741 ctgatcacga caagtctgct gttcatcccg gtcatgacca gccccgcgcc gccacagcga
     1801 ctcgcctaca gcgacctgct caaccaggtc aatgcgggcc acgtcacctc ggtcagcatc
     1861 aacaaccttg gcgcggtgac cggccggctc gacaaggacg gggagttcac cagccagatc
     1921 cctaccgcgc tcacgaccgg gaccagcgac ctgcagcaga cgctcctcgc gcaccacgtc
     1981 acgatcaccg gcaagcagag cagtaactct gccctgctca acatcgcgct cagcttcctg
     2041 ccgctgctgc tgatcatcgg gctgtttgtc tggagcggcc gggccgcacg ccggtccctg
     2101 ggcgccggga tcggcggtat cggccgctcc cgcgccaaga tcaccgacgc ggaacggccc
     2161 aggacacggt tcagcgacgt cgcgggatac gacggcgcaa aggaggagat caccgaggtc
     2221 gtcgatttcc tgcgcactcc cgaccggtat gccaaggcgg gcgctgtggg gccgcgcggc
     2281 gtcttgatgg tcgggcctcc gggcaccggc aaaaccctgc tggcgcgcgc cgtcgccggt
     2341 gaggcagacg tgccgttcct gtcgatcacc ggatccggat tcgtcgagct gttcgtcggg
     2401 gtcggcgcgt cacgggtgcg tgatctgttc gccgacgccc gcaaacggtc ccccgccatc
     2461 atcttcatag acgagatcga cgccatcggc ggacgccggg gcggctcggc cttcgccacg
     2521 aacgacgaac gcgagcagac actcaatcag ctgctggccg agatggacgg cttcgacaag
     2581 gaagcagacg tcgtggtcct cgccgccacc aaccgccccg agaccctcga cccggcattg
     2641 gtccgaccgg ggcggttcga ccggcaggtc gtcgtaccgc tgccgaacca agtggaacgg
     2701 gcagagatcc tgcgcgttca tgcctgcggc aagaacatca gccgcgacgt cgacctcaat
     2761 gtcagtgccc gtgccacacc gggattttcc ggtgccgacc tggcgaacct gctcaacgag
     2821 gcagcgatca atgccgtgcg cgcggaccgg gacgtgatcg acgcagccga cctcgacgcg
     2881 gcccgcgacc gcatcctgct cggcaggcgc gatgcgtcca acgcgctcct tcccgaggag
     2941 cggcgatcgg tggcggtcca cgaggccggt cacgccctgg tcgcagccct gtcgccgcac
     3001 gccgaccccg tggccaaggt gaccatcctt cccgcaggta tggctctcgg cgtcaccgag
     3061 cagctgcccg aggccgagcg gcacctgtac accgagggct acctgaccga cacgctcgcc
     3121 gtgcgcctgg gtggtcgcgc ggccgagctg atcgccctgg gggaaggatc caccggcgcc
     3181 tcgaacgacc tggctgacga cggccggtgc gacacggctg gcgacccgca tggtggtcgc
     3241 gttcggactg tccgcggaac tcggcccagt cagctacaca accggaaccc aggagtacct
     3301 cggccctggc cagccccagc gaccgtactc agaagaaacc caacgatcgg tggaccaaga
     3361 agtcgcccgt ctactccgcg aagcggaaca gacctccctc gaactgttgc ggacacatcg
     3421 cgacgccctc gaccgtctca ccgagatgct cctgacagaa gaaacggtcg acggcagcgt
     3481 cgtcctcgcg accctaggcg acaacgcact cctggcaaca ctgcaaccga ccggcggtaa
     3541 ctgagatgaa cgacgctgaa aggttgccga cctcatcacg cgggtcactc gacagtgccc
     3601 aagctgatga acagcacgtc ggttcccggt tggacggacg gcaagtgacc agctgaatcg
     3661 cgggaacccg tgacggaggt ccgctcccgc cgccatcgcc ggggcacgga cggtggccgg
     3721 tgagccttct gatcgaccac ctgctcggtg tcgctcccgc ggtcgcctac gtcgtcatcg
     3781 gcatcctgat gttcgccgag gcagcgctgt tcgccgggct cgttctgccg ggtgagaccg
     3841 ccgtcatcat cggcggggtg ctggcggctg cgcatcggct gtcgctgccc gcgttactgt
     3901 gcgcggccgc ggccagatgc catgcacgac ggtccgggtc tgtcacgctg tcggtcgcct
     3961 cggccaacgc ctgctggacc gcgcggcgtc cctccacagc caccgaccga tacaccgccg
     4021 accggaccag cggatgccgg aacgtcacac ggtcgccgat cgcgaacagc ccgtctgttt
     4081 ccgcgccaac caccgcgacc gcgatcccga gtcgctcgga tgcccgcagc aacggcagtg
     4141 gatcgccgac tggctccgcc gccgcgacca gcaacaggcg ccgcgcgtcg tcagagagcg
     4201 cct
//
LOCUS       DASWBF010000091        59228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_9075, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000091 DASWBF010000000
VERSION     DASWBF010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 59228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59228
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9075"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VF874_02895"
     CDS             complement(<1..447)
                     /locus_tag="VF874_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX9175067.1"
                     /translation="MTDFRSGFVCFVGRPNTGKSTLTNALVGAKVAITSDRPQTTRHT
                     IRGIVHRDNFQIVLVDTPGLHRPRTLLGKRLNDLVRNTYSEVDVICLCIPADEEIGPG
                     DRWIVEQIRAVAPNTTLVVVVTKIDRVSKAQVVQQLTAVSELVDDAA"
     gene            complement(522..830)
                     /locus_tag="VF874_02900"
     CDS             complement(522..830)
                     /locus_tag="VF874_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX9175068.1"
                     /translation="MNDEDAKLVVLARGAMARADASTGAAVRDADGRTYAAAPVDLSA
                     LKLTALQAAVAAAVSSGATALEAAVLLGGDADDAGVAAVRELSPDAAIIVTDRAGNVV
                     "
     gene            complement(827..2116)
                     /locus_tag="VF874_02905"
     CDS             complement(827..2116)
                     /locus_tag="VF874_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9175069.1"
                     /translation="MTGIGALVGAIVLIALGGLFAAIDAALSTVSMARVEELVRDERP
                     GAVRLARVMAERPRYVNLIVLLRITCEAAATVLLVAFLWDDLGLKWGLVVAAALMVVI
                     SFVAIGVAPRTLGRQHAYSIALASAVPLQAISVLLTPISRLLVLLGNALTPGRGFRNG
                     PFASEIELREVVDLAQQRGVVADDERRMIQSVFELGDTPAREVMVPRTEMVWIESDKN
                     AGQATSLAVRSGHSRIPVIGENVDDVVGVVYLKDLVQQTYYSRNGGRDTKVTEVMRPA
                     VFVPDSKPLDALLREMQRNRNHMALLVDEYGAIAGLATIEDVLEEIVGEIADEYDTDE
                     VAPIEDLGDKNFRVSARLPIEDVGELYGVEFEEDLDVDTVGGLLALELGRVPLPGAEV
                     VSHGLRLRAEGGHDHRGRVRIGTVLVSPDKRKGNGQE"
     gene            complement(2113..2649)
                     /gene="ybeY"
                     /locus_tag="VF874_02910"
     CDS             complement(2113..2649)
                     /gene="ybeY"
                     /locus_tag="VF874_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX9175070.1"
                     /translation="MSIEVANESGLDVSEDELISVARFVIDKMNVHPAAELSMVLLDL
                     NSMADLHMRWMDLPGPTDVMSFPMDELEPGGRPDAPEPGPAVLGDIVLCPEFAAEHAA
                     AAGHSLGHELALLTIHGVLHLLGFDHGEPAEEKEMFALQRQLLEEWVAEQVKAYQQDR
                     QTEKDRRLLDKSRYFDEP"
     gene            complement(2649..3671)
                     /locus_tag="VF874_02915"
     CDS             complement(2649..3671)
                     /locus_tag="VF874_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9175071.1"
                     /translation="MTPRETSAAPTVRSSIDVPPDLIVGLLGSADENLRAMERVLSAQ
                     LHARGNTVTLSGSPADVALAERVVTELIALVAGGQVLTPEVVRHSIAMLTGDANESPA
                     EVLSLDILSRRGKTIRPKTLNQKRYVDAIDANTIVFAIGPAGTGKTYLAMAKAVNALQ
                     TKQVTRIILTRPAVEAGERLGFLPGTLYEKIDPYLRPLYDALHDMMDPEVIPKLMSGG
                     VIEVAPLAFMRGRTINDAFIILDEAQNTTPEQMKMFLTRLGFGSKIVVTGDVTQVDLP
                     SGATSGLRAAVGILDGIDDIHVAELTSADVVRHQLVSEIVDAYARAEDSSLSMNRAQR
                     RAHRTR"
     gene            3831..5051
                     /locus_tag="VF874_02920"
     CDS             3831..5051
                     /locus_tag="VF874_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9175072.1"
                     /translation="MTTSRLVFDPFSQEFFDNPYEIYRRMRDEEPVYYDEERDFYALT
                     RHEDVAAAFKNFETFSSAYGLDLAMVKSGGPPLDIKLLQLMDPPQHRHMGSVLNKMFT
                     PRAVQAQESMITATIDKYLDAIDGDEFDAVQQLTAPFPVEVITAMLGVPDEYVQQVRL
                     WVDATLHREPGQIEMGAAGLQAVVEQMAFYHSLIQEHRDQPRDDLFNMLIEAEIERDD
                     GGKSHLSDIEIAGFATQLGGAGAETVTKLLGNAVYLFARNPDQWQKLLDDRSKIPAAV
                     EELLRFEPPAQYDVRCSIREITVRGVTIPPRKPVFLLIGSANRDPRAWTDVDTFDIDR
                     NRTEAQNLGLGYGIHSCLGAALARMESAIALEKLPDFMPRYEVDWDGCRRVHMQNVIG
                     WSNVPVRIIREPTA"
     gene            complement(5029..5766)
                     /locus_tag="VF874_02925"
     CDS             complement(5029..5766)
                     /locus_tag="VF874_02925"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX9175073.1"
                     /translation="MGGPLFYVDALPAEGAVATVSGDERFHAATVRRIRPGEQLLLGD
                     GAGELASCEVSDVDRGALHARVLSRWSVPPAQPSVTVVQALPKAERSELAIESATEAG
                     ADEFVAWQAARCVARWDSARAEKGLRRWRAVARSAARQSRRAHIPVVSGPVSTAELLP
                     RIADDTTLVLHESAAEVLSEKHVAQADSIVLVVGPEGGIPDEELEALTTAGAVAVRLG
                     PTILRTSTAAAVALGALGVLTTRWARE"
     gene            complement(5768..6910)
                     /gene="dnaJ"
                     /locus_tag="VF874_02930"
     CDS             complement(5768..6910)
                     /gene="dnaJ"
                     /locus_tag="VF874_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9175074.1"
                     /translation="MARDYYALLGVSRNASDAEIKRAYRKLARELHPDVNPDEGAQAR
                     FKEISVAYEVLSDPEKRRIVDLGGDPMENAATSGNGFGTGFGGLGDVFEAFFGGGSAS
                     RGPIGRVRPGADSLLRLRLDLEECATGVTKQVTVDTAVLCDKCQGKGTNDNSNPVRCD
                     TCGGRGEVQSVQRSLLGQVMTSRPCPTCRGVGEVILDPCYRCGGDGRVRARREISVKI
                     PAGVGDGMRIRLAAQGEVGPGGGPAGDLYVEVHEQQHDVFIRDSDDLHCTVSVPMVDA
                     ALGTDVTVDAILDGPTEVKLPGGTQPGSVVTLRGHGMPHLRTGVRGDLHVHVEVTVPT
                     RLDHHDAELLRELKKRRDDVAEVKSPHAAGGGGGFFSRLRETFTGR"
     gene            complement(6921..7952)
                     /gene="hrcA"
                     /locus_tag="VF874_02935"
     CDS             complement(6921..7952)
                     /gene="hrcA"
                     /locus_tag="VF874_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX9175075.1"
                     /translation="MGHAEDRRFDVLRAIVADFVATKEPIGSKTLVERHNLGVSSATV
                     RNDMAVLEAEGYIAQPHTSSGRIPTEKGYREFVDRLDDVKPLSTAERRAIQGFLESGV
                     DLDDVLRRAVRLLAQLTRQVAVVQYPTLSSSTVRHVEVVALTPARLLLVVITDSGRVD
                     QRVVELGDVIDDHQLAQLRDMLGQALDGKRLSAASAAVADLASEIGPKGTLGDAVGRS
                     ATVLLESLVEHAEDRLLLGGTANLTRNTADFGGSLRSILEALEEQVVVLRLLQAQQQA
                     GKVTVRIGHETEVEEMAATSVVTTTYGADGDVYGGMGVLGPTRMDYPGTIASVAAVAM
                     YIGDVLGRR"
     gene            8137..8451
                     /locus_tag="VF874_02940"
     CDS             8137..8451
                     /locus_tag="VF874_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9175076.1"
                     /translation="MIFKGVRDGKPYPDHGLSYRDWAQIPPRQIRLDELVTTTTVLAL
                     DRLLSEDSTFYGDLFPHAVKWRGVVYLEDGLHRAVRAALRNRVVLHARLLDMDELQAQ
                     RA"
     gene            complement(8448..9134)
                     /locus_tag="VF874_02945"
     CDS             complement(8448..9134)
                     /locus_tag="VF874_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEX9175077.1"
                     /translation="MSAERQFSMYGPSHWAVLAVFVIGAVALVWAGRRQSESQARLLG
                     RILGALTFAVYGAALMYSVFDPRIPPPVPLRLTDLATVAAAYALWSQRHWAFALTYYW
                     GLVLSAQALVSPVLIGLDFPSLHFLAFWSIHLLVVWAAIYLTWGRGMRPTWRSYRVAA
                     VTTAIWAAVTFTYNRIAGTNYGFLNRKPATASMLDVLGPWPVYIFTAAALILAVWALM
                     TWPWQRNRPS"
     gene            complement(9140..10276)
                     /gene="hemW"
                     /locus_tag="VF874_02950"
     CDS             complement(9140..10276)
                     /gene="hemW"
                     /locus_tag="VF874_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX9175078.1"
                     /translation="MTRTPDRQFGVYVHVPFCATRCGYCDFNTYTPAELGGANPDAWL
                     EALRAELRLAAELAGAQPVDTVFVGGGTPSVLGADRLAAVLDAVRENFGLVPDAEVTT
                     EANPESTSPELFDALLAAGYTRMSLGMQSVTPHVLRVLDRVHSPGRAVAAVEEALAAG
                     FRHVSLDLIYGTPGESDDDLLRSVDTAVEAGVDHVSAYALVVEDGTALARRVRRGEVD
                     APDDDVLARRYELLDAQMADAGFDWYEVSNWARPGGECRHNVGYWNGGEWWGAGPGAH
                     GFVGSTRWWNIKHPNAYAAALAEGALPVAGFEELGPVETHTETVMLSIRMRSGLPAAA
                     LDDAEMARAQTAIADGLLVRDADRLVLTDRGRLLADGVVRTLLG"
     gene            10651..12312
                     /locus_tag="VF874_02955"
     CDS             10651..12312
                     /locus_tag="VF874_02955"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX9175079.1"
                     /translation="MTRPTTKRKGEGQWALGYREPLNANEQSKKDDAPLNVRARIENI
                     YALGGFDSIDKSDLRGRFRWWGLYTQREQGYDGTWTGDEHIETIEAKYFMMRVRCDGG
                     ALTVEQLRTIGAISTEFARDTADISDRENVQYHWIRVEDVPEIWRRLEAVGLQTTEAC
                     GDCPRVVLGSPLAGESLDEVLDASPAVDEIVRRYIGKPEYANLPRKYKTAISGLQDVV
                     HEVNDIAFIGVNHPEHGPGLDVWVGGGLSTNPMLGQRLGAWVPLDEVPDVWEAITSAF
                     RDYGYRRLRSKARLKFLIKDWGVERFREVVETEYLKRPLIDGPAPEPAVHPIDHVGVQ
                     RLKNGRNAVGVAPIAGRVSGTILSKVADIAENAGSDRVRFTPYQKLIVLDVPDDKLDE
                     AVSSLEALGLPSQPSHWRRNLMACTGIEYCKLSFAETRVRAQGLVPELEKRLEDINAQ
                     LDVPVTININGCPNSCARIQVADIGFKGQMVDDGDGPVEGFQVHLGGSLGLDSGFGRK
                     LRQHKVLSHDVGDYIERIVRNFVKQRSEGERFATWALRADEADLR"
     gene            12309..13010
                     /locus_tag="VF874_02960"
     CDS             12309..13010
                     /locus_tag="VF874_02960"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX9175080.1"
                     /translation="MSVTTETDLRELAERGAEELDGAATIDLLRWVDENFRDDYVVAS
                     NMQDAVLIDMAVKVRPGVDVLFLDTGYHFAETIGTRNAVSDVYDVNVINVTPEHSVPE
                     QDALLGKNLFARDPNECCRLRKVAPLGTALRGYSAWVTGLRRVEAPTRANAPVISFDA
                     AFGLVRVNPLAAWTDQDIQDYIDAYDVFVNPLVFDGYPSIGCAPCTARPVDGADPRSG
                     RWQGIGKVECGLHSE"
     gene            12989..13720
                     /locus_tag="VF874_02965"
     CDS             12989..13720
                     /locus_tag="VF874_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HEX9175081.1"
                     /translation="MRTAFRMSPAVLVLTAHGSADPRAAVNTHAVAEEIRRQRPRLDV
                     QVAFCEQSTPNLRDVVAELGDKSVVTPLLLADAYHARVDIPAMIAESGAAQADVLGED
                     DRLVTVLRQRVRSLGVSRLDSDVGVAVVAVGSSRRQANTRTAAVAPAFSAGTRWAGAV
                     TAFATGRRPSLDEATEQLRARGARRLVIAPWFLAHGRITDRVAEYAGAHGIPMAEPLG
                     AHRLVAATVLDRFDRARAATNPLAA"
     gene            complement(13717..14766)
                     /locus_tag="VF874_02970"
     CDS             complement(13717..14766)
                     /locus_tag="VF874_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEX9175082.1"
                     /translation="MTDAITLRGVNKHYGEFAALEDINFTVPAGSLTTLLGPSGSGKS
                     TLLRAIAGLDQPDTGTITINGRDVTRVPPQRRGIGFVFQHYAAFKHMNVRDNVAFGLK
                     IRKRPKAEIEDKVDGLLETVGLSGFQRRYPGQLSGGQRQRMALARALAVDPQVLLLDE
                     PFGALDAKVREDLRAWLRRLHDEVHVTTVLVTHDQSEALDVSDRIAVLNKGRIEQIGS
                     PTDVYDQPANGFVMSFLGTVSALNGILVRPHDIRVGRNPEMAVATSDGTAKSIGVMRA
                     TIDRIVYLGFEVRVELTAAANGAPFTAQITRGDAEALALKEGDTVYVRATRVPPIAGS
                     TQLPETEELEPAPTS"
     gene            complement(14788..15600)
                     /gene="cysW"
                     /locus_tag="VF874_02975"
     CDS             complement(14788..15600)
                     /gene="cysW"
                     /locus_tag="VF874_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX9175083.1"
                     /translation="MIPSPLVKYLSRSLALAYVLVLVVVPVGLILWRSFSPGLDEFFA
                     QITTPAAISALQLSLLVVAIVVPLNVLFGIPTALVLARSRFRGKGALQAIIDLPFAVS
                     PVVVGVALIVLWGSAGAFGFVERDLGFKIIFGLPGIVLASIFVTLPFVIREVEPVLHE
                     IGTDQEEAAATLGSKWWQTFWRITLPSIRWGLTYGVVLTIARTLGEYGAVIMVSSNLP
                     GTSQTLTLLVSDRYNRGAEYGAYAISTLLMTVAVLVLIVQVVLDARRARAEV"
     gene            complement(15597..16442)
                     /gene="cysT"
                     /locus_tag="VF874_02980"
     CDS             complement(15597..16442)
                     /gene="cysT"
                     /locus_tag="VF874_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX9175084.1"
                     /translation="MTAALDPKPQATATRGLGFRGTTSLRIGVATLWLSVIVLLPLAA
                     IVWQSAKGGWSAFVTAVTSPSALDSFRVTLTISAAVTVINVFFGLLVAWVLVRDDFPG
                     KRLVDAVIDLPFALPTIVASVVLLALYGPNSPVGIHLQHTQYGVGVALLFVTLPFVVR
                     AVQPVLMEVDHEAEQAAASLGASNATIFRTIVFPALTPALLSGAGLAFSRAIGEFGSV
                     VLIGGAVPGKTEVSSQWIRTLIENDDRTGAAAISIVLLALSFVVLLILRALGSRAAKR
                     EELAK"
     gene            complement(16439..17476)
                     /locus_tag="VF874_02985"
     CDS             complement(16439..17476)
                     /locus_tag="VF874_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9175085.1"
                     /translation="MLRFPKVLRLRHAAALALTATVLAACGGGASDVAGSNGPSNARA
                     TLTLVAYAVPEPGWSKVIPAFNATEQGKDIAITTSYGASGDQSRSVVDGKPADIVNFS
                     VEPDITRLVKAGKVSENWNSDVTKGIPFGSVVALVVRQGNPKNIQDWDDLLRPDVEVI
                     TPSPLSSGSAKWNLLAPYAAKSNGGQDQQAGLDFVQQLVSQHVKLRPGSGREATDVFL
                     QGSGDVLISYENEAINVERQGKPVQHLIPPQTFKIENPLAVVNTTTHLQEATDFKNFQ
                     YTEEAQRLWAQAGFRPVNPGVAQQFANDFPAPAQKLWTVAELGGWSTVDSSLFDKDNG
                     LITKIYKQATG"
     gene            18002..18412
                     /locus_tag="VF874_02990"
     CDS             18002..18412
                     /locus_tag="VF874_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9175086.1"
                     /translation="MRFVDTNVLLYAISRDPEERDKAQRANEILTARDLALSVQVLQE
                     FYVQATRTSRPDAITHDQAVALVESFMRFPVATITAELMLAAVATHWRFGVSYWDAAI
                     LEASRFLGCDVVLSEDLSNGEDYGGVRVQNPFSG"
     gene            complement(18485..18676)
                     /locus_tag="VF874_02995"
     CDS             complement(18485..18676)
                     /locus_tag="VF874_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175087.1"
                     /translation="MLGFLNWWDGVELWLSGLGFVLQTIVVMPVVLLLAYGLAVLLDG
                     ALGRGIGLLRRIRHGEPGE"
     gene            18763..20004
                     /locus_tag="VF874_03000"
     CDS             18763..20004
                     /locus_tag="VF874_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9175088.1"
                     /translation="MPASADSTTCVIAGGGPAGMVLGLLLARGGVPVVVMEKHADFLR
                     DFRGDTVHASTLRLLDELGLGPRFAQVPHRLIESIRMQVQDVPISLGLNRIPGAHKHI
                     ALVPQWDFLELLATAAEEEPTFTLMRSTEVHSALVRDGRVAGVRYRGADGETREMRAA
                     LTVACDGRSSTLRSSMGLQPKAFGAPMDVWWFRLPRQSGDPTGLNGVFRSGHACVVID
                     RGDYYQIAYIIPKGTDSAMRAEGVAALRRQLVQLVPWLSDRVEQIASMDEVKLLDVQL
                     NRLHRWYTDGLLLIGDAAHAMSPVGGVGINLAVADAVAAAAALAAPLREGNLGKRQLA
                     RVQARRWLPTVLIQSAQRLIHARVIAVAMSEAAAESPKPPLPVRMMSKLPALGAVAGY
                     AIAIGPLPEHAPAFARRDAPM"
     gene            20057..22048
                     /locus_tag="VF874_03005"
     CDS             20057..22048
                     /locus_tag="VF874_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX9175089.1"
                     /translation="MVLSAEPAEQTPEAPFSATSPVPYIQTSSTRNPFPPIADYGFLS
                     DCENTCLISSSGSVEWLCVPRPDSPSVFGAILDRAAGHFRLGPYGVSVPAARRYLPGS
                     LIMETTWQTHTGWVIVRDALVMGPWHDLETRSRTHRRTPMDWDAEHIMLRTVRCVSGT
                     VEMVLNCEPSFDYHRISATWEYSGPAYGEAIARAKRDPDSHPQLKLTTNLRIGLEGRE
                     ARARTRLKEGDNVFVALSWSKHPAPQTYEEAADKMWKTSECWRQWINIGDFPDHPWRS
                     FLQRSALTLKGLTYSPTGALLAAATTSLPETPHGERNWDYRYAWVRDSTFALWGLYTL
                     GLDREADDFFSFIADVSGANNGERHPLRVMYAVGGERTLVEEELHHLSGYDGARPVRI
                     GNGAYNQRQHDIWGTMLDSVYLHAKSREQIPESLWPVLKNQVEEAIKHWREPDRGIWE
                     VRGEPQHFTSSKVMCWVALDRGAKLAELQGEKSYAQQWRAIAEEIKADVLKHGVDSRG
                     VLTQRYGSDALDASLLLVVLTRFLPPDDPRVRNTVLAIADELTQDGLVLRYRVEETDD
                     GLSGEEGTFTICSFWLVSALVEIGEVSRAKHLCERLLSFASPLHLYAEEIEPRTGRHL
                     GNFPQAFTHLALINAVVHVIRAEDEGDSAGVFVPANAPM"
     gene            complement(22051..22761)
                     /locus_tag="VF874_03010"
     CDS             complement(22051..22761)
                     /locus_tag="VF874_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX9175090.1"
                     /translation="MSNFAALPRVCAIVAAAGIVLSGCTQSIGGTAVKGPGPAGSNVP
                     PLAESGLDQILLSIDDVGSIVGSSDLQLSSSSEDLADNSDSIDKSECLGVFYSAEQQI
                     YDKSGWKAVRDQIIREPGDNKKHWVEQTVVLFANADKAANFLDKSRDEWKKCQQSSAT
                     TEGSDTSYNWDFGRLQEPSETMMSMDMDQQDSNNWGCQHAMGTVSNIIVEGVACANGV
                     GDEGRQVVEQIVKNASAK"
     gene            complement(22804..24684)
                     /gene="lepA"
                     /locus_tag="VF874_03015"
     CDS             complement(22804..24684)
                     /gene="lepA"
                     /locus_tag="VF874_03015"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX9175091.1"
                     /translation="MELSPLPPHQEIPISNFADQTFTAPAQIRNFSIIAHIDHGKSTL
                     ADRMLQLTGVVDERSMRAQYLDRMDIERERGITIKAQNVRLPWTVGDQDYVLHLIDTP
                     GHVDFTYEVSRALEACEGAVLLVDAAQGIEAQTLANLYLALDRDLVIIPVLNKIDLPA
                     ADPDRYAAEIAHIIGCEPGDVLRVSGKTGDGVAELLDHVVREVPPPTGDSDAPTRAMI
                     FDSVYDTYRGVVTYVRVIDGKIVPRERIKMMSTGATHELLEVGIVSPEPKPADGLGVG
                     EVGYLITGVKDVRQSKVGDTVTTARRGATDALTGYREPKPMVYSGLYPVDGSDYPDLR
                     DALDRLQLNDAALTYEPETSVALGFGFRCGFLGLLHMEISRERLEREFDLDLISTSPN
                     VVYRVVLEDDSEKIVTNPSDWPSGKIRTVYEPVVKTTIISPSEFIGTIMELCQSRRGE
                     LGGMDYLSPERVELRYTMPLGEIIFDFFDALKSRTRGYASLDYEEAGEQEAELVKVDI
                     LLQGEPVDAFSAIVHRDAAAEYGRKMTGKLKELIPRQQFEVPVQAAIGSKIIARENIR
                     ALRKDVLSKCYGGDITRKRKLLEKQKEGKKRMKTIGRVDVPQEAFVAALSTDAGGDKP
                     KK"
     gene            24714..25274
                     /locus_tag="VF874_03020"
     CDS             24714..25274
                     /locus_tag="VF874_03020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX9175092.1"
                     /translation="MASPWKTLQRRLGKAAENLVFNEGPKFISQGIRLGLEAIIRGPE
                     PAREITAGRPVTKNSVPTAYRARTIVYSPDLDGRADPGEIVWTWVVYEDDPSKGKDRP
                     VLVVGRDRSMLLGLMLSSQGHHADDPNWVGIGAGSWDYDGRASWVRLDRVLDVPEEGI
                     RREGAILGHEKFEIVAARLRAEYSWS"
     gene            complement(25345..25470)
                     /locus_tag="VF874_03025"
                     /pseudo
     CDS             complement(25345..25470)
                     /locus_tag="VF874_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214647.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            25512..25799
                     /locus_tag="VF874_03030"
     CDS             25512..25799
                     /locus_tag="VF874_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175093.1"
                     /translation="MHEMVHVALFARDIGNFDGQRHHNTDVWCEEIIRITPQLGLPPI
                     KAAPVKPRRIDGKVARRALEGHISRDDMAHWPHSLRPAGFYNREGRIHVPI"
     gene            26002..26337
                     /locus_tag="VF874_03035"
     CDS             26002..26337
                     /locus_tag="VF874_03035"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9175094.1"
                     /translation="MMFTRHDITKLVGTAIAAAALGLTTAGSAAADSTDDAFLHRVAA
                     DGLMFSLQEAVIQRAHVVCEAFSTGASPASVHATMLNNSAMSPRQTALFMADAVQAYC
                     PKYADLLFS"
     gene            complement(26474..26581)
                     /locus_tag="VF874_03040"
                     /pseudo
     CDS             complement(26474..26581)
                     /locus_tag="VF874_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921411.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(26578..27006)
                     /locus_tag="VF874_03045"
     CDS             complement(26578..27006)
                     /locus_tag="VF874_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9175095.1"
                     /translation="MRIADVLRNKGSAVATVEPHVTVTELLAGLAQQNIGAMVVVDSD
                     AVVGIVSERDIVRKLNEHGADLLAQPVSAIMTVVAVTCTPRDAVDLLSGLMTENRVRY
                     VPVIQDGKLAGIVSTGDVVKTRMDELGAEREQLQTYITRG"
     gene            27116..27952
                     /locus_tag="VF874_03050"
     CDS             27116..27952
                     /locus_tag="VF874_03050"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX9175096.1"
                     /translation="MIEVILLGTGSPIPDPQRAGPSTLVRAGSQTLLVDCGRGVLQRA
                     AAVGVGANALSALLLTHLHSDHIADLGDVLITRWVTTFTPNPSPLPIIGPPGTASVVD
                     AMLDAFGPDIGYRIAHHADLTAPPAVEVEETTQGEVWARDGVRITVAPTDHRPVAPTI
                     AFRIESGGASVVLAGDTVPCDSLDELAAGANALVHTVIRKDLVESLPQQRARDICDYH
                     SSVEEAAATAARAGVDTLVLTHCVPAVAPGQEDEWRARAASAFGGRVELGPDLHRVEV
                     GA"
     gene            complement(28071..28910)
                     /locus_tag="VF874_03055"
     CDS             complement(28071..28910)
                     /locus_tag="VF874_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9175097.1"
                     /translation="MWRLRVVHSTGYAYKSPATSSFNEARLTPRSDNRQNVILSRVET
                     VPATRSYRYIDYWGTAVTAFDLHAPHTELEVTASSVVETDKPEPPSEKVTWDELNTEA
                     VIDRFDEVLTPTHYTPHSRRLGRVGERIVRDYDPYDAVIAASGWVQSELDYVAGTTGV
                     HSSALDALREGKGVCQDFAHLTLVLLRSMGIPSRYVSGYLHPHRKATVGETVDGQSHA
                     WIQAWTGGWWNFDPTNDKEINEQYVTVGLGRDYADVSPLKGIYSGGGSTDLDVVVEIT
                     RLA"
     gene            complement(28942..29919)
                     /locus_tag="VF874_03060"
     CDS             complement(28942..29919)
                     /locus_tag="VF874_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX9175098.1"
                     /translation="MLARNAEALYWIGRYVERADDTARILDVTVHQLLEDSSVDPDHT
                     SRVLLRVLGVAPPNSPLNVWSLTDLVAFTRGLEDGCSIVDAISAARENARSAREVTST
                     ELWECLNTTYNALPERERAAKRLGPHEFFSYIEGRAAMFAGLADSTLSRDDGYRFMVL
                     GRAIERVDMTVRLLLSRVGDSASSPAWVTLLRSAGAHDTYLRTYRGVLDATRVVEFML
                     LDRLFPRSVFYSLRLAEHSLDELDHRPHSRVGATAEAQRLLGRARSELEFLRPGVLLE
                     SLEERLGAIQQTCRELGGALAQQYFHSAPWVAWSDAGRNGSLVIEEGEI"
     gene            complement(29937..31454)
                     /locus_tag="VF874_03065"
     CDS             complement(29937..31454)
                     /locus_tag="VF874_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011594354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX9175099.1"
                     /translation="MFDNFDTVRAPYKGIYTELAPSDASELAARSDALGRAFIDQGIT
                     FSLSGQERPFPLDLVPRVISAPEWSRLERGITQRVKALEMYLDDIYGEQNILRDAVIP
                     RRLVTSCKHFHREAAGINPPNGVRIHVAGIDLVRDADGTFRVLEDNLRSPSGVSYVME
                     NRRTMARVFPNLFATNRVRTVGDYSSHLLRALRNSAATNEADPTIVVLTPGVYNSAYF
                     EHSLLARQMGVELVEGRDLFCRDNQVYMRTTEGERQVDVVYRRIDDDFLDPMQFRPDS
                     VLGVAGLLNAARAGNVVISSAVGNGVGDDKLIYTYVPTIIEYYLGEKPLLANVDTFRC
                     WLEDEREEVLDRIDELVIKPVEGSGGYGIVFGPDASERELAATAKKIRNDPRGWIAQP
                     VVQLSTVPTQIGDRLAPRHVDLRPFAVNDGDDVWVLPGGLTRVALTEGSLVVNSSQGG
                     GSKDTWVLASRASAPDRELAAAEVVRKLPRESRVPAATEMGPGLTSSQQQQQQQQ"
     gene            31705..31965
                     /gene="rpsT"
                     /locus_tag="VF874_03070"
     CDS             31705..31965
                     /gene="rpsT"
                     /locus_tag="VF874_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9175100.1"
                     /translation="MANIKSQEKRNRTNERRRLRNKSVKSSLHTAIRAFREAAESGDK
                     DKATVLARAASRKLDKAASKGVIHKNQAANRKSSLTLTLNKL"
     gene            complement(31967..32914)
                     /locus_tag="VF874_03075"
     CDS             complement(31967..32914)
                     /locus_tag="VF874_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX9175101.1"
                     /translation="MHLVLGDEELLVERAIAAVLRDARAQAGTDDVPESRLRAGEVTT
                     SELAELLSPSLFADERVVVLDAAAEAGKDAAALISSAAADLPHGTVLVVVHSGGGRAK
                     ALADELRKLDAHLHPCARITKAGERAEFVRGEFREQRLDVDDDVVSVIIDAVGSDLRE
                     LAAACSQLIADTGGDVDVSAVRRYYSGRAEVKGFDIADKAVVGDVAGAVEALRWAMIR
                     GEPRVVLADALAEAVHTIARVGPLSGDPYRLASELGMPPWRVKKAQRQARGWSTQSVA
                     EAMRLVATLNADVKGAAADADYVLEATTRRIAELVGRRR"
     gene            complement(32922..34367)
                     /locus_tag="VF874_03080"
     CDS             complement(32922..34367)
                     /locus_tag="VF874_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX9175102.1"
                     /translation="MTAIAIVWRAGTAAAVVCVLVAASAAVAGWRARRQGAPVRAAVS
                     AVVLGAAVIGAGFGWAGAVRVSALEHHPLAALYSRSTDVTVTASEQPRPVRDGGRVVF
                     RAALRRIGGDDASGRVTVFAPARGFGELAPGRPASFRALVGRPTRRDLSVAVLSASGT
                     PTFGEASPVQRAAESVRRRFADAVRSQLPADQAAALPALVLGDTSTLPPETVAAFRAA
                     GLTHLTAVSGANVTIVCGAVLLTARFVGPRVAVALAALVLVGFVIVVQPSASVLRAAV
                     MGAIGLLGVLTHRRRHAMPALSATVIALMIVAPEMSVDLGFALSVSATAALIVIAPMW
                     SGRLVARGWPKPVADAVCVATAAQVVTAPLIAAISGRISVVSIVANVAVTAVIPPITV
                     AGTAAAALAPLWPGAAHLLIRFTGPELWWLLNIARWAGGVPGATVEVPSRLPGMVLVA
                     AGALGTVLLWRLVARARERRRPSMARSSSEL"
     gene            complement(34428..35246)
                     /locus_tag="VF874_03085"
     CDS             complement(34428..35246)
                     /locus_tag="VF874_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEX9175103.1"
                     /translation="MRTELPAERLHRRLNSEPDGTADESDSQDTSLSRWLPSASQDTG
                     PAWLAAVRADPGRAGLFALAAVGVIAVIVTAFAMMRDKPPAVVSAKLPPVQMVSSAAP
                     SPSATPSTAIADGPVVVSVVGLVHKPGLVTLQPGARIADAVTSAGGTLDGADLIGLNM
                     AQRVADGEQIVVGIAPAPGEPTKLGSGVTPSSGGPGSPGSSTSSSGPAAGPIDLNSAT
                     VEQLDTLPGIGPVTAAAIVAWRDANGKFTSVDQLADVDGIGPTRLEKLRSLVRV"
     gene            35385..36443
                     /locus_tag="VF874_03090"
     CDS             35385..36443
                     /locus_tag="VF874_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEX9175104.1"
                     /translation="MTSQPARPLRVIQWTTGNIGQRSLHAIIGRPDMELVGVYAHGVE
                     KAGKDAADLCGWPEPTGVTATNDIDALIALRPDACCYNPLWPSVDELVRLLEAGVNVC
                     SSAAWITGGKQTPEDRERIEKACVQGNSTMFGSGAHPGMSNLVGMVLSGSCERVDEIR
                     ITESVDCSTYESAGTQKAMGFSQPPDTPGLAESVRRESEVFAESAAMMADAMGATLDK
                     MTFDVTFTAATDESDLGFMQIPKGTVAGVYGYHRGWVGDRNVVSVGFNWIMGYHVDPP
                     KPLEHGHVVQVFGLPNMRAVIHCLPPKDWTEPGFMGLGMIYTAMPVTNAVPKVVAAQP
                     GIVTLADLPPITGRPPTP"
     gene            complement(36418..37269)
                     /locus_tag="VF874_03095"
     CDS             complement(36418..37269)
                     /locus_tag="VF874_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX9175105.1"
                     /translation="MAVVVVTDSSSRLCDGDREQWGIRQVPLHILCDGEDFRDGVDAI
                     PVDIHSRPKTTTAGATPAELSTAYRQALEDSSGDGVVAVHVSAALSSTVTSGEQAARQ
                     FSGAVRVVNSKSVALATGFVVLAAARAAAEGCDLDSVEAQALSAVGRVRGYVVAQRLD
                     NLRRGGRIGTAASWLSTALAIKPVLRIDPDGRLVLAQRVRTASKALDALVEQVVTEVG
                     ERSAIVAVQHVDNLSAANDVAHELIARLHPAEPPTVADMGPVLGVHVGAGAVAVCVEI
                     TESADDP"
     gene            complement(37275..38021)
                     /gene="octT"
                     /locus_tag="VF874_03100"
     CDS             complement(37275..38021)
                     /gene="octT"
                     /locus_tag="VF874_03100"
                     /EC_number="2.3.1.273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016414 - O-octanoyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosylglycerate octanoyltransferase"
                     /protein_id="HEX9175106.1"
                     /translation="MLSERAAHRQVLLIFADSLAYYGPTGGLPADDPRIWPNIVAAQL
                     GWDVELIGRIGWTCRDVWWAATQDPRAWAALPRAGAVIFATSGMDSLPSPLPTALRES
                     IRYVRPAWLRRWVRDGYCWLQPRLSPIARSALPPHLSVEYLEMTRNAVDFNRPGIPIV
                     ASLPSVHIADTYGRAHHGRNGTVDAITEWAGQHGVPLVDLKAAVVEHVMSGRGNPDGI
                     HWNFEAHQAVAELMLNALTEAGVTQENRQG"
     gene            complement(38011..38679)
                     /gene="gpgP"
                     /locus_tag="VF874_03105"
     CDS             complement(38011..38679)
                     /gene="gpgP"
                     /locus_tag="VF874_03105"
                     /EC_number="3.1.3.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate phosphatase"
                     /protein_id="HEX9175107.1"
                     /translation="MTVRQLVMVRHGQTEFNAGSRMQGQLDTELSDLGRAQAVAVAEV
                     LGKRHPLLIVSSDLRRAYETAVVLGERTGSPLVVDERLRETHLGNWQGMTHGEVDAAA
                     PGARVEWRNDATWVPHGGESRIDVARRSSPVVAELVESQSDWGADEPDRPLVLVAHGG
                     LIAALTAKLLGLPADNWPVLGGMGNASWVQLSGHSAAEASLEDIRWRLDVWNASAQVA
                     DDAL"
     gene            complement(38676..39050)
                     /gene="rsfS"
                     /locus_tag="VF874_03110"
     CDS             complement(38676..39050)
                     /gene="rsfS"
                     /locus_tag="VF874_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX9175108.1"
                     /translation="MTASAEATEMATIAARAAAAKLADNVVVLDVSEQLVITDAFVIA
                     SASNERQVNAIVDEVEEKMRRAGYKPARREGAREGRWTLLDYVEIVVHIQHEDERDFY
                     ALDRLWRDCPEIPVDLDGDGTS"
     gene            complement(39047..39652)
                     /gene="nadD"
                     /locus_tag="VF874_03115"
     CDS             complement(39047..39652)
                     /gene="nadD"
                     /locus_tag="VF874_03115"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX9175109.1"
                     /translation="MMGGTFDPIHNGHLVAASEVADRFHLDEVVFVPTGEPWQKHGRH
                     VTPAEDRYLMTVIATASNPRFSVSRVDIDRGGRTYTRDTLRELHELHPDSELYFITGA
                     DALASILTWQDVEELFSLARFVGVSRPGYDLDGAHVSAAFKSLPSDALTLLEVPALAI
                     SSTDCRARAGENRPIWYLVPDGVVQYVAKRGLYRCASQDKS"
     gene            39828..40061
                     /locus_tag="VF874_03120"
     CDS             39828..40061
                     /locus_tag="VF874_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9175110.1"
                     /translation="MRATIDKAGRLVIPKQLRDHPGLRPGKVEVTADGAGLRVEPLID
                     DSLGERDGRLVIPAHGVEINDQVVRILRDATQR"
     gene            complement(40109..41542)
                     /locus_tag="VF874_03125"
     CDS             complement(40109..41542)
                     /locus_tag="VF874_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9175111.1"
                     /translation="MAARRVRPPQPLAPHGIPGHLVGFVEALRAQGISVGPSETVDAG
                     RVMSVLGLGDREVLREGIACAVLRRPDHRDTYDAMFDLWFPAALGARATVPIEDEDSS
                     GSPDDLDALRAQLVELLGDDSDLGDHDERMEALVAQIVEAYGRYSSSRGPSYSSYQAL
                     KAMALDQLEGRLLAGLLAPYGEEPTPTQEQIAKAIASQRISQLRKMVEAETKRRTAEQ
                     LGRDHVAMYGIPQLSENVEFLRASGEQLRQMRRVVAPLARTLATRLAARRRRSRAGEI
                     DLRKTLRKSMNTGGVPIDMVLRKPRPARPELVVLCDVSGSVAGFSHFTLLLVHALRQQ
                     FSRVRVFAFIDTTDEVTHMFGPEADLAMAVQRITRDSGVYTRDGHSDYGNAFTSFTEN
                     YPTVLSPRSSLLVLGDGRNNYRNPEVDVLHRMVTESRHAHWLNPEPRHLWGSGDSAVP
                     RYQDVITMHECRNAKQLAEVIDGLLPV"
     gene            complement(41542..42417)
                     /locus_tag="VF874_03130"
     CDS             complement(41542..42417)
                     /locus_tag="VF874_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9175112.1"
                     /translation="MSVPARPAPLFADIDDVARRLAETGYLPDTATATAVFLADRLGK
                     PLLVEGPAGVGKTELARAVAQATGSGLVRLQCYEGVDESRALYEWNHAKQILRIQAGS
                     GDWEHTKTDVFSEEFLLSRPLLTAIRRTEPTVLLIDETDKADIEIEGLLLEVLSDFAV
                     TVPELGTIVAERAPLVVLTSNATRELSEALKRRCLFLHIDFPDPDLERRILLSRVPEL
                     PEHLAEELVRIIGVLRGMQLKKLPSVAETIDWGRTLLALGLDTIDDATIASTLGVVLK
                     HQSDVTRAAGELRLN"
     gene            complement(42433..43680)
                     /locus_tag="VF874_03135"
     CDS             complement(42433..43680)
                     /locus_tag="VF874_03135"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX9175113.1"
                     /translation="MSLQAPSRPDLREHVHDAARRARVASRLLATLSTADKDRALHAA
                     ADALLSHMHVILAANAEDLDAARAAGTSEALLDRLALNPHRVDGIAAGLRQVAGLPDP
                     IGEVLRGRTLPNGLQLRQQRVPFGVVGIVYESRPNVTVDAFGLTLKSGNAVLLRGSSS
                     ASHSNTALVNVLRAALATENLPPDAAQLLSAEDRSTVTHLIQARGLVDVVIPRGGAGL
                     IDAVVRDAQVPTIETGVGNCHVYVHEAADLDVAERILLNAKTRRPSVCNAAETLLVDA
                     AIAGEALPRLTNALESAGVTIHSGGAEDDLRREYLSMDIAVAVVDGLDAAINHINEYG
                     TGHTEAIVTSNLAAAQRFTEEVDAAAVMVNASTAFTDGEQFGFGAEIGISTQKLHARG
                     PMGLPELTSTKWIVWGDGHTRPA"
     gene            43737..44591
                     /locus_tag="VF874_03140"
     CDS             43737..44591
                     /locus_tag="VF874_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9175114.1"
                     /translation="MTRVCVVGSVNTDLTFTVDALPRAGETVLSSALASSPGGKGANQ
                     ALAAARAGARVQFVGAVGTDAAADALLAHLRSNDVGTDAVITVPGPSGTAAVLVDADA
                     ENMIVVAAGANAHLVLDSAFMRDVVAQSDVVLLQLEIPAPTAVAAARVARTANATVIV
                     NASPPSTSPEMPGLAALADVVVVNESEAGAWRWAVPHLVITQGARGATYIADSHEVDV
                     AAPPVEPVDTTGAGDVFAGVLAAGWPGDPDRALRRACAAGALATLVPGAGDCAPTNEA
                     IEDALRGC"
     gene            44591..45220
                     /locus_tag="VF874_03145"
     CDS             44591..45220
                     /locus_tag="VF874_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX9175115.1"
                     /translation="MSLSETRRRTLGRRIRLLVAATISYNVVEAMIAVAEGIRVSSSA
                     LVGFGLDSVVEVSSAAAVAWQFSAKDPESREKPALRFIAWSFFALAAYVTVDAVLSLI
                     GIGEPRPSAIGIGLAAVSLIVMPLLSFAQRSAGRELGSRSAVADSKQTLLCTFLSAAL
                     LLGLVANAVAGWWWADPVAALLIAAVAVREGREAWRGDPCCTAHVEELQ"
     gene            complement(45715..47757)
                     /locus_tag="VF874_03150"
     CDS             complement(45715..47757)
                     /locus_tag="VF874_03150"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX9175116.1"
                     /translation="MDFYSAYRHGFVRVAACTIHTAIADPAANAESVLRMAQDCHDEA
                     VALAVFPELTLSGYSIEDILLQDALLDAVEEALVDVVTASTELVPVLVVGAPLRYRHR
                     IYNTAVVVHRGKVLGVVPKSYLPTYREFYERRQLAPGDNVRGVIRVDGAQVPFGPDLL
                     FAASDVPGFVLHVEVCEDMFVPVPPSAEAALAGATVLTNLSGSPITIARAEDRCLLAR
                     SASSRCLAAYVYAAAGEGESTTDLAWDGQTMIWENGRLLADTPRFPKGPQHCVADVDL
                     ELLRSERLRMGTFDDNRNHHADAAESFRRMEFQLDPPTGDVGLRRRIERFPFVPSNTE
                     RLEQDCYEGYNIQVAGLQQRLRALNYPKVVIGVSGGLDSTHALIVAARAMDRENRPRR
                     DILAFTLPGFATGERTKNNAIRLSRALGVTFEEIDIRPTAQLMLKEMGHPFGRGEKVY
                     DVTFENVQAGLRTDYLFRIANQRGGIVLGTGDLSELALGWSTYGVGDQMSHYNVNGGV
                     PKTLIQHLIRWVISSGQFESDVCEVLRSVLDTEITPELVPSGEEEELQSSEAKVGPYS
                     LQDFSLFQVLRYGFRPSKIAFLAWHAWSDPERGDWPPGFPPDKRPSYSLKEIRHWLQV
                     FVERFYSFSQFKRSALPNGPKVSHGGSLSPRGDWRAPSDMSARTWLDEIEREVPED"
     gene            47970..48842
                     /locus_tag="VF874_03155"
     CDS             47970..48842
                     /locus_tag="VF874_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HEX9175117.1"
                     /translation="MPETQSLRPHFEDVQAHYDLSDDFFQLFLDPTQTYSCAYFERDD
                     MTLEEAQIAKIDLALGKLGLQPGMTLLEVGCGWGSTMLRALEKCDVDVVGLTLSKNQK
                     AYVEQLLAVSDSPRSKRVLLEGWEQFHEPVDRIVAIGPLEHFGYDRYDDFFKLAYDLL
                     PADGKMLLHTIVQASKDELEQRNLPITMRHLRFFKFIMDEIFPGGRLPLVALVEEHAT
                     KAGFTVSRIQRLQPHYARTLDTWAAALEAHKDEAIAIQSEEVYDRYMKYLTGCAELFR
                     DGYTDVCQFTLEKG"
     gene            48847..48957
                     /locus_tag="VF874_03160"
                     /pseudo
     CDS             48847..48957
                     /locus_tag="VF874_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560926.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
     gene            49007..49831
                     /locus_tag="VF874_03165"
     CDS             49007..49831
                     /locus_tag="VF874_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HEX9175118.1"
                     /translation="MDAPELLAALAGRRIAVLTGAGMSTDSGIPDYRGPDSPPSNPMT
                     IRQFTSDPVFRQRYWARNHVGWRHMDRTAPNAGHRALAALERASVVSGVITQNVDLLH
                     TKAGSRTVVNLHGTYAQVICLGCGHTMTRAHLAGLLEARNPGFVADADGLAVAPDADA
                     VVADTSSFRVVDCPACGGMLKPDIVYFGENVPKHRVAQAFSLADNADALLVAGSSLTV
                     FSGYRFVRHAAARGIPVAIVNRGPTRGDDLATVKIDAGCSPILTLLADELVAGVMA"
     gene            49828..51660
                     /locus_tag="VF874_03170"
     CDS             49828..51660
                     /locus_tag="VF874_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9175119.1"
                     /translation="MTVEIRSPMAGTVIAVDVSTGQTVAAGAVLAVVEAMKMHHSLHA
                     EHGGRVEDIRVKPGDIVAAGDAVILLDPSDAGNTAAPEEQQIDLDEIRPDLAALFERR
                     AKLLDEDRPQAVARRHDRGMRTARENIADLTDDGLLVEYGGFAVAAQRGRRTISDLEA
                     ATPADGVITGLGRVNGEKFGPQRSTCAIVAYDYTVLSGTQGHFSHRKTDRILQQARRH
                     RYPVVLFAEGAGGRPGDTDAPNVAGLHYTTFAEMGALSGVVPMIGVVAGRCFAGNAAL
                     LGTCDVIIATADATIGMAGPVMIEGGGLGTFTPEQVGPIDVQTRNGVVDIAVADEAEA
                     VTAAKRYLAYFQGALPDYEVAEQRSLRHLVPENRQLAYDVRAVVRTLCDVDSVQELRR
                     AFGTCVVTALARIEGRSVGVIANDPAVLGGAIDADGADKLARFLQLCDAFGVRRFPLR
                     HTGIHGRPDAEKAATVRHFSRLFVLGGHLSVPMITVVLRKGYGLGAMAMAAGCFHNTS
                     ATVAWPTGEFGGMNLEGAVRIAARGTLSAIADADERQRTYDEMLAAAYRDGSAMNTAS
                     HLEIDDVIDPAETRAVIAATLLSAARPTGDGWANPNRRAGIDTW"
     gene            complement(51664..52146)
                     /locus_tag="VF874_03175"
     CDS             complement(51664..52146)
                     /locus_tag="VF874_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX9175120.1"
                     /translation="MHILVAFGSERHGTAGLATMIGDALRDLGHDVLVAPAKEVRRPE
                     GVDAVIVAGALYANRWHRDARRFVKRNRKLLQDLPVWLVSSGPLDDSAEQRDIPPTRQ
                     VQKLAALVGARGHVTFGGRLAPDAKGFPASAMAKTKAGDWRDAAHVQQWAKTVADELG
                     "
     gene            52253..53662
                     /locus_tag="VF874_03180"
     CDS             52253..53662
                     /locus_tag="VF874_03180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9175121.1"
                     /translation="MGHLSMLDASFLEAEDSDRHVSLAIGSVCVLEGPMPDDDALVSG
                     LAERVQTVPRFRQVLRTHLLDIEAPEWVEAPNFDISHHVHRVAVPHPGDDAALFRLTA
                     DVMERRLDRDLPLWECWIVEGLPDGQWAMIMKMHHCIADGIATMHMLTGMNDGGEGDT
                     YAADIRAAHEPPPRRLGLPQFSPDPRKWVSGAWERSTSLVGGATRALGGTIEILGGML
                     RPAPTSSLVGPVATMRRYAAAEVSLDDVKKVAHAYDMTLNDVALAAITDAYRAALIRR
                     GEKPQRNSLRTLVPVSVRSNDEVGKVDNRVSLMLPHLPVELSDPEDQLRAVHRRLMQA
                     KESGQRQAGAAFMSAANMIPFPLSAWTVRAMTRRPQRGVAALATNVPGPRHPTQIMGH
                     KVKRLFPIPPIALGLRTGIAIVSYADTLIFGITGDYDTAPDVDDIARGIERAVTRLVG
                     IASGSRHAVPLHANLRSGS"
     gene            complement(53609..54055)
                     /locus_tag="VF874_03185"
     CDS             complement(53609..54055)
                     /locus_tag="VF874_03185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9175122.1"
                     /translation="MIDSTSRADVANAERFARIGIPADAESASRIREEFAGWLQRFFG
                     LDPIRSSDLVLAINEALANAAEFAYVTTDRPGMMDLIAHYDPDSASLTATIADQGVWR
                     MPNPGPPARTRGRGIPLMKALSDRVTIETSTAGTQVRMQWDGVARP"
     gene            complement(54088..54456)
                     /locus_tag="VF874_03190"
     CDS             complement(54088..54456)
                     /locus_tag="VF874_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9175123.1"
                     /translation="MDEQHATKTTGCVVDEEWVGRVVVVSVSGVVDMLTAPQLEQAIR
                     AALDKKPGGLVVDFAEVEFLASAGMGVLVAVHDEVTPDMRFCVVADGPATSRPLKLVG
                     IADLIELYATRDEALSALSA"
     gene            complement(54571..56181)
                     /locus_tag="VF874_03195"
     CDS             complement(54571..56181)
                     /locus_tag="VF874_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9175124.1"
                     /translation="MTAVAVGANTTLTGTRGLLRLFVRRDRIVLPLWMLLLSVPLSTV
                     YVASIESVSPTAADRAQLAATIMSSSAQRALYGNVYNDSIGAVGLWKAGLFHLLIGVA
                     VILTVIRHTRAEEETGRAELLDSTAVGRYAGLTAALLLAFGASVATGLIGIACLLTTD
                     AEPSGSVAFGFALAGSGLVFSSVAAVAAQLSTNARTARGIAFAVLGAAFALRAVGDAS
                     SGTLSWLSPLGWSLQVRPYAGDRWPVLLLHLCTTAALTTVAYLLLRRRDLGAGLIADR
                     PGPATASPALSGPFGLAWRLHRGSLLAWTIGLCLYGGLVGNVVYGMGNQWGDNPTIRE
                     IVNRLGGSTAIENSFLAIAFSILAVTAAAQGVSAALRLHQEEAAQRGEAVLTTAVGRI
                     RWAASHLVPAITGSAVAILAAGVVAGLAYGVAAGAVATKLGTVMALAALQLPAIWMLP
                     AITVALFGLMPRFATVAWAVLIGFVALYLLGSIAGFPHWLLDLVPFTHTQRIPGEPFR
                     LAPVAWLLLIDAALIAVALAAFRRRDLR"
     gene            complement(56178..57065)
                     /locus_tag="VF874_03200"
     CDS             complement(56178..57065)
                     /locus_tag="VF874_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9175125.1"
                     /translation="MTAVIEVSDLHKHFGRVRALDGLDLTVNEGEIHGFLGPNGSGKT
                     TTIRVLLGVLRATSGHAGLLGRDPWRDAVALHREIAYVPGDVTLWPSLTGGEIIDLLA
                     RMRGGIDEGRRAELIERFDFDPKKKARTYSKGNRQKVSLISALSSRARLLLLDEPSSG
                     LDPLMERVFRECVIEARDRGVTVLLSSHILSEMEVLCEKVTIIRAGKTVESGTLESMR
                     HLSRTSIKAELAGPPPDLSRIPGVEDVTVEGNTVYAQVDAGSLGELIRVLGDTGVRTL
                     VSQPPTLEELFLRHYRVAA"
     gene            complement(57084..57338)
                     /locus_tag="VF874_03205"
     CDS             complement(57084..57338)
                     /locus_tag="VF874_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009472555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9175126.1"
                     /translation="MNANDIRTEVLSVLTGIAPEVDADEITDDELLRDQVDLDSMDWL
                     NFLIGIHKRLQVEIPESDYASLRTLGDVVRYVDARLTAVN"
     gene            complement(57345..58544)
                     /locus_tag="VF874_03210"
     CDS             complement(57345..58544)
                     /locus_tag="VF874_03210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9175127.1"
                     /translation="MIEFKMPALGADMDEGTLNEWRVKPGDKVTRGQIVAVVETTKAA
                     VEIECWHEGTVHELLVPVGETVQVGAALATLLEAGETASPPTPSVAPTVEAAEEAPAA
                     APEVVPAPRAAMAAPLPPHRRRWVSPAARRVATSLGVDIEAVTGTGPQGAVTITDVEH
                     AAAVKVPEPKPAVATDRATAMRRSIAAAMSRSKREIPHYYLADDVIMDTALTWLAERN
                     AQLSITERVLPAVLQLKAVALAAKRFKEFNGFYRDDGFEAVSDVHVGVAISLRAGGLV
                     APAVHNVDTKKLDELMSDLTDLVARARAFSLRSSEMSDPTITVTNLGDQGVDSVFGVI
                     YPPQVALVGFGKTAQRVSVIDKGIRVTTNVQATLAADHRASDGHRGALFLAAINELLQ
                     QPDQLEG"
     gene            complement(58541..>59228)
                     /locus_tag="VF874_03215"
     CDS             complement(58541..>59228)
                     /locus_tag="VF874_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9175128.1"
                     /translation="AALRHMSGGQFSVPLVVRMATGAGRQLAAQHSHSLECWYAHVPG
                     IKVVAPATVADAYGMLPSALADPDPVLIFEHVQLYNAAADVDTLTPTDISSAAVRRTG
                     SDVTVIAYGGCVPKALDAANELSLAGIDSEVIDLRVLRPLDTATIVESVRKTHRAVVV
                     DEAWRSGSLAAEISARIVENAFFDLDAPIARVCTAEVPIPYAKHLEQAALPQPDKIVA
                     AVQGLFGDRQ"
BASE COUNT         9974 a        20126 c        19571 g         9557 t
ORIGIN      
        1 tgcggcgtcg tcgacgagct cactgaccgc ggtgagttgt tgcaccactt gggctttgga
       61 caccctgtcg atcttggtga cgaccacgac gagcgtggtg ttcggagcga cggcccggat
      121 ctgctcgacg atccaccggt ctcccgggcc tatttcctcg tcggccggga tgcacaggca
      181 gatgacgtcg acctcgctgt aggtgttgcg caccaggtcg ttgagccgct tgccgagcag
      241 cgtgcggggc cggtgcagcc ccggggtgtc gaccagcacg atctggaagt tgtcgcggtg
      301 cacgatgccg cggatggtgt ggcgggtggt ctgcggacgg tccgacgtga tcgcgacctt
      361 cgcgcccacc agcgcgttcg tcagcgtcga cttgccggtg ttcggccggc cgacgaaaca
      421 gacgaaaccg gaccggaagt cagtcatgtt cgctggtcgc cgaacgtgaa cacagtgcga
      481 aaaatgggcc gaaaagtcgc agtggcttca cgttcggcgc ctcacacgac gttcccggcg
      541 cgatcggtga cgatgatcgc cgcgtcgggc gagagttcac gcactgccgc gacgccggcg
      601 tcgtcggcgt caccccccag cagcacggcc gcctccaatg cggtggcacc gctggacacc
      661 gccgcggcga ccgccgcctg cagcgctgtc agcttgagcg ccgacagatc aacgggtgcg
      721 gcggcatacg tgcggccgtc ggcatcacgg acggccgcac cagtggacgc gtccgcgcga
      781 gccatcgcgc ctctggccag caccaccagt ttcgcgtcct cgtcattcat tcctggccgt
      841 tccccttgcg cttgtccggg ctcacgagca ctgtgccgat ccgcacccgg ccgcgatggt
      901 catgcccacc ctcggcacgc agtcgcagcc cgtgcgacac cacctcggcc cccggcagcg
      961 gcacgcggcc cagttcgagt gccaacagcc cgccgaccgt gtcgacgtcg aggtcctcct
     1021 cgaattcgac accgtagagt tcgccgacgt cctcgatcgg cagacgggcg gacacccgga
     1081 aattcttgtc ccccaagtcc tctatcggcg cgacttcgtc ggtgtcgtat tcgtcggcga
     1141 tctcgccgac gatctcctcc agcacgtcct cgatcgtagc cagaccggcg atcgccccgt
     1201 actcgtcgac tagcagcgcc atgtggttgc ggttgcgctg catctcccgc agcagagcat
     1261 cgagcggctt ggagtcgggc acgaacaccg ccggacgcat cacctcggtc accttggtgt
     1321 cccggccgcc gttgcgggag taatacgtct gctggacaag gtctttgaga tagacgacgc
     1381 cgacgacgtc gtcgacgttc tcgccgatca ccggaattcg ggagtgccca ctgcgcacgg
     1441 ccagcgacgt ggcctgccct gcgttcttgt cgctttcgat ccacaccatc tccgtgcgcg
     1501 gcaccatgac ctcgcgggcc ggggtgtcgc cgagctcgaa taccgactgg atcatccggc
     1561 gctcgtcgtc ggcgaccaca ccgcgctgct gcgccaagtc cacaacctca cgcagctcga
     1621 tctcggacgc gaacggaccg ttgcgaaagc cacggccggg ggtcagcgcg ttgcccaaca
     1681 gcaccagcaa gcggctgatg ggggtcagca gcaccgaaat ggcttgcagc ggaacagcag
     1741 atgccagcgc gatggagtac gcgtgctggc gtccgagggt gcgcggggcc accccgatcg
     1801 cgacgaagct gatcaccacc atcagcgccg cggcgaccac cagaccccac ttcaggccga
     1861 ggtcatccca cagaaaggcg accaacagga ccgtcgcggc tgcctcgcat gtaatgcgca
     1921 gcagcacaat caggttgacg tagcgcgggc gctcggccat cacccttgcc aggcgaacgg
     1981 cacccggccg ctcgtcgcgc accagctctt cgacccgcgc catcgacacc gtactgagtg
     2041 cggcgtcgat ggccgcgaac aatccgccga gcgcgatcag cacgatcgcg cccaccaggg
     2101 caccgatgcc ggtcacggtt cgtcgaagta ccgcgacttg tccagcagcc gacggtcttt
     2161 ctcggtctgg cggtcctgct ggtacgcctt cacctgctcg gcgacccact cctcgagcag
     2221 ttggcgctgc agcgcgaaca tctccttctc ttccgcgggc tcgccatggt cgaacccgag
     2281 aagatgtagc acaccgtgaa tggtgagcag cgccagctcg tgtcccagcg aatgtcccgc
     2341 agcggcggca tgttccgcgg cgaactcagg gcacaacacg atgtcgccga gcaccgccgg
     2401 tccgggctcg ggcgcatcag gacggccgcc gggttcgagc tcgtccatcg ggaagctcat
     2461 cacgtcggtg ggacccggca ggtccatcca ccgcatgtgc agatcggcca tcgagttgag
     2521 gtccagcaga accatggaca gctcggcggc cgggtggacg ttcatcttgt cgatgacgaa
     2581 ccgggcgacg ctgatcagct cgtcctcgga gacgtcgagc ccggactcgt tggcgacctc
     2641 gatggacatc agcgtgtccg gtgggcgcgc cgttgtgcac ggttcatcga cagcgacgaa
     2701 tcctccgcgc gggcataggc gtcgacaatc tcagagacca gctggtggcg caccacgtcg
     2761 gcacttgtca actctgcgac gtggatgtcg tcgatgccgt cgaggatacc cactgctgca
     2821 cgcaatccgg atgtcgcgcc actgggcaag tccacctgcg taacatcccc tgtcacaacg
     2881 attttcgagc cgaagccgag ccgggtgagg aacatcttca tctgctcggg ggtggtgttc
     2941 tgcgcctcgt cgagaatgat gaacgcgtca ttaatagtgc gcccgcgcat gaaagccagc
     3001 ggcgcgacct caattacacc gccgctcatc agctttggga tcacctcggg atccatcatg
     3061 tcgtgcaggg cgtcgtacag cggacgcagg taagggtcga tcttctcgta cagggtgccg
     3121 ggcaggaacc caaggcgctc accggcttcc accgcgggcc gggtcaggat gatccgggtg
     3181 acctgcttgg tctgaagtgc gttgaccgcc ttggccattg cgagatacgt cttgcccgtg
     3241 ccggccgggc cgatggcgaa cacgatcgtg ttcgcgtcga tggcatcgac ataacgtttc
     3301 tggttgagcg tcttgggccg aattgtcttg ccgcgcctgg acaagatgtc aaggctgagc
     3361 acctcggccg gtgactcgtt ggcgtcgccg gtcagcatcg cgatgctgtg acgtaccacc
     3421 tcgggcgtga ggacttggcc gcccgccacc aacgcgatga gctccgtgac gacgcgctcg
     3481 gccagcgcga cgtcagcggg tgaaccggac agcgtgacgg tgttgccgcg agcatgcagc
     3541 tgcgcggaga gcacgcgttc cattgcgcga aggttttcgt ccgcggagcc aagcaggccc
     3601 acgatgaggt cgggcggaac atcgatgctg cttcgaacgg tcggtgcagc gcttgtctcg
     3661 cggggcgtca cgtggttacc agagcctgct ttctttcgta tggcgggctt tacaacgaat
     3721 gccagtctac cgccgggcgc tccggtgtcc cagcgaatac ggacctgagg gtcgcagtac
     3781 gtggcaatat tcgtggttgt cgcaaccgca cgcgtgcggg aggggaatac atgacgacgt
     3841 cgcgcttggt attcgatccg ttctcgcagg agttcttcga caatccgtac gagatatacc
     3901 ggcggatgcg cgatgaagag ccggtctact acgacgaaga gcgcgacttt tacgcgctga
     3961 cccgtcacga ggacgtggcg gcagccttca agaatttcga gacgttctcg tcggcctacg
     4021 gccttgatct ggccatggtg aaatccggag gaccgccact ggacatcaag ctcctccaac
     4081 tcatggatcc cccgcaacac cggcacatgg gaagcgtgtt gaacaagatg ttcacgccgc
     4141 gggccgtgca agcccaggaa tcgatgatca ccgcgacgat cgacaagtac ctcgacgcca
     4201 tcgacggcga tgagttcgac gccgtgcagc agctcaccgc gccgttcccg gtcgaggtga
     4261 tcacggcgat gctcggagtg cccgacgagt acgtccagca ggtgcgcctg tgggtagacg
     4321 cgacgctgca ccgcgagccc ggccagatcg agatgggtgc agccggtctg caggccgtcg
     4381 tcgaacagat ggcgttctat cacagcctga tacaggagca tcgggaccag ccccgcgacg
     4441 acctgttcaa catgctcatc gaggccgaga tcgaacgcga cgacggcgga aaatcacacc
     4501 tcagtgatat cgaaatcgcc ggtttcgcaa cgcaactcgg cggagcgggt gccgagacgg
     4561 tgaccaagct gctcggcaac gccgtctatc tgttcgcccg caaccccgac cagtggcaga
     4621 agttgctcga cgaccggagc aagatcccgg ccgcagtcga ggaacttctg cgcttcgagc
     4681 cgccggcaca gtacgacgtc cgctgctcca tcagagagat caccgtgcgc ggggtgacga
     4741 ttcctccgcg caaacccgtg ttccttctca tcggctcggc gaaccgcgat ccccgcgcgt
     4801 ggacggacgt cgacacgttc gacatcgacc gcaaccgcac cgaagcccaa aacctcggat
     4861 tgggctacgg catccacagt tgtctcggcg cagccctggc ccggatggaa agcgctatcg
     4921 cgctggagaa gctgccggac ttcatgccac gttacgaagt cgactgggat ggctgcaggc
     4981 gcgtgcacat gcaaaacgtg atcggctggt ccaacgtccc cgtacggatt attcgcgagc
     5041 ccaccgcgta gtcagcactc ccaacgcacc cagcgccacc gccgcggctg tcgacgtgcg
     5101 caggatcgtc gggcccagtc gcacggcaac cgcgccggcg gtcgtcagcg cctcgagctc
     5161 ctcatccggg attccaccct ccgggccgac cacaagaacg attgaatcag cctgcgccac
     5221 atgcttttcc gacagcacct cggccgccga ctcgtgcagg acgagtgtgg tgtcgtcggc
     5281 gatgcggggt agaagttcgg cggtcgacac cgggccgctg accaccggga tgtgcgcgcg
     5341 acgcgactgg cgagctgcgg accgggcgac cgcgcgccac cgccgcagtc ccttctccgc
     5401 acgcgcggaa tcccagcggg ccacgcatcg ggccgcctgc caggcgacga actcgtcagc
     5461 gccggcctcg gtcgccgatt cgatcgccag ctccgacctc tccgccttcg gcagcgcctg
     5521 caccaccgtg acggagggct gtgcgggcgg cacgctccac cggctcagca cacgcgcgtg
     5581 aagcgcgccg cggtcgacgt cggagacctc acatgacgcc agctctccgg cgccgtcgcc
     5641 gagcagcagc tgttcgccgg gccgaatccg ccgcacggtg gcggcatgaa accgttcgtc
     5701 acccgacacc gtcgcaaccg cgccctcggc gggcagcgcg tcgacgtaga agagcggccc
     5761 tcccaagtca gcgaccggtg aacgtctcac gcaggcgact gaagaatccg ccgccgccgc
     5821 ccgcggcgtg cggcgacttc acctccgcca cgtcgtcacg acgcttcttc agctcgcgca
     5881 gcagctcggc gtcgtggtga tccagccggg tcggcaccgt cacctcgacg tgaacgtgca
     5941 gatcaccgcg cactccggtg cgaagatgcg gcatcccgtg gccgcgcagc gtcaccaccg
     6001 acccgggctg cgtgcctccg ggaagtttga cctcggtggg gccatcgagg atcgcgtcga
     6061 cggtcacatc ggtgccgagc gcggcatcca ccatcggcac cgagacggtg cagtgcagat
     6121 cgtcgctgtc gcggatgaac acgtcgtgct gctgctcgtg gacctccacg tagagatctc
     6181 cggcaggacc gcctcccggg cccacctcac cctgtgcagc cagccggatc cgcatcccgt
     6241 cgccgacccc ggccgggatc ttgacgctga tctcgcggcg cgcccgtacc cgcccgtcgc
     6301 cgccgcaccg gtagcagggg tcgaggatga cctcgccgac accccggcag gtggggcacg
     6361 gccgtgacgt catcacctga ccgagcagcg agcgctgcac gctctgcact tcgcctcgtc
     6421 cgccgcacgt gtcgcagcgc accgggttgg agttgtcgtt ggtgcccttg ccttggcatt
     6481 tgtcgcacag cacggcggtg tcgacggtga cctgcttggt caccccggtc gcgcattcct
     6541 cgaggtccag ccgcaggcgc aacagcgagt ccgcgccggg ccgcacccgc ccgatcggcc
     6601 cgcgggatgc cgatccgccc ccgaagaacg cctcgaacac gtcgccgagg ccgccgaagc
     6661 cggtgccgaa tccatttccg ctggtcgcgg cgttttccat cgggtcgccg cccaagtcga
     6721 cgatgcgccg cttctccggg tcgctgagca cctcgtacgc gacgctgatt tccttgaacc
     6781 gcgcctgggc cccctcgtcg gggttgacgt cgggatgaag ctcgcgggcc agcttccggt
     6841 acgcgcgttt gatctccgcg tcactggcgt tcctgctcac tccgagcagc gcgtagtagt
     6901 cccgtgccac gagaaccttt ctacctacga cctaagacat cgccgatgta catggcaacc
     6961 gcagcgacac tagcgatagt tcccggatag tccattcgtg tggggccgag cacacccatg
     7021 ccgccgtaca cgtcgccgtc ggcgccgtag gtggtggtga ccaccgaggt ggcggccatc
     7081 tcctcgacct ctgtctcgtg gccgatccgc actgtcacct tgccggcctg ctgctgcgcc
     7141 tgaagcagcc gcaggacgac tacctgctct tcgagcgcct cgaggatcga ccgcagcgag
     7201 ccgccgaagt cggcggtgtt gcgggtcaga ttggcggtgc cgccgagcag cagccggtct
     7261 tcggcgtgct cgaccagcga ctccagcagc acggtggccg accggcccac cgcgtcgccc
     7321 aacgtgccct tggggccgat ctcggacgcc aggtcggcca cggccgccga cgccgccgac
     7381 aaccgcttgc cgtcgagcgc ctggcccagc atgtcgcgca gctgggcgag ctgatggtcg
     7441 tcgatgacgt cgccgagctc gacgacgcgc tggtcgacgc gacccgagtc ggtgatcacc
     7501 acgagcagca accgcgccgg cgtcagcgcc accacctcga cgtgccgcac ggtggacgac
     7561 gacagcgtcg gatactgcac gacggcgacc tggcgggtca gctgcgcgag cagccgcacc
     7621 gcgcggcgca gcacgtcgtc gagatccacg ccggattcca gaaatccctg gatcgcgcgc
     7681 ctctccgcgg tggacagcgg cttgacgtcg tcgagccggt cgacgaactc gcggtagccc
     7741 ttctcggtgg ggatgcgacc cgagctggtg tgaggctggg cgatgtagcc ctcggcctcc
     7801 aggacagcca tgtcgttgcg gacggttgcg ctggatacgc cgaggttgtg ccgctcgacc
     7861 aacgtcttcg acccgatcgg ctctttggtg gcgacgaagt cggcgacgat cgcgcgcaag
     7921 acgtcgaaac gacgatcttc agcatgtccc atcggattca cctcccactc gacccatttt
     7981 acggtgatca attgggtgga tccgcagcga gcacgccagc gttcggcgtt tgcgccaaag
     8041 acgactaccc ttctcgacca gtgatgagcc gcttgcgcga agaacagaat gcagtgatga
     8101 gccgcttgcg cgaagaacag aatgcagtga tgagcgatga tctttaaggg cgtccgcgac
     8161 ggcaagccgt atcccgatca cggtttgtcg tatcgcgatt gggctcagat tcccccgcga
     8221 cagatccgcc tcgacgagtt ggtcaccacc acaacggtgc tcgcgctcga ccggctgctc
     8281 tccgaggact cgacgttcta cggcgacctg ttcccacatg cggtgaagtg gaggggcgtg
     8341 gtctacctcg aggacggcct gcatcgcgcg gtgcgcgccg cactgcgaaa ccgggtggtg
     8401 ctgcacgcgc ggctgctgga catggacgag ctgcaggcgc agcgggccta gctcggccgg
     8461 ttgcgctgcc acggccacgt catcagcgcc cacacagcga ggatcagcgc ggccgcggtg
     8521 aagatgtaga ccggccacgg gccgagcaca tccaacatcg acgcggtggc tggtttgcgg
     8581 ttgaggaaac cgtaattcgt tccggcgatc ctgttgtacg tgaacgtgac ggccgcccag
     8641 atcgcggtgg tgacggccgc gacccgatag ctgcgccagg tgggccgcat gccccggccc
     8701 caggtcaggt agatcgcggc ccagacgacg agcaagtgaa tgctccagaa cgcgaggaaa
     8761 tgcaggctgg ggaagtccag gccgatgagg accggcgaca ccagcgcttg agcactcaga
     8821 accagccccc agtagtacgt gagcgcgaac gcccagtgcc gctgtgacca caacgcgtag
     8881 gccgcggcga cggtcgcgag gtcggtcagg cgcagcggta ccggtggcgg gatgcggggg
     8941 tcgaagaccg agtacatcag cgcggcgccg tacaccgcga aggtcagtgc ccccaggatg
     9001 cggccgagca gccgcgcttg ggattcggat tgccggcgtc ccgcccacac cagtgccacc
     9061 gctccgatca cgaacacggc cagcacggcc cagtgcgacg ggccgtacat ggagaactga
     9121 cgctcggcgg acatcgaagc taaccgagca gcgtccggac cacaccgtcg gccagcaggc
     9181 ggccgcggtc ggtgagcacc agcctgtcgg cgtcgcgcac caacaacccg tcagcgatcg
     9241 cggtctgtgc gcgcgccatc tccgcatcgt ccagcgcggc ggccggaagc ccgctgcgca
     9301 tccggatcga cagcatcacg gtttcggtgt gcgtctcgac gggcccgagc tcctcgaacc
     9361 ccgcgaccgg tagtgcccct tcggcaagcg cggccgcata tgcgttgggg tgcttgatat
     9421 tccaccaccg cgtcgatccg acgaacccgt gcgcgccggg gcctgcgccc caccactcgc
     9481 cgccgttcca gtagccgacg ttgtgccggc attcgccgcc gggtcgggcc cagttcgaca
     9541 cctcgtacca gtcgaagccg gcgtcggcca tctgtgcgtc gagcaattcg tagcggcgag
     9601 ccagcacgtc gtcgtcgggc gcgtcgacct cgccgcgacg cactcgccgt gccaacgcgg
     9661 tgccgtcctc gacgaccagc gcgtaggccg acacatggtc gacaccagcc tcgacggccg
     9721 tgtccaccga gcgcagcaga tcgtcgtcgc tctcccccgg cgtgccgtag atcaggtcca
     9781 ggctgacgtg ccgaaacccg gcggcaagcg cctcctcgac agcggccacc gcgcgtcccg
     9841 gtgagtggac gcggtccagc actctcaaca cgtgcggcgt cacggattgc atgcccagtg
     9901 acattcgcgt gtaccccgcg gccagcagcg cgtcgaacag ttccggggac gtcgactccg
     9961 ggttggcctc ggtcgtcact tccgcgtcgg gcaccaggcc gaagttctca cgaaccgcgt
    10021 caagcaccgc ggccaggcgg tcggcaccca gcacggacgg ggtgccgccg ccgacgaaca
    10081 cggtgtcgac gggttgggcg ccggccagtt cggccgccag ccgcagctcc gcgcgcagcg
    10141 cttctagcca ggcgtccggg ttcgcaccgc ccagttcggc gggcgtgtag gtgttgaagt
    10201 cgcagtatcc gcaccgggtg gcgcagaacg gcacatgcac gtagacgccg aactgacggt
    10261 cgggcgttct cgtcatgccc cgagtctgcc cggccccgcg ccgagactgc cgtgggcgag
    10321 gcacttactc ggacttttcc gcgcggaatg caaatttcgg cgcgagggac aaccactttt
    10381 gctcaccctg agcgcaaatc tgggccggtt agggcacacg cgggtgctca tggcacaatg
    10441 gccacgtgac cgtcgccagc ctccacctca acatctcgtt ggtggcgcgg cggcatgtcg
    10501 atttcaagcg cgtctgtagc tgttgctgtc tgccttgacg tcgtagaccc gcccacctga
    10561 acttccagct ggccgccgga tctgcgccgc cggacagcca ccggtggatt gcgcgttccg
    10621 aaggccggct ctgacgagga gccaccactc atgacacggc ccaccaccaa gcgcaaaggt
    10681 gaaggccaat gggcgctcgg ctatcgcgag ccgctcaacg ccaacgaaca gagcaagaaa
    10741 gacgacgccc cgctgaacgt gcgggcgcgc atcgagaaca tctacgcgct gggcggattc
    10801 gacagcatcg acaagagcga cctgcggggc cgtttccgct ggtggggcct ctacacccag
    10861 cgtgagcagg gctacgacgg cacctggacc ggcgacgagc acatcgagac catcgaggcg
    10921 aagtacttca tgatgcgggt gcgctgcgac ggcggcgcgt tgaccgtcga gcagctgcgc
    10981 acgatcggtg cgatctccac cgagttcgcg cgcgacaccg ccgacatctc cgaccgcgag
    11041 aacgtgcagt accactggat tcgcgtggag gacgtgcccg agatctggcg ccgactggag
    11101 gcagtcggcc tgcagaccac tgaggcgtgc ggcgactgcc cgcgcgtggt gctcggctcg
    11161 ccgctggccg gcgagtcgct cgacgaggtg ctcgacgctt cgccggcggt cgacgagatc
    11221 gtgcggcgct acatcggcaa gccggagtac gccaacctcc cgcgcaagta caagaccgcg
    11281 atttccggcc tgcaggacgt cgtccacgag gtcaacgaca tcgcgttcat cggcgtcaac
    11341 catcccgaac acgggcccgg cctggacgtg tgggtcggcg gcgggctgtc cacgaacccg
    11401 atgctcgggc agcgcctggg cgcgtgggtg ccgctggacg aggttcccga cgtgtgggag
    11461 gccatcacgt cggcgttccg cgactacggc taccggcggc tgcggtcgaa ggcgcggctg
    11521 aagttcttga tcaaggactg gggcgtcgaa aggttccggg aggtcgtcga gacggagtac
    11581 ctgaagcgtc cgctgatcga cgggccggcg cccgagccgg cggtacaccc gatcgaccac
    11641 gtcggggtac agcgactgaa gaacggacgc aacgcggtcg gggtggcccc gatcgccggg
    11701 cgggtatcgg gcacgatcct gtcgaaggtt gccgacatcg ccgagaacgc cggctccgac
    11761 cgggtccggt tcacgccgta ccagaagctg atcgtgctcg acgtgcccga cgacaagctc
    11821 gacgaggcgg tcagcagcct ggaggcgctc gggctgccgt cgcagccgtc acactggcgg
    11881 cgaaacctga tggcgtgcac cggcattgag tactgcaagc tgtcgttcgc cgagacgcgg
    11941 gtgcgcgcgc agggtctggt gccggagttg gagaagcggc tcgaggacat caacgcccag
    12001 ctcgacgttc ccgtcacgat caacatcaac ggctgcccga actcgtgcgc gcgcatccag
    12061 gtcgccgaca tcgggttcaa gggacagatg gtcgacgacg gcgatgggcc cgtcgaggga
    12121 ttccaagtgc acctgggcgg aagcctcggg ctggacagcg gcttcggccg caagctgcgt
    12181 cagcacaagg tgctcagcca cgacgttggc gactacatcg agcggatagt gcgcaacttc
    12241 gtgaaacaaa gaagcgaagg ggagcgtttc gcgacatggg ccctgcgagc ggacgaggcg
    12301 gacttgagat gagcgtgacg acggagaccg acctacggga actggccgag cggggcgccg
    12361 aggagctcga cggcgccgcc acgattgacc tcctgcgctg ggtcgacgag aacttccgtg
    12421 acgattacgt ggtcgcgtcc aacatgcagg acgcagtgct catcgacatg gccgtcaagg
    12481 tgcggcccgg cgtcgacgtg ctgtttttgg acacgggcta tcacttcgcc gagaccatcg
    12541 gcacccgcaa cgcggtcagc gacgtctacg acgtgaacgt gatcaacgtc accccggagc
    12601 attccgtccc cgagcaggat gcactgctag gcaagaacct gttcgcccgt gatcccaacg
    12661 aatgctgccg gttgcggaag gtggccccgc tgggcacggc gctgcgcggc tactcggcat
    12721 gggtcaccgg gctgcggcgg gtggaggcgc cgacgcgcgc gaacgcgccg gtgatcagct
    12781 tcgacgcggc gttcgggctg gtgagggtca acccgctcgc tgcgtggacc gaccaggaca
    12841 tccaggacta catcgacgcc tacgacgtgt tcgtgaatcc ccttgtattc gatggctatc
    12901 cgtcgatcgg gtgcgcaccg tgcaccgcga ggccagtcga cggagccgat ccccgcagtg
    12961 gccggtggca gggcataggc aaggtcgaat gcggactgca ttccgaatga gcccagcggt
    13021 cctcgttctc actgcgcacg gcagcgccga cccgcgcgcc gcggtgaaca cccacgccgt
    13081 cgccgaagag atccgccggc agcggccccg cctcgatgtg caggtggcgt tctgcgagca
    13141 atcgacgccg aacctgcgtg acgtggtggc cgagttgggc gacaagagcg tcgtcacgcc
    13201 gctgctgctc gccgacgcgt atcacgcacg cgtggacatt ccggcgatga tcgccgaatc
    13261 cggtgcagca caagccgatg tcctcggtga agacgaccgg ctggtgacgg tgctgcggca
    13321 acgcgtgcgc tcgttgggag tgtcacgcct ggattccgac gtcggcgtgg ccgtcgtcgc
    13381 ggtgggatcc tcacggcgcc aggcgaacac acgcacggcg gcggtcgcgc ccgcgttttc
    13441 ggccggcact cgctgggccg gggccgtcac ggcattcgcc accggtcggc gcccgtctct
    13501 cgacgaagcg acagagcagc tgcgcgcgag gggtgcgcga cggctggtga tcgcgccgtg
    13561 gttcctcgcg cacgggcgga tcaccgaccg tgtcgcggaa tatgctggtg cgcacggtat
    13621 tccgatggcc gagccactcg gcgcgcaccg cctggtcgcg gccacggtgt tggatcgctt
    13681 tgaccgcgca cgcgcggcaa cgaacccgct tgccgcttaa gaagtcggcg ccggctccag
    13741 ctcttcggtc tctggcaact gcgtgctgcc cgcgatcggc ggcactcgag tggcgcgcac
    13801 atacactgtg tcgccctcct tcaacgccag cgcctcggcg tcgccgcggg tgatctgcgc
    13861 ggtgaacggt gctccgttag ctgcggcggt gagctccacc ctcacctcga acccgaggta
    13921 gacgatccgg tctatcgtgg cgcgcatcac accgatgctc ttggcggtcc cgtcgctggt
    13981 ggccaccgcc atctccggat tgcgccccac ccgaatgtcg tgcggccgca ccaggattcc
    14041 gttcagtgcg gacacggtgc cgaggaacga catcacgaac ccgttcgcgg gctggtcgta
    14101 gacatcggtg ggtgagccga tctgttcgat acggcctttg ttcagcaccg cgatccggtc
    14161 cgagacgtcc aacgcctcgg attggtcgtg ggtcaccagc acggtggtga cgtgcacctc
    14221 gtcgtgcagc cggcgcagcc acgcccgcag gtcctcgcgc accttcgcgt ccagcgcgcc
    14281 gaacggctcg tcgagcagca gcacctgcgg gtccaccgcc agcgcgcggg ccaacgccat
    14341 gcgctggcgc tggccgccgg acagctgccc gggataccgg cgttgaaaac cgctgagccc
    14401 caccgtctcg agaaggccgt cgaccttgtc ctcgatctct gccttggggc gcttgcggat
    14461 cttcaggccg aatgcgacgt tgtcacgaac gttcatgtgc ttgaaggccg cgtagtgctg
    14521 gaacacgaag ccgataccgc gccgctgcgg cggcacccgc gtgacgtcgc ggccgttgat
    14581 cgtgatggtc ccggtgtcgg gctggtcgag gccggcgatc gcccgcaaca gcgtcgactt
    14641 tccggagccg ctgggcccga gcagggtcgt cagggacccc gccggcacag tgaaattgat
    14701 gtcctccagc gccgcgaatt cgccgtagtg cttgttcaca ccacgcaacg tgattgcgtc
    14761 ggtcatgtcc ctatctcctt gcccgaccta gacttccgcc cgcgcgcgcc gcgcgtccag
    14821 gacgacctgg acgatcagca ccagaaccgc gacggtcatc agcagcgtgg agatcgcata
    14881 cgcgccgtat tccgcgccgc ggttgtaacg gtccgatacc aacagtgtca gggtctgcga
    14941 cgttcccggc aagttcgacg acaccatgat gacggcgccg tactcgccga gcgtgcgcgc
    15001 gatcgtcagc accactccgt acgtcagccc ccatcggatg gacggcagcg tgatccgcca
    15061 gaacgtctgc caccacttgg agccgagggt cgcggcggcc tcctcctggt cggtaccgat
    15121 ttcgtgcagc accggctcga cctcgcggat gacgaacggc aacgtgacga agatgctggc
    15181 cagcacgatc ccgggcaggc cgaagatgat tttgaagccg agatcccgct cgacgaaccc
    15241 gaaggcaccg gccgatcccc acagcacgat cagggccact ccgacgacca ccggcgagac
    15301 cgcgaagggc aagtcgatga tcgcctgcaa cgcacccttg ccccgaaacc tgctgcgggc
    15361 caacaccaat gcggtcggga tcccgaacaa cacgttgagc ggtaccacga tcgcgacgac
    15421 cagcaacgac aactgcagag ccgaaatcgc ggcgggagtg gtgatctggg cgaagaattc
    15481 gtcaaggccc ggggagaacg aacgccacag gatcagccca accggcacaa ccaccagcac
    15541 caagacgtaa gccagcgcaa gggacctgct caggtacttc accagaggcg acggaatcac
    15601 ttcgccaact cctcccgctt ggccgcccgc gaccccagcg cccgcagaat caacagcaca
    15661 acgaacgaaa gagcaagcag gacaatcgag atcgctgcag caccggtgcg gtcgtcgttc
    15721 tcgatcaacg tccggatcca ctgcgacgac acctcggtct tgccgggcac cgcgccgccg
    15781 atcagcacca ccgagccgaa ctcgccgatc gcgcgggaga acgccagccc ggcgcccgac
    15841 agcagtgcgg gtgtcagcgc agggaagacg atggtccgga atattgtcgc gttgctggcg
    15901 cccagcgacg ccgcggcctg ttcagcctca tgatcgactt ccatcagcac gggttgcacg
    15961 gcccgcacga cgaacggcaa ggtcacgaac agcagtgcga caccgacacc gtactgggtg
    16021 tgttggagat ggatgccgac aggactgttc ggcccgtaca gcgccagtag caccacgctg
    16081 gccacgatcg tcggcagcgc gaacggcagg tcgatcaccg cgtccacgag ccgtttgccg
    16141 gggaagtcgt cgcgcaccaa cacccaggcg accagcagcc cgaagaacac gttgatgacg
    16201 gtgacggccg cggagatggt cagtgtcacc cggaacgaat cgagcgctga cggcgatgtc
    16261 accgcggtga cgaaagccga ccagccgccc ttcgccgact gccacacgat ggccgccagc
    16321 ggcagcagca cgatcaccga cagccacagc gtggcgacac cgatccgcag tgaggtcgtg
    16381 ccgcgaaacc ccaggccgcg ggtcgccgtg gcctggggtt tcgggtcaag cgcagctgtc
    16441 atccggtcgc ctgtttgtag atcttggtga tcaagccgtt gtccttgtcg aacaacgacg
    16501 aatccacggt gctccatccg ccgagctcgg cgacggtcca cagcttctgt gccggcgcgg
    16561 ggaaatcgtt cgcgaattgc tgcgccacac cggggttgac ggggcggaac ccggcctgcg
    16621 cccacaggcg ctgcgcctcc tcggtgtact ggaagttctt gaagtcggtg gcttcctgca
    16681 ggtgggtggt ggtgttgacg acggcgagcg ggttttcgat cttgaacgtc tgcggcggga
    16741 tgaggtgctg aacgggcttg ccctgccgct cgacgttgat cgcctcgttt tcgtagctga
    16801 tcagcacgtc gccgctgccc tgcaggaaga cgtcggtggc ttcacggccg gagccggggc
    16861 gaagcttgac gtgctggctc accagctgct gcacgaagtc cagaccagcc tgctgatcct
    16921 ggccgccgtt gctctttgcc gcatacggcg ccaacaggtt ccacttggcc gagcccgagc
    16981 tcagcgggct cggggtgatc acctcgacgt ccgggcgcag cagatcgtcc cagtcctgga
    17041 tgttcttcgg attgccctgc cgcaccacca gcgccaccac cgagccgaac gggatgccct
    17101 tcgtcacatc ggagttccag ttctcggaca ccttgccggc cttgacgagt cgggtgatgt
    17161 cgggctcgac ggagaagttc acgatgtcgg cgggcttgcc gtcgacgacg gaacgcgact
    17221 ggtcaccgga tgccccgtag gacgtggtga tcgcgatgtc cttgccctgc tcggtcgcat
    17281 tgaacgcggg gatgaccttg ctccagccag gctcgggcac cgcgtaggcg accaatgtca
    17341 gcgtcgcccg cgcattcgac gggccgttgc tgcccgcgac atcgctggcg ccgccgccgc
    17401 acgccgcgag cacggtggcc gtgagggcga gcgcggccgc gtgacgcagc cgtaggactt
    17461 tgggaaagcg gagcatggaa gacctttcac tgcaggggag ggaaggattg gctcacccgc
    17521 tgcggagagg cgacgaatca ggacgcaggc cgatcgatgg ccactaccga cgccaaaccg
    17581 caagcgggcg tgggttcatt tacaacagcg cacgtctgca acagcgcaat caccgacggc
    17641 gataagtgcc agcacatagc acttgttgtt gcctcgggcc gcttgcatgg cgggaagact
    17701 aacaggttca tgacgcacgc gcagattgat ggatcggctc actggctcac tgtgaaactg
    17761 tgatagcgtg aaactgtgaa gaacattacc gtgtccgtct ccgacgatgt ctatcgcaga
    17821 gcgaggatcc gcgcggccga gctcggtaag tctgtgagcg ccctggttac tgagttcttg
    17881 gactcgcttt ccggtgggaa tgcggaattc tctcgactag aaggcaagca gcggaaggcc
    17941 cagagtgaga tccaccgctt ccgcgcgcgc gatcgtttga gccgcgacga ggttcacgat
    18001 cgtgcggttc gttgacacca acgtcctgct ctacgcgatc tcgcgcgatc cagaggagcg
    18061 agacaaggcg cagcgcgcga acgagattct gaccgccagg gacctggctc tgtctgtcca
    18121 ggtcctgcag gagttctatg tccaggcgac cagaactagc cgaccggacg cgatcaccca
    18181 tgaccaggcc gtcgcgctgg tggagtcgtt catgcggttt cctgtcgcca caatcacggc
    18241 tgagctgatg ctggccgccg ttgcgacgca ttggcgcttc ggcgtgtcgt attgggacgc
    18301 cgcgatcctc gaagccagcc gctttctcgg ttgtgacgtc gtcctctccg aggacctcag
    18361 caacggcgaa gactacggcg gcgtacgggt ccagaatccg ttcagcggtt gagcagccac
    18421 acgacgatca ccagcaacac cagaattacc agaaccaggg tcacccgcga cttgggcaac
    18481 gcactcactc ccctggttcg ccgtgccgga tccgccgcag cagcccgatc ccccgcccca
    18541 gggcgccgtc gagcagcacc gccagaccgt aggccagcag cagcaccacc ggcatcacga
    18601 caatcgtctg cagcacgaag cccagtccgg aaagccacag ctcgacgccg tcccaccaat
    18661 tgaggaaacc caacatcggg tcagcgtagg cgccggtgtc gtggcgggcc agcgatgagc
    18721 cgcttgcgcg aagagcagag agcagcgtgc cagaattgcc gcgtgccggc cagtgcggac
    18781 tccacaacgt gtgtcatcgc cggcggcggg cccgccggca tggtgctggg gctgctgctc
    18841 gcccggggcg gggttccggt ggtggtgatg gaaaagcacg ccgacttcct gcgcgacttt
    18901 cgcggtgaca cggtgcatgc aagcacgctg cgcctgctcg acgagctcgg cctcggcccg
    18961 cggttcgcac aagtcccgca ccgcctgatc gagagcatcc gcatgcaggt tcaggatgtg
    19021 ccgatcagcc tcggcctcaa caggatcccg ggtgcacaca agcacatcgc gctggtgcca
    19081 caatgggatt tcctcgagct gctggccacc gcggccgagg aggaaccgac gttcacactg
    19141 atgcgcagca ccgaggtgca ttcggccttg gtgcgcgacg ggcgcgtggc aggtgtccgc
    19201 tatcgcggcg ctgacggtga gacgcgcgag atgcgcgcag cgttgaccgt ggcatgcgac
    19261 gggcgctcgt cgacgttgcg ctcgtcaatg gggttgcaac ccaaggcttt cggcgcgccg
    19321 atggacgtct ggtggttccg gttgccacgg cagagcggcg acccgacggg gctcaacggc
    19381 gtcttccgat ctgggcatgc ctgtgtcgta atcgaccgcg gtgactacta ccagatcgcc
    19441 tacatcatcc ccaagggcac ggacagcgcg atgcgcgccg agggcgtcgc ggccttgcgc
    19501 cgccaactcg tacagctggt gccgtggctc tccgatcgcg tcgagcagat cgcgtcgatg
    19561 gatgaggtca aacttcttga cgtgcaattg aatcgactgc atcgctggta caccgacggg
    19621 ttgctgttga tcggcgacgc ggcgcacgcg atgtctccgg tcggtggggt cggcatcaac
    19681 ctcgccgtcg ccgacgccgt cgccgccgcg gccgcgctcg ccgccccgct gcgcgaaggc
    19741 aatcttggaa agcgccagtt ggcccgggtg caggcacgcc gatggctgcc gacggtgctg
    19801 atccagtccg cccagcggct catccatgcg cgggtgatcg cagtcgcgat gagcgaggcc
    19861 gccgcggagt cgccgaaacc gccgctgccc gtgcggatga tgagcaagct gccggcactc
    19921 ggtgccgttg ccggctacgc gattgcgatc ggcccgctgc cagagcacgc gcccgcgttc
    19981 gcccgccgtg acgccccaat gtgacactcc gcctgttcag tagacgaaat ccgcgttcag
    20041 agccgatgat gagcaaatgg ttctgagcgc cgagcctgcc gaacagacac ccgaggcacc
    20101 gttttctgcc accagcccgg tgccgtacat ccagacgagc tcgacgcgca acccgttccc
    20161 gccgatcgcc gactacggtt ttttgtcgga ctgcgagaac acgtgcctga tcagctccag
    20221 cggctcggtc gaatggctgt gcgtgccgcg gccggattcc cccagcgtgt tcggcgcgat
    20281 cctggaccgg gcggcgggcc atttccggtt ggggccctat ggcgtgtcgg tgcctgccgc
    20341 gcgccgctac ctgccgggca gcctgatcat ggagaccacc tggcagacgc acaccggctg
    20401 ggtgatcgtg cgcgacgcgc tcgtgatggg tccctggcac gacctcgaaa cgcgctcgcg
    20461 gacgcaccgt cgcaccccga tggactggga cgccgagcac atcatgctgc gcacggtgcg
    20521 ctgcgtcagc ggcaccgtcg agatggtgct gaattgtgag ccgtcgttcg actaccaccg
    20581 catcagcgcc acctgggagt actcgggccc cgcgtacgga gaggcgatcg cgcgggcgaa
    20641 gcgggacccc gattcgcatc ctcagctgaa gctgaccacc aacctgcgga tcggcttgga
    20701 gggccgcgaa gcgcgggccc ggacacggct caaggaaggc gacaacgtct tcgtcgcgct
    20761 gagctggtcc aagcaccccg cgccgcagac ttacgaagag gccgccgaca agatgtggaa
    20821 gaccagcgaa tgctggcggc agtggatcaa catcggcgat ttcccggacc acccgtggcg
    20881 gtcgttcctt cagcgcagcg cgttgacgct caaggggttg acgtactcac ccaccggcgc
    20941 actgctggcc gcggccacca cgtcgctgcc ggaaacgcct cacggagaac gtaactggga
    21001 ctaccgctac gcgtgggtgc gcgactccac gttcgccctg tgggggcttt acaccttggg
    21061 cctcgaccgc gaggccgacg actttttctc gttcatcgcc gacgtctccg gtgccaacaa
    21121 cggggaacgc catccgctgc gggtgatgta cgcggtcggc ggggagcgca cgttggtcga
    21181 agaggagctg caccacctgt ccggctatga cggcgcccgg ccggtgcgga tcggcaatgg
    21241 tgcgtacaac caacgccagc atgacatttg gggcacgatg ctggattcgg tttatctgca
    21301 cgccaagtca cgcgagcaga ttcccgagtc actgtggccg gtgctgaaaa accaagtcga
    21361 agaggcgatc aagcactggc gcgagcctga ccgcggcatc tgggaggtcc gcggagagcc
    21421 gcagcacttc acgtcgtcga aggtgatgtg ctgggtggcg ctggatcgcg gcgccaagct
    21481 cgcagaactg cagggcgaga agagctatgc ccagcagtgg cgggcgatcg ccgaggagat
    21541 caaggccgac gtgctgaagc acggcgtcga ttcgcgcggg gtgctgaccc agcgctacgg
    21601 ctcggacgcg ctggacgcgt cgttgcttct ggtggtgctc acccgcttcc tgccgcccga
    21661 cgacccgcgg gtacgcaaca cagtgctcgc gatcgccgac gagctgaccc aggacgggct
    21721 ggtattgcgc tatcgggtcg aagaaaccga cgacggcctc tctggcgagg agggcacctt
    21781 cacgatctgt tcgttctggc tggtgtctgc gctggtggag atcggcgaag tcagccgcgc
    21841 gaagcatctg tgcgaacggc tgctgtcgtt cgccagcccg ctgcacctgt acgccgaaga
    21901 gatcgagccg cgcaccgggc ggcacctggg caacttcccg caggcgttca cccacctggc
    21961 attgatcaac gcagtggtgc acgtgatccg tgccgaagac gagggcgaca gcgccggggt
    22021 gttcgtcccg gcgaacgccc cgatgtagcg ctacttggcg ctggcgttct tgacgatttg
    22081 ctccacgacc tgccggccct cgtcgccgac gccgttagcg cacgcgactc cctcgacgat
    22141 gatgttggac actgtgccca ttgcgtgctg acagccccag ttgttgctgt cctgctggtc
    22201 catgtccata gacatcatcg tctcgctggg ctcttggagt cgcccgaagt cccagttgta
    22261 cgaggtgtca cttccctcgg tagtcgcgga gctctgctgg cacttcttcc attcgtcgcg
    22321 tgacttgtcg aggaagttgg ccgccttgtc tgcgttcgca aacagcacca cggtctgctc
    22381 cacccagtgc ttcttgttgt cgccgggttc gcggatgatc tggtcgcgca ccgccttcca
    22441 cccgctcttg tcgtagatct gctgttcagc ggaatagaag acacccagac actcggactt
    22501 gtcgatgctg tcggagttgt ccgcgagatc ttcagacgag ctactgagtt gcaggtcgga
    22561 gctgcctaca atcgagccga cgtcatcgat gctgagcagg atttggtcga gccccgactc
    22621 cgccaacggc ggcacgttag aacctgccgg ccccggcccc ttgactgctg taccaccgat
    22681 ggactgcgta cacccagaca gcacgatgcc agcagccgcg acgatcgcac agacccgcgg
    22741 cagcgccgca aagttgctca tgcatcattg ttacccgact cgggccgaca cggggcgcca
    22801 tcgtcatttc ttcggtttgt caccgccggc gtcagtggac agcgcggcga cgaacgcctc
    22861 ctgcggaacg tcgacgcgcc cgatcgtctt catccgcttc ttgccctctt tttgcttctc
    22921 gagcaacttg cgcttgcggg tgatatcgcc gccgtagcac ttgctcagca cgtccttgcg
    22981 caacgcgcga atgttctcgc gcgcaatgat tttcgagccg atggcggcct gcaccggcac
    23041 ctcgaactgc tgccgcggaa tcagctcctt gagcttgccg gtcatcttcc gaccgtactc
    23101 ggcggcggcg tcgcggtgca cgatcgcgct gaacgcgtcc accggctcgc cctgcagcag
    23161 gatgtcgacc ttcaccagct cggcctcctg ttcgccggcc tcctcgtaat cgaggctcgc
    23221 gtagccccgg gtgcgcgatt tcagcgcgtc gaagaagtcg aagatgatct cgcccagcgg
    23281 catcgtgtag cgcagctcga cccgctcagg tgacagatag tccatacccc ccagctcgcc
    23341 gcggcgggac tggcacagct ccatgatcgt gccgatgaac tcactcggcg agatgatcgt
    23401 ggtcttgacg accggctcgt agacggtgcg gatcttgccc gacggccagt ccgacgggtt
    23461 ggtcacgatc ttctcggaat cgtcctcaag aactacccga tacacgacgt tcggcgacgt
    23521 ggagatcagg tcgaggtcga actcgcgctc cagccgttcg cggctgatct ccatgtgcag
    23581 caggcccaaa aagccgcagc ggaaaccgaa tccgagcgcc accgacgttt ccggttcgta
    23641 ggtgagcgcg gcgtcgttga gctgcaaccg gtccaacgcg tcacgcagat cggggtagtc
    23701 ggagccgtcg accggataca gccccgaata caccatcggc ttgggctcgc ggtagccggt
    23761 cagggcgtcg gtggcgccgc ggcgcgcggt ggtcacggtg tcgccgacct tggattggcg
    23821 gacgtccttc accccggtga tcaggtagcc gacttcgccg acgccgaggc cgtcggccgg
    23881 tttcggctcg ggggagacaa tgccgacctc gagcagttcg tgggtggcac cggtggacat
    23941 catcttgatc cgctcgcgcg ggacgatctt gccgtcgatg acgcgcacat aggtgaccac
    24001 cccgcgatag gtgtcgtaga cggagtcgaa gatcatcgcc cgcgtcggcg cgtccgagtc
    24061 tccggtcggc ggcggcacct cacgcaccac gtggtcgagc agctcagcga cgccgtcacc
    24121 ggtcttgccc gagacacgca gcacgtcgcc gggctcgcag ccgatgatgt gggcgatctc
    24181 ggcggcatag cggtcggggt cggcggccgg caggtcgatc ttgttgagca ccgggatgat
    24241 gacgaggtcg cggtccagcg ccaggtagag gttagccagc gtctgcgcct cgatgccctg
    24301 ggccgcgtcg accagcagga ccgcgccctc acatgcctcc agcgcgcgcg acacctcgta
    24361 ggtgaagtcg acatgccccg gcgtgtcgat caggtgcaac acgtagtcct ggtcacccac
    24421 ggtccacggg agccggacgt tctgcgcctt gatcgtgatg ccgcgttccc gctcgatgtc
    24481 catccggtcc aggtactgcg cgcgcatcga acgttcgtca accacaccgg tgagctgcag
    24541 catccggtcg gccagcgtcg acttcccgtg gtcgatgtgg gcgatgatgg agaagttccg
    24601 aatctgcgcc ggcgcggtga acgtctggtc ggcgaagttg ctgatgggaa tctcctggtg
    24661 aggcggcaat gggctcagtt ccagggtatc gctgcctcgc tatgctcgat ttcatggcgt
    24721 cgccttggaa aacactccag cggcggttgg gcaaggccgc cgagaatttg gtgttcaacg
    24781 agggccccaa gttcatctcc cagggcatcc gtctcggact ggaggcgatc attcgcgggc
    24841 ccgagcccgc acgagagatc accgccggcc gtccggtgac caagaacagc gtgccgaccg
    24901 cgtaccgggc ccgcacgatc gtctactccc ccgatctcga cggccgcgcc gacccgggcg
    24961 agatcgtgtg gacgtgggtg gtttacgagg atgacccgtc gaagggcaag gaccgcccgg
    25021 tgctcgtcgt cggccgcgac cgcagcatgc tgctggggct gatgctgtcc agccaaggcc
    25081 accacgccga cgacccgaac tgggtcggca tcggggccgg gagctgggac tacgacggcc
    25141 gcgcgagctg ggtgcggctg gacagggtgc tcgacgtccc cgaggagggc atccgccggg
    25201 aaggcgcgat cctggggcat gagaagttcg agatcgtggc ggcccggctg cgcgccgaat
    25261 actcctggag ctagagctgc gccgtacgtg agctggctgc gaaaagatcg cgaaaatttc
    25321 gcagcgggcg cacgttcgcg gaggcggaac caggtccggc ttcgtcgcct ccagatatgc
    25381 cggcgcatgt tcggcgtcgc agcggtccag tcgcgaccgc atcagcgcct ggccgaggcc
    25441 cgcgccgcgg acggccgggt cggtgccgat gaccatcagg tctgggcggc cgttacgccg
    25501 aggacaccct gttgcacgag atggtccatg tcgcattgtt cgcacgcgat atcggcaact
    25561 tcgatgggca gaggcaccac aacacagacg tgtggtgcga ggagatcatc cggatcacgc
    25621 cgcaactggg cctgccgccg atcaaggccg ccccggtgaa gccgcgccgc atagacggca
    25681 aggtggcacg ccgtgcactc gagggccaca tatcgcgcga tgacatggcc cattggccgc
    25741 actcgctgcg gccggccggt ttctacaacc gcgagggccg catccacgtc ccgatctgat
    25801 ccttgagtgc ggtgctctat tttcggtcgc tcagatcgcc ttagacgcac caacagccac
    25861 ccccgcgggt gatccccggg tgttctgctg gtcaggaaat gtcggccgat ggcttcggtg
    25921 aatgagctgt gcggccagcg cacgtttgcc aagatgaact gaggcgcagt gtcaactttg
    25981 gccccagcgt gaggagtcat catgatgttc acccgccacg acatcaccaa gctcgtcggc
    26041 acagcgatag cggcagccgc cctgggtctg accaccgccg gcagcgccgc cgccgactcg
    26101 accgatgacg cattcctcca cagggtcgcc gccgacggcc tcatgttctc ccttcaggaa
    26161 gccgttatcc agagggccca cgtggtctgc gaggcgttct ccaccggcgc gtcgccggcg
    26221 agcgttcacg cgaccatgct caacaactcg gcaatgagtc cgcgccagac cgcgcttttc
    26281 atggccgacg cggtccaggc ctactgcccc aagtacgccg acctgctctt cagctaggtc
    26341 gagctggtcg ctgtagtcgc cgatcagcgc gcagggccga gcgtgccgtc agcattgccc
    26401 aatcacgcgc tacccggcgt cgggcgaccc accgtcgcga acatccgcgg cagcgctctg
    26461 gcgcgggtgg cgcgtcgggc agcatccacg acatcaccgg gtcgtcgtga aatgccacgg
    26521 ccagaacagc tgatgacctg cggacgtcgg ccttgttcgc gggccgtgcg ccgatggtca
    26581 tccgcgggtg atgtaggtct gcagctgctc ccgttcagca cccagttcat ccatgcgggt
    26641 cttgaccacg tcgccggtgc tgacgattcc ggccagcttg ccgtcttgga tcactggcac
    26701 gtagcgcacc cggttctcgg tcatcaaccc gctgaggagg tcgaccgcgt ccctaggtgt
    26761 acaggtcacc gccacaaccg tcatgatcgc cgacacgggc tgcgcgagaa ggtcggcgcc
    26821 gtgctcgttg agcttgcgca cgatgtcgcg ctccgacacg atgccgacca cggcgtcgga
    26881 gtcgacgacg accatggcac cgatgttctg ctgcgccagc ccggccagca gttcggtgac
    26941 cgttacgtgc ggctcgacag tggccaccgc tgagcccttg ttccgcagca catccgcgat
    27001 ccgcatcgcc gcctccgcct gttcgtgacc tggttgacac caggctatga ccaatcgtgg
    27061 cggcgcgaaa gcaaaagcct cgacttcgta acgcccgtat gacatacctg aggacatgat
    27121 cgaggtcatc ctgctcggca ccggtagtcc cattccggat ccgcagcgag cagggccctc
    27181 gaccctcgtc cgcgcgggaa gccagacgct gctcgtggac tgcggtcgcg gggtgttgca
    27241 acgggccgcg gcggtcggcg tcggcgcgaa cgcgctgagc gcactgctgc tcacccatct
    27301 gcacagcgac cacatcgccg acctcggcga cgtgctgatc acccgctggg tcacaacgtt
    27361 cacgcccaat ccgtcgccgc tgccgatcat tgggccgccg gggaccgcga gtgtcgtcga
    27421 cgcgatgctc gacgcgttcg gcccggacat cggataccgc atcgcccacc acgcggacct
    27481 gaccgcaccg cccgcggtcg aagtcgagga gaccacccag ggcgaggtgt gggcccgcga
    27541 tggggtgcga ataacggtgg cgcccaccga ccaccggccg gtggcgccga cgatcgcgtt
    27601 ccggatcgag tccggcggcg cgtcggtggt gctggcgggc gacaccgtgc cgtgcgacag
    27661 cctcgacgag ttggccgcgg gcgcgaatgc actggtgcac acggtgattc gaaaagactt
    27721 ggtggagtcg ttgccgcagc agcgggcccg cgacatctgc gattaccact cgtcggtcga
    27781 ggaggcggcg gcgaccgccg cgcgcgccgg ggtcgacacg ctggtgctga cacactgtgt
    27841 gcccgcggtc gctcccgggc aagaagacga gtggcgggca agggcggcat cggcgttcgg
    27901 cggccgcgtc gaactgggtc ccgacctaca ccgcgtcgaa gtgggcgctt aggccgcacc
    27961 gccggctgaa accatcgcac atcgggttac accgaaatct gcacgagggt cgcaatccgg
    28021 cgaaaaccac agccctggtg cagagctcgc ggcgaggcgc tgagccgcgc ctaggccagc
    28081 ctggtgattt cgaccaccac gtcgagatcg gttgaaccgc cgccggaata gatgcctttg
    28141 agggggctga cgtcggcgta gtcccgcccg aggccgacag tgacgtactg ctcgttgatc
    28201 tctttgtcgt tcgtcgggtc gaagttccac cagccgccgg tccacgcctg aatccaggcg
    28261 tggctctgcc cgtcgaccgt ctcgccgacg gtcgccttgc ggtgcggatg aaggtagccg
    28321 gagacgtacc gcgacggaat tcccatgctg cgcaacaaca ccagtgtcag gtgcgcgaag
    28381 tcctggcaga cgcctttgcc ttcgcgcagc gcgtcgaggg ctgacgaatg cacgccggtg
    28441 gttccggcga cgtaatcgag ttcgctttgc acccaccccg acgcggcgat caccgcgtcg
    28501 taagggtcgt agtccctgac gatccgttcg ccgacccggc caagccgtcg gctgtgcggg
    28561 gtgtaatggg tgggggtgag cacctcgtcg aaccggtcga ttaccgcctc ggtgttcagt
    28621 tcgtcccagg tcaccttctc cgacggcggc tcgggtttgt cagtctcgac gacggaggat
    28681 gcggtgacct ccaactcggt gtgcggcgcg tgcaggtcga acgcggtgac cgcggtgccc
    28741 cagtagtcga tgtagcggta cgaccttgtc gcgggcacgg tttcgacccg gctcaggatg
    28801 acgttctgcc ggttgtccga ccgcggggtc agccgcgcct cgttgaacga cgaggtggcc
    28861 ggcgacttgt acgcgtaacc ggtcgagtgc accacccgaa gccgccacat cagctgtctc
    28921 tcttcgcgca agcgctcatc gctagatctc cccttcctct atcaccagcg agccgttgcg
    28981 accggcgtcg ctccacgcca cccacggggc ggagtggaag tactgttgcg ccaacgctcc
    29041 tcccagttcg cggcatgtct gctggattgc acccaggcgt tcctccaatg actccagcaa
    29101 gactcccggc cgcaggaact ccaattcgct gcgggctcgg cccagcagcc gttgcgcttc
    29161 ggctgtcgcg ccgacgcggc tgtgcggtcg atggtcgagt tcgtcgaggc tgtgctcggc
    29221 cagccgcagc gagtagaaca ccgaccgcgg gaaaagccgg tccagcagca tgaattcgac
    29281 cacgcgggtg gcgtcgagca cgccccggta ggtccgcagg taggtgtcgt gcgcgccggc
    29341 cgaccgcagc agcgtcaccc acgccggcga cgacgcgctg tcccctaccc gggacagcaa
    29401 cagccgcaca gtcatgtcga cccgttcgat cgccctgccc agcaccatga accgatagcc
    29461 gtcgtcgcgg gacagtgtgg agtcggccaa ccccgcgaac attgcggcac gaccctcgat
    29521 gtaggagaaa aactcatgtg ggccaaggcg tttggcggcg cgttcacgtt cgggcagcgc
    29581 gttgtaggtg gtgttcaggc actcccacag ctccgtcgac gtcacttcgc gggcggaacg
    29641 tgcgttctca cgcgcggcag agatcgcgtc gacgatcgag cagccgtcct ccaggccgcg
    29701 ggtgaacgcg accagatcgg tcagcgacca cacgttgagc ggcgaattgg gcggggcgac
    29761 gcccaagacc cgcaacagca cacgtgacgt gtggtcgggg tcgacgctgg agtcctcgag
    29821 cagctggtgc acggtcacgt ccaggatgcg cgcggtgtcg tcggcgcgtt cgacgtagcg
    29881 accgatccag tacagcgcct cggcgttgcg ggccaacatc agttgttcac ctcccgctat
    29941 tgctgctgct gttgctgctg ctgtgacgac gtgagcccgg ggcccatttc ggtggcggca
    30001 ggcacacggg actctcgcgg cagcttgcgc accacttcgg cggccgcgag ctcgcggtcg
    30061 ggcgccgacg cccgcgacgc cagcacccag gtgtccttgg agccgccgcc ctgactggag
    30121 ttcaccacca gcgacccctc ggtcagcgcg acgcgggtca gcccgccggg cagcacccac
    30181 acgtcgtcgc catcgttcac cgcgaacggg cgcaggtcga catggcgggg ggcgagccgg
    30241 tcgccgatct gggtgggcac ggtagacagc tgcacgacgg gttgcgcgat ccatccgcgc
    30301 gggtcgttgc ggatcttctt cgcggtggcg gccaactccc gctcggaggc gtcgggcccg
    30361 aagacgatcc cgtagccacc tgacccctcg accggtttga tcaccagctc gtcgatgcgg
    30421 tcgagcacct cctcgcgttc gtcctcgagc caacaacgga atgtgtcgac attggccagc
    30481 agcggcttct cgcccaggta gtactcgatg atcgtcggga cgtaggtgta gatcagcttg
    30541 tcgtcgccga ctccgttgcc gaccgcgctg gagatcacca cattcccggc gcgggcggcg
    30601 ttgagcagcc cggccacccc gagcaccgag tccggccgga actgcatcgg atccaggaag
    30661 tcgtcgtcga tgcgccggta gacgacgtcg acttgacgtt cgccttccgt cgtgcgcatg
    30721 tacacctggt tgtcgcggca gaacaggtcg cggccctcga cgagttcgac gcccatctgg
    30781 cgggccagca gcgagtgctc gaaataggcg gagttgtaga cgccaggggt gagcacgacg
    30841 atcgtcggat cggcctcgtt ggtcgcggcg gaattgcgca gcgcgcgcaa caaatgcgac
    30901 gagtagtcgc ccacggtgcg gaccctgttg gtggcgaaca ggtttggaaa cacccgcgcc
    30961 attgtgcggc ggttctccat cacgtaggac accccggacg gagagcgcag gttgtcctcg
    31021 aggacgcgga acgtgccgtc ggcgtcgcga accaggtcga tgccggccac gtggatgcgc
    31081 acgccgttcg gcgggttgat gccggcggcc tcgcggtgga aatgcttgca cgacgtgacg
    31141 agccgccgcg ggatcaccgc gtcgcgaagg atgttctgct cgccgtaaat gtcgtcgaga
    31201 tacatctcca gcgccttgac ccgttgcgtg atgccgcgct ccagccgcga ccattcgggg
    31261 gcggagatca cgcggggaac gaggtcgagc ggaaacggcc gctcctggcc ggacagcgaa
    31321 aacgtgatgc cctggtcgat gaaagcgcgg ccgagcgcgt cggagcgggc ggccagctcg
    31381 gacgcgtcgg atggcgccag ctcggtgtaa atgcccttgt agggggcccg cacggtgtcg
    31441 aagttgtcga acatctcgtc gaacgccgac gcatagggcc cgcgtttgcg gtaaccgtcg
    31501 aagattccgt cgaacttctt cagcgggtgt gagttactgc gtgtcgactg tccattgctt
    31561 gtcaggctgc gaacgctcac ctcccacatg ctgcaccagt ctggccgttt tggcaggcca
    31621 gcaggccggc tggtagcctg aacagtcgct gcccgctcgg cggccgtcga ttcaacccag
    31681 tggagaaacc gaaggaactc acgcgtggcc aacatcaagt cgcaggagaa gcgcaatcgc
    31741 accaatgagc gccgcagact gcgcaacaag tcggtgaagt cgtcgcttca cacggcgatc
    31801 cgcgcgttcc gtgaggccgc tgagagcggt gacaaggaca aggccaccgt gttggcgagg
    31861 gcggccagcc gcaagctgga caaggccgcg agcaagggcg tcatccacaa gaatcaggcg
    31921 gcgaaccgca agtcgtccct cacgctgacg ctgaacaagc tctgatctag cggcgccgcc
    31981 ccactagttc cgcgatgcgt cgcgtcgtcg cttccagcac gtagtcggcg tcggcggctg
    32041 cgcccttcac gtccgcgttc agggtggcga cgagacgcat cgcctcggcc acggactgcg
    32101 tcgaccaccc gcgcgcctgt cgttgcgcct tcttcacccg ccacggcggc atccccagct
    32161 ctgacgccag gcggtacggg tcacccgata acgggccgac gcgggcgatg gtgtgaacgg
    32221 cctcggcgag cgcgtcggcc agcacgacgc gcggttcccc gcggatcatt gcccagcgca
    32281 gcgcctcgac cgcaccggcg acgtcgccga cgacggcctt gtcggcgatg tcgaagcctt
    32341 tgacctccgc cctgccgctg tagtagcggc gcaccgcact gacgtcgacg tcgccgccgg
    32401 tatcggcgat cagttgcgag catgcggcag ccagttcgcg caggtccgac ccgaccgcgt
    32461 cgatgatcac gctgaccacg tcgtcgtcga catcgaggcg ctgctcacgg aattccccgc
    32521 ggacgaactc ggcgcgttcg ccggccttcg tgatgcgggc acaggggtgt aggtgcgcgt
    32581 cgagcttgcg cagctcgtcg gcaagcgcct tggcgcgtcc gccaccggag tggacgacga
    32641 ccagcaccgt gccatgcggc agatccgcgg cggcagagga aatcaaggcc gcggcgtcct
    32701 tgcctgcttc tgcggcggcg tcgagcacca ccacgcgttc gtcggcgaac agcgacgggc
    32761 tgagcagttc ggcgagctcg ctggtggtca cttcgccggc gcgcaacctg ctctccggga
    32821 cgtcgtcggt gccggcctgg gcgcgggcgt cgcgcagcac cgcggcgatc gcgcgctcga
    32881 cgagcagttc ttcatcgccg agcaccaaat gcagtcccgg tctagagctc actcgacgat
    32941 cgtgccatgg acggccgacg ccgctctctg gcgcgcgcga cgagccgcca cagcagcacc
    33001 gtgccgagcg caccggcagc gaccagcacc atgccgggca gccgcgacgg cacctcgacg
    33061 gtcgcgccgg gaacgccgcc ggcccagcgc gcgatattga gtagccacca cagctccgga
    33121 cccgtgaacc ggatcagcag gtgcgctgca ccgggccaca gcggcgccag cgcggcggcg
    33181 gcggtgcccg cgacggtgat cggagggatc accgcggtga ccgcgacgtt ggccacgatc
    33241 gacaccacgc tgatgcggcc gctgatcgcg gcgatcaacg gggccgtcac cacctgagcc
    33301 gccgtggcga cacagacggc gtcggccacc ggtttgggcc agccgcgcgc gaccaggcgg
    33361 ccagaccaca tcggcgcgat cacgatcagg gccgcggttg ccgacaccga cagcgcgaac
    33421 ccgaggtcga ccgacatttc cggcgccacg atcatcagcg cgatcacggt cgcggaaaga
    33481 gcgggcatcg cgtgccggcg ccgatgcgtc agcacgccga gcaacccgat cgcgcccatc
    33541 accgcggcgc gcagcacgct ggccgacggc tgcaccacga tgacgaaccc gaccagtacc
    33601 agcgccgcca gcgcgaccgc cacccgcggc cccacgaacc gggccgtcag caggaccgcg
    33661 ccgcagacga tcgtcacgtt cgcgcccgag accgcggtca ggtgcgtcaa tcccgcggcc
    33721 cgaaacgccg cgacggtttc gggcggcagg gtcgacgtgt cgccgagcac cagcgcgggc
    33781 agcgccgcgg cctggtcggc gggcagctgg gagcgcacgg cgtcggcgaa gcggcggcgc
    33841 accgattccg cggcgcgctg cacgggcgac gcctcgccga aagtcggtgt gccgctggcg
    33901 gacagcacag cgacggacag gtcgcgccgc gtcggccgcc cgacgagcgc gcggaaactc
    33961 gcgggccgcc cgggtgccaa ctcgccgaag ccgcgggccg gtgcgaacac cgtgacccgg
    34021 ccggacgcgt cgtcgccgcc gatgcgcctc agcgcggcgc ggaacaccac tcgtccgcca
    34081 tcgcgcaccg gtcgcggctg ctcactggcg gtgaccgtga cgtccgtgga tctgctgtac
    34141 agcgcggcga gcggatggtg ctcgagggcg cttactcgca cggcccccgc ccagccgaac
    34201 ccggccccga tcaccgccgc gccgagaacc accgcggaca ccgccgctcg caccggtgcg
    34261 ccttggcggc gggcgcgcca gccggccacc gcggcactcg ctgcgaccag gacgcagacc
    34321 accgccgcgg ccgtgccggc ccgccacacg atagcgatgg cggtcactgc ccaacaggtc
    34381 aatgccgctg gtaccaaccg ggcgtccacg ctcgtcaccg gcgcgcctca tacccgcacc
    34441 agacttcgga gcttctccaa gcgggtcgga ccgatgccgt cgacgtctgc caactggtcg
    34501 acgctggtga acttgccgtt ggcatcgcgc caggccacga tcgcggccgc ggtcaccggg
    34561 ccgatgccgg gcagcgtgtc gagttgttcg acggtggcgc tgttcaggtc gatcgggccg
    34621 gccgccggtc cgctggaaga tgtggacgag cccggcgagc ccgggccgcc ggaggacggg
    34681 gtcacgccgc tgccgagttt ggtcggctca cccggcgcag gggctatccc gaccacgatc
    34741 tgttcgccgt cggccacccg ctgcgccatg ttcagcccga tcaggtcggc accgtcgagc
    34801 gtgccgcccg ccgaggtgac cgcgtcggcg atgcgagcgc ccggttgcag tgtcaccaga
    34861 ccgggtttgt gcaccaaccc gaccacgctg accaccaccg gcccgtcagc gatcgccgtc
    34921 gacggggtgg cgctcgggct cggcgcagct gacgaaacca tctgcaccgg aggcagtttc
    34981 gcggacacga cggcgggtgg tttgtcccgc atcatcgcga aggccgtgac aatcacggcg
    35041 atcaccccga cggccgccag cgcgaagagc ccggcgcgtc ccggatcggc cctgaccgcc
    35101 gccagccagg cgggaccagt gtcttgtgac gctgacggca gccacctgga cagcgacgtg
    35161 tcttggctgt cggactcgtc ggcggtgccg tcaggctcgg agttcaggcg gcgatgtagg
    35221 cgctcggcgg gcaactctgt tcgcataggc gcgaccgtaa aaggcgtcgg cgacaccgga
    35281 cccgcccgag gagacggctg cgcgccggct ggggatgaat tcgaaactgt ggataaccgg
    35341 cgctgccgtc gggcgatctc tggcgcgcga catactcgac agtcatgacc agccagcctg
    35401 ctcgtcctct tcgtgtcatc caatggacca ccggcaacat cggccagcgg tcgctgcacg
    35461 cgatcatcgg ccggcccgac atggagttgg tcggcgtgta cgcgcacgga gtcgagaagg
    35521 ccggaaagga tgcggcggac ttgtgcggct ggccggagcc cacgggtgtc acggcgacca
    35581 acgacatcga cgcgctgatc gcgcttagac ccgatgcctg ttgctataac ccgctgtggc
    35641 cgagcgtcga tgagctggtg cggctgctcg aggcaggggt caacgtgtgc tcgagtgcgg
    35701 cgtggatcac cggcggtaag caaacgccag aggaccggga gcgcatcgaa aaggcctgtg
    35761 tccaaggtaa ttcgacgatg ttcggcagcg gcgcccaccc cggcatgagc aatctggtcg
    35821 gcatggttct cagcggttcg tgcgagcgcg tcgacgagat ccgaatcacc gagtcggtgg
    35881 actgctcgac gtacgaatcc gcgggaaccc agaaggcgat gggcttctcg cagccccccg
    35941 acacacccgg cttggccgag agtgtgcgcc gcgaaagcga ggtgttcgcc gagtcggcgg
    36001 cgatgatggc cgacgcgatg ggcgccacgc tcgacaagat gaccttcgac gtgacgttca
    36061 ccgcggctac cgacgaatcg gacctcggct tcatgcagat tccgaaaggc accgtcgccg
    36121 gcgtgtacgg ctaccaccgc ggctgggtcg gcgaccgcaa cgtcgtcagc gtcgggttca
    36181 actggatcat gggctatcac gtcgacccac ccaagccgct cgagcacggc cacgtcgtcc
    36241 aggtattcgg gctgcccaac atgcgcgccg tgatccattg cctgccgccc aaggactgga
    36301 cggagcccgg cttcatgggg ctcggtatga tctacaccgc gatgccggtc accaatgcgg
    36361 ttccgaaagt cgttgccgcc cagcctggaa tcgtcacgct ggcggatctt cccccgatca
    36421 cgggtcgtcc gccgactccg tgatctcgac gcacacggcc accgcacccg cgccgacgtg
    36481 gacgccgagc accgggccca tgtccgccac cgtcggcggc tcggccgggt gcaggcgcgc
    36541 gatcagctcg tgcgcaacgt cattcgcggc gctgaggttg tccacgtgct gcaccgcaac
    36601 gatcgccgac cgctcaccca cctcggtgac cacctgctcg accaacgcgt cgagcgcttt
    36661 gctcgcggta cgcacccgtt gcgccaacac cagcctgccg tcggggtcga tgcgcagcac
    36721 gggcttgatc gccagcgcgg tactgagcca cgacgccgcc gtaccgatcc tgccgccgcg
    36781 gcgaaggttg tccaaccgct gcgcgacaac gtagccacgg actcggccaa ctgccgaaag
    36841 agcctgcgcc tcaacgctat ctaggtcgca tccctcggct gcggcccgcg cagccgccaa
    36901 caccacgaac ccggttgcca gtgccacgga tttcgagttc accacccgca ccgcgccact
    36961 gaactgccgg gccgcctgct cccccgatgt gacggtggac gacaacgccg ccgagacgtg
    37021 aacggccacc accccgtcgc cgctgctgtc ctccaacgcc tgacggtagg cggtgctcaa
    37081 ctcggctggg gtggcgccgg cggtggtggt tttcggacga gagtgaatgt cgaccgggat
    37141 ggcgtcgacg ccgtcgcgga agtcctcacc gtcgcacaga atgtgcagtg gcacttggcg
    37201 gatcccccac tgctcgcgat cgccgtcgca caaccgcgac gacgagtcgg tgacgaccac
    37261 cacggccata gtcgctagcc ctgccggttc tcttgcgtca caccagcttc ggtcaacgca
    37321 ttcaacatca gctccgccac ggcctgatgc gcctcgaagt tccagtggat gccatcggga
    37381 ttgccgcgcc ccgacatcac gtgctcgacc acagcggctt tcagatcgac cagcggaacg
    37441 ccgtgttggc cagcccattc ggtgatcgcg tccactgtgc cgttccgccc gtggtgggcg
    37501 cggccataag tgtctgcgat gtgtacagac ggcagtgacg cgacgatcgg gatgcctggc
    37561 cgattgaaat cgacggcatt acgggtcatt tccaggtact cgaccgacag gtgtggcggc
    37621 agcgcagagc gagcgatcgg tgacagcctc ggctgcagcc agcaataccc gtcgcgtacc
    37681 cagcgccgca accaggcggg gcgcacgtag cggatcgact cacgcagtgc cgtcggcagc
    37741 ggcgacggga gcgagtccat accgctggtc gcgaagatca ccgcgcccgc gcgaggcagc
    37801 gccgcccacg cccggggatc ctgggtcgcg gcccaccaca cgtcgcgaca cgtccatccg
    37861 atgcggccga tcagctcgac atcccagccg agctgggccg caacgatatt cggccagatg
    37921 cgcggatcgt cggcgggcag gccgccggtc ggtccgtagt aagccagcga gtcggcgaag
    37981 atcaacagca cctgtcggtg cgcggcccgc tcagagagca tcgtcggcga cctgggccga
    38041 tgcgttccac acatccagcc gccatcggat gtcctccagg gaggcttcgg cggcggagtg
    38101 cccggacagc tgcacccagc tggcgttgcc catgccgccg agcaccggcc agttgtcggc
    38161 gggtaatccg agaagcttgg cggtcaacgc ggcgatcagt ccgccgtgcg ccaccagaac
    38221 cagaggccgg tcgggttcgt cggcgcccca atcggattgg gactcaacga gctccgcgac
    38281 caccggcgag ctgcgccggg ccacgtcgat gcgactttcc ccgccgtgcg gcacccacgt
    38341 cgcgtcgttg cgccactcga cgcgggcgcc gggcgcggcg gcgtcgactt ccccgtgcgt
    38401 catgccctgc cagttgccaa gatgcgtttc ccgcaaccgc tcgtccacga ccaatggcga
    38461 ccccgtccgc tcgcccagca cgacggcggt ctcgtaggca cggcgcagat ccgacgacac
    38521 gatcagcagc gggtgacgct tgcccagcac ttcggccacc gcgaccgctt gcgcacgccc
    38581 cagatcggag agctcggtgt cgagctggcc ctgcatccgg ctcccggcgt tgaactccgt
    38641 ctggccgtgc cgcaccatca ccagctggcg caccgtcatg acgtcccgtc cccgtccaaa
    38701 tcgacaggga tctccgggca gtcccgccac aaccggtcca gcgcatagaa atcgcgttcg
    38761 tcctcgtgct ggatgtgcac gacgatctcg acgtagtcga gcagcgtcca gcggccttcg
    38821 cgggcgcctt cgcgccgcgc gggtttgtac ccggcgcggc gcatcttctc ttccacttcg
    38881 tcgacgatcg cgttcacctg gcgctcgttg gacgcggacg cgatgacgaa ggcgtccgtg
    38941 atcaccagct gctccgacac gtcgaggacg acgacattgt cggcgagctt ggccgctgcg
    39001 gcgcgagcgg cgatggtggc catctcggtg gcttcggcgg aggccgtcac gacttgtcct
    39061 ggctcgcgca tcggtagagg ccgcgcttgg cgacgtactg caccaccccg tcaggcacca
    39121 ggtaccagat cgggcggttc tcgccggccc gggcgcgaca gtcggtggac gagatcgcca
    39181 gcgccggcac ttcgagcaac gtcaacgcgt cggagggtag cgacttgaac gccgctgaga
    39241 cgtgcgcgcc gtccaagtca tagccggggc ggctgacgcc gacgaagcgg gccagcgaga
    39301 acaactcctc gacgtcctgc caggtcagga tcgatgccag tgcgtcggcg ccggtgatga
    39361 agtacaactc gctgtcggga tgcagctcgt gcagctcacg caatgtgtcc ctggtgtagg
    39421 tacgtccacc gcggtcgatg tcgacccggc tgaccgagaa ccgcgggttg cttgcggtcg
    39481 cgatcaccgt catcaggtag cggtcctcgg cgggggtgac gtggcgaccg tgcttctgcc
    39541 acggctcacc agtggggacg aacaccacct catcgaggtg gaagcggtcg gcgacttcgc
    39601 tggcggcgac caggtggccg ttgtggatgg gatcgaacgt cccacccatc acaccgagcc
    39661 gctttcctcc tgacgccacg tttgggcagc ttacttaacc ggtgaaggcc cgcgtctgcc
    39721 tccagtggac gtcgagatct gcaccatggc tggggtcact cgcaaatcgc agccctggtg
    39781 cagaaatcga cgcagcggtg ggtccatcgc tcttgtagcc ttcgggcatg cgggcgacca
    39841 ttgataaggc aggacgattg gttatcccca agcaacttcg cgatcatcca ggactgcgac
    39901 cgggcaaggt ggaggtcacc gccgacggcg caggcctgcg tgtcgagccc ctcattgacg
    39961 actccctcgg agagcgggac ggacggcttg taattcccgc gcatggggta gagatcaatg
    40021 accaggtcgt gagaatcctg cgcgatgcca cccagcgcta acacggctgt gctgcctgcg
    40081 agcgaccgac ctgatcggga ccagctgctc agaccggcag cagcccgtcg ataacctccg
    40141 ccagctgctt ggcgttgcgg cattcgtgca tcgtgatcac gtcctgatag cgaggcaccg
    40201 cggaatcgcc gctgccccac agatgccgcg gctccgggtt cagccagtgc gcgtgccggc
    40261 tttcggtgac catccggtgc agcacgtcca cctcgggatt gcggtagttg ttgcgcccgt
    40321 cgccgagcac cagcagcgag ctgcgcggtg acagcacggt cgggtagttc tcggtgaacg
    40381 acgtgaacgc gttgccgtag tcgctgtgcc cgtcgcgggt gtacaccccg gaatcgcggg
    40441 tgatccgctg caccgccatc gccaagtccg cttccgggcc gaacatgtgc gtcacctcgt
    40501 cggtggtgtc gatgaaagcg aacacccgca ctcgggaaaa ctgttggcgc agtgcatgca
    40561 ccagcagcag cgtgaagtgg ctgaagccgg ccaccgagcc cgacacatcg cacagcacca
    40621 ccagctccgg gcgcgccggc cgcggcttgc gcagcaccat gtcgatcggc acgccgccgg
    40681 tgttcatcga cttgcgcagc gtctttcgca gatcgatctc acccgcccga gaccggcggc
    40741 ggcgtgccgc cagccgggtg gccagggtgc gggccagcgg cgccaccaca cgtcgcatct
    40801 gccgcagctg ctcacccgac gcccgcagga actcgacgtt ctccgacagt tgcggaatgc
    40861 cgtacatggc cacgtggtca cggccgagct gctcggcggt gcgccgcttg gtctccgcct
    40921 ccaccatctt gcgcagctgc gaaatacgct ggctagcaat ggctttggcg atctgctcct
    40981 gcgtgggcgt gggctcttcg ccgtagggtg ccagcagtcc cgccagcagc cgcccctcca
    41041 gctgatccaa cgccatcgcc ttgagcgcct gatacgacga gtacgacggg ccgcggctgg
    41101 agctgtaccg cccgtatgcc tcgacgatct gcgcgaccag cgcctccatc cgctcgtcgt
    41161 gatcaccgag atcactgtcg tcgcccagca attcgacaag ttgagcgcgc agggcgtcga
    41221 ggtcgtcggg cgaacccgag ctgtcctcgt cctcgatcgg cacagtggca cgtgcaccga
    41281 gcgcggccgg gaaccacagg tcgaacatcg cgtcgtacgt gtcgcggtgg tcgggccgac
    41341 gcagcacggc gcaagcgata ccctcgcgca gcacctcccg atcaccaagg cccagcacgc
    41401 tcatcacccg cccggcgtcg accgtctccg acggacccac cgaaatgcct tgtgctcgta
    41461 gcgcttccac aaagccaacc agatgccctg gaatgccgtg cggggcaagc ggctggggcg
    41521 ggcggacgcg gcgggcggcc atcagttcag cctcagctcc ccggcggcgc gggtgacgtc
    41581 ggattgatgc ttgagcacca cgcccaacgt cgacgcgatc gtcgcgtcgt cgatggtgtc
    41641 cagcccgagc gccagaagcg ttcggcccca gtcgatggtc tcggccaccg acggcagctt
    41701 cttcagctgc atgccgcgca gcaccccgat gatgcgcacc agctcctcgg ccagatgttc
    41761 cggcaactcg ggtactcggg acagcaggat tcgccgctcg aggtcggggt cggggaagtc
    41821 gatgtgcagg aacaaacacc gccgcttcaa agcttcggaa agttcacgcg tcgcgttgga
    41881 ggtcagcacg accaacggcg cgcgctcggc gacgatggtg cccagttcgg gcaccgtgac
    41941 ggcgaagtcg gacagcactt cgagcagcag gccctcgatc tcgatgtccg ccttgtcggt
    42001 ttcgtcgatc agcagcacgg tcggctcggt gcgccggatc gcggtcagca gcggccggga
    42061 cagcaggaac tcctcggaga acacgtcggt cttggtgtgt tcccagtcgc ccgagccggc
    42121 ctggatccgc aggatctgct tcgcgtggtt ccactcgtac agcgcacgtg actcgtcgac
    42181 gccctcgtaa cactgcagcc gcaccagccc cgagccggtg gcctgcgcga ccgcccgggc
    42241 cagctccgtc ttgcccacgc ccgcaggccc ttcgaccagc agcggcttgc cgagccggtc
    42301 ggcgagaaaa accgctgtgg cggtggcggt gtcgggcaga tagcccgttt cggcgagtcg
    42361 gcgcgccacg tcgtcgatgt cggcgaacaa cggggcgggc cgtgcgggga cgctcacttc
    42421 atactcctgt tgtcaggccg ggcgggtatg gccatcgccc cacacgatcc acttggttga
    42481 cgtcagctcc ggcaggccca tcggcccgcg cgcgtgcagc ttctgagtcg agatgccgat
    42541 ttcggcgccg aaaccgaact gctcgccgtc ggtgaacgcg gtcgacgcgt tgaccatcac
    42601 cgccgcggcg tccacctctt cggtaaaccg ttgtgcggca gccagattgc tggtcacgat
    42661 ggcctcggtg tggccggtgc cgtactcgtt gatgtggttg atcgcggcat ccagcccgtc
    42721 taccaccgcg acggcgatgt ccatcgacag gtactcgcgg cgaaggtcgt cctccgcacc
    42781 gccggagtga atcgtcaccc cggccgactc caacgcgttg gtcagccgcg gcaacgcctc
    42841 gccggcgatc gcggcgtcca ccagcagcgt ctcggcggca ttgcagacgc tgggtcgacg
    42901 ggtcttggcg ttcaacagga ttcgttcggc gacgtcaagg tccgccgcct cgtggacgta
    42961 cacgtggcag ttgccgacgc cggtctcgat ggtcggcacc tgcgcatcgc gcaccaccgc
    43021 gtcgatcagc ccggcaccgc cgcgcggaat caccacgtcg accagcccgc gagcctggat
    43081 caagtgcgtg acggtggaac ggtcttcggc cgacagcagc tgtgccgcgt cgggtggcag
    43141 gttctcggtc gccagcgcag cccgtagcac gttcaccagc gcggtgttgg aatgcgacgc
    43201 cgacgaactg ccgcgcagca gcaccgcatt gccggacttc agcgtgaggc cgaatgcgtc
    43261 aacggtgacg ttcgggcggc tttcgtagac gatgccgacc acgccgaacg gcacccgctg
    43321 ctggcgcagc tgcagtccat tgggcagggt ccgcccgcgc agcacctcgc cgatcgggtc
    43381 gggcaggccc gcgacctgcc gcagaccggc cgcgatgccg tcgacacggt gcgggttgag
    43441 cgccaatcgg tcgagcagcg cttccgaagt gccagcggcc cgcgccgcgt ccagatcctc
    43501 ggcgttggcg gccaggatca cgtgcatatg agacagcagc gcatccgccg cggcgtgtag
    43561 cgcgcggtcc ttgtcggcgg tggacaacgt cgccagcaga cgagaagcga cgcgcgcgcg
    43621 tcgggccgcg tcgtggacgt gctcacgcag gtcggggcgc gatggtgcct gcagactcat
    43681 caatccaggg tatcgggcca accgcccggt tgggcgcccg gggctaacct gagcgagtga
    43741 cgcgggtgtg cgtggtcggc agcgtgaaca ccgacctcac cttcaccgtc gacgcgctgc
    43801 cacgggccgg cgaaacggtg ctgtcctccg cgctggcgtc gtccccgggc ggcaagggcg
    43861 ccaaccaggc gctggccgcg gcccgcgccg gggcacgggt gcagttcgtc ggcgcggtcg
    43921 gcaccgacgc ggccgccgac gcgctgctgg cgcacctccg cagcaacgac gtcggcaccg
    43981 acgcagtgat caccgtgccg gggcccagcg gcacggccgc ggtcctggtc gacgccgacg
    44041 cggagaacat gatcgtcgtc gctgccggcg ccaacgctca tctcgtgctc gactcggcgt
    44101 tcatgcgcga cgtcgtcgcg caatccgacg tggtgctgct gcaactggag attccggcgc
    44161 cgacggctgt ggcggccgca cgcgttgccc gcaccgcgaa cgcgaccgtg atcgtgaacg
    44221 cgtcacctcc cagtacatcg ccggaaatgc ccgggttggc cgcactcgcc gacgtggtcg
    44281 tcgtcaacga atcggaagcc ggcgcatggc ggtgggccgt gccgcacctc gtgatcaccc
    44341 agggtgcacg cggtgcgacc tacatcgcgg acagccacga ggtcgacgtc gccgcgccgc
    44401 cggtcgagcc cgtcgacacg acaggcgcgg gcgacgtgtt cgcgggcgtg ctggccgcgg
    44461 gctggccagg cgatcctgac cgtgccctga ggcgggcatg cgcggccggt gcattggcca
    44521 ccttggtgcc cggtgcgggc gactgcgcgc ccaccaacga ggcaatcgag gatgcactgc
    44581 gaggctgctg atgtcattgt cagagacgcg acgccgcacc cttggccggc gaatccgcct
    44641 gctggtcgca gccacgatct cctacaacgt cgtggaagcg atgatcgcgg ttgcagaggg
    44701 aatccgggtc tcgtcatcgg ccctggtggg tttcgggctc gactctgttg ttgaagtgtc
    44761 atccgcggcg gccgtggcat ggcagttcag cgccaaagac cccgagtccc gggaaaaacc
    44821 ggccctgcgg ttcatcgcgt ggtcgttctt cgcactggcc gcctacgtca ccgtcgacgc
    44881 ggtgctgtcg ctgatcggca tcggcgaacc tcgtccgtcg gcgattggca ttgggttggc
    44941 ggctgtcagc ctgatcgtca tgccgctgtt gtcgttcgcc caacgatcag ccggccgcga
    45001 actcggatcg cgttccgccg tagcggattc caagcaaacg ctgctttgca cgttcttgtc
    45061 agccgcgctg cttctcggcc tggttgccaa cgccgtagcg ggatggtggt gggcggatcc
    45121 cgttgcggcc ctcctgatcg cggccgtcgc cgttcgcgaa ggccgcgaag cgtggcgtgg
    45181 tgatccgtgc tgcacggccc acgtcgagga actgcaatga acgacagggc aatggcgctg
    45241 atcgcgatcg cgacgtacgt cctcggcgcc atggtcggca tcgtctggcg ctcgtggtgg
    45301 cagtggcgcc gcaccgggtc gacgggcttc gagggcgtca gcgggcgcgt cggatcagcc
    45361 gagtggttcg gcggtatcgg tgtggtcctc ggagtggtgc tgacgttcgc cgcgcctgtc
    45421 ctgcaactgg ccgggatcgt tgagccgctg ccgttcttgc gcagggcatg gatccaatgg
    45481 ggcggcgtcg tcatcgccct cgcgggcatt acgttcaccg tgtatgcaca actcgccatg
    45541 ggcacgtcgt ggcggatcgg tgtcgacgac agcgagacga cgacgttggt gcgtagcggc
    45601 gtgttcgggc tcgtccgcaa tccgatcttc gtaggcacgt gggcgttctg gctcggcaca
    45661 acgctggtca ccccgaggcg ggcctgtgaa aatacggttt cggcgaaggg gagatcagtc
    45721 ctccgggact tcccgctcga tctcgtcgag ccaggttcgc gccgacatgt ccgacggcgc
    45781 gcgccaatcc ccacgcggcg acagcgatcc gccgtgcgac accttcggcc cgttgggcaa
    45841 cgccgaccgc ttgaactggc tgaacgagta gaaccgctcc acgaacacct gcagccagtg
    45901 ccgaatctcc ttcagcgagt aggacggccg cttgtccgga gggaagccgg gcggccaatc
    45961 gccacgctcc gggtcgctcc acgcgtgcca cgccaggaac gcgatcttcg acggccggaa
    46021 cccgtaccgc agcacctgga agagcgaaaa gtcctgcagc gaatacggtc ccactttggc
    46081 ctcgctgctc tgcaactcct cttcctcgcc cgagggcacc agctcggggg tgatctcggt
    46141 gtcgagcacc gaccgcagca cctcgcacac gtcggactcg aactgccctg aggagatcac
    46201 ccagcgaatc aggtgctgta tcaacgtctt cggaactccg ccgttgacgt tgtagtgcga
    46261 catttggtca ccgacgccgt acgtcgacca ccccagcgcc agctcggaca ggtcgccggt
    46321 gccgagcacg attccgccgc gctgattggc gatacggaac agatagtcgg tgcgcaagcc
    46381 ggcctgaacg ttttcgaacg tgacgtcgta aaccttttcg ccgcggccga acgggtgccc
    46441 catctccttg agcatcagct gcgccgtcgg ccggatgtcg atctcctcga acgtcacacc
    46501 caatgcacgc gacagccgaa tcgcgttgtt cttcgtccgt tcaccggtgg caaagccggg
    46561 cagtgtgaac gccagaatgt ctctgcgcgg ccggttttcc cggtccatcg cacgcgcggc
    46621 gacgatcagc gcgtgggtgg agtcgagccc gcccgacacg ccgatgacca ccttcgggta
    46681 gttcagcgca cgcagccgct gctggagccc ggccacctgg atgttgtagc cctcgtagca
    46741 atcctgttcc agccgttcgg tattcgacgg cacgaacggg aaccgctcga tgcgccggcg
    46801 caacccgacg tcacccgtcg gcgggtcgag ctgaaactcc atgcggcgga acgactccgc
    46861 ggcatccgcg tggtggttgc ggttgtcgtc gaacgttccc atccgcaacc gctcgctgcg
    46921 cagaagttcc aggtccacgt cggcgacgca gtgctgcggg cccttcggga accgcggggt
    46981 gtcggcgagc agccggccgt tctcccagat catcgtctgc ccgtcccacg ccaggtcggt
    47041 ggtcgactcg ccctcccccg ccgcggcgta gacataggcg gcaaggcacc gagaggacgc
    47101 cgagcgcgcc agcagacagc ggtcctccgc ccgggcgatc gtgatcgggc tgccggacag
    47161 gttcgtcagc accgtcgccc ccgccagcgc cgcctccgca ctcggcggca ccgggacgaa
    47221 catgtcctcg cacacctcga cgtgcagcac gaaaccaggc acgtcggacg cggcgaacaa
    47281 caggtcgggt ccgaacggca cctgggcccc gtccacccgg atcacgcccc ggacgttgtc
    47341 gccaggagcc aactgccgcc gctcgtagaa ctcccgatac gtgggcaggt acgacttggg
    47401 cacgacgccg agcaccttgc cccggtgcac gacgaccgcg gtgttgtaga tgcggtgccg
    47461 gtaccgcaac ggcgcgccga ccaccagcac cggcaccagc tcggtggagg cggtgacgac
    47521 gtcgaccagc gcttcctcga ccgcgtcgag cagagcgtcc tgcagcagga tgtcctcgat
    47581 tgagtacccc gacagcgtca gctcggggaa caccgccaac gcgacggcct cgtcgtggca
    47641 gtcctgcgcc atccgcagca ccgactccgc attcgcggcc ggatcggcga tcgcggtgtg
    47701 aatcgtgcag gcggcgacgc gaacgaagcc gtgccggtac gccgagtaga agtccatgac
    47761 gccattgttg cccccatctc tgctcggatg accaagtacg gggtatgcag atgccatgag
    47821 ccacaccgct gtgcgcctat cggcgttacg ggtttggcca aggcgtgtag cctgctctaa
    47881 ctagtggtga ggattatttg ccgggcgtcc acgcgggcat cgcagccgcc aaggactccc
    47941 cgcacccccc gttaggacag gactgaaagt tgccagaaac acagagcttg cggccacact
    48001 tcgaggacgt gcaggcccac tatgacctgt ccgacgactt cttccagttg ttcttggacc
    48061 cgacgcagac ctacagctgc gcttacttcg agcgcgacga catgacgctg gaagaggccc
    48121 agatcgccaa gatcgacctg gcgctgggca aactcggcct gcagccgggc atgacgctgc
    48181 tcgaagtcgg ctgcggttgg gggtcgacga tgctgcgcgc gttggagaag tgcgacgtcg
    48241 acgtcgtcgg cctgacgctg agcaagaacc agaaggccta cgtcgagcag ctgttggcgg
    48301 tgtcggatag cccgcgttcc aagcgggtgc tgctcgaggg ctgggagcag ttccacgagc
    48361 cggtggaccg gatcgtggcg atcggcccgc tcgagcattt cggctacgac cgctacgacg
    48421 atttcttcaa gctggcttac gacctgttgc cggccgatgg caagatgctg ctgcacacga
    48481 tcgttcaggc gagtaaggat gagctcgaac agcgcaatct gccgatcacg atgcggcacc
    48541 tgcgtttctt caaattcatc atggacgaga tcttccccgg cggccggctg ccgttggtgg
    48601 cactggtcga ggagcacgcc acgaaggccg ggttcacggt gagccggatc cagcggctgc
    48661 agccgcacta cgcgaggacg ctcgacacgt gggcggcggc gctggaggct cataaggacg
    48721 aggcgatcgc gatccaatcg gaagaggtct acgaccggta catgaagtac ctgaccggct
    48781 gcgccgagtt gttccgcgac ggctacaccg acgtgtgcca gttcacgctg gaaaagggat
    48841 agtcaggaca tcgtctgttc cgcgctcgac ggcgagcgac ccgacctggt gcggccgatt
    48901 gagcccgatc cggaatgccg cttcctcacc aaggtgcgcc acagtgcgtt ctactccacc
    48961 gcgctggatg cctatgtgcg gcatttctag cgcttaccct cgaaccgtgg atgccccgga
    49021 actgctcgcg gcgttggcgg gccgccggat cgctgtgctg accggcgcgg gcatgtcgac
    49081 cgactccggc atcccggatt accgtgggcc cgattcacca ccgagcaacc cgatgacgat
    49141 ccggcagttc acctccgacc cggtgttccg gcagcggtac tgggcgcgca accacgtcgg
    49201 ctggcggcat atggatcgga ccgcgccgaa tgccggccat cgggcgctcg ccgctttgga
    49261 gcgggccagc gtcgtgtcgg gggtgatcac gcagaacgtc gatctgctgc acaccaaggc
    49321 gggcagccgc accgtggtca acctgcacgg cacctacgcg caggtgatct gcctgggctg
    49381 cgggcacacg atgacgcgcg cgcacctggc cggactcctc gaggcgcgga acccgggttt
    49441 cgtcgccgac gcggacggac tcgccgtggc gcccgacgcg gacgcggtgg tggccgacac
    49501 ctcgtcgttt cgagtcgtcg actgcccggc atgcgggggg atgttgaagc ccgacatcgt
    49561 ctatttcggc gagaatgtgc ctaaacaccg tgttgcgcaa gctttttcgc tggctgacaa
    49621 cgccgatgcg ctgctggtag ccgggtcctc gctgacggtg ttctcgggtt accggttcgt
    49681 gcggcacgcc gccgcgcgcg gcatccccgt cgcgatcgtc aaccgcggcc caacccgcgg
    49741 cgatgacctc gccacggtca agatcgacgc cggctgttcg ccgatcctga cgctgctggc
    49801 ggacgaactc gtcgcaggtg tcatggcgtg acggtcgaga tccgctcgcc gatggcgggc
    49861 acggtgatcg cagtcgacgt ctctacggga cagaccgtgg ccgccggtgc ggtgctcgcc
    49921 gtcgtcgaag caatgaagat gcatcattcg cttcacgcag aacacggcgg acgcgtcgag
    49981 gacatccgcg tcaaaccggg cgacatcgtg gcagcaggcg acgccgtgat cctgctcgat
    50041 ccctccgacg ccggcaacac agccgcgccc gaagagcagc agatcgacct cgacgagatc
    50101 cgtcccgatc tggccgcgct tttcgagcgg cgtgccaagt tgctcgacga ggaccggccg
    50161 caggccgtcg cccgtcgtca cgaccgtgga atgcgcaccg cgcgagagaa cattgccgac
    50221 ctcaccgacg acggcctgct ggtcgagtac ggcggcttcg ccgtcgccgc gcaacgaggc
    50281 cggcgcacga tctccgattt ggaagcagcc acccccgccg atggcgtcat cacgggcctc
    50341 ggccgggtca acggcgaaaa gttcggaccg caacgatcga cgtgcgccat cgtggcctac
    50401 gactacaccg tcctgtcggg cacccaaggc cacttcagcc accgcaagac cgacaggatc
    50461 ctgcaacagg cccggcggca ccgctatccc gtggtgctgt tcgccgaggg cgcgggcgga
    50521 cgccccggcg acaccgacgc gccgaacgtg gcggggctgc actacaccac gttcgccgag
    50581 atgggcgcgc tgtccggtgt cgttccgatg atcggggtgg tcgccgggcg ctgctttgcg
    50641 ggcaacgccg cgctgctcgg cacgtgcgac gtcatcatcg ccaccgccga cgcaaccatc
    50701 ggcatggccg gcccggtgat gatcgaggga ggcggactgg gcaccttcac tcccgaacag
    50761 gtgggcccga tcgacgtgca gacccgcaac ggggtcgtcg acatcgcggt ggccgacgag
    50821 gcggaagcgg tcaccgccgc gaagcggtac ctggcctact tccagggcgc gctgcccgac
    50881 tacgaggtgg ccgagcagcg ttcgctccgc cacctggtgc ccgagaaccg ccaactcgcc
    50941 tacgacgtgc gcgcggtcgt gcgcacgctg tgcgacgtgg attcggtgca ggagttgcgg
    51001 cgcgcgttcg gcacgtgcgt cgtcaccgcg ctggcccgca tcgagggccg atccgtgggc
    51061 gtgatcgcca acgatcccgc cgtgctcggt ggcgcgatcg atgccgacgg cgccgacaag
    51121 ctcgcccggt tcctgcagct gtgcgacgcg ttcggcgttc gtcgtttccc tttgcgacac
    51181 accgggattc atggtcggcc cgacgcggag aaggcggcaa cggtgcgtca cttctcgcgg
    51241 ctgttcgtgc tcggcggcca tttgagcgtg ccgatgatca cggtcgtgct gcgcaaggga
    51301 tacggcctcg gcgcgatggc gatggcggcc ggatgcttcc acaacacctc cgcgaccgtg
    51361 gcgtggccga ccggcgagtt cggcgggatg aacctcgaag gcgcagtgcg catcgcggcc
    51421 cgaggcacac tcagtgccat agcggatgcc gacgagcggc agaggactta tgacgagatg
    51481 ctggccgccg catatcgcga cggcagcgca atgaacacgg catcgcatct ggagatcgac
    51541 gacgtgatcg accctgccga gacacgcgcc gtcatcgcgg cgacgctgct gtcagcggcg
    51601 cggccgaccg gtgacggctg ggccaacccg aaccgccgcg ccggaatcga cacgtggtaa
    51661 gcgtcagccg agctcgtcgg ccaccgtctt cgcccattgc tgcacatgtg ccgcgtcgcg
    51721 ccagtcgccg gccttggtct tggccatagc gctggccggg aaacccttcg cgtcgggcgc
    51781 gagccgtccg ccaaaggtca catgcccgcg cgctccgaca agagcagcga gcttctgcac
    51841 ctgacgcgtc ggcggaatgt cgcgctgctc ggcagagtcg tccagcggac ccgaactgac
    51901 cagccacacc ggcaggtcct gcaacagttt acggtttcgc ttgacgaagc ggcgggcgtc
    51961 gcggtgccag cggttggcgt acagcgcgcc ggccacgatc accgcgtcaa cgccctcggg
    52021 gcggcggacc tccttggcgg gcgccacgag cacatcgtgc ccgaggtcgc gcagcgcgtc
    52081 cccgatcatc gtcgccaatc cggcggtgcc atgccgttct gaaccgaacg caacaagaat
    52141 gtgcatgctc cgatcctcgt gggacaggcg ttcccgctgt agaggcacat tgcccggtgt
    52201 gagaggacca tcgccccttc cgaagtgcga ccgcagggcg catcgtagtg tcatggggca
    52261 tttgtcgatg cttgacgcga gctttctcga agccgaagac tccgaccgcc acgtcagtct
    52321 cgcgatcggt tcggtgtgcg tgctcgaagg cccgatgcca gacgacgacg cgctcgtatc
    52381 cgggctcgcg gagagagtgc agacggttcc gcggttcaga caggtgctgc gcacgcatct
    52441 gctggacatc gaagcgccgg aatgggtcga ggcgcccaac ttcgacatct cccaccacgt
    52501 gcaccgcgtc gcggttcccc accccggcga tgacgcggcg cttttccggc tcaccgccga
    52561 cgtgatggag cgacggctcg accgggatct cccgctgtgg gagtgctgga tcgtcgaggg
    52621 cctgccggac ggccagtggg ccatgatcat gaagatgcac cactgcatcg ccgacggcat
    52681 cgcgacgatg cacatgctca ccggaatgaa cgacggcggc gagggcgaca cgtacgccgc
    52741 cgacatccga gccgcgcacg agccgccacc gcgccgactg gggttgcccc agttcagccc
    52801 cgacccccgc aagtgggtca gcggcgcgtg ggagaggtcg acgtcgctgg tgggtggcgc
    52861 cacccgcgcg ttgggcggga ccatcgaaat cctcggcggc atgctgcgtc ctgccccgac
    52921 ctcgtcgctc gtgggtccgg tagccaccat gcggcgctac gccgcggccg aggtgtccct
    52981 cgacgacgtg aaaaaggtcg cccacgcata cgacatgacg ctcaacgacg tcgcgctggc
    53041 cgcgatcacc gatgcatacc gcgctgcact gattcgccgc ggcgagaagc cacaacgcaa
    53101 ttcgctgcgc acgctggtac cggtgtcggt gcgctccaac gacgaagtcg gcaaggtcga
    53161 caaccgggta tcgctgatgc tgccgcacct tcccgtcgaa ttgtcggacc ctgaagacca
    53221 actccgcgct gtgcacagac gattgatgca agccaaagaa agcggacagc ggcaggcggg
    53281 agccgcgttc atgtcagcgg caaacatgat tccgttcccg ctgagcgcgt ggacggtgcg
    53341 ggcgatgacg cgacgtcccc agcggggggt tgccgcactg gcgacgaacg tgcccggtcc
    53401 acgccatccg acgcagatca tgggccacaa ggtgaagcgg ctcttcccga tcccgccgat
    53461 cgcgctcggg ctgcggacgg gaatcgcgat cgtgagctac gccgacacgc tgatcttcgg
    53521 catcaccggc gattacgaca ccgccccgga cgtcgacgac atcgcccgcg gcatcgagcg
    53581 cgcagtgaca cgtctcgtcg ggatcgcgtc agggtctcgc cacgccgtcc cactgcatgc
    53641 gaacttgcgt tccggcagtt gatgtttcga tcgtgacgcg gtccgacagc gctttcatca
    53701 gcggaattcc ccgtccgcga gtacgggcgg ggggcccggg attgggcatg cgccagacgc
    53761 cttggtcggc aatcgtcgcc gtcaaactcg cggaatccgg gtcatagtgt gcaatcagat
    53821 ccatcattcc cgggcgatcc gtggtcacat acgcgaattc cgccgcgttg gccagtgctt
    53881 cattgatcgc cagcaccaga tcactggatc ggatcgggtc taggccgaag aaccgctgaa
    53941 gccatccggc gaattcttcc cggatgcgcg aggcactttc ggcatcggcc gggatgccga
    54001 tgcgcgcgaa acgttcggca ttggcgacgt cggcccgaga cgttgaatcg atcatggtgg
    54061 ttgatgacta cccggcgaag cctctttcta cgcgctcagc gcggacagtg cctcgtcacg
    54121 agttgcatac agttcgatca gatcggcaat accgacgagc ttgagcgggc gactggttgc
    54181 cggcccatcc gcgaccacgc agaaccgcat gtcgggcgtc acctcgtcgt gcaccgcgac
    54241 cagaacaccc attcccgccg acgccaagaa ttcgacttct gcgaaatcga ccacaaggcc
    54301 gcccggcttc ttgtcgagcg cagcccggat cgcctgctcc agctgcggcg cagtgagcat
    54361 gtccacgacg ccagaaacgg acacgaccac gacccggccg acccattctt cgtcgacgac
    54421 gcagccggtg gtctttgttg catgttgttc gtccacagtc acctccggtg tggattccgc
    54481 tcgcgaggct gcggtctgaa cctgcaaggt cagtcaatgt cgttagcgcc gatgacgacg
    54541 caactggaaa cagagtaacc cagggtcctg ctatcgaaga tcccgacggc gaaacgccgc
    54601 cagagcgacg gcgatcagcg ccgcgtcgat cagcaacagc catgccacgg gggccagccg
    54661 gaacggctcg ccgggtattc gctgggtgtg ggtgaacggg accaggtcga gcagccagtg
    54721 cgggaagccg gcaatggacc ccagcaggta cagcgcgacg aaaccgatga gcaccgccca
    54781 tgcgaccgtg gcgaaccgtg gcatcagccc gaacagcgcc acagtgatcg caggcagcat
    54841 ccagatcgcc ggcagctgga gggccgcgag cgccatcacc gtgcccagtt tggtcgccac
    54901 ggcaccggcg gcgaccccat acgccaggcc cgcgacgacg ccggcggcca ggatcgcgac
    54961 ggctgacccc gtgatcgcgg gtacgaggtg actcgcggcc cacctgatcc gtccgacggc
    55021 cgtggtgagt accgcctcgc cgcgttgcgc ggcctcctcc tggtgcaacc gcagtgcggc
    55081 cgacacgccc tgcgccgcgg cggtgaccgc gagaatgctg aaagcgattg ccaggaaaga
    55141 attttcgatt gccgtcgatc cacccagccg attgacgatc tcgcgaatcg tcgggttgtc
    55201 gccccactgg ttgcccatgc cgtacacgac gttgccaacc aggccgccgt agaggcacag
    55261 cccgatcgtc caggccagca gcgacccgcg atgtagccgc cacgcaaggc cgaacggacc
    55321 tgacagcgcc ggagacgcgg ttgccgggcc gggccggtcg gcgatcagtc cggcgccgag
    55381 atcgcggcgg cgcagcagca ggtacgcgac ggtggtcagc gctgccgtgg tgcacaggtg
    55441 cagaagcagc accggccacc ggtcaccggc atacggacgg acctgcaatg accaacccag
    55501 cggcgagagc cacgacaatg ttccggagct ggcatcgccg accgcacgca gcgcgaacgc
    55561 ggcgccgagg accgcgaacg cgatgccgcg tgcagtgcgc gcgttggtgc tgagttgggc
    55621 ggccaccgcg gcgacagaag agaacaccag ccccgacccg gccagcgcga acccgaacgc
    55681 gaccgagccg gacggctcgg cgtcggtggt aagcagacac gcgatgccga tgagtccggt
    55741 ggccaccgac gcgccgaacg ccaggagcag cgccgcagtg aggcccgcgt agcggccgac
    55801 agccgtcgag tccagcagtt cggcgcgacc cgtctcctcc tcggccctgg tgtggcggat
    55861 gaccgtcagg atcacagcca cgccgatcag caggtggaac aaacccgcct tccagagccc
    55921 caccgcaccg atgctgtcgt tgtagacgtt tccgtacagc gcgcgctggg cgctgctcga
    55981 catgatcgtg gccgcgagtt gcgcccggtc ggcagcggtc ggagagacgc tttcgatgct
    56041 cgcgacatag acggtggaca gcggcacgga tagcagcaac atccacagcg gcaacacgat
    56101 tcggtcccgc cggacgaaca gccgcagcag tccccgggtc ccggtcaatg tggtgttcgc
    56161 gccgaccgcg acggcggtca cgccgccacc cggtagtgac gcaggaacag ctcttcgagc
    56221 gtgggcggct ggctgaccag cgtgcgcacg ccggtatcac ccagtacgcg aatgagttcc
    56281 ccgaggctgc cggcatcgac ctgcgcgtag acggtgttgc cttcgaccgt gacgtcctcg
    56341 acgccaggga tccggctgag atcgggtggc gggccggcca gttcggcctt gatcgacgtg
    56401 cggctgaggt gccgcatcga ttccagtgtg ccgctctcga cggtcttgcc cgcccggatg
    56461 attgtcacct tctcgcacag cacctccatc tccgagagga tgtggctgga gagcaagacg
    56521 gtgacgccac ggtcacgggc ctcgatcacg cactcacgaa acacacgctc catcaacggg
    56581 tccagcccgg agctcggctc gtcaagcagg agcaatcgcg ctcgcgagga caaggccgag
    56641 atcagcgaga ccttctggcg gttgcctttc gaatacgtgc gcgccttctt cttggggtcg
    56701 aagtcgaagc gctcgatcag ttcggcgcgg cgcccttcgt cgatgccgcc gcgcatccgg
    56761 gcgagcaggt cgatgatctc gccgccggtc agcgacggcc acaacgtgac atcgccgggg
    56821 acgtaggcga tctcgcgatg cagagccacc gcgtcgcgcc acgggtcgcg acccaagagg
    56881 cccgcgtggc cggacgtggc gcgcagcacg ccgagcagca cccggatggt ggtcgtcttt
    56941 ccggagccgt tgggcccgag gaagccgtga atttcgcctt cgttgaccgt caggtccaac
    57001 ccgtcgaggg cgcgcacgcg gccgaaatgt ttatgcagat ccgaaacttc gataacagct
    57061 gtcattcccc cgcctggtcg tcctcaattc actgcggtca gccgggcgtc gacgtagcgc
    57121 acgacgtcgc ccagcgttcg caaagaagcg tagtccgact cggggatctc gacctgcagc
    57181 ctcttgtgaa tcccgatgag gaagttcagc cagtccatgg aatccaggtc cacctggtca
    57241 cgcagcaact cgtcgtcggt gatctcgtcg gcgtccactt cgggggcgat gcccgtcaag
    57301 accgacaaca cctcggtgcg aatatcatta gcgttcactg gctttcaccc ctcgagttgg
    57361 tcgggctgct ggagcagctc gtttatcgcc gcgaggaaca gcgcgccgcg gtgtccgtcg
    57421 ctcgcccggt gatccgctgc gagcgtggcc tgcacattgg tggtgacccg aattcctttg
    57481 tcgatcaccg agacccgctg cgcggtcttg ccgaaaccga ccagcgcgac ctgcggcggg
    57541 tagatcactc cgaacacgct gtcaacgccc tgatcgccga gattggtgac ggtgatggtg
    57601 gggtccgaca tctcggagct gcgcagtgag aaagccctgg cccgtgccac caggtcggtg
    57661 aggtcgctca tcaactcgtc gagcttcttg gtgtcgacgt tgtggaccgc cggtgccacc
    57721 aagccgccgg cgcgtagcga gatggccacg ccgacgtgga cgtccgagac agcctcgaac
    57781 ccgtcgtccc gatagaatcc gttgaactcc ttgaagcgct tcgctgccag cgcgaccgcc
    57841 ttcagttgca gcacagccgg cagcacacgc tcggtgatcg acagctgggc gttgcgttcg
    57901 gccaaccacg tcagcgcggt gtccatgatc acgtcgtcgg ccaggtagta gtgcggaatc
    57961 tcgcgcttgg accggctcat cgcggccgcg atcgagcgcc gcatcgctgt cgcgcggtcg
    58021 gtggccaccg ccggtttggg ctcgggcacc ttcaccgcgg cggcgtgctc gacgtcggtg
    58081 atggtcaccg cgccctgcgg gccggtgccg gtgacggctt cgatgtcgac gccgagcgat
    58141 gtggcgacac ggcgcgctgc cggcgatacc cagcgccgtc ggtgcggcgg aagcggcgcc
    58201 gccatcgccg cccgcggtgc cggcacgacc tcaggagcgg cggccggcgc ctcctcggcc
    58261 gcctccacgg tcggcgcgac cgatggcgtt gggggcgagg cagtttcgcc cgcctccagc
    58321 aacgtggcaa gagccgctcc cacttggacg gtttccccga ccggcacgag cagttcgtgc
    58381 accgtgccct cgtgccagca ttcgatttcg actgcggcct tcgtggtctc gaccaccgcc
    58441 acgatctggc cacgggtcac cttgtcaccg ggtttgacgc gccactcgtt cagcgtgccc
    58501 tcgtccatgt ccgcgccgag ggctggcatc ttgaattcga tcattggcgg tcaccgaaca
    58561 ggccctgtac cgccgcgacg attttgtccg gttgcggcag cgccgcctgt tccaggtgct
    58621 tcgcgtacgg aatcggcacc tctgcggtgc agacgcgggc gatcggggcg tcgaggtcga
    58681 agaacgcgtt ctcgacgatg cgggcgctga tttcggcggc caggctgccg ctgcgccagg
    58741 cctcatcgac caccacggcg cggtgagtct tgcggacgga ctcgacgata gtggcggtgt
    58801 cgagcggccg gagcacacgt agatcgatga cctcggagtc gattccggcc agcgacaact
    58861 cgttggcggc gtcgagtgcc ttgggcacgc agccgccgta agcgatcacc gtgacgtccg
    58921 atccggtgcg ccggaccgcg gcgctcgaga tgtcggtggg cgtcaacgta tcgacgtcgg
    58981 ctgcggcgtt gtacagctgg acgtgttcga agatgagcac cgggtccggg tcggcgagcg
    59041 ccgagggcag catgccgtag gcgtcggcca ccgtcgccgg agcgaccacc ttgattcccg
    59101 gaacatgtgc gtaccagcat tccaggctgt gcgagtgctg ggcggccagc tgccggccgg
    59161 cgccggtggc catccgcacg accagcggca cggagaactg gccgccggac atatgccgaa
    59221 gtgccgca
//
LOCUS       DASWBF010000092         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_336450,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000092 DASWBF010000000
VERSION     DASWBF010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336450"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1114
                     /locus_tag="VF874_03220"
     CDS             47..1114
                     /locus_tag="VF874_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX9175129.1"
                     /translation="MTDVISVDLGLTAASGVPTADQPQKAKDFTSDQEVRWCPGCGDY
                     VILNTIRNFLPELGLRRENMVFVSGIGCSSRFPYYLETYGLHSIHGRAPTLATGLALA
                     REDLSVWVVTGDGDALSIGGNHLIHALRRNVNITILLFNNRIYGLTKGQYSPTSEVGK
                     VTKSTPMGSLDQPFNPVSMALGAEASFVGRALDSDRKGLSEVLRAAAAHRGAAFVEIL
                     QDCPIFNDGSFDLLRKEGAEERIVNVAQGQPITFGANGEYCVVRSGFGLEVAKTADVA
                     ASQIMVHDATVEDPSYAFALSRLSDQDLEHTVMGVFRQVQRPSYDDSAREQVHAAREA
                     TPHDTAALQSLLRGRDTWTVD"
     gene            1107..1685
                     /gene="mobA"
                     /locus_tag="VF874_03225"
     CDS             1107..1685
                     /gene="mobA"
                     /locus_tag="VF874_03225"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX9175130.1"
                     /translation="MTKTGSTLAAVVLAGGASRRMGRDKATLPYLGGTLVEHVVGAVS
                     QRCAPVFVIAAPGQRIPAMDAEVLRDDVRGVGPLVATGRGLRAAAEAGVPWAFVCGVD
                     MPFLDTALIDVLVDLSKNLSADVVLPWDGRDHYLAGLYRTSLVGRINELVAAGERSMA
                     ALVNSVEAQRIVVADCRSLTNVNSAADLSVLR"
BASE COUNT          285 a          563 c          603 g          305 t
ORIGIN      
        1 gccaagctgg acggtagcgt gtcgataagt atgcgggagc tagtgcatga ctgatgtgat
       61 tagcgtcgat ctggggctca ccgccgcgag cggtgtgccc acggccgacc aaccgcagaa
      121 ggccaaggac ttcaccagcg accaagaggt ccgctggtgc cccggctgcg gtgactacgt
      181 catcctgaac acgattcgta acttcctgcc ggagctggga cttcggcgcg agaacatggt
      241 gttcgtcagc ggcatcgggt gctcgagccg cttcccgtac tacctggaaa cgtatgggct
      301 gcactcgatc cacggccgcg cgccgacgct ggcgaccggc ctggcgctgg cccgcgagga
      361 cctgtcggtg tgggtcgtca ccggcgacgg cgacgcgctc tcgatcggcg gcaaccacct
      421 gatccatgcg ctgcgccgca acgtcaacat cacgatcctg ttgttcaaca accggattta
      481 cgggttgacc aaggggcagt actcgccgac gtcggaagtc ggcaaggtca cgaagtccac
      541 cccgatgggt tcgctcgacc agcctttcaa cccggtgtcg atggcactgg gcgcggaggc
      601 ctcgttcgtc ggccgcgcgc tggactccga ccgcaagggg ctctccgagg tgctgcgggc
      661 cgctgccgcg catcgcggcg ccgcgttcgt cgagatcctg caggactgcc cgatcttcaa
      721 cgacggctcg ttcgacctgc tgcgcaaaga gggcgccgag gagcggatcg tcaacgtcgc
      781 gcaaggccag ccgatcacgt tcggcgccaa cggcgaatac tgcgtggtgc ggtccgggtt
      841 cggtctcgag gtggccaaga ccgccgacgt ggctgcctct cagatcatgg tgcacgacgc
      901 caccgtcgaa gatccgagct acgcgttcgc gctgtcgcgg ctctccgacc aagacctcga
      961 gcacaccgtg atgggtgtct tccggcaggt gcagcgcccc tcctacgacg acagcgctcg
     1021 cgaacaggtg cacgccgcgc gggaggcgac tccacacgac accgccgcac tgcagtcgct
     1081 gctgcgtgga cgcgacacct ggactgttga ctaaaaccgg ttccacgctg gccgccgtcg
     1141 tgctggcagg tggcgcgtcg cgtcggatgg gccgggacaa ggccacgttg ccgtacctgg
     1201 gcggaacgct ggtcgagcat gtggtcggcg ccgtcagcca gcggtgtgcg ccggtattcg
     1261 tgatcgccgc accgggtcag cgaattcctg cgatggacgc cgaggtgctg cgtgacgacg
     1321 tgcggggggt cgggccgctg gtcgcgactg gccgcgggtt gcgcgcggcg gcggaagccg
     1381 gtgtgccgtg ggccttcgtc tgcggggtcg acatgccgtt tctggacacg gcgttgatcg
     1441 acgtgctggt ggacttgtcg aagaacctga gcgccgacgt ggtgctgccg tgggacggcc
     1501 gcgaccacta cctggcgggg ttgtaccgga cgtcgctggt cggccggatc aacgagctgg
     1561 tcgccgcggg cgaacgcagc atggcggcct tggtgaattc cgttgaggca cagcgaattg
     1621 tggtggcaga ctgccgctcg ctcaccaacg tcaattcggc tgccgatttg tcggtgctcc
     1681 gataacaacc acttttctcc tgctcgcgca aaggagaccc ccgcctcgcg cacgacgaat
     1741 ttgacgcgtc ataaaa
//
LOCUS       DASWBF010000093         7246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_9496, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000093 DASWBF010000000
VERSION     DASWBF010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9496"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..765
                     /locus_tag="VF874_03230"
     CDS             367..765
                     /locus_tag="VF874_03230"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9175131.1"
                     /translation="MKDSADAVIRNYFASFVKSDVDELTSFFTDDAVYVDGPRGVHRG
                     IDAIRAEFEAIVQIVPSTTVDIKALVANGGTVMVERVDNFEIGGKPFDLEVVGVFDVD
                     DNGRITRWHDYYDLRSSEERIAAGLAAQSR"
     gene            complement(753..2321)
                     /locus_tag="VF874_03235"
     CDS             complement(753..2321)
                     /locus_tag="VF874_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9175132.1"
                     /translation="MAATKASRATEEPAKRTATKTPAKRTSANASAPAKRATKATKAT
                     KSTAAKAPAKTTAKATAKAATKPRPSRAKAEKAQAAAPTRSRSKKAAAPEPVEAKAAP
                     VDADETVDELAVDIELEDAEPGDDIVVDEADLVLDDLEEVAEDDVEVVVEADEAEDEA
                     EPEAEAATAEAGGETPAAEDDEIAEPSEKDKASGDFVWDEEESEALRQARKDAELTAS
                     ADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYATQLMTEKADRGEKLPVQQRRDMA
                     WICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKG
                     YKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPSPEE
                     LAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLL
                     QDQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKL
                     RHPSRSQVLRDYLD"
     gene            complement(2484..3308)
                     /locus_tag="VF874_03240"
     CDS             complement(2484..3308)
                     /locus_tag="VF874_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9175133.1"
                     /translation="MTATDTPTTEAGNGAGSHRRGFGVDVGGSGVKGGIVDLETGELI
                     GDRYKLPTPQPATPDAVAKTIAAVVREFGWTEPLGVTYPGVVTNGVVRTAANVDKSWI
                     GIEAAKVFSSALDGQSVTVLNDADAAGLAEEKFGAGKDNTGVIVLLTFGTGIGSAVIH
                     NGVLLPNTEFGHLEVGGMEAEHRAASSVKEKNAWSYKRWAPEVTKVLVAIENAIWPDL
                     FIAGGGISRKAEKWIPLLTCRTPVVAAALQNTAGIVGAAMASQVDATRPIAPLSGV"
     gene            3411..4274
                     /locus_tag="VF874_03245"
     CDS             3411..4274
                     /locus_tag="VF874_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9175134.1"
                     /translation="MTVSDIDTAELRSVAEQLAVEAAEFVQVRRVEVFGDGRPQSGDA
                     AVESKSTRTDPVTVVDTETELLLRDRLAQIRPGEPVLGEEGGGPRQDSDGGSVTWVLD
                     PIDGTVNFVYGIPAYAVSVAVQRGGVSVAGAVADVTRAVVYSAGLGLGARVVDASGMS
                     ETLRCNAIDDLSMALVGTGFGYSPQRRAAQAAVLAGVLPHVRDVRRMGSAALDLCMVA
                     AGCLDAHYEHGLNRWDWAAGALIAAEAGARVQLPRTDGPELTLVAAPGIAADLRDLLE
                     RLGAHDELPED"
     gene            complement(4271..4924)
                     /gene="cei"
                     /locus_tag="VF874_03250"
     CDS             complement(4271..4924)
                     /gene="cei"
                     /locus_tag="VF874_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope integrity protein Cei"
                     /protein_id="HEX9175135.1"
                     /translation="MVAQITEGTAYDRHGRPFRRRNYMPAIIVGAALFVVTAIVWVIA
                     MTRPSDVHEVTACNPPPGRSDPSQPTLGEQVSRANMTDVAPAKLTDTKIRVLNASGQG
                     GQAATVAGALRDLGFGQPTAENDPVYANTKLVCQGQIRFGRAGQATAAAVWLVAPCVE
                     LFQDQRPDDTVDLALGTEFKTPSHSDDIDAMLASLRPEATTAPDLSMLPKIHNSDAC"
     gene            5091..5393
                     /locus_tag="VF874_03255"
     CDS             5091..5393
                     /locus_tag="VF874_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HEX9175136.1"
                     /translation="MATDYDAPRRTETDDVSEDSLEELKARRNEAQSAVVDVDESESA
                     ESFELPGADLSGEELSVRVIPKQADEFTCSSCFLVHHRSRLASEKGGRMICTDCAA"
     gene            complement(5390..5914)
                     /locus_tag="VF874_03260"
     CDS             complement(5390..5914)
                     /locus_tag="VF874_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005057247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HEX9175137.1"
                     /translation="MDTTPKTTLGRVSGSPATSQAVRYRERLTVPWWWWPPAFGVAAL
                     VALEVNRAARDLPGWISFAVLCVIAAGVLIWFGRAEVRVAVGDDGTPVLWAGPAHLPA
                     EVVSRSAEIPQSAKSAALGRQLDPAAYLLHRPWVGPMVLLVLDDPDDPTPYWLVSSRH
                     PERVLAALRESPAG"
     gene            5907..6371
                     /gene="dut"
                     /locus_tag="VF874_03265"
     CDS             5907..6371
                     /gene="dut"
                     /locus_tag="VF874_03265"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX9175138.1"
                     /translation="MSTRLAVVRLDRDLPMPSRAHDGDAGVDLYSAQDVELGPGERAL
                     VPTGIAVAIPYGMVGLVHPRSGLAARVGLSIVNSPGTIDAGYRGEIKVALVNLDPRTP
                     ITVHRGDRIAQLLVQRVELPELIEMSSFDEAGLAQTTRGDGGHGSSGGHASL"
     gene            6372..7058
                     /locus_tag="VF874_03270"
     CDS             6372..7058
                     /locus_tag="VF874_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HEX9175139.1"
                     /translation="MAEGKSREHAVDPDVDGPFDIDDFDDPAQAEVGRLNLGSVLIPV
                     QETGQLQVELTDAGVPSAVWIVTPNGRFTIAAYAAPKSTGLWREVATELADALRKDVT
                     KVSIETGPWGREVVGRGTGTVRFIGVDGYRWMIRCVVNGSDDTIDALADDARKALADT
                     VVRRGETPLPVRTPLQVELPEAIAAQLRAAQQAAAEQGQQEIEPPQPAARRAAPGSAM
                     QQLRTTMGGA"
BASE COUNT         1115 a         2354 c         2465 g         1312 t
ORIGIN      
        1 catagcaaca ctattcgcgt gttacccgcg ccgcagccgc aagacacaat tagataacga
       61 agttctggcg gggaacacta ggcggtatgc caggtcgacc agcgtggtgt agtcgatcgc
      121 cggtcgccgt tcggacaggt aggccacgag caggtctcgc cgggcatcct cgcgaccatc
      181 tcgcggcgaa tgaggttgcc gatgctggtt ggagagcgca ggccgaccac ccgcgccgcg
      241 gcggcaagcc ccaagcaatg acctgtgggt gcgtagcaaa cacaaggtag cttttggaaa
      301 ccccgctcac taggtcctgg cgacatagac ttaggccgcc attgcgtctg cttgaaaggt
      361 tcaaccatga aggattcggc ggatgcggta atacgaaatt acttcgcctc ctttgtgaaa
      421 tcggacgtgg acgaactcac gagctttttc accgacgacg ccgtctacgt cgacggccca
      481 agaggagtcc atcggggaat cgacgccatc cgagccgaat tcgaagcgat tgtgcagatc
      541 gtgccgagca cgaccgtcga catcaaggcg ctggtcgcca acggtggcac agtcatggtt
      601 gaacgagtgg acaacttcga aatcggtggc aagccattcg acttggaagt cgtcggggtg
      661 ttcgacgtcg acgacaacgg ccgcatcacg cgctggcacg attactacga cttaaggtcg
      721 tcagaggagc gcatcgcagc cggactggcc gctcagtcca ggtagtcgcg caggacctga
      781 gaacggctgg ggtgccgcag ttttgacatc gtcttgctct cgatctggcg gatccgctcg
      841 cgggtgacgc cgtacacctg gccgatttcg tccagcgtcc gcggttgccc gtcggtcagg
      901 ccgaaccgca gccgtaccac gccggcctcg cgctccgaca gcgtctccag caccgactgc
      961 agctgatcct gcagcagcgt gaacgagacc gcgtcgacgg cgaccaccgc ctcggagtcc
     1021 tcgatgaaat cgccaagttg cgaatcgccc tcgtcgccga tggtctggtc cagcgagatc
     1081 ggctcacgcg cgtattgctg gatctcgagt accttttcgg gcgtgatgtc catttccttg
     1141 gcgagttctt cgggcgaggg ctcgcgaccc agatcctgca gcagctcacg ctgaatgcgg
     1201 cccagcttgt tgatcacctc gaccatgtgc actgggatgc ggatggtgcg agcctggtcg
     1261 gccatcgcgc gggtgatcgc ctgccggatc caccaggtgg catacgtcga gaacttgtat
     1321 ccctttgtgt agtcgaactt ttcgaccgcg cggatcaacc caaggttgcc ctcctgaatg
     1381 aggtccagga acgccatgcc gcggccggtg tagcgcttgg ccagcgacac caccagtcgc
     1441 aggttcgcct cgagcaggtg gttctttgcc cgatcgccgt cgcggcagat ccatgccatg
     1501 tcgcggcgct gctgtaccgg caacttctcg ccgcggtcag ccttctcggt catcagctgg
     1561 gtggcgtaca ggccggcctc gatgcgcttg gccagctcca cttcctcttc ggcgttgagc
     1621 agcgccacct tgccgatctg cttgaggtag gcgcgtaccg agtcggccga cgcggtgagc
     1681 tcggcgtcct tgcgggcctg gcgcagcgcc tcggattcct cttcgtccca cacgaagtcg
     1741 cctgaggcct tgtctttctc gctcggctcg gcgatctcgt cgtcctcggc ggccggcgtc
     1801 tcaccgcccg cctccgcggt tgccgcttcc gcctccggct ccgcctcgtc ctcggcctcg
     1861 tcggcttcga cgacaacttc tacgtcgtcc tcggcgacct cctcaaggtc gtcgaggacc
     1921 agatcggcct cgtcgacgac gatgtcgtca ccgggctcgg cgtcctcgag ttcgatgtcg
     1981 acggcgagct cgtcgacggt ctcatcggcg tcaacgggcg cggccttggc ctcgaccggc
     2041 tctggcgctg cggccttctt cgaccggctg cgcgtgggcg ccgcggcctg tgccttctcc
     2101 gctttcgcgc gcgacgggcg gggtttggtg gccgctttcg cggttgcttt ggctgtcgtc
     2161 ttggccggcg ccttcgcagc ggtcgacttc gtcgccttgg tggccttggt cgcacgcttg
     2221 gccggcgcgg acgcattcgc cgatgtccgc ttggcggggg tcttggtagc ggtgcgcttc
     2281 gccggctctt cggtcgcccg gcttgctttc gtcgctgcca cgtactccct ttcgcctctg
     2341 ccgacctcgt caagccttgc gcagggcacg acccagtgcg ggctggctta cgctatgtcg
     2401 agtgtcggcg tgtttcagtc ttgtctatcc gccgctgtct tcgatttgcg gccgccgtcg
     2461 accattgtaa cgacagcgcc ggctcagacc ccgctgagcg gtgcgattgg cctggtcgca
     2521 tcgacctgtg aggccatcgc cgcgccgacg atgcccgcgg tgttctgcag tgccgccgca
     2581 accaccggcg tccggcacgt caacagcggg atccattttt ccgccttgcg gctgatgccg
     2641 cccccggcga tgaacaagtc gggccagatc gcgttctcga tcgccaccaa caccttggtg
     2701 acctccggtg cccagcgctt gtagctccag gcgttcttct ctttcaccga cgaggccgcc
     2761 cggtgctcgg cctccatgcc gcccacctcg agatggccga attcggtgtt gggcaacagg
     2821 actccgttgt ggattaccgc cgatccgatg cccgtgccga acgtcagcag cacgatgact
     2881 ccggtgttgt ctttgcctgc cccgaacttc tcctcagcca gccctgccgc gtcggcgtcg
     2941 ttgagcaccg tgaccgactg gccgtcgagc gcggagctga ataccttggc ggcctcgatt
     3001 ccgatccacg acttgtcgac gttcgccgcg gtccggacca caccgttggt caccacgccc
     3061 ggataggtga cgcccagcgg ctccgtccag ccgaactcgc ggacgacggc ggcgatggtc
     3121 ttggccaccg cgtcgggcgt ggccggttgg ggcgtcggca gcttgtagcg gtcgccgatc
     3181 agctcgccgg tctccaggtc gacgatgccg cccttgacgc cgctgccgcc gacgtccacg
     3241 ccgaacccac ggcgatgcga cccggcgccg tttccggctt cggtggtcgg ggtgtcagtg
     3301 gcggtcatga gcactcctcg gcgaggtgac ggcagtcgac gcgaccccac cctactgtct
     3361 gcagacgtgt gcggtggcgc tagtggcgat tcgcatgctg cgatgttgcg gtgaccgtat
     3421 ccgacatcga cacggccgaa ctgcgctcgg tggctgagca actggcagtg gaggccgcag
     3481 agtttgtgca ggtgcgccgc gtcgaggtct tcggtgacgg ccgaccgcaa tccggtgatg
     3541 cggcggttga gtcgaagagc acccggaccg atccggtgac cgtggtggac acggaaaccg
     3601 agcttctgtt gcgcgaccgg ttagcgcaga ttcgaccggg tgagccggtg ctcggcgagg
     3661 agggcggcgg accgcgacag gacagtgatg ggggatcggt gacctgggtg ctcgacccga
     3721 tcgacggaac ggtcaacttc gtctacggaa ttccggcata cgcggtgtcg gtggccgtcc
     3781 agcgcggcgg cgtgtcggtg gccggtgcgg tggccgacgt cacccgagct gtggtgtact
     3841 cggccggctt aggactcgga gcgcgcgttg tcgatgcctc gggcatgtca gagacgttgc
     3901 gctgcaacgc catcgacgat ctttcgatgg cgcttgtggg tacgggcttc ggctattctc
     3961 cacagcggcg cgcagcgcag gcggcagtgc tggcaggggt gctgcctcat gtgcgcgatg
     4021 tccgccggat gggctcggcc gccttggacc tgtgcatggt ggccgccggc tgcctggacg
     4081 cccactacga acacggactc aaccgctggg actgggcggc cggtgcgctg atcgcggccg
     4141 aggcgggcgc gcgggttcag ctgccgcgga cggacgggcc ggagctcacg ctggtcgccg
     4201 caccgggcat cgccgccgac ctacgcgacc tgctcgagcg gctcggcgcg cacgacgagc
     4261 tgccagagga ctagcacgcg tcgctgttgt ggatcttggg cagcatggac aggtccggcg
     4321 cggtggtggc ttccggtcgc aggctggcca gcatcgcgtc gatgtcgtcg ctgtggctgg
     4381 gtgtcttgaa ctcggtgccc aacgccaagt cgaccgtgtc gtcgggccgc tgatcctgaa
     4441 agagctcgac acacggcgcc accagccaga cggccgccgc ggtcgcctgc ccagcgcggc
     4501 cgaacctgat ctgtccctgg cacacgagct tggtgttggc gtacaccggg tcgttctccg
     4561 ccgtgggctg gccgaatccg agatcgcgca gcgcgcccgc caccgtggct gcctgcccgc
     4621 cctgcccgct ggcgttgagt acccggatct tggtgtcggt gagcttggcc ggtgcgacgt
     4681 cggtcatatt ggcgcgagac acctgctcgc ccagggtcgg ttgcgacggg tcgctgcgtc
     4741 cgggtggcgg gttgcatgcg gtgacctcgt ggacgtcgga ggggcgggtc atcgcgatca
     4801 cccagacgat cgcggtgacg acgaagaggg cagccccgac gatgatggcg ggcatgtaat
     4861 tgcggcgccg aaacggccgt ccgtgccggt cataggcggt gccttcggtg atttgcgcga
     4921 ccacccctgc actgtagagg cagcgatcga cacgtttcgc gatgccccgg taacgagtgt
     4981 gacgtaaatc acacttgaac actccgcatt ccgggcacga aaagtttggt ggatgcgttc
     5041 ggtgctggta caaagctctg ctgcggccag tagataaggg ggcaaagact atggctacgg
     5101 attacgacgc ccctcggcgt accgagaccg atgatgtgtc cgaggactcg ctcgaggagc
     5161 tgaaggcccg gcgcaacgag gcgcagtcgg cggtcgtcga cgtcgacgaa tcggagtcgg
     5221 ccgaatcctt cgagctgccc ggcgcggacc tgtccggtga agaactgtcg gtgcgagtca
     5281 tcccgaagca ggctgacgag ttcacctgtt cgagctgctt cttggtgcac catcgcagcc
     5341 gtctggccag tgagaagggc ggccggatga tctgcaccga ctgcgccgcc taaccggcgg
     5401 gcgactctcg cagcgcggcg agtacccgct cagggtggcg gctgctcacc agccagtacg
     5461 gcgtcgggtc gtcgggatcg tcgagcacca gcagcaccat tggaccgacc cagggtcgat
     5521 gcagaaggta ggcggcgggg tcgagctggc gtcccagcgc cgccgacttc gccgactgcg
     5581 gaatctccgc ggagcggctg accacctcgg cgggcaggtg cgccgggccg gcccacagca
     5641 ccggtgtgcc gtcgtcgccg acggccaccc ggacctcggc gcggccgaac cagatcaaca
     5701 cgccggcggc gatcacacac aacacagcaa acgaaatcca cccgggcagg tcgcgggccg
     5761 cccggttgac ctccagcgca accaacgcag ccacgccgaa tgcgggcggc caccaccacc
     5821 acggcaccgt caaccgttcg cggtaacgca cggcttgcga cgttgcgggg ctgcccgaca
     5881 cgcggccaag agtagtcttt ggggtcgtgt ccacccgtct ggcggtcgtg cgcttggacc
     5941 gcgacctgcc gatgccgagc cgagcccatg acggcgacgc gggcgtcgac ttgtacagcg
     6001 cacaagacgt cgagctcggt cccggcgagc gggcgcttgt gcccaccggc atcgccgtgg
     6061 cgatcccgta cgggatggtg ggacttgtgc atccccgatc gggattggct gcccgcgttg
     6121 gactttcgat cgtcaatagt cccggtacga tcgatgcggg ctaccgcggg gaaatcaagg
     6181 tggcgctggt caacctcgac cctaggacgc cgatcaccgt gcatcgcggc gaccgaatcg
     6241 cccagctgct ggtgcaacgg gtcgagttgc cggagttaat cgaaatgtcg tcgttcgacg
     6301 aagccggcct ggcgcagacc acgcgcggtg acggcgggca cggctcttcc ggaggacatg
     6361 cgagcttata gatggcagaa ggcaaatccc gagaacacgc tgtcgatccc gacgtcgacg
     6421 gcccgttcga catcgacgac ttcgacgacc cggcccaggc ggaggtcggg cggctgaacc
     6481 tgggctcggt cctgatcccc gtgcaggaaa caggccagct ccaagtcgaa ctcaccgatg
     6541 ctggcgtgcc gagcgcggtg tggatagtca cccccaacgg ccggttcacg atcgccgcct
     6601 acgcggcgcc gaagagcacc gggctgtggc gcgaagtcgc caccgaactg gcggacgccc
     6661 tgcgcaagga cgtcaccaag gtgtcgatcg agacgggccc gtggggccgc gaggtggtcg
     6721 gtcgcggaac cggcaccgtg cgtttcatcg gggtcgacgg ctatcgctgg atgatccgct
     6781 gcgtggtcaa cggctcggac gacacgatcg acgccctggc cgacgacgcg cgaaaagcgt
     6841 tggcggacac agttgttcgc cgtggtgaga ccccgcttcc ggtgcgcacg ccactgcagg
     6901 tggaattgcc cgaggcgatc gccgcgcagt tgcgcgccgc ccagcaagcg gccgccgaac
     6961 agggccagca ggaaatcgag ccgccgcagc cggcggcgcg gcgcgccgcg ccggggtcgg
     7021 cgatgcagca gctgcgcacg acgatgggcg gggcctaggc ccaatgccgg gtagttaagg
     7081 cccgagggct gttgtcaacg acttcggttc gcgtgtcgtg tgtttcggtt cggggtcgtt
     7141 gatggtggcg gtgatgctgg tgatccgggt gctggggtcg ggccggtagg ggtctttctt
     7201 gttatagcgc gagagcggag atttgacctt gcggacgctg gtgcgt
//
LOCUS       DASWBF010000094         6269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_597220,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000094 DASWBF010000000
VERSION     DASWBF010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597220"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(775..930)
                     /locus_tag="VF874_03275"
     CDS             complement(775..930)
                     /locus_tag="VF874_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175140.1"
                     /translation="MPGDIGVPADAPVGAAIAVPGGIGPAGPEVVMVGAGGGGGGGVG
                     ARGTSEP"
     gene            929..1888
                     /locus_tag="VF874_03280"
     CDS             929..1888
                     /locus_tag="VF874_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175141.1"
                     /translation="MLTTSGVAATGTGAALVSSQTNDPLLDKAVRLAYELLHASRLYP
                     GVHWCVAVFKTANGTETVITSNDGAGYIPPGVFIPRTAKHLFSDPLLDNAFQAKWFGW
                     VNPSATMVAYAAHRAVFDPNYDLYALAATTDPGGSSVLPARRAGVPHYQDCDSTQSPI
                     APDDPAPELDATRLHRLAVIDPQSYAQLRGESQERQAWDATAEAVGMAMAGAEPLFVE
                     VAPAVREIFGALAADITVNDSQWTALDRLQRDRKSLWMRPGYIDMEPSTTSGVTALYR
                     AHHNIDRAVEALSLWRTKDYADIVYAARQVTKEAQLWPTATSS"
     gene            1864..2187
                     /locus_tag="VF874_03285"
     CDS             1864..2187
                     /locus_tag="VF874_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175142.1"
                     /translation="MADRDIKLDAAETLREVEAIAETVRGVAVPGPVPVAAAGTPIDD
                     ALAKVATAQQAQADAWAAALEATVPEQQARSIHAVQMLQDTEQHNSAEIASVYPEGEP
                     GVRNV"
     gene            2214..3158
                     /locus_tag="VF874_03290"
     CDS             2214..3158
                     /locus_tag="VF874_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9175143.1"
                     /translation="MRALVTGAAGFIGSTLVDRLLNDGHQVVGVDNLSSGRRSNLEQA
                     AQNPNFTWIEDDIVTADLDGLLAQHQPEVVFHLAAQIDVRESVADPIHDAKVNVIGTI
                     RLAEAARKANVRKVVHTSSGGSIYGTPHNFPTNESTNTDPASPYAAGKVAGEIYLNTF
                     RHLYGMDCSHIAPANVYGPRQDPHGEAGVVAIFSKALLSGKPTRVFGDGSNTRDYVYV
                     DDVVDAFVKASGPKGGGQRFNIGTGVETSDRKLHSAVAKAVGAPDDPEFAPERLGDLK
                     RSCLDVRLAEMVLGWRPHVRLGEGVARTVDYFRQNPSV"
     gene            complement(3155..3493)
                     /locus_tag="VF874_03295"
     CDS             complement(3155..3493)
                     /locus_tag="VF874_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX9175144.1"
                     /translation="MMWIQVLVIVAVITLLVFLLRSRGSVQARAWVKVGYVVFVIAGI
                     YAVLRPDDTTVLAHWLGVGRGTDLMLYALVVAFAFTSLTTYLHFRDVELRYTRLARAI
                     ALSNAQRPSA"
     gene            complement(3490..4191)
                     /locus_tag="VF874_03300"
     CDS             complement(3490..4191)
                     /locus_tag="VF874_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9175145.1"
                     /translation="MCIDSRYPDVWIVVPAFNEAAVIGDVIADVRATFDHVVCVDDGS
                     RDDTGALALRAGAHLVRHPVNLGQGAAIQTGVEYVRRQPGAEVFVTFDADGQHRVKDV
                     LRMIDKLITEDVDIVIGTRFAAHGMRSDVPFLKRIVLRTAVFLSPRSRRLKLSDAHNG
                     LRVFNRKVAEGLDITMSGMGHASEIIAMIDENHWRVTEEPVEILYTDYSKSKGQPLLN
                     GVNIVFDGFLRGRKS"
     gene            complement(4203..5468)
                     /locus_tag="VF874_03305"
     CDS             complement(4203..5468)
                     /locus_tag="VF874_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175146.1"
                     /translation="MTDAGIPAGPITRGSVARVGVATAVTALCGYLVLYLAARDLDPS
                     GFSVFGVFWGAFGLVTGAANGLLQETTREVRSARHADGAPGVHPLRVAAVIGVVAGLV
                     IACSARIWAPHVFAEARLLSVVLLSVGLAGFCLHATLLGMLAGAGRWTQYGFLMVTDA
                     STRVAVAAATFAVSWGLVGYLWATVCGAVAWLVLLVASPSTRAAARLVACGDTAAFLR
                     GAAHAIAAAGASAILVMGFPVLLKATSGDLGATGGVVILAVTLTRAPLLVPLTAMQGN
                     LIAHFVDQRADRFRALAGPSAVVLGLGFLGVLMAWLAGPWLLRVGFGAEYDADGALLA
                     WLTAAAVSIALLTLTGAAAVAAALHRAYSLGWIGATVASTLLLLLPLELETRTVVALL
                     CGPLVGIVVHLAALRMAPQTGPVSLPPAP"
     gene            complement(5465..6094)
                     /locus_tag="VF874_03310"
     CDS             complement(5465..6094)
                     /locus_tag="VF874_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9175147.1"
                     /translation="MSHGQLPGVFGDVQPVLEHYGYLAVGGFVLLEDFGVPVPGEAIL
                     ITAAIFAGTGHMNIALVIVVAVLGAVIGDNIGFTVGHFGGRPLAERFGRYVFLTPERL
                     GRAEDYFNLHGGKIVVIARFVEGLRQVNGILAGTVGMHWLKFLGFNALGAVLWVCTWA
                     FLGYFAGENIGEIYAGFERYKWYVIGAAAVLVVAYVVVRVDRRRAAETT"
BASE COUNT         1246 a         2339 c         1805 g          879 t
ORIGIN      
        1 ccaaacggcg acaaaaccac cgacaggaac gtcgcaggtg agtggtctga acttctttag
       61 taatcaagac atcccactca agcctgttca cggttcatca aacgacttac cacctgttgg
      121 aggtgaaagg acgacgcccc aactaccagc gactatgcca gtaaattcat ctgcagctaa
      181 tccatcagtg cgcgatcaac gcgggtctgg cgcatcccaa tcatccgacc tcagtcccgc
      241 agatcgtgga actccgattt tttcagctga tcgatcgacc gcggaacaaa cagcaacctt
      301 gccgagtgac actgggcctc catcggttcc catttcgttc ccctcgaatg gctcggttct
      361 gcccggaaat ttcggctcag gcatcggccc tgccggtggt atggctggta tgcctgccgc
      421 aggcgctccg tccctttcgt ccgcgggagt gacgccaccg acgccgtcga ccccatttgc
      481 atccgcccaa cccacaggcc ccgcggccgc tgagttgtct cgcggcctca gccaggggct
      541 gaacactggc gcagctagcg ctccaatcgc cccatcacaa ccgctaattc acccctcgac
      601 agcaccgcca cctaccttca ccgatgccgc cgcagccgct acgcccccag tctccgcagc
      661 gcccgctgcc gccccagcca ctccggtcca agcccctcaa ccgaccgcat accaggtccc
      721 ccaagccgca cccatcatcg ccgcgccgcc gataccgcca agcaacctca cgccctacgg
      781 ctccgatgtg ccccgcgcgc cgacaccgcc gcccccacca ccgccggccc ccaccatcac
      841 cacctccgga ccagccgggc cgatgccacc cggtaccgcg atggcagcgc cgacgggcgc
      901 gtcagccgga actccgatgt ctcccggcat gctgaccacc tccggcgtcg ccgccaccgg
      961 aacgggcgcc gcactcgtca gctcgcagac caacgacccg ctactcgaca aagccgtccg
     1021 cctggcctac gagctgctcc acgcctcgcg cctgtacccg ggcgtgcact ggtgcgtcgc
     1081 cgtcttcaag accgccaacg gaaccgagac cgtcatcacg agcaatgacg gcgcaggcta
     1141 tatcccgccc ggtgtgttca tcccccgaac agcgaaacac cttttctcag accctttgtt
     1201 ggacaacgca ttccaagcga agtggttcgg ctgggtcaac ccgtcggcga cgatggtcgc
     1261 ctacgccgcg caccgggctg tcttcgaccc gaactacgac ctgtacgcac tggccgccac
     1321 caccgacccc ggcggctcct ctgtgcttcc tgcccggcga gccggagtcc cgcactacca
     1381 ggattgcgac tccacccagt cgccgatcgc gcccgacgac cccgcccccg aactcgacgc
     1441 cacccggctg caccgcctcg ccgtcatcga cccccagagc tacgcccaac tcagaggcga
     1501 aagccaagaa agacaggcgt gggatgccac cgctgaagcc gtcggcatgg ccatggccgg
     1561 cgccgaaccg ctgttcgtcg aagtcgcccc cgcggtccgc gagatcttcg gggcgctagc
     1621 cgccgacatc acggtcaacg actcgcaatg gacggcgctc gaccgcctcc aacgcgatcg
     1681 caagtcgctc tggatgcgcc cgggctacat cgacatggaa ccctccacca catcgggcgt
     1741 caccgccctg taccgcgcac accacaacat cgatcgcgcc gtcgaggcac tctccctgtg
     1801 gcgcacgaag gattacgccg acatcgtcta tgccgcaaga caagtcacca aggaggcgca
     1861 actatggccg accgcgacat caagctagac gccgccgaga cactcagaga ggtcgaggcc
     1921 atcgccgaga ccgtcagagg cgtcgccgtc cccggaccgg tgcctgtcgc cgctgcaggc
     1981 accccgattg acgacgccct cgccaaagtc gcgacggctc agcaggcgca agccgacgcc
     2041 tgggcagcag cgctcgaagc caccgttccc gaacaacagg ccaggtccat ccacgcggtg
     2101 cagatgctcc aagacaccga acagcacaac agcgccgaga tcgccagcgt gtaccccgaa
     2161 ggggagcccg gcgtccgcaa cgtttagcaa accgccgtcg gtatggtgaa gcggtgcgcg
     2221 cactggtcac cggagctgcc ggattcatcg ggtcgacact ggtcgaccgg ctcctcaacg
     2281 acggccacca ggtcgtcggc gtcgacaacc tgagctccgg ccgaagaagc aatctcgagc
     2341 aggcggcaca gaaccccaac ttcacgtgga tcgaagacga catcgtcacc gccgacctgg
     2401 acggcctgct agcccaacat caacccgaag tcgttttcca tctcgccgca cagatcgacg
     2461 tccgcgagtc cgtggcagac ccgatacacg acgccaaggt caacgtgatc ggcaccatta
     2521 ggctcgccga agccgcccga aaagccaacg tgcgcaaggt cgttcacacg tcatcgggcg
     2581 gttcgatcta cggaacgccc cataatttcc ctaccaacga gtcgacgaac accgatccgg
     2641 catccccgta cgcggccggg aaagtcgccg gcgaaatcta cctgaacacc ttccgccacc
     2701 tgtatggcat ggactgctcg cacatcgccc cggccaacgt ctacggaccg cgccaggacc
     2761 cccacggtga agccggcgtg gtcgcgatct tctcgaaggc gctgttgtcc gggaaaccca
     2821 cgagggtctt tggcgacggg tccaacaccc gcgattacgt gtacgtcgac gacgtcgtcg
     2881 acgcattcgt gaaagcgtcc ggacccaaag gcgggggaca acggttcaac atcggcaccg
     2941 gcgtggaaac ctctgaccgc aaactacatt cggcggttgc gaaggccgtc ggcgcgcccg
     3001 acgaccccga attcgctccc gagcgactcg gcgatctgaa gcggtcctgc ctcgacgtcc
     3061 ggctcgccga gatggtgctc ggatggcgtc cccacgtcag gcttggcgag ggcgtcgccc
     3121 gcaccgtcga ctatttccgg cagaacccga gcgtctaagc cgaaggccgc tgcgcattcg
     3181 acagcgcgat cgcccgtgcc agccgcgtgt aacgcaactc cacgtcccgg aaatgcaggt
     3241 atgtggtcaa cgacgtgaac gcgaacgcca cgacgagcgc gtagagcatc aagtccgtcc
     3301 cgcggccgac ccccagccag tgcgccagca ccgtggtgtc gtcaggccgc aacaccgcgt
     3361 agatgcccgc gatcacgaac accacgtacc cgaccttcac ccaggcccgc gcctgcaccg
     3421 atccgcgtga ccgcagcaag aacaccagca gagtgatcac cgcgacgatc accaggactt
     3481 gaatccacat cacgacttcc ttccgcgcag gaacccgtcg aagacgatgt tgacgccgtt
     3541 cagcagcggc tggcccttcg acttcgaata gtccgtatac aggatttcga ccggctcttc
     3601 ggttacccgc caatggtttt cgtcgatcat cgcgatgatc tcgctggcat gccccatgcc
     3661 gctcatcgtg atgtcgaggc cctcggccac cttccggttg aacacccgca gcccgttgtg
     3721 cgcgtccgac agcttgagcc gccggctgcg cgggctgagg aacaccgccg tgcgcaacac
     3781 aatgcgtttc aagaaaggca catcgctgcg catcccgtga gcggcgaacc gagtgccgat
     3841 gacgatgtcg acgtcctcgg tgatcagctt gtcgatcatc cgcaaaacgt ctttgacacg
     3901 gtgctgcccg tcggcgtcga acgtcacgaa cacctccgcg ccgggctgac gacggacata
     3961 ttcgacgccg gtctgaatcg ccgcgccctg ccccaagttc accgggtgcc gcacgaggtg
     4021 cgctcccgcg cgcaaggcaa gagctccggt gtcgtcgcgg ctaccgtcgt ccacacacac
     4081 gacatggtcg aaggtcgccc ggacatcggc gatcacgtca ccgatgacgg cggcttcgtt
     4141 gaaagcgggg acgacgatcc agacgtcggg gtagcggctg tcgatgcaca taaaaataca
     4201 cgtcagggcg ctggaggcag cgacaccggg ccggtctgcg gcgccattcg caacgccgcc
     4261 agatgcacga cgatgcccac caaaggccca cacaacagcg ccacgaccgt cctggtttcc
     4321 aactccagcg gcagcaagag cagcagcgtc gatgcgacgg tcgcgccgat ccagcccagc
     4381 gaataggccc ggtgcaatgc cgcggcaacc gccgccgcgc cggttaacgt caaaagcgcg
     4441 atggataccg ccgccgcagt caaccacgcc agcagcgccc cgtcggcgtc gtattcagcc
     4501 ccgaacccga ccctgagcag ccagggcccc gccagccaag ccatcagcac accgaggaaa
     4561 ccgagtccga gaaccaccgc agaaggccca gccaacgccc ggaaccggtc ggcgcgctgg
     4621 tccacgaagt gggcgatcaa gttgccctgc atcgcggtca gcggcaccag cagcggcgcc
     4681 cgcgtcagcg tcaccgccaa gatcacgacg ccgcccgtcg cgccgagatc ccccgacgtg
     4741 gccttcagca gcaccggaaa gcccatcacc aggatcgcgc tggcgccggc cgcggcgatc
     4801 gcgtgcgccg cgccgcgcag aaatgccgcg gtgtccccgc aggccaccag ccgcgcagcc
     4861 gcccgcgtcg acggggacgc caccaacaag accagccagg ccaccgcgcc gcacaccgtc
     4921 gcccacagat atccgaccag cccccagctc accgcgaacg tcgccgccgc caccgcgacc
     4981 cgcgtcgaag cgtcggtgac catcaagaag ccgtactgcg tccaccggcc ggcaccggcc
     5041 agcatgccga gcagcgtcgc gtggaggcag aacccggcca ggccaacgct gagcagcacc
     5101 acgctgagaa gccgcgcctc ggcgaacacg tgcggagccc agatccgtgc actacaagcg
     5161 atgaccagtc cggccaccac accgatcacc gccgccaccc gcagcggatg aactcccgga
     5221 gccccgtctg catgcctcgc cgaccggact tcgcgcgtcg tctcctgcag caggccgttg
     5281 gctgcgccgg tgacgaggcc gaacgccccc cagaacaccc cgaacaccga aaacccggat
     5341 gggtccaagt cgcgtgccgc cagatacagc acgaggtagc cgcacaacgc cgtcaccgcg
     5401 gtggcgacac cgacccgcgc gacgctgccc cgggtgatgg ggccggccgg tatccccgcg
     5461 tcggtcacgt cgtctccgca gcccgccggc ggtccacccg gacgacgacg tacgccacga
     5521 cgagcaccgc ggccgcgccg atcacgtacc acttgtaccg ctcgaagccg gcgtagatct
     5581 cgccgatgtt ctcgccggcg aagtatccca ggaaagccca cgtgcacacc cacagcaccg
     5641 cgccaagagc gttgaacccg aggaacttca gccagtgcat cccgacggtc ccggccagga
     5701 tgccgttgac ctggcgtagc ccctcgacga aacgggcgat cacgacgatc ttgccgccgt
     5761 ggaggttgaa gtagtcctcg gcgcggccga gccgttcggg cgtgaggaag acgtagcggc
     5821 cgaagcgctc ggcgagcggg cggccgccga agtggccgac cgtgaagccg atgttgtcac
     5881 cgatgactgc gccgagcacc gcgacgacga tgacgagcgc gatgttcatg tggcccgtgc
     5941 cggcgaagat ggccgccgtg atcaggatgg cctcgcccgg caccggcaca ccgaagtcct
     6001 ccagcagcac gaaaccgccg accgccagat acccgtagtg ctccaggacc ggctgcacat
     6061 cgccgaacac cccaggcagc tgcccatggc tcatgcgtgc tccgtccggt cttcgtcacc
     6121 cctagtgcaa agagaccata cggccgggcc ggcgtcgggc tcggcatgag agactgaaat
     6181 tggaggggtg acgaactgtt gagcaactcg cccgtgaatg tgcgtgtttg tgagtgaatg
     6241 tatgtttgac tggcggtcgt gaagttggc
//
LOCUS       DASWBF010000095         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_929762,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000095 DASWBF010000000
VERSION     DASWBF010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929762"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VF874_03315"
     CDS             complement(<1..459)
                     /locus_tag="VF874_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9175148.1"
                     /translation="MRVQPAAFFRTTLPLDLSQLARLDAGRYHSIWLPDHMVSFWPDS
                     IWTPEFTDLATVSPSPHRNLDGMAVAAAAAVLTRNVPIATSVVDTVRRHPSLLAQSAL
                     TIDHLAKGRFILGLGSGETENTVPYGFDFNKPVARFEEALKVIRLLWDSDG"
     gene            553..1536
                     /locus_tag="VF874_03320"
     CDS             553..1536
                     /locus_tag="VF874_03320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020106137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HEX9175149.1"
                     /translation="MFSAILIEKDDNGQTVARTDLDEAQLPDGDVSIDVEYSTLNFKD
                     GLAITGKSPVVRKFPMVPGIDLAGVVTDSSHADWKTGDAVVLNGWGVGETHWGGLAQR
                     ARLNGDWLVPLPAEFTTRQAMAIGTAGYTASLCVDALLDHGVSADQGEVLVTGATGGV
                     GSVAIALLAKAGFEVAAATGKSSEAEYLEKLGAATVLDRAELAEKGKALQKERWAGVV
                     DTAGSYTLANACAQTHYGGAVAACGLAQGMDFPASVAPFILRGVSLLGVDSVMAPKAR
                     RLAAWKRLSRDLDAGALESIAREIALEEAIAAAGELMDGKVRGRIVVDVNR"
     gene            1549..>2025
                     /locus_tag="VF874_03325"
     CDS             1549..>2025
                     /locus_tag="VF874_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020106138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9175150.1"
                     /translation="MARKPNKPESVELDLSDVDHRVGKPIGGGQLWDPCSSSDIRRWV
                     MAMDYPNPLHWDELFARESKYGGIVAPQSIAVGLDYGHGAAPACVGHIPDSHLIFGGE
                     EWWFYGCPIRPGDKLFQERRFHDYKVVETKFAGPTMFSRGDTVHHNQHGVLVARERS"
BASE COUNT          349 a          638 c          722 g          316 t
ORIGIN      
        1 accgtcgctg tcccagagca gccggatcac cttcagcgcc tcctcgaagc gagcgaccgg
       61 cttgttgaag tcgaagccgt acgggacggt gttctcggtt tcgccgctgc cgaggcccaa
      121 gatgaagcgg cccttcgcga ggtggtcgat cgtgagcgcg ctctgcgcga gaagcgacgg
      181 gtggcggcgc accgtatcca ccacgctggt cgcgatcggc acgttgcggg tcagcacggc
      241 cgccgccgcc gcgaccgcca tgccgtcgag gttgcggtgc ggggacggcg acacggtggc
      301 caggtcggtg aactcgggcg tccaaatcga gtccggccag aagctcacca tgtggtcggg
      361 cagccagatc gagtggtagc ggccggcgtc aagccttgcc agctgagaca ggtccagcgg
      421 cagggtggtg cggaagaacg ctgccggctg aactctcatc gacctagcac ccctgccatg
      481 gaaatatcat gctcaacgct gagaatgata ttctcagcga ccggaccccg gcgtcgagga
      541 atggagccac cgatgttttc ggcaattctg atcgagaagg acgacaacgg gcagaccgtc
      601 gcgcgcactg acctcgacga ggcgcaactc ccggacggag acgtcagcat cgacgtcgaa
      661 tactcgacgc tgaacttcaa ggacggcctc gcgatcaccg gcaagtcgcc ggtggtgcgc
      721 aagttcccga tggttccggg catagacctc gccggcgtcg tcaccgacag cagtcacgcg
      781 gactggaaga cgggcgatgc cgtcgtgctc aacggctggg gcgtcggtga gacacattgg
      841 ggcggactcg cccagcgtgc gcgcctcaac ggcgactggc tggttcccct ccccgccgag
      901 ttcaccacgc ggcaggcgat ggcgatcggc acggccggct acacggcgag cctgtgcgtc
      961 gacgccctgc tggaccacgg cgtgtccgcc gaccagggcg aggtgctggt gaccggagcg
     1021 acgggcggcg tcggcagcgt cgcgatcgct ctgctggcaa aggccggctt cgaggtggcc
     1081 gcggcgaccg gtaagtcgtc ggaagccgag tatctcgaaa agctgggcgc tgcaacggtg
     1141 ttggaccgcg ccgaactcgc cgagaaaggc aaggcgctgc agaaggaacg ttgggcaggc
     1201 gtggtcgaca cggccggcag ctacacgctg gccaacgcgt gcgctcagac gcattacggc
     1261 ggcgcggtcg ccgcgtgcgg gctggctcag ggcatggact tccccgcatc ggtggccccg
     1321 ttcattttgc ggggcgtgtc gctgctcggc gtcgacagcg tgatggcgcc gaaggcgcgg
     1381 cgtttggccg cgtggaaacg tctgtcccgc gacctggatg ccggcgcgct cgagtcgatc
     1441 gcgcgggaga tcgcgctcga agaagccatc gccgcggccg gtgaactgat ggatggcaag
     1501 gtgcgcggcc gcatcgtcgt cgacgtcaac cgctgatcgg agcacgcaat ggccaggaaa
     1561 ccgaacaaac ccgagagcgt cgagctcgac ctgtccgacg tcgaccaccg ggtcggcaag
     1621 ccgatcggcg gcgggcagct gtgggatccg tgcagttcgt cggacattcg tcgctgggtg
     1681 atggcgatgg attaccccaa tccgctgcat tgggacgagc tcttcgcccg ggagtccaag
     1741 tacggcggca tcgtcgcgcc gcagtcgatc gcggtgggcc tggactacgg gcacggcgca
     1801 gcgcccgcat gcgtggggca catccccgac agccacttga tcttcggcgg cgaggagtgg
     1861 tggttctacg gttgtccgat ccggccgggt gacaagcttt tccaggagcg ccggttccac
     1921 gactacaagg tggtggagac gaaattcgct gggccgacga tgttttcgcg cggcgacacc
     1981 gtgcaccaca atcagcacgg cgtgctggtg gcccgcgagc ggtcc
//
LOCUS       DASWBF010000096         7622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_732325,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000096 DASWBF010000000
VERSION     DASWBF010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732325"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..554)
                     /locus_tag="VF874_03330"
     CDS             complement(45..554)
                     /locus_tag="VF874_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX9175151.1"
                     /translation="MVHSIELLVDADTEAAIHRAWDALSDAGLRTPPPTSRPHVTLLV
                     ADEISPDVDAPLSQVLQRLPLACVIGAPIVFGRSPFVLVRLVVPSTELLELHADAARI
                     CSPYLTPAAAPNTGVGQWTPHTTLARRVEPSQLVRALCIRTITRDIRGNVVGMRRWDG
                     LKRVEHLIR"
     gene            complement(556..1236)
                     /gene="bioD"
                     /locus_tag="VF874_03335"
     CDS             complement(556..1236)
                     /gene="bioD"
                     /locus_tag="VF874_03335"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX9175152.1"
                     /translation="MSHLFVTGTGTAVGKTVATAALACCARLAGIDVAVCKPVQTGIT
                     EGDDDLAEVTRLSGVDRVVGSWRYPEALAPVAAAKRAGLPLPNRVELDVFLRVVDHSH
                     QLTLVEGAGGLLVEIGDGGVTLRDLAKDAHGSVVVVVEAGLGTLNHTALTLEALETQD
                     VPCAGLVVGAWPAEPGPVEIGNREALANLAPLRAVLPAGSAAMTREQFEQMSGSAIDR
                     DWLAELAN"
     gene            complement(1233..2384)
                     /locus_tag="VF874_03340"
     CDS             complement(1233..2384)
                     /locus_tag="VF874_03340"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX9175153.1"
                     /translation="MKSRIDMSPLAWLDEVERQRRGAGLRRSLRTRSPVGAEVDLASN
                     DYLGLSQHPAVIDGGVQALRTWGAGSTGSRLVTGNTELHEEFENALAEFVGAPSALVF
                     SSGYAANLAAVVALSGPGSLVVSDAHSHASLVDACRLSRARVVVTPHRDVDAVDAALA
                     SRQEDRAVVVTDSVFSADGALAPLLSLHEVCRRHGALLIADEAHGLGVRGDGGRGLLH
                     EVGLAGAPDVVMTTTLSKALGSQGGVVLGPTAVRDHLIDAARPMIFDTGLAPAALGAA
                     LAALRVLIAEPDRPRTVLRNAAVLADTCRVAERPESAVVSVILGEPEVAVGAAAACVQ
                     RGVRVGCFRPPSVPPGTSRLRLTARASLSDDELDTAQRVLAEVLHTARQ"
     gene            complement(2705..3232)
                     /locus_tag="VF874_03345"
     CDS             complement(2705..3232)
                     /locus_tag="VF874_03345"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX9175154.1"
                     /translation="MIARWWKKMARSAVGALVAALAFATPAAADSGSIRSAKGDGGNA
                     STSPTVPLDEPMAPGLAGTSWRFVEILGVAPPATVEATLEFSAGGAAAGSSGCNRFFG
                     RYSSAGTDLSFSDLGSTKMMCTPEVMAVETAVQTALNRTSHVAGGLDLLASDGTVLAR
                     LDPVTLPPVPAPDEP"
     gene            complement(3335..4621)
                     /locus_tag="VF874_03350"
     CDS             complement(3335..4621)
                     /locus_tag="VF874_03350"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX9175155.1"
                     /translation="MPALTPEQIGAIDAAHVWHPYSPMGEHALAPTVAVAAQGASLTV
                     IRDGRRLDVLDAMSSWWTAIHGHGHPALDTAITDQLATMNHVMFGGLTHEPAARLAQL
                     LVDITPPGLDTVFFSDSGSVSVEVAVKMALQYWRSFGRPTKHRLMTWRGGYHGDTFTP
                     MSVCDPDSGMHVLWTDVLARQVFAPQVPSEYDSSYSAAFEDQLARHAHELAAVIVEPV
                     VQGAGGMRFHDAGYLVDLREMCRRHGVLLIFDEIATGFGRTGELFAADHAGVAPDIMC
                     VGKALTGGYITLAATLCSRDIAQTISSHEPGALMHGPTFMANALACAVSVASVELLLG
                     QDWRSRVRAIESGLRSGLQKAQTVDGVADVRVLGAIGVVEMRQPVDLAVATPVALDHG
                     VWLRPFRNLIYAMPPYICSPDEIAQITSAMVAVAAL"
     gene            4687..6891
                     /gene="glgX"
                     /locus_tag="VF874_03355"
     CDS             4687..6891
                     /gene="glgX"
                     /locus_tag="VF874_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX9175156.1"
                     /translation="MASSKTNRDERWREEPKAADTGPILATVWPGTPYPLGATYDGAG
                     TNFSLFSEVAQHVDLCLIEKDGTEERIPLDEVDGFVWHAYLPAITPGQHYGFRVHGPW
                     EPAAGHRCDPSKLLLDPYGKAFDGDFKRGRALFSYDMAAAQHDDSASVKAPTINSRRR
                     TMTSVVINPFFDWGSDRAPRRPYNETIIYEAHVKGMTQTHPGLPEELRGTYAGLSHPA
                     VIEHLLSLNVTALELMPVHQFMHDFRLLDLGLRNYWGYNTFGFFAPQNSYASIRQPGA
                     AVGEFKTMVRTFHEAGIEVILDVVYNHTAEGNQLGPTINFRGIDNAAYYRLVDEDLRY
                     YKDYTGTGNSLNARHPHTLQLIMDSLRYWVLEMHVDGFRFDLASTLARELHDVDRLSA
                     FFDLVQQDPVVSQVKLIAEPWDVGEGGYQVGNFPGLWTEWNGKYRDTVRDYWRGEPAT
                     LGEFASRLTGSSDLYEETGRRPGASINFVIAHDGFTLHDLVSYNEKHNEANGEGNRDG
                     ESHNRSWNCGVEGPTDDPEIIKLRCRQMRNFLATLMVSQGTPMIAHGDEMGRTQLGNN
                     NVYCQDSKLSWMDWSLTERNADLLEFSRKVTALRKHHPVFRRRRFFEGTPIRSGAQTR
                     DIAWLTPAGQEMTPEDWGSGFGKSIAVFLNGRAIPEPDARGFPVVDDSFLLCFNGHDE
                     DLDFVVPGGIYGSEWEACLDTADPEGNTDLVVRVGDKITLEARSVIVLRRAA"
     gene            6888..>7622
                     /locus_tag="VF874_03360"
     CDS             6888..>7622
                     /locus_tag="VF874_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9175157.1"
                     /translation="MTGCASSPASGRYPHLASAAVVSTYRLQLRGPASGQPFTFADAE
                     NLLDYLDDLGVTHVYLSPILTAVNGSTHGYDVTDPTTVFDELGGANGLARLSAAARSR
                     GMGLVVDIVPNHLGVDAPAQNPWWWDVLRHGRDSAYAPYFDIDWGSDNGVDGKLALPV
                     LGSDDDVADLTLDGDLLRLGDLTFPIAPGTGDGTGPQVHDRQHYRLIGWRRGLCGYRR
                     FFAITSLAGLRQEDRGVFDASHAEVRR"
BASE COUNT         1315 a         2660 c         2496 g         1151 t
ORIGIN      
        1 aagttcgagt gagggcctgg cggaatacag tttcggcgag aaagtcagcg gatgaggtgc
       61 tccacccgct taaggccgtc ccagcggcgc atcccgacga cgttgccgcg gatatcgcgg
      121 gtgatcgtgc ggatgcacag ggctcgcaca agttgcgacg gctcgacccg acgcgccagc
      181 gtggtatgcg gagtccattg tcccacaccg gtattcggcg cagctgcagg tgtgaggtat
      241 ggcgaacaga ttcgcgcggc gtcggcatgc agctccagca gctctgtcga cggcacgacg
      301 agccgcacca agacgaacgg cgagcgccca aacacgatgg gtgcaccgat cacacacgca
      361 agcggcagcc gctgcaagac ctggctcaat ggcgcatcga cgtccggcga aatctcgtcc
      421 gccacaagca atgtcacatg cggccggctc gtcggcggtg gagtccgcaa gccggcgtcg
      481 gacagcgcgt cccacgctcg atgaatcgca gcctcggtgt cggcgtccac caggagctcg
      541 atcgagtgga ccacgtcagt tggccagctc cgccagccag tcccgatcga tggccgagcc
      601 gctcatctgc tcgaactgct ctctggtcat cgccgccgac ccggccggca gcaccgcgcg
      661 caacggcgcc aaattcgcca gcgcctcacg gttgccgatc tcgaccggtc ccggctcggc
      721 cggccaggcg cctaccacca ggccggcgca cggaacatct tgtgtctcaa gcgcttccag
      781 cgtcagcgcg gtgtggttga gcgttccgag cccggcttcg acgacgacga ccacggaccc
      841 gtgcgcatcc ttggccaggt ctcgtagcgt gacaccgccg tccccgatct cgacgagcag
      901 tcccccggca ccctcgacca gcgtgagttg atgcgagtgg tcgaccaccc gcagaaacac
      961 gtccagctcc acccggttgg gcagcggaag ccccgcgcgc ttcgctgcgg cgaccggggc
     1021 cagcgcctcc gggtagcgcc acgatccgac gactcgatcg accccagaga gccgtgtcac
     1081 ttcggcgagg tcgtcgtcgc cttctgtgat gccggtctgc acgggcttgc acaccgccac
     1141 gtcgatgccc gcaagccgcg cgcaacacgc cagcgccgcg gtcgcgaccg tcttgccgac
     1201 agcggtgccg gtgccggtga cgaacaggtg gctcactggc gggcggtgtg cagcacttcg
     1261 gccaacaccc gctgcgcggt gtccagttcg tcgtcgctca gcgacgccct ggccgtcaac
     1321 cgcagccgcg acgtgcccgg cggcaccgac ggcggtcgga aacatcccac gcgcaccccg
     1381 cgctgaacgc acgcggccgc agcgcccacg gccacctcgg gttcgcccag aatcaccgac
     1441 accaccgcgg actcgggcct ctctgccact ctgcaggtgt ccgcgaggac ggcggcgttg
     1501 cgcaacaccg tgcggggccg gtcgggttcg gcgatcagca cccgcagcgc ggccagcgcc
     1561 gcgcccagcg cggccggtgc gagcccggtg tcgaagatca tcggccgggc cgcgtcgatc
     1621 agatggtcgc gcaccgccgt cgggccgagc accaccccgc cctggctgcc gagcgccttc
     1681 gacagcgttg tcgtcatcac cacgtcgggc gctccggcga gccccacctc gtgcagcagc
     1741 ccgcgaccgc cgtcgccccg cacgcccagg ccgtgcgcct cgtcggcgat cagcagcgcg
     1801 ccgtggcgac ggcacacctc gtgcagcgac agcagcggcg ccagggcgcc gtccgcggag
     1861 aacaccgagt cggtgaccac taccgcgcgg tcctcctgcc gtgatgccag cgctgcgtcg
     1921 acggcgtcga cgtcccggtg cggcgtcacc acaacccggg cccgcgacag ccggcacgcg
     1981 tctaccagcg acgcgtggct gtgcgcgtcg gacaccacca gcgacccggg gccggacaat
     2041 gcgacgacgg ccgccaagtt tgcggcgtag cccgacgaga acaccaatgc cgaaggggcg
     2101 ccgacgaatt cggccaacgc gttttcgaac tcctcgtgca gctcggtgtt gccggtcacc
     2161 agccgcgagc cggtcgaccc ggcgccccag gtgcgcagcg cctgcacgcc gccgtcgatc
     2221 accgcggggt gctgcgacag ccccaggtag tcgttggagg cgaggtccac ctcggcgccc
     2281 accggcgacc gcgtccgcaa ggaccggcgc agtcccgcac cccggcgctg ccgctcgacc
     2341 tcgtcgagcc acgccagcgg cgacatatcg atgcggcttt tcatcagcgc ttaccctacg
     2401 caacccgcga cggcggccgc gcttcagtca ccgatgcgtt cgaacctttc ctcgaagtcg
     2461 gtgctgatac agctgctgcc gggagtcgtt atctcgttgt gcccgtgccc gctcaaacag
     2521 tgtgatggga ttctgcggag gcttcctgaa gcgggaattc cgcagtcctc ttgatatgag
     2581 tggggccgct cggtgtggga aacgagcatg aaggacggat cccatctgaa cgtctatgaa
     2641 gtgccggtga gcatgagttg agccgcgtcg tcgaagcgcg cgtgttgttg gcccactcct
     2701 cgagctaagg ttcgtcaggt gccggcaccg gcggcagggt caccgggtcg aggcgcgcca
     2761 gcacagtgcc atccgaggcg agcagatcga gtccaccagc cacgtggctc gtgcgattga
     2821 gagccgtttg cacggccgtc tccaccgcca tgacctccgg tgtgcacatc atcttggtgg
     2881 agccgaggtc ggaaaaagag agatcggtac cagccgagga atagcgaccg aaaaagcgat
     2941 tgcagcccga cgagcccgcc gccgcaccgc ctgccgagaa ttcgagggtc gcctcgacgg
     3001 tggcgggagg agccaccccg aggatctcga cgaagcgcca ggacgtaccg gccaggcccg
     3061 gtgccatggg ctcgtccagc ggcacggtcg ggctcgtcga cgcattgccg ccatcgcctt
     3121 tcgcactgcg gatcgacccc gagtcggccg cagccggcgt cgcgaacgcc aacgcggcta
     3181 ccagtgcgcc gacggccgag cgcgccatct ttttccacca acgcgcgatc acgtcgccaa
     3241 actacacatt ccacggcccg acctgcggga cattgagctg cgtcgattac cctccctagg
     3301 cacccgtgcg ccgatggtga gctcaaggag tgtttcaaag ggcagcgacg gcgaccattg
     3361 ccgaggtgat ctgcgcgatc tcgtcaggcg agcagatgta gggcggcatc gcatagatca
     3421 ggttgcggaa cggccgcagc cacacgccgt ggtccagcgc gacgggcgtg gcgaccgcaa
     3481 ggtcgacggg ctgccgcatc tcgaccacac caatcgcgcc gagtacccgg acgtcggcca
     3541 cgccgtcgac ggtctgcgct ttctgcaggc ccgaccgaag ccccgactcg atcgcgcgca
     3601 cacgggaccg ccaatcctgg ccgagcagca gctccaccga cgccaccgaa accgcacagg
     3661 ccagcgcgtt ggccatgaac gtcgggccgt gcatcagtgc gccgggctcg tggctgctga
     3721 tggtctgcgc gatgtctcta ctgcacaacg tcgcggccag cgtgatgtac ccgccggtca
     3781 gcgccttgcc gacgcacata atgtccggcg cgacaccggc atgatcggcg gcgaacagct
     3841 ccccggtgcg gccgaaaccg gtcgcgatct cgtcgaagat caacagcacg ccgtggcgcc
     3901 ggcacatttc gcgcaggtcg acgaggtagc cggcgtcgtg gaatctcatc ccaccggcac
     3961 cctgtaccac gggttcgacg atcaccgcgg ccagctcgtg ggcgtggcgg gccagttggt
     4021 cctcgaacgc agcggagtac gacgagtcat actcggaagg cacctgcggc gcaaacacct
     4081 gccgcgccag cacgtcggtc cacaacacgt gcatgccgct gtcggggtcg cagacgctca
     4141 tcggggtgaa cgtgtcgccg tggtagccgc cgcgccaggt catcagccgg tgtttggtcg
     4201 ggcggccgaa gctgcgccag tactgaagcg ccatcttgac cgcgacctcc accgacaccg
     4261 agccggagtc gctgaagaac accgtgtcca ggccgggagg ggtgatgtcg acgagcagtt
     4321 gcgcgagccg cgccgccggt tcgtgggtca gcccgccgaa catcacgtgg ttcatcgtcg
     4381 cgagctgatc cgtgatcgcg gtgtccagcg ccggatgacc gtggccgtga atcgccgtcc
     4441 accacgagct catcgcatcg agcacatcga gccgccggcc gtcgcggatc acggtaaggg
     4501 acgcgccctg tgcggccacc gcgaccgtcg gcgccagcgc gtgctcgccc atcgggctgt
     4561 aggggtgcca gacgtgcgct gcgtcgatcg cgccgatctg ctcgggggtc aacgcgggca
     4621 cggagaccga gcctaatgct ccgcccgcga cgggcggtcg tggatagccg ccgatagggt
     4681 cgaatgatgg cctcgagtaa aacgaaccgc gatgagcgct ggcgcgaaga gccgaaggca
     4741 gcggacaccg ggccgatcct ggccacggtg tggccgggca cgccgtatcc gctgggcgcc
     4801 acctatgacg gcgcgggcac caacttctcg ctgttctccg aggtcgccca acacgtagac
     4861 ctgtgcctga tcgagaagga cggcaccgag gagcgcatcc ccctcgacga agtcgacggc
     4921 ttcgtctggc atgcctatct gccggcgatc actccggggc agcattacgg attccgcgtc
     4981 cacgggccgt gggagccggc cgccgggcat cggtgcgatc cgagcaagct gctgctggac
     5041 ccatacggga aggccttcga cggcgacttc aaacggggcc gggcgctgtt ctcctacgac
     5101 atggccgcgg cccagcacga cgactcggca tcggtcaagg cgccgacgat caactcgcgc
     5161 cgtcgcacga tgaccagcgt ggtgatcaac ccgttcttcg actgggggtc agaccgggca
     5221 ccgcggcggc cgtacaacga gacgatcatc tacgaggccc acgtcaaggg catgacgcag
     5281 acccatcccg gcctgcccga agaactgcgc ggcacctacg cagggctgtc ccatcccgcc
     5341 gtgatcgagc acctgctgtc actgaacgtc actgcgctcg agctgatgcc ggtgcaccag
     5401 ttcatgcacg atttccggct gctcgacctg ggcctgcgga actactgggg gtacaacacc
     5461 ttcgggttct tcgcgccgca gaactcctac gcgtcgatcc ggcagcccgg tgccgcggtc
     5521 ggcgagttca agaccatggt gcgcacgttc cacgaggccg gcatcgaagt catcctcgac
     5581 gtggtttaca accacaccgc cgagggcaat cagctgggac ccacgatcaa cttccgcggc
     5641 atcgacaacg ccgcctacta ccggctggtc gacgaggatt tgcgctacta caaggactac
     5701 accggcaccg gcaacagcct gaacgcgcgc catccgcaca cactgcaatt gatcatggat
     5761 tcgctgcgct actgggtgct ggagatgcat gtcgacggct tccggttcga cctggcctca
     5821 acgctggccc gcgaactgca cgacgtcgac cggctgtcgg cgtttttcga tctggtgcaa
     5881 caggacccgg tggtcagcca agtcaagctg atcgccgagc catgggacgt cggcgagggc
     5941 ggctatcagg tgggcaactt ccccggcctg tggaccgaat ggaacggcaa gtaccgcgac
     6001 accgtccgcg actactggcg cggcgagccc gccacgctcg gcgagttcgc gtcacggctg
     6061 accgggtcgt cggacctgta cgaggagacc gggcgccggc ccggcgccag catcaacttc
     6121 gtcatcgcac acgacggttt cacgctgcac gacctggtgt cctacaacga gaagcacaac
     6181 gaggcgaacg gcgagggcaa ccgcgacggc gagagccaca acaggtcgtg gaactgcggc
     6241 gtggaaggcc ccaccgacga ccccgagatc atcaagctgc ggtgccgtca gatgcgtaat
     6301 ttcctggcga cactgatggt ttcgcagggc actccgatga tcgcgcacgg cgacgagatg
     6361 ggccgcaccc agctgggcaa caacaacgtc tactgccagg actccaagct gtcgtggatg
     6421 gactggtcgc tgacggagcg taatgccgac ctgctcgagt tcagccggaa ggtgactgcg
     6481 ctgcgcaagc accatccggt gttccgccgc cgccgcttct tcgaggggac acctatccgc
     6541 agcggcgcgc agacccgcga catcgcctgg ctcacgcccg ccgggcagga gatgacaccg
     6601 gaagactggg gcagcgggtt cggaaagagc atcgcggtgt ttctcaacgg aagggcgatt
     6661 cccgaaccgg acgcccgtgg tttccccgtc gtcgacgact cgttcctgct gtgcttcaac
     6721 ggccatgacg aggatctgga tttcgttgtg ccggggggaa tctacggcag cgaatgggaa
     6781 gcgtgcctgg acaccgccga tccggagggc aacaccgacc tggtggtgcg ggtgggcgac
     6841 aagatcacgc tagaggcccg ctcggtgatc gtgttgcgca gagcagcgtg actggctgcg
     6901 cttcgtcccc cgcaagcggg aggtaccccc acctcgcgtc cgcggccgtc gtctccacct
     6961 accggctgca actgcgcggg ccggccagcg gtcaaccgtt cacgttcgcc gacgccgaga
     7021 acctgctcga ctacctcgac gatctgggtg tcacgcacgt gtatctgtcg ccgatcctca
     7081 ccgcggtcaa cggctccacc cacggctacg acgtcaccga cccgacaacg gttttcgacg
     7141 aactcggcgg tgcgaacggg ctggcgcggc tgtccgccgc ggcacgctcc cgagggatgg
     7201 ggttggtcgt cgacatcgtg cccaaccacc tcggcgtcga tgctcctgcg cagaacccgt
     7261 ggtggtggga tgtgctgcgg cacggccgcg attcggcata cgcgccatac ttcgacatcg
     7321 actggggctc ggacaacggc gtcgacggca agcttgccct gccggtgctg ggttccgacg
     7381 acgacgtcgc cgacctcacc ctcgacggcg acctgttgcg ccttggtgac ctcacgttcc
     7441 cgatcgcgcc gggcactggc gacgggacgg gaccgcaggt gcacgaccgc cagcactacc
     7501 ggctgatcgg atggcgccgc ggattgtgcg gctaccgaag gtttttcgcg atcacgtccc
     7561 tggccggact ccgacaggag gaccgtggcg ttttcgatgc cagtcacgcc gaggtacgcc
     7621 gt
//
LOCUS       DASWBF010000097         4458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_405435,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000097 DASWBF010000000
VERSION     DASWBF010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405435"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VF874_03365"
     CDS             <1..1344
                     /locus_tag="VF874_03365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9175158.1"
                     /translation="MDAFFASVEQLTRPTLRGRPVLVGGLGGRGVVAGASYEARAFGA
                     RSAMPMHQARRLVGVQAVVLPPRGVVYGLTSRRVFDTVRVVMPVLEQLSYDEAFGEPA
                     ELAGASAVDVERFCEVLRGRVRDDTGLVASVGAGSGKQIAKIASGLAKPDGIRVIRRD
                     EERLLLAALPVRRLSGIGPVAEEKLHRLGIQTIGQFADLTDAEAADVLGGTVGPALHR
                     LARGIDDRPVAERAEAKQISAESTFPTDLTTLAQLQQAVGPIGEHAHQRLMKDGRGAR
                     TVTVKLKRADMTTLTRTLTLPYATVDATTLIAAGRRLLLDPLEIGPIRLVGVGFSGLN
                     EVRQEPLFPDLDAWAEPTDTTSATAPTTVPSGWRIGDDVHHGQSGHGWVQGAGHGVVT
                     VRFETRASGPGPARTLPADDPALQRANPVDSLDWPEYVNALLDESAPAGDDVADG"
     gene            complement(1309..2199)
                     /locus_tag="VF874_03370"
     CDS             complement(1309..2199)
                     /locus_tag="VF874_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX9175159.1"
                     /translation="MSRVTVVTTGGTIASVADASGVLRPARTGAELLDGIDGIDVVDV
                     MAKDSSQLTAEDWDLISSAISTALDGSADGVVVTHGTDTMEETALWLELTYGGAAPVV
                     LTGAARSADAPDADGPANLRAAVALAANADSRGLGVVICFGGAVLAPLGTQKIAGGFD
                     GPQVRDRKERPFLGHVSAASAPRVDVVACYLGADAVALDACVAAGARGIVLEALGSGN
                     ASDAIVDAVRAHCAAGVTVAVSTRVPSGGVSARYGPGRQLVGAGAVEVRRLRPPQARV
                     LLMAALATGSSVGDVVARWG"
     gene            2290..2817
                     /gene="lspA"
                     /locus_tag="VF874_03375"
     CDS             2290..2817
                     /gene="lspA"
                     /locus_tag="VF874_03375"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX9175160.1"
                     /translation="MGDGVGARPRRRVGLLVTVAAVMLAVDIVSKVLAVRMLNPGQPV
                     SIIGDTVTWTLVRNSGAAFSMATGYTWILTLIATAVVIGIIWMGRRLVSPWWAIGLGM
                     ILGGALGNLIDRFFRWPGPLRGHVVDFLSIGWWPVFNVADACVVGGAALLVALSVFGY
                     DFDTVGRRTPDPVER"
     gene            2817..3746
                     /locus_tag="VF874_03380"
     CDS             2817..3746
                     /locus_tag="VF874_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9175161.1"
                     /translation="MADRSMPVPEGLAGMRVDAGVARLLGLSRTAAAALAEEGAVDID
                     GARAGKSDRLAAGAWLQVRLPDHPAPLKNRVEEVAGMTVLYADDDIVAVDKPAGVAAH
                     ASVGWTGPTVLGGLAAAGFRITTSGIHERQGIVHRLDVGTSGVMVVALSERAYTLLKR
                     AFKQRTVDKRYHALVQGHPDPSSGTIDAPIGRHRGHDWKFVVSEHGRHSITHYDTVEM
                     FPAASLLDVHLETGRTHQIRVHFAALHHPCVGDLTYGADPTLAKKLGLDHQWLHARSL
                     AFAHPADGRRIEITAPYPPDLQHALDVLRGHST"
     gene            3743..>4458
                     /gene="rarD"
                     /locus_tag="VF874_03385"
     CDS             3743..>4458
                     /gene="rarD"
                     /locus_tag="VF874_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX9175162.1"
                     /translation="MSTKDRSGLAYGVGAYLWWGLCPGFFPLLLPAGSLEVLAHRYVW
                     TALFLVLVLIVARRLGDLRRLSARTWLLLAAASVLIGINWGTYIFAVTHGHVVDAALG
                     YFINPLVSVALGVVVLRERLRRLQSVALVIAVAAVAMLTVQVGGPPVIALALATSFGV
                     YGLVKKVVDADPRVSVAVESLLALPFAGSYLVALQVLGNGHFVGFGVGHTTLLALAGP
                     VTAIPLLLFAAAAQRLPLITL"
BASE COUNT          609 a         1541 c         1577 g          731 t
ORIGIN      
        1 atggacgcgt tcttcgcgtc cgtcgagcag ctgacgcgcc cgacgctgcg ggggcgaccg
       61 gtgctggtgg gcggtctcgg cggccgcggg gtcgtcgccg gcgccagcta cgaggcccgc
      121 gcgttcggtg cacgctccgc gatgccgatg caccaggcac gccgcctcgt cggtgtccag
      181 gccgtcgtgc ttccgccgcg cggcgtcgtc tacgggctaa ccagccgccg cgtcttcgac
      241 acggttcgcg tagtgatgcc agtgctcgag cagctttcct acgacgaggc gttcggcgaa
      301 ccggccgaac tggccggcgc atcggcggtc gacgtcgaac ggttctgtga ggtattgcgg
      361 ggccgcgtcc gggacgacac cggcctggtc gcctccgtcg gcgccgggtc cggcaagcag
      421 atcgcgaaga tcgcgtccgg gttggccaag cctgacggca tccgcgtgat ccggcgcgac
      481 gaggagcgcc ttctgttggc ggcgctgccg gtgcgccggc tgtcggggat cggtcccgtt
      541 gccgaggaga agctgcaccg gctcgggatc cagaccatcg gccagttcgc cgacctaact
      601 gacgcggagg cggcagacgt tctcggcggg accgtcggcc cagcgctgca tcggctcgct
      661 cgtggcatcg acgaccgccc ggtggccgaa cgtgccgaag ccaagcagat aagcgccgag
      721 tcgacgttcc caaccgacct gacgacgttg gcgcagctgc agcaggcggt gggtccgatc
      781 ggcgagcacg cgcatcagcg gctgatgaag gacggccgcg gcgcgcgcac tgtgacggtc
      841 aagctgaagc gcgctgacat gaccaccctg acccgcacct tgacgctgcc gtacgccacc
      901 gtcgacgcca ccacgttgat cgcggcgggg cgtcggcttc tcctcgaccc gttggagatc
      961 ggcccgattc gcctcgtcgg agttggcttt tcggggttaa acgaggttcg tcaggagccg
     1021 ctgtttcctg acctcgacgc ctgggcggag cccaccgaca cgacgtcggc gacagcgccg
     1081 accacagtgc cgagtggctg gcgcatcggt gacgacgtgc accacgggca gtcgggccac
     1141 ggctgggtgc agggcgccgg gcacggcgtg gtgacggtgc ggttcgaaac ccgcgccagc
     1201 gggcccggcc ccgcgcgcac tcttcccgcc gacgaccccg cgctacagcg agccaacccc
     1261 gtcgacagcc tggactggcc cgagtacgtc aacgctctgc ttgacgagtc agccccagcg
     1321 ggcgacgacg tcgccgacgg atgaacccgt ggcgagcgcg gccatcagca acacccgcgc
     1381 ctgcggcggg cgcagccggc gcacctccac cgcgccggcc cccaccagtt gtcggcctgg
     1441 cccgtaccgg gcgctgaccc cgccggacgg cacgcgcgtc gacaccgcca ccgtgacacc
     1501 cgccgcgcag tgcgcgcgaa ccgcgtcgac gatcgcgtcg ctcgcgttgc ccgagcccag
     1561 cgcctccagc acgattccgc gcgcacccgc tgccacacac gcgtcaagtg cgaccgcgtc
     1621 cgcacccaga tagcacgcaa ccacgtcgac gcgcggcgcc gaggccgccg agacgtgtcc
     1681 cagaaacggc cgctctttgc gatctctgac ctgcgggccg tcgaaaccgc ccgcgatctt
     1741 ctgcgtgccc agcggcgcca gcaccgcgcc gccgaaacag atcaccacgc cgagtccgcg
     1801 gctgtcggcg ttggcggcca gcgcgaccgc ggcgcggaga ttcgccgggc cgtccgcgtc
     1861 gggggcgtcg gcgcttctcg ctgcgccggt cagcaccacc ggagccgccc cgccgtaggt
     1921 cagctccagc cacagcgccg tctcctccat ggtgtcggtg ccgtgagtga cgacgacgcc
     1981 gtccgcgctg ccgtcgagtg ccgttgagat cgccgacgag atcagatccc agtcctcggc
     2041 ggtcagctgc gaactgtcct tcgccatcac gtcgacgacg tcgatgccgt cgatgccgtc
     2101 gagcagctct gcgccggtgc gcgcgggacg caacacgccg ctggcatcgg ccaccgaagc
     2161 gatggttccg cccgtggtca cgaccgtgac gcggctcatg ggcgaatcat gcatcacggc
     2221 gtcgtgctgt ggtcaatggc cttttgggat gatgggcaag tgagcgacga atcgaggccc
     2281 gcatcggcca tgggcgatgg cgtcggcgct cggccgcggc gccgggtcgg gctgctggtc
     2341 acggtggccg cggtgatgct ggcggttgac atcgtcagca aggttctcgc ggtgcgcatg
     2401 ctcaatcccg ggcagccggt gtcgatcatc ggcgacacgg tgacgtggac tctggtccgt
     2461 aactccgggg cggccttttc gatggccacc ggctacacct ggatcctcac gctgatcgcc
     2521 accgcagtcg tcatcggcat catctggatg ggccggcggc tggtgtcccc gtggtgggcg
     2581 atcggactcg gcatgatcct gggcggggcc ctgggcaacc tcatcgaccg gttcttccgc
     2641 tggcctgggc cgctgcgcgg gcatgtcgtc gatttcctgt cgatcgggtg gtggccggtg
     2701 ttcaacgtcg ccgacgcgtg tgtggtcggc ggtgccgcgc tgctggtggc gctgtcggtg
     2761 ttcggctacg acttcgacac cgtcgggagg cggacgccgg accccgttga gcgctgatgg
     2821 ctgatcggtc gatgccggtc ccagagggac tggccggaat gcgggtcgac gccggcgtgg
     2881 cccggctgct gggactgtcc cgtaccgccg cggcggcact ggcggaggag ggggccgtcg
     2941 acatcgacgg cgcccgcgcc ggtaagtcag accgcctggc cgcgggcgcc tggcttcagg
     3001 tgcggctgcc cgatcatcct gcgcccctga agaaccgggt cgaagaagtg gctggcatga
     3061 cggtgctcta cgccgacgac gacatcgtcg cggtggacaa acccgcgggc gtcgccgcgc
     3121 acgcctcggt gggctggacc ggcccgaccg tgctcggcgg tctggctgcg gccggtttcc
     3181 ggatcaccac gtcgggaatt cacgaacgac agggcatcgt gcatcgtctc gacgtgggca
     3241 cttcgggggt gatggtggtc gcgttgtccg agcgtgccta cacattgctc aagcgcgcgt
     3301 tcaagcagcg caccgtcgac aagcgttatc acgctctggt acaaggtcat ccggacccgt
     3361 caagcggcac catcgacgcg ccgatcggcc ggcaccgtgg ccacgactgg aagttcgtgg
     3421 tgtccgagca tggccgtcac agcatcaccc attacgatac tgtggaaatg tttccggccg
     3481 cgagcctgct agacgttcac ctggaaaccg gtcgtacaca ccagattcgg gtacacttcg
     3541 cggcgctgca ccatccctgc gtcggcgacc tgacctacgg tgccgacccg acactggcga
     3601 aaaagcttgg gctggaccat caatggctgc acgcgcggtc cctggccttc gcgcatccag
     3661 ccgacggccg tcgcatcgag atcaccgcgc cgtacccgcc cgacctgcag cacgcgctcg
     3721 acgtgctgcg cggtcacagc acgtgagcac gaaggaccgc tccgggctgg cctacggggt
     3781 cggcgcgtat ttgtggtggg gattgtgccc cggctttttt ccgctgctgc tgcccgccgg
     3841 gtcgctggag gtgctggcgc accgctacgt ctggacggcg ctgttcctgg tcctcgtgct
     3901 gatcgttgcg cgccggctcg gcgacctgcg ccggctcagc gctcgcacct ggctgctgct
     3961 ggcggcggcc tcggtactaa tcgggatcaa ttggggcaca tacattttcg cggtaacaca
     4021 cggccatgtg gtggatgccg cgctcgggta cttcatcaac ccgctggtgt cggtggcgct
     4081 gggtgtggtt gttctgcggg agcgtctgcg gcggctgcag tcggtggcgc tggtgatcgc
     4141 ggtggcggcg gtggcgatgc tgacggtcca ggtgggcggc ccgccggtga tcgcgctggc
     4201 gctggccacg tcgttcgggg tgtacggcct ggtgaagaag gtggtcgacg ccgatccgcg
     4261 ggtcagcgtg gcggtcgagt cgctgctggc gctgccgttc gccgggagct acctggtcgc
     4321 gctgcaggtg ctcggcaacg gtcacttcgt cggattcggc gtcggacaca ccacgctgct
     4381 ggcgctcgcc ggcccggtga ccgcgattcc gctgctgctg ttcgccgcgg cggcgcagcg
     4441 cctgccgctg atcaccct
//
LOCUS       DASWBF010000098         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_337899,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000098 DASWBF010000000
VERSION     DASWBF010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337899"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..495
                     /locus_tag="VF874_03390"
     CDS             19..495
                     /locus_tag="VF874_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX9175163.1"
                     /translation="MTDERYVVTRTIAATPGEIFAILSDPSRHRDTEPGDWVRDAIDT
                     EPIIGTGQIFAVNMFLERLGGPYVMHNLVTAFEQDRTIVWSPGRLDDAGNHQPGGWTW
                     RYDLAPNGTGTDVTLTYDWSGTPQQFRDSVRRMPPFGVEFIEDSLDALGRSVAPTG"
     gene            625..1176
                     /locus_tag="VF874_03395"
     CDS             625..1176
                     /locus_tag="VF874_03395"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175164.1"
                     /translation="MILEALASFTEPVDVIDYQQHMPEVPAPEALPPIEVQLADYANE
                     LDGFWVQPRAVSTRYIDPPPLLAADLPDAPAPVSRLWLRVGGDVPTDPLTASALLAYI
                     SDWSILDPVLYVTRRPPVASSIASLDHAMWFHRPPDFSDWLLYDQRSPSGIGARGLSN
                     GAIYNRAGQLVCTVVQEGYLGRS"
     gene            complement(1179..2045)
                     /locus_tag="VF874_03400"
     CDS             complement(1179..2045)
                     /locus_tag="VF874_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9175165.1"
                     /translation="MPIDPNAVGAKTEPQLFTWTDRDTLLYALGVGAGVDDLAFTTEN
                     SHDIDQQVLPTYAVIACLPFAAAAKIGTFNFAMLLHGSQAIRLFAPLPPAGTLSVGAE
                     VADIQDKGEGKNAIVVLKATGTDPETSQVVAETLTTAVIRGEGGFGGQPGQRPVPPEI
                     PEREPDARVALPTREDQALLYRLSGDRNPLHSDPWFAREMAGFPKPILHGLCTYGVAG
                     RALVAELGGGDATKVRAVGARFTSPVFPGETLTTSIWRTEPGHAVFRTEASNPDGSNT
                     RLVLEDGAAEYS"
     gene            complement(2064..2231)
                     /locus_tag="VF874_03405"
     CDS             complement(2064..2231)
                     /locus_tag="VF874_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175166.1"
                     /translation="MATSGETVRSWPSRAKPEVLCAKLTTNMSHQQWNEWVLTEIDYM
                     KVCPDLPITPD"
     gene            complement(2379..3944)
                     /locus_tag="VF874_03410"
     CDS             complement(2379..3944)
                     /locus_tag="VF874_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9175167.1"
                     /translation="MESGKSGTTGGIGSWAARRAFLSGDRIAIIDGDNRITYAEFDRR
                     TDQFARALRELGVRQGDRVAVLMVNSAALLETMFATAKLGAVFVPINFRLAPPEVSYL
                     LADSGAHVFVWSGQLFPLARAALAREGVQVRARVVVGGESSDGEADFEQALASGEPRA
                     LGTNAAGSDLCCLMYTSGTTGRPKGAMLTHDNLLWNAINTVMGHRLREGDRTVTVAPM
                     FHIGGLGVHTLPLLYVGGTSAILPAFDPEKTLATMARERVTVQFLVPAMWSALMTVPG
                     FETYDLSALELAVTAGAPCPLPVLEYFRGKGVPFQEGFGLTETAPTVSILDADHVKAK
                     AGSIGRALFHVETRIVDENDRDVHTGEVGELVVRGPNVFAGYWGLPEATAEAFRGGWF
                     HTGDLGRMDAEGFITLVDRKKDMIITGGENVYPIEVEQVLYRHPAVREVAVVGVPHTK
                     WCETPIAVVALKDGAQVTGDELIGYARERLAHFKCPTRVEYVPELPRTATDKVLKTTL
                     RKEYAGQETTVQR"
BASE COUNT          675 a         1400 c         1286 g          694 t
ORIGIN      
        1 cggacaagac tccaactcat gaccgatgag cgttacgtcg tgacccgcac catcgccgcc
       61 acaccgggcg agatcttcgc gatcctttcc gacccgtcgc ggcatcggga caccgaaccg
      121 ggcgactggg tgcgcgacgc gatcgacacc gaaccgatca tcggcaccgg gcagatcttc
      181 gcggtgaaca tgtttctgga acggctcggc gggccctacg tcatgcacaa cctggtgacc
      241 gcattcgaac aggaccggac gatcgtgtgg tctcccgggc gactcgacga cgcgggcaac
      301 caccaacccg gcggctggac gtggcgctac gaccttgctc cgaacgggac gggcaccgac
      361 gtcaccctca cctacgactg gagcgggaca ccccagcagt tccgcgacag tgtccgccgc
      421 atgccaccgt tcggagtgga gttcatcgag gactcgctcg acgcgctcgg ccgctccgtc
      481 gcaccgaccg gttagccgtt ctcgacgacg atccgactgc ggccgggtaa cccgcagacg
      541 ccgctgcggt acgccgtcac caacttgcgc gacggcggca cattctctgc tcgcgccgtc
      601 tcggtcaccc agggtgacga tcaagtgatt ctcgaagcgc tggcgtcgtt caccgaaccc
      661 gtcgacgtca tcgactatca gcagcacatg cccgaggtac cggcgccgga agcgttgccg
      721 cccatagaag ttcagctcgc cgactatgcg aacgaactcg acgggttctg ggtgcagccg
      781 cgcgcggtca gcacgcgcta catcgatcca ccgccactgc tggcggccga cctgcctgac
      841 gcgccggctc cggtgagccg actgtggcta cgcgttggcg gcgatgtgcc gaccgaccca
      901 ttgaccgcca gcgctctgct cgcctacatc agcgactggt cgattctcga cccggtgctc
      961 tacgtgactc gccggccgcc ggtggccagc agcatcgcct ccctcgacca cgccatgtgg
     1021 ttccaccggc cgcccgactt ctcggattgg ctgctctacg accagcgctc accgagcgga
     1081 atcggcgccc gcggcctgtc caacggtgcg atctacaacc gggcggggca gttggtgtgc
     1141 acggtggtgc aagagggcta cctgggccgc agctgacgtt agctgtactc ggcggcgccg
     1201 tcctccagca ccagccgggt attggacccg tccgggttcg acgcctccgt gcggaacacc
     1261 gcatgaccgg gttccgtccg ccagatcgag gtcgtcaacg tctcgccagg gaacaccgga
     1321 gaggtgaacc gcgcgccgac ggccctgacc ttcgtggcgt ctccgccacc gagttcggcg
     1381 accaacgccc ggcccgcgac gccatacgtg cacagcccgt gcaggatcgg cttcgggaac
     1441 ccggccatct ctcgtgcgaa ccacgggtcg ctgtgcagcg gattgcgatc gcccgacagc
     1501 cggtagagca gcgcctggtc ctcgcgcgtc ggcagcgcga cccgcgcgtc gggctcacgt
     1561 tccggaatct ccggcggcac cggacgctga ccgggctgcc cgccgaagcc gccctcgccg
     1621 cggatgaccg cggtcgtcag cgtctcggcg accacctgcg acgtctccgg gtcggtgcct
     1681 gtggccttga gcacgacgat cgcgttcttg ccctcgccct tgtcctgaat gtcggcgact
     1741 tcggcgccga cgctcaacgt cccggccggt ggcagcggcg cgaaaagccg gatcgcctgt
     1801 gatccgtgca gcagcatcgc gaagttgaag gtgccgatct tggcggctgc ggcgaacggc
     1861 aggcaggcga tcaccgcgta cgtcggcaat acctgttggt cgatgtcgtg actgttctcg
     1921 gtggtgaagg cgaggtcgtc gacaccggcg ccgacgccga gcgcatagag caacgtgtcg
     1981 cggtcagtcc aggtgaagag ctgcggctcg gttttcgcgc caacggcgtt cgggtcaatc
     2041 ggcattgcag ctccttcgag tcgctagtcg ggtgtgatcg gcagatccgg gcaaaccttc
     2101 atgtagtcga tctccgtcaa cacccattcg ttccactgct ggtggctcat gttggtggtc
     2161 aacttggcgc acaggacttc tggtttcgca cgcgacggcc atgatctcac cgtctcgcca
     2221 ctcgtggcca cgatgcgctg accatccgca tcagatcgct gatcgcatct ccgtactgcg
     2281 tcgaaggcaa tgccgcgacg ccagttggcg tataagtaga ccgtcctagg tggctgtcga
     2341 tgctgacgcc taaccgcgag ccggccagct cggtgacttc accgctgcac ggttgtctcc
     2401 tggccggcgt actccttacg cagcgtcgtc ttgagcacct tgtcggtggc ggtgcggggc
     2461 agttccggca cgtactcgac gcgggtgggg cacttgaagt gggccaggcg ctcgcgggcg
     2521 taaccgatca gttcgtcgcc ggtcacctgc gcgccgtcct tgagagctac gacggcgatg
     2581 ggtgtctcac accatttggt gtgcggcaca cccacgacag cgacttcacg gactgccggg
     2641 tggcggtaga gcacctgctc aacctcgatc gggtagacgt tctccccgcc ggtgatgatc
     2701 atgtccttct tgcggtcgac cagggtgatg aagccctcgg catccatgcg gcccaggtct
     2761 ccggtgtgga accagccgcc gcgaaacgcc tcggcggtcg cctcgggcaa cccccagtag
     2821 ccggcgaaga cgtttgggcc gcgaacgacg agctcgccca cctcgccggt gtgcacatcg
     2881 cggtcgttct cgtcgacgat gcgagtctcc acgtggaaca aggctcgccc gatcgaaccc
     2941 gccttcgcct tgacatgatc ggcgtcgaga atcgaaaccg taggagccgt ctcggtcagg
     3001 ccgaatccct cctgaaacgg cactcccttt ccgcggaagt attcgagcac cggcaacggg
     3061 cacggcgcgc cagcggtcac agccagctcg agcgcagaca ggtcgtaggt ttcaaagccc
     3121 ggaaccgtca tcagcgcgga ccacatcgcc ggtaccagga actggacggt gacgcgctcc
     3181 cgcgccatcg tggcgagcgt cttctccgga tcgaacgccg gcaggatcgc gcttgtacca
     3241 ccgacataca gcagcggaag ggtgtgcacc ccaagccccc cgatgtggaa catcggcgcg
     3301 accgtgactg tccggtcgcc ctcacggagc cggtgcccca tgactgtgtt gatcgcattc
     3361 cacaagaggt tgtcgtgggt gagcatcgcc cctttggggc gcccggtagt gcccgacgtg
     3421 tacatgaggc aacacaggtc gcttccggcc gcgttggtgc cgagcgcacg cggctcgccg
     3481 ctcgcgagcg cctgttcgaa gtctgcctcg ccgtcggagg actcgccgcc gaccaccact
     3541 cgggcgcgta cctgcacgcc ctcgcgggca agagcggcgc gggcaagcgg gaacagctga
     3601 cccgaccaca cgaaaacatg ggcgcctgag tcggccagca ggtaggacac ttccggcggg
     3661 gccagccgga agttgatggg cacgaagacc gcccccagct tcgcggtggc gaacatcgtc
     3721 tctagcaagg cggcgctgtt gaccatcaaa acggccaccc ggtcgccctg ccgcacaccc
     3781 aattcgcgca gtgcgcgggc gaattggtct gtgcgccggt cgaactcggc gtaggtgatg
     3841 cgattgtctc cgtcgataat cgcgatacgg tctccggaca ggaaggcccg ccgggccgcc
     3901 cagctgccta tcccaccggt ggtcccgctt ttaccgcttt ccatttcggc gacctttccg
     3961 cggcggcaat ttcactcatg attgcgcatc tcgcgacgaa gcactcctga acgccgagca
     4021 tccagcacga gcatggcggg aaccgctgtg tcggt
//
LOCUS       DASWBF010000099         5638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_269923,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000099 DASWBF010000000
VERSION     DASWBF010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269923"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..2050)
                     /gene="asnB"
                     /locus_tag="VF874_03415"
     CDS             complement(83..2050)
                     /gene="asnB"
                     /locus_tag="VF874_03415"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX9175168.1"
                     /translation="MCGLLALVRDPAATPAGTGAVVDQVAESAHLMRHRGPDEPGTWA
                     SGAAAPATSGGDEAVFGFNRLSIIDIEHSHQPLRWGPADAPGRYVLVFNGEIYNYLEL
                     RAQLSAEQGAVFATDGDGEAIIAAYHYWGADALARLRGMFAFALWDTERHELFCARDP
                     FGIKPLFMATGPGGTAVASEKKCLLDIAGLLGLDLAIDERAVQHYTVLQYVPEPETLH
                     RGIRRLESGCSAVIRPGQPPQITRYFVPRFAATPFNNGSEQARYDEITAALEDSVAKH
                     MRADVTVGAFLSGGIDSTAIAALAIRHNPRLITFTTGFEREGFSEVDVAVASAEAIGA
                     RHVSKVVSPAEFVDALPEIVWYLDEPVADPALVPLFFIAREARKHVKVVLSGEGADEL
                     FGGYTIYREPLSLKPFDYLPGRLRRSMRKVSAPLPEGMRGKSLLHRGSQTLEERYYGN
                     ARSFSDPQLQAVLKIFRPEWTHTDVTAGVYEQSQDWDPVARMQHIDLFTWLRGDILVK
                     ADKMTMANSLELRVPFLDPEVFAVASRLPLEQKITRTTTKYALRRALEPIVPAHVLNR
                     PKLGFPVPIRHWLQAGELLDWAHEMLRATQADELIDVAAVRRMLDEHLAGTSDHSRRL
                     WTVLIFMLWHAIFVERSIVPQISEPQYPVEI"
     gene            2136..3155
                     /locus_tag="VF874_03420"
     CDS             2136..3155
                     /locus_tag="VF874_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9175169.1"
                     /translation="MKRLRLLGLALTLGVAALTLTGCNAYDALKLGWPEGITPEAHAN
                     LKLWIGSIIAAFVVGIIVWGLTFWTAAFHRHKKTDTDLPRQFGYNMPLELVLTVTPFL
                     IIAVLFYFTVVVQERMLHKEPNPEVVVDVTAFQWNWKFGYQSVNFKDGTYNVNLVDNE
                     RKLAEVSKPEGIDSHGEEIVGAIRGMNPEDRSYLNFDKVETLGTSNEIPVLVLPAHKR
                     IEFQLASADVVHAFWVIDFLFKRDVFPDPEANHTDNKFQVTEIEKTGAFVGRCAELCG
                     TYHSMMNFEVRVVEPNVFKAYLDARIAGKTNAEALQIIGLPPAAVTTQPFDTRRGEQS
                     LSAQK"
     gene            3165..3584
                     /locus_tag="VF874_03425"
     CDS             3165..3584
                     /locus_tag="VF874_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEX9175170.1"
                     /translation="MHIEARLFEILTAFFTVATVVYAFLTAYFFPGGVEWAGVTALVM
                     TTGLTLIIGTFFRFVARRLDTRPEDYEDAEIADGAGELGFYAPHSWWPVMVALAASVT
                     AVGVALWLPWLIVTGAVLVLSAAAGLVFEYYIGPEKH"
     gene            3761..4600
                     /locus_tag="VF874_03430"
     CDS             3761..4600
                     /locus_tag="VF874_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HEX9175171.1"
                     /translation="MSGPNPGDEPVHGVPTQDVSADTGLSQAYSAPESEQFTSGPYVP
                     ADPALYDYDTYDPAAEHDDEPPTPRWPWVVGVAAILAAIALVASVSLLVFPSLTSNEA
                     RPTTTTPPPPVQDEITETTPPPPPPPTSEPPPPPPPTETVTVTSEPPPPPPTSEAPPP
                     PPAPSPSAPATTSAAPPPPPPPTTTTTTTGPRQVTYTVTGTKAPGDIISITFTDASGR
                     SRTQRNVYIPWSFTLTPISNSEIGSVQASSFFQVSKLNCSITASDGTVLSQRSDNSPV
                     TSC"
     gene            4600..5265
                     /locus_tag="VF874_03435"
     CDS             4600..5265
                     /locus_tag="VF874_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2561 family protein"
                     /protein_id="HEX9175172.1"
                     /translation="MDRSETVDRALLGACAVVWLAVIGVSVAAVVALVELGSGRAGEP
                     APNSGTPWGLYVVIGISAAIILASVPLLLRARRAAQQEPPRRPPSTAPPSTAPRSTAP
                     PSTAPRRPAAPAVEVPIEKLRVFGSVADSAGEQPPGGKRPPPPSPYGGTLSDDLVEAL
                     WLRFTLSMTAAMGVATLAVATATYFMAVKHDGWAWAALTVAGIVTALMPLIPWRQLRP
                     LRA"
     gene            complement(5270..>5638)
                     /locus_tag="VF874_03440"
     CDS             complement(5270..>5638)
                     /locus_tag="VF874_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX9175173.1"
                     /translation="IAYRWAIALQRSDREVLEHGVETGIIKRLPHGAYIELHQPLGPV
                     DDHGHPIPLEYQGAPVPKRMNKLGSGGAPGLGSLLTADPPAEQQALTESAHAQEQRAI
                     TALRERQDSDGNGSSPDGQH"
BASE COUNT          927 a         1950 c         1854 g          907 t
ORIGIN      
        1 ctcggtgtcg cgcatgcggc aaatcctgcc gctgaccgcc cgtcaaaccc aagccggacc
       61 ccacacagaa gccggaagag ccctagattt caaccgggta ctgcggctcg ctgatctgcg
      121 gcacaatgct gcgctcgacg aagatcgcgt gccagagcat gaagatcagc accgtccaga
      181 gccgacggct gtgatcgctg gtgccggcga ggtgttcgtc gagcatccgg cgcaccgcag
      241 cgacgtcgat cagttcgtcg gcctgcgtgg cgcgcagcat ctcgtgtgcc cagtcgagca
      301 gctcgcctgc ctgcagccag tgccggatcg gaacggggaa accgagtttc ggccggttca
      361 gcacatgtgc aggcacgatc ggctccaacg cccgccgcag cgcgtacttg gtggtggtgc
      421 gggtgatctt ctgctccagc ggcagccgtg acgccaccgc gaacacctcg ggatccagga
      481 acggcacgcg cagctccagc gagttggcca tcgtcatctt atcggccttc accaaaatgt
      541 cgccacgcag ccacgtgaac aggtcgatgt gctgcatgcg cgccaccgga tcccagtctt
      601 gagactgttc gtacactcct gcggtgacgt cggtgtgcgt ccattccgga cggaagatct
      661 tcagcaccgc ctgcagctgc ggatcggaaa agctccgggc gttgccatag tagcgctcct
      721 ccagcgtctg tgacccgcgg tgcagcaggc tttttccacg cattccctcc gggagcgggg
      781 ccgagacctt gcgcatcgac cggcgcagcc gacccggaag gtagtcgaaa ggcttcagcg
      841 acaacggttc ccggtagatc gtgtacccgc cgaacagttc gtcggcgccc tcgccggaca
      901 gcaccacctt gacatgcttg cgtgcctcgc gggcgatgaa gaacagcggc accaacgccg
      961 ggtcggcgac gggctcgtcg agataccaga cgatctcggg cagcgcgtcg acgaactccg
     1021 cggggctgac caccttggac acgtgccgcg cgccgattgc ctccgccgac gcgacggcga
     1081 cgtcgacctc tgaaaaccct tcccgctcaa agccggtggt gaacgtgatc agccgcgggt
     1141 tgtgccggat ggcgagcgcg gcgatcgcgg tcgaatcgat gccgccagac aggaacgctc
     1201 ccactgtgac gtcggcgcgc atgtgcttgg ccaccgagtc ctcgagcgcg gcggtgattt
     1261 cgtcgtagcg ggcctgctca ctgccgttgt tgaacggcgt ggcggcgaac cgcggcacga
     1321 aatagcgggt gatctgcggc ggctgtccgg ggcggatgac cgctgagcag ccggactcca
     1381 gccgccggat gccgcggtgc agggtctccg gctcggggac gtactgcaga accgtgtagt
     1441 gctgcacggc ccgttcgtcg atcgcgaggt cgaggcccag tagtccggcg atatcgagca
     1501 gacacttctt ttcgcttgcc accgcggtgc cgcccgggcc ggtcgccatg aacagcggtt
     1561 tgatgccgaa cgggtcacgc gcgcagaaca gttcgtggcg ctccgtgtcc cacagtgcga
     1621 acgcgaacat gccgcgcaac cgcgcgagcg cgtcggcacc ccagtagtga taggccgcga
     1681 tgatcgcctc gccgtcgccg tccgtggcga acaccgcgcc ctgctcggcg gaaagctgcg
     1741 cccgcagctc caggtagttg tagatctcgc cgttgaacac cagcacgtag cggccgggcg
     1801 cgtccgcggg cccccaccgc agcggctgat gagagtgctc gatgtcgatg atcgacagcc
     1861 ggttgaagcc gaacactgcc tcgtcgccgc cagatgtcgc gggcgcggcg gctccggagg
     1921 cccaggtgcc cggctcgtcg ggaccgcggt gccgcatcag gtgcgctgac tcggcgacct
     1981 ggtcgaccac agcgcccgtg cctgcgggag tggccgcagg gtcgcgcacg agggccagca
     2041 gtccgcacat cgaggccaag tatgccgcac gcgccgggtg gtctacgctg cgtagtagca
     2101 actgcgactg tccccgttga agcacaggag gcgccttgaa acggctgcgg cttctcggct
     2161 tagctctcac tttgggtgtg gcggcgctca cgctcaccgg gtgcaacgcc tacgatgcac
     2221 tgaaactggg ctggccggag gggatcacac cggaggcgca cgccaacctc aagctgtgga
     2281 tcggctcgat catcgccgct ttcgtcgtcg gcatcatcgt gtggggcctg acgttctgga
     2341 cggcggcgtt tcaccggcac aagaagaccg acaccgacct gccccggcag ttcggctaca
     2401 acatgccgct cgagctggtc ctgacggtca cgccgttcct gatcatcgcg gtgctcttct
     2461 acttcacggt cgtcgtgcag gaacggatgc ttcacaagga gccgaatccc gaggtcgtcg
     2521 tcgacgtcac cgcgttccag tggaactgga agttcggcta ccagagcgtg aacttcaagg
     2581 atggcaccta caacgtcaac ctcgtcgaca acgagcgcaa gctggccgag gtgtccaagc
     2641 cagaaggcat cgacagccac ggcgaggaga ttgtcggcgc gatccgcggc atgaaccccg
     2701 aggaccgctc ctacctcaac ttcgacaagg tcgagacgct gggcaccagc aacgagatcc
     2761 cggtgctggt gctgccggcg cacaagcgca tcgagttcca gctggcctcc gccgacgtcg
     2821 tccacgcgtt ctgggtcatc gacttcctat tcaagcgcga cgtgttcccc gacccggagg
     2881 ccaaccacac cgacaacaag ttccaggtca ccgagatcga gaagaccggc gcgttcgtcg
     2941 ggcggtgcgc cgagctctgc ggcacctatc actcgatgat gaacttcgaa gtgcgcgtgg
     3001 tggaacccaa cgtcttcaag gcctacctcg acgcgcggat tgccggaaag acgaacgccg
     3061 aggcgctgca gatcatcggt ctgccaccgg ccgccgtcac cacccaaccg ttcgacacgc
     3121 gccgcggcga acagtccctg tccgcgcaga agtaaggaca cccgatgcac atcgaagccc
     3181 ggctgttcga gatcctgacg gcgttcttca ccgtggcgac cgtggtgtac gcgtttctga
     3241 ccgcgtattt ctttcccggc ggagtcgagt gggcaggtgt cacggcgctg gtgatgacca
     3301 ccgggctgac actgatcatc ggcacgttct tccggttcgt cgcacgccgg ctcgacaccc
     3361 gtccggagga ctacgaggat gccgagatcg ccgacggcgc gggggagttg ggcttctatg
     3421 cgccgcatag ctggtggccg gtcatggtcg cgctggccgc gtcggtcacc gcggtcgggg
     3481 tagcgctgtg gctgccgtgg ctgatcgtca ccggtgcggt gttggtgctg agcgccgcgg
     3541 cgggcctggt tttcgagtac tacatcgggc ccgagaagca ctaagccccg gacgcgagga
     3601 ggacaagaat ccccgccccg aaggtcacaa tccggcagtc agttgccttg tcgacgccgt
     3661 cggtggattc gatcgatccg cacgtttcgg tagtgttgcc gagggcaact tcgcgtgccc
     3721 gcacaccctg tagagcggta gttgagaagg aattgcagcg atgagcgggc cgaaccccgg
     3781 tgacgagccg gtccacggcg tgcccaccca ggacgtgagc gccgacaccg ggttgtcgca
     3841 ggcgtactcc gccccggagt ccgagcagtt caccagcggc ccgtatgtcc cggccgatcc
     3901 cgcgctctac gactacgaca catacgaccc ggcggccgaa cacgacgacg agccccccac
     3961 cccgcgctgg ccgtgggtag tcggtgtagc ggcgatcttg gcggccatcg cgctggtggc
     4021 gtcggtgtcg ctgctggtgt tcccgagcct caccagcaat gaggcccgcc cgaccacgac
     4081 gacgcccccg ccgccggtgc aagacgagat caccgagacg acgccgccac cgccgccgcc
     4141 gccgaccagc gagccgccgc cccctccgcc gccgaccgag acggtcacgg tgacgtcgga
     4201 gcccccgcct ccaccgccta ccagtgaggc tccgcctccg ccgccggcgc cgagcccgtc
     4261 ggccccggcg accacaagcg ccgcaccacc accaccaccg ccgccgacca ccacgaccac
     4321 gacgaccggc ccccggcagg tgacgtacac ggtgaccggc acgaaggctc ccggtgacat
     4381 catctcgatc acgttcaccg acgcgtccgg acgcagccgt acgcagcgca acgtctacat
     4441 cccgtggtcg ttcacgttga cgccgatctc gaactcggag atcgggtcgg tgcaggcgtc
     4501 cagtttcttc caggtgagca agctgaactg ctcgatcacc gcgagcgacg gcacggtgct
     4561 gtcccagcgt tccgacaatt caccggtgac cagctgctga tggaccgttc cgaaaccgtc
     4621 gaccgcgcat tgctgggcgc ctgcgccgtg gtctggctcg ccgtgatcgg ggtaagcgtc
     4681 gcggcggtcg tcgcgctggt ggagttgggt tccggccggg ccggcgagcc ggcccccaac
     4741 tccggcacgc cgtgggggct ttacgtggtg atcggcatct cggcggcgat catcctcgcg
     4801 tcggttccgc tgctgctgcg cgcgcgccgg gcggctcagc aggagccgcc gcggcgcccg
     4861 ccaagcacag caccgccaag cacagcaccg cgaagcacag caccgccaag cacagcaccg
     4921 cgaaggcccg ccgcgcccgc cgtcgaggtg cccatcgaaa agctgcgcgt gttcggttcg
     4981 gtggccgact ccgcgggcga gcagcccccg ggcgggaagc ggccaccgcc gccgtcaccg
     5041 tatggcggca cgctgtccga cgatctggtc gaggcgttgt ggctacgctt cacgctgtcg
     5101 atgacagccg cgatgggcgt ggcgacactg gcagtcgcca ccgccaccta tttcatggcc
     5161 gtcaaacatg acggctgggc gtgggccgcg ctaacggtgg ccggaatcgt caccgcgctg
     5221 atgccgctca tcccgtggcg gcagctgcgg cccttgcgcg cttagtcgct cagtgctggc
     5281 cgtccggcga cgatccgttc ccgtcgctgt cctgccgctc gcgcagcgcg gtgatcgcac
     5341 gctgctcctg cgcatgtgcg gactcggtga gcgcctgctg ctcggccggt ggatccgcgg
     5401 tcagcaggct gcccaacccg ggcgcgccgc ccgagccgag cttgttcatc cgcttgggca
     5461 ccggagcgcc ctggtactcc agcggaatcg ggtggccgtg gtcgtcgacc ggtcccagcg
     5521 gctggtgcag ctcgatgtag gcgccgtgcg gcagccgctt gatgatgccg gtctcgacgc
     5581 cgtgctcgag cacctcacgg tcgctgcgct gcagcgcgat tgcccaccgg tacgcgat
//
LOCUS       DASWBF010000100        10688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_732866,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000100 DASWBF010000000
VERSION     DASWBF010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732866"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VF874_03445"
     CDS             <1..464
                     /locus_tag="VF874_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX9175174.1"
                     /translation="KPTDTGITNGQISRVYGRQASEYVIQRGMSQIGVPYSWGGGTAA
                     GKSIGQGDATVGFDCSGLVLYSFAGVGIKLPHYSGAQYDMGRKIPAAQMRRGDVIFYG
                     PGGSQHVTIYLGKDASGVGQMLEAQQTGVPVKVSPVRTSGMTPYVVRYIEY"
     gene            483..1160
                     /gene="ripB"
                     /locus_tag="VF874_03450"
     CDS             483..1160
                     /gene="ripB"
                     /locus_tag="VF874_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family peptidoglycan endopeptidase
                     RipB"
                     /protein_id="HEX9175175.1"
                     /translation="MRPTRLLRTVCVVLAPLLIAFATPAAAEPDGWDPTLPQIISAGA
                     PGDPLAIANASLQASAQAAQTTMDLGRKFLGSLGLFPNSTAAPGRVYGAQAIEYVIRR
                     GGSQIGVPYSWGGGSLDGPTTGIDSGAGTVGFDCSGLMRYAFAGVGVLIPRFSGDQYT
                     AGRHIPPSQAKRGDLIFYGPGGGQHVTMYLGNGQMLEASSLAGKVTVSPVRTAGMTPY
                     LTRMIES"
     gene            1267..2421
                     /locus_tag="VF874_03455"
     CDS             1267..2421
                     /locus_tag="VF874_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9175176.1"
                     /translation="MTSPGGPPQGAGTFSGPGGAPGYPGAQTAPAAAPPPERNGGLAS
                     EVHTLERAVYEVKRIIVGQDQLVERMLVGLLAKGHVLLEGVPGVAKTLAVETFARVVG
                     GTFARIQFTPDLVPTDIVGTRIYRQGKEEFDIELGPVVVNFLLADEINRAPAKVQSAL
                     LEVMQERRISIGGKDFSMPNPFLVMATQNPIEQEGVYALPEAQRDRFLFKLNIDYPSP
                     EEEREIIYRMGVKPPEPKQILAPGDLLRLQDVASNIFVHHALVDYVVRIVTATRRPDQ
                     FGMPDARAWIAYGASPRASLGIIAAARALALVRGRDYVIPQDVIEVIPDVLRHRLVLT
                     YDALADEISPETVITRIMQTVALPQVNAVPQQGHSVHPVVPAAAAAASGR"
     gene            2418..3365
                     /locus_tag="VF874_03460"
     CDS             2418..3365
                     /locus_tag="VF874_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9175177.1"
                     /translation="MSPSAGRTVDLPSLQRGQIRDPELSAALRKLELTVRRKLDGVLH
                     GDHLGLIPGPGSEPGESRLYQPGDDVRRMDWSVTARTNHPHVRQMIADRELETWLVVD
                     MSASLDFGTTGCEKRDLAVAAAAAIAFLNSGGGNRLGALIANGQDIIRVPALSGRMHE
                     QTLLRAIATSPKAPVGVRGDLAAAIDDLRRPERRRGMAVIISDFLGPINWMRPLRAIA
                     GRHEVLGIEVLDPRDVELPDVGDVILQDAETGVTREFTIDAKLRDDFAKAAAVHRAEV
                     ARTLRRCDAPLLSLRTDRDWIADVVRFVSSRRRGAMAGR"
     gene            3515..4522
                     /locus_tag="VF874_03465"
     CDS             3515..4522
                     /locus_tag="VF874_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9175178.1"
                     /translation="MNVPLLGPMTLTGFAHTWWFLFLLVVVGLVVAYVLAQLARQRRV
                     LRFANMELLESVAPQRPNRWRHLPAILLVASLVLFTIAMAGPTQDVRIPRNRAVVMLV
                     IDVSQSMRATDVEPNRMAAAQVAAKQFAGELTPGINLGLIAYAGTATVLVSPTTNRDA
                     TKLAIDKLQFADRTATGEGIFTALQAIATVGAVIGGGDTPPPARIVLFSDGKETVPSN
                     PDNPKGAYTAARTAKDQGVPISTISFGTPYGYVEINDQRQPVPVDDETMKKVAELSGG
                     ESFSASNLSELKSVYATLQEQIGYETIKGDASTGWLRLGSLVLALSALAALLINRRLP
                     N"
     gene            4628..5398
                     /gene="fabG1"
                     /locus_tag="VF874_03470"
     CDS             4628..5398
                     /gene="fabG1"
                     /locus_tag="VF874_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG1"
                     /protein_id="HEX9175179.1"
                     /translation="MTVTDNPAETAGQVDAARPQFVSRSVLVTGGNRGIGLAVARRLA
                     ADGHKVAVTHRGSGVPDEDLFAVECDVTDSAAVDAAFTEVEEHQGPVEVLVANAGVTA
                     DMLIMRMSEENFEKVINANLTGVFRVAKRASRNMIKKRFGRLIFMGSVVGLTGLPGQA
                     NYAASKAGLVGIARSLTRELGSRSITANVVAPGFIDTDMTRALSDQHQETALQAIPLQ
                     RIGRVEEVAGVVSFLASEDAGYISGAVIPVDGGLGMGH"
     gene            5409..6218
                     /gene="inhA"
                     /locus_tag="VF874_03475"
     CDS             5409..6218
                     /gene="inhA"
                     /locus_tag="VF874_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent enoyl-ACP reductase InhA"
                     /protein_id="HEX9175180.1"
                     /translation="MEKLLAGKRILVTGIITDSSIAFHIARVAQEAGAELVLTGFDRM
                     RLIQRIVDRLPQKAPLLELDVQNEEHLATLADRVSEVIGAGNKLDGVVHSIGFMPPSG
                     MGINPFFDAPFGDVAKGIHISAYSYASLAKAMLPIMNPGGGIVGMDFDPTRAMPAYNW
                     MTVAKSALESVNRFVAREAGKYGVRSNLVAAGPIRTLAMSAIVGGALGDEASGQMQLL
                     EEGWDQRAPIGWNMKDATPVAKTVCALLSDWLPATTGTVIYADGGASTQLL"
     gene            6218..7270
                     /locus_tag="VF874_03480"
     CDS             6218..7270
                     /locus_tag="VF874_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX9175181.1"
                     /translation="MGDEPLARRAFGSVDALLLLSFGGPEGPEQVRPFLENVTRGRGV
                     PPERLDDVAQHYLHFGGVSPINAINRALIEQIQAELADRGSDLPVYFGNRNWEPYVED
                     TVARMRDDGVRRAAVFATSAWGGYSSCRQYLEDIARARRAAGENAPELVKLRHYYDHP
                     LLVEMFADGIATAAATLPDEARAQARLVFTAHSIPVRATLACGPDLYSRQVAHASRLL
                     AEAAGYSSYDQVWQSRSGPPQVPWLEPDVADHLTTMAAQGTAAVIICPVGFVADHIEV
                     VWDLDHELRQQAEAAGIAFARASTPGADRRFAKLAVDLIDELRYGREPERLPSRDAPP
                     LHGFSVNGAPCTPDCG"
     gene            complement(7455..8294)
                     /locus_tag="VF874_03485"
     CDS             complement(7455..8294)
                     /locus_tag="VF874_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175182.1"
                     /translation="MWCASVSLSVWANAWLAGSAAPDDVLDALSLWAPKHLVAAYDSV
                     TAGHTGLPWPDLDEASAVMLLQTLRTTAARPGASEASPSALRASGSSPISVVLPVPGD
                     VRGLTAGTQFEHDALAAGEAVIVAGPRGTAIGLVPEFDYGDDESDADSPPRALVWTVY
                     SLPAAPLPDHPDLGEAEYALRSAVRSAADAFGAIRPGFDIADPRGMVEQLLESASAHR
                     VPEDAPSRALRVLENAAHVDAILAVSSGAQIASDAFTPLSAVVRSARMAAVNAILQSA
                     WRR"
     gene            8354..8785
                     /locus_tag="VF874_03490"
     CDS             8354..8785
                     /locus_tag="VF874_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX9175183.1"
                     /translation="MPVALVWLIVALALAGAEVLTGDLFLLMLGGGALAAAGSTLLFD
                     DLWVHGVVFLVASILLLVGVRPVLRRHLSSGKGLPEPMKELEGRSALVLDRVARHEGQ
                     VKLEGQVWTARPLNESDVYQPGDHVTVVHIDGATAVVSKID"
     gene            8823..10040
                     /locus_tag="VF874_03495"
     CDS             8823..10040
                     /locus_tag="VF874_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9175184.1"
                     /translation="MDGAVVGLVFLAVLVVFAIIVVAKSVALIPQAEAAVIERLGRYS
                     KTVSGQLTLLLPFIDRIRARVDLRERVVSFPPQPVITEDNLTVNIDTVVYFQVTNPQA
                     AVYQISNYIVGVEQLTTTTLRNVVGGMTLEQTLTSRDQINGQLRGVLDEATGRWGLRV
                     ARVELRSIDPPPSIQDSMEKQMRADREKRAMILTAEGSREASIKQAEGQKQAQILAAE
                     GAKQAAILAAEADRQSRMLRAQGERAAQYLQAQGQAKAIEKTFAAIKAGRPTPEMLAY
                     QYLQTLPLMAKGEANKVWIVPSDFGSALQGFTKMLGAPGEDGVFRYQPSPVDDNLPKP
                     EDDSEEVADWFNTQTDPEIAQAVAAAVADARSSDVGAIGTAPVDLLPPMQYPPLSQQQ
                     QSPGPRHGGSLDL"
     gene            10037..10402
                     /locus_tag="VF874_03500"
     CDS             10037..10402
                     /locus_tag="VF874_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9175185.1"
                     /translation="MRSEAVLTSRRTYVALGAFQAGDAVACAIPLPILAKILDDLSIP
                     PGIRWVIPVAKAVAALGLLSVLWFPGLARLTTAMLTLYYVLAVGAHVRARDKVVNAVP
                     AAAFLATFAVMTAKGPEAG"
     gene            complement(10329..>10688)
                     /locus_tag="VF874_03505"
     CDS             complement(10329..>10688)
                     /locus_tag="VF874_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX9175186.1"
                     /translation="DARFRQRGWAIVLSLRLIPAVPFSVINYAAGASAVRVSPYTVAT
                     FVGLIPGTLAFVVLGDAVTGRVNPLLVVVSAVFACVGVTGLVYEIRSHGPAASLPPAP
                     SPSSPRTWPETPLPEPH"
BASE COUNT         1797 a         3504 c         3700 g         1687 t
ORIGIN      
        1 tgaagcccac cgacacgggc atcaccaacg ggcagatctc gcgggtgtac gggcggcagg
       61 cctccgagta cgtgatccag cggggcatgt cgcagatcgg cgtgccgtac tcgtggggcg
      121 gcggaacggc cgcggggaaa agcatcggcc aaggcgacgc cacggtgggc ttcgactgct
      181 caggactggt tctgtactcg ttcgccggag tcgggatcaa gctgccgcac tactcgggcg
      241 ctcaatacga catggggcgc aagatcccag ccgctcagat gcgccggggc gacgtcatct
      301 tctacggacc cggcggcagc cagcacgtga ccatctacct cggcaaggat gcgagcggag
      361 tcggccaaat gctggaggcc cagcaaaccg gggtgccggt gaaagtctcg ccggtgcgca
      421 ccagtggcat gacgccgtac gtcgtgcgct acatcgaata ctgaccagga ggggaagcgc
      481 ttttgcgtcc gactcgtctg ctgcggaccg tctgtgtggt cctggcgccg ctgctgattg
      541 cgttcgcgac accggcggcg gccgagcctg acggctggga tccgacactg ccccagatca
      601 tcagcgccgg cgcgcccggc gacccgctgg cgatcgccaa cgcgtcgctg caggccagcg
      661 cccaggccgc gcagacgacg atggacctcg gccgcaagtt cctcggcagc ctcggcctgt
      721 tccccaactc cacggctgcg ccgggccggg tgtacggcgc gcaggctatt gaatatgtga
      781 tccgccgcgg cgggtcgcag ataggggtcc cgtactcctg gggcgggggc agccttgacg
      841 gtccgaccac cgggatcgac tccggagcag gcaccgtcgg cttcgactgc tcggggctga
      901 tgcgctacgc gttcgccggc gtcggtgtgc tgatcccgcg gttctccggg gaccagtaca
      961 ccgcgggccg gcacattccg ccctcccagg ccaagcgggg cgacctgatc ttctacgggc
     1021 ccggcggtgg ccagcacgtc acgatgtacc tgggcaacgg ccagatgctg gaggcgtcat
     1081 cgctggccgg caaggtcacg gtcagcccag tgcgcacggc cggaatgacg ccgtacctga
     1141 cgcggatgat cgagagctga gccgacacac acagccgccc gccaggcaac ctacgtcgac
     1201 ggaagtccag acgcctggaa tagttgacgg cggacacagg tctatcgaca gcgctggagg
     1261 atcttgatga cgtcaccagg tgggccgccg cagggcgccg gaactttttc ggggccaggt
     1321 ggggctcccg gttacccggg tgcgcagacc gcacccgcgg ctgctccacc gcctgagcgc
     1381 aacggcgggc tcgcctcgga ggtgcacacg ctggagcgag ccgtctacga ggtcaagcgg
     1441 atcatcgtcg gccaggacca gctcgtcgaa cggatgctgg tcgggctgct ggccaagggc
     1501 catgtgctgc tcgaaggtgt gcccggcgtc gccaagacac tggcggtcga gacgttcgcc
     1561 cgcgtggtcg gcggcacctt cgcccgtatc cagttcacgc cggacctggt gcccaccgac
     1621 atcgtcggca cccgcatcta ccgccagggc aaggaggagt tcgacatcga gctcggcccc
     1681 gtcgtggtga acttcctgct ggccgacgag atcaaccgcg cgccggccaa ggtgcagtcc
     1741 gcgctgctcg aggtgatgca ggaacgcagg atctcgatcg gcggcaagga cttttcgatg
     1801 ccgaacccgt tcctggtgat ggccacccag aacccgatcg agcaggaggg tgtgtacgcg
     1861 ctgccggagg cgcagcgcga ccgcttcctc ttcaagctga acatcgacta cccgtcaccc
     1921 gaggaagagc gcgagatcat ctaccggatg ggcgtgaagc cgccggagcc caagcagatc
     1981 ctcgctcccg gtgacctgct gcggctgcag gacgtcgcga gcaacatttt cgtccaccac
     2041 gctcttgtcg actacgtcgt gcggatcgtc actgccaccc gccggccgga ccagttcggc
     2101 atgcccgacg ccagggcctg gatcgcctac ggcgcgtcgc cgcgcgcctc gctcggcatc
     2161 atcgcggccg ctcgggcgct ggcgctggta cgcggccgcg actatgtgat cccgcaggac
     2221 gtcatcgagg tcatccccga cgtgctgcgg caccggctgg tgctgacgta cgacgcgctg
     2281 gccgacgaga tctcgccgga gaccgtgatc acccggatca tgcagaccgt cgccctaccg
     2341 caggtcaatg ccgttccgca gcaagggcat tcggtgcatc ccgtggtgcc cgccgcggcg
     2401 gctgcggcta gtggtcggtg agtccgtccg ccggtcggac cgtcgacctt ccgtcgttgc
     2461 agcgcggtca gatccgcgac cccgagctgt cggcggcgct gcgcaaactg gaactcacag
     2521 tccggcgcaa gcttgacggc gtgctccacg gcgaccacct cggcctgatt ccggggccgg
     2581 gcagcgagcc gggtgagtcg cggctctacc agcccggcga cgacgtccgc cggatggact
     2641 ggtcggtgac cgcgcgcacc aaccacccgc acgtcaggca gatgatcgcc gaccgcgagc
     2701 tggagacgtg gctggtggtc gacatgtcgg ccagcctgga cttcggcacc acgggctgcg
     2761 agaagcgcga tctggccgtg gccgctgccg cggcgatcgc gttcctcaac agtggtggcg
     2821 gcaaccggct cggtgcgctg attgccaacg ggcaggacat cattcgggtg ccggcgctgt
     2881 cgggccggat gcatgaacag acgctgctgc gagcgatcgc gacctcgccg aaggctcccg
     2941 tgggtgtacg cggggatctc gccgccgcga tcgacgacct gcgccggccc gagcgccgac
     3001 gggggatggc ggtgatcatc agcgacttcc tcggaccgat caactggatg cgcccgctgc
     3061 gcgcgatcgc cggccgtcac gaagtgctcg gcatcgaagt cctcgacccg cgcgacgtcg
     3121 agctgcctga cgtcggcgac gtgatcctgc aggacgccga gaccggcgtc acccgagagt
     3181 tcaccatcga cgccaagctg cgcgacgact tcgccaaggc cgcggcggtt caccgggcag
     3241 aggtggcccg caccctgcgg cgctgcgatg caccactgct gtcgctgcgc accgatcgtg
     3301 actggattgc ggacgtggtg cggttcgtca gcagccgacg tcggggagcg atggcggggc
     3361 gatgagcgct tgcgcgaaga gcagaaggca gcgatgagcg cttgcgcgaa gagcagaagg
     3421 cagcggtagc ggccccaaat caacacgaat gaaatgacaa agctgaaatg acatcgccta
     3481 atcacgcgtg tgtaatgaca cggctcggat gcgcatgaac gtgccattgt tagggccgat
     3541 gacgctgacg ggcttcgccc acacgtggtg gttcctcttt ctgctcgtcg tcgtcggcct
     3601 ggttgtggca tacgtcctcg cgcagctggc ccgccaacgc cgcgtgttgc ggttcgcgaa
     3661 catggaactg ctggaaagcg ttgcgccaca acggccgaac agatggcggc acctgcccgc
     3721 gattcttctg gtggcgtcgc tggttctgtt cacgatcgcg atggccggac ccacgcagga
     3781 cgtgcggatc ccgcgcaacc gtgccgtcgt gatgctggtg atcgacgtct cgcagtcgat
     3841 gcgtgccacc gacgtggagc cgaaccggat ggccgcggcg caggtggcgg ccaagcagtt
     3901 cgccggcgag ctgacccccg gcatcaacct cggtctgatc gcctacgccg gcaccgcgac
     3961 ggtgctggtg tcgccgacga ccaaccgtga cgccaccaaa ctcgcgatcg acaaactgca
     4021 gttcgccgac cgaaccgcga ccggcgaagg catcttcacc gcgttgcagg cgatagcgac
     4081 cgtcggcgcg gtgatcggcg gcggcgacac cccgccgccg gcgcgaatcg tgctgttctc
     4141 cgacggtaag gaaaccgtgc cgtccaaccc ggacaacccg aagggtgcct acaccgcggc
     4201 gcgcaccgcc aaggaccagg gcgtgccgat ttcgacgatc tcgttcggca cgccgtacgg
     4261 gtacgtcgag atcaacgacc agcgccagcc ggtgcccgtc gacgacgaga cgatgaagaa
     4321 agtcgcggag ctctccggcg gggagtcctt cagcgcgtcc aacctctccg agctgaagag
     4381 cgtctacgcg acgctgcagg agcagatcgg ttacgagacc atcaagggcg acgccagcac
     4441 gggctggctg cggctcggtt cgctggtact agcgctttct gcattggcgg ctctgctgat
     4501 caatcgccgg ctgccgaact aggtctgcgt tgttgcgccg agatctacgt cagggtcgtg
     4561 atttctaccc aacaacagcc ctggtgtcga tttcggcgtg cgcacgtcac ccgttaagtt
     4621 gacgggcatg actgtgaccg acaatccagc cgaaaccgcg ggtcaggtcg acgccgcccg
     4681 tccgcagttc gtctcgcgtt ccgtgctcgt cacgggcgga aaccggggca tcggcctcgc
     4741 tgtggcccgc cgtcttgcag ccgatgggca caaggtcgcc gtcacgcacc gcggctcagg
     4801 cgtgcctgac gaggacctgt tcgcagtcga gtgtgacgtg accgacagcg ccgctgtcga
     4861 cgccgcgttc accgaggtgg aagagcacca gggtcccgtc gaggtgctcg tcgcgaacgc
     4921 cggggtgacc gcggacatgc tgatcatgcg gatgagcgaa gagaacttcg aaaaggtgat
     4981 caacgcgaac ctcactggtg tgttccgggt ggccaagcgg gcgtcgcgca acatgatcaa
     5041 gaagcgcttc gggcgactga tcttcatggg ctcggtcgtc gggctgaccg gtctgcccgg
     5101 ccaggcgaac tacgcggcat ccaaagccgg actggtcggc atcgcgcgct cgctgacccg
     5161 ggagctgggc tcgcggtcga tcaccgcgaa cgtcgtcgca cctggcttca tcgacaccga
     5221 catgacgcgc gcgctgtccg accagcacca ggagaccgca ctgcaggcga ttccgctgca
     5281 acgcatcggc cgcgtcgaag aagtcgccgg agtggtcagc ttcctggcat ccgaggatgc
     5341 cggctacatc tccggtgcgg tgatcccggt cgacggcggc ctgggcatgg gccactgaga
     5401 ggacggacat ggaaaagctg ctcgcgggca aacgaattct cgtcacgggg atcatcaccg
     5461 actcgtcgat cgcctttcac atcgcacgtg tggcgcagga ggcgggcgcg gagctggtgc
     5521 tgaccggttt cgaccggatg cggttgattc agcgcatcgt cgaccggttg ccgcagaagg
     5581 caccactgtt ggaactcgac gtgcaaaacg aggagcatct ggccacgctt gccgaccgcg
     5641 tgtcagaagt gatcggtgcg ggtaacaaac tcgacggtgt cgtgcactcg atcgggttca
     5701 tgccgcccag cgggatgggc ataaacccgt tcttcgacgc gcctttcggg gatgtcgcca
     5761 agggcatcca catctccgcg tactcgtatg cgtcgctggc caaggcgatg ctgccgatca
     5821 tgaatcccgg cggcggcatc gtcggcatgg acttcgaccc gacccgtgcg atgccggcct
     5881 acaactggat gacggtcgcc aagagtgcgc tcgagtcggt caaccgcttc gtcgcccgcg
     5941 aggccggcaa gtacggtgtg cggtccaatc tcgttgcggc aggaccgatt cggacgctag
     6001 ccatgagcgc gatcgtcggc ggcgcacttg gcgacgaggc cagtggtcag atgcagctgc
     6061 ttgaagaggg ctgggatcaa cgggccccga tcggttggaa catgaaggac gcgacgccgg
     6121 tggcgaagac ggtgtgcgcg ctgctctccg actggctgcc cgcgaccacc gggaccgtca
     6181 tctacgccga cggcggcgcg agtacccaac tcctgtaatg ggcgatgagc cgcttgcgcg
     6241 aagagcattt ggctccgtcg acgccctcct gctgctgtcg ttcggcgggc cggagggccc
     6301 cgagcaggtg cggccgttcc tggagaacgt cacccgcggt cgcggcgtgc cgccggagcg
     6361 gctcgacgac gtcgcgcagc actacctgca tttcggtggc gtgtcaccga tcaacgcgat
     6421 aaaccgcgcg ctgatcgaac agatccaagc cgagctcgcc gaccgcggca gcgacctgcc
     6481 ggtgtacttc ggcaaccgca actgggaacc gtacgtcgag gacaccgtcg ctcggatgcg
     6541 tgacgacggc gtgcgccgcg cggcggtgtt cgcgacgtcg gcgtggggcg ggtactccag
     6601 ctgccggcag tacctcgagg acatcgcgcg ggcgcggcgt gcggcagggg agaacgcgcc
     6661 cgagttggtc aagctgcgtc actactacga ccaccccctg ctggtggaga tgttcgccga
     6721 cgggatcgcc acggccgccg cgacattgcc cgacgaggcg cgtgcgcagg cgcggctggt
     6781 gttcaccgcg cattccatcc cggtgcgggc caccttggcc tgcgggcccg acctgtacag
     6841 ccggcaggtg gcgcacgcgt cgcggttgtt ggcggaagcc gcgggctatt cgtcgtacga
     6901 ccaggtgtgg cagtcgcgtt cgggcccgcc acaggtgccc tggctggaac ccgacgtcgc
     6961 cgaccatctg acgacgatgg ccgcccaggg gactgcggcg gtgatcatct gtccggtcgg
     7021 gtttgtcgcc gaccacatcg aagtggtgtg ggacctcgac catgagctgc ggcagcaggc
     7081 tgaggctgcg ggcatcgcgt tcgcccgcgc gtcgacaccc ggtgccgatc gacgcttcgc
     7141 gaagctggcc gtcgatctga tcgacgagct gcgttacggg cgcgagccgg agcgtctgcc
     7201 ttcgagggat gcacctccgc tgcacggctt ttcagtcaac ggcgcgccct gcacgccgga
     7261 ctgcggatag ctgcggggcg ggaagttgag gtaaggataa ccggccggac ctcaaacatg
     7321 ttgagagcca gccggattca tgatgtagct agctgagcgc agggtgcgca cccccgaaag
     7381 gccgcgaatg tgcgctgttg cacgccattc ctcggcgtgt cggggtacag acacgcacgc
     7441 tcgcggcagg ggacttagcg gcgccaggcc gactgcagga tcgcgttcac ggcggccatc
     7501 cgcgcggagc ggaccaccgc ggataacggc gtgaatgcat cgctggcgat ctgcgcgccg
     7561 gagctgaccg cgaggatcgc gtcgacgtgg gcggcgttct ccagcacccg cagcgcccgc
     7621 gacggcgcgt cctcgggaac acggtgcgca ctcgcggact ccagcagctg ctcgaccatg
     7681 ccgcgcgggt cggcgatatc gaatcccggt cggatcgcgc cgaacgcgtc ggcggctgaa
     7741 cgcaccgcgg agcgcaacgc gtactcggcc tcacccaggt cggggtgatc gggaagtggt
     7801 gcagcgggca gcgaatagac cgtccacacc agtgcgcgcg gcggtgagtc tgcgtcggat
     7861 tcgtcgtcgc cgtagtcgaa ctcgggtacc aaaccaatcg ccgtcccgcg tggccccgcc
     7921 acgatcaccg cctcgccggc ggcgagtgcg tcgtgttcga actgggtgcc ggcagtcaac
     7981 ccccgcacgt cgccggggac gggcagcacg acgctgatcg gcgatgagcc gcttgcgcga
     8041 agagcagaag gggaggcttc gctggcgccg ggtcgcgcgg ccgtggtgcg cagggtctgc
     8101 agcagcatga cggcgctggc ctcgtcgagg tctggccacg gcagcccagt gtgaccggct
     8161 gtcacggaat cgtatgcggc aactaaatgt tttggcgccc agagggataa cgcatcgagg
     8221 acgtcgtcgg gcgcagcaga gccggcgagc caggcgttgg cccacaccga cagcgacacg
     8281 ctcgcgcacc acatgaaggc cgagtgtaat agttcgcccc ggagcccggc tggttacacg
     8341 ataggctggc gtcatgcctg tggcgctggt atggctgatc gtcgcgttgg cgttggcggg
     8401 cgcggaggtg ttgaccggcg acctgttcct gttgatgctg ggcggcggcg cgctggccgc
     8461 ggccgggtcg acgttgttgt tcgacgacct gtgggtgcac ggtgtcgtct tcttggtggc
     8521 gtcgatcctg ctgctcgtcg gggttcgtcc ggtgctgcgc cgacacttga gttcgggcaa
     8581 gggcctgcct gaacccatga aagagctgga gggcaggagc gcgcttgtgc tcgaccgggt
     8641 ggcgcgtcac gagggccagg tgaagctcga aggccaggtg tggacggccc ggccgctgaa
     8701 cgagagcgat gtctaccaac ccggcgacca tgtcaccgtc gtgcacatcg acggcgcgac
     8761 cgcggtcgtc tccaagatcg actagagaaa cctggacgaa gaaggaagga aggaatccca
     8821 ctatggatgg tgctgtcgtc ggcctggttt ttctggccgt tctcgtggtg ttcgcgatca
     8881 tcgtcgtcgc caaatcggtg gcgctgatcc cgcaagccga ggccgcggtg atcgagcggc
     8941 tgggccgcta cagcaagacc gtgtccggtc agctgacact gctgctgccg ttcatcgacc
     9001 gcattcgggc ccgcgtggac ctgcgcgagc gagtggtgtc gttcccgccc cagccggtga
     9061 tcaccgagga caacctgacg gtgaacatcg acacggtggt gtacttccag gtcaccaacc
     9121 cgcaagccgc ggtgtaccag atcagcaact acatcgtcgg cgtcgagcag ctgaccacca
     9181 ccacgctgcg caacgtggtc ggcggcatga cactcgagca gacgctgacc tcgcgagacc
     9241 agatcaacgg ccagctgcgc ggcgtgctcg acgaggccac cggccggtgg gggcttcgcg
     9301 tggcccgagt cgagttgcgc agcatcgatc cgccgccgtc gattcaggac tcgatggaaa
     9361 agcagatgag ggcggaccgc gagaagcgcg cgatgatcct gaccgcggaa ggcagccgcg
     9421 aggcgtcgat caagcaggcc gagggccaga agcaggcgca gatcctggcg gccgaggggg
     9481 ccaagcaggc tgccatcctt gctgcagagg ccgaccgcca gtcccggatg ctgcgcgccc
     9541 agggcgagcg ggccgcgcaa tacctgcagg cgcagggcca ggcgaaggcc atcgagaaga
     9601 cgttcgccgc gatcaaggcc ggccggccga cccccgagat gcttgcctat caatatctgc
     9661 agacgctgcc gctgatggcc aagggtgagg cgaacaaggt ctggatcgtg cccagcgatt
     9721 tcggttccgc gctgcagggc ttcacgaaga tgttgggagc gcccggtgag gacggcgtgt
     9781 tccggtatca gccgtcaccg gtcgacgaca acctgccgaa gccggaggac gactccgagg
     9841 aggtcgcgga ctggttcaac acccagaccg accccgagat tgctcaggcc gtcgcggccg
     9901 ccgtcgcgga tgcacggtcg agtgacgtgg gcgctatcgg cacggctccc gtggacctgc
     9961 tcccaccgat gcagtaccca ccgctgtcgc agcagcagca gtcgccggga ccgcgccacg
    10021 gcggctcgct ggacctgtga gaagcgaagc ggtcctcacc tctcggcgga cctacgtcgc
    10081 gcttggcgcg ttccaggcgg gcgatgccgt ggcgtgcgcg atcccgctgc cgatcctcgc
    10141 gaagattctc gacgaccttt cgatcccacc cggcatccgc tgggtcatcc cggtcgcgaa
    10201 ggccgtcgcg gcgctcggtt tgttgtcagt gttgtggttt cccgggctgg ccaggctcac
    10261 cacggcgatg ttgacgctgt attacgtcct cgccgtgggt gcgcacgtcc gtgcgcgtga
    10321 caaggtcgtc aatgcggttc cggcagcggc gtttctggcc acgttcgcgg tgatgacggc
    10381 gaaggggccg gaggcaggct agcggctgga ccgtgtgacc gaatctcgta caccaggccg
    10441 gtgacgccca cgcaggcgaa gaccgccgac accacgacaa gcagcgggtt gacccgcccg
    10501 gtcaccgcgt cgcccagcac gacgaacgcc agcgtcccgg ggatcagccc gacgaacgtc
    10561 gccaccgtgt acggcgacac tcgcacggcg gatgccccgg cggcgtaatt gatcaccgag
    10621 aacggcaccg ccggaatcag tcgcagcgac aagacaatcg cccagccgcg ctgccggaac
    10681 cgcgcgtc
//
LOCUS       DASWBF010000101         8254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_206360,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000101 DASWBF010000000
VERSION     DASWBF010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206360"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF874_03510"
     CDS             <1..213
                     /locus_tag="VF874_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175187.1"
                     /translation="TIAGFAFGPFTATTTPEIATAVAHLRDVLGDQTYESLARKGETM
                     TMTAMATYAYDQIDQARTGLERPRFK"
     gene            complement(276..422)
                     /locus_tag="VF874_03515"
     CDS             complement(276..422)
                     /locus_tag="VF874_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175188.1"
                     /translation="MNWLLRLRALLAHAHGDETGYRDYRDRYRAMATSLGFEGHMKWA
                     EAMP"
     gene            complement(419..865)
                     /locus_tag="VF874_03520"
     CDS             complement(419..865)
                     /locus_tag="VF874_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175189.1"
                     /translation="MHTTDQIPTAEELNAMTPHEHRLYELKVRRMARRQYLTLIKSRR
                     RDTACAYALIDPYLNMLVAGDHNTGYGLGLDEIHAALLDAEALGVKVAGHPHFPFGYV
                     PLSGQRAVEDSCPSAPAGLTRHMADRVGLGTGPGARTARIAGVAQS"
     gene            1016..1171
                     /locus_tag="VF874_03525"
     CDS             1016..1171
                     /locus_tag="VF874_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175190.1"
                     /translation="MSLPPAYPCSSSITESCPNIRIPMPGEDCYAALVWLAEHATDLE
                     SIRPVWG"
     gene            1168..1641
                     /locus_tag="VF874_03530"
     CDS             1168..1641
                     /locus_tag="VF874_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX9175191.1"
                     /translation="MIGDSAGSGLSAALALLARDRGRPALAQQILIYPMLDDRVPEPD
                     PQLVPFLVFSYDDNAIGWEALLGDHAGKEGVSPYAAPARADDLGDLPDAYIEAAQLDV
                     LRNEAIAYAQRLTDPGVPAELHVHPGCPHGFDLIAPNAGVSQHAVADRIRRIRSL"
     gene            complement(1761..2060)
                     /locus_tag="VF874_03535"
                     /pseudo
     CDS             complement(1761..2060)
                     /locus_tag="VF874_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heparin-binding hemagglutinin"
     gene            2242..2619
                     /locus_tag="VF874_03540"
                     /pseudo
     CDS             2242..2619
                     /locus_tag="VF874_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856702.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2629..4089
                     /locus_tag="VF874_03545"
     CDS             2629..4089
                     /locus_tag="VF874_03545"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HEX9175192.1"
                     /translation="MTADSTLDTAAHSAEYVDAASAAFRAALQVMESVEPRIAAATRK
                     ELADQRDSLKLIASENYASPAVLLTMGNWLSDKYAEGTIGHRFYAGCQNVDTVESVAA
                     EHARELFGSPYAYVQPHSGIDANLVAYWAILATRVEAPSLAELGAKHVNDLSEADWET
                     LRAKLGNQRLLGMSLDAGGHLTHGFRPNISGKMFYQRSYGTDPATGLLDYDAVAAAAR
                     EFKPLILVVGYSAYPRRVNFAKMREIADEVGATLMVDMAHFAGLVAGKVFTGDEDPVP
                     YAHVTTTTTHKSLRGPRGGLVLATEEYSGAVDKGCPMVLGGPLSHVMAAKAIALAEAR
                     QPAFGAYAQRVADNAKALADGFLRRGARLVTGGTDNHIVLLDVTSFGLTGRQAESALL
                     DAGIVTNRNAIPADPNGAWYTSGIRFGTPALTTRGFTEAEFDRTAELVVDVLTDTSPQ
                     GTSKAKYSLVDATGDWVRAAVGELLDANPLYPGLNL"
     gene            4394..5218
                     /locus_tag="VF874_03550"
     CDS             4394..5218
                     /locus_tag="VF874_03550"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX9175193.1"
                     /translation="MAQKPVLTPLMAELEPVVGENYHRHIDTAEPWYAHDFVPFDRGE
                     NFKFLGGNDWDPSQATLPRHITDALEILLVTKDNLSGYHREFVEHFILEDWWGMWIGR
                     WTAEETLHQIALREYLVVTREIDPVANENVRVEHVMKGYRADSFGKIETLVFMALRER
                     EHAVYCRRLAAQIEDATLRSLVEAIAADEERHLQFFENLVRACLNLEPEETIAAIAAR
                     AAELQVVGADIDAYKDKVATVAETGVYDENLMRAGIADSIAAWGLTDRPELTDFVR"
     gene            5394..6713
                     /locus_tag="VF874_03555"
     CDS             5394..6713
                     /locus_tag="VF874_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9175194.1"
                     /translation="MTNSPSPLRTYVLDTSVLLSDPWAATRFAEHEVVVPLVVISELE
                     AKRHHHELGWFARQALRLFDDLRLEHGRLDQPIPVGTQGGTLHVELNHSDPAVLPAGF
                     RTDTNDARILTCAANLAAEGKQVTLVSKDIPLRVKAGAVGLTADEYHAQDVIVSGWTG
                     MAELDASAADIDALFADGEIDLQAARELPCHTGTRLLGGGSHALGRVNAGKRVQLVRG
                     DREVFGLRGRSAEQRVALDLLLDESVGIVSLGGKAGTGKSAMALCAGLEAVLERRTHR
                     KVVVFRPLYAVGGQDLGYLPGSESEKMGPWAQAVFDTLEGLASPAVLEEVLSRGMLEV
                     LPLTHIRGRSLHDSFVIVDEAQSLERNVLLTVLSRLGAGSRVVLTHDVAQRDNLRVGR
                     HDGVGAVIEKLKGHPLFAHITLVRSERSPIAALVTEMLEEISPGALP"
     gene            6775..7617
                     /locus_tag="VF874_03560"
     CDS             6775..7617
                     /locus_tag="VF874_03560"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9175195.1"
                     /translation="MTLCAAAGAVAVLVFGSLTGHVKLTSAADVDCSKVKCVAFTFDD
                     GPGPYTDRLLQILNDNDAKATFFLIGNKVAANPAGAKRIVDAGMEVGSHTWEHPNMTT
                     IPSDAIAGQLSKASDAIASATGQTPKLWRPAGGLTNDEVNNVAAQQGLAAILWDVIPF
                     DWINDSDTAATRYMLMSQIKPGSVVLFHDTYSSTVDLVYQFLPVLKANGYHLVTVSQL
                     LGPRAPGSVYGSRDNGPPVNDLHDVPPADIPTLPNTPSPKPMPNFPITDLPDQNSGGP
                     NNGA"
     gene            7642..7959
                     /locus_tag="VF874_03565"
                     /pseudo
     CDS             7642..7959
                     /locus_tag="VF874_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
     gene            complement(7968..>8254)
                     /gene="aspA"
                     /locus_tag="VF874_03570"
     CDS             complement(7968..>8254)
                     /gene="aspA"
                     /locus_tag="VF874_03570"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706050.1"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX9175196.1"
                     /translation="NVSRLFAERCIDGLIAHEDRLRTLAESSPSIVTPLNSAIGYEEA
                     AAVAKEALKEHKTIRQTVIDRGLIGDKLSEEELDKRLDVLAMAKVKPQEA"
BASE COUNT         1417 a         2742 c         2682 g         1413 t
ORIGIN      
        1 accatcgccg gtttcgcgtt cggtcccttc accgcgacga cgacccctga aatcgccacc
       61 gctgtcgccc acctccgcga tgtcctcggc gaccagacct acgaatcgct cgcccgcaag
      121 ggcgagacga tgaccatgac agccatggcg acgtacgcat acgaccaaat cgaccaggcc
      181 cgcacaggac tcgaacgccc gcgattcaag tgacgggacc ggggagatga cgacgggcga
      241 ctagaccaaa ggtgagggta cggaaacgac cgccgtcacg gcatcgcctc ggcccacttc
      301 atgtgcccct cgaagcccaa tgatgtcgcc attgcgcggt agcggtcccg atagtcgcga
      361 tagccggtct cgtcgccgtg ggcgtgggcc aacagtgcgc gcagccgcag cagccagttc
      421 acgactgcgc cactccagca attcgagcag tgcgtgcgcc cggtccggtg ccaaggccca
      481 ctcggtcagc catgtgccgc gttaggcccg ccggggcact cgggcacgaa tcttcgacag
      541 cacgctgtcc actcaaggga acatacccaa atgggaaatg cgggtgacca gcgactttca
      601 ctcctaacgc ttctgcatct agcagtgcgg cgtggatctc atcgaggccc aggccgtagc
      661 cggtgttgtg gtcaccggcc acaagcatgt tgaggtaggg atcaatcagc gcgtaggcgc
      721 acgccgtgtc gcgccgcctc gacttgatga gcgttaggta ttgccttcgc gccatgcggc
      781 gcactttcaa ctcgtacagc cgatgctcgt gcggcgtcat cgcgttgagt tcctcggcgg
      841 tggggatctg atcggtggtg tgcatagcca gcttcctgtt cgggcatgtc cgcaaaccat
      901 gcgcgaacgg aggggtgggc atcacgccat cgccgcctac tggctttcgg tgctgacgct
      961 acgcccggcg agccaaatcg aacagcggtt aagcgtccgg gttgaccttc cggctatgtc
     1021 gctgcctccg gcgtacccgt gctcgtcgtc gattaccgag tcgtgcccga acatccgcat
     1081 cccgatgcct ggtgaggact gctacgccgc cctggtgtgg ctcgccgagc acgcgacgga
     1141 tctggaatcg atccggcccg tctgggggtg atcggtgaca gcgcgggcag cggactatct
     1201 gcggccttgg ccttgttggc ccgcgatcgc ggccgtccag ccctagccca acagatattg
     1261 atctacccga tgctcgatga tcgggtgcca gaaccagatc cccagctggt cccgtttctg
     1321 gtattcagct acgacgacaa tgccatcggc tgggaagccc tgctcggaga ccacgcaggc
     1381 aaagagggtg tcagtcccta tgccgccccg gcccgagccg acgaccttgg cgacctgcct
     1441 gacgcctaca tcgaagccgc ccagcttgat gtcctccgca atgaggccat cgcgtacgct
     1501 caacgactga cagaccctgg cgtgccagca gagctgcacg tgcacccggg atgcccgcac
     1561 ggcttcgact taatcgcacc caacgcgggc gtatcccaac acgcagtcgc cgaccgcatc
     1621 cgccgtatcc gttcgctcta aacgcgctgt ttcaacagca cattcaacat tgaacttcac
     1681 ggcgttgccg ccgatgactt cggttacttc ttggtgctct tcttcgccgg aaccctcttg
     1741 gccggtgctg ccttcctggt ccgcgtccgc agtcgctcca tcgcggcctc cccgcgctcc
     1801 accaggctct cgtaggcctt acgcagttcc tcgccgctca gctgctcccg tagctcctcg
     1861 cggcgcttgg tcagccgttc acgggtctcc tcggcccgcg tgctggcatc gctacgggcc
     1921 tcctcggagc gctcgcgcac agcgtcgatg atctcgttga gctcctcgag ggcaagatcg
     1981 accgcgccga cggcggcgag caacgggtcc ttgagatcgt cgatggtcgg ggccttccgg
     2041 ggggctttct tctcggccat gtcaatccct tcgatccagt tcagtgtgtg gtgattcaag
     2101 caaaatcttg ggttggtgaa taggtaaccg aaaacgcccg gcagactcag gacgtgatac
     2161 cggggcttcc acacggcacg cccacagtag tccaacgctg ctttcaaccg tgtggaattc
     2221 cgatttcaaa caacgtctgg cgcgtccgcg gcgctgatcg atgtgcgcca ggacgccgcg
     2281 ttgatgctgc acgacgagca ccgcgacgtc gatgacgtgg ccgcgttctt gaagcggtgg
     2341 ttgctggtcg acgacgtgcg ggcgcggcag atgctgcgtt tcctgtcgtc gccgctgtgg
     2401 cgcgcgtaca tcagcaccta cgtcgagggg tatcggctgc tgcgcgggtg gctcgatgcc
     2461 gctccggccg gcgtcagcgt cgtctcgcgg ttcacccggc tgcttggcga gccgctgatc
     2521 ccgtcggcgc tgcggccgtc gtcgacgctg cgttttcata cgggacacgc cgacggttcg
     2581 tacgaaacca cagagtcggc ggacattcgg gagtcgtagg ctaaacccat gaccgcagac
     2641 tcgacattgg acaccgcagc gcacagcgcc gagtacgtcg acgcagcgag cgcggccttc
     2701 cgagcagcac tgcaagtcat ggagtccgtc gaaccacgga tcgccgccgc gacgcgcaaa
     2761 gagcttgcag accaacgtga ttcgctgaaa ctgatcgcca gcgagaacta cgcgtcgcct
     2821 gctgtgctgc tgaccatggg caactggctc tccgacaagt acgccgaggg cacgatcggg
     2881 caccggttct atgcgggctg ccagaacgtc gacaccgtgg aatccgtggc cgccgagcac
     2941 gcccgcgaac tgttcggctc gccgtacgcg tacgtccaac cgcactcggg catcgacgcc
     3001 aacctggtcg cgtactgggc cattctggcg acccgcgtcg aggcgccaag ccttgccgaa
     3061 ctcggcgcca agcacgtcaa cgacctgtcg gaggccgact gggagacgtt gcgggccaag
     3121 ctcggcaacc agcggctgct gggcatgtcg ctggacgccg gcgggcacct cacccacggc
     3181 ttccggccga acatctccgg caaaatgttc taccagcgca gctacggcac cgaccccgcc
     3241 accggcctcc tcgactacga cgccgtcgcc gccgcagcgc gcgagttcaa gccgctgatc
     3301 ctggtcgtcg ggtactccgc gtacccacgg cgggtcaact tcgcgaagat gcgcgagatc
     3361 gccgacgaag tcggtgcgac gttgatggtc gacatggcgc acttcgccgg tctggtggca
     3421 ggcaaggtgt tcaccggcga cgaggatccg gtgccgtacg cccacgtcac cacgacgacc
     3481 acgcacaagt cgctgcgggg accgcgcggc ggacttgtgc tggccaccga ggagtactcc
     3541 ggcgccgtcg acaagggctg cccgatggtg ctgggcggac cgctgtcgca cgtgatggcc
     3601 gccaaggcga tcgcgctggc cgaggcccgt caaccggcgt tcggcgctta cgcgcagcgg
     3661 gtcgccgaca acgccaaggc gctggccgac ggcttcctgc ggcgcggtgc gcgtctggtc
     3721 accggcggca ccgacaacca catcgtgctg ctcgacgtga cctcgttcgg cctgaccggg
     3781 cgccaagctg agtccgcgct gctcgacgcg ggcatcgtca ccaaccgcaa cgcgattcca
     3841 gcggacccga acggcgcctg gtacaccagc ggcatccggt tcggcacgcc cgcgttgacg
     3901 acacgtggct tcaccgaggc cgaattcgac cgcaccgccg agctggtggt cgacgtgctg
     3961 accgacacgt ctccgcaggg cacgtcgaag gcaaagtact cgctggtcga cgccaccggg
     4021 gactgggtcc gagcggccgt aggtgaactg ctggacgcca atccgctgta tccggggctc
     4081 aacctctagc gagcccgctg aacgagctgg cggcaatcag tcggtcccgt gttcggcagg
     4141 ttcatggcgg aggcgttcgg tgggatagtt cggtttgccg gcctctgcgc gacaacagca
     4201 tttacccagg tcacggccat aacgagagca gatcgatcag cgacaacgca gggcttgcac
     4261 cgcaggtcaa cgagttgtcg cggagaggcg ggcaccaagg tagtcgccca gaaagagctc
     4321 cgcagcgacg cgagagccaa tgtccggcgc gttggatttt acgaaaccct tagaattggg
     4381 ttactgtaac tgcatggcac agaaacctgt tctgacaccg ctcatggcgg agctggaacc
     4441 ggtggtcggg gagaactacc accggcacat cgacaccgcc gaaccctggt acgcgcacga
     4501 cttcgtgccg ttcgaccgcg gcgagaattt caagttcctc ggcggcaacg actgggatcc
     4561 gtcccaggca acactgccca ggcacatcac cgatgcgctg gagattctgc tggtcaccaa
     4621 ggacaacctc tccggttacc accgcgagtt cgtcgagcac ttcatcctcg aggactggtg
     4681 ggggatgtgg atcggccgct ggaccgccga ggagaccctg caccagattg cgttgcggga
     4741 gtacctcgtc gtcacccgtg aaatcgaccc ggtggccaac gagaacgtcc gcgtcgagca
     4801 cgtgatgaag ggctatcgcg ccgacagctt cggcaagatc gagacgctgg tgttcatggc
     4861 gttgcgcgaa cgcgagcatg ccgtgtactg ccggcggctg gccgcgcaga tcgaggacgc
     4921 gacgctgcgc agcctggtcg aggcgatcgc cgccgatgag gagcggcacc tgcagttctt
     4981 cgagaacctg gtacgggcgt gcctgaacct ggagccggag gagacgatcg cggcgatcgc
     5041 cgcacgggcg gccgagttgc aggtcgtcgg cgctgacatc gacgcctaca aggacaaggt
     5101 ggccaccgtc gccgagaccg gtgtctacga cgagaatttg atgcgtgccg ggatcgccga
     5161 ttcgatcgcg gcgtggggtt tgaccgaccg gcccgaactc actgatttcg ttcgctgatc
     5221 cgtctgctct tcgcgcaagc ggctcatcgc tggcgcacgg cgcgcctgcg gggcggtgac
     5281 ctggcgacgg gggtagcgtc gcatccgatg gctagcgaca tgctctgcta tccgggcggc
     5341 ccccggcctc accacgccga cccgcggggc cgcgcgggct cgaggagctg tccgtgacca
     5401 attcgccgtc acctcttcgg acctacgtgc tggacacgtc ggtgctgctg tccgacccat
     5461 gggcagcgac tcggttcgcg gagcacgaag tggtggtccc gctggtggtg atcagcgaac
     5521 tggaggccaa gcgccatcac catgagctgg gctggttcgc ccgccaggcg ctgcgtctct
     5581 tcgacgatct gcgtctcgag cacggccgct tggatcagcc gattcccgtt gggacacaag
     5641 gcggaacgct tcacgtcgag ctgaaccaca gcgacccggc cgtgttgccg gcgggatttc
     5701 gcaccgacac caacgacgct cggatcctga cgtgtgcggc caacctcgcg gccgagggca
     5761 agcaagtcac gttggtgagc aaggacattc cgctgcgtgt caaagccggg gcggtcgggc
     5821 tgaccgccga cgaataccac gcacaggacg tgatcgtctc aggctggacc gggatggccg
     5881 agctggacgc atccgccgcc gacatcgacg cactgttcgc cgacggcgag atcgaccttc
     5941 aggcagcacg agagctgccg tgccacaccg gaactcggct tctcggtggc ggctctcacg
     6001 cgctcgggcg ggtcaacgcc ggtaagcggg tgcagctggt ccgcggtgac cgcgaggtgt
     6061 tcgggctgcg cggccggtcg gccgagcagc gggtcgcgct ggacttgctg ctagacgaat
     6121 cggtcgggat cgtgtcgctg ggcggcaagg ccgggaccgg caagtcggcg atggcgctgt
     6181 gcgcgggatt ggaggccgtg ctggagcggc ggacacaccg caaggttgtg gtgttccggc
     6241 cgctgtacgc cgtcggcggt caggatctcg gctacctgcc cggcagcgag agcgaaaaga
     6301 tgggcccgtg ggcgcaagcc gtcttcgaca cgctggaggg gctggccagc cccgcggtgc
     6361 tcgaagaggt gctgtcgcgc gggatgctcg aggtgcttcc gctcacccac atccgcgggc
     6421 ggtcactgca cgactcgttc gtgatcgtcg acgaggcgca gtcgctggag cgcaacgtgc
     6481 tactgaccgt gctgtcccgg cttggcgccg gctcgagggt ggtgctcacc cacgacgtgg
     6541 cccaacgcga caacctccgg gtgggccgac acgacggcgt cggcgccgtc atcgagaagc
     6601 tcaagggtca cccgctgttc gcgcacatca cgctggtgcg cagcgagcgg tcgccgatcg
     6661 cggcgctggt caccgagatg ctcgaggaaa tcagccccgg cgcgttgccc tgagcgcgac
     6721 gcataagctg ctcaggtgcc gagacgacca gtcagcaggg ccaagccgta ctggctgacc
     6781 ctgtgtgcag cggcgggtgc cgtcgcagtg ctggtgttcg ggagtctgac cggccatgtg
     6841 aagctgacca gcgccgccga cgtcgactgc tcgaaggtca agtgcgtggc cttcacgttc
     6901 gacgacggtc ccggccccta caccgaccgg ctgctgcaga ttctcaacga caacgacgcg
     6961 aaggcgacgt tctttctgat cggcaacaag gttgcagcca acccggccgg cgcgaagcgg
     7021 atcgtcgacg ccggcatgga ggtgggcagc cacacctggg agcaccccaa catgacgacg
     7081 atcccgtccg acgcgattgc cggtcagctg tcgaaggcca gcgacgcgat cgcgtcagcg
     7141 accggccaaa ccccgaagtt gtggcgccct gccggaggat tgaccaacga cgaggtgaac
     7201 aacgttgcgg cgcagcaggg tctggcggcc atcctgtggg atgtcattcc gttcgactgg
     7261 atcaacgact cagacacggc ggcgacgcgc tacatgctga tgagccagat caagccgggc
     7321 tcagtggtgt tgttccacga cacctactcc agcaccgtcg acttggtgta ccagttcctg
     7381 ccggtgctga aggccaatgg gtatcacctg gtgacggtca gtcagctgct gggcccgcgc
     7441 gcgccgggca gtgtctacgg cagccgggat aacggaccgc ccgtcaacga ccttcacgac
     7501 gtcccacccg ccgacatccc gacgctaccc aacacgccgt cgcccaagcc gatgccgaac
     7561 ttcccgatca ccgacttgcc cgatcagaac tcgggcgggc ccaacaacgg cgcctgacct
     7621 cgtgaaaccg gggtttcgca gggtgtgctg gacgtcgtgc tgtccggctt cgcccgtgtt
     7681 atgaccaacc ggcgggtgtt cgatgagccg acgactacgc gtcaagtgtc ggaagcggtc
     7741 gacgcgctcc tcgcggcgcc tgcggtgatg cggctacgcg caggtgagcg gcactggttg
     7801 ttgttccgac aactcgtcga cgacgtcgac gcacgcggca atgacgtcgc cgacgcgtat
     7861 ctcgccgcct tcgcgcttga gaacaatgcc acctggctca gcgctgatcg tggcttcgcg
     7921 cggtttcacc gagtgcgctg gcgccacccg ctcggttgac caagtcgcta cgcctcttga
     7981 ggcttgacct tggccatcgc cagcacgtcg aggcgcttgt cgagttcttc ctccgacagc
     8041 ttgtcgccga tcagcccgcg gtcgatgacc gtctggcgga tcgtcttgtg ctctttgagc
     8101 gcctccttgg ccacggccgc cgcttcttcg tagccgatcg cggagttcag cggcgtcacg
     8161 atcgacggcg acgactccgc cagcgtgcgc aaccgatctt cgtgggcgat gagcccgtcg
     8221 atgcagcgct cggcgaacag acgcgacaca ttgg
//
LOCUS       DASWBF010000102        11190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_10616, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000102 DASWBF010000000
VERSION     DASWBF010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10616"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..472
                     /gene="greA"
                     /locus_tag="VF874_03575"
     CDS             2..472
                     /gene="greA"
                     /locus_tag="VF874_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX9175197.1"
                     /translation="MTQEAYDRLKAELDQLIANRPVIAAEINDRREEGDLRENGGYHA
                     ARDEQGQQEARIRQLQELLNNAKVGEAPKQSGVALPGSVVKVYYNGDESDTETFLIGT
                     RQEGVTDGKLEVYSPNSPLGGALIDAKVGETRTYMLPNGNTAKVTLVSAEPYHS"
     gene            509..1168
                     /locus_tag="VF874_03580"
     CDS             509..1168
                     /locus_tag="VF874_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HEX9175198.1"
                     /translation="MAQIAEDLLLLLLDNASNRPALDRDRREKVLAAAVLLDLAFACK
                     IRPSVDGEPVGRLLLLAGPEMGDPILDPALRRLQRRPLKPRAAVAKVRRGVEPALLRQ
                     LEQGGYIRPIQLRGNRSNGSPVWTMPDRSRVDQARSALLAALFDLRTPAPATAAVISL
                     LHAVDGLGALLSLNERGWRWVHSRAGDIAGGCWVDEAAASGLAQVNLAVTASAIRPAL
                     T"
     gene            complement(1160..2338)
                     /locus_tag="VF874_03585"
     CDS             complement(1160..2338)
                     /locus_tag="VF874_03585"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX9175199.1"
                     /translation="MSEKHRSRSAGDIERRGLATTAIHAGYRPDPATGAVNTPIYASS
                     TFAQDGVGDLRGGFEYSRTGNPTRTALEASLAAVEEAAFGRSFSSGMAATDCALRALL
                     RPGDHVVIPNDAYGGTFRLIDKVFTLWGLTYTAVPQSDLDAVRDAITDATRLIWVETP
                     TNPLLTVADITAIAELSRTRSVKVLVDNTFASPALQQPLTLGADVVLHSTTKYIGGHS
                     DVVGGALLTSDPELDEAFAFLQNGAGAVPGPFDAYLTLRGLKTLVVRMQRHTENASRV
                     AEFLADHPAVSVTLYPGLSSHPNHDVASRQMSGFGGMVSVRLRGGRQAALDLCSRTEV
                     FILAESLGGVESLIEHPGAMTHASTAGSLLEVPDDLVRLSVGIEDVVDLVGDLEQALR
                     "
     gene            2378..3550
                     /locus_tag="VF874_03590"
     CDS             2378..3550
                     /locus_tag="VF874_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9175200.1"
                     /translation="MGTAARIEDLLDVDSLLAAEEIELRNTVRRFGEQRLRPHIADWF
                     EAGDVPARELAADFGKLGLLGMHLKGYGCGGSTATAYGLVCQELEAIDSGLRSLVSVQ
                     GSLAMFAIYRHGSEEQREQWLPSMATGDAIGCFGLTESDFGSNPAGMRTNARRDGSDW
                     ILNGSKMWITNGTVADVAIVWARTDDGIAGFVVPTDTAGFSARTMSHKLSLRASITSE
                     LAFDDVRLPAEAKLPEARGLSGPLACLSEARFGIVFGAVGAARDCLEATLDYVNTREV
                     FDKPMSAYQLTQAKLADMAVELAKAQLLAVHLGHLKDAGKIRPDQVSVGKLNNVREAI
                     AIARQCRTLLGANGITLEYPVMRHANNLESVLTYEGTSEVHQLVIGEALTGVSAFR"
     gene            complement(3566..4267)
                     /locus_tag="VF874_03595"
     CDS             complement(3566..4267)
                     /locus_tag="VF874_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175201.1"
                     /translation="MGEAFSWAWNKFTKNAGPLIIASLVYGLVISIILGIFGGIAYAL
                     APAPVTTYESYDNGFSYSTSSGGLSVGSIIVMVIGLIVLILVGGAIQSAYTSSLLEIA
                     DGRQVDVGSFFKPRNVGSVIVATLIVGLAVLIGAAITCGILGIVIGFFTMFTTYALLD
                     RNLSPVDAIKASFEITKAKVGDSILAWLIGSLIAAVGVAVCYVGAIVTGPVAALFLTY
                     SYRRLSGGQVAPLTP"
     gene            complement(4766..5704)
                     /locus_tag="VF874_03600"
     CDS             complement(4766..5704)
                     /locus_tag="VF874_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175202.1"
                     /translation="MTEQPPGGYPPPQGGGYPPPQPPQRGGYPPQPPPGGGYPPPQPP
                     PGGGYPPLPPQSGGYPPPPGGYPPAGGPGYGGYGTQQPFDVGEGFKWAWNKFSKNAAP
                     LVIATLVYVLIAAVIEGIFIGLVIALSPEPDYASYDSGFSYSFSAFSLSGILVNAIGV
                     FVLLVVGYVIQSAYLSGVLDIANGQEVTVGSFFKPRNIGNVVVAGLIAGVLTAIGYAL
                     CYIPGIIVAFFLIFTIVALLDRNLAPVDAIKNSFETVKNNVGNALLAYLIMAVIGVVG
                     FFACCVGFLVAAPVSALFLVYTYRRLTGGQVAPLTP"
     gene            complement(5768..6091)
                     /locus_tag="VF874_03605"
     CDS             complement(5768..6091)
                     /locus_tag="VF874_03605"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX9175203.1"
                     /translation="MTEQPPGVYPPPPVQGSGYPPPAPGYSGASSTPGTNGLAIASFV
                     CSFLGGASGGIGAIVGIVLGVIALNQIKKSGQDGRGLALAGITVGAVWLVIGAIGFIA
                     YFSTT"
     gene            complement(6154..6498)
                     /locus_tag="VF874_03610"
     CDS             complement(6154..6498)
                     /locus_tag="VF874_03610"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX9175204.1"
                     /translation="MTEQPPGEYPPPPPEGYPPPPPPQGGGYPPPASGYSGATATGTN
                     GFAIASLVCSLVGWLCGGILSIIGIVLGVVALNQIKQTGQQGRGLALAGIIVGAVSLV
                     LGVIWAFMYFAR"
     gene            complement(6607..8013)
                     /locus_tag="VF874_03615"
     CDS             complement(6607..8013)
                     /locus_tag="VF874_03615"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX9175205.1"
                     /translation="MRIAQHISELIGNTPLVELHSVVPEGAGRVVAKVEYLSPGGSVK
                     DRIAVKMLDAAEASGELRSGGTIVEPTSGNTGVGLALIAQQRGYKCIFVCPDKVSEDK
                     QNVLRAYGAEVVVCPTAVPPDHPDSYYSVSDRLVEEIDGAWKPDQYSNPNGPASHYET
                     TGPEVWADTDGTVTHFVAGVGTGGTITGAGRYLKDVSDGRPEGPVRVIGADPEGSVYS
                     GGTGRPYLVEGVGEDFWPAAYDPHVPDEVIAVSDADSFEMTRRLAREEALLVGGSCGM
                     AVVAAVKVAEQAGPDALVVVLLPDGGRGYMSKIFNDDWMSSYGFLRSRLDGSVAQPTV
                     GDVLRGKSGALPDLVHTHPSETIRDAIGILREYGVSQMPVVGAEPPVMAGEVAGSVSE
                     RELLSAVFEGRAKLADAVGQHMSPPMPMIGTGELVGTAAKMLRDCDALMVVEGGKPAG
                     VITRQDLLGFLSGNGRQG"
     gene            complement(8036..9088)
                     /locus_tag="VF874_03620"
     CDS             complement(8036..9088)
                     /locus_tag="VF874_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175206.1"
                     /translation="MSAPSKVSGSPRAHIGAGRALKARKYPGSDGAPVEVVENGPSIA
                     GRLAWLGARLTIRPTLTVGSHIPFLPWPWGLVDFAARALLPIPGTVRATVKLSHCTAQ
                     LVRAKGVLPADGKRRIVLYMHGGAFLTCGANTHGRLTTMISHYADSPVLVLNYRMIPK
                     HSIRQAIDDCYDGYQWLRELGYEPDQIVLAGDSAGGYLALALAERLLDEGEEPAAMVS
                     LSPLMQLEMDPKKRHPNIRTDAMFPPKAFDALVDLIARAAARRIVDGEPEDVFEPLDH
                     VEPGLPPTLIHVSGSEVLLYDARLTARRLAAAGVPAEVRVWPGQMHVFQLCAPLVPEA
                     VRSLRQIGQYIREATE"
     gene            9278..10243
                     /locus_tag="VF874_03625"
     CDS             9278..10243
                     /locus_tag="VF874_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX9175207.1"
                     /translation="MGMRVPRRSTIGLAAVGMLASSGGAFVGARNFLSGQADQARSVI
                     PRNWEPPPRADGVYTAGGGPPERWSRGDPFDLHLMIFGDSTATGFGCSCADEVPGVLL
                     ARGLAEETGKRIRLSTKAISGATSKGLSGQVDAMFVAGPHPDAAVIMIGANDVTTLNG
                     VVPSARRLGSAVQRLRASGAVVVVGTCPDFGVITAIPQPLRWTARTRGLRLARAQAAA
                     VRTAGGIPVPLADLLAPEFLQAPELLFSEDKYHPSAAGYALAAEQLFPALCNALGEWT
                     GGPVPELPWVSRAAEARTLRSRLRQVARMWRRTTGVPAPVVVPAI"
     gene            10287..>11190
                     /locus_tag="VF874_03630"
     CDS             10287..>11190
                     /locus_tag="VF874_03630"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9175208.1"
                     /translation="MPEAVIVSTARSPIGRAGKGALVDMRPDDLAAQMVRAALDKVPA
                     LDPRDLDDLIMGCGQPGGEAGFNVGRAVAVQLGYDFLPGTTVNRYCSSSLQTTRMAFH
                     AIKAGEGSALVSAGVETMSRFPKGNSDGWPDTHNPRFVDAEERTAQAAAGADEWHDPR
                     EDGNLPDVYIAMGQTAENVVLLTGISREDQDHWGVRSQNRAEEAINNGFWAREITPVT
                     APDGTVVSKDDGPRAGTTLEALAGLKPVFRPDGTVTAGNCCALNDGAAAVIVMSDRKA
                     AELGITPLARIVSSGVSALSTEIMG"
BASE COUNT         1983 a         3683 c         3691 g         1833 t
ORIGIN      
        1 gttgacccag gaggcatacg accgcctcaa ggcggagctc gaccagctga ttgccaaccg
       61 cccggtcatc gccgccgaga tcaacgaccg gcgcgaggag ggcgacctgc gcgagaacgg
      121 cggctaccac gccgcccgcg acgagcaggg tcagcaggag gcgcgcatcc gtcagctcca
      181 agagctgctg aacaacgcca aagtgggcga ggcgcccaag cagtcgggcg ttgcgttgcc
      241 cggctcggtc gtcaaggtct actacaacgg cgacgagagc gacaccgaaa ccttcctcat
      301 cggcacccgc caagaaggcg tgaccgacgg caagcttgag gtgtactcgc cgaactctcc
      361 gctcggaggt gcgctgatcg acgcgaaggt cggcgagacc cgcacctaca tgctgcccaa
      421 cggcaacacc gcgaaggtga cgctggtcag cgcggagccg taccactcgt aacgagggtc
      481 ggcagcctga gcggttagag tcccgctcat cgcgcagatc gctgaagacc tgcttctgct
      541 gctgctcgac aacgcgtcca accgccccgc actcgaccgc gaccgccgcg agaaggtgct
      601 cgccgccgcg gtgctgttgg acctcgcgtt cgcatgcaag attcgcccgt ctgtcgacgg
      661 cgagccggtc ggtcggctgc tgttgctcgc cggaccggaa atgggtgacc cgatccttga
      721 tcccgcgtta cgacggctgc agcggcgccc gctgaaaccc agggcggcgg tggccaaggt
      781 gcggcgcggt gtggagccgg cgctactgcg ccagctggag caaggcggtt acattcgtcc
      841 aattcaattg cgcggcaatc gatccaacgg ctctccggtg tggacgatgc cggaccgttc
      901 gcgggtggac caggcgcgct cggcgctgct cgctgcgctg ttcgacctgc gcactccggc
      961 accggcgacc gcggcggtga tctcgcttct gcacgccgtg gacggactgg gggcgctgct
     1021 gagcctcaac gagcggggat ggcgatgggt gcactcccgc gcgggggaca tcgccggcgg
     1081 atgttgggtc gacgaagccg ctgcttcagg actggcccag gtgaacctgg cggtgacggc
     1141 gtcggcgatc agaccggcgc taacgtagcg cctgctcgag atcgccgacc agatcgacga
     1201 cgtcttcgat gccgaccgac aggcggacca gatcgtcagg cacctcgagc agcgacccag
     1261 cggtcgacgc gtgcgtcatc gcaccagggt gctcgatcaa cgactcgacg ccaccgagcg
     1321 actcggcgag aatgaaaacc tctgtgcgag aacataaatc cagggcagcc tgtcggcccc
     1381 ctcgcagtcg aaccgacacc ataccgccga atccgctcat ctgccgggac gcgacgtcat
     1441 ggttggggtg gctcgacaac ccggggtaca gcgtgacgct taccgctggg tggtcggcga
     1501 ggaattccgc aacgcggctt gcgttttcgg tatgccgctg catccgcacc actaacgtct
     1561 tcaacccgcg cagggtcaga taagcgtcga acggtcctgg cacagcgccg gccccgttct
     1621 gcaggaaggc gaacgcttcg tcgagctcgg ggtcgctggt cagcagcgca ccgcccacca
     1681 cgtcggagtg accgccgatg tacttcgtgg ttgagtgcaa cacgacgtcg gcgcccagag
     1741 tcagcggttg ctgcagcgcc ggcgatgcaa acgtgttgtc taccaatact ttcacggatc
     1801 gcgtccgaga aagctcggcg atcgcggtga tatcggctac cgtcagcagc ggattagtcg
     1861 gcgtctcaac ccatatcaac ctcgtcgcat cggtaatcgc gtcgcgtacc gcgtcgagat
     1921 ccgactgcgg aaccgcggta tacgtcagcc cccacagcgt gaacaccttg tcgatgagcc
     1981 gaaatgttcc gccgtacgcg tcgttgggaa tcacgacatg gtcgccgggc cgcagcagcg
     2041 cgcgcagtgc gcaatcggtg gccgccatcc ccgagctgaa ggagcggccg aacgccgcct
     2101 cctcgaccgc cgccagcgac gcttccagcg ccgtgcgggt tgggttgccg gtgcgggagt
     2161 actcgaaccc gccgcgcagg tcgcctacgc cgtcctgggc gaacgtcgag ctggcgtaga
     2221 tgggtgtgtt gacggcgccg gtcgccgggt cgggccggta gccggcgtga atggccgtcg
     2281 tcgccaggcc gcgccgctcg atgtcgccgg ccgaccggct ccggtgcttc tcgctcatgg
     2341 tgtccgagcc tattgccccc ccgtgtagac atagggcatg ggtactgcgg ccaggatcga
     2401 agacctgctt gacgtcgact cgctgctggc ggccgaagag atcgagctgc gcaacaccgt
     2461 gcgccggttc ggcgagcagc gcctgcgccc gcatatcgcc gactggttcg aggccggcga
     2521 cgtgcccgcc cgcgagctcg ccgccgactt cggcaagctc ggtttgctcg gcatgcacct
     2581 gaagggctac ggctgcggcg gctcgactgc caccgcctac gggctggtgt gccaggagct
     2641 ggaggcaatc gacagcgggt tgcgcagcct ggtgtcggtg caggggtcgt tggcgatgtt
     2701 cgcgatctac cggcacggct ccgaagagca gcgcgagcag tggctgccgt cgatggccac
     2761 cggcgatgcg atcgggtgct tcggcctcac cgagtccgac ttcgggtcga atcccgccgg
     2821 gatgcgcacg aacgcacgtc gcgacggcag cgactggatt ctcaatggct caaagatgtg
     2881 gatcaccaac ggcacggtcg ctgatgtcgc gatcgtctgg gctcggacgg acgacggcat
     2941 cgcgggcttc gtcgtgccga ccgatacggc gggcttttcc gcgcgcacga tgtcgcacaa
     3001 gttgtccctg cgcgcgtcga tcacctccga gctggcattc gacgacgtcc gactgcctgc
     3061 agaggcgaaa ctgcctgagg cgcgcggatt gtcgggcccg ctggcatgcc tgtcggaggc
     3121 ccggttcggc attgtattcg gcgctgtcgg cgcggcccgg gactgcctgg aggcaacgct
     3181 cgactacgtg aatacgcgcg aggtgttcga caaaccgatg tccgcttatc agctgacgca
     3241 ggccaagctc gccgacatgg cggtcgaact ggccaaggcg cagctgctcg ctgtgcacct
     3301 cggccacctc aaagatgccg gcaagattcg gcccgaccag gtgagcgtcg gaaagctcaa
     3361 caacgtccgc gaagcgatcg cgatcgcacg gcagtgccga acactgttgg gcgccaacgg
     3421 catcacactg gagtacccgg taatgcgtca tgccaacaac ctcgagtcgg tgctgaccta
     3481 cgaaggcacc tccgaggtgc accagctggt gatcggcgaa gcgctgaccg gggtttcggc
     3541 gttccggtag gcggtccctc gcgcgctacg gggtcagcgg cgcgacctga ccaccgctga
     3601 ggcggcggta gctgtacgtc aggaacaagg ccgccacggg cccggtgacg atggccccga
     3661 cgtagcagac cgccacaccg acggccgcga tcaaactacc gattagccac gcgagaatgg
     3721 agtcgcccac tttggccttc gttatctcga agctggcctt gatcgcgtcg acgggcgaca
     3781 ggttgcggtc gagcagcgcg taggtcgtga acatcgtgaa gaagccgatg acgatgccca
     3841 ggatcccgca ggttatcgcg gcgccgatga gaaccgcgag gcccacgatg agggtcgcga
     3901 caatgacgct gccgacgttg cgtggcttga agaatgaccc gacatccacc tggcgcccgt
     3961 cagcgatttc gagcaggctg ctcgtgtacg cggactggat cgcaccgccg acaaggatca
     4021 gcacgatgag gccgatgacc atcacgatga tgctgccgac actgaggccc cctgaactcg
     4081 ttgagtacga gaagccgttg tcgtacgact cataggtggt gacgggtgca ggggccagcg
     4141 cgtaagcgat gcccccgaag atccccaaga tgatcgatat cacgagcccg tacaccaggc
     4201 tggcgataat cagagggccg gcgttcttgg tgaacttgtt ccacgcccat gagaaggcct
     4261 cgcccacgct gtacggctgt tcggcgctgt aaccgccata gcccgggcct cccgcggacg
     4321 ccgggtaacc gccctgcgga ggcggtgttt gataaccgcc accctgcggt ggtggaggcg
     4381 gcgggtaaga gcctcctccg gacggcggct gaggtggtgg gtaaccgcca ccctgcggtg
     4441 gtggaggcgg cgggtaagag ccagggggcg ggccgggggg ttgttcagtc atcgtcgtcc
     4501 tccacgtgag ttctcaaccg gcgtcaaacg ctgcatacgt tagccgaggc acacgtggga
     4561 atctatgcct atcgggcaat tcctgccgct gcaggcgaaa aggcaagcga gaacgcaagt
     4621 cacgcagcac aaccggctcc gacgcccctg gttgcattgc cgccgtctcg ctcggcgcga
     4681 aaaccacagt gggaagccat aattgacgcc gtccccgaca gggcccagac ctcgcgggcc
     4741 ctgtcgggga cggcagtacg tgcggctacg gggtcagcgg cgcgacctga ccgccagtga
     4801 ggcggcggta ggtgtacacc aggaaaagtg cgctcaccgg cgcggcgaca aggaacccga
     4861 cgcagcaagc aaagaaacct acaaccccga tgacggccat gatcaggtac gccagaagag
     4921 catttccgac gttgttcttg accgtctcga aactgttctt gatcgcgtcg acgggcgcca
     4981 aattgcgatc gagcaacgcg acgatcgtga atatcaggaa gaaggccacg ataattccgg
     5041 ggatgtagca cagtgcgtag ccgattgccg tcagcacgcc cgcgatcagg cctgcgacga
     5101 cgacgttgcc gatgttgcgg ggcttgaaga aggacccgac ggtcacttct tgcccgttgg
     5161 cgatgtccag cacgccgctg aggtacgcgg actgaatcac gtaacccacc accagcagca
     5221 cgaagacgcc gatggcattc acgaggatgc cgctcagact gaatgcacta aaggagtacg
     5281 agaagccgct gtcgtacgac gcgtagtcgg gctccggaga caacgcgatc accagaccga
     5341 taaagattcc ttcgatcacc gctgcgatca ggacgtacac cagggttgcg atgaccaacg
     5401 gggcggcgtt cttgctgaac ttgttccacg cccacttgaa gccttcgccg acgtcgaacg
     5461 gctgctgggt gccgtagccg ccgtagccgg gtccgccggc gggtgggtag ccaccagggg
     5521 gcggcgggta gccgccgctc tgcggcggca gtggcggata accgccaccc ggcggcggtt
     5581 gcggcggcgg atagccacca cccggcggcg gttgcggcgg gtaaccgcca cgctgtggag
     5641 gctgaggcgg cgggtagcca ccaccctgtg gaggcgggta accgccggga ggttgttcgg
     5701 tcatgattcc cttctagatc gaattacctt acctgcgttc agatttcgac ggatttagtt
     5761 ggccatgtca cgttgtggag aagtaggcaa tgaaaccgat ggcgccaatc accaaccaaa
     5821 cggcacccac ggtgatgccg gccaaagcta ggccgcgacc gtcctgaccg gacttcttga
     5881 tctggttcag cgcgatgacg ccaagcacaa tcccgacgat agctccgatg ccgccgctgg
     5941 cgccgcccaa aaaggagcaa acgaacgacg cgatagccaa gccattggtc ccaggagttg
     6001 aactcgcacc cgaatagccc ggggccggtg gcgggtagcc gctaccctgc acgggcggcg
     6061 gcgggtagac gccaggcggc tgttcggtca tgatcccctt ctagatcgaa ttgctctgcg
     6121 tgcgttggga ttcgggtgga ctcgttcgca agatcaccgg gcgaagtaca tgaaagccca
     6181 gatcactccc agcactagtg aaaccgcacc cacgatgatg cccgccaacg ccagtccgcg
     6241 gccctgctga ccggtttgct tgatctggtt caaggcgaca acgccaagga cgatcccgat
     6301 gatggacagg atgccgccgc acaaccagcc gacgagagag cacaccaacg acgcgatcgc
     6361 gaacccattg gtccccgtcg ccgtagcacc cgagtagccc gaggcgggcg gtgggtaacc
     6421 gccgccctgc ggtggcggtg gtggcggata accttcgggc ggtggcggcg ggtattcacc
     6481 gggaggctgt tcagtcataa cgccttctca ctcaggtgat ctgcaagctg ggtgaagcgg
     6541 tgctcaccat acccgagaag gcggtccaga atccggtaag tgacgaatcg gcgcggacgt
     6601 cggcggctag ccctgacgac cattacccga caggaatccg agcaggtcct gcctcgtgat
     6661 gaccccggcg ggcttgcctc cctcgaccac catcagtgcg tcgcagtcgc gcaacatctt
     6721 cgccgcggtt cccacgagtt cgcccgtccc gatcattggc atcggcgggc tcatgtgctg
     6781 cccgaccgcg tcggcaagtt tggcgcgacc ctcgaacacc gcggagagca gttcgcgttc
     6841 ggacacgctg cccgcgactt cccctgccat caccgggggt tcggctccca cgaccggcat
     6901 ctgcgacacc ccgtactcgc gcagaattcc gatcgcgtcg cgaatcgtct cggatggatg
     6961 ggtgtgcacc agatcgggca gcgctccgga cttgccacgc aacacatctc cgacggtcgg
     7021 ctgcgccacg gagccgtcga gccggctgcg caggaatccg tacgacgaca tccaatcgtc
     7081 gttgaagatc ttcgacatgt agccacgtcc cccgtcgggc aacagcacga ccaccaaagc
     7141 gtcaggtccg gcctgctcgg caaccttcac ggccgcaacc accgccatcc cgcacgaacc
     7201 gcccaccagc aatgcctctt cgcgggcgag ccgcctggtc atctcgaatg aatcggcatc
     7261 cgacacggcg atgacttcgt cgggaacatg cgggtcatag gcggccggcc agaagtcctc
     7321 gccgacgccc tcgaccagat acggcctgcc ggtcccgccc gagtagacgg acccttcggg
     7381 gtccgcaccg atgacgcgca ctgggccttc tggtctgccg tccgacacgt ctttcaggta
     7441 ccgcccggca ccggtgatcg tcccgccggt acccacgccg gcgacgaaat gggtcacggt
     7501 gccgtccgtg tccgcccaca cctccggtcc ggtggtctcg taatggctgg ccggcccgtt
     7561 cgggttcgag tactggtccg gcttccacgc gccgtcgatc tcttcgacca atcggtcgga
     7621 aacgctgtag tagctgtcgg ggtgatccgg cggcaccgcc gtcgggcaga cgacgacctc
     7681 tgcgccgtat gcccgcagca cgttctgctt atcctcgctg accttgtccg ggcagacgaa
     7741 gatgcacttg tagccgcgct gctgagcgat cagcgccagg ccgacgccgg tattgcccga
     7801 cgtgggctcg acaatggttc cgccggaccg caactcgccg ctggcctcgg cggcatcgag
     7861 catcttgacg gcgatccggt ctttcacgct gccgccggga ctgaggtact cgaccttcgc
     7921 aacgaccctg cccgccccct ccgggacgac cgagtgcaac tcgaccagag gggtgttgcc
     7981 gatcagctcg ctgatgtgct gggcaatccg catctgtcca tgatcgcagc cgcggctact
     8041 ccgtggcttc gcggatgtac tgaccgatct gccgtagcga ccggaccgcc tccggtacca
     8101 gcggcgcgca caactggaac acgtgcatct gaccgggcca gacccgcacc tcggcgggaa
     8161 cgccggccgc ggccagtcgg cgcgcggtca agcgggcgtc gtacagcagc acctcggagc
     8221 ccgagacatg aatcagcgtg ggcggcaagc ccggttcgac gtgatccagc ggctcgaaga
     8281 cgtcttcagg ctcgccgtcg acgatccgcc gggcggctgc acgcgcgatt aggtcgacca
     8341 acgcatcaaa agccttgggc gggaacatcg catcggtgcg gatgttcgga tgcctcttct
     8401 tcgggtccat ctccagctgc atcagcggcg agagcgacac cattgccgcc ggctcctcac
     8461 cctcgtcgag cagccgttcc gcgagcgcca gcgcgaggta gccgccggcc gaatctcccg
     8521 ccagcacaat ctgatccggc tcatagccca gttcgcgcag ccactgataa ccgtcgtagc
     8581 agtcgtcgat cgcctggcgg atcgaatgtt tcgggatcat ccggtaattg agcaccaaga
     8641 cgggcgagtc ggcgtagtga gaaatcatcg tcgtgagccg accatgcgtg ttcgctccgc
     8701 acgtcaggaa cgcgccgccg tgcatgtaca gaacgatgcg acgcttgccg tcggccggga
     8761 gcacgccctt ggcccggacg agctgcgcgg tgcagtggct cagcttgacg gtcgctcgga
     8821 cagtgcccgg gatgggcagc agcgcccgtg cggcaaagtc gacgaggccc catggccagg
     8881 gcaggaatgg gatgtggctt ccaactgtca gtgtcggtcg aattgttaac cgggcaccca
     8941 gccaggccag ccgcccagcg atgctgggcc cgttttcgac cacctcgacc ggagcgccgt
     9001 cactgcccgg gtacttgcgg gctttaaggg cacgacctgc accgatatgt gcacggggcg
     9061 aaccagagac cttacttggt gcgctcatgt cacactccta cgccgttgta gggccggatg
     9121 ccgacgggtc gctccgtcaa ttcccggtaa cttagcttga catctgtaac cagttcaacg
     9181 attcacaaaa gtgttttgtt accggatccg atgttggccc gtgatcagac tgagtttcgt
     9241 ggccggattc ctgcgtcttc gcacctagac ttgacgcgtg ggcatgcgcg tgccgcgtcg
     9301 gtcgaccatc gggttggcgg ccgtggggat gctggcatcc tccggcggcg cattcgtcgg
     9361 cgcacgcaac ttcctgagcg gacaggcgga tcaggctcgc agcgtgattc cgaggaactg
     9421 ggagcctccg ccccgcgcgg acggcgtcta tacggccggc ggcggtccgc ccgagcgctg
     9481 gtctcggggt gatcccttcg acttgcatct catgattttc ggcgactcca ccgcaacggg
     9541 tttcggctgc agctgcgccg acgaagtgcc cggcgtgctg ttggcccgag gcctcgccga
     9601 ggagaccggt aaacgaattc ggttgagcac caaggctatt tcgggtgcga cgtcgaaggg
     9661 gttgtcgggg caagtcgacg cgatgttcgt cgccggtccg caccccgatg ccgcggtcat
     9721 catgatcggc gccaacgacg tcaccacgct caacggagtc gtgccgtcgg cccgtcgtct
     9781 cggttcggct gtgcagcgcc ttcgggcaag cggcgcggtc gtcgtcgtcg gcacttgccc
     9841 cgacttcggg gtgatcaccg cgattccaca gcccctgcgg tggaccgcgc gtactcgagg
     9901 actgcggttg gcacgtgcgc aggccgccgc cgtccgcacc gcaggcggca tcccggtgcc
     9961 gctggccgac ctgctcgcac ccgagttcct ccaggcccct gagctgctgt tctccgaaga
    10021 caagtaccac ccgtcggcgg cgggttatgc actggcggcc gagcaacttt tcccggcgct
    10081 ctgcaatgcg ctgggcgagt ggaccggcgg gccagtgcca gagctgccgt gggtgtcgcg
    10141 tgcggcggaa gcgagaactc ttcggagccg gctgcgccag gtggccagga tgtggcggcg
    10201 gaccacgggc gtcccggcac ccgtcgtggt gcccgcgatc tgacccagac taggttcgtc
    10261 gtagttccgt cactacagga ggcgtcatgc ctgaagccgt cattgtctcg accgcccgtt
    10321 caccgattgg ccgtgccggc aagggcgcgc tggtcgacat gcgtcccgac gacctggcag
    10381 cgcagatggt gcgcgccgcc ctggacaagg tgcccgcgtt ggatccgcgc gacttggacg
    10441 acctgatcat gggctgcggc caaccgggtg gcgaggcggg cttcaacgtc ggccgcgcgg
    10501 tggccgttca gctgggatac gacttcctgc cgggcaccac cgtgaaccgc tactgctcgt
    10561 cgtcgttgca gaccacccgc atggcgtttc acgcgatcaa ggcaggcgag ggcagcgcgc
    10621 tcgtctccgc gggtgtcgag accatgtcgc gcttccccaa aggcaactca gacggctggc
    10681 cggacacgca caaccccagg ttcgtcgacg cggaggagcg caccgcgcaa gcggccgcgg
    10741 gtgcggatga atggcacgac ccgcgcgagg acggcaacct gccagacgtc tacatcgcaa
    10801 tgggccagac cgcggaaaac gttgtgctgc ttaccggtat cagccgcgag gaccaggacc
    10861 actggggcgt gcgcagccag aaccgcgccg aggaggccat caacaacggc ttctgggcgc
    10921 gggagatcac ccccgtcacc gcgccggacg gcaccgtcgt cagcaaggac gacggcccgc
    10981 gggccggcac gacgctcgag gcgctggccg ggctgaagcc ggttttccgg cctgacggca
    11041 cggtcactgc gggcaactgc tgcgcgctga acgacggcgc cgcggcggtg atcgtgatga
    11101 gcgaccgcaa ggccgccgag ctcgggatca cgccgctggc caggatcgtc tcctccgggg
    11161 tgagcgcgct gtcaaccgag atcatgggcc
//
LOCUS       DASWBF010000103         4861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1124703,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000103 DASWBF010000000
VERSION     DASWBF010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124703"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VF874_03635"
     CDS             complement(<1..496)
                     /locus_tag="VF874_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9175209.1"
                     /translation="MASLNLSVDEVLTTTRSVRKRLDFEKPVPRGVLLECLELAIQAP
                     TGSNQQGWQWVFVEDTEKKQVLADIYRRNFENYRRTPPPRYADGDIRADQRDRVVSSA
                     MYLAEHMHEAPVLLVPCLEGRVDMAPDVSASFWGSLLPAAWSYMLALRSRGLGSAWTT
                     LHLLG"
     gene            complement(590..3886)
                     /locus_tag="VF874_03640"
     CDS             complement(590..3886)
                     /locus_tag="VF874_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX9175210.1"
                     /translation="MGWFTGPSWGEIERVLNGKPRRAGMPLESPVDGGDSPAWSRKRG
                     AYLKPEMQPATSRIPYAELHVHSAYSFLDGASTPEELVEEAVRLDLRAIALTDHDGLY
                     GVVRFAEAAKELGMRTVFGAELSLANKPRTEDADPPGPHLLVLARGPEGYRRLSRQLS
                     KAHLAGGEKGKPLYDFDALTEAAGGHWHILTGCRKGHVRQALTAEGPEAAERALADLV
                     DRFGQRRVSIELTHHGQPLDDEHNAALAALAPKFGVDVVATTAAHFAEPSRARLAMAM
                     GAIRARHSLDEAAGYLAPLGGSHLRSGAEMARLFPSEVVTAAADLGEQCAFALELIAP
                     QLPPFDVPDGHTEHSWLRHLTMEGARQRYGPPDRAQRAYAQVEHELKTIQALKFPGYF
                     LVVHDITRFCRENGILCQGRGSAANSAVCYALGVTAVDPVANELLFERFLSPERDGPP
                     DIDIDIESGLREKAIQYVYDRYGRDYAAQVANVITYRGRSAVRDMARALGFSQGQQDA
                     WSKQISRWNGVCPNENARFPDVEDIPEPVIDLALQISNLPRHMGIHSGGMVICDRPIA
                     DVCPVEWARMENRSVLQWDKDDCAAIGLVKFDMLGLGMLSALHYAIDLVAEHKGIDID
                     LAKLDLSEPAVYEMLQKADSVGVFQVESRAQMATLPRLKPRIFYDLVVEVALIRPGPI
                     QGGSVHPYIRRRNGEDPVTYDHPSMAPALRKTLGVPLFQEQLMQLAVDCAGFTAAEAD
                     QLRRAMGSKRSTEKMQRLKGRFYDGMRERHGITGDVADRIYEKLEAFSNFGFPESHSL
                     SFASLVFYSSWFKLHHPAAFCAALLRAQPMGFYSPQSLVADARRHGVLVHNPDVNASL
                     AHATLEDHGTEVRLGLGSVRHIGDELAEKLVDERKANGPFASLLDLTNRIQLTVPQTE
                     ALATADALGCFDISRREALWAAGAAATERPDRLPGVGSSSHIPALPGMTEIELASADV
                     WATGISPDSYPTQFLRDDLDAIGVIPADQLLKVPDGTRVLIAGAVTHRQRPGTARGVT
                     FLNLEDETGMVNVLCTPGVWARHRRVAQTAPAMLIRGQLQNATGAVTVVAERLGHIEL
                     AIGSRSRDFR"
     gene            4044..4742
                     /locus_tag="VF874_03645"
     CDS             4044..4742
                     /locus_tag="VF874_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX9175211.1"
                     /translation="MTQTTSAKPPVVDQQTWRSALDELRRREKAATRELDAIAAQRRR
                     LPMVEMPDYTLIGKDGPVRLVDVFEGRSQLIVYNHMWEDGAEWQCGGCTGFTSQFTRL
                     DFLDNYDARFVIVTNGPIEEALSYRDKVGNKMDWYSSSESPFAADVDAPPGAGFAVNV
                     FLRDGDTVYRTWHTNGRGTEQLSHSFGLIDILPWGRQEEWLDSPEGWPSRPTYSGWLD
                     SPDIARIYGQDSNS"
     gene            4739..>4861
                     /locus_tag="VF874_03650"
     CDS             4739..>4861
                     /locus_tag="VF874_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX9175212.1"
                     /translation="MTDERYVVTRTIAATPGEIFAILSDPSRHRDTEPGDWVRDA"
BASE COUNT          784 a         1622 c         1640 g          815 t
ORIGIN      
        1 cgccgagcag atgcaacgtc gtccacgccg accccagccc gcgtgaccgc agcgcgagca
       61 tgtagctcca ggctgcggga agcagcgacc cccagaacga cgcgctcacg tccggtgcca
      121 tgtcgacgcg gccttccagg cacggcacca gcagcacggg cgcttcgtgc atgtgctcgg
      181 ccagatacat cgccgacgac accacccggt ccctctggtc ggcgcggatg tcaccgtcgg
      241 cgtaccgcgg cggcggcgtc cggcggtagt tctcgaaatt tctccggtag atgtcggcaa
      301 ggacctgctt tttttccgtg tcttcgacga acacccactg ccagccctgt tggttcgatc
      361 ccgtcggcgc ctggatcgca agctcgaggc attccagcaa caccccccgt ggcacgggct
      421 tctcgaagtc caggcgcttg cggaccgagc gggtggtggt caggacttcg tcgacggaca
      481 gattcaggct tgccatgcgt ggagactact tttctgccgc gagcgtgcgc gtttgtaccc
      541 cgacaccaac gccaggggta caaacatgca cgctcgcgcg gaacgagagt cagcggaagt
      601 cgcgggagcg ggagccgatg gccagttcga tgtggcccag ccgttcggcc accacggtga
      661 cggctccggt ggcgttctgc agctgaccgc ggatcagcat ggccggggcg gtctgggcga
      721 ctcggcggtg acgggcccac acgccgggcg tgcacagcac gttgaccatc ccggtctcgt
      781 cctcgaggtt caggaacgtc acgccgcgcg cggtgccggg acgctgacga tgggtcaccg
      841 cacccgcgat caggacccga gtgccgtcgg gcactttcaa gagctggtct gcggggatca
      901 cgccgatcgc gtcgaggtcg tcgcgcagga actgggtcgg gtagctgtcc ggtgagatgc
      961 cggtggccca tacgtcggcc gaggccagct cgatctccgt catgccgggc agcgccggga
     1021 tgtgcgacga cgatccgacg cccggcagcc ggtcgggcct ttcggtggcg gccgcccccg
     1081 ccgcccacag cgcctcgcgg cggctgatgt cgaagcaccc gagcgcatcc gccgtggcca
     1141 gcgcctcggt ctgcggcacc gtcagctgaa tccggttggt caggtcgagc agagaggcaa
     1201 aggggccgtt ggcttttcgc tcatcgacca gcttttcggc cagctcgtcg ccgatgtggc
     1261 ggacgctgcc caaccccagc cgcacctcgg tgccgtgatc ttccagcgtg gcgtgcgcga
     1321 ggctggcgtt gacgtccggg ttgtgcacga gaacgccgtg gcgccgtgcg tcggcgacca
     1381 gtgactgcgg ggagtagaag cccatcggct gcgcccgcag cagcgcggcg cagaacgccg
     1441 ccgggtggtg cagcttgaac caggacgagt agaacaccag cgacgcgaag gacagcgaat
     1501 ggctttccgg gaagccgaaa ttggagaacg cttccagctt ttcgtagatc cggtctgcca
     1561 cgtcgccggt gatgccgtgt cgttcgcgca tgccgtcgta gaaccggccc tttagccgtt
     1621 gcatcttctc ggtggaccgc ttggatccca tcgcgcggcg cagttggtcg gcttcggctg
     1681 cagtgaagcc ggcgcaatcg accgcgagtt gcatcagttg ctcctgaaac agcggcacgc
     1741 ccaatgtctt tcgcagcgcc ggcgccatcg acgggtggtc gtaggtaacc gggtcttcgc
     1801 cgttgcgccg ccggatgtag gggtgcacgg agccgccctg aatcggcccg ggccggatca
     1861 gtgccacctc gacgacgagg tcgtagaaga tgcgtggctt cagccgaggc aaggtggcca
     1921 tctgcgcacg ggactccacc tggaagacgc cgacggaatc cgccttctgc agcatctcgt
     1981 agacggcggg ctccgacagg tcgagtttcg cgaggtcgat gtcgatgccc ttgtgctcgg
     2041 cgaccaggtc gatggcgtag tgcagcgccg acagcatgcc gaggccgagc atgtcgaact
     2101 tcaccaatcc gattgcggcg cagtcgtctt tgtcccactg cagcacactg cggttctcca
     2161 tccgggccca ctccaccggg cacacgtcgg cgatcgggcg gtcgcagatc accatgccgc
     2221 ccgaatggat gcccatgtgc cggggcagat tgctgatctg cagcgccagg tcgatcaccg
     2281 gctcggggat gtcctcgacg tcggggaacc gtgcattttc atttgggcag actccgttcc
     2341 agcggctgat ctgcttgctc caggcatcct gctgaccttg cgaaaagcca agcgcgcgag
     2401 ccatgtcgcg tactgcgctg cggccgcggt aggtgatgac gttggcgacc tgtgcggcgt
     2461 agtcgcggcc gtagcggtca tagacgtact gaatggcctt ctcgcgcagg ccggattcga
     2521 tgtcgatgtc gatatcaggc ggcccgtcgc gttcgggcga caggaaccgt tcgaacagca
     2581 gctcattggc taccgggtcg acggcggtga cgcccagggc atagcacacc gccgagttgg
     2641 ccgccgatcc cctgccctgg cacaagatgc cgttctcccg gcagaaccgc gtgatgtcgt
     2701 gcaccaccag gaagtagccc ggaaacttca gcgcctgaat ggttttcagc tcgtgctcga
     2761 cctgggcgta ggcccgttgc gcacggtccg gcgggccgta tcgttggcgg gcgccttcca
     2821 tcgtcaggtg acgcagccag ctgtgctcgg tgtggccgtc gggaacgtcg aacggcggca
     2881 gttgcggtgc gatcagttcg agcgcgaatg cgcactgctc gccgaggtcg gcggctgccg
     2941 tcaccacctc ggacgggaac aggcgcgcca tctcggcgcc ggagcgcagg tgcgaaccac
     3001 ccagcggcgc aaggtatccg gcggcctcgt ccagggagtg gcgcgcgcgg atggcgccca
     3061 tcgccatcgc cagccgggct cgtgacggct cggcgaagtg cgcggcggtg gtggcgacga
     3121 cgtccacgcc gaacttcggt gccagtgcgg ccagtgccgc gttgtgttcg tcgtcaagcg
     3181 gttgaccgtg gtgagtgagc tcgatactga cccgcctctg cccgaaccgg tccaccaggt
     3241 cggccagcgc cctctcggca gcctcggggc cttctgcggt aagcgcttgg cgcacatggc
     3301 ctttgcggca gcccgtcaag atgtgccagt gtccgccggc ggcctcggtg agcgcgtcga
     3361 aatcgtagag cggcttgcct ttctcgccgc cggccagatg cgctttggac agttgccggg
     3421 acagccttcg gtagccttcg ggaccgcggg ccagcaccag caggtgcggt cccggcggat
     3481 cggcgtcctc ggtgcggggc ttgttggcca gcgacagttc ggcgccgaac accgtgcgca
     3541 tgcccagctc cttggcggcc tcggcgaagc gcacgacgcc gtagaggccg tcgtgatcgg
     3601 tcagcgcgat cgcacgcaga tcgagccgga cggcctcctc gacgagttct tccggcgtgc
     3661 tggcgccgtc gaggaagctg tacgcggagt ggacgtgaag ctcggcgtag gggatccggg
     3721 acgtggccgg ctgcatctcg ggcttgaggt aggcaccgcg cttgcgcgac caggccgggc
     3781 tgtcgccgcc gtcgactggc gactccagcg gcatgccggc gcggcggggc ttgccgttga
     3841 gcacccgctc gatctcgccc cagctcggcc cggtgaacca gcccacccag acatgttatc
     3901 gaacaattgt tcgatcagcg aaaccgaatt ccaggttgtg atttcgcgcg atccacgacc
     3961 atggcttcat tctcggcgtc cagacgatga atatcgggcg cccgtccggt ctatacgaca
     4021 agcgaccgat gaaggagacc cgaatgaccc agaccacatc cgcgaagccc ccggtcgtcg
     4081 accagcagac ctggcgcagc gctctcgacg agctgcggcg gcgggagaag gccgccaccc
     4141 gcgagctgga cgccatcgcc gcacaacgcc gccgactgcc gatggtcgag atgcccgact
     4201 acacgctgat cggcaaggac ggccccgtcc ggctggtcga cgtgttcgag ggacgatcgc
     4261 agctcatcgt ctacaaccac atgtgggagg acggcgcgga gtggcaatgc ggcggctgca
     4321 ccggcttcac gtctcagttc acccggctgg acttcctcga caactacgac gcccgcttcg
     4381 tgatcgtcac caacgggccg atcgaggagg ccctgtccta ccgcgacaag gtcggcaaca
     4441 agatggactg gtactcgtcg tcggagagcc cgttcgccgc cgacgtcgac gcgccgcccg
     4501 gcgccgggtt cgccgtcaac gtgttcctgc gtgacggcga cacggtgtat cgcacctggc
     4561 acaccaacgg ccgcggcacc gaacagctca gccactcgtt cggtctcatc gacatcctgc
     4621 cgtggggccg acaggaggaa tggctcgact cacccgaggg ctggccgtcg aggccgacct
     4681 attccggctg gctggattca cccgacatcg cccggatcta cggacaagac tccaactcat
     4741 gaccgatgag cgttacgtcg tgacccgcac catcgccgcc acaccgggcg agatcttcgc
     4801 gatcctttcc gacccgtcgc ggcatcggga caccgaaccg ggcgactggg tgcgcgacgc
     4861 g
//
LOCUS       DASWBF010000104         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_405874,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000104 DASWBF010000000
VERSION     DASWBF010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405874"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VF874_03655"
     CDS             <1..152
                     /locus_tag="VF874_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9175213.1"
                     /translation="ADIDGIHVNGVDMIHWNDDEKIVEFKVMLRPLKALQAVIPAMAE
                     LLQNP"
     gene            149..640
                     /locus_tag="VF874_03660"
     CDS             149..640
                     /locus_tag="VF874_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9175214.1"
                     /translation="MKLLLISDTHIPKRARDLPSRVWDEVERADVVLHAGDWVDVSLL
                     DALGERAPRLIGCWGNNDGPALRARLPEEADATLEGIRFTVVHETGGAKGRDERMARR
                     YPDTDVLMFGHSHIPWDSTAATGLRLLNPGSPTDRRRQPQCTYMTATVADGVLSEVAL
                     HRL"
     gene            complement(612..977)
                     /locus_tag="VF874_03665"
     CDS             complement(612..977)
                     /locus_tag="VF874_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016935270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEX9175215.1"
                     /translation="MTFVEVIETVGKVIDATGVAVIVGGALFAAALTVLRRWGQGSRY
                     ESFRRQLGRSILLGLEFLVAADIIRTVAITPTPQSVAVLGGIVLIRTFLSFSLQVEMT
                     GAWPWQRKAATTGDAAPPR"
     gene            1051..2931
                     /locus_tag="VF874_03670"
     CDS             1051..2931
                     /locus_tag="VF874_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX9175216.1"
                     /translation="MRPKPPTETIERAHKEHLGSLPFEDTADFADADRGFIASLQPCV
                     IKAADGRVVWDNDVYSFLAGDAPPSVHPSLWRQSMLAAKQGLYEVVEGIYQVRGLDLS
                     NISFIETDTGVIVIDPLVSTEVAAAALKLYRENRGGDRPVVAVIYTHSHVDHFGGVLG
                     VTNQQDVDDGNVSVLAPEGFVEHAIQENVYAGTAMARRASYMYGTVLERGPRGQVGCG
                     LGQTPSTGEVALINPTVTITTTGEKHTIDGLEIEFQMAPGTEAPAEMHFYFPRYRALC
                     MAENATHNLHNLLTLRGALVRDPHVWSGYLTEAIDHFTDNVDVAFASHHWPTWGRERV
                     IEFLFTQRDLYAYLHDQTLRLLNQGCTGAEIAEQFQMPPVLENAWHTHGYYGSVSHNV
                     KAIYQRYMGWFDGNPARLWPHPPEALAKRYVDALGGIDRVVELAQNAFDEGDFRWAAT
                     LLDHATFADAEHPKVRELYADTLEQLAYGAENATWRNFFLSGATELRDGNFGTATSAA
                     SPSMLSQLTPEQMFDTLAISINGPRAWDLNLAIDVTFSDVDTNYRVTLRNGVLAYRKR
                     SADETTANATVRLDNKMRLLMFAAGDRTSPGIDISGDADALPALLGVLDAPDPKFNIV
                     TP"
BASE COUNT          554 a          976 c          950 g          484 t
ORIGIN      
        1 tcgccgacat cgacggcatc catgtcaacg gcgtcgacat gatccactgg aacgacgacg
       61 agaagatcgt cgagttcaaa gtgatgctgc gaccgctgaa ggcgttgcag gcggtgatcc
      121 cggcgatggc cgagttgctc cagaatccgt gaagctgctg ctgatctccg acacccatat
      181 ccccaagcgc gcccgcgatt tgcccagccg ggtgtgggac gaggtcgaac gagcggacgt
      241 cgtgctgcat gccggcgact gggtggacgt ctcgttgctc gacgcgctag gggagcgggc
      301 gccgcgcctg atcggatgtt ggggcaacaa cgacggccct gctcttcggg ctcgactgcc
      361 cgaagaggcg gacgcgacgc tagaaggcat ccgcttcacc gtggtgcacg aaaccggcgg
      421 cgccaaaggc cgcgacgagc ggatggcgcg gcgctacccc gacaccgatg ttctaatgtt
      481 cggccacagt cacattccgt gggacagcac cgccgcgacg ggtctgcggc tgctgaatcc
      541 aggctcgccc accgaccgac ggcgacagcc gcagtgcacg tacatgacgg cgacggtggc
      601 ggacggcgtg ctcagcgagg tggcgctgca tcgcctgtag tcgcggcttt cctctgccac
      661 ggccaggcgc ccgtcatctc gacttgcagc gaaaagctca ggaacgtccg gatcagcacg
      721 atgccgccga gcactgcgac gctttggggt gtcggggtga tcgcgacggt ccggatgatg
      781 tcggccgcga cgaggaactc caggccgagc aggatcgaac ggccgagctg gcgacggaac
      841 gactcgtacc ggctgccttg cccccaacgc cgcaacaccg tcaaagccgc cgcgaacaac
      901 gcgccgccaa cgatcacggc cacgccggtc gcatcgatca ctttgccgac cgtctcgatg
      961 acctctacga acgtcatcgc tgaatgttaa ccctctgcga atcagccgat ttggatgtgg
     1021 aaggcgtggc gcggtggcac tgtcggatgt atgaggccga agccacccac cgaaacgatc
     1081 gaacgcgcgc acaaggagca cctggggtca ctgcccttcg aggacaccgc cgatttcgcc
     1141 gacgccgacc gcggcttcat cgcgtcgctg cagccgtgcg tgatcaaggc tgccgacggt
     1201 cgcgtggtgt gggacaacga cgtgtactcg ttccttgccg gcgatgcacc gccgtcggtg
     1261 catccgagtt tgtggcggca gtcgatgctc gcggccaagc aggggctgta cgaggtggtc
     1321 gagggtatct accaggtgcg gggactcgac ctctccaaca tcagcttcat cgagaccgac
     1381 accggcgtca tcgtcatcga tccgctggtt tccaccgagg tggccgccgc ggcgctgaag
     1441 ctgtaccggg agaaccgcgg tggcgaccga cccgtcgtcg cggtgatcta cacccacagc
     1501 cacgtcgacc acttcggcgg cgtcctgggc gtcacgaacc agcaagacgt ggacgacggc
     1561 aacgtgagtg tgcttgcgcc ggaaggcttc gtcgagcatg ccatccagga gaacgtctac
     1621 gccggcacag cgatggcgcg gcgcgcgagc tacatgtacg gcaccgtttt agaacgtggt
     1681 ccccgcgggc aggtcggctg cggtctcggg cagacgcctt cgaccggcga agtggcgttg
     1741 atcaacccga cggtaacgat caccaccacg ggcgagaagc acaccatcga cggccttgag
     1801 atcgaattcc agatggcgcc cggcactgag gcccccgccg aaatgcactt ctatttcccc
     1861 cgctaccgcg cactgtgcat ggcggagaac gcaactcaca acttgcacaa cctgctgacg
     1921 ttgcgggggg cgcttgttcg cgacccgcac gtatggtccg ggtatctgac cgaggcgatc
     1981 gaccacttca ccgacaacgt ggatgtcgcg ttcgcctcgc accactggcc gacgtggggc
     2041 cgcgagcgcg tgatcgaatt cctctttacg caacgcgatc tctacgcgta cctccatgac
     2101 caaaccctgc ggctgctgaa ccaggggtgt accggcgccg agatcgccga gcagttccag
     2161 atgccgccgg tcctggagaa cgcgtggcac acccacggct actacggctc ggtcagccac
     2221 aatgtgaagg cgatctacca gcgctacatg gggtggttcg acggcaaccc cgcccggctg
     2281 tggccgcatc cgcccgaagc tttggcgaag aggtacgtcg acgcactcgg cggcatcgac
     2341 cgcgtcgtgg agctcgcgca gaacgcgttc gacgagggtg actttcgctg ggccgcaacg
     2401 cttctcgacc atgcgacatt cgccgacgcc gagcacccga aggtgcgcga gctgtacgcc
     2461 gacacgctgg agcagctcgc ctacggcgcc gagaacgcga cctggcgcaa cttcttcctg
     2521 tccggcgcca ccgagttgcg cgacgggaac ttcggcacgg ctacctcggc cgcgtcaccg
     2581 tcgatgctgt ctcagctcac gccggagcag atgttcgaca cactggcgat aagcatcaac
     2641 ggtccgcggg catgggatct gaacctggct atcgatgtca cgttctccga cgtcgacacc
     2701 aactaccgag tgacgctgcg caacggcgtg ctggcgtacc gcaagcgatc cgctgacgaa
     2761 acgactgcga atgcgactgt gcgtctggac aacaagatgc ggctgttgat gttcgcggcc
     2821 ggcgaccgga catcgcccgg catcgacatc agcggcgacg ccgacgcttt gcctgccttg
     2881 cttggcgtgc tcgacgcacc ggacccgaag ttcaacatcg tcacgccgta gtcatgcgca
     2941 ggactatcga ctgggacggc gacg
//
LOCUS       DASWBF010000105         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_405887,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000105 DASWBF010000000
VERSION     DASWBF010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405887"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2398)
                     /locus_tag="VF874_03675"
     CDS             complement(<1..>2398)
                     /locus_tag="VF874_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX9175217.1"
                     /translation="KALHGFAEDVLGSAHDDVAVLYTNHPKLGDIPSFNQALCGLYAA
                     GLGYAEQAPAKLAAKTETAMTDVAGPAATMTDVAGPAATMTVAQFAELGRHLVDVVQH
                     GVHLQPAVTGSAPPAAEPVVVTGAALGLPGVERVFDDENIARILDGQQFIDTIPHKLR
                     REMLDRHITRLVKRESGDPTFETIDSESEVIKLAGRSAPLDVVSEFGVDTARDAALDT
                     STRLAIGAGFDALRDAGIPLVMHYKATTLGTKLPDRWGLPESMRDDTGVIFASAFPGL
                     DNFAKDLERYYTDRSHREQLSTLEALRARMSDDEPAAAEVDRRIHELRHTIETDPFVF
                     DRRFLFRCLSMGHSQFAEIIGARGPNTQVNAACASTTQAAALAEDWIRAGRCRRVVVV
                     SADDATSDTLLPWLASGFLASGAAATDDVVEEAATPFDQRRHGMILGMGAAALVVESA
                     DSARERGLMPICEMLGAVTANSAFHGTRLDVEHIGGVMEKLLEQAEARGVDRHAIAAN
                     TVFVSHETYTPARGGSASAEIHALRRVFGPDADALVITNTKGFTGHAMGAGIEDVVAI
                     KALETGVVPPVPNYKVPDPELGHLNLSTGGSYPVQYALRLGAGFGSQIAMTLMRWTPV
                     PDGRHRLPDELGYQYRIVDEAAWRRWLAQAAGSDDPQLEVVQHRLRIVDTGPPAKAAP
                     SVQQAPAVQTAPVATETVLVGPGSAPVAATTAAPPPTAPAAAPAPVASAPHGDELTDT
                     VVGIVSELTGYPPDLLDVDLDLEADLGVDTVKQAEIFAAIRGHYGVARDDTLKLRDFP
                     T"
BASE COUNT          320 a          825 c          874 g          379 t
ORIGIN      
        1 gtcgggaagt cgcgcagctt gagggtgtcg tcgcgggcga cgccgtagtg gccgcggatc
       61 gcggcgaaga tctcggcctg tttgacggtg tcgacgccga ggtcggcttc caggtcgagg
      121 tcgacgtcga gcaggtcggg cgggtagccg gtgagctcgg acacgatgcc gaccacggtg
      181 tcggtcagct cgtcgccgtg cggcgccgaa gccaccggag ccggcgcggc tgcgggtgct
      241 gtcggcggtg gcgcagcggt cgtcgcggcg accggcgcgg acccgggccc gaccagcacc
      301 gtctcggtcg ccaccggcgc cgtctgcacc gcaggggcct gctgaaccga cggcgcggcc
      361 ttcgccggcg gaccggtgtc gacgatccgc agccggtgct gcacgacctc cagctgcggg
      421 tcgtcggagc ctgcggcctg cgccaaccac cgccgccacg cggcctcgtc gacgatccgg
      481 tactggtagc caagttcgtc aggcagtcgg tgacgcccgt cgggcaccgg cgtccagcgc
      541 atcaacgtca tcgcgatctg ggacccgaac ccggctccca gccgcagcgc gtactgcacc
      601 ggatagctgc caccggtcga caggttcaga tgacccaact ccgggtcggg caccttgtag
      661 ttcggcaccg gcggaaccac gccggtttcc agcgccttga tcgcgacgac gtcttcgatg
      721 ccggcgccca tcgcgtgtcc ggtgaagccc ttggtgttgg tgatgacgag cgcgtcggcg
      781 tcgggcccga aaacccttcg cagcgcgtgg atttcggccg acgcgctgcc gccgcgggcg
      841 ggcgtgtacg tctcgtgcga gacgaaaacc gtgttggccg cgatcgcgtg ccggtccacg
      901 ccgcgggcct cggcctgctc gagcagtttc tccatgaccc caccgatgtg ctcgacgtcc
      961 agccgggtgc cgtggaatgc gctgttcgcg gtgaccgcgc ccagcatctc gcagatcggc
     1021 atcagcccgc gttcccgggc ggagtcggcg gattccacca cgagcgccgc ggcgcccatg
     1081 ccgaggatca tcccgtgccg gcgctggtcg aacggcgtgg cggcctcctc gacgacgtcg
     1141 tcggtggcgg ccgcgccgga ggccaggaag cccgacgcca gccagggcag cagcgtgtcg
     1201 gaggtggcgt cgtcggcgga caccaccacc acccgtcggc accggccggc gcggatccag
     1261 tcctcggcca gcgccgccgc ctgcgtggtg ctggcgcacg cggcgttgac ctgtgtgttc
     1321 ggcccgcgcg caccgatgat ctcggcgaac tgcgagtggc ccatcgacag gcaacggaac
     1381 aggaagcgcc ggtcgaacac gaacgggtcg gtctcgatgg tgtggcgcag ctcgtggatg
     1441 cgccggtcga cctccgccgc ggccggttcg tcgtcgctca tccgtgcgcg cagcgcttcc
     1501 agcgtgctca gctgttcccg gtggctccgg tcggtgtagt agcgctccag atccttggcg
     1561 aagttgtcca ggccggggaa agcggacgcg aagatcacgc cggtgtcgtc gcgcatcgac
     1621 tccggcaggc cccaccggtc gggcagcttg gtgcccagcg tcgtggcttt gtagtgcatc
     1681 accagcggaa tcccggcgtc gcgcagcgcg tcgaaaccgg ccccgatggc gagccgggtg
     1741 ctggtgtcca gcgcggcgtc gcgggcggtg tcgacgccga actcgctgac cacatccagc
     1801 ggcgcgctgc ggccggccag cttgatgacc tcggactcgc tgtcgatggt ctcgaacgtc
     1861 gggtccccgg attcgcgctt gaccagccgg gtgatgtggc ggtcgagcat ttcgcggcgc
     1921 agcttgtgcg gaattgtgtc gatgaactgc tggccgtcca ggatgcgggc gatgttctcg
     1981 tcatcgaaga cccgctcgac cccgggcagg ccgagtgccg cgccggtcac caccaccggc
     2041 tcggcggcgg gcggtgccga gccggtgacg gctggttgca ggtggacgcc atgttgcacg
     2101 acgtcgacga ggtggcggcc caactcggcg aactgcgcga ctgtcatcgt cgccgccggg
     2161 ccggcgacat ctgtcatcgt cgccgccggg ccggcgacat ctgtcattgc ggtctccgtc
     2221 ttagcggcga gcttggcggg cgcctgctcg gcatagccca gcccggcggc gtacagcccg
     2281 cacagcgcct ggttgaacga ggggatgtcc cccagcttgg ggtggttggt gtagagcacg
     2341 gcgacgtcgt cgtgggccga gccgagcaca tcctcggcaa agccgtgcag ggccttct
//
LOCUS       DASWBF010000106         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_470452,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000106 DASWBF010000000
VERSION     DASWBF010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470452"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1670)
                     /locus_tag="VF874_03680"
     CDS             complement(<1..>1670)
                     /locus_tag="VF874_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX9175218.1"
                     /translation="VDWGLEYLFGVLERDGTFHPLWAHDRAGERAALVTFLAMVGKRR
                     KRYPNMHIYHYAAYEKTALLRLAARHGVGEDEVDDLLRSEVLVNLYPIVRKAILLGTR
                     SYGLKALEPLYMGDELRGGDVTSATDSIVQYARYCALRDGGRGEEAAGVLKEIEDYNH
                     YDCTSTRKLRDWLLLRAFENGVTHLTRPAPPGETVPTDDEIACTLSGFAGDSTTRRSP
                     DQTAAALINAARGYFQRERKPYWWNHFDRLTGPVEEWADTSGVFRVEHAHVETDWHLP
                     PRKRKPRRHVRLSGSLCGGLLDTDVCVLYDRPAPPGLDDTHPDRRAAGRATVIETVDA
                     AAGLPVEVLIEELQPEAGPFHHLPMALTPGFPMRTDAMEQVIETVAAEAAESVRTCAG
                     LPRTAVTDILRRRPPTVAGGAALPRLGDDIASITAALLGLTGSYIAVHGPPGTGKTYS
                     AARVIARLVNEHGWRVGVVAQSHAVVENLLDEIVGAGVDPSVVAKKSGGHYTNPPAWQ
                     VISETRYAVFIADNAGCVIGGTSWDFANAGRIPPGSLDVLAIDEAGQF"
BASE COUNT          250 a          570 c          580 g          270 t
ORIGIN      
        1 gaactgcccg gcttcgtcga tggccagcac gtcgaggctg ccaggcggga tgcgccccgc
       61 gttggcgaag tcccacgacg tgccaccgat gacgcagccc gcgttgtcgg cgatgaagac
      121 ggcgtaccgg gtttccgaaa tcacttgcca tgctggggga ttcgtgtagt gaccgccgga
      181 ttttttcgcg acgaccgacg ggtcgacgcc ggcgcccacg atttcgtcga gcaggttctc
      241 gaccaccgcg tgggactgcg ccaccacgcc gacgcgccag ccgtgctcgt tgaccagccg
      301 ggcgatcacc cgcgcggcac tatacgtctt gccggtaccg ggcggtccgt gcaccgcgat
      361 gtaggagccc gtcaacccca gcagcgccgc cgtgatcgag gcgatgtcgt cacccagtcg
      421 cggcagcgcg gcaccgccgg cgacggtcgg ggggcggcgg cgcaggatgt cggtcaccgc
      481 ggtacgcggc aatcccgcac acgtccgcac cgattcggca gcctcggccg cgaccgtctc
      541 gatgacctgc tccatggcat cggtccgcat cgggaaccca ggagtcagcg ccatcgggag
      601 gtggtggaac ggtcccgcct cgggctgcaa ctcctcgatc agcacctcga ccggcaaacc
      661 ggctgctgcg tcgacggttt cgatcacggt ggccctgccg gccgcgcggc ggtcgggatg
      721 cgtgtcatcg agaccaggcg gcgccggcct gtcgtagagg acgcacacgt cggtgtcgag
      781 cagtccgccg cacaggctgc cgctgagccg gacgtgccgg cgcggcttgc gcttgcgggg
      841 cggcaggtgc caatcggtct cgacgtgcgc gtgctccaca cggaagacgc cggacgtgtc
      901 ggcccattcc tcgacgggcc cggtcaggcg gtcgaagtgg ttccaccagt atggttttcg
      961 ttcgcgctgg aagtatccgc gggccgcgtt gatcagcgcg gcggcggtct ggtcggggga
     1021 gcggcgtgtc gtggaatccc cagcgaaacc gcttagcgtg caagcgatct cgtcgtcggt
     1081 gggcacggtc tcgccgggcg gggcgggacg ggtcagatgt gtgacgccgt tctcgaacgc
     1141 gcgcagcaac agccaatcgc gcagcttgcg agtcgacgtg cagtcgtagt ggttgtagtc
     1201 ctcgatctct ttgagcacgc ccgcggcctc ttcgccgcga ccgccgtcgc gcagcgcaca
     1261 gtaccgggcg tactggacga tcgagtcggt ggcgctggtg acatcgcccc cgcgcagctc
     1321 atcgcccatg tacagcggtt ccagcgcctt gagcccgtag gacctggtgc cgaggagaat
     1381 cgctttgcgc acgatcggat acaggttgac gagcacctcg ctgcgcagca gatcgtcgac
     1441 ctcgtcctcg ccgaccccgt ggcgcgcggc gagccgcagc agcgccgtct tctcatacgc
     1501 ggcgtagtgg tagatgtgca tgttcgggta gcgcttgcgg cgcttgccca ccatcgccag
     1561 gaacgtgacc agcgccgcgc gctcgccggc ccggtcgtgc gcccagagcg gatggaaggt
     1621 gccgtcacgc tcgagcaccc cgaacagata ctccaacccc cagtcgacgc
//
LOCUS       DASWBF010000107         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_338786,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000107 DASWBF010000000
VERSION     DASWBF010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338786"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..393
                     /locus_tag="VF874_03685"
     CDS             157..393
                     /locus_tag="VF874_03685"
                     /inference="COORDINATES: protein motif:HMM:NF036442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5372 family protein"
                     /protein_id="HEX9175219.1"
                     /translation="MGCVVITHPFHPLRGQRLEVLFVKRRGAERVFVCAGGVGDRVTV
                     PRSWTDRGAPALAHRLSIEGLAELAAVTRAIEGC"
     gene            427..777
                     /locus_tag="VF874_03690"
     CDS             427..777
                     /locus_tag="VF874_03690"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HEX9175220.1"
                     /translation="MNIHKAELSTLSTKTLLARRRRLVARLGDVEQVLAGSLVEQTRR
                     CGKAGCGCADGEPHGPYTYFTPKPAGRGRSKYVPASLVAAVRARLVRGEQVAEVLTGI
                     SAINAELLARRELI"
     gene            890..2953
                     /locus_tag="VF874_03695"
     CDS             890..2953
                     /locus_tag="VF874_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9175221.1"
                     /translation="MNAETKITSSHRDRLAVIYLRQSSMAQVREHTESTTRQYGLAEQ
                     AVRLGWDRRDVVVIDTDLGVSGRFGVVREGFAELVSRVCSGRVGAIFGIEISRLARSN
                     AEVARLMEFAAITETLLVDTDGVYDPGDINDRMLLGLKSTMGEVELHVMAQRLQASKK
                     AAAARGELRTPLPVGFVHDEAGQVVLDPDAEVQAAVGDLFAAFAACGSAYGVVAAFAG
                     RRFPLRAYGGAWAGQLRWGRLTHARVLGVLKNPCYAGAYVHGRYTSRRTVDSDGRVHS
                     GVVERSRAEWAVLINDHHGGYIGWDDYLANEAKLAANRTNTGARPPREGSALCQGIIG
                     CGSCGAPMRTNYHANQQASYECSSRANHSTTPTCRSIVAATVDDAVAARLLKALDPTE
                     VALALAAADEITDRCRRVGRAAELAVERARYEAHRAERAFHAVEPENRLVARNLETRW
                     EAKLATLAEAEAAVEAARHQMPPLPTRVDLERLTADLPRLWEAETTSNKDRKRLLRTL
                     IADITVLPEPDHAMARIGLRWHTGATDELRVARVVHPGTAKRSPTPAVEMVMRLGPTT
                     PTDQLADLLNAAGLTTGHGAPFDVKAVQWIRHAYHVPAPTPYAAGEISVADAAERLDC
                     SVGVIYDWIKTGKLAARRGPGNRLCIPWTHQIQAESRYRITESGHLNPARRRTTRRTR
                     TRAEA"
     gene            2953..>3149
                     /locus_tag="VF874_03700"
     CDS             2953..>3149
                     /locus_tag="VF874_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175222.1"
                     /translation="MSVRQAPCPSNIVQHNHIRPEDPNKRLQEGQYETSVPTVADRIA
                     QTVVALQLQPRTESIFHDDSFG"
BASE COUNT          515 a         1041 c         1101 g          492 t
ORIGIN      
        1 gggacaacct ctacaagctg tggaaccgga tgagctccgg cagttacctg ccgggcccgg
       61 tgcgggcggt ggagataccg aaggatcatg gggcggggat cagggtgttg ggtgtgccga
      121 cggttacggc atagtgccgc atggtccagt gaggacttgg gttgtgttgt catcacccat
      181 ccgttccacc cgctgagggg gcagcgcctg gaggtgctgt tcgtcaagcg tcggggtgcg
      241 gagcgggtgt tcgtgtgcgc tggcggtgtc ggcgaccggg tgacggtgcc gcggtcgtgg
      301 accgatcggg gtgcaccggc gctggcgcat cggttgtcga tcgaggggct ggccgagttg
      361 gcggctgtga cacgcgcaat cgagggttgt tgattgtcat ggcatttcac gacacaatct
      421 tgtgtgatga atatacacaa ggcggagctc tcgacgttgt cgacgaagac gttgttggcg
      481 cggcggcgtc gtttggtggc gcgtttgggg gatgtcgagc aggtgttggc ggggtcgctg
      541 gtagagcaga cgcgccgttg cgggaaggcg ggttgcggct gcgccgacgg cgaaccacac
      601 ggcccgtaca cctacttcac gccgaaacct gctggtcggg gccggtcaaa gtatgtgccc
      661 gcgagtttgg tggcggcggt gcgtgcgcgt ctggttcggg gagagcaggt cgccgaggtg
      721 ttgacgggga tctcggcgat caacgccgaa ctgctggcgc ggcgggagtt gatatagccg
      781 atgaccgcta ctgccgccgc gccggcgtcg gttctggtcg ggtccgcgcg tgtggaagtg
      841 gttgtggtgc tggcgaatat ggcgatggcg accctgggag gcggcggcga tgaacgccga
      901 aaccaagatc acgagcagcc accgcgaccg gctcgcggtg atctacctgc ggcagtcgtc
      961 gatggcccag gtgcgcgagc acaccgagtc cacgacacgc cagtacgggc tggccgagca
     1021 ggccgtgcgg ctgggctggg accgccgcga cgtggtggtg atcgacaccg acctgggggt
     1081 ttcgggtcgg ttcggcgtgg tccgggaggg tttcgccgag ctggtgagtc gggtctgctc
     1141 ggggcgggtc ggggcgatct tcgggatcga gatctcccgg ctggcccgct ccaacgccga
     1201 ggtcgcgcgg ctgatggagt tcgccgcgat caccgagacg ctgttggtcg acaccgatgg
     1261 cgtctacgat ccgggcgata tcaacgaccg gatgctgttg gggttgaagt ccacgatggg
     1321 cgaggtggag ctgcatgtga tggcgcagcg gctgcaagcc agcaagaagg ccgccgccgc
     1381 gcgcggggag ctgcggaccc cgctgccggt cggcttcgtc cacgacgagg ccggccaggt
     1441 ggtcctcgac cccgatgccg aggtccaggc cgcggtcggt gacctgttcg ccgcgttcgc
     1501 cgcgtgcggt tcggcctacg gtgtggtcgc cgcgttcgcc ggtcggcggt tcccgctgcg
     1561 cgcctacggc ggcgcctggg ccgggcagct gcgctggggt cgtctcaccc acgcccgtgt
     1621 gctgggagtg ttgaagaatc cctgctacgc cggggcatat gtgcatggcc gctacacctc
     1681 tcggcgcacc gtcgattccg acggccgcgt gcactccggc gttgtcgagc ggtcccgcgc
     1741 cgaatgggcg gtgctgatca acgaccacca cggcggctac atcggctggg acgactacct
     1801 ggccaacgag gccaagctgg ccgccaaccg caccaacacc ggggcccggc cgccacggga
     1861 gggttccgca ctgtgtcagg gcatcatcgg ctgcgggtcc tgcggagccc cgatgcgcac
     1921 caactatcac gccaaccagc aggcgtccta cgagtgttcc agtcgcgcaa accattcgac
     1981 cactccgacc tgccgctcga tcgtcgcggc cacggtggac gacgcggtcg cggccagact
     2041 gctgaaggcg ctcgacccga ccgaggtcgc cctcgcgctg gccgccgccg atgaaatcac
     2101 cgaccggtgt cgccgcgtcg gccgggccgc cgaactcgcc gtcgaacgcg cccgctacga
     2161 ggcccaccgc gccgaacgcg ccttccatgc ggtggagccg gaaaaccgtt tggtggcaag
     2221 gaatctggag acccggtggg aagccaaact cgccaccctg gccgaggccg aggccgctgt
     2281 cgaagctgcc cgccaccaga tgcctccgct accgacacga gtggacctcg agcggttgac
     2341 cgccgacctg cctcggctct gggaagccga aaccaccagc aacaaggacc gaaagcgcct
     2401 gttgcgcacg ctgatcgccg acatcaccgt gctgccggag cccgatcacg ccatggcgcg
     2461 gatcgggctc cgctggcaca ccggcgcaac cgacgaactg cgggtggcgc gcgtcgttca
     2521 ccccggaacc gccaaacgca gccccacccc agcggtcgag atggtcatgc ggctcggacc
     2581 gaccaccccc accgaccagc tcgctgacct actcaacgcc gccggcctga ccaccggcca
     2641 cggagcaccg ttcgacgtca aggccgtcca atggatccgc cacgcctacc acgtccccgc
     2701 cccgacgccc tacgccgcag gtgagatcag cgtcgccgac gccgccgaac gcctcgactg
     2761 cagcgtcggc gtgatctacg actggatcaa gaccggaaaa ctcgccgcac gccgcggccc
     2821 cggcaaccgg ctctgcatcc cctggaccca ccagatccag gccgaaagcc gttaccgcat
     2881 aaccgagtcc ggccacctca acccagcacg ccgccgcacc acacgccgca cccgcacccg
     2941 cgcagaggcg taatgtcggt gcggcaggca ccatgtccat caaacatcgt tcagcacaac
     3001 cacatccgac ccgaagatcc caacaagcga ttgcaggaag gacagtatga aacatccgtc
     3061 ccgacggtgg cggaccggat cgcgcagacg gtggtggcgc tgcagttgca gccccgcacg
     3121 gagtcgatct ttcatgacga ctcctttgg
//
LOCUS       DASWBF010000108         8105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1125441,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000108 DASWBF010000000
VERSION     DASWBF010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125441"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1681)
                     /gene="groL"
                     /locus_tag="VF874_03705"
     CDS             complement(59..1681)
                     /gene="groL"
                     /locus_tag="VF874_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814389.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9175223.1"
                     /translation="MSKQIEYSETARRAMEAGVDKLADTVRVTLGPRGRHVVLAKAFG
                     GPTVTNDGVTIAREIELEDPFENLGAQLVKSVATKTNDVAGDGTTTATVLAQAIVKGG
                     LRNVAAGANPIALGIGISKAADAVSDALLAAAIPVTEKQSIAQVATVSSRDEEIGEMV
                     AEAMTQVGHDGVVTVEESSTMNTELEVTDGVQFDKGFLSAYFITDFDAMEAVLEDAYV
                     LLHRDKISSLPDFLPLLEKVAQSGKPLLIIAEDVEGEPLSTLVVNAIRKTLKTVAVKA
                     PFFGDRRKAFLEDLAVVTGAQVVNPDVGLLLREVGLEVLGTARRIVATKDATTIVDGG
                     GGEEAVAARAKQLRAEIETTDSDWDREKLEERLAKLSGGVAVIKVGAATETALKERKE
                     SVEDAVAAAKAAVEEGIVAGGGSALVHARGALDELRASLSGDAALGVDVFAGALSAPL
                     YWIAANAGLDGAVAVNKVAELPKGQGLNAATLSYGDLVADGIVDPVKVTRSAVLNAAS
                     VARMILTTETAIVEKPAEEEDDGHGHGHHHHH"
     gene            complement(1768..2070)
                     /gene="groES"
                     /locus_tag="VF874_03710"
     CDS             complement(1768..2070)
                     /gene="groES"
                     /locus_tag="VF874_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9175224.1"
                     /translation="MAKVNIKPLEDKILVQANEAETTTASGLVIPDTAKEKPQEGTVV
                     AVGPGRWDEDGEKRIPLDVSEGDTVIYSKYGGTEIKYNGEEYLILSARDVLAVVNK"
     gene            complement(2282..3304)
                     /gene="tsaD"
                     /locus_tag="VF874_03715"
     CDS             complement(2282..3304)
                     /gene="tsaD"
                     /locus_tag="VF874_03715"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX9175225.1"
                     /translation="MIVLAIESSCDETGVGIAELGPQGEVRLLVDEVASSVDEHARFG
                     GVVPEIASRAHLEALGPTMRRALAAAGIARPDAVAATIGPGLAGALLVGVAAAKAYAA
                     AWGVPFYAVNHLGGHLAADVYDHGPLPECVGLLVSGGHTNLLHLRSLGEPVIELGSTV
                     DDAAGEAYDKVARLLGLGYPGGKVLDELARTGDRDAVVFPRGMTGPRDDPYSFSFSGL
                     KTAVARYLHRQPDYVAADVAAGFQEAVADVLTAKAVRAANQLGVTTLLIAGGVAANSR
                     LRELAGERCAAAGLTLRVPRPRLCTDNGAMIASLAAHLIAAGARPSALAAAADPGLPV
                     VRSQVF"
     gene            complement(3301..3753)
                     /gene="rimI"
                     /locus_tag="VF874_03720"
     CDS             complement(3301..3753)
                     /gene="rimI"
                     /locus_tag="VF874_03720"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX9175226.1"
                     /translation="MTVTIDALTAADAARCAELEAQLFEGDDPWPAKAFLSELDGRHN
                     HYVAARICDKLVGYAGIARLGILPPHEYEIHTIGVDPAFHGQGIGRRLLDELLAFAGN
                     GTVFLEVRTDNAPAIALYRSAGFVEVGLRKRYYRVSDADAYTMRRESQ"
     gene            complement(3750..4400)
                     /gene="tsaB"
                     /locus_tag="VF874_03725"
     CDS             complement(3750..4400)
                     /gene="tsaB"
                     /locus_tag="VF874_03725"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX9175227.1"
                     /translation="MGVEQMILLALDTATPAVTAGVVRRDEGEHLIAERVTVDARAHA
                     ETLTPNVLAAVADSGLQMTDLDAVVIGCGPGPFTGLRVGMATAAAFGHALDIPVYGVC
                     SLDAIGMGTSGDVLVVTDARRREVYWARYRDGIRTAGPAVDAPADVPCQGAAAVAGSP
                     DHAEMFPLPRLDAEYPTPVGLVRAVADWTGGPKPLVPLYLRRPDAKTLAERQAVTR"
     gene            complement(4382..4918)
                     /locus_tag="VF874_03730"
     CDS             complement(4382..4918)
                     /locus_tag="VF874_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX9175228.1"
                     /translation="MASTATLPTAEDTFTLGARLGADLRAGDVVVLSGPLGAGKTLLA
                     KGIAHAMDVDGAVISPTFVLARVHPARRVGAPAMVHVDVYRLLEHTADARSKTGSADA
                     RSKTVVSADARSKTAVSADLLAELDSLDLDTDLADAVVVVEWGEGLAERLSEHHLDVR
                     LRYDDDSETRTATWEWSR"
     gene            complement(4911..5978)
                     /locus_tag="VF874_03735"
     CDS             complement(4911..5978)
                     /locus_tag="VF874_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175229.1"
                     /translation="MAGVAGLAAVGTVAGVSAARSARHRDAVEDPHATEDFTLLDVDR
                     GSVVTTDDGVPLAAREVGPADAQLTVVFVHGFCMCMGAYHFQRARLEEEWGHQVRMVF
                     YDQRGHGQSGRAAIQTYTVEQLGRDLEAVLAVTVPRGPVVLVGHSMGGMAVLSHARQF
                     PQNYGSRVVGAALISSAAEGVARSPLGEILKNPALEAVRFAARYAPKLVMRARGSVRS
                     LIRPILRTASYGDEKISPAVVAYSEKMIHDTPVETMVEFLHALEVHDESAALPVLETI
                     PTLVACGDRDLLTPAGDSREMAAALPKCELVIVPGAGHLVQLQEPEVIDDALVRLVER
                     ATPSKLVALARRVRERVRRSG"
     gene            complement(6121..7290)
                     /gene="alr"
                     /locus_tag="VF874_03740"
     CDS             complement(6121..7290)
                     /gene="alr"
                     /locus_tag="VF874_03740"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX9175230.1"
                     /translation="MHTLPLTTPLTPNTTPQAVVDLDAIANNIGVLREHAGSAEVMAV
                     VKADGYGHGATRVSRAVLAAGAAELGVATIDEALALRADGITAPILAWLHAPGTDFAP
                     ALSDDVQIAVSSVRQLSELLDAVRRTGITAAVTVKIDTGLNRNGVSDAEYPAMLDALL
                     RAQAEEAVRVRGLMSHMAHADQPEHPTNDLQAQRFSDMLGQARSAGVRYEVAHLSNSP
                     AAMSRPDLAFDMVRPGIAVYGLSPIPERGQMGLIPAMTLRCPVALVKSVHAGDGVSYG
                     HTWVADRDTTLALLPIGYADGVYRALSGRFEVMINGRRRPSVGRVCMDQFVVDLGPGE
                     TGVREGDTAILFGPGTDGEPTAQEWADLLGTIHYEVVTSLRGRVTRVYREGGTIE"
     gene            complement(7343..7738)
                     /locus_tag="VF874_03745"
     CDS             complement(7343..7738)
                     /locus_tag="VF874_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HEX9175231.1"
                     /translation="MTTPGRERVLLDTSVLIGPDADFSGLVCQISSVSYAELHYGVQA
                     ARDPDTRDARQRRLTLIQATFGHGIAFTDVIAHHYGMLCARLRAQGRSPRGRALGLMI
                     AATAAALEVPLVTRNPAEVGDIGIALMQR"
     gene            complement(7719..7967)
                     /locus_tag="VF874_03750"
     CDS             complement(7719..7967)
                     /locus_tag="VF874_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9175232.1"
                     /translation="MEKVGVRQLRQNPTPVLRAVEAGAEVAVTVNGRFVARMVPFDAV
                     DWVDGPRVGQVFAAHVDDEWAAELETSRRAETVDDPWS"
BASE COUNT         1215 a         2956 c         2633 g         1301 t
ORIGIN      
        1 aaacaccgcc ccaccaggcg aaaccagccc aacaatcagc aaaggccgga aactcccgct
       61 agtggtgatg atggtggccg tgtccgtggc cgtcgtcctc ctcctcggcc ggcttctcca
      121 caatcgcggt ctcggtggtg aggatcatcc gagccaccga tgcggcgttg agcaccgcgg
      181 atcgggtgac cttcaccggg tcgacgatgc cgtcggcgac caggtcgccg taggacagcg
      241 tcgccgcgtt cagcccctgc cccttcggca gttcagcgac cttgttcacc gccaccgcac
      301 cgtcgaggcc cgcgttggcc gcgatccagt acagcggggc ggacagcgca ccggcgaaca
      361 cgtcgacacc cagcgcggca tcgcccgaca ggctggcccg cagctcgtcg agcgcgccgc
      421 gcgcgtgcac cagcgcagac ccgccgcccg cgacgatgcc ctcttcgacg gccgccttcg
      481 ccgccgcgac cgcgtcctcg acgctctcct tgcgctcctt cagcgcggtc tccgtcgccg
      541 cgccgacctt gatcaccgcc acacccccgg acagcttcgc cagccgctcc tcgagcttct
      601 cgcgatccca gtcggagtcg gtggtctcga tctcggcgcg caactgcttc gcccgcgccg
      661 cgacggcctc ctcgccgccg ccgccgtcga cgatcgtcgt ggcgtccttg gtggcgacga
      721 tgcgccgggc cgtgcccagc acctcgagtc cgacctcgcg cagcagcagg ccgacgtcgg
      781 ggttgaccac ctgggcgccc gtcacgacag cgaggtcctc gaggaacgcc ttgcggcggt
      841 caccgaagaa cggcgccttg accgcgaccg tcttcagcgt cttgcggatg gcgttgacca
      901 ccagcgtgga cagcggctca ccctcgacgt cctcggcgat gatcagcagc ggcttgccgg
      961 actgcgcgac cttctccagc aacggcagga agtcgggcaa cgagctgatc ttgtcgcggt
     1021 gcagcagcac ataggcgtct tcgagaaccg cttccatcgc gtcgaagtcg gtgatgaagt
     1081 acgccgacag gaatcccttg tcgaactgca caccgtcggt gacttccagc tcggtgttca
     1141 tcgtcgacga ctcctcgacg gtgacgacgc cgtcgtggcc gacctgggtc atcgcctcgg
     1201 cgaccatctc gccgatctcc tcgtcgcgcg acgacacggt ggccacctgc gcgatcgact
     1261 gcttctcggt gaccgggatg gccgcggcca gcagcgcgtc ggacaccgcg tcggccgcct
     1321 tggaaatgcc gatgccgagc gcgatcggat tcgcgccggc agcgacgttg cgtaggcctc
     1381 ccttgacgat cgcctgggcc agcacggtgg cggtggtggt gccgtcgccg gccacgtcgt
     1441 tggtcttggt cgccaccgac ttgactagct gcgcgccgag attctcgaac ggatcctcca
     1501 gctcgatctc gcgggcgatc gtcaccccgt cattggtgac cgtcgggccg ccgaacgcct
     1561 tggccagcac cacatgccga ccgcgcggtc cgagcgtgac ccgtacggtg tcggcgagct
     1621 tgtcgacgcc ggcttccatc gcccggcgcg cggtctcgct gtattcaatc tgcttgctca
     1681 tctgagtcct tgcttaaggg gtttaacgtg cgccgccccg gaaatccgcc gcgcgtacgc
     1741 ggtggatccc ggggcggaca cgggtgatta cttgttgacg acagccagca cgtcgcgcgc
     1801 cgacaggatc aggtactcct cgccgttgta cttgatctcg gtgccgccgt acttgctgta
     1861 gatcacggtg tcaccctcgg agacgtccag cggaatccgc ttctcgccgt cctcatccca
     1921 gcggccggga ccgacggcga caacggtgcc ttcctgcggc ttctccttgg cggtgtcagg
     1981 aatgaccaga ccggatgcgg tcgtggtctc ggcctcattg gcctggacga ggatcttgtc
     2041 ctcgagtggc ttgatgttca ccttcgccac gatgtgagcc ctccacttat ctgggtgcgg
     2101 cccgggcctc tcccgggccg gtgtcttctg tcctggcccg ggcagcgccc agacctgttc
     2161 ggcatttcag gtttcggcat gcgccaccgc cgctgagccg tcgtcgcggg tgccgacccg
     2221 agaggcagcg cgcctgccac ctagcactct atacacgcga gtgctagcac tcaagcacgg
     2281 gctagaacac ctgggatcgg acgaccggaa gccccgggtc ggcagcggcc gccagcgccg
     2341 acgggcgcgc gccggcggcg atcaagtgcg cggcgagcga cgcgatcatc gcgccgttgt
     2401 cggtgcacag ccgcggccgc ggcacccgca gcgtgagtcc ggcagcggcg cagcgctcgc
     2461 cggcgagctc acgcagccgc gagttcgccg ccaccccgcc ggcgatcaac agcgtggtga
     2521 cgcccagctg gttcgccgcc cgcaccgcct tcgcggtcaa cacgtcagcg accgcctcct
     2581 ggaagcccgc cgccacgtca gcggccacgt agtccggttg acggtgcagg taacgggcga
     2641 ccgcggtctt cagcccggag aagctgaacg agtacgggtc gtcgcgcggg ccggtcatcc
     2701 cgcgcggaaa caccaccgca tcgcggtcgc cggtgcgggc cagctcgtcg agcaccttgc
     2761 cgcccgggta gcccagcccc aacaaccgag cgaccttgtc gtaggcctcg cccgccgcgt
     2821 cgtccacggt gctgcccagc tcgatcaccg gctcgcccag cgaccgcagg tgcagcaggt
     2881 tggtgtgccc gccggacacc agcagcccga cgcactccgg cagcggcccg tggtcgtaca
     2941 cgtcggcggc caagtgcccg ccgaggtggt tgaccgcata gaacggcacg ccccacgccg
     3001 ccgcgtacgc cttggcggcg gccaccccga ccagcagcgc gccggccaga cccggcccga
     3061 tcgtcgcggc gaccgcatcc ggccgcgcga tccccgcggc ggccagcgcg cggcgcatcg
     3121 tcgggccgag cgcctccaag tgggcgcgcg acgcgatctc cgggaccacg ccgccgaacc
     3181 gcgcgtgttc gtcgacgctg gaggcgacct cgtcgaccag cagcctgacc tcgccttgtg
     3241 ggccgagttc ggcgatcccg acgccggtct cgtcgcagga gctctcgatc gcgaggacga
     3301 tcattggctc tcccgccgca tcgtgtaggc gtcggcgtcg ctcacccggt agtacctctt
     3361 gcgcagcccg acctcgacga acccggcgct gcggtacagc gcgatcgcgg gtgcgttgtc
     3421 ggtgcgcacc tccaggaaga ccgttccgtt gcccgcgaag gccagcagct catcgagcag
     3481 ccgccggccg attccctggc catgaaaggc ggggtcgacg ccgatggtgt ggatctcgta
     3541 ttcgtgcggc ggcaggatgc ccagccgggc gatgccggcg tagccgacga gcttgtcgca
     3601 gatgcgggcg gccacgtagt gattgtgccg gccgtcgagc tcggacagga aagcctttgc
     3661 gggccacggg tcgtcgccct cgaacagctg ggcttccaac tctgcgcagc gtgccgcgtc
     3721 ggcggccgtc agggcgtcga tcgtgaccgt catcgcgtaa ccgcctgccg ttcggcgagg
     3781 gtcttcgcat cagggcgccg aaggtacagc ggtacaagcg gtttcgggcc ccccgtccag
     3841 tcggcgacag cgcgtaccaa cccgaccggc gtggggtact cagcgtccag acgcggcaac
     3901 gggaacatct ccgcgtggtc cggcgacccg gcgacggcgg cggcaccctg gcacgggacg
     3961 tccgcgggcg catcgaccgc agggccggct gtgcggatcc cgtcgcggta gcgcgcccaa
     4021 tacacctcgc gccgacgggc gtcggtgacc acgaggacgt ctccgctggt gcccatgccg
     4081 atggcgtcga gactgcagac gccgtacacc gggatgtcga gggcgtgccc gaatgccgcg
     4141 gcggtggcca tgccgactcg cagaccggtg aacggccccg gcccgcagcc aatcacgacg
     4201 gcatccagat ctgtcatctg taggccagaa tctgccacgg cggcaagcac attgggcgtc
     4261 agtgtctctg cgtgcgcgcg tgcgtcgacg gtcacccgct cggcgatcaa atgctcgccc
     4321 tcgtcccggc gcaccacccc agcggtcacc gctggcgtcg cggtgtccaa tgccagcagg
     4381 atcatctgct ccactcccat gtggcggtgc gggtttcgga gtcgtcgtcg tagcgcagcc
     4441 ggacgtcgag gtggtgttcg gagagccgtt cggccagccc ctctccccat tcgacgacga
     4501 cgacggcgtc ggccagatcg gtgtcgaggt cgagcgagtc gagttcggcg agcaggtcgg
     4561 cgctgacggc tgtcttgctc ctcgcgtccg cgctgacgac tgtcttgctc ctcgcgtccg
     4621 cgctgcctgt cttgctcctc gcgtccgcgg tgtgctccag cagccggtac acgtcgacat
     4681 gcaccatcgc cggcgctccg actcgccggg cgggatggac gcgcgccaac acgaacgtcg
     4741 gggagatcac cgcgccgtcg acgtccatgg catgcgcaat tcccttggcc agcaaggtct
     4801 ttccggcacc aagcggtccg gagagcacca ccacgtcgcc ggcgcgcaag tccgcgccca
     4861 gccgggcgcc caacgtgaac gtgtcctcgg cggtgggcag cgtcgcggta ctagccactg
     4921 cggcggaccc tttcccgcac ccgccgggcc aacgcgacca gcttggacgg cgtcgcccgc
     4981 tccaccagtc gcacgagcgc gtcgtcgatc acctccggct cctgaagctg cacgaggtga
     5041 ccggcacccg gaacgatcac cagctcgcac ttcggaagcg ctgccgccat ttcccgcgaa
     5101 tcgcccgccg gtgtcaggag atcccggtcc ccgcacgcga ccagcgtcgg gatcgtctcg
     5161 agcaccggca acgcggcgct ctcgtcgtgc acctccagtg catggagaaa ctcgaccatc
     5221 gtctcgaccg gagtgtcgtg aatcatcttc tccgagtacg ccaccaccgc tgggctgatc
     5281 ttctcgtcgc cgtaggacgc ggtgcggaga atcggcctga tcagcgaccg caccgagccg
     5341 cgcgcccgca taacgagttt gggcgcgtag cgggcggcga atcgcacggc ctccagcgcg
     5401 gggtttttca ggatctcccc aagtggggat cgtgccactc cttcggccgc tgacgagatc
     5461 agcgcggcgc cgacgacacg cgagccgtag ttctgcggga actgccgcgc gtgcgacagc
     5521 accgccatgc cgcccatcga gtgcccgacg agcacgaccg gcccgcgcgg aaccgtcacc
     5581 gccagcacgg cttccagatc gcggccgagc tgctccacgg tgtacgtctg gatcgcggca
     5641 cgcccggact gaccgtggcc acgctggtcg tagaacacca tccggacctg gtggccccac
     5701 tcttcttcga ggcgggcccg ctggaagtgg taggcgccca tgcacatgca gaaaccgtgc
     5761 acgaatacca cggtcagctg ggcgtcggcc gggcccactt cccgagccgc caacggcacg
     5821 ccgtcgtcgg tggtgacgac gctgccgcgg tcgacgtcga gcagcgtgaa atcctctgtc
     5881 gcatgcgggt cctcgaccgc atcccgatgc cgcgccgacc gggcagccga gacgccggcg
     5941 acggttccga ccgcagccag cccggcgacg cccgccatcc agcgtgcgtc cttgaagcgc
     6001 tcgcgctccg gctgctcgcc acttgccagt ttggccagcc gctcggcgtt cttcgccgcc
     6061 gtgcgggcaa ggctgtcgtc gatgcgctgc gcgcgcttgg cgcgtcgctc tgatttcttg
     6121 tcactcaatg gttccgccct cgcggtagac acgggtgacc cggccgcgca gactggtcac
     6181 cacctcgtag tggatcgtgc cgagcaggtc ggcccactcc tgggcggtcg gctcaccgtc
     6241 tgtgcccggc ccgaagagga tcgccgtgtc accttcgcgc acaccggttt cgcccggacc
     6301 gaggtcgacg acgaactggt ccatgcacac ccggccgacg ctgggtcgcc tgcggccgtt
     6361 gatcatcact tcgaaacggc cgctcaacgc ccggtacacg ccgtcggcgt atccgatcgg
     6421 gagcagcgcc aacgtcgtgt cccggtcggc gacccaggtg tgcccgtacg acacgccgtc
     6481 acccgcatgc accgacttca cgagcgccac ggggcatctc agcgtcatcg ccgggatcag
     6541 gcccatctgc ccgcgttccg gaattgggct gagtccgtac accgcgatgc cgggcctgac
     6601 catgtcgaag gccaggtcgg gccgcgacat cgccgccggt gagttggaca ggtgcgcaac
     6661 ctcgtagcgc acgcctgccg atcgcgcctg gcccagcatg tcggaaaacc gttgtgcctg
     6721 aaggtcattg gtcggatgct cgggctggtc ggcgtgcgcc atgtgcgaca tcagcccacg
     6781 cactcgcacc gcttcctcgg cctgggcgcg cagcagcgcg tcgagcatcg ccgggtactc
     6841 ggcgtcgctc accccgttgc ggttcagccc ggtgtcgatc ttgaccgtga cggcggcagt
     6901 tattcccgtt cgccgcaccg cgtcgagcag ctcggacagt tggcgcaccg acgacaccgc
     6961 gatctgcacg tcgtcagaaa gcgccggcgc gaagtcggtg cccggcgcgt gcagccacgc
     7021 caggatcggc gcggtgatgc cgtcagcgcg cagcgcgagc gcctcgtcga tggtggccac
     7081 tccgagctcg gcggcaccgg cggccagcac ggcccgcgat acccgggtcg cgccgtgccc
     7141 gtaaccgtcg gccttgacga ccgccatcac ctcggccgat cccgcatgct cccgcagtac
     7201 gccgatgttg ttcgcgatcg cgtcgagatc gaccacggcc tgcggcgtgg tgttcggggt
     7261 caacggcgtg gtcagcggaa gtgtgtgcat gctcagccga ttgtcccaga acgcgcccgt
     7321 gcggcttccc cgtcacctct cattaccgct gcatcagcgc aatcccgatg tcgccgacct
     7381 ctgctggatt gcgcgtcacc aacggcactt cgagcgctgc ggcggtcgcg gcgatcatca
     7441 aaccgagggc ccgaccacgg ggactgcggc cctgtgctcg cagccgcgcg caaagcatgc
     7501 cgtagtgatg agcaatgaca tcggtgaaag cgatcccgtg tccgaatgtc gcctgtatga
     7561 gggtcaaacg acgctgtcgc gcgtcgcgcg tgtccggatc acgggcggcc tgcactccgt
     7621 agtgcagctc ggcgtaacta accgagctga tttgacacac cagtcccgag aagtctgcgt
     7681 cgggcccaat caagacgctg gtatccagca gcacccgttc acgaccaggg gtcgtcaacc
     7741 gtctcagccc gtcgtgacgt ctcgagttcg gcagcccatt cgtcgtcgac atgcgccgcg
     7801 aagacctgtc cgacgcgcgg accgtcgacc caatcgacag cgtcgaacgg caccatccgg
     7861 gcgacgaaac ggccattcac tgtcacggcg acctcagcgc cggcctccac cgcccgcagt
     7921 accggcgtcg ggttctgacg cagttgccgc acgccgacct tctccatgcg tccaggctac
     7981 ccggtgtaga ccatgatcta catcaggttt ttcgcgagcg ccagctccaa ctcagtgcgc
     8041 gaagtgctgt tgctggtcga aatggccgcc cggtttgagc tcgtccatgt gggcgaccac
     8101 cttcg
//
LOCUS       DASWBF010000109         4109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_534775,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000109 DASWBF010000000
VERSION     DASWBF010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534775"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VF874_03755"
     CDS             <1..332
                     /locus_tag="VF874_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEX9175233.1"
                     /translation="SVVQQLIDVGAVEPSEAESLPMRNQLTRGLFAQRDEPADMWLLR
                     MTPADRIMACSDGLPKEVDSSTIERMLREIPDPQAVAGELVNAALDAGGRDDVTVVVV
                     DAVAVAS"
     gene            complement(329..979)
                     /locus_tag="VF874_03760"
     CDS             complement(329..979)
                     /locus_tag="VF874_03760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175234.1"
                     /translation="MTEAQRLFALADEVTGFMPADEGRALYEAALKYLADGAAVEIGT
                     YCGKSTLLLGAAAQERNSVLYTIDHHHGSEEHQPGWEFHDVSMIDTVTGRFDTLPTLR
                     RTLDAADLDDNVVAVVGKSTTVARGWRTPLQFVFIDGGHSEAAAAADFDGWAKWVTVG
                     GALVIHDVFPNPDDGGQAPYHIYWRAVGSGQFREAAATGSLRVLERTAGQPGEEVR"
     gene            1044..1619
                     /locus_tag="VF874_03765"
     CDS             1044..1619
                     /locus_tag="VF874_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9175235.1"
                     /translation="MSESVSLESTRRRLTAKQADTIQRLSNAALAMLAREGFGGLTIR
                     LVAAEAGVGAATAYTYFSSKEHLVAEVFWRRLASSVPPPIDDQPPVERVIAVLRHIAL
                     LVADEPEFAGAVTNALLGRDPDVEHLRDRIAREIRGRLVEAAGEGTDPALVETLELLY
                     TGALVHAGMGYVTYSEIADRLERSARLLLSA"
     gene            complement(1680..2102)
                     /locus_tag="VF874_03770"
     CDS             complement(1680..2102)
                     /locus_tag="VF874_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase family protein"
                     /protein_id="HEX9175236.1"
                     /translation="MSDKVIEVSPALAASQASWKCVQAGNREGWLALMADDVLIEDPI
                     GEAVTNPDGLGVRGKEAVGAFFENTIARSEATITCEETFPSSSPKEIAHILLLHNKFD
                     GFSTTVRGVFTYRVNDAGLLTHLRGYWSMDMMQIRKEP"
     gene            complement(2118..3245)
                     /locus_tag="VF874_03775"
     CDS             complement(2118..3245)
                     /locus_tag="VF874_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HEX9175237.1"
                     /translation="MKTKGALLWEFNQPWSIEEIEIGDPVKDEVKIQMEASGMCHSDH
                     HLVTGGIPMGGFPVLGGHEGAGIVTEVGPGVEDIAPGDHVVLSFIPSCGRCPSCQAGM
                     RNLCDLGAGLLNGAAVSDGTFRIQARGQNVFPMTLLGTFSPYMVVHKSSVVKIDPSIP
                     FEVACLVGCGVTTGYGSATRSADIRPGDDVAIVGIGGVGMAALQGAVNAGARRIFAID
                     PVEWKRDQALKFGATHVYPDIDAAMIGMAEVTYGLMAQKVVVTVGELKGEDIDSYINI
                     TAKGGTCVLTAIGSLLDTNVTLNLAMLTLMQKNLQGTIFGGGNPHHDIPLLLSMYKAG
                     KLNLDDMITRQYRLEQINDGYQDMLEGRNIRGVIRYTDADR"
     gene            complement(3360..3905)
                     /locus_tag="VF874_03780"
     CDS             complement(3360..3905)
                     /locus_tag="VF874_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9175238.1"
                     /translation="MTSFPREELEDWVERWLEANRKAERKGDWTPLADFYDDEATYGW
                     NIGPKEDVMCVGIDEIRDIALGLEMAGLENWHYPYQRVIIDDKIGEIVGFWKQVATDA
                     DAVESEIYGIGGSWFRLAANGKIEWQRDFFDFGHVQKLYLDLMKAGKLSEGMQKRIER
                     SLAGEKLPGYYPLGKAPAPLW"
BASE COUNT          660 a         1332 c         1323 g          794 t
ORIGIN      
        1 attcggtcgt ccagcagctg atcgatgtcg gagcggtcga gccatccgaa gcagagtctc
       61 ttccgatgcg caatcagctc acgcgtggat tgtttgcaca gcgcgacgaa ccggccgaca
      121 tgtggttgct tcgaatgaca cccgctgatc ggatcatggc ctgctcggac gggctgccca
      181 aagaagtcga cagctcgacg atcgagagaa tgttgcgcga gatacccgac ccgcaagccg
      241 tggcgggcga attggtcaat gccgccttgg atgcgggagg ccgcgacgat gtcaccgtgg
      301 tcgtggtcga cgctgtcgcg gtcgccagtt agcggacctc ttcgcccggc tggcccgctg
      361 tccgctccag cacgcgcaac gaaccggtgg ccgcggcttc ccgaaactgg ccggagccaa
      421 ctgcccgcca gtagatgtgg tacggcgcct ggccgccgtc gtcggggttg gggaacacgt
      481 cgtggatgac cagggcgccg ccgacggtca cccacttcgc ccagccgtcg aagtcggcgg
      541 cggccgccgc ctcgctgtgc ccgccgtcga tgaacacgaa ctgcagcggc gtgcgccagc
      601 cgcgcgccac cgtcgtcgac ttccccacga ccgcgacgac attgtcgtcg agatcggccg
      661 cgtcgagcgt gcgtcgtagc gtcggcagcg tgtcgaaccg cccggtcact gtgtcgatca
      721 tcgacacgtc gtgaaattcc cagccgggct ggtgctcctc ggagccgtgg tggtgatcga
      781 tggtgtacag cacgctgttc cgttcttggg cagccgcgcc gagcagcagg gtcgacttgc
      841 cgcagtaggt gccgatctcg actgcggccc cgtcggccag gtacttcagc gcggcctcat
      901 acagcgcgcg gccctcgtcg gccggcatga acccggtgac ctcgtcggca agcgcgaaca
      961 gccgttgggc ttcggtcatg gacctcaacc taatcggtga ggccgattgc cggccatccg
     1021 ggtgtgtagt agtgtctgga cacgtgtccg aatcggtgtc tctggagtcc acccgacgcc
     1081 ggctcacggc caagcaggcc gacaccatcc agcggctgag caacgctgcg ctcgccatgc
     1141 tcgcccgaga aggcttcggc ggcttgacga ttcgattggt ggccgccgag gcgggcgtcg
     1201 gagccgccac cgcctatacg tacttctcgt cgaaggagca tctggtcgcg gaggtgttct
     1261 ggcgtcgcct cgcgtcgtcg gtgccgccac ctatcgacga ccagccaccg gtcgagcggg
     1321 taatcgcggt gctgcgtcac atcgccctgc tcgttgcgga cgaacctgag ttcgccggcg
     1381 cggtcaccaa tgctctactg ggccgtgatc ccgacgtcga gcacctgcgg gaccgcatcg
     1441 cgcgggagat caggggccgc ctggtcgagg cggccgggga gggtacggat ccggcgttgg
     1501 tcgagaccct cgagttgctt tacaccggcg cgctggtgca tgcgggaatg gggtacgtga
     1561 cgtattcgga gatcgccgat cgcctggaga ggtcagcccg tctgctgctt tccgcgtgag
     1621 tgtgcggatt tgtacgcttc caccggcgcg tcgcgtacga aaccgcacgc tcggcgcagt
     1681 cagggctcct tgcggatctg catcatgtcc atgctccagt aaccgcgcag gtgggtgagc
     1741 agtccggcat cgttgacgcg ataagtgaag acaccgcgta ctgtggtgct gaatccgtcg
     1801 aacttgttgt gtaacagcaa gatgtgcgcg atctccttcg gtgagctcga cgggaacgtc
     1861 tcctcgcaag tgatggtcgc ctcactgcgc gctatcgtgt tctcgaagaa cgcgccgacg
     1921 gcctccttgc cgcgcacacc gagtccgtcg gggttcgtga cggcctcgcc gatggggtcc
     1981 tcgatgagca cgtcgtcggc catcagcgcc agccagcctt cgcggttgcc ggcctgcacg
     2041 cacttccacg acgcctgcga cgccgcgagc gccggcgata cctcaattac tttgtcagac
     2101 atgggaatac ggtcgggcta acggtcggcg tccgtgtaac ggatcacgcc gcgaatgttg
     2161 cggccttcca acatgtcttg gtagccgtcg ttgatctgct ccagcctgta ctggcgggtg
     2221 atcatatcgt cgaggttcag cttgcccgcc ttgtacatcg acagcagcag cgggatgtcg
     2281 tggtgcgggt tgccgccgcc gaagatggtg ccctgcaggt tcttctgcat cagggtcagc
     2341 atcgcgaggt tcagggtgac gttggtgtcg agcaggctgc cgatggccgt cagcacgcag
     2401 gttccgccct tggcggtgat gttgatgtag ctgtcgatgt cttcgccctt gagctcgccg
     2461 acggtgacga ccactttctg ggccatcagc ccgtacgtca cctcggccat gccgatcatc
     2521 gcagcgtcga tgtccgggta gacatgcgtg gcgccgaatt tgagtgcctg gtcacgcttc
     2581 cattccaccg ggtcgatcgc gaagatccgc cgcgcccctg cgttcactgc gccctgcaac
     2641 gccgccatgc ctacgccgcc gatgccgacg atcgcgacgt cgtcaccagg gcggatatcg
     2701 gccgaccgcg tcgccgaacc gtagccggtg gtgacaccgc agccgaccag gcaagccacc
     2761 tcgaacggga tcgacgggtc gatcttcacc accgagctct tgtgcaccac catgtacggc
     2821 gagaaggtgc ccagcagcgt catcgggaag acgttctgac cgcgggcctg gatacgaaat
     2881 gtgccgtcgg acaccgccgc accgttgagc agaccagctc ccaggtcgca caggttgcgc
     2941 atcccggcct gacatgacgg acaccgcccg catgacggga tgaacgacag caccacgtgg
     3001 tcgccgggag cgatgtcctc gacaccgggg ccgacctcgg tgacgatgcc ggcaccctcg
     3061 tgtccgccga gtaccgggaa gccgcccatc gggattccgc cggtcaccag gtggtggtcg
     3121 gagtggcaca tccccgacgc ttccatctgg atcttgactt cgtccttgac cgggtcgccg
     3181 atctcgatct cttcgatcga ccacggctgg ttgaactccc agagaagagc gcccttggtc
     3241 ttcatcccag ttcctgcttc ccatgagaaa tcgtgtgaac tttcagcgac agcgtagtta
     3301 ccgaagtcac acaccaccca accgggtgtt tgggtcacca cccgcgcggg cgggggcaac
     3361 taccacagcg gcgcgggcgc cttgccgagc gggtaatagc cgggcagctt ctcgccggcg
     3421 aggctgcgct cgatccgctt ctgcatgccc tcgctgagct ttccggcctt catcaggtct
     3481 aggtagagct tctgcacatg gccgaagtcg aagaagtcgc gctgccattc gatcttgccg
     3541 ttggcggcga ggcgaaacca gctgccgccg atgccgtaga tctccgattc caccgcgtcg
     3601 gcgtccgtcg cgacctgttt ccagaagccg acgatctcgc cgatcttgtc gtcgatgatc
     3661 acgcgctgat acgggtagtg ccagttctcc aggccggcca tctcgaggcc gagcgcgatg
     3721 tcgcggatct cgtcgatgcc gacgcacatc acgtcttcct tcgggccgat gttccatccg
     3781 taggtggctt cgtcgtcgta gaagtcggcc agcggcgtcc agtcgccttt gcgctcggct
     3841 ttgcggttgg cctccagcca ccgctcgacc caatcttcca gttcctcacg ggggaatgac
     3901 gtcattgtca gtcttccttc tctcgaatgg acaacgcttg agtgggacag gcaaacacca
     3961 acgtaagagg cggttgtcaa gtggtctcgg gaatttttct ggctcgattg tccagcctgg
     4021 tcgggatggt tggtcggtcg gcgattcttg tcagtgggcg cgcgggaggc cgctggccgg
     4081 cctggccggc cagctcccgc gtggttgat
//
LOCUS       DASWBF010000110        15541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_339098,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000110 DASWBF010000000
VERSION     DASWBF010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15541
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339098"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="aceA"
                     /locus_tag="VF874_03785"
     CDS             complement(<1..635)
                     /gene="aceA"
                     /locus_tag="VF874_03785"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX9175239.1"
                     /translation="MSTVGKPKTPEQIQHDWDTNPRWKDTTRSYTAEDVVALQGTVVE
                     EQTLARRGAEVLWSQLHDLEWVNALGALTGNMAVQQVRAGLKAIYLSGWQVAGDANLS
                     GHTYPDQSLYPANSVPQVVRRINNALMRADEIAKVEGDRSVDNWLVPIVADGEAGFGG
                     ALNVYELQKAMIAAGVAGSHWEDQLASEKKCGHLGGKVLIPTQQHIRTLTSA"
     gene            complement(909..1703)
                     /locus_tag="VF874_03790"
     CDS             complement(909..1703)
                     /locus_tag="VF874_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEX9175240.1"
                     /translation="MSLDKVMMPVPDPHPDVFDREWPLRVADIDATGRLRLDAACRHI
                     QDIGADQLREMGFQDTHPLWIVRRTMIDLIRPIEFQDMLRLRRWCSGTSNRWCEMRVR
                     VDGRKGGLIESEAFWININRETQGPARIADDFLEGLHRTTDIDRLRWKAYLKPEPRDT
                     ADQIREFPVRFTDIDMFDHMNNAVYWSVVEDYLSATPELLRGPARITLEHEAPVALGD
                     KLEIVVHVHPPGSTVRFGEALAKKTVTTLTYMVGDDTKAVASIFSL"
     gene            1792..3210
                     /gene="ramB"
                     /locus_tag="VF874_03795"
     CDS             1792..3210
                     /gene="ramB"
                     /locus_tag="VF874_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate metabolism transcriptional regulator
                     RamB"
                     /protein_id="HEX9175241.1"
                     /translation="MPKTFVGSRVRQLRSERGFSQAALAQMLEISPSYLNQIEHDVRP
                     LTVAVLLRITEVFGVDATFFAPQDDTRLVAELREVTMDRDLDIDVHVAEVADMVSAHP
                     ALARAMVNLHRRYRITTAQLAAATEDRFSDGSSGSGSITMPHEEVRDYFYQRQNYLHE
                     LDTAAEELTIDLRMHRGDLARDLSDRLTAVHGVHIVKRLDLGDTVLHRFDPSSKRLEI
                     GNHLSSGQQVFKMATELAYLEYGDLIDKLVEEGKFTSEESTTLARLGLASYFAAATVL
                     PYEQFHEVAENFRYDIERLSAFYSVSYETVCHRLSTLQRPSMRGVPFSFVRVDKAGNM
                     SKRQSATGFHFSASGGTCPLWNVYETFANPSKILVQIAQMPDGRSYMWVARTVERRAS
                     RYGEPGKTFAIGLGCELRHAHRLVYSEGLDLSADNATPIGAGCRVCERDNCPQRAFPA
                     LGRALDLNEHRSTVSPYLVKPS"
     gene            3207..3740
                     /locus_tag="VF874_03800"
     CDS             3207..3740
                     /locus_tag="VF874_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9175242.1"
                     /translation="MTAPRIEPGGFRELGPITWAFTKLAARVARVPQVHLFTTLGQHK
                     RLFWTWLPFGAVLLGFGKLPRRDSELVILRVGHLRGCEYQLQQHRRIAQRRGIDAETQ
                     DKIFEGPNADGLTDRERALLTATDELIDARNVSAQTWQWLARHLDRRQLIEFVTLAGQ
                     YDALAATLTTLKVPLDL"
     gene            complement(3737..5134)
                     /gene="lpdA"
                     /locus_tag="VF874_03805"
     CDS             complement(3737..5134)
                     /gene="lpdA"
                     /locus_tag="VF874_03805"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9175243.1"
                     /translation="MTSHYDVVVIGAGPGGYVAAIRAAQLGLNTAIVEPKYWGGVCLN
                     VGCIPSKSLLRNAELAHIFTKQAKTFGISGEATFDYGVAFDRSRKVADGRVAGVHFLM
                     KKNKITKIHGYGKFKDANTLEVDLNDGGTETVTFDNAIIATGSSTKLVPGTSLSENVV
                     TYEEQILSRELPGSIVIAGAGAIGMEFGYVLANYGVDVTIVEFLPRALPNEDAEVSKE
                     IEKQFKKLGVKVLTGTKVESIDDDGSTVTVKVSKDGNSDELKADKVLQAIGFAPNVEG
                     YGLDAAGVKLTDRKAIAIDEQMRTNVDHIYAIGDVTGLLQLAHVAEAQGVVAAETIAG
                     AETLELGDYRMMPRATFCQPQVASFGLTEEQARDEGYDVKVAKFPFTANGKAHGLGEP
                     TGFVKLIADGRYGELIGGHLIGPDVSELLPELTLAQKWDLTANELARNVHTHPTLSEA
                     LQECFHGLVGHMINF"
     gene            complement(5203..5679)
                     /locus_tag="VF874_03810"
     CDS             complement(5203..5679)
                     /locus_tag="VF874_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEX9175244.1"
                     /translation="MADAPPRVLITETAAGLLRRLRDRHGALMIHQSGGCCDGSSPMC
                     YPDGEFIVGDRDVLLGIVEDTPVWISGPQFDAWKHTQLVIDAVPGRGGGFSMEAPEGM
                     RFLSRGRAFTAAELESLAGAPPLRGAEYASGERPAPPDGPVVVAEAVDACPVPRGR"
     gene            complement(5679..7202)
                     /gene="adh"
                     /locus_tag="VF874_03815"
     CDS             complement(5679..7202)
                     /gene="adh"
                     /locus_tag="VF874_03815"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX9175245.1"
                     /translation="MTVFARPGTEGSLISFDSRYGNFIGGEWVPPVGGEYFENPTPVT
                     GQPFCEVPRSTEADIDKALDAAHAAAPEWGKTAAAERALVLNKIADRIEENLEAIAVA
                     EAWDNGKPVRETLNADIPLVVDHFRYFAAAIRAQEGTLSQIDEDTVAYHFHEPLGVVG
                     QIIPWNFPILMATWKLAPALAAGNAVVLKPAEQTPASILYLLGLIGDLLPAGVVNVVN
                     GFGFEAGKPLASSNRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFFSDV
                     LAAADDFQDKALEGFTMFALNQGEVCTCPSRSLIQADIYDEFLELAAIRTKAVRQGDP
                     LDTETMIGSQASNDQLEKILSYVEIGKDEGARVVTGGERAELGGDLSGGYYMQPTIFE
                     GNNKMRIFQEEIFGPVVAVTSFRDYDDAIAIANDTLYGLGAGVWSRDGTTAYRAGRDI
                     KAGRVWTNCYHAYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNLLVSYNDKAQGFF"
     gene            7285..9309
                     /locus_tag="VF874_03820"
     CDS             7285..9309
                     /locus_tag="VF874_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9175246.1"
                     /translation="MGSPDSDPYLWLEDVTGEAALGWVRRHNEPTLAELCDGRFEEFR
                     EQALDVLDTDARIPYVVRRGDHLYNFWRDAEHPRGVWRRTTLEQYRSESPDWDVLIDV
                     DALAAQEDENWVWAGANVIEPDFSLALISLSRGGADAAVEREFDMATREFVADGFVVP
                     EAKTAIDWEDPDTVLIGTDFGDGSLTDSGYPRLVKRWRRGRPLSDAELLFSGSHVDVS
                     VAASVDRTPGFERTLVRRSIDFFNSDVYELRRGEFIRIDAPTDATVSIHRQWLLIELR
                     SDWYTATTHYRAGSLLAADYDEFISGTAQLHVVFEPDEHTCLHHYAWTRDRLVVVTLA
                     DVASRVEVVKPGDWRAEPVTGLPENTNTVIVAADHAGDEVFLDSSGFDRPSRLMRGIA
                     GGELEQIKSAPQFFDADDLTVAQHFAISEDGTQVPYFVVGHGANGPGPSLLGGYGGFE
                     VSRTPAYDGVMGRLWLARGGTYALANIRGGGEYGPTWHTQATREGRHKVAEDFAAVAQ
                     DLVDRGITTVAQLGAAGGSNGGLLMGIMLTQYPELFGALVCSVPLLDMRRFHLLLAGA
                     SWVAEYGNPDDPADWEFIEKFSPYHHVSADRHYPPLLITTSTRDDRVHPGHARKMAAA
                     LQAAGHHVRYYENIEGGHAGAADNKQAAFKSALMFEFLWRTVGSAPPRSS"
     gene            complement(9242..10117)
                     /locus_tag="VF874_03825"
     CDS             complement(9242..10117)
                     /locus_tag="VF874_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175247.1"
                     /translation="MRSVAMVLCWLVATVALAVAIPAGWAQRNFIDNDGYAALASSAA
                     RDLALQNAMASLLTTQTLTFAGDRGYNFPEPVVRGVVTNYIASPSFPAQFADANRLAH
                     RWLFTDTVQQSGDRWTIDVAPMLSNLSTIQTLNARGVPLPSSVRVPLTSGTVQTLRPG
                     ELQPLATWGPLVSAGAAVLAAVAALLTVAVAKARGKALAALGVSALLVGGGGWAAIEI
                     GRRHIDDALNQTTDDVRTIGDVMVSHAEGSLHHWLNLTLAAGGALVVVAVVVTMIGAA
                     RNRPSATETRTSAQI"
     gene            complement(10129..11652)
                     /locus_tag="VF874_03830"
     CDS             complement(10129..11652)
                     /locus_tag="VF874_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9175248.1"
                     /translation="MTATSDVKPSTTGTIFNPATGDVAGEVHWTDPADVPRIAARLAQ
                     NQRDWEQRGATGRAKVLARFAVWLGEHRDEIEDLLIKETGKSRTDAVQEVPMLVMITS
                     YLIKTMERALAPETRPAPLPALAVKKITVHFRPRPVVGIIAPWNFPVANAMMDALGAL
                     AAGCAVLLKPSERTPLTAEILVRGWRESGAPDVFALAQGAREAAEAVIDVSDFVHFTG
                     STATGRKVMERATRRLTPVSLELGGKDPMIVLADADVDLAAHAAVWGGLFNAGQMCVS
                     VERVYVQDSVYDKFVDAVVRDVKALQVGAGDGPHFGSMIDEQQLAIVERHVANAKAKG
                     ARVLTGGERVPGPGSFYQPTVLVDVDHSMECMTEETFGPTLPIMKVASVSEAVKLAND
                     SPYGLSASVFSTDIERAKEVALQIDCGAVNINDVVANLMCTTAPMGGWKESGIGARFG
                     GAEGMRKYCRMETVVEPRFKVGSGVNYYSSSPKAATRMSKQMMWMALRRPKRVARDR"
     gene            complement(11657..12217)
                     /locus_tag="VF874_03835"
     CDS             complement(11657..12217)
                     /locus_tag="VF874_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol transferase"
                     /protein_id="HEX9175249.1"
                     /translation="MPDTDAAAAQELLRDSFTRLIEHVEALTDGLTDELATYQPTPQA
                     NSIAWLIWHSARVQDVQVCDIAKTLSDGRPDKPRSVVGPGDVWTRDGWVDRFGLDLPR
                     DDTGYGHSPDDVAKVRASADLLKGYYRAVHDMTLEFVTTLSPADLARIIDRNWDPPVT
                     ASARLVSIIDDCAQHLGQAAYVRGIA"
     gene            complement(12228..12629)
                     /locus_tag="VF874_03840"
     CDS             complement(12228..12629)
                     /locus_tag="VF874_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEX9175250.1"
                     /translation="MNAPQPPYGSENPYQQPQGQYGPPPGGAYPPQPYQGQPAPYAAP
                     GAHPGALWGVDQYGRPLSDKSKVTAGLLSLFLGGFGVGRFFLGYTTFGILQIVITVVT
                     IGTLGWVWPLIDTILIFTGNLTDAQGRTLRD"
     gene            12717..13022
                     /locus_tag="VF874_03845"
     CDS             12717..13022
                     /locus_tag="VF874_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9175251.1"
                     /translation="MDAFAVIADPTRRRILDTLRDGGADVGTLVERLGISQSLVSKHL
                     GVLRDAGTVQVEVAGKRRVYHLADDPLPAVLAWITPYQRKWAASLDRLTRLIDEEQS"
     gene            13019..13519
                     /locus_tag="VF874_03850"
     CDS             13019..13519
                     /locus_tag="VF874_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9175252.1"
                     /translation="MSHPLQATLEDRDGRWVLSFTRDLGHPVEAVWPWLTEPERLRRW
                     SPVVPDRRFDSAGPAEVRENPGDDPVDGTVVTVDAPRELVHRWGPDLLRWRLEPTETG
                     CRLTLEHQMAERDHEAENAGGWHICLDVLASNLDGANPERLVGEVVMDHGFPELRDGY
                     RKLLGV"
     gene            complement(13516..13887)
                     /locus_tag="VF874_03855"
     CDS             complement(13516..13887)
                     /locus_tag="VF874_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9175253.1"
                     /translation="MIDHLGINCSDFGKAKEFYDRVLGVLGFTRQMDFGVAIGYGKDG
                     KPDFWIADADLGPNREIHVAFQAADEGAVRAFYDAAIELGAESLHAPRLWPEYHPGYF
                     GAFVRDPDGNNVEAVFHGANP"
     gene            13918..>15541
                     /locus_tag="VF874_03860"
     CDS             13918..>15541
                     /locus_tag="VF874_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9175254.1"
                     /translation="MSYDLVIRGGTIVDGLGGEPFVGDVAVSDGVIVAVGTVDGSAAR
                     EIDASGLLVTPGFVDLHTHYDGQAIWSDRLTPSSRHGVTTAVMGNCGVGFAPCRADDH
                     DVLVDVMAGVEDIPGVVMADGLPWDWETFPEYLNALDARHRDIDVAAYLPHSPLRVYV
                     MGQRGVDREPATADDLARMRALAKEAIEVGAVGFASSRFVMHKTAGGQQIPTYNAACE
                     EISAIAQGVADGGGGLLQFVPDIPAGGYESVLGQVFEVAQDVGLPVTFSMATGNSGDP
                     VWPDAFTLIEKFTSAGVDITAQMFPRPIGLVIGLELSGNPFILYPSYRSIADLPLAER
                     VAEMRKPQVRERILADKPTAEGNPLLYLAQMWQWMYPLGDTPDYEPSASTSISARAAA
                     RGVTPQEEAYDRLLDDDGHAMLLVAMGNYQDNSLDTVGELLRRDDVVLGLGDGGAHYG
                     MICDASFPTFLLTHWARDRAHGRMSVAEAVRELTSVPARVAGLADRGRIALGYKADLN
                     VIDHAGLRLHRPVITYDLPAGGRRLDQTAEGYV"
BASE COUNT         2552 a         5181 c         5110 g         2698 t
ORIGIN      
        1 gcggacgtca gcgtgcggat gtgctgctgg gtcgggatca gcaccttgcc gcccaggtga
       61 ccacacttct tctccgacgc cagctgatcc tcccagtggg aaccggccac gcctgcggcg
      121 atcatcgcct tctgcagctc gtagacgttg agtgcaccac cgaagccggc ctcgccgtcg
      181 gcgacgatcg gcaccagcca gttgtcgacg gacctgtcgc cctcgacctt cgcgatctcg
      241 tcggcgcgca tcagcgcgtt gttgatccga cgcaccacct gcggcaccga gttggccgga
      301 tacaggctct ggtccggata ggtgtgcccg gacaggttcg cgtcaccggc gacttgccaa
      361 cccgaaaggt agatggcctt caggcccgcg cggacctgct gcacggccat gttgccggtc
      421 agcgcaccga gcgcattcac ccattcgagg tcgtgcagct gcgaccacag cacctcggcg
      481 ccgcggcggg cgagcgtctg ctcctcgacc acggtgccct gcagcgcgac gacgtcctcg
      541 gcggtgtagc tgcgggtggt gtccttccag cggggattgg tgtcccaatc gtgctggatt
      601 tgctccggcg tcttgggctt gccgacggtc gacatttgag atgctccttc gaagttgctg
      661 acaagccgct gccgcgtcgc cggcttcccg agttgttaac acgacacata gcttcgctgc
      721 tgtgctaact cgaccatgac acagcgtata tgcgcaggtc caggggtttg ctttgccaag
      781 ttctgctact agccaggttg cttttgcaaa tcttgcaaag ctagctaatt gctgtaccgg
      841 ttcagttggc tactcaccgg taaccttatt cacgcaggtc aagtgcgcaa acgagccgga
      901 tctgatcttt acagtgagaa gatcgaagcg acggccttcg tatcgtcgcc aaccatgtat
      961 gtgagcgttg taacagtctt ctttgcaagc gcttcgccga agcggaccgt cgatcccggc
     1021 gggtgcacgt gcacgacgat ctccaacttg tcgcccagcg cgaccggcgc ctcgtgctcg
     1081 agagtgatcc gggccgggcc gcgaagaagt tccggcgtgg ccgagagata gtcctcgacg
     1141 acgctccagt acaccgcgtt gttcatgtgg tcgaacatgt cgatgtcggt gaaccggacc
     1201 gggaactcgc ggatctgatc agcggtgtcc cggggctccg gcttcaggta cgccttccac
     1261 cgcagccgat cgatatcggt ggtgcggtgc agcccctcga ggaagtcgtc ggcgatgcgc
     1321 gccgggccct gggtctcgcg gttgatgttg atccagaatg cctcggactc gatcagcccg
     1381 cccttgcggc cgtcgacgcg aacccgcatc tcacaccagc ggttcgatgt ccccgaacac
     1441 caccgccgca gccgcagcat gtcctggaat tcgattggcc ggatcaggtc gatcatcgtg
     1501 cggcgcacga tccacaacgg gtgggtgtcc tggaaaccca tctcgcgcag ctggtccgcg
     1561 ccgatgtcct ggatgtgtcg gcacgcggca tccagccgca gccggccggt ggcgtcgatg
     1621 tcggcgaccc gcaacggcca ctcccggtcg aagacatcgg gatgcgggtc gggcaccggc
     1681 atcatcactt tgtccaggct cattgaagcg cgatcatgcc aaacggcgac gccaacggcc
     1741 aactgccaag actgcaaagc ggcggttagc acggcaagta ggctgctcag catgccgaag
     1801 acgttcgtcg gatcacgggt ccgtcagctg cgaagcgaac ggggcttcag ccaggcggcg
     1861 ctggctcaga tgctggagat ctcaccgagc tacctgaacc agatcgagca cgacgtgcgg
     1921 ccgctgaccg tggcggtgct actgcggatc accgaggtgt tcggcgtcga cgcgacgttc
     1981 ttcgccccgc aggacgacac ccggctcgtc gccgaactgc gcgaggtgac catggaccgc
     2041 gacctcgaca tcgacgtcca cgtcgccgag gtggccgaca tggtcagcgc ccatcccgcg
     2101 ctcgcccggg cgatggtcaa cctgcaccgt cgctaccgga tcaccaccgc gcagctggcg
     2161 gcggccaccg aggaccgctt cagcgacggc agcagcggca gcggatcgat cacgatgccg
     2221 cacgaggagg tccgcgacta cttctatcag cgccagaact atctgcacga gctcgacacc
     2281 gccgctgagg aactcaccat cgatctgcgc atgcaccgcg gtgaccttgc ccgcgatctt
     2341 tccgaccgtc ttacggccgt gcacggcgtg cacatcgtca agcggctcga tctcggcgat
     2401 accgtgctgc atcgcttcga cccgtcgtcc aagaggctgg agatcggcaa ccacctgtcg
     2461 tcaggccagc aggtgttcaa gatggcgacc gaactggcat acctcgaata cggcgacctg
     2521 atcgacaagc ttgtggagga gggcaagttc accagcgagg agtcgacaac cctggcccgg
     2581 cttggtcttg ccagctactt cgccgcagcg acggtgttgc cctacgagca gtttcacgag
     2641 gtcgcggaga atttccgcta cgacatcgag cggctgtcgg cgttctactc ggtgagctac
     2701 gagacggtct gccaccggct ttccacgctg cagcggccgt cgatgcgcgg cgtgccgttc
     2761 tcgttcgtac gggtcgacaa ggcgggcaac atgtcaaaac ggcagtccgc taccggtttt
     2821 cacttttcgg ccagcggcgg cacctgcccg ctgtggaacg tctacgagac tttcgccaat
     2881 ccgagcaaga ttctcgtgca gatcgcccag atgcctgacg gtcgcagcta catgtgggtg
     2941 gcacgcacgg tcgagcggcg cgcctcacgc tatggtgagc cgggtaagac gttcgcgatc
     3001 ggcctgggtt gcgaactacg gcatgcccac cggctggtgt attcggaggg actggacttg
     3061 tcggctgaca acgccacacc gatcggcgcc ggctgccgcg tctgcgagcg ggacaactgt
     3121 ccgcagcgcg cgttcccggc gctgggccgc gccctcgatc tcaacgagca ccgcagcacg
     3181 gtgtcgcctt acctggtgaa gccgtcatga cagctccccg catcgagccc ggcggcttcc
     3241 gcgagctcgg cccgatcact tgggcgttca ccaaactcgc ggcccgggta gcccgcgtgc
     3301 cgcaggtgca tctgttcacc acgctcggcc agcacaagcg gttgttctgg acgtggctgc
     3361 ccttcggcgc ggtcctgctc gggttcggca agttgccgcg ccgcgacagc gagctggtga
     3421 tcctgcgggt cggacacctg cgcggctgcg agtaccagct gcagcagcat cgccgtatcg
     3481 cccagcgccg cggcatcgac gcagaaacac aggacaagat cttcgaaggc ccgaacgccg
     3541 acgggttgac cgaccgcgag cgggccctgc tgaccgccac cgacgaactc atcgacgccc
     3601 gcaacgtgtc cgcacaaaca tggcagtggt tggcgcgtca cctcgatcgt cggcagttga
     3661 tcgaattcgt cactctcgca gggcaatacg acgcactcgc ggcgacgttg accacgctga
     3721 aggtgccgct tgacctctag aagttgatca tgtggccgac gagcccgtgg aagcactcct
     3781 gtagcgcctc ggacagcgtc gggtgggtgt gcacgttgcg ggcgagctcg ttggccgtca
     3841 ggtcccactt ctgtgccaac gtcagctccg gcagcagctc ggacacgtcg ggcccgatca
     3901 ggtgtccgcc gatcaactcc ccgtagcggc cgtcggcgat cagcttgacg aacccggtcg
     3961 gctcccccag cccgtgcgcc ttgccgttcg ccgtgaacgg gaacttcgcg accttgacgt
     4021 cgtaaccctc gtcgcgggcc tgctcctcgg tcagcccgaa gctggcgacc tgcggctggc
     4081 agaacgtcgc ccgcggcatc atccggtagt caccgagctc caaagtctcc gcgccggcga
     4141 tggtttcggc cgcgaccacg ccttgtgcct cggcgacgtg cgcgagttgc agcaggccgg
     4201 tgacgtcgcc gatcgcgtag atgtggtcga cgttggtgcg catctgctcg tcgatcgcaa
     4261 tcgctttgcg gtcggtcagt ttcaccccgg cggcgtccag gccgtagccc tcgacgttgg
     4321 gcgcaaagcc gatggcctgc aacaccttgt cggccttcag ctcgtcggag ttgccgtcct
     4381 tgctgacttt gacggtgacc gtcgacccat cgtcgtcgat cgactcgacc ttggttccgg
     4441 tgagcacttt cacgccgagc ttcttgaact gcttctcgat ttccttggac acctcggcgt
     4501 cctcattggg cagtgcccgc ggcaggaatt cgacgatcgt cacgtcgacg ccgtagttgg
     4561 ccagcacgta accgaactcc atcccgatcg cgccggcccc ggcgatcacg atcgagccgg
     4621 gcaactcgcg ggacaggatt tgctcctcgt aggtgacgac gttctccgac agcgacgtgc
     4681 cgggcaccag cttggtgctg ctgccggtcg cgatgatcgc gttgtcgaag gtgacggtct
     4741 cggtgccgcc gtcgttgagg tcgacttcca gcgtgttggc gtctttgaac ttcccgtagc
     4801 cgtgaatctt ggtgatcttg ttcttcttca tcaggaagtg cacgccggcg acgcggccgt
     4861 cggcgacctt gcggctgcgg tcgaacgcga cgccgtagtc gaacgtcgcc tcaccgctga
     4921 tcccgaacgt cttggcctgc ttggtgaaga tgtgggccaa ctctgcgttg cgcagcaacg
     4981 acttcgacgg gatgcagccg acgttgaggc agactccgcc ccaatacttg ggttcgacga
     5041 tggcggtgtt caggcccagt tgggcggcgc ggatggccgc gacgtatccg ccggggcccg
     5101 ctccgataac gacgacgtcg tagtgagaag tcacgaggcc agcctagtaa gggacgacgc
     5161 ctaccaggca agggctcgcg atccggtcgc agaagtgccg agttagcgac cccgcggcac
     5221 cgggcaagca tctaccgcct cggccaccac cacaggcccg tcaggagggg ctggccgttc
     5281 cccgctcgcg tactccgcgc cccggagcgg cggcgcaccg gcgagtgact cgagctccgc
     5341 ggcggtgaac gcgcggcccc ggctgaggaa ccgcatgcct tcgggggctt ccatcgagaa
     5401 cccaccgccg cggccgggca ccgcgtcgat gaccagctgg gtgtgcttcc acgcgtcgaa
     5461 ctgaggacct gaaatccaca ccggcgtgtc ctcgacgatg ccgagaagca cgtcccggtc
     5521 gccgacgatg aactccccgt ccgggtagca catcggcgac gatccgtcgc agcagccgcc
     5581 ggactggtga atcatcaacg cgccgtggcg atctcgcagc cgacgcagca ggccggcggc
     5641 cgtctcggtg atcagcaccc gcggtggggc gtctgccatc agaagaagcc ctgcgccttg
     5701 tcgttgtagg agacgagcag gttcttggtc tgctggtagt ggtcgagcat catcttgtgg
     5761 ttctcgcgcc cgatgccgga ctgcttgtag ccgccgaacg ccgcgtgcgc ggggtaggcg
     5821 tgatagcagt tggtccacac ccggccggcc ttgatgtcgc gcccagcccg ataggcggtg
     5881 gtgccgtcgc ggctccacac ccccgcaccc agcccgtaca gggtgtcgtt cgcgatcgcg
     5941 atcgcgtcgt cgtagtcacg gaacgacgtg accgcgacga ccgggccgaa gatctcctcc
     6001 tggaagattc tcatcttgtt gttgccctcg aagatcgtcg gctgcatgta gtagccaccg
     6061 gacaggtcgc cgccgagttc ggcgcgttcg ccgccggtga cgacgcgcgc gccctcgtcc
     6121 ttgccgatct cgacgtagga caggatcttc tccagctggt cgttggaggc ctgcgaaccg
     6181 atcatcgtct cggtgtccag cgggtcgccc tggcggacgg ccttggtgcg gatcgcggcg
     6241 agctcgagga attcgtcgta gatgtcggcc tggatcaggc tgcgcgacgg gcacgtacac
     6301 acctcgccct gattcagcgc gaacatcgtg aacccttcca gcgccttgtc ctggaagtcg
     6361 tcggcggcgg ccagcacgtc ggagaagaag atgttggggc tcttgccgcc cagctccagc
     6421 gtgaccggga tgaggttctg gctggcgtac tgcatgatca gccgcccggt ggtggtctcc
     6481 ccggtgaacg cgatcttggc gatccggttg ctggaggcca gcggcttgcc cgcttcgaac
     6541 ccgaacccgt tgaccacgtt gaccacgccg gccggcagca gatccccgat caggcccagc
     6601 aggtacagga tcgaggccgg ggtctgctcg gccggcttga gcacgaccgc gttgccggcc
     6661 gccagcgcgg gcgcgagctt ccaggtcgcc atcaggatcg ggaagttcca cgggatgatc
     6721 tgcccgacca cccccagcgg ctcgtggaag tgatacgcga cggtgtcctc gtcgatctga
     6781 ctcagcgtcc cctcctgggc gcggatcgcc gccgcgaaat agcggaagtg gtccaccacc
     6841 aacggaatat cggcgttgag ggtttcccgc accggcttgc cgttgtccca tgcctcggcg
     6901 acggcgatgg cctcgaggtt ttcttcgatg cggtcggcga tcttgttcag caccagcgcg
     6961 cgctccgccg ccgccgtctt cccccactcg ggcgccgccg cgtgcgcagc atccaacgcc
     7021 ttgtcgatgt cggcctcggt cgaccgcggc acctcacaga acggctgtcc cgtgaccggc
     7081 gtcggattct cgaaatattc gccgcccacc ggcgggaccc actcgccgcc aatgaaattg
     7141 ccgtaacgcg aatcgaacga tatcaacgaa ccctcggtac ccggacgggc aaacacagtc
     7201 atcagcgact ccttcgtcgt ggcgcgtgta ctacggctca cagtactccc ggcgccgaca
     7261 acccgaccct gtaagaatgc ccgggtgggc agccccgata gcgacccgta cctctggctc
     7321 gaggacgtca cgggcgaggc tgcgctgggc tgggtgcgcc ggcacaacga accgacgctg
     7381 gccgagctgt gtgacggacg ctttgaggag ttccgcgagc aggcgctgga cgtgctggac
     7441 accgacgccc gcatcccgta cgtcgtgcgg cgtggcgatc acctgtacaa cttctggcgc
     7501 gacgccgagc acccgcgcgg tgtctggcga cggacgacgc tcgagcagta ccgcagcgag
     7561 tcaccggact gggatgtgct gatcgacgtc gacgcattgg ccgcacagga ggacgagaac
     7621 tgggtgtggg ccggtgccaa cgtcatcgaa ccggatttct cgctggcgtt gatctcgctg
     7681 tcgcggggcg gcgccgacgc ggcggtggag cgcgaattcg acatggcgac aagggagttc
     7741 gtcgccgacg ggtttgtcgt tccggaggcc aagaccgcca tcgactggga agaccccgac
     7801 accgtgctca ttggcaccga tttcggcgac gggtcgctga cggactccgg ctatccccgg
     7861 ctcgtcaagc gctggcggcg cggccggccg ctgtccgacg ccgagctgtt gttctccggc
     7921 agccacgtcg acgtcagcgt cgcggcgtcc gtcgaccgca cccctggttt cgagcggaca
     7981 cttgtgcgcc ggtctatcga cttcttcaac agtgacgtgt acgaactgcg ccgcggcgag
     8041 ttcatccgga tcgatgcacc caccgacgcc acggtgtcga tacaccggca gtggctgctc
     8101 atcgaactgc gcagcgactg gtacaccgcc accacccact accgcgccgg ctcgttgctg
     8161 gcagcggact acgacgaatt catttccggc acagcgcaat tgcacgtagt gttcgaaccg
     8221 gacgagcaca cctgcctgca tcactacgcg tggacacgcg accggctggt ggtggtcacc
     8281 ctcgctgacg tggcgagccg tgtcgaggtc gtgaagcctg gggactggcg ggctgagccg
     8341 gtcaccggcc tgccggaaaa caccaacacc gtgatcgttg ccgccgacca cgccggcgac
     8401 gaagtctttc tcgactccag tggtttcgac aggccgtcgc gtctgatgcg cggcatcgcg
     8461 ggcggcgagc tggagcagat caagtcggcg cctcagttct tcgacgccga cgacctcacg
     8521 gtcgcacagc atttcgcgat ctccgaggac ggcacacagg tgccgtactt cgtcgtcgga
     8581 cacggcgcca acggccccgg tccctcgctg ctgggcggct atggcggatt cgaggtctcg
     8641 cgcacaccgg cttacgacgg cgtgatggga cggctgtggc tggcccgcgg cggaacctat
     8701 gcgctcgcca acatccgcgg cggcggcgag tacggaccga cgtggcacac gcaggcaacg
     8761 agggagggcc ggcacaaggt cgccgaggac ttcgccgcag ttgcacagga cttggtggac
     8821 agaggcatca ccaccgtcgc acagctggga gcggccggcg gcagcaacgg cgggctgttg
     8881 atgggcatca tgctcacgca gtacccggag ttgttcggcg ccctggtgtg cagcgtgccg
     8941 ctgctcgaca tgcgccgctt ccacctgctg ctcgccggcg cgtcgtgggt ggccgagtac
     9001 ggcaaccccg acgacccagc ggactgggag ttcatcgaga aattctcgcc ataccaccac
     9061 gtttcggccg atcggcacta tccgccgctg ctgatcacca cgtcgacccg cgacgaccgg
     9121 gtgcaccccg gacacgcgcg gaaaatggcc gccgcgctgc aggcggccgg ccaccacgtg
     9181 cgctactacg agaacatcga gggcggccac gccggtgccg ccgacaacaa acaggccgcg
     9241 ttcaaatctg cgctgatgtt cgagtttctg tggcggacgg tcggttccgc gccgccccga
     9301 tcatcgtgac gacgacggcg acaaccacca gcgcgccacc cgccgccaac gtcaggttca
     9361 gccagtggtg caggctgcct tcggcgtggc tgaccatcac gtcaccgatg gtgcggacgt
     9421 cgtcggtggt ctggttcagc gcgtcgtcga tgtgacggcg accgatctcg atcgccgccc
     9481 agccgccgcc gcccacaagc agtgccgaaa cacccaacgc agcaagggct ttgccacgtg
     9541 ctttcgcgac ggcgacggtc agcaacgcgg cgacggcggc caacaccgcg gcgccagcgc
     9601 tgaccaatgg tccccaggtg gccaagggct gcagttctcc cggccgcagg gtttgcaccg
     9661 tgccggacgt cagcggcacc ctgacgctcg acggcagcgg aaccccgcga gcattcagcg
     9721 tctgaatggt cgacaggttc gacagcatcg gggccacgtc gatagtccat cggtcgccgc
     9781 tctgctgcac cgtatctgtg aataaccagc gatgcgccag ccggttggcg tcggcgaact
     9841 gagcgggaaa cgacgggctg gcaatgtagt tcgtcacgac cccgcgcacc acgggttcgg
     9901 gaaagttgta gccgcgatct ccggcgaatg tgagcgtctg cgtggtgagc agcgacgcca
     9961 tcgcgttctg cagcgccaga tcgcgcgcgg ccgacgacgc cagcgcggcg tacccgtcgt
    10021 tgtcgatgaa gttgcgctgg gcccagccgg ccggaatcgc gaccgccaac gcgacggtgg
    10081 cgacgagcca gcacagcacc atcgccaccg aacgcacggc attagacctt agcggtcgcg
    10141 cgcgactcgc ttcggccggc gcagagccat ccacatcatc tgcttggaca ttcgggtcgc
    10201 ggccttcggg gacgacgagt agtagttgac gccggacccc accttgaagc gcggctcgac
    10261 gaccgtctcc atccggcagt actttcgcat tccctcggca ccgccgaagc gcgcgccgat
    10321 gccggattcc ttccagccgc ccatcggcgc cgtcgtgcac atcaaattcg cgaccacgtc
    10381 gttgatgttc acagcgccgc agtcaatctg cagcgcaacc tctttggcac gctcgatgtc
    10441 ggtcgagaac actgatgccg acagcccgta cgggctgtcg ttggcgagct tgaccgcttc
    10501 tgagacgctg gccaccttca tgatcggcag ggtcgggccg aaggtctcct cggtcatgca
    10561 ctccatcgaa tggtcgacgt cgacgagcac ggtcggctga tagaagctgc cgggaccagg
    10621 cacccgctcg ccgccggtaa gcacccgcgc gcctttggct ttcgcgttgg cgacgtgacg
    10681 ctccacgatc gccagctgct gttcgtcgat catcgacccg aagtgcgggc cgtcgccggc
    10741 gccgacctga agtgctttca cgtcgcgcac caccgcgtcg acgaacttgt cgtagacgga
    10801 gtcctgcacg tagacgcgtt cgaccgacac gcacatctgg ccggcgttga acagcccgcc
    10861 ccacaccgcg gcgtgcgcgg ccaggtcgac gtcggcgtcg gcgagcacga tcatcgggtc
    10921 cttgccgcca agctcgaggc tcaccggggt cagccggcgc gtggcgcgtt ccatcacctt
    10981 gcgcccggtc gccgtcgagc cggtgaagtg cacgaagtca ctgacgtcga tgacggcctc
    11041 ggcggcctct cgtgcaccct gcgccaacgc gaacacgtcg ggcgcaccgg attcccgcca
    11101 gccccgcacc agaatctcgg ccgtcagcgg cgtccgctcc gacggcttca gtagcaccgc
    11161 gcaaccggcg gccagcgcac cgagcgcgtc catcatcgcg ttggcgacag ggaagttcca
    11221 tggcgcgatg atcccgacga ccggacgcgg ccggaagtgc acggtgatct tcttgaccgc
    11281 cagtgccggc agcggcgccg gccgggtctc gggcgccagc gccctctcca tggtcttgat
    11341 caagtaggac gtgatcatca ccagcatcgg cacttcctgg acggcgtcgg tgcgcgactt
    11401 gccggtctcc ttgatcagca ggtcttcgat ctcgtcgcgg tgctcaccaa gccacaccgc
    11461 gaacctggcc agcaccttgg ctcggcccgt ggcgccccgc tgctcccagt cgcgctggtt
    11521 ctgggccagg cgggcggcga tccgggggac gtcggcgggg tcggtccagt gcacctcgcc
    11581 ggcgacgtcg ccggttgcgg gattgaagat cgtgcccgtc gtcgacggct tgacatctga
    11641 tgtggcggtc atcacctcat gctatgccgc gcacgtacgc cgcctgcccg aggtgctgcg
    11701 cgcagtcgtc gatgatgctg accagccgcg cgctcgcggt gaccggtgga tcccagttcc
    11761 ggtcgatgat ccgcgccaag tcggcgggac tcagcgtcgt gacgaactcg agcgtcatgt
    11821 cgtgcaccgc gcggtagtag cccttcagca ggtcggccga cgcccggacc ttcgccacgt
    11881 cgtcggggct gtgcccgtag ccggtgtcgt cgcgcggaag gtccaagccg aaccggtcca
    11941 cccagccgtc gcgcgtccag acgtcccccg gaccgaccac ggagcgaggc ttgtcggggc
    12001 gaccatcgga caatgtcttg gcgatgtcac agacctggac atcttgcacc cgcgcgctgt
    12061 gccagatcag ccaggcgatg ctgttggcct gcggggtggg ttggtaggtc gcgagctcgt
    12121 cggtgagtcc gtcggtcagg gcctcgacgt gttcgatcag acgggtgaac gagtcgcgga
    12181 gcagttcctg cgcggcggcg gcatcggtgt cgggcatgca accgaggcta atcgcgcaac
    12241 gtgcggccct gggcgtcagt cagattgccg gtgaagatca ggatcgtgtc gatcagcggc
    12301 cagacccacc ccagcgtgcc aatcgtcacc acagtgatca caatctgcag gatgccgaag
    12361 gtcgtgtatc ccagaaagaa gcggccgacg ccgaatccgc cgaggaaaag gctcagcaat
    12421 cccgccgtga ccttgctctt gtccgacaac ggacgcccgt actggtcgac gccccacagc
    12481 gcgcccgggt gagcgccagg cgccgcgtac ggtgctggct gtccttgata cggctgcggt
    12541 ggatatgcgc cgcccggcgg cggtccgtat tgaccttgcg gttgctgata ggggttttcg
    12601 gagccgtacg gcggctgagg cgcattcaca aggagatgat gtcccaggcg tcgcccggcg
    12661 tccacagaac gaacatatga cttgaatttc atatgattat ttggtgatat gtcggggtgg
    12721 acgcgttcgc ggtgatcgcc gaccccaccc ggcgacgcat tctggacacc ctgcgcgacg
    12781 gtggtgccga cgtcggcaca ttggttgagc gactggggat ttcgcagtct ctggtatcca
    12841 aacacctcgg ggtgctccgc gacgcgggca cggtacaagt cgaggtggcc ggcaagcggc
    12901 gcgtgtacca cctggccgac gacccgctgc cagccgtgct cgcgtggatc acgccgtatc
    12961 aacgcaagtg ggccgccagc ctggaccggt tgacccgatt gatcgacgag gagcagtcat
    13021 gagccatccc ctgcaagcca cgctcgaaga ccgcgacgga cgctgggtgt tgtcgttcac
    13081 ccgcgacctc ggccacccag tcgaagcggt gtggccgtgg ctgaccgaac cggagcggct
    13141 gcggcgatgg tcgccggtgg taccggaccg cagattcgac agcgccgggc ccgcggaggt
    13201 ccgggagaac cccggcgacg atcctgtcga cggcacagta gtgaccgtcg atgcgcctcg
    13261 tgagctcgtg catcggtggg gtcccgacct gctgcggtgg cgtctcgagc cgaccgagac
    13321 cggatgccgg ctcaccctgg agcaccagat ggcggagcgg gaccatgagg ccgagaacgc
    13381 cggcggctgg cacatctgcc tggacgtgtt ggccagcaac ctcgacggtg ccaatcccga
    13441 gcggctggtc ggcgaggtcg tcatggacca cggcttcccg gaacttcggg acggataccg
    13501 aaagctgttg ggcgtctagg gattagcgcc gtgaaacaca gcctcgacgt tgttgccgtc
    13561 ggggtcgcgg acgaacgcgc cgaagtatcc ggggtggtac tcgggccaca gccggggcgc
    13621 gtgcagcgat tcggcgccga gttcgatcgc ggcgtcgtag aacgcgcgca cggcgccctc
    13681 gtcggccgcc tggaacgcga cgtggatctc gcggttgggg ccgaggtccg cgtcggcgat
    13741 ccagaagtcg ggcttaccgt ccttgccgta gccgatcgcg acgccgaagt ccatctgccg
    13801 tgtgaagccc agcacgccga gcacccggtc gtagaactcc ttggccttac cgaaatcgct
    13861 gcagttgatg cccaagtggt ctatcacggc gcccatcctg acatttacag tcgattcatg
    13921 agctacgacc ttgtcatccg cggcggcaca atcgtcgacg gtctgggcgg cgaaccgttc
    13981 gtcggtgatg tggctgtgtc agacggggtg atcgtcgcgg tcgggactgt cgacggttcg
    14041 gcggcccgcg agatcgacgc gtcgggtctt ctggtgacgc ctggcttcgt cgacttgcat
    14101 acgcactacg acgggcaggc catctggtcg gaccgactga ccccgtcgtc gcgtcacggc
    14161 gtcaccacgg cggtgatggg caactgcggc gtcggctttg cgccgtgtcg cgccgacgac
    14221 cacgacgtgc tcgtcgacgt gatggccggc gtcgaggaca tccccggcgt cgtcatggca
    14281 gacggcctgc cgtgggactg ggagacgttc cccgagtact tgaatgcgtt ggatgcgcgg
    14341 caccgcgaca tcgacgtggc cgcatatctg ccgcactcgc cgctgcgggt gtacgtgatg
    14401 gggcagcgcg gagtcgaccg ggagccggcg acggccgacg atctggcacg gatgcgcgcg
    14461 ttggccaagg aggcgatcga agtcggcgcc gtcggcttcg cgtcctcacg cttcgtgatg
    14521 cacaaaacgg ctgggggcca acagatcccg acctacaatg ccgcttgcga agagatctct
    14581 gcgattgccc agggcgtcgc cgacggcggc ggcgggcttc ttcagttcgt acccgacatc
    14641 ccggcgggcg gctacgaatc ggtgttgggc caggtcttcg aggtggccca ggacgtcggg
    14701 cttccggtga cgttcagcat ggccaccggc aacagcggcg acccggtgtg gccggacgcg
    14761 ttcacgctga tcgagaagtt cacgtcggcg ggggtcgaca ttacggccca gatgttcccg
    14821 cggccgatcg ggttggtcat cggcctcgag ttgagcggca acccgttcat cctctatccg
    14881 agctatcgct cgatagccga cctaccactg gccgagcggg tcgccgagat gcgcaaaccg
    14941 caagtacggg aacggattct ggccgacaag ccgaccgccg agggcaatcc tctgctgtac
    15001 ctggcgcaga tgtggcagtg gatgtatccg ctcggcgata ccccggatta cgagccttcg
    15061 gcgtcgacga gcatttcggc gcgtgccgcc gcacgcggcg tcaccccgca ggaggaggcc
    15121 tacgaccggc tgctcgacga cgacggccac gcgatgctgt tggtggcgat gggcaactac
    15181 caggacaact cactcgacac cgtcggcgag ctgctgcgcc gcgacgacgt cgtgctcggg
    15241 cttggcgacg gcggcgcgca ctacggaatg atctgtgacg caagctttcc cacgttcctg
    15301 ctgacgcact gggcgcgcga ccgggctcac gggcggatga gcgttgcgga ggcggtgcgt
    15361 gagttgacgt cggtgccggc gcgggtggcg ggtctggccg accggggccg aattgccttg
    15421 ggctacaagg ctgatctcaa tgtgatcgac cacgccggcc tgcggctgca ccggccggtg
    15481 atcacgtacg acctgccggc tggcggccgt cggctggacc agaccgccga agggtacgtc
    15541 g
//
LOCUS       DASWBF010000111         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_339201,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000111 DASWBF010000000
VERSION     DASWBF010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339201"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VF874_03865"
     CDS             complement(<1..322)
                     /locus_tag="VF874_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9175255.1"
                     /translation="MTAATVCRTCGTEPLENARFCHSCGAPITGETPAEYKQATVLFA
                     DVVHSMDIAAAVGPERLREIMAELVTRSTAVVQRYSGTVDKFTGDGIMALFGAPIALE
                     DHAVR"
     gene            411..1559
                     /locus_tag="VF874_03870"
     CDS             411..1559
                     /locus_tag="VF874_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9175256.1"
                     /translation="MNGEFTVTQKRALAVFTVIAIVFGAYFLRGYFILVVVAAVGAYL
                     FTPLFTWLTKRLNTGVSATLTLLAALLAVVIPATLFVLLATVQITQMVKSVANWVGAT
                     DLSTLGDKTLRTVNEMLARVPFLHVNVTQERLQKWMTTAAQHVGQWLLQFLETAAGSV
                     IGAVTSAIIFLYVFISLLVNRHGVITLIRRLNPLGEEVTDLYLAKMGAMVRGTVRGQF
                     VIAACQGVAGALSIYIAGFHEGFFIFAILLTALSIIPLGGGIVTIPFGIGMVLFGNVF
                     GGVFVVLWHLIVVTNIDNFLRPILVPREARLDPALMLLAVFSGIAMFGFWGIVIGPVV
                     MIVIVTTISVYLAVYKGVPLEQHDEDDKPKRRWRFRRKPGAVNRPTAP"
     gene            complement(1537..>2316)
                     /locus_tag="VF874_03875"
     CDS             complement(1537..>2316)
                     /locus_tag="VF874_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9175257.1"
                     /translation="LTGWTAAPFTAEGLTHDVYRKGEGPGVVLIPEIPGLTPQVLALG
                     NYLVDNGFSVVSPSVVGEPGRPLSVGYLVSTMARACVAREFMAFATNAERPVARYLRV
                     LARDLNERTPGKGVGVIGMCFTGGFALAAAVDDSVLAPVMSQPSLPFPLTPAQRRDPG
                     LSEKELQRVVQRATDEGLCAMGLRFTGDRAVPSERFATLKQRLGDAFEVIEIDSSPGN
                     EHGFGRMVHSVLTDQVREKDGHPAYEARKRVVEFFKERLVG"
BASE COUNT          388 a          778 c          731 g          419 t
ORIGIN      
        1 cccgaacagc gtggtcctcc aacgcgattg gcgctccgaa cagagccatg atgccatcgc
       61 cggtgaactt gtccaccgtc ccgctgtacc gctgaaccac cgccgtcgaa cgcgtgacca
      121 actccgccat gatctcgcgc aaccgctccg gacccaccgc cgccgcgatg tccatcgagt
      181 gcaccacgtc ggcgaacagc acagtcgcct gcttgtactc ggccggcgtt tcgccggtaa
      241 tcggcgcgcc gcacgagtga cagaaccgtg cattctccag tggctcggtg ccgcacgtcc
      301 ggcacacggt ggctgccgtc acggcgtcag aataggctga tcggccccgt tcaggacgta
      361 tatcgcgagg cggcgagacc acgccgccgc ggctgactaa cgttctcgcg atgaacggtg
      421 aattcacagt tacccagaag cgggcgctgg ccgtcttcac ggtgatagcg atcgtcttcg
      481 gcgcatactt tctgcgcggc tacttcatcc tcgtcgtggt ggccgccgtc ggcgcctacc
      541 tgttcacgcc gttgttcacc tggctcacca agcgcttgaa caccggtgtc tccgcgacgc
      601 tgacactgct ggcagcgctg ttggcggtgg tcatacctgc gacgttgttc gtgcttctgg
      661 cgacggtgca gatcacgcag atggtcaaga gcgtcgccaa ttgggtgggg gccaccgatc
      721 tgagcaccct cggcgacaag acgctgcgga ccgtcaacga gatgctggcg cgggtgccct
      781 ttctccatgt caacgtcact caggagcggc tgcagaaatg gatgacgacg gccgcgcagc
      841 acgtcggtca gtggctgctg cagttcctgg agactgccgc gggaagcgtc atcggcgcgg
      901 tcacgtcggc gatcatcttc ttgtacgtgt tcatatcgct gctggtgaac cgccacgggg
      961 tcatcaccct gatccgacgg ctgaacccgt tgggcgaaga agtcaccgac ttgtatctgg
     1021 ccaagatggg cgcgatggtg cggggaacgg tcaggggaca gttcgtgatc gcggcatgtc
     1081 aaggggtggc gggtgccttg tcgatctaca tcgccggctt ccacgagggc ttcttcatct
     1141 tcgccatcct gctgaccgcg ctgtccatca ttcccctcgg cggcggcatc gtgacgatcc
     1201 cgttcggcat cggaatggtg ttgttcggca acgtgttcgg cggcgtgttc gtcgtactgt
     1261 ggcacctgat cgtggtgacc aacatcgaca acttcctgcg cccgatcctc gttccgcgag
     1321 aggcgcgcct ggatcccgcg ctgatgttgc tcgccgtgtt ctccggcatc gcgatgttcg
     1381 gcttctgggg catcgtgatc ggcccggtcg tgatgatcgt gatcgtgacg acgatcagcg
     1441 tctacctggc cgtctacaaa ggcgtgccgc tggaacagca cgacgaggac gacaagccga
     1501 aaagacggtg gcgcttccgg cgcaagcccg gggccgtcaa ccgaccaacc gctccttgaa
     1561 gaactcgacg acgcgcttgc gcgcctcgta ggccggatga ccgtccttct cccggacctg
     1621 atcggtcagc acggaatgca ccatgcgacc gaacccgtgc tcgttgcccg gtgacgagtc
     1681 gatctcgatg acctcgaacg cgtcgccgag ccgctgcttc agcgtcgcga accgctcgct
     1741 aggcacggct cgatcaccgg tgaaccgcag gcccatcgcg cacaatccct cgtcggtggc
     1801 ccgctggacg acgcgctgca actccttttc ggagagcccg gggtcgcggc gctgcgccgg
     1861 tgtcagcgga aacggcagcg acggctggct catcaccggc gccagcacgc tgtcgtcgac
     1921 ggcagccgcg agcgcgaacc cgccagtgaa gcacatgccg attacgccga cgccttttcc
     1981 gggcgtgcgc tcgttgaggt cgcgggccag cacccgcagg taccgcgcga ccggccgctc
     2041 ggcgtttgtg gcgaacgcca tgaactcgcg cgccacgcaa gcacgcgcca tcgtcgacac
     2101 caggtagccg acgctcagcg gccgaccggg ctcgccgacc acagacggcg acacaaccga
     2161 aaacccgttg tccaccaagt agtttcccag cgccagcact tgcggagtca agccgggtat
     2221 ctcggggatc agtaccacgc ccgggccctc gcccttgcgg tacacgtcgt gggtaaggcc
     2281 ctcggcggtg aacggggccg cggtccagcc ggtgag
//
LOCUS       DASWBF010000112        12673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_406779,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000112 DASWBF010000000
VERSION     DASWBF010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406779"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="VF874_03880"
     CDS             <1..1600
                     /locus_tag="VF874_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9175258.1"
                     /translation="ADVAVDAVDFPFDTGLYVQLTARGPDGVVLACAAVRAANGDYTA
                     ARDLLDERLLAAEPMKGGWVLAVIYFRSKRWHDVRRVLAPLSAQARNDDPLLYQAMSV
                     AVGLAGAYLGLWEQAFEQLSPHGNGPIAAANAEALLAAGLCARALGRAQTATALLNDA
                     YAVVGIQESLRNTIAAALSDPGYGIHPTTAARIDARTDYWDPNTEPGERDFARQLGAA
                     RRDELRAEAAAELADFVGMVDVKEQIARLESSVRAAKQREALGLPVRNKSLHLILKGP
                     PGTGKTTIARVIAKLLCAADVLPSDTFVETSRADLVDRVIGGSEAKVKDIIARIIDSG
                     GGVLFIDEAYALTDSGSDNDFGPLVIAELIRAMVNHADKLMVIAAGYADKMQEFLDSN
                     EGLRSRFTRGITLPSYTVDELVEITVRKAAKGGSLIEDIEPLRAAYTELAAATAADTS
                     GRRRRALDVLGNARFAENLIQFAEEQRDHRLDVSGKLGPETTVEDLQTITGEDLRTAV
                     ARELDRADKEQHIKLSRVAVGGAS"
     gene            1597..3108
                     /gene="eccB"
                     /locus_tag="VF874_03885"
     CDS             1597..3108
                     /gene="eccB"
                     /locus_tag="VF874_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HEX9175259.1"
                     /translation="MTAPIQPRRGFKSKSPDLGPNAARSRPWGFVTKHQISGWRFLIR
                     RISNGVALHDTRMLTDPLRRQGRALSIGALLGAVLLAGAFILSILKPAGASGNHPILA
                     ERSTNALYVVVNDQLHPVLNLASARLIVGKPDNPTVVKSNEIDKFTLGNTLGIPNAPS
                     RMVQHNSRDARWMVCDAVGGPDTGTTVISADPVPGPGHATALPATSAILATSDGGRTT
                     WLICGNKRSQIDLHNPAVTAAVGINVDTPAPRAIDRALLNLIPESPPLVVPFLSNAGD
                     PPRFVWPAPEMAAPPVGAVVVDHDEDNKLRYYAVTAEGIQQISPVIAAILRANDAYGL
                     VEPPALTPDQVAKAAKADPVPVDDYPADRLQVLDPITDPVSCGLWVKLDGAPTSSMAL
                     LAGQSLPVAEDAKPLSLTEAGPTTAQRVILPRGSGYFVQVTGQQPQSATKESVFWVSD
                     LGVRYGLEAAEHEPTGPAEALGMTAAPLPIPWSVLSLFAPGPTLSKADALVAH"
     gene            3122..7282
                     /gene="eccCa"
                     /locus_tag="VF874_03890"
     CDS             3122..7282
                     /gene="eccCa"
                     /locus_tag="VF874_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCa"
                     /protein_id="HEX9175260.1"
                     /translation="MRERFPQRRRLSGPQRKDKDFILEAPPELPRTIEVSAMRKALPW
                     VFGVVIIGMVVMMFVTGFRQMNPMYLFFMAMMAIALFQSMQAQGGNSEMSTPEVNSER
                     AEYLRYLSGKTEEIRAAATAQRASAQWSHPDPDVLEAVLGSPRMWERSASDPDYLQLR
                     VGRDEVKLASKIKVKPVDSELDLEPVAKTALQHMRAVQQSIPHCPKAIDFSGVGMIAV
                     YGDRTLLGATVRAWIAQLVCWHTPNDTALAVVSPHLESRWEWAKWLPHTESHDIDGAG
                     PARFLGASLREVEAMLDPLLKERTKLVDDKGAVDAAAVTKANKHIVLIVDDPDAAPAV
                     VRRIGARDGVTVIAYRVGAGPDRDYAPHPRELLLRVDRSGHAADAAVRMQSWQGFRWQ
                     TFCAEPDLLDAQVSRHLARQMSKWDAAPTGRRDAESAAAQNMLSLLGIANAAKFDVNA
                     LWAPRMLPVGTGEPVDLEPLLQVPIGLQPSGAPLITDLKDEADGGNGPHGLMIGMTGS
                     GKSTALKAMAFGLFARHCPDVVQAILVDFKDGAGFDAFADYPHVVAVITNLEEKRSLV
                     ERFGETLFGLLDARGRVFHETGNQIKGAAFESLREYNEVRATPAGAHLPAMPYLFVWV
                     DEFSLLLKDHPDMADVFDTVTRKGRSQGVFFLFASQTLDEGVIKRIPDNTQYRIGLKV
                     ASESMSRRVIGTGDAYHIPDGKNLKGTGYFVRAPGAEPVKYRGFILPDRYEPPTTINR
                     KVISARPRARLFTAGRVEPDPDTVIEEEVAAESVIEGPPRSLVLTVGPQLAAAYGKRP
                     PQLWSPPLDDPIPLDNVLSQAAAVPARPDSPWWPLGEIDRPRQLSHGLLSYSLDNGNV
                     SILGMRNAEVSMVVQTFILAAAARYSPRDIGFYALSYGGPALAALKDLPHLGALGGQD
                     RKELNLRVFGDLDNVVARRRRLFDQHNIGSLAEYRPKRSAGDSRLDDGYPTDIYVIID
                     GWENFIEDNTSLMHPKNPHVKNVERLIGAGHGMHVLVTAADWIKLGNAVQSQLNARYE
                     LKLANTTQSQVRARVEDKMIRPQDRIPADQPGRGINSGGDVIRFAVGRIDGQPTMEDL
                     DAKVRETVAVIGNRYAGQRAVPAPRLLPQLVDPATLGQDRLAGERFALGLRGRDLQPM
                     VVDFAADPLLGVYGDDRHGKSTLITHLLRSVVARRSGCDQVMVCIFDKSRQLSAETRL
                     LVEGEDYYETDFASMADRVAALSQVLDGRTPPPNLTWEQKRDWTFQGPLIYLFVDDLD
                     AIPAQLQIHDRVTAGGPPPSGHGRMVQVWQPLLRHLANARDIGLRVIMTHRAAGLSGV
                     EISPNTVPGQFATQNANRILLGSRTTTDKVSGVKFEDGLPPGRGFVIAANDDNDGYVQ
                     LAAPTRGM"
     gene            7461..7775
                     /locus_tag="VF874_03895"
     CDS             7461..7775
                     /locus_tag="VF874_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HEX9175261.1"
                     /translation="MHLDVTPEALTAASAQVAALTGRLLGANAAHAIATGAILPPGSD
                     IPSEKVAASLIAKGAAHQVMAGMGNTELAASSLGVGESGVSYLVGEQEGVAIYTASSG
                     LA"
     gene            7803..9155
                     /locus_tag="VF874_03900"
     CDS             7803..9155
                     /locus_tag="VF874_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE domain-containing protein"
                     /protein_id="HEX9175262.1"
                     /translation="MAVVAPPIWHAAPPEVHSTLLNFGGTPAGITAAGASWTQLGAQY
                     IAAIAELEGILTAVQTAYQGPSAEQFVKAHLPMLLWMADVVVKAEIAAAAHAEITAAY
                     GSAVATMPTLAELAENHTVHHVLVWTNFFGQNTIPIGINEADYVRMWNLAADVMGSWD
                     ATGTAAVDSVPETPMSPITLMPGVGEAGNIAATTASFATQMQAQTGGASLNGADLMGN
                     KLLVGKAATSPASFADKTAGPTTTEAQSAANQRDAPTQQGLNPENMAGSFLQQASSIG
                     PSAAQSATSAAQGPSQILTSAPQTLASAPQQLGSMLTQFVGTGGSPDLVGQHSVMPVG
                     FPGTAAIKGFNPAGLTSLAGGVFNTGPSKPLMPSTWGSSPTSAAESLTNSARGITPVA
                     TGLPGSSASGSGAGGAGMMGAGAHNRRGNSRRVTTYADDAVDEDADADSDGGMFAMTR
                     "
     gene            9233..9547
                     /locus_tag="VF874_03905"
     CDS             9233..9547
                     /locus_tag="VF874_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEX9175263.1"
                     /translation="MGLLDANVAQLVTSASSFADQASMMRSTIHGAESSALQAQAFHQ
                     GESSAAFQMGHARFVEAAGKINVLLDIAGQNINEGAATYTAQDQMGAEGYNAAAGAFP
                     SV"
     gene            9594..9881
                     /locus_tag="VF874_03910"
     CDS             9594..9881
                     /locus_tag="VF874_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEX9175264.1"
                     /translation="MGHVLYNYPAMLGTASEMGGYNGALRAVGSGIASEQGALAANWT
                     GDTGMTYQAWQAQWNQALEELIQGYQMMTDAHEQNTTTMLGRDLAEGAKWA"
     gene            complement(9921..11519)
                     /gene="eccE"
                     /locus_tag="VF874_03915"
     CDS             complement(9921..11519)
                     /gene="eccE"
                     /locus_tag="VF874_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccE"
                     /protein_id="HEX9175265.1"
                     /translation="MTALVSLLLLGWAVLLPVGRGHQWWHYAVAVAFVAAFVGSWHGQ
                     HISTVARRWMPMASHNRRQRCYRGRDPHAGHRRAEQSPQPRRPRVDRAGALQAQIVIH
                     LRPHPHGLTLPGDSSDQLPWEFVTAWLNRYGVRADSLTVCSVTRTPPPSGLRSDAAAL
                     LTGRTPQHRDTWLTYTLRAENNVDALTARQTTIGNPGASSEEQQPGPQRAALADTTAR
                     RLTAELRERGWLATVSEADEQLPEFAPPAATVRRETWTGTEYSDGFRAVYAVEPGALP
                     AVLDALPTLGTKATWVTVTVRSRGRQPATIEACVGTLTAARPPRHPLPGLDGFHGLHR
                     RVAPALTATGFDHATVDLPTTEAAPCALAELRWPTAAVGVPIGFNRSRQPVYLGLASP
                     EPVRITVTGTPAFHMGIIARLALSGLSVAVYTADASQWRTLANHGGPQQFSMPPTPPP
                     PGAILVSDGSIEVPAGAITVTLRQPQSAQAPSTTIVITQDDRHANLFHISTARHRMLL
                     STRLPSAPPPQIGKNINVGRVPRR"
     gene            complement(11566..>12673)
                     /gene="mycP"
                     /locus_tag="VF874_03920"
     CDS             complement(11566..>12673)
                     /gene="mycP"
                     /locus_tag="VF874_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VII secretion-associated serine protease
                     mycosin"
                     /protein_id="HEX9175266.1"
                     /translation="VAVIDSGVTPQPRLANLNPGGDYIDPAADGLVDCDGHGTVVAGI
                     IAAQPGADGFSGVAPEAALVSIRQSSAQWSPKIPSGGDPQQVKTAGDVATLARAVRHA
                     ADMPGVRVINISLIDCIPSYKQVDQAALGAALRYAAIDKDIVVVAAAGNTGESNCESN
                     PLTDPNRPDDPRNWAAATTISTPCYWQPYVLSVGSLTPEGQPSNFTMAGPWVGIAAPG
                     EQIVSLGNAPNSGLVNGQPSNKEPLAPINGTSFAAAYVSGAAALVRSAYPDLTARQIV
                     NRLVASAHNAARSPSNLVGAGVIDPVAALTWDIPEGEEVPAQMPVVRVPPPAPPPPDD
                     RLPRLIAFGVGITAIAAALIAVTMIGLRRDRNNG"
BASE COUNT         2277 a         4293 c         4153 g         1950 t
ORIGIN      
        1 ggccgacgtc gccgtcgacg cggtggactt cccgttcgac accggtctgt atgtgcaatt
       61 gacggcccgt ggccccgacg gtgtggtact ggcctgcgcg gcagtgcgcg ccgcaaacgg
      121 tgactacacg gcagcccgtg acctactcga tgaacggctg ctggccgccg agccgatgaa
      181 gggcggctgg gtgctggcgg tcatctattt ccggtcaaag cgctggcacg atgtgcggcg
      241 cgtgctggca ccgctgagcg cgcaggcacg caacgacgac ccgttgctgt accaggcgat
      301 gtcagtggct gtggggctgg ccggggccta tctgggcttg tgggagcagg catttgagca
      361 gctgagcccg cacgggaacg gccccatcgc ggcggccaac gctgaggcac tgctggcggc
      421 cgggctgtgc gctcgggcgt tgggccgtgc gcagaccgcc accgcactgc tcaacgacgc
      481 ctacgccgtc gtcggaatcc aggaaagcct gcgcaacacc atcgctgcgg cgctcagcga
      541 tcccggctac ggcatccacc ccaccacggc ggcgcgcatc gacgcgcgca ccgactactg
      601 ggatcccaac accgagcccg gtgagcgcga cttcgcccgc cagctcgggg cggcccgacg
      661 cgatgagctt agagccgaag cggccgcaga actcgccgac ttcgtcggca tggtcgacgt
      721 caaagagcag atcgcccgac tggaatccag cgtgcgtgcc gccaagcagc gcgaagcgct
      781 ggggctgccg gtgcgcaaca agtcgctgca cctgatactc aaggggccac cgggaaccgg
      841 caagacgacg atcgcgcggg tgatcgccaa gctgctctgc gcggctgatg tgctgccgtc
      901 ggacacgttc gtcgaaacca gccgcgccga cctggtcgac cgggtgatcg gagggtctga
      961 ggcgaaggtc aaagacatca tcgcgcgcat catcgacagc ggcggcgggg tgctgttcat
     1021 cgacgaggcc tacgcgctga ccgactcggg atccgacaac gacttcggtc cgctggtgat
     1081 cgctgaactg atccgcgcca tggtcaacca cgccgacaag ctcatggtga tcgccgccgg
     1141 ctacgccgac aagatgcagg agtttctgga ctccaacgag gggctgcgct cgcgtttcac
     1201 ccgcgggatc acgctgccgt cctacaccgt cgacgaactg gttgagatca ccgtccgcaa
     1261 ggccgctaag ggtggcagcc tcatcgagga catcgagccg ctgcgggcgg cctacaccga
     1321 gctggccgcc gccaccgccg ctgacaccag cggccggcgc cgccgagcgc tcgacgtgct
     1381 gggcaacgcc cgattcgccg aaaacctcat ccagttcgcc gaggagcaac gcgatcaccg
     1441 cctcgacgtg agcggaaaac tcggccccga aaccacagtc gaggacctgc aaaccatcac
     1501 cggcgaggac ctgcgcacgg cggtagccag agaactcgac cgcgccgaca aagaacagca
     1561 catcaagctg tccagggtcg ccgtgggagg tgcatcatga ccgccccgat tcagccccgc
     1621 cgcggattca agtccaaaag cccagatctg ggacccaacg ccgcgcgtag ccggccatgg
     1681 ggatttgtca ccaaacacca gatctctggc tggcgctttt tgatccgtcg catcagcaac
     1741 ggtgtggcac tgcacgacac acggatgctc accgatccgc tgcgccggca aggccgcgca
     1801 ctgagcatcg gcgcgctgtt aggtgcggtg ctgctagccg gcgcgttcat cttgtcaatc
     1861 ctcaaaccgg ccggagccag tggaaaccac ccgatactgg ccgaacggtc caccaacgcg
     1921 ctgtatgtgg tcgtcaacga tcaactgcac ccggtcctga atttggccag cgcgcggctg
     1981 atcgtgggta aaccagacaa ccccaccgtc gtcaagtcca acgagatcga caagttcacg
     2041 ctcggcaaca cactgggcat tcccaatgcg ccctcacgca tggtgcaaca caattcccgc
     2101 gacgcccgat ggatggtgtg cgatgcagtc ggtggccctg acaccggcac caccgtcatc
     2161 tccgcggacc cggtgcccgg cccgggccac gccacggctc tgccggccac ctctgcgatc
     2221 ctggccacca gcgacggcgg tagaaccacc tggctgatct gtggcaacaa gcgcagccag
     2281 atcgacctgc acaatccggc ggtcaccgcc gccgtgggca tcaacgtcga caccccggcg
     2341 ccgcgggcga tcgaccgcgc actgctgaac ctgatccccg aatcgccgcc gctggtggtg
     2401 ccgttcctga gcaacgccgg tgacccgccc cggttcgtgt ggccggcgcc ggagatggcg
     2461 gcgccgccgg tgggagctgt ggtcgtcgac cacgacgagg acaacaagct gcgctactac
     2521 gccgtcacag ctgaaggcat ccaacagatt tcacctgtca tcgccgcgat cttgcgcgcc
     2581 aacgacgcct acggactggt cgaaccgccc gcgctgacac ccgaccaggt cgccaaggcc
     2641 gccaaggccg atccggtccc ggtggacgac tatccggccg atcgactgca ggtcctcgac
     2701 ccgatcaccg atccggtgtc gtgcgggctg tgggtgaaac tcgacggcgc ccccacctcg
     2761 agcatggcgt tgctggcggg tcaaagcctg ccggtcgccg aggacgccaa gccgctgtcg
     2821 ctgactgaag ctggacccac caccgcccag cgggtgatcc ttccccgggg aagcggctac
     2881 ttcgtgcagg tcaccggtca gcaaccgcag tcggccacca aggaatcggt gttctgggtg
     2941 tctgacctcg gggtgcgcta cgggctggaa gctgcagagc atgagcccac cggcccggct
     3001 gaggcgcttg gcatgaccgc ggctccgctg ccaatcccct ggtcggtgct cagccttttc
     3061 gcacccggtc ccaccctctc gaaagccgat gcgctggtag cgcactaacg aaagatcaac
     3121 gatgcgagag cgattccccc agcggcggcg tctatccgga ccgcagcgca aagacaagga
     3181 cttcatcctc gaggccccgc cggaactgcc gcgcaccatc gaagttagcg ccatgcgcaa
     3241 agcgctgccc tgggtctttg gtgtggtgat catcgggatg gtggtcatga tgtttgtgac
     3301 cggcttccgt cagatgaacc cgatgtatct gttcttcatg gccatgatgg cgatcgcgct
     3361 gttccaatcg atgcaggccc agggcgggaa ttcggagatg tccacgccgg aggtcaattc
     3421 cgaacgcgcc gaatatcttc ggtacctgtc tggcaagacc gaggagatac gcgccgccgc
     3481 gaccgcccag cgggcaagtg cgcagtggtc acaccccgat cccgacgtgc tcgaggccgt
     3541 cctgggctca ccgcggatgt gggaacgcag cgccagcgac cccgactatc tgcaactccg
     3601 cgtcggccgc gacgaggtga agctggccag caagatcaag gtcaagcccg tcgattcgga
     3661 actggacctc gaaccggtcg ccaagacagc actgcagcac atgagggccg tacagcagtc
     3721 aattccgcac tgtcctaaag cgatcgactt ctccggcgtc ggcatgatcg ctgtctacgg
     3781 ggatcgcacg ctgttgggtg cgacggtgcg ggcgtggatc gcccagctgg tgtgctggca
     3841 cacccccaac gacacggcgc tggctgtggt ctcaccgcat ctggagtcgc ggtgggagtg
     3901 ggcaaagtgg ctgccgcaca ccgaaagcca cgacatcgac ggcgccggtc cggcgcgatt
     3961 tctgggcgca tcgctgcgtg aggtagaagc gatgctggat ccgctgctca aagagcgcac
     4021 caaactcgtc gacgacaagg gtgcggtaga cgccgcggcg gtgaccaaag ccaacaagca
     4081 tatcgtgctc atcgtcgacg atcccgacgc tgcgccggcg gtggttcgtc ggatcggcgc
     4141 ccgcgacggt gtcactgtca tcgcctaccg cgtcggcgcc ggcccagacc gcgactacgc
     4201 tccccacccg cgtgaactat tgctgcgggt ggaccgcagc ggccacgccg ccgacgctgc
     4261 agtgcgcatg cagagctggc aaggttttcg gtggcagacg ttttgcgccg agcctgatct
     4321 gctggatgcg caggtatcgc ggcatttggc ccgacagatg tccaaatggg atgccgcgcc
     4381 caccggccgt cgcgacgccg aatcagcggc cgcgcaaaac atgttgtcgc tgctgggcat
     4441 cgccaatgcc gccaaattcg atgtcaacgc attgtgggcg ccgcggatgc tgcccgttgg
     4501 tacgggtgag ccggtggatc tggagccgct gctgcaggtt ccgatcgggt tgcagcccag
     4561 cggcgcccct ttgatcaccg acctcaaaga cgaggccgat ggtggtaacg ggccgcacgg
     4621 gttgatgatc ggcatgaccg gctcgggcaa gtccaccgcg ctgaaggcca tggcgttcgg
     4681 tttgttcgcc cgccactgtc ctgacgtggt gcaggccatc ctggtcgact tcaaagacgg
     4741 cgccggattc gacgcattcg ccgattaccc ccacgtcgtg gcggtcatca ccaacttgga
     4801 ggaaaagcgt tccttggttg agcgtttcgg tgaaactctg ttcgggctgc tcgatgcccg
     4861 cggccgggtc tttcatgaga caggcaacca gatcaaaggt gcagcgtttg aatcgctgcg
     4921 ggagtacaac gaggtgcgcg ccaccccggc cggcgcccat ttgcccgcga tgccctacct
     4981 gttcgtgtgg gtcgacgaat tctccctgct gctcaaagat caccccgaca tggccgacgt
     5041 gttcgacacc gtcacgcgta aaggacgctc ccaaggggtt ttcttcctgt tcgccagcca
     5101 gaccctcgac gagggggtga ttaagcggat cccagataac acccagtacc gcatcgggct
     5161 gaaggtggca tcggagtcga tgtcgcggcg ggtgatcggc accggcgacg cctaccacat
     5221 tcccgacggc aaaaacctca agggcacagg ctatttcgtg cgggcacccg gtgccgagcc
     5281 ggtcaaatac cgagggttca tcctgccgga tcgctatgag ccgcccacca cgatcaaccg
     5341 caaggtgatc agcgcccggc cccgtgcccg cctgttcacc gccggccgtg tcgagcccga
     5401 tccggacacg gtgatcgaag aggaggtcgc ggctgaatcg gtgatcgagg ggccgccgcg
     5461 gtcactggtg ctcaccgtcg gcccgcagct ggccgctgcc tacggcaagc ggccaccgca
     5521 gttgtggtcg ccgccgctgg acgatccgat cccgctggac aatgtgctca gccaggctgc
     5581 cgcggtcccg gcgcgccccg acagcccctg gtggccgctc ggggagatcg accggccccg
     5641 ccagctcagt cacggcctgc tcagctacag cctcgacaac ggcaacgtgt cgatcctggg
     5701 catgcgcaac gccgaggtgt cgatggtggt gcagacgttc atcttggccg cggcagcccg
     5761 gtattcgccg cgcgacatcg ggttttatgc gctgagctac ggcggtccgg cgttggctgc
     5821 gctcaaggat ctcccccatc tgggcgcctt gggcggccag gaccgcaaag aactcaatct
     5881 gcgcgtcttc ggtgacctcg acaacgtggt ggcccgccgc cggcggctgt tcgaccagca
     5941 caacatcggg tcgctggccg aataccggcc aaaacgcagc gcgggtgatt cgcgcctcga
     6001 cgacggctac ccgaccgaca tctacgtgat catcgatggc tgggagaact tcattgagga
     6061 caacacctcc ctgatgcatc ccaagaatcc ccacgtcaaa aacgtcgaac ggctcatcgg
     6121 tgccggccac gggatgcacg tgctggtcac tgccgccgac tggatcaagc tcggcaacgc
     6181 cgtgcagagc caactcaacg cgcgctacga actcaagctg gccaacacca cccagtcgca
     6241 ggtccgcgcc cgggtcgaag acaagatgat ccggccgcaa gatcgcatcc cggccgatca
     6301 gcccgggcga ggcatcaaca gtggcggcga cgtgatccgc ttcgctgtgg gacgcatcga
     6361 cggccaaccc accatggagg atctcgacgc caaggtgcgc gagaccgtgg ccgtaatcgg
     6421 taaccgctac gccgggcaac gcgcggtgcc cgcgccgcgg ctgctgccgc agctggttga
     6481 ccccgccaca ctgggccaag accggctcgc cggggaacgt ttcgcgctgg gcctgcgggg
     6541 acgcgatctg cagcccatgg tggtcgactt cgcagccgat cccctgctgg gggtctacgg
     6601 cgatgaccgg cacggcaaat ccaccctgat cacccacctg ctgcgcagcg tggtggctcg
     6661 tcgcagcggt tgcgaccagg tgatggtgtg catcttcgac aagagccgcc aactcagtgc
     6721 ggaaacccgg ttgctggtcg agggtgagga ctattacgaa accgacttcg cctcgatggc
     6781 cgaccgcgtg gccgcgctgt cgcaggtcct cgacggcaga accccgccgc ccaacctgac
     6841 ctgggagcag aaacgggact ggacattcca gggtccgctg atctatctgt tcgtcgacga
     6901 cctggacgcc atccccgcgc agctgcagat ccacgaccga gtcaccgccg gcgggccgcc
     6961 gcctagcggc cacggccgga tggtgcaggt gtggcagccg ctgctgcgtc accttgccaa
     7021 cgcccgtgac atcggcctgc gggtgatcat gacgcaccgt gccgcgggcc tgagcggtgt
     7081 cgagatctcg cccaacacgg tgcccggcca gttcgccacc cagaacgcca accgaatact
     7141 gctgggttca cgcaccacca ccgacaaggt cagcggggtg aagttcgagg acgggctgcc
     7201 gcctggccgc ggcttcgtca ttgccgccaa cgacgacaac gatggctacg tgcagttggc
     7261 tgcccctacc cgcggtatgt aaccgcgatg ttaaatgtgc accaccgcag gtgtgtcgcg
     7321 ctcaatgagc atgctgcaat aactgccacg tgtcacgtgc tggcgatacc atcttcccaa
     7381 ccagccagca aacggtatca acaccgccgg gcgtgccggg cgacattcga gcagccaaga
     7441 aaacgagggg acggcatctc atgcaccttg acgtcacacc tgaagcactg acggcagcat
     7501 ccgcgcaggt ggccgcgctg accgggcgcc ttctgggcgc caacgccgcg cacgcgatcg
     7561 ccaccggagc catcctgccc cccggctcag acatcccgtc ggaaaaggtt gcggccagcc
     7621 tgatcgcaaa aggcgcagcc caccaggtca tggcgggtat gggtaacacc gagctggccg
     7681 cctcgtcact gggggtgggc gaatccggtg tcagctacct ggtcggcgag caagaaggcg
     7741 tcgccatcta caccgcctcc tcagggctgg cctaaggccc agctgacgaa gacacagagt
     7801 ccatggctgt tgtagcacca ccgatttggc acgcggcgcc gccggaggtg cactccacgc
     7861 tgctcaactt cggtggcacc cccgccggaa tcaccgccgc gggggcatcg tggacgcagc
     7921 tgggcgcgca gtacatcgcc gcgatcgccg agctcgaagg catcttgact gcggtgcaaa
     7981 ccgcctatca aggcccctcg gctgaacagt tcgtcaaagc ccacctgccg atgctgctgt
     8041 ggatggctga tgtggtggtc aaggccgaaa ttgcggccgc agctcacgca gagatcaccg
     8101 cggcctatgg cagtgcggtc gccaccatgc ccacgctggc cgagcttgcc gaaaaccata
     8161 cggtgcacca cgttctggtt tggacgaact tctttgggca gaacacgatt ccgatcggca
     8221 tcaacgaagc cgattacgtg cgcatgtgga atctcgccgc cgacgtgatg ggcagctggg
     8281 acgccacggg aaccgcggcc gtcgattccg taccggaaac tccgatgtcg ccgatcaccc
     8341 tgatgcccgg cgtcggcgag gccggcaaca tcgccgccac cacagcgtcg tttgccaccc
     8401 agatgcaagc ccagaccggc ggcgcctccc tcaacggcgc cgacctgatg ggcaacaagc
     8461 tgctcgtcgg caaggccgcg accagcccgg catcattcgc cgacaaaacc gccggcccca
     8521 ccaccacaga ggcgcaaagc gccgccaatc aacgcgacgc ccccacccaa cagggactca
     8581 acccagaaaa catggccggc agcttccttc agcaggcgtc gtcgatcggc ccgagcgcgg
     8641 cccaatcggc tacttcagcc gcgcagggcc catcccagat cttgacctcc gccccgcaaa
     8701 cactggcctc tgcgccccag caactgggca gcatgctgac ccagttcgtc ggtaccggcg
     8761 gcagcccaga cctcgtcggg cagcacagtg tcatgcccgt cgggttcccg ggcaccgcag
     8821 cgatcaaggg attcaacccc gccggactga ccagcctggc cggcggcgtg ttcaacaccg
     8881 gaccctccaa gccgttgatg ccctcaacat gggggtcgtc gcccaccagt gcggcagagt
     8941 cgctgaccaa cagcgcccgc gggattacgc cggtagcaac cgggctccca ggctcgtcgg
     9001 ccagcggcag cggcgctggc ggcgccggaa tgatgggcgc tggcgcgcac aacagacggg
     9061 gcaactcgcg gcgagtcacc acctacgccg atgacgctgt cgacgaagac gccgacgccg
     9121 acagtgatgg ggggatgttc gcgatgaccc gctgacacac aacctttccc tccgcggatc
     9181 gacacaactc aacacagaca tcagatatcc cacgtcagga aaggacatca ccatgggatt
     9241 gcttgacgcg aacgttgcac agctggtcac ctcggcttcc agtttcgccg accaggcatc
     9301 gatgatgcgc agcaccatcc acggtgcgga aagctcggcg ctgcaggcgc aggccttcca
     9361 ccaaggcgag tcgtcggcgg cgtttcagat gggccatgcc cgcttcgtcg aggcggcggg
     9421 caagatcaac gtactgctcg acatcgccgg ccaaaacatc aatgagggcg cggccaccta
     9481 caccgctcag gatcagatgg gcgccgaggg ctacaacgcg gcagccggcg cgttccccag
     9541 cgtgtaatcc accaaccaga ccaatagaca cgaagcgaaa ggacatctga ctcatgggcc
     9601 acgttctgta caactacccg gcgatgcttg gcaccgccag cgagatgggc ggctacaacg
     9661 gcgcactgcg ggccgtcggt tcgggcattg ccagcgagca gggcgccctg gccgccaact
     9721 ggaccggcga caccggcatg acctaccagg cctggcaggc ccagtggaac caggcgctcg
     9781 aggaactcat ccagggctac cagatgatga ccgacgcgca cgagcagaac accaccacca
     9841 tgctcggccg cgacctggcc gagggcgcca agtgggcgtg agcaccgcag cgctgtagct
     9901 cgacgccaga tgtggggcgg tcaccgacga ggtacccgcc ccacattgat gttcttgccg
     9961 atttgcggcg gtggggcact gggcagacgg gtgctgagca gcatccggtg gcgcgcggtg
    10021 ctgatgtgga acagattggc gtgtcggtcg tcttgggtga tgacgatcgt cgtcgatggt
    10081 gcctgcgcgg actgcggctg gcgcagcgtc accgtgatcg caccggcagg cacctcgatg
    10141 ctgccgtcac tgacgaggat ggcccctggc ggcggcggtg tcggcggcat cgaaaactgt
    10201 tgcgggccgc catgattggc cagagtccgc cactgcgaag cgtcggcggt gtacaccgcg
    10261 accgacaagc ctgatagcgc caggcgcgcg atgatgccca tgtggaatgc cggggtgccg
    10321 gtcacggtga tacgcaccgg ctccggcgac gccaggccca aatacaccgg ctggcgggag
    10381 cggttgaacc cgatcgggac accaacagca gccgtaggcc agcgcagctc ggcgagagcg
    10441 cagggcgccg cctcggtagt gggcagatcc accgtggcgt gatcgaatcc ggttgcggtc
    10501 aacgccggcg cgacccgacg gtgcaaccca tgaaaaccgt ctagtcccgg cagcgggtgc
    10561 cgcggcggcc tggcggcggt cagcgtgccc acgcacgcct cgatcgtggc gggctggcgg
    10621 ccccgtgagc gcacagtgac cgtcacccac gtggctttgg tgcccagcgt cggcaacgcg
    10681 tccaaaacgg cgggcagggc gccgggctcg acggcgtaga cggcgcggaa gccatcggaa
    10741 tactctgtgc cagtccacgt ctcacgacga acggtggccg ctggcggcgc aaactcgggc
    10801 agctgttcgt cagcctcgct cacggtggcc agccatccgc gctcccgtaa ctcggcggtc
    10861 agccggcgcg cggtggtgtc ggccagcgcc gcccgctgcg gccctggctg ctgctcctcg
    10921 gaagaggcac caggattacc tatcgtggtt tggcgcgcgg tcagtgcgtc cacgttgttc
    10981 tcggcgcgca gcgtgtaggt cagccaggtg tcgcggtgct gtggggtgcg gccggtcagc
    11041 agcgcggccg cgtcgctgcg cagtccgctg ggcggtggcg tgcgcgtcac cgagcagacg
    11101 gtgagcgagt cagcgcgcac cccgtagcgg ttcagccacg ccgtgacgaa ctcccacggc
    11161 agttggtcgc tggagtcgcc ggggagggtg agcccatggg ggtgcgggcg gagatggatg
    11221 acgatctgcg cctgcagtgc accggcccgg tcgacccgtg gccggcgcgg ctgcggtgac
    11281 tgctcggcgc gccggtgacc cgcgtgtggg tcgcggccac ggtagcagcg ctgacgccgg
    11341 ttgtgcgatg ccatcggcat ccagcgccgc gccaccgtcg aaatgtgctg gccgtgccag
    11401 gaacccacga aggccgcgac gaaggctacc gccactgcat agtgccacca ctgatgccct
    11461 cggcccacgg gcagcagcac cgcccagccc agcagtagca gcgagaccag cgcagtcaac
    11521 cgccgccagc tcggccgcgc gggggtgaaa cgggatgggc ggcggctagc cgttgttgcg
    11581 gtcacggcgc agtccaatca tggtcacggc gatgagggcg gcggcgatcg cggtgatgcc
    11641 gacaccgaag gcgatcagac ggggcagacg gtcatccggt ggcggcggcg ctggcggcgg
    11701 gacacgcacc accggcatct gggccggcac ctcctcaccc tcgggaatgt cccaggtcag
    11761 cgccgctaca gggtcgatca ccccggcgcc caccagattc gagggcgatc gcgcagcatt
    11821 gtgcgccgag gcgaccaacc ggttgacgat ttgtcgggcg gtcaggtcgg ggtaggccga
    11881 gcgcaccagg gccgcggccc cggagacgta ggccgcagcg aagctggttc cattgatcgg
    11941 cgccagcggc tctttgttgg acggctggcc gttgaccaga ccggagttgg gggcgttgcc
    12001 cagcgagacg atctgctcgc cgggggcggc gatccccacc caggggccgg ccatggtgaa
    12061 gtttgagggc tggccttcgg gagtcagcga ccccaccgac aacacgtagg gctgccaata
    12121 gcacggagtg gagatcgtcg tcgctgccgc ccagttccgg ggatcatcgg gccggttggg
    12181 gtcggtcaac ggattgcttt cacagttgct ctcgccggtg ttgccggccg cggccaccac
    12241 cacgatgtcc ttgtcgatcg ccgcgtagcg cagcgcagcg cccagggccg cctgatcgac
    12301 ctgtttgtag gacgggatgc agtcgatcag cgagatgttg atgacccgga ccccgggcat
    12361 gtcggcggca tggcggaccg cacgcgccag cgtggcgacg tcgccggcgg tttttacctg
    12421 ctgtgggtct ccgcccgagg ggatcttggg cgaccattgc gccgagctct gccggatcga
    12481 gaccagcgcc gcctcggggg cgacaccgga gaaaccatca gcgccgggct gggcggcgat
    12541 gatgccggcg accaccgtcc cgtgtccgtc gcagtcgacc aggccgtcgg ctgccgggtc
    12601 gatgtagtcg cctccggggt tgaggttcgc cagccgcggc tgaggtgtca ccccggagtc
    12661 gattacggcg acc
//
LOCUS       DASWBF010000113         6678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1257302,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000113 DASWBF010000000
VERSION     DASWBF010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257302"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..969)
                     /locus_tag="VF874_03925"
     CDS             complement(46..969)
                     /locus_tag="VF874_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9175267.1"
                     /translation="MHAFPVPGACIEHSRWAESAGFDGILLADSQNLVGDPFVELGVL
                     SRATTRLGLGTGVINPVTRHPAVVAAAAATVQRESGGRAVLGVGRGDSALVQLGLPRA
                     TTAVLAQFIRDVRRYLHGEIDWIAKESVPPPPIELAASGPRTIGLAATLADRVMLTVG
                     ADPERIAGAIETARRAREDAGLDAHGLRVGAYLNVACDDDLATACQLVRGSAAIFAHF
                     SSTFGHSYDESSHGLSSAEQARSLSVPFLQRFAVVGPAGSCASRLTELIDLGLDRVVV
                     VPGSRDSDPQLLARTNTAFAELVLPRLRQVD"
     gene            complement(1001..1399)
                     /locus_tag="VF874_03930"
     CDS             complement(1001..1399)
                     /locus_tag="VF874_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9175268.1"
                     /translation="MNQAHIAGQRSREAAMAHDKAAWLDLFADDAIVEDPIGPSHFDP
                     EGKGHRGREAISAFWDKAIAPNKLEFNFVDTFQCGNEEANVGHIVITTGGYQVTAEGV
                     FTYKANDEGKIVALRAYWEMDRAAKSARKV"
     gene            complement(1396..2277)
                     /locus_tag="VF874_03935"
     CDS             complement(1396..2277)
                     /locus_tag="VF874_03935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9175269.1"
                     /translation="MKYTCSVAMGPIDQLIALAKTAEEVGFDSIALPDSLFYMEKQSA
                     DYPYTPDGSRMWNENTPWVDPLIAAGAMGAVTSTLRFYTNVMKLGSRNPLLLARQVGS
                     VANLTNNRFGFGVGIGWAPEEFEWCGVPYKRRGARVDEMIEVIKLVLGGGMVEFHGEF
                     YDFGRLQMSPAPSEPVPFYVGGHTDVALKRAARVGDGWTSAMMKADQLAETIRALNKY
                     RAEYGRSDEPFEFQAVCIDKFGVDGHRELAEIGVTDNIVIPWIFDGLSFDAPLKDKQD
                     SLKRFADTYIHSGWQNK"
     gene            complement(2304..3488)
                     /locus_tag="VF874_03940"
     CDS             complement(2304..3488)
                     /locus_tag="VF874_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX9175270.1"
                     /translation="MAGQLAAVLGTGQTKYVAKRHDVSMNGLVREAIDRALEDSGSTF
                     DDIDAVVVGKAPDFFEGVMMPELFMADAVGATGKPLIRVHTAGSVGGSTGVVAASLVQ
                     SGKYRRVLAMAWEKQSESNAMWALSIPVPFTKAVGAGAGGYFAPHVRAYIRRSGAPTH
                     IGAMVALKDRLNGSKNPLAHLQQPEITLDKVLSSQMLWDPIRFDETCPSSDGACAVVI
                     GNEEIADARVADGHPVAWIHATALRTEPLAFSGRDQVNPQAGRDAAAALWKAAGITSP
                     IDEIDVAEIYVPFSWFEPMWLENLGFAPEGEGWKLTEAGETAIGGRLPVNPSGGVLSS
                     NPIGASGLIRFAESAIQVMGKAGEHQVPGARKALGHAYGGGSQYYSMWVVSSDKPADK
                     PR"
     gene            complement(3488..4552)
                     /locus_tag="VF874_03945"
     CDS             complement(3488..4552)
                     /locus_tag="VF874_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX9175271.1"
                     /translation="MTERDIAVVGFAHAPHVRRTDGTTNGVEMLMPCYAQVYDQLGIT
                     KTDIGFWCSGSSDYLAGRAFSFLSAIDSIGAVPPINESHVEMDAAWALYEAYIKLLTG
                     EVDTALVYGFGKSSAGVLRRVLALQTDPYTVAPLWPDSVSIAGLQARLGLDAGKWSFE
                     AMAQVALDSFTAAERVDNEKPAKSIDELLARPFFADPLRRHDIAPITDGASAIVLAAG
                     DHARELRENPAWITGFEHRIETPVLGARDLTTSPSTAASAQAATGGDVGAIDVAEIYA
                     PFTHQHLILTEAIGLPASTKVNPSGGALAANAMFSAGLERIGFAAQHIWDGSAQRVLA
                     HATSGPALQQNLVAVMEGKG"
     gene            complement(4596..5588)
                     /locus_tag="VF874_03950"
     CDS             complement(4596..5588)
                     /locus_tag="VF874_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX9175272.1"
                     /translation="MTVSQSSPASIDTSQPPLSAPLKLSFDYTRSVGPVFSQFFTALR
                     DRRIVGVRGTDGRVHVPPAEYDPVTYEPLREIVPVESVGAVVSWTWQPEPLEGQPLQR
                     PFAWALIKLDGADTALLHAVDAETPEAIRTGARVHAHWVDEPVGAITDIAYFALGGDA
                     EPVSEVTDDREPVKILVVPEAIEIQHSASHEESAYLRALAEGKIIGARTGKEGKVYFP
                     PHGADPATGKPTTEFVELPDKGTVTTFAIINIPFTGQRIKPPYVAAYVLLDGADIPFL
                     HLVTEIDASDVRMGMRVEAVWKPREEWGLGIDNIDHFRPTGEPDADYDTYKHHL"
     gene            5645..>6678
                     /locus_tag="VF874_03955"
     CDS             5645..>6678
                     /locus_tag="VF874_03955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9175273.1"
                     /translation="MKLGLQLGYWGAQPPANAPELVGAAEEAGFDTVFTAEAWGSDAF
                     TPLAWWGSSTRRMRLGTSVVQLSARTPTACAMASLTLDHLSGGRHILGLGVSGPQVVE
                     GWYGQKFPKPLARTREYIDIVRQVWAREAPVTSEGPHYPLPLSGEGTTGLGKALKSIT
                     HPLRADIPIMLGAEGPKNIALAAEICDGWLPIFYTPRMADTYNEWLDEGFARPGARRS
                     REDFEICATATIVITDDRSATFELMKPFLALYMGGMGAEDTNFHADVYRRMGYADVVD
                     DVTKLFRSQRKDEAAEIIPDELVDDAAIVGDTDYVRKQIAVWEAAGVTTMVVTPRSAD
                     EITHLGELVL"
BASE COUNT         1127 a         2239 c         2143 g         1169 t
ORIGIN      
        1 gtatctacct gtcgctccat caaccgccga cgcgggggca aagggttagt cgacctgtcg
       61 caggcgggga agaaccaact ctgcgaacgc ggtgttggta cgggcaagca gttgcggatc
      121 cgaatcgcga gatcctggga cgacgacgac gcggtcgagc cccaagtcga tgagctcggt
      181 taagcggctg gcacatgacc ctgcagggcc gacgacggcg aagcgttgca ggaaggggac
      241 cgataacgac cgtgcctgtt cggcgctgga gaggccgtgg ctcgattcgt cgtatgagtg
      301 tccgaatgtg ctcgagaaat gggcgaagat cgcagcgctg ccgcgcacca gttggcacgc
      361 ggtcgcgagg tcgtcatcgc atgccacgtt cagataggcg ccgacccgta gcccgtgtgc
      421 gtcgagtccg gcgtcctcgc gtgcccgtcg cgctgtctcg atggcgccgg cgattcgttc
      481 ggggtcggcg ccgaccgtca gcatcacccg gtcagccagg gttgccgcca gcccaatcgt
      541 gcggggcccg ctagcagcca gttcgatcgg cggtggaggc accgattctt ttgcgatcca
      601 atcgatctca ccgtgcagat accggcgtac atcgcggatg aactgggcca gtactgccgt
      661 cgtcgcacgt ggcagaccga gctgcaccag cgccgaatca ccgcggccca caccgagtac
      721 cgcgcgtcca ccggattcgc gttgcacagt ggctgccgcc gcagcgacga cggcaggatg
      781 ccgagtcacc ggattgatga caccggtacc caatcccagg cgtgttgtcg cgcggctcag
      841 cacgccgagt tcgacaaagg ggtcgccaac caggttttgg ctgtcagcca acagaatacc
      901 gtcgaatccc gctgattcgg cccaccggga atgctcgatg caggcgcccg gcaccgggaa
      961 cgcgtgcatc cagatctgac acgctgcccg gctcgttctt ctacaccttt cgcgcggatt
     1021 tggcggcgcg gtccatttcc caatatgccc gcagtgcaac aattttgcct tcgtcgttgg
     1081 ctttgtaggt gaacacccct tctgcggtga cttgatatcc acctgtcgta atcacgatgt
     1141 ggccgacgtt ggcttcctcg ttcccgcact ggaacgtgtc gacgaagttg aactcgagct
     1201 tgttgggcgc aatcgccttg tcccagaagg ccgaaatcgc ctcgcggccg cggtggcctt
     1261 tgccctccgg gtcgaaatgc gacggtccga tcggatcctc gacgatggca tcatcggcga
     1321 acagatcgag ccaggccgcc ttgtcatgcg ccatcgccgc ctcgcgcgaa cgctgccctg
     1381 cgatgtgtgc ctggttcatt tgttctgcca tcccgagtga atgtatgtgt cggcgaatcg
     1441 cttcagcgag tcctgcttgt ctttcagcgg ggcgtcgaag ctcagtccgt cgaaaatcca
     1501 agggatgacg atgttgtctg tgacaccgat ttccgcgagc tcccgatgac cgtcgacgcc
     1561 gaacttgtcg atgcacaccg cctggaactc gaacggctcg tcggagcgcc cgtactccgc
     1621 gcggtacttg ttcagcgcgc ggatcgtctc ggccagctgg tcagctttca tcatcgcgct
     1681 ggtccagccg tcgccgacgc gcgccgcgcg cttcaacgcg acgtcggtgt ggcccccgac
     1741 gtagaacggc accggttcgc tgggcgccgg gctcatctgc aaccggccga aatcgtagaa
     1801 ctcaccgtgg aattcgacca tcccgccacc cagcaccagc ttgatcactt cgatcatctc
     1861 gtcgacgcgg gcgccgcggc gcttgtacgg cacgccgcac cactcgaatt cctcaggtgc
     1921 ccagccgatt ccgacgccga acccgaatct gttgttagtc aggttggcca ccgagccgac
     1981 ctggcgggcg agcagcaggg ggttgcgcga accaagcttc atcacgttgg tgtagaagcg
     2041 cagcgtcgac gtgaccgcgc ccattgcgcc cgccgcgatc agcggatcca cccacggggt
     2101 gttctcgttc cacatccgcg acccgtcggg cgtgtacggg taatccgcgg actgcttttc
     2161 catgtagaac agggaatccg ggagcgcaat cgagtcgaag cccacctcct cggcggtctt
     2221 cgcgagggcg atcaactggt cgatcgggcc catcgcgacg ctgcacgtgt acttcatccg
     2281 ttgttggctc ttcgcgcgag cgctcatcgc ggcttgtccg ccggtttgtc ggagctgacg
     2341 acccacatag agtagtactg cgagccaccg ccgtacgcgt ggccgagcgc cttgcgggca
     2401 cccggcacct ggtgctcgcc agccttgccc atcacctgga tcgcgctttc ggcgaagcgg
     2461 atcagcccgg aggcaccgat cgggttcgac gacagcacgc cgccggacgg gttgaccggc
     2521 agtcggccgc cgatcgcggt ttcgcctgct tcggtgagct tccagccttc gccttcgggc
     2581 gcaaaaccca agttctccaa ccacatcggc tcgaaccacg agaacggcac atagatctcg
     2641 gcgacgtcga tctcgtcgat cgggctggtg attcccgccg ccttccacag tgccgcagca
     2701 gcgtcgcgac cggcttgcgg gttcacttgg tcgcggccgg agaacgcgag tggctcggtg
     2761 cgcagcgcgg tggcgtggat ccatgccacc ggatgaccgt cggcaacccg cgcgtcggcg
     2821 atctcctcat tgccgatcac gaccgcgcat gcgccgtctg acgacgggca ggtctcgtcg
     2881 aaacggatgg gatcccagag catctgcgac gacagcacct tgtccagcgt gatttcgggc
     2941 tgctgcagat gcgccagcgg gttcttgctc ccattgagcc tgtccttcaa cgcgaccatc
     3001 gcgccgatgt gcgtcggcgc gccggatcgc cggatgtagg cgcgcacatg cggtgcgaag
     3061 taaccgccag cgccggcgcc gaccgccttg gtgaagggta ccggaatgct caacgcccac
     3121 atggcattcg actccgactg cttctcccac gccatcgcca gcacgcgccg gtacttcccg
     3181 gattgcacca ggctggccgc gaccacgccg gtggacccgc cgaccgagcc ggcagtgtgc
     3241 acccggatca gcggcttgcc cgtggcgccg acggcatcgg ccatgaacaa ctcgggcatc
     3301 atcacaccct cgaagaagtc gggcgccttg ccgacgacca cggcgtcgat gtcgtcgaac
     3361 gtcgaccccg agtcctctag cgccctgtcg atagcctcgc gcactaggcc gttcatcgac
     3421 acgtcatggc gtttggcgac gtacttcgtc tgcccggtgc ccagcaccgc ggccagttgc
     3481 cccgccatca gccttttcct tccatgacgg ccaccagatt ctgttgcagc gcaggtccgc
     3541 tggtcgcgtg ggccagcacc cgctgagcgg atccgtccca aatatgctgc gcggcaaagc
     3601 cgatgcgctc caagcccgcc gagaacatcg cgttggcggc caacgctccg cccgacgggt
     3661 tcaccttcgt cgacgccggc aggccgatcg cctcggtgag gatcaggtgc tggtgggtga
     3721 acggcgcata gatttcggcc acgtcgatcg caccgacgtc accgccggtg gcggcctgcg
     3781 ctgacgcggc ggtcgacggc gacgtggtca ggtctcgggc gccgagaact ggcgtctcga
     3841 tgcggtgctc gaaaccggtt atccaggccg gattttcgcg cagctcacga gcgtgatccc
     3901 cggcagccag cacgatcgcc gacgcgccgt cggtgatcgg ggcgatgtcg tggcggcgca
     3961 gtggatccgc gaagaacggc cgcgccagca gctcgtcgat gctcttggcc ggcttctcgt
     4021 tgtccacccg ctcggccgct gtgaacgagt cgagtgccac ctgcgccatc gcctcgaagc
     4081 tccacttgcc ggcatccagc ccgagccggg cctgcaggcc ggcgatcgac accgagtcag
     4141 gccacagcgg cgcgaccgtg tacgggtcgg tctgcaacgc caacacccgg cgcagcacac
     4201 ccgccgacga cttgccgaac ccgtacacca gcgcggtgtc gacctcaccg gtcaacagct
     4261 tgatgtacgc ctcgtacagc gcccacgcag cgtccatctc gacgtgcgac tcgttgatcg
     4321 gtggcaccgc gccgatcgag tcgatcgccg aaaggaacga aaacgcccgc ccggcaaggt
     4381 aatccgagga ccctgagcac cagaaaccga tgtcggtctt ggtgatgccc agctggtcgt
     4441 acacctgggc gtagcacggc atcagcatct cgacgccgtt ggtggtgccg tcggtccgtc
     4501 gcacatgcgg agcgtgcgcg aagccgacca cggcaatgtc acgttcagtc attgagctgt
     4561 ctcctcttcg cgcaagcgct catcggaagg tccctttaca ggtggtgctt gtacgtgtcg
     4621 tagtcggcgt cgggttcgcc cgtcggccgg aaatggtcga tgttgtcgat gcctagtccc
     4681 cattcctcgc ggggcttcca caccgcctcg acccgcatgc ccatccggac gtcggacgcg
     4741 tcgatctcgg tcaccaggtg taggaacggg atgtcggcgc cgtcgagcag cacgtaggcc
     4801 gcgacatagg ggggtttgat ccgctggccg gtgaacggga tgttgatgat cgcgaacgtc
     4861 gtcaccgtgc ccttgtccgg cagttcgacg aactcggtgg tcggcttgcc ggtggcgggg
     4921 tcggcgccgt ggggcgggaa gtacaccttt ccctctttgc ctgtccgtgc gccgatgatc
     4981 ttgccttccg cgagcgcccg caggtacgcg ctctcctcgt gcgaggcgct gtgctggatc
     5041 tcgattgctt ccgggacgac caaaatcttc acgggctcgc ggtcgtccgt cacctcggaa
     5101 acgggctcgg cgtccccgcc caacgcgaag tatgcgatgt cggtgatcgc accgaccggc
     5161 tcgtcgaccc agtgcgcatg cacccgtgct ccggtcctga tcgcctcggg cgtttccgcg
     5221 tcgaccgcgt gcaacaacgc ggtgtcggcg ccgtcgagtt tgatgagcgc ccacgcgaac
     5281 ggacgctgca gcggctggcc ctccaacggc tcgggttgcc acgtccacga caccacggcg
     5341 ccgacgcttt ccaccggtac gatctcgcgc agcggttcat aggtcacggg gtcgtactcg
     5401 gccggcggca cgtgcacgcg cccgtcggtt ccgcggacac ccacgatgcg acggtcacgc
     5461 agcgcggtga agaactgact gaagacgggc ccgaccgaac gggtgtagtc aaaggacagc
     5521 ttcaacggcg ctgagagggg cggttgggat gtgtcaatcg aggccggact actttggctg
     5581 acggtcacgc catcgagtag aacaggttct aacaagtgtc gcaagaagcg gtcaggaggc
     5641 ggtcatgaag ctcggcctgc aactcgggta ctggggggca cagcccccgg cgaacgctcc
     5701 ggaactggtc ggcgccgccg aagaggcggg cttcgacacg gtgttcaccg ccgaggcgtg
     5761 gggctcagat gcattcacac cgctggcctg gtgggggtcg tcgacgcgac ggatgcggct
     5821 gggcacctcc gtggtgcaac tgtccgcgcg cactccgacc gcgtgcgcga tggcatcgct
     5881 gaccctggac cacctgtccg gaggccgcca catcctcggc ctgggggtgt cgggtccgca
     5941 ggtcgtcgag gggtggtacg gccagaaatt cccgaagccg ctggcacgca cccgggagta
     6001 catcgacatc gtgcggcaag tctgggcccg cgaagcgccg gtgaccagcg aaggcccgca
     6061 ctacccgctg ccgctgagcg gggaagggac caccggcctg ggcaaggcgc tcaagtcgat
     6121 cactcatccg ctgcgggcgg acattccgat catgctgggc gccgagggcc cgaagaacat
     6181 cgcgctggcc gcagagatct gcgacggatg gctgccgatc ttctacaccc cgcggatggc
     6241 cgacacgtac aacgaatggc tcgacgaggg gttcgcacgt ccaggggccc gccgcagccg
     6301 ggaagacttc gagatctgcg caaccgccac catcgtcatc accgacgacc ggtcggccac
     6361 cttcgagttg atgaaaccct tcctcgctct ctatatgggc gggatgggcg ccgaggacac
     6421 taactttcac gccgacgtct accgccggat gggctacgcc gacgttgtcg atgacgtgac
     6481 caagctgttc cgcagccagc gcaaggacga ggccgcggaa atcatccccg acgagctcgt
     6541 cgacgacgcc gcgatcgtcg gcgacaccga ctacgtgcgt aagcagatcg cggtctggga
     6601 agccgcaggg gtgacgacga tggtggtcac cccgcgcagc gccgacgaga taacgcacct
     6661 cggcgagctg gtcctaca
//
LOCUS       DASWBF010000114         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_11986, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000114 DASWBF010000000
VERSION     DASWBF010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11986"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VF874_03960"
     CDS             <1..1404
                     /locus_tag="VF874_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9175274.1"
                     /translation="GGTVFPIDLHVTEVSDAGPRVFTGAIRDITERRRAESALRYHAL
                     HDPLTGLANRVLFRDHLDGALARRARQPGTAAVLFLDLDRFKVVNDDLGHAAGDRLLA
                     QAGDRLRTAVRPADTIARLGGDEFAILLESVDTHADVGSVARRVLDLISDPFPLDTKQ
                     VTVTTSIGIAATDGHQSADELLSQADAALYRAKGSGRNRYEIFDEPLRRWLHGRLNSE
                     IELRKALTLDQIEVHYQPLQALNVGRGIDSVEALARWRHPQRGLLPAAEFIELAEETG
                     VIAALDATVLAMVGRQLQTWGGQFDAPDVAVNLSPRTLASDSLISALTASGIHHPDRL
                     WVEITESVLMTDMAGVAARLDQLRGLGVRIAIDDFGTGHSSLTYLKRLPIDAIKIDKT
                     FVDRLGRDPDDTAIVTAVVTLGHTIGVRVVAEGVETERQRDILTELGCDYAQGHLISR
                     AIPAAGITSLLGPASRA"
     gene            1855..2058
                     /locus_tag="VF874_03965"
     CDS             1855..2058
                     /locus_tag="VF874_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9175275.1"
                     /translation="MAQGTVKWFNAEKGFGFISQDDDSTDVFVHYSAIDSGGYRSLDE
                     GQRVEFNVAQGQKGPQAERVMTL"
     gene            complement(2337..>2543)
                     /locus_tag="VF874_03970"
     CDS             complement(2337..>2543)
                     /locus_tag="VF874_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9175276.1"
                     /translation="RAEKEHVGDPLRRASIQELWAEYRAVAPLNATIHQLRYAHACEL
                     VNVGISLETIRRRLGHANAPTTLR"
BASE COUNT          412 a          902 c          823 g          406 t
ORIGIN      
        1 ggcggcacgg tgttccccat cgacctgcac gtgaccgagg tctccgacgc cgggccacgg
       61 gtcttcaccg gcgccatccg cgacatcacc gaacgccggc gggccgagtc ggcgctgcgg
      121 tatcacgcgc tgcacgaccc actgaccgga ctggcgaacc gggtgctgtt ccgcgaccac
      181 ctcgatggcg cgctggcccg gcgggcccgc cagccgggca cggccgcggt gctgttcttg
      241 gacctggacc gcttcaaagt cgtcaacgac gacctcgggc acgcggccgg cgaccggctg
      301 ctcgcacagg cgggcgaccg gctgcgcacc gccgtccgcc ccgccgacac gatcgcccgg
      361 ctcggcgggg acgagttcgc gatcctcctc gaatccgtcg acacgcacgc ggacgtcggc
      421 agcgtcgccc gccgcgttct ggacctgatc agcgacccgt tccctctcga caccaaacag
      481 gtcacggtca ccacgagcat cggaattgcc gccaccgacg ggcaccagtc cgccgacgaa
      541 ctgctcagcc aggccgacgc cgccctctac cgcgccaagg gcagtggccg taaccgctac
      601 gagatcttcg acgagccgct gcgtcgctgg ctgcacggcc ggctgaacag cgagatcgag
      661 ctgcgcaaag cactgaccct cgaccagatc gaggtgcact accagccgct gcaagccctg
      721 aacgtcgggc gcggcatcga cagcgtggaa gccctggcgc gatggcgaca cccgcaacgc
      781 gggctgctcc cagccgccga gttcatcgag ctcgccgagg aaaccggcgt catcgcggcg
      841 ctggacgcga cggtgctggc catggtcggc cggcagctgc agacctgggg cggccagttc
      901 gacgcgcccg atgtcgcggt caacctgtcc ccgcgcacgc tcgcctccga cagcctcatc
      961 agcgcgttga ccgccagcgg gatccaccac cccgaccgac tgtgggtcga gatcaccgag
     1021 tctgtgttga tgaccgacat ggccggtgtt gctgctcggc tcgaccagct cagagggctc
     1081 ggcgtgcgca tcgccatcga cgacttcggc accggccatt ccagcctgac ttacctcaaa
     1141 cgcctcccga tcgacgccat caagattgac aagacgttcg tcgaccggct cggccgcgac
     1201 cccgacgaca ccgccatcgt caccgccgtc gtcaccctcg gccacaccat cggcgtccgc
     1261 gtcgtcgccg aaggcgtgga aaccgaacgg caacgcgaca tcctcaccga actcggctgc
     1321 gactacgcac aaggccacct catctcccgg gccatccccg ccgccggcat cacctcgctg
     1381 ctcgggccgg ccagccgcgc gtgacggctc gatgttgcgg gaccggtctt cagtgcatca
     1441 gggctaagcc gacccccagc cgccagctga ctcccggccg ggaaacaacc agcgcgattc
     1501 ctcggccggg gtccacgcct tggtcgcacc aagtcctgac gttcacgcga gtcttcatct
     1561 tctttcgagg tttgtcccga ttgctctcgg gctacttctg ctggccttgg cgatcccccc
     1621 cagcggcatc gtcacttcag gtcggcgtgt cctcgggcgg tgcggcggtt gcgttgatca
     1681 ccgcgaatgc gtacactgag cgtaagctgc agtgtcagtt tcagggatct tttgtcaaag
     1741 cccgcgaagt ctgacagcgg gcgtcttctc tttctccatc gtcgggatgg gcagtcgcca
     1801 ggcagcgccg gcgccggaac cgcggcatcg gacaatcccg agagagggag agctatggct
     1861 cagggcaccg tgaaatggtt caacgccgag aagggcttcg gcttcatcag ccaggacgac
     1921 gacagcaccg acgtcttcgt ccactattcg gctatcgact cgggcggcta ccgcagcctc
     1981 gacgaggggc agcgcgtcga gttcaacgtg gcgcagggcc agaaaggccc gcaagccgag
     2041 cgtgtcatga cgctgtaggc ggtctcgcga tccgtacgtc ggcgtgcccg cgagcgcgtc
     2101 ggcgtactga tcggcagccg tctcgcccga tcaggcacca atcggacagc ctccactcga
     2161 ccagcccggc ctcaccgtgg ttaagcgact ccagctacgt aggcaactgc tttcggggtc
     2221 gaggaggatc caacagccgt tggtggtagc gatgatctag gcctggtcga cgctacccac
     2281 gtcggcgcca gatgtgcggg gtctccgcgt cggccgtggt gtcgcgcggg tcggcgttag
     2341 cgcagggtgg tcggagcgtt agcgtggccg agccggcggc ggatggtttc cagggagatg
     2401 ccgacgttga ccagttcgca ggcatgggcg tagcggagct ggtggattgt cgcgttcagg
     2461 ggggccacgg cccggtattc cgcccagagt tcctggatcg aggcgcggcg cagcgggtcg
     2521 ccaacgtgtt ctttctcggc gcg
//
LOCUS       DASWBF010000115         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_734356,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000115 DASWBF010000000
VERSION     DASWBF010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734356"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VF874_03975"
     CDS             complement(<1..286)
                     /locus_tag="VF874_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase"
                     /protein_id="HEX9175277.1"
                     /translation="MTAEFVKRNPAAPQGFFACEAAGLAWLGAVDGGVPCAQVVCYDE
                     TSLTLHRINSVLPTQESARVFGVRLAVTHDAGAAAFGAAPDGWSGPGFFGP"
     gene            complement(283..1107)
                     /locus_tag="VF874_03980"
     CDS             complement(283..1107)
                     /locus_tag="VF874_03980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175278.1"
                     /translation="MPTVLVTGASRGIGKTIATHLADTGWDVIAGVRSEEDGARLSES
                     RITPVTLDVTNENHVAALADSLPERLDAVVNNAGVAVAGPMEAVPLTDFRRQLDVNVV
                     GQIAVTQAVLPRLRSSHRRIVFISSLNGQLSFPLMGAYCASKFALEAAADALRLELKP
                     WGIAVVVVEPAQTDTDMWRTADSVVAETEAAMSPENRELYQRHVAGMKAVVATSQKSA
                     VPPAKVAAVVEEVLTARHPRARYVVGIGPTLQLALLSRMPTGMRDRVLRAVAKQPR"
     gene            complement(1114..1497)
                     /locus_tag="VF874_03985"
     CDS             complement(1114..1497)
                     /locus_tag="VF874_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9175279.1"
                     /translation="MRLWSGDDGQSHVQVGVIAMSRPGEAGPSVLSELVPALWISFEE
                     TPAISSLEWHTAPHRQFVITVGGRLDFVTRDGEQFRLDPQTVLLAEDTVGGGHRWSIV
                     GEDPWRRVYVRLPADAVVPFSPESG"
     gene            complement(1506..1721)
                     /locus_tag="VF874_03990"
     CDS             complement(1506..1721)
                     /locus_tag="VF874_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175280.1"
                     /translation="MTELVRRVDGIEVVQRGISTERIATAVRRSDTELLRRINAAQQE
                     LENDGRLPDFRLQWLGSPVLDQSERRR"
BASE COUNT          296 a          630 c          582 g          283 t
ORIGIN      
        1 gcgggccgaa aaagcccggc ccactccagc cgtcgggcgc ggcaccgaac gccgccgcgc
       61 cggcgtcgtg ggtgaccgca agccgcacgc cgaacacgcg tgccgattcc tgcgtcggca
      121 gcaccgagtt gattcggtgt agcgtcaggc tggtctcgtc gtagcagacg acctgcgcgc
      181 acggcacacc cccgtccact gcgccgagcc acgccaatcc agcggcctcg caggcgaaaa
      241 acccttgcgg cgcagcagga ttgcgcttga caaactccgc ggtcaccggg gctgcttcgc
      301 caccgcccgc agcacgcggt cgcgcatgcc cgtcggcatg cgggacagca gggccagttg
      361 cagcgtgggt ccgatcccga cgacgtagcg ggcgcgggga tgccgggcgg tcagcacttc
      421 ttccacgacg gccgccactt tcgccggcgg caccgcgctc ttctgcgacg tcgcgaccac
      481 ggctttcatc cccgctacgt gccgttggta cagctcgcga ttctccgggg acatcgccgc
      541 ttcggtctcg gcgacgacgc tgtcggccgt gcgccacatg tcggtgtcgg tttgggccgg
      601 ttcgacgacc accacagcga tgccccacgg cttgagctcc aaccgcagcg catccgcggc
      661 cgcctccagc gcgaacttcg acgcgcagta cgcacccatc agcgggaacg aaagctgtcc
      721 gttgaggctg gagatgaaca cgatccgccg gtgggaactt cgcagccgcg gcagcaccgc
      781 ctgcgtcacc gcgatctgcc cgacgacgtt gacgtcgagt tgccgccgaa agtcggtcag
      841 gggcacggcc tccatcgggc cggcaaccgc gacgccggcg ttgttgacta ccgcgtccag
      901 ccgctcgggt agcgaatcag caagtgccgc gacgtgattc tcgttagtca catcgagggt
      961 gacgggagtg atgcgtgatt cgctcaggcg cgcaccgtct tcctcgctgc ggacgccggc
     1021 gatgacgtcc cagccggtgt cggcgagatg ggtggcgatg gtcttgccga tgcctcgaga
     1081 ggcgccggtg acgagaactg ttggcatttc cggttaccca ctttccggac tgaagggcac
     1141 gactgcatcg gcggggagac gaacgtagac gcgccgccag gggtcctcgc cgacgatcga
     1201 ccagcgatgt cccccaccga ccgtgtcctc ggcgagcagg accgtctgcg gatcgagccg
     1261 gaactgctcg ccatcgcggg tgacgaagtc gagccggccg ccgacggtga tgacgaactg
     1321 gcggtgcggc gccgtatgcc actctagtga gctgatcgcc ggtgtctcct cgaagctgat
     1381 ccaaagggcg ggaacaagtt ccgacagcac cgacggccca gcctcgccgg gtcggctcat
     1441 cgcgatcacg ccgacctgca catgcgactg accgtcgtcg ccgctccaca atcgcacaca
     1501 gcggatcatc ggcgccgctc gctctggtcg agaactggcg agcccagcca ctgcaacctg
     1561 aaatcgggta gtcgaccgtc gttttccagc tcctgttgcg cggcgttgat gcgccgcagc
     1621 agttcggtgt cggatcggcg caccgcggtg gcgatccgct cggtcgaaat gccgcgctgc
     1681 accacctcga taccgtcgac gcgacgcacc agctccgtca ataccggcgc aagcgttcat
     1741 gaacacgtca caaccccccg tcgtcaggtc ggtgatcgcg tccctgatgc g
//
LOCUS       DASWBF010000116        14313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_272114,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000116 DASWBF010000000
VERSION     DASWBF010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14313
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272114"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VF874_03995"
     CDS             complement(<1..1022)
                     /locus_tag="VF874_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9175281.1"
                     /translation="MATRASYRDEHLALVAQLRDKLATAALGGSEKARERHVSRGKLL
                     PRDRVDGLLDPGSPFLELSPLAADGMYDDESPGAGIITGIGQVSGRECVIVANDATVK
                     GGTYYPITVKKHLRAQEVARQNKLPCIYLVDSGGAFLPKQDEVFPDREHFGRIFYNQA
                     TMSAEAIPQIAAVLGSCTAGGAYVPAMSDEAVIVRDQGTIFLGGPPLVKAATGEVVTA
                     EELGGGDLHSKTSGVTDHLAHDDRDALRIVRRIVSTLGPRQPYLWDVAPTIDATADQT
                     ELYDVVPIDSRVPYDVHDVVTRIVDGGEFAEFKADYGATLVTGFARIHGHPVGIVANN
                     GVLFSES"
     gene            1088..1714
                     /locus_tag="VF874_04000"
     CDS             1088..1714
                     /locus_tag="VF874_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9175282.1"
                     /translation="MSTTTDAETRRSRLKSDRRSKLLAAAERLIAERGYFAVRLEDIG
                     SAAGVSGPAIYRHFPNKEALLVELLVGISTRLLAGARAVVSEAADDDAALDGLIDFHL
                     EFALGEPDLIRIQDRDLAHLPERAKRQVRKAQRQYVEIWVDSLRQRDGIDETDARLIA
                     HAAFGLMNSTPHGLKAQGANGIGSTRSRSVLRAMTVAALSSGHRGDAR"
     gene            complement(1711..1866)
                     /locus_tag="VF874_04005"
     CDS             complement(1711..1866)
                     /locus_tag="VF874_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HEX9175283.1"
                     /translation="MIVLGIILAVLGWILGISILLYIGVVLIVIGAVLWILGSVGRPV
                     GGRRYWY"
     gene            complement(1863..2129)
                     /locus_tag="VF874_04010"
     CDS             complement(1863..2129)
                     /locus_tag="VF874_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175284.1"
                     /translation="MRGTGIIGVIVVIWLLIGALAAFQRGYFKTGDANCATAARVALT
                     VLAGPLNYFGVNPKVDCEVRVPQPSASGAVFIPQPISSTIGVAS"
     gene            complement(2272..4179)
                     /locus_tag="VF874_04015"
     CDS             complement(2272..4179)
                     /locus_tag="VF874_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9175285.1"
                     /translation="MGDEPLARRADSSVRANYGASLSPDATAFAHIVDDGGYPRAVQR
                     FLRGWRASSSRDVVLPVEGPVERVIHSADGHWLACQVAPEGSERSQIWVVTTDPDDRM
                     ARRIDYEDAGMAELVSWDGPRVAATLTGEDGVGQSCLIDPANGDRIVLDRRSGGRLVD
                     AWGGASLVRVGPRGYQELIMLHGLTEIALLPSDPGSTTDVGVILDDHQPRRIRFGPTG
                     EEMRLYHPAKTYDVNSAEGYVRALIRSDNGCEHARLLEVTATADGVSYFVVAERPDCD
                     LDEFVISDDLSTVALLWNIQGRSELQILEYTDKTLSEAIPLPATVASELSISAGGSMV
                     ALTVEGPSLPRTVELVEPRSREWQRIDREPSRGPVVSGFAVGPTLEATTARDGLRFTA
                     WLYRPPAGAPATGALVYLHGGPEGQARPGYNEIFPHLLNAGITVLTPNVRGSGGFGRA
                     FMHADDKEGRFAAIDDVAECVRFLVDNELADPARIACAGWSYGGYLTLAALTFHPALF
                     AAGISICGMSDLNTFYRNTEPWIAAAAYPKYGHPVGDRELLEELSPLRRVDTLTAPLL
                     VAHGAHDTNVPVGESEQIVEALREAGRPVRFLLFPDDGHGLDKRENRTVLTAAIAEWL
                     TEAFSATRAAS"
     gene            complement(4185..5966)
                     /locus_tag="VF874_04020"
     CDS             complement(4185..5966)
                     /locus_tag="VF874_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HEX9175286.1"
                     /translation="MCGICGEIRFDGRSADVAAVSRMTEAMLSRGPDSDGLVAHGPVA
                     FGHRRLSIIDLSACGGQPMVDSELGLTLVFNGCIYNYKELRAELQAAGYRFFSTADSE
                     VIIKAFHKWGTACVERFKGMFAFAIADRASGVVTLGRDRLGIKPLYIAESPGKLRFAS
                     SVRALLHAGGVDTDLDRVALHHYMSFHAVVPTPWTIYRGVRKLPPATVRVIQPDGRYT
                     DTVYWSPEFRRDPGRKDWSTQDWQNALIDALRVAVSRRMVADVPVGVLLSGGIDSSVV
                     VALLAEQGQHGLATYSIGFDSAGGQSGDEYYYSDMVAKAFDTDHHKIHIDNSRLLPAL
                     PKTIAAMSEPMVSHDCVAFYLLSEEVSKSIKVVQSGQGADEILAGYDWYPPLANIARD
                     QTTDAYAKVFFDRDHSDVVSIFAPEYCVDTDVSREFVTAHQAAPGADTAVDAALRLDT
                     EVMLVDDPVKRVDTMTMAWGLEARVPFLDHDFVELAATCPPDLKLASGGKGVLKDASR
                     SLLPAEIIDRTKGYFPVPGIRHLDGGVLDLVRDALTNDAARARGLYRRDTLETLLNAP
                     NEGRTTLGANSLWQLGVLEMWLQSMEG"
     gene            complement(5972..7192)
                     /locus_tag="VF874_04025"
     CDS             complement(5972..7192)
                     /locus_tag="VF874_04025"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX9175287.1"
                     /translation="MVEADEWAQLCAGLGQRARALDAFLRDVYSDQQIIRDGLLGVHA
                     IDRAPGFRSTGRLAADRVHVHISGTDLVCDRPGHWMVLEDNLRVPSGMGYAVMNRRLL
                     ARHLPELHPPAPATKSDEALQMLCDTLRAAAPPHGGDDAKVALISAGWEDSAWFEHTF
                     LAEEMGVPLVHPSDLSIRDGRVVRHVGTELRRVDVLYVRMDEDMLLSSTGHDGAPLRP
                     GLVRAVADGTLTIANALGNGVADDKAIYAYVPAMIEYYLDEKPKLAQVPTWICAERAQ
                     RDYVLDNLADLVVKPIDGLGGGGVLIGPDASEKELAARRRELLAQPERFIAQELVELS
                     THPTFDGEGMFPHHVDLRVFVHLRGAADGSATAHVAPAALTRVASRGSRIVNSSFGGS
                     SKDTWILMEDDGGG"
     gene            complement(7487..8575)
                     /locus_tag="VF874_04030"
                     /pseudo
     CDS             complement(7487..8575)
                     /locus_tag="VF874_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            8680..9432
                     /locus_tag="VF874_04035"
     CDS             8680..9432
                     /locus_tag="VF874_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HEX9175288.1"
                     /translation="MTDSPRPGQSDPINPYHDPYQAYPDPAYAGSAPLGPSYAPTSMP
                     SNPAPTQPLPQYWTQTYPQTGSMPPVDLPPEPPRGPRPWLWAIAGFSVAVVIGLVVWV
                     VFVNTNPSRQETSIPAMPQSSTSRTPPRTATTTPALPSILPIPLPTLTLPNPTTSPNT
                     SGETEPVLYEVTGEGRAINITYIDNGGVMQTEFNVLLPWQKDVDLPKPAKDSASITVI
                     NAGRNVTCKISIGGVQVQERTGALLTVCSPSG"
     gene            complement(9429..10715)
                     /locus_tag="VF874_04040"
     CDS             complement(9429..10715)
                     /locus_tag="VF874_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9175289.1"
                     /translation="MGSPRSRVLAWALWDCGSTGLNAVVVTFVFNVYLTGTVGADLPG
                     GTSPASWLGRALALAGVVVASLAPVLGVWVDAPQRRRRTLGFLTAGLVLLTSGMSLIR
                     DDPSYLWAGLVLLALAAACSDLASVPYNAMLRQLATPETSGRISGFGWGAGYVGSMVL
                     LLVVYVGFIAGEGPTTGLLNIPTADGQNVRAAMLMTAAWMAAFALPVLIAPPPVHDLD
                     AAIPPKIGLFGAYRKLWSDIVGEWRRDRNVVYYLLASAVFRDGLMGVFTFGGVLAATV
                     YGLSPADVLLFGVVANVVAAVGAIVGGLLDDHIGSKTVILGSLASMIVLGATLMTLSG
                     ATAFWVLGLLLCLFVGPTNSSARTLLLRMTARGKEGVVFGLYTTTGRAVIFLAPLMFS
                     TFVDAFGADRAGMGGLLVVLAAGFVAMLLVHAPKRR"
     gene            10756..11559
                     /gene="cmrA"
                     /locus_tag="VF874_04045"
     CDS             10756..11559
                     /gene="cmrA"
                     /locus_tag="VF874_04045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217025.1"
                     /note="Catalyzes the final step in mycolic acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycolate reductase"
                     /protein_id="HEX9175290.1"
                     /translation="MPVPAPSPDTRAVVTGASQNIGEALATELAARGHHLIVTARRED
                     VLNALAARLTDRYGVTVEVRPVDLVDPAERAKLADELASRNISILCANAGTATFGPVA
                     SLDPADEKAEVQLNVLGVHDLTLAVLPGMVSRGAGGILISGSPAGNSPIPNNATYAAT
                     KAFVNTFSESLRGELKKAGVHVTLLAPGPVRGDLPDDAEASLVDKLVPEFLWISTEHT
                     AKLSLDGLERNKMRVVPGVTSKAMSVASGYTPRAIVAPIVGAFYKKLGG"
     gene            complement(11642..13195)
                     /locus_tag="VF874_04050"
     CDS             complement(11642..13195)
                     /locus_tag="VF874_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX9175291.1"
                     /translation="MSRESSPPPAQQIAAGYAAEGQALELGTVVVDGTVDPTARIRIP
                     LATVNRHGLVAGATGTGKTKSLQVMAEQLSAAGVPVMMADIKGDLSGLSRPGVANDKT
                     AARATDTGDDWVPAAYPVEFLSLGTGGIGVPVRATISSFGPILLSKVLGLNATQESTL
                     GLIFHWADQKGLPLLDTKDLRSVITFLTSDEGKPELKALGAVSSTTAGVILRALVNLE
                     AEGADTFFGEPELDPNALMRVDAQGRGVITLLEVGAQAARPVMFSTFLMWVLADLFTF
                     LPEVGDLDKPKLVFFFDEAHLLFTDASKAFLEQVEQTVKLIRSKGVGVFFCTQLPTDV
                     PNDVLSQLGARVQHALRAFTPDDQKALSKTVRTYPKTNVYDLESALTSLGIGEAVVTV
                     LSEKGAPTPVAWTRMRSPRSLMDTIGPDAIAAAAKTSALQPYFGQTIDRDSAYERLAA
                     KLAPPPEPAQAGGASVSGKMKTPDAAEGGMVEDVLQSSAFKSAMRSAGTVIGREITRS
                     IFGTGRRRR"
     gene            13223..13870
                     /gene="orn"
                     /locus_tag="VF874_04055"
     CDS             13223..13870
                     /gene="orn"
                     /locus_tag="VF874_04055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX9175292.1"
                     /translation="MRDELVWVDCEMTGLDLAKDRLIEIAALVTDGELNVLGDGVDVV
                     IHTDEDALTGMIDVVTEMHTRSGLTDEVRASTVDVAEAEAMVLDYIKAHVPQAKMAPL
                     AGNSIATDRGFIARDMPALDDYLHYRMIDVSSIKELCRRWYPRIYYGQPEKGLAHRAL
                     ADIQESIRELQFYRRTAFVPPPGPSSTEIAAVAAEFNAATSTSGDINSAPEATSG"
     gene            13909..13984
                     /locus_tag="VF874_04060"
     tRNA            13909..13984
                     /locus_tag="VF874_04060"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13942..13944,aa:His,seq:gtg)
BASE COUNT         2462 a         4867 c         4679 g         2305 t
ORIGIN      
        1 gattcgctga acagcacgcc gttgttcgcg acgatgccga ccggatgccc gtggatacgc
       61 gcgaagccgg tgaccagtgt ggcgccgtaa tccgctttga attcggcgaa ttcgccaccg
      121 tcgacgatgc gtgtgacgac gtcgtgcacg tcgtagggaa cgcgcgagtc gatcggcacg
      181 acgtcgtaga gctcggtttg gtcggcggtg gcgtcgatcg tcggcgcgac gtcccacagg
      241 tacggctgcc gtgggcccaa cgtcgacacg atccggcgca cgatccgcag cgcgtcgcgg
      301 tcgtcgtgcg ccaaatggtc ggtgacaccg gaggtcttgg agtgcaggtc gccgcctccg
      361 agttcctcgg cggtgaccac ttccccggtt gcggctttca ccagcggcgg accgccgagg
      421 aagatcgtgc cctggtcgcg gacgatcacc gcctcgtcgc tcatcgccgg cacgtacgcc
      481 ccgccggccg tgcacgagcc cagcaccgcg gcgatctggg gaatggcttc ggcgctcatc
      541 gtcgcctggt tgtagaagat gcggccgaag tgttcgcgat cgggaaacac ctcgtcctgc
      601 ttgggcaaga acgcgccgcc cgagtcgacc aggtagatgc acggcagctt gttctggcgg
      661 gcgacttcct gggcgcgcag gtgcttcttc accgtgatcg ggtagtaggt gccgcccttg
      721 accgtcgcgt cgttggcgac gatcacgcac tcgcggcccg acacttggcc gatgccggtg
      781 atgatgcccg ccccgggcga ctcgtcgtcg tacatgccgt cggcggccag cggcgacagc
      841 tccaggaacg ggcttccggg gtcgagcagg ccgtcgacgc gatcccgcgg cagcagcttg
      901 ccgcgggaga cgtgcctttc tcgggctttc tccgatccgc ccagcgcggc cgtggcgagt
      961 ttgtcgcgca gctgggcgac gagcgcgaga tgctcgtcgc ggtaggaagc ccgagttgcc
     1021 acgtgaggtg ccctgaagtt gagttaatgt cgactaaccg gcgctatgtt agttatgatt
     1081 aaccgaaatg tccaccacca ccgacgccga gacgcggcgc agcaggctca agtccgaccg
     1141 gcgctcgaag ctgcttgccg ccgccgagcg actgatcgcc gagcgggggt atttcgcggt
     1201 gcggttggag gacatcggat cggccgcggg cgtcagcggg ccagcgatct accggcattt
     1261 cccgaacaaa gaagcgctgc tggtcgaact gctcgtcggc atcagcacac gcctgctggc
     1321 cggcgcgcga gcggtcgtgt cagaggcggc cgacgacgat gcggcacttg acgggctcat
     1381 cgactttcat ctcgagttcg ctttgggaga gccggatctc attcggattc aggaccgcga
     1441 cttggcgcat cttccggagc gggccaagcg tcaggtccgt aaagcacagc gtcagtacgt
     1501 cgagatctgg gtcgattcgc ttcgccagcg cgacgggatc gacgaaaccg acgcacggct
     1561 gatagcgcat gcggcattcg ggttgatgaa ttcgacgccg cacggcctga aagctcaggg
     1621 ggccaatggt attgggtcaa cgcgatcgcg ttcggtactg cgggcaatga cggtggccgc
     1681 cctttcgagc ggccaccgtg gggatgcgcg ctagtaccag tagcggcggc cgccgaccgg
     1741 gcggccgacg gagccgagga tccacagcac ggcgccaatg acgatcagca caacgccgat
     1801 gtacagcaga atcgagatgc cgagtatcca accaagaacg gcaaggatga ttcccaacac
     1861 gatcatgacg cgactccaat cgttgaacta ataggttgag ggataaacac cgcgccgctg
     1921 gcactgggct gcggaacccg gacttcacag tcgaccttcg gattgacgcc gaagtagttc
     1981 agtggacccg ccagcacggt gagtgccact ctggcggcag tggcgcagtt cgcgtcaccc
     2041 gtcttgaaat agccgcgctg aaaggccgca agcgctccga tcagcagcca gatcacgacg
     2101 attacaccga tgattccggt acctcgcatt tcgcgcctcc gtccgcacga cttcaggtgc
     2161 aatgcggtta cccaaccgct aaatcagcca aaccacgtcc cgagactaca gccaccgtgg
     2221 tccccactcg cacttatccg cgcggaatgc agtttcggcg atgcggcaca gctaggacgc
     2281 ggcgcgggtt gccgagaacg cctcggtcag ccactcggcg atcgcggcgg tgagcacggt
     2341 gcggttctcc cgcttgtcga gcccgtgccc gtcgtcgggg aacaaaagaa accggaccgg
     2401 ccgaccggct tcgcgtaacg cctccacgat ctgctcggac tcaccgaccg gcacgttggt
     2461 gtcgtgcgca ccgtgagcca ccagcagcgg agcggtcaac gtgtcgacgc ggcgaagtgg
     2521 cgacaactcc tcgagtaact cccgatcgcc aaccgggtga ccgtatttcg gataggccgc
     2581 cgccgcgatc cacggctcgg tgttgcgata gaacgtgttc agatcgctca tcccgcagat
     2641 gctgatgccc gcggcgaaca gcgcggggtg gaacgtcagc gccgccaacg tcagatatcc
     2701 gccgtaggac cagcccgcgc aagcgatgcg cgccggatcg gccagttcgt tgtcgacgag
     2761 aaatcgcacg cactcggcga cgtcgtcgat cgcggcgaac cgcccctcct tgtcgtcggc
     2821 gtgcatgaag gcgcggccga atccgcccga gccccggaca ttcggtgtca gtaccgtgat
     2881 tccggcgttg agcagatgcg ggaagatctc gttgtagccc ggtcgcgcct ggccctccgg
     2941 cccgccgtgc agatacacca acgctcccgt tgcgggggcg cctgccggcg gccggtacag
     3001 ccacgccgtg aagcgcaaac cgtcgcgagc ggtggtggcc tcgagcgttg ggcccacggc
     3061 gaatccgctg accaccgggc cgcggctggg ttcacgatcg atccgctgcc attcccgcga
     3121 ccgcggttcc acgagttcga ctgtgcgcgg cagcgacggg ccctcgaccg tgagcgcgac
     3181 catcgacccg cccgcgctga tcgacagctc actggcgacc gtcgccggca acggaatcgc
     3241 ctccgacagg gttttgtccg tgtactcgag gatctgtaat tcgctgcgcc cctgaatgtt
     3301 ccacaacagc gccaccgtgc tgaggtcgtc gctgatgacg aattcgtcga ggtcgcaatc
     3361 cggccgttcg gccacaacga aataggacac gccgtcagcg gtcgcggtga cctccagcag
     3421 tcgggcgtgt tcacagccgt tgtcactgcg gatcaaagcg cggacgtagc cctcggcgct
     3481 gttcacgtcg taggtcttcg ccgggtggta gagccgcatc tcttctccgg ttggcccgaa
     3541 acggatccgg cggggctgat gatcgtcgag gatgacgccg acgtcggtgg tggatccggg
     3601 atccgatggc agcaatgcaa tctcggtgag accgtgcagc ataatcagct cctggtagcc
     3661 gcgcggcccg acccgaacca gcgacgctcc gccccatgca tccaccagtc gtccgcccga
     3721 gcgacggtcc agcacaatgc ggtcgccgtt cgcggggtcg atcaggcagg actggccgac
     3781 tccgtcttcg cccgtcagcg tggccgccac ccgtggccca tcccagctga ccaattcggc
     3841 catgccggcg tcctcgtaat cgatgcgccg ggccatccgg tcgtcgggat cggttgtgac
     3901 cacccagatc tgagagcgct cgcttccctc gggcgccacc tggcacgcca gccagtgacc
     3961 gtccgcagaa tggatgacgc gttcgaccgg accctctacc ggcaacacca cgtcacgtga
     4021 cgaactggcc cgccaccccc gcagaaaccg ttgcaccgca cggggatagc cgccgtcgtc
     4081 gacgatgtgc gcgaaggcgg tcgcgtccgg ggacagcgac gcgccgtaat tcgcgcgcac
     4141 ggagctgtct gctcttcgcg cgagcggctc atcgcccata cggattaacc ctccatgctc
     4201 tgcagccaca tctccagtac cccaagctgc cacagcgagt tcgcgccgag ggtggtgcgg
     4261 ccctcgttcg gcgcgttcag cagcgtctcc agcgtgtccc gtcggtacag gccgcgcgcc
     4321 cgggccgcgt cgttggtcag cgcgtcacgc acgaggtcga ggaccccacc gtcgaggtgg
     4381 cggattcccg gcaccgggaa gtaccccttg gtgcggtcga tgatctcggc gggcagcagc
     4441 gatcgcgacg cgtctttcag cacgcccttg ccgccggatg ccagcttgag gtccggcggg
     4501 caggtggcgg ccagttcgac gaagtcgtgg tcgaggaacg gaaccctggc ctccagaccc
     4561 cacgccatcg tcatcgtgtc gacacgcttc accgggtcgt cgaccaacat cacctcggtg
     4621 tcgagccgca acgccgcgtc gaccgcggtg tcggcgcccg gggcggcctg gtgcgcagtg
     4681 acgaattcgc ggctcacgtc ggtgtcgacg cagtactcgg gggcgaagat cgacaccacg
     4741 tcggagtggt cgcggtcgaa gaagactttc gcgtaggcat cggtggtctg gtcgcgggcg
     4801 atgttggcca gcggcggata ccagtcataa ccggccagga tctcgtcggc accctgcccc
     4861 gactgcacca ccttgatcga cttgctcact tcctcagaga gcagatagaa cgccacacag
     4921 tcgtggctga ccatcggttc actcatcgcg gcaatggttt tcggcagcgc gggcagcagc
     4981 cgggaattgt cgatgtggat cttgtggtgg tcggtgtcga aggccttggc caccatgtcc
     5041 gagtagtagt actcatcacc ggactggccg cccgcggagt cgaatccgat gctgtacgtg
     5101 gcaaggccgt gctggccctg ctcagccaac agcgccacca ccaccgacga gtcgatgccg
     5161 cccgacagca gcacccccac cgggacgtcg gccaccatcc ggcgcgacac cgcgacgcgc
     5221 aacgcgtcga tcagagcgtt ctgccagtcc tgcgtcgacc aatccttgcg accgggatca
     5281 cggcggaatt ccggtgacca gtagacggtg tcggtgtagc gaccgtccgg ctgaatcacc
     5341 cgcacagtcg ccggcggcag cttgcgcacg ccgcggtaga tggtccacgg agtgggaacc
     5401 accgcgtgaa aactcatgta gtgatgcagc gcaacccgat ccaggtcggt gtccacaccg
     5461 ccggcgtgga gcagggcgcg taccgacgac gcgaaccgca gcttgcccgg cgactcggcg
     5521 atgtagagcg gcttgatgcc caaccggtcg cggccgagag tcaccacgcc cgaggcgcgg
     5581 tcggcgatcg cgaacgcgaa catgcccttg aaccgctcga cgcaagccgt cccccatttg
     5641 tgaaacgcct tgatgatcac ctcgctgtcg gcggtcgaga agaagcggta accggccgcc
     5701 tgcagttcgg cgcgcaactc cttgtagttg tagatgcagc cgttgaacac cagcgtcagc
     5761 ccgagctcgc tgtcgaccat cggctgcccg ccgcacgcgg acaagtcgat gatcgacagc
     5821 cgccggtgcc cgaatgccac cggcccgtgc gccactagac cgtccgaatc gggcccgcgg
     5881 gacagcatcg cctcggtcat ccgcgacacc gcggccacgt cggcggaccg cccgtcgaat
     5941 cggatctctc cgcagatgcc gcacatgtcc gctagcctcc cccatcgtct tccatgagta
     6001 tccacgtgtc cttgctgcta cccccgaacg acgaattgac gatgcgggat ccacggctgg
     6061 ccacccgggt caacgccgcg ggggcgacgt gcgcggtggc ggagccgtca gccgcgccgc
     6121 gcaggtgaac gaagacacgc aggtcgacat ggtgcggaaa catgccctcc ccatcaaagg
     6181 ttgggtgtgt ggacaattcc accaactcct gggcgatgaa gcgctccggc tgggccagca
     6241 gttcgcgtcg tcgcgccgca agctccttct cggaggcgtc cggaccgatc agcacaccgc
     6301 cgccgccaag cccgtcgatc ggcttgacca ccaggtcggc gaggttgtcg agcacgtagt
     6361 cgcgctgagc ccgttcggcg cagatccacg tcggcacctg cgccagtttc ggcttctcgt
     6421 cgaggtagta ctcgatcatc gccggaacat aggcgtatat ggccttgtcg tctgccacgc
     6481 cgttgccgag ggcgttggcg atggtgagcg ttccgtcagc caccgcgcgc acaagaccgg
     6541 ggcgtagcgg cgcgccgtca tggccggtcg acgacagcag catgtcttcg tccatccgca
     6601 catagaggac gtcgacacgg cgcagttctg tgccgacatg acgcaccacg cgcccgtcgc
     6661 gaatcgacaa atccgacggg tgcaccaggg gcacgcccat ctcctcggcc aggaaggtgt
     6721 gctcgaacca ggcggagtcc tcccatccgg cggagatcag cgcgactttc gcgtcgtcgc
     6781 ctccgtgcgg tggggcggct gcccgcaggg tgtcgcagag catctgaagc gcctcgtcgc
     6841 tcttggtcgc cggcgccggc ggatgcagct cgggcaggtg cctggcgagc agtcgacggt
     6901 tcattaccgc gtaccccatg ccggacggca cccgcaagtt gtcctcgagc accatccagt
     6961 gaccgggccg gtcgcacacc aagtccgtgc cgctgatgtg gacgtgcacc cggtccgcgg
     7021 cgagcctgcc ggtcgaccgg aacccgggtg cccggtcgat ggcgtgcacg ccgagaaggc
     7081 cgtcgcggat gatctgctgg tccgaataga cgtcgcgcaa gaacgcgtcg agcgcgcgcg
     7141 cccgctggcc gagtcccgca cagagctgtg cccactcgtc tgcttcgacc atccgtggca
     7201 ccagatccag cgggaacagc tgtgcgcggt tctggccggt gacgcgaaac gtgatgttgt
     7261 cggcgcgctg ctcctgttca atgaggcctt ctcgggcccg cagcgccgcc acacctagac
     7321 cggcgatggt gtcaagaacc gtccgataac gagttcgagg ttgaccgcgg tcgactgcct
     7381 cgtcataggc gagagcctcg cagtcgtcca gctcggtgtc tgactgatac ccgaacagca
     7441 gagtcggatc gtcgccggcg gttgtcgcgg cactcggcca gcggccggcg gtctccgcga
     7501 tgagctggtc gacgacgtcg gtgagcctgc cccggcggcg caacgccctg cgctgccgcg
     7561 cggccgagct tcccgcggcg agcacacgcc ggctcagctc gtcgacgatc tcccagtcgc
     7621 cggtcgctcg gagctgcggc ttcagcgact cgacgagctc ggtgacgacg tcgggcgccg
     7681 gcctgctgcc cggcctcgtg gcatcgacga gttctccctc cagccccgat cgggccgccc
     7741 gccagatcgc ggcacggctg agtgtcgcag ggatgtcggg cgtcgggtgg ccttgccgca
     7801 gcgcaacgac ctcacgctcg accagggcgc gatacaaacc agcgatcaac acgatcgtgt
     7861 ccaccgaggg gcagctgtcg caaacccgaa gctccaacgt cggagcggac gccgatggcc
     7921 ggacgtcgaa gtacaccatg cccacgtcgg cgatcacgcc ggtggcgacc aggtcttcca
     7981 ccagcgcgtc gtactcggcg gccgtttcga caggggcggc gggccccgtt gtcggccagc
     8041 gctgccagac cagagtgcgg acgctcgagt agccggtgtc ggagccgtcg gaccagaacg
     8101 gcgagctggc tgacaacgcg agcagggtcg gcaggtgagg cgccagtcga tgcgctaccg
     8161 ctaccgcctc gtcacggtcg tcgacaccga cgtgcacctg agtaccgcag atgagttgct
     8221 ctcgggccag cagctggtag tcggccagca tccttcggta gcgagccgtc cgcgtcacct
     8281 gcatctcggc gggcaccgac agtggcacgg ccccggcggc gacgacaccg atgcccagtt
     8341 ctgccgcggt gtcgacgagc acgcagcggc ggtccacgag ttcggtcctg agcccgtcga
     8401 gtgtgtcgac gactctggtg ttcgtctcga cgacgcagcg ctgaagctcc gctacatagc
     8461 cgtccggcag ccgtttgagc aactcagggc cccgggcagt gagccgccgt gtgcgcaggt
     8521 cgacgaggtg aaactcctct tcgattccca gagtccgcgc tgattcaggt cccatccggc
     8581 cacgctatgg ctgtgcagtt acgggtgtgt aaatagcggg cccgctcaca cccgacccgc
     8641 cgcaccgtcc gcaggtaccc tcgcagccat gaggtggata tgaccgattc gccgcggccc
     8701 ggccagtcgg accccatcaa tccctaccac gacccgtacc aggcatatcc ggatcccgcc
     8761 tatgcgggca gtgcgccact cggtcccagc tacgccccga cgtccatgcc gtcgaatccg
     8821 gcgccgacgc aaccgctgcc gcaatattgg acgcagacat atccgcagac cgggtccatg
     8881 ccgcccgtcg atctgccgcc cgaaccgcct cgcggaccgc ggccctggct gtgggcgatc
     8941 gcgggtttct ccgtcgcggt ggtcatcggg ctggtcgtct gggtggtgtt cgtcaacacc
     9001 aatccgtcgc ggcaggagac gtcgattccg gcgatgcccc agtcgagcac cagccgcaca
     9061 ccgccgcgca ccgcgacgac gacgcccgcg ctgccgtcga tcctgcccat tcccctaccg
     9121 acactgacgc ttccgaatcc gaccaccagc ccgaacacct ccggagagac cgagccggtg
     9181 ctctacgagg tcaccggaga gggccgggcg atcaacatca cctacatcga caacggtggc
     9241 gtgatgcaga cggagttcaa cgtgctgctg ccgtggcaga aggacgtgga cttgcccaag
     9301 cccgcgaagg actcggcgag catcaccgtg atcaacgccg gccgcaacgt cacctgcaag
     9361 atcagcatcg gtggcgtgca ggtccaggag cgcaccggcg cgctgctgac cgtctgcagt
     9421 ccgtccggct agcggcgctt cggcgcgtgc accagcagca tcgcgacgaa tccggctgcc
     9481 agcacgacga gaagaccgcc cattccggcg cggtcggcgc cgaacgcgtc cacgaacgtg
     9541 gagaacatca gaggtgcgag gaatatcacg gcacgacccg tcgtggtgta gagcccgaat
     9601 accacgccct ccttgccgcg ggccgtcatc cgcagcagca gtgtgcgtgc cgacgagttc
     9661 gtcgggccga cgaacaggca cagcagcagg ccgagcaccc agaacgccgt ggcgcccgac
     9721 agcgtcatca gcgtcgcgcc caggacgatc atcgacgcca gcgagcctag gatgacggtc
     9781 ttggagccga tgtggtcgtc gagcagcccg ccgacgatcg ccccgacggc ggccaccacg
     9841 ttggccacca ccccgaacag caacacgtcg gccggggata ggccgtacac ggtggcggcg
     9901 agtaccccgc cgaatgtgaa cacccccatc aacccgtcgc gaaaaaccgc gctggccaac
     9961 aggtagtaca cgacgttgcg gtcgcggcgc cattcgccga cgatgtccga ccacagcttg
    10021 cggtaggcgc cgaacaggcc gatcttcggc gggatcgcgg cgtcaaggtc gtgcaccggg
    10081 ggcggcgcga tgagcacggg cagcgcgaac gcggccatcc acgccgcggt catcagcatt
    10141 gccgcacgca cgttctggcc atcggccgtt gggatgttca gcagcccggt cgtcggcccc
    10201 tcgccggcga tgaacccgac gtagaccacg aggagcagca ccatgctgcc gacataccct
    10261 gcgccccaac cgaatccgga aatacgaccg gatgtctccg gtgtcgccag ctgtcgcagc
    10321 atcgcgttgt agggaacact ggcgaggtcg ctgcacgcgg cggccagcgc gagcagcacc
    10381 agcccggccc acaggtagct ggggtcgtcg cggatcaggc tcatccccga tgtcagcagt
    10441 accaaaccgg cggtgaggaa gccaagcgtg cgccgtcttc gctgcggcgc gtccacccat
    10501 acgccgagga cgggagcgag cgatgcgacc accacgcccg cgagcgctag cgcgcggccc
    10561 agccagctcg ccggtgatgt gccgcccggc agatctgcgc cgacggtgcc ggtcaggtag
    10621 acattgaaca cgaacgtgac gacgaccgcg ttcaggcccg ttgacccgca gtcccagagt
    10681 gcccaggcca gcacgcgtga gcggggactg cccatggtgg ccgagcctat cggccgactg
    10741 tctacgatct tcggcatgcc agtacccgca cccagtcccg acacgcgcgc cgtcgtcacc
    10801 ggagcatccc agaacatcgg cgaagcgctg gccaccgagc tggccgcccg cggccaccac
    10861 ctgatcgtca cggcccgccg cgaggacgtg ctcaacgcgc tggcggcgcg gctcaccgac
    10921 cgctacggcg tcacggtcga ggtgcggccc gtcgacctcg tcgacccggc cgaacgggcg
    10981 aagctggccg acgaactggc gtcgcgcaac atctccatcc tgtgcgcgaa tgcgggcact
    11041 gcgacgttcg ggccggtcgc gtcgctcgac ccggcggacg agaaggcaga ggtacagctg
    11101 aatgtgcttg gcgtgcatga ccttacgttg gcggtgctgc ccgggatggt atcccgcgga
    11161 gcgggcggta tcttgatttc cggttccccg gccggcaatt caccgatccc gaacaacgcg
    11221 acctatgcgg ccacgaaggc gttcgtcaac acgttcagtg agtcactacg gggagaactg
    11281 aagaaggccg gcgtgcatgt cacgctgctg gcgccgggac cggtacgcgg tgatctgccc
    11341 gacgacgcgg aggcgtccct cgtcgacaag ctggtgccgg aattcctgtg gatctcaacg
    11401 gaacacaccg cgaagctgtc gctggacggg ttggagcgca acaagatgcg cgtcgtaccc
    11461 ggagtgacgt ccaaggcaat gtcggtggcc agcggctaca cgccgcgcgc gatcgtcgcg
    11521 ccgatcgtcg gcgcgttcta caagaagctc ggcggctagc cgccttgcgc cgaaaccgaa
    11581 ttccaggtcg tggtttcggc ggtttcacga ccctcgtttc actctcggcg aagcaagccg
    11641 atcagcggcg acgacggccg gtgccgaaga tacttcgggt gatttcgcgc ccgatcaccg
    11701 tgccggctga gcgcatggcg ctcttgaacg ccgagctctg cagaacgtcc tccaccatgc
    11761 cgccctcggc cgcgtccggc gtcttcatct tgcccgagac gctggcgccg ccggcctgcg
    11821 caggttccgg cggcggtgcg agcttggcgg ccaaccgctc gtaggccgag tcacggtcga
    11881 tggtctggcc gaaatacggc tgcagcgcac tggttttggc cgccgccgcg atcgcgtcgg
    11941 gcccgatcgt gtccatcagc gagcgcggcg agcgcatccg cgtccacgcc accggtgtcg
    12001 gcgcgccttt ctccgacagg acggtgacga cggcctcgcc gatgccgagc gacgtcagcg
    12061 ccgactcgag gtcgtacacg ttggtcttcg ggtacgtgcg gacggtcttc gacagcgcct
    12121 tctgatcgtc cggggtgaac gcgcgcagcg cgtgctggac ccgcgcgccg agctgcgaca
    12181 gcacgtcgtt gggcacatcg gtgggcagct gcgtgcagaa gaacacgccc acgcccttcg
    12241 agcggatcag cttgacggtc tgctccacct gctcgaggaa cgccttcgac gcgtcggtga
    12301 acagcagatg cgcctcgtcg aagaagaaca ccagcttggg cttgtcgagg tcgccgacct
    12361 cgggaaggaa tgtgaagagg tcggccagca cccacatcag gaaagtcgag aacatcaccg
    12421 gccgcgcggc ttgggcgccc acctcgagca gcgtgatgac gccgcggcct tgcgcgtcga
    12481 cgcgcatcag agcgttcgga tcgagttcgg gctcgccgaa gaacgtgtcg gcgccttcgg
    12541 cttcgaggtt caccagcgcc cgcaggatca cccccgccgt cgtcgacgac actgcgccga
    12601 gcgctttgag ctcgggcttg ccctcgtcgc tggtcaggaa ggtgatgacc gagcgcaggt
    12661 ccttcgtgtc gagcagcggc aggcccttct gatcggccca gtgaaagatc aacccaagtg
    12721 tcgactcttg ggtagcgttg agccccaaca cctttgacag caggataggc ccgaagctgg
    12781 agatcgtggc ccgcaccggc acgccgatgc cgccggtgcc cagcgacagg aactcgacgg
    12841 ggtacgcggc cgggacccag tcgtcgccgg tgtccgtggc gcgggccgcc gtcttgtcgt
    12901 tggccactcc gggtcgcgac agcccggaca ggtcgccctt gatgtctgcc atcatcaccg
    12961 gcacaccggc agcggacagc tgctcggcca tcacctgcag cgacttcgtc ttgccggtgc
    13021 cggtggctcc cgcgaccagg ccgtgccggt tgaccgttgc caggggaatg cggatgcggg
    13081 cggtcgggtc tacggtgccg tcgacgacga ccgtcccgag ctcgagcgct tggccctccg
    13141 ccgcgtagcc ggcggcgatc tgctgggcgg gcggcggtga cgattctctg ctcatcgtca
    13201 ccagagctta gtgttgagcg ctgtgcggga cgaactggtg tgggtcgact gcgaaatgac
    13261 cgggcttgac ctggccaagg accggctcat cgagatcgcc gcgctggtca ccgacggcga
    13321 gttgaacgtg ctcggcgacg gcgttgacgt ggtgattcac accgacgagg acgccctgac
    13381 ggggatgatc gatgtcgtca ccgaaatgca cacccgctcc gggctgaccg acgaggtccg
    13441 cgcgtccacc gtcgacgttg ccgaggcgga ggcgatggtg ctcgactaca tcaaagcaca
    13501 tgtgccgcag gcgaaaatgg ctcccctggc gggaaactcc atcgccaccg atcgcgggtt
    13561 catcgcccgc gacatgcccg cgctcgacga ctacctgcac taccggatga tcgacgtcag
    13621 ctccatcaag gagctgtgcc ggcggtggta tccgcggatc tactacggtc agcccgaaaa
    13681 gggcctcgcg caccgggcgc tcgccgacat tcaggaatcg atccgtgagt tgcagttcta
    13741 ccgccgcacc gcgttcgttc cgcctccggg gccgtcgagc accgagatcg cggcggtcgc
    13801 cgccgagttc aacgccgcaa cgagcacgtc cggggatatc aattcggctc cagaggccac
    13861 gagcggctaa tatcgccatc gccgggatca ttcgggtcgg cggcgatggt gagtgtagtt
    13921 cagctggtag agcaccaggt tgtggtcctg gaagtcgcgg gttcgagtcc cgtcactcac
    13981 cccaacagct cagagggcat tttctgttcg ccctggactt tctcttgctt ggcaccttgc
    14041 acccttagcg caaccttgcc gtcccacgct cggaagacgt ggcaaaactc gacgagcatt
    14101 cgtaagtcga tagaactgac ctcaatagcc cgtgggaccg caggttgaaa acctgccggg
    14161 gttggcgcct tgacctgcac agaccacgca accggtgtga tttctaccta tccccctatc
    14221 cattcgggtg cgaactgagg ccgcgctaga gccagccttc ggcgtgcatc ttggagtcga
    14281 acgtgtgcgg agcgtcgtgc cgacgaaggt ccg
//
LOCUS       DASWBF010000117         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_735267,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000117 DASWBF010000000
VERSION     DASWBF010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735267"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VF874_04065"
     CDS             <1..180
                     /locus_tag="VF874_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175293.1"
                     /translation="WVVESYDRDAATVYSPAEQFEQVGALLSAADAAAWAGVLFSPKF
                     RLICVSAVRDRVGRP"
     gene            388..1449
                     /locus_tag="VF874_04070"
     CDS             388..1449
                     /locus_tag="VF874_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9175294.1"
                     /translation="MIVIGVDPHKSTHTATAVDPVTNTDLGSLRVEATLADYQRMLAW
                     AKQWPQRQWAVENAEGLGHHLAQWLVARGEVVLDVPTTSTARVRELSRGARRKNDRID
                     AAGAACVAAAQGDARPVVPEDHVDALALLDERRMNLSQNRTRAINQLHRLLRELLAGG
                     VPSDLTAAKAAAALRAFRPHSTPERIRLQLCRELIADVRRLDQQLADNQTQAARLLDE
                     HGTRLREIDGVGPVLAARILGRTGHVTRFATAAAYATYNGTAPVEVASAEHQCHRLNR
                     YGDRQLNSAIHTIAIVQLRMPDSAARRYYDRKIADGKAPRATLRCLKRHLSNQLWRTM
                     IADEHRHRDKPNNQSARAS"
BASE COUNT          286 a          530 c          531 g          272 t
ORIGIN      
        1 tgggtcgtgg aatcgtacga ccgcgacgcc gcgacggtgt actcgccggc ggaacagttt
       61 gagcaggtcg gcgctctact gtcggccgcc gacgccgccg cctgggccgg cgtattgttt
      121 agcccgaagt tccgacttat atgcgtgtca gcggttcgtg atcgggttgg ccgcccgtag
      181 cgtgccgtag cgaaccggag ggcctgactc gcgaagttcc tgtcacctgg tattcgggtt
      241 gactctcagg gggtgtgtcg agccctccgg ttcgcgttcg cggcgtgact gaagagaggc
      301 gtgaccacaa gtacgtgcca ggtggcttga agtcggattg tccgccgcgg atcccccgtt
      361 acagcgcgag aagggaatcc gttgtccatg atcgtcatcg gcgtcgaccc gcacaagagt
      421 acgcacaccg ccaccgcggt ggatccggtc actaacaccg atctgggttc gctgcgtgtc
      481 gaggccacgc tggccgacta ccagcggatg ctggcctggg ccaagcagtg gccacaacgg
      541 cagtgggcgg tggagaacgc cgaagggttg ggtcatcact tggcacagtg gcttgtggcc
      601 cgtggcgagg ttgtgctcga cgtgccgacc acctcgaccg cgcgtgtgcg cgaactgtct
      661 cgcggcgcgc ggcgcaaaaa tgaccgcatc gatgcggctg gggctgcgtg tgtggctgcg
      721 gctcagggcg atgcccgtcc ggtagtaccc gaggatcacg tcgatgcgct cgcgctgctc
      781 gacgagagac gaatgaacct gtcgcagaac agaacccgtg ccatcaacca actgcaccgg
      841 ctgctgcggg aactgctggc cggcggagtc ccgagcgacc tgaccgccgc caaggctgct
      901 gcggcgttgc gcgcgttccg gcctcacagc acccccgagc ggattcggct gcagctgtgc
      961 cgcgagctga tcgccgatgt ccgccgcctc gaccagcagc tcgccgacaa tcagacccag
     1021 gccgcacggc tgctcgacga gcacggcacc cgactgcgcg agatcgacgg cgtcggcccg
     1081 gtgctggcgg cccgcatcct cggccgaacc ggtcacgtaa cccggtttgc taccgcggcc
     1141 gcctatgcca cctacaacgg caccgcccca gtcgaggtcg ccagcgccga acaccaatgc
     1201 caccggctca accgctacgg cgaccggcaa ctgaactccg ccatccacac catcgccatc
     1261 gtccagctcc gcatgcctga tagcgccgca cgacgctact acgaccgtaa gatcgccgac
     1321 ggcaaagcac cacgagcgac gctgcgctgc ctcaaacgac acctatccaa ccagctctgg
     1381 cgaaccatga tcgccgacga acatcgccac cgcgacaaac ccaacaatca atccgcgagg
     1441 gcctcttgac aaacagagag gcaccccctg gtgtgttgac gtgttggggt gttgacctga
     1501 ggttttgggt tgtggctggg tcggtgtact gactacgtca ggccgtgacg gggtgagaca
     1561 tcgagtcgtt cgaaggctcg ccgctgtcgt ggggtggggg tggtgagcag ggtgaatgc
//
LOCUS       DASWBF010000118         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_272331,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000118 DASWBF010000000
VERSION     DASWBF010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272331"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2511)
                     /gene="dnaE"
                     /locus_tag="VF874_04075"
     CDS             complement(<1..2511)
                     /gene="dnaE"
                     /locus_tag="VF874_04075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9175295.1"
                     /translation="MDSSMRSFVHLHNHTEYSMLDGAAKIAPMLAEAQRLDMPAIGMT
                     DHGNMFGASEFYSEATKAGIKPIIGIEAYIAPGSRFDTRRVSWGDPGQKDDDVSGSGS
                     YTHLTMVAENATGLRNLFKLSSLASFEGQLGKWARMDAELIAEHAEGIVATTGCPSGE
                     VQVRLRLGHHREALEAAAKWREIFGPDNYFLELMDHGLGIERRVRDGLLEIGRTLGID
                     PLATNDCHYVTRDAAHNHEALLCVQTGKTLSDPNRFKFEGDGYYLKSAAEMRQLWDDQ
                     VPGACDSTLLIAERVQPYDDVWQPRNRMPVFRVPDGHDEASWLRHEVDAGLRRRFPGG
                     LPDGYAERAAYEIKVICDKGFPSYFLIVADLVGYARSVDIRVGPGRGSAAGSLVAYAL
                     RITDIDPIEHGLLFERFLNPERASMPDIDIDFDDRRRGEMLRYASEKYGHDRVAQVIT
                     FGTIKTKAALKDSARIHFGQPGFSIADRITKALPPPIMAKDIPLSGITDPSHERYKEA
                     AEVRGLIDTDPEVRTIYDTALGLEGLIRNAGVHACAVIMSSEPLTDVIPLWKRPQDGA
                     VITGWDYPSCENIGLLKMDFLGLRNLTIIGDAIDNIKANKGIDVDLESLPLDDKPTYE
                     LLGRGDTLGVFQLDGGPMRDLLRRMQPTEFEDIVAVIALYRPGPMGQNAHNDYADRKN
                     GRQPVKPIHPELAEPLVDILAETYGLIVYQEQIMRIAQKVAGYSLARADILRKAMGKK
                     KREVLDKEYEGFAEGMRGNGFSAGAIKALWDTVLPFADYAFNKSHAAGYAMVSYWTAY
                     LKANYPAEYMAGLLTSVGDDKDKAAVYLADCRKLGITVL"
     gene            complement(2574..2984)
                     /locus_tag="VF874_04080"
     CDS             complement(2574..2984)
                     /locus_tag="VF874_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175296.1"
                     /translation="MRPVQEVRAPDQCPPPGGPPRLLVSSDAVTVCDVPRSAAYVLGP
                     QAIELQLTQVDSVKSPTSEFYVVRVTMQPVSAAAFADLTARYVGQQLAFVRDDVVVSA
                     PQITAPINSQVLEISGQLTAQQADEVARLLRQPN"
     gene            complement(2995..3159)
                     /locus_tag="VF874_04085"
     CDS             complement(2995..3159)
                     /locus_tag="VF874_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175297.1"
                     /translation="MIDQRRGEFLRKLFAIVALIGTLAVVVAIIGGVWMRIAGDDQQV
                     RTGSKHTAHV"
     gene            complement(3200..>3548)
                     /locus_tag="VF874_04090"
     CDS             complement(3200..>3548)
                     /locus_tag="VF874_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX9175298.1"
                     /translation="ALQVLGNGHFVGFGVGHTTLLALAGPVTAIPLLLFAAAAQRLPL
                     ITLGLLQYLNPGLQMAWGVLVGHEAMPTARWVGFTLIWAALAVLTLDWLTRRPAEIAD
                     ADVDSLAPKSSSR"
BASE COUNT          567 a         1169 c         1192 g          620 t
ORIGIN      
        1 cagcaccgtg atgcccagct tgcggcagtc ggccagatac acggcggctt tgtccttgtc
       61 gtcgcccacc gaggtcaaca ggccggccat gtactcggcc ggatagttgg ctttcaggta
      121 cgcagtccag taggacacca tcgcgtatcc ggcggcgtgg ctcttgttga acgcgtagtc
      181 ggcgaacggc agcacggtgt cccagagcgc tttgatggcg ccggcggaaa aaccgttgcc
      241 gcgcatgccc tctgcgaagc cctcgtactc cttatcgagc acttcacgct tcttcttgcc
      301 catcgctttg cgcaggatgt cggctcgggc cagtgagtac ccggcgacct tctgcgcgat
      361 gcgcatgatc tgttcctggt agacgatcaa gccgtacgtc tcggcgagga tgtcgaccag
      421 cggctcggcc agctcgggat ggatcggctt gacgggctgc cgcccgttct tgcggtcggc
      481 gtagtcgttg tgggcgttct ggcccatcgg cccgggccgg tacagcgcga tgaccgcgac
      541 gatgtcttcg aactccgtgg gctgcatgcg gcgcaacagg tcccgcatcg gcccgccgtc
      601 gagttggaac acgcccagag tgtcgccgcg gcccagcaac tcgtaggtcg gtttgtcgtc
      661 cagtggcagc gactcgaggt cgacgtcgat tcccttgttg gccttgatgt tgtcgatcgc
      721 gtcaccgatg atcgtcaggt tccgcaggcc caggaaatcc atcttcagca ggccgatgtt
      781 ctcgcacgac ggatagtccc agccggtgat caccgcgccg tcctgcgggc gcttccacaa
      841 cgggatgacg tccgtgagcg gctcggagct catgatcacc gcgcacgcgt gcacgccggc
      901 gttgcggatc agcccttcga gaccaagcgc tgtgtcgtag atggtccgca cttcgggatc
      961 ggtgtcgatc aggccgcgca cctcggcggc ttccttgtac cgctcatgac tggggtcggt
     1021 gatgcccgac agcggaatgt ccttggccat gatcggcggc ggcagcgcct tggtgatccg
     1081 gtcggcgatc gagaacccag gctggccgaa atggatgcgc gccgaatcct tcagcgctgc
     1141 tttggttttg atggtgccga acgtgatcac ctgcgcgacg cggtcgtgac cgtacttctc
     1201 gctggcgtag cgcagcatct cgccgcggcg gcggtcgtcg aagtcgatgt cgatgtcggg
     1261 catcgaggcg cgctccgggt tgaggaaccg ctcgaagagg agcccgtgct cgatcgggtc
     1321 gatgtcggtg atgcgcaacg cgtaagcgac cagcgaaccg gcggccgacc cacgaccggg
     1381 cccgacccgg atgtccaccg accgggcgta gccgaccagg tcggcgacga tcaggaagta
     1441 cgacggaaaa cccttgtcgc agatgacctt gatctcgtag gccgctcgct cggcgtagcc
     1501 gtcgggcagc ccgccgggga accgccgccg caacccggcg tcgacctcat gacgcaacca
     1561 cgaggcttcg tcgtgcccgt cgggcacccg gaacaccggc atccggttgc gcggctgcca
     1621 cacgtcgtcg tagggctgga cccgctcggc gatcagcagg gtgctgtcgc acgcgcccgg
     1681 aacctggtcg tcccacagct ggcgcatctc cgccgcggat ttcaggtagt agccgtcgcc
     1741 ctcgaacttg aagcggttcg ggtccgacag cgtcttgccg gtctgcacgc acagcagcgc
     1801 ctcgtggttg tgggcggcgt cgcgggtgac gtaatggcag tcgttggtgg ccagcgggtc
     1861 gatgccgaga gtgcggccga tctcgagcag cccgtcgcgc acccggcgct cgatgcccag
     1921 cccgtggtcc atcagctcca ggaagtagtt gtcgggtccg aagatctccc gccacttggc
     1981 ggccgcctcc aacgcctcgc ggtgatgccc gagccgcagc cggacctgca cctcccccga
     2041 cgggcagccg gtagtggcga cgattccctc ggcgtgttcg gcgatcagct cggcatccat
     2101 ccgcgcccac ttgccgagct gaccctcgaa cgaggccagc gacgacagtt tgaacaggtt
     2161 gcgcaggccc gtggcgttct cggccaccat tgtcagatgc gtgtaggacc cgctaccgga
     2221 gacgtcgtcg tccttctggc ccggatctcc ccaggacacg cggcgggtgt cgaaccgcga
     2281 gccgggcgcg atgtacgcct cgatgccgat gatcggcttg atgcccgcct tggtggcctc
     2341 cgagtagaac tcgctggcgc cgaacatgtt tccgtggtcg gtcatgccga tggcgggcat
     2401 gtccagccgc tgcgcctcgg ccagcatcgg tgcgatcttc gcggcaccgt ccagcatcga
     2461 atactcggtg tggttgtgca ggtgcacgaa ggaccgcata gaggaatcca taggctcgtc
     2521 agtctatggc gacccactga cgcttctcgg cgtgtcgccg gcgtgtgtct cggtcagttc
     2581 ggctggcgca gcagccgcgc cacttcgtcg gcctgctgcg cggtcagttg gccggagatt
     2641 tcgagcacct gggagttgat gggtgcggtg atctgaggtg cggagacgac gacgtcgtcg
     2701 cgcacgaacg ccagttgctg cccgacatac cgggcggtca agtcggcgaa cgccgccgcg
     2761 gacaccggct gcatcgtgac ccgcacgacg tagaactccg acgtcggcga cttgaccgaa
     2821 tcgacctggg tgagctgcag ttcgatggcc tgcgggccga gcacataagc cgcgctgcgc
     2881 ggcacgtcac agacggtcac ggcgtccgac gacacgagca gccgcggcgg ccctccgggt
     2941 ggcgggcact ggtcgggagc gcggacctcc tgcaccgggc gcaccggaag cggcttagac
     3001 gtgggcggtg tgttttgagc cggtgcggac ttgctggtcg tccccggcga tccgcatcca
     3061 cacgccgccg atgatcgcga cgacgacggc gagagttccg atcagcgcca cgatggcaaa
     3121 cagtttcctg agaaactccc cgcggcgttg gtcgatcatc cgggaagtct ggcacggcac
     3181 gccgtcagaa agtgacctgt caccgtgatg acgatttcgg agccagcgaa tcgacatccg
     3241 cgtcggcaat ttctgcgggc cgccgcgtca gccagtccag cgtgagaacc gcaagcgcgg
     3301 cccagatcag cgtgaaaccc acccaccgcg ctgtcggcat cgcctcgtgg ccgaccagca
     3361 cgccccacgc catctgcagg ccggggttga ggtactgcag aagcccaagg gtgatcagcg
     3421 gcaggcgctg cgccgccgcg gcgaacagca gcagcggaat cgcggtcacc gggccggcga
     3481 gcgccagcag cgtggtgtgt ccgacgccga atccgacgaa gtgaccgttg ccgagcacct
     3541 gcagcgcg
//
LOCUS       DASWBF010000119         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_472675,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000119 DASWBF010000000
VERSION     DASWBF010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472675"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..728)
                     /locus_tag="VF874_04095"
     CDS             complement(177..728)
                     /locus_tag="VF874_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9175299.1"
                     /translation="MMRDISKPIASLLSIRLHPKLIRAGGRFHLALLRRFRRAAFLGA
                     DTLVLTTRGRRSGQPRATPLYYVPHADRRYIAASFAGRDAPPNWYLNLLADPEVTVEV
                     AGDREGCHARVLSPREAAVIWPQLIASYPPFARYQRRTKRTIPVIELSRVDAGSRTPG
                     EYGRTVASPAVTLGDSPSGTGRS"
     gene            1298..1735
                     /locus_tag="VF874_04100"
     CDS             1298..1735
                     /locus_tag="VF874_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX9175300.1"
                     /translation="MTSPAFGDNTPIPAEYSCNGRNVPPPLRWENPPPGTESLALVVD
                     DPDAVGGLYIHWVVTGIPPTATGLVEGALPQGAQVSLNSGGKAAYLGPCPPAGTGVHH
                     YRFQLYALSKQLVLAPTTPAGQATQTIAGAALATASTVGLFSG"
     gene            complement(1804..2046)
                     /locus_tag="VF874_04105"
     CDS             complement(1804..2046)
                     /locus_tag="VF874_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175301.1"
                     /translation="MHKLAGSGITREEAREALRTTHGGWIGGSHVVDSDRARLVVAAA
                     TRSMLDAIHTLTLITAVVPIAAIVVAIVLIKPTAPR"
     gene            2247..>2452
                     /locus_tag="VF874_04110"
     CDS             2247..>2452
                     /locus_tag="VF874_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175302.1"
                     /translation="MTAGWKRTVTAVGGGLQLLWRIRLGWIMGHGLLVLTHVGRHTGK
                     QYRTVLYVQRYDDRSREATVISVWG"
BASE COUNT          416 a          815 c          817 g          404 t
ORIGIN      
        1 agccggctcg gccggcgccg cccaggtagc gggtgttctc gttcagcagg cagtgcgaca
       61 gcagcaccac ctggttaccg cggtcatcgt gcagccgacg cgccaagagc cgcgtgcggc
      121 gcgaggcccg ggcggtcacg gtgcgtccga accagtgagt ttcgttgcca cagtggctaa
      181 ctccgtccgg tgccgctggg gctgtcaccc agcgtgacag cggggctcgc aacggttcgg
      241 ccgtactccc cgggggtgcg gctgccggcg tcgactctgc tcagctcgat caccgggatc
      301 gttcgtttgg tgcgccgctg atagcgcgcg aacggcgggt agctggcgat caactgtggc
      361 cagatcacag ccgcctcccg cgggctcaat acccgcgcat ggcagccttc gcgatcaccg
      421 gcgacttcga cggtcacctc cggatcagcc aggagattga gataccagtt gggtggggcg
      481 tcgcgaccgg cgaaggaagc ggcaatatag cggcggtcag cgtgtgggac gtagtacagc
      541 ggggttgcgc gcggttggcc gctacggcgg ccgcgggtgg tcaggaccag agtgtcggcg
      601 ccgagaaagg cggcgcgccg gaatcgccgg agcaaggcca aatggaaccg accgcctgcc
      661 cggatcagct tgggatgcag gcgaatactc agcaggctgg cgatcggctt ggagatatct
      721 cgcatcatgt gccccactca ccatgtgggc acgatcgatc gtgcccacct cctgagtctg
      781 cctccaccgc cttgctgcgg gcagagtctt tgggcctcgg cttggccggc acccaccagc
      841 gagcgtccga tcgggtgcgg gtcgtcactg cacctgggcc agtccggcga actacggcca
      901 tccaagccga tgccgcatct accgaaagcc ttggcgacga ggtcatttcg ctcgtagagc
      961 gcagcggctc cattggcggt gatgctgctc ttcatcgcgg cggacgagct gtccgtctcc
     1021 ttcccggtgt gctctctgct gggccgggca ccgatcgaaa tcgcgatggc gggctcgctt
     1081 ctatcacagc cgatgagcgc gtatacatag gtccatgaga ctggcgacgg ccaccatggc
     1141 ggtgctcttc ttcgcactca cagcgtgtgg cggcagtgag aagagtaaag agacatctgc
     1201 ttccagcact tcagcgcaac cgacgacgac cacggcacca gaccaagcga cgacgaccac
     1261 agcatcggcg ccgccggtcg cgccggcgcc gtttgcggtg accagtcccg ccttcggcga
     1321 caacacgccg atcccagccg agtactcctg caacggccgt aacgtacctc cgccacttcg
     1381 gtgggagaac ccgccgcctg gcaccgagtc cttggctttg gtcgtcgacg accccgacgc
     1441 cgtcggcggg ctctacatcc actgggtggt caccggcata ccacccacag ccaccggact
     1501 cgtcgaaggt gcgctacccc aaggagctca agtcagcctc aactccgggg gcaaggcggc
     1561 ctatcttggc ccatgcccgc ccgccggcac cggcgtgcac cactaccgct tccagctcta
     1621 cgcactgagc aagcagttgg tgttggcgcc gacaacaccg gcagggcaag ccacccagac
     1681 gatcgccggc gcggccctcg ccacggccag cacagtcggc cttttcagcg gctgaggcga
     1741 gcaccatcgg cgttctgatg caacgacatc gacgtcgatc gagcaggtcc tcgaagtcga
     1801 gcgttaccgc ggcgccgtcg gcttgatcaa cacgatcgcg acgacgatgg cagctatcgg
     1861 taccacggcc gtgatcagag tgagggtgtg aatggcgtcg agcatgctcc gcgtcgccgc
     1921 cgcgaccacc agcctggccc ggtcggagtc gacgacatgt gagccgccga tccaaccgcc
     1981 gtgcgtcgtc cgtaacgcct cgcgcgcctc ctcgcgggtg atgccggagc cggcaagttt
     2041 gtgcatgccg tcgctgatga cgcagatgtc ggcgccggaa aggtcgcatg ctttgggacg
     2101 atcggcccta ggtgctcgcg gctggtgcgc ggacgatggg tctcggcatg gagaacaccc
     2161 gcacacgcgt gggcgaactg gcacaagtta ctcgcgcgag tcgccgcact gtcacaccga
     2221 gaaaccctgc cggcgggagg tggccggtga cggctggctg gaagcgaacc gtcaccgctg
     2281 tcggtggcgg cctacagctg ctgtggcgca tccggctcgg atggatcatg ggccatgggc
     2341 tcttggtgtt gacccatgtc gggcgccaca ccggaaagca gtaccgcacg gtgctatacg
     2401 tccagcgtta tgacgaccgc tcgcgtgagg ccacggtgat ctcggtgtgg gg
//
LOCUS       DASWBF010000120         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_408105,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000120 DASWBF010000000
VERSION     DASWBF010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408105"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VF874_04115"
     CDS             <1..988
                     /locus_tag="VF874_04115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9175303.1"
                     /translation="AKAEVEIPSPYAGRVVERCGEAGDVLPVGSVLMRIGTKSAEPQA
                     RNPVLVGYGADDAMGTSRRSSRPRAAPAVRKLAKDLHVDLASLQPGSGGGGVITREDV
                     SRVSSVRARMAERMTLSRSQIPDAHASVDVDGTRLLQLCDRFSAMGADVTPFVLTLRL
                     LTIALTRHAVLNSTWVDGDDGPQVHVHHTVNLGVAVATDRGLLVPVVFDAQTKTTREL
                     ALSVMDLVRRARDGRIKPAELQGSTFTVSNFGALGLDDGVPVINYPEAAILGIGALKP
                     RPVAVDGEVVVRPTMRLTCVFDHRIADGAQVAQFLCELRQLIETPETALLDS"
     gene            complement(1130..1891)
                     /locus_tag="VF874_04120"
     CDS             complement(1130..1891)
                     /locus_tag="VF874_04120"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9175304.1"
                     /translation="MPDSVLFDVRDRVALITVNDPDRRNAVTGEISDGLRAAVARAEA
                     DTDVHSVIVTGAGSAFCAGADLSALGSAGSAAEEGLRRLYDGFLAVADCALPTVAAVN
                     GPAVGAGLNLALACDVRIAGPRALFDPRFQKLGIHPGGGMTWMLQRAAGPEVARAALL
                     FGMRFDAEAAVRHGVALSVADDPVAAAFELAAGPASAPRDVVIATKKTMRATISPGAV
                     DSDQHAFAVRTEIGPQAQSIRSPEFAERLAAARRR"
     gene            1987..>2494
                     /locus_tag="VF874_04125"
     CDS             1987..>2494
                     /locus_tag="VF874_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003404760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HEX9175305.1"
                     /translation="MKLAAWARSVGVHPQTAYGWVREDRMPVPFRRLPSGTILVDVAA
                     AGDERAVLYARVSSHDQRADLDRQVSRLTEWATDAGIQVGQLVAEVGSGLTGKRPKLA
                     RILSDPSATVIVVEHRDRLACFGMEHLRAALAAHGRRIVVVDDGETTDDLVRDMIEVL
                     TSMCARLYG"
BASE COUNT          384 a          788 c          898 g          424 t
ORIGIN      
        1 ggccaaggcc gaggtcgaaa tcccgagccc gtacgcgggt cgcgtcgtcg aacggtgcgg
       61 cgaggccggt gacgtgctgc cggtcgggtc ggtgctgatg cgtatcggca cgaaatccgc
      121 agagccgcag gcgcgtaacc ccgtgctggt gggctatgga gcggacgacg cgatgggcac
      181 cagtcggcgg tcatcgcgcc cgcgagcagc gcccgccgtc cgcaagctcg cgaaagatct
      241 tcacgtagac ctcgcgtcgc tgcagcccgg ttcgggcggc ggtggggtga tcacccgcga
      301 ggacgtcagt cgcgtttcca gcgtccgcgc ccggatggct gaacggatga cgctgtcgcg
      361 cagtcaaatt cccgatgcgc atgccagtgt tgacgtcgac ggtacgcgtc tgctgcagtt
      421 gtgtgatcgg ttctcggcga tgggtgccga cgtgacgccg ttcgtgttga cgttgcggtt
      481 gctcacgatc gcgttgacgc ggcacgcggt gctgaactcg acgtgggtcg acggcgacga
      541 cgggccgcag gtgcatgtgc accacaccgt gaacctcggc gtcgcagtgg ccaccgatcg
      601 aggcctgctc gtgccggttg tctttgacgc acagacgaag accacgcggg aattggcctt
      661 gtcggtaatg gatttggtgc gcagggcccg cgacggccgc atcaaaccgg cggaacttca
      721 gggctcgacg ttcacggtgt ccaatttcgg tgcgctgggc cttgatgacg gcgtcccggt
      781 gatcaactac ccggaggccg cgatcctggg gatcggggca ctcaagccgc ggccggtggc
      841 cgtcgacggc gaggtcgtgg tgcggccgac gatgagattg acctgcgtgt tcgaccatcg
      901 aatcgccgac ggtgctcaag tcgcccagtt cctctgcgag ctaaggcaac tcatcgaaac
      961 cccggagacc gcactgctcg attcgtgacc gcgagcgtgc gtgtctgtac cccgacacgc
     1021 cgcagaacca ccacacactg cgcacactcg tccccgcgcg agggtgcatg ttcgcagact
     1081 gcggcccggc gtgttggggt acagacatgc acgctcgcgc caaagagggc tagcggcggc
     1141 gggcggcggc gaggcgttcg gcgaactccg gcgaccggat cgactgcgcc tgtggaccga
     1201 tttcggttcg caccgcgaac gcgtgctggt cggagtcgac ggcgccgggg ctgatcgtgg
     1261 cccgcatggt ctttttggtc gcgatcacca cgtcgcgcgg cgccgacgcc ggaccggccg
     1321 ccaactcgaa cgcggcggcc accgggtcgt cggcgacact gagcgcgaca ccgtggcgca
     1381 cggccgcttc ggcgtcgaac cgcatcccga acagcaacgc tgcgcgcgcc acctctggcc
     1441 ccgccgcccg ctgcagcatc caggtcatgc cgccgccggg atggatgccg agcttctgaa
     1501 accgaggatc gaacaatgcc cgcggcccgg cgatccgcac gtcgcaggcc agcgctaggt
     1561 tcagcccggc cccgaccgcg gggccgttga ccgcggccac cgtcggcagt gcgcaatccg
     1621 cgacggcgag aaacccgtcg tagagccgac gcaggccctc ctcggcagcg ctgccggcgc
     1681 tgcccagcgc ggagaggtcc gcgccggcgc agaacgcgct gcccgcgccg gtgacgatca
     1741 ccgagtgcac gtcggtgtcg gcttccgcgc gcgccaccgc tgcgcgcagc ccgtcggaga
     1801 tctcgccggt gaccgcattt cggcggtcgg gatcgttgac ggtgatcagc gcgacgcggt
     1861 cgcggacgtc gaacaagacc gaatcaggca tctgtcaaca gtagtcaaag cgggggtgac
     1921 gaacggttga gcaactcgcg ggtgaatgtg cgtgtttgtg agtgaatgta tgtttggctg
     1981 gccgtcgtga agttggcggc gtgggcgcga tcggtgggtg tgcatccgca gacggcctat
     2041 gggtgggtgc gtgaggatcg gatgccggtg ccgtttcggc ggctgccctc gggcacgatc
     2101 ctggtcgacg tcgcggcggc gggtgacgaa cgtgcggtgc tgtatgcgcg ggtgtccagc
     2161 catgatcaac gcgccgatct ggatcggcag gtgtctcgtt tgactgagtg ggccaccgat
     2221 gccgggatac aggtcggcca gctggtggcc gaggttggtt ccggattgac cggcaagcgt
     2281 ccaaagctgg cccggatctt gtcggaccct tctgccacgg tgatcgtcgt ggagcatcgg
     2341 gatcggctgg cttgcttcgg aatggagcat ctgcgggccg ccctggccgc ccatggtcgc
     2401 cggattgtgg tcgttgatga cggtgagacg accgatgatc tggtgcgcga catgatcgaa
     2461 gtgctcacca gcatgtgcgc ccgcttgtat ggtc
//
LOCUS       DASWBF010000121        18993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_408245,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000121 DASWBF010000000
VERSION     DASWBF010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18993
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408245"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VF874_04130"
     CDS             <1..512
                     /locus_tag="VF874_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9175306.1"
                     /translation="WYYAWTPGTGYLANLLPDGSAPLTHQIPSLGIIVLAEVWKTTPF
                     MALLLLAGLALVPEELMRAAQVDGAGAWKRLVKVTLPLIKPAILVALLFRTLDAFRIF
                     DNIYILTGGANNTGSVSILGYDNLFKAFNLGLGSAISVLIFLCVAVIAFIFIKLFGTS
                     APGADDGNR"
     gene            514..1350
                     /locus_tag="VF874_04135"
     CDS             514..1350
                     /locus_tag="VF874_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9175307.1"
                     /translation="MAERVGAGRKAWWVIVDVLVVVYALFPVLWILSLSLKPTSTVKD
                     GKLIPTEITVDNYRGIFRGDLFGPALRNSIGIGLITTVIAVVIGAMAAYAVARLAFPG
                     KRLLVGVALLIAMFPQISLVSPLFDISRALGLFNTWPGLIIPYITFALPLAIYTLSAF
                     FREIPWDLEKAAKMDGATPFQAFRKVIVPLAAPGIVTAAILVFIFAWNDLLLAISLTA
                     TTASITAPVAIANFPGSSQFVEPTGSIAAGAMVITVPIIVFVLIFQRRIVAGLTSGAV
                     KG"
     gene            1354..2529
                     /gene="ugpC"
                     /locus_tag="VF874_04140"
     CDS             1354..2529
                     /gene="ugpC"
                     /locus_tag="VF874_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX9175308.1"
                     /translation="MAEIVLDRVAKSYPDGTTAVADLSLQIADGEFIILVGPSGCGKS
                     TTLNMIAGLEEITSGELRIDGQRVNEKAPKDRDIAMVFQSYALYPHMTVRQNIAFPLT
                     LAKMKRAEIASKVEETAKILDLTEHLDRKPASLSGGQRQRVAMGRAIVRNPKAFLMDE
                     PLSNLDAKLRVQMRSEISRLQRRLRTTTVYVTHDQTEAMTLGDRVVVLLAGVAQQIGA
                     PQELYDKPVNLFVAGFIGSPSMNFFPAQLTGVGVSLPFGEITLTQEVLDVVAQHPKPN
                     NVIVGVRPEHLEDANLIDTYQRLRALIFEVGVDIVESLGAEKIVHFTIEGASAHSAQL
                     AELAADSGVGENDFVARLPAESSAAAGERIELAFDTSKLAIFDADSGANLTIPRSTE"
     gene            2526..3062
                     /locus_tag="VF874_04145"
     CDS             2526..3062
                     /locus_tag="VF874_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEX9175309.1"
                     /translation="MTDVAADVRAHLRADFARAGEPDAASVTFLGVEPVDVLRFGPDT
                     HGVVHYASVGGSRHADGNPAEVVISLRSGANLPGLARSVAVVAAAPAVEGLVLTPDAL
                     VDLGQPLWQGAPFTAVLLADSVIDDMPPVVFLSATPITPTEAPWVRLKGVDAMRQAWQ
                     QDGVDVLDPARKASSPAG"
     gene            complement(3059..4165)
                     /gene="corA"
                     /locus_tag="VF874_04150"
     CDS             complement(3059..4165)
                     /gene="corA"
                     /locus_tag="VF874_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX9175310.1"
                     /translation="MPSFRALPPSLRPVVRAKASAPDARSIHVPVARATVDCGIYLDG
                     QRLPGKYTHAGALKKVHELEKAGNEAFVWIGLHEPDEHQMQAVADVFGLHELAVEDAV
                     HAHQRPKLERYDETLFMVLKTVKYVEHESMTKAREIVETGEIMIFIGRDFVVTVRHGD
                     FSGLKGLRSQLETSPSSTLKLGPYAVMHAIADHVVDHYLEVTDMLEVDVDAMEVEIFS
                     PRARTNIESIYLLKREVAELRRSVSPLTVALQRLATDHNDLVSIEVRRYMRDVLDHNI
                     KASDRIVSYDDMLSSLVQAALGKVAMQQNVDMRKISAWVAIAAVPTAIAGIYGMNFEH
                     MPELKATWGYPAVLTLIVVICLTLHHIFRKNKWL"
     gene            4391..5380
                     /locus_tag="VF874_04155"
     CDS             4391..5380
                     /locus_tag="VF874_04155"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX9175311.1"
                     /translation="MSTTPLKVAVTGAAGQIGYSLLFRLASGELLGADRPIELRLLEI
                     EPALKALEGVVMELDDCAFPLLAGVQIGADPNKIFDGANLALLVGARPRGPGMERSDL
                     LEANGAIFTAQGKALNEVAAEDIRIGVTGNPANTNALIAMSNAPDIPRERFSALTRLD
                     HNRAISQLAKKTGVAVTDIKKMTIWGNHSTTQYPDVFHAEIGGKNAAEVVNDPDWIEN
                     DFIPTVAKRGAAVIAARGASSAASAASATINAARDWLLGTPDGDWVSMAVTSDGSYDV
                     PEGLLSSFPVTTKDGNWTIVQGLDIDEFSRARIDKSTAELADERKAVTELGLI"
     gene            5413..6153
                     /locus_tag="VF874_04160"
                     /pseudo
     CDS             5413..6153
                     /locus_tag="VF874_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            6197..6994
                     /locus_tag="VF874_04165"
     CDS             6197..6994
                     /locus_tag="VF874_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9175312.1"
                     /translation="MAGCADRQPPRDVVVGAGADAESAVLAELYAAALRYRGSPARVE
                     RLPDPVAGLDSGEVSVVPGFTGRLLQRFSPGSKAIEDKDVYRAMVSALPEGIAAGDYA
                     MSAEDKPAVAVARTTADAWGGRDLAVLVRHCSQVTTGSVAGAQPPTRVGSCTLAAPRE
                     FPTEAELFDALRANQINAAWTTTASPDIPVDVVVLADRPPPMVRAENVVPLYRRNELS
                     EQEMLALNEVAGELDTSALADMRRHVAQGADPRQVADGWLAAHPLGR"
     gene            complement(6983..7822)
                     /locus_tag="VF874_04170"
     CDS             complement(6983..7822)
                     /locus_tag="VF874_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9175313.1"
                     /translation="MQGFAGKVAVVTGAGSGIGQALAIELARSGAKLAISDVEVDGLA
                     GTEERLKAIGAPVKTDRLDVTEREAFLAYADAVNDHFGTVNQIYNNAGIAFNGDVETS
                     QFKDIERVMDVDFWGVVNGTKAFLPHLIASGDGHVVNVSSVFGLFSVPGQAAYNSAKF
                     AVRGFTEALRQEMILARHPVKVTTVHPGGIKTNIVRNMTAVEGFDKDEMVQTFDKRLA
                     NTSPQKAARIILDGVRKNKARVLVGPDAKVLDIIVRLTGSGYQRLFSAVTTRAMPSQR
                     SST"
     gene            7933..8343
                     /locus_tag="VF874_04175"
     CDS             7933..8343
                     /locus_tag="VF874_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9175314.1"
                     /translation="MTNKFAAAVGSVLVSGGLVAAAMGLAAPAQADTTTTTDTFVDAL
                     TSAGLDNVVSGDAVALGQSICPMLADTGQNTADVASKVADVGGMSLGPATMFTGIAIS
                     MWCPGAIARIGNGQIDGIPGLDSLPALNGLIPGL"
     gene            complement(8435..12115)
                     /locus_tag="VF874_04180"
     CDS             complement(8435..12115)
                     /locus_tag="VF874_04180"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX9175315.1"
                     /translation="MSSSPSPFGQNEWLVEEMYRKFRDDPSSVDPSWHEFLVDYSPEP
                     TGNGQQTETTQKTEKTQEPQPKPAETIDKPPADGQAKAEPKQEAKPAAKAPVSVAPDD
                     EVQVLRGAAAAVVKNMSASLDVPTATSVRAVPAKLMIDNRTVINNHLKRTRGGKISFT
                     HVLGYAIVAAVKKFPNMNRHYAEVDGKPNVITPAHTNLGLAIDLHGKDGNRQLVVAGI
                     KKADTMRFGQFITAYEDIVRRARDGKLTAEDFGGVTISLTNPGTIGTVHSVPRLMRGQ
                     GAIIGVGAMEYPAEFQGASEERIAELGVGKLVTLTSTYDHRIIQGAESGDFLRTVHQL
                     LLSDDFWDDIFHELGIPYEPVRWRTDNPDPIVDKNARVIELIAAYRNRGHLMADIDPL
                     RLDKTRFRSHPDLDIATHGLTLWDLDRVFKVGGFGGAEYKKLRDVLGLLRDAYCRHIG
                     IEYTHILEPEQQKWLQERIEVKHEKPIVAQQKYILSKLNAAEAFETFLQTKYVGQKRF
                     SLEGAETVIPVMDAAIDQAAEHALDEVVVGMPHRGRLNVLANIVGKPYSQIFSEFEGN
                     LNPSQAHGSGDVKYHLGAHGTYIQMFGDNDIEVSLTANPSHLEAVDPVLEGLVRAKQD
                     LIEKGDAEDGFTVMPLMLHGDAAFAGQGVVAETLNLALLRGYRTGGTIHIIVNNQIGF
                     TTSPSDAKSSEYCSDVAKMIGAPIFHVNGDDPEASEWVARLAVDFRQKFKKDVVIDMI
                     CYRRRGHNEGDDPSMTQPAMYDVIDTKRGVRKTYTEALIGRGDISIKEAEDALRDYQG
                     PLEQVFNEVRELEKHEIEPSESVEAEQQLPRGLSTAVEKSLLARIGDAHLAFPEGFTV
                     HPRVKTGLEKRREMAYNGKVDWAFGELLALGTLVAEGRTVRLTGQDSRRGTFSQRHSV
                     IIDRKTGKEFTPLQVLATDEDGNPTGGRFMVYDSPLSEYAAVGFEYGYSLGDPDALVL
                     WEAQFGDFISGAQSVIDEFISSGEAKWGQTSDVVVLLPHGHEGQGPDHTSGRIERFLQ
                     VCAEGSMTVAQPSTPANYFHLLRRHALDNIHRPLIVFTPKSMLRNKAAVSDIREFTEM
                     KFRSVLEEPTYEFGDGDRSKVKRMLLVSGKLYYELVARKAKDKRDDIAIVRVEQLYPI
                     PRYRLPNTLDQYPNVDEYFWVQEEPANQGAWPTFGLALPELVPELKDIKRISRRAMSA
                     PSSGSAKVHAVEQQEILDEAFA"
     gene            complement(12310..13062)
                     /locus_tag="VF874_04185"
     CDS             complement(12310..13062)
                     /locus_tag="VF874_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175316.1"
                     /translation="MNAARRGPTALTTSTADDNPGAAARALSHILERGARVQGPAVEA
                     YVQRLRHANPGASPAEIVTKLEKRYLAAVMASGAAVGSAAAFPGIGTIAALSAVAGET
                     VVFLEATALFVLAVAEVHGIPAGHREHRRALVLAVLVGEDSKRPVRDLVGPGRLGGEW
                     LGESVATLELPIVSQLNNRLLRYFAKRYALKRGTIAFGKMLPMGIGAVIGGGGNRLMG
                     KKIISNARKAFGPAPSRWPVALHVLPPASASQ"
     gene            13197..14468
                     /locus_tag="VF874_04190"
     CDS             13197..14468
                     /locus_tag="VF874_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX9175317.1"
                     /translation="MATSRAVDVTSEPELTAEGSPVSRTRMILVLGALVALGPLTLDI
                     YLPALPRITAELSVSSSVTQLTLTGTLAGLALGQLIVGPLSDSLGRRKPLMAGIVLHM
                     LASLVCLMAPNIEVLGIARVLQGVGASAAMVVAIAVVGDLFDDSAAAVVLSRLMLVLG
                     VAPVVAPSLGAAVLLHASWHWVFAVLVVLAGGLFLVAAFALPETLPVAHRRPLRLGAV
                     GSTYLALLRDVRFVILVLVAALAMSGLFAYISGASFVLQGRYGLTQQVFAIVFGAGAV
                     SFITASQVNVVLLKRFSPQNIVVAALCFGVVAGAAFVGLASAHVGGLAAFLVPVWSIL
                     ASMGLVIPNAPALALSRHHEAAGTAAALLGAAQFGIGAAVSPVVGLLGNDELALAVVM
                     TTGVVVALLALVLVISRGTSGLRGRTDAAPA"
     gene            14628..16550
                     /locus_tag="VF874_04195"
     CDS             14628..16550
                     /locus_tag="VF874_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9175318.1"
                     /translation="MMVGFFMILVDSTIVAVANPRIMEKLNTDYDAVIWVTSAYLLAY
                     AVPLLVSGRLGDRFGPKNLYLIGLAIFTAASLWCGLAGSVEMLIAARVVQGLGAALLT
                     PQTLSTITRIFPPERRGVAMSLWGATAGVATLVGPLAGGALVDSLGWQWIFFVNVPIG
                     VVGIALAVWLIPVLPTHRHRFDVLGVLLSGIAMFLVVFGLQEGQSHNWAPWIWGTIAG
                     GVAMMSVFVYWQSVNKHEPLIPLRVFRDRDFSLSNAGIAVMGFVAVGMILPLMFYLQG
                     VCGLSPTRSALITAPMAVATGLLAPVVGHIVDRSHPRPVVGFGFAVMSIALTWLSFEM
                     TPTTPIWRLLLPFAAMGVGMAFIWAPLAVTATRNLPPELAGAGSGVYNATRQVGSVLG
                     SASMAALMTWRISDELPPMPQGTPRGGEGAVNELPAFLHQPFSDAMSISMLLPAFVGL
                     FGVVSALFLVGGRPSSSVANLVESQQAYVDDSEPPTDELPAVPVLADSYGGYVDDHDY
                     AEYSLAPPEPDSSPIPTDERLREEATATPADDSITEPLAVPSDRVRSEPIQLGPDNPN
                     ETLYHEPPEHWLRSTPEPIGFAHDGLHVKHEDTFREVGQEAPSRSRHASDSSPKFTWF
                     DPDADSYGRHAMPASD"
     gene            complement(16547..17893)
                     /locus_tag="VF874_04200"
     CDS             complement(16547..17893)
                     /locus_tag="VF874_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9175319.1"
                     /translation="MIPQLAAVVGGSHVSTDPDVLAGRSVDHTGRYRGHAAALVRPGS
                     ADEIAVVLAECRDAGAHVTIQGGRTSLVAGTVPEHDDVLLSTERLTSIGDVDTGERRV
                     RLGAGVTLAAAQRTAVSAGLLFGVDLAARDSATVGGMASTNAGGLRTVRYGNMGEQVV
                     GLEVALPDGSIVRRHSAVRRDNTGYDLTSLFVGAEGTLGVITELDVRLHSTPAHRVTA
                     VAGFADLSDVAAAGQEFRDLDGIAALELIDARALELTAAHLDIVAPVDAPWLLLVEMA
                     GDYDQSDRLADALAAAPLSGEPAVGVDAAAQQRLWRLRESIAEILGIFGPPLKFDVSL
                     PLPAIATFARQSAGLVAAHAPDAIPVLFGHIGEGNLHLNVLRCNVTGDRERALYSAMM
                     DLIAGCGGNVSSEHGVGSRKRGYIGMSREAADIAAMRTIKQALDPTGYLNAAVLFD"
     gene            complement(17890..>18993)
                     /locus_tag="VF874_04205"
     CDS             complement(17890..>18993)
                     /locus_tag="VF874_04205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017351370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9175320.1"
                     /translation="TGKTYSAARVIARLVNEHGWRVGVVAQSHAVIENLLDEIVGAGV
                     DPSVVAKKPGGHYTNPPAWRVISENRYADFIADNAGCVIGGTSWDFANAGRIPPGSLD
                     VLAIDEAGQFSLASTIAVSRAARNLLLLGDPQQLPEVSTGTHPEPVDSSALEWLLDGR
                     GVLPDEFGYFLEHTYRMHPAVCQAVSALSYENRLLPDAKTANRVLTGYPPGVHTICVD
                     HVDNDTSSSEEAVAIVDEIRRILGAQWTDEEESRPLGEDDVLVVAPYNAQVLLLRDQL
                     DAAGLSDVAVGTVDKFQGRQAAVVFVSMTASSIDDVPRGISFLLNRNRLNVAVSRAKY
                     SSVIVRSPLLTEYLPSTPDGLVDLGAFLSLSPC"
BASE COUNT         2940 a         6286 c         6324 g         3443 t
ORIGIN      
        1 gctggtacta cgcgtggaca ccgggcaccg gctatctggc caacctgctg ccggacggca
       61 gcgcgccgct gacacatcag atcccgtcgc tgggcatcat cgtgctcgcc gaggtgtgga
      121 agaccacgcc gttcatggcg ctgttgctgc tcgccggtct ggcgctggta cccgaagagc
      181 tgatgcgggc ggcccaggtg gacggcgcgg gggcctggaa gcggctcgtc aaagtcacgc
      241 tgccgctgat caagcccgcg atcctggtgg cgctgctgtt ccgcaccctc gatgcgttcc
      301 gcatcttcga caacatctac atcctcaccg gcggcgctaa caacaccggc tcggtgtcaa
      361 tcctgggcta cgacaacctg ttcaaggcct tcaacctcgg gctgggctcg gcgatcagcg
      421 tgctgatctt cttgtgtgtc gcggtgatcg cgttcatctt catcaagctg ttcgggacgt
      481 cggcgcccgg agcggacgac gggaatcgct gagatggcag aacgagtcgg cgccgggcga
      541 aaagcgtggt gggtgatcgt cgacgtcctg gtggtggtct acgcgctgtt tccggtgctg
      601 tggatcctgt cgctgtcgct gaagcccacg tctaccgtca aggacggcaa gctgattcca
      661 accgagatca cggtcgacaa ctatcggggc atcttccgtg gcgacttgtt cggcccggcg
      721 ctgcgcaatt cgatcggtat cgggctcatc accacggtga tcgcggtggt gatcggcgcg
      781 atggccgcct atgccgttgc gcgactggcg tttccgggca aacggctgct ggtcggtgtg
      841 gcgctgctga tcgcgatgtt cccccagata tcgctggtgt caccgttgtt cgacatctcg
      901 cgggcactcg gcctgttcaa cacctggccg ggcttgatca tcccgtacat cacattcgct
      961 ctgccactgg ccatctacac gctttccgcg ttcttccggg agatcccctg ggacctggag
     1021 aaggcggcca agatggacgg cgccacgccg tttcaggcgt ttcgcaaggt gatcgttccg
     1081 ctggccgcgc cgggcatcgt caccgcggcg atcctggtgt tcatcttcgc ctggaacgac
     1141 ctgctgctgg cgatctcgct gactgccacc acggcgtcga tcactgcgcc ggtggcgatc
     1201 gcgaattttc cgggaagttc tcagttcgtc gagcccaccg gatccatcgc ggccggcgcg
     1261 atggtgatta cggttccgat cattgttttt gttctaatct tccaacgacg gatcgtcgcc
     1321 gggctgacct ccggcgcagt gaaggggtag tcgatggccg agattgtgtt ggacagggtg
     1381 gcgaagagtt accccgacgg cacgacggcc gtggccgacc tgtcgctcca gatcgccgac
     1441 ggcgagttca tcatcctggt cggcccgtcg ggctgcggaa aatccacgac gctcaacatg
     1501 atcgccggac tcgaggagat cacgtcgggg gagttgcgca tcgacggtca gcgggtcaac
     1561 gagaaagcgc cgaaagaccg cgacatcgcg atggtcttcc agtcgtacgc gctctacccg
     1621 cacatgacgg tgcgccagaa catcgcgttc ccattgactt tggccaagat gaagagggcc
     1681 gagatcgcct cgaaggtcga agaaaccgcg aaaatccttg atctgaccga gcatttggac
     1741 cgcaagcctg ccagtctgtc cggcggtcag cggcagcggg tcgcgatggg ccgcgcgatc
     1801 gtgcgcaatc caaaggcgtt cctgatggac gagccgctgt ccaacctcga cgccaagctg
     1861 cgagtgcaga tgcgcagcga aatctctcgg ctgcagcggc gactgcgcac caccaccgtg
     1921 tatgtgacgc acgaccagac cgaggcgatg acgctgggcg atcgtgtcgt ggtgttgctc
     1981 gccggcgtcg cgcaacaaat cggcgcccca caagagcttt acgacaagcc ggtaaacctg
     2041 ttcgtggccg ggttcatcgg atcgccgtcg atgaacttct ttccggcgca gctgaccggc
     2101 gtgggagtca gcctgccgtt cggtgagatc acgctgaccc aggaagtgct cgacgtcgtt
     2161 gcgcaacacc cgaagcccaa caacgtgatc gtcggagttc ggccggaaca cctcgaagac
     2221 gcgaacctga tcgacacgta ccagcggctg cgcgcgctga tcttcgaggt cggtgtcgac
     2281 atcgtcgagt cgctgggcgc cgagaagatc gtgcacttca ctatcgaggg tgcgtcggcg
     2341 cactcggctc agctcgccga gttggccgcc gactccggag tcggcgaaaa cgatttcgtc
     2401 gcaaggcttc ccgcggagtc gagcgcagcc gccggcgagc ggatcgaatt agctttcgac
     2461 acatcgaaac tcgcgatctt cgacgccgat tccggtgcga atttgacgat tccacgctcc
     2521 accgagtgac ggacgtcgcc gccgacgttc gcgctcatct gcgggcggac ttcgcgcgcg
     2581 ccggcgagcc cgacgccgcc agtgtcacgt ttctcggcgt ggagccggtt gacgtgcttc
     2641 gcttcggccc tgacacacac ggcgtcgtcc attacgcgtc agtcggcggc tcgcggcacg
     2701 ccgatggcaa cccggccgag gtggtgatca gcctgcgaag cggcgcgaac ctgcccggct
     2761 tggcgcgcag cgtggccgtg gtggccgccg ccccggccgt cgaggggctg gtactgaccc
     2821 cggacgcgct cgtcgacctc ggccagccgc tctggcaagg cgcgccgttc accgcagttc
     2881 tgttggcaga cagcgtaatt gacgacatgc cacccgtggt gttcctgagt gcgaccccga
     2941 tcacgccgac cgaggcgccg tgggtgcggc tcaagggcgt cgacgcgatg cggcaggctt
     3001 ggcagcagga cggggtcgac gttctcgacc cggcgcggaa agcgagttcg cctgcgggct
     3061 agagccactt gttcttgcgg aagatgtgat gcagggtcag gcagatgacc actatcaggg
     3121 tcagcaccgc cggataaccc cacgtcgcct tcaactccgg catgtgttcg aagttcatcc
     3181 cgtagatgcc cgcgattgcc gtcggcaccg cggcgatcgc cacccacgcc gagatcttgc
     3241 gcatgtcgac gttctgctgc atcgcgacct tcccgagcgc cgcctgcacc agcgagctga
     3301 gcatgtcgtc gtagctgacg atccggtcgg aggccttgat gttgtggtcg agcacgtcgc
     3361 gcatgtaccg ccggacctcg atcgagacga ggtcgttgtg gtcggtggcc agccgctgca
     3421 gcgcgaccgt cagcgggctc actgaccggc gcagctcggc gacctcgcgc ttgagcaggt
     3481 agatgctttc gatattggtc cgggcgcgcg gcgagaagat ttcgacctcc atcgcgtcga
     3541 cgtcgacttc gagcatgtcg gtgacctcga gatagtggtc gacgacatgg tcggcgatcg
     3601 cgtgcatcac cgcgtacggg cccagcttca atgtcgacga cggcgacgtc tccagttggc
     3661 tgcgcaggcc cttgagccca ctgaagtcgc cgtgccggac ggtgaccacg aaatcgcgtc
     3721 cgatgaagat catgatctcg ccggtctcga cgatctcgcg ggccttggtc atcgactcgt
     3781 gctcgacgta cttcaccgtc ttcagcacca tgaacagcgt ctcgtcgtag cgctcgagct
     3841 tcggccgctg atgcgcatga actgcgtctt cgacggcgag ctcgtgcaag ccgaacacat
     3901 ctgcgaccgc ctgcatctgg tgttcgtcgg gctcgtgcag cccgatccag acgaacgcct
     3961 cattgcccgc cttctcgagt tcgtgcacct tcttcagcgc gccggcgtgc gtgtacttgc
     4021 ccggcaatcg ctggccgtcg agatagatcc cgcagtcgac cgtggcgcgg gcgaccggga
     4081 cgtgaatgga ccgtgcgtcc ggcgcactgg ccttggcccg cacaactggt cgaagtgatg
     4141 gaggcaacgc gcgaaacgat ggcatcggga cacctcccgt cgcgcgcagg tatggtccag
     4201 cgtcgctcaa tgctacgcgc cccgcctccc catttccccg cgagcgtgca tgttcgtacc
     4261 ccgacaccaa cgccggtgcg gagcctgcaa acctgcacgc tcgtgggcca gacgtagccg
     4321 aagattcgag gccgacggcg gtgcgctagt ttcgtcccgg acccaacccc ccgacgagga
     4381 gcaacctgac gtgagcacaa ctcccctcaa ggtcgccgtc accggcgccg ccggccagat
     4441 cggctacagc ctgctgttcc gcctggccag cggcgagctg ctgggggccg accgcccgat
     4501 cgagctgcgt ctgctcgaga tcgagcccgc actgaaggcc ctcgaaggtg tcgtgatgga
     4561 gctcgacgat tgcgccttcc cgctcctggc tggcgtgcag atcggcgcgg acccgaacaa
     4621 gattttcgac ggcgcgaacc tggccctgct cgtgggtgcc cgcccgcgcg gaccgggcat
     4681 ggagcgcagc gacctgctgg aggccaatgg cgcgatcttc accgcacagg gcaaggcgct
     4741 gaacgaggtt gcggccgagg acatccgcat cggcgtcacc ggcaacccgg ccaacaccaa
     4801 cgcgctgatc gcgatgagca atgcgcccga catcccgcgc gagcggtttt cggcgctgac
     4861 ccgcctcgac cacaaccgcg cgatcagtca gctggccaag aagactggcg tcgcggtcac
     4921 cgacatcaag aagatgacga tctggggcaa tcactccacc acccagtacc ccgacgtgtt
     4981 ccacgccgag atcggtggca agaacgcggc cgaggtcgtc aacgacccgg actggatcga
     5041 gaatgacttc atcccgacag tcgccaagcg cggagcggcc gttatcgccg cgcgcggcgc
     5101 ctcctcggcc gcgtcggccg cgtcggcgac catcaacgcc gcccgcgact ggctgctcgg
     5161 cactcccgac ggcgactggg tgtcgatggc ggttacctcc gacggcagct acgacgtgcc
     5221 cgagggcctg ctctcgtcgt tccccgtgac caccaaggac ggcaactgga cgatcgttca
     5281 gggcctcgac atcgacgagt tctcccgtgc ccggatcgac aagtcgactg ccgagcttgc
     5341 cgacgagcgc aaggcggtca ccgagctcgg cctgatctga acgcgcagta ccctgaggcc
     5401 gtgtccaaac acatgccgca aacagtgaaa ggtccgccag cgaacaccca gccgatcgtc
     5461 ctctccgacg acgagctctt cgaggcccac gtaggcggca agctttccgt tgagctgaag
     5521 gccccgctgg acactcagcg cgccctgtcg atcgcgtaca ccccgggcgt cgcgcaggtc
     5581 agtcgcgcga tcgccgccga ccacacgctg gccgcccgct acacctgggc gcatcggctg
     5641 gtagccgtgg tcagcgacgg cagcgcggtg ctcgggctcg gtgacatcgg cgcagccgca
     5701 gcgcttccgg tgatggaggg caagagcgcg ttgttcaagg cattcggcgg gctggactcg
     5761 atcccgatcg tgttggacac caaggacccc gacgagatcg tcgaaaccct ggtgcggctg
     5821 cggcccacat tcggcgccgt cagcctcgaa gacatctctg cgccgcgcta tttcgagatc
     5881 gaacggcgtg tcatcgaggc gctggactgc ccggtcatgc acgacgatca gcacggcacc
     5941 gcgatggtcg tgctcgcggc gatgctgggc gccaccaagc tgctcgaccg cgacatggcg
     6001 tcgcttcggg tggtgatctc cggtaccgga gccaccggcg tggcatgcgc gaatctgctt
     6061 ctcgccatgg ggatttcaga catcatcgtc ctcgattcgc acggcattct tcattccagc
     6121 cgcgacgaca tgaacggcgt caaggccgag cttgactaga cgccttctcg gcgcgctgct
     6181 ggcggtcgcg ctgctgatgg ccggctgcgc cgatcggcaa ccaccgcgcg acgtggtggt
     6241 cggagccggc gccgacgccg aatcggcggt gctcgccgag ttgtacgccg cggcgttgcg
     6301 ctaccgcgga tcgccggcac gcgtcgagcg tctgccagac cctgtcgcag ggcttgacag
     6361 cggagaggtc agtgtggtgc ccgggttcac gggtcggctg ctgcagcgat tctcgcccgg
     6421 cagcaaggca atcgaggaca aggacgtcta ccgcgcgatg gtgtccgcgc tgcccgaagg
     6481 catcgccgcg ggggattacg cgatgtcggc cgaggacaag cctgccgtgg ccgtggcgag
     6541 aacgaccgct gacgcatggg gcggacggga cttggcggtg ctggtgcgcc actgctcaca
     6601 ggtgacgacg gggtcggtgg ccggcgcgca accgccgacg cgggtcggta gttgcacact
     6661 ggccgcccca cgcgaatttc ccacggaggc agaacttttc gacgcgctga gggccaacca
     6721 gatcaacgcc gcgtggacca ccaccgcgtc acccgacata cccgtcgacg tggtggtgct
     6781 cgccgaccgt ccgccgccga tggtgcgggc cgagaacgtg gtgccgctgt accgccgaaa
     6841 cgaactgtcc gagcaggaaa tgctggccct caacgaggtt gccggggagc tcgacaccag
     6901 cgcgctggcc gacatgcgtc gccacgtggc gcagggggcc gatccgcggc aggtggccga
     6961 cggatggctg gcggcccacc cgctaggtcg atgagcgctg cgacggcatc gcccgggtgg
     7021 tgaccgcgga gaacagccgc tggtagcccg aaccggtcag ccgcacgatg atgtcgagca
     7081 ccttggcatc tgggcctacc agcacccgag ccttgttctt gcgcactccg tcgaggatga
     7141 tccgcgccgc cttctgcggt gacgtgttgg ccagcctctt gtcgaacgtc tgaaccattt
     7201 cgtccttgtc gaagccctcg accgcggtca tgttgcgcac gatgttggtc ttgatgccgc
     7261 cgggatgcac ggtggtcacc ttcaccgggt ggcgggcgag gatcatctcc tggcgcagcg
     7321 cctcggtgaa cccgcggacc gcgaacttcg ccgagttgta cgccgcctgg ccgggcaccg
     7381 agaacagccc gaagacgctg gagacgttga cgacgtgccc gtcgccggac gcgatcagat
     7441 gcggcaggaa ggccttggtg ccgttgacga cgccccagaa gtcgacatcc atcacccgct
     7501 cgatgtcctt gaactggctg gtctcgacgt cgccattgaa cgcgataccg gcgttgttgt
     7561 agatctggtt gaccgtgccg aagtggtcgt tgaccgcgtc ggcgtaggcc aggaacgcct
     7621 cgcgctcggt gacgtcgagg cggtcggtct tcactggcgc cccgatggcc ttgagccgct
     7681 cctcggtgcc ggccaagccg tcgacctcga cgtcgctgat cgccagcttg gcgcctgacc
     7741 gcgccaattc gatggccagc gcctggccga tccccgagcc ggctcccgtg acgacggcaa
     7801 ctttcccggc aaacccctgc atgatcaccc catccatccg cgagcttgtg gccgtcactt
     7861 tacgcacgct cgcgggaacg ctgagtccgc ggattcggta acgcgggcgg tccccgctac
     7921 gatccccaag tcatgacaaa caaatttgct gcggccgtcg ggtcggtgct ggtttccggg
     7981 ggtctcgtcg cagcggctat ggggctcgcc gccccggcgc aggccgacac gaccacgacc
     8041 accgacacct tcgtcgatgc gctgaccagc gccgggctgg acaacgtcgt ctcgggcgac
     8101 gccgtcgcgc tcggacagtc gatctgtccg atgctggccg atacgggtca gaacaccgcg
     8161 gatgtcgcct cgaaggtcgc cgatgtcggc ggcatgtcgc tgggccccgc gacgatgttc
     8221 accgggatcg cgatctcgat gtggtgccca ggcgccatcg cccggatcgg caacgggcag
     8281 atcgacggca taccggggct ggacagcttg ccggcgctca acggcctgat ccccggcctg
     8341 tagcccgcac gttcgttgcg cgcgagcgtg cgctgttgta cctgaaaaca tcggtgtgtc
     8401 ggggtacaga catgcacgct cgcgcaaaga agggctacgc gaacgcttcg tcgaggattt
     8461 cctgctgctc gacggcgtgc accttcgccg agccggatga cggcgccgac atcgcacgcc
     8521 gggagatccg tttgatgtcc ttgagctccg gtaccagctc gggcagcgcc agcccgaacg
     8581 tcggccatgc gccctggttg gccggctcct cctgcaccca gaagtactcg tcgacgttcg
     8641 gatactggtc gagcgtgttg ggcagccggt agcgggggat cggatacagc tgctcgaccc
     8701 gcacgatcgc gatgtcgtcg cgcttgtcct tggccttgcg cgcaaccagt tcgtagtaga
     8761 gcttgccgct aaccaacagc atcctcttga ccttgctgcg gtcgccgtcg ccgaactcgt
     8821 aggtaggctc ttcgagcacc gagcggaact tcatctcggt gaactcgcgg atgtcggaga
     8881 cggcggcctt gttgcgcagc atcgacttcg gcgtgaacac gatcagcggc cggtgaatat
     8941 tgtccagcgc gtggcggcgc agcagatgga aatagttggc cggcgtggac ggctgcgcca
     9001 cggtcatcga cccctcggcg cacacctgca ggaaccgctc gatgcggccc gacgtgtggt
     9061 cggggccctg gccctcgtgc ccgtgcggga gcagcaccac cacgtcggac gtctggcccc
     9121 acttcgcttc acctgagctg atgaactcgt ctatcaccga ctgcgcgccg ctgatgaagt
     9181 cgccgaactg cgcctcccac aacaccaggg cgtccgggtc gcccagcgag tagccgtatt
     9241 cgaagccgac cgcggcgtat tcggatagcg gcgagtcgta caccatgaac cggccgccgg
     9301 tggggttgcc gtcctcgtcg gtggccagca cctgcagcgg cgtgaactcc ttgcccgtct
     9361 tgcggtcgat gatcaccgaa tggcgctggc tgaacgtgcc gcggcggctg tcctgcccgg
     9421 tcaaccgtac ggtgcggccc tcggcgacca gcgtgccgag cgccagcaac tcaccgaatg
     9481 cccagtcgac cttgccgttg taggccatct cgcgacgctt ttccagcccg gtcttcaccc
     9541 gcgggtgaac cgtgaagccc tccgggaacg ccagatgcgc atcgccgatg cgggccagca
     9601 gcgacttctc gaccgccgtg gacaggcctc gcggcagctg ctgctccgcc tctacggact
     9661 cgctgggttc gatctcgtgc ttctccagtt cccgcacttc gttgaagacc tgctcgagtg
     9721 gcccctggta gtcgcgcaac gcgtcctcgg cctctttgat cgagatgtcg ccgcggccga
     9781 tcaacgcctc ggtgtacgtc ttgcgcacgc cgcgcttggt gtcgatgacg tcgtacatcg
     9841 ccggctgggt catcgacggg tcgtcgccct cgttgtgacc gcggcggcgg tagcagatca
     9901 tgtcgatgac gacgtccttc ttgaacttct gccggaagtc cacggccagc cgcgccaccc
     9961 actcgctggc ttctggatcg tcgccgttga cgtggaagat cggcgcgccg atcatcttcg
    10021 cgacgtcgct gcagtactcc gacgacttcg cgtcgctcgg cgatgtggtg aagccgatct
    10081 ggttgttgac gatgatgtgg atcgtgccgc cggtgcggta accgcgcagc agcgccaggt
    10141 tcagcgtctc ggcgaccacg ccctgcccgg cgaatgcggc gtcgccatgc agcatcaatg
    10201 gcatgacggt gaacccgtcc tccgcatcgc ccttttcgat caggtcctgc tttgcccgca
    10261 ccagcccctc gaggacgggg tcgacggcct ccaggtgaga cgggttggcg gtcagcgaga
    10321 cctcgatgtc gttgtcgccg aacatctgga tgtacgtgcc gtgggcgccg aggtggtact
    10381 tcacatcacc cgagccgtgc gcctgcgacg ggttcaagtt gccttcgaac tcgctgaaga
    10441 tctgcgagta cggcttgccg acgatgttcg ccagcacgtt gagccgtccg cggtgcggca
    10501 tcccgaccac cacttcgtcg agcgcgtgtt cggcggcctg gtcgatcgcc gcgtccatca
    10561 ccggaatgac ggtctcggcg ccttcgagcg aaaaacgttt ctgcccaacg tatttggtct
    10621 gcaggaaggt ctcgaacgcc tcggccgcgt tgagcttgga gaggatgtac ttctgctgag
    10681 cgacgatcgg cttctcgtgc ttgacctcga tgcgttcctg cagccacttc tgctgctcgg
    10741 gctcgaggat gtgggtgtat tcgatgccga tgtggcggca gtacgcgtcg cgcagcaggc
    10801 ctagcacgtc gcgcaacttc ttgtactcgg cgccgccgaa gccgccgacc ttgaacaccc
    10861 ggtccaggtc ccacagcgtc agaccgtgcg tcgcgatgtc gagatcgggg tggctgcgga
    10921 accgcgtctt gtccaggcgc agcgggtcga tgtcggccat caggtggccg cggttgcggt
    10981 aggccgcgat cagctcgatg acgcgggcgt tcttgtcgac gatcgggtcc gggttgtcgg
    11041 tgcgccaccg gaccggctcg tacgggatgc cgagctcgtg gaagatgtcg tcccagaaat
    11101 cgtcggagag cagcagctgg tggaccgtgc gcaggaagtc gccggactcc gcgccctgga
    11161 tgatgcggtg gtcgtaggtc gacgtcaacg tcaccagctt gccgacgccc agctccgcga
    11221 tccgctcctc gctggcaccc tggaactcgg cggggtattc catcgcgcca acgccgatga
    11281 tcgcgccctg gccgcgcatc agccgcggca ccgagtgcac ggtgccgatg gtgccggggt
    11341 tggtcaacga gatcgtcaca ccgccgaagt cctcggccgt cagcttgccg tcgcgggccc
    11401 gccgcacgat gtcctcgtat gcggtgatga attggccgaa ccgcatggtg tcggccttct
    11461 tgatgccggc gacgacgagt tggcggttgc cgtccttgcc atgcaggtcg atggccaggc
    11521 ccaggttggt gtgggccggc gtgatgacgt tcggcttgcc gtcgacttcg gcgtagtggc
    11581 ggttcatgtt cgggaacttc ttgaccgcag ccacgatcgc gtatccgagc acgtgcgtga
    11641 acgagatctt gccgccgcgt gtccgcttga ggtggttgtt gatgacggtg cggttgtcga
    11701 tcatcaactt agccggaacg gcgcgcacgc tggtggccgt cggcacgtcc agcgacgccg
    11761 acatgttctt gaccaccgcg gccgcggcgc cgcgaagcac ctgcacctcg tcgtcgggtg
    11821 ccacactcac gggtgccttc gcggccggct ttgcctcctg cttcggctcg gccttcgcct
    11881 gcccgtcggc tggcggcttg tcgatggttt cggccggttt cggctgcggc tcttgggtct
    11941 tctccgtctt ctgcgtcgtc tcggtctgct ggccgtttcc ggtcggttcg ggtgagtagt
    12001 cgacgaggaa ctcgtgccag ctcgggtcga ccgacgaggg atcgtcgcgg aacttgcggt
    12061 acatctcttc gacgagccac tcgttttgcc cgaatggcga aggtgaactg ctcacagcgg
    12121 ctgctcgcct cgattcctct gtcggggaag cgtcgttacg gcgctcgccc acctgtgtta
    12181 catggggacg gtctctgctc tttccgccgc cctaaaggct aacgctttgg tcatggccgc
    12241 gaccatgcca gcggcattcc cgaccgcacc gtttctaaca gtacgcccgt tatgggaaac
    12301 caaggtgcgt tattgagacg cgctcgctgg cggcagcaca tgcaacgcga cgggccagcg
    12361 acttggggcg ggcccaaaag ccttgcgggc gttgctgatg atcttcttgc ccatcaaacg
    12421 gttgccgccg ccgccgatca ccgcgccgat gcccatcggc agcatcttgc cgaacgcgat
    12481 cgtgccgcgc ttgagcgcgt agcgcttcgc aaagtacctg agcagccggt tattgagttg
    12541 cgaaactatc ggcagttcca gtgtggccac cgactcgccc agccactcac cgccgaggcg
    12601 gccgggaccg accaagtcgc ggaccggccg cttactgtct tcgccgacca gcacggccag
    12661 caccagtgcg cggcggtgct ctcggtgtcc ggccggaatc ccgtgcactt cggcgaccgc
    12721 cagcacgaat agtgcggtgg cctccaggaa cacgacggtc tcgccggcga cggcagacag
    12781 cgccgcgatg gtgccgatgc ccgggaaagc cgcggctgaa ccgaccgcag ctccgctggc
    12841 catgaccgcc gccaggtacc gcttctcgag cttggtcacg atctcggccg gcgatgcacc
    12901 aggattcgcg tgtcgcaacc gttggacgta cgcctcgacc gcgggtcctt gcacacgggc
    12961 accgcgctcc aagatgtgcg acagcgcccg cgcggccgcg ccggggttgt cgtcggcggt
    13021 ggacgtcgtc agcgctgtcg gtccccggcg tgcggcgttc atcgggcctc ctccgctaat
    13081 cgcgtctctc ttcacagtac tgtcagcaac tcgacatctc ggccttgtga catggcgacg
    13141 acggcagtcc gtcgtcagcg ggaagaaagt atcgacgcgc ggcgctgctt accggcatgg
    13201 caacatcgcg ggctgtggat gtgacgagcg aacctgaact gacggcagag gggtcgccgg
    13261 tcagccgcac ccggatgatc cttgtgctcg gcgcactcgt cgcattgggg ccactcacgc
    13321 tggacatcta tctgccggcg ttgcccagga tcaccgccga gttgtcggtg tcgtcgtcgg
    13381 tgacgcagtt gacgttgacc ggcacactgg cggggctggc gttgggccag ctgatcgtcg
    13441 gaccgctgtc ggattcgcta ggccgccgca aaccgctgat ggccgggatc gtgctgcaca
    13501 tgttggcgtc actggtgtgc ctgatggccc ccaacatcga ggtgctcggc atcgccaggg
    13561 tgctgcaggg cgtaggcgcg tccgcggcga tggtggtggc gatcgccgtc gtcggtgacc
    13621 tgttcgatga ctcggccgcc gccgtcgtgc tgtcgcggct gatgctcgtg ctgggggtgg
    13681 caccggtcgt cgccccgtcg ctgggcgcgg cggtcttgct gcacgcgtca tggcattggg
    13741 tgttcgcggt gctcgtggtg ctggccggcg gcttgttcct cgtggccgcg ttcgcgctgc
    13801 ccgagacgct gcctgtcgcg caccgcaggc cgctgcggct gggtgccgtc ggatccacgt
    13861 acctcgcgct gctgcgtgat gtcagattcg tgatcttggt gctggtcgcc gcactcgcga
    13921 tgtcggggct gttcgcgtat atctccggcg cgtcgttcgt gctgcagggc cgctacgggc
    13981 tcacccagca ggtgttcgca atcgtgttcg gcgccggggc ggtgtcattc atcaccgcat
    14041 cgcaagtcaa tgtcgttctg ctgaaacggt tttcaccgca gaacatcgtg gtcgccgcgc
    14101 tgtgcttcgg cgtggtcgcc ggtgcggcgt tcgtcggcct cgcatcggcc catgtcggcg
    14161 ggctggcggc cttcctcgtt ccggtgtggt cgatcctggc gtcgatgggg ttggtgattc
    14221 ccaatgcgcc ggccttggcc ctgtcccgcc accacgaagc ggcgggcacg gctgctgcgc
    14281 tgctgggagc ggcgcagttc ggcatcggcg cggccgtgtc gccggtcgtc gggctgctcg
    14341 gcaacgacga actcgcactg gcggtggtga tgacgaccgg cgtggtggtc gcgctgttgg
    14401 cgctcgtgct ggtgatcagt cggggcacct ctggcttgcg ggggaggacc gacgccgcac
    14461 cggcgtagta ccgtctcacg gattactccg cgcggagtac tgggcgccgg aataggaacg
    14521 gaccgctgca tggcctatca ggtgacgtcg tgaccgcgac caccgagctg ccggccgacg
    14581 agcaggagac gcagccgcag gacaacccgt ggcgtgcgct gtgggccatg atggtcggct
    14641 tcttcatgat cctggtcgac tcgacgatcg tcgcggtcgc caatccgcgg atcatggaga
    14701 agctcaatac cgactatgac gcggtgatct gggtgaccag cgcgtacctg ctggcgtacg
    14761 cggtgccgct gctggtctcc ggccggctcg gtgaccggtt cggccccaag aacctgtacc
    14821 tgattgggtt ggcgatcttc acggccgcgt cgctgtggtg cggactggcc ggctccgtcg
    14881 aaatgctgat cgccgcccgc gtggtgcagg gcctgggcgc ggcgctgctg acgccgcaaa
    14941 cgctgtcgac gatcacgcgg atcttcccgc cggagcgccg gggcgtcgcg atgagcctgt
    15001 ggggcgcgac ggcgggggtg gcgacgttgg tcgggccact cgccggtggg gcgctggtcg
    15061 attcgctggg ctggcagtgg atcttcttcg tcaacgtgcc gatcggcgtg gtcggtatcg
    15121 cgctggccgt ctggctgatc ccggtgctgc cgacgcaccg gcatcgcttc gacgtgctcg
    15181 gcgtgctgct gtcgggcatt gcgatgttct tggtcgtgtt cggcctgcag gagggccaga
    15241 gtcacaactg ggcgccgtgg atctggggca ccatcgcggg cggcgtcgcg atgatgagcg
    15301 tcttcgtcta ctggcagtcg gtcaacaagc acgagccgct gatcccgctg cgggtgttcc
    15361 gcgatcgcga cttctcgctg tcgaacgccg ggatcgccgt gatgggcttc gtcgcggtgg
    15421 gcatgattct tccgctgatg ttctatctgc agggagtgtg cgggttgtcg cccacgcgct
    15481 cggcgctgat caccgcgccg atggcagtcg ccaccgggct actggcgccc gtcgtcggcc
    15541 atatcgtcga ccgatcccat ccccgcccgg tcgtcggctt cggcttcgcg gtgatgtcga
    15601 tcgcgttgac ctggttgtcc ttcgagatga cgcccacgac cccgatctgg cggctgctgc
    15661 tgccgttcgc cgcgatgggt gtcggcatgg cgttcatctg ggcgccgctg gcggtcaccg
    15721 caacccgcaa cctgccacca gaactggccg gcgccggatc gggggtctac aacgcgacgc
    15781 ggcaggtcgg ttcggtgctg ggcagcgcga gcatggccgc gttgatgacc tggcggatca
    15841 gcgacgaact tccgccgatg ccgcagggca ctccgcgcgg cggcgaaggt gcggtgaacg
    15901 aactacccgc gtttctgcac caaccgttct cggacgcgat gtccatctcc atgctgctgc
    15961 ccgcgttcgt cgggttgttc ggcgtcgtct cagccttgtt tctcgtcggc ggccggccgt
    16021 cgtctagcgt ggcgaacctc gttgagagtc aacaggctta cgtcgacgac tctgagccgc
    16081 ccaccgacga gttgccggct gtccccgtcc tcgccgacag ttatggcggc tacgtcgacg
    16141 accacgatta cgccgaatac agcctggcac cgcccgagcc ggacagttcg cctataccga
    16201 ccgatgagcg cttgcgcgaa gaggcgacgg caacgcccgc cgacgacagc atcaccgagc
    16261 ccctggccgt cccgtccgac cgggtacgga gcgagccaat tcagctgggt ccggacaatc
    16321 cgaacgagac gctgtaccac gagccgccgg agcattggct gcgctccacg ccggaaccga
    16381 ttggcttcgc gcacgacggc cttcatgtca agcacgaaga cacgtttcgc gaggtcggcc
    16441 aggaggcgcc gtcgcgaagc aggcacgcgt ccgacagttc accgaaattc acctggttcg
    16501 accctgatgc cgacagctac ggcaggcacg cgatgcccgc cagtgactag tcgaacaaca
    16561 cggcggcgtt caggtagccg gtggggtcca gcgcttgctt gattgtgcgc atcgcggcga
    16621 tgtcggctgc ctcgcgggac atgccgatgt agccgcgctt gcggctgccg acgccgtgct
    16681 ctgagctgac gttgccgccg cagcccgcga tcaggtccat catcgccgag tacagcgccc
    16741 gctcacggtc cccagtgacg ttgcagcgca acacgttcag gtgaagattg ccctcaccga
    16801 tgtgaccgaa cagcaccggg atcgcgtccg gcgcgtgcgc ggcgaccagg ccggctgact
    16861 ggcgtgcgaa tgtcgcaatc gccggcaacg gcagcgacac gtcgaacttc agcggcgggc
    16921 cgaagatgcc caggatctcg gcgatcgatt cgcggagccg ccaaagtcgt tgctgcgcag
    16981 ccgcatcaac gccgacggcc ggctcaccgg acagtggggc agcggctagt gcatcggcga
    17041 gtctgtcaga ctgatcgtag tcgccggcca tctcgacgag cagtagccac ggtgcgtcca
    17101 ccggtgcgac gatgtcgagg tgggccgcgg tgagttcgag ggcacgggcg tcgatcagct
    17161 ccagagcggc gatgccgtcg aggtcgcgga actcctgacc ggcggctgcg acgtcggaca
    17221 ggtcggcgaa cccggcgacc gcggtgactc ggtgcgccgg tgtggaatgc agccgcacgt
    17281 cgagctcggt gatcacgccc agcgtgccct ctgcgccgac gaacagcgag gtcagatcgt
    17341 aaccggtgtt gtcgcggcga acagcgctgt gccgccgcac aattgacccg tcgggcagcg
    17401 ccacctcgag cccgaccacc tgctcgccca tgttcccgta gcgcacggtc cgcagcccgc
    17461 ccgcgttcgt cgacgccatg ccgccgaccg tcgcggaatc gcgcgccgcc aagtcgacgc
    17521 cgaacagcag tccggcagac accgccgtgc gctgtgcggc agccaacgtc acgccggccc
    17581 cgagccgcac tcgccgctcg ccggtgtcga cgtctccgat cgaggtgagt cgctcagtgg
    17641 acagcagcac gtcgtcgtgt tcgggaaccg tgccggccac caacgacgtt cggccgccct
    17701 ggatcgtcac atgcgcgccc gcgtcgcggc actcggccag gaccacagcg atctcgtcgg
    17761 ccgaccccgg gcgcaccagc gcggcggcgt gaccgcggta gcggccggtg tggtcgacgc
    17821 tgcgcccggc gagcacgtcg ggatcggtgc tgacgtggct gccccccacg acggcggcca
    17881 gttgcggtat caacatggcg acaacgacag gaacgcgccg agatcgacca gtccgtcagg
    17941 tgtcgacggc agatactcgg tcagcagtgg cgaacgcacg atgacgctcg agtatttggc
    18001 gcggctgacc gctacgttga gccggtttcg gttgagcagg aaggaaattc cgcgcggtac
    18061 gtcatcaatt gatgacgccg tcatcgacac gaacaccacg gccgcctgtc tgccctggaa
    18121 cttgtcgacg gtgccgacgg caacgtcgga gaggcccgcc gcgtcgagct ggtcgcgcag
    18181 cagcagcacc tgtgcgttgt acggagcgac cacgagcacg tcgtcctcgc ccagcggacg
    18241 gctttcctct tcgtcggtcc actgcgcacc gagaatgcgt cggatctcgt cgacgattgc
    18301 cacggcctcc tcgctgctcg acgtgtcgtt gtcgacgtgg tccacgcaga tggtgtgaac
    18361 cccgggcggg tatccggtca gtacccggtt ggcggtcttt gcgtcgggaa gcagccggtt
    18421 ctcgtaggac agcgccgaaa ccgcttggca cacagccgga tgcatgcggt aagtgtgctc
    18481 gaggaaatac ccgaactcgt cgggcagcac gccgcggccg tcgagcagcc actccagcgc
    18541 cgagctgtcc accggttcgg ggtgggtgcc ggtgctgact tccggcagtt gctgcgggtc
    18601 gccgagcagc agcaggttac gcgctgcgcg ggataccgcg atggtgctcg cgagcgagaa
    18661 ctgcccggct tcgtcgatgg ccagcacgtc gaggctgcca ggcgggatgc gccccgcgtt
    18721 ggcgaagtcc cacgatgtgc cgccgatgac gcagcccgcg ttgtcggcga tgaagtcggc
    18781 gtaccggttt tcggaaatca ctcgccatgc tgggggattc gtgtagtgac cgccgggttt
    18841 tttcgcgacg accgacgggt cgacgccggc gcccacgatt tcgtcgagca ggttctcgat
    18901 caccgcgtgg gactgcgcca ccacgccgac gcgccagccg tgctcgttga ccagccgggc
    18961 gatcacccgc gcggcactat acgtcttgcc ggt
//
LOCUS       DASWBF010000122         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1197812,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000122 DASWBF010000000
VERSION     DASWBF010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197812"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VF874_04210"
     CDS             <1..1228
                     /locus_tag="VF874_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9175321.1"
                     /translation="AAGLLDVDVGEIVRPGILRIEDDRIVGVGGAAQAGFVGSADEGD
                     LIDLGEQILLPGLMDMEVNLLMGGRGETPALSQVQDDPPTRVLRAVGNARRTLRAGFT
                     TVRNLGLFVKTGGYLLDVALGKAIDAGWIEGPRIVPAGHAITPTGGHLDPTMFAAFAP
                     HVLELTVEEGIANGVDEIRRAVRYQIKHGAQLIKVCCSGGVMSLTGEPGAQHYSDEEL
                     RAIVDEAHRRGLRVAAHTHGAEAVKHAVAAGIDCIEHGFLIDDEAIATLVEHGTFLVT
                     TRRLAEAMDVSKAPKELQAKAAEMFPKARTSIKAAHEAGVKIAVGTDAPAIPHGRNAD
                     ELVTLVDWGMPAADVLRAATVVAADLINATDRGRLAEGQLADVIAVPGDPLSDITVTQ
                     KVSFVMKGGKVYANSN"
     gene            1212..1631
                     /locus_tag="VF874_04215"
     CDS             1212..1631
                     /locus_tag="VF874_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9175322.1"
                     /translation="MPTPTEDIVEIQQLLAKYAVTITQQDIDGLIEVFTPDGTYSAFG
                     ETYALERFPVLVDAAPKGLFLTGTALVGLDGDTATGTQPLCFIDHATHDMRIGYYRDT
                     YVRTADGWRLRTRAMTFIRRSGAHDSGRPHAVGSPTA"
     gene            1628..2770
                     /locus_tag="VF874_04220"
     CDS             1628..2770
                     /locus_tag="VF874_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9175323.1"
                     /translation="MNVKEFRAGLQAWLDENNLTPGQDHSLEGHMKQLARVHRALYDA
                     DWMRYGWPMEVGGLGGPAMLRAIVGEEVVGRGLAEPGPYSMVEVLAPTMIDYAPRELA
                     AEMVPRLLSGREQWCQGFSEPGSGSDLASLSTRATPRGDNWVVNGQKVWTSFAQYSQR
                     CILLTRTGPGHNGITAFFVDMDTPGITVRPLRTMHGVDEFCEVYFDDVVIPGDRMLGK
                     PGDGWQLAMDLLPHERSTCFWQRIAYLYARFDRLIAEADARSKIAEADARSKIADSDA
                     PGDSELGSAYLALHTLRCRSRATQHRLADGARLGPETSIDKVLLATAEQKLYDTVRDL
                     LPGAVELDDTEWRTEFLYSRAATIYGGTAEIQRNIIARRLLDLGKE"
     gene            2797..3276
                     /locus_tag="VF874_04225"
                     /pseudo
     CDS             2797..3276
                     /locus_tag="VF874_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781373.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
BASE COUNT          571 a         1061 c         1108 g          536 t
ORIGIN      
        1 ggccgccggg cttcttgacg tcgacgtggg cgagatcgtc cggccaggca tcctgcggat
       61 cgaggacgac cggatcgtcg gcgtcggggg tgcggctcag gctggttttg tcggctccgc
      121 cgacgagggt gacctcatcg acctcggtga gcagatcctg ctgccgggtt tgatggacat
      181 ggaggtcaac ctgctgatgg gcgggcgcgg ggaaacccct gcgctctccc aagttcagga
      241 cgatcctccg acacgcgtgc tgcgcgcggt cggcaacgcc cgtcgtacgc tgcgcgccgg
      301 cttcaccacc gtccggaact tgggtctctt cgtcaagacc ggcggctacc tattggacgt
      361 cgcgctcggc aaggcgatcg acgccggctg gatcgaaggt cctcggatcg tgcccgcggg
      421 gcacgcgatc acgcccacgg gcgggcatct cgacccgacg atgttcgccg cgttcgcgcc
      481 tcacgttctc gagctcaccg tcgaggaggg catcgccaac ggcgtcgacg agatccgcag
      541 ggcggtgcgg taccagatca agcacggcgc acagctgatc aaggtctgct gttctggtgg
      601 ggtgatgtca ctgacgggag agcctggggc gcagcactat tcggacgagg agttgcgagc
      661 tatcgtcgac gaggcgcacc gccgcggcct gcgggtcgcc gcgcacaccc acggcgccga
      721 ggcggtcaag cacgccgtgg cggccggtat cgactgcatc gagcacggtt tcctgatcga
      781 cgacgaggcg atcgcgacac tggtcgagca cggcacgttc ctggtcacca ccaggcggct
      841 ggccgaggca atggacgtct ccaaggcgcc aaaggagctg caagccaagg ccgccgagat
      901 gtttcccaag gcgcgcacat cgatcaaggc ggctcacgaa gccggcgtga agatcgcggt
      961 cggcaccgac gcccccgcaa ttccccacgg ccgcaacgcc gacgagctcg tgacgctggt
     1021 cgactggggt atgccggcag cggacgtgct tcgcgctgcg acggtcgtgg cggccgatct
     1081 gatcaacgcc accgatcgcg gccggctggc cgaggggcaa ctcgccgacg tcatcgcggt
     1141 gccgggagat ccgttgtcag acatcaccgt tacgcagaag gtaagcttcg tgatgaaggg
     1201 cggaaaggtc tatgccaact ccaactgagg acatcgtcga aatccagcag ctgctggcca
     1261 agtatgcggt gacgatcacc cagcaggaca tcgacgggct gatcgaagtg ttcacgcccg
     1321 acggtacata cagcgcgttc ggcgaaacgt atgcgctgga acggtttccg gtcctggtgg
     1381 atgccgcccc caagggtttg ttcctcaccg gcaccgcgtt ggtcggcctc gacggcgaca
     1441 ccgcgaccgg cacccagccg ttgtgcttca tcgaccacgc cacccacgac atgcgcatcg
     1501 gctactaccg cgacacctac gtgcggaccg ccgatggctg gcgactgcgg acacgggcga
     1561 tgacgttcat ccgccgcagc ggcgctcacg attcaggccg cccgcacgcg gtcggcagcc
     1621 ccactgcatg aatgtcaaag aatttcgggc cggcttgcaa gcgtggctcg acgagaacaa
     1681 cctgactcca ggccaggatc attcgctcga aggccacatg aagcagctgg cccgggttca
     1741 ccgcgcgctg tatgacgccg actggatgcg ctacggctgg cccatggagg tcggcggcct
     1801 aggcgggcct gcgatgctcc gcgcgatcgt cggggaagag gttgtcggcc gtggtcttgc
     1861 cgaaccaggt ccgtactcga tggtcgaggt gctcgcaccc acgatgatcg actacgcgcc
     1921 aagagaactc gccgcggaga tggtgccgcg gcttctgtct ggtcgcgagc agtggtgcca
     1981 gggcttctcc gagccgggat ccggcagcga cctggcgtcg ctgtccaccc gcgccactcc
     2041 acgcggcgat aactgggtcg ttaacgggca gaaggtctgg accagtttcg cgcagtattc
     2101 gcaacgatgc atcctgctca cccgaacagg gccgggccac aacgggatca cggcgttctt
     2161 cgtcgacatg gacacccccg gcatcaccgt tcggccgttg cgcacgatgc acggtgtcga
     2221 cgagttctgc gaggtttact tcgacgatgt ggtgattccc ggcgaccgga tgctcggcaa
     2281 gccgggtgac ggctggcagc tcgcgatgga cctgctgccc cacgagcgct ccacctgctt
     2341 ttggcagcga atcgcttatc tctacgcgcg attcgaccgg ctgattgccg aggcggacgc
     2401 gaggagcaag atagccgagg cggacgcgag gagcaagata gccgactcgg acgcaccggg
     2461 tgactccgag cttggctcgg cgtatctggc gctgcacact ttgcgctgcc gatcgcgtgc
     2521 cacgcagcac cggctcgccg acggggcgcg gctcggcccc gagacgtcga tcgacaaggt
     2581 gctgctggcc accgccgagc agaagctgta cgacaccgtg cgcgacctgc tgcccggcgc
     2641 ggtcgaactg gacgacacag aatggcgcac cgaatttctc tactcgcgtg cggcgacaat
     2701 ctacggcggc accgccgaga ttcaacgcaa catcatcgcg cgccggctac tcgacctcgg
     2761 gaaggagtga ccgtggatcc cgtctcgtcg gacgatatgg acctccttgc cgagacgctg
     2821 cgcaagacga tgaccgcgtc gtcgggccat gaactcgaca aggcactggc cgatctcggc
     2881 tggctcgaga tgctcgacga gatgccggag accgcaattc cgttggtgtt caagctgctt
     2941 ggggagaccg gagcacacgc ccctgtgttg aacgacgtgg tgctggccga ggcgggacgg
     3001 ccgtgcggcg aaacggtacc gctgccgttc gcgggcgggt cgtgggtggt gtggaatcgc
     3061 ggcgaccagc cgagttcagc gctggacccg gaaataccca tacgccgagt cacttcgaac
     3121 accccggtgc cgccggccga aaagcgctgg gctggtggct agttggcacc agtcgggcaa
     3181 tgctgtcgct ggtgcgccaa cacgcgctgg atcgcgtgca attcggccgt ccggtcgcgt
     3241 cgttccaagc gatccgtcac cggttggcgg agacgc
//
LOCUS       DASWBF010000123         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1198129,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000123 DASWBF010000000
VERSION     DASWBF010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198129"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1121)
                     /locus_tag="VF874_04230"
     CDS             complement(147..1121)
                     /locus_tag="VF874_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX9175324.1"
                     /translation="MTIAVTGSIATDHLMRFPGRFSEQLLADHLQKVSLSFLVDDLVV
                     HRGGVAGNMAYAIGALGGDAVLIGAAGMDFTDYREWLQAHGVNCDHVWISETAHTARF
                     VCTTDTEMAQIASFYPGAMSQARHISLKKIVSSTGTPELVIIGANDPDTMFLHTDECR
                     RLGLAFAADPSQQLARLSGEEIRKLIGGATYLFTNDYEWDLLLQKTGWTEDDVLRQVG
                     LRVTTLGPKGVDMVSPDGTSVHVDVVPENSKVDPTGVGDAFRAGFLTGRSAGLDLERS
                     AQLGSLVAVFVLETNGTQQWTWDCDVALTRLAGAYGQQAADEIGTALS"
     gene            1159..1755
                     /locus_tag="VF874_04235"
     CDS             1159..1755
                     /locus_tag="VF874_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175325.1"
                     /translation="MAGPYPQYSDPQLSQPPIAAASVYPGMLPPPVPYPSGRRWRLLA
                     VILLVVAIVGGATTAVVYALTADSHAPGGGQLTEAAAKAAIQDYLDAMEQGDTETVAR
                     NTQCGYYDEIKDKRSHQALARLTSETFRKQFSRAEVTSIDKMVFMSTNQAQVLFSMQV
                     APVSRGSKKHEEQAIAQVLSTDDSVLVCQYLLLSAGQY"
     gene            complement(1757..2119)
                     /locus_tag="VF874_04240"
     CDS             complement(1757..2119)
                     /locus_tag="VF874_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX9175326.1"
                     /translation="MTVQGESATGTATHGVILTDAAAAKAKALLDQEGRDDLVLRIQV
                     EPGGCAGLRYSLFFDDRSLDGDLTAEFGGVTLTVDRMSAPYMQGATIDFVDTIEKQGF
                     TIDNPNATGSCACGDSFN"
     gene            complement(2166..3257)
                     /locus_tag="VF874_04245"
     CDS             complement(2166..3257)
                     /locus_tag="VF874_04245"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX9175327.1"
                     /translation="MRVLIAPDCFGDSLSAVQAADAIAAGWRRARPGDELIPAPQSDG
                     GPGFVDVLAGRLGERRTARVSGPLDADVDAAWVFDTHPPPTAYVESAQACGLALLDGP
                     PTPQSALAAHTRGVGQLIAAALDAGAERIVVGLGGSATTDGGRGMVEALGGLAAAREL
                     LAGVELIAATDVEHPLLGPMGAARVFGPQKGADDATVTLLEHQLTDWAAELDAAAGRP
                     IRDLPGAGAAGGLGAALLALGGRRESGAAVIAAHTRLADDLAAADLVITGEGRLDDQS
                     LHGKVISALAAGAAGTPVLVLAGQVTLDESALRAAGVTGAFSLADYAGSVRRAIDDAA
                     NQLTGLAESAASKVSAASKVKEWGRTRYR"
     gene            3344..3946
                     /locus_tag="VF874_04250"
     CDS             3344..3946
                     /locus_tag="VF874_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HEX9175328.1"
                     /translation="MDVEPTESRKTTAPKGRPTPKRDAARRRGPVAPAPMTAAQARRR
                     RKEVGRKLSREERREFRAQQRQRTTERRDKMMAGEEGYLLPRDKGPVRRYVRDIVDSR
                     RNVLGLFMPIAFGLVFLMMAVPLPRVQLLISPAMMALVVVMIGSAFILGRKVNRLVDQ
                     KFPKNTETGWRLGFYAASRATQLRRMRAPRPQVNRGDTLT"
     gene            3952..>4019
                     /locus_tag="VF874_04255"
     CDS             3952..>4019
                     /locus_tag="VF874_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide kinase/adenosylcobinamide
                     phosphate guanyltransferase"
                     /protein_id="HEX9175329.1"
                     /translation="MRTLVLGGIRSGKSRWAEAAIA"
BASE COUNT          647 a         1429 c         1326 g          617 t
ORIGIN      
        1 tcaactcgcg catccaggcg atcaaggtct ctgcccgcgg ctaccgcaac cgtgagcact
       61 tcaagaccgc catctacttc cacctcggcg gattacagct ctacccggag ccatgaccca
      121 cggacttccc ggaagaaccg aaaaatctag gacagcgcgg tgccgatctc gtcggccgcc
      181 tgctggccgt acgcgccggc cagccgcgtc agcgcgacgt cgcagtccca cgtccattgc
      241 tgagtaccgt ttgtttccag cacgaacacc gcgaccagcg agccgagttg cgccgaccgc
      301 tccagatcga gccctgcgct gcggccagtg agaaaaccgg cgcggaacgc gtcgccgaca
      361 ccggtcgggt cgaccttgct gttctcgggc accacgtcga cgtggacact ggtgccgtcg
      421 ggactgacca tgtcaacacc cttgggcccc aacgttgtaa ctcgcagacc gacctgcctc
      481 agtacgtcgt cctcggtcca cccggtcttc tgcagcagca gatcccattc gtagtcgttg
      541 gtgaacaggt aggtggcgcc gccgatcagc ttgcgaatct cctcgccgga gagccgggct
      601 agctgctgcg atggatccgc cgcaaacgct agcccgagcc tgcggcactc gtcggtgtgc
      661 aggaacatcg tgtcaggatc gttggctccg atgatcacca attctggtgt gcccgttgac
      721 gatacgatct tcttcaacga gatgtggcga gcctgcgaca tcgcgcccgg gtagaacgac
      781 gcgatctgcg ccatctcggt gtcggtggtg cagacgaacc tcgcggtgtg cgcggtctcg
      841 gagatccaca cgtggtcgca gttcacgccg tgcgcctgca gccactcgcg atagtcggtg
      901 aaatccatgc ccgcggcccc gatcagcacc gcgtcgccgc ccagcgcccc gatcgcgtag
      961 gccatgttgc cggccacgcc gccgcgatgc accaccagat cgtcgacgag gaagctcagc
     1021 gacaccttct gcaggtggtc ggcgaggagc tgctcggaga accggcccgg aaatcgcatc
     1081 aaatggtcgg tggcaatgga gccggtcacg gcgatcgtca cgaggtctac ccctttggct
     1141 tgcgctagcc tgcgtaccat ggccggcccg tacccgcagt actccgaccc tcagcttagt
     1201 cagcctccca tagccgcggc gtcggtgtat ccgggcatgc tgccgccgcc ggtgccctat
     1261 cccagtggcc ggcgctggcg gctgctcgcg gtgatcctgc tggtggtcgc gatagtcggc
     1321 ggcgcgacca ccgcggtggt gtacgcgctg accgccgact cgcacgcccc gggcggcggc
     1381 cagctcaccg aggccgccgc gaaggcggcg atccaggact acctggacgc gatggaacag
     1441 ggcgacaccg agaccgtcgc ccgcaacacg cagtgcggct actacgacga gatcaaggac
     1501 aagcgatcac atcaggcgct ggcgcgcctg accagcgaaa ccttccgcaa gcagttctcc
     1561 cgggccgaag tcacgtcgat cgacaagatg gtgttcatgt cgacgaacca ggcgcaggtg
     1621 ctgttctcca tgcaggtggc gcctgtgtcg agagggtcga agaagcacga agagcaagcg
     1681 atcgcccagg tgttgtccac cgacgacagc gtgctcgtgt gccagtacct gctgctgtcg
     1741 gccggtcagt actgagctag ttgaacgagt cgccgcaggc gcacgagccg gtggcgttcg
     1801 ggttgtcgat cgtgaagccc tgcttttcga tcgtgtcgac gaagtcgatg gtcgccccct
     1861 gcatgtaggg cgcgctcatc cggtcgaccg tcagcgtgac gccgccgaat tcggcggtga
     1921 gatcgccgtc cagcgaccgg tcgtcgaaga agaggctgta ccgcaggccc gcgcagcccc
     1981 ccggctcgac ctgaatcctc aagaccaggt cgtcacggcc ttcctggtcg agcaacgcct
     2041 tggctttcgc cgccgccgcg tcggtcagga tcacgccatg ggtggcggtg ccggtggccg
     2101 actccccctg aacagtcatt gcgtctcctc aagattttct cgcccgaacc ctcgagctcg
     2161 cttgttcaac ggtaccttgt tcggccccat tccttcactt tcgaggccgc cgacactttc
     2221 gaggccgccg actccgcaag tccggtcagc tgattcgccg cgtcgtcgat ggcccggcgc
     2281 accgagccgg cgtaatccgc gagcgagaag gcgccggtca cgccagctgc acgcagcgcc
     2341 gactcgtcga gggtgacctg acctgccagc accagcactg gtgtcccggc cgcccctgcc
     2401 gcgagcgcgc tgatcacctt gccgtgcagc gactggtcgt cgagccggcc ttcgccggtg
     2461 atcacgaggt cggcggcggc caggtcgtcg gccagccggg tgtgcgcggc gatcaccgcg
     2521 gcaccggact cccgccgccc gcccagcgcc agcagtgctg cgcccagccc tcccgcggcg
     2581 cctgcgccgg gcaggtcgcg gatcggacgg cccgcggccg cgtccagctc ggcggcccag
     2641 tcggtgagct ggtgctccag cagcgtgacc gtggcgtcgt cggcgccttt ctgtggcccg
     2701 aacacccgtg ccgcgcccat cggcccgagc agcgggtgct cgacgtcggt ggcggcgatc
     2761 agctcgacgc ccgccagcag ctcgcgcgcg gcagccagtc ccccgagcgc ctcgaccatg
     2821 ccacggccgc cgtcggtagt ggcgctgccg cccagcccga ccacgatccg ctcggcgccg
     2881 gcgtccagcg cggcggcgat cagctggccg acccctctgg tgtgcgccgc cagcgcactc
     2941 tgcggcgtcg gcggcccgtc gagcagcgcc agcccgcacg cctgcgccga ctcgacgtac
     3001 gcggtcggag gggggtgggt gtcgaacacc caggcggcgt cgacgtcggc gtccagcggc
     3061 ccggacaccc gcgccgtcct gcgctccccc aaccggccgg cgaggacatc gacgaatccg
     3121 gggccgccgt cggactgcgg cgcggggatc agctcgtcgc cgggacgcgc ccggcgccag
     3181 ccggccgcga tcgcgtcggc ggcctgcacc gcggacagcg agtcgccgaa gcagtcggga
     3241 gcgatcaaca cgcgcatgat caccgcacct taaggcgaag taccctggcg acgtgagtct
     3301 gctgggccga aagaagcacg aaaccgacga ggacgccgtc gacctggatg tcgagcccac
     3361 cgaatcccgc aagaccaccg ctccaaaggg ccggccgacg cccaaacgcg acgccgccag
     3421 acgccgcgga ccggtcgcgc ccgcgccgat gaccgccgcg caggccaggc gccggcgcaa
     3481 ggaggtgggc cgcaagctca gccgcgagga acgccgcgaa ttccgggctc agcagcgtca
     3541 gcgtacgacg gagcgtcgcg acaagatgat ggccggcgag gagggctact tgctgccccg
     3601 cgacaagggg ccggtgcgcc gctatgtgcg cgacatcgtg gactcgcggc gcaacgtcct
     3661 cggcttgttc atgcctatcg cattcggctt ggtgtttctg atgatggcgg tcccgctgcc
     3721 cagagtgcag ctgctgatct cccccgcgat gatggcgctg gtggtggtca tgatcggcag
     3781 tgcgttcatt ctcggccgca aggtgaacag gctggtcgac cagaagttcc cgaagaacac
     3841 cgaaaccggt tggcggctag gcttttacgc tgcgagccgg gccacgcagc tacgccgcat
     3901 gcgggcgccg cgaccgcagg tcaaccgcgg cgacaccctc acctgaccgc cgtgcggacg
     3961 ctggtgctcg gcggcatccg gtcgggcaag tcgcgatggg cggaagcggc gatcgccga
//
LOCUS       DASWBF010000124         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_341345,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000124 DASWBF010000000
VERSION     DASWBF010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341345"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..372
                     /locus_tag="VF874_04260"
                     /pseudo
     CDS             223..372
                     /locus_tag="VF874_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(439..1176)
                     /locus_tag="VF874_04265"
     CDS             complement(439..1176)
                     /locus_tag="VF874_04265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9175330.1"
                     /translation="MANIDRRTMLGAIALGVAAALNRTAAAGASPVRWTVPLGRLPAP
                     TGVLTRLPGDGNQLALTVDDGASTSVVAAFAQLCRDTGTRLTFFVNGANNSWSANAPA
                     LRPMVDSGQIQMANHTWSHPYLNRLAPTAVAEQIRRNADFLRNTYGNDGAPFFRPPYG
                     VHTPDSDRIAADQGYSTITLWSGTVGDSTPENEAALIANAARSFQPQQIVLTHANLPT
                     ITHCYDQLLDLISKRNLQTVTLNDVFG"
     gene            complement(1341..>1599)
                     /locus_tag="VF874_04270"
     CDS             complement(1341..>1599)
                     /locus_tag="VF874_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020106485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175331.1"
                     /translation="AGLGGFYLHTVALQLGSVNGATAALVVGETVVPGITGVVLLGDT
                     AERGLGWLVAVGFVGAVAGAVTVAIFGAADQAPAKSPTRDQ"
BASE COUNT          285 a          535 c          522 g          257 t
ORIGIN      
        1 tccagcgcat gctcgtcagc atcccgctca ccgacgacga acaaaccgcc gtcgaagacg
       61 gtcaagccgc cctcgaccac ctactgcaac gcctcgccga catcccgact ccggccggga
      121 ccacaccacg acaactgacc ggcgccacct cgctgccgat cgtcgccgtc aacccacggc
      181 cgatcagcac gaacttgaca atcctgattc cacagaacga ccgagcaggc ggcgctcaag
      241 tgcgtctacc tggcgatcat gagcctcgat ccgaccggca agggccgaaa gcgctgggtc
      301 agccgatgga aaactccgct caacgccttc gaactcgctt tccccggacg actcaccacg
      361 agccgaaact aacagtacga caacgagtta caccgttagc ttgacagtcc catctgctcg
      421 tgcaggtggc gggtgggctc agccgaacac gtcgttgagc gtgacggtct gaagattgcg
      481 cttagagatc agatcgagca attggtcgta gcagtgggtg atggtcggca ggttggcgtg
      541 tgtcagcacg atctgctggg gctgaaacga cctggcggcg ttggcgatca acgctgcctc
      601 gttttccggg gtcgagtccc cgacggtgcc gctccacaag gtgatcgtgg agtagccctg
      661 gtcggcggcg atccggtcgc tgtcgggggt gtgcacgccg tagggcggcc gaaagaatgg
      721 ggctccgtcg tttccgtagg tattgcgcaa gaagtcggcg ttgcgcctga tctgctcggc
      781 caccgcggta ggtgccaacc ggttgaggta gggatgtgac caggtgtggt tggccatctg
      841 aatctggccg gaatccacca tcggcctcag cgccggcgcg ttggccgacc atgagttgtt
      901 ggcgccgttg acaaagaagg tcagccgtgt cccggtgtct cggcagagtt gggcgaaggc
      961 cgccaccacc gaagtgctcg cgccgtcgtc gacggtcagc gccagctgat taccgtcgcc
     1021 gggcagccgg gtgagcaccc cggtcggcgc gggcagtcgg ccgagcggga ccgtccagcg
     1081 aacgggcgaa gcaccagcgg cggctgtccg gttcagggcc gccgccaccc cgagggcgat
     1141 cgcgcccagc atggtccgcc gatcaatgtt ggccatgccg ctagtgtgcc gcggcgcggc
     1201 cccgggcgtg agcggttttg tattccgccg cttgatgttt ggctcaggga gaccatcgcc
     1261 acaccgccgt aggtggtagg aatcccttgt ctggccgtgg gcgctgctcc cgacagcagc
     1321 cacgcgccag aggcgcagcc tcactggtcc cttgtgggtg atttcgccgg cgcctggtcg
     1381 gcggctccga aaatggcgac ggtcaccgca cccgcgaccg cgccgacgaa ccccacggcc
     1441 accagccagc cgagcccgcg ctcagcggtg tcgccaagca gcaccacgcc ggtgataccg
     1501 ggcaccaccg tctcaccgac gacgagggca gcggtagcac cgtttaccga accgagctgc
     1561 agcgccacag tgtgcaggta gaagccgccc agcccggcg
//
LOCUS       DASWBF010000125         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_668617,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000125 DASWBF010000000
VERSION     DASWBF010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668617"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..211)
                     /locus_tag="VF874_04275"
                     /pseudo
     CDS             complement(1..211)
                     /locus_tag="VF874_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611776.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            335..1420
                     /locus_tag="VF874_04280"
     CDS             335..1420
                     /locus_tag="VF874_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9175332.1"
                     /translation="MPFGDPESWTLVDRDSAVVEPVEGFLSHLHAVERSPNTVRAYAH
                     DLRDWFEFLDQRGVVWSRVRLEDVGRFVAWLRLPAAGRVGNVSALPTAENVRSEVTVN
                     RKLSAISAFYEFHQRHGVDLGDLLTTWQRRKTHGGSWQPLLAHLGSRPERTRRIRLRA
                     QRRIPDTLDGEQVAAIVAACDRLRDRFLFTLLGASGLRVGEALGLRHSDIDAAARLVT
                     VVSRVNSNRARAKGTGGRRVPVPAQVIRLYADYLHNEYGDLDSDYVFVTLWSGPIGHP
                     LTYPSVYDLVCRIRERTGITFGPHTFRHTYATELLRRGVAVEVVQHLLGHTSIATTSD
                     AYAHLKVEDARRALVAAGWLTDQDRSR"
     gene            1417..3663
                     /locus_tag="VF874_04285"
     CDS             1417..3663
                     /locus_tag="VF874_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9175333.1"
                     /translation="MSVEALAAPSAPTLAQRLDATVRAEFRVEVLVPAVGDPILGSPE
                     CTVPGCIHSSRYAGLCPAHLGRWRKAGRPDRRAWVATADPAVAGHRPLRSCLVPECGF
                     GQHRYRLCYKHSHTWDKAGRPPMQRWQSPITSPAKVCAIPGCSLWAELDAGWCRSHHA
                     RWRQRGRPPADEFIAYCAGYGEDRFDFGLLPPQMRLEIQYVLQCRVDANRTRTTPRSI
                     KPLLDHLAASGAGSLLDRPLAEWVAGLPAAAALHTPRAFLGYAIECLLDLRDGTGWDS
                     EYERDVWLLRRLGVVGHDGARLDFTAVQPLWLRELAKRWCRWRMSCGVGLGQVRTDRM
                     ALVRLSQLTPGLAGSSGPRALDRAALEAYLARLAVALPHPKTRSTDIGAVTGFLHAVR
                     QHRWASLPAEAQLYPSDHPRRDETPTPRAIPEFVLHQLEDPANLDRITDPRIRLLVEI
                     LIRTGLRIGDATRLALDCLVRDPQGAVYLRYRNHKMRRDAVVPIDDELTTMIEAQQAR
                     TRRRFPTTGVLLPRGSANPDGRLSIPTATFHRQLGIWLETCGVTDEFGRPVHVTAHQF
                     RHTAATRWINHDVPQEVVRRLLDHTSHTMTAVYARLADTTIREQWERAQKINIRGEPV
                     DTSPDGPLADAEWMKQNLARAKMALPNGYCGLPLQKSCPHANACLTCPLFVTTAEFLP
                     QHRKQLVDTRALIARAQTDGHTRLAEMNRTVETNLLTIITTLERRHRCSCGNGETCCG
                     EGGSDGTS"
     gene            3650..4111
                     /locus_tag="VF874_04290"
     CDS             3650..4111
                     /locus_tag="VF874_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HEX9175334.1"
                     /translation="MAPADNTRHLVAAAQRRRTDTLDRARKTLQELSETGQRHTVTEI
                     AARAGVSRAWLYAQAELRDDIRRLTTAATAPAPAADRIERGTDASLRQRLTLAHERIR
                     ELDTENRQLRDQVAQLHSQLRAARIGNIHVADTVNDANPQLTPANARTGQR"
BASE COUNT          669 a         1405 c         1394 g          650 t
ORIGIN      
        1 ccacagcgcc cgtttcgacg gtgctcgacg agcagtgcgc ccagtagctg cgcggggcga
       61 tctgcacgcc gcgcacggcc agtgcggcag atcggtacga ccccgtacgt gcccttgtgc
      121 tcgtcgatga actgacagat cagcggtgtc gcgggtcgca ctcccgcgcg aagaaacttg
      181 ttgcggcctt gaggatttcg atagttatct gaggccctaa ggagaccgtc cacgatgtag
      241 ccgacacgcc gcctggggtg ccgggaaatg cgctgtggac tggttatctt cggtgtctgt
      301 gacgaagcct ggtctgcggg tgcagccggt ggtgatgccg ttcggcgacc ctgaatcgtg
      361 gacgctggtg gatcgggatt cggcggtggt ggagccggtc gaggggttct tgtcgcatct
      421 gcacgcggtg gagcggtcgc cgaacacggt gagggcgtat gcgcacgatc tgcgcgactg
      481 gttcgagttc ctcgatcagc gtggcgtggt gtggtcgcgg gtgcgactgg aggacgtggg
      541 ccggttcgtg gcgtggctgc ggctgcccgc ggcggggcgg gtgggcaatg tgtcggctct
      601 gccgaccgcg gagaacgtgc gctcggaagt gacggtgaac agaaagctgt cggcgatctc
      661 ggcgttttac gagtttcatc agcgtcacgg cgtggatctg ggggatctgc tgacgacttg
      721 gcagcggcgc aagacgcatg gtggatcgtg gcagccgctg ctggcgcact tggggtcgcg
      781 accggaacgg accaggcgga tccggctacg ggcgcagcgg cgcatcccgg acacgctcga
      841 cggggagcag gtcgcggcga tcgtggcggc ctgtgatcgg ctgcgggacc ggttcctgtt
      901 tacccttttg ggtgcgtccg ggctgcgggt cggcgaggca ttggggttgc ggcacagcga
      961 catcgacgcc gcggcgcgac tggtgacggt ggtgtcgcgg gtgaactcga accgggcgcg
     1021 ggccaagggc acgggaggcc ggcgagtgcc ggtgccggcg caggtgatcc ggctctacgc
     1081 cgattaccta cataacgagt atggcgacct ggacagtgac tatgtgttcg tcaccctgtg
     1141 gtcgggcccg atcgggcacc cgttgaccta cccgagtgtc tacgatctcg tctgccggat
     1201 ccgggagcgg accggaatca cgttcgggcc gcacaccttt cgccacacgt acgcgactga
     1261 gctgctgcgt cgcggcgtgg cggtggaggt ggtgcagcac ctgttggggc atacgtcgat
     1321 cgccaccacc agcgacgcgt acgcgcacct gaaggtcgag gacgcaaggc gcgctctcgt
     1381 cgccgcgggg tggctgaccg accaggatcg gtcgcggtga gcgtcgaggc gctcgccgca
     1441 ccatccgcgc caaccctggc gcagcgtttg gatgcgacgg tgcgtgcgga gttccgggtc
     1501 gaggtgctgg tgcctgcggt gggtgatccg attctcggtt cccccgagtg cacggttccc
     1561 ggctgcatac actcaagccg ctacgccggg ctgtgtccgg cgcatctggg ccgctggcga
     1621 aaggctggtc gcccggaccg acgggcgtgg gtagcgaccg ccgatccggc ggtggccggt
     1681 caccggccgc tgcggtcgtg tctggtgccc gagtgcgggt tcggtcagca tcgatatcgg
     1741 ttgtgctaca agcattccca tacctgggac aaggccgggc gcccaccgat gcaacggtgg
     1801 cagtcgccaa tcacgagtcc ggcgaaggtg tgcgcgatcc cgggctgctc attgtgggcg
     1861 gaactggacg ccggctggtg ccgatctcac cacgcccgct ggcggcagcg cggtcgccca
     1921 ccggctgacg agttcatcgc ctactgcgcc ggctacgggg aggaccgctt cgacttcggg
     1981 ctgttgccgc cgcagatgcg cctcgagatc cagtacgtgc tgcaatgccg ggtggacgcg
     2041 aaccgcaccc gcaccacacc ccgatcgatc aaaccgctgt tggatcacct cgctgccagc
     2101 ggcgctgggt cgctgctgga ccggccgctc gccgaatggg tggccgggtt gccggccgcg
     2161 gccgcactcc acacgccgcg cgcgttcctc ggctacgcca tcgagtgcct gctcgacctg
     2221 cgcgacggga ccgggtggga cagcgagtac gagcgggacg tgtggctgct gcggcggttg
     2281 ggtgtcgtcg gccacgacgg agctcggttg gatttcactg cggtgcaacc actgtggttg
     2341 cgcgagctgg ccaaacgctg gtgccgctgg cgcatgtcgt gtggtgtcgg gctgggccag
     2401 gtgcgcacgg accggatggc gctcgtccgc ttgtcgcagc tgacaccggg attggcaggc
     2461 tcatcgggcc cgcgtgcgct cgatcgggca gcgctggagg cctacctggc gcggctcgct
     2521 gtcgcactcc cacacccaaa gacccgcagc accgatatcg gcgccgtgac cgggtttctg
     2581 cacgcggtgc gccagcaccg ctgggcgtcg ctgcccgcgg aggcgcagct gtatcccagc
     2641 gaccatcctc gccgcgacga gacaccgacc ccgcgggcga ttcccgagtt cgtcctgcac
     2701 caactcgaag accccgccaa tctcgaccgg atcaccgacc cgcgaatccg gctgctggtg
     2761 gagattctca tccgcaccgg cctgcgcatc ggcgacgcca cccggctggc gctggattgc
     2821 ctggtccgtg acccgcaggg cgcggtctat ctgcgttacc gcaaccacaa gatgcgtcgc
     2881 gatgcggtgg tccccatcga cgacgaactc accaccatga tcgaggcgca acaggcccgc
     2941 acccggcggc ggttcccgac caccggtgtg ctgctgccgc gcggcagcgc caaccccgac
     3001 gggcggctgt ccatccccac tgcgaccttc caccgccagc tcggaatttg gcttgaaact
     3061 tgcggtgtca cagatgaatt cgggcgaccc gtgcatgtca ccgcccatca gttccggcac
     3121 acggcagcca ctcggtggat caaccatgac gtgccgcagg aggtggttcg acggctgctc
     3181 gaccacacca gccacaccat gacggccgtg tatgcccggc tggccgacac cacgatccgg
     3241 gagcagtggg aacgtgccca gaagatcaac atccgcggtg agccggtcga caccagcccc
     3301 gacgggccgc tcgccgatgc cgaatggatg aaacagaacc tcgcccgcgc gaaaatggcg
     3361 ctgccgaacg gctattgcgg tctgccgcta cagaaatcct gtccgcacgc caacgcgtgt
     3421 ttgacctgcc cgctgttcgt caccacagcc gaattcctgc ctcagcaccg caagcaactc
     3481 gtcgacaccc gcgcgttgat cgcccgcgcc cagaccgacg gccacacccg cctcgccgag
     3541 atgaaccgca ccgtcgaaac caacctgctc accatcatca ccaccctcga acgccgacac
     3601 cgctgcagct gcggcaacgg cgagacatgc tgcggcgaag gaggatccga tggcaccagc
     3661 tgacaacacc cgccacctcg tcgccgccgc ccagcgccgc cgaaccgaca ccctcgaccg
     3721 cgcccgtaag accctgcagg aactcagcga gaccgggcag cgacacaccg tcaccgagat
     3781 cgccgcccgc gccggcgtct cccgcgcctg gctctacgcc caggccgagc tccgcgacga
     3841 catccgacgg ctcaccaccg cagcgaccgc gccggcacca gccgccgacc ggatcgaacg
     3901 cggaaccgac gcttcgctgc ggcagcgact caccctcgcc cacgagcgca tccgcgaact
     3961 cgacaccgag aaccgccagt tgcgcgacca ggtcgcgcaa ctgcacagcc aattacgagc
     4021 cgcccgcatc ggcaacatcc acgtcgcaga cactgtcaac gacgcaaacc ctcagctcac
     4081 gccggcaaac gcccgaaccg gccaaagata acgatagt
//
LOCUS       DASWBF010000126        11287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_273125,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000126 DASWBF010000000
VERSION     DASWBF010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273125"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VF874_04295"
     CDS             complement(<1..797)
                     /locus_tag="VF874_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9175335.1"
                     /translation="MTSIQQRDAQSVLTAVDDLLPKLRQRAQETEDLRRLPDATVSEL
                     QEIGFFRLLQPEQWGGLQCNPALFYEAARRLASACGSTGWVSSIVGVHNWHLALFDQQ
                     AQEEVWGEDTNVRISSSYAPMGAGEVVDGGYLVNGSWNWSSGCDHATWTFVGGPVIKD
                     GRPVDFGSFLIPRTEYQIDDVWHVVGLRGTGSNTLVVKDVFVPRHRFLSYKSMNDHTA
                     GGLQTNTAPVYKMPWGTVHPTTISAPIVGMAYGAYDAHVEHQGKRIRA"
     gene            938..1993
                     /locus_tag="VF874_04300"
     CDS             938..1993
                     /locus_tag="VF874_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX9175336.1"
                     /translation="MTDEPLGSHVLELQVAEVVAETDDARSLVFKVPDGAEIAPQKLV
                     YAPGQFLTLRIPSDRTGSVARCYSLCTSPFTDDAMAVTVKRTDGGYASNWLCDHAHPG
                     MKLHVLAPSGTFVPPTLDADFLLIAGGSGITPMMAICKSALAEGGGKIVLVYANRDEN
                     SVIFGGALRELAAKYPDRLTVVYWLESVQGIPTETALASLTAPYASHDAYICGPGPFM
                     QAAQAALGTIGVPKERVHLEVFRSLDTDPFAAVVIEDDSDEPPATAIVHLDGQRHEIS
                     WPRNAKLLDVLLDKGLDAPFSCREGHCGACAVLKRSGEVEMEVNDVLEQSDLDEGLIL
                     GCQARPRTDSVEVTYDE"
     gene            2038..2571
                     /locus_tag="VF874_04305"
     CDS             2038..2571
                     /locus_tag="VF874_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175337.1"
                     /translation="MKRLSVLIAGGLLASGLGVPGVASAASDNGDTSFPLPDGTALEV
                     HVTANCVSAEKQCYFTVAANRLSGDGVDGFPGDLWARQTTTLRSSNRLNNLETQVVAA
                     NTRQYKQIGRREITTIYFGGGPAEKYQISGQTQPTNWQDGQPMLNADYIVCAEIQVVY
                     SGVNVTSPTTCAQTTFS"
     gene            complement(2654..4786)
                     /locus_tag="VF874_04310"
     CDS             complement(2654..4786)
                     /locus_tag="VF874_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9175338.1"
                     /translation="MSASISDEQTAARELVRSWAPASGAIAAARAVEQGDTDAWLKPF
                     SGLAELGFFGVAVPESLSGAGGGVADLCAMLEEAAYALVPGPVTTTALATLLLDDEQV
                     LGELMSGQRSAGVALDGDLRYESGQVSGTLPVVLGARPGEYVIAPAGSQWLLIDTGSA
                     GATVEPLTPTDFSRPLARVVLDSVPAAAPDVATQWVEDLVATVLAAEATGLARWMLDT
                     ATEYAKVREQFGKPIGSFQAIKHLCAEMLLRSEQASVAAADAARAASEGFDSQLSIAA
                     AVAAAVGIEAAKANAKDCIQVLGGIGITWEHDAHLYLRRAHAISQFLGGKSRWLRRVA
                     ALTRDGVRRELHVDLESVDHLRPEIAAALDDVAALPSDKRQVALAEAGLLAPHWPKPY
                     GREASPAEQLLIDQELAAAGIERPDLVIGWWAAPTILEHGSPKQIECFVPATLRGELI
                     WCQLFSEPGAGSDLASLRTKAVRAQGADARSKNGTGWRLTGQKVWTSAAHKAQWGVCL
                     ARTDPDAPKHKGITYFLVDMKSEGITIRPLREITGDELFNEVFFDDVFVPDEMVVGAV
                     NDGWRLARTTLANERVAMAQGTALGNPMEELLATVANVDLDAADEDRLGELIVTAQAG
                     SLLDQRIAQLAVGGQDPGPQASARKLIGVRYRQGLAEFRMDLSEGAGIVENRQVHDFL
                     NTRCLTIAGGTEQILLTMAAERLLGLPR"
     gene            5050..5655
                     /gene="kstR"
                     /locus_tag="VF874_04315"
     CDS             5050..5655
                     /gene="kstR"
                     /locus_tag="VF874_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol catabolism transcriptional regulator
                     KstR"
                     /protein_id="HEX9175339.1"
                     /translation="MAALAESELGSEAQRERRKRILDATLAIASKGGYEAVQMRAVAE
                     RADVAVGTLYRYFPSKVHLLVSALGREFERIDAKTDRMTLGGGTPYQRLHQMISRLNR
                     AMQRNPLLTEAMTRAFVFADASAAGEVDHVGKLMDSMFARAMSDGEPTEDQYHIARVI
                     SDVWLSNLLAWLTRRASATDVSKRLDLAVRLLIGDEEHPKI"
     gene            complement(5657..6751)
                     /locus_tag="VF874_04320"
     CDS             complement(5657..6751)
                     /locus_tag="VF874_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9175340.1"
                     /translation="MSRPSVHRRRATLASLAAELKVSRTTISNAYNRPDQLSADLRER
                     VLETAKRLGYPGPDPVARSLRTRKAGAVGLVLTEPLNYAFSDPAAVSFVAGVAESCEE
                     VGQGLLLLAVGPGRSIEEGSHAVLNAGVDGFVVYSVADDDPYLEVALKRNLPVVVVDQ
                     PKNVPGTSRVCIDDRAAMRELADYVVGLGHREIGLLTMRLGRERTPGAAAVASPERLR
                     FPHFHVQRERISGVREAMEAAGVDPDSLTVVECYEHLPTSGGAAAEVALEANPGITAL
                     MCTADVLALSAMDYLRARGIYVPGQMTVTGFDGVPAALGRGLSTVTQPNLEKGRLAGE
                     LLHTPPRSGLPVDEVLPTELVRGRTAGAPG"
     gene            6845..7732
                     /locus_tag="VF874_04325"
     CDS             6845..7732
                     /locus_tag="VF874_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX9175341.1"
                     /translation="MGRAMIWLVAALVAVSACGSPPDQADKVSVVASTSVWGAIAEVV
                     GGDYVQVRSIIDSPAEDPHSYDVSPADAAAIADAQLVVYNGGGYDNFVDAVLEQHDAV
                     KRVDAFTVGGHQQGSNPHVFYDLGTVSLLAGAVADQLAAVDPTHATDYRSNAEKFTDQ
                     VHGIEATQRGIAAEHVGSAAIATEDIAQYLEAAVGLTDRTPEGFFKAVAADFDPGPAD
                     IAAVLDTVNSHGVQVVLFNPQTDTAVTRRIVDAASTAGVPVVEVRETLPAATDFLTWQ
                     RHTVEQLSAALQRADPHTP"
     gene            7729..8517
                     /locus_tag="VF874_04330"
     CDS             7729..8517
                     /locus_tag="VF874_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX9175342.1"
                     /translation="MNAPLVELRAARLSFGDRALWDGLDLTVGAGEFVAILGPNGCGK
                     TSLLRVLTGQLPLTEGSAYIAGRPVSSGRSTVGYIPQHGASERGLTMRGGDLVRLGVD
                     GHRWGLTGRRAARLQRDLAVEAALSQVAATHLARTSLSVMSGGELQRLRIAQALAGDP
                     VLLLCDEPLLNLDPASAEQVSELIDKRRRDAGTAVLFVTHEINPVLPYVDRVLYLVDG
                     RFRIGTVDEVMTSENLSALYRADVDVVHVHGQYVVVGNRGFCHE"
     gene            8510..9376
                     /locus_tag="VF874_04335"
     CDS             8510..9376
                     /locus_tag="VF874_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX9175343.1"
                     /translation="MSDSLNSLLHNMFAFHVTADLLGRGFVQQAIAAGALLALVSGLI
                     GPFIVMRQMSFAVHGSSELSLTGAAFALLTGLNVGAGALVGSLIAAVLFGILGQRARE
                     RDSAIGVVLAFGLGLAVLFIHLYPGRAGTSFALLTGQIVGVGYSGLAQLVGVAVIVAI
                     VLTVTGRPLLFATVDPEVASARGVPVRGLGIVFAALVGVVAAQGVQIVGALLVMSLLI
                     TPAAAAARLFSSPSATIAASVIFAEFAAVGGIVLSLAPGVPISVFVTIISFAIFVVCW
                     LVERRRRPVLHA"
     gene            9408..10155
                     /locus_tag="VF874_04340"
                     /pseudo
     CDS             9408..10155
                     /locus_tag="VF874_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
     gene            10293..>11287
                     /locus_tag="VF874_04345"
     CDS             10293..>11287
                     /locus_tag="VF874_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9175344.1"
                     /translation="MRWTGENDPVVTPFIRSQLAVFLALTVVALGALAFHYVRLPQKV
                     GIGQYTLYVDLPRAGGLYATGAVTYRGATIGQVTKVEPTERGARATLSINNRYKIPLD
                     TSANVHSVSAVGEQYLDLVSPTGDASQHFRDGQTITNSTVPSEVGPALDAANQALAAL
                     PTDKIDNLLTEASQAVGGLGPALQRLVDSTTAIASDFRDNLAPVNDIIAHSAPILDSQ
                     VNSGDAIGAWSANLNVIVAQAAEQDAALRSGLQQAAPTADQLNAVFGDVREALPQTLA
                     NLAIVTDMLKRYHKGLEQALVILPQGATVAQAGTIFPGEGLLHFGLSINQPPPCLT"
BASE COUNT         1841 a         3759 c         3778 g         1909 t
ORIGIN      
        1 gctcggatcc tcttgccctg gtgctccacg tgtgcgtcgt aagcgccgta agccatgccg
       61 acgatgggcg cagaaatcgt ggtcggatgc acggtgcccc aaggcatctt gtacaccggc
      121 gcggtgttgg tctgcagccc gcccgcggtg tggtcgttca tcgacttgta ggacaggaag
      181 cggtgccgcg gcacgaacac gtccttgacc acgagcgtgt tgctgccggt gccacgcagc
      241 ccgaccacat gccacacgtc gtcgatctgg tactcggtgc gcggaatcaa gaagctgccg
      301 aagtccaccg gcctgccgtc cttgatcacc gggccgccga cgaacgtcca cgtggcgtgg
      361 tcgcagcccg acgaccagtt ccacgagccg ttgaccagat agccgccgtc aacaacctca
      421 ccggcgccca tcggggcgta cgacgacgag atccgcacgt tggtgtcctc gccccacacc
      481 tcttcctggg cctgctggtc gaacagcgcg aggtgccagt tgtgcactcc gacgatcgag
      541 ctgacccacc ccgtcgaccc gcacgcactg gcaaggcgac gggcggcctc gtagaacagc
      601 gccggattgc actgcaggcc gccccactgc tcgggctgca gcaaccggaa gaagccgatc
      661 tcctgaagtt cgctgactgt ggcgtccggc aggcggcgca ggtcctcggt ctcctgggcg
      721 cgctgccgca gcttgggcag caggtcgtcg acggcggtta ggaccgactg ggcgtcgcgc
      781 tgttgaatgg acgtcactgg ttccctccat tggtccgggg cttagtcgca agattagaac
      841 acgttacgat tttagtcgag tagggcatac ctgcaggggg tagacctgcg gaagtaggtt
      901 tctgtaacct gttctagtta tatgcggaag gagagtcgtg accgacgagc cgctcggcag
      961 ccacgtgttg gaactgcagg tggctgaagt cgtcgcagag accgacgacg cgcgctcgct
     1021 ggtgttcaag gtgcccgacg gcgccgagat cgccccgcag aaactggtgt acgcgcccgg
     1081 acagttcctg acgctgcgca ttcccagcga ccgcaccggc tcggtcgccc gctgctactc
     1141 gctgtgcacg tcgccgttca ccgacgacgc gatggccgtc acggtcaagc gcactgacgg
     1201 cggctacgcg tcaaactggc tgtgcgacca cgcacacccg ggcatgaagc tgcacgtgct
     1261 tgctccgtcg ggaacgttcg tgccgcccac gctggatgcc gacttcctgc tgatcgccgg
     1321 cggcagcggc atcacgccga tgatggcgat ctgcaagtcg gccctggccg agggcggcgg
     1381 caagatcgtg ctcgtctacg ccaaccgcga cgagaactcg gtgatcttcg gcggtgcgct
     1441 gcgcgaactg gcggccaaat accccgacag gctgaccgtc gtgtactggc tggaatcggt
     1501 gcagggcatc cccacagaga ccgcgctcgc cagcctgacg gcgccgtatg cgtcccacga
     1561 cgcctacatc tgcggacccg gaccgttcat gcaggccgcg caggccgcgc tgggcacgat
     1621 cggtgtgccc aaagagcgcg tgcaccttga ggtgttccgt tcgttggaca ccgacccgtt
     1681 cgcggcggtg gtgatcgagg acgacagcga cgagccgcca gcaaccgcga tcgtgcacct
     1741 cgacggccag agacacgaga tcagctggcc gcgcaacgcg aagcttctcg acgtgctgct
     1801 ggacaagggg ctcgacgcgc cgttctcgtg ccgcgagggt cactgcggcg cgtgcgcggt
     1861 actcaagcgc agcggcgagg tcgagatgga ggtcaacgac gtgctggagc agtcggacct
     1921 ggacgagggg ctgattctgg gctgccaggc ccggcctcgg acggattcgg tggaagtcac
     1981 ctacgacgaa tagcgcccgg ctaacatttc gcccaccgac gcttggggag gaacaccatg
     2041 aagcgactgt cggtactcat cgcggggggc ctgctcgctt cgggactcgg cgtcccgggc
     2101 gtcgcgtcgg cggcctccga caatggcgac acgtcgtttc cgcttcccga cggcactgcg
     2161 ttggaggtgc acgtcaccgc caattgcgtt tcggcggaaa aacagtgcta cttcacggtt
     2221 gcggccaacc gcctcagcgg tgacggcgtc gacggtttcc ccggcgatct gtgggcccgc
     2281 caaaccacca cgctgcggtc gtcgaaccgg ctcaacaatc tcgaaacaca agtcgtcgcc
     2341 gccaacaccc ggcagtacaa gcagataggc cgccgggaga tcaccacgat ttacttcggt
     2401 ggcggcccag ccgagaagta ccagatctcg gggcagacgc agccgaccaa ctggcaagac
     2461 ggccagccga tgcttaacgc cgactacatc gtctgcgcgg agatccaagt cgtctactcc
     2521 ggggtgaacg tcacgtcgcc gaccacctgc gcgcagacga cgttttccta agttctcctg
     2581 acgccgagcg tgcggccact gcgaaaatct gcgcccgatt attcgcactg ggcgcacgtt
     2641 cgcggcaaag gggtcagcgc ggtaggccga ggagtcgctc ggcggccatc gtgagcagga
     2701 tctgttcggt gccgccggcg atcgtcaggc agcgggtgtt gaggaagtcg tgcacctggc
     2761 ggttctcgac gatgccggcg ccctcggaca ggtccatccg gaactcggcc agcccctgcc
     2821 ggtatcgcac accgatgagc ttgcgggcac tggcctgcgg gcccggatcc tgaccaccca
     2881 ccgccagttg cgcgatccgc tggtccagca gtgagccggc ctgcgccgtg acgatcagct
     2941 cgccgagccg gtcctcgtcc gcggcatcga ggtcgacgtt cgccaccgtc gccagcagct
     3001 cctccatcgg attgccgagc gccgtgccct gggccatcgc gacgcgttcg ttggccagcg
     3061 tcgtgcgggc caaccgccag ccgtcgttga ctgcgccgac caccatctcg tcgggcacga
     3121 acacgtcgtc gaagaacacc tcgttgaaca gctcgtcgcc ggtgatctcg cgcagcggtc
     3181 ggatcgtgat gccctcggac ttcatgtcga cgaggaagta cgtgatgccc ttgtgtttcg
     3241 gagcgtccgg atcggtgcgc gccaggcaca caccccactg cgccttatgc gcggccgacg
     3301 tccacacctt ctgcccggtg agccgccaac cggtcccatt tttgctcctc gcgtccgcac
     3361 cttgagcgcg aacagctttc gtgcgcagcg aggcgagatc ggagcccgcg ccgggttcgc
     3421 tgaacaactg acaccagatc agctcgccgc gcagcgtcgc gggcacgaag cattcgatct
     3481 gtttggggct gccatgctcg aggatcgtcg gcgccgccca ccagccgatg accagatccg
     3541 gccgttcgat ccccgcggcc gccaattcct ggtcgatcag cagctgttcg gccggcgagg
     3601 cctcccggcc gtacggcttc ggccagtgcg gggccagcag ccccgcctcc gccagcgcga
     3661 cctgacgctt gtcggagggc agcgccgcga cgtcgtcgag tgcggcggcg atctcgggcc
     3721 gcaagtggtc gacggactcc aaatcgacgt gcagttcacg gcgcacaccg tcgcgggtca
     3781 gtgccgcgac acggcgcagc caccgggact tgccgccgag gaactgcgaa atagcatgcg
     3841 cccggcgcag atacaggtgc gcgtcgtgct cccaggtgat gccgatacca ccgagcacct
     3901 gaatgcagtc cttcgcgttg gccttcgcgg cctcgatccc gacggctgcg gcgacagcgg
     3961 cggcgatgga aagctgcgaa tcgaagcctt ccgacgcggc gcgtgcggca tcggcggcag
     4021 ccaccgaggc ctgttcggac cgcagcagca tctcggcaca caggtgcttg atcgcctgga
     4081 agctgccgat cggcttgccg aactgctcgc gaactttcgc gtactcggtc gcggtgtcca
     4141 gcatccagcg cgccagtccg gtggcctcgg ccgccaacac ggtggcaacc aggtcttcga
     4201 cccattgcgt cgcgacgtcc ggcgccgcag cgggaacgga atcgagcacg actcgcgcca
     4261 gcggtcggga gaagtccgtc ggcgtcagcg gctcgacagt cgcgccggcg ctgccggtgt
     4321 cgatgagcag ccactgcgac ccggccgggg cgatcacgta ctcgccgggt ctggcgccga
     4381 gcaccaccgg aagggttccg ctgacctggc ccgattcgta gcgcaaatcg ccgtcgagtg
     4441 cgacgccggc gctgcgctgg ccggacatca attcgccgag tacctgttcg tcgtcgagca
     4501 gcagcgtggc caacgcggtg gtggtaaccg gaccgggcac tagtgcgtac gccgcctcct
     4561 cgagcatcgc gcacaggtca gcgacgccgc cgcccgcgcc ggacagcgat tcgggcaccg
     4621 ccacgccgaa gaatcccaac tcagctaatc cgctaaacgg cttcagccag gcgtcggtgt
     4681 cgccttgttc gacggcccgt gcggcagcga tcgcgccgga tgccggcgcc caggagcgga
     4741 ccagctctct ggcggcggtc tgctcgtccg agatgctcgc tgacacgatc agcctcctaa
     4801 gccgagaggg aaaggcacgt gcagcctact agaacgtgtt ctaatagcat gtgtgccggt
     4861 cgtcaaaccg gtacgcgtaa cagcaggaca cgagcgtctg cccacccgcg cagggcgaga
     4921 agtttccggg cggcgtgcgt atcgtttcct caggtacgac gaagagaagg ggccgctcac
     4981 cgcatgtcga cgccagcgca ggccagttcc ggctcgccgg gcgaaccccg tcagccccag
     5041 gttctggcgg tggccgcctt ggccgagtcc gagctcggct cggaggctca acgggagcga
     5101 cgcaagcgca tcctcgacgc cactttggct atcgcctcta agggcggata cgaagcggtt
     5161 cagatgcggg cggtcgccga acgtgccgac gtcgccgtcg gcacgctgta ccggtacttc
     5221 ccgtcgaagg tccatctgtt ggtgtccgca ctcgggcgcg agttcgagcg tatcgacgcc
     5281 aagaccgacc ggatgacgct cggcggcggg acgccgtatc agcggctgca ccagatgatc
     5341 agccggctga accgggcgat gcagcgcaat ccgttgctca ccgaggcgat gacacgcgcg
     5401 ttcgtgttcg ccgacgcgtc ggcggccggc gaggtcgacc acgtcggcaa actgatggac
     5461 agcatgttcg cgcgcgcgat gagcgacggg gagccgacgg aggatcagta ccacatcgcc
     5521 cgggtgatct ccgacgtgtg gctgtccaat ctgctggcgt ggcttacccg ccgcgcgtcg
     5581 gccaccgacg tcagcaagcg actcgatctg gccgtgcgac tgctgatcgg cgacgaagag
     5641 caccccaaga tctgagctag cccggcgctc cggcggtgcg cccgcggacg agttcggtcg
     5701 gcagcacctc gtcgacgggc agcccgcttc tcggcggcgt atgcagcagt tcgcctgcca
     5761 gacggccctt ttcgaggttc ggctgtgtca cggtggacag accgcggccc aacgccgccg
     5821 gcaccccgtc gaagcccgtg accgtcatct ggcccggcac gtagatgccg cgcgcccgca
     5881 gatagtccat cgccgacagc gccaacacat ccgccgtgca catcagcgcg gtgatcccgg
     5941 gattggcttc cagcgccact tcggcggctg ccccacccga cgtcggcaga tgttcgtagc
     6001 attccaccac cgtcagcgag tcgggatcga caccggccgc ctccatcgct tcacgcaccc
     6061 cgctgatgcg ttcgcgctga acgtgaaagt gcgggaatcg caaccgttct gggctggcga
     6121 cggccgccgc cccgggtgtg cgctcccggc cgagccgcat ggtcagcaac ccgatctctc
     6181 gatgcccaag tcccacaacg taatcggcca actcccgcat cgccgcgcgg tcgtcgatac
     6241 agaccctcga cgtgccaggc acgttcttgg gctggtccac gaccaccact ggcagattgc
     6301 gttttagcgc cacctccagg tacgggtcgt cgtcggccac cgagtacacc acgaagccgt
     6361 cgacgccggc gttgagcacc gcgtgtgacc cctcttcgat gctgcggccc ggcccaaccg
     6421 ccagcagcag taggccttga cctacctctt cgcacgattc ggccaccccc gcaacgaagc
     6481 tcaccgcagc gggatcgctg aacgcatagt tcagcggctc ggtaagcacc agaccgacag
     6541 cccccgcctt gcgggtgcgc agcgaccgcg ccaccggatc cggccccgga taaccgagcc
     6601 gcttggccgt ttccagcacc cgctcccgga gatcggccga cagctgatcc ggccggttgt
     6661 aggcgttgga gatcgtcgtc cgggagacct tcagttcggc cgccaacgag gctagggtgg
     6721 cccgccgccg gtgcacggaa ggcctggaca tggtcttcga ggttagtcca tctcgctgcc
     6781 gggaccttcg gcgatgcgct acattaatgg aaacggtttt catttgtgtt aaggcgaggg
     6841 agacatgggt cgggcaatga tatggctggt cgcggccctc gtggcggtgt ccgcctgcgg
     6901 atcgccgccc gaccaggccg acaaagtaag cgtggtcgcg tcgacgagcg tgtggggcgc
     6961 cattgccgag gtggtgggcg gcgactacgt gcaggtccgg tcgatcatcg acagccccgc
     7021 agaagacccc cactcctacg acgtcagccc cgccgacgcg gccgcgatcg ccgacgcaca
     7081 actggtcgtc tacaacggcg gcggctacga caacttcgtc gacgccgtgc tggagcagca
     7141 tgacgcggta aagcgagtcg atgcgttcac ggtcggcggt caccaacagg gaagcaaccc
     7201 gcatgtcttc tacgacctcg gcaccgtgtc gctactggcc ggtgccgtcg ctgaccaact
     7261 cgccgccgtc gaccccaccc acgccaccga ctacaggtcg aacgccgaga agttcacgga
     7321 ccaagtgcac ggcatcgagg ccacccagcg cggcatcgcg gccgaacacg tcggctcggc
     7381 ggcaatcgcc accgaggaca tcgcccagta cctggaggcc gcagttggct tgaccgacag
     7441 gacacctgag gggttcttca aggcggtggc cgcggacttc gacccgggac cggccgatat
     7501 cgcagccgtt ctcgacacgg tgaactcgca cggcgtgcag gtggtgctct tcaaccctca
     7561 gaccgacaca gcagtgactc gtcgcatcgt ggacgccgcg agcacggccg gggtcccggt
     7621 cgtggaggtg cgtgaaacac tgcctgccgc aaccgacttc ctgacctggc aacgtcatac
     7681 cgtcgagcag ttgagcgccg cgctgcaacg agccgaccct cacacgccat gaacgcgcca
     7741 ctcgtcgagc tcagagccgc ccgcctgagc tttggcgacc gcgcgctgtg ggacggtctc
     7801 gacttgaccg tcggcgctgg cgagttcgtc gcgatactcg gaccgaatgg ctgcggcaag
     7861 acgtcgctgt tgcgggtgct gaccggacag cttccgttga cggaaggttc tgcttacatt
     7921 gcggggcgac cggtttcgtc gggccgcagc accgtcggct acattccgca acacggggcc
     7981 tcagaacgcg gcctgacgat gcgcggtggt gatctcgtgc ggctcggtgt cgacgggcac
     8041 aggtggggct tgaccggtcg ccgagcggct cgactacagc gcgacctggc cgtcgaagca
     8101 gcgctgagtc aagtggctgc gacacatctt gcccgcacgt cgctttccgt gatgtccggc
     8161 ggtgagctgc aacggctgcg gatcgcgcag gcactggccg gagatcccgt tctgttgctg
     8221 tgcgacgaac cgctgctgaa tctggatccg gccagcgcgg agcaagtttc ggagcttatc
     8281 gacaagcggc ggcgcgatgc ggggacggcg gttttgttcg tcacccacga gatcaacccg
     8341 gtgctgccct acgtcgaccg ggtgctctac ctggtcgacg gccggttccg gatcggcacc
     8401 gtcgacgagg tgatgacgtc ggagaatctg tccgctctgt accgggccga cgtcgacgtg
     8461 gtgcacgtgc acgggcaata cgtggtcgtg ggaaatcggg gcttctgtca tgagtgacag
     8521 cctcaacagc ctgctgcaca acatgttcgc ttttcacgtc accgcggatc tgttgggccg
     8581 cggtttcgtc cagcaggcga tcgccgcagg tgcgctgctg gcgttggtgt ccggcttgat
     8641 cgggccgttc atcgtgatgc ggcagatgtc gttcgccgtg cacggttcca gcgaattgtc
     8701 cttgaccgga gcggctttcg cgctactcac cgggctcaac gtaggagctg gtgcgctggt
     8761 cggcagcctg atcgcggcgg tgctattcgg catcctgggt cagcgtgcgc gggaacggga
     8821 ttcggcgatc ggcgtcgtcc tggcattcgg gttggggctt gcggtgttgt tcattcacct
     8881 gtatcccggc cgggcgggca cgagtttcgc gctgctgacg ggtcagatcg tcggagtggg
     8941 gtattcgggg ctggcgcagc tggtgggcgt ggcggtcatc gtcgcgattg tgctgactgt
     9001 caccggccgc ccgctgctgt tcgcgacggt cgatccggaa gtcgcgtcgg cgcgcggcgt
     9061 gccggtgcgc gggctcggca tcgtcttcgc ggcgctggtc ggcgtcgtcg ccgcgcaagg
     9121 tgtgcagatc gtcggagcgc tgctggtgat gtcgttgttg atcacgcccg ccgcggccgc
     9181 cgcccggctg ttttcctcgc cgtccgcgac gatagcggcg tcggtgatct tcgccgagtt
     9241 cgcagcggtc ggcggcatcg tgctgtcact agcgcccggc gtgccgatat cggtgttcgt
     9301 aacgatcatc tcgttcgcga tcttcgtggt gtgttggctg gtcgaacgtc gtcgccggcc
     9361 agtactgcac gcttaggctt gccttcgtgt ccgccgacca ccgcacgatc gacctgaacg
     9421 ccgacctcgg ggagggtttc ggggtctggc gtctcggcga cgacgaggag atgctgcagc
     9481 tggtcaccag cgccaatgtg gcatgcggct tccacgccgg cgatccagcc gggctgctgc
     9541 gaacgtgccg cgccgcggcc gctggcggtg tccgcatcgg cgcgcaagtc gcctaccgcg
     9601 acctggccgg cttcggccgg cgctacatcg acatctctgc cgaggacttg accgccgacg
     9661 tgatctatca gatcggcgcc ctgcagggcc tcgcgcatgc ggcgggttcg gtggtgtcct
     9721 atgtcaaacc ccatggcgct gtacaacacc gtggtgacga aggaggaaca ggcgcgtgcg
     9781 gtcgcgcagg cagtacacgc ggtggattca gcgctgccgg tgctgggctt tgcggggtcg
     9841 gccatgttcg acgccgcgac gggagcgggt ttgcgctgtg tcgctgaggc cttcgctgat
     9901 cgggcctatc ggcccgacgg ccaacttgtg tcgcggcgcg aacctggtgc ggtgattcat
     9961 gatccggacg tggtggcgca gcgcgtggcc acgatggtcg cccgcagcga agttaccgcg
    10021 atcgacggtt cggtgattcc cgtcgaggtg gaatcggtct gcgtacacgg cgattcacca
    10081 ggcgcggtga agattgctac cgcggtgtgg gaacgtttga ccgctgacgg cgtcgatttg
    10141 aggccgttca cgtaggcccg acaagccgaa ctttgacgag caagctaatt ttgttcggta
    10201 gcgacgtgca cgacatcggc atcgggtaca gttcgtgtct cgtcgcgagt aggagactgc
    10261 cgctgtcacg ggggtgaggc aaggaggcac cgatgcggtg gaccggcgag aacgacccgg
    10321 tagtcacacc gttcatccgc agtcagttgg cggtcttcct ggcgctgacg gtggtggcgc
    10381 tgggcgccct cgcgttccac tatgtccggc ttccgcagaa ggtgggcatc ggccagtaca
    10441 cgttgtacgt cgatctgccg agagccgggg ggttgtatgc gacgggcgcc gtgacatacc
    10501 ggggcgcgac gataggtcag gtcaccaaag tggagcccac cgagcggggc gcgcgggcga
    10561 cgctcagcat caacaaccgc tacaagatcc cgctggacac gtcggcgaac gtgcactcgg
    10621 tgtcggcagt cggcgagcag tacctggacc tcgtatcacc caccggcgat gcgagccagc
    10681 atttccgcga cgggcagacg atcaccaaca gcacggtgcc gtctgaggtg gggccggcgt
    10741 tggatgccgc caaccaggcg ctggcggcgt tgccgacgga caagatcgac aacctgctca
    10801 ccgaggcgtc gcaggcggtc ggcgggctgg gcccggcgct gcagcggctg gtggattcca
    10861 ccacggcgat cgccagcgat ttccgcgaca acctggcccc ggtcaacgac atcatcgccc
    10921 attcggcgcc gatcctggat tcgcaggtca actccgggga cgcgatcggg gcgtggtcgg
    10981 ccaacctgaa cgtgatcgtg gcgcaggccg ccgagcagga cgcggcgctg cgcagcgggt
    11041 tgcagcaggc ggccccaacg gccgatcagc tcaacgcggt gttcggcgac gtgcgagagg
    11101 cgttgccgca gaccctggcg aacctcgcga tcgtcaccga catgctcaag cgctatcaca
    11161 agggcctcga acaggccctg gtgatcctgc cgcagggagc cacggtcgcc caggcgggca
    11221 ccatcttccc cggcgagggc ctgctgcact tcggcctgtc gatcaaccag ccgccgccgt
    11281 gtctgac
//
LOCUS       DASWBF010000127        13139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_13147, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000127 DASWBF010000000
VERSION     DASWBF010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13147"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VF874_04350"
     CDS             complement(<1..669)
                     /locus_tag="VF874_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX9175345.1"
                     /translation="MAMTVRRLIAVRKLGLSLVAGRAGLDRTITWAHAIELADPSPWL
                     SGGELVMTTGLHFPDTDAGQHEYVQRIVDSGSAALAIDTGVRFQKVPPAVCAAGDEFG
                     IPVLAVAATTPFIAISRAVIDDLTADQVRLVQRVVEGQKNLARAAMHGGIPALVDTLS
                     RTIDSAVCVIDRSGVVLAESGADAVDLVNRVRNQLDRGQRRQRGASRAVVDEYGSLTI
                     QQLPT"
     gene            884..2077
                     /locus_tag="VF874_04355"
     CDS             884..2077
                     /locus_tag="VF874_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9175346.1"
                     /translation="MTATMTPITAGRQRPLEMFGIDTLLDDDERDIAATVRQFVDTRL
                     RPHIADWFESATLPRELAKEFGELGLLGMHLHGYGCAGANAVSYGLACLELEAADSGF
                     RSFVSVQGSLSMFSIDRFGSEEQKQEWLPRLAAGDAIGCFGLTEADFGSNPAGMRTWA
                     RRDGSDWILSGTKMWITNGGLADVATVWARTDEGIRGFLVPTGAPGFTTNTIHRKLSL
                     RASITSELVLDSVRLPASAQLPNAVGLSAPLSCLNEARFGIVFGALGPARDSLETAID
                     YADTRDVFDRPLSSYQITQEKLANMTLELGKGMLLALHLGRLKDSVGLTPDQISLGKL
                     NNVREALVIARECRTILGASGITLEYSPLRHANNLESVLTYEGTSEMHLLSIGRALTG
                     YAAFR"
     gene            3068..3655
                     /locus_tag="VF874_04360"
     CDS             3068..3655
                     /locus_tag="VF874_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175347.1"
                     /translation="MTADVAVLHFDGRKLPQALRGLPIADATDIEAAAVYRRLIVVGS
                     QADLAAVLTTLLRGERLDVEIGFVSGRRGARRAVSGEAQRVPLIRDDTGHVIVGAATW
                     RGVDGPLHGEAVVDDTTLFDGDVSGVRIEPTLTIPGLRAAVLTSRGRRGRWVAGRAAQ
                     LGTTGAFVVRDGVAAPRSVRRSTFYRHTKGWLLVR"
     gene            3753..4532
                     /locus_tag="VF874_04365"
     CDS             3753..4532
                     /locus_tag="VF874_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9175348.1"
                     /translation="MNIRPMRQSVRPSPVFLAIVGITVVGAVMAWLAAEEVTALAYAG
                     VFVFVMAGWVVSLCLHEFGHAYTAYRFGDRDVEVRGYLTLNPLKYSHALLSIALPLVF
                     IALGGIGLPGGAVYVHTRGMTNRQRTIVSLAGPFANVVLAVVLLTLTAAFYDPAHRIF
                     WYGMAFLGFLQVTAVLLNLLPIPGLDGYNALEPHLSYETQRALEPAKQWGFFILFILL
                     IAPPLAIAFFSVVDMVVGLSGVKQTWWENGSALTRFWSAWF"
     gene            4574..5467
                     /locus_tag="VF874_04370"
     CDS             4574..5467
                     /locus_tag="VF874_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9175349.1"
                     /translation="MGAGHDHTGADTRVSRMIIAAAILTGFFFVELTTALLINSIALL
                     ADAGHMLTDLVALFMGLTAVILARRGSNSPARTYGWHRAEVFTAGVNALLLVGVAVFI
                     LYEAYERLGNAPDIPGVPMIVVAVAGLIANAAVVLLLRSSAKESLAVKGAYMEVVADT
                     VGSIGVLIAGVVNVTTHWPYADIIVAVFVALWVLPRAIALARAASRILSESSPDHVDV
                     DELRAALSAVDGVLDVHDLHVWTLVPGKDMATAHLTSCDDSARVLADARAVLAARGLA
                     HATVQVEPPENADDCQGQADW"
     gene            complement(5471..5887)
                     /locus_tag="VF874_04375"
     CDS             complement(5471..5887)
                     /locus_tag="VF874_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HEX9175350.1"
                     /translation="MTQFGDLLGPDPVLLPGDPQAEAELEANENPAIVAAAHPATSVA
                     WAQLAEDALADDNAVTAYAYARTGYHRGLDQLRRNGWKGFGPVPYDHQPNRGFLRCVA
                     ALARAADAIGETDEYNRCLDLLDDCDPGARAKLGVG"
     gene            5999..6853
                     /locus_tag="VF874_04380"
     CDS             5999..6853
                     /locus_tag="VF874_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HEX9175351.1"
                     /translation="MSNPPGPDQDATPTPEDEDVQEPDPSELPTEEAPEPSEAFTEVI
                     SSSDPEAEFESAEPTIVLSGPNQSQQATQPRYTAPSGFDARSTEIIRTPAPEPPTDVM
                     ATSPPPPDPSAAASAVTNPRPVGPQAIPPRPGVRRHRSWGLVVALILLIAALAVIAVL
                     GTIYLTRQASPKVSQEDLVRETIEEFDVAIQEGNLSELRGITCSTTRDRYVNYPDQQW
                     AATHKRVKAAKRYPMVAKIDQVVVNDKHAEANVTTFMAYAPQVQSTRSFDLQYLDGRW
                     KICEGPIS"
     gene            complement(6850..7884)
                     /gene="fbaA"
                     /locus_tag="VF874_04385"
     CDS             complement(6850..7884)
                     /gene="fbaA"
                     /locus_tag="VF874_04385"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX9175352.1"
                     /translation="MPIATPEVYAEMLGRAKEQSFAFPAINCTSSETINAALKGFADA
                     GSDGIIQFSTGGAEFGSGLGIKSMVTGALALAEFAHVVADKYPITVALHTDHCPKDKL
                     DTYVKPLLKVSQQRVAEGRNPVFQSHMWDGSAVPLDENLAIAKELLKEAAAAKIILEI
                     EIGVVGGEEDGVEAEINEKLYTTPEDFEKTIEALGAGEHGKYLLAATFGNVHGVYKPG
                     NVQLRPDILAAGQKVAAAKLGLPDDSKPFDFVFHGGSGSLKSEIEESLRYGVVKMNVD
                     TDTQYAFTRPVAGHMFTNYDGVLKIDGEVGDKKVYDPRSYMKKAEANMSERVVEACND
                     LHSAGRSVTS"
     gene            7967..8638
                     /locus_tag="VF874_04390"
     CDS             7967..8638
                     /locus_tag="VF874_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014943039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX9175353.1"
                     /translation="MSTTLVALPAIMDPWTYIGPDGLFASAILPGILVVVFIETGLLF
                     PFLPGDSLLFTGGLLAAKGTLNIWTLAIAVPLVAIAGDQSAYFIGRRVGPALFTKEDS
                     RFFKKHYVTESHEFFEKYGPFTIILARFVPFARTFVPVIAGVSKMRYPMFLGFDIVGG
                     FAWGAGVTLAGYFLGNIEFVNRHLELIILGIVFVSVLPAVISVGRGLLGRRRAQKDAV
                     PEESG"
     gene            9579..9893
                     /locus_tag="VF874_04395"
     CDS             9579..9893
                     /locus_tag="VF874_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175354.1"
                     /translation="MRSVPTAASSHQRARRVRQACHAAAVASAANGATMLEFQTNVDA
                     LIADGLTAISRPATCPASGPATVLPSHHVAATPPTPTAASVATAATGFASVRNALGAR
                     IQ"
     gene            10216..11616
                     /locus_tag="VF874_04400"
     CDS             10216..11616
                     /locus_tag="VF874_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-6-methoxyphenol hydroxylase-like
                     oxidoreductase"
                     /protein_id="HEX9175355.1"
                     /translation="MAKIGGHAVVLGASMAGLLAARTLADFYENVTIVERDMLPEAAA
                     NRRGVPQGRHVHGLLARGAQALEEMFPGVLDELARDGARLFDGRDLSALYYNINGHQM
                     VRTGSASSFTAYAASRPFLEYHVRRRVSALPNVTLLEDHDVAGLTSTSDHGRITGVRT
                     VDRCSRDESVLAADLVVDATGRGSRMPVWLDELGYGRPVEDHVVVHLSYSTQWLRMPA
                     EALHEMAVLIGVLPGRPTGMGMLGCEKNMWLFTLMGMAGRQPPCTRAEMFDWAEDFAP
                     PHVLDAVRAAEPIGDVARHRFPSSQWRRYDKMRRFPDGLLVTGDAICSFNPIYAQGMT
                     VAALEALALRDCLARGTSDLAPRFFRAAAKPIRQAWQLSAGGDLALPEISGEQSVATR
                     LFNGYVDRILTAAEYDAAAVDQFSRVTALVDPAVRLLRPAMIWRAAAANYRRPRYIAS
                     AQAMWPSKPRDVAMAR"
     gene            complement(11644..12765)
                     /locus_tag="VF874_04405"
     CDS             complement(11644..12765)
                     /locus_tag="VF874_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175356.1"
                     /translation="MLVGAAEGAGSTAWASDLERCLQLGGTDLNDDKLFAALNTLTIY
                     YINRGELRRAEPIVALVEPRRPSIPMGGAWGVMALFGGQLDVARPLIEQWKFDLALAS
                     QEFEDSWIVPFDYRAAACVGLGALRLLQGDLAGADAEFAEAADRSERAAFPKGPNTLA
                     YVRFGQIWMCTESAQLDRAATLAADLLAVTERHGLDGWHLVGVVQHAAVSASISLDSG
                     CDPADVAAQLETASRRLDTWQDAGYGAFRPMYDRVLARLLIAAGHHEEARRRIDTGLR
                     LADATGVHYYDAELLRLRAKLQADPDTKAADLAAAIDLTRRQEAFLFELRSALDDFKL
                     RGEPARCGLADAVKRMPADSAWPELTRARAALGLAESAQ"
     gene            complement(12678..13061)
                     /locus_tag="VF874_04410"
     CDS             complement(12678..13061)
                     /locus_tag="VF874_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175357.1"
                     /translation="MAAELPPPSVRRRLHAKVADALIEGVGDPDWRLVAAHYEQAERY
                     DRAADAYLQASTEAQRRAALAEAREYLTLAIDHLDRVPPSAERNRQEMALRLQRGCSS
                     APRKAPEVPRGPAIWSGACSWAAPT"
BASE COUNT         2185 a         4281 c         4310 g         2363 t
ORIGIN      
        1 ggttggcagc tgctggatgg taagactgcc gtactcgtcc acgaccgcac gactggcccc
       61 tctttgccgt cgctgtcccc gatccagctg gttgcggacg cggttgacca aatcaactgc
      121 gtccgcgcct gactcggcta gcactactcc agagcggtcg atcacgcaga ccgcagagtc
      181 aatcgtgcga ctcaacgtgt ccaccaacgc cggaatgcca ccgtgcatcg ccgcgcgcgc
      241 gaggttcttc tgcccctcta ccacccgctg caccaaccgg acctgatcgg cggtgaggtc
      301 gtcgatcacc gcccgggata tggcgatgaa cggtgtcgtc gcagcgacag ccaacactgg
      361 aatcccgaac tcgtcgccgg ccgcgcagac cgccggtggc accttttgga agcgcacgcc
      421 ggtatcgatg gccaatgccg cacttccgga gtcgacgatg cgttgcacgt actcatgttg
      481 gccggcgtcc gtgtcgggga aatggagacc ggtagtcatc acgagttcgc ccccggacag
      541 ccatggactg ggatcggcga gttcaatcgc gtgggcccag gtgatcgtgc ggtcgaggcc
      601 agcgcgtccc gcgacgagcg acaatccgag cttgcggacc gcaatcagac gccggaccgt
      661 cattgccatg acgaccgctc ccggtgcgca attcgtctat ccaccttgac tccgttggag
      721 gcctcgtcgg taccgggaac gttgagcata gccaaggaat ctgtgtactt atagtcaaga
      781 cttggtcgac aagatcgaca aattggacat ccttgaccaa gcctttcgtc cctatagtcg
      841 aagttgcaga gcaacagcgc tgcctcgata cgaaggacaa cgcatgaccg cgacgatgac
      901 gccgattacg gccggacggc aacggccact ggagatgttc ggcatcgaca cactgttgga
      961 cgacgacgaa cgcgacatcg cggcgacggt acgccaattc gttgacaccc ggttgcggcc
     1021 ccacatcgcc gactggttcg agtcggcgac cctcccccgc gagctggcca aggaatttgg
     1081 cgaacttgga ctgctgggaa tgcacctcca cggctatggt tgcgcgggcg ccaacgcggt
     1141 cagttatggc ctggcgtgcc tcgagctcga ggcggccgac agtggattcc gcagtttcgt
     1201 gtcggtgcag ggatcgctgt cgatgttctc catcgaccgc ttcggttcgg aagaacagaa
     1261 acaggagtgg ttgccgcggc tcgctgctgg cgacgcgatc ggatgtttcg gcctcacgga
     1321 agcggatttc ggttccaatc ccgctgggat gcggacatgg gcccgccgcg acgggtcgga
     1381 ctggatcctc tccggcacca agatgtggat cacaaacggc gggctggccg acgtggccac
     1441 ggtgtgggcg cgaaccgacg agggcatccg cggcttcctg gtaccgaccg gtgcacctgg
     1501 cttcacgacg aacaccattc atcgcaaact gtcgctgcgg gcctcgatca cgtccgaact
     1561 tgtactcgac agtgtccgtc tgcccgcctc tgctcaattg ccgaatgcgg tagggctgag
     1621 cgcaccgctc tcgtgcctga atgaggcgcg tttcggcatt gtgttcggcg cactcggccc
     1681 cgcaagggac agcctagaaa ccgcaatcga ctacgccgac acccgcgatg tcttcgatcg
     1741 cccattgtcg agctatcaga tcacccaaga gaagctcgcg aacatgacgc tggaactcgg
     1801 caagggaatg ctcttggcgt tgcatctcgg tcggctcaag gactcggttg gactcacgcc
     1861 ggaccaaatc agcctgggca agctcaacaa tgtgcgcgaa gcgctggtta tagcgcggga
     1921 atgtcggacg attctgggcg ccagcggaat caccctggag tattcgccgc ttcgccacgc
     1981 gaacaatctg gaatcggtct taacgtatga aggcactagc gaaatgcatc tgctttccat
     2041 cggcagggcc ctgaccggat atgccgcgtt tcgctgacgt tgacgatccg agggcgaaaa
     2101 gccggaatca cgtgtagacg gcctgtactc tggcgaccgt tttctgctcg gtgccggctc
     2161 gcaagtgaaa gcccatatca ccaaacggtt ttcgatgccc ggtgggcgcc tgcggaacgc
     2221 atgcgggccc tagcgatgct aataggagta gtggggcatt cccgggcgag gagaggccgg
     2281 agcggtactc gtagtcgtcg tcgtagtgac gggagccgca gccggagtcg gagctggagc
     2341 ggggacaggt gcctgatcgc cggaaggggt aggcagcagg ccgagcgcgc ctgcgaaaag
     2401 ccctacagct gctgcagcaa gtaaccagcg gagtcgcccg tgtctggtat tcatctgact
     2461 ctgagccctt cgattgagtg gcggatgtcg gtgtgcaccg tgcgcgcacc catacaccgt
     2521 gtaaacgggg gtagccaacg cagtgttcaa ctacaggcgc gcccggctga aggactggta
     2581 gcggttcgaa ctcgctgtgc accccgagcg ccttgatcgc cgggtgcttg ggggtctaag
     2641 gacgcgtgcc acgcgggcac cgctgcgccg gtcaggtgcg ccagcggcac cgttcccagc
     2701 gtcgggttga tgtgttgttc gagcagcgcc gggtattgca atctggtgtg ctctttgacg
     2761 tcgcagtgtt ccaaccacac cgcggcgtaa gcggctggcg tgatcgcctt ggcgttatgc
     2821 tccgcggcgc cagccgtggg cgtgctccag tcgccgtttc gatcagccgg cgctcctcgg
     2881 ccaaccaaca ctcagctgca gggcaccgac tacgtcgtgg gcgcggtatg acaggtcaga
     2941 tccggcccga catacgatgc ctggaaccgt gcccccaacc gcggtgcccg ttcttgcctg
     3001 ccattacccg ctggtagtgg tgcagccggt gacccacgct atccgaggtt tgctcaggag
     3061 tctcgctttg accgccgacg tcgccgtgct gcacttcgac ggccggaaac tgccccaggc
     3121 gctgcgcggg ctgccgatcg ccgacgcgac cgacatcgag gcggccgcgg tgtaccgccg
     3181 actgatcgtc gtcgggtcgc aggcggacct ggccgcggta ctgacgacgc tgttgcgggg
     3241 cgaaagactc gacgtggaaa tcggtttcgt gtccggccgc cgcggcgcgc gccgggcggt
     3301 ctcaggtgag gcgcaacggg tgccgttgat ccgcgacgac accggccacg tgatcgtcgg
     3361 cgcggcgacg tggcgcggcg tcgacggccc gttgcacggt gaggcggtgg tcgacgacac
     3421 caccctgttc gacggcgacg tgtccggggt ccggatcgag cccacgctca cgattcccgg
     3481 gttgcgggcc gcggtgctga cgagccgtgg gcggcggggc cgctgggtgg cgggccgcgc
     3541 cgcgcagctc ggcacgacgg gtgccttcgt cgtccgcgac ggtgtcgccg cgccccgctc
     3601 ggtgcggcga tcgacgttct atcggcacac caagggctgg ctgctggtgc ggtgaccgtc
     3661 cgcgaaacta acgttttgca ggccgcctcg cacttcctct gcaaaacgtt gatctcgtgg
     3721 ccggttgcac tccctcccgg tactttcgtg tggtgaacat ccgtccgatg cgccagtcgg
     3781 tgcgtcccag ccccgtcttc ttggcaatcg ttgggatcac cgtcgtcggc gctgtcatgg
     3841 cctggctggc cgctgaggag gtgacggctc tcgcgtatgc aggcgtattc gtgtttgtca
     3901 tggccggctg ggtggtgtcg ctatgcctgc acgagttcgg ccacgcctac accgcgtacc
     3961 ggttcggcga ccgcgatgtc gaggtgcgcg gttatctcac gctgaacccg ctcaagtact
     4021 cccacgcgct gctgtcgatc gcgctgccgc tggtcttcat cgcgctgggc gggatcggcc
     4081 tgccaggcgg tgcggtctac gttcacacga gggggatgac caaccgacag cggacgatcg
     4141 tcagtctggc cgggccgttc gcgaatgtgg tgctcgccgt ggtcctgctg acattgacag
     4201 ccgccttcta tgaccccgcc caccgaatct tttggtacgg catggctttt ctcggcttcc
     4261 tacaggtgac cgcggtgctg ctgaacctgc taccgattcc gggcctcgac gggtacaacg
     4321 cactcgaacc gcatttgagc tacgagactc agcgtgcgtt ggagccggcc aagcagtggg
     4381 gattcttcat cttgttcatc ttgctgatcg cgccaccgct ggctatcgcc ttcttctcgg
     4441 tcgtggacat ggtcgtcggc ctttccggcg taaagcagac gtggtgggag aacggcagcg
     4501 cactcacccg gttctggtcg gcctggttct gacgcccttg cgacatatgc gtttctgcgc
     4561 atagatttca ggcatgggcg cgggccacga tcacaccggg gccgacaccc gcgtatcccg
     4621 gatgatcatc gccgcggcga tactgaccgg gttctttttc gtcgagctga cgacggcgct
     4681 gctgatcaac tcgatcgcgc tgctggctga cgccgggcac atgctgaccg acctggtcgc
     4741 gctgttcatg ggcctcactg cggtgatact cgcccgccgt ggcagcaact cgccggcccg
     4801 cacgtacggc tggcatcgcg cggaggtgtt caccgccgga gtcaacgcgt tgctgctggt
     4861 gggcgtagcc gtgttcatcc tgtacgaggc gtatgagcgg ctcggcaatg cgccggatat
     4921 accgggcgtt ccgatgattg tcgtcgcggt ggccgggctg atcgccaacg cggccgtcgt
     4981 gcttctgctg cggtcgagcg ccaaagagag cttggccgtt aaaggtgcct acatggaggt
     5041 cgtcgccgac acagtcggca gcatcggcgt gctgatcgcc ggcgtggtga acgtgaccac
     5101 gcactggccg tacgccgaca tcatcgtcgc ggtgttcgtc gcgctatggg tgttgccgcg
     5161 cgcgatcgcg ctggcccgcg ctgcgagccg gatcctgtcc gagtcgtcgc ccgaccacgt
     5221 cgacgtcgac gagctgcgcg ccgccctgag tgctgtcgac ggtgtcctgg acgtgcacga
     5281 tctgcatgtc tggacgctgg tgccgggcaa ggacatggcc accgcgcatc tgaccagctg
     5341 cgatgactcg gcgcgcgtgc ttgccgacgc gcgtgcggtg ctggcggcgc gtggactggc
     5401 gcacgcgacg gtgcaggttg agccgcccga gaacgccgac gactgccagg gccaggccga
     5461 ctggtagacc tcagccgacg cccagcttgg cgcgggcgcc gggatcgcag tcgtcgagca
     5521 ggtcgaggca cctgttgtac tcgtcggttt ccccgatcgc gtcagccgcc cgcgccagcg
     5581 cggccacgca gcgcagaaac cctcgattgg gttggtggtc gtacgggacg gggccgaatc
     5641 ccttccagcc gttgcggcgc agctggtcca agccccggtg gtagccggtg cgggcgtacg
     5701 cgtacgccgt gaccgcgttg tcgtcggcca acgcgtcctc ggccagctgc gcccacgcca
     5761 ccgacgtggc ggggtgggcg gcggcaacga tcgccgggtt ttcgttggcc tccagctcgg
     5821 cctcggcctg cgggtcaccg ggcagaagga ccgggtcggg ccccaacaag tcgccgaact
     5881 gagtcatggc cactattgtg ccgtgcggtg ctgacgcgcg cctcggcgtc accggcgcga
     5941 cagccatgag tcgttaagct tccacgaaga cgtcacgaaa cagaggacac taacgcccat
     6001 gtcgaaccca ccgggtcccg accaggacgc cacgccgacg cccgaggacg aggacgtcca
     6061 ggagccggac ccatccgaac tccccaccga ggaggcgccg gaaccatccg aagccttcac
     6121 cgaggtgatc agctccagcg atcctgaagc ggagttcgag tccgccgagc ccaccatcgt
     6181 gttgtccgga cccaaccaga gtcagcaagc gacccagccg cggtacaccg caccgtctgg
     6241 gtttgacgca aggtccaccg agatcatcag gaccccagca cctgaacccc ctaccgacgt
     6301 gatggcaact tcgccgccac cgcccgaccc gtccgccgcc gcgtcggccg tcactaaccc
     6361 gagacccgtt gggccgcaag ccattccgcc acggcccggg gtgcgccggc accgcagctg
     6421 gggtttggtg gtggcgttga tcttgctgat cgccgcactc gctgtgatcg ctgtgctggg
     6481 cacgatctac ctcacccgcc aggcatcgcc gaaggtttcg caagaggacc tggtgcggga
     6541 gacgatcgag gagttcgatg tggcgatcca ggagggaaat ctgtcagagc tgcgcggcat
     6601 cacctgcagc acgacccgcg accgctacgt caattacccc gatcagcagt gggcggccac
     6661 tcataagcga gtgaaggcgg ccaagaggta tcccatggtg gccaagatcg accaggtggt
     6721 ggtcaacgac aagcatgcgg aggccaacgt caccacgttc atggcgtacg ccccgcaagt
     6781 ccagtcgacc cgcagcttcg acctgcagta cctcgacggg cgctggaaaa tctgcgaggg
     6841 gccgatcagc taggaggtca ctgaccgccc ggcgctgtgc aggtcgttac aggcctcgac
     6901 gacacgctcg ctcatgttgg cttcggcctt cttcatgtag ctgcgcgggt cgtacacctt
     6961 cttgtcgcca acctcgccgt cgatcttcag caccccgtcg tagttggtga acatgtggcc
     7021 ggcgaccggc cgagtgaacg cgtactgggt gtcggtgtcg acgttcatct tcacgacgcc
     7081 gtagcgcaat gactcctcga tctccgactt cagcgaccct gagccgccgt ggaagacgaa
     7141 gtcgaacggc ttgctgtcgt cgggcagccc cagcttggcg gcggccacct tctggccggc
     7201 cgcgaggatg tcgggacgaa gctggacgtt gccgggcttg tagacgccgt ggacgttgcc
     7261 gaacgtcgcc gccagcaggt acttgccgtg ctcgcccgcg ccgagcgcct cgatcgtctt
     7321 ctcgaagtct tccggcgtgg tgtacagctt ctcgttgatc tctgcctcga cgccgtcttc
     7381 ctcgccgccg accaccccga tctcgatctc gaggatgatc ttggcggcgg ccgcctcctt
     7441 cagcagctct ttggcgatgg ccaggttctc gtccagcgga accgccgagc cgtcccacat
     7501 gtgcgactgg aacaccgggt tgcggccctc ggcgacccgc tgctgggaga ccttgagcag
     7561 cggcttcacg taggtgtcga gcttgtcctt cgggcagtgg tcggtgtgca gcgccaccgt
     7621 gatcgggtat ttgtcggcga cgacgtgcgc gaactcggcg agcgcgagtg cgccggtgac
     7681 catgctcttg atccccagcc cggagccgaa ctccgcgccg ccggtggaga actggatgat
     7741 gccgtcgctg ccggcgtccg cgaagccctt caacgcggcg ttgatggttt ccgatgacgt
     7801 gcagttgatc gccggaaacg cgaacgactg ctccttggca cggcccagca tctcggcgta
     7861 gacctcgggc gtggcgatgg gcacgggagt tccttccggc tggtcactgg gctgcgggaa
     7921 tgtccctgaa gtatctcacc aggaggcgcc ggtatgttgg gtcgtcatga gcacgacgct
     7981 cgtggcgctg ccggccatca tggacccgtg gacgtatatc ggccctgacg ggttgttcgc
     8041 gtccgccatc ctgcccggca tcctggtcgt cgtcttcatc gagacgggcc tgctcttccc
     8101 gttcctgccg ggcgattcgc tgttgttcac cggcggcctg ctggccgcca aaggcactct
     8161 caacatctgg acgctggcga tcgcggtacc gcttgtcgcg atcgccggtg atcagagcgc
     8221 gtacttcatc ggccgccggg tcgggccggc gttgttcacc aaagaggact cgcgcttctt
     8281 caagaagcac tatgtgaccg agtcgcacga gttcttcgag aagtacgggc cgttcacgat
     8341 catcctggcg cgcttcgtgc cgttcgcgcg gacgttcgtt ccggtcatcg cgggtgtgtc
     8401 caaaatgcgc tatccgatgt tcttgggttt cgacatcgtc ggcggtttcg cctggggcgc
     8461 cggggtgacc ctggccggtt acttcctcgg caacatcgag ttcgtcaaca ggcatctcga
     8521 gctgatcatc ctgggaatcg tgttcgtgtc ggtcctgccg gcggtcatct cggtgggacg
     8581 cgggctgctg ggacggcgac gtgcacagaa ggacgcggta cccgaagagt cgggctgatc
     8641 ggttttcagg tccgcgcgaa caggcagacc actcggcgcg ggccgtcttc gaagcggccg
     8701 acaaaccgtg agaagcctgc ttgccgtgcg atcgccgact cggccgggcc accacagagc
     8761 attccgacgc tgcgctcgaa gagtcggtcg cacgtgacga cgaggattgc ggccgtcgag
     8821 gtgttcggct tgccgtcacg ccacagcgga aggccatcgc tgcaccgctt ttagccacac
     8881 ccgtgactgc gatcggttga tcgccggcga cagcacggat ttgtgctgcc gtctctgcgg
     8941 ctttcggcca gccgccgtcg ggcgaatcga gcggcggcat cgaagtcagg gacagggcaa
     9001 cgcaggcgac gacaatcgcg atcgccgacg ttctcgctgg ccgtcctgct gcgcttagcc
     9061 gcgccacgcc gacgccgatg acggcgaaaa ctattggggt gagccaggag tggtactggt
     9121 cgttgggcag accgggtgtg aactccgcga gcgtaggtgc cagaaatgta aatgtgagta
     9181 ccgcccagac gatcgtcgca accgcccacc gaccgaattg tgtcgcgacg cctggtcttc
     9241 cggttgccgc gacgaccagt gccgcggcgg cgataatcgc ggcgaatagc ccgcccagca
     9301 cggcgaaagc cacgtccccg gagataggcc acacctcgat ccgccacaga atgatcagcg
     9361 gacgcaggat caacggcggc cctccggacg tttcgggaaa tccgttgcgc agttcgtgaa
     9421 tcaacgttgg cgcgtatccc gcggcgacga tggccgccgc acccagcgcc ggcccgagca
     9481 ttttccgccg ctctgcgcgt gggcgtcgga tgacgtcggc gaccagcagc gcggccagcg
     9541 ccggtaccgc gattacggcc agtaagtggc catgcagcat gagaagtgtg ccgaccgcgg
     9601 ccagcagcca ccagcgggcc aggcgcgtcc gccaggcatg ccatgcggcg gcggtggcga
     9661 gcgcggcgaa cggggcgacg atgttggagt tccagacgaa cgtcgacgcg ctgatcgccg
     9721 acgggctcac cgccatcagc agaccggcca cgtgcccggc gagcggcccg gccacggttc
     9781 tgccgagcca ccatgttgcg gcgacgccgc cgacgcctac tgccgcgagc gtggccactg
     9841 ccgcaacggg attcgcgtcg gtgagaaacg cactgggcgc gaggatccaa taaaagccga
     9901 ccccgtgatg gacgcccggg atcgagctga tcggaccctg cagcggcacg tcgccgtcac
     9961 ggacccacaa cagcatcgtg agcatctgat agccctggtc gtcgtcccac gctccgcggc
    10021 caggcagccc ggcgaagcga aggaccgtcg ccaagaccaa tatcgcggaa aggatcaggc
    10081 cgtcccgccg ggccggcgtc atcgccatcg cgccattatc gcgatggcat tggagtagct
    10141 gactacgcta tccggcgcgc ctgccgccca cgaacgtcgt ccttgtcaca accacgcgac
    10201 agaggtgggt gcgcaatggc gaaaatcggt ggccacgcag tggtcttggg agcgagcatg
    10261 gcgggcctgc tggccgcgcg gacgttggcc gacttctacg agaacgtcac catcgtggag
    10321 cgcgacatgc tgcctgaggc tgctgctaac cggcgagggg tgccgcaggg gcgtcacgtt
    10381 cacgggctgc tggcacgagg cgctcaggcg ctggaggaaa tgttccccgg cgtgctcgac
    10441 gaactcgccc gcgacggcgc acgactcttc gacggccgtg acctctcggc gttgtactac
    10501 aacatcaacg ggcatcagat ggtgcgaacg ggttcggctt caagcttcac tgcgtatgcg
    10561 gcaagcagac ctttcttgga gtaccacgtc aggcggcggg tgagcgcgct ccccaacgtc
    10621 acactgctcg aagaccatga tgtcgccggc ctgacgtcga catcggacca cggccggatc
    10681 accggagtcc ggacggtgga ccggtgcagt cgggacgagt cggtgcttgc ggccgacctt
    10741 gtcgtagatg cgacgggtcg tggctcacgc atgccggtat ggctggacga gttgggctac
    10801 ggacgtccgg tcgaggacca cgtcgtcgtg cacctcagct attcgacgca gtggctgcgg
    10861 atgcctgccg aagcgctgca cgaaatggcc gttctgatcg gcgtgctgcc aggccggccc
    10921 accggcatgg gcatgctggg atgcgagaag aacatgtggt tattcaccct gatgggcatg
    10981 gcaggacggc agccgccctg tactcgagcc gaaatgttcg actgggctga ggatttcgcg
    11041 cccccgcatg tgctggatgc agtccgggcc gccgaaccga tcggtgacgt ggcacgccac
    11101 cgcttcccgt ccagccagtg gcgccgctac gacaagatgc ggcggttccc tgatgggcta
    11161 ttggtgaccg gcgatgcgat ctgcagcttc aacccgatct acgcgcaggg catgacagtc
    11221 gccgcgctgg aggcattggc attgcgtgac tgcctcgccc gtggcaccag cgacctggca
    11281 ccgcgattct tccgcgccgc ggccaagccg atacggcagg cgtggcaact atctgcgggc
    11341 ggcgacctcg cgctgcctga gatcagtggt gagcaatcag tggctacaag gcttttcaac
    11401 gggtacgtcg atcgcatcct gaccgccgcc gaatacgacg ccgccgcagt ggatcagttc
    11461 agccgagtca ccgcgctcgt cgacccggcg gtccggctgc ttcgcccggc gatgatctgg
    11521 cgtgctgccg ccgcgaacta tcggcgaccg cgctacatcg cctcggccca ggccatgtgg
    11581 ccttcgaagc cacgcgatgt cgccatggcg cggtagcgaa tgctcgacag gcagtcgtca
    11641 tcgtcattgc gccgactccg ctaaccccag tgccgctcgg gcccgcgtca gttccggcca
    11701 tgcgctgtcg gccggcatac gcttcaccgc atcggcgagt ccacagcgtg ccggctcacc
    11761 gcgaagcttg aaatcgtcca gcgccgagcg tagttcgaat agaaacgcct cctgccgacg
    11821 agtcagatcg atggcagctg ccaagtccgc tgccttcgtg tctggatcgg cttggagctt
    11881 tgcgcggagc cgcagtagtt cggcgtcgta gtagtgcacg cccgtcgcgt cggccagccg
    11941 aagcccggtg tcgatgcggc gacgtgcttc ctcgtggtgt ccggcggcaa tcaacagtcg
    12001 cgccaacacc ctgtcgtaca tcggccggaa ggcgccgtaa ccggcgtcct gccaggtgtc
    12061 gagacgccga ctggcggtct caagttgagc ggcgacgtcc gctggatcgc agccgctgtc
    12121 caacgagatc gacgcgctga cagctgcatg ttgcaccaca ccgacaagat gccagccgtc
    12181 gagcccgtgt cgttcggtca cggcgagcag gtcggcggcg agtgttgcgg cacggtcgag
    12241 ttgagcgctc tccgtgcaca tccagatctg cccaaagcgg acataggcga gcgtgttggg
    12301 acctttcgga aatgcggccc gctcggaacg gtcggcggcc tctgcgaatt cggcatcggc
    12361 gccagccaga tcgccctgca atagccgcag cgccccgagc ccaacacacg ccgctgcacg
    12421 ataatcgaat ggcacaatcc agctgtcttc gaactcctga gatgccagcg ccaggtcaaa
    12481 cttccattgc tcgatcagcg gacgtgcaac atcgagctgg ccgccaaaca acgccatgac
    12541 gccccatgcg ccgcccatcg gaatagacgg ccgccgtggt tcgacgaggg cgacaattgg
    12601 ctctgcccgg cgcagctcgc cccggttgat gtagtagatc gtgagcgtgt tcagcgccgc
    12661 gaacagtttg tcgtcgttca ggtcggtgcc gcccaactgc aggcaccgct ccagatcgct
    12721 ggcccacgcg gtacttccgg cgccttccgc ggcgccgacg agcatccccg ctgcagacgc
    12781 agggccatct cctggcggtt ccgttcggca cttggcggta cccggtcgag atggtcgatg
    12841 gccagcgtca agtattcacg cgcttccgcc aaagcagctc ggcgttgggc ctccgtggac
    12901 gcctgcagat atgcgtcagc cgcacggtcg tatcgctcgg cttgttcgta gtgggctgcc
    12961 accagccgcc agtccggatc accgacgcct tcgatgagcg cgtcggcaac tttcgcgtgc
    13021 agtcggcgcc ggacgcttgg cggcggcaat tcagcggcca cctctcgcag cagttcgtcg
    13081 cggaaccgct aggaggtcgc tgaataatcg gcgcgcctcc ccggaatatc gctctgaac
//
LOCUS       DASWBF010000128         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_934337,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000128 DASWBF010000000
VERSION     DASWBF010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934337"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF874_04415"
     CDS             complement(<1..284)
                     /locus_tag="VF874_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175358.1"
                     /translation="MIDSHAFSTPRHAMSFPATAKTLQDSAQASNLAALLCPERERKV
                     HVGVLSDCDAARKMKVLWPFEVALRGEASNHLMLRWLETVVVSDEEKAPVR"
     gene            318..1550
                     /locus_tag="VF874_04420"
     CDS             318..1550
                     /locus_tag="VF874_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX9175359.1"
                     /translation="MGSAPASGRLTDHLAIGVLTGLIHRDIVDDVIRECGKREKRSRL
                     LPAHVVMYYVLALNLFFGEAYEEVMRQLVNGLRFLGNWRPNWEVPSTSAISQARTRLG
                     EAPMKLLFERIAVPMARAGTRGSWLRGRRVMAIDGLVLDVPATPDNDKEFGRSGNATA
                     PSPFPQIRLVAVGECGTHGIVDAAFGPVGTGEQTLAAQLIARFTPDMLVLADRNFYSY
                     QAWQQAVATEAALLWRVSASLQLPVLAWLPDGSFRSVLINPAVRGRRREQLIAAAAAA
                     AELDPAQAMPVRVVEYMIDNRAGAGELFCLITTLTDHEFATAVELAAAYAQRWEIELS
                     FDEIEIHQTGGDRVLRSRTPELVKQEIWSLLLTHYAIRHVMKDAADTVGTDPDDLSFI
                     RSYRAIRRQVPNQAGFSP"
     gene            complement(1864..>2145)
                     /locus_tag="VF874_04425"
     CDS             complement(1864..>2145)
                     /locus_tag="VF874_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175360.1"
                     /translation="GSNSSSMTPIVGDHHTRCGPPRTVAAAATSGLADFEIADGWSIW
                     FRFGPSVLLLVAACGPPDGRASRRGRRRRQHGALLGLLRCRRERAPRAA"
BASE COUNT          384 a          721 c          695 g          345 t
ORIGIN      
        1 cgaacgggcg ccttttcctc atcgctcacc acgacggtct ccagccaacg cagcatgagg
       61 tggtttgaag cctccccccg cagggcgact tcgaagggcc acaaaacctt catcttccgt
      121 gcagcatcgc aatcagaaag aactcctaca tgcaccttcc tctcgcgttc gggacacaac
      181 aatgccgcta agttagaggc ttgagccgag tcctgcagcg tcttcgcggt tgcaggaaat
      241 gacatggcgt gtcgcggggt cgaaaaggcg tgggaatcga tcacaccgat attctttgtg
      301 atgtggcgaa tggtgcggtg ggttcagctc cggcgtcggg tcggttgacc gaccatctgg
      361 ccatcggggt gttgaccggc ctgatccatc gggacattgt cgacgatgtc atccgtgaat
      421 gcgggaaacg cgagaaacgg tcacggctac tgcctgcgca cgtagtgatg tactacgtgt
      481 tggcgttgaa cctgttcttc ggtgaggcct acgaagaggt gatgcgccaa ctcgtgaacg
      541 ggctgcggtt tctggggaac tggcgcccca actgggaagt gccgtccacg agcgcgatct
      601 cccaggcccg cacgcgcctg ggagaggccc cgatgaagct gctgttcgag cggatcgcgg
      661 tgccgatggc ccgggccggc acccgcgggt cgtggttgcg cgggcgacgg gtgatggcca
      721 tcgacgggct ggtgctcgac gtgcccgcca ccccggacaa cgacaaggag ttcggccgct
      781 ccggcaacgc caccgcgccc agcccgttcc cccagatccg gctggtcgcg gtgggcgagt
      841 gcggcaccca cggaatcgtc gacgccgcgt tcggcccggt cggcaccggt gagcagaccc
      901 tggccgcgca gctgatcgcc cggttcaccc ccgacatgct ggtgctcgcc gaccggaatt
      961 tctacagcta ccaggcctgg cagcaggcgg tggcgaccga ggcggcgctg ctgtggcggg
     1021 tctcggcgag cctgcagctg ccggtgctgg cgtggctgcc cgacggctcc ttccgctcgg
     1081 tgctgatcaa cccggcggtc cgtggacggc gccgcgagca gctcatcgcc gccgcggcgg
     1141 ccgcggcgga gctggacccg gcccaggcca tgccggtccg ggtcgtcgag tacatgatcg
     1201 acaatcgggc cggtgccggg gagctgttct gcctgatcac cacgctcacc gatcacgagt
     1261 tcgccaccgc agtcgaactc gccgcggcct acgcgcaacg ctgggagatc gaactgtcct
     1321 tcgacgaaat cgaaatccac caaaccgggg gtgatcgggt gctccgatcc cgcaccccgg
     1381 aactggtgaa acaagaaatc tggtcgctgc tgctgacaca ctatgcgata cgccacgtca
     1441 tgaaagacgc cgccgacacc gtcggaaccg atcccgacga cctgtcattc atacgcagct
     1501 accgcgcgat ccgccgccag gttcccaacc aggcgggctt ttccccctga ccgactcacc
     1561 gacgcactca acgaaagcct cgacgagatc gtcgcacaac gcctcggccc acggcgacaa
     1621 cgaacatgtc cccgggtggt caagagatac cgcgcgcaca gtcaccgcat caaacgagca
     1681 accgaccgaa tccaactcca ccccgactca ccgaagatcc acatcttcgc ggcgccgtgc
     1741 ctaacttagc ggcattgggt gttcgggacg ccttcgtgct cggcgaactc gacgcaatcc
     1801 ttgatgtcga acgtcgccat cgggagttcg cgggcgaggc aacaggcggc gtcgcagccg
     1861 tcgttaagcg gcccgcggtg cgcgctcgcg gcggcaccgg agcagtccga gcagtgcccc
     1921 gtgctggcgt cgccggcgtc ctcgccgcga agcgcgaccg tcgggcggtc cgcaggccgc
     1981 cactagcaac agcaccgacg gcccgaaacg gaaccagatc gaccagccgt cggcgatctc
     2041 gaagtctgcg agtccagagg tagccgcggc ggccacggta cgaggcgggc cgcatcgggt
     2101 gtggtggtcg ccgacgatcg gggtcatcga ggagctgttc gagcc
//
LOCUS       DASWBF010000129         3138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_536424,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000129 DASWBF010000000
VERSION     DASWBF010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536424"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VF874_04430"
     CDS             complement(<1..226)
                     /locus_tag="VF874_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEX9175361.1"
                     /translation="MPAALAMALGYHGRHVSLAEMHEATGTGRDGTNALQIAEAAAAY
                     GLRARGGHRARRSAGTTPRHRIALGRSAFRG"
     gene            complement(281..721)
                     /locus_tag="VF874_04435"
     CDS             complement(281..721)
                     /locus_tag="VF874_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175362.1"
                     /translation="MTTDGEVRYESVFRERWRDRRSAGARPGELIKQPGWIDAGLVGL
                     GVLLAAGAVAAGTVTIARTEALPAVAQGTSVTAVRTGPATPDLGTAVQFRDASGTTLD
                     AVVVEVTPTEVTAQLEQPGPESAGELLIPAGRQRLINVLLPRLW"
     gene            complement(841..1050)
                     /locus_tag="VF874_04440"
     CDS             complement(841..1050)
                     /locus_tag="VF874_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175363.1"
                     /translation="MNTNRNKLALRRQSIRTLTADELRIAHGGKGNGTGNTTVTSGSP
                     QTGTTVKTGTCIPTRPTTPPPTQTK"
     gene            complement(1236..1901)
                     /locus_tag="VF874_04445"
     CDS             complement(1236..1901)
                     /locus_tag="VF874_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HEX9175364.1"
                     /translation="MTLSEQIDDGTRLRAAPLRAVAEPPSLPASQGGWKTLLADPPWR
                     FTNRTGKVAPEHRRLDRYSTLDVDAICDIPVADVAAPNSHLYLWVPNALLPEGLRVMN
                     AWGFRYVSNVIWAKRRQDGGPDGRGVGFYFRNVTELILFGVRGSMRTLPHARSQVNMI
                     ETRKREHSRKPDEQYPFIEACSPGPYLELFARHPQPGWAAWGDEAAEDVTPRGRVHKG
                     YTG"
     gene            complement(2015..2710)
                     /locus_tag="VF874_04450"
     CDS             complement(2015..2710)
                     /locus_tag="VF874_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175365.1"
                     /translation="MTDDNDIEHWDAAYVLGALSPQERIEYEGYLAANPERAAALSEF
                     AGMPSILNVLSPEEAVALDATPNGKDDQQPLNLMPSLARAAQRRQRRSRRNVMALVAA
                     TAAAFLAVGVIVTASVFGRPQPSTAPALQAMAPAGRGGITAELGVTEKQWGTRLDWNC
                     EYTKDWSRNVGSYDLVVTTVDGKHTTVGSWRPAGDEASGLAAATVIPTAQIRSVDIRV
                     TGSDAPLAVTTLQ"
     gene            complement(2707..>3138)
                     /locus_tag="VF874_04455"
     CDS             complement(2707..>3138)
                     /locus_tag="VF874_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9175366.1"
                     /translation="LTNGDTQFAEDVVQESLLRLWKKPDVLEQPSESARAWLFTVARN
                     IVIDDRRSSRYSREFSTETLPERNLADAINPAFDKWILSDALLSLSKEHRTAIVRAYY
                     LGQTVADIAEHEQIAVGTVKSRLHYALKALRNALQERGVVR"
BASE COUNT          484 a         1025 c         1036 g          593 t
ORIGIN      
        1 gaccacgaaa tgcgcttcgc cccaatgcaa tacggtgccg cggggtagta cccgcagatc
       61 gtcgagctcg gtggccaccg cgggcgcgca atccgtaggc tgcggcagct tcggcgatct
      121 gcagcgcgtt ggtgccgtcg cgcccggtgc cggtggcctc gtgcatctcg gccagcgaca
      181 cgtggcggcc gtggtagccg agcgccatgg ccagtgcggc gggcaccgca gtcggccatc
      241 tcagcctgcg cgacgaacgg gatcgcgcgc cggtgccacc tcaccagagc ctcggcagca
      301 acacgttgat caaccgctgg cgtccggccg ggatcagcag ctcacctgcc gactccgggc
      361 cgggctgctc caactgggcc gtcacctcgg tcggcgtgac ctcgacgacg accgcgtcga
      421 gcgtcgtccc ggatgcgtcc cggaattgca cggcggtccc caggtcgggc gtcgccgggc
      481 cggtgcggac ggcggtcacc gacgtgccct gagcgaccgc cggcagcgcc tcggtgcgtg
      541 cgatggtcac cgttccggcc gccacagcgc ccgcggccag cagcactccc agcccgacaa
      601 gtccggcgtc tatccacccc ggctgcttga tcagttcgcc ggggcgggct cccgcggacc
      661 ggcggtcgcg ccagcgctca cgaaacacgg actcgtaacg gacctcgcca tcggtcgtca
      721 cgacgacctc actctcggac agccggttct gcaggaattg acgaaggcct tcaacgcgat
      781 tcctccgcac acattgtggc tgctgccaag ccgaggcgcg gccggatcgg cgattggcct
      841 ctacttcgtc tgcgtcgggg gcggcgtggt gggccgtgtg ggaatgcagg tgccggtctt
      901 gaccgtggtc ccggtttggg ggctgccaga ggtgacggtg gtgttgccgg tgccgttgcc
      961 cttgccgccg tgggcgatgc gcagctcgtc ggcggtcagc gtgcggatgc tctgccggcg
     1021 cagcgccagc ttgttgcggt tggtgttcat tggtctctcc ttacctgtgt cgacgcagcc
     1081 cggtggccgc gctcacgggt aggagcacca actcccgcca aacgggacac tctttgagaa
     1141 gtggtgtgct accggctgcg gagttccgaa ccgatccgct ctgccgtggc ggggtctagc
     1201 cgcacatgcc tgtccagtcg gggtacttcg attgctcagc ccgtgtagcc cttgtgcacg
     1261 cggccgcgcg gtgtcacgtc ctctgccgcc tcgtcgcccc atgcggccca gccgggttga
     1321 ggatggcggg cgaacagctc gagatacggt ccgggggagc acgcttcgat gaacggatac
     1381 tgttcatcgg gtttgcgtga atgctcccgt ttcctggttt cgatcatgtt gacctgactt
     1441 ctggcgtgcg gcagtgtccg catgcttccg cggacgccga acagaatcag ctcggtcaca
     1501 tttcggaagt agaagccgac gccgcggccg tcgggaccgc cgtcttgtcg ccgtttcgcc
     1561 cagatcacgt tcgacacgta gcgaaatccc cacgcgttca tgacccgaag cccttcgggc
     1621 agaagcgcat tcggtaccca caggtacagg tgcgaattcg gcgccgccac gtctgcgacc
     1681 gggatatcgc agatcgcatc aacgtcgagc gtcgaatagc ggtcaagcct gcgatgctcc
     1741 ggcgctacct ttccggtgcg gttcgtgaat cgccaaggcg ggtcggcgag caatgtcttc
     1801 caaccgccct ggctcgcggg gagggacggc ggttcggcta ctgcgcgcaa tggcgcggcg
     1861 cgaagacgtg tcccgtcatc gatctgctcc gacaacgtca cgctgccctc cctttgaaca
     1921 aagtagatct tcatcgaaca aggtagatct tcgatcggga gcgtaaatga gagcgccgac
     1981 gacatgcggg ccgcgtcggc tgcggtcaac gcgtttactg cagtgtcgtc acggcaagcg
     2041 gggcgtcgga gccggtgacg cggatgtcga ccgagcggat ctgtgcggta gggatcactg
     2101 tcgccgcggc gaggccggac gcctcgtcgc cggcgggacg ccacgatccc acggtggtgt
     2161 gcttgccgtc gacggtggtg acgaccaagt cgtagctacc gacgttcctc gaccagtcct
     2221 tggtgtattc gcagttccag tccaacctgg tgccccactg tttctccgtg acccccagct
     2281 ctgcggtgat tccgccgcgt ccggccgggg ccattgcctg cagcgccggc gcggtcgacg
     2341 gctgagggcg tccgaaaacc gacgccgtga cgatgacgcc gacggcgagg aacgcggctg
     2401 cggtagcggc caccaaagcc atgacgttgc gacgacttcg ccgctgccgt cgctgggccg
     2461 cgcgtgcaag cgacggcatc aggttcagtg gctgctggtc atccttgcca ttgggggtcg
     2521 catccagtgc caccgcctct tcagggctga gcacattgag aatacttggc atgccggcga
     2581 attcgctcag cgctgccgcc cgttccggat tggcggccaa atagccttcg tactcgattc
     2641 gctcctgcgg gcttagagcc ccgaggacgt aagcggcgtc ccagtgttcg atgtcattgt
     2701 cgtcggtcac cggacaaccc ccctttcctg caaggcattt cgtagcgctt tgagcgcgta
     2761 atgaagcctg gacttcacgg tgcctacagc gatctgctcg tgttcggcaa tgtcggcgac
     2821 tgtctgcccc agatagtagg cgcgcacaat cgcggtacgg tgctccttgc tgagcgacag
     2881 cagagcatcg gacagtatcc atttgtcgaa cgccggattg atggcgtctg cgagattccg
     2941 ctcgggcagg gtctcggtgc tgaattcgcg tgaataacga gagctgcggc ggtcgtcgat
     3001 cacgatgttc cttgcgacgg taaatagcca tgcccgcgcc gattccgagg gttgttctag
     3061 cacgtcgggc ttcttccaca gtctcaacag cgattcctgc acgacgtcct cggcgaactg
     3121 cgtgtccccg ttggtcag
//
LOCUS       DASWBF010000130         7609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_409318,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000130 DASWBF010000000
VERSION     DASWBF010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409318"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VF874_04460"
     CDS             complement(<1..831)
                     /locus_tag="VF874_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175367.1"
                     /translation="MTDDPVATAVQHDHELAALDLTEHPTVKAAYRTVAENWLARTKP
                     SDAMRARFDEAVAEVMFSAAVWSSNQDTLRPKVVCITRLGHPLAGRRIPGSRWGIDNP
                     DSVYRVIPISVDDRYEIHGRVGDNRMTENYFTLWDANMGTVDVLNGRTMQVDSDGSFT
                     ITVDSDPADGRPNHVQSTAAAHEFYIRDVLLNWDRDDPNHLAVERLGGPPSTPPRTLD
                     EQADATAAMMAHFANFTGKLSHGIYKMPPNHFNLAWSADKVGAMRNQVYVMGRFDLAP
                     D"
     gene            complement(824..2017)
                     /locus_tag="VF874_04465"
     CDS             complement(824..2017)
                     /locus_tag="VF874_04465"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9175368.1"
                     /translation="MGNSAFDMAGAMSAAQQKEGLDDWGPLPFEVPLAVLLESYAAAD
                     LNDIGTHKLRSGIVHSLRMRLRAHEWIKRHPEITDEPIVAPIVVVGMMRSGTTLAQRL
                     LAADPRFHCAYGWEVVEAAPKLDHRFAGIDPRIAISEAREAKSRELAPELFAIHPMYA
                     REPEEEIVFLSDVFLSHVPESGAHLPVYRSWIDDQDFTPAYDYLHRMLQFLQWQKRQR
                     GELAKAWVLKSPAHLGYLDTLRAQFPDLHLVHMHRDPREAVASGASLNATLHAMHSDT
                     VDLHRVGAEWLERMSWTNDRAMAARAGWADDAERVTDIEFADLVADPIGETSRVYDAI
                     GMELTADAQGAMRHWLAHRPREPQRPSYALEDYGLTDSQVDERFTAYRTRLRPGPEHE
                     GAVSD"
     gene            complement(2023..2466)
                     /locus_tag="VF874_04470"
     CDS             complement(2023..2466)
                     /locus_tag="VF874_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9175369.1"
                     /translation="MGASARQIENLIYTYAELLDAGDLDGVAQLFAHGRICGVENGPP
                     ETVFTGTAGVRQMYEMATRLYDDGTPKTKHNTSNVQIYVDEDAGTAHSRSYYCVTQAT
                     PELPLQVIVTGHYRDTFHRIDGVWWFDTRIMFIDQVGDMSHHLKF"
     gene            2525..3415
                     /locus_tag="VF874_04475"
     CDS             2525..3415
                     /locus_tag="VF874_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9175370.1"
                     /translation="MTSTWSPARLGDMTGKRVIVTGATNGVGLGTARALAKADAHVIV
                     AVRNTELGAQRAAEIGGSTTVSKLDLADLSSVRAFADRLDDDVDILVNNAGALAEKRS
                     DTVDGFEITIGTNLLGPFALTNLLFERVRSQIINVGSDAHKSATLRLDDMHLRTHKWT
                     ALGAYARSKLAVMLWGLELDRRLRAAQSPVVTHLTHPGFVASNLSHVSDRPLMAVAHR
                     AIKGAAGLVANDIDAGAAPTLYCITEPIPPGSYVGVDGVFGFRGAPVLIGRSVEACDY
                     EAAAEVFKFAEQETGTELPV"
     gene            complement(3489..4733)
                     /gene="lipE"
                     /locus_tag="VF874_04480"
     CDS             complement(3489..4733)
                     /gene="lipE"
                     /locus_tag="VF874_04480"
                     /EC_number="3.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase LipE"
                     /protein_id="HEX9175371.1"
                     /translation="MTEDSRIRVPDDLDSVTAIGEEDHSGIDAASVERIWQAARHWYA
                     AGMHPAIQLCLRHNGRVVLNRAVGHGWGNAPTDPPDADKVPVTTETPFCAYSAAKAIT
                     TTVVHMLVERGHFSLDDRVCDYLPNYTSHGKDRTTIRHVMTHSAGIPIHTGPRPDLRR
                     MDDSEYTREKLGELKPLYRPGLVHIYHGLTWGPLVREIVSAATGTNIRDILATEILDP
                     LGFRWTNYGVVPHDVPLVAPSHATGKPLPAPIARVFRLAVGGTLQKVIPFSNTPQYLT
                     SVLPSSNTVSTADELSRFAEILGRGGELDGVRVMRPETLRAAVKECRRLRPDVAVGLA
                     PLRWGTGYMLGSTRFGPFGRNAPAAFGHTGLTNIAVWADPERGLAAGLISSGKPGRHR
                     EADRYPALLDRITAEIPRVSAP"
     gene            complement(4730..5215)
                     /locus_tag="VF874_04485"
     CDS             complement(4730..5215)
                     /locus_tag="VF874_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEX9175372.1"
                     /translation="MADLHPSIAALAPLLGLWAGHGTGEYPTIQPFSYDEEVTFGHVG
                     KPFLTYNQRTKASDDGRPLHAEIGYVRVPSPGRVEWVLAHPTGIAEVQEGTLSISGTT
                     IEMELNATTIGLTESAKEVTALSRSIRVDGDELRYTVRMAAIGQWLQQHLAATLHRKH
                     T"
     gene            complement(5251..6069)
                     /locus_tag="VF874_04490"
     CDS             complement(5251..6069)
                     /locus_tag="VF874_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9175373.1"
                     /translation="MTVFASSDRGLAEPANVIELRRGGRALAGSYLYEGHKLVTGWHS
                     HDLHQIEYAIGGVVEVETETAHYLLPPQQAAWIPVGLQHQATMNPDVKTVAVMFDPQL
                     IPAGGDRARIVAVSPLIREMMVYALRWPIDRADSDAVSDGFFRTLAHLVSEALDHESP
                     LSLPTSDDPIVAAAMAYTKEHLSSVTADEVSRAVSVSERTLRRQFQAAVGISWRTYLQ
                     HARMLRAMALLAAPEQTVQQTCSAVGFDSLSSFTRAFTQFCGETPTSYRRRVRG"
     gene            6121..7383
                     /locus_tag="VF874_04495"
     CDS             6121..7383
                     /locus_tag="VF874_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9175374.1"
                     /translation="MNVDDLILVSIDDHVVEPPDMFLRHVPAKYRDEAPIVVTDDKGV
                     DQWIYQGKPQGVSGLNAVVSWPAEEWGRDPAGFAEMRPGVYDVHERVRDMNRNGILAS
                     MCFPTFTGFSARHLNQHREQVTVIMVQAYNDWHIDEWAGAYPDRFIPIALLPTWNPEA
                     MCDEIRRVAAKGCRAVTMPELPHLEGLPSYHDMDYWGPVFQTLSDENVVMCLHIGTGF
                     GAISMAKDAPIDNLIVLATQISAIAAQDLVWGPAMRSFPDLKFAFSEGGIGWIPFYLD
                     RCDRHYTNQKWLRRDFGGRLPSEVFREHALACYVTDKTSLKLRHEIGIDIIAWECDYP
                     HSDCFWPDAPEQVLAELEAAGADDTDINKITWQNACRFFSWDPFAKTPRERATVGALR
                     ASAADVDVSIRPRKEWARLYAERQLAKA"
BASE COUNT         1251 a         2491 c         2526 g         1341 t
ORIGIN      
        1 atccggtgcc aggtcgaacc ggcccatcac atacacctgg ttacgcatgg cacccacctt
       61 gtcggcagac cacgcgaggt tgaagtggtt gggcggcatc ttgtagatgc cgtggctgag
      121 cttgccggtg aagttcgcga aatgcgccat catcgccgcg gtggcgtcgg cttgttcgtc
      181 gagcgttcga ggtggcgtcg acggcggccc gccgagccgt tccacggcaa ggtgattcgg
      241 gtcgtcgcga tcccagttca gcagcacgtc ccggatgtag aactcgtggg cggcggcagt
      301 ggactggacg tggttaggcc gcccgtcagc cgggtcggag tcgacggtga ttgtgaacga
      361 tccgtcggaa tccacctgca tcgtgcggcc gttgagtacg tcgaccgtgc ccatgttggc
      421 gtcccacagc gtgaagtagt tctcggtcat ccggttgtcg cccacgcgcc cgtggatctc
      481 gtagcgatcg tccaccgaga tcgggatcac ccggtagacg ctgtcgggat tatcgatgcc
      541 ccaacgggat ccgggaatgc gccggccggc cagcggatgg cccaatcggg tgatacagac
      601 gaccttcggc cgcagcgtgt cctggttcga cgaccacacc gcggccgaga acatcacttc
      661 ggcgacggct tcgtcgaagc gggcccgcat cgcgtcggac ggctttgtgc gggccagcca
      721 gttctccgcg acggtgcgat atgcggcttt cacggtcggg tgctcggtca ggtccaacgc
      781 cgcgagctcg tgatcgtgtt gtaccgctgt cgcgaccgga tcgtcagtca ctcactgctc
      841 cttcatgttc gggtccaggc cgcaaccggg tgcggtaggc ggtgaagcgc tcgtcgacct
      901 gggagtctgt caacccgtag tcctcgagtg cataactggg acgctgcggc tcgcgagggc
      961 ggtgagccag ccagtgccgc atcgcgccct gcgcatccgc ggtcagctcc atcccgatcg
     1021 cgtcgtacac ccgcgaagtc tcaccgatcg ggtcggcaac caggtcggcg aactcaatgt
     1081 cggtgacccg ctcggcgtcg tcggcccagc ccgcccgggc ggccatcgcc ctgtcgttgg
     1141 tccaactcat ccgttccagc cactcggcgc cgacccgatg caggtcgacg gtgtcggagt
     1201 gcatcgcgtg cagggtcgcg ttcaggctcg cgcccgacgc gacggcctcg cgcggatcgc
     1261 ggtgcatgtg cacgaggtgc agatccggga actgcgcgcg caaggtgtcc aggtagccca
     1321 ggtgtgccgg cgatttgagc acccacgctt ttgcgagctc gccgcgctgg cgtttctgcc
     1381 actgcaggaa ctgcagcatc cggtgcagat agtcgtacgc gggggtgaag tcctggtcgt
     1441 cgatccagga ccgatacacc ggcagatgtg cgccggactc cggaacgtgc gacaggaaca
     1501 cgtcggagag gaacacgatt tcctcttcgg gttcccgcgc gtacatggga tgaatcgcga
     1561 acagctccgg tgcgagctcg cgggacttcg cttcgcgggc ttcgctgatc gcgatgcgcg
     1621 ggtcgatacc cgcgaaccgg tgatccagct tgggtgcggc ctcgacaacc tcccacccgt
     1681 acgcgcagtg gaaacgggga tcggcggcca gcagtcgttg cgccaacgtc gtgccgctgc
     1741 gcatcatccc gaccacgacg atcggcgcga cgatcggctc gtccgtgatc tcaggatgcc
     1801 gtttgatcca ctcgtgagcg cgtaaccgca tccgcaggct gtgcacgatc ccggaccgca
     1861 gtttgtgggt tccgatgtcg ttgaggtcag cggcggcgta gctctcgagc agcacggcca
     1921 acggcacctc gaacggcaac ggcccccagt cgtcgagtcc ttccttctgt tgtgcggccg
     1981 acatcgcgcc cgccatgtcg aaggccgagt tgcccatgcc ggtcagaact tcaggtggtg
     2041 gctcatgtcg ccgacctggt cgatgaacat gatccgggtg tcgaaccacc acacgccgtc
     2101 gattcggtgg aatgtgtcgc ggtagtgtcc ggtcacgatg acctgaagtg gcaattcggg
     2161 agtggcttgc gtgacgcagt agtaggagcg gctgtgcgcg gttccggcgt cctcgtcgac
     2221 gtaaatctgc acattgcttg tgttgtgctt ggttttgggt gtgccgtcgt cgtagagccg
     2281 cgtcgccatc tcgtacatct gcctgacacc ggctgtgccg gtgaacacgg tctccggggg
     2341 gccgttctcg acgccgcaga tccggccgtg ggcgaacagt tgcgccacgc cgtccaggtc
     2401 accggcgtcc agcagctcgg cgtaggtgta gatcaagttc tcgatctgac gtgcgctggc
     2461 gcccatttca ccgtaatcaa ccagccgccg accgactgtg tcaatggctc gctaccctga
     2521 caccgtgacg tcgacgtggt cgcccgcgcg gctcggcgac atgaccggca agcgcgtcat
     2581 cgtgaccgga gcgaccaacg gtgtcggcct cggcacagcc cgtgcgctgg cgaaggccga
     2641 cgcgcatgtg atcgtggcgg tccgcaacac cgagctgggc gcgcagcgtg ccgccgaaat
     2701 cggcgggtcg acaacggttt ccaagctcga tctcgcggac ctgtcctccg tgcgggcgtt
     2761 cgccgaccgg ctcgacgacg acgtcgacat tctggtcaac aacgcgggtg cgctcgccga
     2821 gaagcgctca gacaccgtcg acgggttcga gatcacgatc ggcacgaacc tgctcggacc
     2881 gttcgcactg accaatctgc tgttcgagcg ggtgcgctcg cagatcatca acgtcgggtc
     2941 ggacgcccac aagtcggcga cattgcggct cgatgacatg cacctgcgga cgcacaagtg
     3001 gaccgcgctg ggcgcctacg cacgctcgaa gctggcggtg atgttgtggg ggctggaact
     3061 ggatcgcagg ttgcgcgcgg cgcagtcacc ggttgtcacc cacctgactc atccggggtt
     3121 cgtggcgtcg aacctgtcgc acgtctcaga caggccgctg atggcggtcg cacaccgggc
     3181 gatcaagggg gcggccggct tggtcgcgaa cgacatcgac gccggggcag cgcccacgct
     3241 gtattgcatc accgagccca tcccgcccgg cagctatgtc ggcgtcgacg gcgtgttcgg
     3301 ctttcgcgga gcacccgttc tgatcggccg gtccgttgaa gcgtgcgatt acgaggcggc
     3361 cgcggaagtc ttcaagttcg ccgagcaaga gaccggcacc gagcttccgg tctagtccgc
     3421 gacactcaag cctctgcggc gacgcgccga tacgagtcgc aggtttgagt ttggaggggc
     3481 gagaccactc agggagcgct gacgcggggt atctcggccg tgatccggtc cagcagagcg
     3541 ggatagcggt cggcctcccg gtgcctgccg ggtttgccgc tgctgatgag ccccgccgca
     3601 agcccgcgct cggggtcggc ccagaccgcg atgttggtca atccggtgtg gccgaatgcg
     3661 gcgggtgcgt tgcgcccgaa cggcccgaag cgcgtcgacc ccaacatgta cccggtcccc
     3721 caccgcagcg gcgcaagccc gaccgcgaca tcgggtcgca accgccggca ttccttcact
     3781 gcggctcgca gagtttcggg ccgcatgacg cgcacgccgt cgagttcgcc tccacgaccc
     3841 aggatttcgg cgaaccggga cagttcgtcc gcggtcgaca cggtgttgga cgacggcagc
     3901 acactcgtca ggtactgcgg agtgttcgag aacggaatga ccttctgcaa cgtcccgccg
     3961 accgccagcc gaaagacccg cgcgatcggc gccggtaacg gcttgcctgt cgcgtgactc
     4021 ggcgcgacca gtggaacatc atgcggcaca acgccgtaat tggtccaccg gaagcccagc
     4081 gggtcgagaa tctccgtcgc caagatgtcg cggatgttcg tgccggtcgc ggccgagacg
     4141 atctcgcgga ccagcgggcc ccacgtcagc ccgtggtaga tgtgcaccag acccggccgg
     4201 tagagcggct tcagttcgcc gagcttctcg cgggtgtact cactgtcgtc catgcgtcgc
     4261 aagtccggcc gcgggccggt gtgaatcggg atgcctgcgc tgtgcgtcat cacatgccgg
     4321 atcgtcgtgc ggtccttgcc gtgactggtg tagttgggca ggtagtcgca gacgcgatcg
     4381 tcgagcgaga agtgtccgcg ttcgacgagc atgtggacga cggtcgtggt gatcgccttg
     4441 gccgcggagt acgcgcagaa cggtgtttcc gtcgttaccg gaaccttgtc ggcgtcgggc
     4501 ggatcggtgg gcgcgttacc ccagccgtgc ccgaccgcgc ggttgaggac gaccctcccg
     4561 ttgtgccgca gacacaactg aatcgcgggg tgcattcccg ccgcatacca gtgccgcgcg
     4621 gcctgccaga tccgttcgac ggacgcggcg tcgatcccgg agtggtcttc ttctccgatc
     4681 gcggtaacgg aatccaggtc gtcggggacg cggattctgc tgtcctcggt cacgtgtgct
     4741 tcctgtgcaa cgtcgcagcg aggtgctgct gcaaccattg gcctatggcg gccatgcgta
     4801 ccgtgtagcg cagttcgtca ccatccacgc gaatcgaacg gctcagtgcc gtgacctctt
     4861 tggccgactc ggtcagtccg atcgtggtcg cgttcagttc catctcgatc gtcgtcccgc
     4921 tgatcgacag cgtgccctcc tgaacttcgg cgatgccggt gggatgcgcg agaacccatt
     4981 cgacccggcc cggagacggc acccggacat agccgatttc ggcgtgcaac ggacgcccgt
     5041 catcgctggc cttggtccgc tggttgtagg tgaggaacgg cttgccgacg tgaccgaacg
     5101 tgacctcttc gtcgtaactg aacggctgaa tcgtcgggta ctcgccggtg ccgtggcctg
     5161 cccacaagcc cagcagcgga gcgagggcag cgatgctcgg atggaggtcg gccacgtccg
     5221 tcagggtacg gcgtggccga ggcgacgtag ctagccgcgc actcggcgcc ggtacgatgt
     5281 cggggtctcc ccacagaact gggtgaaggc ccgggtaaag gagctgaggc tgtcgaaccc
     5341 gacggccgaa cacgtctgct gcacggtctg ttccggtgcc gccagcagcg ccatcgcacg
     5401 gagcatccgg gcatgttgca ggtaggtccg ccacgaaatg ccaaccgccg cttggaattg
     5461 gcgtcgcagg gtccgttccg aaacggatac cgcccggctg acctcgtccg cggtcaccga
     5521 tgacagatgc tccttggtgt aggccatcgc cgccgcgacg atcgggtcgt cggacgtcgg
     5581 caagctcagc ggcgactcgt ggtccaacgc ctcggacacc agatgggcaa gggtccggaa
     5641 gaacccgtcg gacaccgcgt cgctgtccgc ccggtcgatc ggccaccgca acgcgtacac
     5701 catcatctcc cggatcagag gcgagacggc cacgatgcgc gcgcggtcgc cgcccgccgg
     5761 aatgagttgc ggatcgaaca tcaccgccac ggtcttgacg tccgggttca tggtcgcctg
     5821 gtgctgcaac ccgaccggga tccacgccgc ctgctgcggc ggcagcagat agtgcgccgt
     5881 ctccgtctcg acctcgacca cgccgccgat cgcgtattcg atctggtgca ggtcgtgtga
     5941 atgccatccg gtgaccagct tgtgcccctc gtacaggtag ctgccggcaa gcgcgcgacc
     6001 accgcgacgt agctcgatga cgttggccgg ttcggctaaa cctctgtccg aactcgcaaa
     6061 gacggtcacg cttaagcagg gtacaagtga gctatgaacg ccgcgcacgc gaggagcgat
     6121 atgaacgtcg acgatctgat cctggtaagc atcgacgacc acgtcgtcga gccgcccgac
     6181 atgttcctgc gccacgttcc cgcgaagtac cgggacgagg cgccgatcgt ggtgaccgac
     6241 gacaagggcg tcgaccagtg gatctaccaa ggcaagccgc agggcgtcag tggcctcaac
     6301 gcggtggtgt cgtggccggc cgaggagtgg gggcgcgacc cggccgggtt cgccgagatg
     6361 cggcccgggg tgtacgacgt gcacgaacgc gtccgggaca tgaaccgcaa cggcatcctg
     6421 gcgtcgatgt gctttccgac cttcaccggg ttctccgcgc ggcacctcaa ccagcatcgt
     6481 gaacaggtca ccgtgatcat ggtgcaggcg tacaacgact ggcacatcga cgagtgggcc
     6541 ggcgcctacc ccgaccggtt cattccgatc gcactgctgc cgacctggaa ccccgaggcg
     6601 atgtgcgacg agatccgccg ggtggccgcc aaaggctgcc gcgccgtgac catgcccgag
     6661 ctgccgcatc tcgaaggcct gcccagctat cacgacatgg actactgggg cccggtcttc
     6721 cagacgctct ccgacgaaaa cgtggtgatg tgcctgcaca tcggcaccgg cttcggtgcg
     6781 atctcgatgg ccaaggacgc gccgatcgac aacctcatcg tgctcgccac ccagatctcg
     6841 gccatcgcgg cgcaggacct ggtgtggggg ccggccatgc gcagcttccc cgatctgaag
     6901 ttcgcgttct cggagggcgg catcggctgg attccgttct atctcgaccg ctgcgaccgc
     6961 cactacacca accagaagtg gctgcggcgt gacttcggcg gccggctgcc cagcgaggtg
     7021 ttccgcgagc acgcgttggc ctgctacgtc accgacaaga cgtcgctcaa gctgcgccac
     7081 gaaatcggca tcgacatcat cgcctgggag tgcgactacc cgcactccga ctgcttctgg
     7141 cccgacgcgc ccgagcaggt gctggccgag ctcgaggccg ccggcgccga cgacaccgac
     7201 atcaacaaga tcacgtggca gaacgcgtgc cggtttttca gctgggaccc gttcgccaag
     7261 acgccacgtg agcgggccac cgtcggcgcg ctgcgggcct cggctgccga cgtcgacgtg
     7321 tcgatccggc ctcggaagga atgggcgcgc ctgtacgccg agaggcagct cgcgaaggcg
     7381 tagggcaact gcggattccc caccaacatg tggcggaatc gtggttaact tagtcgatgc
     7441 tcgcgaaggt tcggcgcatc ctgagtttcg agatgaccat cgccgaatgg atcggcaccg
     7501 cagccatgct ggcggttccc tatctgctcg tcggcgtgat ctggccgctc atccacaccg
     7561 accagctcag ccacagccac ggcgcagcca gggtcgtggc gttcctcgg
//
LOCUS       DASWBF010000131         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_994416,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000131 DASWBF010000000
VERSION     DASWBF010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994416"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VF874_04500"
     CDS             complement(<1..299)
                     /locus_tag="VF874_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HEX9175375.1"
                     /translation="MIRRLEALGLAVGLVAWSALPGRRHPVTQATLAAALVAITKAPV
                     GLRPPALWAGLRLGSAAAFVVAAAGAASTAVPRVRSAMADRTPPAAPATWLALRIP"
     gene            complement(311..1996)
                     /locus_tag="VF874_04505"
     CDS             complement(311..1996)
                     /locus_tag="VF874_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175376.1"
                     /translation="MHPFLLWAWGLVRLDFVGVAVGALFFCLSLTPSLLPRQWLFQGV
                     IGGINAAIGYGIGVLLGKAFKPRRRGWWPPNPRVLYGLKAVTVAVSASASVLMIIPAA
                     AWQRQVSNVMGIEGPSTLGYLRTLLIAGVVGGALVGVSRVLLDMIKALARYLIRRWGV
                     NDEVALFIGTAIVVVLIVTLVNGVLIRGFVGGSSAMFRPQNASIRPGIEQPTQPERSG
                     SPSSFAAWDTLGYQGRNFVATGPHADQLTELNGRPAKEPIRIYAGLATADTEDARVNV
                     ILSELQRTAAFGRELLVIVPTTGTGWVDPVAARAIESMYNGDTAIVALQYSYLPSWIS
                     FLADRQTSTDSGKHLIDAVHGRWAKQSPERRPKLALYGESLGSMAGQAAFGYLPDIEQ
                     MGFSSVLWVGPPNASSLWHALTVRRDPGTPEVQPRYDNGRTVRFSKGDDVATIAAPPW
                     QGTRVLFLQHASDPIVWWSPDLLFERPDWLREPPSRDRTASMRWYPIITFWQVGFDMT
                     NASGMPAGHGHNYGHSVLDGWVAVAPPDGWTPEDTERIRRSLEKLEAAGGPEF"
     gene            complement(2067..>2459)
                     /locus_tag="VF874_04510"
     CDS             complement(2067..>2459)
                     /locus_tag="VF874_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX9175377.1"
                     /translation="LQRERVLADVLTAASRDGLVSAAHDLSEGGLIQAVVESALAGET
                     GCRIVLPEDADPFVALFSESAGRVLVAVPRTEESRFRAMCEARGLPAVRIGVADHGSD
                     AVEVQGLFTVSLDELRATSERVLPELFG"
BASE COUNT          402 a          896 c          818 g          343 t
ORIGIN      
        1 ggtatccgca gcgcgagcca ggtcgccggt gcggccggcg gggtgcggtc ggccatcgcg
       61 ctgcgcaccc gggggaccgc ggtgctcgcg gcgccggccg ccgccacgac aaacgccgcg
      121 gccgagccca gccgcagccc cgcccacagc gcgggtggtc gcaaccccac gggcgccttc
      181 gtgatcgcca ccagcgccgc ggctagcgtc gcctgcgtga cgggatggcg gcggccgggc
      241 agtgcactcc aggccaccag accgaccgcc aggcccagcg cttcaagccg acgaatcatc
      301 ttgtctgaca ttagaattcg ggaccgccag cggcctccaa cttctcgagc gaccgccgga
      361 tgcgttcggt gtcctcgggt gtccacccgt caggcggtgc caccgccacc cacccgtcca
      421 gcacggagtg gccgtagttg tggccgtgac ccgcgggcat gcccgacgcg ttggtcatgt
      481 cgaagcccac ctgccagaac gtgatgatcg ggtaccaccg catcgacgcg gtgcggtcgc
      541 ggctcggcgg ttcacgcagc cagtcgggtc gttcgaacag cagatccggc gaccaccaca
      601 cgatcgggtc ggatgcatgc tgaagaaaca gcacccgggt gccctgccag ggcggcgccg
      661 cgatggtggc gacgtcgtcg cccttcgaaa atcgcacggt gcgcccgttg tcgtagcgcg
      721 gctggacctc cggcgtgccc gggtcgcggc gaacagtcag cgcgtgccac agcgaactcg
      781 cgttcggcgg tccgacccac agcaccgacg agaaccccat ctgctcgatg tcggggaggt
      841 agccgaacgc cgcctggccc gccatcgacc cgaggctttc gccgtacagc gccagcttcg
      901 gtcggcgttc cggcgattgt ttggcccacc gcccgtgcac cgcgtcgatc agatgcttac
      961 cggagtccgt cgacgtctgc ctgtccgcca ggaaggaaat ccagctcggc agatacgaat
     1021 actgcaaagc cacaattgcg gtgtcgccgt tgtacatcga ctcgatcgcg cgtgccgcga
     1081 ccggatcgac ccagccggtg cccgtggtcg gcacgatcac cagcagctcg cggccgaacg
     1141 cggcggtgcg ctgcagttcg gacaggatca cgttcacccg cgcgtcctcg gtgtcggccg
     1201 tcgccagccc cgcgtagatc cggatcggtt ccttggcggg gcgtccgttg agttcggtca
     1261 gctggtcggc gtgcggtccg gtggccacga agttgcggcc ttgatagccg agggtgtccc
     1321 acgccgcgaa agagcttggg ctgccggatc tttcgggctg cgtcggctgc tcgatccccg
     1381 gccggatgct ggcattctgc ggtcggaaca tcgcgctcga gccgccgacg aagccgcgga
     1441 tcagcacgcc gttgacgagc gtgacgatca gcacgacgac gatcgcagtg ccgatgaaca
     1501 gcgcgacctc gtcgttcacc ccccatcgtc tgatcaggta gcgagccagc gccttgatca
     1561 tgtcgagcag cacccgcgac acgccgacca gcgctccgcc gacgaccccc gcgatcagca
     1621 gtgtgcgcag gtaaccgaga gtcgacgggc cctcgatgcc catcacgttc gacacctgcc
     1681 gctgccaggc cgcggcgggg atgatcatca gcacgctggc cgacgcggat accgcaacag
     1741 tgaccgcctt caagccgtag agcaccctgg ggttcggcgg ccaccagccg cggcgccgcg
     1801 gcttgaacgc cttaccgagc agcacgccga tcccgtagcc gatcgcggca ttgatgccgc
     1861 cgatcacacc ctggaaaagc cattgccgcg gcagcagcga cggcgtcagc gacaggcaga
     1921 agaacagcgc accgaccgcc acgccgacaa agtccagccg gaccagaccc cacgcccaca
     1981 gcagaaatgg gtgcaggtcg cgttcctcgc ggtcggcggc cggcgcgccg gtcccgctcg
     2041 cggtgtcgac ggcgccgttc actcgcctac ccgaacaact ccggcagcac gcgctcggag
     2101 gtggcgcgca actcgtccag cgacaccgtg aacagccctt gcacctcgac cgcgtcgctg
     2161 ccgtgatccg ccacgccgat gcggacggcc ggcagtccgc gcgcctcgca catggcgcgg
     2221 aaccggctct cctcggtgcg ggggacggcg accagcaccc gcccggcgga ttcggagaag
     2281 agagcgacga agggatcggc gtcctctgga agtacgatgc ggcaaccggt ttcgccagcg
     2341 agcgccgatt cgaccaccgc ctggatgagc ccgccctcgg acaggtcgtg cgcggcggac
     2401 accagtccgt cccgcgaggc ggccgtgagc acgtcggcga gcacccgctc ccgctgcag
//
LOCUS       DASWBF010000132         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_140053,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000132 DASWBF010000000
VERSION     DASWBF010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140053"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..630)
                     /locus_tag="VF874_04515"
     CDS             complement(235..630)
                     /locus_tag="VF874_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175378.1"
                     /translation="MQRQSAGALTIRLLGRTDAEVNVETGLRAAEKLQSILDSRDRGV
                     DIAEMWVKLDRVINAVKSAVPEEMSGNIVEKLDQMEQYPESIIDEADAYRDSDDVFDP
                     TQLAEEDDEFRPPRITPTAEGWGPLPVFT"
     gene            complement(603..1691)
                     /locus_tag="VF874_04520"
     CDS             complement(603..1691)
                     /locus_tag="VF874_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX9175379.1"
                     /translation="MQRALVGAVLLALTFAGGYAVAVHKTVTLSVDGVPMTVSTMKSR
                     VIDVVRESGFAVGEHDNLYPGANQLVHQSDTIVLRRGRPLQVSIDGQPSKQVWTTALT
                     VDEALKQLSMSDAAPAAASRASRLPLAGMALPVVSAKHVHINDGGVASDQRLASPTVG
                     QLLAAAGAPLQQDDKVLPPAWMPVIEGMQIVVTRIRMHNVVARMALPAPLRRIQDPTL
                     NISRHVVEDPGTPGTQDVTFAVSTVNGVETGRRLVAHNVVTPARPSVQRVGAKPGTQV
                     PPVSNSATWDSLASCESSGNWAINTGNGFYGGVQFNQNTWERHGGLRYAPRADLATRE
                     EQIAIAEVTRARQGWGAWPVCSARVRAH"
     gene            complement(2067..2288)
                     /locus_tag="VF874_04525"
                     /pseudo
     CDS             complement(2067..2288)
                     /locus_tag="VF874_04525"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          412 a          932 c          823 g          430 t
ORIGIN      
        1 tcgaaggcgg gcgccggagt gcccagcgcg atcatggttg actcgacggc catgaaacga
       61 gcctaacttg catccatact ggcgccggcg gcgtttgccg gcgaaggcgc accgataatg
      121 taggtcggga ttcggtggcg gacatggccc ctcacaggtg tcgaagcgat ggaacagcga
      181 tggaagcagg gcgagacatc gctcgaacga tcccaggaag ccatcccccg accgctacgt
      241 gaacaccggg agcggtcccc atccctcggc ggtaggggtg atgcgcggag gtcgaaactc
      301 gtcgtcctcc tcggcgagtt gagtggggtc gaaaacatca tccgagtcgc ggtaggcgtc
      361 agcctcgtcg atgatcgact ctgggtattg ctccatctga tccagtttct cgacaatgtt
      421 gcccgacatc tcctcgggca ccgcagattt cactgcgttg atgacccggt cgagcttgac
      481 ccacatctcg gcaatgtcga cgccccggtc gcggctgtcc aggatggact ggagcttctc
      541 agccgcacgt agccccgtct caacatttac ctccgcgtcg gtccgcccga gcagccggat
      601 ggtcagtgcg cccgcactct ggcgctgcat accggccagg cgccccagcc ctgccgtgcc
      661 cgagtgacct cggcgattgc gatctgctct tctcgggtgg ccaggtcggc gcgcggcgcg
      721 taacgcagtc caccgtggcg ctcccaggtg ttctggttga actgcacgcc gccgtagaag
      781 ccgttgccgg tgttgatggc ccagttaccg ctggactcgc aggaggccag cgaatcccac
      841 gtggcgctgt tgctcaccgg cggcacctgg gtgcccggct tggcaccgac ccgctgcacc
      901 gacggccggg cgggtgttac gacattgtgg gccaccagcc gtcggccggt ttcgacgccg
      961 ttgacggtcg agacggcgaa ggtgacgtcc tgggtgccgg gagtgccggg gtcctcgacg
     1021 acgtggcggc tgatgttcaa tgtcgggtcc tggatgcgcc gcagcggcgc cggcagcgcc
     1081 atgcgcgcga cgacgttgtg catccggatg cgggtgacca cgatctgcat gccctcgatg
     1141 accggcatcc acgccggggg cagcaccttg tcgtcctgct ggagcggcgc accggccgca
     1201 gccagcagct ggcccacggt gggcgaggcc aggcgctggt cgctggccac accgccgtcg
     1261 ttgatgtgca cgtgcttcgc gctgaccacc ggcagcgcca tgccggccag cggcaagcgg
     1321 ctggcacgag acgccgcggc gggggcagca tcgctcatcg acagctgctt gagggcctca
     1381 tcgacggtca aggcggtcgt ccacacctgc ttgcttggtt ggccgtcgat cgacacctgc
     1441 agcggccggc cgcgtcgcag cacgatggtg tcggactggt ggaccagctg attggcgccc
     1501 ggatagagat tgtcgtgctc accgacggcg aagccgcttt cccgcaccac gtcgatcacc
     1561 cgcgacttca tcgtcgacac cgtcatcggt acgccgtcga cggacagcgt gacggttttg
     1621 tgcaccgcca cggcgtagcc gcccgcgaac gtcaacgcca acaagaccgc gcccaccagc
     1681 gcacgctgca ccttcgattt tgcggtgacc tttccgccgg tgcccgcccc ttcgccagcg
     1741 gcgctgtcat cttcgccgcc gcagtcgggt cgctgggcgg cccgctccgg ggcctgctgc
     1801 ccagcctcgg cctccagggc ggctttggcc ccgcggatct cggccagcgc agtctcgcgt
     1861 cgccgcggct gctcggccac ctcgccaccg cgactgtttt cgcccaacgc cgcgtcctcg
     1921 gacttgtcga tccgttcggc gtcggccaac agggctgaga cctcctcggc cagaacgttg
     1981 gcccgcaccc ttgtcccgtc cagcgcgact cggcctagcc ggaccatgcc cgcggcctgg
     2041 cacaacgcca acgcctgcac gaacagctgc cccagcgcag ccaggtgccg cgtgcggaat
     2101 cgggcgatcg ctcgatagtc cggggccgcc cccttggcca gccaccgaaa gcacacatca
     2161 tcgacgcatt tgcgttcgat cagccgcgac gagcgcaccc cgatgatgta gccgtacaac
     2221 aggacgcgga ccattagccg tggatcacag ggcggcgcgc cgcgcccctc ggtgcaggcg
     2281 gcccgcatgc ggacaagatc caaatgctcg tcgaccaaca ccgcaatgaa ccgggccaga
     2341 tgctcggccg gcaaccagtc atccagcgac ggcggcaaca acgatccctg atccggggcg
     2401 aatccaccga acgccttgtc caccgggcgg cttcggcctc gcgagatgcg tgtctgcccg
     2461 ctgtccaaga tctacctcga acaatccacc atcaccgagt tcatcagcca cccatcattc
     2521 cccgcgcccg atccccagcc gatcacccca cccggcgtgt tgcctaccca ggctccgcca
     2581 gccctgctgt tgctccc
//
LOCUS       DASWBF010000133         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_13596, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000133 DASWBF010000000
VERSION     DASWBF010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13596"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VF874_04530"
     CDS             <1..441
                     /locus_tag="VF874_04530"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX9175380.1"
                     /translation="QIHEARAHGADMLLLIVAALEQPALAAMLDRTESLGMTALVEVH
                     TEEEADRALQAGARVIGVNARDLKTLKVDRDCFARIAPGLPSNVIRIAESGVRGTADL
                     LAYAGAGADAVLVGEGLVTSGDPRSAVADLVTAGTHPSCPKSAR"
     gene            471..1733
                     /gene="trpB"
                     /locus_tag="VF874_04535"
     CDS             471..1733
                     /gene="trpB"
                     /locus_tag="VF874_04535"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX9175381.1"
                     /translation="MSTAKLPRSSAAVAEPTTHDPDSRGHFGVYGGRFVPEALMAVIE
                     EVTAAYEKARADQEFLDTLDRLQTHYSGRPSPLYEARRLTEHAGGATIFLKREDLNHT
                     GSHKINNVLGQALLAKQMGKTRVIAETGAGQHGVATATACALLGLECVIYMGAVDTAR
                     QALNVARMRLLGAEVVSVTSGSQTLKDAINEAFRDWVTNAERTYYCFGTAAGPHPFPM
                     MVRDFQRVIGLETRAQILAQAGRLPDAVTACVGGGSNAIGIFHPFIDEPGVRLVGFEA
                     AGEGVETGRHAATFTGGSPGAFQGSFSYLLQDEDGQTIESHSISAGLDYPGVGPEHAL
                     LKDTGRAEYRPITDTEAMDAFRVLCRTEGIIPAIESAHAVAGTLPLAAEIGRGGIIVV
                     NLSGRGDKDVATAAKWFGLLDDEDAARG"
     gene            1717..>2322
                     /gene="trpA"
                     /locus_tag="VF874_04540"
     CDS             1717..>2322
                     /gene="trpA"
                     /locus_tag="VF874_04540"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9175382.1"
                     /translation="MLRVVESARLAELFASCHHHDRSALIGYLPTGYPDVAMSIDAMT
                     ALVESGCDIVEVGIPYSDPGMDGPTIARATEAALRGGVRVRDSLRAVEAISDAGGRAV
                     VMTYWNPVLRYGVDAFARDLAAAGGLGMITPDLIPDEAAEWLAASDRHRLDRIFLVAP
                     SSTPQRLAMTVEASRGFVYAVSTMGVTGARDVVSRAAPELVR"
BASE COUNT          396 a          715 c          812 g          399 t
ORIGIN      
        1 cagattcacg aagcgcgggc ccacggcgcc gacatgttgc tgttgattgt cgcggcgctc
       61 gagcagccgg cgttggcggc gatgctcgac cgcaccgagt cgctcggcat gaccgcactg
      121 gtcgaggtgc acaccgaaga agaagccgac cgggcgctcc aggcgggtgc gcgggtgatc
      181 ggcgtcaacg cacgggatct caagacactc aaggtcgacc gggattgctt cgctcgtatc
      241 gcacccggcc tgccgagcaa cgtcattcgc atcgccgagt cgggcgttcg cggcaccgcg
      301 gacctgctcg cctatgccgg tgcgggtgct gacgcagtgc tcgtcggcga gggtttggtg
      361 accagcggtg acccgcgaag cgcggtagcg gatctcgtga cggcaggcac ccatccgtcg
      421 tgcccgaaat cggctcggtg agcggggatg agccgcttgc gcgaagaccg gtgagcaccg
      481 caaaactacc gcggtccagc gcggccgtcg ccgagccgac tacccacgac cccgactccc
      541 gtggccattt cggtgtctac ggtggacgat tcgtgcccga ggcgctgatg gccgtcatcg
      601 aagaagtcac cgcggcgtac gaaaaggccc gtgccgacca ggaattcctc gacacgctgg
      661 accgcctcca aacgcactac agcggcaggc cgtcaccgct gtacgaagcc cgccgcctca
      721 ccgagcacgc tggcggtgcg acgatcttct tgaagcgaga agacctgaac cacactggtt
      781 ctcacaagat caacaatgtg ctgggccagg cgctgctggc gaagcagatg ggcaagaccc
      841 gggtgatcgc cgaaaccggc gcaggccaac acggtgtcgc caccgcgacg gcgtgcgcac
      901 tgctcgggct ggagtgtgtg atctacatgg gtgccgtcga caccgcccgc caggcattga
      961 acgtcgccag gatgcgactt ctcggtgctg aggtcgtgtc ggtgacatca ggctcgcaga
     1021 cgttgaagga cgcgatcaac gaggcattcc gagattgggt gaccaacgcc gagcgcacgt
     1081 actactgctt cggcactgcg gccggtccgc atccgtttcc gatgatggtc cgcgactttc
     1141 agcgtgtgat cgggttggag acacgcgcac agatcctggc ccaggccggc cggttgcctg
     1201 acgcggtcac tgcctgcgtg ggcggcggtt cgaatgcgat cggcatcttc cacccgttca
     1261 tcgacgagcc gggtgtgcgg ctggtgggct tcgaagctgc gggtgaagga gtcgagaccg
     1321 gaaggcacgc agcaacattc accggaggct cgccgggcgc cttccaagga tcgttttcgt
     1381 acctgttgca ggacgaggac ggtcaaacca tcgaaagcca ttcgatctca gccggattgg
     1441 actatcccgg tgtcggacct gaacacgcgc tgttgaagga cacgggtcgt gccgagtacc
     1501 ggccgatcac cgataccgag gcgatggacg cgtttcgggt gctgtgccgc acggagggca
     1561 tcatcccggc gatcgagtcg gcgcacgccg ttgcgggaac gctgcctctg gcagccgaaa
     1621 tcggccgcgg cggaatcatc gtcgtgaacc tttccggacg cggggacaag gacgttgcca
     1681 cggcggccaa gtggttcggt ctgctcgatg acgaggatgc tgcgcgtggt tgaatccgcc
     1741 cggctggcag agttgttcgc ttcctgtcac caccatgatc gctcggcgct gatcggctat
     1801 ctaccgacag gctatccgga cgtagcgatg tcgatcgacg cgatgacggc gttggtggaa
     1861 tccggttgcg acatcgtcga agtgggaatt ccgtactcgg atccggggat ggacgggccg
     1921 acgatcgcga gggcgacgga ggcggcgctt cggggcggag tgcgggtacg cgattcgctg
     1981 cgcgcagtcg aggcgatcag tgacgcgggg ggccgcgcgg tggtgatgac gtattggaac
     2041 ccggtgctgc gatatggagt tgacgctttc gcacgcgacc tggccgctgc cggtgggctt
     2101 ggcatgatca cgcccgacct cattccggac gaggcggcgg aatggctggc cgcctcggac
     2161 cggcaccggc ttgatcgcat cttcctggtc gcgccgtcgt caacaccgca gcggcttgcg
     2221 atgacggtcg aggcgtcccg tggattcgtg tacgccgttt cgacgatggg cgtcaccggg
     2281 gcgcgcgacg tggtgtcccg agcggcgccg gagttggtgc gc
//
LOCUS       DASWBF010000134        11404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_599882,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000134 DASWBF010000000
VERSION     DASWBF010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599882"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            549..1709
                     /locus_tag="VF874_04545"
     CDS             549..1709
                     /locus_tag="VF874_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9175383.1"
                     /translation="MSVDVETATARVMRLPDLLRRRREPVIEDNDEAVVRQRRLTLIR
                     RIAVTTWVVVIVYRTATAGVAFNRELLLLYIATGLAAASIGRRKMLMVIRDWLPFAVV
                     LLVYDVSRGAAKLVGTPTLWEWQVNADRWLSFGTVPTVWLQERLKQAEPPWWEVSIST
                     VYMSFFILPYVVGGVLWLRDREDWKAFVRRFVALSFTALAIYIVLPAAPPWAAARCTG
                     PDIAHGPSYPNCMFSTPAGVPDGGLLGAMQATQPGAHEFVERISTRGFATLHLHVARD
                     LVNSGQVSVNMVAAIPSLHAALSVMIAAFLWRRVQRRWHPLLVAYVLMMAFTLVYSAE
                     HYVIDVLLGWALAAVVAWVIRQFESRRSAAPDEPLPHYDQPHDDAADCRAVP"
     gene            complement(1728..2810)
                     /locus_tag="VF874_04550"
     CDS             complement(1728..2810)
                     /locus_tag="VF874_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175384.1"
                     /translation="MAALPLAAIVDYRITADERARFKRCRRQWDFASPHRRDLEPVAP
                     VEPSVPSAVVEALAVYYYPGTWDWPHQLTQSLVHKALERSLNQKPAALATAAAVLDCY
                     DAWAVTVDDFAPIRIDHDVSGLLPDPDEPERGLSTTDGMAVVYSARVDLLAVDAADEY
                     WVVRHLIVDDWQDVDALLRDEEAVAACWAWEQDYIGMDIAGTIHNEVRIGGRLEMPAE
                     RASVRKQVAQHEPSGGGRSIPQHRRIYARASRSQSDKRIEQRTAGLVRRTRIRRGRDE
                     IASVGAQLAVEALEMIRNPAIYPTPAAHCRHCEFSAPCLAMMAAHDAELLLANGFRRH
                     PGESERKPRLGQTTWGFGRGAAPPQW"
     gene            complement(2792..3097)
                     /locus_tag="VF874_04555"
     CDS             complement(2792..3097)
                     /locus_tag="VF874_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175385.1"
                     /translation="MDREQLFASIVAARPDRDDVVYLERQGDQYQWRLVPAGNRIADG
                     SVSDEPDVWMSFSAAWPVDDPAQMRVFFDDLLAELESMADWADRCRWPIDEPWPHFH"
     gene            complement(3129..3536)
                     /locus_tag="VF874_04560"
     CDS             complement(3129..3536)
                     /locus_tag="VF874_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX9175386.1"
                     /translation="MAEFDAREQFTAGQVAFLATASGDGVPHVVPVVFAVAHDVVYTA
                     VDTKRKTTHRLRRLVNIEQNPQVSMLVDHYADEWDQLWWVRADGVAEIHDRGEAMATG
                     YDLLRRKYPQYQRISLTGPVISIAVSRWSSWHA"
     gene            complement(3554..4123)
                     /locus_tag="VF874_04565"
     CDS             complement(3554..4123)
                     /locus_tag="VF874_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9175387.1"
                     /translation="MGPKRADLVLSGGGVKGIGLVGAVVALMDAGYTAERVSGTSAGS
                     IVGAIVAAASQGQQMTGAEVKDLALSLDYRKFLDPGRWPSFGVTVLPNLPEGNDRVIP
                     ALSPLRVIGPPHLLEEVITTVLVGRDQAYLSQPWVSARAIRVDSTKVGFLDFDISDKE
                     VEALYGKGYAATEKFLETWDRRAYLRKFR"
     gene            complement(4162..4572)
                     /locus_tag="VF874_04570"
     CDS             complement(4162..4572)
                     /locus_tag="VF874_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEX9175388.1"
                     /translation="MAEEVWLIDKSALARINHVKSAATWANRIERGLVRLAAVTRMEV
                     AYSAQSGDDARQLFSMPPLAAIPVEYLTPAIEDRALEVQMLLADRGKHRAPSIPDLII
                     AATAELARLVVLHVDTDFELIASVTGQPVERLSL"
     gene            4921..5727
                     /locus_tag="VF874_04575"
     CDS             4921..5727
                     /locus_tag="VF874_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175389.1"
                     /translation="MKSIFITGAGSGMGREGAKLFHAKGWRVGAIDRNDEGLASLSSE
                     LGTDRIWTRAVDVTDKSALETALADFCAGNDGGGLDMMWNNAGIGEGGWFEDVPYEAA
                     MRVVDVNFKAVLTGAYVSLPYLKKSVGSLMFSTSSSSATYGMPRLAVYSATKHAVKGL
                     TEALSIEWARHGVRVADVLPGLIDTAILTNTPDRSDGRVLTHEELEARAPKKGMFRLM
                     PASSVADAAWRAYQHPTRLHWYVPESIRWIDRIKGLSPELTRRQIVKELP"
     gene            5768..6778
                     /locus_tag="VF874_04580"
     CDS             5768..6778
                     /locus_tag="VF874_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX9175390.1"
                     /translation="MAEVTSRSRVSSWMYALRTTNPPPDGPVDAVTRWIVVTRAAVLP
                     MTLFAGLVAALLAIGQPGLDWRWLVLAIVGIVLAHVANNLMNDLFDAQVGTDSENYPR
                     AMYAPHPVLSGLVSRRTLLLAILAVNLADLAILVVLTWARGWPVLAFALAGFVLSVAY
                     TAPPLRLKKRGLGEPDVFVVWGPLMVCGTYYSAVGTVGWDVLLASLPYGLLCTTVLMG
                     KHIDKIPYDTPLGIRTVPVILGESRARTATLAMIVGFYVLVAVCVAVGAMPWPALLVV
                     LALPRLVKVWPYFRKPPPSEPPPKFPIWPLWYAALAWLHVRQAGALLVLGLAIGAALR
                     VI"
     gene            7060..8859
                     /locus_tag="VF874_04585"
     CDS             7060..8859
                     /locus_tag="VF874_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9175391.1"
                     /translation="MVLVAIASLVATVAIPLMTKAVIDGPVRHQNQQGLWALGAAAMG
                     VGVAEAVLWFIRRWLVSRATMGVEADIRKDLYARLQILPMSFHGRWQSGQLLSRIMND
                     LSTIRRFLSFGLVFLILNGLQITVVTAILLAMYWPLGVVVLLSIVPIVATVLHFQQEY
                     TRLSRLAQDQSGHVATHVEEAALGLRVVKSFGREGYVYDRFDEQLTNLYDTQVERVSV
                     SAKFWTLLEIIPNLTLIIVLGFGAYAAGHGFVTMGTLVAFITMMLSLVWPIASLGFLL
                     SMTQESFTAANRISEIFDAPREITDGPHSQTPRGGRLELVDVGFRFPDAAPEDWALRH
                     VTVTVEPGETLALVGSTGSGKSVLAALLSRLYDVSEGGIRIDGQDIRELSLEALRQAV
                     ATAFEDPTLFSMSVAENLRLGRPDATDQQLAQAIDVAAAQFVYDLPFGLDTRIGEQGM
                     SLSGGQRQRLSLARAILAAPKILVLDDTLSALDVHTEAVVEEALRRVLHSVTGIVVAH
                     RASTVLLADKVALLQDGTIAHVGTHAELLASVPQYRYLLAADDELDDTTERSCGWQDD
                     ERRKRLSHTHDEREATDAACAECGFVTSEAERR"
     gene            8856..10709
                     /locus_tag="VF874_04590"
     CDS             8856..10709
                     /locus_tag="VF874_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9175392.1"
                     /translation="MTATDTQPVDLPEDQWRGRFDEARDDDLPIDEILPRRREARALL
                     GSLLRPYRVTVALLAAVVVVENVARLSVPLLIQRGIDHGIPPIVDGGSARTLMVVVAT
                     LCGVVLIQAISRVFFLRRSGRIGQKVLLELRRRVFRHFQRLDIAFHDRYTSGRVVSRS
                     TNDVEAIQDMLETGFDSLITAALTLVGTAVLLVALDWRLGLMCLAAFPILVALVWWFR
                     NESSKTYREVRESAALVIVQFVETMTGIKAVQAYRREPRNQEIFDDVADRYRAINEKT
                     FKLLAVFMPGVKLVGNLTTGVVLLYGGYRVLHGQMTIGTLAAFLLYLRMFFEPMQEIS
                     QFFNTFQSAASALEKLAGVLAEQPGIKDPAHPVTLTDVRGDIEFHDVQFSYVPNRFVL
                     PGLSLKVPAGQTVALVGTTGAGKTTIAKLIARFYDPTSGSVTLDGVDLRDLAQSELRR
                     HVVMVTQENFMFDGTVADNIRFGRPDATDDEIRSAATAVGADGFIDALPDGYDTDVAK
                     RGGRLSAGQRQLIAFARAFLADPAVLILDEATSSLDIPSERMVQRALQTVLADRTALV
                     IAHRLSTVQIADRVLVLEHGRLVEDGSPDELIARSDGRYAALHRAWLESLA"
     gene            11013..>11404
                     /locus_tag="VF874_04595"
     CDS             11013..>11404
                     /locus_tag="VF874_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175393.1"
                     /translation="MKSATHLGRAAVDVYRRTGADVPFGDPLPSHGCEMEGWFWRITD
                     AASGRVVVALCSVNRNPDGDWATVAVALHPGGFVRSAALDRAETDQSRFTVRAVTDPT
                     GALDARTERLRIDLDDVHLDLRFDDPFT"
BASE COUNT         1818 a         3664 c         3831 g         2091 t
ORIGIN      
        1 tgactggttg ccccagcagg tttcatttcg ttcatctgga ttgaatcaaa gtgatttaaa
       61 accgcgcccg aggctgcttc gggcattacc aggcgcggta ggtccgctga aaacgcagtg
      121 cttttcgcta cccagtaccg atgttgccgg ggtcagagac gctggggcaa ctggtacggc
      181 gggtgatctt tgaactcgcc gctgaagggc cgcccttagc gagtggcgcg tggtatggac
      241 tattgcaagt tagctgccga ccggtcacgc gcccagcgca cgcttgttgc acgcgaggcg
      301 cagtgggatt cacgcggtag catttcttgc cgccgagaat ttcggggcga tgaaggtcac
      361 ggtcgccagt cgacgctctt gccgccgtcg tagcggcggc cccctgaacg cgtgctgacc
      421 actgggttac caagtcgttt cctcccggcg acggacggga cgtccgcggg tacctagact
      481 tccagcagta gtggccgaga agctggtcgg ccgcggagtc gggatcggct ggctgaaggg
      541 gtcacgtctt gagcgttgac gtggagactg caaccgcgag ggtgatgcgg ctgcccgatc
      601 tgctgcgccg gcgtcgagag ccggtgatag aggacaacga cgaggccgtg gttcgccagc
      661 gccggctcac tttgatccgc cgaatcgctg tgacgacctg ggtggtcgtc atcgtgtacc
      721 ggacggcgac ggccggggtg gcgttcaacc gtgagcttct gctgctgtac atcgcgaccg
      781 gcttggccgc cgcgagcatc ggccgccgca agatgctgat ggtgatccgg gactggctgc
      841 ccttcgcggt ggtgctgctg gtctacgacg tgagccgcgg cgccgcgaaa ctggtcggca
      901 cgcccaccct gtgggagtgg caggtcaacg ccgaccgctg gctgtccttc ggcacggtgc
      961 cgaccgtgtg gctgcaagaa cgcctgaaac aggccgagcc gccctggtgg gaagtgtcca
     1021 tcagcaccgt ctacatgtcg ttcttcatcc tcccgtacgt ggtgggcggg gtgctgtggc
     1081 tgcgtgaccg cgaggactgg aaggcgttcg tgcggcgctt cgtcgcgttg tcgttcaccg
     1141 cgctggcgat ctacatcgtg ttgcccgcgg cgccgccgtg ggcggcggcc cgctgcacgg
     1201 ggcccgacat cgcgcacggg ccgtcctacc cgaactgcat gttcagcaca cccgccggtg
     1261 tgcccgacgg cgggttgctc ggtgccatgc aggcgaccca gcccggcgcc cacgagttcg
     1321 tcgaacggat ctccacccgc gggtttgcga ctctgcacct gcacgtggca cgggacctgg
     1381 tcaactccgg gcaagtcagc gtgaacatgg tcgcggcgat cccgtcgctg catgccgcgt
     1441 tgtcggtgat gatcgcggcg ttcctgtggc gccgcgtgca gcgcagatgg catccgcttc
     1501 tggtcgcgta cgtgctgatg atggctttca cgctggtgta ctcggctgag cattacgtca
     1561 tcgacgtcct gctcgggtgg gcgctcgcgg cggtggtggc atgggtgatc cgtcagttcg
     1621 agtcacgtcg ctccgcagcg ccggacgagc cactgccgca ctacgatcag cctcatgacg
     1681 acgccgctga ctgtcgagca gtcccgtgag atcccggtcg atgccgatca ccattgaggc
     1741 ggcgctgctc cccttccgaa gccccacgtc gtctgcccga ggcggggctt gcgttccgat
     1801 tcgccgggat ggcggcgaaa cccgttggct aacaagagtt ctgcatcatg cgccgccatc
     1861 atcgccaggc agggcgcact gaactcgcag tgccggcaat gtgcggcggg cgtcggatag
     1921 atggcggggt tacggatcat ctcgagtgcc tcgacggcca gttgcgcacc aaccgatgcg
     1981 atttcgtcgc ggccgcgccg gatccgggtg cgacgcacga gtccggccgt tcgttgctca
     2041 atccgcttgt cgctctggga ccttgacgcg cgcgcataga ttcgtctgtg ctgcgggatc
     2101 gaccggccgc cgccactggg ttcatgctga gcaacctgct tcctgacgct cgcgcgttcg
     2161 gcaggcattt ccagccgacc accgatgcga acctcgttgt ggatggtgcc ggcgatgtcc
     2221 atgcctatgt agtcctgctc ccacgcccag caggcggcga cggcttcctc gtcgcgcagt
     2281 aacgcgtcga cgtcctgcca gtcgtcgacg atcagatggc gaaccaccca gtactcgtcg
     2341 gcagcgtcga ccgccaggag gtcgacgcgc gcgctgtaga cgacggccat cccgtcggta
     2401 gtcgacaacc cgcgttcggg ctcgtcgggg tcgggcagca ggccgctcac gtcgtggtcg
     2461 attcggatcg gcgcgaagtc gtccaccgta actgcccacg cgtcatagca gtcgagaacc
     2521 gcggcggcgg tcgcaagcgc cgccggcttc tggttgagcg accgctcgag cgccttgtgc
     2581 accaacgact gtgtcagttg gtggggccag tcccaggtgc cggggtagta gtagacggcc
     2641 agggcttcga ccaccgcgga aggcacggac ggttcgacgg gcgcgacggg ttcgaggtct
     2701 ctccggtgcg gtgacgcgaa atcccactgc cttcggcagc gtttgaacct ggctcgctcg
     2761 tccgcggtga tccggtagtc cacgatggcg gctagtggaa gtgcggccac ggttcgtcga
     2821 tcggccagcg gcaacggtcc gcccagtcgg ccatcgactc caattcggcg agcaggtcgt
     2881 cgaagaacac ccgcatctgc gcggggtcgt cgaccggcca cgccgccgag aacgacatcc
     2941 acacgtccgg ctcatccgag acggatccgt cggcgatccg gttgccggcc ggtaccaagc
     3001 gccactggta ctggtcgccc tggcgctcta gatacacgac gtcatcgcga tcggggcgtg
     3061 ccgccacgat cgaggcgaag agctgttcgc ggtccatgtc acgcctcgtc gattcagcat
     3121 aaacctgatc aagcgtgcca cgaagaccac cggctcacag cgatgctgat cactggcccg
     3181 gtcagcgaaa tacgttgata ctgaggatat ttacgccgca gcaagtcgta gccggtggcc
     3241 atcgcctcgc ctcggtcgtg aatctcggca acaccgtcgg cgcgtaccca ccacagctga
     3301 tcccattcgt cggcgtagtg gtccaccagc atgctcacct gtgggttctg ctcgatgttc
     3361 accagccgcc ggagccggtg cgtcgtcttt ctcttggtgt cgaccgcggt gtacaccacg
     3421 tcatgggcga ccgcgaacac gacgggtacc acgtgcggca cgccatcgcc ggaggctgtg
     3481 gcgaggaagg ccacctgccc agcggtgaac tgttctcgtg cgtcgaattc ggccatcggg
     3541 tttctcctcg cggtcagcgg aacttgcgga ggtaggcgcg ccggtcccag gtctccagga
     3601 atttctcggt ggcggcgtag cccttcccgt acagggcttc tacctccttg tcggagatat
     3661 cgaagtcgag gaagcccacc ttcgtggagt cgacgcggat cgcgcgggcg ctcacccagg
     3721 gctggctcag atacgcctga tctctgccga cgagcaccgt ggtgatgact tcttcgagca
     3781 gatgcggcgg cccgatcacc cgcaaaggtg agagcgccgg gatcaccctg tcgttgcctt
     3841 cgggcagatt gggcagcacg gtgaccccga agcttggcca gcgacccggg tcgaggaact
     3901 tgcggtagtc gaggctgagc gcgagatcct tcacttcggc gccggtcatc tgttggccct
     3961 gtgaggcggc cgcgacgatc gcgccgacga tcgagccggc cgacgtgccc gacactcgct
     4021 cagcggtata gccggcatcc atcagcgcga cgacggcgcc caccaggccg atgcccttga
     4081 ccccgccgcc agacagcacg agatcagccc gtttgggccc catcggtctc gtcacgcttg
     4141 tctcctctcg aacgagcggc gtcagagcga cagtcgctcc accggttgac cggtcacgct
     4201 cgcgatgagt tcgaaatccg tatctacatg tagcacaaca agtctcgcca gttctgccgt
     4261 tgccgcgatg atcagatctg gaatcgatgg agcacgatgc tttcccctgt cggcaagtag
     4321 catctgcact tccagcgcac gatcttcgat cgccggagtc aagtattcga cgggtatagc
     4381 cgctaaaggt ggcatcgaaa acagctgccg tgcatcatcc cccgactgcg ccgagtaagc
     4441 cacctccatg cgggtcacgg cggcgagccg gacaaggccg cgctcgattc ggttcgccca
     4501 cgtggccgca ctcttgacgt gattgattcg agccagagct gacttgtcga tcagccaaac
     4561 ctcttcagcc aatcgagatc attcccatgc gcgcctcata ggggccggat cggcgagtcc
     4621 cgcgactctc tcagcgaagg cgtcaaggtc agccgcggtg accgatgtgc ggactgtctg
     4681 ggcatcttga gtcagacgac gacggatgta ttcgcttcgc gacagtcccg atcgtgacgc
     4741 aaggtcatcc agtgcagcca ggacgtcgtc cgaaatgcca cgaatcagaa tgtcgctcac
     4801 gctggacctc ctgatatcgg atgatatcac cgggtgtcgc cattgtgcgg cggaggcttc
     4861 tctgccgggt gcggtaggaa tagcgcttga acgccagcga atttggagga cggcatggcc
     4921 atgaaatcca tcttcatcac cggggcgggc agcgggatgg gccgcgaagg cgcgaaactg
     4981 tttcacgcga aaggctggcg cgtcggcgcg atcgaccgca acgacgaggg cctggcgtcg
     5041 ctgtcgagcg agttgggcac cgaccggatc tggacgcgcg ccgtcgacgt caccgacaag
     5101 tcggcgttgg agaccgcact ggccgacttc tgcgcaggca acgacggcgg cggcctcgac
     5161 atgatgtgga acaacgccgg tatcggggag ggcggctggt tcgaggacgt gccgtacgag
     5221 gcagcgatgc gcgtggtgga cgtcaacttc aaagcggtgc tcaccggcgc ctacgtgtcg
     5281 ctgccctatc tgaagaagtc ggtgggaagc ctgatgtttt ccacctcgtc gtcgtcggcc
     5341 acctacggga tgccgcgcct ggcggtctac tcggcgacca aacacgcggt caagggattg
     5401 accgaggcgc tgagcatcga gtgggcccgc cacggtgtcc gcgtggcgga cgtgcttccc
     5461 gggctgatcg acaccgcgat cctcaccaac accccggacc gttccgacgg aagggttctc
     5521 acgcacgagg agctcgaagc tcgggcgccc aagaagggca tgttccgcct gatgccggcg
     5581 tcgtcggtgg ccgacgcggc atggcgggcc taccagcatc cgacccgact gcactggtat
     5641 gtgcccgaaa gcatccggtg gatcgaccgg atcaagggtc tgagccccga gctgacacga
     5701 cggcagatcg tcaaggagct gccctgaatc tccggagacg gttgagcgac gggcctacgc
     5761 tgtgagcatg gccgaggtca cgtcgcggtc gcgtgtcagt tcgtggatgt acgcgctgcg
     5821 caccaccaac ccgccgccgg acggccccgt cgacgcggtg acgcgctgga tcgtggtcac
     5881 ccgcgcggcg gtgctgccga tgacactgtt cgcaggcctg gtcgccgcgc tgctggcgat
     5941 cggacaaccc ggcctggatt ggcgctggct ggtgctggcc atagttggga tcgtgctggc
     6001 gcacgtcgcc aacaacctga tgaacgacct gttcgacgcg caggtcggca ccgacagtga
     6061 gaactacccg cgggcgatgt acgcgccgca cccggtgctg tccgggctgg tgagccgacg
     6121 caccctgctg ttggcgatcc tggccgtcaa tctggccgac ctggcgatcc tcgtcgtgct
     6181 cacgtgggcg cgcgggtggc cggtgctggc gttcgcgctc gccgggttcg tgctcagcgt
     6241 cgcgtacaca gcgccgccgc tgcggctgaa gaagcgcggc ctcggcgagc cggacgtctt
     6301 cgtggtgtgg ggcccgctga tggtgtgcgg cacctactac tcggccgtcg gaaccgtggg
     6361 gtgggacgtg ctgctggcct cgctgccgta tggcttgctg tgcacgacgg tgttgatggg
     6421 caagcacatc gacaagatcc cgtacgacac gccgctgggc attcggacgg tgccggtgat
     6481 cctcggcgag tcgcgcgcac ggacggcgac gctcgcgatg atcgtcggct tctacgtgct
     6541 ggtcgccgtt tgcgttgcgg tcggtgcgat gccgtggccg gcgttgctgg tcgtgttggc
     6601 gctgccgcgg ctggtcaagg tgtggccgta tttccgcaag ccgccgccat ctgagccgcc
     6661 gccgaagttc cccatctggc cgctctggta tgccgcgctg gcgtggctgc acgtgcgtca
     6721 ggcgggcgcg ctgctggtgc tgggactggc gatcggcgcc gcacttcgcg tgatctgact
     6781 cagcagcagc gtcgcgagca gcgaaactga acccatggtc gtagatcgct ggaactcacg
     6841 accctggctt cactttcggc gctgtagacg ggccggcata acggagcctg attatcggtt
     6901 gcttgccgtg ggaagattga tcaagtcgtg gataaatctt tgaatcacgg tttgcggtcg
     6961 gcgcccgcaa tcgcgccgcc acccgcacgc agccgacctg gctcggatct gcgacggctg
     7021 gtgccgtact tgctgcctta tcgtgcgcgg tggatttcga tggtcctcgt ggccatcgca
     7081 agcctggtgg ccaccgtggc gataccgctg atgacgaagg cggtcatcga cggaccggta
     7141 cgccatcaga atcagcaggg cctgtgggcg cttggcgctg cggcgatggg tgtcggcgtc
     7201 gccgaggcgg tgctgtggtt catccgccgc tggctggtgt cgcgcgcaac gatgggagtc
     7261 gaagccgaca ttcgcaagga cctctacgcg cggctgcaga tcctgccgat gtcgttccac
     7321 ggccgatggc aatccgggca gctgctgtcg cggatcatga acgacctgag cactattcgg
     7381 cgcttcctgt ccttcgggct ggtgttcctg attctcaacg ggcttcagat caccgtggtg
     7441 accgccatcc tgctggcgat gtactggccg ctgggtgtgg tggtgctgct gtcgatcgtg
     7501 ccgatcgtgg ctaccgtgct gcactttcag caggagtaca cccggctatc acggctcgcg
     7561 caggaccagt ccggccacgt cgccacacac gtggaggagg ccgcactcgg gctgcgggtg
     7621 gtcaagtcat tcggtcgcga gggctacgtc tacgaccggt tcgacgagca gctcaccaac
     7681 ctctacgaca cccaggtcga aagggtgtcg gtgtcggcga aattctggac gctgctggag
     7741 atcatcccca acctcaccct gatcatcgtg ctcggcttcg gcgcgtacgc cgccggccac
     7801 gggttcgtca cgatgggtac cctggtcgcg ttcatcacga tgatgctgtc gctggtgtgg
     7861 cccatcgcat cgctaggctt cctgctgtcg atgacgcagg agtcgttcac cgccgcgaac
     7921 cggatctccg aaatattcga cgcgccaagg gaaatcaccg acggtccgca ttcgcaaaca
     7981 ccgcgcggcg ggcggctgga actggtcgac gtcggcttcc ggttcccaga cgcggcaccc
     8041 gaagactggg cactgcggca cgtcactgtc accgtcgagc cgggtgagac gctcgcgctg
     8101 gtcggctcga cggggtcggg caagtcggtg ctggccgcgc tgctgtcgcg actgtacgac
     8161 gtcagcgagg gcgggatccg catcgacgga caggacatcc gcgagttgtc gcttgaggca
     8221 ctgcggcagg cagtcgcgac cgcgttcgag gacccgacgc tgttctccat gtcggtggcc
     8281 gagaacctgc ggctgggccg accagatgcg acggaccagc agttggcaca ggccatcgac
     8341 gtcgccgccg cgcagttcgt ctacgacctg ccgttcggcc tggacactcg catcggcgag
     8401 cagggcatga gcctgtccgg cgggcaacgg cagcggctgt cgctggcgcg cgcgatcctc
     8461 gccgccccga agattctcgt gctcgacgac accctgtccg cgctggacgt gcacaccgag
     8521 gccgtcgtcg aagaggcact gcggcgagtg ctccactcgg tgaccggcat cgtcgtcgcg
     8581 caccgggcgt cgaccgtgct gctggccgac aaggtcgcgc tgctgcagga cggcaccatc
     8641 gcccacgtcg gcacgcacgc cgaactgctg gccagcgttc cgcagtaccg ctacctgctg
     8701 gcggccgacg acgaactcga cgacaccaca gagcgcagtt gcgggtggca ggacgacgag
     8761 agacgcaagc ggctgagcca tacgcatgac gagcgggagg ccaccgacgc ggcctgcgct
     8821 gagtgcggct tcgtcacctc ggaggccgag cgccgatgac cgccaccgac actcaaccgg
     8881 tcgacctccc agaagaccag tggcgcggcc gcttcgacga ggcccgcgac gacgacctgc
     8941 cgatcgacga aatcctgcct cgccgccgcg aagctcgcgc gctgctcggc tcgctgctgc
     9001 ggccctaccg ggtcacggtc gcgctccttg ccgccgttgt ggtggtggaa aatgttgcgc
     9061 gcctgtcggt tccgctcctc atccagcgcg gaatcgacca cggaatcccg ccgattgtcg
     9121 acggcggatc ggcgcgcacg ctgatggtgg tcgtcgccac cttgtgcggt gtggtgctga
     9181 tccaggccat cagtcgggtg ttcttcctgc ggcggtcggg gcggatcggt cagaaggtgc
     9241 tgttggagtt gcggcgccga gtgttccgcc atttccagcg gctggacatc gcgttccacg
     9301 accggtacac gtcagggcgg gtcgtcagtc ggtccaccaa cgacgtcgag gccatccagg
     9361 acatgctcga gacgggcttc gacagcttga tcaccgcagc gctgacgctc gtcggcaccg
     9421 cggtcctgct tgtcgcgctg gactggcgac tcgggctgat gtgcctggcg gcgttcccga
     9481 tcctcgtggc gttggtctgg tggttccgca acgagtcgtc gaagacctac cgcgaggtgc
     9541 gagagagcgc cgcgctggtg atcgtgcagt ttgtcgaaac catgaccggc ataaaggcgg
     9601 tgcaggccta ccgacgcgag ccgcgcaacc aagagatctt cgatgacgtc gcggaccgct
     9661 accgcgccat caacgagaag acgttcaagc tgctggcggt cttcatgccg ggagtgaagc
     9721 tggtcggcaa cctcacgacc ggcgtggtgc tgctctacgg cggctaccgg gtgctgcacg
     9781 gccagatgac gatcggcacg ctggcggcct tcctgttgta cctgcgaatg ttcttcgagc
     9841 cgatgcagga aatctcgcag ttcttcaaca cctttcagtc ggcggcgtcg gcgctggaga
     9901 agctggccgg cgtgctggcc gagcagcctg gcatcaagga cccggcgcac cccgtcacgc
     9961 tgaccgatgt tcgcggcgac atcgagttcc acgacgtgca gttttcctat gtgccgaatc
    10021 gtttcgtgtt gccaggtctt tcgctgaagg ttccggcggg ccagaccgtc gcgctggtcg
    10081 gtaccaccgg cgcgggtaag acgacgatcg ccaaactgat cgcccgcttc tacgacccga
    10141 cttcagggtc ggtgacgctc gacggcgtgg acttgcggga tttggcacaa agtgagttgc
    10201 gccgccacgt cgtgatggtc acccaggaga acttcatgtt cgacggcacc gtcgccgaca
    10261 acatccgctt cgggaggccc gacgctaccg acgacgagat ccgttccgct gcaacggctg
    10321 tgggcgccga cggattcatc gacgcgctac cggacggcta cgacaccgac gtcgccaagc
    10381 ggggtggccg gctgtcggcg ggccagcggc agctgatcgc gttcgcgcgg gcgttcctcg
    10441 cggatccggc ggtattgatc ctggacgagg cgacgtcgtc gttggacatt cccagcgagc
    10501 gcatggtgca gcgtgcgctg cagacggtgc tggccgaccg gaccgcgctc gtcatcgcac
    10561 accggctctc gaccgtgcag atcgccgacc gcgtgctggt cctggagcat ggccgcttgg
    10621 tcgaggacgg gtcgcccgac gagctcatcg cgcgcagcga cggtcgctat gcggcgctgc
    10681 accgtgcgtg gttggagtcg ttggcctaac gcgtcagctg tctttgtcgt cgtcatcgtc
    10741 gtcgtcgtcc tcggctcaaa ggaacaccga cgggcaaatt cgctcagacc tctgcgtcaa
    10801 tcccgggcga tgtaggcagc cccttctggg gcgacgggct gcaagaacgg ccgctacttt
    10861 ccagtccacg atcgcgtgcc atgcgatgct actggcatcc gtaccgcgct cgcgagccat
    10921 tccagcgtgt acgctgactg gctgtcacgc gaaatccgct aggcagcatg atgtttcgtc
    10981 gccaaggtga cggttggagg tctttcgaag cgatgaaatc agcgacgcat ctaggaaggg
    11041 cggcggtcga cgtctatcgg cgcaccgggg cggatgtgcc gttcggcgac ccgttgcctt
    11101 cgcacgggtg cgaaatggag ggctggttct ggcgcatcac ggacgccgcg tcgggacggg
    11161 tcgtcgtcgc gttgtgcagc gtgaaccgga atcctgatgg cgactgggcc accgtcgcgg
    11221 tcgcgctgca ccccggcgga ttcgtccggt ccgccgcgct ggaccgtgcc gagacagacc
    11281 agtcccggtt caccgttcgc gccgtgaccg acccgacggg cgcattggac gcccgcaccg
    11341 agcggctccg aatcgacctc gacgacgtgc acctcgacct gcggttcgac gatccgttca
    11401 catg
//
LOCUS       DASWBF010000135         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_536781,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000135 DASWBF010000000
VERSION     DASWBF010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536781"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VF874_04600"
     CDS             <1..815
                     /locus_tag="VF874_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175394.1"
                     /translation="TFFAEDADSHTLLYANADLSKASQANEVLAFADHWKKVSGRDPG
                     LLTFDSKVTTQKQLAELDDRGIRFLTLRARSPKLTAALHTLPVSAWKSLTVARAGGKT
                     RKVKVHEDPAARLTAYPGTVRQLAVAGLGHDEPTILITNDRDRAHKALIERYAQRMNI
                     EQRLAEAIRSFGLDALAGAVPLNVDLDVVLSVLAHTVCAALRRRLPGYATATPDTLQR
                     RFLHTGGIILNRANEIVVRLDRRAYSPVLRQADLPAVTVPWWGDRQLRYEFA"
     gene            complement(1099..>1654)
                     /locus_tag="VF874_04605"
     CDS             complement(1099..>1654)
                     /locus_tag="VF874_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX9175395.1"
                     /translation="YVGLWDWSQRTVIALVMQTEDNSLTLFPKRTRFGRIKLSSRQGH
                     GVPNPTWIPAANEAVRTLAREIDGTPWSSIGEILDIPMTAHFLGGCVIAESAERGVID
                     PYHRVFGHPGLHVFDGAAVSANIGVNPSLTITAQAERATAFWPNRGEADSRPPLGASY
                     QRISPVQPLKPVVPEHAPAALRLQ"
BASE COUNT          285 a          608 c          504 g          257 t
ORIGIN      
        1 tgacattctt cgccgaggac gccgacagcc acaccctgct ctacgccaac gccgacctgt
       61 ccaaggccag ccaggccaac gaggtgctcg ccttcgccga ccactggaag aaggtttctg
      121 gccgcgaccc ggggctgctg accttcgact ccaaggtcac cacccagaag cagctggccg
      181 aactggatga tcgcggcatc cggttcctca ccctgcgcgc ccgctctccg aagctcacag
      241 ccgccctgca cacgctgccg gtgtcggcgt ggaagtcgct gaccgtggcc cgggccggcg
      301 gcaagacccg caaagtgaaa gtccacgagg accccgccgc gcggttgacc gcctaccccg
      361 gcaccgtccg ccaactcgcc gtcgccggac tcggccacga cgagccgacc atcctgatca
      421 ccaacgaccg agaccgagcc cacaaggcgc tcatcgagcg ctacgcccag cggatgaaca
      481 tcgaacaacg cctcgccgag gccatccgct ccttcggcct ggatgccctc gccggggccg
      541 tcccgctcaa cgtcgacctc gacgtcgtac tctccgtgct cgcccacacc gtctgcgccg
      601 cgctacgccg ccggctgccc ggctacgcca ctgccacccc ggacaccctg caacgccggt
      661 tcctgcacac cggcgggatc atcctcaacc gcgcgaacga gatcgtcgtc cggctcgacc
      721 ggcgcgccta ctcgcccgtg ctccgccaag ccgacctgcc cgccgtcacc gtcccctggt
      781 ggggcgatcg ccaactccgc tacgagttcg cctgaacccg agacccgact ggggtctgat
      841 tcgctgcgtg aaaatcggcg ttagtgggtg ccccgtgccg cccatgatga gggcggtggc
      901 cgcgagcacc accgacgtcc acgtcgtcga gagagctaat agaaccgcgg ccatgaccgc
      961 gaggagagca attgccagcg acctcacagt gtggcgcatg tcagcctccg tgacagggcg
     1021 gctcgaaatg cagtttgtgg agcgcgcggc ccatctggcc acccctgccg cgcatcgttc
     1081 acgagtatcc gccgtggatt attgcagccg caacgccgcg ggcgcgtgct caggaacgac
     1141 gggcttaagc ggctggaccg ggctgatccg ttggtagctg gcgcccagcg gtggtcggct
     1201 gtcggcctcc ccccggttcg gccagaaggc ggtagcccgt tcggcctgtg ccgtgatggt
     1261 cagcgacggg ttcaccccga tgttggccga gaccgccgca ccgtcgaaga cgtgcaaacc
     1321 cgggtgaccg aacacccggt gatacgggtc gatgacaccg cgttccgcag attcggcaat
     1381 cacacatcca ccgaggaaat gcgcggtcat tgggatgtcg aggatctcgc cgatgctgct
     1441 ccatggcgtg ccgtcgatct cacgggcaag cgttcgcacc gcctcgttgg ccgccggtat
     1501 ccaggttgga ttgggcaccc cgtggccttg ccgcgacgac aacttgatgc gcccgaaacg
     1561 ggtgcgcttg gggaacaagg tcagcgagtt gtcctcggtt tgcatcacca gcgcaatgac
     1621 ggtgcgctgc gaccaatccc acagtccgac gtac
//
LOCUS       DASWBF010000136         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_935430,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000136 DASWBF010000000
VERSION     DASWBF010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935430"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VF874_04610"
     CDS             <1..938
                     /locus_tag="VF874_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9175396.1"
                     /translation="AATGAAAMAAEEAGKPAVTGPEQLTLGSMPRRLFAATPSRLAAF
                     DCPRRYRMTYLDRPTPSRGAPWAHNTLGAVVHLALHRWWMSPRAKRTAAMGAVLVARN
                     WQSDGFRDEAHSREWRARAGQWVERYLGGIDPDDEPAGVERTVATVTKRLALSGRVDR
                     IDDRGGELVIVDYKTGRHVPDADEARSAQALALYALAAARTLRRPCRRVELHHVPTGR
                     VVAAEHSAESLARQVTRAEATAEDIIVATDTWHGGAHPDDIFPPAPGPGCSWCDFRKH
                     CPEGRAAAPDREPWAGLPESTDTVAGARGSPMGVS"
     gene            1030..2139
                     /locus_tag="VF874_04615"
     CDS             1030..2139
                     /locus_tag="VF874_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011597469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9175397.1"
                     /translation="MPFVGAAALPVTGEQRAELMRMANSSVLPHRRVVQARALLLAAD
                     GVANEQIARRIGVDSDTVRRWRSRFADRGPAGVGVIAEGRGRKSSLVPGTVAEVVRLT
                     MSETPGDGSTHWTTRSMARRVGIGKDAVARIWADHGLKPWKVDTFKVSNDPYFEEKLV
                     DVVGVYLNPPARAVVFSYDEKTQCQALDRTQPSLPLTPGRAATMTHDYKRNGTIDLFA
                     AMNIATGQVLTDCQKAHAGTDVLRFFKQIDASVPRGLAIHVVLDNLSAHSGPEITKWL
                     AHKNRRRWHLHFTPTSSSWLNLIERWFKELTDKRLRRGRFTSVAELTEAITTWAAHWN
                     NDPKPFIWKATADEIIAKVQRGREALHQIKSTTDH"
BASE COUNT          368 a          722 c          786 g          353 t
ORIGIN      
        1 cggcggcgac tggggcggcg gcgatggcgg cggaggaggc tggtaagccc gcggtgaccg
       61 ggccggagca gctgacgctg ggatcgatgc cgcgtcggct gttcgcggcg acgccttccc
      121 ggctggcggc gtttgattgt ccgcggcgct accgcatgac ctatctcgat cgccccactc
      181 cgagccgggg cgcgccctgg gcgcacaaca ccctcggcgc ggtcgtacat ctcgcgcttc
      241 atcgctggtg gatgtcgcct cgagcgaagc ggacagcggc gatgggcgcc gtgctcgtcg
      301 cccggaattg gcaatcggat ggcttccgcg acgaggcgca ctctcgtgag tggcgcgctc
      361 gggcaggtca gtgggttgag cgttatctcg gcggcatcga tccggacgac gagccggccg
      421 gggtggagcg gacagttgca acggttacta agcggctcgc actgagtggg cgggtggacc
      481 gcatcgacga tcgcgggggc gaactcgtga tcgttgacta caagactggc cgccacgtgc
      541 ccgacgccga cgaagcgcgg tcagctcagg cgctggccct ctacgccctg gccgcggcgc
      601 gaacgttacg gcggccgtgt cgccgggtcg agctacacca tgtgcccacc ggccgggtcg
      661 tggcggcgga gcacagtgct gagtcgcttg cccggcaggt gacccgcgcc gaggcgacag
      721 ccgaggacat catcgtcgcg accgacacct ggcacggcgg cgcccacccg gacgacatct
      781 ttccgcccgc gcccggacca gggtgctcgt ggtgtgactt ccgcaagcac tgcccggaag
      841 ggcgcgcagc cgcacctgac cgcgagccgt gggcgggtct gcctgagtcc accgacaccg
      901 tggctggtgc ccgcggctca ccgatgggcg tcagctaggt ccgcgcgatc cgcacgttcc
      961 ccgcgccgcc ggagtcattc cccccgcggg ctagtgctgt gtctgcgaaa tagtgcggta
     1021 gtttgatgta tgccattcgt aggtgcggcg gcgttgccgg tgacaggtga gcagcgggcc
     1081 gagttgatgc ggatggcgaa ctcgtcggtg ttgccgcatc gcagggtggt gcaggccagg
     1141 gcgctgcttc tggccgccga cggtgtcgcc aacgaacaga tcgcgcggcg gatcggggtt
     1201 gactcggaca cggtgcgtcg gtggcggtcg aggttcgctg atcggggacc ggccggtgtc
     1261 ggggtgatcg cggagggtcg gggtcgtaag tcgtcgctgg tgccgggcac ggtagccgag
     1321 gtggtccggc tcacgatgag cgagacgccg ggtgacggct cgacgcactg gacgactcgg
     1381 tcgatggccc gcagggtcgg gattggcaag gacgcggtgg ctcggatctg ggccgaccac
     1441 ggcctcaagc cgtggaaggt cgacaccttc aaggtcagca atgatccgta cttcgaggag
     1501 aagctggtcg atgtggtcgg ggtgtatctc aatccgccgg cgcgggcggt ggtgttcagc
     1561 tacgacgaga aaacccagtg ccaggcgctg gatcgcaccc agcccagttt gccgctcaca
     1621 cccgggcggg ccgccacgat gacccacgac tacaagcgca acggcaccat cgacctgttc
     1681 gccgcgatga acatcgcaac cggccaggtg cttaccgact gccagaaagc tcacgcgggc
     1741 accgacgtgc tgcgcttctt caaacagatc gacgcgtcgg tgccgcgtgg actggcgatc
     1801 cacgtggtgc tggacaatct gtcggcgcac agcggccccg agatcaccaa gtggctcgcg
     1861 cacaaaaacc gccgccgctg gcacctacac ttcacgccca cgtcgagctc ctggctgaac
     1921 ctgatcgaac gctggttcaa agaactcacc gacaagcgac tgcgccgcgg ccggttcacc
     1981 agtgtcgccg agctcaccga ggccatcacc acctgggccg cgcactggaa caacgacccc
     2041 aagccattca tctggaaagc gaccgcagac gagatcatcg ccaaggtgca acgcggacgc
     2101 gaagcactcc accagatcaa atcaacgacg gaccactagc cggtctagtt gccacgcttg
     2161 gtccgtgagt ttcgttttga cgccgcgagc tgtccgagct ggtcagcgcg ttcacgagcc
     2221 gagttcgcg
//
LOCUS       DASWBF010000137         8561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_737109,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000137 DASWBF010000000
VERSION     DASWBF010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737109"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VF874_04620"
     CDS             <1..290
                     /locus_tag="VF874_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX9175398.1"
                     /translation="RFYFEFHETQGEGYLAHLHDPLAAAVALNPSLVQTRTATVDVEL
                     TGTLTRGMTIADWSGRWGRPPNARVGVGVDPDVFFDRFIERVGAFAQRVYS"
     gene            complement(281..1864)
                     /locus_tag="VF874_04625"
     CDS             complement(281..1864)
                     /locus_tag="VF874_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX9175399.1"
                     /translation="MSSRVLAVWCMDWPAVAAANAASLAATTPVAVTLANRVVACSAA
                     ARAAGVRRGLRRRESQARCPDLHVVAADPGRDARFFEPVTAAVDDLVPRVEVLRPGLL
                     VLSVRGAARYFGSESAAAERLVDTVSAAGAECQAGIADQLPTAVFAARAGRIVEPGAD
                     AAFLADLSIRELATEPSLSGDGRAELVDLLWRLGIRSIGQFAALSRADVASRFGADAV
                     VAHRFARGEPQRGPSGREPAAELNAEMACDPPIDRVDAAAFAGRSLASQLHQTLMSAG
                     VGCTRLAIHAVTANGEELNRVWRCAEPLTEDATADRVRWQLDGWLNSRFRRAGAQRPG
                     DRQGERPTAPVTMLRLQPVEVVSAEALQLPLWGGLGEEDRLRARRALVRVQGLLGPEA
                     VQVPVLSGGRGPAERITLVPLGDELVPRADPRQPWPGQLPEPSPAVLLDDPVELVDAQ
                     GNPVRITSRGMFSADPARLEGHGPLRWWAGPWPVDERWWGPERSRGRTARAQVLADGG
                     RAFLLCYRQRRWYLEGVYE"
     gene            complement(1869..2510)
                     /locus_tag="VF874_04630"
     CDS             complement(1869..2510)
                     /locus_tag="VF874_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175400.1"
                     /translation="MTAAISLDRCALDRAEQVEHLRRKMAAVSGKVGRGHAPPPPVEE
                     LIPDSKSLLPMPESLAEHLPATLPRGSVVVASGARSLTLQVVAAVTSAGGHAVVVGQP
                     DVGLLAAVEMGADLTRLALVPDPGTDPIEVAAVLLDGMDLVVLGLGGRSVPLSRARAV
                     TARARNKGCTLLVTDGDWQGARMRLEARVSGYEVGGRIRKVRLAITNGHSAHA"
     gene            complement(2697..4286)
                     /gene="guaA"
                     /locus_tag="VF874_04635"
     CDS             complement(2697..4286)
                     /gene="guaA"
                     /locus_tag="VF874_04635"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX9175401.1"
                     /translation="MTEPHAPVRPVLVVDFGAQYAQLIARRVREARVYSEVIPHTATV
                     EDVKARDPQAIVLSGGPASVYADGAPQLDPALFDLGVPVFGICYGFQAMAQALGGTVA
                     RTGTSEYGRTELKVVGGELHSGLPDTQPVWMSHGDAVTSPPQGFDVVASSAGAPVAAF
                     ENRARALAGVQYHPEVMHTPHGQQVLSRFLHEFAGIDSTWTPANIADTLVEQVRRQIG
                     DGRAICGLSGGVDSAVAAALVQRAIGDRLTCVFVDHGLLRSGERAQVERDFVAATGAR
                     LVTVDAADRFLEALNAVSDPEGKRKIIGREFIRTFEAAVRDTLGHWGHLPLAGDDSEV
                     KFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPRDLNFTLVEPLRLLFKDEVRAVGR
                     ELGLPEEIVARQPFPGPGLGIRIVGEVTAARLDTLRRADAITREELTAAGLDNQIWQC
                     PVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDAMTADWTRVPYETLERISTRITNEV
                     PEVNRVVLDITSKPPGTIEWE"
     gene            complement(4316..4621)
                     /locus_tag="VF874_04640"
     CDS             complement(4316..4621)
                     /locus_tag="VF874_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175402.1"
                     /translation="MGDLQGISYSGQPVPEAFRAVEPPVAVLLDLAALFPREPHRRGG
                     YHPAGLQMHKVVEGRLCCWGLCEQGNWWGLVTYPVSYGAHSRPVTHWVPAWMLRRKG"
     gene            4718..4978
                     /locus_tag="VF874_04645"
                     /pseudo
     CDS             4718..4978
                     /locus_tag="VF874_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
     gene            complement(4990..6726)
                     /locus_tag="VF874_04650"
     CDS             complement(4990..6726)
                     /locus_tag="VF874_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9175403.1"
                     /translation="MEPDFDVLIIGSGFGGSVTALRLTEKGYRVGVLEAGRRFADHEF
                     AKTSWNLRKFLWMPRLGMYGIQRIHLLRNVMILGGAGVGGGSLNYANTLYVPPEPFFA
                     DRQWSHITDWRAELMPHYEQAHRMLGVVMNPTFTDADRIMKEVADEMGVGDTFVPTPV
                     GVYFGQDGEKTPGKTVRDPYFGGAGPDRTGCIECGSCMTGCRYGAKNTLVKNYLGLAE
                     SAGAQVLPMRTVTGFEQLPDGGWRVDTVVTGRWLRRGKKSFTTRHLVMAAGTFGTQKL
                     LFKMRDKGRLPKISERLGVLTRTNSESIVGAGRFKVAPDLDLTHGVAITSSIHPSSDT
                     HIEPCRYGKGSNAMGILQTLMTDGSGPEGTDVPRWKQFLQQVAENPKGTIRLMNPRRW
                     SERTMIMLVMQHLDNSITTFTHRTKLGIRRVTSKQGHGEPNPTWIPVGNKVTRRVAEK
                     IHGVAGGTWGELFNIPLTAHYLGGAAIGDSPQTGVIDAYHRVYGYPTLLVVDGTAISA
                     NLGVNPSLSITAQAERAASLWPNRGETDLRPEQGEAYRRLDPIPPQHPLVPADAPGAL
                     RRLPIEPVSSAG"
     gene            complement(6854..7981)
                     /locus_tag="VF874_04655"
     CDS             complement(6854..7981)
                     /locus_tag="VF874_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX9175404.1"
                     /translation="MVEIGMGRTARRTFELDDITIVPSRRTRSSKDVSTAWQLDAYRF
                     EIPVVSHPTDALASPEFAIELGRLGGLGILNGEGLIGRHADVEAKIAQVIEAAEKEPE
                     PSAAIRLLQQLHAAPLDPDLLGAAIARIRDAGVITAVRVSPQSARSLTPVLMQAGIDL
                     LVIQGTIVSAERVARDGEPLNLKTFIAELDVPVVAGGVLDHRTALHLMRTGAAGVIVG
                     YGSTSGVTTSDEVLGISVPMATAIADAAAARREYLDETGGRYVHVLADGDIHTSGDLA
                     KAIACGADAVVLGTPLAASAEALGDGWFWPAAAAHPSLPRGALLQVAVGERPPLRQVL
                     GGPSDDPFGSLNLVGGLRRSMAKAGYCDLKEFQKVGLTVGS"
     gene            complement(8090..>8561)
                     /locus_tag="VF874_04660"
     CDS             complement(8090..>8561)
                     /locus_tag="VF874_04660"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9175405.1"
                     /translation="DGGLQYSGDIAKALAAGASAAMLGSLLAGTAEAPGELIFVNGKQ
                     FKSYRGMGSLGAMQGRSGGKSFSKDRYFQDDVLSEDKLVPEGIEGRVPFRGPLATVIH
                     QLTGGLRAAMGYTGSATIEELQHAQFVQITAAGLKESHPHDITMTVEAPNYNSR"
BASE COUNT         1317 a         3101 c         2837 g         1306 t
ORIGIN      
        1 tgcgtttcta tttcgagttt cacgagacgc agggtgaggg ctacctggcg cacctgcacg
       61 acccgcttgc ggcggcggtc gcgctcaacc cgtcactcgt gcaaacccga acggccacag
      121 tcgatgtcga gttgacgggt acgctgacgc gcgggatgac gatcgccgac tggtcggggc
      181 gctggggccg tccgccgaat gcgcgggtcg gcgtcggcgt cgatccggat gtgttcttcg
      241 accggttcat cgagcgggtc ggcgcgttcg cgcaacgggt ctactcgtag acgccctcga
      301 ggtaccagcg ccgctggcgg tagcacagca ggaacgcgcg tccgccgtcc gccagcacct
      361 gcgcgcgggc ggtgcggccc cgagagcgct cggggcccca ccagcgttcg tcgaccggcc
      421 acggtccggc ccaccagcgc agcggcccat gcccctccag tcgcgccgga tcggcggaga
      481 acatcccgcg actggttatc cgcaccggat tgccttgagc atcaacgagt tccacgggat
      541 cgtcgagaag aacggccggt gagggctccg gcagctgacc cggccacggc tgccgcgggt
      601 cggcccgggg aacgagctcg tcgcccagtg gcaccagtgt gatccgctcg gccggcccgc
      661 ggccaccgct cagcaccgga acctggaccg cctccggccc gagcaaccct tgcacccgta
      721 ccagcgcccg tcgcgcccgc agccggtctt cctcacccag cccgccccac agcggcagct
      781 gcagcgcctc ggccgagacc acctccacag gctgtagccg cagcatcgtc accggcgcgg
      841 tgggccgctc gccctgtcta tccccgggtc gctgcgctcc tgcccgccgg aagcggctgt
      901 tcagccagcc gtccagctgc cagcgcaccc ggtcggcggt ggcgtcctcg gtcagcggct
      961 ccgcgcagcg ccaaacccgg ttcagctctt ccccgttggc ggtgacggcg tggatggcca
     1021 gccgggtgca gccgacgccg gccgacatca gcgtctggtg cagctggctg gccagcgacc
     1081 ggcccgcgaa cgccgcggcg tccacccggt cgatcggtgg atcgcaggcc atctcggcgt
     1141 tgagttcggc tgcgggttcg cgccccgacg ggccgcgttg cggctcaccg cgggcgaacc
     1201 ggtgcgccac caccgcgtcg gcgccaaacc gggacgcgac gtcggcgcgt gacagcgccg
     1261 cgaactgacc aatgctgcga atccccaacc gccacagcag atctactagc tcagcacgcc
     1321 cgtccccgga caggcttggt tcggtggcca actcccggat cgacaggtcg gccaggaacg
     1381 cagcgtctgc accgggctcg acgatgcggc cggcgcgcgc ggcgaacacg gcggtgggca
     1441 actggtcggc gatgccggcc tggcattccg cgcccgccgc tgacaccgtg tcgaccagcc
     1501 gctcggccgc ggcggattca gagccaaagt agcgggccgc cccgcgcacc gacagcacca
     1561 gcaggccggg acgcaacacc tcgacgcgcg gcaccaagtc gtccaccgcg gccgtcaccg
     1621 gttcgaagaa tcgcgcatcg cgacccggat cggcggcgac cacatgcaga tccgggcagc
     1681 gggcctgcga ttcgcgacgg cgcagacctc gtcgcacccc ggcggcccgg gcggccgcgg
     1741 agcaggcgac cacgcggttg gccagcgtca ccgccaccgg cgtcgtcgcg gcgaggctcg
     1801 cggcattggc ggccgcgacc gcgggccagt ccatgcacca gacggccagc acccgtgagg
     1861 acatgacatc acgcgtgcgc cgaatggcca ttcgtaatcg ccagccgcac cttgcggatg
     1921 cgaccaccta cctcatagcc gctgaccctg gcctccagcc gcatcctggc gccctgccag
     1981 tcgccgtcgg tgaccagaag cgtgcagccc ttgttgcggg cccgcgcggt cactgcccgc
     2041 gcccgcgaca gcggcaccga ccggccgccg aggccgagca ccaccagatc catgccgtcc
     2101 agcagcacgg cggccacttc gatcgggtcg gtgccggggt caggcaccag cgccagccgg
     2161 gtcaggtccg cacccatctc cacggcggcc agcaggccga cgtcgggctg cccgacgacg
     2221 acggcatgac caccggccga cgtcaccgcc gccaccacct gcagcgtcag cgaccgggcg
     2281 cccgacgcca ccaccaccga accgcgcggc aacgtcgcag ggaggtgttc ggccagcgat
     2341 tcgggcatcg gcagcaaact tttggagtcg ggaatcagct cctcgaccgg gggcggcggc
     2401 gcgtggccgc ggccgacctt ccccgacacc gcggccatct tgcggcgcag atgttctacc
     2461 tgctcagccc ggtcaagcgc gcaccgatcc aggcttatcg ccgccgtcac agcagcacct
     2521 cctgaaccac ctgtttcacc atgtattcga aaatatgttc gatactgcga gtaaacaccc
     2581 acccgccgac accgtcaaga ccgattccag cgcgaaactc aagctgctgt gggtcccgcc
     2641 ggcgcgtcgc cgcagccgtt tacgtgtcgc gacgaacgcg ggaaccccga cacgctttac
     2701 tcccactcga tggtgccggg cggcttgctc gtgatgtcga gcaccacccg attgacctcc
     2761 ggcacctcgt tggtgatccg cgtcgaaatc cgctccagcg tttcgtacgg gacccgtgtc
     2821 cagtcggcgg tcatcgcgtc ttcactcgac accggccgca gcacgatcgg atgcccgtag
     2881 gtgcgcccgt cgccctgcac gcccaccgac cgcacgtcgg ccagcagcac caccgggcac
     2941 tgccagatct ggttgtccag tccggccgcg gtcagctcct cacgcgtgat cgcgtcggcc
     3001 cgccgcagcg tgtccagccg cgccgcggtg acctccccga cgatccggat gcccaagcct
     3061 ggacccggaa acggttggcg cgcaacgatt tcctcgggca ggcccagctc gcgaccgacc
     3121 gcccggacct catccttgaa cagcagccgc agcggctcga cgagtgtgaa gttcaggtcg
     3181 cgcggcaggc cgccgacgtt gtggtggctc ttgatgttcg cggttccgct gccgccgccg
     3241 gactccacga cgtctgggta gagcgtgccc tgcaccagaa acttcacctc agagtcatcc
     3301 cccgcaagcg ggaggtgccc ccagtgtccc aacgtgtctc gcaccgcggc ctcgaacgtg
     3361 cggatgaact cccggccgat gatcttgcgt ttgccctccg ggtcgctcac cgcgttcagc
     3421 gcctcgagga acctgtcggc ggcgtcgacg gtgaccagcc gggcaccggt ggccgccacg
     3481 aaatcccgct ccacctgggc gcgttcacct gaccgcagca ggccatggtc gacgaacaca
     3541 caggtcaacc ggtcgccgat cgcccgctgc accagcgcgg cggccaccgc cgagtcgaca
     3601 ccgccggaca gcccgcagat ggcccgtccg tcgccgatct gccggcgcac ctgctcgacc
     3661 aacgtgtcgg cgatgttcgc cggcgtccag gtggagtcga taccggcgaa ctcgtgcagg
     3721 aagcggctca gcacctgctg cccgtgcggg gtgtgcatga cctccgggtg atactgcacg
     3781 ccagccagcg cgcgcgcccg gttctcgaac gccgccaccg gcgcgccagc gctgctggcc
     3841 accacgtcga aaccctgcgg cggcgaggtg accgcgtcgc cgtggctcat ccacaccggc
     3901 tgggtgtctg gcagacccga atgcagctcc cccccaacga ctttcagttc agttctgccg
     3961 tactcgctgg tgccggtgcg ggccacggtc ccgcccaggg cctgggccat cgcctgaaac
     4021 ccgtagcaaa tgccgaacac cgggacgccg aggtcgaaca gcgcgggatc cagttgcggg
     4081 gcaccgtcgg cgtacacgct ggccggcccg cccgagagca cgatcgcctg cggatcacgc
     4141 gccttgacgt cctcgacggt cgcggtgtgc ggaatgacct ccgaatagac cctcgcctcg
     4201 cgcacgcgtc gagcgatcaa ctgcgcgtac tgcgccccga agtcgaccac caataccggt
     4261 cggacgggtg catgaggttc tgtcacgacg gtcagtctat gggcgccgtg cgcgctcagc
     4321 ccttgcggcg cagcatccat gccggcaccc aatgcgtcac cgggcggctg tgggcgccgt
     4381 agctgaccgg gtaggtgacc aggccccacc agttgccttg ctcgcacagg ccccagcagc
     4441 acagccggcc ctcgacgacc ttgtgcatct gcaaaccggc cggatggtac ccgccccggc
     4501 ggtggggctc gcgaggaaac agcgctgcca ggtcgagcag cacggcgaca ggcggctcga
     4561 ccgcgcggaa cgcttcgggc accggctggc cgctgtagct gatcccctgc agatcaccca
     4621 ccgatcgatc atacgttcga tgccctgagg tgcgaaagaa ctgctcgggt acctgtgacg
     4681 aaggagcctg atcgcatgac gaaagtgagg tcctggaccc cacctgcctg acggtatctc
     4741 gtgtttgcga tttcgcttgc agtggaggga ctttcgactg ttggtcgacg tgacgcacag
     4801 caacgccccc tataccctgc gggacggtcc ccacggcgag ctgacgatcc ggcgcgcggg
     4861 cgaaaagctt gagctccgca cggagtcgtc gtcgaccgtc gatcttcgca gctgtcagcc
     4921 gctgctccca ccgccgccgc agccgccggg ctgcgagccg aagcggcggc tcgcctgatc
     4981 ctgccgcgct cagccggccg agctgaccgg ctcgatcggt agacgccgca gggccccggg
     5041 cgcgtccgcc ggcaccaagg ggtgctgcgg cgggatcggg tcgagccgcc ggtaggcctc
     5101 gccctgctcg ggccgcagat ctgtctcgcc cctgttgggc cacagcgacg ccgcgcgttc
     5161 ggcctgggcg gtaatcgaaa gcgacgggtt cacaccgaga ttcgccgaga tcgcggtgcc
     5221 gtcgaccaca agcagcgtcg ggtatccgta cacccggtgg taggcgtcga tgacgcctgt
     5281 ctgcgggctg tcgccgatcg cggcgccgcc gaggtagtgc gcggtcagcg gaatgttgaa
     5341 cagctcgccc cacgttccac cggccacacc gtggatcttc tcggcgaccc tccgggtcac
     5401 cttgttgccg acggggatcc acgtcgggtt cggctcgccg tgaccctgct tgctggtgac
     5461 gcgccggatt cccagcttcg tgcggtgggt gaacgtggtg atcgagttgt cgaggtgctg
     5521 catcacgagc atgatcatcg tccgctcgct ccagcggcga gggttcatca gccggatggt
     5581 gcccttcgga ttctcggcaa cctgctgcaa gaactgcttc cagcgcggca catcggtgcc
     5641 ttcgggcccg gacccgtcgg tcatcagtgt ctgcaatatc cccatcgcgt tggagccctt
     5701 gccgtagcgg cacggctcga tgtgcgtgtc ggagctcggg tggatcgacg acgtgatcgc
     5761 cacgccgtgc gtcaggtcca ggtctggggc caccttgaac cgccccgcgc ccacgatcga
     5821 ctccgagttg gtccgggtca gcacgccgag gcgctcggag atcttgggaa gcctgccttt
     5881 gtcgcgcatc ttgaacagca gcttctgtgt gccgaacgtg ccggcggcca tcacgagatg
     5941 ccgagtggtg aacgacttct ttccgcggcg cagccaccgc ccggtaacga cggtgtcgac
     6001 gcgccatccg ccgtcgggca gctgctcgaa gccggtcacg gttcgcatcg gaagtacctg
     6061 tgcgccagcg ctttccgcga gaccgaggta gttcttcacc aacgtgttct tggcgccgta
     6121 gcggcaaccc gtcatgcacg agccgcactc gatgcagccg gtccggtccg gaccggcacc
     6181 gccgaagtag ggatcgcgca ccgtcttacc gggcgtcttc tccccgtcct gcccgaagta
     6241 cacgccgacc ggcgtgggca cgaacgtgtc gccgacgccc atctcgtcgg caacctcctt
     6301 catgatgcgg tcggcgtcgg tgaacgtcgg gttcatgacg acgccgagca tgcggtgtgc
     6361 ctgctcgtag tgcggcatca actcggcacg ccaatcggtg atatgtgacc actggcggtc
     6421 ggcgaagaac ggctccggcg gcacgtagag cgtgttggcg tagttcagcg agccgccgcc
     6481 gacgcccgcg ccgccgagga tcatcacgtt gcgcagcaga tgaatgcgct ggatgccgta
     6541 catgcccagc cgcggcatcc agaggaattt gcgcaggttc cacgacgtct tggcgaactc
     6601 gtggtcggcg aacctccggc cggcctccag cacgccgacg cggtagccct tctcggtcaa
     6661 gcgcaacgcg gtgacactgc cgccgaaacc cgaaccgatg atgaggacgt cgaagtcagg
     6721 ctccataccg caagtattgc cgtaccggcg ggtaactcac cagtgccgtc acgctcaacc
     6781 tggcgcgagg gtgcaccttt gcacccgaac cgcggcgtgt tggggtacag acccgcacgc
     6841 tcgcgccatg gggtcagctg ccgacggtca ggccgacctt ctggaattcc ttgaggtcgc
     6901 agtagccggc cttggccatg gaccggcgca agccgccgac gagattcagc gagccgaacg
     6961 ggtcgtcgga cgggccgccg agcacctgcc gcagcggcgg gcgctcgccg acggccacct
     7021 gcagcagcgc gccgcgtggc agcgacgggt gcgcggccgc ggccggccag aaccagccgt
     7081 cgcccagcgc ctctgccgac gcggccagcg gcgtgccgag caccactgcg tccgcaccgc
     7141 acgcaatggc cttggccagg tcgccggagg tgtggatgtc gccgtcggcc agcacgtgca
     7201 cgtaacggcc gccggtctcg tcgaggtact cgcggcgggc cgcggcggcg tcggcgatcg
     7261 cggtggccat cggcacgctg atgcccagca cctcgtcgct ggtggtgaca ccgctggtgg
     7321 agccgtagcc gacgatgacg cccgccgcgc cggtgcgcat cagatgcagc gcggtgcggt
     7381 ggtcgagcac accgccggcc actaccggca cgtcgagttc ggcgatgaac gtcttgaggt
     7441 tgagcggttc gccgtcacgg gcgacgcgct cggcggacac gatcgtgccc tggatcacca
     7501 gcaggtcgat gccggcctgc atcagcacgg gcgtcagcga gcgcgcgctc tgcgggctga
     7561 cccgcaccgc ggtgatcacg cccgcgtcgc gaatgcgggc gattgccgcg ccgagcaggt
     7621 ccggatcgag cggcgcggcg tgcagttgct gcagcagccg gatcgccgcc gacggttccg
     7681 gctccttctc ggcggcctcg atgacttggg cgatcttcgc ctcgacgtcg gcgtgccgac
     7741 caatcagccc ttcgccgttg aggattccga gcccgccgag ccggcccagc tcgatcgcga
     7801 actccggcga cgccagcgcg tcggtggggt gcgacacgac cgggatttcg aatcggtacg
     7861 cgtcgagctg ccaggcggtc gacacgtctt tcgacgaacg ggtgcgccgc gacggcacga
     7921 tggtgatgtc gtcgagctca aaggtgcggc gggcggtgcg gcccatgccg atctcgacca
     7981 tgtcgcgcat gtctagcgcc cctcccctgc gaaaccgggg cttcgcacag aaagtccgag
     8041 aagcagggtg cgaaactccg gtttcggcaa catcagagag gatcctgcct cagcggctgt
     8101 tgtagttcgg cgcttcgacc gtcatcgtga tgtcgtgtgg gtggctctcc ttgagccccg
     8161 ccgcggtgat ctggacgaac tgcgcgtgct gcagctcctc gatggtggcc gagccggtgt
     8221 agcccatcgc cgcccgcagg cctccggtga gctggtggat gaccgtggcc agcgggccac
     8281 ggaacggcac ccggccctcg atcccttcgg gtaccagctt gtcctcggat agcacgtcgt
     8341 cctggaagta gcggtctttg gagaacgact tgcccccgga ccgcccctgc atcgcaccca
     8401 gtgatcccat gccgcggtag ctcttgaact gtttgccgtt gacgaagatc aactcgccgg
     8461 gcgcttccgc ggtgccggcc agcagtgagc cgagcatcgc ggcggacgca cccgcggcca
     8521 gcgccttggc gatgtcgccc gagtactgca gtccgccgtc g
//
LOCUS       DASWBF010000138         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_211091,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000138 DASWBF010000000
VERSION     DASWBF010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211091"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VF874_04665"
     CDS             <1..538
                     /locus_tag="VF874_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX9175406.1"
                     /translation="ELGSHQGLPVTIVATVDLADLQAKAGVATTGGGSWLPMTDVIRM
                     AAQADNFLLIFDDAKPLQLYKGRSTRLATPAHRLVLYALERGCSHPGCDVPACWCQAH
                     HAEKDWAAGGQTNIDELTLACGPHNRKVTKDGWKTRKRQDGTTEWIPPPHLDHGQRRT
                     NNYFRPERMLDEDDDQPE"
     gene            complement(555..989)
                     /locus_tag="VF874_04670"
     CDS             complement(555..989)
                     /locus_tag="VF874_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9175407.1"
                     /translation="MAATEESKALMWRITDEIWNNGRLDLIDELIAEDLVDHVELAGL
                     EGSGRARYRASVELTRAAFPDYRNPLDFVVAEGALAVSYGRSTGTHRGEYFGIPATGR
                     TIDVPTFGILRFENGQAVERWGMSDSLAIMRQLGLMGSSTSH"
     gene            1159..1362
                     /locus_tag="VF874_04675"
     CDS             1159..1362
                     /locus_tag="VF874_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175408.1"
                     /translation="MAGNEIDRVRARSALDVVKQHPVMVLFAVSPVIAAAVLVGWLTN
                     PVWAVLLLIVATVAGTWAVVRRR"
     gene            1419..1985
                     /locus_tag="VF874_04680"
     CDS             1419..1985
                     /locus_tag="VF874_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175409.1"
                     /translation="MRPHHVHGRRFGRPGGWQQADQPDAADAADWFAGRLPENWFTED
                     PEVTVDREEIAVIGRLPEPEGAEGEGADARGAGRAARFREETRPDRMRIADEAQARYG
                     RNVSWGVRVGGERIMFTNMAVPVMTRLRQPDRQVLDTLVDAGVARSRSDALAWCVRLV
                     GEHADEWLDELRQAMSTVDDLRAKGPEL"
     gene            complement(1982..>2889)
                     /locus_tag="VF874_04685"
     CDS             complement(1982..>2889)
                     /locus_tag="VF874_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX9175410.1"
                     /translation="RESHRHAHWLLPAVHWLERDDLMAFTSNLHDEPYVQYGRRAVEP
                     PPGAREEWRIFVDLTIAMRKPLFGVKGVNGFVEATRTLSRLTRRPALEFNPDWLNRLL
                     VATGRKVNGRKVRWRDIMAHPHGLVLGPREFGHLRDALRTEDKKIHAAPPEIVARTRE
                     LLAAPRPQAPDSYPFQLANRRNRHSMNSWLNELPGLHPSGKRNEVAINPKDAAELGIT
                     DGDQVRVFSSVGSIEVAATVSDRPRAGVVIVDHGWGSRVFDPRGGGAPESYGANRNLL
                     IDGAAVDPLSQTPPLNSSYVGVERA"
BASE COUNT          512 a          977 c          919 g          481 t
ORIGIN      
        1 tgagctgggc agccatcagg ggttgccggt gacgatcgtg gccaccgttg acctggcgga
       61 tctgcaggcc aaggccgggg tggccaccac cggcgggggc agctggctgc cgatgaccga
      121 tgtgatccgg atggccgccc aggccgacaa ctttctgctg atcttcgatg acgccaaacc
      181 cctgcagctc tacaagggcc gcagcacccg gctggccacc cccgcccacc ggctggtgct
      241 ctatgccctc gagcgtggct gcagccatcc gggctgtgac gtgccggcct gctggtgcca
      301 agcccaccac gccgaaaaag actgggccgc cggcgggcag accaacatcg acgaactcac
      361 cctggcctgc ggaccccaca accgcaaggt cacaaaagac ggctggaaaa cgaggaaacg
      421 ccaagacggc accaccgaat ggattccccc accgcatctg gaccacgggc aacgccgcac
      481 gaacaactac tttcgtccag aacggatgct cgacgaagac gacgatcaac cggagtagcc
      541 gggtggtctt tgaatcagtg gctggtgcta gagcccatca atcccagctg gcgcataatc
      601 gcgaggctgt ctgacatccc ccatcgttcg acggcctgac cgttctcgaa gcgcagaatc
      661 ccaaacgtcg gcacgtcgat cgtgcgaccg gtagcgggta tcccaaagta ctcgccccgg
      721 tgggttccgg tactccgtcc gtaggaaaca gcaagcgcgc cttctgcgac cacgaagtcc
      781 aggggattgc ggtaatccgg gaaagctgca cgcgtcagct cgacgcttgc tcgataacgg
      841 gctcgaccgc ttccctcaag accggcaagt tcgacgtgat cgacgaggtc ctcggcgatg
      901 agctcgtcga tcagatccag ccgaccgttg ttccagattt cgtcggtgat gcgccacatc
      961 agcgccttcg actcttcggt tgccgccatc gccaatcctt tccggaaatg gtgaccttaa
     1021 cttggacatc cgatagtcgt tgatggaacc gccgttccct tcacaacggt gcaccgagcg
     1081 tcagcgcgaa tgtgtgcaca attttggcgg tccctccgac cggcgcacga gcgccgctcc
     1141 cgctggcatc cttaatgcat ggcaggcaat gaaatcgacc gcgtcagggc acgtagcgca
     1201 ctggacgtcg tcaaacaaca tccggtgatg gtgctgttcg ccgtctcacc cgtgatagca
     1261 gccgcagtcc tcgtcggctg gctcacgaat ccggtttggg cggtactgct gctgatcgtc
     1321 gcgacagtgg ccggcacctg ggcagtggtt cgcaggcgtt agtttcgccc tcagcgaacg
     1381 ctctcgctgc aacgatgtaa gagtgtaagc atgtaatcat gcgaccacac catgtacacg
     1441 ggcggcggtt cggccgcccc ggcggctggc agcaagcgga ccagcccgac gccgccgacg
     1501 cagccgactg gttcgccggt cggctgcccg aaaactggtt caccgaggac cccgaggtca
     1561 cggtcgaccg ggaagagatc gccgtcatcg gtcggctccc agaacccgaa ggcgccgagg
     1621 gtgagggcgc tgacgcccgc ggcgccggcc gcgcggcacg cttccgcgag gaaacccggc
     1681 ccgaccggat gcggatcgcc gacgaggcgc aggcccgcta cggccgaaac gtcagctggg
     1741 gcgtgcgggt cggcggcgaa cgaatcatgt tcaccaacat ggccgttccg gtgatgaccc
     1801 ggctgcgtca acccgatcgc caagtgctcg acacgctcgt cgacgccggc gtcgcgcgct
     1861 cccgatcgga cgcgctggcg tggtgcgtcc ggctggtcgg cgagcacgcc gacgagtggc
     1921 tggacgaact ccgccaggcg atgtccacgg tcgacgatct gcgcgcgaaa ggcccggagc
     1981 tctaagcccg ttcgactccg acgtaggagg agttcagcgg cggcgtctgc gacagcgggt
     2041 ccaccgctgc accatcgatg agtaggttgc ggttggcgcc gtacgactcc ggagcgccgc
     2101 cgccgcgcgg gtcgaaaacc cgtgatcccc agccgtggtc gacgatcacg actccggcgc
     2161 gtggcctgtc gctcaccgtc gccgccacct cgattgaacc gaccgacgag aacacccgca
     2221 cctggtcgcc gtcagtgatg cccagctccg ccgcgtcttt cgggttgatc gccacttcgt
     2281 tgcgcttccc cgacgggtgc agaccgggaa gttcgttgag ccacgaattc atcgaatgcc
     2341 ggttgcgccg gttcgccagc tggaacgggt agctgtccgg tgcttgcggg cgcggcgcgg
     2401 cgagcagctc tcgggtccga gcgacgatct cgggcggcgc cgcgtggatc ttcttgtcct
     2461 cggtcctcag tgcgtcccgc agatgtccga actcccgcgg cccgagcacc agcccgtgcg
     2521 gatgtgccat gatgtcgcgc catctgacct tgcgcccgtt gacttttcgg ccggtagcca
     2581 ccagcagccg gttgagccag tccggattga actcaagggc cggcctgcgc gtgaggcgcg
     2641 aaagcgtgcg ggtggcttcg acgaaaccgt tgacaccctt gacgccgaac agcggcttgc
     2701 gcatcgcgat cgtcagatcg acgaatatcc gccactcctc gcgcgcgccg ggcggcggct
     2761 cgacggccct gcgcccgtac tgcacgtacg gctcgtcgtg caggttgctg gtgaaagcca
     2821 tcaagtcgtc acgctccagc cagtgcaccg cgggcaacag ccaatgcgcg tgccgatggc
     2881 tttcgcgct
//
LOCUS       DASWBF010000139         9393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_600171,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000139 DASWBF010000000
VERSION     DASWBF010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600171"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF874_04690"
     CDS             complement(<1..189)
                     /locus_tag="VF874_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175411.1"
                     /translation="MTLEPTNSSRWTVAAALAGTGLALALAFGPAAGTANADVLDDLA
                     QEFSTAAGAGQVSTLLNQS"
     gene            complement(293..3262)
                     /locus_tag="VF874_04695"
     CDS             complement(293..3262)
                     /locus_tag="VF874_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9175412.1"
                     /translation="MVDFSPITRPVGRLIATAQNGLEVIRYGGLETGSVPSPFQIVES
                     TPMYRLRRYFPPDNRNGQTAVGPPVLMVHPMMMSANMWDVTREDGAVGILRNAGIDPW
                     VIDFGSPDRVEGGMERTLADHIVAVSEAIDTVKKTTGQDVHLAGYSQGGMWAYQTAGY
                     RRSGDLASIVAFGSPVDTLAALPMGLPPNLVPAAAEFMADHVFSRIDIPSWLARTGFQ
                     LMDPIKTAKARVDFLRQLHDREALLPREQQRRFLESEGWIAWGGPAISELLKQFIAHN
                     RMMTGGFSIHGELVTLTDIDVPILAVIGEVDDIGQPAAVRGIQRAAPNADVYEYLIRS
                     GHFGLVVGSKAAGQTWPTVAAWVKWLSGEGDMPEGISRMSPQPAEPKESGVPFASRIA
                     HGAGVASEVVFDLARSAGHAVVAANKSMRTLAVETARTLPRLTRLGHINEHTRISLGR
                     IMSEQAANAPEGECLLFDGRVHTYEAVDTRINNVVRGLIEVGVRQGDRVGVLMETRPS
                     ALVAIAALSRLGAVAVLMPPDADLGLAARLGGVREILTDPTNLKAARRLSLQALVLGG
                     GETRDLHLLPNDDDVIDMEQIDPDEVELPGWYRPNPGLAKDIAFVAFTQVGGELVARQ
                     ITNYRWAVSAFGTASTAALSRRDTVYCLTPLHHPSGLLVSLGGAVVGGSRIALSRGLQ
                     ADRFVQEMRQYGVSVVTYTWAMLREVIEDPAFDLKGHHPVRLFIGSGMPTGLWQRVID
                     VFSPAQVVEFFATTDGQAVLANVAGVKIGSEGRPLPGAAQVELGAYEPDDDLILEDDR
                     GFVRRAQTDEVGVLLAHPRGPVDPHAAVKRGVFAPGDTWISTEYLFRRDSDGDFWLMG
                     TRGSVIRTARGAVYDAPINDTVSLMPAVDLAVTYGVDAGDRQVAVTAVTMCPGAALSA
                     ADLSEAMAHLGNDRPDFVHVVPDLALSASYRPTVGALRNAGIPKAGRHAWYLDPETNR
                     YKRLTAPARAEWEQTSH"
     gene            3342..5090
                     /locus_tag="VF874_04700"
     CDS             3342..5090
                     /locus_tag="VF874_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9175413.1"
                     /translation="MANLINAERISVSYGTRTLLDAVSLGVDDGDAIGVVGRNGDGKT
                     TLLTTLAGVRPPDQGRVTHTAGLSIGYLGQTDDFGSDDTVRRLIVGGRPDHVWAGDPN
                     TRAVVQNLLAGVSLDASVGTLSGGERRRVALVAVLLAGHNVLMLDEPTNHLDVEAITW
                     LAGYLSARREQALMVVSHDRWFLDAVCTRTWEVHEGVVDGYDGGYAAYVLARAERMRV
                     AAGADARRRNLMRKELAWLRRGPPARTSKPKFRIQAANALIADEPPARDSLVLQQFAT
                     SRLGKDVFDLRGVRLQVGDRCVLDGVDWSIGPGARIGLVGVNGTGKTSVLRLLAGELA
                     PTGGSVKRGRTLRIGHLSQGLSEITGGDRVLDAVEDQRRVARLAGGREISAATLLGDF
                     GFTGDKLTARLSDLSGGEHRRLQFLRLLLDEPNVLLLDEPTNDLDIDTLTVVEDYLDG
                     WPGTLIVVTHDRYFLERVTDVTYALSGDGRCELLPGGIDQYLAARAESSGAPSSADPG
                     RVESAAARTRRTRKELARIETRLGKLEQRISGLHEEMAAAAHDHVRLGELNAQLGEVL
                     AAKDELEQAWLEAADD"
     gene            complement(5087..6499)
                     /gene="argH"
                     /locus_tag="VF874_04705"
     CDS             complement(5087..6499)
                     /gene="argH"
                     /locus_tag="VF874_04705"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX9175414.1"
                     /translation="MSTNEGTLWGGRFAVGPSPALAALSKSTHFDWVLAPYDINASKA
                     HARILFRAGLLTEDQRDGLLAGLDSLATDVTDGSFGPIVTDEDVHGALERGLIDRVGD
                     DLGGRLRAGRSRNDQIATLFRMWLRDALRRVGAGVLDVIAALTAQAAAHPTAIMPGKT
                     HLQSAQPVLLAHHLLAHAHPLLRDVDRILDCDNRVAVSPYGSGALAGSSLGLDPDAIA
                     EELGFAAASDNSIDATAARDFAAEAAFVLAIIGVDLSRLAEDIILWSTTEFGYVTLHD
                     AWSTGSSIMPQKKNPDIAELTRGKSGRLIGNLTGLLATLKAQPLAYNRDLQEDKEPVF
                     DSVAQLEMLLPAMAGLVSTLRFDVERMAELAPAGYTLATDVAEWLVRQGVPFRVAHEA
                     AGAAVRTAEVRGVGLEDLTDDELAAAHPGLTPEVREVLTVEGSVSARTARGGTAPTQV
                     AKQLADVRSTADQLRNRLRG"
     gene            complement(6496..7695)
                     /locus_tag="VF874_04710"
     CDS             complement(6496..7695)
                     /locus_tag="VF874_04710"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX9175415.1"
                     /translation="MSDRVILAYSGGLDTSVAISWIGKETGREVVAVAIDLGQGGEDM
                     ELVRQRALDCGAVEAVVVDARDEFANEYCLPTIQFNGLYMDRYPLVSAISRPLIAKHL
                     VAAARQHGGSTVAHGCTGKGNDQVRFEVGFASLAPDLEVLAPVRDYAWTREKAIAFAE
                     ENAIPITVTKRSPFSVDQNVWGRAVETGFLEHLWNAPTKDVYDYTEDPTVNWSTPDEV
                     IVGFERGVPVSIDGREVTVLQAIEELNRRAGAQGVGRLDVVEDRLVGIKSREIYEAPG
                     AMVLITAHTELEHITLERELGRFKRTTDQRWAELVYDGLWYSPLKSALEAFVAKTQEH
                     VSGEIRMVLHGGHIAVNGRRSAESLYDFNLATYDEGDSFDQSAARGFVHVHGLSSKIA
                     ARRDLEA"
     gene            complement(7781..8269)
                     /locus_tag="VF874_04715"
     CDS             complement(7781..8269)
                     /locus_tag="VF874_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX9175416.1"
                     /translation="MNAPEISTTRAGRQARIVAILSSRAVRSQSELAAALAAEGIETT
                     QATLSRDLEELGAVKLRGADGGAGAYVVPEDGSPVRGVSGGTERLSRLLGELLVSSDA
                     SGNLAVLRTPPGAAHYLALAIDRSALPYVVGTVAGDDTILVVARDPMTGAELVARFDE
                     LQ"
     gene            complement(8266..9189)
                     /gene="argF"
                     /locus_tag="VF874_04720"
     CDS             complement(8266..9189)
                     /gene="argF"
                     /locus_tag="VF874_04720"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX9175417.1"
                     /translation="MRHFLRDDDLTPAEQAEVLELAAELKKDPFSRRPLDGPRGVAVI
                     FDKNSTRTRFSFELGIAQLGGHAVVVDARSTQLGRDETLEDTARVLSRYVDAIVWRTY
                     GQDRLSAMASVATVPVINALSDDFHPCQVLADLQTIAERKGRLAGLRVAYFGDGANNM
                     AHSLLLGGATAGVHVTIAAPKGFEPDPDVLAAAETRASDTGASVNVTSAPKAAADGAD
                     VLVTDTWTSMGQEDDGQDRVGPFEAFRVDSRLLRYADSDAIVLHCLPAHRGQEITDDV
                     MDGPHSAVWDEAENRLHAQKALLVWLLERTV"
     gene            complement(9186..>9393)
                     /locus_tag="VF874_04725"
     CDS             complement(9186..>9393)
                     /locus_tag="VF874_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9175418.1"
                     /translation="VRGRGLLRGVVLTAPVAKTVETAARDAGFLINAPAPNIVRLAPP
                     LIITEAQIDEFLNALPGVLEKGTA"
BASE COUNT         1471 a         3211 c         3190 g         1521 t
ORIGIN      
        1 cgactggttc agcagcgtcg acacctggcc ggcccccgcc gcggtggaga attcctgggc
       61 cagatcgtcg aggacgtcgg cgttcgccgt gcccgcagcg ggaccgaacg cgagcgccag
      121 ggccaacccg gtgccggcaa gtgcggccgc gacagtccat cgtgagctgt tcgtcggctc
      181 aagcgtcatg cgatccctcc ggaagccgag tgcaaggcgt gcgggtgcca ccgacgggag
      241 cttaacagcg cttgtccggt caggcatcag ttacaggagt ctcttgaagt gactaatggg
      301 acgtctgttc ccactcggcg cgcgccggcg cggtcagccg cttgtagcgg ttggtctccg
      361 gatcgaggta ccaggcgtgc cgccccgcct tggggatacc ggcgttgcgc agagcgccga
      421 ccgtcgggcg atacgaggcg ctcaaggcca aatcggggac gacgtgcacg aagtcaggac
      481 ggtcgttgcc caggtgtgcc atcgcctcgc tcaaatccgc cgccgagagc gccgcaccgg
      541 ggcacatcgt caccgcggtc acggccacct ggcggtcccc ggcgtcgact ccgtacgtca
      601 ccgccagatc gaccgccggc atcaggctga cggtgtcgtt gatcggggcg tcgtagactg
      661 cgccccgggc ggtgcggatc accgagcccc gggtgcccat cagccagaag tcgccatcgc
      721 tgtcgcgccg gaacagatac tcagtggaga tccacgtgtc gccgggagcg aacaccccgc
      781 gcttgaccgc agcatggggg tccaccggcc cgcgcgggtg ggccagcagc acgccgacct
      841 cgtcggtctg cgcccggcgc acgaacccgc ggtcgtcctc gaggatgagg tcgtcgtcgg
      901 gctcgtacgc cccgagttcg acctgcgcag cccccggcag cggcctgccc tcgctgccga
      961 tcttgacgcc cgcgacgttg gccagcacgg cctgcccgtc ggtggtggcg aagaactcca
     1021 cgacctgagc cggcgagaac acgtcgatga cgcgctgcca cagccccgtc ggcatgccgg
     1081 aaccgatgaa cagccgcacc gggtggtggc ccttcaggtc gaaggcgggg tcctcaatga
     1141 cctcgcgcag catcgcccag gtgtacgtca ccaccgagac tccgtactga cgcatctcct
     1201 gcacgaaccg gtccgcctgc agaccgcgcg acaacgcgat ccgggacccg ccgacgacgg
     1261 cgccgccgag gctcaccagc agaccggacg ggtggtgcag cggggtgagg cagtacacgg
     1321 tgtcgcggcg gctgagcgcg gccgtcgacg ccgtgccgaa cgccgacact gcccatcggt
     1381 agttggtgat ctgccgcgcg accagctccc cacccacttg ggtgaaggcc acgaacgcga
     1441 tgtccttcgc caaccccggg ttcggccggt accagccggg cagctcgacc tcgtcggggt
     1501 cgatctgctc catgtcgatc acgtcgtcgt cgttgggcag caggtgcagg tcgcgggtct
     1561 cgccgccgcc gagcaccaga gcctgcagcg agagccggcg ggcagccttg aggttcgtcg
     1621 ggtcggtgag gatctcgcgg acgccgccca gccgcgccgc aaggccgagg tcggcgtcgg
     1681 gcggcatcag caccgcgacc gcgccaagcc gtgacagcgc cgcgatggcc accagcgcgc
     1741 tgggccgggt ctccatcagc accccgaccc ggtcgccctg gcgcacgccg acctcgatca
     1801 ggccgcgcac gacgttgttg atgcgcgtgt ccaccgcctc gtaggtgtgg acacgtccgt
     1861 cgaacagcag gcattcgccc tctggcgcgt tggcggcttg ttccgacatg attcggccca
     1921 gcgagatgcg cgtgtgctcg ttgatatgac cgaggcgagt gagccgcggc agcgtgcggg
     1981 cggtctccac cgccagtgtt cgcattgact tgttcgcggc gacgaccgcg tgccccgccg
     2041 agcgggccaa atcgaacacc acctccgacg ccacgcccgc gccgtgggcg atgcgggacg
     2101 cgaagggcac gccgctctcc ttgggttcgg ccggctgcgg cgacatccgg ctgatcccct
     2161 cgggcatgtc gccctcgccg gacagccact tcacccacgc tgccaccgtc ggccaggtct
     2221 gcccggcagc tttcgagccg acgacgagcc cgaaatggcc gctgcggatg aggtactcgt
     2281 agacatcggc gttgggcgcc gcgcgttgga taccgcgcac ggccgccggc tggccgatgt
     2341 cgtcgacctc accgatgacc gcgaggatcg ggacgtcgat gtcggtgagc gtgacgagtt
     2401 caccgtggat cgagaagcca ccggtcatca tccggttgtg ggcgatgaac tgcttgagca
     2461 actctgagat cgcggggcca ccccaggcga tccacccttc cgactcgagg aagcggcgct
     2521 gctgctcccg cggtaacagc gcctcacggt cgtgcaactg gcggaggaaa tcaacgcgcg
     2581 ccttcgccgt cttgatcggg tccatcaact gaaagccggt gcgcgccaac caacttggga
     2641 tgtcgatgcg actgaacaca tggtcggcca tgaactccgc ggcggcgggc acaaggttgg
     2701 gcggcagacc catcggcaac gccgcgagtg tgtccaccgg cgagccgaat gcgacgatgc
     2761 tggccagatc tccggatcgc cggtagccag ccgtctgata cgcccacatg ccgccttgcg
     2821 agtagccggc caggtgaacg tcttgacccg tggtcttctt gaccgtgtcg attgcctcgc
     2881 tgaccgcgac gatgtggtcg gcgagtgtgc gttccatgcc gccttcgacg cggtcgggcg
     2941 aaccgaagtc gatgacccac gggtcgatgc ccgcgttgcg caggatgccg acggcgccgt
     3001 cttcacgggt gacgtcccac atgtttgcgg acatcatcat cgggtgcacc atcagcacag
     3061 gcggcccgac ggccgtctgc ccgttgcggt tgtcgggcgg gaagtaccgc cgcagccggt
     3121 acatcggagt gctctcgacg atctggaacg gcgagggcac gctgccggtc tccagcccgc
     3181 cgtacctgat cacttcgaga ccgttctgcg cggtggcgat caggcggccc accggcctcg
     3241 tgatcggtga aaagtccacc atcggcgctt gcctccgccc tcgtgcatcc cccaattggc
     3301 cgccgtcatt ctggcacgtc gcgccatatc atcggcgacc gatggccaac ctgatcaatg
     3361 cggagcgcat cagcgtctcc tacggcaccc gcacgttgct cgatgcggtg agcctgggcg
     3421 tggacgacgg cgacgcgatc ggggtcgtag ggcgcaacgg ggacgggaag accacgctgc
     3481 tcacgacgct ggcgggcgtc cgccctcccg accagggccg cgtcacacac accgccggac
     3541 tgtccatcgg ttatctcggc cagaccgacg acttcggcag cgacgacacc gtgcgtcgcc
     3601 tgatcgtcgg tgggcgaccc gaccatgtct gggcgggcga cccgaacacc cgagccgtcg
     3661 tgcagaactt gctggccggt gtctcgctgg atgcctctgt cggcacgctg tccgggggcg
     3721 agcgtcgccg cgtggcgctg gtggcggtgc tgctggcagg ccacaacgtg ttgatgctcg
     3781 acgagccgac gaaccacctc gacgtcgagg cgattacctg gctggcgggt tatctgtccg
     3841 ctcggcgcga gcaagcgctg atggtggtga gccacgaccg ctggtttctc gacgcggtgt
     3901 gcacccgcac atgggaagtg cacgagggtg tcgtcgacgg ctatgacggc ggctacgcgg
     3961 cttatgtgct tgcccgtgcc gagcggatgc gggtggcggc gggcgccgac gcgcggaggc
     4021 gcaacctcat gcgcaaggag ctggcgtggt tgcggcgtgg cccacccgcc cgcacgtcga
     4081 agccgaagtt tcggatccag gcggccaacg cgctgatcgc cgacgagcct ccggcacgcg
     4141 attcgcttgt gctgcaacag tttgcgacga gccggctggg caaagatgtg ttcgacctgc
     4201 gcggcgttcg gctgcaggtc ggtgaccgtt gcgtcctcga tggcgtggac tggtcgatcg
     4261 gtccgggcgc gcgcatcggg ttggtggggg tcaacggcac gggcaagacg tcggtgttgc
     4321 ggctgctcgc tggtgaattg gcgccgaccg gtggaagcgt caagcgcggc aggacgttac
     4381 gcatcgggca cttgagccag gggctgagcg agatcaccgg tggtgaccgc gtgctggacg
     4441 cggtcgaaga ccagcgccgg gtggcgcgac tcgcaggggg ccgggaaatc agcgccgcga
     4501 cactgctcgg ggacttcggt ttcaccggcg acaagttgac tgcgcggctt tccgacctgt
     4561 ccggcggcga gcaccggcgg ctgcagttct tgcggctgct gctcgacgaa ccgaacgtgc
     4621 tgcttctcga cgagccgacc aacgacctcg acatcgacac gctgaccgtg gtcgaggact
     4681 atctcgacgg ctggcccggc acgttgatcg tcgtcacgca cgaccgctac ttcctcgaac
     4741 gagtcaccga cgtcacgtac gcactgtcgg gtgacggccg ctgcgaactg ctgcccggag
     4801 gcatcgatca gtatctggcg gcgcgggcgg agtcttcggg cgcgccgtct tccgccgatc
     4861 cgggccgcgt cgaatcagca gctgcccgga ctcgccgcac cagaaaagag ttggcccgca
     4921 tcgaaacccg gctgggaaag ctagagcagc ggatcagcgg gctgcacgag gagatggcgg
     4981 ccgccgcgca cgaccacgtg cggctgggtg agctgaacgc gcagttaggc gaggtgctgg
     5041 ccgcgaagga cgagttggaa caggcctggc tcgaggcggc agacgactag cccctcagtc
     5101 ggtttcgcag ctggtcggcg gtgctgcgta cgtcggcaag ctgtttggcc acttgcgtcg
     5161 gcgccgtgcc gccacgggcc gtccgagccg acaccgatcc ctcgaccgtc agcacctcac
     5221 gaacctccgg cgtcaagccc ggatgcgcgg cggcgagttc gtcgtcggtg agatcctcca
     5281 acccgacgcc gcggacctcg gccgtgcgga ccgccgcacc cgcagcttcg tgcgcgaccc
     5341 ggaacggcac gccctgacgc accagccatt cggcgacgtc cgtggccaag gtgtaccccg
     5401 ccggtgccag ctcggccatc cgctcgacgt cgaaccgcaa ggtggacacc agccccgcca
     5461 tcgccggcag cagcatctcc agttgcgcca cggagtcgaa caccggctcc ttgtcttcct
     5521 gcaggtcgcg gttgtacgcc agtggctgag ccttcagcgt ggcgaggaga ccggtcaggt
     5581 tgccgatcag ccggcccgac tttcctcggg tcaactcggc gatgtcgggg ttcttcttct
     5641 gcggcatgat ggagctgccc gtagaccacg cgtcgtgcaa ggtgacgtac ccgaattccg
     5701 ttgtgctcca aaggatgatg tcctcagcca atcgagacag gtcgacgccg atgatcgcga
     5761 gcacgaacgc ggcctcggcg gcgaagtcgc gggcggcggt ggcgtcgatc gagttgtctg
     5821 atgccgcagc gaatcccagc tcctcggcga tcgcgtcggg gtcaagcccc agcgaagacc
     5881 ctgccagcgc gcccgacccg tacggcgaga cggccacccg gttgtcgcag tcgaggatgc
     5941 ggtcgacgtc ccgcagtagc ggatgggcgt gcgccagcag gtgatgcgcc aacagcaccg
     6001 gctgagccga ctgcaggtgg gtcttgccgg gcatgatcgc ggtcgggtgg gcggccgcct
     6061 gggcggtcag cgcggcgatc acgtccaaca cgccggcccc cacccgtctc agcgcgtcgc
     6121 gcagccacat ccggaatagc gtggcgatct gatcatttcg cgagcggccg gcgcggaggc
     6181 ggcccccgag gtcgtcgccg acccggtcga tcagtccacg ctccagcgcg ccgtggacgt
     6241 cctcgtcggt gacgatcggg ccgaagctgc cgtctgtgac gtctgttgca aggctgtcca
     6301 ggcccgccag cagtccgtcg cgctggtctt cggtcagcag gccggcccga aataggatgc
     6361 gggcgtgggc ctttgacgcg ttgatgtcgt aaggggcgag tacccagtcg aagtgcgtcg
     6421 acttgctgag tgcggccagc gccggcgacg ggccaacggc gaaccggccg ccccacagcg
     6481 tgccctcgtt agtgctcacg cctctagatc ccggcgggcg gcgatctttg acgacaggcc
     6541 gtgcacgtgg acgaaacctc gcgcagcgga ctggtcgaag gagtcaccct cgtcgtaagt
     6601 ggccaggttg aagtcgtaaa gcgattcggc actgcgccgc ccgttgaccg caatatgccc
     6661 gccgtgcaac accattcgga tctcgccgga gacgtgctcc tgcgtcttgg cgacgaacgc
     6721 ctccagcgcc gacttcagcg gcgagtacca gagcccgtcg tacaccaact ccgcccagcg
     6781 ctggtcggtg gtgcgcttga accggccgag ttcgcgctcc agcgtgatgt gctcgagctc
     6841 ggtgtgcgcg gtgatcaaca ccatcgcgcc cggtgcctcg tagatttccc ggctcttgat
     6901 gccgacgagg cgatcctcga cgacgtcgag ccggccgacg ccttgcgcgc cggcacgccg
     6961 attcagctcc tcgatggcct gcagcacggt gacctcacgg ccgtcgatcg aaaccgggac
     7021 gccacgttcg aagccgacga tcacctcgtc gggcgtggac cagttgacgg tcggatcttc
     7081 ggtgtagtcg tagacgtcct tggtgggagc gttccacaag tgttcgagga agccggtttc
     7141 caccgccctg ccccagacat tctggtcgac ggagaacggc gaccgcttgg tcacggtaat
     7201 cggtatcgcg ttctcctcgg cgaatgcgat ggccttctcc cgcgtccacg cgtagtcacg
     7261 caccggtgcc agcacctcga gatccggtgc cagcgaggcg aagcccacct cgaaccgcac
     7321 ctggtcgttg cctttgccgg tgcagccgtg cgcgacggtg ctgccgccgt gctgccgcgc
     7381 agcggcgacg aggtgcttgg cgatcagcgg ccggctgatc gcggacacca gcggatagcg
     7441 gtccatgtag aggccgttga actggatggt gggcaggcag tactcgttcg cgaactcgtc
     7501 acgcgcatcg accacgacgg cctccaccgc gccgcagtcg agggcgcgct gccgcaccag
     7561 ttccatgtcc tcgccaccct ggccgaggtc gatcgccacg gccaccacct cgcggccggt
     7621 ctcttttccg atccagctga tcgccaccga cgtgtccaaa ccgccggagt acgccaggat
     7681 gacgcgatcg gacatgggct atctcctctt tagtgttggc tcttcgccca agaggctcat
     7741 cgccgcgcaa gcgctcatcg gtaggctatc tccttgccaa ttactgcagc tcgtcgaacc
     7801 gggcgaccag ttcggcgccg gtcatcgggt cgcgcgcgac caccagtatt gtgtcgtcgc
     7861 cggcgacggt gccgacaaca taagggagtg cggaccggtc gatcgccaag gccagatagt
     7921 gcgcggcgcc gggtggagtg cgcagcaccg ccaggttgcc gctggcgtcg ctggacacca
     7981 gcagttcgcc caagaggcgc gaaagccgtt ccgtgccacc ggaaacgccg cgcacggggc
     8041 tgccgtcctc cgggaccacg tatgcgccgg cgccgccgtc tgccccgcgc agcttgacgg
     8101 caccgagttc ctcgagatcc cgcgagagcg tcgcctgcgt ggtctcgatg ccctcggcgg
     8161 caagggccgc ggccaactcg ctctggctgc gcaccgcccg cgacgacagg atcgcgacaa
     8221 tgcgtgcctg ccgtccggcg cgggtggtgc tgatttccgg tgcgttcata ccgtccgctc
     8281 cagcagccac accagcagcg ccttctgggc gtgcagccga ttctcggcct cgtcccacac
     8341 cgcgctgtgc gggccgtcca tcacgtcgtc ggtgatctct tggccgcggt gcgccggaag
     8401 gcaatgcagc acaatggcat ccgagtcagc gtaccgaagc agccggctgt cgacacggaa
     8461 tgcctcgaac ggtccgacgc ggtcttgccc atcgtcctct tggcccatcg acgtccaggt
     8521 gtcggtgacc aacacgtcgg cgccgtcagc ggcggctttc ggcgcggagg tgacgttgac
     8581 gctcgcgccg gtgtcggagg cacgggtttc agcggcggcg agcacgtcgg ggtccggctc
     8641 gaaacctttc ggcgccgcga tcgtgacgtg cactcccgct gtcgcgccgc cgagaagcag
     8701 cgaatgcgcc atgttgttgg ccccgtcgcc gaaataggcc acgcgcagac ccgccagccg
     8761 acccttgcgc tcggcgatcg tctgcaggtc ggcgagcact tggcacgggt gaaagtcgtc
     8821 ggacagtgcg ttgatcaccg gcaccgtcgc caccgacgcc atcgcgctca gccggtcctg
     8881 gccgtacgtc cgccacacga tcgcgtcgac gtagcgggac aacacccgag cggtgtcctc
     8941 cagcgtttcg tcgcgtccca gttgagtgct gcgcgcgtcg acgacgacgg cgtgcccgcc
     9001 gagttgggcg atgcccagtt cgaaggaaaa ccttgtgcgg gtggagtttt tgtcgaagat
     9061 caccgcgacg ccgcgcgggc cgtcgagcgg acggcggctg aacggatcct tcttcagctc
     9121 tgccgccagc tccagtacct cggcctgctc ggccggtgtc agatcgtcgt cacgcagaaa
     9181 gtgcctcatg ctgttccctt ttcgagtacc ccaggcaatg cgttgaggaa ctcgtcgatc
     9241 tgcgcttcgg tgatgatcaa cggcggcgcg agccgcacga tgttcggagc gggcgcgttg
     9301 atcaggaaac cggcgtcgcg ggcggcagtc tcgacggtct tggccaccgg tgccgtcagg
     9361 accacaccac gcagcagacc gcgtccccgg aca
//
LOCUS       DASWBF010000140         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1199763,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000140 DASWBF010000000
VERSION     DASWBF010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199763"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VF874_04730"
     CDS             <1..564
                     /locus_tag="VF874_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175419.1"
                     /translation="RANVDRFGGDREFVAIAGCSAGGHLAALTGLTHDDTDFHVDLAD
                     DADTSVDAVVGIYGRYDWEDRSTPERDRFVDFLERVVVKKRLAKHPELFRKASPIARV
                     RADAPPFLVVHGSADNVIPVAQAREFVERLRAASRSTVGYLELPGASHGFDMTDGSRT
                     RSAATVIGLFLDEIHRGHLLSRAKEVI"
     gene            604..2037
                     /locus_tag="VF874_04735"
     CDS             604..2037
                     /locus_tag="VF874_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9175420.1"
                     /translation="MKRLNGRDALLLYSETPNIHTHTLKIGVIDATDFDGEFTFELFR
                     RTLRRRLHLLEPLRYKLVDIPLKLHHPMWMEKSEIDLSYHLRRVRVRRPGGRRELDEL
                     IGEIASTPLDRGLPLWQMYFAEGMADNRFAVIGKVHHALADGVASANMMARAMDLRGT
                     IQDERDLYATNAPPSTGELLKEAWRDHVRQFRKLPRLARETAAGVGRVRKRGKERGQQ
                     PEMAKSFSPPPTFINHVVSPGRRFATATLALSDVKQTSKHLGITINDLVLATAAGALR
                     ELLLRYDGRADAPLIGSVPVSLDTSPDRLTGNEFFGLNVSLPVHVDDPIERVRLTALA
                     AGIAKEDFGLLGPDAVSQWAAYLPPGAAHAAFKWLSKREAQSRLLNVPISNVAGPREP
                     GSIGGATVSEIYSAGPLIAGSGMNITVWSYVDQLNISVLTDDLTLDDPHEATDAIIRS
                     FTEIRSAAGFSSELTPVHTAMAPARVS"
     gene            complement(2039..3304)
                     /locus_tag="VF874_04740"
     CDS             complement(2039..3304)
                     /locus_tag="VF874_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175421.1"
                     /translation="MADSSYRRLRRRRPLLRSIVELINALNGLQPLGRSGYRTLPAFW
                     FGWHTSELPTVYLSGSVFDAIRRGRRGDFGGLRGKLALVVTAAAWTVLAVIYRRNVTT
                     PGPVLTAALRDGLGADYADVVATLPSAPSLTGRPAGALRTSQIRRRYVSRDSVVHYGP
                     HRADVADVWRKRDLPREGKAPVLVQIPGGGWVIGMRRPQAYPLLSHMADRGWVCVSLG
                     YRVSPVHTWPDHIVDVKRGLAWVREHIAEFGGDPDFIAITGGSAGGHLASLAALTPND
                     PAWQPGFEDADTSVVAAVPIYGRYDWYSTDGPGRRDFVEFLAKFVVKKSFKEHRQVFV
                     DASSIARVHSDAPPFFILHGQNDTIIPVEEARDFVREFRDVAKRPVLYAELPHAQHAF
                     EMYGSPRAYQTAEAVAHFLSWVYAKTRGS"
BASE COUNT          562 a         1123 c         1124 g          502 t
ORIGIN      
        1 cgcgctaacg tcgaccggtt cggcggcgac cgcgaattcg ttgcgatcgc aggctgttcg
       61 gccggcggtc acctcgcggc gctgaccggc ctgacgcacg acgacaccga cttccacgtc
      121 gatctcgccg acgacgccga cacgtccgtc gacgcggtgg tgggcatcta cggccggtac
      181 gactgggagg acaggtccac cccggaacgt gacaggttcg tcgacttcct ggagcgggtc
      241 gtcgtcaaga agcggctggc caaacatccg gagctgttcc gcaaggcgtc accgatcgcg
      301 cgggtgcgtg ccgacgcgcc gccgttcctg gtcgtgcacg gctccgcgga caatgtgatc
      361 ccggtcgcgc aggcccgcga gttcgtggag aggctgcggg cggcgtcgcg ctcgacggtc
      421 ggctacctgg agctgcccgg ggcgagccac ggcttcgaca tgaccgacgg gtcgcgcacc
      481 cggtccgcgg cgacggtcat agggttgttc ctcgacgaga tccatcgagg ccacttgctg
      541 agcagggcaa aagaggttat atagcgttca gcacccgtcg ggtcgtcctt gaagaggtga
      601 ggagtgaaac ggctcaacgg ccgggatgcc ttgttgctgt acagcgagac acccaacatc
      661 cacacgcaca cgttgaagat cggcgtgatc gacgccaccg acttcgacgg tgagttcacg
      721 ttcgagttgt tccggcgcac cctgcgccgc cgcctgcacc tgctggagcc gctgcgctac
      781 aagctcgtcg acatcccgct gaagctgcac cacccgatgt ggatggagaa gtcggagatc
      841 gacttgagtt accacctgcg cagggtgcgg gtgcgtcgtc ccggcgggcg tcgcgaacta
      901 gacgagctga tcggcgagat cgcgagcacg ccgctggacc gcggcctccc gctgtggcag
      961 atgtacttcg ccgagggcat ggccgacaac cggttcgcgg tgatcgggaa ggtgcaccac
     1021 gcgctggccg acggggtcgc gtcggcgaac atgatggcca gggccatgga cctgagaggc
     1081 acgatccaag acgagcgcga cctctacgcc accaacgccc cgccgtcgac aggcgagttg
     1141 ctgaaagagg cttggcgaga ccacgtccgc cagttccgca agctgccgcg gctggccagg
     1201 gagacggccg cgggcgtcgg ccgggtgcgc aagcgcggca aggagcgcgg ccagcagccc
     1261 gaaatggcca agtcgttctc gccgccgccg acgttcatca accacgtggt gtcgccgggg
     1321 cgccggttcg cgacagccac actggcgctg tcggacgtta agcagaccag caagcacctc
     1381 ggcatcacga tcaacgacct ggtgctggcc accgcggccg gcgcgctgcg ggagttgctg
     1441 ctgcgctacg acggccgtgc cgacgcgccg ctgatcgggt cggtacccgt ctcgctcgac
     1501 acatccccgg accggctgac cggaaacgag ttcttcgggc tcaacgtctc gctgccggtg
     1561 cacgtcgacg acccgatcga aagagtgcgg ctgaccgcgc tcgcggccgg gatcgccaag
     1621 gaggacttcg gtttgctcgg gcccgacgcg gtgtcgcagt gggcggcgta cctgccgccc
     1681 ggcgcggcgc acgcggcgtt caagtggctg tccaagcgcg aggcgcagag tcggctgctc
     1741 aacgtgccga tctccaacgt cgcgggtccg cgggagccgg gcagcatcgg cggcgcgacg
     1801 gtcagcgaaa tctattccgc cggaccgctg atcgcgggca gcggcatgaa catcaccgtg
     1861 tggagctacg tcgaccagct caacatctcg gtgctcaccg acgacctcac gctcgacgac
     1921 ccacacgaag ccaccgacgc gattatccgc tcgttcactg aaatccgttc cgctgcaggg
     1981 ttttccagcg aactcacccc ggtgcacacg gccatggcgc cggcgcgggt gagctgattc
     2041 agctgccgcg ggttttcgcg tacacccacg acaagaaatg cgcgacggct tcggcggtct
     2101 gatacgcgcg tggcgagccg tacatctcga atgcgtgctg tgcatgcggc aattcggcgt
     2161 agagcaccgg acgctttgcg acgtcgcgga attcgcgcac gaaatcccga gcctcttcga
     2221 ccgggatgat cgtgtcgttc tgtccgtgca ggatgaagaa cggcggtgca tcggagtgga
     2281 cgcgggcgat cgacgatgcg tcgacgaaca cctgccggtg ctccttgaag ctcttcttca
     2341 ccacgaactt cgccaggaac tcgacgaagt cgcgccgccc cgggccgtcg gtggaatacc
     2401 agtcgtagcg cccgtagatc ggcaccgccg ccactaccga ggtgtcggcg tcttcgaagc
     2461 cgggctgcca tgccgggtcg ttcggcgtca gcgcggccag tgaggcgagg tggccgccgg
     2521 ccgagccgcc cgtgatcgcg atgaagtccg ggtcgccgcc gaactcggcg atgtgctccc
     2581 tgacccacgc caggccacgt ttgacatcga cgatgtggtc gggccaggtg tgcacagggc
     2641 tgacgcggta gccgagcgac acgcacaccc agccgcggtc ggccatgtgg ctcagcagcg
     2701 ggtaggcctg cggccggcgc atgccgatca cccagccgcc acctggaatc tgcaccagca
     2761 caggggcttt cccctcacgc ggcaagtccc gcttgcgcca cacgtccgcc acgtccgccc
     2821 ggtgcgggcc gtagtggacg acgctgtctc gggatacgta gcggcgacgg atttggctgg
     2881 tgcgcagcgc gccggcgggc cgccccgtca acgacggcgc cgagggcagc gtcgccacca
     2941 cgtcggcgta gtccgcgccg agcccgtcgc gcagcgccgc cgtaagcacc ggcccaggtg
     3001 tcgtcacgtt gcgtcggtag atcaccgcca acaccgtcca cgccgcggcg gtcaccacca
     3061 gcgcgagctt gccgcgcaac ccgccgaagt cgccacggcg accgcgtcgg atggcgtcga
     3121 acacggaacc gctcaggtac accgtcggca attccgaggt gtgccagccg aaccagaatg
     3181 ccggtagcgt tcggtagccg ctacggccga gcggctgtaa cccgttgagc gcgttgatca
     3241 attcgacaat cgaacgaagc agcgggcgcc gccgccgcaa gcgccgatac gaggagtcgg
     3301 ccatcgctca a
//
LOCUS       DASWBF010000141        12060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_14286, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000141 DASWBF010000000
VERSION     DASWBF010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12060
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14286"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VF874_04745"
     CDS             <1..726
                     /locus_tag="VF874_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014986063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9175422.1"
                     /translation="IVWDAHVGGIPVCLLGIESHTVARRGFVPAGGPSSYTSGTLFPQ
                     SSRKIARAINGASGNRPLVVLANLSGFDGSPESMRHWQLEYGAEIGRAVTNFRGPIVF
                     VVVSRYHGGAFVVFSKRLNERLEIAAVEGSFASVIGGPPAAATVFARDVDARTQQDPQ
                     VRESAERVREATGSDASTARARLAEVTAAVRSAKLSEVAEEFDRIHTVQRALAVGSVD
                     RIIAAKALRPYVVDALKRGMSAV"
     gene            complement(715..1458)
                     /locus_tag="VF874_04750"
     CDS             complement(715..1458)
                     /locus_tag="VF874_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX9175423.1"
                     /translation="MERHRWLALGEDALPDDEHWLSPGEAEVLARLRYTKRRTEYLLR
                     RLVTKHAVASVTGRSIDPADLAGIEVRNAPSGAPFVCVDGDPVDFGVSISDRAGWAVC
                     VTCPTESPTVGCDLELVEPRTPGFVADFLTSAEQRLVAAHHEPDAAANLIWSAKESAL
                     KVLQTGLRRDTRSVEVMVDEPGEDGWGALTVRAVEGPVFPGWWRRDGRFVLTTAAEVT
                     HPPPAPLGDPGVLTLAEPRHSWVDRPLYR"
     gene            1532..2503
                     /locus_tag="VF874_04755"
     CDS             1532..2503
                     /locus_tag="VF874_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9175424.1"
                     /translation="MTAFVTGGSGFIGGRLIERLVADGVGVRALARSDRAAAAVEARG
                     AEAVRGDLADVDAITAGAQGCDTAYHLAANVEMWGAWDDFVRDNVTGTQNVLAATKAA
                     GVARFVHCGTEAALIAGEPLRNVDETAPLRPDSPAPYAATKARAEQEVRAANRDGFET
                     VVVRPRFVWGAGDTTLLPSMVEMVKSGRFAWIGGGKQLTDVTHVDNVVEGLLLGARNG
                     RGGDAYFVTDGEPVIFREFVSALLETQGVAAPSRSIPTWVAGPLAAACEGLWRATPLP
                     GQPPLTRFTYWVSSQECTIDIGKARRELGYRPIKGRAAGLAELGRIS"
     gene            complement(2496..3170)
                     /locus_tag="VF874_04760"
     CDS             complement(2496..3170)
                     /locus_tag="VF874_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175425.1"
                     /translation="MNEVDTGAPVRGSRMRIPRSRGAASGFLLILLGVWGALVPFVGP
                     YFDFAWGSNQEWMWTAGRGWLEVLPGAATVLGGLLLLTSRNRATAMFGGWITVLAGAW
                     FVVGRAVAEAARIGDVGRPLASTDAKFALLDLTYFSGLGTVIVFLGALVLGRLSVRTL
                     RDVQYAQRLVAATEPAPAPVPEATTERTERTEPVAEPATEADEPRRRTRGGLFRRHRE
                     RTTAAS"
     gene            3263..5098
                     /locus_tag="VF874_04765"
     CDS             3263..5098
                     /locus_tag="VF874_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010839928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9175426.1"
                     /translation="MSASIRHSGPVEQLVRLRAQIDDLLCRLSEAEATWQAWIAGTAP
                     ENRFSAGNLVHYWALRQVDLRQMQSELASVGLSSLGRSEPHVQASLRLVASAIAAMLG
                     SGWWPREDVAGRVDEGAKLLRQNAVDLLGQLPAGRAARIMVTLPSEAATDSSLVCGLV
                     EAGMNVARINCAHDDPRAWRAMADNVRAAAATSDTSCLIAMDLGGPKLRTGPLEAGPC
                     VVRLQPRRDAWGQVLAPARGWITSAQNPVEPPEAGTVTLPVPDDWLTRRRTGDVITLT
                     DTRGSLRRLEITSAPGGFVVTCNRTTYLATGTKLGIKGVDDVTEVGELAPTEQSIRLR
                     AGDILHLTRDCSPAPVDARAIPRIGCTLPEVFDHAQVGEAVFFDDGKLHGRIISVDPG
                     TLAVRIDHPPQGRAKLKAGKGINLPDTTLPISALTRKDLDDLETVVEIADLVQVSFAR
                     YPSDVEQLIDELARLSDDRLGIVLKIETREAFERLPQLLLTAMRRRRVGVMIARGDLA
                     VECGYERMAEVQEEILWLCEAAHLPVIWATQVLEQLAKTGVPSRAEISDAAMGERAEC
                     VMLNKGPYIDDAVVALDNILRRMTSHHYKKNALLRTLHAWQQAAH"
     gene            complement(5092..5478)
                     /locus_tag="VF874_04770"
     CDS             complement(5092..5478)
                     /locus_tag="VF874_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEX9175427.1"
                     /translation="MAVAALVFAALAALVHVYIWVLESFLWTTPRTRAVFGTSEEEAQ
                     TSKLLALNQGFYNLFLAVITAVGIAFFGTGCTAVGSALIFAGTGSMALAAVVLAVSSP
                     DKARSALVQGLFPVAAIVLLVLHWSQ"
     gene            complement(5480..5698)
                     /locus_tag="VF874_04775"
     CDS             complement(5480..5698)
                     /locus_tag="VF874_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175428.1"
                     /translation="MLDSATIRFGGAKVSAALGVLGVLPLVHTWIPLPAALSSLVWIF
                     LIGLVMLVAPSGVRATPESVDDSAGTEA"
     gene            complement(5719..6753)
                     /gene="ruvB"
                     /locus_tag="VF874_04780"
     CDS             complement(5719..6753)
                     /gene="ruvB"
                     /locus_tag="VF874_04780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX9175429.1"
                     /translation="MDEADEREVSAALTVGESDVDASLRPRSLDEFIGQPRVREQLQL
                     VIEGAKNRGGTPDHILLSGPPGLGKTSLAMIIAHELGSSLRLTSGPALERAGDLAAML
                     SNLVEHDVLFIDEIHRIARPAEEMLYLAMEDFRVDVVVGKGPGATSIPLEVAPFTLVG
                     ATTRSGALTGPLRDRFGFTAHMDFYEPAELEQVLRRSAGILGIEVVADAGAEIARRSR
                     GTPRIANRLLRRVRDYAEVRADGVITRDVAKTALAVYDVDELGLDRLDRAVLTALIRS
                     FGGGPVGVSTLAVAVGEEATTVEEVCEPFLVRAGMIARTPRGRVATALAWRHLGIPPP
                     SGLGQAGLFD"
     gene            complement(6768..7364)
                     /gene="ruvA"
                     /locus_tag="VF874_04785"
     CDS             complement(6768..7364)
                     /gene="ruvA"
                     /locus_tag="VF874_04785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9175430.1"
                     /translation="MIASIRGEVLDVALDHVVIEACGVGYRVNATPSTLATLRRGSEA
                     RLVTAMIVREDSMTLYGFADSDTRDLFQTLLSVSGVGPRLAMATLAVHEAPALRQALA
                     DGDVTALTRVPGIGKRSAERMVLELRDKVGPARPSTSGAPATNGHAVRGPVVEALVGL
                     GFAAKQAEEATDRVLSGENGEATTSSALRAALSLLGRK"
     gene            complement(7361..7915)
                     /gene="ruvC"
                     /locus_tag="VF874_04790"
     CDS             complement(7361..7915)
                     /gene="ruvC"
                     /locus_tag="VF874_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9175431.1"
                     /translation="MRVMGVDPGLTRCGLSLIESSGGRHVTALDVDVVRTPTDMPLHR
                     RLLAISDVAEHWMDTHQPDVIAIERVFSQQNVSTVMGTAQAGGVIALAAARRGIDVHF
                     HTPSEVKAAVTGSGGAGKSQVTTMVTKILALQTKPAPADAADALALAICHCWRAPMLA
                     RMAQAEALSAEQRKKYEAKVKAAR"
     gene            8272..8670
                     /gene="tnpA"
                     /locus_tag="VF874_04795"
     CDS             8272..8670
                     /gene="tnpA"
                     /locus_tag="VF874_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX9175432.1"
                     /translation="MGVTLRTNANIAFQCAFHVVWCSKYRRAVIGGRMEQRLKQIIAE
                     VVEEKGAWLVELETMPDHVHLLVEVDPQFGVHKLVKAIKGRSSRALREEFPWLRSRLP
                     SLWTNSYFVATVGRAPLSVIKRYVETQKSR"
     gene            8678..9826
                     /locus_tag="VF874_04800"
     CDS             8678..9826
                     /locus_tag="VF874_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9175433.1"
                     /translation="MLTGRRFRVEFTAEQAEYADRIASVCRAVWNTGLEQRREYRRRS
                     GWMNYREQAGELAEAKAEHRWLAEVPGHCLQQTLMDLDKACRAHGTFRVRWRSGRRWR
                     PSFRFPEGNKMVVKQLNGRHARVKLPKLGWVRFRLSRPHDDVVLRSATLTRDGRHWFV
                     SFLVDDGLQTPEVHTAPTAAVGVDRGVVTALATSDGELFDRQFLTAGEQVRVVRLQRK
                     LSRAARRGRNRDKVRSALAEIREREARRRLDFCAQTAHRLAAANAVVVLENLKTKQMT
                     RSAKGTLEQPGTNVRAKSGLNRAILAKGWHQFALALSSAARYSGTRVVAVPAAFTSQR
                     CSACGHLDPKSRESQAVFRCTSCQHGPVHADVNAAKNLLAIGLAATAC"
     gene            10116..>12060
                     /locus_tag="VF874_04805"
     CDS             10116..>12060
                     /locus_tag="VF874_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9175434.1"
                     /translation="MTAGGVRTVRSFCRVCTSVCGILVDVSGDEVIRVRGDREHPLSH
                     GYTCPKGRALPQMHHHPERFERPLIRTDGGLQPTTWDACLDDLCVKLRDIIDRHGPEA
                     VGILFGTGVGMDAAGYRMAQGLHAAIGTPAKFSPLTIDGTAKVLISELMGGSSALTGR
                     PDYDGATLVMLIGSNPVVSHGHTVGMPNPTGAIRDLTKRAQVWVVDPRHTETARLATR
                     HLAPRPGTDYAVLAFLVREVLRDGVEVLVQGVDELAAAVEPFTLAHAAEVADVSRADL
                     SELLAAVRRAGRVAIDTGTGVTMSASANVTQWLSWALLILTDSMNRPGGYWFHPGFAY
                     QLEAFELPISPPEGTFGPGPRSRPETQAFLREWPCAVLPDEIMAGNIRAFLNLGGSLV
                     TAFPDAGSLVPALKQLDVFATTEILPNETTALSTHVLPTKDQLERPDVTLWDILSPRV
                     CAQHTSAVVDAVGERRSMWWVLAEIGRRLGHQLADTSLSDDAMLASLSGGGRCSFEEL
                     AASGYVETPRELPAPWVERHIGRLGGWRLAPPLLVGQLAALREPAPLMLVPRRQRRRL
                     NSQLEFLGESAEVLLHPDDARAAGVVDGEHVIVRSQHGELTGIAKVEPSIRRGAVSVP
                     HGHAEANVNLLTSKDDIDLVTGMT"
BASE COUNT         1930 a         4101 c         4153 g         1876 t
ORIGIN      
        1 atcgtgtggg acgcgcacgt cggcggcatc ccggtgtgcc tgctgggcat cgagtcccac
       61 accgtggcgc ggcgcgggtt cgtgccggcc ggcggcccgt cgtcgtacac ctcgggcacg
      121 ctgtttccgc agtcgtcgcg caagatcgcg cgcgcgatca acggcgccag cggcaaccgc
      181 ccgctcgtcg tgctggcgaa cctgtccggc ttcgacggct caccggagtc catgcgtcac
      241 tggcagctgg agtacggcgc cgagatcggc cgcgcggtca ccaacttccg cggcccgatc
      301 gtgttcgtcg tggtgtcccg ataccacggc ggggcgttcg tggtgttctc caagaggctc
      361 aacgagcgac tcgaaatcgc cgccgtcgag ggctctttcg catcggtgat cggtgggccg
      421 ccggccgcgg ccacggtgtt cgcccgtgac gtggacgccc gcacccagca ggacccgcag
      481 gtgcgcgagt ccgccgagcg ggttcgggag gcgacgggtt cggacgccag cacggcacgg
      541 gcccggctcg ccgaggtcac cgcggcggtg cgctcggcga agctgtccga agtggccgag
      601 gagttcgacc ggatccacac cgtgcagcgg gcgctggcgg tcgggtcggt cgaccggatc
      661 atcgctgcca aggcgctgcg accctacgtc gtcgacgccc tcaagcgggg aatgtcagcg
      721 gtatagcggg cggtcaaccc aggagtgccg cggttccgcg agagtgagga cgccggggtc
      781 gccgagcgga gccggcggcg ggtgcgtcac ctcggctgcc gtcgtcagca cgaaccggcc
      841 gtcgcgtcgc caccagcccg gaaacaccgg cccttcgact gcgcgcaccg tcagcgcgcc
      901 ccagccgtcc tcgcccggct cgtcgaccat cacttcgaca ctgcgggtgt cgcggcgcag
      961 gccggtctgc agcaccttca gcgcgctctc cttggccgac cagatcaggt tcgcggccgc
     1021 gtcgggctca tggtgggcgg ccaccaggcg ttgctcggcg gaggtcagaa agtcggcgac
     1081 gaaacccggc gttcgcggct ccaccagctc caaatcgcaa ccgaccgtgg gcgactcggt
     1141 cgggcacgtc acgcacaccg cccagccggc ccggtcgctg atcgacactc cgaagtcgac
     1201 ggggtcgccg tcgacgcaca cgaacggcgc gccgctgggg gcgttgcgca cctcgatgcc
     1261 ggccaagtcc gccgggtcga tgctgcggcc ggtgaccgac gcgacggcat gcttggtgac
     1321 cagccggcgc agcaggtact cggtgcgccg cttggtgtag cgcagccggg ccaggacttc
     1381 cgcctcgccg ggcgacagcc agtgctcgtc gtcgggcagc gcgtcctcac cgagggccag
     1441 ccaccggtgc ctctccacgc gaaatagcat tccaggtcgt gaatttccag gaatcgcaac
     1501 cctggcttct ctttcggcgt agagaagagg cgtgacggcg ttcgtgaccg gcggctcagg
     1561 gttcatcggc ggccgcctga tcgagcggct cgtcgccgac ggtgtcgggg tgcgggcgct
     1621 ggcccggtcg gaccgcgcgg ccgctgccgt cgaggcgcgc ggtgccgagg cggtccgcgg
     1681 cgatctggcc gacgtcgacg cgatcacggc gggcgctcag ggctgtgaca ccgcctacca
     1741 cctcgcggcc aacgtcgaaa tgtggggcgc ctgggacgat ttcgtccgcg acaacgtcac
     1801 cgggacgcag aacgtcctcg cggccacgaa ggcggccggt gttgcgcgct tcgtccactg
     1861 cggcaccgag gcggccctga tcgccggcga gccgctgcgc aacgtcgacg agaccgcacc
     1921 gcttcggccg gactccccgg cgccgtatgc ggcgacgaag gcccgcgccg agcaggaggt
     1981 cagggccgcg aaccgcgacg gattcgagac cgtggtcgtc cggccgcggt tcgtctgggg
     2041 tgccggtgac acgacgctgc tgccatcgat ggtggagatg gtcaagtccg gcaggttcgc
     2101 gtggatcggc ggcggcaagc agctcaccga cgttacccac gtcgacaacg tcgtcgaagg
     2161 actgctgctc ggcgcgcgca acgggcgtgg cggtgacgcc tacttcgtca ccgacggcga
     2221 gccggtcatc ttccgcgagt tcgtctccgc gctgctcgag acgcagggag tcgccgcgcc
     2281 cagccgctcg atcccgacgt gggtggccgg tcccctcgcg gccgcctgcg aggggctctg
     2341 gcgtgccacc ccgctccccg gccagccgcc gctgacccga ttcacctact gggtctcctc
     2401 gcaagaatgc acgatcgaca tcggcaaggc ccgccgcgaa ctcggctacc ggcccatcaa
     2461 gggacgggcc gcagggctcg ctgagctggg tcgaatcagc tagcggccgt tgtcctttcg
     2521 cggtggcgac ggaacaggcc gccacgggta cggcgtcgcg gttcgtcggc ttctgtcgca
     2581 ggttcggcca ccggctccgt ccgctccgtc cgctccgtcg tcgcctcggg cacgggcgca
     2641 ggtgcgggct ccgttgcggc gacgagccgc tgcgcgtact gcacgtcgcg caatgtgcga
     2701 acggacaagc ggcccaacac cagtgcgccg aggaatacga tgaccgtccc gagccccgaa
     2761 aagtatgtca ggtccagcag cgcgaacttc gcatcagttg aggccaacgg acgaccgacg
     2821 tcaccgattc tcgccgcctc ggcgactgcg cggccgacca cgaaccaggc ccccgcaaga
     2881 accgtgatcc agccgccgaa catcgcggtg gcgcggttcc gggaggtcag cagcagcagg
     2941 ccaccgagca cggtcgccgc gcccggtagc acttccagcc agccgcgccc cgcggtccac
     3001 atccactctt gattgctgcc ccacgcgaag tcgaagtagg ggccgacgaa tggcaccagc
     3061 gcgccccaca cacccaacag aatgagcaga aatccgcttg ccgcgccgcg gctgcgcgga
     3121 atccgcatcc gtgaaccgcg caccggagca ccagtatcga cctcgttcat gacgcctcct
     3181 taggactacc ccggggtacc cgtcggcgac gacgcctaac cagttcacct aacggcaacc
     3241 aagtgcccgt accgtcgcgc gggtgagcgc gtcgatcagg cactccggcc ccgttgaaca
     3301 gctggtgcgc ttgcgcgccc agatcgacga cctgctgtgc cggctctcgg aagccgaagc
     3361 gacgtggcag gcgtggatcg ccggaacagc tcctgaaaac cgtttcagtg caggcaatct
     3421 cgttcactac tgggcgcttc ggcaggtcga cctgcggcag atgcaatcgg agctcgcatc
     3481 cgtcgggctg tcgtcactgg ggcgcagcga gccgcacgta caggcgtcgc tgcgattggt
     3541 cgcctccgcg atcgctgcga tgctcggcag tggctggtgg ccgcgtgaag acgttgccgg
     3601 acgcgtcgac gagggtgcga agctgctgcg gcagaacgcc gtcgacctgc ttggacaact
     3661 cccagccggg cgcgcagcgc gcatcatggt cacgttgccg tccgaagccg caacggactc
     3721 gtcgctggtg tgcgggctgg tcgaggccgg aatgaacgtc gcgcggatca actgcgcgca
     3781 cgacgacccg cgggcgtggc gagcgatggc cgacaacgtt cgcgcggcgg cggcgacctc
     3841 tgacacctcg tgcctcatcg cgatggatct cggcggcccc aagctgcgca cgggccctct
     3901 ggaggcggga ccttgtgtcg tccggctgca accgcggcgc gacgcgtggg gacaggtcct
     3961 ggctccggcc cgcggctgga tcacctcggc gcagaatccg gtggagccgc cggaggcggg
     4021 gacggtgacc ctgcccgtgc ccgacgactg gctcacccga cggcgaaccg gcgacgtcat
     4081 cacgctcacc gatacgcgcg gatccctgcg gcggttggag ataacaagcg ccccaggcgg
     4141 attcgtcgtc acctgcaata ggacgacgta tctggcgacc ggcaccaagc tcggcatcaa
     4201 gggcgtcgac gatgtcaccg aagtgggcga actggccccg accgaacaaa gcatccgatt
     4261 gcgcgccggc gacattttgc acctcacccg cgactgctcg ccggcaccgg ttgatgcgcg
     4321 ggcaatcccg cgcatcggct gcacattgcc agaggtgttc gaccacgcgc aggtcgggga
     4381 ggccgtgttc ttcgacgacg gcaagctgca cggtcggatc atctccgtcg acccgggtac
     4441 gctggccgtc cgcatcgacc atccgccgca ggggcgcgcg aaactcaagg ccggcaaggg
     4501 aatcaacttg cccgacacaa ctcttcccat ttcggcactg acccggaaag atctagacga
     4561 cttggaaacc gtggtcgaga tcgcggatct ggtgcaagtg tcattcgcac ggtatccgtc
     4621 cgacgtcgag cagcttatcg acgaactggc gcggctcagc gacgaccggc tgggcattgt
     4681 gctgaagatc gagacccggg aggcgttcga acggctgcca cagctattgc tgacagccat
     4741 gaggcggcgc cgcgtcgggg tcatgatcgc ccgcggcgac cttgccgtcg aatgcggtta
     4801 cgagcgaatg gcggaagtgc aggaggagat cctgtggcta tgcgaagccg cgcatctgcc
     4861 ggtgatctgg gctactcaag tactggagca gctcgccaag accggcgtac cctcacgggc
     4921 cgagatcagc gacgccgcaa tgggtgagcg agctgagtgc gtgatgctca acaagggtcc
     4981 gtacatcgac gacgcggtcg tcgcgctcga caacattctg cgccggatga cgagccatca
     5041 ctacaagaag aacgcacttc tgcgcacgtt gcatgcttgg cagcaggctg ctcattgact
     5101 ccaatgtagg accagcaaca cgatcgcagc caccgggaaa aggccctgca ccagcgccga
     5161 acgcgccttg tccggcgacg acacagccag cacgacggcg gcgagcgcca tcgatccggt
     5221 gcccgcgaag atcagggccg acccgacggc cgtgcagcct gtgccgaaga atgcgatgcc
     5281 caccgcggtg atcaccgcga ggaacaggtt gtagaaaccc tggttcaacg cgagcagttt
     5341 actggtctgc gcttcctcct cggacgtccc gaacaccgct cgggtgcgcg gcgtagtcca
     5401 caggaacgat tccagcaccc agatgtacac gtgcaccagc gccgcgagcg cggcgaatac
     5461 cagagctgcg actgccatgt caggcctcgg ttccggcgct gtcgtcgacg ctctcgggcg
     5521 tcgcacgcac cccgctcggc gcgacgagca tgaccaaccc gatcaggaag atccacacca
     5581 ggctggacag cgcggccggc aacggtatcc aggtgtgcac aagcggcagc acaccgagca
     5641 cgccgagcgc cgcgctcact ttcgcgccgc cgaatcttat tgtcgcgctg tccaacatcg
     5701 cctcctccct ggaccgggct agtcgaacaa cccggcctga cccaatccgc tgggcggcgg
     5761 gatacccagg tgcctccacg ctagcgccgt cgcgacccgt ccccgcggcg ttcgcgcgat
     5821 catcccggcc cgcaccagga acggctcgca cacctcctcg accgtcgtgg cctcctcgcc
     5881 caccgcgacc gccaacgtcg acaccccgac cggcccgccg ccgaagctgc ggatcagcgc
     5941 cgtcagcacc gcgcggtcca gccggtccag cccgagttcg tcgacgtcgt agaccgccag
     6001 cgcggtcttg gcgacgtcgc gggtgatcac accgtcggcc cgcacttccg cgtagtcgcg
     6061 gacgcgacgc agcagccggt tggcgatgcg cggagtgccc cgcgagcggc gcgcgatttc
     6121 ggcaccggcg tcggcgacca cctcgatgcc cagaatgccc gccgaccggc gcagcacctg
     6181 ctcgagttcg gccggctcgt agaagtccat gtgcgcggtg aacccgaacc ggtcgcgcag
     6241 cggaccggtg agtgcgccgg accgggtggt ggcgccgacg agcgtgaatg gcgcgacttc
     6301 gagcggaatc gacgtcgccc caggcccttt gccgacgacg acgtcgaccc ggaagtcctc
     6361 catcgcgagg tatagcatct cctcggcggg ccgggcgatg cggtggatct cgtcgatgaa
     6421 caacacgtcg tgctcgacca ggttcgacag catcgcggcc aggtcgccgg cacgctccag
     6481 cgcgggaccc gacgtcagcc gcaacgagct acccagctcg tgggcgatga tcatcgccag
     6541 cgacgtcttg ccgagtcccg gcggaccgga cagcaggatg tgatccggtg tgccgccgcg
     6601 gttcttggcg ccctcgatga ccagttgcag ctgctcgcgc acgcggggtt ggccgatgaa
     6661 ctcgtccagc gaccgcggcc gcaggctggc gtcgacgtcg ctctcgccga cggtgagcgc
     6721 cgcggacacc tcgcgctcgt cggcctcgtc gatcggctcg aaccgggtca tttcctaccc
     6781 aacagcgaca gcgcggcccg cagcgcgctc gacgtggtgg cctcgccgtt ttcaccggac
     6841 agcacccggt cggtggcctc ctcggcttgc ttggccgcaa agccaaggcc cacaagggct
     6901 tccaccaccg gtccgcgcac cgcgtggccg ttggtggcgg gcgcaccgga tgtggacggc
     6961 cgtgcgggtc ccactttgtc gcgcagttcc agcaccatgc gttcggcgct gcgcttgccg
     7021 atgccgggca cgcgggtgag cgcggtgacg tccccgtcgg ccaacgcttg gcgcagcgcg
     7081 ggcgcttcgt gcacggcgag cgtcgccatc gcgagccgcg gaccgacacc ggagacggac
     7141 agcagcgtct ggaacaggtc gcgggtgtcg gaatcggcga agccgtacag cgtcatcgag
     7201 tcctcgcgca cgatcatcgc cgtgacgagc cgggcctcgc tgccgcggcg caaggtcgca
     7261 agcgtcgacg gcgtcgcgtt cacccggtag ccgaccccgc acgcttcgat caccacgtgg
     7321 tcgagggcca cgtcgaggac ttccccacgg atggacgcga tcatctcgcc gccttcactt
     7381 tcgcttcgta cttcttgcgc tgctcggcgg acagcgcttc ggcctgcgcc atccgcgcca
     7441 gcataggcgc acgccagcag tgacagatcg ccagcgccaa cgcgtcggcg gcgtcggccg
     7501 gtgccggttt ggtctgcagc gcaaggattt tggtgaccat cgtggtgact tgcgacttgc
     7561 ccgcacctcc ggagccggtg acggcggcct tcacctcgct gggcgtgtgg aagtgcacat
     7621 cgatgccgcg gcgggcggcg gcaagggcga tcactccccc ggcctgcgcg gtgcccatca
     7681 ctgtcgacac gttctgctgg gaaaacaccc gctcgatcgc gatcacgtcc ggctgatgcg
     7741 tgtccatcca gtgctcggcg acgtcgctga tcgccagcag ccgccggtgc aggggcatgt
     7801 cggtgggagt gcgcaccacg tcgacgtcga gggcggtcac gtgccggccg ccggagctct
     7861 cgatcagcga cagcccgcag cgcgtcagcc ccgggtcgac tcccatgaca cgcactgacg
     7921 cctcctgccg aacaattgtt cgacagaacc ttaacgggtt gacccgacac taccgcgcaa
     7981 cgacacgcgg cgctttactg cggcaccggc gccgggtact gctgggccag tgcgccggct
     8041 tccgcagcct gtgcgagctc gtcgacacgg ttcccggacg cgcgccaacc gcacaccagt
     8101 tcctgcccga cctttttccc gtcgcgcgat cgggagatgg gtagcgcggc ggacccccgg
     8161 ctttagccgt tggggtgagc gcgtcaagtt catgcctgtg gtgaccgctg cccgtgcgtt
     8221 ccggcgcgtt gagtgtgtta gatagtgcct tgctgtctac tgttgtcggt cgtgggtgtt
     8281 acgttgcgga cgaacgcgaa tatcgcgttc cagtgcgcgt ttcacgtggt gtggtgttcg
     8341 aagtaccgcc gagctgtgat cggcgggcgg atggaacaac ggctcaagca gatcatcgcc
     8401 gaggttgtcg aggagaaggg ggcatggttg gtggagctgg aaaccatgcc cgatcacgtg
     8461 cacctgctgg tggaggtgga tccgcagttc ggggtgcaca aattggtgaa ggccatcaag
     8521 ggccgctcgt cgcgggcgtt gcgggaggag ttcccgtggc tgcggtcacg gttgccgtcg
     8581 ctgtggacga actcctactt cgttgcgacg gtgggcaggg cgccgttgtc ggtgatcaaa
     8641 cgctacgtcg agactcagaa gagccggtga gtgggttgtg ttgacggggc ggcggtttcg
     8701 ggtggagttc accgccgagc aggccgagta cgctgaccgg atcgcttcgg tgtgccgggc
     8761 ggtgtggaac accggcttgg agcagcgccg cgagtaccgg cgccgcagcg gatggatgaa
     8821 ctaccgcgag caggccggcg agctggccga ggcgaaggcg gagcaccggt ggctggccga
     8881 ggtgcccggg cattgtttac agcagacgct gatggatctg gacaaggcgt gccgggcgca
     8941 tggcacgttc cgggtgcggt ggcgctccgg ccggcggtgg cggccgtcgt tccggtttcc
     9001 cgaaggtaac aagatggttg tcaagcagct caacggtcgc catgcgcggg tgaagctacc
     9061 caagctgggc tgggtgcggt tccgtttgtc gcggccacac gacgatgttg tgctgcgctc
     9121 ggcaactctg actcgtgacg gccgtcattg gttcgtgtcc ttcctcgtag acgacggact
     9181 ccagaccccc gaggtgcaca ctgcgccgac agcggcggta ggtgtggatc gcggcgtggt
     9241 gacagcgctg gcgacgagcg acggtgagct gttcgaccgg cagttcctga ccgccggcga
     9301 gcaggtccgg gtggtaaggc tgcagcgcaa gctctcccgt gcggcgcggc ggggccgtaa
     9361 cagagacaaa gtccggtcgg cgctagccga gatccgggag cgggaggcgc gtcggcggct
     9421 ggacttttgc gcccaaactg cccaccggct cgcggccgcg aacgcggtag tcgtgctcga
     9481 aaacctgaaa accaagcaga tgacccgttc agcgaaagga accctcgaac agcccggaac
     9541 taacgtcaga gcgaagtccg ggctcaaccg cgccatcctc gccaaggggt ggcaccagtt
     9601 cgccctggcg ctgtcctcgg cggcccgcta cagcgggacg cgtgtggtgg cggtgccggc
     9661 tgcgttcacg tcgcaacgct gctcagcttg tgggcatctg gacccgaaat cccgtgagag
     9721 ccaagcggta ttccggtgca ccagctgcca gcacgggccg gtgcacgcgg acgtcaacgc
     9781 tgcgaagaac ctactggcca tagggcttgc ggccacagcc tgctaagacc acgcgcgccc
     9841 cttgggacgt gcgcggtcgt tgaaacagga accagcagga aaccgcgagg aattactgct
     9901 ccaacccacc taacagaggg gttggaatcc cccggcttca gccgtgggga ggacgtcaag
     9961 aggaaaatta tcatgtcgag gaagccgatg ccggtcgtac gggaccgtct tgaaccacca
    10021 ccggtgcacg gaccacgtca ccccgttggg tctgtcgcct ctgccacccc acggacccct
    10081 attcgatacc ggaatgtgga gagtatgttt ccgacatgac ggccggcggc gtgcggacgg
    10141 tccggagttt ctgccgggtc tgcacgtcgg tgtgcgggat cctcgtcgac gtctcgggtg
    10201 acgaggtgat tcgggtccgc ggtgaccgcg agcatccgct gtcgcacggc tacacctgcc
    10261 cgaaaggccg tgcgctgccg cagatgcacc atcaccccga gcggttcgaa cgcccgctca
    10321 tcagaaccga cggcggccta caaccgacaa cctgggacgc ctgcctcgac gacctctgcg
    10381 tcaagctgcg cgacatcatc gaccgccacg gccccgaagc cgtgggcatc ttgttcggca
    10441 ccggggtcgg tatggacgcc gccggatacc gaatggcgca gggccttcac gccgcgatcg
    10501 gcacaccggc gaagttcagt ccgctgacca tcgacggcac cgccaaggtg ttgatctccg
    10561 agctgatggg cggttcgtcg gcactcaccg gtcgccccga ctacgacggt gccacgctcg
    10621 tcatgttgat cggcagcaat cccgttgtgt cgcatggtca caccgtgggg atgcccaatc
    10681 ccaccggggc gatccgcgac ttgacgaagc gcgcacaggt gtgggtcgtc gacccccgcc
    10741 acaccgagac cgcgcggctg gccacgcggc acctcgcgcc gcgtccgggc accgactacg
    10801 cggtgctggc cttcttggtg cgagaggtgc tgcgcgacgg tgtcgaggtg ctcgtgcagg
    10861 gtgtcgacga gctcgccgcg gcggtcgaac cgttcacact cgcgcacgcc gccgaggtcg
    10921 ccgacgtttc gcgggcggac ctgtcggagc tgctggcggc ggttcggcgg gccgggcgcg
    10981 tcgcgatcga caccggcacc ggcgtgacga tgtcggcgag cgccaatgtg acgcaatggc
    11041 tttcgtgggc attgctgatc ctgaccgact cgatgaaccg gcccggtggc tactggtttc
    11101 accccgggtt cgcataccag ctcgaagcct tcgagctgcc gatctcgcca ccggagggca
    11161 cgttcggccc ggggccgcgc agccggcccg aaacccaagc attcctgcgc gagtggccgt
    11221 gcgcggtgct gcccgacgag atcatggcag gcaacatccg ggcgtttctg aatctcggcg
    11281 gcagcctcgt cacggctttc cccgacgccg ggtcgctggt gccggcgctg aagcaactcg
    11341 atgtctttgc gacgacggag atcctgccga acgagaccac cgcgctgtcg acgcacgtgt
    11401 tgcccaccaa ggaccagctc gagcggcccg acgtgacgct ctgggacatc ctcagtccgc
    11461 gcgtctgcgc gcagcacacc tcggcggtcg tcgacgcggt cggcgagcgg cgctcgatgt
    11521 ggtgggtgct cgcggagatc ggccgtcgac tcggacacca gctggccgac accagcctgt
    11581 ccgacgacgc gatgctggcg agcctgtccg gcggtggccg gtgtagtttc gaggagctgg
    11641 ccgcctccgg ctatgtggag acgccgcgcg agctgccggc gccatgggta gagcggcaca
    11701 tcgggcggtt gggcggctgg cggctggcgc cgccgttgct ggtcggtcag ctggcggcgc
    11761 tgcgggaacc tgccccgctg atgctggtgc cgcgccggca gaggcggcgg ctgaactcgc
    11821 aactggagtt tctgggtgag agcgccgagg tgctgctcca tccggatgac gccagggctg
    11881 ccggggtcgt cgacggtgag cacgtgatcg tgcgaagcca gcacggcgag ctgaccggga
    11941 tcgcgaaggt ggagccgtcg ataaggcggg gtgcggtgtc ggtgccgcac ggccacgctg
    12001 aggccaacgt caacctgctc acgtcgaagg acgacatcga cctggtgacc ggcatgacgc
//
LOCUS       DASWBF010000142         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_537175,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000142 DASWBF010000000
VERSION     DASWBF010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537175"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VF874_04810"
     CDS             complement(<1..563)
                     /locus_tag="VF874_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9175435.1"
                     /translation="MPCRAVRPRVALAFEADSQRLDLARHCRVLPLDRVEGHGAPIAD
                     DSDTVLDPDVPAVVVFTSGTTSAPKGVIHTLNSLRCATAKMVAALEVTSDDAFFLSSP
                     LASITGVLQLESALAVHGKLILEDRFSAGSSLDRVCRYGATIIGGAPVIAESLFAEAD
                     RRGLHALPLRCVAVGGAMVPQTVADTA"
     gene            complement(886..1286)
                     /locus_tag="VF874_04815"
                     /pseudo
     CDS             complement(886..1286)
                     /locus_tag="VF874_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855373.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     gene            complement(1311..1748)
                     /locus_tag="VF874_04820"
     CDS             complement(1311..1748)
                     /locus_tag="VF874_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9175436.1"
                     /translation="MDVQRISDELEISALLSRYARAVDTKDWALYRSVFTDDAYVDYS
                     SAGVIAGSRDEVADWLSQAFTAIAMSMHYITNIEITNVESQVDGDTATVRAMFYNPFQ
                     LSGMSDVSHCGGYYHHELVRGPDGWRSRSLREENLWFENAPTS"
     gene            complement(1748..3232)
                     /locus_tag="VF874_04825"
     CDS             complement(1748..3232)
                     /locus_tag="VF874_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9175437.1"
                     /translation="MTGVQSDTGVLAGEERMLIDGELQHTASGALFDVEHPASEEVVG
                     QAADGTVEDMERAVGAARRAFDTTDWSRDVEFRYHCLKQLQGALEQNKERLRRIVVTE
                     VGCPVSVTGSQIESPIAEVGHWAEHGRGFDYLQDTGMHETQLGPARRKIQYDAAGVVG
                     AITPWNVPFYLNIAETVPPLMAGNTVVLKPAQLTPWSGSELGRIVAEETDIPAGVFNV
                     VVSNANEVGAALSADPRVDMITFTGSTATGRAILAAAAPTVKKALMELGGKSAHIVLD
                     DADLGACLPMAAMMACVMSGQSCILPSRILLPRSRYAEGLEILKASMEAFPVGDPWDP
                     GKLQGPQISETQRQKVLGLIKSGIDSGATLVTGGGIPADLPTGYYTQPTLLVDVDPNS
                     RVAQEEIFGPVLTVTPYDTDDEGIAIANNSIYGLSGEVSSADTERALAVAMRMRTGNV
                     TINGKSHFGITSPFGGTKQSGLGRRNGAEGFLEYLESKTIGMPA"
     gene            3306..3524
                     /locus_tag="VF874_04830"
                     /pseudo
     CDS             3306..3524
                     /locus_tag="VF874_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
BASE COUNT          595 a         1188 c         1203 g          667 t
ORIGIN      
        1 atcgcggtat cggccaccgt ctgcgggacc atcgcgccgc cgaccgcaac gcaccgcagc
       61 ggcagagcat gaaggccccg ccggtcagct tcggcgaaca gcgattccgc gatgaccggg
      121 gctccgccga tgatcgtcgc accgtatcgg cagacccggt caagcgacga gccggccgaa
      181 aaccggtcct ccagaatgag tttcccgtgc accgccaacg ccgactcgag ctgcagcacg
      241 ccggtgatac tcgccagcgg gctggacaag aagaacgcgt cgtcgctggt gacctcgagc
      301 gcggcgacca ttttcgccgt ggcgcaccgg aggctgttca acgtatggat gacacccttc
      361 ggcgcgctcg tcgttccgga ggtgaagacc acgacggcgg gcacgtcggg atcgaggacg
      421 gtgtcgctgt cgtcggcaat cggtgcgcca tggccctcga cgcgatcgag tggcagcact
      481 cgacaatgcc gggccaggtc gagccgctgc gagtcggctt cgaaggccag ggcgacccgc
      541 ggccgcaccg cacggcacgg cacgcgttga ggagatcgga ttcaccgcat ctgcgatcca
      601 acagcaccac gaccgcaccc aggtccagga ctgcgaggta ggcggccacc gcagcgttgc
      661 ggagcggggc gagcaccagc accgcgtcat cggcagtcac cgcagcattc gtgagttcag
      721 cccggacgcg ggacaccgcg gcggccaact gtttatgggt ccactgagac tcattgtcta
      781 ccagcagtat tcgctggcca cgccccgatc tggaaggttg ccagtatcct gcgcggcggt
      841 accgagtcgc agcgtccgcc ggcgggggag aggtggcgcg tattctcacg acaccggcag
      901 cgactccaaa tgctgcgcga tcgcagcgtg atactcgtcg atgcgcgccg gattcacgat
      961 ccggaagaac ccatccggca tcggcacgtc cttgtgcgcc tcgatggtca tcgtccgggt
     1021 gaagagcgtc atgttctcgc cgggccgggt gaagtggtca gcctcggtcg catcgcgacg
     1081 gccaactgcc aggtgaaggt gacgtccttt tcggggtgcg actcgtccca cgtgtcgccg
     1141 accttcagcg ggagttccgg cacgttgctg attcgagggc tgccggggta ggccctcgtc
     1201 cagttggtcg ggttggtgac gaagtcgtag atcatctcgg gaggttgcgt gaatgcggtt
     1261 tccgagcttg tggtgacgat gcccaatgac gttgcctctc tgtgtggtac tcagctggta
     1321 ggcgcgttct cgaaccacaa gttctcttcc cgcaggctgc ggctacgcca tccatcgggg
     1381 cctcgcacca gctcgtggtg gtaataaccg ccgcagtggc tgacgtcgga catgccagaa
     1441 agctgaaacg ggttgtagaa catcgcccgg acggtggcgg tgtcgccgtc gacctggctt
     1501 tcgacgttgg tgatttcgat gttggtgatg tagtgcatgc tcatcgcgat cgcggtgaat
     1561 gcctggctga gccagtcggc gacctcgtcg cggctgccgg cgatgacgcc ggccgacgag
     1621 tagtcgacgt aggcgtcgtc ggtgaacacc gatcggtaca gcgcccagtc cttggtgtcc
     1681 acggcgcggg cgtatctgct gagcagcgcg gagatttcga gttcgtcgct gattcgctgg
     1741 acgtccatca ggccggcatc ccgatcgtct tactctccag atactcgagg aagccctccg
     1801 caccgttgcg ccggccgagc ccgctctgct tggtgccgcc gaacgggctg gtgatgccga
     1861 agtgggactt gccgttgatg gtgacgttgc cggtgcgcat ccgcattgcg acggccaatg
     1921 cccgctcggt atcggcgctg ctgacctcgc cggacaggcc gtagatcgag ttgttggcga
     1981 tcgcgatgcc ctcgtcgtcg gtgtcgtacg gggtgacggt gaggacgggt ccgaagattt
     2041 cctcttgggc gacgcgggaa ttggggtcga cgtcgacgag cagcgtgggt tgggtgtagt
     2101 aaccggtcgg caggtccgcc gggatgccgc cgcccgtgac gagagtggct ccggaatcga
     2161 tcccggattt gatgaggccg agcaccttct gccgctgagt ctcgctgatc tggggcccct
     2221 gcaacttgcc cgggtcccac ggatcgccca ccgggaacgc ctccatgctg gccttgagga
     2281 tttccagtcc ctcggcgtac cgactgcgcg gcaacaggat tcggctgggc aggatgcaac
     2341 tctggccgga catcacgcag gccatcatcg cggccatcgg aagacacgca cccaggtctg
     2401 catcgtcgag cacaatgtgc gcggacttgc cgccaagctc catcagcgcc ttcttcaccg
     2461 tcggcgccgc ggccgccagg atcgcccgcc cggtcgcggt cgacccggtg aacgtgatca
     2521 tgtccacccg agggtcggcc gacagcgcgg cgcccacttc gttggcattg gataccacga
     2581 cgttgaacac gccggcgggg atgtcggtct cctcggcgac gatccgaccg agttcgctgc
     2641 cggaccacgg cgtgagctgg gcgggcttga ggacgacggt gttgccggcc atcaatggcg
     2701 gtacggtctc ggcgatgttc aagtagaacg gcacgttcca cggggtgatc gcgccgacga
     2761 ccccggcagc gtcgtactgg atcttgcgcc gcgcgggacc cagctgcgtt tcgtgcatgc
     2821 cggtgtcctg caggtagtcg aacccgcggc cgtgttcggc ccagtgaccc acctcggcga
     2881 tcgggctttc gatctggctt ccggtgacgc tgaccgggca gccgacctcg gtcaccacga
     2941 tgcggcgtaa ccgctccttg ttctgctcga gggcgccctg caactgcttg aggcagtggt
     3001 agcggaactc gacgtcgcgg gaccagtccg tggtgtcgaa cgcccggcgc gccgcgccga
     3061 ctgcgcgctc catgtcttcg accgtgccgt cggcggcctg cccgaccact tcctcgctcg
     3121 ccgggtgctc gacgtcgaac aacgccccgc tcgcggtgtg ctgcagctcg ccgtcgatga
     3181 gcatccgctc ctcgccggcc agcacgccgg tgtcgctttg gactcctgtc atcgacccgc
     3241 tccttccaac gaccaatcta caatctaagc tttacagatt aaggcgaaac tgtcacgctg
     3301 ttctggtgac cacctcgatc ctggtgttcg acgcgttctc ggaggaattc ttcaacgacc
     3361 ctattgagac ctaccggcgc atgcgcgacg aggcgcccgc gtactacagc gagcgctatg
     3421 acttctacgc gctgagtcgc cacgaagacg tcgcggccgc gttcaaggac ttcgaaacgt
     3481 actcatcgtc ccggggtgtg acgctggagg agatccagtc gggaccacca ggtcagagca
     3541 tgttttcacc acagccgggc gatatcaggg acacctacgc gaccaacacc gggcgcacct
     3601 gtccccgctc ccggtagcgt cgcgcgatct cggcgtcgac gtcgtcggcc agt
//
LOCUS       DASWBF010000143         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_410665,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000143 DASWBF010000000
VERSION     DASWBF010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410665"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..137
                     /locus_tag="VF874_04835"
     tRNA            46..137
                     /locus_tag="VF874_04835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Ala,seq:tgc)
     gene            175..1104
                     /locus_tag="VF874_04840"
     CDS             175..1104
                     /locus_tag="VF874_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9175438.1"
                     /translation="MRVLMFSAGVIGSVYAGRLHDAGHDVTLFARGDRLAALRDHGLV
                     LEDAHTGRRTVRRLPLLPTLNEAISFDAIVVAVRREQFADATAALARSPITGDVVLFG
                     NAAGTTQQFTSMLGPRALIGFPAAGGVGDGEVIRYVLIRQQKTMLAEPGGMITTRLHT
                     LADALKRSGFATTLSTDGDGWLTAHAAFVVPIALALYRVDVDPTRLAADPATIRLMVR
                     ATRQAFLALRGAGNRQVPRNLRALYLYAPEWLAVAYWRRTMASPNGELWFGAHCREAR
                     EEMIGLADVVRDACAVSQHRTPELAELLGSSDR"
     gene            complement(1101..1517)
                     /gene="cwsA"
                     /locus_tag="VF874_04845"
     CDS             complement(1101..1517)
                     /gene="cwsA"
                     /locus_tag="VF874_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthesis protein CwsA"
                     /protein_id="HEX9175439.1"
                     /translation="MTTTSLTPRQRLSRGLQYTAVGPVDITRGTVGLGLHGAYSAGSR
                     LRQRYRESRVASEVGAAQETLARELAAAQEIVANLPQALQDARQPKNRRRRIALVTAV
                     GVAALAGGAVAFSIIRRSMQPEPSPRPPSVEVAPKP"
     gene            1600..2130
                     /locus_tag="VF874_04850"
     CDS             1600..2130
                     /locus_tag="VF874_04850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9175440.1"
                     /translation="MTTSPIQTATATLHTNRGDIKIALFGNHAPKTVENFVGLATGTK
                     DYTTQNASGAPSGPFYDGAVFHRVIDGFMLQGGDPTGTGRGGPGYQFADEFHPELQFD
                     KPYLLAMANAGPGTNGSQFFITVGRTPHLNRKHTIFGEVVDPESQKVVDAIATTDTDA
                     RDRPTEPVVINSITVE"
     gene            complement(2131..2547)
                     /locus_tag="VF874_04855"
     CDS             complement(2131..2547)
                     /locus_tag="VF874_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX9175441.1"
                     /translation="MQQTEWGPNPVAIAGCGLLGIVLSVAAVTLVTDAPGRLLVGAAG
                     VGLLIFAGFSFRARPKLAITSDGLVIRGWWRTTVLPKSAIEHIRMTEFRRLARKVRLL
                     EVDTVDGRLTVFSRWDVGTDPIEVLDALTAAGYAGR"
BASE COUNT          491 a          930 c          899 g          439 t
ORIGIN      
        1 ctggacgccg agcgctccga ctcgcgccgg cgacgcgccc acgacgtcgc agtgacgatc
       61 gtgccgaaca ccagcagcgc ctcgcgcgat ttgcctttgc aaggcagaag tcaggggttc
      121 gaatccccta ggctccacaa ctttgtgtga ccgcctgccc gcggcggatt gcccgtgagg
      181 gttctgatgt tcagcgccgg cgtaatcggc agcgtgtacg cgggccgtct gcatgacgcc
      241 gggcacgacg tcacgctgtt cgcccgcggc gaccggctgg ctgcactgcg cgaccacgga
      301 ttggttctcg aagacgccca taccggacgc cgcacggtcc gtcgactgcc actgctgccc
      361 accctgaacg aggcgatcag cttcgacgcc atcgtcgtcg ccgtgcgccg agagcagttc
      421 gctgacgcaa ccgccgcgct ggcccggtca ccgattacgg gcgacgtggt gttgttcggc
      481 aacgcggccg ggacgacgca gcagttcacg tcgatgctgg gcccgcgcgc cctgatcggg
      541 tttcccgccg ccgggggtgt cggtgacggc gaagtgattc gttatgtgct tatccgtcaa
      601 cagaaaacga tgctggcgga acctggcggt atgatcacca cccgcttgca cacgttggcc
      661 gacgcgctca agcgttcggg gttcgcaacc acgctgagca ccgacgggga cgggtggctg
      721 acggcgcacg ccgcgttcgt cgtaccgatt gcgctggcgc tctaccgcgt cgacgtggac
      781 cccacacgac ttgcggccga cccggcgacg atccggttga tggtgcgcgc gacgcggcag
      841 gcgtttctcg cgctgcgtgg cgccggcaat cgtcaggttc cccgcaatct tcgtgcgctc
      901 tatttgtatg cgccggaatg gttggcggtc gcgtactggc gacgcacgat ggcgtcgccc
      961 aacggcgagt tgtggttcgg cgcgcattgc cgcgaggcgc gtgaagagat gatcggcctg
     1021 gcggacgttg tgcgggacgc gtgcgcggtg agccagcacc gcacacccga gctcgctgaa
     1081 ctgctcggct cgtctgaccg ctagggcttg ggcgccacct ccacactcgg cgggcgcggc
     1141 gacggctcgg gctgcatcga ccggcgaatg atcgagaacg cgaccgcgcc gccggccagg
     1201 gcggctacgc ccaccgccgt caccaatgcg atccggcgcc ggcggttctt gggctgccgg
     1261 gcgtcttgca gcgcctgcgg caggttcgcg acgatctcct gggcggcggc cagctcacgc
     1321 gcgagggtct cctgcgccgc gccgacctca ctggcgaccc ggctctcgcg gtagcgctgg
     1381 cgaagacggc tgcccgccga gtacgcgccg tgcagaccga gaccgacggt gccgcgggtg
     1441 atgtcaacgg gtcccacggc cgtgtactgc aatcctcggc tcagacgctg ccgcggtgtc
     1501 aggcttgtgg ttgtcacggt tcacctttct gccggtgggt tgagcgtatc ggacggtggc
     1561 tgccagaccg gccgaacggg cgtggcacac tgacatgtcg tgaccacgag cccaattcaa
     1621 accgcaaccg ccacgctgca caccaaccgc ggtgatatca agatcgccct gttcggtaac
     1681 cacgccccca agaccgtcga aaatttcgtc ggattagcaa cgggcacaaa ggattacacc
     1741 acgcagaacg catcgggcgc gccgtccggg ccgttttacg acggcgcggt cttccaccgc
     1801 gtgatcgacg ggttcatgct ccagggcggc gacccgaccg gcaccggtcg cggagggccg
     1861 ggctatcagt tcgccgacga gttccatccc gagctgcaat tcgacaagcc gtacctgctg
     1921 gcgatggcca acgcgggacc gggcaccaac ggctcacagt tcttcatcac ggtgggcagg
     1981 acgccgcacc tgaaccgcaa gcacacgatc ttcggcgagg tcgtcgaccc cgaatcacag
     2041 aaggtggtgg acgcgatcgc caccaccgac accgacgccc gcgaccggcc gacggagcca
     2101 gtggtgatca actcgatcac cgtcgaatag tcagcggccg gcgtagcccg cggcggtcag
     2161 tgcgtcgagc acctcgatcg gatcggtgcc cacgtcccac cggctgaaca cggtcagccg
     2221 accgtcgacg gtgtcaactt cgagcaagcg cacttttcgc gcgagccgac ggaattcagt
     2281 catccggatg tgttcgatcg cggacttcgg cagcaccgtg gtacgccacc atccgcggat
     2341 gactagacca tcagacgtga ttgccagctt cggtcgcgcc cgaaacgaga agcccgcaaa
     2401 gatgagcagg ccgaccccgg ctgccccgac cagaagccgg ccaggagcat ctgtgacgag
     2461 cgtcacagcc gcgaccgaca ggacgatccc gaggagtccg caccccgcaa tggcgaccgg
     2521 attcggtccc cactcagttt gctgcacggc ccgcccatgc gccgttgcca caccaaatga
     2581 agttatccac aggtgttatc aacaatgggg atgaatcaca tcggtgtgat tgggtgacgg
     2641 caaagtttcc agcacagaaa cagtgcggaa acatcgggtg cggaacaagc atcgacggcg
     2701 gctcagcgcc agcgcatcgt gagcaacaaa ccggagatca ggaaagcaaa cgcgatccc
//
LOCUS       DASWBF010000144         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_141489,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000144 DASWBF010000000
VERSION     DASWBF010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141489"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VF874_04860"
     CDS             complement(<1..748)
                     /locus_tag="VF874_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX9175442.1"
                     /translation="MTSTDSAPAFHVRLNNFEGPFDLLLQLIFAHRLDVTEVALHQVT
                     DEFIAYTKAIGPQLELDETTAFLVVAATLLDLKAARLLPAGEVEDAEDIALLEVRDLL
                     FARLLQYRAFKHVAEMFAELEAAALRSYPRAVSLEDCFADLLPEVMLGVDATSFAQIA
                     ATALTPRPVPTIAIEHLHETIVSIPEQAGQVLDALQRRGPGQWTTFSELVADCSEAIQ
                     IVGRFLALLELYRLRAVAFDQSEPLGVLQVS"
     gene            complement(745..1608)
                     /locus_tag="VF874_04865"
     CDS             complement(745..1608)
                     /locus_tag="VF874_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9175443.1"
                     /translation="MTDEADHPCVGLTGRLPRTIPEPQPKTTHGPAKVIAMCNQKGGV
                     GKTTSTINLGAGLAEYGRRVLLVDLDPQGALSAGLGVPHYELDATIHNVLVESRVSID
                     DVMVHTKVKHLDLVPSNIDLSAAEIQLVNEVGREQTLGRALYPVLDRYDYVLIDCQPS
                     LGLLTVNALACADGVVIPTECEYFSLRGLALLTDTVDKVHDRLNPKLHISGIVITRFD
                     TRTINAREVMARVVERFGDLVFDTVITRTVRFPETSVAGEPIITWAPKSTGAEAYRSL
                     AREVIDRFGGA"
     gene            1703..>2341
                     /locus_tag="VF874_04870"
     CDS             1703..>2341
                     /locus_tag="VF874_04870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175444.1"
                     /translation="MNLKRRVPLLRWSVWRLALGGRTFMKTGQFGDGREAAAADYVES
                     HARRGDIDDVLTAIDRFAYEKSILINVGDEKGALLDAAVRRADPQLALELGTYCGYSA
                     LRIARAAPAAKVFSVERAAANAEIARRIWAHAGVDDLVTCVVGTIADGGRTLDALATE
                     HGFSDGRLDFVFVDHDKNAYLPDLLSIMACGWLHPGTIVVADNVGMPGSPKYR"
BASE COUNT          395 a          772 c          787 g          387 t
ORIGIN      
        1 acgaaacctg gagcacacca agtggttctg actggtcgaa tgctaccgcc cgcaatcggt
       61 acagctcgag cagcgccagg aaccgcccga cgatctgaat cgcttcggag cagtcagcga
      121 ccagctccga gaacgtcgtc cactggccgg ggccgcgccg ttgcaaagcg tcgagcacct
      181 ggcccgcttg ttcgggaatc gagacgatcg tctcgtgcag gtgctcgatg gcgattgtcg
      241 gcaccggccg cggcgtcagc gccgtcgcgg cgatctgcgc gaagctcgtc gcgtccacgc
      301 ccagcatcac ctcgggcagc aggtcggcga agcagtcttc cagcgacact gcccgggggt
      361 agctgcggag tgcggcggcc tccagctccg cgaacatctc ggcgacgtgc ttgaatgccc
      421 ggtactgcag cagccgcgcg aagagcaggt cgcgcacctc caggagcgcg atgtcctccg
      481 cgtcctccac ctcgccggcc ggcagcagcc gcgccgcttt caggtccaac agcgtcgcgg
      541 ccaccaccaa gaacgcggtc gtctcgtcca attccagttg cgggccaatg gctttcgtat
      601 acgcgatgaa ctcgtcggtg acctgatgca gcgccacttc ggtgacatcg aggcggtgcg
      661 cgaagatcag ctgcaagaga aggtcgaacg gcccctcgaa attgttgagc cggacgtgaa
      721 acgccggcgc tgagtccgtc gacgtcacgc gccgccgaac cggtcgatca cctctcgggc
      781 caacgaccga taagcctccg cgccagtcga tttcggtgcc cacgtgatga tcggttcgcc
      841 ggccacgctt gtctccggga accggaccgt gcgggttatc acggtgtcga acaccagatc
      901 gccgaaccgc tccaccacgc gggccatcac ctcgcgcgcg ttgatggtgc gggtgtcgaa
      961 ccgggtgatc acgattccgc tgatgtgcag cttcggattc agccggtcgt ggaccttgtc
     1021 gacggtgtcg gtcagtagcg ccagcccgcg cagcgaaaag tactcgcact ccgtcggaat
     1081 cacgacaccg tcggcgcagg cgagcgcgtt gacggtcagc agaccgagtg acggctggca
     1141 gtcgatgagc acgtagtcgt agcggtcgag caccggatac agcgcgcggc ccagggtctg
     1201 ctcgcggccc acctcgttga cgagctggat ttcggcggct gacaggtcga tgttgcttgg
     1261 caccagatcg aggtgcttga ccttggtgtg caccatcacg tcgtcgatgg acacccggga
     1321 ctcgacgagg acgttgtgga tcgtcgcgtc aagctcgtag tgcggcacgc cgaggcccgc
     1381 cgacagcgcg ccctgcgggt cgagatcgac cagcagcacc cgccgaccgt attcggcgag
     1441 gcccgcaccg aggttgatgg tcgacgtggt cttgccgacg ccacccttct ggttgcacat
     1501 cgcgatgact ttggccggac cgtgagtggt cttcggctgg ggctcgggaa tcgtccgcgg
     1561 cagccgacct gtcaggccga cgcaggggtg gtcggcttcg tcggtcacgc cggacattcg
     1621 ggcgtagtgc tcgcggacat ccgggcaagc ataactacgg tgaccggttc gttacgggca
     1681 gacgcgcagc tagatttttc ccatgaatct gaagcgacgt gtgccgttgc tgcgctggtc
     1741 ggtgtggcga ctggcgctgg gcggccgcac gttcatgaag accggccagt tcggcgacgg
     1801 ccgagaggcc gcggccgccg attacgtgga gagccatgcg cgccgcggag acatcgacga
     1861 cgtgctgacc gcgatcgaca ggttcgccta cgagaagtcg atcctgatca acgtcggcga
     1921 cgagaagggc gccctgctcg acgccgccgt ccggcgagcc gatccgcagc tcgcgctgga
     1981 gctcggcacg tactgcgggt acagcgcgct gcggatcgcg cgggccgcac cggcggcgaa
     2041 ggtgttctcc gtcgagcgcg cggcggccaa cgccgagatc gcacggcgga tctgggcaca
     2101 cgcgggtgtc gacgacctcg tcacctgcgt ggtcggcacg atagccgacg gcggcaggac
     2161 gctcgatgcg ctggccaccg agcacggctt cagtgacggc cgcctcgact tcgtgttcgt
     2221 cgaccacgac aagaacgcgt acctgcccga cctgctcagt atcatggcgt gcggctggct
     2281 gcatcccggt acgatcgtcg tcgccgacaa cgtcggcatg cccggctcgc cgaagtaccg
     2341 c
//
LOCUS       DASWBF010000145        14604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1262060,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000145 DASWBF010000000
VERSION     DASWBF010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14604
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262060"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1371)
                     /locus_tag="VF874_04875"
     CDS             complement(232..1371)
                     /locus_tag="VF874_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX9175445.1"
                     /translation="MRDVLDELMSIWRSGGTAGVATVVRTLRSAPRSPGAVMVVAPDG
                     SVTGSVSGGCVEAAVYELANEVVRTGRPMLQRYGISTDDAFAVGLTCGGEIDIFAEPV
                     SQALFPQLQEVADAIAAHRPIAVATVIAHPDSRWLGRRLVIGPDDTIGSLGSSRADAA
                     VTDDARGLLSAGRSAVLTYGPNGQRQDAGMEVFVASHAPRPRMLIFGAIDFAAALARQ
                     SAFLGYRVTVCDARSVFATRARFPAADEVVVDWPDRYLAAQAQQGAVDGRTAICVLTH
                     DPKFDVPVLQVALRLPEVGYIGAMGSRQTHDDRLNRLRDVGLSEEEVSRLASPIGLDL
                     GARTPEETAVSIAAEIIARRWRGGGRPLSDLGGRIHHDVSEGAHA"
     gene            1451..2005
                     /locus_tag="VF874_04880"
     CDS             1451..2005
                     /locus_tag="VF874_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX9175446.1"
                     /translation="MTSARAPVVAGVLLAAGAGRRYGKPKVLVDGWLTAAVTSLRDGG
                     CDHIVLVLGAAVVAAPAGVMAVTAPQWQTGPSASVRAGLEQAARLRADYAVLHVIDTP
                     DVGAAVVDRVIQCALASRSGLARAVFGDRPGHPVVIAKQHWPEVLDNLSGDQGAGGYL
                     RSARDVENVDCADLASGRDHDEPT"
     gene            complement(2137..3243)
                     /locus_tag="VF874_04885"
     CDS             complement(2137..3243)
                     /locus_tag="VF874_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9175447.1"
                     /translation="MSEPAGGFAQLKEQSQALDRVRQGLQSWMRTRLEDPKLTVGSLR
                     PPGGTGVANETLLFDVQNSTSEVDGYVVRLSTPDSLYLDYDLTMHYRMYDAMMASPTV
                     PSPAVVGYESDPDVLGAPFFVMEKIDGSIPTDNPSWANEGFVVEASPAQRRALWEGTV
                     RVMAELHRLTPEPFLFLRTGATDDGVGDCLEYWMRSLRWAKPSEPVPLTDEAEQWLLA
                     NLPAGTALSWGDSRLPNVIYRDYTPVGLLDWDLASLAGPQADLAWWIIMTPPKSLQLK
                     GIGTHDEFIDLWQELTGLRATDLHWFLVLGAYRLAAIFAKLFSMYVAQGRLSADVACD
                     QLSGGLHVQLLAGLLDLEPPAGVEPTVPSVRLDR"
     gene            complement(3246..4319)
                     /locus_tag="VF874_04890"
     CDS             complement(3246..4319)
                     /locus_tag="VF874_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX9175448.1"
                     /translation="MSEAVRPAIDMSSGLPDERDFIVPECPDVPMWSENLLFTLYDPD
                     ADVAMWLHLGTVPGKWTMWEDRALVMLPGNEGVLSLRAYHHTAPERRPAGPGLEFRQI
                     EPWRRWHVSFDGFGLYTSESEMLSGVSRDGPGKPLSADLDIELLTPAWDARTAASLES
                     GAGDMDSHDWASEHYEQLVRASGTVTLPLDETVPFCGYGWRDHSRGARDHSTLLDWGG
                     HVILGCPYPSGKGWGLSRYYASDGRITLEGGYVFVDGRFEHARVQTAPRLRELCFGGE
                     VLPVALEWSGGVLNLELHCDRTLWTSMQRSLAVGKDLDGVGLMFVISHGRCDWDGETG
                     YFYSERSDRLNQLAPEPYYAEGS"
     gene            4448..5200
                     /locus_tag="VF874_04895"
     CDS             4448..5200
                     /locus_tag="VF874_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175449.1"
                     /translation="MFALNDKVAVVTGGANGIGAGIAAVLAEAGATVVIADRDRDGAE
                     RQTATLSGAGYRAVVTELDVADEDSIVAVCADIASGVGVPWLLVNNAGLEDRELLLDA
                     TASEWDRINAVNARGAFLMTREVARTMVAAGRGGRIVNIASNVLRGGIVKGLTSYASS
                     KGALVGLSTASAFELAEHGITVNTILPAGVITPGAIGAKGPATEGPATRPTPFGFQDP
                     REIGAAVLFFASPAARPVTNQVLAVDGGFSIA"
     gene            complement(5270..5652)
                     /locus_tag="VF874_04900"
                     /pseudo
     CDS             complement(5270..5652)
                     /locus_tag="VF874_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
     gene            5776..6993
                     /locus_tag="VF874_04905"
     CDS             5776..6993
                     /locus_tag="VF874_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX9175450.1"
                     /translation="MTGRDPHERFRVATPLELLFDLTFVVAFGVAASEFAHALAENHV
                     GSGLLAFVFATFAVWWAWINFSWFASAYDTDDWMYRLATMVQMVGVVILALGLPQTFA
                     SVVKGHYIDNRIMVAGYVVMRIAMVAQWLRAAKQDPVHRPACLTYAVNVTIAQIGWVG
                     LFIAHTTFGVTLVFVCVLIGVELLGPFIAETRKGGTPWHAHHIAERHGLLTIIALGEG
                     VIGTVASVSAVVSAQGWSVDAALVALAGIGLTFGMWWTYYVIPAAPLLHVHRQRSFTF
                     GYLHFPIILGIVATGAGLHAAAYYVEHHSKLGSAATVLTVAVPVAAYLATVYLLYMLL
                     VHTRDAFHVLLVVLTAVVLVAAVGLAAAGLSMPVCLLIVTLAPVVTVVGFELLGHRRA
                     TEAVNRSLADGTT"
     gene            complement(6999..8357)
                     /locus_tag="VF874_04910"
     CDS             complement(6999..8357)
                     /locus_tag="VF874_04910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9175451.1"
                     /translation="MPLADGQSFAGYTILRHLGSGGMGEIYLAQHPRLPRNDALKVLP
                     TTFTGDEEYRQRFNREADIAATLWHPHIVGVHDRGEFEGQLWISMDYVEGTDAARLLQ
                     ESYPNGMPPDEVVRIITAVAEALDYAHQRQLLHRDVKPANILVSHGDSGTERTLLADF
                     GIARWVDDASGLTGTNMTVGTVAYAAPEQLMGEQLDGRADQYALAATAYHLLTGTPLF
                     QHSNPAVVISQHLSAPPPPIGTRRPALSDLGPVFSKALSKAPEDRYDTCTDFADALAQ
                     GLGTVAAETGATEATTASPIAARRRHRARPQRDAKGPSGRRPAVVVAAVLALLLVGVS
                     AVAFVLFHRDETTSAPPASNSPPIPVVAIGADCALLGAEGQTTNGKRAYCAKNPVATD
                     TSLWAYLKEIPTPSVTPGPTDEVYPADIESQVRVCVEETNHTRFQCREDIRRSPNVYG
                     NQ"
     gene            complement(8423..9604)
                     /gene="otsB"
                     /locus_tag="VF874_04915"
     CDS             complement(8423..9604)
                     /gene="otsB"
                     /locus_tag="VF874_04915"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX9175452.1"
                     /translation="MTTPKVTIDPRLHDAVIFVVPQDDSVAELVQRLHGAGVGTATAE
                     GAADPADLRDAAQRMGARAERSVVVDATADGIVAARDGGFSLVIGLGHASELAERGAD
                     VVVDDLAAVGVRTGDSRMSEIPDALESYREVTDVLAGDRPAVCLDFDGTLSAIVPDPD
                     SARLVDGAAEALARLAERCPVAILSGRDLADIRDRVAVPGLWYAGSHGFESIAPDGSY
                     RQNDDAAAAVPVLERAAADLRETLAHIKGTRVEHKRFAVAVHYRDVAADNVGEVAASI
                     HRVGRREHLRVTNGRKVVELRPDVDWNKGTTLEWIREQIARSAGPATSNEVSQKVLPI
                     YIGDDLTDEDGFDAVKADGIGILVRHTEDGDRRSAARFTLENPDRVREFLDRVADDLP
                     G"
     gene            complement(9623..10633)
                     /locus_tag="VF874_04920"
     CDS             complement(9623..10633)
                     /locus_tag="VF874_04920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9175453.1"
                     /translation="MFLHVQLDGRPDQAGERARELAATGVDGLFTFEGQYDVFLPLAA
                     AAATTDAELMTNVAIALPRSPLHLAHAAYDLQLLSGGRFRLGLGSQIRPHIESRYGST
                     WSRPAARMRETALAVRTILEAWQHNRPLEFHGEFTKHTLMTPVFDPGPNPHGIPPIYL
                     GALGPLMTRAAAEVADGLLVMPFHSVRHFRERTLPAIDEGLRRAGRNRADFDIVPQVI
                     AAIGRTDSQLTAAGNGVRALLAFYGSTPAYLPVLEVEGRAELQPRLNQLSKRADVAAM
                     VDLIDDDLLTTLAVRGTPEECAAEILRRFGDIAQRVCVYFPGYDAPGADIRALAAALH
                     AG"
     gene            10796..11527
                     /locus_tag="VF874_04925"
     CDS             10796..11527
                     /locus_tag="VF874_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEX9175454.1"
                     /translation="MSITRRDLLKLAAVAPAAVGAGGALSTVVSAALAPAASAAPLGV
                     LLDYAAGVISAADIRASGAIGAIRYVSDRRPGGDWMKGKPIQLPEARDLYHQGLKIVS
                     CYQFGKQDTADWLGGQAAGVRHAQRGWQLHTAAGGSYAAPIYASIDDDPTPDQYRALV
                     APFLRGWESVLGHQRVGVYANAPTIEWARQDGLGAYYWQHNWGSKGVVHPAAHLHQVE
                     IDKRSVGGVGVDINHILKPNFGQWD"
     gene            12029..>14604
                     /locus_tag="VF874_04930"
     CDS             12029..>14604
                     /locus_tag="VF874_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid synthase subunit beta
                     domain-containing protein"
                     /protein_id="HEX9175455.1"
                     /translation="MTIHEYDRAPAGRDGASPKPAPSAHALVDRLSAGEPYAVAFGGQ
                     GSGWLENLEELVSSAGIEDELAALVAEAEVMLEPIADELVIVRPIGFEPLRWVRAHAA
                     GEPVASAKHLTSAATSMPGILLTQLAAIRALTRQGMDFAATPPVAHAGHSQGVLAVES
                     LRSRGARDVELMVLIQLIGAACTLVARRRGISASGDRPPMVSVTNADPERIQELLDEF
                     AKDVRTVLPPALSIRNGRRSSVITGSPEQLARFELYCQQIADIEAAERKNKLRGGAVF
                     NPTFEPVAVEVGFHTPRLADGVTMIDRWAEKIGLDAKLAHEMAESILVRPVDWVDEVE
                     KLHDAGAHWILDLGPGDILTRLTAPVIRGLGVGIVPAATRGGQRNLFTVGAVPEVAQP
                     WSSYAPSVVTLPDGAVKLSTKFTRLTGRSPILLAGMTPTTVDAKIVAAAANAGHWAEL
                     AGGGQVTEEIFTKRIEELQTLLEPGRTVQFNSLFLDPYLWKLQIGGKRLVQKARQSGA
                     PIDGVVVSAGIPDLEEAVDLIDELNSVGITHVVFKPGTVEQIRSVIRIAAEVPTKPVI
                     AHIEGGRAGGHHSWEDLDDLLLATYSELRARSNITVCVGGGIGTPEQAAEYLTGTWAA
                     RHNFPLMPIDGILVGTAAMATKEATTSPQVKQMLVATQGTDRWIGAGKAQGGMASSRS
                     QLGADIHEIDNAASRCGRLLDEVAGDADAVAARRDEIIAAMANTAKPYFGDVGEMTYL
                     QWLRRYVELAIGEGNSTADAAAPGSPWLADTWRDRFEQMLKRAEARLHPKDFGPIETL
                     FCDEELLERPDDAIVALQSRYPDAETIQLHPADVPFFVELCKTLGKPVNFVPVIDKDV
                     RR"
BASE COUNT         2377 a         4834 c         4972 g         2421 t
ORIGIN      
        1 gaaggtgccg tggccggtga gcagccggga gtcttctacc cgcgggacgc gcgatcccac
       61 gtatcgggtg gcgacggtcc cggaaggggg agaggccatg cttatcgctc cgttccgtcg
      121 atctacttct gcgcgtaagt acagatgcag cgcctctgac agccgacatc agaagtgtcg
      181 ttatcgtaca tcgagaaggc tatgtgcctt tctcggagag tccggtttcc gtcaggcgtg
      241 cgctccttcc gagacatcat ggtgaatgcg gccgccgagg tcagacagcg gccggccgcc
      301 cccgcgccag cggcgggcga tgatctctgc cgcgatcgac accgccgtct cctccggcgt
      361 gcgcgcgccg aggtcgagcc cgattgggct ggcaagccgg ctcacttcct cctcgctcag
      421 ccccacgtca cgcagccggt tcagtcggtc gtcatgtgtc tgccgggagc ccatcgcgcc
      481 gatgtagccg acctccggca gccgcagcgc gacctgcaac accggtacgt cgaatttcgg
      541 gtcgtgcgtg agcacgcaga tcgcggtgcg gccgtcgaca gcgccttgct gggcctgtgc
      601 ggcgagatat cgatcgggcc agtcgaccac gacttcgtcg gccgcgggaa aacgtgccct
      661 ggtcgcgaac accgacctgg cgtcgcacac ggtcacgcga tacccgagga acgcgctttg
      721 ccgtgcgagg gccgcggcga agtcgatcgc accgaagatc agcatccgcg gacgcggcgc
      781 gtggctggcc acgaacacct ccatcccggc gtcctgccgc tggccgttcg ggccgtaggt
      841 gagcacggcg ctgcggccgg ccgacagcag gccgcgggcg tcgtcggtca ctgcagcgtc
      901 ggcccggctg gaccccagcg acccgatcgt gtcgtctggc ccgatcacca gcctgcggcc
      961 gagccaccgc gaatcgggat gcgcgatcac ggtcgccacc gcgatcgggc ggtgggccgc
     1021 gatggcgtcg gcaacctcct gcagctgcgg gaacaacgcc tgcgacacgg gttcggcgaa
     1081 gatgtcgatc tcaccgccgc aggtgaggcc cacagcgaac gcgtcgtcgg tgctgatgcc
     1141 gtagcgctgc agcatcgggc gcccggtgcg cactacttcg ttggcgagtt cgtagaccgc
     1201 cgcttccacg cagccgcccg acaccgagcc ggtgacggac ccgtcgggcg cgacaaccat
     1261 cacggcgccc ggcgagcggg gggccgaacg aagggtgcgg acgaccgtgg cgacgccggc
     1321 cgtgccgccg gaacgccaga tcgacatcag ctcgtcgagc acgtcacgca cgatgcgagt
     1381 gtaggcgctc gcgagatcac tattctcaaa ggcgacacgc gatttccccg tccggaacga
     1441 ggcacgcgaa atgacttcag ctcgggcgcc cgtcgtcgcc ggagtgctac tggccgccgg
     1501 tgccggacgg cggtacggca agccgaaggt gcttgtcgac gggtggctga cggccgcggt
     1561 gacctcgctg cgcgacggtg gctgtgacca catcgtcctg gttctcggcg ccgcggtggt
     1621 tgcggccccc gccggcgtca tggccgtgac ggcgccgcag tggcagaccg gcccgagcgc
     1681 atccgttcgc gccggcctgg agcaagccgc ccgactgcgc gcggactacg ctgtactgca
     1741 cgtcatcgac acccccgatg tcggcgccgc ggtggtagac cgagttatcc agtgcgcctt
     1801 ggcatctcgc agtgggctgg cccgcgccgt gttcggtgac cggccgggcc accccgtcgt
     1861 catcgccaag cagcactggc cggaagtgct tgacaacctc tccggagacc agggcgcggg
     1921 cggctatctg cgcagcgccc gcgacgtcga gaacgtcgac tgcgccgacc tcgccagcgg
     1981 acgcgaccac gacgaaccga cgtgatgtca agctgcgtat gtcggtcatc gagcagcgcc
     2041 gggtaactga ggtgcgtacc gatcaggcgg tcggcgaact tctgcgccag ttgcgccgag
     2101 acgaacgcac ccgaattaca aacggtcgca gcgtcatcag cggtctagcc ggacgctggg
     2161 gaccgttggc tcaacgcccg cgggaggctc cagatcaagc aacccggcca gcagctggac
     2221 gtgcagcccg ccgctgagct gatcgcacgc cacatcggcc gacaaccggc cctgagcgac
     2281 atacatggag aagagtttgg cgaagatggc cgccaagcgg tacgccccga gaacgaggaa
     2341 ccaatgaaga tcggtggccc gtaggcctgt tagctcctgc cagagatcga tgaattcgtc
     2401 gtgtgtcccg attcccttga gctgcaggct tttcggcggc gtcatgatga tccaccatgc
     2461 gagatcggcc tgcggcccgg cgagcgaggc gaggtcccag tcgagtaagc ccaccggggt
     2521 gtagtcgcgg tagatgacgt tgggcagccg gctgtcgccc cacgacaacg cggtaccggc
     2581 aggcaggttg gccaagagcc actgctcggc ttcgtccgtc agcggcacgg gctctgaagg
     2641 tttcgcccac cgcagcgacc gcatccagta ctcgaggcag tcacccacgc cgtcatcggt
     2701 cgcgccggtc cgaaggaaca ggaacggttc tggagtcagg cggtgcaact ccgccatgac
     2761 gcgcacggtg ccctcccaca gcgcgcgccg ttgagctggc gacgcctcga cgacgaagcc
     2821 ctcgttggcc caggacgggt tgtccgtcgg aatggacccg tcgatcttct ccatgacgaa
     2881 gaacggggcg ccgagcacgt cggggtcgga ctcgtagccg acgacggccg gggacggcac
     2941 cgtaggactt gccatcatcg cgtcgtacat gcggtagtgc atcgtcaggt cgtagtcgag
     3001 gtacagcgag tcgggcgtcg aaagcctgac gacgtagccg tcgacctcgc tcgtcgagtt
     3061 ctgcacatcg aacagcagcg tttcgttggc gacgccggtt ccgccgggcg gcctcaacga
     3121 cccaaccgtc agcttcggat cttccaaccg ggtccgcatc cagctctgca ggccctgtcg
     3181 cacccggtcg agcgcctggc tctgctcctt gagctgtgca aaaccccccg cgggctccga
     3241 catggtcacg aaccttcggc gtaatagggc tcgggcgcca gctggttcaa ccggtccgag
     3301 cgttcgctgt agaagtagcc cgtctccccg tcccagtcac agcgtccatg gctgatcaca
     3361 aacatcaggc ccacgccgtc aagatcctta cccaccgcga gactccgctg catcgacgtc
     3421 cacagtgtgc gatcgcagtg caactcgaga ttcaggacgc cgcccgacca ctccaacgcg
     3481 acgggcagca cctcgccgcc gaagcagagc tccctcaggc gcggcgccgt ctgtactcgc
     3541 gcgtgctcga accggccgtc gacgaagaca tagccgcctt cgagggtgat ccgtccgtcc
     3601 gacgcgtagt agcgcgacag gccccagccc ttgccgctgg ggtacggaca cccgaggatg
     3661 acgtggcctc cccagtcgag cagcgtcgaa tgatctcggg cgccgcgcga gtggtcgcgc
     3721 catccgtagc cgcagaacgg gacggtctcg tccaacggca atgtcactgt gccgcttgcc
     3781 cgtacgagtt gctcgtaatg ttcactcgcc cagtcatggc tgtccatgtc gccggccccc
     3841 gattcgaggc ttgccgccgt gcgggcatcc catgcggggg tcaacagctc gatatcgagg
     3901 tcggccgaga gcggcttacc gggcccgtcg cggctgacgc ctgacagcat ttcgctttcc
     3961 gaggtgtaca ggccgaaccc gtcgaacgac acatgccagc ggcgccacgg ctcgatctgc
     4021 ctgaactcga gtccgggacc ggcgggccgg cgttcgggcg ccgtgtggtg gtacgcccgc
     4081 agggagagga cgccttcgtt gccgggcagc atgacaagcg cgcggtcctc ccacatcgtc
     4141 cacttcccgg gaaccgtgcc gaggtgcagc cacatcgcca cgtcggcatc ggggtcgtag
     4201 agcgtgaaca ggagattctc cgaccacatc ggaacgtccg ggcactccgg cacgatgaag
     4261 tcacgttcgt ccggcagtcc cgaggacatg tcgatcgccg gccgcactgc ctcactcacc
     4321 ggcgtgtgat tccgcggtga acatggcgac aagcggatat gttgagcact cgcacagcct
     4381 agtgaaagcg ttcacccgct gaccgctttc ggagggggat ccgaggacga tgtcggtttg
     4441 ttgtgacatg ttcgcgttga acgacaaggt ggctgtggtg acgggcggtg ccaacggcat
     4501 tggcgcgggg atcgcagcag tcctggcaga agccggagcg accgtcgtga tcgcggatcg
     4561 cgaccgtgat ggcgctgagc gccaaaccgc cacgttatcc ggggcgggct accgggcggt
     4621 ggtgacggaa ttagatgtcg ctgacgagga ttcgatcgtc gcggtgtgcg ccgacatcgc
     4681 gtccggggtg ggtgttccgt ggttgcttgt caacaatgct gggctggagg atcgcgaact
     4741 gctgctggac gcgaccgcct cggaatggga tcggatcaac gcggtgaatg cgcgtggcgc
     4801 attcctgatg acccgggagg tggcacgcac gatggtcgct gccggccggg gcgggcgcat
     4861 cgtcaacata gcctcgaatg tgctgcgcgg cggcatcgtc aaaggtctta cttcgtatgc
     4921 ctcttcgaag ggcgcgctcg tcgggttgag caccgcgagc gcatttgaac tcgccgaaca
     4981 tggcatcacg gtcaatacga tcctgcccgc gggagtcatc actcccggtg ctatcggcgc
     5041 gaaggggccg gcgactgagg ggccggccac gcgtccgaca ccgttcggct tccaggaccc
     5101 gcgcgagatc ggtgctgccg tcttgttttt cgcatcgccg gcggctcgac cggtcactaa
     5161 tcaggtgctc gcagtcgatg gcgggttctc tattgcgtag tggcaccgac ggtctcgaga
     5221 cgccgtttag ggtcaccagc tcgtcgccat tgtctccgga ctcaccggcc tattcgtcga
     5281 aacggtgcgg catgtcgcca gggccccagt cgtggtggaa gcggtgtggc ccgtcgccgt
     5341 cgccgtaaac gatcagcgcg accatgaaga cggtgtagcc gatggccagc agcagcaaca
     5401 gcgcgcccgc aatccacaac gagagttgac cgagtcgccg gacccggtcg gatgcggccc
     5461 gggcgcccgc cgcgtcaccg cgcgcccacg ggggatacac attgaacgca tgcgtgaacg
     5521 ccgagaacga cagcggccag aagaacagca gcgatgcgac cgcccatccg gcatgcacgg
     5581 gtggcgtcgg ctctggggcc gaggacgtcc tgtgggcaac ggcgcagact cggaagcggg
     5641 tgtctcggtc atggtgacct cctcatgtcg gctggttacc accgtagaca ccaggtttgg
     5701 gcagcggcgg tcgataatca cggcgtgacg caagaagacc agagggcacc cgtgcctgat
     5761 caccgccttc gccggatgac cgggcgcgat ccgcacgagc ggtttcgtgt cgcaacaccg
     5821 ttggagctgc tgttcgatct gacattcgtg gtcgcattcg gcgttgccgc ttcggaattc
     5881 gcgcacgcgc tcgcggagaa ccatgtcggt tcggggctgt tggcgttcgt gttcgcgacg
     5941 ttcgcggtgt ggtgggcgtg gatcaacttc agctggttcg cgtccgccta cgacaccgac
     6001 gactggatgt accggctggc cacgatggtg cagatggtcg gcgtcgtcat cttggcgctg
     6061 ggcctgccgc agacattcgc gtcggtggta aaaggccatt acatcgacaa ccggatcatg
     6121 gtcgccgggt atgtggtgat gcgcatcgcg atggtggccc agtggctgcg ggccgccaaa
     6181 caggatccgg ttcatcggcc ggcatgcctg acgtacgccg tcaacgtgac catcgcccag
     6241 atcggctggg tgggtctctt catcgcccac accacgttcg gggtcacgct ggtgttcgtc
     6301 tgcgtgttga tcggggtcga gctgctcgga ccgtttatcg ctgaaacgcg caagggcggg
     6361 acaccctggc acgcccacca catcgcggag cgacacgggc tgctgacgat catcgcgctc
     6421 ggtgagggcg tgatcggcac cgtcgcgtcg gtgtcggcgg tcgtgtccgc gcagggctgg
     6481 agcgtggacg cggcgctggt cgcgctcgcc ggcatcgggc tgacgttcgg gatgtggtgg
     6541 acctactacg tgatcccggc ggcgccgcta ctgcacgtgc acaggcagcg ttcgttcact
     6601 ttcggctacc tgcacttccc gatcatcctc ggcatagtcg ccaccggggc cggactgcac
     6661 gccgccgcgt actacgtcga gcaccactcg aagctgggat cggcggccac cgtgctgacc
     6721 gtcgcggttc ccgtggccgc ctacctcgcg acggtgtacc tgctctacat gctgctcgtg
     6781 cacacgcggg acgccttcca cgtgctgctg gtggtgctca ccgcggtcgt gctggtggcg
     6841 gccgtggggt tggcggcggc ggggctgtcg atgccggtgt gcctgctgat cgtcacgctc
     6901 gcaccggtgg tcaccgtggt cgggttcgaa ctgctcggcc accggcgcgc gacggaggcg
     6961 gtcaaccggt cactggccga cgggacgacc tagtgggcct attggttgcc gtagacattg
     7021 ggtgatcgcc gaatgtcctc acggcactga aagcgcgtat ggttcgtctc ctcgacgcac
     7081 acccggacct gcgactcgat gtctgccggg tacacctcat ccgtgggacc cggagtcacc
     7141 gacggagtcg gtatttcttt gaggtaggcc cacaggctgg tgtcggtggc taccggattc
     7201 ttggcgcaat acgccctctt gccgttggtg gtctggccct ccgctccgag gagcgcgcag
     7261 tcggctccga tggcgactac gggtatcggc ggcgagttcg atgcgggcgg ggccgacgtc
     7321 gtttcgtcgc ggtggaacaa cacgaacgcg accgccgaga cgccgacgag cagcagcgca
     7381 agcaccgccg cgaccacgac ggccggccgc cgccctgacg ggccctttgc gtctcgctgg
     7441 ggccgtgccc ggtgccgccg ccgggccgcg atcggtgacg ccgtggtggc ttcggtggca
     7501 ccggtttcgg cggccaccgt gccgagcccc tgcgcgagcg cgtcggcgaa gtctgtgcac
     7561 gtgtcgtaac ggtcctcggg ggccttggag agcgccttgg agaacaccgg gccgaggtcc
     7621 gagagggcgg gacgccgggt gccgatgggc ggcggtggcg cgctgaggtg ctggctgatg
     7681 acgaccgcag ggttggagtg ctgaaacagt ggcgtgccgg tgagcaggtg atacgcggtg
     7741 gccgccagcg catactggtc ggcgcggccg tccagctgtt cgcccataag ctgctcgggc
     7801 gcggcgtagg ccaccgtgcc gaccgtcatg ttggtgccgg tcagcccgct ggcgtcgtcg
     7861 acccagcgcg cgatcccgaa atcggccagc aaggtcctct cggtgcctga atcaccgtgt
     7921 gaaaccagga tgttcgccgg tttgacgtca cggtgcagca gctggcgctg atgggcgtag
     7981 tccaacgcct cggcgaccgc ggtgatgatc ctgaccacct cgtctggcgg catgccgttg
     8041 gggtagctct cctgcagcag tcgcgcggcg tcggtgccct cgacgtagtc catcgaaatc
     8101 cacagctggc cctcgaactc accgcggtcg tggacaccga cgatgtgcgg gtgccacagc
     8161 gtcgcggcga tgtcggcctc gcggttgaac cgctggcgat actcctcgtc gccagtgaac
     8221 gtggtgggca gcaccttgag cgcgtcgttg cgcggcagtc tggggtgttg ggccagataa
     8281 atctcgccca tccccccgga acccaggtgc cgcaggatcg tgtagcccgc gaaactctgg
     8341 ccatcggcca acggcatggg cgcatcctac aagtctttgg cgcggactcc gcgcgatgct
     8401 gcccgttgcc aggttggcag ggttacccag gcaaatcgtc ggcgacgcgg tcgaggaatt
     8461 cacgcacccg gtccgggttt tcgagcgtga accgcgccgc ggagcggcgg tcgccgtcct
     8521 cggtgtggcg caccagaatg ccgatgccgt cggctttcac ggcgtcgaat ccgtcctcgt
     8581 cggtcaggtc gtcgccgatg tagatcggca gcaccttctg ggagacttca tttgatgtcg
     8641 ccggccctgc ggatcgggcg atttgctcgc ggatccactc cagggtggtg cccttgttcc
     8701 agtcgacgtc ggggcgtagc tcaacgacct tgcggccgtt ggtgactcgc aagtgttcgc
     8761 gccggccgac ccggtgtatg ctcgccgcca cctcgccgac gttgtctgcc gcaacgtcgc
     8821 ggtaatgcac cgcgacggca aaccgcttgt gttccaccct ggttcccttg atgtgcgcga
     8881 gagtctcgcg caggtctgcg gcggcgcgct cgagcaccgg cacggccgcc gcggcgtcgt
     8941 cgttctggcg gtagctgccg tcgggcgcaa tcgactcgaa gccgtggctg cccgcatacc
     9001 acaagccggg tacggccacg cggtcgcgga tgtcggcgag atcgcgcccc gacaggatcg
     9061 cgacgggaca ccgctcggcc agtcgagcca gggcctccgc ggcgccgtcg accaaccgcg
     9121 cggagtcagg gtcggggacg attgccgaca gcgtgccgtc gaagtcgagg cagaccgcgg
     9181 ggcggtcacc ggcgaggacg tcggtcacct cgcgatacga ctcgagcgcg tcgggaattt
     9241 ccgacatccg gctgtcaccg gtgcgcacac cgacggcggc gagatcgtcg accacgacgt
     9301 ccgcgccgcg ctcggccaat tcgctggcat gtcctagccc tatcaccaac gagaacccgc
     9361 cgtctcgggc ggcgacgatt ccgtcggcag tggcgtcgac gaccacggac cgctccgcgc
     9421 gcgcacccat ccgctgcgcg gcgtcgcgca ggtctgccgg atccgccgcg ccttcggcag
     9481 tcgccgtccc aacccccgcg ccatgcaatc gctgcaccag ctcggccacc gaatcatcct
     9541 gtggcacaac gaaaatcacc gcgtcgtgca ggcggggatc gatagtcacc tttggcgtcg
     9601 tcacgccgaa atccttgcac acctatcccg cgtgcaacgc cgcggccagc gcacggatat
     9661 cggcgccggg cgcgtcgtag ccggggaagt acacgcagac ccgctgggcg atgtcgccga
     9721 accggcgcag gatctcggcg gcgcactcct cgggcgtgcc tcgcacggca agcgtggtca
     9781 acaggtcgtc gtcgatcaag tcgaccatcg ccgcgacgtc cgcgcgtttg gacagctggt
     9841 tgagccgggg ttgaagctcg gcgcgtccct cgacttccaa tacgggcagg taggcgggcg
     9901 tcgacccgta gaacgccagc agcgcgcgca ccccgttgcc cgccgccgtc agctgtgagt
     9961 cggtgcgccc gatcgccgcg atcacctgcg gcacgatgtc gaagtcggct cggttccgcc
    10021 ccgcccggcg cagcccctcg tcgatcgcgg ggagggtccg ttcacggaag tggcgaacgg
    10081 agtgaaacgg catgacgagg aggccgtcgg ccacctcagc ggcggctctg gtcatcagcg
    10141 ggcccagcgc accgagatag atcggcggaa tgccgtgcgg gttgggtccc gggtcgaaca
    10201 ccggggtcat cagcgtgtgc ttggtgaact cgccatggaa ttccagcggc cggttgtgct
    10261 gccaggcctc gaggatcgta cgcacggcca gcgccgtctc gcgcatgcgc gcggccggac
    10321 ggctccacgt gctgccgtac cggctttcga tgtgcggccg gatttgcgag ccaagcccga
    10381 ggcgaaaccg tccgcccgac agcagctgca ggtcgtaggc ggcgtgcgcc aggtgtagcg
    10441 gactacgcgg caacgcgatg gccacgttgg tcatcagctc ggcgtcggtg gtggccgcgg
    10501 cggcggccag cggcaggaaa acgtcgtact ggccctcgaa cgtgaacagg ccgtcgaccc
    10561 cggtggcggc cagctcccgc gcgcgctcgc cggcttgatc cggacgcccg tcgagttgca
    10621 catgcagaaa catcggccgc accctaccaa ggggtcgaaa ctgctcacag atcgtcgttt
    10681 tcgacttttc gacgatctca cagcagtgtc gacgcaggag gagtgtgaaa agtgatgcga
    10741 ttgttacgga tggcgttggc gttaagtatg gggcaattgt cgtattctgt ggctcgtgtc
    10801 gattacccgg cgcgatctgc tcaaactcgc ggccgtggct ccggcggccg tcggtgccgg
    10861 cggagctttg agcacggtcg tgtcggcggc gctggcaccg gccgcctcgg cggcacccct
    10921 cggcgtcctg ctcgactacg ccgccggtgt gatcagcgcg gccgacatcc gtgcgtcggg
    10981 cgcgataggc gccatccgct acgtctcgga tcgtcgcccg ggcggggatt ggatgaaagg
    11041 caagccgatc cagctgccgg aagcccgcga cctttaccac caagggctga agatcgtgtc
    11101 gtgctaccag ttcggcaagc aggacaccgc ggactggctg ggagggcagg ccgcaggcgt
    11161 gcgccacgcg cagcgcggct ggcagctgca caccgccgcc ggcgggtcct acgccgcgcc
    11221 gatctacgcg tcgatcgacg acgacccgac tcccgatcag taccgtgcgc tggtagcgcc
    11281 attcctgcgc ggttgggagt cggtgctggg ccatcagcgc gtcggtgtct acgcgaacgc
    11341 cccgaccatc gagtgggcgc gtcaggacgg gctgggcgcg tactactggc agcacaactg
    11401 gggctcgaag ggtgtggtcc acccggccgc gcatctgcac caggtcgaga tcgacaagcg
    11461 cagcgtcggc ggcgtcggag tggacatcaa tcacattctc aagccgaact tcggtcagtg
    11521 ggattagctg caacccggtt cagttagcgc tccagcaaac ttttttcgaa ttcgtcagtg
    11581 aacaattgtc gactgaagac ccgagcaagt gcttgcccga atatgcgaag ctggcgagat
    11641 tcgatggaaa gaccttcata acgatttgat aacaatcatg ctgtgaacag cgctgttata
    11701 gctactcgac cgtaaccacc ggcacgcagg acgtttgtca cgcggccccg agggcccggt
    11761 tagaccggtc gtcatctagg ccggggttac taatgcgtag aactcgtcag taaccattcc
    11821 gccgccgggt gaggccggtt tcttatgcca ctcttattgc agccagcggg gcgagctctg
    11881 ccgtggcgga ccgtcgccgg cacgtcgggg tggcccggca tcgctgggat tcgacgggga
    11941 atcgcaacat acgccaagaa aactccaaga cgacgacaag acgatcacgc aagagacatg
    12001 agaccaaagg gttgaggaga cgcggaacgt gacgatccat gaatacgaca gggcgccagc
    12061 tggccgcgac ggcgcttccc cgaagcccgc gccgagtgct catgccctcg tcgatcgtct
    12121 gagtgccggt gagccgtatg cggtcgcgtt cggtgggcag ggcagtggat ggctggagaa
    12181 cctcgaggag ctggtgtcgt ccgccggcat cgaggatgag cttgccgcac tggtggccga
    12241 ggccgaggtg atgctcgagc cgatcgccga cgaactcgtg atcgtgcgcc cgatcggctt
    12301 cgagccgctg cgctgggtgc gcgcccacgc ggccggcgag ccggtggcca gcgccaagca
    12361 tctgacgtct gccgccacgt cgatgcccgg aattctgctc acccagctgg ccgccattcg
    12421 cgcgctgacg cgtcagggca tggacttcgc cgccaccccg cccgtcgcgc atgccggcca
    12481 ctcccagggg gtgctggccg tggagtcgct acgttcgcgc ggcgcccgcg acgtcgaact
    12541 gatggtgctg atccagctca tcggcgccgc ctgcacgctg gtcgcccggc gacgcggcat
    12601 ttccgcatcg ggcgatcgtc cgccgatggt gtcggtcacc aacgccgatc ccgagcggat
    12661 ccaggagctg ctcgacgagt tcgcaaagga cgtccgcacc gtgctgccgc cggcgctgtc
    12721 gatccgcaac ggccggcgct cgtcggtgat caccggcagc cccgagcagt tggcccgctt
    12781 cgagctgtac tgccagcaga tcgccgacat cgaggccgcc gaacgcaaga acaagctgcg
    12841 cggcggcgcc gtgttcaacc ccaccttcga gccggtggct gtggaggtcg gcttccacac
    12901 cccgcggttg gccgatggcg tgacgatgat cgaccggtgg gccgagaaga tcggcctcga
    12961 cgcgaaactg gcgcacgaga tggccgagtc cattctggtt cgtccggtgg actgggtcga
    13021 cgaagtcgag aagctgcacg acgccggcgc gcactggatc ctcgacctcg ggccgggcga
    13081 catcttgacc cggctcaccg cgcccgtgat ccgcggcctg ggcgtcggca tcgtcccggc
    13141 cgccacccgc ggcgggcaac gcaacctgtt cacggtcggt gccgttccgg aggtggcgca
    13201 gccgtggtcc agttacgcac cgagcgtggt caccctgccc gacggtgcgg tgaagctgtc
    13261 gacgaagttc acccgactca ccggccgctc accgatcctg ctggcgggga tgacaccgac
    13321 gaccgtggac gccaagatcg tcgcggccgc ggccaacgcc gggcactggg ccgaactggc
    13381 cggcggcggc caggtgaccg aagagatctt caccaagcgc atcgaggagc tgcagacgct
    13441 gcttgaacca ggccgcaccg ttcagttcaa ctcgctgttc ctcgacccgt acctgtggaa
    13501 gctgcagatc ggcggcaagc gcctggtgca aaaggcccgc cagtcgggcg caccgatcga
    13561 cggcgtcgtc gtttccgccg gcatcccgga tttggaagag gccgtcgacc tcatcgacga
    13621 gctgaacagc gtcggcatca cccacgtcgt gttcaagccc ggcaccgtcg agcagatccg
    13681 ctcggtgatc cggatcgccg ccgaggtgcc caccaagccg gtgatcgcac acatcgaggg
    13741 cggacgcgcg ggcgggcacc actcgtggga ggacctcgac gacctgcttc tggccaccta
    13801 ctcggagctg cgggcgcggt ccaacatcac ggtctgcgtc ggtggcggca tcggcacacc
    13861 cgagcaggcc gccgaatatc tcaccggcac gtgggcggcg cgacacaatt tcccgctgat
    13921 gccgatcgac ggcatcctgg tcggcaccgc ggcgatggcc accaaggagg ccaccacctc
    13981 accgcaggtc aagcagatgt tggtggccac gcaaggcaca gatcgctgga taggcgccgg
    14041 aaaagcccag ggcggcatgg catctagccg cagccagctc ggtgccgaca tccacgagat
    14101 cgacaacgcg gcgtcgcggt gcggccggct gctcgatgag gtggccggcg acgccgacgc
    14161 ggtggctgcc cgccgcgacg agatcatcgc tgcaatggcc aacaccgcca agccgtactt
    14221 cggcgacgtc ggtgagatga cctacctgca gtggctgcgg cggtacgtgg aattggctat
    14281 cggagaaggc aattcgactg ccgacgccgc cgcgccgggc agcccgtggc tggccgacac
    14341 ctggcgcgac cgcttcgagc agatgctcaa gcgcgccgaa gcgcgtttgc atccaaagga
    14401 tttcgggccg atcgagaccc tattctgtga cgaggagttg ttggaacggc ccgacgacgc
    14461 gatcgtcgcg ctgcagtctc gctatcccga cgccgagacg atccagttgc atccggccga
    14521 cgtgccgttc ttcgtcgagc tgtgcaagac gctgggcaag ccggtcaatt tcgtcccggt
    14581 catcgacaag gacgtgcgcc ggtg
//
LOCUS       DASWBF010000146         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_600796,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000146 DASWBF010000000
VERSION     DASWBF010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600796"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VF874_04935"
     CDS             complement(<1..1447)
                     /locus_tag="VF874_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEX9175456.1"
                     /translation="MFTLVLHTHLPWLAHHGRWPVGEEWLYQSWSAAYLPLMRVLRRL
                     ASEDRQGLLTLGITPVVAAQLDDPYCLDGMHHWLANWQLRALEATTLPNPLREFGIRE
                     YAEAGEALDDFATLWQHGASPLLRELIDTGAIELLGGPLAHPFQPLLNPRLREFALRE
                     GLADAQQRFAHTPAGIWAPECAYAPGMENDYAAAGIRHFMVDGPSLHGDTALGRPVDG
                     SDVVAFGRDLAVSYRVWSPKSGYPGHAAYRDFHTLDHHTGLKPARVTGRNVPSDDKAP
                     YDPARAERAVNGHVADFVDIVRQRLVSETERLGRPAHVIAAFDTELFGHWWYEGPMWL
                     ERVLRALPAAGVRIGTLSDAAAAGFVGAPVQLPPSSWGSGKDWQVWAGEQVADLVQLN
                     AEVVDTALTTVDKALEQTDSPHARDFVADQILRETLLTVSSDWPFMVSKDSAADYARY
                     RAHLHAHATREIAGTLASGRRDAAQRLADGWN"
     gene            complement(1465..>1844)
                     /locus_tag="VF874_04940"
     CDS             complement(1465..>1844)
                     /locus_tag="VF874_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9175457.1"
                     /translation="RVLRPGGLLLMSTPNRITFTPGSDTPVNPFHTRELNASELTELL
                     AGGGFTVESIAGVFHGSRLVALDAKHGGSIIDAQVQRVLADAPWPAELLTDVASVTVD
                     DFELTSDRDIDDSLDLVAIAVRP"
BASE COUNT          297 a          641 c          630 g          276 t
ORIGIN      
        1 gattccagcc gtcggccagc cgctgcgcgg cgtcgcggcg cccagatgcc aacgtgccgg
       61 cgatctcccg cgtcgcatgc gcgtgcagat gcgcgcggta gcgggcatag tcggcggccg
      121 agtccttgct caccatgaac ggccagtcgc tcgacaccgt gagcagcgtc tctcgcagga
      181 tctgatcggc gacgaagtca cgggcgtgcg gcgaatcggt ctgctccagc gccttgtcca
      241 ccgtcgtcaa cgcggtgtcg accacctcgg cgttcagctg caccagatcg gccacctgct
      301 cgccggccca cacctgccag tccttgccgg aaccccaaga gctgggcggc aattggacgg
      361 gtgcgccgac gaacccggcg gccgcagcgt cgcttagcgt gccgatccgc acgcctgcgg
      421 caggcagtgc ccgcagcacg cgctccagcc acatcggccc ctcgtaccac cagtggccga
      481 acagctccgt gtcgaacgcg gcgatgacat gcgctggcct gccgagccgt tcggtttcgg
      541 agacgaggcg ctgacggacg atgtcgacga aatcggcgac gtggccgttc accgcccgct
      601 ctgcgcgcgc tgggtcgtac ggcgccttgt cgtccgaggg gacgttgcgt ccggtcacgc
      661 gggccggctt gagtccggtg tggtggtcaa gggtgtggaa gtcgcggtac gcggcgtggc
      721 cggggtagcc ggacttcggt gaccacaccc ggtagctcac agccaggtcg cggccgaacg
      781 cgacgacatc ggacccgtcg accggccggc caagcgcggt gtcgccatgc aacgacggac
      841 catcgaccat gaagtgccgg attcccgctg cggcgtaatc gttttccatg cccggcgcgt
      901 acgcgcactc cggcgcccag atgcccgcgg gggtatgagc gaagcgttgc tgcgcgtcgg
      961 ccagtccctc gcgcagcgcg aactcgcgca gccgcgggtt cagcagcggc tggaaagggt
     1021 gggccagcgg accgccgagc agttcgatag cgccggtgtc gatcagttcg cgcagcagcg
     1081 ggctggcgcc gtgttgccac agcgtcgcga aatcgtccag cgcctcgccg gcctcggcgt
     1141 actcgcgaat cccgaactcc cgcaacgggt taggtagtgt ggtggcctcc agcgcacgca
     1201 gctgccagtt ggccagccag tggtgcatgc cgtcaaggca gtacgggtcg tcgagctggg
     1261 cggccaccac cggggtgatg cccagcgtaa gcaggccctg gcggtcttca ctcgccaacc
     1321 gccgcagcac ccgcatcagc ggcaggtacg cggcagacca cgactggtac agccactcct
     1381 caccgacggg ccagcggccg tggtgggcca gccacggcag atgggtgtgc aggaccaggg
     1441 tgaacatgcc cggtgtgttt tcggtcacgg ccgcaccgcg atcgccacca gatccaggct
     1501 gtcgtcgata tcgcgatcgg aggtcagctc gaagtcatcg acggtcaccg acgccacgtc
     1561 ggtgagcaac tccgccggcc agggggcgtc ggccaacacg cgctgcacct gcgcgtcgat
     1621 gatcgagccg ccgtgcttcg cgtccaaagc gaccagccgc gaaccatgga acaccccggc
     1681 tatcgattca actgtgaagc cgccgccggc cagcaactcc gtcagctcag aagcgttgag
     1741 ctcccgcgtg tggaacgggt tgaccggagt gtcgctgccg ggggtgaacg tgatcctgtt
     1801 cggcgtcgac atcagcagca ggccgcccgg ccgcaacacc cgca
//
LOCUS       DASWBF010000147         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_15098, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000147 DASWBF010000000
VERSION     DASWBF010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15098"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VF874_04945"
     CDS             complement(<1..474)
                     /locus_tag="VF874_04945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175458.1"
                     /translation="MSTGRCVGKVALVTGSSRGLGKAIAQRLAREGATVALTARTMDP
                     DPKYHGSLRQTLDEITGDGGSAIAMQADLSQPEHRDRLFGEVVERVGAPDILVNNAAV
                     TVLRPLDTVPERRVRLMIEMHVLAPLHLTQLAIPGMRERGRGWVLNVTSVGGDLPE"
     gene            504..1364
                     /locus_tag="VF874_04950"
     CDS             504..1364
                     /locus_tag="VF874_04950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9175459.1"
                     /translation="MELSGVGVWSSQLRYGDQAEAAEAAAELEELGYSALWIPDVGGP
                     VLDSVQHLLRSTQRTVIATGILNLWMHEPADVASSYASLTAAHGDRFLLGIGVSHAPL
                     IDSKEPGRYRKPLAATRSFLDGLDSAERPVPVDRRVLAALGPKMLELSATRAAGAHPY
                     LVTPEHTASARETLGTGPLLLPEQTVILCDSRDVARPIGTEWLRSYLALPNYANNLLR
                     LGFSPDELSSVSDRLFDAIIAWGDEDAIVRRVREHQEAGADHVCIQALEADPRAFPRE
                     QWRRLAAALH"
     gene            complement(1368..2273)
                     /locus_tag="VF874_04955"
     CDS             complement(1368..2273)
                     /locus_tag="VF874_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9175460.1"
                     /translation="MTPTVAAGAQGRQSPSPPTDRVVAVMQLLGAEPHRAFTLAEIGR
                     VLGISRATGHAILSTLASHDWVVRDPETGGYAWGPAIAALARPAGSAARLFRTRLIEL
                     ADAIDMQVLLVRRDGTALVVVDVVGDSLTGAPIRPGFTVPLVAPFGREFVAWGGESAQ
                     QAWLNRLGTASSKFRARMGKVLADIRRRGFAVERLSPEWLRVHTALQALSGDGNVDVV
                     TTRLAATIADVTVVDFLDTELARGEHDIATVMTPVFDADGVVTMSVNAIPFGPLSGPA
                     IGRLGGQLRDAAHDIEGLIARYGDA"
     gene            2325..3569
                     /locus_tag="VF874_04960"
     CDS             2325..3569
                     /locus_tag="VF874_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX9175461.1"
                     /translation="MGAVHRERPGADDLAAATGAPAIPLGDDIWMSPGVSNSYAVGTE
                     DGRVIINAGVVFEGSPHRQAFADVPGPTRRVVVTQGHADHWGGIGSLRDPDTDVVMHS
                     NYRYWRDDNERLAGYRMRNTSFAFHKFTDAFLEHFKTIDPATIDFSFPEPAVTFDQHL
                     EFEVGGRRFVLTWTPGGETTDALVVWLPDDRILFTGNLFGPLFGHVPNLMTIRGDRYR
                     DPVLYIESLNTVLSFAPQRIITGHFDPIEGADRIAEEVTAMRDAMQSVHDQTVEGMVA
                     GTDVHTLMRDVRIPPHLDVGEGYGKTSWNVRAIWEMYAGWFYHRSTTELYHVPPTAIS
                     GDLIEAAGADRLIEAAKARLSRDEPLEALHLTDIVLGVDAGNTDARTVAVAAHEKLLA
                     GKQNFWEKAWLRNTIEELRTTP"
     gene            3566..4315
                     /locus_tag="VF874_04965"
     CDS             3566..4315
                     /locus_tag="VF874_04965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175462.1"
                     /translation="MNTANFDFSGANVLVTGGTSGIGNAIARSFADSGATVTITGTRA
                     ATADYDDDLSAFTYRRCQIADPASVDALVESLGDLDVLVNNAGGPYPGGQDEWDPDGY
                     SASVTQNMFGPMRLTMRCHDRLKASTASGGASVVTILSMSAFRSAIFVPGYASSKAGL
                     LALTMNLSRRWAGDGIRVNAVAPGLIDTRMTNPALSIPEVTDVEIGFHTPLGRWGTPQ
                     DCMGAVLFLCSDSASFITGTSIAVDGGYLTV"
     gene            4336..>5257
                     /locus_tag="VF874_04970"
     CDS             4336..>5257
                     /locus_tag="VF874_04970"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9175463.1"
                     /translation="MSECDSVSTPDDVLTLAQQRTGLTDVDSDSWRPGLAILLEELNT
                     SSVVTPSGRDHQIDSYVKALSNRLQVHDYVRRNPDVLDEKIEHPLVTLGMPRTGTTVI
                     SYLLDQDPARRSLLHWECVHPVPPATTETLRTDPRCVALLEEQKQIIKFVKEANMPMV
                     HWEDADGPTEDMFIHNADFKALSWDAWSPTARYADWLINEADMTSTYEYQKRVLQVLQ
                     SKAPGIWSLKMPSHSVHIEALLKVFPDVRMVCAHRDPYKATGSLCNMWMVPQKMAMNE
                     ADVDRNALGRKAMMQMKAHVERPLRARERIG"
BASE COUNT          935 a         1770 c         1689 g          863 t
ORIGIN      
        1 ctcaggcaga tccccgccga ccgaggtgac gttgagcacc cagccgcggc cgcgttcgcg
       61 cattcccgga atggccaact gggtcagatg cagcggcgcc agcacgtgca tctcgatcat
      121 caggcgcacc cgccgctccg ggacggtgtc gagcggacgc aggactgtca cggcggcatt
      181 gttgaccaaa atgtccggtg ccccaacgcg ttcgaccacc tcgccgaaaa gccggtcccg
      241 atgctcaggc tgtgacagat cggcttgcat ggcgatcgcc gagccgccgt cgccggtgat
      301 ctcgtcgagc gtctgccgca gcgacccgtg atatttcggg tcggggtcca tggtgcgtgc
      361 ggtcagcgcg acggtggctc cttcgcgggc caaccgctgc gcgatcgcct tgcccaggcc
      421 ccggctgctg ccggtcacca gcgcgacctt gccgacgcac cttcctgtac tcataaccag
      481 agacgcacgt taccgtcaga ggcgtggaac tgtctggtgt tggggtatgg agttcgcaac
      541 tgcgctacgg cgaccaggcc gaggcggccg aggcggcagc ggaactcgag gagctcgggt
      601 attcggcgct gtggattccc gacgtcggcg gaccggtgct cgactcggtg cagcacctgc
      661 tgcgctccac ccagcggacc gtgatcgcca ccggcatcct caacctttgg atgcacgagc
      721 cggccgacgt cgcgagttcc tatgcatcgc tgacggcggc ccacggcgac cggttcctgc
      781 tgggtatcgg tgtcagccat gcaccactca tcgattccaa agagcccggt cggtaccgca
      841 aaccgctggc ggcgacgcgg tcgttcctcg acgggctgga cagcgcggaa aggccggtgc
      901 ccgtcgaccg gcgtgtgctc gccgcgctgg gccccaagat gctggagctg tcggccaccc
      961 gtgcggcggg agcccatccc tacctggtga cgcccgagca cacggcgagc gcccgcgaga
     1021 cgttggggac cgggccgctg ctgctgccgg agcagacggt catcctgtgc gacagccgcg
     1081 acgttgcccg gcccatcggg acagagtggc tgcggtccta cctggcgttg ccgaactacg
     1141 cgaacaacct tctgcgcctt ggcttctccc ctgacgaact gtcctcggtg agcgaccggc
     1201 tgttcgacgc gatcatcgcc tggggagacg aggacgcgat tgtgcgcagg gtgcgagagc
     1261 accaggaggc cggcgccgac cacgtctgca tccaggcatt ggaagccgac cccagggcat
     1321 tcccgcgtga acagtggcgt cgcctcgccg ctgcgctgca ctaaacgtca ggcgtcgccg
     1381 taccgagcga tcaagccctc gatgtcgtgc gcggcgtcac gcaattgccc accgaggcgc
     1441 ccaatagccg ggccgctgag cggaccgaac gggatcgcat tgaccgacat cgtcacgacg
     1501 ccatcggcat cgaagaccgg ggtcatgacc gtcgcgatgt cgtgctcgcc ccgcgccagt
     1561 tccgtgtcga ggaagtcgac gacggtgaca tcggcgatcg tggcggccag ccgggtcgtg
     1621 acgacgtcga cgtttccgtc gccgctgagc gcctgcaggg cggtgtgcac gcgaagccac
     1681 tcgggactga gccgttcgac ggcgaagccg cggcggcgga tgtcggcgag caccttgccc
     1741 atccgcgccc ggaacttcga gcttgcggta ccgagccggt tgagccatgc ctgctgtgct
     1801 gactcgccgc cccacgcgac gaattcccgg ccgaacggcg ccaccagcgg caccgtgaac
     1861 ccgggccgga tcggcgcgcc ggtcaagctg tccccgacca catccaccac gaccaacgct
     1921 gtgccgtccc gccggacgag cagcacctgc atgtcgatcg cgtcggcgag ttcgatcagc
     1981 cgagtgcgga acaaccgggc cgcgctgccc gccggccggg ccagcgccgc gatcgccggt
     2041 ccccatgcat agccgccggt ctccgggtcg cggacgaccc agtcgtgcga ggcgagcgtc
     2101 gacaggatcg catgcccggt ggcacggctg atgccgagca cgcgcccgat ctcggcgagc
     2161 gtgaacgcgc ggtgaggttc cgcgcccaag agttgcatca cggcgacgac ccggtccgtc
     2221 ggcggggacg gcgattgacg gccctgggcg ccagccgcta ccgtaggtgt catattctcg
     2281 aatactatgt cgattattcg acgcacgcaa tgcatggagt tgtgatgggt gctgttcatc
     2341 gcgagcggcc cggcgccgac gacctggcgg cagccaccgg tgctcccgcg atacccctcg
     2401 gcgacgacat ctggatgtcg ccgggagttt cgaattccta cgccgtaggc accgaggacg
     2461 gccgcgtcat catcaacgcc ggagtggtgt tcgaagggtc cccgcaccgg caggcgttcg
     2521 cagatgttcc gggcccgacc cgacgcgtcg tcgtcaccca gggccacgcc gatcattggg
     2581 gcggcatcgg atctctgcga gaccccgata ccgacgtcgt catgcactcc aactaccgct
     2641 actggcgcga cgacaacgag cggctcgctg gataccggat gcgcaacacc tcgttcgcct
     2701 tccacaagtt caccgacgcg ttcctggagc acttcaagac catcgatccc gccacgatcg
     2761 acttctcctt tcccgagccc gcggtgacct tcgaccagca cctcgagttc gaggtcggag
     2821 gtcgccgttt cgtgctgacg tggacgccgg gcggtgagac gacggacgcg ctggtcgtgt
     2881 ggctgcccga tgaccgaatc ttgttcaccg gcaacctgtt cgggccgctg ttcgggcacg
     2941 tacccaactt gatgacaatc cgcggcgacc ggtaccgcga ccccgtgctg tacatcgagt
     3001 cgctcaacac cgtgctctcg ttcgctccgc aacgcatcat caccggtcac ttcgatccga
     3061 tcgagggcgc cgaccgaatc gcggaagagg tcacagcgat gcgtgatgcg atgcagtcgg
     3121 tgcacgacca gaccgtcgag gggatggtcg ccggaaccga cgtgcacacg ctgatgcgtg
     3181 acgtccggat tccgccgcat ctcgacgtcg gtgagggcta cggcaagacc tcctggaacg
     3241 tccgcgccat ctgggagatg tatgccggct ggttctatca ccgctccacg accgagctgt
     3301 atcacgttcc gccgaccgcg atttcgggtg acctcatcga ggctgcaggc gctgatcggc
     3361 ttatcgaagc cgcgaaggcg cggctgagcc gcgatgagcc gctggaggcg ctgcacctga
     3421 ccgacatcgt tctcggcgtc gacgccggca acaccgacgc tcgaacggtt gccgttgcag
     3481 cgcacgaaaa gctactggca ggcaagcaga acttctggga gaaagcgtgg ctgcgcaaca
     3541 ccatcgagga gctgaggacg acaccatgaa caccgccaac ttcgacttca gcggcgccaa
     3601 tgtcttggtc acaggcggaa ccagcggcat cggcaacgcg atcgcgcgct cgttcgccga
     3661 ctcgggcgcc accgtcacca tcactggcac ccgcgcggcg acggccgact acgacgacga
     3721 cctcagcgcg ttcacgtacc ggcggtgcca gatcgccgac ccagcgtcag tcgacgccct
     3781 cgtcgagtca ctcggcgatc tcgacgtcct cgtgaacaac gccggcggtc cttatcccgg
     3841 cggacaagac gaatgggatc cggacggcta ttccgcctcg gtcacccaga acatgttcgg
     3901 gccgatgcgg ctcactatga ggtgtcatga ccggctgaaa gccagcaccg cctcgggcgg
     3961 cgcgagtgtc gtgaccattc tctcgatgtc ggcgttccga tcggcgatct tcgtgccggg
     4021 ctacgcgtcg tcgaaagccg gccttctcgc attgacgatg aacctctccc gacgctgggc
     4081 cggggacggc atccgcgtca acgcggtcgc acccgggttg atcgacacgc gaatgaccaa
     4141 tcccgcgttg agcattcccg aagtcacgga tgtggaaatc ggattccaca ctccgctggg
     4201 tcgatggggc accccgcaag actgcatggg cgcggtgctg tttctgtgca gcgactccgc
     4261 ctccttcatc accggtacca gcatcgccgt cgacggcgga tacctgacgg tctgaacgaa
     4321 gcgaggaaat accaagtgag cgaatgcgat tccgtctcga ccccggacga tgtcctgacg
     4381 ttggcccaac agcgcaccgg gctgaccgac gtcgactccg actcgtggcg cccgggcctg
     4441 gccatcctgc tcgaggaact caacacgtcg tcggtggtca caccctccgg gcgcgatcat
     4501 caaatcgata gctacgtcaa ggcgctcagc aaccggctgc aggtgcacga ctatgtgcgg
     4561 cgaaatccgg acgtcctcga cgagaagatc gagcatccgt tggtgactct ggggatgcca
     4621 cgcaccggca ccaccgtcat cagttacctg ctcgaccagg atcccgcgcg acggtccctg
     4681 ctgcactggg aatgtgtgca tccggtaccg ccagccacga cggagacgct gcgcaccgac
     4741 cctcggtgcg tggccctgct cgaggagcag aagcagatca tcaagttcgt caaagaggcg
     4801 aacatgccga tggtgcactg ggaggatgcg gacggcccga ctgaggacat gttcatccac
     4861 aacgctgatt tcaaagcgct gtcgtgggac gcgtggtcac cgacggccag atacgccgat
     4921 tggctcatca acgaagccga catgaccagc acgtatgagt atcagaaacg ggtgctgcag
     4981 gtgttgcagt cgaaagcccc cggcatttgg agtttgaaga tgccgtcgca ctccgtgcac
     5041 atcgaggcgc tactcaaggt attcccggat gttcggatgg tctgtgcgca tcgcgacccg
     5101 tacaaggcga ccggctccct gtgcaacatg tggatggtgc cacagaagat ggcgatgaac
     5161 gaagccgacg tcgaccgaaa cgcgttgggc cgcaaggcga tgatgcagat gaaagcacac
     5221 gtcgagcgtc cgcttcgggc ccgcgagcgc atcggtg
//
LOCUS       DASWBF010000148        10127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1201113,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000148 DASWBF010000000
VERSION     DASWBF010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10127
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201113"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1735)
                     /locus_tag="VF874_04975"
     CDS             complement(233..1735)
                     /locus_tag="VF874_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX9175464.1"
                     /translation="MWRRCRTAAALLSVVALLAALVGCSRDNREERRTAPAAADYALS
                     LREKVTTDAMFAHLEQLQQIADANGGNRAVGSPGYDASLDYVAKTLRVKGFDVQIPEF
                     DFRKFDPGPVSLTVNGATVSAQVLEYSVGTGPGGVSGPLIVAPGPPGDDTPGCSASDY
                     DGLPVSGAVVLIDRGYCQFSSKEEAAAQRGAVALIVADNVDEEKMSATLGARTEVKIP
                     VIGVSKADGAKLRTQPGPTAIKVDAKVTNIKARNVIAQTKTGSTQNVVMAGAHLDSVP
                     EGPGINDNGSGVAAVLETAVQMGSSPPIQNAVRFAFWGAEEAGLIGSMNYIESLDADG
                     LKDIALYMNFDMIASPNPGYFTYDGDQSSAQGPDESTPRVPEGSAGIERTLVSYLTNA
                     GKTAEDTSFDGRSDYDGFTKAGIPSGGLFSGAEEKMSAEQAELWGGKADQPFDPNYHK
                     PTDTLQQINRTALGINGPGVAYALGLYSQDIAGRNGVPVRDDRTRHVLKD"
     gene            complement(1759..2502)
                     /locus_tag="VF874_04980"
     CDS             complement(1759..2502)
                     /locus_tag="VF874_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9175465.1"
                     /translation="MTVAAERIKLSTTRSPIWSAVIAAMLSLGIAALQPAATLSDEPL
                     PPERAAVGVVTFAVPVLMILAAMTITGEYRSGMIRTTFMATPHRSRVLGAKALITAVF
                     AGVSAAGMVIASLLVARALANGQVGARLPLTHAEAWRPVGAVGLYAALGAVLAVGLAV
                     LLRYAAAVIAVLLLMPFVIEPLLGSMPRIGEHVGPLLPFANAWAFTGVPWFGAFPPSW
                     GPLGSLLYFTAVVAAIYLAAVFVINRRDP"
     gene            complement(2499..3410)
                     /locus_tag="VF874_04985"
     CDS             complement(2499..3410)
                     /locus_tag="VF874_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9175466.1"
                     /translation="MIELRGLTKTFGSTRAVDDLTCTIEPGVVTGFLGPNGAGKSTTM
                     RMILGLDRPTAGTATIGGHRYHDLPHPLRTVGALLDAKQVHPNRTARNHLRWMVASNR
                     LAAGRADEVLAMVGLTEVAGKKAGTFSLGMSQRLGIAAALLGDPPVLLFDEPVNGLDP
                     EGIRWVRTLMRTLADQGRTVFVSSHLLAEMANTADQLVVIGRGRLIAATTMSEFVSRY
                     GADTVRVRSPQSGTLRDVLTAAGMQVDGDEVALLVSGGSLEQVGELAAGHRITLHELS
                     AQRASLEEAYMKLTDDSVDYRASGVPS"
     gene            complement(3407..4195)
                     /locus_tag="VF874_04990"
     CDS             complement(3407..4195)
                     /locus_tag="VF874_04990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX9175467.1"
                     /translation="MTDRYVALGSSMAAGPGITPRAGGAPWPSGRSARNYPHLVATQL
                     GVDLVDVTYSGATTAHVLRDKQFGAPAQVDALDGSEILVTVTIGGNDVGYIPLLTLAC
                     LPWPAQWVPVLGCRIRNLRDRAARDDALDDVAESLREVGDTIRRRSPQARVLFVDYLT
                     VLPPAGRSASPLSNADSDLGRHIATTLERLTAEAADATGCELVKAGEASRAHHAWSSD
                     PWTTRFHLPWPRRPAPMHPNAAGMRAVAELVTAQVSDGLPSWAP"
     gene            complement(4210..4418)
                     /locus_tag="VF874_04995"
                     /pseudo
     CDS             complement(4210..4418)
                     /locus_tag="VF874_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
     gene            4490..5890
                     /locus_tag="VF874_05000"
     CDS             4490..5890
                     /locus_tag="VF874_05000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX9175468.1"
                     /translation="MRSNAVLDRDGVLAVVERYRGAVTELVNLPLDALGVADLFAVLD
                     ATETGRCQTPVIEHQAINRIAAQATPEQIGRSLKKTLAARLRIRPGEARRRIADAEML
                     GARTTITGEPLPAQWPATAAAQRAGQINTAQVAEIGRFFHHLPSWVDESARARAEADL
                     AEQATKFGPDELRVLADKLADCLNPDGTFTDEDRARRRGITIGRQDIDGMSKITGWLT
                     PEARASLDAVLSKWAAPAMCNPADESPTVDGRPSEQAINSDMRSPAQRNHDALAAMCR
                     STLASGQLGSHHGLPVSIVVSTTLQELESAAGIAHTGGGSWLPMPDVIRMASHARHYL
                     RIYDTHTSRELYLGETKRIATPAQRIVLHAKDRGCTRPGCTAPGYLSEVHHVNEWAVH
                     HRTDIDDLTFACGPCHKLLDDDGWTTRKNADGVTEWIPPPHLDFGKPRTNGYWHPQRY
                     FRLDDNDDNGDDGDPP"
     gene            complement(5914..6693)
                     /locus_tag="VF874_05005"
     CDS             complement(5914..6693)
                     /locus_tag="VF874_05005"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX9175469.1"
                     /translation="MADKLTIAGRSFTSRLIMGTGGASNLAVLEEALVASGTELTTVA
                     MRRVDAGGGTGVLELLTRLSITPLPNTAGCRSAAEAVLTAQLAREALQTDWVKLEVIA
                     DERTLLPDAVELVRAAEQLVDDGFVVLPYTNDDPVLARRLEDIGCAAVMPLGSPIGTG
                     LGIANPHNIEMIVDAAGVPVVLDAGIGTASDAALAMELGCDAVLLASAVTRAADPPAM
                     AAAMAAAVTAGHLARHAGRIPKRFWARASSPGLPWADGQGG"
     gene            complement(6686..6883)
                     /gene="thiS"
                     /locus_tag="VF874_05010"
     CDS             complement(6686..6883)
                     /gene="thiS"
                     /locus_tag="VF874_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX9175470.1"
                     /translation="MIVTVNDERVQVDDDTTVAALLSRLGFPDKGVAVALDWAVLPRS
                     GWGTTLSDGARLEVVTAVQGG"
     gene            complement(6880..7884)
                     /gene="thiO"
                     /locus_tag="VF874_05015"
     CDS             complement(6880..7884)
                     /gene="thiO"
                     /locus_tag="VF874_05015"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEX9175471.1"
                     /translation="MSNESIAVIGGGVIGLSVARRLALSGRTVRVHRNPADPGASWVA
                     GGMLAPHSEGWPGEERLLQIGLESLALWHRGFLDESVITARESLVVAVDRADAADLCT
                     VGEWLAAQGHPVTLTTNARDLEPLLAQGIRHGFRATTELAVDNRAVVQALAGHCERLG
                     VAWAPGVEDLAEVADADAVVLANGIDVPALWPSLPIRPVKGEVLRLRWRPGCMPVLQR
                     VIRARVHGRQVYLVPRADGVVVGATQYEHGRDTAPAVTGVRELLDDACAVMPALGEYE
                     LAECAAGLRPTTPDNLPIVGRLDDRVLVAAGHGRSGFLLAPWTAEKILAELDALVGAT
                     "
     gene            7907..8569
                     /gene="thiE"
                     /locus_tag="VF874_05020"
     CDS             7907..8569
                     /gene="thiE"
                     /locus_tag="VF874_05020"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX9175472.1"
                     /translation="MREDRLAEARLYLCTDARRERGDLAEFADAALAGGVDIIQLRDK
                     GSVGEQRFGPLEARDELAALEVLANAARRHGALLAVNDRADIAKAAGADVLHLGQDDL
                     PLQVAREIAPQTLIGRSTHDTVQARTALTEDVDYFCVGPCWPTPTKPGRPAPGLDLVR
                     AVASMRTDKPWFAIGGIDLQRLPQVLDAGARRVVVVRAITAADDPAQAARDLSAALAA
                     AG"
     gene            complement(8541..9035)
                     /locus_tag="VF874_05025"
     CDS             complement(8541..9035)
                     /locus_tag="VF874_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014876872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9175473.1"
                     /translation="MRGDGDGWVMSDSGAMFWGRHGAAGLLLRAPRADGGRVVLLQHR
                     AVWSHQGGTWALPGGARDSHETAEETAVREAAEEAGLAAEHVVVRAAVVTATASGSHW
                     TYTTVVADAPHPLDTMPNFESVELRWVVEEEVGDLPLHPGFAASWHTLLELSRVTPLL
                     QVPR"
     gene            9138..>10127
                     /locus_tag="VF874_05030"
     CDS             9138..>10127
                     /locus_tag="VF874_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175474.1"
                     /translation="MTVELAHPSTEPAPSQAAVDPAHPRWWFWTTTPGRILVSGVVLI
                     ALVTLSAFGTASTIDRRQEALTTVLNHTEPLAFAAGQLYTTLSVADAAAATAFIAGAE
                     PREVRQRYEQAITDAAVALTQASSGLTDQPMLELLGRINARLAVYTGLIETARTNNRA
                     GNPVGSSYLSEASSLMQQQILPDAQRLYDETSARVDAETTASTNIPTLLIVLVAATLL
                     FGAYSNRWLAQKTQRRLNFGFVAGGLAVLIMVMWVGTVLAISTTDSRSAKGTAADSLK
                     TITNLAIMAQQARADETLALIRRGDEDIRKQSYYQRIDTMHQLLDEYTKRKDAI"
BASE COUNT         1640 a         3666 c         3346 g         1475 t
ORIGIN      
        1 ccgccgcagc cgcgccattg ccgggctctg ccatgaactc accgccaccc aaacccgcta
       61 ccccggcacc gacctcaaga tcaaatacag cgtcaaaggc cacgacatca cataaacgat
      121 cccactatgt cgggagtcct gagcccgccc cacgacccta caacccatcc gagccaatca
      181 cttgacacag agaggcgccg cagagtcgac gaaagacgca agagcgcccg tctcagtcct
      241 tcagcacgtg ccgggtgcgg tcgtcgcgaa ccggcacgcc gttgcgcccg gcgatgtctt
      301 gcgagtacag ccctaacgca tacgccacgc cggggccatt gatgcccagc gcggtccggt
      361 tgatctgctg caacgtgtcg gttggcttgt ggtagttggg gtcgaacggc tggtccgcct
      421 tgccgcccca cagctccgcc tgctcggcgc tcatcttctc ttcggcaccg gagaacaggc
      481 cacccgacgg aattccagcc ttggtgaagc cgtcatagtc ggagcgcccg tcgaaagacg
      541 tgtcctcggc ggtctttccg gcattagtca ggtaggagac cagcgtccgc tcgatgccgg
      601 ccgatccctc cggcacccgc ggcgtgctct cgtcgggtcc ctgtgctgac gattgatcgc
      661 cgtcgtacgt gaaatagccg gggttcggcg aggcgatcat gtcgaagttc atatagagcg
      721 cgatgtcttt gagcccgtcg gcgtcgaggg actcgatgta gttcatggac ccgatcaagc
      781 cggcctcttc ggccccccag aacgcgaacc ggacggcgtt ctggatcggc ggtgagctac
      841 ccatctgcac cgccgtttcg agcaccgccg ccactccgga cccgttgtcg ttgatgccgg
      901 ggccctccgg cacgctgtcc aggtgcgctc ccgccatcac cacgttctgc gtggaaccgg
      961 tcttggtttg cgctatcacg ttgcgcgcct tgatgtttgt cacctttgcg tcgaccttga
     1021 tcgcggtcgg ccccggctgg gttctcaact tggccccgtc ggccttgctg acgccgatca
     1081 ccggaatctt gacttctgtc cgagcgccca gggtggcgct catcttctct tcgtccacgt
     1141 tgtcggcgac gatcagcgcc accgcccctc gctgcgctgc cgcctcctcc ttgctggaga
     1201 actggcagta gccgcgatcg atcagcacta ccgcaccgga gaccggcagc ccgtcgtagt
     1261 ccgacgcgga gcaccccggg gtgtcgtcgc ccggtggacc gggcgcgacg attagcggac
     1321 cgctgacccc gcccggcccg gtacccacgg agtactcgag cacctgcgcg gaaaccgttg
     1381 cgccgttgac ggtcagcgat accgggcccg gatcgaactt acggaaatcg aactccggga
     1441 tctgcacgtc aaaacctttg acacgcaagg ttttcgcgac gtaatcgagg ctggcatcgt
     1501 aaccggggct gccaacggcc cggttgccgc cgttcgcgtc ggcgatctgc tgcagctgct
     1561 ccaggtgcgc gaacatcgcg tcggtggtga ccttctcacg cagcgacagc gcgtagtccg
     1621 cggccgccgg cgcggtccgc cgctcctcgc gattgtcgcg cgaacatccc acgagcgcag
     1681 cgagcagcgc caccacgctc agcagcgcgg ctgccgtcct gcatcttcgc cacaccgcgc
     1741 aaaccttagc cgccggcatc agggatcgcg ccgattgatc acgaacaccg cggccaagta
     1801 gatcgccgcg acgacagcgg tgaagtacag cagcgaaccc agcggtcccc acgatggcgg
     1861 gaaggccccg aaccacggca ccccggtgaa cgcccacgcg ttcgcgaacg gcaacagcgg
     1921 gcccacgtgc tctccgatgc gcggcatcga acccagcagc ggttcgatca cgaacggcat
     1981 cagcagcagc accgcgatca ccgcggccgc gtaccgcagc agtaccgcga ggccgacggc
     2041 cagcaccgcc cccaatgcgg cgtacaaccc aaccgccccc acagggcgcc acgcctccgc
     2101 gtgcgtcagt ggtaatcgag cgccgacctg cccgttggcc agcgcccgcg ccaccaacag
     2161 cgacgcgatc accattcctg cggcggaaac gcccgcgaag acggccgtga tcaacgcctt
     2221 ggcgcccagc acgcgcgagc ggtgcggggt ggccatgaag gtcgtgcgaa tcatcccgga
     2281 acggtattcg ccggtgatcg tcatcgccgc gaggatcatc agcaccggca cggcgaaggt
     2341 gaccacgccg actgcggccc gctccggggg caacggctca tcggacaggg tcgccgcagg
     2401 ctgaagcgcc gcgatgccca ggctgagcat cgccgcgatc accgccgacc agatcggtga
     2461 tcgggtggtc gacagcttga tgcgctcggc cgcaaccgtc atgacgggac gccgctcgcc
     2521 cggtagtcca cgctgtcgtc cgtcagcttc atgtaggcct cctccagcga cgcgcgctgc
     2581 gcactgagct catgaagggt gatgcggtga ccggccgcca gctcgccgac ctgttcgagg
     2641 ctgccgccgc tgaccagcag cgccacttcg tcgccgtcca cctgcatacc ggcggccgtc
     2701 agcacgtcgc gcagcgtgcc cgattgcggg ctgcggaccc ggacggtgtc ggcgccgtac
     2761 cggctcacga attcgctcat cgtggtcgcc gcgatcagcc tgcctcgccc gatcacgacg
     2821 agctggtcgg cggtgttcgc catttcggcg agcaggtggc tggaaacgaa caccgtgcgg
     2881 ccctggtcgg ccagcgtccg catcagcgtg cgcacccacc ggattccctc cgggtcgagg
     2941 ccgttgaccg gttcgtcgaa gagcagcacc ggcgggtcgc cgagcagcgc cgccgcgatg
     3001 cccagccgct gcgacatgcc caacgagaac gtcccggcct tcttgccggc cacctccgtc
     3061 aagcccacca tggcaagcac ttcgtccgcg cggccggccg ccagccggtt gctggccacc
     3121 atccagcgca gatggttgcg cgccgtccgg ttggggtgca cctgtttcgc gtccagcagc
     3181 gcaccgaccg tgcgcagtgg gtggggcagg tcgtggtagc ggtggccgcc gatggtggcg
     3241 gtcccggcgg tcggccggtc gaggccgagg atcatgcgca tcgtggtgga cttgcctgcg
     3301 ccgttgggcc cgaggaagcc ggtgacgacg ccgggttcga tcgtgcacgt caggtcgtcg
     3361 acggcgcggg tggaaccgaa cgtcttggtc agcccgcgca gttcgatcac ggcgcccatg
     3421 atggcaggcc gtcgctgacc tgtgccgtca ccagctcggc gaccgcccgc atcccggccg
     3481 cgttggggtg catcggcgca ggccggcggg gccacggcag gtgaaagcgc gtcgtccacg
     3541 gatccgacga ccacgcgtgg tgcgcgcggc tcgcctcgcc ggctttgacg agttcgcacc
     3601 cggttgcgtc ggcggcttct gcggtcagcc gctccagcgt cgtggcgatg tggcggccga
     3661 ggtcggagtc ggcattcgac agcggcgacg cgctccgtcc ggccggcggt agcacggtca
     3721 ggtagtcgac gaacagcacc cgcgcctgcg gcgagcgtcg ccggatcgtg tcgccgacct
     3781 cccgcagcga ctcggcgacg tcatcgagtg cgtcgtcgcg ggcggcccgg tcgcgcaggt
     3841 tgcggatccg gcagcccagc acaggcaccc actgggccgg ccacggcagg cacgcgagcg
     3901 tgagcagcgg tatgtagccg acgtcgttgc cgccgattgt tacggtgacc agaatctccg
     3961 aaccgtcgag cgcatcgacc tgcgcgggcg cgccgaactg cttgtcgcgc aggacatgtg
     4021 cggtcgtcgc gcccgagtac gtcacgtcca ccagatcgac gcccagctgg gtggcgacca
     4081 ggtgcggata gttgcgcgcc gagcgtccgg acggccacgg cgcgccgccc gcgcggggtg
     4141 tgattccggg gccggccgcc atcgaactgc ccagcgcgac gtagcgatcc gtcatgtgcg
     4201 ccattgtgcc taccgcagca cgacggtaac cgggcagccg acgcccgcgc gcgactcaac
     4261 cgcgcgtcgg ccgtgacgag agcacagccc agggtttcgg ccagcgccac gtaactcgcg
     4321 tcataggaag aaaggttgtc gcgcagctcc caggtgcggc cgagcagcgg atggatcgca
     4381 tgacgaatca cagccaactt gcgccacgcg ccgagggcag ccagcacggc gggatttcgg
     4441 ctgtggattg ccgcgagctt gtgtcggtgg gcggggatat tattcgctca tgcgttcgaa
     4501 cgcggtgttg gatcgggacg gggtgttggc cgtcgtcgag cgctatcgcg gggcggtcac
     4561 cgagttggtg aacctgccgt tggacgcgct gggggtggct gatctgttcg cggtgctcga
     4621 cgccaccgaa accggccgct gccaaacccc ggtgatcgag caccaggcca tcaaccgcat
     4681 cgccgcgcag gccacccccg agcagatcgg cagatcgctg aagaagacgc tggccgcccg
     4741 gctgcgcatc cggcccggcg aggcgcgccg gcgcatcgcc gacgccgaga tgctgggcgc
     4801 gcgcaccacg atcaccggcg aaccgctgcc ggcgcagtgg cccgccaccg ccgccgccca
     4861 gcgcgccgga cagatcaaca ccgcccaggt ggccgagatc ggccgcttct tccaccacct
     4921 gcccagctgg gtggatgagt ccgcccgagc cagggccgag gcggatctgg ccgagcaggc
     4981 caccaagttc ggccccgacg aactccgcgt gttggccgac aagctggccg attgtttgaa
     5041 cccggacggc accttcaccg acgaggaccg ggcgcggcgg cgcggcatca ccatcgggcg
     5101 ccaagacatc gacgggatgt cgaagatcac cggctggctg acccccgaag cccgcgccag
     5161 cctggatgcg gtgctctcca agtgggcggc cccggcgatg tgcaatcccg ccgacgaatc
     5221 acccaccgtg gacgggcgcc cctccgaaca ggccatcaac agcgacatgc gctccccggc
     5281 ccagcgcaac cacgacgcgc tggccgcgat gtgccgcagc accttggcgt ccggacagtt
     5341 gggcagccat cacgggctgc cggtcagcat cgtggtcagc accacactgc aggagctgga
     5401 gtcagccgcc ggcatcgccc acaccggcgg agggagctgg ctgccgatgc ccgatgtgat
     5461 ccggatggcc agccacgccc ggcactatct gcgcatctac gacacgcaca ccagccgcga
     5521 gctgtatctc ggggaaacca aacgcatcgc caccccggct cagcggattg tgttgcacgc
     5581 caaagaccgt ggctgcacac ggcccgggtg cacggcgccg ggctacctgt cagaggtcca
     5641 tcacgtcaac gaatgggccg tgcaccaccg caccgacatc gacgacctga cattcgcctg
     5701 cggaccctgt cacaagctgc tcgacgacga cggctggacc acccgcaaga acgccgacgg
     5761 cgtcaccgaa tggattccgc cgccccatct ggacttcggc aaacccagaa ccaacggcta
     5821 ctggcacccc caacgctact tccgactcga cgacaacgac gacaacggcg acgacggcga
     5881 ccccccgtag cggcccactt acttcgaggc cgctcaccca ccctgtccgt cggcccacgg
     5941 caacccaggg ctcgacgcgc gcgcccagaa tcgcttcggg atccggccgg cgtgccgggc
     6001 caggtgcccg gcggtgacgg cagcggccat cgccgcggcc atcgccggcg ggtcggcggc
     6061 cctggtgact gcgctggcca gcagcacggc gtcgcacccg agctccatcg ccagtgcggc
     6121 gtcgctggcc gtgccgatgc cggcgtcgag cacgacgggc accccggcgg cgtcgacgat
     6181 catctcgatg ttgtgcgggt tcgcgatgcc cagcccggtg ccgatcgggg agcccaacgg
     6241 catcaccgcg gcacatccga tgtcttccag ccgccgcgcg agcaccgggt cgtcgttggt
     6301 gtagggcagc acgacgaagc cgtcgtccac caattgctct gcggcacgga ctagttcgac
     6361 ggcgtcgggc agcagcgtgc gttcgtcggc gatcacctcg agcttgaccc agtcggtctg
     6421 cagcgcctca cgggccagct gcgcggtgag taccgcctcg gccgcgctgc ggcacccggc
     6481 ggtgttgggc agcggggtga tggacagccg ggtcagcagc tccaacacgc cggtgccgcc
     6541 tccggcgtcg acgcggcgca tcgccaccgt ggtcagctcg gttcccgacg cgaccagcgc
     6601 ctcttcgaga accgcgaggt tgctcgcccc gcccgtgccc atgatcagcc gggacgtgaa
     6661 gctgcggccg gcgatggtga gcttgtcagc caccctgcac cgccgtcacc acctctagtc
     6721 gggcgccgtc ggaaagcgtt gtgccccaac ctgatcgggg cagcacggcc caatctagcg
     6781 ctaccgccac gcctttgtcg gggaatccga gccggctcag cagcgcggcg acggtggtgt
     6841 cgtcgtccac ctgcacacgt tcgtcgttga cggtgacaat catgtggccc ccacaagcgc
     6901 gtccaactcg gcgagaatct tctccgccgt ccatggtgcc aacagaaacc cggaccggcc
     6961 atggccggcc gccaccagca cgcgatcgtc cagacggccc acgatcggca agttgtccgg
     7021 cgtcgtcggc cgcagcccgg ccgcgcactc ggccagctcg tactcgccga gcgccggcat
     7081 caccgcgcac gcgtcgtcga gcaactcccg caccccggtc accgcgggcg ccgtgtcgcg
     7141 gccgtgctcg tattgtgtgg cgccgaccac cacgccgtcg gcacgcggca ccagataaac
     7201 ctgcctgcca tgcacgcggg cgcgaatcac ccgctgcagc accggcatac agcccggccg
     7261 ccaccgtagg cgcagcacct cccctttgac cggccggatc ggcagagatg gccacagcgc
     7321 cgggacgtcg atgccgttgg ccagcaccac cgcgtcggcg tcggcaacct cggcgaggtc
     7381 ctcgaccccc ggcgcccagg cgacgccgag gcgttcgcag tgcccggcca acgcctggac
     7441 caccgcgcgg ttgtcgacgg ccagctcggt ggtggcccga aatccgtgtc gaatgccttg
     7501 cgcgagaagc ggttccaggt cacgggcatt cgtcgtcaac gtgaccgggt ggccttgggc
     7561 ggccagccac tccccgaccg tgcacaggtc ggcggcgtcc gcgcggtcga cggccaccac
     7621 cagcgactcg cgcgcggtga tcaccgattc gtcgagaaag ccgcggtgcc acagtgccag
     7681 cgactccagc ccgatctgca gcagccgctc ctcgccgggc cagccctcgc tatgcggggc
     7741 cagcattccg ccggccaccc aggaggcgcc cgggtcggcg ggattgcggt gcacccgcac
     7801 tgtgcggccc gacagcgcga gccgccgggc gaccgacaga ccgatgacgc cgccgccgat
     7861 caccgcgatg gactcgttgg acatgacggt ttctaggcta gtccgcgtgc gcgaagaccg
     7921 gctggcagaa gcccggctgt acctgtgcac cgacgcccgc cgggaacgcg gcgacctcgc
     7981 cgagttcgcc gacgcggcgc tggccggcgg cgtcgacatc atccagttgc gcgacaaggg
     8041 ctcggtcggc gagcagcggt tcgggccgct ggaggcgcgc gacgaattgg ccgcgctcga
     8101 ggtgctcgcc aacgcggccc gccgtcacgg cgcgttactg gccgtcaacg accgcgccga
     8161 catcgcgaag gcggccgggg ccgacgtgct gcacctgggt caggacgacc tgcccctgca
     8221 ggtggcccgg gagatcgccc cgcaaacgct gatcggccgc tccacccacg acaccgtgca
     8281 ggctcgcacc gcgctgaccg aggacgtcga ctatttctgc gtcggtccgt gctggccgac
     8341 accgacgaag ccgggtcgcc cggctcccgg tctggacctg gtccgcgccg tcgcgtcgat
     8401 gcgtaccgac aagccgtggt tcgcgatcgg cggcatcgac ctgcagcggc tgcctcaggt
     8461 gctcgacgcc ggcgcacgca gggtggtcgt cgtgcgggcg atcacggcag ccgatgaccc
     8521 ggcacaggcg gcgcgagatc tcagcgcggc acttgcagca gcggggtgac ccgactcagc
     8581 tcgagaagcg tgtgccagct ggccgcgaag ccaggatgca gtggcaggtc gccgacctct
     8641 tcctcgacca cccagcgcag ctcgacgctt tcgaagttcg gcatggtgtc cagcggatgc
     8701 ggcgcgtcgg cgaccaccgt cgtgtaagtc cagtggctgc cgctcgccgt ggccgtcacc
     8761 acggccgccc ggacgacgac gtgctcggcc gccagccccg cttcctctgc ggcctcgcgg
     8821 accgcggtct cctcggcggt ttcgtggctg tcgcgggcgc caccgggcaa cgcccaggtg
     8881 ccaccctgat ggctccacac tgcgcggtgc tgcagcagca ccacacggcc gccgtcagct
     8941 cgaggtgccc gcagcagtaa accggcggct ccgtgccggc cccaaaacat ggcaccggag
     9001 tccgacatca cccagccgtc gccgtcgccc cgcacggatt caggataagg aaaggaggag
     9061 caacccgagt cctgctggaa tctcttagaa tttgctcata cattgggcgc gaacgtgagc
     9121 atgaagggca cagaccagtg accgtagagc tggcgcatcc gtcgacggag ccggccccgt
     9181 cgcaggcagc tgtcgacccg gcccatcccc gctggtggtt ctggaccacg acgcccggtc
     9241 gcattctggt cagcggtgtc gtgctgatcg cgttggtgac gctcagcgcg ttcggcaccg
     9301 cgagcacgat cgaccgccgc caggaggcgc tgactacggt gctcaaccac accgagccgt
     9361 tggcgttcgc cgccgggcag ctgtacacca cgctgtcggt ggccgacgct gcggcggcga
     9421 ccgcgttcat cgcgggcgcc gagccgcgcg aggtgcgcca gcgttacgag caggccatca
     9481 ccgacgccgc ggtggcgctg acccaggcgt ccagcggcct gaccgaccag ccgatgctcg
     9541 agttgctggg ccggatcaac gcgcggctgg cggtctacac cgggttgatc gaaaccgcac
     9601 ggaccaacaa ccgggccggt aaccccgtcg gatcttcgta cctgtccgag gcatcgtcgc
     9661 tgatgcagca gcagatcctg cccgacgcgc aacggctgta cgacgagacg tcggcgcggg
     9721 tggacgccga gaccacggca tcgacgaaca tcccgacgct gctgatcgtg ttggtcgcgg
     9781 cgacgctgct attcggcgcc tactccaacc gctggctggc gcaaaaaaca cagcgacggc
     9841 tgaacttcgg cttcgtggcc ggcgggctgg cggtgctgat tatggtgatg tgggtgggaa
     9901 cggtgctggc gatttccacc acggacagcc gcagcgccaa gggcactgcc gccgattcac
     9961 tcaagaccat cacgaatctg gcgatcatgg cccagcaggc ccgcgcggac gagacgctgg
    10021 cgctgatcag gcgaggcgac gaggacatcc gcaagcagtc ctactaccag cggatcgaca
    10081 cgatgcatca gctgctcgac gagtacacca agcgcaagga cgcgatc
//
LOCUS       DASWBF010000149        11611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_476335,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000149 DASWBF010000000
VERSION     DASWBF010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476335"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VF874_05035"
     CDS             complement(<1..601)
                     /locus_tag="VF874_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9175475.1"
                     /translation="METGLAGRTVVVTGANANIGRGIALAFATEGANVVVVGRDEAQG
                     GRVCDQLLQRGAKDVLWQQCDVTNRPRVDAMAAAVHERFGSVDVLVNCVGGNVDFDAF
                     VDSDPKTWQQDIELNIMSTFHCTQAVLPDMIERRRGRIINIGSTSGIVGDPFLAVYSA
                     MKGAVHTFTKVLAKEVGRQGITVNAIAPYGTLPQSDQDIS"
     gene            complement(723..1496)
                     /locus_tag="VF874_05040"
     CDS             complement(723..1496)
                     /locus_tag="VF874_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX9175476.1"
                     /translation="MRGEAKCWLTDMDGVLVREEHALPGAADFLQRLVDKKRRFLVLT
                     NNSIFTPRDLAALLARSGLQVPEESIWTSALATAAFLDDQLHGGSAYVIGEAGLTTAL
                     HTVGYTLTDIDPDFVVLGETRTYSFEAITKAIRLILAGARFIATNPDVTGPSTEGPLP
                     ATGSVAALITKATGREPYFVGKPNPMMFRSALNRIEAHSESTVMVGDRMDTDVVAGIE
                     AGLETILVLSGSTSLDEVEKYPYRPSRILQSIADAIELV"
     gene            1547..2320
                     /locus_tag="VF874_05045"
     CDS             1547..2320
                     /locus_tag="VF874_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175477.1"
                     /translation="MHDLFRLDGKVAVVTGGGRGIGLMIARGLLQAGAKVYLSSRKSH
                     ELDAAVEELSPLGPVAAVPADLGTADGVNTLASALADREDAVHALFNNAGMAWGAPFD
                     DFPESGFDRILAVNIKGVFLLTRALLPMLKAASTPDDPARVVNTGSIDGIRPPERGRD
                     NFSYSASKAGVHMLTQHLAGELAPEILVNAIAPGLFQSRMTKAILAPGAEAVGAHLPL
                     GRIGQPDDIAGIAVFLASRASTYITAAVIPVDGGASVLR"
     gene            complement(2317..2445)
                     /locus_tag="VF874_05050"
     CDS             complement(2317..2445)
                     /locus_tag="VF874_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175478.1"
                     /translation="MDPNPDYDASDEIEYFFNWFAWGLRGISGPGGGYPPPAYPPV"
     gene            2576..3301
                     /locus_tag="VF874_05055"
     CDS             2576..3301
                     /locus_tag="VF874_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9175479.1"
                     /translation="MRPNGGSSPPDNGAATLDALRASGSSTELSRREELLAVATRLFA
                     ARGYHGTRINDVADVVGLNKATVYHYYASKSLILFDIYREAADRTVAAVHDEPSWSAR
                     EAIFHFTRRLLQNIANNIDGAAVYFQESPYITEWFTEEQVGYIRTAETTVYEHVRDVI
                     DRGIASGEFYECDSHVLALGYIGMTLGAYRWLRPTGRRSADEIAVEFSTALLRGLIRD
                     PAVREDSALGVRLGALPTSEPQP"
     gene            3301..3738
                     /locus_tag="VF874_05060"
     CDS             3301..3738
                     /locus_tag="VF874_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9175480.1"
                     /translation="MPVVSKTIEVAAAPETIMSIVADFEAYPQWNEEVKGCWVLARYD
                     DGRPSQLRVDVSIQGMDGTYICAVYYPSPAEIYTVLQQGDLFAKQQQNFSVVGMGATS
                     LLTVDLDVETKMAIPQMMVKKVVNDALDYLADNLKKRAEQLSA"
     gene            complement(3742..4238)
                     /locus_tag="VF874_05065"
                     /pseudo
     CDS             complement(3742..4238)
                     /locus_tag="VF874_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739275.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            4252..5662
                     /gene="fadD5"
                     /locus_tag="VF874_05070"
                     /pseudo
     CDS             4252..5662
                     /gene="fadD5"
                     /locus_tag="VF874_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242654.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD5"
     gene            5875..6675
                     /locus_tag="VF874_05075"
     CDS             5875..6675
                     /locus_tag="VF874_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9175481.1"
                     /translation="MTASTAGLSGYLRERARPPLEAVGGFFQMCVLTARATTKWPFQF
                     REFVIQGWFLMRVTFLPTVALAVPDTVLIIFTLNLLLVEFGAADVSGAGAGLAAVTQL
                     GPIVTVLVVAGAGSTAICADLGARTIREEIDAMEVLGIDPIHRLVVPRVVASTFVAVL
                     LNGAVIIIGLVGGFIFGVYFQNVSAGAYLSTLTLVTGLPEVVISVVKALTFGLIAGLV
                     GCYRGLTVSGGSKGLGTAVNETLVLSVVALFAVNVVLTTIGVRYGTGR"
     gene            6677..7546
                     /locus_tag="VF874_05080"
     CDS             6677..7546
                     /locus_tag="VF874_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9175482.1"
                     /translation="MTSSTVLRSRFPGTYSRVTKWGGAPGRFLDNTGHLAWFIVTAVG
                     QIPNALRHYRRAMLRLIAEIGMGTGAMAVVGGTVAIVGFVTLSGGSLIAIQGYATLGN
                     IGVEAFTGFVAALVNVRVVAPLVAGQALAATVGAGATAELGAMRISEEIDALEVMGIK
                     SICYLVSTRILAGLVVIIPLYAMAIILSFLSAQVTTTLFYGQSTGTYDHYFRTFLRPD
                     DVFWSFVQAVIIAIIVMINHCYFGYNASGGPVGVGEAVGRSMRASLVWIVLVVLFASL
                     ALYGTNPNFNLTV"
     gene            7549..8757
                     /locus_tag="VF874_05085"
     CDS             7549..8757
                     /locus_tag="VF874_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9175483.1"
                     /translation="MTAPLNSSRTPPYKLGGIIFVLLCVLIVVLVLLQFRGTFEPKTE
                     LTLMSDRAGLVMDPGSKVTFNGVVIGRVTEVTQVNVGDEPRAKLALNVKPQYIDLIPK
                     NVDAKIQATTVFGNKYVSFSSPKNPTGRIASGDVIEASSVTTEFNTLFETLVSIAEKV
                     DPVKLNETLSAAAQALSGLGDRFGQSIIHGNEILSDLNPQMPQIRYDNQRLADLAEVY
                     ADASPDLWAFLRNAVVTARTINEQQSNLDAALTASVGFGNTGADVFERGGPYLTRGAE
                     DLIPSSQLLDEYSPELFCTIRNFHDVAPKIADALGGNGYSLRTHSEILGAGNPYVYPD
                     NLPRVNAKGGPEGKPGCWQEVNRDLWPAPYLVMDTGASLAPYNHFELGQPLAIEYVWG
                     RQIGENTINP"
     gene            8754..9785
                     /locus_tag="VF874_05090"
     CDS             8754..9785
                     /locus_tag="VF874_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9175484.1"
                     /translation="MKITGTAIKLGAFSFVLLLFTAVIIVVFGQMRFDRTNGYSAIFS
                     NASGLRNGQFVRASGVEVGKVSKVELTDRGQRARVEFQVDRSLPLFEGTTASIKYLNL
                     IGDRYLDLERGDSDKRLPVGGTIPLERTKPALDLDALVGSFRPLFRALNADKVNNISQ
                     SIITVFQGQGGTINDILDQTAQLTSTLADRDQAIGEVIKNLNTVLGTAVRHQQDLDDT
                     IKNFEVLITGLKNRADPIADSTAEISNAAGTIAGILSDNRALLHDTLGHLETIQEPLI
                     DQKDQLNDVLVRFPAALKIIGRAGGVYGDFFNFYACDISLKLNGLQPGGPVRTVKIWS
                     QPTGRCTPQ"
     gene            9782..11338
                     /locus_tag="VF874_05095"
     CDS             9782..11338
                     /locus_tag="VF874_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9175485.1"
                     /translation="MRSLEGSNRVRTGLMGIVVVLIVVVVGQSFVSIPQLFAQPTYYA
                     QFKDAGGIKPGDKVLITGVEVGLVRSLAIEGDRIRIGFTLGGTQIGKDSRAAIRTDTI
                     LGRRNIQIEPRGSDLLRANNVLPEGQTSEPYQIYDAVDTFTRAAQDWDIDTVKQSLNV
                     LTETIDQTYPHLSDALKGVERFSDTIGKRDQQIDELLANANKIAGVLGNRSQQINTLL
                     VNAKTLLAAVNERGQAISYLLERVSAFSEQVKAFIDENPNLNHVLEQLRTISDILVQR
                     KFDLADSLTTLSKFTASLGEAIASGPYFKVMLVNLLPYWILQPFVDAAFKKRGIDPEN
                     FWRGAGLPAFRWPDPNGTRFPNGAPPPAPTPLEGTPDFPGPAVVAGSPCSYTPAADTI
                     PTAADPLPCSHLGVGPFGNNPYGPNYTGPNVVTSPPNPNGPGYTPGVTSAGVPGEVPP
                     ELQGVPVPIAPGPPGARTEPLGPVSGPYPAPAVPPADLPGPPPPPGPGPQVPDYPPPG
                     VSTPADIGGR"
     gene            11342..>11611
                     /locus_tag="VF874_05100"
     CDS             11342..>11611
                     /locus_tag="VF874_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9175486.1"
                     /translation="MSTIFNIRNLRLPNVSRAGLLIGAVVVVLAIVAAVVGAQVYKKL
                     TTNTVVAYFQQALALYPGDKVRIMGVKVGTIDKIEPAGDKMKITFH"
BASE COUNT         1979 a         3808 c         3748 g         2076 t
ORIGIN      
        1 cgctaatgtc ctggtcgctc tgcggaagtg tcccataagg cgcgatggcg ttgaccgtga
       61 taccttgtct gccaacctct ttggccaaga ccttggtaaa cgtgtgcact gcacccttca
      121 tcgccgagta gacggcgagg aaggggtcgc cgacgatccc ggacgtcgat ccaatattga
      181 tgatcctgcc gcgtcggcgc tcgatcatgt ccggcagcac agcctgcgtg cagtggaagg
      241 tgctcatgat gttgagctcg atgtcctgtt gccaggtctt cgggtctgag tcgacgaacg
      301 cgtcgaaatc gacatttccg ccaacgcagt tgacgagcac atcgacgcta ccgaacctct
      361 catggaccgc cgccgccatg gcgtcaaccc gcgggcggtt tgtcacgtcg cattgttgcc
      421 acagcacgtc tttggcaccc cgttgcagca gttgatcgca tacccgtcca ccttgtgcct
      481 cgtcgcggcc gacgactacg acgttcgcac cttccgtcgc gaaggccaag gcgatccccc
      541 gaccaatgtt ggcgttcgcc cctgtgacga cgacggtgcg gcccgcgagt cctgtctcca
      601 cgcgtcctct gtccatctgt gccgcagcgg cggcacccct tgtcgaaggc ctgattagag
      661 atcctgagcg tacccgggcg gtagttccta cctgggcaat ttcggaacgg atggatacct
      721 ggtcacacca gctctatcgc gtcggcgatc gactgcagga tccgacttgg ccgatacggg
      781 tatttctcca cctcgtccag cgacgtcgaa ccgctgagca caaggatcgt ctccagcccg
      841 gcttcgatgc ccgcgacaac gtcggtgtcc atccgatcgc ccaccatcac ggtgctctcc
      901 gagtgcgcct cgatgcgatt gagcgcgctg cggaacatca tcgggttcgg cttgccgacg
      961 aaatagggct cgcggccagt ggccttggtg atcagcgcgg ccaccgaccc ggtcgcgggc
     1021 agcggtccct cggtggacgg cccggtgacg tcggggttgg tcgcgatgaa ccgcgcgccg
     1081 gccagaatca gccggatcgc tttcgtgatg gcctcgaacg agtaggtccg cgtctcgccg
     1141 agcacgacga agtcggggtc gatgtcggtg agcgtgtagc cgacggtgtg cagcgcggtc
     1201 gtcagccccg cctcaccgat cacgtaggcg gagccgccgt gcaactggtc gtcgaggaag
     1261 gccgcggtcg ccagcgcgga cgtccagatc gactcctcgg gcacctgaag cccggaccgc
     1321 gccagcagcg cggccaagtc gcgcggcgtg aagatcgaat tgttggtcag cacaaggaat
     1381 ctgcgcttct tgtcgacgag ccgctgcaag aaatcggctg cgcccggcag cgcgtgctct
     1441 tcgcgcacca gcacgccgtc catgtccgtc agccagcact tggcttctcc acgcaccgga
     1501 ccagtctctc aacccgacaa tcgcgggccc cagactggtg gccatgattc acgacttgtt
     1561 tcgactcgac ggaaaggtcg ccgtcgtcac cggcggcggc cgagggatcg gcctgatgat
     1621 cgcgcgcggt ctgttgcagg ccggcgccaa ggtgtacttg tccagccgca agtcgcacga
     1681 gctcgacgcg gccgtcgagg agttgtcacc gctcgggccg gtggcagccg tcccggccga
     1741 tctgggcacc gccgacgggg tgaacacgct ggcgtcggcc ctggccgacc gtgaggacgc
     1801 ggtccacgcc ctgttcaaca acgcgggtat ggcgtggggc gcgccgttcg acgactttcc
     1861 ggagtcgggc ttcgacagga tcctcgcggt caacatcaag ggcgtgttcc tgctgacccg
     1921 ggcgttgctc ccgatgttga aggcggcctc gactcccgac gaccctgcgc gggtggtcaa
     1981 caccggcagc atcgacggca tccgcccacc cgagcgcggc agagacaact tctcctacag
     2041 cgccagcaag gcgggtgtgc acatgctcac ccaacatctt gcgggcgagc tggcgccgga
     2101 gatcctcgtc aacgcgatcg cgccgggcct gttccaatcg cggatgacga aggccatcct
     2161 cgccccgggc gccgaggcgg tgggcgctca cctgccgttg gggcgcatcg gccaacccga
     2221 cgacatcgcc ggtatcgcgg tgtttttggc cagccgcgcc agcacctaca tcaccgccgc
     2281 ggtgatcccg gtcgacggtg gggcgagtgt gctgcgttag acggggggat acgccggcgg
     2341 ggggtagccg ccgccggggc ccgaaatacc ccgcaggccc cacgcgaacc agttgaagaa
     2401 gtactcgatc tcgtcgctgg cgtcgtagtc cggattcggg tccacgtgag taccccctcg
     2461 gtgtcgggct tcggcgacga gtctatgccc cgccaccggc tggcgcgaag aactctctgg
     2521 caatccaacc ggttgattgg ctccagcgtc gtcgcgagaa atatagtgag tcgccatgcg
     2581 accaaatgga ggctcatcgc caccagataa cggtgccgcg acgcttgatg ctcttcgcgc
     2641 aagcggctca tcgaccgaac tctcccgccg agaggagctg ctggccgtcg cgacgagact
     2701 gttcgcggcc cgcggctacc acggcacccg gatcaacgac gtcgccgacg tggtgggcct
     2761 gaacaaggcc acggtgtatc actactacgc cagcaagtcg ctgatcctgt tcgacatcta
     2821 ccgcgaggcg gccgacagga ccgtcgccgc agtgcacgac gagccgtcgt ggtcggcgcg
     2881 cgaggccatt tttcatttca cccggcggct gttgcagaac atcgcgaaca acatcgacgg
     2941 ggccgcggtg tacttccagg aaagcccgta catcaccgag tggttcaccg aggagcaggt
     3001 cggctacatt cgcaccgccg agacgacggt ctatgagcac gtgcgcgacg tgatcgaccg
     3061 cgggatcgcc agcggcgagt tctacgagtg cgactctcat gtgctggcgc tcggctacat
     3121 cggaatgacg ctgggcgctt accgctggct gcggcccact ggtcggcgca gcgccgacga
     3181 gatcgcggtc gaattcagta ccgcgctgct gcgcgggctg attcgcgatc ccgccgtccg
     3241 cgaggactcg gcgctgggcg tgcggctcgg ggcgctgccg acctcggagc cgcaaccgta
     3301 atgccggtgg tcagcaagac gatcgaggtc gcggccgccc cggagaccat catgtcgatc
     3361 gttgccgact tcgaggccta cccgcagtgg aacgaggagg tcaagggctg ctgggtgctg
     3421 gcccgctacg acgacggccg gcccagccag ctgcgggtgg acgtgtccat ccaaggcatg
     3481 gacggcacct acatctgcgc ggtctactac ccgagcccgg ccgagatcta caccgtgctg
     3541 cagcagggcg acctgttcgc caagcagcag cagaacttct cggtggtggg gatgggcgcg
     3601 acgtcgctgc tgaccgtcga cctggacgtc gaaaccaaga tggcgatacc gcagatgatg
     3661 gtgaagaagg tcgtcaacga cgcgctcgac tacctggccg acaacctgaa gaagcgcgcc
     3721 gagcagctga gtgcgtagcg ctcagctcca gatgtcggac ttctccagcc gcgcgatcag
     3781 ccggcgtgcg ccgtcgtcga actgccagcc ggtcagcgcg accacctcgg cggtgtcgtg
     3841 ccgacgcagc gcctccacca gcctccggtg gttgtcgacc gccaacgccc ccactccggg
     3901 tcctgggcgt agatcagcgg cggcatgtac ctggccacgt gcagcagaaa ccacgcgagg
     3961 ttgatgcgtc cggtcgcgtg gttgaacacc cggtggtacg cgaactcggc cgcagcgacc
     4021 gcgtcggggt tggcgtcggc gactgcgccg gccagggcgt cggtgcagcg cgccagttcg
     4081 tcgagctcct cgtcggtgat ccgctcggat gcggtggcgg cgagctctct ggcgatcgtg
     4141 gcctgcagcc agaagatgtc ctcgacgtcg cggcgcgaaa gcggtgcgac gacgtgaccc
     4201 cgatgcggct cgagcactac catgccttcg ccgcgcagag tgcgatgtgc ctcccgcccc
     4261 gagttcgtcg aatcgttcct ggcgatcaac aagcttgggg ccatcgcagt gccggtgaac
     4321 ttccggatga cggcgcccga gatcgcgttc ctggtcggcg actgcgacgc gcgcgtggtg
     4381 atcaccgagt cggtgctcgc gcgtgttgct accgcggtgc gtgacctcga cccgacgctg
     4441 gccaccgtcg tcgtcgccgg gtccgggtca gaagacgccg tgctcgacta cgaggctctg
     4501 ctggctgaag acgccaaacc gcacccactt gtcgacatcc ccaacgaatc gcccgcgctg
     4561 atcatgtata cctcgggcac cacgggccac cccaagggcg cggtgctgac ccacaccaac
     4621 ctcgtcggcc aggcgatgac cttcctgttc accaacggcg tcgatctcaa caacgacgtc
     4681 ggcttcatcg gcgtcccgct gttccacatc gccggcatcg gcaacatgat catcgggttg
     4741 ttgctggggt tgccgacggt gatctacccg ctgggcgcat tcgagcccgg cgtgcttctc
     4801 gacgtgctcg aagccgagaa ggtgacggcg atcttcttgg tgccggctca gtggcaggcg
     4861 gtctgcgccg cacagcaggc gcagccgcgt gaccttcggc tacgcgtgtt ttcctggggt
     4921 gcggcgccgg cccccgattc gctgctgcgc cagatgtcgg acaccttccc cgggacccag
     4981 atcctggccg cgttcgggca gacagagatg tcgccggtca cctgtatgtt gttgggcgac
     5041 gacgcaattc gcaaactcgg ctccgtcggc aaggtgatcc ccaccgtcgc cgcgcgggtg
     5101 gtggacgacg acatgaacga cgtcgccgtc ggcgaggtcg gcgagatcgt gtaccgggcg
     5161 ccgacgctca tggcccggct actggaacaa cccgcaggcc accgcggagg cctttgcccg
     5221 cggctggttc cactcgggcg atctggtgcg ccaggacgaa gagggctacg tctgggtggt
     5281 cgaccgcaag aaggacatga tcatttccgg cggcgagaac atctactgcg ccgaggtcga
     5341 gaacgttctg gcggcccatc cggcaatcat cgaagtggct gtgatcggcc gtccgcacga
     5401 gcggtggggc gaagttccga tcgccgttgt ggccgttgct gagtccggtc tcaccctcgc
     5461 tgatcttgat ctattcctca gcgagcggct cgcgcgttac aagcatccca aaggtctgga
     5521 aattgtcgac gcgcttccac gcaaccccgc tggcaaagtc ctgaaaactg aactgcggac
     5581 caggttcggt gcggcgaccg tcaacggccc cggccaaagc ggctctgcgg caacagtttc
     5641 tgccggggcg acggacggct gaacggggtg atcgtgtttg ctaacggttc gagagtcaat
     5701 cgtgcggatg cggtacgcgg ccgggttctc atcgggtaca gtcctgtggt ccgtcttact
     5761 accaaccggt agggagatga gtgtcacctg gtcggtactg gggcaaggag ggggcgtgcg
     5821 acacgatctg ccgttgcgcc gcgttgaacg cggctacgac gtgcgggtgc tccggtgacc
     5881 gcgtcgaccg ccgggttgtc cggttatttg cgcgaaaggg cccggccgcc attagaggct
     5941 gtcggtggct tcttccagat gtgtgtgctg acggccagag cgacgaccaa gtggccgttc
     6001 caattccgcg agttcgtcat tcagggctgg ttcctgatgc gggtgacttt tctgccgacc
     6061 gtcgccctgg ccgtgccgga caccgttctg atcatcttca cgcttaacct tttgctggtc
     6121 gagttcggcg cggcggatgt gtcgggagcc ggggccggat tggccgccgt cacccagctg
     6181 ggccccatcg tgactgtgct cgtggtcgcc ggagccgggt cgaccgccat ttgcgcggac
     6241 ctcggtgccc gcaccatccg cgaggagatc gacgcgatgg aggtgctcgg catcgacccg
     6301 attcaccgcc tggtggtccc ccgggtggtc gcgtcgacat tcgtcgcggt tctgctcaac
     6361 ggtgcggtga tcatcatcgg cctggtcgga ggtttcatct tcggcgtgta cttccagaat
     6421 gtttccgccg gcgcgtactt gtcgaccctc acccttgtca cgggcctgcc cgaagtggtg
     6481 atctcggtcg tcaaggccct gacgttcgga ctcatcgcgg gtttggtggg gtgttaccgc
     6541 gggttgaccg tgtccggtgg ctccaaaggg ttggggacgg ccgtcaacga aaccctggtg
     6601 ctatccgtcg ttgcgctgtt cgccgtcaac gtcgtgctga cgaccatcgg cgtgcggtat
     6661 ggaacggggc gttgaagtga cttcgtcgac cgttctccgc agccggttcc ccgggacgta
     6721 ctcgcgagtc accaagtggg gcggtgcccc cgggcggttt ctcgacaaca ccggtcacct
     6781 ggcgtggttc atcgtcaccg ccgtcggtca gatcccgaat gcgctgcgcc actatcggcg
     6841 cgcgatgttg cggctgatcg cagagatcgg catgggcacc ggagcgatgg ccgtggtggg
     6901 cggtaccgtc gcgatcgtcg gcttcgtgac actctcgggc gggtcgctga tcgccatcca
     6961 gggctacgcg acgctcggca acatcggtgt ggaggcgttc accggcttcg ttgctgcgct
     7021 ggtcaacgtg cgcgtcgtcg caccgctggt cgccggccag gcgcttgccg ccacggtcgg
     7081 cgcgggtgcc accgccgagc tcggcgcgat gcggatcagc gaggagatcg acgcgctcga
     7141 agtcatgggg atcaagtcga tctgctacct ggtgtcgacg cgcatcctgg ccggtcttgt
     7201 cgtcatcatc ccgctctacg cgatggcgat catcctgtcg ttcctgtcgg cgcaggtgac
     7261 cacgacgctg ttctacgggc agtcgaccgg cacctatgac cactacttcc gcaccttcct
     7321 gcgccctgac gacgtgttct ggtcgttcgt gcaggcagtg atcatcgcga tcatcgtcat
     7381 gatcaaccac tgctacttcg gctataacgc cagcggcgga ccggtcggtg tgggtgaggc
     7441 cgtcggccgg tcgatgcgcg cgtcgctggt ttggatcgtg ctcgttgttc tgttcgcctc
     7501 gttggcgctg tatggcacca acccgaactt caacctgacg gtgtagtcat gaccgcgccg
     7561 ttgaacagct cccgaacgcc accctacaaa ttgggtggaa tcattttcgt cctgctgtgc
     7621 gtgttgatcg tcgtcttggt tctgctgcag ttccgcggga cgttcgagcc caagaccgag
     7681 ctgacactga tgtccgatcg ggccggcctc gtgatggacc cgggttcgaa ggtcaccttc
     7741 aacggcgtgg tgatcggcag ggtcaccgag gtcacccaag tcaacgtcgg cgacgaaccg
     7801 agggcgaagc tggcgctgaa tgtcaagccg cagtacatcg acctgatccc gaagaacgtc
     7861 gatgccaaaa tccaggcgac gacggtgttc ggcaacaagt atgtgtcgtt ctcgtcgccg
     7921 aaaaacccga ccgggcggat cgcctccggt gacgtcattg aggcgtcttc ggtgaccacc
     7981 gaattcaaca cgttgttcga gacgttggtg tcgatcgcgg agaaggtcga tccggtcaag
     8041 ctcaacgaga cgctttcggc tgcggcgcag gcgttgagcg ggctgggcga tcggttcgga
     8101 cagtcgatca tccatggcaa cgagatcctc tccgatctga atccgcagat gccgcagatc
     8161 cgctatgaca atcagcggtt ggcggacctg gcggaggtgt acgccgacgc gtcaccggac
     8221 ctgtgggctt tcctgcgcaa cgcagtggtc accgcccgca ccatcaacga gcagcagtcc
     8281 aatctcgatg cggcgttgac ggcgtctgtc ggcttcggca acaccggcgc cgacgtcttc
     8341 gagcgcggcg gcccgtatct gacgcgcggc gccgaagacc tcattccgtc gtcccagctg
     8401 ctcgacgaat acagccccga gctgttctgc accatccgca acttccacga cgtcgcaccg
     8461 aagatcgccg atgcgcttgg cggcaacggt tattcgctga ggacgcactc ggagatcctc
     8521 ggcgccggca acccgtacgt ctatccggac aacctgccgc gggtgaacgc gaagggcggc
     8581 ccggagggca agcctggctg ctggcaggaa gtcaaccgtg atctgtggcc ggcgccatac
     8641 ctggtgatgg acaccggtgc ctcgcttgcg ccgtacaacc acttcgagct cggccagccg
     8701 ctcgcgatcg agtacgtctg gggccgccag atcggggaga acacgatcaa cccatgaaaa
     8761 tcaccggtac cgccatcaag ctcggagcat tctcgttcgt gctcctgctg ttcaccgcag
     8821 tcatcatcgt ggtgttcggc cagatgcggt tcgaccgcac caacggttat tcggcgatat
     8881 tcagcaacgc gagtggactg cgcaacggtc agttcgtccg cgcgtcggga gtggaggtcg
     8941 gcaaggtctc caaggtggag ctcaccgacc gtggccagcg ggccagggtg gagttccagg
     9001 tcgaccggtc cttgccgctg ttcgagggga ccaccgcgtc catcaaatat ctgaacctga
     9061 tcggcgaccg ctacctcgac ttggagaggg gcgacagcga caagcggctg cccgtcggcg
     9121 gcacgattcc tctcgagcgg accaaaccgg cgcttgacct cgacgcgctg gtcggtagct
     9181 tccggccgct gttccgtgcg ctcaatgcgg acaaggtcaa caacatctcg cagtcgatca
     9241 tcacggtgtt ccagggccag ggcggcacga tcaacgacat cctggatcag acggcgcagc
     9301 tcacgtcgac gctggcagac cgcgaccagg cgatcggcga ggtgatcaag aacctcaaca
     9361 ctgtgctggg caccgccgtc cggcatcaac aggatctcga tgacacgatc aagaacttcg
     9421 aggtgctgat caccggactg aaaaaccgtg ccgacccgat cgcggactcc accgcggaga
     9481 tcagcaacgc ggcgggcacc atcgcgggca tcttgtccga caatcgcgcc ctactgcacg
     9541 acaccctcgg tcacctggaa accatccagg agccgctcat cgaccagaag gaccagctca
     9601 acgacgtcct ggtcaggttc ccggccgcac tgaagatcat cgggcgggcc ggcggcgtct
     9661 acggtgactt cttcaacttc tacgcgtgcg acatctcgct gaagttgaac ggactgcaac
     9721 cgggcggtcc ggtgcgcacg gtcaagatct ggtcgcagcc cacaggcagg tgcacgccgc
     9781 aatgaggtcg ttggaaggct caaatcgagt tcgcaccggg ctgatgggca tcgtggtcgt
     9841 gctgatcgtc gtcgtcgtcg ggcagagctt cgtcagcatt ccgcagttgt ttgcgcagcc
     9901 gacctactac gcgcagttca aggacgccgg gggtatcaaa cctggcgaca aggtgctgat
     9961 caccggtgtc gaggtcggct tggtgcgctc gttggcgatc gagggcgacc gcatccgcat
    10021 cggcttcaca ctcggcggca cgcagatcgg caaggacagc cgggcagcga tccgcaccga
    10081 cacgatcctc ggccgcagga acatccagat tgagccccgc ggctcagact tgttgcgcgc
    10141 caacaatgtg ctgcccgagg gacagaccag cgagccgtac cagatttacg acgctgtcga
    10201 caccttcacc agggcagccc aagactggga catcgacacc gtcaagcagt cactcaatgt
    10261 gctcacggaa accatcgacc agacgtaccc gcatctgagc gacgcgctca agggcgtcga
    10321 gcggttctcc gacacgatcg gcaagcgtga ccagcagatc gacgaactgc tggccaacgc
    10381 gaacaagatc gccggagtgc tcggcaaccg cagccagcag atcaacaccc tgctggtgaa
    10441 cgccaagacg ctgctggcgg ccgtcaacga acgtggccag gcgatttcct acctgctcga
    10501 gcgcgtgtcg gcgttctccg agcaggtgaa ggcgttcatc gacgagaacc ccaacctcaa
    10561 ccacgtgctc gaacagctgc ggacgatcag cgacatcctg gtgcagcgca agttcgatct
    10621 cgcggactcg ctcaccacgc tgagcaagtt caccgcatcg ctcggcgagg cgatcgcctc
    10681 cggcccgtac ttcaaggtga tgctggtcaa cctgctgccg tactggattc ttcagccgtt
    10741 cgtggacgcg gcgttcaaga agcgcggcat cgaccccgag aacttctggc gcggtgccgg
    10801 tctgcccgcg ttccgctggc cggatcccaa cggcacccgg ttccccaacg gcgcaccgcc
    10861 gccggcgccg accccgctgg agggcacacc cgacttcccg gggcccgcgg tggtcgccgg
    10921 ctccccgtgc tcgtacacac cggccgccga cacgataccg acagcggcag atccgctgcc
    10981 gtgctcgcat ctgggcgtgg gaccgttcgg caataacccg tacgggccca actacacggg
    11041 gccgaacgtg gtgacgtcgc cacccaaccc gaacggtccc ggatacacgc caggcgtcac
    11101 gagcgccggt gtgccggggg aggttccgcc ggagttgcag ggagtaccgg tgccgatcgc
    11161 gcccgggccc ccgggtgcac gcaccgaacc gcttggcccg gtttcgggtc cgtacccagc
    11221 gccggccgtg ccaccggctg acctgccggg gccgccgcca cctcccgggc ccggcccgca
    11281 ggtgccggac tacccgccgc caggtgtgtc cacgcccgca gacattggag gccgctgacc
    11341 gatgtcgacg atcttcaaca tccgaaacct aaggctgccc aacgtttctc gggctggcct
    11401 cctcatcggt gccgtggtcg tggtgctggc catcgtcgcc gcagtggtcg gcgcgcaggt
    11461 ttacaagaag ctgaccacca acaccgtcgt cgcgtatttc cagcaggcgc ttgcgctgta
    11521 tcccggcgac aaggtgcgga tcatgggcgt caaggtcggc acgatcgaca agatcgagcc
    11581 cgccggcgac aagatgaaaa tcacgttcca c
//
LOCUS       DASWBF010000150         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_996156,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000150 DASWBF010000000
VERSION     DASWBF010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996156"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2035
                     /gene="carB"
                     /locus_tag="VF874_05105"
     CDS             <1..>2035
                     /gene="carB"
                     /locus_tag="VF874_05105"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX9175487.1"
                     /translation="DELLDRLKTPTDGRLYDIELALRLGAGVEEVAAASGVDPWFVEQ
                     IARVVALRTELIDAPVLDADLLRRAKHSGLSDRQIAALRPELAGEVGVRALRQRLDIH
                     PVFKTVDTCAAEFEAKTPYHYSSYELDPAAETEVAPQTDKPKVLILGSGPNRIGQGIE
                     FDYSCVHAATTLSDAGFETVMVNCNPETVSTDYDTADRLYFEPLTFEDVLEVYYAESQ
                     SGAGGPGVAGAIVQLGGQTPLGLAQRLEDAGVPIVGTSPRAINSAEDRGAFGEVLSDA
                     GLPAPRFGTATTFEHARKIAAEIGYPVLVRPSYVLGGRGMEIVYDEQTLHGYITRATE
                     LSPEHPVLVDRFLEDAIEIDVDALCDGSEVYLGGIMEHIEEAGIHSGDSACALPPVTL
                     GRSDIDNVRRSTEAIAHGIGVVGLLNVQYALKDDVLYVLEANPRASRTVPFVSKATAV
                     PLAKACARIMLGASISRLRDEGMLARTGDGATVAAYAPIAVKEAVLPFHRFRKADGGQ
                     VDSLLGPEMKSTGEVMGIDRDFGTAFAKSQTAAYGSLPAEGTVFVSIANRDKRSLVFP
                     VKRLADLGFRVLATAGTAEMLRRNGIPCDEVRKHFEDPSPDRPALSAVDAIKAGEVDM
                     VINTPFGNSGPRIDGYEIRSAAVAMNIPCVTTVQGASAAVQGIEAGIRGDIGVM"
BASE COUNT          354 a          688 c          690 g          303 t
ORIGIN      
        1 gacgagctgc tggaccggct caagaccccg accgacgggc ggctctacga catcgagctg
       61 gcgctgcggc tgggcgcagg cgtcgaagag gtcgcggcgg cctccggcgt cgacccgtgg
      121 ttcgtcgagc agatcgcgcg cgtcgttgca ttgcgaaccg agctgatcga cgctccggtg
      181 ctcgacgccg acctgctgcg ccgcgccaag cacagcggcc tgtccgatcg ccagatcgcg
      241 gcgttgcgac cggaattggc cggtgaggtg ggggtgcggg cgctaaggca gcgactcgac
      301 atccaccccg tgttcaagac cgtcgacacc tgcgccgcag aattcgaagc caagacgcct
      361 taccactaca gcagctacga actcgacccc gccgccgaaa cagaagtcgc gccgcagacc
      421 gacaagccga aggtgttgat cctcggctcg ggccccaacc ggatcggcca aggcatcgaa
      481 ttcgactaca gctgcgtgca cgcggcgacc acgctgtccg acgccggctt cgagacggtg
      541 atggtgaact gcaacccgga gacggtctcc accgactacg acaccgccga ccgcctttac
      601 ttcgagccgc tgacattcga agacgtgctc gaggtgtact acgccgaatc gcagtccggc
      661 gcaggcggtc ccggtgtggc cggggcgatc gtgcagctcg gcgggcagac gccgctggga
      721 ctggcccagc gcctggagga cgccggcgtg ccgatcgtcg ggaccagccc ccgggcgatc
      781 aactcggccg aggaccgcgg cgcgttcggc gaggtactca gcgacgccgg actgcccgca
      841 ccccgattcg gcaccgcgac aacctttgag catgcccgca agatcgctgc cgagatcggc
      901 taccccgtcc tggtccggcc gtcgtacgtg ctgggtggcc gcggcatgga gatcgtctac
      961 gacgaacaaa cgttgcacgg ctacatcacc cgcgccaccg agctgtcccc cgagcacccg
     1021 gtcctcgtgg acaggttcct cgaggacgcc atcgagatcg acgtcgacgc gctgtgcgac
     1081 ggctccgagg tgtacctggg cggcatcatg gaacatatcg aggaggccgg tatccattcc
     1141 ggcgactctg cctgcgcgct tcccccagtg acattgggcc gcagcgacat tgacaatgtc
     1201 cgccgctcca ccgaggcgat cgcgcacggg atcggcgtcg tcgggttgct caacgtgcag
     1261 tacgcgttga aggacgacgt gctctacgtg ctggaggcca acccgcgggc cagccgaacc
     1321 gtgccgttcg tgtcgaaggc cacggcggtg ccgctggcga aggcctgcgc gcgaatcatg
     1381 ctgggcgcct ccatctcccg gttgcgtgac gaaggaatgc tggcccgcac gggtgacggc
     1441 gcgacggtgg ccgcgtacgc cccgatcgcg gtcaaggaag ccgtgctgcc gttccaccgg
     1501 ttccgcaagg ccgatggcgg ccaggtcgac tcgctgctgg ggccggagat gaagtccacc
     1561 ggcgaggtga tgggcatcga ccgcgacttc ggcaccgcat tcgccaagag ccagaccgcc
     1621 gcctacgggt cgctcccggc cgagggaacg gtgttcgtgt cgatagccaa ccgcgacaag
     1681 cgatcgctgg tgttccccgt gaagcggcta gcagacctgg gattccgcgt gctggcaacc
     1741 gcgggcacgg cggagatgtt gcgccgcaac ggaattccat gcgacgaggt acgcaagcat
     1801 ttcgaagacc cgagccccga ccggcccgcg ctgtcggcgg tggacgccat caaggccggc
     1861 gaggtcgaca tggtcatcaa tacgccgttc ggcaattccg ggccacgcat cgacggctac
     1921 gaaatccggt ccgcggcggt ggcgatgaac atcccctgcg tgacgacggt acagggcgca
     1981 tcggctgcgg tccagggtat cgaggccgga atccgcggcg acatcggcgt gatgt
//
LOCUS       DASWBF010000151         8127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_601026,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000151 DASWBF010000000
VERSION     DASWBF010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601026"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VF874_05110"
     CDS             complement(<1..663)
                     /locus_tag="VF874_05110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX9175488.1"
                     /translation="MSEPPTEARRLLFIGDISAVANRGTPEVDDRLRALMASADLVVG
                     NCESPVVERGRRLLGTRHTMSPRFVASALEAAGIEPQRLVLSLANNHMLDQGVNGYAE
                     TREHLSRLGIATIGGSDDGLVRTIELDGLEVGFAAFTKWRNASKAAFAGRVTDLEDLL
                     DDDAAPLHHSRADLLCVVAHWDYEFRHCPRPDTRALAHDLAESGAGLIVGHHAHVLQP
                     VER"
     gene            860..2050
                     /locus_tag="VF874_05115"
     CDS             860..2050
                     /locus_tag="VF874_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX9175489.1"
                     /translation="MAPDVFSPAKLGPITLRNRVIKAATFEAATPEALVTDDLIEYHR
                     LPAAGGVAMTTVAYCAVSPGGRTERRQIWMRPDAVPGLRRLTEAVHAEGAAISAQIGH
                     AGPVADPRSNKAKALAPVRFFNPAAMRFARKASRDDIRDVMAAHADAARLAIDAGFDA
                     VEIHLGHGYFCSSFLSPLINRRDDEFGGSLENRAKVARGVVQSVRRAVGDQIAVTAKL
                     NMADGIRGGIRVDEALTTAKWLEDDGRLDALELTAGSSLVNPMYLFHGDVPLQEFANR
                     FKPPMRWGVRMMGPKFLREYPYRDAYLLDLARKFRAELSMPLILLGGITNRETMDLAM
                     AEGFEFVAMARALLAEPDLVNRIAADGVSVRSACTHCNLCMPTAYRVTADSRTRCVLT
                     GAPE"
     gene            2112..4730
                     /locus_tag="VF874_05120"
     CDS             2112..4730
                     /locus_tag="VF874_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9175490.1"
                     /translation="MSRPSPPALTVRTDGSQRTFAPGHDVVIGRDLRADVRVAHPLVS
                     RAHVVLRFDNGRWTAIDNGSLNGMFVDHRRVPTVDIRDGQTINIGNPDGPRLTFEVGR
                     HQGAVGRPPQTTSVPLSAAAAADGTWKGPQQSMSRAPAAYPPSAPQVRPPSGPQVRPP
                     SGPQSRPPSGPQPVSRPPSRPAQPVSRPPSAPPPQQNYQATQIGPAARPTEAAGQNLA
                     TSMIRILRGGGPAAPPVGAIRIGRATDNDIVIADVLASRHHATLMPTPSGTEIRDERS
                     INGTFVNGSRVDRALLGDGDVVTIGNVDLVFQGGTLVRRTETEADTRTGGLEVHGVTW
                     TIEGNKTLLHNISISARPGTLTAVIGPSGAGKSTFAKLVAGYTQPTTGTVAFEGHNIH
                     AEYASLRSRIGMVPQDDVVHGQLTVNQALMYAAELRLPPDTTKADRAQVVAQVLEELE
                     MTRHADTRVDKLSGGQRKRASVALELLTGPSLLILDEPTSGLDPALDRQVMTMLRDLA
                     DAGRVVLVVTHSLTYLDVCDQVLLLAPGGKTAFFGPPSEIGASMGTTNWADIFSTVAS
                     DPDAANRRFLDQHGPPPPPPPAEQPKDLGSPTRTSLWRQFSTIARRQVRLIVSDRGYF
                     IFLMMLPFIMGVLATTVPGEAGFGSPSPTSESPNEPGTILVLLNVGAIFMGTALTIRT
                     LIGERAIFLREQAVGLSTTAYIFAKMCVFTVFAVLQATIAVAITVWGKGAPTQGAVML
                     KNPTLELWFDVAFTCVAAAMVGLALSALARSNEQIMPLLVVAIMSQLVFSGGMIPVTG
                     RLVLDQLSWLTPARWGFAASASTADLLKLVPGPVTPKDSHWKHTPSAWMFDVGMLFVI
                     CVGYATFVRWRIRLKS"
     gene            complement(4732..5553)
                     /locus_tag="VF874_05125"
     CDS             complement(4732..5553)
                     /locus_tag="VF874_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9175491.1"
                     /translation="MTHAWVPVAPCDESCVRDDPVRFLGRIVRIVRGCWRTFLIFAVL
                     PLMPFTAIPHPGQDRLVRHYSQLLLRCVGVRIAMPDNVIRDMPGALVVSPHISWADVL
                     VIWAVMPATFVAKADMIKWPGIGQMARALGAIPIDRTKLRPLPGVVAQVTARLKSGRT
                     VMTFPEGTSWCGLGYGPFRPAMFQAAIDADRPVLPLRLSYHHRDGRLSTVPAFVGEDT
                     LTRSVWRVVATRRTVVEIHVGALQMPGTDRRELARRCQEVVCGSVELQKEDELAA"
     gene            complement(5550..6344)
                     /locus_tag="VF874_05130"
     CDS             complement(5550..6344)
                     /locus_tag="VF874_05130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX9175492.1"
                     /translation="MSTASVLLASDSRDSEAPKYSLLLSTDPALIEAAQRLRYEVFTL
                     EPGFTLTAASDADCFDQYCDHLLIRDDSSGELVGCCRVLPPAGAVAAGGLYTATEFDV
                     HELDPLRASLVEMGRAVVRADHRNGAVVLLMWAGILAYLDHCGHNYVTGCVSVPIQGV
                     GLPGQQARGVRDFVLKRHRSAYTVQPYRPVSFDGIPLPMRVTVPPLMRGYLRLGTNVC
                     GEPAHDPDFGVADFMVLLDKREADIRYLNRLRSVAAASEMAEEIQQ"
     gene            complement(6559..7389)
                     /locus_tag="VF874_05135"
     CDS             complement(6559..7389)
                     /locus_tag="VF874_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX9175493.1"
                     /translation="MQFIANRQNVSGKAAAVKAQTQRTDGVEDTISRPGLLNRALGGL
                     VSWAEQLNWKYSKVGNRPVFTTAEFPWATEIEREWHLIRAELDRLLTRKDELPGFHEI
                     IGEVRAISTDRDWKTFLFVGFGAKSEEAARQCPQTWRIVQTIPQLQTAMFSILEPGKQ
                     LKPHRGVYNGVLRLHLGLIVPNSPPGTVAIRVDNEVCHWDEGKVLIFDDSYEHEVWNH
                     SEQTRVVLFVDFVKPLRFPASFINWLILHIAPFTPYIREGAKAQKEWEKGFFRKPRSA
                     "
     gene            7559..>8127
                     /locus_tag="VF874_05140"
     CDS             7559..>8127
                     /locus_tag="VF874_05140"
                     /inference="COORDINATES: protein motif:HMM:NF022198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HEX9175494.1"
                     /translation="MGVAIATISLIVGLVGCGKDSKTSTTSTTTTTTTTTTTTTTSAQ
                     ASGPHQTLGDYSKENNIQETLISRGTPGAPVVDLGLPDGWTAQPESNDAPYGAIVFDT
                     PSDPNDPPRFRAFFEKLTGNVDTDKLLAASPGEVLNLPGYEGGEGQRDTLANFPAYSV
                     GGEYTKDGAKRMAAQKAVVIQGSDGIYLLL"
BASE COUNT         1453 a         2697 c         2637 g         1340 t
ORIGIN      
        1 ccgctcgacc ggctgcagca catgggcgtg atgcccgacg atcagtccgg ccccgctctc
       61 ggcgaggtcg tgcgcgagcg cgcgcgtgtc ggggcgggga cagtgccgga actcgtaatc
      121 ccaatgcgcg acaacgcaaa gcaggtcggc gcgcgagtga tgcaacggcg ccgcgtcgtc
      181 gtcgagcagg tcctcgaggt cggtcacacg tcctgcaaag gctgccttac tcgcgttgcg
      241 ccatttggtg aaggcagcga agccgacctc aaggccgtcg agttcgatag ttcgcacgag
      301 cccgtcgtcg ctgccgccga tcgtcgcgat gccgagacgc gaaaggtgct ctctggtttc
      361 ggcgtaaccg ttcaccccct ggtcgagcat gtggttgttc gccagcgaca gcaccaaccg
      421 ctgcggctcg atgccggcag cctccagtgc cgaggcaacg aagcggggcg acatggtgtg
      481 gcgcgtgccg agcagtcgcc ggccccgctc gacgaccgga ctctcgcaat tgccgacgac
      541 cagatcggcc gaggccatca acgccctgag ccgatcgtcg acctcgggtg tgccgcggtt
      601 cgccactgcg gatatgtcgc cgatgaagag cagacgtctc gcttccgtcg gcggctccga
      661 catgagcgct gttttagccg gcagggagtt ctgcctgtcg ccgcgaagcg acggcctcag
      721 caggcgcggc agccacgaga gttggtaggc gagcggaaag ttggacacgg tttcgcgata
      781 gtcgcagaac taagcgagtc agtcagccgg tttgcttgcc cgaacggcag tcgaacacgc
      841 atgtggcagg ctccgggtta tggctcctga cgtgttctcc ccggccaagc tcgggccgat
      901 cacgctgcgc aatcgggtca tcaaggcagc cacgttcgag gcggccactc cagaggcgct
      961 ggtcaccgac gacctgatcg aataccaccg ccttcctgcc gccggcgggg tggcgatgac
     1021 gaccgtcgcg tactgcgccg tctcgcccgg cggacgcact gagcggcggc agatctggat
     1081 gcggcccgac gccgtgccag ggctgcgccg actgaccgag gcggtgcatg cagagggcgc
     1141 cgcgatcagc gcgcagatcg gccacgccgg gcccgtcgca gaccctcgct ccaacaaggc
     1201 caaggccctg gcgccggtcc ggttcttcaa cccggccgcg atgaggttcg ccaggaaggc
     1261 cagccgcgac gacatccgcg acgtgatggc cgcgcacgcc gacgcggcac ggttggcgat
     1321 cgacgccggg ttcgacgccg tggaaattca cttgggccac gggtacttct gcagctcctt
     1381 cctcagcccg ctgatcaacc gccgcgacga cgagttcggc gggtcgctgg aaaaccgcgc
     1441 gaaggtggcc cgcggcgtgg tgcagtcggt tcgtcgcgcg gtcggcgacc agatcgcggt
     1501 gacggcgaaa ctcaacatgg ccgacgggat tcgcggcggc atccgcgtcg acgaggcgct
     1561 gacgaccgcg aagtggcttg aagacgacgg caggctcgac gcgctcgagc taaccgcggg
     1621 cagctcgctg gtcaacccga tgtatctgtt ccacggcgac gttccgctgc aggagttcgc
     1681 gaacaggttc aagccgccga tgcgctgggg cgtgcggatg atgggcccga agttcctgcg
     1741 cgagtatccc taccgcgacg cgtatctgct cgacctggcc cgcaagttcc gcgcggagct
     1801 gtcgatgccg ttgatcctgc tcggcggcat cacgaaccgg gaaacgatgg accttgcgat
     1861 ggccgaggga ttcgagttcg tcgcgatggc gcgggcgctg ctcgccgagc cggatctggt
     1921 caaccggatc gcggccgacg gggtttcggt gcgctccgcc tgcacacact gcaacctgtg
     1981 catgcccacc gcctaccggg tcaccgccga cagccgaacc cgctgcgtgc tgaccggtgc
     2041 cccggaatag ctgtgagaag accggctgac ccgcctcgcg ctgcgggcaa atacctatag
     2101 agttactccc gatgagccga ccttcgccgc ccgcgctgac cgttcggaca gacggatcgc
     2161 aacgaacctt tgcgccgggc catgacgtgg tcatcgggcg tgacctcaga gccgacgtcc
     2221 gcgtcgcgca tccgttggtg tcgcgcgccc atgtggtgct gcgcttcgac aacggccgtt
     2281 ggacggcgat tgacaacggc agcctgaacg ggatgttcgt cgaccaccgg cgggtgccga
     2341 cggtggatat ccgcgacggg cagaccatca atatcggcaa cccggacggg ccgcgactga
     2401 cgttcgaggt cggacgccat cagggcgcgg tcgggcggcc gccgcagacg acctcagtgc
     2461 cgttgtccgc ggcggcggcg gcggatggca cgtggaaggg accgcagcag tcgatgtcgc
     2521 gcgctccggc ggcgtatccg ccgagcgcgc cgcaggtccg gccgccgagt ggaccgcaag
     2581 tccggccgcc cagcggccca cagagccggc cgccgagtgg gccgcagccg gtgtcacggc
     2641 cgccgtcgcg gcccgcccag ccggtgtccc gcccgccgtc ggcacccccg ccgcagcaga
     2701 actaccaggc gacacagatc gggcccgccg ctcggcccac cgaggccgcc ggtcagaacc
     2761 tcgcgacgag catgatccgg attctgcgcg gtggcggccc cgcggccccg ccagttgggg
     2821 cgatcaggat cggccgcgcc accgacaacg acatcgtgat cgccgacgtg ctggcgtcgc
     2881 ggcaccacgc gacgttgatg ccgaccccgt cgggcaccga gatccgggac gagcgaagca
     2941 tcaacggcac gttcgtgaac ggctctcggg tcgatcgggc gttgctgggt gacggcgatg
     3001 tggtgacgat cggcaatgtc gacctggtgt tccagggcgg cactctggtc cgccgtaccg
     3061 agaccgaagc cgatacccgc acaggcggtt tggaagtaca cggcgtcacg tggaccatcg
     3121 aaggcaacaa gacgctgctt cacaacatct cgatctcggc gcgtcccggc actctgacgg
     3181 cggtcatcgg cccttcaggt gcgggcaagt ccaccttcgc caagctggtc gccggataca
     3241 cccaaccgac aaccggcaca gtggcattcg agggccacaa cattcacgcc gaatatgcct
     3301 cgctgcgaag caggatcggc atggtgccac aggacgacgt ggtgcacggc cagctcacag
     3361 tcaatcaggc gctgatgtat gccgccgagc tgcggttgcc gcccgacacg accaaagccg
     3421 accgcgccca agtcgtcgcg caggttctcg aggagctcga gatgacccgg cacgccgata
     3481 ctcgcgtcga caagttgtcc ggcggtcagc gtaagcgtgc gtcggtggcg ctggagctgt
     3541 tgaccgggcc gtcgctgctc atcctcgatg agccgacgtc cggcctggac cccgccctgg
     3601 accggcaagt catgacgatg ctgcgcgatc tcgccgacgc gggccgcgtc gtgctcgtcg
     3661 tgacgcactc gctgacctac ctcgacgtct gcgaccaagt gctgcttctc gcaccgggcg
     3721 gcaagacagc gttcttcgga cccccgagtg agatcggcgc gtctatgggg acgaccaact
     3781 gggccgacat cttcagcacg gtcgcaagcg atccggacgc ggccaaccgc cggttcctcg
     3841 accagcacgg ccctccgcca ccgcccccgc ctgcggagca acccaaagac ctcggcagtc
     3901 cgacacggac cagcctttgg cggcagttct cgacgatcgc gcgacgccag gtgcgtctga
     3961 tcgtctcgga ccgcggctac ttcatcttcc tgatgatgct gccgttcatc atgggcgtgc
     4021 tggcaactac ggtgcccggc gaagccggat tcggcagccc cagtccgacg agcgagtcgc
     4081 cgaacgaacc gggaacgatc ctggtgctgc tcaacgtcgg cgccatcttc atgggcactg
     4141 cgctgacgat ccggaccctc atcggggagc gggcgatctt cctgcgcgag caggcggtcg
     4201 ggttgtccac gaccgcatac attttcgcaa agatgtgcgt cttcacggtg ttcgcggtgc
     4261 tacaggcgac catcgccgtc gcgatcacgg tgtggggcaa aggcgctccc actcagggcg
     4321 cggtgatgct gaagaacccg accctcgagt tgtggttcga tgtcgcattc acctgcgtcg
     4381 ccgcagcgat ggtcggcctg gcgctctcgg cactcgccag gtcgaacgag cagatcatgc
     4441 cgctgctggt ggtggcgatc atgtctcagc tggtgttctc gggcggcatg atcccggtga
     4501 ccgggcgcct tgtgctcgac cagctgtcat ggttgacgcc ggcccgttgg ggtttcgccg
     4561 cgtcggcgtc gacggcagat ctgctcaagc tggttcccgg tccggtgaca ccgaaggact
     4621 cgcactggaa acacacgccg tccgcgtgga tgttcgacgt tgggatgctt ttcgtgatct
     4681 gtgtcggcta cgcgacgttc gtccggtggc gaattcggct caagagttag ctcaggccgc
     4741 gagctcgtcc tccttttgta gttcgacgct gccgcacacc acttcttggc aacggcgggc
     4801 aagttcgcgg cggtccgtgc cgggcatctg caacgctccg acgtggatct cgaccacagt
     4861 ccgtcgcgtc gcgaccactc gccacaccga ccgagtcagc gtgtcctcgc cgacaaaagc
     4921 cggaaccgtc gacaagcggc cgtctcggtg gtggtagctc agtcgaagcg gcagcacggg
     4981 cctgtccgcg tcgatcgcgg cctgaaacat cgcaggccgg aacgggccat atcccagacc
     5041 gcaccacgac gtgccctccg ggaacgtcat gactgtccga ccggacttca gccgcgcggt
     5101 cacttgggcg acgactcccg gcaacggccg caacttcgtg cggtcgatcg ggatggcacc
     5161 cagagcccgc gccatctggc cgatgcccgg ccacttgatc atgtcggcct tcgcgacgaa
     5221 cgtggccggc atcaccgccc agatcaccag aacgtcggcc caggaaatgt gcgggctgac
     5281 caccagcgcc cctggcatat cgcgaattac gttgtccggc attgcgattc gcaccccaac
     5341 gcagcgaaga agaagctggc tgtaatgccg gacaagtcgg tcctgcccgg gatgcggaat
     5401 cgccgtgaac ggcatcagcg gcagaacggc gaaaatcaga aatgtgcgcc agcagccccg
     5461 gacgatccgc acaatgcgcc ccagaaaccg gacggggtcg tcgcggacgc agctctcgtc
     5521 gcagggagcg accggcaccc acgcatgcgt cattgctgaa tctcctcggc catttcgctg
     5581 gcggccgcca ccgacctgag gcggttgagg tagcggatgt cggcttcgcg cttgtccagc
     5641 agaaccatga aatcggccac gccgaagtcc gggtcatgcg cgggctcgcc gcacacgttc
     5701 gtgccgagcc gcaggtagcc acgcatcagc ggcggcacgg tcactcgcat gggcagcgga
     5761 atgccgtcga acgacaccgg tcggtacggc tgcacggtgt aggccgaacg gtgccgcttc
     5821 agcacgaagt cccgcacgcc acgtgcctgc tgtcccggca ggccgacgcc ctgaatcggc
     5881 acagacacgc aaccggtcac gtagttgtgt ccgcagtggt cgagataggc caaaattccg
     5941 gcccacatca agagcaccac cgcgccgttg cgatggtcgg cacgcaccac ggcccggccc
     6001 atctcgacca gcgacgcccg cagcgggtct agctcgtgta cgtcgaactc ggtggcggtg
     6061 tacagcccgc ccgctgcgac cgcccccgcc ggaggcagca cccgacagca gccgacgagt
     6121 tcgccggagc tgtcgtcgcg aatcagcaga tggtcgcagt actggtcgaa acagtcggca
     6181 tcactggcgg ccgtcagcgt gaaccctggt tccaaggtga acacctcata gcggagccgc
     6241 tgcgccgctt cgatgagcgc cggatcggtg gataagagca gcgagtactt gggtgcctcc
     6301 gaatcccggg agtcagaggc aagaaggaca gacgcggtgc tcatggctgc accgtcaagt
     6361 agcgcgatag ctgcgagatt tgctgagccg ggtcgtacat gtttgcagta ttccatcgcg
     6421 gatggcatgc cgccgggttt attgcccact tgttcagcga atcgtggtct gcaggttaca
     6481 tcaatgtctc tctgctaact gcccaggcca gccgtgccga tggccgccga gggcgtgcag
     6541 gccgctgtcg gccgactgtc aagcagacct cggcttccgg aaaaaacctt tctcccattc
     6601 cttttgggcc ttggctccct cacggatata gggcgtgaac ggcgcgatat gaaggatcag
     6661 ccaattgatg aacgatgccg gaaagcgcaa tggcttgacg aaatcgacga acaacacgac
     6721 gcgggtctgc tcggagtggt tccacacctc gtgctcgtac gagtcgtcga agatcagcac
     6781 cttgccttcg tcccaatggc agacctcgtt atcgacgcgg atggcgaccg tgccaggcgg
     6841 tgagttcggc acgatcagtc cgagatgtag gcggagcact ccgttgtaca cgccacgatg
     6901 cggtttgagt tgttttcccg gttcgaggat cgaaaacatt gccgtctgta gttgcgggat
     6961 cgtctgcacg atgcgccagg tctgcgggca ctgacgggca gcttcctccg acttggcgcc
     7021 gaatccgaca aataggaacg tcttccagtc ccggtcggtg ctgatcgctc ggacttcgcc
     7081 gatgatctca tggaagccgg gcaactcgtc cttgcgtgtc agcaggcgat cgagttcggc
     7141 gcggatcagg tgccactccc gctcgatttc ggttgcccaa gggaattcgg cggtggtgaa
     7201 gacgggtcgg ttgccgacct tcgagtattt ccaattgagc tgctcggccc acgaaacaag
     7261 gccgcccagc gctcggttca gaagcccggg acggctgatc gtatcttcga cgccgtcggt
     7321 gcgctgagtc tgagctttca ccgctgcagc ctttcctgac acattttggc gattggcaat
     7381 gaactgcaca tattaacgcg gcacgtggcg acgcatgcgg cgaatatgaa cccgacgcca
     7441 ctagctggcc aagctcgcca aggactgact tcgccgtggc ttaatccggc gaggattcgt
     7501 cgcggacggc ggcgcccgag accccaggag acaccgcatg aagaagtcag cagtggccat
     7561 gggcgttgcc atcgcgacca tctcgctcat cgtcggactc gttgggtgcg gcaaggattc
     7621 caaaacgagc accacctcga ccacgaccac gaccacgacc acgaccacga ccacgaccac
     7681 atccgcgcag gcgtcgggac ctcatcaaac actcggcgac tactcgaaag agaacaacat
     7741 ccaagagacc ctgataagcc gcggcactcc tggcgcgcca gtcgtcgacc tagggctgcc
     7801 tgacggatgg acggcgcagc ccgagagcaa cgacgcgccg tacggcgcca ttgtgttcga
     7861 taccccgtcc gatcccaacg acccacccag attccgcgcg ttcttcgaga agctgaccgg
     7921 caatgtcgac acagacaagc tactggccgc ttcgccgggc gaggtcctga acctgccggg
     7981 ttatgaaggt ggcgaaggcc aaagagacac actggctaac ttccccgcat attcggtcgg
     8041 cggcgaatac accaaggacg gggcgaagcg gatggccgcg cagaaggccg tggtgatcca
     8101 aggctcggac ggcatctatc tgcttct
//
LOCUS       DASWBF010000152         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_344588,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000152 DASWBF010000000
VERSION     DASWBF010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344588"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VF874_05145"
     CDS             complement(<1..246)
                     /locus_tag="VF874_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175495.1"
                     /translation="MRGRWQRRVLVVGPSELVASIIEDLCADTECGMKAVAACLSHQP
                     EQAGVSLPSGIPVLGTIDDTADIAHLVGADAVAVAASA"
     gene            complement(303..1424)
                     /locus_tag="VF874_05150"
     CDS             complement(303..1424)
                     /locus_tag="VF874_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9175496.1"
                     /translation="MAERVRVRDISNDEGNRLLRLVRRSSGSVVTWRRAQIVLLSAQG
                     MEVPQIAKVAFSSEDRVRDVLHNFNRDGFDSLYPRYAGGRPPTFTLPQRQEIKKVALG
                     RPTDHDLPFSTWSLTKLADFLVAKGVVDDISHEGLRLLLREEGVSFQVIKTWKCSTDP
                     DYEAKKNRVLQLYAIVDGRAQPGPADPTVVFCLDEFGPLNLQPHPGKQWAARGGGAEH
                     PRRRRRATYTRPHGVRHLLAGYDLTRDRLYGHVKPRKGRIEFLAFCRYLRSLYPPEVR
                     IAIVLDNFSPHLSTRRDQRVADWAAANNVELAYVPYYASWLNRIEAQFTALRYFALDG
                     TDHPSHQEQASMIRRYIAWRNRHKDNRRLREIVNKAKVA"
BASE COUNT          254 a          532 c          580 g          271 t
ORIGIN      
        1 tgcggatgcc gcgaccgcga ccgcgtccgc gccgacgaga tgcgcaatgt cggccgtgtc
       61 gtcgatggtg ccgagcaccg gtatgccgct gggcaggctc accccggcct gctcaggctg
      121 gtgcgaaagg cacgccgcga ccgccttcat cccacattcg gtgtccgcgc acaggtcctc
      181 gatgatgctg gcgactaact cactgggacc gacgacgagg acgcggcgct gccagcgtcc
      241 gcgcatcgcc accattcgcg accggcaacg ccaagccggt caccagccgg cctagtgccg
      301 cgtcaggcaa cctttgcctt gttgacgatc tcgcggagtc ggcggttgtc tttgtggcgg
      361 tttcgccagg cgatgtagcg gcggatcatg ctggcttgtt cttggtggct ggggtggtcg
      421 gtgccgtcga gggcgaagta acgcagggcg gtgaactggg cctcgatgcg gttcaaccac
      481 gaggcgtagt aggggacgta ggccagctcg acgttgttgg ccgccgccca gtcggcgacc
      541 cgctggtcgc ggcgggtgct caggtgcggg ctgaagttgt cgagcacgat cgcgatgcgc
      601 acctcgggcg ggtagaggct gcgcaggtag cggcagaagg caaggaactc gatccggccc
      661 ttgcggggct tgacgtgccc gtagagccgg tcgcgggtca ggtcgtagcc ggcgagcagg
      721 tgccgcaccc cgtgcgggcg ggtgtaggtg gcgcgccgcc ggcggcgcgg gtgctcggcg
      781 ccgccgccgc gggcggccca ctgcttgccc ggatggggtt gcaggttgag cgggccgaac
      841 tcgtcgaggc agaacaccac cgtcgggtcc gccggtccgg gctgggcccg cccgtcgacg
      901 atcgcataca gctgaaggac ccggttcttc ttcgcctcgt agtcgggatc ggtggagcac
      961 ttccaggttt tgatcacttg aaaggagacg ccctcctcgc ggagcagaag gcgcaggcct
     1021 tcgtggctga tgtcgtcgac cacccccttg gcgaccagga agtcggccag cttggtcaga
     1081 ctccaggtgg aaaacggcag gtcgtggtcg gtcggccgac ccaacgccac cttcttgatt
     1141 tcctgccgtt ggggcagggt gaacgtcggc ggtcgacccc ccgcatagcg cgggtagagg
     1201 ctgtcgaacc cgtcgcggtt gaagttgtgc agcacgtccc gaacccggtc ctcgctgctg
     1261 aacgcgacct tggcgatctg tggcacctcc atgccctgcg cggacagcag cacgatctgc
     1321 gcccggcgcc acgtcaccac cgaccccgag ctgcgccgca ccaagcgcag cagccggttg
     1381 ccctcgtcgt tgctgatgtc gcggactcgc acccgttcgg ccatcgccgc ctcctggggg
     1441 aagacagaag gaacgacggc agcattggat cagaccgcag ccaactgtgg gatcttgatc
     1501 ggcgagtcgc gtaacagggc gaaagttcaa gggcgcagca ctagacggcg agacgcccac
     1561 ctggatgacg tcgcggacgt cgatgaccta ccggacctgc gccggcctgc agcacggtca
     1621 tgagcgcctg cgccttc
//
LOCUS       DASWBF010000153         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_864423,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000153 DASWBF010000000
VERSION     DASWBF010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864423"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VF874_05155"
     CDS             <1..499
                     /locus_tag="VF874_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213895.1"
                     /note="catalyzes the reduction of mycothione or
                     glutathione to mycothione or glutathione disulfide;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HEX9175497.1"
                     /translation="VSSAYQLKHVANHEARVVRHNLLCDWDDTDSLIASDHRYVPSAV
                     FTDPQIASVGLTENQARAQGYTFRVKVQDYSDVAYGWAMEDTTGIAKIIVDDDTGLIL
                     GAHLMGHQASSLIQPIVQAMSFGLPAQDMARGQYWIHPALPEVIENALLALCGEPPWP
                     SSRRH"
     gene            707..1129
                     /locus_tag="VF874_05160"
     CDS             707..1129
                     /locus_tag="VF874_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX9175498.1"
                     /translation="MAKFDDSYPVRLRGDLDASVSRWLWLIKWLLLIPHYLVIAAIVS
                     GMVHLSDDFDDSGSATIPSFGVLVVIIIAALLFTGRYPRGLFDFLLGINRWMFHVRAY
                     ALLFRDEYPPFRLDQGPREPGAHSPPAREDAKNPKCSK"
     gene            complement(1160..2020)
                     /locus_tag="VF874_05165"
     CDS             complement(1160..2020)
                     /locus_tag="VF874_05165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175499.1"
                     /translation="MTTAERHARSTQEQDSDRQLKARHRALWALGDYPAVAAELIPEL
                     GPELVRVCGIRAGDRVLDVAAGSGNAAIPAAAAGAIVTASDLTPELFDAGRRIAAEHG
                     VDLEWVEADAEAMPFADNSFDFVMSCVGAMFAPHHQTAADELVRVCRPGGTIGMVNWT
                     PEGFIGNLFATMKPHAPPPPPGASPPPLWGDEAYVRKLFGERVTGLQARRREVVMDHC
                     ANPLEFREYWKRNYGPTIAVYRFNEDRPDRIEQLDRDFLSFLTAWNRAAEPGRAAYDA
                     EYLLVTAIKR"
     gene            complement(2711..>4865)
                     /locus_tag="VF874_05170"
     CDS             complement(2711..>4865)
                     /locus_tag="VF874_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEX9175500.1"
                     /translation="QVHQQILDGDHEVPARAVRTPDRPAVVDLPRRATSFVGRDQEME
                     RVITALREGPLVTLTGVGGVGKTRLALEAAWRGQKRFADGVWVCELAPLDASEAVSHA
                     IAATLRLQQQQGLGIEETVIEYLRTRELLLVVDNCEHVLDAAAALLAQIVQHCQRISV
                     LATSREALGIEGERVLLVPPLPVEDATRLFADRARASRPDFDLDNEPVGAVAEICQRL
                     DGLPLAIELAAARMRAMSSLDVARRLDGLRLLSGGTRGALPRQQSLAATIDWSYQLLT
                     EPEQSLFMRLSAFAGGWDLEAAHGVCGADGTSEDDTLELLTGLVDKSMVIVRSVLDRT
                     RYGVLETLRAYGRERLRENAIDDQYATRHTVYYTELVERAQAGMHGADERAWVERALP
                     DYDNMRAAFERAMADQDIDLALRLVTSLPELVHLRVGYESAGWAEKALEFADPNHPLF
                     PAAVGFAARGAWNRGDFDRARALAVLAGGRVPGRNTGRIAYPADVLADLSLYDGDPGA
                     ALTHYIGEVAHARRDADPIRLVWALFYVAICQAALRNPGAGLRAAQASVEVADETANP
                     TAQSMARYALGLVLKKSEPDRALALFDDAAQLAAAVRNFWWFGIAFMEAAATRGVHGD
                     PATAARMFIDVLDHWDRVGDWSQQWLNLRYVTRLLVRLGADDDAVALHHALVDAGKPS
                     PLSEEQVAALGDGNSDALSGAAAVIRARAALAGFV"
BASE COUNT          838 a         1664 c         1550 g          813 t
ORIGIN      
        1 cgtcagttcg gcctaccagc tcaagcacgt ggccaaccac gaagccagag tggtgcggca
       61 caacctgctg tgcgactggg acgacaccga ctcgctgatc gcgtcagacc accggtacgt
      121 gccgtcggcg gtgttcaccg atccgcagat cgccagcgtc ggtctgaccg aaaaccaagc
      181 gcgcgcacag ggttacacct tcagggtcaa ggtgcaggac tacagcgacg tggcatatgg
      241 atgggcgatg gaggacacca ccggtatcgc gaagatcatc gtcgacgacg ataccgggtt
      301 gatcctcgga gcgcatctga tgggccacca ggcgtcgtcg ctgatccagc cgatcgtgca
      361 ggcgatgagc ttcgggctgc ccgcccagga catggcccgc ggccagtact ggattcaccc
      421 ggcgctgccg gaggtgatcg agaacgcgct gctggcgctc tgcggcgagc cgccgtggcc
      481 gtcatcgcgg cgacattagc ggttcccacg agatgccgcc ttcgtcacgc cgttccacat
      541 cttggcctcc gactctggtt tcgtacgctc cgccggcgtg tcctgaacga aaccgcagag
      601 tcggcgcaga gaggcggcga ccgctgaccc gacagctgga tttccaattg gccgacgtcc
      661 ggcgtgtcgc ggatacccag acgcgggcaa ggtttacaac ggctgcatgg ctaagttcga
      721 cgacagctat ccggtgcggc tccgtgggga cctcgacgcc tctgtgtcgc gctggttgtg
      781 gttgatcaag tggctgttgc tcattccgca ctacctcgtg atcgcggcga tcgtcagcgg
      841 gatggtgcac ctgagcgatg acttcgacga ctcaggatcc gcgaccattc cgtcgttcgg
      901 cgtgcttgtg gtgatcatca tcgcggccct gctgttcacc ggccgctatc cccgggggct
      961 gttcgacttc ctgctcggaa tcaatcggtg gatgttccat gttcgcgcgt acgcgttgct
     1021 gttccgcgac gaatacccgc cattccggtt ggaccagggc ccgcgcgagc cgggagcgca
     1081 ctctcccccc gcgcgagaag acgcaaaaaa cccgaaatgt agcaagtagc ggggatttgc
     1141 gtcttgtcgc gccttgcagc taacgcttga tggcggtgac cagcaggtac tccgcatcgt
     1201 acgccgcccg accgggctcg gctgcccgat tccacgcggt gaggaagctc aagaaatccc
     1261 gatccaactg ctcgatccgg tccggccgat cttcgttgaa tcgatagacc gcgattgtcg
     1321 gtccatagtt gcgcttccaa tattcccgga actcaagcgg attcgcgcag tggtccatca
     1381 cgacctcccg gcggcgtgcc tgcagcccgg tgacccgctc tccgaacaac ttccgtacgt
     1441 acgcttcgtc gccccacaat ggtggcggac tggcgccggg cggcggtggc ggcgcgtggg
     1501 gcttcatggt cgcgaacagg ttgccgatga agccctccgg cgtccagttg accatcccga
     1561 tggttccccc tggccggcac acccggacca gctcgtcggc ggcggtctgg tgatgcggcg
     1621 cgaacatcgc accgacgcag gacatcacga agtcgaagct gttgtccgcg aacggcattg
     1681 cctccgcatc ggcttccacc cactcgagat cgacgccatg ctctgcggcg atccgacgcc
     1741 cggcgtcgaa aagctccggc gtcaggtcgc tcgcggtgac aatcgctccc gcggccgcgg
     1801 ccgggatggc ggcgttaccg gagcctgccg ccacgtccag gacgcgatct ccggcgcgaa
     1861 taccgcacac tcggacaagt tcgggcccca actcgggaat gagttcggcg gcgaccgccg
     1921 ggtagtcacc caacgcccac agggcgcggt gcctggcctt gagttgacgg tccgaatctt
     1981 gttcctgtgt cgacctggcg tgtcgttccg cggtggtcat ctcgtgtctc cttcagcgtt
     2041 gtgtctgcgg acgcgaataa cggtgccgag ccgtatttcg gcaacgcttc ggtgatgatg
     2101 aaatcggctg gtggcggtgc tgcggaggct tcgcgcggcg gagcgatccc agccgtaccg
     2161 gccaacgccg gccccgcgaa gcagcgcctg agctgcgaat ttcgcgaata gatcggcgtt
     2221 gccgaaaccc acgcttgcgt cgttggtgcg ttcaatcaac gggccacaca acgggcccac
     2281 gaaaccaagg agaagaactg cgcaccaacc agttccaggt gccgaagtga cgcaacgtca
     2341 ccgtcttcag agctgcggcg gtcgcactcg cgaccggcgc catcgcagcg cccgcggtct
     2401 cacacgcaga aaaagtgtgg gacatcggtc aatatgactc gtgcgtggca gcggcaaaca
     2461 acaggtatca gtctggcaaa accgatgtca cgacatgggg ggaagagatc aggttttgcc
     2521 gcgaccggtc cggcggggag tggactgaga gccaggggtg cacggcgcct ccagccacat
     2581 tccagaccca accgcaaacg ccacgctcgg tcgtggtgcc tgtgccgcgc gaagccgcgc
     2641 tgccctgacg gcgatggcga ccggccgggc ccagagatgc tgggagccgg ccggattcgt
     2701 gcacaacggc tcacacgaac ccggccagcg ccgcgcgcgc ccgaatgacg gccgcggcac
     2761 cgctcaaagc gtcggaattg ccgtcgccga gcgctgcaac ctgttcctca ctcaacggtg
     2821 acggcttgcc cgcatcgacg agtgcatggt gcaacgcaac ggcgtcgtcg tcggcgccca
     2881 ggcgcaccag caaccgcgtc acgtagcgca ggttgagcca ctgctggctc cagtccccga
     2941 cccggtccca gtggtcgagc acgtcgatga acatccgcgc cgccgttgcc ggatccccgt
     3001 gcaccccacg ggtcgccgcg gcctccatga acgcgattcc aaaccaccag aagtttcgca
     3061 ccgctgcggc tagctgcgcg gcgtcgtcga acagcgccag cgcccggtcg ggctctgact
     3121 tcttgagcac caggccaagc gcgtagcggg ccatcgactg cgccgtcggg ttggccgtct
     3181 cgtcggcgac ctccaccgac gcctgcgccg cacgcagtcc ggcgccggga ttgcgcagtg
     3241 cggcttggca aatcgcgacg tagaagagtg cccacaccag tcggatcggg tcggcgtcgc
     3301 ggcgggcgtg cgccacttcg ccgatgtaat gggtcagcgc ggcgcccgga tcaccgtcgt
     3361 agagcgacaa gtcggcgagc acgtccgcgg gatacgcaat tcgaccggtg tttcgaccgg
     3421 gaacacgtcc cccggcgagg accgccagtg cccgcgcgcg gtcgaagtcg ccacggttcc
     3481 acgcgccccg cgcggcgaaa ccgaccgcgg cgggaaacag cgggtggttg ggatcggcga
     3541 actcgagcgc cttttcggcc cagccggcag actcgtaacc gacccgcaag tgcacgagct
     3601 ctggcaatga cgtgaccaac cggagtgcaa ggtcgatgtc ctgatccgcc atcgcccgct
     3661 cgaacgccgc ccgcatgttg tcgtagtcgg gcagcgcccg ctcgacccac gcccgttcgt
     3721 ccgcaccgtg catgccagct tgggcgcgct cgacgagctc ggtgtagtac accgtatgcc
     3781 tcgtcgcgta ttgatcgtca atcgcgttct cccgcaagcg ctcacggccg taggcacgca
     3841 gtgtttccaa gacgccatat cgggtccggt cgagaacgct gcgcacaatc accatcgact
     3901 tgtcgacgag cccggtgagc aactcgagcg tgtcgtcctc ggacgtcccg tccgccccgc
     3961 acacgccgtg tgccgcttcg aggtcccacc cgcccgcgaa cgccgacagc cgcatgaaca
     4021 acgactgctc gggttcggtg agcagctggt aggaccagtc gatcgtcgct gccaggctct
     4081 gctgtctggg cagcgcgccg cgcgtgcctc cactcagaag gcgcaagcca tccaggcgtc
     4141 tggcgacgtc aaggctgctc atcgcccgca tccgggccgc cgccaattcg atcgcgagcg
     4201 gcaggccatc gagcctttga cagatctcgg caaccgcgcc gaccggctcg ttgtcgaggt
     4261 cgaagtcggg ccgacttgcc ctcgctcgat cggcgaacaa tctggtggcg tcctcgaccg
     4321 gcagcggcgg gactagcagg acccgctcac cctcgatgcc gagcgcctct ctactggtag
     4381 ccagcaccga tatccgctgg caatgttgga cgatctgtgc gagcaacgct gccgccgcgt
     4441 ccagaacgtg ctcgcaattg tcgacgacga gaagaagctc gcgcgtccgc aggtactcga
     4501 tcacggtctc ctcgatgccg agaccctgtt gctgctgcaa ccgcagagtc gccgcgatgg
     4561 cgtggctcac cgcctcactg gcatcgagcg gcgcgagttc gcatacccac acgccgtcag
     4621 cgaagcgctt ctggccgcgc catgcggcct cgagtgcgag ccgggtcttt ccgacaccgc
     4681 cgaccccggt caacgtcacc aacggaccct cacggagcgc ggtgatcacc cgctccatct
     4741 cctggtcccg gccgacgaag ctggtcgcgc gccgtggcag atcgacgacg gcaggacggt
     4801 cgggtgttcg cacggcccgc gcaggaacct cgtggtcgcc gtcgagaatc tgctgatgca
     4861 cctga
//
LOCUS       DASWBF010000154        12426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_344765,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000154 DASWBF010000000
VERSION     DASWBF010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344765"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1853)
                     /locus_tag="VF874_05175"
                     /pseudo
     CDS             complement(1..1853)
                     /locus_tag="VF874_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000690.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
     gene            2001..3173
                     /locus_tag="VF874_05180"
     CDS             2001..3173
                     /locus_tag="VF874_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX9175501.1"
                     /translation="MAADEGLALGVLGPLQMRVGGTEVPLGTPKQRAVLAMLVINRNR
                     PVSIESLLNAAWEQWPPSGARASLHSYVSNLRRLIGEGEVDARTILVSAAPGYRLNVR
                     ETDCDIGRFVTEKNAGVQAAAAGRFEQASRHLTAALSEWRGPVLEDLHNFQFVDAFAT
                     ALTEDKILAHTARAEAKIVCGRGYSVIGELEGLVVEHPYHEPLWAQLITAYYLSERQS
                     EALDAFNRLKAILADDLGIDPGPTVRDLQSRVLRQEPLDVKQAAQNTAERAITMLQQR
                     TLVHGEGALARLRDTSGRSFPLCAAATTIGRLADNDIVLDDASVSRNHAVIVDTGTNF
                     VISDLRSANGVRVGDHRVRGSAKLADGDHIGIGGHEFVFEIGAPQIAVSDADRGLA"
     gene            3250..4971
                     /locus_tag="VF874_05185"
     CDS             3250..4971
                     /locus_tag="VF874_05185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9175502.1"
                     /translation="MGTQFGPYHLKRLIGAGGFGEVYEAEDTVKERIVALKLLPEALS
                     KDPVFRKRMKREAHAAGRLQEPHVVPIHDYGEIDGLLFVDMRLIDGADLHALLEEKGP
                     LPPARAVAIIRQVAAALDAAHLAGVMHRDVKPENVLITRDDFAYLVDFGLATAASDER
                     LTQMGTMVGTFNYMSPERFRNKELTHRADIYSLACVLHECLTGRRPYRADSLSALITA
                     HLTAAIPRPSQIRPGIPVPFDQVIARGMAKNPEERYATAGDLANAALDALTATDKRQA
                     ARILERSEESTQPTGPPAAPPTQPYRPPPPTNRPAPTNRPAPTPPPPTNRPAPTPPAG
                     PTMGSGPIQRFAGPTSAGSQGNFGSGPLPVSAPPPWTPSRSQSGPHPVRRKRAPWLIG
                     GAVAAALVVAAVIVLFATGVFPVGGDGPPPPPPPDSSSVPPSGWTADDDTLIGYFTKP
                     GYNRNNCTPQHPPKDGTLSTLECDALSGGPDSATLSLYADQSALDDGFRNVTSSASLE
                     TCPDGTASPGKWHYTNDPTATAGQVACGMDGGDAVVVWSDDSKDFVAVVRGSNLKDLF
                     TWWQKES"
     gene            complement(4897..5814)
                     /locus_tag="VF874_05190"
     CDS             complement(4897..5814)
                     /locus_tag="VF874_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9175503.1"
                     /translation="MTRTPAHIGASMAVASMLCVQLGLAVSVALIDRVGVDGAAWLRL
                     AWAGLLLLIVVRPKRAAFTARAFAACVLLGAVTAGITLLFMAALDRIPMGTASALEFL
                     GPLGVAVAHGRARNLLWPALAAVGVVLLTQPWSGTVDPVGVAFALAAAVCWALYILLT
                     QRVGDDVAGINGLAVSMPVAALVATAVVGPSVIGRLTPELLLIGLGLAVLLPVVPFVL
                     EMLALRRLRAAAFGTLMSLEPAFALVIGLVILSQTPNAAAVAGIAFVVAAGIGAARSG
                     ARSAPLGLLLPPGEEVLEVRTANHRNEVL"
     gene            5898..6782
                     /locus_tag="VF874_05195"
     CDS             5898..6782
                     /locus_tag="VF874_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9175504.1"
                     /translation="MDTRRLWLLLELSRLGSMRAVADAHGLTTSTVSQQIAALGKETG
                     AQLVEPQGRRVRLTPAGRRLADHAVNILAAVDAARLDLDPDADPAGVLRVGGFATAIR
                     DSLLPILADLAHSHPRVEVVISEYEPDEAFALLADDKLDLALTYDYNLVPASPGPMLE
                     TVPLWSTPWGLGAQAEDAPTDIAAHAARPWIVNSRNTADEDAVRTLASLAGFSPRITH
                     QIDSLELVEDLILAGYGVGLLPLDRATREGVVVLPLTKPELVLTAYAATRRGRSNWAP
                     LRLVLDRLTMTVGCCDGR"
     gene            6841..7803
                     /locus_tag="VF874_05200"
     CDS             6841..7803
                     /locus_tag="VF874_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9175505.1"
                     /translation="MPASRPAIEAIDLVKKFGADVAVDGVSFTVPQGSVLGLLGPNGA
                     GKTTTVRMMTTLSQPTSGTARVAGYDVREQPDMVRRNMGLTGQVATVDELLTGRENIR
                     MIGGLYGIRRRDLAQLSDALLQQFSIADAADRPVKSYSGGMRRRLDLAVSLLAGPPVL
                     FLDEPTTGLDPRSRNDLWEVLRGLVREGTTLLLTTQYLEEADQLADNIVVMDKGRIIA
                     EGTPLQLKQQAGNASLVVTVSRPDDLAAAEALLRKTGAEVFIDTGARQLTAAADGLAD
                     MTRVAGWLRDSEIEVDDIGLSRPSLDDVFLSLTGHRTEAEEESA"
     gene            7800..8648
                     /locus_tag="VF874_05205"
     CDS             7800..8648
                     /locus_tag="VF874_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9175506.1"
                     /translation="MTAVTQQLPTKSHRPATHRTNIAQQSWIMVKRNMIHTKRMPEML
                     SDVTTQPIMFVLLFAFVFGASITNTGGPSYREFLLPGIQAQTIVFAAFVVAAGITNDI
                     EKGVIDRFRSLPIARSSVLIGRSVASLIHSSIGIVVMAVTGLAIGWRIRGGVAEAALA
                     FALVLVFGFGIIWFGILVGSLMRSVEAVNGVMFTVLFPITFLANTFAPTEPMSHWLRV
                     IAEWNPVSSLAQAMRELWGNGPPAPESAQLPLHHPVLATIIWSLLLTAVFAPVALRAY
                     ARRTAD"
     gene            complement(8655..9233)
                     /gene="pth"
                     /locus_tag="VF874_05210"
     CDS             complement(8655..9233)
                     /gene="pth"
                     /locus_tag="VF874_05210"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX9175507.1"
                     /translation="MAEPLLVVGLGNPGPVYAKTRHNLGFMVADLLAVRAGSTFKAHK
                     KSGADVATGRLAGRPVILGKPRSYMNESGRQVAALAKFYSVPAAELVVIHDELDIDFG
                     QIRLKFGGGVAGHNGLRSVASALSTNDFHRVRIGIGRPPGRKEPAAFVLEDFTAAERA
                     DVPVMCEQAADATELLIKLGLEAAQNQVHAWD"
     gene            complement(9237..9884)
                     /locus_tag="VF874_05215"
     CDS             complement(9237..9884)
                     /locus_tag="VF874_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX9175508.1"
                     /translation="MAKTNTNNLTAEVRTQTGKGASRRARRDGKVPAVLYGHGADPQH
                     LLLSAHDFAAVLRNSGTNAVLTLTIEDTEQFALTKALDIHPIRRNIQHADLLVVRRGE
                     KVTVDVNVIIEGEATPGALVTQDTTTIQIEAEALSIPEQLAVSVEGAEEGTQILASQV
                     QLPNGVTLVSDPDMLVVNVVTAPTAEELEPEAAEAAEEGEAESAAEAPGEAPDNE"
     gene            complement(10020..10889)
                     /locus_tag="VF874_05220"
     CDS             complement(10020..10889)
                     /locus_tag="VF874_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX9175509.1"
                     /translation="MTDWTAADLPAFAGRTVIVTGANSGLGLVTARELARVGAHVVLA
                     VRNTAKGDDAAAGIAGNVEVRKLDLQDLASVHEFADGVTAVDVLVNNAGIMAVPYAVT
                     KDGFESQIGTNHLGHFALTNLLLPKISDRVVTVSSVFHRIGRISLNDVNWKARPYRPW
                     LAYAQSKLANLLFTSELQRRLDEVGSDVRALAAHPGYSSTNLQGHAGGGGSSRLLSLG
                     DRLVATDADYGARQTLFAVAQDLPGNTYVGPRFGYLGPTQPVGRSPLARRSSTAKALW
                     ELSEQLTGTKFPL"
     gene            complement(10940..11596)
                     /locus_tag="VF874_05225"
     CDS             complement(10940..11596)
                     /locus_tag="VF874_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HEX9175510.1"
                     /translation="MTLHRSRRAATSCHRLAIALSLLVLLTGCDTKPTDYRSVWSSTP
                     TTTTTTEAAVPFSQYLQEKGVDGEPMTPQTLTELTVSMPHPPGWAVVTDASAPSAFEI
                     VRKTAVATYQPTAMLFVFKLTGGDFDPNDALKHAYDMPGAIQEPFNGMPSAKIEATYI
                     DATGQKLHRYNRIVIATARPPANQRYLIQFSVTTLGDETSEADPDVLAIIKGFTVAVR
                     "
     gene            complement(11593..11943)
                     /gene="arsC"
                     /locus_tag="VF874_05230"
     CDS             complement(11593..11943)
                     /gene="arsC"
                     /locus_tag="VF874_05230"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308942.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX9175511.1"
                     /translation="MAESDSVIYHNPRCSTSRNALKLLRDNGIEPRIVEYLNTPPTRG
                     QLADMIADAGIDVRTAVRKREEAYADLGLADASDDQLLDAMAANPILIERPFVVTPKG
                     TRLARPVDVLREIL"
     gene            complement(11943..>12426)
                     /gene="prsA"
                     /locus_tag="VF874_05235"
     CDS             complement(11943..>12426)
                     /gene="prsA"
                     /locus_tag="VF874_05235"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX9175512.1"
                     /translation="YSTDDMVVVSPDSGRVRVAEKWADALGGVPLAFIHKTRDPRVPN
                     QVVSNRVVGDVGGRTCVLIDDMIDTGGTIEGAVKLLHDDGARDVIIAATHGVLSHPAA
                     ERLQASGAKEVIVTNTLPIGEDKQFPQLTVLSIAPLLASTIRAVFENGSVTGLFDGEA
                     "
BASE COUNT         2041 a         4196 c         4184 g         2005 t
ORIGIN      
        1 gccagccttg caggccggtg agcatcgtca ggatcactag cttggaggcc aagtacgcgc
       61 cgcgcgacag gccgatggtg tgttcgggcg atagatcgcc tgctctttga cgatctcgcg
      121 aatcgaggcg gcgcagccca tcagtgcgcc gccgatgacc agtagcacca gcagctgccg
      181 tggctgctgg gattgctgcg cgatggcctg tgccaccgaa agcccggcgc tgcccggaac
      241 ggcatgcaag aacaggctca gcaccagcgg cagcgcgagc atgaacaccg agtactgccg
      301 gtcaccggcg atgaccgaga ggtaccgccg gcacagaatc gcgaactggt cgagcgggct
      361 ctgctgcgcg acggccgcaa tggcttagcg ggagcggagg cgacagcttg ctgcccgaac
      421 ggctgcgtgg cgggcctgat gtaggtcccg tacaagtggg agttcgccaa gcgggcggcc
      481 cagtcggtag tggtgtcgcg ttcgagcagc gtgaacaggt cgggaaagtc gacgcaaccg
      541 aaatactcga gcgcctgcgt cggcggcccg cagtacgcca tgcggccacc gggcgcgagg
      601 atgagcagac ggtcgcacat ctccagctgc gcaacgttat gggtaaccac caccaccgag
      661 cgaccgccgt ccgcaagccc gcgcagcatc tgcatcaccg acttgtcgta gccgggatcc
      721 agcccggagg ttggttcgtc aagaaacagc agcgatggct tggtcaacag ttccagcgcg
      781 acgctgacgc gcttgcgttg cccaccggac aagctggaga tgcgctgatt tgcctgggcc
      841 cgcagaccga gttcggtgag cacctcgtcg atccggttca atcgttcagc ggggctcacg
      901 tcgtctggaa agcgaagctg cgcagcgtaa ttcaatgccc gccacaccgt gagctgagta
      961 tgcagaatgt cgtcttgcgg cacgaaaccg attcggtgcc gcagttcggc gtagttctcg
     1021 tacaggtcac gtccggcgta gcagacctgc ccgacccgcg cagggcggaa ccccgtgagc
     1081 gcaccgagca gcgtggactt cccggcaccg gtcggcccga ccacggccaa gaagctgcgc
     1141 tgcggcaacg cgaagctgac attgtccagc agcacccggc ccttctcggt ggccaccgtc
     1201 agccctgacg cctcgaagga cacatcgccg gcgtcgatgt actcctcgag acggtcaccc
     1261 gccatgtgca ggcgctggtg gccgatcacc accaggtcgg tggggccaag cgccgcccgc
     1321 gcgatgcgga cgccgttgac gtaggtgccg ttagcgctgc cgaggtccac gagttcccac
     1381 tgctcgcccg accgtcgcaa ctcggcgtga tgacgcgaga cgagcagatc gcccaggacc
     1441 acgtcgttgt cgagtgcccg accgatcctc accgcgggcc gatcgatgtt gtgcaccgcc
     1501 gacggttcgc gcgcatcgcc gggctgcggc ggcgcgcttg gcgtcagttg gaccatcaca
     1561 ccgttgggca ccgggccgag ccacactgtc accgggccct tcaccacgag ctgttccagc
     1621 ggccggccgt cgacgaacat tccggccggg ctgtgattga tcagaaccca gccggccggc
     1681 gtcgcggtca gcgtcgcgtg atggcgcgat atcgatgggt tttgggtacg gacgtcgacg
     1741 tcgagggctc gcccgattcg ccatggacgg tcaggtgtcg cgtgccacac acggccctcg
     1801 acccgcagct cgagctgccg gcctgccgcg gcgggttcga ccgttccacc catgattacc
     1861 tccgggctga cgctaaccgt tcgcacgcgt gggcgcgtga aaaccgtatg gtcgcccgat
     1921 cgtcgattca tccaagcgcc gtcggcagcg cgggaattgg ctcacaatga gcgtggcgcc
     1981 gatgaaaggg ggccgctcgg atggcggcgg acgagggtct cgcgcttggt gttctcggcc
     2041 cattgcagat gcgcgtcggc ggcacggagg tgccgttggg cacgccaaag caacgtgcgg
     2101 tgctggcgat gttggtgatc aaccgcaacc gcccggtcag catcgaatcg ctgttgaacg
     2161 cggcgtggga gcaatggccg ccatcgggcg cgcgggccag cctgcattcg tacgtttcga
     2221 atctgcgcag gctgatcggc gagggagagg tcgacgctcg caccatcttg gtcagtgccg
     2281 caccgggata tcggctgaat gtccgcgaaa ccgactgcga catagggagg ttcgtcaccg
     2341 aaaagaacgc gggtgtgcag gcggcggccg cgggccgctt cgagcaggca agccgccacc
     2401 tgactgcagc gctgagcgaa tggcgcggcc cggtcctcga ggacttgcac aacttccagt
     2461 tcgtcgacgc gtttgcgact gccctgacag aggacaagat cttggcccac accgcccgcg
     2521 cggaagctaa aatcgtctgc gggcgtgggt attccgtgat cggcgagctc gaggggctcg
     2581 tcgtcgaaca cccttaccac gagccgctgt gggcgcaact gatcaccgct tactatctgt
     2641 ccgaacggca atccgaggcg ctggacgcgt tcaaccgtct caaagcgatc ctggccgacg
     2701 atctcggtat cgatcctggc ccgaccgtgc gcgacctcca atcgcgggta ctgcgccagg
     2761 agccgctgga cgtgaagcaa gccgcccaga acacggcgga gcgcgcgatc acgatgcttc
     2821 agcagcgcac gttggtccac ggcgagggcg cgctcgcgcg gttgcgcgac acgtccggac
     2881 gcagctttcc gttgtgcgcg gcggccacca cgatcggccg tcttgccgac aacgacatcg
     2941 ttctcgacga cgccagcgtc agccgcaatc acgccgtcat cgtcgacacc gggaccaact
     3001 tcgtgatcag cgatttgcgg tcggccaatg gagtcagggt cggagatcat cgagttcgcg
     3061 gtagtgccaa gctggccgac ggcgaccata tcggtatcgg cggccacgag ttcgtctttg
     3121 aaatcggcgc accgcaaatc gctgtttctg atgccgaccg ggggttagcg tgaaagccgg
     3181 caaaacgcgt gcccgggcta ccggcggtta gaggagacgg cgatgagcgg cgccccccag
     3241 ggttcgcgcg tgggtaccca gtttgggccc tatcacctga aacggttgat cggggccggc
     3301 ggcttcggcg aggtatacga ggcagaagac accgtcaagg aacgcatcgt cgcgctgaag
     3361 ctgctgcccg aggcgttgtc gaaggatccg gtctttcgca agcgcatgaa gcgagaggca
     3421 cacgccgccg ggcgtctgca ggaaccccac gtggtgccga tccacgatta cggcgagatc
     3481 gacgggctgc tgttcgtgga catgcgcctg atcgacggcg ccgaccttca cgcgctgctg
     3541 gaagagaaag ggccgctacc gcctgcccgg gcggtcgcga tcatccgtca ggtcgccgcc
     3601 gcgctggatg ccgcgcacct agcgggggtg atgcaccgcg acgtcaagcc ggagaatgtt
     3661 ctgatcaccc gcgacgactt cgcctatctc gtcgacttcg ggttggcgac cgcggccagc
     3721 gacgagcggc tgacgcaaat gggcacgatg gtcggcacct tcaactacat gtcccccgag
     3781 cgcttccgca acaaggaact cacccaccgc gccgacatct actcgttggc gtgcgtgctg
     3841 cacgaatgcc tgacgggaag gcgtccttac cgggcggaca gcctcagcgc gctgatcacc
     3901 gcgcacctga ccgcggcgat cccgcggccg agccagatac gcccgggcat cccggtgccc
     3961 ttcgaccagg tgatcgctcg cggcatggcc aagaaccccg aggagcggta cgcgaccgcg
     4021 ggcgatctgg cgaacgccgc gctcgacgcg ctcactgcga ccgacaagcg gcaggcggcc
     4081 aggattctgg agcgcagtga ggagtccacc caaccgaccg gaccgcccgc agcacccccg
     4141 acgcagccgt atcgaccgcc gccgcccacc aaccggcctg cgcccaccaa ccggcctgcg
     4201 ccgaccccgc cgccgcccac caaccggcct gcgccgaccc cgccggccgg gccgaccatg
     4261 ggctcgggcc cgatccagcg cttcgccggc ccgacctccg cggggagcca gggcaacttc
     4321 ggcagcggtc ccctgcccgt ttcggcgccg ccgccgtgga caccgtcgcg ttcccaatca
     4381 ggcccccacc cggtgcgacg caagcgcgcg ccgtggctca tcgggggcgc cgtggcggcg
     4441 gccctggtcg tggcggccgt gatcgtgctg ttcgcaacgg gcgtcttccc cgtgggcggt
     4501 gacggtccgc cgccgccgcc gccgccggac tcgtcgagcg tgccgccttc gggatggacc
     4561 gccgacgacg acacgctcat cggctacttc accaagccgg ggtacaaccg caacaactgc
     4621 accccgcagc acccgccgaa ggacggcacc ctttcgacgc tggaatgcga cgcgctcagc
     4681 ggaggtcccg acagcgcaac actctctctc tacgccgatc aaagcgcgct cgacgacggg
     4741 ttccggaacg tcacctccag cgccagcctc gagacctgcc ctgacggcac ggcgtcgcca
     4801 ggaaagtggc actacaccaa cgacccgacg gcgacggccg gccaggtcgc gtgcggaatg
     4861 gacggcggcg acgcggtggt cgtctggtcg gatgactcaa aggacttcgt tgcggtggtt
     4921 cgcggttcta acctcaagga cctcttcacc tggtggcaga aggagtccta agggcgccga
     4981 ccgtgctccg gaacgggccg cgccgatacc tgcggccacc acgaaggcga tgccggcgac
     5041 ggcagccgca ttgggcgtct ggctcaggat cacgagcccg atgaccaacg cgaatgccgg
     5101 ctccaggctc atcagcgtgc cgaacgctgc ggccctcagc cgccgcagcg ccagcatctc
     5161 caacacgaac gggacaaccg gcagcagcac cgccaagccg agtccgatca gcagtaactc
     5221 gggtgtgagc cggccgatta ccgacggtcc caccacggcc gttgcgacca gcgccgcgac
     5281 gggcatggag acggccagcc cgttgatgcc ggcgacgtcg tcgccgacgc gctgggtcag
     5341 caggatgtac agcgcccagc agacggcggc ggccagcgcg aacgccacgc ccaccgggtc
     5401 gacggtcccg ctccacggct gggtgagcag caccaccccg accgccgcaa gggccggcca
     5461 cagcaggttc cgggcgcggc cgtgggccac cgccacgccg agggggccga ggaattccag
     5521 cgcgctggcg gtgcccatcg gtatccggtc cagcgcggcc atgaacaaca gcgtgattcc
     5581 ggcggtgacg gcgccgagca gcacacacgc tgcgaaggca cgggccgtga acgcggcccg
     5641 tttggggcgc acgacgatca gcagcagcag gccggcccag gccagccgga gccaggcggc
     5701 gccgtccacc ccgacgcggt cgatcagtgc cacggacacg gcgagaccga gctgcacgca
     5761 cagcatcgac gcaaccgcca tcgatgcgcc gatgtgtgcg ggcgtgcggg tcattgctgc
     5821 attcaacggt atctcgactg ttcatgtcca cgtgaatttg gtggacatac cgttcatgaa
     5881 aagcgaacaa taccggtgtg gacacccgtc ggctctggct gctgctcgag ctgtcccggc
     5941 tcgggtcgat gcgcgcggtc gccgacgcgc acgggttgac cacgtcgacg gtgtctcaac
     6001 agatcgcggc gctgggcaag gagaccggcg cgcagctcgt cgaaccgcag ggccgtcgcg
     6061 tgcggctgac gcctgctggc cggcgcctgg ccgaccacgc cgtcaacatc ctcgcggccg
     6121 tcgacgccgc gcggctcgac ctcgatccgg atgccgatcc agccggggtg ttgcgggtcg
     6181 gcggattcgc cactgcgatc cgcgactcgc tgctgccgat cctggctgac ctggcccaca
     6241 gccacccacg cgtcgaggtg gtgatcagcg aatacgagcc cgacgaagcg ttcgcgttgc
     6301 ttgccgacga caagctggac ctcgcgctca cgtacgacta caacctggtg ccggcctcgc
     6361 ccggaccgat gctcgaaacc gtgccgctgt ggtcgacgcc ctggggtctg ggcgctcagg
     6421 cggaggatgc accgaccgac atcgccgcac acgctgcgcg gccgtggatc gtcaactcgc
     6481 gcaacaccgc tgacgaggac gcggtgcgca cgttggcgtc gctggctggt ttctccccgc
     6541 ggatcactca ccagatcgac agcctcgagc tcgtcgagga tctgattctg gccggatacg
     6601 gggttggctt gttgccgctt gaccgtgcca cccgcgaggg cgtcgtggtg ctgccgctga
     6661 ccaaaccgga actcgtgctg accgcgtacg cggcgacgcg gcgggggcgg tcgaactggg
     6721 cgccgctgcg gctggtgctc gaccggctca cgatgactgt tggctgctgc gatggtcggt
     6781 agtggcgtgc agccgaaaac gaatggcacg gtgtcggggc ggatagatac cgtcgaagct
     6841 atgcccgcct ccaggccggc catcgaagca atcgatctgg tcaagaaatt cggcgccgac
     6901 gtcgctgtcg acggtgtgag tttcaccgtg ccgcaaggca gtgtgctggg cctcctgggt
     6961 cctaatggcg ccggcaagac caccaccgtg cggatgatga ccacactcag ccaacccacc
     7021 agcggcaccg cgcgggtggc cggctacgac gtgcgcgagc agccggacat ggtgcgacgc
     7081 aacatggggt tgacgggtca ggtggcgacg gtcgacgagc tgctcaccgg ccgggagaac
     7141 atccggatga tcggtgggct ttacggtatc cgccgccgcg atctcgcaca gttgagcgac
     7201 gcactgctgc aacagttttc gatcgccgac gccgctgacc gcccggtcaa gtcgtattcg
     7261 ggcggcatgc gccggcgcct ggatttggcc gtgagcctgt tggccggtcc accggtgctg
     7321 tttctcgacg agcccaccac ggggctggat ccccgcagcc gcaacgacct atgggaagtg
     7381 ttgcgcgggt tggtgcgcga gggcaccacg ctgctgctga ccacgcagta tctcgaggag
     7441 gccgaccagt tggccgacaa catcgtcgtc atggacaagg gccgcatcat cgccgagggc
     7501 acgccactgc agctcaagca gcaggcgggc aacgcgagcc tggtggtcac ggtgtcgcgc
     7561 cccgacgacc tcgccgccgc ggaagcgctg ttacgcaaga ccggcgccga ggtgttcatc
     7621 gacaccggcg cgcgccagct gaccgccgcc gccgacggcc tggccgatat gacccgggtc
     7681 gcaggctggt tgcgcgacag cgagatcgag gtcgacgaca tcgggttgtc gcgccctagc
     7741 ctcgacgacg tgttcctgtc actgaccgga caccgcaccg aagcagagga ggagtcggca
     7801 tgaccgctgt tacccagcaa ctgcccacga agtcgcaccg gccggccact caccgcacca
     7861 acatcgcgca gcagtcatgg atcatggtga agcggaacat gattcacacc aagcggatgc
     7921 cagagatgct gagcgacgtg accacgcagc ccatcatgtt cgtgctgctg ttcgcgttcg
     7981 tgttcggcgc gtcgatcacc aacaccggcg ggccgtccta ccgcgagttc ctgctgccgg
     8041 gcatccaggc gcagaccatc gtgttcgcag cgttcgtcgt cgccgcgggg atcaccaatg
     8101 acatcgagaa gggcgtcatc gacaggttca ggtcgctgcc tatcgccagg tcgtcggtgt
     8161 tgatcggacg cagcgtggca agcctgatcc attcgtcgat cggcattgtc gtgatggcgg
     8221 tgactggcct ggccatcgga tggcgcatcc gcggcggcgt cgcggaggcc gcgctggcgt
     8281 tcgcactcgt gctggtgttc gggttcggca tcatctggtt cggcatcctg gtcgggtcgc
     8341 tcatgcggtc ggtggaggcg gtcaacggcg tgatgttcac cgtgctgttc ccgatcacgt
     8401 tcttggccaa cacgtttgcg ccgaccgagc cgatgtcgca ttggctgcgg gtgatcgccg
     8461 agtggaaccc ggtgtcgtcg ctggcgcagg cgatgcgcga actgtggggc aacgggccac
     8521 ccgcgccgga atcagcgcag ctgccgctgc accacccggt gctcgcgacg atcatctggt
     8581 cgctgctgct gaccgcggtg ttcgcgccgg tggccctgcg cgcctacgcg cgccgcaccg
     8641 ccgactgacg ccgttcagtc ccaggcatgc acctggttct gggcggcctc gaggcccagc
     8701 ttgatcagca actcggtggc gtcggcggct tgctcgcaca tcaccggcac gtccgcgcgc
     8761 tcggcggcgg tgaagtcttc cagcacgaac gcggccggct ccttgcggcc cggcggccgt
     8821 ccgatgccga tgcgcacgcg gtgaaagtcg ttggtgctca acgccgacgc gaccgaacgc
     8881 aacccgttgt ggcccgcgac accgccgccg aacttcagcc ggatctggcc gaagtcgatg
     8941 tcgagctcgt cgtgaatcac gacgagctcg gccgccggca ccgagtagaa cttggccagc
     9001 gcggcgacct gccgtccgga ttcgttcatg tagctgcgcg gcttgccgag tatcaccggt
     9061 cgcccggcca gtcgcccggt cgcgacgtcg gcaccggatt tcttgtgcgc cttgaacgtt
     9121 gagcccgcgc ggaccgccag cagatcggcg accatgaacc cgaggttgtg ccgggtcttg
     9181 gcgtagaccg gccccggatt gcccaggccg accacgagca acggctcggc caccggctac
     9241 tcgttgtcgg gcgcttcgcc cggcgcttcc gccgccgact cggcttcgcc ctcctcggcg
     9301 gcttccgcgg cttcgggctc gagttcctcg gcggttggcg ccgtgacgac gttgaccacc
     9361 agcatgtccg ggtcggacac cagcgtcacg ccgttgggta gctgtacctg gcttgccaag
     9421 atctgggtgc cctcctcggc gccttcgacg gagacggcta gctgttcggg gatcgacagc
     9481 gcctcggcct cgatctggat cgtggtggtg tcctgcgtga ccagcgcgcc gggcgtcgcc
     9541 tcaccctcga tgatcacgtt gacatcgacc gtgaccttct cgccgcggcg caccacgagc
     9601 aggtcggcgt gctggatgtt gcggcggatc gggtgtatgt cgagcgcctt ggtgagtgcg
     9661 aattgctcgg tgtcctcgat ggtcagggtg agcacggcgt tggtgcccga gttgcgcagc
     9721 acggcggcga agtcgtgcgc ggacagcagc aggtgctggg ggtctgcgcc gtggccgtac
     9781 agcaccgcgg gcaccttgcc gtcgcgacgc gcgcgccgcg acgcgccctt gccggtctgc
     9841 gtgcggacct cggcggtcaa gttgttggta ttggtcttcg ccatctggat gctcctatcg
     9901 caagtcgtcg cacgacgcgg gagaaggcaa ccagacgcgg atgatcgcgg gtctccgtcg
     9961 ataacggtgg ctgcaaccac cctcgccgtg acgtccagca agggtagccc aggagcgcgt
    10021 cagagcggaa atttggtgcc cgtgagctgc tcggacagct cccacagcgc cttggcagtg
    10081 ctcgatcgcc gggccagcgg gctgcggccc accggctgag tggggccgag gtaaccgaac
    10141 cgcgggccga cgtatgtgtt gcccggcagg tcctgcgcta ccgcgaacag cgtctgccgt
    10201 gcgccgtagt cggcgtcggt ggccaccagc cgatctccca ggctcagcag cctggaactc
    10261 ccgcccccgc cggcgtggcc ctgcagattc gtcgacgagt agccgggatg ggcggccagt
    10321 gcccgcacgt ccgagcccac ctcgtcgagg cggcgctgca gctcgctggt gaacaacagg
    10381 ttggccagct tggactgcgc gtacgccagc cacggccggt acggccgcgc cttccagttc
    10441 acgtcgttga ggctgatcct gccgatgcga tggaacaccg acgacaccgt gaccactcgg
    10501 tcgctgatct tcggcaacag caggttcgtc aacgcgaagt ggccgagatg gtttgtgccg
    10561 atctggcttt cgaacccgtc cttggtcacc gcgtacggga cggccatgat gccggcgttg
    10621 ttcaccagca cgtcgaccgc ggtcacgccg tcggcgaact cgtgcacaga cgccaggtcc
    10681 tgcaagtcca acttgcgcac ttcgacgttg ccggcgatgc ccgccgcggc atcgtcgccc
    10741 ttggcggtgt tgcggaccgc aagcacgaca tgcgctccga cacgcgccaa ttcgcgcgcg
    10801 gtgaccagcc ccagtccact attcgcgccg gtgacgatca cggtgcgtcc ggcgaacgcg
    10861 ggcaggtcgg cggctgtcca gtcagtcatg gggtcaccct aacgggccac gtccaagcgg
    10921 gactggcgag gtgctcccgt cagcgcacgg cgactgtgaa ccccttgatg atggccagca
    10981 cgtcgggatc ggcttcggag gtctcgtcgc ccagcgtggt gaccgaaaac tgaatcaggt
    11041 agcgctgatt cgccggaggt cgcgcggtcg cgatgacgat gcggttgtag cggtgaagtt
    11101 tctggccggt ggcgtcgata taggtggcct cgatcttcgc cgacggcatg ccgttgaacg
    11161 gttcctggat cgcgccgggc atgtcgtatg cgtgcttcag cgcgtcgttg gggtcaaagt
    11221 caccgccggt cagcttgaac acgaaaagca ttgcggtggg ctgatacgtc gcaaccgcgg
    11281 tcttgcggac gatctcgaac gcggacggcg cgctcgcatc cgtgaccaca gcccatcccg
    11341 gcggatgcgg catcgacacc gtcagctcgg tgagcgtctg cggtgtcatc ggttcgccgt
    11401 caaccccctt ctcctgtagg tactgggaga atgggacggc ggcttccgtg gtggtagtcg
    11461 ttgtcggcgt cgacgaccac accgagcggt agtcggtggg tttggtgtcg catccggtca
    11521 gaagaaccag cagcgacagc gcaatcgcga gtcggtggca ggatgtcgcc gcccggcggc
    11581 tccggtgcag cgtcacaaga tctcgcgcag cacgtccacc ggccgcgcca gccgggtgcc
    11641 tttgggcgtg acgacgaacg gtcgctcgat caggatcggg ttggcggcca tcgcgtcgag
    11701 cagctggtcg tcgctggcgt cggcaagtcc gaggtcggcg tatgcttcct cacgtttgcg
    11761 caccgccgtc cgcacgtcga tcccggcgtc ggcgatcatg tcggcaagct gcccccgcgt
    11821 cggcggcgta ttcaggtatt ccacgatccg tggttcgatc ccgttgtcgc gcaacaactt
    11881 cagcgcattg cgcgacgtcg agcagcgcgg gttgtggtag atgaccgagt cgctttcggc
    11941 catcacgctt ccccgtcaaa aagccctgtc acagaaccgt tttcgaacac cgcgcggatg
    12001 gtgctggcca gcagcggcgc gatcgacagc acggtcagct gcgggaactg cttgtcttcg
    12061 ccgatcggca gcgtattggt gacgatcacc tctttggcgc cgctggcctg cagccgctcg
    12121 gccgccggat gcgacagcac gccgtgcgtc gccgcgatga tcacgtcacg cgcgccgtcg
    12181 tcgtgcagca gcttcaccgc tccctcgatg gtgccgccgg tgtcgatcat gtcatcgatc
    12241 agcacgcagg tccggccgcc cacgtcgccg accacccggt tggacaccac ctggttgggg
    12301 acgcgcgggt cgcgcgtctt gtggatgaac gccagcggaa ccccacccaa tgcgtcggcc
    12361 cacttctcgg cgacgcgcac ccggcccgaa tcgggggaga ccacaaccat gtcgtcggtc
    12421 gaatag
//
LOCUS       DASWBF010000155         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_937579,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000155 DASWBF010000000
VERSION     DASWBF010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937579"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..878)
                     /locus_tag="VF874_05240"
     CDS             complement(324..878)
                     /locus_tag="VF874_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX9175513.1"
                     /translation="MEEALTGRAGPHRCTRVRSWRAWRNTVARAAPQLLVVLADTQMR
                     SGTLNLVIGSKSRLALPSVSSRGLGTANAPAPVVLLCACSSNVTGDVLGAVPSALVDR
                     GAAAVVATLTSVGGQHAAAAAAAVVHAMHSDRLSNPTLAAALTQARQRLIAFGSPIGL
                     LLAAHGDIDITLTTQNRQTSRQAR"
     gene            1204..1581
                     /locus_tag="VF874_05245"
     CDS             1204..1581
                     /locus_tag="VF874_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175514.1"
                     /translation="MAIVRASVKPTSASLSASLLPRRARLISSLTAAGSSSLVGASLA
                     DSTFDADSAMTEVISSMADNGISIRSSVPSRFRVVAVHSTVTSAVAVSLRGSGAALSG
                     TCNNRMRSRHEELARTQASRRAA"
     gene            complement(1613..>2660)
                     /locus_tag="VF874_05250"
     CDS             complement(1613..>2660)
                     /locus_tag="VF874_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175515.1"
                     /translation="VTGLAQSEAQLVSPALLVVNFGAVRCDAVVCTRQYDGGYAAVAA
                     RSVHRLGGFMLEARVRQWVRRELQSSSAALLSALDELRPGTNVRLDTAIREAIGALST
                     DASADIRIDSKPDPIVIRLDRDEFDVLTRGYVDAAVELTRDMLAVADHMKTSSAHTII
                     ILTGAAARNPTIRTRIAQLGFVADFGDPATIIARGATYHGAQTAASAALSRSSAVSVG
                     GPRAAVFPPPQSPADPEPSEQPPAERARVRTLLVNVTRDGRVCRTSLAPHCEHHLEVR
                     IAVARRGEEPPGVDHAAMPDDERAVELLVDVSSDDGTVHGTASVVLPMCDRNRASTVA
                     VVNSPRATMDRLLG"
BASE COUNT          481 a          850 c          879 g          450 t
ORIGIN      
        1 ccgccatcga cttctaccgc acgcaggtgt ttccgaaact gcactgactg tcgccttgcc
       61 gccagggagc ggctgtacga cgtcaacgac ctccggtcac cctgatgcgc agggaagcca
      121 gcgcccacgt ggggggttga atgcagtcag acgcgcccaa catgggccgc gaagagcgcc
      181 gcgcgctgct tgagcggcgg cgcgcagcgg ttgcacgcca attgcggcgt ctggcaatcg
      241 agttaatgga tctcgacaga cagcctgacg agatggagca ggcggaccgt tgactcgagc
      301 gccttgagcg gcagggcgct cactcagcgg gcctgccgcg atgtttggcg attttgcgtg
      361 gtgagcgtta tgtcgatgtc gccgtgggca gcaagcagca atccgatggg cgatccgaag
      421 gcgatcaacc gctgtcgcgc ctgggtcagc gccgccgcca gcgtcgggtt ggataggcgg
      481 tcggaatgca tggcatggac aacggccgcc gcggccgccg ccgcatgctg acctccgacg
      541 ctggtcaacg tggcgacgac tgcagcggcg ccccggtcaa cgagggcgct gggaaccgcg
      601 cccaaaacgt caccggtcac attggagctg catgcgcaca gcaagaccac cggcgctggc
      661 gcgttggcag tgccgagtcc gcgagaagag accgaaggca gggccaaccg cgacttgctg
      721 ccgatgacga ggttcaatgt cccgctgcgc atttgcgtgt cggcgagcac gaccagtagc
      781 tggggcgcgg ctcgtgccac ggtgttgcgc catgcgcgcc agctgcgcac ccgggtgcag
      841 cggtgaggtc ccgcgcgacc ggtgagcgcc tcttccacct tcttggtcgg tgtctcccat
      901 ggcgggccga attcgtcagc gcaatcagcg gcgccgtaaa ggactgggcg aagtgaaagg
      961 ggcgccagct gtgggcgggg cggtagccgt tttatcggcc tgccgccgac agtacgagcg
     1021 atcacccggt tcattgccca gaatgcgtag gggcacaccg tctgctgtga cgcatgtgaa
     1081 gtcggtgcag ccgggcgcgc ccctcgatct gagacacacg cacataaccg ggcgctttgg
     1141 tcgggtgcta ccccgtcata ggccaattcc agtggcacga cgggagagtc ataccgagcc
     1201 ttcatggcga ttgtccgcgc atcggtcaag ccaacgtcgg ccagtttgtc ggccagcctg
     1261 ctacctagac gcgcgagatt gatcagcagt ctcactgccg cgggttcgtc cagcctggtg
     1321 ggagcgtcgc tggcggacag cacctttgat gcggactctg ccatgaccga ggtgatctcc
     1381 tcaatggcgg acaacgggat ttcgatccgc tcctcggtgc cgagtcgttt cagggtggtc
     1441 gctgtgcact ccacagtcac gtcggctgtt gctgtatcgc tgcgcggctc cggtgcggcg
     1501 ctcagcggca cctgcaacaa ccggatgcga tctcgccacg aagaattggc tcgaacccaa
     1561 gccagcaggc gagccgcctg aatcggtcgg ccgtcatgga gaacggtgat gttcagccca
     1621 agagtcgatc catcgtcgcc cgtggcgaat taacaaccgc cactgtgctg gcacgattgc
     1681 ggtcgcacat aggcaaaacg accgacgcgg taccgtgcac ggtcccgtca tcgctggaga
     1741 cgtctaccag caactcgacg gcccgctcgt catcaggcat cgcagcgtga tcgactccgg
     1801 ggggttcctc gccgcggcgt gctaccgcaa tgcgaacctc taggtggtgc tcgcaatgcg
     1861 gcgccaatga cgttcgacac acccggccgt cacgcgtcac attcacaagt agcgtacgga
     1921 cgcgcgccct ttcggcaggt ggttgctccg agggttcagg atcagccggg ctctgcggag
     1981 gtggaaacac agcggcccgc gggccgccga cactcaccgc agaagatcta ctcaacgccg
     2041 cggaagcagc ggtctgtgcg ccgtggtagg tcgcgccccg ggcgataatc gttgccggat
     2101 caccgaaatc agcgacgaaa ccaagctgcg cgatgcgtgt acggattgtc gggttccggg
     2161 cggcggcacc ggtcaagata ataatggtgt gcgccgagga ggttttcatg tgatcggcga
     2221 ccgccagcat gtcacgcgtc aactcgaccg ccgcgtcgac atagccgcga gtgaggacgt
     2281 cgaattcgtc gcggtccagc cggatcacga ttgggtcggg cttgctgtcg atgcggatgt
     2341 ctgccgaggc gtccgttgac agtgcgccga tcgcctcgcg gatggcggta tccagccgca
     2401 cgttggtgcc gggacgcagt tcgtcgagcg ccgatagcaa cgccgcagaa gaactttgca
     2461 gttcgcggcg cacccactgg cggacacggg cctctaacat aaacccgccc aatcgatgga
     2521 cgctgcgggc cgctactgcg gcgtagccac cgtcatactg gcgggtgcac accaccgcat
     2581 cgcatctcac cgcgccgaaa ttgaccacca gcagcgccgg cgaaaccagc tgcgcctcac
     2641 tttgcgccaa cccggtgacc
//
LOCUS       DASWBF010000156         6820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_672449,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000156 DASWBF010000000
VERSION     DASWBF010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672449"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..196
                     /locus_tag="VF874_05255"
                     /pseudo
     CDS             1..196
                     /locus_tag="VF874_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611430.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(418..1530)
                     /locus_tag="VF874_05260"
     CDS             complement(418..1530)
                     /locus_tag="VF874_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175516.1"
                     /translation="MGRRQVALEGVELVQRQIENAMQLRDAIDNNPLLSRYMACLRTS
                     DLIPAEFRPSAIAQPLRSGLRNMMTAWDADEFVLDPSRITLSIGRTGYDRDTFKRDQL
                     MDRHGVQINKTSRNTVLFMTNIGTTRSSVAFLVEVLVKIAGELDQRISEMGLGERGRF
                     EQKVRRLTGHSAAMPDFSGFHPAFRDHSSIEDTPEGDVRRAFYLSYDDNTCEYLTGEQ
                     IDEKLNAGVDVVSATFVTPYPPGFRVLVPGQLFGRQILSFIRDLDTPEIHGYKPEFRV
                     PGVHRESDRNGQSGDCVAHQRTPATGPRTGRASVRPGLSTVSEMPAGNGILERRAHDT
                     GDRCHLRRKRLNASERQGFETRSDPDSYPVGGRPCG"
     gene            1728..1895
                     /locus_tag="VF874_05265"
                     /pseudo
     CDS             1728..1895
                     /locus_tag="VF874_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1953..2042
                     /locus_tag="VF874_05270"
                     /pseudo
     CDS             1953..2042
                     /locus_tag="VF874_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2643..2831
                     /locus_tag="VF874_05275"
     CDS             2643..2831
                     /locus_tag="VF874_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175517.1"
                     /translation="MPTRRRTRAADRARRIREQRTLNAAHRAQYAATNTTECDKPPPT
                     EPWNIDTNTDAGDEPPPF"
     gene            2918..3172
                     /locus_tag="VF874_05280"
     CDS             2918..3172
                     /locus_tag="VF874_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175518.1"
                     /translation="MTPAGRMELTPQQLDAIAWDFLRSEFTAPIYADWPIDRRFDAYL
                     HHQGRDDLVNDGAAYDALLQEVMVNIGPAVRRGLLGSHMN"
     gene            3179..4978
                     /gene="acsA"
                     /locus_tag="VF874_05285"
     CDS             3179..4978
                     /gene="acsA"
                     /locus_tag="VF874_05285"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9175519.1"
                     /translation="MDIIRKSPEDLKVTPNFLDYERTRADFRWSDAPPLCEGMGPGGC
                     NIAFAAVDRHADGPAATRTALRFIPEGSWDGTLASRDLSYAELARLARRFTNVLRSLG
                     INKGDRVFTIMGRRPELYITMLGALRNGSIVSPLFSAFGPEPIATRVEIGEATVLVTT
                     AGIYHRKIAKIRDQLTSVRHIFIVDGDKTNDDDRGTLNYWHWMNAADDNAPIEPTTAD
                     DPALLHFTSGTTGTPKGAIHVHGAVTMHYLTGLYALDLHDDDTFWCTADPGWVTGTSY
                     GVITPLLHGVTSIVDEAEFDAERWYRILQDQGVTVWYTAPTAIRMLIKAGPEIAARYS
                     FPRLRFVASVGEPLNADAVWWGKRVLGLPIHDNWWQTETGGIMVANTPAFDIKPGSMG
                     RPLPGVDAFLVHRNDDGTVTVLDEPDVEGELALRPGWPSMFRGYLHEDERYRKCFVDN
                     LYLTGDLAKRDSDGYYWFVGRADDVIKSSGHLIGPFEVENVLTEHPAVAEAGVIGKPD
                     PTVGEMVKAFVSLKDGFTADETLRRELLGHARKKLGASVAPKEIEFVDALPHTRSGKI
                     MRRLLKARELGLPEGDTSTVETAAPAPDEKVTL"
     gene            4975..5952
                     /gene="pdhA"
                     /locus_tag="VF874_05290"
     CDS             4975..5952
                     /gene="pdhA"
                     /locus_tag="VF874_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX9175520.1"
                     /translation="MTDADLARSLLSDMVRIRRMEEKCAELYSEAKIRGFLHLYIGEE
                     AVAAGSLQALATEDAVVATYREHAHALLSGIPMTSIMAEMFGKQEGCSRGRGGSMHLF
                     DADLRFYGGNAIVAGGLPLAVGLGLADAMLHRNRVTACYFGDGAVAEGAFHESLNIAA
                     LWQLPVLFCCENNLYAMGTALERAQSQTDLAAKASAYKVSTTVVDGMDVIACYEAARR
                     GAEAVRATGGPFFIEFRTYRFRAHSMFDPELYRDKAEVELWRQRDPIQTFTERCLRDG
                     LLTEDDVKQIEQAALDEVADAVAYAEAGTWESVEDLERDVMTPVSEAAR"
     gene            5949..>6820
                     /locus_tag="VF874_05295"
     CDS             5949..>6820
                     /locus_tag="VF874_05295"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9175521.1"
                     /translation="MKTTYRTAVHDGIRDAMRDDPRVVLMGEDVGRYGGTYAASKGLL
                     EEFGPERVRDTPLSELGFVGVGIGAALGGLRPIVEVMTVNFSLLALDQIVNTAAALRH
                     MSGGQFSVPLVVRMATGAGRQLAAQHSHSLENWYAHVPGLKVVAPATVADAYGMLGPA
                     LADPDPVVIFEHVQLYNAAADVDTLAPTDISSAAIRRAGSDVTVIAYGGCVPKALDAA
                     NELSLAGIDSEVIDLRVLRPLDTATIVESVRKTHRAVVVDEAWRSGSLAAEISARIVE
                     DAFFDLDAPIARVCT"
BASE COUNT         1245 a         2235 c         2193 g         1147 t
ORIGIN      
        1 cgcgcactgc ctgggcgcgc tgtacaaagc ggccagccaa gggctaccga cgttggcgga
       61 caagggatac gaaggttccg ggatcggcgt gcacactccg gtcaagggcc gcgacctcga
      121 cgtcgagaac cgaagctaca acacgctgtt gaccgcggcg cgggccatcg gcgaacgtgc
      181 caacgccgaa ctcaagcaac gctgaagccc tagccctagc agccgctcca ggtaacgcac
      241 agctgaacgc aagttgaccg ttagcactct cgggcgcaag ctctcccaac ggccgagatc
      301 agcgagcagc tgttaaattt aattgccgac gtcgagcgcc ccctgaccaa aaagtatgcg
      361 aatagcccgc ccgatcagtg caggcagcgc cggttgagga tgcgatggcc gcgtggacta
      421 cccgcacggt cgtccgccaa cggggtaaga atctggatcg ctccgagtct cgaatccttg
      481 ccgttcggag gcgttcaggc gcttacgtcg gagatggcat cgatcgcccg tatcgtgcgc
      541 gcggcgctcc aggatgccat ttcccgcggg catttccgac acggtgctta ggccggggcg
      601 gacactggct ctgccggttc gggggccggt ggccggtgtc cgttggtggg cgacacagtc
      661 gcccgactgg ccatttcgat cgctttctcg gtgtacaccc ggaacccgaa attcgggctt
      721 gtaaccatgg atctcggggg tgtccagatc gcggatgaag gacaggatct ggcggccgaa
      781 aagctgcccg ggaacgagca cccggaatcc cggcgggtag ggcgtgacga acgtcgcgga
      841 caccacgtcg acaccggcgt tgagcttttc gtcgatctgt tcgccggtca ggtattcgca
      901 ggtgttgtcg tcgtaggaca ggtagaaggc ccgccggacg tcgccttcgg gggtgtcctc
      961 gatgctgctg tggtcgcgga aggcgggatg gaacccgctg aagtcgggca tcgccgccga
     1021 gtgcccggtc agccggcgca ccttctgttc gaagcggccg cgctcaccca ggcccatctc
     1081 cgagatccgt tgatcgagtt cgccggcgat cttgaccagc acctcgacca gaaaggctac
     1141 cgaactgcgg gtcgtgccga tgttggtcat gaacaacacg gtgttgcgcg acgttttgtt
     1201 gatctgcacc ccgtggcggt ccatcaactg atcccgcttg aacgtgtccc tgtcgtagcc
     1261 ggtgcggccg atcgacagcg tgatccgcga cgggtcgagc acaaactcgt cggcgtccca
     1321 cgccgtcatc atgttgcgca gcccggaccg cagcggctgc gcgatcgccg acgggcggaa
     1381 ttcggcggga atcaggtcgg aggtgcgcag gcacgccatg tacctgctca gcagcgggtt
     1441 gttgtcgatc gcgtcgcgca actgcatggc gttctcgatc tgccgttgga ccagttccac
     1501 gccctccagc gcgacctgac ggcggcccaa atccagtgag gcgaggatct ggtagttcgg
     1561 cgacgtccca cggtgaagac caccgcgggc ccggtgacgg tggcccggcc aaaggtgcgc
     1621 ggcaccaccg agcggttcgc ctctcagctg ttcgggaagg gggtgaccaa aacgcgttgg
     1681 agtcgctggt gatcgccggg ttcgtgcgcg gcctgtcggt ccgtgatgtg gaggccacct
     1741 tggtggaggc gctcggcgag ggggccgcgg tgtcgaaatc gacggtgtcg ggggtgtgcg
     1801 aggagatcca ggcccagttc caggcgtggt cggcgcgtcg cctcgacgac gtcgagttgg
     1861 actacctgtt cccgggcgga tcgcatttca agtatcaggc gaacgcgtcg gccgagccgg
     1921 tgctggccgc ctggggcatc gacaccgacg acaacctcaa atgcctgtgc cgacagcacc
     1981 acctactcaa aaccttttgg accggcatcg acggctggac tgatcaacag cttcccgacg
     2041 gtccggggtt ttggacgtgc ctatttattt agcgggttaa cggccgtggc cgccaccgcg
     2101 catcccaggg tcgccgccgc ccatcacagt cagtctgggt aataaacgca gctgtgctga
     2161 gtgcattgaa tcttgccggg agtgccaggt ggaagcacag gggggttgtt tacattctgg
     2221 agttcggtgc agtgaccgtc gatttctact tgaaagggat tgctgcaaac ctgggcgacg
     2281 gcgatgggtg ggccgatcat tgcggatacc gcaaaccctg cgccaaccgc cgccaagagt
     2341 gaagtgatgg ttcgtgatct tgttgggcgc atagcaccgc ctcttttcca cggttagaga
     2401 acggcacttt agcagctaaa cctgttttgc aggggcgtaa gcaaggaatg ccatgacagc
     2461 ggtgatcccg gtttgaccaa cctgtgtcct agcttgatac ccgatggcac aatcatctgg
     2521 accgcgccca caggcaagac ctacaagtcc ctgcccggca gccgactaat tcttccccgc
     2581 cttcaacacc accgccgaca ccccttcgag caccagcccc cgacgtcgcc aaagccatga
     2641 tgatgcccac acgacgacgc acccgagccg ccgaccgcgc ccgccgaatc cgcgaacaac
     2701 gcacactcaa cgccgcccac cgcgcccaat acgccgccac caacaccacc gaatgcgaca
     2761 aacccccacc caccgaacca tggaacatcg acaccaacac cgacgccggt gacgaacccc
     2821 cacccttcta acgattgcct cattgatgac tttcgtctct atatacgggc gcctcggtca
     2881 cgagagcatg gctgcaaggt aacgaaggga ttttgtgatg acgccagcgg gccgcatgga
     2941 attgacaccg caacaactgg acgcaatcgc atgggacttc ttgagatccg aattcaccgc
     3001 accgatctac gccgactggc ccatcgaccg ccgcttcgac gcgtacttgc accaccaggg
     3061 gcgcgacgac ctcgtcaatg acggggccgc ctacgacgct ctgcttcaag aagtcatggt
     3121 caacatcggt cccgcagtgc ggcgcgggct ccttggctcg cacatgaact aaggcggcat
     3181 ggacatcatc cgcaagtccc ccgaggactt gaaagtcacg ccgaacttcc tcgactacga
     3241 gcgcacccgc gctgacttcc gctggtccga cgcgccaccg ctttgcgagg gcatggggcc
     3301 gggcggttgc aacatcgcct ttgctgcggt ggatcgtcac gccgacgggc cggcggccac
     3361 gcgcaccgcg ctgcggttca ttcccgaggg gtcctgggac ggcacgctcg cctcacgtga
     3421 cctcagctat gcggagcttg ctcgcttggc caggcggttc accaacgtgc ttcgttcgct
     3481 gggaatcaac aagggggatc gggtcttcac gatcatgggg cgcaggcccg agctgtacat
     3541 cacgatgctc ggcgcgctgc gcaacgggag catcgtctcg ccgttgttct ccgcgttcgg
     3601 tccggaaccg atcgccaccc gcgtcgagat cggtgaagcc accgtgctcg tcaccaccgc
     3661 cggcatttac caccgcaaga tcgcgaagat ccgcgatcag ctgacctcgg ttcggcacat
     3721 cttcatcgtg gacggcgaca agaccaacga cgacgaccgc ggcacgctca actattggca
     3781 ctggatgaac gccgccgacg acaacgcgcc gatcgaaccc accacagccg acgacccggc
     3841 gctgctgcac ttcaccagcg gcaccaccgg cacccccaag ggcgccatcc acgtccacgg
     3901 cgcggtgacg atgcattacc tcaccggcct gtacgcactg gatctgcacg acgacgacac
     3961 gttctggtgc acggccgacc cgggatgggt caccggaacg tcctacgggg tcatcactcc
     4021 gctgttgcac ggggtcacgt cgatagtcga cgaggccgag ttcgacgccg agcggtggta
     4081 ccgcatcctg caggaccagg gcgtcaccgt ctggtatacc gcgccgaccg cgatccgcat
     4141 gctgatcaag gcgggcccag aaatcgccgc ccggtacagc ttcccgcggt tgcggttcgt
     4201 cgccagcgtc ggcgagccac tcaacgccga cgccgtctgg tggggaaagc gcgtgctggg
     4261 cttgccgatt cacgacaact ggtggcagac cgagaccggc ggcatcatgg tcgccaacac
     4321 ccccgcattc gacatcaagc cgggatcgat gggccggccg ctgccgggcg tcgacgcatt
     4381 cctggtgcac cgcaacgacg acggcaccgt caccgtgctc gacgagccgg acgtcgaagg
     4441 cgaattggcg ctgcggcccg gctggccgtc gatgttccgc ggctacctgc acgaagacga
     4501 gcgctaccgg aagtgcttcg tcgacaacct ctatctcaca ggcgatctgg caaagcgcga
     4561 cagcgacggt tactactggt tcgtcggacg cgccgacgac gtcatcaagt cgtccgggca
     4621 tctgatcggc ccgtttgagg tcgagaacgt gctgaccgag catcccgcgg tcgccgaagc
     4681 gggagtgatc ggcaagccgg accccacggt gggcgagatg gtcaaggcgt tcgtctcgct
     4741 gaaggacggc ttcaccgccg acgagacgtt acgacgtgaa ttgctcggcc acgcgcgcaa
     4801 gaagctcgga gcctcggtgg cgcccaagga gatcgagttc gtcgacgcgt tgccgcacac
     4861 ccgcagcggc aagatcatgc gacgcctgct gaaggcccgt gaactcgggc tgcccgaggg
     4921 ggacacctcg actgtcgaaa ccgcggcacc cgcgcccgac gaaaaggtga cgctatgacc
     4981 gacgccgatc tcgcccgcag cctgctgtcg gacatggtgc ggatccgacg tatggaggag
     5041 aagtgcgcgg agttgtactc cgaagccaag attcgcggct tcctgcacct ctacatcggt
     5101 gaagaggcag tcgccgcggg ttcgttgcag gcgctggcga ccgaggacgc ggtggtggcc
     5161 acctatcgcg agcatgcaca cgcgctgctg agcggcatcc cgatgacctc catcatggcc
     5221 gagatgttcg gcaagcaaga aggatgctcg cgcggccgag gcgggtcgat gcacctgttc
     5281 gacgccgacc tccgcttcta cggcggcaac gcgatcgtcg ccggtggact gccgctggcg
     5341 gtgggcttgg gcttggcgga cgcgatgttg caccgaaatc gcgtgacggc atgctacttc
     5401 ggtgacggag cggtcgccga gggtgcgttc cacgagtcgc tgaacatcgc ggcgctgtgg
     5461 cagttgccgg tgctgttctg ttgcgagaac aacctttacg cgatgggcac ggcactggag
     5521 cgggcgcagt cgcagaccga cctggcggcg aaggcgtcgg catacaaggt gtcgaccacc
     5581 gtggtcgacg ggatggacgt catcgcctgc tatgaggcgg cgcgacgcgg cgccgaggcc
     5641 gtccgcgcca ccggtggtcc gttcttcatc gagtttcgga cctaccggtt ccgggcccac
     5701 tcgatgttcg accccgagct gtatcgcgac aaggccgagg tcgagctatg gcgtcagcgc
     5761 gacccgatcc agaccttcac cgagcgatgc ctgcgtgatg gactgctcac cgaggacgac
     5821 gtcaaacaga ttgagcaggc cgcgttggac gaagtggccg acgcggtggc ctacgccgag
     5881 gccgggacat gggagagcgt cgaggatctg gaacgcgatg tcatgacacc cgtctcggag
     5941 gcggcacgat gaaaaccact taccgcaccg ctgtccacga cgggatacgt gacgcgatgc
     6001 gcgacgatcc gcgcgtcgtg ctgatgggcg aggacgtcgg ccgctacggc ggcacctacg
     6061 ccgcatcgaa gggcctgctc gaggagttcg gcccggaacg cgtgcgcgat acgccgctct
     6121 cggagctcgg cttcgtcggt gtcggaatcg gagcggcact cggcgggttg cggccgatcg
     6181 tcgaagtcat gacggtcaac ttcagcctgc tggcgctcga ccagatcgtg aataccgctg
     6241 cggcacttcg gcatatgtcc ggcggccagt tctccgtgcc gctggtcgtg cggatggcca
     6301 ccggcgccgg ccggcagctg gccgcccagc actcgcacag cctggagaac tggtacgccc
     6361 acgtgccggg gctcaaggtg gtcgcgccag cgacggtggc cgacgcgtac ggcatgctcg
     6421 gccccgccct cgccgatccg gatccggtag tgatcttcga acacgtccag ctgtacaacg
     6481 ccgcagccga cgtcgatacg ttggcaccca ccgacatctc gagcgccgcg atccggcgcg
     6541 ccggatcgga cgtcacggtg atcgcttacg gcggctgcgt gcccaaggca ctcgacgccg
     6601 ccaacgagtt gtcgctggcc ggaatcgact ccgaggtcat cgatctgcgc gtgctccgtc
     6661 ccctcgacac cgccactatc gtcgagtccg tccgcaagac tcaccgcgcc gtggtggtcg
     6721 acgaggcctg gcgcagcggc agcctggccg ccgaaatcag cgcccgcata gtcgaggacg
     6781 cgttcttcga cctcgacgcg ccgattgccc gcgtctgcac
//
LOCUS       DASWBF010000157         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_15952, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000157 DASWBF010000000
VERSION     DASWBF010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15952"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VF874_05300"
     CDS             complement(<1..470)
                     /locus_tag="VF874_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9175522.1"
                     /translation="MRVGVLGAKGKVGATMVASVEKADDLTLSAEVDAGDPLSLFVDS
                     GTEVVIDFTHPDVVMDNLKFLIDNGIHAIVGTTGFTDERLAQVESWLGDSQDTAVLIA
                     PNFAIGAVLSMRFAQEAARFFESVEVIELHHPNKADAPSGTAARTAKLIAEARKG"
     gene            complement(502..1413)
                     /locus_tag="VF874_05305"
     CDS             complement(502..1413)
                     /locus_tag="VF874_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9175523.1"
                     /translation="MKILLAGATGAIGIPLTRQLSARGHQVIGVTRRPEAAAALTAMG
                     AQPVVADVLDHGGLVHALRGISADAVIHELTALKSAPARHGGMVPTDRLRIEGTTNLL
                     VAADVVGATTFVTQSIIMGYGYRDHGDRLLTEDDSFGQPAGDANDPHIAAMLSTEQQA
                     FTAPVGIALRYGLFYGGDVEEKRAALAKRAIPVARGGLLGWIHHEDAVVATVAALERG
                     QAGNAYNIVDDRPATWKEVFTAMAQELGTPPPRNLPRWVLRLAAPYVTSFAFDTSMRV
                     SNAKAKHELGWSPKYPTYQEGIAAVAD"
     gene            1515..>1723
                     /locus_tag="VF874_05310"
     CDS             1515..>1723
                     /locus_tag="VF874_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9175524.1"
                     /translation="MAEPGYIDVHHHMLTPGVIEALTEHGVCAVGGEPLPSWSPEISA
                     VMDSAGIGRAVLSTPIPLHFLDSGAA"
BASE COUNT          266 a          568 c          582 g          307 t
ORIGIN      
        1 ccttttcgcg cctcggcgat gagcttggcg gtgcgggccg cggtgcccga cggcgcgtcg
       61 gccttgttcg ggtggtgcag ttcgatgact tcgacggact cgaagaagcg ggcggcctcc
      121 tgcgcgaacc gcatcgacag caccgcgccg atcgcgaagt tcggggcgat cagcaccgcg
      181 gtgtcctgtg agtcccctag ccaggattcg acttgcgcca gcctttcgtc ggtgaacccc
      241 gtggtgccaa cgatcgcgtg aattccgttg tctatcagga acttcaggtt gtccatcacg
      301 acgtcgggat gggtgaagtc gatgacgacc tcggtgccgc tgtcgacgaa cagcgacagc
      361 ggatcacccg cgtcgacctc ggccgaaagc gtcaggtcgt cggccttctc gacggaggcc
      421 accatcgtcg cgccgacctt gcccttggcg cccagtactc cgactcgcat gcgctgagcc
      481 tactgccgcc ggccaccggc ctcagtcggc cacggctgcg atgccctctt ggtaggtcgg
      541 gtacttcggc gaccagccaa gctcatgctt ggctttggcg ttcgacacac gcattgatgt
      601 gtcgaaggcg aacgacgtca catacggggc cgcgagtcgc agcacccagc ggggcagatt
      661 gcgtgggggc ggcgtcccga gctcctgggc catcgcggtg aagacctctt tccacgtagc
      721 gggccggtca tcgacgatgt tgtacgcgtt gccggcctgg ccgcgttcca gcgccgcaac
      781 ggtggcgacg acggcgtcct cgtgatggat ccacccgagc agtccgccgc gggcaactgg
      841 aatcgcgcgc ttggccagcg ccgcacgttt ctcttcgacg tcgccgccgt agaacagccc
      901 gtagcgcaac gcgataccga ccggcgcggt gaacgcctgc tgctcggtcg acagcatcgc
      961 cgcgatgtgc ggatcgtttg cgtcgccggc gggttgcccg aaagagtcgt cttcggtcag
     1021 cagccgatcg ccgtggtcgc ggtagccgta gcccatgatg atcgactggg tgacgaacgt
     1081 ggtcgcgccg accacgtcgg ccgccaccag cagattggtg gtgccctcga tgcggagccg
     1141 gtccgtcggc accatgccgc catgtcgtgc gggagcggac ttgagcgcgg tcagctcgtg
     1201 gatgacggcg tcggctgaaa tcccacgcag ggcgtgcacc aatcccccgt ggtcgagcac
     1261 gtcggcgact acgggttggg cacccatcgc cgtgagggcg gcggctgctt cgggacggcg
     1321 cgtcacgccg atcacctgat ggccgcgcgc gctgagttga cgagtgaggg gtatgccgat
     1381 cgctccggtg gcgccggcga gcagaatctt catgcacgcc aagctaggcc gccgggacgt
     1441 gcgcccgaat ctgtcggatg actagtcgat caacggatac gcggtcggcg ggcatctcgg
     1501 tagcgtcaat tgccgtggca gaacccggat acatcgacgt ccaccatcac atgctgaccc
     1561 ccggggtcat cgaggccctc accgagcacg gcgtgtgcgc ggttggtggc gagccgctcc
     1621 catcatggtc gccggaaatc tcggccgtga tggatagcgc cggcatcgga cgcgccgtgt
     1681 tgtcgacccc aattccgttg catttcctcg actctggcgc cgc
//
LOCUS       DASWBF010000158         6167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1201590,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000158 DASWBF010000000
VERSION     DASWBF010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201590"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..551)
                     /locus_tag="VF874_05315"
     CDS             complement(429..551)
                     /locus_tag="VF874_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175525.1"
                     /translation="MALRRLMGVPLSRDFLLSREGAGWDGDLEDIRTKRPSEFS"
     gene            719..3094
                     /locus_tag="VF874_05320"
     CDS             719..3094
                     /locus_tag="VF874_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX9175526.1"
                     /translation="MRVDGRDITVSGSLLPPVARRTNDILRVVLAAVFFIIVIAGSLI
                     TRTQWVALEQAISRIVGVLPPAQSNLIYLVYSAAIVALPFLILIGLIARHRWKLLGAY
                     AAAGLIALLSLSISGDGFSAPRWHFALSERLDTTLSQFLDDPRWIAMLAAVLTVSGPW
                     LSARWRRWWWTLLLAFVPIHLVVSAIVPARSLLGLAVGWFVGALVVLFVGTPALEVPL
                     DAAVRALGRRGFAVERLSVVRPAGHGPLVLSARSARPDSIAIVEMYGSNQRSRGALRQ
                     LWQKLKLRGSETAPLQTSMRRAVEHRALMAIAVGGLGVANTSTMAVAPLDRGWTLYAH
                     TPARGLPLSACAASTPVVRVWESLRTLHDHQISHGDLRSRQITVDDAAVRFGGFGRAE
                     YGSTDAQLQSDIAHLLVTTTDVYDAESAVAAAIDTFGNDAILTASRRLTRSAVPKRIR
                     NSVGDAGAAISAARDEVKLQTHADQIQTETITRFTRRQVIQLVLLVALVYVAYPFIST
                     VPAFFSQLRTANWWWALLGLAVSALTYIGAAAALWACADGLVSFRKLAIVQVANTFAA
                     TTTPAGVGGLALSTRFLQRAGLGAVRATAAVALQQSVQVITHVSLLIFFSAAAGASAD
                     LSHFVPSTTMLYLVAGLALGAVGAFLFVPRLRRWLGTEVRPILKEVGGDLVKLAREPK
                     RLAVIVLGCAGTTLGAALALWASIEAFGGDTTFVTVTVVTMVGGTLASAAPTPGGVGA
                     VEAALIGGLAAFGVPAAVSVPSVLLYRVLTCWLPVFVGWPIMRWLTKNEMV"
     gene            complement(3266..3487)
                     /locus_tag="VF874_05325"
     CDS             complement(3266..3487)
                     /locus_tag="VF874_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175527.1"
                     /translation="MSVNVELAKSLDKAYEDQSLKDILDASPAALAGVTDDDAAALAK
                     AFNIKTVRQLGSNKFFALAAALVALENAG"
     gene            complement(3943..5313)
                     /locus_tag="VF874_05330"
     CDS             complement(3943..5313)
                     /locus_tag="VF874_05330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9175528.1"
                     /translation="MPLADDEVFAGYTILRQLGSGGMGEVYLAQHPRLPRQEALKVLP
                     AELTLDREYRDRFDREAAIVATLWHPNIVALHDRGEFNGQLWISMDYVDGTDAARLLQ
                     GISHGLAPDEVVQIVTAVADALDYAHEHQMLHRDVKPANILLAKSHSGTRALLADFGI
                     ARRVDDPGTLTATNMTVGTVSYAAPEQLLGEVLDGRADQYALAATAFHLLTGAPPFQH
                     SNPNVVISQHLTAPPPAIDDRMPELSALGPVFSKALAKSADERYDCCLDFAKALAQQV
                     DVVASDLGAAEPTVSSDVARPAHRRPRKHRRPRKLKVRWAVAVPTILAVLLIAVSAVA
                     VAAIWRHRHPAHTASPPGTAAVPVVIVGADCATLGAAGVTTTGQQAYCARLSASNQTM
                     NMWSLYSAQEDRDDLPSGEVPAPTASPGPTDVVYPAGIEQRVEVCVQETGDTRENCQV
                     RILGQH"
BASE COUNT         1016 a         2021 c         1966 g         1164 t
ORIGIN      
        1 gatgaccaac cgcttcgcgg tctggaacca gatcattacc gccgcagcgg cttgaaagac
       61 gcagtcagcc accatctttt gccagccggt ccactcacat cgactgcgcc acaagcattc
      121 ccaaataact tgacgatgcc gtcattgatg cttcgggtcg tccttcctgc acgccgggcc
      181 gcggtagctc cctgatgcac tccggacgtc gacggtgtcg tcaattttca gcgacggcaa
      241 gccattaacc aattgctcga gcttggcatg agcatggtcg tcaaccgcgc cggccaaagt
      301 ctccatcgcc accggctcgc acatcacaac ctggccgggt ttcgtcgaca ggagcaggca
      361 cacttcacgg gcggcggatc atccggtggc ccggaaatac tccacccaga cagacgtgtc
      421 gatcaagatc aggagaactc gctcgggcgc ttggttcgga tgtcctcaag gtcgccgtcc
      481 catccggcgc cttcccgact gagcaggaag tcgcggctca gaggaacgcc catcaggcgc
      541 cgcagtgcca cgggccagga ctccgtcgaa ggctggctgg acgtcgcggc gaggtccctg
      601 ctgtacttcg ccgcgcgtgg agggaaacgc tcatcggcgt ctgaaggcgt gggacatggt
      661 tgtcggcgcg ccgctgatac gcgccgccga agcgtcgaga cgaaataccg taccggtcat
      721 gcgggtcgac ggacgggaca tcactgtctc cggcagcttg ctgccgcctg tcgcccgacg
      781 gaccaacgac atcctgcggg tcgtcctcgc ggcggtgttc ttcatcatcg tcatcgcggg
      841 ttcgctcatc acccgcacgc agtgggttgc cctcgagcaa gccatctcgc gaatcgtcgg
      901 cgtgctgccg ccagcgcagt cgaatctaat ctatctggtc tacagcgccg ccattgtcgc
      961 cctaccgttt ctgatcctga tcggcctgat cgcccgacac cggtggaagc ttctcggggc
     1021 gtacgcggcc gccgggctta tcgcgctcct ctcgctgtcg atcagcggcg acggcttctc
     1081 cgcgccgcgc tggcacttcg ccttatccga gcgcctcgac accacgttgt cgcagttcct
     1141 cgatgacccc cgctggatcg cgatgctcgc tgccgtgctg accgtctcgg gcccgtggct
     1201 gtccgcgcga tggcggcgct ggtggtggac gctactgctg gcgttcgttc cgatccactt
     1261 ggtcgtcagc gcgatcgttc ccgcccgttc actgctcggc ctggccgtcg gctggttcgt
     1321 cggcgccttg gtggtactgt tcgtcggcac cccggcgttg gaggtgcccc tcgacgccgc
     1381 cgtccgtgcg ctgggtcggc gcggatttgc ggtggagcgc ctgagcgttg tgcggcccgc
     1441 cggccacgga ccgctcgtgt tgtccgccag atcggcgcga ccagattcga ttgcgatcgt
     1501 cgagatgtac ggctcgaacc agcgcagccg cggggcgctg cgccagctct ggcagaagct
     1561 caaactgcgc ggcagcgaaa ccgcgccgct gcagacctcc atgcgccgcg ccgtcgagca
     1621 ccgcgcactc atggccatcg ccgtcggcgg actcggcgtc gccaacacat cgacaatggc
     1681 tgtcgctccc ctcgaccggg gatggacgtt gtacgcgcac accccggcgc gcgggctccc
     1741 gctcagcgcc tgcgccgcat cgacacccgt tgtgcgcgtg tgggaatcgc tgcggacact
     1801 gcacgaccat cagatctccc acggcgatct gcgcagcagg caaatcaccg ttgacgacgc
     1861 cgccgtgcgc ttcggcggct tcggccgcgc cgagtacggc agcaccgacg cccagctgca
     1921 atcggacatc gcccacctcc tggtgacgac gaccgatgtg tacgacgcgg agtccgccgt
     1981 ggccgcggcg atcgatacct tcggcaacga tgccattctg accgcatcac gccgcctcac
     2041 cagatcggct gtgcccaaac gaatccgcaa ttccgtcggt gacgccggcg ccgccatctc
     2101 cgccgcccgc gacgaggtca agctgcagac acatgccgac cagatccaga ccgagacgat
     2161 tacccgtttc acgcgccgac aagtcattca actggtgttg cttgtcgcgc tggtgtacgt
     2221 cgcctatccg ttcatcagca cagtaccggc gttcttttca cagctacgga cggccaactg
     2281 gtggtgggca cttctcgggc tggcggtgtc ggcgttgacg tacatcggtg cggcagccgc
     2341 gctgtgggcc tgcgccgacg ggttagtgag cttcagaaag ctcgcaatag ttcaggtagc
     2401 caacacgttt gctgcgacca ccacgccagc aggcgtcggc ggtctcgcgc taagtacgcg
     2461 attcctgcaa agagccggcc ttggcgccgt acgcgccacc gcggccgtgg cgctgcagca
     2521 gtcggttcag gtgatcacgc acgtttcgct gttgatcttc ttcagcgcgg ccgcgggtgc
     2581 gtcggccgat ctgtcgcatt tcgtcccgtc gaccacgatg ctgtatctgg ttgccgggct
     2641 ggcgctcggg gccgtcgggg cgttcctgtt cgtcccccgg ctgcgccgct ggctcggcac
     2701 cgaggtacgc ccgatactca aggaggtcgg cggtgacctc gtcaaactgg cccgcgaacc
     2761 caagcggctc gccgtgatcg tattgggctg tgcgggaacg actctgggcg cggcgctggc
     2821 gttatgggcg agcatcgagg cgttcggcgg cgacaccacg ttcgtcaccg tcacagtcgt
     2881 caccatggtc ggcggaactc tggcgtcagc ggcacccact cccggtgggg tcggtgccgt
     2941 cgaagcagca ctgatcggtg gactcgcggc gtttggtgta ccggcggccg tgagtgtgcc
     3001 gtcggtgctg ttgtaccggg tgctgacctg ctggctgccg gtgttcgtcg gctggccgat
     3061 catgcgatgg ttgaccaaga acgagatggt ctagtcgcgc tcaggcggtt gctgacccgc
     3121 gacttgtcaa caatcggaaa ccgcgacatc ggatccggtg ttcttcagac accgtcaagt
     3181 gtgtgtgagt ccagcacgtc acggtgccga cagaagccgc cggagcggcc ttcggcctca
     3241 gccgcgccgg tcggctcgac gcgcgttatc cggcgttctc caacgccacc agcgccgccg
     3301 cgagcgcgaa gaacttgttg gacccgagct gacggaccgt cttgatgttg aacgccttcg
     3361 ccagcgcggc ggcatcgtca tcggtcaccc cggcgagcgc cgccggtgac gcgtcgagga
     3421 tgtccttcag tgactggtcc tcgtacgcct tatcgagcga cttcgcgagt tcgacgttga
     3481 cagacatggg cctctccttc gattactggc tctacaacat gtctcgatcc tgctccagtg
     3541 gacatttccg gttcaagtgc gtggcccgta gaggcccgcg actgggccga gatccgtcgt
     3601 cgtcgccgct agtacccccc gatgccgatg tggtcgcggg cgctcggttt gtagccgatc
     3661 gcggtgttca cgtggcactg tcaacttgag caatccgagg gcgattttcg ccggttcacc
     3721 tccgcgattc accaggtcgc gtgtgacgta tgaggcttat gtggttagca gaacgtccac
     3781 acgacctcaa gttgacagtg cccccgtccc tactttgacc aggtctttta aggtggggcg
     3841 ggcgagactt gaacccgcga cctacggatt atgagtccct atgcggccac ccaccggttg
     3901 cagccgatcg cttaccgcta tcggacaggg cgttgatcag gatcagtgtt gccctaagat
     3961 gcgcacctgg cagttttcgc gggtgtcccc tgtctcctgt acgcaaacct cgacacgctg
     4021 ttcgataccc gccgggtaga ccacgtccgt tggacccgga ctggccgtcg gtgccgggac
     4081 ttcaccagac ggcaggtcgt cgcgatcttc ctgggcactg tacagcgacc acatattcat
     4141 agtctggttg ctcgcagaca gccgtgcgca gtaggcctgt tgaccggttg tggttacgcc
     4201 tgcggcaccc agggttgcac agtcggcgcc gacgatcacc acgggcacgg ccgcggtgcc
     4261 cggtggggac gcagtatgag cgggatgccg gtgacgccag atggcagcga cggcgacggc
     4321 tgacaccgcg atgagcaaca ccgcaaggat cgtcggcacg gcgactgccc agcgcacttt
     4381 cagtttcctt ggtcgtcgat gcttccttgg tcgtcgatgc gcgggcctgg cgacgtccga
     4441 ggacaccgtg ggctcggcgg caccgagatc ggaggcgacg acgtcgactt gctgcgccag
     4501 cgccttagcg aagtccaggc agcagtcgta gcgctcgtca gcagacttgg caagcgcctt
     4561 ggagaacacc ggcccgagtg cagacaactc cggcatgcga tcgtcgatcg cgggcggcgg
     4621 cgcggtcagg tgctggctga tgacgacgtt ggggttggaa tgctgaaacg gcggcgctcc
     4681 ggtcaacaaa tggaaagcgg tggcggccaa ggcatactgg tcagcgcgac cgtcgagaac
     4741 ctcgcctagg agctgctcgg gcgccgcgta ggacaccgtg cccaccgtca tgttggtggc
     4801 ggtgagggtg ccggggtcgt cgactcggcg ggcgatgccg aagtcggcca gcagcgcgcg
     4861 tgtcccggaa tgcgattttg ccagcaagat gttggccggc ttgacatccc ggtgcaacat
     4921 ctggtgctcg tgcgcatagt cgagcgcgtc ggcgaccgcg gtcacgatct gcacgacttc
     4981 gtccggcgcc agaccatggg agatgccctg caggaggcgg gcggcgtcgg tgccgtccac
     5041 gtagtccatc gatatccaca gttggccgtt gaactcgccg cgatcatgca gtgccacgat
     5101 gttcgggtgc cacagggtcg cgacgatcgc ggcctcgcgg tcgaaccggt cacggtattc
     5161 ccggtcgagc gtgagctccg cgggcagcac cttgagggct tcctgccggg gcaacctcgg
     5221 gtgttgagcg aggtacacct cacccatccc gcccgatccc agctggcgca gaatcgtata
     5281 tccggcaaat acttcatcgt cagccagcgg catcgccata tcctactgtc ggcgccaatt
     5341 ggtcgacgat ccgaacgccg gttcctgcgc caccacgggc ggcggtacga gcagcactcg
     5401 ccggtgaact atgtgaatcc gttgcataca cccgtactgg tcatgaacgg tgtggccgat
     5461 ccacgggtgc cggtctcaaa gggcgaggag gctctacaac gggctgcgct tcgagggccg
     5521 cgaggtgacg atgatgcgat acccgcgtga gccgcatatc tttcaccgag ctggaacatc
     5581 aggtcgactc aatctcgcgg atattgaact ggtacgactc acatttgccg aactgatccg
     5641 aactcttctg aatcccttga cagcttccgt gatggaacgt gtagctcaca gggcgtactc
     5701 gacaatggtg acgagcgccg cgccgaggat tgcgaaagtg agcacggcca tcgcggcctc
     5761 cttcagcgcc gtgcgctcag gaccgcatcg taaagctcgc gacgcgacgg cgccccggga
     5821 ctcgccgcgc cgacagttag ctcgcggcgc gccgggtcgc cagttcgcgt acacgccgcg
     5881 tacacgaact gccccgcgac aacttgccgc cacagttggt aacgacgaat tcgttatttc
     5941 gggagcgttt gtacaacgaa tggccaggcc ttgaggagat ctagaaagga ccgctgaagt
     6001 gcagcaatgc ggtgattaaa catcggccgg aatgggcttg acgcccttgc ggactatccg
     6061 ctgactctcc catcatgcga ggcatgggtc taaccatccg gctacccgcc ctgctgcggc
     6121 agtcgcgtcc gcggccgcga tcgctgccag cgcgtcagca aggtttc
//
LOCUS       DASWBF010000159         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_938204,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000159 DASWBF010000000
VERSION     DASWBF010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938204"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VF874_05335"
     CDS             <1..1085
                     /locus_tag="VF874_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9175529.1"
                     /translation="LAGKKSLDDFTVHDSAWYRESSVDLRLGTAVSAIGRSAHTVTLA
                     DDSTVAYDKLVLATGSRSRRPRIPGADAAGVHYLRTFDDAGELNSVLREGSSLAIVGA
                     GWIGLEVASGARARGVDVTVVETARLPLLAALGAEAAEVFAALHREHGVNLLLETNVA
                     EITVQSGEATGLKLDNGSTIASDTVLVAVGAAANIELAEQSGLDVGDGGVVVDATLRS
                     SDPDIFAVGDIAAAEHPLFKTRIRTEHWANALKQPVVAAANMLGRRAEYDELPYFFTD
                     QYDLGMEYVGHAPSYERVVFRGDVGGREFIAFWVDDQDRVLAGMNVNVWDVVDDLKAL
                     IRSRAAVSAEQLADKACPLSELADLS"
     gene            complement(1390..1665)
                     /locus_tag="VF874_05340"
     CDS             complement(1390..1665)
                     /locus_tag="VF874_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175530.1"
                     /translation="MIARRTSGANMVGWFVGSLLPWVACWSAPVSLWWLVPCGAVAIG
                     YAFWLYRSVARSDRLQHQGIPARGVVLEVVRPLMNVVINKRLHQADA"
     gene            complement(1662..1952)
                     /locus_tag="VF874_05345"
     CDS             complement(1662..1952)
                     /locus_tag="VF874_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175531.1"
                     /translation="MPTDRIGPSTHATSAHPDGTDTGPQTLGVDTTARYRDAVRLRSD
                     LRLLLEAWCVLVLAPAVVATMVVMGGRGSEDAGGGVVLTRSSATLCNSGFSW"
     gene            complement(1955..2191)
                     /locus_tag="VF874_05350"
     CDS             complement(1955..2191)
                     /locus_tag="VF874_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175532.1"
                     /translation="MVSAAGGFSPVFTTTAFAQAEHSAAIATNTANRRSNNSSRSACE
                     SASLSSASSLFNAASMASHSASAWSNATELIPAM"
     gene            complement(2366..2527)
                     /locus_tag="VF874_05355"
                     /pseudo
     CDS             complement(2366..2527)
                     /locus_tag="VF874_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          425 a          826 c          844 g          432 t
ORIGIN      
        1 ttctcgccgg caaaaagtcg ctcgatgact tcaccgtgca cgactccgcg tggtaccggg
       61 aatccagcgt cgacctgcgc cttggcaccg ccgtttcagc gatcggccgc tccgctcaca
      121 cggtcacgtt ggctgacgac agcaccgtgg cctatgacaa gttggtgctg gcgacgggtt
      181 cgcgctcgcg gcgcccacgc atccccggtg ccgacgccgc cggcgtgcat tacctgcgta
      241 cgttcgatga tgccggtgag ttgaattccg tgctgcgcga gggttcttcg ctggcaatcg
      301 tcggcgctgg atggatcgga ctggaggtag cctcaggtgc ccgcgccaga ggcgtcgacg
      361 tcaccgtcgt cgagaccgcg cggctgccgc tgctcgccgc actcggcgcc gaggccgcgg
      421 aggttttcgc agcgttgcac cgagaacatg gggtgaactt gttgctcgag accaacgtcg
      481 ccgagatcac ggtccagtcc ggcgaggcga ccggactgaa gctcgacaac ggatccacga
      541 tcgcgtccga cactgtgctc gtcgcggtcg gcgccgccgc caacatcgaa ctcgcggagc
      601 agtcgggcct ggatgtcggc gatggcggcg tggtcgtcga cgccacattg cgcagcagtg
      661 acccggacat cttcgccgtc ggcgacatcg ccgcggccga gcacccgttg ttcaagaccc
      721 gcatccgcac cgagcactgg gccaacgcgc tcaagcagcc cgtggtcgcg gccgcgaaca
      781 tgctgggccg gcgcgccgaa tatgacgagc tgccttactt tttcaccgac cagtacgacc
      841 tcggcatgga gtacgttggt catgcgccct cctatgagcg agtggtgttc agaggtgacg
      901 tgggcggccg cgaattcatc gcgttctggg ttgacgacca ggatcgcgtg ttggccggga
      961 tgaacgtcaa cgtgtgggac gtggtcgacg acctaaaggc gctgatccga tcacgggccg
     1021 ccgtctcggc tgagcagttg gcggacaaag cctgcccgtt gagcgagctc gccgacttgt
     1081 cctgagcgcg gctcagccgc cgagaatacg acgcttggcc gcgacgaact cgtcatcggt
     1141 gagtgcccct gcgcggtgca attcggcgac ctcccgcagc tgatcggcga tgtcactcca
     1201 ggcgtcgtga cgggttcgtc gtcgtaggat gtgtcgctgc tgtcgcggct gacctcgaag
     1261 tgctccggcc tttcgggatc gacgcgtaat gccaatgccg aaccaaccga ggggatttcg
     1321 ccgaacatga agatgccccg ggatttggcc tcataccgtg gagcgccatc ggaccgttcg
     1381 atgcgcagct taagcgtccg cttgatgtag gcgtttgttg atcaccacgt tcatcagcgg
     1441 cctgacgact tccagtacca ctcccctggc cgggatgccc tggtgctgca gccggtcact
     1501 gcgggccacc gatcggtaca gccagaacgc gtatccgatg gcgaccgctc cgcacggcac
     1561 cagccaccac agcgacaccg gcgccgacca gcacgccacc cacggcagca acgaccctac
     1621 aaaccagccg accatgttgg cgcccgacgt tcggcgtgcg atcaccagga aaaccccgaa
     1681 ttgcacaagg tagccgacga ccgggtcaac acgacgccgc caccggcgtc ctcggatccg
     1741 cggccaccca tcacgaccat ggtggcgacc accgccggtg cgagcaccag cacgcaccac
     1801 gcctcgagca gcagccggag gtcgctgcgc aggcgcaccg cgtcacgata gcgcgcggta
     1861 gtgtcgacac ccagtgtctg cgggccggtg tcggtgccgt cgggatgagc ggaggtcgca
     1921 tgggttgaag gcccgattct gtcggtgggc atggctacat tgcgggtatg agttcggtgg
     1981 cgttcgacca ggcggaggcg ctatgcgagg ccatcgatgc tgcgttgaac aagctcgacg
     2041 cgctggacaa ggatgcgctc tcgcacgccg agcggctgga gttgttggag cgccggttcg
     2101 ctgtgttggt ggcgatggcc gcgctgtgct cggcctgtgc gaacgccgtg gtggtgaaca
     2161 ccggcgagaa gccgccggcc gccgagacca cgttctccac ctcgccggtc ggtcggtccg
     2221 accccaatct ggttgacgcg tcggactatt acgtcaccac agacggtctc aaggggttac
     2281 tacttcacga ccccgagcgg caagtggaat tgcgctattc tgccgcgtac cgaagtgggc
     2341 tgccaggccg cggccggcac gcagcgccgt tggacagccc gcggttgatg gcggcgtcga
     2401 tcccggcgcg gtgcttgcgg atggtgcgcg cggctttgac gaactccggg atacggcaac
     2461 gctgagcgcg agaacaccac cgatcgagca gctcgccggc ggtggctggg tcgaggtcac
     2521 cggcgaa
//
LOCUS       DASWBF010000160         3749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_739983,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000160 DASWBF010000000
VERSION     DASWBF010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739983"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VF874_05360"
     CDS             complement(<1..839)
                     /locus_tag="VF874_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX9175533.1"
                     /translation="MESFIHLRKGTTPRRLHADLDGLKDDELGRGGFTGRTANMYRRH
                     DPTAYRAEGPLRPLDVLSSELKPSDATDAAGGPLLVFSNPDCRVSLSRRAEEMPFHTR
                     HVDGDLLCFVHRGTGLLETEFGPLPYREGDWVYIPKACTWRQVPEGETTLLMVEATDE
                     FRVPPPGQLGRHFPFDPSQVVIPEPVPLADPPPSGGDEYEVRLVHEGGPTTLYYQHNP
                     IDVEGWRGDNFAFTSNIADYNVVTSDSVHLPPTVHLFMQATGVYVMNFLPKPAESVPG
                     TERT"
     gene            980..1543
                     /locus_tag="VF874_05365"
     CDS             980..1543
                     /locus_tag="VF874_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175534.1"
                     /translation="MHSDEYISYEQFGRDFFEVAVTEERVAAAFAVIAGDEFEMGPMR
                     QGPGGIAKVTAFVTIKQPKAHRHLGDTITFDIRIPLAIELIIDLKLDKPRFNVAGEIK
                     LHAEARAVEPLLIYIDVTKPRPSDITVDVASRSLRGELVRFVAGVDAEIKRFIAKYVR
                     EEIDKPASKKAQVIDVAQRLDEAWTGV"
     gene            complement(1547..2245)
                     /locus_tag="VF874_05370"
     CDS             complement(1547..2245)
                     /locus_tag="VF874_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9175535.1"
                     /translation="MPARDARLAESVLARAGILQGVSPSAAADLIRQLHVVEFPRGHT
                     IYCTGDPGDRLYIVISGKVKIGRRSPDGREKLLTIMGPSDVFGVLSMFDPGPRTATAT
                     AITTVCAAAMDRDALASWIGSQPEITERLLRVLARRLRRTNDQLTDLIFTDVPGRLAK
                     ELLKMAQKFGTTEREGMRVTHDLTQEEIAQLVGASRETVNKALSDFAHRGWIRWDGKS
                     VLILDTERLARRAR"
     gene            complement(2333..3547)
                     /locus_tag="VF874_05375"
     CDS             complement(2333..3547)
                     /locus_tag="VF874_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9175536.1"
                     /translation="MAINLELPRKLQAVVDMAHQGAAEMLRPISRKWDLREHEYPVEL
                     DTLADLFEGISEANAIAFAGAEAFRDADEAKNENHNGGNMSAVVNVIEVSWGDVALML
                     SVPRQGLGNAAISGVATDEQLERLGNGVWAAMAITEPGFGSDSAAVSTTAKLDGDEYV
                     INGEKIFVTAGSRATHIVVWATLDKSRGRAAIKSFIVPRDHPGVTVERLEDKLGIKAS
                     DTAVIRFDNVRIPKENLLGSPDIDVEKAFAGVMETFDNTRPVVAAMAVGIGRAALEEL
                     RKILTEAGIEISYDKPAHSQSAAAAEFLRLEADWEAGYLLTVRSAWQADNDIPNSKEA
                     SMGKAKAARVASDITLKAVELAGTTGYSEQTLLEKWARDSKILDIFEGTQQIQQLVVA
                     RRLLGLSSSELR"
     gene            complement(3578..>3749)
                     /locus_tag="VF874_05380"
     CDS             complement(3578..>3749)
                     /locus_tag="VF874_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9175537.1"
                     /translation="AREAALAKRLGTDKGMQIGLDGVQLLGGHGYTKEHPVERWYRDL
                     RVIGVAEGVVVI"
BASE COUNT          605 a         1237 c         1228 g          679 t
ORIGIN      
        1 gtgcgctcgg tgccgggcac actctcggcg ggcttgggca ggaagttcat cacgtacacg
       61 ccggtcgctt gcatgaacag atgcacggtc ggcggcaggt gcacgctgtc ggatgtcact
      121 acgttgtagt cggcgatgtt ggaggtgaac gcgaagttgt ccccgcgcca gccctcgacg
      181 tcgatcgggt tgtgttggta gtaaagcgtt gtcggcccgc cctcgtggac cagccgaacc
      241 tcgtactcgt cgccgccaga aggaggtggg tcggccagcg gtacgggctc ggggatgacg
      301 acttgcgacg ggtcgaacgg gaagtgccgg ccgagctgcc ccggtggcgg cacccggaac
      361 tcgtccgtgg cctcgaccat caacagcgtc gtctcaccct ctggaacctg acgccacgtg
      421 caggccttcg ggatgtacac ccagtcgccc tctcggtagg gcagcggccc gaactcggtt
      481 tcgagcaggc cggtgccgcg gtgcacgaag cacagcaggt cgccgtcgac atggcgggtg
      541 tggaacggca tctcctcggc gcgacggctc agtgacaccc tgcagtcggg gttggagaac
      601 accagcagcg ggccgccggc cgcgtcggtg gcgtcgctgg gcttcagctc actcgacagc
      661 acatcgaggg gccgcagcgg accctcggcg cgataggcgg tggggtcgtg gcggcgatac
      721 atgttcgccg tacgtccggt gaagccgccg cggccgagtt cgtcgtcctt gagaccgtcg
      781 aggtcggcgt gcagtcgccg cggtgtggtg cctttacgca ggtggataaa cgattccatc
      841 gtcggctcct cggatcggcc ggactgaaac taaaagtgac attacttttc gttccacgcg
      901 cgcacaaggc cgtttcggcg tgtcgcgaag ctgacttcac tgtcggcgcc ggacaacgcg
      961 tgggaaggta gtcagcgaca tgcacagcga cgagtacatc tcctacgagc agttcggccg
     1021 cgacttcttc gaggtggctg tgaccgaaga gcgggttgcc gccgcgtttg ccgtaatcgc
     1081 cggcgacgag ttcgagatgg ggccgatgcg ccaaggcccc ggcggtatcg cgaaggtcac
     1141 cgccttcgtc acgatcaagc agcccaaggc gcaccggcac ctcggcgaca cgatcacgtt
     1201 cgacatccgc atcccgctgg cgatcgaact gatcatcgat ctcaagttgg acaagccgag
     1261 attcaacgtc gctggtgaga tcaagctgca cgccgaggcg cgcgcggtcg agccgctgct
     1321 gatctacatc gacgtcacca agccccgccc ctcggacatc acggtcgacg tggcgtccag
     1381 gtcgttgcgc ggcgagctgg tgcgcttcgt cgccggcgtc gacgccgaga tcaagcggtt
     1441 catcgccaag tacgtccggg aggagatcga caagccggcg tcgaagaagg cgcaagtcat
     1501 cgacgtggcc cagcgcctcg acgaggcctg gaccggcgtc tagccgttag cgggcgcggc
     1561 gagccaaccg ctcggtgtcc agaatcagca cgctcttgcc gtcccagcgg atccagccgc
     1621 ggtgcgcgaa gtcggacagc gccttgttca cggtttcccg ggatgctccg accagctgtg
     1681 cgatctcctc ctgcgtgaga tcgtgcgtga cgcgcatccc ctctcgctcc gtcgttccga
     1741 acttctgtgc catcttgagc agttctttcg cgagtcggcc cggcacgtcg gtgaagatca
     1801 gatcggtcag ctggtcgttg gtgcgacgca gccggcgggc gagaacccgg agcagccgct
     1861 cggtgatttc gggctggctg ccgatccacg aggccagcgc atctcgatcc atcgccgccg
     1921 cgcacactgt ggtgatggct gtcgcggtcg cggtgcgcgg tcccgggtcg aacatcgaca
     1981 acacgccgaa cacgtcggac ggacccatga tggtcagcaa cttctcgcgg ccgtccggcg
     2041 agcgccggcc gattttcacc ttcccggaga tcacgatgta cagccgatct ccggggtcac
     2101 cggtgcagta gatggtgtga ccgcgcggga actcgacgac atgtagttgt cggatcaggt
     2161 ccgccgcggc agagggcgaa acgccctgca ggatgccggc gcgggccagc acagactctg
     2221 cgagtcgggc atcgcgggcg ggcaccgcga ccccagaggc atgattgttc acgtcggctc
     2281 cctgtttttg atgatgtccg acatacccag gccacgaggg gttaaaccgc gatcacctca
     2341 gttcggacga cgacagccca aggagccgcc gcgccaccac cagctgctgg atctgctgcg
     2401 tgccctcgaa gatgtcgagg atcttggaat cgcgcgccca cttctccagc agggtctgct
     2461 cggaatagcc tgtggtgccg gccaattcga cggccttcag cgtgatgtcg ctggccaccc
     2521 gcgccgcctt ggccttgccc atcgacgctt ccttgctgtt ggggatgtcg ttgtcggcct
     2581 gccacgccga gcgcacggtc agcagataac cggcttccca gtcggcctcc aacctcaaga
     2641 actccgcggc ggccgcgctc tgcgaatgcg ccggcttgtc gtaggagatc tcgatgccgg
     2701 cctcggtgag gatcttgcgc aactcctcga gggccgcgcg gccgatcccg acggccatcg
     2761 cggccaccac cgggcgggtg ttgtcgaagg tctccatcac cccggcgaag gccttctcga
     2821 cgtcgatgtc cgggctgccg agcaggttct ccttcgggat gcggacgttg tcgaaccgga
     2881 tcaccgcggt gtcggaggcc ttgatgccga gcttgtcctc gagccgctcg acggtgacac
     2941 ccgggtggtc acgcggcacg atgaacgact tgatcgcggc gcggcccctc gacttgtcca
     3001 gcgtcgccca caccacgatg tgggtggcgc gtgatcccgc ggtcacgaag atcttttcgc
     3061 cgttgatgac gtactcgtcg ccgtcgagct tggccgtcgt cgacacggcg gcggagtccg
     3121 agccgaagcc cggttcggtg atcgccatcg cggcccacac gccgttgccc agccgctcca
     3181 gctgctcgtc ggtggccacg ccgctgatcg cggcattgcc gaggccttgg cgcggcaccg
     3241 acagcatcaa cgcgacgtca ccccaactga cttcaattac gttgacgacc gccgacatgt
     3301 tgccgccgtt gtggttctcg ttcttggcct cgtcggcgtc gcggaacgct tccgctccgg
     3361 cgaacgcgat ggcgtttgcc tccgagatcc cttcgaaaag atcggccagc gtgtcgagct
     3421 cgaccgggta ctcgtgctcg cgcagatccc atttgcgcga aatcggccgc agcatctcgg
     3481 ccgcaccctg gtgcgccatg tcgaccaccg cctgcagctt gcgcggcagc tccaaattga
     3541 ttgccatggt tggactttcc ttagatgacg actcggttta aataactaca acgccctcgg
     3601 cgacgccgat cacgcggagg tcgcggtacc agcgttcgac gggatgttcc ttggtgtagc
     3661 cgtggccgcc cagcagttgc acgccgtcga gcccgatctg catgcccttg tcggtgccga
     3721 gccgcttggc cagcgccgcc tcccgagcg
//
LOCUS       DASWBF010000161         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_601687,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000161 DASWBF010000000
VERSION     DASWBF010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601687"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1930)
                     /locus_tag="VF874_05385"
     CDS             complement(212..1930)
                     /locus_tag="VF874_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9175538.1"
                     /translation="MTEITGGELFARALQAEGIEFLFGLPSPEIDPLLAQLDAHGIRL
                     VPVRHEAAGVHMAEGLYKTTGKVAAVLGNPGPGSANLLPGVVTALHEGVPVLVITSQH
                     RLGIVYPSPPSTFQGQDQLDLFRPAVKWGGPIFEWSRIPEVLRLAFREMHNGRPGPVH
                     VELPAPILYETGDPQTAPVLPPAAYRIGALPPSDRQLDEAAALLVAAERPVLIAGQGV
                     DRAGARDLVIEIAELLNCPAMSSMAGRASVPADHPNYVSGFGPAGDLARSEADVLLVA
                     GSRMGNLDVPYDTYWGDPEGKRLIQIDIDPRHFGVTRPLHLGILADARAALDGIAVRM
                     RTMNVGTRDGADLARYRQLDEQVRAQLAAPILEWPGPGIHPAHAAAAIGAVFGPDAVY
                     TADGGNTSLWAYNTLPPTQPRSYHSILELGMLGTGIPSAIGAKLGAPDREVVCVTGDG
                     AAGFNFMELQTAAREGLDITVIVFAEGSWTMEEVNELQLYGTTFGTAQGEIRWDVVAQ
                     GLGCHGEYVERIEDLDGALQRSREHKGPSLVCVRTDHAANLAVPAEMMARFFEVYSGP
                     AKPATV"
     gene            2132..2938
                     /locus_tag="VF874_05390"
     CDS             2132..2938
                     /locus_tag="VF874_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175539.1"
                     /translation="MRSPVLVGGAGTSGEAVVFVHGNPDTGSDWLPLMEPVAEFATAV
                     APDMPGFGGADKPEHWDYTVAGYASHLGGLLDQLGIERVHVVAHDFGGPFALKWAADN
                     GEKLASVTLINTGVLLGYRWHRLARIWRTPVLGEVFMLTTSPRLARFVVHHDNPGLPE
                     EWVQKIADHARPPETKRAVLRLYRSTPPSDMELVAGPLRERDPDTLVVWGDADVYIPA
                     VQAQRQREVFSRARIEILPGVGHWAWLEQPERVADLVVPFLRERVGGTAQ"
     gene            2962..3342
                     /locus_tag="VF874_05395"
     CDS             2962..3342
                     /locus_tag="VF874_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX9175540.1"
                     /translation="MSLLEPTPEQFAALAARPADAAVVMVNLLKFNQPGGLERYLQYG
                     QEVAPHVERVGGKVRYGGAAPTVVIGDGERPWWDAILVVEYPSPAAFIEMVTDDGYLQ
                     VHQHRVAALERGDLIATSTWMPAD"
     gene            3390..4292
                     /locus_tag="VF874_05400"
     CDS             3390..4292
                     /locus_tag="VF874_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX9175541.1"
                     /translation="MYLITGAAGKVGGVSRSVVEILIGKGESVRAMVHREDSRAQRLR
                     ELGAEVVIGDLTNPRDVVHAMTGVERMFFNMSVSLDYLEATAIVCAAAREVGGYEVIV
                     NMSQMTVSEMTLTSTGESFQQWLHWLSEQVMNWSGVPVVHIRPTIFLDNPLFTMLARR
                     SVHDRGVLPLPFGTGRTSPIAAADVARLIAAVLLDPADRIGDVYELTGPEVLGINGLA
                     EHYSRALNRSVAAEDIAYDDWIRQYLRASGLPDHVQQHIATMARLHHEDHYNRVSDDF
                     QRVIGEPAQTVEQYIAAHPQLFGG"
     gene            4411..4947
                     /locus_tag="VF874_05405"
     CDS             4411..4947
                     /locus_tag="VF874_05405"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX9175542.1"
                     /translation="MAGYRDLEIARRDFAKLADEVERERFLVKRAALLTKDENGNPTV
                     VELGNHLGAKGAGFGAGIGILVGLFAPPILGSVLIGAAAGALVAAFAGHEMKVGLRHE
                     VSRALDAGTAAVVALVPPSSLTFAERDLLGADSKSIVRLDKSTVNSLEKEIERALHEH
                     QPAQPGSDASTGSVGTSS"
     gene            complement(4916..>5395)
                     /locus_tag="VF874_05410"
     CDS             complement(4916..>5395)
                     /locus_tag="VF874_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX9175543.1"
                     /translation="DIQAAGIDLGQLEILARDDDMSVVAHLGPDLLGADWDPARAAAN
                     LTADPDRPIAAALLDQRVMAGVGNVYCNELCFVFGVLPTAPVARVADPLRVVSRAHDM
                     MVANRARWNRTTTGDRRHGNELWVYGRPGQPCRRCGTLIETDWSGERVSYWCPRCQC"
BASE COUNT          940 a         1748 c         1810 g          897 t
ORIGIN      
        1 cgaactcata cccgcaatgt atccacgccc accgacacgt tcgggcctca aaccctgccg
       61 gacaacgcaa ttcaggccca gttatccaca accacgaatt catccacaga tttcgacgaa
      121 ctgccgaatc agccttgaca aaccgaggct gccgagttac gtgacgtagc gctcaaccaa
      181 tgacggaccc agccgtcgtc cggcagcgtc cctagacggt cgccggcttc gccggtccgg
      241 agtagacctc gaagaagcgc gccatcatct cggccggcac cgcaaggttc gcggcgtggt
      301 cggtgcgcac gcagacgaga ctggggccct tgtgctcgcg cgaccgctgc agtgcgccgt
      361 cgagatcctc gatccgctct acgtactcgc cgtggcagcc aagtccctgc gcgacgacat
      421 cccagcggat ttcgccctga gcggttccga acgtcgtgcc gtacagctgc agctcgttga
      481 cttcctccat tgtccacgag ccctcagcga acacgatcac ggtgatgtcg aggccctcgc
      541 gtgcggcggt ctgcagctcc atgaagttga agcccgcggc gccgtcgccc gtgacgcaga
      601 cgacctcacg gtcgggcgcg ccgagcttag cgccgatcgc cgacgggatt ccggtaccca
      661 gcatcccaag ctcgaggatc gagtgatacg accgcggttg cgtcggcggc aacgtgttgt
      721 aggcccacag tgaggtgttg cctccgtcgg ccgtgtacac cgcgtccgga ccgaacaccg
      781 cgccaatggc cgcggccgcg tgcgccggat ggatgcccgg gcccggccac tcgaggatcg
      841 gtgcggccag ctgggcccgt acctgctcat cgagctggcg gtagcgtgcc aggtccgcgc
      901 cgtcgcgcgt gccgacattc atcgtgcgca tccgcaccgc gataccgtcg agagcggccc
      961 gcgcgtcggc gaggattccg aggtgcagtg gccgcgtgac gccgaagtgg cgcgggtcga
     1021 tgtcgatctg gatcagccgc ttgccttccg gatcgcccca gtaggtgtcg tacggaacgt
     1081 cgagatttcc catccgtgaa ccggcgacga gcaacacgtc ggcctcgctg cgcgcgaggt
     1141 cacctgcggg cccgaacccg gagacgtagt tggggtgatc ggccgggaca ctcgcacggc
     1201 cggccatcga gctcatcgca gggcagttca gcaactcggc aatctcgatg accagatcgc
     1261 gggcgccggc gcgatcgacg ccctggcctg cgatcagcac cggacgctcg gccgcgacga
     1321 ggagagcagc ggcctcgtcg agctggcgat ccgacggcgg cagcgcgccg atgcgatacg
     1381 cggcgggtgg cagcacgggc gccgtctgcg ggtcgccggt ctcatacaag atcggcgccg
     1441 gcagctcgac gtgcaccggg ccggggcggc cgttgtgcat ctcgcggaac gccagcctca
     1501 gcacctccgg aatccgagac cactcgaaga tggggccgcc ccacttgacc gccggccgga
     1561 aaaggtcgag ctggtcttgg ccttggaacg tcgagggcgg cgacgggtag acgatcccga
     1621 ggcggtgctg tgaggtgatc accaacaccg ggacgccctc atgcagcgcg gtgacgactc
     1681 ctggcagcag gttcgcggag cccgggccgg gattaccgag cacggccgcg actttgcccg
     1741 tcgtcttgta gaggccttcg gccatgtgta ccccggcagc ctcgtggcgc accggcacga
     1801 gccgaatgcc gtgcgcatcg agttgggcca acagcggatc gatctccggg gacggaaggc
     1861 cgaagagaaa ctcgatccct tccgcctgga gcgcacgtgc gaacagctcg ccaccggtga
     1921 tttcagtcat cttccacctc cacgtgcaga ctcatggata ggaccacccg cgtaggcata
     1981 agcgtagagg cgcaagtccc ggaccgacgg gaccttggtt ttgttcggag cggcaatgag
     2041 atcgttaggg cagcgagggg cgaacggacc ccgacctcaa ggagattcac gcatggcaca
     2101 gttcgaaacg agacagatag cagtcaacgg tgtgcgttca cccgtcctgg tcggcggtgc
     2161 cggcacatcc ggcgaggccg tcgtgttcgt tcatggcaac cctgatacgg gcagtgactg
     2221 gttaccgctg atggagccgg tcgccgagtt cgccaccgcg gtcgcccccg acatgccagg
     2281 atttggcggc gcggacaaac ctgagcactg ggattacacc gtggccgggt acgcgtcgca
     2341 tttgggtggc ttgctcgatc agctcggcat cgagcgggtg cacgtggtcg cccacgactt
     2401 cggcggccca ttcgccttga agtgggcggc ggacaacggc gagaagctcg ccagcgtcac
     2461 attgatcaac accggtgtgc tgctcggcta ccgctggcac aggctcgcgc gcatctggcg
     2521 gacaccggtt ctcggtgaag tgttcatgtt gacgacttct cctcggctcg cgcggttcgt
     2581 cgtgcaccac gacaatcccg ggttgcccga ggagtgggtg cagaagatcg cggaccatgc
     2641 caggccgccc gaaaccaagc gggcggtgct gcggctgtac cgttcgacgc cgccatccga
     2701 catggagctc gtcgccgggc cgctgcgcga gcgcgacccc gacaccctgg tcgtgtgggg
     2761 cgacgccgat gtttacatcc cggccgtgca agcccagcgg caacgagaag tgttctcgcg
     2821 cgcccgaatc gagatcctgc ccggagtcgg tcactgggca tggctcgagc aacccgaacg
     2881 tgtcgccgat cttgtggttc cgttcctgcg tgaacgggtt ggtggcacag cccaatagcc
     2941 ctcttggaca aggagatacg tgtgagcctc ttggaaccga cgccggagca gttcgccgca
     3001 ctggcggcgc ggcccgccga cgccgcagtg gtgatggtca acctgttgaa gttcaaccag
     3061 ccgggcggtc tcgagcgcta tctgcagtac gggcaggaag tcgctccgca cgtagagcgg
     3121 gtcggcggaa aagtccgtta cggcggagcc gcgccgactg tcgtcatcgg cgatggcgag
     3181 cggccgtggt gggacgcgat cctcgtcgtc gaatatccgt ctcccgccgc gttcatcgag
     3241 atggtgaccg acgacggtta tctgcaggtc caccaacatc gggtcgccgc actggaacgc
     3301 ggcgacctga tcgccacatc gacttggatg cctgccgact gagcatcttc cgcaccgctt
     3361 cgccggcccg cagcgactaa cttaagggca tgtatctgat cacgggggca gccggaaagg
     3421 tcggcggagt cagtcgctcg gtcgtcgaga tcctgatcgg caaaggggag tcggtgcgcg
     3481 cgatggtgca ccgcgaagac agtcgggcgc agcggctccg ggagctgggc gccgaagtgg
     3541 tgatcggaga cctgaccaac ccgcgcgatg tcgtccacgc gatgaccggt gtcgagcgga
     3601 tgttcttcaa catgagcgtg tcgctggatt acctggaagc cacggcgatc gtgtgcgcgg
     3661 cggcgcgcga ggtgggcggc tatgaggtga tcgtcaacat gtcgcagatg acggtctccg
     3721 agatgacgct gaccagcacg ggcgaatcct ttcagcagtg gctgcactgg ctgtccgagc
     3781 aggtcatgaa ctggtcgggc gtgccggtgg tgcacatccg gccgaccata tttctcgaca
     3841 acccgctgtt cacgatgctt gcgcgcaggt cggtgcatga tcgcggtgta cttccgctgc
     3901 ccttcggcac cggccgcacg tcgccgatcg cggccgccga cgtcgcccga ttgatcgccg
     3961 cggtcctgct cgatccggcc gaccggatcg gtgacgtcta tgagctgacg gggccggagg
     4021 tcctcggaat caacggcttg gccgagcact actcccgggc gctgaacagg tcggtggccg
     4081 cagaagacat cgcatacgac gactggatcc ggcaatacct gcgggcgtcc ggtctgcccg
     4141 accatgtcca gcagcacatc gccacaatgg cgcgccttca ccatgaggac cactacaacc
     4201 gcgtgagcga cgacttccag agggtgatcg gtgagcccgc gcagacggtc gagcagtaca
     4261 tcgcggcaca tccgcagcta ttcggcggtt aacggcgtat tgctctgaca tcttgggcct
     4321 tcggccccta ggcggtgcac cgcccaccga gcaggattgc cgccatggac ggaggaacag
     4381 gcccttcatc aagaacatga gctgatcttg gtggccggtt atcgagatct ggagatcgca
     4441 cggcgcgatt tcgccaagct ggccgacgag gtggaacgcg agcgatttct ggtcaagagg
     4501 gcagcgcttc tcaccaaaga cgagaatggc aacccaactg tcgtcgaact cggtaaccac
     4561 ctcggggcca agggcgccgg tttcggtgca ggcatcggga tcctggtggg actcttcgca
     4621 ccgccgattc tcggctcggt cctcatcggt gcggccgcgg gcgcgctcgt ggcggccttc
     4681 gcaggccacg agatgaaggt cggtctacga catgaggtca gccgagcgtt ggacgccgga
     4741 acggccgcgg tggttgcgct tgtgccgccg agcagtctga cgttcgccga gcgcgatttg
     4801 cttggggcgg acagcaagtc gatcgtccgg ttggacaagt cgaccgtcaa cagcctggag
     4861 aaagagatcg agcgtgccct gcacgagcac caacccgcgc agccggggtc ggacgctagc
     4921 actggcagcg tgggcaccag tagctgacgc gttccccgct ccaatcggtc tcgatcagag
     4981 tgccgcagcg gcggcacggt tgtccggggc ggccgtacac ccacagctcg ttgccgtggc
     5041 ggcgatctcc ggtggtggtg cggttccatc gcgctcgatt ggccaccatc atgtcgtggg
     5101 cccgtgacac cacccgcagc ggatcggcga cccgggcgac cggcgccgtg ggtaaaaccc
     5161 cgaatacgaa gcacaattcg ttgcagtaga cgttgccgac gccggccatg acgcgctggt
     5221 ccagcagcgc agccgcaatc ggccggtccg ggtcggcggt caagttcgcg gccgcccgcg
     5281 cgggatccca gtcggcgccg agcaggtctg ggccaagatg ggcgaccacc gacatgtcgt
     5341 cgtcgcgggc gagtatttcg agttgaccga gatcgatgcc cgctgcctga atgtc
//
LOCUS       DASWBF010000162        13656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_997351,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000162 DASWBF010000000
VERSION     DASWBF010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13656
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997351"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VF874_05415"
     CDS             complement(<1..113)
                     /locus_tag="VF874_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175544.1"
                     /translation="MADSSYRRLRRRRPLLRSIVELINALNGLQPLGRSGYR"
     gene            complement(117..935)
                     /locus_tag="VF874_05420"
     CDS             complement(117..935)
                     /locus_tag="VF874_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9175545.1"
                     /translation="MGDTDVDRVEIEKWDPEFTERVVGVLRPMLRRYFRTEVRGLDNI
                     PPAGGALLVSNHSGGQFAADVPVLAVEFYDKFGYDRPIHTLSHDMLFIGPVANVLLRT
                     GFIRANPGNAAAALRSGHLAVVFPGGDYDAMRPTVWENVIDFDGRKGYVRTAIEAGVP
                     IVPAVSIGGQENQLYLTRGTGLAKRLGPVARAARAKIMPLSFGFPFGFNVAGILPPNL
                     PLPTKIVTQVLEPIDIAAEFGDDPDVAEVDAHVREVMQRALDRLGRVRRLPILG"
     gene            979..1752
                     /locus_tag="VF874_05425"
     CDS             979..1752
                     /locus_tag="VF874_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9175546.1"
                     /translation="MSEDEAPAVLVEQRDRILLITINRPEAKNAVNAAVSRGLADAMD
                     RLDDDPGLSVGVLTGAGGSFCAGMDLKAFARGENVVVDGRGMGFNEKPPVKPLIAAVE
                     GYALAGGTELALATDLISAANNSAFGIPEVKRGLVAGGGGLLRLPQRIPYAIAMELAL
                     TGDNLSAERAHALGLVNVLTEPGKALDGALALAEKITANGPLAVAATKRIIVESRGWS
                     PDEMWREQVKIMMPVFASKDAKEGAIAFAEKRPPRWTGT"
     gene            1983..2375
                     /locus_tag="VF874_05430"
     CDS             1983..2375
                     /locus_tag="VF874_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175547.1"
                     /translation="MLTRAVLDNSGPHTCYVRADARRLPFNDETFDAVCCFGALYLMP
                     EPLRVASEMVRVLAPAGRIAILTTCTSSRPPDRYATVPVAHIVGLRMFDKETFVELFT
                     SAGLVDVQQEIQRALQYVTATKPANSAH"
     gene            2431..3795
                     /locus_tag="VF874_05435"
     CDS             2431..3795
                     /locus_tag="VF874_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9175548.1"
                     /translation="MADVDYCVIGAGFAGLTAALRLKQAGRSVALLEARDRIGGRTFT
                     EMLDDGTWIDRGGAWVGPGQDRIYAMMNEFGVGSYKQYLDGEAMMVVGGKQYRYSGTI
                     PLTMSPWAVANLGAVFLELGQMCKSIPLEEPWKAKNAAKWDQISLAKWLSGNTLSKPA
                     HDLLDTAISGCYTSAASEVSMLWVLYQMASGGGPNFVLSIKDGAQDARVVGGMGAISG
                     PMAAEIGDSVHLSQPVRRIEQDADGVTVRADDMTVRARRVVVAVPLVIANQILYEPML
                     PVDRTFLQQRVPSGAVFKINIVYDEPFWRADGLSGQSAAPNTPAPVTIEACTDKERPG
                     VMCVISEGPIAREACKLDESARRTAVLGELVRRFGDKAGSPEHYIEQNWTVERYSGGG
                     MIGHTGTGVLTEFGHALREPCGRIHWAGTESSAVMCGWIDGAIRSGERAASEVMAAES
                     VSMA"
     gene            3946..4332
                     /locus_tag="VF874_05440"
     CDS             3946..4332
                     /locus_tag="VF874_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9175549.1"
                     /translation="MSEQNIETVKRGYEAFSAGDVETVMSLFDDNIEWVQPGESAISG
                     IYRGKAEFGEYLRQLAEKALAVRALRFMAEGDTVVAFTEVTVGSEMGEDADVYKVRDG
                     KIVHAQIYTDTAMMERVFGRKEVAAG"
     gene            4728..5090
                     /locus_tag="VF874_05445"
     CDS             4728..5090
                     /locus_tag="VF874_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9175550.1"
                     /translation="MTAQIYTWLTVITISGEIDATNAGQLGHHVTALVPDDGALIVDM
                     VDTDFISVDGLRALFALNVECVRVDTRWAVIGSHAVHRLLRVADGEGLVPAVRSATEA
                     LQRVRRSRRERRPLQLVT"
     gene            5169..7003
                     /locus_tag="VF874_05450"
                     /pseudo
     CDS             5169..7003
                     /locus_tag="VF874_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            7053..7511
                     /locus_tag="VF874_05455"
     CDS             7053..7511
                     /locus_tag="VF874_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9175551.1"
                     /translation="MKVISSIDDAVGLVGQELGVGEWKKIDQKRIDAFADVTEDHQWI
                     HIDVDRAKTESPYGTTIAHGFLTLSMIPKLSKDNFRVENAKMAINYGLNKVRFLAAVP
                     SGGRIRARSELVDAAKTGDNMVNLTVRHTIELDGSDKPAAVADTIVRVIF"
     gene            complement(7608..9074)
                     /locus_tag="VF874_05460"
     CDS             complement(7608..9074)
                     /locus_tag="VF874_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX9175552.1"
                     /translation="MTEQYKAEWDKLFIGGEWTEPSTDKVIEVRSPATGEYVGKVPMA
                     AAADVDAAVAAARVAFDSGPWPSTPPAERAAVIQAAVKLLEDRKDYFTYLLTCETGQP
                     QTIIEMMHWTGSLGALSFFAGAADAIKWTETRNGAYGQSIVSREPIGVVGAVIAWNVP
                     LFLAVNKLGPALLAGCTIVLKPAAETPLSANALAEVFAEAGLPDGVLSVVPGDIETGQ
                     ALTANPDLDMLTFTGSSAVGKEVGKRAADMLKPSTLELGGKSAAILLEDVDLAAAIPM
                     MVFSGVMNAGQGCVNQTRILAPRSRYDEIVEAVKNFVQALPVGLPSDPAVQIGPLISE
                     KQRQRVEGYIAKGKEEGARLVCGDGRPEGLDDGYFVEPTVFADVDNKMTIAQEEIFGP
                     VLSIIPYDTEEDAIKIANDSVYGLAGSVWTTDVPKGIEISQKIRTGTYGINWYAFDPG
                     SPFGGYKNSGIGRECGPEGVEHFCQLKSVLLPMGYTVE"
     gene            complement(9088..10086)
                     /locus_tag="VF874_05465"
     CDS             complement(9088..10086)
                     /locus_tag="VF874_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9175553.1"
                     /translation="MAAQSTVSSSLRDVFEQWLAARQAGAAVATAALTADRTVFVDAG
                     WDGESHRLVVHLASSGPFETRSQFLTLRRLGTQLARPAVPPALWCEDDPAPLGAPFFV
                     MTRVDGLTTARYETPYTFASWITEASAAERERMQQATLEQLARVHAATPSDFAFLDRR
                     RPGESALSVIVRRLLEHYETVGSHGLRAPLIERAFGWLRDRWPVETTPVLCWGNARID
                     NVTYRDFAPVALASWDRATIGPREFDLGALVFHHRFVDGLAHAAGRPGLPDFLQPGDV
                     AATYADITGYRPADLDFYIVFAALQHAIETIRSPLRTKAFQALDDAMADERSREEQ"
     gene            complement(10147..10908)
                     /locus_tag="VF874_05470"
     CDS             complement(10147..10908)
                     /locus_tag="VF874_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175554.1"
                     /translation="MPPTAVFARRATLRRSVRLLSEFRYEQRQPDRFYGALAADTAAL
                     VDDLWRGVHGESAAGRSLLDVGGGPGYFASAFTDRGLRYLGVEPDPAEMHAAPCARSG
                     TGTFVRASGMALPFADDSVDIALSSNVAEHVAAPWRLGDELLRVTRPGGLVVLSYTVW
                     LGPFGGHEMGLTHYLGGARAARLYARKHGHPAKNNYGSSLFAVSVADGLCWARSTGAL
                     VAAFPRYHPRWAWWMTSVPLLREFAVSNLVLVLAP"
     gene            10933..12102
                     /locus_tag="VF874_05475"
     CDS             10933..12102
                     /locus_tag="VF874_05475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9175555.1"
                     /translation="MSSAEAQPGRVGSVLMLCWRDVGHPQGGGSETYLQRIGAQLAAD
                     GMSVTLRAARYDGAVRDEVVDGVRISRAGGHYSVYLWALAAMIGARLGVGPLRGVRPD
                     VIVDTQNGWPFFARLVFGRRVAVLVHHCHREQWPLAGRRLGRLGWFCESTISPRVHRR
                     NQYVTVSLPSARDLTDLGVSVNRIAVVRNGLDKTPALRMPRSATPRVVVLSRLVPHKH
                     IEDALDAIAVLRTRIPGLHLDIVGDGWWRDRLVSHAARLGIADSVTFHGHVADAAKHF
                     VLQQAWVHVLPSCKEGWGLAVTEAAQHGVPTIGYRSSGGLTDSIIDGVTGVLVDDRDQ
                     LVYRLEELLTDPLLRDELGRKAQAHSAEFSWRQSADAMRVVLEAVAAGRLVSGVV"
     gene            complement(12129..>13656)
                     /locus_tag="VF874_05480"
     CDS             complement(12129..>13656)
                     /locus_tag="VF874_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175556.1"
                     /translation="LASRLIDGGIVVKALLILGLWLAGWGAARLVALVISDAGLGGQL
                     VAVTVAIWNPYVAERLLQGHWSLLVGYGCLPWVAEAMLKLRDGSGSWWALVWWTAVAG
                     LTPTGLMLAATVALVCAVVPGGGRPRWLCAAAGLGVAVLAALPWLTASVFSGLSSGDS
                     GIAAFAARAEPGLGTLGSLAAMGGIWNAEAVPGSRGTLFAVVGSAVVLGIVAAGLPAV
                     RRRPAAVALLVLGAVAVVVPAAMATGLGVAVVDAVVRAAPGLGVVRDGQKWVALAMPG
                     YAVAGAGAVVTLRRWVPAGAVAAVCCAALIAVLPDLAWGVGGAMTPVRYPTGWAAVAS
                     RINADPRPVAVLPAGTMRRFGWSGPAPVLDPLPRWVRAHVWSTGDLTISGRTVLGEGS
                     RARSVQQLLLDGAGPAALARAGVGWVIVESGSPGEIGWAAKTLASLPVVYHDEDITLY
                     RVGGDDPGASRTHRTVMIGAHLAWLSVLAAGAAGTTFASVRRHIRRNAESHVLHRKFE
                     "
BASE COUNT         2343 a         4581 c         4583 g         2149 t
ORIGIN      
        1 cggtagccgc tacggccgag cggctgtaac ccgttgagcg cgttgatcaa ttcgacaatc
       61 gaacgaagca gcgggcgccg ccgccgcaag cgccgatacg aggagtcggc catcgctcaa
      121 ccgaggatcg ggagccggcg cacacgcccc agccggtcga gagcccgctg catcacctcg
      181 cgcacgtggg cgtcgacctc ggcgacgtcg ggatcgtcgc cgaattccgc cgcgatgtcg
      241 atgggctcaa gcacttgcgt gacgatcttg gtgggcagtg gcaggttggg cggcaggatc
      301 ccggcgacgt tgaacccgaa cgggaatccg aacgacagtg gcatgatctt cgcgcgtgcc
      361 gcccgtgcga cggggccgag ccgcttcgcc aacccggttc cacgggtcaa gtacagctgg
      421 ttctcctgac cgccgatcga caccgcaggc acaatcggca cgccggcttc gattgctgtc
      481 ctgacatatc ccttccggcc gtcgaagtcg atcacgttct cccagaccgt tggccgcatc
      541 gcgtcgtagt cgccgcccgg gaacaccacc gcgaggtggc cggagcgcag cgccgccgcg
      601 gcgttccccg ggttcgcccg gatgaagccg gtgcgcagca acacgtttgc caccggcccg
      661 atgaacagca tgtcgtggct cagcgtatgg atcgggcggt catagccgaa cttgtcgtag
      721 aactcgacgg caaggaccgg aacgtcggcc gcgaactgcc cgccggagtg gttcgagacg
      781 agcagcgctc cgcccgcggg cgggatgttg tccaggccgc gcacctcggt ccggaaatac
      841 cgtcgaagca tcggccgcag gacaccgacg acacgctcgg tgaactcggg atcccacttc
      901 tcgatctcga cacgatcgac gtcggtgtca cccatggtcg aatggtaccg acgggccgtc
      961 cggtactttc aggtactcat gagcgaagac gaagcccccg ccgtccttgt cgagcagcgc
     1021 gaccggatcc tgctgatcac catcaaccgc cccgaggcca agaacgcggt caacgccgcg
     1081 gtcagccgag gccttgccga tgccatggac cggctggacg acgacccggg tctgtcggtc
     1141 ggggtgttga ccggtgccgg cggctcgttc tgtgccggca tggacctcaa ggccttcgcg
     1201 cgcggcgaga acgtcgtcgt cgacggccgc ggcatgggct tcaacgagaa gccgccggtc
     1261 aagccgctga tcgcggcggt cgaggggtac gcgctggccg gcggcactga gctggcgctg
     1321 gccaccgacc tgatctcggc ggccaacaac tcggcgttcg gcattccgga ggtcaagcgc
     1381 gggctggtcg ccggcggcgg tgggttgctg cggctgccgc agcggatccc gtacgcgatc
     1441 gcgatggagc tggcgctgac gggcgacaac ctgtccgccg agcgcgcgca tgcgctgggt
     1501 ctggtcaacg tgctcaccga gccgggcaag gcgctggacg gcgcgctggc gctggccgag
     1561 aagatcacgg ccaacgggcc gctcgcggtc gccgccacca agcggatcat cgtcgagtcc
     1621 cgcggctgga gccccgacga gatgtggcgc gagcaggtca agatcatgat gccggtgttc
     1681 gcgtccaagg acgccaagga gggcgcgatt gcgttcgccg agaagcgccc gccgcgctgg
     1741 acgggaacct gattagcagg tgacgccgat cgaggtgacc cgcggcctgc tcaaccagga
     1801 cgccgcgctg cggcagggct acctcgatgt gctgggcggg tcggtgagct ctccggttcc
     1861 cacggccgcc cagcgcgcga tgaacagcca gttcgtcgcc ggggtgtatg agcgggtgtg
     1921 gcggccgctg tccttctacg tcgccagcgg cgtgaccacc ggcgcggagc aacgccgagc
     1981 cgatgctcac ccgtgccgtg ctcgacaaca gcggaccgca cacctgctac gtgcgcgccg
     2041 acgcgaggcg gttgccgttc aacgacgaaa cgttcgacgc ggtctgctgt ttcggcgcgc
     2101 tgtacctgat gcctgagccg ctgcgcgtcg cgtcggaaat ggtgcgggtg ctggcgcctg
     2161 cggggcggat cgcgattttg accacctgca cgagcagccg tccacccgac cggtacgcga
     2221 cggttcctgt ggcacatatc gtcggcttgc gaatgtttga caaggaaact ttcgtggagc
     2281 tcttcacgtc cgccgggttg gtcgacgtgc agcaggagat tcagcgcgcc ctgcagtacg
     2341 tcaccgccac gaaaccggcg aattccgccc actgactttt gccgtgcgcg atagttgcgg
     2401 ctatccggca cagctaaccg aggagccatc atggccgacg tcgactactg cgtaattggt
     2461 gcgggttttg cgggactgac tgccgcgctg cggctgaagc aggcgggccg ttcggtggcg
     2521 ttgctggagg cccgcgacag aatcggcggc cgcacgttca ccgaaatgct tgacgacggc
     2581 acgtggatcg accgcggcgg cgcctgggtg ggcccaggac aagaccgcat ctacgcgatg
     2641 atgaacgagt tcggggtcgg cagctacaag cagtacctcg acggcgaagc gatgatggtc
     2701 gtcggcggca agcagtaccg ctattccggc acgattccgt tgaccatgag cccgtgggcc
     2761 gtcgccaatc tcggtgcggt cttcctcgag ctggggcaga tgtgcaagtc gatcccactg
     2821 gaggagccct ggaaagcgaa gaacgccgcg aagtgggacc agatcagcct ggccaagtgg
     2881 ctcagtggca acacactgtc caagcctgcc cacgacctgc tggacacggc catctccggc
     2941 tgctacacct ccgccgcgtc tgaggtctcg atgctgtggg tgctctacca aatggcgtcg
     3001 ggcggcggtc cgaactttgt cctcagcatc aaggacggag cgcaagatgc ccgggtggtt
     3061 ggcggaatgg gcgcgatctc cggaccgatg gccgctgaga tcggcgactc cgtgcacctc
     3121 tctcaacccg tccgtcggat cgagcaggat gccgacgggg tgacggtgcg cgccgacgac
     3181 atgacggtgc gcgcacgccg cgtcgtcgtc gcggtcccgc tcgtcattgc gaaccagatc
     3241 ctctacgaac cgatgctgcc cgtggaccgg acatttctgc agcagcgggt gcccagcggc
     3301 gccgtcttca agatcaacat cgtgtacgac gagccgttct ggcgcgccga cgggttgtcg
     3361 gggcagtcgg cggcacccaa cacgcccgcg ccggtgacca tcgaggcatg caccgacaag
     3421 gaacgacccg gagtgatgtg tgtcatcagc gagggtccga tcgctcgtga agcctgcaaa
     3481 ctcgacgagt ccgcgcggcg cacggcggtc ctcggcgagc tcgtcaggcg gttcggcgac
     3541 aaggcgggat cgcccgagca ctacatcgaa cagaattgga ctgtcgagcg gtactccggc
     3601 ggcggaatga tcggccacac ggggaccggc gtgctcacgg aattcgggca cgcactgcgc
     3661 gagccgtgcg gccgcattca ctgggccggc accgaaagct cggcggtgat gtgcggctgg
     3721 atcgacggcg cgatccgctc cggtgagcgt gccgccagcg aagtgatggc cgccgagtcg
     3781 gtctctatgg cttgacgccc tcgccctacg cctcgagact gcggacagat cgcacttttc
     3841 gctcgaaagg cgatctctcc gcagtctgga tggcggacaa tagttgtggc gtgtcggttc
     3901 aggccgtgcg ctgccgaatg tggttacgca ggaaagggat tcgctatgtc agagcagaac
     3961 atcgaaacgg tcaagagagg atatgaagcg ttctccgccg gcgacgtcga aacggtgatg
     4021 agcctgttcg acgacaacat cgaatgggta cagccgggtg aaagcgcgat cagcggtatc
     4081 tatcgcggca aggctgaatt cggcgagtac ctcaggcagt tggccgagaa agccctggcg
     4141 gtcagggccc tgcgattcat ggccgagggc gacacggtcg tcgcgttcac tgaggtaact
     4201 gtcggcagcg aaatgggcga agacgccgac gtgtacaagg tgcgcgacgg caagatcgtg
     4261 cacgcgcaga tctacacgga cacggcgatg atggagcgcg tgttcggccg caaggaggtc
     4321 gccgccgggt agcccctgcg tatggtcggc aggtgatgtc gcgacgcggc tgttcgtgtc
     4381 accccgcacg gtgcagttgg atgatgcaca ggtggcatac ctttcccact gctgggacgt
     4441 taccgttaag gaaccgttag aactcggcgg cgtttgcttg ccagctgcat gtctgggcgc
     4501 gtgatccgcg ctacactctc cgcaaggttg agatcacagc ctgtgatgaa aacgtcccgc
     4561 accgcaaact gacaggctgt tcaattcatg ccaatgcgcg ccgggcgttt tcgaccgaga
     4621 tcagcgacag atccatgagg gataagtcaa tgactgctgt gcattccgca tccgccgccc
     4681 ctgacgcact acatggcaca tcggtaatcg attgtggccg tgcgtttttg acggcgcaga
     4741 tctacacctg gctgacggtt atcacgatca gcggcgagat agacgcgaca aacgcaggcc
     4801 agctcggcca ccacgtcacc gcgctcgtgc ccgacgacgg tgctctcatc gtcgatatgg
     4861 tcgacacgga cttcatcagt gtcgacggcc tgcgtgcgct gttcgcgctg aacgtcgagt
     4921 gcgtgcgtgt cgacacgcgg tgggcggtga tcggcagcca cgccgtgcac cggctgctgc
     4981 gggtcgccga cggcgagggg cttgttcccg cggtgcgatc ggcgaccgag gcattgcagc
     5041 gcgtgcgccg ctcgcggcgt gagcgacgcc cgctgcaact ggtgacctga caacggtcgc
     5101 cgttaccggg cccggcctgc gccggaggac tacgctcagc gccatcgcga cgagggaagg
     5161 cgccgggcat gggtcactac aagagcaacg tccgcgacct cgagttcaac ctgttcgagg
     5221 ttctagaact cgacaaggcg ctcgcgagcg gggagttcgg cgacctcgac ggcgagtcgg
     5281 tgcggcacat gctcgccgag gcgtcccggc tggctgaggg tcccgtcgcg gagtcgttcg
     5341 ccgatgcgga ccgccatccg ccgacgttcg acaccagcga gcacacggtg acgctgccgg
     5401 agccgttcaa gaagtcactg cgcgcgtggc agcagggtga gtggtttcgc gtcgggctcg
     5461 accagaacgt cggcggggtg cccgcgccgg cgatggtcca atgggccatc aacgagttcg
     5521 tgctcggcgc gaacccggcg gtgttcctgt atctcgccgg tccgatattc gccaacatcc
     5581 tgtaccacat cggcaacgag cagcagcggc attgggccga gttggcgatc gagcgcaact
     5641 gggccgccac gatggtgctg acggagcccg acgcgggttc ggacgtcggc gcgggccgga
     5701 ccaaggcgat cccacaaccg gacggcacct ggcacatcga gggtgtcaag cggttcatca
     5761 ccaacggcga caccgacgac ctcttcgaga acatccttca cgtcgtgctg gcccgccccg
     5821 agggcgcagc tccgggcacc aaggggctca gcctgtttct ggtgccgaag tacctgctcg
     5881 accccgaaac cggcgcaccg ggggaacgca acggcgtctt cgtcaccggc ctggaacgca
     5941 agatgggtct aaaggtctcg gcgacaacgg aattgacctt cggcgggcac ggtaccccgg
     6001 ccatcggctg gctcgtcggc gacgtccacc ggggcattgc gcagatgttc gagctgatcg
     6061 agtacgcacg aatgatggtg ggtactaagg ccatttcgac gctgtcgacc ggttatctga
     6121 acgcgctgga ttacgccaag acccgcgttc agggcgccga catgacacag atgaccgaca
     6181 agaccgcgcc gcgcgtgacg atcatccatc accccgacgt gcgacgggcg ttgatgacgc
     6241 agaaggccta cgtcgaaggc ctgcgcgcgc tgtatctgta caccgcggcc caccatgatc
     6301 cgagcgtggc gggggtggtg tcgggcgccg acgccgacct cgcggggcgg gtcaacgatc
     6361 tgctgttgcc gatcgtcaag ggcgtcggct ccgaacgcgc ctaccagtgc ctgaccgagt
     6421 ccctgcagac gctgggcggt tcgggattcc tgcaggacta tccgatcgag cagtacatcc
     6481 gcgacagcaa gatcgacagc ctgtatgagg gcaccaccgc aatacaggcc caggacttct
     6541 tcttccgcaa gatcgctcga gaccagggcg tcgcgctgca gcacgtggtg ggccagattc
     6601 agaagtgcct cgatggcgac ggggcacgcg aggagctcgc ggaaagccgg gcgctgctgg
     6661 cgaccgcgat gtgcgacctt caggcgatgg tcgccgcgct cacggggtat ctgatcgagt
     6721 cccagaacaa cgcgcgcgaa ctgtaccgcg tcggcctcga gtcggtgccg ttcttgctgt
     6781 cggtgggtga cctgctgatt ggctggctgc tggtacggca ggccgagatc gcgctgcaag
     6841 cactcgacgg cgacgttgcc gacgacgacc gtgcgttcta cctcggaaag gtttcggcgg
     6901 caaagttttt caccaagaat gtgttacccc ggctgtcgcc gaacgccgca tcgtcgaggc
     6961 cgtcgacctc acgatcatgg agatgcgcga ggagtccttc tagtagggcg cgttaaggtc
     7021 tgattcgcag cagcagcgga agggagcacg caatgaaggt catttcatcc attgacgacg
     7081 ccgtcggtct tgtcggccag gaactcgggg tcggcgagtg gaagaagatc gaccagaagc
     7141 gcatcgacgc attcgccgac gtcaccgagg atcaccagtg gattcacatc gacgtcgatc
     7201 gcgccaagac cgaaagccct tacggcacaa cgattgctca cggattcctg acgctgtcga
     7261 tgatcccgaa gctgtccaag gacaacttcc gcgtcgagaa cgccaagatg gcgatcaact
     7321 acgggctcaa caaggtccgg ttcctggccg cggtcccgtc cggcgggcgg atccgcgcac
     7381 ggtcggagct cgtcgacgcc gcgaagaccg gcgacaacat ggtcaacctc accgtgcggc
     7441 acaccatcga gctggatggg tcggacaagc cggccgccgt cgccgacacg atcgtgcggg
     7501 tgatcttcta acccgttcta accccgccgc gaaacccccg tttcgcacag aaagtgcgag
     7561 gcgaacgtgc gaaactccgg tttcgcgcga cgaggcggcg gcgtgggcta ctcgacggtg
     7621 tagcccatcg gcagcagcac gctcttgagc tggcagaagt gctccacacc ttccgggccg
     7681 cactcacggc cgatgcctga gttcttgtag ccgccgaacg gtgagccggg atcgaacgcg
     7741 taccagttga ttccgtacgt gccggtgcgg atcttctgcg aaatctcgat gcccttgggc
     7801 acatcggtgg tccagacgct gccggccaga ccgtagaccg agtcgttggc gatcttgatc
     7861 gcgtcctcct cggtgtcata aggaatgatc gacagcaccg gcccgaagat ctcctcctgc
     7921 gcgatcgtca tcttgttgtc gacgtcggcg aacacggtcg gctccacgaa gtagccgtcg
     7981 tccagcccct cgggccggcc atccccgcac actagccgcg cgccttcctc cttgcctttg
     8041 gcgatgtagc cttcgacgcg ctgccgctgc ttctcactga tcagcggacc gatctgaacc
     8101 gccggatccg acggcagccc gaccggaagc gcctgcacga aattctttac tgcctccacg
     8161 atttcgtcgt agcgcgaccg cggcgcgagg atccgggtct ggttcacgca gccctggccg
     8221 gcgttcatca cgccggagaa caccatcatc ggtatggccg cagcgaggtc gacgtcttcc
     8281 aacaagatcg ccgccgactt gccgcccagc tcaagggtgc tcggcttcag catgtcggcg
     8341 gcgcgcttgc cgacctcctt gcccaccgcc gagctgcccg tgaacgtcag catgtcgagg
     8401 tcggggttgg cggtcagcgc ctgcccggtc tcgatgtcac cgggcaccac tgacagcacc
     8461 ccgtcgggca ggccggcctc ggcgaacacc tcggcaagtg cgttggcgct caacggggtc
     8521 tccgcggcgg gcttgagcac gatcgtgcag ccggccagca gcgccgggcc gagcttgttc
     8581 acggccagga acagcggcac gttccacgcg atgaccgcac cgaccacgcc gatcggttca
     8641 cgcgagacga tcgactgccc gtacgcaccg ttgcgggtct cggtccattt gatggcgtcg
     8701 gccgcaccgg cgaaaaagct cagtgcgcca agcgatccgg tccagtgcat catttcgatg
     8761 atcgtctggg gctgcccggt ctcacaggtc agcagatagg tgaaatagtc cttgcggtct
     8821 tcgaggagct tgacggcggc ctgaatcacc gcagcgcgtt cggccggcgg cgtggacggc
     8881 catgggccgc tgtcgaacgc gacgcgggcc gccgccacgg cggcgtcgac gtcggcggca
     8941 gccgccatcg gcactttgcc gacgtactcg ccggtggcag gggagcgcac ctcgatgacc
     9001 ttgtcggtcg acggctcggt ccactccccg ccgatgaaca acttgtccca ttcggccttg
     9061 tactgctcgg tcatctctct ccttccgtta ttgctcttcg cgcgagcgct catcggccat
     9121 cgcgtcgtcg agcgcctgga aagctttggt gcgcaacggg cttcgtatcg tttcgatggc
     9181 gtgctgcaat gcggcgaaga cgatgtagaa gtccagatcc gctggccggt agccggtgat
     9241 gtcggcgtat gtggcggcga cgtcgccggg ctgcaggaag tcgggcagac cgggccgccc
     9301 ggcggcatgg gcgaggccgt cgacgaagcg gtggtgaaag accaacgccc ccaagtcgaa
     9361 ttcccgcggt ccaatggtcg cgcggtccca actcgccagc gccacgggcg cgaagtcccg
     9421 gtatgtgacg ttgtcgattc gcgcatttcc ccagcagagc accggcgtcg tctcgacggg
     9481 ccagcggtcc cgcagccacc cgaacgcgcg ctcgatcagc ggtgcccgaa gtccatggga
     9541 gccgacggtc tcgtaatgtt cgagcaggcg ccgaacgatg accgacagcg ccgactcacc
     9601 cggacggcga cgatcgagga aggcgaaatc ggacggcgtc gccgcgtgca ctcgggccag
     9661 ttgttccaac gtcgcctgct gcatgcgttc gcgttcggcg gcgctcgcct cggtgatcca
     9721 cgaggcgaac gtgtacggcg tttcgtacct cgcggtcgtc agtccgtcga cacgggtcat
     9781 cacgaagaag ggagcgccga ggggcgcggg gtcgtcttcg caccacagcg ccggcggcac
     9841 cgccggccga gcgagctgcg tgcccagccg tcgcagcgtc aggaactgag atcgcgtctc
     9901 gaacgggccg ctggaggcca ggtgcaccac cagccggtgc gattccccat cccagccggc
     9961 gtcgacgaac accgtgcggt cggcggtcag cgccgccgtc gccaccgccg ctccggcctg
    10021 tcgcgcggcc agccactgct cgaaaacgtc gcgtagggag ctactgactg ttgactgcgc
    10081 tgccatggtg tgacccacac tacccagccg ccgacgaaac gagaacctgt tccagtctga
    10141 acgcggtcac ggcgcaagca ccagcaccag attgctcacc gcgaactccc gcaacagcgg
    10201 caccgacgtc atccaccagg cccatcgcgg gtggtagcgg ggaaatgcgg cgacgagcgc
    10261 gcccgtggag cgagcccagc acaggccgtc ggccaccgac acggcgaaca gcgacgagcc
    10321 gtagttgttc ttcgccggat ggccgtgctt gcgggcatag aggcgcgcgg cgcgggcgcc
    10381 gccgaggtag tgcgtcagac ccatttcgtg tccgccgaac gggccgagcc atacggtgta
    10441 ggacaacacg acgagcccgc cgggccgggt cacgcgcagc agttcgtcgc cgagccgcca
    10501 gggcgcggcc acgtgctcgg ccacgttgga tgacagggcg atgtcgacgc tgtcgtcggc
    10561 gaacggcagc gccatgccgg aagcgcggac gaaggtgcca gtcccgctgc gcgcgcaggg
    10621 cgccgcgtgc atttcggccg gatccggctc gacgcccaga tagcgcagcc cgcggtcggt
    10681 gaacgcagac gcgaagtagc cggggccgcc gcccacgtcg agcagcgacc ggccggccgc
    10741 cgactcgccg tgcacgccac gccagagatc gtcgacgagc gcggcggtgt cggccgccag
    10801 cgcgccgtag aaccggtccg gttgacgttg ctcgtagcgg aattccgaca gcagccgcac
    10861 cgaccggcgc agggttgccc gtctagcgaa gacggcggtg ggcggcattc gcgtgaggct
    10921 acgctggacc ggatgtcgtc cgccgaagcc caacccggac gcgtcggctc agtgctgatg
    10981 ctgtgctggc gcgacgtcgg ccacccgcag ggcggcggca gcgaaaccta cctgcagcgc
    11041 atcggggctc aactcgcagc cgacgggatg tcggtgacgc tgcgggccgc gcgctacgac
    11101 ggtgcggtcc gcgacgaggt ggtcgacggc gtgcggatca gccgcgccgg cggccactac
    11161 tcggtgtacc tgtgggcgct ggcggcgatg atcggtgcgc gtctgggagt cggcccgttg
    11221 cgcggcgtgc ggcccgacgt gatcgtcgac acccagaacg gctggccgtt tttcgctcgg
    11281 ctggtgtttg gacgtcgggt ggcggtgttg gtgcatcact gtcaccgcga gcagtggccg
    11341 ctggccggcc gccgactcgg ccggctcggc tggttctgtg agtcgacgat ttcgccgcgg
    11401 gtgcaccggc gcaaccagta cgtgaccgtg tcgctgccgt cggcccgaga tctcaccgac
    11461 ctcggggtga gtgtgaaccg aattgccgtg gtgcgcaacg gccttgacaa gacgcctgca
    11521 ctgcggatgc cgcgatcggc gactccgcgg gtggtggtgc tgtcgcggct ggtgccgcac
    11581 aagcacatcg aggacgcgct tgatgccatt gcggtgctgc ggacccgcat tcccggcctg
    11641 catctcgaca tcgtcggcga cggttggtgg cgggatcgac tggtgtccca cgcggcgcgg
    11701 ctcggtatcg ccgactcggt gacgttccac gggcacgtcg ccgatgcagc caaacacttt
    11761 gtgctgcaac aagcttgggt gcatgtactg ccgtcgtgca aagaggggtg gggcctggcg
    11821 gtgaccgagg ccgcccagca cggcgtgccg accatcggct atcgctcgtc cggcgggctc
    11881 accgactcga tcatcgacgg ggtcaccggc gtgctggtgg acgaccgcga tcagcttgtc
    11941 taccggctcg aggagctgct caccgatccg ctgctgcgcg acgagctggg ccgcaaggcg
    12001 caagcccaca gcgccgagtt ctcttggcgc caaagcgccg acgcgatgcg cgtggtgctc
    12061 gaggcggtgg cggcgggccg gctggtcagc ggcgtggtgt aggtgcgaga gcgacgtttt
    12121 gcagagggtt actcgaactt cctatgtaaa acgtggcttt cggcgttccg acggatgtgc
    12181 cttcgcaccg aggcgaatgt cgtccccgcc gcgccggccg caagcacgct cagccacgcc
    12241 aggtgcgcac cgatcatcac cgtgcgatgc gtccggctgg cacccggatc gtcaccgccg
    12301 actcgataca gcgtgatatc ctcgtcgtgg tagaccaccg gcagcgaagc caaagtcttt
    12361 gctgcccagc ctatttcgcc tggcgacccc gattcgacga tcacccatcc cacgccggcg
    12421 cgggccagcg cggcggggcc ggcaccgtcg agaagcagtt gttgcacact gcgcgcccgg
    12481 ctgccttcgc cgagcaccgt gcgccccgag atcgttaggt cgccggtcga ccacacatgc
    12541 gcacgcaccc aacgaggcag cggatcgagc accggtgccg ggccggacca gccgaaccgt
    12601 cgcatcgtgc cggccggcaa caccgcgacc ggccgcgggt cggcgttgat gcgcgacgcc
    12661 accgccgccc acccggtggg atagcggacc ggcgtcatcg cgccgccgac gccccaggca
    12721 agatccggca gcaccgcgat caaagcagcg cagcacaccg ccgcgaccgc gccagcaggg
    12781 acccaacgcc gcagcgtcac caccgcgccc gcgccggcca ccgcgtatcc cggcatcgcc
    12841 agcgcgaccc acttctgccc gtcgcggacc accccgagac caggcgctgc ccgcaccacc
    12901 gcgtcgacga cggcgacgcc gaggccggtg gccatcgcgg cgggcaccac caccgcgacc
    12961 gcgcccagca ccaacagcgc gaccgcggcc ggtcgtcgcc gcaccgcagg cagtcccgcg
    13021 gcgacgatgc ccagcaccac cgccgagccg acgacggcga acagcgtgcc gcgtgacccc
    13081 ggaaccgcct cggcgttcca gatgccgccc atcgccgcca gactgcccag tgttcccagc
    13141 ccgggttcgg cccgtgccgc gaatgccgcg atcccgctgt cccccgacga cagacccgag
    13201 aacacactgg cggtcagcca cggcaacgcc gccagcacgg ccacacccag acccgccgcc
    13261 gcgcagagcc atcgcggccg cccgccgccc ggcaccaccg cgcacaccag cgcgaccgtc
    13321 gccgccagca tcaggcccgt cggcgtcaac ccggccactg cggtccacca caccagcgcc
    13381 caccaactcc ccgatccgtc gcgcagcttc agcatcgctt ccgcgaccca cggcagacat
    13441 ccgtagccga ccagcagact ccagtggcct tgcaaaagac gttctgccac atagggattc
    13501 cagatggcga ccgtcaccgc gacgagttgc cccccgagtc cggcgtcgga aatcaccagc
    13561 gccacaagcc gcgccgcacc ccagccggcc agccacagcc cgaggatcaa cagcgccttg
    13621 accacgatcc cgccgtcgat cagccgggat gcgagt
//
LOCUS       DASWBF010000163         5152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_938727,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000163 DASWBF010000000
VERSION     DASWBF010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938727"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VF874_05485"
     CDS             <1..801
                     /locus_tag="VF874_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9175557.1"
                     /translation="RTLDGWQDWAKQRGHRGLAYVLVGDDGTLSGPVAKNLSDAERDG
                     LAAHVGAKPGDCVFFSAGPAKSSRALLGAARSEIARRLDMIDPSAWAFVWVVDPPLFE
                     PADEAAAHGDVAVGSGAWTAVHHAFTAPKPECVDAIDDDPGSVLADAYDIVCNGHEIG
                     GGSIRIHRRDIQERVFAVMGIDKAEAEEKFGFLLNAFTFGAPPHGGIAFGWDRITALL
                     AGMDSIREVIAFPKTGGGVDPLTDAPTPITPAQLKDAGIAVIARKDEK"
     gene            798..1229
                     /locus_tag="VF874_05490"
     CDS             798..1229
                     /locus_tag="VF874_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9175558.1"
                     /translation="MSDMPDPAELKGFNAKIIEEFRTNGGKVGGQFEGADLLLLTTTG
                     AKSGQPRVSPLAYQRVDGKMLIIGSYRGADVDPAWVHNLRANPQAHVEVGSDSYDVVT
                     RELPPEERDQAWDEIVAAAPGFGEYQSNTSRVIPLFELTRA"
     gene            complement(1341..2756)
                     /locus_tag="VF874_05495"
     CDS             complement(1341..2756)
                     /locus_tag="VF874_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HEX9175559.1"
                     /translation="MPTTDPTFYRSPSAAVAAPPEQLAYVAAFDPASQRKDAIAVVDC
                     DPNSATFGSVVGWTDLPTPGNELHHFGWNACSSALCHEGHAGHHGATVERRYLVVPGI
                     RSSNTYVLDTKPDPRQPVLSHTIKAAELAAKAGYSRPHTVHCGPGGIFMSALGGVNGD
                     DGPGGVALIDHDTFEVIGPWEVDRGPQFLAYDVWWHLKYDTVMTSEWATPAMIENGLN
                     PEDLLGRKFGHHLNFWSMSERKLTQRVDLGDQHQMVLELRPAHDPSQAWGFASVVISV
                     EDLSASVWLWHKDGDQWSVEKVITIPAEPADADDLPPLLKPFGAAAPLVTDIDLSVDD
                     RWLYVSCFGTGELKQYDVSDPFHPRETASVRLGGIVRREPHPAAPGLPLGGGPQMVEI
                     SRDGRRVYVTNSLYAAWDEVFYPDGVGAWMAKMDADVAAGGLVLDGRFFPHGDDFRGL
                     RVHQTRLQGGDASSDSYCFTN"
     gene            2868..3203
                     /locus_tag="VF874_05500"
     CDS             2868..3203
                     /locus_tag="VF874_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175560.1"
                     /translation="MTTDDGNMYGAPPGSGISMPPYYLPTPSVKNRNNYFPQSERIGD
                     GEIRITFMGSNPWAPRLSQAGTCTMVELGTAGRLTQVRCHLIEIPAHAEHLVSLEHTP
                     CAQEGGSFM"
     gene            3329..3592
                     /locus_tag="VF874_05505"
     CDS             3329..3592
                     /locus_tag="VF874_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175561.1"
                     /translation="MATIRLTTRRTVVALGIAAAIAAAPAVATFAGSPHVLADPGECT
                     TTGEPGDASLNCAPAFVPEIGAPSEMELTDTNPGIGSPEHSHH"
     gene            3616..4548
                     /locus_tag="VF874_05510"
     CDS             3616..4548
                     /locus_tag="VF874_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEX9175562.1"
                     /translation="MLHLRVIAPTELRESVIDVLRRDKGVTHVVVHRNAALDPPGDEV
                     TADIAREAANDVIDDLKELGLKRRGAITLDVVDTVVSTAAYRAEKEAEGDPADAVIWD
                     ELAARTREESSLNVTYLTFLSLACLIAAVGVVTDSPVTVVGAMVVGPEFGPLAALAVA
                     IVRRRGYLARRAALALLVGFPLAMFVTAVGVLVGEAVGWITLATTKQLEEVDFIFQVG
                     PLSLVVALFAGAAGMLSLVSAKSSALVGVFISVTTVPAAGFAVVAATVGDWDIAAKSA
                     TQLGVNLAGIVFAGVLVLWVYLRLTSHHRAVRRT"
     gene            4592..>5152
                     /locus_tag="VF874_05515"
     CDS             4592..>5152
                     /locus_tag="VF874_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9175563.1"
                     /translation="MGIKVALEHRTSYTFDRLVEVYPHVVRLRPAPHSRTPIEAYSLT
                     VEPADHFINWQQDAFGNFLARLVFPNRTRQLTITVGLIADMKVINPFDFFIEDWAETV
                     PFALRPSGPPQAGGAPTSTPFEYPKAMAEDLQPYLRPVDEDDDGSGPGELVRKWVSRF
                     EVPKGTRTIDFLVALNRAVNADVAYSV"
BASE COUNT          860 a         1710 c         1734 g          848 t
ORIGIN      
        1 cgcacgctgg acggctggca ggactgggcc aagcagcgtg ggcaccgcgg gctcgcgtac
       61 gtgctcgtcg gcgacgacgg cacgctgagc gggccggtgg ccaagaacct ttcggacgcc
      121 gaacgtgacg ggctggccgc ccatgtcggc gcgaagcccg gcgactgcgt cttcttctcg
      181 gccgggcctg cgaagtcgtc gcgcgcgctg ctgggtgctg cgcgcagcga gatcgcccgc
      241 cgcctcgaca tgatcgaccc gagtgcgtgg gcattcgtct gggtggtgga cccgccgttg
      301 ttcgagccgg ccgacgaggc cgcagcgcac ggtgacgtcg cggtgggttc aggtgcgtgg
      361 acagcggtgc accacgcgtt caccgcaccg aagccggagt gcgtcgacgc tatcgatgac
      421 gatccgggca gcgtgctggc tgatgcgtac gacatcgtct gcaacggcca cgagatcggt
      481 ggtggctcaa ttcgtatcca ccgccgcgac attcaggagc gcgtgttcgc ggtgatgggc
      541 atcgacaagg ccgaggccga ggagaagttt ggattcctgc tgaacgcctt cacttttggc
      601 gcaccgccgc acggcggcat tgcattcggc tgggaccgga tcacggcgct gctggccggg
      661 atggattcga tccgtgaggt gatcgcgttc ccgaagacgg gcgggggagt cgacccgctg
      721 accgatgcgc cgacaccgat cacgcctgcc cagctcaagg acgcgggcat cgccgtcatc
      781 gcacgaaagg atgagaagtg agcgacatgc ccgacccagc cgaactcaaa ggcttcaacg
      841 ccaagatcat tgaggagttt cgtaccaacg gcggcaaggt cggcggccag ttcgaaggcg
      901 cagacctgct gttgttgacg acgacgggcg cgaagtccgg gcagcccagg gtgagcccgc
      961 tggcgtatca gcgcgtcgac ggcaagatgc tgatcatcgg ctcgtaccga ggcgccgacg
     1021 tcgacccggc gtgggtgcac aacctgcggg ccaacccgca ggcgcacgtc gaagtcggca
     1081 gcgactccta cgacgtcgtc acgcgcgagc tgccgcccga ggagcgcgac caagcgtggg
     1141 acgagatcgt cgcggccgcg ccgggattcg gcgaatacca gtcgaacacc agccgcgtca
     1201 tcccgctctt cgaactcacg cgggcctgat cgcgccgagg cggtaaccac cgcggccctc
     1261 actcgaagtt tcccgcgcgg aataccgttt cgcggaggtc agctcggtga tccgcgttgg
     1321 ccacgccgcc gtcccacgcg ctagttcgtg aagcagtacg agtcgctgga cgcgtcacca
     1381 ccctgaagcc gggtctggtg cacccggagg ccacggaagt cgtcgccgtg cgggaagaag
     1441 cggccatcga ggaccagacc accggccgcg acgtccgcgt ccatcttcgc catccatgcc
     1501 ccgaccccgt ccgggtagaa gacctcatcc cacgccgcgt agagcgagtt ggtgacgtag
     1561 acgcgtcggc cgtcacggct gatctcgacc atttgcggcc cgccgccgag cggcaggccc
     1621 ggagccgcgg gatgcggctc gcgtcgcacg atccccccga ggcgcaccga tgccgtctcc
     1681 cgcgggtgga acgggtcgct gacgtcgtac tgcttgagtt cacctgtgcc aaagcaggat
     1741 acgtacagcc agcgatcgtc gaccgacaga tcgatgtcgg tgaccagtgg cgccgccgca
     1801 ccgaaaggct tcagcaacgg cggcaggtcg tcggcgtcgg cgggctcggc cggaatggtg
     1861 atcaccttct cgaccgacca ctggtcaccg tccttgtgcc acagccagac tgaggccgac
     1921 agatcctcga cgctgattac cactgacgcg aagccccacg cttgggacgg gtcgtgggcg
     1981 ggtcgcagtt cgagcaccat ctggtgttgg tcaccgaggt cgacgcgctg ggtcagtttg
     2041 cgctccgaca tgctccagaa gttcaagtgg tgcccgaact tccggccgag caggtcctcg
     2101 gggttgagcc cgttctcgat cattgcgggc gtcgcccact ccgacgtcat caccgtgtcg
     2161 tacttcaggt gccaccacac gtcgtaggcc aagaattgcg gtccccggtc gacctcccac
     2221 ggaccgatga cctcgaacgt gtcgtgatcg atcagcgcca cgccgccggg accgtcgtca
     2281 ccgttgacgc cgcccaaagc cgacatgaag atgccgcccg gtccgcagtg cacggtgtgc
     2341 ggacgcgagt atcccgcctt ggccgcgagc tcggcggcct tgatcgtgtg cgacagcacc
     2401 ggctgccgag ggtcgggctt ggtatccagg acatacgtgt tcgacgagcg aatgccgggc
     2461 acgaccaggt agcggcgctc gacggtcgca ccgtggtggc cggcgtgccc ctcgtggcag
     2521 agcgcactcg aacaggcgtt ccagccaaag tggtgtagtt cattgccggg cgtcgggaga
     2581 tcggtccagc cgacgacgct gccgaacgtt gcggagttgg ggtcgcagtc gaccacggcg
     2641 atcgcgtctt tgcgctggct tgccgggtcg aaggcggcca cataggcgag ttgctcgggt
     2701 ggggccgcga cagcggcgct gggactgcgg tagaaagtgg ggtctgtggt gggcatgaat
     2761 cctcctcgac tcgtgcccat cgatgatcct cgctccgcgc cgcccgcgtg gcggaattgt
     2821 ccgcaccccg aatcgaagac agtccacgtc gacgcaagca tgggcgtgtg acaacggacg
     2881 acgggaacat gtacggtgcg ccaccgggca gcggcatctc gatgccgcct tactacctgc
     2941 cgaccccgtc ggtgaagaac cgcaacaact acttccctca aagtgagcgg atcggcgacg
     3001 gcgagattcg gatcaccttt atgggcagca acccgtgggc gcctcggctg tcgcaggccg
     3061 ggacctgcac catggtggag ctcggcaccg caggacggtt gacgcaggtg aggtgtcacc
     3121 taatcgaaat ccctgctcat gcagagcatt tggtgtcact tgaacacact ccatgcgccc
     3181 aagaaggcgg atcgttcatg tgatttctcg aaacgggttc gtcgaatcgg gccagcgcta
     3241 cagtggatat aagcctcaca agtgagcatt tcgatccgcg acctgcgcac tttcgttgag
     3301 actcagaaag atcacaggaa gtgtgaacat ggcaaccatt cgattgacaa cacgccgcac
     3361 cgttgtcgcg ctgggcatcg ccgcggcgat tgcagccgcc ccagcggtcg cgacgttcgc
     3421 cgggtctccc catgtcctcg ccgaccccgg cgaatgtacg acaaccggcg agccgggtga
     3481 cgcttcgttg aactgcgcgc ccgccttcgt gcccgagatc ggcgcgccca gtgagatgga
     3541 actgaccgac accaacccgg gcataggttc tccggaacac agccaccact gacccagccc
     3601 gctaatgtgc ggtgagtgct gcacttgcgg gtgatcgcgc cgaccgagct gcgggagtcg
     3661 gtgatcgacg tcctgcgccg ggacaagggc gtgacccacg tcgtcgtgca ccgcaacgcc
     3721 gccctcgatc cgcccggcga cgaggtcacc gccgacatcg cacgtgaagc cgccaacgac
     3781 gtgatcgacg acctcaagga actcgggctg aaacggcgcg gcgccatcac gctcgacgtc
     3841 gtcgacacgg tcgtgtccac tgcggcctac cgcgccgaaa aggaggccga gggcgacccc
     3901 gccgacgcgg tgatctggga cgagctcgcc gccaggaccc gcgaagagtc gtcgctgaac
     3961 gtcacctacc tgacgtttct gtcgctggct tgcctgatcg ccgccgtcgg cgtggtgacg
     4021 gactcgccag tcacggtcgt cggcgcgatg gtggtcggcc cggagttcgg gccgctggcc
     4081 gcgctcgcgg tcgcgatcgt gcggcgccgc ggctacctcg cccggcgggc ggcgctggcg
     4141 ctgctggtcg gcttcccgct cgcgatgttc gtcacggcgg tcggcgtgct cgtcggtgag
     4201 gccgtcggct ggatcacgct ggccaccacg aagcagctcg aggaggtcga cttcatcttc
     4261 caggtcgggc cgctgtcgct cgtggtcgcg ctgttcgccg gcgcggcggg catgctgtcg
     4321 ctggtgtcgg cgaagtcgtc ggcgctggtc ggggtgttca tttcggtgac gacggtgccg
     4381 gccgccgggt tcgccgtggt ggcggccacg gtcggcgact gggacatcgc cgccaagtcc
     4441 gcgacacagc tcggggtcaa tctggccggg atcgtgttcg ccggcgtcct ggtgctgtgg
     4501 gtgtatctgc ggctgacctc ccaccaccgc gccgtgcgcc ggacctgagg cggcgctgcg
     4561 accagcttcc acctgtggtg ggattcccac cgtgggcatc aaggtggcgc tggagcatcg
     4621 caccagctac acgttcgacc ggctggtcga ggtgtacccg cacgtcgtgc ggctgcgccc
     4681 ggcgccgcac tcccgcaccc cgatcgaggc gtactcgctg accgtcgagc ccgccgacca
     4741 cttcatcaac tggcagcagg acgcgttcgg caacttcctg gcccggctgg ttttcccgaa
     4801 ccgtacccgg cagctgacga tcacagtcgg cctgatcgcc gacatgaagg tgatcaaccc
     4861 gttcgacttc ttcatcgaag actgggccga aacagtgcca tttgctcttc gcccgagtgg
     4921 tcccccgcaa gcgggaggtg cccccacatc gacgccattc gaatacccca aagcgatggc
     4981 cgaggacctg cagccatatc tgcggccggt cgacgaggac gacgacggct ccggccctgg
     5041 ggagctggtc cgaaaatggg tgagcaggtt cgaggtgccg aagggaaccc gcacgatcga
     5101 cttcctggtc gcgctcaacc gcgccgtcaa cgccgatgtc gcctacagcg tg
//
LOCUS       DASWBF010000164         6858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_865560,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000164 DASWBF010000000
VERSION     DASWBF010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865560"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..834)
                     /locus_tag="VF874_05520"
     CDS             complement(121..834)
                     /locus_tag="VF874_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX9175564.1"
                     /translation="MNAQPVSDALLRPVRPGNAFEDTVGRLLQTIRLGVIAPGESLPS
                     ERELAARLQVSRDTVREAIKSLAEAGYLVSRRGRYGGTFLADELPTAGADGAQGITRT
                     ELDDALRLREILEVGAARMAAARTLTAAEREPLWSRMTDVRGADPSDYRRLDSRLHLA
                     IAELVGSPSLVPLVAENRMRVNRLLDRIPLLSRNIAHSDEQHEAIVTAILAGDPDAAE
                     MAMRAHIAGSASLLHGFLD"
     gene            complement(831..1607)
                     /locus_tag="VF874_05525"
     CDS             complement(831..1607)
                     /locus_tag="VF874_05525"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX9175565.1"
                     /translation="MIDLSQRLTGKVAVITGGASGIGLATGRRMRAEGATIVVADVDR
                     VAGQEAADDLGGLFVPVDVTDESAVNALFDTAASTYGSVDIAFNNAGISPPDDDLIEN
                     TELAAWQRVQDVNLTSVYLCCRAALRHMTAANKGSIVNVASFVAVMGSATSQISYTAS
                     KGGVLAMSRELGVQHARSGIRVNALCPGPVNTPLLKELFAKDPERAARRLVHVPVGRF
                     AEPEEIAAAVAFLASDDASFITASTFLVDGGISAAYVTPI"
     gene            complement(1604..2980)
                     /locus_tag="VF874_05530"
     CDS             complement(1604..2980)
                     /locus_tag="VF874_05530"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9175566.1"
                     /translation="MTDVINPATEKVLRTVERMDTAGVDDAVARALAAQRHWAALPPA
                     ERAAALRAFAAAVDQHVDELAALEVANSGHPISSAEWEAGNVRDVLQYYAASPERMSG
                     KQIPVAGGLDVTFNEPLGVVGVITPWNFPMTIASWGFAPALAAGNAVLVKPAEWTPLT
                     TIRLGELAVEAGLPTDLLQVLPGKGSVVGERFVTHPDVRKIVFTGSTEVGTQVMAGAA
                     KQVKRVTLELGGKSANIVFDDCDLERAAATAPYGVFDNAGQDCCARSRILVQRNVYDQ
                     FVELLEPAVKGVVVGDPTSRDTEMGPLVSRTHWDTVASYVPDDAPVAFRGSAPSGPGF
                     WFPPTVLTPSRSDRTVREEIFGPVVTVLPFDDEADAIALANDTAYGLSGSIWTDNLSR
                     ALRVARAVESGNLSVNSHSSVRYNTPFGGFKQSGLGRELGPDAPLSFTETKNVFIAIE
                     DPAPGESS"
     gene            complement(2977..3711)
                     /locus_tag="VF874_05535"
     CDS             complement(2977..3711)
                     /locus_tag="VF874_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX9175567.1"
                     /translation="MSGYNPVLGLTTYLEQAQTGIWDVRASFLPHIYLEGVTLAGGIA
                     MLLPPQPVSDQIADEVLDRLDGLIITGGKDVDPARYGQAPHPLTDEPRRDRDAWEFAL
                     VAGALKRGMPVLGICRGAQVLNVALGGTLHQHLPDVVGHSGHRAGNAVFSTSRVHVVP
                     GTRLAALIGEYSDEECYHHQAIAELGEGLIVSARDEDGVIEAVERPGDTFIVAVQWHP
                     EERLNDLRVFAAVVEAAQAYAKERVA"
     gene            complement(3698..5047)
                     /locus_tag="VF874_05540"
     CDS             complement(3698..5047)
                     /locus_tag="VF874_05540"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX9175568.1"
                     /translation="MLTLRELEEEIVEGDIDTVIVAFADMQGRLTGKRVSSRLFIEEV
                     AKHGAECCNYLLAVDVDMNTVDGYVMSSWDTGYGDMVMTPDFTTLRRIPWLPGTALVM
                     ADLTWEDGRPVTAAPRSILARQIARLAERGLMPYVGTELEFMVFDNTYRDAWARGYRD
                     LTPATDYNVDYAMLGSTRMEPLLRDIRRGMIGAGMYCEGVKGECNRGQQEIAFRYDHA
                     LVTCDNHTIYKNGAKEIADKHGKSLTFMAKFDEREGNSCHIHISLRGKNGGAVFADPD
                     DKHQMSEMFRSFVAGQLATLRELTLFYAPNINSYKRFVEGSFAPTAIAWGMDNRTCAV
                     RVVGHGAGMRMECRAPGGDVNQYLAVAALIAGGLHGIDQQLELPEPLSGNAYESGAER
                     LPTTLAEAADLFAASAVAQEAFGDDVVEHYLNNARVELAAFNAAVTDWERVRGFERL"
     gene            5183..6760
                     /locus_tag="VF874_05545"
     CDS             5183..6760
                     /locus_tag="VF874_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9175569.1"
                     /translation="MPEGHEYLDEDEEILARLGYMQELQRSWSGFSNFAISFSIISIL
                     AGCFTSFGLGWNNGGPAAIAWGWPIVSAFILIIGLCLSELVSAYPTSGGIYWWASKLG
                     GPKAGYYTGWLNLIGLVAILASVSYGCATFLDLTLGTFSESWLNSYSLQRTFLLFVII
                     LAISAIINIFSSHLLAIINNISVWWHVAGAAAVVLILILIPEQHASVSDVFAKTINNT
                     GIFGGEKGWGWLLFVLPISAILTQYTITGYDASAHLSEETKSAANAAAKGLWQSIFYS
                     AIGGWILLLSFLFAVQDADDVSAKGGGVATIFTQAMDAKWVGIVLFISTAGQFFCTTA
                     CQTSASRMLFAFSRDRAVPGHQLWSKVSSNRVPANGVIVTAVSAALITLPALITVKVA
                     VGGEEVPSPVAFFAVVSIGVVGLYLCFAVPIYYRWKAGDEFAQGSWNLGRHWKWMAPI
                     AIAEIVVTSIIALFPYSNAGMPWDPNFEWKYVNYTPLLVGGTLLLLWIYWHVSVKKWF
                     TGPVRQIDEIAPEPEPA"
BASE COUNT         1115 a         2375 c         2173 g         1195 t
ORIGIN      
        1 cgcagcatgt ctggtaatgg gcagcataca tcggacacca acgcacggtg aggtatccgc
       61 gagcgtgcgc agaattacga cgctgcacgg cgtgtcgcgc agcaaacacg cacgctcgcg
      121 ctaatcgaga aagccgtgca gtagcgatgc ggaacctgcg atgtgcgcgc gcatcgccat
      181 ctctgcagcg tcggggtcgc cggccaagat cgcggtgaca atcgcctcgt gctgctcgtc
      241 ggagtgcgcg atgttacgtg acagcaacgg gattcggtcc agcagccggt tcacccgcat
      301 ccggttctcg gcgaccagcg gcaccagcga cggcgagccc accagctccg cgatcgccag
      361 gtgcagccgc gagtcaagcc gccggtagtc agacgggtcg gcgccgcgga cgtcggtcat
      421 cctcgaccac agcggctccc gctcggccgc cgtcagcgtg cgcgcggccg ccatgcgtgc
      481 cgcgccgacc tccaggatct cgcgcagccg cagcgcgtcg tcgagctctg tacgcgtaat
      541 gccttgcgcg ccatcggctc ccgctgtcgg gagctcgtcg gccaggaatg tgccgccgta
      601 gcggccacgc cgcgacacca gataccccgc ctcggccagc gacttgatcg cctcccggac
      661 ggtgtcgcgg ctcacctgaa gacgcgcggc gagctcacgt tccgacggca gtgactcgcc
      721 gggcgcgatg acgccgagcc ggatggtctg caacagccgc ccgaccgtgt cctcgaacgc
      781 gttgcccggc cgcaccggtc gcagcagcgc atcgctgacc ggttgagcgt tcatatcggc
      841 gtgacgtagg ccgcactgat tccgccgtcg acgaggaatg tcgatgcggt gatgaacgac
      901 gcgtcgtcgc tggccaagaa cgccaccgcg gcggcgatct cctcgggttc agcgaaccgg
      961 ccgacgggca cgtggaccag ccggcgcgcc gcgcgttcgg ggtccttggc gaacagctcc
     1021 ttgagcagcg gggtgttgac cggccccggg cacagggcgt tgacccggat tccgctgcgc
     1081 gcgtgctgca caccgagctc gcgcgacatc gccagcaccc cgcccttgga cgcggtgtag
     1141 gagatctgcg acgtggccga ccccatcacc gcgacgaacg acgccacgtt gacgatcgag
     1201 cccttattag cggcggtcat gtgccgcaac gccgcccggc agcacagata caccgacgtg
     1261 aggttgacgt cctgcacccg ctgccaggcc gccagctccg tgttctcgat caggtcgtcg
     1321 tcgggcggcg agatgcccgc gttgttgaaa gcgatgtcga cggaaccgta tgtgcttgcg
     1381 gccgtgtcga acagcgcgtt gaccgccgac tcgtcggtca cgtcgacggg tacgaacagc
     1441 cccccgagat cgtcggcggc ctcctgtccc gcgacccggt cgacgtcggc cacgacgatc
     1501 gtcgcgccct ccgcgcgcat gcgccggccg gtcgccagcc cgatcccgct cgcgccgccg
     1561 gtgatcaccg cgaccttgcc ggtcaggcgc tgcgaaagat cgatcatgac gactcccctg
     1621 gcgctggatc ttctatggcg atgaacacgt tcttggtttc ggtgaacgac agcggcgcgt
     1681 caggacccag ttcacggccg aggcccgatt gcttgaaacc gccgaacggc gtgttgtatc
     1741 gcaccgacga gtgcgaattc accgacagat tacccgattc caccgcccgc gccacgcgca
     1801 gcgcgcgcga caggttgtcg gtccagatcg accccgacaa cccgtaagcc gtgtcgttgg
     1861 ccagcgcgat cgcgtccgcc tcgtcgtcga acggcaacac cgtgacgaca ggcccgaaga
     1921 tctcctcccg caccgtgcga tccgaccgcg aaggcgtcaa cacggttggc ggaaaccaaa
     1981 atcccggacc cgacggggcg ctgccgcgaa acgcgaccgg cgcgtcgtca ggtacgtacg
     2041 aggccaccgt gtcccagtgc gtccgcgata ccagcggacc catctcggtg tcccgtgatg
     2101 tcgggtcgcc gacgaccacg cccttaacgg cgggttccag caactccacg aattggtcgt
     2161 agacgttgcg ctgcaccagg atccggctgc gcgcgcagca atcctggccg gcgttgtcga
     2221 acaccccgta cggggcggtc gcggcggccc gttccagatc gcagtcgtcg aacacgatgt
     2281 tggcgctctt gccgccgagt tcgagggtga cgcgcttgac ctgctttgcg gcaccggcca
     2341 tcacctgggt gcccacctcc gtcgacccgg tgaacacgat cttgcgcacg tcaggatggg
     2401 tgacgaaccg ctcgcccacc accgagccct taccgggcag cacctgcagc aggtcggtgg
     2461 gcaatcctgc ctcaaccgcc aactcgccca gccggatcgt cgtcaacggc gtccattccg
     2521 cgggcttcac cagcacggcg ttgccggccg cgagcgcagg cgcaaacccc cacgacgcga
     2581 tcgtcatcgg gaaattccac ggcgtgatca ccccgacgac gccgagcggc tcgttgaacg
     2641 tgacgtccag accgccggca accggaatct gcttgcccga catgcgctcc gggctcgcgg
     2701 cgtagtactg gagcacatcg cggacgttgc cggcctccca ctcggccgac gagatcgggt
     2761 ggccggagtt cgcgacctcc agcgcggcca gttcgtcgac gtgctggtcc accgccgccg
     2821 cgaacgcgcg cagcgcagcc gcccgctccg cgggcggtaa agcggcccaa tgcctttgcg
     2881 cggccagcgc ccgcgccacc gcgtcgtcga cacccgcggt gtccatccgc tcgacggtcc
     2941 gcagcacctt ctcggtcgcc gggttgatca cgtcggtcat gcgacgcgtt ccttcgcgta
     3001 ggcctgagcc gcttcgacga ccgccgcgaa cacccgcaag tcgttgagcc gctcctccgg
     3061 atgccactgc acggcgacaa tgaaagtgtc gcccggcctt tcgacggcct cgatcacccc
     3121 gtcctcgtcc cgtgcgctga cgatcaggcc ctcgcccaac tcggcgatcg cctgatggtg
     3181 gtagcactcc tcgtcggaat actcgccgat caatgccgct agtcgagttc cgggcacgac
     3241 gtgcacacgt gacgtggaga acaccgcgtt gcccgcgcga tgcccgctgt gtccgacgac
     3301 gtccggcaga tgctggtgca gcgtgccgcc caacgccacg ttgagcacct gcgcgccgcg
     3361 gcagatgccc agcaccggca ttccccgctt caaagcgccg gctaccaaag cgaattccca
     3421 cgcgtcgcgg tcgcggcgcg gttcgtcggt cagcggatgc ggcgcctgcc cgtagcgggc
     3481 cgggtcgacg tccttgccgc cggtgatgat cagtccgtcg agtcggtcga gcacctcgtc
     3541 ggcaatctgg tcggagaccg gctggggcgg cagcagcatc gcgatcccgc cggccagcgt
     3601 cacgccctcc aggtagatgt gcggcaggaa gcttgctcgc acgtcccata ttccggtctg
     3661 cgcctgttcc aggtacgtcg tcaagccgag caccgggtta tagccgctca aagccacgca
     3721 ccctctccca gtcggtgacg gcggcgttga acgcggccag ttcgactcgc gcgttgttca
     3781 ggtagtgctc gacgacgtcg tcgccgaagg cctcctgcgc gactgccgac gcggcgaaca
     3841 agtccgcggc ttcggccagc gtcgtcggca gccgctcggc gccgctctca taggcgttgc
     3901 cggacaatgg ttctggcagt tccagctgct ggtcgatgcc gtgcaggccg ccggcgatca
     3961 acgcggcgac cgccagatac tggttgacgt cgccgccggg cgcccggcac tccatccgca
     4021 tcccagcgcc gtggccgacc acccgcaccg cgcacgtgcg gttgtccatg ccccacgcga
     4081 tcgccgtcgg cgcgaagctg ccttcgacaa aacgcttgta ggagttgatg ttcggtgcgt
     4141 agaacagcgt cagttcgcgc agcgtcgcca gctgtccggc cacgaaactg cggaacatct
     4201 ccgacatctg gtgcttgtcg tcggggtcgg cgaacacagc gccaccattc ttgccgcgca
     4261 gcgagatgtg gatgtggcag ctgttgccct cccgctcgtc gaacttggcc atgaacgtca
     4321 ggctcttgcc gtgcttgtcg gcgatctcct tcgcgccgtt cttgtagatc gtgtggttgt
     4381 cacacgtgac cagcgcgtgg tcgtagcgga acgcgatctc ctgctgcccg cgattgcact
     4441 cgcccttgac gccctcgcag tacatacccg cgccgatcat gccgcgccga atgtcacgca
     4501 gcagcggctc cattcgcgtc gacccgagca tcgcgtagtc gacgttgtag tccgtggccg
     4561 gcgtgaggtc gcgatagccg cgcgcccagg cgtcccggta ggtgttgtcg aaaaccatga
     4621 actcaagctc ggtgcccacg tacggcatca gtccgcgctc ggcgagccgg gcgatctggc
     4681 gggccaggat gctgcgcggc gcggcggtga ccgggcggcc gtcttcccac gtcaagtcgg
     4741 ccatcaccag cgcggtgccg ggcagccacg ggatccgccg caatgtcgtg aaatcgggcg
     4801 tcatcaccat gtcgccgtag ccggtgtccc agctgctcat cacatacccg tcgacggtgt
     4861 tcatgtcgac gtcgaccgcg agcaggtagt tgcaacactc ggcgccgtgc ttggccacct
     4921 cctcgatgaa cagccgcgat gacacgcgtt tgccggtcag ccggccctgc atgtcggcga
     4981 acgcgacgat caccgtgtcg atgtcgccct cgacgatctc ttcctcgagc tcccgcaagg
     5041 tcaacatggc taattgggca cccctccgtc agcacgtcgc gtgtcgattt aaccgaaccg
     5101 tcattgccaa aggtaggact tcagaccttt gacgttctat tgtcccctac gttggcgcgc
     5161 tcgcacgtct gaaggagaca ccgtgcccga gggtcatgaa tacctggacg aggacgagga
     5221 aattctcgcg aggctcggct acatgcagga acttcaacgc tcctggtcgg gcttctccaa
     5281 cttcgcgatc tcgttctcga tcatctcgat cctcgccggg tgtttcacgt cgttcgggct
     5341 gggctggaac aacggcggcc ccgccgcgat cgcgtggggc tggccgatcg tgtcggcgtt
     5401 catcctgatc atcggcctgt gcctgtccga gttggtctcc gcatacccga cctccggcgg
     5461 aatctactgg tgggcaagca aactcggcgg tccaaaggcc ggctactaca ccggctggct
     5521 gaacctgatc gggctggtcg cgatcctggc ctcggtgtcg tacgggtgcg cgacgttcct
     5581 cgatctcacc ctgggcacgt tcagcgagag ctggctcaac agctacagcc ttcagcggac
     5641 attcctgctg ttcgtgatca tcctggcgat ctcggcgatc atcaacatct tctccagcca
     5701 cctgctggcg atcatcaaca acatctcggt gtggtggcac gtggcgggcg cggccgcggt
     5761 ggtactgatt ctgatcctga tacccgagca gcacgcgagc gtcagcgacg tgttcgccaa
     5821 gaccatcaac aacaccggca tcttcggcgg cgaaaagggc tggggctggc tgcttttcgt
     5881 cctaccgatc tctgcgatcc tgacccagta cacgatcacc ggctacgacg cgtccgcgca
     5941 cctctccgag gagaccaaga gcgccgccaa cgcggccgcc aagggcttgt ggcagtcgat
     6001 cttctattcg gcgatcggcg gctggatcct gttgctgtcg tttcttttcg ccgtccagga
     6061 cgccgacgac gtgtcagcga agggcggcgg agtcgccacg atcttcacgc aggcaatgga
     6121 cgccaagtgg gtggggatcg tgctgttcat ctccaccgcg ggccagttct tctgcaccac
     6181 cgcctgccag accagcgcgt cgcggatgtt gttcgcgttc agccgcgatc gcgccgtacc
     6241 cggacatcag ctgtggtcga aggtcagctc gaacagggtt cccgccaatg gggtgatcgt
     6301 gacggcggtc agcgcagcac tcatcacgct gcccgccttg atcacggtga aagtcgcggt
     6361 cgggggtgaa gaggtcccgt cgcctgtcgc gttcttcgcg gtggtgtcga tcggcgtcgt
     6421 cggcctctac ctgtgcttcg cggtgccgat ctactaccgc tggaaggccg gtgacgaatt
     6481 cgcccaggga agctggaatc tcggcaggca ctggaagtgg atggcgccga tcgcgatcgc
     6541 cgagatcgtc gtcacctcga tcatcgcgct gttcccgtac agcaacgccg gtatgccatg
     6601 ggacccgaac ttcgagtgga agtacgtcaa ctacacaccg ttgctggtcg gcggcacgct
     6661 gttgctgctg tggatctact ggcacgtgtc ggtgaagaag tggttcaccg gcccggtccg
     6721 gcagatcgac gagatcgcgc cagagccgga gcctgcgtaa cgagcaggcg agcgatgtag
     6781 gcgtgcggga gcgttccagc cacctctcgc cgagactgca ttttgacagc cgaacttcga
     6841 gtcggggcct gccggaat
//
LOCUS       DASWBF010000165        22728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_740551,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000165 DASWBF010000000
VERSION     DASWBF010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22728
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740551"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VF874_05550"
     CDS             <1..300
                     /locus_tag="VF874_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9175570.1"
                     /translation="RDLDEAYQKVDVLVSPATPTTAFRLGEKVDDPLAMYLFDLCTLP
                     LNLAGHAGMSVPSGLSPDDSLPVGLQIMAPALADDRLYRVGAAYEAARGPLPAAI"
     gene            408..1439
                     /locus_tag="VF874_05555"
     CDS             408..1439
                     /locus_tag="VF874_05555"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX9175571.1"
                     /translation="MRIGILTGGGDCPGLNAVIRAVVRTCDSRYGSSVVGFQDGWRGL
                     LEDRRIQLQNDDRNDRLLAKGGTMLGTARVHPDKLRAGLDQIKQTLDDNGIDVLIPIG
                     GEGTLTAAHWLSEENVPVIGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAES
                     HQRVMLVEVMGRHAGWIALNCGLASGAHMTLIPEQPFDVEEVCRLVKRRFQRGASHFI
                     CVVAEGAKPAAGSMKLAEGGIDEFGHEKFTGVAAQLAHEVEKRINKEVRVTVLGHVQR
                     GGTPTAYDRVLATRFGVNAADAAHVGEYGMMVSLRGQDIGRVALADATRQLKLVPQSR
                     YDDAAAFFG"
     gene            complement(1463..2110)
                     /locus_tag="VF874_05560"
     CDS             complement(1463..2110)
                     /locus_tag="VF874_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9175572.1"
                     /translation="MTSPPRRTYRSTLRAAQARQTRHAIVSAAGRLFARDGFGATTID
                     AIAEDAGVSRKTVFTAVGGKIELLKIALDWAIAGDDEPLAVADREPVSRLMHQDDPAA
                     LLRGWAKVLAEIDHRVAALVRAMEIAASLDPAARRLFKQAQTQRLQGARAVVDRLVDL
                     TALNSALTADEAADIAWLFADPALYDWLVRQRGWPLDRFAQWLGDALCRQLLAQG"
     gene            2178..3500
                     /locus_tag="VF874_05565"
     CDS             2178..3500
                     /locus_tag="VF874_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9175573.1"
                     /translation="MIIGAGPAGLFTAIALARRGRDVVVVDRDRGPAPHSPFGWDRKG
                     VMQFHHAHTFRGQVVDALQVEMPDVLRRLVAYGASVATTPGTTRPTALLSRRILFEQV
                     LRQTASVEPGITLVAGHADRVVRERGRAVGIQVDGRTLRAELVIDASGRASRVTSDIR
                     LPAVVHPCGAAYVSRQYQLRAHGEAGPVNSPIGLSLSFNGYSAIVFVHDNRTFSAVLI
                     YDGADRQLRKLRRATVFEAALSAIPALNDWVDPDRSFPITGVLPGGRLYNTYRGQLAE
                     SGRPAAVGMISIGDSVCTTTPLAGRGVAMALMQSRALIRLLDEHGSDHHSCAIAFDQW
                     CFDNVKPWFDDHTVADTERLRRWAGDDVDLTRRLPSDLIVAAGEVDPKLRALVAPFVT
                     MDALPPSLSTAEPRARQIYASGWRPPIPAGPSLDELAELCGRKSLATV"
     gene            3541..5046
                     /gene="gatB"
                     /locus_tag="VF874_05570"
     CDS             3541..5046
                     /gene="gatB"
                     /locus_tag="VF874_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX9175574.1"
                     /translation="MTLAPSAELLDYDEVVTRYEPVLGLEVHVELSTATKMFCGCPTA
                     FGAEPNTQVCPVCLGLPGALPVLNEAAVESAIRIGLALNCEIVDWCRFARKNYFYPDM
                     PKNYQISQYAEPITVNGYLDVPLDDGTTFRVGIERVHMEEDTGKLTHLGSETGRIAGA
                     TESLLDFNRAGVPLIEIVTKPVVGAGERAPEIARAYVTALRDLLRALGVSDVRMDQGS
                     MRCDANVSLMPIGATEFGIRTETKNVNSLKSVEVAVRYEMRRQAAILASSRRVHQETR
                     HFHEDGYTSPGRSKESAEDYRYFPEPDLEPVAPSTELVERLRNTLPELPWLQRKRIQQ
                     DWSVSDEVMRDLVNAGAVELVAATVEQGASSEAARAWWGNFLVQKANEAGVPLDALPI
                     TPAQVAEVVKLVDDGKLSNKLARQVVEGVLAGEGEPQQVMKARGLELVRDDSALQAAV
                     DEALAANPDVVEKIRGGKVAAAGAIVGAVMKATKGQADAARVRELVLAACS"
     gene            complement(5043..6164)
                     /locus_tag="VF874_05575"
     CDS             complement(5043..6164)
                     /locus_tag="VF874_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9175575.1"
                     /translation="MRMRGGVRVVLSALFAAVLLATGCARFDDAASAPFTTEPELSPP
                     PSSTPPPPPPLPDNPFPKQCPAPGVMQGCLESTSGLIMLPDSKSALVAERTTGAVKEV
                     SVSAEPKVKLVIPVDPSGDGGLMDIVLSPTYQQDRLMYAYISTPTDNRVIRIADGDVP
                     KDILTGIPKGATGNTGALIFTSPTTLVVLTGDAGNPAMAADPNSLAGKVIRIEQPTTV
                     NQAPITTAMSNMGSGGGLCTDPTDGSLYITDRTPTADRLQRITADSKTSTVWTWPDKP
                     GVAGCAALAGTILVNLVNTKQTVAVRMAPETGAVTGEPEVMRQDEHGHAWALAVSPDG
                     NIWGATVNKTFGDAMQLDDVVFPLFKTGGGFPRPKDDLV"
     gene            6313..7140
                     /locus_tag="VF874_05580"
     CDS             6313..7140
                     /locus_tag="VF874_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9175576.1"
                     /translation="MHSHDSRPWRRPNDPAPSSRPASARLVDPEDDLPSATYGGDFET
                     TTIPHYNSDDSSRSGLSLLAEPEPLPYLDPSTSGRHDVLPGTVDYDDEVDERVAAANR
                     RGTQDLGLLLLRASVGAILIAHGLAKAFGLWGGQGLDGFRNSLADMGYEHAGLLTYLA
                     AGTQISAGVLLVLGLFTPLAAAAALAYLINGLVASVAAQYDAGYIGFFLPDGNEYQLL
                     LIAAVASIILVGPGRYGLDAGRGWARRPFLGSFAALLLGIGVGVGAYMLLNGGNPLS"
     gene            complement(7180..7611)
                     /locus_tag="VF874_05585"
     CDS             complement(7180..7611)
                     /locus_tag="VF874_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX9175577.1"
                     /translation="MSTGESEHSEAAPVVLRMSPTAHLASGFAALGMLAVVLAVPMTA
                     PLLVIPVIASVMIVRLRTVADGQTVTARTLLGSRTVDWEDIKGLRFVKGKLARAELTS
                     GEDLRLPAVTFATLPLLAEVSGGRVPNPYTREAADSQEKVE"
     gene            7839..9701
                     /locus_tag="VF874_05590"
     CDS             7839..9701
                     /locus_tag="VF874_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX9175578.1"
                     /translation="MSAPTTRPSEWSAPEAQDAGPANGGAATGHQPGHPKRIAPQHLT
                     GAQAVVRSLEELGVDVIFGIPGGAVLPVYDPLFDSQKLRHVLVRHEQGAGHAASGYAH
                     ATGRVGVCMATSGPGATNLVTPIADAQMDSIPVVAITGQVGRPMIGTDAFQEADISGI
                     TMPITKHNYLVRNGDDIARVIAEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPTME
                     LPGYKPTTKPHAKQIRAAAKLIAAARKPILYVGGGVIRGDASAQLLDLAELTGIPIVT
                     TLMARGAFPDSHPQHLGMPGMHGTVAAVAGLQRSDLIIGLGARFDDRVTGKLASFAPE
                     AKVIHADIDPAEIGKNRNADVPIVGDIKAVITDLTDALRKTAHGRERLDDWWEYLRGV
                     QNTYPLSYGPQSDGSLSPEYVIETLGKIAGSDAVYVAGVGQHQMWAAQFISYEKPRTW
                     LNSGGLGTMGYAVPAAMGAKLARPDAEVWAIDGDGCFQMTNQELATCAIEGAPIKVAI
                     INNGNLGMVRQWQSLFYDERYSQTDLATHSHRIPDFVKLAEALGCVGLRCEREEDVVD
                     VINQARTINDRPVVIDFIVGADAQVWPMVAAGTGNDEIQAAQGIRPLFDTEDLV"
     gene            9701..10204
                     /gene="ilvN"
                     /locus_tag="VF874_05595"
     CDS             9701..10204
                     /gene="ilvN"
                     /locus_tag="VF874_05595"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX9175579.1"
                     /translation="MTVSTHTLSVLVEDKPGVLARVASLFSRRGFNIQSLAVGATEQK
                     NLSRMTIVVTVEDLPLEQVSKQLHKLVNVIKVVEQENENSVDRELALIKVRADAGTRG
                     QVIEAVNLFRAKVIDVSTESLIIESTGTQEKIEALLRVLEPYGVREIVQSGVVSMSRG
                     PRSITQR"
     gene            10242..11243
                     /gene="ilvC"
                     /locus_tag="VF874_05600"
     CDS             10242..11243
                     /gene="ilvC"
                     /locus_tag="VF874_05600"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX9175580.1"
                     /translation="MFYDDDADLSIIQGRKVGVIGYGSQGHAHSLSLRDSGVDVKVGL
                     REGSKSRDKVAEQGLEVDTPTEVAKWADVIMLLAPDTAQADIFKNEIEPHLEDGNALF
                     FGHGLNIHFGLIKPPANVTVGMVAPKGPGHLVRRQFVDGKGVPCLVAVEQDPKGEGEA
                     LALSYAKGIGGTRAGVIKTTFKDETETDLFGEQAVLCGGTEELVKTGFDVMVEAGYAP
                     ELAYFEVLHELKLIVDLMYEGGIARMNYSVSDTAEFGGYLSGPRIIDAATKERMKQIL
                     NEIQDGTFVKKLVANVEGGGKELEELRKRNAEHPIEVTGKKLRDLMSWVDRPITETA"
     gene            complement(11419..12006)
                     /locus_tag="VF874_05605"
                     /pseudo
     CDS             complement(11419..12006)
                     /locus_tag="VF874_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(12111..12295)
                     /locus_tag="VF874_05610"
                     /pseudo
     CDS             complement(12111..12295)
                     /locus_tag="VF874_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12292..13725)
                     /locus_tag="VF874_05615"
     CDS             complement(12292..13725)
                     /locus_tag="VF874_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9175581.1"
                     /translation="MDVTVVGSGPNGLAAAVVCARAGLSVQVLEAQPTLGGGARTLPD
                     PEYPGVRHDICSAVHPLALGSPFFAEFDLAARGVGLTVPEISYANPFVERPAAIAYRD
                     LDRTAEGLDDGKSWRRLLEPMVQRADGVVDFLLGDKRSLPTDPVLLLQLGVRMAWQGT
                     PAWNATFEGRDAEALFTGVAAHTISPLPSITSAAAGMLLASLAHSVGWPIPVGGSQAI
                     ADALIADLRAHGGVLHSDTEVTEPPAGVVLFDTAPTALLRIYGSRLPRRYANRLRRYR
                     FGSGVAKVDFVLSDDVPWSDARLASAVTFHLGGDREDMAYAEAQVAAGRHAERPMVLA
                     ALPHLADPGRVDDHGRRPFWTYAHVPAGSTVDQTETVTNVVESFAPGFRDVVVAVRSV
                     PAARMTGHNANYVGGDIAVGGNNAWRALAGPVARLNPWTTPLPGVYLCSSATPPGAGV
                     HGMSGYYAARTMLRREFVIDRPPGLAP"
     gene            13757..15343
                     /gene="serA"
                     /locus_tag="VF874_05620"
     CDS             13757..15343
                     /gene="serA"
                     /locus_tag="VF874_05620"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9175582.1"
                     /translation="MSLPVVLIADKLAESTVAALGDQVEVRWVDGPNREKLLAAVPEA
                     DALLVRSATTVDAEVLNAGTKLKIVARAGVGLDNVDVDAATARGVLVVNAPTSNIHSA
                     AEHAVALLLAAARQIPAADASLRERTWKRSAFSGTEIFGKTVGVVGLGRIGQLVAQRV
                     GAFGARVVAYDPYASHARAAQLGIELLSLDELLGVADFISVHLPKTQETAGLIGKEAL
                     AKTKPGVIIVNAARGGLIDETALADAIASGHVRGAGLDVFAKEPCTDSPLFDLPQVVV
                     TPHLGASTAEAQDRAGTDVAASVKLALAGEFVPDAVNVGAGAVSEEVAPWLDLVRKLG
                     LLAGVLSTEPTATLSVRVCGELASEDVEVLRLSALRGLFATVTDQQVTFVNAPALAAE
                     RGVQSEISTASESLNHRSVVDVRAVAADGSVVNVAGTLSGPQEVQKIAQINGRNFDLR
                     AEGTNLIIHYGDRPGTLGKIGTLLGAADINIHAAQLSEDAEGPFATIMLRLDRDVPAD
                     LRSAIAEAVGATLLEVVDLS"
     gene            15340..16350
                     /locus_tag="VF874_05625"
     CDS             15340..16350
                     /locus_tag="VF874_05625"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX9175583.1"
                     /translation="MKLAIIAGDGIGPEVIGEAVRVLDAVVPGIEKTSYDLGARRYHA
                     TGEVLPESVLDELRGHDAILLGAIGDPSVPSGVLERGLLLKTRFALDHHINLRPARLY
                     PGVASPLAGNPQIDFVVVREGTEGPYTGTGGAIRVGTPHEIATEVSVNTAYGVRRVVQ
                     DAFQRAAARRKHLTLVHKNNVLTFAGDLWWRTVQEVGQEYPDVEVAYQHIDAATIHLV
                     TDPGRFDVIVTDNLFGDIITDLAAAVCGGIGLAASGNIDATHVNPSMFEPVHGSAPDI
                     AGKGVADPTAAVMSVALLLEHAGEPEAAARVDKAVEQHLATRGDTTFSTTDVGERILA
                     RL"
     gene            16433..17401
                     /locus_tag="VF874_05630"
     CDS             16433..17401
                     /locus_tag="VF874_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HEX9175584.1"
                     /translation="MTQLDAGTIRDAVELACHAPSLHNSQPWKWVTHGPVLELFAVPD
                     PQVYSTDRSGRELLLSCGALLDHLRVAMAAAGSHTTVQRLPDADDPHHLATCHFERSA
                     AEPAHRERADAILRRRTDRLPFAAPRGWQATEAVLRSAAGRSDVTLDVIPDDSRQQLA
                     EASRLTEDIRRHDPTYQSELHWWTSPFEFDQGLPPSSRVSESEAARVDIARSFPTTGY
                     GQRRPQVASDRSKVIVLSTRDDTADEVLRCGEALSAVLLECTLAGLATCTLTHMIEVT
                     PARELIRRLVGQSGQPQLLVRVGTPVGHQPPATPRRPVSEVLEFRE"
     gene            complement(17406..18410)
                     /locus_tag="VF874_05635"
     CDS             complement(17406..18410)
                     /locus_tag="VF874_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX9175585.1"
                     /translation="MDLSTRYLGLQLRNPLLASASPLSHTVDGVRRLADAGVGAVVLY
                     SLFEEEIRREAQQQARLAMEYNETYAESLTYFPATVNQDHGARRYLHLVESAASSVDV
                     PVIGSLNGATPGGWARYARSIQDAGAAAIELNIYYLPGDAHISGRDVEQRHLDVLAGV
                     KAAVSVPVAVKLSPFFSSTAAMAHQLDEAGADGLVLFNRFLQPDIDCESMTPAPGVVL
                     SSAAEIRLPLTWISLLRGRLRASLAATTGVESATEVVKYLLAGADVVQSASALLRHGP
                     QYAGTLLDGLRAWMRRKEYATVDEFRGMLAVPEDGSMRERRDYVAAVRDANSRTYHVY
                     "
     gene            complement(18410..21982)
                     /gene="nifJ"
                     /locus_tag="VF874_05640"
     CDS             complement(18410..21982)
                     /gene="nifJ"
                     /locus_tag="VF874_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX9175586.1"
                     /translation="MTRTTIDGNEAAVSVAYRLNEVCCIYPITPSSPMAELADEWSSR
                     QRPNVWGTVPTVVEMQSEGGAAGALHGSLQAGALTTTFTSSQGLLLMIPNMYKIAGEL
                     TSAVIHVAARAIAAQGLSIFGDHQDVMAVRQTGFALLSSASVQEAHDLALVAQAVTLR
                     TRVPFLHFFDGFRTSHELNTIEMLADEDLRALIPEELVREHRARGLSPERPFIRGTAQ
                     NPDIYFQARETVNPFYARVPAVVEEMMAALAERTGRRMHIVDYTGHPEAERVLVLMGS
                     GAQTAAQTVAALTARGERVGVVQLRLYRPFPTEALLAALPSTARTVAVLDRTKEPGSI
                     GEPLFLDVVAALTDAYCRGERSAMPRVCGGRYGLSSKEFTPAMVAGVFDELAREQPRP
                     RFTVGIDDDVSGSSIGYDPAFDIESPDTVRAVFFGVGSDGTVGANKNTIKILGAEENL
                     YAQGYFVYDSKKSGSQTVSHLRFGPEPIKAPYLLAQADFVGCHHSRFLAAADVVGCAA
                     PGAALLLNSRRPPDKVWDSLPRPVQQQIIDKGVRVYAVDAGRIASDVGLAGRINVVLQ
                     TCFFAISEVLPRDKAIVRINEAVTKAYGKHGEDVLNRELAAVDRALDGLRRVEVPDRV
                     TATYEPGPVVPVTAPEFVRTVTAEMIAGRGDALPVSALPVDGTYPSGTAAYEKRNISE
                     LVAVWDPDTCIQCGNCTFVCPHSVIRTKFYEQDQLGGAPETFPSVPLSAVGLPDTRYT
                     LQVYAEDCTGCGLCVEVCPVINPGAPLTKAINLGPREPIVDSSRENIAFFETLPFANR
                     SRVDFGNVRGSQFLEPLFEFPGACAGCGETPYLKLLSQLFGDRLMIANATGCSSIYGG
                     NLPTTPWTHNAMGRGPAWSNSLFEDNAEFGLGFRLAADTHTRQARGLLTAHRDVLGAE
                     LVDETLTAPQQYESELRAQRDRVAELEARLDRLDPAVADELRSVIDHLVRRSVWIVGG
                     DGWAYDIGAGGLDHVLASGRNVNVLVLDTEVYSNTGGQASKSTPLGAVAKFAAGGKTT
                     PRKDLALQMIAYGNVYVARVAMGADGQQTLQAFREAEAYDGPSLVIAYSHCIAHGFDM
                     RDGLDQQYRAVNSGHWPLVRYDPLLRTAGKSPFLLDSHRPRLSLTDYRYRELRFRSLA
                     NSHPEEAERLLALAQESVDRRWKTYEDMASQGADDFEPDARKAP"
     gene            complement(21979..>22728)
                     /locus_tag="VF874_05645"
     CDS             complement(21979..>22728)
                     /locus_tag="VF874_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9175587.1"
                     /translation="EEEAQDAEREGVQIHWLRTITAFDRHEMQVEVMELDDNGKPRPT
                     GRFETLAADTVIMALGQETESAFMRTLPGVDFDDDGNVLVSPTLMTGYPGVFAGGDMV
                     PCERTVTVGVGHGKKAAHHIDAWLRDTSDERPPKHPVATFDKLHLWYFGDAARRAQPE
                     LPPDEAVAGFEEVVGGLTAQAAAYEAGRCLSCGNCFECDGCFGSCPEDAINKVPMGHQ
                     GYEFNYDKCTGCGTCFDQCPVHAIEMIPEPR"
BASE COUNT         3826 a         7513 c         7736 g         3653 t
ORIGIN      
        1 cgcgatctcg acgaggcgta ccagaaggtc gacgtgctgg tctcgccggc gacacccacc
       61 accgcgttcc ggctgggaga gaaggtcgac gacccgctgg cgatgtacct gttcgacctg
      121 tgcacgctgc cgctcaacct cgccgggcac gctggcatgt cggtgccgtc cgggctgtcg
      181 cccgacgact cgctgccagt cgggctgcag atcatggcac ccgcgttggc tgacgaccga
      241 ctgtaccggg tgggcgccgc ctacgaggcc gcccgcgggc cactgccggc cgcgatctga
      301 ccggcttcct cgttaacgcg agcagacgca caagccccca aaattgccgc ttgtgggtga
      361 ttttgcgtct gctcgcgggg aaagggtgca gagggcaaac taggcccatg cgcatcggaa
      421 tactcaccgg cggcggcgac tgtccaggct tgaacgcggt gattcgcgcg gtggtacgca
      481 cgtgcgactc ccgctacggc tcgtcggtgg tcggcttcca ggacggctgg cgcgggctgc
      541 tggaagaccg gcggatccag ctgcagaacg acgatcgcaa cgaccgcctg ctggccaagg
      601 gcggcacgat gctcggcacc gcccgcgtgc atcccgacaa gctgcgcgct gggctggacc
      661 agatcaagca aacgctggac gacaacggca tcgacgtgct gatcccgatc ggcggcgagg
      721 gcacgctgac ggccgcgcac tggctgtcgg aggagaacgt ccccgtgatc ggggtgccga
      781 agaccatcga caacgacatc gactgcaccg acgtgacgtt cgggcacgac accgcgctga
      841 ccgtcgccac cgaggccatc gaccggctgc actccacggc cgagtcgcac cagcgggtga
      901 tgctcgtcga ggtgatgggc cgccacgccg gctggatcgc actgaactgc gggctcgcct
      961 ccggcgctca catgaccctg attcccgagc agccgttcga cgtcgaagag gtgtgccggc
     1021 tggtcaagcg gcggttccag cgtggggcgt cgcacttcat ctgcgtggtg gccgagggtg
     1081 ccaaacccgc ggcgggttcg atgaagctgg ccgaaggcgg catcgacgaa ttcggccacg
     1141 agaagttcac cggggtggcc gcgcagctgg cgcacgaggt ggagaagcgg atcaacaagg
     1201 aagtgcgggt gaccgtgctg ggtcacgttc aacgcggcgg cacaccgacg gcctacgacc
     1261 gtgtgctggc cacccgattc ggggtcaacg ccgccgacgc cgcacatgtg ggcgagtacg
     1321 ggatgatggt gtcgctgcgg ggacaggaca tcggtcgggt ggcgctggcg gacgccacgc
     1381 gccagctcaa gctggtgccg cagagccgct acgacgacgc ggcggcgttc ttcggctgag
     1441 cgagccttgg cgagcgagac aactagccct gagcgagtag ctgtcggcac aacgcgtcac
     1501 ccagccactg cgcgaatcgg tccagcggcc agccgcgctg gcgcacgagc cagtcgtaca
     1561 gcgcgggatc ggcgaaaagc caagcgatat cggccgcctc gtcggccgtg agcgcactgt
     1621 tcagcgcggt caagtccacc agacgatcga cgacagcgcg ggcgccctgc agacgttgag
     1681 tctgcgcctg cttgaagagc cttcgagcag cggggtcgag gctggcagcg atttccatcg
     1741 cgcgcacgag cgccgcgact cggtggtcga tctcagccag caccttcgcc cacccgcgta
     1801 gcagcgccgc cggatcgtcc tgatgcatca gccggctgac cggttcgcgg tcggccaccg
     1861 ctaggggctc gtcgtcgccg gcgatcgccc aatccagcgc gatcttgagt aactcgatct
     1921 ttccgcccac cgccgtgaaa accgtcttgc ggctcacacc tgcgtcctcg gcgatggcgt
     1981 cgatcgtggt cgcaccgaag ccgtcgcggg cgaacaggcg gcctgccgcg gacacgatcg
     2041 cgtgtcgcgt ctgacgggct tgggccgctc tcagcgtgga ccggtaggtc cggcgagggg
     2101 ggcttgtcat ttcaagtaac ccatagtgta atcattaaca tagaggatac ctcaatctgg
     2161 agcagacatt gcgaactgtg atcatcggtg ccggtccggc cgggctgttc accgccatcg
     2221 cgttggctcg ccgcgggcgg gacgtcgtcg tggtggatcg agatcgtggg cccgcgcctc
     2281 actcgccgtt tggttgggat cgcaagggcg tcatgcagtt tcaccatgcc cacaccttcc
     2341 gcggccaggt cgtcgacgcg cttcaggtcg aaatgcccga tgtcctgaga cggttggtcg
     2401 catacggcgc ctcggtggcg acgacgccgg gcacgacgcg tccgacggct ctgctgagcc
     2461 gcagaatcct tttcgagcag gtgctgcggc agacggcatc ggtcgaaccg ggcatcacgc
     2521 tcgtcgcggg gcacgcggac cgggttgtgc gtgagcgagg ccgggctgtc ggtatccagg
     2581 tcgacggtcg gacgctgcgc gcggagctcg tcatcgacgc gtccgggcgg gccagcaggg
     2641 tcacctcgga catccggctc ccggccgtcg tgcatccgtg cggagcggcg tacgtgagcc
     2701 gccaatacca gctgcgtgct cacggcgagg cggggccggt caattcgccg atcggtctct
     2761 cgttgagttt caatggctac agcgccatcg tctttgtgca cgacaaccgg acattctcgg
     2821 cggtcctgat ctatgacgga gccgaccggc agctgcgcaa gctgcgtcgc gcgaccgtgt
     2881 tcgaggcggc tctgagcgcg attccggcgc tcaatgactg ggtcgatccg gaccggtcgt
     2941 ttccgatcac gggcgtgctg ccaggcggca ggctctacaa cacctaccga gggcaactgg
     3001 cggagtcggg ccgcccggcg gcagtcggaa tgatctcgat cggcgactcg gtttgcacca
     3061 caacgccgtt ggcgggtcgt ggtgtcgcga tggcgttgat gcaatcgcgc gcgctcatcc
     3121 ggctgctcga cgaacacggg agcgaccatc actcatgcgc cattgcgttc gaccaatggt
     3181 gtttcgacaa cgtcaagcca tggttcgacg accacaccgt cgccgatacc gaacgtctgc
     3241 gacggtgggc gggcgacgac gtcgacctca cccgccgact gccctcggat ctcatcgtcg
     3301 cagccggtga ggttgatccg aagctgcgag cgctcgtcgc cccgttcgta acgatggacg
     3361 cgcttccacc aagcctctcg acggccgaac cccgagccag gcagatctac gcctccggct
     3421 ggcgaccgcc aattcctgcg ggccccagtc tcgacgagct cgcggagtta tgcgggcgga
     3481 aaagcctggc gacggtgtga cggtgatctt gtcggcgacg gcccaattac catcgacggc
     3541 atgaccctgg ctccatcggc ggaactactc gactacgacg aggtcgtcac gcgctacgag
     3601 ccggtgctcg ggctcgaggt gcatgtcgag ctgtcgaccg ccacaaagat gttctgcggg
     3661 tgtcccactg cgttcggggc tgaaccgaac acgcaggtgt gcccggtctg cttgggcctg
     3721 cccggggcgc tgccggtgct caacgaggcc gcggtggagt cggcgatccg cattgggctc
     3781 gcgctgaatt gcgagatcgt ggactggtgc cggttcgccc ggaagaacta cttctacccg
     3841 gacatgccga agaactacca gatttcgcaa tacgccgagc cgatcaccgt caacgggtat
     3901 ctcgacgtgc cgcttgacga cgggaccacg ttccgggtcg ggatcgagcg ggtacatatg
     3961 gaggaagaca ccggcaagct cacacacctg ggcagcgaga cagggcgcat tgcgggagcg
     4021 acggaatcgc tgctggattt caaccgggcc ggtgtgccgc tgatcgaaat cgtcacaaag
     4081 ccggtcgtcg gcgccggtga gcgcgcaccg gagatcgccc gcgcctacgt caccgcgctg
     4141 cgagatctgt tgcgggcatt gggcgtttcc gatgtacgaa tggatcaggg ctcgatgcgc
     4201 tgcgacgcca acgtgtcact gatgccgatc ggggccacgg agttcggcat ccgtaccgag
     4261 acgaagaacg tgaactcgct gaaaagcgtc gaggtggcgg tgcgctacga aatgcgtagg
     4321 caggcagcga ttcttgcctc cagtcggcga gtgcaccaag agacgcggca ctttcacgag
     4381 gacggctaca cgtctccggg tcgcagcaag gaatccgcgg aggattaccg ctatttcccc
     4441 gaacccgatc tcgagccggt cgccccgagc accgaactcg tggagcggtt gcgcaacaca
     4501 ctgcccgaac ttccgtggtt gcagcgcaag cggattcagc aggactggag tgtctcggac
     4561 gaggtgatgc gcgacctggt caacgcgggc gcggtggagt tggtagccgc aactgtggag
     4621 cagggcgcct caagtgaagc tgcccgggca tggtggggca acttcctcgt gcagaaggcc
     4681 aacgaagccg gtgtgccgct cgacgcgttg ccgatcacac cggcacaggt cgctgaggtg
     4741 gtcaagctcg tcgacgacgg caagctgtcg aacaagctgg cccgtcaggt ggtcgagggc
     4801 gtgctggccg gcgagggcga accgcagcag gtgatgaagg cacgtgggct ggagttggtg
     4861 cgcgacgact cggcgcttca ggccgcggtc gacgaggcgt tggccgcgaa cccggacgtg
     4921 gtcgagaaga ttcgtggcgg caaggtggct gccgcgggag cgatcgtcgg cgcggtgatg
     4981 aaggcgacca aggggcaagc cgacgccgcg cgggtgcgcg agttggtgtt ggccgcctgc
     5041 agttagacca ggtcgtcctt cggccgcggg aagccgccac cggtcttgaa cagcgggaac
     5101 accacgtcgt cgagctgcat cgcgtctccg aacgtcttgt tgacggtggc gccccagatg
     5161 ttgccgtcgg gagacacggc cagcgcccag gcatgtccgt gctcgtcctg acgcatcacc
     5221 tccggttcac cggtcaccgc tccggtttcg ggtgccatcc gcaccgcgac ggtctgcttg
     5281 gtgttgacga gattcaccag gatggtgccg gcgagcgccg cacagccggc cacccccggc
     5341 ttgtccggcc acgtccacac ggtcgaggtc ttggaatcgg cggtgatccg ctgaagtcgg
     5401 tccgcggtcg gcgtgcggtc ggtgatgtag agcgagccgt cggtggggtc agtgcacagg
     5461 ccgccgccgg agcccatgtt ggacatcgcg gtggtgatcg gcgcctggtt gacggtggtg
     5521 ggctgctcga tccgaatgac tttgcccgca agggaattgg ggtcagctgc cattgccggg
     5581 ttgcctgcat cgccggtgag gacgaccagc gtcgtggggc tggtgaagat cagcgcgccg
     5641 gtgttgcctg tggcgccctt cgggattccg gtgaggatgt ccttggggac gtcgccgtcg
     5701 gcgatgcgga tgacgcggtt gtcggtgggt gtgctgatgt atgcgtacat cagccggtcc
     5761 tgctgatagg tcggcgacaa cacgatgtcc atcaggccgc cgtcgcctga cgggtccacc
     5821 gggatgacga gcttgacctt gggttcggcg ctgaccgaca cctctttcac cgcaccggtg
     5881 gtgcgctcgg ccaccaaagc ggacttgctg tccggcagca tgatcagccc gctggtgctt
     5941 tccaggcagc cctgcatcac tcccggggcc gggcactgct tcgggaacgg gttgtccgga
     6001 agcgggggag gcggcggcgg ggtcgaactc ggcggcggcg acaactccgg ctcggtggtg
     6061 aacggcgccg acgccgcgtc gtcgaaccgc gcgcagccgg tggccagcag cacggcggcg
     6121 aacagcgccg acagcaccac ccgcactcct ccgcgcatcc gcatgcccgc caggttacgg
     6181 atagttgggc cgacgccgcc acgccgacac cggccgcggg gcgaccctcg cttggcttcg
     6241 gaccggtaaa ggtgcggtga cggcccggca caaatccgcg gttcgggggt tacttgccct
     6301 taccctggca caatgcactc gcatgactcg cgcccgtggc gacggcccaa cgatccggcc
     6361 ccgtcctcgc gccccgcttc ggcacgcctg gtcgatcccg aggatgacct gccgtctgcg
     6421 acttacggcg gcgacttcga aaccaccacg attccgcact acaactccga cgactccagc
     6481 cggtctgggc tgagcctgct cgcggagccc gaaccgctgc cctacctcga cccgagcacg
     6541 tcaggccgtc atgacgtgct gcctgggacc gtcgactatg acgacgaagt cgatgagcgg
     6601 gtcgccgcgg ccaaccggcg cggcacccaa gacctcgggc tgctgctgct gcgcgcgtcg
     6661 gtcggcgcca tcctgatcgc gcacgggctg gccaaggcct tcggcttgtg gggtggtcag
     6721 gggctcgacg gcttcaggaa ctcgctcgcc gacatggggt acgagcacgc cggcctgctg
     6781 acgtacctgg cggccggcac gcagatctcg gccggcgtgc tgctggtcct gggtttgttc
     6841 acgccactgg ccgcagcggc cgcgctggcg tacctgatca acgggctggt tgccagcgtc
     6901 gcggcccaat acgacgcggg ctacatcggg ttctttcttc ccgacggcaa cgagtaccag
     6961 cttctgctga tcgcggcggt ggcctcgatc attctcgtcg ggccgggccg ctacggcctg
     7021 gacgcgggcc gaggctgggc gcggcggccg ttcctcggct ccttcgcggc gctgctgctc
     7081 ggtatcggcg tgggggtagg cgcctacatg ctgctcaacg gcggcaatcc gctcagttaa
     7141 ccgggcaccg aagcgccgaa actgtattct ggcaggccgt tactcgactt tttcctgcga
     7201 atctgcagcc tcgcgcgtgt atgggttggg cacccggccg ccgctaacct cggccagcag
     7261 cggcaacgtc gcgaacgtca ccgcgggcag ccgcaagtcc tcgcccgatg tcagctcggc
     7321 gcgcgccaat ttacctttga cgaaccgcag acccttgatg tcctcccaat cgacggtgcg
     7381 gctgcccaac agcgtgcgcg cggtgaccgt ctgcccgtcg gccactgtcc gcagtcgcac
     7441 gatcatcacc gacgcgatca ccgggatgac cagtagcggc gccgtcatcg ggacggcaag
     7501 cacgacggcc aacatgccca gtgcggcgaa gcccgacgcc aggtgcgccg tcggcgacat
     7561 acgcagcaca acgggcgcgg cctcggagtg ttccgactcg ccggtgctca tacaggcatc
     7621 attccaccca cccggaattt gacggttgac cagtgcggaa gctaccgtcg gaggctatgc
     7681 agccccgtgg aatgctcgta gtaattggta ggcgcgttgg tgaatagccc ggcatagcca
     7741 gcaaccaccg acgcgcaacc ctcgtgcagc agccgacgct gacgagggtt ttttcttgcc
     7801 ctagaccacg aagagacaaa agagacaaag aggacaccgt gagcgcaccc accacgcgac
     7861 catccgaatg gtctgcgccg gaagctcaag acgctgggcc cgccaacggc ggagccgcca
     7921 ccggtcacca gccgggtcac ccgaaaagaa ttgcgccaca acaccttacc ggtgcacagg
     7981 ccgtcgtccg gtcgctggag gaactcggcg tcgacgtgat cttcggaatc cccggcggcg
     8041 cagtgcttcc ggtgtacgac ccgctgttcg actcgcagaa gctgcgtcac gtgctggtgc
     8101 gccatgagca gggcgcgggg cacgccgcca gcggctacgc gcacgccacc ggccgcgtcg
     8161 gcgtctgcat ggcgacgtcc ggtccggggg ccaccaatct cgtcaccccg atcgccgacg
     8221 cgcagatgga ctccataccc gtcgtcgcga tcaccggaca ggtcgggagg ccgatgatcg
     8281 gcaccgacgc gttccaggag gccgacatct ccggcatcac gatgccgatc accaagcaca
     8341 actaccttgt ccgcaacggt gacgacatcg cgcgggtgat cgccgaggcg tttcacatcg
     8401 ccgcctcggg acggcccggc gcggtgctcg tcgacatccc gaaggacgtg ctgcagggcc
     8461 agtgcacgtt cagctggccc cccacgatgg agctgcccgg ctacaagccc accacgaaac
     8521 cgcacgccaa acagatccgt gcggcggcca agctgatcgc ggccgcccgc aagccgatcc
     8581 tgtacgtcgg cggcggcgtg atccgcggcg acgccagcgc gcagctgctc gacctggccg
     8641 agctgaccgg aatcccgatc gtcacgacgc tgatggcacg cggcgcgttt cctgacagcc
     8701 acccccagca cctcggcatg ccgggcatgc acggcaccgt cgccgcggtg gccgggctgc
     8761 agcgcagcga cctgatcatc gggctcggcg cccggttcga cgaccgcgtc accggcaagc
     8821 tggcgtcctt cgcgccggag gccaaggtga tccacgccga catcgacccc gccgagatcg
     8881 ggaagaaccg caacgccgac gtgccgatcg tcggcgacat caaggccgtc atcaccgacc
     8941 tgaccgacgc gctgcgcaag accgcgcacg ggcgcgagcg cctcgacgac tggtgggagt
     9001 acctgcgggg ggtgcagaac acctacccgc tgagctacgg cccgcagagc gacggcagcc
     9061 tcagcccgga gtacgtgatc gagacgctgg gcaagatcgc tggctcggac gcggtgtacg
     9121 tcgccggcgt cggtcagcac cagatgtggg ccgcgcagtt catctcctac gagaagccgc
     9181 gcacctggct caactccggc gggctcggca cgatgggcta cgcggtcccg gcggcgatgg
     9241 gcgccaagct tgcccgtccg gacgccgagg tgtgggcgat cgacggcgac ggctgcttcc
     9301 agatgaccaa ccaggaactg gccacgtgcg caatcgaggg tgctccgatc aaggttgcga
     9361 tcatcaacaa cggcaacctc gggatggtgc gtcagtggca gtcgttgttc tacgacgagc
     9421 gctacagcca gacggatctg gccacccact cgcaccgcat cccggatttt gtgaagctgg
     9481 ccgaggcgct gggttgtgtc ggattgcgtt gcgagcgtga ggaagacgtg gtcgacgtga
     9541 tcaaccaggc gcggacgatc aacgaccggc cggtggtgat cgacttcatc gtcggggccg
     9601 atgcgcaggt gtggccgatg gtcgcggcag ggacaggcaa cgacgaaatc caggcagcgc
     9661 aggggatccg gccgctgttc gacacggagg acctcgtctg atgactgtct ccacgcacac
     9721 gctctcggtg ctcgtcgaag acaagcccgg cgtcctggcc cgtgtcgcgt cgttgttctc
     9781 gcggcgcggg ttcaacatcc agtcgctggc tgtcggggcg accgagcaga agaacttgtc
     9841 gcggatgacg atcgtggtca ccgtcgagga cctgccgctc gagcaggtca gcaaacagct
     9901 ccacaagctg gtcaacgtaa tcaaggtggt cgagcaggaa aacgagaact cggtcgaccg
     9961 cgagctggcg ctgatcaagg tgcgcgccga cgcgggcacc cgcggccagg tgatcgaggc
    10021 ggtgaatctg tttcgcgcca aggtcattga cgtctccacc gagtcgctga tcatcgagtc
    10081 caccggcacg caggaaaaga tcgaggcgct gctgagggtg ctcgagccct acggcgtccg
    10141 cgagatcgtt cagtcgggcg tggtgtcgat gagccgtggc ccacgtagca tcacccagcg
    10201 gtaagcgaac agaaaaggga agattcactg tggcagttga gatgttctac gacgacgacg
    10261 cagacctgtc gatcattcag ggccgcaagg tcggcgtcat cggctatggc agccagggcc
    10321 acgcgcactc gctgagcctg cgcgactccg gcgttgacgt gaaagtcgga ctgcgcgagg
    10381 ggtccaagtc gcgggacaag gtcgccgagc agggcctcga ggtcgacacg cccacggagg
    10441 tggcgaagtg ggccgacgtg atcatgcttc tggcgcccga caccgcgcag gcggacatct
    10501 tcaagaacga gatcgagccg cacctcgaag acggcaacgc gctgttcttc gggcacgggc
    10561 tcaacatcca cttcgggctg atcaagccgc cggcgaacgt gaccgtcggc atggtggcgc
    10621 cgaaagggcc gggacacctg gtgcgccgcc agttcgtcga cggcaagggt gtgccgtgtc
    10681 ttgttgcggt ggaacaggat ccgaagggcg aaggcgaggc gctggcgctg tcgtatgcga
    10741 agggtatcgg cggcacccgc gccggcgtca tcaagaccac gttcaaggac gagaccgaaa
    10801 ccgacctgtt cggcgagcag gccgtgttgt gtgggggcac agaggaactc gtcaagaccg
    10861 ggttcgacgt gatggtcgag gccgggtatg cgccggaact ggcgtacttc gaggtgctgc
    10921 atgagctcaa gctgatcgtc gacctgatgt acgagggcgg catcgcgcgg atgaactact
    10981 cggtgtcgga caccgccgag ttcggcggct acctgtccgg cccgcggatc atcgacgcgg
    11041 ccaccaagga gcggatgaag cagatcctga acgagattca ggacggcacg ttcgtcaaga
    11101 agctcgtggc caacgtcgag ggcggcggaa aggaactcga ggagctgcgc aagcgcaacg
    11161 ccgagcaccc gatcgaggtc accggcaaga agctgcgcga cctgatgagc tgggtggacc
    11221 gcccgatcac cgaaaccgcg tagtgtgtct gcgctctcat ctcacgcgcg ctgaccgctt
    11281 ctcagcgcgc cgaaatgcgc ttacggtgcg aagctcggca ggactttgat gctgaactcg
    11341 cgcctaacga tccgggttcg ggttcagggc catcgcaaga aagtcgaggc gccacaacga
    11401 ctcaaatagt tggcgcccga gtaacgagat cgtttctttg accatttgga ccgatcgctc
    11461 gtcttgactc agacccaagt cacgaaagac agactgcagc agattgactc cggttcggcc
    11521 gtactttgca tagagcatca aacctattac gccatcgggc cgcacgcatc cggcgagcgc
    11581 tttcatgcct gccagcggat ctgccatgtg atgtagaacg ccggttgaca caatgaggtc
    11641 gaagtcgagg ccgagcgttg tcagctcttc gatcggaagc agatgcaact ctaggttctg
    11701 cagtccgtgc ttgtctttga ggtattgctg atggtttagc gacgattggc tgatgtcgac
    11761 cgcatgaatt ttcgctgtgc ggttcgtgta cgcaaagacg gccgcctggt tggtaccgca
    11821 acccgcaatc aaaatgtcga ggtcgggttt gtattcacga tccggccata aaattcggtg
    11881 gcagtgcctc gggtcgaagc cttcttggtt gttactgagc catatctcga ggtcctggat
    11941 agggtcaggg tacgtccacc gctcgtactg gcggttcaca acatcggtgc gtgggtcgtc
    12001 gttcatctca gcgggcggct ccttggtgac tgaacgcgtc aattccatca tgcgaagcgc
    12061 gcccgcactg ccaccacacg cgggccggca gacagcgacg acgtgaacag cgacgatgtg
    12121 aaactgtcat agaccccact tgccctgtgc ccaaacgcgc cgagggagtc gatgatcgtg
    12181 cggaactgcg aactgacggg caggtacttc gtcgcttcgt agttggcgga tcgcgctggg
    12241 ccagcgtcgt accgtgtccc gtggcggagt agtagatgat cgcaagcttg gtcatggcgc
    12301 aagaccaggc ggccggtcga taacgaactc gcgccgcagc atcgtgcgtg cggcgtaata
    12361 gcctgacatc ccgtgtacgc cggcacccgg cggagtcgcc gacgagcaca ggtacacccc
    12421 gggaagcggt gtggtccacg ggttgaggcg ggcaacgggt ccagcgagag cccgccatgc
    12481 gttgttgcca ccgaccgcga tgtcaccgcc gacgtagttg gcgttgtgcc ccgtcattcg
    12541 cgcggcgggc acgctgcgga cggcgacgac gacgtcgcgg aatcccggtg cgaaactctc
    12601 gacgacgttg gtgacggtct ccgtctggtc gaccgtcgac cccgccggca catgtgcgta
    12661 tgtccaaaac ggccgccggc catggtcgtc gacccgtccc ggatccgcga ggtggggaag
    12721 cgcggccagc accatcggcc gctcggcatg ccgccccgca gcgacctgag cctcggcgta
    12781 cgccatgtcc tcgcgatcac cgccgaggtg aaacgtgacc gccgacgcca gccgcgcgtc
    12841 cgaccacggc acgtcgtctg acagcacgaa atccaccttc gccacaccgg agccaaagcg
    12901 atagcgccgc aaccggtttg cataacgccg tggcagccga gagccgtaga tccgcaacag
    12961 cgcggtcggt gccgtgtcga acagcacgac cccggcaggc ggctcggtga cttcggtatc
    13021 gctgtgcagc acgccgccgt gcgcgcgcag gtccgcgatc aaggcgtccg caattgcctg
    13081 actgcccccg accggtatcg gccagccgac ggagtgcgcg aggctggcca acagcattcc
    13141 cgccgccgct gacgtgatcg acggcagcgg tgagatcgta tgtgccgcaa cgccggtgaa
    13201 cagcgcctcc gcgtcgcggc cttcgaacgt cgcgttccac gctggtgtgc cctgccacgc
    13261 catccgcacg ccgagttgga gcagcaacac cggatccgtg ggcagcgacc gcttgtcgcc
    13321 gagcaggaag tccaccacac cgtcggcgcg ctgcaccatc ggttcgagca gcctgcgcca
    13381 cgacttgccg tcgtcgaggc cctccgcggt gcggtcgagg tcgcggtagg cgattgcggc
    13441 ggggcgttcg acgaacgggt tggcgtacga gatctccggc acggtcaacc ccacgccgcg
    13501 ggcggcgagg tcgaactcgg cgaagaacgg cgacccgagg gccaacgggt gcacggccga
    13561 gcagatgtcg tggcgcacgc cggggtattc gggatcgggc agcgtgcggg ccccgccgcc
    13621 gagcgtcggc tgcgcctcga gcacctgcac cgacaaccct gcgcgggcgc agacaacggc
    13681 tgccgccagc ccgttgggac cactgccgac cacggtgacg tccacgccgt aagtagaccc
    13741 cattaggctg gcccgcgtga gtctgcccgt tgtgttgatc gccgacaaac tcgccgaatc
    13801 caccgttgcc gcgctcgggg atcaggtcga ggtccgctgg gtcgacggac ccaaccgtga
    13861 aaagctgctg gccgcggtgc ccgaagccga tgcgctgctg gtgcgctcgg ccaccacggt
    13921 cgacgctgaa gtgctcaatg ccggcacgaa actcaagatc gtggcccgtg ccggcgtcgg
    13981 cctggacaac gtcgacgtcg acgccgccac cgcgcgcggc gtcctcgtcg tgaacgcgcc
    14041 gacctccaac attcacagtg ccgccgagca cgccgtcgcg ctgctgctgg ccgccgcacg
    14101 ccagatcccg gccgccgacg cctcgctgcg cgagcgcacc tggaagcgct cggcgttctc
    14161 cgggacggag atcttcggca aaaccgtcgg cgtggtgggg ctcggccgga tcggccagct
    14221 cgtcgcccag cgggtgggcg cgttcggtgc ccgcgtggtg gcctacgacc cgtacgcgtc
    14281 gcacgcgcgc gccgcgcaac tcggcatcga gctgctgagc ctcgacgagc tgctgggcgt
    14341 ggccgacttc atttcggtgc acctgcccaa gacgcaggag accgccgggc tgatcggcaa
    14401 ggaggcgctg gccaagacca agccaggcgt gatcatcgtc aacgccgcgc gcggtgggct
    14461 gatcgacgag accgcgctgg ccgacgcgat cgccagcggg cacgtgcgcg gcgcgggcct
    14521 cgacgtgttc gcgaaggagc cgtgcacgga cagcccattg ttcgatctgc cgcaggtcgt
    14581 cgtgacgccg catctcgggg cgtcgacggc agaggcgcag gaccgcgcgg gcaccgacgt
    14641 ggcggccagc gtaaagctgg cgctggcagg cgaattcgtg cccgacgccg tcaacgtcgg
    14701 agctggtgcc gtcagtgagg aagtcgcgcc gtggctggat ctggtgcgca agctcgggct
    14761 gctggccggc gtgttgtcga ccgagcccac cgcgacgctg tccgtgcggg tgtgcggtga
    14821 gttggcttcg gaagacgttg aagtgcttcg gctttcggcc ctgcgcgggc tgttcgcgac
    14881 ggtgaccgac cagcaggtga cgttcgtgaa cgcgccggca ctggccgccg agcggggagt
    14941 gcaatccgag atcagcaccg cctcggagag tctgaatcac cgcagtgtcg tcgacgtgcg
    15001 agcggtcgcc gccgacggct cggtggtcaa tgttgcgggc acgttgtccg ggccccaaga
    15061 ggtgcagaag atcgcgcaga tcaacggccg caacttcgat ctgcgcgccg agggcaccaa
    15121 cctgatcatc cactacggcg atcggcccgg gacgctgggc aagatcggca cgctgctggg
    15181 cgccgccgac atcaacatcc acgccgcgca gctgagcgag gacgcagaag gtcccttcgc
    15241 gacgatcatg ctgcgcttgg accgcgacgt gcccgccgac ctacgctcgg cgatcgccga
    15301 ggcggtcggc gccactctgc tggaagtcgt ggacctgtcg tgaagctggc gattatcgcc
    15361 ggcgacggaa tcggcccgga ggtcatcggc gaggccgtaa gagtgctcga cgcggtcgtc
    15421 cccggaatcg agaagacgag ctacgacctg ggggcgcgcc gctatcacgc gacgggcgag
    15481 gtgctgccgg agtcggtgct cgacgagttg cgcggccacg acgcgatcct gttgggcgcg
    15541 atcggcgacc cgtcggtgcc cagcggggtg ctggagcggg gactgctgtt gaagactcgc
    15601 ttcgccctgg atcaccacat caacttgcgg cccgcccggc tatatcccgg tgtggcaagc
    15661 ccgttggccg gcaatccgca gatcgacttc gtcgtcgtcc gcgaggggac cgagggcccc
    15721 tacacaggca ccggcggcgc gatccgggtc ggcaccccgc acgagatcgc caccgaggtc
    15781 agcgtcaaca ccgcctacgg ggtgcgaagg gtggtgcagg acgcgtttca gcgggccgct
    15841 gctcgtcgta aacatctgac gctggtgcac aagaacaatg tgctgacgtt cgcgggcgac
    15901 ctgtggtggc ggacggtgca ggaggtcgga caggagtacc ccgacgtcga ggtcgcgtac
    15961 cagcacatcg acgccgcgac gattcacctc gtcaccgatc ccggccggtt cgacgtgatc
    16021 gtcaccgaca acttgttcgg cgacatcatc acggacttgg cggccgccgt gtgcggtggc
    16081 atcgggctgg cggccagcgg caacatcgac gccacccacg tgaacccgtc gatgttcgaa
    16141 ccggtgcacg gcagcgcccc tgacatcgcg ggcaaggggg tagccgatcc gaccgcggcg
    16201 gtgatgtcgg tcgcgctgtt gcttgagcat gccggcgaac cggaagccgc tgcgcgggtg
    16261 gacaaagcgg tcgagcagca ccttgccact cgcggcgaca ccacgttctc cacgaccgac
    16321 gtgggggaac gaattctggc gcggctctag ggtcgccgag agcacgctta ttgcacggtt
    16381 tttcgtgatt tcggtgcaac aatcgtgctc tcggcgtcag gatggcgagg atatgacaca
    16441 gcttgacgcc ggaaccatcc gggatgcggt cgaacttgcc tgccatgcgc cgtcattgca
    16501 caacagccag ccgtggaagt gggtaaccca cgggccggtg ctcgagctgt tcgccgtccc
    16561 cgacccgcag gtgtattcca ccgacaggtc gggcagggaa ctgctgctga gttgtggagc
    16621 gctcctggac cacctgcgcg tcgcgatggc cgcggcaggc tcgcacacca ccgtgcagcg
    16681 acttcccgac gccgacgacc cgcatcatct ggccacgtgc cactttgagc ggtcggcggc
    16741 cgaacccgcg caccgcgagc gggccgatgc gatcctgcga cgccgcaccg accggctgcc
    16801 cttcgccgcc ccacgcggtt ggcaggccac cgaggcggtg ctgcgatccg ccgcgggccg
    16861 cagcgacgtg acgcttgatg tgattcccga cgattctcgg cagcagttgg ccgaggcatc
    16921 ccggctgacc gaggacatac gacgtcacga tccgacgtat cagtccgaat tacattggtg
    16981 gacatcgccg ttcgagttcg atcaaggtct gccgccgagc tcacgggtgt cggagtcgga
    17041 ggctgcccgg gtggacatcg cccggtcgtt cccgacaacc gggtacgggc agcgccgccc
    17101 gcaagtggca tcggaccgct ccaaggtcat cgtgttgtcc actcgtgacg acactgccga
    17161 cgaggtgttg cgctgcggcg aggcgttgtc ggcggtgttg ctcgagtgca ccctcgcggg
    17221 cctggccacc tgcacgctca cccacatgat cgaagtgacg cccgcccgcg agctgatccg
    17281 ccggctggtc ggccaaagcg gccaaccgca gctgttggtg cgggtcggca cgcccgtggg
    17341 ccatcaacct cccgcgacac cgcgacggcc ggtgagcgaa gtcctcgagt tccgggagta
    17401 aggcctcaat acacgtggta cgtgcggctg ttcgcgtcac ggaccgcggc cacgtagtcg
    17461 cggcgttccc gcatcgaccc gtcctcgggc acggcgagca tcccgcggaa ctcgtcgaca
    17521 gtcgcatact ccttgcgccg catccacgcg cgcagcccgt ccagcagcgt gccagcgtac
    17581 tgcgggccgt ggcgcagcag cgctgaggcc gactgcacca cgtcagcgcc cgcgagcaga
    17641 tacttgacca cctcggtggc gctctcgacg ccggtcgtgg cggccaacga cgcccgcagc
    17701 cgtccccgca gcagtgagat ccacgtcagc ggcaaccgga tctccgcggc gctggacagc
    17761 accacgcccg gggccggggt catggactcg cagtcgatgt caggctgcag gaaacggttg
    17821 aacagcacga gcccgtcagc ccccgcctcg tcgagctgat gcgccatcgc ggcggtcgaa
    17881 ctgaagaacg ggctcagctt gacggcgacc ggcacgctga ccgcagcctt gacgccggcc
    17941 aacacgtcga gatggcgttg ctcgacgtcg cggccgctga tgtgcgcgtc accgggcagg
    18001 tagtagatgt tcagctcgat ggcggccgca cccgcatcct ggatcgaccg cgcataccgt
    18061 gcccagccgc ccggcgtggc accgttgagg ctcccgatca ccgggacgtc gaccgacgac
    18121 gcggcgctct caactagatg gaggtagcgc ctggcgccgt gatcctggtt caccgtcgcc
    18181 ggaaaatacg tcaaggattc ggcgtaggtt tcgttgtact ccatcgcaag ccgagcctgc
    18241 tgttgcgctt cgcggcggat ctcttcctcg aacagcgagt acagcaccac cgcgccgacg
    18301 ccggcgtcgg cgaggcggcg caccccgtcg acggtgtgac tcagcggaga cgcactggcc
    18361 agcagcgggt tgcgcagctg cagtccgagg tagcgggtcg acaggtccat cagggggcct
    18421 ttcgggcatc gggctcgaag tcgtcggcac cctgagatgc catgtcctcg taggttttcc
    18481 accgacgatc gaccgactcc tgagccagcg cgagcagccg ctcggcctcc tccggatggg
    18541 agttggcaag actgcggaac cgcaactccc ggtagcggta gtcggtcagc gagagccgcg
    18601 gtcggtgtga gtccagcagg aacggtgact ttcccgcggt gcggagcagc gggtcgtagc
    18661 gtactagcgg ccagtgcccg ctgttgacgg cgcggtactg ctggtccagt ccgtcgcgca
    18721 tgtcgaaccc gtgggcgatg cagtggctgt aggcgatcac cagggacggc ccgtcgtagg
    18781 cttcggcttc ccggaacgcc tgcagcgtct gctgcccgtc ggcgcccatc gccacccggg
    18841 cgacgtagac attgccgtat gcgatcatct gcagcgcaag gtccttgcgc ggcgtcgtct
    18901 ttccgccagc ggcgaacttg gcgaccgccc ccagcggtgt ggacttcgag gcctgcccac
    18961 cggtgttcga gtacacttcg gtgtcgagca cgagaacgtt gacattgcgg ccgctggcca
    19021 gcacatggtc caagccgccg gcgccgatgt cgtaggccca gccgtcaccg cccacgatcc
    19081 acacactgcg gcgcaccagg tggtcgatca cgctgcgtag ctcgtcggcc actgcagggt
    19141 cgagccggtc cagacgggcc tccagttccg ccacccggtc gcgctgtgcc cgcagctcgg
    19201 actcatattg ctgtggggca gtgagggttt cgtcgaccag ctcggcgccg agcacgtcgc
    19261 gatgcgcagt gagcaacccg cgggcctggc gggtgtgggt gtcggccgcg agtcggaaac
    19321 cgaggccgaa ctcggcgttg tcctcgaaca gcgaattcga ccacgccggt ccgcgcccca
    19381 tcgcgttgtg ggtccacggg gtcgtcggca ggttgccgcc gtagatcgac gaacagcccg
    19441 tcgcattcgc gatcatcaac cggtcgccga acaactgcga cagcagcttc agatacggcg
    19501 tctcaccgca ccccgcgcat gcgccgggga actcgaacag cggctcgagg aactggctgc
    19561 cgcgcacgtt gccaaagtcc acccgggagc ggttggcgaa cggcaacgtt tcgaagaacg
    19621 cgatgttctc gcggctcgaa tccacgatcg gctcgcgggg accgaggttg atcgccttgg
    19681 tgagcggggc gcccggattg atcaccgggc acacttcgac gcacagcccg cacccggtgc
    19741 agtcctcggc atacacctgc agcgtgtaac gggtgtcggg cagcccgacc gcgctcagcg
    19801 gcacggacgg gaaggtttcc ggtgcgccgc cgagctggtc ctgctcgtag aacttggtgc
    19861 ggatcacgct gtgcgggcac acgaaagtgc agttgccgca ctggatgcag gtgtcggggt
    19921 cccagaccgc gacgagctcg gagatgttgc gcttttcgta ggccgcggtg ccgctgggat
    19981 acgtgccgtc gaccggcagc gcgctgaccg gcagcgcgtc gccacgtccg gcgatcatct
    20041 cggcggtgac ggtgcgcacg aactccggcg cggtgacagg cacgacaggc cccggttcgt
    20101 aggtggcggt gacccggtcg ggtacctcga cccggcgcag gccgtcgagc gcgcggtcca
    20161 ccgccgccag ctcacggttc aacacgtctt cgccgtgctt gccgtaggcc ttcgtgaccg
    20221 cctcgttgat ccgcacgatc gccttgtccc ggggcagcac ctcggagatt gcaaaaaagc
    20281 acgtctgcag cacgacgttg atccgcccgg cgagtccgac gtcgctggcg atccgcccgg
    20341 cgtccacggc gtacacccgg acgcccttgt cgatgatctg ctgctgcacg gggcggggca
    20401 gcgagtccca caccttgtcg gggggacgcc gcgagttcag cagcagcgcc gcaccgggcg
    20461 ccgcacagcc caccacgtcg gccgcggcga ggaaccgaga gtggtggcag ccgacgaagt
    20521 cggcctgcgc cagcagatac ggcgccttga tgggctcggg gccgaaccgc agatgcgaaa
    20581 ccgtctgtga gccggacttt ttcgagtcat agacgaaata gccttgcgca tagaggtttt
    20641 cctcggcgcc gaggatcttg atcgtgttct tgttcgcacc gacggtgccg tccgaaccca
    20701 cgccgaagaa caccgcccgc accgtgtcgg gcgattcgat gtcgaacgcc gggtcgtagc
    20761 cgatgctgga cccggagacg tcgtcgtcga tacccacggt gaaccgcggc cggggctgtt
    20821 cgcgggccag ctcgtcgaac acgccggcga ccatcgccgg agtgaactcc ttcgacgaca
    20881 ggccgtagcg tccgccgcac acccgcggca tggccgagcg ctcgccgcgg caataggcgt
    20941 cggtcagcgc ggcgacgacg tcgaggaaca gcggctcgcc gatcgagccc ggttccttgg
    21001 tgcggtccag caccgcgacg gtgcgcgccg tggacggcag cgcggccagc agcgcctcgg
    21061 tgggaaaagg ccggtacagc cgcagctgca ccaccccgac acgttcgccg cgcgcggtga
    21121 gcgcggccac ggtctgggcg gcggtctgcg cgccggagcc catcaggacc agcacgcgct
    21181 cggcctcggg gtggccggtg tagtcgacga tgtgcatccg gcggccggtg cgctcggcga
    21241 gggccgccat catctcctcg accaccgccg gcacgcgcgc gtagaacggg ttcaccgtct
    21301 cgcgggcctg gaagtagatg tccgggttct gcgcggtgcc gcggatgaac ggccgctccg
    21361 gagacagccc gcgcgcacgg tgctctcgaa ccaactcttc tggaatcagc gcccgcaggt
    21421 cctcatccgc gagcatctcg atcgtgttga gctcgtggga ggtgcggaag ccgtcgaaga
    21481 agtgcaggaa cggcacccgg gtgcgcagcg tgaccgcctg cgccaccagc gccaggtcgt
    21541 gggcctcctg caccgaggcc gacgacagca acgcgaaccc ggtctgccgc accgccatca
    21601 cgtcctggtg gtcgccgaag atcgaaagac cctgcgctgc aatggctctc gcggccacat
    21661 ggatcactgc cgaggtgagc tcacccgcga tcttgtacat gttcgggatc atcagcagca
    21721 ggccctgcga cgacgtgaat gtcgtcgtca gcgctccggc ctgcagcgag ccgtgcagcg
    21781 cgcccgccgc accgccctcg ctttgcatct ccaccaccgt cggcacggtg ccccagacgt
    21841 tgggccgctg acggctcgac cattcgtcgg ccagctcggc catcggcgag gacggcgtga
    21901 tcgggtagat gcagcacact tcgttgagcc ggtaggcgac cgatacggcc gcctcgttgc
    21961 cgtcgatcgt ggttcgcgtc atctgggctc cgggatcatc tcgatggcgt gtaccgggca
    22021 ctggtcaaag catgtgccgc agcccgtgca tttgtcgtag ttgaattcgt aaccctggtg
    22081 gcccattggc accttgttga tcgcgtcctc ggggcaggag ccgaagcacc cgtcacactc
    22141 gaaacagttg ccgcacgaca ggcatcggcc cgcttcgtac gccgcggcct gtgccgttag
    22201 gccacccacg acttcctcga agccggcaac ggcctcgtca gggggcaatt ccggctgcgc
    22261 gcggcgggcc gcgtcaccga agtaccacag atgcaacttg tcgaacgtcg cgaccggatg
    22321 cttggggggc cgctcgtccg aggtgtcgcg caaccacgca tcgatgtgat gggccgcctt
    22381 cttgccgtga ccgacgccga cggtgacggt gcgctcgcag ggcaccatgt ccccgccggc
    22441 gaacacgccg gggtagcccg tcatcagcgt cggcgacacc agcacgttgc cgtcgtcgtc
    22501 gaaatcgacc ccgggcaggg tgcgcatgaa cgccgactcg gtctcctggc ccaacgccat
    22561 gatcaccgtg tcggcggcca gcgtctcgaa ccgcccggtc gggcgaggct tgccgttgtc
    22621 gtcgagttcc atgacctcga cctgcatctc gtgccggtca aaggcggtga tcgtgcgcag
    22681 ccagtggatc tgcacgcctt cgcgctcggc gtcctgggcc tcctcctc
//
LOCUS       DASWBF010000166         4549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_601773,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000166 DASWBF010000000
VERSION     DASWBF010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601773"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /gene="sulP"
                     /locus_tag="VF874_05650"
     CDS             complement(<1..1532)
                     /gene="sulP"
                     /locus_tag="VF874_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEX9175588.1"
                     /translation="MKLSVAAPGLTQFRYYKRTWLRGDVLAGLTVAAYLIPQVMAYGS
                     LAGLPPVAGLWAALGPLVLYAVFGSSRQLSVGPESTTALMTAVVLTPMVVGDAGRYTA
                     LAAAVAILVGAACFLAGIARLGFLANLLSRPVLVGYMTGIAIVMVVSQLEKLTGIPVG
                     GEEFIDQIRSFTAGVAQAHWPTIALSAAVLVALLAMDRWLPRTPGPLIAVLAATAAVW
                     LFSLDSNGIAVVGQIPSGFPKPGMPDISMHDFASLVIPACGIAIVAFSDNILTARTFA
                     ARRGEDLDANAELRALGLCNAAAGVAQGFPVSSSGSRTALGDAAGGRTQLFSLIMLVM
                     VVAVMLFGRDVLASFPKAALGALIVYAATRLVDVQAFRRLARFRRSEFFLACLTTASV
                     LVFGLLYGVLVAVGLSILDLLRRVARAHDSVLGFVPGLAGMHDIDDYPEAEPVPGLLV
                     YRYDAPLCFANAEDFRKRALRAVHMNPAPVEWFVLNAEANVEVDLTALDALDDLRAEL
                     TSR"
     gene            complement(1693..2088)
                     /locus_tag="VF874_05655"
     CDS             complement(1693..2088)
                     /locus_tag="VF874_05655"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEX9175589.1"
                     /translation="MCGANETKPVYKVLLFLKRRPGMSMEAFRDYYENTHSKLGEKYS
                     RGLVSYVRRYLDPVGSEELPFDVITELWLQDRATAEAIAAHTAKNQPPPEVLEDEKLL
                     FDRSKLRVATVVEFDSELDGVDTDPATAM"
     gene            complement(2153..2839)
                     /locus_tag="VF874_05660"
     CDS             complement(2153..2839)
                     /locus_tag="VF874_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9175590.1"
                     /translation="MIDAGAIQAVATLGEDPRRRLYTFARAARRPVSRDEAAEAVGIS
                     RKLAAFHLDKLVDAGLLRPSGAAGVRRVGRPRKVYEPADAVVQVSIPARRHEVLAEIL
                     AQAVVDEGAHGSAKAAASRAAYERGLTAGVDVRERVRPGRIDAERALALTQEVLSQYG
                     FEPDGCEVGCIRLRNCPFHPLAQKMPELVCQLNLAFMQGLIEGLQASKVDAVLAPTAG
                     ECCVELRGRG"
     gene            2918..3430
                     /locus_tag="VF874_05665"
     CDS             2918..3430
                     /locus_tag="VF874_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX9175591.1"
                     /translation="MSSQLEHAKQPVSRVLAGPYGHPFHPILVTVPIGAWLCSLVFDI
                     ASQMVDEPAFLTQASEWLIGIGVLGAAAAAAVGFLDLIAIPSGTRAFRTGLLHMSLNL
                     AVTVAYIVNFVWRQGDYTSGAAVGIGPLILSAASFATLGASGYLGGMLAYHYGVRVAS
                     EVTQAEGFRS"
     gene            3459..3872
                     /locus_tag="VF874_05670"
     CDS             3459..3872
                     /locus_tag="VF874_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175592.1"
                     /translation="MGIAALVAWIATAGGGAFMLAKWVGGGGHRQPSNTKLPPAVVLG
                     HFALAATGLVLWIVYVAADNRTIGWIAIVALVIVAVLGFVMLARWVPTYRARQTVGGQ
                     PTADAPPEHSFPVAVVVAHGALAVVTVVLALLAIR"
     gene            complement(3975..4257)
                     /locus_tag="VF874_05675"
                     /pseudo
     CDS             complement(3975..4257)
                     /locus_tag="VF874_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942498.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4367..>4549
                     /locus_tag="VF874_05680"
     CDS             4367..>4549
                     /locus_tag="VF874_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX9175593.1"
                     /translation="MTDEDVARRYELVRPHLDEKARRLVLGAEAVALGRGGTKRVAEA
                     TGAHPDTVARGRREVQS"
BASE COUNT          791 a         1540 c         1463 g          755 t
ORIGIN      
        1 cgactggtca attcggccct gaggtcatcg agcgcgtcca gcgcggtgag gtcgacctcg
       61 acgttggctt ctgcgttgag cacgaaccac tccaccggcg ccgggttcat gtggaccgct
      121 cgcagtgcgc gcttgcggaa gtcctcggcg ttggcgaagc acagcggcgc gtcgtagcga
      181 tacaccagca gccctgggac cggttcggct tcggggtagt cgtcgatgtc gtgcatgccg
      241 gccaggccag gcacaaatcc gagcacgctg tcgtgagcgc gtgcgacacg gcgcaggaga
      301 tcgagtatcg acagacccac cgcaaccagc accccgtaga ggagaccgaa aaccaagacc
      361 gacgcggtcg tcaagcacgc gaggaagaac tcgctgcgac gaaatcgcgc cagccgccga
      421 aatgcctgca cgtcgacgag gcgcgtggcc gcgtagacga tcagtgcgcc gagcgcggcc
      481 ttggggaacg acgccagcac atcgcggccg aacagcatca ccgccaccac catgaccagc
      541 atgatcagcg agaacagctg cgtacgaccg ccggccgcgt caccgagtgc ggtgcgactg
      601 ccgctggagc tgaccgggaa accctgtgcc acaccggccg ccgcgttgca caacccgagg
      661 gcacgcagct cggcgttggc gtccaagtcc tcgcctctgc gggccgcgaa cgtgcgcgcg
      721 gtcaagatgt tgtcggagaa cgcgacgatc gcgatgccgc acgcggggat caccagcgat
      781 gcgaaatcgt gcatcgagat gtcgggcata cctggcttcg ggaacccact cggtatctgt
      841 ccgaccacgg caatcccgtt ggagtccaac gagaacagcc atacggccgc ggtcgccgcg
      901 aggaccgcga tcagcgggcc cggtgtgcgc ggtaaccacc ggtccatcgc cagcagcgcc
      961 accagtaccg cagccgacag cgcgatcgtc ggccaatgag cttgcgccac accggccgtg
     1021 aacgagcgta tctggtcgat gaattcctcg ccgccgaccg ggatgccggt gagcttttcc
     1081 agctggctga caaccatcac gatcgcgatg cccgtcatgt agcccaccag caccgggcgc
     1141 gacaacagat tggccagaaa cccaagccgc gcgatgccgg ccagaaagca ggctgcgccg
     1201 acgaggatgg cgacggcggc cgccagggcg gtgtagcgcc ccgcgtcacc cacgaccatc
     1261 ggggtcaaca cgaccgccgt catcagtgcg gtggtggact ccgggccgac ggacaactgc
     1321 cgcgacgagc cgaacaccgc atagaggacc agcggtccca aagctgccca cagccccgcg
     1381 accggcggca gacccgccag gctgccgtag gccatcacct gcgggatgag gtaagcggcc
     1441 acagtgaggc cggcgagcac gtcgccgcgc agccatgtcc gtttgtagta ccggaactga
     1501 gtcagccccg gggcggcgac ggaaagtttc atggtggcct cattgtgcgg cgacctgccc
     1561 cgtcaggtgc gtgattcaca atggcggcga ttctgcgccg aatggcacga gagcaaccgt
     1621 cggcagatcg atgatcgcat ggctcgtagc cagttctggg cagtcgaagg gccgccgatt
     1681 ggccgatgag tgtcacatag ccgtggccgg atcggtgtcc acgccgtcga gttcagagtc
     1741 gaattccact acggtcgcca ctcgtagctt cgagcggtcg aataggagtt tctcgtcctc
     1801 gagcacttcg ggaggcggct gattcttggc ggtgtgcgcc gcgatcgcct cggcggtggc
     1861 ccggtcttgc agccacagtt cggtgatgac gtcgaacggc aattcctcgc tgccgaccgg
     1921 atcgaggtag cgccgaacgt agctcaccaa cccgcgcgag tacttctcgc cgagcttcga
     1981 gtgggtgttc tcgtaatagt cgcggaaggc ctccatcgac atgccgggcc ggcgcttgag
     2041 gaacagcagc accttgtaca ccggcttcgt ctcgttagcg ccgcacatca ggaatcaccc
     2101 acgatctgac cggtcatgta tcccgccttc tcggacgctc catatcgtct ggtcaccccc
     2161 tgccgcgaag ttccacgcag cactcgccgg ccgtcggcgc gagcacagcg tcgaccttcg
     2221 acgcctgcaa tccctcaatc aagccttgca tgaaagctaa gttcagctga cataccaatt
     2281 ccggcatctt ctgcgcgagc ggatgaaatg ggcagttgcg caggcgaata cagcctactt
     2341 cgcagccgtc gggctcaaat ccgtactgcg acaagacttc ctgcgtgagg gccagtgccc
     2401 gctccgcgtc gatgcggccc ggccgcaccc gttcccgcac gtccacgccg gcggtaagtc
     2461 ctcgttcgta cgccgcccgt gaggcggcgg ctttggccga cccgtgcgcg ccttcgtcga
     2521 ccaccgcctg agccaagatc tccgcaagca cctcgtgccg ccgcgccggg atgctcacct
     2581 ggaccacggc gtccgcgggt tcgtacacct tccgcggacg gcccacccgc cgcacgccag
     2641 cggctccaga cggccgcagc aagcccgcat cgaccagttt gtccaggtgg aacgccgcca
     2701 gcttgcgcga gatgccaaca gcttcagcgg cttcgtcgcg actcaccggc cgccgagccg
     2761 cccgcgcaaa cgtgtacagg cgccgccggg gatcctcgcc caacgtcgca acggcctgga
     2821 tagcacccgc gtcgatcacc ccgcacccct cctcttgacg ctttcgcgaa taagactaac
     2881 attaattgtc taaatccctg cgctgggaga ggcggccgtg agcagtcaac tcgaacacgc
     2941 gaagcaaccc gtcagccgcg tactggccgg accgtacgga cacccgttcc atccgatcct
     3001 cgtcaccgtg ccgataggcg cctggttgtg cagcctcgtc ttcgacatcg cctcacagat
     3061 ggtcgacgag ccggcgtttc tcacgcaggc ctccgaatgg ctgatcggca tcggcgtgct
     3121 gggcgcggcg gcggcggcgg cggtcggctt cctcgatctg atcgcgatcc cgtcgggcac
     3181 ccgcgccttt cgcaccgggc ttctgcacat gtcactgaac ctcgccgtca ccgtcgccta
     3241 catcgtcaac ttcgtatggc gacaaggcga ttacacctca ggcgcggccg tcggcatcgg
     3301 cccactgatc ctgtcggcgg ccagcttcgc cacgctgggc gcatcggggt acctcggcgg
     3361 catgctcgcc taccactacg gagtgcgcgt cgcctccgag gtcacccaag ccgaaggatt
     3421 ccgttcctga tccgtcgcga aagcaggaag gacacatcat gggtatcgca gcactcgtcg
     3481 cctggatcgc caccgcagga ggcggcgcct tcatgctcgc caagtgggtg ggcggcggcg
     3541 gacaccgcca gcccagcaac accaagctcc cacccgcagt ggtactcggc cacttcgctt
     3601 tggccgcaac cggtctggtg ctgtggatcg tctacgtggc ggccgacaac cgcacgatcg
     3661 gctggatcgc gatagtcgcg ctcgtgatcg tcgccgtcct tggcttcgtc atgctcgcgc
     3721 gctgggtccc cacctaccgt gcacgacaga cagtgggcgg ccagccgacg gccgacgctc
     3781 cgccggaaca ctcgttcccg gtggcggtcg tcgtcgcgca cggcgcgctg gcagtggtca
     3841 ccgtcgtcct tgcgctgctt gcaatccggt aagggcggcg gctcgtcgtc agcatcggag
     3901 gatgcaggcg ttccgaaata ggcttcttcc caagcttttc ggcgcgcttg tcgcagcttc
     3961 tggttgcggg cgggttcggc ggccaggtat ttcgcgcggt tctcggcgcg agtggtcttg
     4021 cgccgcggca tcatcaccca cggatcgccc tggcggttgg cgcacttggg gcccggcggc
     4081 ggcaactctc cggtgggggc acaacgtcgg gaatataggg gcgcttccgg gcagggcgac
     4141 gtaggtctgt cccgacggca gcgtccagat caccgtgccg tcgggcagct gcttttcctg
     4201 ccacccccag aacgtcttga gcagatgatg aaacctacaa aggcacttga gattattgac
     4261 aagtcgctaa tatgtcctct cggcaatgcc gctcccttag ggctcgctga tcaattagct
     4321 gggtttcgtg gtgcggcgga ggcttggttt gtgctatgtg gcttgtgtga ctgatgagga
     4381 tgtagcgcgc aggtacgagc tggtgcggcc gcacctggat gagaaggcgc ggcggctggt
     4441 gctgggggcc gaggctgtgg ccctgggccg aggcgggacc aagcgggtcg ccgaggcgac
     4501 cggggcgcac ccggacacgg tggcccgtgg ccggcgggaa gtccagtcg
//
LOCUS       DASWBF010000167         7238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1130649,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000167 DASWBF010000000
VERSION     DASWBF010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130649"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VF874_05685"
     CDS             complement(<1..641)
                     /locus_tag="VF874_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9175594.1"
                     /translation="MTTNARLRALVELADSGSVRGAAERLVVTESAISSAISSLSAEL
                     GVALVDRYGRGVRLTPAGLRYTEYARRILGLHDEALRAARSEADPQKGSIRLAAVTTA
                     GELLIPALLASFRAEHPGVTCELEVRPRSSVWAMLSRHEADVVVAGRPPADLAQAQVQ
                     ATSPNTLVVVGPPTAAHGFEPAGATWLLREAGSGTRATTAMLLEELEISPPRMT"
     gene            complement(638..1645)
                     /gene="cbbX"
                     /locus_tag="VF874_05690"
     CDS             complement(638..1645)
                     /gene="cbbX"
                     /locus_tag="VF874_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HEX9175595.1"
                     /translation="MPSYSAGETTRQSFGYTPRSESGPQLAPEPAETRPPPLPADAVV
                     DLADARKKAGVDEVMTKLDCELVGLTPVKTRIAEIAALLLVDRMRARYGITAPQPTLH
                     MCFTGNPGTGKTTVALRMADLLHRLGYLRRPHLTTVTRDDLVGEYVGHTAPKTKEVIK
                     RAMGGVLFIDEAYYLYKVENERDYGSEAIEILLQVMENHRDDLVVILAGYADKMDRFF
                     SANPGMQSRIAHHITFPDYTIDELEQIAVLMSRELGYGLSDEAQVVLRQYLHRRIHQP
                     WFANARSVRNAVERARLRQARRLVHGADPKVNRHALTTIEAPDLLASRVFEHAVSEEA
                     V"
     gene            complement(1647..2066)
                     /locus_tag="VF874_05695"
     CDS             complement(1647..2066)
                     /locus_tag="VF874_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HEX9175596.1"
                     /translation="MRITQGTFSYLPDFTDEEITAQINYALDNGWPLSVEFTDDPHPR
                     NVYWEMWGLPMFDLTDAAGILLEVNACRAAYPDSYVRLNAYDARLGRQTTAFSFIVQR
                     PANEPGFRLDREEGSDRRIGYTTFAYATRDPAGSRYR"
     gene            complement(2063..3469)
                     /locus_tag="VF874_05700"
     CDS             complement(2063..3469)
                     /locus_tag="VF874_05700"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003252.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HEX9175597.1"
                     /translation="MGYWQPDYVPKDTDVLCAFRITPQPGVAPEEAAAAVAGESSTAT
                     WTVVWTDRLTAHEQYQAKCFRIEPVPNAPGQWIGWIAYDLNLFEEGSIANLTSSIIGN
                     VFGFKPLKALRLEDMRIPIAYVKTFQGPAHGVVMEREMLNKFGRPLLGATTKPKLGLS
                     ARNYGRVVYEALRGGLDFTKDDENINSQPFMRWRDRYLFCMEAVNRAQAATGEIKGHY
                     LNVTAATMEDMYERAEFAKELGSVVVMIDLTIGYTAIQSMAKWARANSVILHLHRAGH
                     ATFTRQKTHGVSFRVLAKWMRLAGVDHIHAGTVVGKLEGDPRTTAGYYDTLRLDNVTA
                     DAAKGLYFDQQWASMPAVMPVASGGIHAGQMHQLVHYLGEDVVLQFGGGTIGHPMGIA
                     AGAEANRFALEAMIKARNEGRDYYREGPDILKKAASRNRALDTALATWVTSRSTTNRP
                     THPMWSRHRQSVENRRTP"
     gene            3621..4874
                     /locus_tag="VF874_05705"
     CDS             3621..4874
                     /locus_tag="VF874_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX9175598.1"
                     /translation="MAVLLAYGSPALGHFLPLSALLSALTARGHQVHLRTLSGGVTAG
                     REFGFAADRVDPRIEAIESRDWTARNAFDVLAMTVDVLCRRAVLEVDDVRRAIERVQP
                     DALIVDANCWGAISVADAGDIPWSVFCPFTPYLRSRGVPPFGPGLRPLPGILGRVRDA
                     QVRPVVTRLFDRRMLPRVNAIRTELGAQTVGSVDELMRRAPLLLAVGGEPFEYPHPDW
                     GDSVHLIGACTPPQAAAEVPDWLADIDRPIVLVSTSSIRQADAVLGVTALEALADEPV
                     HVVATFPAGVPDGLPVPANATVRQFVPHDAVLERAVCAITHGGMGTTVRALDRTVPVC
                     VVPFARDQAEVARRVEVARCGTRLPAKRLTAQRLRAKVRDAMTMTDGAARVAAGFAAT
                     GGTARGAKLIEQHLLAGRGMTSPLP"
     gene            complement(4792..5208)
                     /locus_tag="VF874_05710"
     CDS             complement(4792..5208)
                     /locus_tag="VF874_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEX9175599.1"
                     /translation="MYVAVVILAVSAHFAFLIYVASGGFLALRWRRTIWLHVPAVLWG
                     IGVVVWHLACPLTWLEGWARAHAGMGSLPGSGFIGHYVAGVLYPSNDTGIAQALAFSA
                     VLVSWVAYAVTATGTSYRGQPADVARSASHRGQSRP"
     gene            complement(5250..5849)
                     /locus_tag="VF874_05715"
     CDS             complement(5250..5849)
                     /locus_tag="VF874_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX9175600.1"
                     /translation="MRNTSQRMALVGGAAAIAAAAALPLVSGTASAEPMTGLVGPACA
                     SYAQQVPTGPGSVAGMAQDPVAVAASNNPLLTTLTAALSGKLNPNVNLVDTLNGGQFT
                     VFAPTDDAFAKLPPATIDALKTDSAGLTSILTYHVVPGQISPNNIAGTHKTVQGQSVT
                     VTGMGDSIKVNNASVVCGGVQTANATVYLIDSVLMPPTQ"
     gene            5929..6600
                     /locus_tag="VF874_05720"
     CDS             5929..6600
                     /locus_tag="VF874_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9175601.1"
                     /translation="MGVTTDHVVVSDLGPTEWGARTQGVGPWPGPPPDDPRYDPQLLR
                     DGDTRNVVDAYRYWSREAIIADIDKRRHPLHIAIENFGHDANIGGVVRTANAFAVDTV
                     HIVGRRRWNRRGAMVTDRYQRLRHHETTAELLAFAEEAGLAVVAVDNVPGAVRLEETT
                     LPRDCLLVFGQEGPGITDEARAGAALTVSIAQFGSTRSINASVAAGIAMHTWIREHSD
                     LSRAW"
     gene            6616..>7238
                     /locus_tag="VF874_05725"
     CDS             6616..>7238
                     /locus_tag="VF874_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HEX9175602.1"
                     /translation="MEQQWANRAASAEAAITKRHLSRVWSMPGTQLGVVAWPSTRQDR
                     LFASWNYWWQAHLLDCLVDAQVRDPQPQRRKRIAAQIRGHHLRNIGRWTNSYYDDMAW
                     MALALERSGRLAGVERQRALRTFTKQFTRAWMPEFGGGIPWRKQDRFFNAPANGPAGI
                     FLARNGELRQAQMMADWIDETLIDPETHLVFDGIKEGSLDRAMFTYC"
BASE COUNT         1152 a         2352 c         2432 g         1302 t
ORIGIN      
        1 gtcatccgcg ggggcgaaat ctcgagctcc tccaagagca ttgcggtggt ggcacgggtt
       61 cccgacccgg cttcgcggag cagccacgtc gcacccgccg gctcgaatcc gtgcgcggcg
      121 gtcgggggcc cgaccaccac cagagtgttc gggctggtcg cctgtacctg cgcctgcgcc
      181 aagtccgccg gaggacggcc cgcgaccacc acgtcggcct cgtgccggga cagcatcgcc
      241 cacaccgaac ttcgaggtct tacttcgagc tcgcaggtga ccccgggatg ctccgcccga
      301 aacgacgcca gcagagcggg aatcagcaat tcgccggccg tggtgacggc cgccagccgg
      361 atcgagccct tctgcgggtc agcctcgctt cgggccgctc ggagcgcttc gtcatgtagc
      421 ccgaggatcc ggcgcgcgta ctcggtatag cgaagtccgg caggagtgag acggactccg
      481 cggccgtagc ggtccacaag ggccacaccg agttccgcgc tgagcgagct gatcgccgac
      541 gaaatcgcgg actcggtcac cacgagtcgc tccgcggcgc cgcgcaccga cccgctgtcg
      601 gccagctcga cgagtgctcg cagccgcgcg ttcgtggtca caccgcctcc tccgagaccg
      661 cgtgctcgaa cacccggctg gccagtagat ccggtgcttc gatcgtcgtg agtgcgtgac
      721 ggttcacttt gggatcggcg ccgtggacca agcggcgggc ctgccgtagg cgtgcgcgtt
      781 ccacggcgtt gcgcacgctg cgcgcgttcg cgaaccacgg ctggtggatt cgacgatgca
      841 gatattgccg cagcactact tgtgcttcgt ccgacaaccc gtagcccagt tcgcggctca
      901 tcagcaccgc gatctgttcc agttcgtcga tcgtgtagtc ggggaacgtg atgtggtggg
      961 cgatccggct ctgcatgccg gggttcgccg agaagaaccg gtccatcttg tcggcgtagc
     1021 ccgccaggat cacgacgagg tcgtcgcggt ggttttccat tacctgcaac aggatttcga
     1081 tagcctcgga gccgtagtcg cgttcgttct cgaccttgta caggtagtac gcctcgtcga
     1141 tgaacaacac gccgcccatg gcgcgcttga tgacttcctt ggtcttgggc gcggtatgcc
     1201 cgacgtattc gccgacgaga tcgtcgcggg tgacagtagt caggtgcggc cggcgcaagt
     1261 accccagccg atgcagcaga tcggccatcc gcagtgccac ggtcgtcttg cccgtgcccg
     1321 ggttccccgt gaagcacatg tgcagtgtcg gttgcggcgc cgtgatgccg taccgggccc
     1381 gcatccgatc aacgagcagc agcgctgcga tctcggcgat tcgggttttc accggtgtca
     1441 gcccaacgag ctcacagtcc agcttcgtca tcacttcgtc gacaccggcc ttcttgcggg
     1501 catcggcgag atcgacgacc gcgtccgcgg gaagtggcgg tggccgtgtc tcggcgggct
     1561 cgggcgcgag ctgtgggccg gactcggatc gtggcgtgta tccgaaggat tggcgcgtgg
     1621 tttcgcccgc tgagtatgac ggcatctcag cgatagcggc tgcccgccgg gtcgcgggtt
     1681 gcgtaggcaa acgtcgtgta accgatgcgc cggtcggagc cctcttcgcg gtcgagccgg
     1741 aacccgggtt cgttggcggg acgctgcacg atgaacgaga acgcggttgt ctgacggcct
     1801 aaccgggcgt cgtaggcgtt gagccgcaca taggagtcgg gatacgcggc gcggcaggcg
     1861 ttgacctcca gcagaatccc cgcggcgtct gtgagatcga acatcggcag tccccacatc
     1921 tcccagtaga cgttgcgggg gtgcgggtcg tcggtgaact caaccgacag cggccagccg
     1981 ttgtcgagcg cgtagttgat ctgggccgtg atctcctcgt cggtgaagtc cggcaggtac
     2041 gagaacgtgc cctgggtgat tctcatggcg tcctcctgtt ctcaactgac tgtcggtgtc
     2101 gcgaccacat cgggtgtgtc ggtcgattcg tagttgaacg tgatgtcacc cacgtcgcca
     2161 gcgcggtgtc cagtgcgcgg ttgcgggatg ctgccttctt caggatgtcg ggcccttcgc
     2221 ggtaatagtc gcggccctcg ttgcgtgcct tgatcatcgc ctccagcgcg aaccggttcg
     2281 cctcggcccc ggccgcgatt cccatggggt gcccgatggt gccaccgccg aattgcagca
     2341 cgacatcctc gccgagatag tgcaccaatt gatgcatttg gccggcgtgg atacctcccg
     2401 acgcgaccgg catgaccgca ggcatggatg cccactgctg gtcgaagtag agacctttcg
     2461 ccgcgtcggc ggtgacgttg tccagccgaa gcgtgtcgta atagcctgcg gtggtgcgcg
     2521 ggtcgccttc cagcttgccc actaccgttc cggcgtggat gtggtccacg ccggccaacc
     2581 tcatccactt ggcgagcacc ctgaagctga cgccatgagt cttctggcga gtgaaggtgg
     2641 cgtggccggc gcggtgcaag tgcaagatca cgctgttggc gcgggcccat ttcgccatcg
     2701 actgaattgc ggtgtagccg atggtcagat cgatcatcac gaccaccgat ccgagttctt
     2761 tggcgaactc ggcacgctcg tacatgtctt ccatcgtggc ggcagtgacg ttgaggtaat
     2821 ggcccttgat ctcaccggta gccgcctgcg cccggttgac ggcctccatg cagaagagat
     2881 agcggtcgcg ccaccgcatg aagggttgcg agttgatgtt ctcgtcatcc ttggtgaagt
     2941 ccaggccgcc gcgcagggct tcatagacga cgcggccgta gttgcgcgcg gagagaccga
     3001 gtttcggctt cgtggtagca cccagcaggg gccgcccgaa cttgttgagc atctcgcgtt
     3061 ccatcaccac gccgtgcgcc ggaccctgga atgtcttgac gtaggcgatc ggaatccgca
     3121 tgtcctccaa tcgcaatgcc ttcagcggtt tgaagccgaa gacgttaccg atgatcgagc
     3181 tggtgaggtt cgcgatggag ccctcctcga aaaggttgag gtcatatgcg atccagccga
     3241 tccattggcc gggagcgttg ggtaccggct cgatccggaa acacttggcc tggtactgct
     3301 cgtgggcggt gagcctgtcg gtccagacaa cggtccatgt tgccgtgctc gactcacccg
     3361 cgaccgccgc tgcggcttcc tccggcgcga cgccgggttg cggggtgatg cggaaagcgc
     3421 acaggacgtc ggtgtccttc ggcacatagt cgggttgcca gtagcccatc tcggcatagg
     3481 cgacgacccc cgcgcttcag cgatctgtca tgacaacatc gtggcagcgt aatctgaaca
     3541 tgtctattga ggattcgcca tggaaacttt agtattcact tcattgaagt ccgcgagacc
     3601 gtccgtgccg aataactgac gtggcggtcc tacttgccta cggctccccg gcgctcgggc
     3661 actttctgcc tctcagcgca ctgctgtcgg cgttgaccgc tcgtggtcac caggtgcacc
     3721 tgcgtacgct gtccggcggc gtcaccgccg gccgagaatt cggcttcgcc gctgaccgcg
     3781 tcgatccgcg gatcgaggcg atcgaaagtc gggactggac cgctcgcaac gccttcgacg
     3841 tgctcgcgat gaccgtcgac gtgctgtgtc ggcgagcggt gctcgaagtc gacgacgtgc
     3901 gccgcgccat cgagcgcgtg cagccggacg cactgatcgt cgacgcgaac tgctggggtg
     3961 ccatctcggt ggccgacgcc ggtgacattc cgtggtcggt gttctgcccg ttcaccccgt
     4021 acttgcgctc ccgcggggtg ccgccattcg ggccggggct gcgtccgctg ccaggaattc
     4081 tgggccgggt gcgcgatgct caagtgcggc cggtcgtcac gcggctgttc gaccgtcgaa
     4141 tgctgccgcg cgtcaacgcg attcgcaccg agctgggtgc gcagaccgtc ggatccgtcg
     4201 acgagctgat gcgtcgggcg ccgctgctgt tagcggtcgg cggggagccg ttcgagtatc
     4261 cgcatcccga ctggggcgac agtgtgcacc tgatcggcgc gtgcacgccg ccgcaagcag
     4321 cggcggaggt acccgactgg ctggcggaca tcgaccggcc gatcgtgttg gtcagtacct
     4381 catcgatcag gcaggccgac gcggtgctgg gtgtcacggc gctcgaggcg cttgccgacg
     4441 agccggttca tgtggtcgcc acgttcccgg ccggggttcc tgacgggctg cctgttcccg
     4501 cgaatgcgac tgtgcgccaa ttcgttccac atgacgctgt actcgaacgc gcggtgtgcg
     4561 ccatcactca tggcggaatg ggaaccacgg tcagagcact cgaccgcact gtgcccgtct
     4621 gcgtcgtgcc gttcgcccgc gaccaggcag aggtggctcg ccgcgtcgaa gtggcgcggt
     4681 gcggcacccg gctgccggcg aaaaggctga ccgcacaacg cttgcgcgca aaggtgcgcg
     4741 acgcgatgac gatgacagac ggtgccgccc gggtggcggc tggcttcgcc gctacgggcg
     4801 ggactgcccg cggtgcgaag ctgatcgagc aacatctgct ggctggccgc ggtatgacgt
     4861 ccccgttgcc gtgaccgcgt acgcgaccca cgacaccagc accgccgaaa aggcaagtgc
     4921 ctgagcgatt ccggtgtcgt ttgacgggta cagcacaccc gcgacgtagt ggccgatgaa
     4981 ccccgagccc ggcagcgagc ccatcccggc gtgcgctcgc gcccagccct ccagccatgt
     5041 caacgggcag gccaggtgcc acaccacaac cccgattccc cacagcaccg ccggcacatg
     5101 cagccagatg gtgcgtcgcc atcgcagcgc gaggaacccg ccgctcgcca catagatcag
     5161 gaacgcgaaa tgcgcactga ccgcgaggat cacgactgcg acgtacacat catcactgtc
     5221 ccacggcggc gggctcccgc gctatgaggt cactgcgtcg gcggcatcaa caccgagtcg
     5281 atgaggtaca cggtcgcatt ggccgtctgc acgccgccgc agaccaccga cgcgttgttg
     5341 accttgatgc tgtcacccat gccggtcacc gtgaccgact ggccctgcac cgtcttgtgg
     5401 gtgcccgcga tgttgttcgg gctgatctgc ccgggcacca cgtggtaggt gaggatgctg
     5461 gtcaatcccg ccgagtcggt cttgagcgcg tcgatcgtcg caggcggcag cttcgcgaac
     5521 gcatcgtcgg tgggtgcgaa caccgtgaac tgcccgccgt tgagcgtgtc gacgaggttg
     5581 acgttggggt tgagtttccc ggacagtgcg gcggtcaacg tggtcagcag cgggttgttg
     5641 gacgcggcca ctgcaaccgg gtcctgggcc attcccgcca cggatccggg tccggtgggc
     5701 acctgctgcg cgtagctggc gcatgcgggg ccgacgagac cggtcatcgg ctcggcactg
     5761 gcggtgccgc tcaccagcgg caatgctgcg gctgcggcga tcgccgcggc gccgccgacc
     5821 agggccatcc gttgtgaagt gttgcgcata gttgtcgaac ctcctgtgtc gtgcgaccgt
     5881 cgtcgcgtcg ttgctttcac gagtcattcg gaacccgatc cgattcggat gggagtgacc
     5941 acggatcatg tcgtggtgag cgacttgggc cccaccgagt ggggtgcacg aactcaaggc
     6001 gtcgggcctt ggccaggccc gccgcccgat gacccgcgtt acgacccgca gctgctgcgc
     6061 gacggagaca cccgcaacgt cgtcgacgcc taccggtact ggagccgcga ggcgatcatc
     6121 gccgacatag acaagcgccg ccacccgctc catatcgcga tcgagaactt cggtcacgac
     6181 gcgaacatcg gcggtgtggt gcgcaccgcg aacgcgttcg ccgtcgacac cgtgcacatc
     6241 gtcgggcgcc gacggtggaa ccggcgtggc gcgatggtca cggaccgcta tcagcggctg
     6301 cggcaccacg agacgacggc tgagctgctg gcgttcgccg aggaggcagg gttggcggtg
     6361 gtggcggtcg acaacgtgcc cggtgctgtc cgcctggagg agacgacgtt gccgcgcgac
     6421 tgcctgctgg tgttcggcca ggagggtccg ggcatcaccg acgaggcccg cgccggtgcg
     6481 gctctgaccg tgtcgatcgc ccagttcggg tcgacccgca gcatcaacgc gtccgtcgcc
     6541 gccggcatcg cgatgcacac gtggatccgc gaacattccg acctcagccg cgcctggtga
     6601 ggcaggattc gtgtcatgga acagcagtgg gcgaatcgcg ccgccagcgc tgaggccgct
     6661 atcaccaaac gccacctgtc gcgagtctgg tcgatgccgg gaacgcaact cggtgtggtg
     6721 gcgtggccgt cgacgcggca ggatcggttg tttgcgagct ggaactactg gtggcaggcg
     6781 cacctgctgg actgtcttgt cgacgcgcag gtgcgcgacc cgcagccgca gcggcgcaag
     6841 cgcatcgccg cgcagatccg cgggcaccac ctgcgcaaca tcggccggtg gaccaacagc
     6901 tactacgacg acatggcctg gatggcgttg gcactcgagc ggtcgggccg gctcgccggg
     6961 gtcgagcggc agcgtgccct gcgcaccttc accaagcagt tcacccgcgc gtggatgccg
     7021 gagttcggcg gcggcatacc gtggcggaag caggaccggt tcttcaacgc cccggcgaac
     7081 ggcccggccg gaatcttctt ggcgcgcaac ggagaactgc gccaggccca gatgatggcc
     7141 gactggatcg acgagacgct gatcgatccc gaaacccact tggtgttcga cggcatcaag
     7201 gagggctcac tcgaccgagc tatgttcacc tactgcca
//
LOCUS       DASWBF010000168        11224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_998048,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000168 DASWBF010000000
VERSION     DASWBF010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11224
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998048"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF874_05730"
     CDS             complement(<1..321)
                     /locus_tag="VF874_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9175603.1"
                     /translation="MLLPSLGAGADLGDAVRIGSSNDTVSLSRSDLVGAATSVAERVG
                     GAHRVAVLATPTASTVLAVTGCLIAGVPVVPVPADVGHGERRHILTDSGAQAWLGEAP
                     DEAEG"
     gene            394..948
                     /locus_tag="VF874_05735"
     CDS             394..948
                     /locus_tag="VF874_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9175604.1"
                     /translation="MTITARNLDGYGAPAIEWDRVREVLDGQLTQAPGTVPRKREVPP
                     GPDRHTTWLTTVNHDGSPHVMPVGVMRHGGHWYFTSGPATRKSRNLARDPRCVVSVAT
                     LPFDLVIEGTVQRVTEGDELRSVAQVFVDNGWPAEVDGDALTAEYSAPSAGPPPWYVY
                     RVEPSTLFALGTSEPYGATKFDVT"
     gene            1030..1542
                     /locus_tag="VF874_05740"
     CDS             1030..1542
                     /locus_tag="VF874_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175605.1"
                     /translation="MCVLRRKWTALLGAVLFAVFTASPFSASAESPSWNGKYTLVGYA
                     DQKTGNSRAARQPEAPFSGELVFVTKCSGGSCVATVVDGPRPKNPTVPLPERYTWDGS
                     RWANIYDWQWDCFMGDGAPKVWSPARSWTYYVPQPDGTLRGTWMTEIYGGPCEGSVWM
                     PVAAFPLQRQ"
     gene            complement(1536..2705)
                     /locus_tag="VF874_05745"
     CDS             complement(1536..2705)
                     /locus_tag="VF874_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9175606.1"
                     /translation="MTVEHRRPWLTRNVWVLSLVSFLQDAASELLYPLLPIYLTSVLG
                     APPSVVGAVEGAAEGAAALTKMAAGPLGDRFARRPLIATGYGMAALGKVIVAAAAAWP
                     GVLAGRVVDRLGKGLRGAPRDALLVEDIEPAARGRVFGFHRAMDTAGAVVGPLLGLAG
                     YELLDHQIAPVLWLAVIPAAISVLLVFLVRERARTREARSPVFAGVRRLPGRYWRVAA
                     VLVAFSLANFPDALLLLRLNDIGFGVAGVVLAYVGYNAVYALASYPAGMLADKFTPAT
                     VFGVGLAFFAVGYLGLGLTTDTVLAWLLIGVYGVFTACYDGVGKAWISTLLPPELQSS
                     GQGVFQGLSGFAILFAGLWAGLLWGAAGRLPLLVSGGVGAVFAVGLLLFAARPRH"
     gene            complement(2803..3762)
                     /locus_tag="VF874_05750"
     CDS             complement(2803..3762)
                     /locus_tag="VF874_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX9175607.1"
                     /translation="MGLFTTTARPAILAVSRSERLRRTAERWPVARRVVHRFVPGESL
                     DDALNAVAMLRDSDRMVSIDYLGEDVTDADTADATVDAYLELLDALGRRADARYEGVR
                     PLEVSLKLSALGQALPRDGEKIALENAHAVCTAAQRAGVWVTVDAEDHTTTDSTLSIV
                     RELRADFDWLGAVLQAYLRRTLSDCREFAASGARIRLCKGAYDEPASVAYREPDDVDD
                     SYLRCLHVLMSGSSYPMVASHDPAVIAAAGALARETGRGAGDFEYQMLYGIRDAEQRR
                     LADAGDAVRVYVPFGTQWYGYFVRRLAERPANLTFFLRALAGR"
     gene            complement(3765..5393)
                     /gene="pruA"
                     /locus_tag="VF874_05755"
     CDS             complement(3765..5393)
                     /gene="pruA"
                     /locus_tag="VF874_05755"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX9175608.1"
                     /translation="MDAITDVPMPANEPVHDYAPNSPERTRLAAALNSLAAEPIDLPH
                     VIGGGHRMGSGEPIDVVQPHRHAARLGTLTNATHADAFDAMEAALAAKAQWAATPFDE
                     RAAVFLRAADLLAGPWREKLAAATMLGQSKTAYQAEIDAPCELVDFWRFNVAFARQIL
                     AQQPLSSRGVWNRTDYRPLEGFVYAITPFNFTAIAANLPTAPTLMGNTVVWKPSVTQM
                     LSAYLTMQLLEAAGLPPGVINLVGGDGFAVSDVLLSDPRLAGIHFTGSTTTFQHLWRE
                     VGSHVERYESYPRLVGETGGKDFVLAHASAQPDVLRTALIRGAFDYQGQKCSAASRAF
                     IPRSVWQRMGDDFLSATEALAYGDVADLSNFGGALIDSRAFTKNVAAIERAKGAPGVT
                     IAAGGEYDDSEGYFVRPTVLLSDDPGDEAFRTEYFGPILAVHVYPDDGYDEILDVVDR
                     GARYALTGAVIADDRAAVMAAADRLRYAAGNFYVNDKPTGAVVGQQPFGGSRASGTND
                     KAGSALNLLRWTSPRSIKENFVPPTDHNYPYMEV"
     gene            5478..7124
                     /locus_tag="VF874_05760"
     CDS             5478..7124
                     /locus_tag="VF874_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9175609.1"
                     /translation="MESARVGLGQLLLALDRTMVTLVDAPRGLDMPVESVALVDADDI
                     RLGLAVGAGNADAFFLLGVSDVEALRWLDRHAQKVPAAIFVKEPTDTLVAGATAAGAA
                     VVAVEPRARWERLYRLVTHVLEHHSDRDDPRFASGTDLFGLAQSVAERTHGMISIEDA
                     QSHVLAYSASNDEADELRRLSILGRAGPPEHLAWISQWGIFDALRTGRDVVRVAERPE
                     LGLRPRLAVGIHRDTPPDALRASGSSARRRSGFLGTIWLQQGSRPLADDAEDVLRGAA
                     VLAARIMTRLAATPTTHTLRVQELLGVTGEDVDVTAVARDLGIVADGRAALIGFRCAD
                     GPAPAHAIALSASAFRNDAHTATVGDTVYVLFPQTGAASSVTSWVRGTVAALDRELGL
                     DLRAVIATGIDGLASVAAARADVDRVFDSAGRHPGAIRAVTSTDEARTTVLLDGIVAL
                     MDDDLVDPRLWALGDYDAAHDGSLVETLRAYLDCFGDVAAAGDLLHVHPNTVRYRVRR
                     IEKILGTSLGYPDDRLVLALGLRAMPLTERQPGRSARRSR"
     gene            complement(7031..7684)
                     /locus_tag="VF874_05765"
     CDS             complement(7031..7684)
                     /locus_tag="VF874_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175610.1"
                     /translation="MATSTDAASARNTMHALFASTEEQRGGDDLAPGSTATPFSQFFW
                     AALRHLADGNDSDRKTAEAQFADDVEWDMMNNGQIRRGKADVLAWCWSGGFASHKNPV
                     VIQNSATREWGVWEYWNIGTLTADVIDFAKASRWPFPADLGGLIGREYMIPVCFVYRI
                     NDLGRIDLVREYLDTESLMAQFTRPAAHRDRRADRPGCLSVSGIARNPSASTSRSSG"
     gene            complement(7698..8393)
                     /locus_tag="VF874_05770"
     CDS             complement(7698..8393)
                     /locus_tag="VF874_05770"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9175611.1"
                     /translation="MARQRNLVAAASSVRRESGRDAGTRQRLLETAGRVFADKGLDSA
                     TGREICERAGVNTAAINYHFGGFARLYEAVLTEALERVPSRDALSAALDSAADDQAKL
                     RAIIGLAVDVLTGPPARSWTVRLLVREAAAPSAAFERLTSDSERRAKLSLLKRTIGHV
                     MKLPENHPVAEQGCFAVAALCQVMLIGDRNVVKRNYPGLDLSTQGASELADRLTIFAM
                     AGLKALAAQTASE"
     gene            complement(8502..9590)
                     /gene="dapC"
                     /locus_tag="VF874_05775"
     CDS             complement(8502..9590)
                     /gene="dapC"
                     /locus_tag="VF874_05775"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX9175612.1"
                     /translation="MSASLPVFPWDTLADASALSRSHPDGIVDLSVGTPVDPVAPLIR
                     DALAAASASPGYPTTAGTPALRDAAVRALARRYQVSGLESDAVLPVIGTKELIAWLPT
                     LLGLGAGDIVVVPELAYPTYEVGARLAGAQVLLADSLTQLGPQRPALIYLNSPSNPTG
                     RVLGVDHLRKVVGWARERGALVVSDECYLGLGWDEQPLSLLDDAVCGGDHTGLLAAHS
                     LSKSSSLAGYRAGFVAGDPAVVAELLAVRKHAGMMMPTPVQAAMVAALDDDAHERAQR
                     DRYARRRDVLLPAVKAAGLTVDHSEAGLYLWATRGEPCRDTVSWFAQRGILVAPGEFY
                     GPRGRQHVRIALTATDERVAAAASRLAA"
     gene            complement(9616..9942)
                     /gene="fdxA"
                     /locus_tag="VF874_05780"
     CDS             complement(9616..9942)
                     /gene="fdxA"
                     /locus_tag="VF874_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9175613.1"
                     /translation="MTYTIAEPCVDVKDKACIEECPVDCIYEGARMLYIHPDECVDCG
                     ACEPVCPVEAIYYEDDVPDQWSQYTQLNADFFEELGSPGGASKVGMTENDPQVVKDLP
                     PQGESA"
     gene            10064..10492
                     /locus_tag="VF874_05785"
     CDS             10064..10492
                     /locus_tag="VF874_05785"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9175614.1"
                     /translation="MASPPSTADFSFPIVPRYAEIDQQGVVFNAHYLTWFDEAFTGLL
                     DHVGLSYPELIGSGLDVHVVHSELDYAKPVRWRDAVRVAVACEQVGATSLTVTFTVMR
                     RTAGYDEVVAVRGRNVYVVVSTSDWGKRAVPDALRTALTS"
     gene            10489..11224
                     /locus_tag="VF874_05790"
                     /pseudo
     CDS             10489..11224
                     /locus_tag="VF874_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726287.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1839 a         3905 c         3754 g         1726 t
ORIGIN      
        1 accctccgcc tcgtcgggcg cttcgccgag ccaggcctgc gcgccggagt ccgtgagaat
       61 gtgacggcgt tcgccgtggc cgacgtcggc gggcaccggc accaccggca cgccggcgat
      121 caggcagccg gtgacggcca gcacggtcga cgcggtcggg gtggccaaca ccgcgacccg
      181 gtgcgcgccg ccgacgcgtt cggcgaccga cgtcgcggcg ccgaccaggt cgctgcggct
      241 tagcgagacg gtgtcgttcg aggagccgat ccgcaccgcg tcgccgaggt cggcgccggc
      301 gcccagcgag ggcagcaaca cgccagcgag gctactgcct gcgcgatgtt catgctgcga
      361 tgagttccgg tgcgctcatc tgtctatatc ggtatgacga tcacagcacg caacctcgac
      421 ggctacggag cgccggcgat cgaatgggac cgggttcgtg aggtcctcga cggccagctg
      481 acacaggcac cgggaacagt cccccgcaag cgggaggtgc ccccaggacc cgaccgtcac
      541 acaacgtggc tgacgacggt caaccatgac ggtagccccc acgtgatgcc ggtcggagtc
      601 atgcggcacg gcggacactg gtacttcacc tccggaccgg ccacccgcaa gtcccgaaac
      661 ctggcccgcg acccgcgatg cgtggtcagc gtcgcgacgc ttccgttcga cctcgtgatc
      721 gaggggacgg tgcagcgggt gacggaaggc gatgaattac gctctgttgc acaggttttc
      781 gtcgacaacg gatggccggc cgaggtcgac ggtgacgcgc tcacagccga gtacagcgct
      841 ccgtcggccg gtccgccgcc ctggtacgtc taccgcgtcg agccgtcaac gctgttcgcg
      901 ctcggcacgt ctgaaccgta cggcgccacg aaattcgatg tgacgtagcc gaattgtgac
      961 ctagccagcg cttgtgccac gcgatcgcaa taccgtggat ccgccgatcc aacaactaaa
     1021 ctgccggtga tgtgtgtcct tcggcggaag tggactgcgt tgctcggtgc agtcctgttt
     1081 gccgtgttca ccgcgagtcc gttttcagcc agcgctgaaa gtccttcgtg gaacggcaag
     1141 tacacgctcg tcggatacgc agaccagaag accggcaaca gcagggcggc ccgacaacct
     1201 gaggctccgt tcagcggcga gctcgtgttc gtcaccaagt gctcgggagg ctcgtgcgtc
     1261 gcgaccgtcg tcgacgggcc ccgcccgaag aacccgacgg tcccgctgcc cgagcgctac
     1321 acgtgggacg gcagtcggtg ggcgaacatc tacgactggc aatgggactg cttcatgggt
     1381 gacggcgccc cgaaggtgtg gagcccggcg cggtcgtgga cctattacgt tccgcagcca
     1441 gacggaacgc tgcgcggcac gtggatgacc gagatctacg gcggcccgtg tgagggcagc
     1501 gtgtggatgc cggtggcggc gttcccgctg cagcgtcagt gacggggtcg agccgcgaag
     1561 agcagtagcc cgaccgcgaa aaccgcgccc acgccgccgg acacaagcag cggcagccgc
     1621 cctgccgctc cccacagcag ccctgcccac aagccggcga acaggatcgc gaatccgctc
     1681 aacccctgga agacgccctg cccgctggac tgcagctcag gcggcagcag cgtagagatc
     1741 cacgccttgc cgacaccgtc gtagcaggcg gtgaaaaccc cgtacacccc gatcaacaac
     1801 cacgccaaca cggtgtcggt ggtcaacccc aatcccaggt agcctaccgc gaagaacgct
     1861 aaacccaccc cgaaaaccgt tgcgggcgtg aacttatcag cgagcatacc ggccggatag
     1921 ctggccagcg cgtacaccgc gttgtagccg acgtacgcca acaccacccc cgcgacgccg
     1981 aagccgatat cgttgagacg caacaacagc agcgcgtccg ggaagttggc caggctgaac
     2041 gccaccagca ccgccgccac ccgccagtac cgcccgggca gccgtcgaac accggcgaac
     2101 accggcgacc tcgcctcgcg ggtgcgggcg cgttcacgca ccaggaacac cagcagcacg
     2161 gaaatcgccg cggggatcac cgccagccac agcaccgggg cgatctggtg gtcaagcagc
     2221 tcgtagccgg ccagcccgag cagcgggccg acgaccgccc cggccgtgtc catcgcgcgg
     2281 tggaaaccga acacccggcc ccgcgctgcg ggctcgatgt cctcgacgag cagcgcatcg
     2341 cgaggcgcgc cgcgcaggcc cttgcccagc cggtcgacca cccgacccgc cagcacgccc
     2401 ggccatgcgg cggccgcggc gacgatcacc ttgcccagcg ccgccatgcc gtagccggtg
     2461 gcgatcagcg gacgccgcgc aaacctgtca cccagcgggc cggcggccat cttcgtcagc
     2521 gccgcggcac cctccgcagc accctcgacg gcgccgacca ccgacggcgg cgcgcccagc
     2581 acgctggtga gatagatcgg cagcagcggg tagagcagct cgctcgcagc gtcctgcaga
     2641 aacgacacca gcgagagcac ccagacgttg cgggtcagcc aggggcgccg gtgctcaacg
     2701 gtcacgtgcc gaagtgtctc gcatccgtcg ttgtcccgcg acacttaacc ctgtgcgacg
     2761 acacgccgcg acggccgcag acgattaagt ttcgacgagt cgtcagcggc cagccagcgc
     2821 ccgcaggaag aacgtcagat tcgccggccg ctcggccagc cgcctgacga agtacccata
     2881 ccactgcgtg ccgaacggta cgtacacccg caccgcgtca cccgcgtcgg cgaggcggcg
     2941 ctgctcggcg tcgcggatgc cgtacagcat ctggtattcg aaatcacccg cgccgcggcc
     3001 tgtttcacga gcgagcgcac cggccgccgc gatcaccgcc ggatcatgcg acgcgaccat
     3061 cggatagctt gaccccgaca tcaagacgtg aaggcaccgc aggtagctgt cgtccacgtc
     3121 gtcgggttcg cggtaggcca ccgaggccgg ctcgtcgtag gcgcccttgc acaaccggat
     3181 ccgcgcaccc gacgcagcga attcgcggca gtccgacagc gtccgccgca aataagcctg
     3241 cagcaccgcg ccgagccagt cgaagtccgc acgcaactcc cggacgatcg acagcgtcga
     3301 gtcggtggtg gtgtggtcct ctgcgtccac ggtcacccag acgcccgcgc gctgcgcggc
     3361 ggtgcagacc gcgtgggcgt tctccagcgc gatcttctcg ccgtcgcgcg gcagcgcctg
     3421 cccgagcgcc gacagcttca aggacacttc aagcgggcgg acgccttcgt aacgagcgtc
     3481 ggcccgtcgg cccagtgcgt cgagcagctc caggtacgcg tcgacggtgg cgtcggcggt
     3541 gtcggcgtcg gtgacatcct cgccgaggta gtcgatcgac accatacgat ccgaatcccg
     3601 aagcatcgca acagcattca atgcatcgtc gagcgactcc cctggcacga agcggtgcac
     3661 gacgcgccgc gccaccggcc agcgctcggc ggtgcgccgc agccgctccg agcggctcac
     3721 cgccaagatc gccggccgcg ctgtcgtcgt gaagaggccc accgtcaaac ctccatgtac
     3781 ggatagttgt ggtccgtcgg cggcacgaag ttctctttga tcgaccgtgg cgacgtccag
     3841 cgcagcaggt tcagcgccga gccggccttg tcgttggtgc ccgacgcccg ggatccaccg
     3901 aacggctgct gtccgacgac ggctcccgtc ggcttgtcgt tgacgtagaa gttgcctgcc
     3961 gcataacgca accggtcggc ggccgccatc accgcggcgc ggtcgtcggc gatcacggcg
     4021 ccggtcaacg catatcgcgc gccgcggtcc accacgtcga gtatctcgtc gtatccgtcg
     4081 tcgggataca cgtgcaccgc caggatcggc ccgaagtact cggtgcgaaa cgcctcgtcg
     4141 cccggatcat cggacagcag cacggtcggc cgcacgaagt agccctcgct gtcgtcgtat
     4201 tcgccgccag ccgcgatcgt cacgcccggc gcgcccttgg cgcgttcgat ggccgcgacg
     4261 ttcttcgtga aggcccgcga gtctatcagt gccccaccga aattcgacag atcggcgaca
     4321 tcgccgtagg ccagggcctc agtggccgaa aggaagtcgt cacccatccg ctgccacacc
     4381 gaccgcggga tgaacgcccg cgacgccgcc gagcacttct gtccctggta gtcgaacgcg
     4441 ccccggatca acgcggtgcg aagcacatcg ggctgcgcgg acgcatgcgc cagcacgaag
     4501 tccttgcctc cggtttcgcc gaccagccgc gggtagctct cgtagcgctc gacgtggcta
     4561 ccgacctcgc gccataggtg ctgaaacgtc gtcgtcgagc cggtgaagtg gattccggcc
     4621 agccggggat cactcagcag cacgtcagat accgcgaagc cgtcgccgcc caccagattg
     4681 atcacacccg gcggcaagcc cgcggcctcc agcagctgca tggtcaggta ggccgaaagc
     4741 atctgcgtca cagacggttt ccacaccacg gtgttgccca tcagcgtggg cgcggtgggc
     4801 aggttcgcgg cgatcgccgt gaagttgaac ggcgttatcg cgtagacgaa gccctccagc
     4861 ggccggtagt cggtgcggtt ccacactccg cgactgctaa gaggctgctg cgccagaatc
     4921 tgccgggcga acgcgacgtt gaaccgccag aagtcgacca gctcgcacgg ggcgtcgatc
     4981 tcggcctgat aagcggtctt ggattggccg agcatcgtgg ctgcggccag cttttcgcgc
     5041 cacggcccgg ccagcaggtc ggcggcccgc aggaataccg cagcgcgctc gtcgaagggc
     5101 gtcgccgccc actgcgcctt cgcggccagc gcggcctcca tcgcatcgaa cgcgtcggcg
     5161 tgggtggcat tggtcagcgt gccgagccgc gcggcatgcc ggtgcggctg gacgacgtcg
     5221 atgggctcgc cgctgcccat ccggtgtccg ccgccgatca cgtgcggcag gtcgatcggc
     5281 tcggcggcca gcgagttcag cgcggcagca aggcgcgtgc gttcggggct gttgggggcg
     5341 tagtcgtgca ccggctcgtt ggcgggcatg ggtacgtcgg tgatcgcgtc catgacagta
     5401 aggatggccg agccaggctc gccaaaggtt gtcttatcgg acaaggttcg cacggccaac
     5461 tagtagaatc agacaatatg gagtccgccc gggtggggct tggccagttg ctgttggcgc
     5521 tggaccggac gatggttacg ctcgtcgacg cgccgcgggg cctcgacatg ccggtggagt
     5581 cggtagcgct ggtcgacgcc gacgacatcc ggctcgggct ggctgtcggc gcgggcaacg
     5641 ccgacgcgtt cttcctgctg ggcgtctccg acgtcgaggc cctgcgctgg ctggaccgcc
     5701 acgcccagaa ggtgccggcc gcgatcttcg tcaaggagcc caccgacacg ctggtggcgg
     5761 gcgcgaccgc ggccggcgca gccgtcgtcg ccgtcgaacc ccgcgctcgc tgggagcggc
     5821 tctatcggct ggtcacgcac gtactggagc atcacagcga ccgtgatgat ccccgattcg
     5881 cctctggcac agaccttttc gggctcgcgc agtcggtcgc cgaacgcacc cacgggatga
     5941 tcagcatcga agacgcccag tcgcacgtgc tcgcgtactc cgcgtccaac gacgaagccg
     6001 acgagctgcg ccggctgtcg atcctcggcc gggcgggccc gccggaacat ctcgcgtgga
     6061 tcagccagtg gggtattttc gacgcgctgc gtacgggccg cgacgtggtc agggtcgcgg
     6121 agcggcccga actggggctg cgcccgcggc tggccgtcgg catccaccgc gacactccac
     6181 ccgatgctct tcgcgcaagc ggctcatcgg cccggcgccg atctggcttc ctcggcacga
     6241 tctggctgca gcagggttcg cgcccgctgg ccgacgacgc cgaggacgtg ctgcgcggcg
     6301 cggcggtgct ggccgccagg atcatgaccc ggctcgcggc gacgccgacg acgcacacgc
     6361 tgcgcgtgca ggagttgctc ggggtcacgg gtgaagatgt cgacgtgacc gccgtcgccc
     6421 gcgacttggg catcgtggcc gacgggcgcg ccgcgctgat cggcttccgc tgcgccgacg
     6481 ggccggctcc ggcgcatgcg atcgcgctga gcgccagcgc ttttcgcaac gacgcacaca
     6541 cggcgacagt cggcgacacg gtgtatgtgc tgttcccgca gaccggcgct gcgtcgtcgg
     6601 tgacgtcatg ggtgcgcggc accgtcgccg cgctggaccg ggaactcggc ctggacctgc
     6661 gcgcggtcat cgccaccgga atcgacggcc tggcgtcggt ggcggccgcg cgcgccgacg
     6721 tggaccgcgt gttcgacagc gcgggcaggc atccgggcgc gattcgcgcg gtcacatcca
     6781 ccgacgaggc gcgcaccacc gtcctactgg acgggatcgt cgcgctgatg gatgatgacc
     6841 tggtcgatcc gcggctgtgg gcgctgggcg actacgacgc agcgcacgac ggctcgttgg
     6901 tcgagacgct acgggcctac ctcgactgct tcggcgacgt cgccgcggcc ggcgacttgc
     6961 tgcacgtgca cccgaacaca gtgcgctacc gcgtgcggcg catcgagaag atcctgggca
     7021 cgtcgctcgg ttatccggat gaccggctgg tgctggcgct gggattgcgc gctatgccgc
     7081 taaccgagag gcagccaggg cgatccgctc gtcggtcgcg gtgagcggcg ggtcgggtga
     7141 actgggccat caaggactcg gtatcgagat actcacggac caagtcgatt cgacccagat
     7201 cgttgatgcg atagacgaaa cacaccggga tcatgtactc ccgaccgatg aggcctccga
     7261 ggtcggccgg gaatggccag cgagacgcct tggcgaagtc gatgacgtct gcggtaaggg
     7321 ttccgatgtt ccagtactcc cacacgcccc attcccgcgt cgcggagttc tgaatgacca
     7381 ccggattctt gtgggaggcg aaacctcctg accaacacca cgccaagaca tccgctttgc
     7441 ccctgcgtat ctgcccgttg ttcatcatgt cccactcgac gtcgtcggcg aattgcgcct
     7501 cggcggtctt ccgatcgctg tcgttgccgt cagcgagatg ccgcagggcc gcccagaaga
     7561 actgcgaaaa gggcgttgcc gtggagccgg gggcgagatc atcgccgcca cgttgctctt
     7621 ccgtgctcgc aaacagcgca tgcatcgtgt tgcgcgccga ggcggcatcc gttgacgttg
     7681 ccatgatcaa ctcctcatca ttcgctagcg gtttgtgcgg caagcgcttt gagtccggcc
     7741 atcgcaaaga tcgtcagccg atcggcgagc tccgatgccc cctgggtact gaggtcgagt
     7801 ccggggtagt tgcgtttgac cacgttgcga tcgccgatca gcatcacttg acacagcgca
     7861 gctaccgcaa agcagccttg ctcggcgacg ggatggttct cgggcaactt catgacgtgc
     7921 ccgatcgtcc ttttcagcag gctcagtttg gctcgccgct cagaatcgga tgtcaatcgc
     7981 tcgaaggccg ccgacggggc ggccgcctcg cgaacgagaa gccggaccgt ccaggaccga
     8041 gcgggtggac ccgttagcac gtcgacggcg aggccgatga tcgcgcgcag cttcgcctga
     8101 tcgtcggccg ccgagtcaag ggcggccgat agcgcgtccc gactcggtac ccgctcgagt
     8161 gcctcggtca ggaccgcctc atacagcctg gcgaagccgc cgaagtggta gttgatggcg
     8221 gccgtgttga ccccggcgcg ttcacagatc tcgcgtcccg tcgccgaatc gaggcctttg
     8281 tccgcgaaaa cccgcccggc cgtctccaac agccgctgcc gggtgccggc gtccctgcca
     8341 ctctcgcggc gcacagacga tgccgctgcg acaaggtttc gttgccgggc catgtccaca
     8401 cgctattcgc gagccgctcc aaagtcaaat ttcaatttca aatatgactt tgacaggatc
     8461 ggcggcagcg gactcgcggc ggtctcgtct gggggtgcgc gttatgccgc taaccgagag
     8521 gcagccgcgg cgacccgctc gtcggtcgcg gtgagtgcga tccgcacgtg ttgacggccg
     8581 cgcggcccgt agaactcacc gggcgcgacc aggatcccgc gctgggcgaa ccacgacacg
     8641 gtgtcgcggc acggctcgcc gcgggtggcc cacaggtaca gccccgcctc ggagtggtca
     8701 acggtcagcc cggccgcctt gacggcaggc agcaggacat ctcggcggcg ggcgtaccgg
     8761 tcgcgctgcg cgcgctcgtg cgcgtcgtcg tccaacgccg cgaccatcgc cgcctgcacc
     8821 ggcgtcggca tcatcatccc ggcgtgcttg cgcacggcca gcaactcggc gaccacggcc
     8881 gggtcgcccg cgacgaaccc ggcccggtag ccggctaacg agctgctctt ggacagcgag
     8941 tgcgcggcca gcagcccggt gtggtcgcca ccgcagacgg cgtcgtcgag cagcgacagg
     9001 ggttgctcat cccagccgag acccagatag cactcgtcgg acaccaccag ggcgccccgc
     9061 tcgcgggccc agccgacaac cttgcgcagg tggtcgacgc ccagcacgcg gccggtcgga
     9121 ttgctcggcg agttgaggta gatcagcgcg ggccgctgcg ggccgagctg agtcagcgag
     9181 tcggcgagga gcacctgcgc accggccagc cgtgctccga cttcgtaggt cgggtacgcc
     9241 agttccggga cgacgacgat gtcgcccgcc ccgaggccga gcagcgtcgg caaccaggcg
     9301 atcagctcct tggtgccgat caccggcagc acggcgtcgg actcgaggcc gctgacttgg
     9361 tagcggcggg cgagcgccct gaccgccgcg tcgcgcagcg ccggggtgcc ggccgtcgtc
     9421 gggtagccgg gggacgcgct cgccgcggcc agcgcgtcgc ggatcagcgg agcgaccgga
     9481 tcgaccggtg ttccgacgga caggtccacg atgccgtcgg gatgcgaccg ggacagcgcc
     9541 gacgcatcgg ccagcgtgtc ccacgggaac accggtagtg acgccgacac ttcccgtcgg
     9601 cgggtgtcgg tcaggctagg cgctttcacc ctgcggcggc agatccttga cgacctgcgg
     9661 gtcgttctcg gtcatgccga ccttcgacgc gccgcccggc gagccgagct cctcgaaaaa
     9721 gtcggcgttg agctgcgtgt actgcgacca ctggtcgggc acgtcgtcct cgtagtagat
     9781 ggcctcgacg gggcacaccg gctcacaggc gccacagtcc acgcattcgt cggggtggat
     9841 gtagagcatc cgagcgccct cgtagatgca gtcgacagga cactcctcga tgcacgcctt
     9901 gtccttcacg tcgacacagg gttcggcgat cgtgtacgtc acagagctct cctcaagagt
     9961 tcttcctcga tgttcctgtg gggctgcctt gatacgtgat actagacgtt gcatctaccg
    10021 gcgctgtcgc aggccgtccc gcgtgccttg ctaggctccc gccgtggcgt cgccgccctc
    10081 gaccgcggat ttctcgttcc cgatcgtgcc ccgctacgcc gagatagacc agcagggcgt
    10141 cgttttcaac gcccactatc tgacctggtt cgacgaggcg ttcaccggct tgctcgacca
    10201 cgtcggcctc agctatccgg agctgatcgg ctccggcctc gacgtgcacg tcgtccattc
    10261 cgaactggac tacgcgaagc cggtgcgctg gcgcgacgcg gtgcgggtgg ccgtggcgtg
    10321 cgaacaagtc ggtgcgacga gcctgaccgt gacgttcacc gtgatgcggc gcaccgcggg
    10381 ctacgacgag gtggtggcgg tgcgcggccg caacgtgtac gtggtggtgt cgacgtccga
    10441 ctggggcaag cgcgccgtcc ccgacgcact gcggacggcg ctgacgtcat gacttcccct
    10501 tacccgaatc tgctgtcccc gttggacctt gggttcacca ctttgcgcaa ccgcgtgatc
    10561 atggggtcga tgcacaccgg cttggaggac cgtgcccgcg acatcgacag gctcgcagag
    10621 tatttcgcgg agcgcgctcg cggcggcgtc gggctgatca tcaccggcgg ctacgcgccg
    10681 aaccggaccg gctggctgct tccgttcgcg gcgcagatga tcagcgcgtc ggacgcccga
    10741 cggcatcggc gcatcaccga cgcggtgcac gccgccgacg ggaagatcct gctgcgggtg
    10801 ttgcatgccg gacgctatgc gtaccaaccg ctttcggtga gcgcgtcgtc aatcaaggcg
    10861 cccatcaacc cgtttcggcc gcgccggctg tccggccccg gcgtactcaa gacgatcgac
    10921 gacttcgccc gatgcgcccg gctcgcccag gacgccggat acgacggcgt cgagatcatg
    10981 ggcagcgaag gctatctgct gaaccagttc cttgcgccgc ggccaaagac cgcgctgacg
    11041 aaatcaacac ctgcatctcc tgcaaccagg cgtgcctgga ccacgcattc gtgcacaaga
    11101 cggtgtcgtg cctgctgaat ccgcgggcag ggcgcgaaac gcagctgacg atcacgccgg
    11161 tgcggcaccg tcgtcgggtg gcggtggtcg gggcaggccc cgcaggcctg gccgcagcga
    11221 tgac
//
LOCUS       DASWBF010000169         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_800459,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000169 DASWBF010000000
VERSION     DASWBF010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800459"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..822
                     /locus_tag="VF874_05795"
     CDS             157..822
                     /locus_tag="VF874_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9175615.1"
                     /translation="MSLPMLEDITEPIEGLIRIETTAKEQATPLIMEQMRSVYPHDMV
                     FGEYCTVNDYIECPPDELFDYMADTRSLEEWTYSLRGFAPTEEPGLWVAHDKLGSPDG
                     KRLGSPIFTRTVANREARTVDYHCAWDQGKHLWMIYLMRIVDAKVVFDKPGSVVLWTN
                     CHHPFYDKNPYPETAPPQRPVWVGDFWDMFGPGHLLEMKNLKAIAEYRYHNGLPVTPD
                     WMR"
     gene            830..1846
                     /locus_tag="VF874_05800"
     CDS             830..1846
                     /locus_tag="VF874_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX9175616.1"
                     /translation="MSTPPVRLIDVSTYLPGEPIPADYYAQYVEDDLRDNVMFRAPKF
                     RHHVGKDESAIDMIERAAAGLIARHGEDAVLGADVLMTHTQLPDVAVFGCGSGVADRL
                     GMRPEWVFDLHNGGCAVFVLMMKLARQLLASGAGSSALLCVAQNTAGQVFDQKTVRRK
                     AQAAVPGDGAAVGLVTLSDRSPILDVECRTYGEYAADMTISFDVPRKYWEAGPGEGCV
                     GFTEGKITKVLARGNRLVPEVAYAVCDRIGLKPKELDMLVTNQPNRVFLRNWREALEL
                     PPERHGDTFEECGNLFGAGIPINLDRAISDGRLTAGDVVMMAGFAHAGDFAGAAAVRW
                     GGRE"
     gene            1843..2877
                     /locus_tag="VF874_05805"
     CDS             1843..2877
                     /locus_tag="VF874_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9175617.1"
                     /translation="MTVFPNHDSTAAEVVGALPDAVDPFALSLNENPFPPLPAVRTAL
                     VQAIDVSNRYPEYLPNQLRSLIAGRIGVREEQVVLGAGATGVVLQVLQAVTQPGDWIV
                     LTSPTFDGYPIFAHMARLVSVSVPLDAHGHHDLDALADAASSAKVVVLCRPHNPTGTL
                     ESVADVERFLRRVPSDTVVLLDEAYVEFVAPEHRLDGPALVQRFPNVVVLRTFSKAYG
                     LAGLRIGYGFGSPELAKWLWTMQLPFGISITSAVAVAASYDSESQLCERIRFITAERR
                     YLQRRLRSMGIYSTDAHANFLYLPAAGRSWRQEFDVAGVRARHYADGGVRVTVGNRAS
                     TRAVLAALSA"
     gene            3069..>3186
                     /locus_tag="VF874_05810"
     CDS             3069..>3186
                     /locus_tag="VF874_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9175618.1"
                     /translation="MPSSPDKRDPIAQARANWQRSGWGDVADGMVAVTSVMRA"
BASE COUNT          543 a          979 c         1105 g          559 t
ORIGIN      
        1 atgcgatgcg cacggcgctg gccgcagacg ggccatcggt ggtgagcatc gaatgcgcgg
       61 ctgacgagat accgccgttc gcgccgtttc tcggcaatgc cgtcgcgaaa acccaaggct
      121 tgaacaatca tccgatgacg aaggagaacc ccagcgatgt cgctgcccat gcttgaggac
      181 atcaccgagc cgatcgaagg tctgatccgc atcgagacga ccgcaaagga gcaggccact
      241 ccgctgatca tggagcagat gcggtcggtc tatccgcacg acatggtgtt cggcgagtac
      301 tgcaccgtca acgactacat cgagtgcccg cccgacgaac tgttcgacta catggccgac
      361 acccgcagtc tcgaggagtg gacgtacagc ctgcgtgggt tcgcgcccac cgaggaacct
      421 ggtttgtggg tcgcgcacga caagcttggc agcccggacg gaaagcgttt ggggagcccg
      481 attttcaccc gtaccgtcgc caaccgcgag gcgcggaccg tcgactacca ctgcgcgtgg
      541 gaccagggca agcatctgtg gatgatctat ttgatgcgga tcgtcgacgc gaaggtggtg
      601 ttcgacaagc cggggtcggt ggtgttgtgg accaattgcc accacccgtt ctacgacaag
      661 aacccctacc cggagacggc gccgccgcag cggccggtgt gggtcggcga tttctgggac
      721 atgttcggcc ccggccacct gctggagatg aagaacctca aggcgattgc cgagtaccgc
      781 taccacaacg gactgccggt gaccccggac tggatgagat gagcaacgca tgagcacacc
      841 ccccgtcagg ctgatcgacg tctccacgta tctgcccggt gagccgatac cggccgatta
      901 ctatgcgcaa tacgtcgagg atgacctgcg cgacaacgtg atgttccgcg ctccgaagtt
      961 ccgccatcac gtcggcaagg acgagagcgc gatcgacatg atcgaacggg ccgccgccgg
     1021 cctgatcgcc cggcacggcg aggacgcggt cttgggcgcc gacgtgttga tgacgcacac
     1081 tcaactgccc gacgttgcgg tcttcggctg cggtagtggg gtcgcggatc gccttggcat
     1141 gcggccggag tgggtcttcg atctgcacaa cggcggatgt gcggtgttcg tgctgatgat
     1201 gaaactggcc cgccagttgc tggcatcggg tgcgggcagt tccgcgctgc tgtgcgtggc
     1261 gcagaacacc gccggacagg tcttcgacca gaaaacggtg cggcgcaagg cacaggcggc
     1321 ggttcccggg gacggcgcgg cggtgggctt ggtgacgctc tcggaccgct caccgatcct
     1381 cgacgtcgag tgccgcacct acggtgagta tgcggccgac atgacgatct ccttcgatgt
     1441 gccacgcaag tactgggagg ccggacccgg cgagggctgc gtcggcttca ccgagggcaa
     1501 gatcaccaag gtgctggccc gcgggaaccg gttggttccc gaggtggcgt atgcggtgtg
     1561 cgaccgaatt ggcttgaagc ccaaggagct tgacatgctg gtgacgaacc agccgaaccg
     1621 ggtgttcctg cgtaactggc gtgaggcact ggagctgccc cccgaacggc acggcgatac
     1681 gttcgaggag tgcggcaacc tgttcggcgc gggcatcccg atcaacctgg accgtgcgat
     1741 ttccgacggt cggctcaccg caggcgatgt ggtgatgatg gccggcttcg cgcacgcggg
     1801 tgatttcgcc ggcgcggcgg ctgtgcggtg gggcggccgc gagtgacggt cttcccgaac
     1861 cacgacagca cggccgccga ggtggtcggt gcgctgcccg acgcggtcga tccgtttgcg
     1921 ttgtcgctca acgagaatcc gttcccgccg ctgccggcgg tgcgcacggc gctggtccag
     1981 gcgatcgacg tgtcgaaccg gtatccggaa tacctgccga atcaactgcg cagtctcatc
     2041 gccggtcgga tcggcgtgcg agaggagcag gtggtgctcg gcgcgggcgc gacgggcgtc
     2101 gtgttgcagg tgctgcaggc ggtgacgcaa ccgggcgact ggattgtgtt gacgtcgccg
     2161 acattcgacg ggtatccgat cttcgcgcac atggcgcggc tggtttcggt gagcgtgccg
     2221 ctggacgcgc acgggcatca cgaccttgac gcactagccg atgcggcatc ttctgcgaaa
     2281 gttgtggtgc tgtgccgtcc gcacaatccg accggcaccc tggagtcggt agccgacgtg
     2341 gagcggttct tgcgtcgggt gccgtcggac accgtcgtgc tgctcgacga ggcctacgta
     2401 gagttcgtcg cacccgaaca tcgcctcgac gggccggcgc tggttcagcg cttcccgaat
     2461 gtagtggtgc tgcgcacgtt ttcgaaagcg tacgggctgg cggggctacg catcgggtac
     2521 gggttcgggt cgccagagtt ggccaagtgg ctgtggacca tgcaactgcc gttcggtatc
     2581 agcatcacca gcgccgtcgc ggtggctgcc tcgtacgact cggaaagcca actgtgcgaa
     2641 cgcattcggt tcatcactgc cgagcggcga tacctacagc gacgactgcg ttcgatgggc
     2701 atctatagta ccgacgccca cgcgaacttc ctctatctgc ccgccgccgg ccgaagctgg
     2761 cgtcaagagt ttgacgtggc cggcgtgcgg gcccgacatt atgccgacgg gggagtgcgg
     2821 gtgaccgtcg gcaaccgcgc gtcgacgcgc gcggtgctcg ccgcgctgtc tgcctgatat
     2881 tgcgccgaag ctgtcgccag cgcgggaaag ttcgagtagc gaccgcaatg gtgctgtggg
     2941 gccacaaccg acgccccagc accgcgcggt tgtcacaggg agggggcatg gtttccgcgt
     3001 tgtcgcttat taagtgcgac cccgaggtgg ccatgccgct tctgccccac agtgcggtat
     3061 gaagaggcgt gccctcctcg ccagacaagc gtgacccgat cgcgcaggcc cgcgcgaact
     3121 ggcaacggtc ggggtggggc gacgtcgccg acgggatggt cgccgtcacg tcggtgatgc
     3181 gggcgc
//
LOCUS       DASWBF010000170         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_674026,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000170 DASWBF010000000
VERSION     DASWBF010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674026"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VF874_05815"
     CDS             <1..133
                     /locus_tag="VF874_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9175619.1"
                     /translation="LMPKTMATATVDRLLHHAHVCQTSGESVRLTQAMAGKGVNALT"
     gene            314..525
                     /locus_tag="VF874_05820"
                     /pseudo
     CDS             314..525
                     /locus_tag="VF874_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215399.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
     gene            616..1374
                     /gene="sepH"
                     /locus_tag="VF874_05825"
     CDS             616..1374
                     /gene="sepH"
                     /locus_tag="VF874_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003404617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HEX9175620.1"
                     /translation="MRELKVAGLDVDGKRIICETNGPAETFVLRADERLRAAVCGDQA
                     RAGQTQIEIEVTSVLTPKEIQSRIRAGASVQQVAEATGVDVSRVKRFAHPVLLERCFA
                     AELATAAHPVLADGPAVMTLLETVTTALVARGLDHDTTTWDAWKNEDGRWTVQLAWQA
                     GRSDNRAHFRFTPGAHGGTVTPLDEAATELIDPNFDRPLRPVAALAQLDFDEAQPAEG
                     KDEPPAQPARTRRGKPPVSAWEDVLLGVRSGGQR"
     gene            complement(1459..1725)
                     /locus_tag="VF874_05830"
     CDS             complement(1459..1725)
                     /locus_tag="VF874_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175621.1"
                     /translation="MPNHAAAASMAAAPIAAADTVDPPQPGSENASATITDLNARAYD
                     DVQINWVNGIPSVSLSQCWVNGVNTAAAASSDLRTVYVDIECPR"
BASE COUNT          309 a          614 c          630 g          295 t
ORIGIN      
        1 gctgatgccc aagaccatgg ccacagccac cgtggaccgg ctcttgcacc atgcacacgt
       61 ctgccaaacc agcggggaaa gcgtgcgact cacccaggcc atggccggca agggggtgaa
      121 cgccttgacc taactacgcc aggggcacgt ccggtggccg cgagtgggca gatctcatgg
      181 ccaccaccgg gcagttttca tgaccgccac tgggcagttc ttcatgtccc ttgacagtcg
      241 ggcggtgccg gttttgaccg atttcggctc gcgcgcaaaa ctgcccgttc gacaccagcc
      301 acctagaccg gagttgcgtt cgcaggtggt cggcaccatc gacttctccg acgacgtgga
      361 cgcgagcgcc gtcgcgaaga tcctgcgggc caatggcatc gtcgacaccg agccataccg
      421 caaactcggc cgcacccact ccgggtggcg atgttcccgg ccgtcgagcc cgatgacgtc
      481 agcgcgctca cccaatgcgt cgattgggtt gtggaacgcc tgtgagcgcg gcggtgtgtc
      541 agcaccgttt ccgctggtcg ctgcgctaga gtgcgcacct gacgggctcg gcgctgcggc
      601 ccggaggagg tcggcatgcg ggaactgaaa gttgctgggc tcgacgtcga cggcaaacgc
      661 atcatctgcg aaaccaacgg ccccgcggag acctttgttc tgcgcgccga cgagcgcctg
      721 cgtgcggcgg tgtgcggtga tcaggcccgc gcgggtcaaa cccaaatcga gatcgaggtc
      781 acaagcgtgc tcactcccaa ggagattcag tcccggatcc gtgcaggggc gtcggtgcaa
      841 caggtcgccg aagccaccgg cgtcgacgtg tcgcgggtca aacgattcgc tcatccggtg
      901 ttgctcgagc gatgcttcgc cgccgagctg gcaaccgcgg cgcacccggt gctcgccgac
      961 gggcccgcgg tgatgacgct gctggaaacc gtgacgacgg cgctggtggc ccgggggctc
     1021 gaccacgaca ccacgacctg ggacgcgtgg aagaacgaag acggccggtg gacggtgcag
     1081 ctggcgtggc aggcgggccg ctcggacaac cgcgcacact tccggttcac gcccggcgct
     1141 cacggcggca ccgtgacccc gctcgacgaa gccgcgaccg agctcatcga cccgaacttc
     1201 gaccgcccgc tgcgcccggt cgcggcccta gcccagctag atttcgacga ggcgcaaccc
     1261 gccgagggca aggacgagcc accggcacag ccggcccgca cgcgtcgcgg caagccgccc
     1321 gtgtcggcat gggaagacgt gctgctgggc gtgcgctcgg gaggccagcg ctaacgcgtc
     1381 gcgcgatcta gcgggtcagc accgccttgg acttcgacgg ggtgcatttc gccgagtcct
     1441 gctgtgtggt ggccctgtct agcgcgggca ctctatgtcg acgtagacgg tgcgcagatc
     1501 gctgctcgcg gcggcggctg tgttgacccc gttgacccag cactgcgaca gggagacgct
     1561 gggaataccg ttgacccagt tgatctggac gtcgtcgtag gcccgggcgt ttagatccgt
     1621 aatggtggcg ctggcgtttt cggagccggg ctgcggcgga tctaccgtgt cagcagcggc
     1681 gattggcgcc gcggccatcg atgcggcagc ggcgtggttg ggcaagcagc atgctccgaa
     1741 acacgtcgcg gcggcgctgg agttcgtcct cgctgacttt ctccaagcgc gccagccaag
     1801 ccgccgtagc ggacatcacc aaaggcccct agagcctgag tttccgat
//
LOCUS       DASWBF010000171         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1203175,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000171 DASWBF010000000
VERSION     DASWBF010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203175"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF874_05835"
     CDS             complement(<1..345)
                     /locus_tag="VF874_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175622.1"
                     /translation="MRYVVTGGTGFIGRRLVSKLLETRPDDEVGVLVRRSSLSRFERL
                     AADWGARAKPVVGDLTAPDLGLAPETLTGLAGVEHVAHCGAIYDIAAPERAQHAANVD
                     GTRSVIALAKRLD"
     gene            complement(403..1137)
                     /locus_tag="VF874_05840"
     CDS             complement(403..1137)
                     /locus_tag="VF874_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEX9175623.1"
                     /translation="MTSAPDHGELAPHPAEPHHGAVARRLNWLRAGVLGANDGIVSTA
                     GIVVGVAAATAERAPVFTAGIAGLAAGALSMAVGEYVSVSTQRDSEKALLHKERRELT
                     AQPDVELDELTGLYEAKGLSPETAHKVAEELTAHDALAAHAEVELRINPEELANPWQA
                     AVSSAVSFTLGALLPFLAILLPPPSVRIPVTVVAVLIALAITGWASARLGGAKSLRPT
                     LRVIVGGALAMAITFGIGHLIGGAVG"
     gene            1191..>2745
                     /locus_tag="VF874_05845"
     CDS             1191..>2745
                     /locus_tag="VF874_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9175624.1"
                     /translation="MTRSWWGWGNVEDALSESETRDLAARCAALLPDHDFTDHAPPDP
                     DQLELPSSRITPPAPLAGLCSSDPTDRAGHARGKAFRDVVLNLRGRLDNVPDLIARPR
                     SEQDVVDILDWCTRESIAVIPYGGGSSVVGGVEPRFDGPAVTMDLTGMGAALEVDAVS
                     RAARIQAGALGPQLEDQLRPHGVTLRHFPQSFGFSTLGGWLATRAGGHFATLYTHIDD
                     LTESVRVVTPAGISESRRLPGSGAGPSPDRMFLGSEGALGVITEAWMRLQDRPRWHTT
                     ASITFDDWITAVSATRAIAQAGLYPTNCRLLDPAEAFLNASTSVSGGLLVLAFESADH
                     PVDAWVDRALAIATEHRGTVIAQREREAGTNDASSTWRSSFLRMPYQRDALARRSVIA
                     ETFETACTWDRFEDLHTAVCHAAHSAVERVCGVGVVSCRFTHVYPDGPAPYYGVYAAG
                     RWDTLAEQWDEIKEAVSDAIITHGGTITHHHAVGRDHRPWYDRQRPEPFAGALRAMKT
                     ALDPGGIMNP"
BASE COUNT          452 a          976 c          898 g          419 t
ORIGIN      
        1 gtcgagacgc ttcgcgagcg cgatcaccga tcgggtgccg tccacgttgg cggcatgttg
       61 ggcccgctcg ggcgcggcga tgtcgtagat cgcgccgcag tgcgccacgt gttcgacacc
      121 ggccaggccc gtcagcgtct ccggagccaa gccgaggtcc ggtgcggtca agtcgccgac
      181 gacgggcttg gcgcgcgctc cccagtcggc ggccaagcgt tcgaagcggg acagcgacga
      241 gcgccgcacc agcacgccga cctcgtcgtc aggacgtgtt tccagcagtt tcgaaaccaa
      301 ccggcggccg ataaacccgg taccgccggt aacgacatag cgcatgggag ccatcgtggt
      361 ctccagcgta agcaaggtca atcagatgtg acgcggtcct ggctaaccga ccgcgccgcc
      421 gatcaaatgg ccgatgccga aggtgatcgc catggccagc gcaccgccca cgatcacccg
      481 cagggtcggg cgcagggatt tggcgccacc gaggcgggcg ctggcccacc cggtgatcgc
      541 cagcgcaatc agcaccgcga ccaccgtcac gggaatccgg acggacggcg gtggcagcag
      601 gatcgcgaga aacggcagca acgcgccaag ggtgaacgac accgccgagg agaccgccgc
      661 ctgccacgga ttcgccagct cctctggatt gatgcgcaac tcgacctcgg cgtgagccgc
      721 gagggcgtcg tgcgcagtga gctcttcggc gactttgtgc gcggtctccg gggagaggcc
      781 ctttgcttcg tagagcccgg tcaattcgtc caactcgacg tcgggttgcg cggtcagctc
      841 tctgcgttcc ttgtgcagca gcgccttctc ggagtcgcgc tgcgtgctga ccgacacgta
      901 ctcgccgacc gccatcgaca aagcgccggc agccaggccg gcgatgccgg cggtgaacac
      961 cggcgctcgt tcggcggtcg ctgccgcaac gccgaccacg atcccggcgg tcgacacgat
     1021 gccgtcgttg gcgccgagga cgccggcccg cagccagttg agcctgcgtg ccaccgcgcc
     1081 gtggtgcggc tccgccggat gcggtgctaa ttcgccatgg tcgggtgcag acgtcacggg
     1141 tagaggttaa ttcccacccc gtcgtcgaag gagcgattaa caattcgggg atgacgcgtt
     1201 cgtggtgggg ctggggcaac gtcgaagacg cgctgtcgga gagcgagacc cgcgatctcg
     1261 cggcgaggtg tgctgcgctg ctgcccgatc acgacttcac cgaccacgca ccgccggatc
     1321 ccgaccagct tgagctgccg tcgtcccgca ttaccccgcc cgctccgctg gccgggctgt
     1381 gttcctccga tccgaccgac cgtgcgggcc atgcacgcgg gaaagctttc cgcgacgtcg
     1441 ttctcaacct gcgcggacgg ctcgacaatg tccccgatct gatcgccagg ccccggagcg
     1501 agcaggacgt ggtcgacatt ctcgactggt gcacgcgcga gtcgatcgcg gtgatcccct
     1561 atggcggcgg cagttcggtg gtcggcggcg tcgaaccaag attcgacggc ccggctgtga
     1621 cgatggacct cacgggcatg ggcgcggctc tagaggtcga cgctgtcagc cgggccgcca
     1681 gaatccaggc cggcgcgctt ggcccgcaac tcgaagacca gctgcggccg catggggtca
     1741 ctctgcgcca ttttccgcaa tcgttcgggt tctccaccct tggcggatgg ttggcgactc
     1801 gcgcgggcgg acatttcgcc acgctttaca cccacatcga cgacctgacc gagtctgtgc
     1861 gcgtcgtcac ccctgccggc atcagcgaat cccggcggct ccccggatcc ggcgccggtc
     1921 cgtccccaga tcgaatgttt ctgggctcgg agggcgcgct cggcgtcatc accgaggcgt
     1981 ggatgcgtct gcaggaccgc ccgcgatggc acaccaccgc gtcgatcacg ttcgacgact
     2041 ggatcaccgc ggtctccgcg acccgagcga tcgcacaagc cggcctctac cccaccaact
     2101 gccggttgct cgacccggct gaggcgttcc tcaacgcgag cacctctgtc agcggcggac
     2161 tactggtcct ggcattcgaa tccgccgacc atcccgtgga tgcgtgggtg gaccgggcac
     2221 tggcgattgc caccgaacac cgcggcaccg tcatcgccca acgagagcgc gaggccggga
     2281 ccaacgacgc gtcgagcacc tggcggtcgt cgtttctgcg gatgccctac cagcgcgacg
     2341 cactcgcacg ccgcagtgtc atcgcggaga ccttcgaaac cgcctgcacc tgggacagat
     2401 tcgaagacct gcacaccgcc gtgtgccacg ctgcccacag cgccgtcgaa cgggtctgcg
     2461 gggtgggagt cgtcagctgc cggttcaccc acgtctaccc ggacggcccg gccccctatt
     2521 acggcgtcta cgccgccgga cgctgggaca ccctcgccga gcagtgggat gaaatcaaag
     2581 aagcggtctc cgacgcgatc attacccacg gcggcaccat cacccaccac cacgcggtcg
     2641 gccgcgacca ccggccctgg tacgaccggc aacgccccga gccgttcgcc ggcgcgctgc
     2701 gcgcgatgaa aaccgcgctc gaccccggcg gcatcatgaa ccccg
//
LOCUS       DASWBF010000172         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_674304,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000172 DASWBF010000000
VERSION     DASWBF010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674304"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..534)
                     /gene="nusB"
                     /locus_tag="VF874_05850"
     CDS             complement(70..534)
                     /gene="nusB"
                     /locus_tag="VF874_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9175625.1"
                     /translation="MPDRGRHQARKRAVDLLFEAEARGLTPAEVADVRNALAEQQDDV
                     SALNPYTVTVAQGVTQHAAHIDDLISAHLQGWTLGRLPAVDRAILRVAVWELLHANDV
                     PEPVAVDEAVELAKKLSTDDSPGFVNGVLGQIMLVTPQIRAAAAAVRGQDAS"
     gene            complement(527..1090)
                     /gene="efp"
                     /locus_tag="VF874_05855"
     CDS             complement(527..1090)
                     /gene="efp"
                     /locus_tag="VF874_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX9175626.1"
                     /translation="MASTADFKNGLVLQIDGQLWQIVEFQHVKPGKGPAFVRTKLKNV
                     LSGKVVDKTYNAGVKVETATVDRRDTTYLYRDGSDFVFMDSQDYEQHPLPESLVGDAA
                     RFLLEGMPVQVAFHEGNPLYLELPVTVELVVTHTEPGLQGDRSSAGTKPATLETGAQI
                     NVPLFINTGDRLKVDSRDGSYLGRVNA"
     gene            1273..2817
                     /locus_tag="VF874_05860"
     CDS             1273..2817
                     /locus_tag="VF874_05860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9175627.1"
                     /translation="MPLEVGTTFAGYTLVRRLGAGGMGEVYLAQHPRLPRDDALKLLG
                     SNVSADRTFRERFLREADLACKLWHPHIVGVHDRGEYEGHLWISMDFVDGADAGSLMA
                     RRYPAGMPRDLVTAVVTAVGSALDYAHRQGLLHRDVKPANIMITNIDDEAERRILLTD
                     FGIAKPFDDASGLTATNITVGTIDYTAPEQLMGADVDGRADQYSLAATAYHLLTGAPP
                     FRDSNPAVVISRHLNNQPPSLAKTRPDLAALDPVLATALAKDPNNRYATCSDFARALA
                     APPSRNAGAPTFVAPTMPATPNAPDSASSQSPTLLSPGWYSNPSGNPGTLYWDGQGWH
                     SPPLPTAGAKPKQGGSQLAPLLLVLGIAVALVVIGSMLFLAKQNRGSSPSSASTSAIT
                     TEVTVSAESPPTFTRSAFPRTTVAPPVFTTAPSQGNWTAVIVGTCDEGGTCGVKQRTE
                     PRNAAPRLLPNDLFDGANVSVVCQTVGDPRTSSGRSSNVWYQLANGAYVNSVYLDVRG
                     SGIPAC"
     gene            2828..3622
                     /locus_tag="VF874_05865"
     CDS             2828..3622
                     /locus_tag="VF874_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9175628.1"
                     /translation="MRHPARAAAALLLAITTSVVACGCTLTTSGTAAAPPPVTATPAT
                     SPPTTVATNADGWEAVGTSVQGRPIRARTLGHGPRKVLFIGGIHGDETEGAYSTAELP
                     AAFTAAGLADAVTLTILEDANPDGRAAGTRDNANGVDINRTFPAKNFDTSNGGEPLSQ
                     PESRVVFDLINRIGPDLVVVAHSWTGRQFVNFDGPARPIAERFAASTGLPVEESSSFA
                     STPGSLGSYFGRDRGFSILTIELLKGSAPQADWEKIREALLQAIAG"
     gene            complement(3619..>3771)
                     /locus_tag="VF874_05870"
     CDS             complement(3619..>3771)
                     /locus_tag="VF874_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX9175629.1"
                     /translation="ATLQVGAAVTVEPGVYLPDRGGVRIEDTLIVGDKAPELLTRFPK
                     DLAILT"
BASE COUNT          635 a         1317 c         1179 g          640 t
ORIGIN      
        1 gctgcgcccg gagattctgc aggcgtggcg cgaagccacg gtcggttgag ttgaagtgcc
       61 cctaccgggt cacgacgcat cctgcccacg cacggccgcg gcggccgcac ggatctgcgg
      121 ggtgacgagc atgatctgcc ccagcacacc gttgacgaat cccggtgagt cgtccgtcga
      181 caacttcttc gccaactcca ccgcctcgtc gacagccacg ggctcgggaa cgtcgttggc
      241 gtgcaacaac tcccacaccg cgacccgcag aattgcgcga tccaccgccg gaagccggcc
      301 cagtgtccag ccctgcagat gcgccgaaat caggtcgtcg atatgtgctg cgtgctgcgt
      361 gacgccctgc gcgaccgtga cggtgtacgg gttcagcgcc gacacgtcgt cctgctgctc
      421 tgccaaggca ttgcgcacgt cggcaacctc ggccggcgtc agtccccgcg cctcggcctc
      481 aaagagcaag tctaccgcgc gttttcgcgc ttgatgacga ccccggtcag gcattcaccc
      541 ttcccaggta gctgccgtcg cgagagtcga ccttgagcct gtcgccggtg ttgatgaaca
      601 gcggcacgtt gatctgagcg ccggtttcca gcgtcgccgg tttggttccc gcactcgacc
      661 ggtcaccctg caggcccggc tcggtgtgag tgaccaccag ctcgacggtc accggcagtt
      721 cgaggtagag cgggttgccc tcgtgaaacg ccacctgcac cggcatgccc tcgagcagga
      781 accgcgcggc gtcgccgacc aaagattcgg gcagcgggtg ctgctcgtag tcctgactgt
      841 ccatgaacac gaagtccgac ccgtcgcggt acaggtatgt ggtgtcgcgc cggtcgactg
      901 tcgcggtctc caccttcacg ccggcgttgt acgtcttgtc gacgaccttg ccggacagca
      961 cgttcttgag tttggtccgc acgaacgccg ggcccttgcc gggcttgacg tgttggaatt
     1021 cgacgatctg ccacagttgt ccgtcgatct gaaggacgag cccgttcttg aagtcggcag
     1081 tcgatgccac ggtcggttgt tctcctagct gagtcagatg gcgctgggga ccgtgccggg
     1141 ccacctcgac ggcgccaatc gccgaaaacc agtctaggta ccgacgctgc cgtcgtcgca
     1201 tctgggcacc tccggcgttc accgggctcg gatcctgtct gttaactgaa tgcctccggg
     1261 tacgatctcg acatgccgct ggaggttggc acgacgttcg ctggctacac cctcgtgcga
     1321 cgacttgggg ccggggggat gggcgaggtc tacctggctc agcatcctcg gctgccccgt
     1381 gacgatgcgt tgaagttgtt gggtagcaat gtctcggctg accgcacttt ccgtgagcgg
     1441 ttccttcgtg aagccgacct ggcctgcaag ttgtggcatc cccacatcgt cggggtacac
     1501 gaccgtggcg aatacgaagg ccatttgtgg atctccatgg acttcgtcga cggcgccgac
     1561 gccggcagcc tgatggcccg gcgatacccc gcgggcatgc cccgcgactt ggtcaccgca
     1621 gtcgttacag ccgtcggctc agcccttgac tacgcccaca ggcaaggcct gctgcaccgc
     1681 gacgtcaaac ccgccaacat catgattacc aacattgacg acgaggccga acgtcgaatc
     1741 ctgttgaccg acttcggcat agccaaaccc ttcgatgacg ccagcggact gaccgccacg
     1801 aacatcacag tcggcaccat cgactacacc gcgcccgaac agctgatggg cgctgacgtc
     1861 gacggccggg ccgaccagta ctcgctggcc gccaccgcct accacctcct caccggggcc
     1921 ccgccgtttc gagattccaa tcccgccgtc gtgatcagcc gccacctcaa caaccaaccc
     1981 ccatcgctgg ccaaaacacg gcccgacctg gccgcgctcg accctgtgct ggccactgcg
     2041 ttggccaaag accccaataa tcgatacgcg acctgcagtg acttcgcgcg tgcgctggcc
     2101 gcaccgccgt cccgaaatgc cggagcaccg acattcgtgg cgccgaccat gccggcgaca
     2161 ccgaacgcgc cggacagtgc gtcatctcag tctccgactc tgctttcgcc gggctggtat
     2221 tcgaacccga gcggcaaccc cgggacgctg tactgggacg gccagggatg gcacagtccg
     2281 ccgctaccga cagccggcgc gaagccgaag caaggtggca gccagttagc accgttgctc
     2341 ttggttctcg gcatcgccgt ggcgttagtc gtcatcggat cgatgttgtt cctggccaag
     2401 cagaacagag gatcttcccc atcgtcggcg tcgacgtccg cgatcaccac ggaggtaacc
     2461 gtctcagcag agtccccgcc aacattcact aggagcgcgt ttccaaggac gactgtggca
     2521 cctccagtgt tcactactgc accgtcacaa gggaattgga ccgcggttat cgtcggcacc
     2581 tgcgacgagg gcggcacctg cggcgtgaag cagcgcaccg aaccgcgcaa cgccgccccg
     2641 cggctgctcc cgaacgacct gttcgacggc gcgaacgtga gcgtggtctg ccagacagtt
     2701 ggcgaccctc gcacgagctc gggccgctca agcaacgtct ggtaccagct cgccaacggc
     2761 gcctacgtga actcggtcta ccttgatgtc cgcgggtcgg ggatcccggc gtgctgacgg
     2821 cggtgcggtg aggcacccgg cgcgggccgc ggccgccctc ctgctcgcca tcaccacgtc
     2881 ggtggtcgcg tgtggatgca ccttgacgac gtcgggcacg gcggccgcgc cgcccccggt
     2941 cacggcgacg cccgcgacga gtcctccgac gacggtggcg accaacgccg acggctggga
     3001 ggcggtcggc acctcggtgc agggacggcc gatccgagcg cggaccctcg gccacggccc
     3061 gcggaaggtg ctgttcatcg ggggcatcca tggcgacgag acagagggtg cgtactcgac
     3121 cgccgagttg ccggctgcct tcaccgccgc cgggctggcc gacgccgtca cgctcacgat
     3181 ccttgaggac gccaaccccg acgggcgggc ggcgggcacc cgggacaacg ccaacggggt
     3241 ggacatcaac agaaccttcc cggcgaagaa cttcgacacg tcgaacgggg gggaaccgct
     3301 gagccaaccc gagtctcgcg tcgtgttcga cttgatcaac cgcatcggtc ccgacctcgt
     3361 ggtcgtcgcc cactcgtgga ccggccgcca gttcgtcaac ttcgacggac cagcccggcc
     3421 gatcgccgag cgcttcgccg cttccaccgg gctgcccgtg gaggagtcga gctcgttcgc
     3481 ctcgacgccg gggtcgctgg gctcctactt cgggcgcgac cgcggctttt cgatcctcac
     3541 catcgagctg ctcaagggct cggccccaca agccgactgg gagaagatcc gagaagcgct
     3601 gctccaggcg atcgctggtt aagtcagaat cgccaggtcc ttcgggaacc gggtcagcag
     3661 ttccggggcc ttgtcgccca cgatcaacgt gtcctcgatg cggacaccgc cgcggtcggg
     3721 aaggtagaca ccgggctcca cggtcaccgc ggcgccgact tgcagtgttg c
//
LOCUS       DASWBF010000173         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_602404,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000173 DASWBF010000000
VERSION     DASWBF010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602404"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VF874_05875"
     CDS             complement(<1..399)
                     /locus_tag="VF874_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175630.1"
                     /translation="MEQAVARTAVRGRPIVLHRITVLIIAAPRRVLAVTALLMLVAGV
                     FAVPVTKSLSAGGFQDPSAESSRATQMLIDKFGQSDMQLIFMVTAPDGVYGPAARAAG
                     ADIAEYLNNAPHVTGVVSAWTAPPPGAGSLI"
     gene            complement(520..822)
                     /locus_tag="VF874_05880"
     CDS             complement(520..822)
                     /locus_tag="VF874_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175631.1"
                     /translation="MRKQPDGSTHDGHTDAYEQMVMTAEQIAWTHEFSDGIVRDSGDA
                     LLEFMEFAIQWRTANGLPTSAFAGTPAGENAPQAPRKVPARKAARKTPAKKAAHRP"
     gene            962..1606
                     /locus_tag="VF874_05885"
     CDS             962..1606
                     /locus_tag="VF874_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX9175632.1"
                     /translation="MLGSVVLVLVRLVELGGSAVGVRHGTEEPRYTVEQQIPGVEIRR
                     YGPRIAAETAVAASEEAARNVGFRRLARYIFGANHAAAKIAMTVPVAQQPGSVRGQKI
                     AMTAPVSQRAGADGEWVIRFFMPADKTMESLPEPDDDAIKLVTVPAETVAVRRFTGSR
                     SRRAIASQTAELMRALHDKGFEPTGPPAAWFYDPPWTIPMLRRNEVVVPVKPNP"
     gene            1620..2117
                     /locus_tag="VF874_05890"
     CDS             1620..2117
                     /locus_tag="VF874_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX9175633.1"
                     /translation="MRISTLIATGTSVAAAAAVGSLASRPAVQSSWYARLRKPRYQPP
                     EIVFPVVWPMLYADIAVVSASTIDELHDSGDARKLRAYQAALGLNLVLNGGWSWLFFN
                     RRAFAASAAVAAALAASSADLTRRAIAVRGAGAVPLTLYPIWCSFAAVLSTHIWLLNR
                     RRSRV"
     gene            2114..3159
                     /gene="fni"
                     /locus_tag="VF874_05895"
                     /pseudo
     CDS             2114..3159
                     /gene="fni"
                     /locus_tag="VF874_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
     gene            3176..>3517
                     /locus_tag="VF874_05900"
     CDS             3176..>3517
                     /locus_tag="VF874_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9175634.1"
                     /translation="MAVILAYGSPSLGHLFPIGALLRELARRGHQVHLRTMAAEVTSM
                     RKVGFLAEPVDPRIEAIAGQDWLACNALGVLKMSIDVHCRRAVLEVDDLNAAIDRVEP
                     DLLIVDANCWGA"
BASE COUNT          575 a         1144 c         1180 g          618 t
ORIGIN      
        1 gatgaggctg cccgcgcccg gcggcggtgc cgtccaggcc gagaccaccc ccgtcacatg
       61 cggcgcgttg ttgaggtatt cggcgatgtc cgcgcccgcc gcgcgggcgg ctgggccata
      121 cactccgtcg ggcgcagtga ccatgaagat cagctgcatg tccgactgcc cgaacttgtc
      181 aatcagcatt tgcgtcgccc gtgaggactc cgcggaaggg tcctgaaacc cgccggccga
      241 cagactcttg gtcaccggca cggcgaacac cccggcgacg agcatcagga gcgcggtgac
      301 ggcgaggact cgacgcggag cggcgatgat cagcacggtg atccggtgca gcacgatcgg
      361 cctccctcgc acggctgtcc tggcgacggc ctgttccatg tcatgcattc ggcgccgaca
      421 gccgttcggt tcggtgccat ggtgccgacc gctgctccgg ggtcgagacc gtcggcgaac
      481 ccagcaccgc gtacctgtcc tcggtgagtt ctgccgagtt cagggtcggt gggcggcctt
      541 ctttgccggg gtcttcctcg cggccttcct ggcgggaact tttctgggtg cctgcggcgc
      601 gttctccccc gccggcgtgc cggcgaacgc ggacgtgggc aggccgttgg cagttcgcca
      661 ctggatcgcg aactccataa actccagcaa cgcgtcaccg gaatcccgca cgatgccgtc
      721 ggagaattca tgcgtccagg cgatctgctc ggccgtcatg accatctgtt cgtaagcatc
      781 ggtgtggccg tcgtgcgtgc tgccgtcggg ctgcttgcgc acccagcgcc ccctgattga
      841 cgccatgcga cggtttcagg cgagttcaaa gcaccgagtc ctaaagccac aacgaccgac
      901 gaagtgccct agccgtagcc tgggcagcac agaacaatcg acagttcgtg aggaggtaac
      961 catgctggga tcggtcgtct tggtgcttgt ccggctggtc gaactgggcg gttctgcggt
     1021 gggagtacgc catgggaccg aggagccgcg ctacaccgtc gagcagcaga tccctggcgt
     1081 ggagatccga cgctacggcc cccgaatcgc cgccgagacg gcagtggcgg ccagcgagga
     1141 ggcggcgcgc aacgtaggtt tccgccgcct ggcccgatac attttcgggg cgaaccacgc
     1201 cgccgcgaag atcgcgatga cggttccggt cgcgcaacag ccgggcagcg tgcgtggcca
     1261 gaagatcgcg atgacggcac cggtgtcaca gcgggccggc gccgacggcg aatgggtgat
     1321 ccggtttttc atgcctgccg acaagaccat ggaatcgctt cccgagcccg acgatgacgc
     1381 gatcaagctg gtcaccgtcc cggccgagac cgtcgcggtg cgtcgcttca cgggcagccg
     1441 cagtcggcgg gcgatcgcat cgcagaccgc tgaattgatg cgcgccctgc acgacaaagg
     1501 ctttgagccg accggcccac cggccgcgtg gttctacgat ccgccgtgga cgattccgat
     1561 gttgcggcga aacgaggttg tcgtaccggt caagcccaac ccgtgaggag aggattgatg
     1621 tgcggatttc tacgttgatc gccaccggca cgtcagtcgc tgccgccgcg gcggtgggca
     1681 gcctggcgag tagaccagca gtccagtcga gttggtatgc gcggctgcgt aagccgcgct
     1741 accaaccacc ggaaatagtg tttccggtgg tatggccgat gctctacgcc gacatcgcgg
     1801 tggtttcggc atcgacgatc gatgagcttc acgacagcgg agacgctcgg aagctgcgcg
     1861 cctaccaggc ggccctcggc ctcaacctcg tcctcaatgg cggctggtcg tggctattct
     1921 tcaaccggcg cgctttcgca gcatcggcgg cggttgccgc cgcactggcc gcgagcagcg
     1981 cggacttgac ccgccgggca atcgccgtcc ggggggccgg cgcggtgccg ctgaccctct
     2041 acccgatctg gtgtagcttc gccgccgtcc tgtccaccca catctggctg ctcaaccgcc
     2101 gccggagtcg cgtatgacgg tggaccccgg cggaatgaca gcccgcaaac gccgccacat
     2161 cgacgtttgc cttaccgatg cagtcggcta ccacggtgtg acgaccgggc tggaacgtta
     2221 ccggctgccc tacaacgcgc tgacgcaaac ccgccttgag gatgtcgacc tgtccatcaa
     2281 ttttctcggc aaacgcctgc gggccccggt gctggtcggc gcgatgaccg gcggctcgga
     2341 gctgtcgggt gtcatcaatc ggaacctggc cgcagccgct caacggctgg acatcgggat
     2401 gatggtcggc tcgcagcgca tcatgttgga cagcacgctc ggcgagcagg ccgcggccag
     2461 cttcgccgtc cgcgacgtgg caccggatat tttgttgttc ggcaatatcg ggctctcaca
     2521 gctgagcaac gccgccgtcc ccgacatcgc cagggcgccg accgggttgg agcggatgac
     2581 cttgcggtgc atacgaatcc gctgcaggag gcaatacaac acaacggtta cactgacttc
     2641 tccggctctc ttgcccggct gcgtgacgta gcgggggaac tccgctgtcc gactctgttg
     2701 aaagaggtcg gccacggcat cggcgcccgt gcggtggccg aattgatgcg gctgtcaggt
     2761 gagctgccag tggccgccat cgacgtcgcg ggagcaggag ggacatcgtg gtcgcgcgtg
     2821 gagcagctgg tccgctacgg cgagctgcgc taccccggac ctggctgact ggggtattcc
     2881 gaccgcacaa gcgatcgtgg aggtgcgtgc ggcgctgccg gacatccgct ggtcgcttcc
     2941 ggtggcatcc gcaccggcat ggatgcggcc gaggtgatcg cattgggcgc gaatgtggtg
     3001 gcagtccccc ggccactgct caccggggcg atcgaatccg ctgccgccgc gctcgattgg
     3061 ttgcaccgct ttatcgaaga gctgcgcatc tgcctgcacg gccgcggcgc agccgacttg
     3121 ccagccttgc gcggtatcgg tgtgacatca atcccctaac cgcgagaatc gagacgtggc
     3181 agtaatcctg gcttacggat cgccgtcgct gggccatctg tttccgatcg gtgcactgct
     3241 gcgcgagctg gccaggcgcg gtcaccaggt tcacttgcgc acgatggccg ccgaggtcac
     3301 cagcatgcga aaggtcggct ttctcgccga gccggtggac ccgcggattg aggccatcgc
     3361 cgggcaggat tggctagcgt gcaacgcatt aggcgtgctg aagatgtcaa tcgacgtgca
     3421 ttgtcggcga gcagtgctcg aagtcgacga cctgaacgca gcgatcgacc gcgtggaacc
     3481 cgatctgctc atcgtcgatg cgaactgctg gggcgca
//
LOCUS       DASWBF010000174        39127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1064736,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000174 DASWBF010000000
VERSION     DASWBF010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 39127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39127
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064736"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1377
                     /locus_tag="VF874_05905"
     CDS             121..1377
                     /locus_tag="VF874_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020724870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175635.1"
                     /translation="MTEHDCDDSGPQQADASRSFPPGPHPVRHIVTPFARAGRFYAQS
                     WQEYLGRQASELPVARPTLALAAQAFIDEFVMMSFRLVRRMGDPQGFDRIEDEAAAAL
                     EFYRRTGWLDNPEGFFASPPPLTDVTVRHKQDRRRSHERIRFDSGYAPNPGEPGAERW
                     LSYTGNDREYALVLRHDEPRPWLVCIHGSAMGRAAIDLRLFRAWHLHEDLGLNVVLPV
                     LPMHGPRRRGLPDGASWPGEDVLDNVHATGQAVWDIRRLLSWIRLQEPGSPIGLNSMS
                     LGGYIASLVASLEDDLTCAILGVPVVNLFEMLGHHGAGMLSRDDPRTQLMEKVAPIGR
                     MLSPLSLAPRVPMQGRFVYAGVADQIVHPRAHAVRLWEHWGRPQAHWYRGGHTGFFRS
                     RPVQEFIDDALAQSGLIQPSQTSAGV"
     gene            1454..1909
                     /locus_tag="VF874_05910"
     CDS             1454..1909
                     /locus_tag="VF874_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX9175636.1"
                     /translation="MTSTPDYAEELRLADLRVTRPRIAVLEAVHAHPHADTETIFGAV
                     RAAVPDVSRQAVYDVLHALTATGLLRRIQPSKSVARYESRVGDNHHHVVCRSCGVIAD
                     IDCAVGEAPCLTPSDDGGVLDGYVLDEAEVIYWGLCPDCSTSETSRSHP"
     gene            1943..4174
                     /gene="katG"
                     /locus_tag="VF874_05915"
     CDS             1943..4174
                     /gene="katG"
                     /locus_tag="VF874_05915"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006437994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX9175637.1"
                     /translation="MTSDSRPPSPDVQTSSKSESENPVIPSPTPKAHAPMTNQDWWPN
                     QINVATLHSQSEKATPLGADFDYKEEFKKLDVEALKRDVIALMTNSQDWWPADYGHYG
                     GLFIRMSWHAAGTYRIHDGRGGGGQGAQRFAPINSWPDNVSLDKARRLLWPIKKKYGN
                     KISWADLIIFAGNVAYESMGLKTFGFAFGREDIWEPEEIDFGLEDTWKGTDRRYSGDR
                     ELANPLGATTMGLIYVNPEGPEGQPDPLAAAKDIRETFSRMAMNDEETAALIVGGHTV
                     GKTHGAGDASLVGPEPEAAPIEEQGLGWKCAFASGKAGDTITSGLEVVWTNTPTQWDN
                     SFLEILYGYEWELTKSPAGAWQFIAKDGAGAGTIPDPFGGPGRAPTMLVTDITMRVDP
                     IYGKITRRWLDHPEELEEAFAKAWYKLLHRDMGPVSRYLGPWIAEPQLWQDPVPTVDH
                     ELIDEQDVATLKSKVLESGLSVAQLVKTAWSSAASFRGTDKRGGANGARIRLEPQKNW
                     EVNEPAELAKVLPVLEKIQQEFNSSASGGKKVSLADLIVLAGSAAVEKAAKDAGFDIT
                     VPFAPGRTDASQEQTDMESFAVLEPRADGFRNYLRPGEKTQVERLLLERAYMLNLTAP
                     ELTVLIGGLRALGANFGGSKHGVFTDRPGTLTNDFFVNLLDMGTEWKVSESSENVYEG
                     RDRASGQAKWTATANDLVFGSHSVLRALAEVYAEDDAKDKFVQDFVSAWVKVMNNDRF
                     DLA"
     gene            4250..5314
                     /locus_tag="VF874_05920"
     CDS             4250..5314
                     /locus_tag="VF874_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003908921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HEX9175638.1"
                     /translation="MTPSQLAERVERAAEAALAEQRYVSAIDVLLGLGWLAHSHVDRW
                     RQGRIEYLDSVIQAKPTKVASAMSAFHRWAQDQKLNPSETDYVARTRDRRQLRFSATE
                     DAAVERMYRTHWLSPELSEGAVKRQSKPPDLVVISPLKEWKCASCGGTGDLLFMEDAG
                     PLCLDCADLGHLVFLPSGDAALTRRAKKASRLSAVVVRWSRSRKRYERQGILAEAEAV
                     ERAEQDCLSDAEVRARRRERDEARRADDDVKLQAEFAAAIRAQFPGCAVSRAEAIAHH
                     AATRGSGRVGRSAAGRALDPDAVRLAVAASVRHVDTDYDELLMSGVDRETARHRVGQR
                     VEEVLRGWRAGEAATPSPRD"
     gene            5355..5585
                     /locus_tag="VF874_05925"
     CDS             5355..5585
                     /locus_tag="VF874_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX9175639.1"
                     /translation="MKLSVSLSDEDVAVLDAYVQRAGLSSRSAGLQRAIRMLRHPTLE
                     DDYDNAWAEWRSAGDEEEWENSLGDGLTDAAR"
     gene            5569..5928
                     /locus_tag="VF874_05930"
     CDS             5569..5928
                     /locus_tag="VF874_05930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX9175640.1"
                     /translation="MMQRGEIWQVDLDPVRGSEANKRRPAVIVSNDRANATATRLGRG
                     VVTVVPVTGTIAKIYPFQVFLRAATTGLTTDSKAQAEQIRSVATERLIRRIGRISPVE
                     LAELDEALRLHLDLWSP"
     gene            6040..7254
                     /locus_tag="VF874_05935"
     CDS             6040..7254
                     /locus_tag="VF874_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HEX9175641.1"
                     /translation="MRALHDPEPVPLPGPGVAPTSPQPLSVLLVEDDRADALLVEELI
                     ADAAVDIRLVWAHSVADAEREIARARPDCVLLDLNLPDANGIDALDHICDCDATLAIV
                     VLTGLFDEHFGVSAVASGAQDYLVKGRVEPDMLRRSLLYAIERKRAELTAVDLHASQI
                     RARENARLERGLLPSPLLLDNPGVEIVAQYRPSRENALLGGDFYDFVQTPDRTVHVMI
                     GDVAGHGPDEAALGVALRIAWRTLTFAGLRGSDRMRQLERILHAERAGKGIFATVLSL
                     AIPPDSSRITAVRAGHPGMLLHGGGTVEWIEPPGGPALGLHTGDWAPQQVEWPEGKGL
                     ILLTDGLFEGHAGRGNERLGEQGLLELARSLATLPGPEFVDALINNAEQRAQAHGGLT
                     DDIAVVRVERTT"
     gene            7353..8870
                     /locus_tag="VF874_05940"
     CDS             7353..8870
                     /locus_tag="VF874_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX9175642.1"
                     /translation="MGVLVLAGAVAGAVLLNRTDEMSRELIDDIQPARVAAYRLQAAL
                     RDQETAVRGYVLAADRQFLDPYYDGQRAEQAAADDIRQLVGDRSELMTDLDVIERAAA
                     EWRTNYAEPLIAIVTRGTINVPKTDLVDRGKAEFDRLRTLFDAQNEHLASVRTAGIND
                     LDEIRAWRDRLLAGLVATFLVTAIALAVLVRSAVTRPLGVLATACRRIAAGNFGEKIT
                     PRGPKDIRSIAKDVENMRQRIVEELDTSRAAREALAEQAVELRRSNAELEQFAYVASH
                     DLQEPLRKVASFCQLLEKRYGDKLDQRGAEYIGFAVDGAKRMQVLINDLLTFSRVGRL
                     NASETEVDLDSALDAALGNLTTTIEETGAKIVRQPGQLPHVLGDPTLLAMLWQNLIGN
                     AVKFRRDGVAPRIVIDCEQRDDEWLLSVSDNGIGIAEEFVDKVFVIFQRLHGRDAYAG
                     TGIGLALCKKIVEHHGGTIWIDTSYTDGTRFRFTLPPMPATVDETAAATLEGTHL"
     gene            8867..9325
                     /locus_tag="VF874_05945"
     CDS             8867..9325
                     /locus_tag="VF874_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9175643.1"
                     /translation="MTPAGRPIDVLLIEDDPGDVLITREAFEHNKINNTLHVAHDGEE
                     GLDFLYRRGAFQDVPRPDLILLDLNLPKYDGRQLLEKIKSDPELSHIPVVVLTTSSAE
                     EDILRSYKLHANAYVTKPVDLDQFMNAVRQIDEFFVQVVRLPQPGSAGGA"
     gene            9467..9913
                     /locus_tag="VF874_05950"
     CDS             9467..9913
                     /locus_tag="VF874_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9175644.1"
                     /translation="MTATLTTPAARFDNAAFDCGPAQIRAHFRHLATVVTITGRIDAA
                     NVDPVTEYTRRFILAKDALILDLSDVNLPAAEAISFVNTVAAECRAAGVECMLVAGEA
                     VVDVLDDFDDEALLPIAGSVHEALHYFADVIATRRQLLLPLIKKTA"
     gene            complement(9910..11511)
                     /locus_tag="VF874_05955"
     CDS             complement(9910..11511)
                     /locus_tag="VF874_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9175645.1"
                     /translation="MTRYDYIIAGAGSAGCVLANRLSADPRITVLVIEAGGPNRSPYF
                     HIPKGYGKIFENENYVWRYATTPFGPNQRSEFWTRGKVLGGSSSINGLVYNRGNRADY
                     DELEHLGNKGWGWDEILPIFKAFENNGFGASPTRGVGGPLPVTVPRDPDPLCAEMVEA
                     ATKTGLTEVQDINESDTERVGYATSTIRNGRRVSAADAFLKPIRHRPNLTVITHTTVR
                     RIMFEHGRAVGVEVAGRHGTADLRAIREVIVALGSLNSPQVLQLSGIGPRDVLQRAGV
                     PVYLGRDNVGRRMREHRCMTLRFRLSEDLGYNRQLATSFGQALTAARYVASRRGPLAA
                     PSFDVLAFVKTTPDAERVDGQLLMGPFTIPAYNVGEPVAVERAPGISCLGFVLRPTSE
                     GSVTITSADPTAVLRVEPNYFASDYDRKTGANLLRKMRKVFEQSPIAGRIRHETFPGP
                     DAQSDDELVDSALDGGYCGYHAVGSCAMGPNDDDVVDSELRVRGVDNLRVVDCSVMPT
                     MVAGNLNGPVMAMAWRAADFILQGR"
     gene            11559..12038
                     /locus_tag="VF874_05960"
     CDS             11559..12038
                     /locus_tag="VF874_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175646.1"
                     /translation="MLSLGWAVRYGFVRADRSAVVIATFVLAACALSVGVASADPPAP
                     APEPKTTIDHDGTFVVGTDIVPGTYTSAGPVGSGTCYWKRMSNPDGGDIIDNALSKKP
                     QIVQILPSDGAFKTNGCQPWQITDAVPPAGVPPLIAGAQLQGIINDINARAGQSGAG"
     gene            12106..12234
                     /locus_tag="VF874_05965"
     CDS             12106..12234
                     /locus_tag="VF874_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175647.1"
                     /translation="MQQVARTAMGPLAHLVADHGLGVVVPDRAADVAARIVALNSR"
     gene            complement(12221..13585)
                     /locus_tag="VF874_05970"
     CDS             complement(12221..13585)
                     /locus_tag="VF874_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9175648.1"
                     /translation="MQNEATLDAHSDDPRYHFDRHTPEYREQFQTVTEQMQSRCPLAW
                     TDTYGGHWVAAGSEQVFELARGPHLSNDNDVNGERKGYKGIQIPQQEYSAHVRGGMLE
                     MDEPEHRLYRTVLNPYLSPAAVQRWTPVVDEIVKACLDEKIEEGRIDFVDDLANIVPA
                     VLTLALMGIPLASWQIYNEPAHAQVYTPADSPDTSRVAEMYTVMMADLLRNLAEVREV
                     PRPGLVDALVKLRINGEPAPEMELLGMLTLLIGGGFDTTTALTAHALEWLSEHPDERA
                     RLSRDRDELLDSATEEFLRFFTPAPGDGRTIAQDCEINGTRLKQGERLWVSWAMANRD
                     PSLFEDPNTVVLERGANRHFSFGLGIHRCIGSNVARIVFKRMLTAVLDRMPDYRCDPE
                     GTVHYDTIGVIQGMKHLPATFTPGPKLGPGLDVTLERLQRVCDEEGLARPITERKEPA
                     VIDY"
     gene            13654..14508
                     /locus_tag="VF874_05975"
     CDS             13654..14508
                     /locus_tag="VF874_05975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX9175649.1"
                     /translation="MTVLPDLNTVRQTLGRSLFGMVAGPDGPANRSLIHHTPGPRWFA
                     EDRPIRRVHADASMFVGGLRALLLQSLHPLAMAGVADHSDYRGDPWGRLQRTSTFLAV
                     TTFGPAADAQRAVDRIRGIHQRVRGTAPDGSAYHAADPHLLEWVHVAEVDSFLLAHRL
                     YGASPLDQSGRDGYVADTARVALALGVPDPPRSERELHERIQDFRPELRGTAAARDAA
                     RFLLFTPPLPIAARVPYGVLAATSVAMLPAWARMPLLLPYFPPVEATVIRMAGRAVVG
                     GIRWAMTG"
     gene            14505..15095
                     /locus_tag="VF874_05980"
     CDS             14505..15095
                     /locus_tag="VF874_05980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175650.1"
                     /translation="MSTRWGRVGAPRGDDYDERWRHLAAVGHNVHGEADLVEQLLRET
                     GGTRVLDAGCGTGRVAIELARRGFSVVGVDADGGMLDAARAKAPDLTWLHTDLADFSS
                     SAGTFDLVVLAGNVMIFLAPHTEGLVLQAVTASLVPGGLLVAGFAIRPHRLQLAEYDR
                     FAQAAGLVPVAQWATWDREPFTGGDYAVSVHKRPAI"
     gene            15108..16244
                     /locus_tag="VF874_05985"
     CDS             15108..16244
                     /locus_tag="VF874_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HEX9175651.1"
                     /translation="MKLPEPDSGIPHLADVVPSVLAAMGVASLEARIGLPADISGACV
                     LLIDGLGAELLDTYAADAPVLAEMRGQTLQVGFPSTTVAGLAALGTGCRSGEHGMVGY
                     SFRLPGAGLFNALRWRPHPWGEDLRDDVAPEQVQPMPTTFERAASAGVAVSVVSGAEF
                     TGSGLTRAVLRGGRYVGVHALGDLAVGVRAAIADGGFCYGYHGELDLLGHVYGPGAAA
                     WRSQLRQVDRLVESVVESLPPGGLLAVVADHGMVSVDSANAVDVDDCEQLLDGVQAIG
                     GEPRARHVYVADGAAEAVLAAWREALADRAWVVSREEAIAAGWFGERVTDDVRPRIGD
                     VVVAARGSAAVVRRKVEPLESSLIGHHGSLTTAEQRVPLLLAYG"
     gene            complement(16248..16514)
                     /locus_tag="VF874_05990"
     CDS             complement(16248..16514)
                     /locus_tag="VF874_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175652.1"
                     /translation="MQINGGPSVTVDAIETFRKTISEAAAGDNIGVLFSKLDKGAVAP
                     RRRHLLVVRSLGLRRNWGHLPLAGEIPAGPHSKFSCKYAVSTSR"
     gene            complement(16672..16848)
                     /locus_tag="VF874_05995"
     CDS             complement(16672..16848)
                     /locus_tag="VF874_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175653.1"
                     /translation="MGDLAITGEETQVRRQLERLHQIGVTDFVASRIALASDSDAYDR
                     TYQFLADFARRGLN"
     gene            17190..17417
                     /locus_tag="VF874_06000"
     CDS             17190..17417
                     /locus_tag="VF874_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175654.1"
                     /translation="MAGHVHRLIGEQWHQHLQVLTQMADRVRIIEAEAGFDNRLVAEP
                     DAKREPVRAELLDGECLLRKRRRMSRKGGTT"
     gene            complement(18125..19042)
                     /locus_tag="VF874_06005"
     CDS             complement(18125..19042)
                     /locus_tag="VF874_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175655.1"
                     /translation="MAALYRPAVVALVAAAATVLPCGRATADAGLVDIGGGRSIYLDC
                     QGDGSPTVLIIPGKGSYADVWNVVVPEHDPIRSSPYDLIEQAELEPSPTATQPTVART
                     TRVCAYDRPDTRPDGMNQSTPVPQPHTVAQDVDDIVKLIAAADISTPVVVAAHSYGGL
                     VADLLARTHPELVSGLVLVDPVSEFLPTLGSPQQNATFNHDAAVPEDVGGEGFLADDA
                     YARIAAAPPLPQVPAVVLGSDKFPPPAQLKPDNYTLAQIEQANTLLADALGTVNLTTT
                     DSGHNVMLYQPQLVADEIVTIVERVRAGA"
     gene            19377..19751
                     /locus_tag="VF874_06010"
     CDS             19377..19751
                     /locus_tag="VF874_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175656.1"
                     /translation="MGGKDLDVYSFYAKDPKVPWPYRRHGKADFGESEFGYYRPAGRP
                     YEYKGERFVGRPVDLLGRALPVEPDADPVQAVRDWLATSNNATPRLLRLKAVVGLYPA
                     NIAARSRYRSNGGRKAAALTRR"
     gene            complement(19738..21109)
                     /locus_tag="VF874_06015"
                     /pseudo
     CDS             complement(19738..21109)
                     /locus_tag="VF874_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774059.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            21548..21694
                     /locus_tag="VF874_06020"
     CDS             21548..21694
                     /locus_tag="VF874_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9175657.1"
                     /translation="MWKLDRLGRNTLNILETVKALTDQGVTLCRPPTASTPNRAAASP
                     RRAS"
     gene            complement(22095..22646)
                     /locus_tag="VF874_06025"
     CDS             complement(22095..22646)
                     /locus_tag="VF874_06025"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HEX9175658.1"
                     /translation="MRLWRVLPWEPSATVGQPGHAVWVPREYQGSGRHDAPDRYGCLY
                     LAEMAVSAVAETLAPFRGTGDLHPELLVRAGRQLALAELELAGDATLIDLDDPAVLVT
                     ESLRPSIVATGQRSVTQAYAVGQFERHTDAAGLRWWSILEASWMQVTLFDRALGGLSV
                     RGVRTLAVDDEAVTVAASYLGLA"
     gene            complement(22643..22975)
                     /locus_tag="VF874_06030"
     CDS             complement(22643..22975)
                     /locus_tag="VF874_06030"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEX9175659.1"
                     /translation="MPVSTKEKVIALSRDFGSQRRLAELLGVNAAQVTRWRRGQGIDD
                     VNAARVDLLELVMAYLLRVYSAEAAERWLVGMNPSLGGRRPVDLIRRGHAREVLDAIA
                     NERAGSFA"
     gene            complement(23337..24110)
                     /locus_tag="VF874_06035"
     CDS             complement(23337..24110)
                     /locus_tag="VF874_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175660.1"
                     /translation="MDQTITPARTRAGTTAVITGGARGFGKAFGAALAAEGAHVVLID
                     LDGAAVESAAAHIRISGGAATGYAGDVTDEGRVAAIMTEVADTHSGIDVLINNAGLHS
                     DEYSRPIREMGLAKTRRLFEVNVLGVISCTLAAQPYMAGRDGANIVNIASAAAHMGGT
                     AYGTSKLAVAGLTITFARELGTDGIRVNAISPGMILTETIRAELPEATKAQIKAMQFI
                     DADGTETNVVEAMLFLTSDRARFITGETLRVTGGMAAGV"
     gene            24655..25152
                     /locus_tag="VF874_06040"
     CDS             24655..25152
                     /locus_tag="VF874_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX9175661.1"
                     /translation="MSAAPIQDIPLTTLDGNPISLSQFADRAVLVVNVASKCGLTPQY
                     TALQKLAGDYVDRGLTVIGVPCNQFMGQEPGSAEEIQTFCSTTYGVTFPLLEKTDVNG
                     EGRHPLYEELTKTPDSDGAAGDIQWNFEKFLLAPGGRVVNRFRPRTQPDAPEVVAAIE
                     AVLPG"
     gene            25183..26100
                     /locus_tag="VF874_06045"
     CDS             25183..26100
                     /locus_tag="VF874_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175662.1"
                     /translation="MVVAIARPKLEGNVAVGENRQIGFAEFGAPQGRAIFWLHGTPGA
                     RRQIPTEARVFAEQENIRLIGLDRPGIGSSTPYQYETVSMFAEDMRTVADTLGIDKMA
                     VVGLSGGGPYTLACAAKMPERVVVAGVVGGVAPACGPDRIGGGLMALGSRIAPLLEVA
                     GAPIRLTALTLVRLIRPVAGPAAGLYGLVSPPGDRRLLARPEIKAMFLDDLLNGSRKQ
                     LAAPFYDVVAFARDWGFRLDEVKVPVRWWHGDADHIIPFSHGQHVVGRLPDAEFYPMP
                     GESHLGGLGRAEEILRTMIETWDKDALRP"
     gene            complement(26124..26672)
                     /locus_tag="VF874_06050"
     CDS             complement(26124..26672)
                     /locus_tag="VF874_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175663.1"
                     /translation="MRNSIVSTGTLTATAALTVAAAAFGIGSATAQPDDYTVLLIDPN
                     AVTDSTAYTAGAPVFNPNNQPGVEAVYTHRDGSRQITETVWVLQDAAAATAALNAAQA
                     DAAGQVANETTQPVQVGTGGTLLSGTSADGSKSISVLLFTQGNTASAIEFAGPPTDPA
                     PAELVTEIGPKQDAVIQSALGA"
     gene            complement(27073..28338)
                     /locus_tag="VF874_06055"
     CDS             complement(27073..28338)
                     /locus_tag="VF874_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX9175664.1"
                     /translation="MSARAGIVVTGTEVLTGRVQDRNGPWIADRLLELGVELAHITIC
                     GDRPGDIEAQLRFLADEGVDLIVTSGGLGPTADDMTVATVARFCGRELVLDAQLEDKI
                     TDILKRLMARFANVDLDAVRAANRKQAMIPTGSHVIDPVGTAPGVVVPFKPTVVVLPG
                     PPRELQPMWPKAMQTDAVQEALAGRTTYRQETVRMFGLPESGLAETLREAEQDVPGFE
                     RLEITTCLRRGEIEMVTRYEPDATGVYANLMEVLRQRHGREIFSEDGSRVDDQVATLL
                     TGRRIATAESCTAGLMAARLTERPGSSDYVVGGVVAYANEAKTEVLGVDATLIERHGA
                     VSEPVAESMAEGALRRFDADTAIAITGVAGPGGGTEDKPVGTVCFSVMLAGGPTQTRT
                     LRLPGNRSDVRERSTTVGMHLLRRALLDR"
     gene            28370..28864
                     /locus_tag="VF874_06060"
     CDS             28370..28864
                     /locus_tag="VF874_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX9175665.1"
                     /translation="MIDIEVQQADVTKLEVDAIANAANTQLRHGGGVAAAIARAGGPD
                     VQRESNEKAPIGLGEAVETTAGEMPARWVIHAATMELGGPTSSEIISRATRSTVRKAD
                     ELGCRSLALVAFGTGVGGFPLDEAARLMVGAVRKHEPQSLSSVVFAVHGAAAEQAFRA
                     ALDS"
     gene            29133..29402
                     /locus_tag="VF874_06065"
     CDS             29133..29402
                     /locus_tag="VF874_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175666.1"
                     /translation="MHALSRRERMVIVPMVWAYRFVGAAPPLANGVARALLGRAFMRT
                     RPEDAAAVVASFRNAPRSGMYRAMTSVMLNRADLDPILPQITTPR"
     gene            29402..29605
                     /locus_tag="VF874_06070"
     CDS             29402..29605
                     /locus_tag="VF874_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175667.1"
                     /translation="MVVPTADAMLPAHQIHAAVRQMPCAASIEVKAEGHVAPIIANAE
                     QLADMITAFGRDPKGYVSRQVRD"
     gene            29615..30985
                     /locus_tag="VF874_06075"
     CDS             29615..30985
                     /locus_tag="VF874_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9175668.1"
                     /translation="MAQAPRTRYARCGEMDIAYQVFGDGPLDLLVLPGPFIPIDSIED
                     EPSMYRFHRRLASFSRVIRFDVRGIGLSSRVPSLDVIGPEYWAQDAIAVLDAVGSEQA
                     TIFAPSYTATSGIVLAADYPARVHSLVIMNGAARVMWAPDYPAGTRLEASELFTTVAT
                     EPDAVEQGVDVLKVVAPSVADDQAFRAWWDRAGNRAASPSMARAVSDVVQTADVRDRL
                     ARILAPTLIMHRAGSGFVPVGHGRYLAEHITAARYVELPGADVPYWVGDTAEMLDEIE
                     EFITGMRGRFDAERVLTTIVFTDIVGSTRRAAELGDVRWHDLLDKHDSIVRHELERFR
                     GREINTAGDGFVATFISPSVAIDCAEAIVDAVRALGVEVRVGIHAGEVEVRGADVAGM
                     AVHIGARVAAHAGPSEVYVSSTVREIVTGSRRTFVDRGEYELKGVPGAWRLYRVARDH
                     MAGSVP"
     gene            30982..32448
                     /locus_tag="VF874_06080"
     CDS             30982..32448
                     /locus_tag="VF874_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEX9175669.1"
                     /translation="MSDTQQATEEPVERTLFGHPIGLANLFGVELWERFSFYGMLTIL
                     GYYLYYSVTDGGLGLPKSTATGIVGAYGGLVYLSTVLGGWIADRLLGMERTVLYGGVV
                     VMAGHIALAVVPGLPGVGMGLVLVALGSGALKANASSLLGTLYEKGDARADGGFTLFY
                     LGINLGAFIGPLITGLLQTHVGFHYGFGAAAIGMAAGLAQYVVFRRNLGTHGRDVPNP
                     LPRSAISRVAALVVAVLVLIAVVLLTGVVRLSNLSQVTTGVIVVASAAYFVIMLTNSR
                     VTPTERSRVRAFIPLFVANAVFWSLFQQIFTVLAVYSDERMNWSIFDWTAPSNWIGSI
                     EPVWIIVLSPVFAIMWTRLGTRAPTTPRKFAYGVIGMGLAFLLFLPMAGTTGKAVPAL
                     LVVGIMAVFAISELLISPIGLAVTTKLAPEAFRAQMMALYFFSVGLGTSMSGVLARYY
                     DPDHEFAYFGIIGAVAIVAGVVVFALTPWVSRRMEGVH"
     gene            complement(32566..33306)
                     /locus_tag="VF874_06085"
     CDS             complement(32566..33306)
                     /locus_tag="VF874_06085"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9175670.1"
                     /translation="MGRVDEKVALISGGARGMGAAHARMLVAEGAKVVIGDILDDEGK
                     ALADDLGDAARYVHLDVTDPEQWKAAVSTAVDEFGKLNVLVNNAGTVALGPLRTHDLK
                     KWQQVLDVNLTGTFLGMQAVIDPMTEAGGGSIINISSIEGLRGAPWVHGYVASKWAVR
                     GLTKSAALELAAQKIRVNSIHPGFIRTPMTKHLPDDMVMAPMGRPGTPEEVATFVLFL
                     ASDESSFATGAEFVMDGGLVADVPHRQF"
     gene            complement(33323..34420)
                     /locus_tag="VF874_06090"
     CDS             complement(33323..34420)
                     /locus_tag="VF874_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX9175671.1"
                     /translation="MAIRVAHIGTGNVGRLALTGILTNPQFELTGLWVSSDAKVGKDA
                     GELAGLDVHTGVAATNDLDALLADKPDCAVYCAMGDNRLPAAMDDVRRILAAGVNVVG
                     SAPVLLQYPWHVLPQQYIEPLEEAARQSDSTIFINGVDPGFVTDLIPLAFAQTCQRIE
                     QVRCMEIADYATYDGATVMFDVMGFGKPLDEVPLLFQPGVLSMAWGCAIRQLAAGLGI
                     ELDEITDSCTREPAPEAFDIAAGHIAKGTVAALRFDIRGMLGGRPAIVVEHITRLRED
                     LRPDWPQPAQPGGSYRVEIVGEPSYKVDICPSSSRGDHNHAAIVAAAGRIVNAIPAVV
                     AAPPGIRTTLDLPLITGKALFSHRIATGSSC"
     gene            34454..35275
                     /locus_tag="VF874_06095"
     CDS             34454..35275
                     /locus_tag="VF874_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX9175672.1"
                     /translation="MLAVRVPVSDSVAAIAAGPAGPRVGAFFDLDGTLVSGFTATVHA
                     SDRIRRGQARVGEVLGILEASLRYRFGRMQFERLLVRAAGYLQGESIHELDELGEQLF
                     SRHVAARVYPHMREIVQAHQQRGHTVVLSSSALTIHAEPVARFLGISRVVCNQFELDE
                     HGALTGQIVRPVVWGSQKATAVQRLCVANDIDLQRSYFYADGDEDIALMSLVGHPRPV
                     NPRPGLAAMAAQRGWPVLRVTSPDGRTRARSLRTVASVGWSALMRYATSTTLRRE"
     gene            35275..35742
                     /locus_tag="VF874_06100"
     CDS             35275..35742
                     /locus_tag="VF874_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175673.1"
                     /translation="MSVATLLPAACAGFLLAVLWMDLIFDVQVVAHRRSLGDLPESVL
                     ASIAGYYRRATTRPMSMLIAVVMLMLLAALAVRGASGDPGWLIATWAALAGGPILLAL
                     IRTVPNAVRLGRRADTVADQSRLARAIFRDHVVCFALMLAFLGLWLAAASLRL"
     gene            35739..36392
                     /locus_tag="VF874_06105"
     CDS             35739..36392
                     /locus_tag="VF874_06105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175674.1"
                     /translation="MTTTLRDPKVAHALDRMYREATEQMTARQWPDLQSSASAQERAD
                     ALSDFYLPVTPEAGRLLYSLVRAARPGTIVEFGMSLGISAIHLAAAVRDNGHGQVVTT
                     ELSAAKVAAAKKTFADIGLDDLITVLEGDALSTLEAVDGSVDFVLLDGWKELYLPVVK
                     LLEPRLSPGALIVADNTEMADTRSYLDHVRDPDNGYVSVNFRARDADSMEITCRATT"
     gene            complement(36393..37877)
                     /locus_tag="VF874_06110"
     CDS             complement(36393..37877)
                     /locus_tag="VF874_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017836716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9175675.1"
                     /translation="MDEREYTQLDALGLAELVASGEVQAAELLRIAQARADRFGPKIN
                     AICTRLDQRAEQRVSGELHGPFAGVPFLLKDLHQDLAGTTTTDGCRALADRTVFDNST
                     VVQRWLDAGVVIFGKTNTPEFGAKGITEPELFGPTRNPWDLDRTPGGSSGGAAAAVAA
                     GIIPVAGASDGGGSIRIPAACTGLVGLKAGRGLIPSGPDASDPLNGQATNGVISRSVR
                     DTAAMLDVLAGPDAVSAFAPATPDRPFLDDLNRPPDRLRVGFTTHSAIRGSAHPEATR
                     AVEAAAQLLAELGHDVEQVAPSWDDHQLAHDFLTVWFVAQAIEIARIKRETGAKDDGF
                     EQDTRMMAALGRAVSGVALQEAQERRHDHIAGLAQLHASYDLLMTPTLGEPPIRIGSL
                     ELPAVLRTIANGFLRTRTVRILESTGIADQIIRRNLSWVPYTQLANITGRPAISLPLH
                     WTASGLPMGVQFVAPLSGEKLLLRLARQLELTAPWAQRRPPQFT"
     gene            complement(37976..38545)
                     /locus_tag="VF874_06115"
     CDS             complement(37976..38545)
                     /locus_tag="VF874_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175676.1"
                     /translation="MSNFRNTPSFGLGLDVNWSGEHMSRIGTRIATIAAAAGGLVTAF
                     VALSPTAGADSAPAVPGMPGIDLMQQLGNAPAMASQFLQNAASMLAPKPAVSAPAVSA
                     PAATTPTPISAPMNSLVGTVPQGQLNLPASVPMAPLLNQLGLPGNLSSLPIPNLGANP
                     GAPITAPAVPAAPGQPALPPNLNPFSALP"
     gene            38773..>39127
                     /locus_tag="VF874_06120"
     CDS             38773..>39127
                     /locus_tag="VF874_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9175677.1"
                     /translation="MHTAICDELGIEFPIFAFTHCRDVVVAVSKAGGFGVLGAVGFTP
                     EQLEIELNWIDEHIGDHSYGVDIVIPNKYEGMDSNLSAEELTKTLQSMVPQEHLDFAR
                     KILADHGVPVEDSDQD"
BASE COUNT         6532 a        12618 c        12966 g         7011 t
ORIGIN      
        1 ctacgcaaat ttgccgagcg gtacgcgcga ccgagtgacg accgctgagt tcgggtcagg
       61 gtctgaggcc tttcacctta gtccctagcg ggtcgcgtct ggtcgtccta gcgtcgccgg
      121 atgacagaac atgactgcga cgacagcggc ccgcaacagg cggacgcttc tcgttcgttc
      181 ccgccgggtc ctcatccggt gcgccacatc gtcacgccgt tcgcgcgagc cggccgcttc
      241 tatgcgcagt catggcagga atatctcggt cgtcaggcca gcgagcttcc agttgcgcgg
      301 cccaccctcg cgctcgcggc tcaagcgttc atcgacgagt tcgtcatgat gagttttcgg
      361 ctggttcgcc ggatgggcga cccgcagggg ttcgaccgaa tcgaagacga agcagccgcg
      421 gcgctggagt tctaccggcg gactgggtgg ctggacaacc cagagggctt ctttgcgtca
      481 ccgccaccgt tgaccgacgt caccgtgcgt cacaaacagg accgcaggcg ctcccacgaa
      541 cggatccgct tcgacagcgg ctatgcgccg aacccaggtg aaccaggcgc agaacggtgg
      601 ctgagctata ccgggaacga tcgcgaatac gcgctggtgt tgcgtcacga cgaaccgcgt
      661 ccctggctcg tgtgcataca cggcagtgca atgggcaggg ccgcaatcga tctcaggcta
      721 ttccgcgcct ggcacctgca cgaggatctc ggtctgaacg tcgtcctgcc cgtcctgccg
      781 atgcacggtc cccgcaggcg cgggctgccc gacggagcgt cgtggccggg tgaggatgtg
      841 ctcgacaatg tgcacgcgac gggccaagcg gtgtgggaca tccggcggct gctgtcgtgg
      901 atacgactgc aggagccggg ttcgccgatc gggctcaaca gcatgtcgct gggcggatac
      961 atcgcgtcgc tggtggccag cctcgaagac gatctgacct gcgcgatcct cggcgtgccg
     1021 gtggtgaatt tgttcgaaat gcttgggcac catggcgccg gcatgttgtc tcgggacgat
     1081 ccgcgcactc agctgatgga gaaggtggcg cccatcgggc ggatgttgtc gccactgtcg
     1141 cttgctccgc gcgtgccgat gcagggccgc ttcgtctacg ccggcgttgc cgaccaaatt
     1201 gtgcatccgc gcgcgcatgc cgtccgcctc tgggagcact ggggcagacc acaagcccac
     1261 tggtaccgcg gcggtcacac cgggttcttt cgctcccggc cggtgcagga gttcattgac
     1321 gacgcgctcg cgcagtcagg gctgatccag ccgtcccaaa ctagtgccgg agtgtgacgg
     1381 gccggggccg gccttgccac atcgtcgctc tgaagtcttg actgagtcca gaaaagtggg
     1441 gcatattgtc tgcgtgacgt cgacgccgga ttacgcggaa gagctgcgcc tggctgacct
     1501 tcgcgtgacg cgcccgcgga tcgcggtgct ggaggcggtg cacgcgcatc cgcacgccga
     1561 caccgagacg atcttcggcg ccgtgcgcgc cgccgtaccc gacgtctccc ggcaggccgt
     1621 ctacgatgtg ctgcatgccc tgaccgccac tggactgctg cgccgaattc agccgtccaa
     1681 gtccgtcgct cgctacgagt cgcgtgtcgg cgacaaccac catcacgtcg tctgccggtc
     1741 ctgcggggtc atcgccgaca tcgactgtgc cgtcggtgag gcgccgtgcc tgacgccatc
     1801 cgacgacggt ggcgttctgg atggctacgt cctcgacgag gccgaggtca tctactgggg
     1861 tttatgcccc gattgctcga catccgagac gtcgcgatca cacccgtgat cacagcccga
     1921 ttcacccacc gaaaggaatg cagtgacatc cgatagccgc cccccctccc cggacgtaca
     1981 gacttctagc aagagcgaaa gcgagaaccc ggtcatcccg tcgcccacgc ccaaggcgca
     2041 cgctccgatg acgaaccagg actggtggcc caaccagatc aacgtggcga ccctgcattc
     2101 gcagtcggaa aaggccactc cgctgggcgc ggacttcgac tacaaggaag agttcaagaa
     2161 gctcgacgtc gaggcgctga agcgcgacgt catcgcgttg atgacgaact cacaggactg
     2221 gtggcccgcc gattacggcc actacggcgg cctgttcatc cggatgagct ggcatgccgc
     2281 gggcacctac cgcatccacg acggccgcgg cggcggcggc cagggcgccc agcgcttcgc
     2341 cccgatcaac agctggcccg acaacgtgag cctggacaag gcacgccggc tgctgtggcc
     2401 gatcaagaag aagtacggca acaagatctc ctgggccgac ctgatcatct tcgccggcaa
     2461 cgtcgcctat gaatcgatgg ggctcaagac ctttggcttc gcgttcggcc gcgaggacat
     2521 ctgggagccc gaggagatcg acttcggcct cgaggacacc tggaagggca ccgacaggcg
     2581 ctactccggt gaccgggaac tggccaatcc cttaggcgcc accaccatgg gcctgatcta
     2641 cgtgaacccg gagggccccg aaggccagcc ggatccactg gccgcggcca aggacatccg
     2701 cgagacgttc agccgcatgg cgatgaacga cgaggagacg gccgcgctga tcgtcggcgg
     2761 ccacacggtc ggcaagaccc acggcgccgg cgatgccagc ctggtcggac ccgagcccga
     2821 ggccgccccg atcgaggagc agggcctggg ctggaagtgc gcgttcgcca gcggcaaggc
     2881 cggcgacacc atcaccagtg gcctcgaggt ggtgtggacg aacaccccga cccagtggga
     2941 caacagcttc ctggagatcc tctacggcta cgaatgggag ctgaccaaga gcccggccgg
     3001 tgcttggcaa ttcatcgcca aggacggggc aggcgcgggc accattcccg acccgttcgg
     3061 cgggccggga cgtgcgccga cgatgctggt caccgacatc acgatgcggg tggaccccat
     3121 ctacgggaag atcacccggc gctggctgga ccatcccgag gagctggagg aggcgttcgc
     3181 gaaggcgtgg tacaagctgc tgcaccgcga catgggaccc gtctcccgct acctgggccc
     3241 gtggatcgcc gagccgcagc tgtggcagga cccggtgccg acggtcgacc acgagctgat
     3301 cgacgagcag gacgtcgcga ccctgaagtc caaggttctc gaatccggcc tgtccgttgc
     3361 ccaattggtc aagaccgcgt ggtcgtcggc ggccagcttc cgcggcaccg acaagcgggg
     3421 cggcgccaac ggggcgcgta ttcgccttga gccgcagaag aactgggagg tcaacgaacc
     3481 cgccgagctg gccaaagtgc tgcctgtcct ggagaagatc cagcaggagt tcaactcgtc
     3541 ggcctccgga ggcaagaagg tctcgctggc cgacctgatc gtgctggccg ggtccgcggc
     3601 ggtcgagaaa gcggccaagg acgccgggtt cgacatcacc gtgccgtttg cgccggggcg
     3661 caccgacgcc tcccaggagc agaccgacat ggagtcgttc gccgtgctcg agccgagggc
     3721 cgacggtttc cgcaactacc tccggccggg tgagaagaca caggtggaac ggctgcttct
     3781 ggagcgggcg tacatgctga acctgaccgc cccggagttg acggtgctca tcggcggcct
     3841 gcgggcgctg ggcgccaact tcggcggcag caagcacggc gtgttcaccg accggccggg
     3901 aacgttgacc aacgacttct tcgtgaatct gctcgacatg ggcacggagt ggaaggtctc
     3961 ggagtcgtcg gagaacgtct acgagggtcg ggaccgcgct tcgggccagg ccaagtggac
     4021 ggctaccgcc aacgacctgg tgttcgggtc gcactcggtg ctgcgcgcgc tggctgaggt
     4081 gtacgccgaa gacgacgcga aagacaagtt cgtgcaggac tttgtctcgg cgtgggtcaa
     4141 ggtgatgaac aacgatcggt tcgacctcgc ctgatcgaaa acctcgttcg acgccccgcg
     4201 agctgacacg tgctcgcggg ggcgtcgtgg gtttagggtc aatcgcgtta tgacgccgtc
     4261 acagttggca gagcgggtcg agcgcgccgc tgaggcggct ttggccgaac agcggtatgt
     4321 cagcgccatc gacgtcctgc tcggtttggg gtggctggcg cactctcacg tcgaccgatg
     4381 gcgacagggc cgaatcgagt atctggacag cgtcatccag gccaagccga ccaaggtcgc
     4441 gtctgcgatg tcggcgttcc atcgctgggc gcaggaccag aaactgaatc cgtcggagac
     4501 tgattacgta gcccgcactc gggaccgccg ccagctgcgg ttcagcgcga ccgaagacgc
     4561 cgccgtcgag cgcatgtacc gcacccactg gctgtcgccg gagttgtctg agggtgcggt
     4621 caagcggcag agcaagccgc ctgatcttgt ggtgatttcg ccactcaaag agtggaagtg
     4681 tgcgtcgtgc ggcggcaccg gtgacctgct gttcatggag gatgccggcc cgctctgcct
     4741 ggactgcgcc gacctcgggc atctcgtgtt cctgccgtcc ggtgacgctg cgctgacccg
     4801 gcgggcgaag aaagccagcc ggctctctgc ggtggtggtg cggtggagcc ggtcgcgtaa
     4861 acgctatgag cggcaaggca ttctggccga ggcagaggcg gtcgagcgtg ccgaacagga
     4921 ctgcttgtcc gatgccgagg tgcgggcgcg ccgccgagaa cgggatgaag cgcgacgggc
     4981 cgacgacgac gtgaagttgc aggccgagtt cgccgccgcg attcgggcgc agtttccggg
     5041 gtgtgccgtc agccgggcgg aggcgatcgc acaccacgcg gcgacgcgcg gcagcggcag
     5101 ggtcggacgc agtgcggcgg gtcgggcgct cgatccggac gccgtgcggc tcgccgtcgc
     5161 agcctctgtc cgccatgtcg acaccgacta cgacgagcta ctgatgtccg gcgtcgaccg
     5221 ggagactgct cgacatcggg tgggccagcg cgtcgaggag gtcttgaggg gctggcgtgc
     5281 tggcgaggca gccacgccct cgccgcgcga ctagtttgat actactacgg tagtaccgta
     5341 gtagtatcac attcatgaag ttgagtgtga gtctttccga cgaagatgtc gcagtgctag
     5401 acgcatacgt tcagagagcc ggattgtcgt cgcgctctgc cggactccag cgtgcgattc
     5461 gcatgctgcg ccaccccact ctcgaagacg actacgacaa cgcatgggct gaatggcggt
     5521 cagccggtga cgaggaagaa tgggagaaca gcttgggcga cgggctcact gatgcagcgc
     5581 ggtgagattt ggcaagtcga tctcgatccc gttcgtggta gcgaagccaa caagcggaga
     5641 cccgctgtca tcgtgagtaa tgaccgcgcc aacgccacgg cgacgcgcct tgggcgtggc
     5701 gtcgtcaccg tcgtacctgt aacgggcact atcgcgaaaa tctacccgtt tcaggtcttc
     5761 ttgcgcgccg cgacgactgg tctcacaacg gactccaagg cacaggccga acagatccgg
     5821 tccgttgcca ccgagcggct gatacgccgg atcggccgca tctctcccgt ggaactcgcc
     5881 gaactcgacg aggcgctgag gctgcatctc gacctgtggt cgccctagcg tcaacccgct
     5941 gttgccacgg cgtggcgact cccccggtat tgtcgtcatg ttgtcacccg aagagtggtc
     6001 aaagcgcggc cgcgcgacgt ggacgtaagg acatgatgta tgcgcgcgct gcatgacccc
     6061 gaaccggtgc ctctgccggg tcctggcgtc gctcccacca gtccgcagcc tttgtcggtc
     6121 ctgttggtcg aggacgaccg ggccgatgcc ttgctagtcg aagaattgat cgccgatgcg
     6181 gcggtcgaca tccgtttggt gtgggcccat tcggtggcgg atgccgagcg cgagatcgcg
     6241 cgcgcccgcc cggattgcgt gctcctggac ctgaacctgc ccgacgcgaa cggcatcgat
     6301 gccctcgacc acatctgcga ctgcgatgcc acgcttgcga tcgtcgtgct gacggggctc
     6361 ttcgacgagc acttcggcgt ctcggcggtg gcctccggtg cccaggatta tctggtcaag
     6421 gggcgtgtcg aacccgacat gctacggcgt tcgctgctct atgcgatcga gcgcaagcgc
     6481 gccgagctga cagccgtcga cctgcacgcc agccagatac gagcccgaga aaacgcgcga
     6541 cttgaacgcg gcctgctgcc ctctcccctg ctgctcgaca accccggcgt cgagattgtc
     6601 gcccagtacc ggcccagccg cgagaacgca ttgctgggcg gcgacttcta cgacttcgta
     6661 caaacgccag accgcaccgt ccacgtcatg atcggcgacg tggccgggca cggccccgac
     6721 gaggcggcgt tgggggtggc gttgagaatc gcctggcgca cactgacatt cgccggtctg
     6781 aggggttctg accggatgcg tcagctagaa cggatactgc acgctgaacg cgccggcaag
     6841 gggatcttcg cgactgtgtt gagcctggct attcctcccg acagctcacg catcaccgcg
     6901 gttcgcgccg gtcaccccgg aatgttgctg cacggtgggg gcaccgtcga atggatcgag
     6961 ccgccgggtg ggccggcact gggcctgcac accggcgact gggcgccaca gcaggtggaa
     7021 tggcccgagg gcaaggggct catcctgctc accgacggcc tcttcgaggg ccatgccggc
     7081 cgcggtaatg aacgcctcgg cgagcagggt ctgcttgagc tggcccgctc actggcgaca
     7141 ctgcccggcc cagagttcgt cgacgcactc atcaacaacg ccgaacaacg cgctcaggcg
     7201 cacggcgggc tgaccgacga cattgcagtg gtccgcgtgg agcggaccac gtgagttccg
     7261 gtacgccagt gcaccaaagc gccgccgggg ccgcgccaca gcgcggatca caactcaccg
     7321 tgcagggatg gcagaacctg gttctggccg tcatgggtgt gttggtgctc gcgggcgcgg
     7381 ttgccggtgc cgtcttgctc aaccgcaccg acgagatgtc cagggagctg atcgacgaca
     7441 ttcagccggc gcgggtagcg gcataccggt tgcaagccgc acttcgtgac caagagacgg
     7501 ccgtccgcgg ttacgtgctt gccgccgacc ggcagttcct cgacccgtac tacgacgggc
     7561 aacgcgctga acaagcagct gccgacgaca tccggcagct tgtgggtgac cgctccgagc
     7621 tgatgaccga tcttgacgtg atagagcggg cagcagccga gtggcggacg aattatgccg
     7681 agccgctcat cgcgatcgtg acccgcggca ctattaacgt cccgaaaacg gatctcgtcg
     7741 atcgcggcaa ggcagaattc gaccggctgc gaacgctttt cgacgctcag aacgagcatt
     7801 tggcctcggt gcggacggcc ggcatcaacg acctcgacga gatacgggca tggcgcgaca
     7861 ggttgctggc cggtttggtg gccactttcc tcgtcacggc gatcgcgctt gcggtcctgg
     7921 tgcgaagcgc ggtgacccgt ccacttggtg tactcgcgac ggcatgtcgg cggatcgccg
     7981 cgggcaactt cggcgaaaag atcaccccgc gcgggccgaa agatattagg agcattgcga
     8041 aggacgtcga gaacatgcgg cagcgaatcg tcgaggaact cgacacatcc cgagcggctc
     8101 gagaggcgct ggccgagcag gccgtggagt tgcgacgttc caacgccgaa ctggagcagt
     8161 tcgcctacgt cgcctcccac gatttgcagg aaccgctgag aaaggtcgcg tcgttctgcc
     8221 aactgctcga gaagcgttac ggcgacaaac tggaccagcg cggtgcggag tacatcggat
     8281 tcgccgtcga cggcgccaaa cgcatgcagg tgctcatcaa cgatcttctc acgttctccc
     8341 gggttgggcg cctcaacgcg tcggagacag aggtcgacct ggattccgcc ctggatgccg
     8401 cactgggcaa tctgaccacc acgatcgagg agacaggtgc caagatcgtt cgccaaccgg
     8461 ggcagctccc acacgtcctc ggcgatccga ctctgctggc gatgctgtgg cagaacctga
     8521 tcggtaatgc ggtcaagttc cgccgcgacg gtgtcgcacc gaggatcgtc atcgattgcg
     8581 agcaacgcga cgacgaatgg ctgctcagcg tgtcggacaa cggcatcggc atcgcagagg
     8641 agttcgtcga caaggtcttc gtgatcttcc agcggctgca tggccgggat gcgtacgccg
     8701 ggaccggcat tggtcttgcc ctgtgcaaga agatcgtcga gcaccacggc ggcacaatct
     8761 ggatcgatac gtcctacacc gacggaacaa ggttccgttt caccttgccc cccatgcccg
     8821 caaccgtgga cgaaacagcc gcggccactc tggaaggaac acatctatga caccggccgg
     8881 tcgtccgatc gacgtcctgc tcatcgagga cgatccaggt gacgtgctca tcacgcggga
     8941 agcgttcgag cacaacaaaa tcaacaacac cctgcatgtc gcgcacgacg gcgaggaggg
     9001 tctggacttc ttgtatcggc gtggtgcgtt tcaggacgtg ccgcggcccg atctgatcct
     9061 gctcgacttg aacctgccga agtacgacgg ccggcaactg ctggagaaga tcaagtccga
     9121 tcccgagctc agtcacatcc cagtcgtagt gctcactacc tcttcggcgg aggaggacat
     9181 cctgcgcagt tacaaactgc acgccaacgc ctacgtcacc aaaccggtcg acctcgacca
     9241 attcatgaat gctgtgcggc agatcgacga gttcttcgtt caggtcgtcc gccttccgca
     9301 acccggatcc gctgggggtg cttaggcggg cttgccaaag ttttttctta gcaaccagtt
     9361 aggtgctact tccggtcacc ttagttttgg ggattagcgt tcaaggaaat tgagtgggcc
     9421 gtgcaaccgg cccatcgcta ccaccccatc gagaatgagt ctgctgatga ccgccacgct
     9481 tactacgccg gctgcccgat tcgacaatgc cgcgttcgac tgcggtcctg ctcaaatccg
     9541 ggctcacttc cgtcatctgg caacggtggt gacgatcacg ggccgcatcg acgccgccaa
     9601 cgttgacccc gtcaccgagt acactcggcg gttcatcctc gcgaaggacg ctctgattct
     9661 cgacctgagc gatgtgaacc ttcctgcggc agaggccatc tcattcgtga acaccgtcgc
     9721 cgcggagtgt cgcgcggccg gggtggagtg catgctggtg gctggcgaag cggtggttga
     9781 cgtactggac gacttcgacg acgaagccct gcttccgatt gccggctccg tccacgaggc
     9841 gctgcactac ttcgccgacg tgattgccac gcgccgccag ctgctgctgc cactgatcaa
     9901 gaagactgcc taacggccct gcagaatgaa gtcggcggcc cgccatgcca tcgccatgac
     9961 tggcccattg aggttgccgg cgaccatcgt cggcatcacc gaacagtcca cgacgcggag
    10021 attgtccact ccgcgcactc tcaactcgct gtcgacgacg tcatcatcgt tggggcccat
    10081 cgcacagcta ccgacagcgt ggtagccgca gtagccgcca tccagcgcgg agtcgacgag
    10141 ctcgtcgtcg ctctgggcat cgggtccggg gaaagtctcg tgcctgatac ggccggcaat
    10201 cggtgactgt tcgaagacct ttcgcatctt ccgcagcagg ttcgcgccgg tctttcggtc
    10261 gtaatcgctg gcaaagtagt tcggttcgac ccgaaggacg gcagtcgggt cggcggaggt
    10321 gatcgtgacg cttccctcgg acgttgggcg caacacgaaa cccaaacagg aaataccggg
    10381 ggcgcgctcg acggcgacgg gctcgcccac attgtaggcg gggatcgtga acggccccat
    10441 cagcagctgg ccgtcgacac gctcggcgtc gggggtggtc ttcacgaacg ccagcacgtc
    10501 gaacgacgga gcggccagcg gacctctgcg ggaggccacg tatcgtgctg cggtgagcgc
    10561 ttgaccgaag ctggtggcca gttgtctgtt gtagccgagg tcctcggaca gcctgaatct
    10621 caatgtcata cagcgatgtt cacgcattcg tcggcccaca ttgtctcggc cgagatacac
    10681 cggtacaccg gcgcgttgca gcacgtctcg tggtccaatt cccgacagct gcagtacttg
    10741 tgggctgttc aggctcccta gcgcgacgat cacttcgcga atcgcccgca ggtccgccgt
    10801 gccatggcgg ccagcgacct cgaccccgac ggctcggcca tgctcgaaca tgatccgacg
    10861 gacggtggtg tgagtgatga cggtgaggtt aggtcggtgc cggatgggct tgaggaacgc
    10921 gtcagcggcg ctgacccggc ggccgtttct gatcgtcgac gtggcgtacc ccacccgctc
    10981 ggtgtcggac tcgttgatgt cttgaacttc ggtgagcccg gttttcgttg cagcttcgac
    11041 catttcggcg cacagcgggt cgggatcgcg gggcaccgtg acgggcagtg gccccccgac
    11101 accgcgcgtc ggtgatgcgc cgaacccgtt gttttcgaat gccttgaaga tcggcaggat
    11161 ctcatcccag ccccagccct tgttgccgag gtgctccaac tcgtcatagt cggcgcggtt
    11221 accgcggttg tagaccaggc cgttgatgga gctcgatccg ccgagcacct tgccgcgcgt
    11281 ccagaactcg gaccgctggt tgggcccgaa cggtgtggtg gcgtagcgcc acacatagtt
    11341 ctcgttctcg aaaatctttc cgtagccctt gggaatgtgg aagtacggac tgcggttcgg
    11401 gccacccgct tcgattacga ggacggtgat gcgcgggtcg gcggacaacc ggttcgcgag
    11461 cacacatccg gccgatccgg ctccggcgat gatgtagtcg tacctggtca tgggatgcct
    11521 ttgagcagtg aagatcgggg tccgggcggg accagcggat gttatcgctt ggctgggcgg
    11581 tacgctacgg attcgtgagg gcagacagat cagcggtggt gatcgccacg tttgtccttg
    11641 ccgcgtgcgc attgtccgtc ggcgtcgcca gcgccgatcc gcccgcccct gcacccgaac
    11701 ccaagacgac gatcgaccac gacggcacct ttgtggtggg aaccgacatc gtgccgggca
    11761 cgtacacctc agcgggaccg gttggcagcg gtacgtgcta ttggaagcgg atgagcaatc
    11821 cggacggcgg tgacatcatt gacaacgcgc tgagcaagaa gccgcagatc gttcagattc
    11881 tgccgagcga cggtgcgttc aagaccaacg gctgccagcc ttggcaaatc acggatgcgg
    11941 tccctccagc gggtgtgcct ccgttgatcg cgggcgcgca actgcaaggc atcatcaacg
    12001 acatcaatgc gcgcgcaggt cagtccggcg cagggtaagc cgtcaccgaa gcagaagcca
    12061 tggtcgtgtt tcgccgccga tcacagccac atcttcggtt tcgacgtgca acaggtcgca
    12121 cgcacagcca tgggaccgct ggcccacttg gtggccgatc atggcctcgg tgtggtggtg
    12181 cccgaccggg ccgccgacgt ggcggcccgt atcgtcgcgc tcaatagtcg atgacggcgg
    12241 gctctttgcg ttcggtgatc ggccgcgcga gtccctcctc gtcgcagact cgctgaaggc
    12301 gctccagagt cacgtccaga ccgggtccca gcttgggtcc cggtgtgaac gtcgcgggca
    12361 agtgcttcat cccttggatg acaccgatgg tgtcgtagtg cacggtacct tcggggtcac
    12421 atcggtagtc cggcatccgg tcgaggacag cggtcagcat ccgtttgaaa acgattcgcg
    12481 ccacattgga tccgatgcac cggtggatgc caagcccgaa gctgaagtgg cgattggcgc
    12541 cgcgctccaa aaccacggtg ttgggatctt cgaagaggct gggatcgcgg ttggccatcg
    12601 cccacgaaac ccacagccgc tcaccttgtt tcaatctggt gccgttgatt tcgcagtctt
    12661 gggcgatcgt gcgaccgtcg cctggtgccg gcgtgaagaa ccggaggaac tcctcggtcg
    12721 ccgagtcgag gagctcgtcg cgatctcgac tcagccgcgc gcgttcgtcg ggatgttctg
    12781 aaagccattc cagcgcatgg gcggtcaatg cggtggtggt atcgaagccg ccgccgatca
    12841 gcagagtgag cattccgagc agttccatct cgggtgccgg ttcaccgttg atcctcaact
    12901 tcaccagcgc gtcgacgagg ccgggccggg gcacctcgcg gacctcggcc aggttacgca
    12961 gcagatcggc catcatcacg gtgtacattt cggccacccg cgacgtgtcg ggcgagtccg
    13021 ctggtgtgta cacctgcgca tgagctggtt cgttgtagat ctgccaactc gccagcggaa
    13081 tccccatcag ggcaagggtc aacaccgccg ggacgatgtt ggcgaggtcg tcgacgaagt
    13141 cgatgcggcc ctcttcgatc ttctcgtcga gacaggcctt gacgatttcg tcgacgaccg
    13201 gtgtccagcg ctgcaccgcc gcgggtgaca agtacgggtt gagcaccgtg cggtagagtc
    13261 ggtgctcggg ttcgtccatc tccagcatgc cgccgcgtac gtgtgccgag tactcctgct
    13321 gcggaatctg aatgcctttg tagcctttgc gttcaccgtt gacatcgttg tcattcgaga
    13381 ggtgggggcc gcgagcgagt tcgaacacct gctcgctgcc ggcggcgacc cagtgcccgc
    13441 cataggtgtc ggtccacgcg agcgggcacc ggctctgcat ctgctcggtg accgtctgga
    13501 attgttcgcg gtattccggc gtgtgccgat cgaagtggta gcgggggtcg tcgctatggg
    13561 cgtccagagt ggcttcgttc tgcatcgcgg cttcctcgga gtgagcgtcg tcgatctcaa
    13621 gtgtcaccta tcagacgccc gtaccgtgaa gttatgacgg tgctgcctga cttgaacaca
    13681 gttcgtcaga cgctgggtcg aagcctgttc ggcatggtgg ccgggcccga cggtccagcc
    13741 aaccgcagcc tcatccacca cacaccgggc ccgcgatggt tcgccgagga tcgtccgatc
    13801 cgccgggtgc atgcggacgc ctcaatgttc gtcggtgggc tccgggcact gctgctgcag
    13861 tcgttgcatc cgttggccat ggccggcgtc gcggaccatt ccgactaccg gggcgacccg
    13921 tggggtcgac tccagcggac cagcaccttc ctggcggtca cgacgttcgg cccggccgcc
    13981 gatgcgcagc gcgcggtcga ccggattcgc ggaatccatc agcgggtccg cggaaccgct
    14041 ccggacggca gcgcatacca cgccgccgac ccgcacttgc tggaatgggt gcacgtcgcc
    14101 gaggtcgaca gcttcttgct tgcccaccgg ctctacggag cgagcccgct ggaccagagt
    14161 ggtcgcgacg gatacgtggc cgacaccgcc cgcgtcgcgc tggctctcgg cgtacccgac
    14221 ccgccgcgaa gcgagcgcga actgcacgaa cgcatccagg actttcgccc cgagttgcgc
    14281 ggcaccgctg ccgcacggga tgccgcccga tttctgttat tcactccccc gctgccgatc
    14341 gcggcgaggg ttccgtacgg ggtgctggcc gctacgtcgg tggcgatgct gccggcatgg
    14401 gcgcgaatgc cgttgctgct gccctacttt ccgcctgtcg aggccactgt gatccggatg
    14461 gcggggcgcg cggtggtcgg tggcatccgc tgggccatga cgggatgagc acgcgctggg
    14521 ggcgagtcgg cgcaccccgc ggtgacgact acgacgagag atggagacac ctggccgcgg
    14581 tcggccacaa cgtgcacggc gaagcggatt tggtcgagca attgcttcgt gagaccggcg
    14641 gaacccgtgt gctcgacgcg ggctgcggca cggggcgggt ggccatcgag ctggcccggc
    14701 gcggtttttc cgtcgttggt gtggatgctg acggcggtat gctcgacgcg gcccgcgcca
    14761 aggctccaga tctgacgtgg cttcacacag atcttgccga cttttcctcg agtgccggta
    14821 ctttcgacct ggtggtcctg gcgggcaacg tgatgatctt cctcgccccg cacaccgaag
    14881 ggctggtgct gcaggccgtt acggccagcc tcgtaccggg gggccttctc gtggccggat
    14941 tcgcgattcg cccgcatcgc ctgcagctgg cggagtacga ccggttcgcg caggcggctg
    15001 gtctggtgcc tgtggcgcag tgggcgacct gggaccgtga gccgttcact ggaggcgatt
    15061 acgcggtttc cgtgcacaag cgcccggcga tctagcaggc tcgcaggatg aagctgccgg
    15121 aaccggattc gggcatccca cacctggccg acgtggttcc gtcggtgctg gcggcgatgg
    15181 gcgtcgcgag cttagaagcc cgaatcgggt tgcctgcaga catatctggc gcgtgcgtgc
    15241 tactgatcga cgggcttggc gccgaactgc tggacaccta cgcggcggac gcgcccgtgc
    15301 tggccgagat gcgggggcaa acgctgcagg tcgggtttcc gtccaccacc gttgccggac
    15361 tcgccgcgct cggcaccggt tgtcgttccg gcgagcacgg catggtcggc tacagcttcc
    15421 ggctgccggg cgccgggttg ttcaacgccc tgcggtggcg gccgcacccg tggggcgagg
    15481 acctgcgcga cgacgtcgcg cccgagcagg tccagcccat gccgacaacg ttcgaacggg
    15541 ctgcgtccgc cggagtcgcg gtgagcgtgg tctccggcgc ggagttcacc ggctccggcc
    15601 tgacccgggc ggtgctgcgc ggcggacgct acgtcggcgt gcatgccctc ggagatctgg
    15661 cggtgggggt acgggccgcc atcgccgacg gcggcttctg ttacggctac cacggcgagc
    15721 tcgatctcct cgggcacgtt tacggtcccg gcgcggcggc atggcggtcg cagctgcgtc
    15781 aggtcgaccg gctggtcgag tccgtggtcg agagcctgcc gcccggcggg ctgctcgcgg
    15841 tcgtggccga ccacggcatg gtgtccgtgg acagcgcaaa cgctgtcgac gtcgacgact
    15901 gcgagcagct gctggacggc gtgcaggcga ttggcgggga gccgcgagcc cggcacgtct
    15961 atgtcgcgga cggcgctgct gaggcggtgc tcgcggcgtg gcgggaggca ctggcggacc
    16021 gggcgtgggt ggtgtcgcgg gaggaagcca tcgcagccgg gtggttcggt gagcgtgtca
    16081 ccgacgacgt acgtccgcgc atcggcgacg tcgtggtggc ggcccggggc tcggcggccg
    16141 tggtgcgtcg aaaagtggag ccgctggagt cgtcgctgat cggacaccac ggctcgctga
    16201 ccaccgccga gcagcgagtg ccgttgctgc tggcctacgg ctgaggctca gcggctcgtc
    16261 gagactgcgt atttgcagga gaacttcgag tgcgggcctg ccggaatctc ccccgcaagc
    16321 gggaggtgcc cccagtttcg gcgcagccct agagaacgaa cgacgaggag atgacgtctc
    16381 cgcggggcaa ctgctccctt gtcgagcttc gagaacaaca cgccgatgtt gtcgccggct
    16441 gccgcctcac tgatcgtctt gcggaaggtc tcgatggcgt cgaccgtgac actgggaccg
    16501 ccgttgatct gcacctcgtc tccgacgcgg agttgcccat gctcgacgcg acatgtcgcg
    16561 acgatgccgc tcgattacgg atgacgaaca cgtcgtcgac tgtcatgcgg aacatgtgtg
    16621 gcaacattac tcgccgggtc gctctccgtt gaaaaggctt gcagtacaac gtcaattcag
    16681 accgcgacgg gcgaaatcag cgaggaactg gtaagtccgg tcatacgcgt ccgaatcact
    16741 ggccagtgca atgcgcgatg ccacgaagtc ggtgacacca atctgatgca gtcgttccag
    16801 ttgtcggcgg acctgtgtct cctcaccggt tatcgcgaga tcgcccatgg tttggaggcc
    16861 ttcgtggttg agcagtgcac ggtacgacgg ccacctttcg tagcccgggt acatcgcctg
    16921 ggctgccagc gcgcgccgtc gcaatctttg gtgacggcta ttgggaataa cgccgccact
    16981 cgtggggcac ttcggcctgc cgcgtgcgcg ggcttcgtga tgcgaggtag cacaattgtc
    17041 tcgatgtaac ggtgcccggc cgcgccgcgc gcggtgtcat ctgcaatttc gccggcgagt
    17101 tgcagcatct gcggctgcaa cgcggcgact aacacagacg gcggcgagac acctgacggc
    17161 acatccgact tcagcttcac tcgataatcg tggccggtca cgtccaccga ctgatcggcg
    17221 agcaatggca tcagcacctg caggtactca cgcagatggc ggatcgggtg cgaataatcg
    17281 aggccgaagc cggtttcgat aaccggcttg tggctgagcc cgatgccaag cgtgaacctg
    17341 ttcgcgctga gctgctggat ggtgagtgcc tgctgcgcaa gcgtcgccgg atgtcgcgga
    17401 aaggtggcac cacataactt cccagctcga ttcgcgacgt cattcggctg agcgctccga
    17461 gtaagaccag tgcatcaggc ggaccgaccg acggaaggct cggcgcccag actgaactga
    17521 agccgtcctc ttccacctgc ttcaccacgc cgaacaattc ctcgactgga cgcggaatgt
    17581 cgaatggtgg cgtgaggccc aggccggtac gcatcaagtc atccgatttc tagtctcacg
    17641 cgaccggact agaacctcct cgattgccgt cagcgtcacg ggatcgacga tgcatgtgaa
    17701 gtggttgctt tccggtgtct cgaccacccg taccgccggg ttcgtcgcca acagggccga
    17761 aacggcgcgg tcagggacga ttaaccctcc gttcagcggc ctcatcgccc tgacgagcag
    17821 ggtcggcatg gtcagcgccc gccaatggtc gctccagtcg cgcggccact cttgatagag
    17881 gtcttcctcg tcggcctcgc gcgacgtcga tcggaaccac gagccgtcgt cgagctgctc
    17941 gagttcgtac gtcggcgtgg tcgatcaccc gccggcgcgg atggcctgaa gatagaggtt
    18001 cgcagacaga ccgtgaacgc acagcaacag cggcgctcct tcggcgcccc accggcgggc
    18061 acgtacacgc ccggaaggca gactcaggtc gaagtccacc cggcggactt tacgcgtgtg
    18121 gcggctatgc gcccgctcgt actcgctcga cgatcgtcac gatctcgtcg gcaaccagct
    18181 gcggttggta aagcatcacg ttgtggccgc tgtcagtggt ggtcaggttg acggtgccga
    18241 gcgcgtccgc cagcagcgtg ttggcctgct cgatctgggc cagcgtgtag ttgtcgggct
    18301 tgagctgtgc gggcggcggg aacttgtcgg agccgagcac gaccgccggc acctgcggca
    18361 aaggaggcgc ggcagctatc cgtgcgtacg cgtcgtcggc gaggaatcct tcaccgccaa
    18421 cgtcttccgg caccgccgcg tcgtggttga acgtggcgtt ctgctgcgga ctccccaggg
    18481 tcggcaggaa ttccgacacc gggtcgacca gcacgagacc gctcaccagt tccggatggg
    18541 tgcgagccaa caggtcggcg acgagcccgc cgtaggaatg ggccgccacc accacgggcg
    18601 tcgaaatatc agcggcagca attagtttca cgatgtcgtc aacgtcctgc gccacggtgt
    18661 gcggctgcgg cacgggggtg gattgattca tcccgtcggg tcgggtgtcg ggacggtcat
    18721 aggcgcagac gcgggttgtc cgtgccacgg ttggctgtgt agcggtagga ctcggttcga
    18781 gttcggcctg ctcgataagg tcgtacggcg acgaccggat cggatcatgt tcgggcacaa
    18841 cgacattcca cacgtcggca tagctgccct tgcctgggat gatcaacacg gtcggcgatc
    18901 cgtcgccctg gcaatccagg tagatacttc gtccgccgcc gatgtcgacc aagccggcat
    18961 cggcggttgc ccgtccgcac ggcagcaccg tcgcggccgc tgccacaagt gcgacgacgg
    19021 ccggccggta caacgctgcc atacctgacg gtaacgtgtg gcacatgagc gctgcgccga
    19081 ttcaagacat tcctctcaag acgctcgacg gcgacccaat ctcgttgtcg cagttagccg
    19141 agtcggtgac ctcattcgtg ttgatcagca atacggtgta atcgtcgggt tgcgctgtgg
    19201 cagagccgat tccaaatgcg gccgcggcaa cggtgagtgc tgccgtcgca gttaggggtg
    19261 ccggcccaga ccgcggggtc acgacccccg cggcagaggc gtgatcagtc gtctgacggg
    19321 atcactactg gggttggctc tcgggacatc acgtggggcc gaacaccgtc tacggagtgg
    19381 gtgggaaaga tctcgacgtc tacagcttct acgcgaagga ccccaaggtg ccctggccgt
    19441 accgcaggca cggcaaggcg gacttcgggg agtcggagtt cgggtactac cgccccgcgg
    19501 gccgccccta cgagtacaag ggcgagcggt tcgtcggccg gcccgtggac ctactggggc
    19561 gggcgctacc ggtggagccc gacgccgacc cggtgcaggc cgttcgggac tggctggcca
    19621 cgagcaacaa cgcgacgccg cggctcctgc ggttgaaggc ggtcgtaggg ctatacccgg
    19681 caaatatcgc ggcaaggtcg agataccgat ctaacggcgg gcgaaaagcc gcggctctca
    19741 ctcggcgctg atgacggccg gctccttgcg ttcggtgatc ggtcgggcga gcccctgctc
    19801 atcacaaatg cgctgcagcc gctccaacgt gtcgtcgagg cctgcgccga gtcggtggcc
    19861 gggcgtgaag gtggccggta ggtgctgcat accctggatg accccgatgg tctcgtagtg
    19921 cactgtgcct tcgggatcac accggtagtc gggcatccgg tcgagcaccg cggtcagcat
    19981 ggacttgaag acgatccgcg cgaggttgga cccgatacaa cggtgcacac ccaggccgaa
    20041 gctgaagtgc cggttacctt tccggtcgag gacgatttcg ttggggttgg cgaacaccga
    20101 cgggtcgcgg ttggccatcg ccccaggaca gccacagccg ctcgccctct ttcaagtgca
    20161 cgccatcgat ctccgtgtct tcggcgatgg tccgtccgtc acccggcgcg ggggtgtaga
    20221 accgcaggaa ctcttcggtc gccgggccca gcaggtcgtc ccgctcacgg ctcaaccggt
    20281 cgcgttcgtc cgggtggttc gacagccact cgagcgaatg cgccgtgaga gcggtcgtgg
    20341 tatcgaagcc gccgccgatg accaggctga gcatgccgat gagttcgatg tcggaaggtg
    20401 gctcaccgtc gatgcggatc cggttgagcg cgtcgatgat gcctggtcgc ggatgctcac
    20461 gcatctcgat gagattggtg agcaggtcga cagtcatcgc cagagagagt tcccgcacgc
    20521 gctcgatgtc gggggagttc ggcggcgtgt acacggaggc atgcgtcggt tcgttgtaga
    20581 tggcccactt tttcagcggg atacccagca tggcgagcgt gagcacggcc gggacgatgt
    20641 tggccaggtc gtcgacgaag tcgatccggc ctgcctcgat cttctcgtcg aggcagccgc
    20701 gaaccgcctc gtcgatgaag ggctcccaac gcgcaaccgc cgcgggggac agataagggt
    20761 tcagcgcgtt gcggtagatc cggtgttcgg gatcgtccat ctccagcatg ccgccccgga
    20821 aattctccac ttcgacgatg agcgggatgg agatgccttt gtagccatgg cgcacaccgt
    20881 gcacatcctt gtcattggaa atacctgggc agcgcgcgag ctcgaagacc gcgtgactac
    20941 cgccggcgac ccaatgaccg tcatacgtgt ccgtccacgc gatcgggcac ctggcgagca
    21001 tctcctcggt gatcgcctcg aaccgttcgc ggtattcggc ggtgtgccgg tcgaaatggt
    21061 agcggggcac ccggagacgg ccgccctcca cggcatcctc ggtggtcacg gcgtcgctcc
    21121 ttcccagtag ctatcctggg gtcgcccacc ggcgagacct gacaccgatc acgatagccc
    21181 gctctggaga ggaccgggcg accttgcgaa cctcgccctg ggatcgggcg ggacgtcatt
    21241 gctgacggcg agctgggcag agtgagggtg ccaccgcaat ggcgacctac gcatacgacc
    21301 agatcgacca ggcccgaaca gaactgaacg ctgtctcgaa atagaccaca tctgcgacac
    21361 ccgcacacct gagggagcgg gcgcccgaat gtcaaggtcg cggtgtcttg agggtctgcc
    21421 ccgaaaatgg ctgtctcatc ccggtacgtt gagcagttat gagaggggtc accaaggaga
    21481 gcgggcgtca gccggctggc gggggcgcat cttgatggac taagcccgcg ccggcgacac
    21541 cgtggtcgtg tggaagctcg accgactcgg gcgcaacacc ctgaacatcc tggagaccgt
    21601 taaggcgctg accgaccagg gcgtgacgct gtgtcgacca ccgacggcat cgactcctaa
    21661 tcgcgctgcc gcctctccgc gacgagcgag ttgagttacc aagcttggct tttggcagct
    21721 caggcacggt ttggtccgtg cgcaagcccg tcgccgtatg tcgccaggca taccgtaagc
    21781 acttcgcagc gatagccact ctgattgacg acgatccgaa gcacgtcgtc tgcctcgact
    21841 cgttgccctc gcatgggcta agggcggcgc aaagcagtgg agcaatggtt ctcagtgccc
    21901 cgttaatgca gccccatatg gtcatgtgcg acaacttgat tgagcgatcg cgtactacgc
    21961 tgcgcagtat tgttgccgca ctgcggttca caaactcctg gtgcgacagg tcgactgagc
    22021 aaggacaggt gtcctcgtat tgctgttggt tgcttcgcga gacctgctga tgggcgctga
    22081 ctttggctag tcgctcacgc gaggcctagg tacgacgcag cgaccgtcac tgcctcgtcg
    22141 tcgacggcta gcgtgcggac gccgcgcacg cttaatccac ccagggcgcg gtcgaacagc
    22201 gtcacctgca tccagctcgc ctccagaatc gaccaccacc ggagcccggc cgcgtcagtg
    22261 tggcgctcga actggccgac cgcgtaggcc tgtgtgactg agcgctgacc ggtcgccacg
    22321 atggacggac gcagcgactc tgtgaccaac accgcagggt catcgaggtc gatgagtgtt
    22381 gcgtcccccg caagctccaa ctcagccagt gcgagctgtc gacccgcgcg aacgagcaac
    22441 tcagggtgca ggtccccggt gccccgaaac ggtgcgaggg tctcggcgac tgccgaaacg
    22501 gccatctcag ccaagtagag acagccgtac cgatccggag cgtcgtggcg cccgctgccc
    22561 tgatactccc ggggcaccca gacggcgtgg ccgggctgtc cgaccgtcgc tgacggttcc
    22621 catggcagga cccgccacag cctcacgcga agctgccggc tcgctcgttc gcgatcgcgt
    22681 ccagtacctc ccgtgcgtga ccgcggcgaa tgaggtccac gggtcgccga cctcccaagc
    22741 tcgggttcat accgaccagc cagcgctcgg cggcctcagc cgaataaacg cgtaacaagt
    22801 aagccatcac gagctccagc aggtccacgc gtgcagcgtt gacatcatcg atcccctgac
    22861 ctcggcgcca ccgcgtgacc tgggcggcat tgacaccgag cagctcagcc aggcgccgct
    22921 gggagccgaa gtcacgactc agcgcgatta ccttctcttt cgttgacaca ggcacgaccc
    22981 ccagcctacg caacataata tgttgcgtag gttctgccag gctcatagaa ccggtcactc
    23041 ggtccccgca gccttgcctg ggcatctcaa tgccctctgg cgccactccc gcgctgggga
    23101 atacgacgga cacgaataca gcgggcaccg ttactggttt tgcaccaggt gaatcactac
    23161 cccttcctcc cggggcgact cggcccggcg tcccctccgt accgtggaat gtctgttgcc
    23221 ttgaggcttt cgacctaaag gacgcgtgaa gcggaccctg ctaccgcagt ggctgggtgc
    23281 acttgaaatg tcgtattcgt ggctggctaa gagtgaggcc atggctcacc acggcgtcac
    23341 acaccagcgg ccattccgcc ggtcacccgc agtgtctcac cagtgatgaa gcgcgcgcga
    23401 tctgatgtca ggaacagcat ggcttcgacg acgttggtct ccgtgccatc tgcgtcgatg
    23461 aattgcatcg ccttgatctg cgccttggtg gcttcgggca gctccgcgcg gattgtctcc
    23521 gtcaggatca tgccgggcga tattgcgttg acgcggatcc cgtcggtgcc gagttcgcgt
    23581 gcgaaggtga tggttaggcc ggcgaccgcg agttttgaag tgccataggc ggttccgccc
    23641 atgtgcgccg cggcggatgc aatgttgacg atgttcgcgc cgtctcgccc agccatgtac
    23701 ggttgggcgg ccagcgtaca ggagatcacg ccgagaacgt tgacctcgaa gagtcgccga
    23761 gtcttggcca aacccatctc ccggatcggc ctgctgtatt catcgctgtg caggccagcg
    23821 ttgttgatca agacgtcgat gccgctgtgg gtgtcggcga cctccgtcat gatcgccgcc
    23881 acccggccct catcggtgac gtcgccggcg tagccggtcg ccgctccgcc cgatattcgg
    23941 atgtgcgcag ccgccgattc caccgccgcc ccatcgagat cgataagcac gacgtgcgcg
    24001 ccctcagccg caagagcggc tccgaaagcc ttgccgaagc cgcgcgcgcc tccggtgatg
    24061 accgccgtcg tgcctgccct cgtcctagct ggcgtgatcg tctgatccat ttgtgctctc
    24121 tctcttctgc atccgcctat cgactctctg gccggcgaaa ctaacccggt gcattatgct
    24181 cgtgggtaga ggcttccggt ggcctcgtcc gcgggggcgt tctgaaaaca gcgattatga
    24241 catgcgacgc gaagtttctt gtgccacagg tcctttcacg tacggatagt cctctacttc
    24301 ttaatctccg gccagacttg cgaatatagg gtattgcgcg tcgattcgaa tcgagtgagt
    24361 ggccagcaag cgccggaacc tacatgacgg ggtgcgtagc gccgcgtagc gtctcccttg
    24421 cctgcgacac tgtggtctgt tctagggtct gggcggcttg tcgcggttag tcgctttcac
    24481 ttgactccac ggcttgcagg cgcggccggc ggatcagctg gtcgttatcg tcggtgtcga
    24541 cgccctgcaa gttgatcgct tcttgcgcgc tgctgaaacg ggcgggccgc tgccacaagt
    24601 gcgacaacgg ccggccggta caacgccgcc atacctgacg gtaacgtgtg gcacatgagc
    24661 gctgcgccga ttcaagacat tcctctcacg actctcgatg gcaacccaat ctcgctgtcg
    24721 cagttcgccg atcgggcggt actcgtcgtg aacgtcgcct ccaagtgcgg cctgaccccc
    24781 cagtacaccg cgctgcagaa gttggccggc gactacgtcg accgcgggct gacggtgatc
    24841 ggcgtgccct gcaaccagtt catgggccag gaaccgggca gcgccgaaga gatccagacc
    24901 ttctgttcga cgacgtacgg ggtgaccttc ccgctgttgg agaagacgga cgtcaacggc
    24961 gagggacggc acccgctgta cgaggagctg acgaaaacac cggacagcga cggcgccgcc
    25021 ggtgacattc agtggaactt cgagaagttc ctgctcgccc ccggcgggcg cgtcgtcaac
    25081 cgattccggc cgcgcaccca gcccgacgcc cccgaggtcg tcgccgcgat cgaggccgtg
    25141 ctgcctggat aggcgcccga cctgtcatgc gacactctcg ggatggttgt cgcaattgcc
    25201 cgccccaagc tcgaaggcaa cgtcgcggtc ggtgagaacc ggcagatcgg gtttgccgag
    25261 ttcggcgctc cgcaaggacg cgcgatcttc tggctgcacg ggacccccgg cgcgcgccgc
    25321 cagattccga ccgaggctcg cgtcttcgcc gagcaggaga acatccggct gatcggcctc
    25381 gacagacccg ggatcggttc gtcaaccccg taccagtacg agaccgtgag catgttcgcc
    25441 gaggacatgc gcactgtcgc ggacacgctc ggaatcgaca agatggcggt cgtcggcctg
    25501 tccggcggtg gtccgtacac gctcgcgtgt gcggcgaaga tgcccgagcg cgtcgtggtc
    25561 gccggtgtcg tcggcggcgt cgcgcccgcg tgcggccccg accggatcgg tggcggcctg
    25621 atggcgctgg ggtctcgcat cgcgccgctg ctcgaggtcg ccggcgcgcc gatccggctg
    25681 acggccctga cgttggtccg gctcattcgt ccggtggccg gccccgctgc cggtctgtac
    25741 ggcctggtgt ctccgccggg tgaccggcgg ctactggccc gccccgaaat caaggcaatg
    25801 ttcctcgacg acctgctcaa cggcagccgc aagcagctcg ctgcgccgtt ctacgacgtc
    25861 gtcgccttcg cccgcgactg gggattccga ctcgacgagg tgaaagtgcc cgtgcgttgg
    25921 tggcacggcg acgccgacca catcatcccg ttctcccatg ggcaacatgt agtcggcagg
    25981 ctgcccgacg ccgagttcta ccccatgccg ggggaaagcc atctcggtgg gctgggccgc
    26041 gccgaggaga tcctgcgcac gatgatcgaa acctgggaca aagacgcgct ccggccgtga
    26101 ccatgcgtcc ccgccggact ccgctacgcc cctaacgcgc tctggatcac cgcatcctgc
    26161 ttcgggccga tctcagtcac cagctcggcc ggtgcgggat ccgtgggcgg cccagcgaac
    26221 tcgatcgcag aggccgtgtt gccctgcgtg aaaagcagca cactgatgga cttggacccg
    26281 tccgccgacg tccccgacag cagcgttcca ccggtcccca cttgcacagg ctgcgtcgtc
    26341 tcattggcga cttgaccggc cgcgtccgcc tgagcggcgt tcaacgccgc cgtcgccgcg
    26401 gccgcatcct gcagaaccca cacggtttcg gtgatttgcc gtgacccgtc gcgatgggtg
    26461 tagaccgcct cgacgccggg ctggttgttc ggattgaaaa cgggcgcacc ggctgtatac
    26521 gcggtcgaat cggtgaccgc attggggtcg atcaacagca cggtgtagtc atcgggttgc
    26581 gctgtggcag agccgattcc aaaggcagcc gcagctacgg tgagtgctgc cgttgcggtc
    26641 aaggtgccgg tcgagacgat ggaatttctc atatagtgtc cttcgatctg agacgccgta
    26701 gaagttcgtc aactggtcat cactgatgtg cgctcagcag ggatagcaac cccaaccgtg
    26761 ccaaccatcg cgccagaacc acccgggccc gcagcccctg ttgccggtcg tgcccccaca
    26821 gtcgaaaagt tgcactcccg gtggcggcgt tggggccggc gtgtagacgg tcgtgtcgtt
    26881 actgacgtct gcggcctgtg ctgtttgaat gccaaccatc ggggtggcca aaatcgctgc
    26941 agcggccaca atcttggcta tctttcgcat cggtgccctt cccagtcgaa tcggctgttg
    27001 acctgcgtca ggttacgcgc agcgcgtcgt ggcgaaggcc gaatcatcga accggctgtg
    27061 gcgcaatctg gttcaccggt cgagcagtgc ccgccgcagc aggtgcattc cgacggtcgt
    27121 cgaccgttcg cggacgtcgg agcggtttcc gggcagccgg agcgttcggg tctgcgtcgg
    27181 cccgcctgcg agcatcacgc tgaagcagac tgttcccacc ggcttgtcct ccgttccccc
    27241 gccggggccg gcgactccgg taatcgcgat tgcggtgtct gcgtcgaagc ggcgcagcgc
    27301 gccttcggcc atcgactccg cgacgggctc agacaccgct ccgtgcctct cgatcagcgt
    27361 ggcgtccaca ccgagcactt ccgtcttcgc ctcgttggca taggcaacga cgccgcccac
    27421 cacgtaatcc gacgacccag gccgctccgt cagtcgtgcg gccataaggc ctgcggtgca
    27481 cgactccgcc gtcgctatcc gacgtccggt caacagcgtc gccacctgat catcgacgcg
    27541 ggagccgtcc tcggagaaga tttcccgccc atggcgctgc cgcaacacct ccatcaggtt
    27601 cgcgtaaacc cctgtggcgt cgggctcgta acgcgtgacc atctcgatct ccccgcgccg
    27661 cagacacgtg gtgatctcca gccgctcgaa gcccggcacg tcttgttcag cctcgcgcag
    27721 agtttcagcc agcccggatt ccggcagtcc gaacatccgc accgtctcct gccggtacgt
    27781 ggtccgcccg gcgagcgcct cctgcacggc gtcggtctgc atggccttcg gccacatcgg
    27841 ctggagttcg cgcggcgggc ccggcaacac gacgaccgtc ggcttgaaag gcaccaccac
    27901 cccgggtgcg gttcctaccg ggtcgatcac gtgggacccg gttgggatca tcgcttgttt
    27961 gcggttcgcc gcacgcacag cgtccaggtc cacgttggcg aaccgcgcca tcagtcgctt
    28021 gaggatgtcg gtgatcttgt cctcgagttg cgcgtcgagc accagttcgc gtccacagaa
    28081 gcgtgcgacc gtcgcgaccg tcatgtcgtc ggccgtcggg cccagcccac cgctggtgac
    28141 gatcagatcg acgccctcgt ccgccagaaa gcgcagctgc gcttcgatgt cgccgggacg
    28201 gtcaccgcag atcgtgatgt gggcaagctc cacccccaat tcgagaagcc ggtctgcgat
    28261 ccagggtccg ttgcggtcct gcactcggcc ggtcagaacc tcggtccccg tcacgacaat
    28321 gcctgcgcgt gcgctcaccg gacaaaccta cctgcgagag gatgtgtcga tgatcgacat
    28381 agaggttcaa caagctgacg tcaccaagct cgaggtcgac gcgatagcca acgcggccaa
    28441 cacgcaactg cggcacggcg gcggcgtcgc ggcggcgatc gcacgcgccg gcgggccgga
    28501 tgtgcaacgc gaatcgaatg agaaagcacc gatcggtctc ggcgaggccg tcgaaaccac
    28561 ggccggcgag atgcctgcgc gttgggtgat tcatgcggca acgatggaac taggcggacc
    28621 gacatcgtcc gagatcattt cgcgcgcaac gcgttcgaca gtacgcaagg ccgacgaact
    28681 cggctgccgg tccctggcat tggtggcgtt cggcaccggt gtgggcggct ttccgctcga
    28741 cgaagcggcg cgattgatgg tgggcgcggt ccgcaagcac gagccgcagt cgctctcgag
    28801 tgtggtcttc gccgtgcacg gcgctgcggc cgagcaagcg ttccgtgccg cgctggattc
    28861 ttagctggcg ccgagatcga cgttttgcag cgccccactc ggactttgtc tgtaatacgt
    28921 cgatttcggc gtcatttacc ggtcacgttg ggcctcaagc tgtttgagaa ggccatcgag
    28981 gagatagcac acctcgttga accggtcgta gccgaccagg ccggcccaat gctcttcgat
    29041 ctcggtcact cgcacggtgg cggcgtcgag cgcttggtgg ccgcgccagg tcgccaggtc
    29101 ggtgccgcag cgttgtcact attgccaccc cggtgcacgc gctcagtcga cgcgagcgaa
    29161 tggtgatcgt cccgatggtg tgggcctacc gtttcgtcgg tgcggcgccg cccctggcga
    29221 acggagtagc gcgcgctctt ctcggccgag cgttcatgcg aacacggccg gaggacgccg
    29281 cagccgtcgt cgcgtctttc cggaacgctc cccgctccgg catgtaccgc gcgatgacgt
    29341 cggtgatgct caaccgcgcc gacctcgacc cgatcttgcc gcagatcacc acaccacgct
    29401 gatggtcgtt cccaccgccg acgcgatgct gcctgcccat cagattcatg cggcggtacg
    29461 ccaaatgcct tgcgccgcgt ccatcgaagt caaagccgaa gggcatgtgg cgccgatcat
    29521 cgcgaatgcg gagcagttgg ctgacatgat cacggctttc gggcgcgacc cgaaaggcta
    29581 tgtctcacgt caagtgcggg attagcctga agcggtggcg caggctccgc ggacccgcta
    29641 cgcgaggtgc ggcgagatgg acatcgccta tcaggtcttc ggcgacggtc cactggatct
    29701 gctcgtactc ccgggaccct tcatcccgat cgattcgatc gaggatgagc cgtcgatgta
    29761 tcgctttcat cggcgtcttg cgtcgttcag ccgggtgatc cggttcgacg tgcgcggaat
    29821 cggcctgtcg tcacgggtgc cgtctctgga cgtgatcggg ccggagtact gggctcagga
    29881 cgcgatcgcc gtcctcgacg ccgtcgggtc tgagcaggcg acgatcttcg caccgagtta
    29941 caccgcgacg agcgggatcg tcctcgccgc tgactacccc gcgcgggtgc acagcctggt
    30001 catcatgaac ggcgccgcgc gcgtgatgtg ggcgcccgac taccccgccg ggactcggct
    30061 ggaagccagc gaactgttca cgacggtcgc aacggagccg gacgcagtcg agcagggtgt
    30121 cgacgtgctc aaggtcgttg cgcccagcgt cgccgacgat caagcgttcc gcgcatggtg
    30181 ggaccgggcg ggcaaccggg cggcatcgcc gagtatggcc cgcgccgtct ccgacgtcgt
    30241 gcaaacagcg gatgtccgcg accggcttgc gcgcatcctc gcgccgaccc tgatcatgca
    30301 ccgcgccggc tcgggattcg ttccggtcgg gcacggtcgc tacctcgccg agcacatcac
    30361 cgcagcacgc tatgtcgagc tgcccggcgc agacgtcccg tactgggtcg gcgacaccgc
    30421 cgagatgctg gacgagatcg aggaattcat caccggcatg cgtggccgct tcgacgccga
    30481 gcgcgtgctc accacgatcg tgttcaccga catcgtcggc tcgacccggc gcgcagccga
    30541 actcggcgac gttcgttggc atgacctgct cgacaagcac gacagcatcg tgcgccacga
    30601 actcgagcga ttccggggtc gcgaaatcaa cacggccggt gacggattcg tcgcgacatt
    30661 catcagcccc agcgtcgcaa tcgactgcgc ggaagcgatc gtcgacgcgg tccgggcgct
    30721 cggcgtcgag gtacgggtgg gcatccatgc cggcgaggtc gaggtgcgcg gcgccgacgt
    30781 cgcgggcatg gcggtgcata tcggtgcacg cgtggcggcg cacgccggac cgagcgaggt
    30841 gtacgtgtcg tcgacggtgc gcgagatcgt caccggatcg cggcgcacgt tcgtcgaccg
    30901 gggcgagtac gaactcaagg gcgttccggg ggcctggcgg ctgtaccggg tcgcgcgcga
    30961 ccacatggca gggtctgtgc catgagcgac acgcagcagg ccaccgagga acccgttgaa
    31021 cggacgttgt tcggtcaccc catcgggttg gccaacctgt tcggagtcga actgtgggaa
    31081 cggttttcgt tctacgggat gctcacgatc ctcggctact acctgtatta ctcggtgacc
    31141 gacggcggac tggggctgcc gaagagcacc gcaacgggca tcgtcggcgc ctacggcggt
    31201 ctggtgtacc tgtccacggt tctcggcggc tggatcgccg accggctgct cggcatggag
    31261 cgcacggtgc tctacggcgg cgtcgtcgtg atggccgggc acatcgcgct tgcggtggtg
    31321 ccggggctgc ccggcgtcgg gatgggcctg gtgctggtcg cactcggctc gggcgcgctg
    31381 aaggccaacg catcgtcgct tttgggcacg ctgtacgaga agggtgatgc ccgcgccgac
    31441 ggcgggttca cgctgttcta tctcggcatc aacctgggcg cgttcatcgg tccgctgatc
    31501 accgggctgc tgcagacaca cgtcggcttc cattacggtt tcggcgctgc ggcgatcggg
    31561 atggcggctg ggctggcgca gtacgtcgtg ttccgtcgga atctggggac acatggacgt
    31621 gatgtgccga acccattgcc ccgcagcgct attagtcggg tcgccgctct tgttgtggca
    31681 gtactcgtgc tgatcgccgt ggtgctactg accggcgtcg tccggttgtc gaacctgtcc
    31741 caggtcacga ccggggtcat cgtcgtcgcc tccgcggcgt acttcgtgat catgctgacc
    31801 aactcgcggg tgacgccgac cgaacgatcg cgggtgcgcg ccttcattcc gctgttcgtc
    31861 gcgaacgcgg tgttctggtc gctgtttcag cagatcttca ccgtgctcgc ggtgtactcc
    31921 gacgaacgga tgaactggtc catcttcgac tggactgccc cgtcgaactg gatcggctcg
    31981 atcgaaccgg tctggatcat cgtgctgtca cccgttttcg cgatcatgtg gacgcggctg
    32041 ggcacgcggg cgcccaccac cccgcgcaag ttcgcctacg gcgtgatcgg gatggggctc
    32101 gcgttcctgc tgttcctccc gatggccggc accaccggca aagccgtccc ggctcttctg
    32161 gtggtcggca tcatggcggt gttcgcgatc tccgaactgc tgatctcgcc gatcggcctg
    32221 gcggtcacca caaagcttgc gccggaagcg tttcgggcgc agatgatggc gttgtacttc
    32281 ttctccgtcg gcctcggcac gtcgatgtcc ggtgtgttgg cgcgttacta cgatcccgac
    32341 cacgaattcg cctacttcgg catcatcgga gccgtcgcga tcgtggccgg cgtggtggtc
    32401 ttcgcgttga cgccatgggt gagccgccgg atggaaggcg tgcattagca gccatatccc
    32461 acggcgactg tgcggtttcg tacgcgacgc gccggggcgg gcgtacgaat tcgcacactc
    32521 ggatttcgcc ggcgtgtcgc gtgcagatgc gcacgctcgc gggaattaga actgcctgtg
    32581 tgggacgtcg gccaccaggc caccgtccat cacgaactcc gcgccggtgg cgaacgagga
    32641 ctcgtcgctg gccaggaaca gcacgaacgt cgccacctcc tcgggcgtgc cggggcggcc
    32701 catcggcgcc atcaccatgt cgtcgggcag gtgcttggtc atcggcgtgc ggatgaaccc
    32761 gggatggatc gagttgaccc ggatcttctg cgccgccagc tccaacgccg cggacttggt
    32821 gaggccgcga accgcccact tggacgccac gtagccgtgc acccatggcg caccgcgcag
    32881 gccctcgatc gacgaaatgt tgatgatcga gccgccgccg gcttcggtca tcgggtcgat
    32941 gacggcctgc atccccagga aggtgccggt caggttcacg tcgaggacct gctgccactt
    33001 cttcaggtcg tgggtcctca gcggccccag cgcgacggtg ccggcgttgt tcacgagcac
    33061 gttgagcttg ccgaactcgt cgacggccgt ggatactgcc gccttccact gctcggggtc
    33121 ggtgacgtcg aggtgcacat aacgcgctgc gtcaccgagg tcgtcggcca gcgctttgcc
    33181 ctcgtcgtcg agaatgtcgc cgatcaccac cttggcgccc tcggccacca gcatccgcgc
    33241 atgcgcagcg cccatgccgc gcgcgccgcc gctgatcagt gcaacttttt cgtccacgcg
    33301 tcccatgatg ggtcacgcta cctcagcaac tagaacctgt tgcaattcgg tgagagaaca
    33361 gcgccttgcc ggtgatcagc ggcaggtcga gcgtcgttct gatgcccggt ggcgccgcga
    33421 cgacggcggg gatcgcgttg acgatgcggc ccgcggcggc gacgatcgcg gcgtggttgt
    33481 ggtcgcccct gctgctcgac gggcagatgt ccaccttgta tgacggctca ccgacgattt
    33541 ccacccggta cgagccgccg ggctgcgccg gctggggcca gtcggggcgc aggtcttcgc
    33601 gcaggcgggt gatgtgctcg acgacgattg cgggccggcc gccgagcatg ccgcggatgt
    33661 cgaaccgcag cgctgccacg gtgcccttgg cgatgtgtcc cgcggcgatg tcgaacgctt
    33721 cgggcgccgg ctcccgcgtg cacgaatcgg tgatctcgtc gagctcgatg cccagcccgg
    33781 cggccaactg ccggatcgcg caaccccagg ccatgctgag cacgccaggt tggaatagca
    33841 gcggaacctc gtcgagcggt ttgccgaagc ccatcacgtc gaacatgacc gtggcgccgt
    33901 cgtaggtggc gtagtccgcg atttccatgc agcgcacctg ctcgatgcgc tggcatgtct
    33961 gcgcgaacgc cagcggtatc aggtcggtga cgaaccccgg atcgacaccg ttgatgaata
    34021 tcgtcgaatc actttgccgc gcagcttctt ccagcggctc gatgtactgc tgcggtagca
    34081 catgccacgg gtactgcagc agaaccggcg cggagccgac gacattgaca ccggcggcca
    34141 gaatgcgtcg gacatcgtcc atcgccgcag gcagcctgtt gtctcccatc gcgcagtaga
    34201 ccgcgcagtc gggcttgtcg gcgagcaaag cgtcgaggtc gttggtggcg gcgacgccgg
    34261 tgtgcacgtc gaggccggcc agctcgccgg cgtccttgcc caccttggcg tctgacgaca
    34321 cccacagccc ggtcagctcg aactgaggat tggtgaggat tccggtgagc gccagccgac
    34381 cgacgtttcc ggtgccgatg tgtgcgacgc ggattgccat gctcggcacc gtagcaaggt
    34441 gatacccgct accgtgctag cagtgcgcgt tccagtgtca gactcggttg cggcgatcgc
    34501 cgcaggtccg gcagggccgc gcgtcggcgc gttcttcgac ctcgacggca cccttgtctc
    34561 cggcttcacc gcgaccgttc atgccagcga tcggatccgg cgcggacaag cccgagtcgg
    34621 cgaagtgctc ggaatcctcg aagcatcact gaggtaccga ttcggccgga tgcagttcga
    34681 gcggttgctc gtcagggccg cgggctatct gcagggcgag tcgatacatg agctcgacga
    34741 actcggcgag cagctgtttt cccgccatgt cgcagcacgg gtctacccgc acatgcgcga
    34801 aatcgttcag gcgcaccaac agcgcggcca taccgtcgtg ctgagctcgt cggcgctgac
    34861 catccacgcc gaacccgtcg cccgtttcct tgggatatct cgggtggtgt gcaaccaatt
    34921 cgaactcgac gagcacgggg cgttgacggg acagatcgtc cggcccgtcg tctggggatc
    34981 gcaaaaggcc accgccgtcc aacggttgtg tgtcgccaat gacattgacc tgcaacgcag
    35041 ttacttctac gccgacggcg acgaagacat cgcgctgatg tcattggtcg gccatccccg
    35101 accggtgaat ccgcgacccg ggctcgccgc gatggccgca cagcgcgggt ggccggtgct
    35161 acgagtcacc agtcccgacg ggcgcacgcg ggcacggtcg ctgcgcacgg tggccagcgt
    35221 cggctggtcg gcgctgatgc gttacgccac cagcaccacg ctgcgccgcg agtgatgagc
    35281 gtcgccacgc tcttgcccgc cgcgtgcgcc ggcttcttgc ttgccgtgct gtggatggac
    35341 ctcatcttcg acgtgcaggt ggtggcgcac cgccgctccc tcggggacct acccgaatcc
    35401 gtattggcgt ccatcgccgg gtactaccgc cgcgcgacca cgcggccgat gagcatgctg
    35461 atcgccgtgg tcatgttgat gctgttggcc gcgttggcgg ttcggggggc atccggggac
    35521 cccgggtggc tgattgccac gtgggctgcg ttagccggcg gtccgatcct gttggcgctg
    35581 attcgcacgg tgcccaatgc cgttcgactg ggacggcggg cggacaccgt cgccgatcag
    35641 agccggctcg cgcgggcgat ctttcgcgat cacgtggtgt gctttgcgct gatgctggcg
    35701 ttcctcggct tgtggctcgc cgcagctagt ctcaggttgt gacgacaaca cttcgcgacc
    35761 ccaaagtcgc gcatgccctc gatcggatgt atcgcgaggc caccgagcag atgaccgcgc
    35821 ggcagtggcc agaccttcag tcgtcggcaa gcgctcagga gcgcgctgac gcgctcagcg
    35881 acttctacct gcccgtcacc cccgaagccg ggcggttgct gtattcactg gtgcgggctg
    35941 cgcgcccagg cactatcgtc gaattcggca tgtcacttgg tatttcggcg attcaccttg
    36001 cggctgcggt gcgggacaac ggccacggcc aggtcgtcac caccgaactc agcgccgcca
    36061 aggtcgccgc tgcgaagaag acgttcgccg acatcggctt ggacgacctg atcaccgtgc
    36121 tcgagggcga cgcgctgagc acgctggaag cagtcgacgg ttcggtggac ttcgtgcttc
    36181 tcgacggctg gaaagagttg tatctgccgg tggtcaagct gctcgagcca cggctgtcgc
    36241 ccggggcgct aatcgtcgcc gacaacaccg aaatggcaga caccagatcc tatttggacc
    36301 acgttcgcga tcccgacaac ggctacgtga gcgtcaactt ccgcgcccgc gacgccgaca
    36361 gcatggaaat cacctgccgc gcaacgactt gactacgtaa actgcggtgg ccgtcgctgt
    36421 gcccacggag cggtgagctc cagttggcgc gcgagccgca acagcagctt ctcaccgctg
    36481 agtggcgcga cgaactgcac acccatcggc agtccggacg cggtccagtg cagcggcagg
    36541 ctgatggccg ggcggccggt gatgttggcc agctgcgtgt acggaaccca gctcaggttg
    36601 cgtctgatga tctggtcggc gatgcctgtc gattcgagga tccggacggt tcgcgtgcgt
    36661 aggaacccgt ttgcgatagt gcggagaacg gcgggaagct ccagtgatcc gatccggatc
    36721 ggcggttcgc cgagtgtggg tgtcatcaga aggtcgtaac tggcgtgaag ctgcgccaat
    36781 ccggcgatgt gatcatggcg tcgttcctgg gcttcctgca gtgcgacgcc gctgaccgct
    36841 cgtcccagcg cggccatcat gcgtgtgtcc tgttcgaagc cgtcgtcttt ggcgccggtc
    36901 tcgcgtttga tgcgcgcgat ctcgatcgcc tgggccacga accagacggt gaggaagtcg
    36961 tgcgcgagtt ggtggtcgtc ccacgacggg gccacctgct cgacgtcgtg gccgagctct
    37021 gccagcagct gcgccgctgc ctcgaccgca cgggtggcct ccgggtgagc cgagccgcgg
    37081 atggccgagt gggtggtgaa cccgacgcgc aggcggtccg gtggccggtt gaggtcgtca
    37141 aggaatggcc gatcgggtgt ggccggcgcg aaggcggaca ctgcgtcggg gccggccagt
    37201 acgtcgagca tggccgcggt gtcgcgcacc gaacgcgaga tcaccccgtt ggtcgcttgc
    37261 ccgttgaggg gatcgctggc atccggtccg gacggaatca agccacggcc cgccttcagg
    37321 ccgaccagcc ccgtgcacgc ggcggggatg cggatcgacc cgccgccgtc gctggcgccg
    37381 gccaccggga tgatgcccgc cgcgacggct gccgccgcac cgcccgacga gccgcccggc
    37441 gtgcggtcga gatcccacgg attgcgtgtc ggcccaaaga gttcgggctc ggtgatgccc
    37501 ttggcgccga attccggggt gttggtcttg ccgaatatca ccacgccggc gtcgagccag
    37561 cgctgcacga cggtgctgtt gtcgaacacg gttcggtcgg ccagagcgcg gcagccatcc
    37621 gtcgtcgtcg tccccgccaa gtcttggtgg aggtctttca gcaggaaggg cacaccggcg
    37681 aacggcccgt gcagttcgcc gctcacccgc tgttcggcac gctggtccag gcgagtgcag
    37741 atcgcgttga tcttcgggcc gaaccggtct gcgcgggcct gggcgatgcg aagaagctcg
    37801 gcggcttgga cttcgccgct cgccaccagc tcggccaggc cgagcgcgtc tagctgcgtg
    37861 tactctcgct cgtccacagt ggcgaaccta gcgcagttcc tggcatagcc ctacggaagc
    37921 cttgaatgca ctgcgcgccc ggcgttttca tctcggcggg cgcgcagtgt gtccatcaag
    37981 gaagcgcgga gaacggattg aggttcggcg gcaacgcggg ctggccgggc gccgccggca
    38041 ccgcgggagc cgtgatgggc gcgccggggt ttgcacccag gttcggtatc ggaaggctgc
    38101 tcaggttgcc cggcagcccc aactggttga gcagcggcgc catcggaaca gatgcgggca
    38161 ggttgagttg gccctgcggc acggtcccga cgagggagtt catcggtgcg gatatcggcg
    38221 tcggcgtggt agcagccggc gctgagactg ccggcgctga gactgccggc ttcggcgcga
    38281 gcatcgacgc ggcattttgc aagaactgag acgccatcgc cggggcgttg cccagttgct
    38341 gcatcaggtc gatacccggc atgccgggga cggcgggtgc cgagtccgcg ccggcggtcg
    38401 ggctcagcgc gacaaaggcc gtgaccaggc ctcccgctgc ggctgcgatg gtggcaatgc
    38461 gagtgccgat gcgtgacatg tgttccccac tccagttgac gtcaagaccc aacccgaagc
    38521 taggggtgtt acggaagtta ctcaagtgac gcgtgtggcc attaccgaac cgatatgaac
    38581 cagacgcgcg gaggttatgt gggctaccgc tgcccgcaaa accagcgttt cgcacagaaa
    38641 gtgcgcaaac gtgcgacacg tcggtttcgc gggaagggaa gggcgggcag agacccgccg
    38701 gtgcccccgc cgctgcttgg tactaaacta gaacacgttt cagttcgcgg cccgcgtcca
    38761 aaggagcagg caatgcacac cgccatctgc gacgaactcg gcatcgagtt tccgatcttc
    38821 gcattcacgc actgccgcga cgtggtcgtc gcggtgagca aggccggcgg cttcggggtg
    38881 ctcggcgcag tcggcttcac gccggaacag ctcgagatcg aactcaactg gatcgacgag
    38941 cacatcggcg accactccta cggcgtcgac atcgtgattc cgaacaagta cgagggcatg
    39001 gactcgaacc tgtccgccga ggaactcacc aagacgctgc agtcgatggt gccgcaggag
    39061 cacctggact tcgcccgcaa gatcctggcg gaccacggcg ttcccgtcga ggatagcgac
    39121 caggacg
//
LOCUS       DASWBF010000175         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_78360, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000175 DASWBF010000000
VERSION     DASWBF010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78360"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..749)
                     /locus_tag="VF874_06125"
                     /pseudo
     CDS             complement(1..749)
                     /locus_tag="VF874_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559394.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
     gene            complement(804..1619)
                     /gene="tsf"
                     /locus_tag="VF874_06130"
     CDS             complement(804..1619)
                     /gene="tsf"
                     /locus_tag="VF874_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX9175678.1"
                     /translation="MANFTAADVKRLRELTGAGMLACKNALSDSDGDFDKAVELLRIK
                     GAKDVGKRAERSTAEGLVAAKDGALIELNCETDFVAKNDEFQKLAEDIVSAAVSSGAA
                     DVDALTGASVGDKTVEQAIADMSAKIGEKLELRRVAAFDGTVETYLHKRSADLPPAVG
                     VLVEYTGSDASAAHAVALQIAALKARYLSRDDVPEDVVADERRIAEETARNEGKPEQA
                     LPKIVDGRLNGFFKDVVLLDQSSVSDNKKTVKALLDEAGITVTRFARFEVGQA"
     gene            complement(1621..>2329)
                     /gene="rpsB"
                     /locus_tag="VF874_06135"
     CDS             complement(1621..>2329)
                     /gene="rpsB"
                     /locus_tag="VF874_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9175679.1"
                     /translation="IDKAYEFVKETVAHGGTVLFVGTKKQAQESVAAEAIRVGMPYVN
                     QRWLGGMLTNFSTVHKRLQRLKELEAMEQTGGFEGRTKKEILGLTREKNKLERSLGGI
                     RDMNRVPSAIWVVDTNKEHIAVGEARKLGIPVIAILDTNCDPDLVDYPIPGNDDAIRS
                     AALLTKVVAAGVAEGLQARAGLGNGDKGEAEGAEPLAEWEQELLASATAAAAANAPED
                     APTNPGPTATETKTTEG"
BASE COUNT          335 a          830 c          729 g          435 t
ORIGIN      
        1 agccgttcgg tcccgggatg gctgacatcg ccgatcggcg gtaacttccg acagcgccaa
       61 acctgttcgg cctcacggta aaacgccgct gtgtcgcgca ccgagcgggt caccacgccg
      121 ttgtggacga cccgcagcgg catctgccgc gtgtgcttgt ccagcagcag ccgcccgcgg
      181 gtgggtttca accccactaa tccgttgcaa gacgcgggaa ttcggatcga gccgcctccg
      241 tcgttggcgt gcgcgacagg caccacgccg gctgcgacga acgcggccga gcccgacgac
      301 gacgctcccg cagtgtggtt cgggttccac gggttccgca ccggaccgat gcgcgggtgc
      361 tcggccgcgg cgctgaaccc gaactccgac agctgcgtct ttccgagcgg gatcaacccc
      421 gtcgccagga acgcacgcgc gaaatcacca tgagcccgtg caggcgtcgg gtcccacgcg
      481 tccgtgccct gcatggtcgg catgccggcc acgtcgacgt tgtccttcac gaatgtcggg
      541 acgccggcga agaagccgga ctgctgcgta gacgcctggc gccgggcctg ttcgaacgcc
      601 tcatgggcga ttccgttcaa agccgggttc accgcttctg cgcgtgtgat cgccgcgtcc
      661 actaactcag aagccgacac ctcaccggag cggatccgct cggcgagccc gacggcgtcg
      721 tcgtccccta acgcgtcgtc gcgaaaggca tgacatcatg ttgttggctc ttcgcgcaag
      781 cggctcatcg cgcgacacca tgattacgct tgcccgacct cgaagcgcgc gaagcgggtc
      841 accgtgatgc cggcctcgtc gagcagtgcc ttgaccgtct tcttgttgtc ggataccgat
      901 gactggtcaa gcagcacaac gtctttgaag aagccgttca gacgcccgtc gacgatcttc
      961 ggcagcgcct gctccggctt gccttcgttg cgcgcggtct cctcagcgat gcggcgctcg
     1021 tcggccacca cgtcctcggg aacgtcgtcg cgcgacagat accgagcctt caatgccgcg
     1081 atctgtagtg cgaccgcgtg cgcagccgag gcatccgagc cggtgtactc caccagcacc
     1141 ccgactgccg gcggcaggtc ggccgaccgc ttgtgcagat aggtctcgac ggtgccgtcg
     1201 aacgccgcga ccctgcgcag ctccagcttc tcgccgatct tcgccgacat gtcagcgatc
     1261 gcctgctcga cggtcttgtc cccgaccgac gcgcccgtca gcgcatccac gtcggcggca
     1321 cctgacgaca cggccgcgga aacaatgtcc tcggccagct tctggaactc gtcgttcttc
     1381 gcgacgaaat cggtttcgca gttcagctcg atcaacgcgc cgtccttggc cgcgaccagt
     1441 ccttctgcgg tcgaccgctc cgcgcgcttg ccgacgtctt tcgcgccctt gatgcgcagc
     1501 agttcgacgg ccttgtcgaa atcgccgtcg ctgtcgctca gcgcgttctt gcaagccagc
     1561 atgccggcgc cggtcagctc ccgcagtcgc ttcacgtcgg cagcggtgaa gttagccatc
     1621 tcagccttcc gttgtcttcg tttcggtggc ggtcggaccg ggattcgtgg gtgcgtcttc
     1681 cggggcgttc gcggccgcgg cagcggtggc gctggccagc agctcctgct cccactcggc
     1741 cagcggctcg gcgccctcgg cctcgccctt gtcgccattg cccagcccgg cgcgcgcctg
     1801 cagaccctcg gcgacgcccg cggccaccac cttcgtcaac agcgcggctg agcggatcgc
     1861 gtcgtcgttg cccggaatcg ggtagtcgac gaggtccggg tcgcagttcg tgtcgaggat
     1921 cgcgatcacc gggatgccca gcttgcgggc ctcgccaacc gcgatgtgct ccttgttggt
     1981 gtcgacgacc cagatcgccg acggcacccg gttcatgtcg cggataccgc cgaggctgcg
     2041 ttccagcttg ttcttctcgc gggtcagccc gaggatttcc ttcttggtgc ggccctcgaa
     2101 cccgccggtc tgctccatcg cctcgagctc cttgaggcgc tgcagccgct tgtgcaccgt
     2161 ggagaagttg gtgagcatgc cgcccagcca gcgctggttc acgtacggca tgccgacgcg
     2221 gatcgcttcg gccgcgacgg actcctgcgc ctgcttcttg gtgccgacga acagcaccgt
     2281 gccaccgtgg gcgacggtct ctttgacgaa ctcgtacgcc ttgtcgatg
//
LOCUS       DASWBF010000176         8446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1265573,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000176 DASWBF010000000
VERSION     DASWBF010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265573"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF874_06140"
     CDS             complement(<1..481)
                     /locus_tag="VF874_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX9175680.1"
                     /translation="MSVDVDVAKIVHPPCGALRGTTEFGVGVWRGVPYAEQPVGERRF
                     TAPAPLPPWRGVRDALEHGPIPLQGKSYVGRGRDDPKVRDEACLTLTVWSPDTAASLP
                     VMVWIPGGAFVLGAGQFQLYNGARLAANGDVVVVNVTYRIGVFGGLELSSLGDGFDDN
                     "
     gene            543..1427
                     /locus_tag="VF874_06145"
     CDS             543..1427
                     /locus_tag="VF874_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX9175681.1"
                     /translation="MTFNEGMQIDTSTASSSGGGPGRGLAIGGGVGGLVLVVVALLLG
                     VDPGTIPMEPQQGQQQEVSGFDISQCKTGADANKILGCRIIATGNSVDAVWSEQLQGY
                     TRPEVHLFSGSVQTACGGATTEVGPFYCPADKTAYFDTDFFQVLVDQFGSSGGPFAQE
                     YVVAHEYGHHVQNLLGDLGRAQQSGAQGAEGAGVRTELQADCYAGVWAHYASTVKEQS
                     TGVPYLKPLTDKDIQDALSAASSVGDDWIQKQSTGRVNPESWTHGSSEQRQHWFTVGY
                     QLGDPKRCDTFNASDLDG"
     gene            complement(1431..2474)
                     /locus_tag="VF874_06150"
     CDS             complement(1431..2474)
                     /locus_tag="VF874_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HEX9175682.1"
                     /translation="MRWDGQSVRVDDGALPGLQRIGFVRSVRTPQFEGITFHEVLCKS
                     AINKIPDAAMLPFKYTVNGYRGCSHACRYCFARPTHEYLEFDPGNDFDREIVVKTNVA
                     DVLHRELHRRSWQRDTIALGTNTDPYQRAEGRYALMPGIIGALADSGTPFSILTKGTL
                     LRRDLPLLTAAAEQVPISLAISLAVADPEFDVAGRAAPAVHRDVEPGTPSSQARLGLI
                     AAIREAGFDCHVMVAPVLPHLTDSAEHLDALLGQIAKAGATSVTVFGLHLRGSTRGWF
                     MSWLGRSHPELVSRYRELYRRGAYLPQDYREMLRRRAAPLVSKHGLTGDRREFTAPPA
                     AAEKVAAVQPTLF"
     gene            2539..2877
                     /locus_tag="VF874_06155"
     CDS             2539..2877
                     /locus_tag="VF874_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX9175683.1"
                     /translation="MTELLFSYGTLRQRGVQLATFGRELDGRDDAIVGFELDYLTITD
                     PHVIATSGSDRHPILRPTHQPGAEVQGTVFAISEAELAAADEYEVDDYARVLVPLRSG
                     QHAWVYVFAG"
     gene            complement(2888..3301)
                     /locus_tag="VF874_06160"
     CDS             complement(2888..3301)
                     /locus_tag="VF874_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175684.1"
                     /translation="MLKVATRFGAALLAAAGLMSTQAIASAEPAGHQVRYTITTGQEA
                     TVNLYYLATEPETQAAYDANPNAYLRNERVTVAPGAPWVFETTLKDTSWAYVMAGGAA
                     RYNGTPNPHCDIAIDGNVVAQQDGETAATCALKAW"
     gene            complement(3342..4595)
                     /gene="hisS"
                     /locus_tag="VF874_06165"
     CDS             complement(3342..4595)
                     /gene="hisS"
                     /locus_tag="VF874_06165"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX9175685.1"
                     /translation="MTEFQAPKGVPDYVPPASAEFVAVRDGLLAAARLAGYSDIELPI
                     FEDTALFARGVGESTDVVSKEMYTFTDRGDRSVTLRPEGTAGVIRAVIQHGLDRGPVP
                     VKLCYAGPFFRYERPQAGRYRQLQQVGIEAIGVDDPALDAEVIAVADAGFRALGLEDF
                     RLEITSLGDDTCRPQYRELLRDYLLKLNLDKETRQRARINPLRVLDDKRESVRSQLAD
                     APVMLDHLSDAAKQHFETVLALLDDLGVRYVVNPRLVRGLDYYTKTTFEFIHDGLGAQ
                     SGIGGGGRYDGLMRELGGQDLSGIGFGLGVDRVMLALRAEGKQVGATSRCDVFCIPVG
                     EESRREVAVIAARLRAAGVRVDVAYGDRGLKGAFRAADRSGAALAVIKDDADADVKLQ
                     DLGTRDQELVPAESIVGEVLSRLGR"
     gene            complement(4592..5257)
                     /locus_tag="VF874_06170"
     CDS             complement(4592..5257)
                     /locus_tag="VF874_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9175686.1"
                     /translation="MLITGFPAGMLACNCYVLAPRAGSDAIVVDPGQRAMAPLRRILD
                     EHRLTPAAVLLTHGHVDHIWSAQKVADTYGCPAYIHPADRFMLTDPIKDFGPRFGQLL
                     FGALFREPKQVIELDRDGDKIDCGGITVTVDHTPGHTRGSVVFRVDDGPDDVVFSGDT
                     LFQGSVGRTDLPGGSGRDLLGSIATKLLVLDDDTVVLPGHGESTTIGAERRTNPFLEG
                     LTG"
     gene            5347..6228
                     /locus_tag="VF874_06175"
     CDS             5347..6228
                     /locus_tag="VF874_06175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9175687.1"
                     /translation="MPTEKQRRATAKRKLERQLERRAQQDRRRRILTIAALAAVVVVV
                     AGVAVWAVFFRKGSDETPTASSTTSAPPTQDTATPQAGVLPPFRPPADLGANCQYQPT
                     PNEPAAKPVAPPNSGKVPTTPASISMSITTNQGPLGVQLNDAQAPCTVNNFVSLAQKG
                     YFNDTHCHRLTTAEDLYVLQCGDPKGDGTGGPGYQFADEYPSNQYLPDDPARNQAVIY
                     PRGTLAMANGGPGTNGSQFFIVYKDSKLPPNYTVFGKVDEKNMGVLDKVAAAGTVDGS
                     PDGKPKTDVVITSARLD"
     gene            complement(6303..6959)
                     /locus_tag="VF874_06180"
     CDS             complement(6303..6959)
                     /locus_tag="VF874_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175688.1"
                     /translation="MRHKRLTVFAAAGAVIAATALGASSATALAMPQSGTFVADMAEP
                     DGNTMALGIAVEGEMVTAYATDGTSDEAVFMGTQSNGTMTLNSPYQDQLTASFDGSNV
                     NGTITMNEPNAQPHNFSASLVSAPAGIYTVAAGDSRTTWVVKPNQTMVGMMMPNSKRD
                     REVIDQINAEQQEFKDKVRQMRLERQMQPAPQMTYGTWQTTMNGTTMTAVPVTGGMTL
                     "
     gene            complement(7086..7523)
                     /locus_tag="VF874_06185"
     CDS             complement(7086..7523)
                     /locus_tag="VF874_06185"
                     /inference="COORDINATES: protein motif:HMM:NF042550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6636 domain-containing protein"
                     /protein_id="HEX9175689.1"
                     /translation="MRVPLRLSIPVALVSTAMLSPGTAHAFSFYQFQSPSGNVFCGVG
                     TLDNGNAFAQCEIVDRSWSPPPRPMACEGEWGDRIGLNQGRPATFECHGDTLRGDGLP
                     TLDYGRSYFEGPVTCSSEESGVTCSDISTGHYFTISRESYGLG"
     gene            complement(7579..8446)
                     /locus_tag="VF874_06190"
                     /pseudo
     CDS             complement(7579..8446)
                     /locus_tag="VF874_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891994.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
BASE COUNT         1412 a         2828 c         2831 g         1375 t
ORIGIN      
        1 ggttgtcgtc gaagccgtcg ccgaggctgg acagttccag gccgccgaac accccgatgc
       61 ggtaggtgac gttgacgacg accacgtcgc cgttggcggc cagccgcgcg ccgttgtaga
      121 gctggaactg gccggcgccg agcacgaacg cgccgccggg gatccacacc atcaccggca
      181 gcgacgcggc cgtgtcgggt gaccacaccg tgagcgtcag gcaggcctcg tcccgcacct
      241 tcgggtcatc gcggccgcgg ccgacgtacg acttgccttg cagcggaatc ggcccgtgtt
      301 cgagggcgtc gcgcacgccg cgccatggcg gcagcggcgc gggcgcggtg aaccggcgct
      361 ctccgaccgg ctgctcggcg tagggcacac cgcgccagac gccgacaccg aactcggtgg
      421 tgccgcgtag tgcgccacat gggggatgca cgattttggc gacatcgacg tccacggaca
      481 cacacgaatc ctagtgtggg aagctggact cgacccggcc ggcgaagcga tggagcggtg
      541 cgatgacctt caacgagggc atgcagatcg acacgagcac tgcgtcgagc agcggcggcg
      601 gccctggccg cgggctggcg atcggcggcg gagtgggcgg gctggtgctc gtcgtggtcg
      661 ccttgctgct cggtgtggac ccgggcacca tcccgatgga accgcaacag ggccaacagc
      721 aagaggtgtc cggctttgac atcagccagt gcaagaccgg cgcggacgcc aacaagattc
      781 tggggtgccg gatcatcgcc accggcaact cggtggacgc ggtgtggagt gagcagctgc
      841 agggatacac ccgaccagag gtgcatctgt tctcagggtc ggtgcagacg gcgtgcggcg
      901 gcgcgaccac agaagtgggc ccgttctact gcccggccga caagaccgcg tacttcgaca
      961 ccgacttctt ccaggtgctc gtcgaccaat tcggttccag cggcgggcct ttcgcgcagg
     1021 agtacgtcgt cgcccacgaa tacggccacc acgtccagaa cctgcttggc gacctcggcc
     1081 gcgcgcagca gtcaggcgca cagggagcgg aaggcgctgg cgtacgcacg gagttgcagg
     1141 ccgactgcta cgcgggggtg tgggcgcact acgcgtcgac cgtcaaagag cagagcaccg
     1201 gtgtgccata tctaaagcca ttgacggaca aagacattca ggatgcactg tccgcggcat
     1261 cttcggtggg tgacgactgg attcagaagc agtccaccgg ccgcgtcaac ccggaatcgt
     1321 ggacccacgg ctcgtctgag cagcggcagc attggttcac cgtcggctac cagctaggcg
     1381 acccgaagcg ctgcgacaca ttcaacgcct cagatctcga cgggtagacg tcagaacagc
     1441 gtcggctgca cggctgcgac cttctcggca gcggcgggcg gcgctgtgaa ttctcttctg
     1501 tcaccggtta atccgtgctt ggagaccaac ggagcggccc gccgtctcag catctcgcgg
     1561 tagtcctgcg gaagatacgc gccgcggcgg tacagctcgc ggtagcggct gaccagttcc
     1621 ggatgcgagc ggccgagcca cgacatgaac cagccacggg tggagccgcg caggtgcagg
     1681 ccgaacacgg tgaccgacgt cgcgccggcc tttgcgatct ggccgagcag cgcgtcgagg
     1741 tgttcggctg agtcggtgag atgcggcagc accggcgcga ccatcacatg gcagtcgaag
     1801 cccgcctcgc ggattgcggc gatcagcccc agccgcgcct gcgatgacgg cgtgccgggc
     1861 tcgacgtcgc ggtgcacggc cggagccgca cgaccggcga catcgaactc cggatcggcg
     1921 actgccaacg agatcgcgag gctgatcgga acctgctcgg ccgccgccgt cagcagcggc
     1981 agatcgcgcc gcagcagcgt acccttcgtc aggatcgaaa acggcgtgcc cgaatccgca
     2041 agtgcgccaa tgattccggg catcaacgcg tagcggccct ccgcgcgctg gtacgggtcg
     2101 gtgttggtgc cgagcgcgat cgtgtcgcgc tgccacgacc gccggtgcag ttcacggtgc
     2161 agcacgtcgg ccacgttggt cttgacgacg atctcacggt cgaagtcatt gccagggtcg
     2221 aactcgaggt actcgtgggt gggccgcgcg aagcagtagc ggcaggcgtg ggagcagccg
     2281 cggtagccgt tgaccgtgta tttgaacggc agcatggccg cgtctgggat cttgttgatc
     2341 gccgacttgc agagcacctc gtggaacgtg atgccctcga actggggcgt acggacgctg
     2401 cggacgaagc cgatgcgctg caggcccggc agcgcgccgt cgtcgaccct gacgctttgg
     2461 ccgtcccacc gcatgccttc attcgaacgc ccgttcgatg cgctgtcaag tcgccacgct
     2521 cctgcttgac tgtccggcgt gacggagttg ctgttctcct acggcacgct gcgccagcgc
     2581 ggcgtgcaac tggcgacgtt cggccgggag ctcgacggcc gtgacgacgc gatcgtcggc
     2641 ttcgagctgg actacctgac gatcaccgac ccgcacgtaa tcgccaccag cggcagcgac
     2701 cgtcacccga tcctgcggcc aacgcaccag ccgggcgcgg aagttcaggg cacggtgttc
     2761 gcgatcagcg aggcggagct ggccgccgcc gacgagtacg aggtcgacga ctacgcgcgg
     2821 gttctggtgc cgctgcgctc aggtcagcac gcgtgggtgt atgtcttcgc cggctgagaa
     2881 ccagccgcta ccaggccttc aacgcgcatg tcgcggcggt ttcaccgtcc tgctgcgcga
     2941 ccacgttccc gtcgatcgca atgtcacagt gcgggttcgg ggtcccgtta tagcgcgcgg
     3001 cgccgccagc catgacataa gcccagctgg tgtccttcaa cgtggtttcg aagacccacg
     3061 gcgcgcccgg agcgacggtg actcgctcgt tgcgcagata cgcgttcggg ttcgcgtcgt
     3121 acgcggcctg ggtctccggc tccgtcgcca ggtaatagag gttgacggtc gcctcctggc
     3181 cggtcgtgat cgtgtaccgg acctggtgcc cagcgggctc ggcactggca atcgcttggg
     3241 ttgacatgag gcccgcggca gcaagcaacg ctgcgccgaa tcgggttgcg actttaagca
     3301 tgtaggtcac atcgccgcac gctaccggat cgttacatgt actagcgacc gaggcgagaa
     3361 agcacctcgc cgacaatcga ttccgcggga accagttctt ggtcgcgagt gcccaggtcc
     3421 tgaagcttca cgtcagcgtc ggcgtcgtcc ttgatgacgg ccaaggccgc acctgagcgg
     3481 tcggcagcac gaaacgctcc cttcagaccg cggtcgccgt atgcgacgtc cacgcgaacg
     3541 cctgctgcgc gcagtcgggc ggcgatgacg gcaacctcgc gccgggactc ctcgccaacc
     3601 ggaatgcaga agacgtcgca gcgagacgtc gcaccgacct gtttgccttc ggcacgcaac
     3661 gcgagcatca cgcggtcgac gccgaggccg aaaccgatgc ccgacaggtc ctgaccgccc
     3721 agttcgcgca tcagaccgtc gtaacggcca cccccaccga tgccggactg cgccccgagc
     3781 ccgtcgtgga tgaactcgaa cgtcgtcttg gtgtagtagt ccaacccgcg gacgagtcgg
     3841 gggttgacga cgtaacgcac gccgaggtcg tccaggagcg ccaacaccgt ctcgaagtgc
     3901 tgtttggctg catcggagag gtggtcgagc atcaccggcg cgtcggcgag ctggctgcga
     3961 acggactcgc gtttgtcgtc gaggacccga agcggattga tccgtgctcg ttgccgagtt
     4021 tccttgtcca ggttcaactt cagcaggtag tcccgcaaca gctcccggta ttgcgggcgg
     4081 caggtgtcgt cgcccagcga cgtgatctcc agccggaagt cctccaggcc cagcgcgcgg
     4141 aaaccggcgt cggccaccgc gatcacctcg gcgtccagcg cgggatcgtc gacgccgatc
     4201 gcttcgatgc cgacttgctg cagctggcgg tagcggccgg cctgggggcg ttcgtagcgg
     4261 aagaacgggc cggcgtagca caacttgacc ggcaccggtc cgcggtccag gccgtgttgg
     4321 atcaccgcgc ggatcacccc ggcggtgccc tcggggcgca gtgtgacgga ccggtcgccg
     4381 cggtcggtga acgtgtacat ctccttggac accacgtcgg tggactcgcc gacgccgcgg
     4441 gcgaacagcg cggtgtcctc gaagatcggc agctcgatgt cgctgtagcc ggcgagccgt
     4501 gcggcggcca gcagcccgtc gcgcacggca acgaactcgg ccgacgccgg tggcacatag
     4561 tcgggcacgc ccttgggcgc ttggaattcg gtcatccggt caggccttcg agaaacgggt
     4621 tggtgcggcg ttcggcaccg atcgtggtgg attcgccgtg tcccggtagt accacggtgt
     4681 cgtcgtcaag caccaacagt ttcgtcgcga tcgacccgag cagatcccgc ccgctgccgc
     4741 cgggcaggtc ggtgcggccg acggaccctt ggaacagcgt gtcgccggag aacaccacgt
     4801 cgtcggggcc gtcgtcgacc cggaacacca ccgacccgcg agtgtgccct ggggtgtggt
     4861 cgacggtcac ggtgatgccg ccgcagtcga tcttgtcgcc gtcgcggtcc agttcgatca
     4921 cctgcttggg ctcgcggaac aacgcgccga acagcagttg accgaagcgg ggcccgaagt
     4981 ccttgatcgg gtcggtcagc atgaacctgt ccgcgggatg gatgtacgcc gggcagccgt
     5041 aggtgtcggc caccttctgg gccgaccaga tgtgatcgac gtgcccgtgg gtcagcagca
     5101 cggccgcggg ggtgagccgg tgctcgtcga ggatccggcg cagcggcgcc atcgcgcgct
     5161 gcccggggtc gacgacgatc gcatccgatc ccgcccgcgg ggccagcacg tagcagttgc
     5221 aggccagcat tccggccggg aatccggtga tcaacacagg tccagtttcc cactgcctgg
     5281 ccgggcaccc agcgctgcat ggcacactcg gtggccatga gccctgaggt agaggaggac
     5341 agcggcgtgc cgaccgaaaa gcagcggcgt gcgacagcca agcgcaagtt ggagcgtcag
     5401 ctcgagcggc gcgcgcagca ggaccgccgt cgccggattc tgaccatcgc cgcgctggcc
     5461 gcggtcgtcg tcgtggtggc cggcgtggcg gtgtgggcgg tgttcttcag gaagggctcc
     5521 gacgagacgc cgaccgcgtc gagcacgacc tcggcgccgc ccacgcagga caccgccacg
     5581 ccgcaggccg gggtgctgcc accgttcagg ccgccggcag atctgggcgc gaactgccag
     5641 taccagccga caccgaacga gccggcggcc aagccggtcg ccccgcccaa ttccgggaag
     5701 gtcccgacga ccccggccag catcagcatg agcatcacga ccaatcaggg cccgctggga
     5761 gtccaactca acgacgcgca ggcaccatgc acggtcaaca acttcgtgag cctggcccag
     5821 aagggctact tcaacgacac gcattgccac cggctgacca ccgccgagga cctgtacgtg
     5881 ctgcagtgtg gcgacccgaa gggcgacggc acgggcgggc cgggctatca gttcgccgac
     5941 gagtacccct ccaaccagta cctgcccgac gatcccgcgc ggaaccaggc ggtcatctac
     6001 ccgcgcggca cgctggccat ggccaacggc ggaccgggca ccaacggcag ccagttcttc
     6061 atcgtgtaca aggattcgaa gttgccgccg aactacaccg tcttcggcaa ggtcgacgag
     6121 aaaaacatgg gcgtcctgga caaggtcgcc gcggccggca cggtcgacgg ttcgcccgac
     6181 ggcaagccca agaccgacgt cgtgatcacg tcggcgcggc tggactgagg caaggcgctc
     6241 gacttctcgg ccaaggggca atcacgagtg cgcgagctcg tcgcgtggca gcgcgattca
     6301 ggtcagagtg tcatgccgcc ggtgacagga acggccgtca tcgtggtgcc gttcatcgtc
     6361 gtctgccagg ttccgtaggt catctggggc gccggctgca tctgccgctc gagccgcatt
     6421 tgccgcactt tgtccttgaa ttcctgttgc tcggcattga tctggtcgat cacctcgcga
     6481 tcgcgcttgg agttgggcat catcatcccg accatggtct gattcggttt gacaacccaa
     6541 gtggtgcggg agtctcccgc cgcgacggtg tagatgccgg ccggcgccga aaccagcgac
     6601 gccgagaagt tgtgcggctg cgcgttcggc tcgttcatcg tgatcgtgcc gttgacgttg
     6661 ctgccgtcga acgatgccgt tagctgatcc tggtacggcg agttcagggt catcgtcccg
     6721 ttgctctggg tccccatgaa cacggcctcg tcgctggtgc cgtcggtcgc gtaggcggtc
     6781 accatctcgc cttcgacggc gatgcccaaa gccatcgtgt tgccgtccgg ttccgccatg
     6841 tcggcaacga aggtccccga ctgcggcatt gcaagagcgg tggccgagga cgccccgagc
     6901 gcggtcgctg cgatgacggc accggccgcg gcgaacacgg tgagcctctt atgtctcatg
     6961 gtttcttgct ccttctgtac gagataattg gcggatcggc gctcggggcg gctgaatcaa
     7021 cctgtcgagc gttggtgtgt gcgcgacggg ccggcgttac accaaagtcc cgccgcctcc
     7081 gcgcgtcagc ccaggccata cgactcgcgg ctgatcgtga agtagtggcc ggtgctgatg
     7141 tcgctgcacg tgacaccgga ctcttcgctg ctgcacgtga ccgggccttc gaagtaactc
     7201 ctgccgtagt ccaacgtcgg cagcccgtcg ccgcgcagcg tgtcgccgtg acattcgaag
     7261 gttgccgggc gtccctggtt cagcccaatc cggtcgcccc actccccctc acatgccatc
     7321 gggcggggcg gcggcgacca ggagcggtca acgatctcgc actgggcgaa cgcgttaccg
     7381 ttgtccagcg tgcccacgcc acagaagacg tttccggacg gggactgaaa ttggtagaag
     7441 gaaaaggcgt gcgccgtccc cggcgacaac attgccgtcg acaccaaggc caccgggata
     7501 ctcagtcgca gcggcactct catggttgct gttgtacacg cttttcacgt ccgcattggc
     7561 gtttcgacga actcgcggct agcgcggcgg ccgcaaggat ctcatcgcga cgcgcatgat
     7621 cggttcgggc acgcggtcct tcatcgccaa tgcccttgcg ccggcccggg ttcggaccgg
     7681 ggtgccgcgg ccgaacacct tgttcagcgg cccgcccgac ggttcgagct cgaaatgcag
     7741 cggcggttcc tcgccggcgg cacccagcgc gttggagctc acgacccgct ggatctcgcc
     7801 ggtgccctcg aagatggtgt acagcttggc gtctcggtac cacttctcaa cgggatggtc
     7861 ggtgatatag ccccgcccgc ccatggtctg gatcgcccgt tcggtggccc ggaccgcgac
     7921 ctcgctggcg gccagcttcg acatcgagcc ctcgccgcgc tcgaacagca cgccgttttc
     7981 cgccatccac gacgcccgcc acgtcaacag ccgcgccgcg tcgatctgcg tcgcgaactc
     8041 tgcgatcagg aacgtgatgc cctggttgtc gatgatcggc gcgccgaacg cctcccgctg
     8101 cttggcgtac gcggtcatgt attcgagtgc ggcccttgcg atcccgagtg cctgcgcagc
     8161 gaccaccggc cacgtctgct cgaacgtgcc cagcgtcgcc gagccgaaac gtcggccacc
     8221 ctcgatcgct tcacgcggcc tggccgatcg ccgaccgcac cagcgcaggc atcacgatcg
     8281 ccagcccaat gcccgcgcag ccccaaaaga cctcctccat gaacatcggc aacgacagac
     8341 cgatcgggtc gccgatcagg tcgcggtaga acagcgggct atagaaaccc cgttcggcgg
     8401 cctcctcgag cacgggccac gggaattcct ggcgcttgtc gaggtc
//
LOCUS       DASWBF010000177         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_539609,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000177 DASWBF010000000
VERSION     DASWBF010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539609"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VF874_06195"
     CDS             <1..564
                     /locus_tag="VF874_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX9175690.1"
                     /translation="TLRRAGERFGFAVRAMSLVAEHHDDESVAFSSTYIRSCVDAGDV
                     VAATEALGRPHRVEGVVVRGEGRGRVLGFPTANVAPPMYSAIPADGVYAAWFTVLGHG
                     PVVGTVAPGERYQAAVSVGTNPTFSGRTRTVEAYVLDTSADLYGQHVAIDFVARIRGQ
                     RKFAEVGDLIAEMEADTKRARTILLAR"
     gene            complement(561..1418)
                     /locus_tag="VF874_06200"
     CDS             complement(561..1418)
                     /locus_tag="VF874_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175691.1"
                     /translation="MGTYAVTGSASGMGRETAQRLRDGGHTVIGVDIRSAEVVADLST
                     PAGRKTAADRVLAASGAGLDGAMLAAGLGPAPGSDRPRQIAQVNYLGVVDLLASWRTA
                     LASTERAKVVVISSNSSTTVPAVPSRAVRALLTHDADKAVRAVRWFGKNASVMMYAAS
                     KIAVSRWVRRNAVTPEWAGAGIRLNALAPGAVMTPLLQEQLSTPKEAKAVRSFPIPIG
                     GFGDAGQLADWACFMLSDAADFLCGSVVFVDGGSDAYFRANDWPKAVPAYRLISFLRR
                     FRGGQALAG"
     gene            1593..1862
                     /gene="rpsO"
                     /locus_tag="VF874_06205"
     CDS             1593..1862
                     /gene="rpsO"
                     /locus_tag="VF874_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9175692.1"
                     /translation="MALTAEQKKEILAQYGLHETDTGSPEAQIALLTKRINDLTEHLK
                     VHKHDHHSRRGLLLLVGRRRRLGKYLSEVDVERYRSLIDRLGMRR"
     gene            complement(2192..>2376)
                     /locus_tag="VF874_06210"
     CDS             complement(2192..>2376)
                     /locus_tag="VF874_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175693.1"
                     /translation="SATDMTVRIFLRSSCRRYAFAVASNLNGIGLNAATAVDRSGRIS
                     VRQPLPKTARAGWPLS"
BASE COUNT          413 a          807 c          786 g          370 t
ORIGIN      
        1 acgctgcgcc gcgccggcga acggttcggc ttcgcggtgc gcgccatgtc gctggtcgcc
       61 gagcaccatg acgacgaatc ggtggcgttc tcgtccacct acatccggtc gtgcgtcgac
      121 gcaggggacg tggtggcggc caccgaagcg ctgggccggc cgcaccgggt cgagggtgtg
      181 gtggtccgcg gcgaaggccg cggccgggtg ctcggcttcc cgaccgccaa cgtcgctccg
      241 ccgatgtatt ccgcgatccc ggccgacggc gtgtacgcgg cctggttcac cgtgctgggc
      301 cacggacccg tcgtcggcac cgtcgcgccg ggcgagcggt atcaggccgc cgtgtccgtc
      361 ggcaccaatc cgacgttctc cggacgcacg cgcaccgtcg aggcgtacgt gctcgacacg
      421 tccgccgacc tgtacggcca gcacgtcgcg atcgacttcg tcgcccgcat ccgcgggcag
      481 cgcaagttcg ccgaggtcgg cgacctgatc gccgagatgg aggccgacac caagcgggcc
      541 cgcacgattc tgctggcccg ctagcccgcg agggcttggc cgcctctgaa ccgcctgagg
      601 aaggagatca gccgataggc gggcaccgcc ttcggccagt cattggcgcg gaagtaggca
      661 tccgagccgc cgtcgacgaa cacgacgctg ccacacaaga agtccgcggc atccgacagc
      721 atgaagcacg cccagtcggc caactgccca gcatcgccga acccgccgat cggaatcgga
      781 aacgaccgaa ccgccttggc ctccttggga gtcgacaact gctcctgcag aagcggtgtc
      841 atcaccgcgc ccggcgcgag agcgttgagg cggattcccg cgccggccca ctccggggtc
      901 accgcgttgc gtcgaaccca tcggctgacc gcgattttcg acgcggcgta catcatcacc
      961 gaggcgttct tgccgaacca tcgcacggcg cgcaccgcct tgtcggcgtc atgcgtcagc
     1021 aatgcgcgga cggcgcggct cggcaccgcg ggcacggtgg ttgacgaatt actggaaatg
     1081 acaacgactt tggcgcgctc agtggaggca agcgcggtcc gccagctagc gagcaggtcg
     1141 acgacgccca ggtagttgac ttgggcgatc tggcgcgggc ggtcggagcc gggggcgggt
     1201 ccaaggccgg ccgccagcat cgccccgtcg agtccggcgc ctgacgccgc gagcactctg
     1261 tcggcggcgg tcttgcgtcc ggcaggtgtc gacaagtcgg cgaccacctc ggcacttctg
     1321 atgtcgaccc cgatcacggt gtggccgccg tcacgtaatc gctgggccgt ctcacggccc
     1381 atgcccgacg ctgatccggt gacggcatag gtgcccacgg gagtcctttc tcgcgcacaa
     1441 cgctggttgc gttgaagcta cgcggccgac ggcatcgatt cgggccgcac accacatcgc
     1501 tgctagactc gccgccgaca aggcgacgcg ctgcagtccg cggcggccgc gcccaaattc
     1561 cctgatcgcg gaccgaagat ggagttgaat tcgtggcgct cactgccgaa cagaagaaag
     1621 aaatcctggc tcagtacggc ctgcacgaga ccgacaccgg ttcgccagag gcgcagatcg
     1681 cgctgctcac caagcgcatc aacgacctca ccgagcacct caaggtgcac aagcacgacc
     1741 accactcgcg gcgcggactg ctgctgctgg tggggcggcg ccgtcgcctg ggcaagtacc
     1801 tgtccgaggt cgacgtggag cggtaccggt cgctcatcga ccgcctcggc atgcggcgct
     1861 gacccgtctg gcctgcgcgc cacacgtgtc acaggcgtct ccctgaaagt ggaggtgctc
     1921 tttgcccacc ttcgagcgac aacggcgttt tcccagttca gcggccacaa cgacgatggg
     1981 acaccagcga caataggtgg cgtttatgca cgtcagaagt tgtcgctcga aggcgggcac
     2041 atcgacaccc cggatactcg gagactggtg agactggtgt ggctaccgtg ccgatccacg
     2101 aagccgacgc tctcggcgcg agtaaaggct tgcgaacggc gccggtgtag agtaaaggca
     2161 ttccgagcca acccggctcg gatgtcgcat gtcacgacag tggccagcct gcacgggcgg
     2221 tcttcggtag tggctgccgg actgaaatcc ggccgcttcg atcgacggcc gtagccgcgt
     2281 tcaggccgat gccgttcagg ttgctcgcca ctgcaaaggc atagcgacga caacttgaac
     2341 gaaggaaaat ccggactgtc atgtctgtag ctgaaa
//
LOCUS       DASWBF010000178         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_675137,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000178 DASWBF010000000
VERSION     DASWBF010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675137"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..318)
                     /locus_tag="VF874_06215"
                     /pseudo
     CDS             complement(1..318)
                     /locus_tag="VF874_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167116.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            951..1328
                     /locus_tag="VF874_06220"
     CDS             951..1328
                     /locus_tag="VF874_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175694.1"
                     /translation="MAVKWLDEPEAHDYGAAAEYLSMVAEADAVAKAVAALREAKPIY
                     RKAKDILRAAGLALLPETNPHVKSDLEKISDGKKLSPILLVRGDAARSVRLGIADGYH
                     RVCASYVTDENTAIPCRLVSWST"
     gene            1336..2577
                     /locus_tag="VF874_06225"
     CDS             1336..2577
                     /locus_tag="VF874_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9175695.1"
                     /translation="MSGFGFDTLALLAVIGLAGPLLAAVPRFGIPVVIGELVVGLIVG
                     RTGFGVVDHTDPTFTLLANIGFALVMFVVGTHVPVRDTTLRSAIPKALLRAVVVGAVA
                     AGLGVTLAEAFGTGHAALYAVLMASSSAALALPIIQSLGLGGPQVLSVTAQIAIADAT
                     SIVLLPLVIDSRRTPQAALGALAIAACAAVLFLVFRAADRRLLRKRLHHYSEKHRFAL
                     ELRISLIVLYSLAALAIVTHVSIMLAGFALGLVVSAIGEPRRLARQLFGITEGFFSPL
                     FFVWLGASLQVRELGDRPGFILLGAGLGVGAVLAHCAGRLLGQPLTLAALAAAQLGVP
                     VAAATLGTEQHLLAPGEPSALMLGALLTIAVTSVAGVLAARRHSGDTSGDTTSAENVL
                     DAPSTGRRPGGGHRRGEGRPA"
BASE COUNT          405 a          856 c          908 g          485 t
ORIGIN      
        1 gcccgatcag cagcgctcgc agccaccttt ccactgcgag agcgcgaacg atgatggctg
       61 gcgcaacgct tttccccgaa gcactatcgg cgcttggtca gcgggcccgc ggccgtgcgg
      121 cgctcccatg acgaattcac cgcagcgcag gcagcgccac acttggccca gtccggtggt
      181 tccgctgagc cgctcagcga gcgcgacgtc atcaggcgcg taggtgacgt ggcctcggcg
      241 cgcgcacgtg agcagctcaa agcggttgcc gcccgcagat cctcgaacag cggttcggcg
      301 cttcttcgat gccgccacga ggcgatcatc gcctgccggc ggcagctgac gggtcaatat
      361 cgccgtagcg atgcctgaac gttttctccc cggtcgcgcc gaaatgagtc agactgctgg
      421 tcccggccac gactcgcata cgagccatcc tggaggttcg tcgacacctg tgcccggaca
      481 caccattgcc ctgccgcaca acgactctga tctgcgtgcg catatttcgc gtcggagagc
      541 gctcgatgcc gcacggaggc gctcggcgat ttcggccagg cttcagacgg tgaccgtgcg
      601 gacagtgttg cggtagccgg accatccagg cacccgacat cgaacgcacc aattcaccga
      661 cgacagtcat tgggcctcaa tagttcacgc aattcggtgc tgaggtgtgt cgtgaacgtg
      721 aaaggtgcac tgcctcaacg ataatcgacg tttgctggcg ttcgagaatc gatcgaggag
      781 tgcgttggga caaaacccag tgaaatcgat gtgaacaagc gtgacgaggc gaaacggctt
      841 ggacggctga atgccgctca cctggagcta tgagccccga ccgtactgcg agagaccgcg
      901 actggttcgt tgacaaccca ctgacagcca tccggattat ggtgtcccgg atggctgtca
      961 agtggctgga cgagccggag gcgcacgact acggcgccgc ggctgagtac ctgagcatgg
     1021 tcgccgaagc cgacgcggtc gccaaggcgg tcgcggcgtt gcgggaggca aagccgatct
     1081 accgcaaggc caaggacatc ctgcgggccg cgggcctggc attgctgccg gagaccaacc
     1141 cgcatgtgaa gagtgacctc gagaagatca gcgacggcaa gaagctctcg ccgatcctgc
     1201 tggtgcgtgg cgatgcggcc aggtcggtgc ggctggggat cgccgacggc taccaccggg
     1261 tgtgcgcgag ctacgtgacc gacgagaaca ccgcgattcc gtgccgcttg gtgtcgtggt
     1321 cgacctgatg gtctgatgtc cgggttcggt ttcgacacgc tggccctgct cgccgtcatc
     1381 ggcctggcgg gcccgctgct ggccgcggtg ccgcggttcg gcattcccgt ggtgatcggc
     1441 gagttggtcg tcgggctcat cgtcggcaga acaggtttcg gggtcgtcga ccacaccgat
     1501 ccaacgttca cgctgctggc caacatcggc ttcgcgctgg tgatgttcgt cgtcggcacc
     1561 cacgtacccg tacgcgatac gacgctgcga tcagcgatcc cgaaggcgtt gctgcgtgcg
     1621 gttgtcgtcg gcgctgtggc ggccgggctg ggggtgacgc tcgccgaggc cttcgggacg
     1681 ggacacgcgg ccctgtacgc cgtgctgatg gcgtcgtcgt cggccgcttt ggcgctgccg
     1741 atcatccaat cgctgggcct gggcggaccg caggtgctgt cggtgaccgc gcagatcgcc
     1801 atcgctgacg ctacctcaat tgtgttgctg cccttggtga tcgactcccg gcgcacgcca
     1861 caggcggcgt tgggtgcgct ggccatcgcg gcctgcgcgg cggtgctttt cctggtcttc
     1921 cgggcggccg atcggcggct tctgcggaag cgcctgcacc actactctga aaagcatcgg
     1981 ttcgcgctgg aactgcggat cagcctcatc gtgctgtaca gccttgcggc gctggccatc
     2041 gtcacgcacg tgtcgatcat gctggcgggt ttcgcgctcg gtctggtggt gtcggcgatc
     2101 ggtgaacccc gtcggctcgc gcgtcagctg ttcggcatca cggaggggtt cttcagcccg
     2161 ttgttcttcg tctggctggg cgcctccctg caggtgcgcg aactcggtga tcgcccgggg
     2221 ttcatcctgc tcggggcggg ccttggcgtc ggtgcggtgc tggcccactg tgcgggcagg
     2281 ttgctcggcc agcctctgac ccttgcggcc ctggccgcag cccaactggg cgtgccggtg
     2341 gcggcggcga ccctgggcac cgaacagcat ctgctcgcgc ccggcgaacc gtcggcgctg
     2401 atgctcggcg cgctgctgac gatcgcggtc acctccgtcg cgggagtgct ggccgcgcgg
     2461 cgacatagcg gcgacacatc gggcgacacg actagtgcag agaatgttct tgacgcgcca
     2521 tcgactggcc gccgcccggg cggcggccac cgtcgtggtg aaggccgtcc tgcgtagtgg
     2581 cgcgtctgag taaccgtcgg gccgccgcaa acatgcaagg ccgggaaaac ccgccgtctt
     2641 gtcggcatcg cggc
//
LOCUS       DASWBF010000179         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_78599, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000179 DASWBF010000000
VERSION     DASWBF010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78599"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..491
                     /gene="tatB"
                     /locus_tag="VF874_06230"
     CDS             51..491
                     /gene="tatB"
                     /locus_tag="VF874_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX9175696.1"
                     /translation="MFENLGWSHLMVLGLVGLIVLGPERLPGAIRWAVSALRQARDYI
                     SGATSQLRDELGPEFDELREPIGELQKLRGMTPRAVLTQHLLDGDDSLFTAMCDLRAE
                     PASRHVGDRADVASVLPTGPNPVGPSTGGAERPGEATPFDRDST"
     gene            complement(605..952)
                     /locus_tag="VF874_06235"
     CDS             complement(605..952)
                     /locus_tag="VF874_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9175697.1"
                     /translation="MLGADADLRASALTPGQPHVTAAAPVHLIYVADLHRLTHTMGFD
                     EPGLHDPEVQKSYYYYVDTGLIAGNVYLFAAARGLAAWFHNCDRAALSQRLGLSAQQR
                     VLFAQSVGYPAAR"
     gene            complement(1019..1252)
                     /locus_tag="VF874_06240"
     CDS             complement(1019..1252)
                     /locus_tag="VF874_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175698.1"
                     /translation="MADETQADRVLQLPAPAVDHDDPILTTLQRRQTIREISATPLPL
                     QELSDLLWSACGVNRELGPFGAPGRTAGSASNS"
     gene            complement(1341..1949)
                     /locus_tag="VF874_06245"
     CDS             complement(1341..1949)
                     /locus_tag="VF874_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HEX9175699.1"
                     /translation="MNLKKVVAAMSVASGLGLVFGGAGSGVAIAAPCPPGGVAGAQCG
                     GPGPGPQGGPPSAPQAGAAPPGRQEPPTDGAGNFRGLDGQQPDLPRRRPSDSPRSDFP
                     GSGLGNRGGPPNGPDWGQVPRQDWGRPDQDDWRRRPPPWGDGPAPWGWGPPPRPEYSG
                     PLPPPWGPPPPPINYWGFSCQPVWDAGFNQWGIWFFGIWIPL"
     gene            complement(2250..3230)
                     /locus_tag="VF874_06250"
     CDS             complement(2250..3230)
                     /locus_tag="VF874_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9175700.1"
                     /translation="MGLTINGWLGYFPTVATAVNQLTGGPLPDQTDMATVRAMQSRGA
                     QPVTGALVPVDIDSAASGFRHRPEWVYLPPAWFTAKPASRLPTLMMIGAEFNTPADWV
                     RAGDAVHTLDVFAAGHNGEAPVAVFVDPTGAFNNDTECVNGPRGNAADHLTKDVVPYT
                     VATFGLSGDRSRWGVVGFTSGGTCAVDLAVMHSDMLSAFVDIAGDRGPHAGTKAQTVD
                     RLYGGDAEAWSAFDPTTVITRHGPYADLSGVFAVPAAVGARSAGNAYDARSRGQADAA
                     ETQCALGRSKAISCSVQSYPGGHTWPFAATAFRAALPWLADRLGRPDVAP"
     gene            3229..3396
                     /locus_tag="VF874_06255"
     CDS             3229..3396
                     /locus_tag="VF874_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175701.1"
                     /translation="MLSKQNEAAKTETLRRHHPALGQPTTVTAAADPLSETLSHICHG
                     GGSLAMPNVPK"
     gene            3485..>3585
                     /locus_tag="VF874_06260"
     CDS             3485..>3585
                     /locus_tag="VF874_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEX9175702.1"
                     /translation="MGGVSTATASYKGHRYPVEIINHCVWLYFRFPL"
BASE COUNT          607 a         1182 c         1220 g          576 t
ORIGIN      
        1 ctcaacgcct acgccacccg ggtcgactaa caacagtgca gaataggtag atgtttgaga
       61 acctcgggtg gagtcatctg atggtcctgg gcctggtcgg tttgatagtc ctgggtccgg
      121 agcggctgcc cggcgcgatc cggtgggcgg tctccgcgct gcgccaagcc cgcgattaca
      181 tcagcggtgc caccagtcaa ctccgcgatg aactgggacc cgaattcgac gaactgcgcg
      241 agccgatcgg cgagctgcaa aagttgcgcg gaatgacgcc gcgcgcggtg ctgacccagc
      301 acctgttgga cggcgatgac tcgttgttca ccgcgatgtg cgacctgcgc gccgagccgg
      361 catcacggca cgtcggtgac cgcgccgacg tcgcctccgt cctaccgacc ggacccaacc
      421 ctgtcgggcc ttcgaccggc ggggcggagc ggcccggcga agccacaccg ttcgaccgcg
      481 actcgactta gcgatgcctg gaccccttct gcccggccgc gccaaagtgg acaggaccgc
      541 tgggtgcctc gtctaccaac aactttgcgg ggcgtgtgat cgccggataa cgcgggccac
      601 cggctcaccg cgccgcgggg tagccgacgg attgagcaaa cagcacccgc tgctgtgcgc
      661 tcaggccaag gcgctggctc agcgcggccc gatcacagtt gtgaaaccac gccgcgagcc
      721 cacgagccgc ggcgaagaga tagacattgc cggcgatcag cccggtgtcg acgtagtagt
      781 agtaggactt ctgcacctcc gggtcatgaa gccccggctc atcgaatccc atggtgtggg
      841 tgagccgatg aaggtcggcg acgtaaatca ggtgaacggg agccgcggcc gtgacgtgcg
      901 gttggcccgg cgtcagcgcg ctagcgcgca agtcggcgtc ggctcctagc accaggcgac
      961 tgttcacagc gtcatagcga tacgttgccc ccgccaaggc gacgtagacg tcgatctctt
     1021 acgaattgct ggccgaaccg gccgtgcggc ccggcgcccc aaaagggcca agctcgcggt
     1081 tcacaccgca cgccgaccac agcaggtcgg aaagctcctg cagcggcaac ggggtggcgc
     1141 tgatctcgcg gatcgtctgt cgtcgctgca gcgtggtgag gatcggatcg tcgtggtcga
     1201 ctgccggcgc aggcagctgc aggacccggt cggcttgcgt ctcatcagcc actgtctgct
     1261 gcccgatcga ttcgtcacgt tacgtcagcg gtcgacgtca tgcgacggac atcctatgca
     1321 tctgctgatc cttcagtccg tcacagtgga atccagatac cgaagaacca gatgccccac
     1381 tggttgaagc ccgcatccca gaccggttgg catgagaatc cccagtagtt aatcggcggc
     1441 ggtggcggac cccacggcgg cggcagcggg ccggaatact cggggcgtgg gggcggaccc
     1501 cagccccacg gagcgggtcc gtcgccccac ggcggcgggc ggcgccgcca atcgtcttga
     1561 tctggtctgc cccagtcctg tcggggcact tgcccccagt ccggtccgtt aggcggtccg
     1621 ccgcggtttc caaggccgga cccgggaaag tcggatcggg ggctatccga cgggcggcgg
     1681 cggggcaggt ccggttgctg tccgtcgagc ccgcggaagt tcccggcgcc gtcggtaggc
     1741 ggctcttgcc gaccgggggg cgccgcgccc gcttgtggag cactcggcgg gccgccctgc
     1801 ggcccgggac ccggcccacc acattgtgcg ccggcaaccc ctccgggcgg gcaaggtgcc
     1861 gcgatcgcga cgccggaacc ggcgccgccg aagaccagtc ccaatccgct ggccaccgac
     1921 atcgcagcaa ccactttctt gaggttcatc acgtaactcc ttgttctcaa aggtgtttga
     1981 ctcgatcaac ccccgactta agcaaggatg gccagccggc ctatgagcca gctatgtctg
     2041 gtgtgcgacc agcctggcaa tcgctgtcag ttgggcagag tgaccgtctg ctcttcggcc
     2101 gcggcatgcg gttgagcatg cactccggcg acaaccctcc ttctgcttcc accgtccagt
     2161 tggctgcagg aacgacgcac ggtacagccg tcgcagactt cagtccggtt gtcgaaggcg
     2221 tcggtcgagc gttatggcac ggcagcggtt cagggtgcga cgtcgggcct gccgagtcgg
     2281 tcggcgagcc agggcaacgc cgcgcggaat gcggtggcgg cgaagggcca cgtatgcccg
     2341 cccggatagc tctgcaccga gcagctgatg gccttggatc tgcccagggc acactgcgtt
     2401 tcagcggcgt cggcctggcc ccgggatctg gcgtcgtagg cgttgccggc ggagcgggcg
     2461 ccgacggctg ccggaacggc gaacacaccc gagaggtcgg cgtagggacc gtgtctggtg
     2521 atgacggtgg tcggatcaaa cgccgaccat gcttcggcgt cgccgccgta cagccggtcg
     2581 acggtctggg ccttggtgcc cgcgtgcggg ccgcgatcac cggcgatgtc gacgaacgcg
     2641 ctcaacatgt cggaatgcat cacggcgagg tccaccgcgc aggtgccgcc cgaggtgaat
     2701 ccgactacgc cccaacggct tcggtcgccg ctcaaaccga aagtggcaac cgtgtagggg
     2761 acgacgtcct tggtgaggtg atcggcggcg ttaccgcggg gcccgttcac gcactcggtg
     2821 tcgttgttga aagcgccggt cgggtcgacg aagacggcta ccggagcttc gccgttgtgc
     2881 ccggcggcga acacgtcgag ggtgtgcacc gcgtcgccgg cgcgaaccca gtcggccggg
     2941 gtgttgaact ctgctccgat catcatgagc gtcggcagcc gggatgcagg cttggcggtg
     3001 aaccaggcag gcggcagata cacccattcc ggacggtgcc tgaaaccgga tgcagcagag
     3061 tcgatgtcga ccggcacgag ggctccggtg accggctgag ctcctctgga ctgcatggct
     3121 ctaacggttg ccatgtcggt ctggtctggc aggggcccgc cggtcagctg attgaccgcg
     3181 gtagcgacgg tcgggaagta gcccagccac ccgttgatgg tcagccccat gctgagcaag
     3241 cagaacgaag cggcgaagac ggagacgttg cgccgccacc acccggcgct gggccagccg
     3301 actacggtga ccgcagcggc cgacccgctg agcgaaaccc tcagccacat ctgccacggc
     3361 ggcggctcac tggcgatgcc caacgtcccg aagtagcaat gcgccgcaaa tccactgacc
     3421 acgccgacca ccacggcaac cggcagggca ctgtcacgtt ggtgagtgtc ggcgccctgg
     3481 aaggatcggt ggggtgagca ccgcaacggc gtcgtacaag ggccaccgct acccggtcga
     3541 gatcatcaac cattgcgtgt ggctgtactt tcggttcccg ctgag
//
LOCUS       DASWBF010000180         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_346925,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000180 DASWBF010000000
VERSION     DASWBF010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346925"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VF874_06265"
     CDS             <1..713
                     /locus_tag="VF874_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX9175703.1"
                     /translation="QPYPYVVAIVDELADLMMTAPRDVEDAVVRITPKARAAGIHLVL
                     ATQRPSVDVVTGLIKTNVPSRLAFATSSLIDSRVILDQPGAEKLIGMGDGLFIPMGAN
                     KAIRLQGAYITDEEIHAVVAACKEQAEPQYTDGVTSAKPTGERTDVDPDIGDDMDVFL
                     QAVELVVSSQFGSTSMLQRKLRVGFAKAGRLMDLMETRGIVGPSEGSKAREVLVKPDE
                     LAATLASIRGLDGAADDD"
     gene            complement(710..964)
                     /locus_tag="VF874_06270"
     CDS             complement(710..964)
                     /locus_tag="VF874_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175704.1"
                     /translation="MFHRTAGWLVAATLGFAATLITPVPAQAAPDCSDDSSVTGYCPT
                     GTCREIQVNESGVQCLDNALDPLLPPAPPVRVEIGVGAGS"
     gene            complement(981..1493)
                     /locus_tag="VF874_06275"
     CDS             complement(981..1493)
                     /locus_tag="VF874_06275"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX9175705.1"
                     /translation="MSAARSDGLVIRRARTSDVPTIKSLVDYYAGKILLEKNLVTLYE
                     AVQEFWVAELDGEVIGCGALHVMWSDLGEVRTVAVHPKVKSHGIGHAIVDRLIEVARE
                     LQLQRAFVLTFEIEFFSRHGFVEIEGTPVTAEVYEEMCRSYDTGVAEFLDLSYVKPNI
                     LGNSRMLLML"
     gene            1536..2144
                     /gene="pgsA"
                     /locus_tag="VF874_06280"
     CDS             1536..2144
                     /gene="pgsA"
                     /locus_tag="VF874_06280"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX9175706.1"
                     /translation="MPAPPSRDPAAAPTSPTARLLNVANVLTTIRLVLVPVFLFALFA
                     DGGYETRWRIAAFVVFAVAMVTDRFDGALARSYGMVTEFGKLADPIADKALLGAALIG
                     LSLLHELPWWVTVLILVREIGITVLRFAVLHRGVIPASRGGKLKTLVQAIAIGLFILP
                     LSGAWHVVAMAVMAVAIVLTVVTGIDYVAQAVKDSHGRPADQ"
     gene            2226..2549
                     /gene="clgR"
                     /locus_tag="VF874_06285"
     CDS             2226..2549
                     /gene="clgR"
                     /locus_tag="VF874_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ClgR"
                     /protein_id="HEX9175707.1"
                     /translation="MAALLREVIGDVLRRARTSQGRTLREVSDSARVSLGYLSEVERG
                     RKEASSELLTAICGALEVPLSELLTDAGRQLQRQERGDGATSAANIDAATKVVIPPVV
                     SMAVA"
BASE COUNT          449 a          847 c          870 g          446 t
ORIGIN      
        1 accagccgta cccgtacgtg gtggcgatcg tggacgagct cgccgacctg atgatgaccg
       61 cgccgcgcga cgtcgaggac gcggtggtgc ggatcacccc gaaggctcgc gcggccggca
      121 tccacctggt gctggccacc cagcgtccgt cggtggacgt ggttaccggc ctgatcaaga
      181 cgaacgtgcc gtcgcgactg gcgttcgcca cgtcatcgct gatcgacagt cgcgtcatcc
      241 tggatcagcc gggcgccgag aagttgatcg gcatgggcga cgggctcttc atcccgatgg
      301 gcgccaacaa ggcgatccgc ctgcagggcg cctacatcac cgacgaggag atccatgccg
      361 tcgtcgctgc ctgcaaggaa caggccgaac cgcagtacac cgacggtgtc accagcgcca
      421 agccgaccgg agagcgcacc gacgtcgacc ccgatatcgg cgacgacatg gacgtcttcc
      481 tgcaggccgt ggagttggtg gtgtccagcc agttcggttc gacgtcgatg ctgcagcgca
      541 agctgcgggt cggattcgcc aaggcgggcc ggctgatgga tctgatggaa acccgcggca
      601 tcgtcgggcc gagcgagggc tccaaggcac gcgaggtgct ggtcaagccc gacgaactgg
      661 cggcgacgct cgcgtcgatc cgcggcctcg acggcgccgc tgacgacgac tagcttccgg
      721 cgccgacgcc gatctcgact cgcaccggtg gcgccggggg aagcagcggg tccagtgcat
      781 tgtccaggca ttgcacaccc gactcgttca cctggatctc gcgacaagtc ccggtgggac
      841 agtagccggt gaccgacgaa tcgtcggagc agtccggcgc cgcctgagcc ggtaccggcg
      901 taatcagcgt cgcggcgaat cccagagttg ccgcgaccaa ccacccggcc gtgcgatgaa
      961 acacacgtcc acggtacgac tcacagcatc aacagcattc gcgaatttcc gagaatgttg
     1021 ggcttgacgt aggacaggtc cagaaactcc gccacaccgg tgtcgtagga gcggcacatc
     1081 tcctcgtaaa cctccgcggt gaccggggtg ccctcgatct cgacgaagcc gtgtctgctg
     1141 aagaactcga tctcgaaggt gagcacgaac gcgcgttgaa gctggagttc gcgtgccact
     1201 tcaatcagcc ggtcgacgat cgcatggccg atgccgtggc tcttcacctt cggatgcact
     1261 gcgacggtgc gtacctcgcc gaggtccgac cacatcacat gcaaagcgcc gcagccgatc
     1321 acctcgccgt cgagttcggc gacccagaac tcctggaccg cttcgtagag cgtcacgagg
     1381 ttcttctcca gcagaatctt gccggcgtag tagtcgacaa ggctcttgat ggtcggcacg
     1441 tccgaggttc gggcacgacg gatcaccagg ccgtcggacc gtgctgcgct cacacaccga
     1501 acatatcggc tgggcgcaac agatattctg atgccgtgcc agcgcccccc tcacgcgacc
     1561 cggcggcggc gccgacgtcc ccgacagcac ggctgctcaa cgtcgcaaat gtcttgacca
     1621 ccatccggct ggtgctggtg ccggtcttcc tgttcgcgtt gttcgctgac ggtggctacg
     1681 aaacccgttg gcgcatcgcg gctttcgtcg tctttgcggt tgccatggta accgaccggt
     1741 tcgacggcgc gttggcccgc agctacggga tggtcaccga attcggcaag ctggccgacc
     1801 cgatcgccga caaggcgctg ttgggcgcgg cgctgatcgg gctgtctttg ttgcacgagc
     1861 tgccgtggtg ggtgacggtg ctgatcctgg ttcgtgagat cgggatcacg gtgctgcggt
     1921 tcgccgtgct gcaccgcggc gtgattccgg caagccgcgg cgggaagttg aagacgctgg
     1981 ttcaggcgat cgcgatcggg ctgttcatcc tgccgctgtc gggggcctgg cacgtcgtcg
     2041 cgatggcggt gatggccgtc gcgatcgtgc tcaccgtcgt caccggaatc gactacgtcg
     2101 cacaggccgt caaggacagc cacgggcgcc ccgccgatca gtgacgggaa ccatccgacc
     2161 ggcggccgac gttgccactt cagtaggcgt ttcagtaggt atccgcccac ccgaaggagt
     2221 gcacgatggc agcgttgctg cgcgaggtga tcggcgacgt gcttcgtcgc gcccgcacgt
     2281 cgcaggggcg gaccctgcgc gaggtgtccg actcggcgcg cgtgagcctg ggatatctgt
     2341 ccgaggtgga gcgtggccgc aaagaggcgt cgagtgaact gttgaccgcg atctgcggtg
     2401 cgctggaagt tccgctgtcc gagctgctga cggacgctgg ccgccagctg caacgtcagg
     2461 agcgcggcga cggggccacc agcgcggcca acatcgatgc ggccaccaag gtcgtcatcc
     2521 cgcccgtcgt gtcgatggcg gtggcctgac ccactgcgcc gcatggccct gaacggataa
     2581 attgggcata cgcgcactta atccggcagt aa
//
LOCUS       DASWBF010000181        33645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_17795, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000181 DASWBF010000000
VERSION     DASWBF010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 33645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33645
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17795"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF874_06290"
     CDS             complement(<1..217)
                     /locus_tag="VF874_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9175708.1"
                     /translation="MSHGQLPGVFGDVQPVLEHYGYVAVGGFVLLEDFGVPVPGEAIL
                     ITAAIFAGTGHMNIALVIVVAVLGAVIG"
     gene            431..1456
                     /locus_tag="VF874_06295"
     CDS             431..1456
                     /locus_tag="VF874_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX9175709.1"
                     /translation="MNVSQLEWIITLGATIAVLLFDVMIIGRRPHKPSMRECAIYLTI
                     YVGLAVAFGVFVWFFHGSQFGLEFFAGWLTEYSLSVDNLFLFLLIMASFNVPEKYQQQ
                     TLLVGIVLALVFRGIFIALGAVAINEFSWVFYLFGAFLIYTGFHLARDKDHDDDADNR
                     VVRFAKTYLNTTDRWDGLKLYVKEGSKRLMTPMFLVILALGTTDIMFALDSIPAIYGL
                     TSEPYLVFTANVFALMGLRQLYFLLGDLLKRLVYLSQGLAVILCFIGVKLTLHALHEN
                     EVPFINGGKHVDVPEIPTLFSLGVIVATLIVTTVASLVKTRVIDANSDDDDAVAEEGP
                     AESERTE"
     gene            complement(1741..2118)
                     /locus_tag="VF874_06300"
     CDS             complement(1741..2118)
                     /locus_tag="VF874_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175710.1"
                     /translation="MVVDEEHHQPGILSAISLGGNVNGVQLLRRETVEKIFEVQCDGP
                     DLVLAQPPVRWGVGYGLPQRDTFPYVPDGKICFWGGWGGSWELMNPDHRATFAHVMNK
                     MGPGIEGSERTARYARLFYKTLG"
     gene            complement(2339..2854)
                     /locus_tag="VF874_06305"
     CDS             complement(2339..2854)
                     /locus_tag="VF874_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX9175711.1"
                     /translation="MHQGIGLDAFNELSPQKAMHALYECCNSVVLARDLAGGRPYASH
                     DELLRRADALLFALSESSVDTILQAYPHIGRRPRSTKSQAEQCSVWDPRPEVMAQLDT
                     AAQRYTEQFGLSFVMFVDGTDAEGVLAAISDRLHHEIDTERKVVRNELAKINRTRLER
                     MLGPEGGYHNW"
     gene            complement(2995..3483)
                     /locus_tag="VF874_06310"
     CDS             complement(2995..3483)
                     /locus_tag="VF874_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX9175712.1"
                     /translation="MQFDVTIEIPKGQRNKYEVDHETGRLRLDRYLYTAMAYPTDYGF
                     IEDTLGEDGDPLDALVLLPESVFPGVIVEARPVGMFRMTDEAGGDDKVLCVPAGDYRW
                     DHVKDIGDVPSFELDAIKHFFVHYKDLEPGKYVEAADWVGREDAEAEVLRSLERFQAG
                     GH"
     gene            3548..4930
                     /gene="dacB"
                     /locus_tag="VF874_06315"
     CDS             3548..4930
                     /gene="dacB"
                     /locus_tag="VF874_06315"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX9175713.1"
                     /translation="MHPARWRRSTHFLIGAAVLVVVVVAAAALLGNGGSSRSAKAAPA
                     TPPAAAAKLSVVPVVDAAPLPTPAGLAAALKQPLANPDLGALTGRVTDALTGTELWQQ
                     GGTTPMVPASTNKVLTAGAALLTLNRDTRQTTTVVAPDQSRQPGVVVLVGGGDPTLSS
                     APPGEETLYRNAARISDLAAQVRGSGIKVTAIQVDASAFSGPSLAPGWDPVDILGGDV
                     APIEAVMVDGGRTQPLSPESVRSFTPALDAGQALAAALNIDPQAVTMAAVPMRDGRRI
                     ASVQSAPLMDRLNEMMNHSDNVMAECIAHEVAVAVGRPASFGDAVQAVTSRLAAAGVD
                     MTGSRLMDGSGLSVDDRLTARTLDGVIGAAAGVSAPAMRPLLDVLPIAGGSGTLSNRY
                     IDDQTDEAAGWLRAKTGSLTGTNTLAGVVTDVSGRVLTFTLMSNNAGPTGRTAIDALA
                     ATLRSCGCGS"
     gene            4918..5982
                     /locus_tag="VF874_06320"
     CDS             4918..5982
                     /locus_tag="VF874_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX9175714.1"
                     /translation="MWLVTPTQDSRKLTVGRVVDWDFAATTGARLARPGPPSSDYTQR
                     QIVDELAESSRRAEVPVREVTGLVPGGEVPDALIVDRPHWVRLAASSMRVMTGGDDEP
                     RGFLRGRITGAQTGAVLAFVASGILGQYDPFAASGGKLLLIYPNIIGVERQLQVVPRD
                     FRLWVCLHEVTHRVQFTANPWLADYMSSALGVITEDAADDFGNVVGRLADYVRDRRDG
                     APAEPNSNGIIGLLRAMQSEPQRQALDQLLVLGTLLEGHADHVMDAVGPAVVPTVTTI
                     RERFDERRRRRQPPLQRLIRALLGFDAKISQYTRGKAFVDAVVSRVGMERFNAVWSGP
                     DTLPLPDEIDKPERWIARVL"
     gene            5961..6923
                     /gene="tilS"
                     /locus_tag="VF874_06325"
     CDS             5961..6923
                     /gene="tilS"
                     /locus_tag="VF874_06325"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX9175715.1"
                     /translation="MDRTGAVAALGEAVSAFTLRYSISEKPWCVAFSGGPDSLALTAV
                     CVGMRPTTALIVDHGLQDGSAAVADTARRQALDLGCVEAQVLRVDVGRLGGPEAAART
                     ARYAALQEASAGAPVLLGHTLDDQAETVLLGLGRGSGARSLAGMRPYQPPWCRPLLGV
                     RRAVTHAACDELGLSAWQDPHNSDPRYTRSRLRRDVVPLLEDVLGGGVAEALARTAAA
                     LREDCDALDDLARTELDAVVTDQGLDARQLNDLPDALRRRVIRGWLLAGGATDLTDNQ
                     IRRVDELVMNWHGQGGVAVGSALRSQRLVAARRNGRLRLHREPV"
     gene            6992..7552
                     /gene="hpt"
                     /locus_tag="VF874_06330"
     CDS             6992..7552
                     /gene="hpt"
                     /locus_tag="VF874_06330"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX9175716.1"
                     /translation="MYSGDIKSVLLTESQIQNRTAELGAAIGERYRGLIDSSGDDLLL
                     VTVLKGAVMFVSDLARAIPLPTQLEFMAVSSYGSATSSSGVVRILKDLDRDIHDRDVL
                     IVEDIVDSGLTLSWLLRNLAARHPRSLRVCTLLRKPDAVRSDVGIDFVGFDIPNEFVV
                     GYGLDYAERYRDLPYIGTLDPRIYQE"
     gene            complement(7574..8230)
                     /locus_tag="VF874_06335"
     CDS             complement(7574..8230)
                     /locus_tag="VF874_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782514.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX9175717.1"
                     /translation="MSACDEQSDSSPQAGPNNRQVTVIGAGQVQGTPDTLTANVSIEA
                     TAPDVTTAMNQTSGRTQAVIDALVNDAKVDRKDISTTSVSLQPQYGSDSSAIIGYRSS
                     NSLAVKIRDLGSASRTLALIVSTGGDATRIDSVNYSIDDDSQLVRDARARAFDDAKAR
                     AEQYAELSGLRLGKVISISEASGATPPTPMPRAATEAVPLEPGQQTVSFSVTVIWELT
                     "
     gene            complement(8313..9506)
                     /locus_tag="VF874_06340"
     CDS             complement(8313..9506)
                     /locus_tag="VF874_06340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9175718.1"
                     /translation="MEGPLRFGVFITPFHPVGQSPTVALEYDLERVVALDRLGYDEAW
                     FGEHHSGGYELIACPEVFIAAAAERTKHIRLGTGVVSLPYHHPLMVADRWVLLDHLTR
                     GRTMFGTGPGALPSDAYMMGIDPVEQRRMMHESLEAILALFRAAPGERIDRHSDWFTL
                     REAQLHIRPYTWPYPEISTAAMISPSGPRLAGQLGTSLLSLSMSVPGGYAAIEDAWDI
                     VAEQAKKSGHDEPGRRGWRVLGIMHLADTRDQALDDCTYGLEDFVNYFGAAGFVPLAE
                     TVEGARSSREFVEEYAAKGSGCIGTPDDAIAYIQDLLDRSGGFGTFLMLGHDWASPEA
                     TYHSYELFARKVIPHFNGQLDGPRASHEWAKGKRGQLLGRAGEAIVKAIGEHTDEMKL
                     ESERA"
     gene            complement(9506..10471)
                     /locus_tag="VF874_06345"
     CDS             complement(9506..10471)
                     /locus_tag="VF874_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175719.1"
                     /translation="MVTSTERLVETNGVRLRVVEAGERGAPVVVLAHGFPELAYSWRH
                     QIPALADAGYHVLAPDQRGYGGSSKPDAIEDYNIVALCGDIAGLLDDAGADRAVIVGH
                     DWGAMVAWTLPLLHPDRVAGVVGMSVPPVPRPRKPPIQIWREAFGENFFYILYFQDPG
                     VADADLSRDTRETMLRVFGGPRRPDDHGAAARMLAPGPEGWVERLPEPVALADWISQD
                     EFDHYVTEFTRTGFTGGLNWYRNFDRNWEWTTQTRAATITVPSLFITGSRDVVRTFIR
                     SDRATEVVTGEYEELVIDGAGHWVQQERPDEVNEALLKFLSGLEM"
     gene            10691..12994
                     /gene="ftsH"
                     /locus_tag="VF874_06350"
     CDS             10691..12994
                     /gene="ftsH"
                     /locus_tag="VF874_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9175720.1"
                     /translation="MNRKNVIRTLTVIAVVLLLGWSFFYFSDDTRGFKPVDTSVAVAQ
                     INSDNVKNAQIDDREQQLRLTLKNGNDQTGGSDKIITKYPTGYAATLFDQLTTKNAPV
                     NTVVNQGNMLGSLFIYLLPLLLLVGLFVMFSRMQGGSRMGFGFGKSRAKQLSKDMPKT
                     TFDDVAGVDEAVEELYEIKDFLANPTRYQALGAKIPKGVLLYGPPGTGKTLLARAVAG
                     EAAVPFFTISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFVDEIDAVGRQRGAGLG
                     GGHDEREQTLNQLLVEMDGFGDRTGVILIAATNRPDILDPALLRPGRFDRQIPVTNPD
                     LNGRKAILRVHSAGKPMAPNADLDGLAKRTVGMSGADLANVVNEAALLTARENGTVIT
                     GAALEEAVDRVIGGPRRKSRVISELEKKITAYHEGGHTLAAWAMPDIEPIYKVTILAR
                     GRTGGHAVSVPEDDKGMMTRSEMIARLVFAMGGRAAEELVFREPTTGAVSDIDQATKI
                     ARAMVTEYGMSSKLGAVKYGTEHGDPFLGRTMGTQSDYSHEVARDIDDEVRKLIEAAH
                     TEAWEILTEYRDVLDTLASELLEKETLHRAELEAIFGGVQKRPRLTVFDDFGGRIPSD
                     KPPIKTPGELAMERGEPWPQPVPEPAFKQAIAQASEEAEAARDASGNGNGSNGHPAQP
                     QPVPAATQPDYGAPAGWRAPGWPPQPPQAHWPPQPPQAQWYPPPQTYGPYQQNPQGQS
                     QSEGAPDRQERDERGQEPGRAGPSTQG"
     gene            13014..13616
                     /gene="folE"
                     /locus_tag="VF874_06355"
     CDS             13014..13616
                     /gene="folE"
                     /locus_tag="VF874_06355"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX9175721.1"
                     /translation="MAQPNSRVSRIGVFDRERAEAAVRELLLAIGEDPDRHGLRDTPA
                     RVARAYHEMFAGLYIDPDSVLNTTFDEQHDELILVKEIPMYSTCEHHLVSFHGVAHVG
                     YIPGEDGRVTGLSKIARVVDLYAKRPQLQERLTGQIADALMRKLDPRGVIVVVEAEHL
                     CMAMRGVRKPGAVTTTSAVRGQFKTDNASRAEALDLILRK"
     gene            13613..14443
                     /gene="folP"
                     /locus_tag="VF874_06360"
     CDS             13613..14443
                     /gene="folP"
                     /locus_tag="VF874_06360"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9175722.1"
                     /translation="MSTTRVQVMGVVNVTDDSFSDGGLYLDRDRAVEHGQALAADGAA
                     IVDVGGESTRPGATRVDPRVETDRVVPVVKALAAQGITVSIDTMRAEVAAAALQHGAR
                     MVNDVSGGRADPKMAPLVADADVPWILMHWRSVSAARPHDVPDYGDVVADVKAELLAS
                     VDAATSAGVDATRLIIDPGLGFAKTAQHNWALLRALPELVATGIPVLVGASRKRFLGS
                     LLADEHGDRPPAGRETATAVVSALAALQGVWGVRVHDVRATVDALKVVEAWKAGLRGD
                     "
     gene            14436..14816
                     /gene="folB"
                     /locus_tag="VF874_06365"
     CDS             14436..14816
                     /gene="folB"
                     /locus_tag="VF874_06365"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX9175723.1"
                     /translation="MTDRILLHGLTVRGHHGVFEHERRDGQEFVVDITLWIDLDRAGA
                     SDDLADTIDYGELAKRAADIVGGPPRNLIEAVAADIADDVMRDERVHAVEVTIHKPQA
                     PIPLAFTDVAVVARRSRRSGRVTG"
     gene            14813..15334
                     /gene="folK"
                     /locus_tag="VF874_06370"
     CDS             14813..15334
                     /gene="folK"
                     /locus_tag="VF874_06370"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX9175724.1"
                     /translation="MTRAVLSIGSNLGDRLARLQSVVDGLGDSVVAVSPVYETVPWGG
                     VEQGPFLNAVLIADDPDLDGLGWLRLGQKLEKAADRVRGERWGPRTLDVDLVSCSADG
                     RELVVREPDLTLPHPLAHLRAFVLVPWLAIDPEAELMIAEVSTPVTQLLDDIEPVDKA
                     GVMPTDLTLRSTV"
     gene            15336..15812
                     /locus_tag="VF874_06375"
     CDS             15336..15812
                     /locus_tag="VF874_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HEX9175725.1"
                     /translation="MGPTRKRDLTAAVVAVAVVGYFVTAAVYPGWFPPITVWTGLSLL
                     GVAAVEAAWGRHVRSKINDGMIGDGGGRLHPIAVARTVTIAKASAWVGALMLGWWFGV
                     LAYLLTNRSLSVAVSDTPGAAVAAASALALIIAALWLQHCCEKPEEPPDSPGATAE"
     gene            15867..17012
                     /locus_tag="VF874_06380"
     CDS             15867..17012
                     /locus_tag="VF874_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6779 domain-containing protein"
                     /protein_id="HEX9175726.1"
                     /translation="MTDLSRGGRSRRSDRRPGWLLLSVLLVLAIAASSALVFTERVEL
                     LKLAVILSLWAAATAAFVSVIYRRQSDLDRAKARDLKLVYDLQLDREISARREYELEV
                     ESQLRRELASELRAQSAEEVAELRAELAALRNNLEILFDADLSHRPAIEPERTTVRAY
                     SDWERDTEITDPGRNIPRYVESTPRQHPDDDTENAIIDVPEEPPVPEEEVRVASEPHQ
                     PEQVWTPPQPEPAAHAEQPAERGSRHGLRAEREWQPVPSAGQWLPPGAPGSNWVSTNG
                     TAPTPGSAPPPPVPAEPGLGRHSNPVEPVGAPAAPPPQHSTESVGYAPAPPPPQQPPA
                     RHDDAGDSAPGVGQHGEGQGQSVAELLARLQAGTGPTAGGRRRRRDG"
     gene            17093..18058
                     /locus_tag="VF874_06385"
     CDS             17093..18058
                     /locus_tag="VF874_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX9175727.1"
                     /translation="MEQSPTHAWGPPDGLRPARLLVGIISAGRVGTALGSALERAGHV
                     VVACSGVSHASRERASRRLPDSAILPAHEVAARGELLLLAVPDAELASVVSGLAATRA
                     VRPGTIVVHTSGANGIGVLAPLTEQGCLPLAIHPAMTFTGSDEDIARLPDTCFGITAA
                     DDVGYAIGQSLVLEVGGEPFRVREDARTLYHAALAHASNHVVTVVADALEALRTALWG
                     QELLGQQLVDDAPGGIAERVIGPLTRAAVENTLQRGQAALTGPVARGDADAVAAHLRA
                     LAEVDPRLAEAYRTGSLRTAQRDHASPEIFAALDNSPDRTGCGDN"
     gene            18048..18983
                     /gene="panC"
                     /locus_tag="VF874_06390"
     CDS             18048..18983
                     /gene="panC"
                     /locus_tag="VF874_06390"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX9175728.1"
                     /translation="MTINLNKKFHPGQLNVYETPKAVAEVTRALRQTGRRVMLVPTMG
                     ALHAGHLALVRAARRVPGSVVVVSIFVNPLQFGAGGDLDSYPRMMDADLDLLRREGVE
                     VVFAPSVEAMYPDGPRTSVQPGPLGAELEGASRPTHFGGMLTVVLKLLQTVAPDRAFF
                     GEKDYQQLALIRQMVADFNLDVRVVGVPIVREADGLALSSRNRYLTPTERELAGALSA
                     ALLAGMYAASGGADAALDAARAVLAEVPALEVDYLEIRGPALEPAPAAGLGRLLVAAR
                     LGDTRLIDNIAIDIGGPDGQSSDGSDDHHELPWRN"
     gene            18983..19417
                     /gene="panD"
                     /locus_tag="VF874_06395"
     CDS             18983..19417
                     /gene="panD"
                     /locus_tag="VF874_06395"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX9175729.1"
                     /translation="MLRTMLKSKIHRATVTEADLRYVGSVTIDADLMDAADLLEGEQV
                     TIVDVTNGARLVTYAITGKRGSGVIGINGAAAHLVHPGDIVILIAYGTMTDAEAREYR
                     PRIVFVDADNKQVDLGADPAFVPDQYSGLMSLSTGSNLVSPR"
     gene            19420..20235
                     /locus_tag="VF874_06400"
     CDS             19420..20235
                     /locus_tag="VF874_06400"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX9175730.1"
                     /translation="MLLAIDVRNTHTVVGLITGSGVHSKVVQHWRIRTESQATSDEMA
                     LTIDGLIGDDSGQLTGAVALSTVPSVLHELREMLDQYWPRVPHVLIEPGVRTGIPLLV
                     DNPKEVGADRIVNCLAAHQKYGTAAIVVDFGSSICVDVVSAKGEFLGGAIAPGVEVSS
                     DAAAARSAALRRVELTRPRSVIGKNTVECMQAGAVFGFAGLVDGLVRRIRDDVDGFAG
                     DDVTVVATGHIAPLLLPAMHTVQHYDRHLTLDGLRLVFERNRDSQRGKLRQAR"
     gene            20526..21398
                     /locus_tag="VF874_06405"
     CDS             20526..21398
                     /locus_tag="VF874_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEX9175731.1"
                     /translation="MNGLKSQAQSSHRRLSRRPLPTWSVATLVAATVFATVVTGCGSK
                     SSTPSETTGASGTTATSATATTPGEPLPEASKILQASAKTTQTLHSVHIALTTTNLPD
                     LPMETVNADVTNLPQGSARAVGDAKVRTQPNAPVNEFSFVVTDKTLYTKGADGKYTSM
                     GPAQKIYDPGIVLSKDKGLAHLIEQVQNPKVDGRETINGIVTIKVSGTIDAAVIDPIV
                     PQLGKDGGTLPITLWIADVPASATPTTTLPSDAPSPGTGPNLVRFMVNKDPGNVEVTL
                     SNWAKPVDIPNPTG"
     gene            21963..23444
                     /locus_tag="VF874_06410"
     CDS             21963..23444
                     /locus_tag="VF874_06410"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX9175732.1"
                     /translation="MTNDPPQVQERDLPQARERDSRRPPRRAGGPANVWVYKGKAYDL
                     TDWISKHPGGAFFIGRTRGRDITSIIGSYHRNPAAIERVLERYALGRDATPEDIHPKN
                     NAPPFLFKDDFNSWRDTPKFRFDDKNDLLHRVKTRLKDRQLATRIRKMDTVFNIVVGL
                     LAVAYLAVQGLRLAEPAWMPLPLFVIAMVLLRSSLVGFGHYAIHRAQKGVTRFLSSSF
                     DLNYVALALVVADGHTLLHHPHTQSEVDIKKNVFTMMMRLPRLYRIPVHTVHKFGHLV
                     SGMAIRIVDVVKITRKVGVEKLYGSWRNALAHLIGSSGVRLLLIGELVAFILAGDFWA
                     WALQFVATLWVSTLLIVASHDFEEDADEVQSDTEDWGIHQLEESYDLKVIGNRYVDCF
                     LSAGLSSHRVHHVLPYQRSGFANIATEDVLREEAAKFGVEWLPAKSFVTDRLPKLCRT
                     YLLSRSRQAAEQNWGFVREHFSPAAFKTSANYVAGGFAGLGSV"
     gene            23466..24641
                     /locus_tag="VF874_06415"
     CDS             23466..24641
                     /locus_tag="VF874_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX9175733.1"
                     /translation="MFDEAETCGPAGEAVGLLAELPPSPPTTVAVIESIATGAPGRMV
                     DQCGAADRVAALFAEDPQQRARIARVYRKTRIASRQMAVDPLDAEFDGVRREPAMVRN
                     RMNLFYQHAVPLAVDVAGRALAGGGHVASEIGLLVFVTSTGFIAPGVDVAVVKQLGLS
                     PSVSRVVVNFMGCAAAMNGIRTAADHVRAHPDTKALVVCIELCSVNAVFADDINDVII
                     SSLFGDGCAAVVVGASPVKHPLAPGKVVIRRSFSRLLDDAEDGIVLGVNHNGITCELS
                     ENLPGYIYDGVDPVVTEVLADNGLRKSDVDLWAIHPGGPKIIEESVRSLGIPADAAAA
                     SWEVLARYGNMLSVSLIFVLEVMVANARSTKAISTGVAFSFAPGVAVEGMMFDIIRH"
     gene            24670..26463
                     /locus_tag="VF874_06420"
     CDS             24670..26463
                     /locus_tag="VF874_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007319213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9175734.1"
                     /translation="MQLIKFLISISPGRIAAVFVAGLVGGVANTYLLTLIGSVVSPAA
                     HHGATLQRFAITGLIALVSAVLSQTLLIRLTQDAIYHLRAKFCSEVVALPLEHLERLG
                     MHRLLGTLTEDVRSLSQAVTAIPNVCVAAVTIVGCLAFLALVSGPIFAVTVAGLAVGT
                     LGVEALSRRVRHLYTEARDNEDALLRSFQSVTSGIKELKLHRGRRRDFMDRHLLGTAG
                     VLRDQNVAAGSRISLANSYGRLLQLVTMAAILFVLARTLDVPRSVMVTYILITMYMSV
                     PTQNFMQRIPDLRRGNVALAKIRAMDLSMQTKHDEQHLPHSDRPTATTARLELTNLSY
                     TYKAEAAPPFPKGPTAVGNPPRGGPHSGRHGPGGKMAGRPPGPLAGVDAGESGFRLGP
                     LDLLFEPGQISYVVGGNGSGKSTLAKLLTGLYSPETGFVTLNGEKIGHDNIDWFRQNC
                     SAVFTDFHLFEEYLGFVRPGIDDEVRHYLAELQLAHKVSVQDGRLSTVALSQGQRKRL
                     ALLTALLEDRPIYVFDEWAADQEPRFRDVFYTEILTGLARRGKTVIVITHDDRYFHCA
                     DQIVKLDYGHIEDITSPAPVRPATPRRPAAS"
     gene            26825..27778
                     /locus_tag="VF874_06425"
     CDS             26825..27778
                     /locus_tag="VF874_06425"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010924390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEX9175735.1"
                     /translation="MSDNRRWDALELREGDIVISAPPKCGNTWTQRLVSLLVFDGPDL
                     PGPLSKVSPWLDQTTMPVEEVVAALKAQGHRRFIKTHTPLDGLVLDDRVTYIGLGRDP
                     RDAVMSMIMDWDRMRALRHATMPPNERVGPAGPRPRRVSPLEAFQEWMDWPAMPTVGG
                     RPSTLRPGAAEPRSGIWVSLANFLHHFDSFWSLRHLPNVALFHYADFQLDLAGEVVRL
                     GQVLDLDVGRDRAEELAKHGTLDAMRARAAEFAPDADRGIRRGDSPFFRAGGRGTWRD
                     IATEDVLRRYNQRAGRLASPDLLAWAHEGRRGHEPDNATSP"
     gene            complement(27775..29016)
                     /locus_tag="VF874_06430"
     CDS             complement(27775..29016)
                     /locus_tag="VF874_06430"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX9175736.1"
                     /translation="MLDRTTVAALADEPVDWRFKGLPPSWTGRTPRQICADRPALFDD
                     GPTGPVCVLDADALTHNLTTMANWCRDAGVEIAPHGKTHMAPQLFARQLDAGACGITA
                     ATVSQVRVCRGFGVRDVILANQLVDPGGLRWLAGELDRDPEFGFVCWVDSVPGVQTMA
                     AELATCATRPVDVCVEVGMAGGRTGCRSAAAVDDVARAVVACPYLRLVGVAGYEAALG
                     HDVSVEALSSVTEFLGDIRSAVQRLAFESDEVLVTAGGSTYFDLVADVLAAGWPSGLQ
                     VRTVIRSGAYLTNDHGLYQRTSPLTRTGRHGLWPAMSVWAQVTSIPEPELAVLTIGRR
                     DVSFDQDLPTPLALPDSRISRLNDQHAYLHVGPADRSRIDVGTWLEFGISHPCTVFDK
                     WQLIPVVDSDGRVVDLIRTFF"
     gene            29040..30548
                     /gene="lysS"
                     /locus_tag="VF874_06435"
     CDS             29040..30548
                     /gene="lysS"
                     /locus_tag="VF874_06435"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX9175737.1"
                     /translation="MSSADPCEPDVPEQFRIRQGKRERLLDEGNDPYPVNVPVTHTLK
                     QIRENYPDLPADATTGEIVGVAGRVVFARNSGKLCFATLQQGDGTQLQVMISLASVGQ
                     EVLDRWKSEVDLGDIVFVHGEVISSRRGELSVLADAWRMAAKALRPLPVAHREMTEES
                     RVRQRYVDLIMRPEARAVARQRVAVVRALRNALHRRDFLEVESPMLQTLAGGAAARPF
                     VTHSNAMDSDLFLRIAPELFLKRCVVGGFDRVFELSRVFRNEGADSTHSPEFSMLETY
                     QAYGTYDDSAVMIREVIQEVADDVFGTRQLTLADGTRYDIDGQWVSIEMYPSLSEALG
                     EPITPDTSVQHLVSIATRLGIEIPTDRPFGHGKLIEELWEHSVGRNLYAPTFVRDYPV
                     ETTPLTRQHRSIPGVTEKWDLYVRGIELATGYSELIDPVVQRERFEAQARAAAAGDDE
                     AMVLDEDFLTAMEYAMPPTTGTGMGIDRLMMALTGLGIRETVLFPIVRRQAN"
     gene            30673..31011
                     /locus_tag="VF874_06440"
     CDS             30673..31011
                     /locus_tag="VF874_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEX9175738.1"
                     /translation="MAKKVTVTLVDDFDGEAAADETVEFGLDGVTYEIDLSSKNAKKL
                     RDDLKVWVEKGRRVGGRRRGRGSSGRGRASIDREQSAAIREWARRNGHNVSTRGRIPA
                     DVIDAFHAAT"
     gene            31289..>33645
                     /gene="clpC1"
                     /locus_tag="VF874_06445"
     CDS             31289..>33645
                     /gene="clpC1"
                     /locus_tag="VF874_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpC"
                     /protein_id="HEX9175739.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKSLESLGISLEGVRSQVEEIIGQGQQAPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGAELTRVRQQVIQLLSGYQGKETAEAGTGGRGG
                     ESGNPSTSLVLDQFGRNLTAAAMEGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEP
                     GVGKTAVVEGLAQAIVHGEVPETLKDKQLYTLDLGSLVAGSRYRGDFEERLKKVLKEI
                     NTRGDIILFIDELHTLVGAGAAEGAIDAASILKPKLARGELQTIGATTLDEYRKYIEK
                     DAALERRFQPVQVGEPTVPHTIEILKGLRDRYEAHHRVSITDSAIVAAATLADRYIND
                     RFLPDKAIDLIDEAGARMRIRRMTAPPDLREFDEKIAEARREKESAIDAQDFEKAASL
                     RDREKTLVAQRAEREKQWRSGDLDVVAEVDDEQIAEVLGNWTGIPVFKLTEEETTRLL
                     RMEEEIHQRIIGQEDAVQAVSKAIRRTRAGLKDPKRPSGSFIFAGPSGVGKTELSKAL
                     ANFLFGDDDALIQIDMGEFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKPFSVVLF
                     DEIEKAHQEIYNSLLQVLEDGRLTDGQGRTVDFKNTVLIFTSNLGTADISKPVGLGFT
                     QGGGENNYERMKQKVNDELKKHFRPEFLNRIDDVIVFHQLTTEQIVQMVDLMIARVEK
                     QLKSKDMAMQLTDKAKALLAKRGFDPVLGARPLRRTIQREIEDALS"
BASE COUNT         5763 a        11014 c        11227 g         5641 t
ORIGIN      
        1 caccgatgac tgcgccgagc acggcgacga cgatgacgag agcgatgttc atgtggccgg
       61 tgccggcgaa gatggccgcc gtgatcagga tggcctcgcc aggcaccggc acaccgaagt
      121 cctcgagcag cacgaaaccg ccgaccgcca catacccgta gtgctccagg actggctgca
      181 catcgccgaa caccccaggc agctgcccat ggctcatggt gtgctccgtc cggatttcgg
      241 gaacccgtcg catggtagcg acggtgccga cgcgccggat atcggctcgg catgagacac
      301 tgagatcgga ggggagtatt ccttcgtcgc ggtgtcgtca tcacgtcgcc catgattgga
      361 cggccggccc gcgggccgtg atggcggcgg aagagacctc cggcgttttc gtgacgaccg
      421 gaggattcgg atgaacgttt cgcaactcga gtggatcatc accctcgggg cgacgatcgc
      481 tgtgttgctg ttcgacgtga tgatcatcgg gcgtcgtccc cacaagccga gcatgcgtga
      541 atgcgcgatc tacctgacga tctacgtcgg gctcgcggtg gcgttcggag tattcgtctg
      601 gttcttccac ggcagccaat tcggcctcga gttcttcgcg ggatggctta ccgaatacag
      661 cctgtccgtc gacaacctgt tcttgttctt gctgatcatg gcgagcttca acgtgcccga
      721 aaagtaccag cagcaaacgc tgttagtggg catcgtgctg gccttggtgt tccgcggcat
      781 cttcatcgcg ctcggtgccg tcgcgatcaa cgagttctca tgggtcttct accttttcgg
      841 cgcgttcctg atctacaccg ggtttcacct ggcacgcgac aaagaccacg acgacgacgc
      901 ggacaaccgc gtggtgcggt tcgccaagac gtacctgaac accaccgatc ggtgggacgg
      961 cctcaagctg tacgtgaaag agggctcgaa gcgcctgatg acgccgatgt tcctcgtcat
     1021 cctggcgctg ggcaccaccg acatcatgtt cgcgctggac tccatccctg cgatctacgg
     1081 gttgaccagc gagccgtacc tcgtgttcac cgcaaatgtg ttcgcgctga tgggtttacg
     1141 gcagctttac ttcttacttg gtgacctgtt gaagcggctc gtgtacctct cgcagggact
     1201 cgcggtgatt ctctgcttca tcggcgtcaa gttgacgctg cacgctctgc acgaaaacga
     1261 agtgccgttt atcaatggcg gcaagcacgt tgacgtgccg gagatcccga cgctgttcag
     1321 cctcggcgtg attgtcgcga cgctgatcgt caccaccgtc gccagcctgg tcaagacccg
     1381 ggtgattgac gcgaacagcg acgacgacga cgcggtcgcc gaagaagggc cggcagagtc
     1441 tgagcgaacg gagtgacgtt caggcttagt gcagccgtag cgctacttcc agtgaccgcc
     1501 gtgctggctg cacaccagtc cggtgctgtt gcacttgttg caaccaggct tgccgccgat
     1561 gccactggcc cggccgccgt tgcatccctg gcagtcgtga agtggccgac gacatttgcg
     1621 gtattccatg gcgagtcctt tcgacgctag tccggtccgt tccagaatct tcactcgcgg
     1681 cggctgcgct cggtatacgc cgttcgacgg acaaagccac cccggctacc gggtggccgc
     1741 tcagccgagg gttttgtaga acagcctggc gtacctcgcg gtgcgctcgg aaccctcgat
     1801 gccgggaccc atcttgttca tcacgtgcgc gaaagtggcg cgatggtcgg ggttcatcag
     1861 ctcccacgag ccaccccagc cgccccagaa gcagatcttg ccgtcgggta cgtacgggaa
     1921 cgtgtcgcgc tgaggcaagc cgtatcccac cccccagcgc accggaggtt gggcgagcac
     1981 gagatcaggc ccgtcgcact gcacttcgaa aatcttctcg acggtctcgc gccgcagtag
     2041 ctgaacgcca ttgacgttgc cgcccaacga aatagcggac agaatcccag gctggtgatg
     2101 ttcttcgtcg acgaccacac gttgacgatg gtgtctttgc cccactcgcg agtttcgccg
     2161 cgtcggcgtg gccaccccag acgtcgacga caagctcgcc gtcgatgtcg accgcgatcg
     2221 ccgcgcctac ttcctcgccg ctggtgagct cctcggccat tgccgcccgc aagctgttga
     2281 actgaggatc gcactgacca tggatgcttc gctcacccga ccatcatgcc cgcggcgatt
     2341 accagttgtg gtagccgcct tcggggccga gcatgcgctc cagccgggtg cggttgatct
     2401 tggccagctc gttgcggacc accttgcgtt cggtgtcgat ctcgtggtgc agccgatcgg
     2461 aaatcgccgc cagcacaccc tcggcgtcgg tgccgtcgac gaacatcacg aagctgaggc
     2521 cgaactgctc ggtgtagcgc tgcgcggccg tgtcgagttg agccatcacc tcgggccgcg
     2581 ggtcccacac tgagcactgc tcggcctgcg atttcgtgct gcgcggccgt cgcccgatgt
     2641 gcggataggc ctgcaggatc gtgtccaccg acgactcgga caacgcgaac agcaacgcgt
     2701 cggcgcggcg caacaactcg tcgtggctgg cgtagggccg cccgccggcg aggtcgcgcg
     2761 ccagcaccac gctgttgcag cattcataca gcgcatgcat tgccttttgc ggcgacagct
     2821 cgttgaacgc gtcgaggccg atgccttgat gcatcaacac ttttggtccc cttcgatggc
     2881 ggttgttatc gccaatcatt gggggccgtc cgaccatgtt tccagtcgaa acggcaaaat
     2941 tgacctgcgc gtaacaggcc gtaactccgg cgtttcagcc gaacaggccg aaagtcagtg
     3001 accgccggct tggaaacgct ccagcgaccg cagcacctcg gcctcggcgt cctcgcggcc
     3061 cacccagtca gcggcctcca cgtacttgcc cggctccagg tccttgtagt gcacgaagaa
     3121 gtgcttgatc gcgtccagtt cgaaggacgg aacatcgccg atgtctttga cgtggtccca
     3181 gcggtagtcg ccggcgggca cgcagagcac cttgtcgtcg ccaccggcct cgtcggtcat
     3241 ccggaacatc ccgaccggcc gggcttcgac gatcacgccg gggaacaccg actcgggcag
     3301 cagaaccagc gcgtccagcg ggtcgccgtc ctcgccgagc gtgtcctcga tgaacccgta
     3361 gtcggtgggg taggccatcg ccgtgtacag gtagcggtcc agccgcaacc ggcccgtctc
     3421 gtggtccacc tcgtacttgt tgcgctggcc cttcggaatc tcgatggtca cgtcgaattg
     3481 cacgcgccgg gctccttcac tgtcggacgt cggtgttaac catagtggag ccgctaacct
     3541 tcactacatg catccggcgc ggtggcggcg ctccacccat ttcctgatcg gcgcggccgt
     3601 gctggtcgtg gtcgtggtgg cggccgctgc gctgctgggc aacggcggct cgagccgcag
     3661 cgccaaggcc gcgccggcga cgccccctgc ggcggccgcc aagctctcgg tggttccggt
     3721 ggtcgacgcg gcgccgcttc ccacgccagc cgggctcgcc gcggcgttga agcagccgct
     3781 ggccaaccct gacctgggcg cgctgaccgg ccgcgtcacc gacgcgctga ccggcacgga
     3841 gttgtggcaa caggggggca ccacgccgat ggtgcccgcg tccaccaaca aggtgctgac
     3901 cgccggcgcc gcgctgctca cgctcaaccg tgacacccgc cagaccacca cggtggtggc
     3961 ccccgaccag agccgccagc ccggggtcgt ggtgctcgtg gggggcggag acccgacgct
     4021 gtcatccgcg ccgccgggcg aggagacgct gtaccgcaac gcggcccgca tctccgacct
     4081 ggccgcccag gtgcgcggca gcggaatcaa ggtcaccgcg attcaggtcg acgccagcgc
     4141 gttcagcgga ccgtcgctgg ccccgggctg ggacccggtt gacatcctgg gcggcgacgt
     4201 cgcgccgatc gaggccgtga tggtcgacgg cggccgaaca caaccgctgt cgccggagtc
     4261 cgtgcggtcg ttcaccccgg cgctcgatgc gggccaggct ctggccgccg cgctgaacat
     4321 tgatccgcag gcggtcacga tggccgcagt cccgatgcgc gacggccgcc ggattgcgtc
     4381 cgtgcagtct gcgccgctga tggaccggct caacgagatg atgaaccatt ccgacaacgt
     4441 gatggccgaa tgcatcgcgc acgaggtggc cgtcgcggtg ggccggccgg cgagctttgg
     4501 tgacgcggtg caggcggtca cgagccggct ggccgccgcc ggcgtggaca tgacgggctc
     4561 ccggctgatg gacggcagcg ggctctcggt ggacgaccgg ctcaccgcgc gcacgctcga
     4621 cggggtgatc ggcgccgccg cgggcgtgtc agcgccggcg atgaggcccc tgctcgacgt
     4681 gctgccgatt gccgggggca gcggtacgct gtccaaccgc tatatcgacg accaaactga
     4741 cgaggcggcc ggctggctgc gggccaagac cggctcgctg acgggaacga acacgctggc
     4801 cggcgtcgtc accgacgtga gcggccgggt gctgacgttc acgctgatgt ccaacaacgc
     4861 ggggcccact ggacgcaccg cgatcgacgc gctggcggcg acgctgcgca gttgcggatg
     4921 tggctcgtga cgcctacaca ggacagccgc aagctgacgg tcggccgggt ggtcgactgg
     4981 gacttcgccg ccaccaccgg cgcccggctt gcccgcccgg ggccgccgtc cagcgactac
     5041 acccagcgcc agatcgtcga cgaactcgcg gagtcgtcgc ggcgcgcgga ggtaccggtg
     5101 cgggaagtca ccggcctggt gcccggcgga gaggtgcccg acgcgctgat cgtcgaccgg
     5161 ccgcactggg ttcggctcgc cgccagctcg atgcgggtga tgaccggcgg cgacgacgaa
     5221 ccgcgcggct tcctgcgggg gcgcatcacc ggcgcgcaga ccggtgcggt gctggcgttc
     5281 gtggcgtcgg gaattctggg ccagtacgac ccgttcgcgg ccagcggcgg caagctactc
     5341 ctgatctatc cgaacatcat cggcgtcgaa cgccaattgc aggttgtgcc aagagatttc
     5401 aggctctggg tgtgtctgca cgaagtgacg caccgcgtgc agttcaccgc caacccgtgg
     5461 ttggccgact acatgtcgtc ggcgctcggc gtcatcaccg aagacgccgc tgacgacttc
     5521 ggcaacgtgg tgggcagact cgctgactac gtccgcgatc gtcgcgacgg cgcaccggcc
     5581 gaaccgaact cgaacgggat catcggcttg ctgcgcgcga tgcagtcaga gccacagcgc
     5641 caggcgctcg accagctgct ggtgctcggc acgctgctcg aaggccacgc tgaccacgtg
     5701 atggacgcgg tcggaccggc ggtagtgccg acggtgacga cgatccgcga gcgcttcgac
     5761 gaacgacgtc gccgccggca gccgccgctg cagcggttga tccgcgcgct gctgggcttc
     5821 gacgccaaga tcagccagta cacccgcggc aaggcgttcg tcgacgcggt cgtgtcgcgg
     5881 gtcggtatgg aacggttcaa cgccgtctgg tcgggtccgg acacgttgcc gctgcccgat
     5941 gaaatcgaca aacccgagcg atggatcgca cgggtgctgt agccgcactg ggcgaagcgg
     6001 tgtcggcctt cacgctgcgt tactccatca gcgaaaagcc ttggtgtgtg gcgttttcgg
     6061 gtggtcccga ctcgttggcg ctgaccgcgg tgtgcgtggg catgcggccc accacggcgc
     6121 tgatcgtcga tcacgggctg caggacgggt ccgctgcggt cgccgataca gccaggcgcc
     6181 aggcgcttga tctcgggtgc gtggaggcgc aggtcctgcg ggttgacgtg ggccggcttg
     6241 gcgggccgga agccgccgct cgaaccgccc ggtacgcggc gctgcaggag gcgagtgccg
     6301 gcgcgccggt gctgctcggc cacaccctcg acgaccaggc agagaccgtt ctgctgggcc
     6361 tcggccgcgg ctcgggcgcc cggtcgctcg cgggcatgcg gccgtatcag ccgccgtggt
     6421 gccgccctct gctgggcgtg cgacgggcgg tgacccacgc cgcgtgcgac gaactcgggc
     6481 tcagcgcgtg gcaggacccc cacaattccg atccgcgcta cacccgctcg cgtctgcgcc
     6541 gcgacgtggt tccgctgctc gaggacgtgc ttggcggcgg ggtcgccgag gcgctggccc
     6601 gtaccgccgc agcgctgagg gaggactgcg atgcgctcga cgacctcgcg cgcaccgaac
     6661 tcgacgcggt ggtcaccgac caggggctgg acgcgcgtca gctgaatgac ctaccggatg
     6721 cgttgcgccg cagggtgatc cgcggatggc tgctggcggg cggggcgacc gacctcaccg
     6781 acaaccagat tcgccgggtg gacgaactcg tgatgaactg gcacggccaa ggcggcgtgg
     6841 cggtcgggtc cgcgctgcgc agtcaacggc tggtggcggc ccgccgcaac ggcaggttgc
     6901 ggctgcaccg cgaaccggtc tgactactgc ggtggtctgc gcacttgcgg catggcaccc
     6961 tgatggcgtg gcagcgcagg cggcagccga catgtattcg ggcgacatca agtcggtgtt
     7021 actcaccgag agtcagattc agaaccgcac ggccgagctg ggcgccgcca tcggcgagcg
     7081 gtatcgcggc ttgatcgaca gcagcggcga cgacctgctg ctggtcaccg tgctcaaggg
     7141 tgctgtgatg ttcgtctccg acctggctcg cgcgatcccg ctgccgacgc agctggagtt
     7201 catggccgtc agctcctacg gttcggcgac gtcgtcgtcg ggcgtggtgc gcattctcaa
     7261 ggacctcgac cgcgacattc acgaccggga cgtgctgatc gtcgaggaca tcgtcgactc
     7321 cgggctcacg ctgtcgtggc tgctgcgcaa cctcgccgcc cggcacccgc ggtcgctgcg
     7381 ggtgtgcacg ctgctgcgca agcctgatgc ggtgcgctcg gacgtgggca tcgacttcgt
     7441 cggcttcgac atcccgaacg agttcgtggt cggctacggg ctggactacg ccgagcgcta
     7501 ccgggacctg ccttacatcg gcacgctgga cccgcggatc taccaggagt agctctcgcg
     7561 tctgttcggc gggctaggtg agctcccaga tcaccgtcac cgagaagctg accgtctgct
     7621 ggccgggctc gagcggaacc gcttccgtcg cagcccgtgg catcggagtg ggtggtgtgg
     7681 ccccgcttgc ctcggagatc gagatgacct tgcccagccg cagccccgac agctcggcat
     7741 actgctcggc gcgagccttg gcgtcatcga acgcccgtgc ccgcgcgtcc ctcaccaact
     7801 gcgaatcgtc gtcgatggag tagttgaccg agtcgatcct ggtggcgtcg cctccggtgc
     7861 tgacgatcag cgcgagtgtc cgcgacgccg agccgaggtc gcggatcttc acggcgagag
     7921 agttgctgga acggtagccg atgatcgccg acgagtcgga gccgtactgc ggctgcaggc
     7981 tgaccgacgt ggtgctgatg tccttgcggt cgactttcgc atcgttcacc agcgcgtcga
     8041 tcaccgcttg ggtacgaccg ctggtctgat tcatcgcggt ggtgacgtcc ggcgcggtgg
     8101 cctcgatcga aacgttcgct gtcagcgtgt ccggggtgcc ctgcacctgc ccggcgccga
     8161 tcacggtgac ctgcctgttg ttcgggcccg cttgcggcga cgagtccgat tgctcgtcac
     8221 acgccgacaa ccccgcgatc gcgaaggcgg aggtgaccag cacaactcca gtgcgcagca
     8281 ccgtctttac agcgatcgga ataccccgca catcaggccc tttcactttc caacttcatc
     8341 tcatcggtgt gttcaccgat cgccttgacg atcgcctcgc ccgcgcgccc gagaagctgg
     8401 ccgcgcttgc ccttcgccca ctcgtgcgac gcccgcggtc cgtccagttg gccgttgaaa
     8461 tgcggaatca ccttgcgcgc gaacaactcg tacgaatggt aggtcgcctc cggtgacgcc
     8521 cagtcgtgcc ccagcatcag gaacgtccca aagccgccgg aacggtcgag cagatcctgg
     8581 atgtaggcga tcgcgtcgtc gggtgtgccg atgcagccac tgcccttggc cgcatactct
     8641 tcgacgaact cccgcgacga ccgcgcccct tcgactgttt cggcgagcgg cacgaatcct
     8701 gctgcaccga agtagtttac gaagtcctcg agcccgtacg tgcagtcgtc cagagcctgg
     8761 tcgcgcgtgt cggcgaggtg catgatgccg agcacgcgcc agccgcgccg gccaggttcg
     8821 tcatggcccg acttcttggc ctgctcggca acgatgtccc aggcgtcttc aatcgcggca
     8881 taaccacccg gcacggacat cgacagcgac agcagcgacg tgccgagctg acccgcgagc
     8941 cgcggacccg acggggaaat cattgcagca gtggatattt cagggtacgg ccaggtgtag
     9001 gggcggatgt gcagctgagc ctcgcgcagc gtgaaccagt ccgagtgcct gtcgatgcgt
     9061 tcgccgggcg cggcgcggaa cagtgcgaga atggcctcaa gggactcgtg catcatccgg
     9121 cgctgttcga cggggtcgat gcccatcatg tacgcgtccg acggcagtgc accggggccg
     9181 gtgccgaaca tcgttctgcc gcgggtcaag tgatccagca acacccaacg gtcggccacc
     9241 atgagcgggt ggtggtacgg cagcgacacg acaccggtgc cgagccggat gtgtttggtg
     9301 cgctccgcgg cggcggcgat gaacacctcg gggcacgcga tgagttcgta accgccggaa
     9361 tggtgttcgc cgaaccacgc ctcgtcgtag cccagtcggt ccagcgcgac gacgcgctcc
     9421 agatcgtatt ccagtgcgac cgtgggggat tggccgacgg ggtgaaacgg cgtgatgaag
     9481 acaccgaagc gcaagggacc ttccatcaca tctccaatcc ggacagaaac ttcagcagcg
     9541 cctcgttgac ctcgtccggc cgctcctgct ggacccagtg accagccccg tcgatcacca
     9601 gttcttcgta ctcgcccgtc acgacctccg tggcgcggtc ggaccggata aacgtccgca
     9661 cgacatccct tgagcccgtg atgaacagcg acggcacggt gatggtggca gcacgcgttt
     9721 gagttgtcca ctcccagttg cggtcgaagt tgcggtacca gttcagcccg ccggtgaagc
     9781 cggtccgggt gaattccgta acgtaatggt cgaactcgtc ctggctgatc cagtccgcca
     9841 gcgcaaccgg ctccggcagc cgttccaccc atccttccgg cccgggtgcg agcattctgg
     9901 ccgccgcccc gtggtcgtcg ggccgtcgtg ggccgccgaa aacgcggagc atggtctccc
     9961 tcgtgtcgcg gctcaggtcg gcgtcggcaa caccaggatc ctggaaatag aggatgtaga
    10021 agaagttctc gccgaacgcc tcccgccaga tctggatcgg tggtttccga ggccgaggca
    10081 ccggaggcac actcatgccg accacgcccg caacgcggtc ggggtgcagc agcggcagcg
    10141 tccaggccac catggcgccc caatcgtggc cgacgatcac cgcgcggtcg gctccggcgt
    10201 cgtcgagcag cccggcgatg tcgccgcaca gcgcaacgat gttgtagtcc tcgatggcgt
    10261 ccggcttcga cgaccccccg tagcccctct ggtccggcgc cagcacgtgg tagcccgcat
    10321 cggcaagggc cgggatctgg tgccgccacg agtaagcaag ctcggggaat ccatgcgcca
    10381 gcaccaccac cggggcgccg cgctcaccag cctcgaccac gcgcagccgc acgccgttcg
    10441 tctcaactaa ccgctcggtt gaggtgacca cggtctcacc caagcacagg ggcggctgct
    10501 cgtgaagggg ccgcatgtgc agcgaaaccg gggtttcgca cacaaagtgc gggtgccgac
    10561 gtgcgaaact ccggtttcgc cgaaagggcg aacgatcccg tcgggaacta tcgatcgttg
    10621 gtctaacggt agccttgact atctcctcgc acgcatttgg aaggacctgg ccggccccgg
    10681 ccgacaagtg atgaaccgga aaaacgtaat ccgcacactc acggtgatcg ccgtcgtgtt
    10741 gttgctcggc tggtcgttct tctacttcag cgacgacacc cgcggtttca agccggtcga
    10801 cacgtcggtg gccgtcgcgc agatcaactc cgacaacgtc aagaacgccc agatcgacga
    10861 ccgcgagcag caactgcggc tgacgctgaa gaacggcaac gaccagaccg gcggcagcga
    10921 caagatcatc acgaagtacc cgactggcta cgccgccacg ctgttcgacc agctcaccac
    10981 caagaacgcg ccggtcaaca ccgtcgtgaa ccagggcaac atgctcgggt cgctgttcat
    11041 atatctgctg ccgctgctgc tgctcgtcgg cctgtttgtg atgttctccc ggatgcaggg
    11101 cggcagccgg atgggcttcg ggtttggcaa gtcgcgtgcc aagcagctct ccaaggacat
    11161 gcccaagacc acgttcgacg acgtggccgg cgtcgacgag gccgtcgaag agctctacga
    11221 gatcaaggac ttcttggcca acccgacgcg gtatcaagcc ctgggcgcca agatccccaa
    11281 gggggtgctg ctctacggcc cacccggcac cggcaagacg ctgctggcgc gtgccgtcgc
    11341 cggcgaggcg gccgtgccgt tcttcacgat ctccggctcc gacttcgtcg agatgttcgt
    11401 cggcgtcggc gcatcccggg tgcgcgacct gttcgagcag gccaaacaga acagcccgtg
    11461 catcatcttc gtcgacgaga tcgacgccgt cggccgtcag cgcggcgcgg gcctgggcgg
    11521 cggccacgac gaacgcgaac agacgctcaa ccagctgctg gtcgagatgg acggcttcgg
    11581 cgaccgcacc ggcgtcatcc tgatcgccgc caccaaccgg cccgacatcc tcgacccggc
    11641 gctgctccgg ccgggccgct tcgaccgcca gatcccggtg accaacccag atctcaacgg
    11701 gcgcaaggcg attctccggg tgcactcggc cggcaagccg atggccccca acgccgacct
    11761 cgacgggctg gccaaacgga ccgtcggcat gtccggcgcc gacctcgcca acgtcgtcaa
    11821 cgaggccgcg ctgctcaccg cgcgcgagaa cggcacggtc atcaccggcg ccgcgctcga
    11881 agaggcagtg gaccgggtga tcggcggccc ccgccgcaag agccgcgtga tcagcgagct
    11941 ggaaaagaag atcaccgcct accacgaggg tggccacacg ctggcggcct gggcgatgcc
    12001 cgacatcgag ccgatctaca aggtgacgat cctggcccgc ggccgcaccg gcggccacgc
    12061 cgtgtccgtc ccggaagacg acaagggcat gatgacccgc tcggagatga tcgcgcggct
    12121 ggtgttcgcg atgggcggtc gagccgccga ggagttggtg tttcgcgagc cgaccaccgg
    12181 cgcggtgtcc gatatcgacc aggccaccaa gatcgcccgc gcgatggtca ccgagtacgg
    12241 aatgagttcc aagctaggcg ctgtcaagta cggcaccgaa cacggcgacc cgttcctcgg
    12301 ccgcacgatg ggcacccagt ccgattacag ccacgaggtc gcccgcgaca tcgacgacga
    12361 ggtccgcaag ctgatcgagg ctgcgcacac cgaggcgtgg gagatcctca ccgagtatcg
    12421 cgacgtgctg gacacgctgg ccagcgagct actggagaag gagaccctgc accgggccga
    12481 gctggaagcc atcttcggcg gagtgcagaa gcggccgcgt ctcaccgtct tcgacgactt
    12541 cggtggccgc atcccgtcgg acaagccgcc gatcaagacg cccggcgagc tcgcgatgga
    12601 acggggcgag ccgtggccgc agcccgtccc cgagcccgcg ttcaagcagg cgatcgccca
    12661 ggccagcgag gaggcggagg cggctcgcga cgcgagcggc aacggcaacg ggtccaacgg
    12721 ccacccggcc cagccgcagc ccgttccggc ggccacgcag ccggactacg gcgcgccggc
    12781 gggatggcgg gcgcccggct ggccgccgca gccgccgcag gctcactggc cgccgcagcc
    12841 gccgcaggct cagtggtatc cacccccgca gacgtacggg ccgtaccaac agaatccgca
    12901 gggccagtca caatcggaag gtgcacctga caggcaggag cgcgacgagc ggggtcagga
    12961 acctggccgt gccggcccgt ccacccaggg ctgactgacg aacggaggcg ttgatggccc
    13021 agccgaattc gcgcgtttcg agaatcggcg tattcgacag ggagcgagcc gaggccgcgg
    13081 tccgcgagct gctcctcgca atcggtgaag accccgaccg gcacgggctg cgtgatacgc
    13141 ccgcccgggt ggcacgggct taccacgaga tgttcgccgg cctgtacatc gaccccgact
    13201 ccgttctcaa caccactttc gacgaacagc acgacgagtt gatcctggtc aaggagatcc
    13261 cgatgtactc gacctgcgaa caccacctgg tgtcgttcca cggcgtcgcg cacgtcgggt
    13321 atatcccggg cgaggacggc cgggtgaccg ggctgtcgaa gatcgcccgg gtggtcgacc
    13381 tgtacgccaa gcgcccccag ttgcaagagc ggctcaccgg gcagatcgcc gacgcattga
    13441 tgcgcaagct cgacccgcgc ggcgtgatcg tggtcgtgga ggccgagcac ctgtgcatgg
    13501 cgatgcgagg ggtgcgcaaa cccggcgcgg tcacaacgac ttccgcggtc cgcgggcagt
    13561 tcaagaccga caatgcatcg cgggccgagg cgctggatct gatcttgcgg aagtgagcac
    13621 gacgcgcgtg caggtgatgg gcgtcgtcaa cgtcaccgac gactccttct ccgacggggg
    13681 gctgtacctc gaccgggacc gtgcggtcga gcacgggcag gcgctcgcgg ccgacggcgc
    13741 cgcgatcgtc gacgtgggcg gcgagtccac tcgacccggc gccaccagag tcgacccgcg
    13801 agtcgaaacc gatcgcgtcg taccggtcgt taaagccctt gccgcacaag ggatcaccgt
    13861 cagtatcgat acgatgcggg ccgaggtcgc ggccgcggcg ctgcagcacg gcgcgcggat
    13921 ggtcaacgac gtctccgggg gccgcgccga cccgaagatg gcgccgctgg tggccgacgc
    13981 cgacgtgccc tggatcctga tgcactggcg gtcggtgagc gccgcgcgac ctcacgacgt
    14041 gcccgactac ggtgacgtcg tcgccgacgt caaggcggag ctgcttgcca gcgtcgacgc
    14101 cgcaacgtcg gcgggcgtgg acgcgacgcg gttgatcatc gaccccggtc tgggtttcgc
    14161 caagacggcg caacacaatt gggcactgct gcgggcgctg ccggagctcg tcgccaccgg
    14221 gattccggtg ctggtaggtg cgtcgcgcaa gcgcttcctg gggtcgctgc tggccgacga
    14281 gcacggtgat cgcccgccgg caggccgcga aacggcgacg gcggtggtct ccgcgctggc
    14341 cgcattgcag ggagtgtggg gtgttcgggt gcacgacgtg cgggcgaccg tcgacgcgtt
    14401 gaaggtggtc gaggcctgga aggcaggcct gcgcggtgac tgaccgaatc ttgttgcacg
    14461 gcttgactgt tcgcggtcat cacggcgtgt tcgagcacga acgccgtgat gggcaggagt
    14521 tcgtcgtcga catcacgttg tggatcgacc tcgaccgtgc cggcgcgagc gacgatcttg
    14581 cggacaccat cgactacggc gaattggcca agcgggcagc cgacattgtc ggcgggccgc
    14641 ccagaaacct gatcgaagcg gtggccgcag acatcgccga cgacgtcatg cgcgacgaga
    14701 gggtgcacgc cgtcgaggtg acgatccaca aaccccaggc accaatccct ttggcgttca
    14761 ccgacgttgc tgttgtcgcc cgcaggtcac gccgttccgg gcgggtgacc gggtgacccg
    14821 cgcggtgctc tcgatcgggt cgaatctggg cgaccgactg gcgcggctgc aatcagtggt
    14881 cgacgggctg ggcgactcgg tggtcgcggt gtcgcccgtg tacgagacgg tgccgtgggg
    14941 tggggtagag cagggcccgt tcctgaacgc ggtgctgatc gcagacgacc ccgacttgga
    15001 cggcctcggc tggttgcgcc tcggccagaa gctggaaaag gccgcagacc gcgtgcgcgg
    15061 tgagcgctgg ggtccccgca ccctcgacgt cgatctggtg agctgctccg ccgacgggcg
    15121 agagcttgtt gtccgcgaac ccgacctgac cttgccgcac ccgctcgcgc acctgcgtgc
    15181 gttcgtattg gtgccgtggc tggcgatcga cccggaggcc gagttgatga tcgccgaggt
    15241 gtcgacgccg gtgacccagc tgcttgacga catcgagcct gtcgacaagg ccggtgtcat
    15301 gcccacggac ctgaccctgc ggtcgacggt gtgacatggg gccgacccgc aagcgtgacc
    15361 tgacggccgc ggtggtcgcc gttgcggtgg tgggctactt cgtgaccgcg gcggtctacc
    15421 ccggctggtt tccgccgatc accgtctgga ccgggctctc tctgctgggg gtggccgcgg
    15481 tggaggccgc atggggccgc cacgtgcggt cgaagatcaa cgacgggatg atcggcgacg
    15541 gcggcggcag gctccacccg attgcggtcg cacgaacggt gacgatcgcg aaagcgtcgg
    15601 catgggtggg ggcgctgatg ctcgggtggt ggttcggcgt gctggcctac ctactgacca
    15661 accgatcctt gagcgtggcc gtcagtgaca cccccggcgc tgccgtcgcg gccgccagcg
    15721 cgctggccct gatcatcgcg gcgctgtggc ttcagcattg ctgcgagaag cccgaggagc
    15781 cgccggacag cccgggcgca accgctgaat agaatcgccg ggcagggtcg acacgcgacg
    15841 tgacctggac gggtacagtt ccgcccatga ccgatctgag ccgcggcggc cggtcacggc
    15901 gcagcgaccg caggccgggt tggctgcttc tttcggtgtt gctagtcctg gctatcgcgg
    15961 ccagttccgc gttggttttc accgagcgcg tcgagctgct gaagctcgcg gtgatcctgt
    16021 cgctatgggc ggccgcgacc gcagctttcg tctcggtcat ctaccgcagg cagagcgatc
    16081 tggaccgggc caaggcgcgc gacctcaagc tggtgtacga cctgcagctg gaccgcgaga
    16141 tctccgcccg ccgcgaatac gagctggagg tggagtcgca gctgcgccgg gagctggcct
    16201 ccgagctgcg ggcgcagtcc gccgaagagg tcgccgagct gcgggccgaa ctcgccgcgc
    16261 tgcgcaacaa cctcgagatc ctgttcgacg ccgacctctc acaccggccg gccatcgagc
    16321 cggagcgcac gacggtgcgc gcctacagcg actgggagcg cgacaccgag atcaccgacc
    16381 cagggcgaaa catcccgcgg tatgtcgagt ccacaccgcg ccagcacccc gacgacgaca
    16441 ccgagaacgc gatcatcgac gtcccggagg aacccccggt ccccgaagag gaagtgcgcg
    16501 tcgcgtcaga accccaccag ccggagcagg tgtggacgcc gccgcaaccc gagccggcgg
    16561 cacacgccga gcagccggca gagcgtggca gcaggcacgg gcttcgcgcc gaacgcgagt
    16621 ggcagcccgt tccctccgca gggcagtggt tgccgccggg cgccccgggc agtaactggg
    16681 tctccaccaa cggcacggcg ccgacgccgg ggtcggcccc gccaccgcca gtcccagccg
    16741 agcccggcct cgggcgacac tccaacccgg tcgagccggt cggggcgccg gccgcgcccc
    16801 cgccacaaca ttcgaccgag agcgtcggct atgcgccggc cccgcccccg ccacaacagc
    16861 ccccggccag gcacgacgac gcgggcgaca gcgcccctgg cgtaggtcag cacggcgagg
    16921 gtcaaggcca gtccgtcgct gagctgctgg cacgtttgca ggccggcaca ggcccgaccg
    16981 cgggcggccg tcgccggcgt cgcgacggtt aagctaccct ttggtggacc gtccggtacc
    17041 cgcctggcag gaccggaacg aattttcgaa acactttgcg aggtcgtctg cgatggagca
    17101 gtctcctacg cacgcgtggg gtccccccga cggactgcgc ccggctcggc tcttggtcgg
    17161 gatcatctcc gcgggccggg tcggcactgc gctcggctcg gcgctggagc gtgcgggaca
    17221 cgtcgtggtg gcctgcagcg gggtgtcgca cgcctccagg gagcgggcta gccgtcgtct
    17281 gcccgacagc gcgatcctcc cggcccacga ggtcgccgcc cgcggcgagt tgctgctgct
    17341 ggcggtgccc gacgccgaac tcgcgagcgt ggtgtcgggg ctggcggcca cccgcgcggt
    17401 gcgcccgggc acgatcgtcg tacacacgtc gggcgccaac ggcatcggcg tgctggcgcc
    17461 actgacggag caaggctgcc tcccgttggc gattcacccc gcgatgacgt tcaccggctc
    17521 cgacgaggac atcgcccggt tgccggacac gtgcttcggc atcaccgcgg ccgacgacgt
    17581 gggctacgcg atcgggcagt cgctggtcct cgaggtcggg ggtgaaccgt tccgggtgcg
    17641 tgaggatgcg cggacgctgt accacgccgc gctggcgcat gccagcaacc acgtcgtcac
    17701 cgtcgtcgcc gacgccctcg aagcgctgcg gacggcgctg tggggtcagg agctgctggg
    17761 tcagcaactc gtcgacgatg cgcccggagg cattgccgag cgggtgatcg gcccgctgac
    17821 ccgagcggcg gtcgagaaca cgctgcagcg gggtcaggca gctttgaccg gtccggttgc
    17881 gcgcggggac gccgacgccg tcgcggccca cctgcgcgcg ttggcggaag tggatccgcg
    17941 gctcgccgag gcgtaccgga ccggctcgct gcgcaccgcg cagcgggatc acgcctcgcc
    18001 ggaaatcttt gcggcgcttg ataattcacc ggatcggacg ggctgcggtg acaattaacc
    18061 tgaacaagaa gttccatccc ggacagctga acgtctacga gacgccgaag gctgtcgccg
    18121 aggtgacccg cgcgttgcgc caaaccggcc gccgggtcat gctggtgccg acgatgggag
    18181 cccttcacgc cggccacctt gcgctagtgc gagccgccag gcgggtgccc ggttcggtgg
    18241 tagtggtgtc gatcttcgtc aacccgctgc agttcggtgc cggcggggat ctcgacagtt
    18301 atccgcgcat gatggacgcc gacctggacc tgctgcgacg cgaaggcgtc gaagtggtgt
    18361 tcgctcccag cgtcgaggcg atgtaccccg acggcccccg cacgtcggtt cagccggggc
    18421 cgctcggtgc ggaactcgaa ggcgcatcgc ggccaacgca tttcggaggc atgctgacgg
    18481 tagttctgaa gctgctgcaa accgtcgctc ccgatcgggc attcttcggt gagaaggact
    18541 accagcagct ggcgctgatc cggcaaatgg tcgccgactt caacctggac gtgcgcgtgg
    18601 tgggcgtgcc gatcgtgcgg gaggccgacg gactcgcgct gtcgtcgcgc aaccgctacc
    18661 tcacgcccac cgaacgcgaa ctcgccgggg cgctctcggc ggcactgctg gccgggatgt
    18721 acgcggcatc cggtggagcg gacgccgctt tggacgcggc ccgcgccgtg ctcgccgagg
    18781 tgccggcgct cgaggtcgac tatctcgaaa tccgcgggcc cgcactggaa cccgcgcccg
    18841 cagctggtct cggccgcctg ctggtagccg cccgcctcgg cgacaccaga ctcatcgaca
    18901 acatcgccat cgatatcggc ggacccgacg ggcagtcgtc cgacgggtcg gacgatcatc
    18961 acgaactgcc ttggaggaac tgatgttacg cacgatgctg aagtcgaaga tccatcgcgc
    19021 caccgtcacc gaggccgact tgcgctacgt cggatcggtc accatcgacg ccgacttgat
    19081 ggacgccgca gatctgctcg aaggcgagca ggtgaccatc gtcgacgtga ctaacggcgc
    19141 gcggctggtc acatacgcga tcaccggcaa gcgaggcagc ggtgtcatcg ggatcaacgg
    19201 ggccgctgcc catctcgtgc atccgggcga catcgtgata ttgatcgcct acggtacgat
    19261 gaccgacgcc gaagcgcgcg aataccggcc gcggatcgtg ttcgtcgacg ccgacaacaa
    19321 acaggtcgac ctcggcgccg acccggcgtt cgtccccgac cagtactcag gcctgatgtc
    19381 gctctccacg ggctccaacc tcgtgtcgcc gcggtagcca tgcttctcgc gatcgacgtc
    19441 cgcaacacgc acaccgtggt cggtttgatc acagggtctg gtgtgcattc gaaggtggtt
    19501 cagcactggc ggattcgcac cgagtcgcag gcgacctccg acgagatggc cctgaccatc
    19561 gacgggctga tcggagacga ctccggacag ctcaccggtg cggtcgccct gtcgacagtg
    19621 ccgtcggtct tgcacgagct gcgtgagatg ctcgaccaat actggccccg ggtgccgcac
    19681 gtgctgatcg agccgggagt tcggacgggc attccgctgc tggtggacaa cccgaaggaa
    19741 gtcggcgccg accggatcgt caactgcctt gccgcacacc agaagtacgg caccgcggcg
    19801 atcgtggtcg atttcggttc gtcgatctgc gtcgacgtgg tgtcggcgaa gggcgaattc
    19861 cttggcggcg caatcgctcc cggcgtcgaa gtgtcgtccg acgcggccgc cgcacgatcg
    19921 gcagcgctgc gccgcgtgga gctgacccgc ccgcgcagcg tcatcggcaa gaacaccgtc
    19981 gaatgcatgc aggccggagc ggtcttcggg ttcgcgggtt tggtcgacgg gctggttcgg
    20041 cggatccgcg acgacgtcga tggtttcgcg ggcgacgacg tcactgtggt cgcgaccgga
    20101 cacatcgcgc cgctgctgct gcccgccatg cacacggtgc agcactacga ccgtcacctc
    20161 accttggatg ggttgcggct cgtgttcgag cgcaaccggg acagccagcg gggaaagctc
    20221 aggcaggcgc gttgacgcgc gcccgcccgc taatgattgc tggatcgctg ttgcgcaaaa
    20281 ctcgctcccg cgttgtgagc gcgtgctagt ctccgcccga tgcgatctgg tgaacaggtg
    20341 tcgaggcagc gacgtgcgtc tggcgctgat gtcgttgctg gtgatccagg gtgcgggtat
    20401 gcagtcaccg agacccggaa caacggacgg gcccacgaga tttgttggtg cccaacagtg
    20461 ccgtgcgacg agggtagcga tcagctgctc attacacggt tggatcaact cagcaaaggc
    20521 tgaacatgaa tggcctgaaa tcgcaagcac aaagctctca ccgacgtctg tcccgtcgac
    20581 cactacccac gtggtcggtc gcgaccttgg tcgcggcgac agttttcgca acggtcgtga
    20641 cggggtgcgg gtctaagtca tcgacaccgt ccgagacgac tggggcatcc ggcaccaccg
    20701 cgacgagcgc gacggccacg acccccggcg agccgttgcc tgaggcctca aagatcctgc
    20761 aggcgagcgc caagacgacg cagacactgc attctgtcca catcgctttg accacaacga
    20821 atctgccgga tctcccgatg gagaccgtca acgccgacgt gaccaatctg ccccagggca
    20881 gcgcccgggc agtcggcgat gccaaggtcc gaacccagcc gaacgccccg gtcaatgagt
    20941 tttcgttcgt ggtcacggac aagacgctgt acacgaaggg ggctgacggt aaatacacgt
    21001 cgatgggccc ggcccagaag atctacgacc cgggcatcgt cctttccaag gacaagggac
    21061 tggcccacct catcgagcag gtgcagaacc ccaaggtcga cgggcgcgaa acgatcaacg
    21121 gcattgtcac cattaaggtg tccggaacca tcgatgctgc tgtgatcgat ccgattgtgc
    21181 cgcagttagg aaaggatgga ggcacactcc cgatcacctt gtggatcgcc gatgtacctg
    21241 cctccgcgac accgaccacg actcttccgt cagacgcgcc gtcacccggt accggcccga
    21301 acctggtgcg gttcatggtc aataaggacc cgggcaacgt tgaggtgacg ttgtcgaatt
    21361 gggcgaagcc agtcgacatc cccaacccca cggggtaaga ggcacgagcg tgagttccgc
    21421 atcgggaatc ggcctgggcg tgcggtacgg cggcaatggc cgccggccgt ccccgggccg
    21481 gttctcgtcc tcaggtgatg tccgtcaaca ccgctatggc gcacccttcc tgcccttgtt
    21541 cggacctcaa gggctgcagt tgaggaggat gccaccgttc ccctggttcg ctgacctgcg
    21601 acccgccgag tggaacgcga tgccgaagct actgaattaa gcgagtgttc agtgaagccc
    21661 gcggcgctgc gaagacgaaa tggtccgaat gcgcctgagg gtgcaaaggg tctggcgcaa
    21721 cttcatctca cctgctttcg tgctccggtg gcgagcatcg gtcgccgcag tcgtcaagcc
    21781 gcacagcgcg gtcatttcgc accgggattc agcatgcgcc atgctgccgt ctgctgtaga
    21841 ggtagtcgag atgccaccaa gaatctgttg agaatccgcc aaggcggtga cccgacgctg
    21901 ggtcaacgca gggagcgcag cgtatcgcga ggacccaacc cgagaagacc cgaaagaatg
    21961 acatgaccaa cgacccgcca caagtacagg agcgtgacct cccacaagca cgggagcgcg
    22021 actcgcgtcg acccccaagg cgcgcgggcg gtccggcgaa tgtatgggtc tacaagggga
    22081 aagcctacga tctgaccgat tggatttcga agcatcccgg cggcgccttc ttcatcgggc
    22141 gaacgagggg tcgcgacatc acttccatca tcgggtcgta ccatcgcaac cccgcggcga
    22201 tcgagagggt gctggagcgg tacgcgctcg gccgcgacgc aacccctgag gacatccacc
    22261 cgaagaacaa tgcaccgcct ttcctgttca aggacgattt caacagctgg cgggacaccc
    22321 caaagttccg attcgacgac aagaacgatc tactccaccg ggtgaagacg cgactgaagg
    22381 accgtcagct cgcaacgcgc atccgaaaga tggacacggt attcaacatc gtggtcgggc
    22441 tgctggccgt tgcgtacttg gcggttcagg gtctacggct ggctgaaccc gcttggatgc
    22501 cgttgccgct cttcgtgatc gcgatggtcc tacttcgcag ttctctggtc gggttcggtc
    22561 attacgcgat acatcgcgcg caaaaaggcg tcactaggtt cttgtcgagt tcctttgacc
    22621 tgaactacgt ggcgttggcc ttggtcgtcg ccgacggaca tactcttctc caccatccgc
    22681 acacccaaag tgaagtcgac atcaagaaga acgtcttcac gatgatgatg cggctgcccc
    22741 ggctgtaccg gatacccgtg cacactgttc acaagttcgg gcacctggtc agcggcatgg
    22801 ccatccgcat agtcgacgtc gtcaagatca cgcgcaaggt tggggtcgag aagctttacg
    22861 gaagctggcg caacgcgctt gctcacttga tcggctcgtc gggtgtgcgc cttctcctca
    22921 tcggcgagct ggtggccttc atcttggccg gtgacttctg ggcctgggca ctgcaattcg
    22981 tcgcgacact ctgggtcagc accctcctga tcgtcgcgag ccacgacttc gaggaagacg
    23041 ccgacgaggt tcagtccgac accgaagact ggggcatcca ccaactcgag gagtcctacg
    23101 acctgaaggt gatcgggaac cgttatgtcg actgcttcct gtcggccggc ctgagttcgc
    23161 atcgcgtgca tcacgtgctg ccatatcagc gcagcgggtt tgcaaacatc gccaccgagg
    23221 acgtgttgcg ggaagaggcg gcgaagttcg gtgtcgagtg gcttcccgca aagagtttcg
    23281 tcaccgatcg gcttccgaag ctctgccgaa cgtacctctt gtcacgttcc cgccaagccg
    23341 cggaacagaa ttggggcttc gtccgcgagc acttctcgcc cgcggccttc aaaaccagcg
    23401 ccaactacgt ggccggggga ttcgccggac tcggttcggt gtgaggggag gacgtcgtga
    23461 ctgccatttt cgatgaagcc gaaacgtgcg gcccggccgg tgaagcggtg ggtcttctcg
    23521 ctgaattgcc gccgtcgccg ccgacgacgg tggcggtgat cgaaagtatc gccacgggtg
    23581 caccgggtcg gatggtggat cagtgcggcg ctgctgaccg agtagccgca ttgttcgcag
    23641 aggatccaca gcagcgggcg cggattgcgc gggtctatcg aaagaccagg atcgccagcc
    23701 gccagatggc ggtggatcca ctggatgccg agttcgatgg ggttcggcgg gagccggcca
    23761 tggtccgtaa tcggatgaat ctgttttacc agcatgctgt tccgttggca gtggacgtgg
    23821 ccgggcgtgc tctggccggt ggtggacacg tggcatccga aatcggattg ttggtgtttg
    23881 tgacgagcac tgggttcatc gctccggggg tggacgtggc ggtggtcaag cagttggggt
    23941 tgtcaccatc ggtgtcgcgc gtggtggtga acttcatggg gtgcgcggcc gcgatgaacg
    24001 gcatccgcac ggctgccgac catgttcgcg cccacccgga cactaaggcg ttggtggtgt
    24061 gcatcgaatt atgttcggtc aacgcggttt tcgccgatga catcaacgac gtcatcattt
    24121 ccagcttgtt cggggacggg tgcgcggccg tggtcgtcgg cgccagcccg gtcaagcatc
    24181 cgttggcgcc gggcaaggtg gtgatccgca ggagcttcag caggctgctc gatgacgccg
    24241 aagacggcat cgtgctggga gtcaatcaca acggcatcac ctgcgagttg tcggagaacc
    24301 ttcccggcta catctacgac ggcgtggatc ctgtggtaac agaggtgttg gctgacaacg
    24361 gattacgcaa gtccgacgtc gacttgtggg cgatccatcc gggcggacca aaaatcatcg
    24421 aggaatcggt gcggtctttg ggcattcccg ccgacgcagc ggcggcgagt tgggaggtgc
    24481 tggcccggta cggcaacatg ctcagcgtat cgctgatctt tgtgctggaa gtgatggtgg
    24541 ccaacgcacg atcgaccaag gctatctcca ctggagtggc gttctcgttt gcgccgggcg
    24601 tggcggtcga gggaatgatg ttcgacatca tccggcactg atcgcgacga acgacgaagg
    24661 cgcaaaacga tgcaactcat caaattcttg atttcgatct cgccggggcg gatcgcggcc
    24721 gtgttcgtcg ccggcttggt cggcggggtg gccaacacct acctgctgac cctcatcggc
    24781 agtgtggtgt cgccggcggc ccaccacggc gccacgctgc agcgctttgc gataaccggg
    24841 ttgatcgcgc ttgtcagtgc cgtgctctcg cagacgctgc tcatccgcct cacccaggat
    24901 gcgatctatc acttgcgtgc gaagttctgc tcggaggtgg tcgcgctccc gctggagcat
    24961 ctggaacggc tgggcatgca ccgactgctg gggactctga ccgaggatgt gcggtcgctg
    25021 tctcaggcgg tgacggccat tccgaatgtc tgtgtcgccg cggtgaccat cgtgggttgt
    25081 ctggcgttcc tggcgctggt gtcgggcccg attttcgctg tcacggtggc cggcttggcg
    25141 gtgggcactc ttggcgttga ggcgttgtcg aggagagtgc gacaccttta caccgaggcc
    25201 cgcgacaacg aggacgcact gctgcggtca tttcaatcgg tgacgtcagg catcaaggaa
    25261 ctcaagctgc accgtggccg gcgccgcgac ttcatggatc gccacctgct cggcacggcc
    25321 ggcgtcctaa gggatcagaa cgtggccgcc ggttcccgaa tctcgctggc gaacagctat
    25381 gggcggctgc ttcaactggt caccatggcc gccattttgt tcgtgctcgc caggacgctc
    25441 gatgtgccgc gctcggtcat ggtcacctac atcctgatca ccatgtatat gtcggtgccg
    25501 acgcagaatt tcatgcagcg catccccgac ctgcggcgcg gtaacgtcgc gctcgccaag
    25561 atccgcgcga tggacctgtc aatgcagact aaacacgatg agcagcacct gccccactcc
    25621 gatcggccca ccgccaccac agcccggctg gaactgacca acctcagcta cacgtacaag
    25681 gctgaggcgg ccccgccgtt tccgaaaggt cctaccgcag tgggaaatcc gccgcgaggt
    25741 ggcccacatt ctgggcgtca cggtcccggc gggaagatgg cgggtcggcc gccgggtccg
    25801 cttgccggtg tggacgctgg cgaatcaggc ttccggctgg gtccgctgga tctcttgttt
    25861 gaacccgggc aaatcagcta cgtcgtcggg ggcaatggta gcggcaaatc caccctggcc
    25921 aagctgctga ccgggctgta ttcgccagag actgggttcg tgacgctcaa cggcgaaaag
    25981 atcggccacg acaacatcga ctggttccgg cagaactgct ctgcggtctt taccgacttt
    26041 catctgttcg aggagtatct gggcttcgtc cggcccggga tcgatgacga ggtgcgccac
    26101 tacctggccg aactgcaact ggcgcataag gtttcggtgc aggatgggcg gctgtcgacg
    26161 gtggcgctgt cacaaggcca acgcaaacgg ctggcgttgt tgaccgcgct gctggaggac
    26221 cggccgatct atgtgttcga cgagtgggcc gccgatcagg agccacggtt ccgcgacgtg
    26281 ttctacaccg agatcctgac cgggctggcc cggcgcggca agaccgtcat cgtcatcacc
    26341 cacgacgacc gctacttcca ctgcgccgat cagatcgtca aactcgacta cggccacatc
    26401 gaagacatca cttcaccagc gccggtccga cctgcgacgc cacggcgccc tgccgcttct
    26461 taggaacgtc gacgagccac gagtcgccgg ctcgtgtcgc tcgcgggttg tcctgtcatg
    26521 caggctcttc cgtggtatac cgtgcgccga ccaggttgtg cagtgtcatc ggttccagtg
    26581 cgcgccgatg gaacaccaag aatccgttga ggattcgtca aggcggctat ccgattgtga
    26641 ggacagtgca cgagtaagtg ctgccagctg atcgatctca taccaatcca tggcagcacc
    26701 gctcatcgtt ctggaaggga gacgtctcgt gtctgtgttt gataggggcc gcaatcggcg
    26761 cccggccgca gggtcattat gcgaccatgc cttcgtgacc ggtcgagtcg agtacctgtc
    26821 gtggatgagt gacaaccggc ggtgggacgc gctcgagttg cgtgagggcg acatcgtcat
    26881 ctctgcgccg ccgaagtgcg gcaacacctg gacacagcga ctcgtgtctc tgctcgtgtt
    26941 cgacgggccg gacctgccag ggccgctgtc gaaagtgtcg ccgtggctcg accagaccac
    27001 catgcctgtc gaggaggttg tcgccgcact caaggcccag ggacaccgcc ggttcatcaa
    27061 gacccacaca cccctcgacg ggctcgtcct cgacgatcgc gtcacgtaca tcggactagg
    27121 gcgcgacccg cgtgatgccg tgatgtcgat gatcatggac tgggaccgga tgcgggcgct
    27181 ccgccacgcc acgatgccgc cgaacgagcg ggtcggcccg gcgggtccgc gcccccgccg
    27241 cgtcagcccg ctcgaggcgt tccaggaatg gatggactgg cctgccatgc ctactgtggg
    27301 aggtcggccc tcgactttga ggccaggcgc cgctgagcct cggtctggga tctgggtgtc
    27361 actggctaat ttccttcacc acttcgactc attctggtct ctgcgccacc tgcccaatgt
    27421 ggcactgttc cactacgccg actttcaatt ggacctcgcc ggcgaggtcg tccgtctcgg
    27481 tcaggtcctc gatctcgatg tgggtcgtga tcgcgccgag gagctggcca agcacggcac
    27541 attagatgcc atgcgggctc gcgccgcgga attcgcgccc gatgcggacc ggggcatccg
    27601 gcgcggcgac tcccccttct tccgggccgg tggtcgcggc acgtggcggg acatcgccac
    27661 cgaggacgtt ctccggcgct acaaccagcg cgccgggcgg ctagcctcgc cggacctcct
    27721 cgcctgggct catgagggcc gacggggcca cgagcccgac aacgcgacgt cgccctagaa
    27781 gaaggtgcgg atcaagtcga ccacccgtcc atcgctgtcg acaaccggaa tcagctgcca
    27841 cttgtcgaac accgtgcacg ggtgcgagat gccgaactcc agccacgtgc ccacgtcgat
    27901 gcgcgatcgg tcggcggggc cgacgtgcag gtatgcgtgc tggtcgttca ggcggctgat
    27961 gcggctgtcg ggaagcgcga ggggcgtcgg aaggtcctgg tcgaacgaca cgtcgcgtcg
    28021 gccaatcgtc agaaccgcca actcgggctc cgggatcgat gtcacctggg cccagacgct
    28081 catcgcgggc catagaccat gacggcccgt gcgggtcagc ggcgacgtgc gctggtacag
    28141 cccgtggtca ttcgtcagat aggcaccgct gcgaatcacc gtccgcacct gaagcccgga
    28201 aggccacccg gcagccagca cgtctgccac gagatcgaag tacgtgcttc cgccggccgt
    28261 caccagaacc tcgtcggatt cgaacgccag ccgctgcacg gccgaacgta tgtcacccag
    28321 aaactcggtg accgaggaca acgcctcgac gctcacgtcg tggcctagcg ctgcctcgta
    28381 tccggcgacg ccgaccagac gcaagtacgg gcaggccacg accgcccgcg ccacgtcgtc
    28441 gaccgctgct gcacttcggc atccggtgcg cccgcctgcc atcccaactt ccacgcacac
    28501 gtcgactggg cgggtcgcgc aagtcgccaa ctcggccgcc atggtttgca caccgggcac
    28561 cgagtccacc cagcagacga agccaaattc cgggtcgcgg tcgagttcgc ctgccagcca
    28621 acgtaatccg cccgggtcga caagttggtt ggccaggatc acgtcgcgca caccgaatcc
    28681 gcggcacacc ctcacctggc tgaccgtcgc cgcggtgatg ccgcatgcac cggcgtcgag
    28741 ctggcgggcg aacagctgcg gtgccatgtg cgtcttgccg tgcggcgcaa tctccacacc
    28801 cgcgtcgcgg caccagttcg ccatcgtcgt caggttgtgg gtcaacgcgt cggcgtccag
    28861 cacgcagacc ggtccggtcg gtccgtcgtc gaacagcgcc ggccggtcgg cgcagatctg
    28921 cctaggggta cggccggtcc acgacggcgg cagccccttg aaccgccagt ccaccggctc
    28981 gtcggccagc gcggccactg tagttcggtc gagcacctat tcatctaagc tggccagtcg
    29041 tgagttcagc cgacccgtgc gagcccgacg ttccggaaca gttccggatc cggcagggca
    29101 agcgcgagcg actgctcgat gagggcaacg acccgtatcc ggtgaatgtc cccgtcaccc
    29161 acacgctcaa gcagatccgc gagaactacc ccgacctgcc cgccgacgcc accaccggcg
    29221 agatcgtcgg cgtcgcgggc cgtgtggtgt tcgcccgcaa ctcgggcaag ctgtgcttcg
    29281 ccacgctgca gcaaggcgac ggtacccagc tgcaggtgat gatcagcctg gccagcgtcg
    29341 ggcaggaggt gctggaccga tggaagtccg aggtcgacct cggtgacatc gtgttcgtgc
    29401 acggcgaggt gatcagctcg cgccgcggtg agctgtctgt gctggccgac gcctggcgaa
    29461 tggcggctaa ggcactgcgg ccgctgccgg tggcgcacag ggagatgacc gaggagtccc
    29521 gggtacggca gcgctatgtc gacctgatca tgcggcccga ggcgcgggcg gtggctcgcc
    29581 agcgggtcgc cgtggtgcgc gcgctgcgaa acgccttgca ccggcgcgac ttccttgagg
    29641 tcgagtcacc catgctgcag acgctggcgg gcggggccgc cgcccgcccg ttcgtcaccc
    29701 attccaacgc gatggactcc gacctgttct tgcgcatagc cccagagctg ttcctgaagc
    29761 gctgcgtggt cggcggattc gacagggtct tcgagctgtc ccgggtgttt cgaaacgagg
    29821 gcgctgattc aacgcattcg ccggaatttt cgatgctgga gacttaccag gcatacggca
    29881 cctacgacga ttcggcggtg atgatccggg aagttattca ggaagtcgcc gacgatgtgt
    29941 tcggcacccg tcagttgacg ctcgccgacg ggactcggta tgacatcgac gggcaatggg
    30001 tgtcgatcga aatgtacccg tcgttgtctg aggcgctggg cgaacctatt actcctgata
    30061 catcggtaca gcaccttgtc agcatcgcaa ctcgactcgg gatcgagatc ccgactgatc
    30121 ggccgttcgg ccacggcaag ctgatcgaag aattatggga acattcggtc ggcaggaact
    30181 tgtatgcccc gacgttcgtg cgcgattacc cggtggaaac cacaccgttg acgcgccagc
    30241 accgcagcat ccccggcgtc accgagaagt gggacctcta cgtccgcgga atcgaattgg
    30301 ccaccggtta ttcggaattg atcgacccgg tggttcagcg cgagcgattc gaagcccagg
    30361 cccgcgccgc ggcggcgggt gacgacgagg caatggtcct cgacgaggat ttcctcaccg
    30421 cgatggagta tgccatgccg ccgaccaccg gcaccggaat gggcatcgac cggctgatga
    30481 tggcattgac ggggttggga attagagaga cggtattgtt cccgattgtt cgtcgacagg
    30541 ccaactgaac tgtgcgtctc gcaacgaact ggcgttggct aaccatggcg gtttatggca
    30601 gattgatagg tcggagtaga cttccgcgtc accggcgtga ggccgcaatg ttcagaagga
    30661 gcggtgtaga aaatggccaa gaaagtaacc gtgacgttgg tcgatgattt cgacggcgaa
    30721 gcagcggctg acgaaaccgt ggaatttggc ctcgacgggg tgacctacga gatagatctg
    30781 tcgtcgaaaa atgccaagaa gcttcgcgat gatctgaagg tttgggtgga aaagggccga
    30841 cgggtcggcg gtcgtcgccg cggccgcgga agctccgggc gtggccgtgc gtcgatcgac
    30901 cgtgagcaga gcgctgcgat ccgggagtgg gcgcgccgca acgggcacaa cgtgtcgacc
    30961 cgaggccgca tcccggctga cgtgatcgat gcgttccacg ctgcgacgta gccccggtgt
    31021 gatctgactc tccttgagcg cgagccgtcc ggtgggattt cgctagcggc gaaccgcacc
    31081 tggcggtacc gggaaacgaa ttggcgtttg acggcgttac aacccctgta acgagagacg
    31141 caccgcaaaa cggctgatgt gaggccgggc cggctggctc cggtggtggg gcagccaggg
    31201 cagcaggcta ttagagtgga tagcgggtta tgcgggttca actcgtcgat ccgcgtactc
    31261 actggtgagg gagagcaggt aaccaccgat gttcgaacga tttactgacc gcgcacgccg
    31321 cgttgtcgtc ttggcccagg aagaggccag gatgctcaac cacaactaca tcgggaccga
    31381 gcacatcctt ctcggattga tccacgaagg cgaaggcgtt gccgccaagt cgctggagtc
    31441 cctgggtatt tcgctcgagg gtgtccgcag ccaggtcgag gagatcatcg gccagggcca
    31501 gcaggcgccg tcgggtcaca tcccgttcac gccgcgtgcg aagaaggtct tggagctctc
    31561 cctgcgtgag gcgctgcagc tcgggcacaa ctacatcggc accgagcaca tcctgctcgg
    31621 gctgatccgc gagggcgagg gcgtggccgc ccaggttctg gtcaagctcg gcgccgagct
    31681 gacgcgggtg cgccagcaag tgatccagct gctgagcggc taccagggca aggagaccgc
    31741 cgaggccggc accggcgggc gcggcggcga atccggcaac ccgtccacgt cgctggtgct
    31801 cgaccagttc ggccgcaacc tcaccgcggc cgcgatggag ggcaagctcg acccggtcat
    31861 cggccgcgaa aaggaaatcg agcgggtgat gcaggtgttg agccgccgca ccaagaacaa
    31921 cccggtgctg atcggggagc ccggcgtcgg caagaccgcc gtcgtcgagg gtttggcgca
    31981 ggcgatcgtg cacggcgagg tccccgagac gttgaaggac aagcagctct acaccttgga
    32041 cctgggctcg ctggtggccg gctcgcgcta ccgcggtgac ttcgaggaac gcctgaagaa
    32101 ggtgctcaag gagatcaaca cccgcggcga catcatcctg ttcatcgacg agctgcacac
    32161 gctcgtcggt gccggcgccg ccgaaggcgc gatcgacgct gcgagcatct tgaagccgaa
    32221 gctggcccgc ggcgagctgc agaccatcgg cgccaccacg ctcgacgagt accgcaagta
    32281 catcgaaaag gatgccgcgc tggagcgccg cttccagccg gtgcaagtcg gcgagccgac
    32341 agtcccgcac acgatcgaga tcctgaaggg tctgcgcgac cgctacgagg cgcaccaccg
    32401 cgtgtcgatc accgacagcg cgatagtggc cgccgcgacg ctggccgacc gctacatcaa
    32461 cgaccggttc ctgccggaca aggccatcga cctgatcgac gaggccggcg cccggatgcg
    32521 gatccgccgg atgaccgcgc cgccagatct gcgcgagttc gacgagaaga tcgccgaggc
    32581 acgccgcgag aaggaatccg cgatcgacgc gcaggacttc gagaaggccg cttcgttgcg
    32641 cgaccgtgag aagacgctcg tcgcacagcg cgccgagcgt gaaaagcaat ggcgctcagg
    32701 cgatctcgac gtggttgccg aggtcgacga tgagcagatc gccgaggtgc tgggcaactg
    32761 gaccggcatc cccgtgttca agctgaccga ggaggagacc actcggctgc tgcgcatgga
    32821 ggaggagatc caccagcgga tcatcggcca ggaggatgcc gtccaggccg tctcgaaggc
    32881 gatccgccgc acccgcgccg gcctgaagga ccccaagcgc ccgtccggct cgttcatctt
    32941 cgccggcccg tccggtgtcg gtaagacaga gctgtcgaag gcgctggcca acttcttgtt
    33001 cggcgacgac gacgcgctga tccagatcga catgggcgag ttccacgacc ggttcaccgc
    33061 ctcgcggctg ttcggtgccc ctccgggcta cgtcggctac gaggagggcg ggcagctcac
    33121 cgagaaggtg cggcgcaagc cgttcagtgt ggtgctgttc gacgagatcg agaaggccca
    33181 ccaggagatc tacaacagcc tgttgcaggt gctcgaggac ggccggctca ccgacggcca
    33241 aggccgcacg gtcgacttca agaacaccgt gttgatcttc acgtcgaacc tgggcacggc
    33301 cgacatctcc aagccggttg gcctgggctt cacccagggt ggcggcgaga acaactacga
    33361 gcggatgaag cagaaggtca acgacgagtt gaagaagcac ttccgcccgg agttcctcaa
    33421 ccgcatcgac gatgtcatcg tcttccacca gctgacaacc gagcagatcg tccagatggt
    33481 ggacctgatg atcgcccggg tggagaagca gctgaagagc aaggacatgg cgatgcagct
    33541 gaccgacaag gccaaggcgc tgttggccaa gcgcggattc gacccggtgc tgggtgcgcg
    33601 gccgctgcgc cggacgatcc agcgcgagat cgaggacgcg ctctc
//
LOCUS       DASWBF010000182         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1266284,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000182 DASWBF010000000
VERSION     DASWBF010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266284"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF874_06450"
     CDS             complement(<1..749)
                     /locus_tag="VF874_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175740.1"
                     /translation="MVMASMRPPIAEQLLLHRPSAYTVNLTDKRVVITGASSGIGEAA
                     AILFAQQGATVVAVARRQDLLDAVAQRITTDGGCAIALPCDLSDLDAVDEAVKTVEDR
                     LGGVDILVNNAARSIRRPLVESLDRWHDVERTMALNYYAPLRLIRGFAPGMLQRGDGH
                     IINVATWGVFSDASPLFAVYNASKAALSSVSRVIETEWSGRGVHSTTLYYPLVATPMI
                     APTKEYQGLPALTPNEAAEWMITAARHRPVR"
     gene            983..1192
                     /locus_tag="VF874_06455"
     CDS             983..1192
                     /locus_tag="VF874_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175741.1"
                     /translation="MPEHVALSAVNSVMDRAGLNAKAEVELSVKPYEAILEQMEAGSR
                     AEYRRSVAERTIRNLKRRFPLPMTN"
     gene            1195..1461
                     /locus_tag="VF874_06460"
     CDS             1195..1461
                     /locus_tag="VF874_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9175742.1"
                     /translation="MDAGEIDAEIGHGRKNDDADALSVGVAALTSRTLKTAETEAETT
                     ALRAVVEHRDDLVKTRTQTVNRLHVVLTNLIPGGAGRNLGADRC"
     gene            1592..2167
                     /locus_tag="VF874_06465"
     CDS             1592..2167
                     /locus_tag="VF874_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9175743.1"
                     /translation="MTASATTLTDLHGLGALTAGKILGRAGDVGRFRSAAAFASYTGT
                     APIEVSSGDVVRHRLSRAGDRQLKLLPARHGHHPSPPRHPWQGLLPEETIRRQESQRS
                     HAVSETAPLRPGISTAEPRRSQTERQAREDTRGRLYRLARPAETPHTGSSDKSLPGPA
                     PKPYNPAETELLDTERRQYRASASQQLKRGS"
BASE COUNT          406 a          724 c          714 g          352 t
ORIGIN      
        1 atccgcacgg ggcggtgtcg ggctgcggtg atcatccact ccgcggcttc gttcggggtc
       61 agcgcgggca agccctggta ctccttggtg ggagcgatca tcggtgtcgc gacgagcggg
      121 tagtacagcg tggtcgagtg cacaccgcgg cccgaccact cggtttcgat cacccggctc
      181 accgacgaca gcgccgcctt cgacgcgttg tagacggcga acagcggtga ggcgtcgctg
      241 aacacgcccc acgtcgcgac gttgatgatg tgcccgtcgc cgcgctgcag catgccgggc
      301 gcgaaccccc ggatgagccg cagcggcgcg taatagttca gcgccatcgt ccgttcgacg
      361 tcatgccagc ggtcgagcga ctccaccagc ggccggcgga tcgaacgggc ggcgttgttg
      421 acgaggatgt cgacgccgcc gaggcggtcc tccaccgtct tcaccgcttc gtcgaccgcg
      481 tcgagatcgg acaggtcgca cggcagtgcg atcgcgcagc cgccgtcggt ggtgatccgc
      541 tgggcgaccg cgtcgagcag gtcttgtctg cgtgcgacgg cgacgacggt ggcgccctgt
      601 tgcgcgaaca agatagcggc cgcttcgccg atgcccgacg acgcgccggt gatcacgacg
      661 cgcttgtcgg tcaggtttac ggtgtaggcc gacggccggt gcagcagtag ctgttcggcg
      721 atcggcggtc gcatgctcgc catcaccatc gcgcgggaga gccgccgcaa gggattcact
      781 cctcggagtg tatccggtga aatgcagggc acgaagctcg caaaccggcg agccgtgcaa
      841 acgctgggcc tgccacggtc aagtggtgtg tgctacgcat ggcgggcgtg ccccacaatc
      901 aaaagccgct gcgctcaggc gattacagta ggctcttgaa ccgctggcgg tgcgtcgtct
      961 cgggctggcg ctggattccg ttgtgccaga acacgtcgcg ctctcggcgg tgaactcggt
     1021 gatggaccgc gctggcctga acgcgaaggc cgaagtcgaa ttgagtgtga aaccctatga
     1081 ggcgattctg gaacagatgg aagccggatc tcgtgctgag tatcgcagat ctgtggcaga
     1141 gaggacgatt cggaatctca agcggcgctt cccgctgccg atgacgaact gatcatggac
     1201 gctggcgaga tcgacgccga gatcgggcac ggccgcaaga acgacgacgc cgatgcgttg
     1261 tcggtcggtg tcgccgcgct gacatcacgc acgctgaaaa ctgccgaaac cgaggccgaa
     1321 accacggcgt tgcgcgccgt cgtggagcac cgcgacgacc tggtgaagac tcgcacccag
     1381 accgtgaacc gactgcacgt ggtgctcacc aacctgattc ccggcggtgc cggacgcaac
     1441 ttgggcgcag atcgctgctg aactcctgcg cggcgtgcga ccccgcgatg ccgccggcaa
     1501 gacgctgcgt gccctggcgc taaatctggt cagtgaggtg cgccagctgg atcgccggat
     1561 cgccaaggcc gccagcgata ttcagaccgc tgtgaccgca tccgccacca ccttgaccga
     1621 cctgcacggc ctcggcgcat tgaccgcggg caagatcctc ggccgcgccg gtgacgttgg
     1681 ccggttccgg tcggcggccg cgttcgccag ctacaccggc accgccccga tcgaggtgtc
     1741 ctccggcgac gtggtccgtc accgactttc ccgcgccggc gatcgccaac tcaaacttct
     1801 gcctgcacgt catggccatc acccaagtcc gccaagacac ccctggcagg gcctactacc
     1861 tgaggaaacg atccgaaggc aagagtcaca acgaagccat gcggtgtctg aaacggcgcc
     1921 tctccgacct ggtatatcga cagctgaacc gcgacgcagc caaacggaaa ggcaggcccg
     1981 ggaggacact cgggggcggc tctatcgtct tgcgcggccg gctgaaaccc cgcacaccgg
     2041 ctcttcagac aagtcacttc ccgggcccgc tcccaaaccc tacaaccccg cagaaaccga
     2101 actacttgac acagagaggc gccagtatcg cgcatcagct tcacagcagc tcaaacgcgg
     2161 tagttagagc agcaaatagc ggtccgtgac ctagat
//
LOCUS       DASWBF010000183        15509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_216904,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000183 DASWBF010000000
VERSION     DASWBF010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15509
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216904"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(805..1839)
                     /locus_tag="VF874_06470"
     CDS             complement(805..1839)
                     /locus_tag="VF874_06470"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX9175744.1"
                     /translation="MMVAIGVVGATGQVGLVMRTLLEERDFPATSVRFFASSRSAGRK
                     LAFRGQEIEVEDAAIADPAGLDIALFSAGATMSRVQAPRFAAAGVTVIDNSSAWRKDP
                     DVPLVVSEVNFAAAANRPKGIIANPNCTTMAAMPVLKVLHDEAQLVRMVASTYQAVSG
                     SGVAGVDELAAQARAVIDDAEQLVHDGAALDFPPPEKYVAPIAFNVVPLAGSLVDDGS
                     GETDEDQKLRNESRKILGIPELAVSGTCVRVPVFTGHSLSINAEFARPLSVERARELL
                     ASAPGVKLVDVPTPLAAAGVDDSLVGRIRQDPGVPDGRGLALFVSNDNLRKGAALNTI
                     QIAELLAAEL"
     gene            complement(1836..3101)
                     /locus_tag="VF874_06475"
     CDS             complement(1836..3101)
                     /locus_tag="VF874_06475"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX9175745.1"
                     /translation="MALVVQKYGGSSVSDADRIRRVAERIVETKKKGDDVVVVVSAMG
                     DTTDDLLDLARQVCPSPPPREMDMLLTAGERISNALVAMAIESLGAQARSFTGSQAGV
                     ITTSIHGNAKIIDVTPGRLRSALDEGLIVLVAGFQGVSQDSKDVTTLGRGGSDTTAVA
                     LAAALHADVCEIYTDVDGIFTADPRIVSNARRLDKVSFEEMLELAACGAKVLMLRCVE
                     YARRYDVPIHVRSSYTDKPGTLVAGSMEDIPVEDAILTGVAHDRSEAKVTIVGIPDVP
                     GYAAKVFRAVADADVNIDMVLQNVSKIEDGKTDITFTCSRESGPRAVEELTSRQAEIG
                     FIKVLYDDHIGKVSLVGAGMRSHPGVTATFCEALARVGVNIDLISTSEIRISVLVKDT
                     ELDKAVAALHEAFGLGGDEVATVYAGTGR"
     gene            3183..3773
                     /locus_tag="VF874_06480"
     CDS             3183..3773
                     /locus_tag="VF874_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9175746.1"
                     /translation="MTDRRADTSVPINPVLAARWSPRAFDASAVLSPEQITALLEAAR
                     WAATWGDRQPVRFVVGIRGDETFTEVGDLLKRGNSYAKLASALILACIDEGPDEKTAR
                     YAAVDAGAAIAQLTVEAFSRGLVAHPMAGFNADGARAAFGLPDEVCPIAVVAVGALGD
                     YANVDPAVVERDARGRQRLPLAEIAFAGRWGRPFQP"
     gene            complement(3764..4663)
                     /locus_tag="VF874_06485"
     CDS             complement(3764..4663)
                     /locus_tag="VF874_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175747.1"
                     /translation="MLRPASSLAVALVAATLLTSCSAPIEGTPVIGPKDVDQSFFFAG
                     DVPVYGQQLSADDISYLAYLRALRRVDVCGLISTSANIGELVSVGTLFAFDECDAELK
                     VSGVSERRFVSVELEMSATAPSGCQALVPLPLSGLPAAPPLPEWMQPSVRVEPIGAQD
                     CELTRRIAGALAQRLASKPLPPRDAAATYPARLAERDPCEVLSVVKNVTRWDVPGSGP
                     YQCKFSTVDVGIEVGLQPQLIDAGPAPCSAVSFVGAPMQRRVIGVGYVDPTDVVIRPA
                     VVVEADDCQMAADVATKAAKLYG"
     gene            4797..6596
                     /gene="leuA"
                     /locus_tag="VF874_06490"
     CDS             4797..6596
                     /gene="leuA"
                     /locus_tag="VF874_06490"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9175748.1"
                     /translation="MTEEFADAFVSARTVTTPSGPPRAGQPAWNTQRGTAMPVHRYRP
                     FADEVEPIRLPDRTWPDRVITHAPTWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMG
                     YKEIEVGFPSASRTDFDFVREIIEQGAIPDDVTIQVLTQCRSELIERTFEACVGAPRA
                     IVHFYNSTSILQRRVVFRADRDEVRKIATDGARKCVEEAAKYPGTQWRFEYSPESYTG
                     TELEYAVEVCNAVGDVIQPTPENPIIFNLPATVEMATPNVYADSIEWMHRNLKRRDSV
                     ILSLHPHNDRGTAVAAAELGFQAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGIDP
                     QIDFSNIDEIRRTVEYCNQLPVPERHPYGGDLVYTAFSGSHQDAINKGLDAMKFHADD
                     VGSDVDDLLWQVPYLPIDPKDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLMLPRRLQ
                     IEFSQVIQKITDGEGGEVSSKEMWDVFADEYLTPVRPLERVRQRVVAAETDGGTDSIE
                     ATVRIDGAETEIRGEGNGPLAAFVHALSTVGVRVQVLDYSEHAMSSGEQAQAAAYVEA
                     SINGRTVWGVGIATSITTASLRAVVSAVNRAAR"
     gene            complement(6612..7604)
                     /locus_tag="VF874_06495"
     CDS             complement(6612..7604)
                     /locus_tag="VF874_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDDh family exonuclease"
                     /protein_id="HEX9175749.1"
                     /translation="MAETPWGRPVAEHDAGWVVLDVETSGFRPGTARIISLAALALNA
                     DGTVERSVASLLNPGVDPGPTHVHGLTADMLADQPSFAQIAGELIDMLGGRTLVAHNA
                     AFDYAFLAAEAELAEIELPVDTVMCTVELTRRLSLSTENLRLETLAAHWGVPQLRPHN
                     ALDDALVLSRVLTPAVERARELDVWLPVRPVSRRRWPNGRVTHDELRPLKMVAARVPC
                     RWLNPGRHVPGRRLVQGMRVVLSVDVRRTHEELIERIMRAGLTYTDMLDADTSFVICN
                     DPNAQQGKGFQARELGVPTISDAEFMAVLDSVIGGASIEEFTDPTVAGDQFALF"
     gene            7713..8945
                     /locus_tag="VF874_06500"
     CDS             7713..8945
                     /locus_tag="VF874_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX9175750.1"
                     /translation="MITARGRIALAAGATARWASRVTGRGAGSMIGGLVAMTLDRSLL
                     RQLGSGRRTVLVTGTNGKSTTTRMVASALGSLGPVATNSEGANMDAGLIAALAVARDA
                     GLAALEVDEMHLPHVADAVEPRVIVLLNLSRDQLDRVGEINAIERTLRAGLARHPDAV
                     VVANCDDVLMTSAAYDCPHVVWVAAGGGWANDSVSCPRSGEVIVRDKGHWYSTGTDFK
                     RPTPQWWFDDEMLYGPDGLALPMKLSLPGAVNRGNAAQAVAAAVSLGADPAAAVAAAS
                     GVSEVAGRYRTVTVGSHTARILLAKNPAGWQEALSMIDKHGAGVVIAVNGQVPDGEDL
                     SWLWDVRFQHFEGTSVVAAGERGTDLAVRLTYAGVEHTLVHDTIAAIESCPAGHVEVI
                     ANYTAFLQLQRALGRQRG"
     gene            8950..9645
                     /locus_tag="VF874_06505"
     CDS             8950..9645
                     /locus_tag="VF874_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX9175751.1"
                     /translation="MVRIGLVLPDVMGTYGDGGNAVVLRQRLLLRGIPAEIVEITLGD
                     PVPAELDIYTLGGAEDYAQRLATKHLLRYPGLQQASSRGAPVLAICAAIQVLGHWYET
                     SVGERIDGVGLLDLVTAPQPVRTIGEVVSRPLVDGLTEPFTGFENHRGGTVLGSSARP
                     LGAVAKGAGNRAGDGFDGAVQGSVVATYMHGPCLARNPQLADLLLSRVVGDLPALELN
                     EIALLRRERLAAR"
     gene            complement(9724..10335)
                     /gene="recR"
                     /locus_tag="VF874_06510"
     CDS             complement(9724..10335)
                     /gene="recR"
                     /locus_tag="VF874_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX9175752.1"
                     /translation="MFEGPVQDLIDELGKLPGIGPKSAQRIAFYLLSVEPPDIDRLTA
                     VLAKVRDGVRFCAVCGNVSDGERCRICSDPRRDASVVCVVEEPKDIQAVERTREFRGR
                     YHVLGGALDPLSGVGPDQLRIRELLNRIGEQVDGVEVAEVIIATDPNTEGEATATYLV
                     RMLRDIPGLSVTRIASGLPMGGDLEFADELTLGRALSGRRAMA"
     gene            complement(10347..10661)
                     /locus_tag="VF874_06515"
     CDS             complement(10347..10661)
                     /locus_tag="VF874_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX9175753.1"
                     /translation="MQPGDTPDMSALLAQAQQMQQQLMEAQEQLAAAEVHGQAGGGLV
                     QVTMKGSGEVVGVSIDPKVVDPSDVETLQDLIVGAFADASHQVTALAQSRLGPLAGGL
                     GI"
     gene            10798..11514
                     /locus_tag="VF874_06520"
     CDS             10798..11514
                     /locus_tag="VF874_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3717 family N-acetylmuramoyl-L-alanine
                     amidase"
                     /protein_id="HEX9175754.1"
                     /translation="MLVTASLVSAPHAAGQPANIAGMIVFLDPGHNGANDASITRQVP
                     NGRGGTKDCQASGTAADDGYPEHSFTWEVTLRIRSMLNQLGVRTAMSRGNDDALGPCV
                     DERAKMANDLHPNAIVSIHADGAPASGRGFHVNYSAPPLNQAQAGPSVQFAQVMRDQL
                     QASGIPPSNYIGNDGLYGRADLAGLNLAQYPAVLVELGNMKNPADEALITTVEGRQKY
                     AAAVVNGIAGFLAQNGGTAT"
     gene            complement(11511..11960)
                     /locus_tag="VF874_06525"
     CDS             complement(11511..11960)
                     /locus_tag="VF874_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9175755.1"
                     /translation="MGQVSASSSVLVNAAPEAVLAAVADYQTVRPKIVSSHYSDYQVL
                     QGGQGQGTVATWKLQATKSRVRDVQAEVDVAGHTVIEKDANSSMVTNWTVAPAGTGST
                     VTVKTSWQGAGGVKGFFEKTFAPLGLKRIQDEVLANLKKEVEPATSN"
     gene            12029..13384
                     /locus_tag="VF874_06530"
     CDS             12029..13384
                     /locus_tag="VF874_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9175756.1"
                     /translation="MSTHEDGVRRLIASYRAIPETATVRLAKPTSNLFRARAKRDAPG
                     LDTSGLTGVIAVDPEARTADVAGMCTYEDLVAATLPYGLAPLVVPQLKTITLGGAVTG
                     LGIESTSFRNGLPHESVLEMDILTGTGEVVTASPDQHQDLYRGFPNSYGTLGYSVRLK
                     IELEPVKPFVALRHLRFNSVSDLVAEMDRIADTGGYDGVPVEYLDGVVFSADESYLCI
                     GVQTGTAGPVSDYTGQQIYYRSIQHAAGEKHDRLTIHDYLWRWDTDWFWCSRAFGAQN
                     PRIRRWWPRRLRRSSFYWKLIAYDRRFNVADRIEKRSGRPPRERVVQDVEVPIERCGE
                     FLEWFLANVPIEPIWLCPLRLRDSDRWPLYPIRPGHTYVNVGFWSSVPVGATEGETNR
                     RIEAKVSELDGHKSLYSDAYYSVDEFDDLYGGETYKTLKKTYDPDSRFLDLYAKAVQR
                     R"
     gene            13381..14694
                     /locus_tag="VF874_06535"
     CDS             13381..14694
                     /locus_tag="VF874_06535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175757.1"
                     /translation="MTTFKERTHTLSGKLTLAQILEIFAEGKSPLRFTAYDGSTAGPD
                     DAELGVDLRTPRGTTYLATAPGELGMARAYVSGDVELHGVHPGDPYELLKALADKLEF
                     KRPPARVMANILRSIGFEHLKPIAPPPQEALPRWRRIAEGLRHSKTRDAEAIHHHYDV
                     SNRFYEWVLGSSMTYTCACYQHSGATLEEAQENKYRLVFEKLHLQPGDRLLDVGCGWG
                     GMVRYAARQGVETIGVTLSAKQAEWAQKAIADEGLTDLAEVRHGDYRDIGETQFDAVS
                     SIGLTEHIGVANYPAYFRFLQSKLRTGGLLLNHCITRHDNRHSATAGGFIDRYVFPDG
                     ELTGSGRIITEMQNVGLEVLHEENLRHHYAMTLRDWCRNLVEHWDEAVEEVGLPTAKV
                     WGLYMAGSRVGFEHNAIQLHQVLAVKLDERGDGDGGLPLRPWWTP"
     gene            complement(14578..15264)
                     /locus_tag="VF874_06540"
     CDS             complement(14578..15264)
                     /locus_tag="VF874_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9175758.1"
                     /translation="MSSVIPQVSDTQTRQRLIDVAVELFSRHSYAGTSLQMIADELGF
                     TKGAIYHHFRTREELLRAIVEPMLEQLRAIVEAAEQQRTAHARAEQMLRGYAAHLAAN
                     RKLAAVFALDPGVLEVLRTNPDWNRLIGRQMALLAGVESGPVGRVKATMVMAGIAAAT
                     HPDVADVDEDAVHSAVGSRPSHVGPAYAAPLGRPPRPQRQAAVAVAALVELDRQHLMQ
                     LNRVVLEANS"
BASE COUNT         2564 a         5256 c         5161 g         2528 t
ORIGIN      
        1 gtcgccgcac cgagcgcgcg gacggtgcgt tcgcgcgcgt acccccattt ggccagcgca
       61 ccgccgcagc gcgggcagca catgacggcg tcggcgagcc gggcctcggc gtcctcgggt
      121 cggcggacga cgatcactgc ccgacactgc gttcacggtg catcagtgtt gcctaccaca
      181 ccccggattg gggaaggggt cagttcgtgt tgggcgcaac agctttgggg tccgtgccgt
      241 cactcacttt gccgacgacg cccactcgtc acgatgcccc gcgtcgttcg gtacggcgcg
      301 ggcccgtcac cggctgcacc accgtacatc gcaacattgg tggtgccgac gaaagcccgc
      361 aacccgaacc ttcggcggca tccgaagtga cggagagacc ctccgtggtc gtcatacaga
      421 aggacgctca acagacgcgg tgatcgccaa aggcatggcc aaagaccccg acgagcgtta
      481 tcaaacaacg ctggaattgg ccaagtccgc gcgcgccgcg gtcaccaccc cgattccgca
      541 accgacagtg cccgctgcgg cgcagccgcc cacccagacg gggccaagtc gattcgagcc
      601 ggacggttca ccagcaatcg gcccagatgc cttcaccgcg gtgggcgggc tgcggattac
      661 gcgttgcccc ttccgccgca ggacccgatc atggtgctga ccgggcgtgg ccacttggag
      721 gtaatcggta ctgcctgcgc cgtcggcgtc gacttcgacg aaaagtttga gcgcacaggc
      781 gactaatgac taagccaaag ccctttacag ctcagcggcc agcagctcag cgatctggat
      841 ggtgttcagc gcggcgccct tgcgcaggtt gtcgttcgag acgaacagcg ccagcccgcg
      901 gccgtccggg acgccggggt cctgacggat gcgcccgacc agtgagtcgt cgacgcccgc
      961 cgcggccaac ggagtgggca catcgacgag ctttaccccc ggcgcactag ccagcagttc
     1021 gcgggcccgc tcaaccgaca gcggtcgggc gaactcggcg ttgatcgaca acgagtgccc
     1081 ggtgaacacc ggcacccgca cgcaggttcc gctgaccgcg agctccggaa tcccgaggat
     1141 cttgcggctc tcgttgcgca gcttctggtc ttcgtcggtc tcgccggagc cgtcgtcgac
     1201 cagcgacccg gccaacggca ccacgttgaa agcgattgga gcgacgtact tttcgggcgg
     1261 cgggaagtcc aacgcggccc cgtcgtgcac cagttgttcg gcgtcgtcga tcaccgcgcg
     1321 cgcttgcgcc gccaactcgt ccaccccggc gaccccactg cccgacaccg cctgatacgt
     1381 cgaggcgacc atccgcacca gctgcgcctc gtcgtgcagc accttgagca ccggcatcgc
     1441 ggccatcgtg gtgcagttcg ggttggcgat gatgcccttc ggccggttcg ccgctgcggc
     1501 gaagttcacc tcggaaacca ccagcggcac gtcagggtcc ttgcgccacg ccgacgagtt
     1561 gtcgatcacc gtcaccccgg ccgccgcgaa ccgcggcgcc tgcacccgcg acatcgtcgc
     1621 gccggcggag aacagcgcga tgtcaagccc ggcggggtcg gcgatggcgg cgtcctcgac
     1681 ctcgatctcc tgcccccgaa acgccagctt gcgccccgcc gaccgcgaag acgcgaagaa
     1741 ccgcaccgac gtcgccggaa aatcgcgctc ctccaacaac gttcgcatca caaggccgac
     1801 ctgaccggtg gcgccgacga ctccgatcgc aaccatcagc ggcccgtccc cgcgtacacc
     1861 gtggcgacct cgtcgccgcc gagcccgaac gcctcgtgga gcgcggcgac ggccttgtcg
     1921 agctcagtgt ccttgaccag caccgagatt cggatctcgg acgtcgaaat gagatcgatg
     1981 ttgacaccca cccgggccag cgcctcgcag aacgtcgcgg tgacaccggg atggctgcgc
     2041 ataccggcgc cgacaagtga caccttgccg atgtggtcgt cgtaaagcac tttgataaat
     2101 ccgatttcgg cctgtcgtga tgtgagttcc tcgactgcgc gcggcccgct ctcgcgcgaa
     2161 caggtgaacg tgatgtcggt cttgccgtct tcgatcttgg atacgttctg caacaccatg
     2221 tcaatgttca cgtcggcgtc ggcgacagcg cgaaaaacct tggcggcgta gcccggaacg
     2281 tcggggatgc cgacgatcgt gaccttggcc tcgctgcggt cgtgtgcgac tccggtcaga
     2341 atggcgtctt ccacgggtat gtcctccatc gatccggcca ccaatgtgcc gggcttgtcg
     2401 gtgtacgacg accgcacgtg tatcggaacg tcgtagcggc gtgcgtattc cacgcagcgc
     2461 agcatcagca ccttcgcgcc gcacgcagcc aactcgagca tctcctcgaa gctgaccttg
     2521 tccagccggc gcgcgttgga cacgatcctc gggtcggcgg tgaagatccc gtcgacgtcg
     2581 gtgtagatct cgcacacgtc ggcgtgcagc gcggcggcca gcgcgaccgc ggtggtgtcc
     2641 gatccgccgc ggcccaacgt ggtgacgtcc ttgctgtcct ggctgacccc ctggaatccg
     2701 gcaacaagca cgatcagccc ctcgtcgagc gccgaacgca accggcccgg ggtgacgtcg
     2761 atgatcttcg cgttgccgtg gatgctcgtg gtgatcacgc cggcctgcga cccggtgaac
     2821 gagcgggcct gcgcgccgag cgactcgatg gccatcgcga ccagcgcgtt gctgatgcgt
     2881 tcacccgcgg tcagcagcat gtccatctcg cgcggcggcg gcgacgggca cacctgcctg
     2941 gcaaggtcca gcaggtcgtc ggtggtgtcc cccatcgccg acaccacgac gacgacgtcg
     3001 tcgcccttct tcttggtttc gacgatgcgc tcggcgacgc gacggatgcg gtcggcgtca
     3061 gacaccgacg atccgccgta tttctgcacg acgagcgcca cgaaggaacc tttctggagc
     3121 gggtttctcc aagaataagg gctgcgcgca tttcattttt gccgtcggta gcgtggggaa
     3181 ccgtgaccga tcggcgcgcc gacaccagcg tcccgatcaa tccggtgctc gccgcccgct
     3241 ggagccctcg cgcgttcgac gcgtccgccg tgctgagccc cgagcagatc accgcgctgc
     3301 tcgaggctgc ccgctgggcc gcgacgtggg gcgatcgcca gccggtgcgg ttcgtcgtcg
     3361 gtatccgcgg cgacgaaaca ttcactgaag tgggcgacct gctcaagcgg gggaacagtt
     3421 acgcgaagct ggccagcgcg ttgatcctgg cctgcatcga cgagggcccg gacgagaaga
     3481 ccgcgcggta cgcggcggtc gacgccggcg ctgcgatcgc acagctgacg gtcgaggcgt
     3541 tttcgcgtgg gttggtcgcc cacccgatgg cggggttcaa cgccgacggc gcgcgcgccg
     3601 cgttcggcct gcccgacgag gtctgtccga tcgcggtcgt cgcggtcgga gcgctcggcg
     3661 actacgccaa cgtcgatccg gcggtcgtcg agcgcgacgc gcgcggacgg caacgcctgc
     3721 cgctggcgga gatcgcgttc gcgggccgct ggggacggcc gtttcagccg tagagtttcg
     3781 cggccttcgt cgcgacgtca gctgccatct gacagtcgtc cgcctcgacg acgacggcgg
     3841 gccggatcac cacgtcggtg gggtcgacgt atccgacgcc gatcaccctg cgctgcatcg
     3901 gtgcgccgac gaacgaaacc gccgagcacg gcgcagggcc ggcgtcgatg agttgcggct
     3961 gcaaccccac ctctataccg acgtcaacgg tgctgaactt gcattggtac ggccccgagc
     4021 caggcacgtc ccaccgcgtg acgtttttca cgactgacag cacctcgcac gggtcgcgtt
     4081 cggcgagccg agccgggtag gtggccgccg cgtcgcgtgg cggcagcggc ttgctggcca
     4141 gccgttgagc aagcgcgccg gcgatccgcc tggtcagctc gcagtcctgc gcgccgatcg
     4201 gctcgacccg aaccgacggc tgcatccact caggcagcgg cggagccgcc ggcaacccgc
     4261 tcagtggcag cggtaccaat gcctggcagc cgctgggcgc agttgccgac atctccaact
     4321 ccaccgacac gaaacgccgc tcgctgactc cgctgacctt gagttcggcg tcgcattcgt
     4381 cgaacgcgaa aagtgttccg actgagacca attcaccgat gttggctgac gtactgatca
     4441 gcccgcacac gtcgacgcgg cgcagcgcgc gcaggtaggc caggtagctg atgtcatcgg
     4501 cgctgagctg ctgaccgtag acgggaacgt cgccggcgaa gaagaagctc tgatcgacgt
     4561 ccttgggccc gatgacgggc gtgccctcga tcggggctga gcagctcgtc agcagcgtcg
     4621 cggcgaccag tgccacagcc aatgaactgg ccggtcgcag catgcggtac agtaaccgac
     4681 gtgcagcgga tgctccttct cggcagccgc gacggggtct gatcagaccg gcttcccgtc
     4741 gcgggtagtc gcgatgtgcc ggtcgtagtt caataaactc ccggaaagca gccccaatga
     4801 ctgaagaatt cgccgacgcg ttcgtatcgg cacgcactgt caccactccg tccggacccc
     4861 cacgcgccgg gcagcccgcg tggaacaccc agcgcggcac ggcgatgccg gtgcatcggt
     4921 atcgtccgtt cgccgacgag gtcgagccga tccgcctgcc cgaccgcacc tggccggatc
     4981 gcgtgatcac gcacgccccg acgtggtgtg cggtggacct gcgcgacggc aaccaggcgc
     5041 tgatcgaccc gatgagcccg gcccgcaaac gccggatgtt cgatctgctc gtccggatgg
     5101 gctacaagga gatcgaggtc ggcttcccgt cggccagccg gaccgacttc gacttcgtcc
     5161 gcgaaatcat cgagcagggt gcgattcccg acgatgtcac gatccaggtg ctgacgcagt
     5221 gccgctcgga gctgatcgag cggacgttcg aggcctgtgt gggcgccccg cgcgcgatcg
     5281 tgcatttcta caactcgacg tcgattctgc agcgccgcgt ggtgttccgc gccgaccgcg
     5341 acgaggtccg caagatcgcc accgacggcg cccgcaagtg cgtcgaagaa gccgcgaaat
     5401 accctggcac gcaatggcgg ttcgagtatt caccggagtc ctacaccggc accgaactcg
     5461 aatacgcggt cgaggtgtgt aacgccgtgg gcgacgtgat tcagccgacc ccggagaacc
     5521 cgatcatctt caacctgccc gccactgtcg agatggccac gcccaacgtc tacgccgact
     5581 cgatcgagtg gatgcaccgc aacctaaagc gccgcgacag cgtcatcttg agcttgcacc
     5641 cgcacaacga ccgcggaact gccgtcgccg cagccgaatt gggcttccaa gccggggccg
     5701 accggatcga gggctgcctg ttcggcaacg gcgagcgcac cggcaacgtc tgcctggtaa
     5761 cgctgggctt gaacctgttc agccgcggca tcgacccgca gatcgacttc agcaacatcg
     5821 acgagatccg ccgcacggtc gagtactgca accagctgcc cgtgcctgaa cgccatccgt
     5881 acggcggcga cctggtgtac accgcgttct cgggcagcca ccaggacgcg atcaacaagg
     5941 gtctggacgc gatgaagttt catgcggacg acgtcggctc cgatgttgat gacctcctgt
     6001 ggcaagtccc ttacctgccg atcgacccca aggacgtcgg ccgcacatat gaggccgtga
     6061 tccgggtgaa ctcgcagtcg ggcaagggcg gcgtcgccta catcatgaag gccgaccacg
     6121 ggctgatgct gccgcgccgg ttgcagatcg agttctctca ggtgatccag aagatcaccg
     6181 acggcgaggg cggcgaagtg tcgtcgaagg agatgtggga cgtcttcgcc gacgagtact
     6241 tgacgccggt tcgcccgctg gagcgcgtcc ggcagcgcgt cgtcgccgcc gaaaccgacg
     6301 gtggcactga ttcgatcgag gcgaccgtgc gcatcgacgg agcggagacc gagatccgcg
     6361 gcgagggcaa cggcccgctc gcggcgttcg tgcacgcgct gtcgaccgtc ggcgtccgcg
     6421 tgcaggtgct cgactattcc gagcacgcga tgtcctcggg cgagcaggcg caggccgcgg
     6481 cctacgtcga ggcgtcgatc aacggccgca cggtgtgggg cgtggggatc gcgacgtcga
     6541 tcaccactgc gtcgctgcgg gcggtggtgt cggcggtgaa ccgcgcggcg cgctaggtca
     6601 gacgcgctag gtcagaaaag tgcgaactgg tcgcccgcga cggtcgggtc ggtgaactcc
     6661 tcgatcgacg cgccgccgat cacagagtcc agcacggcca tgaactcggc gtccgagatc
     6721 gtcggcaccc ccaattcccg tgcctgaaaa cccttgccct gctgggcgtt cgggtcgttg
     6781 cagatcacaa acgacgtgtc cgcgtccagc atgtcggtgt aggtgagtcc ggcgcgcatg
     6841 atccgctcga tcagctcctc gtgagtgcgc cgtacgtcca cggacaacac gacgcgcatg
     6901 ccctgcacca gccgacgccc cggcacgtgc cggcccgggt tcagccaccg gcatggcact
     6961 cgcgcggcga ccatcttgag cggccgcagt tcgtcatgcg tcacgcggcc gttcggccag
     7021 cgccgccgcg acaccggccg caccggcagc cacacgtcga gctcacgcgc ccgctccacc
     7081 gcgggcgtca gcaccctcga caacacgagc gcgtcgtcga gcgcgttgtg cggacgtaac
     7141 tgcgggacgc cccagtgtgc ggccaaggtt tccagccgca ggttctcggt gctcagcgac
     7201 aggcgcctgg tcagctcgac ggtgcacatc acggtgtcga ctggcagctc gatctcggcc
     7261 agctcggctt cggcggccaa gaacgcgtag tcgaacgccg cgttgtgggc gaccagcgtg
     7321 cggccgccca gcatgtcgat cagctcgccg gcgatctgtg cgaacgacgg ctggtcggcg
     7381 agcatgtcgg cggtgagccc gtgcacgtgg gtggggcccg gatcgactcc cgggttcagc
     7441 aggctggcca ccgagcgttc gactgtgccg tcggcgttca gcgccagcgc ggcgaggctg
     7501 atgatccgcg cggtgcccgg ccggaagccg gaggtctcga cgtccagaac cacccagccg
     7561 gcgtcatgct cggcgaccgg cctgccccac ggggtctctg ccagcttcgg tggcgctgtc
     7621 atgtccccca gggtcgcacg gtgtaccgac aaaaccgctg atcgcagcgt tcgtgtcgca
     7681 ggtcagtagc cgtttcgtca taaactgccc cgatgatcac tgcccgcgga cgcatcgcgc
     7741 tcgcggccgg agcgaccgcg cggtgggcgt cgcgggtaac cggccgaggg gccggttcga
     7801 tgatcggcgg cctggtggcg atgacgctgg accgctcgct gctgcgccag ctcgggtccg
     7861 gtcggcgaac ggtgttggtg acgggcacca acggcaagtc gacgacgacg cggatggtcg
     7921 cctcggcgtt ggggtcgctc ggcccggtgg cgacgaactc cgagggcgcg aacatggacg
     7981 ccgggctgat cgccgcgctg gcggtggccc gggacgctgg tttggcggcg cttgaggtgg
     8041 acgagatgca tctgccgcac gtggctgacg cggtggagcc gcgggtgatc gtgctgctca
     8101 acctgtcgcg cgaccaactc gaccgcgtcg gcgagatcaa cgcgatcgaa cggacattgc
     8161 gtgccgggct ggcccgacat cccgatgcgg tggtggtggc caactgcgac gacgtattga
     8221 tgacgtcggc ggcctacgac tgcccgcacg tggtgtgggt ggcggccggc ggcggctggg
     8281 ccaacgactc ggtgagctgc ccgcggtccg gcgaggtgat cgtccgcgac aagggccact
     8341 ggtacagcac cggaaccgac ttcaagcggc cgacgccgca gtggtggttc gacgacgaaa
     8401 tgctttacgg cccagacggt ttggctctac cgatgaagct gtcgctgccg ggcgcggtga
     8461 accggggcaa cgccgcgcag gctgttgccg cggccgtgtc tttgggtgcc gaccccgctg
     8521 cggcggtggc ggcggcgtcg ggcgtgtccg aggtggcggg ccgctaccgc acggtgactg
     8581 tgggctcgca taccgcgcgg atcctgctgg ccaagaaccc ggcgggctgg caggaggcgc
     8641 tgtcgatgat cgacaagcac ggcgccggcg tcgtgatcgc ggtgaacggg caggtacccg
     8701 acggcgagga cctgtcgtgg ctatgggacg tgcggttcca acatttcgaa ggaacttcgg
     8761 tcgtcgccgc cggtgagcgc ggcaccgacc tggccgtgcg gctgacctac gccggggtgg
     8821 aacacacgct ggtgcacgac accatcgcgg ccatcgagtc gtgcccggcg ggacacgtcg
     8881 aggtgatcgc gaactacacc gcgttcctac agctccagcg cgcattgggg cgccagcgtg
     8941 gctgattcgg tggtgcggat cggcctggtg ctgcccgatg tgatgggcac ctacggcgac
     9001 ggcggcaatg cggtggtgct gcggcagcgt ctgctgctgc gagggattcc cgccgagatc
     9061 gtcgagatca ctttgggcga tcccgtgccc gccgagttgg acatctacac gttgggcggg
     9121 gccgaggact atgcgcagcg gctcgccacc aagcacctcc tgcgctatcc cgggctgcag
     9181 caggcgtcgt cacgtggcgc gccggtgttg gcgatctgcg cggcgatcca ggtgctcggc
     9241 cactggtatg agacgtcggt aggtgagcgc atcgacgggg tcggcctgtt ggatctggtg
     9301 acggcgccgc agccggtgcg caccatcggc gaagtggtga gccggccact ggtcgacggg
     9361 ttgacggagc cgtttacagg gttcgaaaac caccgcggcg gaacggtttt gggctcgtcg
     9421 gcgcggccac tcggcgcggt ggcgaagggg gcgggcaatc gggcgggcga cggcttcgac
     9481 ggcgcggtgc aaggcagcgt cgtcgccacc tacatgcacg gcccgtgcct ggcccgcaat
     9541 ccccagctgg ccgacctgct gctgtcgagg gtggtcggcg acttgccggc gttggagctc
     9601 aacgagatcg cgctgctgcg ccgcgaacgg ctcgcagcgc gctgactgcg tccagagcga
     9661 cgttttgcag aagaagtgtg agaagggggc ctgaaaaacg tcactctcga cgcatgcggg
     9721 cgctcaggcc atcgcgcggc ggcccgacag cgcccggccc agcgtgagct cgtcggcgaa
     9781 ctcgaggtcg ccgcccatcg gcaacccgga cgcgatgcgc gtcacgctca aacccgggat
     9841 gtcgcgcagc atccggacga gatacgtcgc ggtcgcctca ccttcggtgt tcgggtccgt
     9901 cgcgatgatc acctcagcga cctcgacacc gtcgacctgt tcaccgatgc ggttcaacag
     9961 ctcgcgaatt cgcaactggt ccggtccgac gccggacagc ggatccagcg ccccgcccag
    10021 cacgtgatag cgaccccgga actcccgcgt gcgctcgacg gcctgaatgt ccttcggctc
    10081 ctcgaccacg cacaccaccg acgcgtcgcg ccgcgggtct gagcagatgc ggcagcgctc
    10141 gccgtcggag acgttgccgc acaccgcaca aaaccgcacg ccgtcgcgca ccttggccaa
    10201 caccgcagtc agccggtcga tgtccggcgg ctcgaccgac agcaggtaaa acgcgatccg
    10261 ctgagcgctc ttcgggccga ttcccggcag cttgcccaac tcgtcgatca ggtcctggac
    10321 ggggccctca aacatgggag tacaagtcag atccccaacc cgccggccag cgggcccagt
    10381 cggctctgcg cgagggctgt cacctgatgc gaggcatccg caaacgcccc gacgatcagg
    10441 tcctgcaacg tctcgacatc ggaaggatcg accaccttgg gatcgatcga caccccgacc
    10501 acctctccgc tgcccttcat cgtgacctgc accagcccgc cgccggcctg gccgtgcacc
    10561 tcggcggcgg ccagctgttc ctgcgcttcc atcagttgct gctgcatctg ctgcgcctgc
    10621 gccagcagcg ctgacatgtc cggcgtgtct ccgggttgca tcacagtcct cttgcctgtt
    10681 ggtctcgagt tcgtttcagc tgggaatcga ctctgcccag ggtagtcgtg ggcaagctac
    10741 tttgacgcag tgccagagct gttgcgcgtg cgtgtcggtg ccgctgcggc cgccgcagtg
    10801 ctcgtcactg cgtcgctggt ttctgcgccg cacgcggctg gccagcctgc caacatcgcc
    10861 gggatgatcg tgttcctcga tcccggccac aacggcgcca acgacgcatc gatcacccgg
    10921 caggtgccca acgggcgagg cggcacgaag gactgccagg cctcgggcac cgccgccgac
    10981 gacggctacc ccgagcactc gttcacctgg gaagtcacgc tgcggatccg ctcgatgctg
    11041 aaccagctgg gcgtgcggac cgcgatgtct cgcggcaacg acgacgcgct tggcccgtgc
    11101 gtcgacgaac gcgccaagat ggccaacgat ttgcacccta acgcgatcgt gtccatccac
    11161 gccgacggcg cccccgcgtc gggccgcgga ttccacgtca actactccgc gccacccctc
    11221 aaccaagccc aagccggacc gtcggtgcag ttcgcccagg tcatgcgcga ccagctgcag
    11281 gcttccggga ttccgccctc gaactacatc ggcaacgacg ggctctacgg tcgcgcggat
    11341 ctcgccgggc tcaacctcgc gcaatacccg gcggtgctcg tcgagctcgg caacatgaag
    11401 aacccggccg acgaggcgct gatcaccacc gtggagggcc ggcagaagta cgccgccgcg
    11461 gtggtcaacg gcatcgccgg ctttctggcg cagaacgggg gaacggccac ctagttggac
    11521 gtcgccggct cgacttcctt cttcaggttg gccagcacct cgtcctgaat cctctttagg
    11581 cccagcggcg cgaacgtctt ctcgaagaac cccttgacgc ctccggcgcc ctgccagctc
    11641 gtcttgacgg tcaccgtcga tccggtgccc gcgggggcga ctgtccagtt ggtcaccatc
    11701 gacgagttgg cgtccttctc gatcacggtg tggcctgcga cgtcgacctc ggcctgcacg
    11761 tcgcgcaccc gtgacttggt ggcctgcagc ttccacgtcg cgacggtgcc ctgaccttgg
    11821 ccgccctgca gcacctggta gtcgctgtag tgcgacgaca cgatcttcgg gcggacggtc
    11881 tggtagtcgg caaccgcggc cagcaccgcc tcgggtgcgg cgttgaccag aacgctgctg
    11941 gacgcgctga cctgtcccat caggcaagcc tcctctggtg gtgggcgggt cggtcgcatc
    12001 ggctgcgacc gcggactagc gtatatgcgt gtccacgcac gaagatggcg tccgccggct
    12061 aatcgccagt taccgtgcga tccccgagac cgccactgtc cggctggcca agccgacgtc
    12121 caacttgttc cgcgcgcggg ccaagcgcga cgcacctggc ctggacacgt cggggctgac
    12181 gggcgtgatt gcggtcgacc cggaggcgcg caccgccgat gtcgccggca tgtgcaccta
    12241 tgaggacctg gtggccgcga cgctaccgta cgggttggcg ccgctggtgg tgccgcagct
    12301 gaagacgatc acattgggcg gtgcggtcac cgggctcggg atcgagtcga cgtcgttccg
    12361 caacgggctg ccgcacgagt cggtgctcga gatggacatc ctcaccggca ccggcgaggt
    12421 ggtcaccgca tcaccggacc agcaccaaga cctgtatcgg ggtttcccga attcctatgg
    12481 cacacttggc tattcggtgc gcctgaagat cgagctggaa cccgtcaagc cgttcgtcgc
    12541 gttgcggcac ttgaggttta actcggtttc agatttggtg gccgaaatgg accgcatcgc
    12601 cgacaccggc gggtacgacg gcgttccggt cgaatacctc gacggcgtcg tgttcagcgc
    12661 cgacgaaagc tatctgtgca tcggggtgca gaccggcacg gccggaccgg tcagcgacta
    12721 caccggccaa cagatctact accgctcgat ccagcatgcc gccggcgaga agcacgaccg
    12781 gctgaccatc cacgactacc tgtggcgctg ggacaccgac tggttctggt gctcgcgggc
    12841 gttcggcgcg cagaacccgc gcatcaggcg gtggtggccg cggcggctgc ggcgcagcag
    12901 cttctactgg aagctcatcg cctacgaccg gcggttcaac gtcgccgacc ggatcgagaa
    12961 gcgcagtggc cgaccgccac gcgagcgggt ggtgcaggac gtcgaggtgc cgatcgaacg
    13021 ctgcggcgag ttcctcgagt ggttcctggc caacgttccc attgagccga tctggctgtg
    13081 cccgttgcgt cttcgcgatt ccgacaggtg gccgctgtac ccgatccggc ctgggcacac
    13141 gtacgtcaac gtggggttct ggtcgtcggt gccggtcggt gccaccgagg gcgagacgaa
    13201 ccggcggatc gaagcgaagg tcagcgagct cgacggacac aagtcgctgt actccgacgc
    13261 ctactactcc gtcgacgagt ttgacgatct ttacggcggt gagacgtaca agacgctaaa
    13321 gaagacgtac gatcccgact cacgcttcct tgacctctac gcgaaggcgg tgcaacgacg
    13381 atgaccacgt tcaaggaacg cacgcacacc ctgtcaggca agctcacctt ggcgcagatc
    13441 ctcgagatct tcgccgaggg gaagtcgccg ctgcgattca ccgcgtacga cggcagtacc
    13501 gctgggccgg acgacgcgga actaggcgtg gacctgcgta ctccgcgcgg gacgacgtac
    13561 cttgccaccg cgccgggcga actcggaatg gctcgggcat atgtgtccgg cgacgtcgaa
    13621 ctgcatggcg ttcatcccgg cgatccctac gagctcttga aagcattggc cgacaagctc
    13681 gaattcaagc ggccgccggc gcgtgtgatg gccaacatcc tgcgctcgat cggcttcgag
    13741 cacctcaagc ccatcgcgcc gccgccgcag gaggcgttgc cgcggtggcg tcggatcgcg
    13801 gaagggttgc ggcacagcaa aactcgtgac gccgaagcca tccaccacca ctacgacgtg
    13861 tcgaacaggt tctacgagtg ggtgcttggt tcgtcgatga cgtacacctg cgcgtgctat
    13921 cagcacagcg gcgcgactct ggaggaggcg caggagaaca agtaccggct ggtgttcgaa
    13981 aagctgcatc tgcagcccgg cgaccggctg ctcgacgtcg gatgcggttg gggcggcatg
    14041 gtgcgctacg cggcgcgcca gggcgtcgag acgatcggcg tgacgttgag cgccaaacaa
    14101 gcggagtggg cgcagaaggc gatcgccgac gaggggctga ccgacctcgc cgaggtccgg
    14161 cacggcgact accgcgatat cggtgagacg cagttcgacg cggtttcctc gatcggtctc
    14221 accgaacaca tcggcgtcgc gaactacccg gcgtatttcc gcttcctgca atcgaagctg
    14281 cgcaccggag ggctgctgct caaccactgc atcacccgtc acgacaaccg gcacagcgca
    14341 accgcgggcg ggttcatcga ccgctatgtg ttccccgacg gggaactcac cggttccggg
    14401 cggatcatca ccgaaatgca gaacgtcggt ctcgaggtgc tgcacgagga gaacctgcgc
    14461 caccactacg cgatgacact tcgtgactgg tgccggaacc tggtcgagca ctgggacgag
    14521 gccgtcgaag aggtggggtt gcccaccgcg aaggtgtggg gcctgtacat ggccggttca
    14581 cgagttggct tcgagcacaa cgcgattcag ttgcatcagg tgctggcggt caagctcgac
    14641 gagcgcggcg acggcgacgg cggcctgccg ctgcggccgt ggtggacgcc ctagcggcgc
    14701 ggcgtacgca ggcccaacgt gcgacggccg gcttccaaca gcagagtgca cagcgtcttc
    14761 gtcgacgtcc gcgacgtcgg ggtgggtggc cgccgcgatg ccggccatga ccatcgtcgc
    14821 cttgactcgg ccgaccggcc cggattcgac acctgccagc agcgccattt gcctgccgat
    14881 tagacggttc cagtccgggt tggtgcggag cacctccagc acccccgggt cgagggcgaa
    14941 caccgcagcc agtttgcggt tggctgccag gtgggcggcg tagccgcgga gcatctgttc
    15001 ggcacgcgcg tgcgcagtgc gctgctgctc ggccgcttcg acgatcgccc gcagctgttc
    15061 gagcatcggt tcgacgatcg cccgcagcag ttcctcacgc gtgcgaaaat ggtggtagat
    15121 cgcgcccttg gtgaagccga gctcgtcggc aatcatctgc agtgacgtgc ccgcatagct
    15181 gtgcctgctg aacagttcga ccgcgacgtc gatcagacgc tggcgcgtct gagtgtcgct
    15241 gacctgcggt atcacgctgc tcatggttct cctccggccg agtgccctat ctcaccctag
    15301 ctggtcgatc gtccagttga taactggacg atcgtatagt aacttccaag acgatcgtcc
    15361 agccaaggag cacacgcatc atgaccgcag caccggattt ctccgcgacg acgacctcgt
    15421 ggacgatccc tatccctact tcgaggccat gcgcaaccgg tgcccggtcg cccgtgaaga
    15481 ccaccacggc gtgaccatgg tgaccggct
//
LOCUS       DASWBF010000184         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1204998,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000184 DASWBF010000000
VERSION     DASWBF010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204998"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VF874_06545"
     CDS             complement(<1..741)
                     /locus_tag="VF874_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9175759.1"
                     /translation="MITRLIRIQLIVFVVLTVIALVVLGWYYLRVPRLMGVGQYTLYA
                     ELPRSGGLYPTANVTYRGITIGRVTDVEPTERGVRATMSIDSRYKIPENASANVHSVS
                     AVGEQYVDLVSTGNVNQYLRDGQTIATGTVPSEIGPALDAANRGLAALPADKIDGLLT
                     EASQAVGGLGPALQRLVDSTTAIASDFRDNLAPVNDIIAHSAPILDSQVNSGDAIGAW
                     SANLNVIGAQAAEQDAALRSGLQQAAPTA"
     gene            complement(745..1899)
                     /locus_tag="VF874_06550"
     CDS             complement(745..1899)
                     /locus_tag="VF874_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HEX9175760.1"
                     /translation="MKRTASIGRLARRGVALLAIALMVSSCGSWRGIGNVPLPGGRGS
                     GPDHMTIYVQMPNTLALNVNSRVRVADVYVGAVKAIELKNWVATLTLELQPSVKLPAN
                     ATAKIGQTSLLGSQHIELDVPPNPSPQPLKDGDTIPLKNASAFPTTERVLASFSMILR
                     GGGIDDLEVIQTEISNILMGRAPEIRGFLNKLDTFTDELNQQRDDLTRAIDQSNRLLA
                     IVAENNETLDRVFTEFTPLAKHFADTRDLFADAVQAIGRISRAADDALAPASDNLHTN
                     LALLQRPLRELGKASPYLIGALKIMLTAPYNIENVPKAIRGDYINVSILADLTLSAID
                     NGLLSGTGVSGMLRALEQSWGRDPATMIPDVRFTPNPHDAPNGPLVERHE"
     gene            complement(1896..>3267)
                     /locus_tag="VF874_06555"
     CDS             complement(1896..>3267)
                     /locus_tag="VF874_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9175761.1"
                     /translation="MKITFHYSNKYKVPADATASILNPTLVASRTIQLSPAYTGGPVL
                     KDGAVIPIERTQVPVEWDELRNEIDNLLSGLGPTPDQPKGPFGDLIESAANGLAGKGQ
                     QFNKTLNSLSEALTALNKGRGDFFGVVRSLALFVNALYQNDQQFVALNNDLASFTNKF
                     TNTDREVSNALRDINQLLTTTRSFLDENSEVLVHDIDNLADVTTTILQPEPRDGLETG
                     LHVFPNLAANIVNMTSPIAGGIVGLPVIANFANPMQFVCSAIQAGSRLGYQESAELCA
                     QYLAPILDAIKFNYLPFGLNQISSAATLPKVVAYSEERLRPPPGYKDTTVPGIFSRDT
                     LFSHGNHEPGWVVAPGMQGVDVQPFTANMLTPESLAELMGGPDIVAPPAPPAFGVPPG
                     GNLPGPPDAYSENTPLPPPWYPQPAPPPPPAPGVSQVPMVAPAPVGAPAAPAPPGGFP
                     AEGMGQ"
BASE COUNT          523 a         1056 c         1099 g          589 t
ORIGIN      
        1 ggccgttggg gccgcctgct gcaacccgct gcgcagcgcc gcgtcctgct cggcggcctg
       61 cgccccgatc acgttcaggt tggccgacca cgccccgatc gcgtccccgg agttgacctg
      121 cgaatccagg atcggcgccg agtgggcgat gatgtcgttg accggggcca ggttgtcgcg
      181 gaaatcgctg gcgatcgccg tggtggaatc caccagccgc tgcagcgccg ggcccagccc
      241 gccaaccgcc tgcgacgcct cggtgagcag cccgtcgatc ttgtcggcgg gcaacgccgc
      301 caggccccga ttggcggcat ccaacgccgg cccgatttca gaaggcaccg tgcccgtggc
      361 gatggtctgc ccgtcgcgga gatactggtt gacgttgccg gtggacacca ggtcgacgta
      421 ctgctcgccg acggccgaca ccgagtgcac gttcgccgac gcgttttcgg ggatcttgta
      481 ccggctgtca atgctcatcg tggcccgcac gccccgctcg gtcggctcga catcggtgac
      541 cctgccgatc gtgataccgc ggtacgtcac gttcgctgtc ggatacaacc ccccggatct
      601 cggcagctcc gcatacaacg tgtactggcc aacgcccatc aaccgcggca cccgcaggta
      661 gtaccaaccg agcaccacca gtgcaatcac cgtcaacacc acgaagacga tgagctggat
      721 gcggatgagg cgagtgatca cgcctcactc atgcctttcc accagtggcc cgttcggggc
      781 gtcgtgcggg ttcggggtga accggacgtc cgggatcatc gtggcgggat cacggcccca
      841 cgactgctcg agcgcgcgca gcatgccgga gacaccggtg ccggacaaca ggccgttgtc
      901 gattgcactc agggtcaggt cggcgagtat cgacacgttg atgtagtcgc cccgaatggc
      961 tttcggcacg ttctcgatgt tgtagggcgc agtgagcatg atcttcaacg caccgatcag
     1021 gtacggcgat gccttgccca gttcacgcag cggtcgttgc agcagagcca ggttggtgtg
     1081 gaggttgtcg ctcgcgggag ccaacgcgtc gtcggccgcc ctgctgatcc ggccgatcgc
     1141 ctgcacggcg tcggcgaaca ggtcgcgggt gtcggcgaaa tgctttgcca gcggcgtgaa
     1201 ttcggtgaac acccggtcca atgtctcgtt gttctcggcg acgatcgcca gcagccggtt
     1261 cgactggtcg atcgcgcgcg tcagatcatc acgttgctgg ttcagctcgt cggtgaacgt
     1321 gtccaacttg ttcaagaacc cccggatctc cggtgcgcgg cccatcagga tgttgctgat
     1381 ctcggtctgg atgacctcca ggtcatcgat tccgccgccc cgcaagatca tcgagaagga
     1441 cgccaacacc cgctcggtcg tcggaaaagc cgatgcgttc ttcaatggga tcgtgtcgcc
     1501 gtccttcaac ggctgcggcg acgggttcgg cggcacgtcc agctcgatat gctgcgaccc
     1561 caacaagctg gtctgcccga tcttggccgt cgcgttggcg ggcagcttca cgctcggctg
     1621 caattccagg gtcaacgtcg cgacccagtt cttgagctcg atcgccttga ccgcgccgac
     1681 gtacacgtcg gcgacccgca cgcggctgtt gacgttgagc gccaacgtgt tcggcatctg
     1741 gacgtagatg gtcatgtggt ctggaccgga gccccgccca cccggcaacg gcacgttccc
     1801 gatgccgcgc cacgaaccgc acgacgacac catcagggcg atcgccagca gggcgacgcc
     1861 gcgtcgcgct aaccgtccga tactcgcggt ccgcttcact ggcccatccc ctcagccggg
     1921 aagccgccgg gcggtgcagg cgcggccggt gcacccaccg gcgccggcgc gaccatcggg
     1981 acttgggaaa cacctggcgc cggaggcggc ggcggcgcgg gctgcggata ccacggcggc
     2041 ggcagcgggg tgttctcgct gtacgcgtcc ggcggcccgg gcaggtttcc gccgggcggc
     2101 accccaaacg cgggcggcgc cggcggcgcg acgatgtcgg gtccgcccat caactcggcg
     2161 agcgactcgg gggtgagcat gttcgcggtg aacggctgca cgtccacacc ctgcatgccc
     2221 ggggcgacga cccagcccgg ctcgtggttg ccgtgcgaga acaacgtgtc gcgggagaag
     2281 atgcccggca cggtggtgtc cttgtagccc ggcggcggac gcagtcgttc ctcggagtag
     2341 gcgaccacct ttggcaacgt ggcggctgag ctgatctggt tcaacccgaa cggtaggtag
     2401 ttgaacttga tcgcgtcgag aatcggagcc agatactgtg cgcacaactc cgcggactcc
     2461 tgatagccca gccggctgcc ggcctggatc gcgctgcaga cgaattgcat cgggttcgcg
     2521 aagttggcga tcacgggcag gccgacgatg ccgccggcga tcggcgaggt catgttgacg
     2581 atgttggccg cgaggttcgg gaacacgtgc aggccggtct ccaagccgtc gcgtggctcc
     2641 ggctgcaaga tcgtggtcgt gacgtcagcc aggttgtcga tgtcgtggac cagcacctcg
     2701 ctgttctcgt cgaggaaaga gcgggttgtg gtcagcagct ggttgatgtc gcgcagcgcg
     2761 ttggacactt cacggtcggt gttggtgaac ttgttcgtga agcttgcgag gtcgttgttc
     2821 aacgcgacga actgctggtc gttctggtac agcgcattga cgaacagcgc caggctgcgc
     2881 accacgccga agaagtcgcc gcggcccttg ttgagcgcgg tgagcgcctc ggacaggctg
     2941 ttcagcgtct tgttgaactg ctggcccttg ccagcaaggc cgttggcggc cgactcgatc
     3001 aggtcgccga acggaccctt cggttgatcc ggcgtcgggc cgaggccgga cagcaggttg
     3061 tcgatctcgt tccgcagctc gtcccattcg acgggtactt gagtgcgctc gatcgggatg
     3121 accgcgccat ccttcagcac gggacccccg gtgtaggccg gtgacaactg gatcgtgcgg
     3181 gaggcgacca gcgtcggatt gaggatggac gcggtcgcgt cagccggcac cttgtacttg
     3241 ttgctgtagt ggaacgtgat cttcatc
//
LOCUS       DASWBF010000185         3236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_675974,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000185 DASWBF010000000
VERSION     DASWBF010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675974"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1159)
                     /gene="trpD"
                     /locus_tag="VF874_06560"
     CDS             complement(110..1159)
                     /gene="trpD"
                     /locus_tag="VF874_06560"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX9175762.1"
                     /translation="MAGLSWQRVLSQLTSGRELAPGEAAWAMEQVMTGAATPAQIAAF
                     GVAMRMKRPTAAEVRELADTMLAHARRVPGAQIGADAVDVVGTGGDGSNSVNLSTMAA
                     IVVAANGIHVIKHGNRAASSRSGGADVLEALGVRIDLGPDDVARSVSQVGIGFCFAPL
                     FHPSFRHTSVARREIGVPTVFNLLGPLTNPAMPRAGLVGCAFEDVAEVMAGVFAERGS
                     SVLVVRGDDGLDELTTTTTSSIWRVHDGSVQRVSFDPAEFGIPKAELAHLLGGDAAEN
                     AEVARSVLAGAPGPVREAVLLNAAGAIVAHAGLAGDVEWRPAWESALQRAATAVDSGA
                     AEQLLARWAQFTQKI"
     gene            1246..1857
                     /locus_tag="VF874_06565"
     CDS             1246..1857
                     /locus_tag="VF874_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX9175763.1"
                     /translation="MTSAVGTSGTAITSRVHSLNRPNMVSVGTIVWLSSELMFFAGLF
                     AMYFTARAQAAGDWPPHPTELNLYQAVPVTLVLIASSFTCQMGVFAAERGDVFGLRRW
                     YVITFLMGAFFVAGQGYEYIHLVQHGTTIPGSAYGSVFYLATGFHGLHVIGGLIAFIF
                     LLVRTTMSKFTPAQATAAIVVSYYWHFVDIVWIALFATIYFVR"
     gene            1854..2666
                     /locus_tag="VF874_06570"
     CDS             1854..2666
                     /locus_tag="VF874_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9175764.1"
                     /translation="MRSAAVKRRRLRRRLSAGLLLLAALLLAGGLASLLTPKPQVAVA
                     DESAEALVRNGKQLYETSCVSCHGANLQGIAERGPSLIGVGEAAVYFQVSTGRMPAMS
                     QSAQAPQKPPNFDENQIDALMAYVQANGGGPMVVRDPNGQIAMESLVGDDVARGGDLF
                     RLNCASCHNFTGKGGALSSGKYAPELGGANPAQIYAAMLSGPQNMPKFSDRQLTSDEK
                     KDIIAYIKMASDTKDPGGYGLGGIGPTSEAFAMWVIGIVGVIAVALWIGSRA"
     gene            2663..>3236
                     /locus_tag="VF874_06575"
     CDS             2663..>3236
                     /locus_tag="VF874_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome C reductase"
                     /protein_id="HEX9175765.1"
                     /translation="MTNETPKVPNDRELNAMSRDELVELGTELDGVEVIYTEPRWPVE
                     GTRAEKRAERRVAFWLLLGGISGLALLVIFLFWPWEYEPDSWVYALATPLYGLTFGLS
                     ILAIGIGAVLYQKKFIPEEIAVQERHDGPSSEVDRKTIVAELAETLDASTIKRRKLIG
                     VSLGLGLGAFGLSTVVAFIGGLIKNPWKPVV"
BASE COUNT          519 a         1101 c         1080 g          536 t
ORIGIN      
        1 ctatgcggtt ctcgtcgcgt gcgcaggacc gtggacggcg tgggcgtcga cggcgcgctc
       61 ggcgaagctg gcggcgtttg atgctcttcg cgcaagcggc tcatcgccct cagatcttct
      121 gggtgaattg cgcccaacgc gcgaggagtt gttcggccgc cccggagtcg acagcggtgg
      181 cggcgcgctg caacgccgac tcccaggcgg gacgccactc gacgtcgccg gctagcccgg
      241 cgtgcgccac gatcgccccg gcggcgttga gcagcaccgc ctcccgcacc gggcccgggg
      301 cgccggctag caccgagcgt gccacctcgg cgttctccgc ggcgtcaccg ccgagcaggt
      361 gggccagctc ggccttcggg atcccgaact cggccgggtc gaagctcacc cgctgcaccg
      421 aaccgtcatg gacccgccag atcgagctcg tggtcgtggt ggtcagttcg tctaggccgt
      481 cgtcgccgcg gaccaccagc acactggacc cgcgttcggc gaatacgccg gccatcacct
      541 cggcgacgtc ttcgaacgca caacccacca ggccggcccg cggcatcgcc ggattcgtca
      601 gcggcccaag cagattgaac accgtcggca cgccgatctc gcgccgagcg accgacgtgt
      661 ggcgaaacga cgggtgaaac agcggcgcga agcagaagcc gattcccacc tgcgacacac
      721 tgcgggccac gtcgtcgggc cccagatcga tccgaactcc cagcgcctcc agcacgtcgg
      781 cgccgccact gcgtgacgac gcggcccggt tgccgtgctt gatcacgtga attccgttgg
      841 cggccacgac aattgctgcc atcgtggaca ggttgaccga gttcgacccg tcgccaccgg
      901 tgcccacgac gtcgacggcg tcggcgccga tctgcgcgcc ggggacgcga cgggcatggg
      961 ccagcatcgt gtcggcgagc tcgcggacct ctgcggcggt cggccgcttc atcctcatcg
     1021 ctacgccgaa cgcggcgatc tgggcggggg tcgccgcgcc ggtcatcacc tgctccatcg
     1081 cccaggccgc ctcaccgggg gccagctcgc gcccggaggt caactgcgag agcaccctct
     1141 gccacgacaa ccctgccacc cgccgatggt gccacgcggg aagactttgt gaccaaaagg
     1201 gtcccccgct ggaatctgac aactacaaag cgtcatactt ctgttgtgac gagcgctgta
     1261 gggacctcgg gaaccgcgat cacgtcgcga gtgcattccc tcaaccgccc caacatggtc
     1321 agtgtcggca ccatcgtctg gctttccagc gaactgatgt tctttgctgg gctgttcgcg
     1381 atgtacttca ccgcgcgggc ccaagccgcc ggcgactggc cgccgcaccc gaccgagctc
     1441 aacctgtatc aggcggtgcc ggtgacgctg gtgctgatcg cgtcgtcgtt cacctgccag
     1501 atgggcgtgt tcgccgccga gcgcggcgac gtgttcgggc tgcgccgctg gtacgtgatc
     1561 acgttcttga tgggcgcgtt cttcgtcgcc gggcagggct acgagtacat ccatctggtc
     1621 cagcacggca ccacgatccc cggcagcgcg tacggctcag tgttctacct ggccaccggt
     1681 ttccacggct tgcacgtgat cggcgggctg atcgcgttca tctttctgct ggtgcgcacc
     1741 acgatgagca agttcacccc cgcacaggcc accgcggcga tcgtcgtctc gtactactgg
     1801 cacttcgtcg acatcgtctg gattgcgctg ttcgcgacca tctacttcgt gcgatgagga
     1861 gcgctgctgt gaaacgacgc cggttacgcc gacgcctttc agccggcctg ctgctgctgg
     1921 cggcgctgct gctggccggc ggcctggcct cgctactgac acccaagccg caggtggccg
     1981 tcgccgacga atccgcagaa gcgttggtgc gcaacggcaa gcagctctac gagacgtcgt
     2041 gcgtgtcctg ccacggggcg aaccttcagg gtatcgccga gcgcggcccc agccttatcg
     2101 gcgtcggcga ggccgcggtc tacttccagg tctccaccgg gcgcatgccg gcgatgagcc
     2161 agagcgccca ggcgccgcag aagccgccga acttcgacga gaaccagatc gacgcgctga
     2221 tggcctacgt gcaggccaac ggtggcgggc caatggtcgt ccgcgacccc aacggccaga
     2281 tcgcgatgga gtcgctggtc ggcgacgacg tcgcccgcgg cggcgacctg ttccggctga
     2341 actgcgcgtc ctgccacaac ttcaccggca agggcggcgc gctgtcgtcg ggcaagtacg
     2401 cgcccgagct cggcggggcc aacccggcac agatttacgc cgcgatgctc tccggtccgc
     2461 agaacatgcc gaagttctcc gaccgccagc tcacctcgga tgagaagaag gacatcatcg
     2521 cctacatcaa gatggcgtcg gatacgaagg acccgggtgg ctacgggctg ggcggcatcg
     2581 gaccaacctc ggaggcgttt gccatgtggg tgatcgggat cgtcggtgtg atcgccgtgg
     2641 cgctgtggat tggatcgcgc gcatgacgaa cgaaacaccg aaggtgccta acgacagaga
     2701 actgaacgcg atgtcgcgtg acgagctggt ggagctcggc accgaactcg acggcgtcga
     2761 ggtcatctac acggagcccc gctggccggt ggagggcacg cgggccgaga agcgcgccga
     2821 gcggcgggtg gcgttctggc tgctgctggg cggtatctca gggctcgcgc tgctggtgat
     2881 cttcctgttc tggccgtggg agtacgagcc cgacagctgg gtgtacgcgc tggcgacccc
     2941 gctgtacggg ttgacgttcg gattgtcgat cctggcgatc ggcatcggcg cggtgctcta
     3001 ccagaagaag ttcattccgg aagagatcgc agtccaagaa cgccacgacg gcccgtcgtc
     3061 cgaggtggac cgcaagacga tcgtcgccga attggccgaa accctggacg cttccacgat
     3121 caagcggcgc aagctgatcg gggtctcgct gggcctcgga ctgggcgcgt tcgggctcag
     3181 caccgtggtg gcgttcatcg gcggcctgat caagaacccg tggaagccgg tggttc
//
LOCUS       DASWBF010000186         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1000248,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000186 DASWBF010000000
VERSION     DASWBF010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000248"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VF874_06580"
     CDS             <1..374
                     /locus_tag="VF874_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX9175766.1"
                     /translation="RADDVNGRFRELVGDEFSIKDLRTWHGTVLAAEEFAHSPEPTSK
                     TARRRTESTVMKAVAAELGNTPAVARASYVDPRVVQAFEDGLTIEPTIRRAQRHRSPA
                     ARQELIEKATCRLIHRVEKGR"
     gene            600..833
                     /locus_tag="VF874_06585"
     CDS             600..833
                     /locus_tag="VF874_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175767.1"
                     /translation="MGSELTPDDPIDEDVPVADAVDQRLEVGETPSLSETFEPTVLNE
                     IAADPAPADANPADWQEQRTVAADDDWDPAFDL"
     gene            984..1217
                     /locus_tag="VF874_06590"
     CDS             984..1217
                     /locus_tag="VF874_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX9175768.1"
                     /translation="MTENSGPKEAVRGVVEDVKGKAKEAVGTVAGRDDLVREGKAQQD
                     KAEAQRDAAKKEAEAEAARGSAKAAEKRQEANQ"
     gene            complement(1381..1692)
                     /gene="usfY"
                     /locus_tag="VF874_06595"
     CDS             complement(1381..1692)
                     /gene="usfY"
                     /locus_tag="VF874_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein UsfY"
                     /protein_id="HEX9175769.1"
                     /translation="MGDTHHDPTDHSRTYQPHAGESMIDVYFWPGFFLIAVGVVALVG
                     CVAALAYKHPELLAWAGLTAALTFGGGVSWLHVERRRVRRIEARWLAEHPVKPSHRPA
                     A"
     gene            complement(1834..2196)
                     /locus_tag="VF874_06600"
     CDS             complement(1834..2196)
                     /locus_tag="VF874_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175770.1"
                     /translation="MSPDTDDPVRDRATFAAAAKFVGIFVAIALLTLLAAMAWLSGCK
                     TGTGEGALDACNALQRNTLAVGPAVILFIGGVWAFVRTYQAWRARGGWWIWQGAGWFL
                     LVLMLVVLSMTVPPALMS"
     gene            complement(2257..2649)
                     /locus_tag="VF874_06605"
     CDS             complement(2257..2649)
                     /locus_tag="VF874_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175771.1"
                     /translation="MGVQALLKFAFFALPYRRRRAALDRSYGAEPSATTTSDTVMLLF
                     TIALIGLMLWRGIEAVSFLGGVWIGATLIQLYFHEFHAPVAEDRAAPPVVSPIKSMSY
                     AVQDSPWRPWRELVVLTVVICWSLVLLL"
     gene            2766..3188
                     /locus_tag="VF874_06610"
     CDS             2766..3188
                     /locus_tag="VF874_06610"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HEX9175772.1"
                     /translation="MDDASLDDLERKRAHLYDELAATGDFRRGSISENYRRCGKPNCV
                     CAQPDHPGHGPRYLWTRTVAGRGTKGRQLSAEEVDKVRAELANYHRFAQVSEQIVAVN
                     EAICETRPPNTATTAPPAATGDEKRGSTSRSRRRSPPR"
     gene            3272..4375
                     /locus_tag="VF874_06615"
     CDS             3272..4375
                     /locus_tag="VF874_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175773.1"
                     /translation="MSRLGASLLGQLLATDTGHRGPRIDCGTGHHAAFVGYHDKNLDT
                     VLGRVVVRRAYYHCASCGRGVVPRDDELGVAGASLSPGLRKITARAAATAPFAQAADL
                     LADLAGIRLNPKRIERSAEADGGAAAARLTTESAAIAHRRVAVLPTTARGGPDKLYIA
                     VDGTGVPMVGGAVAGRAGKAPDGRARTREVKLAALFTQTRIDDAGRPVRDPDSTSYLA
                     SFAPAAEFATLVHAEARRRGADHIRQLVVLGDGAPWIWNLATTILPEATPIVDLYHAR
                     EHLHGLAAELTGTLGDAHPDWLAARLAELDAGDIETLVTETQRLHPQLPDDIAKRHRE
                     SIGILQNQRPPDALRLLPRPRPVRRLRCRRGRL"
BASE COUNT          818 a         1525 c         1441 g          599 t
ORIGIN      
        1 cgcgcgctga cgacgtaaat ggacggttcc gggagctggt cggggacgag ttcagcatca
       61 aggacttgcg cacctggcac ggcaccgtgc tcgccgcgga ggagttcgcg cactcccccg
      121 agccgacgag caagaccgcg cgacgccgca ccgagtcgac cgtaatgaag gcggtcgcag
      181 cggaactcgg caacacgcct gcggtggcgc gagcgtcata cgtcgatccg cgggtggtgc
      241 aggccttcga agatggcctg accatcgagc cgacgattcg gcgggcccag cgccaccgct
      301 cgccggccgc ccggcaggag ctgatcgaga aggcgacctg ccggctgatc caccgcgtcg
      361 aaaaaggccg ctagttcccg gtttcgccga aacggtattc cacgtggtcg cctctcgcac
      421 tttgccgcgc tggctaccgt ctcggcggtc attccccatt tgttgaaacc tgttgcgacg
      481 aatatctttt cgttaccggg caggatcggt cctacatgcg gtgcgtcata ctccttgacc
      541 cgtcgctaaa accgcatgtc aagcgtttcg cgaggtgcgc cgtagggtac cttcgattcg
      601 tgggctccga attgacaccc gacgatccga tcgacgaaga cgttccggtc gccgacgctg
      661 tcgaccagcg cctcgaggtg ggcgaaacgc caagcctttc ggagaccttt gagccgaccg
      721 tgcttaacga gatcgcagct gaccccgcgc ccgcggacgc caatcccgcg gactggcagg
      781 aacaacgaac cgtcgccgcc gacgacgact gggatccggc cttcgacctc tgaggagggg
      841 tactccgccc gtctaaggcg gtccgacaat cgctgaggtt taacgccaga aggccagggg
      901 aagtcagacg ccagcgacgg gcccaatcgc ccgccgcggc ggatagaaac gcccacacaa
      961 ggcaagccaa caaggaggaa tccatgacgg agaacagcgg acccaaagaa gccgtccggg
     1021 gcgtcgtcga agacgtcaag ggcaaggcca aagaagctgt cgggaccgtc gcaggtcgcg
     1081 acgacttggt ccgtgagggc aaggcccagc aggacaaggc cgaggctcag cgtgacgccg
     1141 ccaagaagga agccgaagcg gaggcggctc gcggtagcgc caaggccgcc gagaagcggc
     1201 aagaggccaa ccagtagcta gttgacgcac aacggggccc ggtccaagag gactgggccc
     1261 ctttgctttt tgcgattcat aacgctcggt tgggcgcgcg ttctgccgcg ccacgcgcgc
     1321 gacccatcta cgttcggcgt acgcccacac gaaaccgccg acgccgcttg gcaactgccg
     1381 ctacgcggcg ggtcggtgtg acggcttgac gggatgctcc gccaaccacc gggcctcgat
     1441 gcgccgcacc cgacgacgtt cgacgtggag ccaagaaact cccccgccga aggtcagggc
     1501 ggcggtcagg ccggcccagg ccagcagttc cgggtgtttg tacgcgagtg ccgccacgca
     1561 gccgaccagc gcgacgacgc ccaccgcgat caggaagaag cctggccaaa agtagacgtc
     1621 gatcatcgac tcaccggcgt gcggttgata agtgcgggag tgatccgtgg gatcgtgatg
     1681 cgtgtctccc atcacagtct ccttcgtcga gataccccaa ggatactcgc tgcaggccgc
     1741 gatgggagaa ccagaaatac gttctgacca gccgattact tgtacggcgg gtacttgtgc
     1801 tggcgcagca cgttggcgac gatgcgaaaa cggctaagac atcagggccg gcgggacggt
     1861 catcgacagc accaccagca tcagcacgag caggaaccag ccggcgccct gccagatcca
     1921 ccacccacca cgggcgcgcc acgcctggta tgtgcggacg aacgcccaca cgccgccgat
     1981 gaacaggatg accgcgggac cgacggccag tgtgttgcgc tgcagtgcgt tgcaggcgtc
     2041 gagcgctcct tcgccagtgc ccgtcttgca gccgctcaac cacgccatcg cggcgagcag
     2101 ggtcagcagg gcgatcgcga cgaagatgcc gacgaacttc gcggccgccg cgaacgttgc
     2161 gcggtcacgc accggatcgt ctgtgtcagg cgacattttc atgtgctacc cgaccctacg
     2221 ctcgtcgcgt ccgcagtaac tcgggccgcg cgctgatcag agaagaagca cgaggctcca
     2281 gcagatcacg acggtcaata cgaccagctc ccgccacggc cgccacggcg aatcctggac
     2341 cgcgtacgac atcgacttga tcggcgagac gaccggcggc gccgcgcggt cctctgcgac
     2401 gggcgcatga aactcgtgga aatacaactg gatcagcgtg gctccgatcc acacgccgcc
     2461 caggaagctc actgcttcga tcccccgcca cagcatcaac ccgatcaggg cgatcgtgaa
     2521 cagcagcatc acggtgtcgg aggttgtggt ggcggacggc tcggcgccgt agctgcggtc
     2581 cagcgccgca cggcgcctgc ggtacggcaa cgcgaaaaac gcgaacttca gcagggcctg
     2641 cacgcccaac agggcgacga gaacggtggt gacggtgggt gtgcgacacg actgtagggg
     2701 acgggggttg aggctggtcg cgagatattc tgggtgtggt ataaacaacc agacaggaga
     2761 tcggaatgga cgacgcctcg ctggatgatc tggaacggaa gcgggcacac ctttacgacg
     2821 agttggccgc gaccggcgac ttccggcgcg gctcgatcag cgagaactac cgtcgctgcg
     2881 gcaagcccaa ttgcgtgtgc gcgcaaccgg atcaccccgg ccacgggccg cggtatctgt
     2941 ggacccgcac ggtggccggg cggggcacca agggtcggca gctatcggcc gaggaggtgg
     3001 ataaggtgcg cgccgagctg gccaactacc accgcttcgc gcaggtgtcc gagcagatcg
     3061 tggcggtcaa cgaggcgatc tgcgagaccc ggcccccgaa tacggcgacc acggcgcccc
     3121 cggccgcgac aggggacgaa aaaaggggct ctacgagcag atcgcggcgg aggtcgccgc
     3181 cgaggtagag cgactggccg cggtcgcggt cgactcgctg ggcccctcgg gtgcgggcct
     3241 ggccgcggtc gagctggcga tccgcaccgc gatgtcccgg ctcggcgcat cgctgctggg
     3301 gcagctattg gccaccgaca ccgggcaccg cgggccgcgg atcgactgcg ggaccgggca
     3361 tcacgcggcc ttcgttggct accacgacaa gaacctcgac accgtgttgg gccgtgtcgt
     3421 ggtgcgccgc gcctactacc actgcgcctc gtgcgggcgc ggggtcgtcc cgcgcgacga
     3481 cgagctcggc gtcgccggcg cctcgctgtc accgggactg cgcaagatca ccgcgcgcgc
     3541 cgccgcgacc gcgccgttcg cccaagccgc cgacctgctg gccgacctgg ccggaatccg
     3601 gttgaacccc aagcggatcg aacgatcggc cgaagccgac ggcggcgccg ccgcggcacg
     3661 gctcaccacg gaatcggccg cgatcgcgca ccggcgggtg gccgtgctgc ccaccaccgc
     3721 gcgcggcggg ccggacaagc tctacatcgc cgtcgacggc accggtgtgc ccatggtggg
     3781 cggcgcggtc gccggccgcg ccggcaaggc acccgacggg cgggcccgca cccgcgaggt
     3841 caaactcgcc gccctgttca cccagaccag gatcgacgac gccgggcgac cggtccgtga
     3901 ccccgactcc accagctacc tggccagctt cgcccccgcg gccgagttcg ccacgctggt
     3961 gcacgccgag gcccgccgcc gcggcgccga ccacatccgc caactcgtgg tcctcggcga
     4021 cggcgcgccc tggatctgga acctggccac cacgatcctg cccgaggcca cccccatcgt
     4081 ggacctctac cacgcccgcg aacacctgca cggcctcgcc gccgagctca ccggcacact
     4141 cggcgacgcc caccccgact ggctggccgc ccgcctggcc gagctcgacg ccggcgacat
     4201 cgaaaccctc gtcaccgaaa cccagcgtct gcacccacag ctgcccgacg acatcgccaa
     4261 acgacaccgc gaaagcattg ggatacttca aaaccaacgc ccaccggatg cgctacgcct
     4321 acttccgcgc ccacggcctg ttcgtcggct ccggtgtcgt cgaggccggc tgtaaatcca
     4381 tcg
//
LOCUS       DASWBF010000187         8715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_743054,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000187 DASWBF010000000
VERSION     DASWBF010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743054"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VF874_06620"
     CDS             <1..849
                     /locus_tag="VF874_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175774.1"
                     /translation="VISGAGDRAAASELLDQQVKPFVDNDIRVDWLTAVRTATASIGS
                     SYDAATAGLTSAPPVQFDVPGDLGPESAPSPRVDDEAAAGDPAPTTRAAAAPSPAPPP
                     PPSSPAYPVMPAMAPTQMPAAMPAGLGGSPATAAPAPTAAPTGLDGLGQRFADALGNL
                     GGLVPSPGDTALPDQPALEPPTLGDPPSDPSAHDDANDEDVDKLGDVDPDGETDCPGS
                     EHKEGEPAPAADVDTGPPPVEPVPPVDPAEPPAPPPAPPAPEAPAPEAGTPCEIAADE
                     LPQAGQ"
     gene            846..1019
                     /locus_tag="VF874_06625"
     CDS             846..1019
                     /locus_tag="VF874_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175775.1"
                     /translation="MTDVNQRLADILADYSDTMSRIERSSSDVHDVPYGGPVLKPADQ
                     QQSCRAPTFGPDR"
     gene            complement(1022..1873)
                     /locus_tag="VF874_06630"
     CDS             complement(1022..1873)
                     /locus_tag="VF874_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175776.1"
                     /translation="MARTRKLFASLTRRGPHRVLRGDLAFAGLPGIVYTPESGFNLPA
                     VVFGHDWLTAADRYRRTLEHLASWGIVAAAPATERGVAPSALNFAHDLGTTLDIVAGV
                     RLGPGKISVHPTKLGLVGHGFGGSAAVLAAAGRSGSRFAPKTVAALFPAVTSPPAEKA
                     AATLTIPGLVLTAPGDPHTLRSNAVDVAREWDGAVLRIVSKGKPGGLGEGRRLARVVG
                     LPGADRSTQKSVRALLTGYLLFVLAGDKTYRDFADPDAQLPKTEPVDPDADPVDVEDK
                     VIALLKS"
     gene            1929..3797
                     /gene="glmS"
                     /locus_tag="VF874_06635"
     CDS             1929..3797
                     /gene="glmS"
                     /locus_tag="VF874_06635"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX9175777.1"
                     /translation="MCGIVGYVGHRRACDVVVQALHRMEYRGYDSAGIAILDGNGGMT
                     VERRAGRLANLGEALDCSDPVSLQGHSGLGHTRWATHGRPTDRNAHPHRDATGKIAVV
                     HNGIIENFATLRRELEAAGVEFASDTDTEVAVHLVGRQYRFGDTAGDFVASVLAVLRR
                     LEGHFTLVFANADDPGTIVAARRSTPLVLGVGDGEMFVGSDVAAFIEYTRDAVELGQD
                     QAVVITADGYRVTDFSGEPDDANARAFHIDWDLSAAEKGGYEYFMLKEIAEQPAAVAD
                     TLLGHFVDGRIVLDEQRLSDQDLREVDKVFVVACGTAYHSGLLAKYAIEHWTRLPVEV
                     ELASEFRYRDPVLDRSTLVVAISQSGETADTLEAVRHAKEQKAKVLAICNTNGSQIPR
                     ECDAVLYTRAGPEIGVASTKTFLAQIVANYLVGLALAQARGTKYPDEVAHEYHELEAM
                     PALVSRVLANIEPFADVARRFATASTVLFLGRHVGYPVALEGALKLKELAYMHAEGFA
                     AGELKHGPIALIEDGLPVIVVMPSPKGSAMLHAKLLSNIREIQARGAITIVIAEEGDD
                     TVRPYADHLFEIPAVSTLFQPLLSTIPLQVFAASVAQARGYDVDKPRNLAKSVTVE"
     gene            complement(3890..4210)
                     /locus_tag="VF874_06640"
     CDS             complement(3890..4210)
                     /locus_tag="VF874_06640"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEX9175778.1"
                     /translation="MTEIDADLEGPISAPRNNGEIVFEAPREQRVFGLAVALCRSQTC
                     DWESFRQRLIRHITDDADLPYWQNWALALEDVLAETSIVTPSELDVRHRKLLERPAGH
                     DHHR"
     gene            complement(4207..4572)
                     /locus_tag="VF874_06645"
     CDS             complement(4207..4572)
                     /locus_tag="VF874_06645"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX9175779.1"
                     /translation="MNDRGEARVAALEALLVAKGVVDPAAVDRVIEHYESDVGPLNGA
                     KVIARAWTDPAYRQRLLADGTRAVAEFGFGGPEAQMLVAVENTRAPAGRRPARRGAAG
                     ALGHPRLDDRRWPRAESHR"
     gene            complement(4600..4923)
                     /locus_tag="VF874_06650"
     CDS             complement(4600..4923)
                     /locus_tag="VF874_06650"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175780.1"
                     /translation="MHDMGGRTEFFGPVVEEADEPVFHEPWEGRVFGLSMFLMPLLGR
                     NVDAFRFAQERPPRDVYLSGSARMSFPIRTPSVVGEDRSTSTPSPSKRLICGGTARSR
                     APRCG"
     gene            5018..5371
                     /locus_tag="VF874_06655"
     CDS             5018..5371
                     /locus_tag="VF874_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9175781.1"
                     /translation="MSKVSVREFSYNPSAMFARAEKGETIEITRHGKVIARLIPGQQK
                     KRSRIEELVESGALRMSEKTLADIDTFTRIKLPEGAPDPLETLLEERYTESEWEREFR
                     EEWEARRGASGDAKS"
     gene            5368..5763
                     /locus_tag="VF874_06660"
     CDS             5368..5763
                     /locus_tag="VF874_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9175782.1"
                     /translation="MIYLDASALVTYVTERPYFPELHDFLIARSAFRGVTSTVGLIET
                     VRNCDRIGNFPDLMTQLLREYAELHITSEIRDRAANVPGGLGALDAIHMATAETLGEE
                     LISFVTYDRRMAMVARSRGLPVASPGMKS"
     gene            5827..7248
                     /locus_tag="VF874_06665"
     CDS             5827..7248
                     /locus_tag="VF874_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX9175783.1"
                     /translation="MRHYYTADAIRQAEAPLLASMPEGALMRRAAYPLSVAVARELAA
                     RTGGVASRRICAVVGSGDNGGDALWASTFLRRRGAAASAVLLNPEKTHKKGLAAFIRA
                     GGRVVESVPPSTDLVIDGVVGISGSGPLRPAAADVFAAVDEAGIPVVAVDSPSGVVVS
                     TGAASGPHVHAAVTVTFGGLKAVHALGDCGRVELVDIGLELPPTDLLGFEAADVRERL
                     PLPGPRDDKYTQGVTGILAGSATYPGAAVLSTGAAVAATSGMVRYAGSAAAQVLSRWP
                     EVIASPTAESAGRVQAWVVGPGIGTDDAGAGALWFAMGTDLPVIVDADALTILAAHPD
                     AVASRSAPTVLTPHAGEFARLAGSPPGDDRVGATRALADKFGATVLLKGNVTVIADPG
                     GPVYLNSAGGSWAATAGSGDVLSGMIGALLAHGLPAGEAAAAAAFVHARAANLSAADP
                     GPKPTPTSASRILAHIRSALAAL"
     gene            7263..8648
                     /locus_tag="VF874_06670"
     CDS             7263..8648
                     /locus_tag="VF874_06670"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEX9175784.1"
                     /translation="MPRVSHPSPSIAPAYTGRLSTAPIPSLRLPDEPMDPDAAYRFIH
                     DELMLDGSSRLNLATFVTTWMDPQAEKLMAETFDKNMIDKDEYPATAAIEQRCVCMVA
                     DLFHAEHLRDDDPSSAVGVSTIGSSEAVMLGGLALKWRWKERVGKAWKTRTPNLVMGS
                     NVQVVWEKFCRYFEVEPRYLPMAEGRYTITPEQVLDAVDEDTIGVVAILGTTYTGELE
                     PIAEICAALDKLAATAGNPDVPVHVDAASGGFVVPFLHPDLEWDFRLPRVVSINVSGH
                     KYGLTYPGIGFVVWRNSDCLPDDLVFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNFLR
                     LGRAGYGQVMGCLSTTARWLSDQLRKTRHFEVIADGSAIPVVAFRLAGDFGYTEFDVS
                     AGLRSYGWQVPAYTMPEGAENVAVLRVVVREGFSADLARSLFEDMAKVVAHMDELKPG
                     GHFDQQQHFAH"
BASE COUNT         1400 a         3061 c         2966 g         1288 t
ORIGIN      
        1 gtcatctccg gcgccggcga ccgcgcggca gcaagtgaac tgcttgacca gcaagtaaaa
       61 cccttcgtag acaacgacat tcgcgtcgac tggctgacag cggtacgtac ggcgaccgcg
      121 tcgatcggca gcagttacga cgccgccacc gccgggttga cgtccgcgcc gccggtgcag
      181 ttcgacgtcc cgggagacct cggtccggaa tcggcaccgt cgccgcgcgt ggacgacgag
      241 gcggcagccg gcgatccggc accaaccacc cgagccgccg cggcgccgtc acccgcgccg
      301 ccgccgccgc cgtcgtcgcc ggcgtatccg gtcatgccgg cgatggcgcc gacgcagatg
      361 cctgccgcga tgcccgccgg tctcggtggc agtcccgcaa ccgccgcacc cgcgccgacg
      421 gccgccccca ccggactgga cgggcttggt cagcggttcg ccgacgcgct gggcaacctg
      481 ggcggcctgg tgccgtcacc gggcgacacc gcgctgcccg accagccggc gctggagccg
      541 cccacgctcg gtgacccgcc gagcgatccg tcggcgcacg acgacgcgaa cgacgaggac
      601 gtcgacaagc tcggcgacgt cgaccctgac ggcgaaaccg attgccctgg aagcgaacac
      661 aaggagggtg aaccagcccc cgctgccgac gtcgacactg gcccgccgcc cgtcgagcca
      721 gtgcctccgg tcgaccccgc ggaaccgccg gcacccccgc ctgcgccacc ggcacccgag
      781 gcgcccgcac ccgaagcggg cacaccgtgt gagatcgccg ccgacgagct accgcaggcc
      841 gggcaatgac cgacgtcaac cagcggctgg ccgatatcct cgccgactac agcgacacga
      901 tgagccggat cgagcgctcg tcatcagatg tgcacgatgt gccctacggc ggcccggtac
      961 tgaaacccgc cgaccagcag cagtcctgca gggcgccgac cttcggcccg gaccgctgac
     1021 gtcaggattt gagaagcgcg atgaccttgt cctcgacgtc gaccgggtcg gcgtcggggt
     1081 cgacgggctc ggtcttcggc agctgcgcgt cggggtcggc gaagtcgcgg taggtcttgt
     1141 ccccggccag cacaaacagc aggtatccgg tcagcagcgc ccgcacagac ttctgcgtgc
     1201 tgcggtcggc accaggaagc ccgactaccc gagccagccg gcgcccctcg cccagcccgc
     1261 cgggtttgcc cttgctgacg atcctcaaca cggcgccgtc ccattcgcgg gccacgtcca
     1321 cggcgttgct gcgcagagtg tgcgggtcgc cgggtgcggt gagcaccaga cccgggatcg
     1381 tcagcgtcgc ggccgccttc tcggccggtg ggctggtgac ggccggaaac agcgccgcca
     1441 cggtcttcgg cgcgaaccgc gagccgctac gtcccgccgc ggccagcacc gcagccgagc
     1501 cgccgaagcc gtgcccgacc agacccagct tggtggggtg cacgctgatc tttccggggc
     1561 ccagccgcac accggcaacg atgtcgagcg tcgtgcccag gtcgtgggcg aagttcagcg
     1621 ctgacggcgc caccccgcgc tcggtggccg gagccgcggc cacgattccc caggacgcca
     1681 gatgctcgag cgtgcggcgg taccggtcgg cagctgtcag ccagtcgtga ccgaacacca
     1741 cggcaggcag attgaagccc gattccgggg tgtagacgat gcccggcagt ccggcaaagg
     1801 ccaggtcacc gcgcagaacg cgatgaggac cgcgacgggt gagcgaggcg aacagcttgc
     1861 gtgtccgggc catgggtgaa ccgtagctga accgctccgg tcccggccct gcactaccct
     1921 ggacacctat gtgcggcatc gtcggctacg tcgggcaccg tcgcgcctgc gatgtggtcg
     1981 tgcaggcgct gcaccggatg gagtaccgcg gctacgactc ggcgggcatc gcgatcctcg
     2041 acggcaacgg cggaatgacg gttgagcgcc gcgcgggcag gctggccaac ctgggcgagg
     2101 cgctcgattg cagcgacccg gtgtcgcttc aggggcactc aggcctcggc cacacccgct
     2161 gggcgaccca cggccgtccg accgaccgca acgcccaccc gcaccgcgat gccaccggca
     2221 agatcgcggt cgtgcacaac ggaatcatcg agaacttcgc gacgctgcgc cgggaactgg
     2281 aggccgccgg ggtcgagttc gccagcgaca ccgacaccga agtggccgtg cacctggtgg
     2341 gccgccagta ccgcttcggc gacaccgccg gcgacttcgt cgcatcggtg ctggcggtgc
     2401 tgcgccggct cgagggccac ttcacgctgg tgttcgccaa cgccgacgac cccggcacca
     2461 tcgtggcggc caggcggtcg acgccgctgg tgctcggcgt cggcgacggg gagatgttcg
     2521 tcggctcgga cgtggccgcg ttcatcgagt acacccgcga cgccgtggaa ctcggtcagg
     2581 accaggccgt ggtgatcacc gccgacggct accgcgtcac cgacttctcc ggcgaaccgg
     2641 acgacgcgaa tgcccgggcc tttcacatcg actgggacct gtccgccgcg gaaaagggcg
     2701 gctacgagta cttcatgctc aaggagatcg ccgagcagcc ggccgcggtc gccgacacgc
     2761 tgctcgggca cttcgtggac ggccgcatcg tgctcgacga gcagcgcctc agcgaccagg
     2821 atctgcgtga ggtcgacaag gtcttcgtcg tcgcgtgcgg caccgcgtac cactccgggc
     2881 tgctggccaa gtacgcgatc gagcactgga cccggctgcc cgtggaggtc gagctcgcca
     2941 gcgagttccg ctaccgcgat cctgtgctgg accgcagcac gctggtggtc gcgatctcac
     3001 agtccggcga gaccgccgac acgctggagg cggtgcgcca cgccaaggag cagaaggcca
     3061 aggtgctggc gatctgcaac accaacggca gccagatccc ccgcgagtgc gacgcggtgc
     3121 tctacacccg cgccgggccg gagatcggcg tcgcgtccac caagacgttc ctggcccaga
     3181 tcgtggccaa ctacctcgtc ggtctcgcgc tcgcacaggc gcgaggcacg aagtatcccg
     3241 acgaagtggc acacgagtac cacgaacttg aggcgatgcc ggcgctggtg tcgcgggtgc
     3301 tggccaacat cgagccgttc gctgacgtcg cgcgccggtt cgcgacggcg tcgaccgtgc
     3361 tgttcctcgg tcgccacgtc ggctacccgg tggcgctgga aggcgcgctg aagctcaagg
     3421 aactggccta catgcacgcc gagggttttg cggccggcga gctcaagcac gggccgatcg
     3481 cgctgatcga ggacggcctg ccggtgatcg tggtgatgcc gtcgccgaag ggctcggcga
     3541 tgctgcacgc caagctgctg tccaacatcc gcgagatcca ggcgcgcggc gcgatcacga
     3601 tcgtcatcgc cgaagaaggc gacgacaccg tacgccccta cgccgaccac ttgttcgaaa
     3661 tcccggctgt gtcaacgctt ttccagccgc tgctgtcgac gattccgctg caagtgttcg
     3721 ccgcgtcggt ggcgcaggcc cgcggctacg acgtcgacaa gccccgcaac ctggcgaagt
     3781 ccgtcacggt cgagtaggcg cctagcttcg tcaccgattt ctgcgtcagg gctgtgactg
     3841 atgctgatcc acagccctgg tgcagatctc gacgaataga ccgccaaggt caccggtgat
     3901 ggtcgtgtcc ggccgggcgc tccagcagtt tgcgatgccg gacatcgagc tcggacggcg
     3961 tgacaatcga cgtctcggcg agcacgtctt cgagcgcgag cgcccagttc tgccagtagg
     4021 gcaaatcggc gtcgtcggtg atatgccgaa tcagcctctg ccgaaagctt tcccagtcac
     4081 aggtctggga acggcacagg gcgacggcga gcccgaacac ccgctgctcc cgcggcgctt
     4141 cgaagacgat ctccccgttg tttcgtggcg cagagatcgg cccctcgagg tcggcgtcga
     4201 tctcagtcat cggtgggatt cagcgcgcgg ccaacgccga tcatcgagtc gcgggtgacc
     4261 aagcgcgcca gctgcgcctc gtcgagccgg acggcgcccg gcgggcgctc tggtgttctc
     4321 gacggcgacg agcatctgcg cttcgggacc gccgaacccg aactcggcga cggcccgtgt
     4381 cccgtcggcg agcagccgct gccggtacgc cgggtcggtc catgcccggg cgatcacctt
     4441 ggcgccgttg agcgggccga cgtcggactc gtagtgctcg atgaccctgt cgacggcggc
     4501 agggtcgacg acacctttcg ccaccagcag cgcctcgagt gccgctacgc gggcctcccc
     4561 cctgtcgttc acgtcgcctc caggtaaggc tcgtaaggtc tacccgcatc tcggtgcccg
     4621 gctccgcgcg gtccccccac agatcagccg cttcgaaggc gacggtgtag aggtgctgcg
     4681 gtcttccccg acgaccgacg gcgtgcgtat cgggaaagac attcgtgccg aacccgacag
     4741 gtagacgtcg cgcggcggcc gctcctgggc gaaccggaag gcgtcgacgt tgcggccgag
     4801 gagcggcatc aggaacatgg acagtccgaa gactcggccc tcccacggct cgtggaagac
     4861 cggctcgtcg gcctcttcga cgaccgggcc gaagaactcg gttcgcccgc ccatgtcatg
     4921 catggccgcc acggcgcgaa cataacaccg gcccatttgt aggtacaatg atgtacgtac
     4981 ggtgtaggta catataactc gtaggaggtt actaagcgtg agcaaggtgt cggtgcgcga
     5041 gttctcctac aaccccagcg cgatgttcgc ccgcgcggaa aagggcgaga ccatcgagat
     5101 cacccgtcac gggaaggtca tcgccaggct tatccctggc cagcagaaaa agcggagccg
     5161 gatcgaggag ctcgtggagt cgggggcgct gcggatgtca gaaaagacgc tggccgacat
     5221 cgacacgttc acgcgtatca agcttcccga gggcgctccc gatccgctcg agacccttct
     5281 cgaggagcgc tacaccgaaa gcgaatggga gcgagagttc agagaagagt gggaggcccg
     5341 acgcggcgcg tcaggcgacg caaagtcgtg atctatctcg atgcctcggc gttagtcacg
     5401 tacgtgaccg aacgaccgta cttccccgag ctgcacgact ttctgatagc tcgttcggcc
     5461 tttcgcggcg tgaccagcac cgtgggattg atcgagacgg tgcgcaattg cgatcgcata
     5521 gggaacttcc ctgatctcat gacccagctg ctgcgtgagt acgccgagct tcatatcacc
     5581 tctgaaatcc gcgacagggc agccaacgtg ccgggtggtc tgggggcgct cgacgccatc
     5641 cacatggcta ccgcggaaac gctgggcgaa gaactgatct cgttcgttac ctacgaccga
     5701 cgaatggcga tggtggcgcg gtcgaggggg cttcccgtcg cgtcgcccgg catgaaatcc
     5761 tgacctcggg catccgctgc cgcgtaacgt attgccgcgc gggcacgtcg accggaacgg
     5821 gagctgatgc ggcactacta caccgctgac gcgatccgtc aggccgaggc gccgctgctg
     5881 gccagcatgc ccgagggtgc gctgatgcgc cgcgcggcct acccgctgtc cgtcgcggtg
     5941 gcccgcgaac tggcggcccg caccggcggc gtcgcgagcc ggcgcatctg tgcggtggtg
     6001 ggatccggcg acaacggcgg cgacgcattg tgggcgtcga cgtttctgcg ccgccgggga
     6061 gcggccgcct cggcggtgct gctcaacccg gagaagacgc acaagaaagg cctggccgca
     6121 ttcattcgcg ccggcggccg ggtagtcgaa tccgtcccgc cctcaacgga tctcgtgatc
     6181 gacggtgtcg tcggcatctc cggctccggg ccgctgcgtc ccgctgccgc cgacgtgttc
     6241 gccgccgtcg acgaggccgg catcccggtc gtggccgtcg acagccccag cggagtggtc
     6301 gtcagcaccg gcgccgcgag cggcccgcac gtgcacgcgg cggtgaccgt cacgttcggc
     6361 ggactgaaag ccgtgcacgc gctcggcgac tgcgggcgcg tcgaactcgt cgacatcggg
     6421 ctggagctcc cgcccaccga cctgctcggc ttcgaggccg ccgacgttcg ggagcgcttg
     6481 ccgctgcccg gcccgaggga cgacaagtac acccagggcg tcaccggcat cctggccggc
     6541 tcggcgacgt accccggcgc agccgtactc agcaccgggg cggccgtcgc agccacctcc
     6601 ggcatggtcc gctatgcggg aagcgctgcc gcacaagtac tttcgcgctg gccggaagtc
     6661 atcgcctcgc ccaccgccga gtcggccggg cgagtgcagg cctgggtggt cggacccggg
     6721 atcggcaccg acgacgccgg cgccggcgcg ttgtggttcg cgatgggcac cgacctgccg
     6781 gtgatcgtcg acgccgacgc gctgaccatc ctggccgctc accccgacgc cgtcgcgtcg
     6841 cggtccgcgc cgacggtgct tacgccgcat gcgggggagt tcgcccggct ggcaggctcg
     6901 ccgcccggcg acgaccgggt cggggccacg cgggcgcttg ccgacaagtt cggcgcgacc
     6961 gtgctgctga agggcaacgt gaccgtgatc gccgatcccg gcggtccggt gtacctcaat
     7021 tccgcgggcg ggtcgtgggc ggccaccgcc gggtccggcg acgtgctgtc cgggatgatc
     7081 ggcgcgctgc tggcccacgg actgcccgcc ggagaggccg ccgccgcggc cgcgttcgtc
     7141 cacgcccgcg ccgcgaacct gtcggccgcc gaccccggcc ccaaaccgac ccccacgtcg
     7201 gcgtcgcgca tcctcgccca catccgctcc gccctcgctg ctctgtgacg aaaggacctg
     7261 ccatgccccg tgtttcgcat ccgtcgccct cgatcgcccc ggcctacacc gggcggctgt
     7321 ccaccgcgcc gatcccgtcg ctgcggctgc ccgacgagcc gatggacccc gacgcggcgt
     7381 accggttcat ccacgacgag ctgatgctcg acggcagctc gcggctgaac ctcgcgacgt
     7441 tcgtcaccac ctggatggac ccgcaggccg agaagctgat ggccgagacg ttcgacaaga
     7501 acatgatcga caaggacgaa tacccggcca ccgcggcgat cgagcagcgc tgcgtgtgca
     7561 tggtcgccga cctgttccac gccgagcacc tgcgcgacga cgacccgtcc agcgcggtcg
     7621 gcgtctccac gatcgggtcc agcgaggccg tcatgctcgg cgggctggcg ctgaagtggc
     7681 gatggaagga acgagtagga aaggcgtgga agacgcggac ccccaacctg gtgatggggt
     7741 ccaacgtgca ggtggtctgg gagaagttct gccgctactt cgaggtcgag ccgcggtacc
     7801 tgccgatggc cgagggccgc tacaccatca cccccgagca ggtgctggac gccgtcgacg
     7861 aggacaccat cggcgtcgtc gcgatcctcg gcaccaccta caccggcgag ctggagccga
     7921 tcgccgagat atgcgcggcg ctggacaaat tggcggcgac cgctggcaat cccgacgtgc
     7981 cggtgcatgt cgacgcggcc agcggcgggt tcgtcgtccc gttcctgcac cccgacctcg
     8041 agtgggactt ccggctgcca cgggtggtgt cgatcaacgt cagcggccac aagtacgggc
     8101 tgacgtaccc gggcatcggg ttcgtggtgt ggcgcaacag cgactgtctg cccgacgacc
     8161 tggtgttccg cgtcaactac ctcggcggcg acatgccgac gttcacgctg aacttctccc
     8221 ggccgggcaa ccaggtcgtc ggccagtact acaacttcct gcggctcggc cgggcgggct
     8281 acggccaggt catgggatgc ctgtcgacga cggcgcgctg gctgtccgac cagctgcgca
     8341 agaccaggca cttcgaggtg atcgccgacg ggtcggcgat cccggtggtg gcgttccggc
     8401 tggccggcga cttcggctac accgagttcg acgtgtccgc gggcctgcgg tcctacggct
     8461 ggcaggtccc ggcctacacc atgcccgagg gcgccgagaa cgtggccgtg ctacgggtgg
     8521 tggtccggga gggcttctcg gccgacctgg ccaggtcgct gttcgaggac atggcgaagg
     8581 tggtcgccca catggacgag ctcaaaccgg gcggccattt cgaccagcaa cagcacttcg
     8641 cgcactgagt tggagctggc gctcgcgaaa aacctgatgt agatcatggt ctacaccggg
     8701 tagcctggac gcatg
//
LOCUS       DASWBF010000188        11777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_603290,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000188 DASWBF010000000
VERSION     DASWBF010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603290"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1023)
                     /locus_tag="VF874_06675"
     CDS             complement(205..1023)
                     /locus_tag="VF874_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9175785.1"
                     /translation="MIAELSRLVALSPDGSLGRINALVRQTCASVLRLPPLPAPDEPA
                     SEPAVVEFAEQFSVDVSGISDDQRAWLLSTLGGDAFGAVVLTYIADFVPRVLAGLRVL
                     GLGGVVPTGPTDWDHTTDPADAVLNAFLPAVARETALDPVTSEIVRLRGAGQHHCRLC
                     NSLREATALEAGGSEDLYGDISLYEASNRLTDAHKAALRYVDALIWSPADIAPSIAAE
                     VRACFSDAEAVEITFDVMRNAANKIAVAMAADAPRIESGTERYRILADGQTQYQ"
     gene            complement(1034..2395)
                     /locus_tag="VF874_06680"
     CDS             complement(1034..2395)
                     /locus_tag="VF874_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX9175786.1"
                     /translation="MIAPQQVVDEALAAAKVSETIVIVTDRAEASLRWANNSMTTNGV
                     SSSRSMSVISVVRDFDKAHVGSVQTSEVDPAVIRDVVAASEDAARAAPEARDSAPLIT
                     GSDEPGDWDLAVAATGAEAFGALAASLARAFRGPDTLYGYARHEMATTFVASSTGVRR
                     RFTQPTGSVEINAKRDGASAWAGVSTPDFLDVPVDRLLSDLSTRLGWSRRTLSLPAGR
                     YPTLMPPSTVADMMIYMAWMMDGRGAQEGRTAFSAPGGGTRVGERLTQLPVTLYSDPF
                     VAPLQCRPFVATSSSSDTVSVFDNGMDVDRVDWIRDGVINALAYPRAAAAEFGAPVAV
                     PADNLLMTGGDASLEDMIGQTERALLLTTLWYIRTVDPTNLLLTGLTRDGVYLVEDGK
                     VSAAVNNFRFNESPLDLLGRATEVGATEVTLPREWGDWATRAAMPPLMIPDFYMSSVS
                     QAQ"
     gene            complement(2392..3906)
                     /locus_tag="VF874_06685"
     CDS             complement(2392..3906)
                     /locus_tag="VF874_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9175787.1"
                     /translation="MAARGIDADFLGLPRRELADAALSAATEAGASHADLRIHRIVTE
                     VIHLRDAMLETSVISREVGLAVRVIVDGTWGFASHAELAPEVAAETARRAVQVARTLR
                     ALNAEDVRLAEEPVYCDAEWVSSYQTDPFDVAAADKIEMLQEYSDRLLSADGVDHVSA
                     GFHGVKEQTFYADVYGSSITQQRVRVQPTLEAVAVDAAAGSFDSMRTLAPPTARGWEA
                     VVDDGIWNWSDELAEIPSLLTEKVKAPTVQPRPTDLVIDPSNLWLTIHESIGHATEYD
                     RAIGYEAAYAGTSFATPDKLGTLHYGSPVMNVTADRCVDYGLATVGYDDEGVAAQNWD
                     LVRDGVFVGYQLDRVFAPRLGEERSNGCSYADSPHHVPIQRMANVSLQPGHEDTSTED
                     LIARVEDGLYIVGDKSWSIDMQRYNFQFTGQRFWSIRNGELDGQVRDVAYQATTTDFW
                     GSLEAVGGESTWRLGGAFNCGKAQPGQVAAVSHGCPSALFRGVRVLNTMTEAGH"
     gene            4069..4437
                     /locus_tag="VF874_06690"
                     /pseudo
     CDS             4069..4437
                     /locus_tag="VF874_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            4434..5003
                     /locus_tag="VF874_06695"
                     /pseudo
     CDS             4434..5003
                     /locus_tag="VF874_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(5000..5398)
                     /locus_tag="VF874_06700"
     CDS             complement(5000..5398)
                     /locus_tag="VF874_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEX9175788.1"
                     /translation="MILIDTSAWVEYFRATGSPAAVNVRILLAKRAEDVAMCEPVAME
                     ILAGAGDDTDGKLERLVNGLPSLTVEPAVDFRAAATIYRAARRGGRKIRSLNDCLIAA
                     IAIRHEAEVVHRDADFDAIASVVPLAATYY"
     gene            complement(5395..5607)
                     /locus_tag="VF874_06705"
     CDS             complement(5395..5607)
                     /locus_tag="VF874_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9175789.1"
                     /translation="MSRTNIELDDRLVDEVMRRFGVSTKREAVDLALRRLVGTPLTKE
                     FLLSLEGVGWNGDLDEIRSEPPADLA"
     gene            complement(5649..6476)
                     /locus_tag="VF874_06710"
     CDS             complement(5649..6476)
                     /locus_tag="VF874_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEX9175790.1"
                     /translation="MSTPTQRRPVVLLRPIPGTSLIHELWAGTKLIAAFGISVLLTFY
                     PGWVPIGLVAVLVLTAAWIARIPGSALPTVPLLLWIIVALGGVTAALGGGPPDIHIGS
                     ATLGVGGLLYFLRITALSIVLLGIGGMVSWTTNVADVAPAVATLGRPLRWLRIPVDEW
                     AAALGLALRAFPMLIDEFRVLYAARRLRPRPVLTTRRARRRWWRAELIDMVTAAITVA
                     LRRADEMGDAIAARGGTGQISAAPSRPGWADAWTLLIVAAVSAAAIASELLLGSTRF"
     gene            complement(6473..8440)
                     /locus_tag="VF874_06715"
     CDS             complement(6473..8440)
                     /locus_tag="VF874_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9175791.1"
                     /translation="MAALSAATAIVAVVIPFAAGLSLLGTVPMGLLAYRFRFRVLIAA
                     TIAGGLIAFLVAGMGGMMTVVECAYIGGLIGLVKRKRRGTPTIIVVALVAGLAFGVIT
                     VVALSVLSRLRNLMFESMTANVNGLARFLSHLPYMDPVADFLKRGLATLLAYWPWVFG
                     GASVLVILIVTLIGWWALSHVITRLSGVIELQKLDTPAESGQIAPLPVRLHDVTFRYP
                     TAVQDALGPVTLTVEPGEHVAVTGVNGSGKTTLMLVLAGRPPTSGTVERAGAVGLGRV
                     GGTAVIMQHPESQVLGSRVADDVVWGLPPGQSTDIDRLLEEVGLDGLAERDTGGLSGG
                     ELQRLAVAAALAREPSLLIADEVTSMVDQTGRDALLGLLAGLRERHQMALVHITHYNN
                     EADSAERVVNLTGIGITDNTEMVELAAAPTARAPVDARSAAAVIEVDGVCHEYASGTP
                     WATTALRDVSFDVHEGDGLLIHGGNGSGKSTLAWIMAGLTVPTAGSCLLDGRPVSDQV
                     GAVAMSFQAARLQLMRGQVDREVASAAGFSPHDTARVAAALANVGLDASLATRRIDQL
                     SGGQMRRVVLAGLLARSPRALILDEPLAGVDAASQRGLLRLLEDLRRRTGLTVIVISH
                     DFAGLEDLCPHTLELDNGAVQPASATAGGAA"
     gene            8601..9056
                     /locus_tag="VF874_06720"
     CDS             8601..9056
                     /locus_tag="VF874_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9175792.1"
                     /translation="MSPSPSTGASLGVDLLGVVAKLNRLATQRTQLAVPWAQARLLGT
                     IEDQGPTRISNLAALDHCSQPTMTMQVRRLEDAGLVTRTTDPADARAVLIEITPEGVE
                     MLRHVRAARAAVIDPHIAALDRADRQTLSETVHVLQRLLDRAASSPTTD"
     gene            9231..9815
                     /locus_tag="VF874_06725"
     CDS             9231..9815
                     /locus_tag="VF874_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175793.1"
                     /translation="MTVVPKSRIVVGAAALAGLAALGGVGAVLNNDTHSATGATRFDV
                     TEVVTTTSPPDNSALTKLMKMLPAGFDANNCAVVDNPPPDSLATVDCEQNSLPNGPTS
                     GRFSLYPDEATVETHFQTAASEDQILPCPGGPASASNWQYAESPNEPAGKVVCGTYEG
                     TPDLVWSQNDKLLLGNLQGTDVQSIYDFWSNNTI"
     gene            complement(9817..10557)
                     /locus_tag="VF874_06730"
     CDS             complement(9817..10557)
                     /locus_tag="VF874_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX9175794.1"
                     /translation="MTSSEAELPSPGAAAGLPHEFDHTHSDVSGGWLRAASFGAMDGL
                     VSNTALIAGVAASADRHTVVLSGIAGLLAGAFSMALGEYTSVTTANEQIDSEVRLERR
                     AFRMHPQAEREELVQMLVQMGMTEKTARAASTELHQDENKAINFHMVQELGVDPREKP
                     SARLAALSSFVLFAIGAIIPLIPYLLGFASLAAGLACGGVGLVVAGGVSARFTGKPVW
                     WASGRQLAFGAIAIAATYVVGLLIGATA"
     gene            10657..>11777
                     /locus_tag="VF874_06735"
     CDS             10657..>11777
                     /locus_tag="VF874_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9175795.1"
                     /translation="MASTTAQISPDLARHLCNALDGRWHMVKGRSREQLSGEVFRPHY
                     TPNTVIARTKVLDQLKIIAAAGSAEDGFRKEHGGTGDVGAAVTTIEMLAMSDLSLMVK
                     AGVQWGLFGGAIENLGTERHHEAYVRKIIDLELLGCFAMTETGHGSDVQSLETTATYD
                     RETDEFVINSPTKTSRKDYIGGAAETATMAAVFAQLITAGPHEEPTNHGVHCFVVPIR
                     DEEGNDLPGVTTSDCDYKGGLPGVDNGRIVFDNVRVPRDNLLNKYGDVADDGTYSSPI
                     DNPNRRFFTMLGTLVRGRVTVGGSAAAAARVALDIATRYALLRKQFKAPDDDSEVLLM
                     DYLVHQRRVLPLIAKSYALQFAQNELVAKLDEVQSTEIP"
BASE COUNT         2061 a         4201 c         3681 g         1834 t
ORIGIN      
        1 aataccgcag cagataccgc agtgcttcca gacttcacca gcgccagcca acgcaataag
       61 catcatcgcc gcacgtagag gcaggatacc gatatcgcac aagcgccagc caacgtagca
      121 aacgcgcaga acgacggatt atgagtccgc ggctctaacc aactgagcta ccgccccctg
      181 cgaatcacca tcgtaggtgc gccgtcactg atactgcgtc tggccgtcgg cgaggatgcg
      241 ataccgctcg gtgccgctct cgatccgggg cgcgtctgct gccatcgcga cggcaatctt
      301 gttggcagcg ttgcgcatca cgtcgaacgt gatctcgact gcctccgcgt ccgagaagca
      361 agcccggacc tctgccgcga tcgacggcgc gatgtccgcc ggtgaccaga tcagcgcgtc
      421 gacgtaccgc agtgccgcct tgtgcgcgtc ggtcagccga ttcgatgcct cgtacagcga
      481 aatgtcgccg tacaagtcct ccgaaccgcc cgcctccaag gccgtggcct cccgcagcga
      541 attgcacagc cggcagtgat gctgccccgc gccgcgcaac cgcacgatct ccgacgtcac
      601 cggatccagc gccgtctccc tggcgacagc cggcaggaaa gcgtttaaca ctgcatccgc
      661 cgggtccgtc gtgtgatccc aatcagtggg tcccgtcgga acgaccccgc cgagtccgag
      721 cactcgcagt cccgccaaca cccgcggcac gaaatccgcg atgtacgtca gcaccaccgc
      781 accgaacgcg tcaccaccca gcgtcgacag caaccacgcc cgctggtcgt cggaaatccc
      841 cgacacgtcg acactgaact gctcggcgaa ctcgacgacc gcaggctcgg aggcgggctc
      901 gtccggggcg ggtagcggcg gcaatctcag taccgaagcg cacgtctgcc ggaccagcgc
      961 gttgatccgc cccaacgacc cgtccggcga cagcgcgacc agtcgcgaca gttcagcgat
     1021 cacacgccag acgctactgc gcctggctca cgctggacat atagaagtct ggaatcatca
     1081 acggcggcat cgcggcccgc gtcgcccaat caccccactc gcgcggcagc gtgacctccg
     1141 tcgcgcccac ctcggtagcc cgccccagca ggtcgagcgg gctctcgttg aagcggaagt
     1201 tgttcaccgc cgccgacacc ttgccgtcct cgaccaggta caccccgtcg cgcgtcaacc
     1261 cagtcagcag caggttcgtc gggtcgacgg tccggatgta ccacaacgtc gtcagcagca
     1321 gcgcccgctc ggtctggccg atcatgtcct ccaggctcgc gtccccgccc gtcataagca
     1381 ggttgtcggc aggcaccgcg accggcgccc cgaactcggc cgccgccgcc cgcggatacg
     1441 ccagcgcgtt gatcaccccg tcgcgaatcc agtcgacgcg gtcgacgtcc atgccgttgt
     1501 cgaacaccga caccgtgtcc gacgagctgg acgtcgccac gaacggccgg cactgcagag
     1561 gcgcgacgaa aggatccgaa tacagcgtca ccggtagctg ggtcagccgc tcgcccacgc
     1621 gcgtcccgcc gcctggcgcg gagaacgcgg tcctgccctc ctgcgcgccg cggccgtcca
     1681 tcatccacgc catgtagatc atcatgtcgg cgaccgtcga cggcggcatc agcgtcggat
     1741 accggcccgc cggcagcgac agcgtccgcc gcgaccaccc cagccgcgtc gacaggtccg
     1801 acagcagccg atcaacaggc acgtccagga agtcgggcgt gctcacaccg gcccacgcgc
     1861 tcgcaccgtc gcgtttcgcg ttgatctcca ccgacccggt cggctgcgtg aaccgccggc
     1921 gcaccccggt cgacgacgcg acgaacgtcg tcgccatctc gtggcgggcg tagccgtaca
     1981 acgtgtccgg gccgcggaac gcccgcgcaa gtgacgcggc cagcgcaccg aacgcttccg
     2041 caccggtagc cgcgaccgcg agatcccagt cgcccggctc gtccgatccc gtgatcagcg
     2101 gcgcgctgtc gcgggcctcc ggcgcggcac gggccgcgtc ttcgctcgcg gcgaccacgt
     2161 cgcggatcac cgccgggtcg acctcgctgg tctgcaccga cccgacgtgc gccttgtcga
     2221 agtcgcggac gaccgagatc accgacatcg accggctcga cgacaccccg ttggtggtca
     2281 tcgagttgtt cgcccaccgc agcgacgctt ccgcgcggtc cgtgacgatc acaatcgtct
     2341 cgctgacctt cgccgccgcc agcgcctcgt cgacgacctg ctgcggcgcg atcaatgccc
     2401 cgcctcggtc atcgtgttca acacccgcac tccgcggaac agtgccgacg gacagccgtg
     2461 gctgaccgcg gccacctgcc ccggctgcgc cttgccgcag ttgaacgcgc caccgagccg
     2521 ccatgtcgac tccccgccga ccgcctcgag tgacccccag aagtcggtgg tcgtggcctg
     2581 gtaggcgacg tcgcgcacct gcccgtcgag ttccccgttg cggatcgacc agaaccgttg
     2641 cccggtgaac tgaaagttgt agcgctgcat gtcaattgac cacgacttgt cgccgacgat
     2701 gtagaggccg tcttccaccc gcgcgatcaa atcctcggtc gaggtgtcct cgtggcccgg
     2761 ctgcagcgac acgttggcca tccgctgaat cggcacgtgg tgcggcgaat ccgcgtagct
     2821 gcaaccgttg gaccgctcct cccccagtcg cggcgcgaac acccggtcca gttgatagcc
     2881 gacgaacacg ccgtcgcgca ccaggtccca gttctgcgcg gccaccccct cgtcgtcgta
     2941 accgacggtg gccaacccgt agtcgacgca ccggtccgcg gtcacgttca tcaccggcga
     3001 gccgtagtgc agcgtgccga gcttgtccgg ggtggcgaac gacgtgccgg catacgccgc
     3061 ctcgtagccg atcgcgcgat cgtattcggt ggcgtggcca atggattcgt ggatcgtcag
     3121 ccacaggttc gacgggtcga tcaccaggtc cgtcggcctc ggctgcacgg tcggcgcctt
     3181 gaccttctcg gtcagcagcg acggaatctc ggcgagttcg tccgaccagt tccagattcc
     3241 gtcgtcgacg acggcttccc acccgcgcgc ggtgggtggt gcgagcgtgc gcatcgagtc
     3301 gaagctgccc gcggcggcgt cgacggccac ggcctcgagc gttggctgca cccgcacccg
     3361 ctgctgagtg atcgacgacc cgtagacgtc ggcgtagaag gtctgctctt tgacgccgtg
     3421 aaagccggca gacacgtggt cgacgccgtc ggccgacagc agccggtccg agtactcctg
     3481 cagcatttcg atcttgtcgg cggccgccac gtcgaacggg tcggtctgat acgacgacac
     3541 ccattcggca tcgcagtaca cgggttcctc agcgagtcgg acgtcctcgg cgttcaacgc
     3601 ccgcagcgtg cgcgccacct ggaccgcccg gcgcgcggtc tcggctgcca cctcgggggc
     3661 caactcggcg tgcgacgcga aaccccacgt gccgtcgacg atcacccgaa ccgccagccc
     3721 cacctcgcgg ctgatcaccg atgtctccaa catcgcgtcg cgcaaatgga tcacctcggt
     3781 gacgatccgg tggatgcgca ggtcagcatg gctggcgccg gcctcggtgg ccgcgctcaa
     3841 cgcggcgtct gccagctcac gacgcggcag ccccaggaag tccgcatcga tccctcgcgc
     3901 cgccacgggt cccaccgtag cggttgggct tgtgcgccga gagcttgccc acgagttctg
     3961 caccagggct gtgaatctcg ccaaagcacg accatggcgc agatttcggc gaggatttac
     4021 atcgcgaacg cgaggaacac agcatcggtg gccggactac tatctcgcat gccccgaccg
     4081 cgtgcccgca ccggcctgct ggcgtacacg ctgctggcgc cgagcttgtt cggggtcacg
     4141 gtgttcttgc tgctgccgat gtttgtggtg gcatggctga gcctgcaccg ctgggatctg
     4201 ctgggaccaa tccgctatgt cggactggac aattggcgct cggtgctgac cgacggcacg
     4261 ttcggcaact cgctgttggt gactgcgctg ttcgtcgtgc tggtgttgcc ggtgcagacc
     4321 gcgctcggac tgtgtgcggc atcgctgctc gcccggcaac tcccgggcag cgcgttcttc
     4381 cggacggtgt acgtgttgcc gtgggtctgt gcgcctatcg cggtgctgtg gcgctggtgc
     4441 ggctgatcaa cgtcgacaaa gtcgcaccgc cggcctccga caccaaccac aacggcgact
     4501 tctcacgcaa catgttcctg cagggacgca tggcgctgtt ccagtccggc acctacaacc
     4561 tggcgcagat cgcggcgaac gcgccgttcc ggtggggcat cgccatgctg cccgccggac
     4621 cggccggcag ggtcagcgtc accaacggga ttgccgctgc gggcaattca gcgtcccggc
     4681 atccggatgc ggtgcgcgcg gtcctcacgt ggatgggcag tgccgaggga aacgcgtatc
     4741 tcggggccaa cggcgccgcg gtgccggccg tgctcgccgc gcagcgcacc tacttcgact
     4801 tctgggcggc gaagggtggg gacgtcgcgc cgttcttcga cctgctgcgc ggtccgcgaa
     4861 ttcctacacc gggcggagcg gggttcgcgg ccggattcga cgcgctgaag ccatatttcg
     4921 acgaaatgtt cctcggccgc ctcgacgtgg cccaggccct agcgcaagcg caagccgccg
     4981 ctaacgccgc ggccgcacgc tagtagtacg tggccgccag cggcactacc gacgcgatcg
     5041 catcgaagtc agcgtcgcgg tgcacaacct ccgcttcgtg tcggattgct atcgccgcga
     5101 taagacaatc gttgaggctg cggatcttcc ggcctcctcg acgcgcggct cgatagatgg
     5161 tggcagctgc tcgaaagtcc accgcaggtt ccacggtgag cgacggtaag ccattgacca
     5221 gccgctcaag cttcccatcg gtgtcgtcac cggcccccgc cagaatttcc attgcgacgg
     5281 gctcacacat cgcgacatcc tctgcgcgct tggccagcag aatgcgcaca ttgaccgccg
     5341 ccggcgaccc agttgcgcgg aagtattcga cccacgccga ggtatcgatg aggatcacgc
     5401 aaggtccgcc ggcggctcgc ttctgatctc gtcgaggtcg ccattccagc ccacgccttc
     5461 aaggctgagg agaaactcct tcgtaagagg cgtgccgacc agacgtcgca atgcaaggtc
     5521 gacggcttcc cgcttcgtcg acaccccgaa ccggcgcatc acctcgtcga ccagccgatc
     5581 gtcgagttcg atgttggtcc gcgacataca ccaagaatac acatctcata caccagcgcc
     5641 gccgaacgtt agaaccgcgt cgagcccagc agcagctcac tggcgatagc ggccgccgac
     5701 accgccgcga cgatcagcag cgtccacgca tcggcccatc ccggccgtga cggtgctgcc
     5761 gagatctgac cggtgccgcc gcgcgcggcg atcgcgtcgc ccatctcgtc ggcgcggcgc
     5821 agtgcgaccg tgatggcggc cgtgaccatg tcgatcagtt cggcccgcca ccaccggcgg
     5881 cgcgcgcgtc gcgtggtcag cacgggacgc ggccgcagcc gacgggccgc gtagaggaca
     5941 cggaattcgt cgatcaacat cgggaacgcc cgcagggcca acccaagcgc agccgcccat
     6001 tcgtcgaccg ggatccgcag ccaccgcagt ggccggccga gcgtggcgac cgccggcgcc
     6061 acgtcggcga catttgtggt ccacgagacc atcccgccga ttccgagcag cacgatcgac
     6121 agcgcggtga tccgcaggaa gtacagcagt ccgcccactc ctagtgtggc agagccgatg
     6181 tggatgtccg gcggcccgcc gccgagcgcc gctgtgacac ctccgagcgc gacgatgatc
     6241 cacaacaaga gcgggaccgt cggcaacgcg ctgcccggaa tccgggcgat ccacgccgcg
     6301 gtcagcacca gcaccgccac caggccgatc ggcacccagc cggggtagaa cgtgagcagc
     6361 acggaaatgc cgaacgccgc aatgagtttc gttccggccc acaattcgtg gatcaaggat
     6421 gtaccgggaa tgggacgcag cagcacaacg ggtcgccgtt gggtcggcgt gctcatgccg
     6481 caccccctgc cgtcgccgac gcgggctgaa ccgccccgtt atcgagctcc agcgtgtgcg
     6541 ggcacaagtc ctccaggccc gcgaagtcgt gcgagatcac gatcaccgtc aggccggtgc
     6601 gacgccgtag gtcctcgagc agccgcaaca gccctcgctg gctggcggcg tcgacgccgg
     6661 cgagcggctc gtcgaggatc agcgcgcgcg gtgagcgcgc cagcagccca gccagcacca
     6721 cgcgccgcat ctgaccgccg ctgagctggt cgatgcgccg ggtggccagt gacgcatcca
     6781 ggccgacgtt ggccagcgcg gccgccacgc gcgctgtgtc gtgcggcgag aacccggccg
     6841 ccgacgccac ctcacgatca acctggccac gcatcagctg aaggcgcgcc gcctggaacg
     6901 acatcgccac cgcacccacc tgatcgctga ccggtctgcc gtcgagcagg caggacccgg
     6961 cggtcggcac cgtcaggccg gccatgatcc acgcgagggt ggacttgccc gaaccgttgc
     7021 cgccgtggat cagtagcccg tcaccctcgt ggacgtcaaa gctgacgtcg cgcaaagccg
     7081 ttgtggccca aggtgttccg ctggcatact cgtgacagac gccgtccacc tcgatcaccg
     7141 ccgcggcgga gcgagcgtcg accggcgcgc gtgctgtcgg cgccgcggcc agctccacca
     7201 tctcggtgtt gtcggtgatg ccgatgccgg tcaggttcac cacccgctct gccgaatcgg
     7261 cctcgttgtt gtagtgggtg atgtgtacca gcgccatctg gtgacgctcc cgcagcccag
     7321 cgagcagccc aagcagcgcg tcccggccgg tctggtcgac catgctcgtc acctcgtcgg
     7381 cgatcagcag cgacggctcc cgcgcaagcg cggcggccac cgccagccgc tgcagctcgc
     7441 cgccggacag gccgccggtg tcccgctcgg cgagcccgtc cagcccgacc tcctcgagaa
     7501 gcctgtcgat gtcggtgctc tggccgggcg gcaatcccca caccacgtcg tcggcgacgc
     7561 ggctgccgag cacctgactc tccgggtgct gcatgatcac cgcggtgccg ccgaccctgc
     7621 cgaggccgac ggcgcccgcc cgctcgaccg ttcccgaggt cggcggccgc ccggcgagga
     7681 cgagcatcag cgtcgtcttc ccggagccgt tcaccccggt gaccgcgacg tgctcaccag
     7741 gttcgacggt cagggtgacc ggcccgagcg cgtcctgcac ggcggtcggg taccggaatg
     7801 tgacgtcatg caaccgcacc gggagcgggg cgatctgacc gctttcggcg ggggtgtcga
     7861 gcttctggag ttcgatgacc cccgacaacc gggtgatcac gtgcgacaag gcccaccatc
     7921 cgatcagggt gacgatcagg ataaccagga ccgacgcgcc gccgaacacc catggccagt
     7981 acgccagcaa cgtggcgaga ccacgcttga gaaagtcggc gacggggtcc atgtacggga
     8041 gatgcgacaa gaaccgtgcc agaccgttca cgttggcggt catcgactcg aacatcaggt
     8101 tgcgcaaccg ggacagcacc gagagcgcga cgacggtgat cacgccgaac gccaggccgg
     8161 cgaccagcgc cacgacgatg atggtgggag tgccgcgccg cttgcgcttg accagcccga
     8221 tcaacccgcc gatgtaggcg cattcgacca cggtcatcat gccgcccatc ccggcgacca
     8281 gaaacgcgat caacccgccg gcgatcgtgg ccgcgatcag caccctaaag cgaaagcggt
     8341 aggccaaaag ccccatcggg acggtgccca gcaacgacaa cccggcagcg aacgggatga
     8401 caaccgcgac gatcgcagtg gccgccgaca gcgccgccat caccgacgcc tgagcgagtt
     8461 ctccgggtcg aagcgccccg cgccctcgct ggctcacgcg ctccggccca tccgcacttc
     8521 ttgattctgc cagccagccg cagtgttctg tgggcagcaa cacgttgatc gtgcatagac
     8581 aacctatgta atttgatggt atgtcccctt cgccgtccac cggcgcgtca cttggcgtcg
     8641 acctgctcgg tgtggtcgcc aaactgaacc ggctggcgac tcagcgcacc cagctagcag
     8701 tgccgtgggc gcaggcacgg ctgctcggga cgatcgaaga ccaggggccc acccggatct
     8761 ccaatctcgc cgccctggac cactgctctc agccgacgat gaccatgcag gtgaggcgcc
     8821 tcgaagatgc cggcctcgtc acccgaacca ccgacccggc agacgcgcgg gcggtcctga
     8881 tcgagatcac ccccgagggc gtcgagatgt tgcgccacgt gcgtgccgct cgcgcggccg
     8941 tcatcgaccc gcacatcgcc gcgctcgacc gcgctgaccg gcagacgctg tccgaaaccg
     9001 tccacgtgtt gcagcgcctg ctcgaccgcg cggcgtcctc accgacaaca gactgacgaa
     9061 ttcgactgag ttgccgcatt aatgctgctg ggggtggacg tccctgaatg agcaaccgaa
     9121 agcagtgcgg cacttgctgt tcgacgattg cagcgatcaa actggcacat gagagccgaa
     9181 taccgcggtt ttcctgcccg atcgcggttc acggattagt gtcaccagcc atgaccgttg
     9241 tgccaaagtc ccgtatcgtc gtcggcgccg ctgcactcgc agggctcgcc gcacttggcg
     9301 gtgtcggcgc cgtcctgaac aacgacaccc acagtgccac gggcgcaacg agattcgatg
     9361 tcacggaagt cgtcaccacc acgtcgccgc ccgacaactc cgccctcacg aagttgatga
     9421 aaatgcttcc cgccggtttc gacgcgaaca actgcgcagt cgtcgacaac ccgccgcccg
     9481 attcgctcgc cactgttgac tgtgagcaga attcgctccc gaatggcccg acgtcagggc
     9541 gattctccct gtatccggat gaagctaccg tcgagacgca tttccagacg gctgccagtg
     9601 aagaccagat cttgccgtgc ccgggtggcc ctgcgtcagc gagcaactgg cagtatgccg
     9661 agtctcccaa cgaacccgcc ggcaaagttg tgtgcggcac ctacgagggc acccccgact
     9721 tggtgtggag ccaaaacgac aaactgctgc tcggcaatct tcagggcacc gacgttcagt
     9781 cgatctacga tttctggtcg aacaacacca tttaactcac gctgtcgcac cgataagcag
     9841 gccgacaacg tacgtcgcgg cgatcgcaat cgccccaaac gccagttggc gacccgatgc
     9901 ccaccacacc ggcttcccgg tgaagcgggc cgagactccc cctgcgacca ccaggccgac
     9961 gccaccgcac gccaatccag cggccaggga ggcaaacccg agcaggtatg ggatcagcgg
    10021 aattatcgct ccgatcgcga acagaacgaa cgacgacaag gcggcaagtc gggccgacgg
    10081 cttctctcgg ggatccactc ccagctcttg aaccatgtgg aagttgatgg ccttgttctc
    10141 gtcctgatgg agctccgtac ttgcggcgcg tgcagttttc tcggtcatgc ccatctggac
    10201 gagcatctgc acgagctctt ctcgctcggc ctgcgggtgc atgcggaacg cacgacgttc
    10261 gaggcggact tccgagtcga tctgctcgtt ggccgtcgtg accgacgtgt actcaccgag
    10321 cgccatcgag aacgcgccgg cgagcagacc ggcgatgccg ctcagcacga cggtgtgcct
    10381 gtcggcgctg gcggccacgc cggcaatcag cgcggtgttg ctgaccagac cgtccatcgc
    10441 cccgaagctc gccgccctca accagccacc ggagacgtcg ctgtgggtgt ggtcgaactc
    10501 atgcggcagc cccgcggcag ccccgggcga cggcaactcg gcttctgacg atgtcatgac
    10561 gacaattcaa ccgctgcagt gtcgcgctga tctattcagg tgaacctacg ttgcgggaca
    10621 ccgtttaacc gacgccgaca caggttaccg tcgggtatgg cgtcgaccac ggctcagatt
    10681 tcccccgacc tcgcccggca tttgtgcaac gctctcgacg gtcggtggca catggtgaag
    10741 ggccggtctc gcgagcagct gtcaggtgag gtgttccgtc cgcactacac acccaacacg
    10801 gtgatcgcgc ggacgaaggt gttggaccag ttgaagatta tcgcggccgc gggctccgcc
    10861 gaagacgggt ttcgcaagga acacggcggc acgggcgacg tcggggcggc ggtcacgacg
    10921 atcgagatgc tcgccatgtc ggacctgtcg ctgatggtca aagccggtgt gcagtggggc
    10981 cttttcggcg gcgcgatcga aaacctcggc accgaacgcc accacgaggc gtacgtgcgc
    11041 aagatcatcg acctcgagct gctgggctgt ttcgcgatga ccgagaccgg ccacggcagc
    11101 gacgtccagt ctctggagac cacggcgacc tacgaccggg agaccgacga gttcgtcatc
    11161 aactctccga cgaagacgtc gcgtaaggac tacatcggcg gcgcagccga aaccgcaacg
    11221 atggcagccg ttttcgcgca gctgatcacg gcgggtcctc acgaggagcc cacgaaccac
    11281 ggggtgcact gtttcgtcgt gccgatccgc gacgaggagg gcaacgacct gccgggcgtc
    11341 accacctccg actgtgacta caagggcggg ctgcccggcg tggacaacgg ccgcatcgtg
    11401 ttcgacaacg tccgcgttcc gcgagacaac ctgctcaaca agtacggcga cgtcgccgac
    11461 gacggcacgt actcgtcgcc gatcgacaac ccgaaccgac gtttctttac gatgctgggc
    11521 acgctcgtcc gcggccgggt gacggtcggc ggcagcgccg ccgcggctgc gcgggtcgcg
    11581 ctggacattg ccactcgata tgcgttgctg cgcaagcagt tcaaggctcc cgacgacgac
    11641 tccgaggtgc tgctgatgga ctacctcgtc caccagcgtc gggtgctgcc gctgatcgcg
    11701 aagtcctacg cgctgcagtt cgcgcagaac gagttagtgg ccaagctcga cgaggtccag
    11761 agcaccgaga tccccga
//
LOCUS       DASWBF010000189        11884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_79055, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000189 DASWBF010000000
VERSION     DASWBF010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11884
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79055"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF874_06740"
     CDS             <1..207
                     /locus_tag="VF874_06740"
                     /inference="COORDINATES: protein motif:HMM:NF019228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX9175796.1"
                     /translation="ETIAATTTSTGLTVRAQLDDAEYPKGIKIPDKQIKALQTQGTLA
                     PHDFHGEWNYTLNPEPRHARDRPG"
     gene            325..666
                     /locus_tag="VF874_06745"
     CDS             325..666
                     /locus_tag="VF874_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9175797.1"
                     /translation="MKPWLLNQDFHDLEFYFAEAVIEVAAIGAGMRWLRMAVVVGVML
                     TGDGFGVQLGTSAARVALEIFIEPQCSHCARLMMFHGGEIADYIYSGDLRVTHRLVTF
                     LDNGQRVLAPR"
     gene            669..938
                     /locus_tag="VF874_06750"
     CDS             669..938
                     /locus_tag="VF874_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175798.1"
                     /translation="MRFSWQANPASDHQYLATIGKMARLAQHVVDRIAAGEPAVDTVA
                     MDNANQKRLNQVLHLVATPTVYDLNTHQVVDTSGNDWLKRLVGHS"
     gene            1070..1843
                     /locus_tag="VF874_06755"
     CDS             1070..1843
                     /locus_tag="VF874_06755"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX9175799.1"
                     /translation="MRRRLIIRLGVALMGVLDRILVRCSRLGDPARFDTADFPWAAEL
                     ERNSALIRREAEEILRDAANVPPLRRISEDHEKIAIDDKWRSFFLWAYGIRVDDNCRR
                     CPQTAALLERIPGLQTALFSILAPGTHIPLHNGATKAILTGHLGLCVPRQRQRCRINV
                     DGKDYAWREGELFVFDDMRMHEVWNDTDEYRVTLMMHVTRPLRFPGSVLADGIIALIR
                     KSPFVQDAHSRLERWTGRSLADPPQLVDVSPLDELRPHQ"
     gene            complement(1799..2287)
                     /locus_tag="VF874_06760"
     CDS             complement(1799..2287)
                     /locus_tag="VF874_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEX9175800.1"
                     /translation="MSRLTTVLNLFRNGRTSDELEATGRKVMRRIKFGIVVAGCLVML
                     QSLLLVAGAWRNDRQIERNMGVAQAEVLSAGPRRSTIEFVTPDRVTYRPELGVLYPSE
                     LADGMRIYVEYDRNDPNLVRVQHRNAALAIIPAGSIAVVGWLVAGALLVGTKFIERRY
                     VH"
     gene            complement(2284..3909)
                     /gene="menD"
                     /locus_tag="VF874_06765"
     CDS             complement(2284..3909)
                     /gene="menD"
                     /locus_tag="VF874_06765"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX9175801.1"
                     /translation="MNPSTAQARVVVDELIRGGVRDVVLCPGSRNAPLAFALRDADVA
                     GRVRLHVRIDERTAGYLAIGLAVAGQAPVPIVMTSGTAVANLGPAVAEANYARVPLIV
                     LSANRPYELLGTGANQTMEQLGYFDTQVRAAISLGLAEEGTEKMADQNARWRSAVCRV
                     LVAATGARSANAGPVQFDIPLREPLVPDPEPDLGAVPPGRPNGRPWTYTPPVTFDQPL
                     DIDLSRNTVVISGHGAGVHPNLAALPTVAEPTAPHAANPLHPLALPLLHPQQVIMLGR
                     PTLHRTVSSLLADPAVPVFALTTGPRWPDVSGNSQATGTRAVTSGTPSDGWLRRCADV
                     NRRAVEAVRGQLDAHPLTTGLHVAAAVADALRPGDQLVLGASNPVRDAALVGLEPQGV
                     KVRSNRGVAGIDGTVSTAIGAALAHEGRTVALIGDLTFVHDASGLLIGPTEPVPQDLT
                     IVVSNDNGGGIFELLEQGDPRFSDVSSRVFGTPHDVDVGALCRAYHIESRQVEVGDLV
                     DAVNTRFSGVRVLEVKADRSTLRQLHASIKAAI"
     gene            complement(3922..4710)
                     /locus_tag="VF874_06770"
     CDS             complement(3922..4710)
                     /locus_tag="VF874_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175802.1"
                     /translation="MVNLAYDDRGSGEPVLFIAGRGGAGRTWHPFQVPAFLAAGYRVV
                     TFDNRGVGATENADGFTTETLVADTAALIEQLGIARTNIVGVSMGAYIAQELMLARPE
                     LVKRAALMATRGREDRTREFFRKSEAELTNSGVQLPAAYDAKVRLLENFSAHTLNDDR
                     AVADWIEMFTIWPTKQTPGLRCQLNIRPLGNRLPAYRSITTPVLVIGFADDVVVPPHL
                     TKEVADALRNGRYLQIAHAGHLGFLERPEAVNAAILQFFADLPL"
     gene            complement(4735..5478)
                     /locus_tag="VF874_06775"
     CDS             complement(4735..5478)
                     /locus_tag="VF874_06775"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX9175803.1"
                     /translation="MQIAIVLYPGFTALDFIGPYEVLRWLPDAEVRFVWHEPGPITAD
                     SGVLIVAATHSFAETPSPDVILVPGGFSTMEHARDETLLDWVRNAHKTATWTASVCSG
                     SIILAASGLLDGKRATSHWAALAALKALGVKPVGDERIVHEGDIVTCAGVSAGIDLGL
                     WLAGQIGGESRAKVIQLSMEYDPQPPFDSGHMSKASASTKAAATALMSKDLVTRGQLK
                     PMTQLVWDHAIKTAGERRGRSRFRRRERV"
     gene            complement(5491..6440)
                     /locus_tag="VF874_06780"
                     /pseudo
     CDS             complement(5491..6440)
                     /locus_tag="VF874_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771206.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
     gene            complement(6509..7465)
                     /locus_tag="VF874_06785"
     CDS             complement(6509..7465)
                     /locus_tag="VF874_06785"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX9175804.1"
                     /translation="MTRPPDLADLLDRVHVVALPMRVRFRGITIREVALIDGPAGWGE
                     FGAFLEYEPPEAAHWLASAIEAAYEKPLVGRRDRIPVNASIPAVPPELVPELLARFPG
                     ARTAKVKVAEPGQTLHDDVTRVEAVRALVPTVRVDANGGWTVEEAVAAAQALGPLEYI
                     EQPCKTVEELAEVRRRIDIRVAADESIRKADDPLHVVRAGAADVAVLKVAPLGGAAVL
                     LKIAEQIDIPVVISSALDSAVGIATGLQAAAALPTLAHACGLATGRLFVEDVAESAQL
                     VDGCLPVGPVAPDPARLEELRATAERRDWWIRRIIACYRLLC"
     gene            complement(7462..9030)
                     /locus_tag="VF874_06790"
     CDS             complement(7462..9030)
                     /locus_tag="VF874_06790"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX9175805.1"
                     /translation="MTERPPAPRFLDDRLAHWAKTKPDEEAITDLNRTWTWAEVNDRV
                     RRLAGALAELGIGHGDVVAYLDKNHPAGVELTLAAASLGAANAVINFRLAGDELDYVI
                     NDCGAKVLIVGSELKPAIDKIRDRLSNVERVIEVTPQGGDGDEYEALLASGTPTGRAD
                     DVDPDDVCIIMYSSGTTGRPKGVALTQKNVLAHTFNAFADWELEPGDKNLVAMPLFHV
                     AGSCYAQYGVQEGYPIVMTREPDGASLAGGIMRGANRTFLVPAVLAKVLQSGDDAVKL
                     FSALKTYAYGASPMPLPLVRVALDAWPNTDFIQVYGLTEVCGAVTHLMPDAHRDPDHP
                     ERLVSAGTLIKDVELRVVDPDTLDDVPAGELGELWFRTPQLMKGYHNKPEATAEAVTD
                     DGWFRTGDLGRVDEDGFIFIEDRLKDMIISGGENIYSIEVERVLGEHPAVTDVAIIGV
                     PDDKWGEVVKAVVELEGDATEEELIAWCRERLASYKCPKTVDVKGELPRNPTGKILKK
                     DLRKQYWEGRDRAV"
     gene            9209..9922
                     /locus_tag="VF874_06795"
     CDS             9209..9922
                     /locus_tag="VF874_06795"
                     /inference="COORDINATES: protein motif:HMM:NF025398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX9175806.1"
                     /translation="MLLIAAAVTVVGLTSACSTTVDGTPKAERSQPPAPATGTPAAPA
                     ATGVGEQQLDGLLLTLDDVRAIMAAPELEVHDNYAQMPPSTAGYVPEDCVRAAFNTVE
                     PGYRDSKFVAIRGVVMQEPDSSAELSHIVDQGVVTFPDEAAANTYVTRTIEAWRRCAG
                     TPFTVLRPEAAEHWTFGDVTESNGISAIPKTGDGSEWSCSHAITARANAVVDVSACGF
                     SITDQATTIAGRIRDKFPA"
     gene            9956..11590
                     /gene="fadD8"
                     /locus_tag="VF874_06800"
     CDS             9956..11590
                     /gene="fadD8"
                     /locus_tag="VF874_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD8"
                     /protein_id="HEX9175807.1"
                     /translation="MTPVVASSRERSSGELLRHPIHSGHLLAGALKRHKDKPLLFLGD
                     TTLTGSQLADRITQYIQAFEALGAGTGAAVGLLSLNRPEVLMIVGAGQTQGYRRTALH
                     PLGSLDDHAYVLSDAGVTSLIIDPNPMFTERALGLLERVGSLKQILTIGPVPDALAEV
                     AVDLSAEAAKYSPRALVAAEIPLDQIVGLTYTGGTTGKPKGVIMTAQTTSTMTSIQLS
                     EWEWPENPRFLMITPLSHAGAAFFMPTVVKGGEMIVLSKFDPAEVLRVIEEQRITATM
                     LVPSMLYALMDHPDSRTRDLSSLETVYYGASAINPVRLKEAIDRFGPIFAQYYGQSEA
                     PMVITYLAKGEHDEKRLTSCGRPTLFARTALLGEDGKPVPQGEVGEICVSGPLLSGGY
                     WKLPDATAETFRDGWMHTGDLAREDEDGFWFIVDRVKDMIVTGGFNVYPREVEDVVAE
                     HQAVAQVCVIGTPDEKWGEAVTAVVVLREDAPRDQESVTAMTVEIQAAVKERKGSVQS
                     PKQVVVVDALPLTGLGKPDKTAVRAKFWEGAGRAVG"
     gene            11723..>11884
                     /locus_tag="VF874_06805"
     CDS             11723..>11884
                     /locus_tag="VF874_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9175808.1"
                     /translation="MVAAVLFDFSGTLFRLEEGDSWFAGMQLDHDAEVDGHVQAELMR
                     RLTAPTGRSV"
BASE COUNT         1938 a         4004 c         4029 g         1913 t
ORIGIN      
        1 gagaccatcg ccgcgaccac caccagcacc ggactcaccg tccgcgccca actcgacgac
       61 gccgagtacc ccaagggcat caagatcccc gacaagcaga tcaaagccct tcaaacacag
      121 ggcacccttg cacctcacga cttccacggc gagtggaact acaccctcaa ccccgaaccg
      181 cgacacgccc gagaccgacc aggttaattc atcacaggcc cttagtgcga cgaagtcggg
      241 cggcgccacc ggttgcgctg acgccggagg cgcgagcata gcggcgagca aggccgtggc
      301 gacgagcccg atactgcgcc gacagtgaag ccatggctgc tgaatcagga ttttcacgac
      361 ctggaattct atttcgcgga agcggtgatc gaagtcgcgg cgataggggc gggtatgcgc
      421 tggctgagga tggccgtggt ggtcggcgtc atgctcaccg gcgacgggtt cggcgttcag
      481 ctcggcacgt cggcggcacg cgttgcgctc gagatcttca tcgaaccgca gtgctcgcac
      541 tgtgcgaggt tgatgatgtt tcacggcgga gagatcgccg actacatcta cagcggcgac
      601 ctgcgggtga cccaccggct ggtgaccttc ctcgacaacg gccagcgggt actcgcaccg
      661 cgttagcaat gcgattttcc tggcaggcca atccggccag cgaccaccag tacctcgcca
      721 ccatcggcaa aatggcacgg ctggcccagc atgtcgtcga ccgcatcgcc gccggcgagc
      781 ccgccgtcga cacggttgcg atggacaacg caaaccagaa gcggctcaac caggttctgc
      841 acctcgtcgc gaccccaacg gtgtatgacc tcaacacaca ccaggtcgtc gacacctcgg
      901 gcaatgactg gctgaagcga ctggtcggcc acagctagcg gcacgttgcg gtgttgcacc
      961 cgaaccagat tcgggttatt cggccgagtt ttggacgtag atacgggttt cgcgtgttaa
     1021 tgtcggcgcg ctcttcacgg aaggcgagcg acacttggag tttcgtggcg tgcggcgccg
     1081 gctgatcatc cgactgggcg tcgcgttgat gggagtgctg gaccggattc tggtccgctg
     1141 ctcacgactc ggcgatcccg cgcgcttcga cactgccgac tttccgtggg ccgccgagct
     1201 ggaacgcaac tcggctctga tccgccgcga ggcagaagaa atcctccgtg atgcggcgaa
     1261 tgtgccgccc ctgcgccgca tctccgagga ccacgagaag atcgcgatcg atgacaagtg
     1321 gcgatcgttc tttctctggg cttacggcat ccgcgtcgac gacaactgcc gccgctgtcc
     1381 gcagacagcg gccctgctag agcgcatccc tgggctgcag acggcactgt tctcgatcct
     1441 ggctccgggc acgcacatcc cgctgcataa cggcgccacc aaagccatct tgaccggcca
     1501 ccttggcctg tgcgtgcctc ggcaacggca gcgttgccgc atcaacgtgg acggcaagga
     1561 ttacgcgtgg cgcgagggcg agctcttcgt gttcgacgac atgcgtatgc acgaggtctg
     1621 gaatgacacc gatgagtacc gcgtcacgct gatgatgcac gtgacgcggc cgctgcgctt
     1681 cccgggctcc gtcctcgcgg acggcatcat cgccttgatc cggaagagtc cctttgtcca
     1741 ggacgctcac tcgcgcctcg agcggtggac gggccggagc ctggccgatc cgccacaact
     1801 agtggacgta tcgcctctcg atgaacttcg tccccaccag tagcgcgccc gcgacaagcc
     1861 agcccaccac cgcgatcgag cccgccggga tgatcgccaa cgcggcgttg cggtgttgca
     1921 cccgaacaag gttcggatcg ttgcggtcgt attcgacgta gatccgcatc ccgtccgcca
     1981 gctccgacgg gtacagcact ccgagctccg gccggtaggt gacgcggtcc ggcgtgacga
     2041 actcgatcgt cgaacgccgc ggcccggcgc tgagcacctc ggcctgcgcc acgcccatgt
     2101 tccgctcgat ctgccggtcg ttgcgccacg caccggcgac cagcagcaac gactgcagca
     2161 tcaccagaca ccccgccacc acaatgccga acttgatgcg gcgcatgact tttcggccgg
     2221 tagcctcgag ttcgtcggaa gtcctgccgt ttcggaagag gttcagcacg gtggtcaacc
     2281 ggctcatatc gccgccttga tcgacgcgtg cagctgtcgc agcgtcgacc ggtcggcttt
     2341 gacctcgagc actcgcaccc cggagaagcg cgtgttgacg gcgtcgacga ggtcgccgac
     2401 ttccacctgc cgggactcga tgtggtacgc ccggcacagc gcgccgacgt cgacgtcgtg
     2461 cggggtgccg aagacccgcg acgacacgtc ggagaaccgg gggtcgccct gctcgagcag
     2521 ctcgaaaata ccgccgccgt tgtcgttgga caccacgatc gtcaagtcct gcggcaccgg
     2581 ctccgtcgga ccgatcaaca gcccggacgc gtcgtgcacg aacgtcaggt caccgatcaa
     2641 cgcgacggtc cggccctcgt gggccaacgc cgcgccgatg gccgtcgaca cggtgccgtc
     2701 gatgccggcg accccgcggt tggaccgcac cttgacgccc tgcggttcga ggccgaccag
     2761 cgcggcgtcg cgcaccgggt tggacgcgcc gagcaccagc tgatcacccg gccgcagcgc
     2821 gtccgccacc gccgccgcga catgcaggcc ggtggtcaat gggtgggcat cgagctgacc
     2881 gcgtacggcc tcgaccgcgc ggcggttcac gtcggcgcag cggcgcagcc agccgtcgct
     2941 cggtgtgccg gacgtgaccg cccgggtgcc ggtggcctgc gagttgccgg agacgtccgg
     3001 ccagcgcggg ccggtggtca gcgcgaacac cggcaccgcc gggtcggcca gcagcgacga
     3061 caccgtccgg tgcagcgtcg gccggcccag catgatcacc tgctgcgggt gcagcagcgg
     3121 caacgcgagc gggtgcagcg ggttcgcggc gtgcggcgcg gtgggttcgg cgactgtggg
     3181 cagcgccgcc aggttcgggt gcacaccggc gccgtggccg ctgatcacga cggtgttgcg
     3241 ggacaggtcg atgtccagcg gctgatcgaa cgtgacgggc ggcgtgtacg tccacggccg
     3301 cccgttcggc cgtccgggcg gcacggcgcc cagatcgggt tcggggtcgg gcaccagcgg
     3361 ctcgcgcagc gggatgtcga actgcaccgg cccggcgttc gcagaccgcg cgcccgtcgc
     3421 cgccaccaaa acccggcaca ccgccgaacg ccagcgcgcg ttctggtcgg ccattttctc
     3481 ggtgccctcc tcggccaggc cgaggctgat cgccgcgcgc acctgcgtgt cgaagtagcc
     3541 gagctgctcc atcgtctggt tggcgccggt gccgagcagc tcgtagggcc ggttggccga
     3601 cagcacgatc agcggcaccc gcgcgtagtt ggcctccgcc accgcgggcc cgaggttggc
     3661 gaccgcggtg ccagacgtca tcacgatcgg gaccggagcc tgcccggcga ccgcgaggcc
     3721 gatggccagg tacccggcgg tgcgctcgtc gatgcgcacg tgcaggcgca cgcggccggc
     3781 cacgtcggcg tcacgcagtg cgaacgccag cggcgcgtta cgcgacccgg ggcacagcac
     3841 cacgtcgcgc acgccgccgc ggatcagctc gtcgacgacg acgcgggcct gcgccgtgga
     3901 gggattcaca cctaaagact gtcacaacgg caggtcggcg aagaattgca ggattgcggc
     3961 gttgacggcc tcagggcgtt cgaggaagcc caaatgaccg gcgtgggcga tctgcaggta
     4021 gcggccgttg cgcagcgcgt cggcgacctc cttggtcaaa tgcggcggca cgacgacgtc
     4081 gtcggcgaac ccgatcacca gcacgggtgt ggtgatgctc cggtaggcgg gcagccggtt
     4141 gcccagtggc cggatgttca gctggcagcg caggccgggc gtctgcttgg tcggccagat
     4201 cgtgaacatc tcgatccagt cggcgacagc gcggtcgtcg ttgagggtat gggccgagaa
     4261 gttctccagc aaccgcactt tcgcgtcgta cgcggcgggc aactgaacac ccgagttggt
     4321 cagctccgct tcggattttc ggaagaactc ccgggtgcgg tcctcacgtc cgcgggtggc
     4381 catcagcgcg gcccgcttca cgagttcggg ccgggccagc atcagctcct gggcaatgta
     4441 ggcgcccatc gacacgccga cgatattcgt ccgcgcgatg ccgagctgct cgatcagcgc
     4501 ggcggtgtcg gcgaccaggg tctcggtcgt gaagccgtcg gcgttctcgg tcgcgccgac
     4561 tccccggttg tcgaacgtga cgacgcgata gcccgcggcc agaaacgccg gtacctgaaa
     4621 cggatgccag gtgcggccgg cgccgcctcg tccggcgatg aacagcacgg gctcgccgct
     4681 gccgcggtcg tcgtaggcga ggttgaccac gcggccattg tcgcttgggt gcggtcagac
     4741 gcgctcacgg cgtcggaagc gggaccggcc gcgccgctcg ccggcggtct tgatcgcgtg
     4801 gtcccagacc agttgcgtca tcggcttgag ctggcctcga gtgaccaggt ccttggacat
     4861 cagcgcggtg gcggcggctt tcgtgctcgc cgaggccttc gacatgtggc cggagtcgaa
     4921 cggcggctgc gggtcgtatt ccatcgacag ctgaatgact ttggcgcggc tctctccgcc
     4981 gatctggccg gccagccaca gtccaaggtc gatgcccgcc gagacgcccg cgcaggtgac
     5041 gatatcaccc tcgtgaacga tcctctcgtc gccaaccggc ttgacgccca acgctttcag
     5101 cgcggcgagc gccgcccagt gtgacgtggc gcgcttgccg tccagcagcc cggacgcggc
     5161 gagaatgatc gacccggagc acaccgacgc cgtccacgtc gcggtcttgt gtgcgttacg
     5221 cacccagtcg agcagcgtct cgtcgcgggc atgctccatc gtcgagaacc cacccggcac
     5281 caaaatgacg tcgggagaag gtgtttcggc gaacgagtgc gtcgccgcga cgatcaggac
     5341 accggagtcg gcggtgatcg ggccaggctc gtgccagacg aaccgcacct cggcgtccgg
     5401 cagccagcgc agcacctcgt agggcccgat gaagtccagc gcggtgaagc cggggtacag
     5461 cacaatcgcg atctgcattc tattgtcctt tcaggcaaag gtcttgcggt agtggtcagg
     5521 cgacacaccg atacggcgaa taaagttgcg gcgcatggtt tctgcggtgc cgaagccgca
     5581 gcgggcggcg atcttcacga cggtgtcgtc ggtttcctcg agctgtcgac gcgcggcctc
     5641 ggtgcggacg cgttcgacgt aggcgcccgg cgcttcgccg acctcgtcgg tgaagacgcg
     5701 cgtgaaatgc cgcgggctca tggccgcctt gcgggccagt tcagcgacgt tgtgcttggc
     5761 gcctggctgc gcttcgatgg catcctgcac gtcgcggatc gggctacgat gggcgcgcgg
     5821 catccacacc ggagcggcga attgcgtttg gccgccgggc cttcggagat acagcaccat
     5881 ccaacgggcc acggtctgcg cgacatcggt gccgtggtcg tcttcgacga gcgacagtgc
     5941 caggtcgatg cctgcggcca cgcccgcggc ggtccagacc ttcgcggagc tgcgcagaaa
     6001 aatcggttcg ggatcgacat cgacggccgg gaactcgtcc tgcagctgcc gggcgaacgc
     6061 ccagtgcgtg gtggcccggc agccgtcgag caggccggct tcggccgcca ggaatgcacc
     6121 ggtgcagacg ctgacgacgc gccgtgctgc ttgggcggcg gtgtgcagcc aggtcatgac
     6181 gtcggcgttg gcgcgggcgc cgtccacacc gacaccgccg ggcagcagca gggtgtcgat
     6241 gggttcacgc gggtcgggca gtttcgtggc gacgaactcg agccccgtgc ccgtcgccac
     6301 cggctgcccg tccaccgacg ccagggccac ctcataagcg ccgttgacca gcaacgacgc
     6361 gccagtgaac acgtcgaacg gcccgaccaa tccagcgcct ggacgccggg gaagccgaga
     6421 atcaccacct tgcgcgccac ggcatccagt ctcgggcaca acaggctatg tcgtctatgc
     6481 catgtatccc acaaatcagg acacgccatc aacacagcag tcggtagcac gcgatgatgc
     6541 gccggatcca ccagtcgcgt cgctcagcgg tcgctctcag ttcttccaga cgcgccggat
     6601 cgggcgcgac gggtccgacc ggcagacagc cgtcgaccag ctgggccgac tcggcgacgt
     6661 cctcgacgaa cagccgcccg gtcgccaagc cgcacgcgtg cgccaaagtc ggcagcgcgg
     6721 ccgccgcctg caagccggtc gcgatgccga ccgccgaatc aagcgcgctg gagatcacga
     6781 ccggaatgtc aatctgctca gctattttca gcagcaccgc ggcgccgccg agcggagcga
     6841 ccttcagcac ggccacgtcc gcggcgccgg cgcggaccac gtgcagcggg tcgtcggcct
     6901 tgcggatgct ttcgtcagcg gccacccgga tgtcgatgcg ccgacggacc tcggccagct
     6961 cctcgacggt cttgcacggc tgctcgatgt actccagcgg tccgagcgcc tgcgccgcgg
     7021 ccactgcttc ctcgacggtc cacccgccgt tggcgtcgac ccgcaccgtc ggcaccagcg
     7081 cccggaccgc ctcgacgcgc gtgacgtcgt cgtgcagcgt ctgcccgggc tcggcgacct
     7141 tgaccttggc ggtgcgcgcc cccggaaagc gggccagcaa ctcgggcacc agctcgggcg
     7201 gcaccgcggg gatgctcgcg ttgaccggga tgcggtctcg ccggccgacg agcggcttct
     7261 cgtaggccgc ctcgatcgcc gaagccagcc agtgcgccgc ctcgggcggc tcgtactcga
     7321 gaaaggcgcc gaactcgccc cagccggcgg gcccgtcgat cagcgcgacc tcgcgaattg
     7381 tgatgccgcg gaagcgaact cgcatcggca acgcgaccac gtggacgcgg tcgagcaggt
     7441 cggcgaggtc cggtggccgg gtcacaccgc tcggtcgcgg ccttcccaat attgtttgcg
     7501 cagatccttt ttcaggatct tgcccgtcgg gttccggggt agctcgcctt tgacatccac
     7561 ggtcttcggg cacttgtagc tcgccaaccg ctcgcgacac cacgcgatca gctcctcttc
     7621 ggtcgcgtca ccctcgagtt cgacgaccgc cttcacgacc tcgccccact tgtcgtcggg
     7681 cacaccgatg atggcgacgt ccgtgaccgc cggatgttca ccgagcacgc gctcgacctc
     7741 gatcgagtag atgttctcgc cgccggagat gatcatgtcc ttgagccggt cttcgatgaa
     7801 gatgaagccg tcctcgtcga cccggccgag gtcgccggtg cggaaccatc cgtcgtcggt
     7861 gacggcctcg gccgtggcct ccggcttgtt gtggtagccc ttcatcagct gcggtgtgcg
     7921 gaaccacaat tcaccgagtt cgcccgcggg cacgtcgtcg agggtgtccg ggtcgacgac
     7981 acgaagttcg acgtctttga tcagggtgcc ggcgctgacg agccgctcgg ggtggtcggg
     8041 gtcgcggtgg gcgtccggca tcaggtgggt gacggcccca cacacctcgg tgagcccgta
     8101 cacctggatg aaatccgtgt tgggccacgc gtccagcgcc acgcgcacca gcggcagcgg
     8161 catcggcgag gcgccgtatg cgtacgtctt gagcgcgctg aagagcttga ccgcgtcatc
     8221 gcccgactgg agcaccttcg ccaacacggc cggaaccagg aatgttcgat tggcgccgcg
     8281 catgatcccg ccggccaacg aggcgccgtc cggctcgcgc gtcatcacga tcggatagcc
     8341 ctcctgcacc ccgtactggg cgtagcacga gccggccacg tggaacaacg gcatcgcgac
     8401 caagttcttg tcgccgggct ccagctccca gtcggcgaac gcgttgaacg tatgcgcgag
     8461 cacgttcttc tgggtcagcg caacgccttt cggtcgaccg gtggtgccgg acgaatacat
     8521 gatgatgcac acgtcgtcgg ggtctacgtc gtcggctcgg ccggtgggag tgccagaggc
     8581 gagcagtgct tcgtactcgt caccgtcgcc gccctgcggc gtgacctcga tgacgcgttc
     8641 gacgttggac agccggtcgc ggatcttgtc gatggccggt ttcagctcgc tgccgacgat
     8701 caacaccttt gcgccgcagt cgttgatcac gtagtcgagt tcgtcgccgg cgagccggaa
     8761 gttgatgacc gcgttggccg cccccagcga cgcagcggcc agcgtcagct cgacgccggc
     8821 gggatggttc ttgtcgaggt acgcgacgac gtcgccgtgc ccgatcccga gctcggccag
     8881 cgcgccggcc agtcggcgga cccggtcgtt gacctccgcc catgtccagg tgcggttgag
     8941 gtcggtgatg gcttcctcgt caggcttcgt cttcgcccag tgcgccagac ggtcgtcgag
     9001 gaaacgtggt gccggggggc gctcagtcat gcaacgagcg tgccacgaac acctgcttcc
     9061 catccaggaa aatgagcact atccgcagag ctgaagaggg gcgaccgccg aaaaccaccg
     9121 cgtagttagc gacaagtacg gcggttttgc gcgtcggcac cctaaacttc accgaaatac
     9181 tcagggcggg tgaggcgacg tgtggcgagt gctgttgatc gcagcggcgg tgacggtggt
     9241 cggcctgacg agcgcctgct cgaccaccgt ggacgggacc ccgaaggctg agcggtctca
     9301 gcctcctgcg cccgcgaccg gtactccagc cgccccagcg gccaccggcg tcggcgagca
     9361 gcagctggat ggcttgctgc tgactctgga tgacgtccgg gcgatcatgg cggcccctga
     9421 actcgaggtg cacgacaact acgcccagat gccgccgagc accgcgggct acgtgccgga
     9481 agactgcgtg cgggcggcct tcaacaccgt cgagcccggc taccgggaca gcaagttcgt
     9541 cgcgatccgc ggcgtcgtca tgcaggagcc cgactccagt gcggagttgt cgcacatcgt
     9601 cgaccagggc gtggtgacgt tcccggacga ggccgccgcc aacacctatg tcacgaggac
     9661 gatcgaagcg tggcggcgtt gtgcgggaac gcctttcacc gtgttgcggc ccgaagccgc
     9721 cgagcactgg accttcggtg acgtcaccga gagcaacggc atcagcgcca tccccaagac
     9781 gggcgacggc tccgagtggt cgtgttcgca cgcaatcacc gcgagggcca acgcggtggt
     9841 cgacgtgtcc gcgtgcggct tttcgatcac cgaccaggcc acgacgatcg ccggccggat
     9901 ccgggacaag ttccccgcgt aggacgaatc tgtaacacgt tctagtcttg agcccatgac
     9961 tccggttgtt gcctcttcgc gcgagcgctc atcgggcgag ttgctgcgcc atcccatcca
    10021 ctctggccac ctgctcgccg gggcgctgaa gcgtcacaag gacaagccgc tgctgttcct
    10081 cggcgacacc acgctgaccg gcagccagct ggccgaccgc atcacgcagt acatacaggc
    10141 cttcgaggcc ctgggcgccg gcaccggcgc agcggtcggg ctgctgtcgc tgaaccggcc
    10201 cgaggtgctg atgatcgtcg gcgccggcca gacccaggga tatcgccgca cggcactaca
    10261 ccctctcggc tccctcgacg accacgcgta cgtgctgtcg gacgcgggcg tcacgtcgtt
    10321 gatcatcgac ccgaacccga tgttcaccga acgggctctg ggtttgctgg agcgtgtcgg
    10381 gtctctgaag cagatcctga cgatcggccc ggtgcccgac gcgctcgccg aggtcgcggt
    10441 ggacctgagc gccgaagccg ccaagtactc gccccgggcg ctggtcgctg ccgagattcc
    10501 gctcgatcag atcgtcggcc tgacctatac gggcggaacg accggcaagc ccaagggcgt
    10561 catcatgacc gcgcagacca cctcgaccat gacgtcgatc cagctgtccg agtgggaatg
    10621 gccggagaac ccgcgctttt tgatgatcac cccgctgtcg cacgccggcg ccgcgttctt
    10681 catgccgacg gtggtcaagg gcggcgagat gatcgtgctc tccaagttcg atccggccga
    10741 agtcttgaga gtcattgaag agcaacgcat tacggcgacg atgttggtgc cgtcgatgct
    10801 gtacgccctt atggatcacc cggattcacg gacccgtgac ctgtcgtcgc tggagactgt
    10861 gtactacggc gcgtcggcga tcaacccggt gcggctgaag gaggcgatcg accggttcgg
    10921 gccgatcttc gcgcagtact acgggcagtc cgaggcgccg atggtgatca cgtatctggc
    10981 caagggcgag cacgacgaga agcggctgac gtcgtgcggg cgaccgacgc tgttcgcgcg
    11041 gacggcgttg ttgggtgagg acggcaagcc ggtgccgcag ggcgaggtcg gcgaaatctg
    11101 tgtcagcgga ccgctgttgt cgggcgggta ctggaagttg cccgacgcga cggccgagac
    11161 cttccgcgac ggctggatgc acaccggcga cctggcccgc gaggacgagg acggcttctg
    11221 gttcatcgtc gaccgggtga aggacatgat cgtcaccggc gggttcaacg tgtatccgcg
    11281 cgaggtcgag gacgtggtcg ccgagcatca ggcggtggcg caggtgtgcg tgatcggcac
    11341 gcccgacgag aagtggggcg aggccgtcac cgcggtcgtg gtgctgcgcg aggatgcgcc
    11401 gcgcgaccag gaatctgtga cggcgatgac cgtcgagatc caggctgccg tcaaggagcg
    11461 caaaggatcg gtgcagtcgc cgaagcaggt cgtggtcgtc gacgcgctgc cgctgaccgg
    11521 gctgggcaag ccggacaaga cggcggtgcg cgcgaagttc tgggagggcg cgggccgcgc
    11581 cgtgggctga cgttcaccac gcgttcaccg caggtcaacg agttgtcgct cacgaggcgg
    11641 gcaagaaggt ggttgacgac tcaggcgctc aggtagtcgg cacagtgggt gaggccggga
    11701 tcgccgctgg cacgataggc gcatggtggc ggcggtgttg ttcgacttct ccggcacgct
    11761 gtttcggctc gaggaaggcg acagctggtt cgccgggatg cagctggacc atgacgccga
    11821 ggtggacggg cacgtccagg cggagttgat gcgcaggctg accgccccca ccggccggtc
    11881 ggtc
//
LOCUS       DASWBF010000190         7952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_743359,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000190 DASWBF010000000
VERSION     DASWBF010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7952
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743359"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VF874_06810"
     CDS             <1..529
                     /locus_tag="VF874_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9175809.1"
                     /translation="PPEITDAVRDYSAPSFPAGRPLIDALRDLSSRIYADFTYRSGST
                     TVSTRVAQVLAAREGVCQDFARLAVACLRANGLAASYVSGYLATDPPPGKQRVVGIDA
                     THAWAAVWTPGDQWLAFDPTNDQMVDERYILVGWGRDYADIPPLRGIIYTDSERSAIE
                     VSVDVAPFEGDVVHA"
     gene            522..1583
                     /locus_tag="VF874_06815"
     CDS             522..1583
                     /locus_tag="VF874_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEX9175810.1"
                     /translation="MRDFVCPNCGQHLAFENSVCLSCDRPLGFSLDDMALLVIAEGPD
                     AAKPGFVDAANYELCANLHLAQCNWFVQKEPVRRLCTSCRLTRTRPNDADTTALSAFA
                     DAEKAKRRLIAELHELRLPIVGRDDDPEFGLAFDLLSSEKDKVFTGHHNGVITLDLAE
                     GDDVHREQLRIEMDEPYRTLLGHFRHEIGHYYFYRLVEPSQEYSERFRELFGDPDADY
                     QEALDRHYREGAPPDWEETYVSSYATMHAAEDWAETFAHYLHIRDTLDTAAAFSFAPA
                     NATYERKVLGPSGFNTIIEMWLPLSWGLNMVNRSMGRDDLYPFVLPVPVLDKMRFIHT
                     IIDEVTADPTKLAAATPTS"
     gene            1653..1961
                     /locus_tag="VF874_06820"
     CDS             1653..1961
                     /locus_tag="VF874_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEX9175811.1"
                     /translation="MTPRFGVRFGASVFIGALALQLTGCSKTIKPEGAAKSVTDLVSE
                     QTHFTPNDVKCPSGVDAEVGQEFDCHFTGPDGAYTAHLKVTKVEGDDVEFYITTKLDQ
                     "
     gene            1992..2240
                     /locus_tag="VF874_06825"
     CDS             1992..2240
                     /locus_tag="VF874_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175812.1"
                     /translation="MSRSWFPDVVVHRHEGVACFDSPDQWFFNDIRGWTLADHVDDEQ
                     FSRFRAGALRRCRRPGPVRGAGPDRDGHPPGLTARACA"
     gene            complement(2314..2823)
                     /locus_tag="VF874_06830"
     CDS             complement(2314..2823)
                     /locus_tag="VF874_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175813.1"
                     /translation="MLLNRATADGIAGGTIRIDNVVERPAGYRVTEAQARAAGYRGAA
                     AAHAELDRRSAEHVFVITVSYAGPDERPALAANDALTADDVAVISERLARFDAASGKG
                     PWTRRYLELIRDNEGVRAPDLAAREGLDVPRFKRRVRQLKGLELTISLDVGYRISPRG
                     TTFLRLTRH"
     gene            2865..4241
                     /locus_tag="VF874_06835"
     CDS             2865..4241
                     /locus_tag="VF874_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX9175814.1"
                     /translation="MSDVAGRAAPKSDGLFDLPGTSDSADWQGRGAGLPAPLAVRMRP
                     ATLDEVVGQSHLLTERSPLRRLIDGSGAASVILYGPPGTGKTTLASLISQATGRRFEA
                     LSAVSAGVKEVRAVIENARRAAAHGEQTVLFIDEVHRFSKTQQDALLAAVENRVVLLV
                     AATTENPSFSVVAPLLSRSLILQLKPLSADDIRTLIQRAVDDPRGLGGRVEIEPDAVD
                     LLVRLSAGDARRALTALEVAAETVTDAGGPVTVASIEESLDKAAVRYDRDGDQHYDVI
                     SAFIKSVRGSDVDAALHYLARMLVAGEDPRFVARRLMILASEDIGMADPTALQTAVAA
                     AQTVQLIGMPEAQITLAHATIHLATAPKSNAVVAAIGAAMSDIKAGKTGLVPAHLRDG
                     HYSGAAALGNAIGYRYSHDDPDGVVAQQYPPDELVGTDYYHPTEHGNEREIAKRLNRL
                     RAIIRKRR"
     gene            complement(4242..4556)
                     /locus_tag="VF874_06840"
     CDS             complement(4242..4556)
                     /locus_tag="VF874_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX9175815.1"
                     /translation="MTIHSVLGAILIGLVIGALARFVLPGKQPIPIWLTILVGIGSAF
                     VGTALARAMGIPIDTPGVDWGELLVQVIVAVLGVALVTAAFGRSTFGRRRFGASRLTR
                     RW"
     gene            complement(4621..5250)
                     /locus_tag="VF874_06845"
     CDS             complement(4621..5250)
                     /locus_tag="VF874_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175816.1"
                     /translation="MANARRWLAGAAGAMGGLELYRRAIRPWMYAWGASPDELSAVLP
                     GDELVSAVTPRTTRGVTIDAPVKDVWPWLAQIGEDRGGYSYDWLERSVGTRIHNAQTI
                     NPDWQELRVGDSVWLARRYGPMGRQVVAAVEPKSHLVLMSEADYDRVTRGEKALGSWA
                     FYLIPDGKRTRLLARGSGGAVGTATFDIPHFVMEQKMLRGIRDRAQRGN"
     gene            complement(5429..6049)
                     /locus_tag="VF874_06850"
     CDS             complement(5429..6049)
                     /locus_tag="VF874_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX9175817.1"
                     /translation="MFARSTTIEARPEAIDAGIAHVRDEVMPALEAMHGCIGISLMVD
                     RETGRCITTTAWESEDAMRASAEQVAPVRDRAADILGGTATVQEWEIAVLHRDHRSGE
                     GACVRATWFKVPNGQGGRSVELYKDSVLPALEELEGFCSASLLVDRSSGRAVSSVTFD
                     SADAMDRNREQARSLRTARLRELGADQLDVGEFELAIAHLRVPELI"
     gene            6160..6561
                     /locus_tag="VF874_06855"
     CDS             6160..6561
                     /locus_tag="VF874_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX9175818.1"
                     /translation="MDTAVLDVDTARRRIVDLTDEVRSFCASHGDGLCNVFVPHATAG
                     VAVIETGAGSDDDLVDTLERLLPRDDRYRHAHGSPGHGADHVLPALITPSVTMPVQRG
                     KPLLGTWQSVVLVDLNRDNPRRSVRLSFVDG"
     gene            6634..>7952
                     /gene="alaS"
                     /locus_tag="VF874_06860"
     CDS             6634..>7952
                     /gene="alaS"
                     /locus_tag="VF874_06860"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX9175819.1"
                     /translation="MQTHEIRKRFTDHFVKAGHTEVPSASVILDDPNLLFVNAGMVQF
                     VPYFLGQRTPPHRKATSIQKCIRTPDIDEVGVTTRHNTFFQMAGNFSFGDYFKRGAIE
                     LAWTLLTNPIEDGGYALDPEKLWATVYLDDDEAAGLWQEIAGLPQERIQRRGMADNYW
                     SMGIPGPCGPASEIYYDRGPEYGPEGGPVASEDRYIEIWNLVFMENLRGDGTSKTDFE
                     ILGPLPRKNIDTGMGVERMACVLQGVDNVYETDLLRPVIDKVAEIAPRGYGKGNHDDD
                     VRYRIIADHSRTAAILIGDGVSPGNDGRGYVLRRLLRRIVRAAKLLGIDTPIVGKLMA
                     TVRDAMGPSYPELITDFARITRIAEAEETAFNRTLASGSRLFEEAASATKAAGAKVLS
                     DSDAFTLHDTYGFPIDLTLEMAAEAGLAVDENGFRRLMAEQRERAKA"
BASE COUNT         1354 a         2737 c         2585 g         1276 t
ORIGIN      
        1 tccgccggag atcaccgacg cggtccgcga ctactccgca cccagcttcc ccgcgggacg
       61 cccgctgatc gacgcgctgc gcgacctgtc gtcgcggatc tacgccgact tcacctaccg
      121 gtccgggtcg acgacggtgt cgactcgggt cgcacaggtc ttggccgcgc gtgaaggggt
      181 gtgccaggac ttcgcgcgcc tggccgtcgc ctgcctgcgc gccaacggac ttgcggcaag
      241 ctatgtttcg ggatacctgg ccaccgatcc accgcccggc aagcagcgtg tggtgggcat
      301 cgatgcgacg cacgcgtggg ctgcggtgtg gactcccggt gatcagtggt tggcgttcga
      361 cccgaccaac gaccagatgg tcgacgagcg gtacattctc gtgggctggg gtcgcgacta
      421 cgccgatatc ccgccgctgc gcggcatcat ctacaccgac tccgagcgca gtgcgatcga
      481 ggtgtcggta gacgtggcgc catttgaggg ggacgtggtt catgcgtgat ttcgtctgcc
      541 cgaactgcgg ccagcacctg gcgttcgaga actcggtgtg cctgtcgtgc gacaggccgc
      601 tcgggttctc cctcgacgac atggcgctgc tggttatcgc ggagggacct gatgccgcga
      661 agccggggtt cgtggacgcc gccaactacg agctgtgcgc gaatctacat ctggcgcagt
      721 gcaattggtt cgttcagaag gagccggtgc gtcgactgtg cacctcgtgc cggctcaccc
      781 gcacccgccc gaacgacgcg gacaccacgg cgctgtctgc gtttgccgat gccgagaagg
      841 ccaaacgccg gttgatcgcc gagctgcacg agctgcggct gccgatcgtg ggccgcgacg
      901 acgaccccga gttcgggctg gccttcgacc tgctgtccag tgagaaggac aaggtgttca
      961 ccggccacca caacggcgtg atcacgctgg acctcgccga aggcgacgac gtccaccgcg
     1021 agcagttgcg catcgagatg gacgagccgt atcgcacact gctggggcac tttcggcacg
     1081 agatcggcca ctactacttc taccggctcg tcgagccgtc gcaggagtac tccgagcggt
     1141 ttcgggagct gttcggcgac cccgacgccg actaccagga ggcgctggac cgtcattacc
     1201 gcgaaggcgc accgccggac tgggaagaga cctatgtctc gtcctacgcg acgatgcacg
     1261 ccgccgagga ctgggccgaa acgttcgcgc actatctgca catccgcgac accctggaca
     1321 ccgcggccgc gttcagcttc gcgccggcta atgccaccta tgagcggaag gtgttgggac
     1381 ccagcggctt caacacgatc atcgagatgt ggctgccgct gtcgtggggc ctgaacatgg
     1441 tcaaccggtc gatgggtcgc gacgacctgt acccgttcgt gcttccggtg ccggtgctcg
     1501 acaagatgcg gttcatccac acgatcatcg acgaggtgac ggccgatccg acgaaactgg
     1561 ccgcggccac ccccacctca tagcgctcgc ggcagccagc cgcggcggag gctcttgacg
     1621 ttcaagccaa tccacaggca gactcgcgcg gcatgacccc acgcttcggt gttcgcttcg
     1681 gcgcctccgt ctttatcggt gcgctcgctc tgcagctgac gggctgcagc aagaccatca
     1741 agccggaggg cgcggccaaa tccgtgacgg atctcgtctc tgagcagacg cacttcacgc
     1801 cgaacgacgt gaagtgccct tccggggtcg atgccgaagt gggccaggag ttcgactgtc
     1861 acttcaccgg tcctgatggc gcgtacaccg cgcacctgaa agtcacgaag gtcgaaggcg
     1921 acgacgtcga gttctatatc acgaccaagc tcgatcaata gcgcgaactt cgcgatctca
     1981 ccattgctcc tatgtcaaga agttggtttc ccgacgtcgt cgtccaccgt catgaaggcg
     2041 tcgcctgctt cgactcaccc gaccagtggt tcttcaacga catccgcggg tggacgctcg
     2101 ccgaccacgt cgacgacgag cagttcagcc ggttccgcgc tggagcgctt cgtcgatgcc
     2161 gacggccggg tccggttcgc ggcgccggcc ctgatcgcga cggccacccg cccggcttga
     2221 cggcgcgagc gtgcgcgtag ccatgcgccg aaagtgaacc cacggtcgct atttcgccga
     2281 aacaactgcc gtagcttcac tttcggcgtg atttcaatgt cgggtcagcc gcaggaaagt
     2341 cgtgccgcgc ggtgagattc ggtatcccac gtccaggctg atcgtcaact cgaggccctt
     2401 gagctggcgc acccgccgct tgaaccgggg cacgtcgagg ccttcgcgcg ccgccaggtc
     2461 cggcgcgcgc accccctcgt tgtcccggat cagctccaga tagcgccgcg tccacggtcc
     2521 cttgccgctt gcggcgtcga agcgggccaa ccgctcgctg atcactgcga cgtcgtcggc
     2581 cgtcaacgcg tcgttagcgg ccagcgcggg ccgttcgtcg gggccggcgt acgacaccgt
     2641 gatgacgaag acgtgctcgg cggacctgcg atccagttct gcatgcgcgg ccgcggcgcc
     2701 gcgatagccc gcggcccgag cctgcgcctc ggtcacccga tagccagccg ggcgctcgac
     2761 gacgttgtcg atgcggatgg tgccgccggc gatcccgtcg gcggtggcgc gattcagcag
     2821 cacacgacgg atcgtatggc ccgcccgcgt ggtatgcctg agctgtgtct gatgtcgccg
     2881 gccgggcggc tccgaagtcc gacggtctgt tcgaccttcc cggcaccagc gactccgccg
     2941 actggcaggg ccgcggagca ggtttgcccg caccgctggc ggtccggatg cggcccgcca
     3001 cgctcgacga ggtcgtcggg cagtcgcatt tactcaccga gcggtcgccg ctgcgccgcc
     3061 tgatcgacgg ctcgggtgcg gcgtccgtca tcctctacgg gccgcccggc accggcaaga
     3121 ccacgctggc gtcgctgatc tcgcaggcca ccggccgccg gttcgaggcg ctctcggcgg
     3181 tgtcggccgg ggtcaaggaa gtccgcgcgg tgatcgagaa tgcccgccgc gctgccgcgc
     3241 acggtgagca gaccgtgttg ttcatcgacg aggtgcaccg gttctccaag acccagcagg
     3301 acgcgctgct ggcggccgtc gagaaccgtg tcgtactcct cgtggcggcc accacggaga
     3361 acccgtcgtt ctcggtggtg gcgccgctgt tgtcgcggtc gctgatcctg caactcaagc
     3421 cgctctcggc cgacgacatc cgcacgctca tccagcgcgc cgtcgacgac ccgcggggcc
     3481 taggtgggag ggtcgagatc gaacccgatg ccgtcgacct gctggtgcga ctgtccgccg
     3541 gcgacgcgcg ccgggcactg accgcgctgg aggtcgcggc cgaaacggtg accgacgccg
     3601 gcggccccgt gaccgtcgcc agcatcgagg agtcgctcga caaggcggca gtgcgttacg
     3661 accgcgacgg tgaccagcat tacgacgtca tcagcgcctt catcaagtcg gtccgcggct
     3721 cagatgtcga cgcggcgctg cactatctgg cccgcatgct ggtcgcgggg gaggacccgc
     3781 ggttcgtcgc acggcgactg atgatcctgg ccagcgagga catcggcatg gccgatccca
     3841 ccgcgctgca gaccgcggtc gccgccgcgc agaccgtcca gctgatcggc atgcccgagg
     3901 cgcaaatcac gttggcgcac gccacgattc acctggcgac ggcgccgaag tccaatgcgg
     3961 tcgtcgccgc aatcggagct gcgatgagcg acatcaaggc cggcaagacc gggctggtgc
     4021 cagcccatct gcgcgacggt cactactccg gcgcggccgc gctgggcaac gcgatcggct
     4081 acagatattc acatgacgac ccggacggcg ttgtggccca gcagtatccg cccgacgagc
     4141 tggtcggcac cgactactac caccccaccg agcacggcaa cgagcgcgag atcgccaagc
     4201 ggctaaaccg gctgcgcgcc atcatccgca agaggcgcta gtcaccaccg acgggtcagc
     4261 cgtgacgctc cgaaacggcg gcgtccgaac gtggatcgac cgaatgccgc agtaacgagc
     4321 gccacaccga gcaccgcgac gatcacctgg acaagcagct cgccccagtc gacgccgggc
     4381 gtgtcgatcg ggatgcccat tgcccgcgcc agcgcggtgc cgacgaacgc cgacccgatc
     4441 ccgacgagga tggtcagcca aatcggtatc ggctgcttgc cgggcagcac gaatcgtgcc
     4501 agagcgccga ttaccagacc gataaggatt gcgccgagga cgctgtggat tgtcattctt
     4561 cctcccaacg atgagatgca ggccgcatac ccgcgccgga agcgaataaa cgccggggaa
     4621 ttagtttccg cgctgggcgc ggtcacgaat gccgcgcagc atcttctgct ccatcacgaa
     4681 gtgcgggatg tcgaaggtcg cagtacccac cgcgccgccg ctgccacgag caagcagccg
     4741 ggtgcgcttg ccgtcgggaa tcaggtagaa cgcccatgag ccaagcgcct tctcgccgcg
     4801 cgtcaccctg tcgtagtcgg cttcagacat cagcaccaga tgcgacttgg gttcgacggc
     4861 cgcaacgacc tgcctgccca tcgggccata ccggcgggcc agccacaccg agtcgcccac
     4921 ccgcagttcc tgccagtccg gattgatcgt ctgcgcgttg tgaatccggg tgccgacaga
     4981 ccgctccagc cagtcgtagc tgtacccgcc gcggtcctca ccgatctgcg ctagccaggg
     5041 ccacacgtcc ttcacgggcg cgtcgatcgt cacaccgcgg gtggtgcgcg gcgtcaccgc
     5101 cgacaccagt tcgtcgccgg gcagcaccgc actcagctcg tcaggcgagg ccccccaggc
     5161 gtacatccac gggcggatcg cgcgtcggta cagctccagg ccgcccatcg ccccggccgc
     5221 cccggccagc catcgtcgtg cgttcgccat acgacgcgac gctagccacc gccgactttt
     5281 cggcgtgagg gtcgaaagtc ctcagcttct gcggcggcga acgcccttga gcgctgaaag
     5341 acctgtgatc gaacgccgaa cgtgaagcca gggctgctga ttggcggcga tcacagccct
     5401 ggtttctgtc tcggcgcgcc agaacgcgtc agatcaactc aggtacccgc agatgcgcga
     5461 tcgccagctc gaattcgccg acgtcgagct ggtcagcgcc tagctcccgc agccgcgccg
     5521 tccgcaacga cctggcctgc tcgcggttcc ggtccatcgc gtcggcgctg tcgaacgtca
     5581 ccgacgacac cgcacgcccg gacgaccggt cgaccaacag gcttgcgctg cagaaccctt
     5641 cgagctcttc caacgccggc agcacggagt ccttgtataa ctcgaccgac ctgccgccct
     5701 gcccgttggg gactttgaac cacgtcgcgc gcacacaggc accctcaccc gagcggtggt
     5761 ctcgatgcag cacggcgatc tcccattctt ggacggttgc ggtgcccccg aggatgtccg
     5821 cggcccggtc gcgaacgggt gcgacctgct cggcgctggc acgcattgca tcttcggatt
     5881 cccaagcggt ggtggtgatg cacctgccgg tttcccggtc gaccatcaac gagatgccga
     5941 tgcagccgtg catggcctcg agtgcgggca tgacctcgtc gcgaacgtgc gcaattccgg
     6001 cgtcgatcgc ctcgggtcgc gcctcaatag tggtagatcg tgcgaacacg gccgaccccc
     6061 tctgtgttcg gggcggcgcc ccggtggcgc caccggtccg ctgatcaggt tcctcctctg
     6121 caccgagcct ggcaatgggg gcgaacgtag gctggaagcg tggataccgc cgttctcgac
     6181 gtcgacaccg cgcgtcgccg catcgtcgat ctcaccgacg aggtgcgctc gttttgcgcc
     6241 tcgcacggtg acggcttgtg caacgtgttc gtgccgcacg ccaccgcagg cgtcgcggtc
     6301 atcgagaccg gcgcaggaag cgacgacgac ctcgtcgaca ccctggaacg gctgttgccg
     6361 cgcgacgacc gctaccgcca cgcgcacggt tcgccgggcc acggcgccga ccacgtgctg
     6421 cccgccctga tcacgccatc ggtgacgatg cccgtccaga ggggtaagcc cctgctggga
     6481 acctggcaga gcgtcgtgct ggtggacctg aaccgggaca acccccggcg gtcggtgcgg
     6541 ctgagcttcg tcgacggttg acgtcggcaa caaaccaggc cggtaatgta gtcgggtcaa
     6601 cggggcccac gtcagaaaag acaacgacag taagtgcaga cacacgaaat caggaagcgg
     6661 ttcaccgacc acttcgtgaa ggcgggccac accgaggtgc ccagcgcgtc ggtgatactt
     6721 gacgacccca atttgctgtt cgtcaacgcc ggcatggtgc agttcgtgcc gtacttcctg
     6781 ggccagcgca caccgccaca tcgcaaagcg accagcatcc agaagtgcat ccgcactccc
     6841 gacatcgacg aggtgggcgt caccacccgg cacaacacgt tcttccagat ggccggcaac
     6901 ttcagcttcg gcgactactt caagaggggc gcgatcgagc tggcctggac gttgctgacc
     6961 aacccgatcg aggacggcgg atacgcgctg gacccggaaa agctctgggc gacagtatat
     7021 ttggacgacg acgaggccgc gggtctgtgg caggagatag cgggcctgcc gcaggagcgc
     7081 atccagcgcc gcggcatggc cgacaactac tggtccatgg gcattcccgg gccgtgcggc
     7141 ccggcatcgg agatctacta cgaccgcggt cccgaatacg gccccgaagg cggcccggtc
     7201 gccagtgagg accgctacat cgagatctgg aatctcgtgt tcatggagaa tctgcgcggc
     7261 gacggaacgt cgaaaaccga cttcgaaatc cttgggccgt tgccgcgcaa gaacattgac
     7321 accgggatgg gtgtcgagcg gatggcgtgt gtgctgcagg gcgtcgacaa cgtctacgag
     7381 accgacctgc tgcgcccggt gatcgacaag gtcgccgaga tcgcgccgcg gggctacggc
     7441 aagggcaacc atgatgacga cgtgcgctac cgcatcatcg ccgaccacag ccgcaccgcc
     7501 gccatcctga tcggcgacgg cgtcagcccc ggcaacgacg gacgcggata tgtgttgcgc
     7561 cgcttgcttc gccgcatcgt gcgcgcggcc aagctgctcg ggatcgacac cccgatcgtc
     7621 ggtaagttga tggccaccgt gcgcgatgcg atggggccgt cgtatccgga actgattacc
     7681 gacttcgcgc ggatcacccg catcgccgag gcggaggaga ccgcgttcaa ccgcacgctg
     7741 gcgtcgggct cgcggctgtt cgaggaggcg gcgagcgcga cgaaggccgc cggcgcgaag
     7801 gtgctcagcg actcggatgc gttcacgctg cacgacacct acggattccc gatcgacctc
     7861 acgctcgaga tggccgccga ggccgggctc gcggtcgacg agaacggctt ccgcaggctg
     7921 atggccgagc agcgcgagcg cgcgaaggcc ga
//
LOCUS       DASWBF010000191         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1066133,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000191 DASWBF010000000
VERSION     DASWBF010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066133"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1636
                     /locus_tag="VF874_06865"
     CDS             227..1636
                     /locus_tag="VF874_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX9175820.1"
                     /translation="MLDRRHFLMLSAVSVAAACSRTGDPAGRKAVDVALTAGETDLRV
                     GGLQWRTWAYDGQVPAKEIRLRKGQTPRAAVTNNLPADTSVHWHGLAIPNPMDGVPPL
                     TQQPIAAGQRFTYEFVVPDSGTYWFHSHVGTQLDRGLYGPLIIEDPDERADYDDELAL
                     VLDDWIDGTGTNPDKVFEQLQKTGMPSMGPMGGDAGVTPTTPLGDDGGDVTYPYYLIN
                     GRIPGDPQTVDYRAGQRVRLRIINAGSDTAFRVGVPNTKMTVTHTDGFPVIARDTDSV
                     VLGMGERVDATVTVNSSVPVIAAAERKDGYAQLNLRANGTPSNVNVEQFVTTLRNSQP
                     LDTATLSAAPEVQLPQRKPDQTFDLRLAGPVPPYTWTINGQVYDPTKDGLPVKPGQRV
                     QVRYINESKMFHPMHLHGHTFQVMSNGRPMARKDTVLVPPLATVDTVFDANNPGKWID
                     HCHNTYHLESGMAVYFTYT"
     gene            complement(1728..2255)
                     /gene="purE"
                     /locus_tag="VF874_06870"
     CDS             complement(1728..2255)
                     /gene="purE"
                     /locus_tag="VF874_06870"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX9175821.1"
                     /translation="MASSPRVGLIMGSDSDWPVMEAAAHALAEFDIPFEVGVVSAHRT
                     PARMLDYARDAAGRGIEVIIAGAGGAAHLPGMVASATPLPVIGVPVPLAKLDGLDSLL
                     SIVQMPAGVPVATVSIGGARNAGLLAVRILGASDPQLRKRIEDFQRQLEASVLQKDTA
                     LRDRLLGPDGNVAGD"
     gene            complement(2245..>2576)
                     /locus_tag="VF874_06875"
     CDS             complement(2245..>2576)
                     /locus_tag="VF874_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009479505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX9175822.1"
                     /translation="RAVLDYPLGATAPVVPLTVMANVLGAPQPPTMTMDERLHHLYAR
                     MPDARVHLYGKAERPGRKVGHVNMLGRHLSPDSEEDVAALWERAERAAHWLSHAEWTD
                     GWDAHGL"
BASE COUNT          460 a          931 c          823 g          362 t
ORIGIN      
        1 ggtgcaggtc gcgcatccgg agccgtcggc gctggaaagg caccgcatca ccgagaacgt
       61 caccgtcgcg ctgtaactct gcgcgagcgt gcgcgtcatg cctgggggca cctcccgctc
      121 gcgggggacg acacgccgct aagtgcggca ttttgaggca cactcgcgcc gggtgatttg
      181 acgcatttgc gcccgcacct cccccgtcgg tgcgaacctt aagacaatgc tcgatcgacg
      241 gcacttcctg atgctttccg cggtttcagt ggcggcggca tgctcccgga ccggcgaccc
      301 cgcgggccgt aaagccgtcg acgtcgcgct gaccgccggt gagacagacc tgcgcgtcgg
      361 cggcctgcag tggcggacgt gggcctacga cggacaggtg cccgccaagg agatccggct
      421 ccgcaagggc caaacgccgc gcgccgcggt gaccaacaac ctgcccgccg acaccagcgt
      481 acactggcac gggcttgcga tcccgaaccc gatggacggg gtgccaccgc tgacgcagca
      541 gccgatcgcc gcgggtcagc gcttcaccta cgagttcgtc gtccccgaca gcgggaccta
      601 ctggttccac tcgcacgtcg gcacccaact ggaccgcggg ctctacgggc cgctgatcat
      661 cgaggacccc gatgagcgcg ccgactacga cgacgagctg gctctcgttc tcgacgactg
      721 gatcgacggc accggcacca acccggacaa ggtgttcgag caactgcaga agaccgggat
      781 gccgagcatg ggacccatgg gcggggacgc gggcgtgacg ccgacgaccc cgctcggcga
      841 cgacggcggc gacgtgacct atccgtacta cctgatcaac ggccgcatac ctggcgaccc
      901 gcaaacagtc gactaccgag cgggacaacg ggttcggctg cggattatca acgccggctc
      961 cgacaccgcg ttccgcgtcg gcgtgccgaa cacgaagatg accgtcacgc acaccgacgg
     1021 cttccccgtc atcgcacgcg acaccgactc ggtggtcctc ggcatgggcg agcgcgtcga
     1081 cgccacggtc acggtgaact cgtcggtccc ggtgatcgcg gccgccgagc gcaaggacgg
     1141 ttacgcgcaa ctcaacctgc gcgcgaacgg gacgccgagc aacgtcaatg tcgagcagtt
     1201 cgtcaccacg ctgcgcaact cgcagccgct agacacagcg acgttgtcag ctgctccgga
     1261 agtgcaactg ccgcaacgta agccggacca gaccttcgat ctgcggctgg ccgggccggt
     1321 gccgccgtac acgtggacga tcaacggcca ggtgtacgac ccgaccaagg acggcctccc
     1381 ggtcaaaccc gggcagcgcg tgcaggtgcg ctacatcaac gagtcgaaga tgttccaccc
     1441 catgcacttg cacgggcaca cgttccaggt gatgagcaac ggccggccga tggcgcgcaa
     1501 ggacaccgtg ctggtcccgc cgctggccac ggtggacacc gtgttcgacg cgaacaaccc
     1561 cggcaagtgg atcgaccact gccacaacac ctatcacctc gagtcgggca tggcggtcta
     1621 cttcacgtac acgtaggcgt ccacccaccg cgaggggccc acttcagcaa ggcgcgcgac
     1681 atcaccacgc gctggatcgg ggttgccagc catgtcagga ctccttgcta atcgccggca
     1741 acgttaccgt cgggcccgag cagccggtcc cgtagggcag tgtccttctg cagaaccgac
     1801 gcctcgagct gacgctgaaa atcctcgatc cgcttgcgca actgcggatc cgacgccccc
     1861 aggatccgta ccgccagcag cccggcgtta cgggcgccgc cgatcgacac cgtcgccacc
     1921 ggcaccccgg ccggcatctg cacgatcgac agcagcgagt ccaggccgtc cagcttcgcc
     1981 agcggcacgg gcacgccgat caccggcagc ggagttgccg acgcgaccat gcccggcaaa
     2041 tgagcagcac ctccggcccc ggcgatgatc acctcgatac cgcggcccgc cgcatcccgc
     2101 gcgtagtcga gcatccgagc gggggtgcga tgcgcggaga cgacgccgac ctcgaacggg
     2161 atatcgaact cggccagcgc gtgggcggca gcttccatga cgggccagtc gctgtcgctg
     2221 cccatgatca agccgacccg ggggctagag gccatgcgcg tcccatccgt cggtccactc
     2281 cgcgtgcgac aaccagtgtg ccgcgcgttc cgcccgttcc cacagggccg ccacgtcttc
     2341 ttcggagtcc ggtgaaagat gccggcccag catgttgacg tggccgacct tccgcccggg
     2401 ccgctccgcc ttgccgtaca ggtgcacccg cgcgtcgggc atgcgggcgt agaggtggtg
     2461 caaccgctcg tccatcgtca tcgttggcgg ctgcggtgcc cccagcacgt tggccatcac
     2521 ggtcagcggc accaccggcg cggtggcccc aagcggatag tccaggacgg cacgca
//
LOCUS       DASWBF010000192         3757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1000732,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000192 DASWBF010000000
VERSION     DASWBF010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000732"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF874_06880"
     CDS             <1..181
                     /locus_tag="VF874_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175823.1"
                     /translation="EADHPTGIFKSVAHLIRRINDSIANWNSDCKPFTWTADAASILA
                     KVKFVESQVRAITGH"
     gene            complement(302..1693)
                     /locus_tag="VF874_06885"
     CDS             complement(302..1693)
                     /locus_tag="VF874_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9175824.1"
                     /translation="MSIVDDLRSAGATRVLEPGEAGYDEAVAGFDTAVVVAPAVVVDA
                     QTASDVAATVAVAAEDGRTVTVLGAGHGRLHDVRDGLALAVRRLDTVDIDVAARTARI
                     GAGCTWEPVLAAATPHGLAPVCGSAPGVGVAGYLLGGGLGPLASGLGFSSDYLRSVDV
                     VTPADGPVTVTADSHLDLFWALRGGKGGFGVVTSVTVDLLALAEVVGGGLYFAADDVA
                     AVLHAYAEWAARLPESSTTSVALLRLPSSDALPDQIRGKHVVHVRFASLDSASAASAQ
                     LGDLRAVARPLLDTVAVLPYGELGSIHGDPSTPMPVANGAAPLAALDARTVDAVLAAA
                     DLAADLPLSSVEIRTLGKATHLSPSRGDAVGGRATPHLLNVYAAPVQTLSDDQRLATI
                     RDVLGAVAPWRAPVNLVNFVGRGNDSAAIVDSWTTEQNEQLDVVRDRYDPERLFPFAR
                     HVSDAGEAPKGRS"
     gene            complement(1808..3418)
                     /locus_tag="VF874_06890"
     CDS             complement(1808..3418)
                     /locus_tag="VF874_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9175825.1"
                     /translation="MSRTRTGAELVQDFISTVEIEYVFGNPGTTETTFLAALAGSQAS
                     YVLALNEPSAVGIAAGYSLATGKTAMVSLHTFPGLASGMFNLRNAYLSGIPLLVVNGT
                     EDSRFLIHNPVLGGPNTQLAETATKYQYEARSIDELTVALQRCWTQAGVQPTRPVFLS
                     VPMDFMAGSTERIAFKRTVVHDDIVSASIADVATALRSSRRLAIVVDYAVGWDDSVRA
                     LTNVAAALEADVYAAPFHVQGVCDMLHPTFRGALPPTTREVRDILSGYDTVLLLGEKI
                     DTFTYTGEQAVPPEVRLIQIAPATDQLGFDWPVDIAVVGDISTSLTGIGTALGVDVAA
                     PIVDTSPDLAVLRTKFTRSDDDPSNGLIFAVLEQLDPASSHVVTEGSSEDQLVQQMAT
                     SMGFRNVHFSPRGGALGWAMPLAVGLTLGTGQPAVCFVGDGGSLFSVHAIWTAAALKL
                     PVIVVCFVNHEYRLLKDLWVSFTGGTFDTTRFVGLDFNDPALDWEGIMRGFGAVTARP
                     TTVDDVVEVVAAARQRQGPTVLLVDRRP"
BASE COUNT          610 a         1232 c         1353 g          562 t
ORIGIN      
        1 cgaagcagac catccgacgg ggatcttcaa gtcggtcgcc cacctcatta gaaggatcaa
       61 cgactccatc gctaactgga acagcgactg caagccgttc acgtggactg ccgacgcggc
      121 aagcatcctg gcgaaggtga aattcgtgga gtcacaggtc cgcgcgatta cgggacacta
      181 gattgcccac ttgggtattg ctcgtcctcc gggctcgtgg atgaaccgga caggccaaga
      241 cgttgtgaca catcacagtc ttgggtgaga tcaaggcatc cgagcgaggc cgatcgctag
      301 ttcacgagcg gcctttcggc gcctcaccgg cgtctgacac gtgtcgggcg aaggggaaca
      361 gccgttcggg gtcgtaccgg tcccggacca cgtcgagctg ctcgttctgc tcggtcgtcc
      421 acgagtcgac gattgcggcg ctgtcgtttc cgcgcccgac gaaattcacc aggttcaccg
      481 gtgccctcca cggcgcgaca gcgccgagca cgtcgcggat cgtggcgagc cgctggtcgt
      541 ccgaaagcgt ctggactggg gccgcgtaga cgttgaggag gtgcggggtg gccctgccgc
      601 cgacggcgtc gccgcgggac gggctgaggt gggtggcctt tccgagggtc cggatctcga
      661 ccgacgacag cggtaggtcc gcggcgaggt cggccgcggc taggacagcg tcgacggtcc
      721 gggcgtccag ggcggccagc ggcgcggcgc cgttggccac cggcatgggt gtggacgggt
      781 cgccgtggat gctgccgagc tccccgtagg ggaggacggc gacggtgtcg agcagcgggc
      841 gggcgacggc gcgcaggtcg ccgagctgcg cgctggcggc gctggcggag tcgaggctcg
      901 cgaaccgcac gtggaccacg tgcttgcccc ggatctgatc gggcagggcg tccgacgacg
      961 gtaggcgaag cagggctacc gacgtggtgg acgactcggg aaggcgggct gcccactcgg
     1021 cgtaggcgtg cagcacggcc gcgacgtcgt ctgccgcgaa gtagaggccg ccaccgacga
     1081 cctcggccag ggcgagcagg tcgacggtca cggaggtgac gactccgaag ccgcccttgc
     1141 ccccgcgcaa tgcccagaac aggtcgaggt gggagtcggc tgtgaccgtg accggcccgt
     1201 cggcgggcgt gaccacgtcg accgagcgca ggtagtcgct gctgaagccg agtccgctgg
     1261 ccagtgggcc cagcccgccg cccagcaggt agcctgcgac gcccacgccc ggtgccgaac
     1321 cgcacacggg ggcgagaccg tgcggcgtcg cggccgcgag caccggttcc caggtgcacc
     1381 cggcaccgat ccgcgcggta cgggcggcga cgtcgatatc gacggtgtcc aggcgccgga
     1441 ccgcgagcgc cagaccgtcg cggacgtcgt gcagacggcc atggccggca ccgagcacgg
     1501 tcaccgtcct accgtcctcg gccgccacgg cgacggtcgc cgccacgtcg gaggcggtct
     1561 gagcatcgac caccacggcg ggcgccacga cgaccgcggt gtcgaatccg gccacggcct
     1621 cgtcataccc ggcctcaccg ggctcgagga cgcgggtggc ccccgcggat cgcaggtcgt
     1681 cgacgatgga cacggcatct cccgggtgag gcggtggaat ggttatggat cggtcagagt
     1741 gcgcccgcca gcagcagacg agcccgtcgg tcatcctgca cgtcgcagcg cgacgtccgg
     1801 aggtcagtca gggccgacgg tccactaaca gcaccgtcgg accctggcgc tgtcgggctg
     1861 cggccacgac ctcgacgacg tcgtcgaccg ttgtgggcct tgcggtgact gcaccgaacc
     1921 cgcgcatgat gccctcccag tcgagcgcgg ggtcgttgaa gtcgagaccg acgaacctgg
     1981 tggtgtcgaa ggttccgccg gtgaagctca cccacaggtc cttgaggagc cggtactcgt
     2041 ggttgacgaa gcacacgacg atgaccggga gcttcagggc cgcagcggtc cagatcgcat
     2101 ggacggagaa caggctgccg ccgtcgccga cgaaacacac cgccggttgg ccggtgccca
     2161 gcgtgaggcc gacagcgagc ggcatcgccc acccgagcgc tccgccgcgg ggactgaagt
     2221 gcacgttgcg gaatcccatc gaggtcgcca tctgttgcac gagctggtcc tcggacgacc
     2281 cctcggtgac gacatggctc gacgccgggt cgagctgctc gaggacagcg aagatgagcc
     2341 cgttggatgg atcgtcgtcg gacctcgtga acttggtgcg caggactgcg agatcgggac
     2401 tggtgtcgac gatcggcgcg gcgacgtcga ctcccagcgc ggtcccgatg ccggtcaggc
     2461 tcgtcgagat gtcgcccacg accgcgatgt cgacaggcca gtcgaagccg agctggtcgg
     2521 tcgccggagc gatctggatc aggcgcacct cgggcgggac cgcctgctca ccggtgtagg
     2581 tgaaggtgtc gatcttctcg ccgagcagca gcaccgtgtc gtagccagac aggatgtcgc
     2641 gcacctccct cgtggtcggc gggagcgcgc ccctgaaggt cgggtgcagc atgtcgcaga
     2701 cgccctgcac gtggaagggc gcggcataga cgtcggcctc gagcgccgcc gcgacgttgg
     2761 tgagtgcccg gaccgagtcg tcccaaccga cggcgtagtc gacgacgatc gccaaccgcc
     2821 gcgaggagcg caatgcggtc gctacatcgg cgatcgaggc gctgacgatg tcgtcgtgca
     2881 cgaccgtgcg cttgaacgcg atgcgctccg tcgagccggc catgaagtcc atggggacgg
     2941 acaagaacac gggccgcgtc ggttgcacgc ccgcctgtgt ccagcaccgc tgcagggcca
     3001 ccgtcagctc atcgatactg cgggcttcgt actggtactt cgtcgcggtc tctgccagct
     3061 gggtgttggg gccgccgagg accgggttgt ggatcaggaa ccggctgtct tcggtgccgt
     3121 tgacgacgag gagggggatc ccgctgagat aggcgttgcg gaggttgaac atgccgctgg
     3181 caaggcccgg gaaggtgtgc aggctcacca tcgccgtctt gccggtggcc agcgagtacc
     3241 ccgccgcgat cccgaccgcg ctcggttcgt tcaacgcgag gacatagctc gcctgcgatc
     3301 ccgccagggc agcgaggaac gtcgtctccg tcgtgccggg attgccgaac acgtactcga
     3361 tctcgacggt cgagatgaag tcctgaacca gctcggcacc agtcctggtc ctgctcatga
     3421 gggtcttcct tccatgatcg tctcgaggac gtggtgggag tcctccgcgc acgcccggga
     3481 acgggtcgtg aagcaccccg gcacgtcgga gcccgcagcc gcctgtgcaa cctgtggtcc
     3541 actggcccgg gtgatcatcg ggtcacacgt ctgcctgtcg gtcgctcatc gtcagcgagc
     3601 cacgtcgcca gccgtaggcg gcgtagctgg gccaccggcc gtccacagcg cgcgcaggtc
     3661 gcggagcacc cacatgagcc gggcgtcggg gatcacgtac ccaccgacac cggtgctgtg
     3721 gaggaggtcg aagactcggc ctgagacctg tgcgagt
//
LOCUS       DASWBF010000193         8160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_481033,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000193 DASWBF010000000
VERSION     DASWBF010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481033"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2924)
                     /locus_tag="VF874_06895"
                     /pseudo
     CDS             complement(1..2924)
                     /locus_tag="VF874_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898426.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
     gene            3114..4595
                     /locus_tag="VF874_06900"
     CDS             3114..4595
                     /locus_tag="VF874_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX9175826.1"
                     /translation="MGEEVKATTFTGAQRRDYRRKVQLSLDVFETMLAQSSFDFERPL
                     TGMEIECNLVDEHYHPAMANREVLAAIADPAFQTELGAYNIEFNVPPRPLPGESGLEL
                     EADVRASLNAAEAKATQHGAHIVMIGILPTLMPEHLAGEWMSESVRYRALQESVFMAR
                     GEDIAIDISGPERLSLQMASIAPESACTSMQLHLQVSPAEFADNWNAAQVLAGPQLAL
                     GANSPYFLGHQLWAETRIELFLQAIDTRPDELKAQGVRPRVWFGERWITSIFDLFEEN
                     VRYFPSLLPELSDEDPVAELAAGRAPQLHELRLHNGTIYRWNRPVYAVVHGRPHLRVE
                     NRVLPAGPTVIDMMANAAFYYGALRALSEDDRPLWTQMSFAVAQENFVNAARYGMAAR
                     LYWPGMGTVTADELVLRQLLPLADEGLRRWQVAAAVRDRFLGVIEGRAKAGVNGAAWQ
                     VQTVTALQEGDGLTRPQALAEMLRRYCEHMHSNEPVHTWELPR"
     gene            4695..5369
                     /locus_tag="VF874_06905"
     CDS             4695..5369
                     /locus_tag="VF874_06905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175827.1"
                     /translation="MSEVMNWDDAYQEQGFFEGPPPWNIGEPQPEIAELIRAGKFRSD
                     VLDAGCGVAEASLALAAQGHTVVGIDLTPTAVAAASEAAQERGLSNATFVQVDITSFT
                     GYDGRFNTILDSTLFHSLPIEARDDYLRSVHRAAAPGASYYILVFATGAFPADADFGP
                     HVVTEDEQRQAVSKYWDIDEIRPAYIHANMPSMPGMPEPPMTFDVDEKGRQKMPAFLL
                     TAHKAS"
     gene            complement(5347..6465)
                     /gene="egtE"
                     /locus_tag="VF874_06910"
     CDS             complement(5347..6465)
                     /gene="egtE"
                     /locus_tag="VF874_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis PLP-dependent enzyme
                     EgtE"
                     /protein_id="HEX9175828.1"
                     /translation="MISGTRWRDARPAVKGVHLDSAACSRQSFAAIDAAAACARREAE
                     VGGYVAAQEADAVLAVGRIGVAALTGMSADDVVFTTGATNALDLLLRAWPDERRVIAC
                     VPGEFGPNLGVMEVNGYDVALLPVDSLGRVVVEDLAAQWAREIPSLVHLTVVPSHRGI
                     VQPLREIAETCREYGVPLVVDAAQGLGHVDCAVDADAVYGTSRKWLAGPRGVGVLGVR
                     ADRAASLRPRVSQHWADIPPLRQLEVGEANIAARVGFSSAILEHMDDGPTKVRAGLAE
                     AGRLTRTVLSDLAGWRVVEPLDEPTAITTLAPTDGADVAQIRAELISRHAIVTTVAGP
                     DRAPFELSTPVLRLSPHVDVSEDDLTTFARALSSLCAR"
     gene            complement(6462..7427)
                     /gene="egtD"
                     /locus_tag="VF874_06915"
     CDS             complement(6462..7427)
                     /gene="egtD"
                     /locus_tag="VF874_06915"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX9175829.1"
                     /translation="MSVMLANHLAADSANRALRRDVYHGLRQTPKSLPPKWFYDDVGS
                     DLFDQITRLPEYYPTRAEAAILRAEAASIADVTGADTLVELGSGTSEKTRMLLDALHR
                     CGTLRRFIPFDVDAGVLQTAGGAIVAEYPGIEVDAVCGDFEEHLTEIPGGGTRLLAFL
                     GSTIGNLTPGPRAEFLATLAAVMAPGDSLLLGTDLVKDVDRLVRAYDDSAGITAAFNR
                     NVLAVVNRELDADFDLDAFEHVSRWNVEEERIEMWLRSIGAQRVRVGALDLDVDFDAA
                     EEMLTEVSCKFRRETVTAELAAAGLTLTQWWTDPDGDFGLSLAAK"
     gene            complement(7424..8119)
                     /gene="egtC"
                     /locus_tag="VF874_06920"
     CDS             complement(7424..8119)
                     /gene="egtC"
                     /locus_tag="VF874_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HEX9175830.1"
                     /translation="MCRHLGWLGEPHSLASLVLDPPYGLGVQSYSPRRQKHGLLNADG
                     WGVGFFDTEGVPRRWRSAGPLWSDASFASVAPVLRSGCVVAAVRSASVGMPVEASASA
                     PFSDGRWLLSHNGLVDRAVLPPSPKAESVVDSALLAALIFERGLDVLADTITEVGTAD
                     PNARLNILAADGSRLLATTWGDTLSVLHGDDGIVLASEPFDDDPAWQDVPDRHLVEVS
                     RGDVTLTPLKGVR"
BASE COUNT         1417 a         2826 c         2625 g         1292 t
ORIGIN      
        1 ccacaggtat tgacaattgc tctcagcttg ttgtagacgt ggcgcaagtg cgactggatc
       61 gttcgtggcg agacgaacag ccgggtggca atgtccttgt tgggcagtcc ttcgccgacg
      121 aggcggacga cgtcgagctc cgtcggcgtg agcgatcccc acacaggtat ccggccatgg
      181 gcgcggcgat ggcgaggcct tcgtacgcat ccgccgcggc ctgctcgttg ttgccctccg
      241 caacgtggac acgggtccgc gctgccaacg ccatacacag gtacatgccg ctggtcgccg
      301 cagcagcctc gtccgcccag cgtcgtgcac gagctgtatc tccacatgcc aaggcggcgt
      361 gagccgccca cacaaagggg gtcttttcca ccgcggcgtt cgtgtcactc cgcaacgcgg
      421 tctcactcgc ctccaaagcc gcagcggcat tgccggcagc caactcggcc acgacaacat
      481 cgcagtaggc gttccccaag agaaagccca attgggcaca ggcttccagc gctgccccag
      541 ctgtcgtccg agcgccgtcg acgtcaccgt ggtatgccag cgcaaatccc ccggtaatca
      601 aaccggacac ggtattcatg acatcgtgtg caccgatcgc ctcggcgatg agctccgcgt
      661 gttgactcac cgacatggcc atctcgccgc ggctcaggtg tgcccacgcg atggtcatgc
      721 ggcactgtcg ggagttgaag tggtcgccga tggcctccgc caagtcgcgc ccttcctcgg
      781 cgagcgccgc agccgcggtg acgtcgcccc cgagcagggc accgctcgat tgccggccca
      841 gtatctggct cagtcgccat gaatcgccca gggcccgcgc gagttcgact gcctcagtga
      901 agtagggccg ggccagatcg gggtcgtgca tggtgatgaa cgcacacgcg gtgagcaccc
      961 gagtcagcag cgtgggctcg tcggcgtcgc gcgcgatcgc cagagcctgt tggaccggct
     1021 cccacattcc ggtgatatcc acccacgagc tcaatacagc cctatcggca atcgcccggg
     1081 cccatatcgc tggctcactg acttgactct cggccccggc ggcatccagc cacgccagcc
     1141 cttccgagat ccggccgcgc gcgagccaga tgggcagtag cgacgaggcg agctcaccag
     1201 ccttcgtgat gtcgtcgttt tcgcgactcc aggtgaaggc cgcacgcagg ttgtcgatct
     1261 cgttttccgc ttgtgcgagc aactgctcgt agtcattgcg ggccggagca tccaaccgct
     1321 ccgccagtgc cgtgtaatgg tcgcggtggc gtgtgcgaac ctggtcggct tcaccggact
     1381 cgccgagctt ctccaacgcg tactggcgca cggtttccag caggcggtat cgggtccgac
     1441 cgtcggcgtt ttcggcgacg accagagatt tgtcgacgag cagactgagt tggtcgagca
     1501 cctgaaaccg ctccacatcc gtaccaccgg caaccgcttg ggcggcgtcc aggtcgaatc
     1561 cgcccatgaa caccgccagc cgccggaaca gtgcacgttc gtgctccgtc agcaaggcat
     1621 gcgaccagtc gactgacgcg cgcaaacgtc tgctgacgac gcaccgccgt gcgcgcaccg
     1681 ccggtgagca ggcggaaccg gtcatgcagg ctgccgacga tctcgtcaag tgtgagcgcc
     1741 cgtacccgcg ccgcggcgag ttcgatggcc agcggcatgc cgtcgagccg acgacagatc
     1801 tcggtcactg tcacggcgtt ggcggcggtc accgagaagt cgggccgcgc acgtcgagcc
     1861 cggtcgctga acaactcgat ggcctcgtca gccaaggcga gcgacgggac ctgccaactc
     1921 acctcgcctg ccaccaagag gggttcgcgg ctcgtcgcga ggatcgtcac gccggaggag
     1981 gcggccagca ggtcggtgac cagttcggca ctggcctcga ggagatgctc gcagttgtcc
     2041 aacacgagga gcatccgacg gttgcggacg aaatgctgca gcgtatcgat ggtcgagcga
     2101 cccggttggt cgggcaggcc cagcacacga gcgaccgcga ccggcaccac ggcgggatgg
     2161 gtgatcgggg ccaggtcggc ataccagaca ccgtcgggat aaccaggcgt cagttcaccc
     2221 gcgacctcaa ttgccagacg tgtcttgccg gccccgccgg cacccgtcag agtcacgagg
     2281 cggttatcgc ccaggagtgc gcggatctcg gtgatctgcg ctgttcgtcc cacgaagctg
     2341 gtcagctgca tcggaaggtg atggacgaca acggcttccg acgtacgcag tggcgggaag
     2401 tcgttgccga tatcgggatg gcacaactgc gcgacgcgga ccgggcgcgg caggtcacgt
     2461 agccggtgcc tgccaaggtc ggtgagccac acgtcgtcgg gcagccggtc ttcgaccaat
     2521 tcctccgttg cgccagaaag cacggtttga ccgccgtggc ccagatcgcg cagacgcgcg
     2581 gtcttgttga tcgtcggccc ggcatagttg ccgtcgtcgc gcagctgaac ttcgccggtg
     2641 tgcacaccga tccgcaactt gatcggcgcc agcccggctc gctgcagatc cagcgcacac
     2701 gccaccgcgt cgctggcacg cgtaaacgcc gcgacgaagc tgtccccctc gccctgctcg
     2761 accgggcgga caccaccgtg tttggcgatc agctcgccga ccgttcgatt cagcagcgcg
     2821 accgcggccg tcatcgcctc cggctggctt tcccacagcg tggtcgagcc ctcgacgtcg
     2881 gcgagcagca acgtcaccgt tccggtcgga agcagctcgc ccatgtcaac gtcgctccag
     2941 tcagtcgcgc tcatgctagc cagcatgcgg cggcgacgaa cggcaaaaca tcagcccaaa
     3001 ggcgcatttt caccgactcg atcagcgtcc accagccccg cgtgcgtgcg cggaatacca
     3061 gcgttatcgg cgcgtcgttg acacgacacg cacggtcgcg ttgcatcggc ggtgtgggcg
     3121 aggaagtcaa ggccaccacg ttcaccggcg cccaacgccg ggactaccgg cgcaaggtgc
     3181 agctgtcgct ggacgtgttc gagaccatgc tcgcgcagtc cagcttcgac ttcgaacgcc
     3241 cgctcaccgg catggagatc gagtgcaacc tcgtcgacga gcactaccac ccggcgatgg
     3301 ccaaccgcga ggtgctcgcc gcgatcgccg accccgcgtt tcaaaccgaa ctcggcgctt
     3361 acaacatcga attcaacgtt ccgccccggc cactgcccgg tgagtcgggg ctcgaactcg
     3421 aagccgacgt gcgcgcgagc ctgaacgccg cggaggccaa ggccacccag cacggcgcgc
     3481 acatcgtgat gatcgggatc ctgccgacgc tgatgcccga gcacctggcc ggcgagtgga
     3541 tgagcgagtc ggtgcgctac cgcgcgctgc aggaatccgt tttcatggcc cgcggtgaag
     3601 acatcgccat cgacatttcc ggtcccgagc ggttgagtct tcaaatggct tccatcgcac
     3661 cggaatccgc gtgcactagc atgcagctgc atctgcaggt gtcgcccgcc gagttcgccg
     3721 acaactggaa cgcggcccag gtgctcgccg gaccgcagct ggcgctgggc gccaactcgc
     3781 cgtactttct cggccatcaa ctgtgggcgg aaactcgaat cgagttgttc ttgcaagcca
     3841 ttgatacccg gcccgacgaa ctcaaggcgc agggggtgcg cccgcgagtg tggttcgggg
     3901 agcgctggat tacgtcgatc ttcgacttgt tcgaggagaa cgtgcgctac ttcccgtcgc
     3961 tgctgcccga gctctccgac gaggacccgg tcgccgaact ggcggccgga cgggccccac
     4021 agctgcacga gctgcggctg cacaacggca cgatctaccg gtggaaccgg ccggtgtacg
     4081 ccgtcgtaca cggccgtccc caccttcgag tggagaaccg cgtgttgccc gcggggccaa
     4141 ccgtcatcga catgatggcc aacgcggcgt tctactacgg cgctctgcgc gcgctttccg
     4201 aggacgaccg gccgctatgg acacagatga gtttcgctgt ggcacaagag aatttcgtca
     4261 acgcggcccg ttacggcatg gcggcgcggc tgtactggcc gggaatgggc acggtcaccg
     4321 ccgacgagtt ggtgctgcgg cagctgctgc cgctggccga cgaggggctg cgccgctggc
     4381 aagtggccgc cgcggttcgc gaccgcttcc tcggcgtcat cgagggtcgt gccaaggccg
     4441 gcgtcaacgg tgcggcctgg caagttcaga cggtcaccgc gctgcaggag ggcgacggcc
     4501 tgacacggcc gcaggcgctg gccgagatgc tgcggcgcta ctgcgagcac atgcacagca
     4561 acgagccggt gcacacctgg gagctgcctc gctagggtcg accgctcgcc gagactgcgt
     4621 cttggcagcc aaagttcgag tacgggcctg cgtgattgca gtctcggcga gaggacagga
     4681 gtaggttcgg tcccatgtca gaggtaatga attgggacga cgcttaccag gaacagggat
     4741 tcttcgaagg ccccccacca tggaatatcg gtgagccgca acccgaaatc gccgagttga
     4801 tccgcgccgg caagtttcgc agcgacgtgc tcgatgccgg ctgcggggtc gccgaagcgt
     4861 cgctcgctct ggcggcacaa gggcacacgg tcgtggggat cgacttgacg cccaccgcgg
     4921 ttgccgcggc cagcgaggcg gcgcaggagc gggggttgtc caacgcgacc ttcgtccaag
     4981 tcgacatcac gtcgttcacc ggctacgacg gccggttcaa cacgattctg gacagcacgc
     5041 tcttccactc gctgccgatc gaggcgcgcg acgactacct gcgttctgtg catcgcgcgg
     5101 cggcaccggg cgcgtcgtac tacatcctgg tgttcgcgac gggtgcattc cctgccgacg
     5161 ctgacttcgg gccccacgtc gtcaccgaag atgaacagcg gcaggcggtt tcaaagtact
     5221 gggacatcga cgaaatccgg ccggcatata tccacgccaa catgccttcg atgccgggca
     5281 tgcccgagcc gccaatgacg ttcgacgtcg acgagaaagg tcgtcagaag atgccggcct
     5341 tcctgctcac cgcgcacaaa gcgagctgag cgcccgcgcg aacgtcgtca aatcgtcctc
     5401 gctgacgtcc acatgcggcg agagccgcaa caccggggtg ctcagttcga acggcgcccg
     5461 gtcgggtccg gcgaccgttg tgacaatcgc gtgccgcgag atcagctcgg cccgaatctg
     5521 cgcgacgtcg gcgccgtcag tcggtgccag cgtggtgatc gcggtcggct cgtcgagcgg
     5581 ctcgacgacg cgccaccccg ccaggtcgct cagcaccgta cgggtcagcc ggccggcctc
     5641 cgccagacct gcgcgcacct tcgtggggcc gtcgtccata tgttctaaga tcgccgacga
     5701 aaaccccacg cgcgctgcga tattcgcttc gccgacttcc agttggcgca gcggcgggat
     5761 gtcggcccag tgctgcgata cccgggggcg caggctggcg gcacggtcgg cgcgcacgcc
     5821 gagcaccccg acgccgcgcg gaccggccag ccacttccgt gaggtgccgt agacagcgtc
     5881 ggcatccacc gcacagtcga cgtggccgag gccctgcgcc gcgtccacca ccagcggcac
     5941 gccgtactcg cggcaggttt cggcgatctc gcgcagcggc tgcacgatcc cgcggtggct
     6001 gggcaccacc gtcaggtgca caagcgacgg aatctcgcgg gcccactgcg cggcgaggtc
     6061 ctcgaccacc acgcgcccca gcgagtcgac cggcaacaag gccacgtcgt acccattgac
     6121 ttccatcacg ccaaggttcg gaccgaattc tcctggcaca caggcgatta cccggcgctc
     6181 gtccggccac gcacggagca gcagatcgag ggcattggtc gcgcccgtgg tgaacacgac
     6241 gtcgtcggca ctcattccgg tgagtgctgc gacgccaatg cggccaacag ccagaaccgc
     6301 gtccgcctcc tgcgcagcca cgtaaccgcc gacctcggct tcccgacggg cgcacgctgc
     6361 ggccgcgtcg atcgccgcga aactctggcg agagcaggca gcgctgtcga gatgcacgcc
     6421 cttgacagcc ggccgcgcgt cccgccagcg tgttcccgaa atcatttcgc cgcgagcgac
     6481 agcccgaagt caccgtccgg atcggtccac cattgagtca gcgtcaaccc ggccgcggcc
     6541 agctcggcgg tcaccgtctc ccggcggaat ttgcaggaga cctccgtcag catctcctcg
     6601 gccgcatcga agtcaacgtc gaggtcgagc gccccgacac gcacccgctg ggcaccgatg
     6661 gaccgcagcc acatctcgat tcgctcctcc tcgacgttcc agcgcgagac atgctcgaac
     6721 gcgtcgagat cgaaatcggc gtcgagttcc cggttcacca ccgcgagcac gttgcggttg
     6781 aacgccgctg tgatgccagc gctgtcgtcg tacgcgcgga ccagccggtc gacgtctttc
     6841 accaggtcgg tacccagcag caggctgtcg cccggtgcca tcaccgcggc gagggtcgcg
     6901 aggaactccg cacgcgggcc gggcgtcagg ttgccgatgg tggatccgag gaaggcaagt
     6961 aaacgcgttc caccgccggg aatttcggtg aggtgctcct cgaagtcgcc gcacaccgcg
     7021 tccacctcga tgccgggata ctccgcgacg atcgccccgc ccgcggtctg caacacgccg
     7081 gcgtcgacgt cgaacgggat gaaccggcgc agcgtgccgc agcggtgcag cgcgtcgagc
     7141 agcatcctgg tcttctctga cgtgccactg ccgagttcga cgagggtgtc ggcgcccgtc
     7201 acgtcggcga ttgacgccgc ctcggccctc aggatggcag cctcggcgcg ggtggggtag
     7261 tactccggca gccgggtgat ctgatcgaac aggtcgctgc cgacatcgtc gtaaaaccac
     7321 ttgggcggca acgacttcgg cgtctgtcgc agcccgtggt acacgtcgcg gcgtaacgcc
     7381 cggttggcgg agtcagccgc caggtggttg gccagcatga cgctcatcgc acacctttca
     7441 acggggtcaa cgtgacgtcg ccacgggaca cttcgaccag atggcggtcc ggcacatcct
     7501 gccacgcggg gtcgtcgtcg aacggctcgc tggccagcac gatcccgtcg tcaccatgaa
     7561 gcaccgacaa cgtatcgccc cacgtggtgg ccagcagcct tgacccgtcg gcggccaaaa
     7621 tgttcagtcg cgcattggga tcggcagtac ccacttcggt gatggtgtcg gccagcacgt
     7681 cgaggccgcg ctcgaagatc agcgccgcaa gtaatgcgct gtccaccacc gattcggctt
     7741 tcggtgacgg cgggagcacc gcgcggtcga ctaaaccatt gtgcgacaac agccatcggc
     7801 cgtcgctgaa cggcgccgac gccgacgcct ccaccggcat gccgacgctg gccgagcgca
     7861 ccgcggccac cacacaaccg ctgcgcagca ccggcgccac cgacgcgaac gacgcgtcgc
     7921 tccacagcgg ccccgcgctg cgccagcgcc gcggaacacc ttccgtgtca aagaatccca
     7981 cgccccagcc gtcggcgttc aatagtccgt gcttctggcg acgcggtgaa tacgactgca
     8041 cgccaagccc atacggcggg tcgagcacca gcgacgccag cgagtgcggc tcgccgagcc
     8101 agccgaggtg ccggcacatc agagggcgtc ccaggccagc cgcacgcccg agaagatctg
//
LOCUS       DASWBF010000194         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_743692,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000194 DASWBF010000000
VERSION     DASWBF010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743692"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VF874_06925"
     CDS             <1..165
                     /locus_tag="VF874_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX9175831.1"
                     /translation="TVVAVLLALAVTGWISARLGGANAGRAVLRVTVGGALAMATTFV
                     IGHFVGGAIG"
     gene            complement(182..317)
                     /locus_tag="VF874_06930"
                     /pseudo
     CDS             complement(182..317)
                     /locus_tag="VF874_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            372..548
                     /locus_tag="VF874_06935"
     CDS             372..548
                     /locus_tag="VF874_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175832.1"
                     /translation="MPRSVVAAEDHGLGVRYRLLETIRQYGEERLVDCGETQTLLIRH
                     AHFYAVLSVRAAFP"
     gene            complement(567..1925)
                     /locus_tag="VF874_06940"
     CDS             complement(567..1925)
                     /locus_tag="VF874_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175833.1"
                     /translation="MEIVLGVSMTPTTVRMVLVEGDKADGVTVDHDVFDITAIDGAAT
                     SSASDQVIAAVLGTRESAVAAGHHLVSTGVSWSERAEAAVLREALTARGIEDVMLVSE
                     LHAAGALAQAIGRAVGYRTTALMFIERDTATLSVVETADGSIVKVLSRSLHSDDAMAV
                     LTEMAASLEAHESRPEGMFVLGSGVDVTSVKSHLKDLVSLAISAPEQPELALARGAAL
                     AAANTPRFDASTVGLAYSQEPEDGTTAGAANPAGLAGAAAQLAAVGHDGASLDAADIT
                     PDSDDDQEGRKPFLLAGSLLTAIFAVGVVALVISLAVSIQPTVDQRPSPGDQAVVPSI
                     VPPAPPAAQDAPPVQNAPAVQPAPVVPPPAATTIQAPVPVVQQAPQAPAVVPVDPPAA
                     PAPVGAPPALFPAPIIQLPVPWLPPILQPPPYDPPAVKPPWYPIPQQPQPKWRKPGHG
                     HD"
     gene            complement(2334..2963)
                     /locus_tag="VF874_06945"
     CDS             complement(2334..2963)
                     /locus_tag="VF874_06945"
                     /inference="COORDINATES: protein motif:HMM:NF015336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175834.1"
                     /translation="MKNIAFSKDVAVHYTPDGATWKDRPLNFTSHFGDHDIFSGEVNE
                     QVLQFVIRYAVAGATFFDNNAGLDYHFASNLAMVGGNVVLNRATAKRGSQAGGGFVFT
                     TSWLEGQILVNNRSFAKYVGVRLSADNGHTFHDTQGFFAGSNADNGIFVGPGAEVWQF
                     KTPELNLDNSASEFRFAVFYQDQATGEVFWDNNFRQDYRVSKADGATIG"
BASE COUNT          526 a         1032 c         1058 g          551 t
ORIGIN      
        1 acggtcgttg ccgtcctgct cgccctggcc gtcacggggt ggatcagcgc acggctgggc
       61 ggcgccaacg ccggccgggc ggtcctgcgg gttaccgtcg gcggcgcgct ggccatggcg
      121 accacgttcg tcatcggcca cttcgttggc ggcgcgatcg gatagcacag gatcgaaggc
      181 cgtcggcctc cagctgacgg cactcgacgt gatacgcgca gcacagcgcg ccgacgtcga
      241 tgtcgtgcgg ggtacgaaga tccgcgacga cactcggaga accggggatc gccctgttcg
      301 agcagctcga agccgccaaa gctcacgacc cggtccagtg cgaggcggtc atagatctgg
      361 tcaccggtct ggtgccccgc tcggtggtgg ccgccgaaga ccacggcctg ggtgtccgct
      421 accgcctact ggagacgatc cgccagtacg gcgaggaacg gctcgtcgac tgcggcgaga
      481 cccagacgct gttgatccgc catgcccact tctatgccgt cctatcggtg cgggcggcgt
      541 tcccgtaggg cgcagccgct tgcgccctag tcatgtccgt gtccaggctt tcgccacttt
      601 ggttgtggct gctggggaat cgggtaccac ggcggcttta cggctggcgg gtcatagggg
      661 ggcggctgca gaattggtgg gagccacggc accggcaact ggatgatcgg cgccgggaat
      721 aacgccggcg gagcgccaac cggcgccgga gcagccggcg ggtcaaccgg cactacagcc
      781 ggagcctgcg gagcctgttg caccaccggc acgggcgcct ggatcgtcgt cgctgccggc
      841 ggtggcacca ccggcgcggg ctgcacagcg ggcgcattct gcacaggggg tgcatcctgc
      901 gcggccggcg gtgccggcgg aacgatgctc ggaacgacgg cctgatcgcc ggggctgggg
      961 cgctgatcga cggtcggctg gatgctgacc gccaacgaga tcaccagggc cacaacgccc
     1021 accgcgaaga tcgcggtcag taagctgccc gccagcaaga acggcttgcg gccttcttgg
     1081 tcgtcgtcgg agtccggcgt gatgtcggcg gcgtccaggg atgcgccgtc gtggccaacg
     1141 gcggcgagtt gggcggcggc gccggccaag cccgccgggt tggccgctcc tgcggttgtg
     1201 ccgtcttccg gttcttgcga ataggccagg cccacagtgg acgcatcgaa acggggtgtg
     1261 ttggccgccg ccagcgccgc gccgcgggcc agcgcgagct ccggctgctc gggtgcgctg
     1321 atagcaagcg acaccaggtc cttcaggtgc gacttcaccg aggtgacatc cacaccggag
     1381 cccagcacga acatgccctc cggccgcgac tcatgtgctt ccaggctggc ggccatctcg
     1441 gtcagcacgg ccatcgcgtc gtcgctgtgc aggctgcggc tgagcacctt cacgattgag
     1501 ccgtcggcgg tctcgacgac cgacagcgtc gcggtgtcgc gctcgatgaa catcagcgcc
     1561 gtcgtgcggt agcccaccgc gcggccgatg gcctgtgcga gggccccggc ggcgtgcagt
     1621 tcggacacca gcatcacgtc ttcgatgccg cgggcggtca gggcttctcg cagcacagcg
     1681 gcttcggcgc gctcactcca actcaccccg gtcgacacca gatggtggcc agcggcgacc
     1741 gcgctctctc tggtaccgag cactgcggcg atgacttgat cggatgcgct cgaagtagcc
     1801 gcaccgtcga tggcggtgat gtcgaagacg tcgtggtcga cggtgacacc gtcggccttg
     1861 tcgccttcaa ccagcaccat gcggaccgtc gtgggtgtca tcgacacacc gagtacgatc
     1921 tccaccgccc ctccaagcta tttgctatac agcttctcgg ccgtcagtcc ccacgccagc
     1981 cgtcaactag atgttccatc gccgctacca accttcggcg tacagcatcg ggaattagct
     2041 gatcttgatg aagttctgag gggaccgcat cggcgtgggc tcccgctcct gcgaaataaa
     2101 ccctactcgc ttaggacagt cctgacgtct gatccggggc gagggcatca cagtcagtcg
     2161 gcgcactgtc agccggggct ctggtagccg aggctgtagc gcgctgagcc gcaggcacgt
     2221 cggcagatcg agcagttgca tcggcacgga catcggcgga ctggtcggct cgccgcggtc
     2281 aagaagatga ccggcccccg gcgcaatagt agtgaaggga cggttctcgc gccttagccg
     2341 atcgtcgccc cgtcggcttt gctgacccgg taatcctgtc ggaagttgtt gtcccagaac
     2401 acctcgccgg tggcttgatc ttggtagaac acggcgaagc ggaattccga tgcggagttg
     2461 tcgaggttca gttccggcgt cttgaactgc cagacctccg cgccaggacc gacgaagatg
     2521 ccgttgtcgg cgttcgagcc cgcgaagaag ccctgagtgt catggaacgt gtggccgttg
     2581 tcggctgaga ggcggacacc gacgtacttg gcgaaggatc gattgttgac caggatctga
     2641 ccctcaagcc aggaagtggt gaacacgaag ccccctcccg cctgactgcc gcgtttcgcg
     2701 gttgccctgt tcagaaccac gtttccaccc accattgcga ggttggatgc gaagtggtag
     2761 tccagtccag cgttgttgtc gaagaaggtg gcgcccgcta ccgcgtagcg gatcacgaac
     2821 tgcagaacct gctcgttcac ctccccgctg aagatgtcat ggtcgccgaa atgcgacgtg
     2881 aagttcagcg gcctgtcttt ccacgtggcg ccgtcgggcg tgtagtgcac cgcaacgtcc
     2941 ttggagaacg cgatgttctt caccttcgcg gcaacggtgt gaacgtcccg gtgaatccgc
     3001 ctcccgagaa gaactcccgt cgcgctagct tgagcctcac gcctgcatcc atgtcctcag
     3061 ggtctcccac gaagtgcatc cgccggatga gaacgggatc atcacctaca acatggtcac
     3121 gggtctcgac ggcaagatga ggcccagcag acgggtcgac cgacgcc
//
LOCUS       DASWBF010000195         4665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_743816,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000195 DASWBF010000000
VERSION     DASWBF010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743816"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VF874_06950"
     CDS             <1..1939
                     /locus_tag="VF874_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9175835.1"
                     /translation="RSLPRAAAVLPRTLAAAGVPVRAPASATTPADHPAVRALLTVLA
                     AATGGLDGEQALALVTGPIGRIDPVSLRGLRRSLRRSENTGTPRRFEELLIDALTGEL
                     KVPAAYAPALRRVRAVLDAAAHAAEEDPNFALWQAWRRSGLQRRWLQARTRGGAAGAQ
                     ADRDLDAVTMLFDIADDYVAHTPGATVRGLVDHVSTLQLPPPEREPLIEPDTVAVVSA
                     HAALGREWDVVVIAGLQEELWPNTVPRGGVLGTQHLVDVLDGVADTASRRAPVLADER
                     RLLVSALGRARRKLLITAVDSDNDDATVPSVFVDELMQLAEAGDDVVPVVAPRVLAPA
                     ALVGRLRAVVCAPAGTVDEGRRRCAATQLARLAAAGVPGADPATWYGSTPVSTDEPLQ
                     ESDRLPVTLSPSTVQTIADCPLRWLLERHGGTNGRDMRSALGSVVHALIASDATTAAQ
                     LVAQLEKIWDALPFDSQWYSRNEFARHRAMLSAFCQWRAQTRHELTEVATEVDVDGVL
                     ASPDPDLPDVRVRGRVDRLERDGAGRLVIVDIKIGKSAASKDDAQRHAQLALYQLAVS
                     AGLVPDGAQPGGGRLVYLGKPGIVERDQAALTPETEREWQQALHRAAAATAGPTFLAR
                     INDSCGHCPVRSSCPAQAARS"
     gene            1936..4665
                     /locus_tag="VF874_06955"
                     /pseudo
     CDS             1936..4665
                     /locus_tag="VF874_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942786.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
BASE COUNT          671 a         1678 c         1646 g          670 t
ORIGIN      
        1 gcggtcgttg ccacgggcgg cggccgtact gccgcgcacg cttgccgctg ccggtgttcc
       61 ggtgcgcgcg ccggcgtcag caaccacgcc ggccgaccac cccgcggtgc gcgcgctgct
      121 caccgtgctg gccgccgcca ccggggggct cgacggcgag caagcgcttg cgttggtcac
      181 cgggccgatc gggcgtatcg acccggtgtc gctgcgcggt ctgcgccgca gcctgcggcg
      241 tagcgaaaac accggcaccc caaggagatt cgaggagcta ctcatcgacg cgctcaccgg
      301 cgaactgaaa gtgcccgccg catacgcgcc agctttgcgc cgggtgcggg cggttctcga
      361 cgcggcggcc catgcggccg aggaggaccc caatttcgcg ctgtggcagg cgtggaggcg
      421 gtctgggctg cagcgccgct ggctgcaggc caggacccgc ggcggtgccg ccggagcgca
      481 agccgataga gacctcgacg cggtcaccat gctgttcgac atcgccgacg actacgtcgc
      541 gcacaccccg ggggcgacgg tccgcggcct cgtcgaccac gtcagcacac tgcaactccc
      601 gccacccgaa cgcgagccgc tgatcgaacc ggacaccgtc gccgtcgtca gcgcgcacgc
      661 ggcgctcggc cgcgaatggg acgtggtggt catcgcgggt cttcaggaag aattgtggcc
      721 caacaccgtt ccgcgcggcg gcgtgctcgg cacacaacac ctcgtcgacg tcctcgacgg
      781 tgtcgccgac acagcgtcac ggcgtgcgcc agtgctggcc gacgagcgcc ggctgctcgt
      841 gtcggcgctc ggccgggccc gccggaagtt gctgatcacc gccgtcgaca gcgacaacga
      901 cgacgccacc gtgccatcgg tgttcgtcga cgagctgatg cagctggccg aagccggcga
      961 cgacgtggtg cccgtggtcg cgccgcgggt cctcgcaccc gcagctctgg tcggccggtt
     1021 gcgggccgtg gtgtgtgcgc ctgcgggcac tgtcgacgaa ggccgacggc gttgcgctgc
     1081 aactcaattg gcccgactcg cagcggcggg cgtgccgggt gccgacccgg cgacctggta
     1141 cggcagcaca ccggtcagca ccgatgagcc gctgcaggaa agcgaccggc tgccggtcac
     1201 actttcgccg tcgacagtgc agacaatcgc cgactgcccg ctgcgctggc tgctcgagcg
     1261 ccacggcggc accaacgggc gcgacatgcg ctcggcgctc ggttccgtcg tgcacgcgct
     1321 gattgccagt gacgccacga cggcggcgca gctcgtcgcc caacttgaaa aaatttggga
     1381 cgctttgccg ttcgactcgc aatggtattc gcgcaatgag ttcgcccggc accgggcgat
     1441 gctttcggcg ttctgccagt ggcgagctca gacccgccac gagctgaccg aggtcgccac
     1501 tgaagtcgac gtcgacggtg tgctggctag ccccgatccg gacctccccg acgtccgggt
     1561 gcgcggccgc gtcgaccgcc tggaacgtga cggcgccggc cggctggtga tcgtcgacat
     1621 caagatcggg aagagcgcgg ccagcaagga cgacgcccag cgccatgccc aacttgcgct
     1681 gtaccagctc gcggtgtcgg ccgggctcgt accagacggc gcgcagcccg gcggcggtcg
     1741 tctggtctac ctcggcaagc cgggcatcgt cgaacgcgac caggccgcgt tgacgccgga
     1801 gaccgagcgg gagtggcagc aggcgctgca ccgggcggcg gccgccaccg ccggccccac
     1861 atttctcgcc cgcatcaacg acagctgcgg ccactgcccg gtccgctcga gctgcccggc
     1921 acaagcggcc cgatcgtgac accgcgccat agccccgccg aattggccga cgcgctgggt
     1981 ctgcccgtgc ccactgacga gcaggcggct gtgatcgcct cgccgcctgg tccgcttgtc
     2041 gtgatcgccg gagccggtgc gggcaagacc gagacgatgg ccgcgcgcgt cgtgtggttg
     2101 gtcgccaacg gatacgccca tccgggcgag gtactggggt tgacgttcac ccgcaaggcc
     2161 gccgcacagt tgctgcgccg ggtccggtcc cggctgtccc gcctcgccgg tgcgggcctg
     2221 ggagatgacg ccgtcgacgc cgagccggcg accgtcagca cctaccacgc gttcgccggc
     2281 gccctgctcc gtgaccacgg actgctgttg ccccttgagc ccgacacccg gctgctcagc
     2341 gaaacggagc tgtggcagtt ggcttttcgc gtcgtcagcg accatccggg cgacctcgac
     2401 accgacaaga cccccgccgc ggtcacgtcg atggtgctgc ggttgtcgtc gtcgctggcc
     2461 gagcacctcg tcgacaccgc ccagcttcgt gacacgcacg tcgagctcga gcgcctcgtg
     2521 cacacgttgc ccgcaggccc gtaccagcgg gagcgcggtc ccagccagtg gctgctgcgc
     2581 atgctcgggg tgcaacacga gcgcacccaa ctggtgccgc tcgtcgacgc gttgcacgcc
     2641 cgaatgcgtg ccgagaaggt gatcgacttc ggtcagcaga tggcggcggc ggcgcggctg
     2701 gcggtggccc atcccgaggt cggcgagcag ctgcgccgcc gcttccgagt tgtgctgcta
     2761 gacgagtatc aggacaccgg tcacgcgcag cgcatcgcgt tgtcgtcgct gttcggctgc
     2821 ggggtcgacg acgagctggc gttgactgcg gtcggcgacc cgatccagtc gatctacgga
     2881 tggcggggcg cgtcggccac caatctgccg cggttcgcca ccgacttccc gcatgcggac
     2941 ggctccccgg cgccgaccct ggaactgcgc accagctggc gcaacccgcc gcggacgctg
     3001 catctggcca acgccgtttc ggccgacgcc cgccgccgct cggtcgcggt gcgcacgttg
     3061 cgggcccggc cgaacgcgcg ggccggcacg gttcgctgcg cactactcga tgacgtgcgg
     3121 gccgaacgtg attgggtggc ggatcatctg gcgcgccgcc accacgaatc caagagcgcc
     3181 ggaacactgc cacctaccgc tgcggtgctg gtgcggcgca acgcggacgc ggccccgatg
     3241 gccgatgccc tcgcggcgcg cggtgtgccc gtggaggtgg tgggactggc cgggttgctc
     3301 ggcgtcaccg aggtcgccga cgtggtggcg atgctgcgcc tggtggccga cccgagcgcg
     3361 gggtcggcag cgatgcgggt tctcaccggg ccccgctggc ggctcggcgg caaagacatc
     3421 gcggcgctgt ggcagcgggc gaaagacatc gaccaaggtg gcgcgccggc atcgctcgac
     3481 gagattgccg cgcaagccgc acccgacgcc gacaccgcct gtctcgccga cgcgatctgc
     3541 gaccccggct cgccggccgg ctagtcggag gaggggtatc ggcgcttcgt cgcgctgggt
     3601 cgcgaactga ccgcgctgcg cggccggctc gaccacccgc ttccggatct cgttgcggag
     3661 gtccgaagga cgctcggcgt cgacatcgaa agccgggcgg cacgcccggt cgacgcgggc
     3721 tggtcaggca gcgaacacct cgacgcattc gcggatgtgg ttgcggcgta tgcggaacgt
     3781 ccgaatgggt cggtgacggg attcctggcc tacctcgacg ccgcagcggt cgtcgagaac
     3841 ggtttggcac ccgccgacgt gacggtgtcc aaggatcgcg tgcaggtgat gaccgtgcac
     3901 gccgccaagg gactcgagtg gcagatcgtc ggcgtgccgc atctgaccgc gcgcatattt
     3961 ccgtccaccg cgtcgacgcg cacctggctg accgacgccg cggatctgcc cccgctactc
     4021 cgcggcgacc gctcggcgga cggcgtgctc ggagtgccgg tgctggacac ctccaccgtg
     4081 acgaaccgaa agcagttgtc ggacaccatc aatgcccatc gccgtcaact cgagcaacgg
     4141 cgcgtcgacg aggagcgtcg cctgctgtac gtggccatca cccgcgcgga ggacacgttg
     4201 ctgctgtccg ggcatcattg gggaccgtcg gccgccaagc ccagcggccc gtcggtgttc
     4261 ctcaccgaac tcaaggccat catcgacaga tcggccgccg acggcgatcc gtgcggcgtg
     4321 atcgaacact gggccccggc gccggccgac ggtgaccgaa acccgttgcg cgacgaggtc
     4381 gtcgaggccc agtggcccgc cgatccgcgc gggtcgcgcc gcgccgacgt cgaacgcggc
     4441 gccgcgctgg tggccgcggc cgccgacacc ccgcccgaaa ccggcgacga ccccgagggc
     4501 tgggctgccg acgtcgatgc gttgctcgcc gagcgcgcgc gaagtgtggc gccgaaacat
     4561 gttctcccga gagaactgtc ggtgagcagc ctcgtcgagg tgggccgcga tcccgttggc
     4621 gcaccgcaga ggttgatgcg ccgcctccca gtccgtccag atccg
//
LOCUS       DASWBF010000196         4616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_744272,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000196 DASWBF010000000
VERSION     DASWBF010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744272"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1102)
                     /locus_tag="VF874_06960"
     CDS             complement(101..1102)
                     /locus_tag="VF874_06960"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9175836.1"
                     /translation="MPPTINMGIPGAASGVAQRAAGKRVLLAEPRGYCAGVDRAVETV
                     ERALERHGAPVYVRHEIVHNRHVVDTLAKAGAVFVKETDEVPEGAIVVFSAHGVAPTV
                     HESATERQLQVIDATCPLVTKVHNEAKRFARDDYDILLIGHEGHEEVVGTAGEAPEHV
                     QLVDGPDAVDDVTVRDEDKVVWLSQTTLSVDETMETVQRLRERFPKLQDPPSDDICYA
                     TQNRQVAVKAMAPECELVIVVGSRNSSNSVRLVEVALGAGAHAAQLVDYADDIDPAWL
                     GGVTTIGVTSGASVPEVLVRGVLERLAELGFDVVQPVTTANETLVFALPREIRPPRA"
     gene            1209..1775
                     /locus_tag="VF874_06965"
     CDS             1209..1775
                     /locus_tag="VF874_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid droplet-associated protein"
                     /protein_id="HEX9175837.1"
                     /translation="MASAPYGVRLLVGAAATVVEETRKFPQTLLMYPMTLVSQAAHIF
                     MRFQQNVADLVIKGDETLDALFPPKDEQPEWATFDEDLDEVPPVTQLADERERRTEGR
                     FALYSADPPEPAETNSDNSGETAATEPPDIAVELDYDDLTLAQLRARLQSLSVANLDA
                     LLSYEEATKARAPFQTLLANRITRASAK"
     gene            1772..3004
                     /gene="xseA"
                     /locus_tag="VF874_06970"
     CDS             1772..3004
                     /gene="xseA"
                     /locus_tag="VF874_06970"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX9175838.1"
                     /translation="MSAPAPPNSAEHPWPVRAVATRVAKWIDRLGMVWVEGQLTEIKV
                     RPDAKTVFMVLRDPAADMSLTVTCPRDLVLNAPVRLAEGTQVIVCGKPTFYTGKGSFS
                     LRLTEIRAVGIGELLAHIERLRRLLDAEGLFDVRLKRPIPFLPAAVGLITGRASAAER
                     DVMTVAAGRWPAVRFEVRNAVVQGPNAVPQIVDALRSLDRDPDVDVIVLARGGGSVED
                     LLPFSDETLCRAIAACTTPVVSAVGHEPDNPLCDLVADLRAATPTDAAKRIVPDMAAE
                     RALLDDLCRRGARALRHWVIREERIVQQLRSRPVLAAPLQMLTARSEEIHRARTAARR
                     DINRLVSAESDRVGHLSARLATLGPAATLARGYAVVQTVPGHSVLRSVGDAPSGTRLR
                     IRVADGAVGAVSSGEDVS"
     gene            3001..3228
                     /locus_tag="VF874_06975"
     CDS             3001..3228
                     /locus_tag="VF874_06975"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX9175839.1"
                     /translation="MTNPEVRPVSQLGYEEARDELIEVVRVLEQGGLDLDSSLKLWER
                     GEELAKQCEEHLAGARKRVEDALAGREGESD"
     gene            3290..4375
                     /locus_tag="VF874_06980"
     CDS             3290..4375
                     /locus_tag="VF874_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9175840.1"
                     /translation="MADASLTTDLGKVMVTGGSGFVGCNLVAELLERGHWVRSFDRVP
                     SPMADHPRLETVVGDICDTEAVAAAVNGVDTIFHTAALIELMGGKSVTEEYRKRSFGV
                     NVEGTKNLVHAAQKAGVKRFVYTASNSVVIGGKAISGGDETLPYTDRFADLYTETKVA
                     AEKFVLSQNDVDGLLTCSIRPSGIWGRGDQTMFRKVFERVVTGQVKVLIGSKNARLDN
                     SYVHNLVHGFILARQHLVEGGAAPGQAYFINDDDPVNMFEFARRVVEACGQDWPRFRI
                     SGRLVHAIMSLWQRLHFRFNLPTPLLEPLAVERLYLDNYFSVAKARRDLGYEPLYTTE
                     KAVQECLPYYVDVYKEVEAEGKKPAKV"
BASE COUNT          754 a         1504 c         1589 g          769 t
ORIGIN      
        1 gtgcgctacc gcggcagtcc ggcgaacgct ccccgctcgc ggtcgccgga ggccgacagc
       61 tgggagtacg acagctgaaa gcgcgtgcgg cgctttggaa ttaggcccgc ggtgggcgga
      121 tttctcgcgg cagcgcgaac accagcgtct cgttggcggt ggtcacgggc tgcaccacgt
      181 cgaagcccag ctcggccagc cgctccaaca ctccgcgcac caacacctcg ggtaccgaag
      241 cacctgacgt gacgccgatg gtggtgacgc cgccgagcca cgccggatcg atgtcgtcgg
      301 cgtagtcgac cagctgggcg gcgtgcgcgc ccgcaccgag cgcaacctcg accagccgca
      361 ccgagttcga cgaattacgg gacccgacga cgatcaccag ctcgcactcc ggggccatcg
      421 ccttgaccgc cacctggcgg ttctgcgtgg cgtagcagat gtcgtcgcta ggcggatcct
      481 gcagcttcgg gaagcgttcc cgcagccgct gcaccgtctc catcgtctcg tcgaccgaca
      541 gcgtggtctg agacagccac accaccttgt cctcgtcgcg gacggtcacg tcatcgacgg
      601 cgtccgggcc gtccaccagc tggacatgct caggcgcctc gccggcggtg ccgaccactt
      661 cctcgtggcc ttcgtgcccg atcagcagaa tgtcgtagtc gtcgcgggcg aagcgcttgg
      721 cctcgttgtg caccttggtg accagcgggc aggtggcgtc gatcacctgc agctggcgct
      781 cggtggccga ctcgtgcacg gtcggcgcga cgccatgcgc ggagaacacc acgatcgcgc
      841 cctctggtac ctcgtcggtt tccttgacga acacggcccc cgctttcgcg agcgtgtcga
      901 cgacgtggcg gttgtgcacg atctcgtgcc gcacgtacac cggcgctccg tgcctttcca
      961 gcgcgcgctc gacggtctcg acggcccggt cgacgccggc gcagtagccg cgcggctcgg
     1021 cgagcagcac ccgcttgccg gcggcccgct gcgccacgcc gctggcggcg ccaggaatcc
     1081 ccatgttgat cgtcggcggc atagagccag ggtacggtcc ggcgccgccc ccgcgccaaa
     1141 ttaggactcc gcgccacttg ccggggctgg cttttgagtt gcgggagtgg tagggcaggc
     1201 tagtgcccat ggcgagtgca ccatatgggg tccggttgct ggtcggggcc gccgcgacgg
     1261 tcgtggagga aacgcgcaag ttcccgcaga cgttgctgat gtacccgatg acgctggtga
     1321 gccaggcggc ccacatcttc atgcggttcc agcagaacgt cgctgacctg gtgatcaagg
     1381 gcgacgagac cctcgacgcg ctcttcccgc cgaaggacga gcagccggag tgggccacat
     1441 tcgacgagga cctcgacgag gtcccgccgg tgacgcagtt ggccgacgag cgcgagcgga
     1501 ggacggaggg gcggttcgcg ttgtactcgg ccgatccgcc ggagcctgcc gaaacgaact
     1561 cggacaacag cggcgaaacc gcggccaccg agccgccgga catcgcggtc gagctcgact
     1621 acgacgacct cacactggcc caactgcggg cgcggctgca gtcgctgtcc gtcgccaacc
     1681 tggatgcgct gctgtcctac gaggaagcca ctaaagcccg tgcgccattc cagaccctgc
     1741 tggccaacag gatcacccgc gcgtccgcca agtgagcgca cccgcaccgc cgaactccgc
     1801 ggagcaccca tggccggtgc gggccgtcgc cacccgggtg gcgaagtgga tcgaccgcct
     1861 cgggatggtg tgggtcgagg ggcagctcac cgagatcaaa gtcaggccgg acgccaagac
     1921 ggtgttcatg gtgctgcgcg acccggccgc cgacatgtcg ctgacggtca cgtgcccgcg
     1981 cgacctggtg ctcaatgcgc ccgtccggct cgccgaaggc actcaggtga tcgtctgcgg
     2041 caagccgacg ttctacaccg gcaagggttc gttctcgttg cggctcacgg agattcgtgc
     2101 agtcgggatc ggcgagctgc tggcccacat cgagcggctg cgacgactcc tggacgccga
     2161 gggcctcttc gacgtgcggc tgaagcggcc catcccgttc ctgccggcgg ccgtcgggtt
     2221 gatcaccgga cgcgccagcg ccgccgagcg cgacgtgatg accgtggccg cggggcgctg
     2281 gccggcggtg cgtttcgagg tgcgcaacgc cgtggtccag ggtccgaacg cggtgccgca
     2341 gatcgtcgac gcgttgcgct cgctcgacag ggatccggac gtcgacgtaa tcgtgctcgc
     2401 ccgcggcggc ggcagcgtgg aggacctgct gccgttctcc gacgagacgc tgtgccgcgc
     2461 gatcgccgcg tgcaccacac cggtggtcag cgctgtcggc cacgagcccg acaatccatt
     2521 gtgcgacctc gtcgccgacc tgcgcgccgc caccccgacc gacgcggcca agcggatcgt
     2581 gccggacatg gccgccgagc gggccctgct cgacgatcta tgccgtcgtg gcgctcgggc
     2641 cttgcggcac tgggtcatcc gggaggagcg gatcgtgcag cagctgcgtt ctcggccggt
     2701 gctcgccgcg ccgctgcaga tgctaacggc ccgcagcgag gagatccacc gggcgcgcac
     2761 cgccgcccgt cgcgacatta atcgcctggt gagcgccgag tctgaccggg ttggacactt
     2821 gtcggcgcgg ctggccacat tgggaccggc ggcgacgctg gcccgcggct acgcggtggt
     2881 gcagaccgtg cccgggcatt ccgtgctgcg ctcagttggc gacgcacctt cgggcactcg
     2941 gctgcggatt cgggtcgccg acggcgccgt cggggccgtc agctccggcg aggacgtgtc
     3001 atgacgaacc cagaagttag acccgttagt cagttgggat acgaagaagc gcgcgacgag
     3061 ttgatcgaag tcgtccgtgt cctcgaacag ggcggactgg acttggattc gtcgctgaag
     3121 ctgtgggaaa gaggtgaaga gctggcgaaa cagtgtgagg agcacttagc cggcgcacgt
     3181 aaacgagtgg aggatgcgct cgcgggacgc gagggcgagt cggactgaga agttttttcg
     3241 ttcgcaacta atggtcgaat tggaacaggt ttcagttatg ctctcgggca tggctgacgc
     3301 ctcattgacg actgatctcg gcaaggtcat ggtcaccggc ggctccggct tcgtcgggtg
     3361 caatctcgtg gccgagctct tggaacgcgg gcattgggtg cgatcgttcg accgggtgcc
     3421 ctctccgatg gccgaccacc cgcgtcttga gacggtggtc ggcgacatct gcgacacgga
     3481 ggccgtggcc gccgccgtga acggcgtcga cacgatcttc cacaccgcgg cgctgatcga
     3541 gttgatgggc ggaaagtcgg tcaccgagga gtaccgcaag cgcagcttcg gcgtgaacgt
     3601 cgagggaacg aagaacctgg tgcacgccgc gcagaaggcc ggcgtcaaac ggttcgtcta
     3661 cacggccagc aacagcgtgg tgatcggcgg caaggcgatc tccggtggcg acgaaaccct
     3721 gccgtacaca gaccgattcg ccgacctgta caccgaaacc aaagtggcgg cagagaagtt
     3781 cgtgctgtcg cagaacgatg tcgatggctt gttgacctgc tcgatccggc cgagcggaat
     3841 ctggggccgc ggcgaccaga ccatgttccg caaggtgttc gagcgcgtcg tcaccggcca
     3901 ggtcaaggtg ctgatcggca gcaagaatgc gcgccttgac aattcgtatg tgcacaacct
     3961 ggtacacggg ttcatcctgg ctcgacagca cctcgtcgag ggtggcgccg cacccggcca
     4021 ggcgtacttc atcaacgacg acgacccggt gaacatgttc gagttcgctc ggcgcgtcgt
     4081 cgaggcgtgc ggtcaggatt ggccgcggtt ccggatttcc ggccggctgg ttcacgcgat
     4141 catgagcctc tggcagcggc tgcatttccg gttcaacctg ccgacgccac tgctggaacc
     4201 gcttgcggtg gaacggcttt acctcgacaa ctacttctcg gtggcgaagg cccgccgcga
     4261 cctcggttat gagccgctat acaccaccga gaaggcggtg caggaatgcc tgccgtacta
     4321 cgtcgacgtg tacaaagagg tcgaagctga aggcaagaag ccggcgaagg tctagtcacg
     4381 ccggcacggc ccgagtgtgc ggtttcgtgc gcgacgcgcc gaggcgggcg tatgaaagcg
     4441 cacaatcgga gcgctcagct gtgctgcacc gccttagcct gaatccgtgg atcagtgatt
     4501 tcatgccgtc gcgggggctg atttctgcac gattggggcg gttcggaatg gtgtgggcat
     4561 gaatgatccg ctggcctgta ccagcttttc cttgttgcga ggttccttgg gtcggg
//
LOCUS       DASWBF010000197        10359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_676981,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000197 DASWBF010000000
VERSION     DASWBF010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10359
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676981"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VF874_06985"
     CDS             <1..212
                     /locus_tag="VF874_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175841.1"
                     /translation="AESRRVEAVVHDSSDPSRIGVVAENSCPIADGADVDDFADFADF
                     ADQTIRSAIKLFAELTPAAHLKDAG"
     gene            complement(214..696)
                     /locus_tag="VF874_06990"
                     /pseudo
     CDS             complement(214..696)
                     /locus_tag="VF874_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
     gene            1128..2192
                     /locus_tag="VF874_06995"
     CDS             1128..2192
                     /locus_tag="VF874_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9175842.1"
                     /translation="MRIIDADGHVAEGASLAVEAMKRWPEHIRPRADARGLVIEDRNY
                     PEDSGPGAGCPTEHGLSTAVGINCRSAEGVLGDADRDHIDTMVLYPSLGLCTPTLEDP
                     EFAAGFARLYNQWIASYCATSNGRLRGIAVTPIEHGQVAIDVMREAKELGLVATHIPP
                     ALKTRNLDHADLDPFYSAAVELDMPLGIHGAPGIHLPKIGVDRFTNYIQVHCISFPFD
                     QMTAMTALVSGGVFDRYPDLRVAFLEAGVGWVPFFIDRLHEHFEKRGDWIERGWQRDP
                     QEYLQAGNIWTTCEPEEPILPGVVDVLGDDFIMFASDYPHWDGEWPESTKHLRTRPDI
                     GEDTRAKIGGLNAQRFYGLN"
     gene            complement(2177..2914)
                     /locus_tag="VF874_07000"
     CDS             complement(2177..2914)
                     /locus_tag="VF874_07000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175843.1"
                     /translation="MRTALITGGSGGIGKACGRKLAALGYDVVLTARREAPLRAAAEE
                     MGARYVVADASRPEGFEPAVAALAEIDVLVHAAGVLGGTYARKQTFEQWRTTMSANLD
                     SCFVVTSAALPRMKAGSRFVFISSSAAHEPMRARTAYSASKAGMNAFARALALEVDRD
                     GISVHVVTPGPVETDMLQDVPFEMHAIRATDVAEAVAWLDTVDPSVDLPEIRMHAVTR
                     GPWSREPVIPTEVERRAAAKPRTQLRP"
     gene            complement(2925..3734)
                     /locus_tag="VF874_07005"
     CDS             complement(2925..3734)
                     /locus_tag="VF874_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX9175844.1"
                     /translation="MTTHGIGLEVRRRLSARRTAEIIADELRRQIIDGELADGDLLPR
                     QDVLVEQFNVSLVSLREALRILETEGLVSVRRGNRGGAVVHAPAKASAAYMLGLLLQS
                     DYVPLGDLGAALQELEPMCAALAARRTDRGDELIPKLREINEAMAENIDDGARFTEIG
                     NQFHDEVVRGCGNHTMIAVVGSLETLWTSHLQQWADKTAARGEYPSMRRRRVALTTHN
                     KVTDAIEAGDGERARRLAARHLADTQTYFMTGDPEQRIVALSPQAMARRRH"
     gene            3790..6096
                     /locus_tag="VF874_07010"
                     /pseudo
     CDS             3790..6096
                     /locus_tag="VF874_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891252.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            6112..6822
                     /locus_tag="VF874_07015"
     CDS             6112..6822
                     /locus_tag="VF874_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9175845.1"
                     /translation="MSDQRRADPRPALSRARLLDAATSLLRSGGPSAVTVDAVTRAAN
                     VARATLYRHFPSGNDLLAAAFYSLIPPVPMPPDEGSLSERLTTLVQAWAESMAEAPAA
                     LSAISWLSLGSGIDQLPSERARVARDSVEIRTLRERIAQQYSAPFDAVFDSPQANAEL
                     GEVDREQAIALLLGPILLGKLSTLAEFDYRECARAAVEGFLATHAKKPGGSAPGQRDP
                     HLVEDVHVGDEAGVGDLR"
     gene            complement(6742..7980)
                     /locus_tag="VF874_07020"
     CDS             complement(6742..7980)
                     /locus_tag="VF874_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9175846.1"
                     /translation="MTTTASVDLSDFSLWRNGFPDELFARLRREQPIFHHELTPGVAK
                     VVQRDFWMTTKHRHAVRIHRDVDSFTAVDGPLIQVLDTFSSYPTIINMDPPELTKRRR
                     VIASAFTPKAIARLEDGIRSRAASMVDRLLANGGGDWIGDVADLLPMSVIGDIIGMPE
                     EDRPRIFECFDRILKAKSPKARLSEQEEADLYTTIFSYALELTAEKRRNPADDIWSTL
                     TSAVITGEDGEQLSLPANELEIFFFVLALAGSDTTKNALAAGLQAFVANPEQIERYRD
                     DEALRTTAVEEVLRWSTPVTYWTRTAKVDVEIDGHTIPKGDRVVSMLRSANRDEDVFA
                     DPFVFDIGRDPNPHVAFGGGGPHHCLGAMLARAEIRAVFDELLLRADRIRLGSPKVAY
                     PSLVTNMYIFDEMGISLTRR"
     gene            8083..9174
                     /locus_tag="VF874_07025"
     CDS             8083..9174
                     /locus_tag="VF874_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX9175847.1"
                     /translation="MDPSEREFGPSGISLSPYRFPTGWFVVGFASDLAAGQVKRAHYF
                     GEELVIFRTESGQINVLDAYCQHLGANMGVGGTVEGEHIVCPWHGWHWNGDGTNALIP
                     YSKIGCKNNVRVRTYPALEWCGFILVWHERHGRAPYWQPPVLPELETGEYYPLHPHSR
                     MLNRVKVHPQMIIENAADPYHVQYVHKADSPANTASFEVTGYHLHATVNANFGGGRAK
                     TWLTPNGPVDAKIIYDNYSLGLGIVRFPSELVATIQVTGQTPVDEDYTDYFYTQASIR
                     EPGEAGDKPTGRAARFLQLQQEVIKQDFFTWENMKYLEKPNLAPEEARDYAALRRWAH
                     RFYPGTEPSPTDFGYTADGAPDPAAAHTS"
     gene            9171..9560
                     /locus_tag="VF874_07030"
     CDS             9171..9560
                     /locus_tag="VF874_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9175848.1"
                     /translation="MTLTYQQQYVLDGLAERAAILTLDARHNRLYSAGDLAGWITTFR
                     HSGATYTRTGETFTDLRAAFDGGNGQRLVTVDHEISVDGVDAMQRCVALLFSGAELRS
                     TGTYSDRLVYERGGWYFTSRELEWDPA"
     gene            9616..10089
                     /locus_tag="VF874_07035"
     CDS             9616..10089
                     /locus_tag="VF874_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9175849.1"
                     /translation="MVGVRTEPRLAGTAVSAARIQHFAALVRDGNPAYWDNDFAVRVW
                     GGLVAPPALLMGLLIPPPWLPSGQPRPASIALRVPLPGTTFINAANDAEFLTPILEGD
                     HLSVVEEVVSVSPEKTTQLGVGHFVETCESYQRYDGTLVAISRNTLFRFTPGTPS"
     gene            10086..>10359
                     /locus_tag="VF874_07040"
     CDS             10086..>10359
                     /locus_tag="VF874_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9175850.1"
                     /translation="MTWDEITVPTDLPEVVDEIDYQRVVMNAGATWDYYPGHYDPAYA
                     RNDGHPTIFVNTMHVAGFVDRVATEWAGPYSRVVRRTMRLLGPIYAS"
BASE COUNT         1729 a         3423 c         3422 g         1785 t
ORIGIN      
        1 atgcggagag ccggcgtgtg gaagcggtcg tgcacgattc ctccgacccg agtcggatcg
       61 gcgtcgtcgc ggagaactcc tgtccgatcg ccgacggtgc cgacgtcgac gacttcgccg
      121 acttcgccga cttcgccgac cagacgatcc ggtccgcgat caagctcttc gccgagttga
      181 cgccagccgc gcacctcaaa gacgccggct gagtcagaac gctctttccg ggatgcgcgt
      241 gatgtcgtcg tcgagcttct cgacgatgcc gcgcagcgcg gtcagcttcg gcagcatgtt
      301 cgttgcgaag aacgtcgctg tcgcgatctt gccttggtag aatgcctcgt cactcggtga
      361 gccggccaac gcggcgcggg cgacgccggc ttgcacgagc agtcgccacc cgatgatcag
      421 gtcaccgacg gccaacagga atcgcaccga cgccaacccg actttgtaga tttccgtggg
      481 gttttcggcg gcggccatca gacatccggt cagtgtggct gtcatcgctt ggacatcgtt
      541 caacgcggtc tgaagcaggt cggcctgcgg ccgcaacgcc tcgtcgcagt tctcgatcgt
      601 gcgagtgagc tgggacgcga catgcgccaa cgcctcgccg cggtcgcgga cgatcttgcg
      661 gaaggtgaag tccagcgcct gaattgcggt ggtgcccagc gagatccccg acgccgagcg
      721 gccattgtgt cgaatgcgct tgggccgcaa tgcgcgttgg tgtcgatgtc ctgtttcgct
      781 ggcgcgagaa ctctctctgc taggcggcta cctttctgcc cgcctctgcg cgacagcttc
      841 tgatctgtgg tgaagggttg tgttgtcgct gatcgctgtc tcctcgttat ggccttgacc
      901 tggggaaacg ccgttgtcgc tccggggcgg gcaaatcgca ctatgcctcc gacgccggcg
      961 gggtaccggt gagctcgccg aatagacgcc gacacgtaaa cggttgcgac gcatgccgac
     1021 ccgaggaacg ccgcagaggt tcaagtttcg tggcgccacc gctcaccgag gcgcggggcc
     1081 actgcggccc gccgccggct atgtcgctat tgtcgtatct ttagcaaatg cggatcatcg
     1141 acgccgacgg acacgtcgcc gagggcgcat cgctcgcggt cgaagcgatg aagcgctggc
     1201 cggaacacat caggccacgt gctgatgccc gcggcttggt gatcgaagac cgcaactacc
     1261 cggaggacag cggcccgggc gcgggctgtc cgaccgagca tgggctgagc accgcggtcg
     1321 gcatcaactg caggtccgcc gagggcgttc tgggcgacgc cgaccgcgac cacatcgaca
     1381 cgatggtgct gtacccgagc ctcgggctgt gcaccccgac gctcgaggac cccgagttcg
     1441 ctgcggggtt cgcccggctc tacaaccagt ggatcgccag ctactgcgcg acgtcgaacg
     1501 gccggctgcg tggcatcgcc gtgaccccga tcgaacacgg gcaggtggcg atcgacgtca
     1561 tgcgcgaggc gaaggaactc ggcctcgtcg caacccacat cccgccggcg ctgaagacgc
     1621 gcaacctcga ccacgccgac ctggacccgt tctattccgc tgccgtcgag ctcgacatgc
     1681 cgctcggcat tcacggcgcg cccggcatcc atctgccgaa gatcggcgtc gaccgcttca
     1741 ccaattacat tcaggtgcac tgcatcagct tcccgttcga ccagatgacc gcgatgaccg
     1801 cgcttgtgtc cggcggcgtc ttcgacagat atccggatct gcgggtggcg ttcctcgaag
     1861 cgggtgtggg gtgggtcccg ttcttcatcg accgcctgca cgagcatttc gaaaagcgcg
     1921 gcgactggat cgagcgcggc tggcagcgcg atccgcagga gtatctgcag gccggcaata
     1981 tctggacgac ctgtgagccc gaggagccga tcctgccggg agtcgtcgac gtgctgggcg
     2041 acgatttcat catgttcgcc agcgactatc cgcattggga cggcgaatgg ccagagagca
     2101 caaagcattt gcgcacccgt cccgacatcg gcgaggacac gcgtgccaag atcggcggct
     2161 tgaacgcgca gcggttctac ggcctcaact gagtgcgcgg cttggccgcg gcgcggcgct
     2221 ccacttcggt gggtatcacc ggctcgcgcg accagggccc gcgcgtgacc gcatgcattc
     2281 tgatctcggg caggtcgacg gacgggtcga cagtgtcgag ccacgccact gcttccgcga
     2341 cgtcggtggc gcgtattgcg tgcatctcga acggcacgtc ttggagcatg tcggtctcga
     2401 cggggcccgg cgtgacgacg tggacgctga taccgtcgcg gtcgacctcg agcgcgaggg
     2461 cacgcgcgaa agcgttcatg cccgccttcg acgccgagta cgccgttcgc gcccgcatcg
     2521 gttcgtgtgc cgctgacgac gagatgaaca cgaagcgcga accggccttc atccgcggca
     2581 gcgcagccga ggtgactacg aaacacgagt cgaggttggc cgacatcgtc gtccgccact
     2641 gctcgaaagt ctgcttgcgc gcgtaggttc cgccaaggac gcccgccgcg tggacgagca
     2701 catcgatttc ggcaagcgca gccaccgccg gctcgaaccc ctccggccga gacgcgtcgg
     2761 cgacgacata tcgcgcaccc atttcctcgg cggccgcccg cagaggcgcc tcacgtcgag
     2821 cggtgagcac gacgtcgtag ccgagcgcgg cgagcttgcg gccacaggcc tttccgatgc
     2881 cgccgctgcc cccagtgatc aacgccgttc gcacgcagca cctttcagtg gcggcgccgg
     2941 gccatcgcct gcggcgacag cgcgacgatc cgctgctcgg gatcacccgt catgaaatag
     3001 gtttgggtgt cggcgagatg gcgcgccgca agtcggcgcg cgcgttcccc gtccccggct
     3061 tcgatcgcgt cggtgacctt gttgtgggtt gtcaacgcga cgcggcgcct gcgcatcgac
     3121 ggatactcgc cgcgtgctgc ggtcttgtcg gcccactgct gcaggtggct ggtccacagc
     3181 gtctcgaggc tgccgaccac cgcgatcatc gtgtggttgc cgcagccgcg cacaacctcg
     3241 tcgtggaact gattaccgat ctcggtgaag cgcgcgccgt cgtcgatgtt ctcggccatc
     3301 gcctcgttga tctcccggag cttggggatc agctcgtcgc cgcggtcggt acggcgcgcg
     3361 gccaaggccg cacacatcgg ttcgagttcc tgcagcgctg cgcccagatc gcccagcggc
     3421 acatagtcgc tttgcagtaa caaccccagc atgtaggcgg cgctggcctt cgccggtgcg
     3481 tgcaccaccg cgcctccgcg gttgccccgg cgtaccgaca ccaggccctc ggtttccaat
     3541 attcgcaacg cttctcgcaa cgacaccagg ctgacgttga actgttcgac gagcacgtcc
     3601 tgacgcggca gcaggtcgcc gtcggccagt tctccgtcga tgatctgcct gcgcagctcg
     3661 tcggcgatga tctcggcggt cctgcgcgcc gaaagccgac ggcgaacttc caagccaatt
     3721 ccgtgggtcg tcattgcttg gcaatgctaa cagctgacta tccgtcaaaa ctaactaaga
     3781 tagccgttcg tggcccgtac cccgctgaca gatctgcgcg tcgtcgagat cagtgaccgc
     3841 atcgccggcg actactgcgg caagttactt gtcgacgccg gcgctgacgt cgtgaaggcc
     3901 gaaccggccg gcggaagccc gatgcgtgcc tttactgtca ccggtgccga tcccgcggca
     3961 ggggccgatt cgccgctctt cagttacctc aacgccggca agcaaagcgt cacaacgctt
     4021 tccgatgacc tgctcaccga tgcggacgtc gtggtggtga ctgccaaccg ggctgcggcg
     4081 ttgagcaccg gtatcgatcc gcagcggctg ctgtccgtcg cgccgcggtg cgtggtcgtc
     4141 acggtgtccg atttcggatg gacgggaccg tgggccgaac gcgccgcgac ggagttcacg
     4201 ttgcaggcgt ggtcgggctg caccgggttc cgcggcgatc cggacggccc gccgatcgcg
     4261 atcggcggcg agctcgggga gtacatggcc ggcacgttcg ccgcattcgg cgcactggcg
     4321 attcaccgcc gtacccgcaa cggcggcccc ggcgagcatc tggacttgtc ggtgctggaa
     4381 gcgatgacac tgatgcagag ctgcgagtgg ctgcacgccc agctcatggg tgttccggcg
     4441 atcaagcgct ccgttgaggt gccgtcgatc gagccggcca aggacggcta cgtcggcatc
     4501 acgatggtca ccgggcagca gtggctcgac ttcgccgcga tggtcgaatg cccggaactc
     4561 accgaaatcc ctgagctgag attccaattg ggccgctggg agtaccgcga ctggatacgg
     4621 caacgcatcg gcggatggct tcgcgaacgc actgtcgcag agatcgtcga gctcggccag
     4681 ctgttccggt tgccgatcgc tgcgctgggc aacggcgcga cgatcctcga gatggagtac
     4741 atgcgcgagc gcggagtgtt cgtgcccaac ccgaccgggt tccgccagcc gcgtactccc
     4801 tggctgatgt cggacgcacg gcctgcaccg gtgcgcaaga ccccgtcgct ccggcagaac
     4861 gacgagatca actggccgcc aaggcaatca ggctcgccgc tgtcgtcgcc gcggctgcca
     4921 ctcgaggaca tccgaatcgt ggacctgacc gcattctggg cggggccgtc ggcgacgcat
     4981 tcgctcgcgg cgttcggcgc cgacgtcgtc aagatcgaat ccatccagcg tcccgacggc
     5041 attcgctact cgggcgggat gcgcagcgac gtcgaggact ggtgggaata cggctgggta
     5101 ttccagggcg tcaacaccaa caagcggtca gtgacgctgg atctataatc ccccgacggt
     5161 atcgagctct tcaaaacgct tgtggcgcag gctgacgcgg tcatcgagaa cttctcgccg
     5221 cgcgtgctgg accagttcgg gctgggcggt gacacgttgc tcgaactgaa tccccgcctc
     5281 gtggtggtcc ggatgccggc gttcgggctc gacggtccgt ggcgcgatcg cgtcggcttc
     5341 gccccgacga tggagcagat cgccggcctg gcgtgggtga ccgggatgcc cgacggcccg
     5401 cccgtcgcgc cgcggggtgc ctgtgacccg ctggcaggcg tgcacgcggc ctttgcgctg
     5461 ctggccgcgt tggagttcgt cgacgacacg ggccgcggac agctcgtcga gacgccgatg
     5521 atcgaaacgg tactcaacgt aacggcctcg cagccaatcg aattcgaggt cttcgggttg
     5581 acgctgcagc ggcgcggcaa tcgcggccac cccggagtga cgcagaatct ctaccagtgc
     5641 gccggcgacg acgactggat cgcggtaacc gtccgcacag acgcggagcg ggattcggtg
     5701 atgcagatga tcggacagtc ctcctgggcc gacgatgtcg accgccactt gcagcagtgg
     5761 ttcagtatgc agaagctcga cgacgtcgtg gagcgcctcg ccgacgccgg tgtgcccgcc
     5821 gcggcggtgg tgtcgccgtc ggcggtgacg gagaacccgc agcttcgcca ccgcggcttc
     5881 ttcgagcgtc tcgagcatcc gcgcaccggg cccggctggt acccctgtcc gccgttcgcg
     5941 cggctcgccg gcgtgcagcg gtggctgtcg cgccctgcgc cgacactggg ccagcacaac
     6001 agtgaggtgc tcaccgcgat gtgcgggctg accgacatcg acctggagcg cctcgccgcg
     6061 aacgcggtga tcggcacccg cccgaagggc ctgtaggtac cctggttcgc tgtgagtgac
     6121 cagcgacgcg ccgacccgcg accggcgctg tcgcgcgctc gccttctgga cgctgcgaca
     6181 tcactgctgc gctcgggcgg tcccagcgcc gtgaccgtcg acgcggtcac ccgggccgcc
     6241 aatgtcgcgc gcgccacctt gtatcgccac ttcccgagtg gaaacgactt actggcggcg
     6301 gctttctact ctttgatacc cccggttccg atgcctcctg acgagggttc gctctccgaa
     6361 cggttgacga cgctggtgca ggcctgggcc gagtcgatgg ccgaagcccc tgccgcgctg
     6421 tcggcgatct cctggttgtc gttgggaagc ggtatcgatc agctgcccag cgaacgcgct
     6481 cgggtcgcgc gggacagcgt ggagatccgc acgcttcgag aacggatcgc gcagcagtac
     6541 tcagcgccgt tcgatgcggt cttcgacagc ccgcaggcca acgcggagct cggtgaggtc
     6601 gaccgggagc aggcgatcgc cctgcttctc gggccgatct tgctgggcaa gctgtctacg
     6661 ctggccgaat tcgactaccg cgaatgtgct cgggcggctg tggagggctt tctcgctact
     6721 cacgccaaga agcccggcgg ttcagcgccg ggtcagcgag atccccatct cgtcgaagat
     6781 gtacatgttg gtgacgaggc tggggtaggc gaccttcggt gagcccagcc tgatccggtc
     6841 ggcgcgaagc agcagctcgt cgaacaccgc gcggatctcg gcgcgcgcaa gcatcgcgcc
     6901 gagacaatgg tgtgggccgc cgccgccgaa tgcgacgtgc gggttcgggt cacggccgat
     6961 gtcgaacacg aacggatcgg cgaagacgtc ttcgtcgcgg ttcgcggagc gcagcatcga
     7021 caccacccgg tcgcccttcg gaatcgtgtg cccgtcgatc tccacgtcga ccttcgccgt
     7081 gcgagtccag taggtgaccg gcgtcgacca ccgcaacacc tcctcgaccg cggtggtccg
     7141 cagcgcttcg tcgtcgcggt agcgctcgat ctgctccggg ttcgcgacga aggcctgcag
     7201 cccggcggcc aacgcgttct tcgtggtgtc gctgccggcc agggccagca cgaagaagaa
     7261 gatctccagc tcgttcgccg gcagcgacag ctgctcgccg tcctcgccgg tgatcactgc
     7321 cgacgtcagt gtgctccaga tgtcgtcggc ggggttgcgc cgcttctccg ccgtcagctc
     7381 gagtgcatag gagaagatcg tcgtgtaaag gtccgcctcc tcctgttcgg agaggcgcgc
     7441 cttgggcgac ttggccttca ggatccggtc gaagcactcg aagatgcgcg gacgatcctc
     7501 ctccggcatg ccgatgatgt caccgatcac actcatcggc agcaggtcgg cgacgtcgcc
     7561 gatccagtcg ccgccgccat tggcgagcag ccggtcgacc atcgacgcgg cacgcgagcg
     7621 gataccatcc tcgagcctcg cgatcgcctt cggagtgaat gcactcgcga tgaccctccg
     7681 tcgtttggtg agctccggcg gatccatatt gatgatggtc gggtacgacg agaacgtatc
     7741 caggacctgg atgagcggcc cgtcgaccgc ggtgaaggaa tccacatcgc gatgaatgcg
     7801 gactgcgtga cggtgcttgg tggtcatcca gaagtcgcgc tgcacgacct tggcaacacc
     7861 gggggtgagc tcgtggtgga agatcggttg ctcgcggcgg agtcgggcga acagttcgtc
     7921 gggaaaacca ttgcgccaca acgagaaatc tgacaaatca actgaagctg tcgtggtcat
     7981 atccggtcct cgtgcctgcc gccttgacct atcatcctaa aatactaaag ataggcgacg
     8041 gaggggcgtg tcggaacgga ggacgcagtg acggactcgg ccatggatcc gtcggaacgc
     8101 gagttcggcc cgagcgggat cagcttgtcg ccatacaggt ttccgacggg gtggttcgtc
     8161 gtcgggttcg cttcggacct cgccgccggg caggtgaagc gcgcccacta cttcggcgaa
     8221 gagctggtga tcttccgcac cgaatccgga cagatcaacg tgctcgacgc gtactgccag
     8281 cacctcggcg cgaacatggg cgtcggcggc accgtcgaag gcgagcacat cgtgtgtccg
     8341 tggcacggct ggcactggaa cggtgacggc accaacgcgc tgatcccgta cagcaagatc
     8401 ggctgcaaga acaacgtccg cgtccgcaca tatcccgcgc tggagtggtg cggcttcatt
     8461 ctcgtctggc acgagcgcca cggccgcgcg ccgtactggc agccgcccgt gctgcccgag
     8521 ctggagaccg gggagtacta cccgctgcac ccgcacagcc gcatgctcaa ccgggtcaag
     8581 gtgcacccgc agatgatcat cgagaacgcc gccgatccgt atcacgtgca gtacgtgcac
     8641 aaggccgaca gccccgcgaa caccgcgtcg ttcgaggtca ccggctacca cctgcacgcc
     8701 acggtgaacg cgaatttcgg tgggggccgg gcaaagacgt ggctgacacc caacgggccc
     8761 gtcgacgcca agatcatcta cgacaactac tcgctgggac tgggaatcgt gcgcttcccg
     8821 tccgagctgg tcgcgacgat tcaggtgacg ggtcaaacgc cggtcgacga ggactacacc
     8881 gactacttct acacccaggc gtcgattcga gagcccggcg aggccggcga caagccgacc
     8941 ggccgcgccg cccggttcct gcaactgcag caggaagtca tcaagcagga cttcttcacc
     9001 tgggagaaca tgaagtacct cgaaaagccg aatctggcac cggaagaggc gcgcgactac
     9061 gccgcgctgc gccggtgggc gcaccgcttt tatcccggga ctgagccgtc gccaaccgat
     9121 ttcgggtaca ccgccgacgg cgcgcccgac ccggcggcag cacacacgtc atgactctga
     9181 cgtatcaaca gcagtatgtg ctggacgggt tggccgagcg cgccgccatc ctcaccctgg
     9241 acgcgcgaca caaccggttg tattcggccg gagaccttgc cggatggatc accacgttcc
     9301 gccactcggg agcgacgtac acccggacgg gggagacatt caccgatctg cgcgccgcgt
     9361 tcgacggcgg caacgggcag cgtctggtca ccgtcgacca cgagatttcc gtcgacgggg
     9421 ttgacgcgat gcagcggtgc gtggcgctgc tgttcagcgg agccgaattg cggtccaccg
     9481 gaacctattc cgaccgtttg gtttacgaac gcggaggttg gtacttcacc tcgcgcgagc
     9541 tggagtggga cccggcgtga gccggtgagc atcagccacg agctcgtgtt cggcagctac
     9601 gaggatgcgc tgcggatggt gggtgtccgc accgaacctc gtcttgccgg caccgccgtg
     9661 agcgccgcca gaattcagca cttcgccgca ctggtgcgcg acggcaatcc ggcctactgg
     9721 gacaacgact tcgccgttcg ggtctggggc ggtctggtag ctccgccggc gctattgatg
     9781 ggcctgctga ttccaccgcc gtggctgccg tcgggacagc cacgccccgc gtcgatcgca
     9841 cttcgcgtgc cgctgccggg caccacgttc atcaacgccg ccaacgacgc cgagttcttg
     9901 acaccgattc tcgagggcga tcacctgagc gtcgtcgaag aggtggtgtc ggtgtcaccg
     9961 gagaagacca cgcagctagg ggtgggccat ttcgtcgaga cctgcgagag ctaccagcgc
    10021 tacgacggca cgttggtggc gatcagccga aacacgttgt tccgcttcac tcccgggacc
    10081 ccgtcatgac gtgggacgag atcacggtgc cgacggacct tccggaggtg gtcgacgaaa
    10141 tcgactatca gcgggtggtg atgaacgccg gcgcgacgtg ggattactac cccggccatt
    10201 acgacccggc gtacgcccgg aatgacgggc atccaacgat tttcgtcaac acgatgcacg
    10261 tcgcaggatt cgtcgaccgc gtcgcgaccg agtgggcggg cccgtacagc cgcgtggtgc
    10321 ggcgcacgat gcggctgctg ggtccgatct acgccagcg
//
LOCUS       DASWBF010000198         9484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_603851,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000198 DASWBF010000000
VERSION     DASWBF010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603851"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF874_07045"
     CDS             <1..243
                     /locus_tag="VF874_07045"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018159516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX9175851.1"
                     /translation="VGPYSPVSLGDYCAGSNHVLPTAGCARHSSGLSVQTFLRGIHIV
                     EYDEAALKDVSGHVITLAKAEDLPAHGEAMRRRFET"
     gene            240..1391
                     /locus_tag="VF874_07050"
     CDS             240..1391
                     /locus_tag="VF874_07050"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX9175852.1"
                     /translation="MNTPGQRITLDDLPLRDDLRGKSPYGAPQLEVPVRLNTNENPHP
                     PTPALVDDVARSVREAAVNLHRYPDRDAVALRTDLAAYLARQTGVHVGAENLWAANGS
                     NEVLQQLLQAFAGPGRSALGFVPSYSMHPIISDGTQTRWLEAHRADDFGLDTDAAVAA
                     ITEHRPDVVFVASPNNPSGQSVSLQDLCSLLDAMDTGILILDEAYGEFSSAPSAISLI
                     EEYPTKLVVTRTMSKAFAFAGGRLGYLIAAPAVIDAMLLVRLPYHLSALTQAAARAAL
                     RHADETLASVAALAAERERVTEALSRMGFRVIPSDANFVLFGEFADAPATWRRYLDAG
                     ILIRDVGIPGYLRVTTGLPDENDTFLNASANLAAQPPADARSTTTLGAS"
     gene            1388..2002
                     /gene="hisB"
                     /locus_tag="VF874_07055"
     CDS             1388..2002
                     /gene="hisB"
                     /locus_tag="VF874_07055"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX9175853.1"
                     /translation="MTENRRARVERTTKESDIVVELDLDGSGNVDVDTGVPFYDHMLH
                     SLGSHASFDLTVRTKGDVHIEGHHTIEDTAIVLGQALGQALGDKKGIRRFGDVFIPMD
                     ETLAHAAVDVSGRPYCVHTGEPDHLRHFTIAGSSVPYHTVVNRHVFESLAMNARIALH
                     VRTIYGRDPHHITEAQYKAVARALRQAVERDPRVTGVPSTKGAL"
     gene            1999..2619
                     /gene="hisH"
                     /locus_tag="VF874_07060"
     CDS             1999..2619
                     /gene="hisH"
                     /locus_tag="VF874_07060"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX9175854.1"
                     /translation="MTRKSVVVLDYGSGNLRSAQRALQRVGADVDVTADPAAATAADG
                     LVVPGVGAFEACMTGLRAIGGDKIIAERLSAGAPVLGVCVGMQILFDRGVEFGVESVG
                     CGQWPGAVTRLDAPVIPHMGWNVVDVGTGSRLFEGLDPDTRYYFVHSYAAQRWEGNAD
                     AVLTWATHHVPFLAAVEDGPLSATQFHPEKSGDAGAALLSNWVRGL"
     gene            2705..3439
                     /gene="priA"
                     /locus_tag="VF874_07065"
     CDS             2705..3439
                     /gene="priA"
                     /locus_tag="VF874_07065"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HEX9175855.1"
                     /translation="MPLILLPAVDVVDGRAVRLVQGQAGSETEYGSAFDAALGWQSDG
                     AEWIHLVDLDAAFGRGSNRELLAEVVGRLDVNVELSGGIRDDASLAAALATGCARVNL
                     GTAALENPQWCQKVIVEHGDKVAVGLDVQIVDGEHRLRGRGWETDGGDLWEVLERLDS
                     EGCSRFVVTDVTKDGTLTGPNIDLLANVAECTDAPVIASGGVSSLDDLRAIATLTDAG
                     VEGAIVGKALYAGRFTLPQALAAVGR"
     gene            3446..4258
                     /locus_tag="VF874_07070"
     CDS             3446..4258
                     /locus_tag="VF874_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEX9175856.1"
                     /translation="MPNAADLNALVAEAVTILDKAAVRFVEGHAADPAVRKKGNDFAT
                     EIDLAIEREVVDALTSATGIEVHGEEFGGADVDSPLVWVLDPIDGTFNYAAGLPLAAI
                     LLGLMVDGEPVAGLTWLPFTNERFTGVVGGSLRCNGVELPPVGPAALEDAVVGVGTFN
                     VEWRGRFPGRFRLAVVEHLSRVSSRLRIHGATGIDLAYTAAGILGGAISFGDHIWDHA
                     AGVALVRTAGGIVTDLAGNDWTPRSRAALAGVPQCHEQILEILRGVGDPEDY"
     gene            4258..5034
                     /gene="hisF"
                     /locus_tag="VF874_07075"
     CDS             4258..5034
                     /gene="hisF"
                     /locus_tag="VF874_07075"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX9175857.1"
                     /translation="MADVAVRVIPCLDVDDGRVVKGVNFANLRDAGDPVELAAVYDSE
                     GADELTFLDVTASSSGRATMLDVVRRSAEQVFIPLTVGGGVRTVADVDMLLRAGADKV
                     SVNTAAIARPELLEELSRRFGSQCIVLSVDARTVPEGSPPTPSGWEVTTHGGRRGTGI
                     DAIEWAARGAELGVGEILLNSMDFDGTKAGFDLPMLRAVRAAVTVPVIASGGAGAVEH
                     FAPAVVAGADAVLAASVFHFRELTIKEVKTAMAAEGIVVR"
     gene            5031..5378
                     /gene="hisI"
                     /locus_tag="VF874_07080"
     CDS             5031..5378
                     /gene="hisI"
                     /locus_tag="VF874_07080"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX9175858.1"
                     /translation="MTLNARVASRLKRDAHGLITAVAQERGTGKVLMVARMDDFALAR
                     TLETRQATYYSRSRRQHWVKGETSGHTQHVHSVRLDCDGDTVLLEVDQVGGACHTGDK
                     SCFDVDVLLADED"
     gene            5414..6346
                     /locus_tag="VF874_07085"
     CDS             5414..6346
                     /locus_tag="VF874_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9175859.1"
                     /translation="MSTGENSRAVIDMWAPIVPSAEIVEDLRSGFPAEQLQYLEVFTK
                     TRVSDEEFGTYVQSRRRTDEEILEALDEAGISYSLITGFDERSTCGVTFVHNESVAAL
                     VDRHPDRFVPFAGVDIMRGNSALGELEHWVTERGFRGLSLRPFMIGRPATDRAYFPFY
                     AKCVELGIPLSIHTSANWTRTRPSDLGHPRHIDDVACHFPDLTILMSHAGYPWVLEAC
                     LVAWKHPNVYLELGAHRPKYFAAPGAGWEPLLRFGQTTIQDKVVWGSGAFLINRPYPQ
                     LCDEMRTLPVEPTVLESWLWRNAARLLGLPANEK"
     gene            6598..7404
                     /locus_tag="VF874_07090"
     CDS             6598..7404
                     /locus_tag="VF874_07090"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175860.1"
                     /translation="MRPGPGVTVTEHHVAGDSAVRVLCHGTFPVPPNRPAVLWIHSGG
                     MVVGSPQFEAPTAGRLARELGAVVVSPDYRLAPENPFPAALDDCMTTLRWMHERADEL
                     GVDPTRIAVGGMSAGGGLAAAVAQRSYDEGLTLRAQVLVYPMLDDRTALREDHAGRGQ
                     FVFTPEANRFGWTAYLGREPRMSDAPEYAAPARREDISNLPTTWIGVGDFDILYDESV
                     EYADRLKACGVPCELVTVEGMYHGADGLAPKASSMQAFRQSFVNHLRAHL"
     gene            complement(7401..7865)
                     /locus_tag="VF874_07095"
     CDS             complement(7401..7865)
                     /locus_tag="VF874_07095"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9175861.1"
                     /translation="MKRGDLVDEFELPDQTGKDRKFSEFLADGPAVLFFYPAAGTPGC
                     TKEACHFRDLAKEFADVGATRVGISTDPVGKQAKFSENHSFDFPVLSDADGTVASSFG
                     VKRGLLGKLMPVKRTTFVIDIDRRVLEVISSEVNMNTHADKALEVLRARKSG"
     gene            7886..9412
                     /locus_tag="VF874_07100"
     CDS             7886..9412
                     /locus_tag="VF874_07100"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX9175862.1"
                     /translation="MTSTADLTVTTTREQFRALAAEHRFVPVTSKVLADSETPLSAYR
                     KLAANRPGTFLLESAENGRSWSRWSFIGAGAPSALTVRDGEAAWIGVTPQDAPRGGDP
                     LEALRATMDLLATQPLAGLPPLSGGLVGFFAYDMVRRLERLPSLAVDDLNLPDMLLLL
                     ATDMAAVDHHEGTITLIANAVNWNGTDERVDWAYDDAVARLDVMTQALGQPLPSTVAT
                     FGRPQPVHRAQRTLEEYSTIVERLVGDIEAGEAFQVVPSQRFEMDTNADPIDVYRMLR
                     VSNPSPYMYLLNIPKPDGAIDFSIVGSSPEALVTVKDGWAHTHPIAGTRWRGDTEEDD
                     LLLEKELLSDEKERAEHLMLVDLGRNDLGRVCVPGTVRVDDYSHIERYSHVMHLVSTV
                     TGQLAEGRTALDAVTACFPAGTLSGAPKVRAMELIEEVEKTRRGLYGGVVGYLDFAGN
                     ADFAIAIRTALMRDGTAYVQAGGGVVADSNGPYEYTEAANKAKAVLNAIAAAETLKAP
                     "
BASE COUNT         1555 a         3168 c         3195 g         1566 t
ORIGIN      
        1 gtcggcccgt actcgccggt gagccttggc gactactgcg cgggctccaa tcatgtgctg
       61 cccacggccg gatgcgcccg ccattccagc ggattgtcgg tgcagacatt cctgcgcggc
      121 atccacatcg tcgaatacga cgaggccgcg ctgaaagacg tgtccgggca tgtgatcacg
      181 ctggcgaagg ccgaggacct gccggcgcac ggcgaggcga tgcgccggag gttcgaaacg
      241 tgaacacccc cggacagcgc atcacgctcg acgacttgcc gctgcgcgac gacctgcgtg
      301 gcaagtcgcc ttatggcgca ccgcaactcg aggtgccggt gcggctgaac accaacgaga
      361 acccgcatcc gcccacgccg gcgttggtcg acgacgtcgc gcgctcggtg cgggaagccg
      421 ccgtgaacct gcaccgctat cccgaccggg acgcggtcgc gctgcgcacc gacctcgccg
      481 cctacctggc ccgccagacc ggcgtgcacg tcggcgccga aaacctttgg gccgccaacg
      541 gatccaacga agtgctgcaa cagctgctgc aggcgttcgc cgggcccggc cgcagcgcac
      601 tcggcttcgt gccgtcgtat tcgatgcatc cgatcatttc cgacggcacc cagacgcgtt
      661 ggctagaggc gcaccgtgcc gacgacttcg gcctggacac cgacgcggcc gtcgccgcga
      721 tcaccgagca ccgccccgat gtggtgttcg tcgccagccc gaacaacccg tcgggacaga
      781 gtgtttcgct gcaagaccta tgcagcctgc tcgacgccat ggacaccggc atcctgatcc
      841 tcgacgaggc gtacggcgag ttctcctcgg cgccaagcgc gatcagcctc atcgaggagt
      901 acccgacgaa gctggtcgtg acgcgcacga tgagcaaggc gttcgcgttc gcgggcggcc
      961 gactcggcta cctgatcgcc gcgcccgcgg tgatcgacgc gatgctgctg gtgcggctgc
     1021 cgtaccacct gtcggcgctc acccaggccg cggcacgggc ggcgctgcgg cacgcggacg
     1081 agacgttggc cagcgtcgcg gcactcgccg cggaacgtga gcgcgtcacc gaagccttga
     1141 gccgcatggg gtttcgggtc atccccagcg acgcgaactt cgtgctgttc ggtgagttcg
     1201 ccgacgcacc cgccacctgg cgtcgttacc tcgacgccgg cattctgatc cgcgacgtcg
     1261 gcattcccgg gtacctgcgt gtcactacag gtctgcccga cgagaacgac acattcttga
     1321 acgccagcgc gaatctggct gcccagccgc ccgcggacgc gaggagcaca acaactctag
     1381 gagcatcgtg accgagaacc gccgcgcccg cgttgagcgc acgaccaagg agtccgacat
     1441 tgtcgtcgag ctggacctcg acggcagcgg gaacgtggac gtcgacaccg gagtgccgtt
     1501 ctacgaccac atgttgcact cactcggcag ccacgccagc ttcgacctga cggtccgcac
     1561 caagggtgac gtgcacatcg agggacacca caccatcgaa gacaccgcga tcgtgctcgg
     1621 ccaggcgctg ggccaggcgc tgggcgacaa gaagggcatc cgccggttcg gcgacgtgtt
     1681 catcccgatg gacgagacgc tcgcgcatgc ggccgtcgac gtctcggggc ggccgtattg
     1741 cgtgcacacc ggcgagccgg accacctgcg gcacttcacg attgcagggt ccagcgttcc
     1801 gtaccacacc gtcgtcaacc ggcacgtgtt cgagtcgctg gcgatgaacg cgcgcatcgc
     1861 gctgcacgtg cgcacgatct acggccgcga cccgcaccac atcaccgaag ctcagtacaa
     1921 ggcggtggcg cgtgcgctgc gccaggccgt cgagcgcgat ccccgggtca ccggtgtgcc
     1981 gtccacgaaa ggcgccctgt gacaagaaag tccgtggtgg tgcttgacta cggctcgggc
     2041 aacctgcggt cggcccagcg cgcgttgcag cgcgtcggcg ccgacgtgga cgtcaccgca
     2101 gacccggccg cggccaccgc cgccgacggt ctggtggtgc cgggcgtcgg ggcgttcgag
     2161 gcgtgcatga cggggctgcg agcgatcggc ggagacaaga tcatcgccga acggctgtcc
     2221 gccggcgccc cggtgctcgg cgtgtgcgtg ggcatgcaga tcctgttcga ccgcggtgtc
     2281 gagttcggtg tcgaaagtgt cggctgcggc caatggcccg gcgctgtcac gcgattggac
     2341 gcgccggtga tcccgcacat ggggtggaac gtcgtcgacg tcggcaccgg cagccggctc
     2401 ttcgaggggc tcgaccccga cacgcggtac tacttcgtgc actcctacgc cgcacagcgc
     2461 tgggaaggca atgctgatgc ggtgctcacc tgggccaccc accacgtgcc gttcctggcc
     2521 gctgtcgagg acgggccgct gtcggcgacg cagttccatc ccgagaagag cggcgacgcg
     2581 ggagcggcgc tgctaagcaa ctgggtgcgg ggcctatgac actctccgcg aaacggtatt
     2641 ccacgcggag aatgtcgaga gaaggcatcg ccagttaccg tctcggcgta gaaacgggtg
     2701 aactatgccc ttgatcctgc tgcccgccgt cgacgtcgtc gacggccgcg ccgtgcgctt
     2761 ggtgcagggt caggccggca gcgaaaccga gtacggctcg gcgttcgacg ccgcgctcgg
     2821 ctggcaatcc gacggcgcgg aatggattca tctcgtggat ctcgacgctg cgttcggccg
     2881 cggctccaat cgggagttgc tggccgaggt ggtcggcagg ctcgacgtga acgtggaact
     2941 gtccggcggc atccgcgacg acgcgtcgct ggccgcggcg ctcgcaaccg gctgcgcccg
     3001 agtgaatctg ggcaccgcgg cgctggagaa cccgcaatgg tgccagaagg tcatcgtcga
     3061 gcacggcgac aaggtggcgg tcgggctcga cgtgcagatc gtcgacggag agcaccggct
     3121 gcgcggccgc ggatgggaaa ccgacggcgg cgacctctgg gaggtactgg aacgccttga
     3181 cagcgaaggc tgttcgcgct tcgtcgtcac cgacgtcacc aaggacggca cgttgaccgg
     3241 cccgaatatc gacctgctcg ccaacgtggc agagtgcacc gacgcgccgg tgatcgcctc
     3301 cggcggcgtg tccagtctcg acgacctgcg cgcgatcgcc acgctgaccg acgccggcgt
     3361 cgagggtgcg atcgtcggaa aggcgctcta cgcaggacgg ttcacgctgc cccaagcact
     3421 cgcagcggtg ggacggtaga ccgcgatgcc gaatgccgcc gacctcaacg cgctggtcgc
     3481 cgaggccgtc acgatcctcg acaaggcagc cgtacggttc gtcgagggcc acgccgccga
     3541 cccggcagtc cgcaagaagg gcaacgactt cgccaccgaa atcgatttag ccattgaacg
     3601 cgaggtggtc gatgcgctga cgtcggcgac cggaatcgaa gtccacggcg aagaattcgg
     3661 cggggcagac gtcgactcgc cgctggtgtg ggtgctcgat ccgatcgacg gcaccttcaa
     3721 ctacgcagca gggctgccgc tggcggcgat tctgctgggc ctcatggttg acggagagcc
     3781 ggtggctggg ctgacgtggc tgccgttcac caacgagcga ttcaccggcg tcgtgggtgg
     3841 ctcgttgcgc tgcaacggag ttgagttgcc gccggtgggg ccggcggcct tggaagacgc
     3901 ggtggtcggg gtcggcacct tcaacgtgga atggcgtggt cggtttcccg gccggttccg
     3961 gctggcggtg gtggagcatc tgagccgggt gtcgtcgcgg ctgcgcatcc acggcgccac
     4021 cggcatcgac ctcgcctaca ccgcggccgg aatcctcggt ggcgcaatca gtttcggtga
     4081 tcacatctgg gaccacgcgg cgggtgtggc gctggtgcga acggcgggcg gcatcgtcac
     4141 cgacctggcc ggcaacgact ggactccgcg atcaagggcg gcgctggccg gcgtgccgca
     4201 gtgccacgag cagatcctcg aaatcctgcg cggcgttggc gatcccgagg actactgatg
     4261 gcagacgtgg ctgtacgtgt catcccgtgc cttgacgtcg acgacggccg ggtggtcaag
     4321 ggcgtcaact tcgccaacct tcgcgacgcc ggcgacccgg tcgaattggc cgccgtctac
     4381 gactccgagg gcgccgacga gctgacgttc ctcgacgtca ccgcgtcgtc gtcgggccgg
     4441 gccacgatgc tcgacgtggt gcggcgctcc gccgagcagg tgttcatccc gctgaccgtc
     4501 ggcgggggag tgcgcacggt ggccgatgtc gacatgctgc tgcgcgcagg cgccgacaag
     4561 gtctccgtca acactgccgc gatcgcgcgt cccgagttgc ttgaggagct gtcccgccgg
     4621 ttcggatccc agtgcatcgt gttgtcggtc gacgcgcgca cggtacccga gggatcgccg
     4681 ccgacaccgt cgggctggga agtcacgacg cacggcggtc ggcgcggcac cggcatcgat
     4741 gcgattgagt gggcggctcg gggcgccgag ctcggcgtcg gtgaaatatt gctcaactca
     4801 atggatttcg acggcaccaa ggccggcttc gacctgccga tgctgcgcgc ggttcgggcg
     4861 gcggtcacgg ttccggtgat cgccagcggc ggcgcgggcg cggtggaaca cttcgcaccc
     4921 gcggttgtgg ccggagccga tgcggtgctg gccgcaagcg tttttcactt cagagagctg
     4981 acgatcaagg aggtcaagac ggcgatggca gcagaaggga ttgttgtgcg atgacactca
     5041 atgcaagagt cgcgtcgcgg ctcaaacgcg acgcgcacgg cctgatcacc gcggtggcgc
     5101 aggagcgcgg caccggcaag gtgctcatgg tggcgcggat ggacgacttc gcgctggccc
     5161 gcacgctgga aacccgccag gccacctatt actcgcggtc aaggcggcaa cactgggtca
     5221 agggcgaaac gtccgggcac acccagcacg tgcactcggt gcggctggac tgcgacggcg
     5281 acacggtgct gctcgaagtc gaccaggtcg gcggcgcctg ccacaccggg gataagagct
     5341 gcttcgacgt cgacgtgctg ctggccgacg aggattaagc tcctgcgccg ccgtccttca
     5401 gtaggttggc ggtatgtcca caggcgaaaa ctctcgggcc gtgatcgaca tgtgggcgcc
     5461 catcgttccg tccgctgaga tcgtcgagga tctacgttcc gggtttcccg ccgagcagct
     5521 tcaatacctg gaggtgttca ccaagacccg ggtgagcgac gaggaattcg ggacgtatgt
     5581 gcagtcacga cgccgcaccg acgaagagat tctggaggct ctcgacgagg cgggtatttc
     5641 ctatagcctg atcactggct ttgacgaacg ctcaacctgc ggagtgactt tcgtgcacaa
     5701 cgagtcagtg gcagccctcg tcgaccgtca tcccgatcgc tttgtcccat tcgccggcgt
     5761 cgacatcatg cgcgggaatt cggcgctggg cgaactggag cactgggtaa ccgaacgcgg
     5821 gtttcgcggg ctcagcctgc ggccgttcat gatcggcagg ccggccaccg accgcgcgta
     5881 ttttccgttc tacgcgaaat gcgtcgaact cggtattccg ctcagcatcc acacgtctgc
     5941 caactggaca cggacacggc ccagcgacct gggccacccg cgccacatcg acgacgtcgc
     6001 ctgccacttc ccggatctga cgatcctgat gagccacgct ggctacccat gggtgcttga
     6061 ggcgtgtctg gtcgcgtgga aacacccgaa cgtctacctc gagctcggtg cccaccgccc
     6121 caagtacttc gccgcccccg gcgcgggatg ggagccgctg cttcggttcg gccagacgac
     6181 gatccaggac aaggtggtgt ggggttccgg tgcgtttctc atcaatcgcc cttacccgca
     6241 gctgtgtgat gagatgcgca ccctcccggt ggagccgacg gtcctggaga gctggctatg
     6301 gcgcaacgcc gctcggctgc tcggtcttcc ggctaacgaa aaatagcgtt cactctgcgg
     6361 tgagggcggc attttgacca gaatcgcgcc ctgcacgcag actcgatgct tacatcgggc
     6421 atgaagaaag tcattgcgct tgcctgtctg ggagccgccg ctttcgtcgc ccggtgttat
     6481 ctgcagaaga ccatggcgga tgtccccacc gagcttcgcc gtccctttct gccactcacc
     6541 tccggttcct ttactccgcg gacgcttcca ctttggcgag ctgcatttcg tttgagaatg
     6601 agaccggggc cgggcgtcac ggtgacggag caccatgtgg ccggcgattc cgccgttcgg
     6661 gtgctttgtc acggcacctt cccagtcccg ccaaacagac cagcggtgct gtggattcat
     6721 tccggcggaa tggtcgtggg ctctccgcag ttcgaggcgc cgacggctgg gcgtctcgcg
     6781 cgcgagctcg gcgctgtcgt cgtctcaccc gactacaggc tggcgccgga aaaccctttc
     6841 ccggccgcac ttgacgactg catgacaaca ttgcggtgga tgcacgagcg cgcggacgag
     6901 ctcggcgtcg accccacccg gatcgctgtg ggcggaatga gcgctggggg cggcctcgcc
     6961 gcggccgtcg cgcagcgcag ctatgacgag ggactcactc ttcgtgctca ggtgctcgtc
     7021 tacccgatgc tcgacgaccg caccgccctt cgcgaggatc atgcggggcg cggccagttc
     7081 gttttcacgc ccgaggccaa tcggttcggg tggaccgcct acctggggcg ggaaccgcgg
     7141 atgtcggacg cgccggaata cgccgctcct gcgcggcgcg aggacatttc aaacctgccg
     7201 acaacgtgga tcggagtcgg cgatttcgac attctttatg acgagagcgt cgagtacgcc
     7261 gacagactga aggcgtgcgg ggtcccgtgt gaactggtca cagtcgaagg gatgtatcac
     7321 ggcgcggacg gcctcgcccc gaaggcgtcc tccatgcagg cgttccgcca gagtttcgtg
     7381 aaccacctgc gagcgcacct ctaacccgac ttccgcgccc gcagcacctc cagcgcctta
     7441 tcggcgtggg tgttcatgtt gacctcgctg gagatcacct cgagcaccct gcggtcgatg
     7501 tcgatgacga acgtcgtccg cttcaccggc atcaacttgc caagcaggcc gcgcttcaca
     7561 ccgaacgacg acgccactgt gccgtcggcg tcggagagca ccgggaagtc gaagctgtgg
     7621 ttctcactga acttcgcctg cttgcccacc gggtccgtac tgatgccgac ccgagttgcg
     7681 ccgacgtcgg cgaattcctt tgccagatcc cggaagtgac acgcttcctt ggtgcagccc
     7741 ggtgttcccg cggccgggta gaagaacagc accgccggac cgtcggccag gaactcgctg
     7801 aacttgcgat ccttgccggt ctgatcgggg agttcgaact cgtcgacgag gtcaccgcgt
     7861 ttcatgggtg tctacgctag accacgtgac atcgaccgcc gatctcaccg tgaccaccac
     7921 gcgcgagcag tttcgcgcgc tggcggccga gcaccggttc gtgcccgtca ccagcaaggt
     7981 cctcgccgac agcgagaccc ctctgtcggc gtaccggaag ctggccgcga accggcccgg
     8041 tacgttcctg ctcgaatccg ccgagaacgg ccggtcctgg tcgcggtggt cgttcatcgg
     8101 cgcaggtgcc ccgtcggcgc tgaccgtgcg cgacggcgaa gcggcgtgga tcggcgtcac
     8161 acctcaggac gcgccccgcg gcggtgaccc gctcgaggcg ctcagggcca cgatggacct
     8221 gctggccacc cagcccttgg cgggtctgcc gccgttgtcc ggcggcctgg tcgggttctt
     8281 cgcctacgac atggtgcgcc gtctggagcg gttgccgtcg ctcgcggtcg acgacctcaa
     8341 tctgcccgac atgctgctgt tgctggccac cgacatggcc gccgtggacc accacgaggg
     8401 cacgatcacg ctgatcgcca atgcggtgaa ctggaacggc accgacgaga gggtggactg
     8461 ggcgtacgac gacgcggtgg cccggctcga cgtgatgacg caggcgctgg gccagccgct
     8521 tccgtcgacc gtggcgacgt tcggccgccc gcaaccggtg caccgcgctc agcgcacgct
     8581 cgaggagtac tcgacgatcg tcgagcgtct ggtcggcgac atcgaggccg gtgaggcctt
     8641 ccaagtcgtc ccgtcgcagc ggttcgaaat ggacaccaac gccgatccca tcgacgtgta
     8701 ccgaatgctg cgggtgtcca accccagccc gtacatgtac ctgctgaaca ttccgaaacc
     8761 tgatggcgcc attgactttt cgatcgtcgg ctccagtccg gaggcactcg tcaccgtcaa
     8821 ggacggctgg gcccacacgc atccgatcgc gggcacccgc tggcgcggcg acaccgaaga
     8881 agacgacctg ctgctggaga aggagctgct ctccgacgag aaggagcgcg ccgagcacct
     8941 gatgctcgtg gacctcggcc gcaacgacct gggccgggtg tgcgtccccg gcacggtccg
     9001 cgtcgacgac tacagccaca tcgagcggta cagccacgtc atgcacctgg tctcgacggt
     9061 caccggccag ctcgccgagg gccggaccgc gctggacgcc gtcaccgcct gcttcccggc
     9121 cggcacgctg tccggcgccc cgaaggtgcg ggcgatggag ctcatcgaag aggtcgagaa
     9181 gacccggcgc ggcctgtacg gcggcgtggt cgggtacctc gacttcgccg gtaatgccga
     9241 cttcgcgatt gcgatccgca ccgcgctgat gcgcgacggc accgcgtacg ttcaggccgg
     9301 cggcggcgtc gtcgccgact ccaacggccc gtacgaatac actgaagcgg ccaacaaggc
     9361 caaggccgtg ctgaacgcga tcgcggctgc cgagaccctg aaggctccat gatccgcctc
     9421 gcccagctgc tgttggtgct ggcagcgatc ggattgtggg tcgcgtcgag actgccgtgg
     9481 gtgg
//
LOCUS       DASWBF010000199        10044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1133298,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000199 DASWBF010000000
VERSION     DASWBF010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10044
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133298"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VF874_07105"
     CDS             <1..274
                     /locus_tag="VF874_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175863.1"
                     /translation="AFENAEKATELVAIKAAGSEVAFLWRLTISTGDTGNRIEIISVM
                     TFDDDAKITSMRAFWSPADLTPLARACGSVPRHAAFSVQLRTLAQK"
     gene            complement(293..1039)
                     /locus_tag="VF874_07110"
     CDS             complement(293..1039)
                     /locus_tag="VF874_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX9175864.1"
                     /translation="MAVETAEDTAIVKPAQSPTTQAEDFPEAVADGVAHLLGKPRARG
                     WIHVYGAAVAIIAGAALVSVSWSVVSTRAGLATLIYTFTIVAMFAVSGAYHRLHWKSE
                     TARKWMKRADHSMIFVFIAGSYTPFALLALPQASGMVLLSIVWGGALAGVALKMLWPS
                     APRWVGVPLYLLLGWVAVWFIVPIMHGAGIAALVLLVVGGALYSIGGILYGLKWPDPW
                     PNVFGHHEFFHACTAVAAICHYIAMWFAVF"
     gene            1094..1882
                     /locus_tag="VF874_07115"
     CDS             1094..1882
                     /locus_tag="VF874_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX9175865.1"
                     /translation="MEIIPPRLKDPLYRLYEMRLRQGLERSKSELPRHIAVLCDGNRR
                     WARELGYDDVSYGYRVGAAKIAEMLRWCADAGIEMATVYLLSTENLQRDADELDALIE
                     IITDVVEEICAPPNRWSVQTVGDLELIGEKPARRLREAVESTPANGTFHVNVAVAYGG
                     RREIADAVRALLSKELANGATPEQMIEAVTVDAISENLYTSGQPDPDLVIRTSGEQRL
                     SGFLLWQSAYSEMWFTEAYWPAFRRVDFLRALRDYSARHRRYGM"
     gene            1897..3270
                     /locus_tag="VF874_07120"
     CDS             1897..3270
                     /locus_tag="VF874_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175866.1"
                     /translation="MAALSAVVFTMSWWLGLYLLARDPRKPVLVLAAIGLTSFAAVVA
                     LDAVRVTSAAHADLLSRIEIYLVAVPAIAWFAVLVELSRPCDSWRSRAGEALLIAIVA
                     AGALAGAVMAGSVDGPLRAGHWVMFGVISLSTLGAMLAAVVRRSQPGPVAGFVIVATL
                     FFALGNAILIIPLGLVPSWLALASTGFDVALLGLAVALWDAFDEGQALRADMLRSFAA
                     SGVLVVLFGGQALVGLAVVGPGGSGRTALTVLLFTSLALAIAIHVFSDTLAGLLDRVA
                     FWRSPALRADRAALRSTEAALPLRKAGPLDDIDDDAFTRLTRRALGHYGDLSKLVASP
                     LTALPEIDERLARRGVPDQPLERANELKALLSDGIARLKPRDGGDFGTTDEWRYYNAL
                     YFPYVVGVRAYAQNATASGLDPVARQAWQWFVTEVPQRSLHNWQNAAARLIAADLRGR
                     VPVTQAS"
     gene            complement(3392..3871)
                     /locus_tag="VF874_07125"
     CDS             complement(3392..3871)
                     /locus_tag="VF874_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9175867.1"
                     /translation="MRMSAKAEYAVRAMVELATVEEGVLLKTDELAQAQGIPPQFLVD
                     ILSALRTDRLVRSRRGRDGGYELGRPATDISVADVLRSIDGPLASVHDIGLGDLPYSG
                     ATAALTNVWRALRASMRSVLEQTSLADVAAGRLPDHVGKLAGDYLDQEHKRGHGTAS"
     gene            complement(3884..4642)
                     /locus_tag="VF874_07130"
     CDS             complement(3884..4642)
                     /locus_tag="VF874_07130"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX9175868.1"
                     /translation="MTDHDLAARLATEAGRLLLRVRDELAEASGSERKAAGDKRSHDF
                     LMEALSAERPGDAVLSEEGVDNPVRLSADRVWIVDPLDGTREFSELGRTDWAVHVALW
                     QSGELVAGAVALPAQGITLATPSVPAPPEAPSTPPDVAGQAAPVRIVVSRTRPPAIAL
                     DVRDRLGGTLVEMGSAGAKAASIAQGLADVYVHAGGQYEWDNAAPVAVARAAGLHASR
                     IDGSALEYNRADPLLPDLLICRPELADAVLAITS"
     gene            complement(4639..6498)
                     /gene="cysC"
                     /locus_tag="VF874_07135"
     CDS             complement(4639..6498)
                     /gene="cysC"
                     /locus_tag="VF874_07135"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX9175869.1"
                     /translation="MTSLLRIATAGSVDDGKSTLIGRLLYDSKAVMEDQLAAVERTSK
                     ERGNDYTDLALVTDGLRSEREQGITIDVAYRYFATPKRKFIIADTPGHTQYTRNMVTG
                     TSTAQLAIVLVDARQGLLEQSRRHAFLASLLGVQHIVLAVNKMDLIGWNQDRFEQIRD
                     EFHAFAARLNVHDVTTIPMSALLGDNVVSKSDKTPWYEGPALLSHLEEVYIAGDRNLV
                     DVRFPVQYVIRPQTLEHRDHRSYAGTVASGVMQVGDDIVVLPSAKTSRIVAIDSPTGP
                     VAEAFPPMAVSITLADDIDISRGDVIARINNQPRVTQEFDATVCWMADDTALEPGRDY
                     VIKQTTKTTRARVTALDYRLDVNTLHRDKSATALKLNELGRITLRTQVPLMLDEYTRN
                     ASTGSLILIDPTTNGTVAAGMVLRDVSARDAAVRASSPNTVKHQSLVTSSDRLSRGVT
                     VWFTGLSGSGKSSAAMLAERRLLEASRPAYVLDGDNLRHGLNADLGFSMADRAENLRR
                     LAHVATLLADCGQVVLVPAISPLAEHRELARRIHEEAGVPFFEVFCDTPLAECEERDP
                     KGLYAKARAGEITHFTGIDSPYQPPKNPDLRLTPDRTLEELAQQIVALVESQQ"
     gene            complement(6498..7424)
                     /gene="cysD"
                     /locus_tag="VF874_07140"
     CDS             complement(6498..7424)
                     /gene="cysD"
                     /locus_tag="VF874_07140"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX9175870.1"
                     /translation="MTAATSHLEAGHYELSHLRSLEAEAIHIIREVAAEFERPVLLFS
                     GGKDSIVMLHLALKAFRPARPPFPVMHVDTGHNFDEVIATRDELVAESGIRLVVASVQ
                     DDIDAGRVVDNGPSRNPLQTVTLLRAIRENKFDAAFGGARRDEEKARAKERVFSFRDE
                     FGQWDPKGQRPELWNLYNGRHHKGEHIRAFPLSNWTEYDIWSYIGAENITLPAIYYAH
                     RRPVFVRDGMLLAVHRFMQPTPAEEVFDASVRFRTVGDVTCTGCVESTAATVEDIIAE
                     TAVSRLTERGATRADDRISEAGMEDRKREGYF"
     gene            complement(7560..7784)
                     /locus_tag="VF874_07145"
     CDS             complement(7560..7784)
                     /locus_tag="VF874_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9175871.1"
                     /translation="MYVCLCLGITTEAVRDAVEGGASTSRQVAAACGAGSQCGRCRRT
                     VRAIIASEAKTDVIAKHHFWHVVRQLSGHH"
     gene            7910..8686
                     /locus_tag="VF874_07150"
     CDS             7910..8686
                     /locus_tag="VF874_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9175872.1"
                     /translation="MPDRGFERVALVIGGASGIGWAVAKGLAADGCRVTIADLNADAA
                     NQRAGELGVPHTGAAVDVTDEDAVAALFEDLGPLDIVVNTAGIGGVGRITELPLEQFR
                     SVVDVCLVGGFIVTKHAGRHLRDGGSLVSLTSLNARLAAPGMSAYCAAKAGLVMLTQV
                     AALELASRGIRVNAVSPGFVDTPLTEGVKLVPGTLEEYVENTPLGRVGTPQDVANAVL
                     FLVSDKASWMTGEVLDLNGGAHLKRYPDVLAHLEKMMSST"
     gene            8683..9498
                     /locus_tag="VF874_07155"
     CDS             8683..9498
                     /locus_tag="VF874_07155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175873.1"
                     /translation="MTHFSGKQALVTGAGSGIGAALSRALVAAGADVVCTDIDADAAE
                     RTAHVSTGPGTATSGRLDVTDAAAVQAAVDDVVSRTGRLDLMLNNAGIVWGGDTELLT
                     LDQWDTIIDVNIRGVVHGIAAAYPVMIRQGHGHIVNTASMAGLVPAGRLTSYAMTKHA
                     VVGLSLALRSEAAARGVDVLVVCPGAVETPILDKGAVGDFEGRSFFLTGVRSQTGYDP
                     DRLSRDVLRAIERNRGILVVPKIAYAGWLFVRLAPNLMQRASARMIARRRQLR"
     gene            9495..>10044
                     /locus_tag="VF874_07160"
     CDS             9495..>10044
                     /locus_tag="VF874_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEX9175874.1"
                     /translation="MSLPMPGRPTDVTSAWLSAALSAGGETVEVTDVDVVPVGTGQTG
                     ATYRIAATYAANPAGLPDTFVIKLPVQDDAVKGGVTLGYLSEVAFYARVADKMAVPTP
                     RCFYSDIANEGADFALLLEDMSPAVQGDQIVGATAEQAQLAVTGLAGLHGPSWCDPTW
                     LDLAEVAMPKPGDEPAIRGLGDI"
BASE COUNT         1617 a         3281 c         3423 g         1723 t
ORIGIN      
        1 ggcgttcgaa aacgcggaaa aggcaacgga attggtggcg atcaaggccg ccggcagcga
       61 agtcgcgttc ctgtggcgtc tgacgatcag caccggggac accggaaacc ggatcgagat
      121 aatcagcgtg atgacgttcg acgacgacgc gaagatcacc tcgatgcgcg cgttctggtc
      181 acccgccgac ctcacccccc tagcgcgagc gtgcgggtct gtaccccgac acgccgcatt
      241 ttctgtgcaa ctacgcacgc tcgcgcagaa gtgagggtgc gcaaccgcgc gtttagaaga
      301 ccgcaaacca catcgcgatg tagtggcaga tcgctgccac cgctgtgcat gcgtggaaga
      361 actcgtggtg gccgaacaca ttgggccacg gatcgggcca cttcaacccg tagaggatgc
      421 caccgatgct gtacagcgcg ccgccgacca cgagcagcac caacgccgcg atgccggcgc
      481 cgtgcatgat cggcacgatg aaccacaccg cgacccagcc cagcagcagg tacaacggca
      541 caccgaccca ccgcggcgcc gacggccaca gcatcttcag cgccactccg gcgagcgccc
      601 cgccccacac gatcgacaac agcaccatgc cactggcctg cggcagtgcc agcaacgcga
      661 acggggtgta gctgccggcg atgaacacga agatcatcga gtggtcggcg cgtttcatcc
      721 atttccgcgc ggtctcggac ttccagtgca ggcggtgata ggcgccgctc accgcgaaca
      781 tcgcgacgat cgtgaacgtg tagatcagcg tggccagccc ggcccgcgtc gacaccaccg
      841 accacgacac cgacaccagc gccgcgccgg cgatgatcgc gactgccgcg ccgtaaacgt
      901 ggatccatcc gcgcgctcgc ggcttcccca gtaagtgagc gacaccgtcg gcgacggcct
      961 cggggaagtc ctctgcttga gtggtcggtg actgcgccgg tttcacgatg gcggtgtctt
     1021 cagcggtttc aacggccatg gcacctccat tgccccaatc gattcctcga gtgccacagt
     1081 agtctggctt tccgtggaga tcattccgcc gcgcctcaaa gacccgttgt accggctcta
     1141 cgagatgcgc ctccggcagg ggctggagcg gtcgaagtcc gagctaccac gccacatcgc
     1201 ggtgctctgc gacggaaacc ggcgatgggc acgtgagctt ggttacgacg acgtcagcta
     1261 cggctaccgg gtgggggcgg cgaagatcgc cgagatgctg cgctggtgcg ccgatgccgg
     1321 gatcgagatg gccacggtgt acctgctgtc cacggagaac ctgcagcgcg acgccgacga
     1381 actcgacgcg ctgatcgaaa tcatcaccga cgtggtggag gagatctgcg cgccgcccaa
     1441 ccgctggagc gtgcagaccg tcggcgatct cgagctgatc ggcgagaagc cggcacggcg
     1501 gctgcgcgag gccgtcgaat cgacaccggc caacggtacc ttccacgtca acgtcgcggt
     1561 ggcctacggc ggccggcgag agatcgccga cgcagtgcgc gcgctgcttt ccaaggagtt
     1621 ggccaacggc gcgacgcccg agcagatgat cgaggccgtc accgtcgacg cgatctccga
     1681 gaacctctac acgtccggtc agcccgaccc cgatctggtg atccgcacct ccggtgagca
     1741 gcggctctcc ggatttctgc tgtggcagag cgcgtactcc gagatgtggt tcacggaggc
     1801 gtactggccg gcgtttcggc gggtggactt cctgcgcgcg ctgcgggact acagtgcccg
     1861 gcaccgccgc tacggcatgt gagggacaat tgagccatgg ccgcgctctc ggcagtggtg
     1921 ttcacgatga gctggtggct gggcctgtac ctgctggccc gcgacccgcg taaacccgtc
     1981 ctggtactgg ccgcgatcgg gctgacgagt ttcgcggccg tggttgcgct ggacgcggtg
     2041 cgggtgacca gcgcggcgca cgctgatctg ctcagccgca tcgagatcta cctcgtcgcg
     2101 gtgccggcga tcgcctggtt cgcggtgctc gtcgagctgt cgcggccgtg cgacagctgg
     2161 cgtagccgcg ccggggaggc gctgctgatc gcgatagtcg cggcaggcgc actggccggc
     2221 gcggtgatgg caggaagtgt cgacgggccg ttgcgggccg ggcactgggt gatgttcggg
     2281 gtcatctcgc tgtcgaccct cggcgcgatg ctggccgccg tggtgcgccg gtcgcagccg
     2341 gggccggtgg ccggtttcgt gatcgttgcg acgctgttct tcgcactcgg caacgcgatc
     2401 ttgatcatcc cgctaggcct ggtgcccagc tggctggcgc tggcgtcgac gggcttcgac
     2461 gtcgcgctgc tgggtctggc ggtggcgctt tgggatgcgt tcgacgaggg ccaggcgctg
     2521 cgcgccgaca tgttgcggtc gttcgccgcg agcggcgtgt tggtcgtgct gttcggtggg
     2581 caggcgctgg tcggcctcgc cgtcgtcggt cccgggggat ccggccgcac tgcgctgacc
     2641 gtgctgctgt tcaccagcct ggcgctggcc atcgcgatcc acgtgttctc ggataccctt
     2701 gccgggctgc tcgaccgggt ggcgttctgg cgatcgccgg cgctgagagc tgaccgggcc
     2761 gcgctgcgca gcaccgaggc ggcactgccg ctgcgcaaag ccggcccgct ggacgacatc
     2821 gacgacgatg cgttcacccg cctgacccgc cgcgcgctcg ggcactacgg cgacctatcc
     2881 aaactcgtgg ccagcccgct gaccgcgctc ccggagatcg acgagcggct agcccggcga
     2941 ggcgtgcccg accagccgct ggagcgcgcg aacgagctca aggcgcttct ttccgacggc
     3001 atcgcccggc tcaaaccacg cgacggcggc gacttcggca ccaccgacga atggcgctac
     3061 tacaacgcgc tctatttccc gtacgtggtc ggtgtgcgcg cgtacgcgca gaacgccacc
     3121 gcgtcgggcc tcgacccggt ggctcgtcag gcgtggcagt ggttcgtcac cgaggtccca
     3181 cagcgatcgc tgcacaactg gcagaacgcg gccgccaggc tgatcgcggc cgatctgcgc
     3241 ggccgggtac ccgtcacgca ggccagctag ctggcggcat ggcgtggcgg attccgcgac
     3301 actgaactca ctgcggcgac acgccgacga acccgccgag gtttcagttt cggcgcaaga
     3361 accgcaagga cttacggtct gcgcccggca gctagctggc ggtgccgtgg cctcgcttgt
     3421 gttcctggtc gaggtagtcg ccggcgagct tgccgacgtg gtcgggcaac cgtccggcgg
     3481 cgacgtcagc caggctggtc tgttcgagca ctgaacgcat gctggcgcgt aacgcccgcc
     3541 agacattggt caacgcggcc gtcgctccag aatagggcag atcgcccaac cctatgtcgt
     3601 gaacgctggc gagcggtccg tcgatcgagc gcagtacgtc ggcgacgctg atgtcggtcg
     3661 ctgggcggcc gagctcatag ccgccgtcgc ggccccgtcg gctgcggacg aggcggtccg
     3721 tgcgcaaggc ggacaggatg tcgacgagga actgcggcgg aattccttgc gcctgtgcca
     3781 gctcgtcggt cttgagcagg acgccttcct cgacggtcgc caactcgacc atggcccgca
     3841 cggcgtattc cgctttggcc gacatccgca tggcgcaatt ctttcagctg gtgatcgcaa
     3901 gcacggcgtc ggccaattcg ggccggcaga tcagcagatc gggcagcagc gggtctgcgc
     3961 ggttgtattc gagagcggag ccgtcgatgc gggaggcgtg taaaccggcg gcgcgcgcca
     4021 ccgccaccgg agcggcattg tcccactcgt attgaccgcc ggcgtggacg tacacgtcgg
     4081 ccaacccctg cgcgatcgac gcggctttgg ctcccgcaga acccatttcg acgagcgtgc
     4141 cgccgagacg gtcgcggacg tcgagcgcga tcgccggtgg cctggtgcgc gacaccacga
     4201 tccgcaccgg agccgcctgg ccggcgacat cgggcggtgt cgacggagcc tccggcggtg
     4261 cggggacgga cggcgtggcg agcgtaatcc cttgcgcggg aagggcaacc gctccggcga
     4321 ccagctcccc ggactgccac agcgccacat gcaccgccca gtcggtgcgg ccgagctcgg
     4381 agaactcccg cgtgccgtcg agcgggtcga cgatccacac ccggtccgcg gacagccgaa
     4441 ccgggttgtc gacgccttct tccgacagca ccgcgtcgcc gggacgctcc gccgaaagcg
     4501 cttccatcag gaagtcgtgg gatcgcttgt caccagctgc ttttcgctcg ctgccactag
     4561 cttcggcgag ctcgtcgcgc acccgcagca ggagtcggcc cgcctcggtg gccaggcggg
     4621 cggccaggtc gtgatcggtc attgctgcga ctcgaccagc gcgacaatct gctgcgccag
     4681 ctcttcgagc gtgcgatcgg gcgtcagccg gaggtcagga ttcttcggcg gttggtacgg
     4741 actgtcaatg ccagtgaagt gcgtgatctc accggcgcgc gccttcgcgt aaaggccctt
     4801 gggatcccgc tcttcgcatt cggccagcgg cgtgtcacag aacacctcga agaacggcac
     4861 cccggcctcc tcgtgaatcc tgcgtgcgag ctcgcggtgt tcggccagcg ggctgatcgc
     4921 gggcaccagc accacctgtc cgcagtcggc cagcagcgtt gcgacatggg cgagccgccg
     4981 caggttctcg gcgcggtcgg ccatgctgaa cccgaggtcc gcgttcaagc cgtgccgcag
     5041 gttgtcgccg tcgagcacgt acgcgggccg actcgcttcc aacagccggc gctcggcgag
     5101 catggcggcc gacgacttgc ccgagccgga taacccggtg aaccacaccg tgactccccg
     5161 tgacagccga tccgacgacg tcaccaagga ctggtgtttg acggtgttcg ggctcgatgc
     5221 gcgaaccgcg gcatcgcgcg cggacacgtc gcgcagcacc atgcccgcgg ccacagtgcc
     5281 attggtagtc gggtcgatca ggatgagcga tcccgtgctg gcgttgcgcg tgtactcgtc
     5341 gagcatcagc ggcacctgtg tgcgcaatgt gatgcggcca agttcgttga gcttcaatgc
     5401 cgttgcactc ttgtcgcggt gcagcgtatt gacatcgagc cggtagtcca gcgccgtgac
     5461 ccgtgcgcgc gtggtcttgg tggtctgctt gatgacgtag tcgcggccgg gctcgagcgc
     5521 tgtgtcgtcg gccatccaac acacggtcgc gtcgaactcc tgcgtcacgc gcggctggtt
     5581 gttgatccgc gcgatcacgt cgccgcggga gatgtctatg tcgtcggcca acgtaatcga
     5641 caccgccatc ggcggaaagg cctcggccac agggccggtc ggcgaatcga tcgcgacgat
     5701 ccggctggtc ttggcactgg gcagcaccac gatgtcgtca ccgacctgca ttacgccgct
     5761 ggcgacggtg ccggcgtagc tgcgatggtc acggtgttcg agcgtctgcg gccggatcac
     5821 gtactgaacc gggaacctga cgtccaccag gttgcggtca ccggcgatgt acacctcttc
     5881 cagatgcgac agcagcgcgg gtccctcgta ccacggcgtc ttgtcggact tgctgacgac
     5941 gttgtcaccg agcaacgccg acatcgggat cgtcgtgacg tcatgcacat tcagccgcgc
     6001 ggcgaacgcg tggaactcgt cacggatctg ctcgaaccgg tcctggttcc agccgatcag
     6061 gtccatcttg ttgacggcca gcacgatgtg ctgcacgccc agcagcgacg cgaggaacgc
     6121 gtgccggcgc gactgttcga gcagtccctg tcgggcgtcg acgagcacga tcgccagttg
     6181 cgcggtcgag gtgccggtca ccatgttgcg ggtgtactga gtgtggcccg gtgtgtcagc
     6241 gatgatgaat ttgcgcttgg gcgtggcgaa atagcgatac gcgacgtcga tcgtgatgcc
     6301 ctgctcgcgt tctgagcgaa gcccgtcggt caccagcgcc aggtcggtgt agtcgttacc
     6361 acgttccttg gacgtacgct cgacggccgc caactggtct tccatcacgg ctttggagtc
     6421 atatagcagc cggccgatca gcgtggactt gccgtcatcg acggacccag cggtggcgat
     6481 gcgcagcaga ctggtcatca gaagtagccc tcccgcttgc ggtcttccat tcccgcttcg
     6541 gaaatacggt cgtcggcgcg ggtggcaccc cgttcggtga gccgggacac cgcggtttcg
     6601 gcgatgatgt cttcgactgt ggcggcagtg gattcgacgc acccggtaca ggtgacgtcg
     6661 ccgacggtgc ggaaccgcac gctggcgtcg aaaacttctt cggcgggcgt cggctgcatg
     6721 aagcggtgca ccgccagcag catgccgtcg cggacgaaca ccggccggcg gtgcgcgtag
     6781 tagatcgcgg gcagcgtgat gttctcggcg ccgatgtagg accagatgtc gtattcggtc
     6841 cagttcgaca acgggaacgc gcggatgtgc tcacccttgt ggtgccggcc gttgtagagg
     6901 ttccacagtt ctgggcgttg gcctttcggg tcccactggc cgaactcgtc acggaacgag
     6961 aacacgcgct ccttggcacg ggccttttct tcgtcgcggc gggcaccgcc gaacgcggcg
     7021 tcgaatttgt tctcgcggat ggcgcgcagc agcgtgaccg tctgcagcgg gttgcgtgac
     7081 ggtccgttgt cgaccacgcg gccggcgtcg atgtcgtctt gcaccgaggc caccaccagc
     7141 cggatgccgg attcggcgac caactcgtcg cgtgtcgcga tcacttcgtc gaagttgtgt
     7201 ccggtgtcga cgtgcatgac ggggaagggc ggacgggcgg ggcggaaggc cttgagcgcc
     7261 aggtgcagca tcacgatcga gtctttgccg ccggagaaca gcagcaccgg ccgctcgaac
     7321 tccgctgcga cctcgcgaat gatgtgaatg gcctcggcct ccaacgacct caagtggctg
     7381 agctcgtagt gccccgcctc caggtgtgac gtcgcggcgg tcatgaagta cctcgtaatt
     7441 ctggtcaatt tggtcatgtt tatggggatt accaatatcg aaccaaacgg cgtcgcgagg
     7501 tgtcaagatc tcataggtcg cgaggtgtca agatctcata gaaagaaacg cagagcgcgt
     7561 cagtggtggc cgctgagctg cctgaccacg tgccagaagt ggtgcttcgc gatcacgtcg
     7621 gtcttggcct cggaggcgat gatcgccctg acggtccgtc ggcagcgacc acattgggag
     7681 cccgcgccgc atgcggcagc cacttgccgc gacgtcgacg cgccgccctc gaccgcgtcg
     7741 cgcacggctt ccgtggtgat gccgaggcac aagcacacgt acatcagctc aaccaccttg
     7801 ggtcgtaggt aaggctacgg taaccgaggc ggtgaacgtc tgtctacctt accgaaagtc
     7861 ttacggttac agtcgggaag ccgaatccgc tggttcagat ggaggcagat tgcccgacag
     7921 aggctttgag cgtgtggcgc tggtcatcgg cggtgcctca ggcatcggct gggcggtcgc
     7981 gaagggactg gccgcggacg gctgccgcgt caccatcgcc gacctcaacg ccgacgccgc
     8041 caaccagcga gccggtgagc tcggcgtacc gcataccggc gccgccgtcg acgtcaccga
     8101 cgaagacgcc gtggccgctc tgttcgagga cctagggccg ctcgacatcg tggtcaacac
     8161 ggccggaatc ggcggagtcg ggcgcatcac cgaattgcct ctcgagcagt ttcgttccgt
     8221 cgtggacgtc tgcctggtcg gcgggttcat cgtcaccaag cacgccggcc gtcatctccg
     8281 tgacggcgga tcgctggtgt cgttgacgtc gttgaacgcg cggctggctg cgccggggat
     8341 gagtgcctac tgcgccgcga aggcagggct ggtgatgttg actcaggtcg ccgcgctgga
     8401 gctcgcgtcg cgcggcatcc gggtcaacgc ggtgtcgccg gggttcgtgg atacgccgtt
     8461 gaccgaaggg gtcaagctgg ttcccgggac gctcgaggag tacgtcgaga acaccccgct
     8521 cgggcgcgtc ggcacgccgc aggacgtcgc gaatgcggtg ctgttcctgg tctcggacaa
     8581 agcctcgtgg atgaccggtg aggtgctcga cctcaacggc ggcgcccacc tcaagcgtta
     8641 tccggatgtg ttggcgcact tggaaaagat gatgtcgtcg acgtgaccca cttcagtgga
     8701 aagcaagcgc ttgtcaccgg cgcgggttcc ggtatcggcg cggcactttc gcgggcgctt
     8761 gtcgcggcgg gtgccgacgt ggtctgtacc gatatcgacg ccgacgcggc cgaacgcacc
     8821 gcccacgtgt caaccggacc gggcaccgca acctcgggtc gcctcgacgt caccgacgcc
     8881 gccgcggtac aggccgccgt cgacgacgtc gtgtcgcgca ctggccggct ggacttgatg
     8941 ctcaacaacg ccggcatcgt ctggggcggc gacaccgaac tgctcacgct ggaccagtgg
     9001 gacacgatca tcgacgtcaa catccgcggc gttgtgcacg gcatcgccgc cgcctacccg
     9061 gtgatgatcc gccaaggaca cggccacatc gtcaacaccg cgtcgatggc ggggttggtc
     9121 ccggcgggtc ggctcaccag ttatgcgatg accaagcacg ccgtggtcgg gctgtcgttg
     9181 gcgctccgtt ccgaggccgc cgcacgcggt gtcgacgtgc tggtggtctg ccccggggcc
     9241 gtggagacgc cgattctcga caaaggcgca gtgggagatt tcgaaggccg cagcttcttc
     9301 ctcaccggcg tgcgcagtca gaccgggtat gacccggacc gattgtcccg tgacgtgctg
     9361 cgcgccattg agcgaaaccg gggcatcctt gtcgtgccga aaattgccta cgcgggatgg
     9421 cttttcgttc ggttagcgcc gaatctcatg caacgcgcgt cggcccgcat gatcgcgcga
     9481 aggcggcaac tgcgatgagc ttgccaatgc ccgggcgccc caccgatgtc accagcgcgt
     9541 ggctctccgc cgcgctcagc gccggcggcg agaccgtaga ggtcacggat gtcgacgtcg
     9601 ttcctgtcgg caccggtcag accggcgcga cgtaccgcat cgcggcgacg tatgccgcca
     9661 atcccgcagg cttgcctgac acgttcgtga tcaagctgcc ggtgcaagac gacgctgtca
     9721 agggtggcgt gacactgggt tatctcagcg aggtcgcgtt ctacgccagg gtggcagaca
     9781 agatggctgt cccgacgccg aggtgcttct actccgacat cgccaatgag ggcgcggatt
     9841 tcgcgctgct gctcgaggac atgtcgccgg ccgtccaagg cgaccagatc gtcggcgcga
     9901 ctgccgagca ggcacagttg gcggtgacag ggctggccgg actgcacggg ccgagctggt
     9961 gcgacccgac gtggctggac ctcgccgagg tggcgatgcc caagcccggc gacgagccgg
    10021 cgatcagagg gctgggtgac atcg
//
LOCUS       DASWBF010000200         4900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_482279,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000200 DASWBF010000000
VERSION     DASWBF010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482279"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF874_07165"
     CDS             <1..187
                     /locus_tag="VF874_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9175875.1"
                     /translation="LGEISYEIFLLHVIVMEIAMVSVLRWPVYTGSMWGLFAVTLAMT
                     VPLAWVLHRLTRPRVRA"
     gene            complement(184..951)
                     /locus_tag="VF874_07170"
     CDS             complement(184..951)
                     /locus_tag="VF874_07170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175876.1"
                     /translation="MSTMGTVEIPVADIDAMPRGGPDASWLDRRLETGCREYLDRDDV
                     DERIKRSVVRALEWTGDVFKNHERFAYLVLDELADVADPKILELGAGHGGLSRKLLEQ
                     HPTARVTVTDIDATSVLDIAAGELGSHPRAVVHVADATEIDSPDGYFDLAVMALSFHH
                     LPPAQAAQVIFEGTRVAEKLMIIDLPRLPSLLHIVKLATFVPFLAIPFAHDGFISSMR
                     AYSPSALRALAAYADPRIKVDLRRQRLSPQVFIASRT"
     gene            990..2504
                     /gene="glpK"
                     /locus_tag="VF874_07175"
     CDS             990..2504
                     /gene="glpK"
                     /locus_tag="VF874_07175"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX9175877.1"
                     /translation="MADFVASIDQGTTSTRCMIFDQSGSEVGRHQLEHEQILPKAGWV
                     EHNPVEIWERTTSVLMSALNNTNLSDTDLAALGITNQRETTVVWNRKTGRPYHNAIVW
                     QDTRTDKIASALDRDDRGDVIRRKAGLPPATYFSGGKLQWILENVDGVRDAAENGDAV
                     FGTTDSWVVWNLTGGPRGGVHVTDVTNASRTMLMNLETLDWDDELLSLFGIPRAMLPE
                     IKPSSSPDPYGMTHPSGPLDNEVPLTGILGDQQAAMVGQVCLEQGEAKNTYGTGNFLL
                     LNTGEDIVRSENGLLTTVCYQFGDAKPVYALEGSIAVTGSAVQWLRDQLGIISGAAQS
                     EALARQVDDNGGVYFVPAFSGLFAPYWRSDARGAIVGLSRYNSNAHLARATLEAICYQ
                     SRDVADAMEADSGVHLEELKVDGGITANDLCMQIQADVLGVDVVRPVVAETTALGAAY
                     AAGLAVGFWSDPSELRANWQEDKRWSPNWSEDQREFAYAGWKKAVERTLDWVDV"
     gene            complement(2528..3238)
                     /locus_tag="VF874_07180"
     CDS             complement(2528..3238)
                     /locus_tag="VF874_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9175878.1"
                     /translation="MTEPFEGPHAGPPAEEFGFGPFDPRSRRAMHQARREARREVRGF
                     ARDHSRAQAAAHRGGPFGPGVEFGFGPGGPRPGHRGGPRGRGRGRGRSRRGDVRAAIL
                     ALLAERPMHGYEMIQEIGERSNGLWRPSPGSVYPTLQLLVDEGLIVAAESGGSKRLFE
                     LTDDGRETAAKAQTPPWTEIAEGADPNEVNLRSALEQLAGAVFQAAHAATPDQQKRIV
                     DIVNSARRDVYGVLGETD"
     gene            complement(3399..3917)
                     /locus_tag="VF874_07185"
     CDS             complement(3399..3917)
                     /locus_tag="VF874_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9175879.1"
                     /translation="MSLRHAALGLLSRGPASGYDLLKVFEASMANVWPATQSQLYAEL
                     NKLADAGLIEVSEVGPRGRKEYAITDAGRVELHRWLTSPQDDPVFRSAQLLRVFLLDE
                     MPCEQARKYLTALRAHADSEHKRYEQIRDSYDWSRGDKAFFARAALENGLRSTRMESE
                     WASWVLDELDKR"
     gene            3977..4545
                     /locus_tag="VF874_07190"
                     /pseudo
     CDS             3977..4545
                     /locus_tag="VF874_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
BASE COUNT          825 a         1592 c         1645 g          838 t
ORIGIN      
        1 gctgggcgag atctcctacg agatcttcct gctgcacgtg atcgtgatgg agatcgcgat
       61 ggtgtcggtg ctgcgctggc cggtgtacac cggctcgatg tggggcctgt tcgcggtgac
      121 gctggcgatg acggtcccgc tggcttgggt gctgcatcgc ctcacccggc cgcgggtgcg
      181 ggcctaggtt cggctcgcga tgaacacctg cgggctcaat cgctgcctgc gcaggtccac
      241 tttgatccgc gggtctgcgt aggcggcgag cgcccgcagc gcggacgggc tgtaggcgcg
      301 catcgagctg atgaaaccgt cgtgtgcgaa cgggattgcc aggaacggca cgaacgtcgc
      361 caatttcacg atatgcagca gcgacggcaa ccgaggcagg tcgatgatca tgagcttttc
      421 ggccacccgg gtgccttcga agatcacctg cgcggcctgc gccggcggca ggtggtggaa
      481 cgacaacgcc atcaccgcca ggtcgaaata gccgtcgggc gaatcgattt cggtcgcatc
      541 ggccacatgc accaccgcgc gcggatggct gccgagctcg cccgcggcga tgtccagcac
      601 cgacgtcgcg tcgatgtcgg tgacggtcac ccgggcagtc ggatgctgct cgagcagctt
      661 gcgggacagc ccgccgtggc ccgcgcccag ttcgaggatc ttcggatcgg ccacgtcggc
      721 gagctcgtcg agcaccaggt aggcgaaccg ctcatgattt ttgaacacgt cgccggtcca
      781 ctccagggca cgtaccacgc tgcgcttgat ccgctcgtcg acgtcgtcgc ggtcgaggta
      841 ttcgcgacag ccggtctcga ggcggcggtc cagccacgac gcatcgggcc cgcctcgcgg
      901 catagcgtcg atgtcggcga cggggatctc aacggttccc atagttgaca tcatggacga
      961 ctagccgcac gcacgacagg agcatctgag tggccgactt cgtcgcctcg attgaccagg
     1021 gcaccaccag cacccgctgc atgatcttcg atcagtccgg ttcggaggtg gggcgtcatc
     1081 agctggagca cgagcagatc ctgccgaagg ccggctgggt cgagcacaac cccgtcgaga
     1141 tctgggagcg caccacgtcg gtgctgatgt cggcgctgaa caacacgaac ctcagcgaca
     1201 ccgacctcgc cgcgctcggc atcacgaatc aacgcgagac cactgtcgtg tggaatcgca
     1261 agaccgggcg gccgtaccac aacgcgatcg tctggcagga cacccgcacg gacaagatcg
     1321 cgtcggcgtt ggaccgcgac gaccgcggcg acgtgatccg gcgcaaggcc gggctgccgc
     1381 cggcaacgta cttctcgggg ggcaagctgc aatggatcct cgagaacgtc gacggcgtcc
     1441 gggacgcagc ggagaacggt gacgcggtct tcggcacgac ggacagctgg gtcgtgtgga
     1501 acctgaccgg cggcccgcgc ggcggcgtgc acgtcaccga cgtcaccaac gccagccgca
     1561 cgatgctgat gaacctggag acgctggact gggacgacga gctgctgtcg ttgttcggga
     1621 ttccgcgagc gatgctgccc gagatcaagc cgtcgtcgtc gccggacccg tacgggatga
     1681 cgcatccgtc cgggccgctg gacaacgagg tgccgctgac cggcattctc ggtgaccagc
     1741 aggcggcgat ggtcggccag gtctgcctcg agcagggcga ggccaagaac acgtacggca
     1801 ccgggaattt cttgttgctc aataccggcg aggacatcgt ccgctcggag aacgggctgc
     1861 tgacgacggt gtgctaccag ttcggggacg ccaaaccggt gtacgcgctg gagggttcga
     1921 tcgcggtgac cggttcggcc gtgcagtggc tgcgcgacca actgggcatc atcagcggcg
     1981 ccgcgcagag cgaggcgctg gcccgtcagg tcgacgacaa cggcggcgtg tacttcgtgc
     2041 cggcgttctc ggggctgttc gcaccgtact ggcgctccga tgcgcgcggc gcgatcgtgg
     2101 ggctgtcccg gtacaactcg aatgcgcatt tggcccgcgc gacgctggag gcgatctgct
     2161 accagagccg cgacgtcgcc gacgcgatgg aagccgactc cggcgtccac ctcgaggaac
     2221 tgaaggtcga cggcggcatc accgccaacg acctgtgcat gcagatccag gccgacgtgc
     2281 tcggtgtcga cgtggtgcgg ccggtggtcg cggagacgac cgcgttgggc gcggcctatg
     2341 cggccgggct ggcggtgggc ttctggtcgg atccctctga gctgcgggcg aattggcagg
     2401 aggacaagcg ctggtcgccg aactggagcg aggaccagcg ggagtttgcg tacgcgggct
     2461 ggaagaaggc cgtcgaacgc acgctggact gggtcgacgt ctgactcacg ctcgcggcac
     2521 ggaattgtca gtcggtttcg ccgagcacgc cgtagacgtc gcgacgcgcg ctgttgacga
     2581 tgtcgacgat gcgcttctgc tggtcgggcg tggcggcgtg cgccgcttgg aagaccgcgc
     2641 cggcgagctg ctccaaggcc gaccgcaggt tgacctcgtt ggggtcagca ccctcggcga
     2701 tctccgtcca cggcggggtc tgcgccttgg cggcggtttc ccggccgtcg tcggtgagct
     2761 cgaaaagccg cttgctgccg cctgattcgg cggcaacgat cagtccctcg tcgacgagca
     2821 gttgcagcgt cgggtagacc gagccggggc tgggtcgcca caggccgttg ctgcgctcgc
     2881 cgatctcctg gatcatctcg tagccgtgca tcggtcgctc ggccagcagc gccaggatcg
     2941 ccgcgcggac atcgccgcgc cggctgcgac cgcgaccgcg gccgcgacca cgcggaccgc
     3001 cccggtggcc gggacgcgga ccgccaggtc cgaagccgaa ctcgacgccg ggcccgaacg
     3061 gaccgccgcg gtgcgcggcc gcctgcgcgc gggagtggtc tcgggcgaaa ccgcgcactt
     3121 cgcgtcgggc ttcgcgccgg gcctggtgca tggcgcggcg gctgcgcgga tcaaaggggc
     3181 cgaagccgaa ttcctcagcg ggtgggcccg cgtgcgggcc ctcgaatggt tcagtcatgt
     3241 gaatctcgtt tctcgttctg tgcgcgtgcg ccggatgcgc atcgcgatag ctcgacgata
     3301 tatcggaatc tatcgagatg caacactggg ccggtacccg acgttgactc tgcggccagg
     3361 gcgggaaagt tcgagtgggc tacgccctca gcgcagagtt aacgcttgtc gagttcgtcg
     3421 agcacccagc tcgcccactc cgactccatc cgggtggacc gcaggccgtt ttccagcgcc
     3481 gcgcgtgcga agaacgcctt gtcgccccgc gaccagtcgt aggagtcgcg gatctgctcg
     3541 tagcgcttgt gctcgctgtc ggcgtgggcg cgcagcgcgg tcaggtactt tcgtgcctgc
     3601 tcgcagggca tttcgtcgag caagaaaacc cgcaacagct gcgcgctgcg gaacaccggg
     3661 tcgtcctgtg gcgacgtcag ccagcggtgc agttcaacgc gtcccgcatc ggtgattgcg
     3721 tactccttgc gcccgcgcgg gccgacctct gacacctcga tcaggccggc gtccgccagc
     3781 ttgttcagct cggcgtacag ctggctctgg gtcgcgggcc acacgttggc catcgaagcc
     3841 tcgaacacct tgagcaggtc gtatccgctg gcgggaccac gggacagcag gccgagcgca
     3901 gcatgtcgca aactcacggc agcattttac cgtccgatat tgacatgtca ctagtggaat
     3961 gtcatagtcg acaaccatga cccgaaccgc agaaaccgtc gtcccatttt tccgtcgagg
     4021 caattacgcc ccggtggccg acgaactgac tgcattcgac ttactcgtcg agggttcgat
     4081 ccctcccgaa ctcaacggct ggtatctgcg caacggcccc aacccgcgac agtccacgca
     4141 gcactggttc accggcgacg gcatgattca cggagtgcgg ctggagaacg gccacgccgc
     4201 gtggtaccga aatcgctggg tacggaccga gagttttgac aacccgttcc ccgtctacaa
     4261 cgccgacggc acccgcaacc tgcactccag cgtcgcaaac acacacgtcg tcaggcatgc
     4321 cggcaagact ctcgcgctcg tcgagtcgtc gctgccgtac gagatcacca acgacctcca
     4381 gacgctgggc gcgcctacga cttcggcggc aagctcgtcg actcgatgac cgcccacccc
     4441 aagatctgcc caaccacagg cgaattgcac ttcttcggtt acggcaacat ctttgcgccg
     4501 catgtgacct atcaccgggc cgacgcgaac ggcgagctga ccatcatgtt ggttctcaaa
     4561 ctgcgtggcg gacgacgatg cctctacctg cggctttgcg aaattctcac cgaaatggcg
     4621 gatgagtttt tcgcatcttc gtggcggatc ctaccactga ggttttctca cttgggacgt
     4681 gagagtctgg tgtgactggt ggttctggtg tgtcgtgacc gatggacgtg gggtccttgc
     4741 tcgaggatcg gtttcgacca aaaacccttg tgccccaaga ggaccccacg tgttcaccta
     4801 ctctgccatc tgcgacgtgc ccgaacaaac gctgttgcat gtgaccgcgt tgctgcgcgc
     4861 gcaccgacac gagatcggca cccggaccgg acgacgcgcg
//
LOCUS       DASWBF010000201         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_18832, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000201 DASWBF010000000
VERSION     DASWBF010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18832"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1189)
                     /gene="serC"
                     /locus_tag="VF874_07195"
     CDS             complement(68..1189)
                     /gene="serC"
                     /locus_tag="VF874_07195"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX9175880.1"
                     /translation="MAEPTPTIPDSIKPNDGRFGSGPSKVRPEQLQALATTAAPLFGT
                     SHRQAPVKNLVGRVRSGLAELFSLPDGYEVVLGNGGATAFWDAAAFGLIDKRSLHLAY
                     GEFSSKFAAAVEKNPFIGDPIVDKAEPGSAPEPRSDPSVDAIAWAHNETSTGVAVPVR
                     RPAGSGSALILIDATSGAGGLPVDLADADAYYFAPQKNFASDGGLWLALLSPAALARI
                     ESIAASGRWAPDFLSLPIAVDNSRKDQTYNTPAIGTLVLLAEQLDWMLGNGGLDWAVK
                     RTADSSQRLYSWAEASSYATPFVTDPNLRSQVVGTIDFADEVDANAVAKVLRANGIVD
                     TEPYRKLGRNQLRVAMFAAVEPDDVSALTECVDWVVERL"
     gene            1353..>2182
                     /locus_tag="VF874_07200"
     CDS             1353..>2182
                     /locus_tag="VF874_07200"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9175881.1"
                     /translation="MNQGGEGHAANADGASGDIASIDELIDRAVGAVNCGDRVSASAL
                     AGQVLAVDRENPDAEDLLAAPGDVGEIRRLTILFADLVDSTRLSTRVEPETYRTLVGR
                     YRDMVLGVVDRFEGHVGSIKGDGVLAVFGHPKAHENDVRRAVLAALEITREVAWLGEQ
                     AKRRFGVDIAVRVGVHRGLVYLDTAQDDVYGLAANLAERVSGFAPPGTVVVSEAVAPL
                     VGEAFELQARPPAVVKGAEGPITYHLVLGECADAGRVGVGRGPLIGRDRELARLQRSW
                     A"
BASE COUNT          334 a          681 c          789 g          378 t
ORIGIN      
        1 cgcccccgcc cggacgacgg ccctggcaac cggcccatgc cccgtaaacc cctttccaaa
       61 cacgttctta gagacgttcg accacccagt cgacgcattc ggtgagcgcg ctgacgtcgt
      121 cgggctcgac ggccgcgaac atcgccaccc gcaactggtt gcggccgagt ttgcggtacg
      181 gctcggtgtc gacgatgccg ttggcccgca gcaccttggc gaccgcgttc gcgtcgacct
      241 cgtcggcgaa gtcgatggtg ccgaccacct gtgaccgcaa gttgggatcg gtgacgaacg
      301 gcgtcgcata cgacgatgcc tccgcccacg agtacagccg ctgcgacgag tcggcggtgc
      361 gcttgaccgc ccagtccagg ccgccgttgc ccaacatcca gtcaagctgc tcggccagca
      421 gcaccagcgt gccgatcgcc ggcgtgttgt acgtctggtc cttgcggctg ttgtccacgg
      481 cgatgggcaa cgacaggaag tccggcgccc agcgcccgga cgcggcgatc gactcgatgc
      541 gtgcaagcgc ggccgggctc agcagcgcca gccacaaccc gccatcgctg gcgaaattct
      601 tttgtggcgc aaagtaatac gcgtcggcgt cagccaggtc gaccggcagg ccacccgccc
      661 cggaggtggc gtcgatcagg atcagcgcgc tccccgagcc agcgggccgg cgcaccggga
      721 cggccacacc ggtcgaagtt tcgttgtgcg cccaggcgat ggcgtcgacc gacggatccg
      781 agcgcggctc cggtgcgctg cccggttcgg ccttgtcgac gattgggtcg ccgatgaacg
      841 gattcttctc caccgctgca gcgaacttgg aactgaactc gccataggcc aggtgcagcg
      901 accgcttgtc gatcaacccg aacgcggcgg cgtcccagaa tgcggtcgcg ccgccgttgc
      961 cgagcaccac ctcgtaaccg tcgggaagcg agaacagttc ggccaatccc gaccgcactc
     1021 gaccgaccag gttcttgacc ggggcctggc gatgggacgt gccgaacagc ggcgccgcgg
     1081 tggtggccag ggcctgcagc tgctcggggc gcaccttcga cgggcccgat ccgaatcggc
     1141 cgtcgttggg cttaatggag tcgggaatgg tgggcgtcgg ttcggccatg ggttccaggc
     1201 tagtgatccg ctgacggcgc cagatcagga ggttgccggc atagtgcgtg gagcctggcc
     1261 gtatccacgc ttcgccttcg gagttacccg ctgtcgctgg ttgtcgatgc gttcataacc
     1321 cgaatagagg ttcggaatga cacgatactg gcgtgaacca gggcggggag ggccacgcgg
     1381 caaacgctga cggcgcctct ggggacattg cctcgatcga cgagctgatt gatcgggcgg
     1441 ttggggctgt caactgcggt gatcgggtga gcgcgagtgc gttggcgggg caggttttgg
     1501 ccgtcgaccg cgagaatccc gacgccgagg acttgctggc cgctcccggg gatgtgggtg
     1561 agattcggcg gttgacgatt ctgttcgcgg atctggtgga ttcgacgcgc ttgtcgaccc
     1621 gggtcgagcc cgagacgtat cggacgctgg tgggtcgcta ccgcgacatg gtgctgggag
     1681 tcgtggaccg cttcgagggc catgtcggct cgataaaggg tgatggggtg ctggcggtgt
     1741 tcggtcatcc caaagcgcat gagaacgatg tccgccgggc ggtgctggcg gcactggaaa
     1801 tcacccgcga ggtggcctgg ctcggtgagc aagcgaagcg ccggttcggg gtggacatcg
     1861 ccgtgcgggt cggggtgcac cgcgggctgg tgtatctgga cactgcccag gacgacgtat
     1921 acgggttggc cgccaatttg gccgaacggg tgtccggttt tgccccgccg ggcaccgtag
     1981 tggtctccga ggcggtcgcg ccgctggttg gcgaggcgtt cgagctgcag gcccgcccgc
     2041 ccgcagtggt caagggtgcg gaggggccga tcacttacca cctggtgttg ggtgagtgcg
     2101 ccgacgcggg ccgtgtgggc gtgggccgtg gtccgcttat cggccgtgac cgtgagttgg
     2161 cgcggctgca gaggagctgg gc
//
LOCUS       DASWBF010000202         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_604020,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000202 DASWBF010000000
VERSION     DASWBF010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604020"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..323
                     /locus_tag="VF874_07205"
                     /pseudo
     CDS             198..323
                     /locus_tag="VF874_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739563.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
     gene            complement(316..1410)
                     /locus_tag="VF874_07210"
     CDS             complement(316..1410)
                     /locus_tag="VF874_07210"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX9175882.1"
                     /translation="MDEHPTIGVEEEFLLVDNDTGEPVAANQAVAAQAEARGVQLQLE
                     LTSCQVETTSRVVATSDELRVELTRLRRAAADAASACGARLLAVALPPSVPHHFPITD
                     TPRYRKIGENFGMIAHEQGICGCHVHVAVPNREAAVQVSNRLRPWLPLLLALTANSAI
                     YRNAETGYASWRSILWARWPSAGPPPFFESVEDYYATVRMLMDTGAVLDDGMVYWDVR
                     PSSRFPTIEVRVADVPSTVAETVLLATLIRAAVMTALDEFERGGAPPRVAAETLRAAY
                     WKAARDGLDGRAIDLPGGLRAVPTRDLLVEFAAHVRPALDKLGEYRRVSDEVARLAGE
                     GNGAMRQVRAWRRRGNAADVIAEAAAATLR"
     gene            1583..1849
                     /locus_tag="VF874_07215"
     CDS             1583..1849
                     /locus_tag="VF874_07215"
                     /inference="COORDINATES: protein motif:HMM:NF025590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEX9175883.1"
                     /translation="MTLLVGTVGCSNPPKTVTKEDVANQIKQKKWDEAGNKPESVSCP
                     IDLRPVVGAKVVCEMTINGQAVFVDVTVSGIDGDNVQLDMRGHR"
     gene            1878..>2155
                     /locus_tag="VF874_07220"
     CDS             1878..>2155
                     /locus_tag="VF874_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175884.1"
                     /translation="MMGAEQQSRKLGDPVARLLRLPVSTKEFIEKIVTGSVNESGRRT
                     LHVLFTTWDLAGGGPFAASAVASTGMAKTVDIVEKSLTVPVFSPLLKK"
BASE COUNT          398 a          715 c          676 g          366 t
ORIGIN      
        1 ttcggcagca tccgacagca ctttcggccc cgtcgtcatc tgatcagcgc gcccgaatgg
       61 cgaaccgaga tgaccaaccg cttcgcggtc tggaaccaga tcattaccgc cgcagcggct
      121 tgaaagacgc agtcagccac cctcttttgc cagccggtcc actcacatcg actgcgccac
      181 aagcattccc aaataacttg acgatgccca ctgctgtgct gcccaacctc gaaaacgcca
      241 ccacgctggc gttcacggtc gagccgggca ccggctcggt caagccgacc ggtcaggtgg
      301 tcgccgagtt gccgctcacc tgagcgtcgc cgccgcggcc tcggcgatga cgtcggcggc
      361 gtttcctcgc cgacgccacg cccgcacctg ccgcatcgct ccgttgccct cccccgccag
      421 ccgcgccacc tcgtcggaca cccggcggta ctcgcccaac ttgtccagcg cgggccgtac
      481 atgcgcggcg aactcgacca gaaggtcacg cgtcggcact gcccgaagcc caccgggaag
      541 gtcgatcgcc cgcccgtcca gaccgtcacg cgcggctttc cagtacgcgg cccgcaacgt
      601 ttcggcggcc acgcgtggcg gtgctccgcc gcgctcgaac tcatcgagcg cggtcatgac
      661 cgccgcccga atcaacgtcg ctaacagcac cgtctcggca acggtcgacg gcacgtcggc
      721 cacccgcact tcgatggtcg ggaatctcga cgacggccgc acatcccaat acaccatgcc
      781 gtcgtcgagc acggcgcccg tgtccatcag catccgcacg gtcgcgtagt agtcctcgac
      841 cgattcgaag aacggaggcg ggcctgcgct gggccaccgc gcccacagga tgctgcgcca
      901 actggcgtaa ccggtctcgg cgttgcgata gatcgcggag ttggcggtca acgccaaaag
      961 cagtggtagc catggccgca accggttgct gacctggacc gccgcctccc ggtttggtac
     1021 cgcgacgtgc acatggcagc cgcagatgcc ttgctcgtgg gcgatcatgc cgaagttctc
     1081 gccgatcttg cggtaccgcg gcgtgtcggt gatcgggaag tggtgcggca ccgagggcgg
     1141 gagcgccacc gcgagcaacc gcgcgccgca cgctgatgcc gcatccgctg cggcacggcg
     1201 cagccttgtc agctccacgc ggagttcgtc actggtggcg acgacgcggg aggtcgtctc
     1261 gacctggcag ctggtgagtt cgagctgaag ttgcacgcca cgtgcctcag cctgcgcagc
     1321 gaccgcttga ttggcggcga ccggttcgcc ggtgtcatta tcgacgagga ggaactcctc
     1381 ttcgactcca atcgtcggat gctcgtccat ggtgcatgcc attcttccac cgatgctggg
     1441 aagtcactga agccgaggcg atttcaccgc attatgcgcc ggaatgtggc gctgacgtgt
     1501 catagtgact tcacgcgaca tcggatgcgg gagggtcggg gtggccaaat ccgttgggtc
     1561 agcaaacatt atctgttgcg cgttgacgtt gctggtcgga acagttggtt gttcgaaccc
     1621 cccgaaaact gtcaccaaag aagacgtcgc caaccagatc aagcagaaga aatgggacga
     1681 ggcgggcaac aagccggaat ccgtgtcgtg tccgatcgat ctcaggccgg tcgtgggggc
     1741 gaaagtggtg tgcgagatga cgatcaacgg ccaagcggtg ttcgtcgacg tcaccgtctc
     1801 gggcattgat ggcgacaacg tacaactcga catgcggggg catcgttgac gccagcgaaa
     1861 caacaggcga ttctgacctg atgggtgcag aacagcagtc acgaaagctc ggcgatccgg
     1921 tggcgcggct gctgcggctg ccggtgtcga ccaaagagtt catcgagaag atcgtcacgg
     1981 gaagcgtcaa cgaatccggc cggcgaacgc tacacgtgct cttcaccacg tgggacctgg
     2041 cgggcggtgg accgttcgcg gccagtgccg tcgcgtccac cggcatggcg aagaccgtcg
     2101 acatcgtcga gaagagcctg acggttcccg ttttcagccc gctgctgaag aagtt
//
LOCUS       DASWBF010000203        11161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1267497,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000203 DASWBF010000000
VERSION     DASWBF010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11161
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267497"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..346)
                     /gene="rsrA"
                     /locus_tag="VF874_07225"
     CDS             complement(50..346)
                     /gene="rsrA"
                     /locus_tag="VF874_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEX9175885.1"
                     /translation="MSDSEFRSPVGPVDPEHPDCAAVMAEVWTLLDGECTPETRDKLR
                     HHLEACPGCLRHYGVEERIKQLIAAKCGGEKAPERLRQRLLLEISRTTIIRYQA"
     gene            complement(343..1002)
                     /locus_tag="VF874_07230"
     CDS             complement(343..1002)
                     /locus_tag="VF874_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9175886.1"
                     /translation="MTDVDGAGDASSDGVAPSEQPRQETDAELTARFERDAIPLIDQL
                     YGGALRMTRNPADAEDLLQETMVKAYAGFRSFKEGTNLKAWLYRILTNTYINSYRKRQ
                     RQPAEYPTEEITDWQLAANAEHSSTGLRSAEVEALEALPDSEIKEALQALPEEFRMAV
                     YYADVEGFPYKEIAEIMDTPIGTVMSRLHRGRRQLRSLLADVARERGFLRGHTTEEVS
                     S"
     gene            1234..2079
                     /locus_tag="VF874_07235"
     CDS             1234..2079
                     /locus_tag="VF874_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9175887.1"
                     /translation="MSLSGKTMFVSGASRGIGLAIAKRAAADGANIALIAKTAEPHPK
                     LPGTIYTAAKEIEEAGGQALPIVGDIRDGDVVVGAVAKAVQQFGGIDICVNNASAINL
                     GSITEVPLKRFDLMNGIQVRGTYAVSQACLPHMRGRENPHILTLSPPVRLEKKWLTPT
                     AYMMAKYGMTLCALGMAEELRDEGIASNTLWPRTTVATAAVLNLLGGEEAMSRSRKPE
                     VYADAAYVVVNKPAREYTGNTLLCEDVLVENGVSDLSVYDCVPGSTLGVDLWVDTPNP
                     PGYSG"
     gene            complement(2111..2884)
                     /locus_tag="VF874_07240"
     CDS             complement(2111..2884)
                     /locus_tag="VF874_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX9175888.1"
                     /translation="MCGRFAVTTDPKLLAEKIHAIDEATSAQKGSAGPNYNVAPTTTI
                     ASVVSRHTEPDDDAARRLRLMRWGLVPPWAKAGPDGAPDGKGPLLINARAEKVTTSPA
                     FRSSAKSKRCLVPMDGWYEWRANPETVAGKKARKTPFYMHAGDGELLLMAGLWSTWRP
                     KDAAKDVAPLLSCTIITTDAAGPLAEIHDRMPLTISESDWDRWLDPDAPIDEGLLRGH
                     GDLDRIEIREVSTLVNNVRNNGPELIEPAEPQGRQATLL"
     gene            2928..4217
                     /gene="aroA"
                     /locus_tag="VF874_07245"
     CDS             2928..4217
                     /gene="aroA"
                     /locus_tag="VF874_07245"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX9175889.1"
                     /translation="MSTCGRWPAPTAPAPVHATVAVPGSKSQTNRALVLAALGDGTST
                     VTGALRSRDTDLMIGALGTLGLDVEDFDTDVTVSGQIDPGPHATVDCGLAGTVLRFVP
                     PLAALGSSAVTFDGDEQARARPIAPLLDALRTLGVDVDGTALPFVVRGSGSVTGGLVE
                     IDASASSQFVSGLLLSGAAFDDGLTVVHTGASLPSSPHIAMTVAMLRDAGVDVDDSQP
                     RRWRVAPGAIAARQWRIEPDLSNAVPFLAAAAVTGGAVRIESWPLASTQPAGHILDLL
                     RKLDCTVLQTDSHLDVQGPTSYGGIDAELRDVGELTPAVAALAALATPGSVSRLTGVA
                     HLRGHETDRLAALAAEINNLGGNCEETADGLVITAAPLHGGTWHSYADHRMAMAGAIV
                     GLRVPEVAVDDVDTTAKTLPAFPQMWADMLAGQDPTR"
     gene            4214..5194
                     /gene="rsgA"
                     /locus_tag="VF874_07250"
     CDS             4214..5194
                     /gene="rsgA"
                     /locus_tag="VF874_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX9175890.1"
                     /translation="MRPGDYDESDVRVRSGRGSRPRTKTRPEHADARAAMVVTVDRGR
                     WGCVLDNDPSQQVTAMRARELGRTPIVVGDDVDLVGDLSGRPDTLARIVRRGPRRTVL
                     RRTADDTDPTERVVVANADQLLIVVALADPPPRTGLVDRTLIAAYAGGLAPILCLTKT
                     DLASPEPFASQFADLDLTVTTAGRDDPLDVVAPLLAGNVTVLLGHSGVGKSTLVNRLV
                     PQADRAIGEVTGVGKGRHTSTQSVALPLPESGWVIDTPGIRSFGLAHIEPDDVLLAFS
                     DLAEAIKDCPRGCGHLGPPADPECALDALEGPATRRVTAARRLLRVLTET"
     gene            5352..6110
                     /locus_tag="VF874_07255"
     CDS             5352..6110
                     /locus_tag="VF874_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX9175891.1"
                     /translation="MKTRAGRIVGLTAAATAIALSMGVTVTAHGDPEPSPTPTTTTTT
                     TTTLQPPDPQGPGCDAYKKQVPTGNGSFAGMATESLTAAVASNPMLTTLNAAISGQLN
                     PAVNLVGTLNAGTYILFAPVDDAFAQLPPGQLDALKADPTALSNLLSYHVVLGQLYPE
                     DVHGKLTTLQGNQVTVSGKGGDIKVDDTAKVVCGGITTANGVIYMIDTVLNPANSIAS
                     TTTSTTTSPTSSTTTTSTETSAAETPTTTQAPPR"
     gene            complement(6191..7453)
                     /locus_tag="VF874_07260"
     CDS             complement(6191..7453)
                     /locus_tag="VF874_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX9175892.1"
                     /translation="MAISDVPSFAHLTDADIESLAVELDAIRQDIEDSRGERDARYIR
                     RTIAAQRTLEAAGRVILAASSKRSAWWAGAATLGLAKIVENMEIGHNVMHGQWNWMND
                     PEIHSSTWEWDMGGPAKHWRFTHNFAHHKYTNILGMDDDVGYGMLRVTRDQRWKKFNI
                     LNVLWNTILALGFEWGVALQHLEIGKLFKGRANREATQVRIREMSTKAGKQIFKDYVA
                     FPALTSVSPAATFKSTATANAIANVIRNVWANAVIFCGHFPDGAEKFTKSDMEGETKG
                     EWYLRQMLGSANIDAGPVMGFMTGNLSYQIEHHLYPDLPSNRLAEISVKVRQICDKYD
                     LPYTTGSFLVQYGKAWRTIAKLSLPNEYLSATADDAPETRSERMFAELEPGFVGTDPA
                     TGRRRGLKTAIATVRGWRQSRREARQAA"
     gene            complement(7536..8705)
                     /locus_tag="VF874_07265"
     CDS             complement(7536..8705)
                     /locus_tag="VF874_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX9175893.1"
                     /translation="MGSKIHKPTGVVADTLPAIVAGTAERRPVWKTLRALAGRITTPL
                     LPDDYLQLANPLWSARELRGRILEVRRETTDSATLVIKPGWGFEFDYQPGQYVGIGLL
                     VDGRWRWRSYSLTSSPGAAGPATSGRSTSRSTITITVKAMPEGFLSTHLVGGVAPGTI
                     VRLAAPQGNFVMPDPAPASVLFLTGGSGITPVMSMLRTLARRDQITDVLHIHCAPTQS
                     DVMFADELAELARDHPGYRVRVRTTRTQGRLDLRRLADDVPDWRERETWACGPQGMLE
                     AAERVWADAGIAGRLHLERFAVSKAAPRGQGGTVTFARSGKTAAADAATSLMDAGENA
                     GVRMPFGCRMGICQSCVVGLVDGHVRDLRTGVEHEPGTRVQTCISAASGDCVLDV"
     gene            complement(8778..9275)
                     /locus_tag="VF874_07270"
     CDS             complement(8778..9275)
                     /locus_tag="VF874_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175894.1"
                     /translation="MTRVYVPATLAMLQRLVADGQLAPVNGTAFAVTPALREAYAAGD
                     DDELAEVALRDAALASLRLLTSEGSELPRRRAVLVADVNHAAPLPLLDDAVVRLTGPV
                     PLDQVVCVHVDNAAAEDAVAAAVEAVDAADLGDEDAELAVGDAQDHDLAWYAPQELPF
                     LLELL"
     gene            complement(9272..10075)
                     /gene="ppk2"
                     /locus_tag="VF874_07275"
     CDS             complement(9272..10075)
                     /gene="ppk2"
                     /locus_tag="VF874_07275"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005176929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX9175895.1"
                     /translation="MPVQVYESELFRLQTEFVKLQEWTRHSGTRIVVVFEGRDAAGKG
                     GTIKRLTEFLSPRVVRIAALPAPSDRERGQWYFQRYVAQLPARGEIVLFDRSWYNRAG
                     VEKVMKFCTPQEHSLFLEQAPIFENMLIDDGIELRKYWFSVSDNEQLRRFRSRLTDPV
                     RQWKLSTMDLESVYRWEDYSRARDQMMTVTDTPKSPWYVVESDSKKRARLNMMAHLLS
                     TVDYTDVPQPRVELPDTPVHSGTYERPPRHQYNYVPDHAAYLVGDPELT"
     gene            complement(10171..>11161)
                     /locus_tag="VF874_07280"
     CDS             complement(10171..>11161)
                     /locus_tag="VF874_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEX9175896.1"
                     /translation="NGMSALEIGHVIADRTQRPPEFGEDIWIPAHEPSNFELVMGAVG
                     DWMARPTTQLNAFRSAVDDVVTNAGYLADMGRQLTHTARTVLRGTAPDSPLNTQVSRN
                     RRFTVATGRLEDYRKLRARYECDVNDVVLAVVAGALRNWLMSRGEPVTTTSSVRAMAP
                     MSVYPEADLETNAGPGQAISEVSPFLVDLPVGEGNPVVRLSQIAHATESHPTASSLVD
                     ARTIVTLSGFPPPTLHAMGIRVATNYSARVFNLLMTNVPGPQAQMYIAGAKLLETYAV
                     PPLLRNQVLAIGVTSYCGMLYFGINADRDAMSDVEMLPTLLQESLDELIEAAQ"
BASE COUNT         1770 a         3821 c         3718 g         1852 t
ORIGIN      
        1 aaaaccagaa ccaagaccct gtttatcgga aactcaggct aggcgcggac taggcctggt
       61 atctgatgat cgtggtgcgg ctgatctcca gcagcagccg ctgacgtagc cgctcgggcg
      121 ctttctcacc gccgcactta gccgcgatga gctgcttgat gcgctcctcg acgccgtaat
      181 ggcgcaggca gccggggcag gcctcaaggt ggtggcgcag cttgtcgcgg gtctcgggcg
      241 tgcactcccc gtcgaggagt gtccacacct cggccatcac ggccgcgcag tcgggatgtt
      301 cggggtcgac agggccaaca ggcgaacgaa actcgctgtc gctcacgatg acacctcttc
      361 cgtcgtgtgc ccgcgcagga atccgcgttc cctcgccaca tcggcgagca ggctacgcag
      421 ctgacgcctg ccgcgatgca gccgcgacat caccgtcccg atcggcgtgt ccatgatctc
      481 ggcgatctcc ttgtacggga agccttcgac gtcggcgtag tacacggcca tccggaattc
      541 ctccggcagc gcctgcaatg cctctttgat ctcactgtca ggcagcgcct cgagcgcctc
      601 gacctccgct gagcgcagcc ccgtggacga atgctccgcg ttggccgcca gctgccagtc
      661 ggtgatctcc tcggtcggat actccgccgg ctgccgctgc cgcttgcggt agctgttgat
      721 gtaggtgttg gtcaggattc ggtacagcca cgccttgagg ttggtgccct ctttgaacga
      781 ccggaagccg gcgtaggcct tgaccatcgt ctcttgcagc aggtcttcgg cgtcggcggg
      841 attgcgcgtc atccgcagcg caccgccgta gagctggtcg atcagcggga tcgcgtcgcg
      901 ttcgaagcgt gcggtcagct cggcgtcggt ctcttgccgg ggctgctccg acggcgcgac
      961 accgtccgac gatgcgtcac ccgcaccatc gacgtcggtc attacggtcc ctctccacgc
     1021 gtcgtcgaac gcccacaccg gtcgttcgag gcacgccgtc gtcacccaga ctccttgctc
     1081 aatcgtagaa ccgccgagcc gctaggccct gactgcaccc aacaacgcca gttcagttcg
     1141 tcgacattcc ccgccgaggc ggtaactggc gcgggaaact gcgagtgcgc accacgtgga
     1201 ataccgtttc ggcacggggc tagggtcgtc gccatgtccc tttccggcaa gaccatgttc
     1261 gtctcgggtg ccagccgtgg tatcggcctc gcgatcgcca agcgcgcggc ggcagacggc
     1321 gccaacatcg cgctgatcgc caagaccgcg gaaccccacc ccaagctgcc cggcaccatc
     1381 tacaccgccg ccaaggagat cgaagaggcc ggcggtcaag cgctgccgat cgtcggcgac
     1441 atccgcgacg gcgacgtggt ggtcggcgca gtggccaagg cggtgcagca gttcggcggc
     1501 atcgacatct gcgtcaacaa tgcctcggcg atcaacctgg gctcgatcac cgaggtgccg
     1561 ctcaagcgct tcgacctgat gaacggcatc caggtgcggg ggacctatgc ggtgtcgcag
     1621 gcatgccttc cgcacatgag gggccgggag aacccacaca ttctgacgct gtcccccccg
     1681 gtcaggctgg aaaagaagtg gctgacacca accgcgtaca tgatggccaa gtacgggatg
     1741 acgctgtgcg cgctcgggat ggccgaggaa ctgcgcgacg agggcatcgc gtccaacacg
     1801 ttgtggccgc ggacgacggt ggccaccgcg gcggtgctga acctgctcgg cggcgaggag
     1861 gcgatgtccc ggtcgcgcaa gccggaggtg tacgcggacg ccgcgtacgt cgtcgtcaac
     1921 aagcccgccc gcgaatacac cggcaacacg ctgctgtgtg aggacgtgct ggtggaaaac
     1981 ggcgtcagcg acctgtcggt gtacgactgc gtccccggca gcaccttggg cgtcgacctg
     2041 tgggtggaca cccccaaccc gccggggtac agcggctgat gttcttcgcg caagcgctca
     2101 tcaccgccac ctagagcagc gtcgcctggc gcccctgcgg ctcggccggc tcgatcagct
     2161 ccgggccgtt gttgcgcacg ttgttgacca gggtcgagac ttcgcgaatt tcaatccggt
     2221 cgaggtcgcc atgcccgcgc aggagtccct cgtctatcgg ggcgtccggg tcgagccagc
     2281 ggtcccagtc gctttcactg attgtcagcg gcatccggtc gtggatctcg gcgagcggac
     2341 ccgcggcatc ggtggtgatg atcgtgcaac tcaacagcgg cgcaacatct ttcgccgcgt
     2401 ctttcggacg ccacgtcgac cacagccccg ccatcaacag cagctcgccg tcaccggcat
     2461 gcatgtagaa cggggtcttg cgcgccttct tgcccgccac cgtctccgga ttggcccgcc
     2521 actcatacca gccgtccatc ggaacgaggc agcgcttgct cttcgccgac gaacggaacg
     2581 ccggcgacgt ggtcaccttc tcggcgcgcg cgttgatcag caggggaccc ttgccgtccg
     2641 gcgccccgtc gggtccggcc ttggcccacg gcggcaccag tccccaccgc atcaaccgca
     2701 gccggcgcgc cgcgtcgtcg tcgggttcgg tgtgccggct caccaccgat gcgatcgtcg
     2761 tcgtcggcgc gacgttgtag ttgggacccg cgctgccctt ctgcgccgat gtggcctcgt
     2821 cgatcgcgtg gatcttctcg gccaacagct tcggatcggt ggtcaccgcg aatcgcccgc
     2881 acatggctcc atggtggcat cgtcgcccga cacgcgatga tggagccgtg agtacctgcg
     2941 gacgctggcc ggccccgaca gcaccggccc ccgtgcacgc cacggtcgcc gtcccaggct
     3001 cgaagtcaca gaccaaccgc gctttggtgc tcgcggcgct cggtgatgga acgtcgacgg
     3061 tcaccggcgc gctgcgcagc cgcgacaccg acctgatgat cggtgctctg ggcacgctcg
     3121 gattggacgt cgaggacttc gacacggacg tcactgtgtc gggccagatc gacccgggcc
     3181 cccatgcgac cgtcgactgc gggctggccg gaaccgtgct gcgtttcgtg ccgccgctgg
     3241 ccgcgctggg cagctcagcc gtcaccttcg acggcgacga gcaggcccgc gcccggccga
     3301 tcgcgccgct gctcgacgcg ttgcggacgc tcggcgtgga cgtagacggc accgcgttgc
     3361 cgttcgtcgt gcgcggcagc ggctcagtga cagggggcct cgtcgagatc gacgcatcgg
     3421 cgtcgtcgca gttcgtgtcg ggactgctgc tgtcgggcgc ggcgttcgac gacggcctga
     3481 cggtggtgca caccggcgcc agccttccgt cgtcgccgca cattgcgatg accgtcgcga
     3541 tgctgcgcga cgccggcgtg gacgtcgacg attcccagcc gcgccgctgg cgggtcgcgc
     3601 cgggcgcgat cgccgcccgg cagtggcgca tcgagcccga cctctccaac gcggtgccct
     3661 ttctcgccgc ggccgcggtg acgggcggcg ccgtgcgcat cgagagctgg ccgctggcca
     3721 gcacccaacc ggccggccac attctcgacc tgctgcgcaa gctcgattgc actgtgctgc
     3781 aaacggattc gcatctcgac gtccagggtc caaccagcta cgggggcatc gatgccgagc
     3841 tgcgcgacgt cggcgagttg acccccgcgg tggcggcact ggccgcgttg gcgacaccgg
     3901 gctcggtctc ccggttgacc ggcgtcgccc acctgcgcgg ccacgagacc gaccggctgg
     3961 ccgcgctcgc ggcagagatc aacaacctcg gcggcaactg tgaggagacc gccgacgggc
     4021 tggtgatcac ggccgcgccc ctgcacggcg gcacctggca ctcgtacgcc gaccaccgga
     4081 tggcgatggc gggcgcgatc gtcggccttc gggtgcccga ggtggccgtc gacgatgtcg
     4141 acaccaccgc caagacgctg ccggccttcc cacagatgtg ggccgacatg ctggccggcc
     4201 aggatcccac gcgttgaggc ctggggacta cgacgagtcc gacgtccggg tgcgctcggg
     4261 ccggggctct cgcccgcgaa ccaagacccg ccccgagcac gccgacgcgc gcgcggcgat
     4321 ggtggtgacc gtcgaccggg gccgctgggg gtgcgtgttg gacaacgacc cgtcacagca
     4381 ggtcaccgcg atgcgggcgc gcgaactggg ccgcaccccg atcgtggtgg gcgacgacgt
     4441 cgacctggtc ggtgacctgt cggggcgccc ggacacgctg gcgcggatcg tgcgccgcgg
     4501 acctcgacgg accgtgctgc ggcgcactgc cgacgacacc gacccgaccg agcgggtggt
     4561 ggtcgccaac gccgaccagc tgctgatcgt ggtcgcactg gccgacccac cgccgcgcac
     4621 cgggctggtg gaccgaacgc tgatcgccgc ctacgccggc gggttggcgc cgatcctgtg
     4681 cctgaccaag accgatttgg cgtcgccgga gccgttcgcg tcgcagttcg ccgacttgga
     4741 cctgaccgtg acgacggcgg gtcgcgacga tccactggac gtcgtcgcgc cgctgttggc
     4801 ggggaatgtg acggtgttgc tgggccactc cggggtgggc aagtccacgt tggtgaatcg
     4861 ccttgtgccg caagcggatc gggccatcgg cgaggtgacg ggtgtgggca aggggcggca
     4921 cacgtcgacg cagtcagtgg cgttgccgct gccggagtca gggtgggtga tcgacacgcc
     4981 gggtatccgg tcgttcgggc tggcccacat cgaacccgac gacgtgctgc tggcgttctc
     5041 cgacctggcg gaggcgatca aggactgccc ccgcgggtgc gggcacctcg gtccgcccgc
     5101 cgatccggaa tgcgcgctgg acgcgttgga gggtccggcg acgcgacggg tgacggcggc
     5161 ccgccggctg ctgcgggtgc tgacggaaac ctagcctcac ctgcatcgtt ttgggggagg
     5221 tcgtccccgc ccttacgatg cgatgcagtg ttgaacgcgg cgtcgcggca gcggtgatca
     5281 ggctgccagc cgcaggcgac gggcaggcat tggaccgccc agggcgctga atgtagacga
     5341 gaggaaccga agtgaagact cgtgccggca ggatagttgg ccttactgcg gccgcgaccg
     5401 cgatcgcgtt gtcgatgggc gtgacggtga cggcacatgg cgatcccgaa ccgtcaccga
     5461 cgccgacaac gacgacgacg acgacgacga cgttgcagcc acccgaccct cagggtcccg
     5521 ggtgcgacgc gtacaagaag caggtcccga ccggtaacgg ttcgttcgcc gggatggcga
     5581 ccgaatcgct gactgccgcg gtggccagca acccgatgct gacgacgctg aacgcggcta
     5641 tctcgggaca gctcaacccg gcggtcaacc tggtcggcac gctgaacgcc ggtacgtaca
     5701 ttctgttcgc cccggtcgac gatgcgttcg cgcagctgcc gcccggacag ctcgacgcgc
     5761 tgaaggccga tcccacggcg ctgagcaacc tcctcagcta ccacgtcgtt ctgggtcagc
     5821 tctatccaga ggacgtgcac ggcaagctga ccacgctgca gggcaaccaa gtcaccgtca
     5881 gcggaaaggg cggcgacatc aaggtcgacg acaccgccaa ggtggtctgc ggcggaatca
     5941 cgaccgctaa cggcgtgatc tacatgatcg acaccgtgtt gaatcccgcg aactcaatcg
     6001 catcgaccac gacgtcgacc acgacgtcgc cgacctcgtc aaccaccacc acctcgaccg
     6061 agacgagtgc ggccgagaca ccgacgacga cgcaggcccc gcctcgctag gcgtgttagt
     6121 gccgaggcgg taaccaccgc ggaaaagcac gagtacaccc cgcgtggaat accgtttcgg
     6181 cgcacggggg ttaggcggct tggcgggctt cccgtcggct ctggcgccaa ccgcgcacgg
     6241 tcgcgatcgc ggtcttgagg ccgcggcgcc gtcccgtcgc agggtcggta ccgacgaatc
     6301 cgggttccag ctcggcgaac atcctctcgc tgcgcgtctc cggtgcgtcg tcggcggtcg
     6361 cgctcaggta ttcgttcggc agcgacagtt tggcgatcgt gcgccacgcc ttgccgtact
     6421 gcacaaggaa tgagccggtg gtgtagggca ggtcgtactt gtcgcagatc tggcgcacct
     6481 ttaccgagat ctcggccagc cggttgctcg gcagatcggg gtacaggtga tgctcgatct
     6541 gatacgacag gttgcccgtc atgaacccca ttaccgggcc cgcgtcgatg ttcgcgctgc
     6601 caagcatctg gcgcaggtac cactcgccct tggtctcgcc ctccatgtcg ctcttggtga
     6661 atttctctgc gccgtcaggg aaatggccgc agaagatcac cgcgttggcc cacacgttgc
     6721 ggatgacgtt ggcaatcgcg ttggcggtgg cggtggactt gaacgtcgcg gccggcgaaa
     6781 ccgacgtcag cgccgggaac gcgacgtagt ccttgaatat ctgcttaccg gccttggtcg
     6841 acatctcacg gatccgcacc tgcgtcgcct cgcggttggc ccgtcccttg aacagcttgc
     6901 cgatctccaa gtgctgcaac gcaactcccc actcgaaacc gagcgcgagg atcgtgttcc
     6961 acagcacgtt cagaatgttg aatttcttcc agcgctggtc gcgggtgacg cgcagcatgc
     7021 cgtagccgac gtcgtcgtcc atgccgagaa tgttcgtgta cttgtggtgc gcgaagttgt
     7081 gggtgaagcg ccagtgcttg gcggggccgc ccatgtccca ctcccacgtc gaggagtgga
     7141 tctcgggatc gttcatccaa ttccattggc cgtgcatgac gttgtggccg atctccatgt
     7201 tctcgacgat cttcgccagg ccaagcgttg cggcaccggc ccaccacgcc gaacgtttgg
     7261 agctggccgc caggatcacc cggccggccg cctcgagggt gcgctgtgcg gcgatcgtgc
     7321 gacggatgta gcgcgcgtcg cgttcaccgc gggagtcttc gatgtcctgg cgtatcgcgt
     7381 cgagttcgac ggccaggctt tcgatgtccg cgtccgtgag gtgggcgaac gaggggacgt
     7441 ctgagatcgc caacgcattc tccttccgtc tggccgtctg gtcgctacct acgttagcgt
     7501 aacctacgac cgcgtaggtt accagtaagt aatcgttaga cgtcgagcac gcaatcaccc
     7561 gacgccgccg agatgcacgt ctggacgcgc gtgcccggct catgctcgac accggtgcgc
     7621 agatcgcgga cgtggccgtc gaccaggccc accacacacg actggcaaat acccattcgg
     7681 cagccgaaag gcattcgtac accggcgttt tcgccggcat ccatcagcga cgtggccgca
     7741 tcggccgccg ccgtcttgcc gctgcgcgca aacgtcaccg tgccgccctg cccgcgcggc
     7801 gccgccttgc tgaccgcgaa ccgctcgagg tgaagccggc cggcaatgcc tgcgtcggcc
     7861 cagacccgct cggcggcctc cagcattccc tgcggtccgc acgcccacgt ctcgcgctcg
     7921 cgccagtccg gcacgtcgtc ggccagccgg cgcagatcca ggcggccctg cgtacgcgtc
     7981 gtcctcaccc gcacgcggta gccgggatga tcacgcgcca gctcagccaa ctcgtcggcg
     8041 aacatcacat ccgattgtgt tggggcgcaa tggatgtgaa gaacgtcggt gatctggtcc
     8101 cgacgggcca gggtgcgcag catcgacatc accggcgtga tgccggagcc gccggtgagg
     8161 aacagcaccg acgccggcgc cgggtcgggc atcacgaagt tgccctgcgg tgcggccaac
     8221 cgcacgattg tgcccggcgc gacgccaccg accagatgcg tcgacaggaa gccttcgggc
     8281 atcgccttga cggtgatcgt gatcgtcgac cgcgacgtgc tccggccgga tgtcgccggc
     8341 cctgcggctc cgggactgga cgtcaacgag taggaccgcc accgccaccg gccgtcgacg
     8401 agcaggccga tgccgacgta ctggcccggc tgatagtcga actcgaatcc ccaccccggc
     8461 ttgatgacca gggtcgcgga gtcggtggtc tcgcgccgga cctcgaggat ccggccgcgc
     8521 aactcgcgag ccgaccacag cggattggcg agttgcaggt agtcgtcggg cagcagcggg
     8581 gtggtgatcc ggccggccaa cgcgcgcagc gtcttccaca ccgggcgcct ttcggcggtg
     8641 ccggcaacga tcgctggcag cgtgtcggcg acgaccccag tgggcttgtg gattttcgag
     8701 cccatggcta gtaactcccc gcagttcgat ccggctaacc tacggtaccg taacctacgg
     8761 tcaccgaaca actcggatca gagcaattcg agcagaaaag gcagttcctg cggcgcatac
     8821 caggccagat cgtggtcttg cgcatcaccg acggcgagtt cagcatcctc gtcgccgagg
     8881 tctgcggcat caacggcctc caccgccgcc gccaccgcgt cctcggcggc cgcgttgtcg
     8941 acatggacgc agaccacctg atccagcggc accgggccgg tgagccgcac cacagcgtcg
     9001 tcgagcaggg gaagcggcgc cgcgtggttg acgtcggcga cgagcacggc cctgcggcgc
     9061 ggaagttccg acccttccga tgtgagcagc cgcagcgagg ccagcgccgc gtcgcgcagc
     9121 gccacctcgg cgagctcgtc gtcgtcaccg gcggcgtagg cctcgcgtag cgcgggtgtc
     9181 accgcaaacg cggtgccgtt gacgggggcc agctggccgt cggcgaccag ccgctgcagc
     9241 atcgccaggg tcgccggtac gtagacccgc gtcacgtcag ctcggggtcg ccgaccagat
     9301 aggccgcgtg gtcggggacg tagttgtact gatggcgcgg cggacgctcg taggtgcccg
     9361 agtggacggg cgtgtccggc agttccaccc tcggctgtgg cacgtcggtg tagtcgacag
     9421 tggacagcag gtgcgccatc atgttgagcc gggcgcgctt cttggagtct gattcgacga
     9481 cgtaccacgg actcttgggc gtgtcggtga cagtcatcat ctggtcgcgg gcccgcgagt
     9541 agtcctccca ccgatacacc gactccaagt ccatcgtcga aagcttccat tgccgcaccg
     9601 ggtccgtgag gcgcgagcgg aaccgccgca actgctcatt gtcagacacc gagaaccagt
     9661 acttgcgcag ctcgatgccg tcgtcgatga gcatgttctc gaagatcggc gcctgttcaa
     9721 gaaacagtga atgctcttgg ggtgtacaga acttcatcac tttctcgacg ccagcacggt
     9781 tgtaccagga ccggtcgaac agcacgatct cgccacgcgc cggcagctgg gcgacatagc
     9841 gctggaagta ccactgcccg cgttcgcggt cggacggcgc cggcaacgcc gcgatgcgga
     9901 cgacgcgggg gctgaggaac tcggtgagcc gcttgatggt gccccctttg ccggcggcgt
     9961 cgcggccttc gaagacgacg acgatgcgcg tgccggagtg ccgggtccat tcctgcagct
    10021 ttacgaattc cgtttgcagg cggaacaatt ccgactcgta cacctggacg ggcatcttga
    10081 gccgcttgcg attcgtcgcc gagccgtccg agccgtccga cccgtcccgc gctttcttct
    10141 tgcccatcgg cgaatcgtat cgccgccgac ctactgcgcg gcctcgatca gttcgtcgag
    10201 ggactcctgg agcagagtgg gcagcatctc gacgtcgctc atcgcgtccc ggtcggcgtt
    10261 gatgccgaag tacagcattc cgcaatacga ggtcaccccg atcgccagca cctggttgcg
    10321 cagcagcggc ggcacggcat acgtctcgag cagcttcgcg ccggcaatgt acatctgcgc
    10381 ctgcggaccg ggcacattgg tcatcaacag gttgaacacc cgcgccgagt agttggtggc
    10441 cacccggatg cccatcgcgt gcagcgtcgg cggcgggaag cccgacaagg tgacgattgt
    10501 cctggcgtcc accaggctcg aggcggtggg atggctttcg gtggcgtgtg cgatctgcga
    10561 cagccgcacc accgggttgc cctccccgac cggcaggtcc accaggaacg gcgacacctc
    10621 gctgatcgcc tgaccgggac ccgcgttggt ctcgaggtcg gcttccgggt acaccgacat
    10681 cggggccatc gcccgcacgc tcgacgtcgt cgtcacgggc tcaccccgcg acatcagcca
    10741 gttgcgcaac gcccccgcca ccacggccag cacgacgtcg ttcacgtcgc actcgtagcg
    10801 ggcccgcagc ttgcggtagt cctccagccg cccggtggcc acggtgaacc ggcggttgcg
    10861 cgacacctgc gtattcaacg ggctgtccgg ggcggtgcca cgcagcacgg tgcgggccgt
    10921 gtgcgtcagc tggcggccca tgtcggcgag gtagccggcg ttggtgacaa cgtcgtcgac
    10981 cgcggaccgg aacgcgttca gttgagtggt gggccgcgcc atccagtccc cgacggcgcc
    11041 catcaccagc tcgaagttcg acggttcgtg cgccgggatc cagatgtcct cgccgaactc
    11101 gggcgggcgc tgcgtccggt cggcgatcac gtgtccgatc tccagcgccg acatcccgtt
    11161 g
//
LOCUS       DASWBF010000204         6765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_604092,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000204 DASWBF010000000
VERSION     DASWBF010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604092"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VF874_07285"
     CDS             <1..675
                     /locus_tag="VF874_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9175897.1"
                     /translation="RKAFGAYLIDQPLMRNVLADLAVEAEAATIVAMRMAGATDNASR
                     GDETEALLRRIGLAAAKYWVCKRATPHAGEAMECLGGNGYVEDSGMPRLYREAPLMGI
                     WEGSGNVSALDTLRAMATRPECVEVFFGELAKTAGQDRRLDKHVDALKPQLEDFDTIQ
                     YRARKVAEDICLALQGSLLVRHGHPAVAEAFLATRLGGQWGGAFGTMPGGLDLAPILE
                     RALVKG"
     gene            672..1610
                     /locus_tag="VF874_07290"
     CDS             672..1610
                     /locus_tag="VF874_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9175898.1"
                     /translation="MTHSIRPVDFDNLKTMTYEVTDRVARITFNRPEKGNAIVADTPL
                     ELSALVERADVDPDVHVILVSGRGEGFCAGFDLSAYAEGSSSAGGGSAYKGTVLSGKT
                     QAINHLPDRPWDPMIDYQMMSRFVRGFSSLMHADKPTVVKIHGYCVAGGTDIALHADQ
                     VIAAADAKIGYPPTRVWGVPAAGMWAHRLGDQRAKRLLLTGDCITGAQAAEWGLAVEA
                     PDPAELDERTERLVQRIAAVPVNQLIMVKLAMNSALYNQGVTNSAMISTIFDGIARHT
                     PEGHAFVAQAREHGFRDAVRRRDEPFGDYGRRASEV"
     gene            1612..2346
                     /locus_tag="VF874_07295"
     CDS             1612..2346
                     /locus_tag="VF874_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX9175899.1"
                     /translation="MPRTLARMTARSVVLSVLLGAHPAWATTAELLRLTADFGLKEST
                     VRVALTRLVSTGDLVRSADGYRLSDRLLARQRRQDDAMRPHVKPWDGTWTTLVITSVG
                     VDPRTRAALRNTLQHNRFGELREGVWLRPDNLEVTLSNDVTHRVRVLHARDDHPAGLA
                     RQLWDLTAWARTAERLLEDMATASDTPGRFVAAAGMVRHLISDPVLPDELLPPDWPAK
                     ALRDSYFKFADELAARRYPTEQLVEA"
     gene            2349..3113
                     /locus_tag="VF874_07300"
     CDS             2349..3113
                     /locus_tag="VF874_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9175900.1"
                     /translation="MSDEVRVERQGAVTTVIINRPEARNAVNGPTAAQLVAAFEEFDA
                     DDTASIAVLWGDNGTFCAGADLKAFGTPESNAVHRTGPGPMGPTRMVLSKPVIGAVSG
                     YAVAGGLELALWCDLRVAEEDAVFGVFCRRWGVPLIDGGTIRLPRLIGHSRAMDIILT
                     GRPVDAAEAYNIGLANRVVPKGQARQAAEELAAELAALPQQCMRSDRLSTLTQWGMSE
                     KDAIDFEFGSLSRVAAEALEGAGRFAAGAGRHGAPA"
     gene            complement(3198..3647)
                     /locus_tag="VF874_07305"
     CDS             complement(3198..3647)
                     /locus_tag="VF874_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HEX9175901.1"
                     /translation="MHRFVAIVIASVAISALAGCGSDSQKNGPAKTTSSTTVDRSTAT
                     ASPTSTTNTSGTQFEVGDTINYGSMDTTETVDCANGKSLNVGGSNNTLTVTGVCKDVT
                     VLGSGNTITLNQVDSTINVVGIDNKLTYKAGDPQIDKFGDSNEVTKG"
     gene            complement(3670..4032)
                     /locus_tag="VF874_07310"
     CDS             complement(3670..4032)
                     /locus_tag="VF874_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HEX9175902.1"
                     /translation="MRTAACCAAAACLIFSALGTPTANARGEGDTHVAGIGQEMTIDC
                     NNATLFVNGSWNVINATGVCYAVTMQGSGNVVIADTITDDITVYGSDQTVMFHNGDPF
                     ILDRGRELGMTNRIDRVA"
     gene            4252..4737
                     /locus_tag="VF874_07315"
     CDS             4252..4737
                     /locus_tag="VF874_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEX9175903.1"
                     /translation="MTAVGCSRTVTGSAQRAIDSGDASAHGYGYTDDRCGLLQDSTVQ
                     EIVGAEQLVRPYIGAVCQYVLMRQSTVIDVTFSWFETGNLERERGLAESNGAQITDVV
                     VERHQAFLARRSVTGNACSATAATNPGVASWWVQVRGDAASDPCQAAQALLAKTLSSD
                     M"
     gene            4752..5246
                     /locus_tag="VF874_07320"
     CDS             4752..5246
                     /locus_tag="VF874_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEX9175904.1"
                     /translation="MALVLLAAAVAGCQAPQPNPALPTVEADGFRSGDCNLVSDDDVN
                     AAAGTGMFTKVVVGDAGCFWQENSMIGSFGAGMGISTWWYRGSDLDVERTLERKAGRE
                     ITELTLDGNSGFQASDANTCSIYVAKGGDVITWSMQTMNTATLPGLCTVIRRLAQLSQ
                     DRVN"
     gene            5281..5865
                     /locus_tag="VF874_07325"
     CDS             5281..5865
                     /locus_tag="VF874_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HEX9175905.1"
                     /translation="MAAARSSGRTTKLSRDVIVNSALTFLDREGWDALTINALAAQLG
                     TKGPSLYNHVNSLDDLRRSVRKRVVGDIIEMLGTVGQGRTRDDAVLVMAGAYRSYAHH
                     HPGRYSAFTRMPLDGGDDPEFTAVARAAAEPVIAVLASYGLDGEDAFYAALEFWAALH
                     GFVLLEMTGVMAGIDVDAVFSDMVLRLATGMQHR"
     gene            6147..6521
                     /gene="rpsL"
                     /locus_tag="VF874_07330"
     CDS             6147..6521
                     /gene="rpsL"
                     /locus_tag="VF874_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX9175906.1"
                     /translation="MPTIQQLVRKGRQDKIAKVKTAALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVKLTSQVEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYKIIRGSL
                     DTQGVKNRKQARSRYGAKKEKS"
     gene            6521..>6765
                     /locus_tag="VF874_07335"
     CDS             6521..>6765
                     /locus_tag="VF874_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX9175907.1"
                     /translation="MPRKGPAPKRPLVNDPVYGSQLVTQLVNKILLKGKKSLAERIVY
                     GALEQARDKTGTDPVVTLKRALDNVKPALEVRSRRVGG"
BASE COUNT         1172 a         2165 c         2329 g         1099 t
ORIGIN      
        1 cgaaaggcgt tcggcgccta cctgattgac cagccgctga tgcgcaacgt actcgccgac
       61 ctcgcggtcg aggccgaggc ggcgacgatc gtcgcgatgc ggatggccgg tgccaccgac
      121 aacgcgtcac gcggcgacga aacggaggca ctactacgcc gcatcggtct ggccgcggcc
      181 aagtactggg tctgcaagcg cgcgaccccg cacgcgggcg aggcgatgga gtgcctgggc
      241 ggcaatggct acgtcgagga ctcgggcatg ccgcggctgt accgtgaggc gccgctgatg
      301 ggcatttggg agggctccgg aaatgtcagt gcactggaca cgttgcgcgc catggcaact
      361 cggcccgaat gcgtcgaggt gtttttcggc gaactcgcca agactgcggg gcaggaccgg
      421 cgcctcgaca agcatgtcga cgcgctcaag ccccaactcg aagacttcga caccatccag
      481 taccgggccc gcaaggtcgc cgaggacatt tgccttgcgc tgcaagggtc gctgctggtg
      541 cggcacggtc atccggcagt ggccgaagca ttcctcgcca cccgcctggg cgggcagtgg
      601 ggcggcgcgt tcggcaccat gcccggcggc ctggacctcg cgccgatcct cgagcgggcg
      661 ctggtcaagg gatgacccac tcgatcagac cggtggactt cgacaacctg aagacgatga
      721 cctacgaggt cacagaccgg gttgcccgaa tcacgttcaa ccggcccgaa aaaggcaacg
      781 cgatcgtcgc cgacacgccg ttggagttgt cggctctggt cgaacgcgcc gacgtcgatc
      841 ccgacgtgca cgtgatcctc gtgtccggac gcggcgaagg cttctgcgcc ggtttcgatc
      901 tcagcgcgta cgcggagggg tcgtcgtcgg ccggcggggg cagcgcctac aaaggcacgg
      961 tgctttccgg aaaaacgcag gcgatcaacc atctgcccga tcggccgtgg gatccgatga
     1021 tcgattacca gatgatgagc cggttcgtcc gcggcttctc cagcctgatg cacgccgaca
     1081 agccgaccgt ggtgaagatc cacggctact gcgtggccgg aggcaccgac atcgcgctgc
     1141 acgccgacca ggtgatcgct gcggcggacg ccaagatcgg ctatccgccg actcgggtgt
     1201 ggggcgtgcc tgcggcgggc atgtgggcgc accgcctcgg tgaccagcgc gcgaaacgcc
     1261 ttctgctgac cggtgattgc atcaccggcg cgcaggcagc cgagtggggg ctggccgtcg
     1321 aagcccccga ccccgcagag cttgacgagc gcaccgagcg gctggtgcag cggatcgccg
     1381 cggtgccggt caaccagctg atcatggtga agctcgcgat gaacagcgca ctctacaacc
     1441 agggcgtgac caacagcgcg atgatctcca cgatcttcga cggcatcgcg cggcacacac
     1501 ccgaaggtca cgcgttcgtc gcgcaagccc gcgagcacgg cttccgcgat gcggtgcgtc
     1561 gccgcgacga gccgttcggc gactacgggc gacgcgcatc cgaggtctga gatgcccagg
     1621 acgctcgccc ggatgacggc ccgctcggtg gtgctgagcg tgctgctcgg cgcacatccg
     1681 gcatgggcga cgacagcgga attactcaga ctgacagctg atttcgggct taaggagtcg
     1741 accgtgcggg tcgcgttgac ccgactggtc agcacaggtg atctggtgcg ctcggccgac
     1801 ggataccggc tgtccgaccg gctgctggct cggcagcgtc gccaggacga cgcgatgcgg
     1861 ccgcatgtca aaccgtggga cggcacctgg accacgctgg tgatcaccag cgtcggcgtc
     1921 gacccccgaa cccgcgctgc gctgcgaaac acactgcagc acaatcgatt cggtgagctt
     1981 cgagaagggg tgtggctgcg gccggacaac ctcgaggtca cgctgagcaa tgatgtgacg
     2041 cacagggtgc gggtgctgca cgcccgcgac gaccatccgg ccgggctggc aaggcagttg
     2101 tgggacttga cggcgtgggc gcgcaccgcc gagcggctac tcgaggacat ggccaccgcg
     2161 tccgataccc cggggcgctt cgtggccgcc gcggggatgg tgcgccacct gatcagcgac
     2221 ccggtgctgc ccgacgagct actgccacct gattggccgg caaaggcgct gcgggacagc
     2281 tatttcaagt ttgccgacga gctcgcggcg cgacggtatc cgaccgaaca actagtggag
     2341 gcatgatcat gagcgacgaa gtacgggtcg agcggcaggg tgccgtgacc acggtgatca
     2401 tcaaccggcc ggaagcgcgc aacgcggtca acggaccgac ggcagcgcaa ctggtcgcag
     2461 cgttcgagga attcgacgcc gacgacaccg cgtcgatcgc ggtgctgtgg ggcgacaacg
     2521 gaacgttctg cgccggtgcc gatctcaagg ccttcggcac accggagtcc aatgccgtgc
     2581 accgcaccgg tcccggcccg atgggcccga cgcgaatggt gttgtccaag ccggtgattg
     2641 gggctgtgag cgggtacgcc gtcgcgggcg gactcgagct ggcgctgtgg tgcgacctgc
     2701 gagtcgctga ggaagacgcg gtgttcgggg tgttctgccg gcgctggggc gtgccgctga
     2761 tcgacggcgg cacgatccgg ctgccgaggc tcatcggcca cagtcgcgcg atggacatca
     2821 tcctgaccgg acggccggtg gacgcggcgg aggcctacaa catcgggctg gccaaccgcg
     2881 tggtgccgaa aggccaagcg cgccaagccg cggaggaact cgcggcggag ctcgccgcac
     2941 tgccgcagca gtgcatgcgc tccgaccggt tgtcgacgct cacccagtgg gggatgtccg
     3001 agaaagacgc gatcgacttc gagttcggca gtttgtcccg cgtggcggcc gaagcactcg
     3061 agggcgcagg gcgattcgct gcgggcgccg gccggcacgg tgcacccgcg taacgctcgt
     3121 cacaacgcga gcgtgcgctt tggtaggcct ctcgacggcg tgttggcgta caaacgcgca
     3181 cgctcgcgaa aaaggggtca gcccttggtg acttcgttgc tgtcgccgaa cttgtcgatc
     3241 tggggatcgc cggccttgta ggtgagcttg ttgtcgatcc cgaccacgtt gatcgtgctg
     3301 tcgacctgat tcagcgtgat cgtgttgcca ctgccgagga ccgtcacatc tttgcagacg
     3361 cccgtgaccg tcagggtgtt gttcgagccg cccacgttca gcgacttgcc gttcgcgcag
     3421 tccaccgttt cggtggtgtc catcgatccg tagttgatcg tgtcaccgac ctcgaactgc
     3481 gttccggacg tgttggtcgt ggaagtcgga ctggccgtgg ctgtcgatct gtcaacggtc
     3541 gtcgagctgg tcgtcttcgc cggaccgttc ttctggctgt cggagccgca gccggccagc
     3601 gcgctgatgg caacggacgc tatgacgatc gcaacgaacc tgtgcatgat tgcccccatt
     3661 tctgaatcgt caggcaacgc ggtcgatccg gtttgtcatc cccagttcgc ggccgcggtc
     3721 caagatgaac ggatctccgt tgtgaaacat cacggtctgg tccgagccgt agacggtgat
     3781 gtcgtcggtg atggtgtcgg cgatcacgac attcccggag ccctgcatgg tcaccgcata
     3841 gcagacgccg gtggcgttga tgacgttcca cgacccgttg acgaacaacg tcgcgttgtt
     3901 gcagtcgatc gtcatctcct ggccgatgcc cgcgacgtgc gtgtcgccct cgccccgtgc
     3961 attcgccgtc ggcgtcccga gtgccgaaaa gatcaggcac gcagcagcgg cacaacatgc
     4021 cgcggttcgc atcgtcgtcg ccccctattc gcatgtcccg acccgcaaag cgtactccgc
     4081 taagcgggtg ggggtacctc ccgttcacgg gggagtgccc ccagtcggcg cgcgggtgcg
     4141 gtagcggttg gcctcttctg cgccaggtcg ccatggcata cacccctcgg tacggtatga
     4201 gcggtgaact ggcgtggccc gacggctgct gtcatggtcg cctcggcgct catgacggcc
     4261 gtcggctgtt cccgcaccgt caccggttca gcccagcgcg ccatcgattc cggcgacgcg
     4321 tcggcgcacg gctacggcta caccgacgac cggtgcggac tgctccagga cagcacggtt
     4381 caggagatcg tcggcgcgga gcagttggtc cggccgtata tcggcgcggt gtgccaatac
     4441 gtgctgatgc ggcagtccac ggtcatcgac gtcacgtttt cctggttcga gaccggcaac
     4501 ctcgagcgtg aacgcgggct ggccgaatcg aacggggcgc agatcaccga tgtcgtagtg
     4561 gagcgtcatc aagccttcct ggcccgccgc tcggtgaccg gcaacgcctg ctcggccacg
     4621 gcggccacca atccgggggt ggcgagctgg tgggtgcagg ttcgtggcga tgcggcgtcg
     4681 gatccctgcc aggcggcgca ggccctgcta gcgaagacgt tgtcgtcgga catgtgatga
     4741 ttcgatcgag gatggcacta gtgctgctcg cggccgcggt cgcgggatgc caagcgccgc
     4801 aaccgaaccc agcgctgccg accgtcgaag ccgacggatt ccgcagcggt gattgcaacc
     4861 tggtctccga cgacgacgtg aacgccgccg ccggaacggg gatgttcacc aaggtcgtcg
     4921 tcggcgacgc gggctgcttc tggcaggaga actccatgat cggctcgttc ggcgccggca
     4981 tgggcatttc gacgtggtgg tatcgcggca gcgacctgga cgtcgagcgg acgctggaaa
     5041 gaaaggcggg ccgagagatc accgagctga cgctggacgg caacagcggc tttcaggcgt
     5101 ccgacgcgaa cacttgcagc atctacgtcg ccaagggcgg cgacgtcatc acctggtcga
     5161 tgcagacgat gaacacggcc acgctgccag gactgtgcac cgtcatccgg aggctcgcgc
     5221 agctgtcgca ggaccgcgtc aactgaccgc tggctgaaca ggacggctaa agtccgcact
     5281 gtggcagcgg cgcgctcgtc gggacgaacg acgaaactca gccgcgacgt catcgtcaac
     5341 tccgcgctga ccttcctcga ccgcgagggc tgggatgcgc tgacgatcaa tgcgctcgcc
     5401 gcccagctgg gcaccaaggg cccgtcgctg tacaaccacg tcaacagcct ggatgacctg
     5461 cgccgatcgg tgcgcaagcg ggtcgtcggc gacatcatcg agatgctcgg caccgtcggt
     5521 cagggtcgca cccgcgacga tgcggtgttg gtgatggccg gggcgtaccg cagctacgcg
     5581 catcaccacc cgggccggta ttcggcgttc acccggatgc cgctggacgg cggcgacgac
     5641 cccgagttca ccgcggttgc gcgtgccgcc gcagagccgg tgatcgcggt cttggcgtcc
     5701 tacgggctcg acggcgagga cgcgttctac gcggcgctgg agttctgggc cgcgctgcac
     5761 ggcttcgtgc tgctggagat gacgggcgtg atggccggca tcgacgtcga cgcggtgttc
     5821 tccgacatgg tgctgcggct ggcgaccggg atgcagcacc ggtaggtcgc gcgtgctcga
     5881 agacgtaggt tgacctgcag ttagttgctg cccaacgagt ttgttccgac tgcggccacc
     5941 ctggtatcgt ggtcggtcgt gcctggtaga taaggcgatg cggtgacgca cgtccgcgcc
     6001 gggccaattc gcatgtcccc gatgtcagac cgcggcggac gcatgcgaca cgcccggcct
     6061 cggggtgacc gagcggggcg aaaccgcagt acagagacat ttgacaacga cgaactgaag
     6121 aacgacgcga cagagaaagc cggtagatgc caaccatcca gcagctggtc cgcaaagggc
     6181 gccaggacaa gatcgccaag gtgaagaccg cggcgctcaa gggcagcccg cagcgccgcg
     6241 gcgtgtgcac ccgcgtgtac acgacgacgc cgaagaagcc gaactcggct cttcgcaagg
     6301 tcgcgcgcgt gaagctgacc agccaggtcg aggtcaccgc ctacatcccc ggtgagggcc
     6361 acaacttgca ggagcactcg atggtgctgg tgcgcggcgg tcgtgtgaag gacctgcccg
     6421 gcgtccgcta caagatcatc cgcgggtcgc tggacaccca aggcgtgaag aaccgcaagc
     6481 aagcccgcag ccgctacggc gccaagaagg agaagagctg atgccgcgca agggacccgc
     6541 gcccaagcgt ccgctggtta acgacccggt ctacggatcg cagctggtca cccaattggt
     6601 gaacaaaatt ctgctgaagg ggaagaaatc gctggccgaa cgcattgttt atggtgcgct
     6661 cgagcaggct cgcgacaaga ccggcaccga cccggtggtc accctcaagc gcgctctcga
     6721 caacgtcaaa cccgccttgg aggttcgtag ccgccgcgtg ggtgg
//
LOCUS       DASWBF010000205         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1068034,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000205 DASWBF010000000
VERSION     DASWBF010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068034"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF874_07340"
     CDS             complement(<1..187)
                     /locus_tag="VF874_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952333.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9175908.1"
                     /translation="MPQPSILSVLRERAGLQPDDTAFTYTDYEQDWAGVEDSLTWSQA
                     YRRVVNVALEVSRHGSVG"
     gene            316..1668
                     /locus_tag="VF874_07345"
     CDS             316..1668
                     /locus_tag="VF874_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9175909.1"
                     /translation="MSDPRPPAHPQRRSQVLSWALWDFGATGVNAIVVTFVFSVYLTS
                     SVGHDLPGETSPASWLGRALALAGLVVALLAPVTGVWVDAPWRRRRVLAVLTGAVVLL
                     TAAMSLIRDDYHYLWLGLVLLACTAACNDLATVPYNAMLRQVSTLDSPGRISGFGLAM
                     GFLGSVVLLLVVYFGFIAGHGDAHGLLQLPAADGQNVRAAVLLAAVWFALFALPVFIA
                     VPPPAPKPEGPREAVGFLGTYRTLWSEITGEWRRDRNIVYYLLASAVFRDGLTGVVTF
                     GAVLGVNVYGISGADVLLFGVGASVIAAVGAVSGGLLDDRLGSKPVIVGSLIAMITVA
                     VTLMALSGAPAFWVCGLLLCLFIGPTQSSARTLMLRMSADGKEGMAFGLYTTTGRAVS
                     FLAPWLFSSFIDLFGTDRAGMGGLCVVLVAGLVGMLMVRVPGRSLAHTSSSASTSKPR
                     "
BASE COUNT          247 a          619 c          658 g          393 t
ORIGIN      
        1 ccccgaccga cccgtgacgg ctgacctcga gcgcgacgtt gaccacccgc cggtatgctt
       61 gcgaccaggt caggctatct tcgacacccg cccaatcctg ctcgtaatcg gtatacgtga
      121 acgcggtgtc atcgggctgc agaccggcac gctcgcgcag cacggacagg atcgacggct
      181 ggggcatgcg cgccacgtta ccgcccggct tgcgcctgtg ttcgcgagat cacgggttcg
      241 catgcaacga aacccgtttg aaccaaccgg aatcggcgct gctgacatgg gccggcctgt
      301 atatggtggg cgaccatgag cgatccgcgg ccgcccgcgc atcctcagcg gcggtcacag
      361 gtcctttcct gggcgctgtg ggacttcggc gcgacgggtg tgaacgcgat cgtggtgacg
      421 ttcgtgttct cggtgtatct gaccagcagc gtcggccacg acctgccggg ggagaccagc
      481 cctgccagct ggctcgggcg cgcgttggct ctcgcgggtc tggtggttgc tttgttggcg
      541 cctgtcaccg gtgtctgggt cgacgcccca tggcggcgcc gccgtgtgct cgccgtgctg
      601 acgggcgcgg tggtgctgtt gacggcggcg atgagtctga tccgcgacga ctaccactac
      661 ctgtggctgg gccttgttct gctggcctgc accgccgcct gcaatgacct ggcgaccgtg
      721 ccgtacaacg cgatgttgcg gcaggtctcg accctggaca gcccgggccg gatatccgga
      781 ttcggtttag ccatgggctt tctcggcagc gtggtgctgc tgctggtcgt ctacttcggg
      841 ttcatcgccg gccatggcga cgctcacgga ttgctgcagc tgcccgccgc cgacggccag
      901 aatgtgcgcg cggccgtcct gctggccgcc gtatggttcg ccctgttcgc gctgccggtg
      961 ttcatcgccg tgccgccgcc ggctcccaag cccgagggtc cgagggaggc ggtcgggttc
     1021 ctcggcacct atcgcacgct gtggtcggag atcaccggtg aatggcggcg cgaccgcaac
     1081 atcgtgtact acctgctcgc cagcgcggtg ttccgcgacg gactcaccgg cgtggtgacg
     1141 ttcggcgcgg tgctgggcgt caacgtctac ggcatctcgg gggcggacgt cctgttgttc
     1201 ggcgtaggcg ccagcgtgat cgcggccgtc ggcgcggtgt ccgggggtct gctcgatgac
     1261 cggctcggtt ccaagccggt catcgtcggt tcgttgatcg cgatgatcac cgtcgctgtc
     1321 acgctgatgg cgctgtccgg ggcgccggcg ttctgggtgt gcgggctgct gctgtgtctg
     1381 ttcatcgggc cgacccagtc gtcggcgcgc acgctgatgc tgcgcatgtc ggccgacggc
     1441 aaggagggca tggctttcgg cctctacacc acgaccggtc gcgcagtgtc gttcctggcg
     1501 ccatggttgt tctccagctt catagatctg ttcggcaccg accgcgccgg catgggcggg
     1561 ctatgcgtcg tcctcgtggc cggccttgtg gggatgctca tggtgcgagt accggggcgg
     1621 tcgttggcgc acacctcctc gagcgcctcg acgagcaagc cgcgatagtc gcccgtcggc
     1681 tgtttccagc gcaacgcctt actcctacgc cccggcgacc gggcgcgggc actgtcaact
     1741 tgagcaatcc gagggcgatt ttcgccggtt cgcctccgcg ttcaccaggt cgcgtgtgac
     1801 gtatgaggct tatgcggtta gcagaacgtc cacacgacct caagttgaca gtgccctttt
     1861 atggcttcac ttcgagtgaa aaagtcgagt tgatggccgg ttccgagcgc gttgtct
//
LOCUS       DASWBF010000206         4103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_279897,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000206 DASWBF010000000
VERSION     DASWBF010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279897"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..556)
                     /locus_tag="VF874_07350"
                     /pseudo
     CDS             complement(334..556)
                     /locus_tag="VF874_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854978.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(571..2367)
                     /locus_tag="VF874_07355"
     CDS             complement(571..2367)
                     /locus_tag="VF874_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX9175910.1"
                     /translation="MPSHASSRISKVLVANRGEIAVRVIRAARDAGLESVAVYAEPDA
                     DAPHVRLADEAFALGGQTSAESYLDFAKLLDAAAKSGANAIHPGYGFLSENADFAQAV
                     IDAGLIWIGPSPQSIRDLGDKVTARHIAARAQAPLVPGTPDPVKDADEVVAFAEEHGL
                     PIAIKAAFGGGGRGMKVAREIEEIPHLFDSAVREAVSAFGRGECFVERYLDKPRHVEA
                     QVIADQHGNVVVAGTRDCSLQRRYQKLVEEAPAPFLTDAQRKEIHESAKRICKEAHYY
                     GAGTVEYLVGQDGLITFLEVNTRLQVEHPVTEETAGIDLVLQQFKIANGEKLDLTEDP
                     TPRGHAIEFRINGEDAGRGFLPAPGPVSTFNPPSGPGVRLDSGVETGSVIGGQFDSML
                     AKLIVHGATREEALARSRRALDEFEVEGLATVIPFHRAVVSDPAFIGDGESFSVHTRW
                     IETEWNNTIESFTGGEPLDEETLPRQKVVVEVGGRRLEVSLPGDLALGNGAAPANPDV
                     IRKKPKPRKRGSHAAAGTASGDAVTAPMQGTVVKVAVEEGQEVSTGDLVVVLEAMKME
                     NPVTAHKDGTITGLAVEPGAAITQGTVLAEIK"
     gene            complement(2427..2870)
                     /locus_tag="VF874_07360"
     CDS             complement(2427..2870)
                     /locus_tag="VF874_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX9175911.1"
                     /translation="MPDCRSDKPRSVPPPLAEVVSDFQEVQGQDKLKLLLEFADELPP
                     VPRELDEAAMEPVPECQSPLFLHVDAADRGDVRLFFSAPAEAPTTRGFASILATGLDH
                     QPADDILAVPDDFYTDLGLAALISPLRLRGMSAMLARIKRHLRTA"
     gene            complement(2906..3790)
                     /locus_tag="VF874_07365"
     CDS             complement(2906..3790)
                     /locus_tag="VF874_07365"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX9175912.1"
                     /translation="MPLPADPSFELQSYAHPERLVTADWLASHLGAKGLAVVESDEDV
                     LLYDIGHIPGAVKIDWHTDLNDPHVRDYINGEQFAALMDAKGIARHDTVVIYGDKSNW
                     WAAYALWVFTLFGHPDVRLLDGGRDLWISSGRETTLEVPDRRSSGYPVVQRNDGEIRA
                     FKEDVLASLGEQPLIDVRSPQEYTGERTHMPDYPEEGALRGGHIPTAVSIPWAKAADD
                     SGRFRKREELSELYGFIQPDDKTIVYCRIGERSSHTWFVLTHLLGLPGVRNYDGSWTE
                     WGNTVRVPIVAGEEPGSP"
     gene            complement(3872..>4103)
                     /locus_tag="VF874_07370"
     CDS             complement(3872..>4103)
                     /locus_tag="VF874_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX9175913.1"
                     /translation="VRFAVPSAHELDAYVASGEPLKVAGGFTIDGLGGWFVDAVAGDP
                     SSVIGISLPLTRRLFERAGLSIPALWAANPVS"
BASE COUNT          640 a         1359 c         1408 g          696 t
ORIGIN      
        1 atggatgttg gacgcatcca gttcgccggt agcgccgacg acggtggcag cgccatgcat
       61 ttgggcgcgc aagcagacgc cttggccgaa gaagtggtcg ggtttgcccg acttccgcat
      121 cgcggaaagt gttggattct gaacagcagt cactgacgac ctcatcgttc gaatgtgtcg
      181 tgaaatcacg gatcgttcgg gctaatcagc cccacaagca aggagttgcg ccacccgaga
      241 cgttccgctc aggctgttaa ctcaaccttg ggcctgacag tactcccgcg ccgtgttggt
      301 cacaacgggc aatgatgatg ggcagccatc ttattagctt cttttgagcc ggctgtaaca
      361 cgtcgcggcg cgaatgtcat tcactgccaa ggcttccgcg gcgaaccgtc gcaccgattg
      421 ctcggccgcc gcgggcgcgg cggcgtggcc tgggcgcgct cgacaggtca cggacgcagt
      481 cgcggggtcg aagacgatct cggcctcgta gcgcgcgcag ataccaagcg ccggcgttga
      541 tttcgaccgg ctccatcgtg cgggaaaggc ctatttgatc tcggcgagga cggtgccctg
      601 ggtgatcgcg gcaccgggct ccacggcaag ccccgtgata gtgccgtcct tgtgggcggt
      661 gacggggttc tccatcttca tcgcctcgag cacgacgacg aggtcgccgg tgctgacctc
      721 ctggccctct tcgacggcga ccttgaccac ggtgccctgc atcggcgcgg tgaccgcgtc
      781 accggaggct gttccggcgg ccgcgtgcga cccgcgcttg cgcggctttg gcttcttgcg
      841 gatgacgtcc gggttggccg gcgcggcgcc gttgcccagc gcgagatcgc cgggcagcga
      901 cacctcgagc cggcggccgc ctacctcgac gaccaccttc tgccgcggca gcgtctcctc
      961 gtcgagcggc tcgccaccgg tgaacgactc gatcgtgttg ttccactcgg tctcgatcca
     1021 ccgggtgtgc accgagaagc tctcgccgtc gccgatgaac gcagggtcgg acaccaccgc
     1081 gcggtggaac gggatcacgg tggccaggcc ctcgacctcg aactcgtcga gcgcgcgccg
     1141 cgaccgggcc agcgcctcct cacgagtggc gccgtgcacg atcagcttgg ccagcatcga
     1201 gtcgaactgc ccgccgatca ccgaaccggt ctcgacgccg gagtccagcc gcacgcccgg
     1261 accgctcggc ggattgaacg tgctcaccgg accgggggcg ggcaggaagc cgcggccggc
     1321 gtcctcgccg ttgatccgga actcgatcgc gtgcccgcgg ggagtgggat cctcggtgag
     1381 gtcgagcttc tcgccgttgg cgatcttgaa ctgctgcagc accagatcga tgcccgcggt
     1441 ctcctcggtg accgggtgct ccacttgcag tcgagtgttg acttcgagga atgtgatcaa
     1501 gccgtcctga cccaccaggt actcgacggt gccggctccg tagtagtggg cctccttgca
     1561 aatccgcttg gccgactcgt ggatctcctt gcgctgagcg tcggtcagga acggtgcggg
     1621 cgcctcttcg acgagcttct gatagcggcg ctgcagcgag cagtcgcggg tgcccgccac
     1681 gacgacgttg ccgtgctggt cggcgatcac ctgcgcttcg acgtggcgtg gcttgtccag
     1741 gtagcgctcg acgaagcatt ctccgcggcc gaacgcggac accgcctcac gaaccgcgga
     1801 atcgaacagg tgcgggattt cctcgatctc gcgggccacc ttcatgccgc ggccgccgcc
     1861 gccgaaggcc gccttgatcg cgatcggcag cccgtgctcc tcggcgaacg ccaccacttc
     1921 gtcggcgtcc ttcaccgggt cgggtgtgcc gggcaccagc ggcgcctgcg cgcgggccgc
     1981 gatgtggcgg gcagtgacct tatcgccgag gtcgcggatc gactgggggc tcgggccgat
     2041 ccagatcagg cccgcgtcga taacagcctg cgcgaagtcg gcgttctccg ataggaagcc
     2101 gtagccgggg tggatcgcgt tcgccccgga cttggcggcg gcatcgagga gcttggcgaa
     2161 gtccaggtac gactcggcgg aggtctgacc cccgagcgcg aacgcctcgt cggccagccg
     2221 tacgtgcggg gcgtcggcgt cgggttcggc gtacacagcc acgctttcga gcccggcgtc
     2281 cctagccgcc cggatcactc ggaccgcgat ctcgccgcgg ttggcgacga ggaccttgga
     2341 gatccgtgag ctggcgtgac taggcactgc gcctcctacg tggcttgttc ctaaggaaag
     2401 tctttaagaa tgtatccgag ggcagtttag gcggtgcgca ggtgccgctt gatcctggcc
     2461 agcatcgccg acatgccgcg cagccgcaac gggctgatca acgccgccaa acccaggtcg
     2521 gtgtaaaagt cgtcgggcac ggccaggatg tcgtcggcgg gctggtggtc cagccccgtc
     2581 gcgaggatgg aggcgaatcc gcgggtggtg ggcgcctcgg ccggggcgct gaaaaacagc
     2641 cggacgtcgc cgcggtcggc ggcgtcgacg tgcagaaaaa gcggcgactg gcattcgggc
     2701 accggctcca tcgcggcctc gtcgagttcg cggggcaccg gcggcagttc gtcggcgaac
     2761 tccagcagca gcttgagctt gtcctgaccc tgcacctctt ggaaatcgga caccacctcg
     2821 gccaggggcg gcggcacgga gcgaggcttg tccgagcgac aatctggcac ggagcgaggc
     2881 ttgtccgagc gacaatctgg cacggtcatg gcgagccggg ctcctcgccc gcgacgatcg
     2941 ggacgcgcac agtgttgccc cactccgtcc aggagccgtc gtagttgcgc acccccggca
     3001 gtccgagcag atgcgtcagc acgaaccagg tgtggctgga gcgctcgccg atccggcagt
     3061 acacgatggt cttgtcgtcg ggctggatga acccgtacag ctccgagagc tcctcgcgct
     3121 tgcggaaccg cccgctgtcg tcggccgcct tggcccacgg gatcgacacc gcggtgggga
     3181 tgtggccacc gcgcagcgcg ccctcttccg ggtaatcggg catgtgcgtg cgctcgccgg
     3241 tgtactcctg cggggagcgc acgtcgatca gcggctgctc gccgagggac gccaggacgt
     3301 cctctttgaa tgcgcggatc tcgccgtcgt tgcgctgcac caccgggtag ccgctggatc
     3361 gccggtcggg cacctcgagc gtggtttccc gcccgctgga gatccacagg tcgcgcccgc
     3421 cgtcgagcag ccgcacatcg gggtggccga acagcgtgaa cacccacagc gcgtaggcgg
     3481 cccaccagtt gctcttgtcg ccgtagatca ccacggtgtc gtggcgggcg atgcccttgg
     3541 catccatcag cgcggcgaac tgctcgccgt tgatgtagtc gcgcacgtgc ggatcgttga
     3601 ggtcggtgtg ccagtcgatc ttcaccgcgc cggggatgtg gccgatgtcg tagagcagca
     3661 cgtcctcgtc ggactcgacc accgcgagac cttttgcgcc gagatgggac gccaaccagt
     3721 cggcggtgac caaccgctca gggtgcgcgt acgactgaag ttcgaagctg ggatcagcag
     3781 gcaatggcac gccatgagcc tactgcgccc tgcgaaaccg gagtttcgca ccggaagtgc
     3841 gaccagacgt gcgaaactcc ggtttcgcgg gttatgacac cgggttggcc gcccagagcg
     3901 cggggatcga caatcccgcg cgttcgaaga gccgccgggt cagcggcagg ctgattccga
     3961 tcacgctgga cgggtcaccg gcgaccgcgt cgacgaacca gccgccgagg ccgtcgatgg
     4021 tgaacccgcc ggcgaccttg agcggttcgc cgctggcgac gtaggcgtcg agctcgtgag
     4081 ccgatggcac agcgaaacgc act
//
LOCUS       DASWBF010000207         6883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1207381,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000207 DASWBF010000000
VERSION     DASWBF010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207381"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VF874_07375"
     CDS             complement(<1..485)
                     /locus_tag="VF874_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9175914.1"
                     /translation="MRYLLTHLDDAWELTLVHMRLSLIPMLLGLLIAVPLGAFVQRTR
                     LLRRLTTVTASIIFTIPSLALFVVLPLIIPTRILDEANVIVALTLYTTALLVRAVPEA
                     LDAVPAQVKDAATAVGYRPLTKMLKVELPLSIPVLVAGLRVVAVTNISMVSVGSVIGI
                     GG"
     gene            complement(489..1511)
                     /locus_tag="VF874_07380"
     CDS             complement(489..1511)
                     /locus_tag="VF874_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9175915.1"
                     /translation="MISFEGVTKTYPDGTVAVDDLTLDVPAGTLAVFVGPSGCGKTTS
                     MRMINRMIEPTSGTLTVDGADVTKVNPVKLRLGIGYVIQSGGLMPHQRVIDNVATVPV
                     LRGQSRRAARRAALDVLERVGLDPKLANRYPAQLSGGQQQRVGVARALAADPPILLMD
                     EPFSAVDPVVREELQTEMLRLQNELHKTIVFVTHDIDEAVKLGDKVAVFGPGGVLQQY
                     DEPAQLLSNPANDFVAGFIGADRGYRGLQFFGATGIPLQDIRRAGDWELVTNPDGSPV
                     GWRNADGLVAGGSLFKPDGTLRQALDAALSSPSGIGVAVDADGKVIGGVHAEDVIEAL
                     DKRRKP"
     gene            complement(1508..2443)
                     /locus_tag="VF874_07385"
     CDS             complement(1508..2443)
                     /locus_tag="VF874_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9175916.1"
                     /translation="MARARWRSAVVVVLAVLLAGCGSSNPLGGGPVSGDLKSITVGSA
                     DFPESKIIAEIYAQALEANGFTIRRQFGIGSRETYIPAVRDHSIDLIPEYTGNLLQYF
                     DSKTTATSPDAVVLALIKALPGDLAILTPSPAADTDTVAVTRDTATKWNLKTIGDLAA
                     HSAEVKFGAPSEFLSRTEGLPGLKAKYGLDISPGNFVAISDGGGPATVRALVNGTVTA
                     ADIFSTSPAIPQNDLVVLEDPKNNFLAANVMPLVSSQKMSDQLKTVLDAVSAKLTTAD
                     LIALNTAVSGNSGIDPDEAARKWVQANGFDKPIAK"
     gene            2520..2873
                     /locus_tag="VF874_07390"
     CDS             2520..2873
                     /locus_tag="VF874_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEX9175917.1"
                     /translation="MTSDRAVPAEPPPIDVPAHTTAGSSDAAAPPPKVPFTKTAAAWW
                     ALIAGVLILIVLLIFIAQNLEPTTIRFLGWQWNVSRGIAFLVAAICGSLITVLAGAAR
                     MIQLRRVAKKGLRGR"
     gene            complement(2966..4633)
                     /locus_tag="VF874_07395"
     CDS             complement(2966..4633)
                     /locus_tag="VF874_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEX9175918.1"
                     /translation="MLTLALIGFLGGLITGISPCILPVLPVIFFSGASTARRPYLVIA
                     GLALSFSVVTLVGSTLLALLHLPQDAIRWAALVFLVLIGVGLIFPAVEGLLERPFARI
                     PQRQIGTGGSGFGLGLVLGVLFVPCAGPVLAAIVVAGATGSIGLPTITLTVAFALGAA
                     LPLLVFALAGKRITERVNAFRRRQRTIRIAGGVAMILLAVALVFDVPAALQRTIPDYT
                     ASLQDKVDDADIQQKLNLGGIVTDQNRQLSNCSNGGRQLEDCGPAPDIRGITAWLNTP
                     NGAPVDIKQLRGKVVLVDFWAYSCINCQRSIPHLTSWYNTYRDNGFTVIGIHTPEYAF
                     EKVPENVASGADKLGITYPVALDNSFSTWTNYRNRYWPARYLIDANGTVRHIKFGEGD
                     YDVTEKLIRELLTDANPSVNLPTPTESADTPPQDIRTPETYLSVGKVVNYGDTEKYDE
                     GTAMFTYPPTQPADTFTLNGLWTLDYQGATAGTDTSEIRLNYHAENVYVVVGGTGTIT
                     VTRGGKSSTIRISGPPNSHQIVADESGTGQLEVRVSQGLQVFSITYG"
     gene            4765..5415
                     /locus_tag="VF874_07400"
     CDS             4765..5415
                     /locus_tag="VF874_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX9175919.1"
                     /translation="MRNPHYKTLALAGLSVVTVVGLSACSNDGSSTSSSPAPAAPMTT
                     SVAMTPAASALVGPGCSSYAQQVPTGPGSIEGMAQDPVAVAASNNPMLTTLTAALSGK
                     LNPNVNLVDTLNNGQYTVFAPTDEAFKKLPQSTIDSLKTDSDTLTKILTYHVVQGQAS
                     PDKVAGTHTTLQGATLNVTGMGDTLKVNDAGLVCGGVKTANATVYMIDTVLMPPAQ"
     gene            complement(5489..6526)
                     /locus_tag="VF874_07405"
     CDS             complement(5489..6526)
                     /locus_tag="VF874_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9175920.1"
                     /translation="MTTESLAGLDLGALDRHLQRIGIPRSGQLCAELISGGRSNLTFH
                     VSDDASAWVLRRPPMHGLTPSAHDMAREYRVVAALGGRPVPVAGAVTLCEDESVLGAP
                     FAMVEYVPGRVVRSADELEAVGDSAEIERCIDELIRVLSDLHAVDPASVGLADFGRAD
                     GYLERQVRRWAGQWQHVRLDDDDRDADVRRLHSALAEAVPPQSGTSVVHGDYRIDNTI
                     LDLDDAAIVRAVVDWELSTLGDPLSDAALMCAERDPAMDPILGMRSAWSSPSIPSADQ
                     LAQRYSELSGRPLAHWPFHMSLAYFKIAIIAAGIDFRRRMAGGADGPDRVGEVIAPLI
                     ASGLKAINSPE"
     gene            complement(6544..6690)
                     /locus_tag="VF874_07410"
                     /pseudo
     CDS             complement(6544..6690)
                     /locus_tag="VF874_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
BASE COUNT         1190 a         2221 c         2272 g         1200 t
ORIGIN      
        1 ccgccgatgc cgatcaccga accgaccgac accatcgaga tgttggtgac cgcgaccacg
       61 cgcaggccgg ccaccagcac cggaatcgac agcggcagct cgaccttgag cattttggtc
      121 agcggccggt agcccaccgc cgtggcggcg tctttcacct gtgcgggcac cgcgtccagc
      181 gcctcgggca ccgcccgcac caacagcgcg gtcgtgtaca gcgtcagtgc gacgatcacg
      241 ttggcttcgt cgaggatgcg cgtcgggatg atcagcggca gcaccacgaa cagcgccagc
      301 gacgggatcg tgaagatgat gctcgcggtc acggtggtaa gccgccgcag cagccgggtg
      361 cgctgcacga aagctcccag cggcacggcg atcagcagcc cgagcagcat cgggatcagc
      421 gacaaccgca tatggaccag cgtgagttcc caggcgtcgt cgaggtgcgt gagcagatac
      481 cgcatgcgtc agggctttcg ccgcttgtcg agggcctcga tgacgtcttc ggcgtggacg
      541 ccgccgatca ccttgccgtc ggcatccacc gcgaccccga ttcccgacgg cgacgacagc
      601 gcggcgtcca gcgcctgccg gagcgtgccg tctggcttga agagagagcc gccggcaacg
      661 aggccgtcgg cgttgcgcca accgaccggc gaaccgtcgg gattggtcac gagctcccag
      721 tcgccggcac gccgaatatc ttgcagcgga atgccggtcg caccgaagaa ctgcagcccc
      781 cggtaaccgc ggtcggcgcc gatgaagccg gcgacgaagt cgttggcggg gttggacagc
      841 agctgcgcgg gttcgtcgta ctgttgcagc acgccaccgg gcccgaacac ggccaccttg
      901 tcgccgagct tgacggcctc gtcgatgtca tgggtgacga acacgatcgt cttgtgcagt
      961 tcgttctgca accgcagcat ctccgtttgc agttcttcgc ggaccacggg gtcgacggcg
     1021 ctgaacggct cgtccatcaa caggatcggc gggtcggcag ccagggcgcg cgccacaccg
     1081 actcgctgct gctgaccgcc ggacagctgc gccgggtagc ggttggccag cttcgggtcg
     1141 aggcccacgc gctccaacac gtcgagcgcg gcgcggcgcg cggcccgccg cgattgcccg
     1201 cgcagcacgg gcacggtggc gacgttgtcg atcacccgct ggtgcggcat cagcccgcca
     1261 ctctggatga cgtacccgat gcccagccgc agcttgaccg ggttcacctt ggtgacgtcc
     1321 gcgccgtcga ccgtgagcgt gcctgaggtg ggctcgatca tccggttgat catccgcatc
     1381 gacgtcgtct tgccgcagcc ggacggaccg acgaagacgg caagtgtgcc cgccggcacg
     1441 tcgagggtca ggtcgtcgac cgcgacagta ccgtcggggt aggtcttggt gacgccctcg
     1501 aagctgatca tttggcgatc ggcttgtcga aaccgttggc ctgcacccac tttcgggccg
     1561 cctcgtccgg gtcgatgccg gagttgcccg atacggcggt attgagtgcg atcaggtctg
     1621 cggtggtcag cttggccgag acggcgtcga gcacggtctt gagttggtct gacatcttct
     1681 gcgagctcac gagcggcatg acgttggcgg ccagaaagtt gttcttcggg tcttcgagca
     1741 ccaccaggtc gttctgcggg atggccggcg acgtactgaa gatgtcggcg gccgtgacag
     1801 tgccgttgac aagcgcccgc acggtcgcgg ggccaccgcc gtcgctgatc gccacgaagt
     1861 tacccgggct gatgtccaga ccgtacttcg ccttgagccc gggcagcccc tcggtgcggc
     1921 tcagaaactc cgacggtgcg ccgaatttca cctcggccga atgtgctgca aggtcgccga
     1981 tggtcttgag gttccacttc gtcgcggtgt cgcgggtaac ggccaccgtg tcggtgtcgg
     2041 cggccggtga cggcgtgagg atagccaggt cgccgggcag cgctttgatc agcgccagca
     2101 cgacggcgtc cgggctggtg gcggtggtct tcgaatcgaa gtactggagc aggttcccgg
     2161 tgtattcggg tatcaggtcg atcgaatgat ccctcaccgc agggatgtac gtctcgcggc
     2221 taccgatacc gaattggcgt cgaatcgtga agccgttggc ctccaaggct tgtgcgtaga
     2281 tctcggcgat gatcttcgac tcgggaaagt cggcagagcc gacggtgatc gacttcagat
     2341 cgcccgacac cggaccgccg cccagcggat tggagctgcc acaccccgcc agcagaaccg
     2401 ccaagaccac gactaccgcc gaacgccacc gggctctggc catgcgcaat cctctcggct
     2461 gggcctgacc gaccctagcg aatgtttgat tctcactgcg aacaggcaaa gatgaagata
     2521 tgaccagcga tcgcgcggtg ccggccgagc cgccgcccat tgacgtaccg gcgcacacca
     2581 ccgccggtag cagtgacgcg gctgcgcctc cgccgaaggt gccgttcacc aagacggcag
     2641 cggcgtggtg ggcgctgatc gccggcgtgc tgatcctgat cgtccttctg atcttcatcg
     2701 cgcagaacct ggagccgacg accattcgct tcttggggtg gcaatggaac gtgtcgaggg
     2761 ggatcgcttt cctggtggcc gcgatctgcg ggtcgctgat cacggtgctc gccggggcag
     2821 cccgcatgat tcagctgcgc cgggtggcga agaaagggtt acggggacgc tagccggcgg
     2881 cacctgatgc tattcaaagt tgactctgcg tccagggcgc gaaagttcga gtggcgcaca
     2941 ccctcagcgc agaatcaacg cgtgctcagc cgtacgtaat cgaaaacact tgcagccctt
     3001 ggcttacccg cacttcgagc tgccctgtcc cagattcgtc ggcgacgatc tgatggctgt
     3061 tcggcggccc gctgatccgg atggtgctgg acttgccgcc ccgtgtcacg gtgatggttc
     3121 cggttccgcc gaccaccacg tagacgttct cggcgtgata gttcagccgg atttccgagg
     3181 tgtcagtgcc cgcagtcgcg ccctggtaat ccagtgtcca tagaccattc agtgtgaagg
     3241 tgtcggcggg ctgggtcggc gggtacgtga acatggcggt gccctcgtcg tatttctcgg
     3301 tgtcgccgta gttgaccacc ttccccacgg acagataggt ctccggcgtg cggatgtcct
     3361 gcggtggtgt gtccgccgac tcggttggcg tggggagatt gacggacggg ttggcgtcgg
     3421 tgagcagttc gcggatcagc ttctcggtga cgtcgtagtc gccctcaccg aacttgatgt
     3481 gccgcaccgt gccgttcgcg tcgatcaggt agcgggccgg ccagtagcgg ttgcggtagt
     3541 tggtccacgt cgaaaagctg ttgtccagcg cgacgggata cgtgatgccg agcttgtcgg
     3601 caccgctggc gacattctcg gggaccttct cgaacgcata ctcgggggtg tggatcccga
     3661 tgacggtgaa cccgttgtct cggtaggtgt tgtaccaact cgtgaggtgc gggatcgagc
     3721 gctggcagtt gatgcatgag taggcccaga agtcgacgag caccaccttg ccgcgcaact
     3781 gcttgatgtc caccggtgcg ccgttcggag tattcagcca cgccgtgatg ccacggatgt
     3841 cgggtgcggg gccacagtcc tcaagctgcc ggccgccgtt gctgcagttc gacaattggc
     3901 gattctggtc ggtcacgatt ccgccgaggt tgagcttctg ctgaatgtcg gcgtcgtcga
     3961 ccttgtcttg cagcgaggcg gtgtagtcgg ggatcgtgcg ctgcagcgcg gccggtacgt
     4021 cgaacaccag cgccaccgcg agcaggatca tcgccacgcc gccggcgatg cggatcgtgc
     4081 gctggcgccg tcgaaacgcg ttgactcgct cggtgattcg ctttcctgcc agcgcgaaca
     4141 ccagcagcgg aagtgccgcg ccgagcgcga aggcgacggt cagcgtgatc gtcggcagtc
     4201 cgatcgaccc ggtcgcaccg gcgacgacga tcgcggcgag caccggcccc gcacacggga
     4261 cgaacagcac accgagcacc aggccaagac cgaaaccgct tccacccgtg ccgatttggc
     4321 gctgcgggat gcgggcgaac gggcgctcca acagcccctc gacggccggg aagatcaggc
     4381 cgacgccgat gagcaccaga aacaccagcg ccgcccagcg aattgcgtcc tggggcaggt
     4441 gcagcagagc cagcagcgtc gacccgacga gggtgaccac gctgaagctc aatgccagcc
     4501 cggcgatcac caggtagggc ctgcgagcgg tgctcgcgcc cgagaagaag atcaccggaa
     4561 gcaccggcag gatgcacggg gagatcccgg tgatgaggcc gccgaggaag ccgatcaatg
     4621 caagcgtcaa catgcccctc attcggagcc gaccgccgct cggactgttt ttcttctaca
     4681 cccatccacc gttgcgttcg ctccgaatca ctcagcgacc ccggtccgag acggcgtccg
     4741 ccgtcgaata caaaggagtg atgaatgcgg aaccctcact acaaaaccct tgcactggca
     4801 ggactttccg tcgtaacggt ggttggcctg tccgcatgtt ccaacgatgg cagcagcacc
     4861 tcgtcgtcgc cggcaccggc cgctccgatg acgaccagcg tcgcgatgac gccggccgct
     4921 tcggcccttg tggggcccgg ctgctcgagc tacgcgcaac aggtgccgac cggtcccggt
     4981 tccattgagg ggatggcgca ggatccggtc gcggtggcag cgtcgaacaa cccgatgctg
     5041 accacgttga cggcggcgtt gtcgggcaag ctgaatccga acgtcaacct ggtggatacg
     5101 ctgaacaacg gccagtacac ggtgttcgcg ccgaccgatg aagcgttcaa gaagctgccg
     5161 cagtcgacca tcgattcact gaagaccgac tcggacacgc tgacgaagat cctcacctac
     5221 cacgtggtgc agggtcaggc gtcgcccgac aaggtggccg gcacgcacac gactcttcag
     5281 ggtgcgacgc tgaacgtcac cggcatgggg gacaccctca aggtcaacga cgctggtttg
     5341 gtgtgcggtg gcgtgaagac cgcaaacgca acggtgtaca tgatcgacac agtgctgatg
     5401 ccgccggctc agtgatcgcg gatcttccgt aggtaggaag acgcaaagcg cccgaaacac
     5461 cctggcattc gggcgcttta gcgtttcttc attccggtga gttgatggcc ttcagccccg
     5521 acgcgatcag cggggcgatg acctcgccta cccggtcagg tccgtcagcc ccgccggcca
     5581 ttcgccggcg aaagtcgatg cccgcggcga tgatcgcaat cttgaagtaa gccagcgaca
     5641 tgtggaacgg ccagtgcgcg agtggccggc ccgacagttc cgaatatcgt tgggccaatt
     5701 ggtcggccga cggaatcgat ggggacgacc aagcgcttcg catgccgagg atgggatcca
     5761 ttgcagggtc ccgctccgca cacatcagtg ccgcgtcgct gagggggtcg ccaagggttg
     5821 acaattccca gtccaccacc gcgcgcacga ttgccgcgtc gtcgaggtcg aggatcgtgt
     5881 tgtcgatgcg gtaatcgccg tgcacgacgg acgtcccgct ctgtggtgga actgcttccg
     5941 ccagagctga atgcagccga cgcacgtcgg cgtcgcgatc atcgtcgtcg agccggacgt
     6001 gttgccactg gccggcccag cgacggactt gacgttccaa atagccgtcg gcacgaccga
     6061 agtcagcgag gcctacggac gccggatcga cggcgtgcag atcggacagc acgcggatca
     6121 actcgtcgat gcaacgctcg atctcggcgc tgtcgccgac ggcctcaagc tcatcggcgc
     6181 tgcgcaccac ccgcccagga acgtattcga ccatcgcgaa cggcgcgccg agcaccgact
     6241 cgtcctcgca gagagtgacc gctccggcca ccggcaccgg cctcccaccc agcgccgcca
     6301 ccacccggta ttcgcgggcc atgtcgtgcg ctgagggcgt cagcccgtgc atcggcggcc
     6361 ggcgcagcac ccatgcggac gcatcgtcgc tgacgtgaaa cgtcaggttg gaccgcccgc
     6421 ccgagatgag ctcggcacac agttggccgc tgcgcgggat tccgatgcgc tggagatggc
     6481 ggtccaacgc gccgaggtcg agcccggcca agctctccgt cgtcaccgga tctgtatagc
     6541 gcctcagcgt gcgttcctcg gcagcaggtc ccatacgtgt tcggtgccgt tgacttccgc
     6601 gacggtgaac tcgcctctgc gtgagtagcg caggcgcgtc accgatgcgt agtcgatcgg
     6661 gaacaacagc agcttctccg tgccgaggat gcgggtaggg tcgagagctg gcgcggtgac
     6721 gaggatcgac ggtaggtctc gaagccaccg gtgaccgcag ggtttcctct ggggtccttt
     6781 acttcgtggc cctggtcgat ctttgttccg tttccagctc ccgcccgtca aaccgtgcat
     6841 gcggttctcc cgcacacggc ttaccgacgt cgttcaccgc cgg
//
LOCUS       DASWBF010000208         4410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_219836,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000208 DASWBF010000000
VERSION     DASWBF010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219836"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF874_07415"
     CDS             complement(<1..321)
                     /locus_tag="VF874_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEX9175921.1"
                     /translation="MGLTMSQRKAVTTTIATRYAADKAGKGRILDELCATTGWHRNHA
                     GKALKNATRPGVVKPRRPRPPQYGPKVGAALIFCWAVLGMPAGKRLAPVLAELVAILR
                     RFGEL"
     gene            complement(637..906)
                     /locus_tag="VF874_07420"
     CDS             complement(637..906)
                     /locus_tag="VF874_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175922.1"
                     /translation="MAGHQSPLDSASVALVGDSEQTSNALCRKEINLAEIQHQRPVVA
                     ETPTCALGEVMDVGRVQLAGSDDHDGAAVRVRAQAHTVAGELGEK"
     gene            complement(1013..1732)
                     /locus_tag="VF874_07425"
                     /pseudo
     CDS             complement(1013..1732)
                     /locus_tag="VF874_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727920.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
     gene            complement(1783..2217)
                     /locus_tag="VF874_07430"
     CDS             complement(1783..2217)
                     /locus_tag="VF874_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9175923.1"
                     /translation="MSKAENHATPVPHGQPVEFRVPARLESLAVIRALVTALGTLEDL
                     DLDAVADLRLALDEACTALIRSAAPDSRLTVTVRLREQDLWVSVSAPCVEENALRHGT
                     FSWHVISSLTDDVETFHDGAKVGDHGRVFGITMTARRLNTTR"
     gene            2704..3618
                     /locus_tag="VF874_07435"
                     /pseudo
     CDS             2704..3618
                     /locus_tag="VF874_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
     gene            complement(3612..3881)
                     /locus_tag="VF874_07440"
     CDS             complement(3612..3881)
                     /locus_tag="VF874_07440"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX9175924.1"
                     /translation="MTAYDPRLESIDRGWLSVPTASDPYVAAKDADAILVLTEWPEFA
                     ELDWAMIGRNAATGAVVIDTRNLVDANVTHDSDLAYLGNGRAGGY"
     gene            complement(3878..>4410)
                     /locus_tag="VF874_07445"
     CDS             complement(3878..>4410)
                     /locus_tag="VF874_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX9175925.1"
                     /translation="DGAHADRAADMVARLYADDTDTILPMTLESAELSKYASNAFLAV
                     KLWYVNSLAELCAGVGADITDVTGCMGADERIGPRFLAPGPGWGGSCLPKDTAALLHT
                     GQSHGIALREVESARSTNEAQRRRIIATLHRTLRCPLTEVRIAVLGLTFKAGTSDIGT
                     HPRSLSAPNLRGPVRR"
BASE COUNT          710 a         1487 c         1453 g          760 t
ORIGIN      
        1 cagctcgccg aagcggcgca ggatcgccac cagctcggcc agcaccgggg ccagccgctt
       61 gcccgccggc atacccagaa ccgcccagca gaagatcagc gccgcaccaa ctttcggccc
      121 gtactgcggc gggcgcggac ggcgcggctt gaccacccca ggccgcgtcg cgttcttcag
      181 cgccttgccg gcgtggttgc ggtgccagcc ggtagtagcg cacaactcgt ccagaatgcg
      241 gcccttgccg gccttgtccg ccgcgtagcg ggtcgcgatc gtcgtcgtca cggctttgcg
      301 ttggctcatc gtcagcccca tcgtccgggc ctaaccagcg atacctccac cgccgcgtag
      361 gcgcgccgta cgcgcgcatt ctcatgtgag gcaacgaacc tcgctacgcg cgcattttcg
      421 atgagtcaac gcgctgggtt gtcaccccca cggcgcgggg gtactgtcat agcaggggtt
      481 gagttcacag cctgagcgga gcgtctcggg cggcgtagga ctagtattcc ctgctcgtga
      541 ggattgcccg ggtgtttccg tcatcaacgc gacgcattcg aactgatggg gtcgtcaatg
      601 actgttgttc acaacgcaaa actctccgac gggcggtcac ttctcgccca attctccggc
      661 gacggtgtgt gcttgggcgc gcacgcgcac agcggcgcca tcgtggtcgt cgctgccggc
      721 gagctggacg cgtccaacat ccatcacctc accgagtgcg cacgtcggtg tctcagcgac
      781 gaccggccgt tggtgctgga tttcagccag gttgatttct ttgcggcaca gggcattaga
      841 cgtctgttcg gaatcgccga cgagtgcaac cgacgcggaa tccaatgggc tttggtgccc
      901 ggccacgccg tgacccggtt gctgcgtatc tgcgacagcg atgcgcgtct accggtggtg
      961 tcgtcgattg acgacgcggt gcaaggtttc ggctccgacc cgggcacgtg gcctattgca
     1021 gctcgtcacg aagtcgggct agcgtccgcg ccagcaggcg tgagacgtgc atctgcgaga
     1081 tgccgactcg ttcggcgatc tggctctgcg tcagcgattc gaagatcgca acacgatgac
     1141 ggttcgctcc cgttcgggta atgcgtccag gaacggccgc agcgcctcgt gattctcgat
     1201 gcggtccagg ttcgtgtcat ggctgccgag cgtctcggtc agtggcgggc tgtcctcgtc
     1261 gccgccgcac cgctgtccat cgacaacgtg ttgtaaccac tgccgacgat cagcgcctcg
     1321 accacctcgt cgtggttaag gtccagttcg gttgccagct cccagggcgt cggcgcacgg
     1381 cccagtcgct gcgacatctc ggcggtggcg ctgcccagct gcagatgcag tccttgagcc
     1441 gacgtgggac cttgaccgcc cagctgttgt cgcggaagtg gcggcggacc tcaccgatga
     1501 tcgtcggtac cgcgaacgag acgaagtcgc tgccgcactc gacgtcgaac cggttcaccg
     1561 cgttcaccag tccaagccgc gcgacctgcg cgatgtcttc gcgtggctcg ccgcgcccgt
     1621 cgaagcgatg ggcgatgtgc tccgccagcg gcatgcaccg gcggacgatc ttctcgcggg
     1681 ctcgctccca ttccaacgag tcgacggcca acgctttcag ttccctgaac atgtcgggta
     1741 cgtcggcgta ttcagaacgg gttcgcggtg acgaactttc tttcacctgg tggtattgag
     1801 ccgtcttgcc gtcatggtga tcccgaagac gcggccatga tcacccacct tggcgccgtc
     1861 gtggaacgtc tccacgtcgt cggtgagcga actgatgaca tgccaactga acgtaccgtg
     1921 ccgcagcgcg ttctcctcga cgcacggcgc cgacacggaa acccacaggt cctgctcccg
     1981 gaggcgcacc gtgaccgtca ggcgagagtc cggggcggcc gaccggatca aggcggtaca
     2041 cgcctcgtcg agggccaacc gcagatcggc caccgcatcg agatccaggt cctccagcgt
     2101 gccgagcgct gtcaccagcg cgcgaatcac ggcaaggctt tccaaccgcg cgggcacccg
     2161 gaactcgacc ggctggccat gcggcaccgg cgtggcgtgg ttctcagcct tggacacctg
     2221 gcctcctggc attggcacgg aatacccttc acccttttgc caccggtcac gacgatccgc
     2281 tcagacaagc atgaatgccc atgaaaaggg tattcccgcg gggtattccc gcgtcgtgca
     2341 ggaccgaccg gtcgccgacg gaccgcccac cgcgtcgccg cggtgttggg atggtcgtgt
     2401 tggcgctgtt ggcgttgggc gtctacgccg tggtgtttct tggacctcat gccgcagatg
     2461 caatgacccg tggtgcaccg gagacaccgt gccaccgctt cgaactcgtt ccatcaccat
     2521 cgggataccc ggcgtgaacc tgctgtaaac ggcgatgttt gaggcgtaga tctccgtgaa
     2581 agaaacaacg tcttaaggct gcgccgaagc actgtcgccg gccccggcgt gcggcccatc
     2641 cgccgaggtc gcggtttctc ctaatattga cgcgaacggc acgcccgtca cggatgccga
     2701 cacattggcc gcatcgaaga actggtcatc ccgccagcct ggaaaaaacg tctggatctg
     2761 cccgcatccc aatggccaca tccaggccgt cggcaccgac gcggctggcc gcaagcagta
     2821 cctctaccac caatgctggc agccggagcg cgccgaagag aagtacgacc ggaccttgag
     2881 cctggccaag cggttgccgg actggcggat gcaggtgctc ggcgatctgc gcgggcgcgg
     2941 actgcaccgc gaccgggtac tggcggtcgc gcaccagctg gtcgaccgcg gctacttccg
     3001 cgccggcggt gaagagtacg cgcaggagaa cggcagcttc gggctggcca ctctgcgccg
     3061 cgagcacgtc cgggtgcgca gcggcagcgt cgagttcgac taccccgcca agagcggggt
     3121 gcgccgatcg aactggagga cccgctggtc gtgcgcgcgg tgcggtcgct gatgcgcgcg
     3181 cagacccggc tgccgcggct gctggtgtac cgcagcgacg agggcggctg ggtggaagtg
     3241 cgcgccaacg acgtgaacgt gcgcttccgc gacctcgtgg gagacgagtt cagcatcaag
     3301 gacctgcgca cctggcacgg caccgtgctg gccgcggagg cgttcgcgca ctcccccgag
     3361 ccgacgtcga agaccgcacg gcggcgcgcc gagtcggcgg tgatgaaggt ggtcgccagc
     3421 gaactcggca acaccccggc ggtggcgcga gcctcctacg tcgacccgcg cgtggtgcag
     3481 gcctaccagg acggcctcac gatcgcgccg gcgatccagc gggcccagcg ccaccgctcg
     3541 ctgaccgcgc gccagcaact gatcgagaag gcgaccgcgc ggctgatcca ccgcgtcgag
     3601 aaggccgcta gtcagtagcc gcccgctctg ccgttaccca ggtaggccag atcggagtcg
     3661 tgagtcacgt tggcgtccac caggtttcgg gtgtcgatga cgacggcgcc ggtggcagcg
     3721 ttgcggccga tcatggccca gtccagctcg gcgaactccg gccactcggt gagcaccagg
     3781 atcgcgtcgg catctttggc ggcgacgtag gggtcgcttg cggtggggac cgaaagccat
     3841 ccgcggtcga tgctttccag gcgcgggtcg taggcggtca cctgcgcacc ggcccgcgca
     3901 agttcggtgc agacagcgag cgagggtgag tcccgatgtc gctggttcct gccttgaagg
     3961 tcaagcccag caccgcgatt cgcacctcgg tgagcggaca ccggagcgtt ctgtgcagtg
     4021 tcgcgatgat ccgcctacgc tgcgcctcgt tggtgctgcg ggccgactcg acttcgcgca
     4081 acgcgatccc gtgcgactgc ccggtgtgca gcagggcggc ggtgtccttc ggcaggcatg
     4141 atcctcccca cccggggccg ggcgccagga accggggccc gatccgctca tcggcgccca
     4201 tgcagccggt gacgtcggtg atgtcggcgc caacgccggc gcacaactcg gccagcgagt
     4261 tgacatacca cagcttcacc gcgaggaatg cgttgctcgc gtacttcgac agctcggcgc
     4321 tttccagcgt catcggcaga atcgtgtcgg tgtcatcggc gtacagtcgc gccaccatgt
     4381 cggcggcccg gtccgcgtgg gcaccgtccg
//
LOCUS       DASWBF010000209         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_219854,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000209 DASWBF010000000
VERSION     DASWBF010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219854"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..730)
                     /locus_tag="VF874_07450"
                     /pseudo
     CDS             complement(130..730)
                     /locus_tag="VF874_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139658.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(732..1298)
                     /locus_tag="VF874_07455"
                     /pseudo
     CDS             complement(732..1298)
                     /locus_tag="VF874_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005118249.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1324..1580)
                     /locus_tag="VF874_07460"
                     /pseudo
     CDS             complement(1324..1580)
                     /locus_tag="VF874_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
BASE COUNT          262 a          519 c          547 g          252 t
ORIGIN      
        1 aacgacggaa ggaccaccaa aacgtcgcgg gtacttctgt ccgcctcggg gttcctgcgc
       61 cgcgtcagcg gtaggcgcga gcaggacgag ccgcagaacg ccgcgctacc tgcctgacag
      121 cggatgggct cacgtccggg tccgggtgaa ccccttgttg cgcatcagga aatcggccag
      181 gtagccgtcg tgtgggatcc gaggggccgc agagtgggaa cggcgccgcg gtagacgttg
      241 gtgatggttg gctcggcgac caactgatcg atgagagagg ccgtcctcgc gtgaccggcc
      301 cgccacccac acgcagggga ctggcaactc gacgtcgatg gtgtccgcgt cgggccgagc
      361 caacacgtgc acggtccggc cgtagtggcg cgtagccgtc gccgaggtcg gcgataattt
      421 ggtcggcgcc gagcacggcc tgcgctcgta ccgccttccg acccagaaag tcggaaagag
      481 cgcgcgcatt cgcaatgggt gtcgttggca gagtggccat ttcgtcgacg ttcggcggtg
      541 gccgcagcgc cgccaaccgt tcaccgatgg cgtgtgcgag cggtgcgggg ttgccctcgt
      601 agagcacggc ggtggcgttg acgcacgcta tgctgcccag tccggcgatc gagtcgacga
      661 tcacgtcgag atgctcgcgc cagtcctgtt cggcgatgat caggatcttg gtgcggccgg
      721 gcccgttcac cggacacgcc acccaggccg cgcacgacct ggtcgatcgc agcgaccagg
      781 tgcggctgat cggtcgacgg cgacaacgcc gcccgccagt tcccggaatg agccagcgac
      841 ccgaccaaca gagacgcggt cttgccccac ccccaggatg ccggcggatc gggcaattcc
      901 agccagtccc aatgggtttc ctccccgggt ggatgcggga tcacggcgtc gggtcgctcg
      961 gtcgcgctcc ggcaggcctc gcagacccgg cgcaagttcc gttgcggatg ttgcgggtca
     1021 ggctctgata cgacagctcg aacccaaggt cctcgagttc gtcgaacagg gtgcgagccc
     1081 acagatgcgg gtcctcgacc aggcgggcgg tgacgtagtc gaccaacggg tcgaacgggt
     1141 ccggcgacgg ccgcgcccgc acacccggct tttgttcacc gttgagatac cggcggaccc
     1201 gttttgcggt cgaacccggt gtggcgggcg atcgccgaga tcgaccaacc acgtttgcgc
     1261 agggcatgta cttccacatc gtcctcccat gtgagcatga gaaagcgggc cgtgcgacac
     1321 caaggggccg ggcgccagga accggggccc gatccgctca tcggcgccca tgcagccggt
     1381 gacgtcggtg atgtcggcgc caacgccggc gcacaactcg gccagcgagt tgacatacga
     1441 cagcttcacc gcgaggaatg cgttgctcgc gtacttcgac agctcggcgc tttccagcgt
     1501 catcggcaga atcgtgtcgg tgtcatcggc gtacagtcgc gccaccatgt cggcggcccg
     1561 gtccgcgtgg gcaccgtccg
//
LOCUS       DASWBF010000210         3245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_148994,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000210 DASWBF010000000
VERSION     DASWBF010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148994"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..>3245
                     /locus_tag="VF874_07465"
     CDS             278..>3245
                     /locus_tag="VF874_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004018622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEX9175926.1"
                     /translation="MATRDVFSEEELARLRGFPEITRAELIRYFTLTEADETFVRQFR
                     GPANVLGASVQLCTLPWLGFVPDDVRAAPAVAVGRLAERIHVAAGELSGYAARGQTRT
                     DHLHALQRYSGWRLIDTSGWKELDEFLFARAMEHDSPKLLFGQACEYLSSGQVVRPGV
                     VHLLEHVATARTRARAETWTRVAHLLNADRCADLDVLLAVDPDLGRTALTWLGIGPTS
                     VSPAAVKAEVAKLTFLRRLDAHTMDLSMLPTERRRFLAGVGRRLTGQALSRREPERRY
                     PILLALLAQSAVDVLDEVLLLFDQAISGRESAAQAKLTELLAEHARRGEDRQRVLDEI
                     LQVVLDPEVADEAVGLLLRERVGMERMRAAWVAREQPLPRDHGHLAVLDASMSYLRQF
                     APTVLGAVRFAGGPGTEDLVYGVSVLSELYATGARKVPADAPTGFVPTRWAGYLTAAS
                     ETGDVTAYRHYWELCVLMALRDGLRAGDIYVPGSRRYADPISFLLTAEQWEPRRADFC
                     RVVGKPVCAADALAQADQELHTALADLDTQLATGEVGHVRLGDDGELIIPRLPAEDVP
                     AEVETLRDELAAMLPHLPIAAALVELDARTGFTDHLIHAGGKVARSPELKRNLLYVLI
                     AESTNMGLVAIAESAGVSYDVLAWTAEWYFRPETLAAANAAIVNYHHRLPHAQAFGAG
                     TLSSSDGQRFPVKGKSITARHLSRYFARGQGISTYSHVSDQHSTFDTKVIVATAPESH
                     YVLDGVLGNDTDLPIVEHATDTHGATLANFALFDLVGKQLSPRIRDLGKITLYRTGTA
                     QQFTARYPLAGPLLTRRLNTELITERWDDLLRVAASVHGGHATAALVVGKLCSSKRRQ
                     NTLTAAIKEYGALRRSVYAARYLADETYQRRIGRQLNKGENVHTLKRNLAYANEGALQ
                     RRHHEQQTEQMWCLTVVTNAIVCWATEYHGRAVDILRGQGRQIDSELLAHIWPTHHAN
                     VHFYGTHTIDIDGELAK"
BASE COUNT          541 a         1056 c         1094 g          554 t
ORIGIN      
        1 ctcgcgcgcc tgctcgccga cgcaggcctc aagctggcga tcaacgcaag tctgaaggtc
       61 gccgacgtag gaccggaact cgtcggtggt ccacctgttg acgaactcgc ggtagggctg
      121 cggcgtgcag cttgatgtgc cgcatcccgg ccaggcgtcg taggcccacc ggtagtttcc
      181 agcgaaaaaa caacgattac ggcgggcgga gccccgccga aggtcgtcgt taatatgctg
      241 acgttttaac tgtcgatgtc cgcgggggtg agttggggtg gcaactcgcg atgtgttctc
      301 cgaggaggag ttggcccgtc ttcggggttt cccggagatc actcgtgcgg agctgatccg
      361 ctacttcaca ctgaccgagg cagacgagac attcgtacgc caatttcgcg gtccggcaaa
      421 cgttttgggc gcatccgtgc agctgtgcac gttgccttgg ctgggatttg tacccgacga
      481 cgtcagggct gctccggcgg ttgcggtcgg tcggctagcg gagcggatcc acgtagcggc
      541 gggtgagttg tccggctatg cggcgcgggg acagacccga accgatcact tgcacgcgct
      601 ccagcgatac agcggatggc gcttaatcga tacttcgggg tggaaggaac tcgacgagtt
      661 cctgtttgct cgggcgatgg agcacgattc accgaaactc ctgttcgggc aggcatgcga
      721 gtacctgtcc tcgggacagg tggtgcggcc gggtgtggtg catctgcttg agcatgtcgc
      781 caccgcacga acccgggcgc gggcagagac ctggacccgg gtggcgcacc tgctcaatgc
      841 ggatcgctgc gccgatctgg acgtattgct ggcggtcgac cccgacttgg gcagaacggc
      901 gttgacctgg ctgggtatcg gaccaacgag cgtgagcccg gccgcagtga aggccgaggt
      961 ggccaagctg acgttcctgc gccgcctgga cgctcacacc atggatctgt cgatgctgcc
     1021 gaccgagcgc cggcgcttcc tggccggggt gggtcgacgg ttgacgggtc aggccctgtc
     1081 gcggcgggaa ccggaacgtc gttacccgat cctgttggcc ttgttggcgc agtccgcggt
     1141 cgacgtgctc gatgaggtgc tgctgttgtt cgatcaggcg atctccggcc gcgagtcggc
     1201 cgcgcaggca aagctgaccg agctgctggc cgagcacgcc cgccgcggcg aggaccgcca
     1261 gcgagtgctc gacgagatcc tgcaggtagt gctcgatccc gaggtcgccg acgaggcggt
     1321 gggcttgttg ctgcgcgaac gcgtcgggat ggagcggatg cgggcggcgt gggtcgcccg
     1381 cgagcaacct ttgccacggg atcatgggca cctcgctgtg ttggacgcgt cgatgagcta
     1441 cctgcgccag ttcgccccga ccgtgctggg cgcggtccgc ttcgccggtg gtcccggcac
     1501 cgaggacctg gtctatgggg tgtcggtgtt gagcgagctg tacgccaccg gggcgcgcaa
     1561 ggtgccggcg gatgcgccga ccgggttcgt gcccacccgg tgggccggct acctgaccgc
     1621 ggcatcggag acaggcgacg tcaccgccta ccggcactat tgggagttgt gcgtgctgat
     1681 ggcgctgcgc gacgggctgc gcgcgggcga catctacgtg cccgggtcgc gtcgctacgc
     1741 cgacccgatc tcgttcctgc tcaccgccga gcagtgggaa ccgcggcggg cggacttctg
     1801 ccgcgtcgtc gggaaaccgg tgtgtgcagc cgatgcgttg gcgcaagccg accaggagct
     1861 gcacaccgcg ctcgcggatc tagacacaca gctcgcgacc ggcgaggtcg gccacgtgcg
     1921 gctcggcgac gacggggagc tgatcatccc gcggctgccc gccgaggacg tgcctgccga
     1981 ggtcgagacg ctgcgcgacg agctggcggc gatgctgccc cacctgccca tcgcggcggc
     2041 gctggtcgaa ctcgatgccc ggaccggatt caccgaccat ctgatccatg cgggcggcaa
     2101 ggtggcccgc tcgccggagt tgaaacgcaa cctgctctac gtgctcatcg ccgaatccac
     2161 aaacatgggg ttggttgcga tagccgaatc cgctggcgtg tcctacgacg tgctggcttg
     2221 gaccgccgaa tggtacttcc gtcccgagac gctggctgcg gcgaacgccg cgatcgtcaa
     2281 ctaccatcac cggttgccgc atgcgcaggc gttcggggcc ggcaccctgt cgtcctcgga
     2341 cgggcagcgg ttcccggtga agggcaaatc gatcaccgct cggcacctgt cgcgctattt
     2401 tgcacgcggc caaggcatct cgacatacag tcatgtgtcc gatcaacatt cgacgttcga
     2461 cacgaaggtg atcgtggcca ccgccccgga gagccactat gtgctcgacg gggtgctcgg
     2521 caacgacacc gatctgccga tcgtggagca tgctaccgat acccatggcg ccaccctggc
     2581 gaactttgca ctgttcgatt tggtcggcaa acagctctcg ccccggattc gcgacctcgg
     2641 caagatcacc ctctaccgca ccgggaccgc ccaacagttc accgcccgct acccgctcgc
     2701 cgggccgctg ctgacccggc gcctgaacac cgagctgatc accgagcgtt gggacgacct
     2761 gctgcgcgtc gccgcgtcgg tgcatggggg gcacgccacc gccgcgctag tggtcggcaa
     2821 actgtgttcg tcgaaacgcc gccagaacac gctcaccgcc gcgatcaagg aatacggagc
     2881 gcttcgccga agcgtctacg ccgctcgcta tctcgccgac gagacctatc aacggcgtat
     2941 cggccgccag ctaaacaagg gcgagaacgt gcacactctg aaacgcaacc tcgcctacgc
     3001 caacgagggc gcgttgcagc ggcgccacca cgagcagcag accgaacaga tgtggtgcct
     3061 gactgtcgtc accaatgcga tcgtgtgctg ggccacggaa taccacggcc gcgccgtgga
     3121 cattctgcgc gggcagggac gccagatcga cagcgaactg ctcgcccaca tctggcccac
     3181 tcaccatgcg aacgtgcact tctacggcac gcacaccatc gacatcgacg gcgaactcgc
     3241 caaac
//
LOCUS       DASWBF010000211         4421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_220005,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000211 DASWBF010000000
VERSION     DASWBF010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220005"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VF874_07470"
     CDS             complement(<1..297)
                     /locus_tag="VF874_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175927.1"
                     /translation="MRKEQADEFEQELMQPLAKALRARLGDAGEVHVNNEPRLMPGDR
                     VEYAGWLDVVLNDARTEVIFRPLSREDFSAIWQWGKSGRFIFPIPQGGGNAGDSA"
     gene            380..511
                     /locus_tag="VF874_07475"
     CDS             380..511
                     /locus_tag="VF874_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9175928.1"
                     /translation="MGSALAEALLSKGHDVTVWNRTAYKTEPLVAAGAVVGCRIRKL"
     gene            complement(765..1036)
                     /locus_tag="VF874_07480"
                     /pseudo
     CDS             complement(765..1036)
                     /locus_tag="VF874_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562846.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1401..2741)
                     /locus_tag="VF874_07485"
                     /pseudo
     CDS             complement(1401..2741)
                     /locus_tag="VF874_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            complement(2800..3309)
                     /locus_tag="VF874_07490"
     CDS             complement(2800..3309)
                     /locus_tag="VF874_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HEX9175929.1"
                     /translation="MTQQNGGAVTGRRQAGPENTPRLRLKPKAPATGDVDGAWWPHSD
                     DLPKELPDLLAVLSVRLEAVTRVMYNPAEWVNAPEKLLTGGREVLLEGSRLQPPGTVE
                     VHGINRKKVVLLVVPHQMDADLAHDTLMVAAAPNNAATVDDLLMISTQDREASARAAA
                     TERRWTADD"
     gene            complement(3547..4350)
                     /locus_tag="VF874_07495"
     CDS             complement(3547..4350)
                     /locus_tag="VF874_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9175930.1"
                     /translation="MRRDVSAELEVEITAPTTLEFQIAVAPHPKTEMSESLSFVMDGN
                     LVQPLEISGVHGNRIHKLEAPVGTLKVDYAATIFGRNDPAPVTEYDLSMYLRPSRYAE
                     ADKFYGFAATEFGNYIDSTTLLEKVSSWVGTRLNYVPGSSDPIDGAVDTMLAGAGVCR
                     DFAHLVVALLRAVNVPARVVSVYAPGLYPMDFHAVAEAFVDGQWRVVDGTLLAPRQTL
                     VRIATGRDAADIAFLDNHKGAITLNKLTVTAIVDGDLPRDSIDQLVSIG"
BASE COUNT          738 a         1511 c         1399 g          773 t
ORIGIN      
        1 ggcgctgtcg ccggcattgc cgccgccttg tggaatcggg aagatgaatc ggccgctctt
       61 tccccattgc cagatcgcag agaagtcttc gcggctgagg ggccgaaaga tgacttcggt
      121 tcgtgcatcg ttgagcacga cgtcgagcca cccggcgtac tcgacccggt caccgggcat
      181 caggcgcggc tcattgttga cgtggacctc ccctgcatca ccaagcctcg cgcggagggc
      241 ctttgcgagc ggctgcatca gctcctgctc aaattcgtct gcttgttcct ttctcattcc
      301 cgctccttcg tcagccggtt gcacagcatc tagatcgtgt cacgcgggaa agcgcatcgc
      361 tgtcatcggc ctcggaaaca tgggcagcgc gctcgccgaa gcgctgctgt ccaaaggtca
      421 cgatgtcacg gtttggaacc gcactgcgta caagaccgag ccgcttgtgg cggcgggcgc
      481 cgtggtcggc tgcagaatcc ggaagctgta ggagtgaatg gtgccgacga acaacctgct
      541 gagcaggtcc agcgccgggt cgccgaggcg gtcttggacg cgcactgaca aggcaagccc
      601 acgtcatcgt cgacgacggg gcgcaggggc tcgtgcagcg gcggatccgc gggtcgtgga
      661 ggcctcttga tccttctccg tgcatgcggg gctgtctgtc cccggggtgt cgtcgtcaac
      721 tggttggggc cacgcggggt cggggagcgg aggaggccgg gctactagat gcgccaatgg
      781 ggttggacgc gattgatgtc gagagctcgg agtccggggt accaggggcg atgacagcgt
      841 ggtcaaggac ggctttgatg atggcctgct gacgttccag accaaggcca ggccattggt
      901 cgcgcaggat gccgcctgcg cctgccagtt cgtagaccgc ggtggtgtcg ggggccgcag
      961 cgatctcgcg gcgggtctgg gcaatgcggg cggtgatcgg gtcacgggca gcgatccatt
     1021 cccgcgctgt gatcgcggca ctgtcaactt gagcaatccg agggcgattt tcgccggttc
     1081 gcctccgcga ttcaacaggt cgcgtgtgac gtatgaggct tatgtagtta gcagaacgtc
     1141 cacacgacct caagttgaca gtgccgtgcc gagaccctgc accgcccttg cattgaggtc
     1201 cgcctttcgg ccgtacagcg tcgccaacgc gggacctact ttccacccga agcccggttc
     1261 gcctcccacg tctgccgtat ctgctgattg aggtcgtcga actcccgcgg cggcggaatg
     1321 tcctgtcggg cctggatcac atcgccggcg tgctggtcgg cctgactcgc ggtgacgggg
     1381 cagcccggtg tggcgactgc cgcggccgac ttcgacgtga cgtcgcggat cgcttgcacg
     1441 cggatgcgcg cgccgcggtg cggcaccaag gtgacgtgct tgaccctctg ccgttcgtca
     1501 gcggcggtcg tggtgcgtaa ctccacgcgc cgcagaactt cccgcagcac cacccgcatc
     1561 tcgaccatcg cgaacgtggc acccaggcat ctacggttgc ccccaccgaa cggcaaccac
     1621 ctcgtcgggc tcagcgtggc gccgaccatc cgttccgggt cgaaccgatc ggggtccggg
     1681 tacacctcag cgctcgcgtg caccagcccg atccccggaa cgaccatcac ccctgccggc
     1741 agccgatagc ccgccagctc caccggcttc gtcagcatcc gccccacgtc gaacaccacc
     1801 ggccggatcc gcaacgtctc cttgaccacc gcgtcgaggt actcatcccc ggccgggtca
     1861 cccgccgcgc tggcctcggc cgcccgcacc gcgttggcca gcaccgccga gtgccgggtc
     1921 agtcgctcca gcgcccacga catacccgtc gcggtcgtct catggccggc gacgagcaac
     1981 gtcatcagct gatcgcgcaa ttcacggtcg gtcatcgcgc ggccgtcgtc gtcggcggcg
     2041 cggaccagca tcgccagcac gtcggtgcgc tcggccaggt tcggatcggc gcgacgctcg
     2101 gcgatctcgg cgtacagcag ccgatcggct tgcatcatgc gccgacgcat cccccgccag
     2161 gggcggcgac gctgcagatc cgggttcgcg acggccagcg tctcccacgg acccacgttg
     2221 agcagcttcg gcatcacctg gcgcaacgcg gcgagccggg ccgggtcgct ggcgccgatg
     2281 accgtccgca gaatcacctc cagcgtgatc cccgacattc ggggcgccac cgggaactcc
     2341 gtgcccaccg gccagccggc gatattcccc gcggcgatct cggccatcac cgccgcctgg
     2401 cgggcgaccg cgtcgcgctg aaacgacggc agcatcagac ggcgccgatc ccggtgcacg
     2461 tcgtcgtcga tcaccaacac cgaactgtca ccgagcatcc cgctcaacat cgaattcgct
     2521 tcgccggcat ggaaaatgct cggatcaccg gcgaacaccg ccttgatgtc ggcgggatcg
     2581 gccagataca cccatctgcc catcgatgcg atccgcagcg tgaacacact gccgtagcgg
     2641 cgtcgacacg ccgccacaaa acgcggccag tagcgcagca tcagtgccgc ctggacccac
     2701 ggaggcagcg caggaccggg aggcaacggg gtctcgctca tcacccgagc ctacgagcga
     2761 cagcggcgcg agcgtcggac ggccgccagc cgaagtcgtt cagtcgtctg cggtccagcg
     2821 ccgttcggtg gcggccgccc gggcgctcgc ttctctgtct tgggtgctga tcatcagtag
     2881 gtcgtcgacc gtcgcggcgt tgttcggggc cgccgcgacc atcagtgtgt cgtgtgcaag
     2941 gtccgcatcc atctgatggg gaaccaccag caggacgacc ttcttgcggt tgattccgtg
     3001 tacttcgacg gtgccgggcg gttgcaggcg agatccctcg agtaggacct cccgaccgcc
     3061 agtgagaagc ttttctggtg cattcaccca ttcagcgggg ttgtacatca cccgagtgac
     3121 ggcctccagg cgcaccgata gcaccgccaa cagatctggc agttccttcg gaaggtcgtc
     3181 actgtggggc caccacgccc catcgacatc accggttgcg ggcgccttcg gcttcaatcg
     3241 cagccgcggc gtgttctcgg gtcctgcttg gcgccggccg gtgacggcgc cgccgttttg
     3301 ttgtgtcatc cgacaatccc gtcttggccg cgtacggccc aatcaatcat atcggcgacg
     3361 acacaatatc agatggtcgc gtgcgacgta ccgccgatgc cgctgaccct actcgcattg
     3421 catcccggac acccttgaaa gcaaccgttg cgcgacatgc gcctggtgcg acccgtcatc
     3481 aatgtggtga aagtcgttgc acgtcagcac tgttcggcat tcggcacctg gacggccgcg
     3541 cgagccctat ccgatcgaca ccagctggtc gatcgagtcc ctgggcaggt caccatcgac
     3601 gatcgccgtc accgtcaact tgttcagcgt gatcgcaccc ttgtggttgt ccaggaaggc
     3661 gatgtcggca gcatcgcgtc cggtggcgat gcgcaccaag gtctgtcgcg gcgccaacag
     3721 ggtcccgtcg accacccgcc attgcccgtc gacgaacgcc tcggcgacgg catggaagtc
     3781 catcgggtac agacccggcg cgtacaccga aaccacccgg gccggcacat tgacggccct
     3841 tagcagcgcc accaccaggt gcgcgaagtc gcggcacacc ccggcgccgg cgagcatggt
     3901 gtcgacggct ccatcgatcg ggtcgctgga tcctggcacg tagttcagcc gggtgcccac
     3961 ccacgaggac accttctcca acaacgtcgt cgaatcgatg tagttaccga attcggttgc
     4021 ggcgaatccg tagaacttgt cggcttccgc gtagcggctg ggacgcaggt acatggacag
     4081 gtcgtattcg gtcaccggcg ccgggtcgtt ccggccgaag atcgtcgcgg catagtcgac
     4141 cttcaaggtg cccaccggag cctcgagctt gtggatgcgg ttaccgtgca caccgctgat
     4201 ctccaagggc tgaacgagat ttccgtccat cacgaacgac aacgactccg acatctcagt
     4261 cttcggatgc ggcgcgaccg caatctggaa ctccaacgtc gtgggcgcgg tgatctcgac
     4321 ttcgagctct gcgctcacgt ctcgcctcat agcgccgatg ataagggcca gcgaccaaaa
     4381 ccacgaagtg tgcgaccctc gcgaccgctg gtcagcgact c
//
LOCUS       DASWBF010000212         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1068997,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000212 DASWBF010000000
VERSION     DASWBF010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068997"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..244
                     /locus_tag="VF874_07500"
                     /pseudo
     CDS             1..244
                     /locus_tag="VF874_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357701.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            408..1616
                     /locus_tag="VF874_07505"
     CDS             408..1616
                     /locus_tag="VF874_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9175931.1"
                     /translation="MRDPSRVRVGGPLRRYAGGFREELEGQGYSPSTVVLHLQLMGHL
                     SGWLAGEGLSGAALTGAQVDRYLAGRRSAGLACHRTRRSLLPLLGYLGGLGVLSAPAV
                     SEPVPEVLAGYADYLRYERGLADSTIRRNVELVEPLVATLASGGEFGWERLDGAAVSA
                     FVVAHCRGRADGGGSPARMFTALRSLLRFVHLAGLVGTPLVDAVPSVPCRRLAGLPKA
                     LSVEQVAALLGSCDTGTMVGRRDRAIITMLARLGLRAGEVAALRLDDIDWRNGEITLR
                     GKGNRSERLPLPVDVGRAVVAYLTAERPATTTHRELFGRVRAPHGPLTRLAVTQLVAR
                     AAGRAGLAPVYAHRLRHTAATEMLRRGGSLTEIGQVLRHRRALTTAVYAKVDIEALRL
                     LARPWPGGVA"
     gene            1613..2545
                     /locus_tag="VF874_07510"
     CDS             1613..2545
                     /locus_tag="VF874_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9175932.1"
                     /translation="MSTPADLEQALADYLQVRRASGAGLARAEKLLRQYLSHLSEHGT
                     DRITVADAAAWATLPAAADPRWWAYRLSVARGFAAWLHTFDESVQVPPAGLIRSGPRR
                     STPYLYSDDEIVALLDAAGRLRHPLKSATYQTLLGLLAVTGMRVGEAIGLDRADFDAA
                     QGLLTVRAGKFGKARLLPLQPSTVTAVNGYLRDRDRLCPVVASDALLVSVYGNRLRYN
                     TVWRVVHKLLTDAGLTAGSPSRRRIHDLRHSFAVATLSDWYAHGADVAELLPRLSTYL
                     GHTDPAHTYWYLSAAPQLLTPAVRRLDTPQRGRR"
     gene            2542..3531
                     /locus_tag="VF874_07515"
     CDS             2542..3531
                     /locus_tag="VF874_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9175933.1"
                     /translation="MTALAPTLQAFFTDRLIRQRRVSSHTVAAYRDTFKLLLGFAERR
                     TGATPTRLDIADLDAPLIGEFLDHLQRDRGNSVGTRNARLAAVHSLFRYAATVHPEHA
                     ATIQRVLAIPPKRGDQPVITYLTDTEAEALLAAPDQRTWTGRRDHALLALAIQTGLRV
                     GELTALTRTDVHLGRAAHVGCRGKGRKHRITPLTGEVQRTLQAWLAENPGQPADPLFL
                     SRRGGPLSSDAVTQRLATHTATAARYCPTLAGKTVTPHVLRHTAAMRLLHAGVDTTVI
                     ALWLGHEKVSTTQIYVHADLALKEQALARTTPLNTQAGRYQPPDRLLAFLEAL"
     gene            3613..3754
                     /locus_tag="VF874_07520"
                     /pseudo
     CDS             3613..3754
                     /locus_tag="VF874_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          578 a         1282 c         1287 g          607 t
ORIGIN      
        1 catggcgctc gcggccgagc aggccgctgc ggccggtgag ccgttggcgg tcgcattgga
       61 cggcaagagc ctacgcggtg cacgccgcgc cggagccgcg gccgcgcatc tggtgtcggt
      121 gttcgcccac cgcgcccggc tggtgctcgg gcagctcgca gtggccgaga agagcaacga
      181 aatcccctgc gtgcgaaaga ttctccaccc cgtccggcag gtgcggctgc tggtcacggt
      241 ggacgtctta tgtcgatatc gacataatgc gtcttatgcc gaggtggctg ttatgccgat
      301 atggcggtga ttgcgctggt ccgggcggtg ctcggggtgt tgatatcggc ataataatcg
      361 gggtcgatcg ggcctacttc tcctgtcggt agtgagagga gaaggttatg agggatccat
      421 ctcgggtccg ggtcggtggc ccgctgcgtc gttatgcggg gggatttcgc gaagagcttg
      481 aggggcaggg gtattcaccg tcgacggtgg tgctgcacct gcagctgatg gggcatttga
      541 gcggctggct tgccggcgag ggtctcagcg gtgctgcgct gaccggggcg caggtggacc
      601 gctacctggc tggacggcgc agcgcgggct tggcctgtca ccgcaccaga cggtcgttgc
      661 tgccgctgct ggggtatctg ggtgggttgg gtgtgttgtc tgcgccggcg gtgtctgagc
      721 cggtgccgga agtcttggcg ggctacgccg attacttgcg ttacgaacgg gggcttgccg
      781 attcgacgat ccgacgcaat gtcgagttgg tcgagccgtt ggtggcgacg ctggccagcg
      841 gcggcgagtt cgggtgggag cggctggatg gggctgcggt gagcgcgttc gtcgtcgcgc
      901 actgccgtgg ccgtgcggat ggcggcggat cgcccgcgcg gatgttcacc gcgttgcggt
      961 cgctgctgcg gttcgtgcac ctggctggat tggtcggcac accgctggtc gatgcggtgc
     1021 cgtcggtacc gtgccggcgg ctggcggggc tgcccaaggc gctgtcggtc gagcaggtcg
     1081 cagccctgtt gggttcgtgt gacacgggca cgatggtggg gcgtcgggac cgggcgatca
     1141 tcacgatgtt ggcccggctc gggctacgcg ccggcgaggt cgccgccctg cggctcgacg
     1201 acatcgactg gcgcaacggt gaaatcacct tgcggggcaa gggaaaccga tccgagcggc
     1261 tgccgctgcc ggtcgatgtc gggcgcgcgg tcgtggcgta tctgaccgct gagcgccccg
     1321 ccacgacgac tcatcgcgaa ctgttcggca gggtgagggc tccgcacgga ccgttgaccc
     1381 ggcttgcggt cacccaactg gtggcccgcg ccgcgggccg cgcgggattg gcgccggtct
     1441 acgcgcaccg gctgcggcac actgcggcca ccgagatgct gcgccgtggc ggatcgttga
     1501 ccgagatcgg gcaggtgctg cggcaccgtc gggcgttgac caccgcggtc tacgccaagg
     1561 tcgacatcga ggcgctgcgc ctgctggccc gcccctggcc gggaggtgtg gcatgagcac
     1621 gccagcagac ctcgagcagg cgctggccga ctatctgcag gtccgccgcg cgtctggggc
     1681 cgggttggct cgggcggaga agctgttgcg ccagtacctg tcgcacctca gcgaacacgg
     1741 cacggaccgg atcacagtcg ccgatgcggc ggcgtgggca acgctgccgg ctgccgcgga
     1801 tccgcggtgg tgggcgtatc gactgtctgt ggcaaggggt ttcgccgcct ggctgcacac
     1861 tttcgacgag tcggtgcagg tgccgccggc ggggttgatc cgcagcgggc cgcgccgcag
     1921 caccccgtat ctgtactccg acgacgagat cgtcgcactg ctggatgcgg cgggccggct
     1981 gcggcatccg ttgaagtccg cgacctacca gacgctgctg ggtctgctgg cggtcaccgg
     2041 catgcgggtc ggcgaggcga tcggcctgga ccgcgccgat ttcgacgccg cgcaggggct
     2101 gttgaccgtg cgtgcaggca agttcggcaa ggcccgactg ctcccgctgc agccgagcac
     2161 ggtgaccgcg gtcaacggct atctacggga ccgagatcgg ctctgccccg tcgtcgccag
     2221 cgacgcgttg ctcgtgtcgg tctacggaaa ccggttgcgc tacaacaccg tctggcgtgt
     2281 ggtgcacaaa cttctcaccg atgccgggct caccgccggg tcgccatcgc ggcgacggat
     2341 tcatgacctg aggcatagct tcgcggtggc gacgctgtcg gactggtacg cccacggcgc
     2401 cgacgtggcc gagctgctgc cgcgattatc gacctatctc ggacacaccg accctgccca
     2461 cacctactgg tacctgtccg cggcgccgca gctgctgaca ccggccgtgc ggcgactcga
     2521 caccccgcag cgaggtcgac gatgaccgcg ctcgcaccga ccctgcaggc attcttcacc
     2581 gaccggttga tccggcaacg ccgggtcagc agccacaccg tcgcggccta ccgggacacc
     2641 ttcaagctgc tgctgggctt cgccgaacgc cgtaccggag ccacgccgac ccggctcgac
     2701 atcgccgatc tggatgcgcc actgatcggg gagttccttg accacctcca gcgggaccgg
     2761 ggcaacagcg tgggcacccg caacgcccga ctggccgccg tgcactcgct gttccgctac
     2821 gcggccacag tgcatcccga acacgccgcg acgatccaac gcgtgctggc gatcccaccc
     2881 aaacgcggcg accagccggt gatcacctac ctcaccgaca ccgaagccga ggcgctgctg
     2941 gccgcgcccg atcagcgcac ctggaccggc cgacgcgacc acgccctgct ggcactggcc
     3001 atccagaccg gactgcgggt cggcgagttg accgcactca cccgcaccga cgtgcacctg
     3061 ggccgagcag cccacgtcgg ctgccgcggt aagggccgca agcatcgcat caccccgctc
     3121 accggcgaag tccaaagaac cctgcaagcc tggctcgccg agaacccggg ccaaccggcc
     3181 gacccgctgt tcttgagccg ccgcggcggc ccgctgagct ccgacgcagt cacacaacgc
     3241 ctcgccaccc acaccgcgac agccgcgcgt tactgcccaa ccctcgccgg aaaaacggtc
     3301 acaccgcacg ttctacggca caccgcggcg atgcgtctcc tgcacgccgg ggtcgacacc
     3361 accgtgatag cgctgtggtt gggacacgaa aaagtttcca ccacacagat ttacgtgcac
     3421 gccgacctcg cgctcaaaga acaggccctc gcccgaacca ccccgctcaa cacccaagca
     3481 ggccgctacc agccaccgga tcggctcctc gccttcctcg aagcgctctg attatgccga
     3541 taccaacacc ccgagcaccg cccggaccag cgcaatcacc gccatatcgg cataacagcc
     3601 acctcggcat aagacgcgat gcacacccag accgcgaccg cgacactgat ctgcggcacc
     3661 ctgaaatcgc actacctaat ggtcgtcaag tcaaaccagg ccaagctgct ggcccggatc
     3721 accgcactcc cgtgggtcga ggtcccggtt gtct
//
LOCUS       DASWBF010000213         5454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1003497,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000213 DASWBF010000000
VERSION     DASWBF010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003497"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..988)
                     /locus_tag="VF874_07525"
     CDS             complement(122..988)
                     /locus_tag="VF874_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9175934.1"
                     /translation="MQTIDLAYVGRGLHEELVAHIADQEGYYEDEGVHVAIRDGVGWK
                     AERLRRGAMIGLGRALQSRLTDGISWTAPSVNTDRPLFWFLGSADVTSMADLRGRRLA
                     VHAAHTPPACFARIVLRKHGLDPDHDLKCLERHPGDYQMDLRHLRDGSIDAAYVGSTL
                     SPEQVAAEEGFHLLAWVGDHFQIPTVGVAVDPVHIPLDSPALQALVRANQRALRTLAE
                     QPSLAVDYIARFLDRLTRDEAQQYYERYVGPYFTPDRQVDLSIAQQAIAAVAAELGVA
                     AVAADAIYGPSA"
     gene            complement(1042..1785)
                     /locus_tag="VF874_07530"
     CDS             complement(1042..1785)
                     /locus_tag="VF874_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9175935.1"
                     /translation="MGRVAVITGASRGIGAATALVLAEQGFRVVVNYRSSAEQADEVV
                     RAATTAGGEAVAIRADVTAPDDVAAMVDETQRRWGRVDVLVHNALIPFDVTSFADLRW
                     EQLGGKLDRELHAAFLITKAVVPGMISRGYGRLVYLSTGLSRRPREGMITLGTAKAAL
                     DQFVRYVALELAPHGITANLVAPATVAETKVSEQLAAGQVGQLGATNPMGRLVRPTEV
                     AATVAFLASEDSGFTTGHYLEVNGGLAMD"
     gene            2631..3641
                     /locus_tag="VF874_07535"
     CDS             2631..3641
                     /locus_tag="VF874_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9175936.1"
                     /translation="MPSKTPKPPVDPVRWQPPPVDPLPDFGPADLTVVPVPGNAPEDV
                     VVDAAGNIWTGVDDGRIVRISPDGQPTVVGETGGRPLGLAVARDGRLLVCDSPRGLLA
                     MDTDSGKFETLVEEVDGRHLQFCSNVTETPDGTIYFTESTSAFTYPHFKGAIMEARPR
                     GSLFRRDADGTVLTVVPGLYFANGVTQTADGSALVFAETQGRRLSKYWLTGPQAGAVT
                     TLVANLPGYPDNISTGADGRIWVALVSPVNAAVEWLAPRAPALRKLLWKLPDRLQPQI
                     KPEVWAVAFDPDSGEAIAGLHSKHPSFGMVTGLVESDGKLWMGCIGAPAVAYCAIPPR
                     SD"
     gene            3641..3940
                     /locus_tag="VF874_07540"
     CDS             3641..3940
                     /locus_tag="VF874_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175937.1"
                     /translation="MTRDRLRRTNDGYYGDGYWLIDNRTNGLVLGEQIARGVNIGYHL
                     DAVEEWLRDMAQGRSSHVSPAGFVSVPWCNDIRNAGQVRRRRPCGTDDGSPRTGC"
     gene            complement(4243..4692)
                     /locus_tag="VF874_07545"
     CDS             complement(4243..4692)
                     /locus_tag="VF874_07545"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX9175938.1"
                     /translation="MDARSGPGLNDPFEEAIDDALSSLPADLHAAMSNVEIVVEDEPP
                     DRQPLLGLYRGVPLPRRSSTYSGVLPDTISIFGGPITRLAAGDADRLRREVRHVVLHE
                     IAHHFGISDERLSGIRHATSNSQALATSTRLVSRTSAANPPSRQCRP"
BASE COUNT          964 a         1802 c         1774 g          914 t
ORIGIN      
        1 aattcgaaac agaacagacc cggctctgaa accgggttac tcagtaacgg gatctcgcca
       61 gccaatcggc cgtcaagctg gccagacaca gaccagtcgc gagttcggac agtgctgacg
      121 ttcatgcgga cggcccatag atggcgtcgg cggccactgc ggcgacgccc agttcggccg
      181 caaccgcagc gatcgcttgc tgcgcgatgc tgaggtccac ctggcggtcg ggggtgaagt
      241 acgggccgac gtagcgttcg tagtactgct gcgcttcatc gcgggtgagt cggtcgagga
      301 accgggcgat gtagtcgacg gccaggctgg gttgttcggc aagggtccgc agcgcccgct
      361 ggttggcccg taccagcgct tgcagggcgg ggctgtccag tgggatatgg acggggtcca
      421 cggcgacgcc cacggtgggg atctggaagt gatcgccgac ccaggccagc aggtggaacc
      481 cttcttccgc ggcgacctgt tcgggtgaga gcgtgctgcc tacgtaggcg gcgtcgatgg
      541 agccgtcccg aaggtgtcgc aggtccattt gatagtcgcc gggatgccgc tcgagacatt
      601 tcaggtcgtg gtcggggtcc aggccgtgct tacgcagcac gatccgggcg aagcaggccg
      661 ggggcgtgtg agcggcgtgg acggccaggc gccgcccgcg aagatcggcc atggatgtca
      721 catcggcgct gccgagaaac cagaacagcg gacgatcggt gttgacgctg ggcgctgtcc
      781 aactgatgcc gtcggtcagc cgcgactgca gggcccgacc cagaccgatc atcgcaccgc
      841 ggcgcagacg ctcggctttc cagccgaccc cgtcgcggat ggcgacgtgg acaccttcat
      901 cctcgtaata gccttcctgg tcggcgatat gggcgaccag ctcctcgtgg agaccgcgcc
      961 cgacataggc cagatcgatc gtctgcacag ccaaaacccc cttccccaat ctggtgactt
     1021 ccctagccgt ggaacatggt ttcaatccat cgcaaggccg ccgttgacct cgaggtaatg
     1081 gccggtggtg aaaccggaat cctcactggc caagaacgcc accgtcgcgg ccacctcggt
     1141 ggggcgcacc aggcgcccca tcgggttggt ggcgccgagt tgacccacct gtccggccgc
     1201 taactgctcg ctgacctttg tttcggccac cgtggcgggc gcgacgaggt ttgcggtgat
     1261 cccatgtggt gccagctcga gtgcgacgta ccggacaaac tggtccagcg ccgccttggc
     1321 ggtgcccagc gtgatcatgc cctcgcgcgg gcggcgggac aaccccgtgc tgagatagac
     1381 cagtcggccg taaccgcggg aaatcatccc cggcacaacg gctttggtga tcaggaaggc
     1441 ggcatgcagc tctcggtcca gcttgccgcc gagctgctcc cagcgcagat cggcgaacga
     1501 ggtgacatca aatgggatca atgcgttgtg cacgagcacg tcgacccggc cccaccgccg
     1561 ttgggtttca tcgaccattg cggcgacatc gtcgggtgcg gtgacatcgg ctcgaatcgc
     1621 caccgcctca ccgccagccg tggtcgcggc ccggaccacc tcgtctgctt gctcggcact
     1681 ggagcggtag ttgacaacca cccgaaaccc ctgctcggcg aggaccagtg cggtcgccgc
     1741 accgattccc cgactggctc cggtgatcac tgcaacacga cccatcagcg ttgcctccgt
     1801 ctgtatctac cgtctgggta gcttgccgaa tgtgatagtt ccctgacgcc aaacgcaggg
     1861 acgatctcga cgtcatcgaa cgccgaaaat tcctgacaag ctgtcaagac gacgtgtgct
     1921 ggcgggggcc taacgctacg cctgcgatac acctcggtaa acgacacttg cgatacacct
     1981 cggtaaacgg tggcaccgat tacgtgcagg acgtttagaa cttcaggctg acagggacca
     2041 cgatcacgat cgcaccatcg atccaggctc cggttgctcg cggccacagc ggcgccggcc
     2101 cggttacggc accaacggca ggcgccacgc cggcgtcccg gggtcgagaa accagagccg
     2161 tatcgctgtc agtccgtgca gcgtgagctt tccaaggtgg ggccgctacc gaagccgaac
     2221 aagctggact ggacggacaa ggacggcacc tgattcgccg cgagtggccg gtggcgctac
     2281 cgcgtcaccg aaagcgacaa gggctggttc gccgagcagg ccaccggatc gtggaccccg
     2341 ctgcacagcg acccacagga cgtcgacacc gccaagcgca tcgctgagac ggtcgacggc
     2401 ggggcgcact ggatcgggtg ggccaagtac cgcacccgtg ggcggagatc gaaaagaccg
     2461 cgaaggaagg aggcgatcgc cgggattacc gggcgcgccg agcagatcct cggcgtcgtc
     2521 ggcctcgccg atcgaggcac cgtcgctcac atcaatatcg tgtcatcgcg tccggcttta
     2581 caccgtgata tcggccttcc cactaattcc ggcggcgtaa gttcggctgc gtgccgtcga
     2641 agacacccaa gccacccgtc gatcccgtcc gctggcagcc tccgccagtc gacccgctgc
     2701 cggatttcgg accggctgac ctcaccgtcg tccccgttcc gggcaacgcg ccggaggacg
     2761 tggtggtcga cgcggccgga aatatttgga ccggcgtcga tgacgggcgc atcgtgcgga
     2821 tctcccccga cggtcagccc acagtggtcg gcgaaacagg cggcaggccg ctgggactgg
     2881 ccgttgcccg cgacggccgg ctgctggtgt gcgacagccc acgcggcctg ctggcgatgg
     2941 acaccgacag cggcaagttc gagacactcg ttgaggaggt cgacggccgg cacctgcagt
     3001 tctgctcgaa tgtgaccgaa acacctgacg gcacaatcta tttcaccgag tcgacgagtg
     3061 cattcaccta tccgcacttc aagggcgcga tcatggaggc ccgtccccgc ggcagcctgt
     3121 ttcgtcgcga cgccgacggc acggtgctca ccgttgtgcc cggcctgtac ttcgccaacg
     3181 gcgtcacgca gaccgcggac gggtcggcac tggtgttcgc cgagacgcaa ggccgccgac
     3241 tgtcgaagta ttggttgacc ggcccgcagg cgggagccgt gaccacgctg gtggccaacc
     3301 tgcccggcta tcccgacaac atctccaccg gcgccgacgg ccgcatctgg gtggcacttg
     3361 tctcccccgt caacgccgcc gtggagtggc tggccccgcg cgcgccggca ttgcgcaagc
     3421 tgctgtggaa gctgcccgac cggttgcagc cgcagatcaa gcccgaggtg tgggccgtcg
     3481 cattcgatcc cgacagcggc gaggcgatcg cgggcctgca cagcaagcac ccgtcgttcg
     3541 gcatggtcac cggcctggtc gaaagcgacg gaaagctgtg gatgggctgc atcggcgcgc
     3601 ccgccgtcgc gtattgcgcg atcccgccga gatccgactg atgacacgcg accggctgcg
     3661 gcgcaccaac gacggctact acggcgacgg ctactggctg atcgacaacc gcaccaacgg
     3721 cctggtcctc ggtgagcaga tcgcccgcgg tgtcaacatc ggctaccacc tcgatgccgt
     3781 cgaggagtgg ctgcgcgaca tggcccaagg ccggtcctct cacgtctcgc ctgccggatt
     3841 cgtgtcggtg ccctggtgta acgacattcg gaacgctggc caggtgcggc gtcgtcggcc
     3901 atgcggcacg gatgatggtt cgccccggac aggttgctag agcacgttct tccatcactg
     3961 tgacgtgtcg atcgctagtg tcgaatggta ttgcagccca aatgatttca tggaaaatgg
     4021 aaattgtcca tcattctgcg gaacacgtga ggatgtggcg acgcgcacgg acggcgataa
     4081 caacggcggt gccccgggcg ttggcggttc gggccacgcc ggcaccgcgt gacgcggctc
     4141 ggggaggcac tgtcacgcta aatcgtcacg tgacgaagaa gggtggcccc gggggatatg
     4201 agcccaccca aggggagagg ccttttctaa tcggccggca tgtcatggtc ggcattgtcg
     4261 actcggcggg ttagcggcag acgttcggga gacaagacga gtgctagtcg ccaacgcttg
     4321 ggagttgctg gtcgcgtgcc ggattccgct caaccgctcg tcgctgatcc cgaagtgatg
     4381 cgcgatttcg tggagcacga cgtgtctgac ctcgcggcgt agacgatcgg catcgccggc
     4441 ggcgagtcgc gtgatcggcc cgccgaagat gctgatcgtg tcgggaagca ctccgctgta
     4501 cgtgctgctc cttcgcggca gcggtacgcc gcggtacagg ccgagcagcg gctgccggtc
     4561 gggaggctcg tcctcgacga cgatctcgac gttgctcatc gccgcatgga gatcggccgg
     4621 cagggagctc agcgcgtcgt caatcgcctc ttcgaacgga tcattgagac ccggccccga
     4681 acgcgcatcc atctccgcaa tggtgccaga atcggtgctg ggatagagac ggctccttct
     4741 tgggggcttt gtggcgtcgc ggtaccgtgt cacggtgagc ggccagcagc actggccggc
     4801 ctgggccacc gaacccgtcg atctcctcgc cgccaacccc gcctcaccac gcggtggcca
     4861 tccgctacct gcgaaccatc cgcccgcggc ctccgccgcc acaaaacgcg gcaccggtaa
     4921 cccatcgcgt agaactgctt cacggttcgg gtcggctgca cctgcgtgca caacgcaatt
     4981 ggctgttgcg ctatgtggcg tgccccggga aattctgggc gcccttggcg cccaacagat
     5041 tcggagcgcc tacggcgcat gggaccctga cagaactgcc tttctggcct ccgatggtag
     5101 ccggatgtcc gtctcgcgct aggccaccgg ctgcgctgcg cttggccttc ggctcgaaaa
     5161 cttcccggcc gctcgctgcg ctcgcgggcg gaaacttttc gcccaggacc tagccgctca
     5221 ctcggccatt acccggctcc atctccagtg ccggaaaggc aaaagaacgg gcggccaccc
     5281 ggtcgccccc ttcacccaga aaggtcaacc atgactgaca caaccactac cacgcccgcc
     5341 gccgaggagg ccgtcgcgct cggcgagctg tcccatatcg atccgaacgg gttggaggtc
     5401 ggcgaaaatg tccgtgaata cgccaacctc gacaagtcgt tcctcgacag catc
//
LOCUS       DASWBF010000214         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1069344,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000214 DASWBF010000000
VERSION     DASWBF010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069344"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..589
                     /locus_tag="VF874_07550"
     CDS             95..589
                     /locus_tag="VF874_07550"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX9175939.1"
                     /translation="MSDHLHVTFVCTGNICRSPMAEKMFAHQLRERGLHEHVRVTSAG
                     TGDWHVGSEADTRASRVLRAHGYPTAHCAAQLDDDHLAADLVVALGRNHARMLAHLGV
                     PGERVRLLRSFDPRSGAHALDVEDPYYGAHADFQETFDVIEASLPGLHDWVDEQLAAR
                     GLVD"
     gene            589..1383
                     /locus_tag="VF874_07555"
     CDS             589..1383
                     /locus_tag="VF874_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX9175940.1"
                     /translation="MRRWGFVFRPGWLALALVVAAFAYLCFTVLAPWQLGKNSKTSRE
                     NDQIAASLTSDPVPVTSLLPQADSAQWRRVTATGHYAGATVLARLRVVDGQPAFEVLT
                     PFAVEGGPTVLVNRGYVRPLEASQVPPIPPAPSTPVTITARLRDSEQNADKAPITEAG
                     MPQVYSINTQQVGELTGTPLAAPYLQLVEDQPGGLGVLPLPHLDAGPFLSYGIQWIAF
                     GILAPIGLGYFIYVEIRQRRRESTAKEPAASVTTEEKLADRYGRRR"
     gene            complement(1365..>3592)
                     /locus_tag="VF874_07560"
     CDS             complement(1365..>3592)
                     /locus_tag="VF874_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9175941.1"
                     /translation="LGPRRLRDLPTPIGVFQVHALGLRTDFPALQALDASPGNLRPQT
                     TSFIGRESEVAEIQAAVRAHRLVTLIGVGGVGKTRLATEVAARLADEFPDGVWLFELA
                     AVSDPAAVPDAVAAVLGVTQQPGKRVSDSVAAALEGRVRLLVFDNCEHVLDAAADLVE
                     AILAQSSTVRIVATSREGLGIGDEQLWPVPSLDIGSAAVELFVERARNVAPRFEIADA
                     AEAGAVIEICRRLDGIPLAIELAASRMASMTACEVRDRLDQRFRLLVGSRRGLARHHT
                     LRHAVQWSYDLLGDAEKYLLARCSVFASGFDLESACAVAGSEDDFAALDLLDALVRKS
                     LLVADRSSGRTRFSMLETIRQFAEEQLVAGGEASDARAAHARYFAGRETNVLALWDSP
                     RQREAYDWFTAELANLRTAFRWAADHGDLDVAAPIATYATILGLWVDQYEPIAWAEEL
                     IDPARAVDHPRLATLYVMASQCFISGRPEAAVGYSDVGQMVIGNDCDEVPYGIQGLLG
                     SAYQFIGQPERSVEWYRAQLARGRDTHTLTRACLVMTLMIAGCADEAMAAATGLIEAA
                     EATRNPFAISFALMAYGFAYGDADPVRARDALRRGLVIAQDSGNRDMETHLAACLARL
                     EAEHGDPLAALEYSAVAIGNYHDAGNTVNMRAALAALAACLDRLGCYEPAATIAGFAF
                     DPLTAAWIPEINTAIAQLREVLGDETYESLAREGEAMTTAAMATYAYDEIEQARQRLR
                     P"
BASE COUNT          594 a         1210 c         1218 g          570 t
ORIGIN      
        1 gaatggggtt acggtgcagg ggatttcgaa gatccagaag tgcagcgccc gcttgcccag
       61 gtcccgacgg tgtccgcgct gcgcgaggtg ctcggtgtct gaccacctgc acgtcacgtt
      121 tgtgtgcacc ggcaacatct gccggtcgcc catggcggag aagatgttcg cccatcagct
      181 gcgcgaacga ggcctgcacg aacacgtccg ggtgacgagc gcgggcaccg gtgactggca
      241 cgtcggaagc gaggccgaca cgcgtgcgag tcgcgtactg cgtgcgcacg gctaccccac
      301 cgcgcattgt gcggcgcagc tggacgacga ccacctggcc gcggatctcg ttgtcgcgtt
      361 gggccgcaac cacgcccgaa tgcttgccca cctgggtgtg ccgggcgaac gagtcaggct
      421 gctgcggtcg ttcgacccgc ggtccggcgc gcacgcactc gatgtcgagg acccgtacta
      481 cggcgcgcac gccgatttcc aggagacgtt cgacgtgatc gaggcgtcgc tgcccggcct
      541 gcacgactgg gtcgacgagc agcttgccgc ccgcggtctg gtcgactgat gcggcgctgg
      601 ggcttcgtgt tccggccggg ctggctggcg ctggcgctgg tggtcgcggc gttcgcctac
      661 ctgtgcttta ccgtgttggc gccgtggcag ctcggcaaga acagcaagac gtctcgcgag
      721 aacgaccaga tcgcagcatc attgacgtcc gatccagtgc cggtgacttc gcttctaccg
      781 caggctgatt cggcgcagtg gcgaagggtc accgcgaccg gtcactacgc cggcgcaacg
      841 gttttggcac ggctgcgggt cgtcgacggg cagccggcgt tcgaggtgct gacgccgttc
      901 gccgtagagg gcggaccgac cgtcctcgtc aaccgcggat atgtccgacc gctggaagca
      961 tcgcaggtcc ccccgatccc gcctgcgccg tcgacgccag tgaccatcac ggctcggctg
     1021 cgggattcgg agcagaacgc ggacaaggcg ccgatcaccg aagccggtat gccgcaggtg
     1081 tattcgatca acacccagca ggtcggcgaa ctcaccggaa ctccattggc ggccccttac
     1141 ctgcaactcg tggaggacca gccgggcggt ctgggggtgc tgccgctgcc gcatctcgac
     1201 gcgggcccgt ttctgtcgta cggaatccag tggatcgcgt tcggaatcct cgcgccgatc
     1261 ggcttgggct acttcatcta tgtggagatc aggcagcgcc gccgggagag caccgcgaag
     1321 gagccggcgg cgtcggtgac caccgaggag aagctcgccg accgctacgg ccgcaggcgc
     1381 tgacgggcct gttcgatttc gtcgtaggca tacgtcgcca tcgcggcggt ggtcatcgcc
     1441 tcgccctcgc gggcgagcga ttcgtaggtc tcgtcgccga ggacttcgcg cagctgggcg
     1501 atcgcggtgt tgatctcggg gatccacgct gcggtgaggg gatcgaacgc aaaaccggcg
     1561 atggtggccg ccggctcata acatccgagc cgatccagac acgcggcgag ggcagccaga
     1621 gcggcacgca tattgacggt gttgcccgcg tcgtggtagt tgccgatggc cacagcggag
     1681 tattcgagcg cggccagcgg gtcgccgtgt tcggcctcga gccgggccag gcaggccgcc
     1741 aggtgggtct ccatgtcgcg gttgccgctg tcctgggcga tcaccaggcc ccggcgcaga
     1801 gcgtcacgcg cacggacggg atcggcatcg ccgtaggcga acccgtatgc catcagcgcg
     1861 aacgagatcg caaaggggtt gcgggtggcc tcggcggctt cgatcagacc ggtcgcagcg
     1921 gccatcgcct cgtcagcgca accggcgatc atcaatgtca tgaccaggca ggccctggtg
     1981 agggtatggg tgtcgcgacc gcgtgcgagt tgggcgcggt accactcgac cgaccgttcg
     2041 ggctggccga tgaactggta tgcgctgccg agcaagccct ggatcccgta gggcacctcg
     2101 tcgcagtcat tgccgataac catctggccg acgtcgctgt agccgacagc ggcctcgggc
     2161 cgtccggaaa taaagcactg ggacgccatc acatacaggg ttgcgagccg gggatggtcg
     2221 acggcgcggg cggggtcgat cagctcttcg gcccaggcta tcggctcgta ctggtcgacc
     2281 cacaggccga ggatcgtcgc gtaggttgcg atgggggcgg cgacgtcgag atcgccgtgg
     2341 tcggcggccc agcggaacgc cgtccgcaag ttggccagct cggcggtgaa ccagtcgtag
     2401 gcctcacgtt ggcgggggct gtcccacagg gctaggacgt tggtttcgcg tccggcgaag
     2461 tagcgggcat gggccgcacg cgcatcgctc gcctcaccgc cggcgacgag ttgctcctcg
     2521 gcgaactgtc ggatcgtctc gagcatcgaa aaccgggtcc gccccgagga ccggtcagcg
     2581 accaacagcg acttgcgcac tagggcgtcc agcagatcca aggcggcgaa gtcgtcctcg
     2641 gatcctgcta ctgcacaggc actttcgaga tcaaatccgc tggcgaacac cgaacacctt
     2701 gccagcaaat acttttcggc gtccccgagg agatcgtagg accactgcac cgcgtggcgc
     2761 agcgtgtggt ggcgcgccag tccgcgtcgt gacccgacca gcagccggaa tcgctgatcc
     2821 aggcgatccc gcacctcgca tgccgtcatc gacgccatcc gtgaggccgc cagctcgatc
     2881 gccaacggga tgccgtcgag gcgccggcag atctcgatga ccgcgcccgc ctcggcagcg
     2941 tcggcaatct caaaacgtgg cgccacattg cgggcgcgtt cgacgaacaa ttccaccgcc
     3001 gcggagccga tgtcgagcga cggcaccggc cacaactgct cgtcgccaat tcccaatcct
     3061 tcgcggctcg tcgccacgat ccgcacggtc gacgattgcg cgaggatggc ctcgaccaaa
     3121 tcggccgccg cgtcgaggac gtgctcgcag ttgtcgaaca ccaacaaccg gacccggccc
     3181 tccagcgcgg cggccacact gtcgctcacg cgcttccccg gctgttgggt gacgcccagc
     3241 accgcggcca ccgcgtcggg caccgccgcc ggatcgctga cggcggccag ttcgaacaac
     3301 cagacgccat cggggaattc atcggccagc cgcgccgcaa cctcggtcgc caggcgggtc
     3361 ttgccgaccc cgcccacccc gatcaacgtc accaaccgat gcgcccgcac cgccgcttgg
     3421 atctcggcga cctcagactc gcgcccgata aaactggtgg tctgaggccg caggttcccc
     3481 ggactcgcat ccagtgcctg caacgcagga aagtccgtcc gtaggcccag cgcgtggacc
     3541 tggaaaaccc ctatcggggt gggcaggtcg cgcaaccggc gcggtcccag at
//
LOCUS       DASWBF010000215         8113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_415740,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000215 DASWBF010000000
VERSION     DASWBF010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415740"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..545
                     /locus_tag="VF874_07565"
     CDS             330..545
                     /locus_tag="VF874_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9175942.1"
                     /translation="MTYEAYAVSRTSTRPQVDSARKFGRPAKLNADQAALARRMEENG
                     ETAATNCWTLGIGRTTLYRYLAESGED"
     gene            complement(590..988)
                     /locus_tag="VF874_07570"
     CDS             complement(590..988)
                     /locus_tag="VF874_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dopamine receptor D4"
                     /protein_id="HEX9175943.1"
                     /translation="MCTLGVITAFMMTAPSALADPVDPTPPPPVPDTATQPVSGDPAV
                     TPTGVPHLMSPENLPPGTTTDAAVGRSQVPRLFRLRELWDAIHTEGISLSDALPLLAQ
                     RPLDANAVPPPGVSAGPQPPLPAEPLPPTP"
     gene            1401..1649
                     /locus_tag="VF874_07575"
     CDS             1401..1649
                     /locus_tag="VF874_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9175944.1"
                     /translation="MKADGHTGKDIAKYLGVSRATLYRYLNEEKRRLKRASRSACLAS
                     PARGGTDTSDNRYGRGVLGAVVVGDCWPGPIWPGPIWL"
     gene            complement(1568..1900)
                     /locus_tag="VF874_07580"
     CDS             complement(1568..1900)
                     /locus_tag="VF874_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175945.1"
                     /translation="MTQTPESRTELDPVAGVPHAVEPPPRYDDRYGRPNRLNQALAWV
                     GIVAGVLFVVAVIFFSGFFIGGSLGGHYGGHHRGYYHHYPYSQMGPGQMGPGQQSPTT
                     TAPSTPRP"
     gene            3202..3864
                     /locus_tag="VF874_07585"
     CDS             3202..3864
                     /locus_tag="VF874_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9175946.1"
                     /translation="MQDGAIPRNPATGIRLPKVQGNEPQPLTHTELWQLAEQFDSRRD
                     RVLVVVAGYCGLRWGELAALRWTDIDLDLRRLRVSRAYSEEAPRGEMSPVKDHQARTV
                     PIPRTIVDELTDLRAQSAHSELVFPSANSTPLRNRNFRRDSFDAATAALGLMITPHNL
                     RDTAASLAIQAGASVVAVARLLGHESAATTLNHYAGLFPTDLDDVAIRLDVAAREAIG
                     DQ"
     gene            complement(3967..4042)
                     /locus_tag="VF874_07590"
     tRNA            complement(3967..4042)
                     /locus_tag="VF874_07590"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4007..4009),aa:Arg,seq:cct)
     gene            complement(4078..4896)
                     /locus_tag="VF874_07595"
     CDS             complement(4078..4896)
                     /locus_tag="VF874_07595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9175947.1"
                     /translation="MAKDFRFGIGLLPKDSRSTWQETARRAEGLGYDILQVPDHLGAP
                     APFPALVSAAEVTTIRLGTFVLNAGFYKPALLARDVAALDMLSDGRFELGLGTGYVRE
                     EFEAAELPFPSAGHRVDYLARMTAYMQEHVPNVPILIAGAGDRVLTIAAYHAQIIGIA
                     GGRPGGVAADPLGERVEFVRGVAADRFDDIELNLAVIAVPRDSSGVPNLRLARRYAPD
                     LSDEELLKLPGVLSGTPRDIADTLRRYRDTYGLTYFTVQEHHAESFTKVIAELR"
     gene            complement(4997..5128)
                     /locus_tag="VF874_07600"
     CDS             complement(4997..5128)
                     /locus_tag="VF874_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175948.1"
                     /translation="MRAVPVFTKAVGWVRADYPQVAPGGHVAIVALCCKRPGEPARR"
     gene            complement(5213..5764)
                     /locus_tag="VF874_07605"
     CDS             complement(5213..5764)
                     /locus_tag="VF874_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9175949.1"
                     /translation="MTDKVWKLDASDGQLLVTTGVAGRAARMGHRLTIEMNAWRATVS
                     WSGDDPVAVELDVDVESLEVLRGEGGVTPLSGPEKGLVRSNALKSLDAKRFPAISFST
                     EDVVQNADGYRLTGTLEIHGKSRPQTVDLRVEDLGDSWRMSTDAQVRQSEFGVKPYSL
                     FMGSMKVADEVTVAFTATRSKAD"
     gene            complement(5761..6984)
                     /locus_tag="VF874_07610"
     CDS             complement(5761..6984)
                     /locus_tag="VF874_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9175950.1"
                     /translation="MTYDPRYSPPRPRYPEPRGYQQPYDWRTAPQYHRPSAPAPSAPK
                     RSRGGLLFAGAVVLSVISAAVGGAVGVTMSSPHSRISTMTTANNGTAPTRVQPGSVEE
                     VAAKVVPSVVKLETRLGRATAEGSGIVLDPDGLILTNNHVVSVPGAGPGLSGAETTAT
                     FNDGRIASFTIVGADPMSDIAVIRAKQMSGLTAITIGSSADLRVGQSVVAVGSPLGLE
                     STVTAGIISALNRPVSTVGDADNQNTVLDAIQTDAAINPGNSGGALVNMNGELIGVNS
                     AIATLGDSAGPDAKAGSIGLGFAIPVDQAKRIADQLIATGKASHASLGVRVTSDANTH
                     GARVVEVVNGGPAASAGLPSGAVVTKLDDRVIDSADALVAAVRSKAPGDTVTLTYTDP
                     SGATKTVKVKLGEAS"
     gene            complement(7018..>8113)
                     /locus_tag="VF874_07615"
     CDS             complement(7018..>8113)
                     /locus_tag="VF874_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX9175951.1"
                     /translation="DPTVNEQKAREAGRKAQISTVRNLLGIPIDHFIEVTLVAFFQIA
                     QVVQPITVCLNEVTYDSFSGADFHKGVQQIDAGQAMAFVRQRRDVNDEMFTDLDRTRR
                     QQAFIVSLLSALRHGGSLSSPSDLRQLLDVARQNIAVDSGLDLSSFVQQASALTDRGV
                     SLYTLPITSFSQDSSGEDINTIDVPTIRSIVHNLVAGDLPDTASTTSRTTAPSSSENQ
                     PLSAANGVTLDVVNASKFVGLATRVLTAFSERGFTAGEANTADAIADESTITYGPGAQ
                     AAAEALARQLNLTTSPSTDVERDTVQLTVGMDFASADYLIDTETNTSTPTTTAPATPV
                     ATVSATATGTRTPAPTDLTRMTTDQAPCVK"
BASE COUNT         1324 a         2621 c         2723 g         1445 t
ORIGIN      
        1 cgtcgccgcc gctttcgtcg tcttctcctg cgtgatctcc ggcgccgcgg ccttcctcga
       61 cgtggcgggg cgggtggttg gcttcttcgt cgccgccgct ttcgtcttct tctccttcgc
      121 tgccataccg gtgctcctcc tcgtttcgcg aatcgagtct cgggctcgtc gcgtatacgt
      181 tagccgaaca gcggcacgac accgcctcgc tgcggtcggc ggttctggca gatggttgac
      241 ctcggcgacg tcctcctccg ggcactgtca acttgagcag tccgagggcg attttcgccg
      301 gttcgcctcc gcgattcacc aggtcgcgtg tgacgtatga ggcttatgcg gttagcagaa
      361 cgtccacacg acctcaagtt gacagtgccc gcaagttcgg tcggcccgcc aagctcaacg
      421 ccgaccaagc cgcgctggcc cggcggatgg aggaaaacgg cgagaccgcc gcgaccaact
      481 gttggacgct cgggatcggc cgaaccaccc tgtaccgata tctggccgag agtggcgagg
      541 actgaccaaa ctcccgatgc ctagttcgtc gattcgtgaa ctgagcgcgc tagggtgtcg
      601 gcggcagtgg ctcggcgggc agcggcggct gaggaccggc cgagaccccc ggcggcggca
      661 ccgcgttagc atccagcggg cgctgcgcca acagcgggag tgcgtcagac aagctgatgc
      721 cttcggtgtg gatcgcgtcc caaagctccc gcagacgaaa caggcgcggg acttgcgacc
      781 ggccaaccgc agcatcggtg gtggtcccgg gaggcaggtt ttccgggctc atcaggtgcg
      841 gcaccccggt gggtgtcacc gccgggtcgc ccgagacggg ctgggtggcg gtgtcgggca
      901 ccggcggtgg tggtgtgggg tccaccgggt ccgccagcgc cgacggcgct gtcatcatga
      961 acgccgtgat cacccccagc gtgcacaacc cggctgaccc tctgcgccta accgggggaa
     1021 tggtgacaga gcggcaagcc gtcgttcgga tgccggtctg cgtgcagtcg cggcacaact
     1081 tgtggaatcc cgctatgcac caacggcatc cgcagttgca ccgcactcgc aacctcacct
     1141 cgccgaccaa ttctgtcagc gcggttgagg ctgccgacgg tgaagcccag cttgccgacg
     1201 ggcggtcgcc gcttgcgttc atggctgcgg atcgtagttc cgccatgccg caccgccgag
     1261 gaattcagga gattcactgc gttcttgttg ctgccatcaa ggagcggacc gcgctcaagc
     1321 ggcaggcgtc tgggtacggc acgaagttcg gccgtcccag gaaggtgaag gaccggagcc
     1381 acatcaccac cgcgaggcgg atgaaggccg acggccacac cggcaaggac atcgccaagt
     1441 atctcggggt aagccgggcc acgctgtatc ggtacctcaa cgaggaaaag cgccgcctga
     1501 aacgtgccag ccgtagtgcc tgcttggcgt cgccagcacg gggcggcaca gacacgagcg
     1561 acaaccgtta cgggcgcggt gtgctcgggg ccgttgtcgt cggcgattgc tggcctggcc
     1621 ccatctggcc tggccccatc tggctgtagg ggtagtggtg gtagtaaccg cgatggtggc
     1681 cgccgtagtg gccgcccaag ctcccgccga tgaagaaccc ggagaagaag atcaccgcga
     1741 cgacgaacaa gaccccggcc acgatgccga cccacgccaa cgcctggttg agccggttgg
     1801 ggcggccata tcggtcgtcg taccgtggcg gcggctcaac ggcgtgcggc accccggcca
     1861 cggggtctag ttcagtccgg gattcaggtg tttgggtcat gttcggaatc atgcgcgcgc
     1921 tgaactgctc aattggtggc gtttcggcac cgaatagctg tgaataacct ggcgtcgtcc
     1981 acgtagttgg tgagctggtc cgctgcacca gtggcccgtg cgttgcgagg ccagccgtgg
     2041 cgtaccaccg ctcggcacgt tccggacttc taggctcgtt ggtcatgacg acgctcggtg
     2101 atctgttggg tcggttagac cccgacccag ttcggcgggg caggcacttc gagcgcctct
     2161 gccagtggtt cctcacgaac gatccggtct accagcgcga gcttccctga ccactgtggc
     2221 cacgcctggc tgcaggaggg ggagcaatgc tgctggagcc cgcacatgtg gtttggcgtt
     2281 cacagctccg ggccagcttt gggtcgtccg agtcagtttc atcaaatatg gcaccggcgg
     2341 tggcggaaaa atgttgttgt ggcggatgat ctcgtcatgg catgccaagc cctcgagggt
     2401 cggagccgaa gttgaccgtt accgcgagac gccgccgcgg ggcgctggat ccgagtcgaa
     2461 agccgctctt cgcgagcgag agagtagtta agaagacatc agcactggag acgtgatgaa
     2521 aaagtctatc gggcaatcac attcgatcgg agccgcgcca cggctcctga ccatcaagca
     2581 ggtcgctgac cgcctcacgg tcagtgtcgg gtgcttgcgg gcgtggcgca tccgtggcga
     2641 ggggccgccg gcgattcgag ttggttcggc cttgcgttgg gacgagcgcg aagttgacgc
     2701 ctggttgaac gcgcgtcgcg aatcaggcgg attggcctct tgacgcggat actgcccgcg
     2761 aactaattgg cgagggacag cgcggtgatc gaggtccgga ctggccgtga tggtgctcgc
     2821 gtctatcgag ctcgggttta tcaccgcggt cgatacgtag cgtcacggac tttcgcacgg
     2881 aagcgagatg ctcaggaatg ggaacggcgt caggttgacg gtctacgttc tggagtatgg
     2941 tccgaccccg gtgcggcaga aaggccggtg caggactggt gtgatgcttg gctagccgca
     3001 cagcctcccc gggcgccggc gacggaacgc aagatccgtg aagctatcaa caagcggatc
     3061 gcaaaaacct tcggtcggcg cccggcgata tccgttcgac cgtctgaggt cagggcatgg
     3121 tgcgcagatc tggcacgaac acagtccccg gcgactgcgc gtcactcgct gggcgtctta
     3181 cgccgagtct tcgactacgc agtgcaggac ggggccattc cccggaatcc cgcgacaggt
     3241 atccgcctac ctaaggtcca agggaatgag ccccagccgc tcacacacac tgaactctgg
     3301 cagctcgcag agcaattcga ttccaggaga gaccgagtcc tagtcgttgt tgccgggtac
     3361 tgcggacttc gttggggcga gctggcggcg ttgagatgga ccgatatcga tctcgatctg
     3421 cgtcggctga gagtatctcg cgcgtactcg gaagaggcgc ctcgaggcga aatgtctccg
     3481 gtgaaagatc accaggcacg caccgttccg atcccgagaa ctatcgttga cgaactgacc
     3541 gatctacggg cgcagtcggc ccattccgaa ctcgttttcc cctccgcgaa ctccacgccc
     3601 ctgcgaaacc gcaattttcg acgcgacagc ttcgatgcgg ctacggctgc actgggcctg
     3661 atgatcactc cgcacaacct ccgtgacacc gcagcgtcgc tcgccataca agcgggggcc
     3721 tcggtcgttg cggttgcgcg cttacttggc catgagtcgg ctgcgacgac gctcaatcac
     3781 tatgcagggc tcttcccgac cgaccttgac gacgtagcga tacgcctcga tgtcgccgca
     3841 agagaggcca tcggggacca gtgagcgcca cttcgtgtca ttggctcacc gaccaggcac
     3901 cgaccaggag tcggaccgac tcataaggga ggaagcacct aaagcatcga tgagctgcga
     3961 aaacagtggt gcccccggca ggattcgaac ctgcgacacc ggctttagga gagccgtgct
     4021 ctatcccctg agctacgagg gcctatccgg ctcgcagctt accgggcgcg gtcggcgtca
     4081 gcgcagctcc gcgatcacct tcgtgaagct ctccgcgtgg tgctcctgca cggtgaaata
     4141 ggtcagcccg taggtgtcgc ggtaccgccg cagcgtgtcg gcgatgtcgc gcggcgtccc
     4201 cgacagcacg ccgggcagct tcagcagttc ctcgtcggac aagtccggag catagcggcg
     4261 ggccagccgc aggttcggca cgccgctgga gtcgcgcggg accgcgatga cggcgaggtt
     4321 cagctcgatg tcgtcgaacc gatcggccgc gacgccgcgc acgaactcga cgcgttcacc
     4381 gagcgggtca gccgccacgc cgccgggacg gccgccggcg atgccgatga tctgggcgtg
     4441 gtacgcggcg atcgtcagta cccggtcccc agcgccggcg atcaagatcg gcacgttggg
     4501 gacgtgctcc tgcatgtacg cggtcatgcg ggccaggtag tcgacgcggt gcccggcgct
     4561 cgggaacggc aactcggcgg cctcgaactc ctcgcggaca tacccggtac ccagcccgag
     4621 ctcgaagcgc ccgtcgctca gcatgtcgag cgcggcgacg tcgcgcgcca gcagcgccgg
     4681 tttgtagaag ccggcgttga gcacgaacgt gccgagccgg atcgtcgtca cctcggccgc
     4741 cgacaccaac gccggaaacg gagccggggc gccgaggtgg tcgggaacct gcaggatgtc
     4801 gtagccgagc ccctcggcgc gacgcgcggt ctcctgccac gtcgaccggg agtccttcgg
     4861 caacaacccg atgccgaacc ggaagtcttt ggccatgaaa cgcgatgcta tgcgagcggt
     4921 tgcgccgaat tctgcagcag ggttgtggtt cggctcgatg cacaaccctg atgcagaagt
     4981 cgcggcccga cgctttctag cggcgggctg gctcccccgg gcgcttgcag cacaaggcga
     5041 cgatcgcgac gtggcctcct ggcgccacct gcggatagtc ggcacgcacc cagccgacgg
     5101 ctttcgtgaa cacggggacg gccctcatcg tgctcccctc cttccggtgc ctccctctct
     5161 attcccccac ggtaaggcgg atgaaacctc cgacacaatg gccgcggacc ggtcagtcgg
     5221 ctttggaacg ggtcgcggtg aaagcgacgg tcacctcgtc ggcgaccttc atcgagccca
     5281 tgaaaagcga atacggcttg acgccgaact cggactggcg aacctgcgcg tccgtcgaca
     5341 tccgccacga gtcgcccaga tcctccaccc gtaggtccac cgtctgtggg cgggacttgc
     5401 cgtggatctc cagtgttccg gtcaggcggt agccgtcggc gttctgcacg acgtcctcgg
     5461 tgctgaagga gatggccgga aaccgtttgg cgtcaagcga tttcagtgca ttggaccgga
     5521 caagtccctt ctccggcccg gacagcggcg tgacgccgcc ctcgccgcgc agcacctcca
     5581 gcgactcgac gtccacgtcg agttccacgg ccacgggatc gtcgccggac cagctcaccg
     5641 tggcgcgcca cgcattcatc tcgatggtca gccggtggcc catccgtgcg gcgcggcccg
     5701 cgacgccggt ggtgacgagc agctgcccgt ccgacgcgtc gagcttccac accttgtcgg
     5761 tcacgatgcc tcgcccagct tgaccttgac cgtcttggtt gcccccgacg ggtcggtgta
     5821 ggtgagcgtg acggtgtctc ccggtgcctt cgagcggacc gccgcgacca acgcgtcggc
     5881 gctgtcgatc acccggtcgt cgagcttggt gacgaccgcg ccgctgggca ggcctgccga
     5941 cgctgccggg ccaccgttga ccacctcgac gacgcgggcg ccgtgcgtgt tcgcatcgct
     6001 ggtcacccgc acgcccagcg acgcgtgcga tgccttgccg gtggcgatca gctggtcggc
     6061 gatgcgcttg gcctggtcga ccgggatcgc aaagcccagt ccgatcgatc cggccttggc
     6121 gtcgggaccc gcactgtcgc ccagcgtcgc gatcgctgag ttcacgccga tcagctcgcc
     6181 gttcatgttc accagcgcgc cgcccgagtt gcccgggttg atcgcggcgt ccgtctggat
     6241 ggcgtcgagc acggtgttct ggttgtcggc gtcacccacg gtcgacactg gtcggttcag
     6301 cgcgctgatg atgccggcgg tgaccgtgct ttccagcccg agcggcgacc cgaccgccac
     6361 cacgctttgc ccgacgcgaa ggtcggccga cgagccgatc gtgatcgcgg tcaacccgga
     6421 catttgcttc gcgcggatga ccgcaatgtc actcatcgga tcggcgccga cgatcgtgaa
     6481 cgatgcgatg cgtccgtcgt tgaacgtcgc cgtggtctct gcgccggaca gcccgggtcc
     6541 ggcgccaggc accgacacca catggttgtt cgtcaggatc agaccgtcgg gatcgaggac
     6601 gatgcccgag ccttcggcgg tggcgcggcc gagtctggtc tccaacttca cgacgctggg
     6661 aaccaccttt gccgcgacct cctcgaccga acccggctgc acccgggtag gcgccgttcc
     6721 gttgttcgcg gtcgtcatgg tgctgatgcg cgaatgaggc gacgacatcg ttacccctac
     6781 ggcgccgccg acggcggccg aaatcaccga cagcacaacg gcgcccgcaa acagcagccc
     6841 gccccgagag cgcttgggtg cgctcggcgc gggcgccgac ggacggtggt actgcggagc
     6901 ggtccgccag tcgtagggct gctgataacc gcgtggctcc ggataccgcg ggcgcggcgg
     6961 ggagtaccgc gggtcgtagg tcatgtcgtc agcttccttt tcgcttgccg aacccggtca
     7021 cttcacacac ggcgcctggt cggttgtcat gcgcgtgagg tcagtgggcg caggagttcg
     7081 cgttccggtc gcggtggccg acacggttgc gaccggggtg gccggagctg tcgtggtcgg
     7141 cgtgcttgtg ttcgtctctg tgtctatcag gtaatcggcg ctggcgaagt ccatccccac
     7201 cgtcagttgc accgtgtcgc gctcgacgtc ggtcgacggg ctggtggtga ggttcagttg
     7261 gcgggccagc gcctcggcgg ccgcttgggc gccgggtccg tacgtgattg tgctttcgtc
     7321 ggcgatggcg tctgcggtgt tggcctcgcc ggccgtgaag ccgcgctcgc tgaacgcggt
     7381 cagcacccgc gtggcgaggc ccacgaactt agacgcgttg acgacgtcga gcgtgacacc
     7441 attcgccgcg gagaggggct ggttctcgga tgacgacggc gcagtcgtcc tgctggtggt
     7501 gctcgccgtg tcaggtaggt cgccggcgac gaggttgtgc acgatcgagc ggatggtcgg
     7561 cacgtcgatg gtgttgatgt cctcgccgga ggagtcctgg ctgaacgagg tgatcggcag
     7621 ggtgtagagc gaaaccccgc ggtcagtcag cgcagacgcc tgctggacga agctggacag
     7681 gtccaagccc gaatccaccg cgatgttctg cctcgcgacg tcgagcagtt gacgcaaatc
     7741 gctgggactc gacagcgatc ccccgtgccg cagcgcgctg agcagggaga cgatgaacgc
     7801 ctgctgccgg cgggtgcggt cgaggtcggt gaacatctcg tcgttgacgt cgcggcgttg
     7861 ccgcacgaac gccatcgcct gcccggcgtc gatctgttgg acgcccttgt ggaagtcggc
     7921 acctgagaag ctgtcgtagg tgacttcgtt caggcacacc gtgattggtt gcaccacttg
     7981 ggcgatctga aagaacgcca ccaacgtgac ctcgatgaaa tggtcgatcg ggatgcccaa
     8041 caggttgcgc accgtgctga tctgcgcctt gcgtccggct tcacgcgctt tctgctcgtt
     8101 caccgtcgga tcg
//
LOCUS       DASWBF010000216        10258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1069579,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000216 DASWBF010000000
VERSION     DASWBF010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069579"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1749)
                     /locus_tag="VF874_07620"
     CDS             complement(28..1749)
                     /locus_tag="VF874_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEX9175952.1"
                     /translation="MEQLSFADVDPTAVGAPPACGGLTLAETTFVVVDLETTGGRATD
                     EITEIGAVKVRGGAVLGEFATLVDPGRDIPPQIVQLTGITSAMVSDAPTIDAVLPMFL
                     EFARGAVLVAHNSGFDIGFLKAAARRCGIVWPQPPVLCTVRLARRVLSREEAPSVALA
                     ALAKLFAVATLPTHRALDDARAAVEVLHALIERVGNQGVHTYTDLRAYLPNVTAAQRR
                     KRVLAEGLPHRPGVYLFRGPAAEVLYVGTAVNLRRRVAQYFTGADTRARITEMVTLAA
                     AVDVVECAHALEAGVRELRLLAGHAPPYNRRSRFPHRWWWVVLTDEAFPRFAVVRAPR
                     CARAVGPFRARADAADTAALFARFSGVRTCTTRLARSAVHGPSCPEREVSPCPAARDV
                     SAADYADAPQRAVALIDGRDNSALAAAVAQIEDLAARRRYESAARLRDHAAAAIDVLW
                     RGQRLRALAEVSELVAAAPDGNGGWHIAVIRQAQLAAAVTAPRGVPPMPVIDAACAGA
                     QTVLPADAPLGGALVEEAALIARWLDTSGVRVVRAQPGYAVPVSSAGPWTAWASAARS
                     AKLAGLA"
     gene            1857..2081
                     /locus_tag="VF874_07625"
     CDS             1857..2081
                     /locus_tag="VF874_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175953.1"
                     /translation="MIIDCDDCAVQGAACKDCVVSVLIGTPGSDVAGQAAPTSLREDE
                     RAALEALADAGLAPRLRLVPIHRRNDPGVA"
     gene            2226..3365
                     /gene="ripC"
                     /locus_tag="VF874_07630"
     CDS             2226..3365
                     /gene="ripC"
                     /locus_tag="VF874_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase RipC"
                     /protein_id="HEX9175954.1"
                     /translation="MSSERARWINRRFRRSIIGALAVVSLFGSVLATSVHADPADDAL
                     AKLNELSRQAEQTTEQVHTAQLDLDRKLAAQKAAEARHDADLAAADSAKTQLASYQVT
                     VDKLAAAIYMGGHTDSLSAIMTADSPQQLIDQMSAQDAVANEMAVQMASYRALSAKAA
                     AAEAVSARSATEARVAAEQAAAVRADVQAKQSRLQVQIAVVKSQYMALSPNQRVALGA
                     PPQAPARLPDAPEALPDLPPPDLSINAMPGPEGLMPPNGDVAQPGGAPEAAVVIQAAL
                     SRIGSPYSWGASGPNAFDCSGLVMWSFQQVGISLPHSSQALAAGGQPVDLSQMQPGDV
                     VTYYSDASHVGLYIGDGMMVHASTYGTPVKVAPVGNAPIYNVRRY"
     gene            3388..4131
                     /locus_tag="VF874_07635"
     CDS             3388..4131
                     /locus_tag="VF874_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175955.1"
                     /translation="MAAVGLALIVEVLTAATLLSQPLASPPAAPAHTMRLIDRGGPSE
                     LPGRIAAEIPGAVAAVSAFWGDGWPREIVVVTTGTDADFAAQTGEPSRDWAGVAAVAV
                     ADWVDPERREASGQRIVFAPGANSMSAAALRMVLRHELFHYAARADTALDAPRWLTEG
                     VADFVARPQGSRPGPAAVPARLPSDAELDGPQGSAAYDRAWWFARFVADEYGPPALRR
                     LYMAAGQPQDALGVGWDDLLSRWQRWLAR"
     gene            4143..5270
                     /locus_tag="VF874_07640"
     CDS             4143..5270
                     /locus_tag="VF874_07640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9175956.1"
                     /translation="MSRVLLVTNDFPPRPGGIQAYLEALATHLAETGAHTLTVYAPRW
                     KRADQYDESAPFEVVRHPGTLMLPTPDVDGRMRNLIAARDIETVWFGAAAPLALLARR
                     ARQAGARRVVASTHGHEVGWSMLPVARSALRRIGEDSDVVTFVSRYTRDRFASAFGPA
                     AALEHLPPGVDADRFRPDPAARAELRARYGLGERPVIVCLSRLVPRKGQDMLIRALPA
                     IRSAVDGATLVIVGGGPYLGALRRLAKGAGVDDHVTFTEGVAPAELPAHHAMADVFAM
                     PCRTRGAGLDVEGLGIVYLEASATGIPVVAGRSGGAPETVQQGRTGYVVDGRSTDQIA
                     DAVSRILTDPELAARMGAAGRRWVLDRWRWQTHAARLAELL"
     gene            complement(5267..7066)
                     /locus_tag="VF874_07645"
     CDS             complement(5267..7066)
                     /locus_tag="VF874_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9175957.1"
                     /translation="MRELSLPASSSVAENDNVASSVFEHERTDPDHVIFQRLVDGVWT
                     DVTCAQAAAQIRAAALGLIAEGVNAGDRVAILSATRYEWVILDYAILSVGAVTVPIYE
                     TSSPDQVRWVLEDSGAVLAFTETDAHAQTVKELSDELPALRKVLRIESSGPTALDELS
                     EAGKSVDASELDARLAGLRSDDPATLIYTSGTTGRPKGCQLTHSNLVCEIRGASSCFP
                     TLLRKGGRLLVFLPLAHVLARALSMTAFANKVTLGYTGDIKNLVPMLGEFKPTVVVSV
                     PRVFEKVYNTAELNARDSGKGKIFELAARTAIEWSEAQETGGPGVLLRALHALFDRLV
                     YSKLRAALGGNCTASISGGAPLGARLGHFYRGIGVSIYEGYGLTETSAAITVNRIGDL
                     KVGTVGKLLPGNSMAVADDGELLVRGGVVFGGYWRNEKATNEAIDTDGWFHTGDLGSF
                     DEQGFLSIVGRKKEIIVTAGGKNVAPAVLEDQLRAHPLISQAMAVGDAKPFIGALIAI
                     DPEAFDGWKQHHGKDAAASVEDLADDPDLVAEIDVAVKDANQAVSKAEAIRKFAILPV
                     DFTVVTGELTPTLKVKRKVVADKFADEIESLYR"
     gene            7116..7505
                     /locus_tag="VF874_07650"
     CDS             7116..7505
                     /locus_tag="VF874_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase / dehydrase and lipid
                     transport"
                     /protein_id="HEX9175958.1"
                     /translation="MHSIQVADQTFVAADPADVGTAVSDAGSWRRWWPDLHLEVVEDR
                     AEKGIRWTVTGALTGTMEVWLEPMLDGVNLHYFLHAEPAGAAAWQLARMNVAKMTHQR
                     RVAGKNMAFEVKGRLEASRQVGASPLP"
     gene            7524..7958
                     /locus_tag="VF874_07655"
     CDS             7524..7958
                     /locus_tag="VF874_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9175959.1"
                     /translation="MADSTSQTIYIDADPKTVMDVIADIGSYPQWVSEYKETEVLEAD
                     AEGYPKTARLLLDAAVLKDTMVLSYDWPPDHKSVRWHLVSSSLLRSLNGAYHLAPKGS
                     GTDVTYELSVDLIIPMIGLLKRKAERRLTDTALKDLKKRVEG"
     gene            7955..9214
                     /locus_tag="VF874_07660"
     CDS             7955..9214
                     /locus_tag="VF874_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX9175960.1"
                     /translation="MTESAPPAQRISLFVGKGGVGKSTLAAATAVSDARAGFRVLLMS
                     TDQAHSVGDVLGVPVTPTGERRVTRVLEDDVDAGGGLLDALALDTLALLEARWHEAAG
                     PLGKRFGDSDLGDVAPEELSALPGIQEVLGLHEVGELAASGEWDHVVVDCASTADALR
                     MLTLPATFGMYLERAWPRHRRLARAAEEPGSAALVGMLERMSAGMEHVSALLTDESRV
                     GAHLVMTAERVVAAEAARTLGALSLMGVRVDELIVNQVLVKDDSYEYRNLPDHPAFDW
                     YAERIAEQQAVLDELAGRIGDVELVLAPHLASEPIGPKALLELLDTVRRLDGSPPPGP
                     IAPVVDRESGSGLEAVYRLRLELPQVDPGSLTLGRVDDDLIIGSSGMRRRVRLASVLR
                     RCVVTNAQLRGSELTVRFRPNPEVWPQ"
     gene            9211..9573
                     /locus_tag="VF874_07665"
     CDS             9211..9573
                     /locus_tag="VF874_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175961.1"
                     /translation="MTGAHTDLTPELRALASAILDRLDPAIRAAAAVATGRGDSPGKC
                     QQVWCPVCAVAALVQGEQHPMLDVVAEHSVALLTVVRALVDADGASPPDGDGPGEPAP
                     PPPGRYQHIPITVEDSQE"
     gene            9614..>10258
                     /locus_tag="VF874_07670"
     CDS             9614..>10258
                     /locus_tag="VF874_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9175962.1"
                     /translation="MWYWLFKYIFMGPLLSLMARPKTEGLEHIPDSGPAILASNHLAV
                     ADSFIKPLVIRRRITFLAKAEYFTGKGLKGWFMRWFYTVAGQVPIDRSNADAAQAALD
                     TAKRILGEGKLLGMYPEGTRSPDGRLYKGKTGLARLVLETGAPVIPIALIGTDALNPP
                     GTKMWRFAKVTVRVGKPLDFSRFEGLAGNRFIERAVIDEVMYELMQLSGQEYVDI"
BASE COUNT         1549 a         3499 c         3673 g         1537 t
ORIGIN      
        1 gtccaacttg caggacgcaa cacggattca ggctaaaccc gcgagcttcg ctgaccgggc
       61 cgccgacgcc cacgccgtcc acggtcctgc cgaactgacg ggtaccgcat agcccggctg
      121 cgcgcgcacg acccggacgc ccgacgtgtc cagccaacgg gcgatcagcg cggcctcttc
      181 gaccagcgcg ccgcccagcg gagcgtcggc gggaagcacg gtctgcgcgc cggcgcacgc
      241 ggcgtcgatc accggcatcg gcggcacacc gcgtggcgcc gtgacggccg cggccagctg
      301 cgcctgccgg atcacggcga tgtgccagcc gccgttgccg tcgggtgcgg ccgcgaccag
      361 ctcgctgacc tcggccaacg cgcgcaaccg ctggccacgc cacaacacgt cgattgcggc
      421 agcggcgtgg tcccgcagtc gggcggccga ctcgtagcga cggcgcgccg ccaggtcctc
      481 gatctgcgcg accgccgcag cgagtgcgga gttgtcgcgg ccgtcgatca gcgcgaccgc
      541 gcgctgtggc gcgtcggcgt agtcggccgc cgagacgtcc ctggccgccg ggcacggcga
      601 cacttcccgc tccgggcacg acgggccgtg caccgcggag cgggccaacc gcgtggtgca
      661 ggtgcggacc ccgctgaagc gagcgaacag cgcggcggtg tcggcggcgt cggcacgggc
      721 gcggaacggc ccgaccgcgc gggcgcaccg cggcgcgcgg accaccgcga accgcgggaa
      781 cgcctcgtcg gtgagcacca cccaccacca gcggtgcggg aaccgcgacc gccggttgta
      841 gggcggcgcg tggccggcca gaagtcgcag ctcacgcaca ccggcctcca gcgcatgcgc
      901 gcactcgacg acatcgaccg cggcggccag cgtcaccatc tcggtgatcc gggcacgggt
      961 gtcggcgccc gtgaagtact gggcgacgcg gcggcgcagg ttgaccgccg tcccgacgta
     1021 gagcacctcg gcggccgggc cgcggaacag gtagacgccc ggccggtgcg gcaggccctc
     1081 ggcgagcacc cgcttacgcc gttgcgccgc agtgacattc ggcagatacg cgcgcaggtc
     1141 ggtgtaggtg tgcacgcctt ggttgccgac ccgctcgatt agcgcgtgca acacctcgac
     1201 ggcggcccga gcgtcgtcga gcgcgcggtg agtgggcagg gtcgccaccg cgaacagctt
     1261 ggccagtgcg gcaagggcga cactgggcgc ctcctcgcgg gacagcaccc ggcgggccag
     1321 ccgcaccgtg cacaacaccg gcggctgggg ccagacgatg ccgcaccggc gcgccgccgc
     1381 cttgaggaag ccgatgtcaa agccggagtt gtgggcgacc agcaccgcgc cgcgggcgaa
     1441 ctccaggaac atcggcagca ccgcgtcgat ggtcggtgcg tccgaaacca tcgctgacgt
     1501 gatgcccgtg agctgcacga tctgcggcgg gatgtcgcga ccggggtcga ccagcgtcgc
     1561 gaactcgccc agcaccgcac cgccgcgaac cttgaccgcg ccgatctcgg tgatctcgtc
     1621 ggtggctcgt ccgccggtgg tctccagatc gaccacaacg aacgtggttt cggcgagcgt
     1681 caatcccccg caagcgggag gtgcccccac agccgtcggg tccacgtcgg cgaagctgag
     1741 ctgctccatg tgaggcgacg ctaggcagtg gtaccgacag gagcggggat ttggggggac
     1801 ttgtcggggg gtgtcgctaa cgtcgcgggc aggacgacgg agaggacggt caacgggtga
     1861 tcatcgactg cgacgactgc gcggtacagg gcgcagcctg taaggactgt gtggtcagtg
     1921 tgctgatcgg cacgccgggg tccgatgtcg ccggccaggc ggctccgacc tctctgcgcg
     1981 aggacgaacg cgcggcgctc gaagcgcttg ccgacgcggg cctggcgccg cggctgcgcc
     2041 tggttccgat ccaccggcgc aacgatcccg gtgtcgcgtg acaattcgcc cgaaagatgc
     2101 aacgtctaag attgcgtctt attaatgccg tcctgttgga caagctcgat gccgtttcgt
     2161 aacctatctg agacctaagc aggcgagggc ctttggccac ggcacctaat aaggattctc
     2221 caaccttgag ttccgaacgt gcgcggtgga ttaatcgccg tttccggcga tcgattatcg
     2281 gtgcgttagc agtcgtttcg ctgttcggat ccgttctcgc caccagcgtg cacgcggacc
     2341 cggccgacga cgcgctggcc aaactcaacg agttgtcccg tcaggccgag cagaccaccg
     2401 aacaggtgca tacggcgcag ctcgatctcg ataggaagct cgccgcgcag aaggccgccg
     2461 aggcgaggca cgacgccgac ctggccgccg ccgactccgc caagacgcaa ctggcgtcgt
     2521 accaggtcac cgtcgacaag ctcgccgccg ccatctacat gggcgggcac accgacagcc
     2581 tgagcgcgat catgaccgcg gactccccgc agcagctgat cgaccagatg tcggcgcaag
     2641 atgccgtcgc caacgagatg gcagtgcaga tggccagtta ccgcgcactg agcgcgaagg
     2701 ccgctgcggc cgaggcagtg tcggcgaggt cggctaccga ggcccgcgtc gccgccgagc
     2761 aggccgccgc ggtgcgtgcc gacgtgcagg ccaagcagag caggctgcag gtccagatcg
     2821 cggtggtcaa gtcgcagtac atggcgctca gcccgaacca gcgggtggcg ctgggagctc
     2881 ctccgcaggc gccggccagg ctgcccgacg cgcctgaagc gctgcccgac ctgccgccgc
     2941 cggacctctc catcaacgcg atgcccgggc ccgaagggct aatgccgccg aatggcgacg
     3001 tcgcgcagcc gggcggagcg cccgaagccg cggtcgtgat ccaggctgcg ctgagccgga
     3061 tcggctcgcc gtactcgtgg ggggcctctg gtccgaacgc cttcgactgc tccggtctgg
     3121 tgatgtggtc gttccagcag gtcggcatct cgctgccgca ctccagccag gcgctggctg
     3181 cgggcggcca gccggtggac ctgtcgcaga tgcagcccgg cgacgtggtc acgtactact
     3241 ccgacgcgtc ccacgtcggg ctctacatcg gcgacggaat gatggtgcac gcgtccacct
     3301 acggcacacc ggttaaggtg gcgccggtgg gcaatgctcc gatctacaac gtccggcgct
     3361 actgagcatc cgctgcggcg gctcttggtg gccgctgtcg gcttggcgtt gatcgtcgag
     3421 gtcctcaccg ccgcaacgct gctgagccag cccttagcgt ctcctcctgc cgcccccgcg
     3481 cacacgatgc ggctcatcga ccggggcggg ccctctgaac tgcccgggcg gatcgccgcg
     3541 gagatccctg gggccgtcgc cgcggtgtcg gcgttctggg gggacggctg gccgcgcgag
     3601 atcgtcgttg tcaccaccgg caccgacgcc gacttcgccg cccagacagg tgaaccgagc
     3661 cgcgactggg ccggcgtcgc ggcggtggcc gtggccgatt gggtggatcc cgagcgtcgg
     3721 gaggcgtcgg gtcagcggat cgtcttcgcg ccgggcgcga actcgatgag cgcggcggct
     3781 ttgcgaatgg tgttgcggca tgagcttttt cattatgctg cccgtgctga caccgctctc
     3841 gacgcgccgc gctggctgac cgagggcgtc gcagacttcg tcgcacgtcc gcaagggtcg
     3901 cgtcccgggc cggcagccgt gcccgcacgg ctgccgtcgg acgccgagct cgacggcccg
     3961 caggggtcgg cggcctatga ccgggcgtgg tggttcgcga ggttcgtcgc cgacgagtac
     4021 gggccgcccg cgctgcggcg gctctacatg gcggcggggc aaccgcagga cgccctgggt
     4081 gtcggatggg acgacctgct ctcgcgctgg cagcgctggc tcgctcggta gattagtcag
     4141 ccgtgtcgcg ggtcctgctg gtcaccaacg acttcccccc gcgtcccggc ggcatccagg
     4201 cgtatctgga ggcgctggcc acgcacctgg ccgagacggg agcgcacacg ctgacggtgt
     4261 acgcgccgcg ttggaagcgc gccgaccagt acgacgagag cgcgccgttc gaggtggtgc
     4321 gccatccggg caccctgatg ctgcccaccc cggacgtgga cgggcggatg cgcaacctga
     4381 tcgcggcgcg ggacatcgag accgtgtggt tcggggcggc cgcgccgctg gcgctgctgg
     4441 ctcggcgcgc gcggcaggcg ggtgcacggc gcgtggtggc cagcacgcac gggcacgagg
     4501 tgggctggtc gatgctgccg gtggcgcgct cggcgctgcg ccggatcggc gaggacagcg
     4561 acgtggtgac gttcgtcagc cgctacaccc gcgaccgctt cgcgtccgcg ttcggcccgg
     4621 ccgcagcgct cgagcacctg cctcccggcg tcgacgccga ccggttccgt cccgaccccg
     4681 cggcccgcgc cgaactgcgc gcccgctacg ggctcggtga gcggccggtg atcgtgtgcc
     4741 tgtcgcggct ggtgccgcgc aagggccagg acatgctgat ccgtgcgctg ccggcgatcc
     4801 ggtcggccgt cgacggcgcc acgctggtga tcgtcggcgg cgggccgtat ctgggcgcgc
     4861 tgcgccggct ggcgaagggc gcgggtgtcg acgaccacgt gacgttcacc gagggcgtgg
     4921 cccccgcgga gctgcccgcc caccacgcga tggccgacgt gttcgcgatg ccgtgccgca
     4981 cccgcggggc cgggctcgac gtcgaggggc tgggcatcgt ctacctggag gcctcggcga
     5041 ccgggatccc cgtggtagcc ggtcgttccg ggggagcgcc ggaaaccgtg cagcagggcc
     5101 ggaccggcta cgtcgtcgac ggccggtcga ctgatcagat cgccgacgcg gtcagccgca
     5161 tcctcaccga cccggagctg gcggcccgga tgggcgcggc cggccggcgc tgggtgctcg
     5221 accgctggcg ctggcagact cacgccgcgc ggctggccga gctgctctag cggtacaggg
     5281 actcgatctc gtcggcgaac ttgtcggcga cgaccttgcg cttgaccttc agggtcggcg
     5341 tcagttcgcc ggtgacgacg gtgaagtcga ccggcaggat cgcgaacttg cggatcgcct
     5401 cggccttgct caccgcctgg ttggcgtcct tgaccgcgac gtcgatctcg gcgaccaggt
     5461 cggggtcgtc ggcgagatcc tcgaccgacg cggcggcgtc cttgccgtgg tgctgcttcc
     5521 acccgtcgaa cgcctcgggg tcgatcgcga tcagcgcgcc gatgaacggc ttcgcgtcgc
     5581 cgaccgccat cgcctggctg atcagtgggt gagcccgcag ctggtcctcg agcacggcag
     5641 gtgcgacgtt cttgccgccc gcggtcacga tgatctcctt cttgcgcccg acgatcgaca
     5701 ggaacccctg ctcgtcgaag ctgcccaagt cgccggtgtg gaaccagccg tcggtgtcga
     5761 tggcctcgtt ggtggctttc tcgttgcgcc agtagccgcc gaacaccacc ccgcccctga
     5821 ccagaagctc gccgtcgtcg gcgacggcca tgctgttgcc gggcaacagt ttgccgacgg
     5881 tgccgacctt caggtcgccg atccggttca cggtgatcgc agcgctggtc tcggtcagcc
     5941 cgtaaccctc gtagatcgac accccgatgc cacggtagaa gtggccgagc cgggcgccca
     6001 gcggtgcgcc gccggagatc gacgcggtgc agttgccgcc gagcgcggcg cgcagcttgg
     6061 agtacaccag acggtcgaac agcgcgtgca gggcccgcag cagcacgccc gggccgccgg
     6121 tttcctgggc ctcgctccac tcgatcgccg tgcgcgcggc gagctcgaag atcttgccct
     6181 tgccggagtc ccgggcgttc agttcggcgg tgttgtagac cttttcgaac acccgcggca
     6241 ccgacaccac gaccgtcggc ttgaactcgc cgagcatcgg caccaggttc ttgatgtcgc
     6301 cggtgtagcc gagggtcacc ttgttcgcga acgctgtcat cgacagcgcg cgcgccagca
     6361 cgtgggccag cggcaggaac accagcagcc gtccgccctt gcgcagcagt gtggggaagc
     6421 agctcgacgc gccgcggatc tcgcacacca ggttggaatg cgtcaactgg cagcccttcg
     6481 gccggccggt ggtgcccgag gtgtagatca gcgtggccgg gtcgtcggac cgcaggccgg
     6541 ccagccgagc gtcgagttcg cttgcgtcca ccgactttcc ggcctcggac agctcgtcga
     6601 gcgccgtcgg gccggacgac tcgatccgca gcaccttgcg caacgcgggc agctcgtctg
     6661 acagctcctt gacggtctgt gcgtgcgcgt cggtctcggt gaaggccagc accgcaccgg
     6721 aatcctccag cacccagcgc acctggtcgg gtgagctggt ttcgtagatc ggcaccgtca
     6781 ccgcgccgac cgacaggatc gcgtaatcga ggatcaccca ctcgtagcgg gtcgccgaca
     6841 ggatggccac ccggtcgccg gcgttgacgc cctcggcgat caaacccagc gctgcggcac
     6901 ggatctgggc cgcggcctgc gcgcacgtta cgtccgtcca cacgccgtcg acgagacgct
     6961 ggaagatcac gtgatccggg tcggtgcgtt catgctcgaa aaccgagctg gcgacgttat
     7021 cgttctccgc gacggaagac gacgcaggaa gactcaattc acgcatgatc accagcctag
     7081 cgacgctcga aaaggccgtg ggaagctgtt aggccatgca cagcatccag gtcgccgacc
     7141 agacgttcgt cgccgcggac ccggccgacg tcggcacagc ggtctccgac gcgggcagct
     7201 ggcgccggtg gtggccggat ctgcacctgg aagtggtcga ggaccgcgcc gagaagggga
     7261 tccggtggac ggtcaccggc gcgctgaccg gaacgatgga ggtttggctg gagccgatgc
     7321 tcgacggggt gaacctgcac tacttcctgc acgccgaacc cgccggcgcc gcggcatggc
     7381 agctcgcgcg gatgaacgtg gcgaagatga cccatcagcg ccgggtggcg ggcaagaaca
     7441 tggccttcga ggtcaagggc aggctggagg cttcccggca ggtgggcgcg tcgccgttgc
     7501 cctgacttgg gtaccgtttc aacgtggcag attcgacctc gcagacgatc tacatcgacg
     7561 cggacccgaa gacggtgatg gacgtgattg ccgacatcgg ctcctacccg cagtgggtgt
     7621 cggagtacaa ggaaacagag gtactcgaag ccgacgccga gggctatccg aaaaccgcgc
     7681 ggctgctgct cgacgcggcc gtgctcaagg acacgatggt gctgtcctac gactggccgc
     7741 cggaccacaa gtcggtgcgc tggcacttgg tgtccagttc gctgctgcgt tcgttgaacg
     7801 gcgcatacca tctggcgccc aagggatctg ggacggacgt cacctacgag ctgtcggtgg
     7861 acctgatcat tccgatgatc ggactgctca aacgcaaggc cgagcgaagg ttgaccgaca
     7921 ccgcgttgaa ggacctcaag aaacgagtcg agggctgacc gagtcggcac cgcccgccca
     7981 gcggatcagt ctctttgtcg gcaagggcgg agtaggcaag tcgacgctcg ccgcggccac
     8041 cgcggtcagt gatgcgcgcg cgggcttccg ggttctcctg atgtccaccg accaggcgca
     8101 ctcggtgggc gacgtgctcg gcgtaccggt gactccgacc ggcgaacgcc gggtcacgcg
     8161 ggtgctcgag gacgacgtgg acgccggcgg cggcctgctc gacgcgctgg cgctcgacac
     8221 tcttgcgctg ctggaggcac ggtggcacga ggcggcgggc ccgctcggga agcggttcgg
     8281 ggactcggac ctgggtgacg tcgcgccgga ggaactgtcg gcgctgcccg gcatccagga
     8341 ggtccttggc ctgcacgagg tcggcgagct cgcggcgtcg ggtgagtggg atcacgtggt
     8401 cgtcgactgc gcgtcgaccg ccgacgcgct gcggatgctg acgctgcccg cgacgttcgg
     8461 gatgtatctg gagcgcgcgt ggccccggca ccgccggctg gcccgcgcgg ccgaggagcc
     8521 gggctcggcc gcgctggtgg gcatgctgga gcggatgtcc gccgggatgg aacacgtcag
     8581 cgcgctgctc accgatgagt cgcgggtggg cgcacatctg gtcatgacgg ccgagcgcgt
     8641 ggtcgccgcg gaggcggccc gcacgctggg cgcgctgtcg ctgatgggcg tgcgcgtcga
     8701 cgagctgatc gtgaatcagg ttctggtcaa ggatgattcg tacgagtacc gcaatcttcc
     8761 cgaccacccc gccttcgact ggtacgccga acgcatcgcc gagcagcagg ccgtgctcga
     8821 cgaactcgcg ggccggatcg gtgacgtgga gctggtgctg gccccgcatc tggccagcga
     8881 gccgatcggc ccgaaggcgc tcctggagct gctcgacacg gtgcgccgac tcgacggatc
     8941 gccgccgccg gggccgatcg cgccggtcgt cgatcgcgag tcggggtcgg gcctcgaagc
     9001 cgtgtaccgg ttgcggctag agttgccgca ggtcgacccg ggctcgctga cgctgggccg
     9061 ggtcgacgac gacctgatca tcggatcctc ggggatgagg cgccgggttc ggctggcttc
     9121 tgtgctgcgg cgatgcgtcg tcaccaacgc gcagctgcgg ggaagtgaac tgaccgtgcg
     9181 atttcgaccg aatccggagg tgtggccgca gtgacaggcg ctcacaccga cctaacaccg
     9241 gagctgcggg cgctggcttc ggcgatcctt gaccggctcg acccggcgat acgcgctgcg
     9301 gcggcggtcg cgacgggccg cggcgactcg ccggggaagt gccagcaggt gtggtgcccg
     9361 gtgtgcgcag tggccgcgct cgtccagggc gagcagcacc cgatgctgga cgtcgtcgcg
     9421 gagcacagcg tcgcgctgct gacggtcgtg cgggcgctgg tcgacgccga cggcgcgtcg
     9481 ccgcccgacg gggacggtcc cggcgaaccc gcgccgcctc cgcccggccg ctatcagcac
     9541 atcccgatca cggtcgaaga ctcgcaagag taggtagagt tagcgaaaac tgcgccccga
     9601 gccaggaggg tccatgtggt attggctgtt caagtacatc ttcatgggac ccttgctctc
     9661 tctgatggcc aggccgaaaa ctgaagggct tgaacatatt ccggactccg gaccggcgat
     9721 actcgcgagc aaccacctgg ccgtcgccga cagcttcatc aaaccgctcg tgatcaggcg
     9781 tcgcatcacg ttcctggcca aggcggagta cttcaccggc aagggcctga agggctggtt
     9841 catgcgctgg ttctacaccg tggcgggtca ggtgcccatc gaccgcagca acgccgacgc
     9901 cgcccaggca gcgctggaca ccgcgaagcg aatcctcggc gaaggaaagt tgctcggcat
     9961 gtatccggag ggcacgcgct cgccggacgg gcgcctgtac aagggcaaga ccggactggc
    10021 tcgactggtg ctcgaaaccg gcgcaccggt cattcccatc gcgctgatcg gcaccgacgc
    10081 tctcaacccg ccgggcacca agatgtggcg gttcgccaag gtgaccgtgc gggtcggcaa
    10141 gccgctggac ttctcccgat tcgaggggct ggccggcaac cgcttcatcg agcgcgcggt
    10201 gatcgacgag gtgatgtacg agctgatgca gctgtccggc caggagtacg tcgacatc
//
LOCUS       DASWBF010000217         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_220904,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000217 DASWBF010000000
VERSION     DASWBF010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220904"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VF874_07675"
     CDS             <1..178
                     /locus_tag="VF874_07675"
                     /inference="COORDINATES: protein motif:HMM:NF023257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HEX9175963.1"
                     /translation="STFQSVIDWLRAGYPDEGPRTGHSPLLALNGPIALSPRQTEQIV
                     AAAVVLSTLQRGNY"
     gene            complement(555..953)
                     /locus_tag="VF874_07680"
     CDS             complement(555..953)
                     /locus_tag="VF874_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9175964.1"
                     /translation="MPLLTGVHHVSVPTSDPLAGSDWYVRVFGFATLLIEERESDVVA
                     VLLQHPCGARLLLRHAAAPLVALRGYTLFGLSVATHTELLRWVEHLTTFDVEHSAVHE
                     AHLGWAVTVTGPDLVRIQLHTDEGPSGEDD"
     gene            complement(1274..1570)
                     /locus_tag="VF874_07685"
     CDS             complement(1274..1570)
                     /locus_tag="VF874_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175965.1"
                     /translation="MAALAATTLIAPGIVLGRPVTTLHAATAAVVVGAGVAAHVLSLD
                     LVGIVLACRRRLAADMADHGATPAGRAAPDMGAGGRHAPRSAGAVRDGVTPSRS"
     gene            complement(1613..1756)
                     /locus_tag="VF874_07690"
     CDS             complement(1613..1756)
                     /locus_tag="VF874_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175966.1"
                     /translation="MVNDPPSRSAAVRASPRILVRGDWSTARWIGALSLLCVSGWVLV
                     LAA"
     gene            complement(1762..2904)
                     /locus_tag="VF874_07695"
     CDS             complement(1762..2904)
                     /locus_tag="VF874_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9175967.1"
                     /translation="MREDIPLGRVFGCPVKVNWTVLVILWLFTWSLAATLPHTAPGYP
                     AAIYWLAGVGGAAVLLASLLAHELSHAVVARRAGVEVSSVTLWLLGGVARLNSDAKTP
                     GAEFRIAIAGPAMSVGLAAVFGAAAAIAQAAGLPAIVAAVAAWLAAINLLLGVFNMLP
                     GAPLDGGRVLAAYLWRRTGDAERAAVGAARAGRILAFIFVGLGLAQVLAGAMVPGLWL
                     AFIGVFVHSAARDEERRLRSRQLLTGVRVADAMTPHPRTAPAAVTAENFIQHYLLGGP
                     HSAYPVVAPDGSVLGMVTLTQLRRVAPDHRATTLLRDIAVPLHELATAAPGEALTALL
                     GRLSATPGGRALVIDGGHVVGIVTAADIAKLLDARDLALSSNRDAP"
BASE COUNT          671 a         1336 c         1301 g          617 t
ORIGIN      
        1 cagcacattc caatcggtca tcgactggct ccgcgccggc taccccgatg aagggccccg
       61 caccggacac tcgccgctac ttgccctcaa cggacccatc gcgctgtccc cgcgacaaac
      121 cgaacagatc gtcgccgccg cggtcgtact atcaaccctg caacggggaa actactgaga
      181 aaacctcagc gtcgatagcg catccaccgg gagtgtgtcc ctagagctcc tctgtcaacc
      241 ccgcgttcgt gagtaggcct gattgaccgg ccagcatggc tcgatggacc acatcggaga
      301 gtcgatgttt gaggatgcgg agtgcttcca cgcccgccgt caccgctgtt cttgcggcgg
      361 tcgagcagtc gccgcgcggg cggtgggcgg tgtagaaacg cgctttgggt aagtgcgatc
      421 cggcgcatgg ctgcagttgt ccattcccct tgcgggacaa acgatgtcac tgcctatcat
      481 tggttccgat aacgtgccga tcggtagcgt tgacttatgt tggagatgga tcggcgcaca
      541 cggcacgcaa gtgcctagtc gtcttcgccg ctgggtccct cgtccgtgtg gagctggata
      601 cggactaggt ccgggccggt gacagtcacc gcccagccca gatgcgcctc gtgcacagcg
      661 ctgtgttcga catcaaacgt tgtgaggtgt tccacccagc gcagcagctc ggtgtgtgtc
      721 gcgacactca gaccgaacaa cgtgtacccc cgcaatgcga ccaggggcgc ggcggcgtgc
      781 cgcaacagca gacgggcacc gcacggatgt tgcagcagca cggccactac gtcgctttcg
      841 cgttcctcga tcaacaaggt ggcgaaaccg aatacccgta cataccagtc gctgccggcc
      901 agcggatcgc tggtcggcac actcacgtgg tgtacgccag ttaacagggg cattcgatca
      961 ttcttaccgc cgcgcccacc gtcgtcgccg cagggtgcgg tcgccgaacc gccgggaagg
     1021 gtgagcgctt tcttctccgg tgcgcgccca gatggcatct gcttaccggc gcctcagcgg
     1081 cgtcgaccga ccccgatcag gcagctcgag tcgttcgacg cactgcacat gtccgcgtgt
     1141 acgggttagg gaaaatggcg ttttcagacg gtgcgtcgac gctgtattga taacaacaga
     1201 cccctcgatc acgtttctac cagtgatggc tccggtaagt cagccgccgc agctgcccgc
     1261 gtagggtgaa tcttcacgaa cgtgatggag tgactccatc acgaacggcg ccagcggatc
     1321 tcggtgcgtg gcgtccacca gcacccatat ccggggcagc gcgtccggcc ggggtcgcgc
     1381 cgtggtcggc catatcagca gccagcctgc ggcggcaagc aagcacgatg ccgaccagat
     1441 caagcgacaa cacatgagcc gccacgccgg cgccgaccac gaccgccgcg gtggcggcgt
     1501 gcagcgttgt caccggccgg cccaggacga tgccgggcgc gatcaacgtc gtcgccgcca
     1561 acgcggccac cgagaagccg agcccgccct gccaatcggc cgggcggtgc tgctaggccg
     1621 ccagcacgag cacccagccc gacacgcaca gcaggctcag cgcgccgatc cagcgcgccg
     1681 tcgaccagtc gcccctgacg aggatgcgcg gactcgcccg taccgcggcg ctgcggctcg
     1741 gcgggtcatt gaccatcgcg gtcacggcgc gtcgcggttc gacgagaggg cgaggtcgcg
     1801 cgcgtcgagc agtttggcga tgtcggccgc ggtgacgata cctacgacgt gcccgccgtc
     1861 gatgaccagt gcgcgaccgc cgggtgttgc ggaaagccgg cccagcagcg ccgtcaacgc
     1921 ctcgccgggc gccgcggtgg cgagctcgtg cagcgggacg gcgatgtcgc gcaacagagt
     1981 tgtggcgcgg tggtcgggcg cgaccctgcg cagctgtgtc agcgtgacca tgcccaggac
     2041 agatccgtcg ggcgccacga ccgggtaggc ggagtgcggg ccgcccagca ggtagtgctg
     2101 gatgaagttc tccgcggtga ccgccgcggg agcggtgcgc ggatgaggtg tcatcgcatc
     2161 ggcgacgcgc acaccggtga gcagctgccg tgaccgcagt cgcctttcct cgtcgcgggc
     2221 ggcgctgtgc acgaacacac cgatgaacgc cagccacagg cccggcacca tcgcgccggc
     2281 cagaacctgc gccaacccga gcccgacgaa gatgaacgcg aggatgcggc cggcccgcgc
     2341 ggcacccacg gccgcgcgct cggcgtcacc ggttcggcgc cacaggtagg ccgccaggac
     2401 gcggccgccg tccagcggcg ccccgggcaa catgttgaac acacccagca acaggttgat
     2461 cgcggcgagc cacgcggcca ccgctgcgac gatggccggc agccccgccg cctgggcaat
     2521 agccgccgcg gcgccgaaga ccgcggccag ccccacgctc atcgcgggac cggcgatcgc
     2581 gatgcggaat tccgcgcccg gcgtcttcgc gtcggaattt aggcgggcca cgccgcccaa
     2641 cagccacagc gtcacgctag acacctcgac gccggcgcgg cgggcgacga ccgcgtgcga
     2701 cagttcatgg gccagcagcg acgccagcag taccgcagcg ccgccgacgc cggcgagcca
     2761 gtagatcgcc gcggggtatc cgggggcggt gtggggcagc gtggcggcca ggctccaggt
     2821 gaacaaccac aggatcacca gcacggtcca gttcactttc accggacagc cgaacacccg
     2881 tcccagcgga atgtcctcgc gcatcgtcga tttcaccttc tcgtgtcgtg tgttcgtcgg
     2941 cgctcacagc gagtgggcgg tacgcgatgt gcggcggcgg tgatcatcgt gggttgctcc
     3001 tgtgattccg cgcggctggc tggcgttgtc gatgtcgtga cattgatgat gcggcgggcg
     3061 agcacagccc ggggagagcc gtacgtcagt tgatgagcgg cccaaagtca ccgggtgggc
     3121 gtgggcggcc ggcgccgcgg gtcgccacgc gggcgtcatg gatctcgcgg tggttgatcg
     3181 cggagatgac gtcggccggt cggccggtcg atcgcgcggc ggtgtcgagc ccgcgctcat
     3241 caattttcgc ctggcttttg ggactcctgg agcctctcgg ccggaacagc caccggtggc
     3301 cgacgaccgt ggaaagccct gtgccaaccg ctgtgacacc aatggcgcgc cgggatcact
     3361 ctcatggacg agccaggtga cctcgtcctc ccagtcgtgg ggccacccga cggcgaggaa
     3421 cgggaacggg cccccgcagc cgacgctcgc cccatttaag gggccgccca gcatcggact
     3481 ctggccgctc tggccaccgg cgagggtgaa ccgttgcccc gccccgtcct cgcagaggag
     3541 cagccaccag tcgcccttga tgcgaggcag gccaagcgca tcggccaact ccttggcggc
     3601 gggcccggac ttcttgactt tcatcagctt cttggtcatg taaatcgtag cttactcaaa
     3661 aacaatgcag cggaacatat ttctagctcg atatcccaac cattctgggg cacaaaacct
     3721 ttgtggtaca gcgcaccgcc attttgcggc acaatggtag atgggccttc tcccgtcggt
     3781 gggcgaatcc cctccgctgc tccataaatg tcctcgtcac ggggccggtc ccgctcggcc
     3841 gccgcgaagg gcatctgact ggacgggcat cgtcgctgtc gacgatcgcg cggaccagtc
     3901 cgtagtagaa ggtggcgttg gccac
//
LOCUS       DASWBF010000218         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_415894,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000218 DASWBF010000000
VERSION     DASWBF010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415894"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1751
                     /locus_tag="VF874_07700"
     CDS             <1..>1751
                     /locus_tag="VF874_07700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX9175968.1"
                     /translation="AMWKILRAQGVFVGRGAPGKVAFLYTGQGSQYVNMLAELRQLEP
                     LIAATFDEADKVMAPLLGKPLTEYIFADTADQATAERLAKQLMQTEITQPAVLATDLA
                     LTRMLGAYGVRPDMVMGHSLGEYGALVAAGALSFDASLEAVSARGREMASLSVDDNGA
                     MAAVFAPLAEIERIVAETDGYVVVANINSTGQAVIGGATDAVERAVAAFQAAGMNASR
                     IPVSHAFHTEIVAPVSEPLKASLRRLDVRPPSLPIVSNVTGDFYPADADADTMLDLLG
                     RQVASPVRFIHGLRTLYAAGARVFVEVGPKKALHGFVEDVLSDSEEGVLALFTNHPKQ
                     GDVASFNQALCGLYAAGLGYAEQAPAKLAAKTETAMADVAGPAATMTVAQFAELGRHL
                     VDVVQHGVHPQPAVTGSAPRATEPVVVTGAALGLPGGERVFDDENITRILDGQQFIDT
                     IPHKLRREMLDRHITRLVKRDSGDPTFETIDSESEVIKLAGRSAPLDVVSEFGVDTAR
                     DAALDTSTRLAIGAGFDALRDAGIPLVMHYKTTTLGTKLPDRWGLPESMRDDTGVIFA
                     SAFPGLDNFAKDLERYY"
BASE COUNT          283 a          606 c          604 g          258 t
ORIGIN      
        1 ggccatgtgg aagatcttgc gcgcccaggg cgtgttcgtc ggccgcggcg ccccgggaaa
       61 ggtcgcgttc ctctacaccg gccagggttc gcagtacgtc aacatgctcg ccgagctgcg
      121 tcagctcgag ccgctgatcg ccgcgacgtt cgacgaagcc gacaaggtga tggcgccgct
      181 gctgggcaag ccgttgaccg agtacatctt cgccgacacc gccgatcagg ccaccgcgga
      241 acggctggcc aagcagttga tgcagaccga gatcacccag cccgcggtgc tggcaaccga
      301 tctcgcgttg acgcgcatgc tcggcgcgta cggcgtccgg cccgacatgg tgatgggcca
      361 cagcctcggc gagtacggcg cgctggtggc tgcgggtgcg ctgtcgttcg acgcctcgct
      421 ggaggcggtg agtgcccgcg ggcgcgagat ggcgagcttg tccgtggacg acaacggcgc
      481 gatggccgct gtcttcgccc cgctggccga gatcgagcga atcgtcgccg agaccgacgg
      541 gtacgtcgtc gtcgccaaca tcaacagcac cggccaggcc gtcatcggcg gggccaccga
      601 cgcggtcgag cgtgcggtcg ccgcgtttca ggcggccggg atgaacgcgt cgcggatccc
      661 ggtcagccac gcgttccaca ccgagatcgt cgcgccggtg agcgaaccgc tgaaggcctc
      721 gttacgccgg ctcgacgtgc ggccaccgag cctgccgatc gtgtcgaacg tgaccggtga
      781 cttctacccg gccgacgccg acgccgacac catgctggac ctgctcggca ggcaggtggc
      841 gtcaccggtc cggttcatcc acgggctgcg caccttgtac gcggcaggcg cccgcgtctt
      901 cgtcgaggtc ggccccaaga aagcgctgca cggattcgtc gaggacgtgc tcagcgacag
      961 tgaagaaggt gtgctggcgt tgttcaccaa ccatccgaag cagggcgacg tcgcgtcgtt
     1021 taaccaggcg ctgtgcgggc tgtacgccgc cgggctgggc tatgccgagc aggcgcccgc
     1081 caagctcgcc gctaagacgg agaccgcaat ggcggatgtc gccggcccgg cggcgacgat
     1141 gacggtcgcg cagttcgccg agttgggccg ccacctcgtc gacgtcgtgc aacacggcgt
     1201 ccacccgcaa ccagccgtca ccggctcggc accgcgcgcc acggagccgg tggtggtaac
     1261 cggcgccgcg ctcggcctgc ccggcggcga gcgggtcttc gacgacgaga acatcacccg
     1321 catcctggac ggccagcagt ttatcgacac gattccgcac aagctgcgcc gcgaaatgct
     1381 cgaccgccac atcacccggc tggtcaagcg cgactccggg gacccgacgt tcgagaccat
     1441 cgacagcgag tcggaggtca tcaagctggc cgggcgcagc gcgccgctgg acgtggtgag
     1501 cgagttcggc gtcgacaccg cccgcgacgc cgcgctggac accagcaccc ggctcgcgat
     1561 cggggccggt ttcgacgcgc tgcgcgacgc cgggattccg ctggtgatgc actacaaaac
     1621 cacgacgctg ggcaccaagc tgcccgaccg gtggggcctg ccggagtcga tgcgcgacga
     1681 caccggcgtg atcttcgcgt ccgctttccc gggtttggac aacttcgcca aggatctgga
     1741 gcgctactac a
//
LOCUS       DASWBF010000219        10144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1268629,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000219 DASWBF010000000
VERSION     DASWBF010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268629"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF874_07705"
     CDS             <1..663
                     /locus_tag="VF874_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175969.1"
                     /translation="VQEERDFHVLNPPGLGAPPACGGQLRSADGLVVHRRDGAPLQIV
                     NRRPVTSPAWTAIEVARSLRRPRALATLDAALRSGTCNRAELWRAAIEQAGRRGIVAV
                     RELLPLADERAESPMESEARLAMIDGGLPIPELQYEIIDGNGEVRRVDFAWPDERVAV
                     EYEGLDWHSGAEAMRRDRTRQTALMDVGWIVIAIVFEDVRYRAWDFVARIDRQLRRAH
                     AA"
     gene            complement(723..3275)
                     /locus_tag="VF874_07710"
     CDS             complement(723..3275)
                     /locus_tag="VF874_07710"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HEX9175970.1"
                     /translation="MALTPDDRPVLPIPVVPPKPSASALRRVLRRARDGVALNVDEAA
                     VAMTARGDDLADLCASAARVRNAGLSGRAGPLPISYSRKVFIPVTHLCRDQCHYCTFV
                     TVPGKLRAAGAGMYMEPDEIVEVARRGAELGCKEALFTLGDRPEARWPEAAQWLHERG
                     YDTTLDYVRAMSIRVLEETGLLPHLNPGVMSWSELSRLKPVAPSMGMMLETTSRRLFE
                     TKGLAHYGSPDKDPAVRLRTLTDAGRLSIPFTTGLLVGIGETLVERAETLHAIRKSHK
                     EFGHVQEVIVQNFRAKPGTAMRAAPDAGFDDFVATVAVARLVLGPKMRIQAPPNLVSR
                     SECLALIGAGVDDWGGVSPLTPDHVNPERPWPALDELAAVTAEAGFELVQRLTAHPPY
                     VLAGAAWIDPRVRGHVSALADPATGFARDVNPVGRPWQEPDAALESSGRVDLNAAIDT
                     EGRLSAARSDLGSAFGDWESVREKVAELAARAPERVDADVLAALRSAERDPAGCSDAE
                     YLALATADGPGLDAVAAFANSLRRDVVGDDVTFVVNRNINFTNICYTGCRFCAFAQRK
                     GDADAYTLSYDEVGERAWQAYVDGATEVCMQGGIDPELPATGYADLVRAVKKRVPSMH
                     VHAFSPMEIVNGAAKSGLSIRDWLIGLREAGLDTIPGTAAEILDDEVRWVLTKGKLPT
                     SAWIEVVTTAHEVGLRSSSTMMYGHVDAPRHWIGHLRILRDIHDRTGGFTEFVPLPFV
                     HQSSPLYLAGGARPGPTHRDNRAVHALARIMLHGRISHIQTSWVKLGVERTQVMLNGG
                     ANDLGGTLMEETISRMAGSEYGSAKTVAELVEIAAGIGRPARQRTTTYLLAA"
     gene            complement(3328..3717)
                     /locus_tag="VF874_07715"
     CDS             complement(3328..3717)
                     /locus_tag="VF874_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175971.1"
                     /translation="MDSVVGVIVLVLLAVDGVISAVLGGLFVQVRIGPVPFPVSALIS
                     GLVNALLVWAGLHWTSSLGVAATALWTFLLTLAAMTLSGPGGDVVFGGPGFDQWSAAA
                     LLILGTLPAGVLLWRRRQAAGQVGGLR"
     gene            complement(3919..4770)
                     /gene="mshB"
                     /locus_tag="VF874_07720"
     CDS             complement(3919..4770)
                     /gene="mshB"
                     /locus_tag="VF874_07720"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HEX9175972.1"
                     /translation="MSESPRLLFVHAHPDDETLTTGATIAHYASRGADVRVVTCTLGE
                     EGEVIGDRYALLAVDHADQLGGYRIGELTEALAILGAGRPNFLGGPGRWRDSGMAGTP
                     LRRHPRFVDAGDPAVHELTAIIDGFRPHVVVTYDPNGGYGHPDHIHAHTVTTAAVAQS
                     GWAVPKFYWAVLSTSAVNAAFAAIDDIPESWIRAEPGDFDFGYPDDEIDAVVDASEHL
                     PAKVAALTAHATQVSVAPNRRSFALSNNLALPIGGQEHYVLAAGTPGPLDDRGWETDL
                     LAGLDLG"
     gene            complement(4773..6611)
                     /locus_tag="VF874_07725"
     CDS             complement(4773..6611)
                     /locus_tag="VF874_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HEX9175973.1"
                     /translation="MPRGSLRAVALAGALAIVSACTVSPPPAPQSTDTSQNTAPPPAR
                     AFQIIMGIDSIGAGFNPHLLSDQSPVNAAVASLVLPSAFRPVPDPSTATGSRWEMDST
                     LLLSAEVTSNDPFTVTYKIRPEAQWTDNAPIGADDFWYLWRQMVTQPGVVDPAGYNLI
                     TSVQSIDGGKQAVVTFSAPYPAWRELFTDILPAHIVKDAPGGFAAGLARALPVTGGQF
                     RVENIDPQRDEILLARNDRFWAAQALPDHILMRRAGAPAALADSMRNGDTQVAQVHGG
                     AAAFAQLSAIPDVRTARIMTPRTMQVSLRAVQPKLSDVRVRQAILGLLDVDLLAAVGA
                     GSDNTVTLAQAQVRAPSDPGYVPTAPPALSKEAALALLMRAGYDIDAGDPVTPAPPSP
                     TPESTRGQISKDGVPLTLVIGVAANDPTSVAVANTAADQLRNVGIAASVQPLDPAVLY
                     GDAVPNNRVDAVVGWHRAGGDLATEMASRYGCTGLGGGPTPYATTPSVTPTTTMPTTS
                     ASPITPASGPAPEPGAPVPVPNNITGVCDPALQPRIDAGLQGHVPIGEVIDFIEPKLW
                     QMATVLPILQDTTIVAAGPRVENVSLSGAVPVGIVADAGRWVKMPQ"
     gene            complement(6648..8531)
                     /gene="typA"
                     /locus_tag="VF874_07730"
     CDS             complement(6648..8531)
                     /gene="typA"
                     /locus_tag="VF874_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX9175974.1"
                     /translation="MQFRNVAIVAHVDHGKTTLVDAMLRQSGALTHRGDDAKERVMDT
                     GDLEREKGITILAKNTAVHRHHPDGSVTVINVIDTPGHADFGGEVERGLSMVDGVLLL
                     VDASEGPLPQTRFVLRKALAAHLPVILVVNKTDRPDARIAEVVHASHDLLLDVASDLD
                     DEAAKAAEHALGLPTLYASGRAGVASTTAPPDGEIPDGTDLDPLFDVLLEHIPPPSGN
                     PDAPLQALVANLDASPFLGRLALVRVYNGRLRKGQTVAWLREDGVTSAKITELLATEG
                     IERKPTDEAQAGDIVAVAGFPDIMIGDTLADPTEPKPLPRITVDEPAISVTIGTNTSP
                     LAGKVTGHKLTARMVRSRLDTELVGNVSIRVVDIDRPDAWEVQGRGELALAVLVEQMR
                     REGFELTVGKPQVVTRTIDGALHEPFEALTIDCPEEYVGAITQLMAARKGRMVEMANH
                     AAGWVRMDFLVPSRGLIGFRTDFLTETRGTGIANGVFDGYHPWAGDIKSRHTGSLVSD
                     RAGIVTPFALLQLADRGTFLVEPGQDTYEGMVVGVNPRAEDLDVNVTREKKLTNMRSS
                     TADVIETLAKPIDLDLEKAMEFCAVDECVEVTPEIVRVRKAELDATTRARSRSRAKAA
                     GRA"
     gene            complement(8572..9189)
                     /locus_tag="VF874_07735"
     CDS             complement(8572..9189)
                     /locus_tag="VF874_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175975.1"
                     /translation="MTLWRQCWLFMIGSAFFAVGTAPGFASVASATATNALCFVGSWF
                     FTSAAFIQLMQSRSPASRSWADPSARADWLSSAIQFVGTLLFNLSTGAALLAHRVPAQ
                     RRFVWLPDANGSVAFLVSGVLAVVAVTLSIGAFELSSRDWQAAWVNMLGSIAFGASAV
                     GAFVNRAGATADALLANVGTFFGALCFLAAAYLLLPNRSAPVAAA"
     gene            9272..>10144
                     /locus_tag="VF874_07740"
     CDS             9272..>10144
                     /locus_tag="VF874_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX9175976.1"
                     /translation="MGAGMEQTKSPLTVIGNPSSAASYAAGQDRAPRALRDAGLIPAL
                     IAAGRKVVDTGDLPQQIWRPDRVNPRAQNVSAVVENLSLLRMRLTPVLRDGGDVLVLG
                     GNCTGALACVAAMRDATERPPALLYIDRHLDCNTPESTTDGALDWMGMAHGFDLPGTV
                     DAVCRALGHRPLLDPDGVAFLGVDLALATEWERRQVQERSLFVVSSEELSRRPGEATA
                     DALARLPAGPLIVHVDVDVLDFVDAPLAENTDGRNSGPTLDDLRITLDQVKRCGGARV
                     LSIGELNPTRSAGDP"
BASE COUNT         1591 a         3534 c         3458 g         1561 t
ORIGIN      
        1 gtccaagagg agcgtgactt ccatgtgctc aatccgccgg gcctgggggc acctcccgct
       61 tgcgggggac agttacgttc cgccgacggt ctcgtggtac atcggcgtga cggtgcgcca
      121 ctccagatag ttaaccgccg ccctgtcacg tcgcctgctt ggactgccat cgaggtggct
      181 cgaagcctgc gacgcccgcg ggcgctggcc acactggacg ccgcgttgcg cagcggcacg
      241 tgcaatcgcg ccgagctatg gcgcgcggcc atcgagcagg ccggccgccg aggcatcgtc
      301 gcggttcgcg agttgttgcc gctcgccgac gaacgggccg agtccccgat ggagagcgag
      361 gctcggctgg cgatgatcga cggcggcctc ccgattccgg agctgcaata cgagatcatc
      421 gacggaaacg gggaagtacg gcgtgtggac ttcgcatggc cggatgagcg ggtggcggtc
      481 gagtacgagg gactcgactg gcacagcggc gccgaggcca tgcgcagaga ccgcacgcgt
      541 cagacggcgc tgatggatgt tggctggata gtcatcgcga tcgtcttcga ggatgtgcgg
      601 tacagggcat gggacttcgt cgcgcgtatc gaccggcagt tgcgccgcgc tcacgccgcg
      661 tgagcgtgcg cagactgcgg gcgaaatgcg gcgtgtcgag ctacaaacac gcgcgctcgc
      721 ggttaagcgg cgagcaggta ggtcgtcgtt cgttgacgtg cgggtcggcc gatgcctgcg
      781 gcgatctcca ccagctcggc gacggtcttt gccgatccgt actcggagcc ggccatccgc
      841 gagatcgtct cctccatcag cgtgccgccc aggtcgttgg cgccgccgtt gagcatgact
      901 tgtgtccgct cgacgccgag cttgacccag ctggtctgga tgtgagagat cctgccgtgc
      961 agcatgatcc gcgccagcgc gtgcaccgcg cggttgtcgc ggtgcgtcgg gcccgggcgc
     1021 gcgccgccgg ccagatacag cggcgagctc tggtgcacga acggcagcgg cacgaactcg
     1081 gtgaaaccgc ctgtgcggtc gtggatgtcg cgcagaatcc gcagatgccc gatccagtgc
     1141 ctgggcgcgt ccacgtggcc gtacatcatc gtcgagctgg accgcagccc gacctcgtgc
     1201 gcggtggtga cgacctcgat ccacgcagac gtcggcagct tgcccttggt cagcacccag
     1261 cgcacctcgt cgtcgaggat ctcggcggcc gtgccgggaa tcgtgtccag ccccgcctca
     1321 cgcaggccga tcagccaatc ccgaatactc aagccgcttt tcgcggcgcc gttgacgatc
     1381 tccatcggag aaaacgcatg cacgtgcatg ctgggcaccc gcttcttgac cgcccgcacc
     1441 agatcggcgt acccggtggc gggcagctcg ggatcgatgc cgccctgcat gcacacctcg
     1501 gtggcgccgt cgacgtaggc ctgccacgcg cgctcgccga cctcgtcgta ggacagcgtg
     1561 taggcgtcgg cgtcgccctt gcgctgggcg aacgcacaga accggcagcc ggtgtagcag
     1621 atgttcgtga agttgatatt gcggttcacc acgaaggtca cgtcgtcgcc gacgacgtcg
     1681 cgtcgcaacg aattcgcaaa cgccgcaacg gcatccaaac ccggcccgtc ggccgtcgcc
     1741 aacgccagat actcggcgtc actacacccg gcgggatcgc gctctgccga ccgcagcgcc
     1801 gccaacacgt cggcgtcgac ccgctcgggt gcgcgcgcgg ccaactcggc caccttctca
     1861 cgcaccgact cccagtcgcc gaacgcgctg cccagatcac tgcgcgctgc actcaaccga
     1921 ccctcggtgt cgatggcggc attcaggtcg acgcggccgg aggactccag cgccgcgtcg
     1981 ggctcctgcc atggccggcc cacgggattg acgtcacgcg cgaaaccggt cgcagggtcg
     2041 gccagcgccg aaacatgtcc gcgcacccgc ggatcgatcc atgccgctcc cgccaacaca
     2101 tacggcggat gcgcagtcag ccgctgtacc aactcgaaac cggcctctgc cgtcaccgcg
     2161 gccaactcgt ccagcgccgg ccacggtcgt tcgggattga cgtgatcggg cgtcagcggc
     2221 gacaccccac cccagtcgtc gaccccggcg ccgatcagcg ccagacactc gctgcgcgac
     2281 accaggttcg gcggcgcctg gatgcgcatc ttcggcccca gcaccagccg cgccaccgcc
     2341 accgtcgcga cgaagtcgtc aaagccggcg tcgggcgccg cacgcatcgc ggtgccgggc
     2401 ttggcgcgga agttctgcac gatcacttcc tggacgtggc cgaactcctt gtgcgacttg
     2461 cggatcgcat gcaacgtctc ggcgcgttcg acaagcgtct cgccgatgcc caccagcagc
     2521 ccggtggtga acgggatcga cagccggccc gcatcggtga gtgtgcgcag ccgcaccgcc
     2581 gggtccttgt cggggctgcc gtaatgcgcc aggcctttgg tttcgaacag ccgccgcgac
     2641 gtggtctcca acatcatgcc catcgacggc gcaacaggct tcagccgtga caactccgac
     2701 caactcatga cgccgggatt caggtgcggc agcaggccgg tctcctcgag cacccggatc
     2761 gacatcgccc gcacgtagtc cagcgtggtg tcgtaacctc gctcatgaag ccattgcgca
     2821 gcctccggcc atcgcgcctc cggacggtcg ccgagtgtga aaagcgcttc cttgcaacct
     2881 aattcggctc cgcggcgggc aacctcgacg atctcgtcgg gctccatgta catgccggcg
     2941 cccgccgcgc ggagcttgcc cgggaccgtc acgaacgtgc agtagtgaca ctggtcgcgg
     3001 cacaggtgag ttacggggat gaagaccttg cgcgagtagc tgatcggcag cggcccggca
     3061 cgccccgaca gccccgcatt ccgcacccgt gccgcgctgg cgcacaggtc ggcgaggtcg
     3121 tcgccacggg ccgtcatcgc aacagcggcc tcgtcgacgt tcagcgccac cccgtcacgc
     3181 gcgcgccgca acacgcgccg caacgccgat gcgctcggct tcgggggcac cacggggatg
     3241 ggcaggacgg gccgatcgtc gggggtcaac gccacgctcg tgtaactcct gcggggtcca
     3301 gaatcttcca ggcgcctgcc atgcgtgtca gcgtagaccg ccgacctgcc ccgcagcttg
     3361 tcggcgtcgc catagcagga ccccggccgg caatgtgccg aggatcagca gcgcggccgc
     3421 actccactgg tcaaaaccgg ggccgccgaa caccacatct ccgcccggac cgctcagcgt
     3481 catcgccgcg agcgtgagaa ggaacgtcca cagcgccgtc gcagccaccc ccagcgacga
     3541 ggtccagtgc agccccgccc acaccagcag cgcgttcacc aggccgctga tcagcgcact
     3601 gaccggaaac ggcacgggac cgatgcggac ctggacgaac agccccccca acaccgccga
     3661 gatcaccccg tcgacggcga gcagcaccag cacgatcacg cccactacgg aatccaccgg
     3721 ccgaccactt gggtcgtcgg tgggtaaaga accagtcagg tcggaatgct gtcgagcacg
     3781 ctgaccagcg agccgaccac ccactggaaa ctatcggcgt gccactgcca gtactccagt
     3841 tgcggatcca gatccgggtc catgccgtcc ctcccttgcg ccgctggtgt ggtcgaagct
     3901 tacgcgcagc aacgtcggct atccaaggtc gagcccggcc agcagatcgg tttcccagcc
     3961 gcggtcgtcc agcggtccgg gtgtgcctgc ggccagcacg taatgctcct gaccgccgat
     4021 cggcagtgcg aggttgttgg acaaggcgaa cgatcgccgg ttgggcgcga cgctgacctg
     4081 ggtggcgtgg gcggtgagtg cggcgacctt cgccggaagg tgctcgctcg cgtcgacgac
     4141 ggcgtcgatc tcgtcgtcgg gatagccgaa atcgaagtcg cccggctcgg cccggatcca
     4201 gctctccggg atgtcgtcga tcgcggcgaa cgcggcgttg acggccgagg tcgacagcac
     4261 agcccagtag aacttcggta cggcccaacc ggattgggcg acggcggccg tggtgacggt
     4321 gtgagcatgg atgtggtccg ggtgcccgta cccgccgttg gggtcgtagg tgacgacgac
     4381 gtgcgggcga aacccgtcga tgatcgccgt cagctcgtgc acggcggggt cgcccgcgtc
     4441 gacgaaccgc gggtgacggc gaagcggcgt gcccgccatg cccgagtccc gccagcgacc
     4501 gggaccgccg aggaagttcg gccggccggc accgaggatc gccaacgcct ccgtcagctc
     4561 accgatgcgg tagccgccga gttgatcggc atggtcgacg gccaacagcg cgtagcggtc
     4621 cccgatcacc tcgccttcct caccgagcgt gcaggtcacc actcgcacgt cggcgccgcg
     4681 gctcgcgtag tgggcgatgg tcgcgccggt ggtcagcgtc tcgtcgtcgg ggtgggcgtg
     4741 gacgaacagc agccggggag attcgctcat ggctactgcg gcatcttcac ccagcgaccg
     4801 gcgtcggcga cgatcccgac cggcaccgca ccggacaggc tgacgttctc gacgcgcggc
     4861 ccggcggcga cgatcgtggt gtcctgcagg atcggcagca cggtcgccat ctgccacagc
     4921 ttgggctcga tgaaatcgat cacctcgccg atggggacgt ggccctgcag ccccgcgtcg
     4981 atgcgcggct gaagcgccgg gtcgcacacg ccggtgatgt tgttgggcac cggaaccggc
     5041 gcgcccggct cgggtgcggg ccccgacgcc ggcgtgatcg gcgaagccga ggtggtcggc
     5101 atggtcgtcg tcggcgtcac gctcggtgtc gtcgcgtacg gcgtcggccc tccgccgagc
     5161 ccggtgcagc cgtagcgcga cgccatctcg gtggctagat caccgccggc gcgatgccag
     5221 ccgacgaccg cgtccacccg gttgttcggc accgcgtcgc cgtacagcac cgcagggtcc
     5281 agcggctgaa ccgacgccgc gatcccgacg ttgcgcagct ggtcggccgc ggtgttggcc
     5341 accgcgacag atgtcgggtc gttcgccgcg accccgatca ccaacgtcag cggaacgccg
     5401 tccttgctga tctgcccgcg cgtggactcc ggcgtcggcg agggcggcgc cggcgtcacc
     5461 gggtcgcccg cgtcgatgtc gtacccggcc cgcatcagca gcgcgagtgc ggcttccttg
     5521 ctcaacgcgg gcggcgcggt cggcacatag ccggggtcag acggtgcgcg cacctgcgcc
     5581 tgcgccagcg tgacggtgtt gtcgctgcct gccccgacgg ctgccagcag atccacgtca
     5641 agcaagccga ggatcgcctg gcgcacccgc acgtcggaca gcttcggctg aaccgcacgc
     5701 agcgacacct gcatggtccg cggcgtcatg atcctggccg tgcgcacgtc cgggatcgcg
     5761 gacagctgcg cgaaagccgc tgcgccgccg tgcacttggg cgacctgggt gtcgccgttg
     5821 cgcatcgagt cggccagcgc cgccggagcg cccgcccgcc gcatcaagat gtggtcgggc
     5881 agcgcctgcg ccgcccagaa ccggtcgttg cgggccagca ggatttcgtc gcgctgcggg
     5941 tcgatgttct cgacgcggaa ctgcccgccg gtcaccggca gcgcccgcgc cagcccggcg
     6001 gcgaaaccgc ccggtgcgtc cttgacgatg tgcgcgggca ggatgtcggt gaacagctct
     6061 cgccatgccg gatacggcgc cgagaacgtc accaccgcct gcttgccgcc gtcgatcgac
     6121 tgcaccgacg tgatcaggtt gtacccggcc gggtcgacca cgccaggttg ggtgaccatc
     6181 tgccgccaca agtaccagaa gtcgtcggca ccgatcggcg cgttgtcggt ccactgggcc
     6241 tcgggccgga tcttgtaggt gacggtgaac gggtcgttgc tggtcacctc ggccgacagc
     6301 agcagtgtgg agtccatttc ccagcgggag ccggtggccg tcgacggatc cggcaccggc
     6361 cggaacgcgc tcggcaacac caacgacgcg accgccgcgt tcaccggcga ctggtccgac
     6421 agcaggtgcg ggttgaatcc ggcgccgatc gagtcgatgc ccatgatgat ctggaacgca
     6481 cgtgcgggcg gaggcgccgt gttctgcgac gtgtcggtgc tctgcggcgc gggcggcggg
     6541 ctcaccgtgc aggccgacac gatcgccagc gcgccggcca gggcgacggc acgcagggat
     6601 cctctcggca cgccctacag ggtaatgatg accacaagac gcgcccgcta agcgcggcca
     6661 gccgccttgg cgcgtgaccg gctgcgggcc cgtgtcgtgg cgtccaactc ggccttgcgc
     6721 acgcgcacga tctcgggcgt cacctcgaca cactcgtcga ccgcgcagaa ctccatcgcc
     6781 ttctcgaggt cgagatcgat cggcttggcc agcgtctcga tgacgtccgc ggtggagctg
     6841 cgcatgttcg tcagcttctt ctcccgggtg acgttgacgt cgaggtcctc cgcgcgcggg
     6901 ttcacgccga cgaccatgcc ctcgtaggtg tcctgcccgg gttcgaccaa gaacgtgccg
     6961 cggtcggcaa gttgcagcaa cgcgaacggt gtgacgatgc ctgcgcggtc ggacaccagt
     7021 gagccggtgt ggcgggactt gatgtcgccc gcccatgggt gatacccgtc gaacaccccg
     7081 ttcgcgatcc cggtgccgcg ggtttcggtg aggaaatccg tgcggaaccc gatcaggccg
     7141 cggctcggca ccaggaagtc catccgcacc cagcccgccg cgtggttggc catctcgacc
     7201 atccggcctt tacgcgcggc catcagctgg gtgatcgcgc cgacgtactc ctcggggcag
     7261 tcgatggtca gcgcctcgaa cggctcgtgc aatgcgccgt cgatcgtgcg ggtgaccacc
     7321 tgcggcttgc cgacggtcag ctcgaagcct tcccggcgca tctgctcgac gagcaccgcc
     7381 aatgccagct cgccgcggcc ctgcacctcc caggcgtcgg gacggtcgat gtcgacgacc
     7441 cggatcgaca cgttgccgac gagctcggtg tccagccgcg accgcaccat ccgcgcggtg
     7501 agcttgtggc cggtgacctt gccggccagc ggtgacgtgt tggtgccgat cgtcaccgag
     7561 atcgccggct cgtcaacggt gatgcgcggc aacggttttg gctcggtcgg gtcggccagc
     7621 gtgtcgccga tcatgatgtc gggaaaaccg gcgaccgcga cgatgtcgcc ggcctgcgcc
     7681 tcgtcggtgg gcttgcgctc gatgccctcg gtggccagca gctcggtgat cttcgcgctc
     7741 gtgacgccgt cttcacgcag ccacgcgacg gtctggccct tgcgcagcct gccgttgtag
     7801 acccgcacca gagccaagcg gccgaggaac ggcgacgcat ccaggttcgc caccagcgcc
     7861 tgcagcggcg cgtcggggtt gccggacggc ggcgggatgt gctcgagcag cacgtcgaac
     7921 agcgggtcca aatcggtgcc gtccggaatt tcgccgtcgg gcggcgcggt ggtgctggcc
     7981 accccggcgc ggcccgacgc gtacagcgtc ggcagcccga gcgcatgctc ggcggctttc
     8041 gccgcttcgt cgtcgagatc ggacgccacg tcgagcagca ggtcgtggct ggcgtgcacc
     8101 acctccgcga tgcgcgcgtc tggccggtcg gtcttgttga cgacgaggat caccggcagg
     8161 tgcgcggcca gcgccttgcg cagcacgaag cgggtctgcg gcagcggccc ctcggacgcg
     8221 tcgaccagca gcagcacccc gtcgaccatc gacagcccgc gctccacctc gccgccgaag
     8281 tcggcgtggc cgggggtgtc gatgacattg atcacggtga cgctgccgtc ggggtgatgc
     8341 cggtgcaccg cggtgttctt ggcgaggatc gtgatgcctt tttcgcgttc caggtcgccg
     8401 gtgtccatca cccgctcttt ggcgtcgtca ccgcggtgcg tcagcgcgcc ggactgccgc
     8461 agcatcgcgt cgaccagcgt cgtcttgcca tggtcgacgt gcgcgacgat cgcgacgttc
     8521 cggaactgca ctggctgatt gtggcagcgc agtggtcgat cgcgaaaacg gtcaggcagc
     8581 agcgaccggc gccgagcggt tcggcaagag caggtaggcg gcggcgagaa agcacagcgc
     8641 tccgaagaac gtgcccacgt tggccagcag cgcgtcggcg gtggctcccg cgcggttgac
     8701 gaacgctccg accgctgacg cgccgaacgc gatcgagccg agcatgttca cccaggcggc
     8761 ctgccagtcc cgcgagctca gctcgaacgc tccgatgctc agtgtcaccg ccacgaccgc
     8821 caacacaccg ctgaccagga atgccaccga cccgttggcg tcgggcaacc acacgaagcg
     8881 gcgctgcgcc ggtacccggt gggccagtaa tgccgcgccg gtgctgaggt tgaacagcag
     8941 ggtgccgacg aactggattg ccgacgacag ccagtcggca cgcgcggacg ggtctgccca
     9001 cgaccggctt gccggggatc gactctgcat cagctgaatg aatgcggcgc tggtgaaaaa
     9061 ccatgagccg acgaagcaca gcgcgttggt ggccgttgcg ctggcgacac tcgcgaatcc
     9121 gggtgccgtg ccgaccgcga agaacgccga cccgatcatg aagagccagc actgccgcca
     9181 gagcgtcacg cggccgattg tctcaatgca gggcacatgg ccgtcgcgtc gagtccgttg
     9241 cagcggagaa ggcgcgctag cctcggggac cgtgggcgca ggcatggagc agaccaagtc
     9301 accgctgacg gtgatcggaa accctagcag tgcagccagt tacgcggccg gccaggaccg
     9361 cgcgccgcgc gcgctgcgcg atgccgggtt gatccctgcg ttgatcgcgg ctggacgaaa
     9421 agtcgtcgat acaggagatc tgccgcagca gatctggcgg cctgatcgag tcaatccccg
     9481 cgcgcagaat gtgtctgccg tggtcgaaaa cctgtccctg ttacgcatgc ggctgacgcc
     9541 ggtcttacgt gacggcggtg acgtgctcgt gctcgggggc aactgcaccg gcgcgttggc
     9601 ctgtgtggcg gcgatgcggg acgcgacgga acgccctccc gccctgcttt acatagaccg
     9661 ccatctggac tgcaacaccc ccgaaagcac caccgacggg gcgctcgact ggatgggcat
     9721 ggctcacggg ttcgacctgc ccgggacggt cgacgcggtg tgtcgtgctc tggggcatcg
     9781 accccttctc gacccggacg gggtggcgtt cttgggcgtc gatctcgctc tcgccaccga
     9841 gtgggagcgc cgacaagtgc aggagagatc gctgttcgtt gtgagttcgg aagaactgag
     9901 ccgccgcccc ggcgaggcga ctgccgacgc gctggctcgg ctgcccgccg ggccgctgat
     9961 cgtccacgtg gacgtggatg tcctggattt cgtcgacgcg ccgctggccg agaacaccga
    10021 tggacgcaat tccgggccca cactcgacga cctgcgcatc acactggatc aggtcaagcg
    10081 ctgcggaggc gcacgcgtcc tgtcgatcgg cgaactgaat cccacccgat ccgcgggcga
    10141 cccg
//
LOCUS       DASWBF010000220        11004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_484613,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000220 DASWBF010000000
VERSION     DASWBF010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11004
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484613"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..891)
                     /gene="truB"
                     /locus_tag="VF874_07745"
     CDS             complement(16..891)
                     /gene="truB"
                     /locus_tag="VF874_07745"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX9175977.1"
                     /translation="MSGLVVVDKPPGLTSHDVVGRCRRIFATRKVGHAGTLDPMATGV
                     LVIGVERATKILGLLTASTKSYAATIRLGQATTTDDADGDVVRTAAADEVTDAAINAA
                     IAALRGEIRQVPSAVSAVKVGGKRAYRLVREGQPVELAARTVRIDRFDALTIRRQQGV
                     VDVDVEVDCSSGTYIRALARDVGAALGVGGHLTALRRTRVGRFGLDQAHTLDDLADHP
                     CLSYSLDEACLMTFPRRDLNPDEAEFARHGRALAPAGIDGVYAATAPDGRVVALLRDD
                     GARTKSVVVLRPATL"
     gene            complement(888..1544)
                     /locus_tag="VF874_07750"
     CDS             complement(888..1544)
                     /locus_tag="VF874_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HEX9175978.1"
                     /translation="MLISTVVSDSVAAAEMYADPPDLKPLPDEEPLIERSVAKRRNEF
                     VTVRHCARLALGELGVPPAPILKGDKGEPCWPDGVVGSLTHCEGFRAAVVGRASEVRS
                     VGIDAEPHGVLPNGVLDAVSLPAERHELAAMPDELHWDRILFCAKEATYKAWYPLTRR
                     WLGFEDAHIVFDVNSDGISGEFVSQILIDPTALSGPPLTALRGRWSVRERLALTAIVL
                     "
     gene            complement(1544..2491)
                     /locus_tag="VF874_07755"
     CDS             complement(1544..2491)
                     /locus_tag="VF874_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9175979.1"
                     /translation="MWSVVATLWAVSDIHTGHTGNKPVTESLYPSSPDDWLIVAGDVA
                     ERTDDIRWSLDLLRRRFAKVIWVPGNHELWTTGKDPVQVFGRARYDYLVDMCDAMGIV
                     TPEHPFPVWTEQGGPATIVPMFLLYDYTFLPAGASSKSEGLAIARDRNVMATDEFVLS
                     SEPYATRDAWCRERVAYTRERLEQLDWMTPTVLVNHFPLVREPCEALFYPEFSLWCGT
                     TATADWHTRYNAVCSVYGHLHIPRTTWYDGVRFEEVSVGYPREWRRRKPYRWLRQVLP
                     DPEYAPGYLNEFGGHFVITPEMRANAERVRERIQQRASR"
     gene            2642..3226
                     /locus_tag="VF874_07760"
     CDS             2642..3226
                     /locus_tag="VF874_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEX9175980.1"
                     /translation="MATRLRLFAVLCALVAAVVVVVQSAPANLAATRNVQLRSTAVPM
                     DSPTTTIKWPVIDMVDPSPFVPCEDIPLDVIERLGLAFTPPEPEDGLRCHYDAANYQM
                     AIEPIIWRTYQQSLSPDAVETTVNGHRAASFWVMKPTDWNNKWWFSCMLMFETSYGVI
                     QQSLFFSPVYSDPDVDCPSENMARAQQLVPYYKF"
     gene            3229..4950
                     /locus_tag="VF874_07765"
     CDS             3229..4950
                     /locus_tag="VF874_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX9175981.1"
                     /translation="MTTTTQRPVGPRIRRMARRSGHRAMARLFGLPAPTTHYTVERGL
                     RIPMRDGVDLVADHYAPTDATKGTLLVRVPYGRGLPLSLLYARLYAARGYHVVMQSVR
                     GTSGSGGVFEPFIYEASDGADTVEWLRRQPWFTGRFATIGSSYLGTAQWALLTDPPPE
                     LAAAVVTAAPHDLRTSMWGTGAFRLTDVLGWSDVVSHQEHGSRLRGMIWAAGARRRLT
                     RAMAAVPLGESARSLLADGAAWFESWLEHHDPEAPFWEPYRFGAALTQVTVPVLLIGG
                     WQDSFLSQTLEQYQVLRGRGVDVGLTVGPWTHAQLTATGAGRVARESLEWLDRHLADA
                     PTGMARSPVRVYITGDDWVELPDWPPPTSPRVLFLAPGAALSDRPPPDTGGSSTFTYD
                     PADPTPILGGPLVLAAKGGYREDSRLARRADVLSFTSDPLPSDLYVVGHPVVELSHAA
                     DTPHVDVAVRVSEVDSRGRSRNVSDGYRRVTTSSEPGGVHVELEAVAHRFRAGSRIRL
                     LVAGGAHPRFDRNLGTGEPTLTAQRLVPATHTVHHGAAGASRLILPSTRQRPSAHGAP
                     DPVGDSA"
     gene            complement(4909..5469)
                     /locus_tag="VF874_07770"
     CDS             complement(4909..5469)
                     /locus_tag="VF874_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEX9175982.1"
                     /translation="MTATSSRPALKEWSAAVHALLDGRQTVLLRKGGIGEKRFEVAAQ
                     RFLLFPTVAHSHAERVREEHRDLLAPAAADSDDDHIMLRAGAKVVAAVSVSNPDGLTD
                     IEPLHIWTQESVREDRLDFRPKHRLTVLVVQACALTEPVRLARTSDYAGCTSWVDVPV
                     APDWGPPVHDDATLRGIADRVRRAVG"
     gene            complement(5466..5729)
                     /locus_tag="VF874_07775"
     CDS             complement(5466..5729)
                     /locus_tag="VF874_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX9175983.1"
                     /translation="MCRNITELRGLQPAATDEEIEAAARQYVRKVSGITRPSAANVDA
                     FEAAVAEVTATTSRLLSELPPRKQPPKSIPPLRRPEVRARLRA"
     gene            5752..6534
                     /locus_tag="VF874_07780"
     CDS             5752..6534
                     /locus_tag="VF874_07780"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9175984.1"
                     /translation="MADDDVLLIETADRVRTLTLNRPHVRNALSTKLQDTLFDALARA
                     ETDDDVDVVILTGTDPAFSAGVDLKEISAGLHRPKGRTGEDLSTVRWPSMTKPVIGAI
                     NGVCVTGGFELALSCDILIASENARFADTHARVGLLPGWGLSVRLPQVVGRGMAYRMS
                     LTGDYLGAYDALRVGLVTEVVLHDELMDTARAVAASIVNNNRAAVQALLASYHRIDEA
                     QTREAFAIEHESAENWLRTNASSADIAANREAVIKRGRSQVR"
     gene            6552..7124
                     /locus_tag="VF874_07785"
     CDS             6552..7124
                     /locus_tag="VF874_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9175985.1"
                     /translation="MNEAHQFCGSDEWRQMIRDVILPWALDGTDLGDEVLEVGPGYGA
                     TTDVLSCAVPKLTSVEIDDELAAMLTDRFAGSPVVSIVHGDACDLDYEVGHFSGAACF
                     TMLHHVAPAERQDRLFGEIARVLRPGAPLVASDSLASDELAAAHDGDTYNPVDPASLA
                     TRLERAGFTRVAVRTNEYGWAAVAVRAPTG"
     gene            complement(7121..8470)
                     /locus_tag="VF874_07790"
     CDS             complement(7121..8470)
                     /locus_tag="VF874_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9175986.1"
                     /translation="MRRLGKGVELTASGDSTASTRRIAALALPALVVLAAEPLYLLFD
                     TAVVGRLGALALAGLAIGGLIVSMVGSGLVFLSYGTTARSARFFGAGDRASAVREGVQ
                     ATWLALALGALFVVVVQAAAVPIVWAIASTGDIAGEALSWLRIAVIAAPAMLMSLAGN
                     GWMRGVQDTVRPLQYVVIGFAVSAVLCPLLVYGWLGLPRMELAGSAVANLVGQWVAAL
                     LFARALLVEHVPLGVNWSVLRAQVVMGRDLFVRTVAFQVCYVSAAAVAARFGAAAVAA
                     HQVVLQLWNFLALVLDSLAIAAQSLVGAALGAGLAAHAKSVAWRVTVFSTLAASVLAV
                     VVALGASVLPELFTNDRSVLDAVEVPWWFLVAQLPVAGVVFGLDGVLLGAGDAVFMRN
                     ATVISALVGFLPLIWLSLVFGWGLAGIWSGLSAFIVLRLVFVGWRAFSGRWAVTGTG"
     gene            9102..>11004
                     /locus_tag="VF874_07795"
     CDS             9102..>11004
                     /locus_tag="VF874_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9175987.1"
                     /translation="MTGTELFCPSCGAQSAPTAKFCSECGKRLTQATPSAEYKQVTVL
                     FADVVHSMELAAAVGPERLREIMADLADLCAAVVQRYGGTVDKFTGDGIMAVFGAPVA
                     LEDHAVRACLAALGVQEEARRLATDVRECDGVDLQLRVGLNSGQVIAGEIGSGRFGYT
                     AIGEQVGMAQRMESVAPPGGVMLSESTARLVENAALLGEPEVIPIKGSDLAVPARRLL
                     AARQRGGPAPWQPTLVGRDLEANTIAGLLDRSITGRGCVVGVVGPAGIGKTRLVGEAM
                     TLAKSLDAEVFSISCESHAKDIPFHAVTRLLRAVARVSGLDDETARAQVRARIPDADE
                     QDLLLLDDLLGIADPEVELPKIDPDARRRRLTALINATQLARTDPSVLVIEDAHWIDD
                     VSESMLIDFLAVIPQTRSMVLITYRPEYRGALAQIPGTQTIALAPLTDSQTSTVIGEL
                     LGADPSVSGVTALIAAQVAGNPFFAEEIVRDLADRGVLEGERGRYVCRTEVADVTVPA
                     TLQAVIAARIDRLQPAGKRTLNAASLIGSHFMTDLFPNLGVDPVFDELIKAELIDQVK
                     FTPRAEYAFRHPLIRTVAYASQLKADRAELAASNACAAGSRPRRRRGLPRAQGALPRH
                     GDVARFRGAY"
BASE COUNT         1868 a         3811 c         3591 g         1734 t
ORIGIN      
        1 gcaaaatccg tgacactaga gggtcgccgg tcgcagcacc accaccgact tcgtgcgcgc
       61 accgtcgtcg cgtagtaacg cgacgacgcg tccgtcgggt gctgtcgcgg cgtacacacc
      121 gtcgataccg gccggcgcca atgcccttcc gtggcgtgcg aattcggcct cgtcgggatt
      181 gaggtctcgg cgcgggaagg tcatcaagca cgcctcgtcc aacgagtagg acaggcaagg
      241 gtgatcggcg aggtcgtcca gggtgtgcgc ctggtcgagg ccgaaccggc cgacccgggt
      301 gcggcgcagc gcggtcaggt gcccgccgac acccagcgcc gcgccgacgt cgcgggccag
      361 tgcgcggatg tatgtgcccg acgagcagtc cacctcgacg tccacgtcga cgacaccctg
      421 ctggcgccgg attgtcagcg cgtcgaaccg gtcgatgcgc acggtgcgcg cggccagctc
      481 gacgggctga ccctcgcgga ccaaccggta ggcccgcttg ccgccgacct tgaccgcgct
      541 caccgccgac gggacctgac gaatttcgcc ccgcagcgcg gcgattgcgg cgttgatcgc
      601 ggcgtcggtc acctcgtcgg cggctgcggt gcgcacgaca tcaccgtcgg cgtcatcggt
      661 cgtggtggcc tgcccgaggc ggatcgttgc ggcgtacgac ttcgtcgacg ccgtcaacag
      721 accgaggatc ttcgtcgcgc gttcgacacc gatcaccagt acgcccgtgg ccatcggatc
      781 gagcgtgccg gcgtggccga ccttgcgagt cgcgaagatt cgacggcagc ggccgacgac
      841 gtcgtggctt gtcaagccgg gcggtttgtc gacgacgacc aggccactca caacacgatc
      901 gctgtcagcg ccagccgctc acgcaccgac caccgaccgc gcaacgccgt cagcggcggt
      961 cctgacaatg cggtcgggtc gatcaggatc tgtgacacga actcgccgct gatcccgtcg
     1021 gagttgacgt cgaaaacgat gtgggcgtcc tcgaagccca gccagcgccg cgtcagcgga
     1081 taccacgcct tgtacgttgc ttccttggcg cagaacagga ttcgatccca gtgcaactca
     1141 tccggcatgg ccgccaattc atgccgctcc gccggaaggc tcaccgcgtc gagcactccg
     1201 ttcggaagca caccgtgtgg ctcggcgtct atgcccaccg accgcacctc cgaagcccgg
     1261 cccaccaccg ccgctcggaa gccctcgcag tgcgtgaggc tgcccaccac tccgtccggc
     1321 cagcacggtt cgcccttgtc ccccttcaag atcggcgccg gcggcacccc gagctcccct
     1381 agcgccaacc gggcgcagtg ccggacggtg acgaactcgt tgcggcgctt tgcaaccgac
     1441 cgctcgatca gcggctcttc gtcgggcagc ggcttgaggt caggcgggtc cgcgtacatc
     1501 tcggcggcgg ccaccgagtc ggacaccacg gtcgagatga gcatcatcta ctggctcgtt
     1561 gctggattcg ctcgcgcacg cgttccgcgt tggcccgcat ctcaggtgtg atcacgaagt
     1621 ggccgccgaa ctcgttgagg tagcccggcg catactccgg gtcgggcagc acctgccgca
     1681 gccaccgata cggcttgcgg cgccgccact ctcgcggata gcccaccgac acctcttcga
     1741 agcggacgcc gtcataccac gtggtccggg gaatgtgcag atgcccgtac accgagcaca
     1801 ccgcgttgta gcgggtgtgc cagtccgcgg tcgccgtcgt gccgcaccag agcgagaact
     1861 cgggatagaa cagcgcctcg cacggctcgc gaaccagcgg gaagtgattc accaacactg
     1921 ttggcgtcat ccagtccagt tgctcgagcc gctcgcgggt gtaggcgacc cgctcccggc
     1981 accatgcgtc gcgggtggcg tacggctccg aggacagcac aaactcatcg gtggccatca
     2041 cgttgcgatc ccgcgcgatc gccagcccct ccgacttcga cgacgcgccc gcgggcagga
     2101 atgtgtagtc gtacagcaga aacatcggca cgatcgtcgc ggggccgccc tgctccgtcc
     2161 acaccggaaa cgggtgctcc ggggtgacga tccccatcgc atcgcacatg tcgaccagat
     2221 agtcgtagcg ggcgcggccg aatacctgaa cggggtcctt gcccgtcgtc cacagctcgt
     2281 ggttcccggg cacccagatc accttggcga accgccgccg cagcaggtcc agcgaccatc
     2341 ggatgtcgtc ggtgcgttcg gcgacgtcgc cggcgacgat cagccagtcg tccggcgacg
     2401 acgggtacag cgactcggtg accggcttgt tgccggtatg acccgtgtga atgtcactga
     2461 cagcccagag cgtcgccaca acagaccaga ttactgaaga cgcggcacac caccgcccag
     2521 cgaactagaa caggttctca tttaccgtcg tgcggacggt gacgcaccgc taaactcccc
     2581 ggcaggggtg cagcgatgag cgcttgcgcg aagagcaata cggcccagca gagggggtgc
     2641 catggccacc aggttgcggc tgttcgccgt gctgtgtgcc ctggtggccg ccgtggtcgt
     2701 ggtggtgcag tccgcgccag cgaacctcgc cgcaacccgc aacgtgcagc tgcggtccac
     2761 cgccgttccg atggacagcc cgaccaccac gatcaagtgg ccggtgatcg acatggtcga
     2821 cccgagcccg ttcgtcccgt gcgaggacat cccgctcgac gtcatcgagc ggctcggcct
     2881 ggcgttcacc ccgcccgaac ccgaagacgg cctgcgctgc cactacgacg cggccaacta
     2941 ccagatggcg atcgagccga tcatctggcg cacgtatcag cagagcctct cccccgacgc
     3001 agtcgagacc accgtcaacg gccaccgcgc cgcctcgttc tgggtgatga agcccaccga
     3061 ctggaacaac aagtggtggt tctcctgcat gctcatgttc gaaaccagct acggcgtgat
     3121 ccaacagtcg ctgttcttct cgccggtcta ctcggatccc gacgtagact gcccgagcga
     3181 gaacatggca cgagcccagc agctcgtccc ctactacaag ttctgacgtt gaccactacc
     3241 acgcagcggc ccgtcgggcc gcgaattcgg cgcatggcca ggcgttccgg ccatcgagcc
     3301 atggcgcgtc ttttcgggct gcccgcgccc acgacccact acacggtgga gcgcggtctg
     3361 cggatcccga tgcgcgacgg ggtcgacctg gtggccgacc actatgcgcc caccgacgcc
     3421 actaagggca cgctgctggt ccgcgtgccc tacggccggg gcctgccgtt atcgctgctc
     3481 tacgcccggc tgtacgccgc ccgcggctac cacgtcgtga tgcagagcgt gcgcggcacc
     3541 tccggctcgg gtggggtgtt cgagccgttt atctatgaag catccgacgg tgccgacacc
     3601 gtcgagtggc tccgccggca gccgtggttc accgggcgat tcgcgacgat cggctcgtcg
     3661 tacctgggaa ccgcccagtg ggcgctgctg acggacccac ctccggagct cgccgcagcc
     3721 gtcgtcaccg ctgcaccaca cgacttgcgc acctcgatgt ggggcaccgg cgcctttcgc
     3781 ctgaccgacg tgctcggctg gtcggacgtg gtgtctcatc aggaacacgg cagtcggctc
     3841 cgcgggatga tctgggctgc cggagcacgc cggcgactga cgcgggcgat ggccgcggtg
     3901 ccgttgggtg agtcggcccg ctcattgctg gccgacggcg ccgcatggtt cgaatcgtgg
     3961 ctggagcacc acgaccccga ggccccgttc tgggagccgt accggttcgg cgccgccctg
     4021 acacaggtga cggttccggt gctgttgatc ggcggctggc aggacagctt cctcagccag
     4081 acactcgagc agtatcaggt cttgcgcggc cgcggcgtcg acgtcgggtt gaccgtgggc
     4141 ccgtggacgc acgcgcagtt gacagcgacg ggcgccgggc gagtggcccg cgaatcactg
     4201 gaatggctcg accgacactt ggccgatgct cctaccggca tggcccggag tccagtgcgc
     4261 gtgtacatca ccggcgacga ttgggtcgag ctgccggatt ggccgccgcc gacgtctccg
     4321 cgagtgctct ttctggcgcc gggtgcggcg ttgagcgacc gcccgccacc cgacaccggt
     4381 ggctcgtcga cgttcaccta tgaccctgca gatccgactc cgatcctcgg tggaccgctg
     4441 gtgctggccg cgaagggcgg atatcgcgag gacagtaggt tggctcgacg cgcggatgtg
     4501 ctgagcttca cgtcagaccc gctgccgtcg gacctctacg tcgtcggaca ccccgtggtc
     4561 gagttgtcgc acgccgccga caccccgcac gtcgacgtcg cggtgcgggt gagcgaggtt
     4621 gactcgcggg ggcgttcgcg caacgtcagc gacggctacc ggcgcgtgac cacatcgtcg
     4681 gaaccgggcg gtgtccacgt cgaactcgag gcggtggccc accgcttccg ggcgggttcg
     4741 agaatccggc tgctcgtcgc cggcggcgcc cacccgcggt tcgaccgcaa cctcggcacc
     4801 ggcgaaccga cgctcaccgc gcagcggctg gtccccgcca cccacaccgt gcatcacggc
     4861 gccgccggag cctcgcggct cattcttccg tcgacgcggc agcgaccgtc agcccacggc
     4921 gcgccggacc cggtcggcga ttccgcgtaa cgtcgcgtcg tcgtgcaccg gcgggcccca
     4981 gtccggggcg accgggacgt cgacccagct ggtgcagccc gcgtagtccg atgtgcgggc
     5041 cagtcgcact ggttcggtga gcgcgcacgc ctggaccacc agcacggtca gccggtgctt
     5101 cggacggaaa tccaggcggt cctcgcgcac cgactcctgc gtccagatgt gcagcggctc
     5161 aatgtcggtc aacccatcgg gattcgaaac tgacacggca gcaacgactt tcgcgccagc
     5221 acgcaacatg atgtgatcgt cgtcgctgtc ggcggcggcc ggcgccagca gatcgcggtg
     5281 ttcttcccgc acgcgttcgg catggctgtg cgcgaccgtc gggaacagca ggaaacgctg
     5341 cgcggcgacc tcgaagcgct tctcgccgat gccgcccttg cgcagcagca cggtctgccg
     5401 cccgtcgagc agcgcgtgca ccgccgcgct ccattccttg agcgcaggcc tgctcgaggt
     5461 ggctgtcatg ccctcagccg ggcgcgtact tccggtcggc gcagcggcgg aatggacttc
     5521 ggcggctgct tacgcggcgg caattcggac agcaggcgtg acgtggtggc agtgacctcg
     5581 gcgacggccg cctcgaacgc gtcgacgttg gccgccgacg gacgcgtgat cccgctgacc
     5641 ttgcgcacgt actggcgggc ggcggcctcg atctcctcgt cggtggcggc aggctgcagc
     5701 ccgcgaagct cggtgatgtt tcggcacatg ccccgacgat aggctcacct cgtggctgac
     5761 gacgatgtgc tgctgatcga aaccgccgac cgggtccgga ctctcacgct caaccgcccc
     5821 cacgtgcgca atgcactgtc gacgaagttg caagacacgc tgttcgatgc gttagcccgg
     5881 gcggagaccg acgacgacgt cgacgtggtg atcctgaccg gcacagatcc cgcattcagc
     5941 gcgggtgtgg acctgaagga gatcagcgcc ggcctgcatc ggcccaaggg ccgcaccggc
     6001 gaggacctgt ccacggttcg ctggccgtcg atgaccaagc cggtgatcgg tgcgatcaac
     6061 ggtgtgtgcg tcaccggcgg cttcgaattg gcgctgagct gcgacatcct gatcgcctcg
     6121 gagaacgccc ggttcgccga cacccacgct cgggtgggct tgttgccagg ctggggtttg
     6181 tcggtgcggc tgccgcaggt ggttgggcgc ggcatggcgt accgcatgag cctgaccggc
     6241 gactacctcg gcgcctacga tgcgctgcgc gtcgggctgg tcaccgaggt ggtgctgcac
     6301 gacgaactga tggacaccgc acgggcggtg gcggcgtcga tcgtcaacaa caaccgcgcg
     6361 gcggtgcaag cgctcctggc gtcgtatcac cgtatcgacg aggcgcagac gagggaggcc
     6421 ttcgcgatcg agcacgagtc cgcggagaac tggctgcgca cgaacgccag cagcgccgac
     6481 atcgccgcga accgcgaggc cgtcatcaag cgcggccgct cccaagtgcg ttgacggcgt
     6541 aggcttcccg agtgaacgag gcacatcagt tctgcggcag cgacgagtgg cggcagatga
     6601 ttcgcgacgt gattttgcct tgggcgctcg acggcaccga cctcggcgac gaggttctcg
     6661 aagtcggccc cggttacggc gccacgaccg atgtgctcag ctgcgcggtg cctaagctca
     6721 cgtcggtcga aatcgacgac gagctcgccg cgatgctgac cgaccggttc gcgggcagtc
     6781 ccgtcgtgag catcgtccac ggcgacgcgt gcgacctcga ctacgaggtc ggtcacttct
     6841 cgggcgcggc ctgcttcacg atgctgcacc acgtcgctcc cgccgaacga caggaccggc
     6901 tgttcggcga gatcgcacgg gttctgcggc caggggcccc gctggtcgcc agcgacagtc
     6961 tcgccagcga cgaactcgcg gccgctcacg atggcgacac ctacaacccg gtcgacccgg
     7021 cgtccttggc cacacggctg gagagggccg ggttcacacg cgtcgccgtg cggaccaacg
     7081 agtacggctg ggcagcggtc gcggtccgag ccccgacggg ctaaccggtt ccggtcactg
     7141 cccatcggcc ggagaacgcc cgccagccga cgaacaccag ccgcagcacg atgaaggcgg
     7201 acagccccga ccagatgccg gccaggcccc agccgaagac cagcgacagc cagatcagcg
     7261 gcaggaaccc caccaacgcg ctgatcacgg tcgcgttgcg catgaacacc gcatcgccgg
     7321 cgccgagcag cacgccgtcg aggccgaaca ccaccccagc gaccggcaac tgcgcaacca
     7381 gaaaccacca cggcacctcg acggcgtcga gcacggaccg gtcgttcgtg aacagctccg
     7441 gcaacaccga ggcgccgagc gcgaccacca ccgccagcac cgacgcagcg agcgtcgaaa
     7501 acaccgtcac ccgccacgct accgacttgg cgtgcgccgc cagccccgcg cccagcgcgg
     7561 caccgaccag cgactgagcc gcgatggcga gcgaatccag caccagcgca aggaaattcc
     7621 acagttgcag taccacttgg tgtgcggcca ccgccgccgc cccgaaacgg gccgcgaccg
     7681 ccgcggcaga tacgtagcac acctgaaatg ccacagtgcg cacgaacagg tcccgaccca
     7741 tgaccacctg tgcgcgcaac accgaccagt tgacgcccag cggcacgtgt tcgacgagca
     7801 gcgcccgagc gaacagcagc gccgcgaccc actgcccgac caggttcgcg acggccgagc
     7861 cggccaactc catgcgcggc agcccgagcc acccatagac cagcagcggg cacagcaccg
     7921 ccgacaccgc gaagccgatg accacatact gcagtggccg cacggtgtcc tgcacaccac
     7981 gcatccagcc gttgcccgcc agcgacatca gcatcgcggg ggcggcgatc accgcgatcc
     8041 gcagccacga caacgcctct ccggcgatgt cgcccgtcga ggcgatcgcc cacacgatcg
     8101 gcaccgcggc cgcctgcacc acgacgacga acaacgcacc gagagccaac gccagccagg
     8161 tggcctgcac accttcgcgc accgccgacg cccgatcgcc cgccccgaaa aaccgtgccg
     8221 atcgtgccgt cgtgccgtac gacagaaaca ccaggcccga cccgaccatg ctgacgatca
     8281 ggccaccgat cgccaggccg gccaacgcca gggcgccgag acgtccgacg acagccgtgt
     8341 cgaagagcag atacagcggt tcggcagcca agaccaccag cgccggcaaa gccagcgcgg
     8401 cgatgcgccg ggtgcttgcc gtcgagtccc ccgaggcagt caattccact cccttaccga
     8461 gccgccgcat cgagatttct tgtattcccc gaggattctg acgcataagg gctgtcgcgg
     8521 tctcctccaa ggcaatctct gccgcacagg tcctatcgga gacgaagtct agaatcggtg
     8581 gcggcggtcg ggtgccaccc agcaccgata aggagccagc atgaaacgac tactcgttgg
     8641 gctaacgacg actgtgctgc tgtcgggagg cgtcgcgggg gtctcggacg caggtaccgc
     8701 tcaagcgcta cccggtcctg cgccgctgaa tagctggccc ggctgtccgg aagacagccc
     8761 gtccggccca tgtcactggt gtccaggaga tcccccggtg cagaccggca atcttcgtgt
     8821 gaacccagtg atttgggacc cgaatgtctg ccacacctac tattacgtct atttcgggca
     8881 gggcaatgtc gcccagaata tttgggaggg agagaccccg ccgccgccgc ctccacctcc
     8941 gccgccaggc cttaatttct gcccaattcc cccgtggtgt ccgtgatcta gtccggggtc
     9001 tcgaaacttc gtgtcgcaga tggcacagcg atatgtcgga catcgttgac cggcggtgaa
     9061 acgatactgg cgtggggccc agcggggatg gtggcggtgg actgacaggg acggagctgt
     9121 tctgtccttc ctgcggcgcg caatctgccc ctacagccaa gttctgtagc gagtgcggta
     9181 agcgactcac gcaagccacc ccatcggctg agtacaagca ggtgacggtc ctgttcgccg
     9241 atgtcgttca ttcgatggag ctcgcggcag cggtgggtcc ggagcggctg cgcgagatca
     9301 tggcggacct cgccgacctc tgcgctgcag tggtgcagcg gtacggcggc acggtggaca
     9361 agttcaccgg agacggcatc atggcggtgt tcggtgcgcc ggtggcgctg gaggaccacg
     9421 ccgttcgcgc ctgcctggcg gccttgggag ttcaggagga agctaggcgg cttgcaaccg
     9481 acgtccgtga gtgcgacggt gtggacctcc agttgcgggt gggtcttaat tccggtcagg
     9541 tgatagccgg tgaaattggt tctgggcgtt tcggttacac cgcgatcggt gagcaagttg
     9601 ggatggctca gcggatggaa tcggtcgcgc cgcccggtgg cgtgatgctc agcgagtcaa
     9661 cagcgcgcct ggttgagaac gctgccttgc tgggcgaacc cgaggtgatc cctatcaaag
     9721 gttctgacct cgcggtgcca gcgcgtcgac tgctcgcggc caggcagcgc ggtggacctg
     9781 ctccctggca gccaaccttg gtgggccgcg acctggaggc caataccatc gccgggctat
     9841 tggatcggtc gatcactggc cgcggatgtg tggtcggtgt cgtgggacca gcgggcatcg
     9901 gtaagacccg tctcgtcggt gaggcgatga cgcttgcgaa aagccttgac gccgaggtgt
     9961 tctcaatctc gtgcgaatcg cacgcgaagg acatcccgtt ccatgcggta acgcggctgc
    10021 tgcgcgctgt cgcccgtgtc agcggtctgg acgatgagac cgcccgcgcg caggtgcgcg
    10081 cgcgtattcc ggatgctgat gagcaggacc tgctgctgct cgatgacttg ctgggcatcg
    10141 ccgaccccga agtagaactg ccgaagatcg atcccgatgc ccggcggcgc cggttaacgg
    10201 cgttaatcaa cgcaacccag ttggcccgga ccgatccgtc ggtcctggtc atcgaggatg
    10261 cgcattggat cgacgacgtt agcgaatcca tgctgatcga cttccttgcc gtgatcccac
    10321 agacccggtc catggtgttg atcacctatc gtccggagta ccgcggagcg ctggcacaga
    10381 tccccggtac gcagacgatt gcgcttgccc cactgacgga ttcgcaaacc tcgacggtga
    10441 tcggcgagtt gctgggcgcc gatccgtcag taagcggcgt caccgcgctc attgcagccc
    10501 aagtcgccgg gaacccgttc ttcgccgaag agatcgtgcg cgatcttgca gaccggggag
    10561 tcctggaagg ggaacgcggc agatacgtct gcagaaccga ggtagccgat gtcaccgtgc
    10621 cggccaccct gcaggccgtg atcgccgcac gcatcgatcg cctccaaccc gcaggcaagc
    10681 ggacattgaa cgcggcctcg ctgatcgggt cgcacttcat gaccgacttg ttcccgaatc
    10741 taggtgttga cccggttttc gacgagttga tcaaggccga gctgatcgat caggtgaagt
    10801 tcacccctcg cgccgagtac gcgtttcgcc acccattaat ccgcacggtg gcctatgcgt
    10861 cgcagctcaa ggccgatcgc gcagagctgg ctgcttcgaa tgcgtgcgct gctggctcgc
    10921 gcccacggcg acgacgaggc ctaccgcgag ctcaaggagc gctaccgcga catggcgacg
    10981 tcgctcgatt tcgaggggca tatg
//
LOCUS       DASWBF010000221         7159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_349345,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000221 DASWBF010000000
VERSION     DASWBF010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349345"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..490)
                     /locus_tag="VF874_07800"
     CDS             complement(113..490)
                     /locus_tag="VF874_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HEX9175988.1"
                     /translation="MTQPDPWKSFRGVMAGILLLEAVVVLLALPVVLVVGHELPQATA
                     TYLITVAALLVVLPAFQRHSWAIWVNLAVQVVLLAGFFVDPAIGFIGVLFTGVWLLIA
                     YLRAEVLRRQKRGLLPGQQPPPE"
     gene            complement(487..1890)
                     /locus_tag="VF874_07805"
     CDS             complement(487..1890)
                     /locus_tag="VF874_07805"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX9175989.1"
                     /translation="MNVAPTPDEIASLLQVEHLLDQRWPETKIDPSLTRISALMDLLG
                     SPQRSYPSVHIAGTNGKTSVARMVDALLTALNQRTGRTTSPHLQSAVERIAIDGEPIT
                     PARYVEVYREIEPFVQMVDKQSQAAGGPAMSKFEVVTAMAFGAFADAPVDVAVVEVGL
                     GGRWDATNVVNAPVAVITPISVDHTNYLGHDIGDIANEKAGIITRQPESLDPTDTVAV
                     IGRQPPAAMEVLLAQVVRADAAVAREDSEFAVLDRQIAVGGQVVTLQGLGGVYSDMFL
                     PLHGEHQAHNAAIALAAVEAFFGAGVQRQLDVDAVRAGFAAVTSPGRLERVRSAPTVL
                     LDAAHNPAGAAALATTLAEEFDFRYLVGVVAVMADKDVAGILAALEPRLDQIVVTHNG
                     SPRGLDVDMLTDAAVERFGTERVVRAATLPDAIETATALVEDAGADDGFSGAGIVITG
                     SVVTAGAARTLFGKDPA"
     gene            complement(1981..4632)
                     /locus_tag="VF874_07810"
     CDS             complement(1981..4632)
                     /locus_tag="VF874_07810"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9175990.1"
                     /translation="MTARPTSSAADALPKAWDPATAESELYRSWVEAGYFTADADSSK
                     PPYSIVLPPPNVTGSLHMGHALDHTLMDALTRRKRMQGYEVLWLPGMDHAGIATQTVV
                     EKQLAVDGKTKEDFGRELFIDKVWDWKHDSGGTIGWQMRALGDGVDWTRDRFTMDEGL
                     SRAVRTIFKRLYDAGLIYRAERLVNWSPVLQTAISDLEVKYEDIEGELVSFRYGSTDD
                     DEPHIVVATTRVETMLGDTAIAVHPDDERYRHLVGTTLPHPFLERKLVVVADEHVDPE
                     FGTGAVKVTPAHDPNDFDIGVRHGLPMPTIMDTRGRIADTGTTFDGMDRFEARVKVRE
                     ALAEQGRIVDEKRPYVHSVGHSERSGEPIEPRLSLQWWVKVDALAKAAGDAVRDGDVV
                     IHPPSLEPRFFGWIDNMHDWCISRQLWWGHRIPIWHAPDGQMVCVGTDETPPAGWEQD
                     PDVLDTWFSSALWPFSTMGWPDHTPEIRKFYPTDVLVTGYDILFFWVARMLMFGMFVA
                     NDDALTLYGSRGPQVPFRNVFLHGLIRDEFGRKMSKSRGNGIDPLDWVEKFGADALRF
                     TLARGASPGGDLTIGEDHARASRNFATKLFNATRFALIYGARPAPLPDDAELTDADQW
                     ILGRLEEVRAEVDSAFDGYEFSRACEALYHFAWDEFCDWYVELAKVQLNNGVQHTTAV
                     LAEVLDTLLRLLHPVMPFVTEALWKALTERESLVIAEWPKPSGRQLDAVAAQRISDMQ
                     KLVTEVRRFRSDQGLADRQKVPARMSGIEPADLDGQVPAFRSLAWLTDPDDGFNATAS
                     VEVRLTGGTVAVELDTSGSVDIEAERRRLEKDLAAAQKELAMTSAKLGNEAFLAKAPA
                     DVVEKIRGRQQVAQEEVERITNRLSSL"
     gene            complement(4675..5274)
                     /locus_tag="VF874_07815"
     CDS             complement(4675..5274)
                     /locus_tag="VF874_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEX9175991.1"
                     /translation="MADLDDLINQIPVADIAKKLGANEGEVNTAIKTLVPALVGGIQQ
                     NVQAEDIDSSELESAVAQQGDSGLLDGEVSVDQVDEQQGHREVARIFGGNDTSQVASA
                     LSSAGAGKNDLIKQLLPILAPIVLAYIGKRLMQGGSSQAAPQASQSGGGLGDILGSIL
                     GGGSQKSDNPLGSILGSVLGGDKGGGLGDILGGLIRGQQ"
     gene            complement(5333..6590)
                     /locus_tag="VF874_07820"
                     /pseudo
     CDS             complement(5333..6590)
                     /locus_tag="VF874_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725990.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trans-acting enoyl reductase family protein"
     gene            6614..>7159
                     /locus_tag="VF874_07825"
     CDS             6614..>7159
                     /locus_tag="VF874_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9175992.1"
                     /translation="MPTRIDVVGAGIAGLAAAVAMQRHGFDVTVFEEKTETSSGAGIS
                     IWPNALAALDEIGLGDAVRDAGGRVTAGAMRWRNGTWLRRPHPDRVVKALGEPLVVIQ
                     RATLTELLQSALRPGTLRHGLAVTGVAQTADGVRLQLSDGSVTQSDAVIGADGTRSLL
                     ARHLNGPLAHTYTGYTAWRGVA"
BASE COUNT         1136 a         2450 c         2369 g         1204 t
ORIGIN      
        1 gccttgcagc gacaatttca ccatttcgca acagggcaca gggctactcc gaggcgggca
       61 caaagcactc ccccagccgg ggtgactcct caggcggctg tgacttaccc ggctactcag
      121 gcggaggctg ctggcctggt agcaggccac gtttctggcg tcgcagcact tcagcacgca
      181 gataggcgat gagcagccac accccggtga acagcacgcc gatgaacccg atcgcggggt
      241 ccacgaagaa gccggcgagc agcacgacct gaacggccag gttgacccag atcgcccacg
      301 aatggcgctg gaacgccgga agtaccacca gcagcgcggc cacagtgatc aggtacgtgg
      361 cggtggcctg cggaagctcg tgcccgacga ccaggacgac cggaagagcc agcagcacca
      421 cgacagcctc cagcagcagg atgccggcca tcacgccgcg gaagctcttc catgggtcgg
      481 gttgggtcat gccggatcct ttccgaacag cgtcctggcg gcgcccgcgg tgaccaccga
      541 cccggtgatc acgatgcccg cgcccgaaaa gccgtcgtcc gcaccggcgt cctcgaccag
      601 cgcggtggcg gtctcgatcg cgtccggcag cgttgctgcg cgaaccacgc gctcggtccc
      661 gaaccgctcg acggcggcgt cggtcaacat gtccacgtca agacctcgcg gcgagccgtt
      721 gtgggtgacg acgatctggt cgagccgcgg ttccagcgcc gcgaggattc cggcgacgtc
      781 cttgtcggcc atcaccgcca ccacaccgac gagataccgg aagtcgaact cctctgccag
      841 ggtcgtggcc agtgcggcgg cacccgcggg attgtgggcg gcatccaaca gcacggtcgg
      901 cgcggagcgc acgcgttcga gccggccggg gctggtgacc gcggcgaacc cggcgcgtac
      961 cgcatcgacg tcgagttggc gctgcacgcc cgcgccgaag aacgcctcca ccgcagcgag
     1021 cgcgatcgcc gcgttgtggg cctggtgctc gccatgcaat ggcaggaaca tgtcggagta
     1081 cacgccgccg aggccctgca gggtgacgac ctggccgcct accgcgatct gccggtccag
     1141 caccgcgaac tccgaatcct cccgtgccac agcggcatcc gcgcggacaa cctgggcgag
     1201 cagcacctcc atcgccgcgg gaggttgccg tccgatgacc gcgacggtgt cggtcgggtc
     1261 gagagattcg ggctgccggg tgatgatgcc cgccttctcg ttcgcgatgt cgccgatgtc
     1321 gtggccgagg tagttggtgt ggtcgacact gatcggggtg atgaccgcga ccggcgcgtt
     1381 gacgacgttg gtcgcgtccc agcgaccgcc gaggcccact tcgacgaccg cgacgtcgac
     1441 gggagcatcg gcgaacgccc cgaacgccat cgccgtgacg acttcgaact tgctcatcgc
     1501 cggaccgcct gcggcctgcg actgcttgtc caccatctgc acgaacggtt cgatctcgcg
     1561 gtacacctcc acgtagcgcg ccggggtgat cggctcgccg tcgatcgcaa tgcgctccac
     1621 cgccgactgc aggtgcgggc tggtggtgcg gccggtgcgc tggttcagcg cggtcaacag
     1681 cgcgtcgacc attcgggcga cggacgtctt gccgttcgtg cccgcgatgt gaaccgacgg
     1741 gtagctgcgc tgcggcgagc ccagcaggtc catcagcgcg ctgatgcgcg taaggctggg
     1801 gtcgatcttg gtttccggcc agcgctgatc cagcagatgc tccacctgca aaagcgacgc
     1861 gatctcgtcg ggagtgggcg cgacgttcac aggccaccgt ctcctcgccg aaactgcatt
     1921 ttcacagcgg aagtccgagt acgggcctgc cggaatacgc agtttcggcg ctgaggctca
     1981 tcacaggctg ctgaggcgat tcgtgatccg ctcgacctct tcttgcgcga cctgctgacg
     2041 tccgcggatc ttctcgacga cgtcggcggg cgccttggcc agaaacgcct cgttgccgag
     2101 cttggccgag gtcatggcga gctctttctg cgcggcggcc aaatcctttt ccagtcttcg
     2161 ccgttcggcc tcgatgtcga cgcttcccga ggtgtcgagc tcgacggcca cggtcccgcc
     2221 ggtgagtcgc acctccaccg acgcggtcgc gttgaagccg tcgtcgggat cggtgagcca
     2281 tgccagtgac ctgaacgcag gaacctggcc gtcgagatcg gcgggctcga ttcccgacat
     2341 gcgcgcaggc actttctgcc ggtcggccag tccctggtcg ctgcggaacc ggcgcacctc
     2401 ggtgaccagc ttctgcatgt cggaaatacg ctgggctgca acagcgtcca actgcctacc
     2461 tgatggcttc ggccactcag cgatgaccag cgactcgcgt tcagtcagcg ccttccatag
     2521 cgcttcggtg acgaacggca tcaccgggtg cagcaaccgc agcaacgtgt cgagcacctc
     2581 ggcaagcacc gcggtggtgt gctgcacgcc gttgttgagt tgtaccttgg ccagctcgac
     2641 gtaccagtcg cagaactcgt cccacgcgaa gtggtacagc gcctcgcagg cgcggctgaa
     2701 ttcgtagccg tcgaacgccg aatctacttc ggcgcgaacc tcttccagcc ggccgaggat
     2761 ccactggtcc gcgtcggtca gctcggcgtc gtccggcagc ggcgctgggc gggcgccgta
     2821 gatcaacgcg aaccgcgtcg cgttgaacag cttcgtggcg aagttgcgcg acgccctggc
     2881 gtggtcctcg ccgatggtca ggtcaccacc ggggctggcg ccgcgggcca gcgtgaaccg
     2941 cagcgcgtcg gcgccgaact tctcgaccca gtccagcgga tcgatgccgt tgccgcgcga
     3001 cttgctcatc ttgcgaccga attcgtcgcg aatcagcccg tgcaggaaca catttcggaa
     3061 cggaacctgc ggtccccgcg acccgtacag cgtgagcgcg tcgtcgttcg cgacgaacat
     3121 gccgaacatc agcatccgcg cgacccagaa gaacaggatg tcgtagccgg tgaccagaac
     3181 gtcggtcggg tagaacttcc gtatttccgg ggtgtggtcc ggccagccca tggtcgaaaa
     3241 cggccacaac gccgacgaga accaggtgtc gagcacgtcg gggtcctgct cccagcccgc
     3301 gggcggggtc tcgtcggtac cgacgcagac catctgtccg tcgggggcgt gccagatcgg
     3361 aatccggtgt ccccaccaca gttgccggga gatgcaccag tcgtgcatgt tgtcgatcca
     3421 gccgaagaag cgcggctcca agctgggcgg atggatcacg acgtcgccgt cgcgcaccgc
     3481 gtcgcccgcg gctttggcca gcgcgtcgac cttcacccac cactgcagcg agagtcgagg
     3541 ctcgatcggc tcgccgctgc gttcggaatg cccgacgctg tgcacgtagg gccgcttctc
     3601 atcgacgatg cggccctgct cggcgagcgc ttcccgcacc ttgactctgg cctcgaaccg
     3661 gtccatcccg tcgaaagtcg tcccggtgtc cgcgattcgg ccgcgggtgt ccatgatcgt
     3721 cggcatcggc agcccatgcc gcaccccgat atcgaagtcg ttgggatcgt gcgcgggcgt
     3781 gactttgact gcgcccgttc cgaattcggg gtcgacgtgc tcgtcggcga cgacgacgag
     3841 cttgcgttcg aggaacgggt gcggcaacgt ggtgccgacc agatggcggt atcgctcgtc
     3901 gtcgggatgc accgcgatcg cggtgtcgcc cagcatcgtc tccacccggg tggtcgcgac
     3961 cactatgtgc ggctcgtcgt cgtcggtcga gccgtaccga aaggagacca gctccccttc
     4021 gatgtcttcg tatttgacct cgagatccga gatcgcggtc tgcagcaccg gcgaccagtt
     4081 gaccagccgc tcggcgcggt agatcagccc ggcgtcatag agccgtttga agatggtccg
     4141 caccgcgcgc gacaaaccct cgtccatcgt gaagcggtcg cgggtccagt cgacgccgtc
     4201 gccgagcgcg cgcatctgcc agccgatggt gccgcccgag tcgtgcttcc agtcccacac
     4261 cttgtcgatg aacagttcgc ggccgaagtc ctctttggtc ttgccgtcga ccgcgagctg
     4321 cttctccacg accgtctgcg tggcgatgcc cgcatggtcc atgccgggca gccacaacac
     4381 ctcgtagccc tgcatgcgct tgcgccgggt cagcgcgtcc atcagcgtgt ggtccagcgc
     4441 gtggcccatg tgcaggctgc cggtcacgtt gggcggcggc aacacaatcg agtacggggg
     4501 cttgctgctg tcggcgtcgg cggtgaagta acccgcttcg acccagcttc ggtacagctc
     4561 gctttcggcc gtcgcgggat cccacgcctt cggcaatgcg tcagctgcgg aggaagtggg
     4621 gcgggcggtc accggtcaat tctaggaatg cgcggtgccc gcccgcgaac gcggctactg
     4681 ctggccgcgg atcagcccgc cgaggatgtc gccgagcccg ccgcccttgt caccaccgag
     4741 gacactgccc aggatgctgc cgagcggatt gtcgcttttc tgcgagccgc caccgaggat
     4801 gctgcccagg atgtcgccga gcccgccgcc ggactgcgac gcctgcgggg cggcttgaga
     4861 gctgccgccc tgcatcagcc tcttaccgat gtacgccaac acgatcggcg ccaaaatcgg
     4921 cagcagctgt ttgatcagat cgttcttgcc tgcacctgca ctcgacaacg ccgacgccac
     4981 ctgcgatgtg tcgttgcctc cgaagatgcg cgcaacctcg cgatggcctt gctgctcatc
     5041 gacctggtcg acgctcactt cgccgtcgag cagaccgctg tcaccttgct gggcgacggc
     5101 agactcgagc tcgctcgaat cgatgtcttc agcctggacg ttctgttgaa tgccgccgac
     5161 aagggcaggc accagggttt tgatggcggt gttcacctcg ccttcattgg cgcccagctt
     5221 ctttgcgata tccgctaccg gaatctggtt gatgaggtca tcgaggtcgg ccatcactgc
     5281 accactttcg ttcctggtcg ttggtccact caatgacgac cctaatccgc tgtcaggtca
     5341 ggcggaccgt atcgagcgac acgcccgctt tcgggaagcg ggcgagcagc gcgtcgccga
     5401 tcgcggcggc aggtgtcagc acgccgcgca gatccgagag ccggtcgcgg tcgagggcca
     5461 gcgccaaacc gctctccccc agcagcacgg aacgtggcct tgtagcccgg gtcgccttgc
     5521 tgggcgatcc tggccacgta gcgcgcgccg gtggtcgtgg tggtgtacgt ctccaccttg
     5581 tagaagccgc gttcgcgcgc cgcctcgctg ggcccgctgc ccggcttcgg cacgatccgc
     5641 tcgacccacc cgcggggcac cagccgaaag aacctggtgc ccaggccgaa ggtcgcagct
     5701 gaggcaccgg tcgcgaccgc ggaaatggcc ggggacagca ccgacgatcc cacgctcatg
     5761 tattccgcat agcggaaccg gcgaccgtag ggccagccca gtagcgcgtt cgtgcgccgg
     5821 acggtccggc tgttgggaat cgccatcgcg aagctggtgg tccaggtgcc ggcgagctcg
     5881 ggcgcgatat cgcggccacg gcgccggccc acgtccggct gagggcccag ttccggttcc
     5941 gcgctgcgat cgggggtcaa cgagtagggg tcctcgagca ggcggcggac gtccggatcg
     6001 ctggacgcgt cgcgcatgag ctccagcatc gaggcggcgg tgccgccgga gaccccgcct
     6061 ctcatccccc gcaagacgaa cgtggtgtcc acgagttcgc cggtgccgtc ctcggcggcc
     6121 ttgcggtaca gcgcgtacac actcaggtcc gacgggatcg agtcgaatcc gcacgagtgc
     6181 acgatgcggg ctccggtgtc gatggcctgc ttgtggtaga ggtcgatgct gctgcggatg
     6241 aagtgcggct cgccggtgag gtccgcgtaa tccgttcccg ccgcagcaca cgcttcgaca
     6301 agtggcagcc cgtatttcgc gtacggtccc accgtggtga caacgacccg ggtccgcgcg
     6361 gccatcgcct ccagcgtcga gggctgcgac gcatcggccg tcaaaagtgg ccagtctttc
     6421 gcgctgtcgc ccagcgtgtc ccgcaccgcg tgcagccgat cggtggaccg gccggccagc
     6481 gcgatccgtg cgccggctcc cgctttggcg aggtattggg cggttagctt gccgacgaaa
     6541 ccggtcgccc cgtacaccac gatgtcgagt tcacgctccg ttgcggtcat gccctgaaac
     6601 tacagtcgtt cccatgccga cacggattga cgtcgtgggt gcgggcattg ccgggttggc
     6661 ggccgcggtc gccatgcagc gtcatggctt cgacgtcacg gtcttcgagg agaagacgga
     6721 gacctcgagc ggcgccggaa tcagcatctg gcccaacgcg ctcgcggcgc tcgacgagat
     6781 cggcctgggc gacgcggtgc gcgacgccgg tggccgcgtc accgcgggag cgatgcgttg
     6841 gcgcaacggg acatggctgc gtcgtccgca ccccgaccgc gtggtgaagg ccctaggtga
     6901 accactggtg gtgatacaac gcgccacgct gaccgagctg ctgcagagcg ccctgagacc
     6961 cggcaccctg cggcatggac tcgcagtgac gggcgtcgct cagaccgccg acggcgtgcg
     7021 gctgcagctg tcggacggca gcgtcaccca gtcggacgcg gtgatcggcg cggacggcac
     7081 ccgctccctg ctcgcccgtc acctcaacgg accgctggcc cacacctaca ccggctatac
     7141 cgcgtggcgc ggcgtggcc
//
LOCUS       DASWBF010000222         8267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1004379,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000222 DASWBF010000000
VERSION     DASWBF010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004379"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VF874_07830"
     CDS             <1..1194
                     /locus_tag="VF874_07830"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX9175993.1"
                     /translation="VELDGGEMTRIMWKFIKETLILPYLDINLEYYDLGIEHRDETDD
                     QVTIDAAHAIQKHGVGVKCATITPDEARVKEFDLKQMWLSPNGTIRNILGGTIFRAPI
                     VINNVPRLVPTWTKPIIIGRHAFGDQYRADNFKVDRAGTFTVTFTPDDGSEPIQHEVV
                     HIPEDGGVVMGMYNFKKSIQDFARSSFKYGLKEKYPVYLSTKNTILKAYDGMFKDEFQ
                     RIFDEEFRNDFETNGLTYEHRLIDDMVASCLKWEGGYVWACKNYDGDVQSDTVAQGYG
                     SLGLMTSVLMTPDGKIVEAEAAHGTVTRHFRQHQAGKPTSTNPIASIFAWTRGLDHRG
                     KLDNTPEVVEFAHKLENVVIDTVEGGQMTKDLALLIGPDHKYLSTEEFMGALDDNLKK
                     ALVDS"
     gene            complement(1258..1578)
                     /locus_tag="VF874_07835"
     CDS             complement(1258..1578)
                     /locus_tag="VF874_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175994.1"
                     /translation="MIVGVTLAVLGNHRGPTSNAPTGPTQTVLPFTGLDGPWGVAVDT
                     AGNVYVADTGNSRVVKLAAGSTTQTVLPFTALSLPGGVAVDNAGSVYVPEDFSNRVVK
                     LAPG"
     gene            complement(1630..2634)
                     /locus_tag="VF874_07840"
     CDS             complement(1630..2634)
                     /locus_tag="VF874_07840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9175995.1"
                     /translation="MAETQFGRYQLLELLGRGGMGEVWRAHDTATDRIVALKLLPTHL
                     SHDEVFQQRFRREAHAAARLNNPHVIPIHFYGEIDGRLFVDMRLIEGRDLATVLDDGP
                     LEPSRAVRIIEQVAKALHAAHKVGLVHRDVKPSNILLDEDDFAYLIDFGIARAANETG
                     LTGTGATIGSWHYMAPERFSARDADARADIYALACVLYECLTGSRPFPGDGLEQQYGG
                     HVATPPPRPSTTQPTVAAAFDEVIATGMAKDPDDRYATTVALARAAQDAITTPIPRPA
                     PAVAPPPPPEPTTVPVVPPTAVGVSASAPIQQRQPHEVPTLRAPDRAPPAPAPSTRPR
                     "
     gene            complement(2778..3623)
                     /locus_tag="VF874_07845"
     CDS             complement(2778..3623)
                     /locus_tag="VF874_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9175996.1"
                     /translation="MRERAPIHLSPPDGSGEPMLLLHPFMMSQNVWKKVAPQIADTGR
                     YEVFAPTMAGHNGGPKGRWKLDSGTLADHVERQIDALGWTTAHIVGNSLGGWVAFELE
                     RRGRARTITAIAPAGGWHQWSPLKFEIVGKFLAGAPLYVLVKLLGPRVLSLPFSRYLA
                     HFPISASPETLPEGDLLDIIDDVQHCPAYYQLLMKSLVAPGLMELADTRIPTHLVICE
                     KDRVLPHPRYTRHFHTHIPDITQITELDGVGHIPMFEAPDRVADVIVNWVDEHAGTPP
                     VRSTG"
     gene            3708..4715
                     /gene="trpS"
                     /locus_tag="VF874_07850"
     CDS             3708..4715
                     /gene="trpS"
                     /locus_tag="VF874_07850"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9175997.1"
                     /translation="MTSQRRVFSGVQPTSDSLHLGNALGAVTQWVALQAEFDAFFCVV
                     DLHAITVPQDPDVLRRRTLLTAAQYLALGIDPARSTVFVQSHVPAHAELAWVLGCFTG
                     FGQASRMTQFKDKAQRQGSEATTVGLFTYPVLQAADVLLYDTDLVPVGEDQRQHLELA
                     RDVAQRFNSRFPGTFVVPEVLVPKATAKIYDLADPTSKMSKSAGTEAGLISLLDDPKM
                     TAKKIRSAVTDSEREVRYDPDEKPGVSNLLTIQSAVTGTEIDKLVEAYAGRGYGDLKA
                     DTADAVVEFVTPLKARVDELVGDPTELEAILTSGAERAREVSAKTIANVYERLGFLQV
                     P"
     gene            4729..5763
                     /gene="yhjD"
                     /locus_tag="VF874_07855"
     CDS             4729..5763
                     /gene="yhjD"
                     /locus_tag="VF874_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HEX9175998.1"
                     /translation="MRAEKDPDKPGLLDRLRARYPWLDHAIRTQERYRDTRGNFFAAG
                     ITYFTIFALFPLLMVAFAIAGFVLASQHELLSQIDDKVKQTVPGDLGKQVVELIDSAI
                     NSRTSVGIIGLVAALWAGLGWMGNLREALTAMWGQPRGERNFVRTRLSDLVALISVFL
                     AIVFTAALTALSGGGLMRTVLGWLGLQDVPGVSVALQIASLFASVLISWLLFTWMIAR
                     LPRESLSLKSAARAGLLAAVGFEVFKQLGAVYLQKIVSGPAGATFGPVLGLLVFAYIT
                     ARLILFATAWAATSTENLAAAPVAPPEPAIIAPRNVDSEGISPKAAFVAGALGALGGL
                     GLSRMLRRRP"
     gene            complement(5835..6170)
                     /locus_tag="VF874_07860"
     CDS             complement(5835..6170)
                     /locus_tag="VF874_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9175999.1"
                     /translation="MRLTFGTAQFLAGVGVAIAVTLAPAAAADTIGSATSTHDDTLHQ
                     ILHTRHTVTPAQPAPAPAPAAPAPAPRQAPSGPVMRPAQPQHDPNEARSIPAISVPVA
                     PSVQPQPAI"
     gene            complement(6323..7159)
                     /locus_tag="VF874_07865"
     CDS             complement(6323..7159)
                     /locus_tag="VF874_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9176000.1"
                     /translation="MANHLFSDDYVAASLTFLAAVAARTSCVRLGTAVVLAPLHNAVE
                     IAEQAAMVDLISGGRLDLGLDTGYRVPEYELFGADLRWRYAQTDAKAKEVRRLLSPGG
                     VTPHPVQAEVPIWMGYQGPKGARRAGPARGRSAVGGRAQLASVRDGLQAGGHDLARAR
                     MTGWINAWVSDDPEADWPTVRVHMAHQFDTYASHGVEGTGQPPPPLVDPERVRAGTTD
                     QLLGYIWCDQPATVAEKISAYTGDAPVETVLIFASLAGMAEPMVIRHIQTIATKLVPL
                     LA"
     gene            complement(7725..8105)
                     /locus_tag="VF874_07870"
     CDS             complement(7725..8105)
                     /locus_tag="VF874_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176001.1"
                     /translation="MVAIDGTVGSWEPHGKGTVDRLHQLAGRGWRPQDCAVLDDYSGQ
                     LQRWTLTAAELLAESPRVFLHVPCPRCGERFAYRRDSAGELVRVRALRVSESGCKCSA
                     CGAFWPPDRFEWLARLLGCPPLTA"
     gene            complement(8148..>8267)
                     /locus_tag="VF874_07875"
     CDS             complement(8148..>8267)
                     /locus_tag="VF874_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176002.1"
                     /translation="GLPGAMNELPVRVGVAIVGAMIVFGLILAARSVNRRTQN"
BASE COUNT         1441 a         2675 c         2777 g         1374 t
ORIGIN      
        1 gtcgaactcg acggcggcga gatgacccgg atcatgtgga agttcattaa ggaaacgctg
       61 atccttcctt acctggacat caacctggag tactacgacc tgggcatcga gcatcgcgac
      121 gagaccgacg accaggtcac catcgatgcc gcgcacgcga tccaaaagca tggcgtcggc
      181 gtgaagtgcg ccaccatcac gccagacgaa gcgcgcgtca aggaattcga cctcaagcaa
      241 atgtggctgt cgcccaacgg caccatccga aacatcctgg gcggcaccat cttccgcgca
      301 ccgatcgtga tcaacaacgt accccgtctg gtgcccacct ggaccaagcc gatcatcatc
      361 ggccggcacg ccttcgggga ccagtaccgc gcagacaatt tcaaggtcga ccgcgccggt
      421 acgttcaccg tgaccttcac acctgacgac ggcagtgagc cgatccagca cgaggtggtc
      481 cacattcccg aggacggcgg cgtggtgatg ggcatgtaca acttcaagaa gtccatccag
      541 gatttcgcac gctcctcgtt caagtacggg ctgaaggaga aataccccgt ctacctgtcg
      601 accaagaaca cgatcctcaa ggcctacgac gggatgttca aggacgagtt ccagcgcatc
      661 ttcgacgagg agttcagaaa cgacttcgag accaacggcc ttacctacga gcaccggctg
      721 atcgacgaca tggtggcgtc ctgcctgaag tgggagggcg gctacgtctg ggcgtgcaag
      781 aactacgacg gcgacgtgca gtccgacacc gtcgcgcagg gctacgggtc gctgggcctg
      841 atgacgtcgg tgctgatgac accggacggc aagatcgtcg aagcggaggc cgcgcacggc
      901 accgtgacgc ggcacttccg tcagcaccag gccggcaagc ccacgtcgac gaacccgatc
      961 gcgtcgatct tcgcctggac ccgtggcctg gatcaccgcg ggaagctcga caacaccccc
     1021 gaggtcgtcg agttcgccca caagctggag aacgtggtca tcgacaccgt cgagggcggg
     1081 cagatgacca aggacctcgc gctgctgatc gggcccgacc acaaatatct gtccaccgag
     1141 gagttcatgg gcgcactcga cgacaacctg aagaaggcgc tggtggattc ctagccggtc
     1201 gtacacctgg gatcggtcca gcgacgcagt cgatcagggt gccagcgcgg tgacacctca
     1261 cccgggcgcc agcttcacca cccggttgct gaagtcttcg gggacgtaga cgctgcccgc
     1321 gttgtccacc gccacacccc ccggaaggct gagggcggtg aagggcagca cggtctgggt
     1381 ggtcgacccg gccgccagct tcaccacccg gctgttgccc gtgtcggcga cgtagacgtt
     1441 gcccgcggtg tccaccgcca caccccacgg accatcgaga ccggtgaagg gcagcacggt
     1501 ctgggtcggc cccgttggcg cgtttgaggt tggcccgcgg tggtttccta agacggcgag
     1561 tgtgacgccg acgatcacgg cgacgacggt tagaacgacg ccggccgata tgaccaccga
     1621 tcggcgacgt caccgcgggc gggtgctcgg tgccggtgca ggcggtgctc gatcgggtgc
     1681 tcgcagggtc ggcacttcgt ggggctggcg ctgctggatg ggcgccgagg cggagacgcc
     1741 gacggcggtc ggcgggacaa ccggaacggt tgtgggctcc gggggcggcg gcggcgcgac
     1801 ggccggcgcg ggccgcggaa tgggcgtcgt gatcgcgtcc tgggcggcgc gcgccaacgc
     1861 gacagtggtg gcataacggt cgtcggggtc tttggccatg cccgtggcga tgacctcatc
     1921 gaacgcggcg gccacggtcg gttgggtggt tgacggccgc ggcggtggag tcgcgacatg
     1981 gccgccgtac tgctgctcca gaccgtcgcc gggaaacggc cggctgccgg tcaaacactc
     2041 atacaacaca caggccagcg cgtagatgtc ggcgcgggcg tcggcgtcgc gcgcgctgaa
     2101 ccgctccggt gccatgtagt gccaactgcc gatcgtcgcg ccggtaccgg tcaaccccgt
     2161 ctcgttggcg gcgcgcgcga tgccgaaatc gatcagatac gcaaagtcgt cctcgtcgag
     2221 caagatgttg gacggcttga catcgcggtg caccagcccg accttgtgcg cggcatgcaa
     2281 cgccttggcc acctgctcga tgatgcggac cgcccgcgac ggctccagcg ggccgtcgtc
     2341 cagcacggtc gccaggtcgc ggccctcgat gagccgcatg tccacaaaca gtcgcccgtc
     2401 gatctcgccg tagaagtgga tcgggatgac gtgcgggttg ttcagtcgcg ccgccgcatg
     2461 ggcttcgcgg cggaagcgct gctgaaacac ctcgtcatgg gaaaggtggg tgggcagcag
     2521 cttgagcgcg acaatccgat cggtggccgt gtcgtgggcc cgccacacct cgcccatccc
     2581 gccacggccc agtagctcga gtaactggta gcggccaaac tgtgtttccg ccacctcgcc
     2641 tcccgcgcac caccggttcg gtattttccg acaactgaca ataatcacca ccggccacgc
     2701 catccgctgc tttgctgaca aatggcgtcc gagaaggacg gtgccaaaca ctccaccgga
     2761 gtggtttgac gcgtgcttta gcccgtcgat cgcacgggcg gcgtgccggc gtgctcgtcg
     2821 acccagttca cgatcacgtc ggcgacgcgg tcgggcgcct cgaacatcgg gatgtggccg
     2881 accccgtcga gctcggtgat ttgggtgatg tcgggaatgt gcgtgtggaa gtgccgggtg
     2941 tagcggggat ggggcagcac ccggtccttc tcgcagatca ccagatgtgt cgggatgcgg
     3001 gtatcggcga gctccatcag ccccggcgcc accagcgact tcatcaacag ctggtagtag
     3061 gccggacagt gctgaacgtc gtcgatgatg tcgagcaggt caccttcggg cagcgtctcc
     3121 ggtgatgcgc tgatcgggaa gtgggccagg tagcgactga acggcagact cagcacgcgg
     3181 gggccgagca gcttgaccag cacgtacaac ggcgcgccgg ccaggaactt gccgacgatc
     3241 tcgaacttca gcggcgacca ctggtgccac ccgccggccg gcgcgatcgc ggtgatggtt
     3301 cgggcccggc cgcgccgttc gagctcgaag gccacccagc cgcccagcga attgccgacg
     3361 atatgcgcgg tggtccaccc gagcgcgtcg atctggcgct cgacgtggtc ggcaagtgtt
     3421 ccgctgtcga gtttccacct gcccttcggc ccgccgttgt ggccggccat ggtcggcgcg
     3481 aagacctcgt agcggccggt gtcggcgatc tgcggcgcga ctttcttcca gacgttctgc
     3541 gacatcatga aggggtgcag cagcagcatc ggctcgccgg agccatctgg agggctcaag
     3601 tgaatgggtg cccgctcgcg catgatcgcg accataaagc cggtaccgcc ggtaccgcaa
     3661 gtaaccagga cggccccgtg cgcccggcat gaaaagatcg acccatcatg accagtcaga
     3721 gacgcgtctt ctccggcgtt cagccgacct ccgattcatt gcacctgggc aatgcgctcg
     3781 gcgcggtgac gcagtgggtg gcgctgcagg ctgagttcga cgcgttcttc tgtgtggtcg
     3841 acctgcacgc gatcaccgtg ccgcaggacc ccgacgtgct gcggcgccgg acgctgttga
     3901 cggccgcgca gtacctggcg ttgggcatcg acccggcccg cagcacggtg ttcgtccaga
     3961 gccatgtccc ggcgcacgcc gaattggctt gggtgctggg ctgtttcacc ggcttcgggc
     4021 aggcctcgcg gatgacgcag ttcaaggaca aggcgcagcg ccaaggcagc gaggcgacca
     4081 ccgtcgggct gttcacctat ccggtgttgc aggccgccga cgtgctgctc tacgacacgg
     4141 acctggtgcc ggtgggtgag gaccagcgcc agcacctcga gttggcccgc gacgtggccc
     4201 agcgcttcaa cagccggttc ccgggcacgt tcgtcgtccc cgaggtgctg gtccccaagg
     4261 cgacggccaa gatctacgac ctcgcggacc cgacgtcgaa gatgagcaag tcggcgggca
     4321 ccgaagccgg gttgatcagc ctgctcgacg acccgaagat gaccgccaag aagatccggt
     4381 ccgcggtcac cgacagcgag cgtgaggtgc gctacgaccc cgacgagaag ccgggggttt
     4441 cgaaccttct gaccatccag tcggcggtca ccggcaccga gatcgacaag ctggtggagg
     4501 cctatgcggg ccgcggctac ggcgacctca aggcggacac tgccgacgcg gtggtcgagt
     4561 tcgtcacacc gctgaaggcg cgcgtggacg agttggtcgg cgacccaacg gagttggagg
     4621 cgattctcac gagcggtgcg gagcgggctc gtgaggtgtc ggcgaagacg atcgcgaacg
     4681 tgtatgagcg gctagggttt ctgcaggttc cataacggga gacagcgcat gcgggccgag
     4741 aaagacccag acaagccggg actcctcgac cgcctgcggg cgcgctatcc gtggttggac
     4801 cacgcgatcc ggacgcagga acgttaccgc gacactaggg gcaacttctt cgccgcggga
     4861 atcacctact tcacgatatt cgcgctgttc ccgttgctga tggtcgcttt cgcgatcgcc
     4921 gggttcgtgc tggcgagcca gcacgaactt ctcagccaga tcgacgacaa ggtcaagcag
     4981 accgttcccg gtgatctcgg caagcaggtg gtcgagctga tcgattccgc gatcaattcc
     5041 agaacgtcgg tcggcatcat cgggcttgtc gccgcgctct gggcggggtt gggctggatg
     5101 gggaacctgc gtgaggcgtt gacggcgatg tgggggcagc cgcgcggcga acgcaacttc
     5161 gtgcgcacca ggctgtccga cctcgtcgcg ttgatctcgg tattcctcgc gatcgtcttc
     5221 accgccgcgc tgaccgctct ttccggcggt ggcctgatgc gaacggtgtt ggggtggttg
     5281 ggtcttcaag acgtaccggg ggtcagcgtg gcactgcaga tcgcatcgtt gttcgcgtcg
     5341 gtgctgattt cctggctgct gttcacctgg atgatcgccc gcttaccgcg tgagtcgctg
     5401 agcttgaaaa gcgccgcacg tgccggcctg ttggcggccg tcggcttcga ggtcttcaag
     5461 cagctgggtg cggtgtatct gcagaagatc gtcagcgggc cagcgggtgc gacatttggg
     5521 cccgttctgg gtctgctggt gttcgcgtac atcaccgcgc ggctgattct attcgcgacc
     5581 gcgtgggccg cgacgtcgac ggaaaacctt gccgcggcac cggttgcgcc cccggagccc
     5641 gcgatcatcg caccgcggaa tgtggactcc gagggcatca gcccgaaggc ggcgttcgtc
     5701 gcgggtgcac tgggagcact tgggggactt ggtctttcac ggatgttgcg ccggcggccc
     5761 tgaccgcgcc gagcgtgagc ccacggcgaa aatccgcgcc cattttcgca accggcgcac
     5821 gctcgacggt gtgatcagat cgcaggctgt ggttgcaccg aaggcgcgac gggcactgat
     5881 atggcgggaa tcgatcgcgc ctcattggga tcatgctggg gttgcgcggg gcgcatcacc
     5941 ggacccgacg gcgcctgccg cggcgcgggt gccggtgctg ctggcgctgg agccggcgcg
     6001 ggttgagccg gcgtgacggt gtgccgagtg tgcaagatct ggtgcagggt gtcgtcgtgt
     6061 gtggaggtcg ccgagccgat cgtgtcggcg gccgccgctg gtgcgagggt cacggcgatc
     6121 gccacgccga cgccggcgag gaactgtgcg gtgccgaatg tcagtcgcat gtacttctat
     6181 ccttccgatg gcgattgcag ccgatttgac tgcgcttacg gaaagagagt gcgcgagaat
     6241 tggcaaatcg ttacaccgga atctcacgtc aattctcgac agcctattca cagtcagttg
     6301 acggaaatgg cgcagtgtcg gattaggcga gcaacggaac caatttggtg gcgatcgtct
     6361 gaatgtgccg gatgaccatc ggctctgcca tgccggccag cgaagcgaag atcaacaccg
     6421 tctccaccgg ggcgtcgccc gtgtaggccg agatcttctc cgccacggtc gcgggttggt
     6481 cgcaccagat gtagccgaga agctggtcgg tggtgccggc gcggacgcgc tccgggtcga
     6541 ccagcggcgg aggcggctgc ccggtgcctt cgacaccgtg actggcgtag gtgtcgaact
     6601 ggtgagccat gtgcacccgc accgtcggcc agtcggcctc cgggtcgtcg ctcacccagg
     6661 cgttgatcca cccggtcatc cgggcacggg cgaggtcatg tccgccggcc tgcagcccgt
     6721 cgcgtacgga ggccagttgc gcgcgtccgc cgacagcact ccggccccga gcaggcccgg
     6781 cccgccgcgc gcccttcggg ccctgataac ccatccagat gggaacctcg gcctgcaccg
     6841 ggtgcggcgt cacaccgccg ggcgacagca ggcgccgaac ctcctttgcc ttggcatcgg
     6901 tttgggcgta gcgccaacga aggtccgcgc cgaacaactc gtactcgggg acccggtatc
     6961 cggtgtccag cccgagatcc agccggccac ccgagatgag gtcgaccatc gcggcctgtt
     7021 cggcgatttc cacggcattg tgcagcggcg ccagcaccac cgcggtgccg aggcgaacgc
     7081 atgaggtgcg ggcggcgacg gcggccagaa acgtcaacga cgccgcgacg tagtcgtcgg
     7141 agaacaggtg attagcgatt gatcaatcgc gggggttctc gctgactgcc gttagtcgct
     7201 gcggcacaac gggtttagtg ctgaaaccgt tgccaaccat tgatggtagt tttcaccgat
     7261 tctcggtcat aaacaatgaa taaacgatga gtggactgac tgcgggccgt cacgccaggc
     7321 agctgctcac agcccagcag ccgcgccaac cactcaaacc gatccggcgg ccaaaacgcg
     7381 cgtgagtcgc gccgactaga ctcctcccca tgtccggccg ggtcagagcg ccccgttatc
     7441 tgatctcagt gttggcgacg gcggcggccg ctgcgggaat cctcgcagcg ccgccagcgg
     7501 cagcagacga gtgcatcaag gacgctgacg ggatcaccgt gtgcgggcat ggtgacgttc
     7561 ggggcagtca gaaatttagt cccgacatcg aaacgggtcc cgtttacccg tccgcgtgcg
     7621 aggaagacga ctggtattgc gacgccggcg ttcctggctt atagagcgga tcggcccgcg
     7681 atagccgttt cctgccccag cggtcgacct cgtcggcgaa aggatcacgc cgtcagcggc
     7741 ggacaaccaa gcagccgcgc cagccactca aaccgatccg gcggccagaa cgcgccacac
     7801 gccgagcatt tacaccccga ctccgacacc cgcagcgccc gcacccgcac cagctcgccg
     7861 gcgctgtcgc ggcggtacgc gaaccgttca ccacaacgcg gacacgggac atgtagaaac
     7921 actctcggac tctcggctag cagttccgcc gccgtcagcg tccagcgctg caactggccg
     7981 ctgtagtcgt cgagcactgc gcagtcctgt gggcgccagc cgcggccggc cagctggtgc
     8041 agccgatcca ccgtgccctt gccgtgcggc tcccacgagc ccaccgtgcc gtcgatcgca
     8101 accaacagcg ccaggacggc cgtgctgcac ggcggccggg gtaaagacta attctgcgtt
     8161 cgccggttga ccgatcgcgc ggcgaggatc aggccgaaca cgatcatcgc gcccacgatc
     8221 gccaccccca cccgcaccgg cagctcgttc atcgcgcccg gcaggcc
//
LOCUS       DASWBF010000223         5631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_680112,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000223 DASWBF010000000
VERSION     DASWBF010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680112"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VF874_07880"
     CDS             <1..402
                     /locus_tag="VF874_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176003.1"
                     /translation="LACCGALIGAAMCAMPGVGAAHAAPGEWAAIAYDPDGRTGWPNP
                     GFAVWVNHAAGQREAVAGSLGQCSSGPGFGGSSAPCELVALVQDGCVALVSLDGVAQP
                     AAAVAPTAQGAVDAAAAGHRVTEVIYNDCTY"
     gene            complement(408..1091)
                     /locus_tag="VF874_07885"
     CDS             complement(408..1091)
                     /locus_tag="VF874_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX9176004.1"
                     /translation="MRAVLWDMDGTLVDSEKHWDVAMHALYHRLDGTLTPGVRVATVG
                     GSAENVMRIVYDDLGLDPDPAQMAATADWLHDYTADLFDRGLPWCEGAPDLLEALAID
                     GVPMALVTNTRRVLTERALSSIGRQYFSAVVCGDEVARGKPAPDPYELAAELLGASPE
                     QCLAIEDSVTGTASAESAGCPVLVVPNDVDVPRGPRRRHVASLAGLDASGLRAIYEQL
                     DSQFGERTA"
     gene            complement(1093..4668)
                     /gene="metH"
                     /locus_tag="VF874_07890"
     CDS             complement(1093..4668)
                     /gene="metH"
                     /locus_tag="VF874_07890"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9176005.1"
                     /translation="MTAASRRDYDTDLLATLSEKVVVGDGAMGTMLQAADLTLDDFNG
                     LEGCNEILNDTRPDVLEHIHRAYFEAGADAVETNTFGCNLSNLGDYDIADRIRELSEK
                     GTATARRVADELSTPEAKRYVLGSMGPGTKLPTLGHTEYAVIRDAYTEAALGMLDGGA
                     DAILVETCQDLLQLKAAVLGSRRAMAQAGRHIPVFAHVTVETTGTMLLGSEIGAALTA
                     VEPLGVDMIGLNCATGPAEMSEHLRHLSRHARIPVSVMPNAGLPVLGANGAEYPLTPE
                     ELAEALSGFITEFGLSLVGGCCGTTPEHIRQVADAVRGVERAARHVTYEPAVSSLYAA
                     TPFAQDASVLMIGERTNANGSRAFRDAMIAEDYERCLDIAKEQTRDGAHLLDLCIDYV
                     GRDGVADMTALASRLATASTLPIMLDSTETPVLQAGLEHLGGRCAINSVNYEDGDGPE
                     SRFHKTMELVAEHGAAVVALTIDEEGQARTADKKVQIAERLIDDITTNWGVDESSILI
                     DCLTFTICTGQEESRRDGIETIEAIRELKKRHPDVQTTLGLSNISFGLNPAARQVLNS
                     VFLHECQEAGLDSAIVHASKILPMNRIPEDRRDVALDLVYDRRRDGYDPLQELMQMFE
                     GVSASSSKESRAAELAALPLLERLARRIVDGERNGLEADLDEAMTQKPPLDIINDDLL
                     SGMKTVGELFGSGQMQLPFVLQSAEVMKTAVAYLEPHMERSDDDAGKGRIVLATVKGD
                     VHDIGKNLVDIILSNNGYEVVNIGIKQPINTILEVAQDKSADVVGMSGLLVKSTVVMK
                     ENLEEMNHRGVADKFPVLLGGAALTRSYVENDLADIYSGDVHYARDAFEGLHLMDQIM
                     NAKRGVAPDTDSPKAVAAREKEAERKARHERSKRIAAERKAKETPVEVPERSDVAADN
                     EVPVPPFWGTRIVKGVALAEYTGLLDERALFLGQWGLRGARGSEGPSYEELVESAGRP
                     RLRYWLDRLSTDGILAHAAVVYGYFPAVSDGDDVIVLTEPRPDAPERFRFTFPRQHRG
                     RFLCIADFIRSREKAAGTGQVDVLPFQLVTMGSPIADFANELFAANSYRDYLEVHGIG
                     VQLTEALAEYWHRRVREELKFSDHAMAAEDPASVEDYFKLGYRGARFSFGYGACPNLE
                     DRTKMMALLEPERIGVTLSEELQLHPEQSTDAFVLHHPEAKYFNV"
     gene            4749..>5631
                     /locus_tag="VF874_07895"
     CDS             4749..>5631
                     /locus_tag="VF874_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9176006.1"
                     /translation="MTPSDATRGFELPVLHDAVVVAAFEGWNDAGDAASDALEHLDAI
                     WEAEPIIEIDDEAYYDYQVNRPVIRQVDGVTRELVWPSMRISHCRPPGSERDIVLMHG
                     VEPNMRWRTFCAELLAIADKLNVATVVILGALLADTPHTRPVPVSGAAYSPESARFFG
                     LEETRYEGPTGIAGVFQDACVNAGIPAVTFWAAVPHYVSQPPNPKATVALLQRVEDVL
                     DIEVPLADLPAQAEEWEAAVTEMTSDDDEIAEYVAQLEERGDAEVDMTEALSKVDGDA
                     LAAEFERYLRRRGPGFRG"
BASE COUNT          891 a         1913 c         1853 g          974 t
ORIGIN      
        1 ctcgcttgct gtggcgcgct gatcggcgcg gcgatgtgcg caatgcccgg tgtcggagcg
       61 gcgcatgccg cgcccggtga gtgggccgcc attgcgtatg accccgacgg ccgtaccggc
      121 tggcccaacc cgggcttcgc agtctgggtg aaccatgctg cgggccagcg ggaagctgtg
      181 gccggcagcc ttggccagtg cagctctggg cctggcttcg gcggcagctc tgcgccctgc
      241 gagctcgtcg cgcttgtgca ggacggctgt gtggccctgg tgtcgctgga tggcgtcgcg
      301 cagcccgccg ccgctgtcgc accgaccgct cagggcgcag tcgacgccgc tgcggcaggg
      361 catcgggtca ccgaggttat ctacaacgac tgcacttact gagcggctca ggcggtacgt
      421 tcgccgaact ggctgtccag ttgctcgtag atcgcccgta gccctgaggc gtcgagaccg
      481 gccagcgagg cgacatgtcg acgccgtgga ccgcgcggca cgtcgacgtc gttgggcacc
      541 accaacaccg ggcatcccgc gctctcagcc gacgccgtgc cggtgaccga gtcctcgatg
      601 gccaggcact gctcggggga tgcgcccaac agctctgccg cgagctcgta gggatccggc
      661 gccggtttgc cacgagccac ctcgtcgccg cacaccaccg ccgaaaagta ctgccggcca
      721 atgctgctca atgcccgctc ggtgaggacc cgacgggtat tagtgacgag cgccatcgga
      781 acaccgtcaa tcgccaacgc ctccagtaaa tccggggcgc cctcgcacca cggcaggccc
      841 cggtcgaaca agtccgcggt gtaatcgtgc agccagtctg cggtggcggc catctgcgcc
      901 gggtcggggt caaggcccag gtcgtcgtaa acgatccgca tgacgttctc cgccgatccg
      961 cccaccgtgg ccacgcgcac cccgggtgtc aacgtaccgt cgaggcggtg gtacagggca
     1021 tgcatcgcga cgtcccagtg cttctcggag tcgaccaggg tgccgtccat gtcccagagc
     1081 acggcacgca tttcagacat tgaaatactt ggcttcggga tggtgcagca cgaacgcgtc
     1141 ggtggactgc tccggatgca actgcagctc ctcggacagc gtgacaccga tgcgctcggg
     1201 ctcgagtagc gccatcatct tggtgcggtc ctccagattg gggcacgctc cgtacccgaa
     1261 cgagaaccgt gcaccgcggt agccgagctt gaagtagtcc tccaccgatg ccggatcctc
     1321 cgctgccatc gcgtggtcgg agaatttcag ctcttcccgg acgcggcggt gccagtactc
     1381 ggccagcgct tccgtcagct gcacgccgat gccatgcacc tccagatagt cgcggtagga
     1441 gttggcggcg aacagctcgt tggcgaagtc ggcgatcggg cttcccatcg tcacgagctg
     1501 gaacggcaac acgtcgactt gacccgtgcc ggccgccttc tcgcgcgatc gaatgaagtc
     1561 ggcgatgcac aggaacctac cgcggtgctg acggggaaac gtgaaccgga accgttcggg
     1621 cgcatccggt ctgggctcgg tcagcacgat cacgtcgtcg ccgtcggaca ccgccgggaa
     1681 gtacccgtac accaccgcgg cgtgagcgag gatgccgtcg gtggacagcc gatccaacca
     1741 gtaccgcagc cgcggccggc ctgccgactc gacaagctcc tcgtacgacg gtccttcgct
     1801 gccgcgggcg ccgcgcaatc cccactggcc caggaacagt gcacgttcgt cgagcaggcc
     1861 cgtgtattcg gccagcgcga cgcccttgac aatcctcgtg ccccagaacg gcggaacagg
     1921 tacttcgttg tcggcggcga catcggaccg ctccggcacc tcgaccggcg tctccttggc
     1981 tttacgttcg gccgcaatgc gtttcgaccg ctcgtggcgt gccttgcgct cggcttcctt
     2041 ctcccgcgcc gcgacggcct tcgggctgtc ggtatccggc gccacaccgc gtttggcgtt
     2101 catgatctgg tccatcaggt gcaggccctc gaacgcgtca cgtgcgtaat gcacgtcgcc
     2161 gctgtagatg tcggccaggt cgttctccac gtagctgcgg gtcaacgcgg cgccgccgag
     2221 cagcaccgga aacttgtccg ccaccccgcg gtggttcatc tcctcgaggt tttccttcat
     2281 caccacagtg gacttgacga gcagccccga catgccgacc acgtcggcgc tcttgtcctg
     2341 cgccacttcg agaatggtgt tgatcggctg cttgatgccg atgttgacca cttcgtagcc
     2401 gttgttgctc aagatgatgt cgacgaggtt cttgccgatg tcgtgcacgt cgcccttgac
     2461 ggtggccagc acgatgcggc ccttgccggc gtcgtcgtcg gagcgctcca tgtgcggctc
     2521 aaggtaggcc accgcggttt tcatcacctc ggcggactgc agcacgaacg gcagctgcat
     2581 ctggcccgaa ccgaacagct cgccaacggt tttcatgccc gacagcaggt cgtcgttgat
     2641 gatgtccaac ggcggtttct gcgtcatcgc ctcgtcgagg tcggcctcca ggccgttacg
     2701 ttcgccgtcg acgatgcgcc gggcgagccg ctccagcagc ggcagtgcgg ccaattctgc
     2761 ggcccgggac tccttcgacg acgacgcgga cacgccttcg aacatctgca tgagttcctg
     2821 cagcgggtcg tagccgtcgc ggcgacggtc gtagacgagg tcgagcgcta cgtcgcgccg
     2881 gtcctcggga atccggttca tcggcagaat cttcgacgcg tgcacgatcg ccgaatccag
     2941 tccagcctcc tgacactcgt gcaggaacac cgagttgagc acctgccgcg ccgccgggtt
     3001 cagaccgaac gagatgttcg acaagccgag cgtggtctgc acgtcaggat gacgcttctt
     3061 caattcgcgg atcgcctcga tggtctcgat gccgtcgcga cgggattcct cctggccggt
     3121 gcagatcgtg aacgtcaggc agtcgatgag aatggaggat tcgtcgactc cccaattcgt
     3181 cgtgatgtcg tcgatcagcc gctcggcgat ctgcaccttc ttgtccgcgg tgcgggcctg
     3241 gccctcctcg tcgatcgtca gcgccaccac cgcggcgccg tgttcggcga ctagctccat
     3301 ggtcttgtgg aaccgcgact cgggtccgtc gccgtcctcg taattcaccg agttgatcgc
     3361 gcaccggcca ccgagatgct ccaaaccggc ttgcagcacc ggcgtttcgg tggagtcgag
     3421 catgatcggc agcgtcgacg cggtggccag cctgctcgcc agtgcggtca tgtccgcgac
     3481 accgtcgcgg ccgacatagt cgatgcacag atccagcagg tgcgcgccgt cgcgggtctg
     3541 ctccttggcg atgtcgaggc accgctcgta gtcctcggcg atcatcgcat cgcgaaaagc
     3601 cctggagccg ttggcgttcg ttcgttcgcc gatcatcagc accgaggcgt cctgcgcgaa
     3661 cggcgtcgcg gcatacagcg acgacaccgc gggctcgtac gtcacgtgcc gcgccgcgcg
     3721 ctcgactcca cggaccgcgt cggcgacctg gcggatgtgt tcgggcgtcg tgccgcagca
     3781 tccgccgacc agcgacagcc cgaactcggt gatgaacccg gacagggcct cggccagctc
     3841 ttccggcgtg agcgggtatt ccgcaccgtt ggcacccagc accggcagcc ccgcgttcgg
     3901 catcaccgat accgggatgc gcgcgtggcg ggacaggtgg cggaggtgct cactcatctc
     3961 ggccgggccg gtggcgcagt tcaggccgat catgtcgacg ccgagcggct cgaccgcggt
     4021 cagcgccgcg ccgatctcgc tgccgagcag catcgtgccc gtcgtctcca ccgtcacatg
     4081 cgcgaacacc gggatgtgcc ggcccgcctg cgccatcgcg cgccgcgagc cgagcaccgc
     4141 ggccttgagc tgcaacaggt cctggcacgt ctcgaccagg attgcgtcgg cgccgccgtc
     4201 cagcatcccg agtgcggcct ccgtgtacgc gtcgcggatg acggcgtact cggtgtgacc
     4261 caacgtcggc agcttggtgc ccggccccat cgagccgagc acgtagcgct tggcctcagg
     4321 tgtgctgagc tcgtcggcga cgcggcgggc ggtcgcggtg cccttctccg acagctcacg
     4381 gatccggtcg gcgatgtcgt agtcacccag gttggacagg ttgcagccga acgtgttggt
     4441 ctcgaccgcg tcggcaccgg cctcgaaata cgcccggtga atgtgctcca gcacatcggg
     4501 ccgggtgtcg ttgaggattt cgttgcagcc ctcgaggccg ttgaagtcgt cgagcgtcag
     4561 atccgctgcc tgaagcatcg tgcccatagc gccgtcgccc accacgacct tctcagaaag
     4621 ggtcgccagc aggtcggtgt catagtcccg acgagatgca gcggtcaccc taacagggta
     4681 gtgggtctac cacatcgggg atttcgccaa gccgctcgcg gtgtcaatac acgctctacg
     4741 ctgaagtaat gaccccgtcc gacgctacaa gaggcttcga actacccgtg ctgcacgatg
     4801 ccgtggtggt cgcggccttc gaggggtgga acgacgcagg cgacgccgcg agcgacgccc
     4861 tggagcatct cgacgcgatc tgggaagcgg agccgatcat cgagatcgac gacgaggcct
     4921 actacgacta ccaggtgaat cgaccggtca tccggcaggt ggacggcgtc acgcgggaac
     4981 tggtgtggcc gtcgatgcgg atttcgcact gccgcccgcc cggcagcgag cgcgacatcg
     5041 tgctgatgca cggcgtcgag ccgaacatgc ggtggcgcac gttctgcgcc gaactgctgg
     5101 cgatcgccga caaactgaac gtggcgacgg tcgtcatcct cggcgcgctg ctggccgaca
     5161 cgccgcacac caggccggtg ccggtgtcgg gtgcggcgta ctcgccggag tccgcgcggt
     5221 tcttcgggct ggaggaaacg cgctacgagg gcccgaccgg catcgcgggc gtgttccagg
     5281 atgcatgcgt gaacgccggg attcccgcgg tgacgttctg ggccgcggtc ccgcactacg
     5341 tgtcgcaacc gccgaacccg aaggcgacgg tcgcgctact ccagcgggtc gaagacgtac
     5401 tcgacatcga agtgccgctg gccgacctgc ccgcgcaggc cgaggagtgg gaggccgcgg
     5461 tcaccgagat gacgtccgac gacgacgaga tcgccgagta cgtcgctcag ctcgaagagc
     5521 gcggcgacgc tgaggtcgac atgaccgagg cgctgtccaa ggtcgacggc gacgcgctgg
     5581 ccgcggagtt cgagcgctac ctgcgccgcc gcggcccggg cttcagaggc t
//
LOCUS       DASWBF010000224         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_82124, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000224 DASWBF010000000
VERSION     DASWBF010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82124"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VF874_07900"
     CDS             complement(<1..449)
                     /locus_tag="VF874_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9176007.1"
                     /translation="MKRVWIITLALVLAACGGGQKGNFSEPTDRLEVMSWWVSPSEHP
                     ALEVLLNAFKAANPNVDVIDASIEGGAGSNVQVALAARLRAGDPPDVWQTFVGSSLRA
                     WVNASRIADVSGVYERTNLAPTLPPTLRDAATYRNKQWGVPTGSHRG"
     gene            complement(446..1771)
                     /gene="fucP"
                     /locus_tag="VF874_07905"
     CDS             complement(446..1771)
                     /gene="fucP"
                     /locus_tag="VF874_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018121520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HEX9176008.1"
                     /translation="MTTTEHADQPSEAPASTSKPGLVFPKMWVVFAVLICCFTAWGVA
                     ADLTTPMVAGFKRIFDMSTFQASLVQLAYFGAYFLLAIPAALINERFGYKAGLLTGVL
                     LAAAGAIAFYPASKIMTYEAFLVALFAIAAGCSILETSANPYVLSLGPEETATRRLNF
                     AQAFNPVGTNIGVLLAATLILPKLDEPVNMADLTPAQARAIRAGELGAVMGPYLGLGF
                     VLIAIGLVIAINKSPPIVEEFESGDLEGKSPLRILLSNKHYVFGVVAQFFNVAAQVCT
                     WTYLIQYSQQALHGSLQLGGFLLQISLIVFLISRFVMTWVIGRVRATKAMMILGSLAV
                     VLCIFAMFSPNVAGVAAVVALSFCLSLMFPTIYGVALQGLGPATKFGAAGLVMAIVGG
                     AIMPLIQGRLLDATSPAISFIVPAVCFAVVTSFAVFDLKAVRHTAGERQ"
     gene            complement(1837..>2594)
                     /locus_tag="VF874_07910"
     CDS             complement(1837..>2594)
                     /locus_tag="VF874_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9176009.1"
                     /translation="DYLHDSGVDLAAGSTGASRTATAHAELDEKGAATYTFDIDWQIS
                     PNVSRTEPLVLHTGSIATALMPGASAVVELVDKHKSAATITFDPNIRSAFYESPEQAL
                     ASVENLVARADVVKASDEDMRWLDPESAPEELAARWLALGPAIVAVTFGADGSYAICT
                     DGAQRIPAYPAEVVDTVGAGDSFMTGLIDALWDAGLLGANRRAQLRDIRADDLRRVLQ
                     IAALSSALTVARPGAALPDRATRDRAAQRLLAE"
BASE COUNT          477 a          804 c          887 g          426 t
ORIGIN      
        1 tttccgcgat gcgaccccgt gggcaccccc cactgcttgt tgcggtaagt ggcagcgtca
       61 cgcaacgtcg gcggcagagt aggtgccaga ttcgtgcgct cgtaaacccc cgacacgtcg
      121 gcgattcgac tggcgttgac ccatgcacgg agggaactgc cgacgaacgt ctgccacacg
      181 tcaggaggat cacccgcccg caggcgggcc gcgagcgcga cttggacgtt gctgccggcg
      241 ccgccttcga tggacgcatc gatcacatcg acgttcgggt ttgccgcttt gaacgcgttc
      301 agcaagacct cgagggctgg atgttcggat ggcgacaccc accacgacat gacctcgagc
      361 cggtcggtcg gttcggaaaa gttgcccttc tgtccgcccc cgcaggccgc gagaaccagt
      421 gcgagcgtga tgatccagac gcgtttcatt gtcgttctcc cgcggtgtgg cgtacggcct
      481 tcaggtcgaa gacggcgaaa ctggtgacga ccgcgaagca gacggcgggc acgatgaagg
      541 agatcgccgg actggtcgcg tcgagaagtc ggccttgaat gagcggcatg atcgcgccgc
      601 cgacgatcgc catcaccaga ccggcggcgc cgaatttcgt tgcgggaccg agtccttgaa
      661 gggcgacacc gtagatcgtc gggaacatca gcgacaggca gaaggacaat gcgacgacgg
      721 cggcaacgcc ggccacgttc ggcgagaaca tggcgaagat gcagagcacg accgcgagcg
      781 agccgagaat catcatcgcc ttggtcgcgc gaacccgccc gatcacccac gtcatgacga
      841 accgcgagat gagaaagacg atcaagctta tctggagaag aaagccaccc aactgcagtg
      901 agccgtgcag agcctgctgc gagtactgga tcagatacgt ccacgtacac acttgcgctg
      961 cgacattgaa aaactgtgcg acgacgccga atacatagtg cttgttcgac agcagaatcc
     1021 gcagtggtga tttcccctcg aggtcgccgg actcgaactc ttcgacgatc ggcggggatt
     1081 tgttgatggc aatgaccaac ccgatggcga tcaacacgaa tccgaggccg agatagggac
     1141 ccatcacggc accgagctcg ccggcgcgga tcgcccgcgc ctgggccggc gtcagatcgg
     1201 ccatgttcac cggttcgtcc aacttcggca ggatcagggt cgcggccagc agcacaccga
     1261 tgttggtgcc gacggggttg aaggcctggg cgaagttgag tcgccgggtc gcggtctctt
     1321 cgggacccag cgaaagcacg taagggttcg cggacgtttc gagtatcgag cacccggcgg
     1381 cgatcgcgaa caaggcgacc aggaacgctt cgtaggtcat gatcttgctc gccgggtaga
     1441 acgcgatcgc ccccgccgcg gcgagcagaa cgcccgtgag gagtcctgcc ttgtagccga
     1501 accgctcgtt gatgagtgcc gccggaatgg ccagcaggaa gtatgcgccg aaatacgcca
     1561 gctgaacgag agaggcctgg aaggtgctca tgtcgaagat gcgcttgaag ccggccacca
     1621 tcggggtggt gaggtcggcg gcgacgcccc atgcggtgaa acagcagatc agcaccgcga
     1681 agaccaccca catcttgggg aagaccagac ccggtttgga cgtcgacgcg ggcgcttcgg
     1741 agggttgatc ggcgtgctcg gtggttgtca tactcagccc tctcgacctg tgatctccat
     1801 cacgctactc gcgtggttac ccccgggaag tgcgtttcac tcggcgagga ggcgctgcgc
     1861 cgcccggtcg cgagtggcgc gatcaggcag ggcagccccc ggccgcgcga cggtgagcgc
     1921 cgacgacagc gcggcgatct ggagcacccg gcgcaggtcg tcggcgcgga tgtcacgcag
     1981 ttgcgcgcgg cggttcgcgc cgagcaggcc ggcgtcccac agcgcgtcga tcagtccggt
     2041 catgaacgag tcgccggcac ccaccgtgtc gacgacctcg gccgggtacg ccggtatgcg
     2101 ttgcgcgccg tcggtgcaga tggcgtagga gccgtccgcc ccgaacgtga ccgcgacgat
     2161 cgccggcccg agcgccagcc agcgggctgc gagttcctcg ggcgccgact ccggatcgag
     2221 ccagcgcatg tcctcgtcgc tggccttcac cacgtcggcg cgggctacta ggttttcgac
     2281 gctggccagt gcctgctccg ggctctcgta gaacgcggac cggatgttgg ggtcgaaggt
     2341 gatggtggcc gccgacttgt gcttgtcgac cagctcgacg accgcgctgg cgcctggcat
     2401 gagggcggtg gctatcgagc cggtgtgcag caccaggggt tcggtgcggc tgacgttcgg
     2461 cgagatctgc cagtcgatgt cgaaagtgta agtggccgcg cctttttcat ccagctcggc
     2521 gtgtgcggtg gcggtgcgag atgccccggt gctgccggcg gcgaggtcga caccgctgtc
     2581 gtgcaggtag tcgg
//
LOCUS       DASWBF010000225         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1004920,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000225 DASWBF010000000
VERSION     DASWBF010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004920"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VF874_07915"
     CDS             complement(<1..498)
                     /locus_tag="VF874_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176010.1"
                     /translation="MLAFTPWGLLPAAVPLGWVTVRGLGALVLPRAGMQSVLAGATLT
                     VALIAVWVRLLGAVGLLTTWVLVGSLTVVAAAVIVTVRLRGVPWRLPWRRAVSAETIP
                     VLMISLVALVIAVVAGYYLPVWQWDALGYHLPYVNFALQRGTLADLPSDMPYLSTYPH
                     IVEYLF"
     gene            complement(525..953)
                     /locus_tag="VF874_07920"
     CDS             complement(525..953)
                     /locus_tag="VF874_07920"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX9176011.1"
                     /translation="MIPDINYLAVLVAVIVSMVIGLVFYHRAVFGRAWMNLVGHTDET
                     LGSGSPLTYPVVVVASFLTAWVLAGASFLAYKFYEGSFLVSALVTGWILWLSFTVARM
                     LVHDLFDTRSLKLTALNALNEFITVTAMALIIGLWPPPGV"
     gene            complement(1006..1665)
                     /locus_tag="VF874_07925"
     CDS             complement(1006..1665)
                     /locus_tag="VF874_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HEX9176012.1"
                     /translation="MPSYLLRVQLVDRPGSLGSLAVALGSAGADILSLDVVERMSGFA
                     IDDLVVDLPPGAMPDTLITAAEALNGVRVDSIRPHTGLLEAHRELELIDHIAAGPDRL
                     GKLQVLADEAPRVLRVGWCTVVRLHDHGFERVVGSPSSPETVAEEAPWLPLEHPAALD
                     GTGDWVPQVWRDMDTTLAAAPLGDPSCAVMLGRPGGPGFRPSEVARLGYLAGIVATIL
                     R"
     gene            1729..2028
                     /gene="gatC"
                     /locus_tag="VF874_07930"
     CDS             1729..2028
                     /gene="gatC"
                     /locus_tag="VF874_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX9176013.1"
                     /translation="MAQISRDEVARLAKLARLALTDAELDSFAGQLDAIIGYVSQVQA
                     VDVTDVKPTGNPLKDVNVTRPDVVQPCLTQKQALAEAPEAVNGRFAVPRILGEPE"
     gene            2025..>3335
                     /gene="gatA"
                     /locus_tag="VF874_07935"
     CDS             2025..>3335
                     /gene="gatA"
                     /locus_tag="VF874_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX9176014.1"
                     /translation="MTELVRLDAATLAEKIADREVSSVEVTQACLDQIEATDARYHAF
                     LHVAADQALATAARVDAADEPVSPLAGVPLALKDVFTTTDMPTTCGSKILDGWRSPYD
                     ATVTARLRAAGIPILGKTNMDEFAMGSSTENSAYGPTYNPWDVERVPGGSGGGSAAAL
                     ASFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTV
                     LDTALLHAVIAGHDPKDSTSVEAAVPDVVAAARAGAVGDLTGVRIGVVSQLRGDGYQP
                     GVLESFNTAVEQLTALGAEVSEVSCPHFEHALAAYYLILPSEVSSNLARFDGIRYGLR
                     VGDDGAHTTEEVMALTRAAGFGPEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARD
                     LDEAYQKVDVLVSPATPTTAFRLGEKVDDPLAMYLFDLCTLPLNL"
BASE COUNT          601 a         1165 c         1111 g          458 t
ORIGIN      
        1 gaacagatat tccacgatgt gtggataggt ggacagatac ggcatgtccg acggcagatc
       61 ggccagcgtg ccgcgctgca gcgcgaagtt cacgtacggc aggtggtagc cgagcgcgtc
      121 ccactgccac accggcaggt agtacccggc gacgaccgcg atgaccagcg cgacgagtga
      181 gatcataagc accggaatgg tttccgcgct gaccgcacga cgccatggca gtcgccacgg
      241 cacaccgcgt agccgtacag taacgatgac ggcggcggcg acaaccgtca gcgagccgac
      301 cagcacccag gtcgtcagca gacccaccgc gccgagcagc cgcacccaca ccgcgatcag
      361 cgccaccgtc agcgtcgcgc cggccaacac cgactgcatt ccggcccgcg gtagtaccag
      421 cgcgccaagc cctcgaaccg tcacccaacc cagcggtacc gcagccggaa gcagccccca
      481 cggggtgaat gccagcatcg aaatagcttg ttgcgtaagg gagctcagac gcctggcggc
      541 ggccaaaggc cgatgatcaa cgccatcgcg gtgaccgtga tgaactcgtt gagcgcgttg
      601 agtgcggtga gcttcaacga cctggtgtcg aagaggtcat gtaccagcat ccgggcgacc
      661 gtgaagctca accacagaat ccacccggtg accaacgccg agaccagaaa gcttccctca
      721 tagaacttgt aggccaggaa actggccccg gctaacaccc atgccgtgag aaaggacgca
      781 accacgacaa cgggataggt cagcggtgaa ccgctcccca gcgtttcgtc ggtgtgtccg
      841 accagattca tccacgcgcg accgaaaacg gcccgatggt agaagaccag gccgatcacc
      901 atgctgacga tcacagcgac caggaccgcg aggtagttga tatcggggat catgtcgacc
      961 tctctgccag cgcctgaacc gattagagca gagatggtga ggcgttcagc gcagaatcgt
     1021 cgcgacgatg ccggccagat agcccagccg ggccacctcc gacgggcgga aacccgggcc
     1081 gccgggacgg cccagcatca ccgcgcagct cggatcgccc agcggcgccg ccgcgagcgt
     1141 ggtgtccatg tcgcgccaca cctgcggcac ccagtcgccg gtgccgtcca gcgcggcggg
     1201 gtgttcgagt ggcagccacg gcgcctcttc ggcgaccgtt tccggcgagc tcggactgcc
     1261 cacgacccgc tcgaagccgt ggtcgtgcag tcggacgacc gtgcaccacc cgacccgcag
     1321 cacccgcggt gcctcgtcgg cgagcacctg cagtttgccc agtcggtccg gcccggcagc
     1381 gatgtggtcg atcaattcga gctcgcggtg cgcctccagc aggccggtgt gcggccgaat
     1441 gctgtccacc cggacgccgt ttagcgcctc ggccgcagtg atcagcgtgt cgggcatcgc
     1501 gccgggaggc agatccacga cgaggtcgtc gatcgcgaag cccgacatcc gctcgacgac
     1561 gtcgagcgac aggatgtccg cccccgccga gccgagcgcc acggccagcg agccgaggct
     1621 gcccggccgg tcgaccagct ggacccgcag cagatacgaa ggcacgggca acactgtcgc
     1681 acagcacgct cggccgggca tttcaagcga ccactaggct gtccgaccgt ggctcagatc
     1741 tcccgcgacg aggtggcccg gctggccaag ctggcgcgcc tggccctcac ggacgccgag
     1801 ctggacagct tcgccgggca actcgacgcg atcatcggct acgtcagcca ggtgcaggcc
     1861 gtcgacgtga ccgacgtcaa gcccaccggc aacccgctca aggacgtcaa cgtgactcgt
     1921 cccgacgtcg ttcagccgtg tctgacccag aagcaggcgc tggcggaggc gcccgaagcg
     1981 gtgaacggcc ggttcgcggt gccgcggatc ctgggggagc cggagtgacg gagctggtcc
     2041 gcctcgacgc cgcaacgctg gccgaaaaga tcgcggaccg ggaggtgtct tcggtcgagg
     2101 tgacgcaggc ctgccttgac cagatcgagg ccaccgatgc gcggtatcac gcgtttctgc
     2161 acgtcgcggc cgatcaggcc ctggcgactg cggcccgggt ggatgccgcg gacgagccgg
     2221 tctcgccgct ggccggcgtg ccgttggccc tcaaggacgt gttcaccacc actgacatgc
     2281 cgacgacctg cggctcgaag atcctggacg gctggcgctc gccctacgac gcgacggtga
     2341 ccgcgcggct gcgggccgcg ggcatcccga tcctcggcaa gaccaacatg gacgagttcg
     2401 cgatgggcag ctcgaccgag aactccgcgt acgggccgac ctacaacccg tgggacgtcg
     2461 agcgggttcc gggcgggtcc ggcggcggca gcgccgcggc gctggcctcg ttccaggcgc
     2521 cgctcgcgat cggctcggac accggcggct ccatccgcca accggccgct ctgaccgcga
     2581 ccgtgggcgt caagcccacc tacggaacgg tgtcgcgcta cgggctggtg gcgtgtgcgt
     2641 cgtccctgga ccagggcgga ccctgcgcgc gcaccgttct cgacaccgcg ctgctgcacg
     2701 ccgtaatcgc cggccacgac ccgaaggact ccacgtccgt cgaggcggcc gtgcccgacg
     2761 tcgtcgcggc cgcacgagcc ggagccgtcg gcgacttgac cggtgtgcgc atcggcgtcg
     2821 tgagccaact gcgcggcgac ggctaccagc cgggagtgtt ggagtcgttc aacaccgcgg
     2881 tcgagcagct gaccgcgctg ggcgccgagg tcagcgaggt gtcctgcccg catttcgagc
     2941 acgcgctggc cgcctactac ctgatcctgc cgtcggaggt gtcgtcgaac ctggcgcggt
     3001 tcgacggaat ccggtacggc cttcgcgtcg gcgacgacgg cgcgcacacc accgaagagg
     3061 tgatggcgct gacccgcgcc gcgggattcg gcccagaagt caagcggcgc atcatgattg
     3121 gcacatacgc gctgtcggca ggctactacg acgcgtatta caaccaggcg cagaaagtgc
     3181 gcacgctgat cgcgcgcgat ctcgacgagg cgtaccagaa ggtcgacgtg ctggtctcgc
     3241 cggcgacacc caccaccgcg ttccggctgg gagagaaggt cgacgacccg ctggcgatgt
     3301 acctgttcga cctgtgcacg ctgccgctca acctc
//
LOCUS       DASWBF010000226        24224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_542570,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000226 DASWBF010000000
VERSION     DASWBF010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24224
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542570"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VF874_07940"
     CDS             <1..325
                     /locus_tag="VF874_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9176015.1"
                     /translation="AAGPCRTRNLPLVGAQGGAQSVGLPTSLVARYGAEAPSVIGSAT
                     CERPAESVTDEIDVTRAEFEYAVSHEGALNVDDILDRRTRIGLVKQDRDRVVAVAEEF
                     VAQVR"
     gene            complement(322..465)
                     /locus_tag="VF874_07945"
     CDS             complement(322..465)
                     /locus_tag="VF874_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX9176016.1"
                     /translation="MIIQRASHWAIGTAMAVTLLYFASIATAETVADAFEPDLESWDV
                     LSS"
     gene            complement(603..2024)
                     /locus_tag="VF874_07950"
     CDS             complement(603..2024)
                     /locus_tag="VF874_07950"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9176017.1"
                     /translation="MTIMAPEAVGESLDPRDPLLRLSTFFDDQSLELLHERDRSGVLA
                     AAGTVNGVRTVAFCTDGTVMGGAMGIEGCRHIVDAYDLAIEEQSPIVGIWHSGGARLA
                     EGVKALHAVGLVFEAMIRASGYVPQISVVVGFAAGGAAYGPALTDVIVMAPESRVFVT
                     GPDVVRSVTGEDVDMASLGGPETHHKKSGVCHIVADDEVDAYERGRRLVGLFCQQGHF
                     DRDKAEAGDTDLHALLPESPRRAYDVHPLVNALLDDDAPLEEFQAKWAPSMVIGLGRL
                     SGRTVGVLANNPLRLGGCLNSESAEKAARFVRLCDAFGIPLVVVVDVPGYLPGVDQEW
                     GGVVRRGAKLLHAFGECTVPRVTLVTRKIYGGAYIAMNSRSLGATKVFAWPDSEVAVM
                     GAKAAVGILHKKKLAAAPEHEREALHEQLAEEHESIAGGVDSAIDIGVVDAKIDPAHT
                     RSALTQALAEAPPRRGRHKNIPL"
     gene            complement(2097..3350)
                     /gene="kasB"
                     /locus_tag="VF874_07955"
     CDS             complement(2097..3350)
                     /gene="kasB"
                     /locus_tag="VF874_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase KasB"
                     /protein_id="HEX9176018.1"
                     /translation="MTRLSTGNGFPNVVVTGIAMTTALATDADTTWKKLLDGQSGIRK
                     LEDPFVEQYSLPVRIGGHLLEDFDPELTKVELHRLSYLQKMSTVLSRRVWQNAGSPEV
                     DPRRLMVSIGTGMGSTEELVFSYDDMRARGMRAVSPLAVQKYMPNAAAAAVGLERKAR
                     AGVSTPVSACASGSEGIAQAWRNLVLGEADVAICGGVESKIEAVPIAAFAQMRIVLST
                     TNDDPAGACRPFDKDRNGFVFGEAGALIVIETEEHAKARGANILGRLMGASITSDAFH
                     MVAPDPNGEQAGWAMTRAIQLAGLTPTDIDHVNAHATGTSVGDVAEAKAIRNALGSHD
                     AAVYAPKSALGHSVGAVGAVEAILTVLALRDGVIPPTLNLKNQDPEIDLDVVHGEPRV
                     GNYRYAINNSFGFGGHNVALAFGKY"
     gene            complement(3391..4641)
                     /gene="kasA"
                     /locus_tag="VF874_07960"
     CDS             complement(3391..4641)
                     /gene="kasA"
                     /locus_tag="VF874_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase KasA"
                     /protein_id="HEX9176019.1"
                     /translation="MTKPSTANGGFPSVVVTAVTATTSISPDIESTWKGLLAGESGIR
                     VLEDEFVTKWDLPVRIGGHLKEPVDDLMNRLDLRRMSYVQRMAKLISGRLWESAGTPE
                     VDGDRFAVVVGTGLGGGEKIVETYDLMNEGGPRKVSPLAVQMIMPNGAAAVVGLQLGA
                     RAGVITPVSACSSGSEAIAHAWRQIVMGDADIAVCGGVEGGIEALPIAAFSMMRAMST
                     KNDDPEGASRPFDKNRDGFVFGEAGALMVIETEEHAKARGAKPLARLLGAGITSDAFH
                     MVAPAPDGERAGKAMQRALELAGLSAKDIDHINAHATATPIGDTAEANAIRVAGCEHA
                     PVYAPKSALGHSIGAVGALESVLTVLTLRDGVIPPTLNYETPDPEIDLDVVAGEPRYG
                     DYKYAINNSFGFGGHNVALAFGKY"
     gene            complement(4638..4940)
                     /gene="acpM"
                     /locus_tag="VF874_07965"
     CDS             complement(4638..4940)
                     /gene="acpM"
                     /locus_tag="VF874_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="meromycolate extension acyl carrier protein
                     AcpM"
                     /protein_id="HEX9176020.1"
                     /translation="MPATQEEIIAGLAEIIEEVTGIEPSEVTPEKSFVDDLDIDSLSM
                     VEIAVQTEDKYGVKIPDEDLAGLRTVGDVVGYIQKLEEENPEAAEAIRAKIEADRT"
     gene            complement(5014..5934)
                     /locus_tag="VF874_07970"
     CDS             complement(5014..5934)
                     /locus_tag="VF874_07970"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9176021.1"
                     /translation="MIALLCPGQGSQTPGMLSPWLELPGASETLTSWCEVSGLDLVTL
                     GTTATAEEITDTAVTQPLVVAATLLAHQELVRRNLLARREISREVLVAGHSVGEIAAY
                     AIGGIISADDAVALAATRGVEMAKACALEPTGMSAVLGGEETEVLARLDELELVPANR
                     NAAGQVVAAGRLDALAKLAENPPEKARVRLLATAGAFHTHFMSSALDAYTAAAANVQT
                     AEPTAVVLSNADGQPVASAEDAMTKLVAQMTRPVRWDLCTETLRQRNVTAIVEFPPAG
                     TLTGIAKRELRGIGTHAVKSPADLDGLAAF"
     gene            complement(6076..7353)
                     /locus_tag="VF874_07975"
     CDS             complement(6076..7353)
                     /locus_tag="VF874_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEX9176022.1"
                     /translation="MPDKDRPAPPLSTLELLETVPESLLRKLKQYSGRFATEAVAAMQ
                     ERLPFFADLEASQRASVHLVVQAAVVNFVEWMGNPHGNVGYTVQAFELVPQDLTRRIA
                     LRQTVDMVRVTMEFFEEVVPMLARSEEQATALTTGILRYSRDLAFTAASAYADAAEAR
                     GSWDSRMEANLVDAVVRGDAGPELQSRAAALNWDTTAPATVVVGLPRAERVDFASDDV
                     REIAQRHGRAALSDVHGTWLIAIVSGPLAPTDKFLRELIDVFADGPVVIGPTAPTFTA
                     AHTSATEAIAGMNAVAGWSSAPRPVLARELLPERALLGDASAIAVLDAEVMRPLADAG
                     PALTETLDAYLDSGGAIEACARKLFVHPNTVRYRLKRITDFTGRDPTTPRDAYVLRVA
                     ATVGRLASQATNPTSAYPSVAQITPPRGVHRAG"
     gene            complement(7461..10250)
                     /gene="aceE"
                     /locus_tag="VF874_07980"
     CDS             complement(7461..10250)
                     /gene="aceE"
                     /locus_tag="VF874_07980"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX9176023.1"
                     /translation="MTTEFARRDLAQNSTGSNESDRVRVIREGVASYLPDIDPEETGE
                     WLESFDDVLDRSGPARARYLMLRLLERAGEQRVAIPALTSTDYVNTIPTELEPWFPGD
                     EEVERRYRRWIRWNAAIMVHRAQRPGVGVGGHISTYASSAALYEVGFNHFFRGKSQPG
                     GGDQVYIQGHASPGIYARAFLEGRLTADQLDGFRQEHSHPGGGLPSYPHPRLMPEFWE
                     FPTVSMGLGPLNAIFQARFNHYLHDRGIKDTSDQHVWAFLGDGEMDEPESRGAAHVAA
                     LEALDNLTFVINCNLQRLDGPVRGNGKIIQELESFFRGAGWNVIKVVWGREWDALLHA
                     DRDGALVNLMNTTPDGDYQTYEANDGAYVREHFFGRDPRTKALVQNMSDDEIWSLKRG
                     GHDYRKVYAAYRAATEHKGQPTVILAHTIKGYSLGKHFQGRNATHQMKKLALEDLKGF
                     RDAQRIPVSDKQLDENPYLPPYYHPGPDAAEIRYMLDRRRALGGFVPERRTKSKALTL
                     PPRDIYKSVKKGSGHQEVATTMATVRVFKELLRDKEIGPRIVPIIPDEARTFGMDSWF
                     PSLKIYNRNGQLYTSVDAEMMLAYKESEIGQILHEGINEAGSTATFTAAGTSYATHNE
                     PMIPIYIFYSMFGFQRTGDGFWAAADQMARGFVLGATAGRTTLTGEGLQHADGHSVLL
                     ASTNPAVVAYDPAFAYEIAYIVESGLARMFGENPENVYFYMTIYNEPYPQPAEPEDLD
                     VEGLLRGIYRYRPAPEPRQYSAQILASGVSMPDALRAADLLAADWDVAADVWSMTSWG
                     ELNREGVEIEKERLRHPDHSPRVPYVTQALADARGPVVAVSDWMRAVSEQIRPWVPGT
                     YITLGTDGFGFSDTRPAARRFFNTDAESIAVGVLEGLARDGEIDKSVAVEAARRYEID
                     DVLAAPEQTSDPGVA"
     gene            10340..10915
                     /locus_tag="VF874_07985"
     CDS             10340..10915
                     /locus_tag="VF874_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176024.1"
                     /translation="MLGVGGVRRWRIAALVAGLIAAAALVVVGCTSMTSGTPAVDTND
                     ARDYRTWVVDSLSQSAAASSERESTRQVSLTAEAVANACEVLSNTSVDAVTAINAYVS
                     AVAAHNEADVAAKAQAAVDTLNRGADAVAATLNSALLPHMRDAFTAWVDATRAVAKAI
                     GERYGQQEFNAEVDKFNQANDAALNTCGGSP"
     gene            11006..11428
                     /locus_tag="VF874_07990"
     CDS             11006..11428
                     /locus_tag="VF874_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEX9176025.1"
                     /translation="MVAAGDAPNYARRLGIEKDQVVQELGWDEDTDDDIRADIEDACG
                     SELLDDDSDEVIDVVLLWWRDGDGDLVDRLMDAITPLSDDGVIWVLTPKTGKPGHVLP
                     AEISESAPTAGLMQTSSANLGDWSASRLVQPKSRAGRR"
     gene            11428..11889
                     /locus_tag="VF874_07995"
     CDS             11428..11889
                     /locus_tag="VF874_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9176026.1"
                     /translation="MLDVGTSAPDFTLRDQNQQPVSLSDFRDNKSVLLVFFPLAFTGV
                     CQGELDEIRDHLPEYENDETQTLAISVGPPPTHKVWATESGFTFPVLSDFWPHGAVAQ
                     AYGVFNDDAGFANRGTFVVDKSGIIRFAEMKQPGEARDQSLWKTALAALQS"
     gene            12004..12078
                     /locus_tag="VF874_08000"
     tRNA            12004..12078
                     /locus_tag="VF874_08000"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12036..12038,aa:Val,seq:tac)
     gene            complement(12082..15213)
                     /locus_tag="VF874_08005"
                     /pseudo
     CDS             complement(12082..15213)
                     /locus_tag="VF874_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305051.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(15332..15667)
                     /locus_tag="VF874_08010"
     CDS             complement(15332..15667)
                     /locus_tag="VF874_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176027.1"
                     /translation="MAENSTEKKPVAERAADLTGRAADLSDDVLKSVESGQRAAIDAV
                     RKFVGTVDESIPGHGDDGPSRRKTIVDAALDMADKLVKTQYEFIRSVVGSASEALNKE
                     GDAKPPAGG"
     gene            complement(15739..16113)
                     /locus_tag="VF874_08015"
     CDS             complement(15739..16113)
                     /locus_tag="VF874_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176028.1"
                     /translation="MAESTKKKTAAKRASDLSDEVLKRIEARQLAAIEAIRKLVDRLD
                     DATPDLVDDPSARKKVVGAIGDYAEQLTTTTNEYLRSAVRGASGMVSKPGEAKDLYGF
                     LRSFVRGGSETASKEDDAKRAD"
     gene            complement(16254..16589)
                     /locus_tag="VF874_08020"
     CDS             complement(16254..16589)
                     /locus_tag="VF874_08020"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9176029.1"
                     /translation="MSRVATFAVSLTALALTMCPVAQAEPTADDWYSKALSGNLVNYQ
                     GRASFEEMISLGQSVCSALDRSPGHSTFVSAVNNIVAKGVFNPTEARGIASSAVAAYC
                     PNHNDSNFG"
     gene            complement(16954..17736)
                     /locus_tag="VF874_08025"
     CDS             complement(16954..17736)
                     /locus_tag="VF874_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176030.1"
                     /translation="MGNSTTTLGLPSGFPCAGAQSFADIGPGAPVIVADEARKLLAKG
                     TFDSSYLVRDSCNLRFSISDVPGGAKFYRVQVAGQRESTYTEAEARAGAEISFASSEP
                     SSSATPTPTTTRTPTTTSSPRPTPTTVDSETAALNRLRAIADGDRSSVSLYLAERWVA
                     QISSKYYGLVAEGTTWDYAKILQEHLQLRQIYPGVRLLYSPDWSTFDTKYQWWVTVVG
                     ISYTDSRDAKAWCAARGFDRNHCFAKLISTTHPVEGSTDYNP"
     gene            complement(17932..18787)
                     /locus_tag="VF874_08030"
                     /pseudo
     CDS             complement(17932..18787)
                     /locus_tag="VF874_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293166.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
     gene            complement(18792..19463)
                     /locus_tag="VF874_08035"
     CDS             complement(18792..19463)
                     /locus_tag="VF874_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176031.1"
                     /translation="MNTKSTRRRKVGVMIALVGFILIIFGKLLAAAVLLAASAHAAPN
                     SDGRLYGDPTEAAEYWHPQHTGDCGLMAVADIVGQLTGKSPTEQQIIALAEQTPSVVR
                     PGQPVWTPPTDPNDITTASGSKAPDEVVLLAHYGIDAVVTDVEIAATSDGIPTGLEAL
                     ERYLASGTRCLSTSIRAHCMARASPPTPTTSWWSPASTPPPASCTSTTVWTRTAIPRS
                     PSTPS"
     gene            complement(19541..19942)
                     /locus_tag="VF874_08040"
     CDS             complement(19541..19942)
                     /locus_tag="VF874_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9176032.1"
                     /translation="MPDFPGLAHVALTVKDIEVSGPWYRRLLGADPVLDEHTDAGFRH
                     LVWLLDGGTLLGIHQHDRGVPDEHFSEFRVGLDHVGFACANRGELEKWMNRLNELGIS
                     HGGIVDAGYGSGLSFRDPDGIALEFFAPPSE"
     gene            complement(19952..20764)
                     /locus_tag="VF874_08045"
     CDS             complement(19952..20764)
                     /locus_tag="VF874_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX9176033.1"
                     /translation="MRRPNRVADLLNPAGLLLPAANVIMQLSAPAVGYGVLESPVDSG
                     NVYKHPFKRARTTGTYLAAATVGTDKDRESIRRAVDTVHAQVRSTARSPLSYNAFDPR
                     LQLWVAACLYRYFVDQHEFLYGPLDDDCADAVYRDAKRLGTTLQVPESMWPSDRIAFD
                     EYWKRSLDELRIDPPVRQHLYGVASLAFLPWPLRVIGGPLNLFATTGFLPAEFRTHMQ
                     LPWTSGQQRRFELLLTGLRLADRVIPRQLWLLGYQVYLRDMRSRTRRGRRIV"
     gene            20803..21762
                     /locus_tag="VF874_08050"
     CDS             20803..21762
                     /locus_tag="VF874_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019249810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX9176034.1"
                     /translation="MFGRRAVGLAAGWLADLAFADPPQHHPVAGFGAAAGALERRTYR
                     DVRWAGVVHAGLLLSAVGLLGVAVERVAGRIGIAAATWTALGGTSLLRTVAAMTDTLS
                     AGDVGAARRLLPSLCGRDPAALDADGLTRAALESLAENTSDAQVAPLLWTAVGGAPGA
                     LVYRAVNTLDAMVGYRSPRYARFGWAAARLDDFANFIPARFTAALVVMCAPLVGGSPT
                     GAVRAWRRDAKRHPSPNAGVVEAAFAGALGIRVGGPTRYPDGLEIRPTLGDGPAPRID
                     DLRRAVGLSRAVQAAAAVVAVACVSFASHTSTARPQWISLKKR"
     gene            21811..>24224
                     /locus_tag="VF874_08055"
     CDS             21811..>24224
                     /locus_tag="VF874_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9176035.1"
                     /translation="MSAPPSGVVTFLFTDVEGSTRRWEADADGMRAALAAHDEVLRGA
                     IEAHGGFMFKHTGDGVCAAFASPKSAVDAAVAAQGQLALPVRMGLATGEAELQEGDYF
                     GAVLNRAARVMAAGHGGQILLAESTAALLSGIDLIYLGPRRLRDLPGAIGVYQVRAPG
                     LQKDFPPLRALDASPGNLRPAVTSFVGREAEVAEVEAAVRAHRLVTLTGVGGVGKTRL
                     ATEVAAQVADEFLDGVWFFELAAVADPSAVPDAVAAVLGITQQPGKSVSESVAAALEG
                     RVRLLVFDNCEHVLDAAADLAEAIFTHSAIVRILATSREGLGVADEQVWPVPSLDVGA
                     GTDCAAVELLVQRARAVAPRFSMAERDEAAAVVEICRRLDGIPLAIELAASRMASMTA
                     SEVRDRLDHRFRLLVGSRRGLARHHTLRHAVGWSYDLLHDAEKALLDRCSVFAGGFNL
                     ESACAVAGSDDEFATMDLLDALVRKSLLTADRSAGRTRFSMLETIRQFAEEQLVVRGE
                     ATEARYAHARHFALREADIMALWDSPRQREAYDWFAAELANLRTAFRWAADQGDLDVA
                     AAIATYAGFLGYLVENYEPITWAEELIEPARAVDHPRLVFLYVMASLCWIVGRIEEAV
                     QHSDDGQVAMRSGRGEVPFGFETLLGSAYLAIGQADRCVEWCRAQTASSRDAHALGRA
                     SLAIALTLAGRGDEAMAAADGLVETAEATHNPYALSLALMAYAFACRDAYPDRALEAM
                     HRGLVIAQDSGNRFNETHLAATLAELEVERDDPLAALDHITLAIRNLHDSDNVVVMRS
                     PLTNLA"
BASE COUNT         3862 a         7948 c         8208 g         4206 t
ORIGIN      
        1 cgccgccggt ccgtgccgca cccgcaatct gccactggtc ggcgcgcagg gcggcgcgca
       61 atcggtcggt ctgccaacgt cgctagtcgc ccgctacggc gcggaggcgc cgagcgtgat
      121 cgggtcggcc acctgtgagc gccccgccga gtccgtcacc gacgaaatcg acgtcaccag
      181 agccgaattc gagtatgcgg tcagccacga gggggcactc aacgtcgacg acatcctcga
      241 tcggcgcacg agaatcgggc ttgtgaagca agaccgcgac cgcgtggtgg ccgtcgccga
      301 ggagttcgtc gcgcaggtgc gctagctgct caggacgtcc cagctttcga ggtcgggctc
      361 gaaggcgtcg gcgacggttt cggcggtcgc gatcgacgcg aagtacagga gggtgacggc
      421 catggcggtg ccgatcgccc agtgagaggc acgttgaata atcatgcgct tagcgtccaa
      481 cggacgcctt ggcgaattct tggagccgcc gagacagaaa ccagggctgc gattctcgaa
      541 gaatcacgac ccttgcttcg gtttcggcgc ggagaacggc cgcagagagg ggcgggagac
      601 ccctacagcg gaatgttctt gtgcctgccg cgacgtggcg gcgcctcggc cagggcctgc
      661 gtcagcgcgc tgcgggtgtg cgccgggtcg atcttcgcgt ccacgacgcc gatatcgatg
      721 gcgctgtcca cgccgccagc gatgctctcg tgctcctcgg ccagctgctc gtgcagcgct
      781 tcgcgctcgt gctcgggggc cgcggcgagc ttcttcttgt gcaggatgcc gacggccgcc
      841 ttcgcgccca tcaccgcgac ctcggaatcc ggccacgcga acaccttcgt cgcacccagc
      901 gaccgcgagt tcatcgcaat gtaggccccg ccgtagatct ttcgcgtcac cagcgtgacc
      961 cgggggacgg tgcactcgcc gaatgcgtgc agcagcttgg cgccgcggcg caccacgccg
     1021 ccccactcct ggtcgacccc gggcaggtac cccggcacgt cgacgacgac caccagcgga
     1081 atgccgaacg cgtcgcacag ccgcacgaaa cgtgctgcct tctcggcact ttcggagttc
     1141 aggcaaccgc ccagccgcag cgggttgttg gccagcacgc ccaccgtgcg gcccgacagc
     1201 cggcccagcc cgatcaccat cgacggcgcc cacttcgcct ggaactcctc gagcggcgcg
     1261 tcgtcgtcga gcagggcgtt gacgagcggg tgcacgtcgt aggcacgacg cggggattcg
     1321 ggcagcaacg catgcagatc ggtgtcgccg gcttcggcct tgtcgcggtc gaaatggccc
     1381 tgctggcaga acaatccgac caggcgacgg ccgcgctcgt acgcgtcgac ctcgtcgtcg
     1441 gcgacgatgt ggcacacgcc ggacttcttg tggtgcgtct ccggaccacc cagcgacgcc
     1501 atgtcgacgt cctcgccggt gacgctgcgc acgacgtcgg gtccggtgac gaacacccgg
     1561 ctctccggtg ccatcacgat gacgtcagtc agggccggcc cgtacgcggc gccgccggcc
     1621 gcgaagccga cgacgacgga gatctgcggg acgtagccgg acgcccggat catggcctcg
     1681 aacaccaaac ccactgcgtg caaagccttt acgccttcag ccagtcgggc accgcccgag
     1741 tgccagatgc cgacgatcgg gctctgctcc tcgatcgcga ggtcgtaggc gtcgacgatg
     1801 tgccggcagc cctcgatgcc catcgcgccg cccatgacag tcccgtcggt gcagaacgcg
     1861 acggtgcgaa caccgttgac ggtgcctgcc gcggcgagca cacccgatcg atcccgctcg
     1921 tgcagcagct cgaggctctg gtcatcgaag aacgtggaca gccgcagcag cgggtcgcgc
     1981 gggtcgagcg attccccaac cgcctcgggg gccatgatcg tcatcggtgt ctcctgtggt
     2041 tttggtctaa gagttcagca cggaccttgg agctttctaa aagcctttct ccatggttag
     2101 tacttcccga aggcgagtgc gacgttgtgc ccgccgaatc cgaacgagtt gttgatcgcg
     2161 taacggtagt tgccgacccg aggctcgccg tggacgacat ccaggtcgat ctcgggatcc
     2221 tggttcttca ggttcagggt cggcgggatc accccgtcgc gcagcgccag cacggtcagg
     2281 atcgcctcta ccgcgccgac cgcgccgact gagtggccga gcgccgactt cggcgcgtac
     2341 acggcggcgt cgtggctgcc gagggcattg cggatggcct tggcctcggc cacgtcgccg
     2401 accgaggtgc cggtcgcatg cgcgttgacg tggtcgatgt cggtgggcgt cagacccgcc
     2461 agttggatcg cgcgagtcat cgcccacccg gcctgctcgc cgttggggtc gggggccacc
     2521 atgtggaagg cgtccgacgt gatcgacgcg cccatcaacc ggcccaggat gttggcgccg
     2581 cgcgccttcg cgtgctcctc ggtctcgatg acgatcagcg cacccgcctc gccgaacacg
     2641 aacccgttgc ggtccttgtc gaaaggacga cacgcaccgg ccgggtcgtc gttggtggtc
     2701 gacaacacga tgcgcatctg agcgaacgcg gcgatcggca ccgcctcgat cttcgattcg
     2761 acgccgccgc agatagcgac gtcggcctcg ccgagcacga ggttgcgcca cgcctgggcg
     2821 ataccctcgg accctgaggc acaggccgac accggcgtgc tgacgccggc ccgggccttg
     2881 cgctcgagcc ctactgcggc cgccgccgcg ttgggcatgt acttctgcac ggccagcggt
     2941 gacaccgccc gcatgccccg cgcgcgcatg tcgtcgtagc tgaagaccag ctcttcggtc
     3001 gagcccatgc cggtgccgat cgacaccatc agacgtcgcg gatccacctc cggcgacccg
     3061 gcgttctgcc acacccgccg gctcagcacc gtggacatct tctgcaggta cgacagccgg
     3121 tgaagctcga ccttcgtcag ctcgggatcg aaatcctcca ggagatgccc gccgatgcga
     3181 accggcaggg agtactgctc gacgaacggg tcctccagct tgcggatgcc gctttgtccg
     3241 tccagcagct tcttccaggt ggtgtcggcg tcggttgcca aggcagtcgt catcgcaatg
     3301 ccggtgacga ccacgttggg gaatccattc cccgtagaca gccgtgtcat tgcttctccg
     3361 acaaatcctt tcgcatcctt ttcgcggctt tcaatacttc ccgaacgcga gagcgacgtt
     3421 gtggccaccg aacccgaacg agttgttgat ggcgtacttg tagtcgccgt agcgcggttc
     3481 acccgcgacg acatcgaggt cgatctcggg gtcgggggtt tcgtaattca acgtcggggg
     3541 gatcaccccg tcacgcaacg tcaagaccgt caagaccgac tctagtgcac cgaccgctcc
     3601 aatggagtgg ccgagcgcgg acttcggcgc atacaccggg gcgtgctcgc agcccgccac
     3661 gcgaatagcg ttggcctccg cggtgtcacc gatcggcgtg gccgtcgcgt gcgcgttgat
     3721 gtggtcgatg tccttggcgg acaggccggc cagctcgaga gcacgctgca tcgccttgcc
     3781 ggcacgttcg ccgtccggcg cgggcgccac catgtggaac gcgtccgagg tgatgccggc
     3841 acccagcagc cgggccagcg gcttggcgcc gcgagccttc gcgtgctcct cggtctcgat
     3901 gaccatcaga gcaccggcct cgccgaacac gaacccgtcg cggttcttgt cgaacggtcg
     3961 cgacgcgcct tccggatcgt cgttcttcgt cgacatcgcg cgcatcatcg agaacgccgc
     4021 gatcggcagc gcctcgatgc cgccctcgac accgccacag acggcgatgt cggcgtcgcc
     4081 catcacgatc tgccgccacg cgtgcgcgat ggcctcagag cctgacgagc aggccgacac
     4141 cggcgtgatg acaccggcgc gggcgccgag ctgcagcccg accactgccg cggcgccgtt
     4201 cggcatgatc atctgaaccg ccagcggcga caccttgcgg gggccgcctt cgttcatcag
     4261 gtcataggtc tcgacgatct tctcgccacc gccgagcccg gtgccgacca cgaccgcgaa
     4321 ccggtcaccg tcgacctcgg gcgtgccggc ggactcccag agcctgccgc tgatcagctt
     4381 ggccatccgc tggacatacg acatgcgccg caggtccagc cggttcatga ggtcgtccac
     4441 cggctctttg aggtggccac cgatgcggac cggcaggtcc catttggtga cgaactcgtc
     4501 ctcgagcacg cggatgccgc tctcaccggc caacaaaccc ttccacgtgc tctcgatgtc
     4561 cggcgaaatc gatgtcgtcg ccgtgacggc ggtgaccacc acgctgggga aaccgccgtt
     4621 agcagtggaa ggtttggtca tgtgcggtcc gcttcgatct tcgcgcggat ggcctcggcg
     4681 gcctcagggt tctcttcctc gagcttctgg atgtagccga ccacgtcacc aacggtgcgc
     4741 agcccggcca gatcctcgtc ggggatcttc acgccgtact tgtcctcggt ctgcaccgcg
     4801 atctcaacca tcgacagcga gtcgatgtcc aggtcgtcga cgaacgactt ctcgggggtg
     4861 acctccgacg gctcgatacc ggtgacctct tcgatgatct cggcgagacc ggcgatgatt
     4921 tcttcctgag tggcgggcac tatggctcct tctaattgat ggtgctactg ctactgatac
     4981 gtgcgttatg tggttgtcct ggcaaggcgc cggctagaag gccgccagcc cgtccaggtc
     5041 tgcgggcgac ttgacggcat gcgtgccgat cccccgaagt tcgcgtttgg cgatgccggt
     5101 gagcgttccc gcgggcggga actcgacgat cgccgtgaca ttgcgctgac gcagggtctc
     5161 ggtgcacagg tcccaccgga ctgggcgggt catctgggcc accagcttgg tcatcgcgtc
     5221 ttctgcggag gctaccggct ggccatcggc gttcgacagt acaacggcgg tcggctcggc
     5281 ggtctgcaca ttcgccgccg cggcggtgta ggcatccagc gccgacgaca tgaaatgcgt
     5341 gtggaacgcg ccggcggtgg ccagcagacg gacccgcgcc ttctcgggcg ggttctcggc
     5401 gagcttcgcc agcgcgtcga ggcggccggc ggccacgacc tggcccgcgg cgttgcggtt
     5461 ggccgggacg agttcgagct cgtccagacg ggccagcacc tcggtttcct cgccgccgag
     5521 caccgccgac atgccagtgg gctccagcgc gcaggccttc gccatctcca caccacgggt
     5581 ggcagccagc gcgacggcgt cgtcggcgga gatgatgcca ccgatcgcgt aggcggcgat
     5641 ctcgccgacc gaatgcccgg ccaccaaaac ctctcgcgaa atctcgcgtc gtgccagcag
     5701 gttgcggcgc accagttctt gatgggcgag cagcgtcgcg gccacgacga gcggctgggt
     5761 taccgcggtg tcggtgatct cctcggccgt cgcagtcgtg cccagcgtga ccaggtccag
     5821 gccgctgacc tcacaccacg acgtcagcgt ctctgacgcg cccggcaact ccagccacgg
     5881 tgagagcatt ccgggggtct gcgaaccctg tccagggcag agcaaggcga tcacagtctt
     5941 ttgaggcacg gattaagaaa acactgtgaa agtggtttta cgcggtgtaa gagattatga
     6001 acctggtctt aggtttttgt ggaggttcca caaatcagca tgtgatgcga ctccatcgag
     6061 acgaaattgc aaccgtcaac cagcgcgatg cacgccacgc ggcggtgtga tctgcgcaac
     6121 agacggataa gccgaggtcg ggttagttgc ctgcgatgcg agccgaccca cggttgcggc
     6181 aacccgcagc acgtaggcgt cgcggggcgt ggtggggtcg cggccggtga agtcggtgat
     6241 ccgcttgagc cggtagcgca cggtgtttgg atgaacgaac aatttgcgcg cgcatgcctc
     6301 gatggctccg ccggagtcca gatatgcgtc cagggtctcg gtcagcgccg gtccggcgtc
     6361 ggccaggggc cgcatcacct ctgcgtcgag taccgcgatg gccgacgcat cgccgagcag
     6421 cgcgcgttcg ggcagcagct cgcgggccag caccggccgc ggagcgctgc tccagccggc
     6481 gacggcgttc atgccggcga tcgcctccgt ggcgctggtg tgcgccgcgg tgaaggtcgg
     6541 cgccgtcggc ccgatcacca ccggcccgtc ggcgaacacg tcgatgagct cgcgcagaaa
     6601 cttgtcggtg ggggccaacg ggccggacac gatcgcgatc agccaggttc cgtggacatc
     6661 cgacagcgcc gcccggccat gtcgctgggc gatctcgcgc acgtcgtcgc tggcaaagtc
     6721 gacgcgctcg gctcggggca ggccgacgac gacggtcgcg ggcgcagtgg tgtcccagtt
     6781 cagcgccgcg gcgcgcgact gcagctcggg accggcgtcg ccgcgcacca cggcgtcgac
     6841 gaggttggcc tccatgcggg agtcccagga gccgcgggcc tcggcggcgt ccgcgtaggc
     6901 cgacgccgcg gtgaacgcga gatcgcggct gtaacgcagg attcccgtcg tcagcgcggt
     6961 ggcctgctcc tcggaccggg ccagcatcgg cacgacttcc tcgaagaact ccatcgtgac
     7021 gcgaaccatg tcgacggtct gccgcagcgc gatgcgccgg gtcagatcct gcggcaccag
     7081 ctcgaaggcc tgcacggtgt agcccacatt gccgtgcggg ttgcccatcc actcgacgaa
     7141 gttgaccacc gccgcctgca cgaccaggtg cacgctggcc cgctgcgagg cttccaggtc
     7201 ggcgaagaac ggcaaccgct cctgcatggc cgcgaccgcc tcggtggcga accgcccgga
     7261 atactgcttg agcttgcgca gcagcgattc cgggacggtc tccagcagtt cgagcgtgga
     7321 cagcggcggg gccggccgat ccttgtcggg catatctaaa agctacgcca cttttctggt
     7381 gaatacccat aacatacccg ttgactctgc gcacagagct caagtcgcct ctcgcgccct
     7441 gtgcgcagag tgaacgcgga tcaggccaca cccgggtccg acgtctgctc cggcgccgcc
     7501 agcacgtcgt cgatctcgta gcggcgcgcg gcctcgacgg cgacggactt gtcgatctcg
     7561 ccgtcgcgcg ccagcccttc gagcaccccg accgcgatcg actcggcatc ggtgttgaag
     7621 aagcggcgcg cggcgggccg ggtgtcggag aacccgaagc cgtcggtgcc cagcgtgatg
     7681 tacgtgccgg gcacccacgg gcggatctgc tcgctcaccg cacgcatcca gtccgacacc
     7741 gccacgacgg gtccacgcgc gtcggccagc gcctgggtca catacggcac ccgcggcgag
     7801 tggtcagggt ggcgcagcct ttccttttct atctctacgc cctcacggtt gagctcgccc
     7861 cagctggtca tcgaccacac gtcggcggcg acgtcccagt ccgccgccag caggtcagcc
     7921 gcgcgcagcg cgtcgggcat cgacaccccg gaagccagga tctgtgccga gtactgccgc
     7981 ggctcggggg cgggccggta gcgataaatc ccgcgcagta ggccctcgac atcgaggtct
     8041 tcgggttcgg ccggctgcgg atacggctcg ttgtagatcg tcatgtagaa gtagacgttc
     8101 tccgggttct cgccgaacat ccgggccagg ccgctttcga cgatgtaggc gatctcgtac
     8161 gcgaacgccg gatcgtaggc cacgacagca ggattggtgg acgccagcag aaccgaatgg
     8221 ccgtcggcgt gctgcaagcc ctcgccggtc aaggtggtgc ggccggccgt cgcaccgagc
     8281 acgaatccgc gtgccatctg gtcggcggcg gcccagaacc cgtcgccggt gcgctggaac
     8341 ccgaacatcg agtagaagat gtagatcggg atcatcggtt cgttgtgcgt cgcatacgac
     8401 gtgcctgcgg cggtgaacgt cgcggtggaa ccggcctcgt tgatgccctc gtgcaggatc
     8461 tggccgatct cactttcctt gtaggccaac atcatctcgg cgtcgacgga ggtgtagagc
     8521 tggccgttgc gattgtagat cttcagcgac gggaaccagg agtccatgcc gaaggtgcgt
     8581 gcctcgtcgg ggatgatggg cacgatgcgt ggcccaatct ccttgtcgcg caacagttct
     8641 ttgaagacac gcaccgtcgc catcgtggtg gcgacctctt ggtggccgga gcccttcttg
     8701 accgacttgt agatgtcgcg cggcggaagc gtcaaagcct tggatttggt gcggcgttcg
     8761 ggcacgaatc cgccgagcgc gcgccgacga tccagcatgt accggatctc cgccgcgtcc
     8821 ggcccggggt ggtagtacgg cggaaggtag gggttctcgt cgagttgctt gtcggagacc
     8881 gggattcgct gtgcgtcgcg gaatcctttg aggtcttcca gcgcgagctt cttcatttgg
     8941 tgggtggcgt tgcggccctg gaagtgcttt cccagcgagt aacccttgat ggtatgggcg
     9001 aggatgaccg tgggctggcc cttgtgttcg gtggccgcgc ggtaggcggc atacaccttg
     9061 cggtagtcgt gaccgccgcg cttgagactc cagatctcgt cgtcggacat gttctgcacc
     9121 agagccttgg tccgcggatc gcggccgaag aagtgctcac ggacgtaggc gccgtcgttg
     9181 gcctcgtagg tctggtagtc gccgtcggga gtggtgttca tcaggttcac cagcgcaccg
     9241 tcgcggtcgg cgtgcagcaa cgcgtcccac tcgcggcccc agacgacctt gatcacgttc
     9301 cagccggcgc cgcggaagaa cgactcgagt tcctggatga tcttgccgtt gccgcgcacc
     9361 ggcccgtcga gtcgctgaag gttgcagttg atgacgaacg tcaggttgtc cagcgcctcc
     9421 agcgccgcga cgtgggcggc gccgcggctt tccggttcgt ccatctcgcc gtcgccgagg
     9481 aacgcccaca cgtgctggtc ggaggtgtcc ttgatgccgc ggtcgtgcag gtagtggttg
     9541 aaccgcgctt ggaagatcgc gttgagtggg ccgaggccca tcgacacggt cgggaactcc
     9601 cagaattccg gcatcagccg cggatgcgga tacgacggca ggccgccgcc ggggtggctg
     9661 tgctcttggc ggaagccgtc gagctggtcg gcggtcagcc ggccttccaa aaacgcgcgg
     9721 gcgtagatgc cgggggaagc gtggccctgg atgtacacct ggtcgccgcc gccgggttgg
     9781 ctcttgccgc ggaagaagtg gttgaacccg acctcgtaga gcgccgccga cgacgcgtac
     9841 gtcgagatgt gtccgcccac accgacgccg ggccgttgtg cgcggtgcac catgatcgcc
     9901 gcgttccacc ggatccagcg ccggtagcgc ctttccacct cctcgtcgcc ggggaaccac
     9961 ggctcgagtt cggtcgggat ggtgttgacg tagtccgtcg acgtcagggc ggggatcgcg
    10021 acgcgttgct cgcccgcgcg ttccagcagg cgcagcatca ggtagcgcgc ccgcgccggg
    10081 cccgagcggt ccagcacgtc gtcgaacgac tccagccact cgccggtctc ttccgggtcg
    10141 atgtcgggca gataggatgc cacgccttcc cgaatcactc ggactcgatc ggattcattt
    10201 gagccggttg agttttgggc caggtcgcga cgggcgaact cggtggtcaa cttccgctcc
    10261 tcatcaggcg gtgcatggat acctccatcg tccaccaatc gtgccccacg tgcaccggta
    10321 gccggtaacc tcatcggctg tgctgggggt cggcggggtg cggcgatggc ggatcgccgc
    10381 actggtggcc ggtctgatcg cggcggcagc gctggtggtg gtcggctgca ccagcatgac
    10441 cagcggcacg ccggccgtgg acaccaacga cgcgcgggac tatcggacgt gggtggtcga
    10501 ttcgctgtcg cagtccgcgg cggcgtccag tgagcgggag tcgacgcgac aggtgtcgct
    10561 gacggccgag gccgtcgcaa acgcgtgcga agtgctcagc aacacgagtg tggacgcggt
    10621 gaccgcgatc aacgcgtacg tcagcgccgt cgcggcccac aacgaggcag acgtggccgc
    10681 gaaggcgcag gcggcggtcg acacgctgaa ccgtggcgcg gacgcggtcg ccgccaccct
    10741 caattccgcg ctgctgcctc acatgcgcga tgcgttcacg gcctgggtgg acgccacccg
    10801 ggcggtcgcg aaggccatcg gcgagcgcta cggccagcag gaattcaatg cggaggtcga
    10861 caaattcaac caagcgaatg acgctgcgct gaatacatgc ggtggctcgc cctgagcgac
    10921 ccggccgctg gtgctagggc gcagcgccgt agcgtgcaac gatagggcag gacagggcgg
    10981 ctcacagaca agggaggttt cgacggtggt cgcggcgggt gacgccccga actacgcccg
    11041 aagactgggc atcgaaaaag atcaggttgt tcaggagttg ggctgggacg aggacaccga
    11101 cgacgacatc cgcgccgaca tcgaggacgc ttgcggaagc gagctgctcg acgacgactc
    11161 ggacgaggtc atcgacgtcg tgctgctgtg gtggcgcgac ggggacggcg atctggtcga
    11221 ccggttgatg gacgcgatca cgccgttgtc tgacgacggg gtcatctggg tgctgacgcc
    11281 gaagaccggc aaacccggtc atgtgctgcc ggccgagatc tccgagtcgg cgccgacggc
    11341 cgggttgatg cagacgtcgt cggcgaacct gggcgactgg agcgcaagcc ggctggtgca
    11401 accgaaatcc cgggcgggca ggcgctgatg ctggacgtcg gcacgtctgc accagacttc
    11461 acgctccgcg accagaacca acagccggtg tcgctgagcg acttccgtga taacaagagc
    11521 gtgctgctgg tgttcttccc gctcgcgttc accggtgtct gccagggcga gctcgacgag
    11581 atccgcgacc acctgcccga gtacgagaac gacgagacgc agacattggc gatctcggtg
    11641 ggaccgcctc caacgcacaa ggtttgggcg acggagagcg gcttcacctt tccggtgctg
    11701 tcggatttct ggccgcacgg tgcggtcgcg caggcttacg gggtgttcaa cgacgacgcg
    11761 gggttcgcga accggggcac gttcgtcgtg gataagtcgg gcatcatccg gttcgcggag
    11821 atgaagcagc cgggcgaggc gcgggaccag agtctgtgga agaccgcgct ggcagcgctg
    11881 cagtcctaac tagcggcggc cgcgatactg cgatttcgta tacgacgcgc cgtcgagggt
    11941 gtacgaatcc gcagattcgg gatttcccag agccactccg cagcccttat cctgccccag
    12001 aacgggcgcg tagctcagtg gtagagcact ggttttacac accagcggtc ggcggttcga
    12061 taccgtccgc gcccaccatt gtcagtgacg tgccgcctcc tgggcgagct gcacccgcga
    12121 ggtcagcccg agcttggtgt agatgtgagt gagatgcgcc tgcacggtgc gaggggagac
    12181 caacagccgg acggcgacgt ccttgttgcc gaggccttcg ctgaccaatc ggacgacgtc
    12241 gagttcggcg cgggtcaacg acgcccaacc gctgctggcg cgcctgcgtt cgccacgacc
    12301 acgctgtgcg taggcgatcg cctcctcgat cgacaacgcc gcgccttccg cccacgcggc
    12361 gtcgaattcg ttctggccca aggcatcccg aaccgtggcg atccttgcct catcgtcggc
    12421 gtcgagaacc ttgaagcgca ccattcccat gcggcggcgg gctgcttccg ccgcgccgaa
    12481 cagccgcgct gctagcaggt cgttgcctgc ttcacccgcc acaatggcga ggcagtcgag
    12541 agcgaacgga acgacgaggt cacctccgag acgggccgcc aagtcgagcg cgtcataggc
    12601 atcgcgttcg gcagcgccga gttcaccgcg cgcgatttcg acgagtgcgc gcgacgtcaa
    12661 ggccgccgcc agagcccagc ccgtgttcga tgacacggca tcgtcggccc agcgaccggc
    12721 cgcggcgaga tcaccgctcg tcaacggggc cagggccgcc caggtgaata tcgaggacgt
    12781 catgggatcc atcgcggtgc gctcgcgcgc tgtttcgtat gcctcccatg ccgcggacgc
    12841 gttgcccgcg gcaaggtgga caatgccgaa cgccgagtag acgatgccgc tgtatgaatc
    12901 aaagtcggat gcgcgttcga gggccacgtc ggccaccgct cgcgcacttg ccgcatcccc
    12961 caggtaagcc agcgcgtaag cacacgtcac cgatgcgcca atcgagaaca cgtcgtggga
    13021 ctcggccacc tcgcggagcc gagcgactgc ctcggtcagg tcgccgcgga ggacttgcgc
    13081 ccagcccagg acatagcgca actggcggga gacgaagccg tcgccgatgc ggtcggcgat
    13141 cctgattcct tcctcggcgg ccacctgcgc cgcgacgggc tcgttccaca cgcgcgcggt
    13201 catccactct ctaggccgaa gatctgcccc aaccaccggg aatcgccgag gtcccgcgca
    13261 agctccgctg cctcggcgaa gtatgtcccg gccagctcgc ggtcgtgggc gttggcgagc
    13321 gcaccgcagg cggtcagcgc ccgcgccacg agcgccgggt caccaagttc ccgtgccgct
    13381 gccagagttc gctcggcgtc gtcgagtccg atggacatgc ccctaaatga catcagcagg
    13441 gctttgtccg cagacgcccg cacacgcgcc gcggatgtgt cggggctctc tggaccttta
    13501 tcggcaaggg cggcaccgag ccagttcagc ccttcctggt ggcgtccacg ggtcagccag
    13561 agcggttgca gcgccgatgc cagctccagc gccaggtcgg tgtccgaact ttctcgactc
    13621 cacgcgaaag cggcacgcag attgtcgatt tcggtttccg cccgctcgag gcggcgttcg
    13681 tggtcggcct ctgccggcgc gtcgagcagg gccgccatcg cggtgtagtg gtcgcggtga
    13741 cgggcccgga cgtcgtcacc ttcgtgggac tcgccgaggt ttttcctgtg cgtactgacg
    13801 gacggtttcg agcagccggt agcggggtcc atgcgggctg tcgtcggcga ccacgagcga
    13861 cttgtcgacc agcagggtga gctgatcgag tatctggtag cgctccactt cgccgccgcc
    13921 ggcgacagcc tgggcggcgt cgagatcgaa cccgccgagg aacaccgcaa gccttcggaa
    13981 caacacctgt tcgggctcag acagcattga gtgcgaccaa tccaccgatg cgcgcaatgt
    14041 ctgctggcgg cgcaccgccg tacgggcacc tccggtcaga aggcgaaacg cgtcgtgcag
    14101 actgtcgacg atttcggcgg gggacaatgc acggacccgg gctgcggcaa gttcgatggc
    14161 cagcggaagg ccgtcgagcc tgcggcagat ctccgtcaca atttcggcgt tgtccgaggt
    14221 gacacggaat tcgggcttgg tcctgcgtgc gcgtgcgctg aacagctcga tcgctttcgt
    14281 cggccagtga gagggagggc acccgccagc tcacctcacc ggccacgccg atcggctcgc
    14341 ggctcgtcgt cagcaatgtc aacgtcgggc atgcgcctag caggcccgta atcagctcgg
    14401 cgctggcgtc gagcaggtgt tcgcagttgt cgagcaggag cagcatctgg cgatcgcgaa
    14461 tgaaccgcag cagtacgtcc atcgtcggtt ggccgggttg gtcgggcagg cccagcgcgc
    14521 gcaccgccaa ccgcgtcttg cccgcgccgc ccgcgccgcc cgcgtgacga gccggttgtc
    14581 ggcgagcgcc tcacggatgc cattcatttc cgcttggcgc ccaatgaaac tcgtcaattg
    14641 cgccggaaga cgctcggcgg caacggcatt ggcagtgcgc agcggcggga agtcgttgtg
    14701 cagatctggg tgacagagtt gcaccacccg ttcggggcgg ggcagatcgc gcagcggatg
    14761 ggagcccagg tcggtgagcg agacgtctgg cgggagttgg tcgatgacaa gcggttccgt
    14821 cgcgccggac aacacggtct gaccgccgtg ggccaggtcg cgcagccggg cggtgcggtt
    14881 gatcgtcggg ccgacgtagt tggcctcgtc gcgcagttgt atgtcgccgg tgtggacgcc
    14941 gatgcgcaac ttgatcggtg ccaacggtgc ccgctgcaag tccagcgcac aggccaccgc
    15001 gtcggcggcg cgcgcgaacg cgatcacgaa gctgtcgccc tcgccctgtt ccacgggtcg
    15061 cacaccctgg tgggtggcga ccgcgtcaga cagtgtctgg tcgaggcgcg cgacggcggc
    15121 cttcatcgcc tcgggctggg tctcccacaa ccgggtcgaa ccctcgacgt cggccagcag
    15181 caacgtcacg gttcccgtcg gaagctcgct catgtccacg tcgctagttt cgcccatgct
    15241 agccagcatg cggcgacgtg cagcgcagaa catcagcgaa atcgccaata gttgcggcgt
    15301 agggatgcgt acgtacgcgc ccgactatcg actagccacc agcaggtggc tttgcgtcgc
    15361 cctccttatt cagcgcctcg ctcgcgctgc cgacgacgct tcggatgaac tcgtactggg
    15421 tcttgacgag cttgtccgcc atgtccaggg cggcgtcgac gatggtcttg cgcctcgacg
    15481 ggccgtcgtc gccgtgcccg ggaatcgact catcgacggt gccgacgaac ttgcgcacgg
    15541 catcgatcgc ggcccgctgt ccggattcga ccgacttgag cacgtcgtcg gacaggtctg
    15601 ccgctcgtcc ggtcaggtct gctgctcgtt ccgcgacggg ttttttctcg gtgctgttct
    15661 ctgccatggc atcagaatgc gctaagggcc cggcgtcctg tagggccgat ggtcccttgc
    15721 gttgccggtc ggcagcgact agtcagcgcg cttcgcgtcg tcttccttgc tcgcggtctc
    15781 gctcccgccg cgcacgaagc tgcgtaagaa cccgtacaag tccttcgcct cgcccggctt
    15841 gctcaccatc ccgctcgcgc cgcgcacggc gctgcgcaag tactcgttcg ttgtcgtggt
    15901 gagctgctcg gcgtaatccc cgatggcacc gacgaccttt ttacgcgccg acgggtcgtc
    15961 gacgaggtcg ggcgtcgcgt cgtcgaggcg gtccacaagt ttgcgtatag cctcgatcgc
    16021 cgccagctgt ctggcttcga tccgcttcag cacctcgtcg gacaggtccg atgcacgttt
    16081 cgccgcggtc ttcttcttgg tgctctctgc catgagcgcg ctcctttgaa tgcagcagtg
    16141 aggcttggcc ttagaatgcg ccctaacccg ccgttctaaa cagggccgtt ggtccttgag
    16201 gatccggcgc gggctcgttg tcggctcgac tccctccgcg cccgctcgct agctcagccg
    16261 aagttcgagt cgttgtgatt gggacagtag gcggcgacgg ccgaacttgc gatcccgcgt
    16321 gcttccgttg ggttgaagac tcccttggcg acgatgttgt tgaccgcaga cacgaatgtc
    16381 gaatggccag ggctcctgtc gagggccgag cagaccgact ggccaaggga aatcatctcc
    16441 tcaaaagagg cacggccctg atagttgacc aggtttccac tcagcgcctt cgagtaccaa
    16501 tcgtcggcgg tgggttccgc ctgtgcgacc gggcacatgg tcagtgccag tgcggtcagc
    16561 gagacagcga atgttgcgac gcggctcatc ttcagcctcc ttacgaggaa ggaaggcgac
    16621 gatactccag gaccgcaatt tgcggatcag tttcgccagg tcagcgattg gtcggcgtta
    16681 gccggaaaac cgggatcgtc cggccgatag gcgaggcgcc gtcttcgcgc cggtcatctt
    16741 cagcgtcgcc gacggcacgc tggacatgcc ccaactcact cgtccgttgc tgattcgccc
    16801 gagccatgga tccgttttgg acgcaacgtg tttggcgatg tactgtcccg cctgcgagcg
    16861 cccgaacctg ttcaacgcac gcatcacagc cgtcgtggga cggttcggat cgacgtgtcg
    16921 cagcggcatg tgttcgaccc cagaccagct gcgttatggg ttgtagtcgg tgctgccctc
    16981 gacgggatgc gtcgtgctga tcagcttggc gaagcagtgg tttcgatcga aaccccgggc
    17041 cgcgcaccac gccttcgcat cgcgggagtc ggtgtaagag atgccgacca ccgtcaccca
    17101 ccattgatac ttggtgtcga acgtcgacca gtcaggtgag tacaagagcc tgacgcccgg
    17161 atagatctgc cgcaactgaa gatgttcctg caggatcttg gcgtagtccc aggtcgtgcc
    17221 ctctgcaacg agtccgtagt acttggaact gatttgggcc acccagcgct ctgcgaggta
    17281 aagggacacc gaagaccggt cgccatcggc gatcgcccgg agccggttca gcgcggctgt
    17341 ctcggaatcc accgttgtcg gtgttggccg aggcgacgac gtcgtggtcg gcgtgcgcgt
    17401 ggtcgtcggc gtgggcgtcg ccgacgaact cggttctgaa ctggcgaacg aaatctcagc
    17461 cccggcacgg gcttccgcct cggtgtacgt cgactcgcgt tggccggcca cctgcacccg
    17521 gtagaacttt gccccgcccg gcacatcgct gatgctgaag cgcaggttgc aggagtcgcg
    17581 caccaggtag ctgctgtcga aagtgccctt cgccaacagc tttcgcgcct cgtccgcgac
    17641 gatcaccggt gcgccgggtc cgatgtctgc aaagctttgc gcaccggcgc acggaaatcc
    17701 cgacggcagc cctaaggtcg tcgtgctgtt gcccatcaga tgcacggtgc ctgccagcgt
    17761 gaacgcctca gccggcgtgg gctagctcac ggctttgaag ccgatgtaag ccgcgacgca
    17821 caggacgtac gaatgctgcg gctgcggcca gcgcgatccg agaacgcgcg gtgtttgacg
    17881 cgccatgttg cacaaactga ggcatcgtcg gagcgctcac cggcggtgtc ggccgccaag
    17941 ttgctgaact gccaagcgcg cgtgcgaatc catgcacgat acgaacctgg ccgtgggatc
    18001 cttggccagc gccttggcca gcgccgcgtc gagtggggcc agctcgggat gccggtcagt
    18061 caacgggggc ggttgggcgt tcagatgctt cccgatcacg accgccgcat tggagtgcgg
    18121 aaacagcggc gcgccggtca gcaggtggta agcggtcgcc gctaacgcgt actggtcagc
    18181 ccggccgtct aatggccgac ccatcaactg ctcgggagcg gcatacgcca ccgttcccac
    18241 ggccatgttg gtaagggtca ggccgctgct gtccccgagg tctcgtgcga cgccgaagtc
    18301 gctgagaaga atgcgccgct cgtcgtcgtc cagccgagcg atcaagatgt tcgagggctt
    18361 gacgtcgcgg tgcaggagac cccgtgcatg ggcatagtcg agcgccgacg cgacggccgt
    18421 gatgatctcg atcaccgccg gcaacggcat cccggtcgga tactgctccc gcagcaagcg
    18481 gccggcgtcg gtgccgtcga cgtagtccat cgaaatccac agccggtcct cgtcgtcgcc
    18541 gcggtcgtgg attcccacga tgtgcggatg ccacaacttg gcggccagct ccgcttcccg
    18601 ggtgaacctc tcccggaagt cggagttcag cggagatgtc ggcccgcagg actttgaccg
    18661 catcctgccg aggcagtcgc ggatgctgga cgaggtacac ctcgcccatc cccccagcta
    18721 cgtggcgtcg accgtcacca ccatcgtgta gggcggctcg atcttttctt tgtcggtggt
    18781 gctccacgcc ttcatgaagg tgtcgatgga gaacgtggta tcgccgtgcg tgtccaaact
    18841 gtcgttgagg tgcacgacgc cggcggtggt gtcgacgccg gtgaccacca cgacgtggtt
    18901 ggtgtaggtg ggcttgccct ggccatacag tgcgcccgaa tcgaggtgga caagcacctt
    18961 gtgccgctgg caaggtagcg ctcaagcgcc tccaagccgg ttgggatgcc gtcggaggtg
    19021 gcggcgatct cgacgtcggt caccacggcg tcgatcccgt aatgcgcgag cagtaccacc
    19081 tcgtcgggag ccttgctgcc gctggccgtc gtgatgtcgt tggggtcggt cgggggagtc
    19141 cagacgggct gaccgggccg tacgacgctg ggggtctgct cggcgagcgc gatgatctgc
    19201 tgctcggtgg gtgacttgcc ggtgagctgg ccgacgatgt cggcgacggc catcagcccg
    19261 cagtcgcccg tgtgctgcgg gtgccagtac tcagctgctt cagtcgggtc gccgtagagc
    19321 ctgccgtcgg agttcggcgc ggcgtgggcc gatgcagcca gcagaactgc agcggccaac
    19381 agcttgccga agattatgag gatgaagccg acgagggcga tcatcacgcc gaccttgcgc
    19441 ctgcgggtgg acttggtgtt cacgagaaga ggctccggcg caggtctgct gagatcctca
    19501 acgacttctt ggcgcgcggt caggactgct gcgagcgcga ctactccgac ggcggggcga
    19561 agaactccag cgcgatgcca tcggggtccc ggaaactcag cccggatccg tagccggcgt
    19621 cgacgatgcc gccgtggctg atcccgagtt cgttgagccg gttcatccac ttctccagct
    19681 caccccggtt cgcgcacgcg aatcccacat gatccagtcc gacccggaac tcgctgaagt
    19741 gctcgtcggg cactccgcgg tcgtgctgat gaatgcccag cagcgtgccg ccgtcgagca
    19801 gccacacgag atgacggaat ccggcgtcgg tgtgctcgtc gagcaccggg tcagcgccga
    19861 gcagccgtcg ataccacgga ccactgacct cgatgtcttt cacggtgaga gccacgtggg
    19921 cgaggccggg gaagtcgggc atggatgcag actagacgat gcgacgaccg cgtcgggttc
    19981 gagaccgcat gtcccgcagg tacacctggt agcccagcag ccagagctgt cgcgggatca
    20041 ctcgatccgc cagccgcaga cccgtgagca acagctcgaa gcggcgctgc tggcccgacg
    20101 tccacggcag ctgcatgtgc gttcggaact ccgcgggcag aaagcccgtc gtcgcgaaca
    20161 gattcagcgg accgccgatc acccgcagcg gccacggcag aaacgccaag ctggcgaccc
    20221 cgtagaggtg ttgccgcact ggcggatcga tcctcaactc gtccagtgaa cgcttccagt
    20281 actcgtcgaa cgcgatgcgg tccgagggcc acatgctctc cgggacttgc agcgtggtgc
    20341 cgagccgttt cgcgtcgcgg tacaccgcgt cggcgcaatc gtcgtccagg ggtccgtaca
    20401 ggaactcgtg ctggtcgacg aagtagcggt acaggcaagc ggccacccag agctgaagcc
    20461 tagggtcaaa tgcgttgtag gacaacgggc ttcgggcggt cgagcgaacc tgcgcgtgga
    20521 cggtgtcgac ggcgcgccga atcgactcgc ggtccttgtc ggtgcctacc gtggccgccg
    20581 ccaggtacgt gcccgtcgtc cgtgcccgct tgaacgggtg cttgtacacg ttgccggagt
    20641 cgacgggact ttccagcact ccgtatccga cggccggcgc cgacagctgc atgatcacgt
    20701 tggccgccgg gagcagcagc cccgccgggt tcaacaaatc cgcgactcga ttcggacgcc
    20761 gcatcatgtc agcagtacac cacgatgtga gacgctgccg aggtgtttgg gcgcagggcc
    20821 gtcgggctgg ccgccgggtg gctggcggac ttggcgttcg ccgacccgcc gcagcaccat
    20881 ccggtcgccg ggttcggcgc ggccgccggt gcgttggagc gcaggacgta ccgtgacgtg
    20941 cgctgggccg gagtcgttca cgccggcttg ctgctgagcg ctgttgggct tctgggcgtg
    21001 gcggtcgagc gtgttgccgg ccggatcggc atcgccgccg cgacgtggac ggcgttgggc
    21061 ggcacgtcgc tgcttcgaac ggtcgcggcg atgacggaca cgttgtctgc gggcgacgtc
    21121 ggtgcggcca ggcggctgct gccgtcgctg tgcggccgcg atcccgccgc gctcgacgcc
    21181 gacgggctga cccgcgcggc attggagtcg ttggccgaga acacatctga cgcacaggtt
    21241 gccccgctgt tgtggacggc ggtcggcggg gccccgggag cgctggtcta ccgggcggtc
    21301 aacacgctcg atgcgatggt cggctaccgg tcaccgaggt atgcccgatt tggttgggcc
    21361 gcagcgcgat tggacgactt cgccaacttc ataccggccc ggttcaccgc ggcgctggtg
    21421 gtcatgtgcg ctccgctcgt cgggggatcc ccgacgggtg cggtccgggc gtggcgccgc
    21481 gacgcgaaac ggcatccgag tcccaacgcc ggggtggtcg aggcggcgtt cgcaggcgcg
    21541 ctcgggattc gcgtcggcgg acctacccgc tatccggatg gactcgagat tcgtccgact
    21601 cttggtgatg ggccggcgcc gcggatcgat gatctgcgcc gcgcggtcgg gctgtcacgg
    21661 gcagtacagg cggcagccgc ggtcgtggca gtcgcctgcg tcagcttcgc cagtcacacc
    21721 agtaccgcca gacctcaatg gatatctctc aaaaagcgat agcgcatgcg atatcgctcg
    21781 tgaggacacg ggttgtgaca tccttttcgc gtgtccgcgc cgccttcagg tgttgtgacg
    21841 tttctgttca ccgatgtcga gggttcgacc cgtcggtggg aggcggacgc ggacgggatg
    21901 cgggcggcgc tggccgcgca tgacgaggtg ttgcgcgggg cgatcgaggc gcatggcgga
    21961 ttcatgttca agcacaccgg tgacggggtg tgcgccgcat tcgcctcacc gaagtcggcg
    22021 gtcgacgctg cggtggctgc acaagggcag ctggccctgc cggtgcggat ggggctcgcg
    22081 accggcgaag ccgagctgca ggagggtgat tatttcggcg cggtgctcaa ccgcgcggcg
    22141 cgggtgatgg ccgccgggca cggcggtcag atcctgctgg ccgagtcgac ggcggctctg
    22201 ctcagcggca tcgatctgat ctatctggga ccgcgccggt tgcgcgacct gcccggcgca
    22261 attggcgtgt accaggtgcg agcacccggc ctgcaaaagg actttccgcc attgcgcgca
    22321 ctcgacgcca gcccggggaa cctgcggcct gcggtcacga gcttcgtcgg ccgtgaggcc
    22381 gaggtcgccg aggtggaagc ggcggtgcgg gcgcatcggt tggtgacgtt gaccggggtc
    22441 ggcggagtcg gcaagacccg cctggcaacg gaagtcgcgg cgcaggtggc cgacgaattt
    22501 ctcgacggcg tctggttttt cgagctggcc gcggtggccg atccgtcggc cgtgcccgac
    22561 gcggtggccg cggtgctggg catcacccaa caaccgggca agagtgtgag cgagagcgtg
    22621 gcggccgcgt tggagggccg agtgcggttg ttggtcttcg acaactgcga gcacgtcctc
    22681 gacgccgcag ccgatctggc cgaggccatc ttcacccact ccgcaatagt cagaatcctc
    22741 gccaccagcc gcgaggggct gggagtggct gacgagcaag tgtggccggt gccgtcgttg
    22801 gacgtcggcg cgggaaccga ctgtgccgcg gtcgaattgc tcgtccaacg cgcccgtgcc
    22861 gtggcgccgc gcttctcgat ggccgagcgt gacgaggcgg cggccgtggt ggagatctgc
    22921 cggcgcctcg acgggatccc gttggccatc gagctggccg cctcgcgtat ggcgtcgatg
    22981 accgcgagcg aggtgcgtga ccgtctggat caccggtttc ggctgttggt cggctcccgt
    23041 cgaggactgg cgcgccacca cacacttcgc cacgcggtcg ggtggtccta cgacctgctc
    23101 cacgacgccg aaaaggcgct gctcgaccga tgttcagtat tcgcgggcgg cttcaacctc
    23161 gaaagtgcct gtgcagtagc aggatccgac gacgaatttg ccaccatgga tctgctcgac
    23221 gcgctggtgc gcaagtcgct ccttactgcg gaccggtcag ccggccggac gaggttctcg
    23281 atgctggaga cgatccggca gttcgccgag gagcaactcg tcgttcgcgg cgaggcgacc
    23341 gaggcgcgtt atgcgcatgc tcgccacttt gcgctgcgcg aagccgacat catggccctg
    23401 tgggacagcc cccgccagcg ggaggcgtac gactggtttg ccgccgagct ggccaatctg
    23461 cgcactgcgt tccggtgggc cgccgaccag ggtgatctcg acgtggccgc cgccatcgcc
    23521 acatacgcgg gattccttgg ctacctggtc gagaactatg aaccgatcac ctgggccgaa
    23581 gagctgatcg agcccgcccg cgctgtcgac caccctcggc ttgtgttcct gtacgtgatg
    23641 gcgtcgctgt gctggatcgt gggacggatc gaggaggccg tccagcacag cgatgacggg
    23701 caggtggcga tgcgcagtgg ccgcggcgag gttccgttcg gcttcgagac cctgctgggt
    23761 agcgcgtatc tagccatagg ccaggccgat cggtgtgtcg agtggtgccg cgcccaaact
    23821 gcaagcagtc gcgacgcaca cgcactcggc agggcatccc tggctatcgc attgacgctg
    23881 gccggtcgcg gtgacgaggc gatggccgcc gcggacggcc tagtcgagac tgccgaggcc
    23941 acccacaacc cctatgcgct ctcccttgcg ctgatggcct acgcattcgc ctgccgcgac
    24001 gcctaccccg accgcgctct cgaagcgatg caccggggcc tggtgatcgc tcaagacagc
    24061 ggcaaccgct tcaacgaaac ccacctggcg gccactctgg ccgaacttga agtcgaacgc
    24121 gacgatccgt tggcggcgct cgatcacatc actctggcga tccgcaattt gcacgattcg
    24181 gacaacgtcg tggtcatgcg ttcgcccttg acaaatctcg caat
//
LOCUS       DASWBF010000227        13268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_349677,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000227 DASWBF010000000
VERSION     DASWBF010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13268
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349677"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VF874_08060"
     CDS             complement(<1..142)
                     /locus_tag="VF874_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9176036.1"
                     /translation="MKAVTSTEAKAKLNALLAEVERTGVSVTITNHGRPVAVLTPARQ
                     LPR"
     gene            complement(221..1420)
                     /locus_tag="VF874_08065"
     CDS             complement(221..1420)
                     /locus_tag="VF874_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176037.1"
                     /translation="MQWSSDLLGDAEKSLLSRCSVFAGGFDLPAAHAVAGSDDEFETL
                     DLLDALVRKSLLVADRSAEQRRFSMLETIRQFGEEQLTATGEAKAARTAHARHFAERE
                     ADVLALWDSPRQREASRHRGRDRQCHVTGRIDDAVGYLDAGHAAIESGRFDDVRREFE
                     GILAGAYLANGQLERCVEVYRNIVARSPGGACTVARSSLALALTIAGAGDEAMAVSEG
                     LIATDATDNPHVVSWALFAYGFARGDADPELAYDVLRRGLRIAQESGNRRVESILTVG
                     LSRLAATHADPTNAFHFLSVAIRNLYDSGSFSILPTPLAVLAAIFDRLGHHEPAATIC
                     GFAATPLSRTAFPEINRAITHLRELLGDEVYESFARASEHMTNATMATYALDQIDQAR
                     AGLLRAH"
     gene            complement(1445..2104)
                     /locus_tag="VF874_08070"
     CDS             complement(1445..2104)
                     /locus_tag="VF874_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEX9176038.1"
                     /translation="MNELVEVQEALKSHRLMTLTGAGGVGKTRLALEVADRLADNFPD
                     GVWVIELATVADPAAVPEAVAAVLGITQQPNMSLADSVAAAMEGRTRLLVFDNCEHVL
                     EAAAELTEAILSQSAAVQVLATSREGLRVADEQLWSVPSLDIGSGAAMLFAERAAAVA
                     PGISLDDDAGAVVEICRRLDGMPLAIELAASRMQSMTIKELQHRLDDRFRLLVGSRRG
                     L"
     gene            complement(2270..2683)
                     /locus_tag="VF874_08075"
     CDS             complement(2270..2683)
                     /locus_tag="VF874_08075"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9176039.1"
                     /translation="MKRVQKARTAPSGVVTFLFTDIEGSTRRWEADADAMRPALETHD
                     RVLREAIEAHEGWLFKHTGDGVCAVFASPRAAVDPAVMAQLKLELPVRMGISTGEAEL
                     RGRDDFGRAQPRGARDGGGPWRADPARRRHRGPAH"
     gene            complement(2784..3641)
                     /gene="sigI"
                     /locus_tag="VF874_08080"
     CDS             complement(2784..3641)
                     /gene="sigI"
                     /locus_tag="VF874_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigI"
                     /protein_id="HEX9176040.1"
                     /translation="MTDAQLQEAWRRHHPYVVNLTYQMLGDIGDAEDVAQEAFARLSG
                     VDGIDDVRGWLTVVAGRLCLDLVRSARHRYESATDTTILEEALAHGIVDPADRVTLDD
                     QVGDALLQVLRRLSPGERVTFVLHDVFKVPFVEIAETVGRPVGTCRQLARRARAKIEA
                     SGPATTDVTRDEHRAVIEKFISACATGDLAALTAVLDPTVWGAATLLGDPAPPPQVNH
                     GPDAVATNLMRYLGHGATLVSAPVGQPVLLGFAHRQLFAVLVLTVRNGLVAKIEATAD
                     PSAFSRGNG"
     gene            3745..4581
                     /locus_tag="VF874_08085"
     CDS             3745..4581
                     /locus_tag="VF874_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX9176041.1"
                     /translation="MPIVVTGATGNVGRPLVAELVAAGVEVRAVSRRPDTAAFPGEVK
                     VVDAAAAAIPGASAVFLNSRALGDDMESVVRLAQRHAVTKLVALSAINADDDFSRQPS
                     RYRGDRNKEIEQLAVASGLPWVSLRPSVFATNFAGMWAGQIRAGDVVNGPYAAASSAP
                     IVERDIAAVAARALLSDELVGQRIPLTGPQALTNTEMVDIIGSVLHRPLRYSEIPAEL
                     VRQRFIALGFGTEFADAYIAMLATTVDRPALVTHDLQRILGRPAETFGQWVADHVSLF
                     TN"
     gene            4597..5043
                     /locus_tag="VF874_08090"
     CDS             4597..5043
                     /locus_tag="VF874_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9176042.1"
                     /translation="MSNPQPPRYLKPMNKIMMAIQKLGIPTGPAMVLTVPGRRSGQPR
                     STPMTPFTHDGKLYTVAGYPGADWPRNARAAGEGTLSRGRKSRRVKIVELSADEAGPV
                     LREFPGQVPVGVGFAKRSGLVVDGTPDEFEALAGQLAVFRFDPLTF"
     gene            complement(5040..5492)
                     /locus_tag="VF874_08095"
     CDS             complement(5040..5492)
                     /locus_tag="VF874_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014584749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176043.1"
                     /translation="MRSFEIASDLAASPEAVWQRVVTPAGINDELLPLMRMTMPRHLR
                     GATIDQIPLRQRIGRCWLLLLGLVPVDYDDLALVEVDPGRRFLERSTMLTQSQWSHER
                     IVEPQDGGCRVIDRLHWQGRTRAFEAVFGAAVPILFRHRHRRLRRHFA"
     gene            complement(5497..7227)
                     /locus_tag="VF874_08100"
     CDS             complement(5497..7227)
                     /locus_tag="VF874_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HEX9176044.1"
                     /translation="MCAQKNSEPNSGRAEAVLTWLGGGHWRELGERHERSTAAIAGVV
                     VLVGAALTWLVATVAVAESTQWPVWAILPLTLLFGLLVGAVSRAAASGPTRGWAVAGR
                     GAVAALVGLVVGELAAVVLFSGSIDRRIDEDAARSASATPAVAQAEANLGRMRDTRTA
                     LDTAVEQARAHRDQALVVARCEFNPTPGCPQTRITGVPGSGPETRSANQILRDTQREL
                     DNAVAVRDQQTPALNAQIAGAEQGVTQSREAAVAAADRGLGARWVAMHAVTLAGAGAL
                     VVWAVTVAFFALLSLLPLILKLLRGETSHDRGAAARAQRDRAELNADTAIAVKRAEVR
                     AAIETIWAEQQLASARLAVEAQHEIDRELHRRRVVEALDDAPVQATLQRPPPQVGGGT
                     SRMRGSTPTEPGEEDMYLPIAAEAEAASRGALPAADNLPARVEDERTPARPTIPNVTK
                     TAARWIRPLVPPIVARAIDTTTHPLRTARQVFEEVEEITFSFKRTHKVTVFDEETPEH
                     SQQRGPAASDDARRVESSRPEESESLAWAAADEAYGLSARERQPEIGQWDGPARLRGR
                     GNPRELPSAE"
     gene            7376..8929
                     /gene="fadD4"
                     /locus_tag="VF874_08105"
     CDS             7376..8929
                     /gene="fadD4"
                     /locus_tag="VF874_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD4"
                     /protein_id="HEX9176045.1"
                     /translation="MQIREHVSTKPAVILHPTGTVVTFAELEARANRLSHHFRRAGLR
                     EGDTVAAVLENNEHVHAVMWAARRCGLYYALINTHLTAAEAAYIVENSGAKAVIGSRA
                     TRPICEGLASLTELPELLLIADGAAGSATSASRDLDGWQRYPECVADEPDTPIDDERE
                     GDLLQYSSGTTGRPKGIRRPLPHVTPAEAPNLLSVLLMAIGMTPEAVYLSPAPLYHTA
                     PCVWSTSALAMGATVVVMEKFDPEQTLEAIQRYRVTHGQFVPAMFVRMLKLPEAVRNS
                     YDVSSLKRVVHAAAPCPVEIKQQMIDWWGPIVDEYYSSSEGAGITFITADDWTRRPGS
                     VGKPLLGVAHILDENGDDLPAGQAGQVYFDIGEVAFEYHRDPEKTAASRSRHGWVTVG
                     DVGYLDDDGYLYLTDRRHHVIISGGVNIYPQEAENLLVTHPKVLDAAVFGIPDDEMGQ
                     SVKGVVQTVDPADATEEFADELLGWLRERLAHYKCPRSISFEAQLPRTDTGKLYKQEL
                     VAKYSAARQ"
     gene            8926..9906
                     /locus_tag="VF874_08110"
     CDS             8926..9906
                     /locus_tag="VF874_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9176046.1"
                     /translation="MSGGPYFDELHVGQVFDSAPSMTLTQGVAAAHQAILGDRLRLPL
                     DAHLSYAVTEATAALAHPGLVCDVAIGQSTLVTHHVKANLFYRGLVFHRFPVIGDSLF
                     TRTEVVGLRQNSPKPGRAPTGMAALRMTTIDQLGQLVLDFYRCAMLPLSPDADPNREP
                     PADDLSAIGGEPVTLTKAVEEWDADAFRARVPGPHFDPGLTGSVLHSSADVVSSAPEL
                     ARLTLNVAAIHHDSRASGRRLVYGGHTIGLALAQASRVLPNLVTVLGWESCDHTGPVH
                     EGDTLVSELHVEAAEPLPHGGGILRLRSLVSAVGEDERPVLDWRFTVLQF"
     gene            complement(9887..10786)
                     /locus_tag="VF874_08115"
     CDS             complement(9887..10786)
                     /locus_tag="VF874_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9176047.1"
                     /translation="MPRDNGIHPDLRMLARFLPPTSVTPRTLWVIRALESLPIGGRKG
                     VEVLTLTSGRRVRLHTPPHQNGPAPAMLWIHGGGYLFGNLRYDDELCQRFSRTLGIVV
                     AAPEYRVAPEHAYPAALEDCYAALEWLSSLPSVEPARIAIAGESAGGGLAAALALLAR
                     DRGEFTPALQLLSYPMLDDRTVAKPGAASSYRLWNERSNRFGWKSYLGGADPDIAVPA
                     RRTDVAGLPPAWIGVGTADLFCDEDIAYAERLKDAGLPCHLEVVPGGFHGFDRIAART
                     SVARAYFDSQCQALRGALRTAAP"
     gene            complement(10776..12407)
                     /locus_tag="VF874_08120"
     CDS             complement(10776..12407)
                     /locus_tag="VF874_08120"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9176048.1"
                     /translation="MDADVIFSGDVVTVDDAHPTAEAVAVTNGRIAVVGARDQVIAQH
                     RGAGTRVVDLDGNTLLPAFIDPHSHYINSLMVANQVNVYAPPAGPGTDVAAIITALRN
                     FRDTKRIPPGEVIMGYGYDDTLMPGGRTLHAEDLDVDFPDNPVIVQHVSLHGGVLNSA
                     ALRKWGITADTPTPPGGVIVRKPGGHEPDGLLMETAFLPVFASLPKPTPDQEIAWSKA
                     GQALYAAAGITTAQEGLSHQADIDLLYRAADGGATLIDIVAYPFIFDVDAVLQKYPPS
                     TFGKYRNRVKLGGVKITLDGSPQGRTAYFTTPYLADGPEGQHNWRGEQPFPQDTVTGW
                     FKHVYDLGLQLLIHANGDAAIDMLLGAHEAASAGDKPGADRRTTAIHSQFVRRDQLQR
                     YLAQRVIPSFFTSHAYYFADAHIRLRGREQADFLSPMRAAIELGLRPTNHTDFNVTPI
                     DQLFLMWTAVNRVSRGGEVIGEDQRATPLEALKAVTINAAYQYFEEDSKGSIEVGKLA
                     DLVILDANPLTVDPMTIKDIKVVETLKEGQTIYAA"
     gene            12474..>13268
                     /locus_tag="VF874_08125"
     CDS             12474..>13268
                     /locus_tag="VF874_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9176049.1"
                     /translation="MDSRHPGQRKPNLQSRAMWFVRARPADYLLAMSEISGSLPLVGK
                     YFQPFGGITAMGVWGYRHAPDFVASAIRSRNRAGAEQRRQHRMMTHDVASDALKDFVP
                     PERQKVEWPAPDRLAPVLKGVGQRRRYLHRGAVRYGDAPSQVLDVWRRPDLQGPAPVL
                     VFVPGGAWVVGSRILQGYTLLSHLAEQGWVCLSIDYRVSPHHRWPRHVTDVKAAVAWA
                     RANVDRFGGDREFVAIAGCSAGGHLAALTGLTHDDTDFHVDLADDAD"
BASE COUNT         2147 a         4421 c         4527 g         2173 t
ORIGIN      
        1 ggcgcgggag ctgtcgtgcc ggcgtcagca cagcgactgg gcgcccatgg ttggtgatcg
       61 tcaccgacac gccggtccgc tcgacctctg ccaagagcgc gttgagcttg gccttagcct
      121 ccgtgctggt gacggccttc atacgttcca cgttaacacc cacggactat tcagactagt
      181 tacgctagtc aagcggtaga gatgtgcctc tcgcgccgca ttagtgcgca cggaggaggc
      241 ccgcgcgggc ctggtcgatc tggtcgagcg cgtaggtcgc catggtggcg ttggtcatgt
      301 gctcgctggc gcgggcgaat gattcgtaga cctcgtcgcc gagtaattcg cgcaggtggg
      361 tgatcgcgcg gttgatctcg gggaaggcag tccgagaaag cggagtcgcg gcgaacccac
      421 agatggtggc ggccggttcg tggtgtccga gtcgatcgaa tatggcggcc aggactgcca
      481 gtggggtcgg caggatggag aagctgccgg agtcgtagag gttgcgaatg gccacgctca
      541 ggaagtgaaa cgcgttggtg gggtcggcgt gggtggccgc cagcctggac aggccgaccg
      601 tcaggatcga ctcgaccctt cggttaccgc tctcttgggc gatcctcagg cctcggcgaa
      661 ggacgtcata ggcaagttcg gggtcggcgt cgccgcgggc aaatccgtag gcgaagagcg
      721 cccagctgac tacatgggga ttatcggtgg cgtcggtagc gatcaggcct tcggagacag
      781 ccatcgcttc atcaccggcg ccggcgattg tcagtgccag agccaggctc gatcgggcaa
      841 cggtgcaagc gccgccagga cttcgcgcga cgatgttgcg gtatacctcg acgcatcgtt
      901 cgagttgacc attggcgagg tatgcaccgg cgagtatgcc ttcgaactcg cggcggacgt
      961 cgtcgaatcg cccactttca atggccgcgt ggccggcgtc gaggtagccg acggcgtcgt
     1021 cgatccgccc ggtcacgtgg cactggcgat cgcggccgcg gtgtcgagaa gcctcgcgct
     1081 gtcgcggact gtcccacagc gcaagcacat cggcctcgcg ctctgcgaag tggcgcgcat
     1141 gcgcggtgcg cgcagccttg gcctcaccgg tcgccgtgag ttgttcttcg ccgaattgac
     1201 ggatggtctc caacatcgag aaccgcctct gttcggctga tcggtcggcg acaagtagtg
     1261 acttgcgcac cagggcatcg aggagatcga gtgtttcgaa ttcgtcgtcg gaacctgcca
     1321 ccgcatgtgc tgccggtagg tcgaacccac cggcgaacac cgaacacctt gacagcaagg
     1381 acttttcggc gtcgccgagc aggtcggagg accactgcac agcgtggcgc aatgtctggt
     1441 ggcgctaaag gccacggcgc gaccccacga ggagccgaaa ccgatcatcg aggcgatgct
     1501 gcagttcctt gatcgtcatc gactgcatgc gcgatgcggc caactcgatt gccagcggca
     1561 tcccgtcgag gcgccggcag atctcgacca cagcgcccgc gtcatcatcg agcgaaatcc
     1621 ccggcgccac agccgccgcc cgttcggcga acagcatcgc cgcacccgaa ccgatgtcca
     1681 gtgacggcac cgaccagagc tgttcgtcgg ccacccgcaa cccttctcgg ctggtggcga
     1741 ggacttgcac ggccgccgac tgcgagagga tcgcctcggt caactcagcg gccgcctcga
     1801 ggacgtgttc gcagttgtcg aacacgagca gccgcgtgcg gccctccatc gctgccgcga
     1861 cgctgtcggc cagactcatg ttcggctgct gggtgatccc cagcacggcc gccaccgcct
     1921 cgggcaccgc ggcagggtca gcgaccgtgg ccaactcgat cacccacacg ccgtcgggga
     1981 agttgtcggc caaccggtcg gcaacctcca acgccaaccg cgtcttaccc accccgccgg
     2041 cgccggtcaa cgtcatcaac cgatgagact tgagcgcctc ctgcacctcg acgagttcat
     2101 tcacgcggcc gacgaagctc gtggcgggcg ccgcagattc ccgggcaccg cgtcgagtgc
     2161 cttcaggggc ggaaactcgc tacgcaggcc ttcaccttga acctgatata tgtcgaccgg
     2221 tttggcgata tcccgtaacc gccgtggccc cagactaatt aggtcgatac tagtgagcag
     2281 gcccgcggtg gcgccgtcga gcaggatctg cccgccatgg cccgccgcca tcacgcgcgc
     2341 cgcgcggttg agcacggccg aagtcgtccc tgccacgtaa ctcggcctcg ccggtggaaa
     2401 tacccatccg cacaggcagt tcgagtttca gttgcgccat caccgcgggg tcaacggcag
     2461 cccgcggtga ggcaaacact gcgcacactc cgtcgccggt gtgcttgaac aaccagccct
     2521 cgtgcgcctc aatagcctcg cgcaacaccc gatcgtgggt ttcgagtgcg ggcctcatgg
     2581 catccgcatc ggcctcccac cgcctcgtcg acccctcgat gtcggtgaac aggaaagtga
     2641 caaccccaga cggcgcagtt ctagccttct gaacgcgttt catcggggcg cccgctcgct
     2701 cagtggagta cctcgtcccc tcgtttacct ggcgatgcca tggtaggtcg tgaacagggg
     2761 tggcacggag gaattcgacg ggctcaaccg ttgcctcggc tgaacgccga cgggtcggcg
     2821 gtcgcctcga ttttcgcgac gaggccgttg cgcacggtca ggacgagaac ggcgaacagc
     2881 tgcctatgcg cgaaacccag caggaccggc tgcccgacgg gggcgctgac gagcgtggcg
     2941 ccgtgcccga ggtagcgcat caagttggtg gccaccgcgt ccgggccgtg gttgacctga
     3001 gggggcggag ccggatcgcc cagaagcgtc gccgcacccc agacagtcgg gtcgagtacc
     3061 gcggtcagcg ccgccaaatc gcctgtggcg caagcagaga tgaatttctc gatgacggcg
     3121 cgatgttcgt cgcgggtcac gtcagtagtc gccgggcccg acgcctcgat cttggcccgg
     3181 gcgcgacggg ccagctgccg gcatgttccg accggccgcc caaccgtttc ggcgatctca
     3241 acgaagggca ccttgaacac atcgtgcagc acgaacgtga cccgctcgcc cggactgagc
     3301 cgccgaagca cctgcagcag ggcatcgcca acctgatcat cgagcgtgac gcgatccgcc
     3361 gggtcgacga tcccatgcgc tagcgcttcc tccaatatcg ttgtgtcggt ggctgattca
     3421 tatctgtgcc gcgccgagcg aaccaggtcg aggcacaacc ggccggcgac gaccgtgagc
     3481 cacccgcgga cgtcgtcgat gccgtcgaca cccgacaggc gggcgaaggc ctcttgagcg
     3541 acgtcttccg cgtctccgat atcgccaagc atctgatagg tcaggttgac gacatacggg
     3601 tgatggcgcc gccacgcctc ttgcagctgc gcgtccgtca tgcccatacg acgaagcaaa
     3661 ccaccaaaaa gttacgcgcg cgggccgtaa ctttccgggc cgtcgctccg tccttgtttc
     3721 agcgctcgag aaaggagttc gatcatgcca atcgttgtca ccggagcaac aggcaacgtc
     3781 gggcgaccgc tggtcgcgga gctggtcgcc gcaggtgtcg aggtgcgggc ggtgagccgc
     3841 cgtccggaca ctgccgcgtt tcccggcgag gtgaaggtgg tcgatgccgc ggcagcagcg
     3901 attcccggcg cttccgcggt gttcctcaac tcgcgtgcgc tgggcgacga catggagtcg
     3961 gtggtccggc tggcgcaaag gcacgcggtc acgaagctgg tggcattgtc ggcgatcaac
     4021 gccgacgacg acttctcgcg gcagccgtcg cggtaccgcg gcgaccgcaa caaggagatc
     4081 gaacagctcg ccgtcgcttc ggggttgccg tgggtcagtc tgcggccgtc ggtgttcgcc
     4141 accaacttcg cgggcatgtg ggcgggtcag atccgggccg gcgacgtggt caacggaccg
     4201 tatgcggccg catccagcgc accgatcgtc gagcgtgaca tcgctgcggt ggctgcccgc
     4261 gctttgcttt ccgacgagct tgtgggacag cggattccgc tgaccgggcc gcaggcgctg
     4321 accaacaccg agatggtcga catcatcggg tcggtcctac accggccgtt gcgctacagc
     4381 gaaattcccg ccgagctggt gcggcagcgg ttcattgcgt tgggttttgg caccgagttc
     4441 gcggacgctt acatcgcgat gctcgcgacc acggtcgatc ggccggcgct ggtcacccac
     4501 gatctccaaa ggattctcgg tcgacccgcc gagacattcg ggcagtgggt ggccgaccac
     4561 gtcagcctgt tcacgaatta gcaagggaga gagaagatgt cgaatccgca gccaccgcgc
     4621 tatctcaagc cgatgaacaa gatcatgatg gcgatccaga agctcggcat cccgactggg
     4681 cccgcgatgg tgctgaccgt gcctgggcgg cgatcgggac agccgcgtag caccccgatg
     4741 acgccgttca cgcatgacgg caagctctac accgtcgccg gatatcccgg cgccgactgg
     4801 ccgcggaacg cacgggccgc cggcgaaggg acgctcagcc gaggccgaaa gtcgaggcgc
     4861 gtcaagatcg tcgagttgtc tgccgacgag gcgggtccgg tgctgcggga gtttcccggc
     4921 caagtgcccg tcggcgtcgg cttcgccaaa cgctcagggc tggtggtcga cggcacgccc
     4981 gacgaattcg aggcgctggc cgggcagctt gcggtgttcc ggttcgaccc gcttacgttc
     5041 taagcgaagt gtcggcgcag ccgtcggtgg cgatgccgga agaggatcgg gactgctgcg
     5101 ccgaacacgg cctcgaacgc cctcgtgcgg ccttgccagt gcagccggtc gatgacgcgg
     5161 cagccgccgt cctgcggctc gacgatgcgt tcgtgcgacc actgcgattg cgtcaacatc
     5221 gtcgaacgct ccaggaagcg gcggcccggg tcgacctcga cgagggcaag gtcgtcgtag
     5281 tccaccggga cgagcccgag cagcaacagc cagcaccgcc cgattcgctg ccgcagcggg
     5341 atctggtcga tcgtggcgcc gcgcagatgc cggggcatcg tcatccgcat cagcggcagc
     5401 agctcatcgt tgatgccggc cggggtcacc acgcgctgcc agactgcttc cggactcgcc
     5461 gccaggtcgg acgcgatctc gaacgaacgc actgggctac tcggcggacg gcagttcgcg
     5521 cgggttgccg cggccgcgca accgagcggg gccgtcccac tgcccgatct cgggctgacg
     5581 ctcgcgtgcg ctcaacccgt aagcctcgtc ggcggctgcc cacgccaacg actcgctctc
     5641 ctcgggccgg gacgactcga cccggcgcgc gtcgtcgcta gccgccggac cccgctgctg
     5701 tgaatgctct ggcgtctcct cgtcgaagac cgtcaccttg tgggtgcgct tgaacgagaa
     5761 cgtgatctcc tcgacctctt cgaagacctg ccgcgcggtg cgcagcgggt gggtcgtggt
     5821 gtcgatcgcg cgggcgacga tcgggggcac gagcgggcgg atccagcggg ccgcggtctt
     5881 ggtgacgttc gggatcgtcg gacgcgcggg cgtgcgttcg tcctccacgc gcgcgggcag
     5941 gttatctgcg gcgggcagcg cgcctcggct cgcggcttcc gcctcggcgg cgatcggcag
     6001 gtacatgtct tcctccccgg gttcggtggg ggtactcccc cgcatgcggg aggtgccccc
     6061 acccacttgc gggggagggc gctgcagcgt ggcctgcacc ggagcatcgt cgagcgcctc
     6121 gacgacgcga cgtcggtgca actctcggtc gatttcgtgc tgtgcctcga ccgcgagccg
     6181 ggcactggcg agctgctgct cggcccagat ggtctcgatg gcggcgcgca cctcggcgcg
     6241 cttgacggcg atcgcggtgt ccgcgttgag ttcggcgcga tcgcgctggg cgcgcgcggc
     6301 cgcgccgcgg tcgtggctgg tctcgccccg cagcagcttc aggatcaacg gcagcaggct
     6361 cagcagcgcg aagaacgcga ccgtgaccgc ccacaccacc agcgcgcccg cgccggccag
     6421 cgtgaccgcg tgcatcgcga cccagcgcgc gccgaggcca cggtccgcag ccgcgaccgc
     6481 tgcctcacgg gactgcgtca caccctgttc ggcgccggcg atctgcgcgt tcagtgcggg
     6541 cgtctgctgg tcccgcacgg cgacggcgtt gtccagttcg cgctgggtgt cccggagaat
     6601 ctggttggcc gatcgggttt cgggcccgga gccgggaact ccggtgatgc gggtctgcgg
     6661 gcagccaggt gtcgggttga actcgcagcg ggcgacgacg agcgcctggt cgcggtgggc
     6721 gcgggcctgc tccaccgcgg tgtcgagcgc ggtgcgggtg tcgcgcatcc ggccgaggtt
     6781 cgcctcggcc tgcgcgacgg cgggtgtggc gctggccgaa cgggcggcgt cttcgtcgat
     6841 gcggcggtcg atcgacccgg agaacagcac caccgcggcg agttcgccga cgacgaggcc
     6901 gacgagggcg gcgactgcac cccgccctgc gacggcccac ccgcgcgtcg ggccgctggc
     6961 tgcggcgcgg ctgaccgcgc cgacgagcag cccgaacagc agcgtcaacg gcaggatcgc
     7021 ccacaccggc cactgcgtgg actcggcgac ggcgaccgtg gcgaccagcc aggtcagcgc
     7081 ggcgccgacc agcaccacca cgccggcgat cgcggcggtg gagcgctcgt gacgctcgcc
     7141 gagttcgcgc cagtgcccgc cgccgagcca ggtcagcacc gcctcggcgc ggcccgagtt
     7201 cggttcagaa ttcttctgcg cgcacatggg gacgacaaac acctccagtt ccccagggtg
     7261 acagggcgat acccgacaca ccgaatccaa caggcggact gtggcgcggt tcacacggat
     7321 ttttcgcacg cccggccgcg acctgcatcg acgcccgtca actacagtgc tgcgcatgca
     7381 gatccgcgag cacgtctcca ccaagccggc cgtcatcttg caccccacgg gcaccgtggt
     7441 gacgttcgcg gagctggagg cgcgcgccaa ccggctgtcg caccactttc gcagggccgg
     7501 gctgcgcgag ggtgacaccg tcgcggcggt cttggagaac aacgagcacg tgcacgcggt
     7561 gatgtgggcg gcgcgccgct gcggcctgta ctacgcgctg atcaacacgc acctgaccgc
     7621 ggcggaggcg gcctacatcg tcgagaacag cggcgcgaag gcggtcatcg ggtcacgcgc
     7681 cacgcgaccc atctgcgagg gcctcgcctc gctgactgag ctgcccgagc tgttgctcat
     7741 cgccgacgga gccgctgggt cggcgacatc cgcctcccgc gatctcgatg gctggcagcg
     7801 ctatccggag tgtgtcgccg acgagccgga taccccgatc gacgacgagc gcgagggcga
     7861 ccttctgcag tactcgtcgg gaaccacggg gcggcccaag ggaattcgtc gaccactgcc
     7921 gcacgtcacc ccggcggaag cgcccaattt gctgagtgtc ctgctgatgg cgataggcat
     7981 gacgcccgaa gcggtgtatc tgagccccgc gccgctgtat cacaccgcgc cgtgcgtgtg
     8041 gtcgacgtcc gcgctggcga tgggtgcgac cgtggtcgtg atggagaagt tcgatccgga
     8101 gcagacgctg gaggcgattc agcgctaccg cgtcacacac ggccagttcg tcccggcgat
     8161 gttcgttcgc atgctgaaac tgcctgaggc ggtgcggaat tcgtacgacg tgtcaagctt
     8221 gaagcgcgtg gtgcacgcgg ccgcgccgtg cccggtggag atcaagcagc agatgatcga
     8281 ctggtggggg ccgatcgtcg acgagtacta ctcgtcctcg gagggggccg gcatcacgtt
     8341 catcacggcc gacgactgga cgcgacgccc cgggtcggta ggcaagccgc tgctcggggt
     8401 cgcgcacatc ctcgacgaga acggcgatga cctgcccgcg ggacaagccg ggcaggtcta
     8461 cttcgacatc ggcgaggtcg cgttcgagta ccaccgcgac ccggagaaga ccgcggcgtc
     8521 gcggagcagg cacggctggg tgaccgtcgg cgacgtcggc tatctcgacg acgacgggta
     8581 cctgtatctc accgaccgcc gccaccacgt gatcatctcc ggcggggtga acatttaccc
     8641 gcaggaggcc gagaacctgc tcgtgacgca cccgaaggtg ctcgacgcgg cggtgttcgg
     8701 catccccgac gacgagatgg gccagtcggt caagggcgtc gtgcagaccg tcgaccccgc
     8761 cgacgcgacc gaggagttcg ccgacgagct gctgggctgg ctgcgagaac gcttggcgca
     8821 ctacaaatgt ccacgctcga tctcgttcga ggctcagttg ccgcgcaccg acactggcaa
     8881 gctgtacaag caggaactgg tcgcgaaata ctcggcggca cgccaatgag cggcggaccg
     8941 tatttcgacg aactgcatgt gggccaggtg ttcgactccg cgccgtcgat gacgctgacc
     9001 caaggtgtcg ccgccgcgca ccaggccatc ctgggtgacc ggctgcggtt gccgctcgac
     9061 gctcatcttt cgtacgcggt tacggaagcg acagcggcgc tggcacatcc tggtctggta
     9121 tgcgatgtcg cgatcgggca gtcgacgctg gtcacccatc acgtcaaggc aaacctgttc
     9181 taccgcgggc tggtgttcca ccgcttcccg gtgatcggcg acagtctgtt cacccgtacg
     9241 gaagtggttg ggctgaggca gaattcgccg aaaccgggcc gcgcacccac cggtatggcc
     9301 gcattgcgga tgacgacgat cgaccaactc ggccagttgg tcctcgactt ctaccgctgc
     9361 gcgatgctgc ctctgagccc ggacgccgac ccgaaccggg agccccccgc cgacgacctg
     9421 tcggcgatcg gcggggaacc ggtgacgctg acgaaagccg tcgaggaatg ggacgccgac
     9481 gcgttccggg cacgggttcc agggccgcat ttcgaccccg ggttgactgg ctcggtgctg
     9541 cacagcagcg ccgacgtggt ctccagcgcg cccgagctgg cccggctgac cttgaatgtc
     9601 gctgcgatac accatgattc acgtgccagc ggccgccggc tggtgtacgg gggccacacc
     9661 atcgggttgg cgctggccca ggccagccgg gtgctgccca acctggtcac cgtgctgggg
     9721 tgggagtcct gcgaccacac cggaccggtg catgagggag acacactggt gagcgaactg
     9781 cacgtcgagg cggccgaacc gctacctcac gggggcggca tcctgcggct gcgctcgctc
     9841 gtcagcgcgg tcggcgagga cgagcgcccg gtgctcgact ggcggtttac ggtgctgcag
     9901 ttctgagcgc cccgcgcagt gcctggcact ggctgtcgaa gtacgcacgc gcgacggacg
     9961 tgcgggcagc gatgcggtca aagccgtgga atccgcccgg caccacctcg agatggcatg
    10021 gcaggcccgc atctttcaga cgttccgcat aggcgatgtc ttcgtcgcag aacaggtcgg
    10081 cggttccgac gccgatccat gccggcggca gcccggccac gtcggtacgg cgcgccggga
    10141 cagcgatgtc ggggtcggcg ccgccgagat acgacttcca cccgaaccgg ttactgcgct
    10201 cattccacaa tcgataactc gacgcggcac cgggtttcgc gacggttcgg tcatccagca
    10261 tcggataact caaaagctgc agggcagggg tgaactcacc ccggtcgcgc gccagaagtg
    10321 ccagcgcggc ggcaaggcca ccaccggcac tttcgccggc gatcgcgatc cgcgccggct
    10381 cgacagaagg cagcgatgac agccattcca gtgccgcata gcagtcttcg agtgccgccg
    10441 gatacgcgtg ctccggcgcg acgcgatatt ccggtgctgc gacgacgatt ccgagcgtcc
    10501 ggctgaaccg ttgacataac tcgtcgtcgt atcgcagatt tccgaacagg tatccgccac
    10561 cgtgaatcca caacatcgcg ggggcgggac cgttctggtg cggcggcgtg tgcagcctta
    10621 cccgccggcc ggacgtcagc gtcagcacct cgacgccctt cctgccgccg atcggcagcg
    10681 actccagcgc acggatcacc cacaacgttc ggggcgtcac cgacgttggc ggtagaaacc
    10741 gtgccagcat ccgaaggtct ggatgaatgc cgttgtcacg cggcatatat cgtctgtccc
    10801 tctttgaggg tctcgaccac cttgatgtcc ttgatcgtca tcggatcgac ggtgagtggg
    10861 ttggcgtcga ggatcaccag atcggcgagc ttgcccacct cgatggagcc cttgctgtct
    10921 tcctcgaagt actggtacgc ggcgttgatc gtgacggcct tgagcgcctc cagcggcgtg
    10981 gcccgctggt cctcgccgat cacctcgcca ccgcgcgaca ctcggttcac cgccgtccac
    11041 atcagaaaca gctgatcgat cggtgtgacg ttgaagtcgg tgtggttggt cggccgaagg
    11101 ccgagctcga tggccgcccg catcgggctc aggaagtcgg cctgctcgcg gccgcgcagc
    11161 cggatgtgcg cgtcggcgaa gtagtacgca tgtgaggtga agaacgatgg gatcacccgc
    11221 tgagcgagat agcgctgcaa ctgatctcgg cgcacgaact gtgaatggat cgcggtggtc
    11281 cggcggtcgg cgccaggctt gtcccccgcc gatgcagctt cgtgcgcgcc cagcagcatg
    11341 tcgatcgccg cgtcgccgtt ggcgtggatg agcagttgaa ggccgaggtc gtagacgtgc
    11401 ttgaaccatc cggtcaccgt gtcttgcggg aatggctgct cgccgcgcca attgtgttgt
    11461 ccctcaggac catcggccaa gtacggcgtc gtgaaatatg cggtgcggcc ctgcggcgac
    11521 ccgtcgagcg tgatcttcac gccgcccagc ttcacccggt tgcggtactt gccgaacgtc
    11581 gacggcggat acttttgcag caccgcgtcc acatcgaaga tgaacggata ggcgacgatg
    11641 tcgatcagcg tggcgccgcc gtcggcagcg cggtacagca ggtcgatgtc ggcctggtgg
    11701 gacaggcctt cctgggcggt ggtgatgccg gccgccgcgt acagcgcctg ccccgccttg
    11761 ctccacgcga tctcctggtc cggcgtcggc ttgggcagcg acgcgaacac cggcaggaac
    11821 gcggtctcca tcaacagccc gtcgggctcg tggccgccgg gcttgcgcac gatcaccccg
    11881 ccgggcggcg tcggggtgtc cgccgtgatg ccccacttgc gcaaggcggc ggagttcagc
    11941 accccgccat gcagcgaaac atgttgcacg ataacgggat tgtccggaaa gtcgacgtcg
    12001 aggtcctcgg catgcagggt acgtcccccg ggcatcaacg tatcgtcgta gccgtagccc
    12061 atgatcactt cgccaggcgg aattcgcttg gtgtcacgaa agtttcgcag tgcggtgata
    12121 atcgcggcca cgtcggtgcc cgggccggcc ggcggggcgt agacgttgac ctggttggcc
    12181 accatcagcg agttgatgta gtggctgtgc gggtcgatga acgcgggcag cagcgtgttg
    12241 ccgtccaagt cgacgacgcg cgtcccggcg ccgcgatgtt gggctatcac ctggtcgcgg
    12301 gcccctacca cggcgatccg gccgttggtg acagccacgg cctcggccgt ggggtgcgcg
    12361 tcgtcaacag tgacgacgtc gcccgagaag atcacgtcag cgtccacgat tggacgctaa
    12421 cctgcgcccc ggatttccgt ttccaaataa cggcacagga gtacggtgtc cgaatggatt
    12481 cccgacatcc ggggcagcgc aagccgaatt tgcagtcccg ggcaatgtgg ttcgtccgtg
    12541 cgcgtcccgc cgactatctc ctggccatga gcgagatctc tggatcgttg ccgctggtcg
    12601 gcaagtattt ccagccgttc ggtgggatca ccgcgatggg cgtgtgggga taccggcacg
    12661 caccggactt cgtcgcgtcg gcgatccgca gccgcaaccg cgccggcgcc gagcagcgcc
    12721 gccagcaccg gatgatgacc cacgacgtcg cgtccgacgc gctgaaagac ttcgtgccac
    12781 ccgagcgtca gaaagtcgag tggccggctc cggatcggct ggcgccggtg ctgaagggtg
    12841 tggggcagcg gcgccgctac ctgcaccgcg gcgccgtccg ctacggcgac gcgccgagcc
    12901 aggtgctcga cgtgtggcga cggccggacc tgcaggggcc cgcgcccgtg ctggtgttcg
    12961 tgccgggcgg cgcctgggtg gtgggcagcc ggatcctgca gggctacacg ctgctgagcc
    13021 acctcgccga gcagggctgg gtgtgcctgt cgatcgacta ccgggtgtcg ccgcaccacc
    13081 gctggccgcg gcacgtcacc gacgtcaagg cggcggtcgc gtgggcgcgc gctaacgtcg
    13141 accggttcgg cggcgaccgc gaattcgttg cgatcgcagg ctgttcggcc ggcggtcacc
    13201 tcgcggcgct gaccggcctg acgcacgacg acaccgactt ccacgtcgat ctcgccgacg
    13261 acgccgac
//
LOCUS       DASWBF010000228         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_221735,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000228 DASWBF010000000
VERSION     DASWBF010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221735"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..882)
                     /locus_tag="VF874_08130"
     CDS             complement(73..882)
                     /locus_tag="VF874_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9176050.1"
                     /translation="MSTDPALAAKHRAMWASGDYPAVVTDLVAPLGPILIEATGIGPG
                     DRVLDVAAGTGSVAIPAAQTGATVVASDLTPELLTRGQAIAAERGVALEWREANAEDL
                     PFGDNEFDAVVSSIGVMFAPHHQLAADELVRVLQPGGRIGVISWTPEGFIGQMFATMK
                     PYVPAPPPGVQPPPLWGSEDRVRALLGERVEEFSTHRGTLTVDHFATGAAFRDYFKAN
                     YGPTITAYRSISDDAERVAALDAELAELGDRHLAGSTTMEWEHLLVTARKR"
     gene            927..1127
                     /locus_tag="VF874_08135"
     CDS             927..1127
                     /locus_tag="VF874_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176051.1"
                     /translation="MTTPDDPVAVLHRWACAGALWRVIGRHDAGVTVRLYRCDGGEEV
                     DRISSTDPRLLHYLAGRTSSEE"
     gene            1288..2091
                     /locus_tag="VF874_08140"
     CDS             1288..2091
                     /locus_tag="VF874_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007240945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX9176052.1"
                     /translation="MKATVTPEAENAITDVAQRHGLSREAVLAMLYAIHVGGGTMAQF
                     SISELGGSGQWMQGGMTMVGGNMFDNALRARVDALCTELAQLLATTSVFPPSANWWPA
                     NLGVPSAAGGQNTARYAIFPSTRRLAIQINGVTKVFDTGEHRIGGVQQQQQGGGYGSV
                     TFTSQFGTFDVSSLPEVGTQRAAETPGAARAPQYQSQYQSDPAQAQYQSDPAPQFAPQ
                     TQTPEPASGASGDPAAIVAAIESLAGLHQRGILSDEEFAAKKAELLGQL"
     gene            2372..2689
                     /locus_tag="VF874_08145"
     CDS             2372..2689
                     /locus_tag="VF874_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176053.1"
                     /translation="MAVTTADPRSGDDTFDLIDNAVAALADRRGVWLGHDLHAIALIA
                     SLVEQAERWLPQLVHDARANGYDWTEIARAVGTNPNEARLRFDPNPQSPTGSVSTSKE
                     QVP"
     gene            complement(2827..3219)
                     /locus_tag="VF874_08150"
     CDS             complement(2827..3219)
                     /locus_tag="VF874_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176054.1"
                     /translation="MHRNLLQRHKDLASLIDQAAGGHRPDQHLRPQDRVTRESLLAEL
                     AAAKHRNRELQQQVRVLERRLGAQGPSIGAALIDQHPVVIELRKRLAEVELAAMAKDR
                     TITSLHDDVEVLRETNRSLVREYGLTRS"
     gene            3317..3451
                     /locus_tag="VF874_08155"
                     /pseudo
     CDS             3317..3451
                     /locus_tag="VF874_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_052642687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          604 a         1242 c         1240 g          616 t
ORIGIN      
        1 agcggcgggc tagcgggggc ttttgcgttc ccccgcaagc gggaggtgcc ccagctcgcg
       61 gggagaagaa gatcagcgct ttcgcgccgt gacgagcagg tgctcccatt ccatggtcgt
      121 cgaccccgcc aagtgccggt cacccagttc ggccagttcc gcgtccagcg cggcgacgcg
      181 ctcggcgtca tccgagatgc tgcggtaggc tgtgatcgtc ggcccgtaat tggccttgaa
      241 gtagtcgcgg aacgcggcgc cggtcgcgaa atggtcgacg gttagcgtgc cgcgatgggt
      301 gctgaattcc tccacgcgtt ccccgagcag cgcccgcaca cggtcctcgc tgccccacaa
      361 aggcggcggc tgcacacccg gcggtggcgc gggaacgtac ggcttcatcg tcgcgaacat
      421 ctgcccgatg aagccttccg gcgtccagct gatcactccg atccgaccgc ccggctggag
      481 cacgcggacg agttcgtcgg cggccagctg atggtgcggc gcgaacatca caccgatgct
      541 tgacaccacc gcgtcgaact cgttgtcccc gaacggcaaa tcctcggcgt tggcctcccg
      601 ccactccaat gcgacgccgc gctcggcggc gatcgcctgc ccccgggtca gcagctcggg
      661 cgtcaggtca ctggcgacga cggtcgctcc ggtctgcgcg gccgggatcg cgacgctgcc
      721 cgttcctgcg gcgacatcga gaacccggtc gccgggcccg atacccgtgg cctcgatcag
      781 tatcgggccg agcggggcca ccaagtcggt gacgacggcc ggatagtcac cggatgccca
      841 catcgcccgg tgcttcgccg ccagcgcagg gtcggtgctc atgttacctt ggtccctcac
      901 aggccaggcg tgggaggcag aacggcgtga cgacgcccga cgacccggtc gccgtgctgc
      961 accgctgggc ttgcgccggg gcgctgtggc gggtgatcgg ccgccacgac gccggggtga
     1021 cggttcggct gtatcggtgc gacggcggcg aggaggtcga ccgcatcagc tcgacggacc
     1081 cacggttgct gcactacctg gcgggccgca ccagcagcga ggagtagccc gtcacatcaa
     1141 cccggcggtg cgcgcgaggt tgagtatccc gctgcggctg agagacgatg tcctccagcc
     1201 ggctcagcgg caggtccatg tgccactcgg gcagcagccc gtacagcaac acgcaactgg
     1261 gtatcgacga cgaaagattc acaatttatg aaggcaacgg ttacccccga agccgaaaac
     1321 gccatcaccg acgtcgccca gcgccacggg ctctcccgcg aggcggtgct ggccatgctg
     1381 tatgccatcc acgtcggtgg cggcaccatg gctcagttct cgatctccga gctcggcggg
     1441 tccgggcaat ggatgcaggg cgggatgacg atggtcggcg gcaacatgtt cgacaacgcg
     1501 ctcagggcgc gcgtcgatgc gctgtgcacc gagttggccc aactgcttgc caccacctcg
     1561 gtgtttcccc cctcggccaa ctggtggccg gctaacctcg gcgttccgag cgcggcgggc
     1621 ggccagaaca ccgcgcgtta cgccatcttt ccgagtactc ggcggctggc gatccagatc
     1681 aacggggtca caaaggtttt tgacaccggc gaacaccgta tcggtggcgt gcagcaacag
     1741 cagcagggcg gcggatacgg ctccgtgacc tttaccagcc agttcggcac cttcgacgta
     1801 tcgagcctgc ctgaggtagg tacgcagcgg gcggccgaaa ctcctggcgc ggcacgggcc
     1861 ccgcagtacc agtcgcagta ccagtccgac ccggcacagg cgcagtacca gtccgacccg
     1921 gcaccgcaat tcgcacctca gacgcagaca cccgagcctg cttccggcgc gtccggggat
     1981 cccgcggcca tcgtcgcggc catcgagtcg ttggccggac tgcatcagcg tggcatcctc
     2041 tccgacgagg agttcgccgc gaagaaggcc gaactgctcg gccagctctg aggcgctacc
     2101 gcatccgcgc agaccgtaag tgggcgataa tccgggggtt gacaactttc tctacagggg
     2161 tctccttatc agcggagcga atccggtccg tgagggagcg cggaagttgc gccacgtccg
     2221 gcgggcagca cctcgacgac caggtcgatc aggcgctgtg cctcaccctc gttaccaata
     2281 ccacgctcac gaatctgttc gacccaggta ccaagaggcc atcacgcagg agttaccgct
     2341 accttcggcc ctatacagcg accgtgagga catggccgtg accaccgcgg atccgcggag
     2401 tggcgatgac acctttgatc tcattgacaa cgccgtcgcc gcactcgctg accgccgtgg
     2461 tgtctggctt ggccacgacc tgcacgccat cgcgctgatc gcgagcctcg ttgagcaagc
     2521 cgaaagatgg ttgccccagc tggtccacga cgcccgggcc aacgggtacg actggaccga
     2581 gatcgcccga gcggtcggca ccaaccccaa cgaggcccgc ctacgattcg acccgaatcc
     2641 ccaatcgccg acaggctcgg tttcgacgtc gaaggagcag gtgccgtaga agttgatctg
     2701 gtcgtgctgg gcggggtcag gtgcgccgcg gcgcgtcgag cgcatcgctg aggtaggtcg
     2761 tagtccacag caccgcggcg tcgcccatgg tcgtcacacc ccccctcgtc gcgatgtcga
     2821 agcggatcag ctgcgtgtaa ggccgtattc acggacgagt gaacggttcg tctcacgtaa
     2881 gacctcaacg tcgtcatgca gcgaggtgat cgttcggtct ttcgccatcg cggcgagttc
     2941 gacctctgct aggcgcttcc gaagttcgat gacgacggga tgttggtcga tcagggcagc
     3001 gccaatcgat ggtccttgtg ctccgagccg gcgttccaag acccgtactt gctgctgcag
     3061 ttctcggttg cggtgcttcg ccgccgcgag ttcggctagc aggctctcgc gggtgacgcg
     3121 gtcttgcggg cggagatgct ggtcgggccg gtggccgccg gcggcctggt cgatcagcga
     3181 tgcgaggtct ttgtggcgtt gaaggaggtt ccggtgcaca ccggcgcggc tgatgacagc
     3241 ccggcggctg atagggcgct cctcgcgccg taggtccccg atcgctttgc tgcgccggcg
     3301 ccgcccccgc ctcggcgaca agtggcacct cgacgaggtg ttcgtgaaga tcaacggcat
     3361 caccggttac ctgtggcgtg gcatcgacca gcacggcaac gtcttggacg ttttggtgca
     3421 gtcgcggcga aacgccaaag cagccaagcg gttcttccgt aacgtgccgg gccggccagc
     3481 atccgcgccc aggcggtcac ggtgtggcgg cgcgcccacg gcggtgcgtg cggatcggcg
     3541 cgccacgctg cggccagccg gcaacccccg gggtctgggc cggcgcggcg tggtgtgccg
     3601 cgctcaccga cgccggcacg gcggcggctg cgggtagtaa cccatttttt aacgggagat
     3661 ttttccctta cgggagaccc ttcgggatgg ggtgacagcg ct
//
LOCUS       DASWBF010000229         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1005073,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000229 DASWBF010000000
VERSION     DASWBF010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005073"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VF874_08160"
     CDS             <1..1382
                     /locus_tag="VF874_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX9176055.1"
                     /translation="FLATAPLPPWRDVRNAIEHGPIPLQGKSYVGGGRDDPKARDEAC
                     LTLTVWSPDTAASLPVMVWIPGGAFVFGAGQFQLYNGARLAANGDVVVVNVTYRIGVF
                     GGLELSSLGDGFDDNLALRDQIAALQWVRDNVSAFGGDPRRVTVFGDSAGATSVLALL
                     ASPAADGLFGRAIAQSPALPLIADRELRARRAEEFVRELDVGVADIKGLPQRQLRLAA
                     SRLQERSAASTPTLAYGLTHGVDLLPRHPTDAARAAEITRLPLIVGTNSHEATMFAWS
                     KPPMLPTTPALVDDYFRRVAPDAKDHVLAAYPGYPRRRAAIEVASDVMFGAPAWAFAD
                     AYSAVAPTYAYRFDHVGASLRALRLGATHGSEIVHVHHTYDSYLGRLIHPLGRRIQPA
                     VGRRMQRAWLDFAAGAARPATSDSAAPSDDWPRYELPRRATRVIKSTRDVVVDDPDRL
                     RREAWANLH"
BASE COUNT          324 a          741 c          704 g          318 t
ORIGIN      
        1 gattcctggc gaccgcgccg ttgccgccgt ggcgcgacgt gcgtaacgca atcgaacacg
       61 ggccgattcc gctgcagggc aagtcgtatg tcggcggcgg acgcgacgac ccgaaggcgc
      121 gggacgaggc ctgcctgacg ctgacggtgt ggtcacccga cacggcggcg tcgctgccgg
      181 tgatggtgtg gatccccggc ggcgcgttcg tgttcggcgc cggacagttc cagctctaca
      241 acggcgcgcg gctggccgcc aacggcgacg tggtcgtcgt caacgtcacc tatcggatcg
      301 gggtgttcgg cggcctggag ctgtccagtc tcggcgacgg cttcgacgac aacctcgcgc
      361 tgcgcgacca gatcgccgca ctgcagtggg tgcgtgacaa cgtctccgcg ttcggcggcg
      421 acccgcgccg cgtgacggtg ttcggcgact ccgccggtgc gacgtcggtg ctggcgctgc
      481 tggccagccc ggccgccgac ggcctgttcg gccgcgcgat cgcgcagagc cccgcgctgc
      541 cgctgatcgc cgaccgcgag ctgcgggccc gtcgcgccga ggagttcgtc cgcgaactcg
      601 acgtcggcgt cgccgacatc aagggcctgc ctcagcggca gttgcgcctc gccgccagcc
      661 ggcttcagga gcgcagcgcg gcatccacgc ccacgctcgc gtacgggctc acccacggcg
      721 tcgacctgct gccgcggcat ccgaccgacg ccgcccgggc cgcagagatc acccggctgc
      781 cgctgatcgt cggcaccaac agccacgagg cgacgatgtt cgcctggtcg aagccgccga
      841 tgctgcccac gacgcccgcc ctcgtcgacg actacttccg cagggtggcg ccggacgcca
      901 aagaccacgt gctcgccgcc taccccggct atccgcggcg ccgggccgcg atcgaggtgg
      961 cgtccgacgt gatgttcggg gcgccggcct gggcgttcgc ggacgcgtac agcgccgtcg
     1021 cgccgacgta cgcgtaccgc ttcgaccacg tgggggcgag cctgcgggcg ttgaggctcg
     1081 gcgccacgca cggcagcgag atcgtgcacg tgcaccacac ctacgactcg tatctgggcc
     1141 ggctgatcca tccgctcggc cggcggatcc agcccgcggt gggtcggcgc atgcaacggg
     1201 cgtggttgga cttcgcggcc ggagccgctc ggccggcgac atcagattca gcggcaccca
     1261 gcgacgactg gccccgctac gagctgccgc ggcgggcaac gcgggtgatc aagtcgaccc
     1321 gcgacgtcgt cgtcgacgac ccggaccggc tccgccgcga agcgtgggca aacctgcact
     1381 gacccttctt gcctccagac ccacgggcat caccgctgcg ctgtcacccc ctgagaggcg
     1441 catcccccac gcatcgaacg cccgactccc aatagccgtt acctaagtag agacaccctt
     1501 agcgaggagg ccgaaccgcg atgagtccga tattggttgg catgttgtca gtaggcatgc
     1561 ctttgacggc tctcgctctc cacgacctcc agcagtcgct cgaacgcagg gcgcagagcc
     1621 ggcacgccga cgactaggcg ctcctgttgc aagtgttggt ggtgtggccg gtcccgcaac
     1681 aagggcgggt ccggaagtcg caagacggcg taaaatacct tgcgtcacac ccgaatgcgt
     1741 cccgtccgag ggtatcgccg caaggcggcc acctttccaa gtcgggcact tcggacaacg
     1801 tggtccaaga gattggggag tgtgatgatg gtgaccctca aaatgccggc acggctggtc
     1861 ttcgctggtg gaatcgcagt cgcgatcacc gtcagcccgg tggtcgcagc cttggctggt
     1921 ccagctatct cgcccccggt cagcgccgtg gccgaccctg ggcagagctg catcatcaag
     1981 caatccaacg gatctgcgta tctcgactgc gggcctagca tgagaaccgg tcgaacatcg
     2041 gctccgtcga acgtgggcgc gcccagtgaa tcagcgctga ccgccaa
//
LOCUS       DASWBF010000230        23959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1005110,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000230 DASWBF010000000
VERSION     DASWBF010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23959
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005110"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VF874_08165"
     CDS             <1..489
                     /locus_tag="VF874_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX9176056.1"
                     /translation="IARLMKGFATGAIPVQTLAAQLVSTSEAPRSLLRQAVRFAAVGI
                     ASTIAYLLLFIALRHTVGAQTANLLALLLTAVVNTAANRRFTFGVRGGARVARHQLEG
                     LVVFGIALAITSGSLAALHAWASQPHHAIELAVLVVANLTATAVRFVLLRGWVFHPRR
                     TQ"
     gene            524..2362
                     /locus_tag="VF874_08170"
     CDS             524..2362
                     /locus_tag="VF874_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9176057.1"
                     /translation="MTLLDDRVTVTTPAETSARPGRSWERLALFALLGATAVLYLWGL
                     GASGWANNYYAAAVQAGSQDWKAWLFGSLDPGNAITVDKPPAALWVMGLSARIFGFSP
                     FSMLLPEALMGVGAVALLYASVRRCSGPAAGLIAGAVMALTPVAAVMFRYNNPDALLV
                     LLLTAAAYFMLRAIDSGATRWIVLAGTAIGFAFLTKMLQAALVLPAFALVFLLAAPGR
                     TWKRLWQLLIGAAAMAISGGWLIALVNLWPAESRPYIGGSTDNSMLQLALGYNGVQRV
                     AGETGPAGNNNPMFGGSSGIGRLFAQSMGTEVSWLLPAALIGLVAGVWFTARTDRTGR
                     VRAHLLLWGGWLLVSGAVFSFMEGVIHPYYTVALAPAIAALIGISVRELWQGREFFSS
                     RAVLATMSASTGAWAFILLNRTPDWLPALRWIVLVGSIAAAAVMAVGAHRLARAIAAV
                     AAIALLFGLAAPAAYSIDTVARSHSGPVPAAGPGSVAFGPGPGSRPKERATDNAALQP
                     LLTGAQSRWAAATVGSMSASGIQLQTGASIMAIGGFSGSDNSPTLEQFQQYVAGHQVR
                     YFIAGKAEGPRGRNGTASAITAWVEQHFTATDVGGTTVYDLTSPAG"
     gene            complement(2369..4360)
                     /locus_tag="VF874_08175"
     CDS             complement(2369..4360)
                     /locus_tag="VF874_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX9176058.1"
                     /translation="MVLSTESANGVAPAPLSVTGPAPFVDARPSRNPFPPIADYGFLS
                     DCENTCLISSSGSVEWMCVPRPDSPSVFGAVLDRSAGHFRLGPYGVAVPVARRYLPGS
                     LIMETTWQTHTGWLIVRDALVMGPWEDFGTRSRTHRRTPMDWEPEHMLLRTVRCVSGT
                     AELQMSCAPSFDYHRIAATWEYSGSAYRQAVAQAKESPEQHPSLTLTTDLRIGVEGRE
                     ARARTRMSEGDEVFVALSWSRYPPPQTYREATDKMWNTTEYWRQWINIGNFPDHPWRS
                     FIQRSALTLKGLTYAPTGALLAAPTTSLPETPHGERNWDYRYAWVRDSTFALWGLYTL
                     GLDREADDFFSFIAEVSGANDGELDPLQPMYGVGGERTWEEEELHHLSGYDGARPVRI
                     GNAAYNQRQHGIWGGLLDSVYLHAKSRDQISDVLWPVLKSQVEQAIKHWREPDRGIWE
                     VRGEPQQFTSSKVMCWVAVDRGAKLAELEGERASAQQWRAIAEEIKADVLAHGVDSRG
                     VFVQRYGSDMLDASLLLVVLTRFLPPDDPRVRNTVLAIADELTQDGLVLRYRVEETDD
                     GLSGQEGAYTICSFWLVSALVEIGELSRAKRLCERLLSLASPLHLYAEEIDPRTGRHF
                     GNFPQALTHLSLINAVVHVIRAEEQSDTTGGFLPANAPM"
     gene            complement(4401..5141)
                     /gene="sepIVA"
                     /locus_tag="VF874_08180"
     CDS             complement(4401..5141)
                     /gene="sepIVA"
                     /locus_tag="VF874_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepIVA"
                     /protein_id="HEX9176059.1"
                     /translation="MYRVFEALDELGAIVEEARGVPMTAGCVVPRGDVLELVDDIKDA
                     IPGELDDAQDVLDARDTMLREAKEHSESMVSTATAEADSVLNHARTEADRLLSEAKSH
                     ADRMVAEARAHCDQIVAEARDEASRITATAKREYEVMTTRAKSEADRLIENGNISYEK
                     AVQEGIKEQQRLVSQTEIVQSARAEATRMIDAAHAEADRLRGECDIYVDSKLAQFEEY
                     LNGTLRSVGRGRHQLRTAAGTHDYVQHA"
     gene            complement(5208..5693)
                     /gene="coaD"
                     /locus_tag="VF874_08185"
     CDS             complement(5208..5693)
                     /gene="coaD"
                     /locus_tag="VF874_08185"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9176060.1"
                     /translation="MSGAVCPGSFDPVTLGHIDVFERAAAQFDEVIVAVLSNPNKTGM
                     FTVDERIAMIDEACEHLPNLCAEAGEGLVVDFVKSRGLTAIVKGLRTGTDFEYELQMA
                     QMNKHIAGVDTFFVATTPQYSFVSSSLAKEVAGLGGDVSALLPEPVNRRMAAKLRRHT
                     D"
     gene            complement(5718..6269)
                     /gene="rsmD"
                     /locus_tag="VF874_08190"
     CDS             complement(5718..6269)
                     /gene="rsmD"
                     /locus_tag="VF874_08190"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX9176061.1"
                     /translation="MTRIIAGRAGGRRIAVPPKGTRPTSDRVREALFNVLAARLDFVG
                     IDVLDLYAGSGALGLEALSRGAARTTFVEANPPTAAVIERNVATLGLTGATVVARPVA
                     TVLAGGGGPADLVLADPPYDMPVDDVNAMLAALGSSGWSAVGTVVVVERRASSPQLRW
                     SDGWRVWPGRRYGDTRLEIAEVD"
     gene            complement(6266..9649)
                     /locus_tag="VF874_08195"
     CDS             complement(6266..9649)
                     /locus_tag="VF874_08195"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEX9176062.1"
                     /translation="MIHKVLVANRGEIAIRACRAAYELGIATVAVYPYEDRNSVHRLK
                     ADESYQIGEKGHPVRAYLSVDEIVATARRAGADAIYPGYGFLSENPELAEACADAGIT
                     FIGPSAEILELTGNKARAIEAARAAGLPVLNSSAPSTSVDELVEAADEIQFPLFVKAV
                     SGGGGRGMRRVTDPGALPEAIEAASREAESAFGDPTVYLEQAVINPRHIEVQILADTN
                     GNVIHLFERDCSVQRRHQKVIELAPAPNLSDELRQRICADAVAFARQINYSYAGTVEF
                     LLDECGHYVFIEMNPRIQVEHTVTEEITDVDLVGSQMRIADGESLEDLGLQQDSVQVR
                     GAALQCRITTEDPANGFRPDTGRITGYRSTGGAGVRLDGATNLGAEVSAHFDSMLVKL
                     TCRGRDFSAAVARARRALAEFRIRGVSTNIPFLLAVVTDSDFRAGRINTSFIDERPWL
                     VTSRTPADRGTKILNYLADVTVNQPHGPRPATVYPQDKLPSVDLSSLPPPGSKHRLMQ
                     LGPEGFASWLKESAPVGVTETTFRDAHQSLLATRLRTTGLLMVAPYIARMTPQLLSIE
                     CWGGATYDVALRFLKEDPWERLAAIREAVPNICLQMLLRGRNTVGYTPYPESVTHAFV
                     QEATAVGVDIYRIFDALNNVESMRPAIDAVRETGTAIAEVAMSYTGDLSDPVENLYTL
                     DYYLKLAEQIVDAGAHVLAIKDMAGLLRPQAATALVGALRSRFDLPVHVHTHDTPGGQ
                     LATYLAAIHAGASAVDGAAAPLAGTTSQPALSAIVAAAARTEYDSGLSLSAVCELEPY
                     WEALRKVYAPFESGLPSPTGRVYSHEIPGGQLSNLRQQAIALGFGDRFEEIETNYAAA
                     DRVLGRLVKVTPSSKVVGDLALALLGAGITADEFASEPSRLDIPDSVIGFLRGELGDP
                     PGGWPEPLRTKALAGRPPAKPRQELTAEDEKALSNAGPQRQTALNRLLFPRPTKEFEE
                     HRELYGDTSRLSANQFFYGLRQGDEHRVHLERGVELLIGLEAISDPDERGMRTVMCIF
                     NGQLRPVLVRDRSIATSVPAAEKADRSNADHIAAPFAGVVTVAVAEGDEVTAGQTVAT
                     IEAMKMEAAITAPKAGVVARVAVSQTAQVEGGDLLMVVS"
     gene            complement(9649..10290)
                     /locus_tag="VF874_08200"
     CDS             complement(9649..10290)
                     /locus_tag="VF874_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX9176063.1"
                     /translation="MTVEAATPAPAADGSAEHGPRAAGVTVPRASAWWVLIAGVVGLA
                     ASLTLTIEKVALLKDPSYRPTCSINPVLSCGSVMVTPQASVFGFPNPLMGIVAFSVVL
                     VTGVLAVARVALPRWYWLGLAAGTLLGGAFVHWLIFQSLYRINALCPYCMVVWSVTIP
                     LFVVVSSIALQPLEKYAAARVVYRLRWSLAALWFSAVILMILVQFWDYWSTLI"
     gene            complement(10287..11087)
                     /locus_tag="VF874_08205"
     CDS             complement(10287..11087)
                     /locus_tag="VF874_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9176064.1"
                     /translation="MANKKKGYDLNAADRRRNLLIQVGLTAVVILFAVGLLLLILKPW
                     EPKPTKPSVTGPPAGTVMPITVAVPDKLAKDAAGKPKVVLSMFEDFLCPACGAFERNF
                     GLTVKQLIDTGAVQADYYMVALLDSPQRQFYSSRAGSAAYCVAEFDTSPTKESFQKFH
                     SALYANQPDEVTGPFPTDDDLVQRARDAGAAGTNVSDCILGDKYLDMVRGLVRATGLK
                     GTPTIKINNQEVDMSTVHSPQDLINKVNEASAAAPASPAPAPVPAPAP"
     gene            complement(11188..12189)
                     /locus_tag="VF874_08210"
     CDS             complement(11188..12189)
                     /locus_tag="VF874_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9176065.1"
                     /translation="MPKVPDAAKRFMTRSITIDGNTLDPTMQLILAGQKLSGEEGLSA
                     ADDPETCRRNVHQFTQMVDRKRLPVGAVSDLSIPGPAGPIPTRHYRPANDEVGPLLVY
                     FHGGGYVIGDLDTVDNLCRMICRGAGIHVLSVDYRLAPEHPAPAAVEDCYAAYRWALE
                     HGPELGTRPGVVAVGGDSAGGALSAVVSRLGRDDGLPPALQLLLYPWTDQRGGTRSRA
                     LFARGFLLTARDLDWFRGHYLANSELDVGDPLVSPVLAPELSGLPRALVITAGFDPLR
                     DEGEAYAVAMRDAGVAVDLRRMCTLVHGFTNLWPLGGDSAFAMAEIVSALRAHLCYI"
     gene            12381..13226
                     /locus_tag="VF874_08215"
     CDS             12381..13226
                     /locus_tag="VF874_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9176066.1"
                     /translation="MNAPSITLNDGNAIPGVGLGVFKTPPKETEQAVRAALDAGYRHI
                     DTASAYRNEREVGRAIAKSPTPRDEIFVVTKLWNADQGYDATMKAFEKSMKRLGLDRD
                     GDYLDLYLIHWPVPAISEFVDTWRAFAALHDLGRIRSIGVSNFAPEHLNTLIDATGVV
                     PVVNQIELHPLMTQGELREMHAQLGIATEAWSPLGRGRLLDHPTISSIADAYGKTPAQ
                     AIIRWHLQIGNIVIPKSVNPERIASNFDVFDFELSDDDVASISSLDNGTRLGPDPRTF
                     NFTGR"
     gene            13228..14052
                     /locus_tag="VF874_08220"
     CDS             13228..14052
                     /locus_tag="VF874_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9176067.1"
                     /translation="MTPTVTLNDDNVIPVLGLGVAELSEEDTERAVSAALEAGYRLID
                     TASVYGNEAAVGRAIAASGLPRDELFVTTKVANADQGFNSTKEALAASLERLGLEYVD
                     MYMIHWPAAQLGKYIDTYGSMQKIQQEGQIRSIGVCNFLGEHLSTISDLTYTNPAVNQ
                     IELHPLLNQAELRDLHADRGIVTEAYSPLGVGNVLENPTLVSIAQAHGKSPAQVAIRW
                     SIQLGNVVIPRSGNPERIKENIDVFGFELTDDEMSQINGLDNGTRFREDPATYTGT"
     gene            complement(14130..14843)
                     /locus_tag="VF874_08225"
     CDS             complement(14130..14843)
                     /locus_tag="VF874_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HEX9176068.1"
                     /translation="MRRRTWMWLVAAVVVTLLVAYQTGTSAWRHRSDAFVAQADVPTV
                     APGVDVLSGIPVIPLRIRGYDYRRAAFGDSWTDDTDAPGGHNGCDTRNDILDRDLVDK
                     TYTSISRCPRAVATGTLHDPYTNAMIAFLRGEQTGASVQIDHIVPLALAWDLGARGWT
                     DELRERFANDPANLLAVDGPANQDKGDQGPSTWMPPNEAFWCQYAVQFAAVLRGYGLP
                     VDQASADRLRDAAATCPTG"
     gene            complement(14840..17068)
                     /gene="recG"
                     /locus_tag="VF874_08230"
     CDS             complement(14840..17068)
                     /gene="recG"
                     /locus_tag="VF874_08230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX9176069.1"
                     /translation="MATLGDRLDFVLGRTTAKKLEENFGIRTVNDLLRHYPRKYSEGM
                     TVRGGDEDLDVEEGEHVTFVDVITDTKSDYMKPRPDKRKRKWLRVTLGNHRPKVTATF
                     FNADWMIGKLVPDTRIMLSGEVKYFGRTMQLTHPAFLVLHSPSGDKPGTKSLKTIATA
                     SGAAGEDELLAVFEREFFPIYPASAKVQSWDIYACVRQVLAMLDPIEDPLPESLLRQR
                     NLISEDEALRAIHLAENATERDRAQNRLTFDEAVGLQWALVARRYGELGESGPPAPRR
                     DDGLVAALLRQLPFELTDGQRDVLDVLSTELDRTRPMNRMLQGEVGSGKTIVSVLAML
                     QMVDAGYQCALLAPTEVLAAQHARSVRDILGPLAMAGQLGGAEGATAVALLTGSMSAQ
                     QKRHVRDEVASGEAGIVIGTHALLQDAVEFKQLGMVVVDEQHRFGVEQRDRLRAKAPD
                     DLTPHLLVMTATPIPRTVALTVYGDLETSTLRELPRGRQPITTNTIFVQDKPQWLDRA
                     WQRITEEVAAGRQAYVVASRIDETDNPVSDAGGPPATTVLELYDRLRRGPLSRLRVGL
                     MHGRLSADEKDAVMAAFRGGQIDVLVCTTVIEVGVDVPNATVMFVMDADRFGISQLHQ
                     LRGRIGRGQHPSLCLLATRLPEGSNAGQRLKAVAGTLDGFKLADLDLQERREGDVLGH
                     SQSGRPITLRFLSLSEHQEVIEAAREFCETAYERNPNQPGMAVLAAQFTETDRVEYLD
                     KS"
     gene            complement(17071..18711)
                     /locus_tag="VF874_08235"
     CDS             complement(17071..18711)
                     /locus_tag="VF874_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX9176070.1"
                     /translation="MSGRRLDASGLLDWAHAAVGGLVVHAEEINRLNVFPVADADTGT
                     NMLFTMRAALAEAERHAKRSGDVSQVAAALARGALRGARGNSGVILSQILRGLADVTA
                     SASADTDSHLADVDAVLFGAALRHGVALAVGSMGEEVAGTIVSVLQAAAARAEHCADD
                     GDDLASTVLSAAEAAVTALERTPEQLGVLAEFGVVDAGGRGLVVLLDALAATLTGHTP
                     ARGEYVPSPPVQDATEPSADVRPPHYEVMYQISATGQRLDALRERLEELGDSVAIAGD
                     GGDRYSVHVHADDAGAAIEAGLAVGTPSQIQISTLHGAVMHSRWTRRRVVLAVVDGDG
                     AEQLFTSEGAHVLRCADDRPDPSASISAKELLRAVVDTGAGQVVVLPNGFVAAEELVA
                     GCAAATGWGLSVVPVPTGSMVQGLAALAVHEPGRAVVDDGYSMTRAAGATRHASVRVA
                     DEEALTFAGPCEPGDGLGIAGDEVLVVRHDIAATARRLLDLLLGAGGELVTVLVGAGV
                     DDWVGEDLQAHVHRHHPGTELVTYKTGHRGDALLIGVE"
     gene            18904..19098
                     /gene="rpmB"
                     /locus_tag="VF874_08240"
     CDS             18904..19098
                     /gene="rpmB"
                     /locus_tag="VF874_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009030042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX9176071.1"
                     /translation="MAAVCDICGKGPGFGKNVSHSHRRTNRRWNPNIQTVRAVARPGG
                     NKQRLNVCTSCLKAGKVARG"
     gene            complement(19180..19869)
                     /locus_tag="VF874_08245"
     CDS             complement(19180..19869)
                     /locus_tag="VF874_08245"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9176072.1"
                     /translation="MTTRPLKELVDDGWAQALEPVADQVAAMGEFLRAELAAGQRYLP
                     AGRNVLRAFTFPFEKVRVLVVGQDPYPTPGHAVGLSFSVAPDVRPLPRSLDNIFAEYC
                     TDLGYPTPSNGDLTPWAERGVLLLNRVLTVRPQNPASHRGKGWEVVTECAIRALADRP
                     QPLVAILWGRDAATLKPMLAEGGSRVATIDSVHPSPLSAQRGFFGSRPFSRANALLSD
                     LDAEPVDWRLS"
     gene            complement(19880..20170)
                     /locus_tag="VF874_08250"
     CDS             complement(19880..20170)
                     /locus_tag="VF874_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX9176073.1"
                     /translation="MTVYAIAQLKFTDRAAYDRYQARFMEVFRRHPGTLLAADESPQV
                     IEGEWNLDKVVLVSFPDEAAFRAWAESPEYRSISEDRHAGADTVVLLVNGLA"
     gene            complement(20180..21133)
                     /locus_tag="VF874_08255"
     CDS             complement(20180..21133)
                     /locus_tag="VF874_08255"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9176074.1"
                     /translation="MSKEPTLADLGEFAVIDRLLAGRRHGAHTLLGPGDDAAVVAAPD
                     GRVVVSTDMLVESRHFRRDWSTPHDIGRKAMAQNAADVEAMGAAATGFVVSFGAPADT
                     PVAQVGELADGMWDEARRVGSGIVGGDLVSSPQWVVSVTVLGDLAGRAPVTRSGATAG
                     SVIAVCGELGRSAAGYSLWQLNFPGFDDLRRRHLVPEPPYGQGVLAAKAGARAMTDIS
                     DGLVADVRHIAEASGVGIELSTAGLAGDRDAVLEAAQVVGADAWAWVLGGGEDHALVA
                     VFDGRVPHGWRVIGAVVDGPPRVLVDSAEWQGHAGWQSFGS"
     gene            21230..21472
                     /locus_tag="VF874_08260"
     CDS             21230..21472
                     /locus_tag="VF874_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9176075.1"
                     /translation="MVEAFMLIQTEVGRAEVVAKQLATLAGVLTAEYVTGPYDVIVRI
                     GADTLERLQADVVPSVQQTAGITRTLTCPIAGKSQP"
     gene            21442..22002
                     /locus_tag="VF874_08265"
     CDS             21442..22002
                     /locus_tag="VF874_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 domain-containing protein"
                     /protein_id="HEX9176076.1"
                     /translation="MPDRRQVPALSDGPPRALLVAAVGVAVAAAVAVLVIAVTTSRQP
                     QERPLVVPAVPVPQADAPECATLMGALPQRLGDYQRATPADPVPPGTAAWRSGSDDEP
                     ILLRCGVGQPTDFVVGAQINVVNTVQWFRVPEQSTGRSTWFVVDRPVYVALTLPQGTG
                     ATPIQEMSDVVAAKLPAVPIRPGPPR"
     gene            complement(22006..23061)
                     /locus_tag="VF874_08270"
     CDS             complement(22006..23061)
                     /locus_tag="VF874_08270"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX9176077.1"
                     /translation="MTAGRVRVAVVYGGRSNEHAISCVTAGSILRHLDPARFDAVPIG
                     ITREGTWVLTNADPGNLAIEGGRLPEVTASSGAELALGEALASVDVVFPVLHGPYGED
                     GTIQGLLELAGVPYVGAGVFASAAGMDKEFTKKLLAAERLPIGDHTVLRPRQATLTER
                     DKNRLGLPVFVKPARAGSSIGVSRVSSWEELPAAIENARQHDPKVLVEKAIRGRELEC
                     GVLELPDGTVQASTVGEIRVEGVRGREDAFYDFATKYLDEGAELDVPAKVDDEVAEDV
                     RALAIRAFHAIDCQGLARVDFFLTENGPLVNEINTMPGFTTISMYPRMWAASGVDYPT
                     LLAAMVDTALARGVGLR"
     gene            complement(23081..>23959)
                     /locus_tag="VF874_08275"
     CDS             complement(23081..>23959)
                     /locus_tag="VF874_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX9176078.1"
                     /translation="YGRSSNPTWRQLESAIAQLEGATAALVFGSGMAAITSVLRALVR
                     PRGVLVMPADGYYQVRRYAVEYLARQGITVREATAGQMCEAAADADVVLAETPVNPQL
                     DVVELHQLALMCRGRGATLVVDNTAATPLGQQPLGLGADLVVASATKALAGHSDLVAG
                     YVAGSKPELIAAVAAERLLTGPILGAFEAWLVLRSLGTAGLRLERQCRNAQALATMLH
                     SHPAVRSVRYPGLPDDPAYPTASAQMQRFGGIHTSVDGRARWGDQVVDGFARLSAGIE
                     DTDDLVADVESALSAV"
BASE COUNT         3858 a         8308 c         7881 g         3912 t
ORIGIN      
        1 atcgcccggc tgatgaaagg tttcgccacc ggcgcgattc cggtgcagac gctggctgca
       61 cagctcgttt cgacgtcgga ggcgccgcgc tcgctgctgc gccaagccgt gcggttcgcc
      121 gcggtcggca tcgccagcac gatcgcctac ctgctgctgt tcatcgcatt gcggcacacc
      181 gtcggcgcgc agaccgcgaa cctgctcgcg ctgctgctga cggcggttgt caacaccgcc
      241 gccaatcgcc gcttcacgtt cggcgtgcgc ggcggtgccc gcgtcgctcg ccaccagctc
      301 gaggggctcg tcgtgttcgg tatcgccctc gccatcacca gcggctcact ggcagcgctg
      361 cacgcctggg cgagccaacc ccatcacgcg atagagctcg ccgtgctcgt cgtcgccaac
      421 ctcaccgcaa ccgctgtccg cttcgtcctg ttgcgcggct gggtgttcca tccgcggcgc
      481 acgcagtgac cctcgcccac aaccactttc ggagaccaaa tccatgactc tgctcgacga
      541 ccgcgtcacc gtcaccacac cggcggaaac ctcggcacgc cccggccgca gttgggaacg
      601 cctagcgctg ttcgcgcttc tgggcgcgac cgccgtcctg tacctgtggg ggctcggcgc
      661 gtccggttgg gccaacaact actacgccgc cgccgtgcag gccggcagcc aagattggaa
      721 ggcgtggctg ttcggatcgc tggaccccgg caacgcgatc accgtcgaca aaccgcccgc
      781 cgcgctgtgg gtgatggggt tgtcggcgag gatctttggc ttcagcccat tctcgatgct
      841 gctgccggag gcgctgatgg gggttggcgc cgtcgcgctc ctgtacgcgt cggtgcggcg
      901 ctgcagcggt ccggccgcgg gcctgatcgc cggagcggtg atggcgctga cgcccgtcgc
      961 ggcggtgatg ttccgctaca acaatcccga cgcgctgctc gtgctgttgc tgacggccgc
     1021 tgcgtatttc atgctgcgtg cgatcgattc cggcgccacc cgctggatcg tgctggctgg
     1081 taccgcaatt gggttcgcat tcttgacgaa aatgctgcag gccgccctgg tgctgccggc
     1141 cttcgcgctg gtgttcctgc tcgccgcccc cggccggacg tggaagcggc tgtggcagct
     1201 gctgatcggt gcggccgcaa tggcaatttc gggaggctgg ctgatcgcgc tggtgaacct
     1261 gtggccggcg gaatcccggc cctacatcgg cggctcgacc gacaacagca tgttgcagct
     1321 cgcgctgggt tacaacggcg tgcagcgcgt cgctggcgag accgggccag ccggcaacaa
     1381 caatccgatg ttcggcggct ccagcggcat cggccgcttg ttcgcgcagt cgatgggcac
     1441 ggaggtgtcg tggctgctac cggccgcgct aatcggtctg gtcgccggtg tctggttcac
     1501 cgcacgtacg gaccgcaccg gtcgtgtgcg ggcccatctt ctgctgtggg gcggctggct
     1561 gctggtcagc ggcgcggtgt tctcgttcat ggagggcgtc atccacccgt actacaccgt
     1621 ggcgctggcg ccggcgatcg ccgcgctgat cggcatctcg gtccgcgaat tgtggcaggg
     1681 ccgcgaattc ttcagcagca gagcggtatt ggcgacaatg tccgcgtcta ccggtgcgtg
     1741 ggcgttcatc ctgttgaacc gcacaccgga ctggctgccg gcgctgcgct ggatcgtgtt
     1801 ggtcggatcg attgccgccg ctgccgtgat ggctgtgggt gcgcaccggc tggctcgcgc
     1861 gatcgctgcg gtcgccgcca tcgcgctact gttcggcctc gccgcaccgg cagcctattc
     1921 gatcgacacg gtcgcccggt cacacagtgg gccggtgccc gcggcaggac ccggcagcgt
     1981 cgcgttcggc cccggaccag gaagtcggcc gaaggaacgc gccaccgaca acgccgcgct
     2041 gcagccattg ctgacgggcg cccaaagccg ttgggccgcg gcaaccgtcg ggtccatgtc
     2101 ggcgagcggg atccagctgc agaccggtgc ttcgatcatg gcgatcggcg gcttcagcgg
     2161 atcggacaac tcgcccacgc tggagcagtt ccagcagtat gtcgcgggcc accaggttcg
     2221 ctacttcatc gcaggcaagg cggaaggccc gcgagggcgc aacggcaccg caagcgcgat
     2281 caccgcctgg gtggagcaac atttcaccgc cacggacgtc ggtggaacga ctgtgtacga
     2341 cctgacctca ccagccgggt gagtgctgtc acatcggggc gttggccggc aggaatcctc
     2401 cggtggtgtc ggactgctcc tcggcgcgga ttacgtgcac caccgcgttg atcagcgaca
     2461 gatgggtgag cgcttgtggg aagttgccga agtgtcgccc ggtgcgtggg tcgatctcct
     2521 cggcgtacag gtgcagcgga ctggccagcg acagcagccg ttcacagagc cgtttggcac
     2581 gcgacagttc gccgatttcc accagggcgg acaccagcca gaacgagcag atcgtgtacg
     2641 cgccctcctg cccggacagg ccgtcgtcgg tctcctccac gcgatagcgc agcaccaacc
     2701 cgtcctgcgt caactcgtcg gcgatcgcca gcaccgtgtt gcgcacccgt gggtcgtcgg
     2761 gcggaaggaa gcgggtgagc accaccaaca gcaacgacgc gtccaacatg tccgagccgt
     2821 accgttgcac gaacactccg cgcgagtcga caccatgggc caacacgtcg gccttgatct
     2881 cctcggcgat cgcccgccac tgctgcgcgc tggccctctc tccctccagt tcggcgagct
     2941 tggcgccgcg gtccaccgcc acccagcaca tcaccttcga cgacgtgaac tgctgcggct
     3001 ctccgcgcac ctcccagatg ccgcggtcgg gttcgcgcca gtgcttgatc gcctgttcga
     3061 cttggctttt cagcaccggc cacaagacgt ccgaaatctg gtcccgcgac ttggcgtgaa
     3121 ggtacaccga atccagcagg cctccccaaa taccatgctg ccgttggtta tacgcggcat
     3181 tgccgatccg cacgggccgg gcgccgtcgt agccggagag gtgatgcagt tcctcttcct
     3241 cccaggtgcg ctcgcccccg acgccgtaca tcggctgcag cggatcgagt tcgccgtcgt
     3301 tggcgccgga cacctcggcg atgaacgaaa agaagtcgtc ggcctcgcga tccagaccca
     3361 aggtgtaaag cccccacagc gcgaacgtgg agtcgcgcac ccatgcgtag cggtagtccc
     3421 agttacgttc tccgtgaggc gtttccggca gcgacgtggt gggggcggcc aacagcgcac
     3481 cggtgggcgc atatgtcaag cccttcaatg tcaacgcact gcgctggatg aacgaccgcc
     3541 acggatggtc tgggaaattc ccaatgttga tccattgccg ccagtactcg gtggtgttcc
     3601 acatcttgtc ggttgcttcc cggtaggtct gcggcggcgg atatctcgac cagctgagcg
     3661 cgacgaacac ctcgtcaccc tcgctcatcc gggttcgcgc ccgcgcctca cgaccttcca
     3721 caccgatccg cagatccgtc gtcagcgtga gactcgggtg ctgttccgga ctctccttgg
     3781 cctgcgcgac cgcctgccga tacgccgaac cggagtattc ccacgtcgcc gcgatccggt
     3841 gatagtcgaa cgacggagcg cagctcatct gcagctcggc ggtgccgctg acgcagcgca
     3901 ccgtgcgcag cagcatgtgc tccggctccc agtccatcgg cgtgcgacgg tgcgtccgcg
     3961 accgcgtccc gaagtcctcc cacggtccca tcaccaatgc gtcgcgcacg atcagccaac
     4021 cggtgtgcgt ctgccaggtg gtctccatga tcaggctgcc cggcagatac cgccgcgcca
     4081 ccggcaccgc cactccgtat ggcccgagcc ggaaatgacc ggccgagcgg tccaagacgg
     4141 cgccgaacac actcggcgag tctggccgtg gcacgcacat ccactcaacg gaaccgctcg
     4201 aagaaatcag gcaggtgttc tcgcagtccg acaagaaccc gtaatccgcg atcggcggaa
     4261 acggatttcg gctgggcctg gcgtcgacaa acggcgccgg ccccgtcacc gacagcggcg
     4321 caggagccac cccgttcgcc gactccgtac tgagcaccat ctgctcatca tgctcagcgc
     4381 aaaggcgaag tcatacccct tcaggcgtgt tgcacgtagt cgtgcgtgcc tgcggcggtg
     4441 cgcagctgat gacgtccccg cccgacggag cgcagcgtgc cgttcaggta ctcctcgaac
     4501 tgcgccagct tgctgtcgac gtagatgtcg cactcgcccc gcagccggtc cgcctcggcg
     4561 tgcgccgcgt cgatcatccg ggtcgcctcg gcccgcgccg actgcacgat ctcggtttgc
     4621 gacaccagcc gctgctgttc cttgatgccc tcctggacgg ccttctcgta ggagatgttg
     4681 ccgttctcga tcaggcggtc ggcctcgctc ttggcccgag tcgtcatgac ctcgtactcg
     4741 cgcttggcgg tcgcggtgat gcggctggcc tcgtcgcgcg cctcggcgac gatttgatcg
     4801 cagtgcgccc tcgcctcggc gaccatgcgg tcggcgtgtg atttcgcctc cgacaacagg
     4861 cggtcggcct cggtgcgcgc atggttcagc accgagtcgg cctctgcggt ggccgtcgac
     4921 accatcgact cggagtgttc cttggcttcg cgcagcattg tgtcgcgggc gtcgagcacg
     4981 tcctgggcgt cgtcgagctc gccggggatc gcgtccttga tgtcgtcgac caactccagt
     5041 acgtcgcctc gcggcaccac acagccggca gtcatcggca caccgcgcgc ctcttcgacg
     5101 atcgcgccca gctcatccag cgcctcaaat actcggtaca cggcgatacc ctccagccct
     5161 agtcgttgtg accagtgtgc ctggtgttac gcctgtgact ggggaggtca gtcggtgtgt
     5221 cggcgcagct tggccgccat ccgccgattc accggttcgg gcagcaacgc agacacgtcg
     5281 ccgcccaggc cagcgacctc tttggccagt gacgacgaca cgaacgagta ctgcggtgtg
     5341 gtcgccacga agaaggtgtc cacgccggcg atgtgcttgt tcatctgggc catctgcagc
     5401 tcgtattcga agtcggtgcc ggtgcgcagg cccttgacga tcgcggtcag cccgcgggac
     5461 ttgacgaagt cgacgacgag cccttccccc gcctccgcgc acaggttcgg caggtgctcg
     5521 catgcctcgt cgatcatcgc gatcctctcg tcgacggtga acatgcccgt cttgttcgga
     5581 ttggacagca ccgcgacgat cacctcgtcg aactgggcgg cggcccgctc gaacacgtcg
     5641 atatgcccga gcgtcaccgg gtcgaatgac ccaggacaca ccgcgccgct catggagcac
     5701 gacgctatca gccgacccta atcgacttct gcgatctcca gccgggtgtc gccgtaacgc
     5761 cggcctggcc agacgcgcca cccgtcggac cacctcagct ggggcgacga tgcgcgccgc
     5821 tccaccacga cgacggtccc gaccgcgctc caaccgctcg agcccagcgc cgccaacatg
     5881 gcgttcacgt cgtcgaccgg catgtcgtac ggcgggtctg cgagcaccag atccgccggg
     5941 ccgccacccc cggccagcac cgttgccacc ggacgggcca ccacggtcgc acccgtgagg
     6001 cccagcgtcg cgacattgcg ttcgatcacc gccgccgtcg gcgggttggc ctccacgaac
     6061 gtcgtccgcg ccgctccccg tgacaacgcc tcgagcccca gcgcacccga tcccgcgtag
     6121 agatcgagca cgtcgatgcc cacgaagtcc agccgggcgg ctaacacgtt gaacagcgcc
     6181 tcgcgcaccc ggtcgctggt gggccgggtg cccttcggcg ggaccgcaat gcggcggccc
     6241 ccggcgcgtc ccgcgatgat ccgggtcagc tgaccaccat caacaggtcg ccgccctcca
     6301 cctgcgcggt ctgcgacacc gcgaccctgg ccacgacgcc cgccttgggc gcggtgatcg
     6361 cggcctccat cttcatcgcc tcgatcgtcg cgacggtctg gccggcggtc acctcgtcgc
     6421 cttcggcgac ggcgaccgtg acgacgcccg cgaacggcgc ggcgatgtgg tcggcgttgg
     6481 accggtcggc cttctcggcc gcgggcacgc tcgtggcgat gctgcggtcg cgcaccagca
     6541 ccggacgcag ttgcccgttg aagatgcaca tcaccgtgcg cattccccgc tcgtcgggat
     6601 cggaaatggc ctcgagcccg atcagcaact ccacgccgcg ctcgaggtgc acgcggtgtt
     6661 cgtcgccctg gcgaagcccg tagaagaact ggttggccga cagccgggat gtgtcgccgt
     6721 acagttcgcg gtgttcttcg aattcctttg tggggcgggg gaacagcagc ctgttgagag
     6781 cggtctgacg ttgcgggccc gcgttggaca gcgccttctc gtcctcggcg gtcagctcct
     6841 gccggggttt ggcgggcgga cggccggcga gcgctttggt gcgcagcggc tccggccagc
     6901 cgccgggcgg gtcgcccaac tcgccgcgta ggaacccgat gaccgagtcg ggaatgtcca
     6961 aacgtgatgg ttccgaggcg aattcgtcgg cggtgatgcc cgctccgagc agcgccagcg
     7021 ccaaatcgcc gacaactttc gacgacggcg tcaccttgac cagccgcccg agaacccggt
     7081 cggccgcagc gtaattggtt tcgatctcct cgaaccggtc gccgaagccc aacgcgatcg
     7141 cctgttgccg cagattcgac agctggccgc cgggaatttc gtggctgtac acccggccgg
     7201 tcggcgaggg aagcccggat tcgaacggcg cgtacacctt tcgcagcgcc tcccagtacg
     7261 gctcgagctc gcacaccgcc gacagcgaca acccggagtc gtactcggtg cgcgcggccg
     7321 cggcgacgat cgccgacagt gcgggttgac tggtggtgcc tgccagcgga gcggcggccc
     7381 cgtcgaccgc gctcgcgccg gcgtggatcg ccgccaagta ggtggccaac tggccaccgg
     7441 gggtgtcgtg ggtgtgcacg tgcaccggca ggtcgaaccg gctgcgcagc gcgcccacca
     7501 aagctgtcgc ggcctgcggt cgtagcaggc cggccatgtc tttgatcgcc agcacgtgcg
     7561 cgccggcatc gacgatctgt tcggccagct tcaggtagta gtccagtgtg tacaggtttt
     7621 cgacggggtc agacaggtcg ccggtgtagc tcatcgcgac ttcggcgatg gcggtaccgg
     7681 tttcgcggac cgcgtcgatc gccgggcgca tcgactcgac gttgttcagc gcgtcgaaga
     7741 tgcggtagat gtcgaccccg acagccgtgg cctcctggac gaacgcatgg gtgacggact
     7801 cggggtacgg cgtgtagccg acggtgttgc ggccgcgcaa gagcatctgc aggcagatgt
     7861 tgggcaccgc ttcgcggatc gcggccagcc gctcccacgg gtcctccttc aagaaccgaa
     7921 gcgccacatc gtatgtcgca ccgccccagc attcgatcga cagcagttgc ggcgtcatcc
     7981 gggcgatgta cggcgcgacc atcagcaggc ccgtggtgcg cagccgggtg gccagcagcg
     8041 actggtgcgc atcgcggaag gtggtctcgg taacaccaac gggtgcagac tctttcagcc
     8101 agctcgcgaa tccctcggga cccagctgca tcaaccggtg cttggagccg ggaggtggca
     8161 gcgaggacag atccaccgat ggcagtttgt cttgcggata caccgtcgcc ggacgcgggc
     8221 cgtgcggctg gttcacggtg acgtcggcga gatagttcaa gatcttcgtg ccgcgatcgg
     8281 ccggcgtgcg cgaggtcacc aaccacggcc gctcgtcgat gaaagaggtg ttgatccgcc
     8341 cggcgcggaa gtccgaatcc gtcaccaccg caagcagaaa cgggatattc gtcgacacgc
     8401 cgcggatgcg gaactcggcg agcgcgcgcc gggcccgagc cacggcggcg gagaagtcgc
     8461 gtccgcggca ggtcagcttg accagcatcg agtcgaagtg cgcgctgacc tccgccccca
     8521 ggttcgtcgc gccgtccagc cgaacgccgg caccaccggt cgagcggtag ccggtgatgc
     8581 gaccggtgtc ggggcggaat ccgttggccg ggtcctcggt ggtgatcctg cattgcaacg
     8641 cagcgccacg aacctgcacc gaatcctgct gcaggccaag gtcttcgagc gactcgccgt
     8701 cggcgatacg catctgactg ccgaccaggt cgacgtcggt gatctcctcg gtgacagtgt
     8761 gctcgacctg gatgcgcggg ttcatctcga tgaagacgta gtgaccgcac tcgtcgagca
     8821 ggaactcgac ggtgcccgcg taggagtagt tgatctgtcg ggcgaacgcg actgcatcgg
     8881 cgcaaatgcg ttgccgcagt tcgtcggaca agttgggcgc cggggccagt tcgatgacct
     8941 tctggtgccg gcgctgcacg ctgcagtcgc gttcgaagag gtggatgacg ttgccgttcg
     9001 tgtcggcaag gatctgcacc tcgatgtgac ggggattgat caccgcctgt tcgagataca
     9061 cggtggggtc gccgaacgcc gactctgctt cccggctggc ggcctcgatc gcctcgggca
     9121 gcgcgccggg gtctgtcacg cgccgcatcc cgcggccgcc gccgccggac accgccttga
     9181 cgaacagcgg gaactgtatt tcatctgcag cctcgacgag ctcgtccacc gacgtcgacg
     9241 gcgcagagga attcaggacc ggcagtcccg cggcgcgggc cgcctcgatt gctcgcgcct
     9301 tgttgccggt gagctccagg atctcggcgc tgggtccgat gaacgtgatg cccgcgtcgg
     9361 cacaggcctc ggccagttcc gggttctcgg acaggaagcc gtatccgggg tagatcgcat
     9421 cggcgccggc ccggcgcgcg gtcgcgacga tctcgtcgac agacaggtac gcccgtaccg
     9481 gatggccctt ctcgccgatc tgatacgact cgtcggcctt gagccggtgc accgagttgc
     9541 ggtcctcgta cggatacacc gcgacggtcg cgatgcccag ttcgtaggcg gcgcggcacg
     9601 cgcggatcgc gatttcgcct cggttggcga cgaggacctt gtggatcact agataagggt
     9661 cgaccagtaa tcccagaact gcaccaggat catcagaatc actgcgctga accacagcgc
     9721 cgccagcgac caccgcaacc ggtacaccac gcgggcagcc gcatacttct caagaggctg
     9781 taacgcgatc gaggacacga cgacgaacag cggaatggtc accgaccaca ccaccatgca
     9841 gtacgggcag agcgcgttga ttctgtagag gctctgaaag atcagccagt gcacgaacgc
     9901 gccaccaagc agcgtgccgg cggccagccc gagccagtac cagcggggca gcgcgacgcg
     9961 tgcaaccgcg agcacaccgg tgacgagcac gacgctgaat gcgacgatcc ccatcagcgg
    10021 gttgggaaag ccgaacaccg acgcctgggg ggtgaccatc accgaaccgc aggacagcac
    10081 cgggttgatg ctgcacgtcg gccgatacga cgggtccttc agcagcgcga ccttctcgat
    10141 cgtcagcgtc agcgacgccg ccagcccgac gacgccggcg atcagcaccc accacgcgct
    10201 cgcgcgcggc accgtgacgc cggcggcacg tgggccgtgc tcggccgagc cgtcggcggc
    10261 aggcgccggg gtggccgcct ctacggtcat ggtgcgggcg ccggcaccgg cgccggggca
    10321 ggcgaagcag gagcggcggc ggaggcctcg ttgaccttat tgatcagatc ctgcggactg
    10381 tggaccgtcg acatgtcgac ctcttggttg ttgatcttga tcgtcggcgt gcctttcagg
    10441 cctgtggccc gcaccaagcc cctgaccatg tcgaggtact tgtcgcccag gatgcagtcg
    10501 ctgacgttgg tgcccgcggc gccggcgtca cgtgcccgct gaacgaggtc gtcatccgtc
    10561 ggaaaaggcc cggtcacctc gtcgggttga ttggcgtaca acgcggaatg gaacttctgg
    10621 aacgactctt tggtgggtga tgtgtcgaat tcggcgacac agtaggcagc actgccggcg
    10681 cgtgaggagt agaactgacg ctgcggactg tccaggagag cgaccatgta atagtcggcc
    10741 tggaccgcgc cggtgtcgat gagttgctta accgtgagac cgaagtttct ttcgaacgcg
    10801 ccgcaagccg gacagaggaa gtcttcgaac atcgacagca cgaccttcgg cttgcccgca
    10861 gcgtctttgg ccagcttgtc gggcaccgcc acggtgatgg gcatcacggt gccggcgggc
    10921 ggaccagtta cggacggctt cgtgggcttg ggctcccacg gtttgaggat cagaagcagc
    10981 aggccgacgg cgaagaggat gacgaccgcg gtcagtccca cctggatcaa caggttgcgt
    11041 cgacggtcgg ccgcgttgag gtcgtagccc ttctttttgt tcgccacggg ctcagactac
    11101 cggcggacca attgcacgcc tgatcacacg gcgatgagcg cttgcgcgaa gagcatctga
    11161 ggtcgatgag cgcttgcgcg aagagcatca gatgtagcac agatgcgccc gcagcgcgga
    11221 gacgatctcc gccatcgcga acgcgctgtc cccgcccagc ggccagaggt tcgtgaaacc
    11281 gtggacgagc gtgcacatcc ggcgcaggtc tacggccacg ccggcgtcgc gcatcgcgac
    11341 ggcataggct tcgccctcgt cgcgcagcgg gtcgaaaccg gcggtgatca cgagcgcgcg
    11401 gggcaggccc gaaagctccg gtgcgagcac cggcgacacc aggggatcgc cgacgtccaa
    11461 ctccgagttt gcgaggtagt ggccacggaa ccagtccaag tcacgcgcag tcagcaggaa
    11521 cccgcgcgcg aagagtgccc gggaccgcgt gccgccacgc tggtcggtcc acggatagag
    11581 cagaagctga agcgcgggcg gcaggccgtc gtcgcgcccg agccgggaca ccaccgccga
    11641 cagggcgccg cccgcgctgt cgccgccgac agcgaccacg cccggtctcg taccgagctc
    11701 aggcccgtgc tccagcgccc accgatacgc ggcgtagcag tcctcgaccg cggccggcgc
    11761 gggatgctcc ggcgccagcc ggtagtccac cgacaagacg tggatgccgg caccgcggca
    11821 aatcatccgg cagaggttgt cgaccgtgtc gaggtcgccg atcacatatc cgccgccgtg
    11881 gaagtacacg agcagtgggc cgacctcgtc gtttgcgggg cggtagtggc gggtggggat
    11941 cggaccggcc gggccgggaa tcgaaagatc gctgacggcg ccgaccggca ggcgtttgcg
    12001 gtcgaccatc tgcgtgaact ggtgcacgtt ccgccggcag gtctccgggt cgtcggcggc
    12061 cgacaggcct tcctcgccgg acagcttctg gccggccaga atcagctgca tcgtcgggtc
    12121 cagcgtgttg ccgtcgatcg tgatcgaccg ggtcatgaac cgcttggcgg cgtcgggaac
    12181 cttcggcatc acaggggcca ccagccggtt ggctgtggtg acggcggcga ccctggctcg
    12241 gtccagccgc ggatgccggt gcaggtgccg cggcggcacc tcgggcctgt tcgacgtcga
    12301 acctgggaca ggggctgtgg cgctgtcagt catgtctggg gacggtatcg cgtccaggtg
    12361 ccgctggcag acttgaggcc atgaatgctc cttcgatcac tctcaatgac ggcaacgcca
    12421 ttcccggcgt gggtcttggt gtgttcaaga cacccccaaa ggagacggaa caggccgtca
    12481 gggctgcact cgacgccggc taccggcaca tcgacaccgc gtcggcatac cgcaacgagc
    12541 gcgaggtggg ccgcgcgatt gcgaaatcac ccactccgcg cgacgaaatc ttcgtcgtca
    12601 ccaagctgtg gaacgccgac caaggttacg acgccacgat gaaggcgttc gagaagagca
    12661 tgaagcggct gggcctcgac cgtgatggcg actacctcga cctctatctg atccactggc
    12721 cggtgcctgc gatcagcgaa ttcgtcgaca cctggcgggc gttcgccgca ctgcacgatc
    12781 tcgggcggat ccgctcgatc ggcgtctcga acttcgcacc ggagcatctc aacacgctga
    12841 tcgacgcgac gggcgtcgtc cccgtggtca accagatcga gctgcatcca ctgatgaccc
    12901 agggtgagct gcgggagatg cacgcgcagc tgggtatcgc gaccgaggcg tggagtccac
    12961 tgggtcgggg ccggctgctg gaccacccga cgatctcctc catcgccgac gcgtatggga
    13021 agaccccagc gcaggccatt attcgctggc atttgcagat cggtaacatc gtcatcccca
    13081 agtcggtgaa ccctgagcgg attgcgagca acttcgacgt gttcgacttc gagttgagtg
    13141 acgacgacgt cgcgtcgatc tcctcgctcg acaacggcac ccggctgggc ccggacccac
    13201 gcaccttcaa cttcacaggc aggtgagatg acgccgaccg tcacgctcaa cgacgacaac
    13261 gtcattccgg tcctcggtct cggcgtcgct gaactctcgg aagaagacac cgaacgtgcg
    13321 gtcagcgcgg cgctagaggc cggataccgg ctgattgaca ccgcgtcggt gtacggcaac
    13381 gaggccgcgg tgggccgcgc gatcgccgcg tccgggctcc cccgcgacga gctgttcgtc
    13441 accaccaagg tcgcgaatgc cgaccagggt ttcaactcca ccaaggaggc gcttgccgcc
    13501 agcctggagc ggctcggcct ggaatacgtc gacatgtaca tgatccattg gcccgcagca
    13561 caattgggca aatacatcga cacctacggg tccatgcaga agatccagca ggaagggcag
    13621 atccggtcga tcggggtgtg caacttcctc ggggagcacc tgagcaccat ctccgacctg
    13681 acgtacacca acccggcggt caaccagatc gagctgcacc ccctgctgaa ccaggccgag
    13741 ctacgcgatt tgcacgccga tcgcggcatc gtcaccgagg cctacagccc gctgggtgta
    13801 ggcaacgtgc tggagaatcc gacgctcgtg tcgatcgccc aggcgcacgg caagtccccg
    13861 gcgcaggtgg cgatccgctg gagcatccag ctgggcaatg tggtgattcc gcggtcgggc
    13921 aaccccgagc ggatcaagga aaacatcgac gtgttcggct tcgagctgac cgacgacgag
    13981 atgtcgcaga tcaatgggct cgacaacggc acccggttcc gcgaggaccc ggcgacctac
    14041 acgggcacgt agtcgcttcc gcgcgctgac tctgcggtga gggcgcgagt tactcgaact
    14101 ttcgcgccct gagcgcagtg tcaattccct cagcccgtcg ggcaggtagc cgcagcgtcg
    14161 cgcagcctgt ccgccgacgc ctgatcaaca ggcagcccgt acccgcgcag caccgccgcg
    14221 aactgcaccg cgtactggca ccagaacgcc tcgttcggcg gcatccatgt cgacgggccc
    14281 tggtcgccct tgtcctgatt cgccggaccg tcaaccgcaa gcaggttcgc cgggtcgttg
    14341 gcgaaccgct cacgtaactc gtcggtccag ccgcgtgcac cgaggtccca tgccagggcc
    14401 agcggcacga tgtggtcgat ctgcaccgac gcgccggtct gttccccgcg caagaaagcg
    14461 atcatcgcgt tggtgtacgg gtcgtgcagc gtgcccgtcg caaccgcccg tgggcagcga
    14521 ctgatcgatg tgtaggtctt gtcgacgagg tcgcggtcga ggatgtcgtt tcgggtgtcg
    14581 cagccgttgt gcccgcccgg tgcgtcggtg tcgtcggtcc aggagtcgcc gaacgcggcg
    14641 cggcggtagt cgtagccgcg gatacggagg ggtatgaccg ggattccgct tagcacgtcg
    14701 acgcccggag cgacggtggg cacgtcggcc tgcgcgacga acgcgtcgga ccgatgccgc
    14761 cacgccgacg tgccggtctg gtaggccacc agcagcgtga ccacaaccgc ggccacgagc
    14821 cacatccagg ttcggcgcct catgacttgt cgaggtattc cacgcgatcc gtctcggtga
    14881 actgggctgc cagcactgcc attccgggct gattggggtt tcgctcgtag gccgtctcac
    14941 agaactcccg ggcggcttcg atgacctcct gatgctccga cagcgacaga aacctcaacg
    15001 tgatgggccg accagattga ctgtggccca gcacgtctcc ttcgcgacgc tcctgcaaat
    15061 cgaggtcggc gagcttgaac ccgtccaatg tgccggcgac ggctttcagc cgctgcccgg
    15121 cattggatcc ctcgggcagc cgggtggcga gcagacagag gctgggatgt tggccgcgtc
    15181 cgatccggcc gcgcagctga tggagctggc tgatcccgaa gcggtcggca tccatcacga
    15241 acatcaccgt cgcgttcggg acatcgacgc cgacctcgat gacggtggtg cacaccagta
    15301 catcgatctg gccgccgcgg aaggcggcca tcaccgcgtc cttctcgtca gccgacagcc
    15361 ggccgtgcat gagacccacc cggagcctcg agagcggccc gcgtcgcagg cggtcgtaga
    15421 gctcgaggac tgtggtggcc ggcggaccgc ccgcgtcgga gaccggattg tcggtctcgt
    15481 cgatgcggga cgccaccacg taggcctgac gtccggcggc gacctcttcg gtaatccgtt
    15541 gccacgcgcg gtcgagccac tgcggcttgt cctggacgaa gatcgtgttg gtggtgatgg
    15601 gttggcggcc gcggggcagc tcccgcagtg tcgacgtctc caggtcgccg tacacggtga
    15661 gggcgacggt gcgcggaatg ggggtagcgg tcatcaccag gagatgcggc gtcaaatcat
    15721 caggagcctt ggcgcgcaac cgatctcgtt gctccacgcc gaatcggtgc tgctcgtcga
    15781 caacgaccat gccgagttgc ttgaactcca ccgcgtcttg cagcagcgcg tgggttccga
    15841 tgacgatgcc ggcttcccca gaggctacct cgtcgcgcac gtggcgtttc tgttgcgctg
    15901 acatcgatcc ggtgagcagt gccaccgcgg tcgcaccctc cgcgcccccg agttggccgg
    15961 ccatggcgag cgggccgagg atgtcgcgga cggaccgggc atgttgggcg gcaagcactt
    16021 ccgtcggcgc caacagcgca cactggtagc cggcatcgac catctgcagc atcgccagta
    16081 ccgacacgat cgttttgccg gacccgacct caccctgcaa catgcgattc atgggcctgg
    16141 tacggtccag ttcggttgac agcacgtcga gtacgtcgcg ctgtccatcc gtcagttcga
    16201 acggcaactg ccgcagtagc gcggcgacca atccgtcgtc gcgacgtggc gcgggcggac
    16261 cggattcgcc cagttcgccg tatcgccgcg ccaccagcgc ccattgcagc ccgaccgcct
    16321 cgtcgaacgt gagacggttt tgggctcggt cgcgctccgt cgcgttctcg gccaggtgaa
    16381 tggcgcgcag tgcctcgtct tcactgatca gattccgttg ccgcagaagc gattccggca
    16441 gcgggtcctc gatggggtcc agcatggcca ggacttggcg cacacacgcg tagatgtccc
    16501 agctctgcac cttggcgctg gccggataga tcggaaagaa ctcccgctcg aacaccgcga
    16561 gcagctcgtc ctctccggct gcccccgatg ccgtagcgat ggtcttcagc gacttcgtcc
    16621 ccggcttgtc tccgctgggc gagtggagca cgaggaacgc gggatgggtc aattgcattg
    16681 tgcgaccgaa gtacttgacc tcgccggaca gcatgatgcg ggtatcgggg acgagtttgc
    16741 cgatcatcca gtcggcgttg aagaacgtgg cggtcacctt cggccgatgg ttgccgagcg
    16801 tcacgcgcag ccacttgcgt ttgcgcttgt cgggccgcgg tttcatgtag tcggatttgg
    16861 tgtcggtgat gacgtcgacg aacgtgacgt gctcgccttc ttccacgtcg aggtcctcgt
    16921 ctccgccgcg caccgtcatg ccctcgctgt acttgcgggg atagtggcgc agtaggtcgt
    16981 tgacggtgcg gatcccgaag ttctcctcga gctttttcgc tgtcgtcctg ccgagcacga
    17041 agtcgagccg gtcgccgagc gtggccaccg ctactcgact ccgatcagca acgcgtcacc
    17101 gcgatggccg gtcttgtagg tcaccaactc ggtgcccgga tgatgacggt gcacgtgcgc
    17161 ctgcaggtcc tcgcccaccc agtcgtcgac gcccgcgccg acgagcaccg tgaccagctc
    17221 accaccggca cccagcagca ggtcgagcag ccgacgtgcc gtcgcggcga tgtcgtggcg
    17281 caccaccagg acctcgtcgc cagcgatccc cagcccgtca cccggctcgc acgggccggc
    17341 gaaggtgagc gcctcttcgt cggcaacccg cacggaagcg tgccgtgtgg ctccggccgc
    17401 ccgcgtcatc gagtacccgt catcgacgac ggcccggccg ggttcgtgca cggcgagcgc
    17461 ggccagcccc tgcaccatcg atcctgtggg taccggcacg acgctgagtc cccagccggt
    17521 ggccgccgcg cagcccgcca cgagttcctc ggcggccacg aagccgttgg gcagcaccac
    17581 gacctggccc gcgccggtgt cgacgaccgc gcgcagcagc tccttggcgc tgatcgacgc
    17641 ggacgggtcg ggtcggtcgt cggcgcaccg cagcacatgc gcaccttcgc tggtgaagag
    17701 ctgctccgcg ccgtccccgt cgaccacggc cagcaccacg cgccggcgcg tccatcggct
    17761 gtgcatcacg gcgccgtgca gcgtcgagat ctggatctgg ctgggtgtgc cgacggcgag
    17821 accggcctcg atcgcggccc cggcgtcgtc ggcgtgcacg tgcaccgagt agcggtcgcc
    17881 accgtcgccg gcgatggcca ccgagtcgcc gagctcctcg agccgttcgc gcaaggcgtc
    17941 gagccgctgg ccggttgcgc tgatctgata catcacctcg tagtgaggcg ggcggacgtc
    18001 agcgctcggt tccgtggcgt cttggacggg gggcgacgga acgtactcgc cgcgtgcggg
    18061 ggtgtggccg gtgagggtgg ccgccagcgc gtcgagcaac accaccagcc cccggccgcc
    18121 agcgtcgacg accccgaact cggccagcac gccgagctgc tccggcgtgc gttcgagcgc
    18181 ggtgacggcc gcctccgcgg cggaaagcac ggtgctggcc aggtcgtcgc cgtcgtcggc
    18241 gcaatgctct gcccgggcgg cggccgcctg cagcaccgac acgatcgtgc ccgccacctc
    18301 ctcgcccatc gaaccgaccg ccaaggccac cccgtgccgc aacgcggcgc cgaacagcac
    18361 cgcgtcgacg tcggcgagat ggctgtccgt gtcggccgag gcagacgccg tcacgtcagc
    18421 gagaccgcgc aggatctgcg acagaatcac cccggagttg ccgcgcgcgc cgcgcagtgc
    18481 gccgcgggcc agggctgccg ccacctgcga cacgtcgccg gatctcttgg cgtgccgctc
    18541 ggcctcggca agagcggccc gcatcgtgaa cagcatgttg gtgccggtat cggcgtccgc
    18601 gacggggaag acgttgagcc ggttgatctc ctcggcgtgg accaccaggc cgccgaccgc
    18661 ggcgtgcgcc cagtcgagca ggccggacgc gtccaaccgc cgacccgaca tcacacctcc
    18721 tcgcccggcg cacccgctgc gagcctatcg agtggcagtg acaggccttg gcgactcccc
    18781 ctgccgccgt cattttggtg atcatccgca aggccggtat cctgattcgg ttgtcgggtc
    18841 acccgatctg gttgctggcc tgcgacgaca cactgactga ccgatccgaa tggagtttct
    18901 gctatggctg ccgtctgcga tatctgcggg aagggtccgg gcttcggcaa gaacgtgtcg
    18961 cactcgcacc ggcggactaa ccgtcgctgg aatcccaaca tccagaccgt gcgcgcggtc
    19021 gctcgccccg gcggcaacaa gcagcggctg aacgtgtgca cctcttgcct gaaggcgggc
    19081 aaggtcgcac gcggctgaat ttccgcgccg aaagtgaagc cagggctgtg aatccgccag
    19141 atcaacgacc aggaattcgg tttcggcgca aaggcagtgt caggacagcc gccagtcgac
    19201 cggctcggcg tccaggtccg acaacagtgc gttcgcgcgg ctgaacggcc gcgacccgaa
    19261 gaacccccgc tgcgccgaca gcggcgacgg gtgaacggag tcgatggtcg cgacccgcga
    19321 gccgccctcg gccagcatcg gcttcagcgt tgccgcgtcg cgtccccaca ggatggcaac
    19381 cagcggctgc ggacggtccg caagcgctcg gatggcacac tcggtgacca cttcccagcc
    19441 cttgccgcgg tgcgaagcag gattctgagg ccgcaccgtc agcaccctgt tcagcagcaa
    19501 caccccgcgc tcggcccacg gcgtcaggtc gccgttcgac ggcgtcgggt agccgagatc
    19561 ggtgcagtac tccgcgaaga tgttgtccag gctgcgcggt aatggcctga cgtcgggggc
    19621 gacggagaag ctcagcccga cggcgtgtcc cggcgtcgga tacgggtctt gcccgacgac
    19681 gagtacccgg accttctcaa atggaaaggt gaacgcgcgc aatacatttc gtcctgcggg
    19741 caggtagcgc tggcctgccg caagctcggc gcgcagaaat tcccccatcg ccgcgacctg
    19801 atccgcgacg ggttccagcg cctgcgccca accgtcgtcg acgagttcct tgagcggacg
    19861 tgtcgtcacg gccgacaacc tacgccagcc cgttcaccaa cagcaccacc gtgtccgccc
    19921 cggcgtgacg gtcttcggag atcgaccggt actccggcga ttcggcccag gcccggaacg
    19981 cggcctcgtc gggaaacgac accaacacca ccttgtcgag gttccactca ccctcgatga
    20041 cctgcggcga ctcgtcggcg gccagcaacg tcccggggtg tcggcggaag acctccatga
    20101 atctcgcctg atagcggtca taggcggccc ggtcggtgaa cttcaactga gcgatcgcat
    20161 agacggtcac gccgtcacgc taactgccga acgactgcca gcccgcgtgt ccttgccatt
    20221 ccgcgctgtc gacgagcacc cggggcggcc cgtccaccac ggcgccgatc acccgccagc
    20281 cgtgcggcac ccgcccgtcg aacaccgcga ccagcgcgtg gtcctccccg ccgccgagca
    20341 cccatgccca ggcgtcggcg ccgaccacct gagcggcctc gagcaccgcg tcacgatcgc
    20401 cggccagccc cgccgtcgac aactcgatgc cgacaccgga cgcctcggcg atgtgccgca
    20461 cgtcggcgac caggccgtcc gagatgtccg tcatcgcgcg cgcccccgcc ttggccgcga
    20521 gcacaccctg accgtagggc ggctcgggca cgagatggcg gcgccgcaga tcgtcgaaac
    20581 caggaaaatt caactgccac aacgagtatc ccgcggctga tcggccaagc tccccgcaga
    20641 cagcgatcac cgatcccgcg gtcgcaccgc tgcgggtcac tggcgcgcgg ccggccaggt
    20701 cgccgagcac cgtgaccgac accacccatt gcgggctgct gaccagatcg ccgccgacga
    20761 tgccggaccc gacccggcga gcctcgtccc acattccgtc cgccagctcg ccgacctgcg
    20821 cgacgggcgt gtcagccggc gcgccgaacg acaccacgaa acccgttgcg gcagcgccca
    20881 ttgcctcgac gtcggccgcg ttctgggcca tcgctttgcg cccgatgtca tgcggcgtgg
    20941 accagtcccg gcgaaagtgc cgggattcga cgagcatgtc ggtggagacg acgacgcggc
    21001 catccggggc cgcgaccacc gccgcgtcgt cgccgggacc gagcagcgtg tgggcaccgt
    21061 ggcggcggcc ggcgagcaat cggtcgatca cggcgaactc gcccaggtcc gcgagcgtcg
    21121 gctccttgct cacgtctgac tcctgccctg aaggcccgtg ttacctgcgg tacttttgat
    21181 ctctgccggc ctgaagcacc ggccagtgta ttgcggagga gaggtgcggg tggtcgaggc
    21241 attcatgctg attcagaccg aggtaggccg cgccgaagtc gtcgccaagc aactcgccac
    21301 ccttgccggg gtgctgaccg ccgaatacgt caccggaccc tatgacgtca tcgtgcgcat
    21361 cggcgccgac acgctcgagc ggcttcaggc tgacgtcgtg cccagcgtgc agcagaccgc
    21421 gggcatcacc cgcacgctga cgtgcccgat cgccggcaag tcccagccct gagcgacggc
    21481 cccccgcggg cgttgctcgt cgccgcggtc ggcgtcgcgg tggcggccgc ggtcgccgtg
    21541 ctggtgatcg ccgtgacgac gtcgcgccag ccgcaggaac gacctctcgt cgtcccggcg
    21601 gtgcctgtcc cgcaggcgga cgcccccgag tgcgcgacat tgatgggcgc gctgccgcaa
    21661 cgcctcggcg actaccagcg ggccaccccc gccgacccgg ttccgccggg caccgccgcg
    21721 tggcggtcag gcagcgacga cgagccgatc ctgctgcgct gcggggtggg ccagcccacg
    21781 gatttcgtcg tcggcgcgca gatcaacgtc gtcaacaccg tgcagtggtt ccgcgtgccc
    21841 gaacagagca ccggccgttc cacctggttc gtcgtcgacc ggccggtcta cgtcgcgctg
    21901 accctgcctc agggaaccgg ggccacaccg atccaggaga tgtcggacgt cgtcgcggcg
    21961 aagctgcctg cggtgccgat caggcccgga ccgccgcgct agaactcagc gcaggccgac
    22021 gcccctggcc agcgcggtgt cgaccatcgc ggcgagcagg gtcgggtagt ccacaccgct
    22081 ggcggcccac atgcgcgggt acatcgagat cgtcgtgaaa ccgggcatgg tgttgatctc
    22141 gttcaccagc ggcccgttct ccgtgaggaa gaagtcgacg cgggccaggc cctggcagtc
    22201 gatcgcgtga aacgcccgaa tcgccaacgc ccgcacgtct tcggcgacct catcgtcgac
    22261 ctttgccggc acgtcgagtt cggccccctc gtcgagatat ttggtcgcga aatcgtagaa
    22321 cgcgtcctcg cggccacgca ctccttcgac gcgaatctca ccgacggtgc tcgcctgcac
    22381 ggtgccgtcg ggcagttcga gcacaccaca ctccagctcc cgcccgcgaa tcgccttttc
    22441 caccaacact tttggatcgt gctgacgcgc gttctcgatg gccgcgggca gttcttccca
    22501 tgacgacacc cggctcacgc cgatcgacga cccggccctg gcgggtttga cgaacacggg
    22561 caggccgagg cggtttttgt ctcgttcggt cagcgtggcc tgccgtgggc gtagcaccgt
    22621 gtggtcgccg atcggcagtc gctcggcggc cagcagcttc ttggtgaact ccttgtccat
    22681 gcccgcggcg ctggcgaaaa cacccgcgcc gacgtagggc accccggcca gctcgagcag
    22741 accctggatc gtgccgtcct caccataggg tccgtgtagc accgggaaaa ccacgtcgac
    22801 ggacgccagc gcctcaccga gcgcaagctc cgcacccgac gacgcggtga cctccggtaa
    22861 tcgaccacct tcgatcgcca ggttgccggg atcggcgttg gtcagcaccc aggtgccctc
    22921 ccgggtgatg ccgatcggca cggcatcgaa ccgcgccggg tcaaggtggc gcaggatgct
    22981 gccagccgtc acgcaggaga tcgcgtgctc gttgctgcgc cctccgtaga ccacggcgac
    23041 gcggaccctg ccggcagtca caagctagag aggctaccgg tcagaccgcc gacaacgcgc
    23101 tctcgacgtc ggcgaccagg tcatcggtgt cctcgatccc cgctgacagc cgcgcgaacc
    23161 cgtcgaccac ctggtcgccc cagcgggcgc ggccgtcgac ggacgtatgg atcccgccga
    23221 agcgctgcat ctgcgcggaa gccgtcgggt aggccgggtc gtcaggcaga cccggatacc
    23281 tcacggagcg caccgccgga tgactatgca gcatcgtggc gagtgcttgc gcgttgcggc
    23341 attggcgctc cagccgcaac cctgcggtgc ccagactgcg caggacgagc cacgcttcga
    23401 acgcaccgag aatcggcccg gtcaacagcc gctcggcggc gaccgcggct atcagctccg
    23461 gcttgctgcc ggcgacgtaa cccgcgacga gatcgctgtg cccggccaac gcttttgtcg
    23521 cgctggccac caccagatcg gctccgagcc ccagcggctg ctgacccagc ggcgtcgctg
    23581 cggtgttgtc gacgacgaga gtcgcccccc ggccgcgaca catcagcgcc agttggtgca
    23641 gttccactac atcgagctgc gggttcaccg gtgtctcggc cagcaccacg tcggcgtcag
    23701 ccgccgcttc gcacatctgg ccggcggtcg cctcgcgcac tgtaattcct tggcgtgcca
    23761 agtattcgac cgcatagcgg cggacctggt agtaaccgtc ggcaggcatc acaaggaccc
    23821 cgcgcggcct gacgagcgct cgcagcaccg acgtgatcgc cgccatcccc gagccgaaca
    23881 caagcgccgc cgtcgcaccc tcgagttggg cgatggccga ctcgagttgc cgccatgtgg
    23941 ggttcgagct ccggccgta
//
LOCUS       DASWBF010000231         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_222138,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000231 DASWBF010000000
VERSION     DASWBF010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222138"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1530)
                     /locus_tag="VF874_08280"
     CDS             complement(130..1530)
                     /locus_tag="VF874_08280"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX9176079.1"
                     /translation="MQAITDWSKNLRVEVRGDDVAQHAGCVLPRMLADATGLTEGLSA
                     AVSRPEVTHDRGGVLRDVAVSICDGGRDISDVVVLGQQPRLFGPVASIPTVWRSLNEL
                     DASDIAAITVVRNRIRPKVWEAIAARHGAIPPAPTCDGDLGATIVIRIDASLVDSHSD
                     KQHAAGNFKGGWGFHPLMAWCDNTGELLAIIPRPGNAGSNTAADHIAIIDAAIAAVPA
                     KWRHNLLVTIDGAGSSHAVVEHLTKLNARPGWSVHYSVGFDLDERVRVAIGQLPASTW
                     EPALDQHGTARQDAQVAELTGLLRESVGGDRLDGWPADMRILVRREKIDEGTQLSLFE
                     QHNGYRYQVLATATRGGQVQRLEARHRVHARVEGFIRNAKDTGLGRWPSSSFAINTAW
                     ITTAALAIDLLCWMRLLLLDGPLAKAEPATLRHRLLHTAVRLISHSRHLILRVCQTWP
                     WAQEFADAFNRALAIP"
BASE COUNT          255 a          593 c          614 g          292 t
ORIGIN      
        1 gtcgtagtct cacacgagac gatccgggcc tggtgccaag cacgccgcgt gtcgtgcggt
       61 gacgccgggt tccatgggcc cggtggtggt ttgctccttc ctggggtcgt tggggcagtg
      121 gcctgttggt caggggatgg ccagggcgcg gttgaaggcg tcggcgaatt cttgtgccca
      181 gggccaggtt tggcagacac ggaggatcag gtggcgggag tggctgatca ggcggacggc
      241 ggtgtgcagc agccggtggc gcagcgtggc gggctcggcc ttggccagcg gcccgtccag
      301 caggagcagc ctcatccagc acagcaggtc gatggcgagg gcggcggtgg tgatccaggc
      361 ggtgttgatg gcgaacgagg acgagggcca gcggcccagc ccggtgtcct tggcgttgcg
      421 gatgaatccc tccacgcggg cgtgcacccg gtggcgggcc tcaagccgct ggacctgccc
      481 gccgcgagtg gcggtggcga gcacttggta gcggtagccg ttgtgctgct cgaacaacga
      541 cagctgggtg ccttcgtcga tcttttcccg ccgcaccagg atccgcatgt cagcgggcca
      601 gccatcgagc cggtccccgc cgacggactc acgcagcagc ccggtcagct ccgccacctg
      661 cgcgtcctgg cgcgccgtgc cgtgctggtc gagcgccggc tcccaggtgc tggcgggcag
      721 ctgcccgatc gcgacccgca cccgctcgtc gaggtcgaac ccgaccgagt agtgcaccga
      781 ccagcccggc cgggcattga gcttcgtcag gtgctcgacc acggcgtggc tgctacccgc
      841 gccgtcgatg gtgaccagca ggttgtgtcg ccacttggcg gggaccgcgg caatcgcggc
      901 gtcgatgatc gcgatgtggt cggcggcggt gttggagccg gcgttgcccg gccgggggat
      961 gatcgccagc agctcgccgg tgttgtcgca ccaggccatc agcggatgaa acccccagcc
     1021 gcccttgaag ttgcccgccg cgtgctgttt gtcgctgtgc gagtcgacca gcgacgcgtc
     1081 gatgcggatc acgatggtgg cgccgaggtc gccgtcgcag gtgggcgcgg gcgggatcgc
     1141 accatgccgg gccgcgatcg cctcccacac cttcggccgg atccggttgc gcaccacggt
     1201 gatcgcggcg atgtcggacg cgtcgagctc gttcaacgac cgccacaccg tggggatcga
     1261 cgccaccggc ccgaacagcc gcggctgctg gcccagcacg acgacatcgg agatgtcgcg
     1321 gccgccgtcg cagatcgata ccgccacgtc ccgcagcacc ccgccccggt cgtgggtcac
     1381 ctccggccgc gacaccgctg ccgacaaccc ctcggtgagc ccggtcgcat ccgccagcat
     1441 ccggggcagc acgcacccag cgtgctgcgc cacgtcgtcg ccgcggacct ctacccgcag
     1501 attcttcgac caatcggtga tagcttgcat ctgttaggtg cactccctcg cgtgataatc
     1561 gaacgtcggc aacgtcgatt ctcgttgagg cagtgcacct ttcacattca cgacacgcat
     1621 ccgctgtcaa ctacgtgaaa tgtcgaggtc agatggccag aggctttctg ttcctccgag
     1681 tgtcgatcct tgggagcagc agccccgccc gcacccatgt ccatgccggt caccgtgatg
     1741 cggcgcagga ccat
//
LOCUS       DASWBF010000232        32942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_21056, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000232 DASWBF010000000
VERSION     DASWBF010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 32942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32942
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21056"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF874_08285"
     CDS             complement(<1..372)
                     /locus_tag="VF874_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEX9176080.1"
                     /translation="MPARSAAGRGAGRRGLRSALTTVVRSITVAAGVFAPGHLGELTR
                     QVPFELVDSVLVQTRATERRLRALPSRVGVYFVLALCLFPGVGYPKVWDKLIAALDTG
                     SVPSVSGKALRDLRRRVGVAPL"
     gene            complement(524..1231)
                     /locus_tag="VF874_08290"
                     /pseudo
     CDS             complement(524..1231)
                     /locus_tag="VF874_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
     gene            1273..2208
                     /gene="coaA"
                     /locus_tag="VF874_08295"
     CDS             1273..2208
                     /gene="coaA"
                     /locus_tag="VF874_08295"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEX9176081.1"
                     /translation="MARPSEPSPYVEFDRAQWRELRKSTPLPLTEEELYRLRGLGEQI
                     DLAEVAEVYLPLARLIHLQVAARQRLFDATAEFLGEQHQPERPVPFVIGVAGSVAVGK
                     STTARVLQALLARWEHHPRVDLVTTDGFLYPNAELSRRNLMHRKGFPESYDRRALLRF
                     VTAVKSGADKVCAPVYSHLLYDIVPGEMQAVRHPDILILEGLNVLQTGPTLMVSDLFD
                     FSIYVDARVKDIEQWYILRFLTMRSTAFANPASHFHHYAALNDQKAVAAAREIWRSIN
                     RPNLIENILPTRPRATLVLRKDADHSINRLRLRKL"
     gene            complement(2276..2686)
                     /locus_tag="VF874_08300"
     CDS             complement(2276..2686)
                     /locus_tag="VF874_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176082.1"
                     /translation="MTAITTRPLSASTDALLRFALRADATLTGMAGLAVAALANPLST
                     IGGMSPTTEYLIGAAFVTYGVVVYYLAALPSLRRVGIGVSLANVVCTLATLAIVLTDA
                     APLTEAGVAVTLATGVYTAFFAYVQYLGVRRLKA"
     gene            complement(2805..3638)
                     /locus_tag="VF874_08305"
     CDS             complement(2805..3638)
                     /locus_tag="VF874_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9176083.1"
                     /translation="MATTPASGSTPRPPEGDWLGTPFLTLRRDGPFAVLTIDRPEARN
                     ALTPAMYFGIRYAVGRVNEDKSLAGLLITGTGDVFAPGGDMGGGGADDWITFGSALSM
                     DITPFDMLRTSVKPVVSAVNGLCQGGGLQIALCSDMSVVSERATFRVPELFRGYADTY
                     YSQMLARLIGPVRTRDLMFTGRVLSAQEALAWGMVARVVAHDDLLETARDVLAQCCRT
                     APGARAVIKSSLDAYMGLYDRIGMTTSLYSAEAVEGVRAFKGRRSPDWVHPELRGSGR
                     L"
     gene            complement(3656..6643)
                     /locus_tag="VF874_08310"
     CDS             complement(3656..6643)
                     /locus_tag="VF874_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9176084.1"
                     /translation="MDSDPYSTQRDVGSAIVSQLRAAGFDDADEIGRGGFGVVYRCTQ
                     VGLDRTVAIKVLTTDVAEDRERFLREQRAMGRLTGHPNIAAVLQVGETDGGFPYLVMQ
                     YHGQGSLEDRIRRLGLLPVDEVLHIGVKMAGALESAHRLGIMHRDVKPANILLTDYGE
                     PVLSDFGIAHVVGGFKTAAGTFTGSPAFTAPEILGGDPPSVASDIYGLGATLFCALTG
                     HAAYERKSGEKVLTQFLRIATEPVPDLRDSDVPADIADAIATMMSREPESRPTAVAAG
                     EELQKLQSRRGAPVDDMALPTDEAQERRDEPRPRRRAGGGLPLELTSFVGRGTELAEL
                     KSLLSTSRLVTLTGIGGVGKTRLAIRAASEVVPDYPDGVRLVELGELRDGSLLIDVVA
                     AALGMRDESGRPLRDVLVDFLRSRRLLLVLDNCEQVVDDVARLVEEVLSACPDLRLLT
                     TTRERLGVAGESVLRIAPLAYPGADDTLGGTPGYDAIALFAERATAAVPGFRLTEENK
                     RTVADIVSRLEGLPLAIELAAARLRAMSLDQLLERLADRYQLLTRGARGVPTRQRTLS
                     WSVGWSYDLCTPAEQELWRRLSVFAGSFELEAAQDICGADMTEYELLDVVASLVDKSI
                     LVRTESDGVVRFRLLDTLREYGRQRIEQTAEFSEIRRRHTDWYRRLAQQAAEQWFSSR
                     QLDWLARVDRETPNIREALEFSLSQDGQAALAISIALQPIWMCQGMLREAWRWTNRAL
                     VRTPRDATLERVRALYSVALLSPLLCDLATGAASAAEARALVEQLDAGRTTAGVALAD
                     GITALVNGDFERASVLLEEGLSGGSDDSEQENAMLLLGWTLEFAGDTARGLIWHEKAL
                     ALAKSHDEVVYRAYALWSLGVGWWQYGRPERAEQLLQDGVRATRKVNDRRGAAGCLEA
                     LAWIAASRDPTRAAVMMGAADTLARAVGTQPIPLPRLVVFHEECVRSTRDALGVEAFD
                     VARQRGSAMTFDDAAAYALSETA"
     gene            complement(6680..7711)
                     /locus_tag="VF874_08315"
     CDS             complement(6680..7711)
                     /locus_tag="VF874_08315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9176085.1"
                     /translation="MTAASTPRLKIDRGIPSALDRIPEDASALERQGYDGCWSAEINH
                     DPFLPLALAAEHTSRVELGTSIAVAFARNPMTIANVGWDLQAYSQGRFILGLGSQIQA
                     HIEKRFSMPWSRPVHRMREFILALRAIWSCWQDGTTLRFEGDFYTHKLMTPMFVPEPT
                     PYGFPKVFIAAVGEVMTEMCGEVADGMIGHAFTTKRYLDEVTIPTLLRGIERSGRDRG
                     DFELMCPVFVVTGESDAELASAAAATRKQIAFYASTPAYRKVLELHGWGDLHGELHRL
                     SLQGDWDTMGSLIDDEILDAFAVVAPVDTLAGKLRARCDGVIDRVLLGFPKTVSEATV
                     AEILHEVRR"
     gene            7823..9315
                     /locus_tag="VF874_08320"
                     /pseudo
     CDS             7823..9315
                     /locus_tag="VF874_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975813.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
     gene            complement(9305..10402)
                     /locus_tag="VF874_08325"
     CDS             complement(9305..10402)
                     /locus_tag="VF874_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9176086.1"
                     /translation="MNVGSIVKEFGGVLPRALTGLDTADWSPASPRGARQLVEVMLDQ
                     LVLSGMSLIGGRATPVMLPASTYTAAAQELSALGIQGAYAEPSPLRVRAMRRHRIGHI
                     SYEKLKFDHHPLLPNYLIAQGLSGTATAGAYLIRHVDGPRPWVVWVHGAGQGQPVDLF
                     FSRAGRLHHELGFNVALPVQPGHGFRRTAWPTYPDREPLANVAGMIRAVSEVRALVRW
                     LQPQSTSIAVSGVSLGSPVAALVSALEQHVDAVAVYTPILGLNAMIAHHLARGGPSSE
                     RFRPLLQSDDVTAMTSVIDPLRVEPAAPPDRRLVVGAWNDQMALREPAIALHQRWGGQ
                     LYWYDGSHVGHIFSRRIQAVTEEFLAGALTA"
     gene            10530..11921
                     /locus_tag="VF874_08330"
     CDS             10530..11921
                     /locus_tag="VF874_08330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9176087.1"
                     /translation="MNRLSSADAAFWFAESPSWHMHVGASAVCDPSDAPDFSFESVRK
                     LIATRLPELPQLRWRVAGAPLGLDRPWFVEDHDLDIDFHVRHIAVPSPGGRREFDELV
                     GRLMSYKLDRSKPLWELWFIEGLENGRAAIVTKMHHAVIDGVSGAGLSEILLDVTPEP
                     RPPAVEVNRSLVGVQLPRPEIRALGGLVNVGVKTPYRIARLVEQTVRQQLATRGIANK
                     PPRYFDAPTTRFNAPISPHRRISGTSVPLDRVKAVKGAYGVKLNDVVLALVAGAMRRY
                     LEDRGELPEKSLVSQLPVSTRPEGNTEIGNQISTMTVSLATDVVDPAERIRAIYANSQ
                     GAKEMARALTANQIMGLSETTPPGLLSLAARAYTASGIGRTLAPINVVISNVPGPDFP
                     LYLCGAVLERLMPVGPLVLDVGLNITCFSYRGSVDFGFVTTPEIARDIEDMADAIEPP
                     LRELEVAAGLVTS"
     gene            12109..13839
                     /locus_tag="VF874_08335"
     CDS             12109..13839
                     /locus_tag="VF874_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX9176088.1"
                     /translation="MDKRGDRREYSSAYLRRTYREGQRVRNETVANLSALPEHVVDWI
                     DAGLKGEQLVPAGSEFSIARSLPHGHVAAVAAMARTLGLPALLGPSCRARDLVLALIV
                     SRVVRPASKLATAAWWPDVTLGVDLDVAGASTDEIYAAMDWLLERQDTIEAKLAAKHL
                     GPDVNPSRMALFDLTSSWVTGRCCELAARGYSRDGKKGCEQIEYGILTDPAGRPVAVR
                     VFEGNTADPDAFTDIVEVVRDIFGLDNLVLVGDRGMITSARIEALRELNDDPDTATDF
                     GWITALRAPQIATLAADDGPLQMSLFDTQDLAEITHPDFPGERLIACRNPLLAAERTR
                     KRDELLAATEHLLEPIIARVAAGRLAGADKIGVAVGKVIDKHKVGKHFHYTITDTSLA
                     IERRHDQIAAEAALDGIYVLRTSVPADQLDAAGVVTGYKNLANIERDFRIIKVDDLDL
                     RPIHHRLDDRVRAHVLICMLACYLIWHLRKAWAPMTFTDEHPPQRDNPVAPAQRSAGA
                     DAKASTQHDSQGNPLRSFRGLLAHLATLTRNEIRYHHTDIEIPMLTDPTDDQRRAFDL
                     IGTPIPLTAA"
     gene            complement(13918..14850)
                     /locus_tag="VF874_08340"
     CDS             complement(13918..14850)
                     /locus_tag="VF874_08340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9176089.1"
                     /translation="MTAMRISLIAGLNNDAPVDSCIRDLTQARDEGFGRVWMTQMPYD
                     ADLLTVLAVALREVPDVEVASGVVPIQNQHPMLLAQRALTLNMIGNGRFTLGIGMTHR
                     TVTEGMWGISWDRSVRRLNEYLDGLLPLLAKEAVDATGEIVTTRGALQMPNAPTPEVY
                     VAALGPQMLRVAGRRTGGTVTWMTGPKTLKEHVGPALRTAATEAGRPDGAVQVAAALP
                     TSVTEDVDAARSLAAQQFAMYGYLPSYRAMLDREGYAGPEDAALIGSEETVWARLDEV
                     RDAGVDEFIGIPFDPSPEGRERSRNCLRAWESRV"
     gene            complement(14847..15230)
                     /locus_tag="VF874_08345"
     CDS             complement(14847..15230)
                     /locus_tag="VF874_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9176090.1"
                     /translation="MFRPTRTAREVAELYTLSVWNERNFELADEIIADTMIHHGTGQA
                     HVLTREQVRQTIEQTCANFDKLRFNINLLIAGDDGEHVAVVYDSRITTKDGAEIESAA
                     LEVLRVVDGQITEVWYSPSQQGAWL"
     gene            complement(15300..16367)
                     /locus_tag="VF874_08350"
     CDS             complement(15300..16367)
                     /locus_tag="VF874_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03857 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX9176091.1"
                     /translation="MTELPAQPAAKVLDELGYYLLAGAGGEGPATLMDEARRGEEMGF
                     GTAFISERWNVKEASSLVGAACAVTQRMQIATAATNHNTRHPLITGSWATTMHQLSGG
                     RFTLGIGRGIAAIYGAFGIPAVTTAQMEDFAHVMRRLWHGEIIFNHDGPIGKYPILFL
                     DPDFNEDIRLALVAFGPDTLALGGRAFDDVVLHTYFTPETLQRCVKTVKDAAEQAGRD
                     PESVRVWSCFATVGDHLPEELRLKKTVARLATYLQGYGDLLVKTNNWDPAELQRFRED
                     PVVTSIAGGIDHKATAEQIEHIATLIPDEWLEPSATGSAQQCVDRVRKEFDYGADCVI
                     MHGATPDELAPIVGVYCETVG"
     gene            complement(16364..16741)
                     /locus_tag="VF874_08355"
     CDS             complement(16364..16741)
                     /locus_tag="VF874_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9176092.1"
                     /translation="MSPRTAREVVELYNLVVWNERNFDLADELMGDTVIRHDVGEAQT
                     LTHEQAVQRIVDLWEFVEKLHFDLNIVIAADDGEHVAIVYDSTMTNKDATQMPIGSME
                     IFRVVDGRITEVWNCGYKQGVWR"
     gene            complement(16738..17796)
                     /locus_tag="VF874_08360"
     CDS             complement(16738..17796)
                     /locus_tag="VF874_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9176093.1"
                     /translation="MTSGTAEVPASIGDVTPQWLTEVLGTDVTDIHAEQIAQDSGFSS
                     LLYRLHITGSAGVPSTVIVKLPAQSEARGAMEMLGGYRRELSYYRDIAGRAPMDTPHV
                     YAALMAQNSVDFVLVLEDLRNWENADHLAGLSMDRARLVIAQLAGLHAWSVDPANAVA
                     VQAFPSVDTAINRDILHPAFGPGWQIYCEKSDAPVPPAVARFAERFAEQAPTALQALT
                     EREMLLHGDIRADNMFFDGDRLKVVDFQFTARGCGAADVAYLVSQGLPTQARRGHDEA
                     LVREYLACLAARGVDDYSFEDAWRHYRFGVAYLMVLPVITLLGWDAMPERSRQLCVTL
                     TERAVAAIDDIGALEVFG"
     gene            17866..18513
                     /locus_tag="VF874_08365"
     CDS             17866..18513
                     /locus_tag="VF874_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9176094.1"
                     /translation="MPSPARGLTQPQRVEMSSRRLVEAAAELIAEKGWDATTAAEIGR
                     RAGYSRAMVHTRYGSKDAILEAFFQHEYVQRLTPAPEPDATGLDEALAPFDRIQEVYA
                     EAPDFLRAMFAMTFEGVKSTSPVRPILRTWLMRGVDAVDAGLRKGIRDGSVRADIDLD
                     RAANDVSAGVFGVAFQWMLSDRYDLARELSYLRARLVSEYGAPRRKGRRNAARHS"
     gene            18494..19345
                     /locus_tag="VF874_08370"
     CDS             18494..19345
                     /locus_tag="VF874_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176095.1"
                     /translation="MPRDIRELTGEPEAYAAELRRRWGGLLSYRYIGRTYAQMDLGPA
                     DNTVTLRRDMRNGTDGLLLAVLGIASPEGGHMSDLEAVPNPVIHSCQVLDAGRDVHRI
                     EIVSEELKRGRQMGYSRSKIVDADQPTRVLALTEGQGISIGTPPEGLQKMSVEPIEVT
                     DSPELPPLWQVFGGHRRADGRWVLPELDDKVASPDAALHIGPQFVMLETAALDLAADI
                     AGTDQLQGVSSHTMFVARGKVGPFRVAGEPFAGADGTVAVRVVMHDEGADDRVTTVGS
                     YLFRVEV"
     gene            19349..19798
                     /locus_tag="VF874_08375"
     CDS             19349..19798
                     /locus_tag="VF874_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9176096.1"
                     /translation="METWELVACERIRDTLARYNWAGDSLRLDEFAETFCEDGELQLR
                     GDEPVRGRTAIVAFIGGVATSDAPASGPPRIVRHNLTNIRFLEVTPQHARVASYFTVF
                     TDIGLDHYGRYRDTLVPVGEKWLIRHRFVSTDWAAPNSTMAEPQIRS"
     gene            19837..20244
                     /locus_tag="VF874_08380"
     CDS             19837..20244
                     /locus_tag="VF874_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX9176097.1"
                     /translation="MTAIRVREAAELLGVSDDSVRRWIDDGSLPADLDRSGRKVIAGD
                     VLAEFARAHATTPPKDPLSVGSSARNRFAGLVTKVVADGVMAQVEMQCGPFTVVSLMS
                     SDAARELNLKPGSVAVAVVKATTVIVETPKGQS"
     gene            20241..20999
                     /gene="modA"
                     /locus_tag="VF874_08385"
     CDS             20241..20999
                     /gene="modA"
                     /locus_tag="VF874_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9176098.1"
                     /translation="MKHCWRLTLAIVLALAIGGCGSDQPSKSPEKIVVFAAASLNKSF
                     TEIGERFKTDNPGASVEFSFAGSSDLVTQLTQGASADVFASADSNNMQKAAQGGLLAG
                     DPVNFASNTLTIAVAPGNPKNISSFRDLTKPGLNVVICAPQVPCGSATEKVEQNTGVQ
                     LAPVSEESSVTDVLNKVLTGQADAGVVYVTDAIGAGDKVTAVPFPESANAVNVYPIAV
                     LKQSKNPELARKFVDLVTSEAGQKILNAAGFAKS"
     gene            complement(20971..21837)
                     /locus_tag="VF874_08390"
     CDS             complement(20971..21837)
                     /locus_tag="VF874_08390"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9176099.1"
                     /translation="MSANGPEGAVCRLRDPAPLFETNVEGLRHVLDAAVEANLKRFVF
                     TSTIGTIALSDDGPATEDLPFNWYEFGGGYIQSRVQAENLVVQHACERGLPAVVMCVA
                     NTYGPRDWQPTPHGSLVALVAAGRMPLHFQGLAYEVVGIEDAAEALVLAADNGRNGER
                     YIVSERFMSWRDIFDTAADAVGARRPRISVPFRLTSALGRLADVATRVLRRDLPINSV
                     SVRLAHIMSPLDHGKATRELGWQPSSTPDSIRKAAQFYGALRKRRFAGKVRVADCKTS
                     LSVITIWRTQPR"
     gene            21883..22875
                     /locus_tag="VF874_08395"
     CDS             21883..22875
                     /locus_tag="VF874_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX9176100.1"
                     /translation="MKVPFTWKVTGWFMIGWSAEFPAGEVRPLHYFGEDLVAYRDESG
                     ELHVLQAHCRHLGAHIGHGGKVVGDCVECPFHGWRWGPDGTNRYIPYQPDRPNRALTL
                     RVYPVMEQYGCVFAWHQPQGKEPQWDMPDIFTSFPQFETDPSAYYRPYPEFSRRTENE
                     AVHPQIVAENGPDSSHFKYVHKATVTPVSLDWRIVDQEWRFLTGWPDVRADDPDKMAL
                     RIHSHMFGLGGAISAFEGSSNHRLIFAVTPVDDGSSTMFYSIWWPKLDGETSDVPPEA
                     VRKQVEKQFLRTVWEDLDIWRYQEYIERPSLPKVDAKPYMALRKWATQFYETPA"
     gene            22872..23492
                     /locus_tag="VF874_08400"
     CDS             22872..23492
                     /locus_tag="VF874_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX9176101.1"
                     /translation="MTVDLPELIAPGHTAIVTQECQGAVMGPNAGLAVFAKEARREAL
                     PNIVRLLPAARAAGVQVVHCLVQRRPDGLGSNHNAKIFAVGRTEVDITPGSEGATLLP
                     ELGPEPTDVVLHRWHGVGPMGGTDLDANLRNLGVSTIVAVGVSLNVAIPNLVMDAVNG
                     AYRVVLPRDAVAGFPADYGNAVIDNSLSLLATITTTDDLLRAWTTS"
     gene            23477..25129
                     /locus_tag="VF874_08405"
     CDS             23477..25129
                     /locus_tag="VF874_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9176102.1"
                     /translation="MDDIVSFTVPAVAQAVATAIGDRVLVTQGDKQYTYAQIVERSTR
                     LASYLHSRGLGCHTERSSIGGHEVGQDLVGIYAHNGNEYVEAMLGAWRARVAPFNINY
                     RYVKAELQHLLADAGATALIYHAAFAPRVAEVLPDLPNLKVLIQIADDSGNELLDGAV
                     DYETVIASSSSEPPPVQPSPDDLYVLYTGGTTGMPKGVLWRQHDIFVTSFGGRNLVTG
                     EVMGSYDEIVARAAENPGTKIMILPPLMHGAAQWGVMTAITTGQSLVFASVVDRLDAA
                     EVVRTIERERVSVVTVVGDAIARPLLAAIEKGTADVSSLVAIANGGALLTPSIKERLI
                     KTLPNGMVIDGVGSSETGAQMHHMSISGAVSTGRFNAGPDTFVAAEDLSSILPPGHQG
                     IGWLAQRGYVPLGYKGDADKTAATFPVIDGMRYAVPGDRARHLEDGSIELLGRDSVTI
                     NSGGEKIFAEEVEMAIASHPAVADVVVAGRPSERWGQEVVAVVALADGASVDASELIE
                     HAAQSIARYKLPKAVVFRPAIERSPAGKADYRWAREQAVSDA"
     gene            complement(25358..26503)
                     /locus_tag="VF874_08410"
     CDS             complement(25358..26503)
                     /locus_tag="VF874_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9176103.1"
                     /translation="MNDVAIIGVGLHPFGRFEGKSAMQMGADAIFAAAADAGVRWKDI
                     QFGVGGSWTVANPDAIVSLVGLTGIPFTDVFNACATAASAAKACADGIRLGDYDIGIA
                     VGMDKHPRGAFTEDPALVGMPSWYAQNGQYLTTKFFGMKANRYLHEHGISEQTLAKVA
                     AKNFRNGALNPNAFRRKPIPEDQILNSPMLNYPLTQYMFCAPDEGAAAVVMCRGDIAH
                     RYTSKPVYLRAAEVRTRRYGAYEVNTTFAPVDEDVSPTVYAARAAFDKAGVAPGDVDV
                     IQLQDTDAGAEVIHMAECGFCVDGDQEKLLADGATEIGGAMPVNTDGGLIANGEPIGA
                     SGLRQIHELVRQLRGEVGERQVPGEPRVGFAQLYGAPGTAAATVLST"
     gene            26620..27831
                     /locus_tag="VF874_08415"
     CDS             26620..27831
                     /locus_tag="VF874_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9176104.1"
                     /translation="METQAQEILSADRAAEWKRDPYPFFAERRRQAGVFRGPVIDYSR
                     LPESMRPKHVFSALSFDAVNTVFRDGRVFDSSGYDTSIGLFTGRTILAMEGKEHREHR
                     NLVSTAFKSKSLARWEPEIVRPVCNALIDEFAESGEADLVRKFTFEFPTRVISRLLGL
                     PEDDLPMFRRRAIQLISYNYDYKQAFEASAALKEYFLEQIEQRKSKPTEDIIGDLVTA
                     EIEGEKLSDEAIYSFLRLLLPAGLETTYRSSGNLLFNLLTRPDQFAAVQADRDLIGPA
                     IEENLRYETPLTSVQRFATEDTELEGVEIPADSMLDVCIGSANRDEHRWERSEEFDIF
                     RKPIPHISFAVGEHTCMGLHLARLETRVALECLLDRLTNIALITDDDPHIRGNPFRSP
                     TALPVTFDVVG"
     gene            complement(27839..28687)
                     /locus_tag="VF874_08420"
     CDS             complement(27839..28687)
                     /locus_tag="VF874_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEX9176105.1"
                     /translation="MGKLDNKVAVISGAARGQGRSHAVSLAAEGAGIIALDICADLDG
                     STYPLARPEDLEETARLVEKEGVPVHTAIVDVHERTPVWKAIEEGVNELGHLDVVVAN
                     AGIAAMGKGQTIQSWSDTIDTDLVGVFNVIQGSLPHLNDGASIIATGSLAALIGNATN
                     QGPGSSAYSLAKQVVARYVNDLSIQLANRMIRVNAVHPANVNTDMLQNEGMYQQFRPD
                     KESPTREDAEAVFPVMQAMPVPYVEPQDVSNAVVFLASDDARFITGSQLRVDAGGYVK
                     MVPWKG"
     gene            complement(28687..30240)
                     /locus_tag="VF874_08425"
     CDS             complement(28687..30240)
                     /locus_tag="VF874_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9176106.1"
                     /translation="MSDVAQDQAAPSIEVGRRVAERAESRMLIDGDLTASVSGAEFDN
                     VSPATGLVLGRTAAAEVADMDRVIAAARRAFDDTDWSTNRELRKRWLLQLQSALEAEK
                     EDLRTELIAEVGCPVMTTHSAQVDWPLAESLRYPARLIDDFEWERTLDGGGLFGERNV
                     RTVVKEPVGVVAAITPSNFPIEVILNKLGPALATGNTVVLKPDPHTPWNATRLGRLIA
                     EHTDIPPGVVNVVSTPSNDVAAILATDPRVDMISFTGSTAVGKMLVRQGADTMKHTFL
                     ELGGKSALIVLDDADPAKVVPSAVGVCVHAGQACALTTRMLIHESLFDEAVANVTAVF
                     QMVPVGDPALPDTLVGPVISATQKQRVLSACEQARRDGAEITTGGGSFDDLPAHLAGG
                     HYVQPTVIVGADNSAAIAQEEIFGPVLVIIPFADDDEAVRLANDTLYGLAGAVLSAST
                     ERGMSIARRIRTGSIGVNGGMFYGADAPFGGYKSSGIGRQCGIEGFQQYLETKTIGCR
                     MPRTRERQQ"
     gene            30312..31526
                     /locus_tag="VF874_08430"
     CDS             30312..31526
                     /locus_tag="VF874_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9176107.1"
                     /translation="MEGFRVLEIAQFTFVPAAGAILADWGADVIKVEHPVRGDTQRGF
                     LNMGGHELNPDRHPLIEHPNRGKRSVGIDVSRPDGQEVLYELAKTADVFLTNYLPAAR
                     QKNKFDVEHIRAANSKIIYARGSAYGDKGPERSVGGYDGTAFWTRSGVGHSLTPPELG
                     GMLSQGIPAFGDSIGGMNIAGGISAALLHRERTGEALEVDVSLLSTAWWAAGASVTHG
                     MDTGETMRAEMPHSGGSAVNPFLGNYETSDGGTINLCIVSPTGYIRDTFEHLGIPEAA
                     DDPRFSDVLPLMENAEAASELIVAAIGSKPFEYWRQHLKTMKGQWAPFQSFIDLATDE
                     QAIVNDMVVEVEPADGGKPFKVVRGPVQFNHEPLVTTRAPQASEHTELVLMELGMDWD
                     RIAALKDSGAIA"
     gene            complement(31551..>32942)
                     /locus_tag="VF874_08435"
     CDS             complement(31551..>32942)
                     /locus_tag="VF874_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX9176108.1"
                     /translation="AFPPEPTASMTGIEPDVVRTLARDLAHTPRAAVYGRFGTCVGRS
                     GTLTAYLIDAVNLVAGKLDRPGGSVFSTLGMPGQKLGTMAMGASLRRAYRRKRTRVGG
                     FGAVIGSEPAALMAKEITTTGTRQIKALFVSAGNPVLSVPNGNELEEALETLQLMVGL
                     DLYVTETTAHCDYILPVTTMYERDDFAVTFQTFQATPFRQATEAVVPPRGQARSEWEI
                     IDELMKRTAIRAPVVAGLSAGRRLLGLFGRRLHPRPLIDMMIRMSEGGDRFGLRRGGL
                     SFSRLISEHPHGAVVAPHIRTGVLGEVVVYRRGRVRLAHEDIAEEIAKLSERSEPADY
                     PMRMIGMREPRSENSWMHNAPLLMRSNRFHRALMHRDDAMARRIADGDTVAVSSPYGR
                     ISLPVCLTADIVPGTIAVPHGWGHKGTGGWQLANRARGANVNQLTPSDPDDLESLAGM
                     SWLTGVPVEVQRA"
BASE COUNT         5730 a        11130 c        10584 g         5498 t
ORIGIN      
        1 cagcggcgcg acaccgacgc ggcgacgcag atcccgtaac gccttgcccg acaccgacgg
       61 caccgatccg gtgtcgaggg cggcgatcag cttgtcccac accttcggat agcccactcc
      121 ggggaaaagg cacagtgcca gaacgaaata cacacccacc cgcgacggca gcgcccgcaa
      181 ccgccgctcg gtggctcggg tctgcaccag caccgaatcg accaactcga acggcacctg
      241 ccgggtcagc tcgcccaggt gaccgggagc gaacacgccc gccgccaccg tgatcgaccg
      301 gaccacggtg gtcaatgccg aacgcagtcc ccggcgaccg gcgccgcgcc cggccgcgga
      361 ccgggcgggc atgacagaat ccagcacagc ggctcctcgg tagagcgtga tcttggttcg
      421 atccctcttc taccaggggc cgcgcccact tcaccgcaac tgcgcaacag tcacgcgtgt
      481 cacacttgac tcaacagcca cacccttaac tacccggcat tgagactagc ccggcctcct
      541 tgcggcagtg ctcggtgtgg tggccgggat aagactcatg cgcgacgagc cgcggcaggt
      601 tggacatctg ctgcttgagg tcggcgttga ccgcgacgcg tgaccggtat ccgcccgagt
      661 agtagttgaa cccggaccac ggcttgtcag tgacgacctc gtactcgacg acctcgccgt
      721 cgggaagcgg aaacgacgcg cggacccggt cgcgcagcgc gctggagaac gcgtcgatgc
      781 attcgcgcag ccgttccggc gggatttcgt cgctcaaccg gtaggcctgc atgcggtcgg
      841 caagcggacc ggtaccgccg agcgcttcat ccaggtgagc atgcgcagcg cgataccgct
      901 ccgggtcccc cttcgagatc gacacgtcga agtaggcgcg cacctcgtcg acgaacccga
      961 cctgctcccc ggcgaacttg cggcccgcgc attccagagc gcgcaggtgg gcggcgacga
     1021 agtcggcgcg tgccggctcc aggcccgccg ggatcaggct cagtaactcg gcggcgcggc
     1081 gggccaactg cgacggactc ggcgcaggct cgttctcgac gatccggcgc agcgccgggt
     1141 cgccggtgaa cgcgtcgacg taaccggact cgatccggtc gaatcgcagc ccgagcagaa
     1201 ggtattcgcg cacgagagcg tcggagtcca ccccgccaat ctagaggccg caagtcctgc
     1261 aaggatggcc tgatggcgcg gccgagcgag cccagcccgt acgtcgagtt cgaccgggcg
     1321 caatggcgcg aactacgtaa gtcgacgccg ctgccgctga ccgaggaaga gctgtatcgg
     1381 ctgcggggcc tcggtgagca gatcgacttg gccgaggtcg ccgaggtcta cctgccgctc
     1441 gcgcggttga tccacctaca ggtcgccgcc cggcagcggc tgttcgacgc gaccgcagag
     1501 ttcctgggcg agcagcacca gccggagcgg ccggtgccgt tcgtgatcgg cgtcgcgggc
     1561 agcgtggcag tcggcaagtc caccaccgct cgcgtgcttc aggcgttgtt ggctcggtgg
     1621 gaacaccacc cgcgagtcga cctggtcacc accgacggct tcctttatcc caatgccgag
     1681 ctcagtcggc gaaacctaat gcaccgcaag ggttttccgg agagttatga ccgccgggcc
     1741 ctgctgcggt tcgtcaccgc ggtgaaatcg ggcgccgaca aagtgtgcgc cccggtgtac
     1801 tcgcatctgc tctacgacat cgtgccgggc gagatgcagg ccgtccgtca ccctgacatc
     1861 ttgattctcg agggactcaa cgtgttacag accgggccga cgctgatggt gtcggacctg
     1921 ttcgacttct cgatctacgt cgacgcccgc gtcaaagaca tcgagcagtg gtacatcttg
     1981 cggttcttga ccatgcgctc gaccgcgttc gcgaaccccg cgtcgcactt ccaccactat
     2041 gccgcgctga acgaccagaa ggccgtcgcg gcggctcgcg agatctggcg gtcgatcaac
     2101 cggccgaacc tgatcgaaaa catcctgccc acccggccgc gcgccacgct ggtgctgcgc
     2161 aaggatgccg accactccat caaccggcta cggctgcgca agctgtagaa aggccggtgc
     2221 ggggcggctc gctgacgtac caccggcggt gccccgcacc cggcatatgc gggagtcacg
     2281 ccttcagacg acgcaccccg aggtattgca cgtaggcgaa gaacgcggtg tagacgccag
     2341 tggcgagggt gacggcgacc ccggcctcgg tcagcggcgc ggcgtcggtc agcacgatcg
     2401 caagcgtcgc cagcgtgcac accacgttgg ccaggctgac gccgatcccg acgcgccgca
     2461 gcgacggcag cgcggccagg tagtacacca ctacgccgta ggtcacgaac gcggcgccga
     2521 tcaggtactc ggtggtcggc gacatcccgc cgatcgtcga cagcgggttg gccaacgccg
     2581 cgacggccag gccggccatc ccggtgagcg tcgcgtcggc gcgcaacgcg aagcgcaaca
     2641 gcgcgtcggt ggaggcggac aacgggcggg tggtgatggc ggtcatcggg tgctcctttg
     2701 cgacttaagt tgacgtcgtc gaacaccttc gatgctgctg accagccact gccaccacca
     2761 cgccgcgcgc tgccaaacac tgccaatcgc ccggcggccg gggctcagag acgcccgctg
     2821 ccccgcagct cggggtgcac ccagtcaggt gagcgacgtc ctttgaacgc ccgcacccct
     2881 tccaccgcct cggccgagta caggctcgtg gtcatcccga tgcggtcgta caaccccatg
     2941 tacgcatcca ggctggattt gatcactgca cgggcgcccg gcgcggttcg gcaacactgc
     3001 gcgagcacat cccgggccgt ctccaacaga tcgtcgtgcg cgaccacccg ggcgaccatg
     3061 ccccaggcca acgcctcctg cgcgctcagc acccggccgg tgaacatcag atcgcgggtc
     3121 cggaccggcc cgatgagccg cgccagcatc tggctgtagt aggtgtcggc gtagccgcgg
     3181 aacagctccg gcacccggaa cgttgcgcgc tcgctgacca ccgacatgtc gctgcacagt
     3241 gcgatctgca ggccaccgcc ctggcacagc ccgttgaccg ctgacaccac cggcttgacg
     3301 gaggtccgca gcatgtcgaa cggcgtgatg tccatcgaca gcgccgagcc gaacgtgatc
     3361 cagtcgtcgg ctccgccgcc gcccatgtcg ccgcccggtg cgaagacgtc gccggtgccg
     3421 gtgatcaaca acccggccag gctcttgtct tcgttgaccc gccccaccgc gtaccggatg
     3481 ccgaagtaca tcgccggggt cagcgcgttg cgcgcctcgg gacggtcgat ggtcagaacc
     3541 gcgaacggcc cgtcgcgccg caacgtgaga aacggtgttc ccagccagtc accttcgggt
     3601 ggccggggcg tgctgccgga cgccggtgtc gtggccatcg gtctcctcgt cgtcgctatg
     3661 cggtttcgga cagtgcgtag gcagctgcat cgtcgaaagt cattgcgctg ccacgctggc
     3721 gcgccacgtc gaacgcctcg accccgagtg catcccgagt gctgcgcaca cattcttcgt
     3781 gaaagacgac gagccgggga agcggaatcg gttgggtgcc caccgcccga gccagcgtgt
     3841 ccgccgcacc catcatcacg gcggcgcgcg tcgggtcgcg agacgccgca atccatgcca
     3901 gcgcctccaa gcacccggcc gcgccgcgtc tgtcgttgac cttgcgggtc gccctcacgc
     3961 cgtcctgcaa cagctgttca gcgcgctcag gccggccgta ctgccaccag ccgacgccca
     4021 acgaccacag cgcgtaggcc cgatacacca cttcgtcatg ggatttcgcc agggcaagcg
     4081 ccttttcgtg ccagatcaac ccgcgggcgg tatccccggc aaactcgagt gtccagccca
     4141 gcaggagcat ggcgttctcc tgctcgctgt cgtcgcttcc cccgctcagt ccctcctcga
     4201 ggagcacgga tgcgcgctcg aagtcgccgt tgacaagcgc agtgatgccg tcggccagcg
     4261 ccactccggc cgttgtcctt ccagcgtcca attgctcgac cagggctcgc gcctcagccg
     4321 cgctcgccgc cccagtcgcc aggtcacaca gcagcggcga aagcaaagcg acactgtaga
     4381 gcgcccgcac gcgctcaaga gtcgcgtctc gcggtgttcg caccaacgcg cggttcgtcc
     4441 agcgccacgc ttcccgcagc atcccctggc acatccaaat cggttgcagt gcaatgctga
     4501 tcgcgagggc agcctggccg tcttgcgaca ggctgaactc gagcgcctcc cggatgttgg
     4561 gcgtctcacg atcgacacgg gcgagccagt cgagttggcg cgaactgaac cactgctcgg
     4621 ccgcctgttg agcaagccgc cgataccagt ccgtgtgtct acggcgaatc tccgagaact
     4681 cagccgtctg ctcgatccgc tgccggccgt attcgcgcag agtatcgagc aggcgaaacc
     4741 gaaccacccc gtccgattcg gtccgcacca gaatcgactt gtccaccagc gacgccacca
     4801 cgtcgagaag ctcgtattcg gtcatgtcgg cgccgcaaat gtcttgtgcc gcttcgagtt
     4861 cgaaactgcc ggcgaacacc gacaaccgtc tccacagctc ctgctcggcg ggtgtgcaca
     4921 ggtcgtagct ccagccgaca ctccaactca gtgtccgctg tcgggtcggg acgccgcggg
     4981 caccgcgggt gagaagttgg taccggtcgg ccaaccgctc cagcagctgg tcgagcgaca
     5041 tcgcgcgcag ccgtgcggcc gcgagctcga tcgccagcgg aagtccttcc agacgggaga
     5101 cgatgtcggc cacggtgcgc ttgttctctt ccgtgagccg aaagcccggg accgccgccg
     5161 tggcccgctc cgcgaacaac gcgatggcgt cgtagccggg cgtcccgcca agagtgtcgt
     5221 cggcaccggg atacgcgagc ggggcgatcc gcagcaccga ttcgccggcc acgccgagcc
     5281 gctcccgggt ggtggtgagg agccgcagat ccgggcaggc gctgagcact tcctcgacaa
     5341 gccgcgccac gtcgtcgacc acctgttcgc agttgtcgag caccaacagc agccggcgtg
     5401 accgcaggaa gtcgacgagc acgtcgcgca acggccgccc cgactcgtcg cgcatgccca
     5461 acgccgcggc caccacgtcg atcagcaacg agccgtcgcg cagctccccc agctcgacga
     5521 gccggacacc gtcggggtaa tccggcacca cctcggaggc ggcccggatc gccaggcggg
     5581 tcttgcctac tcccccgatg ccggtcaacg tcaccaggcg cgatgtcgac agcagcgact
     5641 tcagctccgc cagctcggtg ccgcgaccta cgaaactggt cagttcgagc ggaagcccgc
     5701 cgccagcacg gcgcctcgga cgcggctcat cccggcgctc ctgggcttcg tccgtgggca
     5761 gcgccatgtc gtcgacgggc gccccgcggc gagactgaag tttctgcagt tcctctccgg
     5821 cggcgacggc agtcggcctg ctctcgggtt cgcgcgacat catcgtcgcg atggcatcgg
     5881 cgatgtccgc gggcacgtcg ctatcgcgca gatcgggcac gggttcggtg gcgatgcgca
     5941 ggaattgcgt cagcaccttt tcaccgctct tgcgttcgta ggccgcatgg ccggtcagcg
     6001 cgcagaacag tgtggcaccg aggccgtaga tgtccgatgc gaccgacggc ggatcgccgc
     6061 cgaggatctc cggggcggtg aacgccggag atccggtgaa agtgcctgcg gcggttttga
     6121 atccgccgac gacgtgcgcg atcccgaagt cgctcaacac cggctcgccg tagtcggtga
     6181 gaaggatgtt cgccggcttg acgtcgcggt gcataatccc cagccgatgc gccgactcca
     6241 gcgcaccggc catcttcacc ccgatgtgca acacctcgtc gacgggcagc agacccagtc
     6301 gtcgaattcg atcttcgagc gagccttgcc cgtggtactg catcaccagg tacgggaagc
     6361 cgccgtcggt ctccccaacc tgcaacaccg cggcgatgtt gggatgtccc gtcagtcggc
     6421 ccatcgcgcg ctgttcccgc aggaaccgct cgcggtcctc ggcgacgtcg gtggtgagga
     6481 ctttgatcgc cacggtgcgg tccaacccga cctgcgtaca ccggtacacg accccgaaac
     6541 cgccgcggcc gatctcgtcg gcgtcgtcga acccggccgc acgcaactga gagacgatcg
     6601 ctgagccgac gtcgcgctgc gtgctgtacg ggtcgctatc catcaccggc tttctgctcg
     6661 ctctgagcat aggcgtcgat tatctgcgca cctcatggag gatctcggca accgtggcct
     6721 cggagacggt cttcggaaag cccaggagga cgcgatcgat cacgccgtcg cagcgagccc
     6781 ggagcttgcc ggccaacgtg tcgaccggtg cgacaacggc gaaggcgtcg aggatttcgt
     6841 cgtcgatgag cgatcccatc gtgtcccaat ccccttgaag ggacagccgg tgcagctcgc
     6901 cgtgaaggtc accccagccg tgcaactcca gcactttgcg gtacgcgggc gtcgacgcgt
     6961 agaacgcgat ctgcttgcgc gtcgcggccg cggcggacgc cagttcggca tcggactccc
     7021 cggtcacgac gaaaaccggg cacatcagct cgaagtcacc ccgatcgcga cccgagcgtt
     7081 cgatcccccg cagcaacgtc ggtatcgtca cctcgtcgag gtagcgcttc gtcgtgaacg
     7141 catggccgat catgccgtcg gcgacttcgc cgcacatctc ggtcatcacc tcgcccacgg
     7201 ccgcgatgaa caccttcggg aacccgtacg gcgtgggctc gggcacgaac atcggcgtca
     7261 tcaatttgtg ggtgtagaag tcaccttcga agcgcagcgt cgtgccatcc tgccagcacg
     7321 accagatcgc gcgcagcgca aggatgaact cgcgcatgcg gtgcaccgga cggctccacg
     7381 gcatgctgaa tcgcttctcg atgtgagcct ggatctgcga tccgaggccg aggatgaacc
     7441 ggccctgcga gtaggcctgc agatcccagc cgacgttggc gatcgtcatc gggttgcgcg
     7501 cgaacgctac cgcgatgctg gtgcccagtt cgacgcgcga cgtgtgctcg gcggccagcg
     7561 ccagcggcag aaacggatcg tggttgatct cggccgacca gcacccgtcg tagccttggc
     7621 gttccagagc cgacgcgtcc tcggggatgc ggtcgagtgc gctggggatg ccccggtcga
     7681 tcttgaggcg cggcgtcgac gcggcggtca tgcgagatac agtaagccat tcagtatggt
     7741 caccatgcgc cgagcgtgaa gccagggtcg tgaaaatgag caatcaacag cccggaattc
     7801 gatttcggcg tcgaggaggg gcatgacgaa caccgagggc tgggaggaca cgctcgacga
     7861 cctcaaccgt cgccgcgagc atgcctgggg catgggaggc ccggagcggc tggccaagca
     7921 ccgcgagaag ggcaagctcg acgcccgcgc ccggctcgat catcttctcg acaagggctc
     7981 gttcgccgag ttcggcacac tcgtaggcgg cgaggccgcc gccgacggaa tcgtcgccgg
     8041 atcgggctgc atcaacggcc ggccggtgat ggtaggcgcg gaggacttca ccacactggc
     8101 aggcacgatc gcgccgggca gcaattcgaa acgctaccgg cttgccgaat tggcgctacg
     8161 aaacaaaatc ccgctgatca tgctgctcga aggcgccggt ttccgtcccg gtggccatta
     8221 cggacgcgcg cccaccgatc tgatcgctca agcgaagtgc tcaggccgag tgccgacagt
     8281 ggcagcggtg ctcgggccgt cggccggcca cggcgccctg gtcgcgcggt gtgcgacttc
     8341 acgatcatga gcagccacgg cgcgatcttc accgcgggcc ctcccgtggt gaaagagtcc
     8401 acgggagagg acatttcgaa ggaggatctc ggcggtcccg atgtcgcggt gcccagtggg
     8461 gtgatccaca acgctgcaga cgacgacgag gccgtgctcg acgacatccg ccggtaccta
     8521 tcctatttcc cgtccagcgc atggtcgtac ccgtcggcgc tgccgcccga cgacaccacc
     8581 gaaccgcgcg ccaccccgga attgctcgac atcgtctccc gcgagggccg ccgcatctac
     8641 gacatgcggg ccgtgctcga cgtggtcttc gaccagccgg actggttgga ggtgcagccg
     8701 acgttcgggc gggcgatcat ctgcgcgctg gcccacctcg gtggtgagcc cgtcgcggtg
     8761 gtcgccaacc agccgaaggt gttggccggc tccatcgatg ccgacgccgc cgacaaggca
     8821 gcgcatttca tcaccgtcgc cgactcgttt cacctgccgc tcatttttct tgcggataac
     8881 cccggaatgc ttcccggcag ccagtccgag cgaagcggcg tgttgcgcag cggcgcaagg
     8941 atgttcgccg cccagacggc ggccaccacg ctgaagctgc acgtgaccct gcgcaaggct
     9001 tatggattcg gttcgatggt gatgtcgctg ctcgggttcg acgaccaggt cgccaccttc
     9061 gcgtatccgg gcgccacgat gggtgcgatg agcgccgcgg cgctgagccg cgcgtctcac
     9121 gccggacagg acgtctcggc caagctgcgc gagctggaac tgcaggcgtc cttccgatcc
     9181 gcggagcacc tgggtttcga cgagctcatc gatccccgcg agacgcgcaa cgcgctgctg
     9241 atcgcgcttc accgcggcgt ctacagtcgg caaaccgctg cggaaccggt gagccgcacc
     9301 gtgatcacgc cgtgagtgcg ccggcgagaa actcctcggt gaccgcctga atccggcggg
     9361 agaagatgtg cccgacgtgg ctgccgtcgt accagtaaag ctggccgccc caccgttggt
     9421 gaagcgcaat cgcaggctcg cgcaacgcca tctgatcgtt ccatgcgccc accaccagcc
     9481 gccggtccgg aggtgcggcg ggttcgacgc gcagagggtc gatcaccgac gtcatcgcgg
     9541 tcacgtcgtc tgactgaagc agcgggcgga aacgttcgct ggacggtccg ccgcgggcga
     9601 gatggtgcgc gatcatcgcg ttcagcccga ggatcggtgt gtacaccgcg acggcgtcga
     9661 catgctgctc cagcgcagac accagcgcgg cgaccgggct gcccaacgaa actcccgata
     9721 ccgcaatgct tgtcgattgg ggctggagcc atcgcacgag cgcacgcacc tccgagaccg
     9781 ctcgtatcat gcctgcgacg ttggccagcg gctcccggtc ggggtaggtg ggccacgcgg
     9841 tgcggcggaa tccgtgcccg ggctggacgg gcagagcgac gttgaaaccc agctcgtgat
     9901 gcaggcggcc ggcacgggag aagaaaagat cgaccggctg gccctgcccc gcgccatgta
     9961 cccagactac ccacggccgg ggaccgtcga cgtgccggat caggtaagcc cccgccgtcg
    10021 ctgtccccga aagaccctgg gctatcaggt aattcggcag cagcgggtgg tgatcgaact
    10081 tcagcttctc gtacgaaata tggccgatcc ggtggcggcg catggcgcga acccgcagcg
    10141 gggatggctc ggcgtacgcc ccttggatgc cgagcgccga caattcttgc gccgcagcag
    10201 tatacgtgct ggccggcagc atcaccgggg tggcacggcc accgatcagc gacatcccgc
    10261 tcagcaccag ctggtccagc atcacctcga ccagctggcg cgcacctcgc ggggacgcgg
    10321 gtgaccagtc ggcggtgtcc aagccggtca gggcgcgcgg caacacaccg ccgaactcct
    10381 tgacgatcga gcccacgttc atcccgaact ggcgaccccc tctcctgtcg cagccaccga
    10441 ataaattaca gtaatgcctc cggtacagat cgcccgcccc agtctgctat ttcggcccgc
    10501 acccggaaag accgccgtag ggtctcacca tgaaccggtt gtcaagcgcg gacgcagctt
    10561 tctggttcgc cgagtcccct tcctggcata tgcacgtcgg cgcctcagcc gtctgtgatc
    10621 cgtccgacgc gcccgatttc agtttcgagt cggtcaggaa gctgatcgcc acccgcttgc
    10681 ccgaactgcc gcagcttcgg tggcgagtcg ccggcgcacc actgggcctg gaccgaccgt
    10741 ggttcgtcga agaccatgat ctggacattg atttccacgt gcggcacatc gcagttccgt
    10801 cgccgggcgg ccgtcgtgaa ttcgacgaat tggtaggccg gttgatgtcc tacaagctcg
    10861 accggtcgaa gccgctgtgg gagctgtggt tcatcgaagg cctggagaac gggcgcgcgg
    10921 cgatcgtcac gaaaatgcac cacgccgtca tcgacggcgt gtccggcgcc gggctcagcg
    10981 agatcctgct ggacgtcacg cccgagccgc ggccaccggc ggtcgaagtc aaccggtcct
    11041 tggtcggcgt gcagcttccg cggccggaga tccgggcgct gggcggcctc gtcaacgtcg
    11101 gcgtcaagac cccgtaccga atcgcgcggc tcgtcgagca gacggtgcgc caacagcttg
    11161 ccacccgcgg catcgcgaac aagccgccac gttacttcga cgctccgaca acgcgtttca
    11221 acgccccgat ctcaccgcac cggcgcatca gcgggaccag tgtgccgctg gaccgggtga
    11281 aggcggtcaa gggcgcctac ggggtgaaac tcaacgacgt cgtactggcg ttggtggcgg
    11341 gggcaatgcg gcgctacctc gaagatcgtg gtgaattgcc ggagaagtcg ctcgtgtcgc
    11401 agctgccggt atccacccgt cccgagggca acacggagat cggcaaccag atcagcacca
    11461 tgacggtctc cttggccacc gatgtcgttg accctgccga gcggatcagg gccatctatg
    11521 cgaattcgca gggcgccaag gaaatggcca gggcgctgac cgcgaaccag atcatgggct
    11581 tgagcgagac gacgccgccc gggctgctgt cgttggccgc gcgtgcctac accgccagtg
    11641 gcatcggtcg aacgctagcc ccgatcaacg tggtgatttc caacgttccc ggacccgact
    11701 tcccgctgta tctgtgtggc gccgtgctcg agcggctgat gccggtgggt ccgctggttc
    11761 tcgacgtcgg cctgaacatc acctgctttt cctaccgcgg atccgtcgac ttcggtttcg
    11821 tgacgactcc ggagatcgcc cgtgacatcg aggacatggc tgacgcgatc gagccgccgt
    11881 tgcgcgaact ggaggttgcg gccggtctcg ttacctccta gaccgaagtt gcggtagctg
    11941 agcgggccgg ttgccttgct gacctgggga aagagttcgg gcaggccaga atatgtagtc
    12001 agaataaatg ggcgtgtcgg ctaggaacat ctcggtgtag ccgttaagct gcttgtcatg
    12061 tcgcggacaa gcgggaaggt ccacgtagtc agagttaaga agacccacgt ggacaagcgg
    12121 ggcgatcgcc gcgagtactc ctcggcgtat ctgcgccgca cctaccgcga gggccagcgg
    12181 gtgcgcaacg agacggtggc caacctgtct gcgctgcccg agcacgtcgt ggactggatc
    12241 gacgcggggc tcaagggcga gcagctggtg ccagccggca gcgagttcag catcgcccgg
    12301 tcgctgccgc acgggcacgt ggccgcggtc gcggcgatgg cgcgcacgct gggcttgccc
    12361 gccctgctgg ggccgtcgtg ccgggcacgg gatctggtgc tggcgctgat cgtctcgcgg
    12421 gtggtgcgcc cggcgtccaa gctggccacc gccgcgtggt ggcccgatgt caccctggga
    12481 gtcgatctcg atgtcgccgg cgccagcacc gacgagatct acgccgcgat ggactggctg
    12541 ctcgagcgcc aagacaccat cgaagccaag ctcgccgcca aacatcttgg gcccgacgtg
    12601 aatccgtcgc ggatggcgtt gttcgacctg accagctcgt gggtgaccgg gcgctgttgt
    12661 gagctggccg cgcgcggcta ctctcgcgac ggcaagaaag gttgtgagca gatcgaatac
    12721 gggatcctca ccgacccggc ggggcgcccg gtcgcggtgc gggtgttcga aggcaacacc
    12781 gccgacccgg acgcgttcac cgacatcgtc gaggtggtgc gcgatatctt cgggctcgac
    12841 aacctggtgc tggtgggcga tcgcggcatg atcacctccg cgcgcatcga agcgctgcgc
    12901 gaactcaacg acgatcccga caccgcaacc gatttcgggt ggatcaccgc gctgcgcgcc
    12961 ccgcagatcg ccaccctggc cgccgacgac gggccgctgc aaatgtcgtt gttcgacacc
    13021 caggacctcg ccgagatcac ccaccccgac ttccccggcg agcggctgat cgcctgccgc
    13081 aacccactgc tggccgccga acgcacccgc aaacgcgacg agctgctggc cgccaccgaa
    13141 cacctgctgg aaccgattat cgcccgcgtc gcggccggcc ggctggccgg cgccgacaag
    13201 atcggcgtcg cggtcggcaa ggtcatcgac aaacacaagg tgggcaagca cttccactac
    13261 accatcaccg acaccagcct ggccatcgaa cgccgccacg accagatcgc cgccgaagcc
    13321 gccctggacg gcatctacgt gctacgcacg tcggtgcccg ccgaccaact cgacgccgcc
    13381 ggggtggtca ccggctacaa aaacctggcc aacatcgaac gcgacttccg catcatcaaa
    13441 gtcgacgacc tcgacctgcg ccccatccac caccgcctcg acgaccgggt ccgcgcccac
    13501 gtgctgatct gcatgctggc ctgctacctc atctggcacc tgcgcaaagc ctgggcgcca
    13561 atgactttca ccgatgaaca cccaccacag cgggacaacc cggtcgcccc cgcgcaacgc
    13621 tcagcaggcg ccgacgccaa ggcctccacc cagcacgatt cccagggcaa cccgctgcga
    13681 agcttccgcg gcctgctcgc tcacctggcc accctcaccc gcaacgagat ccgctaccac
    13741 cacaccgaca tcgagatccc catgctcacc gaccccaccg acgatcaacg ccgcgccttc
    13801 gacctcatcg gcacacccat ccccctcacc gccgcgtagt cagacaccac cacaccacaa
    13861 accacgaacc cccagctcaa ccagggaaac cgccgtccaa caacccgtaa cttcggccta
    13921 gacgcgagat tcccacgccc gcaggcagtt tcgggaccgc tcgcggcctt ccggcgacgg
    13981 gtcgaacggg ataccgatga actcgtcgac gccggcgtcg cgcacttcgt caagcctggc
    14041 ccacaccgtc tcctcgctgc ctatcagcgc ggcgtcctct gggcccgcgt agccctcgcg
    14101 atcgagcatc gcccgatacg acggcagata tccgtacatc gcgaactgtt gcgcggcgag
    14161 cgagcgggcc gcgtcgacgt cctcggtgac gctggtcgga agcgcggcgg ccacctgcac
    14221 cgccccgtcc gggcgacctg cctcggtggc cgccgttcgc aaggcaggac cgacgtgctc
    14281 cttcagagtt ttcgggcccg tcatccacgt gaccgtgccg cccgtacgtc ggccggcgac
    14341 gcgcaacatc tgcggaccga gtgcggcgac gtacacctca ggcgtcggcg cgttcggcat
    14401 ctgcaacgct cccctggtgg tcacgatctc gccggttgcg tcgacggcct ccttcgcgag
    14461 cagcggcagc agcccgtcga ggtactcgtt gagccgcctc accgacctgt cccacgagat
    14521 gccccacatt ccttcggtca ccgtccgatg ggtcatgccg atgccgagcg tgaagcgtcc
    14581 gttgccaatc atgttgagtg tcaacgctcg ctgggcgagc agcatcggat gctggttctg
    14641 gatcggcacc acgccgctgg ccacctcgac gtcgggcact tcccgcagtg ccacagcgag
    14701 aacagtcagc aaatccgcgt cgtaaggcat ctgggtcatc cagactctcc cgaatccctc
    14761 gtcgcgggct tgagtcaagt cgcggatgca ggagtccacc ggtgcgtcgt tgttcaggcc
    14821 ggctatcagg ctgatgcgca tcgcggtcat aaccaagcgc cctgctggct gggcgagtac
    14881 cagacctcgg tgatctggcc gtcgaccacg cggagcacct ccaaggccgc ggactcgatc
    14941 tccgcgccat ccttcgtcgt gatcctcgag tcgtagacga ccgcgacgtg ttcgccgtcg
    15001 tcaccggcga tcaagaggtt gatgttgaag cgcagtttgt cgaaattcgc gcaggtctgt
    15061 tcaatggttt gacggacctg ctcgcgcgtc aacacatgtg cctgcccagt cccgtggtga
    15121 atcatggtgt ccgcgatgat ttcgtcggcg agctcgaagt tgcgctcgtt ccagaccgac
    15181 aaggtgtaca gctcggcaac ttcacgagcg gtgcgggtcg gtcggaacat gcgaacacct
    15241 ccagcgtggc gaatcgactc atggttcggc agaatcctcc cacgcgcgcc cgcgggaccc
    15301 taccccacgg tctcgcagta caccccgacg atcggggcga gctcgtcagg ggtggcgcca
    15361 tgcatgatca cgcagtcggc cccgtaatcg aactccttgc gcacgcgatc gacgcactgc
    15421 tgcgccgatc cggtggcaga cggctccagc cactcgtccg gaatcaacgt cgcgatgtgc
    15481 tcgatctgct cggcagtggc cttgtggtca atgcctccgg caatcgacgt gaccacgggg
    15541 tcttcccgaa atcgttgcag ctcagcagga tcccagttgt tggtcttcac caggagatcg
    15601 ccgtagccct gcaggtaggt ggccagccgg gcgacggtct tcttcagccg gagttcctcg
    15661 ggaaggtgat cacccacggt ggcaaagcat gaccatactc gcacgctctc cgggtcgcgg
    15721 ccagcctgct cggcggcgtc cttgaccgtc ttcacgcagc gctgcaacgt ttctggggtg
    15781 aagtaggtgt gcaggacgac gtcgtcgaac gcccggccgc cgagggccag cgtgtcgggc
    15841 ccgaacgcga cgagggccaa ccggatgtcc tcgttgaagt cgggatcgag aaacaagatc
    15901 gggtacttac cgatcggccc gtcgtggttg aagatgatct cgccgtgcca aagtcggcgc
    15961 atcacgtgtg cgaagtcttc catctgtgcg gtggtgacag ctggaatccc gaaggcgccg
    16021 tagatcgcgg cgatgccacg accgatgccg agcgtgaacc gtccaccgga gagttggtgc
    16081 atcgtggtgg cccaggatcc ggtgatcagc gggtggcggg tgttgtgatt ggttgcggcc
    16141 gtggcgatct gcatccgttg ggtgaccgcg caggccgcac ccaccagcga cgaggcctcc
    16201 ttgacgttcc agcgttcgga gatgaacgcg gtgccgaatc ccatctcctc tccgcggcgg
    16261 gcctcgtcca tcaacgtcgc gggaccctct ccgccggcgc cggccagcag gtagtagccc
    16321 agctcgtcaa gaaccttcgc cgccggctga gccggtaatt cagtcatcgc caaacccctt
    16381 gcttgtaccc gcaattccag acttcggtga tccggccgtc gacgacacgg aatatctcca
    16441 tgctgccaat cggcatctgg gtggcgtcct tattcgtcat tgtcgagtcg tagacgatcg
    16501 caacgtgctc gccatcgtcg gccgcaatga cgatattgag gtcgaagtgc agtttctcga
    16561 cgaactccca caggtcgacg atccgctgga ccgcctgctc gtgggtgagg gtctgcgcct
    16621 ccccgacgtc gtggcggatg accgtgtcgc ccatcagctc gtcggcaagg tcgaagttgc
    16681 gctcgttcca gacgaccagg ttgtacagct cgaccacctc gcgagcggtg cgtgggctca
    16741 tccgaacacc tccagtgcgc cgatgtcgtc gatggcggcc accgcccgct cggtgagcgt
    16801 cacgcacagc tgccgcgatc gctcgggcat cgcatcccac ccgagcagcg tgatcaccgg
    16861 aaggaccatc aggtacgcca cgccgaatcg gtagtgccgc caggcgtctt cgaacgagta
    16921 gtcgtcgacc ccgcgcgcgg ccagacacgc gaggtattcg cggaccagtg cctcgtcgtg
    16981 tccgcggcgc gcttgcgtgg ggaggccttg gctgaccaag taggcgacat cggccgcgcc
    17041 gcagccgcgg gcagtgaact ggaagtcgac caccttcagc cggtcgccgt cgaagaacat
    17101 gttgtcggcc ctgatgtcac cgtgcagcag catctcgcgc tcggtcagcg cctgcagggc
    17161 cgtcggcgcc tgctcggcga accgttcggc gaagcgcgcg acggcgggcg gaacgggcgc
    17221 gtcggacttt tcgcagtaga tctgccatcc cggcccgaac gcgggatgca ggatgtcacg
    17281 gttgatcgcc gtgtcaacgc tcggaaacgc ttgcactgca acagcgttcg cggggtcaac
    17341 cgaccacgcg tgcaaaccgg cgagttgtgc gatgacaagg cgggcccggt ccatcgacag
    17401 gccggccagg tggtcggcgt tctcccagtt ccgtaggtcc tccaggacca ggacgaagtc
    17461 gacggagttc tgtgccatca gcgcggcgta cacgtgcggc gtgtccatcg gcgcgcggcc
    17521 cgcgatgtcg cggtaatacg acagttcgcg gcggtagccg cccagcatct ccatggctcc
    17581 gcgagcctcc gactgcgccg gcagcttgac gatcacggtc gacggcacac ccgcactccc
    17641 ggtgatgtgg agccggtaca gcagcgacga gaacccgctg tcctgcgcga tctgctcggc
    17701 atggatgtcg gtgacgtcgg tacccagcac ctcggtcagc cactgtgggg tcacgtcgcc
    17761 gatgctggcc ggaacctccg ccgtgcctga agtcaccgat gactcctctg cgagatattt
    17821 gacgatagac aagtaacata attgtcgagc gtctaatatg caagggtgcc ttcgcccgca
    17881 cgaggactga cgcaaccgca gcgcgtcgag atgtcaagtc gcaggctggt cgaggcggcc
    17941 gccgagttga ttgccgaaaa aggctgggac gcaacgacag ccgcggagat cgggcgccgc
    18001 gctggctaca gcagggcgat ggtccacacc cggtacggaa gcaaggacgc gattctcgag
    18061 gcattcttcc agcacgagta tgtgcagcgg ctcaccccgg ctccggaacc cgacgcaacc
    18121 ggcctggacg aggcgttggc tccgttcgat cggatccagg aggtttacgc cgaggcgccc
    18181 gacttcttgc gcgcgatgtt cgcgatgacg ttcgaagggg tcaagtccac ctcgcccgtt
    18241 cgcccaatat tgcggacatg gctcatgcgt ggcgtcgatg cggtcgatgc gggcctgcga
    18301 aagggcatcc gcgacggctc ggttcgcgcc gacatcgacc tcgaccgagc tgccaacgac
    18361 gtcagcgccg gcgttttcgg ggtggcattt cagtggatgc tgtccgatcg ctacgacctc
    18421 gcacgcgaac tgagctacct tcgcgctcga ctggtgagcg agtacggcgc gccacgacgg
    18481 aagggacgac gcaatgccgc gcgacattcg tgagctgacc ggagagcccg aggcctacgc
    18541 cgccgagctt cgccggcgct ggggcgggct gctgagctac cgctacatcg gtcgcacgta
    18601 cgcgcagatg gacctcggtc ctgcggacaa caccgtcacg ctgcggcgcg acatgcgcaa
    18661 cggcacggac gggctgctgt tggccgtgct gggcatcgcg tcgccggagg gcggtcacat
    18721 gtcggacctg gaggcggtgc cgaacccggt catccattcc tgccaagtcc tcgacgcggg
    18781 ccgagacgtg caccgcatcg agatcgtgtc cgaggagctg aagcgcggcc gccagatggg
    18841 gtacagccgt tcgaagatcg tcgatgcgga tcagccgacc cgggtcctcg ccctgaccga
    18901 gggccagggc atcagcatcg gcacgccgcc cgagggactg cagaagatgt cggtcgagcc
    18961 catcgaggtg accgactcgc ccgaactgcc gccgctctgg caggtgttcg gcgggcaccg
    19021 gcgcgccgac ggccgttggg tgctgccaga actagacgac aaggtggcgt cccctgacgc
    19081 tgcgttgcac atcggaccgc agttcgtcat gttggaaacg gctgcgctcg acttggccgc
    19141 cgatatcgcg ggaaccgatc agctccaagg tgtttcgtcg cacacaatgt tcgtcgcgcg
    19201 cggcaaagtc ggcccgttcc gcgtcgccgg cgaacctttc gccggcgcgg acggcacggt
    19261 tgcggtccgc gtcgtgatgc acgacgaggg agccgacgac cgcgtgacca ccgtcgggtc
    19321 gtacctgttc cgggtcgagg tgtgactcgt ggaaacctgg gagctggtgg cttgtgagcg
    19381 gatccgggat accctcgccc gctacaactg ggccggtgac tcgttgcggc tcgatgaatt
    19441 cgccgaaacc ttctgcgagg acggcgaatt gcaactgcgg ggcgacgagc cggtgcgcgg
    19501 acggacggcg atcgtcgcgt tcatcggcgg cgtcgcgaca tcggacgcac cggcgtcggg
    19561 gccgccccgc atcgtgcgcc acaacctgac gaacatccga ttcctcgagg tgactccgca
    19621 gcacgcgcgt gtcgcgtcgt acttcaccgt cttcaccgat atcgggctgg atcactacgg
    19681 ccggtaccga gacacgctcg tgccggtcgg tgagaagtgg ctgatccggc accgcttcgt
    19741 atcgacggac tgggcggcgc cgaactcgac gatggcagaa ccgcaaatac ggtcctaacg
    19801 ctctcaattg accgcatctg cggctacgct tcacccatga ccgcgattcg ggtccgtgag
    19861 gcggccgaac tactgggagt cagcgacgac agcgtccggc ggtggatcga cgacggaagc
    19921 ctgcccgctg atctcgaccg atccgggcga aaggtcatcg caggcgacgt ccttgccgag
    19981 ttcgcccgcg cccacgccac gacaccgccc aaggatccgc tcagcgtcgg cagctcggcg
    20041 cgcaacaggt tcgccggact ggtgaccaag gtcgtcgccg acggggtgat ggcgcaggtc
    20101 gaaatgcaat gcgggccatt cacggtggtt tctttaatga gctccgacgc cgcccgcgaa
    20161 ctgaatctga aaccgggcag cgtcgccgtg gccgtcgtca aggccaccac cgtgatcgtc
    20221 gaaacaccga aaggacagtc atgaagcact gctggcggct gacactggcg atcgtgctcg
    20281 cactcgccat cggtgggtgc ggctcagacc aaccgtcgaa gtctcctgag aagatcgtcg
    20341 tgttcgcggc ggcctccttg aacaaatcct ttaccgaaat cggtgagcgg ttcaagaccg
    20401 acaatcccgg tgcctccgtg gagttttcgt tcgcggggtc ctcggacttg gtcacccagc
    20461 tgactcaggg tgcctccgcc gacgtgttcg cgtccgccga ctccaacaac atgcagaagg
    20521 ctgcccaagg cgggctgttg gccggcgatc cggtgaactt cgcgtcgaac accctcacaa
    20581 ttgcggtcgc tccgggaaac ccaaagaaca tcagctcatt tcgggatctg acgaagccag
    20641 gtctcaatgt cgtcatatgc gcaccgcaag tgccgtgcgg gtcggcgacg gagaaggtcg
    20701 agcagaacac cggggtgcaa ttggcccccg tcagcgagga gtcgtcagtc accgacgttc
    20761 tcaacaaagt gctgaccggc caggccgacg ccggggtggt gtacgttacc gacgccatcg
    20821 gcgccggcga caaggtcacc gcggtaccgt ttcccgaatc cgccaatgca gtcaatgttt
    20881 atccgatcgc cgtactgaaa cagtccaaga atccggagct ggcacgcaag ttcgtcgacc
    20941 tggtcaccag cgaggccggc cagaaaattc tcaacgcggc tgggttcgcc aaatcgtgat
    21001 caccgagagt gacgttttgc agtccgctac tcgcactttt cctgcgaaac gtcgctttcg
    21061 cagagcgccg tagaactggg cggccttgcg tatcgagtcc ggcgtagagc ttggctgcca
    21121 acctaattcg cgcgtagcct taccgtgatc caacggcgac atgatgtgcg ccagcctgac
    21181 gctgacactg ttgatcggca gatcgcgtcg cagcacccgg gttgcgacat ctgccaggcg
    21241 ccccaaggca gacgtcagcc ggaacggaac gctgatacgc ggccgccgtg cgccgaccgc
    21301 gtcggcggcg gtgtcgaaga tgtcacgcca cgacatgaac ctttccgaca cgatgtagcg
    21361 ctcgccgttg cggccgttgt ccgcggccag cacaagggct tccgccgcgt cctcgatccc
    21421 caccacttca taggccaggc cttggaagtg aagcggcatc cttcccgccg ccaccaacgc
    21481 gacgaggctg ccgtgcgggg tcggctgcca gtcgcgcggc ccatacgtgt tggcgacgca
    21541 catcaccaca gcgggcaatc cccgttcgca tgcatgctgc accacgagat tctctgcctg
    21601 gacacgggat tggatgtagc cgccgccgaa ttcgtaccag ttgaacggca ggtcctcggt
    21661 ggcgggaccg tcgtcgctga gcgcaatcgt gccgatcgtg ctggtgaaga cgaaccgttt
    21721 gaggttggct tcgacggcag cgtcgagcac atggcgcagg ccctcgacgt tggtctcgaa
    21781 cagcggagcg gggtcgcgca gccgacatac tgctccttcc ggtccattgg cggaaatcta
    21841 tactgtaatg tattcagtat caactgagcg gagggtcgtg gcgtgaaagt cccgttcacc
    21901 tggaaggtca ccggctggtt catgatcggc tggtcggccg agttccccgc cggggaggtg
    21961 cgaccgctgc actacttcgg tgaagacctc gtcgcctacc gtgacgagtc gggcgagctg
    22021 cacgtcctgc aggcgcactg ccgccacctc ggcgcccaca tcggccacgg cggcaaggtc
    22081 gtcggcgact gcgtcgagtg cccgttccac gggtggcggt ggggtcccga tggcaccaac
    22141 cgctacatcc cgtaccagcc cgaccggccc aaccgggcgt tgacgctgcg cgtctacccg
    22201 gtcatggagc agtacggctg cgtgttcgcc tggcaccagc cgcagggcaa agagccgcag
    22261 tgggacatgc cggacatctt cacgtcgttc ccgcaattcg agaccgaccc ctcggcgtat
    22321 taccgtccct atccggagtt ttcgcggcgc acggagaacg aggcggtgca tccgcagatc
    22381 gtcgccgaga acggcccgga cagctcgcac ttcaagtacg tgcacaaggc gaccgtcacg
    22441 ccggtgtccc tcgactggcg catcgtcgac caggagtggc ggttcctcac tggctggccc
    22501 gacgtgcgcg ccgacgaccc ggacaagatg gccctgcgga tccacagcca catgttcggc
    22561 ctcggcggcg cgatcagcgc gttcgaaggc tcgtcgaacc atcgcctgat cttcgcggtc
    22621 acaccggtcg acgacggctc ttcgacgatg ttctattcga tctggtggcc gaagctcgac
    22681 ggggagacct ccgacgtgcc gcccgaggcg gtacggaagc aggtggagaa gcagttcttg
    22741 cgcaccgtct gggaggacct ggacatctgg cgctaccagg agtacatcga gcgcccttcg
    22801 ttgcccaagg tggacgcgaa accctatatg gcactgcgga agtgggcgac tcagttctac
    22861 gagaccccag catgacggtc gatctgcccg aattgattgc acccggacac accgcgattg
    22921 tcacccagga gtgccagggc gcggtgatgg gcccgaacgc gggactggcc gtgttcgcca
    22981 aggaagcacg ccgcgaggcg ctgccgaaca tcgtccgtct gctcccggcc gcccgcgcgg
    23041 ccggcgtgca ggtcgtgcac tgcctggtgc agcgccgccc cgacgggctc gggtccaacc
    23101 acaacgcgaa aatcttcgcg gtcgggcgca cggaggtcga catcactccg ggcagcgagg
    23161 gtgcgacgct attgcccgag ctcggaccgg aacccaccga tgtcgtgctg caccgctggc
    23221 acggcgtcgg gccgatgggt gggaccgatc tggacgcgaa cctgcgcaac ctgggagtgt
    23281 cgacgatcgt ggcggtgggc gtctcgctca acgtggcgat ccccaacctg gtcatggatg
    23341 cggtgaacgg cgcctaccgt gtggtgctcc cgcgcgacgc ggtcgcgggc ttccccgccg
    23401 actacggcaa cgcggtgatc gacaactcgc tgtccctgct tgcgacgatc acgaccacgg
    23461 atgatctact ccgagcatgg acgacatcgt gagcttcacc gttccggcgg tcgcacaagc
    23521 cgtcgcgacc gcaatcggcg accgcgtcct cgtcacccag ggcgacaagc agtacacgta
    23581 tgcgcagatc gtcgagcggt cgacgcggct cgcgtcgtac ttgcactcgc gcggactggg
    23641 ctgccacacc gagcgctcgt caatcggagg gcacgaggtc ggccaggacc tcgtcggcat
    23701 ctacgcgcac aacggcaacg aatacgtcga ggcgatgctc ggcgcgtggc gggcacgagt
    23761 ggcgccgttc aacatcaact acaggtacgt gaaggcagag ttgcagcatc tgctcgccga
    23821 cgccggcgcc actgcgctga tctatcacgc cgcgttcgcg ccgcgcgtcg ccgaggtgct
    23881 acccgacctg cccaacctga aggtgctcat ccaaatcgcc gacgactcgg gcaacgagct
    23941 actcgacggc gccgtcgact acgagaccgt gatcgcgtcg agttcgtctg agccaccgcc
    24001 ggttcagccg tcgccggacg acttgtacgt cctgtacacc ggcggaacga ccgggatgcc
    24061 gaagggcgtg ttgtggcgac agcacgacat cttcgtgacg tcgttcggcg ggcgcaacct
    24121 cgtcaccggc gaagtcatgg gctcctacga cgagatcgtc gcgcgtgcgg ccgagaaccc
    24181 gggaacgaag atcatgatcc tgccgccgtt gatgcacggc gccgcccagt ggggtgtgat
    24241 gaccgcgatc accaccgggc agtcactggt cttcgcgtct gtggtcgacc ggttggacgc
    24301 cgccgaggtt gttcgcacga tcgagcgtga gcgcgtgtca gtggtgactg tggtcggcga
    24361 cgcgatcgcc cggccgcttc tcgcggcgat cgagaaaggc acggccgacg tctcgtcgtt
    24421 ggtggcgatc gccaatggcg gtgcgctgtt gaccccgtcg atcaaagagc gcttgatcaa
    24481 gacccttccg aacggcatgg tcatcgacgg cgtcggctcc tcggagaccg gcgcgcagat
    24541 gcatcacatg tcgatctccg gggcggtgtc gaccggcagg ttcaacgccg gaccggacac
    24601 cttcgtcgcg gccgaagacc tgagctcgat tctgccgccc ggccatcagg gcatcggctg
    24661 gctggcgcag agaggctatg tgccgcttgg ctacaagggc gacgcggaca agacggcggc
    24721 cacttttcct gtcatcgacg gcatgcgata cgccgtgccg ggcgaccggg cacgccacct
    24781 cgaggacggg tccatcgagt tgctaggccg cgactcggtg acgatcaatt ccggcggcga
    24841 gaagatcttc gccgaggaag tcgagatggc gatcgcgtct catccggcgg tcgcggacgt
    24901 ggtggtggcc ggccggccaa gtgaacgctg gggccaagaa gtggtggctg tcgtcgcgct
    24961 cgccgacggg gcgagcgtgg acgcgtcgga gttgatcgag catgcggcac agtcgatcgc
    25021 gcggtacaag ctgccgaagg ccgtggtgtt ccgcccggcg atcgagcgca gcccggccgg
    25081 caaggcggac tatcggtggg cccgcgaaca ggcggtcagc gacgcgtaag tgactgcatc
    25141 ccaggcgcct caccaacccc atcggcctct tggggggacg ttgatgcttt tgcccgcatc
    25201 tgagcgacaa tcgcgttctg ccaggcccag cctccgacga gtgttgagga ctcagcgaca
    25261 acctgcggct gcaacgcagg tcagcgggtg tcgcttggag gcgggcaatt cggataatgc
    25321 catggccggt gacgcacgtg acggatgtga cgcgcggcta cgtggaaagg actgtggcag
    25381 cggctgttcc gggtgcgccg tagagctggg cgaaaccgac ccgcggctct ccgggcacct
    25441 gacgctcgcc tacctcgccg cgcagctgcc gcaccagctc gtggatctgc cgcagcccgg
    25501 acgcaccgat cggctccccg ttggcgatca gcccgccgtc ggtgttgacg ggcatcgcgc
    25561 cgccgatttc tgttgccccg tcggccaata gcttctcctg gtcgccgtcg acgcagaacc
    25621 cacattcggc catgtggatc acctccgcgc cggcatcggt gtcctgcagc tggatcacgt
    25681 cgacgtctcc cggtgccacc ccggccttgt cgaatgcggc gcgtgccgcg tacaccgtcg
    25741 gcgacacgtc ctcgtcaacc ggcgcgaacg tggtgttcac ctcgtaggcg ccgtagcggc
    25801 gagtccggac ctccgccgcc cgcaggtaca cgggtttgga ggtgtaacgg tgagcgatgt
    25861 cgccgcggca cattaccacc gcggcggcgc cttcgtcggg cgcgcagaac atgtactggg
    25921 tgagcggata gttcagcatc ggtgagttca agatctggtc ttcgggaatc ggcttgcgcc
    25981 ggaacgcatt cgggttcagc gcgccgttgc ggaagttctt ggctgccact ttggccagag
    26041 tctgctcgga gatgccgtgc tcgtgaaggt agcggttggc cttcatcccg aagaacttgg
    26101 tggtgaggta ttgcccgttc tgcgcgtacc agctcggcat cccgaccaat gcgggatcct
    26161 cggtgaacgc gccgcgtgga tgcttgtcca taccgacggc gatgccgatg tcgtaatcgc
    26221 ccaaccggat accgtccgcg cacgccttgg cggcgctggc cgcggtcgcg caggcgttga
    26281 acacgtctgt gaacgggatg ccggtgagcc cgaccagact cacgatcgcg tcggggttgg
    26341 cgacggtcca gctgcccccg acaccaaact gaatgtcctt ccaccggacg ccggcatcgg
    26401 cagcggcggc gaagatcgcg tccgccccca tctgcatcgc ggacttgccc tcgaaccgcc
    26461 cgaacgggtg caggcccacc ccgatgatcg ccacgtcatt catgaagcaa cgtcctctcg
    26521 tgtcacgacg aaacataccg gacctcaagc cgctacttat actgtaatct ttacagtagg
    26581 atcatgtagc ccagacctga caacgctcgg agaccagccg tggagacgca agcccaggaa
    26641 atcctgtcag ccgaccgcgc cgcggaatgg aagcgcgatc cctatccgtt tttcgccgag
    26701 cggcgccgac aggcaggcgt attccggggg cccgtcatcg actactccag gcttccggag
    26761 tcgatgagac caaagcatgt gttttcggcg ttgtccttcg acgcggtgaa tacggtgttt
    26821 cgcgacggcc gcgtcttcga ctcgagcggc tacgacacgt cgatcgggtt gttcactggc
    26881 aggaccatcc tcgccatgga gggcaaggag caccgcgagc accgcaactt ggtgtcgacg
    26941 gctttcaaat ccaagtcgct ggcgcgctgg gaaccagaaa tcgtccgtcc tgtctgcaat
    27001 gcgttgatcg acgaattcgc cgagagtggt gaagccgatc tcgtgcgaaa gttcacgttc
    27061 gagtttccga cccgtgtcat atcgaggttg ttgggcctgc cagaggacga cctgccgatg
    27121 ttccggcggc gtgcgatcca actcatcagc tacaactacg attacaagca ggcctttgaa
    27181 gcatcggctg cgttgaagga gtacttcctc gagcagatcg agcaacgcaa atccaagccc
    27241 accgaggaca tcatcggcga cctcgtcacc gccgagatag agggcgagaa gctcagcgac
    27301 gaagcgatct actcgttcct gcggctactg ctgcccgcgg ggttggagac gacctaccgc
    27361 tcgtcgggca acttgctctt caacctgctg acacgtccgg accagttcgc ggccgttcaa
    27421 gccgaccgcg acttgattgg cccggcgatc gaggagaatc tccggtacga gacaccgctg
    27481 acgtcggtgc agcggttcgc gaccgaggat acggagctag aaggagtcga gattcccgcc
    27541 gactccatgc tcgacgtctg cattggctcg gcgaatcgcg acgaacatcg gtgggaaagg
    27601 tccgaggagt tcgacatctt ccgcaagccg atcccgcaca tctcgtttgc ggtgggcgaa
    27661 cacacctgca tgggattgca tctggcgcgc ctggaaaccc gcgtggcctt agagtgcctc
    27721 ctggaccggt tgaccaacat tgcgttgatc accgacgacg acccacacat ccgcggcaat
    27781 ccgttccggt cgccgacggc attgccggtc actttcgacg tcgtcggctg atgccgcttc
    27841 atcctttcca gggcaccatc ttcacgtagc cgcccgcatc gacgcgcaat tgcgagccgg
    27901 tgatgaaccg cgcatcgtca ctggccagga acaccacggc gttggagacg tcctgcggct
    27961 cgacgtacgg aaccggcatc gcctgcatga ccggaaacac cgcttcggcg tcttcgcgtg
    28021 tcggcgactc cttgtccggc cggaattgct gatacatgcc ctcgttctgc agcatgtcgg
    28081 tgttcacgtt ggccggatgc accgcgttga cccggatcat ccggttcgcc agctggatcg
    28141 acaggtcgtt gacgtagcgg gcgacgacct gcttggccaa gctgtaggcg gagctgcccg
    28201 ggccctggtt cgttgcgtta ccgatcagtg cggcaaggga tcccgttgcg atgatcgatg
    28261 cgccgtcgtt gagatgcggc aggctgccct ggatcacgtt gaacacgccc acaagatcgg
    28321 tgtcgatggt gtccgaccac gactggatgg tctgcccctt gcccatcgcc gcgatgccgg
    28381 cgttggccac cacgacgtcg aggtgtccga gttcgttcac cccctcctcg atcgccttcc
    28441 acacaggcgt ccgctcgtgc acgtcgacga tcgcggtatg cactggcaca ccttcctttt
    28501 cgaccagccg cgcggtctcc tcgaggtcct ccggccgcgc cagcggatac gtgctgccgt
    28561 cgagatccgc gcagatgtcc agcgcgatga tgcccgcgcc ctcggcggcc aagctcaccg
    28621 cgtgtgaacg gccctggccg cgcgcggcgc ctgagatcac cgcgaccttg ttgtcgagct
    28681 tccccactac tgttgccttt cccgtgttcg tggcatgcgg catccgatgg tcttggtttc
    28741 caggtactgc tggaaacctt cgatgccgca ctgccggccg atgccgctgc tcttgtaccc
    28801 gccgaacggc gcgtcggcgc cgtagaacat tccgccgttg acgccgatcg acccggtccg
    28861 gatccggcgc gcgatgctca ttccccgttc cgtcgatgcc gaaagcaccg ctcccgcaag
    28921 gccgtacaag gtgtcgttgg cgaggcgtac ggcctcgtcg tcgtcggcga acggaatgat
    28981 caccagcacc ggtccgaaga tctcctcctg ggcgatcgcc gcgctgttgt cggcaccaac
    29041 gatgaccgtg ggctgcacat agtggccacc cgccaagtga gccggcagat cgtcgaaaga
    29101 gcccccgccg gtggtgatct cggcgccgtc gcgacgcgcc tgctcgcatg cggacagcac
    29161 gcgctgcttc tgcgtcgcgc tgatcaccgg tcccaccagc gtgtccggca gggccgggtc
    29221 acccaccgga accatctgga acactgcggt cacgttggcg acggcctcgt cgaacaggga
    29281 ttcgtgaatc agcatgcggg tggtgagcgc gcacgcttgg ccggcgtgca cgcagacccc
    29341 gaccgcgctc gggacgacct tggcggggtc cgcgtcgtcg agcacgatca acgccgactt
    29401 cccgcccagc tccaggaagg tgtgcttcat ggtgtcggcg ccctggcgga ccagcatctt
    29461 gcccacggcg gtggatccgg tgaacgagat catgtccacg cgcgggtcgg tggccaagat
    29521 ggcggccacg tcgttggacg gcgtcgagac gacgttgacc acacccggcg ggatgtcggt
    29581 gtgttcggcg atcagccgcc ccagccgggt ggcgttccac ggcgtgtgcg gatcgggttt
    29641 gagcacaacg gtattgccgg tggccagcgc gggaccgagt ttgttgagga tgacctcgat
    29701 cgggaagttg gacggagtga tggccgcgac gacaccgacc ggctccttca ccaccgtccg
    29761 cacgttgcgt tcgccgaaca gcccaccgcc atccagggtg cgctcccatt cgaagtcgtc
    29821 gatcagcctg gcgggatagc gcaacgactc ggccaacggc cagtcgacct gcgcgctgtg
    29881 cgtcgtcatc accgggcagc cgacctcggc gatgagttcg gtgcgaaggt cttccttctc
    29941 ggcttcgagg gccgactgca actggagcag ccagcgcttg cgcagttcgc ggttagtcga
    30001 ccagtccgtg tcgtcgaaag cccgccgcgc cgccgcgatc acccggtcca tgtctgctac
    30061 ctcagccgcg gctgtgcgtc ccagcaccag gccggtggcg gggctgacgt tgtcgaactc
    30121 ggcgccggag actgatgcgg tgagatcccc gtcgatgagc atccgcgatt cggcgcgctc
    30181 cgccacgcgg cggccgacct cgatgctcgg cgcggcctgg tcctgcgcga cgtcgctcac
    30241 ctgcacaccc tcctgatctg gggcgatcaa cggtaaacat tacagtagta tttttcaatg
    30301 tgatcaaggt gatggagggc ttccgggtcc tggagatagc acagttcacg ttcgtcccgg
    30361 cagcgggcgc gattctcgcc gactggggtg cggacgtcat caaggtcgaa catccggtac
    30421 gcggcgacac gcagcgcggc ttcctcaaca tgggcggcca cgagctgaac ccggacaggc
    30481 atccgctgat cgagcacccc aaccgcggta agcgcagcgt cggtatcgac gtctcgaggc
    30541 ccgacggcca agaagtgttg tacgagctcg ccaagaccgc cgacgtgttc ctgaccaact
    30601 acctgcctgc agcgcgtcag aagaacaagt tcgacgtcga acacatccgg gcggcgaact
    30661 cgaagatcat ctacgcgcgt ggcagcgcct acggcgacaa ggggcccgag cgcagtgtcg
    30721 gcggctacga cgggacggcg ttctggactc gcagcggggt gggccactcg ttgacaccgc
    30781 cggagctcgg cggcatgctg tcgcaaggga ttccggcgtt cggagactcg atcggtggca
    30841 tgaacatcgc cggcggcatt tcggccgcgc tgctgcatcg agagcgaacc ggcgaagccc
    30901 tggaagtgga tgtgtcactg ctcagtacgg cttggtgggc ggcgggagcc agcgtgacgc
    30961 acggcatgga caccggcgag accatgcgcg cggaaatgcc acattccggc ggctcggccg
    31021 tgaatccgtt cctgggtaac tatgaaacct ccgacggcgg cacgatcaac ctgtgcatcg
    31081 tgagcccgac cggctacatc cgcgacacgt tcgagcacct cggcatcccg gaggctgccg
    31141 acgatccccg cttctccgac gtgctgccgc tgatggagaa cgccgaggcg gcaagcgaat
    31201 tgatcgtggc tgcgatcggc agcaagccgt tcgagtactg gcgtcagcat ctgaagacga
    31261 tgaaaggcca atgggcaccg ttccagagct tcatcgacct agccaccgat gagcaggcca
    31321 tcgtcaacga catggtcgtc gaggtggaac ccgccgacgg cggaaagcca ttcaaagtcg
    31381 ttcgcggacc ggtccagttc aaccacgagc ctctggtgac cacgcgcgcc ccgcaggcat
    31441 ccgaacacac cgaactcgtc ctgatggaac tcggcatgga ctgggaccgg attgcggcac
    31501 tgaaggattc aggcgcgatc gcctaacacc ccggtatcac gagggagcgc tcaggcgcgc
    31561 tgcacctcga ccggtacacc ggtcagccac gacattccgg ccagcgactc caggtcgtcc
    31621 ggatcgctcg gcgtcaactg gttcacgttc gcgccgcggg cgcggttggc cagctgccag
    31681 ccgccggtcc ctttgtggcc ccagccatgt ggcaccgcga tggtgccggg gacgatgtcg
    31741 gcggtcaggc acaccggcaa cgagatgcgg ccgtatggag agctgaccgc aacggtgtcg
    31801 ccgtcggcga tccggcgcgc catcgcatcg tcgcggtgca tcagtgcgcg gtgaaatcgg
    31861 ttgctgcgca tcaacagcgg cgcgttgtgc atccaggagt tctccgagcg gggctcgcgc
    31921 atgccgatca tccgcatcgg ataatccgca ggctcgctgc gctccgacaa tttggcgatc
    31981 tcctcggcga tgtcctcgtg tgccagtcgc acccggccac gccggtagac caccacctcg
    32041 cccagcacgc ctgtccggat atgcggtgcc acgacggcgc cgtggggatg ctcagaaatg
    32101 agtcgggaaa acgacagccc gccgcgacgg agaccgaacc tgtcccctcc ctcggacatc
    32161 cggatcatca tgtcgatcag tggacgggga tgcaggcgcc gaccgaacaa ccccaagagc
    32221 cggcgaccag ccgacaatcc cgcgacgacc ggcgcgcgga ttgccgttcg tttcatcaac
    32281 tcgtcgatga tctcccactc cgagcgcgct tggccgcgtg gtggcaccac cgcctcggtg
    32341 gcctgccgaa acggcgtcgc ttggaaggtc tgaaaggtca ccgcgaagtc gtcacgctcg
    32401 tacatcgtgg tgacgggcag gatgtagtcg cagtgcgccg tcgtctcggt cacatacaag
    32461 tccagcccga ccatcagctg aagagtttcg agtgcctctt ctagttcatt gccgttcggc
    32521 acggacagca ccggattgcc cgcgctgacg aacagcgcct tgatctgccg cgttcccgtg
    32581 gtggtgattt ccttggccat caacgcagcc ggttcggaac cgataaccgc tccgaacccg
    32641 cccacacggg tgcgtttgcg ccggtaggcc cggcgtaacg acgcgcccat cgccatcgtt
    32701 cccagcttct gacccggcat ccccaatgtg ctgaacacac ttcctccggg gcggtcgagc
    32761 ttgcccgcga ccagattgac cgcgtcgatc agatacgcgg tcagtgtgcc ggaccttccc
    32821 acacacgtgc cgaaccggcc gtacaccgcc gcgcgcggcg tatgcgccag atcgcgtgcc
    32881 aacgttcgga ccacatctgg ttcgatgccg gtcatcgacg cggtgggttc gggcggaaaa
    32941 gc
//
LOCUS       DASWBF010000233         5001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_680994,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000233 DASWBF010000000
VERSION     DASWBF010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680994"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /gene="egtA"
                     /locus_tag="VF874_08440"
     CDS             complement(<1..1225)
                     /gene="egtA"
                     /locus_tag="VF874_08440"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis glutamate--cysteine
                     ligase EgtA"
                     /protein_id="HEX9176109.1"
                     /translation="MAETTFAVTSGVDHEPLDSLTSAADHVAATCLADTPVGLVGLEL
                     EAHCFDVADPSRRPGWDELCGVINSVPELPGGSRVTVEPGGAVELSTPPVRGVVAAID
                     ALAADRAVLRTVFAKAGLALVLLGTDPLRPARRVNPGDRYQAMERYFTAAHTGAAGAA
                     MMTSTASVQVNVDAGPADGWADRVRLTHALGPTMIAMSANSPLLAGSFSGWRSTRQRV
                     WGQLDTARCGPILGASGDDPASDWARYALRAPVMLVHEPVPVPVTRHVAFADWVDGQV
                     RLGGRRPTTADLDYHLTTLFPPVRPRRWLEVRYLDSVPDTLWPAVVFTLVTLLDDPAA
                     ASIAAEATEPVATAWDVAARVGLADRRLHASATSCVQAAAERVPPALQDSMQRLVRTV
                     EEASCPGEEFAERAIR"
     gene            complement(1289..1933)
                     /locus_tag="VF874_08445"
     CDS             complement(1289..1933)
                     /locus_tag="VF874_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX9176110.1"
                     /translation="MRLAAAAASVSVGLAIGLAAPAYAVPSEPGVVNYAVLGKGSVGN
                     IVGSTMRWENFFTEPFQGYWVDLAACNNWADIGLPEVYLDPDLASFNGAETKESATEE
                     THFVKQAVGVFATNDAADRAFHRVVDRTVGCAGQTTGMHFADGTTQVWSFSNGLVNAT
                     DAEWVKQEAGTDRRCFTQTRRRENVLLQAKVCQSGNGGPAVNVLAGAMQNSLGQ"
     gene            complement(1938..2366)
                     /locus_tag="VF874_08450"
     CDS             complement(1938..2366)
                     /locus_tag="VF874_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX9176111.1"
                     /translation="MSIEVVYTAESTASGGGRDGHVKSSDGKVDLDTRPPKEMGGSGE
                     GVNPELLFSAGYAACFLGALRLVARNDKVDLDDATAITARVGFGKDSEGGFGLTGQLI
                     GYLPGLEQDQADDLMEKAHQVCPYSKATRGNIDVELSAKV"
     gene            complement(2459..3919)
                     /locus_tag="VF874_08455"
     CDS             complement(2459..3919)
                     /locus_tag="VF874_08455"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX9176112.1"
                     /translation="MTDKYATNDAGNPVPSFEHSLTVGPDGPILLQDHYLIEQMANFN
                     RERIPERQPHAKGGGAFGHFEVTQDISTYTKAAVFQPGTKTDTVIRFSTVAGERGSPD
                     TWRDPRGFALKFYTSEVNFDMVGNNTPVFFVRDPMKFQNFIRSQKRMQANNLRDHHMQ
                     WDFWTLSPESAHQVTWLMGDRGIPKTWRHMNGYSSHTYSWINAAGELYSVKYHFKTDQ
                     GVEFLTQEEGDEMAGTDGDAHQRDLYESIERGDYSSWTLHVQIMPFEEANTYRFNPFD
                     PTKVWPHGDYPLHEVGRLTLDRNVTDYHAEIEQAAFEPNNIVPGTGLSPDKMLLARGF
                     SYSDAHRARLGVNYKQIPVNTPKVEVHSYSKDGAMRIRNVTDPVYAPNSMGGPEADPR
                     RAAEVHWASDGEMVRAAYALRQDDDDFGQAGTMVRDVLDDAARDRLAHNIIGHISKGV
                     KEPVLSRVFEYWKNVDPDLGKTIEEGVRANLNGNGA"
     gene            complement(4263..4658)
                     /locus_tag="VF874_08460"
     CDS             complement(4263..4658)
                     /locus_tag="VF874_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176113.1"
                     /translation="MSETAEPDTTPTLTTDPPPAPPVHRDVPDRSRPSRLFQALAWVG
                     IVAGAVFIVAVVFFSGFLIGKHSGPGYDHLRGHDGPHMLHRGGPSAPMGPVGEWPGPG
                     RMGPGGPMGPPTRPGNAPTTTAAPTPPRP"
BASE COUNT          792 a         1664 c         1661 g          884 t
ORIGIN      
        1 aacggatcgc gcgctcggcg aattcctctc cggggcagct ggcctcttcg acggtacgga
       61 ccagccgctg catcgagtcc tgcagcgcgg gcggcacccg ctccgcggcc gcctggacgc
      121 agctggtagc gctggcatgt aagcgccggt cagcgagtcc gacccgggcg gcgacatccc
      181 acgccgtggc caccggctcg gtggcctccg cggcgatgct ggccgcggct gggtcatcga
      241 gcagggtgac aagagtgaac accaccgcgg gccacagcgt gtcgggcacg ctgtcgaggt
      301 agcggacctc cagccagcgc cgcgggcgaa ccggaggaaa cagcgtggtc aggtggtagt
      361 cgaggtcggc cgtcgtgggt cggcgcccgc ccaggcggac ctgcccgtcg acccagtcgg
      421 caaacgcgac atgcctggtg accgggactg gaaccggttc gtggaccaac atcaccggcg
      481 cccgcagcgc gtaccgcgcc cagtcgctgg ccgggtcgtc accgctggca ccgaggatgg
      541 ggccgcagcg agcggtgtcg agctggcccc acacgcgctg gcgggtgctg cgccacccgg
      601 agaacgaccc ggccagcagc ggcgagttgg ccgacatcgc gatcatcgtc ggaccgaggg
      661 catgggtcag tcgcacacgg tcggcccagc cgtcggccgg gccggcgtcg acgttgacct
      721 gcaccgacgc ggtcgacgtc atcatcgccg cacctgccgc gcccgtgtgc gcggcagtga
      781 aataccgctc catcgcctgg taccgatcgc ccgggttgac gcggcgagca ggccgcagcg
      841 ggtcggtccc gagcagcacc agcgcaaggc cggccttcgc gaacacggtc cgcagcaccg
      901 cccggtcggc ggccaacgcg tcgatcgccg cgacgacgcc gcgtaccggc ggcgtcgaca
      961 gctcgacggc gccgcccggc tcgaccgtga ctctgctgcc accgggcagc tcaggtacgg
     1021 aattgatgac cccgcacagt tcgtcccacc ccggccggcg ggaggggtcg gccacgtcga
     1081 aacagtgcgc ctcgagctcg aggccgacca gccccaccgg cgtgtcggcg aggcaggtgg
     1141 cagcgacgtg atcggccgcc gaggtcagcg aatcgagtgg ctcgtggtcc acccctgacg
     1201 taaccgcgaa cgtcgtctcg gccatccctc catcttgcag accccgccga caagttccga
     1261 gtctttggcg tgacaggtcg atgacgggct actggcccag cgagttctgc atcgctccgg
     1321 caagcacgtt caccgcgggt ccgccgttgc cggactggca gaccttcgcc tgcagcagca
     1381 cgttctcacg ccttcgggtc tgggtgaagc agcggcgatc cgtccccgcc tcctgcttca
     1441 cccactccgc gtcggtcgcg ttgaccaatc cgttggagaa cgaccacacc tgcgtcgtcc
     1501 cgtcggcgaa gtgcattccc gtggtctgcc ccgcacatcc gacggtgcgg tcgaccacgc
     1561 ggtggaacgc gcggtccgca gcgtcgttcg tggcgaacac gccgaccgcc tgcttgacga
     1621 aatgcgtttc ctccgttgcc gattccttcg tctcggcgcc gttgaacgac gcgaggtcgg
     1681 gatcgaggta gacctcaggc agcccgatgt cggcccagtt gttgcaggcg gcgaggtcga
     1741 cccagtagcc ctggaacggc tcggtgaaga agttctccca ccgcatcgtc gaacctacga
     1801 tgttgcccac cgatcccttg ccgaggaccg cgtagttgac gacgccaggc tccgacggca
     1861 cggcatacgc gggcgcggca aggccaatcg cgagaccgac gctgaccgat gcggcagcgg
     1921 ccgccagtcg caccgaacta gactttcgcc gacagctcaa cgtcgatgtt gccgcgggtg
     1981 gccttcgagt acgggcagac ctggtgggcc ttctccatca gatcgtcggc ctggtcctgc
     2041 tccagtccgg gcaggtagcc gatcagctgg ccggtcaggc cgaagccgcc ctcggaatcc
     2101 ttgccgaaac cgacgcgcgc agtgatggcc gtcgcatcgt cgaggtcgac cttgtcgttg
     2161 cgagcgacca gccgcagagc accgaggaag cacgccgcat accccgcgga gaacaacagt
     2221 tcgggattga cgccctcacc gctgccgccc atctccttcg gcgggcgggt gtccaggtcg
     2281 accttgccgt ccgacgactt cacgtggccg tcgcggccgc cgccggatgc cgtggattct
     2341 gcggtgtaaa cgacttcgat gctcatggtc ttgatcatgc caacacgctc accgaaacag
     2401 aagccagggc tgttactcga cgaatttcac agccctggct tcagtttcga cggaaaggct
     2461 aggcgccgtt gccgttcagg ttggcccgca cgccctcctc gatcgtcttg cccagatcgg
     2521 ggtcgacgtt cttccagtat tcgaacaccc gcgatagcac cggctccttg acgcccttgg
     2581 aaatgtggcc aatgatattg tgcgccaagc ggtcccgtgc tgcgtcgtcg agcacatcgc
     2641 gcaccatcgt gccggcctgg ccgaagtcgt cgtcgtcctg acgcagcgcg tatgcagcgc
     2701 gcaccatctc accgtcggac gcccagtgca cctcggccgc tcgtctcggg tcggcctccg
     2761 ggccacccat cgagttcggc gcgtacaccg ggtcggtcac gttgcggatc cgcatcgcgc
     2821 cgtccttgct gtagctgtgc acctcgacct tgggcgtgtt caccgggatc tgcttgtagt
     2881 tgaccccgag tcgggcccgg tgcgcgtctg agtaggagaa cccgcgggcc agcagcatct
     2941 tgtccgggct caggccggtg cccggcacga tgttgttggg ttcgaacgcg gcctgctcga
     3001 tctcggcgtg gtagtcggtg acgttgcgat ccagagtcag cctgccaacc tcgtgcagcg
     3061 ggtagtcgcc gtgcggccac accttggtcg ggtcgaaggg gttgaaccgg taggtgttgg
     3121 cctcttcgaa cggcatgatc tgcacgtgca acgtccagct tgagtagtcg ccgcgctcga
     3181 tcgactcgta caggtcgcgt tggtgcgcgt cgccgtcggt gccggccatc tcgtcccctt
     3241 cctcctgggt gaggaactcg acgccctggt cggtcttgaa atggtacttc accgagtaca
     3301 gttcgccggc ggcgttgatc cagctgtagg tgtggctgga gtagccgttc atgtgccgcc
     3361 aggtcttggg gatgccgcga tcgcccatca gccacgtgac ctggtgcgcc gactccgggg
     3421 atagcgtcca gaaatcccat tgcatgtggt ggtcgcgcag gttgttggcc tgcatgcgct
     3481 tctggctgcg gatgaagttc tggaacttca tcgggtcgcg gacgaaaaac accggcgtgt
     3541 tgttgccgac catgtcgaag ttgacctcgc tggtgtagaa cttcagcgcg aagccgcgcg
     3601 ggtcccgcca cgtgtctggg ctgccacgct cgcctgcgac ggtggagaac cggatcaccg
     3661 tgtcggtctt cgtgccgggc tggaagaccg cggcctttgt gtaggtgctg atgtcctggg
     3721 tgacctcgaa gtggccgaat gccccgccac ctttggcgtg cggctggcgt tccgggatgc
     3781 gctcccggtt gaagttggcc atctgctcga tgaggtagtg gtcctgcagc aggatcgggc
     3841 cgtcggggcc gaccgtcagc gaatgctcga aactcggcac cggattcccg gcatcgttcg
     3901 tcgcgtactt gtccgtcatc tcgatctcat ctcctcattg acgattgacg tgggctgtcg
     3961 ctcggggcac tcactacagg tacccaaaaa acgacgccgg cttcgtcgac gtcgaaaccg
     4021 gcgttgtcgg gggcacttgc ggatgtcctc cgcaaattcg aagaggttgg gcggcagcgg
     4081 ttttcaacgc tgcaactcgc cgcagctacc cgcgggcccg cgtgcccccg ggcggtagcc
     4141 atcgaggtca cgtacacaag tatgcgcccg ttctggactg agtcaagaaa acgactggtt
     4201 cgccgagcgt gcgctggccg cgaaaaattt cgcagtaccc gcacgctcgg cgagcgagac
     4261 gactacgggc gcggcggggt cggtgctgcg gtggtcgtcg gcgcatttcc gggcctagtg
     4321 gggggaccca taggtccgcc agggcccatc cgacctggtc ctggccactc tcccaccggt
     4381 cccatcggcg ccgacggacc accgcgatgc agcatgtggg ggccgtcatg gccgcgcaag
     4441 tggtcatatc ccgggccgga gtgtttgccg atcaagaacc cggagaagaa caccaccgcg
     4501 acgatgaaca ccgcgccggc gacgatgccg acccacgcca acgcctggaa cagccggctg
     4561 ggtcgactgc ggtcgggaac gtcgcggtgg actggcggtg cgggcggggg gtccgtggtc
     4621 agcgtgggag tggtgtcggg ttcagctgtt tcgctcatgc cttcgatcat gccgacgctg
     4681 gtgcgccggg tgccctcgat cgccgctgtg tatttcctat gaagatggac gcccggacgt
     4741 cgagggtcgc tgatatgacc ttgatgcctg gagttggcga tcgagttcgt cgagacggct
     4801 ccgcctgtac cgcaaacagt gccttcgccg tacagcggtc aacagatcga tcttcccgga
     4861 agcctgaact atgatgtgct gctgcggccg cagatcgaca atggcacagt gttgaccctt
     4921 aagtcaccaa ctcgaggcgc tccatggaaa gcggacggtg tgaagctcga ccgccgccgg
     4981 taatccgttc ttcgccgaag a
//
LOCUS       DASWBF010000234        11106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1005593,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000234 DASWBF010000000
VERSION     DASWBF010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005593"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..375
                     /locus_tag="VF874_08465"
     CDS             115..375
                     /locus_tag="VF874_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9176114.1"
                     /translation="MSEVSSRDLNNDTAGVLRRVEAGDDITITVKGRPVAFAQRLATL
                     ESVADMESLPVDEKASRKLPVITQDDDLDGLDGAANLTIIRV"
     gene            complement(389..1864)
                     /locus_tag="VF874_08470"
     CDS             complement(389..1864)
                     /locus_tag="VF874_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX9176115.1"
                     /translation="MAEPDFAETLAARLNSFGDRPCIEFERKWYSGNEITEYIEDIAA
                     ALSRAGVPPTDPVGVVVRNRVPHAAAILGFIAARRPVAMIYSYQSPQSIARDIEQLRL
                     PAVLLDRDDWTAPVAEAVTATGAAGVMMSLDGVEVAASRRPRDDVKSDPTLAAAGLHI
                     LTSGTTGPPKRIPVKAQVLQHTVLSMTVGRTTSPDDPPELVYWPFGSIGVCQLLTGPF
                     IGKRTVLLEKFTVDEFVRAVKTYGITKAGVQPAIIRMLLDADVPKEDLASLDYLAGGS
                     APLAPAVREEFERRYGIPLLWAYGATEFAGSACALTPALDRQYGGTKRDTVGKPLPGV
                     QVRIVDPETGAEAVAGGRGLIEARIDVIGPDWIRTTDLAAVDEDGFVTIHDRADGAIN
                     RGGFKVLPETVRQALVSHPAVSDACVVGVPDRRLDEAPFAAVEVRPGSQMASESELMD
                     WVRESLPSHHVPVAITVVDELPRNAAMKVRPGDVAALYDAP"
     gene            complement(1870..2691)
                     /locus_tag="VF874_08475"
     CDS             complement(1870..2691)
                     /locus_tag="VF874_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEX9176116.1"
                     /translation="MSGRLAGKVAFITGAARGQGRAHAVRMAAEGADILAVDVAGKLP
                     SCVPYRSATPDDVAKTVRRVEDTGRRILFSVVDTRDFDGLRRAVDDGVAALGRLDIIV
                     ANAAIAAPQVWSDITPEDFRDVIDVNVTGTWNTVMAGAQKIVDGGRGGSIILISSAAG
                     IKVQPFMVHYTASKHAVTGMARAFAAELGKYSIRVNSVHPGPVNTPMGSGEMVDTVMK
                     ATETNPQLANMLTPFLPNWVAQPDDIADAVLWLASDESKNVTAARISVDQGSTQY"
     gene            complement(2688..3866)
                     /locus_tag="VF874_08480"
     CDS             complement(2688..3866)
                     /locus_tag="VF874_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176117.1"
                     /translation="MTTTREVVSDEDFQDILAQTRHFVRTAVVPREQEIMADNRVPDD
                     IRDRAKKMGLFGYAIPQEWGGLGLNVAQDIELAMELGYTSLALRSMFGTNNGIAGQVL
                     VGFGTDEQKARWLEGMASGEVVASFALTEPGAGSNPSGLRTKAIRDGGNWVISGQKRF
                     ITNAPVAQLFVVFARTRPADDEGAGIAVFLVPADADGAEVGAKDAKMGQEGAWTADVS
                     FTDVRVPDSALVGGSEDIGYRAAMTALARGRIHIAALAVGAAQRALDESVAYAATATQ
                     GGTPIGEFQLVQAMIADQQTAVMAGRALVRDAAKLWLAGEDRRIAPSAAKLFCTEMAG
                     KVADLAVQIHGGSGYMQGVPVERIYRDVRLLRLYEGTSEIQRLIIGGGLVKAAQRERG
                     "
     gene            4003..4737
                     /gene="fabG"
                     /locus_tag="VF874_08485"
     CDS             4003..4737
                     /gene="fabG"
                     /locus_tag="VF874_08485"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX9176118.1"
                     /translation="MLNGQTAVITGGAQGLGFAIAERYVAEGARVVLGDLDIGATEAA
                     TERLGGDTIAAAVKCDVTQAADVDTLASTAVERFGGLDIMVNNAGITCDATMRKMTEE
                     QFDQVIAVHLKGTWNGIRSAAAIMRDNKRGAIVNMSSLSGKVGLVGQTNYSAAKAGII
                     GMTKAAAKELAHLGVRVNAIAPGLIRSAMTEAMPQHIWDQKIAEVPMGRAGEPAEVAK
                     VALFLASDLSSYMTGTVLEVTGGRFM"
     gene            4734..5957
                     /locus_tag="VF874_08490"
     CDS             4734..5957
                     /locus_tag="VF874_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9176119.1"
                     /translation="MMTMREAVICEPVRTPIGRYGGMFKSLNAVDLGVAALTGLLDRT
                     GIAPDAVQDVILGHCYPNSEAPAIGRVIALDAGLPVTVPGMQVDRRCGSGLQAVIQAC
                     LQVANGDNDLVVAGGTESMSNVAFYSIDMRWGGARTGIRVHDGLARGRTTAGGRDYPV
                     PGGMLETAENLRRQYSISRQEQDELAVTSHQRAVAAQKDGILADEIIPVAVRTRHGEE
                     LVDTDEHPRADTSVETLSKLKPVLREEDSEATVTAGNSSGQNDAASMCVVTTPQKADE
                     LGLTPLVRLVSWAVAAVAPSVMGIGPVPATEAALSKADLRLADVDLIELNEAFAAQAL
                     AVMREWGLTAADRDRTNVHGSGISLGHPVGATGGRMLAALARELNRREARYGLETMCI
                     GGGQGLAAVFERWDQ"
     gene            5954..7144
                     /locus_tag="VF874_08495"
     CDS             5954..7144
                     /locus_tag="VF874_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9176120.1"
                     /translation="MTLPLDGITVVEVSSFVAAPLCGLTLSQLGAEVIRVDPIGGAAD
                     FRRWPLAESGRSIYWTGLNKGKRSATIDLRSLEGQELVQRLIAESEGIVVTNAAGLAW
                     LSHERLAAMRSDVISVQLLGRGDGSTGVDYTVNAAIGFPYVTGPADYAGPINHVLPAW
                     DVCCGLYAALAVTAALRRRDQTGDGTHVRLALEDVALATVGNLGLITEPQVNGTQRPR
                     LGNAIFGQYGQDFTSRDGASFMAVLLTGRHFRDLVEVTGTGAAVSALADALGTDFSSE
                     GDRYRYRDVLSGLFSTWFAEHSAAEINDALSSTTVLFERYRTFAEVATDDRVVANPLF
                     TRMHQPGVGEYFAPGMPASFDGSHPASAPAPNLGQDTAGLLAERLGLSADEITRLENA
                     GTIA"
     gene            7169..8362
                     /locus_tag="VF874_08500"
     CDS             7169..8362
                     /locus_tag="VF874_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176121.1"
                     /translation="MTRLAQTLGLTEFQTEIVFTVRQFVEKEVIPNAPELERTDTYPQ
                     DIVDQMKDMGLFGLMIPEEYGGLGESLLTYALCVEELARGWMSVSGVINTHFIVAYML
                     RQHGTDAQKQHHLPRMATGEARGAFSMSEPELGSDVAAIRTRAKRNADGNYTIDGQKM
                     WLTNGGSSTLVAVLVRTDEGADKPHRNLTAFLVEKPVGFGEVAPGLVIPGKIDKLGYK
                     GIDTTELIFDGYAASADDILGGAPGQGFFQMMDGIEVGRVNVSARACGVGIRAFELAV
                     RYAQQRSTFGKPIAEHQAIAFQLAEMATKVEAGHLMMVNAARLKDSGERNDLAAGMAK
                     YLTSEYCAEVTQQSFRIHGGYGYSKEYEIERLMRDAPFLLIGEGTSEIQKQIISKRLL
                     AEYQI"
     gene            8359..9045
                     /locus_tag="VF874_08505"
     CDS             8359..9045
                     /locus_tag="VF874_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9176122.1"
                     /translation="MTVPDFVARPQLSQDVARYVRRRIFDGAYAAGEYVRLEQLAAEL
                     GVSVTPVREALLELRAEGLLVQQPHRGFMVLPVTGRDIADVSTVQAHIGGELAARAAA
                     NITDEQLTQLKDLQAQLEDAYKQHDRERAVRLNHEFHRAINLAADSPKLAQLMGQITR
                     YAPEAVFPTVEGWPGQSVRDHRRVIAALEKRDEERARTAMAEHFVAGVAPLVEHLMEQ
                     GVVNHADRSS"
     gene            9095..10327
                     /locus_tag="VF874_08510"
     CDS             9095..10327
                     /locus_tag="VF874_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9176123.1"
                     /translation="MSVRVIDEAANVFADPKAYTDEARLHAALTHLRANAPVSWVDVP
                     GYRPFWAITKYTDVMEIERANTLFTNWPRPVLAVALGEEASAGTVRTLIHMDDPQHRV
                     VRAISADWFRPKAMRALKVRVDELAKIYVDKMLTLGSECDFAQEVAVNYPLYVILTLL
                     GLPESDFPRMLKLTQELFGSEDDEFKRGSTTEEQLPALIDMFEYFKGVTASRRENPSE
                     DLASAIANARVDGELLSDIDTLSYYLIVATAGHDTTSATISGGLLALIENADQRERLR
                     DNLSLMPLATEEMIRWVTPVKTFMRTAAEDTTIRGVPIAAGESLLLSYVSANRDEDIF
                     ADPFRFDVGREPNRHVAFGYGVHFCLGAALARMEVNSFFTELLPRLKAVELTGAPQQM
                     ATTFVGGLKHLPIRYSLT"
     gene            complement(10391..11106)
                     /locus_tag="VF874_08515"
                     /pseudo
     CDS             complement(10391..11106)
                     /locus_tag="VF874_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245812.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
BASE COUNT         1947 a         3587 c         3722 g         1850 t
ORIGIN      
        1 gctgcaacgc ggctgccagg cgatccgcga gctgaagacg ctggaactca tcgaagagat
       61 cgcgagtcac ggcgcgtaag cgtgcgcctg ctacaaagcc gctacagtcg tagcatgtcg
      121 gaggtgtcct cacgcgatct aaacaacgac acggctggag tgctccgccg cgtcgaagcg
      181 ggagacgaca tcaccatcac agtcaaaggg cggcccgtcg cattcgcgca acgtctcgcc
      241 acgctcgaat ccgtggcaga catggaaagc ttgcccgtgg acgaaaaagc atcacgaaag
      301 ttgccggtga tcacgcagga cgatgatttg gacggcctcg acggtgcggc caacctgacg
      361 atcatccgag tctgaccgtt aggcggagtc acggcgcgtc gtagagagcg gcgacgtcgc
      421 cgggcctgac cttcatcgcg gcgttgcgtg gcagttcgtc gacaaccgtg atcgcgaccg
      481 ggacgtggtg gctgggcagc gactcgcgta cccagtccat gagttcggat tccgacgcca
      541 tctgcgatcc gggcctcact tcgactgcgg cgaagggcgc ttcgtcgaga cggcgatccg
      601 ggacgccgac gacgcacgcg tcgcttacgg cggggtggct gaccaacgcc tgccgtacgg
      661 tctccggcaa gaccttgaat ccgccgcgat tgatcgcccc gtccgccctg tcgtggatgg
      721 tgacgaaccc gtcctcgtcg actgcggcca gatcggtggt gcgaatccag tcgggcccga
      781 tcacgtcgat gcgtgcttcg ataagacccc tcccgcctgc gaccgcctcg gcccccgtct
      841 ccgggtcgac aatgcggacc tgcacaccgg gcaacggctt gcccaccgtg tcgcgtttgg
      901 tgccgccgta ctgccggtcg agggccggag tcagtgcaca cgccgagccg gcgaattcgg
      961 ttgcgccata ggcccacaac agcggaatac cgtagcgtcg ctcgaactcc tcccttactg
     1021 ccggcgcgag aggcgccgat cctccggcaa ggtagtccag cgatgccaag tcctctttag
     1081 gcacgtcggc gtcgagcagc atccgaatga tggctggttg caccccggcc tttgtgattc
     1141 cgtacgtctt cacggcacga acgaactcgt cgacggtgaa cttctccagc agcaccgttc
     1201 gcttaccgat gaacggcccg gtgagcagct ggcacacacc gatgctgccg aatggccagt
     1261 acaccagttc gggcgggtcg tccggcgatg tggttcggcc gacggtcatc gacagcacgg
     1321 tgtgttgcag cacttgggct ttgaccggta tgcgcttggg tggtccggtg gtgccgctag
     1381 tcaggatgtg aaggcccgcc gccgcgagag tcggatcgga tttgacgtcg tcacgcggtc
     1441 gtcgcgatgc cgccacctcg accccgtcca gcgacatcat cacgcccgca gcgcctgtcg
     1501 ccgtcacggc ttctgcgacc ggggccgtcc agtcgtcacg gtcgagcaga acggcaggca
     1561 gccgaagctg ttcgatatca cgggcgatcg actgcgggga ctggtacgag tagatcatcg
     1621 cgaccggccg tcgagcagcg atgaacccga ggatggccgc cgcatgcggc acgcggttgc
     1681 gcaccaccac ccccaccgga tcggtggggg gcactccggc tcgtgacaag gcggcggcga
     1741 tgtcctcgat gtactcggtg atctcgttgc ccgagtacca cttccgctcg aactcgatgc
     1801 acggccgatc gccgaagctg ttcagccgcg ccgccagagt ctcggcgaaa tcgggttcag
     1861 ccattggcgt cagtattgcg tcgagccttg gtcgacagag atccgggccg ccgtgacatt
     1921 cttcgattcg tcgctggcca gccacagcac cgcgtcggcg atgtcgtcgg gctgggcgac
     1981 ccagttcggc aggaacggcg tgagcatgtt cgccaactgg ggattggttt cggtggcctt
     2041 catgaccgtg tcgaccatct ctccgcttcc catcggggtg ttgaccggtc ccgggtgcac
     2101 gctgttgacc cggatcgagt atttacccag ctcggcggcg aaggcccggg ccatgccggt
     2161 caccgcgtgc ttgctcgcgg tgtagtgaac catgaacggc tgcaccttga ttcccgccgc
     2221 cgagctgatc aggatgatcg atccgccacg gccgccgtcg acgatcttct gcgcgcctgc
     2281 catcacggtg ttccaggtgc cggtcacgtt gacgtcgatg acgtcgcgga agtcctccgg
     2341 cgtgatgtcg ctccacacct gcggagcggc gatagcggcg ttcgccacga tgatgtcgag
     2401 ccgtcccagg gcggcgacgc cgtcgtcgac agctcgccga agtccgtcga aatcccttgt
     2461 gtcgacgaca gaaaagagaa tccgacggcc ggtgtcttcg acccgtcgga ccgttttggc
     2521 gacgtcgtcg ggagtggccg agcggtacgg aacgcatgac ggcagcttgc ccgccacgtc
     2581 cacggcgagg atgtccgcac cctcggcggc catccgcacg gcgtgcgcac gaccctgtcc
     2641 ccgggccgca ccggtgatga acgccacctt gcccgctagc cggccgctca cccccgctcc
     2701 cgctgcgcag ccttgacgag cccgccgccg atgatcaagc gctgaatttc gcttgtccct
     2761 tcgtacaacc gcaacagccg aacgtcgcgg tagatgcgtt ccactggcac gccctgcatg
     2821 tagccgctgc cgccgtgaat ctgaacggcg agatcggcta ccttgccggc catttcggtg
     2881 cagaacagct tcgccgccga cggcgcgatc ctgcggtcct cacctgcgag ccacagcttc
     2941 gcagcgtcgc ggaccagggc ccgtccggcc atcaccgcgg tctgctggtc ggcgatcatc
     3001 gcctgcacca gttggaactc tccgatcggt gtgccgccct gggtcgcggt cgcggcgtac
     3061 gccaccgact cgtcgagcgc tcgctgcgcc gcgccgacgg cgagcgctgc gatgtggata
     3121 cggccacgcg ccagggcggt catcgccgcg cgatatccga tgtcttcact gccgcccacc
     3181 aacgcgctgt cgggcacgcg cacgtcggtg aagctgacgt cggcggtcca ggcgccttcc
     3241 tggcccatct tggcgtcctt cgcgccgacc tcggcaccgt ccgcgtcggc cggcaccaag
     3301 aagacagcga tccccgcgcc ttcgtcgtcg gccgggcggg tgcgtgcgaa caccacgaac
     3361 agctgagcca ccggggcatt ggtgatgaac cgcttctgcc cggagatcac ccagttgccg
     3421 ccgtcacgga ttgccttggt gcgcaaccct gatgggttcg agcccgcgcc aggctctgtc
     3481 agcgcgaacg acgcgacgac ctcacccgac gccatgcctt ccagccagcg ggccttctgc
     3541 tcgtcggtgc cgaagccgac cagcacctgg cccgcgatgc cgttgttcgt gccgaacatc
     3601 gaccgcagcg caagcgaggt gtagccgagc tccatggcca gctcgatgtc ttgggccacg
     3661 ttcaggccga gaccgcccca ctcctgcgga atcgcgtacc cgaacagccc catcttcttc
     3721 gcccggtccc ggatgtcgtc cggaactcgg ttgtcggcca tgatttcctg ctcgcgcggc
     3781 accaccgcgg tgcggacgaa gtgacgcgtc tgggcgagga tgtcctggaa gtcctcgtcg
     3841 ctgacgactt cccgtgttgt agtcatctat cgaggctcct tctccgcgcg gcggggcggc
     3901 ggccgggcga aatcatatat gaaatatgat gtgcgccgac gggcgggtgt gcaccgacgc
     3961 gagggttccg cgacgatgag tgatgagagg tgacgggtgt cgatgctgaa cggtcagacc
     4021 gcggtcatca ctggtggcgc gcagggtcta ggtttcgcga tcgccgaacg atacgtcgcc
     4081 gaaggtgccc gagttgtcct cggcgacctg gacatcggcg ccaccgaggc cgcgaccgag
     4141 cggctcggcg gcgacaccat cgcggctgcc gtgaagtgtg acgtcaccca ggccgccgac
     4201 gtcgacaccc tggcatcaac cgctgtcgag aggttcggtg ggctggacat catggtgaac
     4261 aacgcgggca tcacttgcga cgcgaccatg cgaaagatga ccgaggagca gttcgatcag
     4321 gtcatcgcgg tgcacctcaa gggcacctgg aacggcatcc gcagcgcggc ggccatcatg
     4381 cgcgacaaca agcgcggcgc gatcgtgaac atgtcgtcgc tgtcgggaaa ggtcggtctg
     4441 gtcggccaga cgaactattc ggcagccaaa gccgggatca tcgggatgac gaaggccgcc
     4501 gcgaaggaac tcgcccacct cggcgtgcgg gtcaacgcga ttgcgccagg gctgatccgc
     4561 tcggcgatga cagaggctat gccgcaacac atttgggatc agaagatcgc cgaggtgccg
     4621 atgggccggg ccggcgaacc ggcggaggtg gccaaggtgg cgctgtttct ggccagcgac
     4681 ctgtcctcgt atatgaccgg caccgtgctg gaagtcaccg gcgggcggtt catgtgatga
     4741 ccatgcgcga agcagtgatc tgtgaacccg tgcggacgcc gatcggtcgc tacggcggaa
     4801 tgttcaagtc cctcaacgcc gttgacctcg gcgtcgcagc gctgacgggc ttgctcgaca
     4861 gaaccgggat cgcgccggac gcggtgcagg acgtcatcct ggggcattgc tatccgaaca
     4921 gcgaggcacc cgcgatcggg cgggtgatcg cgctcgacgc gggcctgccc gtgacggtgc
     4981 ccggcatgca ggtcgatcgc cgatgcggat cgggcctgca ggcggtgatc caggcgtgcc
     5041 tgcaggtggc caacggcgac aacgacctcg tcgtcgcggg cggcaccgaa agcatgagca
     5101 acgtcgcgtt ctattccatc gacatgcgat ggggaggcgc acgcacgggc atcagggtgc
     5161 acgatggcct cgcccgcggc cggaccaccg cgggcggccg cgactatccg gtgcccggcg
     5221 ggatgttgga gaccgccgag aatctgcggc gccagtacag catctcccgg caggagcagg
     5281 acgagttggc cgtgacatcg caccagcgcg cggtcgccgc ccagaaggac gggatcctcg
     5341 ccgacgagat cattccggtc gccgtacgaa cgcggcacgg cgaggaactg gtcgacaccg
     5401 acgagcatcc gcgcgccgat acctcggtcg agacgctgag caagctcaaa ccagttctac
     5461 gcgaagaaga ttcagaggct accgtcaccg ccggaaacag cagcggccag aacgacgccg
     5521 cgtcgatgtg tgtggtgacc acgccacaaa aagccgacga gctgggcctg acgccgttgg
     5581 tccggttggt gtcgtgggcc gtggccgcgg ttgcgccgag cgtcatgggc atcgggccgg
     5641 tgccggcgac cgaggccgcg ctttccaagg cggatctgcg cctggccgac gtcgacctca
     5701 tcgaactcaa cgaagcgttc gcggcgcagg cgctggcggt gatgcgcgag tggggactga
     5761 ccgccgccga ccgtgaccgc accaacgtgc acggctcggg gatctcgctg ggccatcccg
     5821 tcggtgcgac aggcgggcgg atgctggccg cgctggcccg tgagctgaac cggcgcgagg
     5881 ctcgatacgg gctggagacg atgtgcatcg gcggcggcca ggggctcgcc gcggtcttcg
     5941 agcggtggga ccagtgacct tgccgctgga cgggatcacc gtcgtcgagg tgtcgagctt
     6001 cgttgccgcg ccgctgtgcg ggttgacgct gtcgcaactt ggggccgagg tgatccgtgt
     6061 cgaccccatc ggcggcgcgg ctgacttccg ccggtggccg ctcgctgaga gcggcaggtc
     6121 gatctactgg accgggctga acaagggaaa gcggtcggcg acaatcgact tgcggtcgct
     6181 cgaggggcaa gagctggtgc agcggttgat cgccgagagt gaaggcatcg tggtgacgaa
     6241 cgccgcgggg ctggcctggt tgtcgcacga acgtctggcc gcgatgcggt cagacgtcat
     6301 ctccgtccaa ttgctcggac gcggagacgg ctccaccggc gtcgactaca ccgtcaacgc
     6361 agcgattgga ttcccctacg tcacggggcc agcggattac gcgggcccga tcaaccatgt
     6421 gttgccggcg tgggacgtct gctgcggctt gtacgctgca ctcgcggtga ccgcggcact
     6481 gcggcggcgt gaccagactg gggacggcac ccacgtccgc ctggcgctgg aggatgtcgc
     6541 gctggccacc gtcggcaacc tcggcttgat caccgaacct caggtcaacg gcacgcagcg
     6601 accccgcctt ggcaacgcga tcttcgggca gtacggccag gatttcacca gccgtgacgg
     6661 cgcgtcgttc atggctgtgc tgttgacagg caggcacttt cgcgacctgg tcgaggtgac
     6721 gggcaccgga gcagcggtgt cggccctcgc cgacgctctg ggcaccgact tcagctccga
     6781 aggtgaccgc taccgctacc gcgacgtgct gtccgggttg ttttcgacgt ggttcgccga
     6841 gcattcggcc gccgagatca atgacgcgct gtcgtccacc accgtgctgt tcgagcggta
     6901 tcggacgttc gcggaggtgg ccaccgacga tcgggtggtg gctaatccat tgttcacacg
     6961 gatgcatcag cccggcgtcg gagagtattt cgcgccgggg atgccggcgt cgttcgacgg
     7021 aagccatccc gcgagcgcgc ccgccccgaa cctggggcag gacacggccg ggctgctcgc
     7081 cgaacggctc ggactgagtg ccgacgagat cacccgcctc gagaacgcgg gaacaatcgc
     7141 ctaacgcagg gcagaaggga agccacacat gaccagactc gctcagacgc tcggccttac
     7201 cgagttccaa actgagatcg tcttcaccgt aaggcaattc gtcgaaaagg aagtcatccc
     7261 gaacgcgccg gaactcgagc gcacggacac ctatccgcag gacatcgtcg accagatgaa
     7321 ggacatgggc ctgttcgggc tgatgattcc ggaggagtac ggcggactgg gggagtcgct
     7381 gctgacgtac gcgttgtgcg tcgaggagct ggcccgcggc tggatgagcg tctcgggggt
     7441 gatcaacacc catttcatcg tggcgtacat gctgcgccag cacggcaccg atgcgcagaa
     7501 gcagcaccat cttccgcgga tggcgaccgg tgaggcccgc ggtgcgttct cgatgtcgga
     7561 gccggagctg gggtccgatg tcgccgcgat ccgcacgcgg gccaagcgca atgccgacgg
     7621 caactacacc atcgacggtc agaagatgtg gttgaccaat ggcggcagtt cgacgttggt
     7681 cgcggtcttg gtgcgtaccg acgaaggcgc ggacaagccg caccgcaact tgaccgcatt
     7741 cctcgtcgaa aagccggtcg gcttcggcga agtcgcgccg ggcctggtga ttcccggcaa
     7801 gatcgacaag ctcggctaca agggaatcga caccacggag ttgatcttcg acggctatgc
     7861 cgccagcgcc gacgacatcc tcggtggcgc accggggcag ggtttcttcc agatgatgga
     7921 tggcatcgaa gtcgggcggg tcaacgtctc tgcccgggcc tgcggggtgg gtattcgcgc
     7981 attcgagctg gcggtccggt atgcgcagca gcgttcgacg ttcggcaagc ccatcgccga
     8041 gcatcaggcg atcgcgttcc agctggccga gatggcgacg aaagtcgaag cgggacatct
     8101 gatgatggtg aacgccgcgc ggctgaagga ttcaggcgag cgcaacgact tggccgccgg
     8161 catggccaag tacctgacga gcgaatactg cgcggaggtc acccagcaga gcttccgaat
     8221 ccacggcgga tacggctact ccaaggaata cgagatcgag cggctgatgc gcgacgcgcc
     8281 gttcctgctc atcggcgagg gcaccagcga gattcagaag cagatcatca gtaagcggtt
     8341 gctggccgag taccagatat gacggttccg gatttcgttg cgcggccgca actgtcgcaa
     8401 gacgttgcgc gatatgtccg tcggcggatc ttcgacggcg cgtacgcggc aggggaatac
     8461 gtccgtctgg aacagctggc cgcggagttg ggggttagcg tgacaccggt gcgtgaagcg
     8521 ctgctcgagt tgcgcgccga ggggctgctc gtccaacagc cgcaccgcgg cttcatggtg
     8581 ctgccggtga cgggaagaga catcgcggac gtgtcaactg tccaggcgca catcggaggg
     8641 gagttggcgg ctcgggcggc ggccaacatc accgacgagc agctgacaca actgaaggac
     8701 ctgcaggcac agctcgagga cgcctacaaa caacacgacc gcgagcgtgc ggtgcggctg
     8761 aaccacgagt tccatcgtgc gatcaacctg gcggccgatt caccgaagtt ggcgcagctg
     8821 atgggccaga tcacccgcta tgcacccgag gcggttttcc cgaccgtcga aggttggccg
     8881 ggccagtccg tcagggatca ccgccgcgtg atcgccgcgc tggagaagcg cgacgaagaa
     8941 cgcgcccgga ccgcgatggc cgagcacttc gtcgcggggg tggcgccgct ggtcgagcac
     9001 ctgatggagc aaggcgtcgt caaccacgcc gaccggtcgt cttgatcacg cacctgcacc
     9061 tcactactgt aagtgcaaca gtgaggggtt gcttatgagc gtgcgggtga tcgacgaggc
     9121 agccaacgtg ttcgcggacc ccaaggcata caccgacgaa gcgcggctcc atgccgcact
     9181 gactcatctc cgtgccaatg ccccggtgtc gtgggtcgac gtaccgggtt accggccgtt
     9241 ctgggcgatc accaagtaca ccgacgtcat ggagatcgag cgggccaaca cgcttttcac
     9301 caactggccg cgcccggtgc tggcagttgc gttgggcgag gaggcgtccg ccggtacggt
     9361 gcgcacgctg atccacatgg acgacccgca gcaccgagtg gtgcgcgcga tcagcgcgga
     9421 ctggttccgc cccaaggcca tgcgcgcgct gaaggtgcgc gtcgacgaac tcgccaagat
     9481 ctacgtggac aagatgctga ccctcggaag cgaatgcgac ttcgcccagg aggtcgcggt
     9541 caactacccg ctctacgtca tcctgacgct gctcggtcta ccggaatcgg attttccgcg
     9601 gatgctgaag ctcacccagg agttgttcgg cagcgaggac gacgagttca agcggggcag
     9661 cacaaccgag gagcagttgc ctgccctgat cgacatgttc gagtacttca aaggggtgac
     9721 cgcgtcgcgc cgcgagaacc cgagcgagga cctggcgtcg gcgatcgcca acgcccgcgt
     9781 cgacggtgag ctgttgtccg atatcgacac cctgtcctac tacctcatcg tcgcgaccgc
     9841 cggacatgac accaccagcg cgacgatctc gggcgggtta ttggccttga tcgagaacgc
     9901 cgaccagcga gaacggttgc gtgacaacct atcgctgatg ccactcgcga ccgaggagat
     9961 gatccgctgg gtgactccgg tcaagacatt catgcgtact gcggccgagg acacgacgat
    10021 tcgcggcgtg ccgatcgcgg cgggggaatc actgctcttg tcttacgttt cggcgaatcg
    10081 tgacgaggac attttcgccg accccttccg gttcgatgta ggccgcgagc cgaacaggca
    10141 cgtcgccttc ggttacggtg tgcacttctg tctgggcgcc gcgctcgcgc ggatggaggt
    10201 gaacagcttc ttcaccgaat tgttgccgcg gctgaaggcg gttgaactga ccggggcacc
    10261 acaacagatg gcgacgacgt tcgtcggcgg actgaagcac ctgccgatcc ggtactcact
    10321 gacctgaaat gtgcgttgac tctgcgtcct gggtgcagaa gtgcgagaag ctcgcgccct
    10381 cggcgcagaa tcaacgtaac aggtcgcgca ggcggtggac gtcgaccttc ccggtgggcc
    10441 cgcgggggat ggcgtcatcg gagtccacca ggagccacac agtaggaacc ttgaacgagc
    10501 tcaacagctt tcgagcggaa tcacgcagct gctcgcgcgt catcatcgcg gcctcacaca
    10561 ccaccgccgc accgacctca ccgtcgacat tggtcacgaa cgcgttctcg acgccgtcga
    10621 tggtccgcag cgcctgctcg acctcgctcg ggtagacggt ggcgccacgg accttgaaca
    10681 tgtcgtcgga tctgccgtgg tagaacagga agccgtcgtc gtcgagacgg ccaaggtcgc
    10741 cggtgggata gaacccgtcg gcggtgaaca gatcctcgcg gctgcggcga cacacccctc
    10801 gcaaggtgtg cggcccccgg atctggatca tgccgacggt ccccgccggg acggacatgc
    10861 cgctgtcggg atcgacgatc cggacttcca tgccgtcgaa cggctttccg caactgcccc
    10921 aagcggatcg cggcatgtcg gtgtcggcgg gatagccgca ttagggtccg aaggcctccg
    10981 tcatgccgaa cagcttcgcc cgggcccccg gctgggcgcg atggtccggc ggcagcaggg
    11041 cctccaagct tcccggccgc agcgatgaca ggtcggcgcc caccgaatca gctcggcgcg
    11101 ctagcg
//
LOCUS       DASWBF010000235        10660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_606034,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000235 DASWBF010000000
VERSION     DASWBF010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606034"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="VF874_08520"
     CDS             complement(<1..97)
                     /locus_tag="VF874_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9176124.1"
                     /translation="MRCAVTGATGYVGGRLVPQLLDRGHDVRAVAR"
     gene            163..900
                     /locus_tag="VF874_08525"
     CDS             163..900
                     /locus_tag="VF874_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9176125.1"
                     /translation="MTDVKSTLPPALRKALELLADPPANPDASKGYLDLLGDDGAVPK
                     NTGAIQAVWASPIGSMFYDNAQALARRFFSAWQHPTDWLNIPQGGTALDVGCGPGNVT
                     ASLGRAVGQGGLALGVDISKPMLARAVRAYSGPQVGFLRADAQRLPLRDDLIDALVSI
                     AVLQLVPDPAAAVAEMARVLRPGGRLAVMVPTVGRPGPPSRFFPNAGAHFFGDDDLGD
                     ILEDLGFVSVRTKNAGPLQWVRGRLAS"
     gene            complement(893..1318)
                     /locus_tag="VF874_08530"
     CDS             complement(893..1318)
                     /locus_tag="VF874_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9176126.1"
                     /translation="MWIRGVLFTAILAVAMTLPAIASATAADGVSATVLWQRAADGTD
                     YVFREITIAPGGSTGWHWHDGRLYGVIKEGTLTHNMADCSVDGVYPAGSGVFEPSGPD
                     RVHIGRNVGDTPLVMQILYILPAGSALSEDAPNPGCAFN"
     gene            1370..2455
                     /gene="purM"
                     /locus_tag="VF874_08535"
     CDS             1370..2455
                     /gene="purM"
                     /locus_tag="VF874_08535"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX9176127.1"
                     /translation="MSGRAKDRVISAGTSYASSGVDIEAGDRAVELLKPLARKATRPE
                     VVGGLGGFAGLFALKGGYREPLLAASTDGVGTKVAIAQAMDKHDTVGLDLVAMVVDDL
                     VVCGAEPLLLQDYIAVGRIVPERIAALGAGIAEGCVQAGCALLGGETAEHPGLMAPDA
                     YDISATAVGVVEADDVLGPDRVRPGDVAIGMASTGLHSNGYSLARHVLLEIDRMNLAG
                     HVEEFGRTLGEELLEPTRIYAKDCLALAAETQVKTFCHVTGGGLAGNLARVVPHGLQA
                     ELDRGTWTPAPVFKMIAQRGRIDRAEMEKTFNMGVGMVAVVSPDDTDRALAILTARHL
                     DCWVLGTVKKGGKSSSRAELVGRHPRF"
     gene            complement(2480..2647)
                     /locus_tag="VF874_08540"
     CDS             complement(2480..2647)
                     /locus_tag="VF874_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HEX9176128.1"
                     /translation="MGRGRAKAKQTKVARELKYSSPQTDFERLQRELSGAEHVDADTT
                     DPWDDEDNWRR"
     gene            complement(2854..3975)
                     /locus_tag="VF874_08545"
     CDS             complement(2854..3975)
                     /locus_tag="VF874_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEX9176129.1"
                     /translation="MSAVPAPAASPDAGAIWHYGDPLGEQRAALTDAVYIDRSHRGVI
                     TLTGPDRLSWLHSISSQHVADLSDGTTVENLSLDGQGRVEDHWIQTELAGVSYLDTEP
                     WRTEPLLAYLKRMVFRAEVVPDAADYGVLSLLGPKLADPDVLKTLGIDSPPGELVAVP
                     LAGGGFVRRMPTQRDGPAVALDLVVPRDQLPQWHDRLQKAGLRPAGVWTYEAHRVAGL
                     RPRLGVDTDERTIPHEVGWIGTAVHLDKGCYRGQETVARVHNLGRPPRMLVLTHLDGS
                     VSRPITGAPITAGGRAVGRLGTVVDHVDLGPVALALVKRGLAADTPLTVNADDAGDER
                     SREEQKTEVALTIDPDTVPVAGGVGAGRVAVDRLRGVTR"
     gene            4013..4864
                     /locus_tag="VF874_08550"
     CDS             4013..4864
                     /locus_tag="VF874_08550"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEX9176130.1"
                     /translation="MSVLVTLDGRFHDPEAPLLHADDLAAVRGDGIFETLLVRDGRPC
                     LLEAHLQRLAQSADLMDLPAPDADAWRSAAAGAADRWVAESSDEGVMRWVYSRGRESG
                     GPPTAYITVGRLPERVAAARNDGLAAVTLAREMPTTASGAPWLLAGAKTLSYAVNMAA
                     LRYAAERGAGDVIFVSTDGYVLEGPRSTVVIATSDGLQTPPPTLPILHGTTQQASFTV
                     AAEKGYRCEYRPLRPADLLAAQGIWLVSSITLAARVHTLDGRTLPPAPQASELAELVD
                     LAIVSDR"
     gene            complement(5147..5449)
                     /locus_tag="VF874_08555"
     CDS             complement(5147..5449)
                     /locus_tag="VF874_08555"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9176131.1"
                     /translation="MKHTIAGAAAAGLAAIALATSPIAAAGPEEDFLIAVAAAGISSG
                     SPDQIVALGRSVCTDQAAGTAFNQVVSNLAASRGWSPGQASAFVGAATAAFCPQLT"
     gene            complement(5555..6205)
                     /locus_tag="VF874_08560"
     CDS             complement(5555..6205)
                     /locus_tag="VF874_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024636948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEX9176132.1"
                     /translation="MNSPSAQPGSGDRAVAAAAERAKTTASRNIPVFEDLPLPADTAN
                     LRKGANLHDALLALLPLVGVWRGEGEGRGPHGDYRFGQQIIVSHDGRDYLNWEARSWR
                     LTEKGEYDEAALRETGFWRFVSDPADPAEMQAIELLLAHSAGYVELFYGRPLNQSSWE
                     LVTDALARSRSGVLVGGAKRLYGIVEGGDLAYVEERVDADGGLVPHLSARLSRYAG"
     gene            complement(6365..6667)
                     /locus_tag="VF874_08565"
     CDS             complement(6365..6667)
                     /locus_tag="VF874_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1416 domain-containing protein"
                     /protein_id="HEX9176133.1"
                     /translation="MCSAPKQTQTLPASVDVEKETVITGRVIDGSGQPVGGAFVRLLD
                     SSDEFTAEVVASATGDFRFFAAPGSWTVRALSSAGSGDAVVAPNGAGIHEVDVKVA"
     gene            complement(6670..7503)
                     /locus_tag="VF874_08570"
     CDS             complement(6670..7503)
                     /locus_tag="VF874_08570"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX9176134.1"
                     /translation="MARSDVLVTTDWAESNLDAPDTVFVEVDEDTSAYDTGHIAGAVR
                     LDWRKDLQDPVKRDFVDAEQFSKLLSERGISNDDTVILYGGNNNWFAAYAYWYFKLYG
                     HENVKLLDGGRKKWELDGRPLSTDTVEREPTSYTAKEPDNSIRAFRDEVLAAIGDKNL
                     VDVRSPDEFSGKILAPAHLPQEQSQRAGHIPGAINVPWSKAANEDGTFKSDEDLTKLY
                     GEAGLDESMDTIAYCRIGERSSHTWFVLRELLGHGNVKNYDGSWTEYGSLVGVPIELG
                     S"
     gene            complement(7517..7975)
                     /locus_tag="VF874_08575"
     CDS             complement(7517..7975)
                     /locus_tag="VF874_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEX9176135.1"
                     /translation="MSTTSREVDVRGPRFAAWVTSAVLVVTLLVSAVSPPAAAVVLGL
                     QAAVFAVSALRGPRYSPYGLIFAHLIAPRLGPVTEREPVAPLKFAQLVGFVFAVIGVV
                     GFAVGVTVLGEIATAFALVAAFLNAAFAICLGCQLYPLVARLKHAPLTVR"
     gene            complement(8159..8578)
                     /locus_tag="VF874_08580"
     CDS             complement(8159..8578)
                     /locus_tag="VF874_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9176136.1"
                     /translation="MSSSLVLAGCVIAAALGVAAVIGVLVNRRSGVLRDSMQVVDHGA
                     DLGLSRTGPTVVHFSAVWCGPCAAVRRVVNQVCGDLPAVAHVEIDMDANPAVARRLSV
                     LSLPTTFIFDADGRQRYRTAGVPKAADLRSKLVPLLA"
     gene            complement(8575..9372)
                     /gene="lmeA"
                     /locus_tag="VF874_08585"
     CDS             complement(8575..9372)
                     /gene="lmeA"
                     /locus_tag="VF874_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannan chain length control protein LmeA"
                     /protein_id="HEX9176137.1"
                     /translation="MRALLIGITVMFVALAGGAVGADFGTAIYAEYRLASTLRAEAGL
                     GFDPWVGILGFPFIPQALRHRYDEVEIKASGVDRPIVGSASLEATMHEVDLTQASWLI
                     RPGAPLPVGKLESRIIIDSTHLGRFMGINDLVVEAPPKETNDATGGTTESGISSSVGL
                     VFTGTPKAAGLQERVSVSVDLSMTGPGDTTLAFTATGILTGPGTADRTVPDEKLDAVL
                     KAFSKNLPGQKLPFGIAPNREGARGSDVIIEGIVEGLTVHLDEFTVS"
     gene            9532..10338
                     /locus_tag="VF874_08590"
     CDS             9532..10338
                     /locus_tag="VF874_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9176138.1"
                     /translation="MDLLLLTGDPHPEAVLPSLSLLPHTVRRAPSEVSSLLDAGSADV
                     AIVDARTDLAAARGLCRLLGTTGTLVPVVAVVNEGGLVAINVDWGLDDILLPGTGPAE
                     IDARLRLLVGRRGGAANQENVGKITLGELVIDEGTYTARLRGRPLDLTYKEFELLKYL
                     AQHAGRVFTRAQLLQEVWGYDFFGGTRTVDVHVRRLRAKLGTEYESLIGTVRNVGYKA
                     VRPARGRPAADEELDDDSEAEFAPAANELPQSVSDALRASGPAPDPLRTP"
     gene            10335..>10660
                     /locus_tag="VF874_08595"
     CDS             10335..>10660
                     /locus_tag="VF874_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9176139.1"
                     /translation="MTDIDWRSSLAPGDEHQVRELIAAAARADGVAPVGEQVVRELPL
                     QRTKHLVAADGQRVVGYLNLVPETETAPPIAELVVAPDVRRRGVGASMVRAAIDAGGD
                     ATRIWA"
BASE COUNT         1717 a         3565 c         3666 g         1712 t
ORIGIN      
        1 tcctggcgac cgcccgcacg tcgtggcctc tgtcgaggag ctgtggcacc agccgtccgc
       61 cgacgtaccc ggtcgcgccc gtcaccgcgc agcgcatctg tttcctcccg tgtcggcgcc
      121 cttcgtatgc tattcggagc catgccactg caggatggac gcatgaccga tgtgaagtcc
      181 acgttgccgc ccgcgttgcg caaggcgctg gagctactcg ccgatccgcc cgcgaacccg
      241 gacgccagca aggggtacct cgacctgctg ggcgacgacg gcgccgttcc gaagaacacc
      301 ggcgcgatcc aggcggtatg ggcgtcgccg atcgggtcga tgttctacga caacgcccaa
      361 gccttggccc gcaggttctt cagcgcctgg cagcacccca ccgactggct gaacattccg
      421 cagggtggga ccgcactcga cgtcggctgc ggccccggca atgtcaccgc gtcgctgggc
      481 cgcgccgtcg gccagggcgg tctggcgttg ggcgtcgaca tatcgaagcc gatgctggcc
      541 cgcgcggtgc gcgcttactc ggggcctcag gtcgggttcc tgcgcgccga cgcgcagcgg
      601 cttccgctgc gcgatgacct tatcgacgcg ctcgtgtcga tcgccgtgtt gcagctggtt
      661 ccggacccgg ccgcggcggt cgcagagatg gcgagggtct tgcgaccggg cgggcgactg
      721 gccgtgatgg tgcccaccgt cgggcgtccg ggcccgccgt cgcggttctt tccgaacgcg
      781 ggtgcccact tcttcggtga cgacgacctc ggcgacatcc tcgaggacct cggattcgtc
      841 agcgtgcgga cgaagaatgc cggtcctctt caatgggtgc ggggaagact cgctagttga
      901 aagcgcatcc gggattgggc gcgtcctcag acaacgcgct gccggcgggc aggatgtaca
      961 gaatttgcat caccagcggg gtgtcgccga cgttgcggcc gatgtgcacg cgatcgggtc
     1021 cgctcggttc gaacacgcct gagccggccg ggtagacgcc gtcgacggag cagtcggcca
     1081 tgttgtgcgt cagcgtgccc tctttgatca cgccgtacag ccgcccgtcg tgccaatgcc
     1141 atcctgtgct gccgcccggc gcgatggtga tctcgcggaa cacatagtcc gtgccgtcgg
     1201 ccgcgcgctg ccacagcacg gtcgcgctga caccgtcggc cgcggtggcc gaagctattg
     1261 cgggcaaggt catcgcgacg gcgaggatcg cggtgaacaa gacaccgcga atccacatac
     1321 cggtaccctt gcacgcgatg agcgagtgcg ccgagcgaat caagaaccgg tgagcggccg
     1381 ggccaaagac cgggtcatct cggccggcac ctcatacgcg tcctccgggg tggacatcga
     1441 ggcgggcgat cgagcggtcg aactactgaa acccttggcg cggaaggcca cgaggccgga
     1501 agtcgttggg ggccttggtg gattcgcggg actgttcgcg ctgaagggcg gctaccgcga
     1561 gccgctacta gccgcgtcca ccgacggtgt gggaaccaag gtcgcgatcg cccaagccat
     1621 ggacaagcac gacaccgtag gactggatct ggtggccatg gtcgtcgacg acctcgtggt
     1681 ctgcggcgcg gaacccctgc tgctgcagga ctacatcgcc gtcgggcgca tcgtgccgga
     1741 gcggatcgcg gcgctgggtg cgggcattgc agagggctgt gtgcaggcgg gatgcgcgct
     1801 gctcggcggc gaaaccgccg agcatccggg actgatggca ccagacgcct acgacatctc
     1861 tgccaccgcc gtcggcgtgg tggaggccga cgacgtgctc ggtccggatc gggtgcgtcc
     1921 cggcgacgtg gcgatcggga tggcgtcgac gggtctgcac tccaacggct actcgctggc
     1981 gcgccacgtg ctgctggaga tcgaccggat gaacctggcg ggccatgtcg aggagttcgg
     2041 tcgcaccctc ggcgaggaac tgctggagcc gacgcggatc tatgccaagg actgcctggc
     2101 gctggccgcc gaaacgcagg tgaagacgtt ctgccacgtg accggcggcg gcttggcagg
     2161 caatcttgcg cgtgttgttc cgcacggtct gcaggccgaa ctcgaccgcg gcacctggac
     2221 gcccgcgccg gttttcaaga tgatcgctca gcgcggccgc atcgaccggg cggagatgga
     2281 gaagacgttc aacatgggcg tcgggatggt cgccgtcgtc agccccgacg acaccgaccg
     2341 cgcgctggcg attctcacgg ccagacacct ggattgctgg gtgctgggca cagtcaagaa
     2401 aggcgggaaa agcagcagcc gggccgaact cgtcggacgg cacccccggt tctgacgcga
     2461 acgcgtcggc gaggagcgat cagcggcgcc agttgtcttc gtcgtcccac ggatcggtgg
     2521 tatcggcgtc tacatgctcg gcgccggaca gctcgcgttg gagccgctcg aaatcagtct
     2581 gcggagagct gtatttcagc tcccgggcaa ccttcgtttg tttcgccttc gcccggccgc
     2641 ggcccatggg ggaaccccct cgcgcaataa cggagcggcc cgatgtgaag gcggctccga
     2701 tctgagtgtg tgtttgttag tcctggcaac actttaccgt gcccgcgcgc cgtccgctgg
     2761 cagggcttgg ctgtctcctc ttcgcccgag aggctcatcg gcgctgtctc ctcttcgccc
     2821 gagaggctca tcggcccgct ggtgcggcgc aggtcaccgc gtgacgccgc gcagccggtc
     2881 gaccgcgacg cggccagcgc cgacgccgcc cgcgactggc acagtgtccg ggtcgatcgt
     2941 cagcgcgacc tcggtcttct gctcttcgcg cgagcgctca tcgccggcgt cgtccgcgtt
     3001 gacggtcaac ggagtgtcgg cggcgagccc gcgcttgacg agtgccaacg cgacgggtcc
     3061 gaggtcgacg tggtccacga ccgttccgag ccgtcccacc gcgcgaccgc ccgcggtgat
     3121 cggcgcgccg gtgatcggac gcgacaccga gccgtcgagg tgggtgagta ccagcattcg
     3181 cggcggccgc ccgaggttgt gcacccgagc gacagtctcc tggccgcgat agcagccctt
     3241 gtccaggtgg acagcggtgc ctatccagcc gacctcatgc gggatcgtcc gctcatcggt
     3301 atcgacgccc agccgcggcc gcaggcccgc gacccgatgt gcctcgtagg tccagacgcc
     3361 ggccggccgc agcccggcct tctgtaaccg gtcgtgccac tggggcagct ggtcacgcgg
     3421 caccaccaga tccagcgcga cggcggggcc gtcgcgctgg gtgggcatgc ggcgcacgaa
     3481 cccgccgcct gccagcggca cagcgacaag ctcgcccggc ggcgagtcga tgcccagcgt
     3541 cttgagcacg tccggatcgg ccagcttcgg tccgagcagt gacagcacgc cgtagtcggc
     3601 cgcatcaggg acgacctcgg cccggaacac catccgcttg aggtaggcca gcagtggctc
     3661 cgtgcgccat ggttcggtgt cgaggtaact caccccggcc agttcagttt gaatccagtg
     3721 gtcttcgaca cggccctgcc cgtcgaggct gaggttctcg accgtcgtgc cgtctgatag
     3781 gtcggcgacg tgttgcgacg aaatactgtg cagccacgac agccgatcag ggccggtcag
     3841 cgtgatgaca ccacggtgtg accggtcgat gtacacggcg tcggtgaggg cggcgcgctg
     3901 ttcaccgagc ggatcgccgt agtgccaaat agcgccggca tcagggctgg cggcgggggc
     3961 gggcacggcg ctcacgcatt caactctacg attcaccgtt acggtgttgg ccatgtcggt
     4021 tctcgtcacc ctggacgggc gattccacga ccctgaggcg ccgctgctgc acgccgacga
     4081 cctcgccgcc gtgcggggcg acggcatctt cgagacattg ctggtgcgcg acgggcggcc
     4141 ctgcctgctg gaggcgcatc tccagcggct ggcgcagtcg gcggacctga tggacctacc
     4201 cgcgccggat gccgacgcgt ggcgatccgc ggcggcaggg gccgcggatc gctgggttgc
     4261 ggagagcagc gacgagggcg tgatgcgctg ggtgtacagc cgcgggcgcg agagcggcgg
     4321 cccgcccacc gcgtacatca ccgtcggacg gctgcccgaa cgggtcgccg cggcgcgcaa
     4381 cgatgggctg gccgccgtga cattggcgcg cgagatgccg accaccgcgt ccggcgcgcc
     4441 gtggctgttg gcgggcgcca agacgctgtc gtatgcggtg aacatggcgg cgctgcggta
     4501 tgccgccgaa cgtggcgcgg gtgacgtgat cttcgtcagc acggacggct acgtgctcga
     4561 gggcccacgt tcgacggtgg tgatcgccac ctcggacggg ctgcagacgc caccgccaac
     4621 gctgccgatt ctgcacggca ccacgcaaca ggcgtcgttc accgttgctg ccgagaaggg
     4681 ctaccgttgc gagtaccggc cgctgcggcc agcggatttg cttgctgcgc aaggtatctg
     4741 gctagtctcc agcatcacgc tggccgcccg cgtgcacacc ctggacggcc gcaccctgcc
     4801 accggcgccc caggcgtctg aactcgccga gctcgtcgac cttgcgatcg tcagcgaccg
     4861 ctgacgtcga acggagccta ctgcgaagat cctgcggaag tgattaattg cttgtcgcgc
     4921 caggggatcc caggtaactt cgccggtaca cgagaaggga ggtggtccga gaaattgatt
     4981 gacttatgga cgcgtgaggt gactgccggc tagcagccgc aagcgaggaa aaccccccgc
     5041 tgcaaggcgc tggtaattcc ccgcagtcac ccggcccccg agcccctggt ctgtccaacc
     5101 gggaacatcg gctcgggggc cgcttacttc ccgggagttg aaccgtttac gtcagctgtg
     5161 ggcagaacgc ggccgtcgca gcgccgacaa aggcgctggc ctgcccgggg gaccagcctc
     5221 tcgacgccgc gagattcgaa accacctggt tgaaggcagt cccggcggct tggtcggtgc
     5281 agacggacct cccgagggca acgatttgat cggggctccc tgaagaaatg cctgcggcag
     5341 cgaccgcgat gaggaaatct tcctcgggcc cggcggccgc gataggtgac gtcgccaatg
     5401 cgatggcggc gagtccggcg gcagcggcgc cggcaattgt gtgtttcatc gtcggtcctt
     5461 cctcgagtgg actcggcaac gccgagaatg acctcaattt ttgtcccgcc gaccgatgaa
     5521 ttcgcaggta ttcgcgaaat agcgcaggtt gttatcagcc cgcgtaccgg gacagccgcg
     5581 ccgacagatg cggcaccaag ccgccatcgg cgtcgacgcg ttcctcgacg tacgccagat
     5641 cgccgccctc gacgatcccg tacaaccgct tcgcgccgcc gaccagcact ccggatcgac
     5701 tgcgcgccaa cgcatccgtg accagttccc acgacgactg gttgagcggg cgaccgtaga
     5761 agagttcgac gtagccggcg gagtgggcga gcagaagctc gatcgcctgc atctcggccg
     5821 gatctgcggg gtcgctgacg aaccgccaga acccggtctc tcgcagggcg gcttcgtcgt
     5881 attcgccctt ctcggtgagc cgccaagagc gcgcctccca gttcaggtag tcccgcccgt
     5941 cgtgggagac gatgatctgc tggccgaagc ggtagtcgcc atgcggcccg cggccttcac
     6001 cttcgccgcg ccagacgccg acgagcggca gcagagccag cagcgcgtcg tgcaggtttg
     6061 cgcccttgcg caggttcgcc gtgtcggcgg gcaacgggag gtcctcgaag acggggatgt
     6121 tgcgggacgc cgtggtcttg gcgcgttcag ccgccgcggc caccgcccgg tctccggatc
     6181 ccggttgcgc gctgggtgaa ttcacgagtc gtcggaaatg agccggtaca ccgcgtacag
     6241 cgcgaaccac gcgattacca cggtgcacgc caccagcagg atctcgtaga acagcaccac
     6301 gagatcgagt ttaatgccga ccggccggcc gagcgctctc gcgccgtgcc gagctcaaaa
     6361 cgggctacgc gaccttgacg tcgacctcgt ggatgcccgc gccgttcggg gcgacgacgg
     6421 cgtcgccgct gcccgccgac gagagcgccc gcacagtcca cgacccgggt gccgcgaaga
     6481 accggaagtc accggtggcc gatgcgacga cctcggcggt gaactcgtcg gaggagtcca
     6541 gcagacgcac gaacgcgccg cccaccggct gcccggagcc gtcgatcacc cggccggtga
     6601 tcaccgtctc cttctcgacg tcgacactgg ccggcagcgt ctgtgtttgt ttcggtgcag
     6661 agcacataat cagcttccca actcgatcgg gacccccacc agggagccgt attcggtcca
     6721 acttccgtcg tagttcttga cattcccgtg cccgaggagc tcgcgcagca cgaaccaggt
     6781 gtgcgacgac cgttccccga ttcggcagta cgcaatcgtg tccatggact cgtcgaggcc
     6841 tgcctcgccg tagagcttcg tcaggtcctc gtcagacttg aaggtgccgt cctcgttggc
     6901 ggccttgctc cacggcacgt tgatggcgcc cgggatgtgc ccggcacgct ggctctgctc
     6961 ctgcggcaag tgggcgggtg ccaggatctt gccggagaac tcgtcggggg agcggacgtc
     7021 gaccaggttc ttgtcaccga tcgcagccag cacctcgtcg cggaacgcgc ggatgctgtt
     7081 gtccggctcc ttggcggtgt agctggtggg ctcgcgttcc accgtgtcgg tggacagcgg
     7141 gcgcccgtcg agctcccact tcttgcgacc gccgtcgagc agcttgacgt tctcgtgccc
     7201 gtacagcttg aagtaccaat aggcgtacgc cgcgaaccag ttgttgttgc cgccgtagag
     7261 gatcacggtg tcgtcgttgg agatgccgcg ctcggacagc agcttcgaaa actgttcggc
     7321 gtcaacgaaa tcgcgcttga ccgggtcctg gaggtctttg cgccagtcca gcctgaccgc
     7381 gccggcgatg tgcccggtgt cgtatgcgct ggtgtcctcg tcgacttcga cgaagacggt
     7441 gtcgggagcg tcgagattgc tctccgccca gtcagtggtg accaggacgt cggagcgagc
     7501 catgaatttc ctttccttac ctgacagtta gtggagcgtg cttcagccgc gcgaccagcg
     7561 ggtacaactg gcaacccagg cagatagcga atgccgcgtt gaggaacgcg gcgacgagtg
     7621 cgaacgcggt ggcgatctcg ccgagcactg tcaccccgac ggcgaacccg accacgccga
     7681 tcaccgcgaa gacgaacccg accagctggg cgaacttcaa cggcgcgacc ggttcgcgtt
     7741 cggtgacggg accgagacgg ggggcgatca ggtgggcgaa gatcagcccg taggggctgt
     7801 accgcggccc gcgcagtgcg gagaccgcga acaccgcggc ctgcaggccg agtaccaccg
     7861 ccgcggcggg tgggctgacc gccgacacca acagcgtcac gaccagcacc gcgctggtga
     7921 cccaggcggc gaaccgggga ccgcgaacgt ctacttcacg gctggtggtc gacatacaga
     7981 tactcctgtg ctggaaagag ctggcggaaa gggcacggct caggccgctg gcgaggcgct
     8041 cagcgacaac agcaacagaa acaacccgcg gtgcggcaca gatcgactgc gcggcgcttg
     8101 gtgagcatca gctcggggcg ggtagacacg tcggcaagct tacctaatgg cggtggcgtc
     8161 aagccaacag cggtaccagc ttggagcgca ggtcagcggc cttgggtacg cccgcggtgc
     8221 ggtaccgctg ccgcccgtcg gcgtcgaaga tgaacgtcgt cggtagcgac agcactgaaa
     8281 gtcggcgcgc cacagcggga ttggcgtcca tgtcgatttc aacgtgagcc actgcgggaa
     8341 ggtcaccgca cacctggttg accacccgac gcaccgccgc gcacggccca caccacactg
     8401 cgctgaagtg cacgactgtc gggcccgtcc tggacaggcc gagatcggca ccgtggtcca
     8461 ccacttgcat gctgtcccgc agcacgcccg acctgcggtt gaccaggacg ccgatcaccg
     8521 cagcgacacc gagcgccgcg gctatcacgc acccggcaag gaccagcgaa gagctcatga
     8581 cactgtgaac tcgtcgaggt gcacggttag tccctcgacg atcccttcga tgatgacatc
     8641 tgagccccgc gcaccctctc ggttcggggc gataccgaac ggcagcttct gcccgggcag
     8701 gttcttgctg aacgccttca gtaccgcgtc gagcttctcg tcgggcactg ttcggtcggc
     8761 ggtaccgggc ccggtgagga tgccggtggc ggtgaacgcc aacgtggtgt cgccgggtcc
     8821 ggtcatggac aggtccaccg agacactcac ccgctcctgc aggccggcgg ccttcggcgt
     8881 tccggtgaac accagcccga cgctgctgga gatcccagac tcggtggtgc cgccggtcgc
     8941 gtcgttggtc tccttgggcg gggcctcgac caccaggtcg ttgataccca tgaaccggcc
     9001 gaggtgagtg gagtcgatga tgatccggct ttcgagcttg ccgacgggaa gcggcgcgcc
     9061 gggccggatc agccacgacg cctgcgtcag gtcgacctcg tgcatcgtcg cctccagcga
     9121 cgcgctgccg acgatcggcc ggtcgacgcc gctggccttg atctcgactt cgtcgtaacg
     9181 gtgccgaagc gcctgcggaa tgaacgggaa cccgaggatg ccgacccacg ggtcgaaccc
     9241 gagacccgcc tcggcgcgaa gggtgctcgc cagccggtac tcggcgtaga tcgcagtgcc
     9301 gaaatcggct ccgacggcac cgccggcgag cgcgacgaac atcacagtga tcccgatcag
     9361 cagcgcacgc acccgcacat tctggcgtac tgggatgcag tgtccgccct gcagacggct
     9421 aagtcgcagg tcgcgggtta tcgttagatg accgcgcgga cgcgaggagc aggtaggacg
     9481 tcagttcgac gaccggactc acatccgtag acccgcgcgc tggagggtgt gttggattta
     9541 ttgctactca ccggggaccc gcatccggag gcggtgctgc cctcgttgtc gctgctgccg
     9601 cacaccgtgc ggagagcgcc gagcgaggtc tcttctctgc tggacgcggg cagcgccgac
     9661 gttgcgatcg tcgatgcgcg cacggatctc gccgccgcgc gggggctgtg ccggctgctc
     9721 gggaccaccg ggacattggt tccggtggtg gccgtcgtca acgaaggtgg cctcgtcgcg
     9781 atcaacgtcg actggggact cgacgacatc ctgctgcccg gcaccggacc ggccgagatc
     9841 gacgcgcggc tgaggctgct cgtcggccgc cgcggcgggg cggccaacca ggagaacgtc
     9901 ggcaagatca cgctcggcga gctcgtgatc gacgaaggca cctacaccgc gcggctgcgg
     9961 ggccggccgc tcgacctgac gtacaaggaa ttcgagctgc tcaagtactt ggcgcagcac
    10021 gccggccggg tgttcacccg cgctcagctg ctgcaggagg tctggggcta cgacttcttc
    10081 ggcggcaccc gcactgtcga cgtgcacgtc cgtcggctgc gggccaagct cggcaccgaa
    10141 tacgagtcgc tgatcggcac ggtgcgcaac gtcggataca aggcggtgcg ccccgcccgg
    10201 ggccggccgg cggccgatga agagctcgac gacgacagcg aggccgagtt cgcgcctgcg
    10261 gcaaacgaac tcccgcagtc ggtatccgac gctcttcgcg caagcggccc ggcacctgat
    10321 ccgctgcgca ctccgtgaca gacatcgact ggcggtcgag tctcgcgcca ggcgacgaac
    10381 accaagttcg tgagctgatc gctgcggccg cccgcgccga cggcgtcgcg ccggtgggcg
    10441 agcaggtggt gcgtgaactt ccgttgcagc ggaccaagca cctggtggcc gccgacgggc
    10501 agcgcgtcgt cggctacctc aacctcgtac cggaaaccga gacggcgccg ccgatcgccg
    10561 agctggtggt ggcgcccgat gtccggcgtc gtggcgtcgg tgcgtcgatg gtgcgggccg
    10621 cgatcgacgc gggcggcgac gccacccgga tctgggcgca
//
LOCUS       DASWBF010000236         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_150428,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000236 DASWBF010000000
VERSION     DASWBF010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150428"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1743)
                     /locus_tag="VF874_08600"
     CDS             complement(22..1743)
                     /locus_tag="VF874_08600"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX9176140.1"
                     /translation="MDGTTQEAPVTEKPFALSSQTLGALPIVDRFLARMRVDALLDRF
                     MPARDARVALAPAKAIGLLVRNLCVSREPVYALGEWAAPLDPPLLGMTPDEVQLLNDD
                     RVGRALDRLFDADRASLLTQLTLGVIAEFNVDCSQLHNDSTSITLTGAYRAADGHQRA
                     GKPTAAAARGHNKDHRPDLKQLLWILTVSADGSVPVAHRLTDGNTNDDTTHIATWDEL
                     RAIVGRADFLYVADCKLCTREQMTHIDKRGGRFVTVLPRSRREDGFLRDWAHTSTLEW
                     TEALRRPGKRRDDPDETWHVAPAPIPSSEGHRIVWVRSSQKTRIDADIRQQRIERGLD
                     ALGALNERLAGPKSRLRDRASVEQAATAALTGAIAERWITTAVIETTEETIRQENRGR
                     PGKNTRYRKITRIHWHVQHAIDATTVAYDAASDGCFPLISNDHHLTEAELLIAYRYQP
                     NLEKRHHQLKTVQHAAPVLMKNPERIEALFLCQYVALLCCCLIERDLRNAMRRQQIND
                     LPLYPEQRACTQPTAARTLELFDGLARHHLARHDGRHVQTFPPKLTNLQQQLLALLDI
                     PEDTFTT"
     gene            1787..2128
                     /locus_tag="VF874_08605"
     CDS             1787..2128
                     /locus_tag="VF874_08605"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HEX9176141.1"
                     /translation="MPSQPAYHDHQQRDLIDQIAALGPCLPGSITTRQMRCGRARCAC
                     KADPPKLHGPYTYWTRKEQGRTIARLLNTEQIERYQPQIDNARRLRQLVTTLETLAVT
                     TAENAEQWGRK"
     gene            2113..2508
                     /locus_tag="VF874_08610"
     CDS             2113..2508
                     /locus_tag="VF874_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176142.1"
                     /translation="MGAQIALKKCGTSDTRHPSEDFEAVGQYIGAQVAAVVSALGTSD
                     DPVGYGQSVARRLFPDLLPYVLGTPASYGFAAFNGRTLADNAPEAMLSLVLGTAVPSG
                     LKPAVAEQLRDASFPYVVPVHRLVGARDW"
BASE COUNT          373 a          835 c          929 g          441 t
ORIGIN      
        1 gcgtggtgtt gggtggtgtt gtcaggtggt gaaggtgtcc tcgggaatgt ccaggagggc
       61 gaggagttgt tgctggaggt tggtgagttt gggtgggaag gtctggacgt gtcggccgtc
      121 gtggcgggct aggtggtgac gtgcgagccc gtcgaagagt tcgagcgtgc gggcggcggt
      181 tggctgggtg caggcgcgct gttcggggta gagcggcagg tcgttgatct gctggcggcg
      241 catcgcgttg cgtaggtcgc gttcgatcag gcagcagcac agcagcgcga cgtactggca
      301 gaggaagagc gcttcgatcc gctcggggtt cttcatgagc acgggggcgg cgtgctggac
      361 ggtcttgagc tggtggtggc gcttttcgag gttgggctgg tagcggtagg cgatcagcag
      421 ctcggcttcg gtgaggtggt ggtcgttgct gatcagcggg aagcagccgt cgctggcggc
      481 gtcgtaggcg accgtggtcg cgtcgatggc gtgctggacg tgccagtgga tgcgggtgat
      541 cttgcggtag cgggtgttct tgccgggtcg gccgcggttc tcttggcgga tggtctcctc
      601 ggtcgtctcg atgacggccg tggtgatcca gcgctcagcg atcgcgcccg tgagcgcggc
      661 ggtcgcggcc tgctcgacgc tggcgcggtc gcgtagccgg ctcttggggc cggcgaggcg
      721 ctcgttgagc gcgcccagcg cgtcgaggcc gcgctcgatg cgctgctggc gaatgtcggc
      781 gtcgatgcgg gtcttctggc tggagcgcac ccagacgatc cggtgcccct cgctggaggg
      841 gatcggcgct ggcgcgacgt gccacgtttc gtcgggatcg tcgcggcgct tgcccggccg
      901 tctgagcgcc tcggtccact caagcgtgct ggtgtgcgcc cagtcgcgca ggaagccgtc
      961 ctcgcggcgg ctgcggggca gcacggtcac gaaccgtccg ccgcgcttgt cgatgtgcgt
     1021 catctgctcg cgcgtgcaca gcttgcagtc ggcgacgtag aggaagtcgg cgcgcccgac
     1081 aatcgcccgc agctcgtccc aggtggcgat gtgcgtggtg tcgtcattgg tgttgccgtc
     1141 ggtcaggcgg tgcgcgactg ggaccgaccc gtcggctgag acggtcagga tccacagcag
     1201 ctgcttgaga tcgggacggt ggtccttgtt gtgaccacgc gccgcggccg ccgtcggctt
     1261 gccggcgcgc tgatgaccgt cggcggcgcg gtaggcgccg gtcaacgtga tcgacgtcga
     1321 gtcgttgtgc agttgcgagc agtcgacgtt gaactcggcg atcacgccga gcgtcagctg
     1381 cgtgagcagc gacgcgcggt cggcgtcaaa cagtcggtcc agcgcgcggc cgacgcggtc
     1441 gtcgttgagc aactgcacct cgtccggcgt catgcccaac agcggcgggt ccagcggggc
     1501 cgcccactcc ccgagcgcgt acaccggctc gcgcgacacg cacagattgc gcaccagcag
     1561 gccgatcgcc ttggccggcg cgagcgcgac gcgggcgtcg cgggccggca tgaagcgatc
     1621 cagcaacgcg tcgacgcgca tccgcgcgag gaagcgatcg acgatcggca gcgcgcccag
     1681 cgtctggctg gacagcgcga acggcttctc ggtcaccggc gcctcctgtg tcgttccatc
     1741 catctcctac tgtagcttga tagtaggatc ggacggaacg accgagatgc cctcccagcc
     1801 cgcataccac gaccaccaac agcgcgatct aatcgaccag atcgccgcgc tcggaccgtg
     1861 cctgcccggc agcatcacca cccgccagat gcgctgcggc cgcgctcgct gcgcctgcaa
     1921 ggccgacccg cccaagctcc acgggcccta cacctactgg acccgcaaag aacagggccg
     1981 cacgatcgcg cgcctgctca acaccgagca gatcgagcgc taccagcccc agatcgacaa
     2041 cgcacgccgc ctacgtcagc tcgtcaccac cctcgaaaca ctcgcggtca ccacggccga
     2101 gaacgccgaa cagtgggggc gcaaatagcg ctcaagaagt gcggaacgtc agatacacgg
     2161 catccgtccg aggatttcga agccgtcggc cagtacattg gcgcccaagt cgccgccgtc
     2221 gtgtcggccc tcgggacctc agacgacccg gtgggctacg gccaatctgt ggctcgaagg
     2281 ctgttccccg acctcctgcc atacgtactc ggaacgccgg cgtcctacgg cttcgccgcc
     2341 ttcaacggcc ggacgctggc cgacaacgcc cccgaagcga tgctgtcgct cgtcctgggc
     2401 acggccgtgc cgtcggggct gaagccggct gtagctgagc aactgcggga cgcctccttc
     2461 ccgtacgtcg ttccggtcca ccggcttgtc ggcgctcgcg attggtaggc atccgcaatc
     2521 actggggcgc ggcacacgga aggcaaatcc ccgccgcgcc ccgcggccgc catttctc
//
LOCUS       DASWBF010000237         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1135659,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000237 DASWBF010000000
VERSION     DASWBF010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135659"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VF874_08615"
     CDS             <1..302
                     /locus_tag="VF874_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX9176143.1"
                     /translation="IDVADIDALADAYSAGGADVTYHRDAFCEHLLLHPLSAPMTLRW
                     LSDRFAGRPLTDHLIRTKWPTMFNPATYLGIARLAVFAAEVLTGRTVRRRKLTVP"
     gene            421..1965
                     /locus_tag="VF874_08620"
     CDS             421..1965
                     /locus_tag="VF874_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX9176144.1"
                     /translation="MSEQRRDVGAVQGTVRGTVETSAGDAREAIAALREHEAWAIIRR
                     STRAGDRDTVGLVGGRRQVVESVLDVPLEDGPPNAGHVADRLLAIPFRQVAERGFEAH
                     DDGTPLVVVDVESEREFTVAAVVAAIADTGVALADRGGFETGDDEYAKLVEAVITDEI
                     GQGEGANLVIGRHYRATVADWGPDNALTVFRRLLQRERGAYWTFLFFTGDRYLIGASP
                     ERHVTVHGGDVRMNPISGTFRIPAVDGDGAGDLKSQLLDFLADEKEIYELFMVVDECA
                     LCTEGGQVLGPFLKPMSRLVHTEYLLAGRTTRDPRDVLRDTMYAATVTGSPVQNACRL
                     IKRYESRGRGYYGAALAVLGRDASGGPVVDSPIVIRTADVDLDGHLTVTAGATLVRDS
                     DAADEVAETHAKAGGILSAFGLVPPAPVPSVNVAELVNDEDVLLVLNARNRRLSTFWL
                     TDQAGTPPDPQLRGKHVVVFDGEDDFVNMLRHVLGVLGLTSSVIRHDGIVNLTGAQPN
                     RGVRIG"
     gene            complement(1988..2533)
                     /locus_tag="VF874_08625"
                     /pseudo
     CDS             complement(1988..2533)
                     /locus_tag="VF874_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296700.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          383 a          882 c          882 g          386 t
ORIGIN      
        1 tcatcgatgt cgccgacatc gacgcgctgg ccgacgccta ttcggccggc ggagccgacg
       61 tcacctacca ccgggacgcg ttctgcgagc atctgctgct gcacccgctg tccgccccga
      121 tgacgctgcg gtggctgtcg gaccggttcg ccggccgacc cctgaccgat cacctgatca
      181 gaacgaagtg gccgaccatg ttcaacccgg caacctacct gggcatcgcc cggctggccg
      241 tgttcgcggc ggaggtcctc accggcagga cggtccgccg ccgtaagttg acggtgccct
      301 gagaagtcag cgacgggtcg gcgacgcacg acgtgaagca gcggcgtcgt atgacgtcaa
      361 gcagcaacgg tcggttggga cgggcaagca acaacgggtc ggcggacgta gggtcggcgc
      421 atgtccgagc agaggcgcga cgtcggggcc gtgcagggga ccgtgcgggg aaccgtggag
      481 acgtctgcgg gcgacgcccg cgaggcgatc gcggcgctgc gggagcacga ggcctgggcg
      541 atcatccggc gctcgacccg ggcgggcgac cgcgacaccg tcggcctcgt cggcggtcgg
      601 cgccaggtgg tcgagtccgt actcgacgtg ccgctcgagg acgggcctcc gaacgccggt
      661 catgtcgccg accggctgct ggcgatcccg ttccgccagg tcgccgagcg cggcttcgag
      721 gcccacgacg acggcacgcc gctggtcgtg gtcgacgtcg agtccgagcg cgagttcacc
      781 gtcgcggccg tggtggcggc catcgccgac accggtgtcg cgttggccga ccggggcggc
      841 ttcgagaccg gcgacgacga gtacgccaag ctcgtcgagg cggtcatcac cgacgagatc
      901 ggccagggtg agggcgccaa cctggtcatc ggccggcact accgcgccac cgtcgccgac
      961 tggggtcccg acaacgccct cacggtcttc cgccggctgc tgcagcgcga gcgcggcgcc
     1021 tactggacct tcctgttctt caccggcgac cgctacctca tcggcgccag tcccgagcgg
     1081 cacgtcaccg tgcacggcgg cgacgtgcgg atgaacccga tcagcggcac cttccgcatc
     1141 cccgccgtcg acggcgacgg cgcgggtgac ctgaagagcc agctgctgga cttcctggcc
     1201 gacgagaagg agatctacga gctgttcatg gtcgtcgacg agtgcgccct gtgcaccgag
     1261 ggcggccagg tgctcgggcc gttcctcaag ccgatgagcc gcctggtgca caccgagtat
     1321 ctgctcgccg gacgcaccac ccgcgacccg cgcgatgtcc tgcgcgacac catgtacgcc
     1381 gccacggtca ccggctcgcc ggtccagaac gcctgccggc tgatcaagcg ctacgagtcc
     1441 cgtggtcgcg gctactacgg cgccgccctg gccgtgctcg gccgcgacgc ctccggcggg
     1501 ccggtcgtcg acagcccgat cgttatccgc accgccgacg tcgacctcga cggccacctg
     1561 accgtcacgg ccggggccac cctggtgcgc gactccgacg cggccgacga ggtggccgag
     1621 acccacgcca aggccggcgg catcctcagc gccttcggcc tggtgccgcc cgcacccgtc
     1681 cccagcgtca acgtcgccga gctcgtcaac gacgaggacg tgctactggt tctcaacgcc
     1741 cgcaaccgcc ggttgtcgac gttctggctc accgaccagg ccggcacgcc gcccgacccg
     1801 cagctgcgcg gcaagcacgt cgtggtgttc gacggcgagg acgacttcgt caacatgctg
     1861 cgccacgttt tgggcgtact gggcctgacc agcagcgtca tccgtcacga cggcatcgtc
     1921 aacttaactg gggcccagcc gaatcgcggc gtgaggatcg ggtgaggggg atccggagtc
     1981 gcaccgttca ggctgcggcg gacagaccgg tgatctcgtt ccatacagcg aatcggtcgg
     2041 tcatctcgcg tcgccattcg gatgcagtga gcatgtgtcg ccgcagccgg aagtgcggtg
     2101 tgatgccgct gaacgccgag aggaaccgct gcgcctgccg gggcgaagcg aaccgtttca
     2161 tcgcccgctc gcgttgtcgc gtgggctgat gggagttctc ggcgcgaatg agttcagata
     2221 gcgggaccgg cgatgctcgg ccgaggacag cagttcgcgg tgcgcgacct ggtagctgcc
     2281 cagcttgtcg gtgaccagca cgcgcggcac atactgcagt cccttgagga gccgccggaa
     2341 gaactttttg gcggccgcag cgtttcggcg ggactggacc agcacgtcga ggacgttgcc
     2401 gtcttggtcg acggcgcgcc agaggtagtg gtgctttccg ttgatccgga tgaacacctc
     2461 gtccagatgc cacttgtcac ccggtcgggg ccgccgccgg cgtagctggt tggcgtagcc
     2521 ctggccgaac ttg
//
LOCUS       DASWBF010000238         6167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_486284,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000238 DASWBF010000000
VERSION     DASWBF010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486284"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VF874_08630"
     CDS             complement(<1..412)
                     /locus_tag="VF874_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9176145.1"
                     /translation="MLTTKRLTGWGRTAPTVAQVLCSPDPEEIAKAVAKAGTRGVIAR
                     GLGRSYGDNAQNGGGLVIDMTPLNRIHSINSDDGLVDVDAGVNLDQLMRAALPFGLWV
                     PVLPGTRQVTVGGAIACDIHGKNHHSAGSFGNHVR"
     gene            complement(556..729)
                     /locus_tag="VF874_08635"
     CDS             complement(556..729)
                     /locus_tag="VF874_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176146.1"
                     /translation="MLGAYVRQLGLQLRHGGGCLLGQLLHRTDHRGIVENLGGGRVDL
                     AAGGVGRRGVPAG"
     gene            699..887
                     /locus_tag="VF874_08640"
                     /pseudo
     CDS             699..887
                     /locus_tag="VF874_08640"
                     /inference="COORDINATES: protein motif:HMM:NF018018.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            938..1090
                     /locus_tag="VF874_08645"
     CDS             938..1090
                     /locus_tag="VF874_08645"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HEX9176147.1"
                     /translation="MRISFGILEGQADRLRADLEDLADKLRAAADAYENQELESSAAL
                     DIVSTL"
     gene            complement(1087..1497)
                     /locus_tag="VF874_08650"
     CDS             complement(1087..1497)
                     /locus_tag="VF874_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX9176148.1"
                     /translation="MAEPLLPPTLSLKTQILRFLITGGFAAVVDFGLYVAGLSVGLNV
                     NVAKSLSFVAGTATAYLINRRWTFQAPPSRARFVAVCVLYGLTYAVQVGINYVFYMQF
                     EDQPWRVPVAFVIAQGTATVINFVVQRAVIFRLR"
     gene            1572..2780
                     /locus_tag="VF874_08655"
     CDS             1572..2780
                     /locus_tag="VF874_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9176149.1"
                     /translation="MHTSTAIALTLLVLAYAVVSGLVRRWYVAPALIFVVFGVALGPS
                     ALGVIDVQNADAQSFTILAQLALTVILFNQASRLNLRTELRRGHLTVRLLAIGMPVTV
                     VLGALTAAVLLPVLPVSEAVCLAVIVAPTEAALIEAVLEDRRVPEQVRHALSAESGFY
                     DGFALAALLAALAVASEQKDPELAHWVWFGVRTELLSVAVGIAIGSIGTAVIVRSRAR
                     GWMSPTWAQLATLALALLSFATGERLHASGFVAAFAAGLAFAQVLSMSAETVESTRVS
                     DAVGELLELLVFAMFGAFAVISAWRDVGWRVVVFAVVAVFAVRVVAVLLAMIRSSVPL
                     MNTLFMGWFGPRGIGTLVLGLLVVDRDHIQQGNVIAQAVVVTVTLSLVLHSISAPLAI
                     RMAAKSAEPV"
     gene            2879..3124
                     /locus_tag="VF874_08660"
     CDS             2879..3124
                     /locus_tag="VF874_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176150.1"
                     /translation="MTIVTPAVSFADCDNTQWWDPVASVCRPLVVAPLDCENGSWWDP
                     VGNVCRPLVTQPLGCDFGSYWDPVANVCRTVFVLPPE"
     gene            3168..3611
                     /locus_tag="VF874_08665"
     CDS             3168..3611
                     /locus_tag="VF874_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9176151.1"
                     /translation="MHAGDIVEDFSVVTMDSPALDAARMLAEHLLPGVVVTDAEGRPY
                     AVLPASQVVRFIVPGYVQDDPSLAGVLNESMADKCAERLGGKKVRDVLPEHLLDVPSV
                     DAGDTIIEVAAIMARHRSPLLAVMNRGELRGVITASRLLAAALGR"
     gene            3707..4996
                     /locus_tag="VF874_08670"
     CDS             3707..4996
                     /locus_tag="VF874_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX9176152.1"
                     /translation="MTVVAITVFVVAYAFIASDRVNKTLVALSGAAVVTALGVVESDD
                     VFFSRETGIDWDVIFLLLGMMVIVSVLRQTGVFEYIAIWAAKRAKGSPLRIMILMVLV
                     TAFASALLDNVTTVLLVAPVTLLVCDRLNIRAAPFLMAEVFASNIGGASTLVGDPPNI
                     IIASRAGLSFNDFLIHMTPIVVVVMAVFVAMLPRLFRGSFDVDPDRVADVMSLEEREA
                     IRDPALLIKCAVVLVAVFAAFIAHSALHIAPSIVALLGAGVLVLISRTEKSVYLSGVE
                     WETLLFFAGLFIMVGALVKTGVIDQLARLATDATGGNALLTVMLILGVSAPVSGVIDN
                     IPYVATMTPIVAELATSMHGEVHQNALWWALALGADFGGNLTVVGASANVVMLGIARR
                     SDSPISFWEFTRKGVVVTVMSISLAALYLWLRYFVLN"
     gene            complement(5082..5915)
                     /locus_tag="VF874_08675"
     CDS             complement(5082..5915)
                     /locus_tag="VF874_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9176153.1"
                     /translation="MTTITEDAVDSKTFHRAFQDLSDGLKRRQLWLHLGWQDIKQRYR
                     RSVLGPIWITIATGTTAVAMGGLYSKLFHLELSEHLPYVTLGLIIWALISASILEGAE
                     VFVANEGLIKQLPTPLSVHVYRLVWRQAILFAHNIVIYVIIAMIFPKPWKWTDLSVIP
                     ALLLIMLNCIWASLVFGIIGTRFRDIGPLLNSIVQLLFFMTPIIWNDQTLQAQGAGQW
                     RWIVELNPLFHYLDIVRAPLLGADQHARHWIVVLALTAIGWVLAVLAMRQYRSRVPYW
                     V"
     gene            complement(5912..>6167)
                     /locus_tag="VF874_08680"
     CDS             complement(5912..>6167)
                     /locus_tag="VF874_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactofuranosyl transferase"
                     /protein_id="HEX9176154.1"
                     /translation="EFKPILGGRMHTQYPDDPNKRFFTYRNRGYVLSQRGLRRLLPQE
                     WLRFGWFFLVSRRDPGGLLEWIRLRRLGRAEKFGRPEEGA"
BASE COUNT          969 a         1999 c         2068 g         1131 t
ORIGIN      
        1 accggacgtg gttgccgaag ctgcccgcgc tgtggtggtt cttgccgtgg atgtcgcacg
       61 cgatcgcgcc gcccacggtg acctgacggg tgcccggcag caccggcacc cacagcccga
      121 acggcagtgc ggcgcgcatc agctggtcca ggttcacgcc ggcatccaca tcaacgaggc
      181 cgtcgtccga attgatcgag tgaatccggt tcagcggcgt catgtcgatc accaggccgc
      241 cgccgttctg cgcgttgtcg ccatacgacc ggcccagccc gcgggcgatc accccgcggg
      301 ttccggcctt cgcgaccgcc ttcgcgatct cctcggggtc ggggctgcac agcacctgcg
      361 cgacggtggg tgcggtgcgg ccccaccccg tcagccgctt cgtggtcaac atcgccggtc
      421 agcctacctc cgcacacaag cgtcagaccg gcggaattcc gcgcggggct gttcccggac
      481 ctgcggtagc gtttccggca tggaggagtc gccggtttgc tgctgagcga cgccgacttg
      541 gacgtgatcg ctgggctacc cggctggaac tcccctgcgg ccgacgccgc ccgcggcaag
      601 atccactcga ccaccgccaa ggttctcgac gatgccgcgg tgatcggtgc ggtgcagcag
      661 ctggcccagg agacagccgc cgccgtggcg aagctgcaat ccgagttggc ggacatacgc
      721 acccagcaag ctcgagcacg gcgacatcac cgcacggggc gcgacggatg tggccgccgc
      781 cgagcgtgca aaacgaagcc gagaaccagc tcttattggc gggtcgccac gacgagaccg
      841 tggcgacgat tgcctggctc ggctacgacc cgccgggccg tcgccgacga aatgtcgggg
      901 cacacctctg cgtggcaggc cgcgggcaga gccggctttg cgaatttcat tcggcatcct
      961 cgaggggcag gccgaccggc tccgcgctga tctcgaggac ctcgccgaca agctgcgggc
     1021 cgccgccgac gcctacgaga accaagagct ggagtcgagc gccgctttgg acatcgtgtc
     1081 cacgctctag cgcagccgga agatcaccgc gcgctgcacg acgaagttga tcaccgtcgc
     1141 ggtgccctgc gcgatcacga acgcgaccgg cacccgccac ggttggtcct cgaattgcat
     1201 gtagaagacg tagttgatgc ccacttgcac ggcgtaggtc agcccgtaga gcacgcagac
     1261 cgcaacgaac cgcgccctgc tgggcggcgc ctggaatgtc catcggcggt tgatcaggta
     1321 cgcggtggcc gtacccgcga cgaagctcag cgacttggcc acgttgacgt tcagcccaac
     1381 ggacagcccg gccacataca gcccgaagtc gacgaccgcc gcaaagcccc cggtgatgag
     1441 gaaacgcaga atctgcgtct tcagggacag cgtcgggggg agtaacggct cggccacccg
     1501 ggcagcttaa agggtatcga tgagccgctt gcgcgaagag ctatggcagg gctgtgcgaa
     1561 cctgaacgtc gatgcacacg tccactgcga tcgcgctgac cttgttggtg ctggcgtacg
     1621 cggtggtctc cggcctggtg cggcggtggt acgtggcgcc cgcgttgatc ttcgtcgtct
     1681 tcggcgtggc gctgggcccg tcggcgctcg gtgtgatcga cgtgcagaac gccgacgccc
     1741 aaagcttcac gatcctggcg cagctcgcgc tgaccgtcat cctgttcaac caggcatcgc
     1801 ggctcaacct gcgcaccgag ctgcggcgtg gccacctgac cgtgcggttg ctggcgatcg
     1861 ggatgccggt gacggtcgtg ctcggtgcgc tcaccgcggc ggtcctgctg ccggtgttgc
     1921 cggtgtcgga ggcggtgtgc ctggcggtga tcgtcgcgcc gacggaggcc gcactgatcg
     1981 aagcggtgct cgaggaccgt cgcgtgcccg aacaggtccg ccacgcgctg tcggcggaga
     2041 gcggcttcta cgacggcttc gcgctcgcgg cgctgctcgc cgcgcttgcc gtcgcatccg
     2101 aacagaagga tcccgagctg gctcactggg tctggttcgg ggtccgcacc gaactccttt
     2161 cggtcgctgt cggcatcgcg atcgggtcga tcggaacagc ggtcatcgtc cgctcccgcg
     2221 cacgcggctg gatgagcccg acttgggcgc agctggcgac gctggcactg gcgctgctct
     2281 cgttcgcgac gggagaacgg ctgcacgcca gcggcttcgt cgcggcattc gcagcggggc
     2341 tggcgttcgc gcaggtcttg agcatgtcgg ctgaaacggt ggagtcaacc cgggtgtccg
     2401 acgccgtcgg cgagctgctc gaactgctgg tgttcgcgat gttcggggcc ttcgccgtga
     2461 tctcggcatg gcgcgacgtc ggctggcggg tggtggtgtt cgcagtcgtc gccgtgttcg
     2521 cggtccgcgt cgtcgcggtg ctgctggcga tgatccggtc cagcgtgccg ctgatgaaca
     2581 cgctgttcat gggctggttc ggcccgcgcg gcatcgggac gctggtgctg ggcctgctgg
     2641 tcgtcgacag ggaccacatc caacaaggca acgtgatagc gcaggccgtc gtcgtcacgg
     2701 tgacactgag cctggtcctg cacagcatca gcgcgccgct ggccattcgg atggccgcca
     2761 agtccgccga gccggtttag gtttaggaca tcggcagccg cgttacgctt ccatcgtgag
     2821 aaagtttgct cgtcgtgtgt tggtcggcgc taccgcggcg ttcgcgtcga ccgccttcgt
     2881 gacaatcgtg acaccggccg tgagtttcgc ggactgtgac aacacccagt ggtgggatcc
     2941 cgtcgccagc gtgtgccggc cgctggtcgt cgcgcctctg gactgtgaga acggctcctg
     3001 gtgggacccg gtcggtaacg tgtgccggcc attggtcacg cagcctctgg gttgcgactt
     3061 cggttcgtat tgggacccgg tggccaacgt ctgtcggacg gttttcgtcc tgccgccgga
     3121 ataggacggt cccgggctca gtggcgacgg ggtcgtatcg tgcagttgtg cacgcaggcg
     3181 acatcgtcga agacttctcc gtggtgacca tggactcgcc cgctttggac gccgcccgga
     3241 tgctcgccga gcatctgttg cctggcgtcg tcgtgaccga cgccgaaggc cgtccatacg
     3301 cggtgctgcc ggcctcgcaa gtcgtgcgat tcatcgtgcc gggctacgtg caggacgatc
     3361 cgtcgctggc cggggtgctc aacgagtcga tggccgacaa gtgcgcagaa cggctgggcg
     3421 gcaagaaggt acgcgacgtg ctacccgaac acctgctcga cgttccgtcc gtcgatgcgg
     3481 gcgacacgat catcgaagtc gccgcgatca tggcgcggca ccgaagccca ttgctcgccg
     3541 tgatgaaccg cggcgaactg cgcggtgtga tcaccgcatc gcgtctgctg gctgccgcac
     3601 tcggtcggta gcttcgcgca attgataggt ttgtcctcgg tgtgccggga agcctggtcg
     3661 gcgtcagttt cgaaaaagac ttagccctac ggccaggagt agcccgatga ccgtcgtcgc
     3721 gatcaccgtg ttcgtggtcg cctacgcgtt catcgccagc gatcgtgtca acaagacgtt
     3781 ggtcgcgttg tccggcgcgg cggtcgtcac cgcactcggt gtcgtggaat ccgacgacgt
     3841 gttcttctcc cgcgagaccg ggatcgactg ggacgtcatc tttctgctgc tgggcatgat
     3901 ggtgatcgtc tcggtgctgc ggcagaccgg cgtcttcgag tacatcgcga tctgggcagc
     3961 caagcgcgcc aagggatctc cgctgcgaat catgatcctg atggtgttgg tgacggcgtt
     4021 tgcgtcggcg ttgttggaca acgtcaccac cgtgttgctc gtcgcgccgg tgacattgct
     4081 ggtgtgcgat cgactcaaca tccgcgcggc accgtttctg atggccgagg tgttcgcgtc
     4141 caacatcggc ggcgcgtcga cactggtcgg tgaccctccg aacatcatca tcgcaagccg
     4201 agcgggattg tcgttcaacg actttctcat ccacatgaca ccgatcgtgg tggtcgtgat
     4261 ggcggtgttc gtggcgatgc tgccgcggct gttccgcggt tcgttcgacg tcgacccgga
     4321 tcgcgtggcc gacgtgatgt cactcgaaga gcgcgaagcg atccgcgatc ccgcactgct
     4381 gatcaagtgc gccgtggtgc tggtcgcggt gttcgcagcg tttatcgctc actcggcgtt
     4441 gcacatcgcg ccgtccatcg tcgcactgct cggcgccggc gtgctcgtgt tgatcagccg
     4501 gacggagaag tccgtctacc tgtccggcgt cgaatgggaa acgctgctgt tcttcgcggg
     4561 cctgttcatc atggtgggcg cgctggtgaa gaccggggtc atcgatcagc tcgcgcgact
     4621 ggcgacggac gccaccggcg gcaacgcgtt gctcacggtg atgctgatcc tcggtgtttc
     4681 ggcgccggtg tccggcgtca tcgacaacat cccctacgtc gcgacgatga cgccgatcgt
     4741 cgccgagctg gccacgtcca tgcacggcga ggtgcatcag aacgcgctgt ggtgggcgct
     4801 ggccctgggc gcggacttcg gcggcaacct gaccgtggtc ggcgccagtg cgaacgtcgt
     4861 gatgctgggc atcgcccgcc ggtccgacag cccaatctcc ttctgggagt tcacccgcaa
     4921 gggtgtggtt gtcacggtga tgtctatttc gcttgcggca ctgtaccttt ggctgcgcta
     4981 cttcgtcctc aactagctcc gcgaaatagc attccgggct gtgatcagcc gggaatcacg
     5041 accctgtgtt ctgtctcgcg acgctgtgaa gatcccggga actacaccca gtacggcacc
     5101 cggctgcggt actgccgcat cgccagcacc gccagcaccc acccgatcgc ggtcagcgcc
     5161 agcaccacga tccagtgccg cgcatgctgg tccgcgccga gcaacggtgc ccgcacgatg
     5221 tcgaggtagt ggaacagcgg gttcagttcc acgatccagc gccactgccc cgcgccctgc
     5281 gcctgcaagg tctggtcgtt ccagatgatc ggcgtcatga agaacagcag ctgcacgatg
     5341 ctgttcagca gcggaccgat gtcccggaac cgggtgccga tgatgccgaa gaccagtgac
     5401 gcccagatgc agttcagcat gatcagcagc agcgccggga tcaccgacag gtcggtccac
     5461 ttccacggtt tcgggaagat catcgcgatg atcacgtaga tcacgatgtt gtgcgcgaac
     5521 aggatcgcct gccgccacac caggcggtag acgtgcaccg acagcggggt cggcagctgc
     5581 ttgatcagtc cctcgttggc gacgaacacc tcggcgccct ccaggatcga cgcgctgatc
     5641 agcgcccaga tgatcaagcc caacgtgacg tagggcagat gctcggagag ctcgaggtgg
     5701 aacagcttgg agtacaggcc gcccatcgcg acggcggtgg ttccggtggc gatcgtgatc
     5761 cagatcggcc cgagcaccga gcgtcggtag cgctgcttga tgtcctgcca gcccaggtgg
     5821 agccacagct ggcgacgttt cagcccgtct gacagatcct ggaaggcccg gtggaacgtc
     5881 ttggagtcca cggcatcctc ggtgatagtc gtcacgcgcc ctcctctgga cgtccgaatt
     5941 tctccgcccg cccgagccgc cgcagacgaa tccattcgag aagtccgccc gggtcgcggc
     6001 gcgacaccag gaagaaccag ccgaaccgca gccactcctg cggcagcagc cggcgcagcc
     6061 cccgctgcga cagcacgtat ccgcggttgc ggtacgtgaa gaaccgcttg ttcggatcgt
     6121 cgggatactg cgtgtgcatg cgaccgccga ggatcggctt gaactcg
//
LOCUS       DASWBF010000239        17989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_222876,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000239 DASWBF010000000
VERSION     DASWBF010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17989
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222876"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VF874_08685"
     CDS             <1..347
                     /locus_tag="VF874_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176155.1"
                     /translation="MTTSSGGAPVVTPEPPTNSGPPSITDNGPGPIRTMLNDAFSMVP
                     GKPQTASGSNSSATSPKQPKAWKPGDVLRRVTAPLREAIDGLTKPRTTTPPGGDVQGP
                     PPSDNGEGQAAA"
     gene            complement(344..1246)
                     /locus_tag="VF874_08690"
     CDS             complement(344..1246)
                     /locus_tag="VF874_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HEX9176156.1"
                     /translation="MADKRFTKVGDDMQPHFEDIQAHYDLSDDFFGVFQDPTRKYSCA
                     YFTGPDVTLSEAQIADVDLNLEKLDLKPGMTLLEVGCGWGLTLQRAMEKYDVNVIGLT
                     LSKNQQAYCDKLLAKADSDRKFDVRLEGWEQFHEPVDRIVSIEAFEHFGFERYDDFFK
                     TCFDILPDDGRMTIQSSVGFHPFDLADKGKKLTFELARFVKFIITEIFPGGRLPTTKM
                     MVEHGEKAGFVVPEPQSLRTHYIKTLGLWADALERNKDAAVAATDEENYHRYMKYLKG
                     AQYYFIDESIDVSLVTYLKPGAAA"
     gene            complement(1257..2135)
                     /locus_tag="VF874_08695"
     CDS             complement(1257..2135)
                     /locus_tag="VF874_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HEX9176157.1"
                     /translation="MTKSAGRAGLKPHFADVQAHYDLSDDFFRLFLDPTQTYSCAFFE
                     RDDMSLEEAQMAKIDLSLGKLRLDPGMTLLDIGCGWGATMLRAIERYDVNVVGLTLSK
                     NQQAHLQKLFDNSPSPRSKRVLLHGWEQFDEPVDRIVSIGAFEHFGQDRYDDFFKTAH
                     EVLPDDGVMLLHTITSLTMKQALDRGVPLTFELARFVKFIMTEIFPGGQLPTIEMVQE
                     RATDAGFTVTRIQSLQPHYAKTLDVWAETLESRRDEAIATQSEEVYDRYMHYLTGCAK
                     GFRAGNIDVNQFTLEK"
     gene            complement(2258..3160)
                     /locus_tag="VF874_08700"
     CDS             complement(2258..3160)
                     /locus_tag="VF874_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9176158.1"
                     /translation="MDLRTGTAPSGDIDIHYEDMGDPNHPAVLLIMGLGAQLVLWRTG
                     FCERLVDQGLRVIRYDNRDVGLSTKLQGHRHPGSLPPKMVRHFFGARNPSVYTLEHMA
                     DDAAALLNHLDVDKAHVVGASMGGMIAQIFAARHKPRTDTLAVIFSSNNQPLLPPPGP
                     KHLMAILQKPKDSSREAIVENAIRANKIIGSPGYPRSDDAIRAEAIEGYDRSYYPAGF
                     ARHFGAILGTGSLLRYNRQIEAPTVVIHGKADKLMRPVGGRAVARAIPGARLVMYDGM
                     GHELPEELWDDIVGELKTTFAAAR"
     gene            complement(3160..4536)
                     /locus_tag="VF874_08705"
     CDS             complement(3160..4536)
                     /locus_tag="VF874_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX9176159.1"
                     /translation="MSSTKHREVAKLDSVPLFTEAARIGATGWHVTRAAGRVAGNLFN
                     KGRWQQKLVTEVPHVFADLGPTYVKFGQIIASSPGAFGEPLSREFRSLLDAVPPADPD
                     EVHKLFVEELGKDPAELFAKWDDQPFASASIAQVHYATLHTGEEVVVKIQRPGIRRRV
                     AADLQILKRGAQIVELAKLGRRLSAQDVVADFADNLAEELDFRLEAQSMDAWVAHMHA
                     SPLGKNIRVPQVYWDHTSARVLTMEHVHGIRIDDAAAIRKAGFDGTDLVKALLFSVFE
                     GGLRHGLFHGDLHAGNLYVDPDGKIVFFDFGIMGRIDPRTRWLLRELVYALLVKKDHA
                     AAGKIVVLMGAVGTVKPEAQAAKDLEKFAQPLTVKSLGDMSYAEIGKQLSTLADAYDV
                     KLPRELVLIGKQFLYVERYMKLLAPKWQMMSDPELTGYFANFMVEVSREHVDERSREE
                     HETDEVDA"
     gene            complement(4789..5385)
                     /locus_tag="VF874_08710"
     CDS             complement(4789..5385)
                     /locus_tag="VF874_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9176160.1"
                     /translation="MSALASPVADERGALREFVAYHQDAYFAIAYGLTDEQARATPSA
                     SAMSIGGVIKHATGVQQTWMQRVAAAPDAPAPDSRPFEERAAAFQDEFVMRDDQTLEQ
                     ILDAFAAQNAETLRLIDAADLDAPVPVPTDAPWFPKDLKAWSVRWVIHHLINELARHS
                     GQADVIRESIDGATMYELVAGLEGWPETDWLKPWRPAG"
     gene            complement(5397..5528)
                     /locus_tag="VF874_08715"
                     /pseudo
     CDS             complement(5397..5528)
                     /locus_tag="VF874_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101340.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
     gene            complement(5587..6390)
                     /locus_tag="VF874_08720"
     CDS             complement(5587..6390)
                     /locus_tag="VF874_08720"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX9176161.1"
                     /translation="MTLNLWGSNKWEDRRLAVVDWINEIQPDLLALQEVVRTSRLCQA
                     SWIAERTEMTAAFGAARTRDDGEFGNAVLSRLPVHGSLRHRLTDASTGNEPRAVITVE
                     ADACGRRVSFSSTHLSWRFDEGWVREQQVRDVADVVGAHSYDFPPIVCGDFNARPEST
                     EVRFIKGLHALDQRSFHLFDAFEVANPGQPGYTWSNANPFAAAEQVPDQRIDYIFVGV
                     HAEDGAGRVLTAHVVCNEPRGGGWPSDHFGVAAELSLAGGGDGRAAQAV"
     gene            6470..10678
                     /locus_tag="VF874_08725"
     CDS             6470..10678
                     /locus_tag="VF874_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEX9176162.1"
                     /translation="MHVISAESTELFAGPPDAPLQLVRVTFRGRREPVRVDGDRITTA
                     GEPRYSQGVAEVPVEVVDPVPGEQRDARVVVGGTTTPFSFAVAEPGWTMFMISHFHYD
                     PVWWNTQGAYTSVWTADPPDRQMHGFGLVRAHLEMARREPEYKFVLAEVDYLKPYWDT
                     YPEDRADLRRMIADGRVEIMGGAYNEPNTNLTSAETTIRNFVHGMGFQRDVLGADPAT
                     AWQLDVFGHDPQFPGIAADAGLTSSSWARGPHHQWGPISSAGDPRRMQFSSEFEWIAP
                     SGRGLLTHYMPAHYGAGWSLDSAPTLAEAEAAAFQLFATLKTVALTRNVLLPVGTDYT
                     PPNKWVTDIHRDWNARYTWPKFICALPREFFTAVRAELSARGAQPSPQTRDMNPIYTG
                     KDVSYIDTKQANRAAENAVLDAERFAVFASLLGGATYPHAALAKAWVELAYGAHHDGI
                     TGSESDQVYLDLLTGWRDAWELGVTARDNALALLSSVVGGSVVVWNTLAHNRTDIVTA
                     RLPEPVGPGVRVFDVTDGVEVPALVEHDGHSISWLARDVPSVGWRAYRLQASRAAIGW
                     ELLSGNEIANEHYRLTVDAARGGAVSSLVDLKSERQLIADGRVGNELAVYEEYPAHPR
                     SGEGPWHLVPKGPVISSSAFEAESVRAYRGSLGQRIVVRGRVGELLRYTQTVTLWDGV
                     ARVDCQTTVDEFTGADRLVRLRWPCPVPGALPVSEVGDAVIGRGFGLMHDRGSEAAVD
                     AAQHPWTLDNPAYGWFGLSSAARIRVGDHLRAVSVAEVITPGEAGASSLARDLMVALA
                     RAGVTATCSSAANSRYGALDVDSNLPDTRIALGGPDENSFTAEVLAAADPVFTDELKR
                     QLYAAGAARVWVAATAPLADKWVPDADLRGARALPVLVIASARLGEAVAAVVDDLTDA
                     EIVVTQDAPTELESFESRTVALLNRGMPGFAVDSDGTLHTSLMRSCTGWPSGVWIDPP
                     RRTAPDGSNFQLQHWTHTFDYALVSGDGDWRRAGLPTRSAEFSHPLRAVVGGGETAGG
                     LPHFGSLFEVEPAGAVQLAALKAAGNPLASASGQHVDPSEGVAVRLVETSGASTDVAI
                     RSGLRRVASASRVDLLEQPRLQSMATDGLTLHGFEIATFLTRLNQPKLLDADHVMLAP
                     DAEAAQPLYARYWLHNRGPAPLGGLPAVAHLHPQQSPASGRDPHSADPNSQLLLRLTA
                     ASDCSDAALHGRVRLVCPDGWTANPADLPFVLPPGEHLETDIELAVPAKTPPGLYPVR
                     AELAITGSDAPSMPAAWRQGVEDVCVMSVGDTADLAVLRLIREPEPVDVTSGGSARLA
                     VTVGTDARADLAVEAHLISPWGTWEWIGPAAVGATLPAGGSVELGFDVSPPAWVEPGE
                     WWTLVRVGCAGRLVYSPAVKVTVR"
     gene            10675..11226
                     /locus_tag="VF874_08730"
     CDS             10675..11226
                     /locus_tag="VF874_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-ACP transacylase"
                     /protein_id="HEX9176163.1"
                     /translation="MTATAATVDGVPITVDEVDAREAALRAGPIAATLPRAGTSEGRQ
                     LRRWLTQLLATERVVTTEAAARGLTASVAPSEDELLPDITARLEIGSITAAALADPLG
                     RALFADVTSAVDVTGDDVDGYQARNPGRFPDSTAVVAHLRAAARRRAFRVWLDARRAA
                     LVELAAGYEHPGDPRQPDNTHRH"
     gene            11226..12128
                     /locus_tag="VF874_08735"
     CDS             11226..12128
                     /locus_tag="VF874_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9176164.1"
                     /translation="MASLTLAIDIGGTKVAAGLVNSSGGLVRKATSPTPHGDAEAVWA
                     AARQLVVEMTSGDAVRGVGISSAGPVDLPAGTVSPINIASWGDFPIVERVAAMVPGVP
                     VRLGGDGLCMALGEHRHGAGRGAQFLLGMVVSTGVGGGLVLDGAPYDGATGNAGHVGH
                     VVVDLDGERCTCGGRGCVETIAAGPRMAAWARANGWTAGPDADAKELAEAAMSGNALA
                     LSAFRRGAAAVAAMIASVAAVCDLDLIVIGGGVAKSGALLFDPVREALGTYAQLDFIR
                     GIRVVPAELGGDAGLVGAAALVDA"
     gene            12361..12894
                     /gene="rplJ"
                     /locus_tag="VF874_08740"
     CDS             12361..12894
                     /gene="rplJ"
                     /locus_tag="VF874_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX9176165.1"
                     /translation="MAKADKAAAVADIAEQFKEATAALVTEYRGLTVSNLAELRRSLG
                     GSATYAVAKNTLVKRAASEAGIDGLDDLFAGPTAIAFVTGEPVDAAKALKKFAKDHKA
                     LVIKGGYMDGHPLTVSEVERIADLESREVLLAKLAGAMKGNLTKAAGLFNAPASQVAR
                     LLAALQQQKESQPATAE"
     gene            12922..13314
                     /gene="rplL"
                     /locus_tag="VF874_08745"
     CDS             12922..13314
                     /gene="rplL"
                     /locus_tag="VF874_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX9176166.1"
                     /translation="MAKMSTDELLDAFKEMTLLELSDFVKAFEETFEVTAAAPVAVAA
                     APGAAAGAPAEAAEEQSEFDVILESAGDRKIGVIKVVREIVSGLGLKEAKDLVDSAPK
                     PLLEKVAKDAAEDAKGKLEAAGATVSVK"
     gene            13466..14545
                     /locus_tag="VF874_08750"
     CDS             13466..14545
                     /locus_tag="VF874_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9176167.1"
                     /translation="MGIGIQVEGLTKSFGSQVIWEDVTLDVPPGEVSVLLGPSGTGKS
                     VFLKSLIGLLRPERGKILIDGTNIIESSAKELYEIRKLFGVMFQDGALFGSMNLYDNT
                     AFPLREHTKKKEGEIRDIVMEKLSVVGLDGDEKKFPGEISGGMRKRAGLARSLVLDPQ
                     IILCDEPDSGLDPVRTAYLSQLLIDINAQIDATILIVTHNINIARTVPDNMGMLFRRK
                     LVMFGPREVLLTSDEPVVRQFLNGRRLGPIGMSEEKDEATMAEEQAMADAGHHDGGVE
                     EIEGVPPQIMATPGMPERKAVERRRARVRQMMSTLPPGAQEAIRDDLEGTHKYHSTQF
                     ASMDDGRHEDGDEAPTGRIPVSRER"
     gene            15053..>17989
                     /gene="rpoB"
                     /locus_tag="VF874_08755"
     CDS             15053..>17989
                     /gene="rpoB"
                     /locus_tag="VF874_08755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9176168.1"
                     /translation="MLEGCILAVSRQSKSAVATANSSVPGAPNRISFAKLREPLEVPG
                     LLEVQTESFQWLIGSDEWREKAIARGDTANPVGGLEEVLSELSPIEDFSGQMSLSFSD
                     PRFDEVKAPVNECKEKDQTYAAPLFVTAEFINNNTGEIKSQTVFMGDFPMMTEKGTFI
                     INGTERVVVSQLVRSPGVYFDENIDKSTEKTLHSVKVIPGRGAWLEFDVDKRDTVGVR
                     IDRKRRQPVTVLLKALGWTTEQIRERFGFSEIMMGTLEKDNTAGTDEALLDIYRKLRP
                     GEPPTKESAQTLLENLFFKDKRYDLARVGRYKVNKKLGLHAGEPITSSTLTEEDIVAT
                     IEYLVRLHEGQTSMTVPGGVEVPVEIDDIDHFGNRRLRTVGELIQNQIRVGLSRMERV
                     VRERMTTQDVEAITPQTLINIRPVVAAIKEFFGTSQLSQFMDQNNPLSGLTHKRRLSA
                     LGPGGLSRERAGLEVRDVHSSHYGRMCPIETPEGPNIGLIGSLSVYARVNPFGFIETP
                     YRKATDGVVSDEIHYLTADEEDRHVVAQANSPIDDDGRFTEPRVLVRRKGGEVEYVSS
                     AEVDYMDVSPRQMVSVATAMIPFLEHDDANRALMGANMQRQAVPLVRSEAPLVGTGME
                     LRAAIDAGDVVVNDKSGVVEEVSADYITVMADDGTRHSYQLRKFARSNHGTCANQRPI
                     VDAGDRVEAGQVIADGPCTDNGEMALGKNLLVAIMPWEGHNYEDAIILSNRLVEEDVL
                     TSIHIEEHEIDARDTKLGAEEITRDIPNVSDEVLADLDERGIVRIGAEVRDGDILVGK
                     VTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGESGKVIGVRMFSRDEDDELPA
                     GVNELVRVYVAQKRKISDGDKLAGRHGNKGVIGKILAVEDMPFLPDGTPVDIILNTHG
                     VPRRMNIGQILETHLGWCAKSGWKVDGRPDWAAKLPEELLEAQAETIVATPVFDGARE
                     EELQGLL"
BASE COUNT         2959 a         5931 c         6110 g         2989 t
ORIGIN      
        1 cgatgacgac gagttcaggt ggtgcgccgg tcgtcacacc ggagccgccg acgaattccg
       61 ggcctccgtc gattaccgac aacggccctg gcccgatcag gacgatgctc aacgacgcgt
      121 tcagcatggt tcccggcaag ccgcagacgg ccagcgggag caactcttcc gcgacgtcgc
      181 ccaagcagcc gaaggcgtgg aagccggggg atgtgctgcg gcgcgtcacc gcgcccctcc
      241 gggaggcgat tgacgggctg accaagccgc gaaccacgac gccccccggc ggcgacgtgc
      301 agggtccccc gccgtccgac aacggggagg ggcaggccgc ggcttaggct gcggcgcctg
      361 gcttcaggta cgtcaccagg ctgacgtcga tgctctcgtc gatgaagtag tactgggcac
      421 ccttgaggta cttcatgtat cggtggtagt tctcttcatc ggtcgccgcg accgctgcgt
      481 ccttgttgcg ctccagcgcg tcggcccaca gcccgagcgt cttgatgtag tgcgtccgca
      541 gcgactgggg ctccggcacc acgaacccgg ctttctcgcc gtgctccacc atcatcttcg
      601 tagtcggcaa ccggccgccg gggaagatct cggtgatgat gaacttgacg aaccgggcca
      661 gctcgaacgt cagcttcttg cccttgtctg ccagatcgaa cgggtggaat ccgacgctgc
      721 tctggattgt catccggccg tcgtcgggca ggatgtcgaa gcaggtcttg aagaagtcgt
      781 cgtaccgctc gaagccgaag tgctcgaacg cctcgatcga cacgatccgg tcgaccggct
      841 cgtggaactg ttcccagccc tccagccgga cgtcgaattt gcggtcgctg tctgccttgg
      901 cgagcagctt gtcgcagtag gcctgctggt tcttcgacag agtcaagccg atgacgttga
      961 cgtcgtattt ctccatcgcc cgctgcaacg tcaggcccca cccgcagccg acctccaaca
     1021 gagtcattcc cggcttcagg tcgagctttt cgaggttcag atctacgtcg gcgatctgcg
     1081 cctccgacag cgtgacatcg ggaccggtga aatacgcgca gctgtacttg cgcgtcgggt
     1141 cctggaacac gccgaagaag tcgtcggaca ggtcgtagtg ggcctggatg tcctcgaagt
     1201 gcggctgcat gtcgtcgccg actttcgtaa acctcttgtc agccatggtg gttctcctat
     1261 ttctccagcg tgaactggtt gacgtcgatg ttcccggcgc ggaagccttt ggcgcagccg
     1321 gtgaggtagt gcatgtagcg gtcgtagacc tcttccgact gggtcgcgat cgcctcgtcg
     1381 cgacgagact ccagcgtctc ggcccacacg tccaatgtct tggcgtagtg cggctgcaga
     1441 gactgaatgc gcgtgacggt gaagccggcg tcggtcgccc gctcttgaac catctcgata
     1501 gtcggcaact gcccgcccgg gaagatctcg gtcatgatga acttcacgaa gcgggccagt
     1561 tcgaacgtca gggggacgcc gcggtcgagc gcctgcttca tggtcagcga cgtgatcgtg
     1621 tgcagcagca tcacgccgtc gtccggtagc acctcgtggg cggtcttgaa gaagtcgtcg
     1681 tagcggtcct ggccgaagtg ctcgaacgcg ccgatcgaca cgatccggtc gacgggttcg
     1741 tcgaactgct cccaaccgtg cagtagcacc cgcttcgagc gtgggctggg agagttgtcg
     1801 aacagcttct gtaggtgcgc ctgctggttc ttcgacaacg tcaggccgac cacattgacg
     1861 tcgtagcgct cgatcgctcg cagcatcgtc gcgccccagc cgcagccgat atcgagcagc
     1921 gtcatcccgg ggtcgaggcg gagcttgccc agtgacaagt cgatcttggc catctgcgcc
     1981 tcttcgagcg acatgtcgtc gcgctcgaag aacgcgcaac tgtacgtctg cgtcggatcg
     2041 aggaacaggc ggaagaagtc gtcggacagg tcgtagtgcg cctgtacgtc cgcaaagtgt
     2101 ggcttcagac cagccctacc cgccgatttc gtcatgattg ggtgcccttc cggctgcaga
     2161 gacgcatgtg cagtgatccc cagcaccgca cgcactcgat cagcgatgct agtcgatgcg
     2221 ggtctgcctt ccgaattcat cgggggcgcg ccgctggtca acgagctgcc gcgaacgtcg
     2281 tcttcagctc gccgacgatg tcgtcccaca gctcctcggg cagctcgtgg cccatgccgt
     2341 cgtacatcac cagccgcgcc ccggggatcg cgcgggccac cgcccggccg ccgaccggcc
     2401 gcatcagctt gtcggccttg ccgtggatca cgaccgtcgg cgcctcgatc tggcggttgt
     2461 agcgcagcag gctgccggtg cccaggatcg cgccgaagtg cctggcgaag ccggccggat
     2521 agtacgagcg gtcatagcct tcgatcgcct cggcgcggat cgcgtcatcg ctgcgcgggt
     2581 agccggggct gccgatgatc ttgttggccc tgatcgcgtt ctcgacgatc gcctcgcggg
     2641 aggaatcctt cggcttctgc aggatggcca tcaggtgctt cggcccgggc ggcggcagca
     2701 gcggctggtt gttgctggag aagatgacgg ccagcgtgtc agtgcggggt ttgtgccgcg
     2761 cggcgaagat ctgcgcgatc atcccgccca tcgacgcgcc gaccacgtgg gctttgtcga
     2821 cgtcgaggtg gttcagcagc gcggcggcgt cgtcggccat gtgctcgagg gtgtagacgg
     2881 acgggttgcg cgcaccgaag aagtgccgca ccatcttggg cggcaacgac ccggggtgac
     2941 ggtggccctg cagcttggtg gacagtccga cgtcgcggtt gtcgtagcgg atcacccgca
     3001 ggccctggtc gacgagcctc tcgcagaatc ccgtgcgcca caggacaagt tgcgcaccca
     3061 ggcccatgat cagcagcacg gccgggtggt tcgggtcgcc catgtcctcg tagtggatgt
     3121 cgatatcgcc ggagggggcg gtgccggtcc tcaggtccac tacgcgtcga cctcgtctgt
     3181 ttcatgctct tcgcgcgagc gctcatcgac gtgctcgcgg ctgacctcga ccatgaagtt
     3241 cgcgaagtag ccggtgagct cggggtccga catcatctgc cacttcggcg ccagcagctt
     3301 catgtagcgc tcgacgtaga ggaactgctt gccgatcagc accagctcgc gcggcagctt
     3361 cacgtcgtag gcgtcggcca gcgtcgagag ctgcttgccg atctcggcgt agctcatgtc
     3421 gccgagcgac ttcacggtca gcggctgcgc gaacttctct aagtccttgg cggcttgcgc
     3481 ctcgggtttg acggtgccga ccgcgcccat caacacgacg atcttgccgg ccgcggcgtg
     3541 gtcctttttg accagcagcg cgtacaccag ttctcgcagc agccagcgtg tgcgcgggtc
     3601 gatgcggccc atgatcccga agtcgaagaa cacgatcttt ccgtcggggt cgacgtagag
     3661 gttgccggcg tgcaggtcgc cgtggaacag cccgtgtcgc aggccgccct cgaacacgct
     3721 gaacagcagc gccttgacga ggtcggtgcc gtcgaatccg gccttgcgga ttgcggccgc
     3781 gtcgtcgatg cggatgccgt gcacgtgctc catcgtcagc acgcgagcgc tggtgtggtc
     3841 ccagtacacc tgcggcacgc ggatgttctt gcccagcggc gacgcgtgca tgtgcgcgac
     3901 ccaggcgtcc atcgactggg cctcgagacg gaagtcgagt tcctccgcga gattgtcggc
     3961 gaagtcggcg acgacgtcct gtgccgagag tcggcgtccc agcttggcca gctctactat
     4021 ttgcgcgccg cgcttgagga tttgcagatc ggcggcgacg cggcggcgga taccggggcg
     4081 ctggatcttg acgacgacct cctcgccggt gtgcagcgtg gcgtagtgca cctgcgcgat
     4141 cgacgcggat gcgaacggtt ggtcgtccca cttggcgaac agctctgcgg ggtccttgcc
     4201 gagctcctcg acgaacagct tgtgcacttc gtcgggatcg gccggcggca cggcgtcgag
     4261 aaggctgcgg aactcgcggc tgagcggttc accgaacgcg cccgggctgg acgcgatgat
     4321 ctgtccgaat ttcacgtagg tcggcccgag gtcggcgaag acgtgcggaa cctcggtgac
     4381 gagtttctgt tgccagcgcc ctttgttgaa aaggttgccg gcgacccggc cggcggctct
     4441 ggttacgtgc cagccggtcg caccgatacg cgcggcctct gtgaaaagcg gcacactgtc
     4501 gagcttggca acctcgcgat gcttggtaga gctcatagag gcagtgtgcc aaaccttctt
     4561 agtgttctca taatctgcgg gttcaccgct cacccggtcc atcatgcgcc cgtctgggtg
     4621 cggacggcgg aattgtcgct gcaaggcggg cacttcgagt accgccctcc tccggggtga
     4681 caggtgtggc tgaagtgaca atcgttacca cacgagcctc aggtgtcaca gtttcccctt
     4741 cgaggacggg atacgttcgt gcccgactct gagcgacaat tcgggcgact agccggcggg
     4801 cctccacggc ttgagccagt cggtctccgg ccagccttca agcccggcca ccaactcgta
     4861 catcgtcgcg ccgtcgatcg attcgcggat gacgtcggcc tgacctgagt gtcgggcgag
     4921 ctcgttgatc aggtggtgga tcacccagcg cacggaccac gccttcagat ccttcgggaa
     4981 ccacggcgcg tcagtcggca ccggaaccgg tgcgtcgagg tcggcggcgt cgatcagccg
     5041 cagcgtctcg gcgttctgcg cggcgaacgc gtccagtatc tgctcgagcg tctggtcgtc
     5101 gcgcatcacg aactcgtcct gaaaagctgc cgcgcgttct tcgaatggcc gggagtcggg
     5161 tgccggcgca tcaggtgccg ccgcgacccg ctgcatccac gtctgctgga cgccggtcgc
     5221 gtgtttgatc acaccgccga tcgacatcgc gctggccgac ggcgtcgcgc gcgcctgttc
     5281 gtcggtcaag ccgtaggcga tggcgaagta ggcgtcctgg tggtaggcga cgaattcacg
     5341 cagcgcgcca cgttcgtcgg cgacgggcga ggcaagtgca gacatgtgcc ttctccttac
     5401 tgcgagtgga cgtaaaaatc acggatacaa gcgatttcgg cgccgtggtg gatcacttcg
     5461 cggttgatgt gcaggacgag tgtggacatc gggtactcgg cgtaaggtcc ctcggcaggc
     5521 ccgcgcgggt tggccagcgc agccgcatcg agccggtccc ccagttgcgt cgtcaagtcc
     5581 atgacttcag actgcctgcg ccgcgcggcc gtcaccaccg ccggcaaggg acagttccgc
     5641 cgccacgccg aagtgatcgc tcggccatcc gcccccacgc ggctcgttgc agaccacgtg
     5701 ggcggtcagc actcggcctg ccccgtcttc ggcgtgaacc ccgacgaaga tgtagtcgat
     5761 ccgttgatcg ggcacctgct ccgcggcggc gaacgggttt gcgttgctcc acgtgtagcc
     5821 cggttgaccc gggttggcga cttcgaaagc gtcgaaaaga tggaagcttc tttggtcgag
     5881 ggcgtgcagc cccttgatga accgcacttc ggtggattcg ggtctggcgt tgaagtcgcc
     5941 acagacaatc ggaggaaagt cgtaggagtg tgcgccgacg acgtcggcga cgtcgcggac
     6001 ctgctgttcg cgtacccatc cctcgtcgaa gcgccacgac aggtgcgtcg atgagaacga
     6061 cacgcgacgc ccgcacgcgt cggcctcgac ggttatcacc gcgcgcggct cgttgcccgt
     6121 cgacgcgtcc gtgagtcggt gacgcagaga tccgtgcacc ggcagccggc tgagcaccgc
     6181 gttcccgaat tcgccgtcgt ctctggtgcg cgctgcaccg aaggccgcag tcatctccgt
     6241 gcgctcggcg atccacgacg cctggcacag tctgctggtt cggaccactt cctgcagggc
     6301 caacaggtcg ggttggattt cattgatcca atcgacgacc gccaatcgcc ggtcttccca
     6361 cttgttgctg ccccacagat tcaacgtcat cacggcgagt gtggatttct ggcttccttg
     6421 cacagtcacc acgggatgtt cgcacgaacg cgcacgtaaa gtgtctggcg tgcacgtcat
     6481 ctccgcggaa tcgacggagc tgttcgcggg accgcctgat gcgccgctgc aactggtgcg
     6541 ggtgacgttc cgcgggcgca gggagccggt ccgcgtggac ggcgaccgca tcacgaccgc
     6601 gggtgagccg cggtattcgc agggcgttgc cgaagtgccc gtcgaggtcg tcgaccccgt
     6661 gccgggcgaa cagcgcgacg cgcgggtggt cgtcggcggg acgacgacgc cgttctcgtt
     6721 cgccgtggct gagccgggct ggacgatgtt catgatcagc cacttccact acgacccggt
     6781 gtggtggaac acccagggcg cctacaccag cgtctggacc gccgacccgc cagaccgcca
     6841 gatgcacggg ttcggcctcg tgcgcgcgca tttggagatg gcccgccgtg agccggagta
     6901 caagttcgtg ctggccgagg tcgactacct caagccatac tgggacacct atccggaaga
     6961 ccgcgccgac ctccgccgca tgatcgccga cggccgcgtc gaaatcatgg gcggcgccta
     7021 caacgagccg aacaccaacc tcaccagcgc cgagacgaca atccgaaact tcgtgcacgg
     7081 catgggattt cagcgcgacg tgcttggggc tgatcctgcg acggcatggc aactggacgt
     7141 attcggccac gacccgcagt tccccggcat cgcggccgac gccggcctga cgtccagttc
     7201 gtgggcccgc ggcccgcacc accaatgggg gccgatctcc agcgccggtg atccgcgccg
     7261 catgcagttc tccagcgagt tcgagtggat cgcgccgtcg gggcggggcc tgctcaccca
     7321 ctacatgcct gcgcactacg gggcggggtg gtctctggat tccgcgccga ccctcgccga
     7381 ggccgaggcc gccgcgttcc agctgttcgc gacgctgaaa accgttgcgc tgacgcgaaa
     7441 tgtgctgctg ccggtgggga cggattacac gccgccgaac aagtgggtga ccgacatcca
     7501 ccgcgactgg aacgcccgct acacctggcc gaagttcatc tgcgcgctgc cgcgcgagtt
     7561 cttcaccgcg gtgcgagcgg aactttccgc gcggggagcg cagccctccc cgcaaacccg
     7621 cgacatgaac ccgatctaca ccggcaagga cgtgtcgtac atcgacacca aacaggccaa
     7681 ccgggcggcg gagaacgccg tactggacgc cgagcggttc gcggtgttcg ccagcctgct
     7741 cggcggtgcg acgtacccgc acgccgcgct ggcgaaggcg tgggtggaac tggcgtacgg
     7801 cgcccatcac gacggcatca ccgggtcgga gtcagaccag gtgtacctcg acctgctgac
     7861 gggctggcgc gacgcgtggg agctcggcgt caccgcgcgc gacaacgcgc tggcgctgtt
     7921 gtccagcgtc gtcgggggat cggtggtggt atggaacaca ttggcgcaca acagaaccga
     7981 catcgtcact gcgcgtctgc cggagccggt tggtcccggg gtccgggtgt tcgacgtcac
     8041 cgacggcgtc gaggtgcctg cgttggtaga gcacgacggg cactcgatca gctggctggc
     8101 gcgcgacgtg ccgtcagtgg gttggcgggc gtaccggctt caggcctctc gtgccgcgat
     8161 aggctgggag ttgttgtccg gcaacgagat tgcgaacgag cactaccggc tgacggtgga
     8221 tgcggcgcgc ggcggcgcgg tgtcctcgct cgtcgacctc aaaagcgagc gccagctgat
     8281 cgctgacggc cgggtgggca acgaactggc ggtgtatgag gaatacccgg cgcacccgcg
     8341 ctccggcgaa ggcccgtggc acttggtgcc caagggcccg gtgatctcct cgtcggcgtt
     8401 tgaggccgag tcggtgcggg cataccgcgg atcgctggga cagcggattg tggtgcgcgg
     8461 ccgggttggt gaactgttgc gctacaccca gaccgtgaca ctgtgggacg gcgtcgcgcg
     8521 ggtcgactgc cagacgacgg tcgacgagtt caccggcgcc gaccgtctgg tgcggctgcg
     8581 ctggccgtgc ccggtgcccg gcgcgctgcc ggtgagcgag gtcggcgacg cggtgatcgg
     8641 ccgcggcttc gggctgatgc acgaccgcgg ttccgaagcc gccgtcgacg ccgcgcagca
     8701 tccctggacg ctcgacaacc cggcgtacgg ctggttcggg ttgtcctcgg ccgcacggat
     8761 tcgggtcggt gatcacctac gggcggtgtc ggtggccgag gtcatcacgc cgggggaagc
     8821 gggtgcctcg tcactggcgc gcgatctgat ggtggcgctg gcccgcgccg gtgtcacggc
     8881 cacctgcagc agcgctgcca actcgcggta cggcgcgctt gacgtcgact ccaatctgcc
     8941 ggacacccgg atcgcgctcg gcggccctga cgaaaattcc ttcactgcgg aggttttggc
     9001 cgctgccgac ccggtcttta ccgacgagct caagaggcag ctgtacgccg ccggcgccgc
     9061 ccgggtgtgg gtcgcggcca cggccccgct cgccgacaag tgggtgccgg acgctgacct
     9121 gcggggcgcc cgcgcgctgc cggtgctggt gatcgcgtca gcacgactcg gtgaggccgt
     9181 cgccgcagtt gtcgacgatc tcacggatgc cgagatcgtc gtaacacaag atgcaccaac
     9241 agaattggag tcattcgagt cgcggacggt cgcactgctc aatcgcggca tgcccggctt
     9301 cgcggtcgac tccgacggca cgctacacac ctcgctgatg cggtcctgca cgggctggcc
     9361 ctccggggtc tggatcgatc cgccgcggcg caccgcaccc gacgggtcga acttccaatt
     9421 gcagcactgg acccacacat tcgactacgc gttggtgtcg ggcgacggcg attggcggcg
     9481 cgccggtctg cccacccgca gcgccgagtt ctcccaccct ctgcgtgccg tagtcggcgg
     9541 tggcgaaacg gccggtggcc taccacattt cggttcgctg ttcgaggtcg aacccgccgg
     9601 tgcggtccag ctggccgcgt tgaaggctgc cgggaatccg ctcgccagcg ccagcgggca
     9661 gcacgtcgac ccgtcggagg gggtggcggt gcgcctcgtc gaaacctccg gggcttcgac
     9721 ggacgtcgcg atccggtccg ggctacgccg ggtcgcgtcg gcttcgcgcg tcgacctact
     9781 cgagcagccg aggctgcaga gcatggcgac cgacgggctg acgctgcacg gtttcgagat
     9841 cgcgaccttc ctcacgcgcc tcaatcagcc gaagctgctg gacgccgacc acgtgatgct
     9901 ggctccggat gctgaagcgg cgcaaccgct ttacgcgcga tactggctgc acaaccgcgg
     9961 gccggcgccg ttgggcgggc tgcccgcggt tgcgcatctt cacccgcagc aatcccccgc
    10021 aagcgggagg gacccccaca gcgccgaccc gaattcgcaa ctgttgcttc gacttaccgc
    10081 tgcgagcgac tgcagcgacg ctgcgttgca cggccgcgtg cggctggtgt gtcccgacgg
    10141 ctggaccgcg aacccggccg atttgccgtt cgtgttgccg cccggcgagc atctggagac
    10201 cgacatcgag ctggccgtcc cggcgaagac gccgcccggg ttgtacccgg tgcgcgccga
    10261 gctcgccatc accggaagcg acgcgccctc catgcctgcc gcgtggcggc aaggggtcga
    10321 ggacgtctgc gtcatgtccg tcggcgacac cgccgacctc gccgtgctgc gcctgatccg
    10381 cgaacccgag cccgtcgacg tcacctccgg gggctcggca cggctcgcgg tgacggtcgg
    10441 caccgacgcc cgcgcggatc tggccgtcga ggcgcacctg atcagtccgt ggggcacgtg
    10501 ggagtggatc ggtccggcgg ctgtcggcgc gacgttgccg gcgggaggaa gcgtcgaact
    10561 cggcttcgac gtgtcgccgc cggcgtgggt ggaaccgggg gagtggtgga cccttgtccg
    10621 ggtcgggtgc gcgggtcgcc tcgtgtactc gcccgcggtg aaggtgacgg tccggtgacc
    10681 gcgacggcgg caacggtcga cggtgtgccg atcaccgtgg acgaggtcga cgcgcgggag
    10741 gccgcgctgc gtgccggacc gatagcggcc acgctgcccc gcgcgggcac cagcgagggc
    10801 cgtcaactgc ggcgctggct gacccagctg ctggcgaccg aacgcgtggt gaccaccgag
    10861 gcggcagccc gcggattgac cgcgagcgtg gcgccgagcg aagacgagct gttgccggac
    10921 ataacggccc gcctcgagat cggaagtatc accgcggccg ctctggccga cccgctgggc
    10981 cgcgcgttgt tcgccgacgt cacgtctgcg gtcgacgtga ctggcgacga cgtcgacggc
    11041 taccaagccc gcaaccccgg gcgctttccc gactccaccg cagtcgtcgc gcacctgcgg
    11101 gcggccgcac gacgccgcgc cttccgggtg tggcttgacg cgcgccgggc cgcgctcgtc
    11161 gaactcgccg ccggctacga acaccccggc gacccgcgac aacccgacaa cactcaccgg
    11221 cactgatggc atcgctcact ctcgcgatcg acatcggagg aacaaaagtc gctgcgggac
    11281 tggtgaattc gagcggcggg ctggttcgca aggcgacatc gccaacccct cacggcgacg
    11341 cggaggcggt atgggccgcc gcgcggcaac tcgtcgtcga gatgacgtct ggcgacgccg
    11401 ttcgcggggt gggcatctcc tccgccgggc cggtcgacct tccggcgggc acggtcagcc
    11461 cgatcaacat cgccagttgg ggcgactttc cgatcgtcga gcgcgtcgcg gcgatggtgc
    11521 cgggtgtgcc ggtgcgcctc ggtggcgacg gcctgtgcat ggcgctcggc gagcaccggc
    11581 acggggccgg acgtggcgcg cagtttctcc tcggcatggt ggtgtcgacc ggcgtgggtg
    11641 ggggcctagt gctcgacggc gcgccgtatg acggcgccac cggcaacgcc gggcacgtcg
    11701 gtcatgtcgt cgtggacctc gacggcgagc ggtgtacgtg cggcggtcgc ggctgcgtcg
    11761 aaaccatcgc ggccggcccg cggatggccg cctgggcccg tgcgaatggg tggaccgcgg
    11821 gtcccgacgc cgacgcgaag gagctcgccg aggcggccat gtcaggaaat gcattggcgc
    11881 tcagtgcctt tcgccggggt gccgctgctg tcgcggcgat gatcgcgtcg gtggctgcgg
    11941 tctgcgacct ggacctcatc gtgatcggcg gtggggtggc gaagtcgggt gcgctgctgt
    12001 tcgacccggt gcgtgaggcg ctcggcacct acgcccaact ggatttcatc cgcggcatac
    12061 gggtggtgcc cgcggagctc ggcggtgacg ccgggctggt cggcgcggcc gcgctcgtcg
    12121 acgcctgagg gatgcccgcg ttttggctga tcggacccgg ttgcgctaac ctggggcggc
    12181 tccaccgaag accgtcggtc accggagaaa tccggttgaa ggtccgggac atgtcccggc
    12241 ggcccacgca ggcggacgag gcaaatttgt gcgccccgac cctctgcgtc ggggcgtctg
    12301 tcatttccgg ccagtcgacg atcctcctgg ggcgcccgat acccacccaa ggaggcatga
    12361 atggccaagg ccgacaaagc cgccgccgtc gccgacatcg ctgagcagtt caaggaagcg
    12421 accgcggcgc tcgtcaccga gtaccgcggt ctgacggtgt ccaacctcgc cgagctgcgc
    12481 aggtcgctcg gcggctccgc cacctacgcg gtcgccaaga acacgctcgt caagcgggcg
    12541 gcgtcggagg caggcatcga cggcctcgac gacctgttcg ccggcccgac cgcgatcgcg
    12601 ttcgtcaccg gcgagcccgt cgatgccgcg aaggcgctga agaagttcgc caaggatcac
    12661 aaggcgctgg tgatcaaggg cggctacatg gatggccacc cgctgaccgt ctcggaggtc
    12721 gagcgcatcg ccgacctcga gtcgcgcgag gtgctgctcg ccaagctcgc aggcgcgatg
    12781 aagggcaact tgaccaaggc cgccggcctg ttcaacgccc cggcatcaca ggtggcacgg
    12841 cttctcgccg cgctgcagca acaaaaagaa agccaaccgg ctaccgccga ataaccccac
    12901 atccagaaag gacccacacc catggccaaa atgtccactg acgagctgct cgacgcgttc
    12961 aaggagatga cgctgctgga gctctccgac ttcgtcaagg cgttcgagga gaccttcgag
    13021 gtcaccgctg cggcacccgt tgccgtcgcc gccgctcctg gcgctgccgc gggcgcgccc
    13081 gccgaggctg ccgaggagca gagcgagttc gacgtgatcc tcgagtcggc cggcgacagg
    13141 aagatcggcg tcatcaaggt cgtccgcgag atcgtctcgg gcctgggcct caaggaggcc
    13201 aaggacctcg tcgacagcgc cccgaagccg ctcctggaga aggtcgccaa ggacgccgcc
    13261 gaagacgcca agggcaagct cgaggccgcc ggcgccacgg tcagcgtcaa gtagttcatc
    13321 ggtcaggggc cgccccagtg atgggcggcc cttttcacgt cacaacctgg ctctataagt
    13381 aacggcaggt gctgtcgtag gctagagtga ccccagccac aaggcgggag gcgcgtggcg
    13441 gcacagcatt ggcggaagga tcaccgtggg catcggaatt caagttgagg ggctgaccaa
    13501 gtccttcgga tcccaggtca tctgggagga tgtcacgctc gatgtgcccc cgggcgaggt
    13561 cagcgtgctg ctgggcccgt cgggtacggg caaatcggtg ttcctgaagt cgctgatcgg
    13621 cctgctgcgc ccggagcgcg gcaagatcct catcgacggc acgaacatca tcgagagctc
    13681 cgccaaagag ctgtacgaga ttcgcaagct gttcggcgtg atgttccagg acggcgcgct
    13741 gttcggctcg atgaacctct acgacaacac cgccttcccg ctgcgcgagc acaccaagaa
    13801 gaaggaaggc gagatccgtg acatcgtcat ggagaagctc tcggtggtcg gtctcgacgg
    13861 cgacgagaag aagttccccg gtgagatctc cggcggtatg cgcaagcgcg ccggcctggc
    13921 ccgctcactg gtgctcgacc cgcagatcat cctctgcgac gagcccgact ccggtctgga
    13981 cccggtccgt acggcgtacc tcagccagct gctgatcgac atcaacgcgc agatcgacgc
    14041 cacgatcctg atcgtcacgc acaacatcaa catcgcccgc acggtgcccg acaacatggg
    14101 catgctgttc cgccgcaagc tggtcatgtt cgggccccgc gaagtcctgc tgaccagcga
    14161 cgagcccgtc gtgcgccagt tcctcaacgg tcgccggctc ggcccgatcg gcatgtcgga
    14221 ggagaaggac gaggccacca tggccgaaga gcaagccatg gcggatgccg gccatcacga
    14281 cggcggtgtc gaggagatcg agggcgtgcc gccccagatc atggcgacgc ccggcatgcc
    14341 ggagcgcaag gccgtcgaac gccgacgcgc ccgtgtgcgc cagatgatga gcaccctgcc
    14401 gccgggcgcg caggaagcga tccgcgacga cctcgagggc acgcacaagt accactcgac
    14461 ccagttcgcc agcatggacg acggccgcca tgaagacggc gacgaggccc ccaccggacg
    14521 gatcccggtc tccagggagc gctagccggg ccgatgagcg cttgcgcgaa gagcatctaa
    14581 gcaggcatgc ccgagcagta ggcgacgttc tgccggatct gtgagccgct ctgcggcatg
    14641 gtcgcgacgg ttgacgagct ggtttggctc ccctccgccg cggtgtcgat gacttcgcgg
    14701 actcaggaat taacgcaatt cagcgcatcg aagaccgtgt gaactcttga cggaaacgcg
    14761 ccgaggcgtc agtctgttgg gcagcatgtg cataggtagc agtacaggac gccagccagc
    14821 gcctttactg ccgcatgtgc gtggttgagg aatatgccgt cctgcggtac tgttggacgt
    14881 tgcgctggct gcctcctgcc cacctcacct gaatctgaca ctgtggtcca cgcttgagcc
    14941 ctcattgggc ttgcctgcgt gtctgagaac cggacgggtc gtcagccggt gtgaaccgac
    15001 gcagatatag cgacgaagat gccggtcagc ccggtgcttg tggtcgcatg aggtgctgga
    15061 aggatgcatc ttggcagtct ctcgccagag caaatcagca gtagctaccg ccaatagctc
    15121 cgtccccgga gcccccaatc gaatctcctt cgcaaagttg cgtgaaccgc tcgaggttcc
    15181 cggcctgctc gaggtgcaga ccgagtcctt ccagtggctg atcggctccg acgagtggcg
    15241 cgaaaaggcg atcgctcgcg gtgacaccgc gaacccggtt ggcggattgg aagaggtgct
    15301 ctccgagctg tccccgatcg aggacttctc gggacagatg tcgctgtcgt tctccgaccc
    15361 tcgcttcgac gaggtcaagg cgcccgtcaa cgagtgcaag gagaaggacc agacctacgc
    15421 ggccccgctg ttcgtcacgg ccgagttcat caacaacaac accggcgaga tcaagagcca
    15481 gacggtgttc atgggtgact tcccgatgat gaccgagaag ggcacgttca tcatcaacgg
    15541 caccgagcgt gtcgtcgtgt cccagctggt ccgctcgccc ggggtgtact tcgacgagaa
    15601 catcgacaag tccaccgaga agacgctgca ctccgtcaag gtgatccccg gccgcggcgc
    15661 gtggctggag ttcgacgtcg ataagcgcga caccgtcggc gtccgtatcg accgcaagcg
    15721 tcgccagccc gtcaccgtgc tgctcaaggc gctgggctgg accaccgagc agatccgcga
    15781 gcggttcggc ttctccgaga tcatgatggg cacgctggag aaggacaaca ccgccggcac
    15841 tgacgaggcg ctgctggaca tctaccgcaa gctgcgcccg ggcgaaccgc ccaccaagga
    15901 gtcggcgcag acgctgttgg agaacctgtt cttcaaggac aagcgctacg acctggcccg
    15961 cgtcggccgc tacaaggtga acaagaaact cggcctgcac gccggtgagc cgatcacttc
    16021 gtcgacgctg accgaagagg acatcgtcgc cacgatcgag tatctggtcc ggctgcacga
    16081 gggccagacc tcgatgacgg tgcccggcgg cgtcgaggtg ccggtggaga tcgacgacat
    16141 cgaccacttc ggcaaccgac ggctgcgtac ggtcggcgag ctgatccaga accagatccg
    16201 cgtcggcctg tcccgcatgg agcgcgttgt ccgcgagcga atgaccactc aggacgtcga
    16261 ggccatcacg ccgcagaccc tgatcaacat ccgtcccgtc gtggcggcga tcaaggagtt
    16321 cttcggcacc agccagctgt cgcagttcat ggaccagaac aacccgctgt cgggcctgac
    16381 ccacaagcgc cgcctttcgg cgctgggccc cggcggtctg tcccgtgagc gcgccggcct
    16441 ggaggtgcgc gacgtgcact cgtcccacta cggccggatg tgcccgatcg agacgccgga
    16501 aggcccgaac atcggcctga tcgggtcgct gtcggtgtac gcgcgggtga acccgttcgg
    16561 cttcattgag acgccgtacc gcaaggccac cgacggcgtc gtctccgacg agatccacta
    16621 cctgaccgcc gacgaggagg accgccacgt cgtggcgcag gccaactcgc cgatcgacga
    16681 cgacggccgg ttcaccgagc cgcgtgttct ggtgcgacgc aagggcggcg aggtcgagta
    16741 cgtcagctcg gccgaggtcg attacatgga cgtgtcgccg cgccagatgg tgtcggtggc
    16801 cacggccatg attccgttcc tcgagcacga cgacgccaac cgcgcgctga tgggcgccaa
    16861 catgcagcgc caggcggtgc cgctggtgcg cagcgaggcg ccgctcgtcg gcaccggcat
    16921 ggaactgcgc gccgcgatcg acgccggcga cgtcgtcgtc aacgacaagt cgggcgtcgt
    16981 cgaggaagtc tcggccgact acatcaccgt gatggccgac gacggcaccc ggcacagcta
    17041 ccagctgcgc aagttcgccc ggtccaacca cggcacctgc gccaaccagc gcccgatcgt
    17101 cgacgccggc gaccgggtgg aggccggcca ggtgatcgcc gacggtccgt gcaccgacaa
    17161 cggcgagatg gcgctgggca agaacctgct cgtcgcgatc atgccgtggg agggccacaa
    17221 ctacgaggac gcgatcatcc tgtccaaccg gctcgtcgag gaggacgtcc tcacctcgat
    17281 ccacatcgag gagcacgaga tcgacgcccg cgacaccaag ctgggcgccg aggagatcac
    17341 ccgcgacatc ccgaacgtgt ccgacgaggt gctcgccgac ctggacgagc gcggcatcgt
    17401 gcgcatcggc gcggaggtcc gtgacggcga catcctggtc ggcaaggtca ctccgaaggg
    17461 cgagaccgag ctgaccccgg aggagcggct gctgcgtgcg atcttcggcg agaaggcccg
    17521 cgaggtccgc gacacgtcgc tgaaggtgcc gcacggcgag tccggcaagg tcatcggcgt
    17581 ccggatgttc agccgcgacg aggacgacga actgcccgcc ggcgtcaacg agctggtccg
    17641 ggtctatgtc gcccagaagc gaaagatctc cgacggcgac aagctggccg gccgccacgg
    17701 caacaagggc gtgatcggca agatcctggc ggtcgaggac atgccgttcc tgccggacgg
    17761 caccccggtg gacatcatct tgaacaccca cggtgtgccg cgacggatga acatcggcca
    17821 gatcctggag acccacctgg gttggtgcgc gaagagcggc tggaaggtgg acggtaggcc
    17881 agactgggcg gcaaagctgc ccgaggagct gctggaagcg caggccgaga ccatcgtggc
    17941 cacgccggtg ttcgacggcg cccgcgagga ggagctgcag gggctgctg
//
LOCUS       DASWBF010000240         5830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_804592,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000240 DASWBF010000000
VERSION     DASWBF010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804592"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VF874_08760"
     CDS             <1..137
                     /locus_tag="VF874_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176169.1"
                     /translation="KIRLLTRIAFGFRSPEALIALAMLALGGHRPSLPGRTKHPRISQ
                     "
     gene            328..453
                     /locus_tag="VF874_08765"
     CDS             328..453
                     /locus_tag="VF874_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176170.1"
                     /translation="MLGPDQVGVLLEELPDYLASRGATSDYLDDILDCSLLVVRH"
     gene            601..1014
                     /locus_tag="VF874_08770"
                     /pseudo
     CDS             601..1014
                     /locus_tag="VF874_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            1131..2279
                     /locus_tag="VF874_08775"
     CDS             1131..2279
                     /locus_tag="VF874_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9176171.1"
                     /translation="MSEHIRLAKVSGFPVSVHWSVLVILWLFTWSLASTLPHAAPGHS
                     SGAYWLAGVIGAVVLLASLLAHELSHAVVARRAGIKVSGVTLWMLGGIASLGSEAKTP
                     KTEFRVAVSGPATSLILAAVFAGVAEGLHVFGVAPIVVGVAWWLSGINLLLGLFNLLP
                     GAPLDGGRILRAYLWRRHGDPVRAAVGAARAGRILGFTLIGLGLLELVAGALVGGVWM
                     VFIGWFLLTAARTEETQVLTRQSLAGVRVADAMTAHPHTAPGGISVEDFIHRYLLGDR
                     HSAYPVEGPDGSIMGLITLTQLRGVAPNARATTLVREAAIPLHQVPTAAPHEPLTALL
                     ERLAPKAGTRALVVDAGHVVGIITATDITRLIDVRGLDLPRPAAGHPH"
     gene            complement(2306..2488)
                     /locus_tag="VF874_08780"
                     /pseudo
     CDS             complement(2306..2488)
                     /locus_tag="VF874_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
     gene            2773..2886
                     /locus_tag="VF874_08785"
     CDS             2773..2886
                     /locus_tag="VF874_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX9176172.1"
                     /translation="MLTATLISLGVVFVAELGDKSQLITKRLAATGGGPLR"
     gene            2916..3995
                     /locus_tag="VF874_08790"
     CDS             2916..3995
                     /locus_tag="VF874_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9176173.1"
                     /translation="MCTEPHLHPGAAVSSTIRPEPAVALADPVTLMPVDAVTLTTLVD
                     NSTDLLLADHGPVRRHGLLAAAAATRLATNVLDTGETYDVPLAEHGFSMLVTVTRDGH
                     DHHLLFDAGMTPDGLCDNMRRIGLSPFDIELVVLSHGHSDHVTGLDGFIRAVGPSNLP
                     VVVHPGAFTKRRIAIPGLLELELPSPSRGALREGGLDVVERRQSSLLFDNSVLITGEV
                     DRTTDFEHGMPFHEALTDGAWAPDPLILDDQALIVHVAGHGLVVLTGCGHAGIVNIVR
                     YARRLTGIDTVAAVVGGFHLSGPAFEPIIQPTVDALAAIGAEAIIPAHCTGWRAAHAL
                     AQAMPHAYIPNSVGSRLELRNEQPT"
     gene            4655..5383
                     /locus_tag="VF874_08795"
     CDS             4655..5383
                     /locus_tag="VF874_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9176174.1"
                     /translation="MYESAAHIPATCDEELLCCLGPNYPALAGSDPDRVARIRDEVAY
                     GFSALTDVAHAVSIFGSARTRPDHSHYKLARALATRLGTLGFDIITGGGPGIMEAANR
                     GARDAGVRSIGLGIELAHEQGINPYVDLSLQFSHFFVRKLMFIRYASAFVVFPGGFGT
                     LDELFEALMLIQNAKIRHFPVVLAVSEHWSGLEQWIRARLLEPGMVSADDVKLLVLAD
                     DPNEIGDIIEHWRRRQLETYRPTG"
     gene            5547..>5830
                     /locus_tag="VF874_08800"
     CDS             5547..>5830
                     /locus_tag="VF874_08800"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9176175.1"
                     /translation="MTTRQSGFDVIERALRRHTFGTLSTLTEQGRPHATGVEYAVSAP
                     GAPLALYVTTRTTTRKVRNIQAHPDVPFVVPVPHRLIALFPPSAIQFQGSA"
BASE COUNT          987 a         1940 c         1910 g          993 t
ORIGIN      
        1 ccaagatccg actcctcacc cgcatcgcgt tcggcttccg ctcccccgag gcactcatag
       61 ccctggccat gctcgccctc ggcggccacc gccccagcct gccgggccga accaaacacc
      121 cacggatcag tcagtagagc cgtaaaacgg gcgtggtatc agcgcaaccg tgaagcgatc
      181 cgggaaaagg ggcggcggca gcaacgctcg ccaaatcgca agcgctccaa gcagcgcaac
      241 gccgaacggc tgaagtcgat tccggtcacg tgcaccggcc ggtggaccga tgaagagacg
      301 agagtcgttg cccgtgatga catcacgatg ctgggacccg accaggtcgg tgtcttgcta
      361 gaagagctgc cggactatct cgcctcgcgt ggtgcgacaa gcgactacct cgatgacatc
      421 ctcgactgct cactcctggt cgtccggcac tgaccgccgg acgacgtgcc acgaccgcgc
      481 ggggtgctat gcctatgggt ggaggcccaa tgaccctagg gccggttcga tcgtcggccc
      541 tacgtttgga agtgcgcggg aatcgaatcg gcccgcaatg ccgcctttta ggaggacatc
      601 atgaccaccg cccgtgacat catgcacacg ggggcgacct gtgtcggcga gcacgaaacg
      661 ctcaccgccg cggcccaaca catgcgcgat ctcggtgttg gtgccctgcc gatttgcgga
      721 gatgatgatc gcctgcacgg gatgatcacc gaccgcgaca ttgtcatcaa ggccatcgcc
      781 gccggcctag atctcgacac cagcaccgcc ggcgaactcg cccagggcag cacctaccac
      841 gtcgacgccg aagcgagtat tgaaggcatg ctcaccgtca tggaagaact cccggtccgc
      901 cgcctgcccg tgatcgagga gcaccgacta gtcgggatgg tcagcgaggc cgacatcgcg
      961 cggcatctac ccgaacatgc catcgcccag ttcgtcaagg cgatctgcgc tccccggccc
     1021 tcaccagcca ctgaccctgc ggtctcgaag cggcaatccc tgccgccgac acctacggac
     1081 catcgcttca tagcgaaggg cgtgtccggc aggatcattg gaggttcgac atgagcgagc
     1141 acatccggct ggcaaaggtg tccgggtttc cggtgagtgt ccactggagt gtgctggtga
     1201 tcttgtggtt gttcacctgg agcctggctt ccactctgcc ccacgcggcg ccgggccatt
     1261 cgtcgggggc ctactggctg gccggggtga ttggtgcggt ggtgctgctc gcgtcgctgc
     1321 tggcccacga actgtcgcat gcggtcgtgg cgcgccgcgc gggcatcaag gtgtcggggg
     1381 tgacgttgtg gatgctgggg ggcatcgcga gcctcggcag cgaagcgaag acaccgaaaa
     1441 cagaattccg ggtcgcggtg tcgggtccgg cgaccagcct gattctggcg gcggtctttg
     1501 ccggcgtggc cgagggccta cacgtgttcg gggtggcgcc gatcgtggtc ggtgtcgcgt
     1561 ggtggctgtc cggcatcaac ctgttgctgg ggctgttcaa tttgttgccg ggcgcgccgt
     1621 tggatggcgg ccgcattctg cgtgcctacc tgtggcgccg ccacggcgat ccggtacgcg
     1681 ccgcggttgg cgccgcccgc gccgggcgca tcctcgggtt caccctgatc ggactgggcc
     1741 tgctggagtt ggtggcgggg gcgctggtcg gcggcgtgtg gatggtgttc atcggctggt
     1801 tcctgctcac cgccgcccgc accgaggaga cccaggtatt gacccggcag tccctcgcgg
     1861 gtgtgcgcgt cgccgacgcg atgaccgccc acccgcacac agctccgggc gggatcagcg
     1921 tggaggactt catccaccgc tacctgctgg gcgaccgcca ctcggcctat ccggtcgagg
     1981 gccccgacgg gtcaatcatg ggactgatca ccctgacgca actacgcggt gtcgcaccca
     2041 acgcacgagc caccactttg gtgcgtgagg ccgccatccc actgcaccaa gtgcccaccg
     2101 cagcaccgca cgagccgctc accgcgctgc tggagcggct ggcaccgaaa gccggcaccc
     2161 gtgcgctggt cgtcgacgcc ggtcacgtcg tgggaatcat caccgcaacc gacatcacca
     2221 gactgatcga cgtgcgcggc ctcgacctgc cccgaccagc cgccggtcac ccccactgac
     2281 tcacagatac gtcacaggtg agggatcaac cgtcgcgcgc cagcaccgcc acctgctcca
     2341 gtgtgcccgg ctcttcaaaa agatgggtgg cccctgaggc caccgcgatg tgacactcgg
     2401 ctgggatcgc ggactgcgcc cggcgattga gttcgagaac catcttgtcg cgcgcgccga
     2461 cgatcaacag cgtcggcgcc ataatcgccc gagtgtgggc tcaattgtgg tcgcgaccgg
     2521 aaccgggccg gacacggccg gcagtgtcgg tcctgtggag gcggcggagc gggtgcggcg
     2581 ctgggcggtg ggccgtggtc aggccggcgt tcgccgtggg taagcgaagg ggagtagccc
     2641 acaatcgtcg tgtcgacacg ctggcgcaca cgcgcccggc cggcgaaccg cggaagcggt
     2701 gggcgagacc ttcgaccgga tgtccggtga ccgcgcgatt gcggtgccgg gcgatgcgtc
     2761 gaaaggtcgg acatgcttac cgccacactc atcagtctcg gtgtggtctt cgtcgccgag
     2821 ctcggtgaca agtcgcagtt gatcacgaaa cgtctcgcgg cgacgggtgg cgggccgtta
     2881 cggtaaaccg gacatccgac tgaaagagac aagtgatgtg caccgaacct caccttcatc
     2941 ccggcgcggc ggtctcctcg acgatccggc ccgaacccgc tgtcgcattg gcggatccgg
     3001 tgacgctgat gccggtcgat gcggtcacac tgaccacgct ggtcgataac agcaccgatc
     3061 tgctgttggc ggaccacggg ccggtccgcc ggcacggcct gctggccgcc gccgccgcga
     3121 cgcggctggc caccaacgtg ctcgacaccg gcgaaaccta cgacgttccc ctggccgagc
     3181 atgggttttc gatgctggtc acggtgaccc gcgacggcca cgaccaccac ctgctctttg
     3241 acgcgggtat gacgcccgat ggtctgtgcg acaacatgcg tcggatcggg ttgtccccgt
     3301 tcgatattga gctggttgtg ctcagtcacg ggcattccga ccacgtcacc ggcctggacg
     3361 gattcatccg cgcggtcggc ccgtccaatc tgccggtggt cgtgcacccc ggtgctttta
     3421 ccaagcggcg catcgcaatt ccgggtctgc tggaactcga acttccctca ccgagccgtg
     3481 gcgcgctgcg ggaagggggc ctcgacgtcg tcgaacgccg ccaatcgtcg ctgctgttcg
     3541 acaattcggt gctgatcacc ggcgaggtcg accgcaccac cgacttcgaa cacggcatgc
     3601 catttcatga ggcgctcacc gacggcgcct gggcgcccga cccgctgatc ctcgacgacc
     3661 aggcgctgat cgtgcacgtc gccggtcacg ggctggtcgt gctgaccgga tgcgggcacg
     3721 ccgggatcgt caacatcgtc cgctacgcgc gtcgcctcac cggtatcgac accgtggccg
     3781 cggtcgtcgg cggtttccac ctcagcggtc cggcgttcga accgatcatt caacccaccg
     3841 tcgacgcatt ggcggcaatc ggcgccgagg ccatcatccc ggcccactgc accggatggc
     3901 gcgccgcaca cgccctcgcc caagccatgc cgcatgccta catccccaac agtgtcggct
     3961 cgcgcctgga actgcgtaac gaacagccca cctgacaccg gccgcggaca tggccggcaa
     4021 ccgccggaca ccctgcccca ttggagtgac ggacatcggc ccgggcattc cgaactgacc
     4081 gaagaccctc gcggttgccg gtacttgtaa tgcggtgccg cgcaacgtgt gccgaatagg
     4141 gacggtcgcc ctgtggggca tggaggacgg gctgagcatc gaggaggtcg ccgaccaaat
     4201 gggcaaatcg gtcgagatgg ttcaggactc ccccaggttg gcctcgacgg gcggcgcagc
     4261 ggacgtcgtc caacgtcgat ccgccgccgg acagttcgtc ttcgcggctt atcgtcggct
     4321 agaaatcctg gcttgatcgc acgccgatcg cacgccagag cgcgctaact gtagcgacgt
     4381 gcgataactc ccgctaaccc ggctggattt gactcagctg gggcgagatg ccggaccggt
     4441 caagccgccc cgggtggcgg ggtcgaggcg agtgtcagat gcgggctgat gaccgctcgc
     4501 accgaataca ggtcgtgcta tcaacgcggc taggcccgtt ggtatccggc caccatcaag
     4561 gctgtggtgg ctagcgatgc cgcggcgcta taacgaggca gcggccgaat ggccggcgga
     4621 ccatcggcgt agtcgccgtg ggacgatcgt gcgaatgtat gagtccgcag cgcacattcc
     4681 cgccacctgc gatgaggagc tgctctgctg cctggggccg aactatcccg cgctggcggg
     4741 cagcgatccc gaccgggtgg cccggatccg cgatgaggtc gcgtacggat tttcggcgct
     4801 aaccgacgta gcgcacgccg tgtcgatctt tggctcggcg cgcacgcggc cggatcactc
     4861 gcactacaag ctggcgcgcg cgctggccac acggctgggc acattgggtt tcgacatcat
     4921 caccggcggc ggtcctggga tcatggaggc cgccaaccgc ggcgcccgcg acgcgggggt
     4981 gcgctcgatc ggcctcggta tcgagctggc ccacgagcag ggcataaatc cgtacgtcga
     5041 cctctcgctt cagttcagtc actttttcgt ccgcaaactg atgttcatcc gctacgcgtc
     5101 ggcgttcgtg gtgtttccgg gcggcttcgg aacgctcgat gagctcttcg aggcgctgat
     5161 gctcatacag aacgcgaaga tacggcactt tccggtcgtg cttgcggtgt cggagcactg
     5221 gagcggactg gagcagtgga tccgcgcccg gctgcttgag ccggggatgg tttcggcgga
     5281 tgacgtcaag ttgctcgtgc tcgccgacga tcccaacgag attggcgaca tcatcgagca
     5341 ttggcggcgt cgccagctgg agacctaccg cccaaccgga tgagtggtgt tgatgacagc
     5401 agacgaggat cgggtacgcc cgaaccgcaa ggcctgccca gaagcgacgg gtgcccgtca
     5461 ccggcagtcg gtcgccataa tgacgggttc gggggacatc ggcgacatca cagtcgcgca
     5521 caagaaacca gatagcagga atagcaatga ctactcggca gagcggcttc gacgttattg
     5581 agcgtgcatt gcgccgtcac acgttcggca ccctgtcgac gttgaccgag cagggacgcc
     5641 cgcatgcgac cggggtggaa tacgccgtat ccgcgcccgg cgcaccgctc gccctctatg
     5701 tgacgaccag aacgacgacg cggaaggtgc ggaacatcca ggcgcatcct gatgttcctt
     5761 tcgttgttcc ggtgccccac cgcctaatcg cgttatttcc accgagcgcc attcagttcc
     5821 aaggctcggc
//
LOCUS       DASWBF010000241         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1006407,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000241 DASWBF010000000
VERSION     DASWBF010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006407"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF874_08805"
     CDS             complement(<1..327)
                     /locus_tag="VF874_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9176176.1"
                     /translation="MDSTMQDFPLTITGILRYGTGWHVDRKVITATDDGYREASYGAV
                     GERVAQLAHGLRGLGITGDERVATFMWNNQEHLEVYYAAPCMGAVLHTLNIRLPADQL
                     AYVAGQA"
     gene            466..1572
                     /locus_tag="VF874_08810"
     CDS             466..1572
                     /locus_tag="VF874_08810"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HEX9176177.1"
                     /translation="MKTKAAVLWGINQKWEIEEIDLDPPKEDEVLVKMTASGLCHSDY
                     HLVTGDLPAPLPLIGGHEGAGVVVEVGPGVKEVAEGDHVVMSFLPACGRCKPCARGMS
                     NLCDLGAYLLTGARLDGTFRFHARGRDVGQFLLLGTFSEYAVVPTASVIKLDDRAPLD
                     KAALLGCGVTTGYGSAVRTGDVQPGDTVVVMGIGGVGVNAIQGARIAGARNIVAIDPV
                     DYKLKRSRDFGATHVAASVDDAIPMVKDLTWGEMANVCILTTDVAEGPYVAQALGLVG
                     KRGRAVITAIGRATDTTVNMSLFELTMWEKQVRGGLFGSSNPRSDIFELLSLYESGQL
                     KLDELVTCEYTLDDVNQGYDDMLSGQNLRGLIRY"
     gene            1775..2406
                     /locus_tag="VF874_08815"
                     /pseudo
     CDS             1775..2406
                     /locus_tag="VF874_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
BASE COUNT          401 a          781 c          823 g          401 t
ORIGIN      
        1 ggcctgcccg gcgacgtagg cgagctggtc ggcgggcaag cggatgttca gcgtgtgcag
       61 gaccgccccc atgcacggcg cggcgtagta gacctccagg tgctcctggt tgttccacat
      121 gaacgtggcc acccgctcgt caccggtgat ccccaggcca cgcaggccgt gcgccaactg
      181 cgcgacccgc tcgcccacgg cgccgtagct ggcctcacgg tagccgtcgt cggtggcggt
      241 gatcaccttg cggtccacgt gccaacccgt gccgtaccgc aggattccgg tgatcgtcag
      301 cgggaaatcc tgcatggtgc tgtccactgc cacccccatc tgctttctgc tcttcgcgca
      361 agcggctcat cgctgcattc gtcttctgtc tcgatgtcca tcttggccca ccgccagcct
      421 tcgaaggcaa taatcgacgc acgactgaca ggaagggcac aggggatgaa aaccaaggcg
      481 gcggtgctgt ggggaatcaa ccagaagtgg gagatcgagg agatcgacct cgatccgccg
      541 aaagaggacg aggtcctggt caagatgacc gccagcgggc tgtgtcactc ggactatcac
      601 ctggtgaccg gcgacctacc ggcgccgctg ccgctaatcg gcgggcacga gggcgccggt
      661 gtcgtggtgg aagtcggccc gggtgtcaag gaggtcgccg agggagacca cgtggtgatg
      721 agcttcctgc cggcctgcgg ccgctgtaag ccctgcgccc gcggcatgag caacctctgt
      781 gatctcggcg cctacctgct gacgggggct cggctcgacg gcacgttccg gttccacgcc
      841 cgcggtcgag atgtcggcca gttcctgctg ctgggaacgt tctcggagta cgccgtggtg
      901 ccgacggcct cggtgatcaa gctcgacgac cgcgcgccac tcgacaaggc ggcgctgctg
      961 ggctgcggcg tgacgacggg atacggcagc gcggttcgca cgggagacgt gcagccaggc
     1021 gacacggtgg tcgtgatggg catcggcggc gtcggcgtca acgcgatcca gggcgccagg
     1081 atcgccgggg cgcgcaacat cgtcgcgatc gatccggtcg actacaagct caagcggtcc
     1141 cgcgacttcg gcgcgacaca cgtcgccgcg tcggtcgacg atgcgattcc aatggtcaag
     1201 gacctgactt ggggcgagat ggcgaacgtc tgcatcctga ccaccgacgt ggccgaaggc
     1261 ccctacgtgg ctcaggcgct gggcctggtc ggcaagcggg gtcgcgccgt gatcactgcg
     1321 atcggacgcg cgacggacac aacggtcaac atgtcgctgt tcgagctgac gatgtgggaa
     1381 aaacaggtcc gcggcggtct tttcggctcg tcgaacccgc gcagcgacat cttcgagctg
     1441 ctgtcgttgt acgaaagcgg tcagctcaag ctcgacgagc tggtcacctg cgagtacacg
     1501 ctcgacgacg tgaaccaggg ctacgacgac atgctcagcg ggcagaacct gcgcggtctg
     1561 atccgctact aatcggctgc accgccgacc ccgccgacga cgaggtcgac ggcgctggcc
     1621 gcgcgaaggg tatgagcggg gcaccgtcaa cttgagcaat ccgagggcga ttttcgccgg
     1681 ttcgcctccg cgattcgcca ggtcgcgtgt gacgtatgag gcttatgtgg ttagcggaac
     1741 gtccacacga cctcaagttg acagtgccct cgcgacgccg ggccacactc cggaatccat
     1801 ctgcatcgtg gtctacgagc agcccgacga cgaggtgccc tacggcgtgc tcaccggtga
     1861 cacattgttc gtcggcgacg tggggcgccc ggatcttctg gcggcgtcgg gcctgtcggc
     1921 cgacaccctc gcccgtgcgc tgtatgggtc gcttcacgac aaactgctca cgctgcccaa
     1981 cgcgacgcgg gtgtttccgg cgcatggcgc cgggtcggcg tgcggcaagc agctgtccag
     2041 cgagacgagc tcgacgatcg gcgagcagcg ccagatcaac tatgcgctgc agacgatgga
     2101 cgtggatcgg ttcgtcgccg cggtcaccga gggacagccg gtgcggccgc actacttcga
     2161 gttcgacgcg caccgcaacc gggagcttag gccgctgctc gacgaacagg caccgggctt
     2221 gctcgacatc gacgaggtct gcaaccacgc cgacgcgggc gcgatcctgc tcgacggccg
     2281 ggagccggcc gactatgcgt ccggtcatct gcggggcgcg gtgaacgtcg gcttgcaggg
     2341 gcggttcgcc gagtgggtcg gcaatgtctt gtcaccggac cgcgacatcg tcctggtcgg
     2401 cgatcc
//
LOCUS       DASWBF010000242         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_83746, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000242 DASWBF010000000
VERSION     DASWBF010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83746"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VF874_08820"
     CDS             <1..500
                     /locus_tag="VF874_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX9176178.1"
                     /translation="TALRQKWNNSGEILMWIPLPLLGIPPWIVFLSFSVNLIYQFWIH
                     TERIGKLWRPVEFVLNTPSHHRVHHGMDPEYLDKNYGGILIIWDRMFGSFQPELFRPH
                     YGLTKRVDTFNIWKLQTYEYVAIAHDVRSAARWRDRLGYIFGPPGWEPRIERQHEVPA
                     STAAA"
     gene            541..1050
                     /locus_tag="VF874_08825"
     CDS             541..1050
                     /locus_tag="VF874_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HEX9176179.1"
                     /translation="MDVKEVLLPGVGLRYEFDIRDGDRVGVIARRSGGFEVVIYARDD
                     PDEARPLFRLTEEEAEALAQILGAPRIAERFADLTREVPGLNAGQVEIGRDSPFVDRP
                     LGDTRARTRTGASIVAIVRDDEVLASPGPAEVLHEGDVLVVIGTEEGIAGVEHIVDKG
                     DVAGPPAGG"
     gene            1053..2201
                     /locus_tag="VF874_08830"
     CDS             1053..2201
                     /locus_tag="VF874_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9176180.1"
                     /translation="MQISSTLLLELGVILAVLTVLGAAARRYALSPIPLYLVAGLALG
                     EGGIIPVPAAGAFVVSSASIGVVLLLLTLGLDFSIGEFASSLRRHLPSAWVDLILNAV
                     PGAVAGWLLGLGPVGILAMAGITYISSSGVIARLLNDLRRLGNRETPAVLSVLVLEDF
                     AMAAYLPLLAVLAAGGSWLGALLGMGIAVAALVAAFTASYRWGHHVGRLVAHPDSEQL
                     LLRVLALTLIVAALAEFVHASAAVGAFLVGLTLTGDTADRARKVLSPLRDLFAAIFFL
                     AIGFSVDPKELVPMLPAALALAAVTAATKVATGIYAARREGVARRGQLRAGTALIARG
                     EFSLVIIGLVGETIEAVASVATSYVLILAIVGPMLTRFVGAPPRVQAS"
     gene            2198..>2862
                     /locus_tag="VF874_08835"
     CDS             2198..>2862
                     /locus_tag="VF874_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9176181.1"
                     /translation="MTDQFVRLRDGRQLGFTEYGDPAGFPVVNCHGGLACRLDVAAAD
                     GVARDVSIRLISADRPGIGLSDPKPGRTVLDWAADVSELAEHLGLQRFSVMGWSMGGP
                     YAAAVGYGLRPHVNRVALIASAVPLGEPGAFEQLPQMDRAYTRLSQRSPWMARQCFRA
                     MAFVAGVAPSLYGRLAARDLGPADGSVIRNEGFGTFARMSREALRRPSGVVEEYRAWM
                     RPWG"
BASE COUNT          389 a          950 c         1003 g          520 t
ORIGIN      
        1 cgaccgcgct gcgccagaag tggaacaaca gcggcgagat tttgatgtgg attccgttgc
       61 cgctgttggg tattccgccg tggatcgtgt tcctgagctt ctcggtcaac ctgatctacc
      121 agttctggat ccacaccgag cgcatcggaa agctgtggcg acccgtcgag ttcgtcctca
      181 acacaccgtc gcaccaccgg gtgcaccacg gcatggatcc ggagtacctc gacaagaact
      241 acggcggcat cctgattatc tgggaccgca tgttcggctc gttccagccc gagctcttcc
      301 ggccgcacta cgggttgacc aagcgggtcg acacattcaa catctggaag ctgcagacct
      361 acgaatacgt cgcaatcgcg cacgacgtcc ggtcggccgc ccgctggcgc gaccggctcg
      421 gctacatctt cgggccgccc gggtgggagc cacgcatcga acggcaacat gaggtccccg
      481 catcgaccgc tgcggcgtaa cttctcgcac ggcgccgcta cgtagtcaag gattggctgc
      541 atggacgtca aagaggtgct actgccgggt gtcggcctgc gatacgaatt cgatatccgc
      601 gacggggacc gcgtcggcgt gatcgcccgg cggtcgggtg gtttcgaggt cgtgatctac
      661 gcccgcgacg accccgatga ggctcgaccg ctcttccgcc tcaccgaaga ggaggccgaa
      721 gcgctcgcgc agattctcgg ggcgccgcgg atcgccgaac gcttcgccga cctcacccgc
      781 gaagtgcccg ggctcaacgc cggacaggtc gagatcggcc gggacagccc gttcgtcgac
      841 cggccgctgg gcgatacccg cgcacgcacc cgaaccggcg cgtcgatcgt cgcgatcgtg
      901 cgcgacgacg aagtgctcgc gtcgccgggt cccgccgagg tcctgcacga aggcgacgtt
      961 ctggtagtga tcggcacaga ggagggcatc gcaggggtcg agcacatcgt cgacaagggc
     1021 gacgtggccg gcccgcccgc cggaggctga cagtgcagat ttcttcgacg ctgcttctgg
     1081 agctcggcgt catcctcgcc gtgctcacag tgctcggagc ggccgcgcgc cgctacgcgc
     1141 tgtccccgat accgctgtat ctggtggccg gcctcgcgct cggcgagggc ggcatcattc
     1201 ccgttccggc agccggggct ttcgtggtgt cgagcgcgtc gatcggcgtc gtgctgttgc
     1261 tgctgacgct cgggctggat ttctcgattg gcgagttcgc gagcagcctg cggcggcacc
     1321 tgccgtcggc ctgggtggac ctgatcttga acgcggtccc gggcgcggtg gcagggtggc
     1381 tgctgggcct gggccccgtc gggattctcg cgatggccgg catcacgtac atctcgtcgt
     1441 cgggtgtgat cgcgcggtta ctcaacgacc tgcgaaggct cggcaaccgc gagacacccg
     1501 ccgtgctgtc ggtcctcgtg ctcgaggact tcgcgatggc cgcgtacctg ccgctgctcg
     1561 cggtgctcgc ggcgggcggc tcatggctgg gcgcgcttct gggcatgggt atcgcggtcg
     1621 cggcgctcgt cgcggcgttc accgcgtcgt accgctgggg ccaccacgtc ggtcgccttg
     1681 tcgcacaccc ggattcggag cagctgctac ttcgggttct cgccttgacg ttgatcgtcg
     1741 cggcgctcgc ggagttcgtg cacgcctcgg ccgcggtcgg ggcgttcctt gtcggcctga
     1801 ctctcaccgg tgacacagcc gaccgtgccc gcaaggtgct cagcccgctg cgcgacctgt
     1861 tcgccgcgat cttctttctg gcgatcggat tctcggtcga tccgaaggag cttgtgccga
     1921 tgctgcctgc ggcgctggca cttgcggccg tcaccgccgc aaccaaggtg gccaccggga
     1981 tctacgccgc gcggcgcgaa ggggtggcga ggcgtggtca gctgcgcgcc gggacggcgt
     2041 tgatcgcgcg cggcgaattc tctctggtga tcatcggctt ggtcggggaa accatcgaag
     2101 cggtcgcctc ggtggcgacg tcgtacgtgt tgatcctcgc gatcgtgggt ccgatgttga
     2161 cacggttcgt gggggccccg ccgcgggtgc aggcatcatg accgatcagt tcgtgcggtt
     2221 gcgcgatgga cgccaactgg gcttcaccga gtacggcgat ccggcgggct ttcccgtggt
     2281 gaattgccat ggcggtctag cgtgccgact cgacgtcgcg gcggccgacg gtgtcgcccg
     2341 cgacgtctcg atccggctga tttccgcaga tcgccccggg atcgggctgt ccgatccgaa
     2401 accgggccgc acggtgctgg actgggcggc cgacgtctcg gagctggcgg aacacctcgg
     2461 cctgcagcgg ttctcggtga tgggctggtc gatggggggc ccgtacgcgg ccgcggtggg
     2521 ctatgggctg cgcccgcacg tgaaccgggt cgcgctcatc gccagtgccg tcccgctcgg
     2581 cgagcccggc gcgttcgagc agttgccgca gatggaccgc gcctacaccc ggctgtcgca
     2641 gcgctcaccg tggatggccc ggcagtgctt ccgcgcgatg gccttcgtag ccggtgttgc
     2701 cccgagcctc tatggccgcc tcgccgcacg agacctcggg cccgccgacg ggtccgtgat
     2761 acgcaacgag ggtttcggca cgttcgcccg gatgtcacgt gaagcgctgc gccgaccatc
     2821 gggcgtcgtc gaggaatacc gggcgtggat gcggccgtgg gg
//
LOCUS       DASWBF010000243        14223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_417205,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000243 DASWBF010000000
VERSION     DASWBF010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417205"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VF874_08840"
     CDS             complement(<1..416)
                     /locus_tag="VF874_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019748054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9176182.1"
                     /translation="MADSSGLLFNPDTYDPAQFDPETRRQLRAVIDWFEQRGKTKLLR
                     DDLDALWVSDFLDFIKRERIFATFLTPSEYGIGDPNKRWDTSRNAALSEIFGFYGLAY
                     WYAEQVTILGLGPIWQSDNIKAKERAAEQLEAGGVMA"
     gene            complement(540..1349)
                     /locus_tag="VF874_08845"
     CDS             complement(540..1349)
                     /locus_tag="VF874_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9176183.1"
                     /translation="MSTDPALAAKHRAMWASGDYPAVVTDLVARLGPILVEATGIGPA
                     DRVLDVAAGTGNVAIPAAQTGAFVVASDLTPELLAAGQQIAAELGVALEWQEANAEDL
                     PFADNEFDAVLSSLGVMFAPHHQPAADELVRVCKPGGRIGVISWTPEGFIGQMFATMK
                     PYVPAPPPGVQPPPLWGSEDHVRALLGERVEEFSTHRGTLTVDHFPTGAAFRDYFKAN
                     YGPTITAYRSISDDAERVAALDAELAELGDRHLAGSTTMEWEYLLVTARKR"
     gene            1394..1594
                     /locus_tag="VF874_08850"
     CDS             1394..1594
                     /locus_tag="VF874_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176184.1"
                     /translation="MRTPDDPVAVLQRWADAGAVWRVICRHDAGGTVGLYQCDGGEEV
                     DRISSTDPRLLHYLAGRTSSEE"
     gene            complement(1599..2429)
                     /locus_tag="VF874_08855"
     CDS             complement(1599..2429)
                     /locus_tag="VF874_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176185.1"
                     /translation="MGDAGLVPRFAQLPGWDPFRSRERLNALWSAAAVPSVTGGAAIA
                     YRTLFQTLRRLVVGRTLTVRTNGNDLKLTVTEFDFRVEVWGLAVGQVGDVLLAAEDVG
                     WREYRFEQAMAVLSNVHFRPGATSEIVAAPVELSLRLPAEVLEDLVAHASPQFIAHID
                     DDGVVRLRWARRPGWGHLEVDIRIVGTALWLQPRALVVGGRRLGLPARLPIYPVELPD
                     LPNGLLITNVDVRPSCVLLYGLLPEWQMDLPLSRLEDIVSQLTARSGILNLARTAGLM
                     "
     gene            2521..4026
                     /locus_tag="VF874_08860"
     CDS             2521..4026
                     /locus_tag="VF874_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9176186.1"
                     /translation="MRSRALYGLAAAAVAVGVTQLLAAPFGRGADALNAVGSAVVDLT
                     PGPVKEWAIQTFGTSDKLFLSVTMIAVIAVLAAAAGAWETPRRPVGSVLIAAAGAAGC
                     AAILSRPDGGPLDVIPTVVGTLSGILVLRVLIRKGAQPRDDSAPNPSRRQSLTAGGLL
                     IVGVLGGVIGGIVSRRLHSVAGERNAAVLPPAVRPAPPVPPGVQPKDVALPSFVTDNA
                     DFYRIDTALLVPQVSRADWRLRIHGMVDRETTYSFDDLTAFEVVEKAVTLTCVSNPVG
                     GDLIGNAVWRGYRVRDLLARVGIHRDADMVLSTSTDGFTAGTPVEALTDGRESLLAIA
                     MNGEALPVEHGYPARLVVPGLYGYVSATKWVTDLELTRFDRAQAYWTRLGWSARALIK
                     TESRIDVPRGGQRVAVGPVTFGGVAWAQQRGVRAVEVRIDDGPWQTAELSANYSNDTW
                     RLWSYRWTATPGSHTMSVRATDNTGAVQTSEQTPVMPDGATGWHTVVFSAA"
     gene            4039..5571
                     /locus_tag="VF874_08865"
     CDS             4039..5571
                     /locus_tag="VF874_08865"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9176187.1"
                     /translation="MLLADVAAASADVGAKSSRLAKTARIASLLDAAATGGDARLVAV
                     VVSWLSGELVQRQIGVGWAALRTLPPPADEPNLTVAEVDAAFTAIGAVAGAGSQKQRA
                     ALVAQLFCAATAIEQTFLRRLLAGELRQGALAGVMADAVARAAGLSPAQVRRAAMLAG
                     DLPAVAAAALTGGVDALGEFTLRVGRPVGPMLAQTATDVADALERLGSTVVFEAKLDG
                     ARLQIHRDGDNVALYTRSLDDVTARLPEVVDAALALPVSSMIADAEAIALRPDGRPHR
                     FQFTASRFGRAEAKDAQRLSVFFFDVLHLDGDDLLDLPTTERLAVLDTIVPATQRVDR
                     LATADPDEAGSFLDATLAAGHEGVMAKSLTAPYEAGRRGAGWLKVKPVHTLDLVVLAV
                     EWGSGRRTGKLSNIHLGARDPQTGGFVMLGKTFKGMTDAMLEWQTQRFTELADGPTDG
                     YVVKLRPEQVVEIAFDGVQGSTRYPGGMALRFARVLRYRDDKGPAEADTIDTVRELYE
                     RS"
     gene            5673..7985
                     /locus_tag="VF874_08870"
     CDS             5673..7985
                     /locus_tag="VF874_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEX9176188.1"
                     /translation="MAATPLDLTEETEGDVTYVRTDPELPPVVVIDRSPITTRHKIVF
                     GVIALVGAVAWAIIAFVRGETVNAVWFVIAAICTYVVAFRFYARLIEMKIVRPRDEEA
                     TPAELFENATDYMPTDRRVLFGHHFAAIAGAGPLVGPVLAMQMGYLPGIIWILVGAVF
                     AGCVQDYLVLWVSTRRRGRSLGQMVRDEIGPVGGAAAIVAVLVIMVILLAVLALVVVN
                     ALAESPWGVFSIAMTIPIAVLMGLYLRFLRPGRVSEVTLIGVVLLLLAVVAGNWVAET
                     AWGQDWFGLSKVALSWCIIVYGFAASVLPVWFLLAPRDYLSTFMKVGTIALLAVGILI
                     ARPVIEAPAVSAFAHSGTGPVFAGSLFPFLFITIACGALSGFHSLIASGTTPKMLEKQ
                     GQMRLIGYGGMLTESFVAVMALVTAAILNQHLYFVINAPAGKTGIAEGGADLSRYADT
                     AAAYVNTLPIGGDPIQGDEIRRAAADVGETSIVSRTGGAPTLAIGMSEVLHQAFGGPK
                     LQAFWYHFAIMFEALFILTTVDAGTRVARFMLSDALGNLGRPLRKFRNPSWRPGAWLC
                     SLIVVAAWGGMLLMGVTDPLGGIYTLFPLFGIANQLLAAIALTLVTVVVVKKGYLKWV
                     WIPGVPLLWDLTVTLTASWQKIFSADPAVGYWALHNQCQQVLEAGKTRCSTAKNVDEV
                     HDVIRNTFIQGTLSIVFAVLVVVVFATGVIMVLRVIRGGGTPLTEDEPVPSRLFAPSG
                     LIPTAKEREVQRQWDALPKSSAKSVGTVAH"
     gene            7931..8122
                     /locus_tag="VF874_08875"
     CDS             7931..8122
                     /locus_tag="VF874_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEX9176189.1"
                     /translation="MGRATQIVRQIRWYCGTLMGDSHYQRYVEHRQRRHPGERVLSER
                     EYWKMRHAASETNPGARCC"
     gene            complement(8196..8608)
                     /locus_tag="VF874_08880"
                     /pseudo
     CDS             complement(8196..8608)
                     /locus_tag="VF874_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216491.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
     gene            complement(8677..8928)
                     /locus_tag="VF874_08885"
                     /pseudo
     CDS             complement(8677..8928)
                     /locus_tag="VF874_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
     gene            complement(8898..9527)
                     /locus_tag="VF874_08890"
     CDS             complement(8898..9527)
                     /locus_tag="VF874_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176190.1"
                     /translation="MTVDIDPITDASPIDVDIDERIDVADEAEAADEADLIDAADAPA
                     ERSARFGWTRLLIYGVLPGLALILALGAGYLKWQDGSAQLSQTAAAQSVQAATDSTIA
                     ILSYQPDTVDRDLTAAADRLTGDFRDQYTQLITAVVTPGAKQMHIAAIATVPAASLVS
                     ATANHAVVLVFVNQTTIIGNDPPTDTTSSVRVTLEKVGDRWLISQFEPV"
     gene            complement(9569..10183)
                     /locus_tag="VF874_08895"
     CDS             complement(9569..10183)
                     /locus_tag="VF874_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mce protein"
                     /protein_id="HEX9176191.1"
                     /translation="MAGHADSAEQIDDSALTDEKSDVDDRAERPPRKLLSRRAGDIGL
                     LSGTRSAIVVGLVIVAAVAGLSGWLGYRDDQSREDQAQRNLYVQVARQAAVNLTTVNY
                     TEVDADIQRILNSATGAFHDDFQKRSQPFVDVVKQVQSKSEGTVAEAGLVSETGDEAQ
                     VLVAVAVKTSISAAPEQEPRRWRLRITVQKTADGAKVSNVEFVP"
     gene            complement(10150..11649)
                     /locus_tag="VF874_08900"
     CDS             complement(10150..11649)
                     /locus_tag="VF874_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9176192.1"
                     /translation="MLRLTRPVWTQLAILAAITVVAVGVMAFGFVKVPALLGIGRYTV
                     TVDLPASGGLYPTSVVNYRGTEVGSVKSIDVTDDGVGAVLTLDSSIAIPSNVSAAVHS
                     RSAVGEQFLELTPQADAAVDTDSKLRDGDVIPVAKTQIPADIGGLLDATNTALQAIPQ
                     DNLRTVVDEAATAVAGLGPELSRIVDGSTALAIEGGKTIDPLGQLIDQSPPVLNAQVQ
                     TGDSIATWAQRMASITGQLRAEDAAFAELLNIGGPALREGQALFDRVAPSLPVLLANL
                     VSLGEIAVTYHNSIEQLLVLFPQGTAVMSAIALANADTKQDYRGIYLDFNLNLNLPPP
                     CNTGFLPVQQQRSPSDQDYPERPAGDLYCRVPQDSDLNVRGVRNIPCANNPAKRAPTV
                     EICESDEQYVPLNDGYNWKGDPNATLSGQGVPQYPPGTDPRLAPPVGTGTALPNAAGP
                     LPAPSVAFVPYDPATGTYVAPYGQIYTESDLAESTKGKTWQDMLIPPSR"
     gene            complement(11651..12772)
                     /locus_tag="VF874_08905"
     CDS             complement(11651..12772)
                     /locus_tag="VF874_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9176193.1"
                     /translation="MRRGLAAALIVASLSATSACEWRGLNSLSLPGTEGSDDGSYTIE
                     AQLPDVVVIQQNTRVRVADVNVGNVTKIEVQDWHALVTMRINGDVHLPANSTAKVGQT
                     SLLGSMHIELAPPTDEPPEGELKDGSVIRLSRASTYPTTEQTLASVSILLNGGGLGQL
                     QEINQAFATALAGRENDMRSLLTQLDTFISQLNAQTDDIIAATESLNSLAGQVAAKDQ
                     TVDRALTTIPQALAVLSDSRTKLTDAIDALGKFGAIATSTVQQTKESLVNNLRSIAPV
                     LRELANGGPALTRGLDFLSTYPWVKSTLANWFRGDFANITLVIDLTLSRIDSALFTGT
                     RWEGDLTELELQWGRTIGQMPSPYTAGNPLIAPYHFGGY"
     gene            complement(12769..14127)
                     /locus_tag="VF874_08910"
     CDS             complement(12769..14127)
                     /locus_tag="VF874_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9176194.1"
                     /translation="MSSGDDRRAKRLRAVRIATAVSLIVILAVAISVVATPWWDRVSR
                     NTYVADFANTNGLYTGDEVRILGVAVGTVEKIEPQPNSAKVTFSVDRQYPVPADVQAV
                     ILSPSLVTARAMQLVPAYSSGPKLADGATIPQNRTAVPVEWDDLRQQLEKLTDSLQPT
                     TPGGPSAVGEFINSAADNLRGEGDTARDAVIKLSQAVSALGDHSTDIFSTVRNLQLLV
                     SALSSSSDLLAAFNTNLADITTVLSNTPNEVADAARGLDGAVNDLRGFVAENREGLGV
                     TFDRLNSITTALNDSRGDLKHVLHIAPTVFQNFTNIYQPAQSAITGILAPVNFANTVQ
                     FICSAIQAASRQGWEQSSKLCVQYLAPIIKNRQYNFPPLGINPFAGASARPNELTYSE
                     DWLNPHVPPPATPDVQAAANNPAGPLPAEASLPGDLPPGATPTDPSQGLAGLMVPPGG
                     TP"
BASE COUNT         2255 a         4608 c         4831 g         2529 t
ORIGIN      
        1 gccatcacgc cgcccgcctc gagttgctcg gccgcgcgct ccttggcttt gatgttgtcg
       61 ctctgccaga tcggcccgag gccgaggatc gtgacctgct cggcatacca gtacgccagc
      121 ccgtagaagc cgaagatctc gctcagcgcg gcattgcgcg acgtgtccca acgcttgttc
      181 ggatcaccga tgccgtactc cgatggcgtc aggaaggtcg cgaagatccg ctcccgcttg
      241 atgaagtcga gaaagtccga cacccatagc gcgtccagat cgtcccgcag cagcttcgtc
      301 ttgccgcgct gctcgaacca gtcgatcacg gcccgcagtt gacgccgcgt ctcgggatcg
      361 aactgcgccg ggtcataggt gtccgggttg aatagcagtc cgctggagtc ggccatgacg
      421 tatccccttc cgccgagcag acgcaaaagt acccaaaatc cggggctaac ggtgggctaa
      481 cgggcgcttt tgcgttgtcc cgcaagcggg aggtgccccc agctcgcggg gagcgcgact
      541 cagcgctttc gcgcggtgac gagcaggtac tcccactcca tggtcgtcga ccccgccaag
      601 tgccggtcac ccagttcggc cagctccgcg tccagcgcgg cgacgcgctc ggcgtcatcc
      661 gagatgctgc ggtaggccgt gatcgtcggc ccgtaattgg ccttgaagta gtcgcggaac
      721 gcggcgccgg tcgggaaatg gtcgacggtt agcgtgccgc gatgggtgct gaattcctcc
      781 acgcgttccc cgagcagcgc ccgcacatgg tcctcgctgc cccacaacgg cggcggctgc
      841 acacccggcg gcggcgcggg tacgtacggc ttcatcgtcg cgaacatctg cccgatgaag
      901 ccttccggcg tccagctgat cactccgatc cgaccgcccg gtttacagac ccggacgagt
      961 tcgtcggccg cgggctggtg gtgcggtgcg aacatcaccc cgaggctgga gagcaccgcg
     1021 tcaaactcgt tgtcggcgaa cggcaaatcc tcggcgttcg cctcctgcca ctccaacgca
     1081 acgccaagct cggcggcgat ctgctgaccg gctgccaata gctcgggggt cagatcgctg
     1141 gcgacgacga acgcaccggt ttgtgccgcc gggatcgcga cgttgcccgt gcctgccgcc
     1201 acgtcgagca cccggtcggc gggcccgata cccgtcgcct cgaccagtat cgggccaagc
     1261 cgtgcgacca ggtcggtgac gacggccgga tagtcgccgg acgcccacat cgcccggtgc
     1321 ttcgccgcca gcgcagggtc ggtgctcatg tcaccatggt cccccacagg ccaggcgtag
     1381 gagggagaac ggcgtgagaa cgcccgacga cccggtcgcc gtgctgcaac gctgggcgga
     1441 cgccggggcg gtgtggcggg tgatctgccg ccacgacgcc ggggggacgg tcgggctgta
     1501 tcagtgcgac ggcggcgagg aggtcgaccg aatcagctcg acggacccac ggctgctgca
     1561 ctacctggcg ggccgcacca gcagcgagga gtagcccgtc acatcaaccc ggcggtgcgc
     1621 gcgaggttga gtatcccgct gcgggcagtg agctgagaga cgatgtcctc caaccggctc
     1681 agcggcaggt ccatctgcca ctcgggcagc agcccgtaca gcaacacgca actgggccgc
     1741 acatcgacat tggtgatcag cagtccgttc ggcaggtccg ggagctcgac gggatagatc
     1801 ggtaggcgcg ccggaaggcc cagccgccgt ccgccgacca cgagcgcccg cggttgcagc
     1861 cacagggcgg tgcccacgat ccggatgtcc acttccagat gtccccaccc cggtcggcgg
     1921 gcccagcgca gccgcaccac tccgtcgtcg tcgatgtggg cgatgaactg cggcgaggcg
     1981 tgcgcaacga ggtcctcgag tacttccgca ggcagcctca acgagagttc caccggcgcg
     2041 gcgacaatct cggatgtcgc gccggggcgg aagtgaacat tgctcagcac ggccatcgcc
     2101 tgctcgaatc ggtattcccg ccagccgacg tcctcggcgg ccagcaacac gtcaccgacc
     2161 tgtccgacgg ccagccccca gacctcgacg cggaagtcga actcggtgac cgtcaacttc
     2221 aggtcgttgc cgttggtccg caccgtgaga gtgcgaccga cgacgaggcg gcgcagcgtc
     2281 tggaacagcg tccggtacgc gatcgcggca ccgccggtga cgctgggcac cgcggccgcc
     2341 gaccacagtg cgttcagccg ctcgcgagac cggaacgggt cccagccggg cagctgtgcg
     2401 aagcgcggca ccagtccagc atcccccatc cgtcaatcca tgatgcgcgg gcgacgttcg
     2461 aatgcgaggt gtcactgctg tgtaaggcct ttccgcgcag gtcgcgggta ggttgacggc
     2521 atgcggagcc gggctctcta cggcctcgcg gcggccgctg tggccgtcgg ggtcacccag
     2581 ctgctcgccg cgccgttcgg ccgcggcgcc gatgcgctca acgcggtcgg ctcggccgtc
     2641 gtcgacctca cgccggggcc ggtcaaagag tgggccatcc agacgttcgg cacctcggac
     2701 aagctgtttc tgtcggtcac gatgatcgcg gtgatcgcgg tgttggcggc ggccgccgga
     2761 gcgtgggaga cgccgcgccg gccggtgggc agtgtgttga tcgccgcggc gggcgccgcc
     2821 gggtgcgcgg cgattctgtc ccggcccgac ggcggaccgc tcgacgtgat ccccaccgtt
     2881 gtcggcaccc tcagcggaat tctcgtgctg cgggtcctca ttaggaaagg tgctcagcca
     2941 cgggacgatt cggcgcccaa tccgtcccgc cggcagtcac tcaccgccgg cgggctgctg
     3001 atcgtcggcg tgctgggcgg cgtgatcggc ggcatcgtca gccgtcggct gcactccgtg
     3061 gccggtgagc gcaatgccgc ggtactccca ccggccgtgc ggccggcgcc gccggtgccg
     3121 cccggtgttc agccgaaaga cgttgcgctg ccgagctttg tcacggataa cgccgatttc
     3181 taccggatcg acacggcgtt gctggtcccc caggtcagcc gcgctgactg gcggttgcga
     3241 atccacggca tggtggaccg cgaaacgacg tacagcttcg acgatctcac cgcgttcgaa
     3301 gtcgtcgaga aggcggtgac gctgacctgc gtctccaatc ccgtcggcgg cgacttgatc
     3361 ggcaacgcgg tctggcgggg ctaccgcgtg cgagatctcc ttgcgcgggt gggaattcac
     3421 cgcgacgccg acatggtgct gtcgacgtcg acggacgggt tcaccgcggg aacgccggtc
     3481 gaggcgttga ccgacggccg cgagtcgctg ttggcgatcg cgatgaacgg cgaggcactg
     3541 cccgtggaac acggctatcc ggcgcggctg gtggtgcccg gcctgtacgg atatgtgtcc
     3601 gcgacgaagt gggtcaccga tctggagctg actcggttcg accgcgctca ggcgtactgg
     3661 acgcggctgg gctggtcggc acgcgcactg atcaagaccg aatcgcgcat agacgtaccg
     3721 cgcggcggcc aacgcgtggc cgtcggaccg gtcacgttcg gcggtgtggc atgggcacaa
     3781 cagcgcggcg tgcgcgccgt cgaggtccgc atcgacgacg ggccttggca gacagccgaa
     3841 ctcagcgcca actactccaa cgacacctgg cggctgtgga gctaccgctg gacggccacc
     3901 cctgggtcgc acaccatgag cgtccgggcc accgacaata ccggcgccgt ccagacgtcc
     3961 gagcagacgc cggtgatgcc cgacggcgcc actggttggc acacagtcgt gttcagcgcg
     4021 gcatgacaag ctaggcgcat gctgctcgcc gacgttgccg ccgcctcggc ggacgttggt
     4081 gcgaagtcgt cgcggctggc caagaccgcc cgcatcgctt cgttgcttga cgctgcggca
     4141 accggcggcg acgcgcgctt ggttgcggtc gtggtttcgt ggctgtcggg cgagcttgtc
     4201 cagcggcaga tcggcgtcgg gtgggccgcg ttgcgcaccc tgccgccgcc ggctgacgag
     4261 ccgaacctca ccgtggccga ggtcgacgcg gcgttcaccg cgatcggtgc agtggccggc
     4321 gcaggctcac agaagcagcg ggctgcgctt gtcgcacagc tgttctgcgc ggcgacggcg
     4381 atcgagcaga ccttcctgcg ccgcctgctg gctggagaac tgcgccaggg cgccctggcc
     4441 ggtgtgatgg ccgacgccgt cgcacgggcg gccggcctct cccccgctca ggtgcggcgc
     4501 gccgcgatgc tcgccggcga cctgccggcc gtcgcggccg cggccttgac gggtggcgtc
     4561 gacgcactcg gcgaattcac gctgcgggtt gggcgaccgg ttggcccgat gctcgcacag
     4621 accgcgaccg acgtcgctga tgcactcgaa aggctgggca gcacagttgt tttcgaagcg
     4681 aagctggacg gtgcgcggct gcagatccac cgcgacggcg acaacgtcgc cctgtacacc
     4741 cgcagcctcg acgacgtcac ggcccggctg cccgaggtcg tcgacgccgc gttggcgctt
     4801 cctgtctcgt cgatgatcgc cgacgctgag gcgatcgcgc tgcggcccga cggccgaccg
     4861 caccgcttcc aattcaccgc gtcccggttc gggcgcgccg aagctaaaga cgcgcaacgg
     4921 ctttcggtgt tcttcttcga cgtcctgcac ctcgacggcg atgacctctt ggaccttccg
     4981 acgacggagc gcctcgccgt gctcgacacg atcgtgcctg ccacgcagcg cgtcgaccgg
     5041 ctggccaccg cagaccccga cgaagcaggc tcgttccttg atgcgacgct ggcggccggt
     5101 cacgaggggg tgatggcgaa gtcgctgacc gcgccgtacg aggccggccg gcgcggggcc
     5161 ggatggctga aggtcaaacc ggtgcacacc ctcgacctgg tggtgttggc cgtggaatgg
     5221 ggctcggggc ggcgcaccgg caagctgtcc aacatccacc tcggcgcccg cgacccgcag
     5281 acgggtggat tcgtcatgct gggcaagaca ttcaaaggca tgacggacgc gatgctcgag
     5341 tggcagacgc agcggttcac ggaattggcc gacgggccaa cggacgggta cgtggtcaag
     5401 ctgcgccccg agcaggtggt cgagatcgcg ttcgacgggg tgcagggttc gacccgctac
     5461 ccgggcggca tggcgctgcg attcgcgcgg gtgctgcgct accgcgacga caagggtccc
     5521 gccgaggccg acaccatcga caccgtgcgg gaactgtacg agcggagcta gcgaagaccc
     5581 acctcgccga gactgcgact accgcgaaga agtgcgagta cggaccacgt ggaattcaat
     5641 ttcggcggac gaatgcatga caagatctcg gcatggccgc cacaccgctg gatctcaccg
     5701 aagaaaccga gggcgacgtc acctacgtcc ggaccgaccc cgaactgccg ccggtcgtgg
     5761 tcatcgaccg ctccccgatc accacccgcc acaagatcgt gttcggcgtc atcgcactgg
     5821 tgggcgccgt ggcctgggcg atcatcgcgt tcgtccgcgg cgagaccgtc aacgccgtct
     5881 ggttcgtgat cgcggcgatc tgcacgtacg tcgtcgcatt ccgcttctac gcgcgcctga
     5941 tcgagatgaa gatcgtccgg ccccgcgacg aagaggccac cccggccgaa ctgttcgaaa
     6001 acgccaccga ctacatgccc accgaccggc gggtgctgtt cggtcaccac ttcgcggcga
     6061 tcgccggcgc cggcccgctg gtcggacccg tgctggcaat gcagatggga tacctgcccg
     6121 gcatcatctg gatcctcgtc ggcgcggtgt tcgcgggctg cgtgcaggac tacctggtgc
     6181 tctgggtgtc cacccgccgc cgggggcggt ccctcggaca gatggtgcgc gacgagatcg
     6241 ggcccgtcgg cggggccgcc gcgatcgtcg cggtactcgt catcatggtg atcctgctgg
     6301 cggtgctggc gctcgtggtc gtcaacgcgc tggccgagag cccctggggc gtgttctcga
     6361 tcgcgatgac gatcccgatc gcggtcttga tgggcttgta tctgcgcttc ttacggcccg
     6421 gccgggtctc ggaggtcacg ctgatcggtg tcgtcctgct gctgttggcg gtcgtcgccg
     6481 gtaactgggt ggccgaaaca gcctggggcc aagactggtt cggcctgtcc aaggtcgcgc
     6541 tgtcgtggtg catcatcgtc tacggcttcg cggcgtccgt gctcccggtg tggttcttgc
     6601 tcgctcctcg cgattacctc tccacgttca tgaaggtcgg cacgatcgcg cttctggccg
     6661 tcggcatcct gatcgcccgg ccggtcatcg aagcgcccgc ggtgtccgcg ttcgcgcaca
     6721 gcggcacggg gccggtgttc gccggttcgc tgttcccgtt cctgttcatc acgatcgcgt
     6781 gcggcgcact gtcgggattt cactcgctga tcgcctccgg caccacgccg aaaatgctgg
     6841 agaagcaagg ccagatgcgg ctgatcggct acggcggcat gctcaccgag tcgttcgtcg
     6901 ccgtcatggc gctggtgacc gcggcgatcc tgaaccaaca cctctatttc gtgatcaacg
     6961 ccccggccgg caagaccggc atcgcagagg gcggcgccga cctctcccgc tacgcggaca
     7021 ccgcggcggc gtacgttaac acgcttccga tcggcggcga cccgattcaa ggcgacgaga
     7081 tcagacgcgc cgccgccgac gtcggagaga cgtcaatcgt ctcgcgcacc ggtggcgcac
     7141 cgacgttggc aatcggcatg tcggaggtgc tgcatcaagc attcggcggt cccaagctcc
     7201 aggcgttctg gtaccacttc gcgatcatgt tcgaggcgct gttcatcctg accacggtcg
     7261 acgccggcac ccgcgtcgcg cggttcatgc tttccgacgc gctcggcaac ctcggccggc
     7321 cactgcgcaa gttccgcaat cccagctggc ggccgggtgc atggctgtgc agcctgatcg
     7381 ttgtcgcggc ctggggcggc atgcttctga tgggggtcac cgacccgctc ggcggcatct
     7441 acacgctctt cccgctgttc ggtatcgcca accagttgct tgcggcgatc gcgctgacac
     7501 tggtgacggt ggtcgtggtc aagaagggtt atttgaaatg ggtttggata ccgggtgttc
     7561 cgctgctgtg ggacctcacg gtgacgttga ccgcgtcgtg gcagaagatc ttctccgctg
     7621 atcccgccgt cgggtactgg gcgctgcaca accagtgtca gcaggtcctc gaggccggaa
     7681 agaccaggtg cagcacggcg aaaaacgtcg atgaagtgca cgatgtcatt cgcaacacgt
     7741 tcatccaggg cacgctgtcg atcgtgttcg cggtgctcgt cgtcgtcgtg ttcgccaccg
     7801 gtgtgatcat ggtgctcagg gtgattcgtg gcggcggcac gccgctgacc gaagacgagc
     7861 cggtgccgtc gcgactcttc gcgccatcag ggctcatccc gacggccaaa gaacgggagg
     7921 tgcaacggca atgggacgcg ctacccaaat cgtccgccaa atccgttggt actgtggcac
     7981 attgatgggc gacagccact accagagata cgtcgagcac cggcagcgac gccaccccgg
     8041 cgagcgcgtc ctcagcgagc gcgagtactg gaagatgcgc cacgccgcca gcgaaaccaa
     8101 cccgggggcg cgctgctgct gacgtcagag caccggcgag ccgtcgcagg attcaggagt
     8161 cggcgcagag gactgggcga agtaaccggt cagcccggct ggccgtagac agcgaccacg
     8221 acggtgcggt cgaggctatt cgccgaccac accccgatgg cggtgttggc gcaatcagac
     8281 atgatcgcga agtcggctgg gtcgtagtac cactggttga ggatctcgat gccgctgatc
     8341 gccagggccg ggttgatggc cacggtttgg gccactttgc cgcggaatcc ggcggcctcg
     8401 gcgcgggatt gcggggtgga cccgtcagag ccgatgtcac cgtcgaggcc gcggttgttg
     8461 agcacgtcgt tggtgtgcca ctccgctgct agctgcagtt ggagattgat cttgacgtcg
     8521 ttggtgcacc cggcctgttg ctgaatggtg tagacattgg ccactacgcc gtcgttgagt
     8581 cgcttgttgt ggcgtaggca gcgggcattg ccagggcgcc agcggcgaac agcgtcgccg
     8641 caataggcaa cgccgacgct cgacgccatg gatgcctcac ggcaccgccg gtcggtagtt
     8701 cgcggccgag tcgcgcagtt gccaaatcat cgcggggcaa agttcgttga ccgcttggtt
     8761 gatcagatat ccggcctgat actcatctgt ggtgccgaag tcagctttga cgtcgtccac
     8821 gagctgagcg taggtgcggg ctgcggcgac tttgtcgcag atgccgtgcc cgtaggccag
     8881 cgcggcgtcg gcgtaattca caccggttcg aattgcgaga taagccatcg atcaccgacc
     8941 ttttcgaggg ttacgcgcac gctggaggtg gtgtcggtcg gcggatcgtt gccgatgatg
     9001 gtggtctgat tgacgaaaac caacaccacc gcgtggtttg ccgtcgccga taccaacgac
     9061 gccgccggca ccgtggcgat cgccgcgatg tgcatctgct tggcaccggg agtaacgact
     9121 gccgtgatga gctgtgtgta ttggtcacgg aagtcgcccg tgagccggtc agccgccgcg
     9181 gtcaggtctc ggtccacggt gtctggctgg tacgacagga tcgcgatcgt gctgtcggtg
     9241 gcggcttgta cagactgcgc ggctgccgtc tgcgatagtt gcgccgatcc gtcctgccat
     9301 ttcagataac cggcccccag ggcaaggatc agggccaaac cgggtagcac accgtagatc
     9361 agaaggcgag tccagccgaa gcgggcggac cgttcggccg gagcgtcagc cgcgtcgatg
     9421 aggtcagctt cgtcggccgc ttcagcttcg tcggccacat cgattcgctc gtcgatgtcg
     9481 acatcgatcg gcgatgcgtc ggtgatgggg tcaatgtcga ccgtcatttc ggagttctcc
     9541 ccctgtgtgg cgagagctgt tcggctgctc acggcacgaa ctcgacgttc gataccttgg
     9601 cgccatcggc cgttttctga acggttatcc gcaaccgcca tctgcgcggc tcttgttccg
     9661 gcgcagcgga aatcgacgtt ttcaccgcca ccgcgaccag cacctgcgcc tcatcaccgg
     9721 tttcggatac cagaccggct tcggcgaccg tgccctccga cttcgactgc acctgcttga
     9781 cgacatcgac gaacggctgg gatctcttct ggaaatcatc gtgaaaggcg ccggtcgccg
     9841 aattcaggat ccgctgaatg tcggcgtcga cctcggtgta gttgaccgtg gtgaggttga
     9901 ccgccgcctg acgagcgacc tgcacgtaca ggttccgctg tgcttggtcc tcgcggctct
     9961 ggtcatcgcg gtatcccagc caaccactga gaccggccac ggccgcgacg atgacaagac
    10021 ccaccacgat cgccgagcgt gtgcccgaca acagcccgat gtcgccggcc ctgcggctca
    10081 ggagctttcg cggcggcctt tcagcgcggt cgtccacgtc gcttttctca tccgttagtg
    10141 ccgaatcgtc tatctgctcg gcggaatcag catgtcctgc catgtcttgc ctttcgtgct
    10201 ttcggccaag tcggactccg tgtagatctg gccatatggg gccacgtaag tgccggtggc
    10261 cgggtcgtag ggaacgaagg cgaccgacgg cgcgggcagc gggcccgccg cattgggtag
    10321 cgctgtgcca gtgcctaccg gcggcgccag ccgtggatcg gtgcccggcg gatattgcgg
    10381 caccccctga ccggagagcg tcgcgttggg gtcacctttc cagttgtagc catcgttgag
    10441 tgggacgtac tgctcgtcgc tttcgcatat ctcgacagtc ggggcgcgtt tggcggggtt
    10501 gttcgcgcat ggaatgttgc gcacgccacg gacattgagg tccgagtcct gcggcacccg
    10561 gcagtacagg tcaccggcgg gacgctcggg atagtcctga tcggagggcg accgctgctg
    10621 ctggaccggc aggaatccgg tgttgcaggg cggcggcagg ttgaggttga ggttgaagtc
    10681 gaggtagata ccccggtaat cctgtttggt gtcggcgttc gccagggcta tcgccgacat
    10741 gaccgcggtc ccttgcggga agagaaccag cagctgctcg atgctgttgt ggtaggtcac
    10801 cgcgatttcg ccgagactca cgagattggc cagcagcacc ggcaaggagg gggcgacccg
    10861 gtcgaacagc gcttggcctt cacgcagggc cggaccaccg atgttgagca gttccgcgaa
    10921 ggccgcgtcc tccgccctga gctgaccggt gatcgaggcc atccgctgcg cccaggttgc
    10981 gatcgaatcc cccgtctgca cttgggcatt aagtacgggc ggagactggt cgatcaactg
    11041 tccgagcgga tcgatggtct tgccgccctc gatcgccagc gccgtcgatc cgtcgacgat
    11101 gcgtgacagt tcgggtccca gcccggcgac ggctgtggct gcttcgtcga cgacggtgcg
    11161 cagattgtct tgcgggatcg cttgcagcgc tgtgttggtg gcgtcgagca ggcctccgat
    11221 gtcggcggga atctgcgtct tggcgactgg gatcacatcg ccgtcccgca gcttcgaatc
    11281 ggtgtcgacg gccgcgtctg cttgcggggt caattcgagg aactgttcac cgacggccga
    11341 gcggctgtgt accgcggccg aaacgttgga cggaatcgca atggacgagt ccagtgtcag
    11401 aacggcacca acaccgtcgt ctgtgacgtc gatcgatttg acgctgccga cctcggtacc
    11461 gcggtagttc accactgacg tcgggtacaa cccgccggac gcgggaaggt cgaccgtcac
    11521 cgtgtatcgc cctattccga gcaacgccgg tactttcaca aagccgaacg ccatcacgcc
    11581 gacggcgacg acggtgatcg ccgcgaggat cgccaactgt gtccacaccg gacgggtcaa
    11641 gcgcagcatg ctaatacccc ccgaagtgat agggggcgat cagcgggttg cccgcggtgt
    11701 atgggctcgg catctggccg atcgtgcggc cccactgtag ttcgagctcg gtgaggtccc
    11761 cttcccacct cgtcccggtg aacaaggcgc tgtcgatccg actcagggtc agatcgatga
    11821 cgagagtgat gttggcgaag tcacctcgga accagttggc aagggtgctc ttcacccacg
    11881 ggtatgtcga caggaagtcc agccccctgg tgagggcggg cccgccgttg gccagttccc
    11941 gcagcaccgg cgcgatgctg cgcaggttgt tcacaaggga ttccttggtc tgctgaaccg
    12001 tcgaggtggc gatggcgcca aatttgccta gtgcgtcgat cgcgtccgtc aatttggtgc
    12061 gggaatccga cagcacggcc agcgcctgcg ggatcgtggt gagcgccctg tcgacggtct
    12121 ggtccttcgc cgcaacctgc ccggccaacg aattgagact ctcggtggcg gcgatgatgt
    12181 cgtcggtctg ggcgttgagc tgtgaaatga aggtatccag ctgggtcagc aggctgcgca
    12241 tgtcgttctc acggcccgcc aaagccgttg cgaacgcctg attgatctcc tgcagctggc
    12301 ccagcccgcc gccgttgagc agaatcgaca ccgacgccaa ggtctgctcg gtggtcggat
    12361 atgtactggc cctggataac cgaatcaccg aaccgtcctt gagttcgccc tctggcggct
    12421 cgtcggtcgg cggtgcgagt tcgatgtgca tggagcccag aaggctggtc tgtccgactt
    12481 tggcggtgct gttcgctggt aggtgtacgt cgccgttgat gcgcatggtt accagggcat
    12541 gccagtcctg tacctcgatc ttggtgacgt tgccgacgtt gacgtcggca acgcgcaccc
    12601 gggtgttctg ttgaatgacg accacatcgg gcaactgcgc ctcgatggtg tacgaaccgt
    12661 cgtcgctgcc ctcggttccc ggcaggctca gcgagttgag cccccgccac tcacacgccg
    12721 aggtcgcgga caaactcgcg acgatgagcg ccgcggccag cccgcgcctc acggcgtgcc
    12781 tccgggtgga accatcaggc cggccagtcc ctggctgggg tcggtcggtg tcgcccccgg
    12841 tggcagatca ccgggcagcg acgcctctgc cggcagtggc ccggccgggt tgttcgcggc
    12901 cgcttggaca tccggcgtcg ccggcggcgg cacatgcgga ttcagccagt cttcgctata
    12961 ggtgagctcg ttgggccgcg ccgaggctcc ggcgaacggg ttgataccga gcggcggaaa
    13021 gttgtactga cggttcttga tgatcggcgc cagatactgg acacacaact tcgacgattg
    13081 ctcccaaccc tgacgcgacg ctgcttggat tgcgctgcaa atgaattgga cggtgttggc
    13141 gaagttgacc ggggccagaa tgccggtgat cgcgctctgc gccggctggt agatgttcgt
    13201 gaaattctgg aacaccgtcg gcgcgatatg caggacgtgc ttgaggtcac cgcggctgtc
    13261 gttcaaagcc gtggtgatcg aattgagccg gtcgaaggtt acgccgagcc cctctcggtt
    13321 ctcggcgacg aagccgcgca ggtcgttcac cgcgccgtca aggccccgcg ccgcatcggc
    13381 cacctcgttg ggcgtattgg acaacacagt ggtgatgtcg gccaggttcg tgttgaacgc
    13441 ggcgagcaga tcactgctgg aggacagggc ggataccagc aactgcaggt tgcgcaccgt
    13501 gctgaagatg tctgtgctgt ggtcgccgag tgccgaaact gcctgcgaga gtttgatgac
    13561 tgcgtcgcgt gcggtgtcac cctcgccgcg caggttgtcc gccgcgctgt tgatgaattc
    13621 gcccaccgcg ctgggtccgc cgggggtcgt gggctgcaag gagtcggtga gcttctccag
    13681 ctgctggcgc aggtcgtccc actcgacggg aaccgcggtg cggttctgcg gaatagtggc
    13741 gccgtcagcc agtttcgggc cgctggagta cgccgggacg agctgcatcg cgcgtgcggt
    13801 gaccagtgac ggcgacagga tcaccgcctg gacatcggcg ggcacgggat actgcctgtc
    13861 aaccgaaaag gtgaccttcg cggagtttgg ctgcggctcg atcttctcca cggttccgac
    13921 ggccacgccc aggatgcgga cctcgtcgcc ggtatagaga ccgttggtat tggcgaagtc
    13981 ggcgacgtag gtattcctgc tgacgcggtc ccaccatggt gtggcaacca cgcttatcgc
    14041 aacggcaaga atgacgatga ggctgaccgc ggtcgcgatc cgcactgcgc gcaatcgctt
    14101 ggcgcggcgg tcgtctccgg aagacacgac agacacggtc actgccctcc ggccgggggc
    14161 gccggaaccg gcggcgggat ggacggcggc gggagctcgc cgggcgccgg cccctgggcc
    14221 ggc
//
LOCUS       DASWBF010000244         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_84272, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000244 DASWBF010000000
VERSION     DASWBF010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84272"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..626
                     /locus_tag="VF874_08915"
     CDS             27..626
                     /locus_tag="VF874_08915"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9176195.1"
                     /translation="MAEPWGDCTYRVVPKRIVAWDYGSLREVSTPPGTSTPRQALFSV
                     TDEKDDVHPLHSPKLEAAVGGSRVMILASRSQKGTPFAVPLWFVSHHGCIYATTSASS
                     WTVRNVAASPQVALLLGGEGRDNADGLLVRGRGRAVRGMPPPAVLARIAWLYDLQPRF
                     AAVELSHIRLWGRRMRYYGESQAAHIVITPQTATECRVP"
     gene            complement(1130..1270)
                     /locus_tag="VF874_08920"
                     /pseudo
     CDS             complement(1130..1270)
                     /locus_tag="VF874_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein Lsr2"
     gene            complement(1274..1579)
                     /locus_tag="VF874_08925"
                     /pseudo
     CDS             complement(1274..1579)
                     /locus_tag="VF874_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
     gene            1888..2484
                     /locus_tag="VF874_08930"
     CDS             1888..2484
                     /locus_tag="VF874_08930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9176196.1"
                     /translation="MSTAQELSVTAALDQGRAIATLTTAFSNDPIARWVLRDADTYLN
                     FWPRLVQVFAGSAFDHGTADSVAKHGGVALWLGPGVGPDEEAMGALVGDAVAPGDQDD
                     VFGFMAQMDEFHPTYPHWYLPLIGVDLPHQGRGYGSVLLVHALRRCDDDQLPAYLEAT
                     NPRNKRLYERHGFEELGVIQIGGSPPMWPMLHKTGLSR"
     gene            complement(2647..3009)
                     /locus_tag="VF874_08935"
     CDS             complement(2647..3009)
                     /locus_tag="VF874_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176197.1"
                     /translation="MAAGYKQLLAVERGWRDCKSSLGLRPVYHHREDRIRAHVQLCWL
                     AVLLIRVAETRVGDTWRNLRHELDRMHLVTLATADGRVAQRSATTSDQKTILAALDLP
                     EPPKYLDFTPGIDAAVAD"
BASE COUNT          545 a         1047 c         1177 g          594 t
ORIGIN      
        1 gattgcgctc gctgaccgtc agagggatgg ccgaaccctg gggcgattgc acttatcggg
       61 tagtgcccaa gcgaatcgtg gcctgggact atggctcact ccgtgaggta tcgacaccgc
      121 caggaacctc aactccacga caggcattgt tttcggttac cgacgaaaag gacgacgttc
      181 acccgttgca ctcgccgaaa cttgaagcgg cggttggcgg gtcgcgagtc atgattttgg
      241 ctagccgttc acagaaggga acaccattcg cggtaccgct gtggttcgtt tctcaccacg
      301 ggtgcatcta tgcgacgacc tcggcgtcgt cctggacggt acgtaatgtg gccgcttccc
      361 cgcaggtggc cctgctgctc ggcggcgagg gtcgggacaa cgccgacggg ctcctggtgc
      421 gcggacgcgg ccgagcggtc cgaggcatgc cgccgccagc ggtgctcgct cggatagctt
      481 ggctctacga cctgcaacca cggttcgcgg cggtagaact gagtcacata cggctatggg
      541 ggcggcgaat gcggtactac ggcgagtcgc aggcagcaca catcgtgatc acgccccaga
      601 cagcgaccga atgtcgggtg ccataggcac agcggcgaca ccgtcggagt ggggatctcg
      661 agctgagatc cgtgatcttt caatccgcgg acccgccact gctgtgccgg gtcggctacc
      721 acgtcgcggc aacgaaccgg agtgagtgac gtaaaaccgc ttggtatcag ccagttttcg
      781 gcgtaccggg cgccgctgac ggcgacgacg agctcagcgg tcggcgagca tgacaatcaa
      841 cgattccaga aggcccgcgt ccttcgggtc gaccggcccg tcggcttcgg cggcggtgat
      901 aagctccgcc gacaacccgg tggcgttggc gacctcctcg actgtcagct cggcgcgccg
      961 ggcgggcggc gtaaaggcgc tgccccagtg tggcgttcgg ggaccgcgcc gtacgcagca
     1021 tcaactcgtc gtagcgtttg cgcaccgtgc tgagcgcgat ggcgatctcc ggtgcaccgc
     1081 gggcgctgcg cgcggcgcgg ccggccaccc cctcgagtcg gcggaggtcc gcttgcttgc
     1141 ggtcgattga tgcgccgccc gtgccgacac cgcgtcgtcg gcgagatcct ccggttcgcc
     1201 gacctgcatt cacccacggc tgcagatgct cgcgcagttc cttggcgttc ttggcggaga
     1261 ggtcgatttc gatgtcggag aagccaactc gttccagcgc atcgagcagc cggggcatgt
     1321 cgcgcggacc gtggtagccc ttgacgagtc cgggaactcc attcatgtcg gtgcccacgc
     1381 attggtggtc gactccgacg aggttggcga tatgttctgc gtggcgggcg agttcgtcga
     1441 gggtgctgac cccgcgtccg ggttggttgt agggccgcag gccgatcagc ccgccggtgg
     1501 cggcgatggt ttgaatgtcg tggtcggcaa ggtagcgcgg gaactggttc agcgccgccg
     1561 cgccggtgtg tgagctcatg actggtcgcg ggtgtgttca cacaccccgg cgacggtggc
     1621 gctgtcggca tggggttacg acgctccgtg atctgacccc aacggggaca actgaatact
     1681 gaggcagggg caccggggct aaccaccccg gggctttctg ctgcccacat gagaggacag
     1741 aggggaggca gcagccgtaa tgaccacaga gcccaccgac gacagagccg tgcgaagggc
     1801 gggcacaatg tcgttgacgg cttgtcatcg tgtgcgagca aatgcaccat gaggtgggcg
     1861 cctttgccgc cttccggagg agtcctcatg tccaccgccc aagagttatc ggtcaccgcc
     1921 gcactcgacc aaggccgcgc aatagccaca ttgaccacgg cattcagcaa cgatcccatc
     1981 gcccgctggg ttctgcggga cgccgacacc tacctgaatt tttggccccg cctagtgcag
     2041 gtgttcgcag gcagtgcgtt cgaccatgga accgcggact cggttgccaa acatggcggc
     2101 gtggcgttgt ggcttgggcc gggcgttggg cccgacgagg aggcgatggg cgccctggtc
     2161 ggcgacgcgg tggccccggg cgaccaggat gacgtcttcg ggttcatggc tcagatggat
     2221 gagtttcacc caacctatcc acactggtac ttaccgttga tcggcgtcga cctgccgcac
     2281 cagggtcgag gctacggatc ggtgttgctt gtccacgcac tgcgcaggtg tgacgacgac
     2341 caactgcctg cctatctcga ggcgaccaac ccgcgaaaca agcgcctcta cgaacggcat
     2401 ggattcgagg agctgggagt cattcagatc ggtgggtcgc cgccgatgtg gccaatgctg
     2461 cataaaaccg gcctgagtcg ctgaagtaca gctgatggcc cgaccgccga gttggtcggc
     2521 gacgctgacc tgacttccgc agatggtggg cacacggatc gccgaatttc tgcgttgacc
     2581 agcgcaaacg tgggatgagc cgatgggtcg tgttactaca cgacgccggt gatccgggcg
     2641 ggttcgtcag tcggcgacgg cggcgtcgat gccgggtgtg aagtcgaggt atttgggggg
     2701 ttcgggcagg tcgagcgcgg cgaggatggt cttctggtcg ctggtggtgg ccgagcgttg
     2761 ggcgacgcgg ccgtcggcgg tggcgagggt gaccaggtgc atgcggtcca gttcgtgacg
     2821 caggttgcgc caggtgtcac cgacgcgagt ttccgcgacg cggatgagaa gcaccgccag
     2881 ccaacacaat tggacgtgag cgcggatgcg gtcctcgcgg tggtggtaga ccgggcgcag
     2941 cccgagcgag ctcttgcagt cgcgccagcc gcgttccacg gcgagcagct gcttgtagcc
     3001 ggcggccaag tcctcggcgg tcagcgtcgg gtcggaggtg cgcagcagcc acttgccgtc
     3061 gtagtgggct tcccattgcg gcggcggtgc ggtcgatgcg cagcagcccg gccttggtgc
     3121 gccgcaccag ccttcgcagc ccgggcttgc ccttcaggga gccgaccagc tcgtcgcgcc
     3181 aaggcactag cggccgatgc ggtggccgcc ctgctgaccg ccctcagcgc cgacccgtcg
     3241 ccgcggcggc gcaccgactg gatgcagcgc gaccgcgccc tgatcctgac cgcgctgctg
     3301 gccgggttgc gcgccgatga actggtgcgc gccaacgtcg gtgaccttcg ccgcaccgac
     3361 gac
//
LOCUS       DASWBF010000245         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_488052,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000245 DASWBF010000000
VERSION     DASWBF010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488052"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1563
                     /gene="nirB"
                     /locus_tag="VF874_08940"
     CDS             <1..>1563
                     /gene="nirB"
                     /locus_tag="VF874_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEX9176198.1"
                     /translation="ATGSYPFVPPIPGMDLDRCFVYRTLDDLDAIRAAVTPGTDAIVV
                     GGGLLGLEAANALRLMGMAPHVVEFAPRLMPAQVDDGGGAVLAALVTDLGLTVHTGKS
                     TTAIETHDDSVRATLSDGTTIDAGLLVFSAGIRPRDELARVCGLDVAERGGVLTDIAC
                     RTSDPYVYAIGEVAAIEGRCYGLVAPGNASAEVVADRLLGGTAEFPGADMSTKLKLLG
                     VDVASFGDAHATTDGALEVVLNDPTKGTYAKLVVSDDASTLLGGILIGDATAYGTLRP
                     LVGRPLPADPASLIAPHGAEVGVGALPDEAQICSCNAVSKGAICSAISEGACDVPAIK
                     GNTAAGTSCGSCIPMLKRLLQAQGVEQSKALCEHFSQSRAELFQIVAATDIRTFSSLI
                     AKYGTGTGCDICKPAVASILASTSSDHVLDGETAALQDTNDHFLANLQRNGTYSVVPR
                     LPGGEITPEKLIAIGEVARDFGLYTKITGGQRIDMFGARVEQLPLIWKRLIDAGMESG
                     HAYGKALRTVKS"
BASE COUNT          259 a          530 c          517 g          257 t
ORIGIN      
        1 tcgccactgg ctcgtatccg tttgtaccgc cgatccccgg catggacctc gaccgctgct
       61 tcgtctaccg cacgctcgac gacctcgacg cgatccgcgc tgcggtaacc cctggcaccg
      121 acgcgatcgt ggttggcggt ggactgctgg ggctggaggc cgcgaatgcg ttgcggctga
      181 tgggaatggc cccgcatgtc gtcgaattcg cgccccggct gatgccggca caggtcgacg
      241 acggcggcgg cgcggtgctg gcggctttgg tgaccgatct cggtctcacg gtgcacaccg
      301 gcaagtccac caccgcgatc gagacacatg acgacagtgt gcgggccacg ctctccgacg
      361 gcaccacgat cgatgccgga ttgctggtgt tctccgcggg gatccggccc cgtgacgagc
      421 tggcccgcgt ctgcggactc gacgtcgccg aacgcggcgg cgtgctgacg gatatcgcct
      481 gccgcacaag cgatccgtac gtttacgcga tcggggaagt cgcagcgatc gagggccggt
      541 gctacgggct cgtcgcgccg ggcaacgcga gcgccgaggt ggtggccgac cgactgctcg
      601 gcggcaccgc cgagtttccc ggcgcggaca tgtcgaccaa gctcaagctg ctgggcgtcg
      661 acgtggccag cttcggcgac gcgcacgcca cgaccgacgg cgcgctggag gtggtgctca
      721 acgacccgac gaagggcacc tacgccaaac tcgttgtgtc cgatgatgcg tcgacattgc
      781 tgggcggcat ccttatcggc gacgcgaccg cctatggcac gctgcggccg cttgtcggcc
      841 gcccgctacc ggctgatccg gcttcgctga tcgctcccca cggtgcggag gtcggtgtcg
      901 gcgcgctgcc cgacgaagcg cagatctgct cgtgcaacgc ggtcagcaag ggtgcgatct
      961 gctcggcgat ctcagaaggc gcgtgcgacg tgcccgcgat caagggcaac actgctgccg
     1021 gaacatcctg tggcagttgc attccgatgc tcaagcggct gctgcaggct cagggcgtcg
     1081 agcaatccaa ggcgctgtgc gaacacttca gccagtcacg ggccgagttg ttccagatcg
     1141 tcgccgccac cgacatccgc accttctcca gcctgatcgc caagtacggc accggaaccg
     1201 gttgcgacat ctgcaaaccg gccgtcgcgt cgatcctggc gtcgacgtcg tccgatcacg
     1261 tcctggacgg cgaaactgcg gcgttgcagg acaccaacga ccacttcctg gccaatctgc
     1321 agcgcaacgg cacctattcg gtggtgccgc ggctgcccgg cggcgagatc acccccgaaa
     1381 agctgatcgc gatcggcgaa gtggctcgcg actttgggct ctacaccaag atcaccggcg
     1441 ggcaacgcat cgacatgttc ggcgcccgcg tcgaacaact gccgctgatc tggaagcggc
     1501 tcatcgacgc cggaatggaa tccgggcacg cctacggcaa ggctctgcgg accgtgaaaa
     1561 gct
//
LOCUS       DASWBF010000246         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_417900,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000246 DASWBF010000000
VERSION     DASWBF010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417900"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1713
                     /locus_tag="VF874_08945"
     CDS             169..1713
                     /locus_tag="VF874_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9176199.1"
                     /translation="MDIDADYVIVGTGSAGSVLANRLSADPNARVTVLEAGPKDDDKF
                     VRIPAAFSKLFRSQRDWDYLTEPQKELDGRQIYWPRGKALGGSSSMNAMMWIRGFAAD
                     YDEWGQLAGEQWSFAKLAPYFARIEAGPLVISPQRSPRASTVAWLAAVQESGYRAEQP
                     NTDAPQGFCQTLVTQRRGARWSCADAYLKPALRRPNLTLLTEAHARRVLFDGARATGV
                     AFDKDGRRHVVRAAREVVLSCGAVNSPQLLMVSGIGDPSELRGHGIDVVHASPDVGKN
                     LIDHLVTPLGFEVRHDSLFAAEKPWELVKYLLRRRGMLTSNVGEAYGFVRSRPELELP
                     DLELIFAPAPFFDEGLGDPYGHAVVCGPILLKPFSRGTISLSSADPMAKPVIDPKYLS
                     DPDGADRAAMMSGLRITAKIAEAPALKDVLGKIARPLGAHDTREETLDKALRTCSQTL
                     YHPVGTCRMGTDTASVVDPQLRVRGVDGLRVADASVMPTIIRGHTHAPAVLIGEKAAE
                     LISAHP"
     gene            1841..>2994
                     /locus_tag="VF874_08950"
     CDS             1841..>2994
                     /locus_tag="VF874_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9176200.1"
                     /translation="MTMNAKRRRAHQKLAALPGVRSVRRPVSRDGEQFDLYYVRAGRK
                     SAHPLVVIPGGPGAASIALYRGFRRRAAASGLDVIMVEHRGVGMSRHDDDGADLPSEA
                     LTIEQVVDDVVAVLDDAHVDKAIMYGASYGTYIAAGVGVRHPQRVAGMILDSPVLSAA
                     DVDVVRTAIRQVLWDGTDLETAELAPKVRQLVGNAVLTPAAGQLAAAVYGFGGPALLN
                     RQLDLLLKGRHGLWTLMDRLARLLIARKAPYRNEPDLVGRIGFRELNYGDVPDGKPLD
                     PAAAYRDFASDPVPFEAEPYNLVAEMPRFTWPTVVVSGGRDLTTPPAIADRIASLIPG
                     AALLRLPTAGHSVLDSRERAGLQIAKAVSDNRVDTLPARAAALDALPGGPG"
BASE COUNT          490 a         1008 c         1014 g          482 t
ORIGIN      
        1 cacttcatcc caatccggag accggtccag caggatcacc gtgaccaccg tggaacgcag
       61 tcgcggatcg ctttccatcg cccacgtgaa ggcgtcgctg tttcgcatga attcagtcat
      121 ttgtgccaaa cctacgacgc cagccgcgaa acgcctacac ttcgcgtggt ggacatcgac
      181 gccgactacg tgatcgtcgg gaccggttcc gccgggtccg tgctggccaa ccggctcagc
      241 gccgatccga acgctcgggt gacggtgctg gaggcgggtc cgaaggacga cgacaagttc
      301 gtccgcatcc cggccgcgtt ctcgaagctg ttccgctcac agcgggattg ggactacctc
      361 accgagccgc agaaagagct cgacggtcgg cagatctact ggccgcgcgg caaggcgctc
      421 ggcggttcgt cgtcgatgaa cgcgatgatg tggatccgcg gattcgccgc cgattacgac
      481 gagtggggcc agctcgccgg cgagcagtgg tcgtttgcga agttggcgcc gtacttcgcc
      541 cgcatcgagg ccggccctct cgtcatctca ccgcagcgca gcccacgcgc gtccaccgtc
      601 gcctggcttg ccgcagtcca ggaaagcggc taccgcgccg agcagccgaa taccgatgcg
      661 ccgcaaggtt tttgccaaac cctggtgacc caacgccgag gagcgcggtg gagttgcgcg
      721 gacgcatacc tcaaacccgc tctgcggcga ccgaacctga ccttgctcac cgaggcgcac
      781 gctcggcggg tgctgttcga cggagcccgc gctaccggtg tcgcgttcga caaggacggc
      841 cggcgccacg tggtgcgcgc cgctcgcgag gtggtgctgt cctgcggtgc agtcaacagc
      901 ccgcagctgc tgatggtctc gggaatcggc gacccctcgg aacttcgcgg ccacggcatc
      961 gacgtcgtgc atgcgtcgcc cgacgtcggc aagaacctca tcgaccacct cgtcacgccg
     1021 ttggggttcg aggtgcgcca cgacagcctg ttcgccgcgg aaaagccttg ggagcttgtg
     1081 aaatatctgc tgcgccggcg cggcatgctc acgtcgaacg tcggcgaagc gtacgggttc
     1141 gttcgcagcc gcccggagtt ggagttgccg gacctcgagc tgatcttcgc gcccgccccg
     1201 ttcttcgacg agggcctggg cgatccgtac gggcacgcgg tggtgtgcgg accgatcttg
     1261 ctgaagccgt tcagtcgcgg cacgatctcg ctgtcgtcgg cggatccgat ggccaaaccg
     1321 gtcatcgatc cgaaatatct gtccgatccg gacggcgccg accgcgcagc gatgatgtcg
     1381 ggtctgcgga tcaccgcgaa aatcgccgaa gcgcctgcgc tcaaggacgt gctgggcaag
     1441 atcgcgcggc cactcggcgc gcacgacacc cgtgaggaaa cgctcgacaa ggcgctgcgg
     1501 acgtgctcgc aaacgctgta ccacccagtc gggacctgcc ggatgggaac cgacacggcc
     1561 agcgtggtcg acccgcagct gcgggttcgc ggcgtcgacg ggctgcgggt ggccgacgcg
     1621 tcggtaatgc ccacgatcat tcggggacac acccatgcgc ccgcggtgtt gatcggggag
     1681 aaggccgccg agctgatcag cgcccaccca tagcgctcgc ggggtcagca gggacacgcg
     1741 gcgcagtcat gcccagaaga tgacccaact gaagatccgt gtgcgcgctg caaatttgca
     1801 gtctcggcga aaacgccccg acccggcaca ataacgggtg atgacgatga acgccaagcg
     1861 tcgccgcgcg caccagaagc tggccgcgct gcccggcgtg cgctcggtgc gtcgaccggt
     1921 gtcacgcgac ggggaacaat tcgacctcta ctacgtgcgc gccgggcgca agtcagctca
     1981 tccgctggtg gtcattcccg gcggaccggg ggcggcgtcc atcgcgctgt atcggggatt
     2041 ccgtcgccgc gcggccgcgt cgggccttga cgtgatcatg gtcgaacacc gcggggtggg
     2101 catgtcccgc cacgacgacg acggtgccga cctgccctcg gaggcgctga ccatcgagca
     2161 ggtcgtcgac gacgtcgtgg ccgtgctcga cgacgcgcac gtcgacaagg cgatcatgta
     2221 cggcgcctcg tacggcacct acatcgccgc gggcgtcggc gtccgtcatc cgcagcgggt
     2281 tgccggaatg atcctcgact cgccggtgct gtcggctgcc gacgtcgacg tggtgcgcac
     2341 tgcgatccga caggtgctct gggacggaac cgatttggag acagccgagt tggcgccgaa
     2401 agtgcggcaa ctggtcggca acgctgtctt gaccccggcc gccggccaac tcgcggccgc
     2461 ggtatacggg ttcggcggac cggcgctgct gaatcgccaa ctggatctgc tgctgaaggg
     2521 caggcacggg ttgtggactc tgatggaccg cctcgcccgg ctgctgatcg cgcgcaaggc
     2581 cccgtaccgc aacgagccgg acctggtcgg ccggatcggc ttccgtgaac tgaattacgg
     2641 cgacgttccc gacggcaagc ccttggaccc ggcggctgcg tatcgcgact tcgcttccga
     2701 cccagtccct ttcgaggccg aaccctacaa tctcgtcgcc gagatgccga ggttcacctg
     2761 gcccacggtc gtcgtgtccg ggggccgtga cctgaccacg cccccggcga tcgccgaccg
     2821 gatcgcgtcg ttgattccgg gggcggcgct gttgcggctc ccaacggcag ggcacagcgt
     2881 gctggacagc cgcgagcggg ccgggttgca gatcgcaaaa gccgtctcgg acaaccgagt
     2941 tgacacgttg ccggcacggg ctgcggcgct ggacgcgctg cccggtgggc cggg
//
LOCUS       DASWBF010000247         7139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_23154, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000247 DASWBF010000000
VERSION     DASWBF010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23154"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VF874_08955"
     CDS             complement(<1..803)
                     /locus_tag="VF874_08955"
                     /inference="COORDINATES: protein motif:HMM:NF023742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9176201.1"
                     /translation="MSRHTTFRFCLDPTVEQRDVLDQHAGAARFAFNQCLATVKTALT
                     QRRTDPDTDVPWSGFDLINTFNRWKTTENAGRVLAVDTGGVTEIVVTGLSWRREVCQQ
                     VFEEAAVDCGRALGAWSDSRSGKRKDKRVGFPKFKKKAGVVASFRLRNKHPEARPPAI
                     RVGDNSRPRSVTLPGIGQIGVHEDTRRLRRLLAKGRATILFATHQPPRGPLVAVAQRR
                     GRPTCILPTSTRPVTTATRAAGSASIAVCRRLWSPPPPPVTKSPVSPRP"
     gene            1044..1250
                     /locus_tag="VF874_08960"
     CDS             1044..1250
                     /locus_tag="VF874_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176202.1"
                     /translation="MCCAPVARTANRTAPTIAHIWLDNGYTGSTVAHAATTAAVTVEI
                     VSRPKPKSGFTVQPHRRVVERTNG"
     gene            1396..3063
                     /locus_tag="VF874_08965"
     CDS             1396..3063
                     /locus_tag="VF874_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9176203.1"
                     /translation="MAIPDQGDSRPATSFADLHIHPEVLRAVDDVGYESPSAVQAATI
                     PAMMAGSDVVGLAQTGTGKTAAFAMPILSKIDTSSSDTQALVLAPTRELALQVAEAFR
                     RYGAHLPRINVLPIYGGQSYGVQLAGLKRGAQVVVGTPGRVIDHLERGTLDLSRLDYL
                     VLDEADEMLTMGFAEDVERILADTPEYKQVALFSATIPPAIRRLSSKYLHDPVEVTVE
                     AKTATAENISQRYIQVAGPRKMDALTRVLEVEPFDGMIVFVRTKQATGDVAERLSARG
                     FSAAAINGDMPQAQREKTIAALKEGSVDILVATDVAARGLDVERISHVLNYDIPQDTE
                     SYVHRIGRTGRAGRSGNALLFVSPRERHLLKAIEKATRQMLTETQLPSVEDVNAQRVA
                     RFRDSITDALNAPGTDLFRGLVEEYEREHDVPMADIAAALAVQSRDGEKFLMSPEPPP
                     ERRKERPERSAKPRTTNQKMATYRIDVGKRNKIGPGAIVGAIANEGGLHRSDFGHIAI
                     HPDFSLVELPAKLPRETLKALERTRISGVLINLQPDRKPGGKPRRKS"
     gene            3063..4205
                     /locus_tag="VF874_08970"
     CDS             3063..4205
                     /locus_tag="VF874_08970"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9176204.1"
                     /translation="MTLSRDDLDAQGGLESVSKAERVASLTGIRAVAALLVMGTHAAY
                     TTGKYPQGYWGTFYSRMEIGVPIFFVLSGFLLFGPWVRALAEDRPPPSLARYAWHRVR
                     RIMPAYVITVVVAYVVYHFRTAGPNPGHTWTGLFRNLTLTQVYTDNYLYSYLHQGLTQ
                     MWSLAVEVAFYVVLPLMAYLLLAVLCRRRWQPRLLLAGLVALSLITPLWLVLVHTTDW
                     LPDGARLWLPTYLVWFTGGMILAVLAVMGRQWYAAVCLPLALVCYLIVSTPLGGEPTT
                     SPRELWEALAKVLLCAVIAVLMVAPLALGNRGWYARALASRPMVWLGEISYEIFLIHL
                     IVMEVAMAEVLHWRVYTGNMVTLFVVTLAMTIPLAWLLHRFTRVRS"
     gene            complement(4186..4776)
                     /locus_tag="VF874_08975"
     CDS             complement(4186..4776)
                     /locus_tag="VF874_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9176205.1"
                     /translation="MMARDWLAASRSEVAADRILDAAAALFAERDAAAVGMHEIARAA
                     GCSRATLYRYFENREALHTAFVNREAYRLFGELGDQIKDVAAPAERLVAGVTTALRAV
                     RGNPALASWFATTEPPIGGELAELSEVIKVLTAAFLNSLGPDDPAVLERRARWLVRVI
                     TSLLIFPGADEADERQMLQEFVVPMVVPQHARTGRG"
     gene            complement(4773..6005)
                     /locus_tag="VF874_08980"
     CDS             complement(4773..6005)
                     /locus_tag="VF874_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9176206.1"
                     /translation="MTAALSHPVAFRLATAETWPNPWPMYAALRDHDPVHHVVPDNPN
                     HDYFVLSRHADVWAAARDHETFSSARGLTVNYGELELIGLQDNPPMVMQDPPVHTEFR
                     KLVARGFTPRQVEAVEPGVRTFVVERLERLLANGGGDIVAELFKPLPSMVVAHYLGVP
                     EEDRTQFDGWTEAIVAANTAEGGVGGALASVGNAVGSMMAYFTELIERRRHEPGEDTI
                     SHLVAAGVGADGDIAGTLSVLAFTFTMVTGGNDTTTGMLGGAVQLLHQRPDQRRLLAE
                     HPELIPDAVDEFLRLTSPAQCLGRTVTRDVTLHDTTIPEGRRVLFLYGSANRDERQYG
                     PDAGELDVTRRPAKILTFSHGAHHCLGAAAARMQSRVTLTELLSRCPDFEVDEDAVVW
                     AGGSYVRRPLSVPFTVTG"
     gene            complement(6119..>7139)
                     /locus_tag="VF874_08985"
     CDS             complement(6119..>7139)
                     /locus_tag="VF874_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9176207.1"
                     /translation="VKRAVAEHGLWYPPDPSSYEICSIGGNIATNAGGLCCVKYGVTT
                     DYVLGLQVVLADGTAVRLGGPRLKDVAGLSLTKLFVGSEGTLGVITEVTLRLLPKQHR
                     SSTVVATFASLEAAVNAVLAVSSRMRPSMLEFMDSVAVNAVEDKLKMGLNRDAAALVI
                     AGSDERGRSAGEDAEVIAAVFAENGATEVFSTDDPDEGEAFVAARRFAIPAVEAKGTL
                     LLEDVGVPLPRLGDLVTGIADIAAERDVMISVIAHAGDGNTHPLIVYDPSDSAMKERA
                     ELAFGEIMDLAINLGGTITGEHGVGRLKRPWLAGQIGPDAMALNRRIKDALDPAGILN
                     PGAAI"
BASE COUNT         1187 a         2473 c         2287 g         1192 t
ORIGIN      
        1 tttgggcgcg gtgatacagg cgacttggtc accggcggcg gtggcggcga ccacaaacgc
       61 cgacagaccg cgatcgacgc cgacccagcc gccctggtcg ccgtcgtcac cgggcgggtg
      121 ctggtgggca ggatgcaggt cggccggcct cgacgttgag cgacagccac cagcggcccg
      181 cgtggtggct gatgggtggc gaacagaatt gtggccctgc ctttggcgag caggcggcgc
      241 aaccggcggg tgtcctcatg caccccgatc tgcccgatac cgggcagcgt gaccgaccgc
      301 ggccggctgt tatcgccgac gcggatcgcc ggcgggcggg cttcggggtg cttgttgcgc
      361 agccgaaacg acgccaccac accggctttc ttcttgaact tggggaagcc gacccgctta
      421 tctttgcgct tgcccgagcg ggaatccgac caggcgccca gcgcccggcc gcaatccacg
      481 gcggcttcct cgaacacctg ctggcacact tcacgccgcc acgacaagcc ggtcaccacg
      541 atctcggtga caccaccggt atccacggcc aagacccggc ccgcgttctc ggttgtcttc
      601 cacctgttga acgtgttgat cagatcaaac ccgctccacg gcacatcggt gtccggatcg
      661 gttctccgtt gggtgagcgc ggttttcacc gtcgcaaggc actggttaaa cgcaaaccgg
      721 gccgccccgg catgctggtc cagcacatcg cgctgctcga cggtgggatc gagacagaac
      781 ctgaatgtgg tgtgccgaga catcaacggt gagcatccca ccacccaccg acaacaccct
      841 gacctggcgt aaacgtgtcg ggccgcgtga tcaccatcga taatcatgtg acccgaaccg
      901 accgcgccga cggcgccgac gctacgactt cgacctgtcc gacacctggg cgcacatcgc
      961 acggttcatt gcgccgaatc atccacgcca gggtcggccg tgcccacctg agcgctggca
     1021 ggagtacttc gacgccatcc cgtatgtgct gcgcaccggt tgcgcgcacg gccaaccgca
     1081 ctgcgccgac catcgcgcat atctggctgg acaacggcta caccggatcc accgtcgctc
     1141 acgctgccac cacagccgcg gtgacggtcg aaatcgtgtc ccggcccaaa cccaagagcg
     1201 gcttcaccgt ccaaccacac cggcgggtgg tcgaacgcac caacggctga atcaaccact
     1261 gcccacgccc cgacccggca ctacaagatc accctcgacg ctcacgaagc cttcctgatc
     1321 ctcagccaaa tcgccctcct actcccacga cgcgatccca accagttgtt tgacacgctt
     1381 tagactgaac acccgatggc cattccggat cagggcgatt cgcgccctgc cacttctttc
     1441 gccgacctgc acatccaccc cgaggtgttg cgggctgtcg acgacgtcgg ctacgagtcg
     1501 ccgtcggcgg tccaggccgc gacgatcccg gcgatgatgg cgggctccga cgtcgtcggc
     1561 ctggcgcaga caggcaccgg aaagaccgcc gcgttcgcga tgccgatcct gtcgaagatc
     1621 gacacctcga gctccgacac gcaggcgctg gtgctggcgc ccacccgcga actggcgttg
     1681 caggtcgccg aggcgttccg ccgctacgga gctcatctgc cgcgaatcaa cgtgctgccg
     1741 atctacggtg gccagtccta cggtgtgcag ctcgcagggc tcaaacgggg cgcacaggtg
     1801 gtggtgggca ctcccggacg ggtgatcgac cacctggagc gcggcaccct cgacctgtcg
     1861 cggctggact acctggtgct ggacgaagcc gacgagatgc tgacgatggg cttcgccgag
     1921 gacgtggagc gcatcctcgc cgacaccccc gagtacaagc aggttgccct gttctcggcc
     1981 accattccgc cggccattcg aaggctcagc tcgaaatacc tgcacgaccc cgtcgaagtc
     2041 actgtcgaag cgaaaaccgc taccgccgag aacatttcgc agcggtacat ccaggtggcc
     2101 ggcccccgca agatggacgc gctcacgcgg gtgctcgagg tcgagccgtt cgatggcatg
     2161 atcgtgttcg tccgcaccaa gcaagccacc ggggatgtcg cggaaaggct gagcgcgcgc
     2221 ggcttttcgg cggcggccat caacggcgac atgccgcagg cccagcggga gaagaccatc
     2281 gccgcgttga aggagggcag cgtcgacatc ttggtcgcca ccgacgtcgc ggcccgcggc
     2341 ctggacgtgg agcgcatttc gcacgtgctc aactacgaca tcccgcagga cacggagtcc
     2401 tacgtgcacc gcatcggccg caccgggcgc gccgggcgca gcggaaacgc gctgctgttc
     2461 gtgtcgccgc gggaacgcca cctgctcaag gcaatcgaga aggcaacccg ccaaatgctc
     2521 accgagacac agctgcccag cgtcgaagat gtcaatgcgc aacgggtggc caggttccgc
     2581 gactcgatca ccgacgcgct caacgcgcct ggcaccgacc tcttccgtgg cctggtcgag
     2641 gagtacgaac gcgagcacga cgtgccgatg gccgacatcg ccgcagcgct ggcggtgcag
     2701 tcgcgcgacg gcgagaagtt cctgatgtca cccgagccgc caccggagcg ccgcaaggag
     2761 cgacccgaac ggtcggccaa gccgcgcacg acgaaccaga agatggcgac ctatcgcatc
     2821 gacgtcggca agcgcaacaa gataggcccc ggcgccatcg tgggggccat cgccaacgag
     2881 ggcggcctgc accgcagcga cttcggccac atcgcgatcc atccagactt ctccctcgtc
     2941 gaacttccgg cgaagctgcc ccgcgaaacc ctcaaagcgc tggaacgcac ccgcatttcg
     3001 ggtgtgctga tcaacctgca gcccgatcgc aagccggggg gtaagcctag gcggaagtcc
     3061 tgatgaccct gtcccgcgat gacctcgacg cacagggcgg actcgagtcg gtatcgaagg
     3121 ctgaacgggt cgcgtcgctt accgggatcc gcgccgtcgc cgcgctgttg gtgatgggca
     3181 cgcacgccgc ctacaccacc ggcaagtatc cccagggtta ttggggaacg ttctactccc
     3241 gcatggagat cggtgtgccg atcttctttg tgctgtccgg ctttctgcta ttcggtccgt
     3301 gggtgcgcgc gctggccgag gaccgtccgc ccccgtcgct ggcccgctac gcgtggcacc
     3361 gggtgcgccg catcatgccg gcctatgtga tcacggtcgt cgtcgcgtac gtcgtgtatc
     3421 acttccgcac cgccggcccc aatccggggc acacctggac gggtctcttc cggaacctga
     3481 cgttgaccca ggtttacacc gacaactatc tgtattcgta tctgcaccaa ggacttacgc
     3541 agatgtggag cctggcggtg gaggtcgcgt tctacgtggt gctgccgctg atggcttacc
     3601 tgctgctggc ggtgctgtgc cggcggcggt ggcaaccgcg tctgctcctg gccggccttg
     3661 ttgcattgag tttgatcaca ccgttgtggc tggtgctggt gcacaccacc gactggctgc
     3721 ccgacggcgc aaggctctgg ctgccaacat atctcgtgtg gttcaccggc ggcatgatcc
     3781 tggcggtgct ggccgtcatg ggccggcagt ggtacgccgc ggtctgcttg ccgctcgcgt
     3841 tggtgtgcta tctcatcgtg tcgaccccgc tcggcggcga gcccaccacc tcgccgcgag
     3901 aactgtggga agcgttggcc aaggtgttgc tctgcgcggt gatcgcggtg ctgatggtgg
     3961 cgccgctggc gctcggcaat cgcgggtggt atgcgcgggc gctggccagt cggccgatgg
     4021 tgtggctggg cgaaatctcc tacgagatct tcctgatcca cctcatcgtg atggaagtcg
     4081 cgatggcgga ggtgctgcac tggcgggtct acaccggcaa catggtcacg ttgttcgtcg
     4141 tgaccctcgc gatgacgatc ccgttggcgt ggcttctgca ccgcttcacc cgcgtccggt
     4201 cctagcgtgc tggggcacca ccatcgggac gacgaactcc tgcagcatct gccgctcgtc
     4261 ggcctcgtcg gcgccaggga agatcaacag cgacgtgatc acgcgcacga gccaccgggc
     4321 ccggcgttcg aggacggcgg ggtcgtcggg acccagcgag ttcaggaacg cggccgtcag
     4381 caccttgatc acctcggaca gctcggccaa ctcgccgccg atcggcggct cagtggtcgc
     4441 aaaccacgac gccagggcgg gattgccgcg aaccgccctc agcgcggtgg tgacgccggc
     4501 gacgagtcgc tcggcagggg ctgctacatc cttgatctgg tcgccgagtt caccgaacag
     4561 ccgatacgcc tcacggttga cgaaggcggt gtgcaacgcc tcgcggtttt cgaagtagcg
     4621 gtatagcgtt gctcgtgaac agcctgccgc ccttgcgatt tcgtgcattc ctactgcggc
     4681 cgcgtcgcgc tcggcgaaca gcgccgccgc cgcgtcgagg atgcggtcag cggcgacctc
     4741 gctgcggctc gcggcgagcc agtcgcgggc catcagcccg tcaccgtgaa cggcactgac
     4801 agcgggcgtc tcacgtagct gccacccgcc cagacaaccg cgtcctcgtc cacctcgaaa
     4861 tccgggcagc gagagagcaa ttcggtcagt gtgacccgcg actgcatccg ggctgccgcg
     4921 gcgcccaggc agtggtgggc gccgtggctg aatgtcagga tcttcgcggg acggcgtgtg
     4981 acgtcgagtt cgcctgcgtc ggggccgtat tggcgttcgt cgcggttggc cgacccgtac
     5041 aggaacagca cccgccggcc ctcggggatc gtcgtgtcgt gcagcgtgac atcgcgggtg
     5101 acggtgcggc ccaggcactg tgcgggtgac gtcagccgga ggaattcgtc gacggcatcg
     5161 gggatcagct ccggatgctc ggcgagcaac cgacgttggt cgggacgctg gtgcagcagt
     5221 tgcactgcgc cgccgagcat gccggtggtg gtgtcattgc cgccggtgac catcgtgaac
     5281 gtgaaggcca gcaccgacag ggtgcccgcg atgtcgccgt cggcgcccac cccggccgcg
     5341 accagatgtg agatcgtgtc ttcgccgggc tcgtgacgcc ggcgctcgat cagttcagtg
     5401 aagtaggcca tcatcgaccc caccgcattc ccgaccgatg ccagcgcgcc gccgacgcct
     5461 ccctcggcgg tgttggccgc gacgatcgcc tcggtccagc cgtcgaactg cgtgcggtcc
     5521 tcttccggca cgcccaagta gtgcgcgacg accatcgacg gcagcggctt gaacagctcg
     5581 gccacgatgt cgccgccgcc gttcgccagc agccgctcca ggcgctcgac gacgaatgtc
     5641 cgcactcccg gctcgaccgc ctccacctgc cgcggggtga agccgcgcgc caccagcttg
     5701 cgaaactcgg tatgcaccgg cgggtcctgc atcaccattg gcggattatc ttgcagccca
     5761 atcagttcca gctcgccgta gttgaccgtg aggccccgcg cggacgagaa cgtctcgtgg
     5821 tcgcgcgccg cggcccacac gtcggcgtgc cgcgacaaca cgaaatagtc gtggttgggg
     5881 ttgtccggga ccacgtgatg gacggggtcg tggtcgcgca gggccgcata catcggccac
     5941 gggttgggcc aggtctcggc ggtggccaac cggaacgcca cgggatgaga caaagcagcc
     6001 gtcatgtctt attcgtacga cactgcggcg ctgactgtca accaccacac cgccgaaact
     6061 gtattccggc aggctcccac tcgcacttct gctgccaatc tgcagcctcg gcgaaaagct
     6121 agatcgccgc gcccgggttc aaaatccccg ccgggtccag cgcatccttg atgcggcggt
     6181 tcagcgccat cgcgtccggc ccgatctgtc ccgccagcca cgggcgcttg agccgcccga
     6241 cgccatgctc gccggtgatc gtgccgccca ggttgatcgc caggtccatg atttcgccga
     6301 acgccagctc ggcgcgctcc ttcatggccg agtcgctggg gtcgtacacg atcagtgggt
     6361 gggtgttgcc gtcgccggcg tgcgcgatca ccgaaatcat cacgtcccgc tcggcggcga
     6421 tgtccgcgat gcccgtcacg agatcaccga gccgcggcag tggcacaccg acgtcttcga
     6481 gcagcagcgt gcctttggcc tcgaccgctg gaatcgcgaa ccggcgcgcg gcgacgaacg
     6541 cctcgccctc gtcgggatcg tcggtggaga agacttctgt cgcaccgttt tccgcgaata
     6601 ccgcggcgat cacctcggcg tcctcgccgg cggaccggcc gcgctcgtcg gaaccggcga
     6661 tcaccaaggc tgccgcgtcg cggttcaatc ccatctttag cttgtcttcg acggcgttca
     6721 ccgcaaccga gtccatgaac tccagcatcg acgggcgcat gcggctggac accgcgagca
     6781 ccgcgttgac ggcagcctcg agggacgcaa aggtcgcgac cacggtgctc gaccgatgtt
     6841 gcttgggcag tagtcgcaat gtcacctcgg tgatcacccc gagcgtgccc tcgctgccga
     6901 cgaacagctt cgttaggctc aggcccgcaa catctttcag ccgcggcccg ccgagccgca
     6961 ccgccgtgcc gtcggccagc accacctgca agccgagtac gtagtcggtg gtgacgccgt
     7021 acttgacgca gcacagcccg cccgcgttgg tcgcgatgtt gccgccgatg ctgcagatct
     7081 catacgacga cgggtccggc ggataccaca gcccgtgttc ggcgacagcg cgtttcacc
//
LOCUS       DASWBF010000248         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_85166, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000248 DASWBF010000000
VERSION     DASWBF010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85166"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VF874_08990"
     CDS             complement(<1..629)
                     /locus_tag="VF874_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176208.1"
                     /translation="MSAAAGWVLGVDFGVVNTAAALRDPSGRVSELRLSSTGVLMPSA
                     VFCPEQQLLVGDGALRAGLSDPGALVPYPKRRLADGMRCKLAVAGQQAYVPGLVAAVF
                     ADVRKRAREATGGDLPQDLMLSYPDIHHASFAETSQRRDGLQEAADLLGCRRCQLVSD
                     STAVTGLAQSEAQLVSPALLVVNFGAVRCDAVVCTRQYDGGYAAVAARSV"
     gene            963..2213
                     /locus_tag="VF874_08995"
     CDS             963..2213
                     /locus_tag="VF874_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KasA/KasB family beta-ketoacyl-ACP synthase"
                     /protein_id="HEX9176209.1"
                     /translation="MAALSTGDGFAGVVVTAVASTTALAPDAEETWQQLLEGRSGIRT
                     LDKPFVEEFKSAVRIGGVLREDFDEHLTRVELRRLSFMQKMSTVLGRRLWDTAGSPEI
                     DTKRLLVSITQALATTETLVFLYDDWKARGMRAASPLAVQMHMPNAPAAAVGLDRGAK
                     AGIIAPVLADASGAAAIAHAWQNIVLGEADIAICGGVEARIEAVPVAAFTQLGILSTN
                     NDDPPGACRPFDKDRDGMVFGEGGALLLIETEQHAKTRGAPILARLMGAAITSDCYDV
                     IKPDPSGERAGDAIAHAIELAGLSPTDIDHVNAHATGTTFGDLAEAHAIRHALGNHTP
                     AVYAPKAALGQSMGAAGAVEAVLTVQALRDGVVPPTLNLKTLDPEIDLDVLAGGPRRG
                     TYRYALSNSMALGGHNVALVFGAY"
     gene            2434..>2983
                     /locus_tag="VF874_09000"
     CDS             2434..>2983
                     /locus_tag="VF874_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176210.1"
                     /translation="MGTPVYVTLLVAAIAGVVALSVGWFARITARDIAALTANQKRQD
                     VRIKALGAYVDLLMSGAAATQAYVWYADPEFVRNKEITQENWDSARELFQPAYEAYSK
                     VKYLGTALADESLRETYDRFVTAPHSVIFAKESVKDAWHRIVRAEGQPDSIAAAITAA
                     NDLYKKALADYPTEVPNSITKRG"
BASE COUNT          561 a         1027 c          930 g          465 t
ORIGIN      
        1 acgctgcggg ccgctactgc ggcgtagcca ccgtcatact ggcgggtgca caccaccgca
       61 tcgcatctca ccgcgccgaa attgaccacc agcagcgccg gcgaaaccag ctgcgcctca
      121 ctttgcgcca acccggtgac cgccgtcgag tcggacacca gctggcagcg ccggcacccg
      181 agcaggtctg ccgcctcctg taggccatca cggcgctgcg acgtttcggc gaacgatgcg
      241 tgatgaatgt cgggatagga aagcatcaaa tcctggggaa gatcgccacc ggtcgcctcc
      301 cgtgcccgct tccggacatc agcgaacacc gcagcgacca aacctgggac gtaggcctgc
      361 tgtccggcta ccgccaattt gcatctcatg ccgtcggcca gccggcgttt cgggtaaggc
      421 acaagggcgc caggatcgga caacccggcc cgaagcgcac cgtcgcccac caacagctgc
      481 tgttctgggc agaacacagc agagggcatc aacaccccgg tgctcgacaa ccgcagttcg
      541 ctcacccgac ccgacggatc acgaagcgca gcagcggtgt ttaccacgcc gaagtcgacg
      601 cccagcaccc agccggctgc cgcactcaac tcatcctccc ctcccgacca gctttcattg
      661 tcaaccaaac ggcggctgga acgcacagtt ccaccggcag aagctcggcc aacctcgaca
      721 cggcgacggt aaaccgcggt gagtcgggcg gttaatgaat cgggcctcag tccgcggggc
      781 gcgccgtccg cggtcatgat gggcgcaacg actttcaccg cccatgtgcg cagcggcccg
      841 atacccggca cgcaactgac cgaacgcgag gtgcctcggt gcctggggca gcacgttttt
      901 ggggtatggt ccggcaaggg ctgcaacgaa atacctggtg agaccagcaa cggagcggag
      961 gcatggcagc gttgagcacc ggcgatgggt tcgccggcgt cgttgttacc gcggtcgcat
     1021 cgacaactgc tctggcgccc gacgccgagg aaacgtggca gcagctgctg gagggccgca
     1081 gcgggatccg cacactcgac aagccgttcg tcgaagagtt caagtccgcg gtgcgcatcg
     1141 gcggtgttct gcgtgaggac ttcgacgagc acttgacgcg tgtcgagctg cgtcggctgt
     1201 cgttcatgca gaagatgtcc acggtgctgg gccggcggct ctgggacacc gccggctcgc
     1261 cggagatcga caccaaacgg ctcctggtct cgatcacgca ggcgttggcc accaccgaga
     1321 cgctggtctt cctctatgac gactggaagg cgcgcggcat gcgcgcggcc agcccgctgg
     1381 ccgtgcagat gcacatgccc aacgcgcccg ccgccgcggt cgggttggat cgtggagcca
     1441 aggccgggat catcgccccg gtgctggccg acgcctccgg cgcagcggcc atcgcccacg
     1501 cgtggcaaaa cattgttctc ggcgaagccg acatcgcgat ctgcggcggc gtcgaagccc
     1561 ggatcgaggc cgtgcccgtg gcggcgttca cccagctggg catcctgtcg accaacaacg
     1621 acgacccgcc cggcgcctgc cgccccttcg acaaagatcg cgacggcatg gtgttcggcg
     1681 aaggcggcgc gttgctgctc atcgaaaccg agcaacacgc caagacgcgc ggcgccccaa
     1741 tcctggcccg cctgatgggg gcggccatca catccgactg ctacgacgtc atcaagccgg
     1801 accccagcgg cgagcgggcc ggcgacgcca tcgctcacgc gatcgagctg gccggcctga
     1861 gcccgaccga catcgaccac gtcaacgccc acgccaccgg aaccacgttc ggcgacctgg
     1921 ccgaagccca cgccatccgc cacgctctcg gcaaccacac gccggcggtg tatgcgccca
     1981 aagcggcgct gggccagtcg atgggcgccg ccggagcggt ggaggcggtg ctgacggtcc
     2041 aggcgctgcg cgacggcgtc gttccgccga ccctcaacct gaaaaccctc gacccggaaa
     2101 ttgaccttga cgtgctggct gggggtcccc gccgcggcac gtatcgctac gcgctctcga
     2161 attccatggc cctcggcggc cacaacgtcg ccctggtatt cggcgcgtat tgacccgccc
     2221 cgtggctagc cacttgagca aacaccaacg ccaccaacaa caaattgtcg atggtggatc
     2281 ctcaagcggc tagacaccga ccgtgctgct tgaccggatg aatgggaggt gtttcgcaac
     2341 ggcttcctcg ccatcactcc tggtgctcgg ctcacgtcga cacgacgcaa aggtgtcggt
     2401 gtagtcgcac gattgttcga tacagtgccg tccatgggta ctccggtgta cgtcactctt
     2461 ctcgtcgcag cgatcgcagg agtggtagcg ctgtctgtcg ggtggttcgc tcggatcacg
     2521 gcgcgggaca tcgcagcgct gaccgctaac cagaaacgtc aggatgtccg gataaaggcg
     2581 ctaggtgcgt acgtcgatct gctgatgtcg ggtgctgctg cgacccaggc atacgtttgg
     2641 tacgccgatc cggagttcgt ccgcaacaag gagatcaccc aggaaaattg ggacagcgcc
     2701 cgcgagctgt tccaacctgc ttacgaggcg tattccaagg tgaagtacct cggcaccgct
     2761 ctcgccgacg agtcgctgcg ggagacctat gaccggtttg tcaccgcccc gcactcggtg
     2821 atcttcgcca aagagtcggt aaaggacgca tggcaccgca tcgtccgagc ggagggtcag
     2881 cccgactcca tcgccgctgc gatcaccgcc gccaacgacc tctacaagaa agcgttagcc
     2941 gactatccga ccgaagtgcc caactcgatc acgaagcgtg gga
//
LOCUS       DASWBF010000249        13212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_224700,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000249 DASWBF010000000
VERSION     DASWBF010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224700"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VF874_09005"
     CDS             complement(<1..305)
                     /locus_tag="VF874_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX9176211.1"
                     /translation="MAGSPFGPYELVQSLGRDGIGDVWRAHDTVTDRMVVIKVLPPQF
                     AQDEDFVQRFRREAELATRLNEPHAVPIHTYGEIDGRLYVDTRLIEGRTLETVLREGP
                     "
     gene            397..1470
                     /locus_tag="VF874_09010"
     CDS             397..1470
                     /locus_tag="VF874_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9176212.1"
                     /translation="MAIARPAPMEAGGTFRGRSRVQPAQMPAVELLERAAQDVELPQS
                     PNTIVIVDYGACAGHNSLLPIGAAISIVRNRAEASRPICVFHADVAENDYAGLFESLE
                     TDPQSYLRDDPATFAAAIGPSVYRQVVPTGLVTLGWSAWAVHWLSRVPAPIPDHVHIE
                     LSRDTDARAAYARQAADDWIAFLTARSREMVPGGRLVVLTMAHDDGDCSYQPLLEAIV
                     EELAAMVAEGVVSRDEVARMAVPSIGRSEVELTAPFAPKNRFAGLTVEHLELFDAEDQ
                     YWTKFQKDRDATAFATNWSAFVRASVFPTLLTALEGGDKGPDAERFVERLETGLRGRL
                     ADDPKRVRLPLAKLSLHKSAWPR"
     gene            complement(1477..3309)
                     /locus_tag="VF874_09015"
     CDS             complement(1477..3309)
                     /locus_tag="VF874_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX9176213.1"
                     /translation="MAEAIGLSIGATRFAGVQLGHAPVTRQSVLTLYGQRSPELGVPG
                     ENPNLTQPGVVVTGFVERVGDPVGMVAGDGAMHRGEFLVADALRELFYTVTGGRPPAQ
                     PPTVTYPAHWRPATVDSLRTALTQVPEWSRRGQVTLVSDAAAAISALQSDPGVPARGV
                     IALCDFGGTGTSITLLDASRGYAPVGPTVRHTDFSGDLIDQALLTHVVTDLSAAASVD
                     ATGTSALGPLHRLRGQCRGAKERLSGTTVTALNAELPGFRGEVRLTRAELEEQIRRPL
                     ADLLGVVQDTIDRSGVRLGDLVAVASVGGGARIPLVTAMLSERFRVPVITSPRPELAA
                     AGGAALRAAWGPADDKATVIAPAVAATAMAPAAFADADAASTTFRALAWSEAHDVPPL
                     VPAVEPYDEGEPAPTGLSGARPQFEFDAAEAPAVAGVPWYRRRSSWLGVAASVVLLLG
                     TSAVVAFGSDSTPASGTSPLPPSTTQAAPGVAAAPPAESPAQPQQDSPLRTVYAVPAP
                     ATEYQAPAQQQAAAPPPQAAAPAPAAPAPSSEAPPPPPAETTTVTSTTQAPPSTTTVT
                     ATPTAQQPPSEPTQAPPPSTQPPVTFTLPPLPSIPGLFAPQGNR"
     gene            complement(3389..4459)
                     /locus_tag="VF874_09020"
     CDS             complement(3389..4459)
                     /locus_tag="VF874_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEX9176214.1"
                     /translation="MTKRIVLWGTGFVGRMVIPEIVKHPLFELVGVGVSNPDKVGRDA
                     GEICGIAAQGVAATDDVDALIELGPDALVHYGPTAAHAGDNIRLITRFLRAGIDVCST
                     AMTPWVWPTADLNPPNWIVPITEACELGESSCFTTGIDPGFANDLFPMTLMGLCSEVR
                     KVRASELLEYTNYEGDYDNEMGIGRPPEFTPLFENSDILVFAWGPTVPMIAHAAGIML
                     DEITTTWEKWVTPTERKTAKGVIAPGNVAAVRFTINGVYRGETRIQLEHVNRIGHDAA
                     PDWPSGNQDDVYRVDIEGTPSIFQETAFRFTDGSGRDAAAAGCLATGLRALNAVPAVN
                     DLAPGWVTALDLPLIPGAGTIR"
     gene            4558..5841
                     /locus_tag="VF874_09025"
     CDS             4558..5841
                     /locus_tag="VF874_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9176215.1"
                     /translation="MGIATRINGQAPPDVPLSEIDLGTWDFWAQDDDFRDGAFVTLRR
                     EQPISFWEIPKQEDYDAGKGHWALARYDDVHYVSRHPQIFSSVPSITIGDNIPELSEF
                     FGSMIVTDDPRHQRLRNIVSRAFTPRVVARTEESVRDRARRLVDAMITNHPDGKGEVV
                     SELAGPLPLQVICDMMGIPEEDNDTVFHWTNVILGFGDPDLASELNVFIKASMDFGAY
                     ATALADDRRANPRDDLTTSLVQAEVDGEKLTSSEIASFFMLLAIAGNETTRNAISHGV
                     LALTRYPDEREKWWSNFDAVCHSAVEEIVRWASPVVYMRRRVTQDTELGGVKLAAEEK
                     VSMWYCSANRDEAKFANPYTFDVTRDPNPHVGFGGGGAHFCLGANLARREIAVMFEEL
                     HHRIPDLVATEEPARLLSAFIHGIKQLPVAWTPPS"
     gene            complement(5848..6258)
                     /locus_tag="VF874_09030"
     CDS             complement(5848..6258)
                     /locus_tag="VF874_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176216.1"
                     /translation="MKKLLAVAGGSVALASMGFLTAGHASSDNGAADVSGLAYAQAVK
                     VLKSQGYKAVFGGSVGSDVPQARCIVESQKFLPNARVSLMLNCTKAAQPAPSAADPNA
                     PVVGSNGITTVKATPVAPPPTELAPAPPPLMPEG"
     gene            6430..7194
                     /locus_tag="VF874_09035"
     CDS             6430..7194
                     /locus_tag="VF874_09035"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX9176217.1"
                     /translation="MLIGSHVSPEDPLAAADAEGADAVQIFLGNPQSWKAPKPRADAA
                     RLRESPLPVYVHAPYLINVASPNNRVRIPSRKILQQTCDAAADIAAAAVIVHGGHVTA
                     DDDDLKAGFERWRKALEQLNTTVPVYLENTAGGDHAIARHFDTIARLWDYIGDQGIGF
                     CLDTCHAWAAGEELIDAVEQIKAITGRIDLVHCNDSKDPHGSGRDRHANLGTGEIDVD
                     LLVAVVTAADAPVICETADQGRKDDIALLRDRTAAQ"
     gene            complement(7202..7714)
                     /locus_tag="VF874_09040"
     CDS             complement(7202..7714)
                     /locus_tag="VF874_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176218.1"
                     /translation="MRIVRVLIAAVFAAAVAVGTANPATARPVDEPMYGIYTYHQDGA
                     PEEQWTIYATCVQAGCVLHMSSIVSRSLGPNSDTPGYGGDARKVNALWTWQVKKEKGL
                     RCPDGTWAPSNYQYQWDQATLAGTLTILTTDTCASTPGATNYPFTLTFKEPLPVPIIL
                     DPLNQIENLW"
     gene            complement(7756..8820)
                     /locus_tag="VF874_09045"
     CDS             complement(7756..8820)
                     /locus_tag="VF874_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEX9176219.1"
                     /translation="MLTGKLADVTGPNITEQWGVTCTDLGASVLAPNGKLVSVFGDTF
                     SGRRVGEGDWRSPVVLIGTGDANNEIVYEHAGGPDPDYARQLWPYIHDDASTGWTRGG
                     ISTVIPSDLLRVDDSLYLHAIVNHGFGNVMWSEIWKSDDSGVSWTHMGEGVKFPGNLH
                     GGHAQCWSWDYDPETQWVYVVSTGFQRDKGIILRRVRPEDIGDRAEYMGWRFAGGQWG
                     WGGGEPTPITPLGETWGELTFRRMKAGRWILGGFLSSKYSLGYRVVQSPVANMHATPI
                     QTPVVGTAWTDEDHAANKVAQLYGGYVLPASRFDIVGGFGLMVSQWHTAAGWPYRAMQ
                     FRALVKDSTIRRRPTDPIIF"
     gene            8992..9282
                     /locus_tag="VF874_09050"
                     /pseudo
     CDS             8992..9282
                     /locus_tag="VF874_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            9279..9737
                     /locus_tag="VF874_09055"
     CDS             9279..9737
                     /locus_tag="VF874_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9176220.1"
                     /translation="MSWWLCGAERTLTEDVAAPPGRVREFYVDLDNIRLVHPLVVSVQ
                     TISRTDTPNGYMQTCRVQDRIPLGLITLRTRYVARLHVPASGEVIADARQFPWVRLRS
                     VVTFGETDTGTRIVEHMRIQAPRLLAAVTIREAVEAHTVMLANIRQHFAR"
     gene            complement(9730..10227)
                     /locus_tag="VF874_09060"
     CDS             complement(9730..10227)
                     /locus_tag="VF874_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176221.1"
                     /translation="MTRHHISALVALAVASLGTSVCLAPPALADPSWNGQYEVTFFTY
                     QKSGTSMAVNQPESMHVDIYTFSSSCSSGHCVATITDGPPPRNPTIPQPVQFTWDGSA
                     WTSTSTWRWDCLFPDGTIEWSPAGSRVSYTPQPDGSLTGEMHTDISSGACQGTVDMPM
                     QALPI"
     gene            10396..11775
                     /locus_tag="VF874_09065"
     CDS             10396..11775
                     /locus_tag="VF874_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9176222.1"
                     /translation="MADVATRLAGIVGDKNLLTGNAISEDYAHDEALTEAPQKPAYVA
                     KPATADEVAELLKLATEQRLPVTARGSGCGLSGAAQPVEGGLLISFERMNAVLEVDAE
                     NHVAVVQPGVTLTQLDAKTAEVGLSYTVYPGELSSSVGGNVGTNAGGMRAVKYGVTRH
                     NVLGLQAVLPTGEIIRTGGKMSKISSGYDLTQLIIGSEGTLALATEVIVKLVPRLAHG
                     ATVLAPFDDFDQVMAAVPKIVASGLAPSILEYVDNITMAALVHRENLELGVPDEIREK
                     CEAYLVVALENRDADRLHEDAESLGELLSELGAVDAYVLEGGAARRLIEAREKVFWAA
                     KAMGVDDIIDVVVPRASMPRFLTTARELAVGAGAHALGCGHAGDGNVHVGIFCPDADI
                     RKKLLAEIFAVAMELGGAISGEHGLGRAKTSYFLELEDPAKIALLRRIKESFDPAGIL
                     NPGVLFVDA"
     gene            11808..13096
                     /locus_tag="VF874_09070"
                     /pseudo
     CDS             11808..13096
                     /locus_tag="VF874_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732327.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
BASE COUNT         2215 a         4304 c         4550 g         2143 t
ORIGIN      
        1 ggcccctcgc gcaagacggt ttccagggtg cgaccctcga tcagccgcgt gtccacatag
       61 agccgtccat cgatttcgcc gtacgtgtgg atcggaaccg cgtgtggctc gttaagccgt
      121 gttgccagct ccgcctcgcg ccggaaccgc tggacaaagt cttcgtcctg cgcgaactgc
      181 ggaggcagca ccttgatgac caccatgcgg tcggtcacgg tgtcatgtgc gcgccagacg
      241 tcgcctatgc catcgcgacc aagcgactgc accaactcgt aaggaccgaa cggagaaccc
      301 gccacctagg cattctcgcg caaacgaggc cgaaaaccgg cggctgcggg aaatctcgga
      361 gcgcctcacc ggcgctgact gactacgctg cccgacatgg ccatcgcccg gccggcgccg
      421 atggaggcgg gaggcacatt cagaggacgc tcgcgggtac aaccggcgca gatgcccgcc
      481 gtcgagctgc tcgagcgggc ggctcaagac gtcgagctgc cgcagtcgcc gaacaccatc
      541 gtgatcgtcg actacggcgc gtgcgcggga cacaactcgc tgctgccgat cggcgccgcc
      601 atttccatcg tgcgcaaccg ggcggaagcc agccgtccga tctgcgtctt ccacgccgac
      661 gtcgccgaaa acgactacgc cggactattc gaatcgctgg agaccgaccc gcagagctac
      721 ctgcgtgacg acccggcgac gttcgcggcg gcgatcgggc cgtcggtgta ccggcaggtg
      781 gtgccgaccg gccttgtgac gctgggctgg agcgcatggg ccgtgcactg gctgagccgc
      841 gtgccggcgc cgatccccga ccatgttcac atcgagttga gccgcgacac cgacgcgagg
      901 gcggcgtatg cccgtcaggc cgccgacgac tggatcgcgt tcctgaccgc ccgaagccgc
      961 gagatggtcc cgggcggacg cctcgtggtg ctcacgatgg cgcacgacga cggcgactgc
     1021 agctaccagc cgctgctcga ggcgatcgtt gaggagctgg ccgccatggt ggccgagggt
     1081 gtggtcagcc gtgacgaggt ggcgcggatg gcggttccgt cgataggacg cagcgaggtc
     1141 gagctgaccg cgccgttcgc accgaagaat cggttcgccg ggctgacggt tgaacatctg
     1201 gaactgttcg acgccgagga ccagtactgg acgaagttcc aaaaagacag ggatgcaaca
     1261 gctttcgcca ccaactggtc cgcgttcgtc cgggcgtcgg tgttcccgac cctgctcacc
     1321 gcgctcgagg gcggcgacaa ggggccagat gccgagcggt tcgtcgagcg cctggaaacc
     1381 ggcttgcgtg ggcggctggc cgacgacccc aagcgcgtga ggcttccgct cgcaaagcta
     1441 tcgctgcaca agagcgcctg gccgcgctga ccggggctat cggttgccct gcggagcgaa
     1501 caggccggga atcgacggga gcggcggaag ggtgaaggtc accggcggct gcgtactggg
     1561 cggcggtgcc tgggtgggct ccgacggcgg ctgctgggcg gtcggcgtcg ccgttacggt
     1621 cgtggtcgac ggaggcgctt gcgtcgtgga cgtcacggtg gtggtctcgg ccggcggcgg
     1681 gggcggcgcc tctgaactcg gtgcgggtgc cgccggcgct ggtgcggcgg cttgcggggg
     1741 cggcgcagcc gcctgttgct gagccggcgc ctgatactcc gttgccggtg ccgggacggc
     1801 gtacacagtg cgcagcggcg aatcctgctg cggctgcgcg gggctttccg ccggcggtgc
     1861 ggccgccact cctggtgctg cctgcgtggt cgacggtggc agcggcgagg tgcccgacgc
     1921 tggcgtgctg tcgctgccga acgccacgac cgcgctggta ccgagcagca ggacgacgga
     1981 tgcggcgaca ccgagccacg acgatcggcg ccggtaccac ggcaccccgg caacggccgg
     2041 tgcctcggcg gcgtcgaact cgaattgtgg gcgtgcaccg gacaagcccg tcggcgccgg
     2101 ctcgccctcg tcatatggct cgacagccgg aactagcggc ggcacgtcgt gcgcctcgga
     2161 ccaggccagc gctcggaatg tggtggacgc cgcgtcggcg tcggcgaagg cggccggtgc
     2221 catagcagtc gctgcgacgg ccggtgcgat caccgtcgcc ttgtcgtcgg ccggccccca
     2281 ggcggcccgc agcgcggcac cgcccgcggc ggccaactca gggcgtgggc tagtgatcac
     2341 gggcacgcgg aagcgctccg acagcatcgc ggtgacgagt gggatgcgcg cgccgccgcc
     2401 caccgacgcg acggcgacca ggtcgcccag gcgcacgccg ctgcggtcga tcgtgtcctg
     2461 cacgacgccc agcaagtcgg ccagcggcct gcggatctgc tcctcgagtt cggcgcgggt
     2521 cagccgcacc tcgccgcgga agcccggcag ctccgcgttc agcgcggtca ccgtcgtgcc
     2581 ggacagccgc tccttggcgc cgcggcactg cccgcgcaac cggtgcagcg gcccgagcgc
     2641 cgaggtgccg gtggcgtcga cggacgccgc cgcggacagg tcggtgacca cgtgggtcag
     2701 cagcgcctgg tcgatcaggt cgccggagaa gtcggtgtgt cggacggtgg ggccgacggg
     2761 cgcgtatccg cgcgacgcgt cgagcagcgt gatgctggtg ccggtgccgc cgaaatcgca
     2821 cagcgcgatc accccgcgcg ccggaacgcc cgggtcggac tgcagcgcgg agatcgccgc
     2881 cgcggcgtcg gagaccaacg tgacctgccc gcgtcgggac cattcgggca cctgagtcag
     2941 cgcggtgcgc agcgagtcga ccgtggccgg acgccagtgc gccggatacg tgaccgtggg
     3001 cggctgggcg ggcggccggc cgcccgtcac ggtgtagaac agctcccgca acgcgtcggc
     3061 gaccaggaac tcgccgcggt gcatggcccc gtcaccggca accatgccga ccgggtcgcc
     3121 cacccgctcg acgaaccccg tcaccaccac gcccggctgc gtgaggttgg ggttctcccc
     3181 cggcacgccc agttcgggtg accgctggcc gtacagcgtc agcaccgact gccgcgtgac
     3241 cggcgcgtgt cccagctgca cgccggcaaa ccgcgtcgca cctatcgaca ggcctatagc
     3301 ctcagccatc gctcccaaac ccgcttagaa aacctagaaa acaatgccct ccgatagatg
     3361 acatcgccgg cgacgcccga tcagttactc agcgaatcgt ccctgccccc gggatcaaag
     3421 gcaagtcgag cgcggtgacc catccgggcg ccaggtcgtt gaccgcgggc acagcgttca
     3481 acgcccgcag ccccgtagcc aggcagccgg ccgcggcggc gtcgcgaccg gacccgtcgg
     3541 tgaaccgaaa agcggtctct tggaagatgc ttggcgttcc ctcgatgtcg acgcggtaga
     3601 cgtcgtcttg gttgcccgac ggccaatccg gcgcggcatc atgaccgatg cggttgacgt
     3661 gctcgagctg gatccgggtc tcgccgcggt acacgccgtt gatcgtgaag cgcaccgcag
     3721 caacattgcc gggcgcgata acgcccttgg cggtcttgcg ttcggtgggt gtcacccact
     3781 tctcccaggt cgtggtgatc tcgtcgagca tgatgcccgc cgcgtgggcg atcatcggca
     3841 ccgtcgggcc ccaagcgaac accaggatgt cgctgttctc gaacaacggc gtgaattccg
     3901 gcggcctgcc gatccccatc tcgttgtcgt agtcgccctc gtagttggtg tactcgagca
     3961 gctccgaggc acgcaccttg cgcacttcac tgcagaggcc catcagcgtc atcgggaaca
     4021 ggtcattcgc gaagcccggg tcgatgccgg tggtgaagca ggacgattcg ccgagctcgc
     4081 acgcctcggt gatcgggacg atccagttcg gcgggttcag gtccgcggtc ggccacaccc
     4141 acggcgtcat cgcagtcgag cagacgtcga tgccggcgcg taggaagcgc gttatcagcc
     4201 ggatgttgtc gccggcgtgc gcggcggtcg gcccgtaatg gacgagcgca tccggcccca
     4261 gctcgatcag cgcgtcgacg tcatcggtag ccgccacgcc ctgcgccgct atcccgcaga
     4321 tctcgcccgc gtcgcggccg accttgtcgg ggttgctgac cccgacgccg acgagctcga
     4381 acaacgggtg tttgacgatc tcggggatca ccatcctgcc gacgaagccg gtgccccaca
     4441 acacgattcg cttagtcacc gccccaccat aggcgtcggc gataggctga ggctcttact
     4501 ccctgagaac ggcgatgaac gcttgcgtga agagcaaaga acgaggtagg acctcacatg
     4561 ggtattgcga ctcgaatcaa cggtcaggcc ccgccggacg tgccgctgtc cgagatcgat
     4621 ctagggacat gggacttctg ggcgcaggac gacgacttcc gcgacggcgc cttcgtcacg
     4681 ttgcgccgcg agcaacccat ctccttctgg gagataccga agcaggaaga ctacgacgcc
     4741 ggtaaggggc actgggcgct ggcccggtat gacgacgtgc actacgtcag ccgccatccc
     4801 cagatcttca gttcggttcc cagcatcacg atcggcgaca acatacccga gctgtcagag
     4861 ttcttcgggt cgatgatcgt gacggacgac ccgcgtcatc agcgccttcg caacatcgtc
     4921 agcagggcgt tcacgccacg ggtcgtggcc cggacggagg agtcggtgcg ggatcgcgca
     4981 aggcggctcg tcgacgcgat gatcacgaac cacccagacg gcaagggtga ggtcgtctcc
     5041 gagctggcgg gtccgctgcc gctacaggtg atctgcgaca tgatgggcat tcccgaggaa
     5101 gacaacgaca ccgtctttca ctggaccaac gtgatcctcg gcttcggcga ccctgacctc
     5161 gcctccgaac tcaacgtatt catcaaggct tcgatggatt tcggcgcgta cgcgacggcg
     5221 cttgccgacg accggcgcgc gaacccgcgg gacgacctga ccaccagcct ggtgcaggcc
     5281 gaggtcgacg gcgagaagct gacatcgagc gagatcgcgt cgttcttcat gctgctggcg
     5341 atcgccggca acgagaccac ccgcaacgcg atcagccacg gtgtgctggc gctgacccga
     5401 tatcccgacg agcgcgagaa gtggtggtcg aacttcgatg cggtctgcca ttcggcggtc
     5461 gaggaaatcg tgcggtgggc atcgccggtg gtctacatgc gccgccgcgt cacccaggac
     5521 accgagttgg gcggggtcaa gttggcggcc gaagagaagg tgtcgatgtg gtactgctcg
     5581 gcgaaccgcg acgaggcgaa gttcgccaat ccgtacacgt tcgacgtcac gcgcgacccg
     5641 aatccgcacg tcgggttcgg cggcgggggc gcgcacttct gcctgggcgc gaatttggcg
     5701 cgccgggaga tcgccgtgat gttcgaggag ctgcaccacc ggatacccga cctggtggcc
     5761 accgaggagc cggcgcggct gttgtcggcg ttcatccacg ggatcaagca gctcccggtg
     5821 gcgtggacgc cgccgagctg acggtgatca gccctccggc atcaggggcg gaggcgccgg
     5881 ggcgagctca gtcggcggcg gagccactgg ggtcgccttc accgtggtga tgccgttcga
     5941 gccgacgacg ggagcgttgg ggtctgccgc ggatggcgcc ggctgtgcgg ctttggtgca
     6001 gttcagcatc agcgagaccc gcgcgttcgg caggaacttc tggctttcga cgatgcaccg
     6061 cgcttgcggg acatcgctgc cgacggagcc gccgaacacg gctttgtagc cctgcgactt
     6121 caggaccttg acggcctgcg cgtacgccag cccagaaacg tcggcagccc cgttgtcgct
     6181 gctggcgtga ccggcggtga ggaaacccat ggacgccagc gcgacggatc ccccggcgac
     6241 ggctagaagc tttttcacgt gacctccgtc tgtggctgcg ctgcgaagat aacgcaagaa
     6301 cgcgggacgc gaaaaccgac aggcatggcc gacgccccgc cgttgcgtaa tcgatgcgac
     6361 gtcacgaccg ggttgtcgtg cctttccggt gaggcgttac gcgtcggtgg accggcttaa
     6421 actggcccgg tgctcattgg ttcgcacgta agcccggagg atccgctggc cgctgccgac
     6481 gcggaaggcg ccgacgcggt gcagatcttc cttggcaatc cgcaaagctg gaaggcaccg
     6541 aagccgcgcg ccgacgcggc ccggctcagg gagtcgccgc tgcccgtcta tgtgcacgcg
     6601 ccgtacctga tcaacgtcgc ctcccccaac aaccgggttc gcatcccgtc acgcaagatc
     6661 ctgcagcaga cctgcgacgc ggcggccgac atcgcggcgg ccgcggtgat cgtgcacggc
     6721 ggccacgtca ccgccgacga cgacgacctg aaggccggct tcgagcggtg gcgcaaagca
     6781 ctggagcagc tgaataccac ggtcccggtc tacctggaga acaccgccgg cggcgaccac
     6841 gcgatcgccc gccacttcga cacgatcgcc cggctctggg actacatcgg cgaccaaggt
     6901 atcggctttt gcctcgacac gtgccacgcg tgggccgccg gtgaagagct gatcgacgcg
     6961 gtcgaacaga tcaaggcgat caccggccgc atcgatctgg tgcactgcaa cgactccaaa
     7021 gacccgcatg gttcagggcg tgaccgccac gccaatctcg gtaccggcga gatcgacgtc
     7081 gacctgctcg tcgcagtggt cacggcagcc gacgcacccg tcatctgcga aaccgcagac
     7141 caaggtcgca aggacgacat cgcgctgttg cgcgaccgca cggcggctca gtagccgtcg
     7201 ctcaccacag attttcgatc tggttcagcg gatccaggat gatcggaact ggcagtggct
     7261 ctttgaacgt cagcgtgaac gggtagttgg tcgcgcccgg cgtcgacgca caggtgtccg
     7321 tggtcagaat ggtgagcgtc ccggcaagcg tggcctggtc ccactgatat tgatagttcg
     7381 acggcgccca ggtgccgtcc gggcatctca agcccttctc cttcttgact tgccacgtcc
     7441 acagcgcgtt gaccttccgc gcatccccgc cgtagccggg ggtgtcgctg ttcggcccga
     7501 ggcttcgcga gacgatgctg gacatgtgca gcacgcaccc ggcctgcacg caggtggcgt
     7561 agatcgtcca ctgctcttcg ggcgcgccat cctggtggta ggtgtagatg ccgtacatcg
     7621 gctcgtccac cggtcgggcc gtggcggggt tcgctgtacc caccgcaacg gccgccgcga
     7681 acacagctgc gatcaacaca cgaacaatgc gcaccgggca agtatcacaa cgtttgccgc
     7741 gggccgatag aggttctaga aaatgatcgg gtcggtcggc ctgcgccgga tcgtgctgtc
     7801 cttgaccaac gccctgaatt gcattgcgcg gtaaggccat ccggcagcgg tgtgccactg
     7861 ggagaccatc agtccgaacc cgccgacgat gtcgaaccgg gatgcgggca acacgtaacc
     7921 gccgtagagc tgcgcgacct tgttggcggc gtggtcttcg tcggtccacg cggtgccgac
     7981 gaccggcgtc tggatcggcg tggcgtgcat attggccacg ggcgattgca ccacgcgata
     8041 tcccagcgag tacttcgacg acaggaaccc gccgaggatc catctgccgg ctttcatgcg
     8101 gcgaaacgtc aactcacccc aggtctcccc gagcggcgtg atcggtgtcg gctcgccgcc
     8161 accccagccc cactggccgc cggcgaaacg ccagcccatg tattccgccc tgtcgccgat
     8221 gtcttctggg cgcacccgcc gcagaatgat gcccttgtcg cgctggaagc cggtggagac
     8281 cacatagacc cattgcgtct cggggtcgta gtcccaggac cagcactgcg catggccgcc
     8341 gtgcagattt cccgggaact tgacgccctc ccccatgtgt gtccacgaga ccccgctgtc
     8401 gtccgacttc cagatttcgg accacatcac attgccgaag ccgtggttga cgatcgcgtg
     8461 cagatagagg gagtcgtcga cgcgcagcag atccgacgga atgacggtac tgatgccgcc
     8521 gcgggtccag ccggtgctgg cgtcgtcgtg gatgtagggc cacagttgcc gcgcgtagtc
     8581 ggggtcgggg ccgccggcgt gctcgtagac gatctcgttg ttcgcgtcgc cggtgccgat
     8641 cagcacgacc ggtgagcgcc agtcaccctc gccgacgcgc cggcccgaga acgtgtcacc
     8701 gaagaccgac accagtttcc cgttcggcgc caacaccgac gcgcccaggt cggtgcacgt
     8761 aacgccccac tgctccgtga tattcggacc cgtcacgtcg gccaacttgc ccgtcaacat
     8821 gcgatcaaag atgccacgac ttatacggtg aagcgatgag tcgacgagcg attgcagtcg
     8881 tcgcgctctg gttgtgcgcg tgttcggccg gtcaacgggt gaacctcggc gacgaggcgt
     8941 cgggaaacct cgtcgccgcg aaccgggcgt ggtggatgag cgtgtttcgt cgaacccgag
     9001 ctgttgatcc tcgacgaagc aactgcctcc cttgatgttt cggtgcaggc gcgtgttctc
     9061 gacctgtttc tggttctgca gcgcgatctt cgcctcacct acctgctcat cgcgcacgat
     9121 ctggcggtgg tccggcagat gagccacgac atcgtcgtga tgcggaaggg tgaaattgtg
     9181 gaatacggct ctgcgggtga ccttttcgcg gcgccagagc acgactacac acgcgccctt
     9241 ctcgcggcgg tgccgccgca gcgaccgcgg tgcccgtcgt gagttggtgg ctttgcggtg
     9301 ccgaacgaac gctgaccgaa gacgttgcgg cgccgcctgg tcgggttcgg gaattctatg
     9361 tcgacctgga caacatccgg ctcgtccatc cgctggtggt ctctgtgcag acgatctccc
     9421 gcactgacac gccgaacggc tatatgcaga cctgccgcgt acaggaccgg attccgttgg
     9481 ggttgatcac gttgcgcacc cggtatgtgg cacggctgca cgttcccgct tccggcgaag
     9541 tgatcgccga cgctcgccag tttccgtggg tgcggctgcg cagcgtcgtg acgttcggcg
     9601 aaacagacac cggcacacgg attgtcgagc acatgcgtat tcaagcgccg cggttgctag
     9661 cggcggtgac gatacgcgag gcggtggagg cgcacaccgt gatgctggca aacatcaggc
     9721 agcacttcgc tagataggca gcgcctgcat cggcatgtcg acagtgccct ggcaggcgcc
     9781 gctggagatg tcggtgtgca tttcaccggt taacgacccg tccggctggg gcgtgtagct
     9841 cactcgagaa ccggcggggc tccattcgat cgtgccgtca gggaacaggc agtcccaacg
     9901 ccatgtgctg gtcgacgtcc acgccgaccc gtcccaggtg aactgaaccg gttgcgggat
     9961 ggtcgggttc ctcggcggag gaccgtccgt gatcgtcgcc acgcaatgtc ccgacgagca
    10021 tgacgaagag aaggtgtaga tgtcgacgtg catggactcg ggctgattga ccgccatgct
    10081 ggtcccgctc ttctgatagg tgaagaacgt cacctcgtac tgcccgttcc aggatggatc
    10141 ggcgagcgcc ggtggagcta ggcacaccga tgtgcctagc gatgcgacag ccaacgcgac
    10201 aagggcgctg atgtgatgtc gggtcatttc ttctccccct actgcgttcg gagcccccgg
    10261 atcgcgttag tgtcccagac ggctgctctt gcgtctcaat tttcacaatt ccaacactgg
    10321 acgcacgggt gccaaggaac cgatgggctt tcgggactcg aagacgctgc tgagtgcggg
    10381 tactacgctg cggacatggc tgacgtggcg acacgactcg ccgggattgt cggggacaag
    10441 aatcttctga ccggcaacgc aatctccgag gactacgcac acgacgaagc gctgaccgag
    10501 gcgccgcaga aacccgcgta cgtcgcgaag cccgccacgg cggacgaggt tgcggagctg
    10561 ctgaagctgg cgacggagca gcggctgccg gtgactgcgc gggggtccgg gtgcggcttg
    10621 tcgggagcgg cgcagccggt agagggcggg ttgctgatct cgttcgagcg gatgaacgcc
    10681 gtgctcgaag tcgacgccga gaatcacgtc gccgtggtgc agccgggcgt gacgctgacc
    10741 caactcgacg ccaagaccgc cgaggtgggg ctcagctaca ccgtgtaccc gggcgagttg
    10801 tcctcgagcg tcggcgggaa cgtcgggaca aacgccggtg gaatgcgagc ggtcaaatac
    10861 ggtgtgaccc gccacaacgt gctcgggttg caggcggtgt tgccgacggg cgagatcatc
    10921 cgaaccggcg ggaagatgtc gaagatatcg agcggctacg acctcaccca gctgatcatc
    10981 ggctccgagg gcacgctggc actggccacc gaggtcatcg tcaagctggt tccgcgtctg
    11041 gcgcacggcg cgacggtgct cgcaccgttc gacgacttcg accaggtgat ggccgcggtc
    11101 ccgaagatcg tcgccagcgg gctcgctccg agcatcctgg agtacgtcga caacatcact
    11161 atggccgcgc tggtgcaccg ggagaacctg gagctgggag tgccagacga gatccgcgag
    11221 aagtgcgagg cgtatctcgt tgtcgcgctg gagaatcggg acgctgaccg gttgcacgag
    11281 gacgcggaga gccttggcga attgctcagc gagctaggtg cggtggacgc ctacgtgctc
    11341 gagggtggtg ccgcccgcag gctgatcgag gcgcgggaga aagtgttctg ggcggcgaag
    11401 gcgatgggcg tggacgacat catcgacgtc gtcgtgcccc gtgcgtcgat gccgcggttc
    11461 ctcaccacgg cgcgcgagct ggcggtcggc gccggcgctc acgccctggg ctgcggccat
    11521 gccggcgacg gcaacgtgca tgtcgggatt ttttgcccgg acgcagacat ccgtaagaag
    11581 ctgctcgccg agatcttcgc ggtcgcgatg gaactcggcg gtgcgatatc cggggagcac
    11641 ggtcttggcc gcgccaagac gagctacttc ctggaactcg aagacccggc caagatcgcg
    11701 cttctgcgcc ggatcaaaga gagcttcgac ccggcgggga ttctcaatcc gggggttttg
    11761 ttcgtcgacg cgtgacagtg tcgccgggtt tgtgtacagt cgcacatgtg ttcgaattaa
    11821 cggtgttgtc ggcggtggat ccgcatgccg acgaggcagc gttgaccgag cagatcgccg
    11881 agttggagcg ggtgaagtcc gccgcgcggc ggcgggtcag gcgcgcgcgg ccgcggcatt
    11941 ggacgaattg cgccgcgccg atgaggcggc ggccggggtt ccgacgcgga agcgcgggcg
    12001 gggcgtggcc agcgagatcg cgctggcgcg acgggattcg ccgacccggg gcgggcggca
    12061 tctcgggttc gccaaggcgc tggtctacga gatgccgtgc acgatggcgg ccctcgagtg
    12121 cggagcgctg tcggagtggc gggcctcgtt gatcgtgcgg gaatccgcgt gcctggacgt
    12181 cgaagaccgg caggtgctgg atgccgagtt gtgcgccgac gtctcgaagc tggacggcat
    12241 gggtgacgcg cgaattgctg cggcggccaa ggagatcgcc tatcggctgg acgcgcaggc
    12301 cgtcgtcgac cgcgccgcca aagcggaagc cgaccgcacc gtcaccattc ggccggcccc
    12361 cgacacgatg acgtatgtga ccgcgctcct gccggtggcg cagggcgtcg gtgtgtacgc
    12421 ggcgctgaag cgggccgccg acacgacttt cgacgaccgc tcccgcggcc agatcatggc
    12481 cgacacgctg gtggagcgcg tcaccgggcg gccggcggac caaccggaac cggttgcggt
    12541 gaacctggtg atttccgacg atgcgctgct gggcggcggc aacacccccg cggtggtaga
    12601 cggctacggc ccgattccgg cggcggtggc gcgggggctc gtgcagggcg cggtcaccga
    12661 tgagcgctcg aaggcgacgt tgcgccggct gtaccgccgc ccgaactcga ggaacttggt
    12721 ggcgatggaa tcacgctcgc ggtgctttcc acgcggattg gcacgattca tcggcttgcg
    12781 cgaccagacg tgccgcaccc cgtactgcga cgcgccgatc cggcaccgcg accatgccca
    12841 accgcggaac cgcggcgggc cgaccagttg ggtgaacgga ctcggtatgt gcgaacggtg
    12901 caactatgaa aaggaagcac cgggctggcg ggtgaccggc ggtgatgaaa acggcacgca
    12961 cacagctgaa ttcgtcacgc ccactggcgc gcgctaccgt tccaccgctc cgccgctacc
    13021 tggtgcaccc ccgataacgg ttgtcgaagt caaagccaga atcggcatcg agatgccaaa
    13081 gctacatgcc gcgtgagacg gcctacgcaa gaacgcgaaa catatggtgg ccggtggctt
    13141 agtggtctcg ctcgtaagtt cgtcgggggg tcatgcccag gcggttgggt cgcccaggta
    13201 gaggccgcag gc
//
LOCUS       DASWBF010000250         9363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_418043,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000250 DASWBF010000000
VERSION     DASWBF010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418043"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1656)
                     /locus_tag="VF874_09075"
     CDS             complement(67..1656)
                     /locus_tag="VF874_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9176223.1"
                     /translation="MLRSTLDTRAPAYEESVAAMTAKLTDIDAEFEKALGGGGPKYVE
                     RHHARGKLTPRERIELLIDPDSPFLELSPLAAYGTDFAVGASAVVGIGVVSGVECLLN
                     ANDPTVKGGTSNPWTLRKILRANQIAFENRLPVISLVESGGADLPTQKEIFIPGGQMF
                     RDLTRLSAAGIPTIALVFGNSTAGGAYIPGMSDHVVMIKERSKVFLAGPPLVKMATGE
                     ESDDESLGGAEMHARTSGLADYFAVDELDAIRIGRRIVARLNWRKRGPAPGPVVEPVA
                     DPEELLGVVPPDLRTPFDPREVIARFTDGSEFDEFKGLYGSSLVTGWARVHGYPIGIL
                     ANARGVLFSEESQKATQFIQLANRADTPLLFLHNTTGYMVGKQYEEGGMIKHGSMMIN
                     AVSNSTVPHISLLIGASYGAGHYGMCGRAYDPRFLFAWPSAKSAVMGGAQLAGVLSIV
                     SRAAAEARGQQVNEEADAALKAAVEAQIEAESLPMVLSGMLYDDGIIDPRDTRTVLGM
                     CLSAIANAPVEGTTNFGVFRM"
     gene            complement(1659..2834)
                     /locus_tag="VF874_09080"
     CDS             complement(1659..2834)
                     /locus_tag="VF874_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176224.1"
                     /translation="MSMWTTPERDELRKTVRAFVEREVLAHVDEWERIGELPRDLHRR
                     AGEAGLLGAGFPEAVGGGGGDGADSLIICEEMHYAGAPGGVFASLFTCGIAVPHMIAS
                     GDERLIERFVRPTLRGELIGALAITEPDGGSDVGHLRTSARRDGDDYIVNGAKTYITS
                     GVRADFVVTAVRTGGPGAAGVSLLVVEKGTPGFEVSRKLDKMGWRSSDTAELSYTDVR
                     VPVANLVAAENTGFLQIAAAFVSERVGLAAQAYASAQRCLDITVEWCRNRETFGRPLI
                     SRQSVQNTLAEMARRIDVARVYTRNVVERQLAAGAAGPTSGTETNLIPQVCFAKNTAV
                     EAGEWVASQAVQLFGGLGYMAESEVERQYRDMRILGIGGGTTEILTSLAAKTLGYQS"
     gene            complement(2831..4537)
                     /locus_tag="VF874_09085"
     CDS             complement(2831..4537)
                     /locus_tag="VF874_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX9176225.1"
                     /translation="MAVAQDVVRIGNCSGFYGDRLSAMREMLTGGELDYLTGDYLAEL
                     TMLILARDKAKSPERGYAKTFLTQLEECLGVAHDNGVRIVANAGGLNPAGLADAVRAL
                     ASRLGVPVNVAHVEGDDLLPRAAELGFGSPLAANAYLGAWGIVDCLNAGADVVVTGRV
                     TDASVVVGPAAAHFGWSRTDYGRLAGAVAAGHVIECGAQATGGNYAFFTDIDDLTHPG
                     FPLAEVRSDGSSVITKHPGTGGAVTVGTVTAQLLYEITGARYANPDVTLRVDTIELAD
                     DGADRVLISGVRGEPPPPTLKVSLNTIGGFRNAMTFVLTGLDIDAKAELVRRQLETAL
                     TVKPAELEWTLARTDHPDADTEEAASTLLRCVARDPDPANVGRQFSSAAVELALASYP
                     GFTTTAPPGDGQVYGVFTPGSVRADQVPHVAVHADGHRVDIPSATETVELAPVPPFAL
                     PVPLPFGETRRVPLGVIAGARSGDKGGSANVGVWVRTEDHWRWLASTLSVDKLRELLP
                     EVADLPVTRHVLPNLFAVNFVVEGILGEGVAYQARFDPQAKGLGEWLRSRYVDIPEAL
                     LA"
     gene            4739..4912
                     /gene="rpmF"
                     /locus_tag="VF874_09090"
     CDS             4739..4912
                     /gene="rpmF"
                     /locus_tag="VF874_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX9176226.1"
                     /translation="MAVPKRRMSRANTRSRRAQWKAKSPELVNVTVAGQQHKVPRRLL
                     KAARLGLIDLDHR"
     gene            4983..5669
                     /locus_tag="VF874_09095"
     CDS             4983..5669
                     /locus_tag="VF874_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9176227.1"
                     /translation="MRILVVDDDRAVRESLRRSLSFNGYSVSLAQDGVEALYAIANER
                     PDAVVLDVMMPRLDGLEVCRQLRSTGDDLPILVLTARDSVSERVAGLDAGADDYLPKP
                     FALEELLARMRALLRRTVPDDSANSVPMTFSDLSLDPVTREVTRGERRISLTRTEFAL
                     LEMLIANPRRVLTRSRILEEVWGFDFPTSGNALEVYVGYLRRKTEAEGEPRLIHTVRG
                     VGYVLRETPP"
     gene            5669..7156
                     /locus_tag="VF874_09100"
     CDS             5669..7156
                     /locus_tag="VF874_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9176228.1"
                     /translation="MSIFQSPRHTALPASSSLSLRWRVMLLAMSMVAMVVVLIAVAAY
                     VVVSAALYRNVDNQLQTRSQLLIESGALDADPGKAIGGTAYSDVNAMLVIPGRAIYTA
                     NQAGQQLPFGQQEKDVVDGELLLSLRAAGDQRVLATHLPNGSTLLVSKSLKPTNSVLR
                     QLGWVLLVVGGVGLVIAAVAGGMVASAGLRPVRRLTDAAERVARTDDLRPIPVTGSDE
                     LARLTENFNMMLRALAESRERQARLVTDAGHELRTPLTSLRTNVELLIASMVPGAPRL
                     PETEMAGLRADVLAQIEELSTLVGDLVDLTRDDAGEVVHETVDLSEFVDRSLERVRRR
                     RNDIQFEAQTTPWQVFGDAAGLSRAVLNLLDNAAKWSPPGGRVSIRLAQVDPSHAELV
                     VADHGPGIPPRERQLVFERFYRSASARALPGSGLGLAIVKHVVMKHGGVLRIEDTVQG
                     GQPPGTSIHVVLPGRPMPAAPAADVKKSVDDSREISVDSQSSVAR"
     gene            7206..8465
                     /locus_tag="VF874_09105"
     CDS             7206..8465
                     /locus_tag="VF874_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9176229.1"
                     /translation="MTNHPRYSAPQQPYPPHTQRYSPYDWRATQQIPIPRQPHTPHPV
                     PQPPPVPSAPQKRSHAGGFIAGAMAIAVVSAGVGGGVALAVRPDHPNAATTVATAPGA
                     APGAPAASLPVGSVEQVAAKVVPSVVKLETSMGQQSEEGSGIILTSDGLILTNNHVVA
                     SATQNGPTTTVTFSDGRTVPFTVVGTDPSTDIAVVRAEGVSGLTPVTIGSSSDLKVGQ
                     DVVAVGSPLGLEGTVTTGIISALNRPVATGGDAGDQNTVLDAIQTDAAINPGNSGGAL
                     VNMNGELVGVNSAIATLGADATGPASGSIGLGFAIPVDQAKRIADELIQTGTATHSSL
                     GVQVRNDATAHGAKIMEVTPGGAADAAGLPSGVVVTKLDDRVINGADALVAAVRSKAP
                     GDKVTLTYTAPGGDQTVEVTLGTAQQR"
     gene            8495..9040
                     /locus_tag="VF874_09110"
     CDS             8495..9040
                     /locus_tag="VF874_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX9176230.1"
                     /translation="MRLVTPPSAPRYTVASMEQPVELVGRALVVVVDDRTAHGDEQDH
                     SGPLVTELLTEAGFVVDGVVAVAADEVEIRNALNTAVIGGVDLVVSVGGTGVTPRDVT
                     PEATRDILDRELLGISEALRASGLSAGITDAGLSRGLAGISGSTLVVNIAGSRYAVRD
                     GMATLNPLATQIIGQLSSLEI"
BASE COUNT         1525 a         3196 c         3115 g         1527 t
ORIGIN      
        1 acgtcgcgaa aacgcgccgg gcgatctcac cgcggttggc cacgaggact cgactgacca
       61 tgggcctcac atccggaaga cgccgaaatt ggtggtcccc tcgaccggcg cattggcgat
      121 ggcggacagg cacattccga gcacggtgcg ggtgtcgcga gggtcgatga tgccgtcgtc
      181 gtacagcatg ccggacagca ccatcggcag cgactcggcc tcgatctgcg cctcgacggc
      241 ggccttcagc gcagcgtcgg cctcctcgtt gacctgctgc ccgcgcgcct cggcggcggc
      301 tcggctgacg atcgacagca ctccggccag ctgcgcgccg cccatcacgg cggatttggc
      361 gctgggccag gcgaacagga agcgcgggtc gtaggcgcgc ccgcacatgc cgtagtggcc
      421 agcgccgtag gaggcgccga ttagcagcga gatatgtggc accgtcgagt tcgacaccgc
      481 gttgatcatc atcgagccgt gcttgatcat gcctccctcc tcgtactgct tgcccaccat
      541 gtagccggtc gtgttgtgca gaaacagaag tggcgtgtcg gcgcggttgg ccagctggat
      601 gaactgggtg gccttctgcg attcctcgct gaacagcacc ccgcgggcgt tggccaggat
      661 gccgatcggg tagccgtgca cccgcgccca gccggtcacc agcgacgagc cgtagagccc
      721 tttgaactcg tcgaactccg agccgtcggt gaagcgggcg atcacctcgc gcgggtcgaa
      781 cggggtccgc aggtcgggtg gcaccacgcc gagcagctcc tcggggtcgg ccaccggttc
      841 cacgacgggt cccggtgccg ggccccgctt gcgccagttc agccgcgcca cgatccgtcg
      901 gccgatccgg atcgcgtcga gctcgtcgac cgcgaagtag tcggccaaac cggacgtgcg
      961 ggcgtgcatc tcggcaccgc cgagcgactc gtcgtcggat tcctcgccgg tggccatctt
     1021 caccagcggc gggccggcca ggaacacctt cgagcgttcc ttgatcatga ccacgtggtc
     1081 ggacatcccg gggatgtacg cgccgcccgc agtcgagttg ccgaatacca gtgcgatggt
     1141 ggggatcccg gcggcggaga gccgggtcag gtcgcggaac atctgaccgc cggggatgaa
     1201 gatctccttc tgggtcggca ggtcggcgcc gccggattcc accaatgaga tgactggcag
     1261 ccggttctcg aacgcgatct ggttggcccg cagtattttt cgtaacgtcc agggattgct
     1321 agtaccgccc ttgacggtcg ggtcgttggc gttcagcagg cactcgacgc cgctcaccac
     1381 gccgatgccc acaaccgccg aggcgccgac cgcgaagtcg gtgccgtacg ccgccaacgg
     1441 gctcaactcc aggaacggcg aatcggggtc gatcagcagc tcgatacgct cacggggtgt
     1501 caatttgcct cgggcgtggt gccgttcgac gtatttcggg ccgccaccgc ccagtgcctt
     1561 ctcaaactcg gcgtcgatgt cggtgagctt ggccgtcatc gcggccaccg actcttcgta
     1621 cgccggtgcc cgagtgtcca gcgtggaccg cagcacgctc acgactggta tcccaacgtc
     1681 ttcgcggcga gcgaggtgag gatctccgtt gtgcccccgc cgattccgag aatccgcatg
     1741 tcacggtact gccgctcgac ctcactctcg gccatgtagc ccaacccgcc gaacagctgc
     1801 acggcctgac tcgcgaccca ctcgccggct tcgacggcgg tgttcttggc gaagcacacc
     1861 tgcgggatca aattcgtctc tgtgcctgat gtcggccctg cggctccggc ggccaactgc
     1921 cgctccacca cgttgcgggt gtagacgcgc gcaacgtcga tccgtcgcgc catctccgcg
     1981 agcgtgttct gcaccgactg acgcgaaatc agcggccgcc caaaggtttc ccggttgcgg
     2041 caccattcga cggtgatgtc caggcagcgc tgcgcgctcg catacgcttg cgcggccaga
     2101 ccgacccgtt cggagacgaa cgccgccgcg atctgcagga agccggtgtt ctcggcggcg
     2161 acgaggttcg cgaccggaac gcgcacgtcc gtgtaggaca actcggcggt gtccgaggac
     2221 cgccagccca tcttgtccag cttgcggctg acctcgaaac ccggtgtgcc cttttcgacg
     2281 acgagcagcg aaacccccgc ggcacccggc ccgccggtgc gcacggccgt caccacgaag
     2341 tcggcacgca cacccgaggt gatgtaggtc ttcgcgccgt tgacgatgta gtcgtcgccg
     2401 tcacggcggg cgctggtgcg caggtgcccg acgtccgagc cgccgtcggg ctcggtgatc
     2461 gccagcgcgc cgatcagctc gccgcgcagc gtcggccgca cgaagcgttc gatcaggcgc
     2521 tcgtcgccgg aggcgatcat gtgcggcacc gcgatcccgc acgtgaacag cgacgcgaac
     2581 accccgcccg gcgcgccggc gtagtgcatc tcctcgcaga tgatcaggga gtcggcgccg
     2641 tcgccaccgc cgccgccgac ggcttcgggg aatcccgcac ccaagagccc ggcctcgccg
     2701 gctcgacggt gcagatcgcg cggcagctcg ccgatccgct cccactcgtc gacatgggcc
     2761 agtacctcgc gttctacgaa cgcccgcacg gtctttcgca gttcgtcacg ctcgggcgtg
     2821 gtccacatac tcacgcgagc aatgcctccg ggatgtcgac gtatcggctc cgcagccact
     2881 cgcccagtcc tttggcctgc gggtcgaagc gcgcctgata ggccacaccc tcgccgagga
     2941 tgccctcgac cacgaagttg accgcgaaga ggttcggcag cacgtggcgg gtgaccggca
     3001 ggtcggccac ctcgggcagc agttcgcgca gcttgtccac cgacagcgtg ctggccagcc
     3061 accgccagtg gtcctcggtg cgcacccaca cgccgacgtt ggcgctgcca cccttgtcgc
     3121 cgctgcgcgc acccgcgatc acgccgagtg gcacgcgtcg cgtctcgccg aacggaagcg
     3181 gcaccggcag cgcgaacggc ggaacgggtg ccaactccac agtctcagtg gcagaaggga
     3241 tgtcaacccg gtgaccgtcg gcgtgcaccg cgacgtgcgg cacctggtcg gcgcgcactg
     3301 aaccgggcgt gaacaccccg tagacctggc cgtcgcccgg cggagcggtg gtggtgaagc
     3361 cggggtagct cgccagcgct aattcgactg cggctgagga gaactggcgt cccacattcg
     3421 ccgggtccgg gtcgcgggcc acgcagcgca gcagcgtgct cgccgcctcc tcggtgtcgg
     3481 cgtcggggtg gtcggtgcgc gccagcgtcc actccaactc cgcgggcttg acggtcagcg
     3541 ccgtctccag ctgccggcgc accagctcgg ccttcgcgtc gatgtccagg ccggtcagca
     3601 cgaacgtcat cgcgttgcgg aacccgccga tggtgttcag cgacaccttc agcgtcggcg
     3661 gcggcggctc accgcgcacg ccgctgatca gtacccggtc ggcgccgtcg tcggctagct
     3721 cgatcgtgtc gacgcgcagt gtcacgtcgg ggttggcgta gcgcgctccg gtgatctcgt
     3781 agagaagctg ggcggtcacg gtcccgacgg tgaccgcgcc gccggtgccc gggtgcttgg
     3841 tgatcaccga cgagccgtcc gaccgcacct ccgcgagcgg gaaccccggg tgcgtcaggt
     3901 cgtcgatgtc ggtgaagaac gcgtagttgc cgccggtggc ctgcgcgccg cattcgatga
     3961 cgtggcccgc ggcgacggcg ccggccagcc tgccgtagtc ggtgcgcgac cagccgaagt
     4021 gcgcggccgc aggcccgacg accaccgatg cgtccgtgac ccggccggtg acgacgacgt
     4081 ccgcgccggc attcaggcag tcgacgatgc cccacgcccc gaggtaggcg ttcgcggcca
     4141 gcggcgagcc gaagcccaat tcggcggccc gcggcagcag gtcgtcgccc tcgacgtgag
     4201 cgacgttcac cggcacgccg aggcgcgagg ccagcgcccg caccgcgtcg gcaagcccgg
     4261 ccgggttcag tccgccggcg ttcgcgacga tccgcacccc gttgtcgtgc gccactccca
     4321 ggcactcctc gagctgggtc agaaacgtct tcgcgtagcc gcgctcgggt gacttcgcct
     4381 tatcgcgcgc gaggatcagc atcgtgagct cggccagata gtcgccggtc aggtagtcca
     4441 gctcaccgcc ggtcagcatc tcgcgcatcg ccgagaggcg gtcgccgtag aagcccgagc
     4501 agttgccgat gcggacgaca tcttgcgcga cggccaccac atcccctccc gccggacttc
     4561 tcgaccaacc ggtaggttac ccccgcgatt cttcgccgag atagcggtca gggctgtgat
     4621 cggcgctcga ccacgaccat gagtgctatc tcggcgcaca gcggggccgc gctttggaga
     4681 cgtcgccggt caccggctat cctgaaccca atccccaact agatggagaa gttcgatcat
     4741 ggctgtgccg aagcgcagga tgtcgcgtgc gaacacccgg agccgtcgcg cgcagtggaa
     4801 ggccaagagc cctgagctcg tcaacgtcac ggtcgccggc cagcagcaca aggtgccgcg
     4861 gcggctgctc aaggccgcgc gtcttggcct gatcgacctc gaccaccgct aacccgcaag
     4921 cctgcgcgtt ttccggcgcg tctctcagcc cactctcagg cgttccgtag agactagggg
     4981 ccgtgcggat acttgtcgtt gacgacgatc gcgccgtgcg cgagtccctg cgccgatccc
     5041 tttctttcaa cggttactcc gtatcgctgg cgcaggacgg cgtggaggcg ctttacgcaa
     5101 tagccaacga gcgccccgac gccgtggtgc tcgacgtgat gatgccgcgg ctggacgggc
     5161 tggaggtgtg ccgacagctc cgcagcaccg gcgatgacct gccgattctg gtgctgacag
     5221 cgcgcgattc ggtgtccgag cgggtcgccg ggctcgacgc cggtgccgac gactacctgc
     5281 cgaagccgtt cgcgctcgag gagctgctcg cccggatgcg cgcgctgctg cgtcgcacgg
     5341 tgcccgacga cagcgcgaac tccgtgccga tgacgttctc ggacctgagc ctcgacccgg
     5401 tgacccgcga ggtgacgcgc ggcgagcgtc gcatcagcct cacccgcacc gagttcgcgc
     5461 tgctggaaat gctcatcgcc aacccgcgtc gagtgctgac ccgcagtcgc attctcgagg
     5521 aggtctgggg attcgacttc ccgacctccg gcaatgcgct cgaggtgtac gtcggctacc
     5581 tgcgccgcaa gaccgaagcc gaaggagaac cccggctgat ccacaccgtg cgcggggtcg
     5641 gatatgtgct gcgtgaaacc ccaccctgat gtcgatcttc cagtcgccac ggcacaccgc
     5701 acttcccgcc agcagctcgt tgtcgctgcg ctggcgggtg atgttgcttg ccatgtcgat
     5761 ggtggcgatg gtggttgtgc tgatcgcggt ggctgcctac gtcgtcgtgt cggcggcgct
     5821 gtaccgcaac gtggacaacc aactgcagac acgttcgcaa ctgctcatcg aaagcggcgc
     5881 gctcgacgcc gatccgggca aggcgatcgg aggcactgcg tactcagacg tcaacgcgat
     5941 gctggtgatt cccggccggg cgatctacac cgcgaatcaa gcaggtcagc agctgccgtt
     6001 cggccagcag gaaaaggacg tggtcgacgg cgaactgctg ctgtcgttgc gggccgccgg
     6061 cgatcagcgg gtgctggcca ctcacttgcc taacggcagt acgctgttgg tctccaagag
     6121 cctgaagccg acgaattcgg ttttgcgcca actgggttgg gtgctgctcg tcgtcggtgg
     6181 cgtcggcctg gtgatcgcgg cggtggccgg cgggatggtg gcgagcgcag gactacggcc
     6241 ggttcgcagg ctcaccgacg ccgccgagcg agtggcgaga accgacgacc tgcggcccat
     6301 tcccgtgacg ggtagtgacg agttggcaag gctcaccgag aatttcaaca tgatgctgcg
     6361 ggcgctcgcg gaatcacgag agcgccaagc gcgattggtc accgacgccg ggcacgagtt
     6421 gcgaacgccg ctgacctcct tgcggacgaa cgtcgagctg ttgatcgcct cgatggtgcc
     6481 cggtgcgccg cggctgcccg agaccgagat ggccggtctg cgcgcagacg tgctggcgca
     6541 gatcgaagaa ttgtccactc tggtcggaga tctggtagac ctcactcgcg acgacgccgg
     6601 cgaggtagta cacgagaccg tcgacctgtc cgaatttgtg gaccgcagtc tcgagcgagt
     6661 tcgtcggcgc cgcaacgata ttcagtttga agcgcagacc accccgtggc aggtgttcgg
     6721 cgatgcggcg ggcttgtcgc gggcggtgct taacctgctc gacaacgccg caaagtggag
     6781 tccgcccggt ggccgggtga gtatccgcct ggcgcaagtt gatccgtcac atgcagaact
     6841 ggtggtcgcc gaccacgggc caggcattcc acccagagaa cgccagctgg tgttcgagcg
     6901 gttctaccgc tcggcatcag cgcgcgcgct gcccggctcg ggattggggt tggccatcgt
     6961 caagcacgtc gtgatgaaac acggtggcgt tcttcggatt gaagacacgg tccaaggcgg
     7021 tcagccgccc ggtacctcaa ttcatgtcgt gctgccggga cggccgatgc ctgccgcacc
     7081 cgccgccgat gttaaaaagt ctgtggatga ttcgagggag atctcagtgg attctcagtc
     7141 gagtgttgca cgctagaggc aacgttctca gcactgacca ctcgagaaag agcgaatcag
     7201 cgacaatgac caaccatccc aggtattcgg cgccgcagca gccgtacccc ccgcacacgc
     7261 agcggtattc gccgtacgac tggcgcgcca ctcagcagat tccgatcccc cgtcagcccc
     7321 acaccccaca tcccgtccca cagccgccgc ccgtcccgtc ggctccgcaa aagcgttccc
     7381 acgcaggcgg tttcattgcc ggtgcgatgg ctatcgccgt cgtctccgcc ggtgttggcg
     7441 gcggagtcgc gttggcggtc aggccggatc acccgaacgc cgcgaccacc gtcgcgacgg
     7501 ccccgggcgc tgcgccgggg gcgccggcgg ccagcctgcc cgtcggatcc gttgagcagg
     7561 tggcggccaa ggtagtgccg agcgtcgtga agctcgaaac ctcgatggga cagcagtcgg
     7621 aagaaggctc cggcatcatc ctgacctcgg acggcttaat tctcaccaac aatcacgtgg
     7681 tggcctcggc gacacagaac ggcccgacga ccacggtcac attcagcgat ggccgcaccg
     7741 tgccgttcac agtggtgggc accgatccgt ccaccgacat cgcggtcgtg cgcgccgagg
     7801 gagtctccgg cctgacgccg gtcacgatcg gctcgtcgtc cgacctcaag gtcggtcagg
     7861 acgtggtggc ggtcgggtcg cctctgggtc tcgaaggcac cgtcacgacc ggcatcatca
     7921 gcgcgttgaa ccgtccggtg gccaccggcg gcgacgcagg cgaccagaac accgtgctcg
     7981 acgcgatcca gaccgacgcg gcgatcaacc ccggcaactc cggtggcgcg ctggtgaaca
     8041 tgaacggcga gttggtcggc gtcaactccg cgatcgcaac cctcggagcc gatgccaccg
     8101 gcccggcgag cggatcgatc ggcttgggct tcgcgatccc cgtcgatcag gccaagcgca
     8161 tcgccgacga actgatccaa accggcacgg cgacgcactc gtcgctcggc gttcaggtgc
     8221 gcaatgacgc gaccgcccac ggcgccaaga tcatggaagt gacgcccggg ggtgccgccg
     8281 acgcggccgg cctgcccagt ggcgtggtcg tgaccaagct cgacgaccgc gtgatcaacg
     8341 gcgcggacgc gctggtcgcc gccgtccggt cgaaggcgcc gggggacaag gtgacgttga
     8401 cctacaccgc gccgggcggc gaccagacgg tcgaggtgac gctcgggacg gcgcagcagc
     8461 gatgagaggc gtgcgcgaaa ggcggcacac gacgatgaga ctggtgacgc caccgtcggc
     8521 gcccagatat acggtggcat ccatggaaca acccgtggag ttggtgggcc gcgcactggt
     8581 tgtcgtcgtc gacgaccgca ccgctcacgg cgacgagcag gaccatagcg ggccgctggt
     8641 gacggagctt ctcaccgagg ccggcttcgt cgtcgacggt gtcgtagcgg tggcagccga
     8701 cgaggtcgaa attcgtaacg ccctgaacac cgcggtgatc ggcggcgtcg accttgtggt
     8761 gtccgtgggc ggtaccggag tcacaccgcg cgacgtcact ccggaggcca cccgcgacat
     8821 cctcgaccgc gaactcctgg gcatctccga agccttacgc gcatcgggtc tgtcggcggg
     8881 catcaccgat gccgggctgt cgcgtgggct ggcgggcatc tccggcagca cgctcgtcgt
     8941 caacatcgcc ggctccagat atgcggtgcg agacggcatg gcgacgctga acccgctcgc
     9001 cacgcaaatc atcggccaac tctccagcct cgagatctaa aacgagcgtg cgtcgctagt
     9061 acggccacga aacgcacagg ttcaagggct gtgaacgtcc tgttaactgc ggcttcccaa
     9121 gaagatgtga tgttcgtcac aatctggacg gattatgacg aagccatctc ttagcccttc
     9181 ttacttggca aatacggaca gtggcccggc taactgtttg gcaaccacgg tccgaattcc
     9241 cggcgcgcct cacagccggt ttccgcgacg gctaagtcac tgcggctcaa cgctcttttt
     9301 tgggtgcctc ggtccggcct cgggatgagt cgttcggcgt tgggtgcaca gggttgatga
     9361 gcc
//
LOCUS       DASWBF010000251         6151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_23399, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000251 DASWBF010000000
VERSION     DASWBF010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23399"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VF874_09115"
     CDS             <1..318
                     /locus_tag="VF874_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HEX9176231.1"
                     /translation="LALREAAAAAKINLLEPVDTVSVVVPDDLVGAVMSDLSGRRGRL
                     LGTDNVAGNHTVVRAEVPEVELTRYAIDLRSLSHGAGSFTRTFTRYEPMPESAAAKVK
                     ASA"
     gene            complement(315..722)
                     /locus_tag="VF874_09120"
     CDS             complement(315..722)
                     /locus_tag="VF874_09120"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEX9176232.1"
                     /translation="MLTNRIINWTAAAALAAGTLASAVVVTAGPAQAAQKDVFAAIAY
                     SESEGYAGWVTNAQSQDAAQQGAVEQCEAESNSACELAAWVKNGCVAIALDKHDKWSG
                     GYGPDSAAAEASAVEDLPGGQVKKVVCTPNVES"
     gene            839..1945
                     /locus_tag="VF874_09125"
     CDS             839..1945
                     /locus_tag="VF874_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX9176233.1"
                     /translation="MTAITPFRIDVSDEDLDDLRQRLARTRWPERECVDDWSQGIPLD
                     YTRELASYWADGYDWRAREAALNRFDQFTTEIDGLDIHFIHQRSSHDDAFPLIITHGW
                     PGSIVEFHKVIEPLTETFHVVCPSLPGYGFSGKPTTTGWGVDKVAQAWETLMTRLGYD
                     RYGAQGGDWGAAVTTAIGRNGESNHCAAIHLNFPIAFPPAGMTDPTPEEQDVLKSLDY
                     YQKWDSGYSKQQSTRPQTLGYGLVDSPVGQLAWIVEKFWSWMDCNGHPENVLTKDELL
                     DNVMMYWATASAASSARMYWESFRFFGRLDRVELPTGIAVFPKENIRAPRHWCEPTYN
                     ITRWTAMPRGGHFAAFEQPELFVEDVRAFFATVR"
     gene            complement(1947..2819)
                     /locus_tag="VF874_09130"
     CDS             complement(1947..2819)
                     /locus_tag="VF874_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9176234.1"
                     /translation="MTDSGKQRDQTDVEQFAEALLAAVEEQLNLPDVSPSHAMATAAM
                     ALLQLGIEFVRRYNRLDVRVESSALNGPVLFVANHGFGGIFDLNVFATYAALEQLELD
                     RPVTALTHQLAWTLGVGPFIEPVGARPASHDSAREAFGEGQHVLVFPGGDLDAAKTWH
                     ERNQVVFGGRSGFARLAIEFGVPIVPIVTAGASESLLVISRGERLARALRVDKLLRIK
                     ALPLTVSLPWGVNVGAVGLLPYLPLPTKLDTRVLTAIHAEPDEEPDAYAERVQSAMQS
                     ALDEMTANRKPLLG"
     gene            2983..4023
                     /locus_tag="VF874_09135"
     CDS             2983..4023
                     /locus_tag="VF874_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9176235.1"
                     /translation="MRSCRGRIDTHQHLIPPGYRNALRKAGIDEAGGRALPDWSPDAA
                     LQAMAALELATAIVSVSAPGTTFLPVAADAAALARDLNDYSAALVASQPDRFGFFATV
                     PMPHIDESVAEAVRALDGLDADGVVLLANSAGVYLGQDGQDELWAALDERSAVVFVHP
                     AELPGPAVPGVAPFAADFLLDTTRAAYLLVRNGIRRRYPNIRFILSHAGGFVPYASHR
                     MALAIMGDTGRSPADSLDDFADFYFDTALSSGSAALPTLLAFAKPGHVTFGSDWPFAP
                     VPAAKLFAAGLETYSGMDAGTHAAIDRTNALALFPRLGTAPAPTPASRIDAVRHIASR
                     VVMRGVARLISR"
     gene            4058..4972
                     /locus_tag="VF874_09140"
     CDS             4058..4972
                     /locus_tag="VF874_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9176236.1"
                     /translation="MPTALEVVDGVDLNGKTCVITGASSGLGRESARALASAGAQVVL
                     AARNPDALAESQSWVRAEVPDAATTTVHLDLTSLASIRAAAASIQEIAPAIHVLMNNA
                     GVMFTPFGRTTDGFEAQFGTNHLDHFELTRLLVPQLLAAGGARVVILSSDGHLISDVD
                     YDDPNWERRDYDKFLAYGASKTANILHMVELDRRLRDGAVRAYSVHPGIVATALARYM
                     TRDDFAKLNNFGSTSESENPVDIRRDFLTPDQGAATQVWAAVSPELAGLGALYLADCR
                     VRENVAPYAIDEQRSARLWALSESLCGN"
     gene            4986..>6151
                     /locus_tag="VF874_09145"
     CDS             4986..>6151
                     /locus_tag="VF874_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9176237.1"
                     /translation="MRWVTYRSDEGDRVGVVPDQSIYAVDAGITLIDLVARGHDGLRA
                     AGEDALRSPSAVLRLDEVSLRAPIPTPPAVRDSLCFLDHMRNCQQALGAGRVLKDTWY
                     RIPAFYFACPSTVFGPHDDVPTAPGSAWQDFELEIAAVIGTGGKDLSVEDAEQAIIGY
                     TILNDWSARDLQTLESQLGIGQAKGKDSGITLGPYLVTPDELAAYRRDGRLCLAVTAL
                     VNDTEIGSGTTADMDWSFAEVISYASRGVTLNPGDVVGSGTVPTCTLVEHLDLTAPDA
                     FVGWLHDGDTVTLRVQGLGETRQTVRATPGPRPLRPRRNPDTIARPARVNRAPTRIPY
                     VRGLHNVGERVWAWTLPDGGYGWSNAGLTAGEGASLLVDTLFDLHLTREMLAAM"
BASE COUNT         1019 a         2051 c         2011 g         1070 t
ORIGIN      
        1 ctggcgctgc gcgaggcggc ggctgcagcc aagatcaacc tcctcgagcc ggtggacact
       61 gtgtcggtgg tggttcccga cgatctcgtg ggcgcggtga tgagcgatct gtcgggtcgg
      121 cgcggccggc tgctgggcac cgacaacgtc gccggcaacc acacggtggt cagggccgag
      181 gtgccggagg tggagctgac gcgatacgcg atcgacctgc gctcgttgtc acacggcgcg
      241 ggttcgttca cccgtacctt cacccgctac gagccgatgc cggaatcggc ggcggcgaaa
      301 gtcaaagcga gtgcttaaga ctcgacgttg ggggtgcaga ccaccttctt gacctgcccg
      361 ccgggcaggt cttccaccgc actcgcctcg gcggcggcac tgtccggccc gtagccgccg
      421 gaccacttgt cgtgcttgtc gagagcgatc gcgacgcaac cgttcttgac ccacgcggcc
      481 aactcgcacg cgctattgga ctctgcttcg cactgttcga cggcgccttg ctgggctgcg
      541 tcttgggact gcgcgttggt cacccagccg gcgtatccct ccgactcgga gtaggcgatt
      601 gcagcgaaca cgtctttctg cgcggcctgt gcaggcccgg cggtgacgac gacggcgctg
      661 gccagcgtgc cggcggccaa cgcggcggcg gcggtccagt tgatgatccg gttggtcaac
      721 atagttcgac ttcctttgcg gtcgttgggt gtcgagtgac ctcaccgtgc aacggacgac
      781 ttgaaggatt ctcaacagat tcttggcggg tcgcgtccac tcggcgcaag cttgagccat
      841 gaccgcgatc accccgttcc gcatcgacgt ctctgatgaa gatctcgacg acctgcgcca
      901 gcggctggcg cgcacccgat ggccggagcg tgagtgcgtc gacgactgga gccagggcat
      961 tccactggac tacacccgag aactcgccag ctattgggcc gacggctacg actggcgggc
     1021 ccgcgaagcg gcgctgaacc ggttcgacca gttcaccacc gagatcgacg gactggacat
     1081 ccatttcatt catcagcgct cgtcgcacga cgatgcgttc ccgttgatca tcacccatgg
     1141 ttggccgggt tcaattgtgg agtttcacaa ggtgatcgag ccgctcaccg agacatttca
     1201 cgtggtgtgc ccgtcgctgc ccgggtacgg attctccggc aagccgacga ctaccggctg
     1261 gggcgtcgac aaggtcgcgc aggcgtggga gacgctgatg acgcgcttgg gatacgaccg
     1321 ctacggcgcg cagggtggcg actggggcgc ggcggtcacg acagcgatcg gtcgcaacgg
     1381 cgaaagcaac cattgcgcgg cgatccacct gaactttccg attgcgtttc cccccgccgg
     1441 tatgaccgat ccgacgccgg aggaacagga cgtgctgaag tcgctcgatt actaccagaa
     1501 gtgggattcg ggctacagca agcagcagtc gacccggccg cagacacttg gctacggcct
     1561 ggtggattcg ccggtcgggc aactggcgtg gatcgtcgag aagttctggt cgtggatgga
     1621 ctgcaacggt catccggaga acgtgctgac gaaggacgaa ctgctcgaca acgtgatgat
     1681 gtactgggcc acggcgtcag cagcgtcgtc ggcgcggatg tactgggaaa gcttcagatt
     1741 cttcgggcgt ctcgaccgcg tcgaactgcc caccggcatc gctgtgtttc ccaaggagaa
     1801 tattcgcgcg ccgcgtcact ggtgcgagcc gacctacaac atcacccgct ggaccgccat
     1861 gccgcgcggc ggtcacttcg ccgcgttcga gcaacccgag ctgttcgtcg aagacgtgcg
     1921 cgcgttcttc gcgacggtgc ggtgagtcag ccgagcagcg gcttgcggtt ggcggtcatc
     1981 tcgtccagcg cggattgcat tgccgactgc acgcgttcgg cataggcgtc gggttcttcg
     2041 tccggctcgg cgtgaattgc ggtcagaacg cgggtgtcca atttggtggg cagcggaaga
     2101 tacggcagta agccgaccgc ccccacgttt accccccacg gcagcgaaac ggtgagcggt
     2161 aatgccttga tgcgcaacag tttgtcgacg cgcagcgccc gcgcaaggcg ttcgcctctc
     2221 gagatcacga gcagcgactc actggcgccg gccgtcacga tcggaacgat cggcacaccg
     2281 aattcgattg caagccgggc gaatccgctg cgcccgccga acacgacctg gttgcgctcg
     2341 tgccaggtct tggcggcgtc gagatcaccc ccggggaaga cgagcacgtg ctggccctcc
     2401 ccgaatgcct cccgggcgct gtcatggctt gcgggacgag cgccgaccgg ctcgatgaac
     2461 ggaccgacac ccagcgtcca ggcgagttgg tgggtcaggg ccgtgacggg ccggtcgagc
     2521 tcgagctgtt cgagggccgc gtacgtcgcg aacacgttca ggtcgaagat gccaccgaag
     2581 ccgtggttgg cgacgaagag caccggcccg ttcaatgcac tcgattcgac acggacgtcc
     2641 aaccggttgt aacggcgcac gaactcaatc cctaactgaa gcaatgccat tgccgccgta
     2701 gccatcgcat gggacggcga aacgtcgggg aggttcagct gttcttcgac cgcggcgagc
     2761 agcgcctcgg cgaactgctc gacgtcggtc tgatcgcgct gcttgcccga atcagtcatc
     2821 tatgcctcca ctcgcatcac gcccagctag gcttccacac ggcgctggcc gtgggtcaac
     2881 accctccgat ttcaagctcg cccagttcga gacggcagcc cgatcagtgg tgcgtcgcga
     2941 ggatgctgtg cccgccccag ctggcgcggg tatccgcgac gactgaggag ctgcagggga
     3001 cgcatcgaca cccaccaaca cctcattccg cccggctacc ggaatgcgtt gcgcaaagcc
     3061 ggcatcgacg aagccggcgg ccgtgccctg cccgactgga gcccggatgc ggcactccag
     3121 gcgatggccg cgctcgagtt ggcgactgcc atcgtgtcgg tatccgctcc gggcaccacg
     3181 tttcttccgg ttgcagccga tgctgctgcg ctcgcccgcg atctcaacga ttacagcgcc
     3241 gctctcgttg cgtcacagcc ggatcggttc gggttcttcg ccacggtccc gatgccccac
     3301 atcgacgagt cggttgccga ggcggtgcgc gcactcgatg gcctcgatgc cgacggtgtg
     3361 gtgctgctgg cgaacagcgc cggggtctat ctcggacaag acggccagga cgaactgtgg
     3421 gccgcgctgg acgagcgttc ggcggtggtg ttcgtccatc ccgccgaact gcccggacca
     3481 gcggtgcccg gcgtcgcgcc gtttgccgcg gactttctgc ttgataccac tcgcgcggcc
     3541 tacctattgg tgcgcaacgg cattcgccgc aggtatccca acatccggtt catcttgagc
     3601 catgccggcg gcttcgtccc gtacgcctcg caccgcatgg cattggcgat catgggcgac
     3661 accggccgca gccccgctga cagcctcgac gacttcgccg acttctattt cgacaccgcc
     3721 ctgtcgtccg gttcggcggc actgccgacc ctgttggcgt tcgccaaacc aggacacgtc
     3781 acattcgggt ccgactggcc gttcgcgccc gtcccggcgg cgaagctctt cgccgccgga
     3841 ttggagacct actcgggcat ggacgccggc acccacgccg ccatcgatcg cacgaacgcg
     3901 ctcgcgttgt tcccacgcct cggcacggcg ccggcaccca cgccggcgtc gcggattgac
     3961 gcggttcgcc atattgcgag tcgggtggtg atgcgggggg tcgctcggct catctctcgt
     4021 taagctgctg attcgaccgt tggcgggagg agccggcatg cccactgccc ttgaggtggt
     4081 cgacggcgtc gacctgaatg gcaagacctg cgtcatcacc ggggcgtcct ccgggctggg
     4141 gcgtgagtcg gcccgggcgc tggcgtcggc cggtgcgcag gtcgtgctcg cggctcgcaa
     4201 tcccgacgcg ctcgccgaat cgcagtcgtg ggtccgtgcg gaggtgccgg atgcggcgac
     4261 gacgacggtg catctggatc tcacctcgct cgccagcatc cgagcggccg ccgcgtcgat
     4321 ccaagagatc gctcccgcga tccacgtgct gatgaacaac gcgggcgtga tgttcacacc
     4381 gttcggccga accaccgatg gattcgaagc gcagttcggc acaaaccatc tcgaccactt
     4441 cgagctgacg cggttgctcg tcccccagct gctggccgcc ggtggggccc gggtggtgat
     4501 cctgtcctcc gacggtcatc tgatcagcga cgtcgactac gacgacccga actgggaacg
     4561 ccgcgactac gacaagttcc ttgcctacgg cgcgtcgaaa actgccaaca tcctgcacat
     4621 ggtcgagctc gaccgacgac tccgcgacgg cgcagtgcgc gcctattccg tgcatcccgg
     4681 catcgtcgcc accgcgctcg cccgctacat gacgcgcgac gacttcgcga agctcaacaa
     4741 cttcgggtca acttcggagt ccgagaatcc cgtcgacatc cgccgtgact ttctgacccc
     4801 cgaccaaggt gctgccacgc aggtgtgggc cgcggtcagc ccggaactcg ccggcctcgg
     4861 cgcgctgtac ctagccgact gccgagtgcg tgagaacgtc gcgccgtacg cgatcgacga
     4921 gcagcggtcc gcgcggttgt gggcactttc cgaatcgttg tgcggcaact gaagggcggt
     4981 cggccatgcg gtgggttacc tatcggagcg acgagggcga ccgggtgggt gtggtgccag
     5041 accagtcgat ctatgcggtt gacgccggca tcactctgat cgacctggtc gcccgcggcc
     5101 acgacggatt gcgtgccgcc ggagaagacg cgctgcgcag cccgtcggcg gtcctccggc
     5161 tggacgaggt ctcgctgcgg gcgccgattc ccacaccgcc ggctgtccga gacagcctgt
     5221 gcttcctcga ccacatgcgc aactgtcagc aagcgctcgg ggccggacgg gttctcaagg
     5281 acacctggta ccggatcccg gcgttctatt tcgcctgccc gtcaaccgtt ttcggacccc
     5341 acgacgacgt gccgacggca ccggggagcg cgtggcagga cttcgagttg gaaatcgccg
     5401 cggtcatcgg caccggaggc aaggacctgt ccgtggagga tgccgaacag gcgatcatcg
     5461 ggtacacgat cttgaacgac tggtcggcgc gcgaccttca gacgctggaa agccaactgg
     5521 gaatcggaca ggccaaaggc aaagacagcg ggatcactct gggaccctat ctggtcaccc
     5581 ccgacgaact cgccgcttac cgccgtgacg gtcggctctg cctggcggtg acggcgctgg
     5641 tcaacgacac cgaaatcggc tccggcacaa cagcagacat ggactggagc ttcgccgaag
     5701 tcatctcgta cgcgtctcgc ggtgtcacgc tcaacccagg cgacgtcgtg ggctctggca
     5761 cggtgcccac ctgcacgctc gtcgaacacc tggacctgac cgcgccggac gccttcgtcg
     5821 gctggctgca cgacggtgac accgtgacgc tgcgcgttca gggattgggc gagacccgcc
     5881 aaactgtcag ggccactccc ggcccgcgcc cactacggcc gcgccgaaac ccggacacca
     5941 tcgcgcgacc tgcccgggtg aaccgcgcgc cgactcgcat cccgtacgtg cgcgggttgc
     6001 ataacgtcgg cgagcgggta tgggcgtgga cgctgcccga cggcggatac ggttggagca
     6061 atgcgggttt gaccgcggga gaaggtgcgt cgcttctcgt cgacacgctg ttcgacctgc
     6121 atctcacccg cgagatgctc gccgcgatga g
//
LOCUS       DASWBF010000252         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1210200,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000252 DASWBF010000000
VERSION     DASWBF010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210200"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..791)
                     /locus_tag="VF874_09150"
     CDS             complement(18..791)
                     /locus_tag="VF874_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine reductase family protein"
                     /protein_id="HEX9176238.1"
                     /translation="MTDDDAAARFTRLDSLTTVDAEELPRLYAYPPDLTTCWVRANMI
                     ASVDGGATLEGRAGGLAGPGDRVLFKVLRELADVVLVGAGTVRTENYSGAQLSAASRQ
                     ARQARGQSEIPPIAVVTASGALDLGSRLFTNTETPPLVFTTTASFVATRGRLHGQAEV
                     LDASTSDPIAVDPAAVLAELAQRGLYRVLCEGGPTLLGDVVELNVLDELALTVAPVLV
                     GGGAPRVVTGQTPTTTELRTEHILSDAEGYLYLRYARTR"
     gene            complement(845..1381)
                     /locus_tag="VF874_09155"
     CDS             complement(845..1381)
                     /locus_tag="VF874_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9176239.1"
                     /translation="MEPVEVATPGTAEIEELAAVAARTFPLACPPTATPENIVEFIDT
                     HLSAERFAEYLADEDRVVLAAYEYGRIAGYCMLIRGFPEDDADVQQAVTARPTVELSK
                     IYVLPDSHGGAVSAALMQAALDRAAAMAATSVWLGVNQQNQRAQRFYAKHGFKINGTK
                     SFHLGAGVENDFVMVRAV"
     gene            complement(1369..1860)
                     /locus_tag="VF874_09160"
     CDS             complement(1369..1860)
                     /locus_tag="VF874_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176240.1"
                     /translation="MRRWFLGLFAVMVVARALVAPPASAAETPIGRLGDTLRVEAGNI
                     VADVTVNSVLPSDIPPGWGYPPRWPRSQVDRAQITVTAVETPTPASMSSRLSFRGVTQ
                     TGDQYVPRSTDAPDALQYALLNAPQGATVSGGVWWDCYRDLVSNVVLTDVKTGFRLAQ
                     WNL"
     gene            complement(1918..2346)
                     /locus_tag="VF874_09165"
     CDS             complement(1918..2346)
                     /locus_tag="VF874_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX9176241.1"
                     /translation="MKLDEHARALIGYGADATLVTLNPDGSPQVSVVWIALHTTPDGD
                     DEVVTAHLAEHKKVRNIRRANRVALTVLSTDRSGAMTPYLSMSRTASVEEGGAPELLR
                     DLAKTMIGHDAKFPPMDDPPPGFITHITIDRIGSVGPWTG"
BASE COUNT          376 a          793 c          848 g          387 t
ORIGIN      
        1 agagtagcga gcgggcgtca cctggtccgg gcgtagcgca ggtacaggta gccctccgcg
       61 tcggacagga tgtgctcggt gcgcagctcg gtggtcgtcg gggtctggcc ggtcaccacc
      121 cgcggcgctc cgccacccac cagcaccggc gcgacggtca gggccaactc gtcgagcacg
      181 ttcagctcga cgacgtcgcc gagcagcgtc gggccgccct cgcacaacac ccgatacagc
      241 ccgcgctgcg ccagctcggc cagcaccgca gccgggtcga cagcgatcgg atcgctggtc
      301 gaggcgtcga gcacctcagc ctgcccgtgc agccggccgc gggtggcgac gaacgacgcg
      361 gtggtggtga agaccagcgg cggcgtttcg gtgttcgtga acagccggct gccgaggtcg
      421 agagcacccg acgccgtcac cacagcgatc ggcggaatct cggactggcc gcgcgcctgc
      481 cgagcttgcc gggacgccgc gctcaactgg gcgccggaat agttctcggt gcgcaccgtc
      541 ccggcgccga cgagcacgac gtcggcgagc tcgcgcagca ccttgaacag cacgcggtcg
      601 ccggggcccg caaggccgcc tgcccggccc tcgagggtgg cgccgccgtc gacgctggcg
      661 atcatgttcg cccgcaccca gcacgtcgtc agatccggcg ggtaggcgta gagccgcggc
      721 agctcctcgg catcgacggt tgtcagcgag tccagccgcg tgaaccgcgc ggcagcgtcg
      781 tcgtcggtca cgggaacgat tgcagcacgg ctgatcgcat taacgaagct ggcccacacc
      841 gtccctagac tgcgcgcacc atcacgaagt cattttcgac gccggcaccg agatgaaatg
      901 acttcgtgcc gttgattttg aatccgtgct tggcgtagaa gcgttgcgcg cgctggttct
      961 gctggttgac gcccagccac actgacgtgg cggccatggc cgcggcgcgg tcgagggcgg
     1021 cctgcatcag cgcggccgaa actgcgccgc cgtggctgtc gggcagcacg tagattttcg
     1081 acagctcgac agtggggcgc gcggtgaccg cttgctggac gtcggcgtcg tcctcgggaa
     1141 aaccacgaat cagcatgcag tagcccgcaa ttcggccgta ctcgtaggcg gccagaacca
     1201 cgcggtcttc gtcggcgagg tattcggcga accgctcggc cgacagatgg gtgtcgatga
     1261 actcgacgat gttctccggc gtcgccgtgg gcgggcacgc cagcggaaac gtgcgtgcgg
     1321 caaccgcggc cagttcctcg atctcggctg tgcctggggt cgcgacctct acaggttcca
     1381 ctgggccagg cggaagccgg tcttcacgtc tgtcagtacc acgttggaga ccaagtcgcg
     1441 gtagcagtcc caccacaccc cgccgctcac ggtggcaccc tgcggcgcat tgagcagcgc
     1501 gtactgcagc gcatcgggag cgtcggtact gcgtgggacg tactggtcgc cggtttgggt
     1561 gactccgcgg aacgacaacc tcgacgacat cgaggcgggg gtgggcgtct cgaccgcggt
     1621 gacggtgatc tgagcgcggt ccacctggga ccgcggccac cgcggtgggt agccccatcc
     1681 gggcggaatg tccgacggca gaacactgtt cacggtcacg tcggcgacga tgttgcccgc
     1741 ttccacgcgc aaggtgtcac cgaggcggcc gatcggcgtc tcggctgccg acgcgggagg
     1801 tgcgacgagc gcgcgggcga cgaccatcac agcgaacagt ccgaggaacc agcggcgcat
     1861 cttcccctcc ttagctgcct ccgcatgatg tcacaggtca gccccggaaa tcacgggtta
     1921 accggtccac gggcccacgc tgccgattct gtcgatcgtg atgtgcgtga tgaagccggg
     1981 cgggggatcg tccatcggcg ggaacttggc gtcgtgtccg atcatcgtct tggccagatc
     2041 gcgcagcagt tcgggtgctc cgccctcctc gacgctggcg gtgcgggaca tggacaagta
     2101 cggcgtcatg gcgccgctgc ggtctgtgga caggaccgtg agcgcgaccc ggttggcgcg
     2161 acgtatgttg cgaaccttct tgtgctcggc gaggtgggcg gtgaccacct cgtcgtcgcc
     2221 gtcgggcgtg gtgtgcaacg cgatccagac gacgctgact tgtgggctgc cgtcggggtt
     2281 gagcgtgaca agggttgcgt cggcgccgta tccgatcaag gcgcgcgcat gctcgtcgag
     2341 tttcatgagc cggacattac gccggggcgg tccccgtggt gaacggctcg agcgcggtga
     2401 ccac
//
LOCUS       DASWBF010000253         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1270997,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000253 DASWBF010000000
VERSION     DASWBF010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270997"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..682
                     /locus_tag="VF874_09170"
     CDS             35..682
                     /locus_tag="VF874_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9176242.1"
                     /translation="MLKRLLNNRCSPVNLRCISTTTAALTPIGLTTTTPPLCRVGWWC
                     QAGEPDRRVSDTNVTGAALATAAAVPHLTASAGKAVYLSSDAGTFGPPWPCLGAYGVS
                     KAALARLVEAWRAEHPDISFTCLIVGECGGGEGDGQTGMTTGWDPELAVRAYPLWVSR
                     GCMPGKLMPVEDLVEVVHTILQTDAATSMPLVIARGAPDSPAGVSGLRSTLTRAA"
     gene            complement(766..1938)
                     /locus_tag="VF874_09175"
     CDS             complement(766..1938)
                     /locus_tag="VF874_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9176243.1"
                     /translation="MRRFTVHERRARLACRHFLTAERTDGAIPALTAAMLGLHATDPA
                     TPYLSLWARMRGFTVEDLDRELYERRSVVKHLAMRRTLWLVRGEDLTAIQPGASDRVA
                     GNERRRLVTDAQKAGVAANGDAWLDRASEAVLRYLADNGPAGATEVRAALPELHGTYD
                     PAPGRRWGGETPLAPRVFTVLSVRGDIVRGPNDGTWTVSRPRWVTTEDWLGAASDVMP
                     AEKARAELTRRWLRAFGPATLADIKWWFGTTLTMARQALHDVGAVGVELDGAPGVALP
                     DDLEIEPDVGPWCALLPGLDVTTMGWADRDWYVGPYRTSVFDANGNAGPTAWWNGQVV
                     GGWCQDSAGRVQLQLLEDPGRDGRRVLQRRADELTDWLGGTRVSPRFPTPLSKAGA"
     gene            complement(1940..2188)
                     /locus_tag="VF874_09180"
     CDS             complement(1940..2188)
                     /locus_tag="VF874_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176244.1"
                     /translation="MPDEPSPTDEPDVDPGYSESGVPTFESVREKIETRFGTSLGAAE
                     LDAETPEGRTAEEQYEARQRAAAERLAQIGESMHRDDA"
BASE COUNT          360 a          810 c          800 g          402 t
ORIGIN      
        1 cacgtcaggg gctttccgag gtctttcgtt agccctgcta aagaggttgt taaacaatcg
       61 gtgctcccct gtcaatcttc gttgcatctc gacaacaaca gcagcgctca caccaattgg
      121 attaactacc accaccccac ccctgtgccg ggtggggtgg tggtgccagg ccggggaacc
      181 tgacaggcgc gtctccgata ccaatgtcac gggcgcggcc ctggcaacag cggcggcggt
      241 gccgcatttg acggcatccg cgggcaaggc ggtctacctc tcatccgacg caggtacatt
      301 cggcccgccc tggccctgcc tcggcgcgta tggcgtcagc aaggcagccc tcgcgagact
      361 cgtggaggcg tggcgcgccg aacatccgga tatcagcttc acgtgcctga tcgtcgggga
      421 gtgcggcggc ggcgagggcg acgggcaaac gggaatgacc accggctggg atcccgaact
      481 cgctgtgagg gcctacccgc tctgggtgtc gcgtggttgc atgccgggca agctgatgcc
      541 cgtcgaggac ctcgtcgaag tggtgcacac gatcctgcag accgatgcgg ccacgtcgat
      601 gccgctcgtg atagccaggg gtgcgccgga tagccctgcc ggggtttctg gactccgatc
      661 gaccttgacg cgggcggcgt agatcgagtc tgcggtcacg gcgccgagtg tgcgctgagg
      721 gcgggatctt gcgaattcct cgccctgtgc gcagtttcgg cgaagtcacg caccggcttt
      781 cgacaggggt gttgggaacc gaggagatac ccgcgtgccg cctagccaat ccgtgagttc
      841 gtcggctcgc cgctgaagca cccgtcggcc gtcgcggccg ggatcctcga gcagctgcag
      901 ctggactcga cccgcgctgt cctggcacca gccgccgacc acctggccgt tccaccacgc
      961 cgtaggccct gcgttgccgt tcgcgtcgaa aacgcttgtg cggtaaggcc cgacgtacca
     1021 gtcccggtcg gcccagccca tcgtcgtcac atcgaggccc ggcagcagtg cgcaccaggg
     1081 cccgacatcc ggctcgatct ccaggtcgtc aggcagcgcg acgcctggcg cgccgtcgag
     1141 ttcgaccccg accgccccga catcgtgcag cgcctgccgt gccatggtca gcgtcgtgcc
     1201 gaaccaccac ttgatgtcgg cgagcgtcgc cggaccgaac gcgcgcagcc accgccttgt
     1261 caactcggcg cgggcctttt cggcgggcat cacatcggat gccgcgccga gccaatcctc
     1321 ggtggtcacc caccggggac gcgagacggt ccacgtgccg tcattcgggc cgcggacgat
     1381 gtcgccacgc accgatagaa ccgtgaatac ccttggtgcc aagggagttt cgccacccca
     1441 acgcctgccc ggcgcgggat catacgttcc gtgcagctcc ggaagcgcgg cgcgcacctc
     1501 ggtggcgccg gccgggccgt tgtcggccaa gtaccgaagc acggcctcgc tcgcgcgatc
     1561 aagccaagcg tcgccgttcg ccgccacccc ggccttctgg gcgtcggtga cgagccggcg
     1621 tcgttcgttg ccggcgaccc ggtcgctggc accgggctga atcgcggtca ggtcttcgcc
     1681 ccgcaccagc cacagcgtgc ggcgcatcgc gagatgtttg accaccgaac gtcgctcgta
     1741 gagttcgcgg tcgagatctt caacggtgaa gccccgcatg cgggcccaca gcgacagata
     1801 cggcgtcgca ggatcggtcg cgtgaaggcc gagcatggcg gcggtgagcg cgggtatggc
     1861 gccgtccgtg cgctcggcgg tcaggaaatg gcggcatgcc agacgagcgc ggcgctcgtg
     1921 gacggtgaac ctgcgcaccc taggcgtcgt cgcggtgcat ggattcgccg atctgagcga
     1981 gacgttcggc cgccgcgcgc tgacgcgctt cgtactgctc ttcggcggtc cggccctcgg
     2041 gcgtctcggc gtcgagttcc gcggccccca gcgaggtgcc gaacctggtc tcgatcttct
     2101 cgcggacgga ctcgaacgtc ggcaccccgg actcgctgta cccggggtcg acgtccggct
     2161 cgtcggtcgg agacggttcg tcgggcatgc cgcaacggta cctctgcgtc aaccgagggc
     2221 gcggtcgtgc tggcactgtc aacttgagcc aacctaattg gccgatgtga tgattcgggg
     2281 tgatgagccg tggcttggac gaaacgtcct gtcccagtcg ccgattcagg cggcggccgt
     2341 ggtcgtcgcc ccggtgatct cgttccagac cg
//
LOCUS       DASWBF010000254         5202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_684012,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000254 DASWBF010000000
VERSION     DASWBF010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684012"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..674)
                     /locus_tag="VF874_09185"
                     /pseudo
     CDS             complement(33..674)
                     /locus_tag="VF874_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            1008..1613
                     /locus_tag="VF874_09190"
                     /pseudo
     CDS             1008..1613
                     /locus_tag="VF874_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
     gene            complement(1605..2294)
                     /locus_tag="VF874_09195"
     CDS             complement(1605..2294)
                     /locus_tag="VF874_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEX9176245.1"
                     /translation="MSRFLCAAALSAGVVSAATSAIPSAAADGCSDVEVVFARGMGDP
                     VGPGPLGAAFADSLRSKLAGRSVGVYGVNYPADVNFLRVSDGVADATNHIQWLAANCP
                     ATKVVLGGFSEGAAVVDILAGANLPGLNAVPALGAIPYVNEVLPAIGSAAPLPADLND
                     RVAAVAVFGNPLGKVSGPLNTASPVFGARTIDLCYPGDPICSEGKNMTLHHQYVPGDT
                     DQAATFVAGLL"
     gene            2493..3311
                     /locus_tag="VF874_09200"
     CDS             2493..3311
                     /locus_tag="VF874_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX9176246.1"
                     /translation="MPFGQIAVLGAIAGATIFLGLPLGRVNAAVPRLKTGLNALATGV
                     LLFLFWDVTSHAWEPVDDALSDHDLGAALSHGAVLAVTLGVGLIGLVWVDRFAARRSD
                     VRTGGPGAATLAELQTPLWATAAGRLAVMIAVGIGLHNFAEGLAIGNSAASGEISLAV
                     MLVIGFAVHNATEGFGIVAPLTGQRPPWAFLALLGVIGGGPTFIGTLVGQRFVNTTVS
                     VAFLGLAAGSILYVVIELLAVARKSDLKMITTCAISAGLVLGFTTDAVITAAGV"
     gene            3413..4339
                     /locus_tag="VF874_09205"
     CDS             3413..4339
                     /locus_tag="VF874_09205"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9176247.1"
                     /translation="MSGLTYTEAVVVGGFQGITELFPVSSLGHSVLVPALAGGRWAED
                     LNVSAPESPYLAFIVGLHVATAAALLVFFWRDWVRILAGFGTSLRHRRIHTPAERLAW
                     LIVVATIPVGIAGVALEHLFRTTLGRPVPAAAFLLANGAVLLAGERLRRRVRVPAGVA
                     ATGDAGGSGQHSGGLDESELRLAQMPVGKGLFIGSAQILALLPGISRSGVTMVAGLLR
                     GLNHDDAARFSFLLATPVILAAGVFKIPDLFGPLGDGIGGQVLAGSAASFVSAYFAVR
                     FLTRYFHTRTLTPFGVYCVAAGAGALIWLTVR"
     gene            complement(4352..5020)
                     /locus_tag="VF874_09210"
     CDS             complement(4352..5020)
                     /locus_tag="VF874_09210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9176248.1"
                     /translation="MLPPPPASARVPLPGPGVLSVLPGDGDLLALTVDDGVNTDVVRL
                     YTQFAKDTGIRLTFFVNGTYRSWTDNAALLRPLVDSGQIQLGNHTWSHPDLTTLSAAR
                     IGDEITRNHTFLKNTFGVDAAPYLRAPYGKHNAAVRAVAADLGYTVTTMWSGSLADST
                     VVAEDFIVKMADTYFRPQNIVIGHLNHPAVTHVYSRLVDIIGSRRLRTVTFNDVFIRP
                     YDNK"
BASE COUNT          765 a         1660 c         1815 g          962 t
ORIGIN      
        1 gacccgtcgc gtgataggcc gtcggctacg aactagtcgc gcggctcatc gtgaggtggg
       61 tgagtcttcc gcgttcgctg cagtctgtcg aggatcgacc tgtgatcggt ggtgctgatg
      121 aagtcctcga tgaggtccac cacctgggtt gggcactcca cgtgggggaa gtgccccaca
      181 ccggcgagca cctcaagtcg aatccccggc cgggcggcat acgccgcgta accatgctcg
      241 acggggatga tccggtcttg gtcaccccaa atcgccattg tgggtagatc gggcgggagc
      301 tcatccagcc gattcagagc gctcactgcc tgtccgcggt agtcgaccac tgcccgcagc
      361 gtccgtagga acggttgctg cgtctgccga tccgacagcg acgagtaggc gttccacagc
      421 tcgccgcccc gcggtgactg gattcctgcg gaggcgaacc acgatcgcag tttgttgccg
      481 agggtcaaca ccggccgcgg cgcgatcacc ggcagcacga actctgctcc gggtgccgag
      541 agaatccgca gtgtccggcc caagtcgggc ccgaggccgc cgctgctgat gagaatcagt
      601 cgctggcagt agtcggggtg ctgataggcg aactgcattg ccacgccgcc gccgagcgaa
      661 tgaccgacaa ccgtcactcc ccaccagttc tgcggaaagc cgtgcaccag catcaccggc
      721 gcgccgtcgg ccggcccggc gtcggcgaca tgaatcgtga catcaccgcc cagatcgtca
      781 aaccggtgct ccacgcctcc gagttccgga aataaatcaa cgttcacccc tgctcgcttt
      841 cggtcggagg actcgatcgg gcggggtccc cgcctttgca ctatgtctgt atcggttcgc
      901 gtgtgtggtc ggatcacccc tgttcagtca ctcaggcggc tcgtcggaaa ccgtattgac
      961 ggctggcaac tgcccgcggt ttccaggcgg tacatggtca gctgtaggac agctcgcagc
     1021 tgtccatccc cagggaccgg gtcgacgatg agcagctgca tgccccctca cactggaacc
     1081 tggccttcat ccgccggttc atgctggtct tcggaccgat cagtgcgctg ttcgacttca
     1141 tgacgttcgg gctcatgctc ggtgtgctgc acgccggagc cgtcgagttc caaaccggct
     1201 ggttcgtgga atcccttgcg acacaaacgt tgatcatctt cgcgatccgc acccgcaagg
     1261 tgccgttctt ccgaagccgg cccggcggct tgttgacggt gaccagtctg tcggtggtgg
     1321 ccatcggcgc gctgctgacc atctcgccgc tggcggccac gctgggcttc acaccgctac
     1381 cgtggccgtt ctttgccgtc ctcgtcggct ttgccgtcgc ctaccaggtg ctggtcgagt
     1441 tcgccaagaa gatgttctac gccgagccga tccggctggc cggccagccg gatcgcaccc
     1501 gagggcgtga tcaccgcatt caccgccgcg ccgcgcgctt ctcccaccgc aacggggtga
     1561 ccgcaccggg atccgtgacg tcgtcgccgg cttccggtgg cgggctaaag taggccggcg
     1621 acgaacgtcg cggcctggtc ggtgtcaccg ggcacgtact ggtggtgcag ggtcatgttc
     1681 ttgccctcgc tgcagatggg gtcgcccgga tagcacaagt cgatggtgcg ggcgccgaac
     1741 accgggctgg ccgtgttgag cgggcccgac accttcccca gcgggttgcc gaacaccgcc
     1801 accgcggcga cacggtcgtt gaggtcagcg ggcagcgggg cagcggagcc gatggcgggc
     1861 aggacctcgt tgacgtaagg gatggcgccg agggctggga ccgcgttcaa cccgggcagg
     1921 tttgcgccgg ccaggatgtc gacgaccgcc gctccctcgg agaagccgcc gagcaccacc
     1981 ttggtggcgg gacagttggc ggccagccat tggatgtgat tggtcgcgtc ggcgacaccg
     2041 tcggagaccc tgaggaagtt gacgtcggcg ggatagttga cgccgtacac cccgacggac
     2101 cgaccggcga gcttcgaccg cagcgagtca gcgaacgccg cgcccagcgg cccgggtccg
     2161 acgggatcac ccatgccgcg ggcgaacacc acctcgacgt ccgagcagcc gtcggccgcc
     2221 gccgacggaa tcgctgacgt tgcggcggat acgacaccgg cgctcagcgc tgcggcgcaa
     2281 agaaaacgac tcatcagacg tgcagacgcg gcggggaacc tcatttacgc agcataggcg
     2341 aacgcttcgg ctctgtcggc tatgcgtcag ccgagatcga tgccggcagc aaatgagtgc
     2401 gcagtgcttg cagtgttcgc tccgcccacc acatgattag ctcaccctta cctgggtgta
     2461 gcgtgcctac tgttgtcggt gagggagcag caatgccgtt cggtcagata gcggtactgg
     2521 gggcgatcgc gggcgccacc atcttcctcg gtctgccgct gggtcgggtc aatgccgcgg
     2581 tgccgcggct gaagaccggc ttgaacgcgt tggcgaccgg cgttctgctc ttcttgttct
     2641 gggatgtcac cagccacgcc tgggagccgg tcgacgacgc gctcagcgac cacgatctcg
     2701 gcgctgctct gagtcacggc gcggtgctgg cggtgaccct gggtgttggt ctgatcggcc
     2761 tggtctgggt ggaccgattc gccgcgcgcc gcagcgacgt gcgcaccggc ggtcccggcg
     2821 cggcgacgct ggccgagctg cagacgccgc tgtgggccac ggcggcgggc cggctcgccg
     2881 tcatgatcgc cgtgggcatc ggcctgcaca atttcgccga ggggttggcg atcggcaact
     2941 ccgcggcgtc cggagagatc agcctggcgg tgatgctggt gatcggcttc gcggtgcaca
     3001 acgccaccga ggggttcggg atcgtcgccc cgctcaccgg tcaacgaccg ccgtgggcgt
     3061 ttctggcgct gctcggggtg atcggcggcg gccccacctt catcgggacg ctggtcggcc
     3121 agcgtttcgt caacaccacc gtgtcggtgg cgtttctggg tctggcggcc ggctcgattc
     3181 tgtacgtggt gatcgagttg ctcgcagtcg cccgcaagtc agatttgaag atgatcacca
     3241 cctgcgcgat ctccgcgggc ctggtgctcg gcttcaccac cgacgccgtc atcaccgccg
     3301 cgggcgtcta gcctctgctg gccttcgagt tcacgcggct ccgggcctct cagcgacatc
     3361 tcagctatgg ctgcttagcg tgaaggccgc agcggcagga aagggagacg acgtgagtgg
     3421 gctgacctac accgaagcgg tggtcgtcgg cgggtttcag gggatcaccg aactgtttcc
     3481 ggtgtccagc ctcggtcact cggtgctggt gccggcattg gctggcggcc ggtgggccga
     3541 ggacttgaac gtgtcggcgc cggagtcgcc gtatctggcg ttcatcgtcg gtctgcatgt
     3601 ggccaccgcg gcggcgctgc tggtgttttt ctggcgggac tgggtgcgaa ttttggccgg
     3661 cttcggcacg tcgctgcggc accgccgcat acacacgccg gctgagcggc tggcctggct
     3721 gatcgtcgtg gcgaccattc cggtcgggat cgccggtgtg gcgttggaac atctgttccg
     3781 caccacgctg ggcaggccgg tgcctgccgc ggccttcctg ctggccaacg gtgcggtgct
     3841 gctggccggg gagcgtctgc gccgccgggt ccgcgtccct gccggtgtcg cggccaccgg
     3901 cgatgctggg ggtagcggtc agcattccgg cgggctggat gagtccgaac tacggctggc
     3961 gcagatgccg gtcggaaagg gcctattcat cgggtcggcg cagatcttgg cgctgcttcc
     4021 cgggatcagc cgctccgggg tgacgatggt ggccgggttg ctgcgcgggc tcaaccacga
     4081 cgacgccgca cggttttcat tcctgctggc cacaccggtg attctggccg ccggggtgtt
     4141 caagattccc gacttgttcg ggccgctggg cgacgggatc ggtgggcagg tgctcgccgg
     4201 cagtgcggcg tcgttcgtca gcgcctactt cgcggtgcgt ttcttgaccc gctactttca
     4261 tacccgcacg ctgacaccgt tcggtgtcta ttgcgtggcc gccggtgccg gcgcgctgat
     4321 ctggttgacc gtgcgctgag ccgatgaccg gctacttgtt gtcgtacggg cggatgaaca
     4381 cgtcgttgaa ggtcacggtg cgtaggcgcc ggctaccgat gatgtcgacc agccggctgt
     4441 agacgtgggt gaccgcggga tggttgaggt ggccgatgac gatgttttgc gggcggaagt
     4501 aggtgtcggc catctttacg atgaagtcct cggcgaccac ggtggagtcg gccagcgagc
     4561 ccgaccacat ggtggtgacc gtataaccga ggtcggcggc gaccgcccgc accgccgcgt
     4621 tgtgtttgcc gtagggcgcc cgcaggtagg gtgccgcgtc gacgccgaac gtattcttca
     4681 aaaacgtgtg attgcgggtg atttcgtcgc cgatccgtgc ggcgctcagc gtggtcaggt
     4741 cggggtgtga ccaagtgtgg ttgcccagct ggatctgccc ggaatccacc aagggccgca
     4801 gcagcgccgc gttgtcggtc catgaccggt aggtgccgtt gacaaagaag gtcaaccgga
     4861 tgccggtgtc cttggcgaac tgggtgtaga ggcgcaccac gtcggtgttg acgccgtcgt
     4921 cgacggtcag cgccagcagg tcgccgtccc caggcagcac cgacagcacg cccggcccgg
     4981 gcagcggaac tcgggcgctg gccggcggcg ggggcagcac cgatccaggg gcacatgaag
     5041 tgggccgagg cgatgccgtg accgcgcgct cgctctacat ccctatcgat acttcgcttc
     5101 caccggtgag ccgctggcag gtcgccgaaa tcgctgctcg cgcggtgcgt gggtggtgct
     5161 ggagaagtgc tcggcgatcg cggcggcgat ctcgtagagg cg
//
LOCUS       DASWBF010000255         7702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_946399,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000255 DASWBF010000000
VERSION     DASWBF010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946399"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VF874_09215"
     CDS             complement(<1..1144)
                     /locus_tag="VF874_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9176249.1"
                     /translation="MPSASAPVRCMAAATTAVALLAANMLVPAAAIAIEPPMIDPAAL
                     PRDGVPGPDQEMKQDNTCAAPLTVAVPDVVQPAPGNVMLAVPQAWKFSTGAGVRVGLI
                     DTGVTPNPRLPALSAGGDYVMGDANGGLSDCDSHGTVVASIIAAQPSDPATRPAPMPP
                     GASAPPPPAPPPASPFPTTPPPPPPKPSTVTVTTPAPPPPPPPEEPPPEEPPPPDAPA
                     AGPGDEEGDPPVPPPPPGSPDGVVGVAPDVTLISVRQSSSAFGPAKPAAGDEGERREK
                     AGDIATLARAIMHLANLGVDVMNISVTACVNAAAPIDQAALGAAVRYAAVDKDVVIVA
                     AAGNVGEENSCGQNPVFDPLRPDDPRDWHEVSTIVTPAWYSDYVLAV"
     gene            complement(1161..2669)
                     /gene="eccD"
                     /locus_tag="VF874_09220"
     CDS             complement(1161..2669)
                     /gene="eccD"
                     /locus_tag="VF874_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HEX9176250.1"
                     /translation="MTTFRAPPAHAAAPPAVPPSCRVSVLAGDSHQIDLVLPAAAPLS
                     TLVDPTVAVINKVLRGRGAPELPTATYEFARAAGMSVLASDVSLTGHNIVDGDMLVLM
                     PAGAAERYGPVVENVSTALAQYASEHFVRVTAETAIAVAVGIMGAALAVAAALLWRLR
                     WATESSLLVTALLAGAAVVLVGTAVLATRLGAARVVSDGAAWGAIALATLAATTMPSG
                     LGPGAPHAFLAAVTAVAGTWLLVRLTGRYWSAAAAVITAGVGVIAASAIRMYWAVPGP
                     RLAVGMSVAVLVAVATAPAVALWLAKVPRQTFGSITGRDIFARAPGQPEDTVSPVEAG
                     PPADATLTGEQVAAAARRSNTVLTGVLLGIAALTVPSALVATTPGDAQQWAQLVVVGT
                     VAMIFILRARAFRDRRHAIILVVAATAAMLGAAARYGFHAAATDTGAALTAGGAAIGI
                     ALAGLLAAAVVPPRVFSPPVRKVVEYTEYVLLAVVVPFAAWAVGLLHYIRYH"
     gene            complement(2698..4119)
                     /locus_tag="VF874_09225"
     CDS             complement(2698..4119)
                     /locus_tag="VF874_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX9176251.1"
                     /translation="MSGRRRRRWGHSRPPTPQRKSPSSPQSRLLCSPQSRAGPTPQTR
                     APARPQEGAPQQQWPQTPEPGGPTTGMHYRTAPANYPPPSRQPGPPGQQAQHQQPAPP
                     SGPPPSSESMRMFNPGRPDRPSTWPPAREQAGPGPRPGGGWQPTPRWQEPPTPPTNWS
                     YVDSIRTSELVPTRKPPPGRGWRRAVYKATFGTVNLGQSPDELRQAALEAKIRSVLRG
                     HYKIGVLGKGGVGKTTIAAGVGSILAEVRQDDRVVAIDADTSFGKLGSRVDPHAAGSY
                     WELAADDHLDTFADVRSRVGNNSSGLFVLAGEASTARRRVLDPAIYREAAARLDRHFT
                     ISIIDCGSTMDSPVTQEVLRDLDALIVVSSPWVDGAAAAGQTMEWLANRGLTGLLHRT
                     VVVLNDSDGHADKRTRNILQQQFASRGQVVIEVPFDTHLRPGGVIDVGTEMSPKTRRK
                     LFEIAAAIAEHFVATTDAPRERR"
     gene            complement(4169..5665)
                     /gene="eccB"
                     /locus_tag="VF874_09230"
     CDS             complement(4169..5665)
                     /gene="eccB"
                     /locus_tag="VF874_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HEX9176252.1"
                     /translation="MALNLASGLQVSAHFFWQRRNAAALSHHGVRMEFDPVQRQRASL
                     LLGFIFALLGVALMFLLSWFKPAGHVGESTILADRDTGAVFVLVDGRLHPALNLVSAR
                     LIAGQAANPTFVKASELAKYPQGPTVGIAGAPMAMPLRTGDASQWAICDTASPGKVSA
                     SSVVTAIGGPLTIGERAQQLRPPLAVLVTHNGATYVVWDDQRSQVDLSNKAVALALGV
                     DSAAPRPVTISNALFDALPATEPLTTPAVPNAGAPGGWNLAPGAVVGSVLSVRDLETG
                     DDNFYVLLTDGVQRISPFVATLLRSANSFGNPLPLAVAPDTLTHVPVVNPLPVGYYPT
                     TRLTFVDTGANPVTCVAWAKGSTDRSAVTTVLSGKGLPIPIGSDSRLLQLVKDARGPA
                     TIEADQVYMAPGATNLVMTTSASPEATSRESLWWISDQGVRYGISLDDASLRSLGITP
                     GSARQAPWPLMRVFAVGPALTRADALTQHDTVSGPAEAALLGKPNDNP"
     gene            complement(5716..6576)
                     /locus_tag="VF874_09235"
     CDS             complement(5716..6576)
                     /locus_tag="VF874_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESX secretion-associated protein EspG"
                     /protein_id="HEX9176253.1"
                     /translation="MADLTTSPAGIWVLQALLGVETMPIALRLRPFIPSIDSGPTVAT
                     MVGERPLTQTAEYASLVAAGVIDPDGRVDDAVRDWMAVVGRPEREVVVVIRRPVPDSD
                     GAVQERTLSVCRRSRWLAMIARNGDELVLAPVGESAAADQQIELICDAVLHAVGDAEP
                     AAIDGVNVPTKALQSALTATHGQDRWAVGTALARLGLSPDQVAVLVAGTRMNESAMAV
                     VTVVDHGATRYVHPAVLTVIDTEHGRVSVSVTYTTGPDGSGWTSVWPTTPGALRQDLA
                     RLLSVPRAAG"
     gene            complement(6666..6926)
                     /locus_tag="VF874_09240"
     CDS             complement(6666..6926)
                     /locus_tag="VF874_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176254.1"
                     /translation="MLPDYVQSISTASAQLEDVRSQAQNVLAHVRSVFDSRGGGAFEQ
                     AQMMINSGIDAGKEVIMRHSQVVSNANDDMIATDISAANSFG"
     gene            complement(6977..7294)
                     /locus_tag="VF874_09245"
     CDS             complement(6977..7294)
                     /locus_tag="VF874_09245"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HEX9176255.1"
                     /translation="MADSQLLVTPDQMRSAAQQLEQLRDQANATLQNYLNAAHDVHSS
                     QGWFGLAASTNITTTEEIHNAQMKLTTQWGELINTLRQAADNYEHQEQSSQSTIAGVP
                     NSV"
     gene            7492..>7702
                     /locus_tag="VF874_09250"
     CDS             7492..>7702
                     /locus_tag="VF874_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176256.1"
                     /translation="MPPVIAGCADVATSGTVAAGLADQPVGSFGGPGALKLLVGRVKL
                     DITALPGNPAPPAPRAAASVPAAIGP"
BASE COUNT         1173 a         2638 c         2708 g         1183 t
ORIGIN      
        1 cgaccgccag cacgtagtcg gagtaccagg cgggggtgac gatcgtcgac acctcgtgcc
       61 agtcccgcgg atcgtcgggg cgcaacgggt cgaacaccgg gttctggccg catgaattct
      121 cttcgcccac attgcccgcg gcagccacga tcacgacgtc cttgtcgacg gccgcgtacc
      181 gcaccgccgc gccgagcgcg gcctgatcga tcggggcagc cgcgttgacg cacgcggtga
      241 ccgagatgtt catgacatcc acacccaggt tcgccaggtg catgatcgcc cgtgccaaag
      301 tggctatgtc cccagccttt tcgcgccttt caccttcgtc gccggcggcg ggtttcgcgg
      361 gaccgaacgc ggacgaggac tgacgcaccg aaatcagagt cacgtccggg gcgacgccga
      421 ccacgccgtc gggactgccc ggcggcggcg gaggcaccgg cggatcgccc tcttcgtcgc
      481 ccggacccgc cgcgggcgca tctggcggcg gcggctcttc gggcggcggt tcttccggcg
      541 gtgggggcgg cgggggagcg ggcgtagtga ctgtcaccgt cgacggctta ggcggcggcg
      601 gtggtggagt cgtggggaac ggcgaagccg gaggcggcgc aggcggcggc ggcgccgatg
      661 caccgggagg catcggagca ggccgggtcg cgggatccga cggctgagcg gcgataatcg
      721 acgcgacgac cgtgccgtgc gaatcgcagt ccgacagccc gccattcgcg tcgcccatca
      781 cgtaatcgcc gcccgcggaa agcgccggaa gccgcggatt cggcgtcacc ccggtgtcga
      841 tcaagcccac cctgacacca gcgccggtgg agaacttcca tgcctgcgga acggccagca
      901 tcacgttgcc cggcgcgggc tgcacgacgt ccggcacggc cacggtcagc ggcgccgcgc
      961 aggtgttgtc ctgtttcatc tcttggtctg ggccgggcac gccgtcccgc ggcagcgccg
     1021 cggggtcgat catcggtggt tcgattgcta tcgccgcggc gggcaccagc atgttggcgg
     1081 ccaacagcgc cacagccgtg gtggcagcgg ccatacagcg gacgggtgcg ctggcggagg
     1141 gcatcagtga tatcggatca ttagtgatat cggatgtagt gcagaaggcc gacagcccag
     1201 gcggcgaacg ggacgacgac cgccaacagc acgtactcgg tgtactcgac gaccttgcgt
     1261 acaggaggag agaacaccct cggtggcacg accgccgcgg ccagcagacc agccagcgcg
     1321 atgccgatcg cggcgccgcc cgcggtcagc gccgcgccgg tatccgttgc ggcagcgtgg
     1381 aatccgtaac gcgcggcggc accgagcatc gcggctgtcg cagcgactac gaggatgatc
     1441 gcgtgccgac ggtcccgaaa tgccctggcg cgcagaatga aaatcatcgc cacggtgccg
     1501 accaccacca gctgcgccca ctgctgggcg tcacccggtg tcgttgcgac gagcgccgac
     1561 gggaccgtca gcgcggcgat cccgagcaga acgccggtca gcaccgtgtt cgaccgccgc
     1621 gcggccgcgg ccacctgctc accggtcagc gtggcatcgg cgggcggccc cgcttcgacc
     1681 ggcgacaccg tgtcctcggg ctggccggga gcgcgcgcga agatgtcgcg gccggtgatc
     1741 gacccgaagg tctggcgcgg caccttggcc agccacagcg ccaccgccgg cgccgtcgcc
     1801 accgcgacca gcaccgcgac cgacatcccg accgccagtc gcggcccggg caccgcccag
     1861 tacatgcgga tcgccgacgc ggcgatcacc cccacgccgg ccgtgatcac cgcggcggcg
     1921 gccgaccagt accggccggt caaccgcacc agcagccaag ttcccgcgac cgcggtgacc
     1981 gcggccaaga acgcgtgagg tgcaccgggg cctaaacccg acggcatcgt cgtcgccgca
     2041 agcgtcgcca acgcgatcgc cccccacgcg gcgccgtcgc tgacgacccg ggccgcgccg
     2101 agtctcgtcg ccagcaccgc ggttccgacc aggacaacgg ctgcgcctgc gagcagggcc
     2161 gtgacgagaa gcgagctttc cgtcgcccac cgaagccgcc acagcagcgc cgccgcaacc
     2221 gcgagtgcgg cgcccatgat cccgacggcg actgcgatcg ccgtctcggc ggttactcgc
     2281 acgaagtgct cggaagcgta ttgcgccagt gcggtggaga cgttctcgac cacgggaccg
     2341 tagcgttcgg cggcgccggc gggcatcagc accagcatgt cgccgtcgac gatgttgtga
     2401 ccggtcagcg acacgtccga cgccaacacc gacataccag cagcgcgggc gaattcataa
     2461 gtggcagtgg gtaattccgg cgccccgcga ccgcgcagca ccttgttgat gacggcgacg
     2521 gtcggatcga cgagcgtgct gagaggtgcg gcagccggca acaccaggtc gatctggtgg
     2581 gagtcaccgg ccaggaccga cacccggcac gacggaggta cggccggcgg tgcggccgca
     2641 tgcgccggcg gcgcacggaa cgtggtcatc ggtgttgtct cttcttcgcg ccagcgctca
     2701 tcggcgttcc cgcggcgcgt cggtggtggc gacgaagtgc tcggcgattg ccgcggcgat
     2761 ctcgaacagc ttgcgccggg tcttcggaga catctcggtc ccgacgtcga tcaccccgcc
     2821 cggccgcaga tgcgtgtcga acggcacttc gatcaccacc tggccgcggc tggcgaactg
     2881 ctgctgaagg atgttgcggg tgcgcttgtc ggcatgtccg tccgagtcgt tgagcaccac
     2941 cacggtccgg tgcagcagcc cggtgaggcc gcggttggcc aaccactcca tggtctgccc
     3001 ggccgccgcg gcgccgtcca cccacggcga cgacaccacg atcagcgcgt cgagatcccg
     3061 cagcacttcc tgggtgaccg ggctgtccat ggtcgagccg cagtcgatga tcgagatcgt
     3121 gaaatggcga tccagccgtg ccgcggcctc gcggtagatc gccgggtcga gcacccggcg
     3181 gcgcgcggtc gacgcctcgc cggcgaggac gaacaagccc gacgaattgt tgccgacgcg
     3241 actgcgcacg tctgcgaacg tgtcgaggtg atcgtcggcc gccagctccc aatacgaacc
     3301 tgcggcgtgc gggtcgacgc ggctgcccag cttgccgaac gatgtgtcgg catcgatggc
     3361 gaccacacgg tcgtcctggc gtacttcggc gaggatcgaa ccgacaccgg ctgcgatcgt
     3421 cgtcttgccc acgccgccct tgccgagcac gccgatcttg tagtggccgc gcagcaccga
     3481 acggatcttg gcttccagcg cggcctggcg cagctcgtcg ggcgactgtc cgagattgac
     3541 cgttccgaac gtcgccttgt agaccgcgcg ccgccagccg cgtcccggcg gcggtttgcg
     3601 ggtgggcacc agctcgctgg tgcggatgct gtcgacgtac gaccaattgg tcggcggcgt
     3661 cggcggctcc tgccagcgtg gtgtgggctg ccacccacct ccgggccgcg gaccggggcc
     3721 ggcctgctca cgagctggtg gccatgtcga cggccgatcc gggcggcccg gattgaacat
     3781 ccgcatggac tcagatgagg gcggcgggcc cgatggcggc gcgggctgct gatgttgcgc
     3841 ctgctggccc ggcggccctg gctgccggct tggcggcggg tagttcgccg gcgcagtccg
     3901 atagtgcatt cctgttgtcg gaccgcccgg ctcaggcgtc tgcggccact gctgctgagg
     3961 cgcgccttcc tgcgggcggg cgggggcccg ggtctgcggc gtgggccccg cccggctctg
     4021 tgggctgcag aggagcctgc tctgtgggct ggatggggac ttcctctgcg gcgtgggcgg
     4081 gcggctgtgc ccccacctcc tccggcggcg gcccgagatg ctcctgcagg aagtcgtcgc
     4141 gttctgtcac agctggctcg atctccttct aagggttgtc attgggcttg cccagcagcg
     4201 cggcctcggc ggggcccgac acggtgtcgt gctgcgtcag cgcgtccgct cgggtgagcg
     4261 ccggaccgac ggcgaacaca cgcatcagtg gccacggcgc ttgccgcgcc gaaccgggcg
     4321 tgatccccaa tgatcgcagc gacgcatcgt cgagcgaaat gccgtaccga accccttgat
     4381 cgctgatcca ccacagcgac tcgcgactgg tggcctcagg cgaagcgctg gtggtcatca
     4441 cgaggttggt cgcacctggc gccatgtata cctgatcggc ctcgatggtc gccggtccgc
     4501 gggcatcctt gacgagctgc agcagccgcg agtcagagcc gatcggaatg ggcaggccct
     4561 tgccggacag cactgtggtg actgctgagc ggtccgtcga acctttcgcc caggccacac
     4621 acgtgaccgg gttcgcccca gtgtcgacga acgtcaggcg cgtggtgggg tagtagccga
     4681 ccggcagtgg gttgacgacg ggcacgtggg tcagcgtgtc cggggccact gcgagcggca
     4741 gcggattgcc gaaggagttg gccgagcgca gcagcgttgc cacgaacggc gagatccgct
     4801 gtacgccatc ggtcagcagc acgtagaagt tgtcgtcgcc ggtttccaga tcccgcaccg
     4861 acagcaccga tccgaccacc gcgccgggtg ccaggttcca gccgccgggt gcgccggcgt
     4921 tgggcacggc tggcgtggtc agtggctcgg tggcgggcag cgcgtcgaac agcgcgttgg
     4981 agatcgtgac cggtcgcggc gcggcggagt ccacccccaa cgccagcgca accgccttgt
     5041 tcgacaggtc gacctgcgac cgctggtcgt cccacacgac gtacgtcgcg ccgttatgcg
     5101 tgacgagcac ggccagtggc ggacgcaact gctgcgcgcg ctcaccgatc gtcagcggcc
     5161 cgccgatcgc tgtgacgacg gaagaggcag acaccttgcc gggcgaggcg gtgtcgcaga
     5221 tcgcccactg cgacgcgtcc cccgtgcgca gaggcatcgc catcggcgcg ccggcgatcc
     5281 ctaccgtcgg gccctgaggg tacttcgcca gctcagaggc tttgacgaac gtggggttgg
     5341 ccgcctgacc ggcgatcagt cgagcgctga ccaggttcag cgcgggatgc agtcggccgt
     5401 cgaccagcac gaacaccgca ccggtgtccc ggtcggcgag gatcgtcgac tcgccgacgt
     5461 ggccggccgg cttgaaccac gacagcagga acatcagcgc cacgccgagc agggcgaaga
     5521 tgaagcccag gagaagcgac gcgcgctggc gctgcaccgg gtcgaactcc atccgcacgc
     5581 cgtggtgcga cagcgccgcg gcgtttcggc gctgccagaa gaagtgcgcc gacacctgca
     5641 ggccgctcgc caggttcaac gccatgaatc agtgccttat cctcgcccgc gtaaccgata
     5701 accggaggtc aagcgctatc cggccgcccg gggaacggaa agcagccgag ccaggtcctg
     5761 gcgcagcgcg cccggggtgg tgggccagac gctggtccag cccgacccgt ctgggcccgt
     5821 cgtgtaggtg acgctgacgc tgacgcggcc gtgttcggtg tcgatgaccg tcaacaccgc
     5881 cggatgcaca taccgggtcg cgccgtggtc gacgaccgtg accaccgcca tcgcggactc
     5941 gttcatccgg gtgccggcga cgagcacggc gacctgatcg ggtgacaggc ccagacgtgc
     6001 cagcgcggtg ccgacggccc accggtcctg gccgtgcgtc gccgtcagag cggactgcag
     6061 cgccttcgtc ggcacattca cgccgtcgat cgcggccggc tcggcgtctc cgaccgcgtg
     6121 cagcaccgcg tcgcagatca gctcgatctg ctgatcggcc gccgcgctct cgccgaccgg
     6181 cgcaagaacc agctcgtcgc cgttgcgagc gatcatcgcc agccaccgac tgcgccggca
     6241 caccgacagc gtccgctcct ggaccgctcc gtcggagtcc ggcacagggc ggcgaatgac
     6301 gacgacgacc tcgcgctcag gccggccaac cacggccatc cagtcacgga cggcgtcgtc
     6361 gacgcggccg tcgggatcga tgacaccggc cgcgaccagg ctggcgtact cggcggtctg
     6421 ggtcagcggc ctttcaccga ccatcgtcgc gacggtcggt ccggagtcga tggacgggat
     6481 gaacgggcgc agccgcagcg cgatcggcat ggtttcaacg ccgaggagcg cctgcagcac
     6541 ccagatgcca gccggggagg tagtgagatc agccattgct ttggttgacg aaaagacgca
     6601 aaagcacccg atgcgaggtg ccgcaggggc gctcttgcgt ctacttcgcg gagtttgccg
     6661 gcgggtcagc cgaacgagtt ggccgccgat atgtcggtgg cgatcatgtc gtcgttggca
     6721 ttgctgacga cctgggagtg ccgcatgatg acctccttgc cggcgtcgat gccggagttg
     6781 atcatcatct gcgcctgctc gaacgcgccg ccgccgcggg agtcgaacac ggagcgcaca
     6841 tgggccagca cgttctgcgc ctgactgcgg acatcctcga gctgtgcgct ggcggtcgag
     6901 atcgactgca catagtcggg cagcattgcg ggattgtaaa cgaacattcc gtccatttat
     6961 gtgaaagcct ttctgactaa accgaattag ggacgccggc gatggtcgat tgcgacgact
     7021 gttcctggtg ctcgtagttg tcggcggctt gccgcagcgt gttgatcagc tcgccccact
     7081 gcgtggtgag cttcatctgt gcgttgtgaa tttcctcggt cgtggtgatg ttcgtgctcg
     7141 ccgccaaccc gaaccagccc tgcgaggagt gcacgtcgtg ggcggcgttg aggtagttct
     7201 gcagggtggc gttggcctgg tcgcgcagct gctcgagctg ctgtgcggcg ctgcgcatct
     7261 gatcgggagt gacgagtaac tgcgaatccg ccatctgtgt ggttccttct ggtttgtttg
     7321 ctgatgaact gagtgtaggc cgcgcgcaac ggcggtctgc gcaccaattt tctggcttac
     7381 tcccccctcc cggacgccgg cacggtggtc aactgtatgg tccgcgctgg tttggcgttc
     7441 tcttggctcc ggtcgtctcg catcatcgcc ggcgcgccat agaggccgcc ggtgccgccg
     7501 gtcatcgcgg gctgcgccga cgtcgccacc tcgggaacag tggcggccgg gctggcggac
     7561 cagcctgtcg gcagcttcgg cggtccgggc gcgttgaagc tgctggtggg ccgcgtgaag
     7621 ctggacatca ccgcgctgcc gggcaacccg gcaccgccgg cgccacgggc cgcggcgtcg
     7681 gtgcccgctg cgatcggacc cc
//
LOCUS       DASWBF010000256        16084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_418629,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000256 DASWBF010000000
VERSION     DASWBF010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16084
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418629"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VF874_09255"
     CDS             complement(<1..199)
                     /locus_tag="VF874_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176257.1"
                     /translation="MVDNDGSSATSRRRHFVRLMLFAGFLLGLFYLVAVARVIDVEAA
                     RRAVAETGPAAPVIYVVASAVL"
     gene            375..1349
                     /locus_tag="VF874_09260"
     CDS             375..1349
                     /locus_tag="VF874_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176258.1"
                     /translation="MRDKDWQLYYSHWAGCRMLDALVGGPELALRYAQSLRPCAKDEW
                     VDPLWADGGAVVDLDRRRLLFFGDELMVEMPERRALMSVLAAVWPDYAICWAYDGTAE
                     LAGYVGAELPPHTWDKQPKLRLALDRNALCHLVSVVDAEGQLRMWPLWWHLSKAWHGP
                     ALLDKLPGRGVRTLTLGKIPEGGVHIDVPRKTLGAWQTADTMGFFQALPDLWGGWQTE
                     CWEDRFEEQVIRCNGALRVPQLDLAAGVDSAQAWIRNRVFQSFADSPAGQILKLAELL
                     APVRPGLVVSDDAVADHGVRPSKAEWARFVDACDGLRDAHASERTVAS"
     gene            1372..1599
                     /locus_tag="VF874_09265"
     CDS             1372..1599
                     /locus_tag="VF874_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_007409270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEX9176259.1"
                     /translation="MRTTIDLPDDLHKQALAIARDTHRTLSETVADLIRRGLGAASTD
                     AISIDPGTGLPLVSVGTVVTPEEVRSLEDEE"
     gene            1596..1777
                     /locus_tag="VF874_09270"
                     /pseudo
     CDS             1596..1777
                     /locus_tag="VF874_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1908..2246
                     /locus_tag="VF874_09275"
                     /pseudo
     CDS             1908..2246
                     /locus_tag="VF874_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
     gene            complement(2634..3097)
                     /locus_tag="VF874_09280"
                     /pseudo
     CDS             complement(2634..3097)
                     /locus_tag="VF874_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
     gene            3234..4109
                     /locus_tag="VF874_09285"
     CDS             3234..4109
                     /locus_tag="VF874_09285"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9176260.1"
                     /translation="MSGFHDGELAVQRQAGVEAAAARLSSMLSRAELRDGTAAFLHDA
                     TFAAITARDPAGQLWISPLSGPPGFLNRAGPRTLTIRACFPDQAPLHELLVGQPIGLV
                     VVEFAAKRRVRVNGTLTDVHNGELVVDVEQAYGNCPQYIRQRHLTTESVGAGQARVSR
                     RRTLAAEDVSLIQSADTFFLGTTHPERGNDASHRGGPLGFVRTDGETLWWPDYPGNNM
                     FNSFGNLAADPTAALLFVDFRTGHTLHLSGTATVQWNIPGDPDDDTRTGRGVHFTPTR
                     VAAGRLLPAREIGRD"
     gene            4149..4583
                     /locus_tag="VF874_09290"
     CDS             4149..4583
                     /locus_tag="VF874_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9176261.1"
                     /translation="MPDEEMNSERDFNERNIDEFRRNGGKVGGQFEGFPLLLLTSTGA
                     RSGAQRVNPVAYFDIDGKIYVIASSAGRDRDPVWAHNIRANPTASVEIGSDAPRTVTA
                     HELPRDERDRIYEIVVQRAPGFGDYQTQTDRIIPVFELTPND"
     gene            complement(4580..5998)
                     /locus_tag="VF874_09295"
     CDS             complement(4580..5998)
                     /locus_tag="VF874_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX9176262.1"
                     /translation="MTRLDRFFEISARDSTIGTELRGGLVTFIAMAYIIVLNPIVLSG
                     SADVAGHKLGFTQVSAVTSLAAGAMTILFGVVARLPFAFAAGLGINSFLASSVVGTLT
                     WPEAMGLVVIDGLIIVAVAATGLRKLIFDAVPMPLKLAITAGIGLFILFIGLIDAGFV
                     GSTKLPSPPVGLGTNGLGSINTVPTVVFVFTLLVTGVLVARKMRGAILIGLVAGTVVA
                     VVVEAIWHLGPATKNHGGWSLSVPTLSGSPFALPDLSLVGAFSLNSFGRIGILAAVML
                     VFTLVFTNFFDAMGSFTGLSRQAGLADEQGNFPRLQSALVVEGVGAVAGGAVSASSNT
                     VFIESGAGIGEGARTGMASLVTGGLFLAAMFLTPLAAIVPSEVAAAALVIVGTMMASV
                     LREIDFSEFSVALPVVLTVAVMPLSYSIANGIGVGFIAWVVLRAASGKAREISPLLWI
                     VAAGFLLYFARGWIDTLIGSRG"
     gene            6136..7107
                     /locus_tag="VF874_09300"
     CDS             6136..7107
                     /locus_tag="VF874_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176263.1"
                     /translation="MAEVLILAVIVMGVVAAVGSGGSQIARAAAPRKQTHPRLSRLEV
                     ISLSVLALTALVVAIGLANRVGDAWWLLPVVTWFVIYLAVRAWSRERALRRLDAERQK
                     RQQEAARDRQRLEEFGREGVSLLERANDAIKKIVSTEAARTGWLGEPAELDFRAELAT
                     IEETLRKATAIQKVVAEASTIPNPTKDDVQLVKDAKRTLKQLDGSVREQVNLLSKCSD
                     EAIELDRMLRDDREQSRIALQRGEVRARLSALIYGAELTSAGAPSDAVDAVSARVDAF
                     RELRALMARDQPDDRIAAETEKDSSAGQTPSNGFRQSVDKVFRHIRR"
     gene            7214..7918
                     /locus_tag="VF874_09305"
     CDS             7214..7918
                     /locus_tag="VF874_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX9176264.1"
                     /translation="MAIHEQLLEAVDGRRGVEAADRLCEACVVLLDVDAAAISLVFDG
                     ASSGTLGSSGAPARAYDELQFTLGEGPCLDSVAHRLPMLVVDLTEEPRWPAYGPALLA
                     HQIRSVCAMPVVVAGEFVGALDLFRAQPGELEGDALAGAIAAAELAGIAVLDLLDADL
                     QAAASDPSSDAWAELRALSRAEVSQATGMLVAQLGVEPPEALMRLRAHSYATGRNATD
                     VARDILDRRLTLERDE"
     gene            7933..8682
                     /locus_tag="VF874_09310"
     CDS             7933..8682
                     /locus_tag="VF874_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX9176265.1"
                     /translation="MTDIPRETRVLDAVVTLVDTLLHDFDVVDLLTDLTQQCADLLDV
                     ASAGFLLADPLRKLRLLAATSEQTRDLELFQLQADEGPCVDCYTSGQPVSVADLQNAT
                     ERWPRFVPAALDAGFASVHAIPMRAAGIVLGALGLFGTRPGELNDADLLVGQSLTHIA
                     CVAILQEIPPTPSIVMPQLRQALTSRVVVEQAKGFLRESLGVSIEEAFHLLRTYSRNH
                     QRHLTDVARRLMTEPGARPEVLSAMSELAHG"
     gene            complement(8673..9473)
                     /locus_tag="VF874_09315"
     CDS             complement(8673..9473)
                     /locus_tag="VF874_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011896039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX9176266.1"
                     /translation="MADYDAQPGRTAPDLSPAQRDADEAELYAGLRGVAGIVAGAQKV
                     IDLLRDVAEFAVQAIPGVDGAGVALVDTRDGAPRIQTWAATAVFVSNIDTVQYDDLRE
                     GPCLTCMQSRRPVVSGSLGSDSRWPHFGGRVARMGVHSALSLPLVVGDEVIGAINSYA
                     NSRDAFGEHAVRLGSQFAGPAAVSVYNAQLLANAQERTRALQRALDSRAVIDQAIGII
                     RSRSGLSAAEAFERLIQMSQSENVKLYAVAERLVEEAVRRVRARQRQP"
     gene            complement(9514..11148)
                     /locus_tag="VF874_09320"
     CDS             complement(9514..11148)
                     /locus_tag="VF874_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX9176267.1"
                     /translation="MSKFTETMYANAHRSRKGMVTGEPDAPVRHTWGEVHERARRVAG
                     GLAASGIGHGDAVAVLAGAPVEIAPTAQGIWMRGACLTMLHQPTPRTDLLRWAEETMA
                     VIKMIDAKAVVISDPFMAAAPVLAELGMRVLTIEDLLDSPPAEPVDTDDDDVAFMQLT
                     SGSTGSPKAVQITHRNFVANAEAMFAGAQVDADTDVIVCWLPLFHDMGMTGFLTVPMY
                     FGVELVKVTPMDFLRDTLLWAKLIDKYKGTMTAAPNFAYNLFAKRLRRQAVPGQFDLS
                     SLRWALSGAEQVEPADLEDLIDAGRPFGLRPEAILPAYGMAETTVAVSFTECGSGLAV
                     DEVDADLLAVLHRAVPATRGNTRRLAMLGPVLEGLEARIIDEDGGVLPARGVGVIQVR
                     GGCVTRGYTTVAGFVAAQDEQGWYDTGDLGYLTETGSIVVCGRVKDVIIMAGRNIYPT
                     DIERAAARVPGVRPGCAVAVRLDAGHSRETFAVAVESAAWQDRKEVRRIERRVAHEVV
                     SEVDMRPRNVVVLEPGMIPKTPSGKLRRAHALALVN"
     gene            complement(11527..11913)
                     /locus_tag="VF874_09325"
     CDS             complement(11527..11913)
                     /locus_tag="VF874_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family toxin-antitoxin system"
                     /protein_id="HEX9176268.1"
                     /translation="MHDYKVEVSRDGRWWMIHVPAIDQLTQARRISEIEDMARSLIAV
                     STDTPLTDIAVHITSIDVPEVGDIAARAREIEAARRAAVHVNEAAQRAAADYAQALAK
                     AGIPVRDAATLLHLSPQRISQLAKAR"
     gene            complement(11913..12113)
                     /locus_tag="VF874_09330"
     CDS             complement(11913..12113)
                     /locus_tag="VF874_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9176269.1"
                     /translation="MVKEEPTRSVIKRLKEAGFTRISTDGRHSKWTHPSGVWVPVPDS
                     HRTISPGVVRKINKAIDESRRK"
     gene            complement(12198..13913)
                     /gene="recD"
                     /locus_tag="VF874_09335"
     CDS             complement(12198..13913)
                     /gene="recD"
                     /locus_tag="VF874_09335"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEX9176270.1"
                     /translation="MTAAEWQLAVNATGLLQTFNAAGVIESSDTHVAQRLTQLAGEDD
                     ERAALAVALVVRALRGGSVCVDLRSVEKQVGAADLPWPEPSSWVTAVQASPLLGSPPM
                     LRLYGDLLYLDRYWREEQQVADDLLALLPASPTEAVPDITRLFPTGYEEQRGAAEIAL
                     SQGLTVLTGGPGTGKTTTVARLLALFAEQAELAGKAGLRIALAAPTGKAAARLQEAVQ
                     LEVDRLDPVDRQRLTGLRATTLHRLLGSRPDTSSRFRHHRGNRLPHEVIVVDETSMVS
                     LTMMARLLEAVRQGTRLLLVGDPDQLASVEAGAVLADLVDGLSGRSDARIAALKTSHR
                     FGESIGKLAEAIRDGDANKAVEVLRDGGEHIEWVDADNPAEDLRKVVLPHALRLRQAA
                     ILGDDASALAILDEHRLLCAHRRGPYGVQHWNRQVERWLTEATGEPIWSAWYPGRPVL
                     VTANDYGLGLYNGDTGVTVRHDDVLRAVIAGAAEPHAFATSRLADVDTMHAMTIHKSQ
                     GSQADEVTVLLPPEESRVLTRELLYTAVTRAKSKVRLIGSEAQLRGAIARPAIRATGL
                     RRRLQ"
     gene            complement(13910..>16084)
                     /locus_tag="VF874_09340"
     CDS             complement(13910..>16084)
                     /locus_tag="VF874_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9176271.1"
                     /translation="RLRVVRRETLGRSGFANLPIDELREHISADLAADIGALLAGGVT
                     YDGHPLQARHIAVIVETHKDARCCHRALADAGIQSVYTGDSDVFDSDAADDWLSLLEA
                     FDQPQRPGVVRAAAATMFFGETAETLAAGGDALTDRVAETLREWAGHARERGVAAIFE
                     AAQLNGMSDRVLSWQGGERHMTDLAHMTQLLQETAHREHYRLPALRDWLREQREERSG
                     ASERSRRIDSDAAAVQIMTVWVAKGLQYPIVYLPFAFNRRAPDRDLVLFHDDGVRCLH
                     IGGKDSPDFKVVEKLGRKEAASDDSRLTYVAMTRAQSQVVAWWSPAWDEPNGGLSRLL
                     RGRRPGEHVVPDRCEPAKISDDDAMARLREWEAAGGPVIEDSVVCSVAPVPVEPPPSD
                     LDARHFHRGIDTAWRRTSYSGLIRAAETTGVSSEPEVTELDDEAGDIPVVRSAAGADI
                     PSPMADLPTGAKFGTLVHAVLETADPFAADLAAELESQIREHSAWWPVDVPAAELAAA
                     MVPMHDTPLGPLANGLTLRQIGLPDRLRELDFEFPLAGGDLREATPNIRLADVGHLLR
                     DHLLADDPLAPYADRLISDALGGQSLRGYLSGSVDAVLRIAGHYLVVDYKTNWLGDGD
                     RPLTAADYDRPRLAEAMLHSDYPLQALLYSVVLHRFLRWRQPGYEPDRHLGGVLYLFV
                     RGMCGPATPIVDGHPAGVFSWQPPATLVTALSDLLDAGRAAA"
BASE COUNT         2733 a         5592 c         5127 g         2632 t
ORIGIN      
        1 cgagcacggc tgacgccacg acgtagatca ccggtgccgc cggacccgtc tccgcgaccg
       61 cgcgccgcgc ggcctcgacg tcgatgactc gcgcgacggc aaccaaatag aacagcccga
      121 gtaggaaccc ggcgaacagc atgagtcgca cgaagtggcg tcgtcgggat gtcgctgagg
      181 aaccgtcgtt atcgaccatg ctgacggtga ttgtcccgca ccaagcaggt ggcgccgaat
      241 cggatgacca ccaggaatgc aggcggctcg gcgtgtcaga ccctcggtgg aaactcgggt
      301 tagtcgacgc ggcatcggag accgcgtcaa tgggaattgg gggcgacatg gggaacaggg
      361 cgaacttcgt catcgtgaga gataaggatt ggcagctgta ctactcacac tgggcgggct
      421 gccgcatgct cgatgcgctg gtcgggggac cggaactcgc gttgcgatac gcgcagtcgc
      481 tccggccgtg cgccaaggat gagtgggtcg acccgctgtg ggctgacggc ggcgcggtcg
      541 tggatctcga ccgtcgccgg ttgttgttct tcggcgacga actgatggtg gagatgccgg
      601 aacgccgggc cctgatgagt gtgctcgccg cggtatggcc cgactatgcg atctgctggg
      661 cgtacgacgg aaccgctgaa ctggcgggat atgtcggggc cgagttgccg ccccacacgt
      721 gggacaagca gccgaagctg cggctcgccc ttgaccgaaa tgcgttgtgc catctggtct
      781 ctgttgtcga cgccgaggga caacttcgga tgtggccact gtggtggcac ctcagtaagg
      841 catggcacgg gcccgcgctg ctggacaagc tgcccggccg tggtgttcga acgttgacac
      901 tcggcaagat ccccgaaggc ggcgttcata tcgacgtgcc ccgaaagacg ttgggagcgt
      961 ggcaaaccgc cgacacgatg ggcttcttcc aagcccttcc tgacctttgg ggcgggtggc
     1021 agacggagtg ctgggaggat cgcttcgagg aacaggtgat ccgatgcaac ggagctctac
     1081 gggttcccca acttgacttg gctgccggtg tcgatagcgc tcaggcgtgg atccgcaaca
     1141 gggtgtttca gagcttcgcg gacagcccgg cagggcagat cctcaaactc gctgagctgc
     1201 tcgcgcccgt cagacccggt ctcgtcgtca gcgacgatgc ggtggccgac catggagtcc
     1261 gtccaagcaa ggctgagtgg gctcgctttg ttgacgcgtg cgatgggctg cgggacgccc
     1321 atgccagtga acgcaccgtc gcatcatgat gcagtaatgc gaggattaag cgtgcgcacc
     1381 accattgatc tcccggacga cctgcacaaa caggcgctgg caattgcgcg cgacacccac
     1441 cgcacattga gcgaaaccgt cgccgacctc atccgacgtg gcctaggagc cgccagcacc
     1501 gacgcgatct ccatcgatcc cgggacggga ctgcccctgg tgagcgtcgg gacagtcgtc
     1561 acgcccgagg aggtgcgctc gttggaggat gaggagtgac cgcgccgctc gacgcgaacg
     1621 tgctcaccgc gctggtcgtc gccgagcatg tgcatcacga cgccgccgcg gaattgctcg
     1681 ctcgcgcgaa gccgcatacg gacagttggc gactctcgat agcggcccgg cgcaactgca
     1741 caacgacgtc gcggtactca tttccacggc gcggtgacgt cgaacgtttc agccacagac
     1801 ggcgggcgga ccctcaattc gaccgcatag cgcagtcata gctgcccctg ccaccgtgga
     1861 gatcatgcaa gacccacacg aacacgacct cggactggcc catgatcttc gactcgctcg
     1921 aagcggccgt cgccggcgag aacgcccgat tgaccccaca aatcgccccg cccgaacagg
     1981 catgcaccgc ggtgtacgga tacgacaccg gatatgcggc cagcgtcaag aacatgtcac
     2041 gggtgacact gagctctgac aacgttttcg gtgacggcgg ggacgccgaa ctcgccagtg
     2101 tcacaggcga tccggccagc ggcatgacca tcgcgctcgt catcggcgtc ggcgagaaat
     2161 cctccagcac gccaccgccc gaggttccag cgagcggcgg tccgccaccg aacgggcgcc
     2221 cgcccagccc gcctccagtg cgctgaggta tcccgtcacc gcgagacgat ctgtaatctt
     2281 cactgtctca acgcttttct tcgcgtggat gtcttgtgcg ccgggtgcgg cgacgtttgt
     2341 cgctgcgagg cgggcagttc gactattgcc ctcgtcggtg gcatatgggg ccgaagtgac
     2401 gattgttacc acacgagcat caggcaccac attttcccct tcgattaggt ggtggtgttc
     2461 ttgcccacct ctagcgtcaa ggcgagcttt ggcctcgtca agttcagcct tcgccgcttc
     2521 gaacgcgtcc gctgctacac gaatatgtcg caagtgccgc gcatcgcgtg ctggcgcggt
     2581 gtgcggatcg aggtgctcca gccagttctc ggtcttcgtg agttccttgt ggctcaacgc
     2641 ctttcgtcgc ttggataccc gcgacggacg gtgcgatcga gaatgccgag cgtggcccgc
     2701 agagcggttt ccgagatgac cggaaatatg ccggcctcac gccatgtcgg gccgatgtct
     2761 gaaaccagtc gtagaaagac gtgacgattg tgcccgtgcc gtcggaggtc ggctcgagcc
     2821 ggtatccgta cacgtgaccg atctgcgggc ggatctggcc caacactgtc caggagatca
     2881 gccggttttt ctcgaattca cggatcgaga cggtcacgtc gtactttccc agctcgggga
     2941 agtcattcag cgactcgcga tccatgtgga cgacgaagct gtcgccggcg gcggtgactg
     3001 agtcgccctc gacatggccc tgcgggtcgc acaacacggc gaagatgccg gcggcgggcg
     3061 ccgcgatggt tcgttggacc tcgattcgat cagtcacgga ccgatcctgt cacggtgcgc
     3121 cgcgtcgacc gcacggcagc catccggact atccccacca tgtggtgcgt tgagaagggc
     3181 atgatcgcag agtcgacaac gagcctggac atggacgcga ggccgagccc gacatgagcg
     3241 gattccacga cggcgaactg gccgtgcaga gacaggccgg cgtcgaggcg gcggccgccc
     3301 gactcagctc gatgctctcc cgtgccgaac tgcgtgacgg cacggccgcc ttccttcacg
     3361 atgcgacgtt cgcggcgatc accgcgcgtg accctgcggg acagctgtgg atctccccgc
     3421 tgagcgggcc gccgggcttc ctgaaccgcg cagggccgag gacgctcacc atccgagcgt
     3481 gcttccccga ccaagccccg ctgcacgagc tgctcgtcgg tcagccgatc ggcctggtcg
     3541 tcgtcgagtt cgcggccaaa cgccgggtgc gcgtgaacgg cacgctcacc gacgtgcaca
     3601 acggcgagct ggtcgtggat gtcgagcagg cctacggcaa ctgcccgcaa tatatccgac
     3661 aacgacactt gacaaccgag tcggtcggtg ccgggcaggc ccgagtaagc cgacgcagaa
     3721 cccttgcggc ggaggatgtt tcgctgatcc agtcagcgga caccttcttt ctcggcacca
     3781 cccaccccga gcgcggcaac gacgcttcgc accgcggcgg cccgctcgga ttcgtgcgca
     3841 cagacggcga aaccctctgg tggcccgact atccgggcaa caacatgttc aacagcttcg
     3901 gcaacctggc tgccgacccg acggccgcct tgctgttcgt cgacttccgt accggacata
     3961 cgctgcacct gtccggcacc gcgactgtgc agtggaacat cccgggtgat cccgacgacg
     4021 acacccgcac gggacgcggt gtgcacttca cacccacgcg cgtggccgcc gggcggctcc
     4081 tccccgcacg cgagatcggc cgggactgac atcccacgta gccgcaatcg ccgtgccacg
     4141 atgagaccat gcccgacgaa gagatgaatt ccgagcgtga cttcaacgag cgcaacatcg
     4201 acgagttccg ccgcaacggc ggcaaggtgg gcggtcagtt cgaaggcttc ccgttgctgc
     4261 tgttgacatc caccggtgcc cgcagcggcg cccagcgcgt caacccggtg gcctacttcg
     4321 acatcgacgg caaaatctac gtcatcgcct catcggccgg tcgcgaccga gatcccgtgt
     4381 gggcccacaa cattcgcgcc aacccgacgg cgtcagtcga gatcggctcc gacgcgccgc
     4441 ggacggtcac cgcacacgag ttgccgcgcg atgagcgcga ccgcatctac gagatcgtcg
     4501 tgcaacgcgc ccccgggttc ggcgactacc agacgcaaac cgaccggatc atccccgtct
     4561 tcgagctgac accgaacgac taacccctag atccgatgag ggtgtcgatc cacccgcgcg
     4621 cgaagtacag caggaagccc gccgcgacga tccacaacag cgggctgatc tcgcgggcct
     4681 taccggatgc ggcgcgcagc accacccagg cgatgaaccc gacgccgatg ccgttggcga
     4741 tcgagtagct cagcggcatc accgccaccg tcagcacgac aggcagcgcc acggagaact
     4801 ccgagaaatc gatctcccgc aacactgacg ccatcatcgt gcccacgatg acgagtgcgg
     4861 ccgcagccac ctcggacggc acgattgcgg ccagcggcgt caaaaacatt gccgccaaga
     4921 acaatccacc cgtcaccagg ctggccattc cggtgcgtgc accctcaccg ataccggcac
     4981 ccgactcgat gaacaccgtg ttcgaggacg ccgacaccgc gccaccggcc accgcgccga
     5041 caccttccac cacgagcgcg gactgcaggc gcggaaagtt gccctgctcg tcagcgagcc
     5101 cggcctggcg tgacaggccc gtgaagctgc ccatcgcgtc gaagaagttg gtgaacacga
     5161 gggtgaacac cagcatgacg gccgccagga tgccgatgcg cccgaagctg ttcaggctga
     5221 atgcgccgac cagcgacagg tcgggcagag cgaacggtga acccgacagc gtcggcaccg
     5281 acaggctcca cccgccgtgg ttcttggtcg cgggtccgag atgccagatg gcctcgacga
     5341 cgacggcgac caccgttccg gcgaccagcc caatgaggat ggcgccgcgc atctttcgcg
     5401 cgacgaggac accggtcacc aacagcgtga acacgaaaac gaccgtcggc acggtgttga
     5461 tcgagccgag gccgttggtc ccgagaccca ccggcgggga cggcagcttc gtcgaaccga
     5521 cgaatccggc gtcgatgaga ccgatgaaca agatgaacag cccgataccg gcggtgatcg
     5581 cgagcttcag cggcatcggc acggcgtcga agatgagctt gcgcagcccg gttgccgcca
     5641 cggcgacgat gatcaggccg tcgatgacga ccaggcccat cgcctcgggc cacgtcagcg
     5701 tgccgacgac ggagctggcc agaaacgagt tgatgcccaa tcccgcggcg aacgcgaacg
     5761 gcagccgcgc gacgacgccg aacaggatcg tcatcgcgcc cgccgccagc gacgtcaccg
     5821 cggacacttg cgtgaacccg agtttgtggc cggcgacgtc ggcgctgccc gacagaacga
     5881 tcgggttcag cacgatgatg taggccatcg caatgaacgt gaccaggccg ccacgcaatt
     5941 cggtgccgat cgtcgagtcg cgcgcggaga tctcgaagaa tcggtcgagg cgagtcacgg
     6001 gtttgaccct aaggcgcgcg gagtgtgcgc actcgcgctg accgcacgtc ggtgcgcact
     6061 cctactatgg cgaataggtt gcgcccgtgt cagagaggtg cggctgggag gtccagatat
     6121 ccgatggtgc ggttgttggc agaggttctg atcctggcgg tgatcgtcat gggggtcgtc
     6181 gctgccgtcg gtagcggcgg atcccaaatc gccagagccg cggcgccgcg caagcagacg
     6241 catccccgat tatcccggct cgaggtcatc agcctctcgg tgctcgccct gaccgcgctt
     6301 gtcgtcgcta tcggtctcgc taacagggtc ggagatgcat ggtggctgct gccggtcgtg
     6361 acgtggttcg tcatctacct cgccgtccga gcgtggtcta gggaacgggc gctgcgccga
     6421 ttggatgctg agcggcaaaa acgccaacaa gaagctgcgc gggaccgcca gcggcttgag
     6481 gagtttggtc gagagggagt cagccttctc gaacgcgcga acgacgcgat caagaaaatc
     6541 gtgtcgaccg aggccgcacg caccgggtgg ctgggcgagc ccgccgagct ggattttcgg
     6601 gctgaactcg ccacgatcga ggaaaccctg aggaaagcaa ccgcaatcca gaaggtggtg
     6661 gctgaggcat cgactattcc caatcccacg aaggacgacg ttcagctggt caaggacgcg
     6721 aagcgcacct tgaagcagct agacggttct gttcgagaac aggtgaatct tctttcgaaa
     6781 tgtagcgacg aggcaatcga actcgaccga atgctgcgag acgaccgcga acaatcaagg
     6841 atcgctctgc aacgcggtga ggtccgggca cgcctgagtg cattgatcta cggcgctgag
     6901 ctcacgtcgg ccggtgcccc ttccgacgca gtcgacgccg tcagtgcgcg cgtcgacgcg
     6961 ttccgtgagt tgagggcctt gatggcgagg gatcagccag acgatcgcat cgcggcggaa
     7021 accgaaaagg attcgtcggc gggacaaacc ccgtccaatg gctttcggca atcagtcgac
     7081 aaggtgtttc gccatatccg ccggtagcgt gaggttccga tgtcgtaccc actctcgtaa
     7141 atccagcccg actggtggag gctttagaca tgacgccacc tcgccacgtc gatccccttc
     7201 tgttcggcta aatgtggcga tccacgagca gctcctcgag gccgtcgacg gccggcgtgg
     7261 ggtagaggcc gccgaccgtc tctgcgaggc ctgcgtggtt cttctcgacg tcgacgccgc
     7321 cgccatctct ctcgtcttcg acggcgccag cagcggcacc ctcggctcca gcggtgcccc
     7381 cgcccgcgcc tacgacgaac tgcagttcac gctcggcgag ggaccctgcc tggattcggt
     7441 cgcccaccgg ctcccgatgc tcgtcgtcga cctcaccgag gagcctcggt ggccggccta
     7501 cggcccggcc ttgctcgccc accagatccg cagcgtctgc gcgatgcctg tcgtcgtagc
     7561 cggcgagttc gtcggcgccc tcgacctctt ccgcgctcag ccgggcgagc tggagggtga
     7621 cgccctggcc ggcgccatcg ccgccgccga gctggccggc atcgccgttc tcgacctcct
     7681 cgacgccgac ctgcaggccg ccgcctccga cccgagcagc gacgcctggg ccgaactccg
     7741 cgccctgagc cgcgccgagg tcagccaggc caccggcatg ctcgtcgccc agctgggggt
     7801 cgagccgccc gaagcgctga tgcgcctgcg cgcccactcc tacgccaccg gccgcaacgc
     7861 caccgacgtc gcccgcgaca tcctcgaccg ccgcctcacc ctcgaacgag acgaataacc
     7921 atggagggca acatgaccga catccctcgc gaaacccgcg tgctcgacgc cgtcgtcacg
     7981 ctcgtcgaca cgctgctcca cgacttcgac gtcgtcgacc tgctgaccga cctcacccag
     8041 cagtgcgccg acctcctcga cgtcgcctcc gccggatttc tgctcgccga cccgctgcgc
     8101 aagctccggc tcttggccgc cacctccgag cagactcgcg atcttgaact tttccagctc
     8161 caagcagacg aaggtccgtg cgtcgactgc tacaccagcg gccagcccgt ctccgtcgcc
     8221 gacctgcaga acgcaactga acggtggcct cgcttcgttc ccgccgcgct ggacgctggc
     8281 ttcgcctccg tgcacgccat cccgatgcgc gccgcgggca tcgtgctcgg tgccctcggc
     8341 ctgttcggca cccgccccgg tgagctcaac gacgcggatc tgcttgtcgg acaatcgctt
     8401 acgcatatcg cctgtgtggc gattcttcag gagatcccgc ccacccccag catcgtcatg
     8461 ccgcaactcc gtcaagcgct caccagccgc gtcgtcgtcg agcaggccaa gggctttctc
     8521 cgcgaaagcc tcggcgtgtc gatcgaggag gcgttccacc tcctgcggac ctactcacgc
     8581 aatcaccaaa gacacctcac cgacgtcgcc cgccgcttga tgaccgagcc gggtgcccgc
     8641 cccgaggttc tttcggcgat gtccgaactg gctcacggct gacgctgccg cgcccgtacc
     8701 cgccgcaccg cctcctcgac cagccgctcc gccaccgcgt acagcttcac gttctcggac
     8761 tggctcatct ggatcaaccg ctcgaaggcc tccgctgcgc tgaggcccga ccggctgcgg
     8821 atgatcccga tcgcctggtc gatcaccgcc cggctgtcca gcgctcgctg cagcgcccgc
     8881 gtccgctcct gcgcgttggc cagcagctgc gcgttgtaca ccgacaccgc cgcgggcccg
     8941 gcgaactgcg accccaaccg caccgcatgc tcgccgaacg cgtcccggct gttggcgtac
     9001 gagttgatcg cgccgatcac ctcgtcgccg accaccagcg gcagcgacag cgcggagtgc
     9061 acccccatcc gggccacccg gccaccgaag tgcggccacc ggctgtcact gcccagcgac
     9121 ccgctgacca caggtcgcct cgactgcatg cacgtcaggc acggcccttc cctcaggtcg
     9181 tcgtactgca cggtgtcgat gttgctgacg aaaaccgctg tggcagccca tgtttggatc
     9241 cgaggcgcgc catcccgggt gtcgaccagc gcaacgccag cgccgtcgac gccggggatc
     9301 gcttgcaccg cgaactccgc cacatcgcgg agcagatcga tcaccttctg cgcgccggcc
     9361 acgatcccgg ccaccccgcg cagcccggca tacagttcgg cctcgtccgc atcgcgttgg
     9421 gccggggaca ggtcgggcgc tgtgcgaccg ggctgggcgt cgtagtcggc catcgtgggc
     9481 aacaaatcta ctcggtgccg cgctccgaca aggtcagttg acgagcgcca atgcgtgggc
     9541 ccggcgcagc tttcccgacg gcgtcttcgg gatcattccc ggttcgagca ccaccacgtt
     9601 gcgcggccgc atgtcgacct cgctgaccac ttcgtgcgcg acccgacgct cgatgcgacg
     9661 cacctctttt cggtcctgcc acgcggcgga ctcgacggcg acggcgaacg tctctcgtga
     9721 gtgcccggcg tccaggcgca ccgccaccgc gcaccccggc cgtactccgg ggacccgggc
     9781 cgcagcgcgt tcgatgtcag tcgggtagat gttgcggccg gccatgatga tgacgtcctt
     9841 gaccctgccg cacaccacga tgctgcccgt ctcggtgaga tagccgaggt cgccggtgtc
     9901 gtaccagccc tgctcatcct gagccgcgac gaaaccggcc accgtggtgt agccccgcgt
     9961 cacacatccc ccgcgcacct gaatgacgcc gacgccccga gccggcagca cgccgccgtc
    10021 ttcgtcgatg atgcgcgcct ccaggccttc cagcaccggg cccagcatgg ccagccgccg
    10081 ggtattgccc cgtgtcgcgg gaacagctcg gtgcagcacg gcgagcaggt cggcgtcgac
    10141 ttcgtcgacg gccaggccgc tgccgcactc ggtgaacgac accgccacgg tcgtctcggc
    10201 catcccgtag gccggcagga tggcctccgg ccgcaaaccg aacggcctgc ccgcgtcgat
    10261 caggtcctcc aagtccgccg gctccacctg ctcggcgccc gacagcgccc accgcagcga
    10321 cgacaggtcg aactggcccg gcaccgcctg ccggcgcagc cgcttggcga acaggttgta
    10381 ggcgaagttc ggcgccgccg tcatggtgcc cttgtacttg tcgatcagct tggcccacag
    10441 cagcgtgtcg cgcaggaagt ccatcggcgt gacctttacc agctccaccc cgaaatacat
    10501 tggcaccgtc aggaatccgg tcatgcccat gtcgtggaac agcggcagcc aacacacgat
    10561 cacatcggtg tccgcatcga cctgggcgcc ggcgaacatc gcttccgcgt tggctacgaa
    10621 gttgcggtgg gtgatttgca cggccttggg cgacccggtc gagcccgacg tcaactgcat
    10681 gaaagccacg tcgtcgtcgt cggtgtccac cggctcggcc ggcgggctgt ctagcaggtc
    10741 ctcgatggtc agaacccgca tccccaactc ggcgagcact ggtgccgcgg ccatgaacgg
    10801 atccgagatg accaccgcct tcgcgtcgat catcttgatg acggccatgg tttcctcggc
    10861 ccaacgcagc aagtccgtgc ggggcgtcgg ctggtgcagc atggtcaggc aggcaccacg
    10921 catccagatg ccctgcgcgg tcggggcgat ctcgaccggg gcaccggcca gcaccgcgac
    10981 ggcgtccccg tgcccgatgc cggaagcggc cagcccgccg gcaacgcgac gggcgcgctc
    11041 gtgcacctcg ccccacgtat ggcggaccgg tgcgtccggc tcgccggtaa ccatgccctt
    11101 gcgacttcta tgggcgttgg catacatcgt ctcggtgaat ttgctcatga cgattccttc
    11161 ggcgtcgcca ccacggcgtc gagttgggag ctccgcgcat aaacacgaac cccgtttgat
    11221 ccgtcacccc gggcggagtg cggcgtggag cttgtcggcg accccgccga cgaagcaccg
    11281 agtggacgca ctgactgtac agacccgacc gcagccgggc agcgcagcgc gccgatatcg
    11341 atgaccgcgg acattgcagg cggactcccc cgagagcaca accgtggaag tgtcggctta
    11401 cgtcgggggg gtcaatgccc gttcggcggc gacatcagat cgccgccgtg acgtttccgt
    11461 aaggctgggt ggtcaacctt ccaggtcgga cgttgctcgg cggtatggca actgctccta
    11521 agtcggttat cgcgccttgg cgagttgact aatgcgctga ggtgaaaggt gcagaagcgt
    11581 agctgcgtcg cggacgggga tgccggcctt cgccaaggct tgagcgtaat cagcagcggc
    11641 gcgttgtgcc gcctcgttga cgtggaccgc ggcgcggcgg gctgcttcga tctcacgggc
    11701 gcgcgcagcg atatcaccga cctcagggac gtcgatgctg gtgatgtgca cagcgatgtc
    11761 agtgagtggt gtgtcggtac tgacggcaat gaggctgcgg gccatatcct caatctcgct
    11821 gatgcggcgc gcctgggtga gttgatctat cgctgggacg tggatcatcc accagcgacc
    11881 atcgcggctg acttcgacct tgtagtcgtg catcacttcc gcctgctctc gtcgatggct
    11941 ttgttgatct tgcggaccac accagggctg atcgtgcggt ggctgtccgg aacgggtacc
    12001 cacacaccgg acgggtgcgt ccatttggag tgccggccat ccgtgctgat acgcgtgaaa
    12061 ccggcctcct tcaaccgttt gataactgac cttgtgggtt cttccttcac cacaacgata
    12121 gtctagtgcg ctagacgatt tctagtcaag cgcactagac aataaattct cgaggtcagg
    12181 gcaccttgat ccgccgtcta ttgcagccgg cgccgcagcc ccgtcgcgcg gatcgctggg
    12241 cgggcaatgg cgccgcgcag ctgggcctcg gacccgatca ggcgaacctt ggatttggcc
    12301 cgcgtcaccg cggtgtagag cagctcacgg gtcagcaccc tcgactcttc tggcggcagc
    12361 agcaccgtga cctcgtcggc ttggctgccc tgactcttgt ggatcgtcat cgcgtgcatg
    12421 gtgtcgacgt cggcgagccg gctggtggcg aatgcgtgcg gctcggcggc accggcgatg
    12481 acggcccgca gcacatcgtc gtggcggacg gtcacgccgg tgtcgccgtt gtagagaccg
    12541 agtccgtaat cgttggccgt caccagcacc gggcggcccg ggtaccaggc cgaccagatc
    12601 ggctcgccgg tcgcttcggt cagccaacgt tcgacttggc ggttccagtg ctgcacgcca
    12661 tacggtcctc gtcggtgtgc gcacaacagc cgatgctcgt cgagtatggc cagcgcggac
    12721 gcgtcgtcgc cgaggatcgc tgcttgtcgc aggcgcagcg cgtgggggag aaccactttc
    12781 cgaaggtctt ccgccgggtt gtctgcgtcg acccattcga tgtgctcgcc gccgtcccgc
    12841 aggacctcga cggccttgtt cgcgtcgccg tcacggatcg cttctgccag tttgccgatc
    12901 gactcgccga accggtgcga cgtcttcaac gcggcgattc gcgcgtcgct gcgtccgctc
    12961 agcccgtcga ccagatcggc cagcaccgcg ccggcctcca ccgacgccag ctgatcggga
    13021 tcgccgacca gcagcagccg cgtgccctgg cgcaccgctt ccagcagccg cgccatcatc
    13081 gtcagcgaca ccatcgaggt ctcgtcgacc acgatgacct cgtgcggcag ccggttgccg
    13141 cggtggtggc ggaaccgcga cgacgtgtcc gggcggctgc ccagcagccg gtgcagcgtg
    13201 gtggcccgca gcccggtcaa ccgctgacgg tcgaccgggt cgaggcggtc cacttcaagc
    13261 tgcaccgcct cctgcagtct ggccgccgcc ttgccggtcg gtgccgccag cgcgatgcgc
    13321 agccccgcct tgcccgccaa ctccgcctgt tccgcgaaca gcgcgagcag acgcgcgacc
    13381 gtcgtcgtct tacccgtccc gggcccgccg gtgagtactg tcagcccttg cgacagtgcg
    13441 atttccgcgg cccctcgctg ttcctcgtat ccggtgggga acaaccgggt gatgtctgga
    13501 accgcctcgg ttggggacgc cggcagcaga gccagcagat cgtcggccac ctgctgttcc
    13561 tcgcgccaat accggtcgag gtacaacaga tcgccgtaca gccgcaacat cggtggcgag
    13621 ccgagcaagg gactggcctg cacggcagtt acccacgacg acggttccgg ccagggtagg
    13681 tccgcagcgc cgacttgctt ttcgaccgag cgcaggtcga cgcacaccga gccgccgcgc
    13741 agcgcgcgca ccaccagcgc gacggccagc gcggcccgct cgtcgtcctc gccagcgagt
    13801 tgtgtaagtc gttgcgccac atgagtatcc gatgattcaa tgacgccggc ggcgttgaac
    13861 gtctgcagca gaccggtggc gttgacggca agctgccatt cggcggcggt catgccgcgg
    13921 ccctcccggc gtcgagcaga tcggacaacg cggtgacgag cgtcgccggc ggttgccaac
    13981 tgaacacccc cgccggatgc ccgtcgacta tcggcgtcgc cggtccgcac atgcctcgta
    14041 cgaacaggta cagcactccg cccaagtgac ggtcgggctc ataacctggc tgccgccatc
    14101 gcaagaaccg gtgcagcaca acgctataca acagcgcctg cagcggatag tcggagtgca
    14161 gcatcgcctc ggccagtcgc ggccggtcgt agtccgcggc ggtcaacggt cgatccccgt
    14221 cgccgagcca gttcgtcttg taatcgacga cgaggtagtg gcctgcgatg cgcaacacgg
    14281 cgtcgaccga accgctgagg tatccgcgaa gtgactggcc gccaagcgca tcgctgatca
    14341 gccggtcggc atacggtgcc agcgggtcat cggcgagcag gtggtcgcgg agcagatggc
    14401 cgacgtctgc gagccggata ttcggcgtgg cctcgcgcag atcgccaccc gccagcggga
    14461 attcgaagtc caactcccgc agccggtccg gcagcccgat ctgccgcaat gtcaacccgt
    14521 tggccagcgg acccaacggt gtgtcgtgca tcggcaccat cgcggcggcc aattcggctg
    14581 cgggcacgtc gacgggccac cacgccgagt gctcccggat ctgcgactcc aactcggcag
    14641 cgagatccgc ggcgaacggg tccgccgtct ccaataccgc gtgcaccagc gtgccgaatt
    14701 tcgcgcccgt cggcagatcc gccatcgggg aagggatgtc ggcgccagca gccgaacgaa
    14761 cgactgggat gtcgccggcc tcgtcgtcca actcggtgac ctccggctcg ctgctcaccc
    14821 cggtcgtctc cgcggcccgg atcaagccgg aatacgacgt gcgccgccac gccgtgtcga
    14881 ttccgcggtg gaagtggcga gcgtcgaggt ccgacggcgg cggctcgacc ggcacgggcg
    14941 ctaccgagca caccaccgaa tcttcgatca ccggcccgcc cgcggcctcc cactcacgca
    15001 gccgcgccat cgcgtcgtcg tcggaaatct tcgcgggctc acagcgatcg ggcaccacat
    15061 gctcgccggg ccggcgtccg cgcagcagcc gcgacagccc gccgttcggc tcgtcccacg
    15121 ccggcgacca ccacgccacc acctgcgact gcgcccgggt catcgccacg tacgtcaacc
    15181 ggctgtcgtc actggcggcc tccttgcggc ccagcttctc gaccaccttg aaatcagggc
    15241 tgtccttgcc gccgatgtgc aggcaccgca cgccgtcgtc gtggaacagc accaggtcgc
    15301 gatccggggc tctgcggttg aacgcgaacg gcagatacac gatcgggtac tgcagcccct
    15361 tggcgaccca caccgtcatg atctgcactg cggcggcgtc gctgtcgatg cgtcggctgc
    15421 gttcggatgc cccgctccgt tcttcgcgtt gctcacgcag ccaatcccgc agcgcgggca
    15481 agcggtaatg ctcgcggtgc gccgtctcct gcagcagctg cgtcatgtgc gcgaggtcgg
    15541 tcatgtgccg ctcgccgcct tgccacgaca gcacgcggtc gctcatgccg ttgagctgcg
    15601 cggcttcgaa gatcgccgcg actccgcgct cgcgcgcgtg ccctgcccac tcgcgcaacg
    15661 tctccgcgac gcgatcggtc agcgcgtcgc cgcccgccgc aagggtttcc gcggtctcgc
    15721 cgaagaacat cgtcgccgcg gcggcgcgca ccacgccggg gcgctgcggc tgatcgaacg
    15781 cctccagcag cgacaaccag tcgtcggcgg cgtcggagtc gaacacgtcg gagtcgccgg
    15841 tgtacacgga ctgaatcccg gcgtcggcca gcgcgcggtg acagcagcga gcgtccttgt
    15901 gcgtctcgac gatcaccgcg atgtggcgcg cctgtagtgg atgtccgtcg taggtcaccc
    15961 cgccggcgag cagcgcaccg atgtcggcgg cgaggtcggc gctgatgtgt tcgcgtagtt
    16021 cgtcgatcgg taggttcgca aagccgctgc ggcccagtgt ttctcgtcgc accacccgca
    16081 gccg
//
LOCUS       DASWBF010000257         9724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_225992,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000257 DASWBF010000000
VERSION     DASWBF010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225992"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VF874_09345"
     CDS             complement(<1..331)
                     /locus_tag="VF874_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEX9176272.1"
                     /translation="MAPGRPPEKLDSLATSFSRSLRVEGKADRTLVLYGQSIDYFSAW
                     LVERGLPADLSSLTRHNVLGWIESLRGKRQSDGTIRTRWRGLRRFVNWLLAERIIKKD
                     PLAGITVD"
     gene            complement(377..1423)
                     /gene="ligD"
                     /locus_tag="VF874_09350"
     CDS             complement(377..1423)
                     /gene="ligD"
                     /locus_tag="VF874_09350"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX9176273.1"
                     /translation="MATAAEELDVDGVKVRLTNPDKVYFPKLGSDGTKRTLVDYYLTV
                     SKGPMLTALRDRPTHLQRFPDGIDGEEIYQKRVPQHHPDYLETCRITFPSGRTADALK
                     ITHPSAIVWAAQMGTVTLHPWQVRCPDTEHPDELRIDLDPQPGTGFKEASTIAVDVLK
                     PLLDELGLVGFPKTSGGRGVHVFLRIKTDWDFIAVRRAGIALAREIERRAPDLVTTSW
                     WKEERGKRLFIDFNQNARDRTFASAYSVRRTPIATVSTPLTWDELATADPDDYTMASV
                     PDLVARRADPWADIDEKKQSIEPLLEMVRADEERGLGDMPYPPNYPKMPGEPKRVQPS
                     RNTDLTSRDTHKQV"
     gene            complement(1423..2475)
                     /locus_tag="VF874_09355"
     CDS             complement(1423..2475)
                     /locus_tag="VF874_09355"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9176274.1"
                     /translation="MDDVDLPVLPPIEPMLAKAQAKVPDEQSVWSYEPKWDGFRALVF
                     RDGDDVMLQSRNGRELGRYFPEVLDALRNETADRCVLDGEVVVPREIGGRIRLDWESL
                     SQRIHPAQSRVKMLAEQTPAHFIGFDALATGETSLMGEPFRTRRHALTELITEKQWCH
                     VTRTTDDPDQGARWLTTFEGAGLDGVIAKRLDGPYLPGKREMVKVKHARDADCVAIGY
                     RIHKSGEGVGSILLGLYRDGELQMVGGAASFTAKDRLKLLADLEPLRKADDLVYDGEP
                     SRWNSAADKRWIPVRPAKVAEVAYDQMEGNSVHGRRFRHAVRFVRWRPDRDPRSCTFD
                     QLDVPLNYDLYDVLES"
     gene            2543..3619
                     /locus_tag="VF874_09360"
     CDS             2543..3619
                     /locus_tag="VF874_09360"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9176275.1"
                     /translation="MELPVMPPVSPMLAKPVRGIPPGASYEPKWDGFRTICFRDYDEV
                     ELGSRNERPMTRYFPELVAAVIAELPQRCVIDGEIVIATDHGLDFETLQQRIHPAESR
                     VRMLAEQTPASFIAFDLLALGDDDYTGRPFSERRAVLVGALAGCGPSIHVTPATTDLA
                     TAQQWFEEYEGAGLDGVIAKPLTITYQPDKRVMFKIKHERTADCVVAGYRVHKSSDEA
                     IGSLLLGLYKDDGSLASVGVIGAFPMAERRRLFAELQPLVTSFDNHPWNWAAHQAGER
                     TPRKNEGSRWNAGKDLSFVPLRPERVVEVRYDHMEGERFRHTAQFNRWRPDRDPRSCT
                     YAQLEHPLTFSLGDVVAGLGQTGK"
     gene            3701..3832
                     /locus_tag="VF874_09365"
     CDS             3701..3832
                     /locus_tag="VF874_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176276.1"
                     /translation="MAAGAIMQGSRGGVDLSKDDIDCVKSHLAEYYKKMGDTAPWER"
     gene            3848..4720
                     /gene="gluQRS"
                     /locus_tag="VF874_09370"
     CDS             3848..4720
                     /gene="gluQRS"
                     /locus_tag="VF874_09370"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX9176277.1"
                     /translation="MSPAGRFAPSPSADLHIGNLRTAVLAWLFARSTGRRFLIRVDDL
                     DDRTRADIGTRQLADLAAIGLTWDEPPEWQTQHRHRYDDVIARLAERGLLYECYCSRR
                     DIQQAPRAPHAPQGAYPGTCRDLTDAERAVRWEATGRPPALRLRAEVTSFTVVDLLHG
                     DYAGVVDDFVVRRGDGVAAYNLAVVVDDAAAGVDQVVRGDDLLPSSPRQAYLAGVLDY
                     AQPAYAHVPLVLNHGGQRLAKRDGAVTLAEIGVQRAIAQMSASLGWPSEDLEQLLAQF
                     DPSKLPREPWIYSP"
     gene            complement(4860..6080)
                     /locus_tag="VF874_09375"
     CDS             complement(4860..6080)
                     /locus_tag="VF874_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9176278.1"
                     /translation="MRILLIGAGGVGSAFCAIAARRDFFDTVVVCDYDESRARAAAKA
                     VGDDRFSSARVDASSVQSVAQLVREHQITHVMNAVDPRFVMPIFDGALGGGADYLDMA
                     MSLSKPHPDEPYRLTGVKLGDEQFAMEDRWRAAGRLALVGIGVEPGLSDVFARYAQDH
                     LFDEIDELGTRDGSNLMVEGYDFAPSFSIWTTIEECLNPPVIWEADRGWFTTEPFSEP
                     EVFDFPGGIGPVECVNVEHEEVLLMPRWVKAKRATFKYGLGAEFIDVLKTLHKLGLDR
                     TEKVDVNGMKVSPRDVVAACLPHPATLGSSMRGNTCAGLWVTGVGKDGNPRSTYLYHV
                     VDNAWSMDEYGHQCVVWQTAINPVVALELLANGTWSGEGVLGPEAFDAVPFLDLLTAY
                     GSPWGQLELEAGGA"
     gene            complement(6178..7401)
                     /locus_tag="VF874_09380"
     CDS             complement(6178..7401)
                     /locus_tag="VF874_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9176279.1"
                     /translation="MTTLAESQSLLLQLLDPSTRANPYPVYRQFRERGPLQLRDANLT
                     VFSSFRDCDEVLRHPASSSDRLKSTIAQRAIAAGEEPRPFGKPGFLFLDPPDHTRLRK
                     LVSKAFAPKVVTALEPDITRLVTSLLDDVEAAATFDVVADLAYPLPVAVICRLLGVPI
                     EDEPKFSWASALLARALDPFTTFTGELPDNFAERMQAGLWLRGYLGDLIEKRRGQPGD
                     DLMSGLIAVEEAGDQLTEEEIIATCNLLLIAGHETTVNLIANAILAMLRHESQWAALA
                     NDSARASAVVEETLRYDPPVQLIGRIAGEDTTIGDVTVPKGDTMMLLLAAAQHDPDAN
                     ERPDEFDPDRETIRHLAFGHGPHFCLGAPLARLEASVALSAVTARFPDARIDSEPVYK
                     PNVTLRGLQTLAVTV"
     gene            complement(7406..8623)
                     /gene="tgt"
                     /locus_tag="VF874_09385"
     CDS             complement(7406..8623)
                     /gene="tgt"
                     /locus_tag="VF874_09385"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX9176280.1"
                     /translation="MAEQGSEFFTITASLSGRRGRCGVIRTPHGDIHTPAFIAVGTQA
                     TVKAVLPETMKALGAQAVLANAYHLYLQPGAEVVEQAGGLSAFMNWPGPTFTDSGGFQ
                     VLSLGAGFRKVLAMDTERVQADDVIAAGKERLAVVDDDGVTFRSHLDGSSHRFTPELS
                     MRIQHQLGADIIFAFDELTTLVNTRGYQERSVRRTHDWAVRCLAEHQRLSRPGRSPQA
                     LFGVVQGAQYEDLRRAAARGLRSLDFDGFGIGGALEKQNLATIIGWVTSELPEEKPRH
                     LLGISEPDDLFSAVAAGADTFDCVSPSRVARNAAVYSSTGRFNITGSRYRRDFTPIDP
                     ECDCYTCAHYTRAYLHHLHKSKEILAATLTTIHNLRFVIRLVDQIRESIYQGRFDDLR
                     EHVLGRYYSTRAE"
     gene            8689..9135
                     /locus_tag="VF874_09390"
     CDS             8689..9135
                     /locus_tag="VF874_09390"
                     /inference="COORDINATES: protein motif:HMM:NF017309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HEX9176281.1"
                     /translation="MQNRFVVVTGTAVCAVAVVAGCSSKPAESPQPPGSLPPATAQIT
                     VNGKSAGTTNDVHCTQDGWLHTIETGDDQSGVRVVLMTGDAISAQSVVINNVGDFNGS
                     VWPNNIGKADAAIIGTTFRVNGTAQGATTADPNQQTTASFDIKANC"
     gene            9194..9448
                     /locus_tag="VF874_09395"
                     /pseudo
     CDS             9194..9448
                     /locus_tag="VF874_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(9396..>9724)
                     /locus_tag="VF874_09400"
     CDS             complement(9396..>9724)
                     /locus_tag="VF874_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine peptidase"
                     /protein_id="HEX9176282.1"
                     /translation="RAIFRVWPNFPVHPQIDISGRTIKADAARRSFNARGGGITWAVV
                     DSGIDAGHPHFTGGNTLGAPDVKDLHRDFTHTLRPGEDTKDAALHDGYGHDPCGPDPT
                     SPNLPR"
BASE COUNT         1650 a         3223 c         3145 g         1706 t
ORIGIN      
        1 tatccacggt gatcccggcc aacgggtctt ttttgatgat gcgctcggcc agtagccagt
       61 tgacgaatcg gcgcagccca cgccaccgcg tacgaatggt gccgtcgctt tgcctcttgc
      121 cgcgtagtga ctcgatccag ccgagcacgt tgtgacgggt cagtgacgac aggtcagccg
      181 gtagcccacg ctcgacgagc caggcactga agtagtcgat gctctggccg tagagcacca
      241 gtgtgcggtc tgccttgcct tccacccgca aggaacggct gaagctggtg gccagcgagt
      301 cgagcttttc tggaggtctt cctggagcca tggatgccga ggttatcagg tgagcgccag
      361 tcatgctaac tctgggtcag acctgtttgt gtgtgtctct gctggtcaga tcggtatttc
      421 gactgggctg cacccgcttg ggttcgcccg gcatcttcgg gtagttcggc gggtacggca
      481 tgtcgccgag gcctcgctct tcgtcggccc tgaccatctc cagcagcggc tcgatcgact
      541 gcttcttctc gtcgatgtcg gcccacggat ccgctcgtcg cgcaaccaga tccggcacgc
      601 tagccatcgt gtagtcgtcg ggatcggcgg tggccagctc gtcccacgtc agtggtgtcg
      661 acaccgtcgc gatcggggtg cgccgcaccg agtacgccga cgcgaacgtg cgatcgcgcg
      721 cattctggtt gaagtcgatg aacagccgct tgccgcgctc ctctttccac cacgacgtcg
      781 tcaccaaatc tggtgcacgc ctctcgattt cacgagccaa cgcgatgccc gcccgtcgca
      841 ccgcgatgaa gtcccagtcc gtcttgatcc gcaggaacac atgcaccccg cggccaccgg
      901 atgtcttcgg aaagccgacc agccccagct cgtcgagaag cggtttcaac acgtcgaccg
      961 cgatcgtgct ggcctccttg aatcccgttc ccggctgggg gtccagatct attcgtagtt
     1021 cgtcgggatg ctcggtgtcc gggcagcgca cctgccacgg atgcagcgtg acggtgccca
     1081 tctgggctgc ccagacgatc gccgagggat gcgtgatctt cagggcgtcg gcggtgcggc
     1141 ccgacggaaa cgttatccgg cacgtctcca ggtagtcggg atggtgctgc ggcacccgct
     1201 tctggtagat ctcctcgccg tcgatgccgt cagggaatcg ctgaagatga gtcggccggt
     1261 cacgcagcgc agtcagcatc ggtccctttg agacggtcag gtagtagtcg accagcgtgc
     1321 gtttcgtgcc gtccgatccc agcttgggga agtacacctt gtccgggttg gtcagccgga
     1381 ccttcactcc atcgacatcg agttcctctg cggcggttgc catcacgact ccagcacgtc
     1441 gtacaggtcg tagttcagcg gcacgtcgag ctggtcgaac gtgcagcttc tcggatcgcg
     1501 gtccgggcgc cagcgcacga accgcactgc gtgccggaac cgtcggccgt ggacgctgtt
     1561 gccctccatc tggtcgtagg ccacctcggc gaccttcgcg ggccgcaccg ggatccagcg
     1621 cttgtcggct gcggaattcc atcggctggg ctcaccgtcg tacaccaaat cgtcagcctt
     1681 tcttagcggc tccagatcgg ccagcagctt cagccggtcc ttggcggtga aagacgctgc
     1741 gccaccgacc atttgaagct cgccgtcgcg gtagaggcct agcaggatcg agccgacacc
     1801 ctcgccgctc ttgtggatgc ggtatccgat tgcgacgcag tcggcgtcgc gggcgtgctt
     1861 gaccttcacc atctctcgct tgccgggcag atacggcccg tcgagtcgct tggcgatcac
     1921 cccgtccagg ccggcgcctt cgaacgtggt cagccagcga gcaccctggt cggggtcgtc
     1981 ggtggtgcgg gtgacgtggc accactgctt ttcggtgatc agctcggtca gcgcgtggcg
     2041 acgtgtgcgg aacggctcgc ccatcagcga ggtttcgccg gtggccagcg catcgaagcc
     2101 gatgaagtgc gcgggcgtct gctcggccag catcttcacg cggctctggg cgggatggat
     2161 gcgctggctt agcgactccc aatccagccg gatgcgtccg ccgatctcgc ggggaacgac
     2221 gacctcgccg tcgagcacgc atctgtcggc cgtctcgttc cgcagcgcgt cgagcacctc
     2281 cgggaaatac cggcccagct cccttccgtt gcgcgactgc agcatcacgt cgtcgccgtc
     2341 acgaaacacc agcgcgcgaa acccgtccca cttaggctcg taagaccaca cgctctgttc
     2401 gtcaggcacc ttcgcctggg ccttcgccaa catcggctcg atcggcggca gcacgggtag
     2461 gtccacgtca tccatcctgg ttcattgggt gataccgagc tagacctccg cacgagcggg
     2521 aagtcatcgc aacaatgagc cgatggagct ccccgtcatg ccgcccgttt cgccgatgct
     2581 ggccaaaccg gttcgcggga tcccgcctgg cgcgtcctac gagcccaagt gggatggatt
     2641 caggacgata tgttttcgcg attacgacga ggtggaactg ggcagccgca acgagcggcc
     2701 gatgacccgt tactttcctg aactggttgc agcggtcatc gctgagctgc cgcagcgctg
     2761 cgtgatcgac ggtgagatcg tcatcgccac cgaccacggc ctggatttcg agacgctgca
     2821 acagcgcatc catccggccg agtcgcgcgt gcggatgctc gccgagcaga cgccggcttc
     2881 ctttatcgcg ttcgacttgc tggccctcgg cgacgacgac tacaccgggc gaccgttcag
     2941 cgagcgccgc gctgttctcg tcggagcgct ggccggctgc ggcccgtcga tccacgtcac
     3001 ccccgcgacc accgacctgg cgacagcaca acaatggttc gaggagtacg agggagccgg
     3061 cctcgacggt gtcatcgcca agccgctgac gatcacctat cagccggaca aacgagtcat
     3121 gttcaagatc aagcatgagc ggaccgccga ctgcgtggtc gccggctacc gcgtgcacaa
     3181 gtccagcgac gaggcgatcg gctcgctgct gctcgggctc tacaaggacg acggcagtct
     3241 cgcatccgtc ggcgtcatcg gcgccttccc gatggccgaa cggcggcggc tgttcgctga
     3301 gctgcagccg ctggtaacca gcttcgataa ccacccgtgg aactgggccg cccaccaagc
     3361 cggcgaacgc acaccccgca agaacgaagg ttcacgctgg aacgccggca aagacctttc
     3421 gttcgtgccg ttgcgacctg agcgggtcgt tgaggtccgc tacgaccaca tggaaggcga
     3481 aaggttccgc cacacagccc agttcaaccg ctggcgcccg gaccgcgacc cccgctcgtg
     3541 cacttacgcg caacttgaac acccgctcac cttcagcctc ggcgacgtgg ttgctgggct
     3601 tgggcaaact ggcaaatagc atccaggacc gcacggggtc cggccacgca gcacggcgtt
     3661 tatcgcgtac aagctgctca aggccgtacc acgcggcgtg atggcggcgg gcgcgatcat
     3721 gcagggctct cgcggcggtg tcgacctgtc gaaggacgac atcgactgcg tgaagagcca
     3781 cctcgcggag tactacaaga agatgggcga caccgcgccg tgggagcgct gaactagatt
     3841 ccggcctatg agcccagcgg gtcgcttcgc cccgagtccg tcggccgacc tgcacatcgg
     3901 caacctgcgc accgcggtgc tggcatggct gttcgcccgg tcgacaggcc gccggttcct
     3961 gattcgggtg gacgatctcg acgaccgcac ccgagccgac atcggcacac gccaactcgc
     4021 cgacctcgcc gcgatcggat tgacctggga cgagccgccg gagtggcaga cgcagcaccg
     4081 gcaccgctac gacgacgtga tcgctcgcct cgccgagcgt gggctgctgt acgaatgtta
     4141 ttgcagcaga agggatatcc agcaggcgcc gcgagcaccg cacgcgccgc agggcgccta
     4201 cccgggcacc tgtcgcgacc tgaccgacgc cgagcgggcg gtccgttggg aagccacggg
     4261 ccggcccccg gcgctgcggt tgcgcgccga ggtcacctca ttcaccgtgg tggacctgct
     4321 gcacggcgac tacgccgggg tggtcgacga tttcgtggtg cgccgcggcg acggcgtggc
     4381 ggcgtacaac ctcgcggtgg tggtcgacga cgccgctgcc ggtgtcgacc aggtggtgcg
     4441 cggcgacgac ctgctgccgt cctcgccgcg acaggcctac ctcgccggcg tgctcgacta
     4501 tgcgcaacct gcgtacgccc acgtgccgct ggtgctcaac cacggcggtc agcggctggc
     4561 caagagagac ggtgccgtca cgctggccga gatcggggtc cagcgtgcga tcgcccagat
     4621 gtccgcgtcg cttggttggc cgtcagagga cctggaacag ctgctggcgc agttcgatcc
     4681 gtcgaagctg ccgcgggagc cgtggatcta ctccccttag cccgcgctga tgatcacact
     4741 ggacactttg gcaccaattt cccagacatc caccagtttt caaacgtgct atcgcatgcg
     4801 atagcgccat tcgagagaac accaatcacc gtcagcggtg actcaagtga atgatgtggt
     4861 tacgctccgc ccgcctcaag ctccagctgc ccccagggtg agccgtaggc cgtcagcaag
     4921 tccaggaacg gcaccgcgtc gaacgcctcc ggccccagca cgccttcgcc gctccaggtt
     4981 ccgttcgcca gaagctcaag tgcgacaacg ggattgatcg ccgtctgcca caccacacac
     5041 tggtgaccgt actcgtccat cgaccacgcg ttgtcgacga cgtgatacag gtaagtcgat
     5101 cgtgggttgc cgtccttgcc gacgccggtg acccacaagc cggcacatgt gttgccgcgc
     5161 atcgacgaac cgagcgtcgc cggatgcggc agacacgccg cgacaacgtc gcgcggactc
     5221 accttcatgc cgttgacgtc gaccttctcg gtgcggtcca acccgagctt gtgcagggtc
     5281 ttcagcacgt cgatgaactc cgcgcccagg ccgtacttga acgtcgcgcg cttggccttg
     5341 acccagcgcg gcatcaacag cacttcctcg tgctcgacgt tgacgcactc gaccgggccg
     5401 ataccgcccg gaaagtcgaa aacctctggc tcgctgaagg gttcggtggt gaaccagccg
     5461 cggtcggctt cccagataac cggcgggttg aggcattctt cgatcgtcgt ccagatcgag
     5521 aacgacggcg cgaagtcgta tccctcgacc atgaggttgg acccgtcgcg agtgccgagt
     5581 tcgtcgatct cgtcgaacag gtggtcctgc gcgtagcggg cgaacacgtc ggaaaggccc
     5641 ggctccacgc cgatcccaac caacgcgagc cgaccggctg cgcgccagcg atcctccatg
     5701 gcgaactgct cgtcgccgag tttgacgccg gtgagccggt atggctcgtc ggggtgaggc
     5761 ttcgacaggc tcatcgccat gtcgaggtag tccgcaccgc cgcccagggc gccgtcgaag
     5821 atcggcatca cgaaccgcgg gtccaccgcg ttcatcacat gcgtaatctg atgctcgcgc
     5881 accagttggg ccactgactg caccgaggag gcgtccaccc gagccgacga aaagcggtcg
     5941 tcgccaacgg ctttggcggc agccctcgcc ctgctctcgt cgtagtcgca gaccactacg
     6001 gtgtcgaaga agtcgcgtcg cgccgcgatc gcacagaacg ccgagccaac cccaccggcg
     6061 ccaatcagca gaatccgcat gggctagacc gtacgctgac cactccgcga gcatgcatgt
     6121 ttgtacgttg ctcaacggcg tgtctctggc acgacacgca cgctcgcggg aagggggcta
     6181 caccgtgacg gcgagggtct gcaggccgcg caatgtgacg ttcggcttgt acaccggctc
     6241 gctgtcgatc cgggcatccg ggaaccttgc cgtgacggct gacaacgcga ccgatgcctc
     6301 cagccgtgcc agcggcgcac ccaggcagaa gtggggccca tgcccaaacg ccaagtggcg
     6361 gatggtttct cggtccgggt cgaactcgtc gggccgctcg ttggcatccg ggtcgtgctg
     6421 cgcggcggcg agcagcagca tcatcgtgtc gcccttcggc accgtcacgt cgccgatcgt
     6481 cgtgtcttca ccggcgattc gtccgatcag ctgcaccggc gggtcgtagc gcagtgtctc
     6541 ctcgaccacc gccgacgccc gcgcggaatc gttcgcgagc gcggcccact gcgactcatg
     6601 gcgcagcatc gccaatatcg cgttggcgat caggttcacg gtggtctcgt gaccggcgat
     6661 cagcagcaag ttgcaggtcg cgatgatctc ttcctcggtg agctgatcgc cggcctcctc
     6721 caccgcgatc agcccggaca tcaggtcgtc gccgggctgg ccgcggcgct tctcaatcaa
     6781 gtcaccgagg tacccgcgca gccacagacc ggcctgcatg cgctcagcga agttgtccgg
     6841 caactccccg gtgaacgtgg tgaacgggtc gagggcccgg gccagcagcg ccgatgccca
     6901 gctgaacttc ggttcgtcct cgatcggcac gcccagcagc cggcagatca ccgccaccgg
     6961 caacgggtag gccaggtcgg cgaccacatc gaaagtggca gcggcctcga cgtcgtccag
     7021 cagcgacgtg accagcctgg tgatgtccgg ttcgagcgcc gtgaccactt tgggcgcgaa
     7081 cgccttgctg accagcttgc gcagccgagt gtgatccggc gggtcgagga acaagaagcc
     7141 gggcttgccg aacgggcgcg gctcctcgcc cgcagcgata gcccgttgcg cgatggtcga
     7201 tttgagtcgg tcgctggacg acgccggatg gcgcagcacc tcgtcgcagt cacggaacga
     7261 gctgaacacg gtgaggttcg cgtcgcgcag ttgcagcggg ccgcgctccc ggaactgccg
     7321 gtacaccgga tacgggttcg cgcgggttga cggatcgagc agctgcagca gcagagactg
     7381 tgactcggca agagtcgtca taccttcatt ctgcacgcgt cgagtagtaa cggccgagaa
     7441 cgtgctcgcg cagatcgtcg aaccggccct ggtagatgga ctcgcggatc tggtcgacca
     7501 gccggatcac gaaccgcagg ttgtggatcg tggtaagcgt cgcggcgagg atttccttgc
     7561 tcttgtgcag gtggtgcaga tacgcccggg tgtagtgcgc gcaggtgtag cagtcgcatt
     7621 cggggtcgat cggtgtgaaa tcacggcggt agcgcgaccc ggtgatgttg aaccgccccg
     7681 tcgaggagta caccgcggca ttgcgcgcga cccgggacgg ggacacgcag tcgaacgtgt
     7741 ctgctccggc ggcgacggcc gagaacaggt cgtcgggttc gctgatgccc agcaggtgcc
     7801 gcggcttctc ctccggcaat tcagacgtga cccagccgat gatcgtcgca aggttctgct
     7861 tctccagcgc gccgccgatc ccgaacccgt cgaaatccag tgacctcagc ccgcgcgccg
     7921 cggcccggcg cagatcctcg tactgagcgc cctggaccac cccgaagagc gcctgcggcg
     7981 accggccagg gcggctcaac cgctgatgct cggcgaggca gcgcaccgcc cagtcgtggg
     8041 tgcgccgcac cgagcgctcc tggtagccgc gggtgttgac gagcgtggtg agttcgtcga
     8101 acgcgaagat gatgtccgcg ccgagttggt gctggatgcg catcgacagc tccggcgtga
     8161 accggtgcga cgacccgtcc agatgcgagc ggaacgtcac cccgtcgtcg tcgacgaccg
     8221 ccagccgttc cttgccggcc gcgatcacgt cgtcggcctg cacccgctcg gtgtccatcg
     8281 cgagcacctt gcgaaaaccc gcgcccaggg acagcacctg gaacccgccg ctgtcggtga
     8341 acgtcgggcc gggccagttc atgaacgcac tcaagccccc ggcttgctcg acgacctcag
     8401 cgcccggctg taagtacagg tggtaggcat tcgccagaac agcttgtgca ccaagcgctt
     8461 tcatggtctc cggcagcacc gctttgacgg tcgcctgtgt gccgacggcg atgaacgccg
     8521 gggtgtggat gtcaccgtgc ggggtgcgga tcacgccgca gcgtccccga cggccggaca
     8581 gcgaagcggt gatcgtgaag aactcacttc cctgctcggc cacggaggta ttcaatcaat
     8641 ccgggggttt tgctttcggt tcggcacggc cgccgataga tttgccgagt gcagaaccga
     8701 ttcgtcgtcg taaccgggac ggccgtgtgc gctgtcgccg tcgtcgcggg ctgctcatcg
     8761 aagcctgccg aatctccgca gccgcccggt tcgctgccgc cggcgaccgc tcagatcacg
     8821 gtcaacggca agtccgccgg caccacgaac gacgtccact gcacgcagga cggctggctg
     8881 cacaccatcg agaccggcga cgaccagtcc ggtgtgcggg tcgtcctcat gaccggggat
     8941 gcgatcagcg ctcagtcggt ggtgatcaac aacgtgggcg acttcaacgg cagcgtgtgg
     9001 ccgaacaaca tcggcaaggc cgacgccgcc atcattggca ccacgttccg cgtcaacggc
     9061 accgctcagg gtgccaccac cgccgacccg aatcagcaga ccaccgccag cttcgacatc
     9121 aaggcgaact gctgagcggc agctagtggt gtgtcctgca ataaactggc tataggttgg
     9181 atatgatttc gggatgagac aacccgtgcc tgcgttggag atgtcgacag gacaacgtga
     9241 ggtcctggag tcgttggctc ggtcgcagtc gggtgcgcat cgggaggtgg tgcgggcgaa
     9301 ggcgctgctg atggcggctg atggtgtggc caacactgcg atcgcgcggt cgttgtcggt
     9361 gtcaccggcg tcggtgaaga gctggcgtga caggttcacc gaggaaggtt tggcgaagtt
     9421 gggtcaggtc cgcaagggtc gtggccgtaa ccatcgtgca gcgctgcgtc tttggtatct
     9481 tcgccgggcc gcagcgtgtg ggtgaagtca cggtgcagat ccttgacatc cggcgcgccg
     9541 agcgtattac cgccggtgaa gtgcggatgg ccggcgtcta tgccggaatc gacgaccgcc
     9601 caggtgattc cgccgccgcg ggcgttgaac gagcgccgtg ccgcgtcggc cttgatggtg
     9661 cggcctgaga tgtcaatttg cgggtgaacc ggaaaattgg gccagacgcg gaagatcgca
     9721 cgct
//
LOCUS       DASWBF010000258        26584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_418666,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000258 DASWBF010000000
VERSION     DASWBF010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 26584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26584
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418666"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..654)
                     /locus_tag="VF874_09405"
     CDS             complement(172..654)
                     /locus_tag="VF874_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176283.1"
                     /translation="MPTASPFARRADSSAYTAHGGATAVMRLYVHGSAVPVGRVAGGR
                     TVRDAHPQPGQRVRFDGVDEVEDAVQQCQRLRADEHDRVADRLDELNGVLRDLSSPVT
                     QAAGQAGEVFRADRLTEASEPDEVSERHRHVTRAWQCTGCPLGGVDRLGLERVAERHP
                     "
     gene            836..1231
                     /locus_tag="VF874_09410"
     CDS             836..1231
                     /locus_tag="VF874_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176284.1"
                     /translation="MTTTHNTRIRRIVRTAVFAGGLALAVSPFASLAVANAQYKESDF
                     AACLERDMPTDYCCEHAGGVMRNGACINPDDLMVPSSGVFEQTPTTPTTRPPVVATVP
                     TGVFEQVTTATPTPKPGSVVTAPITTATR"
     gene            1296..2690
                     /locus_tag="VF874_09415"
     CDS             1296..2690
                     /locus_tag="VF874_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX9176285.1"
                     /translation="MGGDSAATRSPFGASAFRGRRPTRSGDLAVETHGIAPIGEDQRY
                     GAPARLFTVWFAPQVNMTGVFTGTLAITLGLGFWLGLLAMVIGTVLGSFVVGYLSTWG
                     PRTGTAQLPISRMAFGGGVVLPATLQWLSSIAWDGLVGLFGADALAVLLGIPFWLAVL
                     IVLAVQGVVGFFGYEVIHRLQAVLTVVLFVTFVVFAVKLVGGHEIVTPAGVQGPDLLG
                     AFVLEVTVALSLAISWASYASDFSRYLPANSSQLKVFGFTFAGIVSAYVFVQGIGLAA
                     APALSEQTSEGVRSVMGGGVLGALALLVIALAAVGSAAMNDYSGSLALQTAGVRVRRP
                     VSALVVTVLAFALILWLHSGDMSQRFQDVLLLVSYWIPGFVAVTVIDWSIRARGRHTI
                     NPAAENTDRLDAVAALVTFVVAYGAAVPFMHTTLIEGPVARAWHGADTAYFVGFLVAA
                     LLYGGYRWRSMSRS"
     gene            complement(2674..3111)
                     /locus_tag="VF874_09420"
     CDS             complement(2674..3111)
                     /locus_tag="VF874_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9176286.1"
                     /translation="MSAPVEPIVVIPEDESWQLLASRTLGRLATSVGGQPEIFPVNFV
                     VQRRSVLFRTAEGTKLASAVANNRVAFEVDEHNFEGGWSIIVKGQAQMVRTQEEIAEA
                     ERAQLLPWIATRKRHFVRITPSEISGRRFRFGTEPDEDFNYAT"
     gene            complement(3115..3456)
                     /gene="fdxA"
                     /locus_tag="VF874_09425"
     CDS             complement(3115..3456)
                     /gene="fdxA"
                     /locus_tag="VF874_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9176287.1"
                     /translation="MTYVIGRECVDVMHRQCVQECPVDCIYEGERSLYINPNECVDCG
                     ACRLICEVGAIYYETDLPEDELQHLADNAAFFEETLPGRDEPLGSPGGAEGLGPVGVD
                     TPYVASLPRRN"
     gene            3968..5038
                     /locus_tag="VF874_09430"
     CDS             3968..5038
                     /locus_tag="VF874_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9176288.1"
                     /translation="MTSTELAVPKDWDEITPDWMTAALAARHPDATVEAVTVELRDDG
                     TNRRARLGLTYSAGTGPASVFVKAADPAHKDLIRLTSGMFHEPRLFQSGVELPLEHPT
                     VYAALIDEADYDFILVMEDLTTRGADPRDATRPMTVEQVATGVRGLGRMHGQFWGRRV
                     IEHPALGWLEPFVPWDGMQAAPLPEALSRLGDDAPAEVMSLTIEGLIEDTWKPFIRSL
                     TASPQTLLHGDPHIGNTYLLPSGEVGFLDWQVARRGNWSLDLGYFLQGALTIEDRRRS
                     ERQLLEEYRDALALPADELPSIDEIWLRYRASVAHGLTLWLCTASAGELWQRPDIAIA
                     LAQRYSAAYADLDARAAIAEIA"
     gene            5138..5293
                     /locus_tag="VF874_09435"
     CDS             5138..5293
                     /locus_tag="VF874_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176289.1"
                     /translation="MIKCDDGRAFPVAAQYNWDINGNGIASAFNDGSCGDEPGRIEAG
                     FTLTQVA"
     gene            complement(5398..5471)
                     /locus_tag="VF874_09440"
     tRNA            complement(5398..5471)
                     /locus_tag="VF874_09440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5435..5437),aa:Arg,seq:tct)
     gene            complement(5554..7044)
                     /locus_tag="VF874_09445"
     CDS             complement(5554..7044)
                     /locus_tag="VF874_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX9176290.1"
                     /translation="MSDKPNILVIWGDDIGLSNLSCYSDGVMGYRTRNIDRIADEGVR
                     FTDYYGEQSCTAGRAAFITGQNPYRTGLTKVGMPGATLGLQPEDPTIATALKAQGYAT
                     GQFGKNHLGDRDGHLPTAHGFDEFFGNLYHLNAEEEPELPDYPKDPEFRKKFGPRGVL
                     HSWANPDGTQRIEDTGPLTKKRMETCDEEFRDAAVDFIKRQHAAQTPFFVWFNSTHMH
                     FRTHTKPESIGRAGRWQSNYHDTMLDHDDTVGSLLDLLDELGIAEGTIVMYSTDNGPH
                     MNSWPDAGMTPFRNEKNSNWEGAYRVPALMRWPGHIPAGKVLNGIVSHNDWFVTLLAA
                     AGVPDIADKLLKGADLNGTTYKVHLDGHNQLPYITGEVDESPRQHFFYCSDDGDLTAL
                     RYDNWKFVFLEQRCPGTLQIWAEPYTELRFPKLFNLRTDPYEKADQTSNTYYDWVLDH
                     IFLFVPAQTYVAQMIQTLAEFPRRQESASFNLEQVIAKLKAGARSA"
     gene            7328..8005
                     /locus_tag="VF874_09450"
     CDS             7328..8005
                     /locus_tag="VF874_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176291.1"
                     /translation="MDDSQSEPQSRRLTTPRAAAVAGILFAAFFASSLVLLRSAIPDD
                     PFAQTAWVTRGETRITVALILAPLAGIAFLWFVGVLRDRFGVLEDRFFSTVFLGSGLL
                     FLAMVFVSMATAGAILTVSQTPTTPSNGLVYFGRSLMLQISNVWALRMAAVFMISLAT
                     IWIRTRLMPRWLAALTYLLAVTLLLVVSYSLWVTLVFPAWVGVVSLFIVIRDREQRRE
                     LGPPQDR"
     gene            8164..9642
                     /locus_tag="VF874_09455"
     CDS             8164..9642
                     /locus_tag="VF874_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9176292.1"
                     /translation="MVDAVDAAGLPEELGAFDMLLHRGEANPRTRSGIMALEILDTTP
                     DWNRFVAVFEHASRQVLRLRQKVVVPTIPTASPRWVVDPDFNLNFHVRRLRVPEPGTL
                     REVFDIAEVSLQSPLDISRPLWNVTLLEGLVDGRAALLLHMSHAVTDGVGAVEMFAHI
                     YDLERDPPAREPLPLPIPQDLTGNDLAREGLNRLPLTILGGVRGAVSGAAQVVGRVLT
                     NPVSAVEGVVEYARSGARVMSTVAEHSPLLRRRSLSSRSEAIDMKLTDIRAAAKAAEG
                     SINDAYLAALCGALRFYHQELGVPIDSLPMAVPVNLRAEADPAGGNRFAGVMISAPLG
                     LSDPKARIHNVRTQMCKRRDEPAIDMIGAVAPLIGLLPEPLVESIASSVVSSDVQASN
                     VPVYAGDTFIAGAKVLRQYGLGPLPGVAMMAVLISRGGMCTVTTRYDRASFTDDDLWA
                     RCLLTGFNDVLALGGPGRARPASFDEPAASIQPPSPNGSSPS"
     gene            9639..11297
                     /locus_tag="VF874_09460"
     CDS             9639..11297
                     /locus_tag="VF874_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase/lysophospholipid
                     acyltransferase family protein"
                     /protein_id="HEX9176293.1"
                     /translation="MSPSNNRSLDRRDMRLPGSVAEIEASAPGPKVGAFFDVDGTLVA
                     GFTGVIMTRDRFRRREISIGEFLGVVQAALNHQLGRADFEQLIYRGASMMKGRSLDDI
                     DELGERLFVQHIVSRIYPEMRELVRAHQVRGHTVALSSSALTVQVEPVARFLGIDNIL
                     SNRFEVDEHGVLTGGVLKPILWGPGKANAVQKFAADNDIDLKKSYFYADGDEDVALMY
                     LVGNPRPTNPKGKLAAVAAKRGWPVLRFSSRSGSSPTSALRTLAGIASMAPVAAGAVG
                     VGLLAGSKRRGVNFFTSNFGRALLAATGVNVNVIGEENLTAQRPAVFIFNHRNQVDPI
                     VAGYLVRDNFTSVGKKELESNPIAGALGKIMDAAFIDRDDPKAAVEGLHKVEELARKG
                     LSILIAPEGTRLDTTEVGPFKKGPFRIAMSVGIPIVPIVIRNAEVIAARDSSTFNPGT
                     VDVVVYPPIPVDGWTHDNLPERIAEVRQLFLDTLTNWPRDEVPETKLTSRATPKKAPA
                     KKAPAKKAAAKKAPAKKTAAKKTADDGNSQAAKKATPTKAKGRR"
     gene            11294..13642
                     /locus_tag="VF874_09465"
     CDS             11294..13642
                     /locus_tag="VF874_09465"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HEX9176294.1"
                     /translation="MSARTDDLDSFTATDDALVLAAVSSPAELELLNDWLAHQRREHP
                     ETKVEVLELPAEDDPAPGVVAQLAEQLAADEDRTVVPVRVFWVPGGLPTRVKLVGLLS
                     GRDAYRPPQILQRRILHRDASRARIVAGEPAKVSELRQQWQDTTVGHDPRDFARFVLR
                     RAALAIERVELRLLGPAYKSPRLVKPEMLASARFVEGLQRIPGATVEKAGQMLDELAT
                     GWSRFSVDLIPSLGRAIFSRGFDPNIDYDRAEIESMRQALETLPAVLLFSHRSYLDGV
                     IVPVAMQENRLPPVHSFAGINLSFGFMGPLMRRSGAIFIRRKIDDPLYKYVLKEFVGY
                     IVEKRFNLTWSIEGTRSRTGKMLPPKLGLLTFVADAYLDGRSDDILLQPVSISFDQLH
                     ETAEYAAYARGGEKTPEGLSWLYNFIKAQGERNYGKIYVRFPAAVSMRGYLGEPHGPS
                     VTDEAAKRLALQKMAFEVAWRILRATPVNATGLVSALLLTTRGRALTLDQLHHTLQDS
                     LDYLERKQTPMTNSALRLRTNEGVRAAVDALSHGHPVTRIDTGREPVWRIAPEDEHEA
                     AFYRNTLIDAFLETSIAELALAYAAHAEGDRVTAFWDRVTRLRDLLKFDFYFADSAAF
                     REHIAEEMAWHDGWEAQVRAGGHDIGELLHAKQPLMSPAMLRPFLEAYEIVADVLRDA
                     PAEIGEKDLTKLALGVGKQYSAQGRVRSNEAVSALLFATARQVAADQGLLQTAPDLAE
                     RRRAFLAELRSILRDMDMVELASLRQFTEREAQRGRERLEAG"
     gene            13939..15954
                     /locus_tag="VF874_09470"
     CDS             13939..15954
                     /locus_tag="VF874_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX9176295.1"
                     /translation="MTAAPTSTRRAAVWPVLTGVAVLAGVTATAIGAMSLAAALTATG
                     LPDPGSVTTYGLPFVRAAGEVAAVIAVGSFLFAAFFVPPQDNGVLDAAGYRAVRIGTV
                     ASGAWTVCAAVLVPLTISDVSGQPVFEHLNPLTIWSAAGLVNTAGAWRWTAFLAAMVT
                     IASLPVLRWSLTPFLFAGSLLTLMPLGLTGHSSAGGAHDVGTNSLVIHLVAGALWAGG
                     LLALLTHALRGAEHADLAARRFSKVAGWCFVAMAVSGVVNALVRITPGDLLDTTYGRL
                     VLAKVAALGALGVLGWRQRRSGLVVLRDNPTARGPLVRLALIEALVFGVTFGIAVGLG
                     RTPPPAPPIGYPSIPEVEIGYDFAGPPTLARVLFDWRFDLFFGTAAIVLAVVYLAGVR
                     RLRRRGDTWKAGRTFAWLCGCAVLLFATSSGVGRYMPAMFSMHMAAHMLLSMLAPILL
                     ALGGPVTLALRALPAAGRDDSPGPREWILIALHSKLSRFFTHPLVATIIFVVGFYALY
                     FGGIFDAFAGNHAAHVLMNLHFLLSGYLFYWVVIGIDPTPRPIPPVAKLGMVFASLPL
                     HAFFGVVLMGTSTVMAERFYRSLQLSWHTDLLGDQRLGGGIAWAAGEIPLAVVMIALL
                     IQWSRSDERTAKRLDRAAERDEDADLAAYNAMLAELARRDSGSRSQP"
     gene            16160..16633
                     /locus_tag="VF874_09475"
     CDS             16160..16633
                     /locus_tag="VF874_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9176296.1"
                     /translation="MFETPITVVGRIITDLRRRQVGDQEVISFRMASNSRRRGADGNW
                     EPGNTLYITVNCWGKLVTGVGAALGKGRAVIAVGQVYTSEYDDSNGVRRSSLEMRATS
                     VGPDLSQSLVRILDKVKDADPAPASENDAESSDETAADADDDGVTVAGKALPLSA"
     gene            16701..18377
                     /gene="ettA"
                     /locus_tag="VF874_09480"
     CDS             16701..18377
                     /gene="ettA"
                     /locus_tag="VF874_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX9176297.1"
                     /translation="MAEYIYTMRKVRKAHGDKVILDDVTLSFLPGAKIGVLGPNGAGK
                     SSVLRIMAGLDQPNNGDAFLATGATVGILQQEPPLNEEKTVRGNVEEGLGELKVKLDR
                     FNEVAELMATDYSDELMEEMGQLQEELDHADAWDIDSQLEQAMDALRCPPPDEPVTHL
                     SGGERRRVALCKLLLSKPDLLLLDEPTNHLDAESVQWLEQHLASYKGAILAVTHDRYF
                     LDNVAQWILELDRGRAYPYEGNYSTYLEKKTERLAVQGRKDAKLQKRLQDELAWVRSG
                     AKARQAKSKARLQRYEEMAAEAEKTRKLDFEEIQIPTGPRLGNLVVEVEHLDKGYDGR
                     VLIKDLSFTLPRNGIVGVIGPNGVGKTTLFKTIVGIEKPDSGTVKIGDTVKLSYVDQA
                     RANIDPNKTVWQTVSDGLDYIEVGQNEIPSRAYVSAFGFKGPDQQKPAGVLSGGERNR
                     LNLALTLKQGGNLLLLDEPTNDLDVETLGSLENALEKFPGCAVVISHDRWFLDRTCTH
                     ILSWEGDASNEAKWFWFEGNFAAYEENKVDRLGPDAARPHRVTHRRLTRD"
     gene            18435..23279
                     /locus_tag="VF874_09485"
     CDS             18435..23279
                     /locus_tag="VF874_09485"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX9176298.1"
                     /translation="MTVRSESVDGAGGWDNFAPGLMRAYVDSYRGPQGDAPVIGERGL
                     TAPAQIRAQQVVTPALLAAHGRLGFSRRQGTTNVATYGDDDRHGFGPALQLITDETPM
                     LLESVTVLLNRLGVAYVAILSPVFRVRRRPDGVLEDIRPRRPSGDTSGGVDEAWIHVE
                     LAVGADRKALEEVEHQLPSVVADAQLVARDSAAMRDALHRLAGGLERDNSGQFPAAQR
                     HDVAQLLRWLDDGHFVLVGYQRCSVSGGDANVHDSSRLGVLRRRQDVFPQLTGSHELF
                     LLTQATVPSYLRYGAYPYVVVVRDNAGRDAIEHRLVGLFTVAAMNANVLEIPVISRRV
                     VAALERSGRDLKSLPGQLLLDVIQTFPRSELFALDVDELLKMATAIIDVGSRRRTLLF
                     MRADQLGHFVSALVYLPRDRYTTAVRLHMMDILVHELGGTSIDYTARVSESPWAAVHF
                     TIRLPDQARQHAVDTSQQNRQRIQSLLSEAARTWADRLLGAVRFGSIGQEQAEHYAEA
                     FPEVYKQAVPPDDAIAHIGIIEELQDDSVKLVFAQDDSEERTAQLTWFLGGQSASLSQ
                     LLPMLQSMGVIVLEERPFTVTRADGLQVWIYQFKIALHPTIPEPPEDSDADETAQRFA
                     DAVTAIWRGQAETDRFNELVLRAGMTWQQVVILRAYAKYLRQAGFPYSQAHIEAVVND
                     NPHTARSLVALFEALFDPSDSTKRDAQAAAAAVAKDIDALVSLDTDRVMRAFASLVQG
                     TLRTNYFVTRDGSARRQGVLSLKLDPSVIDELPLPRPKFEIFVHSPRVEGVHLRFGPV
                     ARGGLRWSDRREDFRTEILGLVKAQAVKNAVIVPVGAKGGFVVKQPPMATGDASADRE
                     ATRDEGVACYKMFIAGLLDVTDNVDRSSGKVTSPDRVVRRDDDDAYLVVAADKGTATF
                     SDIANKIAKSYGFWLGDAFASGGSVGYDHKEMGITARGAWEAVKRHFREMGVDTQSED
                     FTVVGIGDMSGDVFGNGMLLSKHIRLIAAFDHRHIFLDPNPDAATSWEERKRLFDQPR
                     SNWEDYDTSLISEGGGVFSREQKSITVSHQVRRALGIADDVTEVTPPELVRAVLCAPV
                     DLLFNGGIGTYIKAEYESNVDVGDRSNDTVRVNGNQVRAKVIGEGGNLGVTSLGRVEY
                     DLAGGRINTDALDNSAGVDCSDHEVNIKILVDSLITAGKVSEDERTQLLESMTDEVAQ
                     LVLANNSEQNDLMGTSRANAASLLTVHARQIRDLEKNRGLNRELEALPSDKETRRRMD
                     QGLGLTSPELATLMAHVKLHVKDRVLASELPDQEVFASRLPVYFPTKLRDNFASDIRS
                     HQLRREIVTTMLVNDVVDCGGITFVYRVVEEAGVGPVDSVRTFVAADAIFGVSDTWRR
                     IKAAREESAIPTMLSDRMTLDLRRLLDRASRWLINYRPQPLAVGAEINRFRSQVRGLS
                     PRIAEWLRADDRQIVVNKANDLVSHGATEDVAGAVAAGLYSYSLLDVIDIADIRDRDP
                     AEVADTYFALMGHLGIDGLLTAVSDLERNDHWHALARLAIRDDIYGSLRSLCFDVLAV
                     GEPDENGEEKIAEWEHTNTSRVDRARRTLTEIYESEQHDLATLSVAARQIRSMTRAGS
                     GTSA"
     gene            23276..23725
                     /locus_tag="VF874_09490"
     CDS             23276..23725
                     /locus_tag="VF874_09490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9176299.1"
                     /translation="MSVPRDEPLARRASNTFTTPVPVRWSDIDMYQHINHATMVTILE
                     EARVPFLREPFGAEITTVGLLIHEVRVVYRGQLRLVDSPLQVTIWTERLRAVDFTLGY
                     EVRPVNADLGSKPSVIAETQLVTVHIEEQRLQRLSPSQRDYLKRWMR"
     gene            23722..24357
                     /locus_tag="VF874_09495"
     CDS             23722..24357
                     /locus_tag="VF874_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176300.1"
                     /translation="MSNVGERGVWLPDAADRANLAAFVERARRLDDAGVIRLRQRSED
                     VLVAWAATGFDVLASRVVAGSVRPPDICAGADELLRGLAASGVSGFVDTGFPMDSAWR
                     GPLPPESGFAHVDDVPVPVVLELAQRGAEVSKEQGAQGPPVSLLDQEVIQVSGAGVTV
                     GIPMRCVFALTAMGFVDSPDGVVRVRTVGPWLRIDAPYGSVYYRRDLLRLV"
     gene            complement(24413..25987)
                     /locus_tag="VF874_09500"
     CDS             complement(24413..25987)
                     /locus_tag="VF874_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEX9176301.1"
                     /translation="MGDLDGRSWWSRAVFYQVYPRSFADSNGDGVGDLDGVAAKLDYL
                     TLLGVDAIWLNPVTVSPMVDHGYDVADPRDVDPLFGGLDALDALIVAAHQRDIKVTMD
                     LVPNHTSSQHPWFQAALAAGPGSAQRERYIFRDGRGRGGALPPNNWISVFGGPAWTRV
                     TEPAGTPGQWYLHLFDAAQPDLNWDNPEVFDDLEKTLRFWLDRGVDGFRIDVAHGMAK
                     APGLPDMEVAENKLLSETGDDPRFNNESVHEIHRFIRRVLDDYDGAVAVGEVWVYDNA
                     QFAAYLRHDELHLGFNFRLVRAEFDAAGIRSAIDNSLAAVALAGATPTWTLSNHDVER
                     EVTRYGGGEVGLTRARAMVLVMLALPGAVFIYNGEELGLSNVDLPDEVLQDPVWFRSG
                     GRERGRDGCRVPMPWSGDEPPFGFSDNPDTWLPMPPEWAPLTVERQLRNPDSTLNFFR
                     RALDVRRGFTGTALEWVDAPMGALVFRVDGLVCSLNAGEKPIEIPSGELLLASGPLVD
                     GRLPPDTAAWLVERPT"
     gene            complement(26034..>26584)
                     /locus_tag="VF874_09505"
     CDS             complement(26034..>26584)
                     /locus_tag="VF874_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176302.1"
                     /translation="DGYSGTTLTKIAAAAGVSVETVQGHGPKAALLTAAAEYAAFGVT
                     GEGNILNLDVGRKLLAIDDREEALDFLAAAQADIHQRTARLVQTLSAAASSDPKLDRY
                     LSDVIASINLQTRRILDAYRDRGWLRDDVPFDELVETSAVLCSTETFLRIVHRDGWSA
                     EAYRAWCRRMLAETVFLPPQAD"
BASE COUNT         4404 a         8647 c         8887 g         4646 t
ORIGIN      
        1 ctgatcctgc gcctgctgat ggacggtctg acgcacgccg cgatcgtcat gcgctagaca
       61 gcgcttcagc atcttcttcg tcccgggccc accgaactcg agttggcgca agctgctgag
      121 gccatggcgc gcgaaaccga cccgctgctc ggcccactgg ttgaagacct gctacgggtg
      181 gcgctccgcc actcgttcca aaccgaggcg gtcaacgcca ccgagcgggc atccggtgca
      241 ctgccaggcg cgcgtgacgt ggcggtggcg ttcgctgacc tcgtcgggtt cactcgcctc
      301 ggtgaggcgg tcggcccgga agacctcgcc cgcctggcca gcggcctggg tgaccgggct
      361 cgagaggtcg cgcaggaccc cgttcagttc gtcaagacga tcggcgacgc ggtcatgttc
      421 gtcagcacgg agccgctggc actgctgcac agcgtcctcg acctcgtcga cgccgtcgaa
      481 gcggacgcgc tgccccggct gcgggtgggc gtcgcgtacg gtccggccgc cagctacgcg
      541 gccgactggt acggcagacc cgtgaacgta cagccgcatc accgcggtcg caccgccgtg
      601 ggcggtgtac gccgaggagt cggctcgccg tgcgaacggc gatgccgtgg gcatcgcctg
      661 gcaggagatc ggaccgcggc gcctcaaggg tgtccgcggc gacgtgcggc tgtttccagg
      721 cggcgcgcgg cagcgcctga cagggtccca agcagttctg gaaaaagtgt cacctgtggc
      781 cggggtgcgt gctcctacct gtgtcatccg acgccgccac agaaaaggaa cagcaatgac
      841 caccacccac aacacccgta tccgccgcat cgtgcgcacc gccgtgttcg ccggcgggct
      901 ggccctggcc gtcagcccgt ttgccagcct cgcggtcgcc aacgcccagt acaaagagag
      961 tgacttcgca gcgtgcctgg agcgcgatat gcccacggac tactgctgcg agcacgccgg
     1021 cggagtcatg aggaacggcg catgcatcaa tccagacgac ctgatggtgc cctcctctgg
     1081 cgtcttcgag cagacgccga ctacgccaac cacgcggccg ccggtggtgg cgaccgtccc
     1141 cacaggcgtt ttcgagcagg tcaccacggc gacgccgaca cccaagcccg gttcggtggt
     1201 caccgcgccg atcaccacgg ccactaggta acaaccctgc tgacaccggc cagcttggcg
     1261 cctgccaggc tggccggtgc cgctatcgtt tggggatggg cggcgactcg gcagctacgc
     1321 gaagcccgtt cggcgcctcg gcgtttcgcg gccgtcgacc cacccgttcc ggcgatctcg
     1381 cggtcgagac gcacgggatt gcgcccatcg gcgaagacca gcggtacggc gcacccgcgc
     1441 gattgttcac tgtttggttc gcgccccagg tgaacatgac cggcgtcttc accggcacct
     1501 tggccatcac gttgggtttg ggtttctggc tcgggcttct tgccatggtc atcgggaccg
     1561 tgctcggctc cttcgtggtc ggttacctgt cgacgtgggg tccgcggacc ggcacggctc
     1621 agctgccgat ctcaaggatg gcgttcggcg gcggcgtcgt cctgcctgcc accctgcagt
     1681 ggctgtcgtc gatcgcgtgg gacgggctgg tgggtctctt cggcgccgac gcactcgcgg
     1741 tccttctcgg cattccgttc tggctagccg tgctgattgt gctggcggtg cagggggtcg
     1801 tcgggttctt cggctacgaa gtcatccacc gtctacaagc ggtgctgacc gtcgtcttgt
     1861 tcgtcacatt cgtcgtgttc gccgtcaaac ttgtcggcgg ccacgagatc gtcacacccg
     1921 ccggcgtgca gggccccgac ctcctcggag ctttcgttct cgaggtgacc gtggcactga
     1981 gcctggcgat ttcctgggcc agctatgcgt ctgacttcag ccgatacctg cccgcgaact
     2041 cgtcgcagct aaaggtcttc ggtttcacgt tcgccggcat cgtgtcggcg tacgtgttcg
     2101 ttcagggcat tgggttagcg gccgctccgg cgctctcgga gcagacctcc gagggcgtgc
     2161 ggtcggtgat gggcggtgga gttctgggcg cgctggcgct gctcgtcatc gcgctggcgg
     2221 cggtcggatc cgcggcgatg aacgactaca gcggatcgct ggcgctgcag accgccggcg
     2281 tccgggtgcg ccggccggtg tcggcacttg tcgtcaccgt gctcgcgttc gcgctgattc
     2341 tgtggctgca ctccggcgac atgtcgcagc ggttccagga cgtgctgctt ttggtcagct
     2401 actggattcc cggattcgtc gcggtcaccg tcatcgactg gtcgatccgc gcgcggggac
     2461 ggcacaccat caatcccgcg gcggagaaca ccgaccggct ggacgctgtg gcagcgctgg
     2521 tcacgttcgt cgtcgcatat ggcgccgcgg tgccgttcat gcacaccacg ctgatcgagg
     2581 ggccggtcgc ccgcgcatgg cacggcgccg acaccgccta cttcgtcggc ttcctcgtgg
     2641 cggcactgct gtacggggga taccgctggc gttctatgtc gcgtagttga agtcctcgtc
     2701 cggctcggtg ccgaatcgga atctgcgtcc actgatctcc gacggggtga tccggacgaa
     2761 atggcgtttc ctcgtcgcta tccacggcag caactgcgcc cgctcggctt cggcgatctc
     2821 ctcctgggtg cgcaccatct gagcctgccc cttgacgatg atgctccagc caccctcgaa
     2881 gttgtgctcg tcgacctcga acgccacccg gttgttcgcc acggcgctcg cgagcttggt
     2941 gccctcggca gtgcgaaaca gcacgctacg gcgttgcacg acgaagttga ccggaaagat
     3001 ctccggctgc cctccgacac tcgtggcaag tcggcccagc gtcctgctgg ccagcagttg
     3061 ccagctctcg tcttccggga tgacgacgat tggctcgacc ggcgcagaca tggctcagtt
     3121 ccgccgagga agcgaagcga cgtatggggt gtcaacgccg acaggaccga ggccctccgc
     3181 accgcccgga gatcccagcg gttcgtcgcg gccgggcagc gtctcctcga agaacgcggc
     3241 gttgtccgcg aggtgctgca gctcgtcctc cggcaggtcg gtctcgtagt agatcgcgcc
     3301 gacctcgcag atcagcctgc aggcaccgca gtccacgcac tcgttcgggt tgatgtagag
     3361 cgagcgttcg ccctcgtaga tgcagtcgac cggacactcc tggacacact gccggtgcat
     3421 cacgtcgacg cattctctgc cgatcacata tgtcatgacc gcaggatacg agtccgctgc
     3481 ccgttacagc ccgtgcggaa agccattgcc tgagaggact aatggccctt ttttcgttac
     3541 ccaacgttta cttgtcgtcg gtgtaaattc acgctcgcga aggcatttca cagggaaagg
     3601 gggccccatc gcggacgcgt gagaggacac tcgtgtgttt gtgggggttt cttgtgaagc
     3661 agtccgcgag tgtcccgcaa tcgcccttga aattcagacg tctttcactc cttcgacggg
     3721 ggtcgtctca tgagtgtggt gtcgccgagc caggatctgt acgggttcgc cattgagcta
     3781 cgaaaactgg cgtacaccat gcctggcggg caggaggatc cgctgatccg gctcagcgag
     3841 cgcatggttc gccacgcacg agaacaggct tcgcgagagt cgccgccgct ttaggtcagc
     3901 gaatcggtcg gctggttgtc cgagcccatc ccgggggccg ttggctgacg cgcgtaagtt
     3961 ggtcccggtg acgagcaccg agttggcggt tccgaaggac tgggacgaga tcacgccgga
     4021 ttggatgacg gccgcgttgg cggctcgcca tcccgacgcc accgtcgagg cggtcaccgt
     4081 cgagttgcgt gatgacggca caaaccggcg ggctcgcctg ggtctgacgt actcggcagg
     4141 taccggcccg gcatcggtgt tcgtcaaggc cgccgaccct gctcacaaag atctgatccg
     4201 gctgaccagc ggcatgtttc acgagccacg gttatttcag tcgggtgtgg agctgccgct
     4261 ggagcacccg accgtgtacg ccgcactcat cgacgaggcc gactacgact tcatcctggt
     4321 catggaggac ttgacgacgc gcggcgccga cccgcgtgat gcgacgcggc cgatgacggt
     4381 cgaacaggtc gccaccggcg tgcgggggct gggtcggatg cacggacagt tctgggggcg
     4441 cagggtgatc gagcatccgg cgctgggctg gctggagccg tttgtgccgt gggacgggat
     4501 gcaggcggct ccgcttcccg aggcgttgag ccggctgggc gacgacgccc cggccgaggt
     4561 gatgtcgctg acgatcgaag ggctcatcga ggacacctgg aagccgttca tccgcagtct
     4621 gactgcctcg ccgcagacgc tgctgcatgg tgacccgcat atcgggaaca cctatctgtt
     4681 gcccagcggc gaagtgggat tcctggactg gcaggtcgcc cgccgcggca actggtcgtt
     4741 ggatctcggc tacttcctgc agggcgcgtt gaccatcgag gaccgccgcc gcagcgaacg
     4801 gcagctgctc gaggagtacc gcgatgcgct ggcgttgcct gccgacgagt taccgtcgat
     4861 cgacgagatc tggttgcggt accgcgcgtc ggtcgctcac gggctgaccc tgtggctgtg
     4921 tacggccagc gcaggagaac tgtggcagcg gcccgacatc gcgatcgcgc tggcgcagcg
     4981 ctactccgct gcctacgcgg atctggacgc ccgtgctgcg atcgccgaga tcgcatgatt
     5041 tctcgagtcc gacgggcgct gcggccttca gcggcagcgg tctaccgagt tgtgtgatat
     5101 ttcgtgcatg tatgttgcga tactcctacg gatcgacatg atcaaatgtg atgacggcag
     5161 ggcgttcccc gttgccgcgc aatacaactg ggacatcaac ggaaacggga ttgccagcgc
     5221 cttcaacgac ggatcctgtg gcgacgaacc tggacggatc gaagcgggat tcacgctgac
     5281 acaggtggct taaggaccga tccacggccc ggcgccgaac gccaagagaa atccattcgg
     5341 agcaccctcg agctcccagt attggacaac ggcggtccca aacaccctct gacctagcgc
     5401 ccccggcacg actcgaacgt gcgacctagg gattagaagg cccttgctct atccagctga
     5461 gctacggggg caacgcgcga agtctagcca accgccgatt acgttgcgcc gtagatgccg
     5521 gcgcgcacgc cggcacccca tggcgccgca ggctcaggcg ctgcgcgcac cagccttcag
     5581 tttggctatc acctgttcga ggttgaagct ggctgactcc tgccgtcgcg gaaactcggc
     5641 gagcgtctgg atcatctggg cgacgtaggt ctgcgccgga acgaacagga aaatgtggtc
     5701 gagaacccaa tcgtagtagg tgttcgaggt ttgatcggcc ttctcgtacg gatctgtccg
     5761 caggttgaac agcttgggga agcgcaactc ggtgtagggc tcggcccaga tctgcaaggt
     5821 gccagggcac cgctgctcga gaaagacgaa cttccagttg tcgtaccgca aggctgtgag
     5881 gtcgccgtca tcggagcagt agaagaagtg ctgacggggg ctctcgtcaa cctcgccggt
     5941 gatgtagggc aattggttgt gcccgtcgag gtgcaccttg taggtggtgc cgttgaggtc
     6001 ggcgcccttg agcagtttgt cggcgatatc gggtaccccg gcggctgcca gcagtgtgac
     6061 gaaccagtcg ttgtggctga cgatgccgtt gagcaccttg ccggcgggaa tgtgccccgg
     6121 ccagcgcatc agggccggga cgcggtaggc gccttcccag ttcgagttct tctcattgcg
     6181 gaacggggtc atcccggcat cgggccagga gttcatgtgc gggccgttgt cggtggagta
     6241 catgacgatc gtgccctcgg cgatgccaag ttcgtcgagc aggtccagca ggctgccgac
     6301 ggtgtcgtcg tggtcgagca tcgtgtcgtg gtagttggac tgccagcgcc cagcgcggcc
     6361 gatgctctcg ggcttggtat gtgtgcggaa gtgcatgtgg gtggagttga accagacgaa
     6421 gaacggcgtc tgggccgcgt gctgccgttt gatgaagtcg acggcggcat cgcggaactc
     6481 ctcgtcgcag gtctccatcc gcttcttggt gagcgggccg gtgtcctcga ttcgctgagt
     6541 gccgtctggg ttggcccagc tgtgcaggac gccgcggggc ccgaatttct tccggaactc
     6601 cgggtctttc ggatagtcgg gtagctcggg ctcctcttcg gcgttgaggt ggtagaggtt
     6661 gccgaagaac tcgtcgaagc cgtgtgccgt gggcaggtgc ccgtcgcggt caccgagatg
     6721 gttcttgccg aactggccgg tggcgtagcc ctgcgccttc aaggccgtgg cgatggtggg
     6781 gtcctctggc tgcagcccaa gcgtggcccc gggcataccg accttcgtca ggccggtgcg
     6841 gtacgggttt tggccggtaa tgaaggcggc ccgacccgcg gtacagcttt gctctccgta
     6901 atagtcggtg aagcgcaccc cctcgtccgc gatccggtcg atgttgcggg tgcggtatcc
     6961 catcacacca tcgctgtagc agctcaggtt gctgagtccg atgtcatcac cccagatgac
     7021 tagtatgttc ggcttgtcgc tcacgggaat cccttcggtg tgggggagcc cggacgctta
     7081 tcacgggcac ggccgagact gatttatgtc tcgaggccgc agtggagatg ctagcctcgt
     7141 ccatcgcacc ccatgcagtt tcgattgaca cggcgcgtca tgtgtctgct gcctactttc
     7201 gttgcggccc tgaccatttg ccggacccaa gcaacgaacg cgttctgacc gccaagccag
     7261 cccgcatgga accactgtgt ctgcggttgg ttatcggtta tttagagatg tagacgagac
     7321 ttcgttcatg gacgattcac agagcgaacc gcaatcccgt cggctgacga ccccgagggc
     7381 tgccgcggtc gcgggcatcc ttttcgccgc gttcttcgca tccagcttgg tgctgttgcg
     7441 cagcgcaatt cccgatgatc cgttcgcgca gaccgcttgg gtcacccgtg gcgaaacgag
     7501 gatcactgtt gcattgatcc tggcccctct cgcgggcatc gcgttcctct ggtttgttgg
     7561 ggttcttcgc gatcggttcg gcgtactgga ggaccgcttc ttctcgactg tatttctggg
     7621 tagcggactg ctgttcttgg ccatggtgtt cgtttcgatg gcgaccgccg gggcaatact
     7681 gactgtctcc cagactccga cgacgccctc gaacgggctg gtctacttcg gtcgatctct
     7741 gatgctgcag atcagcaacg tctgggcgtt gcgtatggcg gcggtattca tgatctcgct
     7801 ggccaccatc tggatacgca cccggctgat gccacgctgg ttggcggcac tcacctacct
     7861 gctcgccgtc acactgctgt tggtggtgag ttacagcctg tgggtgacgc tggttttccc
     7921 ggcgtgggtt ggcgtcgtca gtctcttcat cgtcatccgc gatcgagagc agcggcgcga
     7981 gctcgggcca ccgcaagatc gctgattcaa acaagtgtcg gtggaggtta cgagctcgcc
     8041 gccggaacgt tggtgcgacc gaaccggttg gcggtcaccg aggccagcga caatccctcc
     8101 cactccgcag gtaggcggac tagcgttgtg ctcggtgcgg ttacgggaga ggtgaaagag
     8161 acggtggttg acgcagtgga cgcggccggt ctgccggaag aactcggcgc gttcgacatg
     8221 ttgctgcacc gcggtgaagc gaacccgcgg acgcgctcgg gaatcatggc cctcgaaatc
     8281 ctggacacga cgcccgactg gaaccgattt gtcgccgtgt tcgagcacgc gtcgcgtcag
     8341 gtgctgcggc ttcgtcagaa ggtcgtggtg ccgacgattc cgacggcctc gccgcgctgg
     8401 gtcgtggacc cggacttcaa cttgaacttc cacgtccggc gcctccgcgt gcccgaaccg
     8461 ggaacgctgc gcgaggtgtt cgacatcgcc gaggtgagtt tgcagtcgcc gctggacatt
     8521 tcgcgcccgc tgtggaatgt cacgttgctc gaggggctcg ttgacggtcg ggctgcgctg
     8581 ctgctgcaca tgagccacgc cgtcaccgac ggcgtgggtg cggtcgaaat gttcgcccac
     8641 atctacgacc tggaacgcga tccgcctgcc cgcgagcccc ttccgctgcc gatcccgcag
     8701 gacctcaccg gcaacgatct ggcccgcgag ggcctgaacc ggttgccgct gaccatcctc
     8761 ggcggcgtgc ggggcgcggt gtccggcgcc gcccaagtgg tcggacgcgt gctgaccaac
     8821 ccggtctcag ccgtcgaggg agtagtcgag tacgcccgct ccggcgccag ggtcatgtcg
     8881 acggtggccg agcattcccc gctgctgcgc cgccgcagcc tctcctcgcg tagcgaagca
     8941 atcgacatga agctcaccga catccgcgcc gccgccaagg ccgccgaggg ctccatcaac
     9001 gacgcgtacc tcgccgcgct gtgcggcgcg ctgcggttct accaccagga gctcggcgtc
     9061 ccgatcgact cacttcccat ggcggtcccg gtgaacctgc gagccgaggc cgaccccgcg
     9121 ggcggcaacc gctttgccgg tgtgatgatc tcggcgcccc tcgggctctc cgacccgaaa
     9181 gcgcgcatcc acaacgtccg tacccaaatg tgcaagcggc gtgacgaacc cgcgatcgac
     9241 atgatcggtg ccgtcgcacc gctgatcggc ttgttgccgg agccgctggt ggaatcgatc
     9301 gcgagttcgg tggtgtcctc agacgtgcag gccagcaacg tgccggtgta cgcaggcgac
     9361 accttcatcg cgggtgcaaa agtgttgcgg cagtacgggc ttgggccgct acccggtgtc
     9421 gcgatgatgg cggtgctgat ctcccgcggc ggcatgtgca ccgtcaccac tcgctacgac
     9481 cgggcgtcat tcaccgacga cgatctttgg gcccgttgcc tgttgaccgg gttcaacgac
     9541 gtgctggcgc tcggcgggcc gggccgcgcc aggcccgcgt ccttcgacga gcccgcagcc
     9601 tccatccagc caccatcacc caacgggagt tcgccgtcat gagcccctcc aacaaccggt
     9661 cgctcgatcg ccgcgacatg cgcctgcccg gctcggtggc cgagatcgag gccagcgcgc
     9721 ccggtccgaa agtcggcgct ttcttcgacg tcgacggcac gctcgtcgcc gggttcaccg
     9781 gggtgatcat gacgcgagac cggttccgcc gccgcgaaat cagcatcggc gagttcctcg
     9841 gcgtcgtgca ggctgcgttg aatcaccagc tcggtcgcgc ggacttcgag cagctcatct
     9901 accggggtgc ctcgatgatg aagggacgct cgctcgacga catcgacgag ctcggcgagc
     9961 ggctgttcgt ccagcacatc gtctcgcgga tctatcccga gatgcgtgag ctggtccgcg
    10021 cccaccaggt tcgcggccac accgtcgcgc tcagctcgtc ggcgctgacc gtgcaggtcg
    10081 aacccgtggc tcggttcctg ggcatcgaca acatcttgag caaccgcttc gaggtcgacg
    10141 agcacggcgt gctgacaggc ggcgtgctca agccgatcct gtggggtccc ggcaaggcca
    10201 acgccgtgca gaagttcgcc gcggacaacg acatcgactt gaaaaagagc tacttctacg
    10261 ccgacggcga cgaggacgtc gcgctgatgt atctcgtcgg caacccaaga cccacgaacc
    10321 ctaagggcaa gctcgcggcg gtggccgcca agcgcggatg gccggttctg cgcttttcca
    10381 gccgcagcgg cagcagcccg acctcggcgc tgcgcacact cgccgggatc gcgtcgatgg
    10441 cgccggtggc cgcgggcgcg gtcggagtcg ggctgctggc gggcagcaag cgtcgcggtg
    10501 tcaacttctt tacttccaat ttcggccgag cgctgctggc cgccaccggt gtcaacgtca
    10561 acgtgatcgg cgaggagaac ctgaccgcgc agcggcctgc ggtattcatc ttcaaccacc
    10621 gcaaccaagt tgacccgatc gtcgccggat acctagtccg cgacaacttc acgtcggtgg
    10681 ggaagaagga actcgagagc aacccgatcg ccggcgcgct gggcaagatc atggacgcgg
    10741 cgttcatcga ccgtgacgac cccaaggccg ctgtcgaagg gctgcacaag gtcgaggaac
    10801 tggcgcgtaa aggtctatca atcttgatcg ccccggaggg cacccggctg gacaccaccg
    10861 aggtcggccc gttcaagaag ggcccgttcc gcatcgcgat gtcggtaggc atcccgatcg
    10921 tgcccatcgt gatccgcaac gccgaagtca tcgccgcgcg cgattcgagc acgttcaacc
    10981 ccggcacggt cgacgtcgtc gtctacccgc ccatcccggt cgacggctgg actcacgaca
    11041 acctcccgga acgcatcgcc gaagtccgtc agctcttttt ggataccttg acgaactggc
    11101 cgcgcgacga agtccccgag acgaagctga cgtcgcgcgc aacgccgaag aaggcgcctg
    11161 cgaagaaagc gcctgcgaag aaggccgccg cgaaaaaggc tcccgcgaag aagaccgcag
    11221 cgaaaaagac tgcggacgat ggcaattcac aagcagccaa gaaggcgacg ccgaccaagg
    11281 cgaaggggag gcgatgagcg cccggaccga cgacctggac agtttcaccg ccaccgacga
    11341 cgcgttggtc ctggcggcgg tgtcgtcacc ggcagaactc gagttgctga acgactggct
    11401 cgcccatcag cggcgtgagc atcccgagac caaggtcgag gtgctcgaat tgcctgccga
    11461 agacgacccg gcacccgggg tcgtggctca gctcgccgag cagctcgccg ccgacgaaga
    11521 ccgcaccgtc gtgccggtgc gcgtgttctg ggtgcccggc gggctgccta cgcgagtcaa
    11581 gctggtgggc ctgctctccg gccgtgacgc gtaccgtccg ccacagatcc tgcaacgtcg
    11641 gatcctgcac cgagacgcat cgagggccag gatcgtcgcg ggcgaaccgg ccaaggtttc
    11701 cgagcttcgt caacagtggc aggacacgac ggtcggccat gatccgcgtg acttcgcccg
    11761 gttcgtgttg cggcgggccg cgctggccat cgaacgtgtc gaactacggc tgctcgggcc
    11821 cgcgtacaag tccccgaggc tggtcaaacc ggaaatgctc gcctcggcgc ggtttgtcga
    11881 agggctgcaa cggattcccg gcgcgaccgt cgagaaggcc gggcagatgc tcgacgaact
    11941 ggccaccggg tggagccggt tctccgtcga tctgatcccg tcgctgggcc gcgcgatctt
    12001 cagccgcggc ttcgacccca acatcgacta cgaccgcgcc gagatcgagt cgatgcgcca
    12061 agcgctggaa acgctccctg cggtgctgct gttttcgcac cggtcttacc tcgacggggt
    12121 gatcgtgccg gtggcgatgc aggagaaccg gctacctccc gtccactcgt tcgccggcat
    12181 caatttgtcg ttcggattca tgggaccgct gatgcgccgc tcgggcgcca ttttcatccg
    12241 gcgcaagatc gacgacccgc tgtacaagta cgtgctgaag gagttcgtcg gttacatcgt
    12301 cgagaagcgg ttcaacctga cgtggtcgat cgagggcact cggtctcgca ccggaaagat
    12361 gttgccgccc aagctcggcc tgctcacctt cgtcgcggac gcgtacctcg acggccgcag
    12421 cgatgacatc ctgttacaac cggtgtcgat cagtttcgac caactgcacg agaccgccga
    12481 gtacgccgcg tacgcacgcg gcggcgagaa aacccccgag ggcctcagtt ggctgtacaa
    12541 cttcatcaag gcgcaggggg agcgcaacta cggcaagatc tacgtccgct tccccgcagc
    12601 ggtgtcgatg cgcgggtatc tcggcgagcc acacggtccg tcggtcaccg acgaggccgc
    12661 caaacgccta gcgctgcaga agatggcgtt cgaggtggcg tggcgcatct tgcgcgctac
    12721 tccggtgaac gccaccggct tggtgtcggc gctgctgttg accacgcggg ggcgagccct
    12781 gactttggac cagctgcacc acacgttgca ggattcgctc gactacctgg aacgcaagca
    12841 gaccccgatg accaacagcg cgctgcggct gcgcacgaac gagggtgtgc gcgcggcggt
    12901 ggacgcgttg tcccacggcc atcccgtcac ccgcatcgac accggccgcg aaccggtgtg
    12961 gcggatcgcc cctgaggacg agcacgaggc agcgttctac cgcaacacgt tgatcgacgc
    13021 gttcctggaa acgtcgatcg ccgaactcgc gctcgcctac gcggcacacg ccgagggcga
    13081 ccgcgtaacg gcgttctggg accgagtgac gcggctgcgc gacctgttga agttcgactt
    13141 ctacttcgcc gactcggcag cctttcgcga gcacatcgcc gaggagatgg cctggcacga
    13201 cggctgggag gcgcaggtgc gcgccggcgg ccacgacatc ggcgagttgt tgcacgcgaa
    13261 acaaccgctg atgtcgcctg cgatgctgcg cccgttcctc gaggcctacg agatcgtcgc
    13321 ggacgtgctg cgcgacgcgc ccgccgaaat cggcgagaag gacctgacca agctcgcgct
    13381 gggcgtcggc aagcagtatt cggcccaagg ccgggtgcgc agcaacgagg cggtgtcggc
    13441 gctgttgttc gcgacggcgc ggcaggtggc cgccgaccag ggactgctgc agaccgcacc
    13501 ggatctcgcc gagcgtcgcc gggcattcct ggccgagctc cgcagcattt tgcgcgacat
    13561 ggacatggtc gagttggcgt cactgcggca gttcaccgag cgggaggcgc agcgggggcg
    13621 tgagcgtctc gaggccggct gaggtcttag tcgtcggcgt gacggcgctg ttcccagcgt
    13681 tccaccgaat cctgcacaag gtgcaatacc agcgcggcca acggaatgct gaccgacaac
    13741 agcgctacca caacaacatt catcgtcgtc tcctgtccgg gagattacgt cgaggtgaac
    13801 gtacgcccac ggaccgacac ggcttttggg gccgcagatt gataacggca cgcgcacact
    13861 tcgagttcga cgttttgtgc ggcccatccg atgcgggggc cagcggaggt tgcagacgcc
    13921 cggccatacc ctggattgat gacggcagcc ccgaccagca cgcgtcgtgc agcggtctgg
    13981 ccggtgctca ccggtgtggc ggtgctggcc ggcgtcacgg ccaccgcgat cggcgcgatg
    14041 tccctggcgg ctgcgctgac ggcaaccggc ttgccggatc cgggatccgt caccacctac
    14101 gggctgccct tcgtccgcgc cgcgggtgag gtggccgcgg tgatcgcggt cggctcgttc
    14161 ttgttcgcgg cgttcttcgt gccgccccaa gacaacggcg ttttggacgc cgcgggttac
    14221 cgcgcggtgc gaatcggaac ggtggcgtcc ggcgcgtgga cggtgtgcgc ggcggtgctg
    14281 gtgccgctga ccatctccga cgtctccggt cagccggtgt tcgaacacct caacccgctg
    14341 acgatctggt cggcggccgg cctcgtcaac accgcgggcg cttggcgatg gaccgcattc
    14401 ctcgcggcca tggtgacgat cgcgagcctg cccgtgctgc gctggtcgct gacccctttc
    14461 ctgttcgccg gctcgctgct gacgttgatg ccgctgggcc tcactgggca ctcgtcggcc
    14521 ggcggtgccc atgacgtggg aaccaacagc ctcgtcatcc atctggtcgc cggtgcgttg
    14581 tgggcgggtg gcctgctggc cttgctcacc cacgcgctgc gcggcgcaga acacgcggac
    14641 ctcgccgcgc gccgtttctc taaggtggcc ggctggtgtt tcgtcgcgat ggcggtcagc
    14701 ggcgtcgtca acgcgctggt ccggatcacg ccgggcgacc tcctcgacac cacgtacggc
    14761 cgactcgtgc tggccaaggt ggctgcgctg ggcgcgctcg gtgtgctggg ctggcggcag
    14821 cgccgcagcg ggctcgtcgt gctgcgcgac aaccccaccg cgcgcggccc gttggtccgg
    14881 ctcgccctga tcgaggcgct ggtcttcggc gtcacgttcg gcatcgcggt cggtctgggt
    14941 cgaaccccgc cgccggcacc accgatcgga tacccgtcga tccccgaagt cgagatcggc
    15001 tacgacttcg ccgggccgcc gacgctggcg cgggtactgt tcgactggcg gttcgacctg
    15061 ttcttcggca ccgcggcaat cgtgctggcg gtggtgtacc tggccggggt gcgtcggctg
    15121 cgccggcgcg gcgacacctg gaaggcgggc cggacgttcg catggctgtg cggctgcgcg
    15181 gtgctgctgt tcgcgacgtc gtcgggcgtg ggtcgctata tgccggcaat gttctccatg
    15241 cacatggcgg cgcacatgct gctgtccatg ttggcgccga tcctgctggc gctgggcggc
    15301 ccggtgaccc ttgcgctgcg ggcgctgccg gccgcgggcc gcgacgactc gcctggcccg
    15361 cgcgaatgga tcctgattgc gttgcacagc aagctttctc gcttcttcac gcatccgctc
    15421 gtcgcgacca tcatcttcgt cgtcggcttc tacgcgctgt acttcggcgg catcttcgac
    15481 gcattcgccg gcaaccacgc tgcgcacgtg ctgatgaacc tgcacttcct gctcagcggc
    15541 tacctgttct actgggtggt gatcggcatc gacccgacac cgcgccccat cccgccggtc
    15601 gcgaagctcg gcatggtgtt cgcgtcattg ccgctgcacg cgttcttcgg agtggtgctg
    15661 atgggcacca gcaccgtgat ggccgaacgg ttctaccgct cactgcagct gagctggcac
    15721 accgatctgc tcggcgatca gcggcttggc ggcggaatcg cttgggcggc aggcgaaatc
    15781 ccgttggccg tggtgatgat cgcgctgttg atccagtgga gtcgcagcga tgagcgcacc
    15841 gccaagcggc tggaccgggc ggccgagcgc gacgaggacg ccgacctggc cgcctacaac
    15901 gcgatgctcg ccgagctggc taggcgggac tcgggaagtc ggtcacagcc gtaacacgaa
    15961 ggggcagtgc tgcttttcgg aattgcgata tcacccgcga tatcgttttg cggcaagacg
    16021 aactcacttc aatagtgctt atccacatcg cgggtttcat ccacaggcgt ggagttcgac
    16081 gtgggggatc gacgtgcatt gtcggtgccc aagcgctcaa tggcggtgac atcacgcaag
    16141 tgaatgaaag gaaaccgcaa tgtttgagac accgatcacc gtcgtaggcc gcatcatcac
    16201 cgacctcaga cgccgccagg tcggcgacca ggaagtgatc agcttccgga tggccagcaa
    16261 ttcccgccgc cgtggcgccg acggcaactg ggagcccggc aacacgctct acatcaccgt
    16321 caactgctgg ggcaagctcg tcaccggcgt cggcgccgcc ctaggcaagg gccgtgcggt
    16381 gatcgccgtc gggcaggtct acaccagcga gtacgacgac agcaacggcg tccggcgttc
    16441 gtcgctggag atgcgcgcca cctccgtcgg cccggatctt tcgcagtcac tcgtgcgcat
    16501 cctcgacaag gtcaaggacg ccgacccggc gcccgccagc gagaacgacg ccgaatcgtc
    16561 tgacgagacg gctgctgacg cggacgacga cggtgtgacc gtcgccggga aggcgctgcc
    16621 tctgtcggct tagcgctgtc gggcggtgcc taggatggtc cggagactca ccctgaccac
    16681 tgaaaggcac tacccacggc atggccgaat acatctacac gatgcgcaag gtccgcaagg
    16741 cgcacggcga caaggtcatc ctcgacgacg tcacgctgag cttcctgccg ggcgccaaga
    16801 tcggcgtcct cgggccgaac ggggccggca agtcgagcgt cttgcggatc atggcggggc
    16861 tggaccagcc caacaacggc gacgcgttcc tggccaccgg ggcaacggtc gggatcctgc
    16921 agcaggagcc gccgctgaac gaggagaaga cggtccgcgg caacgtcgag gagggcctcg
    16981 gcgagctcaa ggtgaaactc gaccggttca acgaggtcgc cgagctgatg gccaccgact
    17041 actccgacga gctgatggag gaaatgggcc agctgcagga ggagctggac cacgccgatg
    17101 cgtgggacat cgactcgcag ctcgagcagg ccatggacgc gctgcgctgt ccaccgcccg
    17161 acgaaccggt gacgcacttg tccggcggcg agcggcgccg cgtcgcgctg tgcaagctgc
    17221 tgctctccaa gcctgacctg ctgctgctcg acgagccgac gaaccacctg gacgccgaga
    17281 gcgtgcagtg gctcgagcag cacctggcca gctacaaggg cgcgatcctg gccgtcaccc
    17341 acgaccgcta cttcctggac aacgtcgcgc agtggatcct cgagctggac cgcggccgcg
    17401 cgtacccgta cgagggcaac tactcgacgt atctggagaa gaagaccgaa cggctggccg
    17461 tgcagggtcg caaggacgcc aagctgcaga agcggctgca ggacgaactc gcctgggtcc
    17521 gctccggagc caaggctcgc caggccaaga gcaaagcgcg gctgcagcgc tacgaggaga
    17581 tggccgccga agcagagaag acgcgcaagc tcgacttcga ggagatccag atccccaccg
    17641 gcccacggct gggcaacctg gtggtggagg tcgagcacct cgacaaggga tacgacgggc
    17701 gggtgctgat caaggatctg tccttcacgc tgccgcgcaa cggcatcgtc ggcgtgatcg
    17761 gacccaacgg cgtcggcaag accacattgt tcaagaccat cgtcggcatc gaaaagccgg
    17821 acagcggcac ggtgaaaatc ggtgacaccg tcaagctcag ctacgtcgac caggcccggg
    17881 ccaacatcga cccgaacaag acggtatggc aaacggtttc ggacggcctc gactacatcg
    17941 aggtcggcca gaacgaaatc ccgtcgcggg cctacgtgtc ggcgttcggg ttcaaaggac
    18001 cggatcagca gaaacccgcg ggcgtgctct ccggtggaga acgcaaccgg ctcaacctcg
    18061 cgctgaccct caagcagggc ggcaacctgt tgctgctcga cgagcccacc aacgacctag
    18121 acgtcgagac cctgggctcg ctggagaacg cgttggagaa gttccccggg tgcgccgtcg
    18181 tgatctccca cgaccgctgg ttcctcgatc gcacctgtac acacatcttg tcgtgggagg
    18241 gcgatgcctc caacgaggcg aagtggttct ggttcgaagg caatttcgcg gcctacgagg
    18301 agaacaaggt ggataggctc ggacccgacg ccgcgcgtcc gcaccgcgta acgcaccgca
    18361 ggctgacacg cgactagggt gagcggcggt tgcgtgccag cgtgggcacg ctgcgccgaa
    18421 tgaggagccc tcccatgacg gttaggtccg agagcgtcga tggcgcgggt gggtgggaca
    18481 acttcgcgcc gggcctgatg cgggcctatg tcgatagcta ccgcgggccc cagggcgacg
    18541 ccccggtaat cggcgaaagg ggtctgaccg cgccggcaca gatccgtgct cagcaggtgg
    18601 tgacgcccgc gttgctggcg gcccacggcc ggctgggttt ctcgcgaagg caaggaacca
    18661 ccaacgtcgc cacctatggg gacgacgacc gtcacggatt cggtccggcg ctgcagctga
    18721 tcaccgacga gacgccgatg ctgctggagt cggtgactgt gttgctcaac cggctcggcg
    18781 tggcctacgt cgcgatcttg agcccggtgt tccgggtacg gcgccgcccc gacggcgtgc
    18841 tggaagacat ccgcccgaga cggccgagtg gcgacacatc cggcggtgtc gacgaggcgt
    18901 ggatccatgt cgagctcgcc gtcggcgctg atcgcaaggc cctcgaggaa gtcgaacacc
    18961 agctgccgtc ggtggtcgcc gacgcccagc tggtggctcg cgattccgct gcgatgaggg
    19021 acgcgctgca caggctggct ggagggctgg aacgcgacaa ctccggccaa ttccccgccg
    19081 cgcagcgcca cgacgtcgcg cagctgttgc gctggcttga cgacggccac ttcgtgctcg
    19141 ttggctacca acgctgttcg gtgtccggcg gcgacgccaa cgtccacgac tccagccgcc
    19201 tgggcgtgct gcgccgccgc caggacgtct tcccgcagct caccggctcc cacgaactgt
    19261 tcctgttgac gcaagccacg gtgcccagct atctgcgcta cggcgcctat ccttacgtgg
    19321 tcgtcgtccg cgacaacgcg ggcagggacg cgatcgagca ccggctggtc gggctgttca
    19381 ccgtcgccgc gatgaacgcg aatgtgcttg agatcccggt catctcgcgc cgggtcgtcg
    19441 cggcgctgga aagatccggc cgtgacctga aatcgttgcc gggccagctg ctgctcgacg
    19501 tcatccaaac cttcccgcgc tccgagcttt tcgcactcga cgtggacgag ttgctcaaga
    19561 tggccaccgc gatcatcgac gtgggctcgc ggcgccgcac gctgcttttc atgcgcgcag
    19621 accagctggg gcatttcgtc tcggcgttgg tctatctgcc tcgcgaccgc tacaccaccg
    19681 ctgtgcgact gcacatgatg gacatcctgg tacacgaact cggcggcacc agcatcgact
    19741 acacggcccg agtcagcgag tcaccatggg ccgcggtgca tttcacgatt cggttgccgg
    19801 accaggcacg acagcacgcg gtcgacacgt cgcagcagaa ccggcagcgg atccagtcgc
    19861 tgctctccga ggcggctcgc acctgggcgg atcgcctgct cggggccgtg cggttcggct
    19921 cgatcggcca ggagcaggcc gagcattacg ccgaggcctt ccccgaggtc tacaagcagg
    19981 ccgtgccacc cgacgacgcg atcgcgcaca tcggcatcat cgaagagctg caggatgatt
    20041 cggtcaagct ggtcttcgcg caggacgaca gcgaggagcg taccgcgcag ctgacgtggt
    20101 tcctcggcgg ccagtcggcg tccctgagcc agctgctgcc gatgctgcag agcatgggcg
    20161 tcatcgtgct cgaggaacgg ccgttcacgg tgacgcgtgc cgacgggctg caggtgtgga
    20221 tctaccagtt caaaatcgct ttgcacccga cgattcccga gccgccggag gacagcgacg
    20281 ccgacgagac tgcgcagcgg ttcgccgacg ccgtcaccgc gatctggcgc gggcaggccg
    20341 agacggaccg gttcaacgaa ctcgtgctgc gtgccgggat gacctggcag caggtcgtca
    20401 tcctgcgcgc gtatgcgaaa tatcttcgcc aggccggttt cccgtacagc caggcgcaca
    20461 tcgaagcggt cgtcaacgac aacccgcaca ccgcgcgctc gctggtcgcg ctgttcgagg
    20521 cgttgttcga cccgtcggac tccaccaaac gcgacgcgca ggcggcggcc gcggcggtgg
    20581 ccaaggacat cgatgcgctc gtcagcctcg acaccgatcg ggtgatgcgg gccttcgctt
    20641 cattggtgca ggggacgctg cggactaatt acttcgtgac gcgtgacggc tctgctcggc
    20701 ggcagggagt gctgtcgctg aagctcgacc ccagcgtgat cgacgaattg ccgttgccgc
    20761 ggccgaaatt cgagattttc gtgcactcgc ctcgggtcga aggcgtgcat ctgagattcg
    20821 gcccggtcgc acgcggcggg ctgcggtggt cggaccggcg cgaggatttc cgcaccgaaa
    20881 tcctcggctt ggtcaaggcg caggcggtga agaacgcggt catcgtcccg gtcggcgcca
    20941 agggcggctt cgtcgtcaag cagcctccga tggccactgg cgacgcaagt gccgaccgcg
    21001 aggcgacgcg cgacgaaggc gttgcctgct acaagatgtt catcgccggg ttgctcgacg
    21061 tcaccgacaa tgtcgaccgc tcgtccggca aggtcacgtc accggatcgg gtggtccgcc
    21121 gcgacgacga cgacgcgtac ctcgtcgtcg ccgccgacaa gggcaccgcg acgttctccg
    21181 acatcgccaa caagatcgcg aaatcctacg ggttctggct gggcgacgcg ttcgcgtcgg
    21241 gcggctcggt gggttacgac cacaaggaga tgggcatcac cgccaggggc gcctgggaag
    21301 ccgtgaagcg gcacttccgt gagatgggcg tcgacaccca aagcgaagac ttcaccgtcg
    21361 tcgggatcgg tgacatgagc ggcgacgtgt tcggcaatgg gatgctgctg tccaagcaca
    21421 ttcggctgat cgccgccttc gaccaccggc acatcttctt ggaccccaac cctgatgccg
    21481 cgacgtcgtg ggaggagcgc aaacggctct tcgaccagcc gcgctccaac tgggaggact
    21541 acgacacgtc gctgatcagc gagggcggcg gcgtgttcag ccgtgagcag aagtccatca
    21601 cggtcagcca ccaagttcgc cgggcgctgg gcatcgccga cgacgtcacc gaggtgaccc
    21661 ccccggagct ggtccgcgcc gtgctgtgcg caccggtgga tctgctgttc aacggcggca
    21721 tcggcaccta catcaaggcg gagtacgaat ccaacgtcga cgtcggtgac cgctccaacg
    21781 acaccgtgcg ggtaaacggt aatcaggtgc gcgccaaagt gattggtgag ggcggcaacc
    21841 tcggtgtcac ctcgctgggg cgggtcgagt acgacctggc ggggggccgc atcaacaccg
    21901 atgcgctgga caattcggcc ggcgtcgact gctccgacca cgaagtcaat atcaagatct
    21961 tggtcgactc gttgatcaca gcggggaagg tctctgaaga cgaacgcaca cagctgctgg
    22021 agtcgatgac cgacgaggtc gcacaactgg tgttggcgaa caactccgag cagaacgacc
    22081 tgatgggcac cagccgcgcc aatgccgcca gcctgctgac cgttcacgct cgccaaatcc
    22141 gcgacctcga gaagaatcgc ggactgaacc gcgagctgga ggcgcttccg tcggacaagg
    22201 agacccggcg gcggatggat caggggctgg gcctgacgtc accggagctg gcgacgttga
    22261 tggcgcacgt gaaactgcac gtcaaagacc gggtgctcgc cagtgaactg cccgaccagg
    22321 aagtgttcgc gtcgcggctg ccggtgtatt tcccgacgaa acttcgggac aacttcgcca
    22381 gcgacatccg ctcgcaccag ctgcgccggg aaatcgtcac cacgatgttg gtcaacgacg
    22441 tggtggactg cggcggcatt accttcgtct accgggtggt ggaggaagcc ggggtcggcc
    22501 cggtggattc ggtgcgcacg ttcgtggcgg ccgacgcgat cttcggagtc agtgatacct
    22561 ggcgtcgcat caaagccgcc agggaagaaa gcgccatccc cacgatgctg tctgatcgca
    22621 tgacgctcga cctgcgtagg cttctcgacc gcgcatcgcg gtggctgatc aactaccggc
    22681 cccagccgct ggcggtcggc gcggagatca accgtttccg atctcaagtc cgggggctgt
    22741 cacctcggat cgccgaatgg ctgcgcgccg acgaccgcca gatcgtcgtc aacaaagcca
    22801 acgacctcgt atcccacggc gccacagagg atgtggccgg cgcggtggcg gccggcttgt
    22861 acagctacag tctgctcgac gtgatcgaca tcgccgacat ccgcgatcgt gaccccgcgg
    22921 aggtagccga cacatacttc gcgttgatgg gccacctcgg tatcgacggc ctgctcaccg
    22981 ccgtgtcgga tctcgagcgc aacgaccact ggcatgcgtt ggcgcgcttg gccattcgag
    23041 acgacatcta cgggtctttg cggtcgctgt gcttcgacgt gctcgcggtc ggggagcccg
    23101 acgagaacgg cgaagagaag atcgccgagt gggagcacac caatacgtcc cgggtagacc
    23161 gtgcccgtcg gaccttgact gaaatttacg agagcgaaca gcacgatctc gcgacgctgt
    23221 cggtggcggc gcggcagatc cgcagcatga cgcgggcggg cagcgggacc tccgcatgag
    23281 tgttccgcgc gatgagccgc ttgcgcgaag agcatcaaac acattcacca caccggtgcc
    23341 ggtgcggtgg tccgacattg acatgtacca gcacatcaac cacgcgacga tggtgacgat
    23401 cctggaagag gcgcgggtgc cgtttctgcg cgagccgttc ggtgccgaga tcaccacggt
    23461 cggtctgttg atccacgagg tgcgggtggt ctaccgaggc cagctgcggc tcgtcgactc
    23521 gccgctgcaa gtcaccatct ggaccgagcg attacgtgcg gtggacttca cgctcggcta
    23581 cgaggtgcgc ccggtcaacg ccgacctggg ttcgaaaccg tcggtgatcg ccgagacgca
    23641 gctggtgacc gtgcacatcg aagagcaacg gctgcaacgg ctctcgccgt cgcagcgcga
    23701 ctacctgaaa cgctggatgc gatgagcaat gttggggagc gtggcgtgtg gctgcccgac
    23761 gccgcggatc gagcgaacct cgcggcgttc gtcgaacggg cgcgccgact cgacgacgcc
    23821 ggggtgattc ggctgcggca gcgctccgag gatgtgctgg tcgcttgggc ggcaacggga
    23881 ttcgacgtac tggctagccg tgtggtcgcc ggctcggtga ggccgcctga catctgcgcg
    23941 ggtgcggacg agctgctacg cgggttagcg gcgtcgggtg tgtccggctt tgtcgacacc
    24001 ggttttccga tggactcggc ttggcgcggg ccgttgccgc cggagtccgg attcgcacac
    24061 gtcgacgacg tgccggtgcc cgtggtgttg gagctggcgc agcgcggggc cgaggtatcc
    24121 aaggagcagg gcgcgcaggg gccgccggtg tcgctgctgg accaggaagt gatccaggtc
    24181 agtggcgccg gagtgacggt gggcatcccg atgcgttgcg tgttcgcttt gacggcaatg
    24241 ggtttcgtcg acagtccgga cggcgttgtc cgggtgcgga cggtggggcc gtggctgcgc
    24301 atcgacgcac cgtacggttc ggtgtactac cgcagggacc tgctgcggtt ggtgtaagtc
    24361 cgccgaacgt gcgcacactg cgagatatga gccccaaatt ttcgccgtgg gctcacgttg
    24421 gacgctctac aagccacgcg gcggtgtcgg gcggcagccg tccgtcgaca agcggcccgc
    24481 tggcgagaag cagctcgccc gaagggatct cgatcggctt ttcaccggcg ttcagcgaac
    24541 acaccaaccc gtctacccgg aaaaccagcg cgcccatcgg cgcgtcgacc cattccaacg
    24601 ctgtccccgt gaatccgcgc cgtacatcga gtgcccgccg gaagaagttc agcgtcgaat
    24661 cgggattgcg cagctggcgc tcgacggtta gcggcgccca ctcagggggc atgggcagcc
    24721 aggtgtcggg attgtcggag aacccgaacg gcggctcgtc gcccgaccac ggcatcggta
    24781 cccggcaccc gtcgcggccg cgctcacggc ccccggaccg gaaccacact gggtcctgca
    24841 gcacctcgtc cggcaagtcg acattcgaca gcccgagttc ttccccgttg taaatgaaca
    24901 ccgcccccgg cagcgccagc atgaccagca ccatggcccg cgcccgcgtc aacccgacct
    24961 cgccgccgcc gtagcgggtg acttcacgct cgacgtcgtg attggacagc gtccacgtcg
    25021 gggtcgcgcc cgccagcgcc accgcggcca gggagttgtc gatcgcgctg cgaatcccgg
    25081 ccgcgtcgaa ctcggcgcgc accagccgaa agttgaatcc caagtgcagt tcgtcgtggc
    25141 gcaggtacgc agcgaactgc gcgttgtcgt acacccacac ctcgccgacg gccaccgcgc
    25201 cgtcgtagtc gtcgagcacc cgtcggatga agcggtggat ctcatggacg ctttcgttgt
    25261 tgaaccgcgg gtcgtcgccc gtctccgaca acaacttgtt ctcggcgact tccatgtcgg
    25321 gcagccccgg cgccttggcc atgccgtgcg cgacgtcgat gcgaaacccg tcgacaccgc
    25381 ggtccaacca gaaccgcaac gtcttctcca ggtcgtcgaa cacctcgggg ttatcccagt
    25441 tgaggtccgg ctgcgcggcg tcgaacaggt gcaggtacca ctggccgggg gtacctgccg
    25501 gctcggtcac ccgggtccag gccgggccgc cgaacaccga gatccagttg ttcggcggca
    25561 gcgcgccgcc gcggccgcgc ccatcgcgga agatgtagcg ctcgcgctgg gcggaaccgg
    25621 gcccggcggc gagtgcggcc tgaaaccacg ggtgctgcga gctggtgtgg ttggggacga
    25681 gatccatcgt caccttgatg tcgcgctggt gggcggcgac gatcaacgcg tccagtgcgt
    25741 cgagaccgcc gaagagggga tcgacatcac gggggtcggc gacgtcatag ccgtggtcga
    25801 ccatcggcga cacggtgacc gggttgagcc agatcgcgtc gacgccgagc agcgtcaggt
    25861 agtcgagctt ggccgcgacg ccgtccaagt cgccgacgcc gtcgccgttg ctgtccgcga
    25921 acgaccgcgg atacacctga tagaacaccg cgcgtgacca ccacgatcgg ccgtcgagat
    25981 cacccacgag cgatcattgt gccgcagcga caacacgtcc gcgcggcccg ctgtcaatcc
    26041 gcttgcggcg gaaggaaaac cgtctccgcg agcatgcgtc gacaccaggc ccggtaggcc
    26101 tcggcgctcc agccgtcgcg gtggacgatg cgcaagaagg tctcggtgct gcagagtacg
    26161 gctgatgtct cgacgagttc gtcgaacgga acgtcgtcgc gcagccaccc tcggtcgcgg
    26221 tatgcgtcga ggatgcggcg ggtctgcagg ttgatgctcg cgatcacgtc gctcaagtac
    26281 cggtccagct tgggatcgga gcttgcggcg gcgctgaggg tctgcaccag tcgcgccgtc
    26341 cgttggtgga tgtccgcttg tgccgcggcg aggaaatcca aggcttcttc acggtcgtcg
    26401 atggccagca acttgcgccc gacgtccaaa ttgagaatgt tgccttcgcc ggtcacgccg
    26461 aacgcggcgt attcggcggc agcggtcagc agggctgcct tcggtccgtg gccttggacc
    26521 gtttcgacgg agacgccggc ggccgcagcg attttcgtca gggtggtgcc tgagtacccg
    26581 tcgg
//
LOCUS       DASWBF010000259         9210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1009289,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000259 DASWBF010000000
VERSION     DASWBF010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009289"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VF874_09510"
     CDS             <1..140
                     /locus_tag="VF874_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176303.1"
                     /translation="SSAADSRIIVRPYGEDGVRVTIAAPHENDAFLDFARRWMGDHRV
                     R"
     gene            130..609
                     /locus_tag="VF874_09515"
     CDS             130..609
                     /locus_tag="VF874_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4334 domain-containing protein"
                     /protein_id="HEX9176304.1"
                     /translation="MSDELARKTFTELKERTGQIPDAELDDFWASLQPATIEFVLGDW
                     KGGEFNTGHRRNGLLARLNWFGKTFNSATDAKPLVCLDADGNKFSNTEAMNGEASLWM
                     EEFRGGVTASMVYDGKPVHDHFKKIDDDAVIGIMNGKGAVDVSSGTGRYLYFYLERV"
     gene            complement(866..1447)
                     /locus_tag="VF874_09520"
     CDS             complement(866..1447)
                     /locus_tag="VF874_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEX9176305.1"
                     /translation="MPTEMPPGAETPPVDELTSAEASLAELQRVVHPIAANDLGKQTP
                     CREFDIEALTDHLMNSITTIGGVAGAEFPARDATDSVERQIILAARPALDAWRRRGLE
                     GTVQLGPNEVPACVMAGILSIEFLIHAWDYAQATGQTVDPTPELADFVFGLARKIITP
                     QGRGNVGFDDPVDVPEDASAFDRLIAFTGRRPG"
     gene            complement(1463..1687)
                     /locus_tag="VF874_09525"
     CDS             complement(1463..1687)
                     /locus_tag="VF874_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176306.1"
                     /translation="MVVAFDGAGTSDVLSGADVLVRDVVVGGEVDVDVAPPGSVVIAT
                     DPSVVRTQAASPRHMTAVATTRTGTPIPQP"
     gene            1686..1874
                     /locus_tag="VF874_09530"
     CDS             1686..1874
                     /locus_tag="VF874_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176307.1"
                     /translation="MKCDEFTKLDQPERLAVVKEIIDQENSPFGALGDEFAESMASTM
                     CQFMPDQTVREVLTGSPP"
     gene            1914..2738
                     /locus_tag="VF874_09535"
     CDS             1914..2738
                     /locus_tag="VF874_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9176308.1"
                     /translation="MGDAYESVTVDIDGHVAQVTLVGPGKGNAMGPAFWSEMPDVFAQ
                     LDADRDVRAIVLTGSGRNFSYGLDLPAMGGTLGDVMSDGTSARPRAEFHATVLRMQQS
                     ISAVADCRTPTIASVHGWCIGGGVDLISAVDIRYASADAKFSVREVKLAMVADVGSLA
                     RLPLILTDGHLRELALTGKNIDAARAEKIGLVNDVYDDAEACLAAAHATAAEIAGNPP
                     LTVRGIKDVLDQQRIARVSESLRYVAAWNAAFLPSKDLTEGITATFAKRPPKFTGE"
     gene            complement(2863..2949)
                     /locus_tag="VF874_09540"
     tRNA            complement(2863..2949)
                     /locus_tag="VF874_09540"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2913..2915),aa:Ser,seq:tga)
     gene            complement(2979..3491)
                     /locus_tag="VF874_09545"
     CDS             complement(2979..3491)
                     /locus_tag="VF874_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9176309.1"
                     /translation="MEVLIGGRSAAEMPGLDIAEERSWQNFLEAAVRMSAMLNRGLVD
                     THRLTLNDVRLLDMLKKSATGSARMGDLAEQLISLPSRVTRQIRRLEAQGLVRREASP
                     DDRRGVLATITDEGRATVDQAMMTYGQYVKAHFLSQLSRPQVAALGENCRRINHGLKM
                     ASPPRRLGRI"
     gene            3558..4541
                     /locus_tag="VF874_09550"
     CDS             3558..4541
                     /locus_tag="VF874_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX9176310.1"
                     /translation="MRAVVAHSSDQLSWEEVPDVHARKGEVLLRVRAAGVNRADLLQA
                     AGHYPPPPGASEILGMEVSGVIANVADGVTTWSPGQEVCALLAGGGYAEYVAVPAPQL
                     MPVPAEVDLEAAAGLPEVACTVWSNLVMTAGLHEGQTVLLHGGGSGIGTHGIQVARAL
                     GARVAVTAGSAKKLDAARRLGADITINYRDEDFVDRIREETDGAGVDVILDIIGAKYL
                     DRNIDALASDGRVVVIGMQGGTKGELNLGKLLAKRAGVIATALRGRPVDGPASKGAIV
                     KEVVAHVWQMIADGRVRPVIGAVLPIEQAAEAHRLLSSGEVTGKVVLKVGD"
     gene            complement(4538..5734)
                     /locus_tag="VF874_09555"
     CDS             complement(4538..5734)
                     /locus_tag="VF874_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX9176311.1"
                     /translation="MVYDVARVRGLHPSLGDGWVHLDAQLGMLVPDSVATTVSTAFRG
                     SRPDSRGPHPAARRSAAVLEAARQAVADLVNADPRGVVLGADRAVLLRALADASASRA
                     GLGYEVVVSRLDDEANIAPWLRAANRYGARVKWAEVDIETGDLPSWQWESLITKVTRL
                     VAITSASSTLGTVTDLRAVTKLVHDVGGLVVVDHSAAAPYRLMDLNEVDADVVAVNAV
                     AWGGPPIGALVFREPSMIDTFASMSLAPNAAGPARFETGVHQFGFLAGVVASIEYLAS
                     LDESASGSRRQRLAFSMQSAGLYMDRVFDYLQVSLRSLPRVMVLGRPEVRIPVLSFAV
                     TEVPAERVVQRLADNGICAIANAPSQVLDLIGVNDIGGAVTVGLAHYSTTAEVDQLVR
                     ALASLG"
     gene            5843..7795
                     /locus_tag="VF874_09560"
     CDS             5843..7795
                     /locus_tag="VF874_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HEX9176312.1"
                     /translation="MSLALGVLPALLLLIVPGALVGTAARLPWPVAVAVGPALTYGIV
                     GLAIVPLGAIGWPWNAWTALFAMAVVTGAVAGLRVVLSRYRDRDAERLAVSGWPVLIV
                     AAGIVLGAGLIGYAAVVGLPHWQSVPSTWDAVWHANEIRYILDTGQASPTHMGELRNV
                     ETHATLYYPSAFHALAAVLSQLTGAAATTAYTVSSVAAAVWLFPVSAAVLTWQLLRSR
                     TDRWRTAGCAAAAAAMSASFTAVPYIEFDTASMPNLAAYGMAAPTMVLIVSTLRHRDR
                     IPVAVLALLGVFSVHTTAGVVVVLFVAAWWAVDGLWHPVRGRVADVVTLLLVALPAVL
                     LLLPQFGGILKQADIIVGHEFVTHAGKKRALFDAVVGHTRHLNDFPIQYALMALAAAG
                     GVILLLRKIWWPLAVWLLLVAAIVHSSAPFGTVLGSLLGKFSDLFYSDPRRLFAVITL
                     LLAPMAGIALFTLSAAAVAGVRRLVDRRISTRAGYGVTAVIVAAVSIGYAWSYFPRHE
                     FLLGQKYDSVIVDHKDLEAMAYLATLPGARNTLIADANTDGTAWMYAVAGLHPLWTHY
                     DYPVQQGPGYHRFILWAYADDADTDPRVAEAVRALGVRYVMTSTPVVREFVMPDGLAS
                     LDKSRSWTKIYDNGGARIYEWRGAGG"
     gene            7874..8395
                     /locus_tag="VF874_09565"
     CDS             7874..8395
                     /locus_tag="VF874_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HEX9176313.1"
                     /translation="MGMSTDDDDIEIINGRRDAADQQDDSDLRSVTDLVEQPAKVMRI
                     GTMIKQLLEEVRAAPLDDASRNRLREIHATSIRELEDGLAPELRDELERLTLPFTEDA
                     TPSDAELRIAQAQLVGWLEGLFHGIQTALFAQQMAARTQLEHMRQGVLPPGISMPGHQ
                     PSGPGHSGTGQYL"
     gene            8451..>9210
                     /locus_tag="VF874_09570"
     CDS             8451..>9210
                     /locus_tag="VF874_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9176314.1"
                     /translation="MRTQGAWVEFPIFDAKSRSLKKTFLGKAGGTIGRNTSNVLVIEA
                     LRDITLSLDTGDRVGLVGHNGAGKSTLLRLLSGIYEPTRGKAVVKGRVAPIFDLGVGM
                     DPEISGYENIIIRGLFLGETRKKMLSKVDEIAEFTELGDYLSMPLRTYSTGMRVRLAM
                     GVVTSIDPEILLLDEGIGAVDADFLKKAQARLQSLVERSGILVFASHSAEFLARLCKT
                     AMWIDHGVIRMSGGIEDVVRAYEGEDAARHVREVL"
BASE COUNT         1540 a         3067 c         3112 g         1491 t
ORIGIN      
        1 tctcgagtgc cgccgactcc cgtatcatcg tgcgccccta cggcgaggac ggcgtgcgcg
       61 ttaccatcgc ggcgccgcac gagaacgacg cgtttctgga tttcgcccga cgctggatgg
      121 gagatcatcg tgtcagatga gttggcccgc aagacgttca ccgaactcaa ggagcggacc
      181 ggccagattc ccgacgccga gctcgacgac ttctgggcct cgctgcaacc cgcgaccatc
      241 gaattcgtcc ttggggactg gaaggggggc gagttcaaca ctggtcaccg aaggaacggg
      301 ttgttggcgc gcctcaactg gttcggcaag acgttcaatt cggccaccga cgccaagccg
      361 ctggtgtgcc tggacgccga cggcaacaag ttctccaaca ccgaagcgat gaacggcgaa
      421 gcgagcctgt ggatggagga gttccgcggc ggggtgacgg cgagcatggt gtacgacggg
      481 aaaccagtcc acgaccactt caagaagatc gacgacgatg ccgtgatcgg aatcatgaac
      541 ggcaaaggcg ccgtcgacgt tagttctggg actggccgct acctgtactt ctacctggag
      601 cgcgtctaga ggggcctgct agacaacgct tgtcgttgac cgatcccctg ttcggcaggt
      661 tcatggccga ggccttcggc gggatagttc gacttgcccg cctctgcgcg acaacagcgt
      721 ttacccaggt cacggtcata acgtgagtag ctcgatcagc gacaacacag ggcttgcgcc
      781 gcaggtcaac gagttgtcgc gcagaggcgg gcaccaagat agtcggccag gaaagtgctc
      841 cgcagcgccg caattcagca ggttcctagc ccggccgccg accggtgaac gcgatcagcc
      901 ggtcaaacgc cgaggcatcc tcgggcacgt ccaccgggtc gtcgaatccc acgttgccgc
      961 gtccctgcgg cgtgatgatc tttcgcgcca gcccgaacac gaaatcggcc aactccggcg
     1021 tcggatccac cgtctggccg gtggcctgcg cgtaatccca agcgtggatg aggaattcga
     1081 tcgacaggat gcctgccatc acgcaagcgg gcacttcgtt cggccccagc tgcaccgtgc
     1141 cctcgaggcc gcgcctgcgc cacgcgtcca acgccggtcg cgcagcgagg atgatctgcc
     1201 gctccacgga atcagtggcg tcgcgcgcag gaaactccgc gccggcgact ccgccgatgg
     1261 tggtgatgga gttcatcaga tggtcagtga gcgcttcgat gtcgaactcc cggcacggtg
     1321 tctgcttgcc gaggtcattc gcggcgatcg ggtgcacgac ccgctgaagc tccgccaacg
     1381 aggcttccgc actggtcaat tcgtcgacgg gcggcgtctc ggcgccgggt ggcatttcgg
     1441 tgggcatgcc ccaacgctac gactatggtt gcgggatggg cgtgccggtg cgggttgtcg
     1501 cgaccgcagt catgtgtctg ggactcgcgg cctgcgtgcg gaccaccgag ggatcggtcg
     1561 cgatgaccac cgagcccggc ggcgcgacat ccacgtccac ttcaccgcct acgacgacgt
     1621 cgcgcaccag aacgtcggcg ccggagagca cgtcggaggt tccggcgccg tcgaacgcga
     1681 cgaccatgaa gtgcgacgag ttcacgaagt tggatcagcc cgagcggctg gccgtggtca
     1741 aagagatcat cgaccaggag aacagcccgt tcggcgcgct cggtgacgag ttcgccgaat
     1801 ccatggccag cacgatgtgc cagttcatgc ccgaccaaac cgtccgcgag gtgctcacgg
     1861 gttccccgcc ctgacgggag gcagtgtcca cccgctgatt agcctcttga cccatgggag
     1921 acgcatacga atcggtcacc gtcgacatcg acggtcacgt ggcgcaagtg acgctggtcg
     1981 ggccgggcaa gggcaacgcg atgggcccgg cgttctggtc ggagatgccc gacgtgttcg
     2041 cgcaactgga cgccgaccgc gacgtgcggg cgatcgtgct caccggttcc ggccgcaact
     2101 tcagctacgg gctcgaccta ccggccatgg gcgggaccct gggcgacgtg atgtccgacg
     2161 gcacatcggc gcggccccgc gccgagttcc acgccacggt gctgcggatg cagcagtcga
     2221 tcagcgcggt cgccgactgc cgcacaccga cgatcgcgtc ggtgcacggc tggtgcatcg
     2281 gcggtggcgt cgatctgatc tccgcggtgg acatccgtta cgcgagcgcg gacgccaagt
     2341 tctccgtgcg tgaggtcaaa ctcgcgatgg tcgccgacgt gggcagcctc gcccggcttc
     2401 cgttgatcct gaccgacggg catctgcgcg aattggcatt gactggcaag aacattgacg
     2461 ccgcgcgagc cgagaagatc ggcctcgtca acgacgtgta cgacgacgcc gaggcgtgtt
     2521 tggcggccgc gcacgccacc gcggcggaga tcgcgggcaa cccgccactg acggttcgcg
     2581 ggatcaagga cgtgctcgac caacagcgga tcgcccgggt ctcggaaagc ctgcgctatg
     2641 tcgcagcgtg gaatgcggcg ttcctgccgt cgaaagatct gacggaggga atcaccgcga
     2701 cgttcgccaa gcgtccgccg aagttcaccg gcgagtgacg acgtgagtgc tgcttctgac
     2761 atcgcccgcg ggagcgacgt tttgcagaag aagtgcgaga gagggcctgc aaaacgtcgc
     2821 tttcggcgcg atccggcgac gcgatccggc gacgcgagcg agcggtggcg gagggatttg
     2881 aacccccgga cggtgttagc cgtctctcgc tttcaaggcg agtgcattag gccgctctgc
     2941 cacgccaccg cggacaagcg tagccgtcgt tagcagggtc agatgcgtcc cagccgcctc
     3001 ggcggcgagg ccatcttcag tccgtggttg atccgccgac agttctcccc cagcgcggcg
     3061 acctgcggcc gtgacagctg gctcaagaag tgcgctttca cgtactggcc gtacgtcatc
     3121 atcgcctgat cgaccgtcgc cctaccctcg tcggtgatcg tcgccaacac gccgcgccgg
     3181 tcgtcggggc tggcctcacg gcggaccagc ccttgggcct cgagccgccg gatctgccgg
     3241 gtgacgcggc tcggcagcga gatcaactgc tcggccaagt cccccatccg tgcggagccg
     3301 gtggccgact tcttcagcat gtccagcagt cggacatcat tcagcgtcaa cctgtgcgtg
     3361 tcgaccaggc cacggttcaa catcgcagac atccgcaccg ccgcctccag aaagttttgc
     3421 caggatctct cctcggcaat gtccaggccc ggcatctcgg cggcggagcg tcccccgatc
     3481 aacacctcca tgggcgacca tcatatgtca gaaggcgttg ccaagcgacc aaaattgcgc
     3541 ggtagtaccg ttggggcatg agagcggtcg ttgcacactc atctgaccag ctgtcctggg
     3601 aagaagtgcc cgatgtgcat gcccgaaaag gcgaggtact actgcgtgta cgggcggctg
     3661 gagttaatcg tgctgatttg ctgcaggcag ccggccacta tccgccaccg cctggcgcaa
     3721 gcgagattct gggcatggaa gtgtccggag tgatcgctaa tgtggcagac ggtgtcacca
     3781 catggtcacc cggccaagag gtttgcgctt tgctcgcggg cggcggatac gcggaatatg
     3841 tggcggttcc ggcgcctcag ctgatgccgg ttccggccga agtggatctg gaggcggcgg
     3901 cgggcctacc agaagtcgcg tgcacggtgt ggtcgaacct ggtgatgacc gcgggtcttc
     3961 acgagggcca gacggtgctg ctgcacggtg gcggcagcgg catcggaacc cacggcatcc
     4021 aggtcgcccg cgcgctgggg gccagagttg cggtgacggc cggctcagcc aagaagctcg
     4081 atgccgcccg ccggctcggt gcggacatca cgatcaacta ccgagacgag gacttcgtcg
     4141 accggattcg tgaggagacc gacggcgcgg gcgtcgacgt gatcctcgac atcatcggcg
     4201 ccaaatacct cgaccggaac atcgacgcgc tggccagcga cggccgcgtg gtcgtcatcg
     4261 gcatgcaggg cggcaccaaa ggcgaactca acctcggcaa gctgctcgcc aaacgcgccg
     4321 gcgtgatcgc gaccgcgctg cgtggtcgtc ccgtcgacgg gcccgccagc aagggcgcga
     4381 tcgtcaagga ggtcgtcgca catgtgtggc agatgatcgc cgacggcagg gtgcggccgg
     4441 tgatcggagc cgtgttgccg atcgagcagg ccgccgaggc gcacaggctg ctgtcgtccg
     4501 gcgaggtgac gggcaaggtc gtgctgaagg tcggcgacta acctagggac gccagcgcgc
     4561 ggaccagctg gtccacctcg gccgtcgtcg agtagtgcgc gaggccgacc gtcaccgcac
     4621 cgccgatgtc gttcacaccg atcagatcca gcacctgaga cggcgcgttc gcgatcgcgc
     4681 agatcccgtt gtccgccagc ctttgcacca cccgctccgc cggcacttcg gtgacggcga
     4741 agctgagcac cgggatgcgc acctccggcc gccccagcac cattacccgc ggcaacgacc
     4801 gcaacgacac ctgcaggtag tcgaacaccc ggtccatgta caaccccgcg gattgcatcg
     4861 agaaggctag ccgttgccga cggctgccgc tggccgactc gtccagcgac gccaggtact
     4921 cgatgctggc gaccacgccg gcgaggaaac cgaactggtg cacgccggtt tcgaagcggg
     4981 cggggccggc ggcattcggg gccagcgaca tcgaggcgaa agtgtcgatc atcgacggct
     5041 cgcggaacac cagcgcgccg atcggcggac cgccccaggc gaccgcgttg accgccacca
     5101 cgtcggcgtc gacctcgttg aggtccatca ggcggtacgg ggcggccgcc gagtggtcga
     5161 ccaccaccag accgccgacg tcgtgcacga gtttcgtgac ggcacgcagg tcggtcacgg
     5221 tgccaagcgt cgacgacgca gacgtgatcg cgacgagccg agttaccttg gtaatcaagc
     5281 tttcccactg ccacgacggc agatccccag tctcgatgtc gacctcggcc cacttgaccc
     5341 tggcgccgta gcggtttgct gcgcgcagcc acggcgcgat gtttgcctcg tcgtcgagcc
     5401 ggctgaccac gacttcatag ccgagcccgg cccgcgaagc cgacgcgtcg gcgagtgccc
     5461 tcagcaaaac ggcgcggtcg gcgccgagca ccacaccgcg ggggtccgca ttcaccagat
     5521 cggccaccgc ctggcgggcg gcctccagca cggccgcgct gcgccgcgct gccgggtgcg
     5581 gaccgcggga gtccggccgc gagccgcgga aggccgtcga caccgtggtc gcgacggagt
     5641 cgggcaccaa catgccgagt tgagcgtcaa ggtgcaccca accgtcgccc aacgacgggt
     5701 gcaaaccacg cacccgggca acgtcgtaca ccatctaagc cacctcagag catctgaagg
     5761 atgtaagccg gcccagtcgc cggcggcgcc cgcgccgtcg cggtccgaca aaccgggtca
     5821 cctggccagc catactagtc cagtgagctt ggccctcgga gtgctgccag cactgctgtt
     5881 gctgatagtc cccggggcgc tggtcggcac tgccgcccgg ctaccgtggc cggtcgcggt
     5941 cgcggtcggt ccggcgctga cctacggcat cgtggggctg gcgatcgttc ccctcggcgc
     6001 catcggctgg ccgtggaacg cctggacggc gttgtttgct atggccgtgg tgaccggcgc
     6061 cgtcgccggt ttgcgggtcg tgctgtcgcg ctaccgcgac cgcgacgccg aacgcctcgc
     6121 cgtctccggc tggcccgtcc tcatcgtggc ggcagggatc gttctgggcg cgggactaat
     6181 cggttatgcc gccgtcgtcg gcctgccgca ctggcagtct gtcccgagca cctgggatgc
     6241 ggtgtggcac gccaacgaga ttcgctacat cctcgacacc gggcaggcgt cgccgacgca
     6301 catgggcgag ctgcgcaacg tcgaaactca cgcaacgctg tactacccgt cggcatttca
     6361 cgcgctggcc gcggtgctca gccagctcac cggcgccgcg gcgaccaccg cctacactgt
     6421 cagctccgtc gcggcggccg tgtggctctt cccggtgagc gccgcggtgt tgacgtggca
     6481 gcttcttcgg tccagaaccg accggtggcg caccgccggg tgcgcggccg cggcggcggc
     6541 gatgtcggcg tcgttcaccg ccgtgccgta catcgagttc gacaccgcgt cgatgcccaa
     6601 cctcgccgcc tacggcatgg ccgcgccgac gatggtgctg atcgtgtcga cgctgcggca
     6661 ccgcgaccgc atccccgtcg cggtgctcgc gctgctcggg gtgttctcgg tgcacaccac
     6721 cgccggtgtg gtggtggttc tgttcgtcgc ggcgtggtgg gcggtcgacg ggctgtggca
     6781 cccggtccgt gggcgggtgg ccgacgtggt gacgctgctg ctggtggcgc tacccgccgt
     6841 gctgctgctg ctgcctcagt tcggcgggat cctcaagcag gccgacatca tcgtcgggca
     6901 cgagttcgtc acccacgcgg gcaagaagcg ggcgctgttc gacgcggtcg tcggccacac
     6961 ccgtcacctc aacgacttcc cgatccagta cgcgctgatg gcgctggcgg cggcgggcgg
     7021 ggtgatcctg ctgctgcgca agatctggtg gccgctggcg gtgtggctgc tgctggtggc
     7081 ggcgatcgtg cactcgtcgg cgccgttcgg caccgtgctc ggcagtctgc tcggcaagtt
     7141 cagcgacctg ttctacagcg accctcgccg gctgttcgcg gtgatcacgc tgcttctcgc
     7201 gccgatggcc ggcatcgcgt tgttcaccct ttccgccgcg gccgtggccg gggttcgtcg
     7261 gctggtcgat cgccggatct cgacgagagc tggatacggc gtcaccgcgg tgatcgtcgc
     7321 cgcggtgagc atcggctacg cctggagcta tttcccgcgg catgagttct tactcgggca
     7381 gaagtacgac tccgtgatcg tcgaccacaa ggacctcgag gcgatggcgt acctcgcgac
     7441 gctgcccggc gcccgcaaca cgctgatcgc cgacgccaac accgacggca ccgcctggat
     7501 gtatgccgtg gcgggactgc atccgctgtg gacccactac gactatccgg tgcagcaggg
     7561 ccccgggtat caccggttca tcctctgggc ctacgccgac gacgcggaca ccgacccgcg
     7621 ggtcgccgaa gcggtacgcg cgctcggtgt tcggtacgtg atgacgagca cgccggtggt
     7681 ccgcgaattc gtcatgccgg atggccttgc gtcactggac aagtcacggt cgtggaccaa
     7741 aatctacgac aacggcggcg ctcgcatcta cgagtggcgc ggcgccgggg gttagggtct
     7801 tcgtcgccgg gtagaccgac tagtgtcagt cgggacgaac tccgaccgcg agctgggaaa
     7861 agaggtgacg acgatgggca tgagcaccga cgacgacgac atcgagatca tcaacggtcg
     7921 acgtgacgct gctgaccagc aggacgattc cgacctgcgg tcggtgaccg acctggtcga
     7981 acagcccgcg aaggtgatgc ggatcggcac gatgatcaag cagctgctcg aagaggtccg
     8041 cgcggccccg ctcgacgacg ccagccgcaa ccggctgcgc gagatccacg cgacgagcat
     8101 tcgagagctc gaggacgggc ttgctcccga acttcgcgac gagctcgagc ggctgacgct
     8161 gccgttcacc gaggacgcga cgccgtcgga cgccgagctg cggatcgcgc aggcacagct
     8221 cgtcggctgg ctcgaaggcc tgttccacgg catccagacc gcgctgttcg cccagcagat
     8281 ggccgcccgc acgcagctgg agcacatgcg ccagggtgtg ctgccgccgg gcatcagcat
     8341 gcccgggcac cagcccagcg gcccgggcca ctccggcacg gggcagtacc tgtagccgag
     8401 gcacggtgtc gctaaccgat tcgactgcgc cgcccgcgca cgacgtcagc atccggacgc
     8461 agggcgcctg ggtggagttt ccgatcttcg acgcgaagtc gcgctcgctg aagaagacat
     8521 tccttggcaa ggccggcggc acgatcggac gcaacacgtc caacgtgctg gtgatcgagg
     8581 cgctgcgcga catcaccctg tcgctggaca ccggcgaccg cgtcggcctc gtcgggcaca
     8641 acggcgcggg caagtccacg ctgctgcgcc tgctgtccgg aatctacgaa ccgacccgcg
     8701 gcaaggccgt cgtaaagggc cgcgtcgcac cgattttcga tctcggcgtc ggcatggacc
     8761 cggagatctc cggatacgag aacatcatca tccgcgggct gtttctcggc gagacgcgca
     8821 agaagatgct gtcgaaggtc gacgagatcg ccgagttcac cgagctcggc gattacctgt
     8881 cgatgccgct gcgcacctat tccaccggga tgcgcgtgcg gttggcgatg ggcgtggtca
     8941 ccagcatcga ccccgagatc ttactgctcg acgaaggcat cggcgcggtc gacgccgact
     9001 tcctgaagaa ggcccaggca cggctgcaga gcctcgtcga gcggtcgggc atcctggtgt
     9061 tcgccagcca ctccgcggaa ttccttgccc gactgtgcaa gaccgcgatg tggatcgacc
     9121 acggggtcat ccgcatgtcc ggcggcatcg aggacgtggt gcgtgcgtat gagggtgagg
     9181 acgcggcccg gcacgtgcgc gaggtgctgg
//
LOCUS       DASWBF010000260         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1210783,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000260 DASWBF010000000
VERSION     DASWBF010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210783"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VF874_09575"
     CDS             <1..182
                     /locus_tag="VF874_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S53"
                     /protein_id="HEX9176315.1"
                     /translation="NPLLYRIASGAPRPAFRDVTLGANAVDTASPGYDMITGLGPPNV
                     DNLVRNSLDIQGASR"
     gene            179..1369
                     /locus_tag="VF874_09580"
     CDS             179..1369
                     /locus_tag="VF874_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX9176316.1"
                     /translation="MSDPEVPTRPCPVCEHDVPAGAFCGFCGSHLISRRGEGPPWLRL
                     REFSAAPGEHVLRPSVTSSLFPHLHHRSQAPFRIGLAVLLVALVAFAVLRWQAPLVAV
                     SALGLPVLFHLYLVEAEAYKDFPRRALLLAAVLAVVLGIGWAWLTGAVIARSYDVAFG
                     AGMEFKQPLWQGVAIPVGGAVVMLIPTLLARLASSGTRESLDGFVIGAIAAMSFTAAA
                     TLTRLAPQFSTGLMTNRPVGVLIAEAGIRGVAMSLTAAAAGGMVGAALWFTKPDPAHQ
                     HRGQSLARPLPALGVVLTAYAVLGLVDFSPAPQTWQLILHLLIAALVTLALRVVLHLA
                     LLREAHDPITQEPLLCEHCAHVVPDMAFCPACGVATRASSRSSRIARRRLRPVQVDTP
                     PEEP"
     gene            1366..>1811
                     /locus_tag="VF874_09585"
     CDS             1366..>1811
                     /locus_tag="VF874_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176317.1"
                     /translation="MSTQAISGQSVWPGYALPAGSYTAPAIRRTSHSRVLMQLGVAVG
                     VLTLGVVIAFTLLTPPTPRYVCPPDCGQPPIGKPVERLPRFTSEDVAFSVSYPPEGTA
                     YDVTTKPNAVVAEFTGGDGGTLALFSEPAAGRGPAEIAKALLDEAYP"
BASE COUNT          234 a          629 c          633 g          315 t
ORIGIN      
        1 tgaacccgtt gctgtaccgg atcgcgtcgg gggcgccacg tccggcgttt cgcgacgtga
       61 cgctcggcgc gaacgccgtc gacaccgcct cacccggata cgacatgatc accgggctgg
      121 gcccgccaaa cgtcgacaac ctggttcgca actcgctcga catccaggga gcctcgcgat
      181 gagcgatcct gaggtgccga cgagaccgtg cccggtctgc gagcacgacg tcccggcggg
      241 agcgttctgc ggcttctgcg ggtcgcacct catctcacgg cgcggcgagg gaccaccgtg
      301 gctgcggcta cgggaattca gcgccgcgcc gggtgaacac gtgctgcgcc cgtcggtgac
      361 gagttcgctt tttccacact tgcaccaccg ttcgcaggca ccgttccgga tcgggctggc
      421 cgtgctgttg gtcgcactgg tcgcgttcgc ggtgctccga tggcaggcgc ctctggtcgc
      481 cgtcagcgcg ctcggattgc cggtgctgtt tcacctgtat ctggtggagg ccgaagccta
      541 caaagacttc ccgcgtcggg cgctgctgtt ggccgcggtg ctggccgttg tgctcggaat
      601 cggctgggca tggctgaccg gtgcggtcat cgcgcgcagc tacgacgtcg cgttcggggc
      661 aggcatggag ttcaaacagc cgctgtggca gggcgttgcc attcccgtcg gtggcgccgt
      721 cgtcatgctc atcccgacgc tgctcgcgcg gctggcgagc tccggcaccc gcgaatcgtt
      781 ggacggcttc gtgatcggcg ccatcgcggc gatgagcttc accgcggccg ccacgctgac
      841 gcgcctggct ccgcagttct ccaccgggct gatgaccaac agacccgtcg gcgtgctgat
      901 cgccgaggcc ggcatccgcg gtgtggcgat gtcactgacc gcggccgcgg cgggaggcat
      961 ggtgggcgcg gcgctgtggt tcaccaagcc ggaccccgca caccaacatc gcgggcagtc
     1021 actggcccgc ccgcttcccg cgctcggtgt cgtgctgacc gcctacgcgg tgctggggct
     1081 tgtcgatttc tcgccggctc cgcagacgtg gcagctcatc ctgcacctgc tcatcgcggc
     1141 gctggtgacg ctcgcgttgc gggtcgtgct gcacctggca ctgctgcgcg aagcgcacga
     1201 cccgatcacg caggaaccgc tgctgtgtga gcactgcgcg cacgtggttc cggacatggc
     1261 gttctgcccg gcctgcgggg tggcgacgcg cgcatcgtcg cgatcgtcgc ggatcgcgcg
     1321 acggcgtctc cgcccggttc aagtcgacac gccgccggag gagccgtgag cactcaggcg
     1381 atctccggtc agtccgtctg gcccgggtac gcgctgcccg cagggtcgta cacggcgccg
     1441 gcgatacgcc ggacatcgca cagtcgggtg ttgatgcagt tgggtgtcgc tgtcggcgtg
     1501 ctgaccctcg gtgtggttat cgccttcaca ctgctcaccc cgcccacccc gcggtacgtc
     1561 tgcccgccgg actgcggtca gccgccgatc gggaaaccgg tggagaggct gccccgcttc
     1621 acgtccgagg acgttgcgtt ctcggtctcc tacccgcctg agggcacggc ctacgacgtg
     1681 acgacgaagc cgaacgcggt ggtcgcggag ttcaccggcg gcgacggggg caccctggcg
     1741 ctgttcagcg agccggccgc cggtcgaggg cccgccgaga tcgcgaaggc gctgctggac
     1801 gaggcctacc c
//
LOCUS       DASWBF010000261         5496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1137829,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000261 DASWBF010000000
VERSION     DASWBF010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137829"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..4839
                     /locus_tag="VF874_09590"
     CDS             361..4839
                     /locus_tag="VF874_09590"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX9176318.1"
                     /translation="MAVTATVVTAGAAAGSALLGGTVVAGLGGSLARTAPDVSALARA
                     AAGMAIEAIGGPPARRGTGNGERCWIEVRGLGGKHASQIAADVLAAVRSTPGVQGAFL
                     NRSVARVVVTVEPGGPALAELCRIVADAERRTVGRVGQPPTSLPGDDAVLMGRMLAAA
                     AALVGLGFSVTGSVLRLRGLPDLVAVPTTMTDQVPRLRRQLEQRLGPEGTDLLFGLVN
                     AATASLTLSPTAAAAEAATRAMLAAEAWNGRLAWRRHEPGLASCPLPDDATGPSSDML
                     PPVDGPAERYADRAGLVGVGAAALVGVLSRNVDMAGAAALAATPKPLRGAREAFGCAA
                     SRGLIAGHGAMVLRPRALRTLDRVDAVMIDPRALYTDELTVSRVLGVQNSRRTRAWDA
                     VRTELGRNGLGPGWHRLADIAGVGGAGEALVSPVRDPLAAAVVTEARRSHPRVISLDD
                     DGLRSLAQGFDDLQPVGESIDESVAAAVDGLKDQGATVLLLTTADMQAAHRADITIGV
                     LRHRRPPPWGADVFVSDLSAAWRVLHALPAARAATDRGIRLSVSTSAIGALMLIPGVP
                     GRGPESVNAGVAAGLWAGFSAATKVFRDPVPEPDASHDWHALPVAEVQRLLPRPPDER
                     TGEASPRWPDMVPIRGLRGAAVSSWNLARDFAGEMRTNLSDPITPLLATGAVASALLG
                     SALDAALVGGVLLANATLSAEQQLHAERILRRLLAVQDPLARRRVGPLDERRHEKIPA
                     KRLRPGDIIEMHADEVIPADARLIDASNVEVDESTLTGESLPVCKQTEATPGAPLAER
                     ACMLYAGSTLVAGTALAVVTAVGSRTEVRRALAMAPTKSREIGLQRQLSRITRRALPF
                     SVVGGGLVGLLSVFRGTPLREAVGSAVALTVAAVPEGLPLVVTLAQLAAARRLTGECV
                     LIRNAHSVEALARLRVVCFDKTGTLSENRLQVKLVRPVAGFSPAEVLEAAATTIFVRP
                     GHRAHHATDDAIRGALDGGDESRTVTGPIQRDAFLPFQSGRPFAAALVGTRLTIKGAP
                     EVLASALSDDHVPRTRMIGEMAAQGLRVLAVAERRLTAEQAAAAVADPAVLESFCMSE
                     LSPVGLLGLADTPRASARALVAQLGDRGVEVRLITGDHPVTAAAVAKELGVEVAAEQV
                     ITGSQWETLSADERVEAVTSRRVFARMTPEHKVDIVQTLERIGWVTAMVGDGANDAAA
                     IRAASVGIGVAARGSDPARTAADVVLLDGRIEAMIDALNEGQQLWRRVQSAVSMLLGG
                     NTGELCFALLTSLLTGRSVLNARQMLLVNMLTDALPAAALAVSMQTSTAEVDRDEAAM
                     WRAIGIRGASTTVGATLAWLMASATGTRRRAATVALIGLVGTQLAQTLADSHGRLVVL
                     TTVGSFAVLVGVVSTPGVSQVFGCTPVGPLGWGQAFLATAVAAALSALAPELLYRATE
                     AVRRRLAATADGDDESVVDDHDAGLHEDRVDIAQGRRQQTNTGHDQGIMADTTQKL"
BASE COUNT          793 a         1790 c         2009 g          904 t
ORIGIN      
        1 ccaggaaccc catcctcgag gttagctcgt ccgtaagata atcaacctca tagcgcatgg
       61 gttgtcgcct cgtttcggcc ggtgaggcag tcgagacagc caggacgggg tatgccgcgt
      121 acgccgtggg cgacgtcgta acggccctga gcctgttcgc accgcacctg tcgatttttg
      181 gcgaggaggg gccgcggacg ccgatgtggt tagcccaata ttcatgtgct ggcaaccggc
      241 ttctgttaag ccatcgcatc attcccagct gcgctgggta tggtcggcag agtgatcggg
      301 cggcgtgttc tgccggggtt gctgacggcg cccctgcgcg ccgccgcggc aggcagccgg
      361 atggctgtga cggcgaccgt tgtgacggct ggtgccgctg cgggcagcgc gctgttgggc
      421 ggcacggtcg tggccggctt gggtggctcg ttggcccgta ctgcaccgga tgtttcggct
      481 ttggcccgtg ccgccgcggg catggcgatc gaggcgatcg ggggtccacc cgcgcgtcgc
      541 ggcaccggca atggtgagcg ttgttggatc gaggtgcggg ggcttggcgg gaagcacgcc
      601 tcgcagatcg ccgcggatgt ccttgccgcg gtgcgatcga cgcccggggt ccagggtgcg
      661 tttttgaacc ggtccgtcgc gcgagtggtg gtgacggtcg agcccggtgg gccggcgttg
      721 gcggaactgt gccgaatcgt cgccgatgct gaacggcgaa ccgtaggccg cgtcgggcag
      781 ccgccgacga gtctgcccgg tgacgacgcg gttctgatgg gtaggatgct ggcggccgcg
      841 gcagcccttg tgggccttgg gttttcggtg actggcagcg tgctgaggct gcggggattg
      901 ccagacctgg ttgccgtgcc gacgacgatg accgaccaag ttccgcggct acgccgccag
      961 ctggagcagc gtctcggacc ggagggcacc gatctgctgt ttggcttggt gaatgcggcg
     1021 acggcatcgt tgacgctgtc gccgacggcg gcggcggccg aggccgcgac tcgggcgatg
     1081 cttgccgccg aggcgtggaa tggtcggctg gcgtggcgcc ggcatgagcc gggcctggcc
     1141 agctgcccgt tgcccgatga cgcgacaggc ccttcgagtg acatgctgcc tccggtcgac
     1201 ggacccgcgg aacgctacgc cgaccgggcc gggctcgtcg gcgtcggcgc cgccgctctg
     1261 gtcggtgtgc tgtcgcgcaa tgtcgacatg gctggtgcgg ccgcgttggc ggcgacaccc
     1321 aagccgcttc gcggtgcccg ggaagctttc gggtgtgctg cgagccgcgg cctgatcgcc
     1381 ggccacggcg ctatggtgtt gcgcccccgg gctttgcgga cgctcgaccg ggttgatgcg
     1441 gtcatgatcg acccgcgggc gctctacacc gacgagttga cggtcagtcg ggttctgggc
     1501 gttcagaatt cgcggcggac tcgagcgtgg gacgctgtcc gcactgagct gggccgaaat
     1561 ggcctgggtc ccggctggca caggcttgct gatattgccg gcgtcggcgg ggcgggcgag
     1621 gccctggtca gcccggttcg cgatccgctc gccgcggcgg tggtcaccga ggcacggcgg
     1681 tcacacccac gcgtcatctc gcttgacgac gacggattgc gttcgctggc gcaaggattc
     1741 gatgatctgc aaccggtggg tgaatcgatc gatgagtcgg tggcagcggc cgtcgacggc
     1801 ctcaaggatc aaggggccac ggtgttgctg ctgaccaccg ccgacatgca ggcggcgcat
     1861 cgcgccgaca tcaccatcgg cgtgttgcgt caccggcgtc cgccgccgtg gggtgccgac
     1921 gtgttcgtct ccgacctcag cgccgcctgg cgcgtgctgc atgccctgcc ggccgcccgc
     1981 gccgccaccg accgcggcat ccgactgtcg gtctccacgt cggcgatcgg cgcgctcatg
     2041 ttgatccccg gcgtgcccgg acgcggcccc gaatcggtca acgcgggggt ggccgctggg
     2101 ttgtgggccg gattctcggc ggccacaaag gtgttccgcg atccggtgcc cgagccggac
     2161 gcaagccacg actggcacgc gttgccggtc gcggaggtac agcggctgct gccccggcca
     2221 cccgacgaac gcaccggcga agcgtctccg cggtggcccg acatggtgcc gatacgcggg
     2281 ttgcgtgggg cggcggtgtc gtcctggaac ctcgcccgcg atttcgccgg cgagatgcgg
     2341 accaaccttt ctgacccgat caccccgctg ctggccaccg gcgcggtggc cagcgcgctg
     2401 ctgggctcgg cgctggacgc ggcgctggtg ggcggggtct tgctggccaa cgccacgctg
     2461 tcggccgagc agcagctgca cgccgaacgc atcctgcgtc ggctgctcgc cgtgcaggat
     2521 cccttggccc gacggcgcgt cggtccactc gacgagcgcc gacacgagaa gattccggcc
     2581 aagcggctgc gacccggaga cattatcgag atgcacgccg acgaggtcat ccccgccgac
     2641 gcccggctga tcgacgcgtc gaacgtcgag gtcgacgaat ccacgctgac cggcgaatcg
     2701 ctgccggtgt gcaaacagac cgaagccacg ccgggggcgc cgctggccga acgggcgtgc
     2761 atgctttacg ccggcagcac cctggtcgcc ggtacggcgc tggccgtcgt gaccgcggtg
     2821 ggttcgcgca cagaggtgcg tcgggcgttg gcgatggcac cgaccaagtc gcgcgagatc
     2881 gggttgcagc gacaattgag ccgcatcacc cggcgcgcgt tgccgttcag cgtggtgggt
     2941 ggcgggctgg tcgggctgct cagcgtgttc cgcggcacac cgttgcggga ggcggtcggc
     3001 agcgcggtgg cgctgaccgt tgccgccgtt cccgaaggac tgccgctggt ggtgacgctc
     3061 gcccagctgg ccgccgcgcg ccggctcacc ggcgaatgcg tgctgatccg caacgcccac
     3121 tcggtggagg cgctggcccg gctgcgggtg gtgtgcttcg acaagaccgg aacgttgagt
     3181 gagaaccggc tgcaggtcaa attggtgcgg ccagtggccg ggtttagccc agctgaggtg
     3241 ctcgaagcgg cggccaccac gatctttgtc cggcccgggc accgggccca ccacgccacc
     3301 gacgacgcga tccgcggggc tctcgacggc ggcgacgaat cgcgaaccgt cacaggcccg
     3361 atccagcgcg acgctttcct gccgttccag tcgggtcgcc cttttgcggc ggcgctggtg
     3421 ggtacgcggc tcacgatcaa aggtgcgccc gaggtgctcg cgtcggcgct cagcgacgac
     3481 catgtgcctc ggacgcggat gatcggcgag atggcggccc aggggctgcg cgtgctggcg
     3541 gtggccgagc gccggttgac cgccgagcag gccgccgcgg cggtcgccga cccggcggtg
     3601 ctggaaagct tctgcatgtc cgaactcagc ccggtcggct tgctcgggct ggccgacact
     3661 ccgcgagcca gcgcccgcgc actggtggcg caactcggcg accgcggcgt cgaggtccgg
     3721 ttgatcaccg gggatcaccc ggtcaccgcg gccgccgtcg ccaaggaact gggcgtggag
     3781 gtcgccgccg agcaagtgat caccggcagc caatgggaaa cgctgtccgc cgacgaacgg
     3841 gtcgaagccg tcacgtcgcg tcgggtgttc gcccggatga cacccgagca caaggtcgac
     3901 atcgtgcaga cgctggagcg gatcgggtgg gtcacggcga tggtcggcga cggcgccaac
     3961 gacgccgcag cgatccgggc ggccagtgtc ggcatcggcg tggccgcccg cggcagcgac
     4021 ccggcccgca ccgcggccga cgtggtactt ctcgacgggc ggatcgaggc gatgatcgac
     4081 gcgctgaacg agggccaaca actatggcgg cgggtgcagt ccgcggtgtc aatgctgctg
     4141 ggcggcaaca ccggcgagct gtgtttcgca ctgctcacca gcctgctcac cggccgctca
     4201 gtgctcaacg cccgccagat gctgctggtc aacatgctca ccgacgcgct gcccgcggcc
     4261 gcactggccg tcagcatgca gacaagcacc gccgaagtgg accgggacga ggcggcgatg
     4321 tggcgggcga tcggtatccg gggtgcctcg accaccgtcg gcgccacgct ggcctggctg
     4381 atggccagcg cgacaggcac tcggcgccgc gccgcgacgg tcgcgctgat cggcctggtc
     4441 ggcacacaac tggcgcagac gctcgcagac tcacacggcc ggctggtggt cctcaccacg
     4501 gtcgggtcgt tcgcggtgct ggttggtgtc gtcagcacgc cgggggtgag ccaggtgttc
     4561 ggctgcaccc cggtaggccc gctgggctgg ggccaggcct ttcttgcgac tgcggtggcg
     4621 gcagcgctct ccgcgctggc ccccgaactc ctatatcgcg caacggaagc ggtgcggcga
     4681 cggctcgcgg ccaccgccga tggagacgac gaatcagtcg tcgacgacca tgatgccggc
     4741 ctgcatgagg accgcgtaga tatcgcgcag gggcggcgtc agcagacgaa caccggccat
     4801 gatcaaggga tcatggccga caccacgcag aaactctgac acgtgaccca accaacccgt
     4861 tcggtgtgac gaggcgggat cgcacagttg aacaggacac gaaatattga agacgccacg
     4921 atcgttcctt gcttcccaac gcaatgcgtc ccggacggtt caggttcagc aggcggagaa
     4981 gttctcactc cgtctgcccg tggtgggcca ggtgtcggtg ctgccgcccg atcagctcgc
     5041 cttttacgga ctccggcctg gagtgagccg tatgcccgtc tctgggggtg agtggtgtcg
     5101 cgggtcttct aaaaggcgtc gcctcgaata tcccccgaca ccgtgtgcac ggttggcggt
     5161 gcttccagcc ccatcatcgc tgcgcccgga atctggtacg gtagtactac taattttgcc
     5221 gtcggtctgc cgtcaacccc cgcggatcag cgcgaacaac ggcgaactgt cacgaatgtg
     5281 tatgcgcagg tggcgggcat gcacggatga atgcgaaccc agccgaactg ggcttcaatc
     5341 gccagggtcg tcagttcaat caacagctat atcgaaatgg cgttcaatca gatggtcggt
     5401 gtcacgaggg tggctgcgga gtagatgcgt gcgtcccagc cggccaattg aaggcatgtt
     5461 gacgccgacg tggccggggt atcgggcagg ttgcct
//
LOCUS       DASWBF010000262         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1072831,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000262 DASWBF010000000
VERSION     DASWBF010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072831"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VF874_09595"
     CDS             complement(<1..701)
                     /locus_tag="VF874_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX9176319.1"
                     /translation="MREHPSTIDALLRLRAAHDGAKPAVIDPVTRITYRELDATTRDQ
                     AAVFVDAGVRKGTRVGLIMPNSVRWVQIALALTRIGAVMVPLSTLLQPRELLAQLHTA
                     SVQVLISVQEFRGHRYLDDLRSVDTKLPALQHVWPADRLAQATADEAAARIADSLTTT
                     VTPSDPMVIMFTSGSSGPPKGVVHSHGNALGAVASGLAARCIDGDTRLYLPMPLFWVG
                     GFGSGILSALLAGATL"
     gene            complement(694..>1570)
                     /locus_tag="VF874_09600"
     CDS             complement(694..>1570)
                     /locus_tag="VF874_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX9176320.1"
                     /translation="GGLGFGLLATLVAGSTLVCSNAADAGDTLDLLEAEKPTMTNGFA
                     ASIAHLARHPSFEARDLSSMRRGNLYPIMAPDARPADPELRHNMLGLTEAGSVVLLSG
                     DETDQPQHRRGSFGKPAPGFQTKVVDLDSGNPVDVGDVGELCLRGPYVMQRYYKRSRE
                     ECFDADRWFHTGDLVRTDGDGFYYFVGRRGSMIKTAGANVSPPEVEKAIAKVTGGKVA
                     YVVGIPHPERGQQVAAVVVVGDGEQFDESALRHELKSQLSAYKIPTRFVAVSEVPLLS
                     SGKVDMQKLMTVFDA"
BASE COUNT          267 a          521 c          519 g          263 t
ORIGIN      
        1 agcgttgcgc cggctagcag cgccgaaagg attccgctgc cgaagccgcc gacccagaac
       61 aacggcatcg gcaggtacag ccgggtgtcg ccgtcgatgc aacgcgccgc gaggcccgac
      121 gcgaccgcgc ccaacgcatt gccgtgtgag tgcacgaccc ccttcggggg tccgctgctg
      181 cccgacgtga acatgatgac catcgggtcg ctgggagtga cggtggtcgt gagcgaatca
      241 gcgatccggg cggccgcctc gtcagctgtg gcctgcgcta gacggtcggc tggccaaaca
      301 tgctgcaacg ctggcagttt ggtatccact gaccgcaggt cgtcgagata acggtgaccg
      361 cggaactcct gcaccgagat caggacctgc accgaggccg tgtgcagctg cgccagcagc
      421 tctcggggtt gcagtagcgt gctcagcggc accatcacag caccgatccg tgtcagcgcg
      481 agggcgatct gcacccatcg cacgctgttg ggcatgatca gcccgacacg ggtgcccttg
      541 cgcacgccgg cgtcgacgaa gactgcggcc tggtcccggg tcgttgcatc cagctcgcgg
      601 tatgtgattc gcgtcaccgg gtcgatcacc gctggtttgg ctccgtcatg ggcggcacga
      661 agtcgcagca gtgcatcgat ggtggaggga tgctcacgca tcgaacaccg tcatgagctt
      721 ctgcatgtcc accttgccgc tcgacagcag cggcacctcg gacaccgcca cgaaccgcgt
      781 cgggatcttg tacgccgaca gctgcgactt cagctcgtgc cgaagcgccg actcgtcgaa
      841 ctgctcgccg tcgcctacga cgacgaccgc ggcgacttgc tggccgcgtt ccgggtgagg
      901 aataccgacc acgtacgcca ctttgccgcc ggtgaccttg gcaatggcct tctccacttc
      961 tggcggagac acattggcgc cggccgtctt gatcatcgaa ccgcgcctgc cgacgaaata
     1021 gtagaacccg tcgccgtcgg tgcggacaag atcccccgtg tggaaccacc tgtcggcgtc
     1081 gaagcactcc tctcggctgc gcttgtaata gcgctgcatc acatacggtc cgcggaggca
     1141 caactcgccg acatcgccga cgtcgacggg attcccggag tccaggtcga cgactttggt
     1201 ctggaaaccg ggcgccggct tgccgaacga gccgcgccgg tgttgcggtt ggtcggtttc
     1261 gtcgccgctg agcagcacga cgctgcccgc ttcggtcagc cccagcatgt tgtggcgcaa
     1321 ctcgggatcg gcaggccggg cgtcaggggc catgatcggg tagaggttgc cgcgccgcat
     1381 cgacgacagg tcgcgggcct cgaaactcgg gtgccgggca aggtgcgcga tcgaggcggc
     1441 gaaaccgttg gtcatcgttg gcttctcggc ttccagcaga tcaagagtgt cgccggcgtc
     1501 ggcagcgttc gagcacacca gtgtcgagcc ggcgaccagc gtggcgagca gaccgaaccc
     1561 caggccgccg
//
LOCUS       DASWBF010000263        13555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_947974,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000263 DASWBF010000000
VERSION     DASWBF010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13555
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947974"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="VF874_09605"
     CDS             complement(<1..1511)
                     /locus_tag="VF874_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9176321.1"
                     /translation="MSTYLRLPVFRDHPIAALAVICLSVFVISVDATIVNVALPTLSR
                     ELDADTAQLQWIVDAYTLVMSGLLLSVGSLSDRYGRRGWLSAGLAVFAITSAVAAQVN
                     SAEALIAARAAMGVGAAVIFPTTLGLITNIFTDPAPRGRAIGLWAAMVGVGVAVGPIT
                     GGWLLEHFSWGSIFLVNVPVAAVAIAGGILFVPTSRDPAAPRVDVGGLILSSIGVTAL
                     VYTVIEAPNWGWTSTRTTAGFGLAIVVLTGFALWERRSSHPMLDVSVFANRRFSGGSL
                     AVTAGFLTLFGFIFVITQYFQFVKSYSAFETGVRLLPVAASIAAASILGPRMVERFGT
                     TAVVVAGLAVFAAGLGWASTVDGSTPYTMIATQMVLLGAGLGLTTAPATEAIMGSLRP
                     DKAGVGSAINDTTRELGGTLGVAIVGSVFASVYSGRLAAESALTTLPGDVRATMGRSM
                     AAAYQVIAQLPAGRVGGVRDAVNDAFLNGLQVGSLVCAGIALGAAVVVAWLLPA"
     gene            1590..2258
                     /locus_tag="VF874_09610"
     CDS             1590..2258
                     /locus_tag="VF874_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9176322.1"
                     /translation="MTARRNYNQNCPIARGLDVLGERWTLLILRELVGGPRRYGDLRA
                     ELPGIASNLLADRLRELEQAGLVDRSELPPPVARTVYTLSDLGWQKVLPVLKAIAMFG
                     LDRVEPTESALTPLNGFLVGALLAFEPARAVGLSASYRVEIDGRPFEFAVRREGLTAA
                     RRPPAVTLSATAADLITARMGSSLAERKSALRRVKFDGDDDAIAAMRTAFQLAGDPGL
                     SIVA"
     gene            2476..2751
                     /locus_tag="VF874_09615"
     CDS             2476..2751
                     /locus_tag="VF874_09615"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9176323.1"
                     /translation="MRTDGETLWWPDYPGNNMFNSFGNLAADPTAALLFVDFRTGHTL
                     HLSGTATVRWNISGDPDGDGHTGRGAHFVPTRVAAGRLLPAREIGYD"
     gene            complement(2742..3152)
                     /locus_tag="VF874_09620"
     CDS             complement(2742..3152)
                     /locus_tag="VF874_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176324.1"
                     /translation="MRPRSAYWGYPTTHTIPGSIPDNLDALANALVKRATPPREPADR
                     WARYLEAVRAKGIEQAKQASSQATDHVAATDLFRELHDALPDDAICVDEIVAQMDQMI
                     QFLYEHKPFTQYRGWAGALGMSLGTALGVKLCQS"
     gene            3516..4820
                     /locus_tag="VF874_09625"
     CDS             3516..4820
                     /locus_tag="VF874_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX9176325.1"
                     /translation="MTTSPQPAGWYPDPSGTPAQIVAPHPKDDLFYSYAGSTPLASIA
                     PGTVLATRSIAYHILGVPTSLKTTQLLYRSTSQTGNPTVNVTSVIQPLSQRDKTKVVS
                     YQSAYDSLNRNDEPSYAISGGLTLGGIVPNVELAVFGTFLALGYTVIVPDTEGQRADF
                     AAGPEYGMNTLDSIRAAFNSSHVGLPADAKVAMLGYSGGAIATEWAAELAPTYAPDVN
                     GRMIGAAMGGVLVHPAHNLHYVEGTYFWAGVMPMALVGIARAFEIDFTPYLSARGVKL
                     YHEVMTASIVNVLGQYPYWGLTWADLVSPAYPTPERLPPYVRQANKLIMGTGGTPTIP
                     LFIGQGANGDLEWTPGDKPGIGAGDGVMIAGDVRTLARDYCSKGTTVYYEQYDALSHI
                     TSIGVWLPNSIAWITQRFAGVPAPQNCSSIPPGNSLDPIPVP"
     gene            4862..5485
                     /locus_tag="VF874_09630"
     CDS             4862..5485
                     /locus_tag="VF874_09630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX9176326.1"
                     /translation="MTYAQGTTGLVVIDPYNDFISEGGKVWDRLKGVAEANRCVPHMK
                     QVLDAARQVDIRVFYALHRRYRPGDYETWKYVAPIQKAAWERKTFEFGTWGGELRDEF
                     QPRAGDIVAQEHWCSSGFANTDLDLLLKKHGIHELIAVGLIAHTCVEATVRYGAELGY
                     EVTVVKDATASYSEDHMHAALEVNIPNYATAIVTADEIADALRAPEP"
     gene            complement(5531..6028)
                     /locus_tag="VF874_09635"
     CDS             complement(5531..6028)
                     /locus_tag="VF874_09635"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9176327.1"
                     /translation="MRQSTAAVGSAAFFVVAPGTVVGLIPWLITGWKVSGSLWQVAVG
                     APLIIAGLIPLALAFVEFAKAGGTPMPVAPTERLVVTGFNRYVRNPMYVGLLVAILGQ
                     ALLFGSVGLLVYAAVVWVVTASFVRWYEEPTLSRRYGNEYEAYRRNVRAWIPRLRPWN
                     SGGDQ"
     gene            complement(6040..6441)
                     /locus_tag="VF874_09640"
     CDS             complement(6040..6441)
                     /locus_tag="VF874_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9176328.1"
                     /translation="MPAPRWVARANKVGLNRLTAFIAPWAPGWAVVVHRGRRSGRTFR
                     TPLWAFRRRGGFVIALTYGSDADWVRNVVAAGGCELETRRRRYRVAAPRVYRDENATD
                     MPAFIRFMLRRVIKAPEFLSLDVVDQPVKAA"
     gene            6531..7136
                     /locus_tag="VF874_09645"
     CDS             6531..7136
                     /locus_tag="VF874_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9176329.1"
                     /translation="MPPLGRRPGKTDTRNVLLQNARRMFAEGGYNHTSVRDIAAAAGV
                     DPAMIRHYFGSKAQLFRATMGWPFDPGQLAAQITSGDRSEIGARLTQVFFGAWEQPDS
                     RAPLLAILRGAATHEESATLVRQFIQTQLYPQIAAALEDPKAELGVDLAMAQLLGIAY
                     LRHILCVEPIASTPIDELVARVAPVISAHLDLSTGATLNEQ"
     gene            7509..8809
                     /locus_tag="VF874_09650"
                     /pseudo
     CDS             7509..8809
                     /locus_tag="VF874_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
     gene            8824..9606
                     /locus_tag="VF874_09655"
     CDS             8824..9606
                     /locus_tag="VF874_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9176330.1"
                     /translation="MPKRTEYAQGTPNWVDLQTTDQDAAKQFYASLLGWSYDDQPIPQ
                     GGVYSMATVNGETVAAIAPMPPGAPEGMPPVWNTYIAVDDVDAAVGKVEPAGGQVLMP
                     GFDIGDAGRMAFIADPTGAAVALWQANQHIGATLVNETGAVIWNELITDKPESALAFY
                     EAVVGLEHSMMEMAPGQNYTLLKAGGSDVGGCMEPPMPGVPNHWQVYFAVDDTDATAA
                     KASAEGGQIAVEPFDIPSVGRSAVLTDPQGAMFSVLQPTPQQ"
     gene            complement(9669..9846)
                     /locus_tag="VF874_09660"
                     /pseudo
     CDS             complement(9669..9846)
                     /locus_tag="VF874_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065511.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
     gene            complement(9865..10509)
                     /locus_tag="VF874_09665"
     CDS             complement(9865..10509)
                     /locus_tag="VF874_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9176331.1"
                     /translation="MARREKIPEISSEQRDTYHHGDLKRALTEAALGLVKEKGPKGFT
                     LREVARRAGVSAAAPYRHFSDKAQLLAAAATQGFVQLHEALNAAAENTDLTRQVLDMG
                     RAYVRWALTHPDYYQVMFGAELDKSDKPELLAAGARAFGDLLDVTVRCQEANLLPAGD
                     PRDIAGPTWSLLHGIASLTIGSDFQHVGIREDPEMLAERAVRALLFPRTTKSGA"
     gene            10570..11397
                     /locus_tag="VF874_09670"
     CDS             10570..11397
                     /locus_tag="VF874_09670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9176332.1"
                     /translation="MSKKREVSAGWAVVTGASSGLGALFAEKLAQRGLPLVLSGRDEA
                     RLAEVRQRVQRLAPGVDVELVVGDLGIHAGVDALVAVLDGRVIDVLVNNAGFGTYGRL
                     PEIEPDRDHELIAVNVDALVRLTHAVLPGMLARGHGRILNVASTIAFQPAPYQATYGA
                     SKAFVLSFSQALWAETRGSGVTVTALCPGPTRTGFVDALDADVSGTAIYRRLASPETV
                     VAAGLRALDRGRPVVVPGLRNRVMATGASLSPGWVGALIGGRMLRPARNSRAHVASQ"
     gene            11394..12314
                     /locus_tag="VF874_09675"
     CDS             11394..12314
                     /locus_tag="VF874_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176333.1"
                     /translation="MITGSGVEAADDAAQMVAAVRDDPAGRIRLAADAYAFHRGGRRY
                     RPYRRAVVAFMRWQQARSVLNSLDDDVPGSPWWRAVNEDLLRDTLEARLLVERGGGAP
                     SRPTVARWVSFFDAPSAQSWYGAHNASVVAGYLAHSSLAALEVPAERFFMNVVLVRVL
                     YAHALVLDGDLALGRLSFLARLIGHPRSRGPQALLAMKDVLPEQYPIQEARVEELIES
                     ENRLGRMVDYGVIGARVDALYASSARALGEPLLLDLIIDGAPAYAWPADQRHVWKPPS
                     QRLFTSLIEFLTRPRNSRVQLAGMAVGIAG"
     gene            12399..13148
                     /locus_tag="VF874_09680"
     CDS             12399..13148
                     /locus_tag="VF874_09680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX9176334.1"
                     /translation="MTTSKPLAGRRALVTGGSRGIGAEIVRRLADDGAAVAFTYGASA
                     ADADKLATEVAARGGTAIAIQADSADPEQVANAVDATVSRLGGLDILVNNAGVAYIGG
                     IESFPLEQFDRLVAINVRGVFAAIQRAVPHLGDNGRIITIGSINADRVPVPGLSVYAM
                     TKAAVAGLTRGLARELGPRGITVNNIQPGPIATDMNPEEGEFADAARQVIALGRYGRP
                     RDVASVVSYLAGPESAFITGVNWDVDGGFAA"
     gene            13340..13555
                     /locus_tag="VF874_09685"
                     /pseudo
     CDS             13340..13555
                     /locus_tag="VF874_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740220.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
BASE COUNT         2399 a         4401 c         4482 g         2273 t
ORIGIN      
        1 gcaggcagca gccacgcgac gacgaccgct gcgcccaatg cgattccggc gcagaccaac
       61 gacccgacct gaaggccgtt gaggaatgcg tcgttcacgg catcgcggac gccgccgacg
      121 cgcccggccg gcaattgcgc gatgacctgg tacgccgcgg ccatcgaccg tcccatcgtg
      181 gcccggacgt ccccgggcag tgtggtgaga gccgactcgg cggcaagtcg accggagtac
      241 acggatgcga agacgctgcc gacgattgcg acgcccagcg tgccgcccag ttcgcgggtg
      301 gtgtcattga tcgccgaccc gacaccggcc ttgtccggtc ggagtgagcc catgattgcc
      361 tcggtggccg gtgccgtcgt cagcccgagg ccggcaccga gaaggaccat ctgcgtggcg
      421 atcatggtgt agggggtgct cccgtcgacg gtggatgccc aaccgaggcc ggccgcgaag
      481 accgccaggc cggcgacgac gacggccgtg gtgccgaatc tctcaaccat gcgcgggccg
      541 aggatgctcg ctgcggcgat cgaggccgcg accggcagca accgcacacc cgtctcgaat
      601 gcgctgtagc tcttgacgaa ttggaagtac tgggtgatga cgaagatgaa cccgaacagc
      661 gtcaagaatc cggccgtaac tgccaggctg ccaccggaga accgccgatt cgcgaacacc
      721 gacacgtcga gcatcggatg cgagctgcgc cgctcccaca gtgcgaagcc ggtgagaacg
      781 acgatggcca acccgaagcc agcggtggtg cgcgtgctag tccatcccca attcggggcc
      841 tcgatgaccg tgtagaccag ggcggtcacc ccgatcgacg acagaatcag cccgccgacg
      901 tccacgcgcg gcgccgccgg gtcgcgcgac gtcgggacga agagaatccc tccggcgatc
      961 gcgaccgcgg cgacggggac gttgaccagg aagatcgaac cccacgagaa gtgctcgagc
     1021 agccagccac cggtgatcgg cccgaccgcg acaccgacgc ccaccatggc ggcccacagc
     1081 ccgatcgccc ggccgcgcgg cgccgggtcg gtgaagatgt tggtgatcag ccccaatgtt
     1141 gtcgggaaga tcaccgcggc gccgacgccc atcgcggcgc gcgccgcgat cagcgcttcg
     1201 gccgagttca cctgcgccgc aacggcagag gtgatcgcaa atacagccag cccggcactg
     1261 agccagccac gtcggccgta gcggtcgctc aggctgccca ccgacaacag cagcccggac
     1321 atgacaaggg tgtaggcgtc aacgatccat tgcagctgcg cggtgtcggc gtcgagttcg
     1381 cgcgacaacg tgggcagtgc gacgttgacg atcgtggcgt cgacgctgat gacgaagacg
     1441 ctcagacaga tgacggcaag cgcggcgatc ggatgatcgc ggaatacagg gaggcgaaga
     1501 taggtactca taggcatgaa gataaaccaa ggaaacaaac aaatcgagta acaagttgaa
     1561 aattagatct gagtggtata gttgctggtg tgaccgcacg gaggaactac aaccagaact
     1621 gccccatcgc gcgcggcctc gacgtcctcg gcgaacggtg gaccctccta atcctccgcg
     1681 agctggtcgg aggtccgcgc cgctacggcg atctgcgcgc cgaactaccg ggcatagcga
     1741 gcaacctgct ggcagaccgg cttcgcgaac tcgaacaggc cggtctcgtc gaccgctccg
     1801 aactaccgcc accggttgcg cgcaccgtgt ataccctcag cgacctgggc tggcagaaag
     1861 tgctgccggt gctcaaggcg atcgccatgt tcggcctcga tcgggtcgag cccaccgaga
     1921 gcgccctcac gccgttgaac ggctttcttg tcggcgcgct gttggccttc gaaccggctc
     1981 gcgccgtcgg cctgagcgcc tcgtatcgtg tcgaaatcga tggccgccca ttcgaattcg
     2041 cggtccgtcg cgagggtctg accgccgcca ggcggccgcc cgcggtgaca ctgtccgcga
     2101 ccgcagcaga tctgatcaca gcccgtatgg ggtccagctt ggccgaacgc aagtcggcgc
     2161 tgcggcgggt gaagttcgac ggtgacgacg acgctatcgc cgcgatgcgc acggcttttc
     2221 agctcgcagg agacccgggg ctctcgatcg tggcttagcg gacatgaaga gggccggcga
     2281 acgcggcgcc ggcgcggtcc gcgccggcgc cggggtttgc gatcaagaca agcggaggcg
     2341 cgctcgaggc ggccggtgag gtgttcgact ccgccccgta cggtcggggc cggtggccaa
     2401 ggggtcagcg ctggccgagt cgctgcgcct gagcgcggca acggtgcttc gcgccgcggc
     2461 ggtccgctcg gattcgtgcg cacagacggc gaaaccctgt ggtggcccga ctatccgggc
     2521 aacaacatgt tcaacagctt cggcaacctg gctgccgacc cgacggcggc gttgctgttc
     2581 gtcgacttcc gtaccggaca tacgctgcac ctgtccggca cagcgaccgt gcggtggaac
     2641 atctcgggtg atcccgacgg cgacggccac accggacgtg gtgcgcactt tgtgcccacg
     2701 cgcgtggccg ccgggcggct tctccctgca cgcgagatcg gctacgactg acatagcttg
     2761 acgccgagtg cggtcccgag gctcatgccc agggcgccgg cccacccgcg atattgcgtg
     2821 aaaggcttgt gttcatagag gaattggatc atctgatcca tctgggcaac gatctcgtcg
     2881 acacaaatcg cgtcatcggg caaagcgtcg tgcagttcgc ggaacaggtc ggtcgccgca
     2941 acgtgatccg ttgcctggga cgacgcctgc ttggcctgct cgatgccctt ggcgcgcacg
     3001 gcttccaagt aacgggccca ccggtccgcg ggttcgcgag gcggcgtcgc tcgtttcacc
     3061 aaagcgttcg caagcgcatc gagattgtcg ggaatgcttc ctgggatggt atgggtggtc
     3121 gggtatcccc agtacgcgct gcgcgggcgc atgggggtct tcctcgagac ggatgacggc
     3181 gcatccttcg cgcaatgctg gcagcggcgg atgccacggc gcgttgcagc ccgccagcag
     3241 gatggcgtcg ggttcaccaa gcacaggctc cacgggaccc gcgccgtaca acggatggga
     3301 ccgagggaag ttgtgatagc agggcagcga tgggggtgcg aaaacgcatt cggcgccctg
     3361 ggattccaac tgctccagca ggacttcggc gccgttcttg tcggaattag cagtcggcat
     3421 gggtcatcct aggccgccac ggtgacgctc cggggctgtt tgtcccaggt ggcgggtctg
     3481 cctggcaaca tggtgcccac gattgagggg agcgaatgac tacatcaccg caaccggcag
     3541 gctggtaccc cgacccgagt ggcacgcccg ctcagattgt ggcgccacat ccaaaggatg
     3601 atctgttcta ctcgtacgcc ggcagcactc cgctggcgag cattgcaccg ggaacggtgc
     3661 tggcgactcg cagcattgct taccacatat tgggggtacc gacgtcgctg aagaccactc
     3721 agctgcttta taggtcgacc agccagaccg gcaacccgac cgtgaacgtc acctcggtca
     3781 ttcagccgct gagccagcgc gacaagacga aggtggtctc gtatcagtcc gcctacgact
     3841 cgctcaaccg caacgacgaa ccgtcatatg cgatttcggg cggactcaca ctcggcggga
     3901 ttgtccccaa cgtcgagctg gcggtgttcg gtacgttcct cgccctgggg tacacggtga
     3961 tcgtgcccga caccgagggt cagcgggcgg acttcgccgc cggccccgaa tacggaatga
     4021 acacactcga ctcgatccgc gctgcgttca actcgtcgca cgtcgggctg cccgcggatg
     4081 cgaaggttgc gatgctcggc tactccggcg gtgcgatcgc caccgagtgg gccgccgagc
     4141 tggctccgac gtacgcgccg gacgtcaacg ggcgcatgat cggtgcggcg atgggcggcg
     4201 tgcttgtcca cccggcacac aacctgcact acgtcgaagg cacctacttc tgggccggcg
     4261 tcatgcccat ggcgctggtc ggcatagcgc gcgcgttcga gatcgacttc acgccctatc
     4321 tcagcgcccg cggcgtgaag ctctaccacg aggtgatgac ggcgtcgatc gtgaacgtcc
     4381 tcggccagta tccgtattgg ggtttgacgt gggctgacct ggtcagcccc gcgtacccga
     4441 ctcccgagcg cctgccgccc tacgtcaggc aggccaacaa gctgatcatg gggaccggcg
     4501 gtacgccgac gatccccctg ttcatcggcc agggcgccaa cggtgatctc gagtggacgc
     4561 cgggcgacaa gccgggcatc ggcgctggcg acggcgtgat gattgccggt gatgtgcgca
     4621 cgcttgcccg ggactactgc tcgaagggca cgacggtcta ctacgagcaa tacgacgcgc
     4681 taagccacat cacgagcatc ggggtctggc tgccgaactc gatcgcctgg atcacgcaac
     4741 gcttcgccgg cgtgccggcg ccgcagaact gctcgtcgat cccgccgggc aattcgctcg
     4801 accccattcc ggttccgtag agcggatctg cagtcaacgc cgaccggagg aagatgaccc
     4861 cgtgacatat gcccaaggca ccaccggact tgtggtgatc gatccgtaca acgatttcat
     4921 ctccgagggt ggcaaggtgt gggaccgctt gaagggcgtg gccgaagcca accgctgtgt
     4981 tcctcacatg aagcaggttc tcgatgccgc gcggcaggtg gacatccgtg tgttctacgc
     5041 gctgcaccgt cgatatcggc ccggtgacta cgagacctgg aaatacgttg cacccattca
     5101 gaaggccgcg tgggagcgga agacgttcga gttcggaaca tggggcggcg agctccgcga
     5161 cgaattccag ccccgcgcgg gcgacatcgt agcccaggag cattggtgtt caagtggttt
     5221 cgcgaacacg gatctggatc tgctgctcaa gaagcacggg atccacgagc tcatcgccgt
     5281 cggactcatc gcgcacacct gcgtcgaggc aactgttcgc tacggcgctg agctcggcta
     5341 cgaggtcacg gtggtgaagg acgcgacggc gagctactcc gaggatcaca tgcacgccgc
     5401 gctggaagtg aacatcccga actacgcaac tgccatcgtc accgcagacg agatcgccga
     5461 cgcacttcgc gctccagaac catgaccata ggtgaaggtc gggtggcatg ctcatggcgc
     5521 gcgtccgaag tcactgatcg cccccggaat tccacggcct caagcgtgga atccacgcgc
     5581 gcacgtttcg tcgatacgcc tcgtattcgt ttccgtaacg gcgggacaag gttggttcct
     5641 cataccagcg gacgaacgac gccgtcacaa cccacaccac tgcggcgtaa acaagcaagc
     5701 cgacgctgcc aaatagcaac gcctgaccca agattgccac cagcaggccg acgtacatcg
     5761 ggttgcggac gtatctattg aatccggtaa ccacgagacg ttcggttggc gccaccggca
     5821 tcggcgtgcc gcccgccttg gcgaactcca cgaacgcgag cgccaacggt atgagcccgg
     5881 caatgatcaa cggcgcgcct accgctacct gccatagcga gcccgagact ttccaacccg
     5941 tgatcaacca ggggatgaga ccgacaaccg tacccggggc caccacgaag aacgccgcac
     6001 tgccaaccgc ggccgtcgac tgtcgcatcg ttttcttcct tacgccgcct tgacaggttg
     6061 gtcgaccacg tcgaggctga ggaactccgg tgccttgata actctgcgga gcatgaaccg
     6121 gatgaacgcc ggcatatcgg tcgcattctc gtcgcggtaa acccgtgggg cggcgacccg
     6181 atagcgccgc ctgcgcgtct ccagttcaca gccgcccgcg gcgacgacat tgcgaaccca
     6241 gtcggcgtcg gaaccgtacg tcagcgcaat cacgaacccg ccccggcgac ggaaggccca
     6301 cagcggggtc cggaaggttc ggcccgatcg gcggccacgg tgtacgacga ccgcccatcc
     6361 cggcgcccac ggagcgatga acgcggtaag ccgattcagc ccaaccttgt tggcacgcgc
     6421 aacccatcgt ggagccggca tagtaaaact cctcacctgt ggaatttctc tgctccccca
     6481 cgctaacccc tgccgaacca aaagtcaacg cctgtagaat tcatgccact atgccacctc
     6541 ttggacgacg gccaggtaag acggacaccc gcaatgtctt gctgcagaat gctcgtcgga
     6601 tgtttgcgga gggtggctac aaccacacgt cggtccgcga catcgccgct gcggccggcg
     6661 tcgacccggc tatgatccgc cactatttcg gcagcaaggc acaactgttt cgagccacga
     6721 tgggatggcc tttcgatcct gggcagctcg ccgcgcagat tacgtcgggc gatcgcagcg
     6781 aaatcggagc acggctgacg caggtgttct tcggggcgtg ggagcagccg gactcgcgcg
     6841 ccccgctact ggcgattctg cggggcgccg caacccacga agaatcggca accctggtcc
     6901 ggcagttcat ccagactcag ctctatccac agatcgccgc cgcgcttgag gatccgaaag
     6961 ccgaacttgg ggtcgacctg gcgatggccc agctcctggg gatcgcctat ctccgccata
     7021 ttttgtgcgt cgaaccgatc gcttcgacac ccatcgacga actcgtggct cgggtcgcac
     7081 cagtgatctc cgcgcatctt gatttgagta ccggagccac gttgaacgaa cagtgaatcc
     7141 ttcgtcaata tcggcgggtg ccgcctaccg tcagcgacgt cacccacgcg ttgcactgtc
     7201 cgaccgatca tccccgcggg aacgcgccac gatgatcggc gcactcgacc ggctcgttga
     7261 tggaatgccc gtcggtcaac tcgctgcaga gcacgccaac gtcttgccgg ccaacaggcg
     7321 acccgcgatc cagatgtgag cgccaacatc gtcgggcggc cgatccacgg ttcggcgtaa
     7381 ttggtgaacc gttctgacga tcgtcgcgag cacgtctgca cgggtttcat ggcgcccgtt
     7441 gtcaaacagc tcgatcggct cttgacccga tacgttagcg aatactaacg tatcggtgtg
     7501 tgctcgacat gctcgaagtc gcagcagagc cgtctcggcg taggctgctg caactcctgg
     7561 cttcgggcga gcgcaccgtc acccagctcg cgtcgcactt cccggtcacc cgctcggcga
     7621 tatcgcagca tctcgcgatc ctcgccgagg tcggcttggt caccgcccgc aagcagggac
     7681 gggaacggta ttaccggctc gacgagggcg ggatggtgcg gctccgtgcg ttgttcgagt
     7741 cgttctggag cgatgagctc gaccgtctcg tctccgacgc cacccgaatt catcgcgaaa
     7801 cggaggctta gccatgccct ttgagaaggc agtcgtcgtg ccgctggatc cggccgagac
     7861 attcgcattg atcaccgggc cggacaggct tcggcgctgg atgaccgtcg ccgcccgcgt
     7921 agacctacgg gccggcggtg agtatcgctg gatcgtgacg cccgggcaca ccgcctccgg
     7981 ctcgatcgtc gatgtggatc cgggaaagcg ggtggtgttt tcgtgggggt gggaggacgg
     8041 ggatccggcg ccgggcgggt cgacggtgac ggtcacgctg acgccggtca ccggcggcac
     8101 cgaggttttg ctggtgcacg acggcctcaa cgacgagcag gccgcccgtc acgccgaagg
     8161 ctggaaccat tacctcgatc gcctggtggc cgcgggccaa cgcggcgacg ccggccccga
     8221 cgaatgggcg gcggcacctg agccgctcga cgaattgtcc tgtgccgagg ccacgttggc
     8281 ggttctgcaa catgtgctgt gcggtattga cgcgtccggc tcgtccaagc agaccccgtg
     8341 cagcgagtac gacgttgcag aactgggcga tcacctgatg aactcgatcg cgatcatcgg
     8401 cggtgcggcg gatgcgcaga tgccggctcg cgataccgcc gcgcctcttg aagccaaggt
     8461 cgccgacgcg gctcaggccg tactcgaggc ttggcgtcgg cgggggatgg acaacaccgt
     8521 ctcgctgaac tcgcggcaag tgcccgcggt cgtgccggtc ggcatcctgt cacttgaatt
     8581 cttggtgcac gcttgggatt tcgctatggc gaccggtcgt caggtggtag tttccgaacc
     8641 ggtgtcagag tttgtgctgg ccatcgccgg caaggtgatc acacccgagg cgcgcggcta
     8701 cgcgggcttc gctgaccctg ttgcgaccgc ggccgatgcc ggggttctcg atcggctcat
     8761 cgctttcacc ggccgtcaac aggccgtcgc tcacccaacc gcccactaga cgaaaggcca
     8821 atcatgccga agcgaaccga atacgcccag ggcacgccga actgggtcga cctgcagacc
     8881 accgatcagg acgcggccaa gcagttctac gcgtcgttgc tgggctggag ctacgatgac
     8941 cagccgatcc cgcagggcgg ggtgtactcc atggcaacgg tcaacggcga aaccgtggcg
     9001 gccatcgccc cgatgccgcc gggcgcgccg gagggcatgc cgccggtgtg gaacacctac
     9061 atcgcggtcg acgacgtcga cgcggcggtc ggcaaggtcg agcccgcggg cgggcaggta
     9121 ctgatgccag gtttcgatat cggcgatgcg ggcaggatgg cgttcatcgc tgatccgacc
     9181 ggggccgccg tcgcgctgtg gcaggccaac cagcacatcg gcgcgacgct ggtcaacgaa
     9241 accggcgcgg tcatctggaa cgaattgatc acggacaagc cggaatcggc gctggccttc
     9301 tatgaggcgg tggtcgggct ggagcactca atgatggaaa tggcgccggg tcagaactac
     9361 acgctcctca aggcgggggg atcggatgtc ggtggctgca tggaaccccc catgcccggg
     9421 gtgcccaacc actggcaggt gtacttcgcg gttgacgaca ccgacgccac ggcggctaag
     9481 gcctccgcgg agggcggcca gattgcggtg gagccgttcg acatcccgtc ggtgggacgg
     9541 tccgctgtgc tgaccgatcc gcagggcgca atgttcagcg tgctgcaacc gacacctcag
     9601 caatagcggc aggccgacgg cgtggcgagc accgcgcgct tcggtcaggg aatttccgtc
     9661 gagtacagct acgcatccca caacggctgc agcgactcgt cggagtagtt ggcggccagg
     9721 cgtgtgaagt cgtcggtgac gacggtgccg tgccggtagc cgctcgtccg atcctgcagc
     9781 agcgcaaaga atttcgcgcc gcttgaacat gtcgcgcggg ccgggatcgg ccagcagcag
     9841 gtcctggatt ctgcgatcgt tcggctacgc cccggacttc gttgtgcggg ggaagagcaa
     9901 tgccctgact gcccgctctg ccagcatctc cggatcttct cggatgccga cgtgttggaa
     9961 atcgctaccg atagtcaaag acgcgattcc gtggagcagc gaccaggtcg gtccggcgat
    10021 gtcacgcggg tcgccggccg gcagcaggtt cgcctcttgg catcgaactg tgacatcgag
    10081 taggtcgccg aaggcacgcg ccccggcggc gagcagctcg ggtttgtcac tcttgtccag
    10141 ctcggcgccg aacatcacct ggtagtagtc ggggtgggtg agcgcccagc gcacatatgc
    10201 ccgtcccatg tcgagcactt gccgtgtcag atcggtgttt tcggcggcgg cgttcagggc
    10261 ctcgtggagc tggacgaaac cttgcgtcgc ggcggcggcc aacagctgcg ctttgtccga
    10321 gaagtggcgg tatggcgcag cggcgctcac gcccgcacgc cgggcgacct ctctgagggt
    10381 gaagcctttc ggccccttct ccttgaccag ccccagcgcc gcctccgtga gtgcccgctt
    10441 caggtcgccg tggtgatagg tgtcgcgctg ctcacttgag atctcgggaa tcttctctcg
    10501 acgcgccatg ttgacgatgt taacatgatc ccatgttaac gccgtcaaca tctgcggaag
    10561 ggtttcaaaa tgtcgaagaa acgagaggtc agcgcgggtt gggccgtcgt caccggcgcg
    10621 agctcgggcc tgggcgcatt gttcgccgag aagctcgccc agcggggttt gccgcttgtg
    10681 ctctccgggc gagacgaagc gcgtctggcg gaggtgcggc agcgagttca gcgactcgct
    10741 cctggcgtcg acgtcgaact cgtggtcggc gatctcggca tccacgcggg cgtcgacgcc
    10801 ctggttgcgg tactcgacgg acgggtgatc gacgtcctgg tcaacaacgc ggggttcggc
    10861 acctacggcc ggctgccgga gatcgagccc gaccgggacc acgagctgat tgctgtcaac
    10921 gtcgacgcgt tggtgcgcct cacccatgca gttctgcccg gaatgctggc acgcgggcac
    10981 ggccgaattc tgaacgtggc gtcgaccatc gcgtttcagc ccgcgccgta tcaggccacg
    11041 tacggggcct cgaaggcgtt cgtgctgtcg ttcagtcagg ccctgtgggc cgagacgaga
    11101 ggctcgggcg tcacagtgac cgcgctgtgc cctgggccca cgcgcaccgg ctttgtcgac
    11161 gcgctggatg cggacgtctc gggcactgcg atataccgac ggctcgcttc accggaaacg
    11221 gttgtggccg caggacttcg agcgctcgat cgcggacgac cggtggtggt tccgggtctg
    11281 cggaaccggg tgatggccac cggcgcaagt ctgagccctg ggtgggtcgg ggcgctgatc
    11341 ggtggcagga tgcttcgtcc cgctcgcaac tctcgagccc acgttgcgtc gcaatgatca
    11401 ccggcagcgg tgtggaggca gccgacgacg cggcgcagat ggtcgccgcg gtgcgggatg
    11461 atcccgccgg ccggatccgt cttgccgccg acgcctacgc gtttcaccgc gggggtcggc
    11521 ggtatcggcc ctaccggcgg gcagtcgtcg cgttcatgcg gtggcagcag gcacggagtg
    11581 tcctcaattc gcttgacgac gacgtccccg gaagcccgtg gtggcgcgcg gtcaacgagg
    11641 acctcctccg cgacaccctc gaggcgaggc tgctcgtaga gcgcggcggg ggagccccgt
    11701 cgcggcccac cgtcgcgcgc tgggtcagct tcttcgacgc tccgtcggcg cagtcgtggt
    11761 acggggcgca caacgcgagc gtcgtcgccg ggtacctcgc gcactcctca ctcgctgcgc
    11821 tggaggtgcc cgccgagcga ttcttcatga acgtcgtgct cgtgcgcgtg ttgtacgcgc
    11881 acgcgctggt gcttgacggc gacctcgcgc tcggtcggtt gtcattcctc gcgcggctga
    11941 tcggtcaccc tcggtcgcgg ggtccgcagg cgttgcttgc aatgaaagac gttctgcccg
    12001 aacagtatcc gatccaggag gcgagggtag aggaactcat cgagagcgag aacagactcg
    12061 gccggatggt cgactacggt gtgatcggcg cacgtgtcga cgctctatac gcctcctccg
    12121 cgcgcgcgtt gggtgagccg ctcctgctcg acttgatcat cgacggagcg cccgcctacg
    12181 catggccggc cgatcaacgt cacgtgtgga agcctccgtc acagcggttg ttcacatcgt
    12241 tgatcgaatt cctgacccgc ccccgcaaca gccgcgtgca gctcgctggt atggcagtcg
    12301 gcattgccgg atgagcctgt gcgtcggctg ggaatgaatc cacgtgaacc ggcattgatc
    12361 cgcatcagcg agcaatgcac cggcgagagg acgacgacat gaccacatca aagcccttgg
    12421 cgggacgacg agcgctggtg acgggagggt cgcgcggcat cggcgcggag atcgtgcggc
    12481 gcctggccga cgacggcgcc gcggtcgcgt tcacctatgg cgcgtcggcc gccgacgccg
    12541 acaagctggc caccgaggtg gcggcacgtg gcggtaccgc cattgcgatc caggccgaca
    12601 gcgccgatcc cgagcaggtc gcgaacgcgg tcgacgcgac ggtatcgcgg ctgggcgggt
    12661 tagacattct ggtcaacaac gccggcgtcg cctacatcgg cgggatcgag tcgttcccgt
    12721 tggagcagtt cgaccggttg gtggcgatca acgtccgcgg cgtgttcgcc gcgatccagc
    12781 gcgcggtgcc gcacctgggc gacaacggcc gcatcatcac catcggcagc atcaacgccg
    12841 atcgggtgcc ggtgcctggg ctatcggtgt acgcgatgac gaaggccgcc gtcgccgggt
    12901 tgacccgcgg actggctcgt gagctcgggc cgcgcggcat cacggtcaac aacatccagc
    12961 ccggaccgat cgccactgac atgaatcccg aagagggcga attcgccgat gccgcaaggc
    13021 aagtcatcgc gctgggccgc tatggccgtc cgcgcgacgt cgccagcgtg gtcagctatc
    13081 tcgccggacc ggaatctgcg ttcatcaccg gcgtcaactg ggacgtcgac ggcggattcg
    13141 ccgcgtagcc cggctgagtg tcacagccgt ggcaggccga cagacactgc gcggttcaac
    13201 gcccaggctc ggcatcatcc acgatcagat tgatccgagt tagcaccggg cgagatccag
    13261 tgcgcgttcg gattcgcaga caacgaccat tcgctaggct cctcgatgtc agggtcacat
    13321 cgcgaatcag gagcccacta tgaacgccga tgcgaaggga agtggggaag acgcccgtcg
    13381 gctacgggag ttcaccgcaa tttctcggac gacgacctgg agcgtctcgt ccgagcagca
    13441 cagcgcacct cgacgtccgc gccatggccg ctgattcatg agcagactcc gtcggacgcc
    13501 tgctacatcc tgctcagcgg ggaggcaggg gtgtacgtcg gccgggaccg cgttg
//
LOCUS       DASWBF010000264         6024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_948242,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000264 DASWBF010000000
VERSION     DASWBF010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948242"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VF874_09690"
     CDS             complement(<1..523)
                     /locus_tag="VF874_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9176335.1"
                     /translation="MATPTPTRRTQEQRSAETRARLLDATIECLVRYGNTGTTTPRVA
                     EMAGVTRGAQVHHFGSKNDLVIAALQHLAAKRTAAAVGNVADLATTEDPIGEVLDLLW
                     DLHQGPMFVAAVELWVAGRTDPALGREVAKFESIVATNLMTAVAEFVPDEIHKPMLDF
                     LYTAMDALRGILIA"
     gene            636..1508
                     /locus_tag="VF874_09695"
     CDS             636..1508
                     /locus_tag="VF874_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9176336.1"
                     /translation="MREVKFVELHGDRVAYRDEGNGEALLLIHGMAGSSETWRAVLPR
                     LAKKYRVVAPDLLGHGQSAKPRGDYSLGAFAVWLRDFLDELGITHATIVGHSLGGGVA
                     MQFVYQHPDYCRRLVLISSGGLGPDVGAILRLLSAPGAEFVLPIVAPRPVLNVGNKLR
                     SWLSSAGIHSPRGAEIWSSYTSLADRQTRQSFLKTLRSVVDHRGQAVSALNRLKLRAD
                     LPMMAIWGDSDGIIPVDHAYAAQKARQDSRLEVLPGIGHFPQVEAPFEVVDLIEDFVS
                     TADRATVASEQPQP"
     gene            complement(1505..1669)
                     /locus_tag="VF874_09700"
     CDS             complement(1505..1669)
                     /locus_tag="VF874_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176337.1"
                     /translation="MRESAQPNRDQELEKIVDDLDENVMGEREAKDVPGKPSEREQEK
                     QEGSAEEPTA"
     gene            1785..2795
                     /locus_tag="VF874_09705"
     CDS             1785..2795
                     /locus_tag="VF874_09705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9176338.1"
                     /translation="MKVDFTLAGQPGDWDMAVSAEQRGYDCAWLPEIGHDPFPLLAIA
                     ASKTDRIQLGTAIAVTFARNPMSMALVANDLQLYSQGRFLLGVGSQVKAHITKRFSMP
                     WSAPAERMREFILAMRAIWHSWAIGEELHFVGEHYRHTLMTPFFNPGPNPFGNPPVIL
                     AGVGPKMTRVAGEVADGFFLHGFTTERYLREVTLPALREGRAAAGKDDLDGFEICGLP
                     FIVTGINEDEVRAADAAARAQIAFYGSTPAYRPVLDLHGWGHLGEELNQMSKRGQWSE
                     MGSRITDEMLHEFAIVAPPDQVAERLLAMYSDVFTRTGFYAPYRVADGFWDPIKATLQ
                     AA"
     gene            complement(2807..3907)
                     /locus_tag="VF874_09710"
     CDS             complement(2807..3907)
                     /locus_tag="VF874_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX9176339.1"
                     /translation="MNESTAAWRELLDTLGSLDRTFLAGDRAVTDDRHIADGYRMLAT
                     TLGVAFDTYLFAEPSRPIFVEVNTPFRRDRRWGGDNTDAYYFMCPVDPERRYRISGNR
                     GDSVYFSVTAYNEPSPGAWSDRIVAIVRDDDLDIDKDGNFSFEIGPTPDAAVLMTRDY
                     QADPLTGRPVSWRIEALDEPDPIRHGDAETAAALRTSAAWLRTMFAIVPLTVGVRVDD
                     AHTFGHEISQAANQFADPYQVPDANFGWSARDACYAYGSFVLEEDEALVITHRPPSCR
                     FWNLVVWNQFMAGPCVTDARTSVNGYSATTNADGSVTVVVSRGMTTHPNSVTTLDYPR
                     GNLAFRWFLADEVPARPEVRLVKVSDAPTSVD"
     gene            complement(3904..4479)
                     /locus_tag="VF874_09715"
     CDS             complement(3904..4479)
                     /locus_tag="VF874_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9176340.1"
                     /translation="MTTDLGRPRNPRIDGAVLDATVELLGQTGYAALSVDAIARRAGT
                     SKPAIYRRWPSKAHLVHEAVFPMNRETELPDTGTLAGDVREMVRRAMAVLTAPAARAA
                     IPGLVGEMAADLTLHAKLLKRFGDILSRGLTERLNDAAARGEVRPGVTAADLSEALAG
                     ITLMALLTRGAELDDAWVERTATLIMRGISA"
     gene            4508..5752
                     /locus_tag="VF874_09720"
     CDS             4508..5752
                     /locus_tag="VF874_09720"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9176341.1"
                     /translation="MTHAAALVQLDDLTKPRFSPEAEQIRDLMASMAADCPLDADALH
                     ARAVADTGLDDFGADDYRERLDVYLAALHEISGMHGAGIVNFYSQTLQLLKNRLLLTD
                     LLKRHPEIHDIELAPPVVIAGLPRSGTTHLHNLLAAAPTFRTLPYWESVEPFPLPAEV
                     GVEPDPRRVRMDAAVGFMDVVMPHFRLMHEMTTDHVHEEIQLLANDFSTMLFESLGHV
                     PRWRDYYLSHDQTSHYEHLATQLKALQFLRGGRRWLLKSPQHLEQLPVLDRVFPGVIV
                     VVTHRDPVPIVLSMIAMITYSARMHRSPVPVQEIAAYWVDRLELMLGALVRDRDVIGP
                     ERSVDVRFDDFMADELGIANQVYALAGEPMSDETRCAIADYLAGHRRGRLGQVATSCE
                     MFGLDEHDLHGRFAPYVERFLS"
BASE COUNT          978 a         1979 c         1976 g         1091 t
ORIGIN      
        1 tcgcgatcag gattccgcgt agtgcatcca tcgcggtgta gaggaagtcc agcatcggct
       61 tgtggatctc gtcgggaaca aactcggcga ccgccgtcat caagttggtc gccacgatcg
      121 actcgaactt ggcgacttcg cggccgagcg ccggatcggt gcggccggcg acccacaatt
      181 caactgcggc aacgaacatc gggccttggt gaaggtccca cagcaggtcg agcacctcgc
      241 cgatggggtc ttcggtggtc gccagatcag cgacattgcc gacggctgcc gccgtgcgtt
      301 tggcagcgag atgctgaagc gcggcgatca ccagatcgtt cttggagccg aagtggtgca
      361 cctgtgcacc ccgcgtcacg ccggccatct cagcgacccg cggcgtcgtc gtccctgtgt
      421 tgccgtagcg gaccaggcac tcgatcgtcg cgtccagcaa ccgtgcgcgg gtctcagcgc
      481 tgcgctgttc ctgcgtgcgg cgcgtcggcg tcggagtcgc cacacaagat acataccagt
      541 atgtatgtaa gtttgccagg gctctccctt gatttcgcca gactctccgg tccctgcgat
      601 gatgggcaga actggacgga aaagggggac ccgtcatgcg cgaggtcaaa ttcgtcgaac
      661 tgcacggcga ccgggtcgcg taccgcgatg agggcaacgg cgaggcactg ctgttgatcc
      721 acggcatggc cggcagctcg gaaacctggc gcgctgtgct gccgcgactg gcgaagaaat
      781 accgagtggt cgcgccggat cttctcggcc atggccagtc ggcgaagccc cgaggcgact
      841 actcgctcgg cgcgttcgcg gtatggctgc gcgacttcct cgacgagctg ggcatcaccc
      901 acgccacgat cgtcggccac tcgctcggcg gcggcgttgc gatgcagttc gtgtatcaac
      961 accccgacta ctgccgccga ctggtcctca tctccagcgg cggactgggc cccgacgtcg
     1021 gcgcgatcct gcggctattg tcggcccccg gagccgagtt cgtgctaccg atcgtcgcgc
     1081 ctcggcccgt gctcaacgtc ggcaacaagc tgcggtcgtg gctgagttcg gccggaatcc
     1141 attcgccgcg gggtgccgaa atctggagct cgtacacgtc gctggcggac aggcagacgc
     1201 ggcagtcgtt cctgaagacg ctgcgctcgg tggtcgacca tcgcgggcag gcggtcagcg
     1261 cgctcaaccg cttgaagctg cgggccgacc tacccatgat ggcgatctgg ggcgacagcg
     1321 atggcatcat ccctgtcgac cacgcctatg ccgcccagaa ggcgcgtcag gacagccggc
     1381 tggaggtgct gccgggcatc ggccacttcc cacaggtgga agccccgttc gaggtggtag
     1441 atctgatcga ggacttcgtc agcaccgccg accgggcgac tgtggcgagc gagcagcctc
     1501 agccctaggc ggtcggctct tcggccgaac cctcctgctt ctcctgctct cgctcactgg
     1561 gcttgcccgg cacgtccttc gcctcacgct cgcccatgac gttctcgtca aggtcgtcga
     1621 cgattttctc cagctcttgg tcccggttgg gttgcgcgga ttccctcatc tcgctctcat
     1681 cgctagtacg tcgggccgac agccgacccg agggtgcgtg gtcaagtctc cacggtgtga
     1741 cctgtaaccg tccacttacc cgcgccgcct atggttgaac cgtcatgaaa gtcgacttca
     1801 ctctcgccgg ccagcctggt gactgggata tggcggtgtc agccgagcaa cgcggctacg
     1861 actgcgcgtg gcttccggag atcggccatg atcctttccc gttgcttgcg atcgcggcga
     1921 gtaagacgga cagaatccag cttggcactg cgattgcggt gacgttcgcg cgcaatccca
     1981 tgtcaatggc gttggtggcc aatgacttac agctgtactc tcagggccgc ttcctgcttg
     2041 gggtgggctc acaggtgaaa gcccatatca ccaagcgctt ctcaatgcct tggtccgctc
     2101 cggccgagcg catgcgcgag ttcatccttg cgatgcgggc gatttggcac agctgggcca
     2161 ttggcgagga actgcatttc gtcggcgagc actaccgcca caccctgatg accccgttct
     2221 tcaaccccgg gcccaacccg ttcggcaacc cgccggtgat cctcgccgga gtggggccga
     2281 agatgacgcg ggtggccggt gaggtggccg acgggttctt cctgcacggg ttcaccaccg
     2341 agcgctatct gcgggaggtg acgttgccgg cgctgcgaga aggacgggcc gcggccggca
     2401 aggacgacct cgacgggttc gagatctgcg ggctgccgtt catcgtcacc ggtatcaacg
     2461 aagacgaggt ccgggccgcc gacgccgcgg cgcgcgcgca gatcgcgttc tacggctcga
     2521 cgcccgcata ccgcccggtg ctggacttgc acggctgggg gcatctgggc gaggagctca
     2581 accagatgtc caagcgcggt caatggagcg agatgggcag tcggataacc gacgagatgc
     2641 tgcacgagtt cgcgatcgtc gccccgcccg atcaggtcgc cgaacggtta ctcgcgatgt
     2701 attcagatgt gttcacccgc accgggttct acgcgccgta ccgggtggcg gacggcttct
     2761 gggacccgat caaggccacg ctgcaagctg cctagccgcc aggacgtcag tcaacgctcg
     2821 tcggcgcgtc ggacaccttc accagacgca cctccggccg ggcgggaacc tcgtcggcga
     2881 ggaaccaccg gaaggcgagg tttccccgcg gatagtcaag cgtggtaaca gaattcggat
     2941 gcgtcgtcat tccgcgggag acgaccaccg tcaccgagcc gtccgcgttg gtcgtcgcgc
     3001 tgtagccgtt gaccgacgtt cgcgcatcgg tgacacacgg gccggccatg aattggttcc
     3061 acaccaccag gttccagaac cggcacgacg gcggccggtg ggtgatcacc agcgcctcgt
     3121 cctcctcgag cacgaaactg ccgtaggcgt agcaggcgtc gcgcgccgac cacccaaagt
     3181 tggcgtccgg cacctggtag gggtcggcga attgatttgc ggcttgggag atctcgtgcc
     3241 caaatgtgtg cgcgtcgtcc acccgcacac cgacggtcag cggcacgatc gcgaacatgg
     3301 tccgcagcca ggcggcgctg gtcctcaagg ctgcggcggt ttcggcatcg ccatgccgga
     3361 tcggatcggg ttcgtcgagc gcctcgatcc gccagctgac gggccggccg gtcagcgggt
     3421 cggcctggta gtcacgggtc atcagcacgg cggcgtcggg cgtcggcccg atctcgaacg
     3481 agaagttgcc gtccttgtcg atgtcgagat cgtcgtcgcg cacgatcgcc acgatccggt
     3541 ctgaccacgc gccgggcgac ggttcgttgt aggcggtcac cgagaaatag acgctgtcgc
     3601 ctcggttgcc gctgatgcga tatcggcgct cgggatcgac tgggcacatg aagtagtagg
     3661 catcggtgtt gtcgcctccc caccgtcggt cgcgtcggaa cggcgtgttc acttcgacga
     3721 agatcggccg gctcggctcg gcgaagaggt aggtgtcgaa ggccacgccc aacgtggtgg
     3781 ccagcatgcg gtagccatcg gcaatgtgcc ggtcgtcggt gacggcgcgg tcacccgcga
     3841 ggaacgtgcg gtcgagactg cccagggtgt cgagcagttc gcgccatgcc gcagtggatt
     3901 cgttcatgcg ctgattcctc tcatgatcag ggtggccgtg cgctccaccc acgcgtcgtc
     3961 gagttccgcg ccgcgggtga gtagcgccat caacgtgatg ccggcgagtg cttcggacag
     4021 atcggccgcg gtcacgccgg gccgcacctc gccccgcgcc gcggcgtcgt tcagtcgctc
     4081 ggtcagaccg cgcgacagga tgtcgccgaa ccgcttcagc agcttggcgt gcaaggtgag
     4141 gtccgcggcc atctcgccga cgagtccggg tattgccgcg cgcgccgccg gcgcggtcag
     4201 cacggccatc gcgcggcgca ccatctcgcg cacgtcgccg gccagcgtcc cggtgtcagg
     4261 aagctcggtc tcgcggttca tcgggaacac cgcctcgtgc acgaggtgcg ccttgctcgg
     4321 ccaacgccgg tagatcgccg gcttgctggt gccggcgcga cgagcgatcg cgtcgaccga
     4381 aagcgcggcg tatccggttt ggccgaggag ttcgaccgtc gcatccaaca ccgcaccgtc
     4441 gatgcgtgga tttcgggggc ggcctaggtc tgtcgtcatt acgtaacgca cagtaacata
     4501 agtcgacatg acccacgctg cagctctcgt ccagctcgac gacctcacca agccccgctt
     4561 cagtcccgag gcggagcaga tccgcgacct catggcttcg atggcggccg actgtccgct
     4621 cgacgccgac gccctgcacg ccagagccgt tgcggacact ggcctcgacg atttcggcgc
     4681 cgacgactac cgggagcgcc tcgacgtcta cctcgcggca ctgcacgaga tcagcggcat
     4741 gcacggggcc gggatcgtga acttctacag ccaaacgctg cagctgctga agaaccgctt
     4801 gctgctcact gacctactca agcggcaccc cgagatccac gacatcgagc tcgcgccgcc
     4861 ggtcgtcatc gcgggtctgc cccgcagcgg caccacgcat ctgcacaacc tgctggcggc
     4921 cgcgccgacg ttccgcacgt tgccgtattg ggaaagcgtc gagccgtttc cgctgccggc
     4981 cgaggttggt gtcgagccgg atccgcgccg ggtgcggatg gatgctgcgg tcgggttcat
     5041 ggacgtcgtg atgccgcatt tccggctcat gcacgagatg accaccgacc atgtccacga
     5101 ggagattcag ctgctcgcga acgacttctc gacgatgctg ttcgaatccc ttgggcacgt
     5161 tccccgttgg cgcgactatt acctatccca cgaccagaca agccattacg agcatctcgc
     5221 cacccaactc aaagcgctgc agttcctgcg cggcggccgg cgctggctgc tgaagtcacc
     5281 gcagcacctc gagcagctgc cagtgctgga ccgcgtgttc cccggcgtga tcgtcgtggt
     5341 cacgcatcgc gatccggtgc cgatcgtgct gtcgatgatc gcgatgatca cctactcggc
     5401 gcgcatgcac cgcagcccgg tgcccgtgca ggagatcgct gcctactggg tcgaccgcct
     5461 cgagctcatg cttggcgcgc tcgtgcggga tcgggatgtc atcgggccgg agcgctccgt
     5521 cgacgtccgc ttcgacgact tcatggccga cgagctgggg atcgccaacc aggtgtacgc
     5581 tttggccggt gagccgatga gcgacgaaac acgttgcgcg atagccgatt acctcgccgg
     5641 gcaccggcga gggcggctcg gccaggtggc gacgtcatgc gagatgttcg ggctcgacga
     5701 gcacgacctg cacggtcgtt tcgcaccgta cgtcgagcgc tttttgtcct agttcgactg
     5761 cgtcgtgggt ccggcggacg acccgggcgg cgtatcggtg ttcgacggtt tgttcgcacc
     5821 gttcgtaatg ctgtcagcca gatggttgag accggacgtg aagtcgccaa tcgcctgccg
     5881 caaagggctt ttggtaccgg tggacgacga agtggtcggc ttattgccgc ctaggactgg
     5941 cttgaacccg ttggaaatgt caaacgggtt cgtgctcggc ttggacacct gcggcagtga
     6001 ccccgccggc gaagtgctca ccaa
//
LOCUS       DASWBF010000265         6226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1272155,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000265 DASWBF010000000
VERSION     DASWBF010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272155"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      1..111
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            147..2978
                     /gene="gcvP"
                     /locus_tag="VF874_09725"
     CDS             147..2978
                     /gene="gcvP"
                     /locus_tag="VF874_09725"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX9176342.1"
                     /translation="MSTAFAERHIGPSDDDVATMLNVIGVDSLDELAAKAVPTGILDA
                     LQGDVAPGLDVLPPAASEEEAFTELRRLADANTVPVSMIGQGYFDTLTPAVLRRNIIE
                     NPAWYTAYTPYQPEISQGRLEALLNFQTMIADLTGLEIANASMLDEGTAAAEAMTLMH
                     RAARGASNRLAVDTDLYRQTAAVLDTRAEPLGIEIVTADLRDGLPDGEFFGVIAQVPG
                     ASGAVADWTALVDAAHERGALVALGADLLALTLIIPPGEVGADVAFGSAQRFGVPMGF
                     GGPHAGYLAVHSKHARQLPGRLVGVSVDADGSPAFRLSLQTREQHIRRDKATSNICTA
                     QVLLAVLAAMYASYHGADGLKSIARRVHGHARALAAGLSAADVEVVHRSFFDTVLARV
                     PGRAAEIHEAAKSRGINIWMVDDDHVSVSCDEATTDTSIGHVLDAFKATPVDDVFDGP
                     EIATRTSEFLTHPAFTRHRTETEMMRYLRALSDKDIALDRSMIPLGSCTMKLNAAAEM
                     EPITWPEFARQHPFAPETDTPGLRRLISDLETWLADMTGYDAVSLQPNAGSQGEYAGL
                     LAIHAYHAGRGEPHRDVCLIPSSAHGTNAASAALAGMRVVVVACRQNGDVDLDDLRAK
                     VAEHAKDLAALMITYPSTHGVYEHDIADICAAVHDAGGQVYVDGANLNALVGLARPGR
                     FGGDVSHLNLHKTFCIPHGGGGPGVGPVAVREHLRRHLPGHPLADELSDEPPVSSAPY
                     GSASILPITWAYIRMMGASGLRAASLTAIASANYIARRLDEYYPVLYTGENGMVAHEC
                     ILDLRGITKATGVTVDDVAKRLADFGFHAPTMSFPVPGTLMVEPTESESLAEVDAFCD
                     AMIAIRAEIDKVGSGEWSADDNPLRGAPHTAACLLVDTWEHPYTREQAAYPLGRGFRT
                     KVWPPVRRIDGAYGDRNLVCSCPPVEAFA"
     gene            2994..3863
                     /locus_tag="VF874_09730"
     CDS             2994..3863
                     /locus_tag="VF874_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9176343.1"
                     /translation="MLPASVAEWHDGGRWISTSVGNVFTRSAEGAGPTILLLHGYPSS
                     SYDFRNVVPHLAGRAWVTMDFLGFGLSDKPRPHRYSLLEQADLVQTVVAETTSGPMVV
                     LAHDMGTSVATELLARDIHGRLPFELQRAVLTNGSVILKRASLRPSQKLLRSRLGPAF
                     ARLINRQGFTRGFGKLFSQDHPLSAEEAAAQWALLSHNGGHRIAHLLISYLDERERYA
                     QRWHGAVRDWPKPLGFLWALKDPVATTNVLDGLRELRPSAPVIELPGLGHYPQVEDPD
                     AFTAGALRLLTES"
     gene            complement(3867..4595)
                     /locus_tag="VF874_09735"
     CDS             complement(3867..4595)
                     /locus_tag="VF874_09735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9176344.1"
                     /translation="MSSIDRRTLMKAVAFGVLAAMTRPAAAHARPTAPTGRIPPPTGV
                     LTRLPGDGNLLALTVDDGASTDVVSAFAAFCRDSGTRLTFFVNGANASWSINAPQLRP
                     MVDSGQVQLGNHTWSHPYVTRVSPAAATDQIQRNKDFLQNTYGVDGTPYFRPPYGVHN
                     AATDKIAADLGYPTITLWSATIGDSRVINEQQLVNYANQAFVGQQIVLAHANLPPVTH
                     TYPQLLDIIASRGLQTVTLNDVFG"
     gene            4767..>6226
                     /locus_tag="VF874_09740"
     CDS             4767..>6226
                     /locus_tag="VF874_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9176345.1"
                     /translation="MTAEASAPPLIQPRRPFPARLGPKGNLIYRVITTTDHKLIGIMY
                     MVACMVFFFIGGLMALLIRAELAVPGLQFLSNEQYNQLFTMHGTAMLLFYATPIVFGF
                     ANIVLPLQIGAPDVAFPRLNALSFWLFVFGALIALSGFITPGGAADFGWTAYSPLTDA
                     IHSPGAGGDLWILGLGVGGLGTILGGVNMITTIICMRAPGMTMFRMPIFTWNILVTSI
                     LVLLVFPLLTAALFGLAADRHLGAHIYDPANGGAILWQHLFWFFGHPEVYIIALPFFG
                     IVTEVFPVFSRKPLFGYTTIVYATFSIAALSMAVWAHHMFATGAVLLPFFSFMTFLIA
                     VPTGIKFFNWIGTMWKGQLTFETPMLFAIGFLVTFLLGGLSGVLLASPPIDFHVTDSY
                     FVVAHFHYVLFGTIVFATFAGVYFWFPKMTGRMLDERMGRLHFWLTLIGFHTTFLVQH
                     WVGNEGMPRRYADYLPTDGFETLNIVSTIGSFILGAS"
BASE COUNT          970 a         2049 c         2088 g         1119 t
ORIGIN      
        1 cacgatgcct ctggaaagcg gtgacgaccc cgttcgtcgc ccgccgatgg ggaaaggcgc
       61 aagccgaatc tctcaggcgc ccggtaccgg gtcaaagaca gagggagagg acaagccgtc
      121 ctctgccgcc gtcaggagtc ccgctagtgt ccaccgcatt cgccgagcgc cacatcggtc
      181 ccagcgacga cgacgtcgcc acgatgctga acgtcatcgg tgtcgattcg ctcgacgaac
      241 tggcggcgaa ggccgtcccg acgggaatcc tggacgcgct gcagggcgac gtcgcacccg
      301 gtctggacgt gctgccacct gcggccagcg aggaggaggc gttcaccgaa ctgcggcgcc
      361 tcgccgacgc caacaccgtc ccggtgtcga tgatcggcca aggctacttc gacacgctga
      421 cgcctgcggt gttgcggcgc aacatcattg agaacccggc ctggtacacc gcgtacacgc
      481 cgtaccagcc ggagatcagc cagggcaggc tcgaggcgct gctgaacttc cagacgatga
      541 tcgccgacct gaccggtctg gagatcgcca atgcgtcgat gctcgacgag ggcaccgccg
      601 ccgcggaggc gatgacgctg atgcaccgtg ccgcccgcgg cgcgtcgaac cggctcgccg
      661 tcgacaccga cctgtaccgc cagacggcgg ccgtgctgga cacgcgcgcg gaaccgctgg
      721 gcatcgagat cgtcaccgca gacttgcgtg acggcttgcc cgacggcgag ttcttcggcg
      781 tgatcgcgca agttcccggc gccagcggcg cagtggccga ctggactgcc ctcgtcgacg
      841 cggcgcacga gcgcggcgcg ctggtggcgc tgggtgccga tttgctggcg ctgactttga
      901 tcatcccgcc cggcgaggtc ggcgccgacg tcgcgttcgg cagtgcccaa cgcttcggcg
      961 tgccaatggg tttcggcggc ccacatgccg gatacctcgc ggtgcacagc aagcacgccc
     1021 gccagttgcc gggccggctg gtcggggtgt ccgtagacgc ggatggctcg ccggcgttca
     1081 gattgtcgct gcagacccgc gagcagcaca tccggcgcga caaggcgacc agcaacatct
     1141 gcaccgccca ggttctgctg gcggtgctgg ccgcgatgta cgccagctac cacggcgccg
     1201 acgggctgaa gtcgatcgcg cgccgggtgc acggccacgc ccgcgcgctg gccgccggtc
     1261 tgtcggcggc ggatgtcgag gtggtgcacc ggtcgttctt cgacaccgtg ctcgcccggg
     1321 tgcccggccg tgccgcggag atccacgagg cggccaagtc gcgcgggatc aacatctgga
     1381 tggtcgacga cgaccacgtg tccgtgtcgt gcgacgaggc cacaaccgat accagcatcg
     1441 gccacgtgct ggacgcattc aaggccactc ccgtcgacga cgtgttcgac ggacctgaga
     1501 ttgctacccg cacaagcgaa ttcctgacac atcccgcgtt cacgcgccac cgcaccgaaa
     1561 ccgagatgat gcgctacctg cgggcgctgt ccgacaagga catcgcgttg gaccgcagca
     1621 tgattccgct cgggtcgtgc acgatgaaac tcaacgcggc cgccgagatg gagcccatca
     1681 cgtggccgga gttcgcccgt cagcacccgt tcgcgcccga aacggacacg ccgggactgc
     1741 gccggctgat ctccgatctc gagacttggc tggcggacat gaccgggtac gacgcggtgt
     1801 cgctgcagcc caatgccggc tcgcaggggg agtacgccgg gctgctcgcg atccacgcgt
     1861 accacgccgg ccgcggcgag ccgcaccgcg acgtgtgcct gatcccgtcc agcgcgcacg
     1921 gcaccaacgc cgcgtcggcc gcgctggccg gcatgcgggt ggtggtggtc gcgtgccgcc
     1981 agaacggcga cgttgacctc gatgatctgc gcgccaaagt cgctgagcac gcgaaagacc
     2041 tagccgcgct gatgatcacg tatccgtcca cccatggggt gtacgagcac gacatcgccg
     2101 acatctgcgc cgcggtgcac gacgcgggcg ggcaggtgta tgtcgacggc gcgaacctca
     2161 acgcgctcgt cggactggcc cgtccgggcc ggttcggcgg cgacgtcagc cacctcaacc
     2221 tgcacaagac gttctgcatc ccgcacggtg gcggcgggcc gggcgtcggg ccggtcgcgg
     2281 tgcgcgagca tctgcggcgc cacctgccgg gccaccccct cgccgacgaa ctcagcgacg
     2341 agcctccggt gtcgtcggcc ccgtatggct cggcgtcgat cctgcccatc acctgggcct
     2401 acatccgcat gatgggcgcc tcaggcctgc gtgcggcgtc gttgaccgcg atcgcatcgg
     2461 ccaactacat cgcccgccgt ctcgacgagt actacccggt gctctacacc ggcgagaacg
     2521 gcatggtggc ccacgagtgc atcctggatt tgcgcggcat caccaaggcc actggcgtca
     2581 cggtcgacga tgtcgcaaag cggctggcgg acttcggctt tcacgcgccg acgatgagtt
     2641 ttccggtgcc cggcacattg atggtcgagc caaccgaaag cgagagcttg gccgaggtcg
     2701 acgcattctg cgacgcgatg atcgcgatcc gcgccgagat cgacaaggtc ggttctggcg
     2761 agtggtccgc cgatgacaac ccgctgcgcg gcgccccgca caccgccgcc tgcctgctcg
     2821 tggacacgtg ggagcacccc tacacccgcg aacaggccgc ctacccgctc ggtcggggct
     2881 ttcgaacgaa ggtgtggccg ccggtacgtc gcatcgacgg cgcgtacggc gaccggaatt
     2941 tggtgtgctc gtgtcctccg gtggaggcgt tcgcctgaag tactgtcacc gacatgcttc
     3001 ccgcgagcgt ggccgagtgg cacgacggcg ggcgctggat ttcgacgagc gtgggcaacg
     3061 tgttcacccg ctccgctgaa ggcgccggcc cgacgatcct gctgctgcac ggctatccgt
     3121 ccagttcgta tgacttccgg aacgtcgtgc cgcacctcgc cggccgcgcg tgggtgacga
     3181 tggactttct cggcttcggc ctgtcggaca agccgaggcc acaccgctac agcctgctgg
     3241 agcaggccga cctggtgcag accgtggtcg ccgagaccac gtctggaccc atggtggtgc
     3301 tcgcacacga catggggacg tcggtggcca ccgagctgct ggcacgagac atccacggcc
     3361 gcttgccttt tgagctgcag cgcgcggtac tcaccaacgg cagcgtgata ttgaaacggg
     3421 ccagcctgcg gccgagtcag aagctgctgc gcagccggct ggggccggcg ttcgcgcggc
     3481 tgatcaaccg gcaggggttc acccgtggct tcggcaagct gttctcacaa gaccatccgc
     3541 tgtcggcgga ggaggctgcg gcgcagtggg cgctgttgtc gcacaacggc ggtcaccgca
     3601 tcgcccatct gctgatctcc tacctcgacg agcgcgagcg gtacgcgcag cgctggcacg
     3661 gcgcggtgcg cgactggccg aagccgcttg ggtttctgtg ggcattgaag gacccggtgg
     3721 caacgacgaa cgtgctcgac gggctgcgcg agctacggcc gtcggcgccg gtcatcgagc
     3781 tgccggggct gggccattac ccgcaggtgg aggaccctga cgcgttcacc gcaggcgcgt
     3841 tgaggctgct caccgagtcg tgactgtcag ccgaacacgt cgttgagcgt gactgtctgc
     3901 aggccgcggc tcgcaatgat gtcgagcagt tgcgggtagg tgtgggtgac gggcggcagg
     3961 ttggcgtggg cgagcacgat ctgttgaccg acaaacgcct ggttggcgta gttgaccagt
     4021 tgctgttcgt tgatcactcg tgagtcaccg atcgtcgcgc tccacaacgt gatcgtggga
     4081 tagccgagat cggccgcgat cttgtccgtc gcggcgttgt gcactccgta cggcggacga
     4141 aaatacgggg tgccgtccac gccgtaggtg ttctgcagaa aatccttgtt gcgctggatc
     4201 tgatcggtgg ctgcggccgg gctgacccgg gtgacatagg gatgcgacca ggtgtgattg
     4261 cccagctgaa cctggccgga gtcgaccatc ggccgcagct gcggcgcgtt gatcgaccat
     4321 gacgcgttcg cgccgttgac gaaaaacgtc aatcgtgtgc cgctgtcacg acagaacgcc
     4381 gcgaacgctg acaccacatc ggtgctcgcg ccgtcgtcga cagtaagcgc caacaggttt
     4441 ccgtcgccgg gcagacgggt gagcacgccg gtcggcggcg gtatccggcc ggtcggcgcg
     4501 gtgggccgtg catgcgcggc cgccggccgg gtcatcgccg ccaacacccc gaacgcaacg
     4561 gctttcatca atgtgcgtcg atcgatgctc gacacatgct caccgtaacg atccggcgcg
     4621 gcggcgacgt gtccttgagc tgccagttcg ctgtgtggtt tctggcactc gggtggtggc
     4681 cgcgccgacc cttgcgaaat gtttcgccaa cggctcgcgc cgcatagcat cgggggcgac
     4741 caacgtcgga tgtccaggga agagccatga ccgcagaagc gtccgcaccg ccactgattc
     4801 agccaaggcg acccttcccc gcgcgtctgg gtcctaaggg caatctgatc taccgggtga
     4861 tcactacgac cgatcacaag ttgatcggga tcatgtacat ggtcgcctgc atggtgttct
     4921 tcttcatcgg cgggctgatg gcgctgctga tccgcgccga gttggcggtg cccgggctgc
     4981 agttcttgtc caacgagcag tacaaccagt tgttcaccat gcacggcacg gcgatgctgc
     5041 tgttttatgc gactccgatc gtgttcggct tcgccaatat cgtgttgccg ctgcagatcg
     5101 gggcgcccga tgtggcgttc ccgcggctga atgcgttgtc gttttggctg tttgtgttcg
     5161 gggcgctgat cgcgctgtcc gggttcatca ccccgggggg tgcggcggat ttcggctgga
     5221 cggcctacag cccgttgacc gatgccatcc acagtccggg tgcgggcggt gacctgtgga
     5281 ttttggggtt gggcgtgggt ggtctgggca ccatcctggg cggggtcaac atgatcacga
     5341 cgatcatctg catgcgcgcc ccgggtatga cgatgtttcg gatgccgatc ttcacctgga
     5401 acattctggt gaccagcatt ctggtgctgc tggtgttccc gctgctgacc gcggcgttgt
     5461 tcgggctggc cgccgaccgt cacctgggcg cgcacatcta tgacccggcc aacggcggtg
     5521 cgattttgtg gcagcacctg ttctggttct tcggccaccc cgaggtgtac atcatcgcgc
     5581 tgccgttctt cggcatcgtg accgaggtgt tcccggtgtt ttcccgcaag ccgctgttcg
     5641 gctacaccac gatcgtgtac gccacgttca gcatcgccgc gctgtcgatg gcggtgtggg
     5701 cgcaccacat gttcgccacc ggcgcggtgc tgttgccgtt cttctcgttc atgacgtttt
     5761 tgatcgcggt gcccaccggg atcaagttct tcaactggat cggcacgatg tggaagggcc
     5821 agttgacgtt cgagacgccg atgttgttcg cgatcggctt cttggtgacg ttcctgctcg
     5881 gtgggctgtc gggggtgctg ctggccagcc cgccgatcga cttccacgtc accgacagct
     5941 actttgtggt ggcgcacttc cactacgtgc tgttcggcac gatcgtgttc gccaccttcg
     6001 ccggggtgta tttctggttc ccgaagatga ccggccggat gctcgacgag cggatgggcc
     6061 ggctgcattt ctggctgacg ctgatcggct ttcacaccac gttcttggtg cagcactggg
     6121 tgggcaacga gggcatgccc cgccgctacg ccgactacct gcccaccgac ggcttcgaga
     6181 cgctcaacat cgtgtccacc atcggctcat tcatcctggg cgcctc
//
LOCUS       DASWBF010000266         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_948936,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000266 DASWBF010000000
VERSION     DASWBF010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948936"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..936
                     /locus_tag="VF874_09745"
                     /pseudo
     CDS             344..936
                     /locus_tag="VF874_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
     gene            complement(950..1549)
                     /locus_tag="VF874_09750"
     CDS             complement(950..1549)
                     /locus_tag="VF874_09750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9176346.1"
                     /translation="MVSRTSTRPQVDSARGWPFYEVDSARLWDVPETPVPVGIAVSGE
                     KSVHMFAPIGDHLIAVEPDEGLVRAWQHARQATGLPGHGRVIGQIPVSWDPDGDTAVK
                     RAHDQFRWFAGGWHVNADLPTTAGFAAATQFVRPEDVAESIPCGPDLDAIVDAVREYW
                     EAGFTDIALVQIGDDGQDRFLAEAAEPLLAKLREAAPQH"
BASE COUNT          254 a          529 c          516 g          265 t
ORIGIN      
        1 gacgagaagc cgcgaatcgt gttgcggcgc gtacacaccg acgtcgttga cgatcgtctc
       61 acaggggtcg gggaaggtaa tcgtcaccgg atgctcgcat ccgacgagtg aagaaacacg
      121 atcatgctgg cctttcgacg acggtctcag gatcgatcgg gtaattaatg ccctcgaaaa
      181 cctgacctca aacccacgtc agacgagtgt cacacttcag cagtgtttgt cgggtcggcc
      241 gttcgggcac ccggcaggcg cgaaagcgtc cgacatcgtt gcgttgcccc ttcagttggg
      301 atcggccgtt cgccgccggc gggtgtttca tcccgtgggg gtgatggcga aaggttcttt
      361 cgagcgactc gcaccgcccg gcgagggact gccggtggag tcgtcggagg tggtgggtcg
      421 cgtgtcgaaa ggcatcggcc tgcccggtgg cctgcccgac atcatcgggc tcgcgatccg
      481 cattccgccg caaccgttcg cggcgacgcc gtgggacatc ttgatggcgt cggccggctc
      541 cggcgttctc acccggttcg cgttgcggcc ggtcacgtcg tggcacgcgc cgatgtcgag
      601 cctgatgccg ctgcgctacg acgggaagta ctggtgggtc cgtgcgaagc tgacgtccga
      661 tctggacgcg gaggggctct cgctcgacga cgtcgccgag cagatcgatc gcgacggtgt
      721 ggagttcgaa ctcggccagg ccgccgggat taacgacttc gaaccgctcg cccgggtgca
      781 ttcaatgaag tcctgcccgc tggcccgtcg aacgaggtcg cgttcgaccc gacgatccac
      841 actgcgcccg gccccaagct cgcgccggaa tggctgaccg acatccgccg gcgcgcgtac
      901 gagcgcagcc gtgagggtcg ccccgactcc gcctgagcca caagctgttt cagtgctggg
      961 gtgcggcctc gcgcagcttg gccagcagcg gttcggccgc ctctgcgagg aagcggtcct
     1021 ggccgtcgtc accgatctgc accaaggcga tgtcggtgaa cccggcctcc cagtactcgc
     1081 ggacggcgtc gacgatcgcg tcgagatccg gcccgcacgg aatgctctcc gccacgtcct
     1141 ccgggcgcac gaactgcgtc gccgcagcga agcccgcggt ggtcggcagg tcggcgttga
     1201 cgtgccagcc gccggcgaac cagcggaact gatcgtgtgc ccgcttcacg gcggtgtccc
     1261 cgtcgggatc ccaggacacc gggatctgac cgatcacccg gccatgaccc ggcagcccgg
     1321 tcgcctgccg tgcgtgctgc cacgcacgga ccaggccctc gtccggctca accgcgatca
     1381 ggtggtcacc gatcggcgcg aacatgtgca ccgacttctc ccccgacacg gcgatcccga
     1441 ccggcacggg agtctccgga acgtcccata gccgcgcgga atcgacctcg taaaagggcc
     1501 acccgcgggc actgtcaact tgaggtcgcg tggacgttct gctaaccaca tactaacttg
     1561 actt
//
LOCUS       DASWBF010000267        18852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_285272,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000267 DASWBF010000000
VERSION     DASWBF010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18852
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285272"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VF874_09755"
     CDS             complement(<1..1563)
                     /locus_tag="VF874_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9176347.1"
                     /translation="MTPLRTTLSQLRLRELLVEVQDRVEQIVEGRDRLDGLVEAMLVV
                     TSGLELDDTLRTIVHTAIDLVDAKYGALGVRGHDHELVEFVYEGIDDETRAKIGRLPE
                     GRGVLGVLIDDPKPIRLEHILEHPDSVGFPPNHPPMHTFLGVPVCIRDEVYGNLYLAE
                     KANGQPFSEDDEVLVRALAAAAGIAVDNARLYAQARARQSWIEATRDIATELLSGSDP
                     TRVFRLIADEALQLCAATATFVAVPVDAELPTNEVAQLLVVETAGDTEIAASQTIPVA
                     ETEIGDVFTQRTPRRLESIELGMNGVDTNGPALVLPLRTTDTVAGVLVALRHKGEQHF
                     DGEQLDMLAGFADQAALAWQLATTQRHLRELDILSDRDRIARDLHDHVIQRLFAVGLS
                     LQGTIPRARSAEVQQRLSNCVDDLQAVIQEIRTAIFDLHGASSGVTRLRQRLDEAVSQ
                     FSTPEVRTMVQFVGPLSVVSPTLADHAEAVVREAVSNAVRHADATTLRVSITVEDDLH
                     IEVVDNGKGIPAD"
     gene            complement(1633..2280)
                     /locus_tag="VF874_09760"
     CDS             complement(1633..2280)
                     /locus_tag="VF874_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9176348.1"
                     /translation="MIRVFLVDDHEVVRRGLFDLLDADPELEVVGEAASVGEALAKIP
                     SVPPDVAVLDVRLPDGSGIELCRELLSDMPELRCLMLTSFTSDEAMLDAILAGASGYV
                     VKDIKGMELAKAIKDVGAGKSLLDNRAAAALMAKLRGAAERSDPLSGLTAQERTLLGL
                     LGEGLTNKQIAARMFLAEKTVKNYVSRLLAKLGMERRTQAAVFATKLGPASRPTE"
     gene            complement(2356..3222)
                     /locus_tag="VF874_09765"
     CDS             complement(2356..3222)
                     /locus_tag="VF874_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9176349.1"
                     /translation="MREKPAPPSIVVGVDGSKAAIHAALWAVDEALSRDIPLRLLHAV
                     DPGEAAEGAEHAARKLAVAETAIREVVMAIEATDKPVKIEVEIDQDRPVSALIRASRP
                     AAMICVGAVGFHHFQPGRVGSTAAAVATSAHCPVAIIHAHDGGHVRRDPGWIVVEADN
                     SPHTGVLLEAAMDEARLRNAPLRIITCWQSRVSDIRGSRSVADTNRRVQAELDRRLAR
                     WTHHYPDLDVQSVAVHGSVIDYLAENVHSVQLVVVGARDHRTVQQLVGPSGYGALRHT
                     NCSVLVVDHQHL"
     gene            3331..4443
                     /locus_tag="VF874_09770"
     CDS             3331..4443
                     /locus_tag="VF874_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9176350.1"
                     /translation="MEHAAQSAMLEGLQPLIEVLTGRGYRVVGPTLRDNAIVLAELES
                     ADDLPRGWGVDTAPGHYRVRRREDEAAFGHSSGPQSWKQFLHPPRQRLWSGGRDGVAD
                     GEPEEPPRYAFLGVRGCDLAAIATLDRVLGYSQHPDSTYVGRRRQIFVVAVNCTEPGG
                     LCFCASMGTGPAAGPGYDLALTERLDGDAPTYLVDVGTPDGADVLAAIPHRSADAGEI
                     EGARKEVDAATSRMGREMPSVDLRELLMRNRESPQWEEVASRCLTCGNCTMVCPTCFC
                     TSTEDVSDLTGDNAERWQHWASCFEFDFTHVHGGSVRQSGPSRYRHWITHKLGTWHDQ
                     FGMSGCVGCGRCIAWCPTGIDITEEMTTMARRDGDD"
     gene            4436..5326
                     /locus_tag="VF874_09775"
     CDS             4436..5326
                     /locus_tag="VF874_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX9176351.1"
                     /translation="MTDTAVWDTLSPTSAMTPVPYRVCSRVVENSDSVTLLLEPVAAA
                     LPTPQPGQFMMLYAFGVGEIAISVSGDPTVTGRRYGTRARFDGTIMNTIRAVGAVSRA
                     LCASESGAVIGVRGPFGTRWGLDEAAGRDLVIVAGGVGLAPLRPVVAGALARRDRYGK
                     VVLIAGARSRKDFLYVDELAGWQRDRSIEVHTTVDVPVAGWRGEVGMVTGPLRRLTLT
                     AERTTAFLCGPEPMMRFSAEALLAKGLSVNDIRVSLERNMQCGIAWCGHCQLGPLLLC
                     RDGPVVDYEVAYPLLVVKEL"
     gene            5335..6117
                     /locus_tag="VF874_09780"
     CDS             5335..6117
                     /locus_tag="VF874_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX9176352.1"
                     /translation="MSHSGEEQKVKPTLAVWKFASCDGCQLTLLDCEDELLTIAEQIE
                     IAMFAEASSAMVGGPYDVSLVEGSVTTPHDQERIREIREQSKVLVAIGACATAGGIQA
                     LRNFADVAEFAKVVYASPEYIDTLATSTPASAHVTVDYELRGCPIDRGQLLDTLAALL
                     IGRKPKLPASTVCTECKRSGVTCIAVAEGIPCLGPVTHAGCGALCPRHHRGCYGCFGP
                     AATPNTLALFPVLRRDGLSDDDVDRVYSTFNVAQFAAQRGEK"
     gene            6126..7418
                     /locus_tag="VF874_09785"
     CDS             6126..7418
                     /locus_tag="VF874_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX9176353.1"
                     /translation="MTTVTRTVRVGTLTRVEGEGALNVTLRDHALESVQLNIYEPPRF
                     FEAFLRGRKYTEPPDITARICGICPVAYQISACNAIEQACGVTVDPALVELRRLLYCG
                     EWIHSHALHIYLLNGPDFLGYPDAISLARDHREAVERGLALKKAGNRLQELIGGRAIH
                     PINVRLGGFYSVPTRGQLDAMAEELRRALDHALDTVRWVSEFEFPELEFEHELLSLSQ
                     PGRYPIENGTIVRSGGPGFSAAEFCDHVEESHVPHSTSLHATLDGRPHLTGPLARYSL
                     NASLLSPIARQAAAEAGLTPECRNPFRAIVARSVEVVYAIEEALRIIDAYERPARPFV
                     DVPPRHGVGHGVSEAPRGLLYHRYEVSDDGLIAAATIVPPTAQNQGAIEADLARLISA
                     NLDVADEQLTGLCENLIRTYDPCISCATHFLTLTVRRM"
     gene            7415..7906
                     /locus_tag="VF874_09790"
     CDS             7415..7906
                     /locus_tag="VF874_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX9176354.1"
                     /translation="MTADIVVMGIGNSFRRDDGVGPAVAAAVNARAIPDVSVLIDAGD
                     PAVVLDAWAGVRLAVLIDAAVVTPSVPGRIHRCTKDQLAGSPAVSSHGVDLATLLALG
                     EALGRIPDDVAVFAVEADETGYGVGLTPDVAAAVPLVVDAVLAEINAETEGRQRSWAP
                     TGS"
     gene            7885..8988
                     /locus_tag="VF874_09795"
     CDS             7885..8988
                     /locus_tag="VF874_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX9176355.1"
                     /translation="MGTHWLVMDDAKDAARAPYRARLAAAGRHLPATRLTTDELMSTT
                     RHHTHIDLERLTGVHERRVSVGDEDSYSLATTAARNSLATAKLDGSSLDVVINCSITK
                     FHGGITNWLEPTMSSAVARAIGAPNAMTFDISNACAGMLTGVTVLNNWIRQGVVERGL
                     VVSGEYISPLGRNAARHIRTIMSKELACLTLGDAGAALLLERAGEGPGAISLAGFTTI
                     ADHSRLCLAWPKGRDPGARMFTDARAIQRVAISDTPLLLHEVLDMAGIAIHDIDHVIT
                     HQTSAIAIRKGMAELSELFGDRPRHDAVITVDRYGNTASTTHTVALVEELEAGRILPG
                     ETIALIALASGLEIGVILVTVDEDLVNRYGHDR"
     gene            8972..9844
                     /locus_tag="VF874_09800"
     CDS             8972..9844
                     /locus_tag="VF874_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX9176356.1"
                     /translation="MGTIVDRVEIAHAGWRGRHSALHLAVVAAKSCLHAAQREADDVD
                     LLVNAGTYRDKNLGEPALAALIQEDIGANPEDPHEGAHGTFSFDIANGTCGILTGLQI
                     VDGFLRSHTISCALVIASDADPGHGMSEHFPFSPVGAALLCRWTDGDYGLGRVSWVNE
                     LDGGENFRATVGSDDARNVLRFHESPAFDERLATAAAHAVRDCLQSEGLTLSDVGAVV
                     AAPARQGYRAALARQLDVSSERVIVGDDERTNTAALAAAMQRAMKDGPIDVLLVAAGA
                     GITAGAALYREPPV"
     gene            9939..10775
                     /locus_tag="VF874_09805"
                     /pseudo
     CDS             9939..10775
                     /locus_tag="VF874_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738096.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10867..11838)
                     /locus_tag="VF874_09810"
     CDS             complement(10867..11838)
                     /locus_tag="VF874_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEX9176357.1"
                     /translation="MGSPGEHIQYGTTIITLTMNPALDVTTSADRVGPTDKIRCRGVR
                     YDAGGGGINVARIARVLGGSVSAVFPAGGHTGDLVTQLVAEADVPYRRVDITESTRES
                     FTIDEQCSGQQYRFVLPGPRLSFVEQARCLDELRDAARSAEFVVASGSLPPGVPADFY
                     QRVADMCRELGVLLVLDSSGGGLRHITSGVFLLKPSVRELRERMGRDLVSEADQLAAA
                     HELIDAGITQTVVVSLGSKGALLATPHGSQRFSPVPVKVFSGVGAGDAMVAAITVGLT
                     RGWSMGKSVRFGIATGSAMLMTPGTAPCHRSDAERIFETSPEPEDIV"
     gene            complement(11879..13918)
                     /locus_tag="VF874_09815"
     CDS             complement(11879..13918)
                     /locus_tag="VF874_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX9176358.1"
                     /translation="MTRTTRSAPSAPRRVFRDRREAGRILAQLLTAYRGKDDVVVLGL
                     PRGGIPVAWEVAAALGAPLDAFVVRKLGAPGHEEFAVGALASDGRVVINDDVVRGLGI
                     TPQQLRDVAEREGRELIRRETAYRGGRHPVDVTGKTVILVDDGLATGASMFAAVQALR
                     EAHPAEIVIAVPAAPESTVREFAGLVDDVVCASMPTPFLAVGESFWNFSQTSDEEVCE
                     LLATPTTGVARPAVKAAETPAQVIAHVAVDAPGGVPPTEALEELIGDARIVLIGESSH
                     GTHEFYEARAAITKWLIEQKGFCAVTAEADWPDAYRVNRYVRGLGDDTTPEEALRGFE
                     RFPAWMWRNTVVRDFVGWLRRYNEGRGAERQCGFYGLDLYSLHRSMQEVIAYLDNVDP
                     VAAERARKRYACFDHASADDGQAYGFAAAFGAGLSCEKQAIEQLVEMQRNALAYARRD
                     GLLADDELFYAQQNAVTVRDAEVYYRSMFSGRVTSWNLRDTHMAQTLDALLTHLDRHP
                     DGKPARIVVWAHNSHVGDARATEMGADGQLTLGQLAREKFGGDTRLIGFTTYSGAVTA
                     ASDWGGPAERKVVRTALNGSVEELFHETANPSFLVSPMLSSAAAEPLSVVRLGRAIGV
                     IYLPATERQSHYFHNRPADQFDAIIHIDRTSALEPLEVTSLWVEGETPETYPTGL"
     gene            complement(13932..14210)
                     /locus_tag="VF874_09820"
     CDS             complement(13932..14210)
                     /locus_tag="VF874_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1876 domain-containing protein"
                     /protein_id="HEX9176359.1"
                     /translation="MSHSGDQVKSWTVQVEIDEHENKTRAKARLEWRGRDYVGIGVAR
                     RNPADYNVAEIGDELATARALSDLGHQLLVVTASDIQAVTHEPVTNLH"
     gene            complement(14217..14648)
                     /locus_tag="VF874_09825"
     CDS             complement(14217..14648)
                     /locus_tag="VF874_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9176360.1"
                     /translation="MSNLERRQQRSLFPELADLFAGFPSFANVRPVFDARLMRLEDEM
                     TKDGRYEVRAEIPGVDPAKDVDITVHDGRLTIKAERTEKSESGGRSEFSYGSFERTVT
                     LPQGADEDDIKATYDKGILTVSVGVSEPKKTEKKIEIESSK"
     gene            complement(14744..15394)
                     /locus_tag="VF874_09830"
                     /pseudo
     CDS             complement(14744..15394)
                     /locus_tag="VF874_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
     gene            15560..16444
                     /locus_tag="VF874_09835"
     CDS             15560..16444
                     /locus_tag="VF874_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9176361.1"
                     /translation="MTGPVTHQGILVGVDGSAPSNAAVRWAAQEAATRNLPLTLVHAR
                     KTPTAGTTEMAWPAVPLPERLTRWQEEEAQQVLADSVQVAEGELTSSRLNTKVIFSAP
                     IPTLIDLSKDAAMVVVGCRGQGALARTLLGSVSTALVHHAHCPVAVIHYAVPARSEWP
                     VLVGIDGSPASELATAIAFDEASWRGANLVALHAWNDADAYAVPSLEWSALEVAADKT
                     LAERLAGWEERYPDVAVRRVVVWEHPARHLIERSEVSQLVVVGSHGRGGFAGMLLGSV
                     STAVVHNAHTPVIVARQN"
     gene            complement(16441..17532)
                     /locus_tag="VF874_09840"
     CDS             complement(16441..17532)
                     /locus_tag="VF874_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176362.1"
                     /translation="MRSDRFHPLLTARGPFVSVYFDDSHDTADALAQLNARWRDLYKH
                     LEDAGADQAVISRLEDAVLHRRPAVGRRGRGIIATAEETFVNEQLTEPPPVTTLRISD
                     YPYLLPLVEFGSWRPTYIVAAVDHAGADVILHQGDVVRKHTVDGGGYPVHKSPAAEGE
                     FAHPQPRVEEAIRKNVRAVAENLTDLVRDTSPEVVFVSGEVRSRTDVVSALPARVAQL
                     VHQVNAGVHSRDIDDNAIGGLVDAEFDRRRRAETADVMERFQAERGRGSGLAAEGLGP
                     VCAALRAGDVDTLIVGELADATVVTGEDRAAVAPSADVLSEWGQAAVRVARADEALPF
                     AAIAVGASLVRVDSLDGVAALLRYASSEG"
     gene            complement(17535..18419)
                     /locus_tag="VF874_09845"
     CDS             complement(17535..18419)
                     /locus_tag="VF874_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9176363.1"
                     /translation="MAQSTSRTGILVGVDGSAESDAAIRWATTEAVMRRAPLTLVHAL
                     EPMVTAWPIGPVQASITEWQEDNAGQVLDQAVKTVQSCAAQSELPDVHTGVLRSAIVP
                     ALVEASEDKQMIVVGSQGTGALGRMLLGSVSAGLLHHARCPVAVIRAGRQPSPDAPIL
                     LGIDGSPASEAATALAFDEASRRGVGLNVLHAWSDVAVFPMLGMDWREYESEGHEVLG
                     ERLAGWHEQYPDVAVQRRLVCDQPARWLIRESEHAQLVVVGSRGRGGFRSMLLGSVSS
                     AVAQAAAVPVIVVRGEWV"
     gene            complement(18430..18819)
                     /locus_tag="VF874_09850"
     CDS             complement(18430..18819)
                     /locus_tag="VF874_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9176364.1"
                     /translation="MTWARDAVRIFNKHALNPVMMRMAGRKHWYAGVIRHTGRRTGKE
                     YATPVVADRVAGGFIVPLPYGTGVDWLRDVLAADRATIQAGGQTYQVVHPVVIDAEAA
                     ATQLYPRRRRAFARFGIDRYLKIELAD"
BASE COUNT         2994 a         6389 c         6346 g         3123 t
ORIGIN      
        1 gtcggcaggg atgcctttcc cgttgtcgac gacctcgatg tgcaagtcgt cctcgacggt
       61 gatgctgacc cgcagagtgg tcgcgtcagc atgccgaacc gcgttcgaaa ccgcttcgcg
      121 cacaacggct tctgcgtgat ccgccagcgt cgggctgacg acggacagcg ggcccacgaa
      181 ctgcaccatg gtgcgcactt cgggcgtgga gaactgcgag acggcttcgt ccagccgttg
      241 ccgcagcctc gtgacacccg acgaggcgcc gtgaaggtcg aagatcgcgg ttctgatctc
      301 ctggatgacc gcttgcaggt cgtcgacgca gttcgaaagc cgttgctgca cttcggctga
      361 ccgcgcacgg gggatcgtgc cctgcagcga caacccgacc gcgaacaggc gctggatcac
      421 gtggtcgtgc aggtctcgtg cgatccggtc gcggtcgctg aggatgtcca actcccgtag
      481 atgacgctgg gtggtggcca actgccatgc cagtgcagcc tggtcggcga agccggcgag
      541 catatcgagc tgttcgccgt cgaaatgctg ctcgcccttg tgtcgcagtg ccaccagcac
      601 accggcgacg gtatcggtgg tgcgcagagg cagcaccagg gccgggccgt tggtgtccac
      661 accgttcatt ccgagctcga tagactccag ccggcgcggc gtgcgttggg tgaagacgtc
      721 gccgatctcc gtctccgcca ccggaatcgt ttgcgacgca gcgatttcgg tgtcaccggc
      781 cgtctctacg accaagagtt gggcgacctc gttggtgggc aactccgcgt cgacggggac
      841 cgccacgaac gtcgcggtgg cggcgcagag ctgcagcgcc tcgtcggcga tgaggcggaa
      901 cacccgggtc gggtcgctgc cggacagcag ctcggtggcg atgtcgcggg tggcctcgat
      961 ccaggactga cgggctcgcg cctgcgcgta cagccgcgcg ttgtcgaccg cgatgcccgc
     1021 cgcggcggcc agcgcccgca ccagcacctc gtcgtcctcg ctgaacggct gaccgttcgc
     1081 cttctcggcg agatacaggt tcccgtatac ctcgtcacgg atgcacaccg gtacaccgag
     1141 gaacgtgtgc atcggcgggt ggttgggtgg aaacccgacc gagtcgggat gctcgaggat
     1201 gtgctccagc cggatcggct tggggtcgtc gatcagcacg ccgagcacac cgcggccctc
     1261 gggcagccgg ccgatcttgg ctctcgtctc gtcgtcgatg ccctcgtaga cgaactcgac
     1321 cagctcgtgg tcgtggcccc gcaccccgag cgcgccgtac ttcgcgtcga ccaggtcgat
     1381 cgcggtgtgc acgatcgtgc gcagcgtgtc gtcgagttcg agaccggacg tcaccaccaa
     1441 catcgcctcg acgagcccgt cgaggcggtc tctgccctcg acgatctgct cgacgcggtc
     1501 ctggacctcg accagcagct cgcgtagccg cagctgggac agcgtggtgc gcagtggagt
     1561 catccgtcgg ctggttcggt cgggcacgcc attatcgtgg catcgtcgac gcgaagttgg
     1621 cgagtagttc ggctattcgg tgggacggct ggccgggccg agtttggtgg caaagaccgc
     1681 cgcctgggtg cgacgctcca ttccgagttt ggccagcaac cgcgacacgt agttcttcac
     1741 cgtcttctcg gcgaggaaca tccgcgccgc gatctgcttg ttggtgagcc cctcaccgag
     1801 aagtccgagc agcgtgcgct cctgcgctgt cagcccggac agcggatctg accgctcggc
     1861 cgcgccgcgc agcttggcca tcagcgccgc cgccgcccgg ttgtcgagaa gcgacttgcc
     1921 cgccccgaca tccttgatcg ccttggccag ctccatgccc ttgatgtctt tgaccacgta
     1981 cccgctcgcg ccggcgagga tcgcgtcgag catcgcttcg tcggaggtga acgacgtgag
     2041 catcaggcag cgcagttcgg gcatgtcgga aagcagctcc cgacacagct cgattccgct
     2101 gccgtcgggc agccgcacat ccaggaccgc gacgtcgggc ggcaccgacg gaatcttggc
     2161 cagcgcttcg cccaccgagg cggcttcgcc caccacctcg agctcagggt cggcgtcgag
     2221 caaatcgaac agcccccgac ggacgacttc atggtcgtcg accagaaaca ccctaatcac
     2281 ggccagccct ttcagctctg gatgcggcgc cggtcacaat gcgcatgttc atcatggcct
     2341 actcggtcag tgaactcaca agtgttggtg gtcgacgacg agcactgagc agttggtatg
     2401 acgcagcgcg ccatagcctg acggcccgac gagctgctgc acggtccgat ggtcgcgggc
     2461 gccgacgacc accagctgca ccgagtgcac gttctcggcg aggtagtcga tgacgctgcc
     2521 gtgcaccgcc accgactgca cgtcgagatc cggatagtgg tgcgtccagc gcgccagccg
     2581 ccggtccagc tccgcctgca cccggcggtt cgtgtcggcc acgctccgcg aaccgcggat
     2641 gtcgctgacc cgggattgcc agcaggtgat gatccgcagc ggcgcgttac gcagccgcgc
     2701 ctcgtccatc gccgcctcca acagcacgcc ggtgtgcggt gagttgtcgg cttcgacgac
     2761 aatccagccc gggtcgcgtc ggacgtggcc gccgtcatgc gcgtggatga tcgcgaccgg
     2821 gcagtgtgcc gacgtggcga ccgcggcggc ggtcgagccg acccgtccgg gctggaagtg
     2881 gtgaaaccca accgcgccga cgcagatcat cgcggccgga cgcgacgcgc ggatcaacgc
     2941 gctgacgggg cggtcctggt cgatttcgac ttcgatcttg acgggcttgt cggttgcttc
     3001 gatcgccatc acgacttcgc gtatcgcggt ctcggcgacc gccagcttgc gcgcggcgtg
     3061 ttcggcaccc tctgcggcct ctccgggatc gactgcatgc agcagccgca gcgggatgtc
     3121 tcgactgagg gcctcgtcga cggcccacag tgcggcgtgg atggccgctt ttgagccgtc
     3181 gacaccgacg acgatcgagg gcggagcggg cttttcgcgc atcgactcca ccataagtcc
     3241 tcgttcctct gtcgccgtag ggacttttgg ccgtaaggtc aaagcgaaac ctttatccga
     3301 agcagtctta ctcgccatag gatcgggcaa atggaacatg ccgcgcagtc cgcgatgctg
     3361 gagggcctgc agccgctgat cgaggtcctg acgggccgcg gttaccgggt cgtgggaccc
     3421 acgctgcgcg acaacgcgat cgtgctggcc gagctcgaat ccgctgacga cttaccccgc
     3481 ggctgggggg tggacacggc gcccggccac taccgggtgc ggcgccgcga agacgaggcc
     3541 gcgttcggtc attcctccgg accgcagtcg tggaaacagt tcctgcaccc accgcggcag
     3601 cggctgtggt caggcggtcg agacggcgtt gccgacgggg agccagagga gccgccgcga
     3661 tatgcgttcc tcggcgtgcg cggatgcgat ttggccgcga tcgccaccct cgacagggtg
     3721 ctgggttact cccagcatcc ggacagcacc tatgtcggcc gtcggcgcca gatcttcgtg
     3781 gtcgcggtga actgcaccga acccggcggc ctgtgcttct gcgcctcgat gggcaccggc
     3841 ccggccgccg gtcccggcta cgacctggcg ctgacggagc ggctggacgg cgacgcgccg
     3901 acctaccttg tcgacgtcgg cacccctgac ggcgccgacg tgctcgcggc gataccgcac
     3961 cgttcggccg acgcgggcga aatcgagggc gccagaaagg aagtcgacgc cgcgacttct
     4021 cggatgggcc gcgagatgcc gtccgtcgac ttgcgtgagc tgctgatgcg caaccgcgag
     4081 tcgccgcagt gggaggaggt ggcgagccgg tgcctgacct gtggcaactg cacgatggtg
     4141 tgcccgacgt gtttctgcac gagcaccgag gacgtcagcg acctgaccgg tgacaacgcc
     4201 gaacgctggc agcactgggc gtcctgcttc gagttcgact tcacccacgt ccacggcggc
     4261 agcgtgcggc agtccggccc gtcgcgctac cggcattgga tcacgcacaa gctgggcacg
     4321 tggcacgacc agttcgggat gtccggatgc gtggggtgcg gacggtgtat cgcgtggtgc
     4381 cccaccggga ttgacatcac cgaggagatg acgacgatgg cgcggcggga cggcgatgac
     4441 tgacaccgcc gtttgggata cgttgtcgcc gacgtcagcg atgacgccgg tgccgtaccg
     4501 ggtttgctca cgtgtcgtcg agaacagcga ctcggtgacc ctgttgttgg agccggtggc
     4561 ggccgcattg ccgacgccgc agccggggca gttcatgatg ctgtacgcgt tcggcgtcgg
     4621 cgagatcgcg atctcggtca gcggcgaccc cacggtcacc ggacgccgtt atggaactcg
     4681 tgccagattt gacggcacga tcatgaacac gatccgcgct gtcggggcgg tgagccgcgc
     4741 gctgtgcgcc tccgagtcgg gtgcggtcat cggggttcgc ggaccgttcg gcacccggtg
     4801 gggcctcgac gaggcggcgg gccgcgacct cgtgattgtc gccggcggag tgggactggc
     4861 gccgttgcgc ccggtggtgg cgggtgcgct ggcccgtcgg gaccgctacg gcaaagtggt
     4921 tctgatcgcg ggcgcgaggt cgcgcaagga cttcctgtac gtcgacgaac tcgccgggtg
     4981 gcagcgcgac cggtcaatcg aggtgcacac caccgtcgac gtcccggtgg cgggctggcg
     5041 cggcgaggtg ggcatggtca ccgggccgct gcgccggctc acgttgacgg cggaacgcac
     5101 caccgctttt ctgtgcggcc ccgagccgat gatgcggttc agtgcggaag cgctgctggc
     5161 aaagggtctg agcgtcaacg acattcgtgt gtcgttggag cgcaacatgc agtgcgggat
     5221 cgcttggtgc ggccactgcc agctcgggcc actgctgctc tgccgagacg gtcccgtggt
     5281 ggactacgag gtcgcctatc cgctgcttgt ggtgaaggag ttgtgatggc ggagatgagc
     5341 cactcgggcg aagagcagaa ggtgaagccc accctggcgg tgtggaagtt cgcgtcgtgc
     5401 gacggctgcc agctgacttt gctcgactgc gaggacgagc tgctgacgat cgccgagcag
     5461 atcgagatcg cgatgttcgc cgaggcgtcg agcgcgatgg tcggcggacc gtacgacgtg
     5521 tcgttggtcg agggctccgt cactactccg cacgaccaag agcgcattcg cgaaatccgg
     5581 gagcagtcaa aggtgctcgt cgccatcggg gcgtgcgcca cagcgggcgg catccaggcg
     5641 ctgcgcaact tcgccgacgt cgccgaattc gccaaggtgg tgtacgccag tccggaatac
     5701 atcgacacgc tggccacgtc gacacccgcg tcggcgcacg tcaccgtcga ctacgaactg
     5761 cgcgggtgcc ccatcgaccg cgggcagctc ctcgacacac tggccgcact gctgatcggg
     5821 cgcaagccga aactcccggc gtcgacggtg tgcaccgagt gcaaacgcag cggagtgacg
     5881 tgtatcgctg tcgccgaggg cattccgtgc ctcggcccgg tcacccacgc cgggtgcggc
     5941 gcactgtgcc cgcgccacca ccgcggctgc tacgggtgct tcgggccggc cgcgacaccg
     6001 aacacgctgg cgttgtttcc ggtgttgcgc cgcgacggcc tgtccgacga cgacgtggac
     6061 cgggtgtatt cgacgttcaa cgtcgcgcag ttcgcagccc agcgaggtga aaagtgacca
     6121 tcgacatgac caccgtaacc cgcaccgtgc gagttggcac gctgacgcgg gtggaaggcg
     6181 aaggggccct taacgtcacg ctgcgcgacc acgcgttgga gagcgtgcag ctcaacatct
     6241 acgaaccgcc gcggttcttc gaggcgttcc tgcggggccg caagtacacc gagccgccgg
     6301 acatcaccgc gcgaatctgc ggcatctgcc cggtcgccta ccagatcagc gcctgcaacg
     6361 cgatcgagca ggcgtgcggc gtgacggtgg atccggcgct ggtagaactg cgccgccttc
     6421 tctactgcgg cgagtggatc cacagccacg ccctgcacat ctacctgttg aacgggccgg
     6481 acttcctcgg ctaccccgac gcgatcagcc tggcccgcga ccaccgcgag gcggtggagc
     6541 gcggcttggc gctgaagaag gcgggcaacc ggctgcagga gctgatcggt gggcgcgcga
     6601 ttcatccgat caacgtgcgg ctgggcgggt tctattcggt gccgacacgt ggccagttgg
     6661 acgcgatggc cgaggagttg cgccgggccc tcgatcacgc actggacacc gtgcgctggg
     6721 tgagcgaatt cgagttccct gagctggaat tcgagcacga gctgctgtcg ctcagccagc
     6781 cggggcgata tcccatcgag aacggcacga tcgtgcgcag cggcgggccg gggttcagcg
     6841 cggccgagtt ctgcgaccac gtcgaggagt cgcatgtgcc gcactccacc tccctgcacg
     6901 cgaccctcga cggccggccc cacctcaccg gcccgttggc gcgctattcg ctgaacgcgt
     6961 cgcttctctc gccgatcgcg cggcaggctg ccgccgaggc agggttgacg cccgaatgcc
     7021 gaaacccgtt ccgcgccatc gtggcccgtt cggtcgaggt ggtctatgcg atcgaggagg
     7081 cgttgcggat catcgacgcc tacgagcgcc cggcgcggcc gttcgtcgac gtcccgccgc
     7141 gtcacggcgt cggccacggg gtcagcgaag cgccgcgcgg cctgctctac caccgatacg
     7201 aggtcagcga cgacgggctc attgcggcgg ccaccatcgt cccgccgacg gcgcagaacc
     7261 agggggcgat cgaagccgac ttggcccgcc tcatctcagc gaacctcgac gtcgccgacg
     7321 agcagctgac gggcctgtgc gagaacctga ttcgcaccta cgacccctgc atctcttgcg
     7381 cgacccattt cctgaccttg acggtgcggc ggatgtgacc gcagacatcg tcgtgatggg
     7441 catcggaaac agcttccgac gcgacgacgg tgtcgggccc gctgtcgccg cggccgtcaa
     7501 cgcccgcgcg atccccgatg tttcggtcct gatcgatgcc ggcgaccccg ccgtcgtgct
     7561 ggacgcgtgg gcgggtgtta ggctggccgt gctcatcgac gccgcggtcg tcacgccgtc
     7621 ggtgccgggc cgtatccacc gctgcaccaa ggaccaactc gccggctcgc ccgccgtgag
     7681 ctcgcacggc gtggacctcg ccacgctgct cgcgctgggg gaggcgctcg gccggatccc
     7741 tgacgacgtg gccgtgttcg ccgtcgaggc ggacgagacc ggctacgggg tggggttgac
     7801 gcccgacgtg gccgccgccg taccgctcgt cgttgacgcg gtgctggccg agatcaacgc
     7861 ggaaacggaa gggaggcaac gctcatgggc acccactggc tcgtgatgga cgacgcgaag
     7921 gatgccgccc gtgcgccgta ccgcgcccgg ctggccgcgg cgggccggca cctgcccgcc
     7981 acccggctca ccaccgacga gctgatgtcg acaacacggc accacacaca catcgacctg
     8041 gagcggctga ccggcgtcca cgagcggcgc gtctcggtcg gcgacgagga ttcgtacagc
     8101 ctggccacca cggccgcgcg caatagcctg gccacagcga aactcgacgg ctcgtcgctt
     8161 gacgtggtga tcaactgcag catcacgaaa ttccacggtg gcatcaccaa ttggctggag
     8221 cccaccatga gcagcgccgt cgcgcgtgcg atcggcgcgc ccaacgcgat gaccttcgac
     8281 atttcgaatg cgtgcgctgg gatgctcacc ggcgtaaccg ttctgaacaa ctggatccgg
     8341 cagggggtcg tcgagcgcgg gctggtggtc agcggcgagt acatctcccc gctgggccgc
     8401 aacgccgccc gccatatccg cacgatcatg agcaaggaac tggcctgcct cacgctgggc
     8461 gacgcgggcg cggcgctgct gctggaacgc gccggggaag gccctggtgc gatcagcctg
     8521 gccggtttca ccacgatcgc cgatcacagc agactgtgcc tggcgtggcc gaagggccgc
     8581 gacccgggcg cccgaatgtt caccgatgcc agggcaattc agcgggtcgc gatctcggac
     8641 accccgctgc tgctgcatga agtgctcgat atggcgggca tcgcgatcca cgacatcgac
     8701 cacgtgatca cgcatcagac gtcggccata gcgatccgca agggcatggc cgagttgtcg
     8761 gagttgttcg gtgaccgtcc ccgccacgac gcggtgatca ccgtcgaccg ctacggcaac
     8821 acggcgtcga cgacgcacac cgtggcgctg gtcgaagagc tcgaggcggg ccgcatcctg
     8881 cccggagaga cgatcgcgct gatcgcgctg gcatcgggtc tggagatcgg cgtgattctt
     8941 gtcaccgtgg acgaggatct ggtgaaccgc tatgggcacg atcgttgacc gggtggagat
     9001 cgcccacgcc ggttggcgcg ggcgtcacag cgcgctgcac ttggcggtgg tcgctgcgaa
     9061 gtcctgcctt cacgcggcgc agcgggaggc cgacgacgtc gatctgttgg tgaacgccgg
     9121 cacctaccgc gacaagaatc tcggggaacc ggcgcttgcc gcgctcatcc aggaagacat
     9181 cggcgccaat cccgaagacc cgcatgaggg tgcgcacggc acgttctcct tcgacatcgc
     9241 caacggcacc tgcggaatcc tcaccgggtt gcagatcgtg gacggcttcc tgcgctcgca
     9301 cacgatcagc tgcgcgcttg tcatcgccag cgacgccgac cccggccatg gcatgagcga
     9361 gcacttcccg ttttcgccgg tgggtgcggc gctgttgtgc cgctggaccg acggcgacta
     9421 cggactcggc cgtgtcagct gggtcaacga gctcgacggc ggcgaaaact tccgtgccac
     9481 cgtgggttcc gacgacgcgc gcaacgtgtt gcgtttccac gagtcgcccg ccttcgacga
     9541 gcggctggcc actgcggcgg cgcacgcggt gcgcgactgc cttcagtccg agggactcac
     9601 gctctccgac gtcggcgccg tcgtcgccgc cccggcgcgg cagggttacc gcgccgccct
     9661 ggctcggcaa ctcgatgtgt catctgagcg ggtcatcgtc ggcgacgacg aacgcaccaa
     9721 caccgcagcg ctggcggccg ctatgcagcg cgcgatgaag gatgggccga tcgacgtgct
     9781 gctggtcgcc gctggtgccg gaatcacggc gggcgcggca ctgtatcgcg aaccgccggt
     9841 ctagcgccga gagcgacgtt ttgcaggccc gttctcgcac ttcttctgca agacgtcgct
     9901 ctcgtgaact tgtcggcggc acaggccaag atgccggcat gggggaggtc gttcgaggcg
     9961 gtgaggccgt cgcgacagga aagctcacac ggcacgagct gcagcggtgg tatcagccga
    10021 tctttcgggg cgtctacgta ccgaagcaca cgatgccgac tcttcgagac cgcatcgtgg
    10081 gtgcctggtt gtcgtcggga aggcaggcgg tcatcgcagg agtagctgcg tcggccttac
    10141 atggcgcaga gtgggtcgac gccgatgttg ctatcgaatt gatcacgcga atcagacccc
    10201 agcgcgggct cattgtccgt aaggggacac tcgccgaaga tgagtggacg tacatcggcc
    10261 ggcttcccgt caccacgccg gcccgcaccg cgttcgacct gggtaggcac ttgccgcgcg
    10321 ggcaagccct ggcgcggctc gacgcgctga tgcgcgcgac gccgttctcc atcgaagacg
    10381 tcctgctgct ggccaagcgc tacccgggcg cgcgcggcgt gcgccgattg cgcgaattac
    10441 taccgcttgt cgacggcggc gcgatgtcac cccaagagac gcggctgcgg ctactgttca
    10501 tcgacgccgg cttcccgaag cccaccacgc agattcctgt cgtcgacgaa cgaggccgcc
    10561 acgtacgcac actcgatatg gggtgggagg agttcatggt cgccgccgaa tacgacgggg
    10621 atcagcatca gatcaatcgc gcgcagtacg tgaaggatct ccaactgctg ccaaagctcg
    10681 aacgcctgcg ctggatcgtc gtgcgcgtca tcaaagaaga ctacgaagac gccatcctcg
    10741 accgcgccta ccaggccatg gtctcccgcg gctgggccgg acggttacgt cgaaagcgac
    10801 gtcttgcagg ccccttctcg cacgtcttct gcaaaacgtc gctctcggcg gaatggcttg
    10861 cgtgcgttag acgatgtcct ccggctcggg ggacgtctca aagatccgct cggcgtcgct
    10921 gcgatggcac ggcgctgtgc cgggcgtcat gagcatcgcc gatccggtgg cgatgccgaa
    10981 gcgcaccgac ttgcccatcg accatccccg ggtgaggcct accgtgatcg ccgccaccat
    11041 cgcgtcaccg gcgccgaccc cgctgaagac cttcacgggc accggcgaaa accgttgact
    11101 gccatgcgga gtggcgagca gcgcgccttt ggagcccagc gacaccacca cggtctgcgt
    11161 gatcccggcg tcgatgagtt cgtgggcggc ggccagttgg tcggcctcgc tgaccagatc
    11221 acggcccatg cgctcgcgta gttcccggac gctgggtttg agcaggaaca ccccggacgt
    11281 gatgtggcgc aagccgccgc cggagctgtc gaggaccagc agcacgccga gctcacggca
    11341 catgtcggcc acccgctggt agaagtcggc cggaacgccc gggggaaggc tgccgctggc
    11401 caccacgaac tccgcggacc gtgccgcgtc gcgcagctcg tcgagacatc gtgcttgttc
    11461 gacgaaggac aagcgagggc cgggaagcac gaaccggtac tgctgaccgc tgcattgctc
    11521 gtcgatcgtg aagctctccc gggtggactc ggtgatgtcg acgcggcgat acggaacgtc
    11581 ggcctccgcg accagctgtg tgaccaggtc accggtgtgt ccgcccgcgg ggaacaccgc
    11641 cgacaccgac ccgccgagca cacgagcgat ccgcgcgacg ttgataccgc caccgccggc
    11701 gtcgtaacgc acaccgcggc aacggatctt gtccgtcggg ccgacacggt ccgcgcttgt
    11761 cgtgacgtcg agcgccgggt tcatggtgag cgtgatgatc gttgttccgt actggatgtg
    11821 ctcgcctggg gatcccatga gtcggtggct ctccttctct cggcgtgaac ccgcggcgct
    11881 acaggcccgt cgggtacgtc tccggcgtct caccttcgac ccacaggctg gtcacctcga
    11941 gcggctcgag cgcggacgtg cggtcgatgt gaatgatcgc gtcgaactga tcggcgggcc
    12001 ggttgtggaa gtagtgactc tgccgctcgg tggcgggcag gtagatgacc ccgatcgccc
    12061 ggcccaaccg gaccaccgac agcggctcgg cggcggcgct ggacagcatc ggcgacacca
    12121 agaaggacgg attggccgtc tcatggaaca gctcctcgac gctgccgttc aacgccgtcc
    12181 ggacgacctt gcgctcggcg ggcccgcccc agtcgctggc tgcggtgacc gcgccgctgt
    12241 aggtggtgaa cccgatcaac cgcgtgtcac cgccgaactt ctcgcgggcg agttggccca
    12301 gagtgagctg accgtcggcc cccatctcgg tagcgcgtgc gtcgccgacg tgagagttgt
    12361 gcgcccacac cacgattcgg gccggcttgc cgtccggatg ccggtcgagg tgggtgagca
    12421 gagcatccag cgtttgcgcc atgtgggtgt cgcgcagatt ccacgacgtg acccgtccgc
    12481 tgaacatcga gcggtagtag acctcggcgt cgcggaccgt gacggcgttc tgctgcgcgt
    12541 agaacagctc gtcatcggcc agcaggccat cgcggcgggc gtaggcgagt gcgttgcgct
    12601 gcatctcgac caactgctcg atcgcctgct tctcacacga caagccggcg ccgaacgccg
    12661 ccgcgaaacc gtaggcctga ccgtcgtcgg ctgacgcgtg gtcgaagcac gcgtatcgct
    12721 tccgcgcgcg ctctgccgcc acggggtcga cgttgtccag gtaggcgatc acctcctgca
    12781 tcgaccggtg caggctgtag aggtccaaac cgtagaagcc gcactgccgt tctgcaccgc
    12841 gcccttcgtt gtaccggcgc agccagccga cgaagtcgcg gaccaccgtg ttgcgccaca
    12901 tccacgccgg gaagcgctcg aatccgcgca gcgcctcctc tggggtggtg tcgtcaccca
    12961 acccccgcac gtaccggttg acccggtagg cgtccggcca gtcggcctcc gccgtgacgg
    13021 cgcagaaccc cttctgctcg atcagccact tggtgatcgc tgcgcgggct tcgtagaact
    13081 cgtgcgtgcc gtgcgagctc tcgccgatca gcacgatgcg ggcgtcgccg atcagttcct
    13141 ccaacgcctc cgtgggcggc accccgcctg gggcgtccac ggcgacgtgg gcgatcactt
    13201 gggctggcgt ctctgcggcc ttcacggcag gtcttgccac cccggtggtc ggcgtggcca
    13261 gtagctcgca aacctcctcg tcgctggtct gactgaaatt ccagaacgac tcgccgacag
    13321 cgagaaacgg cgtcggcata ctcgcgcaca cgacgtcgtc gaccaggccg gcgaactccc
    13381 gaacggtcga ctccggcgcg gcgggcaccg cgatgacgat ctccgccggg tgggcttcgc
    13441 gcagtgcctg caccgccgcg aacatgctgg cgccggtggc cagcccgtcg tcgacgagga
    13501 tgacggtctt cccggtcacg tcgaccgggt ggcggccacc gcggtacgcg gtttctcgcc
    13561 ggatcagttc ccgcccttca cgttcggcca cgtcacgcaa ctgctggggg gtgatcccga
    13621 gtccccgaac tacatcgtcg ttgatgacga cgcgcccgtc gctggccagc gccccgaccg
    13681 cgaattcctc gtggcccgga gcgccgagct tgcgaaccac gaacgcgtcc aacggtgcgc
    13741 cgagtgccgc cgccacttcc catgccaccg gaattccgcc gcggggcaga ccgagaacga
    13801 cgacgtcgtc cttgccgcgg tacgccgtta gcagctgtgc gagaatccgc ccggcctcac
    13861 ggcggtcgcg gaacacccgc cgtggggcgg acggcgctga tcgcgtcgtt cgagtcattg
    13921 ctggctccct ttcagtgcag gttcgtcacc ggctcgtggg tcaccgcctg gatgtcggac
    13981 gccgtcacga caagcagttg atgtccgagg tccgacaggg cccgcgccgt cgcgagttcg
    14041 tcgccgatct cggccacgtt gtagtcggcc ggattccggc gggccactcc tatcccgacg
    14101 tagtccctgc cccgccattc cagccgggcc ttggctcggg tcttgttctc gtgttcgtcg
    14161 atctcgacct ggaccgtcca cgacttgacc tgatcgccgc tatgactcat ggcgcctcac
    14221 ttcgacgact cgatctcgat cttcttctcg gtcttcttgg gttcggatac cccgaccgac
    14281 accgtcagga tgcccttgtc gtaggtcgcc ttgatgtcgt cttcgtcggc tccctgaggc
    14341 agcgtgaccg tgcgctcgaa cgatccgtac gagaactccg agcggccgcc gctttcgctt
    14401 ttctccgtcc gctcggcttt gatcgtgagc cgtccgtcgt gcaccgtgat gtcgacgtcc
    14461 ttggccgggt cgacgccggg aatctcggcg cgcacctcat agcggccgtc tttggtcatc
    14521 tcgtcttcca gccgcatcag gcgggcgtcg aaaaccggac ggacgttggc aaacgacgga
    14581 aaccccgcga acaggtcggc gagctccggg aagagcgaac gctgctgacg cctttcgagg
    14641 ttgctcattg actcctcctt cggtcgaact agtggcacca tgatccgacc actcggccgg
    14701 gcccggcggt agggtctcaa gtccctgttt accgagcttt ggtctagccg cggccggcca
    14761 cgagatgcgt gacgaaccag tctcgcgcca gttccgcgac tttttcgagg gtgccgggtt
    14821 cctcgaacag gtgggtggcg ccggggacga ccgcgagtcg gcactctacc ggaatcgctt
    14881 gctgtgcttg ccgattcatc tcgaggacgg tttcgtcgcg tccgccgacg atcaacagag
    14941 tcggcgcatc gacgcgggcc agccactcgc cggccagatc gggcctgccg ccgcgcgaca
    15001 ccaccgcccc gacgctgacc cgcggatcgg cggccgcgcg cagcgccgcg ccggcgcccg
    15061 tgctggcccc gaagaagccc accggcaacg aggcggtgtc ggcctgggtg gcaagccatt
    15121 cggtgacgtc gaccaaccgg cgcgccagca gttcgatgtt gaacacattg gcgcggtcgg
    15181 tttcttcagc cggcgtcaga aggtcgaaca acaacgtgcc gaacccagcc gcgttcagca
    15241 cgtcggcgac gaagatgttg cgcgggctga acctgctgct tccgctgccg tgcgcgaaca
    15301 cgacgagccc tttcgggttg accgggatcg tcaggtgacc ggccaccgag accggccccg
    15361 ccgcgatctc gacttcctcg tcacgcagtg gcggtgtcca cttcgcgcgg cctgaatcgg
    15421 taaccatgca agcctcccga ttgtctgggg cgcagcctat atcggggccg gcgtttgttt
    15481 aagtgaccaa tgactcctga ccgacggcgg tccggcccct aatgtcatcg acagagtgct
    15541 catcagaagg agccgcgaaa tgaccgggcc cgtcacgcat cagggaatcc tcgtcggagt
    15601 cgacggatcc gcaccttcga acgccgcagt gcgatgggca gcccaagaag ccgccacgcg
    15661 caatctgccg ctgaccctgg tccacgcgcg aaagacgccg acggccggga caacagagat
    15721 ggcatggccc gcggtgcctc tccccgaaag gctgacgcgg tggcaggaag aggaagccca
    15781 gcaggtactc gccgactcgg tccaagtcgc cgaaggcgaa ctcacgagct cgaggctcaa
    15841 caccaaagtg atcttctcgg caccaatccc gacgctcatc gacctgtcca aggacgccgc
    15901 catggtcgtc gtgggctgtc gggggcaggg cgccctggcc cggacgctgt tgggatcggt
    15961 cagcaccgcc ctggtccacc acgcgcactg cccggttgcg gtgatccact acgcggttcc
    16021 ggcgcggtcg gagtggccgg tgctggtggg catcgacggt tcgccggcat cggagctcgc
    16081 cacagccatc gcgttcgacg aggcgtcatg gcgtggtgcg aatttggtgg ccctgcacgc
    16141 gtggaacgac gcggacgcgt acgccgtgcc gagcctggag tggtcagctt tggaagtggc
    16201 cgccgacaag accttagccg agcggctggc cggctgggag gaacgctacc ccgacgtcgc
    16261 ggtccggcgc gtggtcgtat gggagcaccc ggcgcgtcat ctgatcgagc gctccgaggt
    16321 gtcccaactg gtggtcgtgg gtagtcacgg acgaggcggc ttcgccggaa tgctgctggg
    16381 ctcggtcagc acggcggtgg tccacaacgc ccacaccccg gtgatcgtcg ctcggcagaa
    16441 ctaaccttcc gacgacgcgt accgcagtag ggcggcgaca ccgtcgaggc tgtcgacccg
    16501 aaccaacgaa gcgccgacgg caatcgcggc gaacggtagc gcctcgtccg cccgcgcaac
    16561 ccgaaccgct gcttgacccc actcagacag cacgtcggca cttggcgcca ctgcggcgcg
    16621 gtcctcaccg gtgaccaccg tcgcgtcggc gagttcgccg acgatcagcg tgtcgacgtc
    16681 gcccgcccgc agcgccgcgc aaacgggccc caggccctcg gcggcgagac cggatccgcg
    16741 gccgcgttcg gcctggaacc gctccatgac gtctgcggtc tcggcacgtc ggcgccggtc
    16801 gaactcggcg tcgacgaggc cgccgatcgc gttgtcgtcg atatcgcggc tgtgcacacc
    16861 ggcattcacc tgatgcacca gttgcgcgac ccgtgcgggc aacgccgaca ccacgtcggt
    16921 gcgggagcgg acctcaccgg atacgaacac cacctcgggg ctggtgtcgc gcaccaggtc
    16981 ggtgaggttc tcggcgaccg cgcggacgtt cttgcggatg gcttcctcga cccgcggttg
    17041 ggggtgggcg aactcgccct ccgccgcggg ggacttgtgc accgggtagc cgccgccgtc
    17101 gacagtgtgc tttcggacga cgtcaccttg atgcaggatg acgtctgcgc cggcatggtc
    17161 gaccgcggcc acgatgtatg tggggcgcca cgagccgaat tccaccagcg gcaggaggta
    17221 cggatagtcg gagatccgca gcgtggtcac cggcgggggt tccgtgagtt gctcgttgac
    17281 gaacgtctct tctgcggtcg cgatgatgcc ccggccgcgt cgcccgacgg cgggccgacg
    17341 gtgcagtacc gcgtcttcga gtcgggagat cacggcctgg tccgcacccg catcctcgag
    17401 atgcttgtag agatcgcgcc atctcgcgtt gagctgcgcc aacgcgtcgg cggtatcgtg
    17461 cgagtcgtcg aagtacaccg atacgaacgg gcctcgcgca gtcagcaacg ggtggaaacg
    17521 gtctgatcgc atcatcacac ccattcgccg cggaccacga tcaccggaac tgccgcggct
    17581 tgcgctaccg ccgagctcac cgaaccgagc agcatgctgc ggaatccgcc ccggccgcgg
    17641 ctgcccacga cgaccagctg ggcgtgctcg gactcacgaa tcagccagcg cgcaggctga
    17701 tcgcagacca ggcgccgctg caccgcgacg tcgggatact gctcgtgcca gccggccagc
    17761 cgttcgccca ggacctcgtg tccctcgctc tcatactccc gccagtccat gccgagcatc
    17821 gggaagactg ccacgtcgct ccacgcgtgc agcacgttga ggccgacgcc gcgacgcgat
    17881 gcttcgtcga aggccagcgc ggtggcggct tccgaggcgg gcgatccgtc gatgcccagc
    17941 agaatcgggg cgtcggggga gggctgtcgg ccggcgcgga tcaccgcgac cggacagcgc
    18001 gcgtggtgca gcagtccggc gctgaccgag cccagcagca tccggcccag cgcccccgtg
    18061 ccctggcttc cgacgacgat catctgcttg tcctcggatg cctcgacgag tgccggcacg
    18121 atggccgacc gcagaacgcc ggtgtgaacg tcgggtagct ccgactgggc ggcacaggat
    18181 tgaacggtct tgaccgcctg gtcgagcacc tgcccagcgt tgtcctcctg ccattcggtg
    18241 atgctggctt gcaccggccc gatgggccag gcagtgacca tgggctccag agcatggacc
    18301 agagtcaagg gtgcccggcg catcaccgcc tcggtggtgg cccagcggat cgcggcgtcg
    18361 gactccgcgg agccgtcgac accaaccaaa atgccggtcc tgcttgtgga ttgggccatc
    18421 tcactctcct caatccgcga gttcgatctt caaataacgg tcgatcccga agcgggcaaa
    18481 cgcgcgtcgc cgcctggggt acaactgggt ggcggccgcc tccgcgtcga tgacgaccgg
    18541 gtggacaacc tggtaggtct ggccacccgc ctggatggtt gcacggtcgg cggccagcac
    18601 gtcgcgaagc cagtcgacgc cggttccgta cggcagcggc acgatgaacc caccggccac
    18661 ccggtcggca accaccggcg tggcgtattc cttaccggtg cgacggccgg tatgccgaat
    18721 cacaccggcg taccagtgtt ttcgccccgc catgcgcatc atgaccgggt tgagtgcgtg
    18781 cttgttgaaa atgcgcaccg catcgcgagc ccaggtcatc ggctctcatg cctgatcgat
    18841 gactgcaccg ac
//
LOCUS       DASWBF010000268        11813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1212044,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000268 DASWBF010000000
VERSION     DASWBF010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212044"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /gene="rapZ"
                     /locus_tag="VF874_09855"
     CDS             <1..609
                     /gene="rapZ"
                     /locus_tag="VF874_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX9176365.1"
                     /translation="RVLFLEASDDVLVRRYEHVRRSHPLQGDQTLAEGIAAERRMLAP
                     LREGADLIIDTSTLPVRGLRESIERAFGGEAVAHTSVTVESFGFKYGLPMDADMVMDV
                     RFLPNPHWVDELRPQTGQSPAVRDYVLSQPGAEEFLDTYDRLLSLVVEGYRREGKRYM
                     TVAIGCTGGKHRSVAMAEALAGRLQPNDKLSVRVLHRDLGRE"
     gene            606..1646
                     /gene="yvcK"
                     /locus_tag="VF874_09860"
     CDS             606..1646
                     /gene="yvcK"
                     /locus_tag="VF874_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEX9176366.1"
                     /translation="MSYRIVALGGGHGLYATLSAARRLTPHVTAVVTVADDGGSSGRL
                     RNELDVVPPGDLRMALAALASDSGYGRLWATILQHRFGGSGALAGHPIGNLLLAGLNE
                     VLADPVAALDELGRILGVKGRVLPMCPIALQIEADVAGLESDSRMSRVIRGQVAIATT
                     PGKVRRVRLIPGDPPATRQAVDAILDADLVVLGPGSWFTSVIPHVLVPGLATALQATS
                     ARRALVFNLVAEPGETAGFSAERHLHVLSQHAPGFRVDEIVVDAERVPSDRERQQLIR
                     TASLLEAEVRFADVARPGTSLHDPAKLAAVLDSVRVRPATLPSAPPVPTRAVAPVEGS
                     SVNAPGGDDPWR"
     gene            1643..2620
                     /gene="whiA"
                     /locus_tag="VF874_09865"
     CDS             1643..2620
                     /gene="whiA"
                     /locus_tag="VF874_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX9176367.1"
                     /translation="MTAEVKDELSRLIVNSVSARKAEVSALLRFAGGLHIVAGRVIVE
                     AEVDLGIIARRLRKDIFDLYGYNAVVHVLSASGIRKQTRYVVRVAKDGEALARQTGLL
                     DLRGRPVRGLPAHVVGGSVGDAEAAWRGAFLAHGSLTEPGRSSALEVSCPGPEAALAL
                     VGAARRLGVSAKAREVRGADRVVVRDGDAIGVLLTRMGAQDTRLTWEERRMRREVRAT
                     ANRLANFDDANLRRSARAAVAAAARVERALQIIGDTVPDHLAAAGKLRVEHRQASLEE
                     LGRMADPPMTKDAVAGRIRRLLSMADRKAKQEGIPDTESAVTPDLLEDA"
     gene            2620..3465
                     /locus_tag="VF874_09870"
     CDS             2620..3465
                     /locus_tag="VF874_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9176368.1"
                     /translation="MKQYRRGDLTFDYIDDGPADGPVVVLLHGFPQLNTSWNAVIDRL
                     TARGYRCIAPNQRGYSPGARPKRRRDYRAQELVEDVRTLLDAVGAERVHLVGHDWGAA
                     VAWGAADELSDRVATLTSFSVPHPAAFTKALVTSRQGLASWYMYFFQLPWLPERLLLG
                     ADGKGVRAIKSLQSGGQTHSAAERDVKAMAEPGALTAALNWYRAMFLSNPRRGVGKIS
                     VPTMYVWSDGDTALTDKPARDTAAYVSGEYRFEILHGVSHWIPDEVPDKAADLLLEWF
                     DAHPI"
     gene            complement(3462..3863)
                     /locus_tag="VF874_09875"
                     /pseudo
     CDS             complement(3462..3863)
                     /locus_tag="VF874_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723627.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(4197..4595)
                     /locus_tag="VF874_09880"
     CDS             complement(4197..4595)
                     /locus_tag="VF874_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176369.1"
                     /translation="MNLKKFAAGATIAGALGAAALGFGAGSAQAKPHPGPPIPPIPVI
                     PVAPADWVPGDPPGHNPFGPPGQVMQGNPVVPGLTGVPPGHWGEPWLYGLPATWLPPI
                     PGVTEPLPVLWDPDVGAWGVWWNDVFTPFT"
     gene            5002..6024
                     /gene="gap"
                     /locus_tag="VF874_09885"
     CDS             5002..6024
                     /gene="gap"
                     /locus_tag="VF874_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9176370.1"
                     /translation="MTIRIGINGFGRIGRNLYRALEAQKAEGKSTDLEIVAVNDLTDN
                     ATLAHLLKFDSILGRLPQDVSLEGDDTIVVGSNKIKALEIKDGPAAMPWGDLGVDVVI
                     ESTGRYTEAAKAKGHLEAGAKKVIISAPAKGEDLTVVLGVNDDQYDGSQNIISNASCT
                     TNCLAPVTKVLNDEFGIVTGLMTTVHAYTQDQNLQDGPHKDLRRARAAALNIVPTSTG
                     AAKAIGLVMPELKGKLDGYALRVPIPTGSATDLTVELSKTASAEEINAAFKAAAEGRL
                     KGILKYYDAPIVSSDIVTDPHSSIFDSGLTKAIGNQAKVVSWYDNEWGYSNRLIDLAA
                     LVGKSL"
     gene            6027..7262
                     /locus_tag="VF874_09890"
     CDS             6027..7262
                     /locus_tag="VF874_09890"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9176371.1"
                     /translation="MADLPNLDDLLAQGVSGRGVLVRSDLNVPLDDDGHITDQGRIVA
                     SVPTLSALADAGAKVVVTAHLGRPKGAPDPKFSLKPVAAALAEKLGRHVQLAGDVVGP
                     DALARAEGLTDGDVLLLENVRFDPRETSKDDAERLALARQLAELVAPDGAFVSDGFGV
                     VHRKQASVYDVATLLPHYAGKLVEAEVKVLQQLTSSTERPYAVVLGGSKVSDKLAVIE
                     QLAQKADSLVIGGGMCFTFLAANGFSVGTSLVQPEMIDTCREILETHGDVIHLPVDIV
                     VADEFKADSPSETVVADRIPDGKMGLDVGPETVKRFTALLSNAQTIFWNGPMGVFEFP
                     AFAAGTKGVAEAIIGATGKGALSVVGGGDSAAAVRALGLPEDGFSHISTGGGASLEYL
                     EGKTLPGIQVLTTTSQGET"
     gene            7259..8056
                     /gene="tpiA"
                     /locus_tag="VF874_09895"
     CDS             7259..8056
                     /gene="tpiA"
                     /locus_tag="VF874_09895"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9176372.1"
                     /translation="MSRKPLIAGNWKMNLNHFEAIALVQKIAFSLPEKYYDKVDVTVL
                     PPFTDLRSVQTLVDGDKLKLTYGGQDPSQHDSGAYTGEISGAFLHKLGCTYVVVGHSE
                     RRTYHDESDELVAAKAAAAFRHALIPIVCIGEHLDVREAGNHVEHNIEQLRGSLAGLT
                     KEQIGQAVIAYEPVWAIGTGRVASSTDAQEMCKAIRDELANLASQQVADGVRVLYGGS
                     ANAKNIGDIVAQDDVDGALVGGASLDGEQFATMSAIAAGGPLPDLPG"
     gene            8076..8309
                     /gene="secG"
                     /locus_tag="VF874_09900"
     CDS             8076..8309
                     /gene="secG"
                     /locus_tag="VF874_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX9176373.1"
                     /translation="MELTLQILLVVTSILLVLLVLLHRAKGGGLSTLFGGGVQSSLSG
                     STAVEKNLDRVTLFVTAIWVISIVGVGLMIKYS"
     gene            8447..11236
                     /gene="ppc"
                     /locus_tag="VF874_09905"
     CDS             8447..11236
                     /gene="ppc"
                     /locus_tag="VF874_09905"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX9176374.1"
                     /translation="MAEDAALEPIGAVRRTRVGREATEPMREDIRLLGTILGDTVREQ
                     NGEEVFDLIERARVESFRVRRSEIDRAELAGMFDGIDIHEALPVIRAFSHFALLANVA
                     EDIHRERRRAIHVQAGEPPQNSSLAATYLKLDAAQLDSATVADALKDALVSPVITAHP
                     TETRRRTVFDTQHRITELMRLRLHGYSETPDGRDIETELRRQILTLWQTALIRLQRLK
                     IQDEIEVGLRYYHSAFFEVMPQVNAEVRHALQALWPDAHLFEHAILRPGSWIGGDRDG
                     NPNVTADVVRLATGSAAHTAFGHYFAELTALEEDLSMSARLVKVTDDLAALADRCHEQ
                     ARFDEPYRRALRVIRGRLTATAQDILDRQPLLELNLGLERYNTPAELLAELNVLDASL
                     RSHGGELLAGDRLARLRESVRVFGFHLSGLDMRQNSDVHEDVVAELLDWAGVHSDYTS
                     LSEPDRVELLAAELATRRPLTREHTQLSELAQKELDIVAAAARAVEVFGPDAVPIFII
                     SMCRSVSDMLEAAVLLKEVGLLDASGDEACSPVGIVPLFETIDDLQRGASILEAALDL
                     PVYRSLVRARGDSQEVMLGYSDSNKDGGYLSANWALYRAELDLVETTRKAGIRLRLFH
                     GRGGTVGRGGGPSYDAILAQPPGAVSGSLRLTEQGEVIAAKYAEPRIARRNLETLVAA
                     TLESTLLDVEGLGDDAEPAYQVLDELAALAQHAYAELVHETPGFVDYFTGSTPVSEIG
                     ALNIGSRPTSRKQTTSISDLRAIPWVLAWSQSRVMLPGWYGTGSAVEQWIADGDDRLE
                     VLQDLYRRWPFFSTVLSNMAQVLAKSDMGLAAHYAELVPDEELRRRVFEKIVDEHERT
                     IRMHQLITEQEDLLADNPALARSVFNRFPYLEPLNHLQVELLRRYRSGDDDELVQRGI
                     LLTMNGLASALRNSG"
     gene            11267..11722
                     /locus_tag="VF874_09910"
     CDS             11267..11722
                     /locus_tag="VF874_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9176375.1"
                     /translation="MRVSAKAEYAIRAMVELATTDGAALTKADDVAKAQGIPSQFLLD
                     ILMDLRRDRLVRSRRGPGGGYELARPATEISVADVLRCIDGPLISVSGGGVGELPYVG
                     PTAALTDVWTALRESMRTVLEQTSLADIAADNLPEHVRTLAEDYRDSEG"
BASE COUNT         2027 a         3845 c         4026 g         1915 t
ORIGIN      
        1 cgggtgctgt tcttggaggc ctccgacgac gtcctcgtcc gacgctacga gcatgtgcgg
       61 cgaagccatc cgctgcaggg cgaccagaca ctggccgaag gaatcgcggc cgagcgcagg
      121 atgctggcgc cgctgcgcga aggcgccgat ctgatcatcg acacctcgac gctgccggtg
      181 cgggggctgc gcgaaagcat cgagcgggcg ttcggcggtg aggccgtcgc acacaccagc
      241 gtcacggtcg aatctttcgg gttcaaatac gggcttccga tggacgccga catggtgatg
      301 gacgtacggt tcctgccgaa cccgcactgg gtcgacgaac tacgacctca gaccggccag
      361 tcgccggcgg tgcgtgacta cgtgttgtcc caacccggtg ccgaagaatt cctcgacacc
      421 tacgatcgac tgctatccct cgttgtcgag ggctatcgac gggaggggaa gcgctacatg
      481 accgtcgcca tcggctgcac cgggggcaaa caccgcagtg tcgcgatggc tgaggcgttg
      541 gccggtcggc tgcaaccgaa cgacaagctg tcggtgcgcg tactgcaccg ggacttgggc
      601 cgcgaatgag ctaccgtatc gtcgccctcg gcggtggcca tgggctgtat gccacgttgt
      661 ccgccgcgcg gcggctcacc ccgcatgtca ccgcggtggt cacggtcgcc gatgacgggg
      721 gttcctcggg gcggctgcgc aacgagctcg acgtcgtgcc gccgggcgat ctgcgaatgg
      781 cgttggcggc gttggcttct gacagcgggt acgggcggct gtgggccacc atcctccagc
      841 accgattcgg cggaagcggt gcgctggccg gccacccgat cggcaatttg ttgctcgccg
      901 ggctcaacga ggtgctcgcc gaccccgtgg ccgcactgga cgagctcggc cggattctcg
      961 gcgtcaaggg ccgcgtgctg ccgatgtgcc cgatcgcgtt gcagatcgaa gccgacgtcg
     1021 ccgggctcga gtccgactcg aggatgagcc gcgtgattcg cgggcaggtg gcgatcgcga
     1081 cgacgcccgg taaggtgcga cgggtccggc tgatcccggg cgatccgccg gccacgcgtc
     1141 aggccgtaga cgcgatcttg gacgcggatc tagttgtttt gggcccgggt tcgtggttca
     1201 caagcgtgat accccacgtc ctggttccgg gtctggccac agcgctgcag gcgacgtccg
     1261 cgcgccgtgc gctggtgttc aacctggtcg ccgagccggg ggagaccgcc ggcttctccg
     1321 cggagcgcca tctgcacgtg ctgtcgcagc atgcccccgg tttccgggtg gacgagatcg
     1381 tcgtcgacgc cgagcgcgtg cccagtgacc gcgagcgaca gcaattgatc cgcaccgcga
     1441 gcctgcttga ggcggaagtg aggtttgctg acgtggcacg gcctggtaca tctctacatg
     1501 acccggcgaa gttggccgcg gtcttggaca gcgtgcgagt ccgcccagcg acgctgccga
     1561 gcgcgccacc ggtgccgacc cgcgcggtcg cgccagtgga gggatcgagt gtgaatgcac
     1621 cgggaggtga cgacccgtgg cgatgaccgc cgaggtcaag gatgagttga gcaggttgat
     1681 cgtcaactcg gttagcgccc gcaaggcgga agtgtcggcg ctgctgcgct tcgccggcgg
     1741 actgcacatt gtggccggtc gcgtcatcgt cgaggccgag gtggacctcg gaatcattgc
     1801 ccggcggctg cggaaggaca tcttcgacct gtacggctac aacgccgtcg tgcatgtgtt
     1861 gtccgccagc ggaattcgca aacaaacccg ttatgtggtg cgagtggcca aggacggcga
     1921 ggcgctggcg cggcagaccg ggctgctgga tctgcgcggc aggcccgtgc gcgggctgcc
     1981 ggcccacgtc gtcggcggca gcgtgggcga cgcggaggcg gcctggcgtg gcgcgttcct
     2041 ggcgcatggg tcgttgaccg aaccggggcg atcgtcggca ctggaggtga gctgtccggg
     2101 accggaggcg gcgctggcac tggtcggggc ggcgaggcga ctgggcgtca gcgccaaggc
     2161 gcgcgaggtg cgcggggccg accgtgtcgt ggtccgcgac ggcgacgcga tcggcgtgct
     2221 gctgacccga atgggggcac aggacacccg gttgacgtgg gaggagcggc ggatgcgccg
     2281 cgaggtccgc gcgacggcca accggctggc gaacttcgac gacgcgaact tgcgacgctc
     2341 ggcgcgggca gcggtggcgg cggcggcacg cgtcgagcgg gcgctgcaga tcatcggcga
     2401 cacggtgccc gatcacctgg ccgcggcggg caagctgcgc gtcgagcatc gccaggcgtc
     2461 gctggaggag ctgggccgga tggccgatcc gccaatgacg aaagatgctg tggcaggccg
     2521 gattaggcgg ctgctgtcga tggccgaccg gaaggcgaag caggagggca tccccgacac
     2581 ggagtcggcg gtgaccccgg acctactcga ggacgcgtag tgaagcaata ccgccgtggc
     2641 gacctgactt tcgactacat cgatgatggg cccgccgacg ggccggtcgt cgtgttgttg
     2701 cacggctttc ctcagctcaa cacgagctgg aacgcggtca tcgaccggtt gacggcacga
     2761 gggtaccgat gcatcgcgcc gaaccagcgt ggctattcac cgggcgcccg cccgaagcgg
     2821 cgccgcgact accgcgctca ggaactcgtc gaggacgtcc gcacgctgct cgacgcggtc
     2881 ggtgctgagc gggtgcacct ggtcggacac gactggggcg ccgcggtcgc gtggggagca
     2941 gccgacgaac tcagcgaccg cgtcgcgacg ctgacgtcgt tctcggtgcc tcatccggcc
     3001 gcgttcacca aggcgctcgt gacgagccgg caaggtctcg cgtcctggta catgtacttc
     3061 ttccagctgc cgtggcttcc ggagcggctg ttgctcgggg cggatggcaa gggcgtccgg
     3121 gcgatcaagt ccctgcagtc cggcggccag acgcattcag ccgccgagcg cgatgtcaag
     3181 gcgatggccg agccgggtgc gctgaccgcc gccctcaatt ggtatcgcgc aatgttcttg
     3241 agcaacccgc gccgtggggt cggaaagatt tccgtgccga cgatgtacgt ctggagcgac
     3301 ggggacaccg cgctgaccga caaaccggca cgcgataccg ccgcgtacgt cagcggggaa
     3361 taccggttcg agatcctgca cggcgtctcg cactggattc cggacgaagt acccgataag
     3421 gcagccgacc tactgctgga gtggttcgac gcccacccga tctagaacgg cggttcgtcg
     3481 ggtaggggcg gcggtttggg cggtggctgc cagtccggcg gcagctcttc gggttcaatc
     3541 gggtcattga cccatcggca gaacgggctg gtgctagatt tacctttgaa gagaaataac
     3601 atgcgccgca tgtgatttcg gaacttgcgc gcttcgattt cgtcttttcg ggctcgcccg
     3661 acgcggcggt gctcttcgtt gaggggccgt tgggtgcggt tttgcttgcg cgcgtgggca
     3721 attcgtgtcg cccgggcccg actgtggttg cgccgctgcc gtttcggggc ggcgcaggga
     3781 ggaccggtga aacccggaag cagatccgct gcgccgggtg tggtgcgata gaacttaccg
     3841 gtgggcgacg tccagatcac ggtccgtctg gaattattct gggatacagg ttgaccgtga
     3901 gcttccacga tcgcggcggg tgatcggtat gggcgccgaa cttacgccgg caatggccac
     3961 tgccgagcag ccgcgccaac cactcgaacc gctccccgga cccagtacgt gcctcacaaa
     4021 cccggcactt acaaatcgtt ctcgtgtttt cgctaccgag gtcggtcggt acgcatgccg
     4081 ctcccgtccc tatctcgact ttgcgagcca tcctcgatcc cgaggattcg ttctgtgagg
     4141 cgaagaaggc caccctgcct cggcagggtg gccttcttgt tttgtcgagt cgtctactac
     4201 gtgaacggag tgaacacgtc attccaccag acgccccagg ccccgacatc ggggtcccac
     4261 aagactggta acggctccgt gacgccagga atcggcggga gccacgtcgc gggcaagcca
     4321 taaagccaag gttctcccca atgccccggc gggactccag tgagtccggg aactaccggg
     4381 tttccttgca tcacctgtcc gggcgggccg aacgggttgt gcccgggcgg atctcccgga
     4441 acccaatccg ccggagccac cggaatcacc ggaatcggcg gtatcggagg ccccggatgc
     4501 ggtttcgcct gcgctgaacc tgcgccgaat ccaagggcgg cagcgcccag cgcgccggcg
     4561 atggtggcgc ccgcagcaaa tttcttcaga ttcatagtgg actccttgtc cagcgatctt
     4621 gcgctgccct cggtggctgc gccagcgtcg actcgttgtt catgcagatt tcttaatgta
     4681 gccccaccgc ccttgctgac ccggcttcgg caatgacccc ccgatgatcc ctggataccc
     4741 caccgcgaac aggcctaaaa cctcttcgtt cggccgaaat ccagacgagc tcgccttcga
     4801 cgcaccagtg gtagtcgtcg cgccgccagc gttgggtgtg cccggtcgca accaaaatcc
     4861 tcatgctccc acctcacaag tcctacgagc cgacgttaaa agcgacccac tgacatctcg
     4921 ggcgacgcga gtgggtgcag ccgacaacac agcactaggc tggcctcaag agagcagacc
     4981 aaggcagacg aggagaaacc agtgacgatc cggattggca tcaacggctt cggtcgaatc
     5041 ggacgtaact tgtaccgggc gctcgaggcg cagaaggccg agggcaagag caccgacctc
     5101 gagatcgtgg cggtcaacga cctcaccgac aacgccaccc tggcgcacct gctgaagttc
     5161 gactcgatcc tggggcggtt gccgcaggac gtcagcctcg agggcgacga cacgatcgtg
     5221 gtcggcagca acaagatcaa ggccctggaa atcaaagacg gcccggccgc gatgccgtgg
     5281 ggcgatctcg gtgtcgacgt ggtcatcgag tccaccggtc gatacaccga ggccgccaag
     5341 gccaagggcc acctcgaggc gggcgccaag aaggtcatca tttccgcccc ggccaagggc
     5401 gaagacctga ccgtcgtgct cggcgtcaac gacgaccagt acgacggcag ccagaacatc
     5461 atcagcaacg cctcctgcac caccaactgc cttgcgccag tgaccaaagt gctcaacgac
     5521 gagttcggca tcgtcacagg cctgatgacc acggtccacg cgtacaccca ggaccagaat
     5581 ctgcaggacg gtccgcacaa agacctgcgc cgcgcccgcg ccgccgcgct gaatatcgtg
     5641 ccgacgtcga ccggcgccgc gaaggccatc ggcctggtga tgccggagct gaaaggcaag
     5701 ctcgacggct acgcgctgcg ggtgccgatc cccaccggct cggccaccga cttgaccgtc
     5761 gagctgtcga agacggccag cgccgaagag atcaacgccg cgttcaaggc cgccgccgag
     5821 ggcaggctga aaggcatcct gaagtattac gacgcgccga tcgtctccag tgacatcgtc
     5881 accgacccgc acagctcgat cttcgactcc ggcctgacca aggcgatcgg caaccaggcc
     5941 aaggtggtgt cctggtacga caacgagtgg ggttactcga atcggctcat cgacctcgcc
     6001 gcattggtgg gcaagtcgct gtagacatgg ccgacctgcc gaatctcgac gatcttctcg
     6061 cgcaaggggt ttcgggacgc ggagtgctcg tgcgctcgga cctcaacgtc ccgctcgacg
     6121 acgacggtca catcaccgac cagggtcgca tcgtcgcgtc ggttccgacg ctcagtgcgt
     6181 tggccgacgc cggcgccaag gtcgtggtca ccgcccacct cggccggccc aagggcgctc
     6241 ccgatccgaa gttctcgctc aagcccgtcg ccgccgccct cgccgaaaag ctcggccgcc
     6301 acgtgcaatt ggccggcgac gtcgtcgggc ccgacgcgct tgcccgagcc gagggcttga
     6361 ccgacggtga cgtcctgctg ctggagaacg tccggttcga cccgagggaa accagcaagg
     6421 acgacgccga acggctggcg ctggcaaggc aattggccga actggtagca cctgacggcg
     6481 cgttcgtgtc cgacgggttc ggtgtcgtgc accgcaagca ggcctcggtg tacgacgtcg
     6541 cgacgttgct gccacactac gcgggcaagc tggtcgaggc ggaagtcaaa gtgctgcaac
     6601 agttgaccag ttccaccgag cggccgtacg cggtcgtgct gggcggatcc aaggtgtccg
     6661 acaagctggc ggtgatcgaa caactggcgc agaaagccga cagcctggtc atcggtggcg
     6721 ggatgtgttt caccttcctt gccgcaaacg ggttttcggt gggcacatcg ctcgtgcagc
     6781 ctgagatgat cgacacctgc agggaaattc tggaaaccca cggcgacgtg atccacctgc
     6841 cggtggacat cgtggtggcc gacgagttca aggccgattc gccgtcggaa accgtggtcg
     6901 ccgaccggat acccgacggc aaaatgggtt tggacgtcgg accggagacg gtgaagaggt
     6961 tcaccgcgct tctgtcgaac gcgcagacga tcttctggaa cggcccgatg ggtgtgttcg
     7021 agttcccggc gttcgcggcc ggcacgaaag gcgtcgccga ggcgatcatc ggcgccacgg
     7081 gcaagggcgc gttgagcgtg gtcggcggag gtgattcggc cgccgcggtg cgcgcgttgg
     7141 gcctgcccga ggacggcttc tcgcacattt cgaccggtgg cggagcttcg ctggaatacc
     7201 ttgagggcaa gacactgccc ggcattcaag tgctgaccac caccagccaa ggagagacgt
     7261 gagccgcaag ccgctgatcg ctggcaactg gaagatgaac ctcaaccact tcgaagcgat
     7321 cgcgctggtg cagaagattg cgttctcgct gccggagaag tactacgaca aggtcgacgt
     7381 caccgtgctc ccgccgttca ccgacctgcg ctcggtgcag acgctggtcg acggcgacaa
     7441 actgaagctc acctacggcg gccaggaccc ctcgcagcac gacagcggcg cctacacggg
     7501 cgagatcagc ggcgctttcc tccacaagct cggctgcacc tacgtcgtgg tcggccattc
     7561 cgagaggcgg acatatcacg acgagagcga cgagttggtc gccgcgaagg cggccgcggc
     7621 gttccggcac gcgctcatcc caatcgtctg catcggcgag catctcgacg tgcgcgaggc
     7681 cggcaaccac gtcgagcaca acatcgagca gctgagggga tccttggcgg gcttgaccaa
     7741 ggagcagatc ggccaggcgg tgatcgccta cgaaccggtg tgggccatcg gcacgggccg
     7801 ggtggccagc tcgaccgacg ctcaagagat gtgcaaggcc atccgcgacg agctggccaa
     7861 cctggcatcg cagcaggtcg ccgacggcgt gcgggtcctt tacggcgggt cggccaacgc
     7921 caagaacatc ggcgacatcg tggcccagga cgacgtcgac ggcgccctgg tcggcggtgc
     7981 gtcgctggac ggcgagcagt tcgcgacgat gtcggcgatc gccgccgggg ggccgctgcc
     8041 cgacctgccc ggttagggcc ggtagggtag cgcccatgga gttgaccctg cagatcctgc
     8101 ttgtggtcac cagcatcctt ctggtgttgc tggtgctgct gcatcgcgcc aagggcggcg
     8161 ggctgtcgac gctgttcggc ggcggggttc agtccagcct gtccggctcg acggcggtcg
     8221 agaagaacct cgaccgcgtg acgctgttcg tgaccgcaat ctgggtgatc tcgatcgtcg
     8281 gcgtcgggct gatgatcaag tacagctgac gcctgcaccc gccagccctc cagacttaaa
     8341 cgtctgcggc cgtcgtcggc gcgtcgccgc agagctttgg gtgtcgcggc gcaccgaccg
     8401 ccgcttccgc catgcgcggc gccccaagcg caccgatact tggctcatgg ctgaggacgc
     8461 ggcgctggag ccgatcggcg ccgtccggcg cacccgggtg ggacgcgagg cgaccgagcc
     8521 gatgcgcgag gacatccggc tgctcggcac catcctcggc gacacagtcc gcgagcagaa
     8581 cggcgaggag gtgttcgacc tcattgaacg cgcccgggtc gaatcgtttc gggtgcggcg
     8641 ctcggagatc gaccgcgccg agctagccgg gatgttcgac ggtatcgaca tccacgaagc
     8701 ccttccggtg atccgggcgt tcagccactt cgcgctgctg gccaacgtcg ccgaagacat
     8761 ccaccgggaa cggcgccgcg caatccacgt ccaggctggc gagccacccc agaacagcag
     8821 cctggccgcc acctacttga agctcgatgc agcacaactg gattcggcca ccgtcgccga
     8881 cgcgctcaaa gacgcgctgg tgtcaccggt gatcacagcg caccccaccg agacccgacg
     8941 ccgcaccgtc ttcgacaccc agcaccggat caccgagctg atgcggctgc ggctgcacgg
     9001 gtacagcgaa actcccgacg gccgcgacat cgaaaccgag ttgcgccgcc agatcctgac
     9061 gctctggcag accgcgctga tccggttgca gcgcttgaag attcaagacg agatcgaagt
     9121 gggcctgcgg tactaccact cggcgttctt cgaggtgatg ccccaagtca acgccgaagt
     9181 ccgccacgcg ctgcaggcgc tctggcctga cgcgcacctg ttcgagcacg cgattctgcg
     9241 gcctgggtcg tggatcggcg gagaccgcga cggcaacccg aacgtgaccg ccgacgtcgt
     9301 gcggctggcc accggcagcg cggcgcacac cgcgttcggg cactacttcg ccgagctgac
     9361 cgcgctggag gaagacctgt ccatgtcggc gcgactggtc aaggtcaccg acgacctcgc
     9421 cgcgctggcc gaccggtgcc acgaacaggc ccggttcgac gagccgtacc ggcgcgcgct
     9481 gcgggtcatt cgcgggcggc tcaccgcgac cgcacaggac atcctggacc gccagcccct
     9541 actcgagctc aacctgggtc ttgagaggta caacacaccc gccgaactgc tcgccgagct
     9601 caacgtcctc gacgcgtcgc tgcgaagtca cggtggcgag ctgctcgccg gcgaccggtt
     9661 ggcgcggctg cgagagtcgg tgcgagtctt cgggttccac ctgtccgggc tcgacatgcg
     9721 gcagaactcc gacgtgcacg aggacgtggt cgccgagttg ttggactggg ccggtgtgca
     9781 ttcggattac acgtcgctct cggaaccgga ccgtgtcgag ctgttggctg ccgaactggc
     9841 cacccggcgt ccgctgacgc gggagcacac acaactctct gagttggccc agaaggaact
     9901 cgacatcgtc gccgccgcag cgcgggcggt cgaggtgttc ggcccggacg cggtgccgat
     9961 cttcatcatc tcgatgtgcc gctcggtgtc cgacatgctc gaggccgcgg tcctgctgaa
    10021 ggaggtcggc ctgctggacg cttcgggtga cgaggcgtgc tcgccggtgg gcatcgtgcc
    10081 gctgttcgag acgatcgacg acctgcagcg cggagcgtcg attctcgaag cggcgctgga
    10141 ccttccggtg taccggtcgc tcgtccgtgc acgcggcgac agccaagagg tgatgctcgg
    10201 ttactcggat tccaacaagg acggcggcta cctgtcggcg aactgggcgc tgtaccgcgc
    10261 cgaactcgac ctcgtcgaaa ccacgcgcaa ggccggtatt cggttgcggc tcttccacgg
    10321 tcgcggcgga accgtcggcc gcggcggcgg accgagttac gacgccatcc tcgcgcaacc
    10381 gccgggcgcg gtgtccgggt cgttgcggct caccgagcag ggcgaggtca tcgccgccaa
    10441 gtacgccgag ccgcgcatcg cccggcgcaa tctcgagacg ctggtggcgg ccacgttgga
    10501 gtccacgctg ctcgacgtgg agggactcgg cgacgacgcc gaacccgcct accaggtact
    10561 tgacgaactg gccgcactgg cacagcacgc gtacgccgaa ttagtccacg aaacaccggg
    10621 attcgtcgac tatttcaccg gctcgacccc ggtcagcgag atcggcgcgc tcaacatcgg
    10681 cagccgcccg acgtcgcgca agcagaccac gtcgatctcc gatctgcgcg cgatcccctg
    10741 ggtgctggcg tggagccagt cacgcgtgat gctgcccggc tggtacggaa ccggcagcgc
    10801 ggtcgagcag tggatcgccg acggggatga tcgcctcgag gtgctgcagg atctctaccg
    10861 gcgctggccg ttcttctcga ccgtgctgtc gaacatggct caggtgctgg ccaagtccga
    10921 catggggttg gcggcgcact acgcggagct ggttcccgac gaggagttgc gccgtcgggt
    10981 gttcgagaag atcgtcgacg aacacgagcg cacgatcagg atgcaccagc tgatcaccga
    11041 gcaggaggac ctgctcgccg acaatccggc gctggcacgg tcggtgttca accgcttccc
    11101 gtacctggag ccgctcaacc acctgcaggt ggagctgctg cgccgatacc gctcgggtga
    11161 cgacgacgag ctcgtgcagc gcgggatcct gctgaccatg aacggactcg cgtctgcgct
    11221 gcgcaacagc ggatagggtc aagtcgctcg gtggcaaaat ctcggtgtgc gcgtgtcagc
    11281 gaaggccgaa tacgccatcc gggcgatggt cgagttggcg acgacggacg gggccgcgct
    11341 gacgaaggcg gacgacgtgg ccaaagccca ggggatcccg tcccagttcc tgctggacat
    11401 tctcatggac ctgcgtcggg atcggttggt gcgcagccgc cgcggccccg gcgggggata
    11461 cgaactcgcg cgtccggcga ccgagatcag cgtcgccgac gtgctgcgct gcatcgacgg
    11521 cccgctcatc agtgtgtccg gcggcggtgt gggggaactg ccgtacgtcg gtccgaccgc
    11581 agcgttgacg gatgtgtgga cggcgctgcg tgagagtatg cgcacggtcc tcgaacagac
    11641 ctcgctggcc gacatcgctg ccgacaacct tcccgagcac gtccgcacgc ttgccgagga
    11701 ctaccgcgac agcgaaggat aattcccggc taatccagcc gacaatatgc gtcgcggtta
    11761 tcagactcgg aaacaggccg tttgcctagg gcgtgacccc ccgtgatgca tca
//
LOCUS       DASWBF010000269         4476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_228766,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000269 DASWBF010000000
VERSION     DASWBF010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228766"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VF874_09915"
     CDS             complement(<1..249)
                     /locus_tag="VF874_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176376.1"
                     /translation="MTAVVAQPEGALAEIEELGYTVVKGVLDPASVAAFLEDTNRLAR
                     ELPTVISNSTTVVKGFARPGQAPVTGSDHDWVRIDNLLL"
     gene            complement(490..1608)
                     /locus_tag="VF874_09920"
     CDS             complement(490..1608)
                     /locus_tag="VF874_09920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEX9176377.1"
                     /translation="MPLLSRLSARVGDVPPSTSGRSRHDPLLALGYAILSGVWGLTGS
                     VRRPPPVPERDRTHTLRVAQRRIVAHDEDVVALTLAAADGKPLPRWFPGAHVDIHLPS
                     GRVRQYSLCGDPKSRDSYRIAVRRVANGGGGSIEVHDGLTVGATVTTGGPRNAFPLSI
                     PGYGSPTQRFRFIAGGIGITPILSMLRRAQQLGVDWSMIYVGRSAGSLPFVDEVSAFG
                     EAVEIRTDDVHGLLTADDLLGECPDGTAVYACGPAPMLTAVRARLAGRDDVELHFERF
                     AAPPVVDGEEFSVCIASTGTTVAVGAEDTLLSALQRAGVAAPYSCQQGFYGTCRTQVL
                     SGDVEHRDTLLTEPERAAGMMLTCVSRAHTGERLTLDL"
     gene            complement(1608..2507)
                     /locus_tag="VF874_09925"
     CDS             complement(1608..2507)
                     /locus_tag="VF874_09925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX9176378.1"
                     /translation="MLQPQRFEHEVDPGPVQIQPRKVHFDFSGIPLHWIPGHPVASDM
                     IGILNVVLPAAERWFVATYNEALPLVKDPKLVDDMRGFIGQEAIHADVHERALHEFME
                     ARGFDPKPMVDQIEYAFSKILGPSASTNPRRRRNQLCDRLWFIAALEHYTAVLGDFSL
                     NNKWDDAGAHPTFVDLFRWHGAEEVEHRNVAHDVATYFSDSYLDRVGAMLMASTFMFV
                     MFQRVLWHLVKTDPGVEFGWLKMQRLRYLDSKNRLLPRYRKMFGSNTLDYLRPGFTPD
                     QMGSTAQAVAYLASSPAARAAHL"
     gene            2572..2803
                     /locus_tag="VF874_09930"
                     /pseudo
     CDS             2572..2803
                     /locus_tag="VF874_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891920.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
     gene            complement(2815..3021)
                     /locus_tag="VF874_09935"
     CDS             complement(2815..3021)
                     /locus_tag="VF874_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176379.1"
                     /translation="MSGELTRWRLNLDDDSTITFDEATQTITKVSTGETCDLGDYPSF
                     AKEGATLFVSSGHAPKLGKWYVPG"
     gene            3108..3527
                     /locus_tag="VF874_09940"
     CDS             3108..3527
                     /locus_tag="VF874_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEX9176380.1"
                     /translation="MPKTTKRGSAKKSELPSTLKKSPAKAQRTFAKVHDAAVDQYGEG
                     ERAHRVAYDALKHSYEKVGDHWEPKSKKGPSDPRAKSGGPNPTGKSAEGVNATASKKH
                     LMDVARRLDISGRSTMNKDELVSAIKKANRRESARNR"
     gene            complement(3524..4476)
                     /locus_tag="VF874_09945"
                     /pseudo
     CDS             complement(3524..4476)
                     /locus_tag="VF874_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          809 a         1442 c         1525 g          700 t
ORIGIN      
        1 aagcagcagg ttgtcgatac gtacccagtc gtgatcggag ccggttaccg gcgcctggcc
       61 aggccgagcg aaccccttga cgacggtggt ggagtttgag atcaccgtgg gcaactctcg
      121 agcaagccga ttggtgtcct ccaggaacgc cgccaccgac gccgggtcga gcacgccttt
      181 cacgacggtg tagccgagct cttcgatctc cgctagcgca ccttcgggtt gcgcgaccac
      241 ggcggtcatg agggcagcag atcgatgtcg gcgccactcc ggtcgcaatc gtccacatac
      301 gtggcgtctt cgaaatcctt cacgatgctt ttcctttcgt ttgtcatcaa actggtgtgc
      361 ccgcggtggg cacatagcga atatcgcgga acatgcgcgc caggtcagtc ggctgacggg
      421 ctgatgatgc ggatgaattc gatgtccgcc gattggggga cgcccgcttt tcgcggggcg
      481 acgacggtgc tacaggtcga gagtgaggcg ttcgccggta tgggcgcgcg acacgcaggt
      541 cagcatcatc ccggccgcgc gttcgggctc ggtgagcagc gtgtcgcggt gctcgacgtc
      601 gccgctgaga acctgcgttc ggcaggtgcc gtagaatccc tgctggcatg aatacggcgc
      661 cgccactcct gcccgctgca gcgccgacag cagcgtatcc tccgcgccga cagcgacggt
      721 cgtgccggtg gacgcgatgc acaccgagaa ctcctcgccg tcgacgacgg gcggcgcggc
      781 gaaccgctcg aaatgcagtt cgacgtcgtc gcggcctgcc agtcgggcgc ggacagcggt
      841 cagcatcggc gctggcccgc acgcgtaaac ggcggtgccg tcgggacatt cgcccagaag
      901 gtcgtccgcg gtcagcagcc cgtggacgtc gtcggtgcgg atctcgactg cctcaccgaa
      961 cgctgacacc tcgtcgacga acggcagact tccggcgctg cgcccgacgt agatcatcga
     1021 ccagtcgacg cccaactgct gcgcgcggcg aagcatcgac aggatcgggg tgattccgat
     1081 accgcccgca atgaaacgga agcgctgcgt gggggagccg tagccgggaa tcgacagcgg
     1141 gaacgcgttg cgggggccgc ccgtcgtcac cgtggcgccg accgtgagcc cgtcgtgcac
     1201 ctcgatcgag ccaccgccgc cattggctac gcgccgcacc gcaattcggt acgagtcacg
     1261 acttttgggg tcgccgcaca gcgagtactg ccggacccgc ccgctgggca ggtggatgtc
     1321 gacgtgcgcg cccgggaacc accgcggcaa cggctttccg tccgcggcag ccaaagtcag
     1381 cgccaccacg tcctcgtcgt gggccactat ccgacgctgc gccacccgaa gcgtgtgcgt
     1441 gcggtcgcgc tccggcaccg gcggtgggcg gcggaccgaa ccggtgagtc cccacacgcc
     1501 ggacaggatc gcgtagccca acgccagaag cgggtcgtgg cgggaccggc ccgaggtgct
     1561 cggcggtacg tcgccgaccc tggcggaaag ccttgacagc aacggcatca cagatgcgcg
     1621 gcacgcgcgg ccggtgaact cgccaggtaa gcgaccgcct gtgcggtcga ccccatctgg
     1681 tccggagtga accccggccg gagatagtca agggtgttgc tgccgaacat ctttcgatag
     1741 cggggcagga ggcggttctt cgagtcgagg tagcgcagcc gctgcatctt caaccacccg
     1801 aattccaccc cggggtcggt cttcacgaga tgccacagga cgcgctggaa catcacgaac
     1861 atgaacgtcg acgccatcag catggcgccg acgcggtcga ggtagctgtc gctgaagtag
     1921 gtggcgacgt cgtgggcgac gttgcggtgc tcgacctcct cggcgccgtg ccagcggaac
     1981 aggtcgacga acgtcggatg tgcgccggca tcgtcccact tgttgttcaa cgagaagtcg
     2041 ccgagcaccg cggtgtagtg ctcgagcgcg gcgatgaacc acagccggtc gcacagctgg
     2101 ttgcgccggc gcctcgggtt ggtggacgcc gacgggccga ggatcttgga gaacgcgtat
     2161 tcgatctggt cgaccattgg cttgggatca aagccgcgcg cctccatgaa ctcgtgcagc
     2221 gcccgttcgt ggacgtcggc gtgaatggct tcctggccga tgaagccgcg catgtcgtcg
     2281 acgagtttgg gatccttgac caggggcagc gcttcgttgt aggtggcgac gaaccagcgt
     2341 tccgccgcgg gcagcaccac gttgaggatc ccgatcatgt ccgaggcgac cgggtggccc
     2401 gggatccagt gcagcgggat gccggagaag tcgaagtgca cttttcgcgg ctggatctgc
     2461 accgggcccg ggtcgacttc atgctcgaat cgttgcggtt gcagcatgga ggaagtctac
     2521 ggccgaacgt cgtcaggttt cgtttgcccg cgggctgtca atatgtcggg aatggccgga
     2581 atcggcgatg tgctggagtg ggcgcgccac cagctggtgt cacgcgtccc gaaggcggat
     2641 ctcgaccagc gcgatcccga ctacatccgt gaccagctgc cgggtttgtg gttgctggcg
     2701 tcggtgtact tccggaccga cgtgcgcggc ctgggaccgc atccccgcag aggatccggt
     2761 gctgctggtg ggcaaccaca gcggcggcaa cgtggcaccg gagctggtcg agtcctagcc
     2821 gggcacgtac cacttgccca gcttgggggc gtggccgctg ctgacgaaca gcgttgcgcc
     2881 ctctttggcg aacgacgggt agtcgccgag gtcacaggtc tcgcccgtgc tgaccttcgt
     2941 aatcgtctgc gtcgcttcgt cgaatgtgat tgtgctgtcg tcgtcgaggt tcaatcgcca
     3001 ccgggtcagt tcaccgctca tgacgcagga cgctaccgcg ccggaatcct ctagttttcg
     3061 cgaagtcggt gcggggtaga cgtgatcgtc atcaaaggag ttcgacgatg cccaaaacca
     3121 cgaagcgcgg atcggcgaag aagagcgagc tgccgagcac cctgaagaag tcgccggcca
     3181 aggcacagcg gacattcgcg aaggttcacg acgcggccgt agatcaatac ggcgagggtg
     3241 aacgcgcaca ccgcgtcgcc tacgacgcgc tcaagcacag ctatgagaag gtcggcgacc
     3301 actgggagcc caagagcaag aagggcccgt ccgacccgcg cgcgaagagc ggcggaccga
     3361 acccgacggg gaagtcggcc gagggtgtga acgcgaccgc cagcaagaag catctgatgg
     3421 atgtggctcg gcggctggat atctcagggc ggtcgacgat gaacaaggac gaactggtgt
     3481 cggcgatcaa gaaggccaac cggcgcgagt cggcgcgcaa ccgctagtgc ggcggctcga
     3541 acccctccag taaatagcgc agctgcgccg gggtgagcgc ccgtcgggtc ttccgccggg
     3601 taatcaagat gccacgacgg cgagcgccca cacgccgtat tggacggtcc acgcggcgcg
     3661 gaacgagtcg aacgagtagc cccctgcggc tccgatgatg aggcccatcg tctgcatcac
     3721 gattagcgac gcgaggaacc cgcccatgtt gaccatgccc tgcgcggttc ccagcgtcgc
     3781 gcgcgggttg aacgtgcgcg cgaagtcgaa gcccaccatc gaccctgggc cgtcgacgga
     3841 gatctccacg accagcagcg tgagaagcca cagcggagcc cggtgcggca gcgccagcac
     3901 aaccgtccag atcgccgcgt tgctcgcgat gatggtcagc accatccacg accggcgcat
     3961 cgggtagcgg gccgtcagca cgccgatcac gatgcccgcg acgatggccg tcaccaccga
     4021 gagcgacagc agcgcgcctg ctgtgccctt cgaaacaccc tgcgcggcag tcaggtacgg
     4081 gacaccccac atcaacgcaa agactgtgac ggaaaactgg gttcccatgt gcgtgaagaa
     4141 gcccagccgc gtacccggac acagccacac ggtctttacg ctggcgagca tggcgcgcag
     4201 gtcggtcttt tcgtcgcggg ggaccgcgcc ttcgggggtg tcgtgcacga gggagagcgt
     4261 caacgcgatc gacagcagcc ccagcgcggc caccgacgta taggcgacgg tccaaccgcc
     4321 gtcactgacg agcgcgaacc tgcgcgcccg cgtacaccac gacttgcagc acgacgaacg
     4381 acgacagcac gcccgggctg gccccgaacc ggttcgcggc ctccaggccg gacacgccca
     4441 gcgtggtgcg gtcgaggacg gcgacgatgt aggcga
//
LOCUS       DASWBF010000270         6293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_153925,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000270 DASWBF010000000
VERSION     DASWBF010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153925"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VF874_09950"
     CDS             <1..805
                     /locus_tag="VF874_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX9176381.1"
                     /translation="VDGHVSVIDTAVGSPNRDTVVGHVELGEPPAALRIAPNGRWAYA
                     INHFDHAVWAVHTATNRVATIRLLAYPYRISLSPNGSRLYASLSDDGSICVIDTDPAS
                     ATYHLIIARIRLGHGPEPVFTTAGTRAYVVKSDENSVAVINTADSSVKDIAVGQYPWD
                     LAVSPDGRRAYVANSLDDSLSVISCATDCVTATIPVGAHPCRVAVCATGDRAFVVNSA
                     DDSVSVIDTATATVRRIIAVGRTPCDVAVSADGLRAFVQHRDGLSLVSL"
     gene            919..2556
                     /locus_tag="VF874_09955"
     CDS             919..2556
                     /locus_tag="VF874_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX9176382.1"
                     /translation="MSRFTATMCRNALTSSNGFVTGEPHAPVRQSWHEVHHRARRVAG
                     GLASAGVGHGDAVGVLAGAPVEIAPIAQGVWMRGASLTMLHQPTPRTDLALWAQDTMT
                     VIDMINAKVVIVSDPFLAAIPVLEERGVKVVTVEQLLAADPIDPVDTDEDDVALMQLT
                     SGSTGSPKAVVITHRNLHSNAEAMFVGAEHDLDHDVIVSWLPLFHDMGMTGFLVVPMY
                     FGAELVVVTPMDFLRDILLWARLIDKYKGTMTAAPNFAYALLAKRLRKQAKPGEFDLS
                     TLRWALSGSEPVEPADVEDLIDAGKPFGLRPDAILPAYGMAETTVAVSFSECGAGMVV
                     DEVDADLLAVLRRAVPSTKGHTTRLASLGPLLKGLEVRIVDEDANLLPPRGVGVIELR
                     GEPLTRGYVTMGGFVPAQDEQGWYDTGDLGYLTEEGHIVVCGRVKDVIIMAGRSVYPT
                     DVERAASRVDGVRPGCAIAVRLNAGHARESFGVAVESNAWDDPAEVRRIKHAVAHAVV
                     AEVDVRPRNVVVLSPGTIPKTPSGKLRRAHALSLVSC"
     gene            2773..5151
                     /locus_tag="VF874_09960"
     CDS             2773..5151
                     /locus_tag="VF874_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX9176383.1"
                     /translation="MGVRRRFTGRDVTRDVEVVYDEHDVTATGAERESAGVKAVMVSM
                     QRALKSMGPLRTLASLARLNQRKGFDCPGCAWPEEPGGRRFAEFCENGAKAVAEEATK
                     RVTAPEFFARHSIADLETRPEFWLSQQGRLTHPMVLRDGDDHYRPIGWDDAYQLIAEH
                     LRALDSPDEAVFYTSGRTSNEAAFLYQLMVRSFGTNNLPDCSNMCHESSGHALIEAIG
                     VGKGSVSVTDVEAADLIIIAGQNPGTNHPRMLSVLEKAKANGAKIIAVNPLPEAGFFQ
                     FKDPQKVHGMIGNGIPIADEFVQIRLGGDMALFAGLGRLLLEADDNAPGSVVDRDFVA
                     EHCANFEAYEKHTRAVDLDTVLQATGIDRAQLDRVAQMLIASERTVACWAMGLTQHRH
                     AVPMISEITNVLLMRGMIGKPGAGLCPVRGHSNVQGDRTMGIWGRMPEKFLAALDRRF
                     GIVSPREHGVDTVGAIRAMRDGRAKVFMGMGGNFALATPDTPVTEAALRNCALTVQVS
                     TKLNRSHVVHGREALILPSLGRTDKDIQNGAKQQVSVEDSMSMVHLSRGSLQPPGPEV
                     RSEVAIVCQLARTLLGPDHPVPWEGFNDDYERIRDAIAAVVPGCDDYNRKVRWPDGFQ
                     LPHSPRDSREFPTSTGKANFAVNPLEWVPVPDGRLVLQTLRSHDQYNTTIYGLDDRHR
                     GVHGGRRVVFVNPADIDRFGLTAGARVDLVSEFTEADGHLQERRAKDFMVVAYSTPVG
                     NAAAYYPETNALVALDHVAAKSNTPVSKAVVIRLDAASGVERSERGNTQWGA"
     gene            5139..5963
                     /gene="fdhD"
                     /locus_tag="VF874_09965"
     CDS             5139..5963
                     /gene="fdhD"
                     /locus_tag="VF874_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX9176384.1"
                     /translation="MGRVTARRPAHHLTAGDIVSRPETLAVEEPLEIRVNGSPLTVTM
                     RTPGSDIELAQGFLLTEGVITGRDDVLTVRYCRGSGNDGLNTYNVLDVTLAEDVPAPD
                     VDITRNFYTTSSCGVCGKASVDAVRLISRHCPGDDPASVAADALTAMPGQLRSAQRVF
                     DSTGGLHGAALFAVDGTMLAVREDIGRHNAVDKVIGWALESGRIPLSGTVLLVSGRAS
                     FELTQKAVMAGVPVLAAVSAPSSLAVDLASQSGLTLVAFLRGESMNVYTRADRVNR"
     gene            6024..>6293
                     /locus_tag="VF874_09970"
     CDS             6024..>6293
                     /locus_tag="VF874_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176385.1"
                     /translation="MTELKTPTAVLHEGPRSLSEHETAEIERRDLHGIRTVDIDDVIE
                     YVHHGLRRLPDFVTLYRKYLKQRWDVYELDFTQDRIDWTEKMTEDE"
BASE COUNT         1070 a         2082 c         2146 g          995 t
ORIGIN      
        1 ggtggacggc cacgtgtcgg tgatagacac cgctgtcggc agcccgaatc gcgacactgt
       61 cgtcggacac gtcgaactcg gggagccgcc ggcagcgctg cggatcgcgc ccaatggcag
      121 atgggcgtac gccatcaacc acttcgacca cgcggtgtgg gccgtccaca ctgccacgaa
      181 cagggtggcg acgatccgac tgctggcgta tccataccga atctcgttga gcccgaacgg
      241 ctcacgactc tacgccagcc tcagcgatga cggatcgatc tgcgtgatcg acaccgaccc
      301 ggccagcgct acctaccacc tcatcatcgc tcgcatccgg ctgggccatg gtcccgaacc
      361 ggtcttcacc accgccggca cgcgcgccta tgtcgtcaag tccgacgaga actccgtagc
      421 cgtgatcaac accgcggaca gcagtgtcaa agacattgcc gtaggccagt atccgtggga
      481 tctggcggta agccccgatg gccgacgcgc atacgtggcg aactctctcg acgactcact
      541 gtcggtgatc agctgcgcaa cggactgcgt caccgcgacg attcccgtcg gcgcacaccc
      601 atgccgcgtc gcggtatgcg caaccggtga ccgggcgttc gtcgtcaaca gcgcggacga
      661 ctcggtgtcg gtgatcgaca ccgctaccgc gaccgtaagg aggataatcg cggtggggcg
      721 cacaccgtgt gatgtggccg tcagcgctga cggcctgcgg gcgttcgtcc agcaccgcga
      781 cgggttgtcg ctggtttcgc tctaaggctc acttcagctc gtggtcaaat cgccgaccca
      841 attggtcgcg gaattgcgct cgatgccgca tgcttgagcg ccagggagtg accagcacgc
      901 tcctgggagg ccgctcggat gagtcgattt accgccacga tgtgccggaa tgccctcaca
      961 agttcgaacg gatttgtcac cggcgagccg catgcaccgg tccggcagag ctggcacgag
     1021 gtgcaccacc gagcgcggcg ggtagccggt gggctcgcct cggccggtgt cggacacggc
     1081 gacgcggtcg gtgtgctcgc cggtgccccg gtcgagatcg cgccgatcgc ccagggcgtg
     1141 tggatgcgcg gcgccagcct gacgatgttg catcagccga cgcctcgcac cgatctggcg
     1201 ctgtgggcgc aggacaccat gacagtcatc gacatgatca acgccaaggt ggtgatcgtc
     1261 tccgacccgt tcttggctgc gatcccggtg ctcgaggagc gcggcgtcaa agtcgtcacc
     1321 gtggaacagc tgctggccgc cgatccgatc gaccccgtcg ataccgacga ggacgacgtc
     1381 gcgctgatgc aattaacgtc gggatctact gggtctccca aggccgtggt gatcacgcac
     1441 cgcaaccttc actccaacgc ggaggccatg ttcgtcggcg ccgagcacga cctcgatcac
     1501 gacgtgatcg tcagctggtt gcccctgttc cacgacatgg gcatgaccgg ctttctggtc
     1561 gttccgatgt acttcggtgc cgagctcgtc gtcgtcacgc cgatggactt cctgcgcgac
     1621 atcctgctgt gggccagatt gatcgacaag tacaagggca cgatgaccgc cgcgccgaac
     1681 ttcgcctacg cgcttctcgc caagcggctg cgcaagcagg ccaagcccgg ggagttcgac
     1741 ctgtccacac ttcggtgggc gctgtcgggc tccgagcctg tggagccggc cgacgtcgag
     1801 gatctgatcg acgcgggcaa gccgttcggg ttgcggccgg acgcgattct gccggcctac
     1861 gggatggcgg agacgacagt ggcggtctcg ttctcggagt gcggtgccgg catggtggtc
     1921 gacgaggtcg atgccgacct gctcgcagtg ctgcggcgcg ccgtgccctc gaccaagggc
     1981 cataccaccc gcctggcgtc actcgggcca ttgctgaagg ggctggaagt gcgcatcgtc
     2041 gacgaggacg cgaacttgct gcccccgcgt ggggtgggcg tcatcgagtt gcgcggcgag
     2101 ccgctgaccc gtggctacgt gacgatgggc ggtttcgtcc cggcgcagga cgagcagggc
     2161 tggtacgaca ccggcgacct gggctacttg accgaggagg gccacatcgt ggtctgcggc
     2221 cgcgtcaagg acgtcatcat catggccggc cgcagcgtct acccgacaga cgtggagcgg
     2281 gcggcgtccc gtgtcgacgg cgtccgtccc gggtgcgcaa tcgcggttcg gctcaacgcc
     2341 ggccatgccc gggagagctt cggggtcgcg gtcgagtcga acgcgtggga cgacccggcc
     2401 gaggtgcgcc ggatcaagca tgcggtcgcc cacgcagtgg tggccgaggt cgacgtgcgg
     2461 ccccgcaacg tcgtcgtgct cagcccgggg acgatcccga agacgccgtc gggcaagctc
     2521 cgccgcgctc acgcgctgtc gctggtgagt tgctgaacgc gcgctggctc atcggggatg
     2581 cccttgccgg caggggcccg cgcacctggt cgccgacacg cacggccgga tggtccccgg
     2641 gaaatacagc ggctgccgac cgtgcagaac ccgtacacgc tcggcgtcga ttcgaccacc
     2701 ggccggttgt tcgtcgccgg cgtcaccgac ggtgtggtgc agatcatcga accggcggcc
     2761 taaattagca ggatgggggt tcgccgccgg ttcacaggac gggatgtcac ccgcgacgtc
     2821 gaagtcgttt acgacgaaca cgacgtcacg gccacgggcg cagaacgcga atccgccggc
     2881 gtcaaagccg tgatggtgtc gatgcagcgc gcgctcaaat cgatggggcc gttgcgaact
     2941 ctggcgtcgt tggcacggct gaaccagcgc aaggggttcg actgtccggg atgcgcgtgg
     3001 cccgaggagc caggcggccg caggttcgcc gaattctgcg agaacggcgc caaggcggtc
     3061 gccgaggagg ccaccaagcg cgtcaccgcg ccggagttct tcgcccggca ctcgatcgcc
     3121 gacctggaga ccaggcccga gttttggctg agccagcagg gccggctcac ccatccgatg
     3181 gtgttgcggg acggggacga ccactaccgg ccgatcggct gggacgacgc gtaccagctg
     3241 atcgccgagc acctgcgcgc cctcgacagc ccggacgagg cggtgttcta cacctccggg
     3301 cgcaccagca acgaggccgc gttcctatat cagctgatgg tgcgcagctt cggcaccaac
     3361 aacctgccgg attgctccaa catgtgccac gagtcgtcgg gccacgcgtt gatcgaggcg
     3421 atcggcgtcg gcaagggatc ggtgagcgtc accgacgtcg aggcggccga cctcatcatc
     3481 atcgccggcc agaacccggg gaccaaccac ccccggatgc tgtccgtgct ggagaaggca
     3541 aaggccaacg gcgccaagat cattgcggtc aatccgcttc ccgaggcggg atttttccag
     3601 ttcaaggacc cgcagaaggt tcacggcatg atcggcaacg gcatcccgat cgccgatgag
     3661 ttcgtgcaga tccgcctggg cggcgacatg gcattgttcg ccgggttggg ccggctgctg
     3721 ctcgaggccg acgacaacgc ccccggcagc gtcgtcgacc gcgacttcgt cgccgagcac
     3781 tgcgccaatt tcgaggccta cgagaagcac acgcgggcag tcgatctcga cacggtgctg
     3841 caggcgacgg gcatcgaccg cgcccagctg gatcgggtcg cgcagatgct gatcgcgtcc
     3901 gagcgcaccg tcgcgtgctg ggcgatgggg ctgacgcagc accgccacgc ggtgccgatg
     3961 atctccgaga tcaccaacgt gcttctgatg cgcggcatga tcggcaagcc cggtgccgga
     4021 ttgtgcccgg tgcgcgggca ttccaacgtg cagggcgacc gcacgatggg catctggggg
     4081 cggatgccgg agaagttcct ggccgcgctc gaccgccggt tcgggatcgt cagcccgcgc
     4141 gaacacggcg tcgacacggt gggcgccatc cgcgcgatgc gcgacggccg cgcgaaggtg
     4201 ttcatgggca tgggcggcaa cttcgcgctg gcgaccccgg acacgccggt caccgaggct
     4261 gcgctgcgca actgcgctct gacagtccag gtttcgacga agctgaaccg cagccacgtc
     4321 gtgcacggcc gagaggcgct gatcctgccg tcgctgggcc gcaccgacaa ggacatccag
     4381 aacggcgcca aacagcaggt gtccgtggag gattcgatgt cgatggtgca cctgtcgcgg
     4441 ggcagcctgc aaccgccggg ccctgaggtg cgcagtgagg tcgcgatcgt ctgccagctc
     4501 gcacggaccc ttctcggccc ggatcatccc gttccgtggg aggggttcaa cgacgactac
     4561 gaacggatcc gcgacgcgat agccgcggtg gtgccgggct gcgacgacta caaccggaag
     4621 gtgcgctggc ccgacggctt ccagctgccg cactccccgc gtgactcgcg cgaattcccc
     4681 accagcacag gcaaagccaa cttcgcggtg aatccgctgg agtgggtgcc ggtgccggac
     4741 ggtcggctgg tgctgcagac gctgcgcagc cacgaccagt acaacaccac catctacggc
     4801 ctcgacgacc gccatcgcgg cgtgcatggc ggccgacgcg tggtgttcgt caacccggca
     4861 gacatcgaca ggttcgggct gacggcgggc gcgcgggtgg acctggtatc ggaattcacc
     4921 gaggccgacg gccacctcca ggagcgccgc gccaaggatt tcatggtagt ggcgtactcg
     4981 acgcccgtcg ggaacgccgc cgcctactat ccggagacca acgccctggt agcgctggat
     5041 catgttgcgg ccaagtcgaa cacgcctgtc tcgaaggcgg tggtgatccg cttggatgcg
     5101 gcctcggggg tcgagcgcag cgagcggggg aacacccagt ggggcgcgta acggcgcgcc
     5161 ggccggcgca ccacctgacg gcgggtgaca tcgtcagccg gcccgagacg ctggcggtcg
     5221 aagagccgct ggagattcgc gtgaacggtt cgccgctcac ggtcacgatg cggactcccg
     5281 gctccgatat cgaactggca caaggctttc tgctcaccga aggtgtgatc accggtcgcg
     5341 acgacgtgct gacggtgcgc tactgccgcg gatccggcaa tgacggcctg aacacctaca
     5401 acgtgctcga cgtgacgctg gccgaggacg tcccggcgcc cgacgtcgac atcacgcgga
     5461 acttctacac cacctcgtcg tgcggtgtct gcggcaaggc gtcggtggac gcggtgcggt
     5521 tgatcagccg gcactgcccc ggcgacgacc ccgcgagtgt cgccgccgac gcattgaccg
     5581 ctatgcccgg ccagttgcgg tccgcgcagc gggtcttcga cagcaccggc ggcctgcacg
     5641 gggccgcgct gttcgcggtg gacggcacca tgctggcagt tcgcgaagac atcggtcgcc
     5701 acaacgccgt ggacaaggtg atcggttggg cactggagtc cgggcgaatc ccgttgagcg
     5761 gcacggtgtt gctggtcagc ggccgcgcgt cgttcgagct cactcagaag gcggtgatgg
     5821 ccggggttcc ggttctggcc gcggtgtcgg cgccgtcgtc actggcggtc gacctggcca
     5881 gccagtcggg cctgaccttg gtggcgttcc tgcgaggcga gtcgatgaac gtctacaccc
     5941 gcgcggaccg ggtgaatcga tagaaaccgc tacaccttgc cagcgggctg gcgcaacatg
     6001 gtcgtacgag cggaaggttg gccatgaccg aactcaagac ccccacagcg gtgctgcacg
     6061 agggaccgcg cagcctttcg gagcatgaga ccgccgagat cgagcggcgc gatcttcacg
     6121 gcatccgcac cgtcgacatc gacgacgtca tcgagtacgt ccaccacggc ctgaggcgac
     6181 tgcccgactt cgtcaccctg taccgcaagt acctcaagca gcgctgggac gtctacgaac
     6241 tcgacttcac ccaggaccgc atcgactgga ccgagaagat gaccgaggac gaa
//
LOCUS       DASWBF010000271         6884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1212839,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000271 DASWBF010000000
VERSION     DASWBF010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212839"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VF874_09975"
     CDS             complement(<1..550)
                     /locus_tag="VF874_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9176386.1"
                     /translation="MRPAPLPVRDGLGPARLRLQGGSVVDEFRARFGPVAAAKVTAGE
                     VVDADGAVLDGRSVMPAGSFVYLYRELAREVPVPFDIPVLHRDDDIVVVDKPHFLATM
                     PRGRHVAQTATVRLRWALGLPELSSAHRLDRLTAGVLLFTTRREVRGRYQTLFSRGLV
                     RKTYLARSAGQSNTELPRVVHSR"
     gene            complement(547..2292)
                     /locus_tag="VF874_09980"
     CDS             complement(547..2292)
                     /locus_tag="VF874_09980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX9176387.1"
                     /translation="MIEAAPAPGNGQTFLGPREREVAWERLGSEQFDVVVIGGGVVGA
                     GAALDAATRGLKVALVEARDFASGTSSRSSKMFHGGLRYLEQLEFGLVREALHERELS
                     LTTLAPHLVKPLPFLFPLTRRWWERPYVAAGIFLYDQLGGSKSVPAQKHLFKAGALRL
                     APGLKRNSLIGGIRYYDTVVDDARHTMTVARTAAHYGAVVRASTQVVSLLREGDRVVG
                     VRARDSENGAAVEVRGHVVVNATGVWTDEIQALSKQRGRFRVRASKGVHIVVPRDRIV
                     SEVAIILRTERSVLFVIPWGTHWIIGTTDTEWNLDRAHPAATKADIDYLLGTVNTVLA
                     TPLTHDDIDGVYAGLRPLLAGESDDTSKLSREHAVAVPAPGLVAIAGGKYTTYRVMGE
                     DAIDAASEYIPARVAPSITEKVPLLGADGYFALVNQTERVGAYYGLHPYRVRHLLDRY
                     GSLIGEVLAMAEDKPDLLSPITDAPVYLKVEAVYGAVAEGALHLEDILTRRMRIGIEY
                     PHRGVDCAREVAEIVAPVLGWAEADIQREVDTYIAGVDAEVRSQQQPDDESADALRQA
                     APEARAEILEPVPLN"
     gene            complement(2336..3742)
                     /locus_tag="VF874_09985"
     CDS             complement(2336..3742)
                     /locus_tag="VF874_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HEX9176388.1"
                     /translation="MATRIVIIGGGPAGYEAALVAAAHPAEVTVIDSDGIGGACVLFD
                     CVPSKTFIASTGVRTDLRRAPQLGYDIDIEDAKISLPQIHDRVKTLAAAQSADIAERL
                     RSQGVRLISGRGELADPVPGMAQHRVEVTEPDGTEQILDADVVLIATGASPRELPDAR
                     PDGQRILNWRQLYDLTDLPDHLVVVGSGVTGAEFVNAYTELGVPVTVVASRDQILPHE
                     DSDAAAVLEEVFAERGVTLVKKARAQSVTRGDDGVVVTMTDGRCVEGSHALITVGSVP
                     NTSGLGLERVGIELGKGDYIPVDRVSRTSVPTIYAAGDCTGLLPLASVAAMQGRIAMY
                     HALGEGVTPIRLRTVAAAVFTRPEIAAVGVPQARIDDGTVPARTLNLPLNTNARAKMS
                     GLRKGFVKIFCRPATGVVIGGVVVAPVASELILPIALAVSNQASVADLAQTLSVYPSL
                     TGSITEAARRLMAHDDLD"
     gene            3810..4289
                     /locus_tag="VF874_09990"
     CDS             3810..4289
                     /locus_tag="VF874_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX9176389.1"
                     /translation="MPLYAAYGSNMHPEQMLQRAPHSPMAGTGWLHGWRLTFGGEDIG
                     WEGALATIVEDPNSKVFVVLYDMTKEDEKNLDRWEGSEFGVHKKIRCRVERVSSDTTT
                     DPVLAWLYVLDAWEGGVPSARYLGVMADAAEIAGAPADYVHALRTRPSRNIGPGHGD"
     gene            complement(4430..5602)
                     /locus_tag="VF874_09995"
     CDS             complement(4430..5602)
                     /locus_tag="VF874_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX9176390.1"
                     /translation="MSLADTAESWLAAHYDDLVAWRRHIHAHPELGRQEFATTQFVAT
                     LLAEAGLNPKVLPAGPGLTCDLGPDHLPKIALRADMDALPMPERTGAPYASVVPGVAH
                     ACGHDAHTAMLLGTALALATVPDLPVGVRLIFQPAEELMPGGAIDAIAAGVLAGVSRI
                     FALHCDPRLDVGRVAITAGPITSASDSIEIGLHSPGGHTARPHLTADLVYGIGTLITG
                     LPGVLSRRIDPRNSTVMVWGAVNAGAAPNAIPQSGSLAGTVRTASHDTWLTLESLVRE
                     TVASLLAPLGIEHSLQYRRGVPPVVNEEVSTRILTHAIEAVDPDALAETRQSGGGEDF
                     SWYLEEVPGAMARLGVWPGRGPQLDLHQPTFDLDERALAIGLRVLVNTIEQAAQFL"
     gene            complement(5599..6882)
                     /locus_tag="VF874_10000"
     CDS             complement(5599..6882)
                     /locus_tag="VF874_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014588776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX9176391.1"
                     /translation="MCSAIAAAARSASSEAVMLSELSLQCDTAMMPATSPPTRHAEAV
                     TRADVARDVVLSRRNDLVALSHDIHAEPELAFTEHRSCAKAQVLVAERGFAITTAPAG
                     LDTAFRADYGSGELTVAVCAEYDALPGIGHACGHNIIAASAVGAALALAEVADDVGLT
                     VVLMGTPAEESGGGKALLLKGGAFDDICAAVMVHPGPADIAAARSLALSEVNVHYRGH
                     ESHASVAPHLGVNAADAATVAQVAIGLLRQHLAPGQQVHGIVTEGGRAPNVIPGHAAM
                     RYSIRATDKESLKSLEKRVFECFHAGALATGGECRIEPSGPGYDELRPDPWLANVFRD
                     EMIRRGRSPMPAELETTLPLGSTDMGNVTHAMPGIHPVVGVDTGGASLHQPEFTAVAA
                     SPSGDDAVIDGAIMLARTVIRLAETPSERDRVISR"
BASE COUNT         1063 a         2436 c         2331 g         1054 t
ORIGIN      
        1 ttcgactatg gacgacccgc ggcagctccg tgttcgactg gcctgccgag cgcgccaggt
       61 acgtctttcg gaccagcccg cgtgagaaca gcgtctggta gcggccgcgc acctcgcgtc
      121 ttgtggtgaa caacaagaca cctgcggtca agcggtccag ccggtgcgcg gagctcagtt
      181 ccggcaggcc cagcgcccaa cgcaaccgca ccgtcgcggt ctgcgcgacg tgtcggcccc
      241 gcggcatggt cgcgaggaaa tgcggcttgt cgacgaccac aatgtcgtcg tcgcggtgca
      301 gcaccgggat gtcgaacggc accggcacct ctcgggccaa ttcacggtag aggtacacga
      361 aagagcccgc cggcatcacg ctgcggccgt cgagcacagc cccgtcggcg tcgacgacct
      421 cgccagccgt caccttggcc gcggccaccg gaccgaaccg ggcacggaac tcgtcgacca
      481 cggacccgcc ttgcagccgc aaccgtgcgg ggccgagccc gtcgcgcacg ggcaacgggg
      541 ccggcctcaa ttgagcggca ccggctcgag gatctccgcg cgggcctccg gggcggcttg
      601 ccgcagtgca tcggccgact cgtcgtccgg ctgctgctgc gatcgcacct ccgcgtccac
      661 cccggcgatg taggtgtcga cctcgcgctg gatgtcggct tcagcccagc ccagcaccgg
      721 cgccacgatc tcggcgacct cgcgggcgca gtccacgccg cgatgcgggt attcgatgcc
      781 gatccgcata cgtcgggtca ggatgtcctc gaggtgcagc gcgccctcgg ccaccgcccc
      841 gtataccgcc tccaccttca ggtacaccgg cgcatcggtg atcggactca gcaggtcggg
      901 cttgtcctcg gccatggcca ggacttcgcc gatcagcgag ccgtagcggt ccagcaggtg
      961 acgcacccga tacgggtgca ggccgtagta ggcacccacc cgctcggtct ggttgaccag
     1021 cgcgaagtag ccgtcggcgc ccagcagcgg caccttctcc gtgatcgacg gcgccacgcg
     1081 cgcagggatg tactcgctgg ccgcgtcgat cgcgtcctcg cccatcaccc ggtacgtcgt
     1141 gtacttaccg ccggcaatcg cgaccagccc cggcgccggc accgccaccg cgtgctcgcg
     1201 ggacagcttc gacgtgtcgt cgctctcccc ggccagcagc gggcgcagcc ccgcgtacac
     1261 gccgtcgatg tcgtcgtgcg tgagcggggt ggccagcacg gtgttcacgg tgccgaggag
     1321 gtagtcgatg tcggccttgg tggcggccgg gtgggcccgg tcgaggttcc actcggtgtc
     1381 agtggtgccg ataatccagt gcgtgcccca tggaatcacg aacagcaccg agcgctcggt
     1441 gcgcaggatg atcgcgacct cgctgacgat gcggtcgcgg ggcacgacga tgtgcacccc
     1501 cttggaggcc cggacccgga accggccgcg ctgcttggac aacgcttgga tctcgtcggt
     1561 ccacaccccg gtggcgttga cgacgacgtg gccgcgcacc tcgacggccg cgccgttctc
     1621 cgagtcacgc gcccgcacgc cgaccacccg gtcgccctcg cgcagcagcg acacgacttg
     1681 ggtcgaggcg cggaccacgg ccccgtagtg ggcggccgtg cgggcgaccg tcatcgtgtg
     1741 ccgggcgtcg tcgacgacgg tgtcgtagta ccggatgccg ccgatcagcg agttgcgctt
     1801 cagcccgggg gcgagccgca acgcgcccgc cttgaacaaa tgcttttgtg ccggaacgga
     1861 tttcgagccg cccagctggt cgtacaggaa gatgccggcc gccacatacg gccgctccca
     1921 ccatcgtctg gtgagcggga acaagaacgg gagcggcttg accagatgcg gggcaagcgt
     1981 ggtcagcgat agctcgcgct cgtgcagcgc ctcacgcacc aacccgaact ccagctgctc
     2041 gaggtagcgc aggccgccgt ggaacatctt cgagctgcgg ctcgacgtgc ccgacgcgaa
     2101 atcgcgggcc tcgaccagcg cgaccttcag cccgcgggtg gccgcgtcca gcgccgcgcc
     2161 ggcgcccacc acgccgccac cgatcacgac gacgtcgaac tgctcagaac ccagccgttc
     2221 ccacgcgacc tcgcgttcgc gcgggcccag gaacgtctgc ccattgccgg gagcgggggc
     2281 agcctcgatc acacgtactc cttgttactg gtcggtacta ccaggttaac ggcagtcagt
     2341 cgaggtcgtc gtgcgccatg agccggcggg ccgcctcggt gatcgatccc gtcagcgacg
     2401 ggtacaccga cagcgtctgc gccaggtcgg cgacggacgc ctggttcgag accgccagcg
     2461 cgatcggcaa gatcagctcc gaggcgaccg gcgcgaccac cacgccgccg atcaccacgc
     2521 cggtggccgg gcggcagaag atcttgacga agcccttacg caggccggac atcttcgccc
     2581 gcgcgttggt gttcagcggg aggttcagcg tgcgggccgg aacggtgccg tcgtcaatcc
     2641 tggcctgtgg cacaccgacg gcggcgatct ctggccgggt gaacaccgcg gcggcgacgg
     2701 tgcgcagccg gatcggggta acgccctcgc ccagcgcgtg atacatcgcg atgcggccct
     2761 gcatcgcggc caccgacgcc agcggcagca ggccggtgca gtctccggcg gcgtagatcg
     2821 tcggcaccga cgtgcgcgag acccggtcga ccggaatgta gtcgcctttg ccgagttcga
     2881 tgccgacgcg ctcgagcccc aggccggacg tgttcggcac ggacccgacc gtgatcagcg
     2941 catggctgcc ttccacgcag cggccgtcgg tcatggtgac cacaacaccg tcgtcgccac
     3001 gcgtcaccga ctgggcacgg gccttcttga ccagcgtgac gcccctctcg gcgaacacct
     3061 cctcgagcac ggcggccgcg tcggagtcct cgtggggcag gatctggtcg cggctggcca
     3121 ccacggtcac cggtacgccg agctccgtgt acgcgttgac gaactccgcg cccgtcacgc
     3181 cggagccgac gaccacgagg tggtcgggca gatcggtgag gtcgtagagc tggcgccagt
     3241 tcaggatccg ctggccgtca ggcctcgcgt cgggcagctc gcgggggctg gcgcccgtcg
     3301 cgatcagcac gacgtcggcg tcgagtatct gttcggtgcc gtcgggctcg gtgacctcga
     3361 cgcggtgctg cgccatgccc ggcacggggt cggcgagctc gccgcggccg ctgatcaacc
     3421 gcacaccttg gctgcgcagg cgctcagcga tgtctgcgga ctgcgcggcg gcaagcgtct
     3481 tcacgcggtc gtggatctgc ggcagcgaga ttttcgcgtc ctcgatgtcg atgtcgtaac
     3541 cgagctgcgg ggcgcggcgc aggtcggttc gcacgcccgt cgaggcgatg aacgtcttgg
     3601 acggcacaca gtcgaacagc acgcaggccc cgccgatgcc gtcggagtcg atcaccgtca
     3661 cctcggcggg gtgcgccgcc gcgaccaacg ccgcctcgta cccggccggt cctcccccga
     3721 tgatcacgat gcgggtagcc acagcctcaa cggtagtcac gcgctcagtt gagcgcccac
     3781 atctaacgca accctttagg ctttcccccg tgccgcttta cgccgcgtac gggtcgaaca
     3841 tgcatccgga gcagatgctg cagcgcgcac cgcattcccc gatggccggc accgggtggc
     3901 tgcacggctg gcggctgacg ttcggcggcg aggacatcgg ctgggagggc gcgctggcca
     3961 cgatcgtcga ggatccgaac tccaaggtgt tcgtcgtgct ctacgacatg accaaggaag
     4021 acgagaagaa cctcgaccgc tgggaaggct cagagttcgg ggttcacaag aagattcggt
     4081 gccgtgtcga gcgcgtatca tccgacacca caacggatcc cgtgctggcg tggctgtatg
     4141 tgctcgatgc gtgggagggc ggagtgccgt cggctcgcta tctcggcgtg atggctgatg
     4201 cagctgaaat cgccggtgct ccagcggatt acgtgcatgc actgcgaacc cgcccgtcgc
     4261 gcaacatcgg ccccggccac ggcgactgaa ctcctctttc cgcgagcgtg cgtgtctgta
     4321 cggcgacacg ccgcgacatg acgtacaact gcgcacgctc gcacacgacg aacgtgcgtg
     4381 aaatgaccgc atttgtcggc gtgtcggggt acaaacacgc gcgctcgcgc taaaggaact
     4441 gagccgcttg ctcgatcgtg ttgaccaaca cccgcaggcc gatcgccagc gcgcgttcgt
     4501 cgaggtcgaa tgtcggctgg tgcaagtcca attgaggtcc gcgacccggc cacaccccga
     4561 gccgggccat cgcgccgggc acctcctcga ggtaccagct gaagtcctcg ccgccgccgg
     4621 actgccgcgt ttcggccagc gcgtcggggt cgacggcctc gatcgcgtgc gtgagaatcc
     4681 gcgtcgagac ctcctcgttg accaccggcg gcactccgcg ccggtactgc aacgaatgct
     4741 cgatgcccaa cggcgccagc aacgacgcga cggtctcccg gaccagcgac tccagcgtca
     4801 gccaggtgtc gtggctggcc gtgcggacgg tcccggccag cgagccgctc tgcgggatcg
     4861 cgttgggcgc cgcgcccgcg ttgactgcgc cccacaccat cacggtgctg ttgcgcgggt
     4921 cgatgcggcg cgacaacacc ccgggcagcc cggtgatcag cgtgccgatg ccgtacacca
     4981 ggtcggcggt cagatgcggc cgcgctgtgt gcccgcccgg tgagtgcaga ccgatctcga
     5041 tcgagtcgga cgccgacgtg atcggcccgg cagtgatcgc cacccggccg acgtcgagcc
     5101 gcggatcgca gtgcaacgcg aagatccgcg acacgcccgc cagcaccccg gccgcaatcg
     5161 cgtcgatcgc accgcccggc atcagctcct cggcgggctg gaagatcaac cgcactccca
     5221 ccgggaggtc cggcaccgtc gccaacgcca gcgcggtgcc gagcagcatc gcggtgtgcg
     5281 cgtcgtggcc gcaggcgtgc gcgacaccgg gcaccaccga cgcatacggc gccccggtgc
     5341 gctccggcat cggcaatgcg tccatgtccg cccgcaacgc gatcttcggc aggtgatcgg
     5401 gacccaggtc gcacgtcagc ccgggtccgg cgggcagcac cttcgggttc agcccggctt
     5461 cggccagcag cgttgcgacg aactgcgtcg tggcgaattc ctgacggccc agctcgggat
     5521 gagcgtgaat gtgccggcgc caggcgacca ggtcgtcgta gtgagccgcc agccacgatt
     5581 cggcggtgtc cgcgaggctc atcggcttat cacccggtcc cgctccgaag gcgtctcggc
     5641 gagacgaatc accgtgcgcg ccaacatgat cgccccgtcg atcaccgcgt cgtcaccgct
     5701 gggcgacgcc gcaaccgccg tgaactccgg ctggtgcagc gacgcaccgc ccgtgtccac
     5761 gccgacgacc ggatggatgc cgggcatcgc atgcgtgacg ttgcccatgt cggtactgcc
     5821 cagcggcagc gtggtttcga gttctgcggg catggggctg cggccgcgtc ggatcatctc
     5881 gtcgcggaac acgttcgcca gccacggatc tggtcgcagt tcgtcgtagc cgggcccgct
     5941 gggttcgatt cggcattcac cgccggtggc cagcgcgccg gcgtggaagc attcgaaaac
     6001 ccgcttttcg aggcttttca gcgactcctt gtcggtggcc cggatcgagt accgcatcgc
     6061 cgcgtgtccc gggatgacgt tcggcgcccg gccgccctcg gtgacgatgc cgtgcacctg
     6121 ttgcccgggc gcaaggtgct gacgcagcag accgatggcc acttgcgcga cggtcgcggc
     6181 gtcggcggcg ttgacaccga gatgcggcgc caccgatgcg tgggactcgt ggccgcggta
     6241 gtggacgttt acctctgaca gtgccagcga ccgggccgcg gcgatgtcgg ccggaccggg
     6301 gtgcaccatc accgccgcgc agatatcgtc gaatgctccg cccttgagca gcaatgcttt
     6361 tccgccaccg gactcttccg cgggcgtccc catcagcacg actgtcagcc cgacgtcgtc
     6421 ggccacctcc gcgagcgcca gagcggcccc gaccgcggac gcggcaatga tgttgtgccc
     6481 gcacgcatgt ccgatgcccg gcagcgcgtc gtattcggcg catactgcga cggtcagctc
     6541 gccgctccca tagtcggcgc ggaatgccgt gtccagcccg gcgggggcgg tggtgatcgc
     6601 gaagccccgt tcggcgacca atacctgggc cttggcgcag ctgcggtgtt cggtaaacgc
     6661 gagttccggc tcggcgtgga tgtcatgtga cagcgcgacg aggtcgttac gtctgctgag
     6721 gacgacgtct cgtgccacgt ccgcacgcgt caccgcctca gcatggcggg tgggcggtga
     6781 cgtggcgggc atcatcgcag tatcgcactg aaggctaagc tcgctcagca tgactgcctc
     6841 ggacgacgcc gaacgggcag ccgcggcgat cgccgaacac accg
//
LOCUS       DASWBF010000272         3204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_229412,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000272 DASWBF010000000
VERSION     DASWBF010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229412"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="VF874_10005"
     CDS             complement(<1..150)
                     /locus_tag="VF874_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEX9176392.1"
                     /translation="MAVGSRAKGKFRFRLKAGDGEIIAVGEAYESKASAKKGIASVQT
                     NAADRE"
     gene            complement(369..629)
                     /locus_tag="VF874_10010"
     CDS             complement(369..629)
                     /locus_tag="VF874_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176393.1"
                     /translation="MAHAGSVGLRLQADQTGLTGEVCKAPARRGFVPRHDRGRVLVDV
                     AVMLAGGGEAIADIRCRAILFAGVCPHTCSVLVHVLTSKVGG"
     gene            1041..2198
                     /locus_tag="VF874_10015"
     CDS             1041..2198
                     /locus_tag="VF874_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9176394.1"
                     /translation="MPGATTNADAADPLRIVLVAPPYFDIPPKGYGGTEAVVADLADA
                     LVKRGHDVTVLGAGEPRTSARLIPLWERTVADRLGEPYPEIMHALKVRRVIERIATTD
                     GVDIVHDHTFAGPLNTPAYSAVGIPTVVTVHGPIDDDLYPYYRELGDEVGLIAISDRQ
                     RELAPDLNWVGRVHNALRIDEWPFRADKGDYALFLGRYAPYKGAHLALRAAHEAGIPL
                     VLAGKCNEPPEQAYFNEQVRPLLTENDHVYGQADAVSKRKLLAGARCLLFPIQWEEPF
                     GMVMIEAMACGTPVVALRGGAVPEVIVDGVTGVVCDRPDELPAAIDRAQIMDPVVCRR
                     HVAAHFGVAQFGSGYERIYRTVVGTAVSASRQAKLRRVLRERSNVAEPATA"
     gene            2195..>3204
                     /locus_tag="VF874_10020"
     CDS             2195..>3204
                     /locus_tag="VF874_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX9176395.1"
                     /translation="MTAPPTAFNSGDAVNIGFGGDTVTLVEGATFCLSDGRGDVLAGR
                     SHGLFVRDARVLSRWELRVDGQPAEPLSVQAPEPFVAKFILRRSPRAGLADSTLLLVR
                     ERLIADGLRETISLHNLDDESTVVALMLHADADFADLFSVKEGRAPLGGADMTVRDDE
                     LVLTDRTDRIRGVTVTASGDPIVLPGSFNWRIVVPPRQSWQAEIVVQPTWANQNVTTR
                     FRSGEHQESSGPAHKIEAWRDTATKLEAEHPALTQILRRSESDLGALLIQDDTEDGPP
                     FVAAGAPWFMTLFGRDSLLTAWMALPLDVGVSVGTLQQLAEAQGRRVDPITEEQPGRI
                     LH"
BASE COUNT          552 a         1079 c         1056 g          517 t
ORIGIN      
        1 ttcgcggtct gcggcgttcg tctgaacaga cgcgataccc ttcttcgcgc tggccttcga
       61 ctcgtacgct tcaccgacag cgatgatctc gccgtcgccg gccttaagcc ggaaccggaa
      121 cttgcccttc gcgcggctgc caaccgcgat gcgtcggcgt ggcgcgaacc cgaccgcttc
      181 gaccctgacc gcttcaccca ccagacactc cgagactgct gagcttcggc gccggccccc
      241 ggcggttggc cgagaacccg gcggacattc ccatggcgcg tggtcctcgg ccgcagcccg
      301 actcgactac tcataaattc gggagcggcc gcctgatgag cagcggcggt ctggccgccc
      361 tcatccggtc aaccgcccac cttcgatgtc aagacatgca ctaaaacgct gcaagtgtgc
      421 ggacagacac ctgcgaacag gatcgcgcgg catcggatgt cggcgatcgc ctcaccacca
      481 ccggccagca tcaccgccac atccaccagc acccggccgc ggtcgtgtcg cggaacaaat
      541 ccccgacgcg ccggcgcctt gcagacctca cccgtcaacc cggtctgatc ggcctgcaga
      601 cgcagaccaa cactgccggc gtgggccacc acgccggccc cgtcaccggt taccgacaag
      661 ccagccgacc acgaagtacg cttcacctac gggtgcctgc cagccgggac ttctcggaac
      721 gtcgcaagtc caagaatccc atgcaggaca ggcacttccg tgtttctaca cgctgacagc
      781 ggccatcagc cgtgaacaat ccgggctaac agtccggcat caagtcctcc cgcagccaac
      841 tcagcgcttt ctgcagcagc cgagacacat gcataaccgc agaacaaata gttcccgcct
      901 gacgtgtgag aacagcgcga tggggtatgc gcggtaatga ctttgtagcc gagcgagagg
      961 ggcccttact ggccatgacc gcactgcccc cggatcctga tccagcaaag acccccgacc
     1021 tcttgcccgg tggaggtgtc gtgccagggg caacaaccaa cgccgacgcc gccgatccgc
     1081 tgcggatcgt gctcgtcgcg ccaccctatt tcgacattcc acccaagggc tacgggggca
     1141 ccgaggccgt ggtcgccgac ctggccgacg cgctcgtgaa gcgcgggcac gacgtcaccg
     1201 tgctaggggc cggtgaaccg cgcacgtcgg cacggttaat cccgttgtgg gagcgcactg
     1261 ttgcagaccg gcttggcgag ccgtatccgg agatcatgca cgcgctgaag gtgcgtcgcg
     1321 tcatcgagcg gatcgcgaca acagacggcg tcgacatcgt ccacgaccat acgttcgccg
     1381 gcccgctcaa cacacccgcc tacagcgccg tcgggatccc cacggtggtc accgtgcacg
     1441 gtccgatcga cgacgatctg tacccgtact accgcgaact tggcgacgag gtcggcctga
     1501 tcgccattag cgaccggcaa cgcgagttgg cgccggacct gaattgggtt ggacgcgttc
     1561 ataatgcgct gcgcatcgac gagtggccat tccgcgccga caaaggcgat tatgcgctgt
     1621 tccttggccg gtacgccccg tataaaggcg cgcatctggc cctgcgcgcc gcacacgaag
     1681 cgggcatccc gctggtgctc gcgggcaaat gcaatgagcc cccggagcag gcctatttca
     1741 atgagcaggt gcgcccgctg ctcaccgaga atgatcatgt atatggacaa gccgacgcgg
     1801 tgagcaagcg caaattgctg gccggcgccc gctgcctact gttcccgatt cagtgggagg
     1861 agccgtttgg gatggtgatg atcgaggcga tggcgtgtgg cacaccggtg gtggcgctcc
     1921 gcggcggcgc ggtaccggag gtgatcgtcg acggtgtgac cggcgtggtg tgcgaccggc
     1981 ccgacgagct acccgcggca atcgaccggg cacagatcat ggacccggtg gtgtgccggc
     2041 ggcacgtggc cgcgcacttc ggggtcgccc aattcggttc ggggtacgag cggatctatc
     2101 ggacggtggt cggcaccgcc gtgtctgcca gccgacaggc caagttgcgg cgggttctgc
     2161 gcgagcggtc gaacgtcgcg gagccggcaa ccgcatgacc gcaccgccga ccgcgttcaa
     2221 cagcggcgac gccgtgaaca ttgggttcgg cggcgacacc gtgacgctgg tagaaggcgc
     2281 cacgttttgc ctctccgacg ggcgtggcga cgtgctggcc ggacgctcgc acggtctgtt
     2341 cgtccgcgac gcccgggtgc tgtcgaggtg ggagctgcgg gtcgacggcc agccggccga
     2401 gccgctgtcg gtgcaggccc cggaaccgtt tgtggcgaaa ttcattctgc gtcgttcccc
     2461 gcgcgccgga ttggccgaca gcacgctgct gttggtccgg gagcggctca tcgccgacgg
     2521 gttgcgggaa accatctcgc tgcacaatct tgacgacgag agcaccgtgg tggcgctgat
     2581 gctgcacgcc gacgccgact tcgcggatct gttctcggtc aaggaaggtc gggcgccgct
     2641 gggcggtgcg gacatgacgg tgcgggatga cgagctcgtt ctcaccgacc gcaccgatcg
     2701 cattcggggc gtgacggtga cggcgtccgg cgatccgatc gtgttgccgg gttcgttcaa
     2761 ctggcggatc gtggtcccgc cgcggcagtc ctggcaggcc gagattgtag tccagccgac
     2821 gtgggcgaat cagaacgtga cgacccggtt ccggagcggc gagcaccagg agtcgagcgg
     2881 cccggctcac aagatcgagg cgtggcggga caccgcgacc aagctggaag ccgagcatcc
     2941 cgcgttgacg cagatcttgc gccgcagcga gagcgacctg ggcgcgttgc tgatccagga
     3001 cgacaccgaa gatggcccgc ccttcgtggc ggccggtgcg ccgtggttca tgacgttgtt
     3061 cggccgcgac agtctgttga cggcgtggat ggcgttgccg ctggacgtcg gcgtgtccgt
     3121 gggcaccctg cagcagctgg ccgaggcgca gggccgccgc gtcgacccca tcaccgaaga
     3181 gcagcccggc cggatcctgc acga
//
LOCUS       DASWBF010000273         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_90037, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000273 DASWBF010000000
VERSION     DASWBF010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90037"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VF874_10025"
     CDS             <1..823
                     /locus_tag="VF874_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9176396.1"
                     /translation="TDKRIVITGASSGIGEAAAALFAQQGATVVVVARRQDLLDAVAQ
                     RITTDGGSAIALPCDLSDLDAVDEAVKTVEDRLGGVDILVNNAARSIRRPLAESLDRW
                     HDVERTMALNYYAPLRLVRGFAPGMLQRGDGHIINVATWGVFSEASPLFAVYNASKAA
                     LSSVSRVIETEWSGRGVQSTTLYYPLVATPMIAPTKEYQGLPALTPNEAAEWMITAAR
                     HRPVRIAPRISLFSQALDLVSPDLVTTLMKRSAFNPIPSTPRDRHTAWRSWPAGC"
     gene            796..1182
                     /locus_tag="VF874_10030"
     CDS             796..1182
                     /locus_tag="VF874_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9176397.1"
                     /translation="MEILASRMLIRPADYERSLAFYRDEIGLAIWREYGAGTVFFAGQ
                     SLIELAGHGRDDDVTGTFPGGIWLQVRDIYDTQRELSERGVPIAREARQEPWGLHEMH
                     VVDPDGVTLIFVQVPPEHPLRRDTRR"
BASE COUNT          268 a          543 c          524 g          262 t
ORIGIN      
        1 gaccgacaag cgcatcgtga tcaccggcgc gtcgtcgggg atcggcgaag ccgccgctgc
       61 cctgttcgcg caacagggcg ccaccgtcgt cgtcgtcgca cgcaggcaag acctgctcga
      121 cgcggtggcc cagcggatca ccaccgacgg cggctccgcg atcgcactgc cgtgcgacct
      181 gtccgatctc gacgcggtcg acgaggcggt gaagacggtg gaggaccgcc tcggcggcgt
      241 cgacatcctc gtcaacaacg ccgcccgttc gatccgccgg ccgctggccg agtcgctcga
      301 ccgctggcat gacgtcgaac ggacgatggc gctgaactat tacgcgccgc tgcggctcgt
      361 ccggggcttc gcgcccggca tgctgcagcg cggcgacggg cacatcatca acgtcgcgac
      421 gtggggcgtg ttcagcgaag cctcaccgct gttcgcggtc tacaacgcgt cgaaggcggc
      481 gctgtcgtcg gtgagccggg tgatcgaaac cgagtggtcg ggccgcggtg tgcaatcgac
      541 cacgctgtac tacccgctcg tcgcgacacc gatgatcgct cccaccaagg agtaccaggg
      601 cttgcccgcg ctgaccccga acgaagccgc ggagtggatg atcaccgcag cccgacaccg
      661 ccccgtgcgg atcgcaccgc gcatatcgct gttcagtcag gcgctcgacc tcgtcagccc
      721 cgacctggtc accacgctga tgaagcgcag cgcattcaac ccaatcccgt caaccccgcg
      781 tgatagacac acagcatgga gatcctggcc agccggatgc tgatccgacc cgccgactac
      841 gagcgctccc tcgcgttcta ccgcgacgag atcgggctgg cgatctggcg tgaatacggc
      901 gcgggcacag tgtttttcgc gggccagtcg ctgatcgagc tggccggcca cgggcgcgac
      961 gacgacgtca ccggcacgtt tcccggcggg atctggctgc aggtccgcga catttacgac
     1021 acccagcgcg agctgtccga gcgcggtgtg ccgatcgccc gcgaggcccg tcaggagccg
     1081 tgggggctgc acgagatgca cgtcgtcgat cccgacggcg tcacgctgat cttcgtgcag
     1141 gtcccgcccg agcatccgct gcgccgcgac acccggcggt gactcagcgc gcgccggcag
     1201 cctcgcatca accgatggcg ttcaccgcgc tcgaaacttt cgatcggagg gcagcggaaa
     1261 gcacccgtga tcctcaagtg ctgctgggct ggaagcacac ctggtcggtg actgcgccga
     1321 ggtgggctag atccgcggcg cactcagaac cgcggccgac gtcgcggctc tggtgtgggc
     1381 actgtcaact tgagcaatcc gagggcgatt ttcgccggtt cgcctccgcg attcaccagg
     1441 tcgcgtgtga cttatgaggc ttatgtggtt agcaaaacgt ccacacgacc tcaagttgac
     1501 agtgccgctg gaagtgttcg gcatcgacgg cgggcgtgac gtcgactttt ctcagcagga
     1561 tcttcgacaa gctgctggtg aacttgacct ggtgggt
//
LOCUS       DASWBF010000274         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_229621,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000274 DASWBF010000000
VERSION     DASWBF010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229621"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VF874_10035"
     CDS             <1..1615
                     /locus_tag="VF874_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEX9176398.1"
                     /translation="RDALRSGDVHVPGSRRYADPTAYLIAIDAWPAQREEFCRLVGVA
                     AASEDALAQVETELGAALGDLEGVLADGQGPVRLDEDGHLVIGPLTAEDVPGEVEQLK
                     DELTALIPFAPIASVLVELDRQTGFLDFFTHAGGAKPRSRELKRNLIAVLIANATNMG
                     LSRMSDASGIAYDTLAWTQEWYVREDTLRAANLSLVAYHQQLPLTAMFGSGTLSSSDG
                     QRFPTKGSSVTARALSRYFADQGLSTYSHVTDQHAVFGTKVIVATEREAPYVLDEILG
                     NQTDLPITEHATDTHGASLINFALFDLVGLQLSPRIRDLGKITLHRFGSHAETADAYP
                     HAGLLLSRRINLGLIAENWDEMLRLAASLKYGHVTASLIVAKLSRADRQNSLAAALKE
                     YGALRRTVYAARYLSREDYRRKISRQLNKGESVHALKRDVFYAHEGAVRRCHLQQQSE
                     QAWCLTLTTNAITTWTTEYLGLAVDHLHGRGRRVDPQWLAHISPAQSDNIGLFGTITV
                     DIDAELARLDPAGHRPLRNPPTVDSAPSV"
     gene            1802..2104
                     /locus_tag="VF874_10040"
                     /pseudo
     CDS             1802..2104
                     /locus_tag="VF874_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117340.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2236..2730
                     /locus_tag="VF874_10045"
     CDS             2236..2730
                     /locus_tag="VF874_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176399.1"
                     /translation="MDRKPRLEQIDADPESLGAIFAEQMLTPEQEGQPRTWWYDPQNP
                     RGCWITETLDGTYHATVGTTVPATGRLQEFELESGLAAFFRDDAGMVWPIPPRGLDYF
                     NSGYAGTGPQELVEAVTALRINAAADIDTAAHMTADAALSELIMTHTPPLNVSQHQIN
                     ALLP"
     gene            2807..2989
                     /locus_tag="VF874_10050"
     CDS             2807..2989
                     /locus_tag="VF874_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176400.1"
                     /translation="MRSRYLVMRYWRRSRSWRAWCARGGVGGGGQVGRGALGGGVAVA
                     AGGWGAQVLGPGQIGG"
BASE COUNT          509 a         1006 c         1002 g          498 t
ORIGIN      
        1 gcgcgacgcg ttgcgcagcg gcgatgtcca cgtgcccggt tcccgccgct acgccgaccc
       61 gaccgcctat ctgatcgcca tcgatgcgtg gcccgctcag cgtgaggagt tctgccgcct
      121 cgtcggcgtc gccgccgcgt cggaggacgc tcttgcccag gtcgagacgg agttgggtgc
      181 cgcactcggt gatctggaag gggtgctcgc cgacggacag ggaccggttc ggctcgacga
      241 ggacgggcac ctggtcatcg ggccgttgac tgccgaggac gtccctggtg aggtcgagca
      301 gctcaaggac gagctcaccg cgttgatccc gttcgcgccg atcgcgtcgg tcctggtcga
      361 attagatagg cagacagggt ttttggactt tttcacccat gccggcggcg cgaagccgcg
      421 ctcgcgcgaa ctcaagcgaa acctgatcgc agtcctcatc gccaatgcca cgaacatggg
      481 tctgtcgcgg atgagcgacg cctccggtat cgcctacgac accctggcgt ggacccagga
      541 atggtatgtg cgtgaggaca cgctgcgcgc ggcgaacctg agcctcgtcg catatcacca
      601 gcagctgccg ctgacggcga tgttcggcag cggcacactg tcgtcctcgg acgggcaacg
      661 gttccccacc aagggcagct cggtcaccgc cagagccctt agccgctact tcgccgacca
      721 gggactgtcc acctactccc atgtcaccga tcagcacgcg gtgttcggca ccaaggtcat
      781 cgtcgccacc gagcgggagg cgccgtacgt gctcgacgag atcctcggca accagaccga
      841 tctgccgatc accgagcacg ccaccgatac ccacggggcg agtctgatca acttcgcgtt
      901 gttcgacctg gtcgggttgc agctgtcgcc gcgcatccgc gacctcggca agatcacgct
      961 gcaccgattc ggttcgcacg ctgagacggc cgatgcctat ccacacgccg gtctgctgct
     1021 gtcgcggcgg atcaatctgg ggttgatcgc cgagaactgg gacgagatgc tgcggttggc
     1081 cgcgtcgctg aaatacgggc acgtgaccgc ctcgctgatc gtggcgaaac tgtcccgcgc
     1141 cgaccggcag aacagcctgg ccgcggcgtt aaaggagtac ggggcgctgc ggcgcacggt
     1201 ctacgccgct cgttacttgt cgcgggaaga ctaccgccgc aagatatctc gccaattgaa
     1261 caagggcgag agcgtgcacg cgctcaaacg cgacgtgttc tacgcccacg aaggcgcggt
     1321 gcgccgctgt cacctgcagc agcaatccga acaggcgtgg tgtctgacgc tgacgaccaa
     1381 cgccatcacc acctggacga ccgaatatct cggcctggcg gtcgatcacc tgcacggtcg
     1441 gggtcggcgt gtcgaccccc agtggctcgc gcacatctcc ccggcgcaat cggacaatat
     1501 cggcctgttc ggcaccatca ccgtcgacat cgacgccgaa ctcgcccgac tcgaccccgc
     1561 cggacaccga cccctgcgca acccgccgac ggtggactcg gcgccgtccg tttgacgaac
     1621 ggcggattct ggcagtcgtc gcaacacgtc ccgtcgcgag cggaatcgct cgtccgtgtt
     1681 gagcgtagcg gggtcgggtc ggtggcgggc gctggtacgg gccggcatgc ccacgcgtca
     1741 agggcgctgc gcgtccctgc gggaccctcc ggccctgggg cagggagttg cgggcagggc
     1801 gatggccctg cccctcaccg gcaccaaccg tagacgcccc ggtctcgacc aggccctcgc
     1861 cgcggtacgc gagggcgaca ccctggtcgt gccgaaactc gacaggctcg cccgctccgt
     1921 gcccgacgca agagcgatcg gcgacgacct cgccgcgcgc gggatcaggc tctccctcgg
     1981 cggccaggtc tacgacccca ccgacccgat gggcaagatg ttcttcaaca tcctcgccac
     2041 cttcgccgaa ttcgaagtcg acctgctcag gctgcgaact cgcgccgaac tgttcaaagt
     2101 ctccagaccg acggtaggtc tgtgatggtg tagtcgccgg tgccgtgcat ccggcgttcg
     2161 ctagcgcgct cgtcacggcc cgcgcgcgat cacagacccc atgtcatgcg gcatctggcg
     2221 ttgtttttgc accgaatgga ccggaaaccc cgactggaac agatcgacgc cgatcccgaa
     2281 tcgctcggtg cgatcttcgc cgagcagatg ctgacccccg aacaggaagg ccagccgaga
     2341 acgtggtggt atgaccccca gaatccacgc ggatgttgga tcaccgaaac actcgacggc
     2401 acatatcacg ccaccgtcgg aacaaccgtg ccggccaccg ggcggctcca agagttcgag
     2461 ctggaaagtg gcctagcagc attctttcgc gacgatgccg gcatggtgtg gccgataccc
     2521 ccgcgcgggc tggactactt caacagcggc tatgccggaa caggacccca ggaactggtc
     2581 gaagcggtga cagcgctgcg catcaacgcc gcggccgaca tcgacacggc agcgcacatg
     2641 accgcggacg cggcactgtc agaactgatc atgactcaca cgccaccact caacgtttcg
     2701 cagcaccaga tcaacgccct cctaccgtga cgggactggc gcggcttaca gttcgagtcc
     2761 gtcgcggtgg cggtttcggt tgtggccgtc gatggtgcgc tcgcgggtga ggtcgcggta
     2821 tctggtgatg aggtattggc ggcggtcgag gagctggcgg gcgtggtgtg ctcgcggcgg
     2881 tgtcggtggt ggtggccagg taggccgcgg cgcgctgggc ggcggtgtgg cggtggcggc
     2941 cgggggttgg ggtgcccagg tgttgggccc agggcagatc ggcggctagt gcgtcggtga
     3001 gggtgtgggc gggtg
//
LOCUS       DASWBF010000275         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1139394,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000275 DASWBF010000000
VERSION     DASWBF010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139394"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..664
                     /locus_tag="VF874_10055"
     CDS             56..664
                     /locus_tag="VF874_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9176401.1"
                     /translation="MTAQLPLAPEDARPVGAAAAIVEDADGGRVFVHGNLVYAWDAGD
                     AAGRRFAAVSLMRIKAATQLLQAARTAGDPFVRLCVEFLARTGLRKGEFLDLTVDSVV
                     QIGAAYWLHVPLGKLRTDRYIPLHPQLKDLLDAWLAARPTSLRSRYLFVEHGQRIGEG
                     RVDRAVAQTARAAGIGHVSLDPARPDGGARQAIRQSARLVRP"
     gene            complement(1112..1999)
                     /locus_tag="VF874_10060"
     CDS             complement(1112..1999)
                     /locus_tag="VF874_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176402.1"
                     /translation="MTKTDRAMFSATTALYPDEMVERANALGPMLAKRSKARAHYTAG
                     ARQRSRRTRTEVFDLNDALQYGRFRFTHYLDSPEEVARGGGSSVRDRYSAESIFVPRT
                     PDNEQRVGELVAQYNQSRRQHATIQYATALTRDGGEPREVIYVKGPRKRVTTEVTGVS
                     AEVTFSDSSSMVHELGHRMEDRNPEISAATKAFLRRRTAGLPFQRYHKRELVVEDSFA
                     DKYMGKDYPGEYHTELFSCGMEALTHGRFGGLRGQSTVFLPAPGGISAADRAQPPRAD
                     SEHLALMLGLLCSANRRIP"
     gene            complement(1996..2688)
                     /locus_tag="VF874_10065"
     CDS             complement(1996..2688)
                     /locus_tag="VF874_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176403.1"
                     /translation="MCRAVVDGGRRCPCTRGDRRRAYQRMRYAARQAAAAAPPASDEH
                     TVAATADPDTTALSALEQRRTVTAATVNEALTVLRDPDRSCDPDVHEAYLDAVLEHGA
                     VVRDIADHKIDQAYAERGLNDASVNAEAAAIAPELERIESEWREAKTRTDDYLTAAGD
                     RSSATRPPPRSMPPATPTSPPNRTSTARPPSGATRSTSNGARSRGTCTTRNWPGNAAS
                     ALPRSSRPTPPR"
     gene            complement(2717..3040)
                     /locus_tag="VF874_10070"
     CDS             complement(2717..3040)
                     /locus_tag="VF874_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176404.1"
                     /translation="MIAYSALERPQPDEPGGIAGGLLGGDPSLVAIVREILAGSLPKK
                     VRVAEPNLDYVFTPTRDTLADVAAAMLVAGNGRGRLSDSGWDALDAAMADEDADTDTD
                     GPIIH"
     gene            complement(3173..4162)
                     /locus_tag="VF874_10075"
     CDS             complement(3173..4162)
                     /locus_tag="VF874_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176405.1"
                     /translation="MWTEIARAAHVIEPFAKSGALAAAGLAAVMMLLHWRPHPGVSVV
                     EMLSASRPFGYIAIGLGAAAVLFRFARRRAKVHPHWLYTADELTTLDAACIDWPAVPE
                     ELDRYANGETLRREWFSRWLWRMQPGAAALVWREPHLVAVATLLSRDIRSSAAWNSDL
                     FDIHRVRIDLEQTLDGIYLRAHRIWRARANLVPPPTRHRDDMVARRNTEITDAACHAW
                     DTLVELVRQLQDYKRQLAPIDAVFSEITALQQSSLRITDDAVRQLHVDAAGNTLHAGA
                     VGAATIELAELNANLAARLRTLRHSLTAATNGLVLQPAMTYQLTIGRLTPNPA"
     gene            complement(4072..4479)
                     /locus_tag="VF874_10080"
     CDS             complement(4072..4479)
                     /locus_tag="VF874_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176406.1"
                     /translation="MQICDEGIEVGTEQFDEPESDTNWTQTDGYAQDSRVPLHPPIDA
                     GVRAPLDYDYSSDTMAALPRSDAIDAGALPADLQRKARAHGDDLDNIVESEQLTTRPP
                     AATSCGLRSPALHTSSSRSPSPAHWQPRAWQPS"
BASE COUNT          667 a         1419 c         1653 g          904 t
ORIGIN      
        1 caataataga tatagtgatt tcgtgtgatt cggggagtgg tggacgatgg ccgtgatgac
       61 cgcgcagctg ccgctggcgc ccgaggacgc gcgcccggtc ggtgcggccg cggcgatcgt
      121 cgaggacgcc gatggcggcc gggtgttcgt gcacggcaac ctggtctacg cgtgggatgc
      181 cggcgacgcc gcggggcgcc gcttcgcggc ggtgtcgctg atgcggatta aggcggccac
      241 ccagctgctg caggccgcgc gcaccgccgg cgacccgttc gtccgactgt gcgtggagtt
      301 cctggcccgc accggcctgc gcaaaggcga attcctcgat ctcacagtcg attccgtggt
      361 gcagatcggc gccgcgtact ggttgcatgt gccgctgggg aagctgcgca ccgaccgcta
      421 catcccgttg catccccaac tgaaggatct cctcgacgcc tggctcgccg cccgacccac
      481 cagcctgcgc agccgctact tgttcgtcga gcacggccaa cgcatcggcg agggccgcgt
      541 ggatcgggcc gtcgcccaaa ccgcccgcgc cgccgggatc ggccacgtca gcctagatcc
      601 agcacggccg gatggcggcg cgcggcaagc aattcggcaa tcggcgcgcc ttgtccgacc
      661 gtaaggtcga cgtcagcgtg ctcgccgccg aaaatcacca ccggtaaccc ggcggatcgc
      721 ccatctgctg aggatctcag tccgtcccac acaccggtgg gatcaatcac gcaggtgacg
      781 cagccgcagc gaatcagtcc ttcggtcagc accgctgcgg tgaatgtctt gccccgccct
      841 ttctttgccc aggattccga acgtcgctgt aacactgtct agaggcaaag tgaagtcccg
      901 gccagctcaa taccagcctg actgtctgga tgcattcggc gttgggtggt catcgcattt
      961 cctgagctaa acggcttgac gccacctcga gtatccagag tcgaagcgtc cggcggcggc
     1021 ctgacaccag tatgagcggc cttcatttcg cctcaggccc cccggctgct gcgacgacag
     1081 gggcgcgcgg cggcgcggcg gcatcagggg ctcacgggat gcgtctgttg gctgagcaaa
     1141 gcagtccgag catgagcgcc agatgctcgg agtcggcgcg gggcggttgg gcacggtcgg
     1201 cggcgctgat tccgccgggt gctggcaaga agaccgtcga ctgaccgcga aggcctccga
     1261 agcggccgtg tgtgagcgct tccattccgc agctgaatag ctcggtatgg tattcgcctg
     1321 ggtagtcctt gcccatgtac ttgtcagcga aactgtcctc aacaaccagc tcacgtttgt
     1381 gataccgctg gaatggcagg ccggcggtgc gccgacgcag gaatgccttt gttgccgcgg
     1441 aaatttcggg gttgcggtcc tccatgcggt ggccaagctc gtggaccatc gacgatgagt
     1501 cactgaaggt cacttcggcg ctgacacccg ttacttccgt ggtgacgcgt ttccgtggcc
     1561 ccttgacgta gatgacttcg cggggctctc ccccatcgcg tgtcagcgcg gtcgcgtatt
     1621 gaatcgtcgc gtgttggcgg cggctttggt tgtactgcgc caccaattcg ccgacccgct
     1681 gctcgttatc gggtgtgcgc gggacgaaga tcgattctgc gctgtagcgg tcgcgcaccg
     1741 atgagccgcc gccgcgggct acctcttcgg gtgagtcgag gtagtgggtg aaccggaagc
     1801 ggccgtactg cagggcgtcg ttgaggtcga acacttcggt tcgggtgcgc cggctgcgtt
     1861 gtcgggcgcc ggcggtgtag tgcgccctgg ccttggagcg tttggcgagc atggggccca
     1921 gagcgtttgc acgctcgacc atttcgtcgg ggtatagcgc ggtggtggca ctgaacatgg
     1981 cgcggtcggt tttagtcatc ttggcggtgt tggacgggat gaacgtggca gtgccgaagc
     2041 tgcgttccct ggccagttcc tcgtagtaca cgttccgcgc gatcgcgcac cgttgcttgt
     2101 tgatctcgtt gctccgctcg gtggcctggc ggtagatgtc ctgtttggcg gcgatgtagg
     2161 tgtcgcgggc ggcatcgatc gcggtggcgg cctcgtcgca gacgaacgat ctcccgcggc
     2221 ggtgaggtag tcgtcggtgc gtgtcttggc ttcacgccat tcggattcga tccgttcaag
     2281 ttccggcgcg atggcggccg cctctgcgtt gaccgacgcg tcgttaagtc cgcgctcggc
     2341 ataggcctgg tcgatcttgt ggtcggcgat gtcccgcacg accgcgccgt gctctaggac
     2401 ggcatctagg tacgcctcat gaacgtcggg gtcgcagctg cggtcggggt cgcgcagcac
     2461 tgtcagcgcc tcgttgacgg tggccgcggt gacggtgcgg cgttgttcga gggccgacag
     2521 tgccgtggtg tcgggatcgg cggtcgcggc gactgtgtgc tcgtcgctgg ccggcggcgc
     2581 cgcggccgcg gcctgtcggg cggcgtagcg catccgttgg taggcccgcc gccggtcgcc
     2641 acgcgtgcag gggcagcgcc ggccgccgtc gactactgct cggcacatgc cagtgggtgt
     2701 cgtggctgcg gcatggtcag tggatgatcg ggccgtcggt gtcggtgtcg gcatcctcgt
     2761 ccgccatggc tgcgtctagg gcgtcccagc cggagtcgga gagccgcccc ctgccgttgc
     2821 cggcgacgag catggcggcc gccacgtcgg caagggtgtc gcgggtgggg gtgaacacgt
     2881 agtcgaggtt gggttcagcg acgcgtacct tcttgggcag gctgccggcg agaatctcgc
     2941 ggacgatcgc gacaagcgag gggtcaccgc cgagcaggcc gccggcgatt ccgccgggtt
     3001 catcgggttg cggtcgctcc agtgcgctgt aggcgatcac accgcggtcg gtgctgaatg
     3061 tgatgtggtg ggagccattt cggtttctgt gtcttcctgt tccgcggtta ctggtcgatc
     3121 tcgatgaaga tgttgcgggc agcgatcggg tggcctgcgc ttcggcggct ttctaggccg
     3181 ggttgggtgt gaggcgaccg atggtcagct gataagtcat ggcgggctga aggacaagac
     3241 cgttggttgc agcggtgagg ctgtggcgca gggtcctcag acgcgccgcg aggttggcgt
     3301 tgagttcggc caattcgatg gtggccgctc cgaccgcgcc ggcgtgcagg gtgttgccgg
     3361 cggcgtcgac gtgcagctgt cgcacggcgt catcggtgat gcgcaggctg ctttgttgca
     3421 gcgcagtgat ttcggagaac accgcgtcga tcggcgcgag ttgcctcttg tagtcctgca
     3481 actggcgcac cagctcgacc agggtgtccc atgcgtggca ggcggcgtcg gtgatttccg
     3541 tgttgcgtct ggccaccatg tcgtctcggt gcctggtggg cggcgggacg aggttggcgc
     3601 gggcgcgcca aatacgatgc gcccgtaaat aaattccgtc aagggtctgt tcgaggtcaa
     3661 tccgcacccg gtggatgtcg aacagatcgc tgttccatgc ggcgctggag cggatgtcac
     3721 ggctaagcag ggtggccaca gcgaccagat gcggctcacg ccataccaga gccgccgccc
     3781 caggttgcat gcgccaaagc catcggctga accattcgcg gcgcagagtc tccccgtttg
     3841 cgtaacggtc gagttcttcg gggaccgcgg gccagtcgat gcacgcagcg tcgagggtcg
     3901 tgagctcgtc ggcggtgtac agccagtgtg gatgaacttt ggcacggcgg cgcgcgaatc
     3961 ggaacagaac tgcggcagcg cccagaccaa tggcgatgta gccgaaaggc cttgatgcgc
     4021 tgagcatctc gacgacggac acgccgggat gtggcctcca gtgcagcagc atcatgacgg
     4081 ctgccaagcc cgcggctgcc agtgcgccgg acttggcgaa cggctcgatg acgtgtgcag
     4141 cgcgggcgat ctcagtccac acgaggtcgc cgcgggcggc cgggtggtca actgttcgga
     4201 ttcgacaatg ttgtcgaggt cgtcgccgtg ggcgcgggct ttgcgttgca ggtcggccgg
     4261 cagggcgccg gcgtcgatgg catccgaacg cggcagcgcg gccatggtgt cgctcgagta
     4321 gtcgtagtcc aggggcgcgc gcacgccggc gtcgatcggc gggtgtagcg gcacccgact
     4381 gtcttgagcg tagccatccg tctgcgtcca gttcgtgtct gattccggct cgtcgaactg
     4441 ctcggtgccc acttcaatcc cctcatcgca gatctgcatg tagtcactat cggcgcttcg
     4501 agcgacacgg ccgatgaacg gcggcggcgg ctgggtagcg ctccctggtg acctgcgtct
     4561 gaagggttcg ccgacagccg caggtttcac ttcacctcgg ttcagctcct ctccccgttg
     4621 gcgatgccgc gggccccgcg gcc
//
LOCUS       DASWBF010000276         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1011881,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000276 DASWBF010000000
VERSION     DASWBF010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011881"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..964
                     /locus_tag="VF874_10085"
                     /pseudo
     CDS             1..964
                     /locus_tag="VF874_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407620.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            961..1686
                     /locus_tag="VF874_10090"
     CDS             961..1686
                     /locus_tag="VF874_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9176407.1"
                     /translation="MTTPPATPQRRTPPKRKARQLAKHLRDERPDYAYLKAVFRALRD
                     ELDVDVQRQPKTLPYVPSEDEIRRYYQTVWQARRSGDMILIKTLLYTGVRVAELVAIG
                     IDDVHLDACRIRITHGKGGKDRVVPFPQTFRETLALHIADRHKTGGTYLFESSWKKPY
                     STRGVRALLARYANAAGLPHNMPPHRLRHFLFTWLKTQGIDDALIQPYSGHASRQSLE
                     IYSRLALSDAQASYDQAIGRFPV"
     gene            complement(1634..>1959)
                     /locus_tag="VF874_10095"
     CDS             complement(1634..>1959)
                     /locus_tag="VF874_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176408.1"
                     /translation="RVRAQRMQRFVNAHQHDPGDRGVYRIALANPNTDRVLADREWGQ
                     VAERFVARLGADKGLWEATRHDRHHIHLTIRPGPPERVASRKLGMIFRRGIGRWLGRS
                     WLGRR"
BASE COUNT          352 a          745 c          576 g          286 t
ORIGIN      
        1 acgcgaacta cgccgcacgc gacgccgaac tgtggggcgc aggcacgacg tgcgcgtcgg
       61 attcgaagaa gttcggctct gggagtcgaa cctgatgacc gaatggcacg cccgctaccg
      121 cggccccggc gtaatgatct actggcacgt cgagaagggc cggctgtgcg tgtacagcca
      181 gctcaaatcc tgctcgtcct ccgaggtcgc ggcgatgatg gaaggcctgc tgcaccacgg
      241 caccgacgtg ccgatccagg ccaactacgt cgacacccac ggcgcctccg aggtcgggtt
      301 cgccttcacc cacctgctcg gctactcgct gctgccgcgc ttgaagaaca tcggcgccgc
      361 gcggctctac ctacccgatg ccggccgcgg cgtgtcggtg ccgcacctgc agacggtgct
      421 gactcggccg atccgctggg agctgatcgc cgagcagtac gaccagatgg tcaagttcgc
      481 caccgctctg aaactgcgca ccgccgaggc cgagcagatc ctgcgccgct tcacccgccc
      541 cggcccgcag caccccacct acgccgccct cgtcgagctc ggccgcgcgg tcaagtccac
      601 ctttgtcgcc cgctacctgc gctcggaagc gctgcgccgc gagatcaacg atgggctgca
      661 ggtggtggag aactggaact ccgccaacgg cgccctgttc tacggcaagg accgtgaact
      721 caccggcgcg gatcgcgaca gccaagaagt cagcgttctg gcgctgcacc tgctgcaatc
      781 cgcgctggtc tacgtcaaca ccctgctgct gcaacgcatc ctggccgacc agcccatcaa
      841 cctcaccgcc gaagaccggc gggcgctgtc gccgctgttc tggacccacg tgcgcccgta
      901 cggcaccttc gagctgcacc tggaccggca cctcgacctc gacccgcacc cggtcagcgc
      961 atgacaaccc cgccggcaac acctcagcga cggaccccgc cgaaacgcaa agcccgccag
     1021 ctggccaaac acctgcgcga cgaacgaccc gattacgcct atctgaaggc cgtcttccgg
     1081 gcgctgcgcg acgaactcga cgtcgacgtg caacgccaac ccaagacgct gccctacgtg
     1141 cccagcgagg acgagatccg ccgctactac cagaccgtct ggcaagcccg gcgcagcggc
     1201 gacatgatcc tgatcaagac actgctctac accggcgtcc gcgtggccga gcttgtcgcc
     1261 atcggcatcg atgacgtcca cctggacgcc tgccggatcc gcatcaccca cggcaagggc
     1321 ggcaaagacc gggtcgtgcc gttcccgcag accttccgcg agaccctggc cctgcacatc
     1381 gccgatcggc acaagaccgg cggcacctac ctgttcgaat cctcctggaa gaagccatat
     1441 tcgacccgag gcgtgcgcgc cctgctcgcc cgctacgcga acgccgccgg gctgccgcac
     1501 aacatgccgc cccaccggct gcggcacttc ctgttcacct ggctcaagac ccaaggcatc
     1561 gacgacgccc tgatccagcc ctacagcggc cacgccagcc gccaatcact cgagatctac
     1621 agcagactcg cgctcagcga cgcccaagcc agctacgacc aagccatcgg ccgattcccc
     1681 gtctgaagat cattcccagt ttgcggctgg cgacacgctc aggaggtccg ggcctgatgg
     1741 tcaggtggat gtggtggcgg tcgtggcggg tggcctccca caagccctta tcggcgccca
     1801 agcgggccac aaaccgctcg gcgacctgac cccattcgcg gtcggcgagc acccggtcgg
     1861 tgttcgggtt ggccaacgcg atccgataca ccccccgatc acccgggtcg tgttggtggg
     1921 cgttgacgaa ccgctgcatg cgctgcgcgc gcacccgcc
//
LOCUS       DASWBF010000277         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_808680,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000277 DASWBF010000000
VERSION     DASWBF010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808680"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1554
                     /locus_tag="VF874_10100"
                     /pseudo
     CDS             1..1554
                     /locus_tag="VF874_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970816.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
     gene            1606..>2433
                     /locus_tag="VF874_10105"
     CDS             1606..>2433
                     /locus_tag="VF874_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX9176409.1"
                     /translation="MSDTAVNPDVDIDLRDRFRPRPDAATLARSRGVPVLDIVVPVYN
                     EQVNLANSVRRLRRYLRQHFPFEARITIADNASTDDTPQIAAQLAEDLTDVRVVRLEQ
                     KGRGRALHDVWSTSDAPVLAYMDVDLSTDLAALAPLVAPLISGHSDLAIGSRLARGSR
                     VVRGAKREVISRCYNLLLKSALAAGFSDAQCGFKAIRSDVARRLLPHVTDTGWFFDTE
                     LLVLAQRSGLRIHEVPVDWVDDPDSRVDIVATAAADLKGITRLMKGFATGAIPVQTLA
                     "
BASE COUNT          345 a          820 c          827 g          441 t
ORIGIN      
        1 cagcgctgtg ggtgatggac gtgtcgatgc ggctgttcgg gttcaactcg tggagtgtgc
       61 ttgcgccgca agcactcgaa ggcgtggcag cggtggcggt gctgtacgcc gcagtgcgac
      121 gtgtcaacgg tgcgacggcc gctctgttgg ccggggcggt gctcgcgctg acccccgtcg
      181 ccgcgctgat gtttcggtac aactaccccg acgcgatgct ggtgttgctg ctcgtggccg
      241 ctgcctactg cgtgcagcgc gcctgcgaaa acgaaagcag ccgttggtgg ttgccgctgg
      301 caggggtcgc ggtcggcctc gcgtttctgg ccaagatggc gcaggcgttc ttggtggtgc
      361 cgggattcgc ggtggcgtat ctgctcagcg cgcatgcgcc gctgcggacg cgtctgacac
      421 gctcggcgtc cgcggccgtc gcgatgctgg tgtccggcgg ttggtatctc gtgttggtga
      481 ctctgtggcc cgcggatcga cgcccgtaca tcggcggatc gcagcacaat agtatgtcga
      541 actcgcactg ggctacaacg gattcggccg actgaccgga gacgagccgg gtgggctggg
      601 taatcccaat cacgatgccg gctgggcgcg gctgttcggc gccggcatgg gcgccgagat
      661 cgcgtggctg attcctgccg cggtgatctg cctcgcggca gcgctggtga tcacccggcg
      721 ggcaccgcgc acggacgcgg tccgaggagt ttacgtcatg tggagcggct ggctggtggt
      781 gaccggcgcg gtgttgtcgt actccagcgg catcatccat ccctactaca cggtcgcgct
      841 ggccccggcc gtcgcggcag gcatcggcat cggcgctgcg acagcgcttt ccggcgcagt
      901 gctggtgacg acggtactgt cctgcgtgtt gctggcacgc actcaaacgt ggctaccgtg
      961 gctgcggccg acgattgcgg tcaccggcgt cggtgcggcg ctctgctcat cgtcgtcggg
     1021 cggtcttccc gggcggcttc ccgcgcggtc gcgggccttg cggttgccgc gtgcttggcg
     1081 ggccccgccg cgtactccgt ggccaccacg atgacaccgc accacggcgc cgtccccagc
     1141 gtgggaactt cgcgcgcaac gggtttcggc cctggcggct tgtttgactc acccgaacgg
     1201 tcgccccagc tcgtggctgc gttaaccgca gacgccgacc agttcacgtg gaccgctgcg
     1261 accgtcggct cgtcgaacgc cgccggctac caactggcca tcggcgaacc ggtgacggcg
     1321 gtcggcggct tcaacggcac cgatcccgca ccgaccctcc accagttccg aatgctggtc
     1381 attgacagaa ggattcacta cttaatcggg gcgacgatgc gggcaccgat gcgccctgcc
     1441 tcggtcggca gcggcgaggc gacagacatc gccgcatggg tggccgccca ttccctggcc
     1501 cgcactatcg acggcgtcac cgtctacgac ctgaccgcgg gaactcacag ctgacgcata
     1561 gcgtcggccc acggtgtacc cacccagggt cgccagcatg gtgccatgag cgacaccgct
     1621 gtgaaccctg acgtcgacat cgatctgcgc gaccgctttc ggccccggcc cgacgccgcc
     1681 acgttggcgc gctcgcgcgg cgtgcccgtc ctcgacatcg tggtgccggt gtacaacgag
     1741 caagtcaacc ttgcgaactc agtgcgtaga ctgcgccgct atctgcgaca acactttccg
     1801 ttcgaagccc gaatcacgat cgcggacaac gccagtaccg acgacacccc gcagatcgcc
     1861 gcgcaactgg ccgaagacct gacggacgtg cgtgtggtgc ggctggaaca gaagggccgc
     1921 ggacgcgctc tgcacgacgt gtggagcacc tcggacgctc cggtgctggc gtacatggat
     1981 gtcgacttgt ccaccgacct cgcggcgctc gcgccgttgg tggctcccct gatttccggc
     2041 cattccgacc tcgcgatcgg ctctcgcctg gcccgaggat ctcgagtggt gcgcggcgcc
     2101 aagcgggaag tgatttcgcg ttgctacaac ctgcttctca agtcggcgtt ggcggccggc
     2161 ttttcggatg cgcaatgcgg gttcaaggcg atccgctccg acgtggctcg gcggctgctc
     2221 ccccacgtca cggacaccgg gtggttcttc gacaccgaac tgctggtgct cgcgcagcgc
     2281 agcggcctgc gcatccacga ggtcccggtg gactgggtcg acgaccccga cagccgtgtc
     2341 gacatcgtcg ccaccgccgc cgccgatctc aaaggtatca cccggctgat gaaaggtttt
     2401 gccaccgggg cgattccggt gcagacgctc gct
//
LOCUS       DASWBF010000278         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_755697,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000278 DASWBF010000000
VERSION     DASWBF010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755697"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2155
                     /locus_tag="VF874_10110"
     CDS             <1..2155
                     /locus_tag="VF874_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9176410.1"
                     /translation="AVVKGAEGPITYHLVLGECADAGRVGVGRGPLIGRDRELARLQR
                     SWARAQAGTLTVPGVVFRGEPGIGKSRLAAAAVELAEDSGAVVLELIGSPFHTDTGLH
                     PVRTLIERRCGIDRMTDPAEQLRMLHAEVAARSLDPVRLVPLLAPVLGIAPAAGYEPV
                     PAEGRKLYELIGEAVREYLLACLGDDAGLLVTEDVQWFDASTLEVLGCLLTAARGRLL
                     VVIAGRPGGWLPGGWRVKVFDLQPLTDEQADRLITALDPSLPAKDRAAVCARCDGVPL
                     YIEQLVRGAREKPAGDSAETRVPDALYEPLLARLHASAEVVPVVEAAGIIGRHVDRGL
                     LCSLVALSEDEVDHIIDELEDALVLEPSGTDGWQFRHELLREVAAELAPPSVRRGLHA
                     KVADALVHRVDGGDPDWGLVARHYEQAQRFDSSAWAYQQASAAARRRGALAEARTYLT
                     LALAGLDRATSGPDRDRREIALRLERGFLTTAVSGYTEVGDFERCLQLWGTELWDDDL
                     VVTLFALAGYYTLRADLRRAAQVLESLRAGAGEGPPWLRPVIDGGFGVVAWLRGEFDA
                     AGSYLEQATRGLASADLHEIDAVWIQPDEPVASVHVYLALVRLVRGDFSGAETELAQA
                     ARRADELGFPQGPYVRAMACYVEIWLRIEGGQLDRAAVLAANLIDEGERRDLDVFRLL
                     GGTQQAAVSAVSALGGWCFAWQTIFHHQKSRFPGE"
     gene            2232..2756
                     /locus_tag="VF874_10115"
     CDS             2232..2756
                     /locus_tag="VF874_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176411.1"
                     /translation="MKLPVRHHRPLAALGAEDPDPTALSNHVATMRTFVETFRMVGLN
                     VYAAFHDGVVARLLTAAGQPEQARAHLDTALQLARDTGVRYYDAELLRLRAHTQVDPD
                     PRRADIRAALQLARRQGAPLFELRAALDDVELRGQAARGALTDSVGRMPADSGSPELA
                     RARTILGLTDPAIG"
     gene            2939..3721
                     /locus_tag="VF874_10120"
                     /pseudo
     CDS             2939..3721
                     /locus_tag="VF874_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215400.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
BASE COUNT          553 a         1182 c         1335 g          651 t
ORIGIN      
        1 cgcagtggtc aagggtgcgg aggggccgat cacttaccac ctggtgttgg gtgagtgcgc
       61 cgacgcgggc cgtgtgggcg tgggccgtgg tccgcttatc ggccgtgacc gtgagttggc
      121 gcggctgcag aggagctggg cccgcgcgca ggcgggcacg ctgaccgtgc cgggtgtggt
      181 gttccgtggt gagcccggca tcggtaagtc tcggttggcc gcggcggccg tcgagctcgc
      241 cgaggactcg ggtgcggtgg tgttggagtt gatcgggtca ccgtttcaca ccgacaccgg
      301 gctgcatccg gtgcgcaccc tgatcgagcg ccgctgtggc atcgatcgga tgaccgatcc
      361 ggccgagcag ctgcgcatgc tgcacgccga ggtggcggcc cggtcgctgg atccggtgcg
      421 gctggtgccg ttgttggccc cggtgttggg catcgcgccc gccgccggct atgagccggt
      481 gcccgctgag ggccgcaagc tctatgagct gatcggcgag gcggtgcggg agtacctgct
      541 ggcgtgtctg ggtgatgatg ccgggctgct ggtcaccgag gacgtgcagt ggtttgacgc
      601 gtcgaccctg gaggtgctcg gctgcctgct gactgcggcg cgagggcggc tgctggtggt
      661 gatcgccggg cggcccgggg gttggctgcc gggcggctgg cgcgtcaagg tttttgacct
      721 tcaaccgttg accgatgagc aggccgacag gctgatcacc gccttggatc ccagcctccc
      781 ggcgaaggac cgggcggcgg tgtgcgcccg ctgcgacggt gtcccgctct acatcgagca
      841 gcttgtccgg ggggcgcgtg agaaaccggc cggcgactcg gccgagacac gggtgccgga
      901 tgcgctgtat gagccgttgc tcgcccggct acacgcgagt gccgaggtgg tgccggtggt
      961 ggaggcggcc gggatcatcg gccgccatgt cgatcgcggc ctgttgtgtt cgctggtcgc
     1021 cctaagcgag gacgaggtcg accacatcat cgacgaactc gaagacgcgc tggtattgga
     1081 gccgtctgga accgacgggt ggcaatttcg ccacgaattg ctgcgcgaag tggccgccga
     1141 gttggcgccc cccagcgtgc gccgggggtt gcacgccaag gtggccgatg ccctggtgca
     1201 ccgtgtggac ggtggagacc cggactgggg gttggttgcg cgccactacg agcaggcgca
     1261 gcggttcgac tcgtcggcct gggcctacca gcaggcctcc gcggctgccc ggcggcgcgg
     1321 ggcgctggcc gaggcgcgca cctacctgac cctcgcattg gccgggctgg accgtgccac
     1381 atccggcccg gaccgcgacc ggcgggaaat cgcgctgcgg ctggagcggg gattcctcac
     1441 cacggccgta tcgggctata ccgaagtcgg tgacttcgag cggtgcctgc agctgtgggg
     1501 gaccgagctg tgggacgacg acctggtcgt aacattgttc gccctggcgg gctattacac
     1561 cctacgcgct gacctgcgcc gggccgccca ggtgctcgag tcgctgcgcg cgggggccgg
     1621 ggaggggccg ccctggttgc gtccggtgat cgatggcggg ttcggggtgg tggcgtggct
     1681 tcgcggcgag ttcgatgccg ccggttccta cctcgagcag gcgacgaggg ggctggcctc
     1741 ggcagacctg cacgagatcg acgcggtgtg gatccagccc gacgagccgg tcgcatcggt
     1801 gcacgtctac ctggccttgg tccgcctcgt gcgcggcgat ttcagcggcg ccgagactga
     1861 gctggcgcag gcggcacgcc gggccgacga gcttggcttc ccgcagggcc cgtacgtgcg
     1921 agccatggcg tgctacgtag agatctggct gcgcatcgaa ggcggtcagc tcgaccgggc
     1981 agcggtgctg gcggccaacc tgatcgacga gggcgagcgg cgcgacttgg acgtatttcg
     2041 gctgttggga ggtactcagc aggccgccgt cagcgccgtc agcgccctag gtggctggtg
     2101 ttttgcatgg caaacaatct tccaccacca aaaatcgcga tttccgggag agtgatttgg
     2161 tcggcaggtc gccgaaagtc gctcagcgtc cgttctgtgc ggtgccggtt ttggccgatt
     2221 tcgccttgcg cgtgaaactg cccgttcgac accaccgccc tctagccgcg ctgggtgccg
     2281 aggaccccga cccgaccgcg ctgtcgaatc acgtggcgac gatgaggacg ttcgtcgaga
     2341 cctttcgcat ggtcgggctg aacgtgtacg ccgcgttcca cgacggtgtg gtggcgcggt
     2401 tgttgaccgc cgccggccaa cccgagcagg cccgcgccca cctggacacc gctctgcagc
     2461 tggcgcgtga caccggagtg cgttattacg acgccgaact gctgcggctg cgcgctcaca
     2521 cccaggtcga ccccgacccc cgccgagccg acatccgcgc cgccctccag ctcgcccgcc
     2581 gccaaggcgc tcccctgttc gaactgcgcg ccgccctcga cgacgtcgaa ctacgcggtc
     2641 aggccgcgcg cggcgctctc accgactcgg ttgggcgcat gcccgccgac agcgggtcgc
     2701 cggaactggc ccgagcgagg acgattttgg ggctaaccga tccagcgatc gggtagccga
     2761 ttcctggcgc gggcacgact ggctcttgcc aatcgaaaag tgacccagct cacgtgacat
     2821 gtgtcaggtg cgcgtcgccc aaacttgcca acctgggtct agacagttat ccagtacccg
     2881 cggtaccgtt tctggacaca aaaggcatcg atgtaaacct cacaggggga gggttcggat
     2941 ggctcgaacg cgcattgtca ggcgctggaa gcgcaacatg gaagtcggcg acgaccgcga
     3001 gtacgtcgag acgctggcca ccctctccga gggatccgtg cgccgcaact tcaaccctta
     3061 caccgacatc gactggaccg acccggagtt cgcggtcacc gaaaacgacc cgcgctgggt
     3121 gctgccggcg acagacccga ttggccggca tccctggtac cagggatgcc gctggataag
     3181 cagatcgcga tcgggatgtg gcgtcaggcc aacgtcgcga aggtcggtct gcacttcgag
     3241 agcatcctga tccgcggcct gatggaatac acgttctggg tgcccaacgg atcacctgag
     3301 taccgctact gcctgcacga gtccgtcgaa gagtgcaacc acacgatgat gttccaggag
     3361 atggtgaacc gcatcggcgt ggacgtgccg ggcatgccgc ggttgctgcg ctgggtgtcg
     3421 ccactcatcc cgctcgccgc cagcccactg ccgatcgtgt tcttcttcgg ggtgctggcc
     3481 ggtgaggagc ccatcgacca cacgcagaaa aacgttctgc gtgagggcaa gtcgctgcac
     3541 ccgatcatgg agcgggtgat ggcgattcac gtcgccgagg aagcgcgtca catctgcttt
     3601 gcccatgagt acctgcgcaa gcgagttcca aacgtgcgcc gacgtcctaa gttctggctg
     3661 tcgttgcacg ttccgatcat catgcgggtg ctgtgttcgg cgatcgtgat tccgccgcgc
     3721 a
//
LOCUS       DASWBF010000279        12469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_686896,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000279 DASWBF010000000
VERSION     DASWBF010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686896"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VF874_10125"
     CDS             complement(<1..475)
                     /locus_tag="VF874_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX9176412.1"
                     /translation="MTERARVGGATVQWGVATHVGAVHEQNQEQNQDSYCAKPPVFVV
                     ADGVGGHAGGEVASRVAVESMLPLVDRADVTDEMVARCLADARERIGRISAERGRPPG
                     TTLTGVIATAADDVPSWLVVNIGDSRTYLLNASGLQQLSVDHSVVQQLIDVGAVEP"
     gene            complement(520..1587)
                     /locus_tag="VF874_10130"
     CDS             complement(520..1587)
                     /locus_tag="VF874_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX9176413.1"
                     /translation="MRWTHGLPSVAGVLTEDQCRQTALSIAAAQESSGAIPWFPGGHT
                     DPWDHVECAMALTTAGLMGPARAAFEWCRQMQRPDGSWPIQLRAGAVEDANSDSNFCA
                     YVAAGVWHHVLLTGERGFATTMWPVVSAAIDFVLDLQIGYGEICWARSESGPMPEALL
                     TGCASVFHSMRCALALADFLGEPQPEWEVAVGRLGHAIVAHPDAFEEKDRHSMDWYYP
                     ILGGAMRGPAATARIERRWRDFVVTGLGVRCVADRPWVTGAETCELVMALDAIGYHGA
                     AMEQFTAMQHLREEDGSYWTGLVFADGKRWPEERTTWTGAAMILAADALSGATPASGL
                     FRGAELPLGLQTDFDCICVTT"
     gene            complement(1584..2351)
                     /locus_tag="VF874_10135"
     CDS             complement(1584..2351)
                     /locus_tag="VF874_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9176414.1"
                     /translation="MLTVDFDRLGIGPSTKVIDVGCGAGRHAFEAYRRGADVVAFDQD
                     AVELQGVDTILTAMAEGGQVPASATAEAVVGDALALPYADGTFDCVIASEILEHVPDD
                     DAAIAELIRVLKVGGSLAVTVPRWFPERVCWWLSDKYHSNEGGHVRIYRASELRAKFL
                     GAGLTLTHTHHAHGLHSPFWWLKCAVGVEQSDHPAVTAYHKMLVWDLTRQPRVTRMAE
                     AVLNPLVGKSVAMYFAKPQVAQPVQPVVERQAARGSA"
     gene            complement(2345..3595)
                     /locus_tag="VF874_10140"
     CDS             complement(2345..3595)
                     /locus_tag="VF874_10140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9176415.1"
                     /translation="MRIALLSYRSKTHCGGQGVYVRHLSHGLVELGHEVEVFSGPPYP
                     EVLDPRVRFTEVPSLDLYREPDPFRVPWPNEIRSRFDLLELLTMWSAGFPEPRTFSLR
                     AARLLAGRAGDFDVVHDNQSLGTGLLKIAATGLPVVATVHHPITRDRILDLAAARWWR
                     KPLVHRWYGFLKMQQEVSRHIPELLTVSSSSAADIAADFAVSPDQLHVVPLGVNTERF
                     KPSSRPRRPGRVIAIASADIPLKGVATLLHAVARLRAERDLELQLVARVEPNGPTDKL
                     IAELGISDIVHIKSGLPDDELAALFASAEVACIPSLYEGFSLPAVEAMASGTPVVASR
                     VGALPEVLGTDGACAELVAPADIGELTRALGDLLDSPEKRRSLGEAGRRRALEVFSWE
                     SVAAQTVRVYKRAIARSGKGTEQC"
     gene            3890..4783
                     /locus_tag="VF874_10145"
     CDS             3890..4783
                     /locus_tag="VF874_10145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9176416.1"
                     /translation="MTEEHLLEGRAAVVVGGSRGVGRAVSELLAACGAAVVVNGRDAD
                     AADEVASAITRSGGRAVAHAGSPADEATADALIETCMIEFGRIDILVNCAGIAGRHDS
                     SILNTTTDEFAEFLEAHLWTVFQTCRAAAPRMATQRSGAIVNTSSFAFLGDYGGTGYP
                     AGKGGVNGLTMAIAGQLKEHGVRANVVCPGAKTRLSSGPEYEAHITDLHSRGLLDDAT
                     KQIALDAPPPEYAAPIYAYLASDMAADVTGEIFVAAGGYVGKFNRPEQVLLGYRDHTD
                     FPPWSVTELHKLVRQERPKHA"
     gene            4776..5528
                     /locus_tag="VF874_10150"
     CDS             4776..5528
                     /locus_tag="VF874_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9176417.1"
                     /translation="MLDISDDNRVRTLTLNRPEALNAFNEALYDETAQALRAAADDPD
                     VAVVVITGAGRAFSAGADLVEMQANITNPRENAGQHGFFTMLEALASFPKPLICAVNG
                     VGVGIGATILGYADLAFMSTDARLKCPFTSLGVAPEAASSYLLPRQVGRQNAAWILMS
                     SEWISAQEALEMGLVWKVCEPGDLLAETYRHANTLATKPIPSLMAVKTTMAAPLRPQI
                     DAAIERETAHFAELVGAAANAAALNAFTGRDD"
     gene            5537..6202
                     /locus_tag="VF874_10155"
     CDS             5537..6202
                     /locus_tag="VF874_10155"
                     /inference="COORDINATES: protein motif:HMM:NF025398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX9176418.1"
                     /translation="MNIINRLIGSVFVLAVFGVTGCTQVVSGAAMAPTSVGPTDIKAL
                     LLDEDEVNSIMETSDLVLAESGDGPDDTIDACPSECHGGIYMSGEKEYGSTDFTEMRW
                     EAVASESGSVVQSVAKFPSSSDAAAFVEDQTTSWDSCRKVTITAVNKEDGTKNRYQVR
                     SVSAQHDKVTALTVLADMDWQCQHVLQAVSSFVLDISACGSNISDQAETVASEVASKV
                     DNK"
     gene            complement(6227..7804)
                     /gene="fadD1"
                     /locus_tag="VF874_10160"
     CDS             complement(6227..7804)
                     /gene="fadD1"
                     /locus_tag="VF874_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD1"
                     /protein_id="HEX9176419.1"
                     /translation="MTETVQQLLRGRINDDSVGVKHEDREWTWGEHLHDASAQASALL
                     AIADPTRPMHVGVLLGNTPDMLTQMAAAGLGGYVLCGINTTRRGEALLADAQRADCQI
                     LVTDAEHRPLLDGLDLGRIRLVDTSSEEWRHRVDAAGELQPHRETTATDTFMMIFTSG
                     TSGNPKAVQVAHAMVLFAGQALVAKFELTRDDICYISMPLFHSNAVVGGWAPAVVAGA
                     AMVPAKFSASGFLSDIRRYGVTYMNYVGKPLAYVLATPERPDDADNPLRVAFGNEGSD
                     RDIVEFARRFDVHVDDGFGSTENAVIITREPETPKGSIGKGFPGVAIYNSETVTECPV
                     ARFDENGALTNADEAIGELVNTSGTGFFSGYYNDDAANAECMRHGFYWSGDLAYRDVD
                     GWIYLAGRTADWMRVDGENLAAAPIERILLRLPECNRVAVYAVPDENVGDQVMAAIVL
                     RDGATLTPSAFEAFLTKQPDLSPKAWPRYVRIAPDLPSTATHKVLKRALIAEGATAGD
                     GELWVRDARDTAYRRSG"
     gene            7852..9615
                     /locus_tag="VF874_10165"
     CDS             7852..9615
                     /locus_tag="VF874_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9176420.1"
                     /translation="MNFDTVIRNGRWFDGSGAPSAIRNIGIRDGHIAAISPDPLDETG
                     CGQVIDASGKWVLPGMIDIHTHYDVEVLEGPSLSESLRHGVTTILLGSCSLSTVHVDG
                     VDAGDLFGRVEAIPREHVIRAVDAHKTWKNCGGYVAALESLPLGPNVAAFIGHSDMRT
                     ATMGLDRATREDVRPTKSEQVRMERMLANALDEGFLGMSSQQLLFDKLDGDTCRSRTL
                     PSTYAKARELRRLKSLLRRSDRVLQSGPDIQNSVNLVSQIAQSLGIMRKPLKTSLLSA
                     ADIKAIPFVIHMMGPLARFVNKLGGNFRWQHLPVPFEVYADGIDLVIFEEFGSGAAAL
                     HLKEEVARNELLRDEKYRRQFRKDYESKYGPRVWHRDFFDAEIVECPDRSLVGKSFGQ
                     VGRERGLHPVDAFLDLVLEHGTAVRWHTTISNHRPDVLKKLARDPGIQMGFSDAGAHL
                     RNMAFYNFGLRLLRHVRDAEIAGAPFMSVEQAVHRLTGELADWYQIDAGHLRLGDRAD
                     VVVIDPKRLDASLDEYAEEKVDQYAGLSRMVNRNDDTVSAVFLDGKATDIVGTQRTGS
                     FLRAPTVSAVPVPVDPVAAAV"
     gene            9616..10062
                     /locus_tag="VF874_10170"
     CDS             9616..10062
                     /locus_tag="VF874_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020107973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9176421.1"
                     /translation="MGDVSATVHGLWQALSQRDWDAVQTFLADDCIYLDMPLGPTAAA
                     RGPLDIVKRLKIGIEPLAGYVNHDGLLVINGVDAIYEHSETWTWKSGETAVLPFVTVH
                     RVIDGKITLWKDYWDMGTLTAQAPAGWLETLMTADMSWVFDATGVI"
     gene            10236..11528
                     /locus_tag="VF874_10175"
     CDS             10236..11528
                     /locus_tag="VF874_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176422.1"
                     /translation="MSTVANLVDGSATIKESAEAFIADPLTFFDMSLTKMQSVPRDLL
                     EELQTEALSMRFERQKERIPTLSKLADRQGITRVREIDEVLPVLFEHTMYKSYPTYLL
                     EKGQFDKLTTWLNRLTPHDLSQLDTSGCDSIHSWLDLLCAETELDPISSSGTTGTMSF
                     TPRDKSDWRTFILGGFRIQLLQKFGEPPTESDLDEKIHVCWPSYRDGHTPFFRAPHYF
                     HQYFAMGSDDHFHPMFDTAGDTDVMFLAARLRAAQARGDSRVDVPQSLLDRRSEIEAM
                     ERGRPERSAAWMETLVSEQLSGKRVFLMAPAQLIYDVAVPGLAEGKKCNFASNSYITI
                     GLGGKGFVMPDDWFDVVKRFLNFDVDSGEFYAFSEQTGVSVRCEHGRCHLPPWLIPLI
                     LDAETSELLPRTGVQLGRGAFFDLAINGLWGGLITGDK"
     gene            complement(11676..12469)
                     /locus_tag="VF874_10180"
                     /pseudo
     CDS             complement(11676..12469)
                     /locus_tag="VF874_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2225 a         4018 c         4046 g         2180 t
ORIGIN      
        1 atggctcgac cgcgccgaca tcgatcagct gctggacgac cgaatgatcg acgctcagct
       61 gctgcagtcc agaggcattc agcagatacg tgcgcgagtc accgatgttg accaccaacc
      121 aggacggcac gtcgtcggcc gcggtggcga tcactccagt cagcgtcgtt cccggtggcc
      181 gcccgcgttc agccgatatc cgaccgatcc gctcgcgggc gtccgccaga catctcgcga
      241 ccatctcgtc cgtcacgtcg gcgcgatcga cgagaggcag catcgactcg actgccacgc
      301 ggctcgcgac ctcgccgccc gcgtgaccac caacaccgtc cgctacgacg aagacgggcg
      361 gtttcgcgca gtatgagtcc tgattctgct cttgattctg ctcgtgtacc gcaccgacat
      421 gggtcgccac accccactgc actgtcgcgc cgccgacgcg ggcacgctcg gtcacgccgc
      481 aatcttatgt tcgaaacggc cgatgcgggg gcgactgcct caagtagtca cacagataca
      541 gtcgaagtcg gtctgaagcc cgagcggcag ctccgcgccg cggaacaggc cgctggcggg
      601 tgtggcgccc gacaacgcgt cagcggccag aatcatcgcc gcgccggtcc aggtggtgcg
      661 ttcctcgggc caacgcttgc cgtcagcgaa aaccaaaccg gtccaatatg atccgtcttc
      721 ctcgcgcaga tgctgcatcg cggtgaattg ctccatggcg gcgccgtgat agccaatcgc
      781 gtccagcgcc atcaccagtt cacaggtttc ggcaccggtc acccacggcc ggtcagcgac
      841 gcaacgcacc ccgaggccgg tgacgacgaa atcgcgccat cgccgctcga tgcgggcggt
      901 ggcggcggga ccccgcattg ctccgccgag gatcgggtag taccaatcca tcgaatggcg
      961 gtccttctcc tcgaacgcgt cgggatgcgc gacgatcgcg tggcccaacc ggccgacggc
     1021 gacctcccac tccggctgcg gctcgccgag gaaatccgcg agcgccagcg cgcacctcat
     1081 gctgtggaac acgctggcgc agccggtcag caacgcctcg ggcatggggc cagattcgct
     1141 tcgggcccag cagatctcgc cataaccgat ctgcaggtcc aatacgaagt cgatcgccgc
     1201 actgaccacc ggccacatcg tggttgcgaa accacgctcg ccggtgagca gcacgtgatg
     1261 ccagaccccg gcagcgacgt aggcgcagaa gttgctgtcg ctgttggcgt cctcgaccgc
     1321 tccggcgcgc aactgaatcg gccaggaacc gtccggacgc tgcatctgcc ggcaccattc
     1381 gaaggccgcg cgcgccggcc ccataaggcc cgctgtggtg agcgccatcg cgcattccac
     1441 gtgatcccac ggatcggtgt gcccaccggg gaaccacggg atggcgcctg acgactcctg
     1501 cgcggccgcg atcgacaacg ccgtctgtct gcactggtct tcggtgagga cgccggcaac
     1561 agacggcagc ccgtgtgtcc acctcatgcg ctaccccggg ccgcttgcct ctcgacaacc
     1621 ggctgaaccg gttgcgcgac ttgcggttta gcgaagtaca tcgccacgct cttgcccacc
     1681 agcgggttga gcacagcctc ggccatccgg gtgaccctgg gctgtcgcgt caggtcccac
     1741 accagcatct tgtggtacgc cgtcaccgct ggatgatccg attgctccac tccgactgcg
     1801 catttcaacc accagaacgg agagtgcagg ccgtgcgcgt ggtgggtgtg ggtgagtgtc
     1861 aatccggcac cgaggaattt ggcgcgcaac tcgctggccc gatagatgcg aacgtggccg
     1921 ccctcgttac tgtggtactt gtcggaaagc caccagcaca ctcgttcggg aaaccaccgt
     1981 ggaaccgtaa cagccagtga gccaccaact ttcagcactc gaatcagttc ggcgatcgca
     2041 gcgtcatcgt ccggaacatg ctcgagaatc tccgaggcga tcacgcagtc gaatgtcccg
     2101 tcggcgtatg gcaacgccag cgcgtcgccg accaccgcct ccgcagtggc cgatgcgggc
     2161 acctgaccgc cctctgccat cgcggtcaag atggtgtcca cgccctggag ttcaaccgcg
     2221 tcctgatcga atgcgacgac gtcagcaccg cggcggtacg cttcgaatgc gtgccggcct
     2281 gccccgcaac ccacgtctat caccttggtc gacgggccga tgcccagccg gtcgaagtcc
     2341 actgtcagca ctgctcggtc cccttgccgg acctggcgat cgcccgcttg tacacccgca
     2401 ccgtctgagc cgccaccgat tcccagctga acacctccag ggcccgccgc cggccggcct
     2461 cgccgaggct gcgccgcttc tcgggtgaat ccaacaggtc ccccagtgcg cgggtcaatt
     2521 cgccgatgtc ggcaggcgcc accaactcgg cgcaggcgcc gtcggtgccg agaacctcgg
     2581 gcagcgcacc gacgcggctg gccaccacgg gcgtgccgct ggccatcgcc tccactgccg
     2641 gcagcgaaaa cccttcgtac agagacggta tgcacgcgac ttcggcggag gcgaacagcg
     2701 cggccagctc gtcgtctggg agtccgctct tgatgtggac gatgtcggaa atgcccagtt
     2761 ctgcaatgag cttgtcggtc gggccgttgg gttcgacccg ggcgaccagt tgcagttcga
     2821 gatcgcgctc ggcacgcaac cgcgccaccg cgtgcagcag cgtcgccacc cccttgaggg
     2881 ggatgtcggc gctggcgata gcgatgactc ggcccggccg acgcgggcgg gacgacggtt
     2941 tgaaccgctc cgtgttcacg cccagcggaa cgacgtgcag ctgatccggt gataccgcga
     3001 aatccgcggc gatgtcggca gccgacgacg acgacaccgt cagcaattcc gggatgtgac
     3061 gggacacctc ctgctgcatc ttcaggaagc cataccacct gtggaccaac ggctttcgcc
     3121 accaacgtgc cgcggccagg tccagtatgc ggtcacgggt gatcggatgg tgcacggtcg
     3181 ccacgaccgg caggcccgtc gcggcgatct tgagcagtcc ggttccgagg ctctggttgt
     3241 cgtggacgac gtcgaagtcg ccggctcgac cggcaagcaa ccgcgcggct cgcaggctga
     3301 aggtgcgcgg ctccggaaag ccggccgacc acatcgtgag cagctccagc aggtcgaaac
     3361 ggctgcgaat ctcgttgggc cacggcaccc ggaacggatc gggctcccgg tagagatcca
     3421 gactgggcac ctcggtgaat cgaacccgcg ggtcgagcac ctccggatag ggcgggccgg
     3481 agaagacttc cacctcgtgg ccaagttcga ccagcccgtg gctgagatgc cggacgtaga
     3541 cgccctgtcc tccgcagtgg gtcttactgc ggtaggacaa caaggcaatc cgcatgctca
     3601 ctctttccgc cgttgattgc tcgcctgact gcggcgcgcg atggcaccgt tgaaaacgaa
     3661 tctgccatat gaggtgcagc gatcggggcc cgggttatac agcgccatat ctgcaggccg
     3721 aacggccaca ggtgcagtgg gcgcggcggc ctgcgatcgc gtccttgcca tgcattgcga
     3781 acccggggcg tcggacggac gcggtcatgc gagactcgga cgatagctgg acatgtgtcc
     3841 ggactatagt tcgacaccca taagggcgca acgcgcgacg gaggcagcgg tgacggaaga
     3901 acacctgctg gagggccgcg ccgcggtggt ggtcggcggc agccggggtg taggcagggc
     3961 ggtgtccgaa ttgctggcgg cctgtggcgc cgccgtcgtg gtgaacggcc gggacgccga
     4021 tgcggccgac gaggtcgcct cggcgatcac gcggagcggg ggacgggccg tcgcccatgc
     4081 cggctcaccg gccgacgaag cgaccgctga cgcgctgatc gagacctgca tgatcgagtt
     4141 cggccggatc gacatcctgg tcaactgcgc gggcattgcc ggacgccacg actcgtcgat
     4201 cctgaacacc acgaccgatg aattcgccga gttcctcgag gcgcacctct ggaccgtctt
     4261 ccagacctgt cgcgccgcag cgccgaggat ggcgacgcaa cgcagcgggg cgatcgtcaa
     4321 caccagctcg ttcgcgttcc tcggcgacta cggcggcacc gggtatccgg cggggaaggg
     4381 cggcgtcaac ggactcacca tggcgatcgc ggggcaactc aaagagcacg gcgtgcgggc
     4441 gaacgtcgtg tgcccgggcg ccaagacccg gctgtcgagc ggaccggagt acgaggccca
     4501 catcaccgac ctgcattccc ggggcctgct cgacgacgcc accaagcaga ttgcgctgga
     4561 cgcgccgccg cccgagtacg cggcaccgat ctacgcgtac ctcgcgagcg acatggccgc
     4621 cgacgtcacc ggcgaaatct tcgtggccgc aggtggttac gtgggcaagt tcaaccgccc
     4681 cgagcaggtg ctgctgggct atcgcgacca caccgacttc ccgccatggt cagtcaccga
     4741 actccataag ttggtcagac aagagaggcc aaagcatgct tgacatttcc gacgacaacc
     4801 gcgtgcgcac gttgacgctg aaccggcccg aggcgctgaa cgcgttcaac gaggcgctgt
     4861 acgacgagac ggcacaggca ctgcgggccg cggccgacga ccccgacgtc gcggtcgtcg
     4921 tgatcaccgg cgccggccgg gcgttcagcg cgggcgctga tctcgtcgag atgcaggcga
     4981 acatcaccaa tccgcgcgag aacgccggcc agcacgggtt cttcacgatg ctcgaggccc
     5041 tggcgtcgtt tccgaagccg ctgatctgcg ccgtcaacgg tgtcggggta ggcatcggcg
     5101 ccacgatcct cggctacgcc gacctcgcgt tcatgtcgac cgacgcccgc ctgaaatgcc
     5161 cattcaccag cctgggtgta gcacctgaag ccgcgtcgtc ctatctgttg ccgcggcagg
     5221 tgggacgcca gaacgcagcc tggatactaa tgtcgtcgga atggatcagc gcccaggagg
     5281 cgctcgagat gggtctggtg tggaaggtct gcgagccggg cgatctgctc gccgaaacct
     5341 accggcatgc gaacacgctt gcgacgaaac ccattccgag cctgatggcg gtcaagacca
     5401 cgatggcggc cccgctgcgt ccgcagatcg atgccgcgat cgagcgggag acggcccact
     5461 tcgccgaact ggtcggtgct gcggcgaatg ccgcggcgct aaacgctttc accggacgcg
     5521 acgactaact caagaggtga acatcatcaa tcggttgatc ggttctgttt tcgtcctcgc
     5581 tgtcttcggc gtcaccggtt gcacgcaagt ggtgtccggg gcggcgatgg ctccgacctc
     5641 agtggggccg acggacatca aggctctgct tctcgacgag gacgaagtca attcgatcat
     5701 ggaaaccagc gatctcgtcc tggccgagtc cggtgatggt ccggacgaca ccatcgacgc
     5761 ctgcccgtcg gagtgccacg gcgggatcta catgagcggc gaaaaggagt acggcagtac
     5821 tgacttcacc gagatgcgtt gggaggcggt cgcatcggag agtggcagcg tcgtgcaatc
     5881 agtggccaag ttcccgtcgt cgtcggacgc cgctgcgttc gtcgaggacc aaaccacgtc
     5941 gtgggactcc tgccgaaagg tgacgatcac cgccgtgaac aaggaggacg gcacgaagaa
     6001 tcggtaccag gtgcgatcgg tttccgcgca gcatgacaag gtgacggcgc taaccgtgct
     6061 ggcggacatg gactggcagt gccagcatgt gctgcaggcg gtctcgtcgt tcgtgctcga
     6121 catcagtgca tgcgggtcga acatctccga ccaagccgaa accgtcgcgt cagaggtcgc
     6181 ttcgaaggtc gacaacaaat aaccctctgc gccacacggg ttttcgctag ccgcttcggc
     6241 gataagcggt atctcgggcg tcacgaaccc acagttcgcc gtcaccggcg gtcgcgcctt
     6301 cagcgatgag ggcgcgcttg agcaccttgt gcgtcgccgt gctcggcagg tcgggcgcga
     6361 tacgcacgta gcggggccac gccttcggcg acaggtcggg ctgctttgtg agaaacgcct
     6421 cgaacgccga cggtgtcaac gtagcgccgt ccctgagcac gatggccgcc atcacctggt
     6481 cgccgacgtt ctcgtcgggc accgcgtaca cagcgacacg gttacattcg ggcagccgca
     6541 acaggattcg ttcgatcggg gcggcggcca ggttctcgcc gtcgacgcgc atccagtccg
     6601 cggtacggcc ggccaggtag atccagccgt cgacgtcgcg gtatgccagg tcaccagacc
     6661 agtagaagcc gtgccgcata cactccgcat tggccgcatc gtcgttgtag tacccgctga
     6721 aaaatccggt acccgatgtg ttcaccagtt cgccgatggc ctcgtcggcg tttgtcaacg
     6781 ccccgttctc atcgaagcga gcgactgggc actcggtgac ggtttcactg ttgtaaatcg
     6841 ccacgcccgg aaagcctttg ccgatcgagc ctttcggcgt ctcgggttca cgagtgatga
     6901 tcaccgcgtt ctcggtcgac ccgaacccgt cgtcgacatg cacgtcgaac cgacgagcga
     6961 actccacgat gtctcggtcg ctgccttcgt tgccgaacgc gacgcggagt gggttgtcgg
     7021 cgtcgtcggg tcgctccgga gtggcgagga catacgcgag cggcttgccc acgtagttca
     7081 tgtaggtgac tccgtatcga cggatatccg acagaaagcc ggatgccgaa aacttcgcgg
     7141 gaaccatcgc cgcaccggcc accaccgcgg gtgcccagcc gccgacgacg gcgttggagt
     7201 gaaacagagg catcgatatg tagcagatgt catcgcgcgt cagttcgaac ttggcgacta
     7261 gggcctggcc cgcgaacagc accatggcgt gagcgacctg gacggccttg ggatttccgc
     7321 tcgtgccgga ggtgaagatc atcatgaacg tgtcggtggc ggtggtctcg cggtgcggct
     7381 gcagctcgcc cgccgcgtca actcggtgcc gccactcctc gctcgaggta tcgaccaggc
     7441 ggatccgacc gaggtcaagc ccgtcgagca gcggccggtg ttcggcgtcg gtgaccaaga
     7501 tctggcagtc ggcccgctgc gcgtcggcca gcagagcctc gccgcggcgg gtggtgttga
     7561 tgccgcacaa cacgtatccg cccagccctg cggcggccat ctgcgtcagc atgtcgggag
     7621 tgttgccgag cagcaccccg acgtgcatcg ggcgggtcgg gtcagcgatc gcgagcagcg
     7681 ccgatgcttg cgcggaggcg tcgtgcaagt gctcacccca tgtccactcc cgatcctcgt
     7741 gcttgacgcc aaccgagtcg tcgttgatgc ggccgcgcag cagctgctgg accgtctcag
     7801 tcacgcgcac tcctctcccc cgagcagacg cgctcatact aaggtgacct agtgaacttc
     7861 gacaccgtca tccgcaacgg ccggtggttc gacggcagcg gtgccccgtc ggccatccgc
     7921 aacatcggca tccgggacgg acacatcgcc gcgatctcgc cggacccgct cgacgagact
     7981 ggttgcggtc aggtgatcga tgcctccggc aagtgggtgc tgcccggaat gatcgacatc
     8041 cacacccact acgacgtcga ggtgctggag ggcccgtccc tgtcggagtc gctgcggcat
     8101 ggtgtgacca cgatcctgct ggggtcgtgc tcgctgtcga ccgtgcacgt cgacggtgtg
     8161 gacgccggcg acttgttcgg ccgtgtcgag gcaatccccc gcgagcacgt catccgcgcc
     8221 gtcgacgcgc acaaaacctg gaagaactgc gggggatacg tcgccgcgct cgaatcgctg
     8281 ccgctgggcc ccaacgtggc cgccttcatc ggccactccg atatgcgcac agcgacaatg
     8341 ggtttggaca gggccacccg cgaggacgtg cggccgacga agtcggagca ggtgcggatg
     8401 gagcgcatgc tggccaatgc gctggacgag ggctttcttg gaatgtcttc gcagcagctg
     8461 cttttcgaca aactcgacgg cgacacctgc cgctcacgaa cgttgccgtc gacgtacgcc
     8521 aaggcgcgcg agctgcgccg gctgaagtcg ctgctgcggc gcagcgatcg ggtgctgcag
     8581 tccggacccg acattcagaa ctccgtgaac ctcgtatccc aaatcgccca gtcgctgggc
     8641 atcatgcgca agccgttgaa gacgagcctg ctctcggcgg ccgacatcaa ggccatccct
     8701 ttcgtgatcc acatgatggg tccgctggcg cgtttcgtca acaagctcgg aggcaacttc
     8761 cggtggcagc atctgccggt gccgttcgag gtgtacgccg acggtatcga cttggtgatc
     8821 ttcgaggaat tcggttcggg tgcagcggct ttgcacctca aggaagaagt cgcgcgaaac
     8881 gaactgctgc gtgacgagaa gtatcgccgg cagttccgca aggactacga gagcaagtac
     8941 ggcccacgag tctggcaccg cgacttcttc gacgccgaga tcgtcgagtg tccggatagg
     9001 tcgttggtgg gcaagtcatt cgggcaggtg ggccgcgaga gggggctgca tcccgtagac
     9061 gcgttcctgg atctggtgct cgagcacggt accgcggtgc gctggcacac gacgatctcg
     9121 aaccaccggc cggacgtgct gaagaagctg gcgcgagatc ccggaatcca gatggggttc
     9181 tccgacgccg gcgcgcatct gcgcaacatg gcgttctaca acttcgggct gcggctgctg
     9241 cgccacgtgc gcgacgcgga aatcgccggc gcaccgttca tgtcggtcga gcaggcggtg
     9301 catcggctga ccggcgagct cgccgactgg taccagatcg acgccggcca ccttcggctg
     9361 ggcgaccgcg ccgacgtcgt ggtgatcgat ccgaagcgtc tcgacgcgtc gctcgacgag
     9421 tacgcagaag agaaggtgga ccagtacgcc gggctctccc ggatggtcaa ccgcaacgac
     9481 gacaccgtca gcgcggtgtt cctcgacggg aaggccaccg acatcgtcgg cacccagcgc
     9541 accggcagct tcctgcgggc gccgaccgtg tcagccgtcc ccgtgcctgt cgatcccgtc
     9601 gccgcggccg tctgaatggg tgacgtgtca gccaccgttc acggtctgtg gcaagcactt
     9661 tcgcagcgcg actgggacgc ggtgcagacc ttcctggccg acgactgcat ctacctcgac
     9721 atgccgctcg ggccgacggc ggccgcacgc ggcccgctcg acatcgtcaa gcggctcaag
     9781 atcgggatcg agccgctggc cggctacgtc aaccacgacg ggctgctggt catcaacggg
     9841 gtcgacgcga tctacgagca ctccgagacg tggacctgga agagcggtga aaccgccgtg
     9901 ctgccgtttg tgacagtgca tcgcgtgatc gacggcaaga tcacgctgtg gaaagactat
     9961 tgggacatgg gcacgctgac cgcgcaggca ccggcgggct ggctcgaaac gctgatgacg
    10021 gccgacatgt catgggtgtt cgacgcgacc ggcgtgatct gagcgcgtca cgttacaccc
    10081 tgccgctcgg aacaacctag ttcggagccg atagccagtt ttcgcaggta gtgaatcctg
    10141 ctgggcggtg ttgacaacaa acgttttcag agttatcgac gccggagcgg tggtaggttt
    10201 tggtgtgtcg cgagcccaaa atccggagga tgcccatgag taccgttgcc aatttggttg
    10261 acggcagcgc aacgatcaag gaatcggcgg aggctttcat cgcagatccc ctgacgttct
    10321 ttgatatgtc gctcacgaag atgcaatcgg tgcctcgcga tctcttggag gaacttcaga
    10381 cggaagcctt gtcgatgcgc tttgagcggc agaaagagcg tattccgacg ctgtccaagc
    10441 tggctgatcg gcagggaata acacgtgttc gcgagattga tgaagttctt cctgtgttgt
    10501 ttgagcacac aatgtacaag tcttatccga cttacctgct tgagaagggc cagttcgaca
    10561 aactgacgac ttggctgaac cggctgacgc cgcatgacct gagccagctg gatacctccg
    10621 gatgcgactc cattcactcc tggctcgacc tgctttgtgc ggagactgaa ctcgacccca
    10681 tttcctcttc cggcacgacc ggcacgatgt cttttactcc tcgcgataaa agcgattggc
    10741 gcacattcat tttaggcggt ttccgtattc agctgctgca gaaattcggc gagccgccca
    10801 cggaatctga tctagatgaa aaaattcatg tttgctggcc cagctatcgc gatggtcaca
    10861 cgccgttctt ccgagcgcct cactacttcc accaatactt tgcgatggga agtgacgatc
    10921 acttccaccc gatgtttgac acagcgggag acaccgatgt gatgttcctg gccgctcggc
    10981 tgcgtgctgc ccaagcgcgg ggggacagca gggtggatgt gccccagagc ctgcttgacc
    11041 ggcgctccga aatcgaggcg atggagcgtg gcagaccgga gaggtcggca gcgtggatgg
    11101 aaaccctggt ctcagagcag ctaagtggca agcgggtgtt tctcatggcc cctgcgcagc
    11161 tcatctacga cgttgcggtg ccaggcttgg cggagggtaa gaagtgtaac ttcgcatcga
    11221 attcgtatat cacaattggt cttggcggca aaggctttgt catgccagac gattggttcg
    11281 acgttgttaa gcgtttcttg aacttcgacg tcgactccgg cgagttctac gccttctccg
    11341 agcaaaccgg ggtcagtgtc aggtgtgagc atggccgctg tcatctcccg ccttggttaa
    11401 ttcccctcat tcttgacgcg gagacgagcg aactcttacc ccggacgggt gtacagctcg
    11461 gccgcggcgc ttttttcgat ctggcgataa atggtctgtg gggtggtctc atcaccggcg
    11521 acaagtagct gtacagcgac accagaggca cattccgatg cgatggactt tctaactaat
    11581 ttctaccggg gcttcgcccc agggctccgg cgttgttgac tagcaagctt gtgagctggg
    11641 gattcggtgg tctgtgagca ggtcgaggga gtcgcctacg gtgagggcgg tactgcggca
    11701 ggcttcggcg atgttggtgg ctccacgaag ccggtgcagg ttgacgacgg tgttgcggac
    11761 cgtggccgtg acctgtggac cggcgccggt gcggacggtc gagcggtcct cgtcgaacgt
    11821 cacgtcgcgc acccagtgga cgctgttctc gattccccag tgctggcgca gccaggcggt
    11881 gatcgcggcg gggcgagcct gttcgaacgg caggctgcag attgcgtaga ccacctcgac
    11941 ggtgcgggca ccggtggcga tcacgatgcg ttcgcgggtg atctgaacga cctgtctggc
    12001 gtaggggaat ccgatgccgc gggcggcggt gaggatcttc agggtgcgag tttcgcggcg
    12061 gccgtggccg cggtcgtcgt cggtgcagac aaccgggacc tcgacccacg ggagtgcggt
    12121 gatccgggcc agcagcttgg cctggtttga cttgacgacc atcaggtagt gcgatttcag
    12181 ggtgccgcag atcagtgtcg cggtcgcgcg ctgggtgtgc atcgcgtcca ccgttaccag
    12241 cagccccatc cggcgcagcg accggagaag ctttcgtacg caggggatct cgttgctctt
    12301 ctcggccact gcgagctgcc cgaggacgag ccgggcgcgg tgggcgaaca ccgacaccag
    12361 atgcgcggcc gcggctccgg cgcggcgtgc accgcgtagg ctcttgccgt ccaatgcgac
    12421 cgccaacggc tcaccggccg cagcggcctg ctcggccgcg agcgccatg
//
LOCUS       DASWBF010000280         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1213846,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000280 DASWBF010000000
VERSION     DASWBF010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213846"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="VF874_10185"
     CDS             complement(<1..1613)
                     /locus_tag="VF874_10185"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX9176423.1"
                     /translation="MNWRFPVWRAGVSGVASLATATGPDVPVTVFDSVRTTPRRARGA
                     SLADVADNDVVYAIPLKTPVTAPGPPARNALGTAPVGTFPTGVAVSPDGNRVYVANSG
                     DDSVSVIDAENGNAALTIAVGHAPYGIALTSDGRKAFVANASGNSVSVIDTQAQVVTA
                     TIAVGPNPYGVAVDPDGERLYVTNQADGTLTVIDAVKSTIVVGGAPTGVAVSPEGRTA
                     YVVDNESGRLVVVDTEEGVVTATVRVGSQPAQVAITPDGSRAFVTNTGSGSVSLIDLA
                     GSDGEATVTETIPVGAHPIGVAVCADGRFAYVTALDASRLSGTVTIIDTATGMTSTIH
                     AGAPYGVAADPAGDYAYVTDFRANAVSLIEAAAGPERPRHEAVRAITTDRATHSVTAD
                     PDMARAFVIRSDDDAIEVIESSGESVTVEVGAYPSALVPSRDGTRAYVTNYEDRTLSI
                     IDMDPLSDACNTVTATLDVGLCWATGVQLSGDGNRAYAVDEVDGHVSVIDTAVGSPNR
                     DTVVGHVELGEPPAALRIAPNGRWAYAIN"
     gene            1921..>3797
                     /locus_tag="VF874_10190"
     CDS             1921..>3797
                     /locus_tag="VF874_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX9176424.1"
                     /translation="MAPTGNDARTERQKLEKVVIRFAGDSGDGMQLAGDRFTSDAALF
                     GNDLATQPNYPAEIRAPQGTLPGVSSFQIQIADYDILTAGDRPDVLVAMNPAALKANI
                     ADLPRGGLVIANSDEFTKRNLAKVGYDANPLESGELSDYVVQAVPMTTLTLGAVEEIG
                     ASKKDGQRAKNMFALGLLSWMHGRQIEPQETFIREKFVRKPDIAEANVLALRAGWNYG
                     ETTEAFGTTFEVAPAKLPPGEYRQISGNTALAYGIIVAGQLADTKVVLGSYPITPASD
                     ILHELSKHKNFNVITFQAEDEIAGIGAAIGASYGGALGVTSTSGPGISLKSEALGLAV
                     MTELPLLVIDVQRGGPSTGLPTKTEQADLLQVLFGRNGESPIAVLAARSPSDCFTVAI
                     EAVRIALTYRTPVILLSDGAIANGSEPWRIPDVSTFEPIDRNFAQAGEDFQPYARDPE
                     TLARQFAIPGTPGLEHRIGGLESANGSGNISYEPDNHDLMVRLRQAKIDGIPVPDVEV
                     DDPTGDAELLLIGWGSSYGPIGEACRRARRKGIKVAHAHLRHLNPLPANLGDVLRRYR
                     TVVCPEMNLGQLALLLRGKYLVDVQSVSKVKGMAFLADEVEDIIDSALDGSLAEKESD
                     KT"
BASE COUNT          663 a         1271 c         1244 g          619 t
ORIGIN      
        1 tggttgatgg cgtacgccca tctgccattg ggcgcgatcc gcagcgctgc cggcggctcc
       61 ccgagttcga cgtgtccgac gacagtgtcg cgattcgggc tgccgacagc ggtgtctatc
      121 accgacacgt ggccgtccac ctcgtcgaca gcgtatgcgc ggttgccgtc accgctcagc
      181 tgcaccccgg tggcccagca cagccccacg tcgagcgtcg cggtgacggt gttgcacgcg
      241 tcgctgagcg ggtccatgtc gatgatggac agggtgcggt cctcatagtt ggtgacgtac
      301 gcgcgggtcc catcacggct cggcaccagc gccgaggggt atgcgcccac ctccaccgtg
      361 acggactccc cggacgactc aatcacctcg attgcgtcgt catcgctgcg aatcacgaag
      421 gcgcgagcca tatctgggtc ggcagtcaca ctgtgggtcg cgcgatcggt tgtgatggcg
      481 cggacggctt cgtgtcgagg ccgctcgggt cctgccgccg cctcgatcaa cgacacggcg
      541 ttagcgcgaa agtctgtgac gtacgcgtag tcaccagccg gatcagccgc aacaccgtac
      601 ggtgcgcccg cgtggatggt gctcgtcatg ccggtcgcgg tgtcgatgat cgtcaccgtg
      661 ccggacagcc gcgacgcatc gagtgcggtg acgtaggcaa agcggccgtc tgcgcacacc
      721 gccacaccga tcggatgggc gcccaccggg atggtttcgg tcaccgtcgc ctcgccgtcg
      781 gacccagcca ggtcgatcag cgacaccgac ccacttccgg tgttggtgac gaacgcccgc
      841 gacccgtcgg gagtgatcgc gacctgggcg ggctggctgc cgacccggac ggtcgcggtg
      901 acgacccctt cttcggtatc gaccaccacc aggcggccgc tctcgttgtc gacgacgtag
      961 gccgtgcgtc cttcgggact gacggccacg ccggtgggtg cgccgccgac gacgatcgtg
     1021 gacttgacgg cgtcgatcac ggtgagcgtt ccgtcggcct ggttggtgac gtacaggcgc
     1081 tcaccgtctg gatccaccgc gacgccgtac gggttcggtc cgaccgcgat cgtcgccgtg
     1141 accacctgcg cctgcgtgtc gataaccgac accgagttgc cggaggcgtt ggctacaaag
     1201 gcttttcgcc catcggaggt cagcgcaatc ccgtacggtg cgtgacctac cgcgatggtc
     1261 aacgcggcat tcccgttctc ggcgtcgatc accgacacgg agtcgtcacc gctgttggcc
     1321 acgtacaccc tgttgccgtc gggactaacc gcaaccccgg tcgggaacgt ccccaccgga
     1381 gcagtcccga gcgcgttccg cgccggcggc cccggagctg taaccggcgt cttcagcggg
     1441 atggcgtaca ccacatcgtt gtcggccacg tccgcgaggc tggcgccgcg ggcgcgccgc
     1501 ggtgttgtgc gcaccgagtc gaacaccgtt acggggacgt ccggaccggt ggcagttgcc
     1561 aacgaagcga cgccggacac gccggcccgc cacaccggaa accgccagtt catcaccagt
     1621 gagcatagga tttgacgagc cggataaaag gggttatgga cgaggtggtc gatcgtcttt
     1681 cgccagtcgg ccgaagtgct agccgacagt aagcgggtct cttagaaatt gcatcaggaa
     1741 aaacgtgacg aaaaccacac tttcagccgg ctccccggag tgtcgtggcg ctgaactgcg
     1801 actactgcgg aacaatgagc gattgcacgc gtcgcgcggg taaaggctag actaagaaag
     1861 tgccataatc ggtactagca gtgacatcaa cttcgcggct tgggcgaaag gtggtttgtc
     1921 gtggcgccga ccggcaacga cgcccgaacg gaacggcaaa aactcgagaa ggtcgtcatt
     1981 cgcttcgccg gtgattccgg tgacggcatg cagctggctg gggaccgctt cacctccgac
     2041 gccgcgcttt tcggcaatga ccttgccacc caaccgaatt accccgccga gatccgtgcg
     2101 ccccaaggca cgcttcccgg cgtttcgtcg tttcagatcc agatcgccga ctacgacatc
     2161 ctgactgcgg gcgaccggcc cgacgtcctg gtcgcgatga acccggccgc gctgaaagcc
     2221 aacatcgccg acctgccccg cggcggcctg gtgatcgcga actccgacga gttcaccaaa
     2281 cgcaacctcg cgaaggtcgg ctacgacgcc aacccgctgg aatccggcga gctatcggac
     2341 tacgtcgtgc aagccgtccc gatgaccacc ctgacgctgg gtgccgtcga ggagatcggc
     2401 gcgtcgaaga aggacggcca gcgcgccaag aacatgttcg cgctcgggct gctttcgtgg
     2461 atgcacggcc gccagatcga gcctcaggag accttcatcc gggagaagtt cgtccgcaag
     2521 cccgacatcg ccgaggccaa cgtgctggcg ttgcgcgccg gctggaacta cggcgaaacc
     2581 accgaggcgt tcggcaccac gttcgaggtc gccccggcca agcttccgcc gggggagtac
     2641 cgccagattt ccggcaacac cgcgctggcc tacgggatca tcgtcgccgg ccaactcgcc
     2701 gacaccaagg tggtgctggg cagctacccg atcacgccgg cgtcggacat cctgcacgag
     2761 ctgtccaagc acaagaactt caacgtcatc acgttccagg ccgaggacga gatcgccggg
     2821 atcggcgcgg cgatcggcgc ctcgtatggt ggcgcgctgg gcgtcacgag cacgtcgggc
     2881 cccggcatct cgctgaagtc cgaggcgctc ggactcgcgg tgatgacaga gctgccgctc
     2941 ttggtgatcg acgtgcagcg cggcggcccg tccaccggcc tgccgaccaa gaccgagcag
     3001 gccgatctgc tgcaagtgct gttcgggcgt aacggggagt ccccgatcgc ggtgctggcg
     3061 gcgcgctcgc cgtcagactg tttcaccgtc gcgatcgagg cggtgcgcat cgcgctgacg
     3121 tatcggacgc cggtgatcct gctgtccgac ggcgcgatcg cgaacggctc tgagccgtgg
     3181 cgcattccgg acgtcagcac gttcgagccc atcgaccgca acttcgcgca ggcgggtgag
     3241 gacttccagc cctacgcgcg cgatcccgaa acgttggccc gccagttcgc gatccccggc
     3301 acaccgggcc tcgagcaccg catcggcgga ctggagtcgg ccaacgggtc gggcaacatc
     3361 tcctacgagc cggacaacca cgacctgatg gtgcggttgc gccaggcgaa gatcgacggc
     3421 atcccggtgc ccgatgtcga ggtcgacgat ccgaccggcg acgccgaact gctgctgatc
     3481 ggctggggca gcagctacgg gcccatcggc gaggcctgcc ggcgcgcccg gcgcaagggc
     3541 atcaaggtgg cgcacgctca tcttcgccac ctgaacccgc tgccggccaa cctcggtgac
     3601 gtgctgcgcc ggtaccgcac cgtggtgtgc ccggagatga acctgggcca gctggcactg
     3661 ctgctgcgcg gaaagtatct ggtcgacgtc cagtcggtga gcaaagtcaa aggcatggcg
     3721 tttttggctg acgaggtcga agacatcatc gactccgccc tggacgggtc gctggcagaa
     3781 aaagaaagcg ataagac
//
LOCUS       DASWBF010000281         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_28885, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000281 DASWBF010000000
VERSION     DASWBF010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28885"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VF874_10195"
     CDS             <1..201
                     /locus_tag="VF874_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176425.1"
                     /translation="AVRPGQRVALKNGWMVVQQIWGINSMGRIWGSGRDFVIAVLTNG
                     SPTKNVGIATVEGGTRVAMRGL"
     gene            complement(356..1345)
                     /locus_tag="VF874_10200"
     CDS             complement(356..1345)
                     /locus_tag="VF874_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX9176426.1"
                     /translation="MPDLVAALSTKSACRLFGKSRATHYRRRLPPLRRPPMQPRPTPP
                     NALSEQERAAVLAVLASDRFVDKSVAYVWATLLDEGVYLASQSTMHRLLREAGQAGDR
                     RRQASHPTRTPPELVARKPGDVWSWDITKIKGPARAEWYQLYVILDIFSRYVVGWTVA
                     PGESGALATALIAAAIAEHGVPGSVHADRGTSMTSKPVAQLLLDLGVARSHSRPHVSN
                     DNPYSEAQFKTLKYAPVFPERFGSLADAKEFCRLFFTYYNSEHRHCALGLHTPASIHF
                     GAAAEIRQQRAVTLSAAFEANPLRFRGRCPQPPPLPQAAWINKPTPEALIQTN"
     gene            complement(1357..1734)
                     /locus_tag="VF874_10205"
     CDS             complement(1357..1734)
                     /locus_tag="VF874_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9176427.1"
                     /translation="MGTRGERPRRRAFTAEYKRRILREYEDATADGAKGALLRREGLY
                     YSQIQDWRRARDAGELASLEPRTRTPQRSADEVENERLRKKVERLEGELAKQKAAVEI
                     LGKAHELLELLSESADTEPPSMR"
BASE COUNT          340 a          714 c          610 g          332 t
ORIGIN      
        1 gccgtccggc cgggtcagcg ggtggccctc aagaacggct ggatggttgt ccaacaaatc
       61 tgggggatca acagcatggg acggatctgg ggctcgggcc gcgacttcgt gatcgccgtt
      121 ctcaccaacg gcagtcccac caagaacgtg ggtatcgcca cggttgaggg cggcacacgc
      181 gtcgcaatgc gaggcctgta gggggcgctg tcgcaccctg cgcggctgag gagggcaagg
      241 cgctgcccaa gacaaacgac accaccccgg accgggactg tgggtcgctc actcacccag
      301 gtcagtgcgg cgctgccggg acggaaggtg tcaagccggt tgagacaaga tcttcttagt
      361 tcgtttggat gagcgcttcg ggtgtcggct tgttgatcca ggcagcctgg ggcagcggcg
      421 gcggctgtgg gcatcgaccg cggaaccgca gcgggttcgc ctcgaacgcg gccgacaggg
      481 tcacggcacg ctgttgccgt atttcggcgg ccgcgccgaa atgaatcgac gcgggcgtgt
      541 gcaaacccag ggcgcaatgc cggtgctcac tgttatagta ggtgaagaac aaacggcaga
      601 actctttcgc atcggcgagt gagccgaacc gttcaggaaa caccggcgca tacttcaacg
      661 ttttaaactg ggcttcgctg tacggattgt cgttagacac atgcggccgc gaatggcttc
      721 tcgccacacc cagatcgagc agcagctggg cgaccggttt gcttgtcatc gatgtccccc
      781 gatccgcatg gaccgagccc ggaacgccgt gctcggcgat cgccgccgcg atcagcgcgg
      841 tggcgagcgc gccggactca cctggcgcga ccgtccaccc cacgacatag cggctgaaaa
      901 tgtcgaggat cacgtacagc tgataccact ccgcccgcgc cgggcctttg atcttggtta
      961 tgtcccatga ccacacatcc cccggcttgc gagccaccag ctccggaggc gtccgcgtcg
     1021 gatgcgaggc ctgccgccgg cggtcgccgg cctgccccgc ctcgcgcagc agccgatgca
     1081 tcgtggactg cgacgccaaa tacacaccct cgtcgagcag cgtcgcccac acgtaggcga
     1141 cgctcttgtc gacgaaccgg tccgaggcca acaccgccag caccgccgcc cgttcctgct
     1201 cggacaacgc gttcggcggc gtcggccgcg gctgcatcgg cggccgacgc agcggcggca
     1261 gccgccggcg gtaatgcgtc gcccgtgact tgccgaacag ccgacacgcc gacttcgtcg
     1321 acaacgccgc caccagatcg ggcagcgcag cgtcgactac ctcatcgacg gcggctcggt
     1381 atccgcgctc tcggagagca actccaagag ctcgtgtgct tttcccagga tctcgaccgc
     1441 ggccttctgc ttcgccaact caccttccag ccgctccacc ttcttacgca gccgctcgtt
     1501 ctccacctcg tccgcactgc gttgcggtgt ccgtgtccgt ggttccaacg acgccaactc
     1561 acccgcgtcc cgggcccgcc gccaatcctg aatctgcgag taatacaagc cctcccgccg
     1621 taacaacgcg cccttcgcac cgtcggcagt cgcgtcttcg tactcacgca gaatccgccg
     1681 cttgtactcg gccgtgaacg cccgacgccg cggccgttcc ccacgcgttc ccaccgaacc
     1741 atcatcggcc cgtccgggcc gaacatcggc gatcgtcatc gtcactacag gtcccgtccc
     1801 cgccctatga gctatgaacc cctaaccggg tgtctcatcc cagcgtgacg tacagggggc
     1861 gccgcatgac ggccgttccg gcgcacgccc gaacggctca cgctgtcccg gaggcgcccg
     1921 gtgcgcgcgc cccgtgctcc caggttgctg ccagtacgtc ccagccttgc agcacagggt
     1981 tcccgcaagg ctctca
//
LOCUS       DASWBF010000282         6306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1012375,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000282 DASWBF010000000
VERSION     DASWBF010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012375"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VF874_10210"
     CDS             <1..249
                     /locus_tag="VF874_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9176428.1"
                     /translation="IRDTLTANGWRIDFLGPTTYLANTSSMAGDPNNLPAQMRQQMSP
                     EQVELMKKTRERFAAIVPLLDDDRVHLPFTVVHAVRVD"
     gene            246..617
                     /locus_tag="VF874_10215"
     CDS             246..617
                     /locus_tag="VF874_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9176429.1"
                     /translation="MTGLFGPATANDWSFKPAAEVVKDGEDAVIRVELPGIDVEKDVN
                     VEVDKGALVIHGERRDEHSEEKDGRALREVRYGSLRRSFALPSHITGDAVTASYDAGV
                     LTVRVAGAYAGSEPKRIPITK"
     gene            complement(674..4270)
                     /locus_tag="VF874_10220"
     CDS             complement(674..4270)
                     /locus_tag="VF874_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX9176430.1"
                     /translation="MAGAGWQFWVDRGGTFTDVVARRPDGRLLTHKLLSENPSRYRDA
                     AVAGIRALLGTSAVGVPVPVARVEAVRMGTTVATNALLERKGDRTVLVITRGFGDALR
                     IAYQNRPRIFDRHIVLPEMLYERVVEVDERITAEGTVLRAPDLELLGEQLRRAHADGI
                     RAVAVVCLHSYLHPAHEREIGKLAEQIGFPQISLSSEVSPLMKLVPRGDTTVVDAYLS
                     PVLRRYVNQVADQMHGVRLMFMQSNGGLAEAGHFRGKDAILSGPAGGIVGMVRMSALA
                     GFDHVIGFDMGGTSTDVSHYGGEYERVFTTQVAGVRLRAPMLDIHTVAAGGGSILHFD
                     GTRYRVGPDSAGADPGPACYRGGGPLAVTDANVMLGRIQAAHFPAVFGPAGDQPLDDE
                     IVRRRFTDLAADIRARTGDDRPPEQVAEGFLRIAVANMANAVKKISVQKGHDVTRYVL
                     TTFGGAGGQHACAVADALGIRTVLVPPMAGVLSALGIGLADTTAMREQSVEMRLETPA
                     LERLTTVAESLEQTARAELLDEGIRVERIRVGRRVHLRYEGTDTPVPVELAEVDAMTA
                     AFEASHRATYSFLMDRPLIAEAVSVEAIGLTEQPDLSDLGDRAADTAGASETVRVYSG
                     GSWRDAPLCHRERMRQGDTVVGPAIVAEANATTVVDDGWRATMTASGHLLAERVVAPA
                     EPDAGTEADPVLLEIFNNLFMSIAEQMGFRLEATAQSVNIRERLDFSCALFDPDGDLV
                     ANAPHIPVHLGSMGTTVKEVIRRRAGEMKPGDVYAVNDPYHGGTHLPDITVVTPVFDA
                     AGGGILFFVASRGHHAEIGGITPGSMPADSSEIHEEGVLFDDWLLAENGQFREAATRR
                     LLTEARYPSRNPDTNLADLRAQIAANQKGVDEVGKMIDHFGIDVVQAYMRHVQDNAEE
                     AVRLVIDRLDDGDYRYRMDSGASIVVRITVNRATRSATIDFTGTSPQLGTNFNAPSSV
                     VTAAVLYVFRTLVAADIPLNDGCLRPLHIVIPEQSMLAPTYPAAVVAGNVETSQAITG
                     ALFAALRVQAEGAGTMNNVTFGNERHQYYETVGSGSGAGDGFDGASVVQTHMTNSRLT
                     DPEVLEWRFPVLLREFAIRRDSGGAGRWHGGDGAVRRIEFTEPMTVSTLSGHRRVAPY
                     GMAGGSPGELGRNRVERADGSTVELAGCDSADVRPGDTLVIETPGGGGYGPP"
     gene            complement(4371..5048)
                     /locus_tag="VF874_10225"
     CDS             complement(4371..5048)
                     /locus_tag="VF874_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX9176431.1"
                     /translation="MAGGELGRQPIPEARAPESEDQTARLGDLIRSALATMSYATAAL
                     FGPDPPMVDSIGAAYEALSTLHHEIEDHAAVLLATRARSTHAELSATLAAVHINAEAA
                     RMGQLAREVADIAKTRRAWASMPAPVLGVLRELSEVCLDLAAKAADVVESHAAVAVAE
                     LDGSEDEMDRLRQLLYWKLLSPSSVVDVGAAIDLTLAARCYERFAEHAVAIAHRGALL
                     AVGAPKS"
     gene            complement(5158..5919)
                     /locus_tag="VF874_10230"
     CDS             complement(5158..5919)
                     /locus_tag="VF874_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX9176432.1"
                     /translation="MSSSEQLQQRAASLLALHRPGDPVVLPTVWDAWSANLAVEAGFA
                     ALTIGSHPVADSVGKPDGEGMTFDDVLARVAQITGAVDLPVSVDVESGYGEPPERLID
                     GLLGVGAVGFNIEDTVHREGKRLRSVEEHAELVGRLRSAADAAGVHVVINARTDLFLR
                     GDGDEADRVDRAIARLSAFAEAGADVLYPVGRHDPDTMRRLTSQLPLPVNAIAVPDQD
                     DPASFGPLAVARISFGPFWQAALGARAREILARWR"
     gene            6055..>6306
                     /locus_tag="VF874_10235"
     CDS             6055..>6306
                     /locus_tag="VF874_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176433.1"
                     /translation="MSAPTRPSQGTGVFSPTRADVLERTLRTDKWWLPTLWTNLGFAA
                     FVIYATVRAFMGHWLQWYWVADYHYLTPFYSPCVSTGCVA"
BASE COUNT         1044 a         2164 c         2082 g         1016 t
ORIGIN      
        1 atccgcgaca ccctgaccgc caacggctgg cggatcgact ttctgggtcc gaccacgtac
       61 ctggcgaaca ccagctcgat ggccggggat ccgaacaatc tgcctgcgca gatgcgccag
      121 cagatgtccc cggagcaagt cgaactgatg aagaaaaccc gcgagcggtt cgccgcgatc
      181 gtgccgctgc tcgacgatga ccgcgtgcac ctcccgttca cggtcgtaca cgcggtacgg
      241 gtggattgac gggtctcttc ggccccgcta cggcgaacga ctggtcgttc aagccggcgg
      301 ccgaggtcgt caaggacggc gaagacgcgg tgatccgcgt cgaactgcca ggcatcgacg
      361 tagagaagga cgtcaacgtc gaggtcgaca agggcgccct cgtcatccac ggggagcgcc
      421 gcgacgagca ctcagaggag aaggacggac gcgccctgcg cgaggtgcgc tacggatcgt
      481 tgcgccggtc gttcgccctg ccgtcgcaca tcacgggcga cgcggtgacg gccagctacg
      541 acgccggcgt cctcactgtc cgggtggccg gcgcctacgc gggcagcgag ccgaagcgca
      601 tcccgatcac caagtaacgg ttgacaattc agtaccggtg aggccgctgg cttcaccggt
      661 cctgtcatgt ctgtcagggt gggccatatc cgccgccgcc gggggtttcg atcaccagtg
      721 tgtcgccggg ccggacgtcg gccgagtcgc acccggccag ctcgacggtg ctgccgtcgg
      781 cgcgttccac gcggttgcgt cccagctccc cgggtgagcc gccggccata ccgtacggcg
      841 cgactcgccg atgcccagac aacgtgctca cggtcatcgg ctcggtgaac tcgatccggc
      901 gcacggcgcc gtcgccgccg tgccaccggc cggcaccgcc gctgtcgcgc cggatggcga
      961 attcccgcag cagcacgggg aaacgccatt cgagcacttc gggatcggta agccgagagt
     1021 tggtcatgtg cgtctgcacg acggacgccc cgtcgaaccc atcaccggcc ccggagcccg
     1081 atccgacggt ttcgtaatac tgatgccgct cgttgccgaa cgtgacgttg ttcatcgtgc
     1141 cggccccttc ggcctgcaca cgcagcgccg cgaacagtgc gccggtgatc gcctgcgagg
     1201 tttcgacgtt gccggcgacg accgcggccg ggtaggtggg cgcgagcatc gactgttctg
     1261 ggatgacgat gtgcagcgga cgcaggcagc cgtcgttgag ggggatgtcg gcggcgacca
     1321 gggtccgaaa cacgtacagc accgcggcgg tcaccactga ggacggtgcg ttgaagttgg
     1381 tgccgagctg gggcgaggtt ccggtgaagt cgatggtcgc gcttcgggtg gcgcggttga
     1441 cggtgatgcg gacgacgatc gaggcgccgg aatccatgcg gtagcggtag tcgccgtcgt
     1501 cgagccggtc gatgacgagg cggaccgctt cctccgcgtt gtcctggacg tggcgcatgt
     1561 atgcctggac gacatcgatg ccgaagtggt cgatcatctt gccgacctcg tcgacgccct
     1621 tctggttggc ggcgatctgc gcgcggagat cggcgaggtt ggtgtcggga ttgcgggatg
     1681 ggtaacgcgc ttcggtgagc aggcgccggg ttgccgcctc tcggaactgt ccgttttccg
     1741 cgagcagcca gtcgtcgaaa agtacgccct cttcatggat ttcgctgctg tcggcgggca
     1801 tggagccggg ggtgatcccg ccgatttcgg cgtgatgccc acgcgaagcg acaaagaaca
     1861 ggattccccc gccagcggcg tcgaagaccg gggtgacgac ggtgatatcc ggcaggtggg
     1921 ttccgccgtg gtacgggtcg ttgaccgcgt acacgtcgcc gggcttcatt tcgccggcgc
     1981 gtctgcggat cacttccttg acagtggtgc ccatcgagcc gagatgcacc gggatatgcg
     2041 gcgcattggc gacgaggtcg ccgtccggat cgaacagcgc acacgaaaag tcaagccgtt
     2101 cacggatatt cactgactgg gcagtggcct ccagccggaa gcccatctgc tcggcgatcg
     2161 acatgaacag gttgttgaag atctccagca gcaccgggtc cgcctcggtg ccggcgtcgg
     2221 gctccgcggg ggccaccact cgctccgcga gcaggtgccc ggaggcggtc atggtcgccc
     2281 gccagccgtc gtcgacgacg gtggtggcgt tggcctctgc gacgatcgcc gggccgacca
     2341 cggtgtcccc ctgccgcatc cgttcgcgat gacacagtgg ggcgtcgcgc cacgatccac
     2401 ctgagtagac ccggacggtt tccgacgcgc cggccgtgtc agcggcccga tccccaagat
     2461 cggacaggtc gggttgctcg gtgagcccga tggcctcgac cgagacggct tcggcgatca
     2521 gcggacggtc catcaggaac gagtaggtcg cgcggtgact ggcttcgaac gctgcggtca
     2581 tcgcatctac ttcggcaagc tcgaccggga ccggagtgtc ggtgccctcg tagcgcagat
     2641 gcacccgccg acccacccgg atccgctcga cccggatccc ctcgtcgagc agctcggcac
     2701 gggcggtctg ctccagggac tccgcgacgg tggtcaacct ttccagcgcg ggggtttcga
     2761 gccgcatctc tacggactgc tcgcgcatcg cggtggtgtc ggccaacccg atcccgagcg
     2821 cggagagcac gccggccatc gggggaacga gcaccgtgcg gatacccagt gcgtccgcta
     2881 cagcgcacgc gtgctgccca ccggcaccgc cgaatgtggt tagcacgtaa cgggttacgt
     2941 cgtgtccctt ttgaacggag atcttcttga ccgcgttggc catgttcgca accgcgatcc
     3001 gcaggaaccc ctcggcgacc tgctcgggcg gacggtcatc accagtccgc gcccggatgt
     3061 ctgcggccag gtcggtgaag cggcgccgca cgatctcgtc atccagcggc tggtcaccgg
     3121 cgggaccgaa cactgctggg aagtgtgcgg cctggatgcg gccgagcatc acgttggcgt
     3181 cggtgactgc aagcggaccg ccgccgcggt agcacgccgg gcccggatcg gccccggccg
     3241 agtccgggcc cacccggtag cgggtgccgt cgaaatgcag aatcgaccca ccgcccgccg
     3301 ccaccgtgtg gatgtccagc atgggagcgc gcagccggac ccccgccacc tgggtggtga
     3361 acacacgctc gtactcgccg ccatagtgcg acacgtcggt ggaggtgccg cccatgtcga
     3421 agccgatgac gtgatcgaac ccggccagcg ccgacatccg caccatgccg acgatgcccc
     3481 cggccgggcc ggacaggatc gcgtccttgc cgcggaaatg tcccgcctcg gccagccccc
     3541 cgttggactg catgaacatc agccggacac catgcatctg atctgctacc tggttgacat
     3601 agcggcgcag aaccggggac aggtaggcgt cgaccacggt ggtgtcccca cgcgggacca
     3661 gtttcatgag tgggctgacc tcggatgaca gcgagatctg cgggaagccg atctgctcgg
     3721 cgagcttccc gatctcccgc tcgtgtgccg ggtgcaggta actgtgcagg cacaccaccg
     3781 cgaccgcacg gatcccgtct gcgtgggccc gccgcagctg ctcgcccagc agctcgagat
     3841 ccggcgcgcg cagcaccgtg ccttcggccg tgatccgctc gtcgacctcg acgacgcgct
     3901 cgtacagcat ctccggcagc acaatgtgcc ggtcaaaaat gcgcgggcga ttctggtagg
     3961 caatgcgcag cgcgtcgccg aaaccgcggg tgataaccag caccgtgcgg tcccccttgc
     4021 gctccagcag cgcattggtc gccacggtgg tgcccatccg gacagcttcg acccgcgcga
     4081 ccggaacggg cactcccacc gcggaagtcc cgagcagcgc acggataccg gccaccgccg
     4141 cgtcgcggta ccgcgaggga ttctccgaca gcagcttgtg agtcagcagg cgcccgtccg
     4201 gccggcgcgc cacgacgtca gtaaacgtgc cgccccggtc gacccagaac tgccaccccg
     4261 caccagccac caaggactcc ctttcacggt tcaccaactc tatccacgag ctgggggacc
     4321 gaagacgggc caccaatctc gccgagttcg cggagatcgc ggtactgccc tcagctcttc
     4381 ggcgcgccca ccgcaagcag cgcaccgcga tgcgcgatcg ccacagcgtg ctcggcaaag
     4441 cgttcgtagc agcgggcagc caaggtcaga tctatcgccg cgccgacatc gaccacactg
     4501 gacggggaca gcagcttcca atacagcagc tgtcgtaatc gatccatttc gtcctcgctc
     4561 ccgtccaact ccgccacggc gacggccgcg tgcgactcca cgacgtcggc cgccttggcg
     4621 gcaaggtcca ggcaaacctc actcagctca cgaagcacgc ccagcactgg agccggcatg
     4681 gacgcccacg cacgccgggt cttggcgata tcggcgacct cccgcgccag ttgtcccatg
     4741 cgcgcggctt cggcgttgat gtgcacagcg gcgagggtcg ccgacagctc cgcatgtgtc
     4801 gaacgggcac gagttgccaa gagcactgct gcatggtcct cgatttcatg atggagcgtg
     4861 gacaacgcct cataggcggc gccgatactg tcgaccatcg gaggatcagg accgaacagg
     4921 gcagccgtcg cgtagctcat cgtagccagc gccgagcgaa tcaagtcgcc cagccgagca
     4981 gtctggtctt cggactctgg ggcacgcgct tccgggatag gttgacgtcc cagttcgccg
     5041 ccggccatga tcggtaacag tacccactgg caagagccca caaagtgttc ggtgccaacg
     5101 gatccgggcc atgccgtgaa tcgcggcgct gtagtcctca ttggtcacgg cgccatgtca
     5161 gcgccagcga gccaggatct cgcgggcccg agcgcccagc gccgcctgcc agaacgggcc
     5221 gaagctgatc cgcgcgacgg ccaacggacc gaacgacgcc gggtcgtcct ggtccggcac
     5281 cgcgatcgcg ttgaccggca gcggcagctg gcttgtcaag cggcgcatgg tgtccgggtc
     5341 gtggcggccg acggggtaga gcacgtcggc gccggcctcg gcaaaggcgc tcagccgcgc
     5401 tatcgcccgg tcgacgcggt cggcttcgtc gccgtcgccg cgcaggaaaa ggtcggtccg
     5461 ggcgttgatc acgacatgca ccccggcggc gtccgcagcg gaccgcagcc ggccgaccag
     5521 ctcggcgtgc tcctcaacag acctcagccg cttgccctcg cggtgcacgg tgtcctcgat
     5581 gttgaacccg accgcgccga caccgagcaa tccgtcgatc aaccgctccg gcggctcgcc
     5641 gtatcccgat tcgacgtcca ccgacaccgg caggtcgacc gcacccgtga tctgtgccac
     5701 gcgggccagc acgtcgtcga acgtcatgcc ttcgccgtcg ggtttgccga cggagtccgc
     5761 aaccgggtgg ctgccgatcg tcagcgccgc gaatcccgct tcgacggcga ggtttgccga
     5821 ccacgcatcc cacaccgtcg gcagcaccac cggatcgccg ggccggtgca gggcgagcag
     5881 cgatgccgcc cgctgttgaa gttgctcgct ggaagacacc ccgtacctcc tgttcaacgg
     5941 acgtgacagg tgtcaccccg gactcgcttc tgtgggtttc accgcgtgtc gtactgtgct
     6001 cttgacacct tcagcccaag gaggtctgtt gtccagcatc aatggtattt cccgatgagc
     6061 gcgcccaccc gaccgagcca aggcaccggg gtcttttccc ccacccgcgc agacgttctc
     6121 gaacgcacgc tgcgcaccga caagtggtgg ctgccgacgc tgtggacaaa tctggggttc
     6181 gccgcgttcg tcatctacgc cacggtgcgt gccttcatgg gccattggct ccagtggtac
     6241 tgggttgccg attaccacta cttgacgccg ttctactcac cgtgcgtgag tacgggatgc
     6301 gtagcc
//
LOCUS       DASWBF010000283        12540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_230964,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000283 DASWBF010000000
VERSION     DASWBF010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230964"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1071..1961
                     /locus_tag="VF874_10240"
     CDS             1071..1961
                     /locus_tag="VF874_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9176434.1"
                     /translation="MRDQPVRFGILVGVDGSTSSTAAVQWAAREAAMRNVGLALVHVA
                     APVAAGSSSIVWTGPNPSAALARQAEHAHQLIDDAVKAANDTAGALQIHRDVIFSSPV
                     PTLVDMSEEAQMLVVGRHGRRLLPHVPLGSTSTAMMHQAHCPVAVIPEDFDGATEVTT
                     APVLVGIDGSPASELATAIAFGEASWRGVDLVALHASCDSDPFGIHELEWAVGEPRAH
                     EALAERLAGWQERYPDVTVRRIVVFDRPARHLLERVEAAQLVVVGSRGRGGFAGLLLG
                     SVSTAVAQAARIPVIVARQG"
     gene            complement(2507..2971)
                     /locus_tag="VF874_10245"
     CDS             complement(2507..2971)
                     /locus_tag="VF874_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176435.1"
                     /translation="MQVPIEHDDVRCYTLYRNAFAEREIACPAMVLRDTVLARWVRKH
                     ALAVDVRSSKELAIAIANGIHPGRIVVHGDNLYDPELRCAANVGVGRVIVDSASQADF
                     LASCSENRTHSVLIRTTDPGLRSSWEVSAFIVGGDGGKPGHAVGCLFCTQYR"
     gene            complement(3628..5169)
                     /locus_tag="VF874_10250"
     CDS             complement(3628..5169)
                     /locus_tag="VF874_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9176436.1"
                     /translation="MTTTETAALPTADELRTRARDALRAIGARIELGEPGQYGIPAST
                     PVTGDVLFTLAESTPDDVYATITEAAQAFTVWRTTPAPVRGALVARLGELLARHKTDL
                     AALVTIDAGKITSEALGEVQEMIDICQFAVGLSRQLYGRTIASERPGHRLMETWHPLG
                     VVGVITAFNFPVAVWSWNAAIAMVCGDTVVWKPSELTPLTALACQALIERAAGDVAAP
                     AALSRVILGGREIGERLVDDPRVALLSATGSVRMGAAVGPRVAQRFGRALLELGGNNA
                     AVITPSADLDLAVRAIVFAAAGTSGQRCTTLRRLIVHRSVVDELVQRIVTAFSQLPIG
                     DPSVQETLVGPLINETSYREMTSALQQARADGGEVSGGERVDLRPNSYYVRPAVVRMP
                     TQTELMHRETFAPILYVLTYDELNEAIALNNAVPQGLSSSIFTLDLREAERFMAANGS
                     DCGITNVNIGTSGAEIGGAFGGEKQTGGGRESGSDAWKAYMRRATNTVNYSAQLPLAQ
                     GIHFG"
     gene            complement(5166..6428)
                     /locus_tag="VF874_10255"
     CDS             complement(5166..6428)
                     /locus_tag="VF874_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9176437.1"
                     /translation="MRHIKLWELRARFAWALSAMYGAEVPAYTTLVEVSSEVNRDYLA
                     AHQDAKRLGSLDRVSAERHGAIRVGTPRELADVAELFAAFGMYPVGFYDLRDAAPPVP
                     VVSTAFRPIDADELARNPFRVFTSMLATDDNRFFGTDLRPRVQRFLAERELFDPALIA
                     EARRIAADGGTTPGRAERFVDAAVAAFALRHEPIDNSWYDELTRVSAVAADVAGLRST
                     HVNHLTPRVLDIDELYRRMNARGIMMIDAIQGPPRWDGPDVLLRQTSFRALAEPRRFR
                     HADGSVSHGTLRVRFGEVEARGVALTPTGRQRYDDAMAAADLAAAAGLPWQEAAESVW
                     PQYFPATDAGLAADELAYYRHSDPSQPIVYEDFLPRSAAGIFRSNLDSDAVAGGESCN
                     TDYDVDWMSGAIGHHIHDPYALYEKAAP"
     gene            6459..6920
                     /locus_tag="VF874_10260"
     CDS             6459..6920
                     /locus_tag="VF874_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9176438.1"
                     /translation="MADAGEQLDDIDRILVRQLLADGRATLAQLASAAGLSVSAVQSR
                     VRRLETRGIVTGYTVRIDHAALGQMLSAFVAITPLDPSQPDDAPARLEHIEAIESCHS
                     VAGEESYILMVRVASPRALEELLQQIRTTANVRTRSTIILQTFYEGRHFIS"
     gene            6973..8319
                     /gene="lat"
                     /locus_tag="VF874_10265"
     CDS             6973..8319
                     /gene="lat"
                     /locus_tag="VF874_10265"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HEX9176439.1"
                     /translation="MTAVLTPSVQNPADELLAPTQVHSVLSRSILVDGLDFVLDLDRS
                     SGSYLVDARSGECYLDMFTFFASAPLGMNHPALVDDYAFRDELAEAAVNKPSNSDVYS
                     VPMARFVETFVRVLGDPALPHLFFVDGGALAVENALKVAFDWKSRHNEAHGIEAALGT
                     RVLHLRGAFHGRSGYTLSLTNTDPIKVARFPKFDWPRIDAPFIRPGVNMDELEVKALQ
                     HARRAFEAHPHDIACFIAEPIQGEGGDRHFRPQFFAAMRELCDEFDALLIFDEVQTGC
                     GLTGTAWAYQQLRVTPDVVAFGKKTQVCGVMAGRRVDEVADNVFAVSSRLNSTWGGNL
                     VDMVRARRLLEVIEADDLFARAVSHGQYLQDRLNELADELAGQVLDVRGRGLMCAFSL
                     PTQAQRDELIRRMWHRRVIMLASGTHGVRFRPPLTVSRGEIDAAVDAVRDSLSRPD"
     gene            complement(8428..8871)
                     /locus_tag="VF874_10270"
     CDS             complement(8428..8871)
                     /locus_tag="VF874_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9176440.1"
                     /translation="MHAGDLVEHFPVVTMESGALETVRLLAEHRLPGIVVTDESRTPR
                     AVLPASQVVRFLVPRYVQDDPSLAGVLNESMADRLAEKLTGKKVRDVLPEHLVEVAPV
                     DADDTILEAAAMMARQHSPLLAVMKGDQPYGVITATQLLAAALPH"
     gene            9199..10575
                     /gene="lysA"
                     /locus_tag="VF874_10275"
     CDS             9199..10575
                     /gene="lysA"
                     /locus_tag="VF874_10275"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9176441.1"
                     /translation="MTLLEMLPSLRRAATPRIDRAIWPLTTSVDEVGRLCVGDVPLTE
                     VAAEYRTPTYVLDEADFRYRMRRYRAALPGVDVVYAGKSLLTVAVARWVADEGLGLDV
                     CSGGELATALAGGVDPARIIMHGNVKTPDELRDAATLEVGRIVVDSPIEIAFLSGRVR
                     RRQQVLVRVTPDIDIRGHRAVSTGVIDQKFGFALAGGHADRAVRRVLDQPWLDLVGLH
                     CHIGSQVTDAALYGEAIRRMIAALAGIRASHGVTLSELNIGGGHGVPYVSGDPELDLD
                     ELAHVIDEAVDAACAAERFPRPRIVIEPGRAISARAGVTLYRVIAVKSQPGGRTFVAV
                     DGGMSDNPRVALYDAKYTVVLANRHPLGPTQPMTVVGRHCECGDEIARDVLLPTDVHP
                     GDLLAVACTGAYHHSMASSYNMIGRPPIVAVHGGRACELVRRETIADLLSRDRELTTP
                     QKTGRAEP"
     gene            10748..12223
                     /locus_tag="VF874_10280"
     CDS             10748..12223
                     /locus_tag="VF874_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176442.1"
                     /translation="MGDCVDASVLVVDHDDEVQRRLQRCVAALQRMVDGGWFVGHEDT
                     IGMEVELDLVDPLGRPRLINDAVLARLGRADMQDELGQFNVELSLAPRRLQGRVLYDS
                     ERDLADVLDGCRARIERLGARLVAVGMLPTVSAEQLTVERISANPRYALLCRRMRAAR
                     HRPFLVRILDGCEPVEFTTDVAAPDAATTSLQLHLRVPPDRFAAYYNAAQMIAGAQVA
                     VAANSPYLLTRQAWQETRLTLLAQLLDSRRPREVRAGAPARVRLGDGWVTGPVELFDE
                     LVRLFPPLFPTLEAEDPDAALEAGCAPGLRELRLHDGTVWRWNRPVYDVQAGCPQLRI
                     ENRVLLSGPTAVDMVANAAFYYGLVRAIVDSDDAPFSQMPFAVAERDLHRAARDGLAA
                     RLCWRGIDHPVNQLTREVLLPAAAAGLEAWGVDADDRDRYLGVIEARVRCGRTGAAWQ
                     TQVVRYLEEHGLGRIAALREMTRRYVEHSHTGAPVHEWPVP"
     gene            12289..>12540
                     /locus_tag="VF874_10285"
     CDS             12289..>12540
                     /locus_tag="VF874_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176443.1"
                     /translation="MSDDRYADLETLDLEHPVWDRCFTVAPLVLIGTIDPGGAANLAP
                     KHMAFPLGWDNYFGFVCTPSHATFRNVERTRSFTVTYPRP"
BASE COUNT         2096 a         4156 c         4118 g         2170 t
ORIGIN      
        1 cagccgggtg ctgcggttcg tgggagattt ggcgttgctg tgcaacgtca tccgagcggg
       61 gtctgggccg ccaagtaacc gcccgggaag ggcaatgccg ctgtgcggca atgcccgata
      121 gccactcaca acaccgacgc aaccacgacg acgtggtcga tgatcgccgc cgatgagcct
      181 gcctcgctgg tgattcccgt cggggtaaag agtgcgtcat cgcgcaacac gttggacctc
      241 aagtacccgt ttgaacgggc cccggccgta tctgctgcca gggccctcaa cgcaagaatt
      301 acgtcctgct gcggactcgt ggaagggctg ctaccgcttc ttcgatggcc ctcacacgaa
      361 ccttgacgat ccccctaacg ggcccactcc attgccccag aggccgcccg gactcgatcg
      421 ggtgctaatg ccgcgtcaag gtttgcgccg acggcgttaa gagcgcatca acagaccgct
      481 cgaattcacg aagtcccggt aggagtggta actagccacg tcgcgaggtt acggtccgac
      541 gcaggagggt agcgagatga cctcgtccga acaagttcgc ctgttggacg gtggcgccga
      601 atctgctgca cccattcccg catcattatc acaatcggcg gttaccgaat tcgtgcgacg
      661 acgttgagtg cggtcatacg tgcatcatcg cagcgtcttc ggcggtgggg ccgcctaacg
      721 ccacagcagc gagccaaccg tcgggcatgt cggactgcgc cggcaatcta taccgcctcc
      781 ctgggggcca cgccccctgg tttggggcga gcgagcaggg cgagcatctg cgttggcgca
      841 gcaaccgctg agatcaccca gcccttccgg atgcgcacac gctctgatcg agcttgcgag
      901 gtcgacatcg tgaacattgt cgaacaattt ccggaggttc cttaccgagc agatctagac
      961 cccggttcgt tctgcgccgg tgttgatctg gccggcgtag cgcttgagca cggttcggtg
     1021 gcgccatatg tcgcacggtg acgtggttcg gtgatcggaa ggagcgcgaa atgcgagacc
     1081 agccggtccg tttcgggatc ttggtgggtg tcgacggatc gacgtcctcg accgcggcgg
     1141 tgcagtgggc cgcacgcgag gcagcgatgc gcaatgtcgg attggccctc gtgcatgttg
     1201 ccgccccggt ggccgccggc tcatcaagta tcgtctggac cggacccaac ccctcggcag
     1261 ctttggcccg gcaagcagaa cacgcccatc agctgatcga cgatgccgta aaggcggcca
     1321 atgacaccgc cggggcactc cagatccaca gggacgtgat cttttcttca cctgtaccca
     1381 cgctcgtcga catgtccgaa gaagcccaga tgctcgtggt gggccgccac ggcaggaggt
     1441 tattgccaca cgtgccgctc ggatcgacca gtaccgcgat gatgcatcaa gcacactgcc
     1501 ccgtcgcggt catccctgaa gacttcgacg gcgcaacgga agtcacgacg gcaccggtgc
     1561 tggttggcat tgatgggtcg ccggcatcag aactggccac cgctatagca ttcggcgagg
     1621 cctcgtggcg cggtgtcgac ctcgtggcct tgcacgcctc gtgcgacagc gatccattcg
     1681 gcattcatga gttggaatgg gccgtgggcg aaccgagggc gcacgaagcc cttgcggagc
     1741 gcctggccgg ctggcaggag cgctaccccg atgtaactgt gcggcgcatc gtcgttttcg
     1801 atcggcccgc gcgccacctg ctcgagcgag ttgaggcagc ccagcttgtc gtggtcggca
     1861 gtcgcgggcg tggtggattc gccggactgc tgcttggttc ggtcagtaca gcagtggcgc
     1921 aggcggcccg catcccggtc atcgtcgccc gccaaggctg atccggacag ctgtcatcag
     1981 ccgggtactc gaccgccaca tgcgggccag cagctcagca gtgcgccgtt cggagttgaa
     2041 ggcgcgacgg gggtatccga tcgccggacc accggagggg acctttgggt ggtcagcgca
     2101 gtgaacgact ccgatcctcc gctcgtcgac gcgtggagct aatcgatcag gctaagggga
     2161 tctgaacgtc ggactcaagc cgcggcctcg ctcgacaggg gtggagtcga gcgagagaag
     2221 tacgcgggga cgcgggaaac gcattgtcgt gcaggcgtcg tcgagcgtcg cgccgatccg
     2281 gtcggcaagg cttctcaacg cagtttcgga gtctctcacg ggaagatcga tatgaagacc
     2341 caactgagtg aggacgattg cgtgttgtcc gcggacccgc gccatttcgg cgatcatgtg
     2401 accgatcgct gccgtgcagc tgacgaatgc gccctcgtgc agaccgattc gggcgtccag
     2461 cccctcaagg ttcagccggc ggtgagccag gaccacttct atcgggtcag cggtattggg
     2521 tgcagaagag gcacccgacg gcgtgccccg gtttgcctcc atcacctccc acgatgaacg
     2581 cagacacctc ccaagatgaa cgcagaccgg ggtcggttgt gcggatgagc acgctgtgtg
     2641 ttcggttctc gctgcacgac gccaggaagt cagcctgtga agcagaatcc acgattaccc
     2701 tgcccacacc gacattggcg gcgcagcgca gttccgggtc atacaagtta tcgccgtgca
     2761 cgacaatcct gccagggtgg atgccattcg cgatggcaat ggcaagctct ttgctgctgc
     2821 gcacgtccac ggcgagggcg tgtttgcgta cccacctcgc cagcacggtg tctcgcagca
     2881 ccattgccgg acaggctatt tccctctcag caaacgcgtt gcggtaaagg gtgtagcagc
     2941 gaacgtcatc atgctcgatg ggcacctgca tcccaggccg ccgtgatccc actgcgtagc
     3001 cccgcgccgc cgcgggccca gccgctgcac cccgacgacc ggggtgagtt tcctgcggcc
     3061 gcggcccctg cgggcgcgcc gtcatcccgg cagcaacgga catcataagg aaggcgacca
     3121 gagtcaccga taccttttct caccggccct ggcgctgatg ccaacggcta tccgctcaag
     3181 ccttgcccta gccacgtcgg tcccggcacg cctcttagcg cccttctgac ggcgctgcag
     3241 cgatgtttaa cgacgccttt atgctcacgc gccggtctca caggtcaccg ggcggtcgaa
     3301 cctggggcgc tccccggagt cgagcccagc gagtgctgga tcggcctcga ccagtgccgg
     3361 ccagcaccgg agccgctcgg cccagtcatc cgacggtgga tcggatcgac tatgacttca
     3421 agcgaattca ccgatatgtc agaaaaagga tgcacagcat cgtccactcc accgagcccc
     3481 accttgcctg acgaaggccg agcagggtcg cgtcgctcct ggccccgcga cttgtcatct
     3541 cgcctccaat tagtgtcgtt ttcactggtg atgcaaccag gccgctgtat ccgctcatca
     3601 gtcgcagcgc agcttatgcc ttccggctca accgaaatgg atgccttgtg ccaacggcag
     3661 ctgggctgaa tagttgacgg tgttggtagc gcgccgcatg taggctttcc atgcgtccga
     3721 accggattcc cggccgccac cggtctgctt ctcgccgccg aacgcccccc cgatctcagc
     3781 gccggaggtt ccgatgttga cgttggtgat tccgcagtcc gatccgttcg ctgccatgaa
     3841 gcgttccgcc tcccgcaggt caagagtgaa gatagacgac gacaatcctt gtggcactgc
     3901 gttattcaat gcgatcgcct catttagctc gtcgtaggta agcacgtaga ggatcggcgc
     3961 gaatgtttcg cggtgcatca gctccgtttg cgtgggcatc ctgaccaccg cgggtcgcac
     4021 gtaatacgag ttcgggcgca ggtcgacacg ctcgccgccg gacacctcgc cgccgtcggc
     4081 gcgcgcctgc tgcagcgcgc tggtcatctc gcgataggaa gtctcgttga tcagcggacc
     4141 gaccaacgtt tcctggaccg atggatcgcc gatcggcaac tgcgaaaagg cggtcacaat
     4201 acgctgcacc agttcgtcga ccaccgatcg gtgaactatc agtcgccgca acgtcgtaca
     4261 gcgctgccca gatgttcctg ctgcggcgaa cacaatcgca cgcaccgcca aatcgaggtc
     4321 ggccgacggc gtgatgaccg cagcgttgtt gccgcccagc tccagcagcg cccgaccgaa
     4381 ccgctgcgcc accctgggcc cgaccgccgc gcccatccgg accgagccgg tcgcgctcaa
     4441 cagcgctacc cgcgggtcat ccaccagccg ctctccgatc tcgcgcccgc cgaggatgac
     4501 ccggctgagc gccgccggcg ccgcgacatc gccggccgcc cgttcgatca gcgcctggca
     4561 ggccagagcg gtcagcggcg tcaactccga gggcttccag accaccgtgt cgccgcacac
     4621 cattgcgatg gcagcgttcc acgaccacac cgccactggg aaattgaacg cggtgatcac
     4681 tccgacgacc ccgagcgggt gccaggtttc catcagccgg tgccccggcc gctccgaggc
     4741 gatagtgcgg ccgtacagct gacgcgagag tccgaccgcg aattggcaga tgtcgatcat
     4801 ctcctgcact tcgccgagcg cttcggaggt gatcttgccg gcgtcaatgg tcaccaaggc
     4861 cgccaggtcg gtcttgtgcc gggcgagcag ctcgccgagt cgcgccacca gcgcaccgcg
     4921 caccggggcg ggggtggtgc gccacaccgt aaacgcctgc gccgcttcgg tgatcgtggc
     4981 atagacatca tcgggtgtgg actcggcgag ggtgaacaag acgtcgccgg tcaccggggt
     5041 gctggctggt atcccatact ggccgggctc gccaagttcg attcgcgccc cgatggcgcg
     5101 cagcgcgtca cgggcgcgcg tccgcaattc gtcggcggtg ggtaatgcag cggtctcggt
     5161 ggtggtcatg gtgctgcctt ctcgtagagc gcgtaggggt cgtgaatatg gtgcccgatc
     5221 gcaccgctca tccagtcgac gtcgtagtcg gtgttgcagg attcgccgcc ggcgacagcg
     5281 tcagagtcca ggtttgagcg gaaaatcccg gcggccgatc gcggcaagaa atcctcgtac
     5341 acgatcggct gcgaggggtc gctgtggcgg tagtacgcga gctcgtcagc ggccagcccg
     5401 gcgtcggtag cgggaaagta ttgcggccac accgattccg ccgcctcttg ccacggcaga
     5461 ccggcggcgg ccgccaggtc ggccgccgcc atcgcgtcgt cgtaacgttg gcggcccgtc
     5521 ggggtcagcg cgactccgcg ggcttccacc tcgccgaacc ggacccgcag cgttccgtgg
     5581 ctcaccgagc cgtccgcgtg ccggaaccgc cgcggttcgg ccagtgcgcg aaacgacgtc
     5641 tgccgcaaca acacatcagg gccgtcccag cgcggcggac cctggatcgc gtcgatcatc
     5701 atgattccgc gtgcgttcat ccgccggtac agctcgtcga tgtcgagaac ccgtggcgtc
     5761 aagtggttga cgtgggtgga ccgcagcccc gcaacgtcgg cggccaccgc cgacacccgc
     5821 gtcagctcgt cgtaccagga gttgtcgatc ggctcgtggc gcagcgcgaa cgctgccacg
     5881 gccgcgtcaa cgaaccgctc ggcacgccca ggggtggtgc ccccgtcggc ggcgatgcgc
     5941 cgggcctccg caatcagcgc gggatcgaac agttcgcgtt cggcgaggaa ccgctgcacc
     6001 cgggggcgca ggtcggtccc gaaaaaccgg ttgtcgtcgg tggccagcat cgaggtgaat
     6061 acgcgaaacg ggttgcgcgc caactcatca gcgtcgatcg gacggaacgc cgtggacacc
     6121 accggcaccg gcggagccgc gtcgcgcaga tcatagaaac ccaccggata catgccgaag
     6181 gcggcgaaca gctcggcgac atcggcaagt tcacgcgggg tgccgacgcg gatcgcgccg
     6241 tggcgttcgg cgctcacccg gtccagcgac ccgagccgtt tggcatcctg atgagcggcg
     6301 agataatcgc ggttcacctc cgaactgacc tcgaccaggg tggtgtaggc cggcacctcg
     6361 gcgccgtaca ttgcggacag ggcccatgcg aatcgcgcgc gcagctccca cagtttgatg
     6421 tgcctcatcc gacacccgcg cccagataca gtgcggccat ggctgatgcc ggtgaacagc
     6481 tcgatgacat cgaccgcatc ctcgtgcgcc agctgttggc ggacggccgg gccacgctgg
     6541 cacagctggc ctccgccgcg ggcctgtcgg tgtcggcagt ccagtcacgg gtgcggcggc
     6601 tggaaacccg gggcatcgtc accgggtaca ccgtgcgcat cgaccacgcg gcgctggggc
     6661 agatgttgtc ggcgttcgtc gccatcactc ctctcgatcc ttcccaaccc gatgatgcgc
     6721 ccgcccgcct tgaacacatt gaggccattg agtcctgcca ctcggtggcg ggcgaggaaa
     6781 gctacatcct gatggtgcgc gtggcctccc cgcgggcact cgaagagctg ctgcaacaga
     6841 tccgcacgac ggccaatgtg cggacgcgaa gcaccatcat cttacaaaca ttttacgagg
     6901 gacggcactt tatatcgtag ttgttactgt tttcacgaat ctttatgaaa aattatcggg
     6961 taccatccca gtatgaccgc cgttcttaca ccctccgtgc agaacccggc cgacgagctg
     7021 cttgcgccca cccaggtgca ttcggtgctg agccggagca tcctggtcga cggcctcgac
     7081 tttgtactcg acctcgaccg ttcctcgggg tcgtatctgg tcgacgcacg cagcggcgag
     7141 tgctatctcg acatgttcac cttcttcgct tccgcgccgc tgggcatgaa ccacccggcg
     7201 ctggtggacg attacgcctt ccgcgacgaa ctggccgaag ccgcggtcaa caagccgagc
     7261 aactccgacg tttacagcgt gccgatggcc cggttcgtcg agaccttcgt gcgcgttctc
     7321 ggcgatccag cactgccgca cctgttcttc gtcgacggcg gcgcactagc ggtcgagaac
     7381 gccctcaagg tggccttcga ctggaagagc cgccataacg aggcgcacgg catcgaagcg
     7441 gcgctgggca cccgggtgct gcacctgcgc ggagcgttcc acggtcgcag cgggtacacg
     7501 ctgtcgctga ccaacaccga cccgatcaag gtggccaggt tcccgaaatt cgactggccg
     7561 cgcatcgacg cacccttcat ccgccccggc gtcaacatgg acgaactgga ggtaaaggca
     7621 ctgcaacacg ctcgccgcgc gttcgaggcc cacccccacg acatcgcctg tttcatcgcc
     7681 gagccgatcc agggcgaggg aggtgatcgt catttccggc cgcagttttt cgccgcaatg
     7741 cgtgagctat gtgacgagtt tgacgcactg ctgattttcg acgaggtgca gaccggctgc
     7801 ggattgaccg gcaccgcgtg ggcctatcag cagctcaggg tgacacccga tgtcgtcgcg
     7861 ttcggcaaga agacacaggt gtgcggcgta atggctggcc gccgggtgga tgaggtcgct
     7921 gacaacgtgt tcgccgtcag ctctcggctc aactccacct ggggcggcaa cctggtggac
     7981 atggtccgtg cacggcggct tctggaggtg atcgaggccg acgatctgtt cgcgcgcgcc
     8041 gtttcacacg gccagtatct gcaggatcgg ctcaacgagt tggccgacga gctagctggc
     8101 caggtgctcg acgtgcgcgg ccgcggactg atgtgcgcgt tcagtctgcc gacgcaggct
     8161 cagcgcgacg agctaatccg ccggatgtgg catcggcggg tcatcatgct ggccagcggc
     8221 acccacggcg tgcggttccg gccgccgctg accgtctcac gcggcgaaat cgacgcggcc
     8281 gtggacgccg tgcgcgactc gctgagccgc cccgactagc gaccggtggt gcccgatcag
     8341 ccggaagccg gtgtgaccca tgacgacaga atcacatcgc aacccaaaca acgatgtggc
     8401 tggcttgttc gggttcggcc acgaacctca gtgcggtaag gccgcggcca gcaattgcgt
     8461 tgcggtgatc accccatacg gttgatcgcc cttcatcacg gccaacagcg ggctgtgctg
     8521 gcgcgccatc attgcggccg cttccaggat cgtgtcgtcg gcgtcgacgg gtgccacctc
     8581 gacgaggtgc tcgggcagaa cgtcgcgcac cttcttgccc gtcagcttct cggctaatcg
     8641 gtcagccata gattcgttga gcacgccggc cagcgacggg tcgtcctgca cgtagcgggg
     8701 caccaggaat cgcaccacct gcgacgccgg aagcaccgcc cgcggcgtgc gcgactcgtc
     8761 ggtgacgacg atgccgggga gccgatgctc ggccaataac cggacagtct ccagtgcgcc
     8821 ggattccatc gtcactaccg ggaaatgttc cacgaggtcg cccgcgtgca caccagcaca
     8881 ttaggcacgc ggcggcggcg ctaccgagcc gtgaggccgt caaaatttgg cttgcaggcg
     8941 tcaggggttc gttaaggttt ctcggatcgc catcggccgc gcgcacggtg ggtccatgac
     9001 gcttctggag ctgtcgccgt cgctgcgttc agcggcgact catcgacagg aggcaggagg
     9061 gttagaagtc gcgtcccacc gctaggcggc gacatctctg ttgtcccgag gagggatgtc
     9121 gtcaagattg cccctggtgc cgtcaagagg ctgtcaaggg ttgtcgcagt gatgcccaac
     9181 cgggcggaac gtggatctat gacattgttg gagatgttgc cgtccctgcg ccgggcggcc
     9241 actccccgca tagaccgggc gatctggccg ctgacaacca gtgtcgacga agtggggcgg
     9301 ctctgtgtgg gcgatgtgcc gttgaccgag gtcgccgctg agtatcgcac ccccacttat
     9361 gtccttgacg aggccgattt ccggtatcgc atgcggcgct atcgcgcggc gctgcccggg
     9421 gttgacgtgg tctatgccgg gaagtcgctg ctgactgtcg cggtggcacg ctgggttgcc
     9481 gacgaggggc tgggtctgga cgtgtgctcg ggcggcgaac tggccaccgc ccttgccggc
     9541 ggcgtggacc cggcgcggat catcatgcac ggcaacgtga aaacgcccga cgagttgcgt
     9601 gacgccgcca ccctcgaagt gggccggatt gtggtggact cgccgatcga gatcgccttc
     9661 ctgtccggtc gggtgcgccg ccgccagcag gtgctggtgc gggtaacccc cgacatcgac
     9721 atccgtgggc accgcgcggt gagcaccggg gtgatcgatc agaagttcgg cttcgcgttg
     9781 gccggcgggc atgccgaccg ggcggtgcgg cgcgtgctgg atcagccgtg gctggatctg
     9841 gtcggattgc attgccacat cggctcgcaa gtcaccgatg ccgcgctcta cggcgaggcc
     9901 atccggcgga tgatcgccgc gctggccggt atccgggcaa gccacggcgt caccctcagc
     9961 gagctgaata tcggtggcgg acacggcgtt ccctacgttt caggtgatcc cgagctggac
    10021 ctggacgagc tggcacatgt catcgacgag gcggttgacg ccgcctgcgc cgctgaacgg
    10081 ttcccgcgcc cgaggatcgt gattgagccg gggcgcgcga tcagtgcacg cgccggcgtc
    10141 accctgtacc gagtaattgc ggtgaaatcg caaccgggcg gtcgaacctt cgtcgccgtg
    10201 gacggcggca tgagcgacaa ccctcgggtg gctctctatg acgccaaata cacggtggtg
    10261 ctggctaatc gccacccgct ggggccgacc caaccgatga ccgtggtcgg gcgacattgc
    10321 gaatgcggcg acgaaatcgc ccgcgacgtg ctcctgccga ccgatgtcca ccccggcgac
    10381 ctgctcgcgg tcgcgtgcac cggcgcctat caccacagca tggcctcgag ctacaacatg
    10441 atagggcggc caccgattgt cgctgtccac ggcggacggg cctgcgaact ggtgcgccgc
    10501 gagacgatcg ccgacctact ctcccgcgac cgtgagttga ccaccccgca aaagacgggc
    10561 cgggcagagc cctgaccagt ggcagcacct gccacggcgg cgtgaaacac cggcttcacc
    10621 cagcctcgcg gtcgatgacc agccggcgta aaggtcatct caaggttgtg atggtctgcg
    10681 ggcaggctcg gctgcgccgt gctggagtag aggttcggtg tccagcagtt ctgcgaggag
    10741 gtgcgccgtg ggtgactgcg tcgacgcgtc ggtcctggtt gttgaccacg acgacgaggt
    10801 gcagcgcagg ctccagcgct gtgttgcggc gttgcagcgc atggttgatg gcggctggtt
    10861 cgtcggccat gaggacacca tcggtatgga ggtcgagctc gatctcgtgg acccgttggg
    10921 caggccgcgg ctgatcaatg acgcggtcct ggcacgcctg ggccgggcgg acatgcagga
    10981 cgagctcggt cagttcaacg tggagctgag cctggctcct cgccggctgc agggccgcgt
    11041 gctgtatgac agcgaacgcg acctcgccga cgtgctcgac gggtgcaggg cgcggatcga
    11101 gcggctcggc gctcggctgg tcgccgtcgg catgctgccc accgtcagcg ccgagcagct
    11161 gaccgtcgag cggatctccg ccaacccgcg ctacgcgctg ctgtgccgcc gcatgcgtgc
    11221 cgctcgccac aggccgttcc tggtgcgcat cctcgacggg tgtgagccgg tggagttcac
    11281 caccgacgtg gccgctccgg atgccgccac gaccagcctg cagctgcacc tgcgggtgcc
    11341 gccggatcgg ttcgccgcct actacaacgc cgcccaaatg atcgccgggg cgcaggtggc
    11401 ggtggccgcg aactcgccgt acctgctcac ccgccaggcc tggcaggaaa ctcgcctcac
    11461 tttgttagcg cagctgctcg acagccgccg accgagggag gtccgggccg gcgcaccggc
    11521 gcgcgtccgg ctgggggacg gctgggtcac cggtcccgtg gagctcttcg acgagctggt
    11581 gcggctgttt ccgccgctct ttccgacgct ggaggccgag gatcccgatg ccgccctgga
    11641 agcggggtgt gctccgggcc tgcgtgagct tcgcctgcac gacggcacgg tgtggcgctg
    11701 gaaccgtccg gtctacgacg tccaagcggg gtgccctcag ttgcgcatcg agaaccgggt
    11761 actgctcagc ggaccgaccg ctgtcgacat ggtcgccaac gccgccttct actacggact
    11821 ggtccgcgcg atcgtcgaca gcgacgatgc cccgttcagt cagatgccct tcgcggtggc
    11881 cgagcgggac ctgcaccgcg cggcacgcga cgggctggcg gcgcggctgt gctggagggg
    11941 catcgaccac ccggtcaatc agctcacccg tgaggtcctg ctgccggccg ccgcggccgg
    12001 gctcgaggcc tggggcgtgg acgcggacga ccgcgaccgc tacctcggtg tcatcgaagc
    12061 gcgggtccgg tgcggccgta ccggcgccgc ctggcagacg caggtggtgc gctacctcga
    12121 ggagcacggg ctggggcgta tcgcggcgct gcgtgagatg acccggcggt atgtcgagca
    12181 ctcgcacacc ggcgccccag ttcacgaatg gcccgtgcca tgaccggggg ctacgataga
    12241 cggcgccggc gccagcgtgg cggccagcgg gccgcgagga gttgcgcaat gtctgacgat
    12301 cgctacgccg acctcgagac cctcgatctc gagcaccccg tctgggaccg ctgcttcacg
    12361 gtcgccccgc tcgtgctcat cggcacgatc gaccccggag gcgcggccaa cctcgccccg
    12421 aagcacatgg cgtttccact cggctgggac aactacttcg gtttcgtatg cacaccgagc
    12481 catgccacgt ttcgcaacgt cgagcgcacg cgaagcttca ccgtcaccta tccgcgccca
//
LOCUS       DASWBF010000284         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_421373,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000284 DASWBF010000000
VERSION     DASWBF010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421373"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..522)
                     /locus_tag="VF874_10290"
     CDS             complement(172..522)
                     /locus_tag="VF874_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9176444.1"
                     /translation="MALNVEMVTFDCSDPAPLAAWWAEQFDGKTQELIPGEFVVVIRS
                     EGPRLGFQKVPDPTPGKNRIHLDFSAADVDGEVARLVKAGATESGRHSVGENFRWVVL
                     ADPEGNAFCVAGSS"
     gene            complement(540..>1525)
                     /locus_tag="VF874_10295"
     CDS             complement(540..>1525)
                     /locus_tag="VF874_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HEX9176445.1"
                     /translation="VAGWQVVALQPYGGAWLNSWLDRDLGISGRLSVREGATTRQHLV
                     RIEEPLLRVPQLAIHLAEDRKSVKLDPQRHVNAVWGVGGGARSFLAFVAERAGVDADA
                     VLGADLMTHDVQPSRVIGANGKLVSAPRLDNQATCYAGLEAFLTAEPRGYVPVLALFD
                     HEEVGSTSDHGAQSNLLTTVLERIVLAEGGTREDYLRRLPNSVVASGDMAHATHPNYP
                     ERHEPGHLIEVNAGPVLKVQPNLRYATDGRSAAVFALACAQAGVPLQRYEHRADLQCG
                     STIGPMTSARTGIPTVDVGAAQLAMHSARELMGADDVAAYSAALHAFLAPD"
BASE COUNT          245 a          547 c          500 g          233 t
ORIGIN      
        1 tcgacggtat cgagcgaggg cgtggtctgc ggcggcgtcg gcgatgagcc gcttgcgcga
       61 agagaatcag actcggcgag cggtgacgtc acccagacag cttaagttcc cggcccgccg
      121 cgctcccgtc cgccacgcgc cgggggctca cctggcgccg cagtgtcgcg ctcagctact
      181 gccggccacg cagaacgcgt tgccctcggg atcggccagc accacccagc ggaagttctc
      241 gccgaccgaa tgccggccgg actcggtggc gcctgctttg acgagccgcg ccacttcgcc
      301 gtcgacgtcg gcggcggaga agtccagatg gatgcggttc ttgcccggtg tcgggtcggg
      361 aaccttctgg aaacccagcc gcggaccttc cgagcggatg acgacgacga actcgccggg
      421 tatcagctcc tgcgttttcc cgtcgaactg ttcggcccac cacgccgcga gcggcgcggg
      481 atcggagcag tcgaaggtga ccatctcgac gttgagtgcc atacgtcgag tctacgagct
      541 cagtcgggag ccagaaaggc gtgcagcgcg gccgaatacg cggccacgtc atcggcgccc
      601 atcaactccc gggcggagtg catcgccagc tgtgcggcac cgacgtcgac ggtcggaatc
      661 cccgtgcgcg ccgacgtcat cggaccgatc gtcgacccgc actgaaggtc ggcgcggtgc
      721 tcgtagcgct gcagcggcac ccccgcctgc gcgcaggcca gcgcgaacac cgcagcagag
      781 cggccgtcgg tggcgtagcg caggttcggc tgaaccttga gcaccgggcc ggcgttcact
      841 tcgatcagat gccccggctc gtgacgctcc gggtagttcg gatgcgtcgc gtgggccata
      901 tcgcccgagg ccaccaccga gttcggtaac cgccgcaggt agtcttcgcg agttcctccc
      961 tctgccaaca caattcgttc cagtacggtg gtgagcagat tcgactgcgc gccgtggtcg
     1021 gacgtcgagc ccacttcctc gtggtcgaac agcgccaata cgggtacata cccgcgcggc
     1081 tcggctgtga ggaacgcctc caggccggca tagcaggtgg cctggttgtc cagccgcgga
     1141 gcgctgacca acttgccgtt cgcgccgatc acccgcgacg gttgcacgtc atgggtcatc
     1201 aggtcggcgc ctagcaccgc atccgcgtcc actccggccc gctcggccac gaaggccaga
     1261 aacgaacgag cgccgccgcc gacgccccac accgcgttca catgccgctg cgggtcgagc
     1321 ttcaccgact tgcggtcctc ggccaggtga atggccagct gcggcacccg cagcagcggc
     1381 tcctcgatgc gcaccagatg ctggcgtgtg gtcgcgccct cgcgcaccga cagcctgccg
     1441 ctgatcccca ggtcgcgatc cagccacgag ttcagccacg cgccgccgta cggctgcagc
     1501 gcgaccacct gccatccggc gacga
//
LOCUS       DASWBF010000285        11397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_29951, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000285 DASWBF010000000
VERSION     DASWBF010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29951"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VF874_10300"
     CDS             <1..1459
                     /locus_tag="VF874_10300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176446.1"
                     /translation="EIFGFYGLAYWYAEQVTILGLGPIWQSDNIKAKERAAEQLEAGG
                     VMAFGLSEREHGADIYNTDLLLTAAEEEGVVFRASGEKYYIGNGNVAGMVSVFSRRTD
                     IDGADGYVWFVADSGHENYQLIDNVVHGQMYVSNFRLNDYPVREEDILCTGPDAFSVA
                     LNTVNVGKFNLCTASIGMCEHAFYEAITHANNRILYGNPVTDFPHVRASFVDAYARLI
                     AMKLFSDRAIDYFRSASRDDRRYLLFNPVTKSKVTSEGETVVTLLWDVLAAKGFEKNT
                     YFSEVARLIGALPRLEGTVHVNVAQILKFMPNYMFNPADYPEIGTRNDPADDVFFWAQ
                     GPAGGASKVQFADWTAVYEKSLDIPNVARFYEQACALKELLTTAAPGAEQMRDLDFLL
                     VLGHLFTLVVYGQLILEQAALIDLDRDLVDQIFDVQIRDFSAYAVALHGKPSSTPAQQ
                     EWALSAVRKPVTDGNRFGRVWEQVKADDGAYQMRP"
     gene            complement(1489..1965)
                     /locus_tag="VF874_10305"
     CDS             complement(1489..1965)
                     /locus_tag="VF874_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX9176447.1"
                     /translation="MNTISGLPAHVLLVHAMVVLAPLTALLEILCGIWPAARRRLVWL
                     VLMLALVTAGLTPLTTEAGEWLFDRARQPSEILRTHAERGNWMIYFSVALVVVAIVLA
                     VLHVAEGRSEKRRVGANVLVAILALAVGISTLVTVYQIGDSGARAVWGGQVTQADE"
     gene            complement(2039..2773)
                     /locus_tag="VF874_10310"
     CDS             complement(2039..2773)
                     /locus_tag="VF874_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEX9176448.1"
                     /translation="MTALPESIPGYAMFARYAFPPNELGYCGPPDASVLLRGEDPVEV
                     AAHAREFDGAWPYLQAIADAAGIADPLNDEVVRSYWVGGPLLDRVDPSDLLTRLRRAF
                     AGQVTGLLGDLQSSAGVSAHHSFHVFVVYPWVRFLGGDPTTPVKVMQDCRIRWGTVDY
                     VDAEYAVLVSRPLMFDNDALRLGDPAPEKVRWSKDGVSLAPPPAVGSTVSAHWDWVCA
                     TITDAERDALAAGTQATLDLVNAARA"
     gene            complement(2774..3814)
                     /gene="hypE"
                     /locus_tag="VF874_10315"
     CDS             complement(2774..3814)
                     /gene="hypE"
                     /locus_tag="VF874_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX9176449.1"
                     /translation="MDSWVCPAPLRDAPNIVMGHGGGGAMSAELIEHLFLPAFGPAAD
                     AGMGDSAVMDVGGVRLAFSTDSFVVKPLVFPGGSIGDLAVNGTVNDLAMAGAQPLMMS
                     TAFILEEGTRLDEIGRVAQAVGTAALAAGVKLVTGDTKVVDSGHGDGVFINTAGVGLV
                     DERADIRPQRARPGDVVIVSGDIGVHGVAVMSCREGLEFGTTVASDTAPLHRLVAAMI
                     DTGADIHVLRDPTRGGVAATLNEIAKTAKVGVSLVERSLPVPAEVRDACGLLGLDPLY
                     VANEGKLIAFVPAEDVDKVLAAMREHPLGVRAAAVGTCVAEHAGMVVARTALGGTRVV
                     DMPIGEQLPRIC"
     gene            complement(3900..5000)
                     /gene="hypD"
                     /locus_tag="VF874_10320"
     CDS             complement(3900..5000)
                     /gene="hypD"
                     /locus_tag="VF874_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX9176450.1"
                     /translation="MKYLDEFSDPELAGNLVEQIKAATTQRWAIMEVCGGQTHSIIRH
                     GIDQLLPEQIEMIHGPGCPVCVTPLEIIDKALEIASRPDVIFCSFGDMLRVPGSEKDL
                     FRIKSEGGDVRVVYSPLDALTIAVDNPDKQVVFFGIGFETTAPANAMTVFEAKRRGIE
                     NFSLLVSHVLVPPAIAAIMDSPTCRVQAFLAAGHVCCVMGTDEYPPLTEKYKIPIVVT
                     GFEPLDILEGIRRTVIQLESGRHELENAYPRAVQAAGNSPAKAMLQDVFEVTDRAWRG
                     IGMIPQSGWRLSPAYREFDAEYRFAVTDIHTEESSVCRSGEVLQGLIKPHQCAAFGKQ
                     CTPRNPLGATMVSSEGACAAYYLYRRLEVTHA"
     gene            complement(4997..5314)
                     /locus_tag="VF874_10325"
     CDS             complement(4997..5314)
                     /locus_tag="VF874_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX9176451.1"
                     /translation="MCLAVPGKILSLEDRDGTLMSVVDFGGIKKDVCLQYIPDAQVGE
                     YVIVHVGFAIQRLDEESAMRTLAEFEHLGVMKEEFGDAFELAARQAGMPEPDTNGNGE
                     VKS"
     gene            complement(5364..7655)
                     /gene="hypF"
                     /locus_tag="VF874_10330"
     CDS             complement(5364..7655)
                     /gene="hypF"
                     /locus_tag="VF874_10330"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEX9176452.1"
                     /translation="MTDRQRLRVCVRGVVQGVGFRPFVYTTSAALGLSGCVRNDSSGA
                     IIEVEGDAAAIDEFLHRLRDQPPPLAIIESIATEDIPVLGGTGFTIADTSRSGTGRTL
                     ASPDVAMCADCAAELRDPGNRRYRHPFVNCTNCGPRFTIIGSLPYDRATTTMAAFEMC
                     ADCAREYADPADRRFHAQPVCCPACGPTLSYVTPGRDTVRRESALRAARTLLRDGGVL
                     AVKGIGGYHLACDVRNDAAVAELRRRKRRGDKPFAVMVPDLATARRIAEVDEPAERVL
                     CGPQRPIVLLPRLAHSGVADSVAPRNPDLGIMMTYTPLHVLLFGLQDDEPGPDVLVMT
                     SGNLGGEPICYDDDVVARLADLADGWLMHDRTILVPCDDSVVRVLDGTELPIRRSRGY
                     APLPVALPVPLPPTLAVGADLKNAMAVADGRYAWLSQHIGDMDDVATLSAFDSAQAHL
                     RALTGVDPAVLVADAHPLYRSTGWAHRNADGRPVRTVQHHHAHIAAVMAEHGYDGSEQ
                     VIGFAFDGTGYGTDAAVWGGEVLLANYKGFRRLAQLKYVPLAGGDVSVLRPYRMALAH
                     LWVAGIPWDGDLAPVAACPPAEQKVLAHQLDTGWGCVPTSSMGRLFDAVSSLAGVRHT
                     VDYEAEAAIELEGLARETLCGAAEYAFVLDDSAEPAIIDPAPVLAAIVRDYRAGVQPG
                     VIGVRFHRAVAELIGRLAEDVREATSHNTVALSGGVFQNALLLQLSLTGLRRCGFEVI
                     THRRVPPNDGGIALGQLLAGNSV"
     gene            complement(7652..7900)
                     /locus_tag="VF874_10335"
     CDS             complement(7652..7900)
                     /locus_tag="VF874_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX9176453.1"
                     /translation="MCLGIPGQVVRMLEGYGGQLALVDVAGEHRKVSIGMLPEETFGP
                     GDWVIIHMGFVVEKTDRAGADAAMAGLKLIGRDPEASP"
     gene            complement(7902..8408)
                     /locus_tag="VF874_10340"
     CDS             complement(7902..8408)
                     /locus_tag="VF874_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX9176454.1"
                     /translation="MYDRSDITSRILVAGIGNIFLGDDGFGPEVIKRAGQRLAGTDAR
                     VEDYGIRGMHLAYDLLDDWDALVLVDAVPSRGSPGTLHMFEADHESIVTTPVLDAHSM
                     DPAAVFASLAALGGTPPYTVVVGCEVENVDEGIGLSPSVDAAVDGAVGAVAEVLSTLS
                     ARAATGGS"
     gene            complement(8534..9904)
                     /locus_tag="VF874_10345"
     CDS             complement(8534..9904)
                     /locus_tag="VF874_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176455.1"
                     /translation="MSGGWDRVRTIADAVLYEGYLLYPYRANSRKNQSRWQFGVLGPQ
                     RAEERGIGENDTLSAQVLLDGGTASLSVVVRFLQLQRRTAERDTGDGLFETVDELTAD
                     GQTWLTWDEAVERELSFGPFPVTDLEAGQKIPLTVAGGTDTEAVDGGRLVRSRRELTG
                     ELTLAAELDGNLGLVSIQVRNTGAPPGNKDNAIAASFIGTHLIAEAIDGEFVSLLDPP
                     PLAGDAVARCRQHRCFPVLAGPPGDHSLMLASPIILYDHPEIAEQSEGALYDSTEIDE
                     ILTLRVMTMTDEEKAQARATDPLAAQIIDRCDSMSPEAMLNLHGVLRNPHAREESSSP
                     SALRASGSSLIPEIPDGVDWWDPLADTAVQPEIDAVLVNGVRVSRGSRVRLRPSRRAD
                     AQDIFFSGKTAHVTSVHEDVDGNQHVGVVVDDDPAADLHDWYGRYLYFAPDEVEPLTE
                     RSSQWM"
     gene            complement(9901..10545)
                     /locus_tag="VF874_10350"
     CDS             complement(9901..10545)
                     /locus_tag="VF874_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HEX9176456.1"
                     /translation="MTEVTYAVVDVSPQPYAVTPVLAAQVGVAAVGDDPVHAIALRCQ
                     VRIDPLRRRYSDEEAAGLLDLFGPRERWSTTQRTFVWLHCAVMVPGFSGATQVEMPLE
                     CTYDFEVTAAKYMHALRDGTIPLQFLFSGTVFTQGERGFAVQQVPWDREDRYDLPVSV
                     WRSLMDQHYPNTGWVRLNRDTLDALAAYKSERGMLGFDDAVTSLLETSSQEVQP"
     gene            complement(10577..11209)
                     /locus_tag="VF874_10355"
     CDS             complement(10577..11209)
                     /locus_tag="VF874_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HEX9176457.1"
                     /translation="MTGAYDVLARIRANRNAPQPAGERCEMCTEPIADEHQHVVNVEA
                     RQLMCVCRGCYLLFTDTNAELRYRAIPDRCLTFPDFALDRREWEALQIPVGLAFFFRN
                     SALGRTVAFYPGPAGATESELDLAAWNNIRESDPRVDMLADDTEALIVRVPDEEGGAP
                     ESFLVPIDACYEFVGRLRLLWHGFDGGQQVREFISEFFEQVAARSKVVPR"
     gene            complement(11206..>11397)
                     /locus_tag="VF874_10360"
     CDS             complement(11206..>11397)
                     /locus_tag="VF874_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176458.1"
                     /translation="ALHKMMGTNAPVLRCPRCRAHFDVVHAGRCVDGNGHSDAHLEPI
                     PLLERDGVLSMALARAVAT"
BASE COUNT         1896 a         3907 c         3750 g         1844 t
ORIGIN      
        1 cgagatcttc ggcttctacg ggctggcgta ctggtatgcc gagcaggtca cgatcctcgg
       61 cctcgggccg atctggcaga gcgacaacat caaagccaag gagcgcgcgg ccgagcaact
      121 cgaggcgggc ggcgtgatgg cgttcgggct ctccgagcgc gagcacggcg cggacatcta
      181 caacaccgac ctgcttctca ccgctgcgga ggaagagggc gtcgtttttc gggcgtcagg
      241 ggagaagtac tacatcggca acggcaacgt cgccggcatg gtgtcggtgt tctcgcgccg
      301 taccgacatc gacggcgccg acggatacgt ctggttcgtc gccgacagcg gccacgagaa
      361 ctaccagctg atcgacaacg tggtgcacgg ccagatgtat gtcagcaact tccggctcaa
      421 cgattacccg gtgcgcgagg aagacatcct gtgtaccggg cctgacgcgt tctcggttgc
      481 gctcaacacc gtcaacgtcg gcaagttcaa cctgtgcacc gcctcgatcg ggatgtgcga
      541 gcacgccttc tacgaggcga tcacgcacgc gaacaaccgc attctctacg gcaacccggt
      601 caccgacttc ccgcatgtgc gcgcgagttt cgtcgacgcg tacgcccggt tgatcgcgat
      661 gaagctgttc agcgaccgag cgatcgacta cttccgcagc gccagccgcg acgaccgtcg
      721 ctacctgctg ttcaatcccg tgaccaaatc caaggtcacc tccgagggcg aaaccgtggt
      781 cacgctgcta tgggatgtgc tcgccgccaa aggcttcgag aagaacacct acttcagcga
      841 ggtcgcccgg ctgatcggtg cgttgcctcg gctggagggc accgtgcacg tcaacgtcgc
      901 gcagatcctg aaattcatgc cgaactacat gttcaaccct gccgactacc cggagatcgg
      961 aacccgcaat gacccggcgg acgacgtgtt cttctgggcg caaggtccgg ctgggggtgc
     1021 atccaaggtg cagttcgcgg attggactgc ggtctacgag aaatccctgg acataccgaa
     1081 cgtcgcccgc ttctatgagc aggcttgcgc actgaaggag ctgctgacca cggctgcacc
     1141 gggcgccgag cagatgcgtg acctcgactt ccttctcgtc ctagggcatc tgttcacgct
     1201 tgtcgtctat ggccagttga ttctcgagca agccgcgctg atcgatctgg accgtgacct
     1261 ggtagaccag atcttcgacg tccagatacg cgacttctcc gcatatgcag tcgcgttgca
     1321 cggcaagccg agttctactc cggcgcaaca ggaatgggcg ctgtcagccg tgcgcaagcc
     1381 ggtcaccgat gggaaccgct tcggccgggt ctgggagcag gtcaaggccg acgacggcgc
     1441 ctaccagatg cgtccgtagc accgcccctg atgccgcgca acagggcgct actcgtcggc
     1501 ttgcgtgacc tggccgcccc acacggcgcg tgcgccggag tcaccgatct ggtacacggt
     1561 gaccaatgtc gagataccga cggccagcgc gaggatggcg acgagcacgt tggcgccgac
     1621 ccggcgcttc tccgaccggc cctcggcgac atgcagcacc gccagtacga tcgcgacgac
     1681 caccagcgcg acggagaagt agatcatcca gttgccgcgt tcggcatgcg tgcgcaggat
     1741 ctcgctcggt tggcgcgcgc ggtcgaacag ccactcaccg gcttcggtgg ttagcggcgt
     1801 cagcccggcc gtcacgagag ccagcatgag gaccagccac accaggcggc ggcgcgccgc
     1861 gggccagatg ccgcacagga tttccagcag cgcggttagc ggagccagca ccaccatcgc
     1921 gtgaacgagc aggacgtggg ccggcagccc cgagatcgtg ttcatcagtg ctcctcgcgg
     1981 tcgaataggt gacgacgata gcgtgctcat cctggcacga aactggcagt cgactcgttc
     2041 aggcgcgcgc ggcgttgact aggtcgagcg tcgcctgcgt cccggcagcc agcgcgtcac
     2101 gctcggcgtc ggtgatcgtc gcgcacaccc agtcccagtg cgccgacacc gtgctgccga
     2161 cggccggcgg cggcgccaac gagacaccgt ccttgctcca gcgcaccttc tccggggccg
     2221 gatcgcccag acgcagcgcg tcgttgtcga acatcagcgg ccgggacacc agcacggcat
     2281 actccgcatc aacataatcg acggtgcccc aacggattcg gcagtcctgc atgaccttca
     2341 ccggtgtcgt cgggtcgccc ccgaggaagc ggacccacgg gtacacgacg aacacgtgga
     2401 agctgtggtg cgccgacact ccggcggacg actggagatc gccgagcaag cccgtcactt
     2461 gtcccgcgaa cgcccgacgg agccgggtca gcaggtcgga gggatcgacc cggtccagca
     2521 gcgggccgcc cacccagtag ctgcgcacca cctcgtcgtt aagcggatcg gcgatgccgg
     2581 ccgcgtcagc gattgcctgc aaatagggcc aggcaccgtc gaactcgcgg gcgtgcgcgg
     2641 cgacctcgac gggatcctca ccgcgcagca gcaccgacgc gtcgggtggc ccgcagtacc
     2701 cgagctcgtt cggcgggaac gcgtaacgcg cgaacatcgc gtagcccggg atggactcag
     2761 gcagtgcagt cattcagcag atccgaggca gctgttcacc gatcggcatg tcgacgactc
     2821 gggtgccgcc gagcgccgtg cgggcgacca ccatgccggc atgctccgcg acacacgtgc
     2881 cgacggcggc cgcgcgcaca ccgagcgggt gttcgcgcat cgccgcgagc accttgtcga
     2941 cgtcctcggc gggcacgaac gcgatcagct tgccctcgtt ggcgacgtag agagggtcga
     3001 ggccgagcag cccgcacgcg tcgcgcacct cggccggcac cggcaggctc cgctcgacaa
     3061 gggacacacc gactttggcg gtcttggcga tctcgttgag cgtagcggcc accccgccgc
     3121 gggtgggatc gcgcagcacg tggatgtcgg cgcccgtgtc gatcatcgcg gccaccagtc
     3181 ggtgcagcgg cgcggtgtcg ctagcgaccg tggtgccgaa ctccagcccc tcgcggcagc
     3241 tcatcaccgc gaccccgtgc acgccgatgt cgccgctgac gatcacgacg tcaccgggac
     3301 gcgcgcgttg cggccggatg tcggcgcgct cgtcgaccag gccgacgccg gcggtgttga
     3361 tgaacacccc gtcgccgtgt ccggagtcga cgactttggt atcgccggtg accagcttca
     3421 cgcctgccgc cagcgcggcg gtgccgaccg cctgtgcgac acggccgatt tcgtccagcc
     3481 gtgtgccctc ctcgaggatg aacgcggtcg acatcatcag cggctgcgcg cccgccatcg
     3541 cgagatcgtt gaccgtgccg ttgactgcta ggtcaccgat actgccgccg gggaacacca
     3601 gcggcttgac cacgaacgag tcggtggaga acgcgagccg aactccgccg acgtccatca
     3661 ccgccgagtc gcccatgccg gcgtcggcgg ccgggccgaa cgcgggcagg aacaggtgct
     3721 cgatcaactc cgccgacatc gcgccgccgc cgccgtgccc catcacgatg ttgggcgcgt
     3781 cgcgcagcgg cgccgggcac acccacgagt ccatgtctat ttgtgctcct cgcgtccgcg
     3841 cgtctatttg tgctcctcgc gtccgcgcct caatagtcgc aggtgactgc tgcggtgtct
     3901 caggcatgcg tgacctcgag ccgacggtac aggtagtacg cggcgcacgc gccctcggac
     3961 gaaaccatcg tcgcgccaag gggattgcgt ggcgtgcact gcttgccgaa cgcggcgcac
     4021 tgatgcggct tgatcagccc ttgcagcacc tcgcccgacc ggcacacgga cgactcctcc
     4081 gtgtggatgt cggtgacggc gaagcggtac tcggcgtcga actcgcggta ggcgggggag
     4141 agccgccagc cgctttgcgg gatcatgccg atcccgcgcc aggcgcggtc ggtgacctcg
     4201 aagacatcct gcagcatcgc cttggccggg ctgttgccgg ccgcctggac ggcgcggggg
     4261 taggcgtttt ccagctcatg acgacccgat tcgagctgaa tcacggtgcg gcggatgccc
     4321 tccaggatgt ccagcggctc gaagccggtg accacgatcg ggatcttgta cttctcggtg
     4381 agtggcggat actcgtcggt gcccatcaca cagcacacgt ggcctgccgc caggaacgcc
     4441 tgcacccggc aggtcggcga atccatgatc gctgcgatcg ccggcggcac cagcacgtgc
     4501 gacaccagca gcgaaaagtt ctcgatgccg cggcgtttgg cctcgaacac cgtcatcgcg
     4561 ttggccgggg cggtggtctc gaacccgatg ccgaagaaca ccacctgctt gtcggggttg
     4621 tcgacggcga tggtcaacgc gtccagcggc gagtacacca cccgcacgtc accgccttcg
     4681 ctcttgattc ggaacaggtc cttctcgctg cccggcacgc gcagcatgtc gccgaacgag
     4741 cagaagatga cgtcggggcg cgacgcgatc tcgagggctt tgtcgatgat ctccaggggc
     4801 gtgacgcaca ccgggcagcc tggcccgtgg atcatctcga tctgctcggg cagcagttgg
     4861 tcgatcccgt gtcggatgat cgaatgtgtc tgtccaccac agacttccat gatcgcccac
     4921 cgctgggtgg tggcggcctt gatttgctcg acgaggttgc ccgccagttc aggatcgctg
     4981 aactcgtcca gatacttcat gacttcacct caccgtttcc gttggtgtcc ggctcgggca
     5041 tgccggcctg tcgtgcggcc agctcgaacg cgtcaccgaa ttcttccttc atgacgccga
     5101 ggtgttcgaa ctcggcgagc gtgcgcatgg ctgattcctc gtcgagccgc tggatcgcga
     5161 acccgacgtg gacgatcacg tattcgccga cctgcgcgtc gggaatgtat tgcaggcaca
     5221 cgtctttctt gatgcccccg aagtccacga cggacatcag cgttccgtca cggtcctcca
     5281 agctcagaat cttgcctggt acggctagac acatcggtgt cctcccttgc taattctctt
     5341 cgcgcaagcg ctcatctgtg ccctcacacc gaattcccgg ccagcagttg gcccagcgcg
     5401 ataccgccgt cgttgggcgg aacccgccga tgggtgatga cttcgaagcc gcaccggcgc
     5461 agaccggtca ggctcagctg cagcagcagc gcgttctgga acaccccgcc ggacagcgca
     5521 acggtgttgt gcgacgtcgc ttcccgaacg tcttcggcca ggcgaccgat cagctcggcg
     5581 acggcgcggt ggaaccggac gccgatgacg ccgggctgca cgccggcgcg gtagtcgcgg
     5641 acgattgcag cgagcaccgg agcggggtcg atgatcgccg gctcggcgct gtcgtccagg
     5701 acgaaggcat attctgcggc accgcagaga gtttcgcggg ccagtccctc caactcgatc
     5761 gccgcctccg cctcgtagtc gacggtgtgg cggactccgg ccagcgacga caccgcgtcg
     5821 aacagccggc ccatgctgga ggtcgggaca cagccccagc cggtgtccag ctggtgcgcg
     5881 agcaccttct gctcggcggg cgggcaggcg gccaccggcg cgaggtcgcc gtcccacggg
     5941 atgccggcga cccacagatg agccagtgcc atccggtagg gccgcagtac gctgacgtcg
     6001 ccgccggcca acggcacgta cttcagttga gccagccgtc ggaatccctt gtagttggcg
     6061 agcagcacct ccccacccca caccgcggcg tcggttccgt aacccgttcc gtcgaaggcg
     6121 aatccgatca cctgctcgga cccgtcatac ccgtgctcgg ccatcaccgc ggcgatgtgg
     6181 gcgtgatgat gttgcaccgt gcggaccggg cgtccgtcgg cattgcggtg cgcccaaccg
     6241 gtcgaccggt acagcgggtg agcgtcggcg accagcacgg ccgggtcgac accggtcagc
     6301 gcccgtaggt gcgcctgcgc ggagtcgaac gccgacagcg tggcaacgtc gtccatgtcg
     6361 ccgatgtgct ggctgagcca ggcgtagcgc ccgtcggcga ctgccatcgc gttcttcagg
     6421 tccgcgccga cggcgagcgt cggcggcagc ggcaccggca gcgccaccgg aagcggtgca
     6481 tagccgcgcg agcggcggat cggcaattcg gtgccgtcga ggacccggac caccgagtcg
     6541 tcacacggaa ccaggatcgt ccggtcgtgc atcaaccagc cgtcggccag gtcggccagc
     6601 cgcgccacca cgtcgtcgtc gtagcagatc ggctcgccac caaggtttcc cgacgtcatc
     6661 accagcacgt cgggcccagg ctcgtcgtcc tgcaggccga acagcagcac gtgcagcggc
     6721 gtgtaggtca tcatgatgcc gagatcgggg ttgcgcggtg cgacggaatc ggcgacaccg
     6781 gagtgggcca gccgcggcaa cagcacgatg ggccgttgcg gcccgcatag cactcgttcg
     6841 gcgggctcgt cgacctccgc gatgcgccgg gccgtcgcca agtcgggcac catcaccgca
     6901 aacggcttgt ctccgcggcg cttccgtcgc cgcaactctg cgaccgccgc gtcgttgcgc
     6961 acgtcgcaag cgaggtggta tccaccgatg cccttgacgg cgagtacgcc gccgtcgcgc
     7021 aagagcgtgc gggcggcgcg caaagcgctt tcccggcgga cggtgtcgcg gcccggcgtc
     7081 acgtagctca gggtcgggcc gcacgccggg cagcacaccg gttgggcgtg gaagcggcga
     7141 tcggcggggt cggcgtattc gcgtgcgcag tcggcgcaca tctcgaatgc ggccatcgtc
     7201 gtggtggccc ggtcgtaggg cagcgagccg atgatcgtga accgcgggcc gcagttggtg
     7261 cagttgacga acggatgccg gtagcgccgg ttgcccgggt cgcggagttc cgctgcgcag
     7321 tccgcgcaca tcgcgacgtc gggggaggcc agtgtgcggc cggtaccgga ccgcgacgtg
     7381 tccgcgatcg taaagccggt cccgcccagc accggaatat cttctgtggc aatcgattcg
     7441 atgatcgcca gcggcggcgg ctggtcgcgc agccggtgca ggaactcatc gatggctgcg
     7501 gcgtctcctt cgacctcgat gatcgcgccg gagctgtcgt tgcgcacaca gccggagagg
     7561 ccgagcgcgg ccgaggtggt gtacacgaag ggccggaagc cgactccttg caccactcct
     7621 cggacgcaga cgcgtaaccg ttgccgatcg gtcatggcga cgcctcgggg tcgcggccga
     7681 tcagcttcag acccgccatc gctgcgtctg cgcctgcgcg gtcggtcttt tcgaccacga
     7741 agcccatgtg gatgatcacc cagtcgcccg ggccgaacgt ttcctcgggg agcatgccga
     7801 tgctgacctt gcggtgctcg ccggcgacgt cgacgagcgc gagctgccca ccgtaaccct
     7861 ccagcatgcg gaccacttgt cccgggatgc ccaagcacat gtcagctgcc tcccgtcgcg
     7921 gcgcgcgccg acaacgtcga cagcacctcg gcgacggcgc cgacggcacc gtcgacagcc
     7981 gcgtcgaccg acggcgacag gccgatgccc tcgtcgacgt tctcgacctc acagcccacc
     8041 accacagtgt acggcggggt accccccagc gcggcgaggc tggcgaacac cgcggccgga
     8101 tccatgctgt gggcatccaa aaccggtgtc gtcacaattg attcgtggtc ggcctcgaac
     8161 atgtgcaggg ttcccggcga tccgcggctt ggcaccgcgt cgacgagcac aagagcatcc
     8221 cagtcgtcga ggaggtcgta ggccaggtgc attccgcgga ttccgtagtc ctcgacgcgc
     8281 gcatcagtgc cggcgagccg ttgaccggca cgcttgatca cctcggggcc gaacccgtcg
     8341 tcgccgagga agatgttgcc gatgcccgct accagaatcc gggacgtgat gtcactcctg
     8401 tcatacatct tgcggatctt gtcttacatc ttgcggatct tcatgtagcg ccgcgcatcc
     8461 ggtatggaac cgacgccgac tgcgacgccg aagaggaccg ccgcggcgac cacacccacg
     8521 gcaacccatc ccactacatc cattgcgaac tcctttcggt gagcggttca acttcatcgg
     8581 gagcgaagta caaatagcgg ccgtaccagt cgtgcagatc ggcggccggg tcgtcgtcga
     8641 cgacgacccc gacgtgctgg tttccgtcga catcctcgtg caccgacgtg acgtgcgcgg
     8701 tctttccgct gaagaagatg tcctgcgcgt cggcgcgccg tgacggccgc agccggacgc
     8761 gactgccgcg ggacacacga acgccgttga cgaggacggc atcgatctcg ggctggacgg
     8821 cggtgtcggc cagcgggtcc caccagtcga ccccgtcggg gatctcgggg atgagcgatg
     8881 agccgcttgc gcgaagagca gagggcgacg atgactcttc ccgtgcgtgc gggttcctca
     8941 gcacaccatg cagattgagc atcgcctcgg gtgacatcga gtcacagcgg tcgatgatct
     9001 gagcggccag cggatcggtg gcccgggcct gcgccttctc ctcgtcggtc atcgtcatca
     9061 cgcgcagcgt gagaatctcg tcgatctctg tggagtcgta cagcgcgcct tcgctttgtt
     9121 cggcgatctc cggatggtcg taaaggatga tcggcgacgc gagcatcagg ctgtggtcgc
     9181 cgggcggccc ggccagaacc gggaagcagc ggtgctgacg gcatcgcgcg accgcgtcgc
     9241 cggccagcgg cggcgggtcc agcagcgaga cgaactcacc gtcgatggcc tcggcgatca
     9301 gatgcgtgcc gatgaacgag gcggcgatcg cgttgtcctt gttccccggc ggcgcaccgg
     9361 tattgcgcac ctggatgctg accagcccga gattgccgtc gagttccgcg gccagggtca
     9421 actcaccggt gagctctctg cggctgcgca ccagacggcc accgtcgacg gcttcggtgt
     9481 ccgtaccgcc ggccaccgta agcggaatct tctgtcccgc ttcgagatcc gtgacgggga
     9541 acggtccgaa cgacagttcg cgctccaccg cctcgtccca cgtgagccac gtctggccgt
     9601 cggccgtcaa ctcgtcgacg gtttcgaaaa gcccgtcgcc ggtgtcccgt tccgcggtgc
     9661 gccgctgcaa ctgcaggaac cggaccacca ccgacagcga cgcggtaccc ccgtccagca
     9721 gcacctgagc cgacagcgtg tcgttttcgc cgatgccccg ctcctcggcc cgctgcggac
     9781 ccagcacgcc gaactgccag cgcgactggt tctttcgcga attagcccgg tacgggtaga
     9841 gcagatagcc ctcgtagagc accgcgtcgg cgatggtgcg cacccggtcc cacccgccgc
     9901 tcatggctgc acctcctggg acgatgtctc gagcagtgac gtgaccgcgt cgtcgaaccc
     9961 gagcatgcca cgctcggact tataggccgc cagcgcgtcg agcgtgtcgc ggttcaggcg
    10021 cacccagccg gtgttcgggt agtgctggtc catcaggctc cgccagaccg acacgggcag
    10081 gtcgtagcgg tcctcgcggt cccacggcac ctgctggacg gcaaagccgc gctcaccctg
    10141 cgtgaaaacc gttccgctga acaggaattg cagggggatg gtgccgtcgc gcagcgcatg
    10201 catgtacttg gccgcggtga cctcgaagtc gtaggtgcat tcgagcggca tctctacctg
    10261 ggtggcgccc gagaacccgg gcaccatgac cgcgcagtgc agccacacga acgtccgctg
    10321 ggtggtgctc caacgttcgc gggggccgaa aaggtccagc agcccggccg cttcctcgtc
    10381 ggaatagcgg cgccgcaacg ggtcgatgcg tacctggcag cgcagcgcga tcgcgtgcac
    10441 cgggtcgtcg ccgaccgcag ccacgccgac ctgcgcggcg agcaccggcg tcaccgcata
    10501 cggctgcggg gacacgtcga cgaccgcgta cgtcacctcg gtcatgcgcg atccgccttc
    10561 gcttctcgct tgccggtcac cgcggcacga ccttactgcg ggcggcgacc tgctcgaaga
    10621 actcggagat gaactctcgg acctgctgac cgccgtcgaa cccgtgccac agcagccgca
    10681 gccggccgac gaactcgtag caggcgtcga tcggcacgag gaaagactcg ggagcgccac
    10741 cctcctcgtc gggcactcgg acgatcagcg cctcggtgtc gtcggcgagc atgtccaccc
    10801 gcgggtcgga ttcgcgaatg ttgttccacg ccgccaagtc cagctccgat tcggtcgcac
    10861 cggccggacc cgggtaaaac gcgaccgtgc ggcccagcgc ggagttgcgg aagaagaacg
    10921 ccaggccgac cgggatctgc agcgcttccc attcccgacg gtcgagcgcg aagtccggaa
    10981 aggtcaggca gcggtccggg atcgcgcggt agcgcagctc cgcattcgtg tcggtgaaca
    11041 gcagatagca gcctcggcaa acgcacatca gttgccgggc ttcgacattc acgacgtgct
    11101 ggtgctcgtc ggcgatcggc tcggtgcaca tctcgcagcg ttcgcccgcc ggctgcggcg
    11161 cgttgcggtt ggccctgatg cgggccagga cgtcgtaggc gccggtcatg tggccaccgc
    11221 ccgagccaac gccatcgaca gcacgccgtc gcgctccagc aacgggatcg gctccagatg
    11281 cgcgtcgctg tggccgttcc cgtcgacgca ccgcccggcg tgcacgacgt cgaagtgcgc
    11341 gcgacagcgg ggacatcgca gcaccggtgc gttcgttccc atcatcttgt gcagtgc
//
LOCUS       DASWBF010000286         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1215201,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000286 DASWBF010000000
VERSION     DASWBF010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215201"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF874_10365"
     CDS             <1..204
                     /locus_tag="VF874_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176459.1"
                     /translation="ARFDEIYAQLPNDTARLLVETDIETGLRWGELTELRPADIDTST
                     RVLTVSRVVLARIHRVLIGSGVP"
     gene            284..1612
                     /locus_tag="VF874_10370"
     CDS             284..1612
                     /locus_tag="VF874_10370"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX9176460.1"
                     /translation="MRASHDLSRVSVNFTETNLIPNAGLLPAAMLAQRIDLAGLVDQR
                     LQLARHGAGSGSKALTVIGSVLAGGDSIDDTAVLRAGAAGILFDDTRAPSTIGSWLRA
                     HKWSHVRQHDAICRELLARLWAAGAGPADLTAPLMFDLDSSIVPVFGRGKQGAAFGYT
                     KVRGYHPQFATCAQTGMVLFSRLRGGAAGSARGAKSFLTETVSRLRNAGATGELTVRA
                     DSAFFSKAVLHTAVKLNVRFSVTIRQDKKIRAAIDAIDESAWQPIPYWLSTPETSGAD
                     VAETSYTAFSGKDAMTVRLIVRRVRPTPGSQLALFTTWDYHAFVTNRDGEFLEIEADH
                     RRHAIVEQRIAELKSAGLAHLPSGKFMANAVWLALSVMAHNLGKAVGRLAGPDLDSAT
                     AATLRRKVFTVPGRLVSSARRLALRLPESWPWATAIEQAIEKIQAIPLRC"
BASE COUNT          315 a          633 c          602 g          271 t
ORIGIN      
        1 gcccggttcg acgagatcta cgcgcagctc ccgaacgaca cagcccggct gcttgtggag
       61 accgacatcg aaaccgggct gcgctggggc gaactcaccg aactccgccc cgccgacatc
      121 gacacctcca cccgcgtcct caccgtgtcc cgcgtcgtct tagcccggat ccaccgcgtc
      181 ttgatcggta gcggcgtgcc gtgaacgcgc agatgccctc gtaccaggga cgattggtct
      241 tgcgacggac acaatcggaa ccgcgggaga gggcatctga ctggtgcgag catctcatga
      301 cctgagccgg gtgtcggtga acttcaccga gacgaatctg atcccgaacg ccgggctgct
      361 gccggccgcg atgctggccc agcggatcga cctggctggg ttggtggacc agcggctgca
      421 gctggcccgg cacggcgccg gcagcggcag caaggccctc accgtgatcg ggtcagtcct
      481 ggccggtggc gacagcatcg acgacaccgc ggtcctgcgt gccggtgctg ccgggatcct
      541 gttcgacgac acgagggccc cgtcgacgat cgggtcgtgg ctgcgggcgc acaagtggtc
      601 ccacgtccgg cagcacgacg cgatctgccg cgagctgctg gcccggttgt gggcagccgg
      661 tgccggcccg gccgacctga cggcgccgtt gatgttcgac ctggactcct cgatcgtgcc
      721 ggtgttcggc cgcggcaagc agggcgctgc gttcggctat acgaaggtcc gcggctatca
      781 cccgcagttc gccacgtgcg cgcagaccgg gatggtgctg ttcagccggt tgcgaggtgg
      841 cgctgccggt tcggctcgcg gcgcgaagtc cttcctcacc gagacggtca gccgactgcg
      901 gaacgccggc gcgacggggg agctcaccgt ccgggccgac agcgcgttct tcagcaaggc
      961 cgtgctgcac acggcggtga agctcaacgt ccggttctcc gtcacgatcc ggcaggacaa
     1021 gaagatccga gctgcgatcg acgcgatcga cgagagcgcg tggcagccca tcccgtactg
     1081 gctgagcacc ccggagacct ccggcgcgga cgtcgcggag accagctaca ccgcattcag
     1141 cggcaaggac gcgatgaccg tccggttgat cgtcaggcgg gtccgtccga cgccgggtag
     1201 tcagctcgcg ctgtttacga cctgggacta ccacgcgttc gtgaccaacc gggatggtga
     1261 gttcctcgag atcgaggccg accaccgtcg gcacgcgata gtcgagcagc gcatcgccga
     1321 actcaaaagc gccggcctcg cgcacctgcc gtcaggaaag ttcatggcca acgcggtctg
     1381 gctcgccctg agcgtgatgg cccacaacct cggcaaggcc gtcgggcgcc tcgcaggccc
     1441 cgacctcgac agcgcgaccg cggccacctt acggcgcaag gtgttcaccg tcccgggtcg
     1501 tctggtcagc agcgccagac gcctcgccct gcgcctgccc gagtcctggc catgggccac
     1561 cgccatcgag caggcgatcg agaagatcca ggccatccca ctccgctgct gaacaacacc
     1621 cccatctcaa cgaccaggac ctcggagaag ccggcagacc ggcgactcaa gcacgcccca
     1681 cctcggcatc agctgaaacg accaagatca ggccatcgag atcgacgtca gaaggtcacg
     1741 cggtggatcc gggctaagga tctggctccg gtgggctcaa gaggtacgag gagcccgact
     1801 ctgcaatcat ggggacgcct c
//
LOCUS       DASWBF010000287         7414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_91983, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000287 DASWBF010000000
VERSION     DASWBF010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91983"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1258)
                     /locus_tag="VF874_10375"
                     /pseudo
     CDS             complement(1..1258)
                     /locus_tag="VF874_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(1334..2764)
                     /locus_tag="VF874_10380"
     CDS             complement(1334..2764)
                     /locus_tag="VF874_10380"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX9176461.1"
                     /translation="MRVHACNRRSLLWSPVASVIDTVVNLAKRRGLVYPSGEIYGGTR
                     SAWDYGPLGMELKENIKRQWWQAVVSGRDDVVGIDSSIILPREVWVASGHVEVFHDPL
                     VECLNCHKRHRQDHMQEAYAAKKGLDDPESVPMTEIVCPDCGTKGQWTEPREFNMMLK
                     THLGPIETDEGLHYLRPETAQGIFVNFANVVTTSRKKPPFGIGQIGKSFRNEITPGNF
                     IFRTREFEQMEMEFFVEPATAPEWHQYWIDTRLQWYVDLGIDLENLRLWEHPKDKLSH
                     YSDRTVDIEYKFGFQGNPWGELEGVANRTDFDLKTHSEHSGVDLSFYDQNTETRYIPY
                     VIEPAAGLTRSFMAFLIDAYTEDEAPNAKGGMDKRTVLRLDPRLAPVKAAVLPLSRHA
                     DLSPKARDLAAELRKCWNVEFDDAGAIGRRYRRQDEIGTPYCVTVDFDSLDDHAVTVR
                     ERDKMTQDRVSIDGVSDYLASRLRGC"
     gene            2780..3106
                     /locus_tag="VF874_10385"
     CDS             2780..3106
                     /locus_tag="VF874_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9176462.1"
                     /translation="METISSLVNAPAPSREVLETASDILRALSAPVRIAIVLQLLESQ
                     RCVHELVDALGVPQPLVSQHLRILKAAGVVQGERSGREVLYRLVDQHLAHIVTAAVTH
                     AGEDAT"
     gene            3103..3501
                     /locus_tag="VF874_10390"
     CDS             3103..3501
                     /locus_tag="VF874_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX9176463.1"
                     /translation="MTGAGVRSTRQRAAITALLESLDDFRSAQEIHDELRRRGEGIGL
                     TTVYRTLQSMSAAGAVDTLRTDTGESVYRRCSTHHHHHLVCRNCGFTVEVQGGQVEAW
                     AAEVAAAHGFSEESHTIEIFGTCATCAAKG"
     gene            complement(3498..3881)
                     /locus_tag="VF874_10395"
     CDS             complement(3498..3881)
                     /locus_tag="VF874_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176464.1"
                     /translation="MTLQQRLFDVLDGVVSIRKEDDGALTVQHDGTLASLRVVDIAEG
                     LELISLSQILAWDVPLDRRIRARIAEHSRSTMLGTVTLIERKRIGEVLLRYNFPGSGL
                     TDDALRTLILLVLSTGADVRRALMD"
     gene            complement(3904..4794)
                     /locus_tag="VF874_10400"
     CDS             complement(3904..4794)
                     /locus_tag="VF874_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl diphosphate synthase"
                     /protein_id="HEX9176465.1"
                     /translation="MIRGAERKRQRFPQLDPAPDDYPTFPDKSVWPVPFPDLPPAPGG
                     GPRRPPQHTSKAAAPKIPADQLPHHVAIVMDGNGRWATQRGLHRTDGHKMGEAVLIDV
                     TCGAIEIGIKHLTVYAFSTENWKRSTEEVRFLMGFNREVVRRRRENLNAIGVRMRWVG
                     SRPRMWRSVIKEFEIAEDMTLHNDVITINYCVNYGGRTEIVEATKEIARLAAAGQLNP
                     ERITEATLAKHLHRSDIPDVDLFIRTSGEQRASNFMLWQSAYAEYVFQDKLWPDYDRR
                     DLWAACEEYVNRNRRFGRAE"
     gene            complement(4778..5584)
                     /gene="recO"
                     /locus_tag="VF874_10405"
     CDS             complement(4778..5584)
                     /gene="recO"
                     /locus_tag="VF874_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX9176466.1"
                     /translation="MRLYRDRAVVLRQHKLGEADRIVTLLTREHGLVRAVAKGVRRTR
                     SKFGARLEPFAHIDVQLHPGRNLDIVTQVASIDAFATDIVSDYGRYTCACAVLETAER
                     LAGEERAPVPALHRLTVGALRAVADGARPRELVLDAYLLRAMGIAGWAPALTECARCA
                     APGPHRAFHVAAGGSVCVHCRPAGSSTPPVGVLDLMSALHDGDWDFAQATPQSHRSHA
                     SGLVAAHLQWHLERQLRTLPLVERAVRVDRTVGDHSVALVRQDMARDSGS"
     gene            5647..7113
                     /locus_tag="VF874_10410"
     CDS             5647..7113
                     /locus_tag="VF874_10410"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9176467.1"
                     /translation="MERFPTLAGQLHQLATGAATSDQLVRRSLHAINASQSTLNAFRV
                     VLTKQALTDAADADRRRAAGDRAALLGVPIAVKDDVDVAGVPTAYGTEGHVRPATQDA
                     EVVRRLRAAGAVIVGKTNTCEFGQWPFTSGPGFGHTRNPWSREHTPGGSSGGSAAAVA
                     AGLVPAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFHGIAVYGVLARTVA
                     DAALLLDVASGNAGGDLHKPTPIRLTDHAHAAPGPCRIAISLKFPFTGFRSRLHPEIH
                     AAMDKVADQLEAMGHTIMAGDPSYGLRLGRSFLARSTAGLLDWADRVGDGVTLDPRTV
                     SNLRLGRLLSQRALQRARRDEAIAARRSGRIFNHVDVMLAPTTAQPPPPVGAFDHLGS
                     FRTDRTMITHCPLTWPWNVLGWPSINVPAGFTSDGLPIGVQLMGPANSEPLLVSLAAE
                     LEGVNGWAVKQPDVWWGTPSKNQACQAVSDRAASSWRP"
     gene            complement(7055..>7414)
                     /locus_tag="VF874_10415"
     CDS             complement(7055..>7414)
                     /locus_tag="VF874_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX9176468.1"
                     /translation="PDGELTDEPEEVLMAELIREAALEGVRDELPHSLAVVIDEVTPR
                     PDRDDLVDVHAVLYVERDSQKGIVIGKGGARLREVGTAARTQIEKLLGTKIYLDLRVK
                     VAKNWQRDPKQLGRLGF"
BASE COUNT         1196 a         2495 c         2408 g         1315 t
ORIGIN      
        1 acacgttctc tccgccggag atgatcatgt ctttgagccg atcgacgatg aacacgaagc
       61 cctcgtcgtc catgtaggca gcatcgccgc tgtagtacca accgtctgac agcacggccg
      121 ctgtctcctg tggcttattc cagtagccca gcatgatcat cggccccttg gccgcgactt
      181 cgccgatcgt gccacgcggc acttcctggc ggttctcgtc gacgatcttc acctcggccg
      241 tgtgtgcggc ttggccgcag gacttgaggc ggccggcata cggtccgtcc agtgtcgcgt
      301 agcgcggcgg cagcacggtc aagatcggcg ccgcttcggt catgccgtag ccctggacga
      361 actggctgtt cgggaaaacc tggagtgcct tgcgcagcac gccctcggcc atcggcgagc
      421 cgccgtattg gatgtacttg aggctcgaaa gatcaaagcc gtccaccgtg gggtgattga
      481 tcagcatgtt gatcatggtt ggcacgtata cggcgtgcgt caccttctcg tgctccattg
      541 tctgcaggca gtcggtgacg tcgaagcgcg gcacgaagac atggcaccca ccgcagaccg
      601 tgacgccgaa ggtcgcagag ccgtcggcaa gatggaacat cggggcggag tgcaggaagg
      661 tcatgtcctg gtcgtagtgc acaccggcaa tgatgttcat tgcgttccac acaagattgt
      721 tgtggctgag catgacgccc ttggacttgc ccgtcgtgcc gccggtgtaa aagagccctg
      781 caagcgtgtc gcccccggcg ccgacgtcgg aaaccggggt cgcggccgcg agttcctcgt
      841 agggccgcat gcccgcgggc ggcgcgtcgt cgtcgagata gaacagcgcc tcgacagtcg
      901 gcatctgtcc gcccaacgca gcggcatgcg cctgcatcgc gccgtcgacg aacagcacgc
      961 gcgcctagga atcgtcaagc acgtactgga tctccggcgt tgcgagccgc gtgttgaccg
     1021 gcaccatgac tccagcgacc cacgggatcg cgtacatcaa ttccaagtag cggtcgctgt
     1081 tgagcgcgag gatcgccacc tgatcgccgg gtcgcacgcc gacgtgttcg agtccgccgg
     1141 cgatccgcgc gacacggtca gccgtctccg accacgtgcg tcgccgatcc ctgaacaccg
     1201 tggattgtgc tcgcggccaa atctgcgctg cgcgccgcaa cccctgcgtc agatacatga
     1261 cgaccaccac ctatgttggg cgtcatcata agtgcgtgca gacggcgttt gacagctttc
     1321 gccgaggaag tggtcagcaa cctcgcagcc gggatgcgag gtaatcagaa accccgtcga
     1381 tggatacccg atcctgcgtc atcttgtcgc gttcccgcac ggtcacggcg tgatcgtcga
     1441 gcgagtcgaa atcgactgtc acgcaataag gtgtgccgat ctcgtcctgg cggcggtacc
     1501 gtcggccgat cgcgccggcg tcgtcgaact cgacgttcca gcatttacgt agctcggcgg
     1561 ccagatcgcg cgccttcggg gacagatcgg cgtgccggga cagcggcagc accgcagcct
     1621 tcaccggcgc cagccgaggg tcgagtcgca gcaccgtgcg tttgtccatg ccgcccttgg
     1681 catttggcgc ctcgtcctcg gtgtaggcgt cgatcagaaa cgccatgaac gaccgtgtca
     1741 gacccgctgc cggctcgatc acgtacggga tgtagcgggt ttcggtgttc tggtcgtaga
     1801 acgacaggtc cacaccggag tgctcggagt gcgttttcaa atcgaaatcg gtgcggttgg
     1861 cgacgccctc cagctcaccc cacggattgc cttggaagcc gaacttgtac tcgatgtcga
     1921 ccgtgcggtc cgaatagtgc gagagcttgt ccttggggtg ttcccacagc cgcagattct
     1981 ccagatcgat gcccaggtcg acgtaccact gcaaccgggt gtcgatccag tactggtgcc
     2041 attccggcgc ggtcgccggc tcgacgaaaa attccatctc catctgctcg aattcgcggg
     2101 tgcgaaagat gaaattccct ggagtgatct cgttgcggaa gctcttgccg atctggccga
     2161 ttccgaacgg cggcttcttg cgtgacgtcg tcaccacgtt ggcgaagttg acgaagatgc
     2221 cctgcgcggt ttcgggcctc aggtagtgca ggccctcgtc ggtttcgatc gggccgagat
     2281 gggtcttgag catcatgttg aactcgcgcg gctcggtcca ctggcctttg gtgccgcaat
     2341 ccgggcacac gatctcggtc atcggcaccg actcggggtc gtcgagaccc tttttcgcgg
     2401 cgtatgcctc ctgcatgtgg tcctgacgat gccgcttgtg gcaattcagg cactccacca
     2461 gcgggtcgtg gaacacctcg acgtggcctg aggccaccca cacctcacgc ggcaggatga
     2521 tcgagctgtc gatgccgacg acgtcgtcgc ggccggagac caccgcttgc caccactggc
     2581 gcttgatgtt ctctttgagc tccataccca acggcccgta gtcccaggct gaccttgtgc
     2641 cgccgtagat ttcaccggac gggtacacga gcccgcgtcg cttggccagg ttgacgacgg
     2701 tatcgatgac ggatgccacg ggtgaccaga gtaacgaccg cctgttgcat gcgtgaacgc
     2761 gcatgtaatc tggcttctaa tggaaaccat ttccagtttg gtaaacgccc cagcgccgtc
     2821 gcgagaagtg ctcgagaccg ccagcgacat cttgcgcgcg ctgtccgcgc cggtgcggat
     2881 cgcgatcgtg ttgcagcttc tcgagtcgca gcgctgcgtg catgagctgg tcgacgctct
     2941 gggggtgccg cagccgctgg tcagtcagca cctccggatc ctgaaggccg ccggcgtggt
     3001 gcaaggcgag cggtccggcc gtgaggtgct ctaccggctg gtggaccagc acctggcgca
     3061 catcgtgacg gccgcggtga cccacgccgg cgaggacgcc acgtgaccgg cgcgggagtc
     3121 cgcagcactc gtcagcgcgc ggcgatcacc gctctgctgg agtccctcga cgatttccgc
     3181 tccgcccagg aaatccacga cgaactccgc cgccgtggtg agggcatcgg gttgaccacc
     3241 gtgtaccgca cgttgcagtc gatgtcggcg gccggtgccg tcgacacgct gcgcaccgat
     3301 accggcgagt ccgtgtaccg ccgctgttcg acgcaccacc atcatcatct ggtgtgccgc
     3361 aactgcggtt tcacggtcga ggtgcaaggc ggccaggtgg aggcgtgggc cgctgaagtt
     3421 gctgccgccc atggcttttc ggaggaaagc cacaccatcg agatcttcgg gacgtgcgcc
     3481 acctgcgcgg ccaaaggcta gtccatcagc gcgcggcgca cgtcggcacc cgtggacagc
     3541 accagcagga tcaacgtacg cagcgcgtcg tcggtgagcc cgctgcccgg aaagttgtag
     3601 cgcagcagca cttcgccgat ccgcttcctt tcgatcaacg tcaccgtgcc cagcatggtg
     3661 ctgcgggagt gttcggcgat cctggctcga atccgccggt ccagcggcac atcccaggca
     3721 aggatctgcg acaaggagat caactccaga ccctcggcga tgtcgaccac ccgcagcgac
     3781 gccaatgtgc cgtcatgctg caccgtcaac gcgccgtcgt cttccttccg aatcgacacc
     3841 acgccgtcga gcacgtcgaa aagccgctgc tgcagcgtca tcacgctctg ccgctcaggt
     3901 acatcattcg gctctgccga agcggcggtt gcggttgacg tattcctcgc aggccgccca
     3961 caagtcccgg cggtcatagt cgggccacag tttgtcctgg aacacgtact cggcgtacgc
     4021 cgactgccag agcatgaagt tgctggcccg ctgctccccc gacgtccgga tgaacaggtc
     4081 gacgtccgga atgtcggacc gatgcaggtg tttggccagc gttgcctcgg tgatccgctc
     4141 gggattcagc tgcccggccg cggccagccg cgcgatttcc tttgtggcct cgacgatttc
     4201 ggtgcggccg ccgtagttca cgcagtagtt gatggtgatg acgtcgttgt ggagagtcat
     4261 gtcctcggcg atctcgaatt ccttgatcac gctgcgccac atccgtggcc gcgagccgac
     4321 ccatcgcatc cggacgccta tcgcgttgag gttctcacgt cggcgccgca cgacttcacg
     4381 gttgaacccc atcaggaagc ggacctcttc ggtgctgcgc ttccagttct cggtggaaaa
     4441 tgcgtacacg gtcaggtgtt tgatgccgat ctcgatggcg ccgcaggtga cgtcgatgag
     4501 cacggcctca cccatcttgt ggccatcggt gcggtgcagc ccgcgctggg tggcccaacg
     4561 accgttgccg tccatcacga tcgcgacgtg atggggcagt tggtcggcgg ggattttcgg
     4621 agccgcggcc ttcgacgtgt gctgcggcgg tcgtctcggg ccgcccccgg gcgccggcgg
     4681 cagatccggg aacgggacgg gccagaccga tttgtcaggg aaggtcgggt agtcgtcggg
     4741 cgccgggtcc agttgcggga accgctgtcg cttgcgctca gctccccgaa tcacgcgcca
     4801 tatcctgcct gaccagcgcc acgctgtggt cgccgactgt gcgatcaacc cgtacggcgc
     4861 gctccaccag cggcaatgtc cgcagctgcc gttcgagatg ccactgcagg tgcgcggcca
     4921 ccagcccgct ggcatggctg cgatgcgact gcggcgtggc ctgggcaaag tcccagtcac
     4981 cgtcgtgcaa agcggacatc agatccagca cccctaccgg cggcgtggac gatcccgccg
     5041 gtcggcagtg cacacacacg ctcccacctg ccgcgacgtg aaacgcccga tgcggcccag
     5101 gcgccgcgca gcgggcgcac tcggtgagcg ccggcgccca cccggcgatt cccatggcgc
     5161 gcagcagata tgcatccagc accagctcgc gcggacgcgc tccgtcggcc actgcgcgca
     5221 acgcgccgac ggtgagccgg tgcagcgccg gaacgggagc ccgctcctcg ccggccagcc
     5281 gctcagcggt ttccagcacc gcgcacgcac aggtgtagcg gccgtagtcg ctgacgatgt
     5341 cggtggcgaa cgcgtcgatg gaggccacct gcgtgacgat gtcgaggttg cggccggggt
     5401 gcagctggac gtcgatgtgg gcgaacggct ccagccgcgc gccgaacttg ctgcgcgtgc
     5461 gccgcacgcc cttggcgacc gcgcgcacca gcccgtgctc acgcgtgagc agggtgacga
     5521 tccggtcagc ctcgccgagc ttgtgctggc gcagcacgac tgcccggtcc cggtacaacc
     5581 gcattggttc agtttcgcac cgcaaacaga cataactggt acgcagcgcc ggttaatgtc
     5641 tggcttgtgg agcggttccc gaccctcgct ggtcaactgc accagctggc caccggcgcc
     5701 gcgacctccg accagctggt gcgccgcagc ctgcacgcca tcaacgccag ccagtcgacg
     5761 ctcaacgcgt tccgggtggt actcaccaag caggcgctca ccgacgccgc cgatgccgat
     5821 cgccgccgcg ccgccggcga ccgtgccgcg ctgttgggcg taccgattgc cgtaaaggac
     5881 gacgtcgacg tcgccggtgt gcccacggcg tacggcaccg agggccacgt ccgcccagcg
     5941 acccaggacg ccgaggtggt gcgtcgactt cgcgcggccg gggcggtgat cgtcggcaag
     6001 accaacacct gcgagttcgg gcagtggccg ttcaccagcg ggccgggctt cgggcacacc
     6061 cgcaacccgt ggtcgcggga gcacacgccc ggcggttcgt caggcggaag cgctgcggcc
     6121 gtggctgccg gcctggtgcc ggcggcgatc ggctccgacg gcgcgggcag catccgcatc
     6181 ccggcggcct ggacacacct ggtcgggatc aagccgcagc gtggccgcat ctccacgtgg
     6241 ccgctgccgg aggccttcca cggcatcgcg gtctacggcg tgctggcccg caccgtcgcc
     6301 gatgcggcgc tgctgctcga cgtggcgtcg ggcaacgcgg gcggcgacct gcacaagccg
     6361 acgccgatcc ggctgaccga ccatgcgcat gccgcgccgg gaccgtgccg gatcgcgata
     6421 tcgctgaagt ttccgttcac ggggttccgc tcccggctgc accccgagat tcacgctgct
     6481 atggacaagg tcgccgacca gctggaggcg atggggcaca cgatcatggc cggcgacccg
     6541 tcatacgggc tgcggctggg gcgcagcttt ctggcccgct cgacggccgg gctactggac
     6601 tgggccgacc gcgtcggtga cggcgtcacg ctcgatccgc gcaccgtctc caacttgcgg
     6661 cttggtcggc tgttgtcgca acgggcgctg cagcgggcgc gacgcgacga ggccatcgcg
     6721 gcgcgccgct ccggacggat cttcaaccac gtggacgtga tgctcgctcc gaccaccgca
     6781 caaccgcccc cgcccgtggg cgcgttcgac cacctcggtt cgttccgcac ggatcgcacc
     6841 atgatcacgc actgcccgtt gacgtggccg tggaatgtgc tcggctggcc gtcgatcaac
     6901 gtgccggccg ggttcaccag cgacggacta ccgatcggtg tgcagctcat gggcccggcg
     6961 aacagcgagc cgctgctggt atcgctggcc gccgagttgg agggcgtcaa cggctgggcg
     7021 gtgaagcagc cggacgtgtg gtggggaacg ccctctaaaa accaagcctg ccaagctgtt
     7081 tcggatcgcg ctgccagttc ttggcgacct tgacgcgcag atcgagatag atcttggtac
     7141 ccagcagctt ctcgatctgg gtgcgcgcgg cggtgcccac ttcacgcagc ctggctccgc
     7201 ccttgccgat gacgatgccc ttctgactgt cgcgctcgac gtagaggacc gcgtgcacgt
     7261 cgaccagatc gtcgcggtcc ggccgcggag tcacctcgtc gatcaccacg gccaacgaat
     7321 gcggcagctc gtcgcgaact ccttccagcg cggcctcgcg aatcagctcg gccatcagca
     7381 cctcttcggg ttcgtcggtg agctcgccgt ccgg
//
LOCUS       DASWBF010000288         6964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_495491,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000288 DASWBF010000000
VERSION     DASWBF010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495491"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VF874_10420"
     CDS             <1..1357
                     /locus_tag="VF874_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX9176469.1"
                     /translation="WKRSPGGLVTALEPLLRRRRGAWVGWPGVVDDAVDMADEPIALD
                     ELQLHPVRLSADDIAQYYEGFSNATLWPLYHDVIVKPTYHREWWDRYVDVNRRFAEAA
                     SRAAAKNATVWVQDYQLQLVPKMLRELRPDLTIGFFLHIPFPPVELFMQLPWRTAIVE
                     GLLGADLVGFQLSGGAQNFLFLARRLVGANTSRGSIGVRSRFGEVEYGSRVVRVGAFP
                     ISIDSAELDHKGRERSIRHRAREIRAELGNPRKVLLGVDRLDYTKGIDVRLRAFSELL
                     AEGRAKRDDTVLVQLATPSRERVESYKALREDIERQVGHINGEYGEVGHPVVHYLHRP
                     VPRDELIAFFVASDVMLVTPLRDGMNLVAKEYVACRSDLGGALVLSEFTGAAAELSQA
                     YLVNPHDPDSVKDAIEAAINQSLEDGRRRMRALRRQVLAHDVDLWARSFLDALAEKPE
                     S"
     gene            complement(1354..1836)
                     /locus_tag="VF874_10425"
     CDS             complement(1354..1836)
                     /locus_tag="VF874_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HEX9176470.1"
                     /translation="MRWLITGVAGLLAAAFVALAGVGGWLYWDRVEMRGEQATRAELA
                     PLAKDQVPKILGYDYQTVETSLTAAYPMMTPDFRRQYEDRTTKEIIPQAREQHLVSQV
                     NVVGVGVMDAHRNSGSVMVYINQTYLAKDREPLYSGSRVRVNYERIDGQWLISDIKPI
                     "
     gene            complement(1836..2525)
                     /locus_tag="VF874_10430"
     CDS             complement(1836..2525)
                     /locus_tag="VF874_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HEX9176471.1"
                     /translation="MTTTESGPQQAARRAVRAAGPANGGTAETTTVRLEKSPEEERQG
                     RKPIRKAGPPPRRLAHLKLVATVALAAGVLAVAGLVTAVTLMFFENRDAQARQEQDQR
                     FVDTASQLAVNMYSFNQNTVEDSVKRVVNSTSGPLHDMLARDNHTEVLEQFFRSMGGS
                     SEAVVTGAALESKDADVGTATVLVSVRVTTTDANGNNQPSTPSRMRITVRQEDDGGMT
                     GYDLKWPDGGN"
     gene            complement(2525..4273)
                     /locus_tag="VF874_10435"
     CDS             complement(2525..4273)
                     /locus_tag="VF874_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9176472.1"
                     /translation="MLDRLARIQLTIFAIVTVLCVGAIAVFYLHVPARLGIGAYDITA
                     DFASGGGLYKNANVTYRGVTVGRVEAVTLTNDSVEAHMRLNSQFKIPENVTATVKSVS
                     AVGEQYVDLVPPQNPSNAALHDGSRIDQDRTAIGQDIEGLLQQADALVSSISNTRLQD
                     LLRETFKAFNGSGPELARLIQSARLLVDEANANWTETSQLIDQVGPFLQAQIRSGDDI
                     RSLSDGLARFTTELRQADPQLRALLVTAPGAADEASNTFAGIRPSFPVLAASLANLGR
                     VGVIYNKSIEQALVTFPALMSALITLAGGIPYDEGGKLDFKVDLGDPPPCLTGFLPPQ
                     FIRPPADQTVRDLPKDMYCKVPQNDPSAVRGARNYPCQEFPGKRAPTVQLCRDPRGYV
                     PLGNNPWRGPPVPTGTPVEDPRNTLPPNKYPYIPPEADPDPGYPVAPGAVPPGVQPGP
                     GPAPHQPWPVENPPVDGAPPPPLTAWVPPAPYPDAWPAPANPQPPPLSPGPYPPVVPP
                     PGPAPAPAPPPDPGALPPAAVTPPGVSGTSYSSYDQRSGTFIDANGNAAVFGAGAQNL
                     KPQENWYDLMKDPRQT"
     gene            complement(4273..5481)
                     /locus_tag="VF874_10440"
     CDS             complement(4273..5481)
                     /locus_tag="VF874_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9176473.1"
                     /translation="MTPNSFQQALCRKLRRGGIRTVALGSGMAVLAGCQFGGLNSLNM
                     PGTAGHGPGSYSITVEVPDVSTMPQNSPVMVDDVTVGSVSGVEAVQRSDGTFFAAVKL
                     SLDENVKLPANSVARVAQTSLLGSQHIELAPPLGQQRVGELRDGSTISLANGSRYPTT
                     EEVLSALGVVVNKGNLGALGDITDEAYAAVAGRQGQFVDLIPRLAEFTAALNAQTNDI
                     ISTAEGLNRVAGTLAAHKDSLSRALDTLPGALRVLNDNRKNIVDTFAALQSFATVASH
                     VLSQTKDDIAADLKDLYAVIKPINDNRADFISDLDLLPTFPFTTRYLRRAIRGDYLNV
                     FVTFDLTIRRLGETIFTTSYFDPNMPHLNDVVNPPDWLIGSFANLNGQAADPFKIPPG
                     TASGQEVPPS"
     gene            complement(5478..6905)
                     /locus_tag="VF874_10445"
     CDS             complement(5478..6905)
                     /locus_tag="VF874_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9176474.1"
                     /translation="MRWVRYGVIAALAVVLVAGIYLVWPGRAGHKVIGYFTSAVGLYP
                     GDGVRIVGVPVGSIESIEPQPQAVKVTISVKDAVKVPADAHAVIMAPNIVSARFIQLA
                     PAYSSGPVMADGATIGLDRTAAPVEWDEVKSELTKLSSSLGPQAGSMQGPLSEFINQA
                     ADTFDGNGESFRQALRELSQTAGRLGDSRTDLFGTVKNLHILIDALSKSNEQIVQFSG
                     HLASVSQVLADSSTNLDNTLGTLNQALADVRGFLQDNNSALISQIDKLSQLTKLFSDQ
                     SDDVEQILHVTPNGLANFYNIYDPAQGAASGLLSLPNFANPVQFICGGVFEGSLFVDN
                     IKRTEICRERMAPVLSRLAMNYPPILFHPINTITAYKGQIIYDTPATEAKTQTYLPYL
                     QWVPSEGRNPPREGATGVDALMLPPNVAPGPPPPAQPQFLQPAGPAPGPAPAAPVAPD
                     TDGPFPAEGVPPMQPVAPQQPGAGG"
BASE COUNT         1133 a         2222 c         2339 g         1270 t
ORIGIN      
        1 gtggaaacgc agtcccggcg gattggttac ggcattagag ccgctgctgc gtcgccggcg
       61 cggggcctgg gtcggctggc cgggtgttgt cgacgatgcc gtggacatgg ccgacgagcc
      121 catcgccctg gacgaactgc aacttcatcc ggtacggctc agcgctgacg acatcgccca
      181 gtactacgag ggcttttcca atgccacgct gtggccgctg taccacgacg tcatcgtcaa
      241 accgacctac caccgcgagt ggtgggaccg ctacgtcgac gtgaaccgcc gcttcgccga
      301 agccgcgtca cgcgctgcgg cgaaaaacgc caccgtgtgg gtacaggact accagctgca
      361 gctggtgccg aagatgctgc gggaactgcg accggacctg accatcggct tctttctgca
      421 catcccgttt cctcccgtcg agctgttcat gcaattgcct tggcgcacag cgattgtcga
      481 gggactgctg ggcgccgatc tggtcggctt ccagctttcc ggcggcgcgc agaacttcct
      541 cttcctggca agacggctcg tcggtgccaa cacatcgcgc ggatcgatcg gtgtccgttc
      601 acgtttcggc gaggtggagt acgggtcgcg ggtggtccgg gtgggcgctt ttccgatctc
      661 gatcgactct gctgaactcg accacaaagg cagagagcgg agtatccgcc accgcgcgcg
      721 agagatccgc gccgaactcg gcaacccgcg caaggtactg ctcggtgtcg atcgcctgga
      781 ctatacgaag ggcatcgacg ttcggctgag agcattttcc gaactcctgg ccgaggggcg
      841 cgccaaacgg gacgacaccg tgctcgtgca gctggcaacg cccagccgcg aacgcgtcga
      901 aagctacaag gcgcttcgcg aggacatcga gcggcaggtc gggcacatca acggcgagta
      961 cggcgaagtc ggccatccgg ttgtgcacta cctgcaccga cccgttccgc gcgacgagtt
     1021 gatcgcgttc ttcgtcgcca gcgacgtcat gctggtcacg ccgctgcgag acggcatgaa
     1081 tctcgtggcc aaggagtacg tggcgtgtcg cagcgacttg ggcggcgccc tcgtactcag
     1141 tgaattcacc ggcgcggcag ccgaactcag tcaggcctac ctagtcaacc cgcacgatcc
     1201 cgacagcgtc aaggacgcca tcgaagcagc cataaaccag tccctcgagg atggccggcg
     1261 acggatgcgg gcgcttcgcc gtcaggtgct ggcccatgac gtggacctct gggcgcggtc
     1321 atttctcgac gcgctggcgg agaagccgga gtcttagatc ggtttgatgt cgctgatcag
     1381 ccactgacca tcgatgcgct catagttcac gcggacccgg ctccctgaat acagcggctc
     1441 tcggtccttc gccaggtagg tctggttgat atagaccatc accgagcccg agttgcggtg
     1501 ggcgtccatc acgccgaccc cgaccacatt cacctgactg acgaggtgtt gctcgcgcgc
     1561 ctgcggaatg atttccttcg tcgttcgatc ctcatactgg cggcggaagt ccggcgtcat
     1621 catcggatac gccgcggtga ggcttgtctc gaccgtctgg tagtcatagc caaggatttt
     1681 cgggacttga tctttggcga gcggggccaa ctccgcccgg gtcgcctgct cgccgcgcat
     1741 ttcgacgcgg tcccaataca gccaaccgcc cactccggcc agcgcgacaa acgcagcggc
     1801 gagtaatccg gcgacgccgg tgatgagcca gcgcatcaat tacccccgtc gggccacttc
     1861 aggtcgtagc cggtcatccc gccatcgtct tcctgccgca ccgtgatgcg catcctggat
     1921 ggtgtcgacg gctggttgtt gccgttagcg tcggtcgttg tcacgcgcac ggacaccaat
     1981 accgtagcgg tgccgacgtc ggcatctttg ctttccagcg cggcgccggt cactaccgct
     2041 tcggagctac cgcccatcga gcggaagaac tgctcgagga cctccgtgtg gttgtcgcgg
     2101 gcgagcatgt cgtgcagcgg gccactcgtg ctgttcacga ctctcttgac gctgtcctcg
     2161 accgtgttct ggttgaagct gtacatgttc accgcgagtt gtgacgcggt gtcgacgaac
     2221 cgttggtcct gctcttgacg tgcctgcgca tcacgattct cgaagaacat caacgtcaca
     2281 gcggtcacga gaccggcgac cgccaggacg ccagcggcca acgccacggt cgcgaccagt
     2341 ttgaggtgcg cgagccgacg aggcgggggt ccggctttgc ggatcggctt acgaccctga
     2401 cgttcttcct cgggcgactt ctcgagccgg accgtggtcg tttcggccgt tccgccgttg
     2461 gccggaccgg ccgcacggac cgcacggcga gccgcctgct ggggcccgga ttcggttgtg
     2521 gtcatcaggt ttgcctcgga tccttcatga ggtcgtacca attctcttgt ggcttcaggt
     2581 tctgcgcacc ggcgccgaag accgcggcat tgccgttcgc atcgatgaag gtgccgctcc
     2641 gctggtcata gctgctgtat gaggtcccgc tcacccctgg cggtgtcaca gcggccggcg
     2701 gaagcgcccc cgggtcgggc ggcggcgcag gcgccggcgc aggcccgggc ggtggcacca
     2761 cgggcgggta gggccccggt gacagcggcg gcggctgcgg gttcgccggt gccggccatg
     2821 catccgggta cggtgccggg ggcacccacg ccgtcagcgg cggtggcggt gcgccgtcga
     2881 caggcggatt ctcgaccggc cacggctggt gcggggccgg gcccggcccg ggctgcacgc
     2941 ccggcggcac tgcaccgggc gcaaccgggt agcccggatc cggatccgcc tcgggcggga
     3001 tgtacggata cttgttgggc ggcagggtgt tccgaggatc ctcgaccggt gtaccggtcg
     3061 gaaccggcgg cccgcgccac gggttgttgc cgagcggaac atagccgcgc ggatcgcggc
     3121 agagctggac cgttggcgca cgcttacccg ggaattcttg gcatgggtag ttcctggccc
     3181 cacgcaccgc actcggatcg ttttgcggca ctttgcagta catgtccttc ggcaggtcgc
     3241 ggacggtctg gtcggcgggt ggccggatga actggggtgg caagaacccg gtcaagcagg
     3301 gcggcgggtc gccgagatcc accttgaagt ccagcttgcc gccctcgtcg tacgggatcc
     3361 caccggccag ggtgatcagc gccgacatca gcgccgggaa ggtgaccagc gcttgttcga
     3421 tcgacttgtt gtagatgaca ccgacgcggc cgaggttggc caagctggcg gccagcaccg
     3481 ggaacgacgg ccggatgccc gcgaacgtgt tgctggcttc gtccgcggcc ccgggcgcgg
     3541 tcactagtag cgcgcgcagc tgcggatccg cctggcgcag ttcggtggtg aaccgtgcca
     3601 gcccgtcgga cagggacctg atgtcgtcac cactgcggat ctgggcttgc aggaacgggc
     3661 ccacctgatc gatcagctga gacgtttcgg tccagttggc gttggcctcg tcgacgagca
     3721 gtcgcgccga ctggatcagc ctcgccagct ccggcccgga accgttgaac gccttgaatg
     3781 tttcccggag gagatcctgc aaacgggtgt tgctgatgct gctgaccaac gcgtcggcct
     3841 gttgcagcag cccttcgatg tcctggccga tggcggtccg gtcttggtcg atgcgtgagc
     3901 cgtcgtgcag cgcggcgttc gacgggttct ggggcggtac cagatccaca tactgctcgc
     3961 ccaccgcgga gacgctcttg accgtggccg tgacgttttc gggaatcttg aactgactgt
     4021 tgagccgcat gtgcgcttcg acgctgtcat tggtcagcgt cacggcttcc acccgcccga
     4081 ccgtcacgcc gcggtacgtg acgttggcgt tcttgtacag cccgccaccg gatgcgaagt
     4141 cggcggtgat gtcgtaggcg ccgattccca ggcgggcagg cacgtgaagg tagaacacgg
     4201 cgatcgcgcc gacgcagagg acggtgacga tcgcgaagat cgtcagttgg atgcgcgcca
     4261 atcgatccag catcagctcg gcggcacctc ctggcccgac gcggtgccgg gcggaatctt
     4321 gaacgggtcg gctgcctggc cattcaggtt cgcgaacgag ccgatcaacc agtccggcgg
     4381 attgacgacg tcgttgaggt gcggcatgtt cgggtcgaag tacgacgtgg tgaagatggt
     4441 ctcgccgagg cggcggattg tcaggtcgaa tgtgacgaac acattgaggt aatcgccccg
     4501 gatggctcgc cgcagatacc ttgtcgtgaa cgggaaggtg ggcagcaggt cgaggtcgga
     4561 gatgaaatcg gcccggttgt cgttgatcgg cttgatcacc gcgtagagat ccttgaggtc
     4621 ggccgcgatg tcgtccttgg tctgcgacag aacgtgcgac gccacggtcg cgaagctctg
     4681 cagcgccgcg aacgtgtcga cgatgttctt gcggttgtcg ttgagaaccc gcaacgcacc
     4741 cggcagcgtg tccagagcgc ggctcagact gtccttgtgc gcggccagag ttcccgccac
     4801 ccggttcagt ccttccgcgg tcgagatgat gtcgttggtc tgcgcgttca gcgccgcagt
     4861 gaactcggca agtctgggaa tcagatcgac gaactgtcct tgccgaccgg cgaccgcggc
     4921 gtaggcctca tctgtgatgt cacccaacgc accgaggttg cccttgttca ccacgacgcc
     4981 gagggctgac agaacttctt ctgtggtggg gtaccggctc ccgttggcca gcgagatcgt
     5041 ggagccgtcg cgaagctcac ccacccgctg ctgaccgagc ggcggggcca actcgatgtg
     5101 ctgggacccc agcagcgacg tctgcgccac ccgcgcaacc gaattggcgg gcagcttgac
     5161 attctcgtcg agcgaaagct tcacggccgc aaagaacgtg ccgtcggacc gctgcaccgc
     5221 ctcgacaccg gagacgctgc cgaccgtcac gtcgtcgacc atgaccggcg agttctgggg
     5281 catcgtcgac acatcgggca cttcgacggt gatgctgtag gagcccggcc catgacccgc
     5341 cgtgcccggc atgttcaacg agttcaggcc gccgaactga catccggcca gcacggccat
     5401 gccggagccg agcgccaccg tgcggatccc accacggcgt agcttgcgac acagggcttg
     5461 ttggaaggag tttggagtca tccgcccgcc cccggctgtt gcggtgcgac gggctgcatc
     5521 ggcgggacac cctcggccgg gaacggcccg tcggtgtccg gcgcgacagg cgccgcgggc
     5581 gccggcccgg gcgcgggtcc ggctggctgg aggaactgcg gctgcgccgg tggcggcggc
     5641 ccgggcgcga cgttcggcgg caacatcagc gcgtcgacgc cggtcgcgcc ttcacggggc
     5701 gggttgcggc cctccgacgg aacccactgc aggtaaggca gatacgtctg cgtcttagct
     5761 tcagtggccg gagtgtcgta gatgatctgc cccttgtacg cggtgatggt gttgatcggg
     5821 tggaacaaga tgggcgggta gttcatcgcc agccggctca gcacgggggc catccgctcc
     5881 cggcagatct cggtccgttt gatgttgtcg acgaacaggc tgccctcgaa cacgccgcca
     5941 cagatgaact gcaccgggtt ggcgaagttc ggcagcgaca gcagtcccga cgccgccccc
     6001 tgcgcggggt cgtagatgtt gtagaagttc gccagcccgt tcggggtgac gtgcagaatc
     6061 tgttcgacgt cgtcggactg gtcggagaaa agcttcgtca actgcgacag cttgtcgatc
     6121 tgactgatca gcgcagagtt gttgtcctgc aggaatccgc gaacgtcagc caaagcctgg
     6181 ttaagcgtgc cgagcgtgtt gtcgaggttc gtagaactgt cggcgagcac ctgcgacacc
     6241 gacgccaggt ggccgctgaa ctgcacgatc tgctcgttgc tcttcgacag cgcgtcgatc
     6301 aggatgtgca ggttcttgac ggtgccgaac aggtcggtgc gggaatcgcc cagccgcccg
     6361 gccgtctggg acagctctcg tagtgcttgg cggaacgatt cgccgttgcc gtcgaaggta
     6421 tcggcggcct ggttgatgaa ctcactgagc ggcccctgca tcgaaccggc ctgcggccca
     6481 agcgatgaac tgagcttcgt cagctccgac ttcacttcgt cccattccac cggcgccgcc
     6541 gtgcgatcca gtccgatggt cgcgccgtct gccatgacgg gaccgcttga gtaggcgggg
     6601 gcgagctgaa tgaagcgcgc ggacacgatg ttcggcgcca tgatgaccgc atgtgcgtcg
     6661 gccgggacct tgaccgcatc tttgaccgat atcgtcacct tgaccgcctg cggctgcggc
     6721 tcaatggatt cgatgctgcc gaccgggacg ccgacgatgc ggacgccgtc acccgggtac
     6781 aacccgaccg ccgacgtgaa gtacccgatc accttgtgac cggcacggcc cggccaaacc
     6841 aggtagatgc cggcgacgag caccaccgcg agggcggcga tgacgccgta gcgcacccac
     6901 ctcattccgg gagatgccgg ctgcgcgttc atggtgactt cggcctgatg atcaagcgct
     6961 cgga
//
LOCUS       DASWBF010000289        11793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_495714,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000289 DASWBF010000000
VERSION     DASWBF010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495714"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF874_10450"
     CDS             <1..337
                     /locus_tag="VF874_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-hexose 4-ketoreductase"
                     /protein_id="HEX9176475.1"
                     /translation="KQLKSKDMAMQLTDKAKALLAKRGFDPVLGARPLRRTIQREIED
                     ALSEKILFEEVGPGQLVTVDVDNWDGEGAGEDAAFTFTGTRKPAKAADEPDLAQAGAA
                     GSHSATATE"
     gene            670..894
                     /locus_tag="VF874_10455"
     CDS             670..894
                     /locus_tag="VF874_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HEX9176476.1"
                     /translation="MTSSDAPRARTAKVRAVDLSATSAALWLGGTVFLALLVVYFVGF
                     DQGAVSVFGSDSHVHEFIHDARHLLGFPCH"
     gene            898..1638
                     /locus_tag="VF874_10460"
     CDS             898..1638
                     /locus_tag="VF874_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HEX9176477.1"
                     /translation="MEKRVIWRGLLAGAIGGVVAYLFARAFVEPAIGRAIDFEAAQSA
                     PGHEEGAELFTRAVQANVGMGFGVVAFGMAMGALFAVAFVVAWSRLGDIGPRGLSILL
                     AAGAFGAIVFVPWLKYPPNPPAVGHEETLGDRTGLYLLMVLVSLLAAVGAVWLGRRVV
                     SQFGGWSATLIGVGAYVVAIAVVMLVLPPVAEVPDDFPATALYDFRLYSMGTQFVMWA
                     TIGVVFASLTARLLDEHSTTGRAPSLIP"
     gene            1635..2186
                     /locus_tag="VF874_10465"
     CDS             1635..2186
                     /locus_tag="VF874_10465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9176478.1"
                     /translation="MTEVVRLTLVSHAMTDAMSAARFPVDEPLNAVGRRQLDIGINFG
                     ATEFAAVSGPEQRCRQTAELLGIRPTVEPRLSDLNCGRWRGEVLSGVRPADLTDWLTD
                     PTSAPHGGESLVDLMHRVAGWLESLTASPSRAVAVTHPGVIRAAIVVALDAPAKSFWR
                     IDVAPGSRTEMHFRGQAWTLRSL"
     gene            complement(2212..3591)
                     /locus_tag="VF874_10470"
     CDS             complement(2212..3591)
                     /locus_tag="VF874_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9176479.1"
                     /translation="MRGPAPRRSAVRARLRAAAALAAVAVATAPVCSCSTPSASSVSA
                     DAGVTISMNTPPGFRAKQTVDMLNSDWPIGPVGVRTLAAPDKVDTVRDTMENLWWERP
                     FTLSGVDYYAGLVTLHLVNSFGAGQDIEIHTDENGMVDRFDAELQQPKINSWVDVDTE
                     LAKSGARYSYQVAKVDDGRCDRVAGTNTGQSLPLASIFKLYVLLAVADAVKAGKLSWD
                     DQLTITLQAKAVGSSGLEKLPPGTQVSVRTAAEKMIANSDNMATDLLIEKVGTAAVER
                     ALVEAGHHDPESMTPFPTMHELFSVGWGEPDLREQWRNAVATGLPQARAQLLAQTNSR
                     PYEPDPTRTHTPASTYGAEWYGSAEDICRVHAALQSRAVGVTAPVRDIMSAIPGIDLD
                     PQQWPYIGAKAGNLPGDLTFSWYAVDSGGQAWVVNFQLNWPRFHGPSAGAWLISIAKQ
                     VFAILPRDG"
     gene            complement(3566..3883)
                     /gene="mhuD"
                     /locus_tag="VF874_10475"
     CDS             complement(3566..3883)
                     /gene="mhuD"
                     /locus_tag="VF874_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycobilin-forming heme oxygenase MhuD"
                     /protein_id="HEX9176480.1"
                     /translation="MAVVKINAIEVPPDAGPELEKRFAHRAHAVDSQPGFLGFQLLRP
                     VKGEDRYFVVTQWESEEAFQAWATGPAIEAHAGQRANPVATGASLLEFEVVLDVAGTG
                     AEA"
     gene            3882..4688
                     /locus_tag="VF874_10480"
     CDS             3882..4688
                     /locus_tag="VF874_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9176481.1"
                     /translation="MGFRLPCGHHERVSADLLTYCGGHGQPLVLVHGLMGRGRTWRRQ
                     LPWLTALGRVYTYDAPWHRGRDVAHPHPIRTERFVGDLRDAVASLGRPVILIGHSMGG
                     LHAWCLAADRPDLVRALVVEDMAPDFRGRTTGPWEPWLHALPVEFPSAQQVFDEFGSV
                     AGQYFLDAFDRGATGWRLHGHVERWIEIAAEWGMRDYWESWRAVRVPTLLIEAGNSVT
                     PPGQMQQMHESGWATDYLRVPEAGHLVHDEAADVYRDVVTAFLASLAERA"
     gene            complement(4662..4916)
                     /locus_tag="VF874_10485"
     CDS             complement(4662..4916)
                     /locus_tag="VF874_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176482.1"
                     /translation="MTVLITLSILMAPFAVAVALGWAAHRSTGVRINLDQFRVSAPMR
                     GRLSGGSPDAPRMQHELDAIRTRFEQQPAWPASGALGERR"
     gene            4988..5977
                     /locus_tag="VF874_10490"
     CDS             4988..5977
                     /locus_tag="VF874_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9176483.1"
                     /translation="MLRSVSVLVLERLAIFEFGVVCEVFGIDRQSDGVPNFDFRVCGP
                     QAGRPLQTSVGAMVIPEHGLDGLAGADLVAVPAVHPARGPHPYPPEALAALRAAAQAG
                     ATILTVCSGAFVAGAAGLLDGRRCTTHWMHAEQLARTYPTAQVDRNVLFVDDGNLITS
                     AGTAAGIDACLHLVRRELGSRITNIIARRMVVPPQRDGGQRQFIDQPVPTHCSPGFAP
                     QLDWILANLDKAHSVATMARHANMSERTFARKFVEEAGTTPMQWVTDQRVLFARRLLE
                     ETDLDVDRIAERCGFGTATLLRHHFRRLVGLTPSDYRRRFACVDAACEQEQSA"
     gene            complement(6003..6911)
                     /locus_tag="VF874_10495"
     CDS             complement(6003..6911)
                     /locus_tag="VF874_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX9176484.1"
                     /translation="MGDIVPPDRPGRQVSIDAAELIGWYDAAQRDLPWRRPGVSPWQV
                     LVSEFMLQQTPVSRVQPIWEEWVDRWPTPSATAAASAADVLRAWGKLGYPRRAKRLHE
                     CATVIARDHADVVPDDVETLLGLPGVGGYTARAIACFAYGRRVPVVDTNVRRVVARAV
                     RGIADAPASARDLVDVAALLPEDRSAPWFSAALMELGATVCTARSPRCGVCPLSTCAW
                     RAAGHPPAEAPARRPQTYAGTDRQVRGRLLDVLRANDFPVQRRQLDMAWLTDTDQRER
                     ALASLLADGLVEQTPDGRYALAGEAR"
     gene            6910..7542
                     /locus_tag="VF874_10500"
     CDS             6910..7542
                     /locus_tag="VF874_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX9176485.1"
                     /translation="MPNTNPSSAWKALKEGNERFVAGQPAHPSQSVDHRATLAAGQRP
                     TAVVFGCADSRVAAEIIFDQGLGDMFVVRTAGHVIDSAVMGSIEYAVAVLDVPLIVVL
                     GHDSCGAIESTLDALDRGVIPRGYVRDLVERVTPSVLTGRREGLTQVDEFEAHHVRET
                     AAQLLSRSTTILERIPTGELAIVGLTYHLADGRVALRASVGDIGEYPACS"
     gene            7603..8373
                     /locus_tag="VF874_10505"
     CDS             7603..8373
                     /locus_tag="VF874_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176486.1"
                     /translation="MLDLEPHGPLPSQIYWRRRALAGGIAVVVIGIVVAVVITVLTGN
                     TGADNKNSAQPADVPASQVPNPQPEVKSPITPLPPAPIQTPTPTAAVMPPPVLKDGDD
                     CPDSTLAVKGIASQPQYVVGEQPKFTMLVTNIGLVACQRDVGAAVLAAYVFSLDNQRL
                     WSNLDCAPSTETLVKKFSPGEQVTTEVTWTGMGSAPQCPLPRQPIGPGTYNLVVQLGN
                     LRSAPVPFIIAQPSAQSETPAPAQPIPGQDPAQVNPVG"
     gene            complement(8380..9492)
                     /gene="disA"
                     /locus_tag="VF874_10510"
     CDS             complement(8380..9492)
                     /gene="disA"
                     /locus_tag="VF874_10510"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEX9176487.1"
                     /translation="MTSTERSQNTKAPLVHPALRETLRRLAPGTPLRDGLERILRGRT
                     GALIVLGYDERVESICDGGFSLDVRFAPTRLRELSKMDGAQVLSTDGSRIVRANVQLV
                     PDPSIPTDESGTRHRSAERTAIQTGYPVVSVSHSMSIVTVYVAGERHVMADSATILSR
                     ANQAVATLERYKSRLDEVSRQLSTAEIEDFVTLRDVMTVVQRLEMVRRISLEIDEHVV
                     ELGTDGRQLKLQLEELVGGNDLARELIVRDYHANPEPPTGEQVASTLDELDQLSDTEL
                     LDLTALSKVFGYPSTAEAQDSAMSSRGYRAMAGIPRLQFAHIDLLVRAFGSLQGLLAA
                     SSSDLQKVEGIGAMWARHIREGLSQLAESTIADRFV"
     gene            complement(9489..10916)
                     /gene="radA"
                     /locus_tag="VF874_10515"
     CDS             complement(9489..10916)
                     /gene="radA"
                     /locus_tag="VF874_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX9176488.1"
                     /translation="MAKARTQHRCSECRHVMAKWVGRCPECGTWGTVDEVPTIKVVGG
                     RAQRSAAPNSAAVPISSIPPGTTRHDPTGVDELDRVLGGGVVPGSVTLLAGEPGVGKS
                     TLLLKVVHQWARAGRRALYVSGEESAGQVGMRAERTGCSHDEVYLAADSDLDNVLAHI
                     DAVRPTLVVVDSVQTMSTTDADGVTGGVTQVRAVTTALMAAAKRSGVAIILVGHVTKD
                     GAIAGPRTLEHLVDVVLHFEGDRQSMLRMVRGVKNRFGAADEVGCFQLLEYGIVGMPD
                     PSGLFLDQRPEPVSGTAVTVTLDGKRPLIGEVQALLIKPQHAIPRRAVSGIDNARASL
                     IAAVLTKRCGAPVNSHDIYLSTVGGMRLTDPSSDLAVAVAMASAWAGLPLPTTTVVIG
                     ELGLAGDLRQVTDMERRLAEAARLGFTTALVPYGVYTVPKGIYAMQARTITEALDLLR
                     DIAVKPKRATTLRPLELAGEPAGES"
     gene            complement(10950..11528)
                     /locus_tag="VF874_10520"
     CDS             complement(10950..11528)
                     /locus_tag="VF874_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176489.1"
                     /translation="MNLFPTRGSALGGGLVAAVLIAATLTGCGSGQQSQTATQEPAVN
                     GTSVGVGNIALRDVRIRAEITSAALQPGESVELLFVASNQSADGNDRLLGITSDIGSV
                     ALKPSNPQIPATGSLIVGKPEGAEAEALQALSDAEKANATVKLSKPISNALTYEFVFK
                     FERAGEAKVAVPVTAGENAPRQQGAAPPGEGH"
BASE COUNT         1865 a         4016 c         3926 g         1986 t
ORIGIN      
        1 gaagcagctg aagagcaagg acatggcgat gcagctgacc gacaaggcca aggcgctgtt
       61 ggccaagcgc ggattcgacc cggtgctggg tgcgcggccg ctgcgccgga cgatccagcg
      121 cgagatcgag gacgcgctct ccgagaagat cctgttcgag gaggtcgggc cgggccagct
      181 ggtcaccgtc gacgtcgaca actgggacgg cgaaggcgcc ggcgaggacg cggcgttcac
      241 gttcaccggc acccgcaagc cggccaaggc ggccgacgag ccggatctgg cccaggccgg
      301 cgccgccggt tcgcacagcg cgaccgcaac cgagtagcgg cgagccacgc caaagcctcc
      361 gaaatggcga cgtttcgggg gctttcgcgt ctttctgatc tgccgtcgcc ctcgagtgtg
      421 cgttttcgta cacttccccc gcgtgtcccg tacgaaatcg cagtgtcgcc gcgaccctcg
      481 ccgttgttca cgcgactacg gtggtgcgtc gttgacctgc tcagcggctg gccctaagct
      541 cctctactat tcgatgggcc gaggaatctg gtgagattcc agaacggtcg cgccactgtg
      601 cacagtcaga cccgacgcct gtcactgccc tgctagcggg acgcgcaatc ccgaaaggag
      661 tacgaccaca tgacatcatc cgacgcgcct cgggcgcgga ccgcgaaggt tcgcgctgtc
      721 gacctgtccg cgacgagtgc agcgttgtgg ctgggcggta ccgtctttct tgcgcttctg
      781 gtggtttact tcgtcggctt cgatcagggt gcggtttcgg ttttcggtag cgacagccac
      841 gtccacgagt tcatccacga tgcccggcac ctgctgggct tcccctgcca ctagaccatg
      901 gaaaagcggg tcatctggcg cggcctgctg gccggcgcca tcggtggggt agtggcttac
      961 ctgttcgcgc gggccttcgt cgaaccggcg atcgggcggg ccatcgactt cgaagcggcg
     1021 cagagtgcgc ccggtcacga agaaggtgcc gaactcttca ctcgcgcggt gcaggccaac
     1081 gtcgggatgg gctttggggt cgtcgcattc gggatggcga tgggagcatt gttcgcggtc
     1141 gcgttcgtgg tggcgtggtc ccgactcggg gatatcggtc cgcggggctt gtcgattctg
     1201 ttggcggccg gagcatttgg cgcgatcgtc ttcgtaccgt ggctgaagta tccaccgaac
     1261 ccgccggcgg tcgggcatga agagacactc ggtgaccgca cggggctcta cctgctgatg
     1321 gtgctggtgt cgttgctggc ggcggttggt gcggtgtggc tgggccggcg ggtcgtgtcg
     1381 cagttcggcg ggtggagcgc gacgctgatc ggcgttggcg cgtatgtcgt cgcgatcgcg
     1441 gttgtgatgc tggtgctgcc gccggtcgct gaagtgcctg acgactttcc tgccacagcg
     1501 ctctacgact tccggttgta ctcgatgggc actcagttcg tgatgtgggc gacgatcgga
     1561 gtggtcttcg cgtcgttgac cgcccggctg ctcgatgagc actcgacgac gggacgggcg
     1621 cccagcctca tcccgtgacc gaggtcgtcc ggctgacgct ggtgtcgcat gcgatgaccg
     1681 acgcgatgtc agctgcgcgg tttcccgtcg acgagcctct caacgccgtt ggccgacggc
     1741 agttggatat cggaatcaac ttcggcgcaa cggaattcgc cgcggtatcc ggaccggaac
     1801 aacggtgtcg gcagaccgca gagctactcg gcattcgccc aacggtcgag ccgcgtctga
     1861 gcgacttgaa ttgcgggcgg tggcgcggcg aagtgctcag cggtgtgcgc cctgcggacc
     1921 tcaccgattg gctcaccgac cccaccagcg caccgcacgg cggtgagtcc ttggtcgacc
     1981 tgatgcaccg ggttgcgggt tggctcgaat cgttgaccgc cagcccgtct cgggccgtcg
     2041 cggtgaccca tcccggtgtg atccgagccg cgatcgtggt ggctcttgac gcaccggcaa
     2101 agtcgttctg gcgcatcgac gttgcgccgg gaagtcgcac cgagatgcac ttccgcggcc
     2161 aggcctggac gctgcgatcg ctctagctcg gcgcgggtta tcacccgcga ctcagccgtc
     2221 ccgcggcaga atcgcgaaca cctgcttggc gatcgatatc agccatgcgc cagcgctggg
     2281 tccgtgaaac cgcggccagt tcaactggaa gttgaccacc catgcctggc cgccgctgtc
     2341 caccgcgtac cagctgaacg tcaagtcgcc gggcaggttt ccggctttgg cgccgatgta
     2401 gggccactgc tgcgggtcga ggtcgatgcc gggaatcgcc gacatgatgt cgcgcaccgg
     2461 tgcagtcacc ccgacagcgc gcgactgcag cgcagcgtgc acccggcaaa tgtcttcggc
     2521 gctgccgtac cattccgcgc cgtacgtcga tgccggagtg tgggtgcgtg tcggatctgg
     2581 ctcgtaggga cgggaattcg tctgcgccag cagctgcgcg cgggcctgcg gtagcccggt
     2641 cgcaaccgcg ttgcgccact gctcccgcag atccggctca ccccagccga ccgaaaacaa
     2701 ctcgtgcata gtcgggaatg gggtcatgct ttccggatcg tgatggccgg cctcgaccag
     2761 cgctcgttcg accgctgccg ttccgacctt ctcgatgagc agatcggtcg ccatgttgtc
     2821 gctgttggcg atcatcttct cggcagcggt gcgcaccgac acctgggtgc cgggaggcaa
     2881 cttctccaaa cccgacgaac cgacggcttt ggcctgcaac gtgatagtga gctggtcatc
     2941 ccacgacagc ttgcccgcct tcactgcgtc ggcaacggca agcagcacgt aaagcttgaa
     3001 aatcgatgcg agcggcaagg attggcccgt gttggtgcct gccacccggt cgcagcggcc
     3061 gtcgtcaacc ttggcgactt gataggagta tcgcgcgccg gacttcgcca gctccgtgtc
     3121 gacgtccacc cacgagttga tcttcggctg ctgtagctcc gcgtcgaacc ggtcgaccat
     3181 tccgttctcg tcggtgtgga tttcgatgtc ctggccagcg ccgaacgagt tgaccagatg
     3241 aagtgtgacg agaccggcgt aatagtcgac gcccgaaagc gtgaacggtc tttcccacca
     3301 aaggttttcc atcgtgtcgc gcaccgtgtc caccttgtcg ggcgcagcga gcgtccgtac
     3361 cccgaccggg ccgatcggcc agtcggaatt cagcatgtcc acggtctgct ttgcgcggaa
     3421 ccccggcggc gtgttcatgc tgatcgtcac acccgcatcg gcgctcaccg acgacgcgga
     3481 cggcgtcgag caactgcata ccggggcagt ggcgacggcg acagcggcca gcgcggccgc
     3541 cgcccgcaac cgcgctcgga ccgcgctacg cctcggcgcc ggtccccgca acgtccaaca
     3601 caacctcgaa ctccagcaac gacgccccag tcgccacagg attggcccgc tgtccggcgt
     3661 gcgcctcgat cgccgggccg gtggcccatg cctgaaacgc ctcttcggac tcccactgcg
     3721 tcacgacgaa gtaccggtcc tcacctttga cgggccgtag cagctggaag ccgaggaacc
     3781 ccggctggct gtcgacggcg tgtgcgcggt gggcgaatcg tttctccagt tcgggccctg
     3841 cgtccggcgg cacttcgatt gcgttgattt tgaccacggc catggggttc aggctaccgt
     3901 gcggccatca tgagagagtg agcgccgatc tgctgacgta ctgcggcggc cacggccagc
     3961 cgctggtgtt ggtgcacggg ctgatgggtc gcggccgcac ctggcgccga cagctgccct
     4021 ggctcaccgc actcgggcgg gtctacacct atgacgcgcc atggcaccgt ggccgcgacg
     4081 tcgcgcatcc gcacccgatc aggaccgaac gtttcgtcgg cgacctccgc gacgctgtgg
     4141 catcgctcgg ccggccggtc atcctgatcg gacactcgat gggtgggctg catgcgtggt
     4201 gcttggccgc cgacaggcct gatctggtca gagcgctcgt cgtcgaggac atggcaccgg
     4261 acttccgggg ccgcaccacc ggtccgtggg agccctggct gcatgcgctg cccgtcgaat
     4321 tcccgagcgc gcagcaggtc ttcgacgagt tcgggagcgt tgcgggccag tacttcctcg
     4381 acgcgttcga ccgcggcgcg accgggtggc gcctgcacgg ccacgtcgag cgctggatcg
     4441 agatcgccgc cgagtgggga atgcgtgact actgggagtc gtggcgggcg gttcgggtgc
     4501 cgacgctgct gatcgaggcc ggtaactcgg tgaccccacc cggccaaatg cagcagatgc
     4561 acgaatccgg ttgggccaca gactacttgc gagtgccaga ggccggtcac ctggtgcacg
     4621 acgaagcggc cgatgtgtac cgcgacgtgg tcaccgcgtt cctagcgtcg ctcgccgagc
     4681 gcgcctgagg ccggccacgc cggctgttgc tcgaagcggg tgcggatcgc atcgagctcg
     4741 tgctgcatcc gcggggcgtc ggggctgccg ccggacaacc ggccgcgcat cggtgccgaa
     4801 acccggaact ggtccaaatt gatgcgcacc ccggtcgagc ggtgcgccgc ccaacccagc
     4861 gccaccgcca ccgcgaaggg agccatcagg atcgaaagcg tgatcaaaac ggtcatggca
     4921 gtaagtcttc gccagtgaac attccgccaa cagtggcagg actgacgtat tgcgataaga
     4981 tactgccatg ctcagatctg tgtccgtgtt ggtgttggag aggctcgcga tcttcgagtt
     5041 cggcgtcgtc tgcgaggtat tcggcatcga ccgccaatcc gacggcgtcc cgaacttcga
     5101 cttccgtgtc tgcgggccgc aagccggccg tccactgcaa acgtcggtcg gcgccatggt
     5161 gattcccgag cacgggctgg acgggctcgc cggcgcggac ctggtcgcgg tcccggcggt
     5221 acacccagcc cgggggccgc acccgtatcc gccagaggcg ctggcggcgc ttcgagcggc
     5281 tgcgcaggcc ggagccacga tcttgaccgt ctgctccggt gcgttcgtcg ccggtgcggc
     5341 cggcctgctg gacgggcgcc ggtgcaccac tcactggatg cacgccgaac agctggctcg
     5401 gacatatccg accgcgcagg tcgaccgcaa cgtgctgttc gtcgacgacg gcaacctgat
     5461 caccagcgcc ggcacggcgg ccggaatcga cgcgtgcctg catctcgtgc gtcgcgagtt
     5521 gggcagcagg atcacgaaca tcatcgcccg ccggatggtg gttccaccac agcgcgacgg
     5581 cggccagcgg cagttcatcg atcagccggt gccgacgcac tgctcaccgg gattcgcccc
     5641 gcaactcgac tggatcctgg cgaatctgga taaggcgcac tcggtggcga cgatggcacg
     5701 ccacgcgaac atgtcggagc gcacgttcgc gcgaaagttc gtggaagagg ccggcacaac
     5761 gccgatgcag tgggtaaccg accagcgggt gctgttcgcc aggcggctgc tcgaggagac
     5821 cgatctggac gtcgaccgca tcgccgagcg gtgcggcttc ggcaccgcga cgctgctccg
     5881 ccaccacttc cgtcgtctcg ttggcctcac gccgtctgat taccgtcgcc ggttcgcgtg
     5941 cgtggacgca gcctgtgagc aagaacagtc agcctgacgc acgcaccgaa ataagatgag
     6001 cctcaacggg cttcgccggc caaggcgtaa cggccgtcgg gggtctgctc cacgaggccg
     6061 tcggccagca gcgaagccaa cgcgcgctca cgctggtcgg tgtccgtcag ccaggccatg
     6121 tcgagctgtc gccgctgtac gggaaagtcg ttggcgcgca acacatccag aagtctgccc
     6181 cgcacctgtc ggtcggtgcc cgcgtacgtc tgcgggcgac gggccggcgc ctcggccggc
     6241 gggtggcctg cggctcgcca cgcgcacgtg ctgagtgggc acaccccgca ccgaggcgac
     6301 cgcgcagtgc acaccgtcgc gcccaactcc atcaacgccg ccgaaaacca tggcgctgaa
     6361 cggtcttcgg gaagcagcgc ggcgacgtcg acgagatcgc gtgcgctcgc cggagcgtcg
     6421 gcgattccgc gcaccgcccg cgcgaccacc cgtcgtacgt tcgtatcgac caccggcacc
     6481 cggcgcccgt aggcgaaaca cgcaatcgca cgtgcggtgt acccgccgac gcccggaagg
     6541 ccgagcagcg tctcgacgtc gtcgggcacc acgtcggcgt gatcgcgcgc gatcaccgtc
     6601 gcgcactcgt gtaaccgctt ggcgcggcgc gggtaaccca gcttccccca tgcccgcagc
     6661 acgtcggcgg cgctggccgc cgcagtggcc gacggcgtcg gccagcggtc aacccactcc
     6721 tcccagatcg gctgaacccg cgacaccggg gtctgctgca gcatgaactc gctgaccagg
     6781 acctgccacg ggctgacccc gggccgtcgc cacggcaagt cgcgctgagc cgcgtcgtac
     6841 cagccgatca gctcagcggc atcgatgctc acctgccgcc ccggtcggtc tgggggcaca
     6901 atgtcaccca tgcccaacac gaacccctcg tccgcttgga aggcactcaa ggagggtaac
     6961 gagcggttcg tcgccggcca gcccgcccac cccagccaaa gtgtcgacca tcgcgccacg
     7021 ctggctgccg gtcagcgtcc gactgcggtg gtgttcggct gcgccgacag ccgggtggcc
     7081 gccgagatca tcttcgacca aggactcggc gacatgttcg tggtccgcac cgccggtcac
     7141 gtcatcgact cggccgtgat gggctcgatc gaatacgcgg tggcggtgct cgacgtgccg
     7201 ctgatcgtcg tgctcggcca cgacagctgc ggtgcgatcg agtccacgtt ggacgccctc
     7261 gacaggggag tgatcccgcg cggatacgtt cgcgacctgg tcgaacgcgt cacgccctcg
     7321 gtgctcaccg gccgccgcga aggattgacc caggtcgacg agttcgaggc gcaccacgtc
     7381 cgggagaccg ctgcgcaact gctgtcgcgg tcgacgacga tcctggagcg gatcccgacg
     7441 ggcgagctgg cgatcgtcgg cctgacgtat cacttggccg acgggcgggt ggcgctgcgc
     7501 gccagtgtgg gcgacatcgg cgagtatccc gcgtgtagtt agcccggcga cacgccgagc
     7561 aacgtcggac ggctcgcggt gacctgggct taccgtgaga acgtgctgga tcttgaaccg
     7621 catggcccac tgccgtccca gatttactgg cgacgccgcg ctctcgccgg gggcatcgcg
     7681 gtcgtcgtga tcggcatcgt cgtcgcggtc gtcatcacgg tgctgaccgg gaacacaggc
     7741 gccgacaaca agaactccgc ccagcccgcc gacgtgccgg cctcccaggt gccgaacccg
     7801 cagccggagg tgaagtcccc gatcacgccg ctgccgccgg cgccgatcca gacgccaacc
     7861 ccgaccgcgg ccgtcatgcc tccgccggtg ctgaaagacg gtgacgactg cccggattcc
     7921 acgctggccg tcaagggcat cgccagccag ccacaatacg tcgtcggcga gcagccgaag
     7981 ttcacgatgt tggtgaccaa tatcgggttg gtggcctgtc agcgtgacgt cggcgccgcc
     8041 gtgctggccg cgtacgtgtt ctcgctcgac aaccagcggt tgtggtccaa cctcgactgc
     8101 gcgccgtcga ccgagacgct ggtcaagaag ttcagcccag gcgagcaggt gaccaccgaa
     8161 gtcacctgga ctggaatggg ttccgcgccg cagtgcccgc tgccgcgcca gccgatcgga
     8221 cccggcacgt acaacctcgt cgtgcaattg ggcaacctgc gatccgcccc ggtgccgttc
     8281 atcatcgccc agccgtcggc gcaaagcgag accccggcgc ctgcccagcc gatcccaggg
     8341 caggatcccg cacaggtgaa tccggtcggc taagccgcat tacacgaacc tgtcggcgat
     8401 cgtcgactcg gccagctgcg acaagccttc gcggatgtgc cgcgcccaca tcgcgccgat
     8461 gccttccacc ttctgaaggt cgctcgaact ggccgccagc agaccttgca gcgacccgaa
     8521 ggcgcgcacc agtaggtcga tgtgggcgaa ctgcagccgc ggtatgcccg ccatcgcccg
     8581 gtagccacgt gagctcatcg ccgagtcctg cgcttcggcg gtggacggat acccgaaaac
     8641 ctttgacagc gcggtcaagt cgagaagctc ggtgtcggac agctggtcga gttcatcgag
     8701 cgttgacgcc acctgctctc cggtgggcgg ctcggggttg gcgtggtagt cacggacgat
     8761 cagctcacgc gcgaggtcgt ttccgccgac cagctcctcg agctgcagct tgagctggcg
     8821 accgtcggtg ccgagttcca ccacgtgctc gtcgatttcg aggctgatgc gccgcaccat
     8881 ctccaggcgt tgcacaacag tcatcacgtc gcgcagcgtt acgaagtctt cgatctcggc
     8941 ggtcgacagc tggcgactga cctcgtcgag gcgagacttg tagcgctcca gggtcgcaac
     9001 ggcctgattt gcccgcgaca gaattgttgc cgagtcggcc ataacgtgcc gctcgccggc
     9061 gacgtacacc gtcacgatgc tcatcgaatg gctaacggac acgacgggat aacccgtctg
     9121 gatcgcggtg cgctcggccg accggtggcg ggtgccggac tcgtcggtgg ggatggacgg
     9181 gtcaggcacc agttgcacgt tggcccgcac gatgcggctg ccgtcggtgg acagcacctg
     9241 tgcgccgtcc atcttcgaca gctcccgaag cctcgtcggg gcgaaccgga cgtcgagcga
     9301 gaagccgccg tcgcagatcg actcgacgcg ctcgtcgtaa cccaacacga tcagcgcacc
     9361 cgtgcggccg cgcaggattc gctccagccc gtcgcgcagt ggcgtgccgg gcgccaggcg
     9421 ccgcagcgtc tctcgcagcg ccggatgcac caacggcgcc ttggtgttct gactgcgctc
     9481 agtcgacgtc atgactcgcc tgctggctcc cccgcaagtt ctagcggacg cagcgtggtc
     9541 gcacgcttcg gcttgaccgc gatatctcgg agtaagtcga gcgcctcggt gatggttcgt
     9601 gcctgcatcg cgtagatccc cttcggcacg gtatataccc cataggggac cagcgccgtg
     9661 gtgaatccga gccgagcggc ctcagcgagc ctgcgttcca tgtcggtcac ctgccgcaga
     9721 tcgccggcca gcccgagctc gccgatcacg acggtggtgg tgggcaacgg caggcccgcc
     9781 caggccgacg ccatcgccac cgccaccgcc aagtccgagg acggatcggt caaccgcatg
     9841 ccaccgaccg tggacaggta gatgtcgtgg ctgttcacgg gcgcgccgca ccgcttggtg
     9901 agcaccgcgg cgatcagcga ggctctggcg ttgtcgatgc cgctgaccgc ccgccgcggg
     9961 atcgcgtgct gcggtttgat cagcagcgcc tgcacctcgc cgatcagcgg gcgcttgccg
    10021 tcaagtgtga cggtcacggc ggtgccggag accggttcgg ggcgctgatc gagaaacaac
    10081 cccgacgggt ccggcatgcc gacgattccg tactcgagca gctggaagca gcccacttcg
    10141 tcggccgcgc cgaagcggtt cttcaccccg cgaaccatcc gcagcatcga ctgccggtcg
    10201 ccttcgaagt gcagcaccac gtccaccagg tgctccagcg tccgaggccc cgcgatggcg
    10261 ccgtccttgg tgacgtggcc gacgaggatg atcgcgactc cgctgcgctt tgccgccgcc
    10321 atcagagcgg tggtcacggc ccggacttgc gtgacacctc cggtcacgcc gtcggcgtcc
    10381 gtggtcgaca tggtctgcac cgagtccacc accaccaagg ttggccgcac cgcgtcgatg
    10441 tgcgccaaca cgttgtcgag gtccgagtcg gcggccagat acacctcgtc gtggctgcag
    10501 ccggtgcgct cggcgcgcat cccgacctgc cccgccgatt cctcgccgga cacgtacagg
    10561 gctctgcggc cggcgcgcgc ccattgatgc acgaccttca gcaaaagggt ggactttccg
    10621 acgcccggct caccggcgag cagcgtcacg gatccgggta ccacgccgcc acccagcacc
    10681 cggtcgagct cgtcgactcc cgtcgggtcg tggcgcgtgg tgcccggcgg gatcgaactg
    10741 atcggcacgg cagccgaatt gggcgccgct gaccgctgcg cgcggcctcc gacgaccttg
    10801 atcgtcggca cctcgtcgac ggtgccccag gtgccgcact cggggcagcg gcccacccac
    10861 ttggccatca cgtgccgaca ttccgagcag cggtgctggg tgcgtgcctt ggccacgccg
    10921 agacggtatc gggtgggtcc gacagatcct cagtgacctt cgccgggcgg cgccgcccct
    10981 tgttgccgcg gggcgttctc accggcggtg acgggcaccg cgaccttggc ctcaccggcg
    11041 cgctcaaact tgaacacgaa ctcatacgtc aacgcgttgc tgatcggctt ggacagcttc
    11101 acggtggcat tcgccttctc ggcgtcggac aacgcctgca gcgcttcagc ttcggcgccc
    11161 tccggcttgc ccacgatcag cgacccggtc gcgggtatct gcgggttgga cggcttgagg
    11221 gcgacggacc cgatgtcgga agtaatgcca agcagccggt cgtttccgtc ggccgactgg
    11281 ttgctggcga cgaacagcag ctcgacgctc tcgcccggct gcagggcggc agaggtgatt
    11341 tccgcgcgga tacgcacatc acggagcgcg atgttgccga cgccgacgct ggtgccgttg
    11401 acggcaggct cctgggtggc ggtctgggac tgctgtcccg atccgcagcc ggtcaacgtc
    11461 gcggcgatca gaaccgcggc gacgagcccg ccgccgagag ccgatccgcg ggtgggaaag
    11521 aggttcactg aggtctcctg ctcggcttga actggcatgg catcggccga ctcacggccg
    11581 ccagcgttcg actatgcaca gtagtaggtc ggcggccggc agcgaagcac gcggcgatta
    11641 ttcgacggtg acaggtcgtc ggcaacaccg cgaccagcta cctgcggcgc tgtcgatgaa
    11701 accgctggca tgcatgtatt cgccgctgtg tcaaccccca atgttcaccc actgtgcccc
    11761 tgacctgcac cgttgttaaa cgcccgtcag agg
//
LOCUS       DASWBF010000290         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1276261,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000290 DASWBF010000000
VERSION     DASWBF010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276261"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VF874_10525"
     CDS             <1..1008
                     /locus_tag="VF874_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX9176490.1"
                     /translation="ILTQGHYDHVGGTDLLSDPGTDIVMQANWESWRRDNDLLATFRA
                     ANASFAWADAISAALTFAKGRGRLPAQSSPVPTIIVDDELTLRIGERELVLRATPGGE
                     TTDSLVIWLPDDEVCLCGNVFGALFGHIPNLVTMRGDRYRDALTVIETIETVRELSPS
                     VLLTGHFDPISGKDRIRDELTRLRDAVAYLHDETVAGMNAGKDVYTLMREVTLPPALQ
                     VGEGYGMVRWNIRAIWENYAGWFHHRSTTELYAVAPEDFSNDIVALAGADAIVGRARK
                     ALADGEPLRAIRLTEIVRHVEPHHDGAKHVLRAAHEQLLEASTNFWESAWLRKQIAEL
                     T"
     gene            1005..1886
                     /locus_tag="VF874_10530"
     CDS             1005..1886
                     /locus_tag="VF874_10530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9176491.1"
                     /translation="MKYAVGAPVAARVTADPSWMSEFARHVEACGFDEIDAVEHTVLA
                     TAYDSVYPYDASGRVELPADCPVPDPLDLLAFIAGHTTTLGLATGVLVLPNHHPVVLA
                     KRVATVDALSGGRLRLCVGVGWLREEIEACGAPFGSRGRRADEQLEVLRLLWADNPDG
                     ATHSGEFFQFRNVACYPKPAQGERLPVHIGGHSMAAARRAGRLGDGLQPLRVTGPALA
                     SLIDVMRAEAVRAGRDPEALQLSLGHLVTKVDRDRAEKLAQLGADRIVLAMPAIDDLQ
                     QATDALSACAQRLGLAS"
     gene            1883..2344
                     /locus_tag="VF874_10535"
     CDS             1883..2344
                     /locus_tag="VF874_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9176492.1"
                     /translation="MTLDAETWLALSDLVHRYAAGVDDRRFEEVVDLFAAEATLAVPD
                     PPRTLTPTVVHQGHAEIRQAIEAVAAAARTQHAIVGEVYAAENNAARGRVSCIAHHWT
                     VRDEKVTDIVWHLRYDDEYIRTDAGWRIARRRLTIDAIETRPVRRLRPDES"
     gene            2351..>2525
                     /locus_tag="VF874_10540"
     CDS             2351..>2525
                     /locus_tag="VF874_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9176493.1"
                     /translation="MRCWVVWSVGLLAYIVAVLDRTTLGVSGLEAANRFGASPGVLSS
                     FVVLQVVVYAGAQV"
BASE COUNT          417 a          861 c          848 g          399 t
ORIGIN      
        1 atcctcaccc agggccacta cgaccacgtc ggcggcaccg atctgctcag cgacccgggc
       61 accgacattg tgatgcaagc gaactgggag tcgtggcggc gggacaatga cctgctcgcg
      121 acgttccgcg ccgcgaacgc atcctttgcc tgggcggacg cgatttcggc ggcgttgacc
      181 ttcgccaagg gacgggggcg tctgcccgcg cagagcagcc cggtgccgac gattatcgtc
      241 gacgacgaac tgaccctgcg catcggtgag cgcgaactcg tgctgcgcgc cacccccggc
      301 ggtgagacga ccgacagcct cgtcatctgg ctgcccgacg acgaggtgtg tttgtgcggc
      361 aacgtgttcg gcgcattgtt cggtcacatc ccgaacctgg tgacgatgcg gggtgaccgc
      421 taccgtgacg cgctcacggt gatcgagacc atcgaaaccg ttcgcgagct ttccccgtcg
      481 gtacttctga ccggtcactt cgacccgatc agcggaaagg accggatccg tgacgaactc
      541 acccggctgc gcgacgccgt cgcatacctg cacgatgaaa ccgtcgcggg catgaacgcc
      601 ggaaaggacg tctacacgct gatgcgcgag gtgacgctgc cgccagccct acaggtgggc
      661 gaaggctacg gcatggtgcg ctggaatata cgcgcgatct gggagaacta cgccgggtgg
      721 tttcaccacc gctccaccac cgagctgtat gccgttgcgc ctgaagactt ctcaaatgac
      781 atcgtcgcgc tggccggcgc cgacgcgatc gtcggccgtg cgcgcaaggc cctggccgac
      841 ggcgagcccc tgcgcgcgat acgactgacc gaaatcgtcc gccacgtcga gcctcaccac
      901 gacggcgcga agcacgtatt gcgcgcggcg cacgaacagc tcctcgaagc cagcacgaac
      961 ttctgggaat cggcctggct gcgcaaacag atcgcggagc tgacgtgaag tacgccgtcg
     1021 gtgccccggt cgccgcgcgg gttactgccg acccgtcgtg gatgtcggaa ttcgcccgtc
     1081 atgtcgaggc gtgcggcttc gacgagatcg acgccgtcga acacaccgtg ctcgcgacgg
     1141 catacgacag cgtctatccc tacgacgcct ccggccgcgt cgagctgccg gccgactgcc
     1201 cagtgccgga tccgcttgat ctgcttgcgt ttatcgccgg gcacaccacc acgctgggct
     1261 tggccaccgg tgtgttggtg ctgccgaacc accaccccgt ggtgctggcg aagcgggtgg
     1321 cgacggtgga cgcgctgtcg ggcgggcggc tgcggctgtg cgtcggggtg ggctggctgc
     1381 gcgaggaaat cgaggcgtgc ggggccccgt tcggcagccg aggccgacgg gccgacgagc
     1441 agctggaggt gctgcggctg ctctgggcgg acaaccccga cggcgcgacg cacagcggcg
     1501 agttcttcca gttccgcaac gtcgcctgct atccgaagcc tgcgcagggc gaacgactgc
     1561 ccgtccacat cggcggtcac agcatggcgg ccgcgcggcg cgcgggcagg ctcggcgatg
     1621 ggctgcaacc gctccgcgtg accggacctg cgctggcatc actgatcgat gtcatgcgcg
     1681 ccgaagcagt ccgcgccggc cgcgaccccg aggcgcttca gctttctctc ggccatctcg
     1741 tcaccaaggt cgaccgtgac cgagccgaga aactcgcgca actcggcgcc gatcgcatcg
     1801 ttctcgccat gccggccatc gacgacctcc aacaagccac ggacgcgctg tccgcctgcg
     1861 cccagcgact cgggctggcg tcatgaccct tgatgctgaa acatggctcg cattaagcga
     1921 tctcgtccac cggtatgccg ccggcgtgga cgaccgccga ttcgaagagg tcgtggacct
     1981 gttcgcagcg gaagcgacgc tggcggtgcc ggatccgccg cggacgctca ctccgacagt
     2041 cgtccaccag ggccacgcag aaattcggca ggccatcgaa gcggtcgccg cggccgcgag
     2101 aacccagcat gcgatcgtcg gcgaggtcta tgcagccgaa aacaacgcgg cccgtggacg
     2161 agtctcctgc atcgcgcacc actggactgt ccgcgacgag aaggtgaccg atatcgtgtg
     2221 gcatctgcga tacgacgacg agtacatccg caccgatgcc ggatggcgga tcgcccgccg
     2281 caggctgacc atcgacgcga tcgagacacg gccggtgcgg cggttgcggc cggacgagtc
     2341 gtaaccgagc atgcgctgct gggtcgtctg gtccgtcggg ctgctcgcct acatcgtcgc
     2401 cgtcctcgac cgcaccacgc tgggcgtgtc cggcctggag gccgcgaacc ggttcggggc
     2461 cagcccgggc gtgctgtcgt cgttcgtcgt gctgcaagtc gtggtgtacg cgggcgcgca
     2521 ggttc
//
LOCUS       DASWBF010000291         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_155732,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000291 DASWBF010000000
VERSION     DASWBF010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155732"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VF874_10545"
     CDS             complement(<1..835)
                     /locus_tag="VF874_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX9176494.1"
                     /translation="MTVPIFADVDTGVDDAIALVYLLAGAEARLVGIASTGGNVAVQQ
                     VCVNNLGLLELCRAGDIPVSKGAEQPLSCALRTAEDTHGPQGIGYAELPPTARRLTDY
                     DATEAWVRAAHAHPGELTGLVTGPMTNLALALRAEPKLPGLLRRLVVMGGSFEYRGNT
                     DVVAEWNISVDPEAAAEVFAAWSGARHLPIVLGLNLTENIVMTPAIFARLAAAAGASS
                     VASNPLIRVLEDAMRFYFEFHETQGEGYLAHLHDPLAAAVALNPSLVQTRTATVDVEL
                     TG"
     gene            complement(840..>2648)
                     /locus_tag="VF874_10550"
     CDS             complement(840..>2648)
                     /locus_tag="VF874_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9176495.1"
                     /translation="ETLTGLAGVEHVAHCGAIYDIAAPERAQHAANVDGTRSVIALAK
                     RLDATLHHVSSIAVAGDFPGEYTEADFDVDQNLPTAYHRTKFEAEQLVRLEPGLRYRV
                     YRPGAVVGDSCTGEMDKIDGPYYLFGLLATLAKLPSFTPIMLPDTGRTNIVPVDYVVD
                     ALVFLMHVPDRDGQTFHLTSPETIGLQGIYRGVAKAAGLPPMIGGLPRTLATPVLKLR
                     GRAKVIRNVAVTQLGIPAELLDVADLPTTFNADATREALRGSGIDVPEFAEYAPRLWR
                     YWAEHLDPDRARRDDPDGPLVGKHVVITGASSGIGRASAIAVAARGATVFALARNGKA
                     LDELVAEIRESGGTAYAFTCDVTDSSSVEHTVKDILGGFGHVDYLVNSAGRSIRRSVV
                     NSTDRLHDYERVMAVNYFGAVRMVLALLPHWRERRFGHVVNVSSAGTQARNPKYSAYI
                     PSKAALDAFSDVIATETLSDHITFTNVHMPLVRTPMIAPSRKLNPQRPISPEHAAAMV
                     VRALIEKPARLDHPLGTIAGAGNYFTPKIARRVLHQIYLGYPDSPAAQGVSPAEAASP
                     PRESPRRPKPPARAVRKLRVPRPVRRAARWVPGVHW"
BASE COUNT          432 a          871 c          902 g          443 t
ORIGIN      
        1 tacccgtcaa ctcgacatcg actgtggccg ttcgggtttg cacgagtgac gggttgagcg
       61 cgaccgccgc cgcaagcggg tcgtgcaggt gcgccaggta gccctcaccc tgcgtctcgt
      121 gaaactcgaa atagaaacgc atcgcgtcct cgagcacccg gatcagcggg ttggacgcca
      181 ccgacgacgc cccagcggct gcggccagcc gggcgaagat cgccggcgtc atcacgatgt
      241 tctcggtcag gttcagtccc aacacaattg gtagatgccg ggcgccggac caggcggcga
      301 acacctcggc ggccgcctcc gggtccacgc tgatgttcca ctccgccacc acgtcggtgt
      361 tgccgcgata ctcgaacgag ccgcccatga ccaccagccg ccgcagcagg cccggcaatt
      421 tcggctcggc acgcagcgcc agcgccaggt ttgtcatcgg gccggtgacc agtccggtca
      481 actcaccggg atgagcgtgt gccgcgcgca cccacgcctc ggtggcgtcg taatcggtga
      541 gccggcgcgc ggtcggtggc aactcggcgt acccgatgcc ttgcgggccg tgggtgtcct
      601 ccgccgtgcg aagagcgcaa ctcagcggct gctcggcgcc cttggacacc gggatgtcgc
      661 ccgccctgca caactcgagc agcccgaggt tgttgacgca gacctgttgt accgcaacgt
      721 ttccgcctgt cgaggcgatg cccaccaatc gtgcttcggc gccggcgagc aggtaaacca
      781 gggctatcgc gtcgtcgacg ccggtgtcga cgtcggcgaa gatcggcacc gtcacaaggt
      841 caccaatgaa cgcccggcac ccatcgcgcc gcacgcctca ccggccgagg cacccgcaat
      901 ttgcgcaccg cgcgggcggg cggctttggt cgccgtggcg actcgcgggg tggcgaagct
      961 gcctcggcgg gagaaacgcc ctgcgccgct ggggaatcgg gatatccgag atagatctga
     1021 tgaagaaccc ggcgcgcaat cttcggcgtg aagtagttgc ccgccccggc gatcgtgccg
     1081 agtgggtggt cgaggcgggc cggtttctcg atcagcgcgc gcaccaccat cgccgcggcg
     1141 tgctcggggc tgatcggtcg ttgcgggttg agctttcgtg acggcgcgat catcggtgtg
     1201 cgcaccagcg gcatgtggac attcgtgaat gtgatgtggt cggaaagtgt ttcggtggcg
     1261 atcacatcgg agaacgcatc caacgccgcc ttgctcggta tgtacgcgct gtacttcggg
     1321 ttgcgcgcct gcgtgccggc actggaaacg ttgacgacat ggccgaaacg tcgctcgcgc
     1381 caatgcggca gcaacgcgag caccatgcga accgcgccga agtagttgac ggccatcacg
     1441 cgttcgtagt cgtgcagtcg gtcggtggag ttgaccaccg accgacggat cgacctgccc
     1501 gcgctgttga ccaggtagtc gacgtgaccg aacccgccga ggatgtcctt cacggtgtgc
     1561 tctaccgacg acgaatcggt gacgtcgcac gtgaaggcgt aagcagtacc gccggattcg
     1621 cggatctcag cgacgagctc gtcgagagcc ttgccattgc gcgccagcgc gaacaccgtc
     1681 gcacctcgtg ccgcaaccgc gatcgccgac gcgcggccga ttccgctgga ggctccggtg
     1741 atcacgacgt gcttgccgac cagcgggccg tcggggtcgt cgcgacgggc ccggtcggga
     1801 tcgagatgct cggcccagta ccgccacagc cgcggcgcgt actctgcgaa ctccgggaca
     1861 tcgattccgc tgccgcgcaa cgcctctcgt gtcgcgtcgg cgttgaacgt ggtcggcagg
     1921 tcggcaacgt cgagaagttc ggcggggatc ccgagctgcg tgacggccac attccggatc
     1981 accttcgccc ggccgcgtag cttcagcact ggcgtcgcga gcgtccgcgg caggccgccg
     2041 atcatcggtg gtagccccgc ggccttggcg acgccacggt aaatgccctg cagtccaatg
     2101 gtttccggtg atgtgagatg aaaggtctgg ccgtcccgat ccgggacgtg catgagaaag
     2161 accagtgcgt cgaccacata atcgactggg acgatgttgg tgcggccggt gtcgggcagc
     2221 atgatcggcg tgaacgacgg cagtttcgcc agtgtggcga gcagcccgaa gaggtagtag
     2281 ggcccgtcga tcttgtccat ttcgccggtg cacgaatcgc cgacgaccgc gccgggccgg
     2341 tagacgcggt agcgcagtcc gggctccaaa cgcaccagct gttcggcctc gaacttggtc
     2401 cggtggtacg ccgtgggcag gttctggtcg acgtcgaagt ccgcctcggt gtactcgccg
     2461 gggaaatcgc cggcgaccgc gatcgacgac acgtggtgca acgtcgcgtc gagacgcttc
     2521 gcgagcgcga tcaccgatcg ggtgccgtcc acgttggcgg catgttgggc ccgctcgggc
     2581 gcggcgatgt cgtagatcgc gccgcagtgc gccacgtgtt cgacaccggc caggcccgtc
     2641 agcgtctc
//
LOCUS       DASWBF010000292         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1216104,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000292 DASWBF010000000
VERSION     DASWBF010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216104"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1554
                     /locus_tag="VF874_10555"
                     /pseudo
     CDS             1..1554
                     /locus_tag="VF874_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884926.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
BASE COUNT          236 a          505 c          539 g          274 t
ORIGIN      
        1 cagctggccg atgagttccc cgacggggtc tgggttttcg aactggccgc ggtggccgat
       61 ccggcggcgg tgcccgatgc agtcgcggcg gtgctgggca tcacccagca gccgggcaag
      121 agcgcaagcg acagcgtggc cgccgcgctg gagggcagga tccggttact ggtgatcgac
      181 aactgcgagc acgtgcgcga cgccgccgcg gatctgatcg aggccatcct ggcgcactcg
      241 gcgacggtga ggattttggc caccagccgc gaaggattgg gggtcgccga cgagcaactg
      301 tggctggtgc cctcgctgga tgtcggcgcg ggcatcgact ccgccgcggt ggaactgttt
      361 gttgaacgcg cccgtagcgt ggcctcgcgc ttctcgatgg gcagcagcga cgaggcggcc
      421 gcggtggtgg agatctgtcg ccgcctggat gggatcccgt tggccatcga gctggcggct
      481 tcacgcatgg catcgatgac cgccagcgag gtgcgggatc gtctcgatgt cgattccgcc
      541 tgctggtcgg gtcccggcgt ggcctggcac gccaccagac gctgcgccac gcggtggcgt
      601 ggtcctacaa ccacctcgat gacgcggaga agacactgct ggaccgttgt tcggttttcg
      661 ccggcggatt cgacctccac agcgcgtgcg cggtagcagg atccgatgct gtcgacgact
      721 tcgcggtgct ggatctgctg gacgcgctgg tgcgcaagtc gctgctggtc gccgaccggt
      781 cctccgggcg gacccggttc tcgatgctgg agacgatccg ccagttcgcc gaggaacaac
      841 tcgccgccgc cggggcggcg gaagaggttc gggcggcgca tgcccgccac tttgccggcc
      901 gcgaagcaga catccttgcg ctgtgggaca gcccacggca gcggggggcg tacgactggt
      961 tcaccgccga attggccaat ctgcgcaccg ccttccgctg ggccgccgac caacgcaacc
     1021 tcgatgtcgc cgccactatt gcgacgtacg cgggtttatt tggagtaatg gtcggaaatt
     1081 tcgaacccgt tgcctgggcc gaagaactca tcgagcctgc ccgcaccgtc aatcatccgc
     1141 ggctgacgtc cctgtgtgcg atcgcgtctc agtgcttcac gatcggacgg atcgaggagg
     1201 ctgtcggcta cagcgatgcc ggccggaaag cgatgcgcag tggccgcgac gaactgccgt
     1261 tcggcctcga ggtctggctc ggcaccgcgt acatgggcgt cggccaggcc gaactgttcg
     1321 ttgagttttg ccgcgcccag ctagcgtgcg gtcgcgacac tcataccctc accagggcat
     1381 acctggtcat ggcactggcg atcgtcgggt ctaatgagga ggcaacgtcc gccgcaaacg
     1441 gtctgatcga cgccgcggag gccaccgaca acccttatgc gttgtcctac gcgctgctcg
     1501 cctacggcct cgcctgccgc gacgccgatc ccaaccgtgc gcgtgatgct ctgc
//
LOCUS       DASWBF010000293         4333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1276380,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000293 DASWBF010000000
VERSION     DASWBF010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276380"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF874_10560"
     CDS             complement(<1..357)
                     /locus_tag="VF874_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX9176496.1"
                     /translation="MSSIAWDEAVIAFKALADQADEPAELWSTPQRLAWLDNVETLAR
                     ILPALRHQPINDLLAQATTQQVGGSLQQVLADRLRIYRRDAARRIEEAADLGARTTLI
                     GEPLPPKLEHTAAGQRA"
     gene            525..931
                     /locus_tag="VF874_10565"
                     /pseudo
     CDS             525..931
                     /locus_tag="VF874_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
     gene            950..1933
                     /locus_tag="VF874_10570"
     CDS             950..1933
                     /locus_tag="VF874_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9176497.1"
                     /translation="MSNLDTAPVEETCSASPQPGIEVLAARDVPLGGPRAMRVRRTLP
                     QRQRSLVGAWCFVDHYGPQDVRRGPGMVVPPHPHTGLQTVSWLFDGEIEHRDSGGVHA
                     SVHPGELNLMTAGAGICHSEVSTAATSALHGVQLWVALPESDRDTRRGFNHFVPQLVS
                     LDSTNLHVFLGELHGVRSPVHTFTPLFGAQLDLDPNVETVLDIDPHFEHGVLLDLGDV
                     EVAGVPVDVGELACQSAGPSTLNIRNTGSSPARVMLLGGTPFTEDLVMWWNFVGRSHD
                     DIASYRELWESEHDRFGAVEGYVGTPARLPAPPLPTTRLRPRPAPGRKDER"
     gene            1930..2265
                     /locus_tag="VF874_10575"
     CDS             1930..2265
                     /locus_tag="VF874_10575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9176498.1"
                     /translation="MTTDTTSAETTVTQETDRFTISVEGRPVGFTEFADRDGQRVFPH
                     TEVDDEFEGCGLATIVVAEALQATKDDGLRIVPVCKLVAAYVEKHSEFSGVVDPPTPD
                     IEEWLRTAS"
     gene            3009..4091
                     /locus_tag="VF874_10580"
     CDS             3009..4091
                     /locus_tag="VF874_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176499.1"
                     /translation="MSPLIVKGQQTDQTIAEASRSGAVVLNSTSEAATELTALLPTVT
                     TETAVDGVVVGHSTLTIPCGPGYDVPADWYFPTGPDPPQGVIWLQHGFPAQGPWYSTT
                     AATLAKETNSIVVVPSITSNFLQCDGCWLNGEPMERAVASMFVDRTALTASASHAAGH
                     PVTLPEKFVLVGHSAGGGFAVAVAGFIAGTPAIADLAGVVMFDGVALKDNMTTSLAKL
                     PDTLPVYQIAAPSYVWNNFGSGTAQLVQARPGQFTGFQLVGGSHVDSMRGVPIIEFAA
                     QLLTGFSRPANVQAVQTLAVGWINDMYAGVRHDGIYADPGQTIKIGGATAIALPTPPT
                     WFTPIETLIRNFVLAVERILFNLIAA"
     gene            4120..>4333
                     /locus_tag="VF874_10585"
     CDS             4120..>4333
                     /locus_tag="VF874_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9176500.1"
                     /translation="MVTPDAFFNRDGEAFVPTPLAQGPWGSTVSGNRVGGILAWAIER
                     EADDHELQPARLTVDLLRATAMAPVRV"
BASE COUNT          766 a         1508 c         1340 g          719 t
ORIGIN      
        1 ggcccgctgc cccgcggcgg tgtgctccag cttggggggc agcggttcgc cgatgagagt
       61 ggtgcgcgcc cccagatcgg cggcctcctc gatgcgccgg gccgcatccc ggcgatagat
      121 ccgcagccga tcggccagca cctgctgaag cgaaccgccc acctgctgcg tggtggcctg
      181 cgcgagcagg tcattgatcg gctgatgccg cagcgcgggc aggatgcgcg ccagcgtctc
      241 gacgttgtcc agccacgcca gccgctgcgg tgtgctccac aactcggcgg gctcatcggc
      301 ctggtcagcc agcgccttga acgcgatcac cgcctcatcc cacgcgatcg aactcatgtt
      361 cgaatactat cgcggccggc gacaacatct gacgccaaca ccagacccac caagccacct
      421 ggcgattcac ccaagttatc cacaaccgcc aattcatcca cagcctcatg tggtcgagcg
      481 gtcacccagc cgacatgtca gaccctgtcc ataccgttcg aggcatgact aattggatta
      541 acaccgtcag ccgcgaccac gtagaacaag gtgtccgcgg gcgcttcacg caagccaatc
      601 acggcaagcc atacatgctg cgcggatggc taagggtgac tgggtcgtct tctactcgcc
      661 caagaccgca ctcgacggcg gcgaaccgct tcaggcgttc accgccatcg ggcagatcgt
      721 cgacgaggag ccgtaccagg ccgagacaac aaccgatgcc tggcgcagaa atgtcgactt
      781 cctgcccgtg actgaaacgc cgatccgccc gctgatcgac gacttgaact tcatcgaaga
      841 caagaagcga tggggctaca agttcaggtt cggagtgttc aagatcgacg accatgacct
      901 cgagctcatc cgggaggcca taaccgggtg acggcgtacc gtcgcgacgg tgagcaacct
      961 ggatactgcg ccggtcgaag agacctgttc ggcatctccc cagcccggta tcgaggtgct
     1021 cgccgcccgt gacgtgccgt tgggtgggcc gcgcgcgatg cgtgttcgca ggacactccc
     1081 gcaacggcag cgctcgcttg tcggcgcatg gtgcttcgtc gaccactacg gaccgcagga
     1141 cgttcgtcgt ggcccgggga tggtcgtgcc accgcacccg cacaccggat tgcaaacggt
     1201 gagttggctt ttcgatggcg agattgagca tcgcgacagt ggcggcgtgc atgcttctgt
     1261 tcatcccggt gagctgaacc tgatgacggc cggggcgggc atctgtcact ccgaggtgtc
     1321 gacggccgcg acgtccgcgc tgcatggcgt acaactttgg gtggccctgc cagagtcaga
     1381 tcgtgacacc agacgaggct tcaatcactt tgtgccacag ctagtttcac tggatagcac
     1441 caacctgcac gtcttcctcg gcgagctaca cggtgtccgg tcgccagtgc acaccttcac
     1501 tccgctgttc ggtgcccagc tcgacctaga tcccaatgtc gagaccgtgc tcgacatcga
     1561 cccgcatttc gaacacggag tgctgctcga cctgggcgac gtcgaggtcg ccggcgtacc
     1621 ggtcgacgtc ggtgagctcg catgccagag cgccggcccg agcacactaa acatcaggaa
     1681 caccgggtcg tcgcccgccc gggtcatgtt gttgggcggt acgcccttca ctgaggatct
     1741 ggtgatgtgg tggaacttcg tcggccgcag ccatgacgac atcgcttcct accgcgaatt
     1801 gtgggaatcc gaacacgacc gcttcggcgc ggtagagggc tacgtcggca cgcccgcgcg
     1861 gctgcccgcc cctcccctgc cgaccacacg actgaggccg cgccccgccc cgggcagaaa
     1921 ggacgagcga tgaccaccga cacgaccagt gccgaaacca ccgtgaccca ggaaaccgac
     1981 cgcttcacga tcagtgtcga ggggcggccc gtcgggttca cggaattcgc cgaccgtgac
     2041 ggccagcgcg tcttccccca caccgaggtt gacgacgagt tcgaaggatg cggcctggcg
     2101 acgatcgtgg tggccgaggc gttgcaggcg accaaagacg acggtcttcg gatcgtgccg
     2161 gtgtgcaagc tcgtcgccgc gtacgtcgag aagcactcgg agttcagcgg cgtcgtggat
     2221 ccgccgacgc ccgacatcga ggagtggctc cgaaccgcga gctgagcgca gcgacgtcat
     2281 cggtgcgtca tatttccgaa cgtaagaacc cccgcgcgag gccgccgacc gcgataggtt
     2341 gagccgacct tcgtcgggga ggggggctcg tggcatcaca tcggcgtctg ctatggctcg
     2401 ccgtcgtgct cgcgggtttg ggcgcggcgc tgctattcgc caccggcacc gccaacgcca
     2461 gtacagggtc gacctctgcc gatggttcca gcaacccgac gtcatcagat tcgtcaacga
     2521 cgacgtccac accggacgac tccgaggcgt cgtcggaaga tccgggcgac accaccacac
     2581 cagcggcttc cacgatcagc gtggccatcc gcccgtcgtc gctgttgtca gccatcacgc
     2641 gtgccagaga cgctcgattg tcgacgacgc agccgcagtc aagcctgacg acttcaaggc
     2701 gcgtgcctta cacgacgcca agcgatgtgc cgcttcggcc tccttcggcc agggtcttcg
     2761 acatcgacac cgcgacgccg gcgctgtctt cgaccgcgcg gtcgaacgtg cgaacgaccc
     2821 tgctaaccct cgcgagcgac gccatcgaga agacagcgtc gctgcagtca gagccggcga
     2881 tgcagtcggc ttctctcaca ccggctgact ccccgccgca acagcccctc cgcactgccg
     2941 tcctcggcgt gctgtccagt ctcgggctct caccccgacc cgaccctcct gaggctgcgg
     3001 ccgctgtcgt gagtcccctg atcgtcaagg gtcagcagac cgaccagacg atcgccgagg
     3061 cgtcgcggtc cggggccgtg gttctgaact caacctccga ggcggccact gaactcaccg
     3121 cgttgctgcc gacggtcacc accgagacag cggtggacgg tgtcgtggtc ggccactcga
     3181 cactcacgat cccctgcggt cccggttatg acgttcccgc ggactggtac ttcccgacag
     3241 gtccggatcc gccgcaaggg gtgatctggc tgcagcacgg tttcccggcc caagggccgt
     3301 ggtacagcac gaccgcggcg acgctcgcga aggaaaccaa cagcatcgtt gtcgtaccct
     3361 cgatcacgtc gaatttcctt cagtgcgacg gctgttggct caacggcgag ccgatggaac
     3421 gcgcggtcgc gtcgatgttc gtcgaccgca cagcactcac cgccagcgcc agccacgcgg
     3481 ccggccatcc cgtcacgctg ccggagaagt tcgtcctggt cgggcactcc gccggcggcg
     3541 gattcgccgt cgccgtggcc ggcttcatcg ccggcacccc cgcaatcgca gacctagccg
     3601 gggtcgtcat gttcgacggc gtcgcgctca aagacaacat gacaacctcg ctggccaagc
     3661 tccccgacac cctgcccgtg taccagatcg cagcgccaag ctacgtgtgg aacaacttcg
     3721 gctccggcac cgcgcagttg gtgcaggcgc gtcccggcca gttcaccggc ttccagctag
     3781 tcggcggctc ccacgtcgac tcgatgcgcg gcgtcccgat catcgaattc gccgcacagc
     3841 tgctcaccgg cttctcccgt ccggcgaacg tgcaggccgt tcagacgctc gcggtggggt
     3901 ggatcaacga catgtacgcc ggcgttcgcc acgacggcat ctacgccgac ccgggacaga
     3961 cgatcaaaat cggcggcgcc accgccatcg cgctgccgac accgccgacg tggttcacgc
     4021 cgatcgagac gctgatccgc aacttcgtgc tggctgtgga acgcatcctg ttcaacctca
     4081 tcgccgcatg accgcaggcg cgcgttaacg tgacgcgcga tggtgacacc agacgcgttc
     4141 ttcaatcgcg acggcgaggc tttcgttccc acgccgttgg cccagggccc gtggggcagc
     4201 accgtcagcg gcaaccgggt cggcggcatt ctggcgtggg cgatagagcg cgaggcggac
     4261 gaccacgaac tacagcctgc tcgcctgacg gttgaccttc tgcgcgcgac ggccatggcg
     4321 ccggtccggg tgc
//
LOCUS       DASWBF010000294         3580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_31195, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000294 DASWBF010000000
VERSION     DASWBF010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31195"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VF874_10590"
     CDS             complement(<1..398)
                     /locus_tag="VF874_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010353442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX9176501.1"
                     /translation="MAGVADSAASRYTALRPHLDERQRRLLLGVEAQRLGRGGIKAIA
                     AATGVHPDTVARGVRELDGLPEPAARVHVPGGGRKKLTESDPELMSELKALVDPETRG
                     DPMSRLVWTTKSTRNLAGALTAAGHRVSDRT"
     gene            complement(469..1179)
                     /locus_tag="VF874_10595"
     CDS             complement(469..1179)
                     /locus_tag="VF874_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9176502.1"
                     /translation="MWDAAYVLGSLSPAERREFESHMGACKPCRQAVAEISGMPALLS
                     RLDSDDVAAIDEHGQSASAPPPPRPELLTSLLDKVRWRRRRSRVLTWAVGAAAAVVLV
                     AGVFVAWRSNPAVPAPQPAPQASSSALVMAPLTPTTLSATITLSSHQWGTQIDMNCTY
                     GPESENAAHEHEGEALDQLAMVVVGRDGSQSQLATWFSLTGVTASPGGSTAMSIDQIA
                     AVQVISVDDGNVILQRTL"
     gene            complement(1251..1760)
                     /locus_tag="VF874_10600"
     CDS             complement(1251..1760)
                     /locus_tag="VF874_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9176503.1"
                     /translation="MEAPETALMRVLYDEHAAALWRYAMRLTGDRARSEDVVQETLLR
                     AWQHPDVVGDGDRSARAWLFTVARNMIIDERRSARFRNEVGTLETAPEDTAPNEVDSA
                     LDRLLIGDAMAQLSADHRAVIRRSYYLGWSTAQIAADLDIAEGTVKSRLHYAIRALRL
                     TMQEMGVTR"
     gene            complement(1911..2714)
                     /gene="map"
                     /locus_tag="VF874_10605"
     CDS             complement(1911..2714)
                     /gene="map"
                     /locus_tag="VF874_10605"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX9176504.1"
                     /translation="MIALPGLRHRKVVPQRSAGELDAMAAAGALVAAALRAVQKAAQP
                     GVSTHDLDQIARSVIRDGGGTPSFLGYHDYPATICASVNDRVVHGIPSAAEVLVTGDL
                     VSIDCGAIVDGWHGDAAVTFGVGALIPVDEALSRATKESLEAGIAAMVPGNRLTDVSH
                     AIEEATHAAEARYDRSFGIVEGYGGHGIGRRMHMDPFLPNEGLPGRGPLLASGSVLAI
                     EPMLTLGTTKTTVLDDQWTVVTADGSRAAHWEHTVAATDDGPRILTAGR"
     gene            complement(2739..3284)
                     /locus_tag="VF874_10610"
     CDS             complement(2739..3284)
                     /locus_tag="VF874_10610"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX9176505.1"
                     /translation="MRVVLLGPPGAGKGTQAVKLAEKLGTPQISTGDLFRSNINDGTE
                     LGLEAKRYLDAGDLVPSELTNALVDKRLDEPDAADGFILDGYPRSVEQAEALTRMLAA
                     RDTKLDAVLEFRVSEQELLTRLKDRGRADDTEEVIHNRMKVYRDETAPLLEYYDGTVK
                     TVDAVGSMDEVFARALRALGR"
     gene            complement(3281..>3580)
                     /locus_tag="VF874_10615"
     CDS             complement(3281..>3580)
                     /locus_tag="VF874_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9176506.1"
                     /translation="DERADEMKKFGGFIPGIRPGRPTADYLRYVLNRITLPGSIYLGV
                     IAVLPNLFLEIGNTGGAQNLPFGGTAVLILIGVGLDTVKQIESQLMQRNYEGFLK"
BASE COUNT          543 a         1266 c         1158 g          613 t
ORIGIN      
        1 gtccggtcgg agacccgatg ccccgccgcg gtcagcgcgc cggccaggtt ccgcgtcgac
       61 ttcgtggtcc acaccaaccg cgacatcgga tcaccacgag tctccggatc caccaacgct
      121 ttcagctcgg acatcagctc cggatcggac tcggtcagct tcttgcggcc accgccgggc
      181 acatgcaccc gcgccgccgg ctccggcaac ccgtcgagct cccgcactcc ccgcgcgacg
      241 gtgtcgggat gaacgccggt cgccgcggcg atcgccttga ttccaccccg cccgagccgt
      301 tgggcctcca cccccaacag cagacgccgc tgccgctcat ccaggtgcgg gcgcagcgcc
      361 gtgtacctcg acgccgccga gtccgcgact cccgccatgc cggtcattat cacaaccgta
      421 gcgggcgcgg cagcgaaaac ctagattatt ttgccgcgcg tccttagctt agagcgtgcg
      481 ttgcagtatg acgtttccgt cgtccacgga aatcacttgc accgccgcga tctgatcgat
      541 cgacatggcg gtgctcccgc cgggtgacgc ggtcacaccg gtcagcgaaa accaggtggc
      601 cagttggctc tggctaccgt cgcggccgac gaccaccatc gccagttggt ctagagcctc
      661 tccctcgtgc tcgtgcgctg cgttctccga ctccggcccg taggtgcagt tcatgtcgat
      721 ctgcgtaccc cattgatggc tgctgagggt gatcgttgcg ctcagcgtcg tcggtgtcag
      781 aggtgccatg accagcgccg acgaactcgc ctgcggcgct ggctgaggcg caggcacggc
      841 agggttggag cgccacgcga cgaacacccc ggcgaccagc accacggcgg ccgcggcgcc
      901 caccgcccag gtcagcaccc gcgaccggcg acgtcgccac ctcaccttgt ccagcagcga
      961 cgtcagcaat tcgggccgcg gcggcggagg tgcgctcgcc gactggccgt gttcgtcgat
     1021 cgcggcgacg tcgtcgctgt cgagccggga cagcagcgcg ggcattccgc tgatctcggc
     1081 gaccgcctgc cggcacggct tgcaggcgcc catgtgcgat tcgaactcgc gccgctccgc
     1141 cggcgacagt gagccgagca cgtatgcggc gtcccacatc gcgtattcgt gcgtgtcgac
     1201 ggccgcgccg tgcgggcggc cggcgggagt cgcacctgac cctggctggc tcatcgcgtc
     1261 acccccattt cctgcattgt cagccgcaat gcgcgtatcg catagtgcag ccgagatttc
     1321 acggtgccct cggcgatgtc caaatcggcc gcgatctgcg cggttgacca cccgagatag
     1381 tacgaccggc ggatcacagc gcggtgatcg gcggacagtt gggccatcgc gtcgccgatc
     1441 agcagccggt ccagcgccga gtcgacctcg ttgggcgcgg tgtcctcggg ggcggtctcc
     1501 agcgtgccga cctcgttgcg gaaccgtgcg cttcgtcgtt cgtcgatgat catgttgcgg
     1561 gcgaccgtga acagccacgc gcgcgccgag cgatcgccgt cgccgacgac gtcgggatgc
     1621 tgccaggccc gcagcagcgt ttcctgcacg acgtcctccg atcgggcccg gtcaccggtg
     1681 agccgcatcg catagcgcca caacgcagcg gcgtgctcgt cgtacagcac ccgcatcagc
     1741 gccgtttccg gagcctccat caccgacctc ccggtggtga cacggattaa ttgacccgat
     1801 ggttcatttc ggttgtcacg tcatctgctc ttcgggcaag cggctcatcg ctgccttctg
     1861 ctcttcgcgc aagcggctca tcgctgcctt ctgctcttcg cgcaagcggc tcatcgccca
     1921 gcggtcagga tgcgaggccc gtcgtcggtc gccgccacgg tgtgctccca gtgtgccgca
     1981 cgagagccgt ccgcggtgac gaccgtccac tggtcgtcga ggaccgtggt cttcgtcgtc
     2041 ccgagggtga gcatgggttc gatggccaga acagaacccg acgccagcaa cggtccgcga
     2101 cccggcaagc cttcgttcgg caggaacggg tccatgtgca tccgccggcc gataccgtgc
     2161 ccgccgtagc cttcgacgat cccgaacgac cggtcatagc gcgcctcggc ggcgtgggtg
     2221 gcctcctcga tcgcgtgcga gacgtcggtg agccggttac cgggcaccat cgccgcgatc
     2281 ccggcctcca gcgactcctt ggttgcccgc gacagcgcct cgtcgaccgg tatcagtgca
     2341 ccgacaccga acgtgaccgc ggcgtcgccg tgccaaccgt cgacgatcgc accgcagtcg
     2401 atcgacacca gatcgccggt caccagaact tcggcggccg atgggatgcc gtgcaccaca
     2461 cggtcgttga cggacgcgca gatcgtcgcc gggtagtcgt ggtagccgag gaacgacggc
     2521 gtgccgccgc cgtcgcgaat caccgaccgg gcgatctggt cgaggtcgtg cgtcgatacg
     2581 cccggctgag cggctttctg cacggcgcgc aacgccgctg ccaccagcgc cccggcggcg
     2641 gccatcgcgt cgagttcacc ggcgctgcgc tgcggaacca ccttgcggtg gcgcagcccg
     2701 ggcagggcga tcacgtcagc ggctccgtca aggacgacct atcggccgag ggcgcgcaat
     2761 gcgcgagcga atacctcgtc catcgacccg accgcgtcga cggtcttcac ggtgccgtcg
     2821 tagtactcca gcagcggagc cgtttcgtcg cggtacacct tcatccggtt gtggatgacc
     2881 tcttcggtgt cgtcggcgcg gccgcggtcc ttgagccgcg tgagcagttc ctgctcgctc
     2941 acccggaact ccagcactgc gtccagcttg gtgtcgcggg cggccagcat ccgggtcagc
     3001 gcctcggcct gctcaaccga gcgggggtag ccgtcgagga tgaagccgtc ggccgcatcg
     3061 ggttcgtcga gtcgcttgtc caccagcgcg ttggtcagct cagagggcac aaggtcgccg
     3121 gcgtcgagat agcgcttggc ctccaggccg agttccgtgc cgtcgttgat gttgctgcga
     3181 aagaggtcgc cggtggagat ctgcggggtt ccgagcttct cggccagctt caccgcctgc
     3241 gtgcctttac cggcgcctgg cgggccgagc agaacgactc tcacttcagg aacccttcat
     3301 agttgcgctg catgagctgg gactcgatct gtttgaccgt atccaatccc acgccgatca
     3361 gaatcagaac cgcagtgccg ccgaacggca agttctgggc accgccggtg ttgccgatct
     3421 caaggaaaag gttcggcaag accgcgatga cgccgaggta gatcgaaccg ggcagggtga
     3481 tccggttcag cacatagcgc aggtagtcgg cagtcgggcg gcccggccgg atgccgggga
     3541 tgaacccgcc gaacttcttc atctcgtcgg cccgctcgtc
//
LOCUS       DASWBF010000295         8450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_688186,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000295 DASWBF010000000
VERSION     DASWBF010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688186"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VF874_10620"
     CDS             complement(<1..181)
                     /locus_tag="VF874_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9176507.1"
                     /translation="MTNLTLWARPAWSLDNWVNDFLGPATANDWSFKPAAEVVKDGED
                     AVIRVELPGIDVEKDV"
     gene            complement(290..1069)
                     /locus_tag="VF874_10625"
     CDS             complement(290..1069)
                     /locus_tag="VF874_10625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9176508.1"
                     /translation="MTSPQTFRPDPEMFDKLYRGEPTFEGAPPPTGIPWDVGQAQPRL
                     MELEALGAISGEVLDIGCGLGDNAIFLASRGHSVTALDGSAAAIERARRRAAEAGVRV
                     TFDVADATNLTGYDGRFDTVVDSALYHCLDDDGRQSYAAGLHRATKPGARWHLFCFSD
                     GNVNGVIAPVGSVPEENIRDTLTANGWRIDFLGPTTYLANTSSMAGDPNNLPEQMRQQ
                     MSPEQVELMKKTRERFAAIVPLLDDDRMHLPFTVVHAVRVD"
     gene            complement(1089..1634)
                     /locus_tag="VF874_10630"
     CDS             complement(1089..1634)
                     /locus_tag="VF874_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9176509.1"
                     /translation="MRTQVHTARLIHTADLDGETLHSAHQLLVDVFDDDFSEDDWEHT
                     LGGMHALICDHGALIAHAAVVLRRLLYRGAPLRTGYVEGVAVREDCRGQGLGTALMDG
                     VEQVIRGAYRLGALSLSSEARQLYLDRGWIQWKGPTSVLAPRGMTRTPEDDGAVFVLP
                     VDAELDPTADLVCDWRSGDVW"
     gene            complement(1659..2536)
                     /locus_tag="VF874_10635"
                     /pseudo
     CDS             complement(1659..2536)
                     /locus_tag="VF874_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891754.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
     gene            complement(2533..3167)
                     /locus_tag="VF874_10640"
                     /pseudo
     CDS             complement(2533..3167)
                     /locus_tag="VF874_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
     gene            complement(3262..3972)
                     /locus_tag="VF874_10645"
     CDS             complement(3262..3972)
                     /locus_tag="VF874_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX9176510.1"
                     /translation="MTPDTRTHDEPATGYASVGSAFYPVLVGAFTGLVLISNITATKG
                     VAFGPVVGNWSLITDGGFIMFPLTYVIGDVLSEVYGFRATRRAIYVGFTMQAVAALAF
                     WVTIFLPAADFYANQEALRTVVGASTQLIVASLAGFVCGQTLNSWVVVRIKARTKEKH
                     LWARLIGSTIVGEFADTLVFCSIAAGAIGITTTRDFITYVVLGWIYKTAVEVVVLPVT
                     YRVIAYIKRHEPTYRPAV"
     gene            complement(3972..4940)
                     /locus_tag="VF874_10650"
     CDS             complement(3972..4940)
                     /locus_tag="VF874_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9176511.1"
                     /translation="MQTLSRRGFFGVAGAAGLLTIAACSSDKSADSSPSEVTVKHQFG
                     ETTIAAPPKRVVSAGFTEQDDLLAVGVVPIAVTNWWGEQPFAVWPWAQPKLGSAQPTV
                     LSLDNGLEFDKITALKPDLIVATNAGVDQDSYNRLSSIAPTIPQSADQPFFEPWKVQA
                     TAIGQAVFQTDQMTSLISDVDNKFTEVANANSAFKDKKALIVQGSLYTDNFVATLPGW
                     RTEFLTQMGFVVPDDIKQFGVDDHRALIPRDKAVDALRGADVLIWTTESDDEQARLSA
                     DPTFRKLNNRNVFTGKDLAGAIAFSSPLSLPVVATQLPPMLAKVIT"
     gene            complement(4988..6099)
                     /locus_tag="VF874_10655"
                     /pseudo
     CDS             complement(4988..6099)
                     /locus_tag="VF874_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241745.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
     gene            6376..6594
                     /locus_tag="VF874_10660"
     CDS             6376..6594
                     /locus_tag="VF874_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9176512.1"
                     /translation="MLHLDQVDADPLVLGADPAPGDDAWVVGYPRGEVRVTTGRVVDE
                     VDGTKYGVSGRVIRTRAEADPGNSGDRC"
     gene            complement(6577..7020)
                     /locus_tag="VF874_10665"
     CDS             complement(6577..7020)
                     /locus_tag="VF874_10665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9176513.1"
                     /translation="MRADVTNEADVAAVVSRAIDEFGHVDIMCNNAAAPGKDLWIWEQ
                     TLENWNATIAVDVTAAMLCTREVVKQSMLERRSGVILNFSSTAGYNGMVRKTHYVTAK
                     ASLRAFTKAVALEVGPHGIRCNCISNTRLRTQCPPLVRAGSAAVP"
     gene            complement(7070..7669)
                     /locus_tag="VF874_10670"
     CDS             complement(7070..7669)
                     /locus_tag="VF874_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HEX9176514.1"
                     /translation="MAAMLLLGFVTSLTLIVAIGAQNAFVLRQGIRGEHVLAVVALCT
                     VSDVVLIAAGVAGFGVLVAAHPEAITVARFGGAAFLVGYGILAAVRAARPPSGLTPSD
                     AAPARIAGVVLTCLALTFLNPHVYLDTVVLLGALANEQQDGRWLFGTGAVGASAVWFA
                     TLGYGARRLGPLFASASAWRALDGLISVMMLGLAAALLT"
BASE COUNT         1493 a         2710 c         2851 g         1396 t
ORIGIN      
        1 tgacgtcctt ctctacgtcg atgcctggca gttcgacgcg gatcaccgcg tcttcgccgt
       61 ccttgacgac ctcggccgcc ggcttgaacg accagtcgtt cgccgtagcg gggccgagga
      121 aatcgttcac ccaattgtcg agactccagg cgggacgcgc ccagagggtc aggttggtca
      181 tgtgtgtctc ctcgatgtgg ttggccggcc cgatgccgtc cttgctacca agtaaaactt
      241 gagtcgacca cgctaaagtt ccagcaggcg ttcgccctgg gcgaacccgt caatccaccc
      301 gtaccgcgtg tacgaccgtg aacgggaggt gcatgcggtc atcgtcgagc agcggcacga
      361 tcgcggcgaa ccgctcgcgg gttttcttca tcagttcgac ttgctccggg gacatctgct
      421 ggcgcatctg ctcaggcaga ttgttcggat ccccggccat cgagctggtg ttcgccaggt
      481 acgtggtcgg acccagaaag tcgatccgcc agccgttggc ggtcagggtg tcgcggatat
      541 tctcttccgg caccgagccc accggggcga tcacgccgtt gacgttcccg tcagagaagc
      601 agaacagatg ccagcgcgcg ccgggtttcg tcgcgcgatg caggcccgcg gcgtacgact
      661 gccggccgtc gtcgtcgagg cagtggtaca gcgcgctgtc gacgacagtg tcgaaccggc
      721 cgtcgtaacc ggtcaggtta gtggcgtcgg cgacgtcgaa cgtgacccgg acaccggcct
      781 cagcggctcg acggcgggcc cgctcgatcg cggccgccga cccatcgagt gccgtcaccg
      841 aatgtccacg ggaggcaaga aagatcgcgt tgtcaccgag cccgcagccg atgtcgagca
      901 cctcgccgga gatggcgccg agcgcttcca gttccatcaa ccgtggctgt gcctgcccga
      961 cgtcccacgg aatacccgta ggtggcggcg cgccctcgaa agtgggctca ccgcgataga
     1021 gcttgtcgaa catctcggga tcagggcgaa aggtctgtgg actcgtcacc tcaacgaacg
     1081 tacgcctctc accagacgtc gccagatcgc cagtcgcaca ccaaatctgc tgtcggatcg
     1141 agttccgcgt ccacaggtag gacgaacacg gctccgtcgt cctccggtgt ccgggtcatc
     1201 ccacgcggag ccagcaccga cgtcggaccc ttccactgga tccagcctcg gtcgaggtag
     1261 agctggcggg cttcggatga cagactgagc gcgccgagcc ggtaggcccc gcggatcact
     1321 tgctcgacac cgtccatcaa tgccgtgcca agaccttggc cgcggcagtc ctcccgcacc
     1381 gcgacgccct ccacgtaccc ggtgcgcagc ggcgccccgc gatacagcag tcggcgcagg
     1441 accacggcgg catgagcgat cagtgcgccg tggtcgcaga tcagcgcatg cataccgccg
     1501 agagtgtgct cccagtcgtc ctcgctgaaa tcgtcgtcga aaacgtcgac cagcagttga
     1561 tgtgcgctgt gcagggtctc gccgtcgaga tcggcggtgt ggatcaggcg ggccgtgtgt
     1621 acctgagtgc gcacatgttt gggtctacca cattcatgtc aggaccggcg gcgcggcttg
     1681 gaccagtgga agcggaccgt ctgcccgggc cggatctggg cgatacggtc gatgtcggcg
     1741 tcggtgacca cacctgcgac gggatacccg ccggtcaccg gatggtccgg gccgaggatc
     1801 actggtaacc cgttgggcgg cacctggatc gcgcccctgg tggccccctc gctggggagt
     1861 tgccgatccg gccagcggtg ctgcagcggt ttcccgacca gccgcatgcc cacgcggtcg
     1921 ctgcggtctg atgccaccca gtcggtgtgc accaacgcgt ccggatcggt cagccagtcg
     1981 tcccgcggcc ccgggaccac cgcgagttcg atcaggtcgt cggtgatcga ggcgacgggc
     2041 gcctggtcga gttcggggaa gtcggcggtg tggtcgccga ccggcagcac gttgccgggc
     2101 tgcagtggcg cggggcgagc gccgacatca cgtcatagct gcgcgacccg agcacgggcg
     2161 tcacgtcgat cccgccacgc accgcaaggt aggtacgcag gcccgagtgc ggagcaccca
     2221 gcgagatcac ctggctggca tgtgcgtagt gaatgctgtt gatcccgaac ggaattccgt
     2281 tgacggcggt gtcggtgtcg gcgccggtga cggcgatcgc gacgtcgccg ccccggaccc
     2341 gggccatcag gccgccgaac atcacctcga tcgtcgcgcg gtcgcccgga ttggcgacaa
     2401 gccggttggc cagcgtgtgg gagcgtcggt cggcggcgcc ggagcgtgtc acgccgatgt
     2461 gcgccagccc cggacggccg aggtcctgga ccaaggcaag cggcccggtt cgcatgattt
     2521 cgagggtggt cgtcatcggg actcccggaa ttggacggtc atgcccggcg tcagcagcgc
     2581 cggatcggcg cggtcgatct cccacagagt ggcatccgtg cgcccgatca gctgccagcc
     2641 gcccggcgcc tctcggggat atacgccgct gaattcgcct gcaagcgcaa ccgatccggc
     2701 gggcacgcgg gtccgcggct cgtcgcgtcg cggcacgtcg agccgcgggt ccccaccgac
     2761 gagacaggcg aacccgggga cgaatccgcc gaacgccacc cgccatgtcg ccccggtgtg
     2821 cgcggcgacg actcccgcgc gccgagtccg gtgatgcgac cgacctcgtc gagatccgct
     2881 ccgtcgtaga cgacatcgat cacgatgtcg gcgctgccgg gtgcgacgac gtcgtcgtcg
     2941 gggtcgaccc tcagcttctc gatgcgctgg cgcgtcgcgg gcaaatagcg cgcgccgtcg
     3001 acctcgacca tcaccgtgcg cgacgcgggc acgatgtcgg tgacgccgag gagatcggcc
     3061 tcccgaagcg cggccgtgaa ggcaagcacc tcagcggtac tctcacattc cagcaacagc
     3121 gcgtgatcgc cgtagtcgag caccctcaaa tccgttgcca cactcatcca cccaaggtat
     3181 gcccgaatgt ggcgtgtgac atcaaccctg agcgcactgc cagagctgcc ttagccaagt
     3241 ctcaaatccc gcttgcggtc gtcacaccgc cggccggtac gtcggctcgt gacgtttgat
     3301 gtacgcgatc acgcggtagg tgaccggcag cacgactacc tcgaccgcag tcttgtagat
     3361 ccagcccagg acgacgtagg tgatgaagtc acgcgtggtg gtgatgccga tggcgcccgc
     3421 cgcgatgctg cagaacacca gagtgtcggc gaactcgccg acgatcgtgg agccgatcag
     3481 gcgcgcccac aggtgctttt cttttgtgcg ggccttgatg cgtacgacga cccatgagtt
     3541 cagcgtctgc ccgcagacga acccggccag gctcgcgacg atcagttgcg tggacgcgcc
     3601 gacgacggtg cgcagcgcct cctggttggc gtagaagtcg gcggccggca gaaatatcgt
     3661 gacccaaaag gccagcgccg caacggcttg catcgtgaat ccgacgtaga tggcacggcg
     3721 ggtggcgcgg aacccgtaca cctcgctgag cacgtcgccg atcacgtagg tgagagggaa
     3781 catgatgaat ccgccgtcgg tgatgagcga ccagttgccg acgacgggtc cgaacgcgac
     3841 gcccttggtg gcggtgatgt tcgagatcag cacgagcccg gtgaacgcgc cgacgagcac
     3901 cggatagaag gccgaaccca ccgatgcgta gccggttgcg ggctcgtcgt gggtgcgggt
     3961 gtcgggggtc atcacgtgat tactttggcg agcatgggtg gcagctgggt ggcgacgacg
     4021 ggcaggctca acggcgacga gaacgcgatc gccccggcca ggtccttgcc ggtgaacacg
     4081 ttgcggttgt tgagcttccg gaaggtcggg tcggccgaga gccgcgcctg ctcgtcgtcg
     4141 ctctcggtgg tccagatcag cacatcggcg ccacggagcg cgtcgacggc cttgtcacgg
     4201 gggatcagcg cgcggtggtc gtcgacgccg aactgcttga tgtcgtcggg aaccacgaac
     4261 cccatctgcg tcagaaactc cgtgcgccaa ccgggcagcg tcgcgacgaa gttgtccgtg
     4321 taaaggctgc cctgcacgat gagcgccttc ttgtctttga acgcagagtt ggcgttggcg
     4381 acctcggtga acttgttgtc cacgtcgctg atcaacgacg tcatctggtc ggtctggaac
     4441 acggcctgcc cgatcgcggt ggcctgtacc ttccacggct cgaagaatgg ctggtctgcg
     4501 gattgcggga tcgtcggcgc gatgctcgac agccggttgt agctgtcctg gtccacgccg
     4561 gcgttcgtgg cgacgatcag gtcgggcttg agcgcggtga tcttgtcgaa ctcgaggccg
     4621 ttgtcgagcg acagcacggt cggctgagcc gaacccagct tgggctgcgc ccacggccac
     4681 accgcgaacg gctgctcgcc ccaccagttc gtgacggcaa tcggcacgac gccgacggcc
     4741 agcagatcgt cctgctcggt gaagccggcg ctgaccaccc gcttgggcgg ggcggcgatc
     4801 gtggtctcgc cgaactgatg cttgacggtc acctcggacg gcgagctgtc agccgatttg
     4861 tccgacgagc acgcggcgat cgtgagtagc cccgccgcgc cggccacccc gaaaaagccc
     4921 cgcctgctca gtgtttgcat ccgcgtgagc gtagctggcg accgcccgtg gacggcgcgg
     4981 aaacgcgcta accgacgtgg aagtcaccga acagcacgac cgccaaaccg agcgccaccg
     5041 cgatgttgac cacggtcgcc gaaccgaaga ccgcaatcgg ccgccagccg gcctcccgta
     5101 gacccttcaa cgagaactcg aggccgatcg acacgaacgc gaagatcaga aaccaggtcc
     5161 gcaagtcgtt gacgacggtg atctgctcct agccgccagc cacccactgc ggataaaacg
     5221 tgccgatcac cgaggcggcg atgaagccga gcacgaactt gggaaagcgt tcccagaact
     5281 gggcaccgtc ggccgttgcg cttgtgcgtc gggcgctacc cggaacgcga agtacgcggt
     5341 cagcgcgatc gccacgacac cgatgagtgc gttctgagtg gtcttgacga tcgtcgcgat
     5401 ctgcaaggcc tcttcgccgg cgatcgcacc ggacgcggcg accgccgccg tcgtgtcgat
     5461 gttgccgccg atccatgcgc cggcgacggc actggacggt cccagcacgt cggcgagcca
     5521 gggcagcaag aagatcgacg gcagcgcgaa cgcgatgacc agtgacgcgc tgtacgcgag
     5581 ctgctcgcgc ttggcctgca cggcgccggc ggcggcgatc gctgcgctga cgccgcagat
     5641 ggacacggcc gaggcgagca gggcgcgcag cttgtcgtcg attccgagcc ggccgccgag
     5701 ccaccaggtg aagccgaaca cgatcgagat cagcagcagc gcttggaggc tcgccggccc
     5761 cgcggcggtg acgagaactt tcaggttgat cgacgctccg agcagcacga ggccggtctt
     5821 gatgaagaac tcggtccgga aaccgttcga aagtgcctcg cgcacagcga gtttggtcag
     5881 gatgatgttg ccgatcagac ccagagcgat cgcatacacg gggaattcga tggatttcgc
     5941 caccctggcg agtggcgtgc cggcagccca gtgcgggacc tgcgtctcca agaaccgggt
     6001 cgccgcaccc aatgcgatga cgacgacgac gccgatgacg gcgtagggca gaccggacga
     6061 gcgctctacc ggttgcgctt cggtggtcgt ttccgtcacg ggatcacgct gccggggatg
     6121 gcaccgagaa gcacgagagc aagcagtgtc aggccggcga tgacggccaa ccagtcttcg
     6181 ttcacggcgg cacggtagcc cgccgacgaa cccgttcgac accgttgggc tcaggccgaa
     6241 cagaactgca gaagtttgcc gacgatgttt cacgcccgcc tgcggagggg aaaccgaacg
     6301 ggtgagcatc gagaccgccg acgaccgcac gctttcggtc gatgcggtct cggtcattcg
     6361 cttgtcgatg ctgccgtgct gcacctggac caggtggacg ccgatccact ggttctaggc
     6421 gccgacccgg ctcccggcga cgacgcatgg gtggtgggct atccacgggg agaggtgcgg
     6481 gtaacgaccg gtcgagtggt cgacgaagtc gatggcacca agtacggagt gtccggacgg
     6541 gtcatccgca cgcgggccga agctgatcca ggaaattcag gggaccgctg ctgaaccggc
     6601 gcggacaagt ggcgggcatt gtgttcgcag tcgagtattc gagatgcagt tgcagcggat
     6661 cccgtgcgga ccgacttcca gcgcaaccgc cttggtgaag gcgcgtaggg aggctttcgc
     6721 ggtgacgtag tgcgtcttgc ggaccatccc gttgtagccc gctgtcgagg agaagttcag
     6781 gatcacaccc gaacggcgtt ccagcatgga ctgtttcacg acctcgcggg tgcacaacat
     6841 ggcggcggtg acatcgacgg cgatggtggc gttccagttt tcgagtgtct gttcccagat
     6901 ccacagatcc tttccgggtg ctgcggcgtt gttgcacatg atgtcgacat ggccgaactc
     6961 gtcgatcgcc cggctgacca ccgccgccac gtcggcttcg ttggtgacgt cggcgcgcat
     7021 ggcgatgtct tcagcgcggg cgatcagtgg cccctcgctg cgggccggac tatgtcaaga
     7081 gtgcggcggc gagtcccagc atcatcaccg agataagccc gtcgagggcg cgccaggccg
     7141 acgccgatgc gaacagcggc ccgagtcggc gcgcgccgta tcccagcgtg gcgaaccaca
     7201 cggcgctggc gccaaccgcc ccggtaccga aaagccaacg gccgtcctgt tgttcgttcg
     7261 ccaacgcgcc cagcagcacg acggtgtcca gatagacgtg cgggttgagg aacgtcaaag
     7321 cgaggcaggt gagaacgacg cccgcgatcc gtgccggcgc ggcatccgag ggcgttaggc
     7381 cggacggcgg ccgtgcggcg cgcaccgcgg cgaggatgcc gtatccgacc aggaacgccg
     7441 ccccgccgaa ccgtgcgacg gtgatcgcct ccgggtgggc ggcgaccagc acgccgaagc
     7501 cggcgacacc ggcggcgatc agcaccacat ccgacacggt gcacagcgct acgacggcaa
     7561 gcacgtgctc accgcggatg ccctggcgca gcacgaaggc gttctgggcg ccgatcgcga
     7621 cgatcagcgt caggctcgtg acgaagccga ggagcagcat ggcggccatc tccgcgacac
     7681 taggaaccga tgctgcagaa gtacagctaa ttattcttcc gatgcattag cgtcgctaat
     7741 gtgaatctgg acggccagca gctcgctgca ttcgtggctg taggttctca ttctccgtgt
     7801 cggaattctc atcttcgtgt cggatgaatg tcggatccga catcaagatg agaaggtgta
     7861 tagtatagct atacatttcg gtcggtgaag cgctcgcgga ggcgtgtgcg taatccgagg
     7921 gcgggtaggt gcagccgcga tccgagatcc gagaacctag agctactaga gagccaagct
     7981 gtgcctcggc gatgacgcct ccaatcgctt agtggccgag gccgatgggc actggcggga
     8041 gatcgccgag gaactactta gcttagctta gggccgtagc tgcgggtcta tcggctctcc
     8101 tctgcgcgat cgcttaccgg atgagcctca cggcctcgga tggattgtcc ggctgcggac
     8161 aacgaccgga gcgaggcaga atcacgagcc tgctcgccgg aatctgctgc tgcagaatgt
     8221 gactcgagtt cacagggatg atgcggtcat gactcccgtg gacgacgagg gtggggcagc
     8281 tgacccgcac gtgggtgttc ggccgccgcc ctacccacgt acatcgagcg aggcactgcg
     8341 cgggatgagg tcgcgcggaa gtttagcggg acttaatcct ctatcggtta gctgagaagg
     8401 cgcctagcgt tgccagcgtg gtagcagccg agagcacgta catgcgtgca
//
LOCUS       DASWBF010000296        14124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1216381,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000296 DASWBF010000000
VERSION     DASWBF010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14124
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216381"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1187)
                     /locus_tag="VF874_10675"
     CDS             complement(45..1187)
                     /locus_tag="VF874_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9176515.1"
                     /translation="MNDVAIIGVGLHPFGRFDKTAMQMGAEAIRGALDDAGLEWKDIQ
                     FGVGGSYEVSNPDAVTRLVGLTGIPFTDVFNACATAASATQLCADTIRLGKYDIGIAV
                     GMDKHPRGAFTDDPAKLALPQWYAENGQFVTTKFFGIKANRYIHDHGISQATLAKVAA
                     KNFRNGALNPNAFRRKPIPEEEILNSTVLNYPLTQYMFCAPDEGAAAVIMCRGDIAHR
                     FSSKPVYLRAAEIRTRRFGAYEVHATSAPIDEDVSPTVYASRAAFEVAGVSPDDVDVI
                     QLQDTDAGAEVIHMAEAGFCADGDQEKLLADGATEISGPMPVNTDGGLIANGEPIGAS
                     GLRQVHELVRQLRGEAGDRQVPGKPQVGFAQVYGAPGTAAATVLTL"
     gene            complement(1202..1615)
                     /locus_tag="VF874_10680"
     CDS             complement(1202..1615)
                     /locus_tag="VF874_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX9176516.1"
                     /translation="MQKALAPEISTWPEENPQLIGSQCGACGATAFPQQDHCPRCSAG
                     DMSELLLPRQGTLVAWTTQGFPPGPPYAGPTGKDFVPFGVGLVQLDDVIRVEGRLTEG
                     DPEKLQFGMDVELTMIPFTTDDEGNEVVTFAFRPL"
     gene            complement(1677..2525)
                     /locus_tag="VF874_10685"
     CDS             complement(1677..2525)
                     /locus_tag="VF874_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9176517.1"
                     /translation="MVLTRGAVSLDGRVAVVTGGGSGIGRGIAVAFAEFGARVAVWEK
                     DLDTAQAASAETGGLGCVTDVRDADQVDAAMSTTVETLGVPTILVNNAGGVFWSALLD
                     TSPNGWDALIRSNLTHVLLCTQRVARAMVDAGRGGSIINVTSIEGFRAAPGYAAYAAA
                     KAGVVNFTKTAALELAPRGIRVNGLAPDLTITEGILNMAPPGLDVAGPAAPDFEQRTS
                     LMIPMGRPGHVDEMAGAAVFLASDLSSYVTGQTIHVDGGTAAASGWYHDPGDGHYILG
                     PSGVRT"
     gene            complement(2525..3445)
                     /locus_tag="VF874_10690"
     CDS             complement(2525..3445)
                     /locus_tag="VF874_10690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9176518.1"
                     /translation="MRYAITHPIISHPYNPELVSGQGIAEVAAAAEAAGFHGFGFTDH
                     PAPTQRWLEAGGHDALDPFVAMGFAAARTTTLRLIPNIVVLPYRNPFVVAKAGATLDL
                     LSEGRFTLAVGAGYLKREFAALGVDFDERNELFDEALEVIRAIWTTDDVNFEGRHFTA
                     RGITAHPLPTSRPHPPIWIGGNTGRARQRVAEHGDGWCPFAAPAGLANTAGTAAMDTI
                     ERLRDGIDDLRRRFDAAGRDWSSVDISFNNFAGGSPGQPDFNADAYLTGLDKLAAMGV
                     TWVQGSLPGDSLAHALEAIASFGELVIAKA"
     gene            3551..4849
                     /locus_tag="VF874_10695"
     CDS             3551..4849
                     /locus_tag="VF874_10695"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9176519.1"
                     /translation="MTSPSLLEPDSGLPRGVQGAANPHFSGVVKLFTQLFPGRRFGGG
                     ALSVYIDGKPVVDVWTGWSDRKGEVPWSADTGAMVFSATKALASTVIHRLADKGLLDY
                     DAAVADYWPEFGANGKSDITIRDVLRHRSGLSHLKGVSKAELMDHLVMEERLAAAPVD
                     HLRGAPAYHALTYGWLLSGLARSVTGKDMRELIRTELARPLNTDGLHLGRPPADAPTT
                     VAQILAPQSTRPNPLFNFVAPRVAGLPFSGALGAMYFPGIKSLVQGDIPFLDGEVPAA
                     NGVVTGRGLAKMYAALANEGRIDGKRFLSRELARGLAGKPRVVPDLNMVVPMPFHLGY
                     HESPIPGLLKGFGHVGLGGTLGWADPQTGSAFAFVHNRLLTPMLFDMASFAVLARPLR
                     TAIAAARSSGAHTVPDFGAPYPKPARRRAAVTGARSRACP"
     gene            complement(4818..6242)
                     /locus_tag="VF874_10700"
     CDS             complement(4818..6242)
                     /locus_tag="VF874_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9176520.1"
                     /translation="MREYLKFYIDGQWVDPIEPKTLDVDNPTTEEVSGRISIGTAADV
                     DKAVKAARRAFASYSQTSREERLDLLQAIQAEYQKRAGDLAEAVTEEMGAPPSLAAGP
                     QVAMGMGHLASAIEVLKNFPFEEQHGSTLVVKEPIGVCGLITPWNWPINQIACKVFPA
                     LATGCTMVLKPSEVAPYSGYIFTEIIDAAGVPAGVYNMVNGDGPGVGAAISSHPDIDM
                     VSFTGSTRAGVDVAKNAAATVKRVTQELGGKSPNIVLDDEAFAQSVVSGVSVMMVNSG
                     QSCNAPSRMLVPKSRMDEAISLARDVAEKVVVGHPEDKSAIGPVASKAQFDKIQGLIQ
                     KGIDEGATVVTGGLGRPEGLDTGYYVKPTVFADVNNDMTIAREEIFGPVLCVLGYDDV
                     DQAVEIANDTDYGLAGYVSSGDLDKAREIARRIRAGNVAINHAFDLNAPFGGYKHSGN
                     GREWSEFGFHEYLEIKGTLGYAPQ"
     gene            complement(6255..7961)
                     /locus_tag="VF874_10705"
                     /pseudo
     CDS             complement(6255..7961)
                     /locus_tag="VF874_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979849.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
     gene            complement(7967..9010)
                     /locus_tag="VF874_10710"
     CDS             complement(7967..9010)
                     /locus_tag="VF874_10710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176521.1"
                     /translation="MSSSFEEFHDELRSVASDLLAKNTVVDWTALVEAGWVGLEVSDE
                     LGGAGATFREVAIVCEEMGRAASTSSYLGSAVLTVAALNTLQPSSFRDQLLSDIAAGT
                     VRAAVAVGSFTLDSNQRLSGRAEFVPDADGADRLLVVATDPDGIPVVVEATGAARPAT
                     SGVAAVMAQPVLDETRRQAIVEANDIAAEAVWRFEQGFQQLRDRAAVAIACDSLGLAE
                     AMLSATVSYAGVRHQFGRPIGSFQAVKHACADMAVQIAVARQLVGAAVDAVADDRPDA
                     TTAASMAKAYACGAAVDVVGKAMQLHGGIGYTWESGIHVYLKRAALNRSLFGSPAAHR
                     KHLAQRYLNTIQE"
     gene            complement(9010..10188)
                     /locus_tag="VF874_10715"
     CDS             complement(9010..10188)
                     /locus_tag="VF874_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176522.1"
                     /translation="MTAGLDEFRATVRTWCDEHIPADWRRSQTDISDEEFVAFQKWWF
                     AELRSAGFAVPHWPREWGGGMPVAEQVVLYQELAAHDAPRLVLAFVGIHHAAATLLAA
                     GTDEQRRRHLPAILDGEIWVQGFSEPEAGSDLASLRTSARKDGDTYVVNGQKLWASGG
                     MHADWCLLLARTDPNAPKRKGISYFLLDMKTPGVDVRPIRNAIGDSHFCEIFLNDVAV
                     PAANLVGAENAGWQVAQATLGAERGLTMLELAERLRHAGFRWLVETCATARMLDDPVV
                     QDRLAQFEIEIAGLRGLCRDLVDRTEAGTVGPADASVVKLFYSELLQRLTDFGTEIGG
                     LAAHTRLAKPMSSGWESGCWVLDFIGSWEWTIPGGSSEIQRTIIGERGLGLPREPSAV
                     "
     gene            complement(10185..10967)
                     /locus_tag="VF874_10720"
     CDS             complement(10185..10967)
                     /locus_tag="VF874_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9176523.1"
                     /translation="MKYDLPDELTVEADGPVRTVVINRADELNCVNENLHWALANVWR
                     QLAADKDAKVVVLTGAGRAFCAGGDLNWITTFLDDPVARDESIREGAQIIEEMLRFPL
                     PIIAAVNGPAVGLGCSVALLCDIMLISESAYLADPHVAVGLVAGDGGAAFWPLLGPIM
                     RTREYLYTGDRIPAATAVELGLASRAVAPDALTAQARTLADRLAAQPAEGLRGTKRIV
                     NMYLSQVLGGPLQAGFAAERATMQTQDHRYNLSAFQRKSTRT"
     gene            complement(10970..11938)
                     /locus_tag="VF874_10725"
     CDS             complement(10970..11938)
                     /locus_tag="VF874_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9176524.1"
                     /translation="MNRPLKVAVIGTGFGKHAAAPAYQSLDFEVEVVSPRDEAAVQRA
                     MAADVDLVSVHSPPFMHLQHVMGAIDHGHAVLCDKPFGRNAEEAAAMRDRARDGGIMH
                     FLNFEFRWKDSWAKIKQLADAGTIGAPTHLSWTFFGSGLRGRRYGWINDRELGGGWIG
                     AYGSHVIDFTRWLFHSEMAECGGVTRIEGLKHLDRDGQACNATAEDAYSAWFVMDNGC
                     TAAQDTGFATAAPTVPRATLMGSAGSIELIADTKLVVRRTGEEPETYEFPLPQARTPD
                     RALVAFFHQVADALRTGTQIAPSFDDGVAVAEAMDRLRANAIGATC"
     gene            complement(11944..12866)
                     /locus_tag="VF874_10730"
                     /pseudo
     CDS             complement(11944..12866)
                     /locus_tag="VF874_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            complement(12863..14023)
                     /locus_tag="VF874_10735"
     CDS             complement(12863..14023)
                     /locus_tag="VF874_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176525.1"
                     /translation="MGPEATRLRAELRDLVKTHMPENFLGAFTDDPADLEAAQRFCRL
                     LAEQGLLCMSWPEEFGGAGASIWEQTVVREEMWAHHEPRGAQYMGVNWVGPIIMRHGT
                     PEQQRRHLPPIARGEVIWCQGFSEPEAGSDLASLRTAARRDGDGWLVSGQKIWTSYAT
                     MAQWCFLLARTSKGEKKQKGLTIFLVPMDDPTIQVRPIRAMLGPHHLNEVFFDDLRVT
                     EADVLGEVDAGWSIVQDVMSFERVGIARYARCERLLQLAPAVLGDRWEELPEELRGRR
                     ARMLTHCRRARLLAYRVVSLQNSGQVKPGDAAAYRIAVTKLDQDSAEVLMEIAAAVPR
                     NNTAGAPRDERASRYFRAEVEDHWRYSQASTVSSGSIEMQRILLSRAMLAAS"
BASE COUNT         2238 a         4860 c         4608 g         2418 t
ORIGIN      
        1 ggtttcggcg aaaatcccga tctctccgca gtttcggcta gaaatcacag cgtcaacacg
       61 gttgcggcgg cggtgccagg agctccgtag acctgcgcga atcccacctg cggcttcccc
      121 ggcacctgac ggtcgccggc ctcgccgcgc agctggcgca ccaactcgtg cacctgccgt
      181 aacccggacg cgccgatcgg ctcgccgttg gcgatcagcc cgccgtcggt gttgaccggc
      241 atcggaccgc tgatctcggt tgcgccgtcg gccagcagct tctcctggtc cccgtcggcg
      301 cagaaccccg cctcggccat gtggatcacc tcggcgcccg cgtcggtgtc ctgcaactgg
      361 atcacgtcga cgtcatcggg cgacacgccg gccacctcga acgcggcgcg cgacgcatac
      421 accgtcggcg acacgtcctc gtcgatcgga gccgatgtcg cgtgcacctc gtaggcgccg
      481 aaccggcggg tgcgaatctc ggccgcccgc aggtacaccg gcttcgacga gaagcggtgc
      541 gctatgtcgc cgcggcacat gatcaccgcc gcggcgcctt cgtcgggcgc gcagaacatg
      601 tactgcgtga gcgggtagtt cagcaccgtc gagttgagga tctcttcttc tggaatcggc
      661 ttgcgccgga acgcgttcgg gttcagcgcg ccgttgcgga agttcttcgc ggccactttg
      721 gccagcgttg cttgcgagat gccgtggtcg tggatgtagc ggttggcctt gatcccgaag
      781 aacttggtgg tgacgaactg cccgttctcc gcataccact gcggcagcgc cagcttcgcg
      841 ggatcgtcgg taaacgcgcc gcgggggtgc ttgtccatgc ccaccgcgat cccgatgtcg
      901 tacttgccca atcggatcgt gtcggcgcac agttgcgtgg cgctggccgc ggtggcgcat
      961 gcgttgaaca cgtcggtgaa cgggatgccg gtgaggccca ccagccgggt caccgcgtcc
     1021 gggttggaca cctcatagct gcccccgaca ccgaactgga tgtccttcca ctcgaggccg
     1081 gcgtcgtcga gggcgccccg gatcgcctcg gcgcccatct gcatcgcggt cttgtcgaac
     1141 cggccgaacg ggtgcaggcc tacgccgatg atcgccacat cgttcatgac gcgatctcct
     1201 ttcagagcgg ccggaaggcg aacgtcacga cctcgttgcc ctcgtcgtcg gtggtgaacg
     1261 gaatcatggt cagctcgacg tccatgccga actggagctt ttccgggtcg ccctcggtga
     1321 gccggccctc cacacggatc acgtcgtcga gttggacgag gcccaccccg aacgggacga
     1381 aatcttttcc cgtcgggccg gcgtacggcg gccccggtgg gaagccctgc gtcgtccatg
     1441 ccacgagcgt gccctgccgg ggcagcagca actcggacat gtccccggca ctgcaccgcg
     1501 gacagtggtc ctgctggggg aacgcggtcg ccccgcaggc accgcactga ctgccaatga
     1561 gctgcgggtt ttcctcgggc caggtcgaga tctcgggtgc gagcgccttc tgcatcgagc
     1621 gatcctccgt gggtcaacta ccgaaatcgt ataatggcat tacactaaaa cagaaatcag
     1681 gttctcacac cgctgggtcc cagaatgtag tggccgtcgc cggggtcgtg gtaccagccc
     1741 gatgcggctg ccgtcccgcc gtcgacgtga atcgtctggc ccgtgacgta cgagctgaga
     1801 tcggacgcga ggaacacggc ggcgccggcc atttcgtcga cgtgccccgg gcggcccatc
     1861 ggaatcatca ggctggtccg ctgctcgaag tccggagccg cagggccggc gacatccagc
     1921 cccgggggcg ccatgttcaa aatcccctcg gtgatggtca ggtcgggcgc caggccgttg
     1981 acccggatcc cgcgcggagc gagttccagc gcggcggtct tggtgaagtt gacgacgcca
     2041 gccttggccg cggcatacgc ggcgtaaccg ggtgcggccc ggaacccttc gatcgacgtg
     2101 acgttgatga tgctgccgcc gcgaccggcg tcgaccatcg cccgggcgac tcgctgggtg
     2161 cacagcagga cgtgggtcaa attggaacgg atcagcgcgt cccaaccgtt cggagacgta
     2221 tcgagcagcg cggaccagaa cacgccaccc gcgttattca ccagaatcgt tggtacgccg
     2281 agggtttcga cggtcgtgga catcgccgcg tcgacctgat cggcgtcgcg aacgtcggtg
     2341 acgcacccga gcccgcccgt ctcggctgac gccgcctgcg ccgtgtcgag gtccttctcc
     2401 cacaccgcga cgcgggcacc gaactcggcg aacgctaccg cgatgccgcg cccgatgccg
     2461 gaccctccgc cggtgaccac cgccacccgt ccgtcgaggg acacggcgcc gcgcgtcaga
     2521 accatcaggc tttcgcgatc acgagctcac cgaaactggc gatcgcctcc agcgcgtggg
     2581 ccaagctgtc acccggcagc gacccctgca cccacgtcac gcccatcgcc gcgagcttgt
     2641 ccaggccggt caggtacgca tcggcgttga agtcgggctg gccagggctg ccgccggcga
     2701 agttgttgaa gctgatgtcg accgacgacc agtcccgccc ggcggcgtcg aaccggcgcc
     2761 gcaagtcgtc gatgccgtcg cgaaggcgct cgatcgtgtc catcgccgcc gtgcctgccg
     2821 tgttcgccag accggccggc gcagcgaacg ggcaccagcc gtcgccgtgt tcggcgaccc
     2881 gctggcgggc cctgccggtg ttgcccccga tccagatcgg tggatgcggg cggctcgtcg
     2941 gcagcgggtg cgcggtgatt ccgcgggcgg tgaaatgcct gccttcgaag ttgacgtcgt
     3001 cggtggtcca gatcgcccgg atcacctcca gcgcttcgtc gaacagctcg ttgcgctcgt
     3061 cgaaatccac acccagagcg gcgaattcgc gcttgaggta acccgcgccg accgcaagcg
     3121 tgaaccggcc ctcggagagc agatccagcg tggctcccgc tttcgcgacc acgaacgggt
     3181 tgcggtacgg cagcaccacg atgttcggga tcaaccgaag cgtcgtcgtc cgcgccgcgg
     3241 cgaaccccat tgcgacgaac gggtcgaggg cgtcgtggcc gcccgcctcg agccaacgct
     3301 gcgtcggcgc cgggtggtcg gtgaaaccga agccgtggaa tcccgccgcc tcggcggccg
     3361 ccgcgacctc ggcgatgccc tggcccgata cgagctccgg gttgtatggg tggctgatga
     3421 tcgggtgcgt gatcgcgtag cgcatctgtc ctctgtcgtc ggcgacggtt acgtcatttc
     3481 cctcagatgg taacgtcatt ctcatgtatc agtgccgata ctggctttgc gggaccgtct
     3541 catgagcacc atgacgtcgc cgtctcttct cgagcccgac agcggcctgc cccgcggcgt
     3601 gcagggggcg gccaatccgc atttctcggg cgtcgtcaag ttgttcaccc agctgtttcc
     3661 cggccgtcgg ttcggcggcg gggcgctgtc ggtgtacatc gacggcaaac ccgtggtgga
     3721 cgtctggacg ggatggtcgg atcgtaaggg cgaagtgccg tggtccgccg acaccggcgc
     3781 gatggtgttc tcggcgacca aggcgctcgc gtcgacggtc atccaccggc tggccgacaa
     3841 gggcctgctc gactacgacg ccgccgtcgc cgactactgg ccggagttcg gcgcgaacgg
     3901 caagtccgac atcacgatcc gcgacgtgct gcgtcatcga agcgggctgt cgcacctgaa
     3961 gggggtcagc aaggcggagt tgatggatca cctcgtgatg gaggaacggc tggccgccgc
     4021 gccggtcgat cacctgcggg gcgctcccgc gtatcacgcg ctcacctacg gctggctgct
     4081 gtccgggctg gcgcgttcgg tgaccggcaa ggacatgcgg gaactgatcc gtaccgagct
     4141 cgctcgcccg ctgaacaccg acggtctgca cctcggccgg ccgccggcgg atgcaccgac
     4201 cacggtggcg cagatcctcg caccgcagag caccaggccc aacccgctgt tcaacttcgt
     4261 cgccccgaga gtggccggtc taccgttctc gggggccctc ggtgcgatgt acttccccgg
     4321 catcaaatcc cttgtgcagg gcgatatccc gtttctggac ggcgaggtgc ctgccgccaa
     4381 cggtgtcgtc accggccgtg gcctcgccaa aatgtacgcc gcactggcca acgagggccg
     4441 aatcgacgga aagcggttcc tgtcgcgcga actggcccgc ggcctggcgg gcaagccccg
     4501 cgtggtgccc gacctgaaca tggtcgtgcc gatgccgttc catctgggct atcacgaatc
     4561 gccaatcccc gggctgctca aggggttcgg ccacgtcggc ctgggcggaa cactcggatg
     4621 ggccgatccg caaaccggga gcgcattcgc cttcgtccac aaccggctgc tgacgccgat
     4681 gctgttcgac atggcgtcgt tcgcggtgct ggcgcggccg ctgcgcacgg ccatcgccgc
     4741 cgcgcgctcg tccggcgcgc acaccgtgcc ggacttcggc gcgccctatc ccaagccggc
     4801 ccgccgacgg gcggccgtca ctggggcgcg tagccgagcg tgcccttgat ctccaggtac
     4861 tcgtggaagc cgaactcgct ccactcgcgg ccattgccgc tgtgcttgta gccgccgaac
     4921 ggcgcgttca aatcgaaggc gtggttgatc gcgacgttgc cggcacggat ccgacgagcg
     4981 atctcccgcg ccttgtcgag gtcgcctgag gagacatagc cggccagacc gtagtccgtg
     5041 tcgttggcga tctccacggc ctggtcgacg tcgtcgtaac cgaggacgca cagcaccggc
     5101 ccgaagatct cctcccgcgc gatcgtcatg tcgttgttca cgtcggcgaa cacggtgggc
     5161 ttgacgtagt agcccgtgtc cagaccctcg ggccgcccca ggccgccggt gaccaccgtc
     5221 gcgccctcgt cgataccctt ctggatcagg ccctggatct tgtcgaactg cgccttcgat
     5281 gccactggcc cgatcgcgct cttatcttcc gggtggccga caacgacctt ctcggcgacg
     5341 tcgcgcgcga gcgaaatcgc ttcgtccatc cgcgatttcg gcaccagcat gcgcgacggc
     5401 gcattacaac tctgaccgga gttcaccatc atcaccgaaa cgccgctgac cacgctctgc
     5461 gcgaaggctt cgtcgtcgag gacgatgttc gggctcttgc cgcccagttc ctgggtcacc
     5521 cgcttcaccg tcgccgcggc gttcttcgcg acgtcgacgc cggcgcgggt cgacccggtg
     5581 aaggacacca tatcgatgtc gggatggctc gaaatcgccg cgccgacccc cggaccgtcg
     5641 ccgttgacca tgttgtacac cccggccggc acccctgcgg catcgatgat ctcggtgaag
     5701 atgtagcccg agtacggtgc gacctcggat ggtttcagca ccatcgtgca gccggtcgcc
     5761 aaggcgggga acaccttgca cgcaatctgg ttgatcggcc agttccacgg agtgatcaga
     5821 ccgcacacgc cgattggctc cttgaccacc agtgtcgagc cgtgttgctc ctcgaacggg
     5881 aagttcttca gcacctcgat ggccgatgcg agatggccca tccccatggc gacctgcggc
     5941 ccggccgcca gcgacggcgg agcgcccatc tcctcggtca ccgcctcggc gaggtcgccg
     6001 gcccgcttct ggtactcggc ctggatcgct tggagcaggt ccagtcgttc ctcccggctg
     6061 gtctgcgaat agctggcgaa ggcgcgacgt gccgccttga cggccttgtc gacgtcagct
     6121 gcagtaccga tcgagatccg gccggacact tcttcggtgg tggggttgtc gacatccagg
     6181 gttttcggct cgatcgggtc gacccactgg ccgtcgatgt agaacttcag atattcgcgc
     6241 atggctgtct ccttttactg cgtgccttct gcataccacg tccgaaactc gtcgtacttc
     6301 ggcatctcct ccgaattggc cttcgggtcg atgcagacat ggacgacggc tgtcttcccg
     6361 ctggcgtacg cgcgctcgat cgccggaccc atgtcgtcct ctttctcgac gtactcgccg
     6421 tggcaaccga agccctcggc gatcttgtcg aacctcacgt ccttgctcca gagcaccccc
     6481 ggttgcggcg agggctgctc gaaggtgcgc ttttagacgc cgacttcgag gccccactgg
     6541 tggtcgacac ccacgacaca caccagcggc aggttcaagc gcgccgctgt ctccaattcg
     6601 gcgatgtgaa agaggaacgc cgagtcgctg gtcagcagca tgagcgggcg tttgccgcct
     6661 tcggcgacgg aagcgccgac agcgtagggc agcccggtgc cgaggtggcc gaagttctgg
     6721 ttccagatca cgtcgtgcgg cttggcctgc gagtaggtcc actggaagat cacggtggcg
     6781 ccgccgtcgc ggaccatgat gccgtcggcc gggaacgcct tggtcgcctc gacgacgaag
     6841 cgtgcgggat ggacgggagt tctgccggtc ggcgcgttct ccgccagttg cgccaattcc
     6901 tcggcgtcct tcttgatcag cacgtccaga tcgggcgacg gtgatcgcgg actgtctttc
     6961 agcgcttcga caagctgcgg aacgacaccg cgcaggtcgc cgacgagggg tacatcgacc
     7021 gggcggttga caccgatcgc gacgggatcc tgctccacgt agacccactt gcggttggcg
     7081 ttgttctctg cccagtggcg cgtcttcccg taatggaccg gctcgccgag ctcggtgcct
     7141 agtgcgacgc agagatccga cttgaccaca gcttcgacag ccgatggcga gaagccgtag
     7201 gcgaacgtgc gatcctcaag cccggcgatg aacgacgtgc cgccggaggt ctgaatcacc
     7261 gggcatgcca tcaggtcggc gagctctttg actgccgcgc cggtgcgcga cgtgtgcacg
     7321 ccgtgtccga caagcaggat cgggctcttg gcgtcgcgga tcagcttcgc agcctcagcg
     7381 atctcgcgtg cgcccgcgcc ctggtcgacg aggcgatacc ggttcggcgg caacggatcc
     7441 ggcacatcga gctcttcgag gatcacgtgc gaggggaact cgatgtagga cggacccggc
     7501 gtaccggaca tcgcgcggcg gatcgcctca cgcacgatct cgtcggtctg gtcggcgtac
     7561 tcgatggacg agctgtactt caccgacggg gcgaaaagcc gttcctgctg gacgaattgg
     7621 atccgtccgc ggcgcactcg ccgctcggtg atgcgggcac gctggccgcc gagaaagatc
     7681 accggcgagt tctccacgta cgcgcacatc atcgccccgg cgatgttggc gacacccggt
     7741 ccgagcgtac cgatgcacag gccgggcttc ccggtcatcc gcgaggccgc ctccgccatg
     7801 aacccggcgc tcaactcgtg gtgcggcgct accaccgacc aaccgcgcgc gtcggcctcg
     7861 gagaacatgt gcacgaaatt cgggtccgga atgccgaaga gcgtattgac gccctcggcc
     7921 tcgaacaggt cgagaatgcg tttgtaaacg gggacaccca tgttcgttac tcctgaatag
     7981 tgtttaggta ccgctgtgcg aggtgttttc ggtgtgccgc gggcgagccg aagagcgagc
     8041 gattcagcgc ggcgcgtttg aggtagacgt ggatgccgct ctcccacgtg tatccgatgc
     8101 cgccgtgcag ttgcatcgcc ttgccgacga cgtccactgc cgcgccgcag gcataggcct
     8161 tggccatcga cgccgcggtg gtggcgtccg gccgatcgtc cgcgacggca tccacggcgg
     8221 cgccgacgag ctggcgggcc accgcgatct gcacggccat gtccgcgcac gcgtgcttga
     8281 ccgcttgaaa cgatccgatg ggacggccga actggtggcg cacaccagca taggacaccg
     8341 tcgcggacag catcgcctcg gccagcccca ggctgtcgca ggctatggcc accgccgcgc
     8401 gatcacgcag ctgctgaaac ccttgctcga accgccagac cgcctcggcg gcaatgtcgt
     8461 tcgcttcaac aatggcctgc cgacgggttt catcgagcac cggctgcgcc atcacggctg
     8521 cgacgcccga tgtcgccggc cgagcggctc cggtggcctc gacgacgacc gggatgccgt
     8581 cgggatctgt cgcgaccacc agcagccggt cggcaccgtc ggcgtccggc acgaactccg
     8641 cgcgcccgga gaggcgctgg ttgctgtcga gtgtgaacga cccgactgcg acagcggccc
     8701 ggacggtgcc tgctgcgatg tcgctcagca gttggtctcg aaagctgctg ggctgcagtg
     8761 tattcagggc cgcgacggtc agcaccgcgc tgcccagata gctgcttgtg ctggcagccc
     8821 ggcccatctc ttcgcagacg atcgcgactt cacggaacgt cgcgcccgca ccgccgagtt
     8881 cgtcggacac ctctagccct acccatccgg cttcgacgag cgcggtccaa tcgacgacgg
     8941 tattcttggc gagcagatcg cttgccaccg agcgtaactc gtcgtggaac tcctcgaagc
     9001 tggaactcat cagacggcgc tcggctctcg aggtagcccc aaaccccgct caccgatgat
     9061 ggtgcgctgg atctcgctcg acccgcccgg gatcgtccac tcccaggacc cgatgaagtc
     9121 gagcacccag cagcccgact cccagccact cgacatgggt ttggccaggc gtgtgtgggc
     9181 ggctagtcca ccgatctcgg taccgaaatc ggtgagccgc tgcagcagct cgctgtagaa
     9241 gagcttcacc accgacgcgt cggccggtcc gacggtgccg gcctcggtac ggtccaccag
     9301 gtcccggcac aagccgcgca gacccgcgat ctcgatctcg aactgcgcca acctgtcctg
     9361 gaccacggga tcgtcgagca tccgcgccgt cgcgcacgtc tcgaccagcc accggaaccc
     9421 ggcatggcga aggcgctcgg cgagttccag catcgtcaat ccccgctcgg cgcccagagt
     9481 tgcctgtgcg acctgccatc ccgcgttctc cgcgccgacg aggttcgccg ccggaacagc
     9541 cacgtcgttg aggaagatct cgcagaaatg cgagtcgccg atcgcgttgc ggatgggccg
     9601 cacgtcgacg ccgggtgtct tcatgtcgag caggaagtac gaaatacctt tgcgcttggg
     9661 tgcattcggg tcggttcgcg ccagcagcag gcaccagtcg gcgtgcatgc ctccgctggc
     9721 ccacagcttc tgcccgttga cgacgtaggt gtcgccgtct ttacgtgccg acgtgcgcag
     9781 gctggccaag tcggatccgg cttcgggttc ggagaagccc tggacccaga tctcgccgtc
     9841 gaggatcgca ggtaagtgac gccgtcgctg ctcgtcggtg cccgcggcga gcaatgtggc
     9901 tgccgcgtgg tggattccga cgaacgcgag gacaagccgc ggcgcgtcgt gggcggcaag
     9961 ctcctgatac aggacgacct gctcggcaac cggcatcccg ccgccccatt cgcgcggcca
    10021 gtgcggtacc gcgaagcccg cgctgcgaag ctcggcgaac caccacttct ggaacgcgac
    10081 gaactcctcg tcgctgatat cggtctgcga tcgtcgccag tctgcgggga tgtgctcgtc
    10141 gcaccacgtt cgcaccgtgg cgcggaactc gtcgaggccg gccgtcatgt gcgtgtcgac
    10201 tttcgctgga acgcagagag gttgtagcga tgatcctgcg tctgcatcgt ggctctctcc
    10261 gccgcgaaac ccgcctgcag cgggccaccg agcacctggg acaggtacat gttcacgatc
    10321 cgcttggttc cccgaagccc ctcagctggt tgagcagcga ggcgatccgc cagcgtgcgc
    10381 gcctgcgcgg tcagcgcatc gggtgccacg gcgcggctgg ccaggccgag ttccactgct
    10441 gtcgcggcgg ggatgcggtc gccggtgtac aggtattcgc gcgtcctcat gatcgggccg
    10501 agcaacggcc agaacgcggc tccaccgtcg ccggccacca gcccgaccgc tacatgcggg
    10561 tcggcgaggt acgccgactc cgatatcagc atgatgtcgc ataacagcgc gacgctgcag
    10621 cccaatccga ccgcgggtcc gttgaccgcg gcgatgatcg gcagcgggaa gcgcagcatc
    10681 tcctcgatga tctgcgcgcc ctcgcggatg ctctcgtcgc gcgcgacggg atcgtcgagg
    10741 aaggtggtga tccagttcag gtcgccgccg gcgcaaaacg cccgcccggc gccggtgagt
    10801 accaccacct ttgcgtcctt gtcggcggcc agctgccgcc agacgttggc gagcgcccag
    10861 tgcaggttct cgttgacgca gttgagttcg tcggcgcggt tgatcacgac ggtgcgcacc
    10921 ggcccgtcgg cttcgacggt cagctcgtcg ggaagatcgt acttcaggtt cagcacgttg
    10981 ccccgattgc gttggcccgc aatcgatcca tcgcctccgc gacggcgaca ccgtcgtcga
    11041 acgacggcgc gatctgcgtg ccggtccgca gcgcgtcggc cacctggtgg aagaacgcga
    11101 ccagcgcccg gtccggcgtt cgcgcctgcg gcagcgggaa ctcgtaggtc tcgggctcct
    11161 cccccgtgcg ccgcacgacg agcttcgtgt cagcgatcag ctcaatggag ccggcgctgc
    11221 ccatgagcgt ggcgcgcggg accgtcgggg cggcggtcgc gaacccggtg tcctgcgcgg
    11281 ccgtgcagcc gttgtccatg acgaaccagg cggagtacgc gtcctccgcg gtggcgttgc
    11341 aagcttgtcc gtctcggtcc agatgcttga ggccctcgat ccgggtcact ccgccacact
    11401 cggccatttc gctgtggaac agccaacggg tgaagtcgat cacgtgcgat ccgtaggcgc
    11461 cgatccatcc gccgccgagc tcgcggtcgt tgatccagcc gtacctgcgg ccgcgaagcc
    11521 cgctgccgaa aaaggtccaa ctcagatgcg ttggggcgcc tatggtccca gcgtccgcca
    11581 gctgcttgat ctttgcccac gagtccttcc accgaaactc gaagttgagg aagtgcatga
    11641 ttccgccgtc gcgggcccga tcgcgcatgg cggccgcctc ctcggcgttg cgtccgaacg
    11701 gcttgtcaca gagcaccgcg tggccgtggt cgatcgcccc catgacgtgt tggaggtgca
    11761 tgaacggcgg cgagtggacg gagacgagat cgacgtcggc agccattgcg cgctgcacgg
    11821 ccgcttcgtc gcgtgggctg acgacctcga cttcgaagtc gaggctctga taagccggag
    11881 ccgccgcatg cttaccgaat ccggtaccga tgaccgcgac tttcaaaggc ctgttcatcg
    11941 gaatcaccgc ccaccggcgc tgtagaggcc cgtcagcccg cgccggccga gacggcgggt
    12001 caggtgatcg cgagttgcgg acaggccgaa cggaagtcga cgcagcggct gactgtagcg
    12061 cgacagccag gacagcgtcg tctcgtcgca gaagccgacc gcaccgtgca gttggtggca
    12121 gacccggaag acgacttcgg cggcctcgat cgcagccagc cgcagcgcca gcgcgtcgtc
    12181 gagcgcgtcg tcggatccga tgctccacag cgcgtacttg gcgaggatgt caacaccgct
    12241 gcgctcgacc tcggcgtcgg tcagctgaaa ctgaacacct tggaacgacg acaacgactg
    12301 accgaactgc ttgcgcagcc gcacgtgcgc gacggtgagt tcgattgccg atccagcatc
    12361 ccgagcaggg tccaacacgg cagcacaatc ccgagcgcga cgtcaccggc gccgccatgg
    12421 tcgatgtctg agagctccag ggcggcgaca aaggccgggc cagtgacggt ctggccgacc
    12481 acgctgcatc ggtggccatc caaagtgacg gcggcccacc gtttttggat gccgccgatc
    12541 gcagccacgg gggccgcggc atcgactacg atcagcccgt ctacgtcgag atcggtcggc
    12601 cgcgacagcc gctccgccac cgggtacggc aatgcccagt agcccgcgct gcggcacagc
    12661 gcggcagcgg cctccaggga atcggggtcg gtgcgagggt cgagctccca tgcgcccagt
    12721 ccgtcgagga ccggggcgac gagggcctcc cgggtgtcgg gcttggcctc ggcctgctgc
    12781 agcagctggt cgccgccggc tgattcgaac gcgcgaaggg cttcgcgccc atactctttg
    12841 gcttcgtcgc cgagatcgag gatcatgacg ccgccagcat ggcacgcgac agcaggatgc
    12901 gctgcatctc gatgctgccg gatgacaccg tggacgcttg cgagtaccgc cagtggtcct
    12961 ccacttcggc gcggaagtat cgggaggcgc gctcgtcgcg cggcgcaccc gctgtgttgt
    13021 tgcgcggcac agccgcggcg atttccatca gcacctcggc actgtcctgg tccagcttgg
    13081 tgaccgcgat ccgatatgcc gccgcatcac cgggtttcac ctgccccgag ttctgcagcg
    13141 acaccacacg gtaggcgagc aggcgggcgc gtcggcaatg ggtgagcatc cgcgcccgcc
    13201 gtccgcgcag ttcctcaggc aactcctccc agcggtcacc gagcacggca ggcgcgagtt
    13261 gcagcagtcg ttcgcagcgg gcgtagcgcg cgattccgac ccgctcgaag gacatcacgt
    13321 cctgcacgat cgaccagccg gcgtccacct cgccgagcac gtcggcctcc gtgacgcgca
    13381 ggtcgtcgaa gaacacctcg ttgagatgat gcggacccag catcgcccgg atcggccgga
    13441 cctggatcgt cggatcgtcc atcggcacaa ggaagatggt caggcccttt tgtttcttct
    13501 cccctttcga ggtccgggcg agcaagaagc accactgcgc catcgtcgcg taggacgtcc
    13561 agatcttctg gccgctgacg agccagccgt caccatcccg gcgcgccgcc gtccgcagtg
    13621 acgcgagatc cgagccggcc tcgggctcgg agaagccctg gcaccagatc acctcacccc
    13681 gcgcgatcgg cggcagatgt cggcgctgct gttccggtgt gccgtggcgc atgatgatcg
    13741 gcccgaccca gttgaccccc atgtactgtg cgccgcgcgg ctcgtggtgc gcccacatct
    13801 cctcgcgcac gacggtctgt tcccagatcg acgcgccggc accgccgaac tcctcgggcc
    13861 aggacatgca gagcaggccc tgctcggcga gcagtcggca gaaccgttga gccgcttcca
    13921 aatcggcggg gtcatcggtg aaggcgccga gaaagttttc gggcatatgc gttttcacca
    13981 aatcgcgcag ctctgcgcgc agccgcgtcg cctccgggcc catctcgaag tccacagtca
    14041 ctcctatccg gaacccagtg ccggttcggc gtccgcggcg gtcagcccga gttcgtcgag
    14101 cacctccgat gtgttctcac ccag
//
LOCUS       DASWBF010000297        12561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_758282,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000297 DASWBF010000000
VERSION     DASWBF010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12561
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758282"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /gene="rpsG"
                     /locus_tag="VF874_10740"
     CDS             <1..367
                     /gene="rpsG"
                     /locus_tag="VF874_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX9176526.1"
                     /translation="KSLAERIVYGALEQARDKTGTDPVVTLKRALDNVKPALEVRSRR
                     VGGATYQVPVEVRADRSTTLALRWLVGYSRQRREKTMVERLANEILDASNGLGASVKR
                     REDTHKMAEANRAFAHYRW"
     gene            439..2544
                     /gene="fusA"
                     /locus_tag="VF874_10745"
     CDS             439..2544
                     /gene="fusA"
                     /locus_tag="VF874_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9176527.1"
                     /translation="MAQKDVLTDLSKVRNIGIMAHIDAGKTTTTERILYYTGISYKIG
                     EVHDGAATMDWMEQEQERGITITSAATTTFWKGHQINLIDTPGHVDFTVEVERSLRVL
                     DGAVAVFDGKEGVEPQSEQVWRQADKYDVPRICFVNKMDKIGADFYFSVKTMEDRLGA
                     NVIPIQLPIGSESEFEGVVDLVEMNAKVWRGETKLGETYDTIDIPADLAEKADEYRTK
                     LLEAVAETDEELLEKYVGGEELTVEEIKGALRKLTINSEAYLVLCGSAFKNKGVQPML
                     DAVVDYLPSPLDVPAATGHVPGKEEAEVVRTPSTDEPFAALAFKVATHPFFGKLTYVR
                     VYSGKVDSGSQVINATKGKKERLGKLFQMHSNKENPVDSASAGHIYAVIGLKDTTTGD
                     TLADPNHQVVLESMTFPDPVIEVAIEPKTKSDQEKLSLSIQKLAEEDPTFKVHQDVET
                     GQTVIGGMGELHLDILVDRMRREFKVEANVGKPQVAYKETIRRVVDKVEFTHKKQTGG
                     SGQFAKVIISIEPFRGEDGATYEFENKVTGGRIPREYIPSVDSGAQDAMQYGVLAGYP
                     LVNLKVTLLDGAYHDVDSSEMAFKIAGSQALKKAAAAAQPVILEPIMAVEVTTPEDYM
                     GDVIGDLNSRRGHIQAMEERGGARVVKAHVPLSEMFGYVGDLRSKTQGRANYSMVFDN
                     YAEVPAQVSKEIIAKATGQ"
     gene            2709..3899
                     /gene="tuf"
                     /locus_tag="VF874_10750"
     CDS             2709..3899
                     /gene="tuf"
                     /locus_tag="VF874_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX9176528.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPDLN
                     ESRAFDQIDNAPEERQRGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMD
                     GAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADAVDDEELLELVEMEVRELL
                     ANQEFDEDAPVIRVSALKALEGDEKWVESIVELMAAVDESIPDPVRETDKPFLMPVED
                     VFTITGRGTVVTGRVERGVVNVNEEVEIVGIRPSTTKTTVTGVEMFRKLLDQGMAGDN
                     VGLLLRGIKREDVERGQVVVKPGTTTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFY
                     FRTTDVTGVVTLPEGTEMVMPGDNTNISVKLIQPVAMDEGLRFAIREGGRTVGAGRVT
                     KINK"
     gene            complement(4003..5394)
                     /locus_tag="VF874_10755"
     CDS             complement(4003..5394)
                     /locus_tag="VF874_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9176529.1"
                     /translation="MKSAQESERSRWTLAAVSTALFCVQLDYFAMNLALPRMAQDLGS
                     STTNLQWVISVYMLSLGAFMVPAGRIGDMFGRRGALLTGVALFGIASVLCAVALSAGM
                     LIAFRALQGLGAALIFPVSVSVLTNAFSAERSGRAIGMAYGIAGLGNAAGPLVGGLLT
                     ETVGWRSIFWLNVPFTVISLVIGALSIAESSDDTVPRRIDLAGLALIIAGVGLFTVTF
                     DRAASWGWTSVATSVAFVTSVVSLGAFVVVERRVRWPLVDLSLMRNAKFTILVIAGTV
                     ANIAYGVTIFLSTMYLQQVRGLNPLTAGLVFLGPSAGAAVGGALSGWLATRRPPVLVM
                     GTACVAAAISLAALSISAGGAVYLIALTATGSTLGLVYAFTTVATQAVVRPERAGEAA
                     GVTLTCLVTLVGVGVAVSGTVLEILQRSGSSTGAGIDVIIGALAAILLPAGALVVLVA
                     RAREGSPNSTPGL"
     gene            5507..6346
                     /locus_tag="VF874_10760"
     CDS             5507..6346
                     /locus_tag="VF874_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX9176530.1"
                     /translation="MFARYLKSQLMVLLCGGLVGPIFLVVYFALGPSARPYIAWMFFV
                     GLFITAADVLIALALANWGAKSEAKTAALEQSGVLALAQITGMGETGTRINDQPLITL
                     NLRIEGPGITPFEGTDRVIASVTRMGNFNQRKLVVLVDPATNKYQIDWERSALVNGLV
                     PAQFTVAEDNKTYDLSGQAGPLMEILQLLKANGVPLNRMVDVRSNPGLRQQIQAIVRR
                     AAAPQPPVAPAPVAAPSPAPAFTPPEPSISQRLQELETLRATGAVTEGEYQAKRTQII
                     SEI"
     gene            6369..6968
                     /locus_tag="VF874_10765"
                     /pseudo
     CDS             6369..6968
                     /locus_tag="VF874_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016693063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            7024..7869
                     /locus_tag="VF874_10770"
     CDS             7024..7869
                     /locus_tag="VF874_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEX9176531.1"
                     /translation="MAGSLEGRVAFVTGAGRGQGRAHAIRLANEGADIIAIDICAPVA
                     PSIAYPLATVEDLEETVRGVEAAGRKILARHVDVRDGAALNQVVADGVEQFARLDILV
                     ANAGVLSWGRLWELSEEQWNTVIDVNLSGTWRTIRAAVPAMIEAGNGGSIIIVSSSAG
                     LKATPGNGHYAASKHGLVALTNSLALEVGEFGIRVNSIHPYSIATPMIEREAMMELFK
                     SHPSYLHSFSPMPVHPTDHGGKRHRNEFMAPEEVSDVVAWLAGDGSATLSGVQIPVDR
                     GALKY"
     gene            complement(7866..8747)
                     /locus_tag="VF874_10775"
     CDS             complement(7866..8747)
                     /locus_tag="VF874_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9176532.1"
                     /translation="MTVVVGYLAGKGGLAPLHLAVEAARTLKTSLTVATIVPKPWTTP
                     SLAKIDAEYAKWAEQLAADSAREAQRYLGALARGLQVSYQNWAHRSVSGGLIEVLEPV
                     DADFLVLGSSANGQLGQVVVGSTADRLLHSSPVPVAISPRGYRGSRRGSLARITCAYP
                     GTPESVHVVERVVELAKRFDVPMRVITFAVRGRTMFPPEVGLHAEDTILAAWAAHARE
                     LLAQLKTDRVVGEDVVLQVVTGNDWGEALDAVAWDDGELLALGTSPRGGIARVFLGSR
                     GAKIVRHSPVPVLVLPG"
     gene            complement(8747..10255)
                     /locus_tag="VF874_10780"
     CDS             complement(8747..10255)
                     /locus_tag="VF874_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9176533.1"
                     /translation="MTSPSISLTDQMLRRRPVIGAPVAHGASDHLKRSIGTFQLTMFG
                     VGATVGTGIFFVLSQAVPEAGPGVIISFLIAGLAAGLAAICYAEMASAVPVSGSTYSY
                     AYTTLGEFVAMGVAACLLLEYGVSTAAVAVGWSQYLSKLLENIFHVGLPHAISAAPWD
                     KVEPGDPHAWINLPAVVLVAMCAVLLIRGASESAKVNAVMVVIKLGVLLMFAAVAFTA
                     FQADRFADFAPFGFGGISLAAGSIFFSYIGLDTVSTAGDEVKDPQHTMPRAIIAALLI
                     VTTVYVLVAVAALGAQPWEEFGDQKAGLAVILDNITGNIAGSTILAAGAVISIFSVTL
                     VVMYGQTRILFAMGRDGLLPSVFAKVSPRTMTPVTNTVIVAIVVAILAGFLPLDYLAD
                     MVSIGTLTAFIVVSIGVIILRVREPDLPRGFKVPLYPVTPILSIAVCAYILFSLRWYT
                     WIAFSAWVAVALIFYLVWSRHHSALNDGGDGVIPTAAAGVEDVEVVTPPKEQ"
     gene            complement(10284..11519)
                     /gene="rocD"
                     /locus_tag="VF874_10785"
     CDS             complement(10284..11519)
                     /gene="rocD"
                     /locus_tag="VF874_10785"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEX9176534.1"
                     /translation="MTIVDAAVADETQAAIALEKRHAARNYSPLPVVAASAEGAWIFD
                     VEGRRYLDCLAAYSAVNFGHRHPAIVASAHAQLDSVTLLSRAFHSDRLGPFCKALTDL
                     CGKDMVLPMNSGAEAVESGLKVARKWGTDVKRVPAGRANIIVADNNFHGRTISIISFS
                     SDPAARDGFGPFTPGFRSVPFGDAAALARAIDDDTVAVLLEPIQGEAGIIVPPDDYLP
                     TVRALCSEYNVLMIADEVQSGLARTGRTFACEHWGVVPDMYLLGKALGGGVVPLSAVV
                     ANQDVLGVLHPGEHGSTFGGNPLATAVGSTVVALLETGEFQRRSKELGAHLHRRLRGL
                     LGDGVLAVRGLGLWAGVDIDPACGTGKEISLRLAERGVLVKDTHGSTLRFAPPLVITA
                     QEIDWAVDQFAVVLARVRS"
     gene            complement(11516..12379)
                     /locus_tag="VF874_10790"
     CDS             complement(11516..12379)
                     /locus_tag="VF874_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HEX9176535.1"
                     /translation="MTEYLFAADDACRDAPSRLGARTPRTRRYVMTPPEFFAVEYAIN
                     PWMDVAAPVDRHIAARQWESLYETYLRLGHLVELVRPEAGLPDMVYAANGGFVAGDTA
                     VLARFKFAERAPESVAYAAWMQERGYRAAPTRHVNEGQGDLLLVGEMVLAGYGFRTDI
                     RAHAEIAQLLRMPVISLELVDPRFYHLDTALAVLDDTTIAYYPPAFSEAAQGQLRALF
                     PDAIVVGSADAYVLGLNAVSDGLHVVYPSGAMGFADQLRDAGFEPVGVDLSELLKGGG
                     SIKCCTLEVHP"
BASE COUNT         2444 a         4055 c         4116 g         1946 t
ORIGIN      
        1 gaaatcgctg gccgaacgca ttgtttatgg tgcgctcgag caggctcgcg acaagaccgg
       61 caccgacccg gtggtcaccc tcaagcgcgc tctcgacaac gtcaaacccg ccttggaggt
      121 tcgtagccgc cgcgtgggtg gcgcgaccta ccaggttccc gtcgaggtgc gcgccgaccg
      181 ctccaccacg ctggccctgc gctggctggt gggctactcg cggcagcgtc gggagaagac
      241 gatggtcgaa cggctggcca acgagatcct cgacgcgagc aacgggctgg gtgcctcggt
      301 gaaaagacgc gaggacaccc acaagatggc cgaggcgaac cgggccttcg cgcactaccg
      361 ctggtgacaa gcgctggcgg gagcagctgc cgggcgtaac gacaacagca accaagcaac
      421 cgaaagagtg agacttccgt ggcacagaag gacgtgctga ccgacctgag caaggtccgc
      481 aacatcggca tcatggcgca catcgatgcc ggcaagacga cgacaacaga gcgcatcctc
      541 tactacaccg gtatcagcta caagatcggt gaggtgcatg acggcgccgc cacgatggac
      601 tggatggagc aggagcagga gcggggtatc accatcacct cggctgctac cacgacgttc
      661 tggaaaggcc accagatcaa cctgatcgac acccccgggc acgtcgactt caccgtcgag
      721 gtggagcgca gcctgcgggt gctcgacggt gccgtcgcgg tgttcgacgg taaggaaggt
      781 gtcgagccgc agtccgagca ggtgtggcgg caggccgaca aatacgacgt cccgcgcatc
      841 tgcttcgtca acaagatgga caagatcggc gcggacttct acttctccgt caagacgatg
      901 gaagaccgcc tcggcgcaaa cgttatcccg atccagctgc cgatcggctc tgagagcgag
      961 ttcgagggcg tcgtcgacct ggtcgagatg aacgccaaag tgtggcgcgg cgagacgaag
     1021 ctgggcgaga cgtacgacac gatcgacatc ccggccgacc tcgccgagaa ggccgacgag
     1081 taccgcacca agctgctcga ggccgtcgcg gagaccgacg aggaactgct ggagaagtac
     1141 gtcggcggtg aagagctcac ggtcgaggag atcaagggtg cgcttcgcaa gctgacgatc
     1201 aactccgagg catacctggt gctgtgcggc agcgcgttca agaacaaggg cgtgcagccc
     1261 atgctggacg ccgtagtcga ctaccttcca tcgccgctgg acgtgccggc cgcaacgggc
     1321 catgttcctg ggaaggaaga agcagaggtc gtccgtacac cgtcgaccga cgagccgttc
     1381 gccgcgttgg cattcaaggt cgccacgcac ccgttcttcg gcaagctgac ttatgtccgg
     1441 gtgtattcgg gcaaggtcga ttccggttca caggtcatca acgccacaaa gggcaagaag
     1501 gagcggctgg gcaagctgtt ccagatgcac tccaataagg agaacccggt ggactcggcg
     1561 tcggccggtc acatctacgc ggtgatcggg ctgaaggaca ccaccaccgg tgacacgctc
     1621 gccgacccga accatcaggt ggttctcgag tcgatgacgt tccccgaccc ggtgatcgag
     1681 gtagcgatcg agcccaagac caagagcgat caggaaaagc tgagtctctc gattcagaag
     1741 ctcgccgagg aagatccgac cttcaaggtg caccaagacg tcgagacggg ccagaccgtg
     1801 atcggcggga tgggtgagtt gcacctggac atcctggtgg accgcatgcg ccgcgagttc
     1861 aaggtcgagg ccaacgtcgg caagccgcaa gtggcataca aggagaccat ccgccgcgtg
     1921 gtggacaaag tcgagttcac ccacaagaag cagaccggcg gctcaggcca gttcgcgaag
     1981 gtcatcatct cgatcgagcc cttcaggggc gaagacggtg cgacttacga gttcgagaac
     2041 aaggtcaccg gcggccggat cccgcgcgag tacatcccgt cggtggactc cggtgcgcag
     2101 gacgcgatgc agtacggcgt gctcgccggc tatccgctgg tgaatctgaa ggtcacgctc
     2161 ctcgacgggg cgtaccacga cgtcgactcg tccgaaatgg cgttcaagat cgccggctcg
     2221 caggcgctga agaaagcggc cgccgcggcg cagccggtga tcctggagcc gatcatggcc
     2281 gtcgaggtca ccacgcctga ggactacatg ggcgacgtga tcggcgacct gaactcccgc
     2341 cgtggtcaca tccaggccat ggaggagcgc ggcggtgccc gcgtcgtcaa ggcgcatgtg
     2401 ccgctgtcgg agatgttcgg ctacgtcggc gaccttcggt cgaagaccca gggccgggcg
     2461 aactactcca tggtgttcga caactacgcc gaagtcccgg cgcaggtgtc gaaggagatc
     2521 atcgcgaagg cgacgggtca gtgaggatcg aggccgtagg ccgaggcccg gagctgaccc
     2581 ggaggcgagc aatcgattca gcgaaggcga cgggtcagta gaccccgtcg gtttcgccga
     2641 ccacacaact gaaagaatca accctgcttc actagcaagc accaacagaa gtccaggagg
     2701 acaacacagt ggcgaaggcg aagttcgagc ggacgaagcc gcacgtcaac atcgggacca
     2761 ttggtcacgt tgaccacggc aagacgacgc tgaccgcagc aatcaccaag gttctgcacg
     2821 acaagtttcc cgatttgaac gagtcgcgcg cattcgacca gatcgacaat gcgcctgagg
     2881 agcgtcagcg cggcatcacg atcaacatct cccacgtgga gtaccagacc gagaagcgtc
     2941 actacgcgca cgtcgacgcc ccgggccacg ccgactacat caagaacatg atcaccggcg
     3001 cagcccagat ggacggcgcg atcctcgtgg tcgccgccac tgacggcccg atgccgcaga
     3061 cccgcgagca cgtgctgctg gcgcgtcagg tcggcgtgcc ctacatcctg gtggcgctga
     3121 acaaggccga cgcggtcgac gacgaggagc tgctcgagct cgtcgagatg gaggtccgcg
     3181 agttgctggc caaccaggag ttcgacgagg acgccccggt catccgggtc tcggcgctga
     3241 aggcgctcga gggcgacgag aagtgggtcg agtcgatcgt ggaactgatg gcggccgtgg
     3301 acgagtcgat ccccgacccg gtccgcgaga ccgacaagcc gttcctgatg cccgtcgagg
     3361 acgtcttcac gatcaccggc cgcggcaccg tggtcaccgg tcgtgtcgag cgcggcgtgg
     3421 tcaacgtgaa cgaggaagtc gagatcgtcg gcatccggcc gtcgacgacc aagaccacgg
     3481 tcaccggtgt ggagatgttc cgcaaactgc tggaccaggg catggccggc gacaacgtcg
     3541 ggctgctgct gcgcggcatc aagcgtgagg acgtcgagcg cggccaggtc gtggtaaagc
     3601 ccggcaccac cacgccgcac accgagttcg agggccaggt ctacatcctg tccaaggacg
     3661 agggtggccg gcacacgccg ttcttcaata actaccgtcc gcagttctac ttccgcacca
     3721 ccgacgtgac cggtgtggtg acgctgccgg agggtacgga gatggtgatg cccggtgaca
     3781 acaccaacat ctcggtgaag ctgatccagc ccgtcgccat ggacgagggt ctgcgcttcg
     3841 cgatccgtga gggcggccgc accgtcggcg ccggccgggt cacgaagatc aacaagtagt
     3901 tacgacgcgc acgcggcgct catccgaaag ggtgggcgcc gcgtgcgttt tcgggagccc
     3961 tcccacgtcg agatctacgt cagggtcgtg gttcctcgaa aatcacagcc ctggcgtcga
     4021 attcggtgag ccttcgcgtg cccgtgcgac gagcacgacg agcgcacctg cgggcagcag
     4081 gatggccgcg agcgcgccga ttatgacgtc gatgccggct ccggtcgacg aaccggatcg
     4141 ctgcaggatt tctagcactg tgccggacac cgcgacgccg acaccgacca acgtgaccaa
     4201 gcacgtcaac gtcaccccgg cggcttcccc ggcgcgctcc ggtcgcacga ccgcttgcgt
     4261 cgcgaccgtg gtgaacgcat acaccagtcc cagtgtggag ccggtcgcgg tgagcgcgat
     4321 caggtacacc gccccgcccg cggagatcga cagcgccgca agggatatcg ccgccgcgac
     4381 gcaagccgtc cccatcacca gcaccggtgg ccgtcgggtt gccaaccaac ccgacagcgc
     4441 cccgccaaca gcggcgcccg ccgacgggcc gaggaacacc aggccagccg tcaacgggtt
     4501 caagccgcgc acctgctgca ggtacatggt ggacaggaag atcgtgacgc cgtatgcgat
     4561 gttggcgacc gtgccggcga tcaccaagat cgtgaacttc gcgttgcgca tcagcgacag
     4621 gtcgacaagc ggccagcgga cgcgccgttc gacgacgacg aacgcgccga gcgacaccac
     4681 cgacgtgacg aacgcgacgc tggtcgcaac ggatgtccac ccccacgacg ccgcgcggtc
     4741 gaaggtcacc gtgaacaacc cgacgccagc gatgatcagc gccagcccgg ccaggtcgat
     4801 gcgtcgaggc acggtgtcgt ccgaggactc ggcaatgctg agcgcgccga tcaccaagga
     4861 gatgacggtg aacgggacgt tcagccagaa gatcgagcgc cagcccaccg tttcggtgag
     4921 cagaccaccc acgagtggtc ccgccgcatt acccaaaccc gctataccgt aagccattcc
     4981 gatcgcgcgg cccgaccgct cggcggagaa ggcgttggtg agtacgctga ccgacaccgg
     5041 gaagatcagc gcggcgccaa gcccctgcaa ggcgcggaac gcgatcagca tcccggccga
     5101 cagtgcgacc gcacacagca cggaggcgat gccgaacagt gccacgccgg tgaggagagc
     5161 tcctcggcgg ccgaacatgt cgccgatccg gcccgctgga accatgaacg cgcccaacga
     5221 gagcatgtag acgctgataa cccactgcag gttcgtcgtg ctgctgccca gatcttgtgc
     5281 catccggggc aacgcgaggt tcatcgcgaa gtagtcgagt tgaacacaga acagtgccgt
     5341 cgacaccgcc gccaacgtcc agcgcgagcg ctccgactcc tgcgctgact tcaccggccg
     5401 accatacgac gagctgccgc ctcagcaagt gtgcaatcgc agcgaacgtc gatgttcggc
     5461 gctaatctga cacgcgacgg gggctggcga ggcgaggagc atgctgatgt tcgcgcgata
     5521 cctgaagtcc cagttgatgg tgctgctgtg cggcggactg gtcgggccga tcttcttggt
     5581 cgtgtatttc gcactcggcc cgtcggcccg gccctacatc gcttggatgt tcttcgtcgg
     5641 cctgttcatc accgcggccg acgtgctgat cgcgctggcg ctggccaact ggggcgcgaa
     5701 gtccgaggcg aagaccgcgg cgctcgagca gagtggtgtg ctggcgctgg cccagatcac
     5761 aggtatgggt gaaaccggca cgcgtatcaa cgaccagccg ttgatcaccc tcaacctgcg
     5821 catcgaaggt cccggcatca cgccgttcga aggcacagat cgagtcatcg cgtccgtgac
     5881 tcgaatgggc aacttcaacc agcgtaagct cgtcgtgctc gtcgacccgg ccaccaacaa
     5941 gtatcagatc gactgggagc gaagcgcttt ggtgaacggc ctggtgcccg cgcaattcac
     6001 cgtcgccgag gacaacaaga cctacgacct gtccgggcag gccggcccac tgatggagat
     6061 cctgcaactc ttgaaggcca acggtgttcc gttgaaccgc atggtcgacg tgcgctcaaa
     6121 tcccggtctt cgccagcaga tccaggcgat cgtgcgccgc gccgcggccc cgcagccccc
     6181 ggtggcgcct gctcctgtcg cagcaccgtc gccggcgccc gccttcaccc cgcccgaacc
     6241 gtcgatctcg cagcggctgc aggaattgga gacattgcgc gcaacgggcg cggtgaccga
     6301 gggcgagtac caggccaagc gaacgcagat catctccgag atctaagtct caggggcggt
     6361 cgccaccgat cctcgcgcgt aacccgcgta gctacaccgg agtcgagtcc gacgacagcg
     6421 ccgtcgagtt gagccgaagg gccgtgggag acgacgatcg ggtgatccgt ggcgaggcgg
     6481 cccgcactgg tctgcccgat gcgagtgtcg acgctgtgct cggtgaggcg atgctgacga
     6541 tgcagacaga cgctcacaag gccgacatcg tggcggaggc gttccgggtg ttacgcccgg
     6601 cgggccgata cgcgattcac gaactcgcgt tgagaccgga tggcgtcgac gactcagtca
     6661 agacggagat ccgcaccgcg ctcgcgcgtt cgatcaaggt caacgcgcga ccgttgaccg
     6721 cgcccgagtg gacgaacttg ctcaccactg ccggattcga tgttcagaca gtgtctttcg
     6781 caccgatggc gctgttggag ccgcgccgtg tggtagccga cgagggcctg gcacccaccc
     6841 tgcggtttgt ggccaatgtt gtgggcgacg gcgacgcgtg gactcgtgtg cgaggaatgc
     6901 gcagcacctt ccgcaagcac agggagaacc tcgccgcgat ctcggtgatc gccgtcaagc
     6961 ccgattagcg aagtgcagct aacccactag aacacgtttc agtttcgctg ctagcctgag
     7021 cgcatggctg gatcactcga ggggcgcgtg gcgttcgtca ccggcgctgg gcggggccaa
     7081 ggccgtgcac acgcgataag actggcgaac gagggcgccg acatcatcgc gatcgacatc
     7141 tgcgcgccgg tggcgccgtc gatcgcctat ccgctagcga ccgtggaaga cctcgaagaa
     7201 accgtccgcg gggtggaagc cgcgggccga aagatcctgg cgcgtcatgt cgatgtccgc
     7261 gacggtgcgg cgctgaatca ggtcgtggcc gacggtgtcg agcagttcgc gcgcctggac
     7321 atcttggtcg cgaacgcggg cgtgctgagt tggggccggc tctgggaact gtccgaagag
     7381 caatggaaca ccgtgatcga tgtgaacctg tcgggcacct ggcggaccat ccgcgcggcc
     7441 gtccccgcga tgatcgaggc gggcaacggc ggctcgatca tcatcgtcag ctcgtcggcc
     7501 gggctgaagg ccacgccggg caacggtcac tacgcggcgt ctaagcatgg gctggtggcg
     7561 ctgacgaact cgctggccct ggaggtcggc gagttcggta tccgggtcaa ctccatccac
     7621 ccgtactcga tcgccacgcc gatgatcgag cgcgaggcga tgatggagct gttcaagagc
     7681 cacccgagct atctgcatag tttttcgccg atgccggtgc atcccaccga ccacggaggc
     7741 aagcggcacc gcaacgaatt catggcgccc gaagaggttt ccgacgtggt ggcgtggctg
     7801 gccggagatg ggtccgcgac gctgtcgggt gtccagatac ccgtcgaccg cggcgcgctg
     7861 aagtactaac ccggcaggac gagcaccggc actgggctgt ggcggacgat tttcgctccg
     7921 cgagacccca agaacacccg cgcaattccg ccccgcggtg acgtgcccag cgccagcagt
     7981 tcgccgtcgt cccacgccac agcgtcgagc gcctcgcccc agtcgtttcc ggtaactact
     8041 tgcagcacaa cgtcttcgcc gacaacgcgg tcggttttta actgcgccaa caactctcgg
     8101 gcatgtgcgg cccacgcggc caggatcgtg tcctcggcgt gcaacccgac ctcgggcgga
     8161 aacatcgtgc ggccgcgtac ggcgaacgtg atgacgcgca tcggcacgtc gaatctcttc
     8221 gccagctcga cgactcgctc gaccacgtga acggactcgg gcgtgccggg atacgcgcac
     8281 gtgatgcgcg cgagcgaacc cctgcgggag ccgcggtacc ctcgcgggct gatcgccacc
     8341 ggcacgggcg aggagtgcag caaccggtcg gcggtcgacc cgacgaccac ctggccgagt
     8401 tgcccgttgg ccgacgagcc gagcaccaag aaatccgcgt ccacaggttc aaggacttcg
     8461 atcaggccgc ccgacaccga ccggtgtgcc cagttctggt aggacacctg caatccccgt
     8521 gccagtgcgc cgagatagcg ctgcgcctca cgtgcggaat cggcggccaa ttgctcagcc
     8581 cacttggcat attcggcgtc gatcttggcc agcgacggcg tcgtccatgg cttcgggacg
     8641 atcgtcgcca cggtcagcga cgtcttcagg gtgcgggccg cttccacagc gaggtgcaac
     8701 ggtgcgagcc cgcccttgcc cgccagatag ccgacgacga cggtcatcac tgttccttcg
     8761 gcggtgtgac gacctcgacg tcttcgacgc cggcggcggc cgttgggatc acaccgtcgc
     8821 cgccgtcgtt gagcgcgctg tggtggcggc tccacaccag gtagaagatc agcgcgactg
     8881 ccacccacgc actgaacgcg atccaggtgt accagcgcag gctgaaaagg atgtaggcgc
     8941 agaccgcgat cgacaggatc ggcgtgaccg ggtagagcgg aaccttgaac ccgcggggca
     9001 gatccggctc gcgcaccctg aggatgatga cgccgatcga caccacgatg aacgcggtga
     9061 gcgttccgat cgacaccatg tcggccaggt agtccagcgg caagaacccg gcgaggatcg
     9121 ccaccacgat cgcgacgatg acggtgttcg tgacgggcgt catcgtgcga gggctgacct
     9181 tggcgaacac cgatggcaac aatccatcgc ggcccatcgc aaacagaatg cgggtctggc
     9241 cgtacatcac caccagcgtc accgagaaga tcgaaatcac ggctcccgcg gccagaatcg
     9301 tgctgcccgc gatgtttccg gtgatgttgt cgaggatcac cgccaggccg gctttttggt
     9361 ccccgaattc ttcccacggc tgagcgccga gcgcggctac cgcgacgagg acgtacacgg
     9421 ttgtcacgat cagcagcgcg gcgatgatcg cgcgtggcat cgtgtgctgc gggtccttga
     9481 cttcgtcgcc ggccgtcgat accgtgtcaa ggccgatgta ggagaagaag attgagcctg
     9541 cggccagact gatcccgccg aagccgaacg gggcgaagtc ggcgaaccgg tcggcttgga
     9601 acgcggtgaa cgcgacggcc gcgaacataa gcagcacgcc aagcttgatc accaccatca
     9661 ccgcgttgac tttggccgac tcgctggcgc cccgaatcaa cagaaccgca cacatcgcca
     9721 ccaagacgac ggcgggcagg ttgatccaag cgtgtgggtc tccgggttcc accttgtccc
     9781 acggtgcggc cgaaatcgca tgcggcagac ctacatggaa gatgttctcg aggagcttcg
     9841 acaggtactg gctccatccc acggcgaccg cggcggtcga gacgccgtac tccagcagaa
     9901 ggcaggccgc gactcccatc gcgacgaatt cgcccaacgt ggtgtaggcg taggaatagg
     9961 tggatcccga caccggcacc gccgaggcca tctcggcgta gcagatcgcc gcgaggccgg
    10021 ccgcaaggcc cgcgatgaga aacgagatga tcacgcccgg accggcttcg ggtacagcct
    10081 gcgacagcac gaagaagatg ccggtgccca ccgtcgcacc gacaccgaac atcgtgagct
    10141 ggaacgtccc gatgctgcgc ttgaggtgat cggatgcccc atgcgcgact ggggctccga
    10201 tgaccggccg ccgtctcagc atctgatctg tcaggcttat cgacgggctg gtcatgggag
    10261 cctcctcgtt cgcgactagc cgattatgag cgcacccgag ccaaaacgac agcgaactga
    10321 tcaaccgccc aatcgatctc ctgcgcggtg atcaccagcg gcggcgcgaa ccgcagcgtc
    10381 gagccgtgcg tgtccttgac gagcacgccc cgctcggcga ggcgcagact gatctctttg
    10441 ccggtgccac aggccgggtc gatgtcgacg cccgcccaca gacccaggcc gcgcaccgcc
    10501 agcacgccgt cgccgagcaa cccgcgcaac cggcggtgca ggtgagcacc caattccttt
    10561 gagcggcgct gaaactcgcc cgtctccagc aacgcgacca cggtagaccc gactgccgtg
    10621 gccagcggat tgccgccgaa cgtcgaaccg tgttcgccgg ggtgcaggac gccgaggacg
    10681 tcctggttgg cgaccacggc cgacagcggc accaccccgc cgcccagggc cttgccgagc
    10741 aggtacatgt cgggcactac gccccaatgc tcgcaagcga aggtacgccc ggtgcgcgcg
    10801 agtccggact ggacctcgtc ggcgatcatc agcacgttgt actcactgca cagtgcccgc
    10861 accgtcggca aatagtcgtc gggcggcacg atgatgccgg cctcgccttg aatcggctcg
    10921 agcagtaccg cgacggtgtc gtcgtcgatc gctctggcaa gcgcggcggc gtcgccgaac
    10981 ggaaccgagc ggaagccggg tgtgaacggg ccgaagccgt cccgggcggc cggatccgac
    11041 gagaagctga tgatgctgat ggtccggccg tgaaagttgt tgtccgccac gatgatattc
    11101 gcacggcctg ctgggactct tttgacgtcg gtgccccatt tccgcgcgac cttgaggccg
    11161 ctttcgaccg cttcggcacc gctgttcatc ggcagcacca tgtccttgcc gcacaagtcg
    11221 gtcagggctt tgcagaacgg gccgaggcgg tcggaatgga acgcacggct gagcagcgtc
    11281 acgctgtcga gctgggcatg ggccgacgcg acgatggcgg ggtggcggtg tccgaagttc
    11341 accgccgaat acgcggccag gcagtcgaga tagcgacggc cctcgacgtc gaagatccac
    11401 gcgccctctg cgctggccgc gaccactggc agtggcgagt agttgcgggc cgcgtgacgc
    11461 ttttccaaag cgatcgccgc ttgcgtctcg tcggcgactg ccgcatcgac aatcgtcatg
    11521 ggtgtacctc caatgtgcag cacttgatcg atccgccgcc cttgagcagt tcggacaagt
    11581 cgacgcccac cggttcgaaa ccggcatctc gcagctgatc tgcgaaaccc attgcaccgg
    11641 atgggtatac gacgtgcagt ccgtcggaga cggcgttgag tccgagcacg tatgcgtcgg
    11701 cactgccgac gacgatcgcg tcggggaaga gcgctcgcag ttggccttgc gccgcctcgc
    11761 tgaaggcggg tgggtagtac gcgatggtcg tgtcgtcgag caccgcgagg gccgtatcga
    11821 ggtggtagaa acgcggatcg accaactcga gtgaaatcac cggcatgcgt aacaactgcg
    11881 cgatctcggc gtgggcgcgg atgtcggtgc gaaagccgta gccggccaac accatttcac
    11941 cgacgagcag taggtcaccc tgtccctcgt tgacgtgccg agttggtgct gcgcggtatc
    12001 cccgctcctg catccaggcc gcgtaggcga cggactccgg ggcgcgttcg gcgaatttga
    12061 atcgcgccag cacggcggtg tcgccggcga cgaagccgcc gttggccgcg tacaccatgt
    12121 cgggcagccc ggcttcgggt cggaccagct cgacgaggtg gccgaggcgc agataggtct
    12181 cgtagagcga ctcccattgc cgagccgcga tgtgccggtc gaccggtgcg gccacgtcca
    12241 tccacggatt gatcgcgtac tcgacggcga agaactccgg cggcgtcatc acgtagcggc
    12301 gggtacgcgg cgtgcgggca ccgagtcgcg acggtgcgtc acgacaggcg tcatccgcgg
    12361 cgaaaaggta ctccgtcatc gaaaccacga tatttcggac cgttcgcgca atcaatcacc
    12421 atatgttgct tctgttgcga tactctgttg cgcgaatgcc tggaattatt cgatctgttg
    12481 cgtaaacact ggagggcgct acgtggaccg cctggatgac atcgacgagc gcatcttggc
    12541 cgagctggcc gagcacgcga g
//
LOCUS       DASWBF010000298         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_93578, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000298 DASWBF010000000
VERSION     DASWBF010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93578"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..1464
                     /locus_tag="VF874_10795"
     CDS             394..1464
                     /locus_tag="VF874_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013139345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HEX9176536.1"
                     /translation="MLIRLPAMQFRGGTSKGVYLLRDDLTPYADSLDAVLSALMGGAP
                     RQIDGLGGAATTTSKVAIIGRSSEDGIDVDYLFAQVDPQTGKVDWIPTCGNILAGVGP
                     FAIERGLVSVAGDTTAVRIRLLNTGARVVATVQTPNGAVEYSGDQAIAGVSGTAAPVQ
                     LTFTDFAGGTTGTLFPTGQRVDHIDGVEVSCVDAGVCAILMRATDLGMIGAESPAEIN
                     ADTALLDRIERVRLKAGELMGMGDVGGKVVPKVMLVSGSEVADVRSRYLVPTSCHPAH
                     AVSGAINLASSLSIPGTVAYGLGDHGSAPGGIGIEHPGGVIELAVTLDGDAPVGASIT
                     STARKLSDGVVFFEFDASAKER"
     gene            1470..2585
                     /locus_tag="VF874_10800"
     CDS             1470..2585
                     /locus_tag="VF874_10800"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX9176537.1"
                     /translation="MITRFGRWVSAAAIIAVLGVAFSEMRLPAGWLIAGMLGAATVAL
                     VSRRELNPPAKAMSAAQSVIGVVAVEPLTRLTGSDLVHFAGCAAFSVSITLGLSVVFG
                     IVLARFAKDLGLATANLSLIAGGASTISCMARELGADERYVALSQYLRLTIVVLTMPL
                     VLSQLGTADPTPRLVLAQDHWSWVGLLVLVVVIYAGARLARLARMPAPFLLGPLVVGA
                     VVAIADPSLARLMDPTAVVTGVAYVVIGWQAGGGFAVDVLRRFIKLIPLTLMFIALTI
                     GSCFAVAVAVSEWTGVSLADAYLATTPGGIYGVLAIANEVHSGPIVVTLQVLRLVTMV
                     LAAGMIPRIIGALQRRQAPATEPMVVESRSLSTAAVS"
     gene            complement(2678..>3221)
                     /locus_tag="VF874_10805"
     CDS             complement(2678..>3221)
                     /locus_tag="VF874_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9176538.1"
                     /translation="EAVDLALVDVYLPDGSGIDFVRELHCDSMMLTAATEAATIRAAI
                     AAGALSYLVKPFAPAELAARLAGYARYRRILAAPNLGGGEVDAALDALRPKIAQQQSS
                     ALPSPTKQLVLQALRASDKPMSSAEVSAAIGVSRATAQRYLATLATTAEVTIGLRYGT
                     TGRPEQEFLAAATTTNRRPS"
BASE COUNT          494 a          999 c         1129 g          599 t
ORIGIN      
        1 gtcggtagtc ggccgccgac agcaggtgcc gacggggccg aaagtgcggc gagatgccgc
       61 tgaacgctcg gctactgttt cgtgcctgag cccggcaact gacgtccagc cgccaagcac
      121 gcgagcaatc gccgccggcg ccatcctgct catccgaagc agggacttcg tccccgcaca
      181 accagagcac gcagcagcgg aggtatccgc taccgacact gccccttaga ttcgtgtagc
      241 ccgcccgccg ccggtccggg gtggaacacc gcatgcacac aatgcgcaaa aggtgaaaat
      301 cgcttgttgt gcgggttgcg cgcacaagcg cgaaaccctg gccgcgaaac cgtaacttcg
      361 gagcagaagc aagtccgaag aacggagttt gaaatgctaa ttcggttgcc cgccatgcaa
      421 tttcgcggag gaacgtctaa gggagtctat ctcctgcgcg acgacctgac gccctatgcc
      481 gactctctcg acgcggtgct gtcggcgttg atgggtggag cgccgcgcca gatcgacggg
      541 cttggcggcg ccgcaacgac gacgagcaag gtggcgatca tcggtcgctc gtcggaggac
      601 ggcatcgacg tcgactatct gttcgcccag gtcgacccgc aaaccggcaa ggtcgactgg
      661 atcccgactt gtggcaacat cctggccggg gttgggccgt ttgcgatcga gcgcggtctg
      721 gtctccgtcg cgggtgatac gaccgccgtg cggattcgcc tgttgaacac gggggcccgg
      781 gtcgtggcga cggtgcagac gccgaacggt gcggtcgaat acagcggtga tcaggcgatc
      841 gccggggtga gcggtaccgc cgcaccggtg cagctgacat tcaccgactt cgccggcggc
      901 accaccggaa cgctgttccc gaccgggcag cgcgtcgacc acatcgacgg tgtcgaagtc
      961 agctgcgtcg acgcgggcgt gtgcgccatt ctgatgcgcg ccaccgacct cggaatgatt
     1021 ggcgcagaaa gccctgccga gatcaatgcc gacactgccc tgctggaccg gatcgagcgg
     1081 gttcggctga aggcaggcga gctgatgggc atgggtgacg tcggcggcaa ggtggtcccg
     1141 aaggtgatgc tggtgtccgg ttccgaggtc gccgacgtcc ggtcgcgcta tctcgtgccg
     1201 acgtcatgcc acccggctca cgccgtgtcc ggcgcgatca acctggcatc cagtctgtcg
     1261 ataccgggaa ccgtcgccta cggtctcggc gaccacggca gcgctcctgg cggtatcggg
     1321 atcgagcatc ccggtggcgt gatcgagctg gccgtgacct tggacggcga cgcccccgtc
     1381 ggggcaagca tcacctcgac cgctcgcaag ctctccgacg gagtggtctt cttcgagttc
     1441 gacgccagtg cgaaggagcg gtgattccca tgatcactcg atttggacgt tgggtgtcgg
     1501 ccgcggcgat catcgcggtg ctcggcgtgg ccttctcgga gatgaggctg cctgccgggt
     1561 ggttgatcgc cggcatgctc ggtgcggcga ccgtcgcatt ggtatcacga cgggagctca
     1621 atccgccggc caaggcgatg tcggccgccc agagcgtgat cggtgtggtg gcggtcgaac
     1681 ctttgacgag gttgaccggt tcggatctgg tgcacttcgc gggatgtgcc gccttctcgg
     1741 tgagcatcac gctcgggttg agtgtcgtgt tcggcattgt gctcgcccgc ttcgccaagg
     1801 acctgggcct ggcgacggcc aatctgtccc tgatcgccgg tggtgcgagc accatttcct
     1861 gcatggctcg tgagctcggg gccgacgagc ggtacgtggc gctgtcgcag tacctgcggc
     1921 tgaccatcgt cgtgctgacg atgccactgg tgctgagtca actgggtacg gctgatccga
     1981 cgccgcggtt ggtgctggcg caggatcact ggagctgggt gggcctgctg gtgttggtcg
     2041 tcgtgatcta cgcgggtgcc aggctggccc ggctggcccg gatgccggca ccgtttctgc
     2101 tggggccgct ggtggtgggc gcagtggtcg cgattgccga tccctccctg gccaggttga
     2161 tggatccgac cgccgtggtg acaggcgtcg cctatgtggt gatcggctgg caggccggcg
     2221 gcggcttcgc cgtcgacgtc ttgcgcaggt tcatcaagct gatcccgctc acgctgatgt
     2281 tcatcgccct gacgatcgga agctgtttcg ccgtggccgt cgccgtcagc gaatggactg
     2341 gggtgtcgct ggccgatgca tacctggcga ccacgccggg gggcatatac ggtgtcttgg
     2401 ccatcgccaa cgaggtgcac tcgggaccga tcgtggtcac cttgcaggtg ctgcggctgg
     2461 tgacgatggt gctggccgcc ggaatgatcc cgaggatcat cggcgcactg cagcggcgcc
     2521 aggccccggc gaccgagccg atggtcgtgg agtccaggtc gttgtccact gccgccgtgt
     2581 cctgacgcct ccagaggtgt ttcgccgccc tgtcgcctgc gcacgagacg gtgcgcgggc
     2641 gatagggcgt ttggcgaacg gtcctgcggc ggagcgttca gctcgggcgg cggttcgtgg
     2701 tcgtggccgc tgccaggaat tcctgttcgg gtcgtccggt ggtgccatac cggagcccga
     2761 tcgtcacttc agctgtggtg gccaacgtgg cgagatagcg ttgggccgtc gccctcgaga
     2821 cgccgatcgc cgcggatacc tccgccgacg acatcggttt gtccgatgcc cgcaaggctt
     2881 gcagcactaa ttgtttggtc ggggagggca gcgcactgga ttgctgctgg gcgatcttgg
     2941 gacgcagggc atctaatgcc gcgtccacct cgccgccacc cagattcggg gcggcgagga
     3001 ttctgcggta acgggcatag ccggccagcc gggccgccag ctcagcgggc gcaaacggtt
     3061 tgaccagata gctcaacgcg cccgcggcta tggcggcgcg gatcgtggct gcttcggtcg
     3121 ctgccgtcaa catcatgctg tcgcagtgca attcgcgtac gaaatcgatg ccggatccgt
     3181 ccggaagata tacgtcgacc agtgcgaggt cgaccgcctc g
//
LOCUS       DASWBF010000299         9931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1277299,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000299 DASWBF010000000
VERSION     DASWBF010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277299"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VF874_10810"
     CDS             <1..1531
                     /locus_tag="VF874_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9176539.1"
                     /translation="NLEIALLARQANPNVRVVARLVNTVLRDEVAVGNGPGAILDVAD
                     LAASSVVEACLSRTTHAITIAGVEVVVSGTEAPRDATLRELFGDLAPVAVIRGESSPS
                     PGEVVACPGRDLTVQAGDWTAMLGTVDELAAQGIRAEPHTARQLYRRTRMVRMLDGLR
                     MIREDVNPVFYRLLGVTLALLVGSTVILRVAYQYPGMTWVDALYFSTETIATVGYGDF
                     NFIDQPTWLRLWGVAMMFAGVMTVAILVAFIADLLLSRRFAQSAGRRQVRHLRRHVIV
                     VGLGSFGIRVVSDLKAAGYEVAAIERNDDNRYLSAAAALDVPVIFGDATLRQMLEAAR
                     VSEARAVGVLTQDDMVNVEIGIVLKEILGPRWKRRGNRPGVPVVLRVYDRALGAAVSH
                     RFGFANVRSTVELATPWFIGAAMGLQVLGTFSVGQRSFMVGGVRVEPGSELDGLRMLE
                     MSTRTRVIAIGRDGGEFTLHPRRDTRLNAGDTAYLVGPYRELLATLRKGQFGISAAKT
                     G"
     gene            complement(1509..2270)
                     /locus_tag="VF874_10815"
     CDS             complement(1509..2270)
                     /locus_tag="VF874_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9176540.1"
                     /translation="MPTPPHGAPIWIDLSSSDVDRAQEFYGTVFGWTFEPSGPDYGGY
                     VNAFKDGKRVAGLMHNQPEWNAPDAWTIYFHTGDIQSTAAAIAPAGGSTCVEPMEVKE
                     KGWMAMGTDPSGAAFGLWQPTGHRGFEVLGEPGAPVWHQLTTRDFAKAVDFYRALFGW
                     QTRVESDTDEFRYTTAIFDGNDVLGIMDGSTFQPDGIPSHWTTFFGADDVDKTLQVLT
                     DHGGAVLRPAEDTPYGRLAAAADPSGAVFNLSSLR"
     gene            complement(2283..3212)
                     /locus_tag="VF874_10820"
     CDS             complement(2283..3212)
                     /locus_tag="VF874_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9176541.1"
                     /translation="MYEYGIDFAAVDAIDIHTHVEVDSHGHTATDGELSAATRQYFKL
                     GPESGSTVDTLAEHYRQRNIAAVVFTIDAESASGHVPNSVEDMVEGAARNNDILIPFG
                     TVDPWKGQRAVRRVHSLVHEYGVKGFKFHPSVQAFDPNDRRFYPIYEAIAEAGVPALF
                     HTGQTGLGAGLPGGYRIKLRHSDPILLDDVAADLPEMTIVMAHPAVPWVDSQIAIATH
                     KANVYIDLSGWSPKYFPPQLVHAANRQLKTKVLFGTDFPFIQVDRWRRDFETLDMDPD
                     VLPLIFKQNAMRVLGLPDQRDEFSGRHRSIPVS"
     gene            complement(3358..3537)
                     /locus_tag="VF874_10825"
     CDS             complement(3358..3537)
                     /locus_tag="VF874_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEX9176542.1"
                     /translation="MSFLDKAKDLLSKNADKVDTAIDKAADIVDSKTQGKYKDKVDKV
                     QEAAKKAVDKGNPQT"
     gene            complement(3586..5973)
                     /locus_tag="VF874_10830"
     CDS             complement(3586..5973)
                     /locus_tag="VF874_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX9176543.1"
                     /translation="MTDPAAPALATGLTDAEVAQRVAEGKTNDVPTRAARTVSEIVRA
                     NVFTRINAILGVLFVIVLATGSLINGLFGLLIIANSGIGIIQEIRAKQTLDKLAIVGQ
                     AKPLVRREGGTRELLPSEVVLDDVIELGPGDQIVVDGTVVEDVNLEVDESLLTGEADS
                     IAKDIGDPAMSGSFVVAGSGAYRATRVGHEAYAAKLAEEASKFTLVKSELRSGINTIL
                     RFITYLLIPAGLLIIYTQLFTTGAGWRESVLRMVGALVPMVPEGLVLLTSIAFAVGVV
                     RLGRRQCLVQELPAIEGLARVDVVCADKTGTLTENGMRVADTKPVSEADVGSVLASLA
                     ADDSRPNASMQAIAEAYDEPPGWTATAIAPFKSATKWSGVSYRDHGNWVIGAPDVLLN
                     PSSPVAEQAEEIGAQGLRVLLLASSDVPVDHPKAPGLITPAALVILEQRIRPDAHETL
                     DYFASQDVSIKVISGDNAVSVGAVASTLGLEGDTLDARRLPDDLDELANIMDRYATFG
                     RVRPDQKRAMVHALQSREHTVAMTGDGVNDVLALKDADIGVAMGAGSSASRAVAQIVL
                     LDNKFATLPHVVAEGRRVIGNIERVSNLFLTKTVYSVLLAVLVGIAGVGSKIFHTDPL
                     LFPFQPIHVTITAWFTIGIPAFILSLAPNNERAKPHFVRRVMTAALPSGLIVGTATFV
                     CYLLAYQGHDASDVQQTQASTAALITLLMSGVWVLAVVARPYTWWRVLLVVFAGLAYV
                     VIFSIPLAREKFMLDPSNVALTSMAMTIGLLAVAAIEASWWVQGRVIGDRRRLWR"
     gene            complement(5992..7512)
                     /locus_tag="VF874_10835"
     CDS             complement(5992..7512)
                     /locus_tag="VF874_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9176544.1"
                     /translation="MLLVACGSPQRSASPSSDVPPNEVSGIDIPAGRVDDAIGKLDGL
                     VDDLMKSSGIPGMAVAVVHDGKVAYVKGFGVSDVRTGAKVDADTVFQLASLSKPMGAT
                     VVAHEVTANAIGWDTPVSAKLPWFELADPYVTRTATIADLYSHRSGLPDHAGDILEDL
                     GYDRRQVLERLRYLPLAPFRITYEYTNFGLTAGAEAVAAAAGKPWDVLSDEVLYRPLG
                     MTSTSSRFADFQARANRAVGHVKTPDGYQPLYTRDPDAQSPAGGVSSSINDMARWLTL
                     VLGNGTYNGVRIASPEALLPTVTPQVISAPAANPNARASFYGHGFNVSVTSSGRTQFS
                     HSGAFGLGAATAFAALPSANVGLIALTNAAPIGVPETLGAQFMDLVQYGGIREDWPAL
                     YRQVFAEMDKPEGSLVGKQTPADPAPARPLNAYVGEYANPYWGPAKVAEVNGALELSI
                     GPRGDKFPLSHWDGDTFTFTVQSENAPPGTISKATFNGNTLTLEYFDTDKLGTFTR"
     gene            complement(7605..8720)
                     /locus_tag="VF874_10840"
     CDS             complement(7605..8720)
                     /locus_tag="VF874_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9176545.1"
                     /translation="MLRGALRVGIGTATLLGGGWLLRALRGAPAALGASPLAIREVAD
                     GSPNYRDGRFVNVDPASMIEVNREEQRILLREMLGGRAGRPSGEIPVVKPAGLGMPPA
                     PLAVTWFGHSTSLLEIDGYRVLTDPVWGDRCSPSHTVGPERLHPPPIPLEALPAVDAV
                     VISHDHYDHLDLDTVLALARSQRAPFVVPLGVGTHLRTWGIPQQRIIELDWDQSASVD
                     DLTLVCTPARHFSGRFVSRNTTLWASWVITGPQHRAYFGGDTGYTRSFAEIGSQHGPF
                     DLTLMPVGAYNRLWPDIHMNPEEAVRAHLDVSGPGKGLLVPIHWGTFRLAPHPWAEPV
                     DRLVDAAGSAGVGVAVPKPGHRIDLSEPSTLDAWWRL"
     gene            complement(8727..9458)
                     /locus_tag="VF874_10845"
     CDS             complement(8727..9458)
                     /locus_tag="VF874_10845"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9176546.1"
                     /translation="MIGVTRVDHVTTIEMQRPDRRNALNSELVEGLRKAIVQAVADDI
                     RAVVLTGQGSVFCAGADLSGDAFAEDYPDRLIALNKAIDEAPMPVIGAINGPAIGAGL
                     QLAMICDLRVVSPDAFFQFPTAKYGLALDNWSIRRLTSLAGHGRARSMLLTAEKLTAD
                     VALQTGMANRIGSLADAQKWAEEIAGLAPLALRHAKRVLNDDGAYEDQSPVHKELFER
                     AWASQDVIEAQVARMQKRQPNFKGA"
     gene            9500..>9931
                     /locus_tag="VF874_10850"
     CDS             9500..>9931
                     /locus_tag="VF874_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9176547.1"
                     /translation="MSRIPATVLRDAVLDAGSFVSWDTAPLDVAADDDYARDLAQARA
                     STGLDESVITGEGTVHGRRVAVVACEFDFLAGSIGVAAAERITAAVQRATRDRLPLLA
                     SPSSGGTRMQEGTVAFLQMVKIAAAVQQHKQVHLPYLVYLRH"
BASE COUNT         1572 a         3346 c         3346 g         1667 t
ORIGIN      
        1 caacctcgaa atcgccctgc tggcgcgtca ggccaatccg aacgtgcgcg tggtggcccg
       61 cctcgtcaac accgtcttgc gcgacgaggt tgcggtgggg aacggccccg gagcgatcct
      121 cgacgtagcc gaccttgcgg catcgtccgt cgtcgaagcc tgcctgtcgc gcaccaccca
      181 cgcgatcacg atcgccggcg tggaggtcgt cgtctccggc acagaggcgc cccgggatgc
      241 gacgctgcgc gaactgttcg gcgatctcgc gccggtcgcg gtgatcagag gcgagtcgtc
      301 gccgtccccc ggcgaggtgg ttgcctgccc aggacgggac ctgacggtgc aggccgggga
      361 ttggaccgcg atgttgggca ccgtcgacga gctcgccgcc cagggcatca gagccgagcc
      421 gcacacggcc cgtcagctgt atcggcgcac gcggatggtc cggatgctcg acgggctgcg
      481 catgatccgt gaggacgtca acccggtgtt ctaccgcctg ctgggagtta cgctggccct
      541 gctcgtcggc tcgacggtga tcctgcgggt cgcctaccaa taccccggaa tgacctgggt
      601 ggatgcgttg tacttctcca ccgagaccat cgccaccgtc ggctacggcg acttcaactt
      661 catcgaccag ccgacctggc tgaggttgtg gggcgtcgcg atgatgttcg cgggcgtcat
      721 gaccgtcgcg attctggtgg cattcatcgc cgacctgctg ctgtcgcggc ggttcgcgca
      781 gtcggcgggc cgccgccagg tacgccatct gcgccgccac gtgatcgtgg tagggctggg
      841 ctcgttcggc atccgcgtgg tcagcgatct caaggcggcc ggctacgagg tcgcggcgat
      901 cgagcgcaac gacgacaacc ggtacctgtc cgcggccgcc gcgctcgatg tcccggtcat
      961 cttcggcgac gccacactgc gccagatgct ggaggcggcg cgcgtttcgg aagcgcgcgc
     1021 cgtgggggtg ctcacccaag acgacatggt gaacgtcgaa atcggcatcg tgttgaagga
     1081 aatcctgggc ccgcgatgga agcgccgagg caaccggccc ggcgtgccgg tggtgctgcg
     1141 ggtctacgac cgcgcgctcg gcgccgcggt gtcccacagg ttcggcttcg cgaacgtccg
     1201 gtcgacagtc gagctggcga cgccgtggtt catcggcgcg gcgatgggac tgcaggtgct
     1261 cggcacgttc tcggtcggtc agcgctcgtt catggtgggc ggggtgcggg tggagcccgg
     1321 cagcgaactc gacggacttc gcatgctcga gatgtccacc cgcacccgcg tgatcgcgat
     1381 cggccgggac ggcggcgagt tcacgctgca cccgcgccgc gacacccggc tgaacgccgg
     1441 cgacaccgcc tacctggtcg gcccgtaccg ggaactgctg gccaccctgc gcaaggggca
     1501 gttcgggatc agcgcagcga agacaggttg aacacggcgc cgctgggatc ggcggcggcg
     1561 gccagccgcc cgtagggggt gtcctctgcg ggacgcagca cagctccgcc gtggtcggtg
     1621 agcacctgca acgtcttgtc gacgtcgtcc gcgccgaaga acgtcgtcca atgcgacggg
     1681 atgccatccg gctggaacgt actgccgtcc attatcccga gcacgtcgtt gccatcgaaa
     1741 attgctgtgg tgtaacggaa ttcgtcggtg tcggactcga cgcgggtttg ccagccgaac
     1801 agcgcgcggt agaagtcgac ggccttggcg aagtcgcggg tggtcagctg atgccagacc
     1861 ggcgcaccgg gctcgccgag cacctcgaag ccgcgatgcc cggtcggctg ccacagcccg
     1921 aacgccgcgc ccgacgggtc ggtgcccatc gccatccagc ccttctcctt gacctccatc
     1981 ggctcgacgc atgttgaccc gccggccggc gcgatggcag ccgcggtcga ctggatgtcg
     2041 ccggtgtgga agtagatggt ccaggcgtcg ggggcgttcc actcgggctg gttgtgcatc
     2101 agcccggcga cgcgcttgcc gtccttgaac gcgttgacgt agccgccgta gtcggggccg
     2161 gacggctcga aggtccagcc gaagacggtg ccgtagaact cctgggcgcg gtcgacgtcc
     2221 gatgacgaca ggtcgatcca gatgggcgcg ccgtggggtg gggtgggcat gtgagtctcc
     2281 tttcagctca ccggtatcga ccgatgccgc cccgaaaact catcgcgctg atcaggaagg
     2341 cccagcaccc gcatcgcgtt ctgcttgaag atcagcggca gcacgtcggg gtccatgtcg
     2401 agcgtctcga agtcgcggcg ccagcggtcc acctggatga acgggaagtc ggtgccgaac
     2461 agcaccttgg tcttcagctg acggttggcc gcatgcacca gctgcggcgg gaagtacttc
     2521 ggcgaccagc cggacaggtc gatgtatacg ttcgccttgt gggtggcgat ggcgatctgc
     2581 gagtcgaccc acggcaccgc ggggtgagcc atcacgatcg tcatctcagg caagtcggcg
     2641 gcgacgtcgt cgagcagaat gggatccgag tggcgcagct tgatcctgta gccgccgggc
     2701 aggcccgcgc cgaggcccgt ctgcccggtg tggaacagcg caggcacccc ggcctctgcg
     2761 atcgcctcgt agatcggata gaaccgccga tcgttcggat cgaacgcctg cacgctcggg
     2821 tggaacttga accccttcac gccgtattcg tggaccaggc tgtgcacgcg gcgcactgcc
     2881 cgctggccct tccacggatc gacggtgccg aacgggatca gtatgtcgtt gttgcgcgcg
     2941 gcgccttcga ccatgtcctc gacggagttg ggcacgtggc ccgaggccga ttccgcgtcg
     3001 atcgtgaaca ccaccgccgc gatgttgcgc tgacggtagt gctcggcaag cgtgtcgacg
     3061 gtcgagccgc tctccgggcc gagcttgaag tactgccgtg tcgcggcgct cagctcgccg
     3121 tcggtggccg tgtggccgtg gctgtcgacc tcgacatggg tgtggatgtc gatcgcgtcg
     3181 acggccgcaa aatcaatacc gtactcgtac atgaacttct cctgcattga ctctgcgccc
     3241 agggcgttgc cttctcgaac ttctgcgccc tcaccgcaga gtcaacgcgg cgggccgaac
     3301 aaggcggggc cgcccaggtg cgtcacaccc ggtggagacg gaaagcttgg ccattcgtca
     3361 ggtctgtgga tttcccttgt cgacggcctt cttcgcggcc tcctgcacct tgtcgacctt
     3421 gtccttgtac ttgccctggg tcttcgagtc gacgatgtcg gcggccttat cgatcgcggt
     3481 gtccactttg tcggcattct tggacagaag gtctttcgcc ttatccagaa aactcatcgg
     3541 atcaaccctt tcatcggtgt tctggtcatt ctcgtcgagc acgcgtcatc tccacaaccg
     3601 gcgccggtcg ccgatgaccc tgccctgcac ccaccatgac gcctcgatcg ctgccaccgc
     3661 cagcagcccg atcgtcatcg ccatactggt cagcgcaacg ttggacgggt cgagcatgaa
     3721 cttctcccgg gccagcggaa tcgaaaagat cacgacgtac gcaaggccgg cgaacaccac
     3781 cagcagcacc cgccaccacg tgtacgggcg cgcgaccacg gccagcaccc acacgccgga
     3841 catcagcagc gtgatcagcg ccgccgtcga cgcctgcgtc tgctgcacgt cgctggcgtc
     3901 gtggccctga tacgccagca ggtagcagac gaacgtcgcg gtgccgacga taagacccga
     3961 cggcaacgcc gccgtcatga cccggcgcac gaagtgcggc ttggcccgct cgttgttcgg
     4021 cgccagcgac aggatgaacg cgggaatgcc gatcgtgaac caagcggtga tcgtcacatg
     4081 gatgggctgg aacggaaaca gcagcggatc ggtgtgaaaa atcttcgatc ccacaccggc
     4141 gatgccgacc agcaccgcca gcagcaccga atacaccgtc ttggtcagga acaggttgga
     4201 cacccgctcg atgttgccga tcaccctgcg accctcggcc acgacgtgcg gcaacgtcgc
     4261 aaacttgttg tccagcaaca caatctgcgc gacggcccgc gacgccgagc tgcccgcgcc
     4321 catcgcgacg ccgatatcgg cgtccttcag cgccagcacg tcgttgacgc cgtcgccggt
     4381 catcgccacc gtgtgctcgc gagattgcaa cgcatgcacc atcgcccgct tctggtccgg
     4441 gcggacccga ccgaaggtgg cgtagcggtc catgatgttg gcgagttcgt cgagatcgtc
     4501 gggcaaccgg cgggcgtcga gcgtgtcgcc ttcgagcccg agcgtcgacg cgaccgcacc
     4561 caccgacacc gcgttgtcac cggagatcac cttgatcgaa acatcctgcg acgcaaagta
     4621 atccagcgtt tcgtgcgcgt cgggccgaat gcgctgctcg aggatcacca acgctgcggg
     4681 ggtgatcagg ccgggtgcct tcggatggtc gacgggcacg tcactggagg ccagcaacag
     4741 cacgcggagc ccttgcgcgc caatctcttc ggcttgctcg gcgacgggcg acgacgggtt
     4801 cagcagcacg tcgggtgcgc cgatcaccca gttaccgtgg tcgcggtagg acacgccgct
     4861 ccacttggtg gcggacttga acggcgcaat cgcggtggcc gtccagccgg gcggctcgtc
     4921 gtaggcctcg gcgatggcct gcatgctggc gttgggccgg ctgtcgtcgg cggcgagcga
     4981 cgccagcacg ctgcccacgt ccgcttcgct cactggcttg gtgtcggcca cccgcatccc
     5041 gttctcggtc agcgttccgg tcttgtcggc gcagaccacg tcgacgcggg ccaggccctc
     5101 gatcgcgggc agctcctgca ccaggcactg ccgtcggccc agccggacca cgccgaccgc
     5161 gaacgcgatc gacgtcagca gcaccagccc ttcgggcacc atcggcacca gcgcgccgac
     5221 catccgcagc acggattcgc gccatcccgc gccggtggtg aacagctggg tgtagatgat
     5281 cagcagcccg gccgggatca gcagatatgt gatgaaccgc aagatcgtgt tgatgccgct
     5341 gcgcagttcg gatttcacga gcgtgaactt gctggcctcc tcggccagtt tggcggcgta
     5401 tgcttcgtgg ccgaccctcg tcgcccggta ggcgccgctg ccggcgacga cgaagctgcc
     5461 cgacatcgcc gggtcgccga tgtccttggc gatcgagtcg gcctcaccgg tcagcagtga
     5521 ctcgtcgacc tccaagttca cgtcctcgac cacagtgccg tcgacgacga tctggtcgcc
     5581 cggacccaac tcgatgacgt cgtccagcac tacttcgctg ggcagcagtt cgcgtgttcc
     5641 gccttcgcgg cgcaccagcg gcttggcctg accgacgatc gcgagcttgt ccagtgtctg
     5701 cttggcgcgg atctcctgga tgatgccgat tccactgttg gcgatgatca gcagaccgaa
     5761 cagcccgttg atcaatgagc cggtggccag cacgatgacg aacagcaccc cgaggatcgc
     5821 gttgatccgg gtgaacacgt tggcgcggac gatctcggac acggtgcgcg ccgcccgggt
     5881 gggtacgtcg ttggtcttgc cctcggcgac ccgctgtgcg acttcggcgt cggtgagccc
     5941 ggtcgccagc gccggagccg cgggatccgt catggcttgc agagtagcca ctcagcgcgt
     6001 gaacgtgccg agcttgtcag tgtcgaagta ctccaacgtc aacgtgttcc cgttgaacgt
     6061 tgccttcgag atcgttcccg gcggcgcatt ctcgctctgc acggtgaacg tgaacgtgtc
     6121 gccgtcccag tgcgacaacg gaaacttgtc gccgcgcggg ccgatcgaca actccagcgc
     6181 gccattgact tcggcgactt tcgccggacc ccaatacggg ttggcgtatt cgccgacgta
     6241 cgcgttgagt ggcctggccg gggccgggtc ggcgggcgtc tgcttgccga caagcgagcc
     6301 ttcgggtttg tccatctcag cgaagacctg ccggtacagc gcgggccagt cctcccggat
     6361 cccgccgtac tgcacgagat ccatgaactg cgcgcccagc gtctcgggaa caccgattgg
     6421 cgcggcgttg gtcagcgcga tcagcccgac gttcgcggac ggcaacgccg cgaacgctgt
     6481 cgcggcgccc agcccgaacg cacccgaatg gctgaactgg gtgcggcccg acgacgtcac
     6541 cgacacgttg aagccgtggc cgtagaacga cgcgcgcgcg ttagggttcg ctgcgggcgc
     6601 ggagatcacc tgcggagtca cggtgggcag caacgcttcc ggcgacgcga tccgcacgcc
     6661 gttgtaggtg ccgttgccga gcacgagcgt cagccagcgg gccatgtcgt tgatcgacga
     6721 actcacacca ccggccggtg actgcgcgtc ggggtctcgg gtgtagagcg gctggtaccc
     6781 gtcgggtgtt ttgacgtggc ccacggcgcg attcgcgcgg gcttggaagt cggcgaaccg
     6841 cgaactcgtc gacgtcatgc ccaaaggccg gtacagcacc tcgtctgaga gcacgtccca
     6901 cggctttccg gctgcggcag cgacggcctc ggcgccggcg gtgagcccga agttggtgta
     6961 ctcgtacgta attcggaacg gggccagcgg cagatacctc agccgttcca gcacctgccg
     7021 ccggtcgtag ccgaggtctt cgaggatgtc accggcgtgg tcgggcaggc cggaccggtg
     7081 cgagtacagg tcggcgatcg tcgcggtgcg ggtgacgtag gggtccgcca gctcgaacca
     7141 gggcagcttg gcagacactg gtgtgtccca gccgatcgcg ttcgcggtca cctcgtgggc
     7201 caccacggtc gcccccatgg gtttcgacag cgacgcgagc tggaatacgg tgtcggcgtc
     7261 gaccttggcg cccgtccgca catcgctgac gccaaaccct ttgacgtaag ccacctttcc
     7321 gtcgtgcacg accgcaacgg ccatgccagg gatgcccgag ctcttcatca agtcgtcgac
     7381 gaggccgtcc agtttgccga tggcatcgtc gacccggccg gccggaatgt cgatgccgga
     7441 cacctcgttc ggcgggacgt cggacgatgg cgacgcagaa cgctgcggcg acccgcacgc
     7501 cacgagaagc aggactgagg cgagcgccgc tagccgtcgc cgagttctgc accagggctg
     7561 tggtttccgc gggatcacga ccgtcgtgca gatttcggcg gcgctcacag ccgccaccag
     7621 gcgtccaacg tggagggctc cgataaatcg atgcggtggc cgggcttggg caccgcgacg
     7681 ccgacaccgg ctgaacctgc ggcgtcgaca agccggtcga cgggctccgc ccagggatgc
     7741 ggcgcaagcc ggaacgtgcc ccagtgaatc ggcacgagaa gacccttgcc gggcccgctg
     7801 acgtcgaggt gggcgcggac ggcctcctcg ggattcatgt gaatgtctgg ccacagcctg
     7861 ttgtatgcgc cgacaggcat cagcgtcaga tcgaacggcc cgtgctgcga accgatttcg
     7921 gcgaagctcc tggtgtagcc ggtgtcgccg ccaaagtagg cgcggtgctg cgggccggtg
     7981 atcacccacg acgcccacag cgtggtgttg cggctgacga accggccgga gaagtgccgc
     8041 gccggcgtgc acaccaatgt caggtcgtcg acggaagcgc tttggtccca gtccaattcg
     8101 atgatccgct gctggggaat gccccaggtg cgcaggtgag tgccgacccc gagcgggacg
     8161 acgaacggcg cgcgctgact gcgggcgagc gccaacaccg tgtcgaggtc gagatggtcg
     8221 tagtggtcgt ggctgatcac cacggcgtcg acggccggca acgcctccaa cgggatcggc
     8281 ggcggatgca gccgctccgg tccgacggtg tgcgatggcg agcaccggtc accccagacc
     8341 gggtcggtga gcacgcggta cccgtcgatc tccagcaggg aggtggagtg gccgaaccag
     8401 gtgacggcca gcggtgccgg cggcatgccg aggccggctg gcttcacgac gggaatctca
     8461 ccggatggcc ggcccgcacg gccgcccagc atctcgcgca gcagaatccg ctgctcttcg
     8521 cggttgactt cgatcatcga ggcggggtcc acgttgacga acctgccgtc ccgatagttc
     8581 ggcgacccgt cggccacctc acgaatggcc agcggactgg cgcccaacgc cgcgggggcg
     8641 ccgcgcagcg cgcgcagcag ccatccgccc cctagcagcg tggcggtccc gatgccgact
     8701 cgcagcgcgc cccgcagcat ctcggctcag gcgcccttga agttgggttg ccgcttctgc
     8761 atccgcgcca cctgcgcctc gatgacgtcc tggctggccc acgccctttc gaacagctct
     8821 ttgtgaaccg gcgactggtc ctcgtaggcg ccgtcgtcgt tcagcacgcg cttcgcgtga
     8881 cgcaatgcca gcggcgccag cccggcgatc tcctcggccc acttctgcgc gtcggccagc
     8941 gagccgatcc ggttggccat gcctgtttgc agcgcgacgt cggcggtgag cttctccgcg
     9001 gtcagcagca tcgagcgggc gcgcccgtga ccggccagcg acgtcaaccg ccggatactc
     9061 caattgtcaa gcgccaggcc gtatttggcg gttggaaatt ggaagaacgc gtccggtgac
     9121 accacgcgga ggtcacagat catcgccagc tgcagtcccg cgccgatcgc cggaccgttg
     9181 atcgcgccga tcaccggcat cggcgcttcg tcgatggcct tgttcagcgc gatcagtcgg
     9241 tccgggtagt cctccgcgaa cgcgtcgccg gacaggtcgg cgcccgcgca gaacacgctg
     9301 ccctgtccgg tcagcacgac ggcacggatg tcgtcggcca ccgcctgcac gatcgccttg
     9361 cgcaaaccct cgacgagctc ggagttcagc gcgttgcgcc ggtcgggccg ctgcatctcg
     9421 atggtcgtga cgtggtcgac tcgggtgaca ccgatcatgg ggccacccta tccggggggc
     9481 gctggctacc ctcagttcgg tgagccggat ccccgccacc gtgctgcgtg acgcggtgct
     9541 cgacgctggg tcgttcgtca gctgggacac cgctcccctc gacgtggcgg ccgacgacga
     9601 ctacgcccgc gacctcgcgc aggcccgggc gtcgacaggg ttggacgagt cggtgatcac
     9661 cggagagggc acggtgcatg gccggcgcgt cgcggtcgtc gcatgcgagt tcgacttcct
     9721 cgccggctcg atcggcgtgg ccgcggccga acggatcacc gcggcggtgc agcgcgccac
     9781 ccgcgaccgg ctgcctctgc tggcgtcgcc gagctcgggc ggtacccgga tgcaggaggg
     9841 cacggtcgcg ttcttgcaga tggtcaagat cgccgccgcg gtgcaacagc acaagcaagt
     9901 gcatctgccc tacctggtgt acctgcggca c
//
LOCUS       DASWBF010000300         6162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_876488,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000300 DASWBF010000000
VERSION     DASWBF010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876488"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..2908)
                     /gene="topA"
                     /locus_tag="VF874_10855"
     CDS             complement(143..2908)
                     /gene="topA"
                     /locus_tag="VF874_10855"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX9176548.1"
                     /translation="MADDGRTSGNGNARVRRLVIVESPTKARKLAGYLGSNYVVESSR
                     GHIRDLPRAAADVPAKYKSEPWARLGVNVDRDFEPLYIISPDKKSTVSELKSKLKDAD
                     ELYLATDGDREGEAIAWHLLETLKPRIPVKRMVFHEITEPAIRAAAEHPRDLDSDLVD
                     AQETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVQRERDRMAFRSASYWD
                     VVAELDAGASATPRSFSARLVSVDDRRVATGRDFDSLGHLKNDVLVLDADSAGALARG
                     LRGAELTVSSVEQKPYTRKPYAPFMTSTLQQEAGRKLRFSSERTMSVAQRLYENGYIT
                     YMRTDSTTLSQSAIDAARRQARELYGDEYVHPTPRQYTRKVKNAQEAHEAIRPAGESF
                     ATPGQLHARLDNDEFRLYELIWQRTVASQMADARGTTLSLRITGQSSDGQSVALSASG
                     RTITFPGFLSAYVESLDDLAGGEADDAERRLPQLQQGQHVTATELTADGHTTNPPPRY
                     TEASLIKALEDLGIGRPSTYSSIIKTIQDRGYVFKKGSALVPSWVAFAVTGLLEQHFG
                     RLVDYDFTAAMEDELDEIANGQEQRTVWLTNFYFGGEHGVPESIARAGGLKKLVGVNL
                     EGIDAREVNSIKLFDDDEGRPIYVRVGKNGPYLERMIVGSDGEPTPQRANLSDSITPD
                     ELSLELAEKLFATPQEGRSLGVDPETGHEIVAKDGRFGPYVTEVLPTPPDDDGDGVAA
                     KKGKKPTGPKPRTGSLLRTMDLETVTLEDALRLLSLPRVVGVDPSTGEEITAQNGRYG
                     PYLKRGNDSRSLATEEQMFTITLDEAVKIYSEPKRRGRQAASLPPLRELGNDPATGKP
                     MVIKDGRFGPYVTDGETNASLRKGDDVLTITDQRAAELLADRRARGPVKKRAAKKGAR
                     KRPAKKA"
     gene            complement(3022..3600)
                     /locus_tag="VF874_10860"
     CDS             complement(3022..3600)
                     /locus_tag="VF874_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176549.1"
                     /translation="MSQLSFFSAESVPPAVTDLTGVLAAPGQVVLVGTGARLSVVVER
                     PWRAEALAEMMDDAGLEPEITRTDEDTPLVRTSVDSRLVSIADDWTRGAVKTVPRQWL
                     PGPRELRAWVLAAGTPEADRYLLGLDPHAPDTHSPLASALMRVGIAPTLIGTRGARPA
                     LRISGRRRLSRLVENVGEPPDDVEALGQWPHV"
     gene            complement(3762..3965)
                     /locus_tag="VF874_10865"
     CDS             complement(3762..3965)
                     /locus_tag="VF874_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9176550.1"
                     /translation="MPQGTVKWFNAEKGFGFIAPEDGSADVFVHYTEIQGNGFRTLEE
                     NQRVEFEVGQSPKGPQATGVRSI"
     gene            4230..4663
                     /locus_tag="VF874_10870"
                     /pseudo
     CDS             4230..4663
                     /locus_tag="VF874_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113549.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4669..>6162
                     /gene="tnpB"
                     /locus_tag="VF874_10875"
     CDS             4669..>6162
                     /gene="tnpB"
                     /locus_tag="VF874_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family element RNA-guided endonuclease
                     TnpB"
                     /protein_id="HEX9176551.1"
                     /translation="MAGVEIPDGWVAQGYRFALDPTPRQVEELLSHAGGARFAYNVML
                     AAVKANLDQRAAERSCGIAEDDLTPCMSWSFVSLRNDWNQRKHTVAVRADGTPWWPGN
                     SKEVYANACRALADALSHWADSRKGARKGPRMGFPHFKTKTRAVKKFSFTTGTIRVDP
                     DRHHITLPRLGTIKTHESTRKLARRIQTGTARILKATVRLERGRWLVALTCIVARQTG
                     RPAHVKPGAAVVGIDAGVKDLIVVADPAGNELARHRAPRELKQAQSKLRALQRKAARQ
                     VGPWDPTAQRRQDPSQGWLRTQRQIGRAHARVANLRADRIHKLTTALSQTHDVIGAET
                     LAVKNMMAGGGAGKRGLNRALGDAGLGVLLRQLDYKTGWYGSRVLKADRWFPSSKLCS
                     GCGVVKARLHLAERTYECDSCGLTIDRDLNAAVNLARYAQQQTDPSTGVVTGGADLKP
                     SPPGDANGNETRTRAEVGDNADDGGSAPPKRRLPEMPVIQNTLTFQVT"
BASE COUNT         1008 a         2041 c         2028 g         1085 t
ORIGIN      
        1 gcgggcggac ggcgcccact cagctcgcac ggccggtgaa actcaatgtc ccgtcggagg
       61 tttgaagctg cgccgaggct gcaaatcagc agggaaagat cgagtagacg ccctgtgaaa
      121 atacagtttc ggcgcaatga ggttaggcct tcttggccgg cctcttgcga gcgcccttct
      181 ttgcggcccg cttcttcacc ggcccgcggg cgcggcggtc ggccagcagc tcggcagcac
      241 gttggtcggt gatcgtcagc acgtcgtcgc ccttgcgcag gctggcgttg gtctcgccgt
      301 cggtgacgta cgggccgaac cgcccgtcct tgatcaccat cggcttaccc gtcgccgggt
      361 cgttgcccag ctcgcgcagc ggcggcagcg aagctgcttg tcgcccacgg cgtttgggct
      421 ctgagtagat cttcacagcc tcgtcgagcg tgatcgtgaa catctgctct tcagtcgcca
      481 gcgaccgaga gtcgttgccg cgcttcagat atggtccgta gcgaccgttc tgtgcagtga
      541 tctcctcgcc ggtcgacggg tcaacgccca ccacccgggg cagcgacagc agccgcagcg
      601 cgtcttccag ggtgacggtc tccagatcca tcgtccgcag cagcgacccg gtgcgtggct
      661 tcgggccggt tggcttcttg cccttcttgg cggccacgcc gtcgccgtcg tcgtcgggcg
      721 gcgtcggcag tacctcggtg acatacggac cgaagcggcc gtccttggcg acgatctcat
      781 gcccggtctc cgggtcgaca cccagcgatc ggccctcttg cggtgtggcg aacagctttt
      841 cggccaactc gaggctcagc tcgtcgggag tgatcgagtc ggacagattc gcccgctgcg
      901 gcgtcggctc accgtcggat ccgacgatca tgcgctccaa atacggcccg ttcttgccga
      961 cgcggacata aatcgggcgt ccctcgtcgt cgtcaaagag cttgatggag ttcacttctc
     1021 gcgcatcgat gccctcgagg ttgaccccga ccagcttctt cagtccgccc gcacgtgcga
     1081 tcgactccgg tacgccgtgc tcgccgccga agtagaagtt ggtcaaccac accgtgcgct
     1141 gctcttggcc gttggcgatc tcgtcgagct cgtcttccat cgccgcggtg aagtcgtagt
     1201 cgaccagccg gccgaagtgc tgctcgagca gacccgtcac cgcgaacgcc acccacgacg
     1261 gcaccagggc actgcccttc ttgaacacgt acccacggtc ctggatggtc ttgatgatcg
     1321 acgagtacgt cgacggccga ccgatgccga ggtcttccag cgccttgatc agcgacgcct
     1381 cggtgtagcg aggcggcggg ttggtggtgt gcccgtcggc ggtcaactcg gtcgccgtga
     1441 cgtgctgacc ctgctgaagc tgcggcagcc ggcgctcggc gtcgtcggct tcaccgccgg
     1501 cgaggtcgtc gaggctttcg acgtacgccg acaagaagcc cgggaacgtg atcgtgcgcc
     1561 cgctggcgga caacgccacc gactggccgt cggacgactg gccggtgatg cgcaagctca
     1621 gcgtggtgcc gcgcgcgtcg gccatctgcg aggcgaccgt gcgctgccag atcagctcgt
     1681 agagccggaa ctcgtcgttg tcgagtcgcg cgtgcagctg ccccggggtg gcgaaagatt
     1741 ccccggcggg ccggatcgcc tcgtgcgcct cctgcgcgtt cttgaccttg cgggtgtact
     1801 ggcgcggcgt gggatgcacg tactcgtcgc cgtacagctc gcgcgcctgc ctgcgcgcgg
     1861 cgtcgatcgc cgactgcgac agcgtggtcg agtcggtccg catgtaagtg atgtaaccgt
     1921 tctcgtatag ccgctgcgcg acgctcatcg tgcgctccga gctgaaccgc agcttgcggc
     1981 ccgcctcctg ctgcagcgtc gacgtcatga acggcgcata cggcttgcgg gtgtagggct
     2041 tctgctccac cgacgagacg gtcagctcag caccacgcaa ccctcgcgcc agcgctccgg
     2101 cgctgtcggc gtcgagcacc agcacgtcgt tcttcaaatg gcccagcgag tcgaagtcgc
     2161 ggccggtggc aacacggcgg tcgtcgacgc tgaccagccg cgccgagaag ctgcgcggcg
     2221 tcgccgacgc gcccgcgtcc agctcggcca ccacgtccca gtagctggcg ctgcggaacg
     2281 ccatccggtc gcgttcgcgc tggacgatga tccgcgtggc caccgactgc acccggcccg
     2341 ctgacagctt gggtgcgacc ttcttccaca gcaccgggct gacctcgtag ccgtacagcc
     2401 ggtccaggat gcggcgagtc tcctgcgcat cgaccaggtc gctgtccaag tcacgcgggt
     2461 gctcggcggc ggcacggatc gccggctcgg tgatctcgtg gaacaccatc cgcttgaccg
     2521 gaatgcgcgg cttcagtgtc tccagcaggt gccaggcgat cgcctcgccc tcacggtcgc
     2581 cgtcggtggc cagatacaac tcgtcggcgt ccttgagctt gctcttcagc tcactgaccg
     2641 tgctcttctt gtccgggctg atgatgtaga gcggctcgaa gtcgcggtcc acgttcacgc
     2701 cgagccgcgc ccacggctcc gacttgtact tggccggcac atcagcggcg gccctcggca
     2761 ggtcgcggat gtggccgcgg gaggattcga cgacgtagtt ggagcccaga taaccggcta
     2821 atttgcgcgc tttggtcggc gactcgacaa tgacgagccg tcgtacacgt gcgttcccgt
     2881 tgccgctcgt gcggccgtcg tcagccaact gcgcttacgc tccacttctt cctgccgatc
     2941 ggcgccctgc gggcctgtca gcacctgaca agatgacacc ccgagcgtgc cgacgcaaac
     3001 tggctccccc gtcaactccc ctcagacgtg aggccactgc cctaacgcct cgacgtcgtc
     3061 gggcggttcc cctacgttct ctaccaggcg tgatagccga cggcggccgc tgatccgcag
     3121 tgcgggacgg gctccgcggg tgccgatcaa tgtcggcgcg atcccgaccc gcatcagcgc
     3181 cgacgccaac ggggagtggg tatccggcgc gtgcggatcc aaacccagca agtagcggtc
     3241 cgcttcgggg gtgcccgccg cgaggaccca ggccctcagc tcgcgtgggc cgggcagcca
     3301 ctggcgcggc acggtcttga cggcgccgcg cgtccagtcg tcagcgatgc tgaccaggcg
     3361 cgagtccacc gacgtgcgca ccagcggagt gtcctcgtcg gtgcgggtga tctcgggctc
     3421 gaggccggca tcgtccatca tctcggccag cgcctcggcg cgccacggac gttcgacgac
     3481 cacggacagc cgcgctccgg tgccgaccag caccacctga ccgggggcgg ccagcacgcc
     3541 ggtcaagtcg gtcaccgcag gcggcaccga ctcggccgag aagaaggaca gctggctcac
     3601 gtcacgacag taagcccgct cggatcgccg cgagcgtgcg cgtagtgcca acctgcgcgg
     3661 cgtgtcgctg gacaaacatg cacgctcgca cgaagaggag gcgagcaaga gaaaaccccc
     3721 gcaggtcgag acactgcggg ggttttgtca ggtctggcta ctcagatgga acgcacacct
     3781 gtggcctgcg gccccttcgg gctctgcccg acctcgaact caactcgttg gttctcctcc
     3841 agggtgcgaa acccattccc ttgaatctcc gtgtagtgga caaacacgtc agcagatccg
     3901 tcctccggcg cgataaagcc aaagcctttc tccgcgttga accacttcac agttccctgt
     3961 ggcatttctc gatgtttcct tctcttacag ggttgcggtg caccgtcttt cggtacaccg
     4021 ggcccgttcc gaccgctaat ccttcgtggt gtcgccgatc tcaaccgact tgccatcctc
     4081 gcaggaaccg cgaccgcaac gtcgatcctg cgagtgcaat aacacgaaca cagaagctgc
     4141 gaccgtggtc agtcaatcat gttcgccgca attgcaacag tcttgggcgc ctgaactcat
     4201 gaacaattca cctggcggag atgtcgacat atgtccgggt gcgcagccat gatgagcggg
     4261 ccgatttgga ttggcgggtg tcgtggttga ccgggtgggc gaggaccaat ggtttggcat
     4321 ttgggcaggt ggttgccgag ggtcggttcc gggctgaacg gcaaccgcgc gaggtttggc
     4381 ccgggtgttg tcagacccat ctgccagggt gattgttgtg aagcatcgag gtcggctggc
     4441 gcgttgtggg tggcgcatct gcaagccgcg ctggcggggc agggtcgcca ggttgtggtc
     4501 gtcgacagcg gtgacacgcc ccgatgatgc ggtgcgcgac atggtcgatg ttctgaggtc
     4561 gatgtgcgcc tgctggtata ggcgtggcgg tgcgcggcac cgggcgttgc gtgcggtaac
     4621 tgctgttcgg cggcagccgg acgcggccga ggctatggga tagaggtggt ggcgggggtt
     4681 gagattcccg acggctgggt ggcccagggg taccggtttg ccctggatcc gacacccagg
     4741 caggtcgagg aattgctgtc gcatgccggt ggggcgcggt ttgcctacaa cgtgatgctg
     4801 gcggcggtga aagccaacct ggatcaacgc gccgccgagc gctcctgtgg gatcgccgag
     4861 gacgatctga cgccgtgcat gagttggtcg tttgtgtcgc tgcgcaacga ctggaaccag
     4921 cgcaagcaca ctgtcgcggt gcgtgccgac ggcacgccgt ggtggccggg caactccaaa
     4981 gaggtgtacg ccaatgcgtg ccgggcgctg gccgacgccc tgtcgcactg ggccgactca
     5041 cgaaaaggtg cccgtaaggg tccgcggatg gggttccccc acttcaaaac gaaaacccgt
     5101 gcggtcaaga agttctcgtt caccactggc acgattcggg tcgacccgga ccgtcaccac
     5161 atcaccttgc cccggctcgg gaccatcaag acccacgaga gcacccgcaa gctggcccgc
     5221 cggatccaga ccggcactgc ccgcatcctc aaggcgaccg tgcgcttgga gcggggccgc
     5281 tggctggtgg cgttgacttg catcgtggcg cgccagaccg gtcggccggc gcatgtgaaa
     5341 cccggtgctg cggtggtggg catcgacgcc ggggtcaagg acttgatcgt ggtcgccgat
     5401 cccgccggca acgagctagc ccgccaccgc gcgccgcgcg agctcaagca ggcgcaatcc
     5461 aaactgcggg cgttgcagcg caaggcggct cgccaggtcg gcccctggga cccgacggcc
     5521 caacgtcgcc aggatccgtc gcaaggctgg ctgcgcaccc aacgccagat cgggcgcgcc
     5581 catgcccggg tggcgaacct gcgcgccgac cgcatccaca agctcaccac cgccctgagc
     5641 caaacccatg acgtgatcgg cgccgagaca ctggcggtga agaacatgat ggccggcggc
     5701 ggggcaggca aacgtggcct caaccgtgca ctgggcgacg ccggcctggg cgtgttgttg
     5761 cgacaactcg attacaagac cggctggtat ggctcacggg tcctcaaggc cgatcggtgg
     5821 tttcccagct cgaaattgtg ctcgggctgc ggtgtggtga aagccaggct gcacctggcc
     5881 gagcggacat atgaatgcga cagctgtggt ctcacgatcg atcgggatct caacgcagcg
     5941 gtcaacctag cgcgctacgc gcaacaacaa acggacccga gtaccggggt cgtcacgggc
     6001 ggagccgacc ttaagccgtc acctcccggt gacgctaatg gcaatgaaac ccgaacccga
     6061 gccgaagtag gcgacaacgc cgatgacggt gggagtgccc ctccaaagag aaggctgcct
     6121 gaaatgccag tcatacaaaa tacactcaca tttcaggtaa cg
//
LOCUS       DASWBF010000301         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1076543,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000301 DASWBF010000000
VERSION     DASWBF010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076543"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..2480)
                     /locus_tag="VF874_10880"
     CDS             complement(153..2480)
                     /locus_tag="VF874_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9176552.1"
                     /translation="MTVVTSVQSAAADRGRLSGHLLRDGRWDRRLGLLDGEAAALAAL
                     GPVGLRRNWGVPQKTAPQWNALARLVEPLDEAVAALHHGEHVRYRRAGAHAAGVVLQQ
                     CADSGRSWWGWAPWEWARLCGTSAGEFLTTQTPPTESTVRPYIVALAYLLGGFTDFHR
                     LGTFNRLHLACLIFGEPAVDESLRRAGEVLDRWGYRDSTGVTHRMRGVFSQALLLNHS
                     PRLDDLDTAAFAALRAHPASDGHQGSMLYALQRVVAALGHCDPPVRTGYNHAAGIEGT
                     DPAWAAWIERWYATSALTPKVRAMIRTITAKSGRWLAAEHPEITQPGQWTRATCAAWV
                     AAVDRMSVGDWVQRREGLGARVGEPISPRTKAQNLVATRTFFRDCQEWEWIGRRFDPN
                     RALAVPRSIAALIGTNPRVIADDVWAKLLWAGLNLQPADLPGTYTYYPMELIRAVTLT
                     WLFSGLRSDEITRLRVGCIRWQHTGLPIAGDSREVLAEDAVCLLDVPVHKTGTAFTKP
                     VDPIVGQAVQAWQVLRPEQPKRLDRKTKEHVHMLFSVRAQPVAKVYINHTIIPALCAK
                     AGVPTADVRGKITSHRARSTIASQLYNAKEPMTLFELQAWLGHRTPTTTQHYAKITPN
                     TLSKAYTQAGYFARNVRTIEVLIDRDAVTSGASAAGQPWQHYDLGHGWCTYSFFEQCP
                     HRMACARCDFYIPKGSTKAQLLEASDNLQRMLASIPLTDDERAAVDDGHTAVTTLLDR
                     LADVPTPAGPTPRELGTPATVTMLPIVQIRTGKSS"
     gene            complement(2477..3508)
                     /locus_tag="VF874_10885"
     CDS             complement(2477..3508)
                     /locus_tag="VF874_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9176553.1"
                     /translation="MLTRHDRALEWLEIWIDLGRAPRTIDAYGRGLAEFLQVCEREGI
                     DPLSATRAQVAVFVRELTSRPSRRGTNVVALDSGSGLSNATLQQRLVPVRLFFDFLVE
                     EGVRESNPVGRGRYTPGRAFGGRAGALVQRMVKLPWIPSEADWLRLLGVVAEEPIRNR
                     VMFALAYDAALRREELCALRSDDLDPAHRTLRVRAETTKTRRERMVPYSAPTGVLLAK
                     YLAHRATLSRARGGLFLSESHRNHAQPVTLWTWSKVVRRIALAANLPRFSTHTTRHLC
                     LTDLARMGWELHAIATFAGHRSTDSTLQYIHLSGRDLSAKLNSSMVQIHAWRIGMLTG
                     PEVSGQDPP"
     gene            complement(3641..3766)
                     /locus_tag="VF874_10890"
                     /pseudo
     CDS             complement(3641..3766)
                     /locus_tag="VF874_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          624 a         1281 c         1261 g          600 t
ORIGIN      
        1 caatgctgct cgacgaaatg gctgccctag accgccgcag cggcacgagg tggcgaaggt
       61 actccggaca atggctgaca cgttcgccga ggtgcccgac ggtcggaata ccacacacac
      121 acatacattc tgtggaatca aaattgtcaa gttcacgagg atttcccggt tcggatctgc
      181 acgatcggca gcatggtcac ggtcgcgggc gtgccgagct cacgcggggt gggtccagcg
      241 ggagtcggta cgtcggcgag ccggtcgagc agtgtggtga cggcggtgtg tccgtcgtcg
      301 acggcggcgc gttcatcgtc ggtgagcggg atgctggcga gcatgcgctg caggttatcg
      361 cttgcctcga gcagctgggc tttggtcgag cccttgggga tgtagaaatc gcaacgagcg
      421 caagccattc ggtgcgggca ttgttcgaag aagctgtagg tgcaccagcc gtggccgagg
      481 tcgtaatgct gccagggttg gccggcggcg gatgcaccgg aggtgacggc gtcgcggtcg
      541 atgaggacct cgatggtgcg cacgttgcgg gcgaagtatc cggcttgggt gtaggccttg
      601 gacagcgtgt tcggggtgat cttggcgtaa tgctgggtgg tggtcggggt gcggtggcct
      661 agccaggcct gcagctcgaa cagcgtcatc ggctccttgg cgttgtagag ctggctggcg
      721 atggtggagc gggcgcggtg gctggttatc ttgccgcgga cgtcggcggt ggggacgccg
      781 gccttggcgc acagcgccgg gatgatggtg tggttgatgt agaccttggc cacgggctga
      841 gcgcgcaccg agaacagcat gtgtacatgc tctttagtct tgcggtccaa ccgtttcggc
      901 tgttcgggtc gaagcacctg ccacgcctgg acggcttggc cgacgatcgg gtcgacgggc
      961 ttggtgaacg cagtgccggt cttgtgcacc ggcacgtcga gcaggcagac cgcgtcctcg
     1021 gcgaggacct cgcgtgagtc gccagcgatg ggcaggcccg tgtgctgcca gcggatgcat
     1081 cccacccgca gtcgggtgat ctcgtcgctg cgcagcccgg agaacagcca ggtcagtgtg
     1141 accgcgcgga tcaactccat cggatagtag gtgtaggtgc cgggcagatc ggcgggttgc
     1201 aggttcagcc ccgcccacag cagcttggcc cacacgtcgt cggcgatgac gcgggggttg
     1261 gtgccgatca gggcggcgat gctgcgtggc acggccaatg cccggttcgg gtcgaaccgg
     1321 cggccgatcc attcccattc ctggcagtcc cgaaagaacg tgcgagtggc cacgaggttt
     1381 tgcgccttgg tgcgcggcga gatcggttct cccacacggg cgccgagtcc ctcgcgacgc
     1441 tgaacccagt cgccgacgct catccggtcg acggcggcga cccaggcggc gcaggtcgcc
     1501 ctggtccact ggccgggttg ggtgatctcg gggtgctcgg cggccaacca ccgtccgctc
     1561 ttggccgtga tggtgcggat catggcgcgc accttcgggg tcagtgccga ggtggcatac
     1621 cagcgttcga tccaggcggc ccacgccggg tcagtgccct cgatgccagc agcgtggttg
     1681 taaccggtgc gcaccggtgg atcgcagtga cccagcgcgg cgaccacgcg ctgcagcgca
     1741 tacagcatcg acccctggtg cccgtcgctg gccggatgcg cccgcagcgc cgcgaacgcc
     1801 gcggtgtcca gatcgtcgag ccgcggactg tggttgagca gcagggcttg gctgaacacc
     1861 ccccgcatcc ggtgcgtgac accggtgctg tcccggtatc cccaccggtc gagcacctcg
     1921 ccggcccgac gcagcgactc gtcgacagca ggttcaccaa agatcaagca ggccaagtgg
     1981 agccgattga aagtgcccag ccggtgaaag tcggtgaacc cgccgagcag gtaggccaaa
     2041 gccacgatgt acggtcgcac cgttgactcg gtcggcggtg tctgcgtggt caggaactcg
     2101 ccggcgctgg tcccgcacaa ccgcgcccac tcccacggcg cccagcccca ccagctgcgg
     2161 ccggagtccg cgcactgctg taagaccact cctgccgcgt gcgcgcccgc gcgtcggtaa
     2221 cgcacgtgct cgccgtgatg cagcgcggcg acggcctcat cgagcggttc gaccaagcgg
     2281 gccaacgcat tccattgtgg agctgtcttt tgcggcacgc cccagttacg gcgcagcccg
     2341 accggaccca gcgcggccag cgccgcggcc tcgccgtcga gcagcccgag ccggcgatcc
     2401 cagcgaccgt cccgaagaag atgcccagac agtcggccgc ggtccgcggc ggccgactgt
     2461 accgacgtga cgacggtcat ggggggtcct gtccgctgac ttcgggtccg gtgagcatgc
     2521 cgatccgcca cgcatggatc tgcaccatcg aggagttcaa cttcgccgac aggtcccgcc
     2581 cggacaggtg gatgtactgc agcgtggagt cggtgctgcg atgcccggcg aacgtggcga
     2641 tcgcgtgcag ctcccagccc atccgggcca gatccgtcaa gcacagatgc cgcgttgtat
     2701 gggtggagaa ccggggaagg ttggccgcca acgcgattcg ccgcaccacc ttcgaccacg
     2761 tccacaacgt gacgggttgg gcgtggttgc ggtgcgactc cgacaggaac aaaccgcccc
     2821 gggcccggct cagcgtcgcc cggtgcgcca ggtacttcgc cagcagcaca cccgtgggcg
     2881 ccgagtacgg caccatccgc tcccggcggg tcttcgtggt ctccgcacgc acccgcaacg
     2941 tgcggtgcgc cgggtcgaga tcgtcgctgc gcagcgcgca cagctcctcc cggcgcaacg
     3001 ccgcgtcata ggccagcgcg aacatcaccc ggttgcggat cggctcctcg gccaccacgc
     3061 ccaacaggcg cagccagtcg gcctcactgg ggatccacgg cagcttcacc atccgctgca
     3121 ccagcgcacc ggcccgacca ccaaacgctc gacccggcgt ataacggccc cggccaacag
     3181 gattcgactc acgcacgcct tcttcgacca ggaagtcgaa gaacagtcga accggcacca
     3241 gacgctgctg cagggtggcg ttggacagcc ccgaccccga gtccagcgcc accacgttgg
     3301 tgccgcgccg actcggccgc gacgtcaact cccgcacgaa caccgccacc tgcgcccggg
     3361 tcgcggacag cgggtcgatc ccctcccgct cacagacctg cagaaactcc gccagccccc
     3421 gcccatacgc atcgatagtt cgcggggcac gccccagatc gatccagatc tccaaccact
     3481 ccaatgcccg atcgtgccga gtcaacaccg gccacttcgt ctccaacgcc ccagacacgc
     3541 ccaaccccat atgtcacccc gacccccgct gaacagtgac gctacgaggc cgaaccgcgc
     3601 tccccgccga ccgacacgcg gtctgattcc acgtaactta taaataccgg tgccccttgc
     3661 ggtaggcgat ctcgtcgatc ccgatccggg acagcccatc cagcaggtcg ttggtgtcgc
     3721 ggccatcggc caccaccctg gtcacgatcg ccgccacggt gcgcca
//
LOCUS       DASWBF010000302         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_876539,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000302 DASWBF010000000
VERSION     DASWBF010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876539"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2146
                     /locus_tag="VF874_10895"
     CDS             <1..2146
                     /locus_tag="VF874_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9176554.1"
                     /translation="IDWVEAARGRCDPRALDDLWANVPEPMRLSRFAESSVPPEFAGA
                     FCHDGTWRAGVDLSELPEPMRREVAWCVFRIIELGGKIPTPGLSMLVRRLGEVIADRA
                     GRAPASLLGVSVPEWCQQIQRAVHRRSGRLPAATTMTTIRRLLTRMMRLLVTASDTGP
                     WWQRDQWNPVEDNRIPLRDHEPMGRYSVRFDRIGTRWLRRGVQWHCKVGLDTGVLSWS
                     TVHRRIVAVGEFDGFLARRGVDGPWLADHAAGIRALMLDFLGHLRARPVTRGRRTGQR
                     LSPASVQRLASDVEQFYLFMTDNKDAAAAALAEPGWLRLGPEHAGFYRRGELPGKPRP
                     RLDGQVIDDDAMTRIMGGLDLLGAAVADGGFGDEQAMRITMLVALLGRRVSEICLLDR
                     DPLLPLSPTAPSSPGEPAAVGDDQALVAKLRYQQTKIDGAPDTILVPAEVVAIIREQQ
                     QWAQRFFAEHGAPGRTPKYLFLAAQMNRNGDRPYSGNTLRNLLTDLAIRLDVRDSTGA
                     LVDFNRTHRFRHTVATGLLNSGVPLHVLQRYLGHLTPTMSMTYAQTLQSTAEAEFLRY
                     RKITADGREIEVDPRDLYDMLELDRRTDRILPNGYCLLPPRQVCGKGNACLTCDKFAT
                     DATFLPELQTQPARTEQLITERRATFEARTGHQMGEDNIWLAGRRQERDALGRIILTL
                     KNTRLADGTVQAVRGAGVEARTEAITGKEDNT"
     gene            2146..2574
                     /locus_tag="VF874_10900"
     CDS             2146..2574
                     /locus_tag="VF874_10900"
                     /inference="COORDINATES: protein motif:HMM:NF040041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HEX9176555.1"
                     /translation="MRGNPDNLRQAAARKSAAAQARAEQGLHEIIRRNEPITFRGLAQ
                     TAGVSLDFLYRCTPIRQRVQQLRAQQQNTPPPAAATPTRDGTPSSVVRTLTAQLAELK
                     RRHRDEINALKQALEAAHGENLELRRRLGSHRARDDTTTP"
     gene            2821..>3018
                     /locus_tag="VF874_10905"
     CDS             2821..>3018
                     /locus_tag="VF874_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEX9176556.1"
                     /translation="MDLYQQGIPLPIIMRLLGQENASTTTAFYAFATLDMMRQAINAA
                     TPAINAHTTDPLTEDQLQTLYS"
BASE COUNT          533 a         1062 c          962 g          461 t
ORIGIN      
        1 gatcgactgg gtggaagcgg cccgcggccg ctgcgacccg cgggcgctgg acgacctgtg
       61 ggcgaatgtg ccggaaccga tgcggctttc acgcttcgcc gagtcgagtg ttccaccgga
      121 gttcgcggga gcgttctgcc acgacgggac ctggcgggcc ggggtggacc tgtcggagct
      181 gccggagccg atgcgccggg aggtggcgtg gtgtgtgttc cgcatcatcg agttgggcgg
      241 gaagatcccg acaccggggt tgagcatgct ggtgcgccga ctgggtgagg tgatcgccga
      301 tcgtgccggg cgcgcgccgg cctccctgct cggggtgtcc gtgccggagt ggtgccaaca
      361 gatccagcgt gcggtgcacc gccgcagcgg gcggctgccg gcggctacca cgatgaccac
      421 catccgccgg ttgctgacgc ggatgatgcg tctgctcgtc accgcctccg acaccggccc
      481 gtggtggcag cgggaccagt ggaacccggt ggaggacaac cggattccgc tgcgcgatca
      541 cgagccgatg ggccggtact cggtgcggtt cgaccggatc ggcacccggt ggctgcggcg
      601 cggcgtgcag tggcactgca aggtcggcct ggacaccggg gtgttgagct ggtcgacggt
      661 gcaccgccga atcgtggcgg tgggcgagtt cgacgggttc ttggcccgtc gcggggtcga
      721 tgggccgtgg ttggccgatc acgcggccgg gatacgcgcg ctcatgctgg atttcctcgg
      781 tcatctccgc gcccgtccgg tcacccgtgg acgccgcacc ggccagcggt tgtcgccggc
      841 gtcggtgcag cgcctggcca gcgatgtgga gcagttctac ctgttcatga ccgacaacaa
      901 ggacgcggcc gccgccgcgc tggccgaacc gggctggctg cgactcggac ccgagcacgc
      961 cggcttctat cggcgtggtg aactgcccgg caagccgcgg ccccgactcg acggtcaggt
     1021 catcgacgac gacgccatga cccggatcat gggcgggctg gatctgctcg gcgccgcggt
     1081 cgccgacggc gggttcggtg acgagcaggc catgcggatc accatgctgg tggcgttgct
     1141 gggccggcgt gtcagcgaga tctgcctgct cgaccgcgac ccactgctgc cgttgtcgcc
     1201 gacagccccg tcctcgccgg gagagcccgc ggcagtcggc gacgaccagg cactggtcgc
     1261 caagctgcgc taccagcaga cgaagatcga cggtgctccg gacaccatcc tcgtgcccgc
     1321 cgaggtcgtc gcgatcatcc gcgaacagca acagtgggcg cagcggttct tcgccgaaca
     1381 cggcgcaccc ggcaggaccc cgaagtatct gttcctcgcg gcgcagatga atcgcaacgg
     1441 cgaccggccc tactccggca acaccctgcg gaatctgctg accgatctgg ccattcggct
     1501 ggacgtgcgt gacagcacgg gcgccctggt cgacttcaac cgcacccacc ggtttcgcca
     1561 caccgtggcc accggcttgc tgaacagcgg cgtcccgttg catgtgttgc agcgttacct
     1621 cgggcatctc acgccgacca tgagcatgac ctatgcgcag accctgcagt ccaccgccga
     1681 ggccgagttt ctgcgctacc gcaagatcac cgccgatggg cgtgagatcg aggtcgatcc
     1741 ccgcgacctc tacgacatgc tcgagttgga ccggcgtacc gaccgcatcc tgcccaacgg
     1801 gtactgcttg ctgcctcccc gtcaggtctg cggcaagggc aacgcctgtc tgacctgcga
     1861 caagttcgcc accgacgcga ccttcctgcc cgagctgcaa acccagccgg cccgcaccga
     1921 gcaactcatc accgaacgcc gcgcgacgtt cgaggcccgc accggacacc agatgggcga
     1981 ggacaacatc tggctggccg gacggcggca ggaacgcgac gccctcggcc ggatcatcct
     2041 caccctcaag aacactcggc tcgccgacgg caccgtccag gcggtacgcg gcgccggcgt
     2101 cgaggcccgc accgaggcca tcaccggaaa agaggacaac acctgatgcg gggcaacccg
     2161 gacaacctcc gccaagccgc cgcccgcaag agcgccgccg ctcaggcccg agccgagcaa
     2221 ggactgcacg agatcatccg ccgtaacgag ccgatcacct tccgcggcct ggcccagacc
     2281 gccggtgtct ccctggactt cctctaccgc tgcaccccga tacgccaacg cgtccaacaa
     2341 cttcgggccc aacagcagaa cacaccgcca ccggcggcag cgaccccgac ccgggacgga
     2401 acgccgagca gcgtcgtccg caccctcacc gcgcagctcg ccgaactcaa acggcgtcac
     2461 cgcgacgaga tcaacgctct caaacaagcg ctcgaggccg cacacggcga gaatttggaa
     2521 ctacgtcgcc gtctcggctc ccatcgagcc cgagacgaca ccacgacacc ctgacgctcc
     2581 ccttgcagac gcgtacgcaa acgatctacc tgcgtaaaca cacaaattca agatcattac
     2641 cccagataat gacacggctt aacgctgatt atgccgaggt ttgcgttaag ccgaggattc
     2701 gtggagatcg cttgtgccgg tgggggttgc tggtcgattc ctcggcttaa cgtttcgggc
     2761 tgtggttagc gtacgtcgta tctctacgtc tgtggaagga gacggcgcaa gaccaaagcc
     2821 atggatctct accagcaggg catcccgctg ccaatcatca tgcgcctcct cggccaggaa
     2881 aacgcttcca cgacaacggc gttctacgcc ttcgcaaccc tggacatgat gcgacaagcc
     2941 atcaacgcag ccacccccgc gatcaatgcc cacacgaccg atccgctcac cgaagaccaa
     3001 cttcaaaccc tctacagt
//
LOCUS       DASWBF010000303         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1014020,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000303 DASWBF010000000
VERSION     DASWBF010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014020"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF874_10910"
     CDS             complement(<1..552)
                     /locus_tag="VF874_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX9176557.1"
                     /translation="MTLAKIRHTLRGLRFATVVSARQVSRRRIVATLIGVVILVAAVV
                     FVPMPTAVELRDWATSAGGWFPLAFLAAHVVVTVFPFPRTAFTLAAGLLFGTARGVTL
                     AVAASTLSALIALWLVRVAGWQLDRLVRHPGIEAVDARFRQRGWAIVLSLRLIPAVPF
                     SVINYAAGASAVRVSPYTVATFVG"
     gene            604..2451
                     /gene="mutA"
                     /locus_tag="VF874_10915"
     CDS             604..2451
                     /gene="mutA"
                     /locus_tag="VF874_10915"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HEX9176558.1"
                     /translation="MSTQARVDSAEVEGALSQWRSQVAAVLAKRSTDLPADPERMLDT
                     PTYEGFPVRALYTALDARPEDPLPGRWPFVRGGDALRDVNTGWKVAEAFPAHGGAAVG
                     DLNGAVLLALTEGVSALVLRVGDARVPPDALDRLLEGVFIELVPIVLDAGADLGAAAD
                     AVLALAATLDEDKYAMLSIDLGADPLTAALSGRAAPGLDEVVAFASRAAAYRGGVRAI
                     TVDGPVFHNRGANASWELAGSVAAGVAYLRSLPDLSVAQALRQISFRFAADDDQFMTI
                     AKFRAARQLWARVAEVAEAPGAGAATMHAVTSLPMMTQRDPWVNMLRTTVAAFAAGIG
                     GAETVLVEPFDAAIPGGAPGVSAGFSRRIARNTQLLLLEESNIGRVLDPAGGSWYVED
                     LTEALAQQAWSHFQAIESRGGFTDAHDFIAAHLQQVSDQRSDDVDHRRTKITGVNEYP
                     NLDEPPLPQTGEVARIHRYATDFEALRDRSDAFLQRTGARPRVLLLPVGPLAEHNIRA
                     TFAANLLASGGIEAINPGPLDEAGIDAAVQEAGRPNIAIICGTDARYGSEVAGVVRAA
                     HAAGITAIYLAGPRKAVADTDQSQLPDEYLTASIEAVHALSNLLGRLGA"
     gene            2455..>4464
                     /gene="scpA"
                     /locus_tag="VF874_10920"
     CDS             2455..>4464
                     /gene="scpA"
                     /locus_tag="VF874_10920"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX9176559.1"
                     /translation="MSTPTSVVGSFADVPLHGEQVPAPPAEADVTALVAAAATAHGYT
                     ADQLDWHTPEGIVVKPVYTAADRAATAVEGYPLNNFPGEPPYLRGPYPTMYVNQPWTI
                     RQYAGFSTAADSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVQGDVGMAGVAID
                     SILDMRQLFDGIDLSTVSVSMTMNGAVLPILALYVVAAEEQGVPPEKLAGTIQNDILK
                     EFMVRNTYIYPPKPSMRIISDIFAYTSAKMPKFNSISISGYHIQEAGATADLELAFTL
                     ADGVEYIKAGLEAGLRIDQFAPRLSFFWGIGMNFFMEVAKLRAGRLLWSELVSQFNPD
                     NPKSLSLRTHSQTSGWSLTAQDPFNNVARTCIEAMAATQGHTQSLHTNALDEALALPT
                     DFSARIARNTQLLLQQESGTTRPVDPWGGSYFVEWLTHQLADQARAHIREVEEHGGMA
                     QAISDGIPKLRIEEAAARTQARIDSGQQPVVGVNKYQVDEDTTIEVLKVENSRVRAEQ
                     LAKLQQLRADRDQSKVKAALQELSRAAAGDDNLLELSIDAARAKATVGEISDALEKVY
                     GRHAAEIRTISGVYRHEIGKGTNIASATELVEKFAEADGRRPRILVAKMGQDGHDRGQ
                     KVIATAFADIGFDVDVGSLFSTPEEVARQAADNDVHVIGVSSLAAG"
BASE COUNT          792 a         1541 c         1475 g          656 t
ORIGIN      
        1 cccgacgaac gtcgccaccg tgtacggcga cactcgcacg gcggatgccc cggcggcgta
       61 attgatcacc gagaacggca ccgccggaat cagtcgcagc gacaagacaa tcgcccagcc
      121 gcgctgccgg aaccgcgcgt caacggcctc gatgccggga tggcggacca gccggtcgag
      181 ctgccagccg gcaacgcgca ccagccacaa cgcgatcagc gcgctcagcg tgctggcggc
      241 caccgccaac gtgacacccc gtgctgtgcc gaacaacaac ccggcagcca acgtgaacgc
      301 agtacgggga aacgggaaca ccgtcaccac cacgtgcgcg gccaggaacg ccagcgggaa
      361 ccacccaccg gccgacgtcg cccagtcgcg cagctcaacc gccgtcggca tcggcacgaa
      421 gacgacggct gcgaccaaga tcacaacgcc gatcagcgtc gcgacgatgc gccgccgcga
      481 gacctgccgc gcggacacga ccgtcgcaaa ccgcagtccg cgcagcgtgt ggcggatttt
      541 ggcgagggtc atgcctgttt accctacgac gttggacggg tgggcatcat ttagcctgat
      601 ggcgtgtcaa ctcaagcccg tgtcgactcc gctgaggtag agggggcgct gagccagtgg
      661 cgctcgcagg tggccgctgt cctggccaaa cgcagcaccg atctccccgc cgacccggag
      721 cgtatgctcg acacgccgac ctacgaaggc ttcccggtcc gcgcgctgta caccgcgctg
      781 gacgcccgcc cggaagatcc gttgccgggc cgatggccgt tcgttcgcgg cggtgacgcg
      841 ctgcgcgacg tcaacaccgg ctggaaggtc gccgaggcgt ttcctgcgca cgggggcgcg
      901 gccgtcggtg acctcaacgg cgcggtgctg ctggcgttga ctgaaggcgt cagcgcgctg
      961 gtgctccggg tcggggacgc ccgggtgccg cccgacgccc tggaccggct gctcgagggc
     1021 gtgttcatcg agctcgtgcc gatcgtgctt gacgcgggtg ccgacctcgg cgcggccgcc
     1081 gacgcggtgc tggcattggc agccaccctc gacgaggaca agtacgcgat gctgtcgatc
     1141 gacctgggcg ccgacccgct gaccgcggcg ttgagcgggc gggcggcgcc gggcctcgac
     1201 gaggtcgtcg cattcgcgtc gcgggcagcc gcctatcgtg gcggagtccg ggcaatcacc
     1261 gtggacggac cggtatttca caaccgcggt gcgaacgcgt cgtgggagct ggcaggcagt
     1321 gtcgcggctg gggtggccta tctgcgctcg cttccggact tgagcgtcgc gcaggcgctt
     1381 cgccagatca gcttccgctt cgccgctgac gacgaccagt tcatgacgat cgcgaaattc
     1441 cgtgctgcac gccaactttg ggcgcgagtg gccgaggtcg ccgaagcgcc cggcgccggg
     1501 gccgcgacca tgcacgccgt cacgtcgctg ccgatgatga cgcagcgcga tccgtgggtg
     1561 aacatgctgc gcaccacggt cgcggcgttc gcagccggaa tcggcggtgc cgaaacggtt
     1621 ttggtggaac cgttcgacgc agcaatcccg ggtggcgcgc ccggggtgag cgccggcttc
     1681 tcgcgccgca tcgcccgcaa cacccagctg ctgttgctcg aggagtccaa catcgggcgg
     1741 gtgctggacc cggcgggcgg gtcgtggtac gtcgaagacc tcaccgaagc gcttgcccag
     1801 caggcgtgga gccacttcca ggcgatcgag tcccgaggtg gattcaccga cgcgcacgac
     1861 ttcatcgccg cgcatctcca acaggtgagt gaccagcgca gcgacgacgt cgaccaccgg
     1921 cgcacgaaga tcaccggcgt caacgagtac ccgaacctcg acgagccacc gctgccgcag
     1981 accggtgagg ttgcgcgcat ccaccggtac gcgacggact tcgaagcgct gcgcgaccgc
     2041 tcggatgcgt tcttgcagcg cacaggtgcg cggccacggg tgctgctgtt gccggtgggc
     2101 ccgcttgccg agcacaacat ccgggccacg ttcgcggcga acctgttggc gtccggcggt
     2161 attgaggcca tcaaccccgg accgctcgac gaggcgggca tcgacgcagc ggtccaggaa
     2221 gccggccggc cgaacattgc gatcatctgc ggaaccgatg cgcggtacgg ctccgaagtc
     2281 gcgggtgtgg tgcgggccgc acatgccgcg ggtatcacgg cgatctatct tgcgggccca
     2341 cggaaggcgg tcgcggacac agaccaatcg cagctgcctg acgagtatct gaccgcgagc
     2401 atcgaggccg tccatgcgct gtcgaacctg ctcggccgat tgggggcgtg aactatgagc
     2461 acacctacct ccgtcgtcgg cagtttcgcc gacgtccccc tgcacggtga acaggtgccc
     2521 gccccgcccg ccgaagccga cgtgaccgca ctcgtcgccg cggcggccac ggcgcacggc
     2581 tacaccgccg accaattgga ctggcacaca ccggaaggca tcgtcgtcaa accggtctac
     2641 accgccgccg accgcgccgc gacggctgtg gagggctatc cgctcaacaa cttccccggc
     2701 gaacctccct acttacgcgg gccgtatccg acgatgtacg tcaaccagcc ctggacgatt
     2761 cgtcagtacg cgggattttc caccgcggcc gactcgaacg cgttttaccg acgcaacctg
     2821 gcggccggac agaagggcct ctcggtcgcg ttcgacctgg ccacccaccg cgggtatgac
     2881 tccgaccacc cgcgtgtgca gggtgacgtc ggaatggccg gagtggcaat cgattccatt
     2941 ctcgacatgc ggcagctgtt cgacggcatc gacctgtcga cggtgtcggt gtcgatgacc
     3001 atgaacggcg cggtgcttcc gattctcgcg ctctacgtgg tggcggccga ggaacagggc
     3061 gtgccaccgg agaagctggc cgggaccatc cagaacgaca tcctcaaaga gttcatggtc
     3121 cgtaacacct acatctaccc gccgaagccg tcgatgcgga tcatctccga catcttcgcc
     3181 tacaccagcg cgaagatgcc gaagttcaac tcgatctcga tctccgggta tcacatccaa
     3241 gaggccggtg cgacagcgga tctcgaactc gccttcaccc tcgccgatgg cgtggaatac
     3301 atcaaggccg gactggaagc gggcctgcgc atcgaccagt tcgcgccgcg gttgtcgttc
     3361 ttctggggca ttgggatgaa cttcttcatg gaggtcgcca agctgcgtgc cggccggctc
     3421 ctgtggagcg agctggtttc ccagttcaac cccgacaacc cgaaatcact gtcgttgcgc
     3481 acgcattcgc agacctcagg gtggtcgctg accgcgcagg acccgttcaa caacgtcgca
     3541 cgcacctgca tcgaggcgat ggccgccacc cagggccaca cccagtccct gcacaccaac
     3601 gcactcgacg aggcgctggc gctgccgacc gacttctccg cccgcatcgc ccgcaacaca
     3661 cagctgctgc tgcagcagga gtccggcacg acccggcccg tcgacccatg gggcggttcg
     3721 tatttcgtcg aatggctcac ccaccaactc gccgatcagg cccgcgcgca catccgcgag
     3781 gtcgaagagc acggtggcat ggcgcaggcg atcagcgacg gcatcccgaa gctgcgcatc
     3841 gaggaggccg ccgcccgcac gcaagcccgc atcgactccg gtcagcagcc ggtggtcggc
     3901 gtgaacaaat accaagtcga cgaggacacg acgatcgagg tgctcaaggt cgagaacagc
     3961 agggtgcgcg ccgagcaact cgccaagttg cagcagctgc gcgccgatcg agatcagtcc
     4021 aaggtcaagg cggcgcttca ggagctgagc cgggccgccg ccggggacga caacctgctg
     4081 gagttgtcga tcgacgccgc acgcgccaag gccacggtgg gggagatctc cgacgccctc
     4141 gagaaggtgt acgggcggca cgcggccgaa atccgtacca tttccggggt ctaccgccac
     4201 gagatcggaa aggggaccaa catagcgtcc gcaaccgagc tcgtcgaaaa gttcgcagag
     4261 gccgacggcc gccggccgcg catcctggtg gccaagatgg gtcaggacgg ccacgaccgc
     4321 ggtcagaagg tgatcgcaac cgcgttcgcg gacatcgggt tcgacgtcga cgtcggatcg
     4381 ctgttctcca cgcccgagga ggtggcgcgc caggccgccg acaacgacgt gcacgtgatc
     4441 ggggtgtcct cgcttgccgc cgga
//
LOCUS       DASWBF010000304         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_288143,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000304 DASWBF010000000
VERSION     DASWBF010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288143"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..755)
                     /locus_tag="VF874_10925"
     CDS             complement(132..755)
                     /locus_tag="VF874_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9176560.1"
                     /translation="MPGADSVTTAILDAAVAEFEQHGFRRVALDDVARRAGVSRMTIY
                     RRFANRDELVTAVIDRENAVLFTEIADELKDAGPQSNYYVEAFTSAIMQLRRHRVLNR
                     MITDEPALTLQLAHRHYPAAIGRMADALRVIFPAGFAERIGEQAVDELADTILRYALM
                     ALLLPSSRPLDTADDVRVFATAHFLPSLPSALRERELATSSKEDRLT"
     gene            complement(742..1595)
                     /locus_tag="VF874_10930"
                     /pseudo
     CDS             complement(742..1595)
                     /locus_tag="VF874_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
     gene            complement(1648..>1828)
                     /locus_tag="VF874_10935"
     CDS             complement(1648..>1828)
                     /locus_tag="VF874_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEX9176561.1"
                     /translation="KQLPLAYGHFGFGGSGAFADPEHDLALAMVCNRGSGTPIGDQRI
                     LRLSQAAARAVRTAG"
BASE COUNT          308 a          608 c          613 g          299 t
ORIGIN      
        1 ctttcgacac cccgcttagc ccgccgcgtc tccacgacga accggggcct gctacccggc
       61 gttccggtac ctaccgggac gggacttcca cccgctagcc tgatccagct ttctggacgc
      121 accatgcggg cctacgttag acgatcttct ttggatgatg tcgccagttc acgttcgcgc
      181 agcgccgagg gcaggctcgg cagaaagtgg gcggtggcga acacccggac gtcgtctgca
      241 gtgtccagcg gtcgcgatga cggcagcagc agcgccatca acgcgtagcg caggatcgtg
      301 tcggcgagtt cgtcgacggc ctgctcaccg atccgctcgg cgaagccggc cgggaaaatc
      361 acccgcagcg cgtcggccat ccgcccgatc gccgcggggt aatgccgatg cgcgagctgc
      421 agtgtcagtg ccggctcgtc ggtgatcatc cggttcagca cgcgatggcg gcgcagttgc
      481 atgatcgccg acgtgaacgc ctcgacgtag taattactct gcggcccagc gtctttcaac
      541 tcatcagcaa tctcggtgaa cagcacggca ttctcgcggt cgatcacggc ggtgacgagc
      601 tcgtcgcggt tggcgaaccg gcggtagatc gtcatccggc tcaccccggc gcggcgggcg
      661 acgtcgtcga gcgccacccg gcgaaacccg tgctgctcga actcggcaac ggccgcgtcc
      721 agtatcgcgg tggtgacgga gtcagctccg ggcatagccg gcctgcgcaa acttgttgta
      781 gcgcaaccgc atcggcagcc ggtcccatat ccagttgacc ggccgggacc ggcacatcgc
      841 tgcgaaccgc tgatatcgcc gctcctgtcg atcgctccac ggcagcctga gaagcgtgcg
      901 cgcgcgtggc ggtagcgcgc cggtggtcag gaaggcggcc agcgggttga acaccagcgc
      961 gacggcccgc cacagcgcag gcggcacacc cccggggcac gggaagccct tggtgacgta
     1021 gccgacgccg tacttcgcgg ttgggtgggc gacgacgacc ccgcggagca tgcggtcccc
     1081 gtaccgctcg aaggcggcgt agtcggcggg catcgcccgc tcgctgacgc cgtaacggcg
     1141 gtaccacgtc ttggactccg agtagatctg ctccttctcg gcgcggctga gccgcttgac
     1201 aacgtatcgc cgaaatacag cacctgctcg acgaacgtcg cgtgcgccca gaagtacgtc
     1261 tgcgggtcga gcgcgtgata gcggctgccg ccgggcatct cccccttgat ctggcgatgg
     1321 aaatcgcgga cctgctgccc cgggtgctcg gtgtcggagc cgtagacggt gttgaagatc
     1381 ggcggcagtg accgcttcat gcgggcggcg gtgtcgtcga aaaacacgga gtggtccagc
     1441 acgccctggc ccagttcggc gagcatgttc tgcagcaccg ccggccgcgg gccgatcagg
     1501 aacatccggt tgtcgccgaa atatctccag actagagact ggggccccaa cggctgggcg
     1561 tctgcgtctg gcatcgccag cgctagctcg gtcatggaaa cagtgttaca aatctcgcac
     1621 tctgtaccaa gacccttgtg acgaggttca ccccgccgtg cggacggcgc gggccgcggc
     1681 ctgactgagg cgcagaatgc gctggtcccc gatcggggtg ccactgcccc ggttgcacac
     1741 catcgccagg gccaggtcgt gctcgggatc ggcgaaggcg ccggagccgc cgaaaccgaa
     1801 atggccatag gcaagaggga gttgcttg
//
LOCUS       DASWBF010000305         5764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_234091,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000305 DASWBF010000000
VERSION     DASWBF010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234091"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VF874_10940"
     CDS             <1..281
                     /locus_tag="VF874_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9176562.1"
                     /translation="SKFDAEKVFTACRDHGTAVEINSRPERRDPPRRLMDLALQIGCV
                     FSIDTDAHAPGQLEFLAYGAQRALDASVPAERIVNTWPADQLLAWSRG"
     gene            complement(254..790)
                     /locus_tag="VF874_10945"
     CDS             complement(254..790)
                     /locus_tag="VF874_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9176563.1"
                     /translation="MRVTRKQAELNRQRIIESAIRLFAERGVDGVGVAEVMADAGFTH
                     GGFYNHFPSKDDLVNEACKTSFALAVSSLREGNRPLDRLRDDDFGACLSAAMLSEASK
                     GNNARLGTVYNKGVGDLLDVLTEQHGDPDRAAAALSCFVGARVLARAVQRADRNLAEQ
                     FDTVSYPQGAQPRLQASS"
     gene            complement(863..2062)
                     /gene="dinB"
                     /locus_tag="VF874_10950"
     CDS             complement(863..2062)
                     /gene="dinB"
                     /locus_tag="VF874_10950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9176564.1"
                     /translation="MFVPGGATILHADLDSFYASVEQRDDPALRGRPVIVGAGVVLAA
                     SYEAKAYGVRTAMSGGQARRLCPHAVVVPPRMSAYSAASDAVFDVFRDTTPLVEPLSV
                     DEAFLDVGGLRRIAGSPVAIAAKLRADVRERVGLPITVGIARTKFLAKVASQVAKPDG
                     LLLVPPDRELAFLQPLPVRRLWGVGAVTADKLHAHGIETVADVVELGQPMLASLVGWA
                     MGRRLHALSRNVDARRVQTGVRRRSVGAQRALGRGHRTAPELDVILITLIDRITRRMR
                     AAGRTGRTVVLRMRFDDFSRATRSHTLPRATASTEPLVAAARELVSAATPLIAQRGLT
                     MIGFSVANIDRGGTQQLELPFESGPDAFAIDTAVDRVRQRYGTAAVTRAVLLNGDVGL
                     EMPHLPD"
     gene            2126..3604
                     /locus_tag="VF874_10955"
     CDS             2126..3604
                     /locus_tag="VF874_10955"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9176565.1"
                     /translation="MSEHLDVLIVGAGISGISAAWHLQDRCPSKRYAILESRADLGGT
                     WDLFKYPGIRSDSDMYTLGFRFKPWTSERGIADGPSILTYLKETAAEFGIDKHIRYHH
                     KVLAADWSDAENKWFLRIDRDGEETEITCSFLFACSGYYNYDEGYSPEFPGSNDFQGI
                     IVHPQHWPQDLDYQGKKIVVIGSGATAVTLIPALANSGSGHVTMLQRSPTYIGSLPSV
                     DPFAVRMQKRLPPKLAYLAIRWKSLLYQAAIFRLSRKRPDFMRKQLMTMAKRRLPEGY
                     DVEKHFGPSYNPWDQRLCLAPDGDLFRAIRHGKADVVTDTIDRFTMTGVRLSSGEQLD
                     ADIIITATGLNMQVFGGAAVLRNGVPVEFNKHMAYKGMMLSGLPNVAMTLGYTNASWT
                     LKADLVSEYVCRLLNYMDDNGFDTVEPQHPGASVEERPPMDLTSGYVLRALDRLPKAG
                     AKAPWRFPHNYLVDIRAIRHGKIDDPALRFSRHRAAVSAASA"
     gene            complement(3585..4106)
                     /gene="rraA"
                     /locus_tag="VF874_10960"
     CDS             complement(3585..4106)
                     /gene="rraA"
                     /locus_tag="VF874_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HEX9176566.1"
                     /translation="MNSPAEPRATADLVDDIGADVRSCDTQFRQFGGRSMFAGEIHTV
                     RCFEDNALLKSVLSEPGDGGVLVIDGSASLHSALVGDLIAGLGQSNGWAGIIVNGAVR
                     DAAALRQIDIGIKALGTNPRKSSKSGAGERDIQVEFGGITFVPGELVYSDDDGIVVVA
                     ERTTSGGVTPTPR"
     gene            complement(4241..4738)
                     /locus_tag="VF874_10965"
     CDS             complement(4241..4738)
                     /locus_tag="VF874_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176567.1"
                     /translation="MADEQDRPDQPSQTPPPAAEPPASEPPPAKHTGAKAAKKAPAKA
                     AKKAPAKAAKAAKGAKAAPKKAAKKAPPPPPKPEPTPRPPIAAEPQAAATLADTNGDT
                     RFGAADETAAQARQAVDTAGDSLGSGISRVPGTGGRVSIAVALAMALLAILLIRRLLS
                     DDQTA"
     gene            complement(4844..5344)
                     /locus_tag="VF874_10970"
     CDS             complement(4844..5344)
                     /locus_tag="VF874_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEX9176568.1"
                     /translation="MRLVALLGGLLVAGAVAAAPVASAHTARIASDPVEAAVLTNGPA
                     HVSATFSEPLQAQFASMTVVGPDGNLWSTGEPRVVGAVISIDVRPLGPPGRYTVNYRV
                     TSADGHPVTGSWWFELKAAGTGTPGPPAGAPASTGGVPVWPFVAAGAALVAIAAVWAL
                     RRVNRS"
     gene            complement(5551..>5764)
                     /locus_tag="VF874_10975"
     CDS             complement(5551..>5764)
                     /locus_tag="VF874_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6474 family protein"
                     /protein_id="HEX9176569.1"
                     /translation="QSVRLVAEKNPKDAETKRFVAAITGRLKELSAAVTAAENMPSAR
                     RRAAHSAIATDLDGIDADLMARLGLH"
BASE COUNT          910 a         1995 c         1923 g          936 t
ORIGIN      
        1 aatccaagtt cgacgcggag aaggtgttca ccgcctgccg agaccacggc accgccgttg
       61 agatcaactc ccgacccgaa cgccgagacc cgccgcgacg gctgatggat ctggcactac
      121 agatcggttg cgtgttctcg atcgacaccg acgcgcacgc cccgggtcaa ctcgagttcc
      181 tggcctatgg cgctcagcgt gcgctggacg ccagcgtgcc ggcggagcgc atcgtcaaca
      241 cctggcccgc cgatcagctg ctggcctgga gccgcggctg agcgccctgc gggtacgaca
      301 cggtgtcgaa ctgctcagcc agattgcggt cggctcgttg caccgcgcgt gcgagcactc
      361 gggcgccgac gaagcacgac aatgcggccg cagcgcgatc gggatcgccg tgttgctcgg
      421 tgagcacgtc gaggaggtca ccgaccccct tgttgtatac ggtgcccagc cgcgcgttgt
      481 tgcccttcga cgcttcactc aacatcgcgg ccgacaggca cgcgccgaag tcgtcgtcac
      541 gcaaccggtc cagcggccga tttccttcgc gcagcgacga caccgccaac gcgaacgacg
      601 ttttgcacgc ctcgttcacg agatcatcct tcgacgggaa atggttgtag aaacccccgt
      661 gggtgaaccc cgcatcggcc atcacctcgg cgacgccgac gccgtcgact ccgcgttctg
      721 cgaataagcg gattgcgctc tcgatgatgc gttgtcgatt cagttcggcc tgtttcctgg
      781 tgacccgcat gtgccagttc ctcccgtatc cctgatagtc cggttacccg gcacacggtg
      841 gagtcatgcg tgttggccgt ggtcaatccg gcagatgagg catttccagc ccaacatcgc
      901 cgttcagcag caccgcccgg gtgaccgcag cggtgccgta gcgctgacgc acgcggtcga
      961 ctgcggtgtc gatcgcgaag gcgtcgggcc ccgactcgaa gggcagctcc agctgctggg
     1021 tgccgcctcg gtcgatgttg gcgacgctga acccgatcat cgtcaagccg cgctgggcga
     1081 tcagcggcgt tgcggcggag accaattcac gggctgcggc cacgagcggt tcagtggacg
     1141 cggtggcccg cggcagcgtg tgcgagcggg tggcacggct gaagtcgtcg aagcgcatcc
     1201 gcagcacgac cgttcggccg gtgcggccgg cggcgcgcat ccggcgggtg atccggtcga
     1261 tgagcgtgat caaaatcacg tccaactcgg gggcagtgcg gtggccgcgt ccgagcgcgc
     1321 gttgagcccc gaccgatctg cgccgcactc cggtctgcac acgccgagcg tcgacatttc
     1381 gcgacagcgc gtggagccgg cgccccatcg cccagccgac cagagatgcc agcatgggct
     1441 ggccgagttc gaccacgtcg gcgacggttt cgatgccgtg ggcgtgcaac ttgtcggccg
     1501 tcaccgcgcc gacgccccac agccggcgca ccggcagcgg ctgcaggaac gccagctcac
     1561 ggtccggcgg gacgaggagc aggccgtcgg gcttggcgac ctggctggcg accttggcga
     1621 ggaacttcgt gcgcgcgatc ccgacggtta tcggtaggcc cacccgctcg cggacgtcgg
     1681 cgcgcaactt cgcggcgatg gccaccgggc tgcccgcaat ccggcgcagt ccgccgacgt
     1741 cgaggaacgc ctcgtccacg gacagcggtt cgacgagcgg cgtggtgtcg cgaaacacgt
     1801 cgaacaccgc gtcggacgcc gcgctgtagg cggacatgcg cggcggcacg acgaccgcgt
     1861 gcggacacag tcgccgggcc tggccgccgc tcatcgcggt ccgcaccccg tacgccttcg
     1921 cctcgtagct ggcggcgagc accacgccgg cgcccacgat caccggccgt ccgcgcagtg
     1981 cgggatcgtc ccgctgctcg accgacgcgt agaacgagtc gagatcggcg tgcaggatcg
     2041 tcgctccacc aggcacgaac atatgttcgc atccgtcggg gacacatcga agagtgtcct
     2101 cccaataggc ggtaccgttg gtcccatgtc cgaacatctc gatgttctga tcgtcggcgc
     2161 cgggatctcc ggcatcagcg ccgcctggca tctgcaggac cgctgcccgt cgaagaggta
     2221 cgcgatcctg gagtcgcgcg ccgatctcgg tggcacttgg gacctgttca agtacccggg
     2281 catccgctcg gactccgaca tgtacaccct gggcttccgg ttcaaaccgt ggacgtcgga
     2341 gcgtgggatc gccgacggcc cgtcgatcct gacctacctg aaggagacgg ccgccgagtt
     2401 cggcatcgac aagcacatcc gctaccacca caaggtcttg gccgccgact ggtccgacgc
     2461 cgagaacaaa tggttccttc gcatcgatcg cgacggcgag gaaaccgaga tcacctgctc
     2521 gtttctgttc gcgtgcagcg gctactacaa ctacgacgag ggctactcgc cggagttccc
     2581 cggttccaac gactttcaag gcatcatcgt tcacccgcag cactggccgc aggacctcga
     2641 ctaccaaggc aagaagatcg tcgtgatcgg cagcggcgcc actgccgtca ctttgattcc
     2701 cgcgcttgcc aattcaggat ccgggcacgt gacgatgctg cagcgctcac cgacctacat
     2761 cggctcgcta cccagcgtcg acccgttcgc cgtccggatg cagaaacggc tgccaccgaa
     2821 gctcgcgtac ctcgcaatcc ggtggaagag cctgctgtac caggccgcga tatttcgcct
     2881 cagccgcaag cgcccggact tcatgcgcaa gcaactgatg acaatggcca agcggcgctt
     2941 gcccgaaggc tacgacgtcg aaaagcactt cggccccagc tacaacccgt gggatcagcg
     3001 cctgtgcctc gcccctgacg gcgacctgtt ccgcgccatt cgacacggca aggctgacgt
     3061 cgtcaccgac acgatcgacc ggttcaccat gactggggtc aggctgtcct ccggcgaaca
     3121 gctcgacgcc gacatcatca tcaccgcaac gggtttgaac atgcaggtgt tcggcggagc
     3181 ggcggtcttg cgcaacggcg tgccggtgga gttcaacaag cacatggcat acaagggcat
     3241 gatgctgagc gggctcccga acgtggcgat gacgctcggc tacaccaacg cgtcgtggac
     3301 tctgaaggcc gacttggtct ccgagtatgt ctgccggctg ttgaactaca tggacgacaa
     3361 cggatttgac accgtcgagc ctcaacaccc cggtgccagt gtggaggagc gcccgccgat
     3421 ggacttgacg tcgggctatg ttctgcgggc gctcgaccgg ctgccgaaag ccggtgcgaa
     3481 ggcgccctgg cggttcccgc acaactacct ggtggatatt cgcgcgatcc ggcacggcaa
     3541 gatcgacgat ccggcactgc gattctcccg tcaccgggca gccgtcagcg cggcgtcggc
     3601 gtgacgccgc ctgatgtcgt ccgctccgcg acgacgacga tcccgtcatc gtcgctgtag
     3661 acgagttcgc ctggcacaaa agtaattccg ccgaactcga cttggatgtc gcgttccccg
     3721 gcgccggact tgctgctctt gcgtgggttg gtgcccagcg ctttgatgcc gatgtcgatc
     3781 tggcgcagcg cggcggcgtc acggaccgcg ccgttgacga tgatcccggc ccacccgttg
     3841 gactggccta ggccggcgat caggtcgccg acgagcgcgc tgtgcagcga cgctgatccg
     3901 tcgatcacca gcacgccacc gtcacccggc tcggacagca cagatttcag cagtgcgttg
     3961 tcttcgaagc accgcacggt gtggatctcg cccgcgaaca tcgagcgccc gccgaactgg
     4021 cggaactggg tgtcgcagct gcggacgtcg gcaccgatgt cgtcgacgag gtctgcggtg
     4081 gcgcgaggtt ctgccgggct attcacgctc cagaatctac gtggaggtgc gaggtgacca
     4141 cgatgtcgtg cacgagctgc gccaatcccg gctcggcgtc ctcgtagtac ggagtgaacc
     4201 ggtgcatgag cccactatcg gccgtgacgc cgcggcaggg tcaagcggtc tgatcgtccg
     4261 agagcagccg ccggatcagc aggatggcca gcagcgccat cgccagggcc accgcgatcg
     4321 acacccgtcc gccggtgccg ggcacccgag agatcccgct gcccaggctg tccccggcgg
     4381 tgtcgaccgc ttggcgcgcc tgcgccgcgg tctcatcggc cgcaccgaag cgcgtatcgc
     4441 cgttggtgtc ggcgagtgtt gccgccgcct gcggttcggc cgcgatgggt ggccgaggcg
     4501 tcggctcagg cttcggcggt ggcggcggcg ccttcttggc ggccttcttc ggtgccgcct
     4561 tcgcgccctt ggcggctttg gcggccttcg cgggcgcctt cttcgccgcc tttgcggggg
     4621 ccttcttggc ggccttggct ccggtgtgct tggcgggagg cggctccgac gcgggtggct
     4681 cggcagcggg cggcggggtt tggctcggct ggtcgggtcg atcctgctcg tctgccatgt
     4741 ggcggctcct tcgcagtttc tcgtagggta cccatcatgc cagcggccac ccgaggcccc
     4801 ctatccggtc accgccaacg ctgcggccat cgccgtcctc atctcagctg cggttgaccc
     4861 ggcgcagggc ccacaccgcg gcaatcgcga cgagcgctgc gcctgcggcc acgaacggcc
     4921 agaccggtac gccgcccgtc gacgcgggcg cccccgccgg ggggcccggc gttcccgtgc
     4981 cggcggcctt caactcgaac caccatgaac ccgtcacggg gtgcccgtca gccgaggtca
     5041 cccggtagtt gacggtgtag cggccgggtg gcccgagcgg gcggacgtcg atgctgatca
     5101 ccgcgccgac aacgcgcggc tcgccggtgg accacaggtt gccgtccggc ccgacgactg
     5161 tcatcgaggc gaactgcgcc tgcagcggct cgctgaacgt tgcgctgaca tgagccgggc
     5221 cgttggtcag caccgccgcc tcgacgggat cgctggcgat gcgcgcggtg tgcgcggaag
     5281 ccaccggcgc cgcggccaca gccccggcca ccagcagccc gccgagcagg gcgacgagcc
     5341 tcatgaccgt cgggcgtacc aacgccagcg tgaccgcggc cgcgccgacg gcgaggaccg
     5401 cggcgggtat cagggcgcgc tgaagcgccc ccgcacccga tggggcggct ccgtcggcga
     5461 gcccatgtgc acccacggac aacgccacgg tgagcagcgc cgccgatgca ccgcgccaac
     5521 gcgcagcgga gtcgaggcgg cgcctgcgga tcagtgcagc cccagtcggg ccatcagatc
     5581 cgcgtcgatg ccgtccagat ctgtggcgat tgctgagtgc gcggcgcggc ggcgagcgga
     5641 gggcatgttc tcggccgcgg tgacggcggc ggacagctct ttcagccggc ccgtgatcgc
     5701 ggcgacgaac cgcttggttt cggcgtcttt gggattcttc tcggcgacga gacgcaccga
     5761 ctgt
//
LOCUS       DASWBF010000306        24811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_811047,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000306 DASWBF010000000
VERSION     DASWBF010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24811
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811047"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            516..656
                     /locus_tag="VF874_10980"
     CDS             516..656
                     /locus_tag="VF874_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176570.1"
                     /translation="MGAKTAVSVEVGTPAYIEVFGKVADTLAARRRGLAGMPLGIDTA
                     RR"
     gene            complement(762..1058)
                     /gene="tatB"
                     /locus_tag="VF874_10985"
                     /pseudo
     CDS             complement(762..1058)
                     /gene="tatB"
                     /locus_tag="VF874_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
     gene            complement(1058..2488)
                     /locus_tag="VF874_10990"
     CDS             complement(1058..2488)
                     /locus_tag="VF874_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9176571.1"
                     /translation="MSRPPVDPASSRAFGRPAGFDGSFLGADKHRDQGEFTPRNAPPD
                     PVLAEAFGRPYSGGESLQRHPSDVGALDGERDDDADLAADPWRDPNAAASLTTPALPK
                     PQPARASATTGKLGVRDVLFGGKVSYRALAVLAVIALALAFVGGWVGRKTAEVVEAFT
                     TSKVTLSTSGGQAPEGRFAQVAAAVADSVVTVEALSDQEGSQGSGVVVDGRGYIVTNN
                     HVISEAANNPSQFKITVVFNDGKEVPANLVGRDPKTDLAVLKVDNVNNLTVAQFGDSD
                     KVHVGDEVIAAGAPLGLRSTVTSGIISALHRPVPLSGEGSDTDTVIDALQTDAAINHG
                     NSGGPLIDMNSHIIGINTAGKSLSDSASGLGFAIPINEVKAVVEALIKDGKIVHPTLG
                     LTARSVSNSVASGAQVANVKAGGPAEKGGVLENDVVVKVGDRTVADADEFVVAVRALT
                     IGQPAPIEVVRDGRHVTLTVTPAPDN"
     gene            complement(2681..3112)
                     /gene="rseA"
                     /locus_tag="VF874_10995"
     CDS             complement(2681..3112)
                     /gene="rseA"
                     /locus_tag="VF874_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma E factor RseA"
                     /protein_id="HEX9176572.1"
                     /translation="MADRGHLFRRAFSWLPSQFASQSDAPVGAPREFGSTEHLSTEAI
                     AAFVDGELRMTAYLRAAHHLSLCPQCSAEVEGQRQARAALRDSRPIDVPTSLLGTLAQ
                     IPHQSDDVDEPLGRRASKPVDEPGAAAPDDLGVPRGRRKRR"
     gene            complement(3215..4066)
                     /gene="sigE"
                     /locus_tag="VF874_11000"
     CDS             complement(3215..4066)
                     /gene="sigE"
                     /locus_tag="VF874_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
                     /protein_id="HEX9176573.1"
                     /translation="MVHAGWRVGNSPDEIDVSRFEEVLPLSTGGEVYPLDREDPTTRT
                     LSPTPMPPATMSHFERSFDSGWVEETDEPQGTAVFDATGDKSTMPSWDELVRQHADRV
                     YRLAYRLSGNQHDAEDLTQETFIRVFRSLQNYQPGTFEGWLHRITTNLFLDMVRRRAR
                     IRMESLPDDYDRVPADEPNPEQIYHDSRLGPDLQAALDSLPPEFRAAVVLCDIEGLSY
                     EEIGATLGVKLGTVRSRIHRGRQALRDYLAAHGSSEELVAKSSALRASGSSLPSALRA
                     SGPSLSA"
     gene            4217..4849
                     /locus_tag="VF874_11005"
     CDS             4217..4849
                     /locus_tag="VF874_11005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX9176574.1"
                     /translation="MTGTKLGILGHAEDSISEDPIVAAARERAVDAGAGAVTPAVGAL
                     LSVLARLSGAKAVAEVGTGAGVSGLWLLSGMADDGVLTTIDVEPEHQRLAKQAFTEAG
                     IAPSRTRVIAGRAQDVLQRLADESYDLVFVDADPVDQPHFVIEGVRLLRPGGAIVIHR
                     AALGGRAGDPSAADGEVTAVREAARLIAEDERLTPVLVPLGDGVLVAARD"
     gene            complement(4858..5094)
                     /locus_tag="VF874_11010"
     CDS             complement(4858..5094)
                     /locus_tag="VF874_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176575.1"
                     /translation="MSTPLQRSRLTQRDSAHAGSAPALLRMLNLHDASVDKQAAALRV
                     WLTDNPPGPSLRLSLLANGYGHILDEAVGRRHSA"
     gene            complement(5233..6450)
                     /locus_tag="VF874_11015"
     CDS             complement(5233..6450)
                     /locus_tag="VF874_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176576.1"
                     /translation="MTAEFSLELPEEVVHVRDWVHEFAAEVIRPAASEWDEREQTPWP
                     IIAEAAKVGLYTPELFVQMAAEPSGIGMLSVFEETFWGDAGIALSILGTGLAAAALAA
                     SGTPEQLGEFLPQMFGSPGDPKVAAFCSSEPGAGSDVGAVRTRARYDEAADEWVLSGT
                     KTWATNGGIANVHVVVASVYPDLGTRGQATFVIPPDTPGLSQGQKFKKHGIRASHTAE
                     VVLDDVRIPGRLILGGRERFEQRVAKAREGKRTSSQAAMMTFERTRPAVGAMALGVAR
                     AAYEYALDYACQREQFGRKIGEFQAIAFKLADMKSRIDASRLLVWRAGWMHRNNKQFS
                     SAEGSMAKLFASETAVFVTDEAIQILGGNGYTRDYPVERMHRDAKIFTIFEGTSEIQR
                     LVMARALTGLPIR"
     gene            6664..7299
                     /locus_tag="VF874_11020"
     CDS             6664..7299
                     /locus_tag="VF874_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9176577.1"
                     /translation="MRSEDMTTAARIRDAAIEQWGQHGFHVGLRAIAEAAGVSASLVI
                     HHFGSKQGLRTACDDYIAEEVRSAKTESLRSADPATWFAQIAEIESYAPMMAYLVRSM
                     QSGTQLAKTFWQRMIDNAEEYLEEGVRAGTLKPSPDPKARAKFLAMSGGGAFLLYLQM
                     HENPTDMAAVLHDYGEEMVLPALEVYSHGLMTDSTMYDAFVAQREQVRSKA"
     gene            7296..8204
                     /locus_tag="VF874_11025"
     CDS             7296..8204
                     /locus_tag="VF874_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9176578.1"
                     /translation="MTDRQSDVPAVEIHGLRKSFGRTKALDGLDLTVERGSIAGFLGP
                     NGSGKSTTIRVLLGLLRAEAGTVRLLGGDPWHDAVSLHRRVAYVPGDVTLWPNLTGGQ
                     AIDFLARLRGGVDARRRDQLIERFELDPHKKARTYSKGNRQKVALVAAFSCDAELYIL
                     DEPTSGLDPLMERAFGNCVREVADRGAAVLLSSHILAEVEKLCDTVTIIRSGSTVRSG
                     GLAELRHLMRTTVKVRTRADTNGLHDAAFVHDFSSSDGVVTFSVNRRDLDQAMDRLTA
                     LGIDELSVYPASLEDMFLREYQGTNR"
     gene            8201..9799
                     /locus_tag="VF874_11030"
     CDS             8201..9799
                     /locus_tag="VF874_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEX9176579.1"
                     /translation="MTAGTHARPSAAPLTGWPNLLRLALRRDRIWLSVWISSLTLMML
                     YAPNAMKLAYPEEVQRFARVNLLKTPAGMMLGGPMFGGNETELGAMVANELMLTVIIA
                     VSIMAILTVIRHTRAEEESGAAELVMSSVVGRYARTFAALVLMGMVNGILAMTMTVAL
                     AATGLPVVDCAAMCLGITGVSMVFGAVAAVTAQLWRQARTASGAAMAVLAAVALIRGI
                     GDVIDNSGSALSWFSPIAWAQQMRPFVDLRWWPFVLLILLVVVLVAVAVALESRRQYD
                     DGVLPATGEHPNAHAIRGVFGLHETLQRGQVIGWSVALLLSGAAFGSMTKSLQDAAKS
                     NPLIARVLAAEGLDGIYTTMTQFLAAATTAFVAAAVLRLSRDEQSGLAEPVLAGSVSR
                     WRWLLTAVTAAVLSSAVLMLCAGFGNGLGAGITLGEPPTVARLTAAGLAFVPAMAVVA
                     AVAALAVAVKRPWIAWLFVTYIVLALYLGALLRLPRWLVDASPVGRTTAPTHVPVTAL
                     LMMIGVAIALTAVAGVIYRRRDVV"
     gene            9811..10485
                     /locus_tag="VF874_11035"
     CDS             9811..10485
                     /locus_tag="VF874_11035"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9176580.1"
                     /translation="MATALRMAGTSLLGLAAFGLLLFMPAGTLRYWQAWVFIAVFTIC
                     TLVPSTYLLVKDPAALQRRMHAGPAAETRTTQKIVIASAFLLVPAVMVSSAFDHRFGW
                     SPVPTAVSLIGQALVAIGLGITQYVIMQNSYAAATITVESGQKVVSSGLYSLVRHPMY
                     VGNLIMMVGIPLALGSYWGLLILVPGVVLLAVRILDEERALAQELAGYRDYMQKVHYR
                     LVPHIW"
     gene            10485..11069
                     /locus_tag="VF874_11040"
     CDS             10485..11069
                     /locus_tag="VF874_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX9176581.1"
                     /translation="MELLTGFGLATAAGLNAYIPILILGLLARFTDVITLPHAWSWLE
                     NGWVLTIVAVLLAVEVVADKIPALDSVNDAVQTVVRPTAGGIVFGSGTAAQTVAVTDP
                     GAFARSGQWIPIAIGIVVALVVHLTKTTVRPVANVATAGVAAPVLSTIEDITSVGLVV
                     VAIVLPVLVLVVVAALVWAAVKLLRRRRRARRRD"
     gene            complement(11129..12343)
                     /gene="glgC"
                     /locus_tag="VF874_11045"
     CDS             complement(11129..12343)
                     /gene="glgC"
                     /locus_tag="VF874_11045"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX9176582.1"
                     /translation="MRELPHVLGVVLAGGEGKRLYPLTADRAKPAVPFGGAYRLIDFV
                     LSNMVNARYLRICVLTQYKSHSLDRHISQNWRLSGLAGEYITPVPAQQRLGPRWYTGS
                     ADAIYQSMNLIYDEDPDYIVVFGADHVYRMDPEQMLTRHIESGAGATVAGIRVPRKEA
                     TAFGCIDSDESGRIRDFVEKPSDPPGTHDDPEQTFASMGNYIFTTKVLIDAVRADADD
                     DNSDHDMGGDIIPRLVDDGRAAVYDFDDNEVPGATERDRGYWRDVGTLDAFYDAHMDL
                     VSVHPIFNLYNKRWPIRGESENLAPAKFVNGGSAQESVVGAGSIVSAASVRNSVLSSN
                     VVVDEGAIVEGSVLMPGARVGRGAAVRHAILDKNVVVGPGEMVGVDLERDRERFAVSA
                     GGVVAVGKGIWI"
     gene            12464..13684
                     /gene="glgA"
                     /locus_tag="VF874_11050"
     CDS             12464..13684
                     /gene="glgA"
                     /locus_tag="VF874_11050"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX9176583.1"
                     /translation="MRVAMMTREYPPEVYGGAGVHVTQLVAQLRKLCEVDVHCMGAPR
                     HGAYVHQPDPALRGANPALTTLSADLVMANSVLAATVVHSHTWYTGLAGHLAALLYGI
                     PHVLTAHSLEPLRPWKAEQLGGGYRVSSWVERTAVETADAVIAVSSGMREDLLATYPA
                     LDPNVVHVVRNGIDTDVWCPVADARSKTGTADARSKKEEEPTLGESVLAELGVDVEKP
                     IVAFVGRITRQKGVAHLVAAAHRFAPEAQLVLCAGAPDTPEIAAEITEAVQNLSRARS
                     GVFWVREMLPIGKVREILSAATVFVCPSVYEPLGIVNLEAMACATAVVASDVGGIPEV
                     VADGDTGLLAHYDAGDPSSFETRLADAVNTLVADPDRAERFGRAGRQRCIDEFSWARI
                     AQQTLEIYRKVSAS"
     gene            complement(13681..13848)
                     /locus_tag="VF874_11055"
     CDS             complement(13681..13848)
                     /locus_tag="VF874_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HEX9176584.1"
                     /translation="MAAMKPRTGDGPLEATKEGRGIVMRVPLEGGGRLVVELTPEEAA
                     ALGDELKGVTS"
     gene            complement(13934..14509)
                     /locus_tag="VF874_11060"
     CDS             complement(13934..14509)
                     /locus_tag="VF874_11060"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX9176585.1"
                     /translation="MTDDGRVRCSWAQSGGAQAAMYRDYHDFEWGRPLHGRDALFERI
                     SLEAFQSGLSWLTILRKRENFRRAFDGFDVEKVARYTDRDVARLVADASIVRNRAKID
                     ATIANARAATGLDADLSELLWSFAPPPPRPRPATAADVAAVSPESIAMAKELKRRGFR
                     FVGPTTAYALMQATGMVDDHLQTCWVPRAPE"
     gene            complement(14506..14829)
                     /locus_tag="VF874_11065"
     CDS             complement(14506..14829)
                     /locus_tag="VF874_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX9176586.1"
                     /translation="MTLILMYLLVLVLVAGLLFVIASAVFGRGEPLPPLPRATTATAL
                     PASGVTGADVDAVKFTQTLRGYKTSEVDWVLDRLSREIELLRGQVSALRALVDDDAEP
                     VPDGR"
     gene            complement(14946..15887)
                     /locus_tag="VF874_11070"
     CDS             complement(14946..15887)
                     /locus_tag="VF874_11070"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX9176587.1"
                     /translation="MTTAPERTDVATDKWLAERSWPRPNWTVAELEAAKAGRTVSVVL
                     PALNEEETVASVVETILPMLGGLVDELVVLDSGSTDDTAIRALTAGARVVSREEAVPE
                     LLPQPGKGEALWRSLAATSGDIIAFVDSDLIDPDPMFVPNLIGPLLTADGVHLVKGYY
                     RRPLMVSGREDENGGGRVTQLVARPLLAALRPELSCVLQPLGGEYAGTRELLTSVPFA
                     PGYGVEIGLLIDTYDRLGLDAIAQVNLGVRAHRNRPLTELSAMSRQVVATLLSRCDVN
                     DSGVGLTQFFADGADFTPRVSEVCLDDRPPMNTLRPR"
     gene            complement(15884..16708)
                     /gene="folP"
                     /locus_tag="VF874_11075"
     CDS             complement(15884..16708)
                     /gene="folP"
                     /locus_tag="VF874_11075"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9176588.1"
                     /translation="MAIVNRTPDSFYDRGSTFTDDAAKAAVHHAIDEGADVIDVGGVK
                     AGPGQAVDSDEEIARVVPFIEWLRSAYPQQLISVDTWRATVAKQACAAGADLINDTWG
                     GVDPGLPEVAAEFDAGLVCSHTGGAAPRTRPFRVSYGHTVSGVVDDVIAEVTTAAERA
                     VAVGVARDRILIDPTHDFGKNTYHGLALLRHAEDLVNTGWPVLMALSNKDFVGETLGV
                     GLTERLEGTLAATAVTAAAGVRMFRVHEVEHTRRVLEMVAAIQGVRPPTRTVRGLA"
     gene            complement(16784..18586)
                     /gene="fadD6"
                     /locus_tag="VF874_11080"
     CDS             complement(16784..18586)
                     /gene="fadD6"
                     /locus_tag="VF874_11080"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase FadD6"
                     /protein_id="HEX9176589.1"
                     /translation="MAKNGEESREEREKSTVGLIDIASRVPGLIMDAPAIMQGVLTGF
                     MARPSAKTSIGKVFQDRAGRYGDKTFIKFQDETVTYREANETANRYAAVLAARGVGRG
                     DVVGILLKNSPRAVLLMLAVVKLGAIAGMLNYNQRGKVLAHSVGLLGSVVLIGEDELL
                     GEVKESGADVSDALSLDELDRLAADAPTDNPNVTSSILAKDKAFYIFTSGTTGMPKAS
                     VMTHYRWLRALGGFGGIGMRLNSNDILYCCLPLYHNNALTVAVSSVLSAGATLALGKS
                     FSASKFWDEVIGCEATAFVYIGEICGYLLNQPEKDTDCKHKVRVIAGNGLRPGIWDEF
                     TTRFCIPRVCEFYSASESNTAFVNVLNIPKSAGICPTPVAFVEYDHESGDPVRDDKGR
                     VKKVKSGEPGLLLSKVSNFQPFDGYTDKEATEKKLVRDAFKKGDVWFNTGDLMRSQGF
                     GHAAFSDRLGDTFRWKGENVATTEVEHAVQQDSNVEDATVYGVGVEGAGGKAGMAAIQ
                     LKDGADFDGKALAKTVYENLPPYAMPLFVRVVKELEKTSTHKTKKVDLAKQGYGEDVE
                     DPIYVLKNRDEGYVEFYDEYPEEVKAGKAPTSNK"
     gene            complement(18638..19222)
                     /locus_tag="VF874_11085"
     CDS             complement(18638..19222)
                     /locus_tag="VF874_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9176590.1"
                     /translation="MSDSVESRPEWAVCVYCASGPRHPELLALAAKVGEAIADRGWTL
                     VSGGGNVSAMGALAAGARAHRGRTVGVIPKALVHKEMADVDADELVITDTMRERKQVM
                     EDRSDAFIALPGGIGTLEELFETWTAGYLGMHEKPVVLLDPDGHYDGLWAWLESLVEA
                     GYVHPGALDRIVVTRDVEGALAVCAPIEEPLAPS"
     gene            19309..20541
                     /locus_tag="VF874_11090"
                     /pseudo
     CDS             19309..20541
                     /locus_tag="VF874_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788270.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(20766..21833)
                     /gene="dapE"
                     /locus_tag="VF874_11095"
     CDS             complement(20766..21833)
                     /gene="dapE"
                     /locus_tag="VF874_11095"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX9176591.1"
                     /translation="MGLDLSGDPIALTAALVDIPSESRQEARIADEVEAALREQTSGF
                     EIVRHGNAVLARTQLGRPTRVLLAGHLDTVPIADNLPSRREGDELHGCGTVDMKSGDA
                     VFLHLAATLDGPSRDLTLVFYDCEEIEAAANGLGRIEHELPDWLDADVAILGEPSGGF
                     IEAGCQGTLRVVVTAGGTRAHSARPWMGDNAIHKLGTVLDRLAGYDARHVDIDGCTYR
                     EGLSAVRIEGGIAGNVIPDHASVTVNFRFAPDRNLEQALQHVHEVLDGLDVRIEQTDA
                     AAGALPGLSHPAAAALVQAAGGQVRAKFGWTDVARFAARGIPAVNYGPGDPSLAHRAD
                     ERVPVGQITAAVEMLRDYLSS"
     gene            21883..22281
                     /locus_tag="VF874_11100"
     CDS             21883..22281
                     /locus_tag="VF874_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9176592.1"
                     /translation="MPHEAEFDDVRRLIGEFGSRATLVTVTESLRPHVVTAMIGIDGD
                     RLVAHVGSRTRSNVIDHPGLTVVWNPVDDGEYQLILDGTAEHVGEPNDRDVSTLRIAV
                     VGGILHRLAGLPEGPPTCRSLDAGAPRSNV"
     gene            22253..23230
                     /gene="dapD"
                     /locus_tag="VF874_11105"
     CDS             22253..23230
                     /gene="dapD"
                     /locus_tag="VF874_11105"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX9176593.1"
                     /translation="MPGRHAVTSSPVTSAAGVGLATISADGTVLDVWFPAPEIKVDDP
                     AGTARLSVAEVPDELAELAGRDDDRGVETALVRTVIGALADPPADAYDAYLRLHLLSH
                     RLIKPHDANVDGIFGVLTNVVWTNHGPCAVEGFEVVRARLRRRGPVTVYGVDKFPRMV
                     DYVIPTGVRIADADRVRLGAHLAPGTTVMHEGFVNFNAGTLGASMVEGRISAGVVVGD
                     GSDVGGGASIMGTLSGGGTQVISIGQRCLLGANAGLGISLGDDCVVEAGLYVTAGTKI
                     QTPDGATVRARELSGANNLLFRRNSLTGAVEVVSRDGQGIALNKALHAN"
     gene            complement(23250..23597)
                     /locus_tag="VF874_11110"
     CDS             complement(23250..23597)
                     /locus_tag="VF874_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9176594.1"
                     /translation="MGLTFDEICIDAADPTALGQWWSQALGWPQDVDEDGDVRLTAPP
                     GSGPNWLFLQVDDRRIVKNRIHFDFRPDDQRAEVERLVGMGARHVDIGQGEQSWVVLA
                     DPEGNEFCVLSER"
     gene            complement(23588..>24811)
                     /locus_tag="VF874_11115"
     CDS             complement(23588..>24811)
                     /locus_tag="VF874_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9176595.1"
                     /translation="LAVTGCLIAGVPVVPVPADVGHGERRHILTDSGAQAWLGEAPDE
                     AEGMPHIPVRLHARSWHRYPEPSPDATALIMYTSGTTGPPKGVVLSRRAIAADLDALA
                     DAWQWTPEDTLVHGLPLFHVHGLVLGLLGSLRIGNRFVHTGKPTPEAYAGAGGTLFFG
                     VPTVWSRVVADRAAADALRPARLLVSGSAPLPVPVFERLAELTGHQPVERYGTSESLI
                     TVSTRADGERRPGWVGLPLAGVTTRLVDDDEATVANDGETVARLQVRGPTMFDGYLGR
                     PDATAAAFTDDGWYRTGDVAVIDADGMHRIVGRESTDLIKSGGYRIGAGEVETALLGH
                     PGVAEAAVVGLPDDDLGQRIVAFVVGDVAPSVLIEYVAQELSVHKRPREVRVVDALPR
                     NAMGKVIKKELTTWG"
BASE COUNT         3799 a         8360 c         8491 g         4161 t
ORIGIN      
        1 tctgtgcgga cttggggggt cgattgcatt aaccgtttga gccacgctag ggggcctgat
       61 gaacctgaat atctcctcgc tgcggcggta cgcctaccta ccggtgcgca ggctgggcct
      121 gttcgtcgcg gtagtcgcgg cggtggcgat gagcgtcgtg accgtggctg taagtacttc
      181 ggggcgcgac gctcatgggt acggctcccg cccgatcgta gtcgctactg acaggcattc
      241 ggccacaacc accactatgg agataccgag cgccggggtt caggttaggt cagcgccgtg
      301 gcagggcaac ggctggccgg gaatgggacg atttcatggt ggaggctggc ccggacagta
      361 aggcgctcac cgaccaggtg cggtatttca ccaacgctcc ccgttgaccg cgttcgcggc
      421 aatgatggcg acccaatcgt ggacgccgaa gtcgtcgagc ccgagcctga ccgcaccagt
      481 ttgtcgcctg gctcgaaccc ctggaccgcg ccggcttggg tgccaagacc gcggtcagcg
      541 tggaggtggg cacgccggcc tacatcgagg tgttcggaaa ggtcgccgac acgttggcgg
      601 cgcgccgtcg cgggctggcc gggatgccgc tgggcatcga caccgcgcgc cgctgaaccg
      661 tctccgccgc gggcgaccta cgtcgcatcg gtgtcgaatg gggttgcgcc gcttgccgtc
      721 tcgggcttag tcacgggcgt gtccgggggc aggtcgacgg gcttgtcgaa cttgcccgtg
      781 aggatcgagt cgtcgccgtc gagcagatgc ttggtcaatg ccgcgcgcgg cgacatcccg
      841 cgaagcttct gcagttcggc aagcggctcg cgcaggtcgt cgaagtctgg gcccaactcg
      901 tcgcgcaggc tcttggtggc accgctgacg tagtcgcgag cctggcgcag cgcgttcgcc
      961 gaccagcgga tcgcggcggg caaccgctcg gggccgagga ccaccaggcc gattaccacc
     1021 agcacgagca tctcgcccca gccgacgttt gagaacatca gttgtcgggg gcgggagtga
     1081 ccgtcagcgt cacgtgccgg ccgtcgcgca ccacctcgat cggtgcgggt tgtccgattg
     1141 tcagcgcacg gaccgcgacc acgaactcgt cggcgtcggc gacggtccga tcgccgacct
     1201 tcaccaccac gtcgttctcc agcacaccgc ccttttcggc cgggccaccg gctttgacgt
     1261 tggcgacctg cgcgcccgac gccaccgagt tgctgaccga gcgggcggtc agaccgaggg
     1321 tggggtggac gatcttgccg tccttgatca acgcctccac gacagccttc acctcgttga
     1381 tggggatcgc gaagccaagc ccacttgcgc tgtcggacaa cgactttccg gcggtattga
     1441 tgccgatgat gtgagagttc atgtcgatca gcgggcctcc ggagttgccg tggttgatcg
     1501 cggcgtcggt ctgcagggcg tcgataacgg tgtcggtgtc ggagccttcg cccgacagcg
     1561 gcaccggccg gtgcagcgcg ctgatgatgc ccgatgtgac ggtgctgcgc aggccgaggg
     1621 gcgcgcccgc ggcgatcacc tcgtcgccca catgcacctt gtcggagtcg ccgaactggg
     1681 ccaccgtcag gttgttgacg ttgtcgacct tcaggaccgc caggtcggtc ttcgggtcgc
     1741 ggccgacaag gttcgccggc acctccttgc cgtcgttgaa caccaccgtg atcttgaact
     1801 ggctcgggtt gttggccgcc tcggagatca cgtggttgtt ggtgacgatg tagccgcgcc
     1861 cgtcgacgac aacaccggag ccctgcgagc cctcctggtc actaagcgct tcgacggtga
     1921 cgacggaatc ggccaccgcg gccgcgacct gagcgaaccg gccctccggc gcctggccgc
     1981 cgctggtcga cagcgtgacc ttggatgtcg tgaacgcctc gacgacctcc gcggtcttgc
     2041 ggcccaccca gccgccgacg aacgcgaggg ccagcgcgat caccgccagc accgcaagcg
     2101 cgcggtagga cacctttccg ccgaacagca catcgcgcac gccgagcttg ccggtggtgg
     2161 cgctcgccct ggccggttgt ggcttgggca gcgccggcgt ggtcagtgat gcagcggcgt
     2221 tcgggtcgcg ccacgggtct gcggcgaggt cggcgtcgtc gtcccgttcg ccgtcgaggg
     2281 ctcccacgtc ggaagggtgg cgctgcagcg actcaccgcc gctgtacggg cggccgaacg
     2341 cctcggccag caccggatcc ggcggggcgt tgcggggggt gaactcgcct tggtcgcgat
     2401 gcttgtcggc gccgagaaac gacccgtcga agccggccgg tcggccgaag gcacggctgg
     2461 acgccgggtc gaccggcggc cgggaaatgg ggcgcggcgc gaggcgcgtg gaagcgttgc
     2521 cgtcgcccgg accgggcggc tgggtgccgt cgggattctc tctgttgggg ctcaactcgt
     2581 cctcttccgg gggtgcggcc ggcgtgggcc ctggccaggc acggcgtccg tttttctgct
     2641 cttcgcgcaa gcgcttatgg ccaatgaagt ggtgtccacc ctacctgcgc ttacggcgac
     2701 cgcgcggcac ccctaagtcg tcgggggcgg cggctcccgg ttcgtcgacg ggtttagatg
     2761 ctcttcgccc gagtggctca tcgacgtcat cggactgatg cgggatctgc gcgagagtgc
     2821 cgagcagcga cgtgggcacg tcgatcggcc gggagtcccg cagcgcggcg cgggcctgcc
     2881 gctgcccctc gacctcggcc gagcactgcg ggcacagcga caagtgatgc gccgcgcgca
     2941 gataagcggt catgcgcagc tccccgtcga cgaacgcggc gatcgcctcc gtcgacaagt
     3001 gctcggtgga gccaaattca cgaggcgccc cgacgggcgc gtcactctgc gaggcgaact
     3061 gggagggcag ccaggagaat gcgcggcgga agagatgccc tcgatcggcc acgatgcctc
     3121 ctttcggcgc ggcatgtatt gctcctcgcg tccacgaccc tgcccgtgcg cggctccctc
     3181 gaatgtagcg cgcgtcacgc ccgccgacag cagctcaggc cgacagcgat gggccgcttg
     3241 cgcgaagagc agaaggcagc gatgagccgc ttgcgcgaag agcagaagac ttggcgacca
     3301 gctcctcgct gctgccgtgc gcggcgaggt agtcgcgcag cgcctggcgt ccgcggtgga
     3361 tgcggctacg cacggtgccg agcttgaccc ctagcgtcgc accgatttct tcgtacgaca
     3421 ggccctcgat gtcgcacaac acgaccgccg cgcggaactc cggcggcagc gaatccagtg
     3481 ccgcctgcag gtcgggaccc agccgcgaat cgtggtagat ctgctcggga ttcggctcgt
     3541 cggcgggcac acggtcgtag tcgtcaggca gcgactccat ccggatccgc gcgcggcgac
     3601 gcaccatgtc caggaacagg ttcgtggtga tccggtgcag ccagccttcg aacgtgcccg
     3661 gctgatagtt ctgcagcgag cggaacacgc gaatgaacgt ctcctgcgtc agatcctcgg
     3721 cgtcgtgttg attgcccgac agccggtacg ccagccggta cacccggtcg gcgtgctggc
     3781 gcaccagttc gtcccatgac ggcatcgtgc tcttgtcgcc ggtggcgtcg aacaccgctg
     3841 tgccctgcgg ctcatcggtt tcctcgaccc aaccggagtc gaacgaccgc tcaaaatgcg
     3901 acatggtcgc cggcggcatc ggcgtcggcg aaagggtgcg ggtcgtggga tcctcccggt
     3961 ccagtggata aacctcaccg ccggtggaca gcggcaatac ttcttcgaag cggctaacgt
     4021 cgatttcgtc aggactattc ccgacccgcc agcccgcgtg aaccatgcgc ttaccgttcc
     4081 ccatcgcggt gtggccggca tatgagtgac ctgagtgttt cctgagaaac cctgccgccg
     4141 gcaatttctg ctgcgtggcg ggtcttctcg ggcgcgcctg ctcgggtccg actgcctttg
     4201 gttttacgct gcgggcatga ccggcacgaa gcttggaatc ctggggcacg cggaggactc
     4261 gatttcggag gacccgatcg tcgccgcggc gcgcgaacga gccgtcgacg ccggcgcggg
     4321 ggcggtgacg cccgccgtgg gcgcgctgct ctcggtgctc gcccggctct ccggagcgaa
     4381 ggcggtcgcc gaggtcggca ctggggccgg tgtcagcggg ctgtggttgt tgtcgggtat
     4441 ggctgacgac ggagtgctca cgacaatcga cgtcgagccg gagcatcagc ggctggccaa
     4501 gcaggcgttc accgaagccg gaattgcgcc gtcgcggacc cgggtgatcg ccggccgcgc
     4561 gcaggacgtg ctgcagcggc ttgccgacga gtcctacgac ctcgtgttcg tcgacgccga
     4621 cccggtggac cagccgcatt tcgtcatcga gggcgttcgg ctgctgcggc cgggcggcgc
     4681 gatcgtcata caccgcgccg cgctcggcgg ccgggctggc gacccgtcgg cagccgacgg
     4741 cgaagtgacc gcggtgcgcg aagcggcgcg gctgatcgcg gaggacgagc ggttgacgcc
     4801 cgtgctggtg ccgctcggcg acggcgtcct ggtcgccgcc cgcgactgag tgatgtccta
     4861 ggcggagtgc cgccgcccca cggcctcgtc gaggatgtgc ccgtagccgt tggccagcag
     4921 actgaggcgc agactcggcc caggtgggtt gtctgtcagc cagaccctga gcgccgcggc
     4981 ctgcttgtcg acggacgcgt cgtgcagatt caacatccgt agcagcgccg gcgcgctgcc
     5041 tgcgtgcgcg gaatcgcgtt gagttagccg gctgcgctgt agtggcgtgc tcatgttcac
     5101 cgccgatcga ttcactggtc accgccgata accaagcttg ccactgatgg acgggtcgcg
     5161 tgaccgattc ggcagtatcg acggggacga cggccgctcc ggtgccgaat tgagcgttgt
     5221 tgtttgctgg cgctaccgga tcggcaagcc ggtcagcgct cgggccatca cgagccgctg
     5281 aatctcgctg gtgccctcga agatcgtgaa gatcttcgca tcgcggtgca tgcgttccac
     5341 cgggtagtcg cgcgtgtagc cgttgccgcc gaggatctgg atggcctcgt cggtgacaaa
     5401 cacggcggtc tcgctggcga acagcttggc catagacccc tccgcggagg agaactgctt
     5461 gttgttgcgg tgcatccacc cggcccgcca gaccaacaga cgcgacgcgt cgatgcggct
     5521 cttcatgtcg gcgagcttga acgcgatcgc ctggaattcg ccgatcttgc ggccgaactg
     5581 ctcgcgctgg caggcgtagt cgagggcgta ctcgtaggcg gcacgcgcaa caccgagggc
     5641 catcgcgccg accgccgggc gggtgcgctc gaacgtcatc atcgcggcct ggctggaggt
     5701 ccgtttgccc tcacgggcct tggcgacgcg ctgctcgaaa cgctcccggc cacccaggat
     5761 cagccgaccc gggatgcgca cgtcgtcgag aaccacctca gcggtgtgcg acgcgcggat
     5821 tccatgcttt ttgaacttct gtccctgcga gagcccgggg gtgtcgggag ggatgacgaa
     5881 cgtcgcctgg ccacgggtgc ccaggtcggg atataccgat gcgacaacga cgtgcacgtt
     5941 ggcgatcccg ccgttcgtcg cccatgtctt ggtgccgctg agcacccatt cgtcggcggc
     6001 ttcgtcgtaa cgggctcgcg tgcgcaccgc gccgacgtcg gaaccggctc ccggttctga
     6061 ggagcagaac gcggccacct tgggatcgcc tgggctgccg aacatctggg gcaggaactc
     6121 acccagttgc tccggggtgc cgcttgcggc gagcgcggcc gcggcaagcc cggtgcccag
     6181 gatggacagc gcgatgccgg cgtcgcccca gaacgtttcc tcgaacaccg agagcattcc
     6241 gatgccgctg ggctccgcgg ccatctgcac gaacagctcg ggggtgtaca ggccgacttt
     6301 ggcggcctcg gcgatgatcg gccacggcgt ctgttcccgt tcgtcccact ccgatgcggc
     6361 cgggcggatc acctccgcgg cgaactcgtg cacccagtcg cgaacgtgca caacctcttc
     6421 cggcagctcc aatgagaatt ccgcggtcac cgtcgtcttc acctttccga ttacgccgca
     6481 ttgtgcgtca gccacaagag ttggcacatg gtcgaagcgg ccatacgatg tcgcgaccgt
     6541 ctgcgcctcg gcgagtactc aagagtaaac gcgtgtacac ccagaattgc aacgcgattg
     6601 tgaggagcgc caccattgac gctcccttga cgtgcaactg aacacacgtt tagcatattg
     6661 aacatgcgtt cagaggacat gacgacagcg gccagaatcc gcgatgcggc gattgagcag
     6721 tggggccagc acgggttcca tgtcggccta cgcgccatcg ccgaggccgc cggcgtgagt
     6781 gcttcgctgg tgatccacca cttcggatcc aaacaaggcc tgcgcacggc ctgcgacgac
     6841 tacatcgccg aggaagtccg cagcgcgaag accgagtcgc tgcggtcggc cgacccggcc
     6901 acctggttcg cgcagatagc cgagatcgag tcgtacgcac cgatgatggc gtacctggtg
     6961 cgcagcatgc agtccggcac tcagctggca aagacgttct ggcagcggat gatcgacaac
     7021 gctgaggaat acctggagga aggcgtgcgg gccgggacgc tcaagccgag ccccgacccg
     7081 aaggcccgcg cgaaattcct ggcgatgtcc ggcggcggag cattcctgct ctacctgcag
     7141 atgcacgaga acccgaccga catggcggcg gtgctgcacg actacggcga ggagatggtg
     7201 ctgcccgctc ttgaggtcta cagccacgga ctgatgaccg acagcacgat gtacgacgcg
     7261 ttcgtcgccc aaagagaaca agtaaggagc aaagcatgac tgaccggcag agtgacgtgc
     7321 ccgccgtcga aatccacggc ctgcgaaagt ctttcggccg gacgaaggca ctcgacgggc
     7381 tcgatctgac ggtcgagcgc ggtagcatcg ccggcttcct cggccccaac ggctccggca
     7441 agtcgaccac catccgtgtg ctgctgggcc tgttgcgcgc cgaggcaggc accgtccgac
     7501 tgctcggtgg cgacccgtgg cacgacgcgg tgtcgctaca ccgccgcgtc gcctacgtgc
     7561 cgggcgacgt gacgctgtgg ccgaacctga ccggcggcca ggccatcgac ttcctcgccc
     7621 ggctgcgcgg gggcgtcgac gcgcgccgcc gcgaccagct gatcgagcgt ttcgaactcg
     7681 acccgcacaa gaaggcccgg acctactcca agggcaaccg gcagaaggtg gcgctggttg
     7741 cggcgttttc ctgcgacgcc gagctgtaca tcctcgacga gccgacatcc gggcttgacc
     7801 cgttgatgga gagggctttt gggaactgcg tgcgcgaagt cgccgaccgg ggtgccgcgg
     7861 tcttgctgtc cagccacatc ctcgccgagg tcgaaaagct ctgtgacaca gtgacaatca
     7921 tccgttcggg aagcacggta cggtcaggtg gcctggccga gctgcgtcac ctgatgcgta
     7981 ccaccgtgaa ggtgcgcacc cgtgccgaca ccaacggcct gcacgacgca gcgttcgtcc
     8041 acgacttcag cagctccgat ggcgtcgtga cgttctcggt gaaccgccgc gatctcgacc
     8101 aagcgatgga ccggctgacc gcgctcggca tcgacgagct gtcggtctat cccgcatcgc
     8161 tggaggacat gttcctgcgc gaataccagg ggacgaaccg atgacggctg gcacccacgc
     8221 gcgcccgtcg gccgctccgc tgaccgggtg gccgaacctg ctgcggctgg cgctgcggcg
     8281 tgaccggatc tggctgtcgg tctggatctc ttcgctgacg ctgatgatgc tgtacgcgcc
     8341 gaatgcgatg aagctcgcgt atcccgaaga ggtacaacga tttgcgcggg tgaacctgct
     8401 gaagacgcca gccggaatga tgctgggcgg gccgatgttc ggcggcaacg aaaccgagct
     8461 cggcgccatg gtcgccaacg aattgatgtt gaccgtgatc atcgcggtgt caatcatggc
     8521 catcctcacc gtgatccggc acacccgcgc cgaagaggaa agcggcgcag ccgaattggt
     8581 gatgtcgtcg gtggtgggcc gctacgcccg cacgttcgcc gcgctggtgc tgatgggaat
     8641 ggtcaacggg atcctggcga tgacaatgac ggtagcgctc gcggctaccg ggctgcccgt
     8701 cgtagattgc gccgcaatgt gtttgggtat caccggggtg tccatggtgt ttggcgcggt
     8761 cgcagcggtc actgcgcagc tgtggcggca ggcgcgcacg gcgtcgggcg ccgcgatggc
     8821 ggtgctggcc gcggtcgcgc tgatccgcgg catcggcgac gtcatcgaca actcgggcag
     8881 cgcactttcc tggttctccc cgatcgcctg ggcgcagcag atgcggccgt tcgtcgacct
     8941 gcggtggtgg ccgttcgtgt tgctgatcct gctcgtcgtt gtgctggttg cggtggccgt
     9001 cgcgctggaa agccgccggc aatacgacga cggcgtcctg ccggccacgg gcgagcaccc
     9061 gaacgcgcac gcaatacgcg gggtgttcgg acttcacgag accctgcagc gcgggcaggt
     9121 gatcgggtgg tcggtggctc tgctgctctc cggggcggcg ttcggttcga tgacgaagtc
     9181 gctacaggat gccgcgaaaa gcaacccgtt gattgcccgg gtgctggccg cggagggact
     9241 cgacggcatc tacacgacga tgacgcagtt cctcgcggcc gcgacgaccg cgttcgtcgc
     9301 cgccgcggtg ctgcgactgt cccgcgacga gcagtcggga ctggccgaac cggtgctcgc
     9361 cgggtcggtg tcgcgatggc gctggctgct cacagcggtc acggccgcag tgctgtcctc
     9421 ggcagtgttg atgctgtgcg cggggttcgg caacgggttg ggcgccggca tcacgctcgg
     9481 cgagccgcca acggtcgcgc ggctgactgc ggccggactg gccttcgtgc cggccatggc
     9541 ggtggtcgcg gccgtcgcgg cgctggccgt cgccgtgaaa cggccgtgga tcgcctggct
     9601 gttcgtgacg tacatcgtgc tggcgctgta tctgggcgct ctgctgcggc tgccgcgctg
     9661 gctggtcgac gcgtcgcccg tcggccgcac cacggccccg acgcacgttc cggtgactgc
     9721 gctgctgatg atgatcggcg tcgcaatcgc attgaccgca gtggccggcg tcatctaccg
     9781 gcgtcgagat gtggtgtgag agaaaaagcc atggcgaccg cacttcgcat ggcgggaaca
     9841 tcgctgctcg gactggcggc tttcgggttg ctgctgttca tgccggcagg gacgttgcgt
     9901 tattggcagg catgggtttt catcgccgtg ttcacgatct gcacgctcgt gccgagcact
     9961 tatctgctgg tgaaggaccc cgccgcgttg cagcgccgca tgcacgcggg gccggcggcg
    10021 gaaaccagaa cgacccagaa gatcgtcatc gccagcgcgt tcctgttggt gccggcggtg
    10081 atggtgtcca gcgccttcga ccatcgcttc ggctggtctc cggtgccgac tgcggtgtcg
    10141 ttgattggcc aggcgctggt ggcaatcgga ctcggcatca cccagtacgt catcatgcag
    10201 aacagttacg cggcggccac catcaccgtc gagtcgggcc agaaggtggt gtcctccggg
    10261 ctgtacagcc ttgtgcggca cccgatgtac gtcggaaacc tgatcatgat ggtcggcatc
    10321 ccgctggcgc tcggctcgta ctggggattg ctgatcctcg tccctggcgt agttctgctc
    10381 gcggtgcgga tcctcgacga ggaacgagcg ctggcgcagg agttggccgg ataccgtgac
    10441 tacatgcaga aagtgcacta tcggttagtg ccgcacatct ggtgatggaa cttctcacgg
    10501 gattcggtct ggccacggcg gccgggctca acgcctacat cccgatactg atcctgggcc
    10561 tgctcgcccg cttcacagat gtgatcacgc tgccgcacgc atggtcgtgg ctggagaacg
    10621 gctgggtgct gacgatcgtc gcggtgttgc tggcggtcga ggtagtggcc gacaagatcc
    10681 cagcactcga cagcgtcaac gacgcggtgc agaccgtcgt ccgaccgact gcgggcggca
    10741 tcgtgttcgg ctcggggacc gccgcacaga ccgtcgccgt caccgatcct ggtgcgttcg
    10801 ccaggtcagg ccaatggatt ccgatcgcga tcggaatcgt cgtcgcgttg gtcgtgcacc
    10861 tgaccaagac gacggtgcgc ccggtggcca acgttgcaac ggccggggtc gccgcgccgg
    10921 tgctcagcac gatcgaggac atcaccagcg tcgggctggt ggtggtcgcg atcgtgttgc
    10981 cggtgctggt gctcgtggtg gtcgccgcgc tggtgtgggc cgcggtgaaa ctgctgcgcc
    11041 gacgacggcg agcacgtcgc cgagactgaa gccagggtcg tgaattccac ggatcaacga
    11101 cccggaattc ggtttcggcg caagccggct agatccagat gcccttgccg accgcgacga
    11161 cgccgccggc gctgaccgcg aagcgttccc ggtcgcgctc gaggtcgacc ccgaccatct
    11221 cgccgggacc cacgacgacg ttcttgtcga gaattgcgtg ccgcaccgcc gcgccgcgcc
    11281 cgacgcgcgc gccgggcatc aacacgctgc cttcgacgat cgcgccttcg tcgacgacga
    11341 cgttggacga cagcaccgag ttgcgcaccg acgccgcgga cacgatgctg cccgcgccga
    11401 ccactgactc ctgcgcggag ccgccgttga cgaactttgc cggcgccagg ttctccgact
    11461 ccccgcgaat aggccagcgc ttgttgtaga ggttgaagat cgggtgcacc gacaccaggt
    11521 ccatgtgcgc gtcgtagaac gcgtcaagcg tgccgacgtc acgccaatag ccgcggtcgc
    11581 gttcggtggc gccgggcacc tcgttgtcgt cgaagtcgta gaccgcggcg cgcccgtcat
    11641 cgactagacg cggaatgatg tcgccgccca tgtcgtggtc ggagttgtcg tcgtcggcgt
    11701 cggcgcgcac cgcgtcgatg agcaccttgg tggtgaagat gtagttgccc atcgacgcga
    11761 aggtttgctc ggggtcgtcg tgcgtgccgg gcgggtcgga cggcttctcg acgaagtcgc
    11821 ggatgcggcc ggactcgtca gagtcgatgc agccgaacgc cgttgcctcc ttgcggggca
    11881 cacggatgcc cgcgaccgtt gcgcccgcgc cgctttcgat gtggcgggtc agcatctgct
    11941 cggggtccat ccggtacacg tggtcggcac cgaacacgac gatgtagtcg gggtcttcgt
    12001 cgtagatcag gttcatcgac tggtagatcg cgtcggcgct accggtgtac caccgcgggc
    12061 cgagccgttg ctgcgcgggc accggcgtga tgtattcgcc ggccaggccg gagagccgcc
    12121 agttctgcga gatgtgccgg tcgagggaat gggacttgta ctgggtgagc acgcagatgc
    12181 gcagataccg ggcgttgacc atgttcgaca acacgaagtc gatgagccgg taggcgccgc
    12241 cgaacgggac tgcgggtttg gcccggtcgg cggtcagcgg atacagccgc ttaccttctc
    12301 caccggcgag gacaacgccc aacacatgtg gcaattccct catgcctaga acctatccgt
    12361 actcgttgac tgcggccaga aattctccgg aattcacccg ggtcaaacgc cacagtcgac
    12421 gacgtgggtg catcgtccgc ttgcgggcac taccgtcatg acgatgcggg tggcgatgat
    12481 gacgcgggag tatccgccgg aggtctacgg gggcgccggg gtccacgtca cccagctcgt
    12541 cgctcagttg cgcaagctgt gcgaggtgga cgtgcactgc atgggcgcac cccggcacgg
    12601 cgcgtacgtt caccagcccg acccggcgct gcgcggagcc aacccggccc tgacgacact
    12661 gtcggccgac ctggtgatgg ccaactccgt cctcgccgcc acggtcgtgc acagtcacac
    12721 ctggtacacc gggctggccg gtcacctggc cgcgctgctc tacggcatcc cgcacgtgct
    12781 caccgcgcat tcgctggagc cgctgcggcc gtggaaggcc gagcagctgg gtggcggtta
    12841 tcgggtgtcg tcgtgggtgg agcgcaccgc cgtcgagacc gccgacgcgg tgatcgcggt
    12901 gagctccggg atgcgtgagg acctgctggc cacctatccc gcgctggacc cgaacgtggt
    12961 gcatgtggtg cgcaatggga tcgacaccga cgtgtggtgt ccggtcgcgg acgcgaggag
    13021 caaaacaggc accgcggacg cgaggagcaa gaaagaagaa gagccgacac tgggcgaatc
    13081 ggtactcgcc gagcttggcg tcgacgtcga gaagccgatc gtcgcgttcg tcggccggat
    13141 cacgcggcag aaaggcgtgg cacacctggt tgcggccgca caccggtttg cccccgaggc
    13201 gcagttggtg ttgtgcgccg gcgcgcctga cacccccgag atcgccgccg agatcaccga
    13261 ggctgtgcag aacctgtcgc gcgcccgcag tggcgtgttc tgggtacgag agatgctgcc
    13321 gatcggcaag gttcgcgaaa tactctcagc ggcaacggtt ttcgtgtgcc cgtcggtgta
    13381 cgagccgttg gggatcgtca atctcgaggc aatggcctgc gcgacggccg tggtcgcctc
    13441 cgacgtgggt ggcattccag aagtggtggc cgacggggac accgggctct tggcgcacta
    13501 cgacgcgggt gacccgtcaa gtttcgagac gcggttggcc gacgccgtca acacgctcgt
    13561 cgcagatccg gacagagccg aacgcttcgg ccgggcgggc cgtcaacggt gcatcgacga
    13621 gttttcgtgg gcgcgcatcg cccaacagac actggagatc taccgcaagg tgagcgccag
    13681 ctagctggtg acgcccttga gctcgtcgcc caacgcggcc gcctcttcgg gagtgagttc
    13741 gacgacgagc cggccaccgc cctccaacgg tactcgcatc acgatgccgc gcccctcctt
    13801 ggttgcttcc agtggaccgt caccggtccg gggcttcatc gccgccatcg agcgctccct
    13861 ccagactgca cgttcacgct cctattgttc cttatccagc agccttcgtg tgcaaagacc
    13921 ccgctaaccg gccttattcc ggcgcccgcg gcacccagca tgtctgcagg tggtcgtcca
    13981 ccatgccggt ggcttgcatc agcgcatacg cggtggtggg gccgacgaac cggaatccgc
    14041 ggcgcttcaa ctccttggcc atcgcgatgg actccggact caccgccgcc acgtcggcag
    14101 cggtggccgg cctcggccga ggaggcggcg gcgcgaacga ccacagcaat tcggacaggt
    14161 ccgcatcgag ccccgtggcg gcgcgcgcgt tcgcgatcgt ggcatcgatc ttcgcgcggt
    14221 tgcgcacaat tgaagcgtcg gccaccagcc gggcgacatc gcggtcggtg tagcgggcga
    14281 ccttctcgac gtcgaagccg tcgaaggcgc gccggaagtt ctcccgctta cgcaggatcg
    14341 tcagccacga cagcccactt tggaacgcct ccaacgagat tcgctcaaac agcgcatccc
    14401 gtccgtgcag tggccggccc cactcgaagt cgtggtagtc gcggtacatc gcggcttgag
    14461 cgccgccgct ttgcgcccac gagcagcgga cgcggccgtc gtcggtcacc ttccgtcggg
    14521 gacaggctcg gcgtcgtcgt caaccaacgc acgcaacgcc gacacctgac cgcgcagcaa
    14581 ctcgatctcg cggctcagcc ggtcgagcac ccagtccacc tcgctggtct tgtatccgcg
    14641 cagggtctgc gtgaacttga cggcgtcgac gtcggcaccg gtgacacccg aggcgggcag
    14701 cgcggtggca gtggtggcgc gcggaagcgg cggcagcggt tcgccgcggc cgaacaccgc
    14761 gctggcgatc acgaacagca gcccggcgac gagcaccagc accagcaagt acatcaggat
    14821 cagcgtcacg acacagatcc tgcctcatgc gacggacgaa atagcccatc ggccgtgcta
    14881 gcgagagtga cgtttggcag gcctgcactt tctctgcgaa acgtcagttt cggcgctgaa
    14941 gtcgcctacc ggggccgcag cgtgttcatg ggtggacggt cgtccaagca gacctccgag
    15001 acccgcggag tgaagtcggc gccgtcggcg aagaactgag tgaggccgac cccggaatcg
    15061 ttgacgtcac agcgcgacag cagcgttgcg acaacctggc ggctcatcgc ggagagctcg
    15121 gtcagcgggc ggttgcggtg cgcccgcaca ccaaggttga cctgtgcgat cgcgtccagc
    15181 ccgagccggt cgtaggtgtc gatcagcagg ccgatctcga ccccgtagcc gggtgcgaac
    15241 ggcaccgacg tcagcagctc gcgggtgccg gcatattcgc cgccaagcgg ctgcagcacg
    15301 caggacagct cggggcgcag cgcggccagc agcggccgcg cgaccagttg ggtgacccga
    15361 ccgccgccgt tctcgtcctc gcggccactg accatcagcg gtctgcggta gtaacccttg
    15421 accaggtgca cgccgtcagc ggtcagcaac ggcccgatta ggttcggcac gaacatcggg
    15481 tccgggtcga tcaggtcgga atcgacgaac gcgatgatgt cgccgctggt ggccgccagc
    15541 gaacgccaca gcgcctcacc cttgcccggc tgcggtaaca gctccggcac agcctcttcg
    15601 cgactgacca cccgtgcgcc tgcggtgagc gcgcggatcg cggtgtcgtc ggtggagccg
    15661 gagtcgagca cgacgagctc gtcgaccagg ccgccgagca tcggcaagat ggtctcgacg
    15721 acggacgcga ccgtctcttc ctcgttgagc gcgggcagta ccaccgaaac cgtccggccc
    15781 gccttggcgg cctcgagctc ggccacggtc cagttcggcc ggggccagct gcgttcggcc
    15841 agccatttgt cggtggcgac gtccgtgcgc tccggtgcag tcgtcatgcc agtcccctca
    15901 ccgtgcgcgt cggcgggcgc acaccctgga tcgccgcgac catttccagc acgcgccgag
    15961 tgtgttctac ctcgtgcacc cggaacatcc gcaccccggc cgcggccgtg accgcggtgg
    16021 ccgccagcgt gccttccagg cgttcggtca aacccacacc cagagtctcc ccgacgaagt
    16081 ccttgttgct cagggccatc agcaccggcc atccggtgtt aacaagatct tccgcgtgcc
    16141 gcaacaaagc gagcccgtgg taggtgttct tgccgaaatc gtgggtcggg tcgatgagga
    16201 tccggtctcg ggccaccccc accgcgaccg cccgctcggc ggccgtcgtc acctcggcga
    16261 tgacgtcgtc gacgacgccg ctgacggtgt ggccgtagct gacccggaac ggccgcgtcc
    16321 ggggcgccgc cccgccggtg tgagagcaca ccaggccggc gtcgaactcg gcggccacct
    16381 cgggcagtcc cgggtcgacc ccgccccagg tgtcgttgat caggtcggcg cccgccgcgc
    16441 atgcctgctt ggccaccgtc gcgcgccacg tgtcgacgct gatcagctgc tgcgggtacg
    16501 cgctgcgtag ccattcgatg aatggcacca cccgcgcgat ctcctcgtcg ctgtcgaccg
    16561 cctggccggg gcctgccttg acgccgccga cgtcgatcac gtcggcgccc tcatcgatcg
    16621 cgtggtgcac cgccgccttg gcggcgtcgt cggtgaatgt ggagccgcgg tcgtagaacg
    16681 agtccggggt gcggttgacg atcgccatga tcagcgcacg atcgccggcc accggacggc
    16741 cgcagaacgt cgactccacc cctccagggt gccaggcgct ctgctacttg ttgctcgtgg
    16801 gcgccttccc ggccttcact tcctcgggat actcgtcgta gaactcgacg tagccctcgt
    16861 cgcggttctt cagcacgtag atcgggtcct cgacgtcttc gccgtagccc tgcttggcca
    16921 ggtcgacctt cttggtcttg tgcgtcgaag ttttctccag ctctttgacg acgcgcacga
    16981 acagcggcat cgcatacggc ggcaggttct cgtacaccgt cttggccagc gccttgccgt
    17041 cgaagtcggc gccgtccttg agctggattg cggccatccc ggccttgccg cccgcgccct
    17101 cgaccccgac gccgtagacc gtggcgtcct cgacgttgct gtcctgctgc acggcgtgct
    17161 cgacctcggt ggtggccacg ttctcgcctt tccaccggaa cgtgtcgccg agccggtcgg
    17221 agaacgccgc gtggccgaag ccctgcgaac gcatcaggtc gccggtgttg aaccagacgt
    17281 cgcccttctt gaacgcgtcg cgcaccagct tcttctcggt ggcctctttg tcggtgtagc
    17341 cgtcgaacgg ctggaagttg ctgaccttgg acagcagcag gcccggctcg ccgctcttga
    17401 ccttcttgac tcggcccttg tcgtcgcgca ccgggtcgcc gctctcgtgg tcgtactcga
    17461 cgaacgccac cggcgtcggg cagatcccgg cgctcttggg gatgttcagc acgttgacga
    17521 acgcggtgtt ggactcgctg gccgagtaga actcgcagac ccgcgggatg cagaaccgcg
    17581 tggtgaactc gtcccagatg cccggccgca gcccattgcc tgcgatcacc cgcaccttgt
    17641 gcttgcagtc ggtgtccttc tccggctggt tgagcaagta gccgcagatc tcgccgatgt
    17701 agacgaacgc ggtcgcttcg cagccgatga cctcgtccca gaacttcgac gccgagaacg
    17761 acttgcccag cgccaacgtg gcgcccgcgc tcagcaccga cgacaccgcg accgtgagcg
    17821 cgttgttgtg atacagcggc aggcagcagt acaggatgtc gttgctgttg agccgcattc
    17881 cgatgccgcc gaagccgccg agggcccgca gccagcggta gtgcgtcatc acgctggcct
    17941 tcggcatgcc ggtggtgccc gacgtgaaga tgtagaacgc cttgtccttg gccaggatcg
    18001 acgacgtgac gttcgggttg tcggtcgggg cgtcggcggc gagccggtcc agttcgtcga
    18061 gggaaagcgc gtcgctcacg tcggcgccgg actccttcac ctcgcccagc agctcgtcct
    18121 cgccgatcag aaccaccgag ccgagcaacc cgacgctgtg tgccagcacc ttgccgcgct
    18181 ggttgtagtt gagcatgccg gcgatcgcgc ccagcttgac cacggcgagc atcaacagca
    18241 ccgcgcgcgg cgagttcttc agcaagatcc cgacgacgtc gccgcggccg acgccgcgag
    18301 ccgccagcac cgcggcatac ctgttggcgg tctcattcgc ctcgcggtac gtcacggtct
    18361 cgtcctggaa cttgatgaac gtcttgtcgc cgtagcggcc ggcccggtcc tggaacacct
    18421 tgccgatcga ggtcttcgcc gacggccggg ccatgaaccc ggtcagcacg ccctgcatga
    18481 tcgccggggc gtccatgatc agcccgggca cccggctggc gatgtcgatc aggccaacgg
    18541 tcgacttctc tctttcttcg cgcgactctt cgccattttt cgccatgcgc ttcatcttcc
    18601 ccccttcgcg tcccccacaa actggcgaaa agcgatgtca ggacggcgcc agcggctctt
    18661 cgatgggggc acagaccgcc agcgccccct cgacgtcgcg ggtcaccacg atccggtcga
    18721 gcgcgccggg gtgcacatat cccgcctcga ccagactctc aagccacgcc cacaggccgt
    18781 cgtagtggcc gtcggggtcg agcagcacga ccggcttctc gtgcatgccg agatagccgg
    18841 cggtccaggt ctcgaacagc tcctcgagcg tgccgatgcc gccgggcagc gcgatgaacg
    18901 cgtcggaacg gtcctccatc acctgcttgc gttcccgcat tgtgtcggtg atgacgagct
    18961 cgtcggcgtc gacgtccgcc atctccttgt gcaccaatgc cttcgggatc accccgaccg
    19021 tgcgtccgcg gtgtgcgcgg gcgcctgcgg ccagtgcacc catcgccgag acgttgccgc
    19081 ccccggacac cagagtccag ccgcggtcgg cgatcgcttc gcccactttg gctgccagcg
    19141 cgagcaattc ggggtgccgc gggcccgacg cgcagtacac gcagaccgcc cattcaggcc
    19201 gcgactcaac agaatccgac acacccgcca aactagaccc ataggatgcg gcgagtgacc
    19261 gacgataaca gcgtcgatga gcgctcgcgc gaagagcatc gagccgaagc cgccgcagag
    19321 gcgctgcggc tcggtgacat ggagtcgagt gagaatctcg cacgatccgc gctccagcag
    19381 tcggagtcgc tggctgcccg gctgaccctg gcccaggccc ttgcctggca gggccgcggg
    19441 cgggaggccg ccgaaaccct cgccgcggtc gaccccgacg ggctttcgga gaccgacctg
    19501 atggcgtggg cgctgccgcg ggccgccaac cagttctgga tgctcagcca gccgacccag
    19561 gctgtcgctt tcctgcagac cgttcgcaac cgggtctcgg caccgggcgc gcgcgccact
    19621 ttggatgggc tcgccgccac cttcgcgatg aacgccggca cgccgctgcg ggcattgcgc
    19681 ctcgccgacg aggtgctggc gtcgccgtac gccgacgaca tcgcggccgg ctgggcggcg
    19741 tcgaccgccg cgctgtcgtc ggcgcggacg ggccggttca gccgcgtcga tgaactggcg
    19801 gcacgggcgc agtccgccgg ccatcccggc ctgttgcggt tcaccagcgg attcggccgg
    19861 atcaccggat tcctgatggg gggccgactc gacgaggccc gggcgctggc gcagcgctac
    19921 accgactctg ccgagcggca gcagcccggc catgcgatcg gcggggtgct ggtctcgtat
    19981 gtcgcgatcg cacagggcga cttcggcgcg gcagcggcgc tgctggagcg ggccgctgac
    20041 gcattggcgc cgacgggcta ctcgtgggga ccgctggcgc tgatgctgct ggcccaggcg
    20101 ctcggccagc ggggcgacca gatcggcgcc gcgaaggcgt tgagtgcggc ggagtcgcga
    20161 cacggcatga agtccgcgct gttcacgccg gaactcgcgc tggcgcgggc ctggtcgaag
    20221 gcggcccgca atgacgtgca ggaggcgatc gacgcggcgc gtgatgccgt tcaggctgcc
    20281 gagcgcggtg gccagtcggc ggtggcgttg cgcgcgctgc aggatgcggc ccggctcggt
    20341 gacacccagg cggtctaccg ggcgcagcgg ttggccgggg aagtcgactg cgtgctgggc
    20401 cgcctaacgc tggcccacgc ccaggcgctc accgacggcg acggcgtcgc gctgaagcag
    20461 gttgccgaag agttggccga catcggcttg catcccgctg ccgccgacgc cgcccgccaa
    20521 gcggaccggg acggcccgta accgagcatc tcgtgttcat tcccggcgtc ggtggccgcg
    20581 actggctgtt cacgcgctga tcgaagaatc cggccaccgc acgccgatcg agcggccgca
    20641 ggcgttcact gcggtgatgc ggctgtggct gacgcgctga ggtgctcccg gtgtcgccga
    20701 gaatgaaacc atggtcgtga tcttggcgga tcaacgaccc ctggaattcg gtttcggcgc
    20761 atgcgctagc tgctgaggta gtcgcgcagc atctcgacgg ccgcggtgat ctgcccgacg
    20821 ggcacgcgtt cgtcggcgcg atgggcaagg ctggggtcac cgggaccgta gttgacggcg
    20881 ggaatgccgc gggccgcgaa ccgcgcgacg tcggtccagc cgaacttcgc gcgcacctgc
    20941 ccgccggcgg cctgtaccag cgcggcggcg gccgggtgtg acaaccccgg cagcgccccc
    21001 gctgcggcgt cagtctgctc gatccggaca tcgagcccgt cgaggacttc gtgcacgtgc
    21061 tgcagggcct gctcgaggtt gcggtccggg gcgaagcgga agttgacggt cacggacgcg
    21121 tggtccggga tcacgttgcc ggcgatgccg ccctcgatgc gcaccgcgct cagcccttcg
    21181 cggtacgtgc aaccgtcgat gtcgacgtgc cgggcgtcgt agccggccag ccggtccagg
    21241 acggtgccga gcttgtgaat tgcgttgtcg cccatccacg gtcgcgcgga gtgcgcccgg
    21301 gtgccgccgg ccgtgacgac gacgcgcagc gtgccttggc agccggcctc gataaagccg
    21361 ccggacggtt cgccgagaat cgcgacgtcc gcgtccagcc agtcgggcag ctcgtgctcg
    21421 atccggccca aaccgtttgc ggcggcttcg atttcctcgc agtcgtagaa caccagtgtc
    21481 aggtcgcggc tgggtccgtc gagcgtggcg gccaggtgaa ggaacaccgc gtcaccggat
    21541 ttcatgtcaa ccgtgccgca gccgtgcagc tcgtcgccct ctcgccggga cggcaggttg
    21601 tcggcgatcg gcacggtgtc gaggtgcccg gccaacagca cgcgcgtcgg gcggccaagc
    21661 tgtgtgcggg ccagcaccgc attgccgtgc cggacgatct cgaatccgct ggtctgttcg
    21721 cgcagcgcgg cctcgacctc gtcggcgatg cgggcctcct gccgcgactc actcgggatg
    21781 tcgacgagcg cggcggtcag tgcgatcggg tcgccgctca gatctaggcc cacggggatc
    21841 aaggctagtc gccagctatc gcggcagacg tacgatgcgg tgatgccgca cgaggccgag
    21901 ttcgacgatg tccggcgttt gatcggcgaa ttcgggtcgc gcgcgacgct ggtgacggtg
    21961 acggaatccc tgcgtccgca cgtcgtgaca gcgatgatcg gcatcgacgg cgaccggctt
    22021 gtcgcccacg tcggatcgcg gacgcgatcg aacgtgattg accatcctgg cctgaccgtc
    22081 gtgtggaacc cagtcgacga cggcgagtat cagctgattc tcgacggcac ggccgaacac
    22141 gtcggcgagc cgaacgaccg cgacgtgagc acgctgcgaa tcgctgtcgt cggcggcatc
    22201 cttcaccggc tcgccgggct accggaaggc ccgcccacat gccggtcgct ggatgccggg
    22261 gcgccacgca gtaacgtcta gccccgtgac ttctgctgcg ggcgtgggcc tggcgaccat
    22321 cagcgccgac gggacggtgc tcgacgtgtg gttcccggcg ccggaaatca aggtcgacga
    22381 tcccgcaggc accgcccggc tgtcggtggc cgaggtgccc gacgagctgg ccgagctggc
    22441 cggccgcgac gacgaccgcg gcgtcgaaac cgctttggtg cgaacggtga tcggcgcgct
    22501 ggccgacccg cccgccgacg cctatgacgc gtatctgcgg ctgcatctgc tgtcgcaccg
    22561 attgatcaag ccgcacgacg cgaacgtcga cgggatcttc ggcgtgctga ccaatgtggt
    22621 gtggaccaat cacggccctt gtgcggtcga aggtttcgag gttgtgcggg cacggctgcg
    22681 tcggcgcggc ccggtgaccg tctacggcgt cgacaagttc ccgcggatgg tcgactacgt
    22741 gataccgacc ggtgtgcgga tcgccgacgc cgaccgggtg cgcctcggcg cgcatctcgc
    22801 accgggaacg acggtgatgc acgagggctt cgtcaacttc aacgccggca ccttgggcgc
    22861 gtcgatggtc gagggccgca tctccgcggg cgtcgtggtc ggcgacgggt ccgacgtggg
    22921 cggcggcgcg tcgatcatgg gcacgctgtc cggcggtggt acacaagtga tttcgatcgg
    22981 ccagcgctgt cttctcgggg cgaacgcggg cctgggcatt tcgctcggcg acgactgcgt
    23041 ggtggaggcc ggtctgtacg tgacggcggg taccaagatc cagacacccg acggcgcgac
    23101 ggtcagggcg cgagagctct caggagcgaa caaccttctg ttccggcgca attcgctgac
    23161 cggcgcggtc gaggtggtgt cccgcgacgg ccaaggtatc gcgctgaaca aggccctgca
    23221 cgccaactaa tttgccgcga gcgcagaagt cagcgctccg acagcacgca gaactcgttg
    23281 ccttcggggt cggccagcac cacccagctc tgctcgccct ggccgatgtc cacatgccgc
    23341 gcacccatac cgaccagccg ctcgacctcg gcgcgttgat cgtcggggcg gaagtcgaag
    23401 tggatgcggt tcttgacgat tcgcctgtcg tcgacctgta gaaacaacca gttcgggccg
    23461 gatcccggcg gggccgtcag cctgacgtcg ccgtcctcgt cgacgtcttg cggccagccc
    23521 agcgcctggg accaccactg ccccaacgcc gtcgggtccg ccgcgtcgat gcagatctcg
    23581 tcgaacgtca accccatgtc gtgagctcct tcttgataac cttgcccatc gcgttgcggg
    23641 gcagcgcgtc gaccactcgc acttcgcgcg gtcgtttgtg taccgaaagc tcttgtgcta
    23701 catactcgat caacaccgaa ggggcgacgt cgccgacgac gaacgcgacg atgcgctggc
    23761 ccaggtcgtc gtcgggcagc ccgaccaccg ccgcctcggc cacccccgga tgtcccagca
    23821 gcgcggtctc cacctccccc gccccgatgc gatagccgcc ggacttgatc aggtcggtcg
    23881 actcccggcc gacgatgcgg tgcatgccgt cggcgtcgat caccgcgacg tcgccggtac
    23941 ggtaccagcc gtcgtcggtg aacgccgcag ccgtcgcatc cggccggccc aggtagccgt
    24001 cgaacatcgt cggcccgcgc acctgaaggc gtgcaacggt ttcgccgtca ttcgcgacag
    24061 tggcctcgtc gtcgtccacg agccgcgtcg tcaccccggc cagcggcagt cccacccagc
    24121 ccggacgccg ctcgccgtcg gcccgggtgc tgacggtgat cagcgactcg ctggtgccgt
    24181 accgctccac cggctggtgc ccggtgagtt cggccagccg ctcgaacacg gggacgggca
    24241 gcggcgcgct gcccgacacc aacagccgcg cgggccgcag cgcgtcggcg gccgcccggt
    24301 cggcgacgac gcgtgaccac accgtcggca caccgaagaa cagcgtgcca cctgctcccg
    24361 catacgcctc gggcgtcggc ttgccggtgt gcacgaaccg gttgccgatc cgcagcgacc
    24421 cgagcagtcc gagcaccagc ccgtgcacgt ggaacaacgg caggccgtgc accagcgtgt
    24481 cctccggggt ccactgccac gcgtcggcca gcgcgtcgag gtcggcggcg atcgcccggc
    24541 ggctcagcac cacacctttc ggcggcccgg tggtgcccga cgtgtacatg atcagcgctg
    24601 tggcgtccgg cgacggctcc ggataccggt gccacgagcg cgcgtgcagg cgcaccggga
    24661 tgtggggcat accctccgcc tcgtcgggcg cttcgccgag ccaggcctgc gcgccggagt
    24721 ccgtgagaat gtgacggcgt tcgccgtggc cgacgtcggc gggcaccggc accaccggca
    24781 cgccggcgat caggcagccg gtgacggcca g
//
LOCUS       DASWBF010000307         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_613079,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000307 DASWBF010000000
VERSION     DASWBF010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613079"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..276
                     /locus_tag="VF874_11120"
                     /pseudo
     CDS             43..276
                     /locus_tag="VF874_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558687.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
     gene            complement(326..1024)
                     /locus_tag="VF874_11125"
     CDS             complement(326..1024)
                     /locus_tag="VF874_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176596.1"
                     /translation="MEKVIVLLRDAERDDRWCSRLRGSVADEVLALGVPGLTVNVRDD
                     AVRASLMTLTTLEPPVVGIVSIWVQRCYGDQAREAIQLLSAQCRSVAAYVVTESMPLV
                     PPATELGQRTTGLANVALLRRPHDLDRETWLARWQLDHTTVAIETQATFGYTQNVVWR
                     ALTPDAPAIDGIVEELFPAEAITDLRAFFGAADDADLADRMNRMIASTSAFGANEHID
                     TVPTSRYVLRTPFA"
     gene            1416..2429
                     /locus_tag="VF874_11130"
     CDS             1416..2429
                     /locus_tag="VF874_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176597.1"
                     /translation="MLAAAGVQRVAEVRVLGAIDVGRGPIGDIAVDADGATIVATNPA
                     DASVSILDPDLMSVDAVVPVGGEPFAVAAAGGRAFVGASAPTHDLLAAIDTRAKGFLA
                     GLPVDLDVVRVAAHYDGGRLFVGGTIDDAVQLAGIDVESGRVDTVALTGAESTVDAVR
                     VSPNGRLVYVATSDSSGGTLAVVDAATARIVASVPTVSPIRDVVVRGDGRVAYVLGHD
                     PQYGGFVEMIDMTAKRAFDIAWIGGHPIQFALAADGTRMYVVYRDAVAVLCLITNEIV
                     DTLAVGAQPSCVATSPDGARLYVSDYSGRVTVFAVAARPSFDDVLDVETVALSEMREL
                     APA"
     gene            complement(2433..>3408)
                     /locus_tag="VF874_11135"
     CDS             complement(2433..>3408)
                     /locus_tag="VF874_11135"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020172348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEX9176598.1"
                     /translation="DRKIVANEVGPGLVADTRAFLDSLEPRRLAELLIGGMSTLDLPA
                     DMRTGYLALARESLGVAEYLMPPLPNTLYTRDTTCWVYGGLTLNPLYWPARHDETLLM
                     KAIYQFHPDYVGSTVWWGDPEKHWGTATIEGGDVLVPGNGVVLIGMSERTSRQAITQV
                     AIELFKNEAATEVVVAGMPKLRAAMHLDTVFTFADRDVVTTYPHIVDAIHTFTLRPSD
                     TPPGVDVVEESKPFVDVVATALGLKELRVVETGGTAYESERQQWDSGNNLVAVEPGVV
                     VAYDRNTHTNSLLRKEGVEVITIVGAELGRGRGGGHCMTCPIIRDPVG"
BASE COUNT          576 a         1101 c         1153 g          578 t
ORIGIN      
        1 ccgtagtcag acaggaacgt tcagccaaac aaggaggagt gcatggctga gaacagtgga
       61 cccgaggaag gcgtcaaagg cgtcgtcgaa ggcgccaaag gcaaggttaa gaaagccgtc
      121 ggcaccctga gcggtcgtga cgacctgacc aaagagggca aggcccagta ggacaaggcc
      181 gatgcccagc gcgatgttgc caagagagaa gccgaggcgg aagcggcacg tggtggcgcc
      241 aaggctgctg aagagcgcca gaagcaacac cagtagaagc catcccgcgc gggcccggtt
      301 caccaggacc gggcccgcct tgttgtcagg caaacggcgt gcgcaggacg taccggctgg
      361 tggggacggt gtcgatgtgc tcgttggcgc cgaacgcgga ggtgctcgcg atcatccggt
      421 tcatccggtc ggccaggtcg gcgtcgtcgg cggcgccgaa gaacgcgcgc aggtccgtga
      481 tcgcctcggc aggaaacaac tcctcgacga tcccgtcgat ggccggggcg tctggggtca
      541 gcgcgcgcca cacgacgttc tgcgtgtagc cgaatgtcgc ctgcgtctcg atggccactg
      601 tcgtgtggtc gagttgccaa cgtgccaacc aggtttcgcg gtcgaggtca tgaggacggc
      661 ggagcaacgc aacgttggcc aaacctgttg tccgctggcc taattcagtg gccggcggca
      721 ccaaaggcat cgactcggtg acgacgtacg ccgcgacgct tcggcactgg gcggaaagca
      781 gttggatcgc ctcccgggct tggtcgccgt agcaccgctg aacccagatg ctgacgatgc
      841 ctaccaccgg aggctcgagt gtggtcagcg tcatcaacga tgcccgcaca gcatcgtcgc
      901 ggacgttgac cgtcaacccc ggcacgccaa gcgccaggac ctcgtcggcc accgatcctc
      961 gcagccgcga gcaccaccgg tcgtcgcgct cggcgtcgcg gagcagcacg atcaccttct
     1021 ccatgcggat caacgtagcg ccacgctgtg aacagcacgg cacacgtcgc gtggtgtgat
     1081 ttagccctca tttgccggcc agatactaag aattcgttat aaagcaaagt tccccacgcg
     1141 acgattgctg cgttgccagc agcgcaacca cgcacgcaac agcgggttga acgttagctg
     1201 aaaatcctcc tgaacgaagg gccggaactt ttcctaagtg ctctctgaga ggtccgcagc
     1261 gcactctgcg catgtgccgc gcgccacggg ttcggcgcga catctgatgg ctagactcga
     1321 ccgagacgtg cgcctcgacg gcgcagtgaa acgaaaatca agggccggct aagcaaatgc
     1381 cggcggaggt ttgattgatt atggcgaatt ctttgatgct ggctgctgcc ggcgttcagc
     1441 gggtcgccga ggtgcgtgtg ctcggtgcga tcgacgtcgg acgaggtccg attggcgaca
     1501 tcgccgtcga tgccgatggc gccaccattg tggcgacgaa cccagccgat gccagcgtct
     1561 cgattctgga ccccgacctg atgagcgtcg acgcggtggt gcccgtcggc ggtgaaccgt
     1621 tcgcagtcgc ggcggccggc gggcgcgcgt tcgtcggggc ctctgcgccg acgcacgacc
     1681 tgctcgcggc cattgacacc cgcgccaagg gcttccttgc cggccttccg gtggacctgg
     1741 acgtcgtcag ggtcgccgcc cactacgacg gcggccggct gttcgtcggc gggacgatcg
     1801 acgacgcggt ccagctcgcg ggcatcgacg tcgaatccgg tcgcgtcgac accgtcgcgc
     1861 tgacgggcgc cgagagcacc gtcgacgccg ttcgcgtctc gccgaacggc cggctggtgt
     1921 acgtcgccac ctcggactct tctggcggga ctctcgcggt ggtcgacgcc gcgacggccc
     1981 gcatcgtggc gtcggtgccc accgtgtcgc ccattcggga cgtggtcgtg cgcggtgacg
     2041 gccgtgtcgc gtatgtgctt ggccacgacc cccagtacgg cggcttcgtc gagatgatcg
     2101 acatgaccgc gaagagggcg ttcgacatcg catggatcgg cgggcatccc attcagttcg
     2161 cactcgctgc cgacggaacc cgcatgtatg tcgtctaccg agacgccgtg gcggtgttgt
     2221 gcttgatcac caacgagatc gtcgacaccc tcgccgtcgg cgcgcagccg tcatgcgtcg
     2281 cgacgagccc cgacggtgcg cggctgtacg tctccgacta ctcgggccgg gtgaccgtct
     2341 tcgcggtcgc ggcgcggccg tcgttcgacg acgtgctgga cgtggagacc gtcgcgctgt
     2401 ccgagatgcg agagctcgcg ccggcctgac ggtcagccga cagggtcgcg gatgatcggg
     2461 caggtcatgc agtgcccgcc accccggccg cggccgagtt ctgcgccgac gatggtgatg
     2521 acctcgacac cttccttgcg cagtagcgaa ttggtgtggg tattgcggtc gtaggcgacg
     2581 acgacgccgg gttccaccgc caccaagttg ttgccgctgt cccattgttg gcgctcggat
     2641 tcgtaagcgg tgccgccggt ttcgacgacg cgcagctcct tgagccccaa ggccgtggcg
     2701 accacatcga caaacggctt ggactcctcg acgacgtcca cccccggcgg agtgtcgctg
     2761 ggacgcagcg tgaacgtgtg gatggcgtcg acgatgtggg ggtaggtcgt gacgacgtcg
     2821 cggtcggcga acgtgaacac cgtgtcgagg tgcatggctg ctcgcagctt cggcatgccc
     2881 gccaccacca cctcggtggc tgcctcgttc ttgaacaact cgatggccac ctgcgtgatc
     2941 gcctgccgtg acgtgcgctc gctcatcccg atcagcacca cgccgttgcc gggcaccagc
     3001 acgtcgccgc cctcgatcgt cgctgtcccc cagtgctttt cgggatcgcc ccaccagacc
     3061 gtcgagccga cgtagtcagg gtggaactgg tagatcgcct tcatcagtag cgtctcgtcg
     3121 tggcgggcgg gccagtacag cgggttcagc gtcagcccgc cgtagaccca gcaggtggtg
     3181 tcgcgggtgt agagggtgtt gggcagcggc ggcatgagat attcagcgac accgagggat
     3241 tcgcgtgcca gcgccaggta tccggtgcgc atgtccgcgg gcaggtcgag tgtcgacata
     3301 ccaccgatca gcagctcggc cagccgccgc ggctccagcg aatccaggaa cgcccgggtg
     3361 tcggcgacca gccccggccc gacctcgttg gcgacgatct tgcggtcc
//
LOCUS       DASWBF010000308        18660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1278431,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000308 DASWBF010000000
VERSION     DASWBF010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18660
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278431"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VF874_11140"
     CDS             complement(<1..366)
                     /locus_tag="VF874_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019730775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9176599.1"
                     /translation="MAAPVNKPRTPPYKVAGLALTAVVVVLLTLLYFQFNGTFAPKTQ
                     LTLMAPRAGLVLDSGSKVTYNGVVIGKVADVEEIDADGEPAAKVTLDVNPKYMMSIPA
                     NVEAHIRATTVFGNKYIAFT"
     gene            483..1875
                     /locus_tag="VF874_11145"
                     /pseudo
     CDS             483..1875
                     /locus_tag="VF874_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382054.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
     gene            complement(1865..3793)
                     /locus_tag="VF874_11150"
     CDS             complement(1865..3793)
                     /locus_tag="VF874_11150"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9176600.1"
                     /translation="MRNRHAGEPFTTSDADIAAALEDVSVPALLLSCIHMSGDDSLLG
                     GPLRPAGIFLNEVQGFMSEQDQAAARRLALDVIRDYRDRGCPEPAPVDAAQLKRMMDW
                     LVCTDVPDEYVPMMLEEMELDGRDSRVTVFESSPQARADFPVVVVGCGQSGLLAAIRL
                     QEAGMPYTVIEKNAGVGGTWWENSYPGARVDVGNHFYCYSFEPTDHWTEYFAQQPELQ
                     EYFEAVMHRHGITEQVRFSTEVLEAEFDDATSTWSVRIRDADGTEETLEAHAVISAVG
                     QLNRPSIPDISGAETFTGPAFHTARWDHSADLTGKDVVLLGAGASGFQVAPAIADDVR
                     SLTIFQRTAQWMFPNPNYHAKVGPGVQWALKHLPFYGRWYRFLLFWPACDTAFAAARV
                     DPDWPDQQHAVSAANDMARVMFTEWIRSQIGGDTALLEKAIPDYPATGKRTLQDNGSW
                     LRTLTKEHVELVRDGVDHIEPDAVVGSDGRRYPADVLVYATGFRVNEFISSFRVVGRD
                     GLDLQTAWGPRPAAYLGITVPNFPNFFCLYGPGTNLATGGSLIFHSECEVRYIMSCLD
                     LLIRTGAKTMEPRQDRYDDWYTRSQAEMDTLVWSQPTIKHSFYKDPNGKVHGLSPWRL
                     VDFWAWTRSVNPDDFVIR"
     gene            3916..4248
                     /locus_tag="VF874_11155"
     CDS             3916..4248
                     /locus_tag="VF874_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176601.1"
                     /translation="MGGPDNTLSPSESLDSDEVRNDDGDEVVDPPERWIEARDDESLD
                     ERLAAEEPDTSADGPPPDPATLQDGVSSSEFAVKETDVVDGVIVEDSRVSRGQIDGTP
                     EDGDSFFG"
     gene            complement(4245..4793)
                     /locus_tag="VF874_11160"
     CDS             complement(4245..4793)
                     /locus_tag="VF874_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX9176602.1"
                     /translation="MKALIITDVQNDFCEGGSLEVVGGARVARAINDHLADTHYDHVV
                     ATKDFHINPGDHFSDHPDYRSSWPRHCVVGTPGAEFHPDLDTGAIEAVFTKGQYTAAY
                     SGFEGACELGIPLTDWLRQHRVDEVVVVGIATDYCVLATAQDAARVGFSTTVLEDLTA
                     GVASETSRKALDDLRSMGVAVA"
     gene            complement(4814..5605)
                     /locus_tag="VF874_11165"
     CDS             complement(4814..5605)
                     /locus_tag="VF874_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX9176603.1"
                     /translation="MAVKVTDLLGTQLDALRYEPTAKRLRASVDSHVVADTCDGLLIW
                     EPRRLVPTYAVPAQSLSADLLPSDTGPHASQPLLDPRNPFTQHTTEGEAYDVVVESKT
                     LPSAAFRPDDPDLADYLLLDFAQFDWREEDEPIVGHPHDPFHRIDILRSSRHVRIELD
                     GQLLAESRTPVLLFETLLPVRFYLPPDDVTAHLEPSDTVTYCAYKGRASYFSLPGGRN
                     DIAWTYPEPLREAEAVRDCVCFFDEHVDVTVDGRQRTHPTTPWSD"
     gene            5696..7075
                     /locus_tag="VF874_11170"
     CDS             5696..7075
                     /locus_tag="VF874_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX9176604.1"
                     /translation="MFEEVPPEPEALRGADDDAVVAAIEEWNRLESAVSARRLDAIAE
                     LTSRRCDKEGERAQWACDGWDFAAAEVAAALGVSHKKASSQMSLSLTLRHTLPKVAAL
                     YVDGRVSYRVISAIAWHTHLVTDDAPLALIDAALAERAQQWGDLSDYKLAQAIDVWVD
                     RYDRDAVRRLRTRARSRDLYVGKKDDESDTTAVWGRLYATDAALLDRRLMELADGVCE
                     DDPRTIAQRRGDAMGVLAAGADRLACTCANPDCPAGGNDGRAANVVIHVVTEATALEA
                     KPDPQMSGEAKQPESHPPSEGRVKAPAGLLLRGGIVPTPLLAELIRSGATVREVQRPG
                     DAPEPGYRPSAKLEQFIRCRDLTCRFPGCEEPAEFCDLDHTIPYPVGPTHPSNLKYLC
                     RKHHLLKTFWMGWADQQLPDGTVLWTAPTGKTYKTLPGSRIFFPPGTPPPPNYPNHNQ
                     RRPRPPTAA"
     gene            7075..7284
                     /locus_tag="VF874_11175"
     CDS             7075..7284
                     /locus_tag="VF874_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176605.1"
                     /translation="MMPRRRRTRAADRARRIQEERALNAAAHIAQRAVNNPTEHNKPP
                     PPRDDPCDIWDIEPGTVIDDEPPPF"
     gene            complement(7349..7642)
                     /locus_tag="VF874_11180"
     CDS             complement(7349..7642)
                     /locus_tag="VF874_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9176606.1"
                     /translation="MFRTLRILTAVVATGLASAVIAVAPAAATENEYLRQLEPKYPFL
                     SRQQLLDEAYRVCQAVDSGMISSDAATMVQKDLSASVALSVDLVSTAIVQLGC"
     gene            complement(7692..8817)
                     /locus_tag="VF874_11185"
                     /pseudo
     CDS             complement(7692..8817)
                     /locus_tag="VF874_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8848..9674)
                     /locus_tag="VF874_11190"
                     /pseudo
     CDS             complement(8848..9674)
                     /locus_tag="VF874_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894717.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
     gene            complement(9753..10532)
                     /locus_tag="VF874_11195"
     CDS             complement(9753..10532)
                     /locus_tag="VF874_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9176607.1"
                     /translation="METVSVERDGHVLMIGLNRPEKRNAFTQVMLAELARAYALLESD
                     DSLRAGVLFAHGEHFTAGLDLLDVGPAIANGTDPMPEDGRDPWRLDGTWTTPVVAAAH
                     GRCLTLGIELLLAADIRIAAADTRFAQIEVLRGIYPFGGATLRFPRELGWGNAMRWIL
                     TGDEFDAAEAHRIGLVQEIAPDPDAALARAREIAHTIADHAAPLGVRATLASAHRARD
                     EGDAVAIAQLRPEVTRLFGTEDAAEGVRSFVERRRAVFSGR"
     gene            10701..10850
                     /locus_tag="VF874_11200"
     CDS             10701..10850
                     /locus_tag="VF874_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176608.1"
                     /translation="MTIIELNIAGLHLTRSAPDYHGFRRSWRRNCREWRRNVKSGRHA
                     ADPAA"
     gene            10847..11494
                     /locus_tag="VF874_11205"
     CDS             10847..11494
                     /locus_tag="VF874_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9176609.1"
                     /translation="MTDGGPATATVLKERHKRGATKRRMLVSAAEVLRERGAAGVTID
                     EVLSRSGAPRGSVYHHFPRGRSQILLEALQFAGDSMTSAIDDAATQGPMALLRGFVDL
                     WENVLQDSDFTAGCPVVAAAISASDEGAQLGGDASRIFDRWRAALTRSFVADGFGESE
                     AASLAITMLAAIEGAVILCRSLRSPQPLRDVAENLEFLVNGKQFVSRFGRPLAAR"
     gene            complement(11499..12113)
                     /locus_tag="VF874_11210"
     CDS             complement(11499..12113)
                     /locus_tag="VF874_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9176610.1"
                     /translation="MAVADVADAATDAEHPFRRRLLDGLAESIAERGYRETTIADIVR
                     HARTSKRTFYQEFDSKKECFVELLRTAIEDMIVQIEAAVDPEAFWQDQIRQAIDGYLS
                     VIESRPAVALSWIRELPALGAAVRSVQRHNIEKLTQMLTDLTSNPGFQRADIPPAPPQ
                     LSVILLGGLRELTALTVEDDKDVRNIVDAAVAASIALVAASAQA"
     gene            12221..13534
                     /locus_tag="VF874_11215"
     CDS             12221..13534
                     /locus_tag="VF874_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9176611.1"
                     /translation="MSEATVLEPRLVKLPPTVALPKAIQGVGFAMSRRWTIRQAARRY
                     GDVFTLNIPVFGNTVVVADPALVKQVFMASTDDLLNIKPNLSRLLGPGSIFALDRAPH
                     RNRRKLLTPPFHGKSIKNYEQIIEEETLREAANWPVGQELRTLEPMMRITLNVILRAV
                     FGAEGLELEYLRQLIPGWVKLGSRLATLPQPSRTYGRYSPWGRLAESHRKFDEIVDTL
                     IEKAEADPRFDERTDILALMLRSRYDDGSPMSRREISDELLTLVAAGHETTASTLAWA
                     FERLRRHPDIVAALAEEADGDDNELRQATILEVQRARTVIDFAGRHVAAPMFELGEWR
                     IPQGYSIVVSISRLHENSFADPDRFDPYRFVGNRPPTFAWVPFGGGTRRCIGAAFANT
                     EMDVVLRTVLRQFAIDSTTDPGEKVHSRGVAYTPKRGGRVVLRRR"
     gene            complement(13531..14040)
                     /gene="msrA"
                     /locus_tag="VF874_11220"
     CDS             complement(13531..14040)
                     /gene="msrA"
                     /locus_tag="VF874_11220"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9176612.1"
                     /translation="MSDKKAILAGGCFWGVQELIRRQPGVIGTRVGYTGGENANATYR
                     NHPGHAEAVEITYDPELTDYRALLEFFFQIHDPTTKNRQGNDVGTSYRSAIFYTDDEQ
                     KRVALDTIADVEASGLWPGKVVTEVTLAGDFWGAEPEHQDYLQRYPSGYTCHFVRPGW
                     KLPRRAETG"
     gene            complement(14037..14501)
                     /gene="msrB"
                     /locus_tag="VF874_11225"
     CDS             complement(14037..14501)
                     /gene="msrB"
                     /locus_tag="VF874_11225"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX9176613.1"
                     /translation="MAHKYTKNPAAVGALTPEQYEITQKNGTERPFTGEYWNNHEPGL
                     YVDVVSGEPLFASVDKFESGTGWPSFTKPVDAENVVEKRDFGHFMIRTEVRSTRGDSH
                     LGHVFPDGPADAGGLRYCINSASLRFIHRDDLEKEGYGEYVSLFETTKENRA"
     gene            14569..15087
                     /locus_tag="VF874_11230"
     CDS             14569..15087
                     /locus_tag="VF874_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9176614.1"
                     /translation="MADGAFDRVGPRASRGELSAAFQRFERTVARAAETRDWDPWVLQ
                     YTPDIEYIEHAAGTMKGRDEVRAWIWKTMSTFPGSYMTSFPTLWSVVDEPTGRIIMEL
                     DNPMADPGDGSIISATNISIITYAGDGLWCRQEDIYNPLRFVKATTKWCRKAQQLGTL
                     PDEAARWMEQYG"
     gene            15084..16130
                     /locus_tag="VF874_11235"
     CDS             15084..16130
                     /locus_tag="VF874_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9176615.1"
                     /translation="MTTSLVIGANGFLGSHVTRQLVENGADVRAMVREGANTTGIDDI
                     AVQRFSGDVFDTEVLRQAMAGCDVVYYCVVDTRAWLSDPAPLFRTNVDGTRNVLEVAR
                     SMDLRRFVFTSSYVTIGRRHGRWPSALRASAHRRATEEDQLHWRRSLGPYVLSRLQAE
                     QLVLRYAQEHGLPAVAMCVSTTYGSGDWAMTPHGAMIAGAAFGKVPFVMKGIQLEVVG
                     VDDAARAMILAAEHGRNGERYIVSEKMMTNVDVVRIAAEAARAPVPSRSVSLPVLYGM
                     AATGSLKARLRGSQELLTLRSLRLMRAESELDHSKAVRELGWQPEPVEDSIRAAARFW
                     VDLRAAMKKAKAAD"
     gene            complement(16127..17071)
                     /locus_tag="VF874_11240"
     CDS             complement(16127..17071)
                     /locus_tag="VF874_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9176616.1"
                     /translation="MRIDVHAHYWTDAYLDMLVDLGRTDTATQRGIGAGPYAELDTRL
                     RLMDRAGVDIQVLSAAPQLPYADDEDRATAAAQFVNDEYTAVVDAHRDRFLAFAATPL
                     PHIDASLAELSRALDELGMVGMTMNASVANRAIVDPDFEPIFAELNRRSTVLYLHPSG
                     NSACSPLVSEHHLTWMVGAPFEDTVAAMQLITSGTLDRYPRIRIVNSHLGGALPMLTR
                     RADDHVAWEAPDTPEPPSTAARRMWYDTVSHCHAPALRCAIDTFGADRIVLGTDFPYE
                     NDETFVRAVDYIAETASTEDTRKILETNAMQLFDIELR"
     gene            17110..17936
                     /locus_tag="VF874_11245"
                     /pseudo
     CDS             17110..17936
                     /locus_tag="VF874_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734672.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            17926..>18660
                     /locus_tag="VF874_11250"
     CDS             17926..>18660
                     /locus_tag="VF874_11250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9176617.1"
                     /translation="MPSEPRIGRVSHWYTTLPEFRAGLPGDRDADVCIVGAGFTGLWT
                     AYYLKRVDPSLRITVLEARFAGFGASGRNGGWASALVPGDRRRMAARYGRAGVIAWQR
                     ALIESVDELIDVAAREKIDAGVVKGGSLRVARNPAQHERLIAQATEDAEWSDDVSLLT
                     KTEAAQRVRVAGMLSASFTPHCARIQPAALVRGLACAVERLGVTIYEQSPVTAISTGR
                     AETLSGRVSAPVVVRATEGFTASLRGL"
BASE COUNT         3034 a         6070 c         6383 g         3173 t
ORIGIN      
        1 cgtgaatgcg atgtacttgt tgccgaacac cgtcgtggcc ctgatgtgcg cctcgacgtt
       61 cgccgggatc gacatcatgt acttcgggtt gacgtccagc gtgaccttgg ctgccggctc
      121 gccgtcggcg tcgatctcct cgacgtcggc gactttgccg atcaccaccc cgttgtacgt
      181 gaccttggat ccggaatcca ggaccagccc cgcgcgcggc gccatcaggg tcagctgcgt
      241 cttcggtgca aacgtgccgt tgaattggaa atacagcagc gtcaacagca ccacgacgac
      301 tgccgtcagc gccaacccag ccaccttgta cggcggcgtg cgaggcttgt tcaccggcgc
      361 ggccatcggg ctacacggta aggcgtgacc cgccgtcccg ggggagataa cgacaagctg
      421 agcggtgctc agccgtcgaa tgatggtgtg cttacccgca gccgcgacac aatgacgtca
      481 ccatgacttc cgacatcgcc ttcgccggcg cggccgagca ggcgcgcctg ttggccgacg
      541 gcgcaatcac tgcgccgacg ctgctcgaga cgtatctgga ccgcatcaga cggctcaacc
      601 cggtgctgcg cgcctaccgg gtggtgctgg aggagtcggc ccgcgccgag gccgcgaccg
      661 cgcagcgacg actcgatgcg ggtgagcggt tgccgctgct cggcgttccg gtcgcgatca
      721 aggacgacgt cgacgtcgcc ggcgaggtca ccacgatggg caccagcgca cacggcccgg
      781 cgaaagaaca cgacgccgag gtcgtgcgta ggctccgcga ggcgggagcg gtgatcgtcg
      841 gcaagacgca cgtgccggag ctgtgcattt ggccgttcac cgagtcgctg acgttcgggg
      901 ccacccgaaa cccgtgggac gtcggacgca cgcctggcgg cagcagcggc gggtcggggg
      961 ttgcggtggc cgcggggctt gccgggttgg cgctggggtt cggacggcgc ggggtcgatc
     1021 cggatcccgt cgacgtggtg cggcgtatac ggcatcaagc cgcagcgcga ccgggtgccg
     1081 ctggctccgc acgacgacgc gtggaacggg ctcagcgtca acggcccgat ctcgcgcact
     1141 gtcgccgacg cggcgttgtt cttggacgtc accacgtcga tgcccgcgcc cgagggcggg
     1201 ttcgtcgcgg ccgcagcgcg ccagcccggg cgactgcgaa ttgcgttgag caccaaggtt
     1261 cctccgccgc tggtcaccgt cgtcggcaag gcgcagcgcc gcgccgtcca tgacgcgggc
     1321 gtgctactgc gcgaacttgg ccacgatgtc gtcgcccgcg accccgacta cccggtgacg
     1381 gccacgttga cgaacctgct cccgcgctat ctgcgcggcg tccacgacga catggcggcg
     1441 atgccgctgc cgcagcggct ggaggcccgc acccggggga tggcccggct gggcggcctg
     1501 ttctcggatc ggcggatcgc cgcggttcgt gcggccgagg accggattcg ggcgcgggtg
     1561 ctgtcgatct tcgacgacgt cgacgtggtg atcaccccgg gcaccgcgac ggggccgtca
     1621 cgcatcggcg cgtaccagcg ccgcggtgcg gtggcgacgc tgctgctggt cggtgcgcgc
     1681 gtgccgttcc aggagatgtt caatgtcacc gggcaaccgg cagcggtcgt gccgtggggc
     1741 ttcgaccgcg acgggcttcc ggtgtcggtc cagctggtcg gccggccgtc tgacgagagc
     1801 accctgctgt cgctgagcgc tcagatcgag tcggcgcgac cgtgggcgga acgccggccg
     1861 cgcgtcagcg gatgacgaag tcgtcggggt tgacgctgcg ggtccacgcc cagaaatcga
     1921 cgagtcgcca cggactcaac ccgtgtacct tcccgttcgg atctttgtag aagctgtgct
     1981 tgatggtggg ctgtgaccag accagggtgt ccatctcggc ttgggagcgg gtgtaccagt
     2041 cgtcgtatcg atcctgccgc ggctccatgg ttttcgcgcc ggtgcggatc agcaggtcga
     2101 gacaactcat gatgtagcgt acttcgcact ccgagtggaa gatcaggctg ccgccggtgg
     2161 ccaggttggt gcccggcccg tacaggcaga agaagttcgg gaagttcggc acggtgatgc
     2221 cgaggtaggc ggcgggccgc ggaccccacg ccgtctgcag gtcgagcccg tcacgcccga
     2281 ccactcggaa cgacgagatg aactcgttga cgcggaagcc ggtggcgtag accagcacgt
     2341 cggcggggta gcggcggccg tcgctaccga cgacggcgtc gggttcgatg tggtcgacgc
     2401 cgtcgcggac gagttcgaca tgctccttgg tgagggttct cagccagctg ccgttgtcct
     2461 gcagcgtgcg cttgccggtg gcagggtagt cggggatcgc cttctcgaga agcgctgtgt
     2521 caccgccgat ttggctgcgg atccactcgg tgaacatgac ccgcgccatg tcgttggcgg
     2581 cgctgacggc atgctgttgg tccggccagt cggggtcgac gcgcgcggcg gcgaacgcgg
     2641 tgtcgcacgc aggccagaac agcaggaatc gataccaccg gccgtagaac ggcagatgct
     2701 tcaacgccca ctgcacgccg gggcccacct tggcgtggta gttcgggttg gggaacatcc
     2761 actgcgcggt ccgctggaag atggtgaggc tgcgcacgtc gtcggcgata gccggagcca
     2821 cttgaaaacc gctggcgccc gcaccgagca gaacgacgtc cttcccggtg aggtccgcgc
     2881 tgtggtccca ccgcgccgtg tgaaaggcag ggcccgtaaa cgtttccgcc ccactgatgt
     2941 ccgggatcga cggtcggttg agttgcccga cagcgctgat caccgcgtgt gcttcgagtg
     3001 tctcctcggt gccgtcggca tcacggatcc gcaccgacca cgtcgacgtc gcatcgtcga
     3061 actccgcttc taggacttcg gtggaaaacc ggacctgctc ggtgattccg tgccgatgca
     3121 tgactgcttc gaagtactct tgcagctccg gctgttgcgc gaagtactcg gtccagtggt
     3181 cggtgggctc gaagctgtag cagtagaagt ggtttccgac gtcgacacgg gcgccgggat
     3241 agctgttctc ccaccaggtt ccgccgactc cggcgttctt ctcgatcacc gtgtacggca
     3301 tgccggcctc ttgcaggcgg atcgcggcga gcagcccgga ctgtccgcag cccaccacga
     3361 cgaccggaaa gtccgcccgc gcttggggac tggactcgaa cacggtgaca cgcgagtcgc
     3421 gcccgtcgag ctccatctcc tcgagcatca tcgggacgta ttcgtcgggc acgtcggtgc
     3481 ataccagcca gtccatcatt cgcttgagct gggcggcgtc gaccggcgcc ggctcgggac
     3541 agccgcggtc gcgatagtcg cggatcacat ccaacgcgag cctgcgggca gcggcttggt
     3601 cctgttccga catgaagccc tgcacctcgt tgaggaagat cccggccggc cgcaacggac
     3661 cgccgagcag cgagtcgtca ccgctcatgt ggatgcagga cagcagcagc gcgggcaccg
     3721 agacgtcttc cagcgcggcc gcgatgtcgg cgtccgaggt ggtgaatggc tcaccagcgt
     3781 ggcgatttcg catcggcaca cctgcgcgca cggcccgaag cgtatcccag cgccgtaagc
     3841 tactgtctgt gtctactgtg ccgatactcc gggcaaacga tcgccatgcc aaccgacagc
     3901 gctgacggtc ctgacgtggg cgggccggat aacaccctga gcccctcgga gtcgctggac
     3961 tccgacgagg tccgcaatga cgacggcgac gaggtggtgg atccgcccga gcgctggatc
     4021 gaggcgcgtg acgacgaatc gctcgacgag cgcctggccg ccgaggagcc ggatacctcg
     4081 gctgacgggc cgccgcccga tccggcgacc ttgcaggacg gcgtgtcgtc gtctgagttc
     4141 gcggtgaagg agacggacgt ggtcgacggc gtgatcgtcg aagactcgcg ggtcagccgg
     4201 ggtcagatcg acgggacccc ggaggacggc gactcgttct tcggttaggc gaccgcgacg
     4261 cccatggagc gaaggtcgtc gagggccttt ctggacgtct ccgacgcgac accggcggtg
     4321 aggtcttcga gcacggtggt gctgaacccg acgcgggcgg cgtcttgtgc tgtcgcaagc
     4381 acgcagtaat cggtggcgat gccgaccacg accacttcat cgactctgtg ctgccggagc
     4441 cagtcggtga gggggatacc gagctcgcag gcgccctcga aaccgctgta ggccgccgtg
     4501 tactggcctt tggtgaagac cgcttcgatg gcacctgtgt cgaggtcggg atggaactcc
     4561 gcgccgggcg tcccgacgac gcagtgccgc ggccatgacg accgatagtc ggggtgatcg
     4621 gagaaatggt cgcccgggtt gatgtgaaag tccttggtgg ccacgacgtg gtcgtagtgg
     4681 gtatcggcca ggtggtcgtt gatcgcccgc gcgacgcgtg cgccgccgac aacttccagg
     4741 gagccgccct cacagaaatc gttctgcacg tcggtgatga tgagcgcctt catggagcta
     4801 cggtagcgcc ggttcaatct gaccacggag tcgtgggatg ggttcgctgc cggccgtcca
     4861 cggtcacgtc gacgtgctcg tcgaagaagc agacgcagtc gcgcaccgcc tccgcctccc
     4921 ggagcggttc cgggtaggtc cacgcgatgt cgttgcgccc gccgggaagt gagaagtacg
     4981 acgcacggcc cttgtaggcg cagtacgtca cggtgtcgct gggctcgagg tgggcggtga
     5041 cgtcatccgg cgggaggtag aaccgcactg gcagaagggt ttcgaacagc agcaccggtg
     5101 ttctggactc cgcgagcagt tgtccgtcga gttcgatgcg gacatggcgg ctgctgcgga
     5161 ggatgtcgat acggtggaac gggtcgtggg ggtggccgac gatcggctcg tcttcttcgc
     5221 gccagtcgaa ctgtgcgaaa tcaagcaaca ggtagtcggc caggtcgggg tcgtcggggc
     5281 ggaacgcggc ggacggcagg gtcttgcttt ctacaacgac gtcataggcc tcgccctcgg
     5341 tggtgtgttg cgtgaacgga tttctcggat cgagaagcgg ctggcttgcg tgcgggccgg
     5401 tgtcggacgg aagcaggtca gccgaaagac tctgtgcggg aacggcatac gtaggaacaa
     5461 gccggcgcgg ctcccatatc aggagtccgt cacaggtgtc agcgacaacg tgactgtcga
     5521 ccgacgcccg gaggcgcttg gctgtgggct cgtatcgcag cgcgtcgagt tgagttccga
     5581 gcaagtcggt gaccttcaca gccatgtgct cgccataacg cggcggcgct tcaatgtcaa
     5641 ggccctctgg ccaacgcggc aattgttgtc ggtgccccct ggcatactcg aaactatgtt
     5701 cgaagaggtg ccgcccgagc ccgaggcgct gcgcggcgcg gacgacgacg ccgtcgtggc
     5761 ggccatcgag gaatggaacc gcctcgagtc agcggtgagt gcgcggcgct tggatgcgat
     5821 cgccgagctg accagccggc gctgcgataa ggagggcgag cgggcgcagt gggcgtgtga
     5881 tggttgggat ttcgcggccg ccgaggtcgc ggccgccctg ggggtcagcc acaagaaggc
     5941 atcgagccag atgtcgctca gcctcacctt gcgccacacg ttgcccaagg tggcggcgct
     6001 gtatgtggac ggcagggtca gctatcgggt gatctcggcg atcgcctggc acacccacct
     6061 ggtcaccgac gacgcgccgc tggcgctgat cgacgccgcg ctggccgagc gcgcccaaca
     6121 gtggggcgac ttgtcggatt acaagctggc ccaggccatc gatgtctggg tggatcgcta
     6181 cgaccgggat gcggtgcggc ggctgcgcac ccgggcgcgc agccgcgacc tctatgtcgg
     6241 taagaaggac gacgagtccg acaccaccgc ggtgtggggc cggctgtatg ccaccgacgc
     6301 ggcgctgctg gatcgccggc tgatggagct cgccgatggc gtgtgcgagg acgatccgcg
     6361 caccatcgcc cagcgtcgcg gcgacgccat gggcgtgctg gccgccggtg ctgatcggct
     6421 ggcctgcacc tgcgccaacc ccgactgccc ggccggcggt aatgacgggc gcgccgccaa
     6481 cgtcgtcatc cacgtggtca ccgaagcaac cgccctggag gccaagcccg acccgcagat
     6541 gtccggcgag gcgaagcagc ccgaatccca cccgccgtct gagggccggg tcaaggcgcc
     6601 ggccgggctg ctgctgcgcg gcgggatcgt gccgacaccg ttgctggccg agctgatccg
     6661 ctccggcgcc acggtgcgcg aggtgcagcg tcccggcgac gcacccgagc cgggctatcg
     6721 cccctcggcc aaactcgagc agttcatccg ctgccgcgat ctgacctgcc gcttccccgg
     6781 ctgcgaggag cccgccgagt tctgcgacct cgaccatacg atcccctacc cggtcgggcc
     6841 gacacacccg tccaacctca aatacctctg cagaaaacac catctgctga aaacgttttg
     6901 gatgggctgg gctgaccaac agctgcccga tggcaccgtg ctctggaccg ccccgaccgg
     6961 caagacgtac aagaccctgc ccggcagccg aatattcttc ccgcctggaa caccaccacc
     7021 gcccaactac cccaaccaca accagcggcg accccggcca ccaaccgcgg cctgatgatg
     7081 cccagacgac gacgaacccg cgccgccgac cgcgcccgcc gaatccaaga agaacgcgca
     7141 ctcaacgccg cggcgcacat cgcccaacgc gccgtcaaca atcccaccga acacaacaaa
     7201 cccccacccc cgcgggacga tccctgtgac atctgggaca tcgagcccgg cacagttatc
     7261 gacgacgagc cgccaccgtt ctgatctcaa ccgctagctg tgccgctcga aacccgttcc
     7321 ggcagtggta gtttgtatcc tcgatgcgtc aacagccgag ttggacgatg gcggtgctga
     7381 cgaggtcgac agacagggcc accgacgcgc tcagatcttt ctgcaccatg gtggcggcat
     7441 ccgaggagat catgccgctg tcgacggctt gacacacccg gtaggcctcg tcaagcagct
     7501 gctgcctact gaggaagggg tatttgggtt ctagttgccg cagatattca ttctcggtgg
     7561 ccgcagcggg cgctaccgcg attaccgcgg acgccaaccc ggtcgccacg acggcggtca
     7621 ggatgcggag tgtacgaaac atgatcgagc acctccagga gtcggccgaa acaagcgtag
     7681 tcgcatgcgc gtcattccga cactgcgtgc agcacgacca tcgcgcggga ctcgacctgc
     7741 aacatgcccg cggcggcgat gggctttggt tccgggccgt ctgtcggtgc agtgatcgcg
     7801 gtgtcgatca ccggaagcca tgtggcaccg aattcagctg gtggcaacgt gaattggagt
     7861 gcctcgtcgt gggcgttgaa gcagagcacg aaggagtcgt cggtgacccg ctgcccgcgc
     7921 gcgtcgaggc cggcaatccc aaacccgttc agatgtccgg ctgtccctct gcgtcgcgcc
     7981 ggcgcacggg ccgcccggtg aagaaccggc ttcggcggaa caccggatgc gcggtgcgca
     8041 gcgcggacac cgtctgggtg aactccatca acgcggtgtc ggccttgtcc caatggatcc
     8101 aggtgatgtc gttgtcctgg cagaagccgt tgttgttgcc gccttgtgtg cggcccagct
     8161 cgtcgccgtg actgatcatc ggcacccctt gggacagcag cgtcgtcgcc aggaagttgc
     8221 gctgctgtcg cgcgcggagc gcgttgatcg ccgggtcgtc agtcggtccc tcgactccgc
     8281 agttccacga ccggttgtgg ctttcgccgt ccctgttgtc ctcgccattg gcgtcgttgt
     8341 gtttctcgtt gtaggacaac aggtctcgta gcgtgaagcc gtcgtgacag gtcatgaagt
     8401 tgatcgacgc gacaggtcgc cgggcggtgt gctcgtacag gtcggccgag ccggtcaatc
     8461 ttgacgcgaa ctcgtcgaga gtcgccggtt cgccgcgcca gtagtcgcgc acagtgtcgc
     8521 ggaacttgcc gttccattcc gtcccgccgc gggatcgtac gggccgtgca cccggtatcc
     8581 ataacactgt cccggctcca cttccggcag gaagccgtgc cacacgaatc cgtcgacctc
     8641 gatgagcggc acgcgcgact cggtgccgtc gtcgtcgaac aggcacagct cgacacgttc
     8701 ggcgacctcg ctgaatatcg cgaagttcgt gcttgatccg tcgtaagacg caccgagcgg
     8761 ataggcctta cccggccact gttcccagcc gcgctcgtcc tgcggagcat cggtcacgaa
     8821 gcgcctgcct gttgagcgga tctcgagtca caaccggcaa taccggctgc gcgcaaagct
     8881 atacaacccg cacgcagcga agccgagcgc cgccagaatg agcagtacct caccgaacgg
     8941 cgccgctccc agcgtcttca cggccccgtc caatccgctg gcctacgccg ggtcggcggt
     9001 gaaggtcgcg acgacgacga gtatcccggc gccggccaac acgactcctt tcgcgatata
     9061 gccggcgatg cccaccggtg tcagcaccgg gccgcccgac accgtcagat ctttcaagaa
     9121 ccgcttggtc gcacccttgt acacgaggta gcccccatcg ccacgatcgc cacgccgatg
     9181 acgacgagca ggaacttgcc ccagccgctc tgcatcagct cagcgctcag ggtggtgttg
     9241 cgcgtggtgc tggcctcccc gctgctcacc gcgaatttgg ccgccgagaa cgcgatggcc
     9301 agatgaacca ccgctaatcc gaaagacttg agccgcttgg tgattcgctt agcgcccttg
     9361 tcctgcccgg agtgttcgct ggggtgcgga ccgaccaccg tctcggcaag gcgccacagg
     9421 cgcagcgccg ccaagcccgc cgcggtcgcc cagagcacca gggcgccgcc gccgctttgg
     9481 gccagcgtgg ccagcgcacc cgactggtcg gcggtgcccc ctgatccgag cgcgatgtgc
     9541 aggatgatgt agccgacaaa caggtgcagc acgccgctca tcgcgaaccc ggcacgcgcg
     9601 ccgaactgga acgcgtcgct gcgcgtggcg cggtcgatag caccggtgaa gacctcgcgc
     9661 gtcgacgtgt tcaagtcttt gggcgtttcg atgtggcgca gttatacccg cttacctgtg
     9721 cgtcaacact tttcgtcatg acgccgtgac ggctaccggc cgctgaacac cgcccgtcgg
     9781 cgttcgacga acgaccgcac gccttcggcg gcgtcctcgg taccgaagag gcgggtgacc
     9841 tccggccgca gctgcgcgat ggccacggcg tcgccctcgt cgcgggcgcg gtgcgcagag
     9901 gccagcgtgg cgcggactcc gaggggcgcc gcgtggtccg cgatcgtgtg cgcgatctcg
     9961 cgagcccgcg ccagcgccgc gtccgggtcg ggcgcgatct cctgcaccag cccgatgcgg
    10021 tgcgcttcgg cggcgtcgaa ttcgtcaccg gtgagaatcc agcgcatcgc gttgccccag
    10081 ccgagttctc gcgggaaccg cagcgtcgcc ccgccgaacg ggtagatgcc gcgcagcacc
    10141 tcgatctgag cgaatcgggt gtcggcggct gcgatgcgga tgtcagcggc gaggagcagc
    10201 tcgatgccga gcgtcaggca gcggccgtgc gcggcggcca cgacaggtgt ggtccaggtt
    10261 ccgtccagcc gccacgggtc gcggccgtct tcaggcatcg gatccgtgcc attggcgatc
    10321 gccgggccga cgtcgagcag atccaagccc gcggtgaaat gctctccgtg cgcgaacaac
    10381 accccggcgc gaagtgaatc gtcggattcc agcagcgcgt aggcgcgggc cagttccgca
    10441 agcatcactt gcgtgaaggc gttgcgtttc tctgggcggt taaggccgat catcagcacg
    10501 tgcccgtcgc gttcgaccga aacggtttcc atggcgcctc ctgtgtgtcg gaattatgat
    10561 ccagggcata gttatggtgg tcaatgcagg cattagcggc cccaaggtaa cgatacgatc
    10621 acagtcggtt tgacgctgac tatgtcagtc cgcatagtca tagctgcggg acgtgaaccg
    10681 ccccgcgaaa ggtaaccgct atgacgatca tcgagctgaa catcgcagga ctgcacctca
    10741 cccggtctgc gcccgactac cacggcttca ggcgctcgtg gcggcgcaac tgccgggaat
    10801 ggcgccgcaa cgtgaagagt ggtcggcacg cggccgatcc cgcggcatga ccgacggcgg
    10861 tccggccaca gcgaccgtgc tcaaagagcg gcacaagcgc ggcgccacca aacgccggat
    10921 gctggtcagc gcggcggaag tgctgcgtga acggggtgcg gccggcgtca caatcgacga
    10981 agtcctcagc cgcagcggtg caccacgcgg ctctgtgtac caccatttcc cccgcggtcg
    11041 cagtcagatc cttctcgagg cgctgcagtt cgccggcgac tcgatgacct ccgcgatcga
    11101 cgacgccgcc acgcaagggc cgatggcgct gttgcgcggc ttcgtggacc tgtgggagaa
    11161 cgtcctgcag gacagtgatt tcacggctgg ctgcccggtg gtggcggcgg cgatcagcgc
    11221 cagcgacgaa ggcgcgcagc tgggcggtga cgcgtcacgc atcttcgacc ggtggcgcgc
    11281 ggcactcacc cggtcgttcg tcgccgacgg tttcggcgag agtgaggcgg cttcgctggc
    11341 cattacgatg ctcgccgcaa tcgagggagc cgtgatcctg tgccggtcgc tgcgcagccc
    11401 gcaaccgttg cgcgacgtgg cggaaaacct cgaattcctg gttaacggaa aacaattcgt
    11461 gagccgcttc ggtcggcccc tcgctgctcg gtgacgtctc aggcttgagc gctggcggcg
    11521 accagagcga tcgacgcggc cacggcggcg tcgacgatgt tccggacgtc cttgtcgtcc
    11581 tcgaccgtga gcgccgtcag ctcccgcaaa ccgcccagca gaatcaccga caactgaggc
    11641 ggcgcgggcg gaatgtccgc tcgttggaaa ccggggttgc tggtgaggtc ggtcaacatc
    11701 tgggtcaact tctcgatgtt gtgccgctgc actgaccgca cggccgcacc gagcgccggc
    11761 aactcgcgga tccagctcag cgcgaccgcc ggccgcgact cgatcacact gaggtagccg
    11821 tcgatggcct gccgaatctg gtcctgccag aacgcctctg ggtccaccgc tgcctcgatc
    11881 tggacgatca tgtcctcgat ggccgtgcgc agcagctcga cgaagcactc cttcttgctg
    11941 tcgaactcct ggtagaacgt gcgtttcgag gtgcgggcat gccggacgat gtcggcgatc
    12001 gtggtctcgc gataacctcg ttcggcgatt gactccgcga gaccgtcgag cagccggcgc
    12061 cggaacgggt gctcggcgtc cgtggcggca tcggcgacat cggccaccgc catcacgtgc
    12121 ctcctccccg gtagctcttg atcgacacgg tactaccggg taccgtacaa caagagccgg
    12181 tggtactgag cggtaccacc gatcgcgttg ggagcgaact atgagcgagg cgaccgttct
    12241 cgagccgcgt ctggtcaagc tcccgcctac agtggcgctg cccaaggcga tccagggcgt
    12301 cggctttgcg atgtcgcggc ggtggacgat ccggcaagcg gcgcgtcgat acggggacgt
    12361 gttcacgctg aacatcccgg tcttcggaaa cacggtcgtg gtggccgacc cggcgctcgt
    12421 caagcaggtg ttcatggcca gcacggacga cctgctcaac atcaagccca atctcagccg
    12481 gttgctggga ccgggatcga tcttcgcgct cgaccgagcc ccgcaccgca accggcgcaa
    12541 gctgctgacc ccgccgttcc acggcaagag catcaagaac tacgagcaga tcatcgagga
    12601 agaaactctt cgcgaagcgg cgaattggcc tgtgggacaa gagcttcgaa ctctagagcc
    12661 gatgatgcgg atcacgttga acgtgatctt gcgtgctgtc ttcggggccg aaggcctcga
    12721 actggagtac ctgcgccagc tcattcccgg ttgggtgaag ctgggttccc ggcttgccac
    12781 gttgccgcag ccgtcaagga cctacgggcg atacagccca tggggccgac tggcggaatc
    12841 gcaccgaaag ttcgacgaga tcgtcgacac actgatcgaa aaggccgagg ccgacccgcg
    12901 tttcgacgag cgcaccgaca ttctcgcgct gatgctgcgc agccggtacg acgacggttc
    12961 cccgatgtca cgtcgggaga tctccgacga gttgctgact ctggtcgccg ccggccacga
    13021 aacgacggcg tcgacgctgg cgtgggcgtt cgagcgactg cgcaggcatc ccgacatcgt
    13081 cgcagcgctg gccgaagaag ccgacggcga cgacaacgag ctacgccagg cgacgatcct
    13141 ggaggtccag cgggcgcgga ccgtcatcga cttcgcgggc cggcacgtcg cagcgccgat
    13201 gttcgaactc ggcgaatggc gcatcccgca gggctactcc atcgtcgtca gcatttcccg
    13261 gctgcacgag aactcctttg cggacccgga tcggttcgac ccgtaccggt tcgtcggcaa
    13321 tcggccgccg acgttcgcgt gggtgccgtt cggcggcggg acgcggcggt gcatcggcgc
    13381 cgcgttcgcc aacaccgaga tggacgtggt gctgcgaacg gtcttgcggc agttcgcgat
    13441 cgactcgact accgatcccg gcgagaaggt gcactcgcgt ggtgtcgcgt acaccccgaa
    13501 gcgtggcggc cgcgtcgtcc tgcgccgccg ctaacctgtt tctgcgcggc gcggcagctt
    13561 ccatcccggc cgcacgaagt ggcaggtgta cccgctcggg taccgctgca ggtagtcctg
    13621 gtgctccggc tccgcccccc agaagtcgcc cgcgagcgtg acctcggtga ccaccttgcc
    13681 cggccacagc ccggatgcct cgacgtcggc gatcgtgtcc agggcgaccc gcttctgctc
    13741 gtcgtcggtg tagaagatcg ccgagcggta gctggtgccg acgtcgttgc cctgacggtt
    13801 cttcgtggtc gggtcgtgaa tctggaagaa gaactccagc agcgcccggt agtcggtgag
    13861 ctcggggtcg taggtgatct cgacggcttc ggcgtggccc gggtggttgc ggtacgtcgc
    13921 gttcgcgttc tcaccaccgg tgtagccgac gcgcgtaccg atcactccgg gctgcctccg
    13981 gatcaactcc tgcacgcccc agaaacagcc gccggccagg attgccttct tgtcgctcac
    14041 gcgcggttct ccttcgtcgt ctcgaacagg ctgacgtact cgccataccc ttccttctcg
    14101 aggtcgtcgc ggtggatgaa tcgcaacgac gccgagttga tgcagtaccg cagaccgccg
    14161 gcatcggccg gcccgtcggg gaacacgtgg ccgaggtggc tgtctccacg cgtcgagcgc
    14221 acctcggtgc ggatcatgaa gtggccgaag tcgcgcttct cgacgacgtt ctcggcgtcg
    14281 accggcttgg tgaaactcgg ccagccggtg ccgctctcga acttgtcgac cgacgcgaac
    14341 agcggctcgc cggagacgac gtcgacgtac aaaccgggct cgtggttgtt ccagtactcg
    14401 ccggtgaacg gtcgctcggt gccgttcttc tgggtgatct cgtactgctc gggcgtcagt
    14461 gcaccgacgg ccgcagggtt cttcgtgtat ttgtgcgcca tatcgccgtt tataaccccg
    14521 tcggcgccca gcttcatccc cctgtagaac gtgttctaac tttgtcgtat ggcggacggg
    14581 gcgttcgatc gcgtgggccc acgcgcgagc aggggagagc tgtcggcggc gttccagcgg
    14641 ttcgagcgga ccgtcgcgcg ggcggccgaa acccgcgact gggatccgtg ggtgctgcag
    14701 tacacacccg atatcgaata catcgagcac gctgccggca cgatgaaggg ccgcgacgag
    14761 gtgcgcgcct ggatctggaa gacgatgtcg acgtttcccg gcagctacat gacgtcgttc
    14821 ccgacgctgt ggtcggtggt cgacgagccg accggccgga tcatcatgga gctggacaac
    14881 ccgatggccg accccgggga cggctcgatc atcagcgcca ccaacatctc gatcatcacc
    14941 tacgccggcg acgggctgtg gtgccggcag gaagacatct acaacccgct gcggttcgtc
    15001 aaggcgacga cgaagtggtg ccgcaaggcg cagcagctcg gcacgctgcc cgacgaggcg
    15061 gctcgctgga tggagcaata cggatgacga cttctctggt gatcggtgcc aacgggtttc
    15121 tcggcagcca cgtcaccagg cagctggtgg aaaacggcgc cgatgtgcgc gcgatggtcc
    15181 gtgaaggtgc gaacacgacc ggcatcgacg acattgcggt gcaacggttt tccggcgacg
    15241 tgttcgacac cgaggtgctg cgccaggcga tggcgggctg cgacgtcgtc tactactgcg
    15301 tggtggatac ccgtgcctgg ctgtccgacc cggcgccact gtttcgcacc aacgtcgacg
    15361 gcacgcgcaa cgtcctcgag gtcgctcgct cgatggactt gcggcggttt gtgtttacca
    15421 gcagctacgt gacgatcggc cgccgtcacg ggcgttggcc ttctgctctt cgcgcaagcg
    15481 ctcatcgccg cgccaccgaa gaggaccagc tgcactggcg gcggtcgctc ggcccgtacg
    15541 tgctgtcgcg gctgcaggcc gagcagctgg tgctgcgcta cgcgcaggag cacgggctgc
    15601 ccgcggtggc gatgtgcgtt tccacgacct acggcagcgg ggactgggcg atgacgccgc
    15661 acggcgcgat gatcgccggt gccgcgttcg ggaaggtgcc gttcgtgatg aaaggcatcc
    15721 agttggaggt tgtcggcgtc gacgacgccg cgcgggcgat gatcctggcg gccgagcatg
    15781 gccgcaacgg tgagcgctac atcgtctccg agaagatgat gacgaacgtg gacgtcgtgc
    15841 ggatcgcggc cgaggcggcg cgagccccgg tgccgagccg ttccgtgtcg ctgcccgtgc
    15901 tgtacgggat ggcggcgacg ggcagcctca aagcacggtt gcgcggtagc caggaactgc
    15961 tcacgctgcg atcgctgcgg ctgatgcggg ccgagtcgga actggaccac agcaaggctg
    16021 ttcgtgaact cgggtggcag ccggagccgg tcgaggattc gatccgggcg gcggcgcggt
    16081 tctgggtgga cctgcgggcg gcgatgaaga aagcgaaagc ggcggactag cgcaactcga
    16141 tgtcgaacag ctgcattgcg ttggtttcca ggatttttcg cgtgtcttca gtgctggccg
    16201 tctctgcgat gtagtccacg gcgcgcacga acgtctcgtc gttctcgtac gggaagtcgg
    16261 tgcccagcac gatccggtcg gcgccgaatg tgtctatcgc acaacgcaac gcgggcgcat
    16321 ggcagtggct gacggtgtcg taccacatcc ggcgcgccgc cgtgctgggc ggctccggag
    16381 tgtcgggtgc ctcccaggcg acgtggtcgt cggcgcggcg ggtcagcatc ggcagcgcgc
    16441 ccccgagatg actgttgacg atccggatcc gcggatagcg gtcgagcgtc ccggaggtga
    16501 tcaattgcat tgcggcgacg gtgtcttcga acggcgcacc gaccatccac gtcaagtgat
    16561 gctccgacac cagcggcgag caggcgctgt tgccgctggg gtgtagatac agcaccgtac
    16621 ttctgcggtt caactcggcg aagatcggct cgaagtcggg gtcgacgatg gcgcggttgg
    16681 ccacgctggc gttcatcgtc atcccgacca tgcccaattc gtccagcgca cgggacaatt
    16741 cggcgagtga ggcgtcgatg tgcggcagtg gtgtcgcggc gaacgcgagg aaccggtcgc
    16801 ggtgcgcgtc gacgacggcg gtgtactcgt cgttgacgaa ttgggcggct gccgtcgcgc
    16861 ggtcttcgtc gtcggcgtac ggcagttgcg gcgccgccga cagcacttga atgtcgacac
    16921 ccgcccggtc catcagccgc agccgcgtat ccaattccgc gtagggcccg gcgccgatcc
    16981 cgcgctgcgt ggcggtgtcg gttctgccga ggtccaccag catgtcgagg taggcgtcgg
    17041 tccagtagtg agcgtgcacg tcgatccgca cgttcgcaac ggtagtaccc agccgatagc
    17101 ctcggggtga tgcacggcga taccaagaag attcgcgtgg acttcaccgg ggcccccgag
    17161 acgatgctgg cgaccctgta cgggcgggcg ctcgacgccg acgctgaccg gccgatcctc
    17221 ggtgacacct acgccaagga attggtgcag cggttggact acgactggtc caagaccgcc
    17281 atcacggcca agaacgcgcc gtcggtgacg atccgcagcg cgcacttcga cgggtgggcg
    17341 cgccagttcc tggccgtgca cgaggaggcg acggtgttgc acctcggatg cgggctcgac
    17401 agccggttct tgcgactgcg tcccggaccg ggcgtcgact ggtacgacgt cgactacccc
    17461 aacgtcatcg cgctgcgcga gcagttctac ccgccgggcg cgtcataccg gttgatgtct
    17521 gcgtcggtga ccgagctcga ttggctgtcg gcggtgccgg cggatcgtcc ggtgctggtg
    17581 ctgggcgagg gcctgacgat gtatctgacc aaggaatccg ggctggcgct gttgcgtcgc
    17641 gtggtcgagc gcttcccgtc gggagaactg cagttcgacg tgttcaaccg gctcggcatc
    17701 cggacacaga tcctcaacac gccggtgcgg cgcgcgggcg cgcggatgca ctggggagtc
    17761 gacggcccga cgagatcctt cgcgacgtgc cgggtgtgcg gctgctggtg gcaatgtcgg
    17821 tgttcgacgc cgaagggttt cgtgacgtac cgcgccgcta ccgggtgatg gcgcgggtga
    17881 tgtcacttgt accggccttg aagaacatgg cgcagttcca ccgctatgcc ttctgaaccg
    17941 cgcatcgggc gcgtatcaca ttggtacacc acgcttcccg agttccgggc cgggctgccg
    18001 ggggatcgag acgccgatgt gtgtatcgtc ggcgccgggt tcaccgggct gtggacggcg
    18061 tactacctca aacgggtcga cccgtcgctg cggatcaccg tgttggaggc gcggttcgcc
    18121 ggtttcggcg cgtccggccg caacggcggc tgggcgtcgg cgctggtacc cggtgaccgc
    18181 cgcagaatgg ccgcacggta cgggcgcgcg ggcgtcatcg cgtggcagcg tgccctcatc
    18241 gagtccgtcg acgagctgat cgacgtcgcg gcgcgcgaaa agatcgacgc gggcgtcgtc
    18301 aagggcggct cactgcgggt cgcgcgtaat ccggcccagc atgaacggct catcgcccag
    18361 gcaaccgagg acgccgaatg gagtgacgat gtcagcctgc tcacgaaaac cgaagcggct
    18421 cagcgtgttc gcgtcgccgg catgctgtca gcgtcgttca cgccgcactg cgcgcgcatc
    18481 cagccggcag cgctggttcg tgggctggcc tgcgcggtcg agcggctggg agtgacgatc
    18541 tacgaacaga gccccgtcac cgcgatcagc acgggacgcg ccgaaacgct ttccggccgc
    18601 gtcagcgcac cggtcgtcgt gcgcgccacc gagggcttca ccgcgtcgtt gcgcgggttg
//
LOCUS       DASWBF010000309        16691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_549635,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000309 DASWBF010000000
VERSION     DASWBF010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16691
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549635"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /gene="rpsM"
                     /locus_tag="VF874_11255"
     CDS             <1..244
                     /gene="rpsM"
                     /locus_tag="VF874_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX9176618.1"
                     /translation="TKDLSDEQLTQLRDYIEANLQVEGDLRREVQADIRRKIEIGCYQ
                     GLRHRRGLPVRGQRTKTNARTRKGPKRTIAGKKKAR"
     gene            248..670
                     /gene="rpsK"
                     /locus_tag="VF874_11260"
     CDS             248..670
                     /gene="rpsK"
                     /locus_tag="VF874_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX9176619.1"
                     /translation="MPPAKKGTAAASTKKGQKGRRREKKNVPHGAAHIKSTFNNTIVT
                     ITDPQGNVIAWASSGHVGFKGSRKSTPFAAQLAAENAARKAQEHGVKKVDVFVKGPGS
                     GRETAIRSLQAAGLEVGAIADVTPQPHNGCRPPKRRRV"
     gene            684..1289
                     /gene="rpsD"
                     /locus_tag="VF874_11265"
     CDS             684..1289
                     /gene="rpsD"
                     /locus_tag="VF874_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX9176620.1"
                     /translation="MARYTGPMTRKSRRLRVDLVGGDAAFEKRPYPPGQHGRARIKES
                     EYLLQLQEKQKARFTYGVQEKQFRRYYEEANRASGKTGEALLQILETRLDNVVYRAGL
                     ARTRRMARQLVSHGHFTVNGVKVDVPSYRVAQYDIIDARDTSVNTVPFQIARETAGDR
                     PIPGWLQVVGERQRILVHQLPERAQIDVPLTEQLIVEFYSK"
     gene            1367..2422
                     /locus_tag="VF874_11270"
     CDS             1367..2422
                     /locus_tag="VF874_11270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX9176621.1"
                     /translation="MLISQRPTLSEEVVADDRSRFIIEPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVTSIRIDGVLHEFTTVPGVKEDVTDIILNLKGLVVSSDEDEPVTMYLRKQGP
                     GAVTAGDIVPPAGVTVHNTDMHIATLNDKGKLEVELVVERGRGYVPAVQNKASGAEIG
                     RIPVDSIYSPVLKVTYKVEATRVEQRTDFDKLILDVESKNSISPRDALASAGKTLVEL
                     FGLARELNVEAEGIEIGPSPAEADHIASFALPIDDLDLTVRSYNCLKREGIHTVGELV
                     ARTESDLLDIRNFGQKSIDEVKVKLHQLGLSLKDSPAGFDPSEVAGYDAATGTWHGDS
                     AAYDFDNEQDYAETEQL"
     gene            2450..3148
                     /gene="rplQ"
                     /locus_tag="VF874_11275"
     CDS             2450..3148
                     /gene="rplQ"
                     /locus_tag="VF874_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX9176622.1"
                     /translation="MPKPTKGPRLGGSSSHQKAMLANLATSLFEHGRIKTTETKARAL
                     RPYAEKLITHAKKGTLHNRREVLKKIRDKDVVHTLFAEIGPFFADRNGGYTRIIKVEP
                     RQGDNAPMAVIELVREKTVTSEANRARRSRSAGDIGSSSAASQAAKKAAPVAAAAAPQ
                     AAVEPEATVAPPAEALAEEPTTQPEETVDEVKAEDEAIEEAQAAEAEAEETGDSDERL
                     REEPKAEGDDADKS"
     gene            3153..4025
                     /gene="truA"
                     /locus_tag="VF874_11280"
     CDS             3153..4025
                     /gene="truA"
                     /locus_tag="VF874_11280"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX9176623.1"
                     /translation="MRDEPAIASDGGFVRLRLDISYDGTDFAGWAVQGELRTVAGVVD
                     DALSTVFRTPVRLFAAGRTDTGVHATGQVAHVDVPADALRHARPRKMRREDPEFLPLV
                     RRLARFLPDDVRVLEIRRAPEGFDARFSALRRHYVYRLSTAPYGVEPQLSRYVTAWPR
                     PLDVDAMAAASKHLLGLHDFAGFCRQRPGATTIRDLQRLDWGRDGDLVTAHVSADAFC
                     WSMVRSLVGALLAVGDGRRDAAWCAGLLNAARRSSAFAAAPARGLTLAGVDYPPDDQL
                     AARIAVTRDLRTVD"
     gene            complement(4022..4645)
                     /locus_tag="VF874_11285"
     CDS             complement(4022..4645)
                     /locus_tag="VF874_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEX9176624.1"
                     /translation="MFPSAVLPSGTPGSAPTAAAADCPAVEVVFARGRLESPGPGILG
                     NAFISALRSRVNQNIGVYAVKYPADSEVDVGANDMSQHIQYMVNNCPDTRLVLGGYSL
                     GAAVTDVVLAVPFDVFGFKSPLPAGADAHIAAVALFGNGVAWVGPITNFNPLYRERTI
                     ELCHGADPICNPADPNTWQDNWPAHLANAYINAGMANQAADFVAGRL"
     gene            complement(4772..5422)
                     /locus_tag="VF874_11290"
     CDS             complement(4772..5422)
                     /locus_tag="VF874_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEX9176625.1"
                     /translation="MCGVSIRRVFAAALVAAGGFAVLPGAIPTVSAQPCPDIEVIFAR
                     GTREPAGIGQVGQAFVDALQEQIGNRSVAVYGVNYPASYDFLAAGDGAADASSRVISM
                     SQTCPSTRLVLGGYSQGAAVVDMLADVPILGTNLGTPLPPTVADNVAAIAVFGNPSTK
                     LGKPVSAASPLFASRAIDLCKDGDPVCSPGRNPFAHSDYVNAGLPGQAANFVAGLV"
     gene            complement(5425..6417)
                     /locus_tag="VF874_11295"
     CDS             complement(5425..6417)
                     /locus_tag="VF874_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9176626.1"
                     /translation="MDAKSTFRLGIAFAVGSALTFGMSGPFAKALMAAGWTPNAAVTA
                     RLAGGALVMALAATLIHRSWLREAVQHGKTVLVYGLIPIAGAQLCYYNAVAHLSVGVA
                     LLLEYLAPVLVVGWLWATTRRRPGTLTLAGAALALAGTVVVLDVFSGTQVDAVGVAWA
                     LGAAICAACYFLLSDRVAADGSGLHSVTLAASGLIVGAAAVAALGMTGVLPLTFTTND
                     TVVAGHTTSFVVPVVVLGVVSTAIAYTLGISGVARLRPSYASLMGLGEVLCAVVAAWV
                     LVGEAITPAQAVGGAVVLLGLTLAGRDDTQRAAVWPDAPPAEVVDQLFTTGNHV"
     gene            6494..7042
                     /locus_tag="VF874_11300"
     CDS             6494..7042
                     /locus_tag="VF874_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX9176627.1"
                     /translation="MLFSHDTELTLRAASALINSDRVDGEQLGSQSALDEYLNSYGWT
                     GRRDHDQAELAAVHELRARLGRIWECADDEERAVEQVNAMLADTHAAPWLTRHPEMPE
                     WHLHLASVRDPLAQRMGAEMAMALADLIRAGELRRLKICAAPDCDAVVMDLSRNRSRI
                     FCDTGNCANRQHVAAYRERRQR"
     gene            7112..8512
                     /gene="eccB"
                     /locus_tag="VF874_11305"
     CDS             7112..8512
                     /gene="eccB"
                     /locus_tag="VF874_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017206775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HEX9176628.1"
                     /translation="MAREPTTRLAISGHRFLLRRAEHALVRRDVRMVDEPMRAQARSL
                     VTGCALAVVAVAGCAILAFIRPPDALGDAPIVMVRESGALYVRIGDTLHPALNLASAR
                     LIAGTAASPEIIKESELRNAKRGALLGIPGAPALLPEPLGADESQWTACDDANGTTVI
                     AGAATGAQALANGRTTLVRSRSDAVTYLLHDGYRARVSVAEPAAVKALRLEGIEPADV
                     SSAVLNAIPEAPSITAPVIPAAGQRGPSSLGGIPVGSVVRVDRADADELYVVLAGGVQ
                     RVGHVGADIVRFSDSQGARDIVTVAADAIGATPTVETLPITTFPDMVAAPTRDDVVCA
                     QWLPGDTTEGRSTVLTGSAVPLATGQAPVGLAQADGLGPNADAVFVPPGRSVYAESTH
                     RYLIADTGVRFGVPDAESAVALGLPPRALPAPWPILKLLADGPELSRSAALVAHDGVA
                     PDPRAIALPGTAVAPR"
     gene            complement(8438..9835)
                     /gene="mycP"
                     /locus_tag="VF874_11310"
     CDS             complement(8438..9835)
                     /gene="mycP"
                     /locus_tag="VF874_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease
                     mycosin"
                     /protein_id="HEX9176629.1"
                     /translation="MSTMLRVFVAAALAVGLPGLAPLPAASAERPPAIDDSLLPPPAP
                     PKPHTPTEKQSDCTTAIFDGQLNPTATQLTPFDLPALWALTTGDGQKVAVIDTGVSTH
                     PRLPRLIPGGDYVSTGDGREDCDAHGTLVAGIIAATTESDGFAGIAPGVSVITIRQSS
                     VKYGPVSGRSFGVGDVDTLAMAVRTAADLGATVINISSVACTAGAALRDRALGAAVAY
                     AVDAKDAVVVVAAGNVGPGGNCPQQNPAPDPARRPEPDWETADVAVSPGWYDDYVLTV
                     GSVSTDGSPSTFSLAGPWVDVAAPGEAVVSLNPDHTGVANAYRSGTGSAIAGTSYAAP
                     VVSGLAALVRARFPDLSARQVMQRIEATAHRPPDGWDPYVGNGVVDPLAALTDGRPSA
                     PPSWAQRPVAPSAPPPPRETSGRSTAFTGVAGCAAFLAAALALISPANRLARRHRGAG
                     QRDSARVGRHTVVRD"
     gene            complement(9835..11196)
                     /gene="eccD"
                     /locus_tag="VF874_11315"
     CDS             complement(9835..11196)
                     /gene="eccD"
                     /locus_tag="VF874_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HEX9176630.1"
                     /translation="MARTVTSDICRIGVQFESGRLDFVLPADFTVTEVIPPIVDHVAA
                     SSGAAGRRRWDEIPSDWQLSRTDGRPLAPRASLRDNGVRDGDVLWLGRVSAAVAPPPA
                     DDAIAEIASSLEKIPRWTPTLTRVAVSVGTLCCTGLIAYALLRAPSSDSPVIAGLLSA
                     VALTAAVAYGRMYRQPLTAVTLGACAAASATVAGYLVVPGGATAPKLMLASAVSATVS
                     VLASRYIGRGTAVFSGLASFGFSSAAAAWVHLVAPVDVAQTGAVLAAAAAGLLVLTPR
                     LAISTGRVPIPRLPGAPVRDDGAALESIAEHASHAHAIITGLVCGFSAAAALGASLAV
                     HGGAFCGVAFAAAIGVMLALRAGTHVDVVQVAALLVSGASCFAAVFIWSVGKWAQHAH
                     WIALGAAGIAAASTVCSSAVMTPSPLIRRCVDLTEYAALGLVIPLACWVCGVFAAVRG
                     LSF"
     gene            11346..15077
                     /gene="eccCa"
                     /locus_tag="VF874_11320"
     CDS             11346..15077
                     /gene="eccCa"
                     /locus_tag="VF874_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCa"
                     /protein_id="HEX9176631.1"
                     /translation="MPDWHSGPRMAAPAIPEGEVVIESPNEPPRALPASPVVRMLPVV
                     MVVAMAGMMVLFVSSGAAAARNPSSLLFPAMMVVSSIGMIAHGRGAGARAAELDEGRK
                     DYLRYLGRVRDEAFESARCQRVSLLWQHPDPAALWTLAGTRRMWERGRADSDFCHVRV
                     GTGRQRSAAPPTAPNLGPVDQLEPVSAMALRELIRAHSVVDEVPIALALTRFGAVAVD
                     GDGARDLMRAMVCQLAVLHGPALLAIVAVVDDAAAAEWDWLKWLPHRGDSAAHTVVVV
                     DGGAVSGVAPFAERGDVTILERGRRCQGEGSRPCLRLDVCADELAVLGDVREVVARPD
                     AMAVSEAELCARRLAPYRSAADRLPTSAPGADWAQATGIGDPWALQPETIWRQRKPHN
                     RLRVAIGVAADGSAVELDLKEAAENGMGPHGLCVGATGSGKSEFLRTLALGLIATHDP
                     EALNLVLIDFKGGATFLGLERARHVSAVITNLSDEAHLVARMKDALAGEMNRRQELLR
                     TAGRFASVTDYEWAREAGAPLRPLPTLLVIVDEFSELLTQHPDFAEMFVAIGRLGRSL
                     RVHLLLATQRLDEGRLRGLESHLSYRVCLKTFSANESRAVIGRPDAFQLPATPGAAFL
                     KVGSDEPVAFQTAYVSGPCVVPATPTRPPTTPTVRLFTATPIATPAAPERAHKHAAGT
                     VLDTVLDRLAGHGTPAHQVWLPPLTASPTLDELLRTGHGVLEVPIGFVDVPFDQRRDP
                     LVVELAGAAGNVAIVGAPQSGKSTAVRTLVTAIAATHAPDLVQMYCLDFGGGALSALG
                     ALPHVGAVAGRADPDLVRRTVAELSALLRAREAAFRKLGIDSMADYRRRRAAGDPSTA
                     DDPFGDAFLIVDGWASLRDDFDALELPITALATRGLSYGVHVVLTASRWAEIRPGLKD
                     QVGTRIELRLGDAGDSELNRRAAQHVPYGRPGHGITRDGMHMLIGLPRLDGKHEATGL
                     SAAIGDAAAMIRAQFGDCAAPSVRLLPDRVDHTALIGGQPPTQMIIGVDEDELAAVTV
                     DFAENQHILILGDVECGKTAALRLVCAELVRTRSPEQAQLMVVDYRRSLLGVVESDHL
                     AGYAISGAVLTAQLPAFVDRLRARIPGPDVDQRQLRTRSWWSGPNVYLIVDDYDLVST
                     DTGNPLAPFIELLPHAKDLGLHVVVARRAGGARRALFEPLLARLRELGCLGVMMSGSP
                     DEGVLLGAARAGPLPPGRARLITRAGERVVQLGWIPPCM"
     gene            15074..16279
                     /locus_tag="VF874_11325"
     CDS             15074..16279
                     /locus_tag="VF874_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated protein"
                     /protein_id="HEX9176632.1"
                     /translation="MSAVVVGPCGVRASGVLATEQAGLAAAALDAIDDELALVDERPV
                     AVGGLWREVFAALLDGGKAVLVCPSWWSDRRIATVSDAAHAVVADVEVARRSAMLVSA
                     VARQSAVVVEIAGDFVALSRPPADRPVKIVSRDARPADVADEVVRWVASAAVVDRAAG
                     VGGAVELGAMIADRLRASGTPVTVVDDERLLRAAQKFAADDPTVPCEAIRPRRPRVWP
                     WVGAAAAAGVTITGIALASGPSPTPPTTLLVEGRVVVEVPVTWTARRITGGPGSARLQ
                     VASPKDPHAAVHVTQSLVPSGETLQRTADTLRRAMRDEPPGVFVDFNPADRRGDREAV
                     TYREIRDGHDIGWTVLLDGQVRISVGCQSARGAEESVRAVCEVAVASARAVAESVGTV
                     APQRQSNNT"
     gene            16300..16623
                     /locus_tag="VF874_11330"
     CDS             16300..16623
                     /locus_tag="VF874_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEX9176633.1"
                     /translation="MTSPGAGSLTTDFDLMRDVAGKIDARNDEIRTMLQGFIGRMTSV
                     PPSVWGGNAATAFKGVVDRWNSESTKLQQALQGIAETIRANEQSLREAADTHSAQIGA
                     VAGNL"
BASE COUNT         2783 a         5594 c         5828 g         2486 t
ORIGIN      
        1 caccaaggat ctctccgacg agcagctgac ccagctgcgc gactacatcg aagccaacct
       61 gcaagtggag ggcgatctgc gccgcgaggt gcaggccgac atccgccgca agatcgagat
      121 cggctgctac cagggcctgc ggcaccgtcg cggccttccg gtgcgcggcc agcggaccaa
      181 gaccaacgcg cgcacccgca agggccccaa gcgcaccatc gccggcaaga agaaggccag
      241 ataaccgatg ccaccagcta aaaagggcac cgccgctgca tccactaaga aggggcagaa
      301 gggccgccgc cgggaaaaga agaacgttcc gcacggcgcc gcccatatca agagcacgtt
      361 caacaacacg atcgtcacca tcaccgaccc gcagggcaac gtgatcgcgt gggcgtcgtc
      421 cggccacgtc ggcttcaagg gctcgcgcaa gtcgaccccg ttcgccgcgc agctcgccgc
      481 tgagaacgcc gcccgcaagg cgcaggagca cggcgtcaag aaggtcgacg tgttcgtcaa
      541 aggcccgggc tcgggccgcg agaccgcgat ccgctcgctg caggccgccg gcctggaggt
      601 cggtgcgatc gccgatgtca ccccgcagcc gcacaacggt tgccgtccgc cgaagcgacg
      661 ccgggtctag ggaggaagaa ctgatggctc gatacaccgg cccgatgacc cgcaagtcgc
      721 ggcggctgcg cgtcgacctc gtcggcggag atgcggcttt cgagaagcgc ccctacccgc
      781 ccggccagca cggccgtgcg cggatcaagg agagcgagta cctgctgcag cttcaggaga
      841 agcagaaggc acgcttcacc tacggcgtgc aggagaagca gttccgccgg tattacgaag
      901 aggcgaaccg ggcttccggc aagaccggtg aagctctgct tcaaatcctg gagacgcgcc
      961 tggacaacgt cgtgtaccgc gccgggctgg cgcgcacgcg tcggatggcc cgccagctcg
     1021 tcagccacgg tcacttcacg gtcaacggcg tgaaggtgga cgttcccagc taccgggtcg
     1081 cgcagtacga catcatcgac gcgcgggaca cgtcggtgaa caccgtgccg ttccagattg
     1141 cgcgggagac cgcgggcgat cggccgattc ccggctggct gcaggtagtc ggggagcggc
     1201 agcgcatcct ggtgcaccag ctgccggagc gggcacagat cgacgtgccg ctcaccgagc
     1261 agctcatcgt cgagttctac tcgaagtaaa gacttctcgg ccgtccggat ggcggtcggg
     1321 tcacgcggca tcaaatagtg ggtgccgtga aggaggaaag aaacacatgc tgatctctca
     1381 gcgacccaca ctgagcgaag aggtcgttgc cgacgaccgg tcgcggttca tcatcgagcc
     1441 gctggagccc ggattcggct atacccttgg caattcgctg cgccgcaccc tgctgtcgtc
     1501 gattcccggc gccgcggtca ccagcattcg catcgacggc gtactgcacg agttcaccac
     1561 ggtgcccggc gtcaaggaag acgtcaccga catcatcctc aacctcaagg gtctggtggt
     1621 gtcgtcggac gaagacgagc ccgtgacgat gtacctgcgc aagcagggcc cgggtgcggt
     1681 caccgccggt gacatcgtgc cgcccgcggg cgtgacggtg cacaacaccg acatgcacat
     1741 cgcgacgctc aacgacaagg gcaagctaga ggtggagctc gtcgtcgagc gcggccgcgg
     1801 ctacgtgccg gccgtgcaaa acaaggcgtc gggtgccgag atcggccgca tcccggtcga
     1861 ttccatctac tcgccggtgc tcaaggtcac ctacaaggtc gaggccaccc gtgtcgagca
     1921 gcgcaccgac ttcgacaagc tgatcctcga cgtcgagtcc aagaactcga tcagcccccg
     1981 cgacgcgctg gcgtcggcgg gcaagacgct ggtcgaattg ttcggtctgg cacgggaact
     2041 caacgtcgag gccgagggca tcgagatcgg cccgtcgccg gccgaggccg accacatcgc
     2101 gtcgttcgcg ctgccgatcg acgacctgga tctgacggtc cggtcgtaca actgcctcaa
     2161 gcgcgagggc atccacaccg tcggcgaact ggtggcccgc acggagtcgg acctgctgga
     2221 catccgcaac ttcggtcaga agtcgatcga cgaggtgaag gtgaagctgc accagctcgg
     2281 cctgtcgctc aaggacagcc cggccggctt cgacccgtcc gaggtcgccg ggtacgacgc
     2341 ggcaaccggc acgtggcacg gcgactcggc ggcgtacgac ttcgacaacg agcaggatta
     2401 cgccgaaacc gaacagctgt aagttagttc tgcacaagga gattcagcaa tgcccaagcc
     2461 caccaagggc cctcgcctcg gcgggtcgtc ctcgcaccag aaggcgatgc tggccaacct
     2521 ggccacgtcg ctgttcgagc acggccggat caaaaccacc gagaccaagg cccgggcgct
     2581 gcggccgtac gccgagaagc tgatcaccca cgcgaagaag ggcacgctgc acaaccggcg
     2641 tgaggtgctc aagaagatcc gcgacaagga tgtcgtgcat accctgttcg cggagatcgg
     2701 gccattcttc gccgaccgca acggcggcta cacccgcatc atcaaggtcg agccgcgcca
     2761 gggcgacaac gcgccgatgg cggtgatcga actggtgcgg gagaagacgg tcacgtcgga
     2821 ggcgaaccgg gctcgccgga gccgctccgc cggcgacatc ggatcgagtt cggcggcctc
     2881 gcaggcggcc aagaaggcag ccccggtggc tgcggcggcc gcgccgcaag cggcggtcga
     2941 gccggaggcg acggtcgctc cccccgctga ggccttggcc gaggagccga cgacacagcc
     3001 tgaggagacg gtcgacgagg tgaaggccga ggacgaggcc atcgaggagg ctcaggccgc
     3061 cgaggccgag gctgaagaga ctggcgacag cgatgagcgc ttgcgcgaag agccgaaggc
     3121 ggagggcgac gacgccgaca agtcctagca ctatgcgcga cgagcccgcc atcgcttctg
     3181 atggcgggtt cgttcgtctt cgcctcgaca tctcctacga cggaacggat ttcgcgggat
     3241 gggccgtaca gggcgagcta cgcaccgtcg cgggtgtggt cgacgacgcg ctgtcgaccg
     3301 tgttccgcac accggtgcgg ttgttcgcgg cgggtcgcac cgacaccgga gtgcatgcga
     3361 ccggccaggt cgcacacgtc gacgtgccgg ccgatgcact cagacacgcc cggccgcgca
     3421 aaatgcgacg cgaggatccg gaattcctgc cgctcgtgcg gcggctcgca cggttcctac
     3481 ccgatgacgt ccgggttctt gaaatccgcc gcgcaccaga gggattcgac gcacggtttt
     3541 ccgcgctgcg tcgccattac gtgtaccggt tgtcgacggc gccgtacggc gtcgagccgc
     3601 agctatcgcg ttacgtgacg gcatggccgc gccccctgga cgtcgacgcg atggcggcgg
     3661 cgtcgaaaca ccttctcgga cttcatgatt tcgccggctt ctgccgccag cgccccggcg
     3721 ccacgaccat ccgcgatctg cagcgtctgg attggggccg cgacggcgac ctggtcaccg
     3781 cgcacgtcag cgccgacgcg ttctgctggt cgatggtgcg atctttggtg ggtgcattgt
     3841 tggcggtcgg tgacggccga cgggacgcgg catggtgcgc cggcctgttg aatgcggccc
     3901 gccggtcgag tgcgttcgcg gccgcgcctg cgcgcggact caccctcgcg ggtgtcgact
     3961 atccgccaga cgaccagctg gcggcccgta tcgccgtcac gcgggatctc cgcacagtcg
     4021 actagagccg gccagcgacg aagtcggcgg cctggttcgc catgcccgcg ttgatgtagg
     4081 cgttggcgag gtgggcgggc cagttgtctt gccaggtgtt cgggtcggcc gggttgcaga
     4141 tcggatcggc gccgtgacac aactcgatgg tccgctccct gtacagaggg ttgaagttcg
     4201 tgatgggtcc cacccatgcc actccgttgc cgaacagggc gaccgcggcg atgtgcgcat
     4261 cggcgccggc tggcagcggg ctcttgaaac cgaacacgtc gaacggaacc gcgagcacca
     4321 cgtcggtgac cgccgcgccg agcgagtagc cgccgagcac cagccgggtg tcagggcagt
     4381 tgttcaccat gtactggatg tgctggctca tgtcgttggc accgacgtcc acctcggaat
     4441 cggcggggta cttcaccgcg tagacaccga tgttctggtt caccctggag cgcaaggcgc
     4501 tgatgaacgc gttgccgagg atgcctggcc ccggtgactc caaacggccg cgggcgaaga
     4561 ccacttccac ggcggggcag tcggcggcgg cggccgtcgg cgccgagccg ggcgtacccg
     4621 acggcagcac agcggacggg aacatcagca cgcccgccgt caataccgct gcgacaacga
     4681 cgatggccca ttgacgtgga tgtaaaacgc tcaccaggcg atggtagcgg cgatcgcggg
     4741 tcgcggtcgc ggcttgaccg atcagcgacc gctagacgag gccggccacg aaattcgcgg
     4801 cctggccggg cagaccggcg ttcacgtagt cgctgtgagc gaatggattc cggccaggag
     4861 agcaaacggg gtcgccgtcc ttgcacaagt caatcgccct gctggcaaac agagggctcg
     4921 cggccgaaac gggctttccc aacttcgtcg acgggttgcc gaataccgcg atcgcggcga
     4981 cgttgtcggc aaccgtcggc ggaagcggcg taccgagatt ggtacccaga atcggaacat
     5041 ccgccagcat gtccaccacg gccgcgccct gggagtatcc gccgagcacg agcctggtcg
     5101 acgggcacgt ctgcgacatg ctgatcacgc gagaactggc gtcggctgca ccgtcgccgg
     5161 ctgccaggaa gtcatagctc gcggggtagt tcaccccgta cacggccacg ctgcggttgc
     5221 cgatctgctc ttgcagtgcg tcgacgaagg cctggccgac ttggccgatg cccgccggtt
     5281 cccttgtgcc acgggcgaat atcacctcga tgtcgggaca cggctgcgcg gacacggtgg
     5341 ggatggcgcc cggcagcacc gcaaacccgc cagccgccac gagggcggcg gcaaagacac
     5401 gtcgtatgct cacgccgcac atcttcacac atggttgccg gtggtgaaca actggtcgac
     5461 gacctcggca ggcggcgcgt cgggccagac ggcggcgcgc tgggtgtcgt cccggcccgc
     5521 cagcgtcaga cccagcaaca ccaccgcgcc gccgacggcc tgcgccggtg tgatcgcctc
     5581 gccgacgagc acccaggcgg caacgaccgc gcacaacacc tcacccaggc ccatcaacga
     5641 cgcgtagctg ggccgcagcc tcgccacccc gctgatgccg agcgtgtagg cgattgcggt
     5701 gctgacgaca ccgagcacga cgaccggcac gacgaacgag gtggtgtggc cggcgacgac
     5761 ggtgtcgttg gtggtgaacg tcagcggcag cacgccagtc atgcccagcg ccgccacggc
     5821 cgcggccccg acgatcagcc cactggccgc gagcgtcacc gagtgcaacc cgctgccgtc
     5881 tgcggccacg cggtcggaca acaggaagta gcaggccgcg caaatcgcgg cgccgagcgc
     5941 ccaggccacc ccgacggcgt cgacctgggt cccggagaag acgtcgagca ccacgacggt
     6001 gccggcgagt gccagcgctg ccccggcgag cgtcagcgtg cccggccgac gccgtgtcgt
     6061 cgcccacagc cagccgacca cgagaacggg cgcgaggtac tccagcagca gcgcgacgcc
     6121 cacggacaga tgcgcgaccg cgttgtagta gcacagctgc gccccggcga tcgggatcag
     6181 gccgtagacg agcacggtct tgccgtgctg gacggcttcg cgcagccagc tgcggtggat
     6241 cagcgtggct gccagcgcca tcaccagcgc gccgcccgcc aagcgggcgg tcactgcggc
     6301 gttgggcgtc cagccggcgg ccatcagcgc tttggcgaag ggacccgaca tgccgaacgt
     6361 cagcgccgac ccgaccgcga acgcgatgcc gagccggaag gtgcttttcg cgtccatcga
     6421 cagccctcca cgccggtaat gagtaaaatc tgctttggtg ctgacgctac aaggcggtga
     6481 ggtcaggagt caaatgcttt tcagtcatga caccgaactc acactgcgtg ccgcgagtgc
     6541 gctgatcaac agcgatcgcg tcgacggcga acaactcggc tcccagtcgg cgctcgatga
     6601 gtacctgaac tcgtacgggt ggaccggacg ccgggaccac gaccaggccg agctcgcggc
     6661 cgtgcacgag ttgcgggcac ggctgggccg aatctgggag tgcgccgacg acgaggagcg
     6721 cgcggtcgag caggtgaacg cgatgctcgc cgacacccac gcggcgccgt ggctgacgcg
     6781 gcacccggag atgcccgagt ggcatctgca tctcgcgtcg gtgcgcgacc cgctggcgca
     6841 gcggatgggc gccgagatgg cgatggcgct ggccgacctc atccgggccg gcgagctgcg
     6901 ccgactcaaa atctgcgcgg cgccggattg cgacgcggtg gtgatggacc tgtcgcgcaa
     6961 ccggtcgcgg atcttttgtg acaccggaaa ctgcgccaat cgccagcatg ttgcggccta
     7021 ccgcgagcgg cggcagcgat gagcagaatt cccgacactg cgcctgctgg agccgagtta
     7081 ggttagagct gagggtttgc ggggggagca catggctcgg gaaccgacaa ctcggcttgc
     7141 gatcagcgga caccgatttc tgttgcggcg cgcggagcat gcgctggtgc gcagggacgt
     7201 ccgcatggtc gacgaaccga tgcgcgcgca ggcgcggtcg ctggtgaccg ggtgcgcact
     7261 ggccgtcgtc gccgtggcgg gctgcgcgat cctcgccttc atccgccccc cggacgccct
     7321 cggggacgca ccgatcgtga tggtccgaga gtctggcgcg ctgtacgtcc gcatcggtga
     7381 caccctgcat cccgcgttga acctcgcctc ggcacgcctg atagcgggga cggccgcgag
     7441 cccggagatc atcaaggaat cggaactgcg caacgcgaaa cgcggtgcac tgctgggcat
     7501 ccccggtgcg ccggcgctct tgcccgagcc gttgggtgcc gacgaatcgc agtggacggc
     7561 gtgcgacgac gccaatggca cgaccgtgat cgcaggagct gcaaccggcg cgcaggcgct
     7621 cgcgaacggc cgaacgacac tggtccggtc acgctcggac gcggtgacgt atctgctcca
     7681 cgacggctac cgtgcgcggg tgagcgttgc cgagccggcg gccgtgaagg ccttgcgcct
     7741 cgagggaatt gaaccggcgg acgtgtcgtc ggcggtgctg aatgcgattc ccgaggcgcc
     7801 atcgatcacc gcaccggtca ttccggcggc cggacagcga ggcccgtcgt cgctgggcgg
     7861 catacccgtc ggttccgtgg tgcgggtgga tcgcgccgac gccgacgagc tgtatgtggt
     7921 gctcgccggc ggtgtgcagc gggtcgggca tgtgggcgcc gacatcgtcc ggttcagcga
     7981 ctcgcagggc gcccgcgaca tcgtcacggt cgccgctgac gcaatcggcg ccacgccaac
     8041 tgtcgaaaca ttgcctatca caacgtttcc tgacatggtg gcggcgccga ctcgcgacga
     8101 cgtcgtgtgc gcgcagtggc ttcctggcga cacgacagag gggcgcagca cggtcttgac
     8161 gggttctgcg gttcctctcg ccaccgggca ggcgcccgtc ggcctggccc aggctgacgg
     8221 cctcggcccc aacgctgacg cggtattcgt gccgccggga cggagcgtct acgccgaatc
     8281 cacgcatcgg tacctgatcg ccgacaccgg tgtgcggttc ggcgtgccag acgccgaatc
     8341 cgctgttgcc ctgggactcc cgccgcgcgc gttgccggca ccatggccga tcctgaagtt
     8401 gctcgccgat ggcccggaac tcagccggtc ggcggcacta gtcgcgcacg acggtgtggc
     8461 gcccgacccg cgcgctatcg cgctgcccgg caccgcggtg gcgccgcgct aatcggttcg
     8521 ccggggagat caatgccaga gcggcggcca gaaacgccgc gcaccccgcg acgcccgtga
     8581 acgccgttga gcggccggac gtttcccgcg gcggtggcgg ggccgacggt gccaccgggc
     8641 gttgcgccca ggacggaggt gcgctcggtc ggccgtcagt aagtgccgcg agtgggtcga
     8701 caacaccgtt tccgacgtag gggtcccagc catcgggtgg ccggtgtgcg gtggcctcga
     8761 tgcgctgcat cacctgccgt gccgacaagt cgggaaatcg cgcccggacc aacgccgcaa
     8821 gcccactgac aaccggcgct gcgtagctgg tgcccgcaat ggctgagccg gttccgctgc
     8881 ggtacgcgtt ggccacgccg gtgtggtccg ggttcagtga cacgaccgcc tcgccgggtg
     8941 ccgcgacgtc cacccaagga ccagccaggc tgaatgtcga cggggaccca tcggtgctga
     9001 cggacccgac ggtgaggacg taatcgtcat accagccggg gctgaccgcg acatctgctg
     9061 tctcccagtc aggttcgggt cggcgcgcgg gatcgggcgc tgggttctgc tgcgggcaat
     9121 tgccacccgg gccgacgttg ccggccgcga cgaccacgac ggcgtctttg gcgtcgacgg
     9181 cgtacgcgac ggcggcgccg agcgcacgat cgcggagcgc cgccccggcc gtgcacgcga
     9241 ccgacgagat gttgatgacg gtcgcgccca agtcggcagc ggtgcgcacc gccatcgcaa
     9301 gcgtgtcgac atcgccgacg ccgaacgaac gcccgctgac cggcccgtac ttgacgctgg
     9361 actgccggat cgtgatcacc gacacccccg gggcaatacc ggcaaagccg tccgattctg
     9421 ttgtggctgc gatgattccg gcaacgagcg tgccatgagc gtcgcaatcc tcgcggccgt
     9481 ccccggtgga cacgtagtca ccgcccggga tcaggcgggg gagccgcgga tgcgtggaca
     9541 ccccggtgtc gatcaccgcg accttctggc cgtcgccagt ggtcaacgcc cacagcgcgg
     9601 gcaggtcaaa cggcgtgagt tgcgtcgcgg tgggattcag ttggccatcg aatattgctg
     9661 tggtgcaatc ggattgcttc tcggtgggag tgtgcggctt cggtggagct ggcggcggca
     9721 gcagcgagtc gtcgatcgcg ggtggccgtt ccgcggacgc cgccggcaac ggtgccagcc
     9781 cgggcaggcc cacggcgagc gccgcggcca cgaataccct caacatggtg ctcatcagaa
     9841 gctcagccca cgcaccgcgg cgaacacccc gcacacccag caggccagcg ggatcaccaa
     9901 gccaagcgcg gcgtattcgg tcaggtcgac gcacctgcgt atcagaggcg acggcgtcat
     9961 caccgccgac gaacacaccg tcgacgccgc tgcgatgccg gccgcgccga gagcgatcca
    10021 gtgcgcgtgc tgcgcccact tgccgaccga ccagatgaac accgctgcga aacacgatgc
    10081 gccgctgaca agcagtgccg ctacttgcac gacgtccacg tgggtgccgg cccgcagcgc
    10141 gagcatcaca ccgatggccg cggcgaacgc gacaccgcag aacgcgccgc catggacggc
    10201 gagggaggcg ccgagcgcgg cggcggcgct gaatccgcag acgagccccg tgatgatggc
    10261 gtgggcatgg ctggcgtgct ccgcgatgct ttcgagtgcc gcgccgtcgt cgcgaaccgg
    10321 cgcaccgggc agacgcggaa tcggcacacg cccggtcgat atcgccagcc gtggggtgag
    10381 gacaagcagt ccggctgccg ccgcggcgag cacggcgccg gtttgcgcga cgtcaacagg
    10441 tgcgacgagg tgcacccatg ccgctgccgc ggacgagaac ccaaacgacg ccagcccgct
    10501 gaaaactgct gtgccacggc cgatatagcg agacgccaac accgacacgg tggcgctcac
    10561 tgcgctggcg agcatgagtt tcggtgccgt cgcgcctcct gggaccacga ggtaaccggc
    10621 tacggtcgcc gatgccgccg cgcaggcgcc gagtgtcacc gcggtgagcg gttgccggta
    10681 catccgccca tacgccaccg cggcggtgag agcgacggcg gagagcaatc cggcgatcac
    10741 cggcgagtcc gacgacggcg ctcgcagcag cgcgtacgcg atgagcccgg tgcaacagag
    10801 cgtgccgaca gagaccgcca cgcgggtgag ggtcggcgtc cagcgcggga tcttctccag
    10861 cgatgacgcg atctcggcga ttgcgtcgtc ggcgggcggc ggggcgaccg cagcagagac
    10921 acgccccagc cacaggacgt cgccgtcgcg aaccccgttg tcccgcagag aagcccgcgg
    10981 tgcgagcggc cgtccgtcgg tgcgtgacaa ctgccagtcc gacggaattt cgtcccatcg
    11041 gcgacggccg gccgccccgc tgctggccgc gacgtggtcg acgataggcg gaatcacttc
    11101 tgtcacagtg aaatcggccg gtaggacgaa atcaagcctg cccgattcga actgcacccc
    11161 gatacggcat atgtcagacg tgactgtcct cgccatttca tccccccggt cgtgagcggc
    11221 cccgacccgc ccgccaccgg cacggtaacc gagcggggcg caggcgggtg aagaccttgt
    11281 ggacaacgcc ggcacctgtg gatagccggt tgcggcgcgg tcgccgccgc ggcagcgtgt
    11341 cccgcatgcc ggactggcac tcgggcccgc gcatggccgc accggccatt cccgaaggcg
    11401 aggtcgtcat tgaatcgccg aacgaaccgc cgcgcgcgct accggcgagc cccgtcgtgc
    11461 ggatgctgcc cgtcgtcatg gtcgtcgcga tggcgggcat gatggtgctg tttgtcagct
    11521 cgggcgctgc cgcggcccgc aatccaagtt cgcttctttt tccggcgatg atggtggtgt
    11581 cgtcgatcgg gatgatcgcg cacggccgcg gagcgggtgc ccgcgcggct gaactcgacg
    11641 agggccgcaa ggattacctg aggtatctcg gacgggtacg cgacgaggcg ttcgaaagtg
    11701 ctcgctgcca acgggtgtca ctactctggc agcatccgga tccggctgcg ctgtggacgc
    11761 ttgccggcac gcgccgaatg tgggaacgag gcagagccga ctccgatttc tgccatgtcc
    11821 gtgtcggaac aggcaggcag cggtccgccg caccgccgac tgcaccgaac ctcgggccgg
    11881 tcgatcagct cgagccggtg agtgccatgg cgttgcgtga gttgattcgc gcgcattcag
    11941 tggtcgacga ggttccgatc gcgttggcgc tgacgcgatt cggcgcggtc gcggtcgatg
    12001 gcgacggcgc ccgagatctg atgcgtgcga tggtgtgcca gctcgcggtc ctgcacggtc
    12061 ctgcgcttct tgcgattgtc gccgtcgtgg acgacgcggc cgcagcggag tgggactggt
    12121 tgaagtggct gccgcatcgg ggtgattcgg cggcgcacac ggtggtggtc gtcgacggag
    12181 gcgccgtgtc cggtgtcgca ccttttgccg agcgcggcga cgtcacgatc ctcgagcggg
    12241 gtcgtcgatg ccagggcgag ggatcacgac catgcctgcg actcgacgtc tgcgccgacg
    12301 aactcgccgt gcttggcgac gtgcgagagg tggtcgcccg gccggacgcg atggcggttt
    12361 cggaagccga gctgtgtgcg cgtcggctcg cgccgtaccg gtcggccgcg gaccgactgc
    12421 cgacgtcggc gcccggtgcc gactgggctc aggcaacggg catcggcgac ccgtgggcgc
    12481 tgcaaccgga aaccatctgg cgacagcgca agccgcacaa cagacttcgg gtcgcgatag
    12541 gcgtcgcggc ggacggttcg gctgtcgagc tcgacctcaa agaggctgcc gagaatggca
    12601 tgggtccgca cggtttgtgc gtcggcgcca cgggttcggg taagtccgaa ttcctgcgca
    12661 cactggcact ggggctgatc gccacacatg atcccgaggc cctgaacctg gtcctcatcg
    12721 acttcaaggg cggcgcaacc tttctgggac tggaacgtgc ccggcacgtg tcggcggtga
    12781 tcaccaacct ctccgacgag gcccatctgg tcgcacggat gaaggacgca ctcgccggtg
    12841 agatgaatcg gcgtcaggaa ttgcttcgaa cggcaggcag gttcgcgagc gtgaccgact
    12901 acgagtgggc gcgcgaggcg ggagcgccac ttcggccgct gcccacgctg ctggtgatcg
    12961 tcgacgagtt ctccgaactg ctcacccagc accccgactt cgccgagatg ttcgtcgcga
    13021 tcggtcggct gggcaggtcg ttgcgagtac acctgcttct ggcgacccaa cggctcgacg
    13081 agggcaggct gcgcgggctg gaatcccact tgtcgtaccg tgtgtgcctg aaaaccttct
    13141 cggccaacga atcccgcgcg gtgatcggcc gcccagacgc cttccagctg cccgccaccc
    13201 ctggtgcggc gttcttgaaa gttggatccg acgagcccgt cgcgttccag accgcgtacg
    13261 tctcgggtcc gtgtgtcgtg ccagcgacgc cgacgcggcc gcccaccacg cccacggtgc
    13321 gactgttcac cgccacgccc atcgcgaccc ctgcggcgcc cgaacgggct cacaagcacg
    13381 cggccggcac cgtgctggac actgtgctcg accggctggc gggacacggc acaccggcgc
    13441 atcaagtgtg gttaccaccg ctgaccgcgt caccgaccct cgacgaatta cttcgcacag
    13501 gccacggagt gctcgaggtg ccgatcggtt tcgtcgacgt gccgttcgac caacggcggg
    13561 atccgctcgt cgtcgaattg gcaggagcgg caggcaacgt cgcgatcgtg ggagccccgc
    13621 agtcgggtaa gtcgaccgcc gtgcggacgc tggtgactgc aatagccgcc actcatgcgc
    13681 ccgacctggt gcaaatgtac tgcctcgact tcggcggcgg cgcgttgtct gctcttgggg
    13741 cgctcccgca cgtcggtgcg gtggcgggac gggccgatcc ggacctcgtg cgcaggaccg
    13801 tcgcggagct gagcgcgctg ctgcgcgcac gcgaggcggc gtttcgcaaa ctcggcatcg
    13861 attcgatggc cgactaccgc cgtcgtcgag cagccggcga tccgagcact gcggatgacc
    13921 cgttcggtga cgcgttcctg attgttgacg gatgggcaag cctgcgcgac gatttcgacg
    13981 ctctcgagct gccgatcacg gcgctggcta cccgcggctt gtcgtacggg gtgcacgttg
    14041 tgctgaccgc atcgcgctgg gcggagattc ggccggggct gaaggatcag gtcggcaccc
    14101 gcattgagtt gcggctcggc gacgccggcg attcggagct gaatcgcagg gcagcccagc
    14161 atgttccata cggccgcccg ggtcatggca tcacccgaga cggcatgcac atgctcatcg
    14221 ggctgccgcg gcttgacggg aagcatgagg ccacaggcct ttccgcggcg atcggcgatg
    14281 cggcagcgat gatccgcgct cagttcggtg actgtgcggc gccaagcgtg cgccttctac
    14341 cagaccgcgt cgaccacact gcgctcatcg ggggacaacc gccgacgcag atgatcatcg
    14401 gtgtcgatga agacgaactc gccgctgtca cagtcgattt cgccgaaaat cagcacattc
    14461 tcatcctggg cgacgtcgag tgtggcaaga cggcggcgct tcggctggtc tgcgcagaat
    14521 tggtccgcac ccgcagcccc gagcaggcgc agctgatggt cgtggactac cggcgctcgc
    14581 ttctgggcgt ggtcgagtcc gaccacctcg ccggctacgc catctccggt gctgttctca
    14641 ctgcgcagct accggcgttc gtggaccgcc tgcgggcccg gatacccggc ccggatgtcg
    14701 accagcggca actgcgaacg cggtcatggt ggtcgggtcc taacgtgtat ctgatcgtcg
    14761 acgactacga cctcgtcagc accgacaccg gcaacccgct ggcgcctttc atcgaattgc
    14821 tgccgcacgc aaaagatctc ggcttacacg tggtggtagc gcgacgcgcc ggaggcgcac
    14881 gccgcgcctt gttcgagccg ttgctcgcgc ggctgcgcga actcggctgc ctcggggtga
    14941 tgatgagtgg gagcccggac gaaggagtgc tgctcggcgc ggcgcgtgcc ggcccgttgc
    15001 cgccgggacg cgcgcgcctg atcacccgcg ccggcgagcg tgtcgtccaa ctgggctgga
    15061 tcccgccgtg catgtgagcg ctgtcgtcgt cggcccatgc ggcgtgcgcg catcgggtgt
    15121 cttggcgacg gaacaggccg gcctggccgc cgcggcgctg gacgccatcg acgacgagtt
    15181 ggccttggtc gacgagcggc cggtagccgt cggcggcctc tggcgcgagg tcttcgcggc
    15241 gttgcttgac ggcggaaaag cggtgctggt ctgcccatcg tggtggtcgg atcggcggat
    15301 cgccaccgtg tcggacgctg cgcacgccgt ggtcgcggac gtcgaggtgg cgcggcggtc
    15361 ggcgatgctc gtgtcggcgg tcgcgcggca gtcggctgtc gtcgtcgaga tcgccggcga
    15421 cttcgtcgcg ctgagccggc cgcccgccga ccggccggtc aagatcgtgt cgagggatgc
    15481 gaggccggcg gacgtcgccg acgaggtggt gcggtgggtc gcgtcggcgg cggtggtcga
    15541 ccgcgccgcg ggcgtcggcg gagcggtgga gctcggcgcg atgatcgccg accggctacg
    15601 ggcgtccggg acaccggtga ctgtcgtcga cgacgagcgg ctgctgcgcg cggcacagaa
    15661 attcgccgcc gacgacccga cggtgccctg cgaggcgatc aggccacgcc ggcccagagt
    15721 ctggccgtgg gtgggcgctg ccgcagcggc gggggtgacc atcaccggga tcgcactggc
    15781 cagtggcccg tcgccgacac cgccgacgac cctgctggtc gagggcagag tggtggtgga
    15841 agtgccggtg acgtggactg cgcggcgcat caccggcgga ccaggctcgg cacggctgca
    15901 ggtggcgtcg ccgaaggatc cgcacgccgc cgtgcacgtc acccagtcgc tcgtgccgag
    15961 cggcgaaacg ctgcaacgaa cggccgacac actgcgtcgg gcgatgcgcg acgagccgcc
    16021 cggcgtcttc gtcgacttca accccgccga ccgccgaggt gatcgggagg cggtgacgta
    16081 tcgggagatc cgcgacggcc acgacatcgg atggacggtg ctgctcgatg ggcaggtccg
    16141 gatcagcgtc ggctgccaga gcgcgcgcgg tgccgaagag tccgtccgcg cggtgtgcga
    16201 ggtcgccgtc gcgtcggccc gcgctgtcgc cgaatctgtg ggaaccgttg cgccgcagcg
    16261 tcagtccaac aacacataac ccgatgtgaa aggaccgaca tgacctcacc cggagcgggc
    16321 agcctcacca ccgacttcga cctgatgcgc gatgtcgccg gcaagatcga cgcccgcaac
    16381 gacgagatca ggacgatgct gcaaggcttc atcggccgga tgacgagtgt gccgccatcc
    16441 gtgtggggcg gtaacgccgc cactgctttc aaaggtgtgg tcgaccgctg gaacagcgaa
    16501 tcgacgaagt tgcagcaggc actgcagggg attgcagaga ccatccgcgc caacgagcag
    16561 tcgctgcgtg aagccgccga cacgcattcg gcccagatcg gcgccgtcgc cggaaacctc
    16621 tgagtggagg cctaacgcat ggatacgtcc ctgtcttaca acttcgccga aatcgagttc
    16681 tcggtacgcc a
//
LOCUS       DASWBF010000310        10901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1142577,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000310 DASWBF010000000
VERSION     DASWBF010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142577"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /gene="mshC"
                     /locus_tag="VF874_11335"
     CDS             complement(<1..1103)
                     /gene="mshC"
                     /locus_tag="VF874_11335"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HEX9176634.1"
                     /translation="MQSWSSAPVPVLPGRGPELRLYDSSDRQVRPVATGRTATMYVCG
                     ITPYDATHLGHAATYLAFDLVYRVWLDAGHEVHYVQNITDVDDPLFERAHRDGVDWRE
                     LGSREIDLFRDDMAALRVLPPREYVAATEAIDEVVELVEKMLASGAAYVVDDPDHPDV
                     YFRADATPQFGYESGYDRDTMARLFAERGGDPLRPGKADELDALLWSAAREGEPSWPS
                     PFGPGRPGWHVECAAIALSRIGTGLDIQGGGSDLIFPHHEFSAAHAECVTGERRFARH
                     YVHAGMIGWNGHKMSKSLGNLVLVSQLRADGLDPAAIRLGLLAGHYRSDREWSADVLG
                     GAQARLARWRQAVALPSGPDAADVLARVRRYLA"
     gene            complement(1128..1889)
                     /locus_tag="VF874_11340"
     CDS             complement(1128..1889)
                     /locus_tag="VF874_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9176635.1"
                     /translation="MTLTDAALAAELADAAGKLLLEVRDEFGFNDPWVLGAAGDKRAN
                     ALILERLRDERPDDGVLSEEAHDNLRRLNKDRVWIIDPLDGTREFSTRGRDDWAVHVA
                     LWQGGAEITDAAVALPAQGELYRSDTVRADTSDSGAADRPIRVAVSTTRPPSLLYWIR
                     HEVALQPVPIGSAGAKAMALVRGDVDAYLHAGGQWEWDSAAPAGVVLAAGLHASRVDG
                     SPLRYNQLDPYLPDFLMCRTEAAPTLLDAIRRAVW"
     gene            2164..2364
                     /locus_tag="VF874_11345"
     CDS             2164..2364
                     /locus_tag="VF874_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9176636.1"
                     /translation="MAAPVAWVPQPGAEYHRILGQLITTYDMSGNLMPDAMLAALAIE
                     HGVTVYSTDTDFAKFRWENPLG"
     gene            complement(2461..3000)
                     /locus_tag="VF874_11350"
     CDS             complement(2461..3000)
                     /locus_tag="VF874_11350"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEX9176637.1"
                     /translation="MSSWMVTAVLLVGLASACSPHRESADTSPTSATTVQPQSIEVPL
                     YEQFESANAEGANGTPIDARLVGKSYPLSTGMWVGCRGTAATATYRLDRTFTRLTATV
                     GLQEQAPDGLAVRVTISGDGQVLSEFTVRKTATMSVDLKVDSTDSLVVAAILEQGICG
                     QADVPYGALGDAVLTTNRD"
     gene            complement(3029..3835)
                     /locus_tag="VF874_11355"
     CDS             complement(3029..3835)
                     /locus_tag="VF874_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX9176638.1"
                     /translation="MTPSPEALRRGELTVIGRIRSASNATFLCEAEWAGQRTHCVYKP
                     VAGEQPLWDFPDGTLAGREIAAYLVSESLGWNIVPHTIIRDGPAGRGMVQLWIEEPDD
                     SSADIEHTTPDPVDLFPAGNVPSGYLAVLRAYDYSGDEVTLVHSDDVRLFRMAVFDVL
                     INNADRKGGHILIGLDGNTYGVDHGVSLHVDDKLRTVLWGWAGKPIDDDTLAAVEKLR
                     TALRDALGEALRAHITRHEIVALRRRAEALLDNPVMPTPDRRRPIPWPAF"
     gene            complement(3819..4397)
                     /locus_tag="VF874_11360"
     CDS             complement(3819..4397)
                     /locus_tag="VF874_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HEX9176639.1"
                     /translation="MARAIHVFRSPDRFVAGTVGQPGNRTFYLQAVHDSRIVSVVLEK
                     QQVAVLAERIGALLTEVHRRFGTPIPPDTGEVEDLSPLVTPVDAEFRVGTMGLGWDSE
                     AQTVVVELLAVTDTEFDASVVLDDAEEGPDAVRVFLTPESAREFATRSNRVISAGRPP
                     CPLCDEPLDPAGHICVRTNGYRRGALADDTES"
     gene            complement(4457..5146)
                     /locus_tag="VF874_11365"
     CDS             complement(4457..5146)
                     /locus_tag="VF874_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9176640.1"
                     /translation="MTVILLRHGRSTSNTAHVLAGRSDGVDLDERGREQADALVGRLS
                     SLPIRALVCSPLLRCLRTIEPLSAALGVESVVDERLSEVDYGQWTGRNLGDLVKEPLW
                     KVVQAQPSAAVFPDGEGLAQVQARAVAAVREHDRRLADEHGGDALWVACTHGDVIKAV
                     VADALGVHLDSFQRIVADPASTSVIRYTPMRPFVLHVNHTGADLASGLTAPPADGKVP
                     GSDAVVGGSTE"
     gene            complement(5143..5973)
                     /locus_tag="VF874_11370"
     CDS             complement(5143..5973)
                     /locus_tag="VF874_11370"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9176641.1"
                     /translation="MSWWQVIFLAVLQGLTEFLPISSSGHLAIASRVFFADDAGASFT
                     AVSQIGTELAVLLYFARDIWRIAKAWVNGLVVKEKRDVDYRMGWYVIIGTIPIVICGL
                     LFQDMIRTYVRSLWVVAIALVVFSAVIAAAEFAGRQTRDEEDLRWYDAVLIGLAQTLA
                     LIPGVSRSGAAISAGLFLGLNRELAARFGFLLAIPAVLGAGLHELPNAFKPVSEGMSA
                     TGPQLLVSVVIAFVVGLAAIAWFLRFLVNHSMYWFVVYRVALGVGVMVLLGTGVVAAT
                     "
     gene            complement(6012..6311)
                     /locus_tag="VF874_11375"
     CDS             complement(6012..6311)
                     /locus_tag="VF874_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176642.1"
                     /translation="MTLAANSECSGKEFVVDDVTAGVHANGFGHISDGRSFAFFSHHN
                     ELVVEVYRPRLSGPVPQPEDVIAVATRRLTDIDVTDERSLVAAVRDAVANAQPVR"
     gene            6462..7442
                     /locus_tag="VF874_11380"
     CDS             6462..7442
                     /locus_tag="VF874_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176643.1"
                     /translation="MAVCFVALSAVACSANPVDDTPPTIAPASPAVSPQVTTAPAGQV
                     RPFPAKAKEAVFDAATASLVVLGADGVTIMPAGDGAPRNVAVPSPPSALTGDGSGAVY
                     LSTRGGYFRVDVRTGQVATMKVAGHEGTDFTAIARRSDGRLVLGSADGAVYTLSSDTA
                     VAASVKIFARVDALVTQGNTAVVLDRGQTSVTTIDPDGSGAQHALRAGEGATTLAADS
                     AGRLLVADTRGGSLLVFGTDPLMMRQSYPVRDAPYGLVGTRTLAWVSLTATNVVVGYD
                     LATGIPVEKVRYPTVRQPDVLAFDDATNTLYVASSSGDGIQVINDAGRPR"
     gene            7466..7663
                     /locus_tag="VF874_11385"
     CDS             7466..7663
                     /locus_tag="VF874_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HEX9176644.1"
                     /translation="MSDDYEWVPLRLPPDVTRLSASTRLSIEAEYRGWELTRVRLYTD
                     GSRRVLLRRRKTDRPSDQPGL"
     gene            7663..8697
                     /locus_tag="VF874_11390"
     CDS             7663..8697
                     /locus_tag="VF874_11390"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX9176645.1"
                     /translation="MLYRALRRVMFLVPAERIHTWVFAVLRLATAVAPARRMLHRLLA
                     PHDPALASTVFGVRFPGPLGLAAGFDKDGTGLDTWGALGFGFAEVGTVTAHPQPGNPS
                     PRLFRLPAERALLNRMGFNNHGACELALRLARHVPDVPIGVNIGKTKLADDAVGDYRA
                     STRLVGALADFVVVNVSSPNTPGLRDLQAVESLRPILTAVQSETTTPVLVKIAPDLSD
                     TEIDDIADLAVELGLAGVVATNTTVRHQLGEGGVSGAPLAPRSLEVLRRLYARVGGKL
                     ALISVGGIETADDAWQRITAGASLLQGYTGFVYGGGLWPKHIHDGIAQRLHAGGFGSL
                     GEAVGSAARR"
     gene            complement(8694..9230)
                     /locus_tag="VF874_11395"
     CDS             complement(8694..9230)
                     /locus_tag="VF874_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX9176646.1"
                     /translation="MAFPYNPYDFLPKLPTFTLTSADISDGQPLKTPQVSGIMGAGGE
                     DVSPQLSWSGFPEETRSFAVTVYDPDAPTASGFWHWAVANLPATCTELPAGVGEGSEL
                     PGSAVTLVNDAGMRRYIGAAPPPGHGTHRYFVAVHAVKVEKLELDEDASPAYLGFNLF
                     MNAIARAVIHGTYEQKDS"
     gene            complement(9250..10593)
                     /locus_tag="VF874_11400"
     CDS             complement(9250..10593)
                     /locus_tag="VF874_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9176647.1"
                     /translation="MVGKGTVTGAVDEVVDLVSALIRFDTSNTGDPATTKGEQECAQW
                     VAEQLHEVGYETEYIEAGAPGRGNVFARLPGKDSDRGALLIHGHLDVVPAQAADWSVH
                     PFSGAIEDGYVWGRGAVDMKDMVGMTIAVARHFKRSGIVPPRDLVFAFVSDEEAGGKY
                     GCKWLVENRPDLFDGVTEAIGEVGGFSLTVPRRDGGERRLYLIETAEKGMLWMRLTAH
                     GRAGHGMLIHDDNAVTAVAEAVTRLGRHQFPLVMTDAVEQFLQAVAEETGYTFDVNSP
                     DLDGAIAKLGSIGRLIAATLRDTANPTMLNAGYKANVIPATAEAVVDCRVLPGRLSAF
                     EREVDELIGPDVTREWIVDLPSYQTTFDGRLLDAMNDAILATDPEARTVPYMLAGGTD
                     AKHFARLGIRCFGFAPLRLPPELDFSALFHGVDERVPVDALEFGARVLEHFLLNC"
     gene            10682..10767
                     /locus_tag="VF874_11405"
     tRNA            10682..10767
                     /locus_tag="VF874_11405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10715..10717,aa:Leu,seq:gag)
BASE COUNT         1794 a         3715 c         3651 g         1741 t
ORIGIN      
        1 tcggcgaggt agcggcgaac gcgtgcgaga acgtcggcag catccgggcc cgacggcagc
       61 gcgacggcct ggcgccagcg cgccagcctc gcctgcgccc cgccgaggac gtcggcactc
      121 cactcccggt cggaacggta atgcccggcc agcagcccca gccggatcgc ggccgggtcg
      181 aggccgtcgg cgcgcagttg cgacaccagc accaggttgc ccaggctctt cgacatcttg
      241 tgcccgttcc agccgatcat ccccgcgtgc acgtagtgac gcgcgaaacg ccgttccccc
      301 gtgacacatt cggcgtgcgc ggcggagaac tcgtggtgcg ggaagatcag gtccgacccg
      361 ccgccctgaa tgtcgaggcc ggtgccgatg cggctcagcg cgatcgccgc acactcgacg
      421 tgccagccgg gccggccggg accgaacggc gacggccagc tcggctcgcc ctcgcgggcg
      481 gccgaccaca acagcgcgtc gagctcgtcg gccttgccgg ggcggagggg gtcgccgcct
      541 cgttcggcga acaaccgcgc catcgtgtcg cggtcgtagc cggactcgta gccgaactgc
      601 ggtgtggcgt cggcgcggaa atacacgtcc ggatggtcgg gatcgtcgac gacatacgca
      661 gccccggatg ccaacatctt ctcgacgagc tcgacgacct cgtcgatggc ctccgtcgcg
      721 gcgacgtact cgcggggcgg caggacccgc agcgcggcca tgtcgtcgcg gaacaagtcg
      781 atttcgcggc tccccagctc gcgccagtcc acgccgtcgc ggtgtgcccg ctcgaacaac
      841 ggatcgtcga cgtcggtgat gttctggaca tagtggacct cgtgcccggc gtcgagccag
      901 acccggtaaa ccaagtcgaa ggccaggtac gtcgccgcat gccccaggtg ggtggcgtca
      961 tacggcgtga tgccgcagac gtacatcgtg gcggtccgcc cggtggctac cggccgcacc
     1021 tgtcgatccg agctgtcgta caaccgcagt tctggtccgc ggcccggcaa caccggcacc
     1081 ggggcggacg accacgattg catgctgtcg agggtaagcc gggcgggtta ccagaccgct
     1141 cgccggatgg cgtccagcag cgtcggcgcc gcttctgtgc ggcacatcag gaagtcgggt
     1201 aggtacgggt caagctggtt gtagcgcagc ggcgagccgt cgacccgcga cgcgtgcagg
     1261 cccgcggcca gcaccacgcc ggccggcgcc gcggaatccc actcccactg accgccggcg
     1321 tgcaggtagg cgtcgacgtc gccacgcacg agcgccatcg ccttcgcgcc ggccgatccg
     1381 atcggcaccg gctgcagggc cacctcgtgg cgtatccagt acagcaggga cggcggcctc
     1441 gtggtgctga ccgccacccg tatcggccgg tcggccgcgc cggagtcgct cgtgtcggcg
     1501 cggacggtgt cgctgcggta gagctcacct tgcgccggca gcgccaccgc ggcatcggtg
     1561 atctcagcgc ctccctgcca cagcgccacg tggaccgccc agtcgtcgcg gccgcgggtg
     1621 gagaactcac gcgtgccgtc gagcggatcg atgatccaga cgcggtcctt gttcagacgg
     1681 cgcaggttgt cgtgtgcctc ctcgctgagc acgccgtcgt cgggccgctc gtcgcgcaac
     1741 cgctccagga tcagcgcgtt ggcccgtttg tcgcccgccg cgccgagcac ccacgggtcg
     1801 ttgaacccga actcgtcgcg cacttcgagg agtagcttcc cggcggcgtc ggcgagttcg
     1861 gcggccagcg ccgcgtcggt cagggtcacc ggtcaagtat tgctgaccca ctctcgccga
     1921 cgacgtgctg gcggccgtcg agaagatccg gcgcgagcgc ggtatcggac tgagtgaggc
     1981 agtgaacgag ctgatccgag tcggtctcac gaagtccgag acacgtaagc ctttccgtca
     2041 gaagacctac gacatgggca aggcatacgt cgattactcc aatgtctacg cgttgccacc
     2101 catcccagga tcttcccgcg accgctcacc ccgggaacgg cattcgatgt cgtcaccaac
     2161 tggatggccg cgccggtcgc ctgggttccg cagccgggcg ccgagtatca ccgcatcctt
     2221 ggacagctca tcacgacgta tgacatgagc ggcaacctga tgccggacgc gatgctcgcg
     2281 gcgctggcca tcgaacacgg tgtgacggtg tactccacag atacggattt cgccaagttc
     2341 cgctgggaga acccgctggg gtgactatgg cgttgaccgt gcgaatttct acgcgctgac
     2401 cgcgtttcgc gtacggcacc gcacaacggc gggcaaggcg gcgggggtct cagaatccgt
     2461 ctagtcgcgg ttcgtagtca ggacagcatc gccaagagcg ccatatggga catccgcctg
     2521 gccgcaaatc ccctgttcga ggatcgccgc aacgacgagg gaatccgtgc tgtctacttt
     2581 caagtccacc gacatggtcg ccgttttgcg gacggtgaac tcgctgagga cttgaccatc
     2641 gccagagatc gtgactctta cggccaaacc atcaggtgct tgctcctgca ggcccacggt
     2701 cgcggtcagc cgagtgaatg tgcggtccag ccgatatgtc gcagtcgcag ccgtgcctct
     2761 gcaaccaacc cacattccgg ttgagagggg atagctcttt ccaactaacc tcgcatcgat
     2821 cggtgtgccg ttcgccccct ctgcatttgc agactcgaac tgttcataca gcggcacttc
     2881 gatggattga ggttggacgg ttgtggcgga tgtgggcgat gtgtcggcgc tctcgcggtg
     2941 tgggctgcat gcacttgcca acccgaccaa caagactgcg gtgaccatcc aggaactcac
     3001 ggcgcacttc atgatggagg tcgcaccctc agaacgccgg ccacgggatc ggacgtcgcc
     3061 ggtccggcgt cggcatcacc gggttgtcca gcagcgcctc cgcgcgcctg cgcagcgcga
     3121 cgatctcgtg ccgggtgatg tgcgcacgca acgcttcccc gagcgcgtcc cgcagggccg
     3181 tccgcagctt ctcgaccgcg gccagcgtgt cgtcgtcgat cggcttgccg gcccaccccc
     3241 acagcaccgt gcgcaacttg tcgtcgacgt gcaggctcac gccgtggtcg acgccgtatg
     3301 tgtttccgtc cagtccgatc aggatgtggc cgcccttgcg gtcggcgttg ttgatcaaca
     3361 cgtcgaagac ggccatccgg aacagccgga cgtcgtccga gtggaccaac gtgacctcgt
     3421 cgccggagta gtcgtaggcc cgcagcaccg ctaggtagcc cgagggcacg ttgccggccg
     3481 ggaacagatc cacggggtca ggagtggtgt gctcgatgtc cgccgacgag tcgtcgggct
     3541 cctcgatcca cagctgcacc atgccccggc ccgccggccc gtcgcgaata atggtgtgcg
     3601 gcacgatgtt ccagcccagt gactccgaca ccaggtaggc ggcgatctcc cggccggcca
     3661 gggtgccgtc ggggaagtcc cacagcggct gctcaccggc gacgggcttg tacacgcagt
     3721 gcgtgcgctg ccccgcccat tccgcctcac acaggaacgt tgcattgctg gccgagcgga
     3781 tccgcccgat gaccgtcagc tcaccgcggc gaagcgcctc aggactcggt gtcatcagcc
     3841 aaagcgccgc gccgataccc gttggtgcgc acgcagatgt ggccggcggg gtcgagcggc
     3901 tcgtcacaca acgggcacgg cggacggccc gccgagatga cgcggttgga ccgggtggcg
     3961 aactcccgcg ccgactccgg cgtcaggaac acccgcaccg cgtcgggccc ctcttctgcg
     4021 tcgtcgagca ccaccgaggc gtcgaactcc gtgtcggtca ccgcgagcag ctcgaccacg
     4081 acggtttggg cttccgaatc ccagccgagg cccatcgtgc cgacccggaa ctccgcgtcg
     4141 accggggtga ccaacgggct cagatcctcg acctcgccgg tgtccggcgg aatcggggtg
     4201 ccgaagcggc ggtgcacctc ggtgagcagc gcaccgatcc gctctgcgag caccgccacc
     4261 tgctgctttt ccaacacgac cgagacgatc cgcgaatcgt gcacggcctg caggtaaaag
     4321 gtgcggtttc cgggctgccc gacggtcccg gccacgaatc ggtcgggtga gcgaaacacg
     4381 tgaattgcgc gggccatgtc gtatccaaaa taccggtaga tgctgctcgt ccgtagttct
     4441 gaagccgtct cgttagctat tcggtcgagc cgccgaccac ggcgtcagag cccggcacct
     4501 ttccgtcagc gggcggtgcc gtcagcccgg atgcgaggtc ggcgccggtg tggttgacgt
     4561 gcaacacgaa cggccgcatc ggcgtgtagc ggatgacgct cgtcgacgcc gggtcggcaa
     4621 cgatgcgctg gaaactgtcg aggtgcaccc caagcgcgtc ggcgaccacc gccttgatca
     4681 cgtcgccgtg cgtacacgcc acccacagtg cgtcgccgcc atgctcgtcg gccagccgcc
     4741 tgtcgtgctc acggaccgcg gcgaccgccc tggcctgcac ctgcgcgaga ccctccccgt
     4801 ccgggaacac cgcggcgctg ggctgggcct gcacgacctt ccacaacggc tccttgacca
     4861 ggtcgccgag gttgcggccc gtccactggc cgtagtcgac ctcggacagt cgctcgtcga
     4921 cgaccgactc gacgcccagc gcggcggaca gcggctcgat cgtgcgcagg cagcgcagca
     4981 gcggcgagca gaccagcgcg cggatcggca gtgaggagag ccggcccacc agcgcgtcgg
     5041 cctgttcacg cccacgctcg tcgaggtcga cgccgtcgga gcggcccgcc aacacgtgtg
     5101 cggtgttcga cgtcgagcgg ccgtgccgca gcaggatcac cgtcatgtcg cggccaccac
     5161 cccggtgccc aacagcacca tcacacccac cccgagtgca acccggtaaa cgacgaacca
     5221 gtacatgctg tggttcacca ggaatcgcag gaaccacgcg atcgcggcaa gaccgacgac
     5281 gaacgcgatg accaccgaca ccaatagttg cggaccggtg gcgctcattc cctcgctgac
     5341 gggcttgaac gcgttgggca gctcgtggag accggcgccc agtactgctg ggatggcaag
     5401 cagaaaaccg aagcgcgcgg ccagttcccg gttcaatccg agaaagaggc cggcgctgat
     5461 cgcggcgcct gagcgagaga cgccgggtat cagcgcgagg gtctgagcca gcccgatgag
     5521 aaccgcgtca taccaccgca ggtcttcctc gtcgcgagtc tgtcgcccgg cgaattcggc
     5581 cgccgcgatg accgcggaga acaccacaag tgcgatcgcg accacccaca ggctgcggac
     5641 gtacgtgcga atcatgtcct gaaacagcaa accgcaaatg acaatcggga tggtgccgat
     5701 gatcacatac cagcccatgc ggtagtcgac gtcgcgtttc tccttgacca ccagcccgtt
     5761 gacccatgcc ttcgcgatgc gccagatgtc gcgggcgaaa tacagcagaa cggccagttc
     5821 ggtaccgatc tggctgaccg ctgtgaacga cgcgccggcg tcgtcggcga agaacacccg
     5881 cgacgcgatc gccaggtggc ccgacgacga gatcggcaga aattcggtca gcccctgcag
     5941 gacagccaaa aagatcacct gccaccagga catggccgcc gtctccacgc gacgaccgta
     6001 ccctcagggg gctagcgaac cggctgagcg ttggccaccg cgtcccggac ggcggccacc
     6061 aggctgcgct cgtcggtgac gtcgatgtcg gtgagccggc gcgtggcgac cgcgatcacg
     6121 tcctcaggct gcggcaccgg gccggaaagc cggggccggt acacctcgac caccaattcg
     6181 ttgtggtggc tgaaaaacgc gaagctgcgc ccgtcgctga tgtggccgaa tccgttggcg
     6241 tgcacgcccg ccgtcacgtc gtcgacgaca aactccttgc cggaacactc actattggcc
     6301 gcgagcgtca tggcatgacc ctaccgcgag aagcgtcggg accactgccg tacgagtcaa
     6361 tatccggccg gacctctgcc ctagactggc ggtcttgaat gtgtgccccg accgacagct
     6421 acccaaggtt tgcaatcatc aaacgtgttt atctcggcgc ggtggcggtc tgctttgtcg
     6481 cgttgagcgc ggtggcgtgc tcggccaacc cggtcgacga cacgccgccg accatcgcgc
     6541 cggcgagtcc ggcggtgtcg ccgcaggtca cgacggctcc tgcaggccaa gtgcgcccgt
     6601 tcccggcgaa ggccaaggaa gccgtgttcg acgcggcgac ggcgtcgctg gtggtgctgg
     6661 gagccgacgg ggtcacgatc atgccggcgg gagacggtgc accccgcaac gtcgcggtgc
     6721 cgagcccgcc aagcgcgctg accggcgacg gtagtggcgc ggtctatctg tcgacccgag
     6781 gcgggtactt ccgcgtcgac gtgcgcaccg gccaggtcgc cacgatgaaa gtggccggcc
     6841 acgagggcac cgacttcacc gcgatcgccc ggcggtccga cggcaggctg gtgctcggca
     6901 gcgccgacgg cgccgtgtac acgctgagct ccgacaccgc ggtcgccgct tcggtcaaga
     6961 tcttcgcacg agtagatgcc cttgtgacac aagggaatac ggcggtggtg ctggaccgcg
     7021 ggcagacgtc ggtgaccacc atcgatccag acggcagcgg agcgcagcac gcattgcgcg
     7081 cgggtgaggg cgccaccacg ctggccgcgg attctgcggg tcgcctgctg gtggccgaca
     7141 ctcgcggcgg ttcgctgctg gtgttcggca ccgacccgct gatgatgcgg cagagctacc
     7201 ccgtccgaga cgcgccatac ggtctcgtcg gcacccgtac cctggcgtgg gtgtcactga
     7261 cggcgaccaa cgtcgtcgtt ggttacgatc tggccaccgg aatacctgtg gagaaagtgc
     7321 gttacccaac cgtgcggcaa cccgatgtgc tggccttcga cgacgccacg aacaccctgt
     7381 acgtggcgtc gtccagcgga gacggcatcc aggtgatcaa cgacgcgggg cggccgcggt
     7441 gacgttggcc ggctgggacg tggacatgtc cgacgactac gaatgggtcc cactccgcct
     7501 gcctcctgac gtgacccggc tgtccgcgtc gacccggctg tcgatcgagg ccgaataccg
     7561 cggctgggag ctcacgcggg tgcggctcta caccgacggc agtcgacgag ttctgttgcg
     7621 gcgcaggaag actgaccggc ccagcgacca gccggggttg taatgctcta ccgcgctctg
     7681 cggcgggtga tgttccttgt gcctgccgag cggatccaca cgtgggtgtt cgcggtgctg
     7741 cgcctggcga cggctgtggc gccggcgcgc cggatgctgc accggctgct ggccccgcat
     7801 gatccggcgc tggccagcac ggtgttcggg gtgcgtttcc ccggtccgct cggtctggcc
     7861 gcgggcttcg acaaggacgg caccgggctg gacacctggg gggcgctggg cttcggcttc
     7921 gccgaggtcg gcaccgttac cgcccacccg cagcccggga acccgtcgcc gcggctgttc
     7981 cggctgccgg ccgagcgggc gctgctcaac cggatgggct tcaacaacca cggggcctgc
     8041 gagctggcgt taaggctggc gcgccacgtc cccgacgtgc cgatcggggt gaacatcggc
     8101 aagaccaagc ttgccgatga tgcggtcggc gattaccgcg caagcacgcg tctcgtcggc
     8161 gcgctcgccg acttcgtcgt cgtcaacgtc agctccccca acacgccggg gctgcgcgac
     8221 cttcaggcgg tggaatcgct gcgcccgatc ctgaccgcgg tgcagtcgga gacgacgacg
     8281 ccggtgctgg tcaaaatcgc gccggatctg tccgacacgg agatcgacga catcgccgac
     8341 ctggccgtcg agctcggctt ggccggcgtc gtcgccacca acaccacggt ccgccaccag
     8401 ctcggcgagg gcggcgtgtc cggagcaccg ctggccccgc ggtcgctgga ggtgttgcgc
     8461 aggctgtacg cgcgggtcgg cgggaagctg gcgttgatca gcgtcggcgg catcgagacc
     8521 gccgacgacg cgtggcagcg gatcaccgcg ggcgcgtcac tgctgcaggg ctacaccggc
     8581 ttcgtctacg gcggcggctt gtggcccaag cacattcacg acggcatcgc gcagcggttg
     8641 cacgcgggag ggttcggttc cctgggtgag gcggtggggt ccgcggcgcg ccgctagctg
     8701 tccttctgct cgtaggtgcc gtggatgacg gcccgggcga tcgcgttcat gaacaggttg
     8761 aacccgaggt aggccgggct ggcgtcctcg tcgagctcca gcttctccac cttgacggcg
     8821 tgcacggcga cgaagtagcg gtgtgttccg tgtccgggcg gcggcgcagc gccgatatag
     8881 cggcgcatgc cggcgtcgtt caccaaagtc actgcgctac cgggcaattc gctaccctcg
     8941 ccgacccccg ccggcaactc ggtgcacgtc gccggcaggt tggccaccgc ccagtgccag
     9001 aacccggacg cggtgggcgc gtcggggtcg tagacggtga cggcgaaact gcgggtctcc
     9061 tcggggaaac cggaccagct cagctgcggc gagacgtcct cgccgccggc gcccatgatg
     9121 ccgctcacct ggggggtctt cagcggctgt ccgtcgctga tgtcggcgga ggtcaacgtg
     9181 aaggtgggca gcttcggcag gaagtcgtaa gggttgtagg gaaacgccac gggtggtcct
     9241 ttctcagggt cagcagttca acagaaagtg ctccagaact cgtgcgccga attccagcgc
     9301 atcgacgggt acccgctcgt cgacgccgtg gaacaacgcc gagaagtcga gctcgggcgg
     9361 aagccgcagc ggggcgaaac caaagcagcg aatccccaag cgcgcaaagt gttttgcgtc
     9421 cgtaccgccg gcgagcatgt agggcactgt gcgggcctct gggtcggtcg ccaaaatcgc
     9481 gtcgttcatc gcgtcgagta gccggccgtc gaaggtggtc tgatacgacg gcaggtcgac
     9541 gatccactcc cgagtgacgt cgggcccgat caactcgtcg acctcccgct cgaacgcaga
     9601 caaccgaccg ggcagcaccc ggcagtccac gaccgcctcc gcggtggccg ggatgacgtt
     9661 ggccttgtag ccggcgttga gcatggtcgg attggcggtg tcgcgaagcg tcgcggcgat
     9721 aaggcggccg atcgagccaa gcttggcgat cgccccgtcc aaatcgggag agttcacgtc
     9781 gaaggtgtag ccggtctcct cggcaacggc ctgcaggaac tgctcgacgg catcggtcat
     9841 caccagggga aattggtgac ggcccagccg cgtcaccgcc tcggccaccg cggtgacggc
     9901 attgtcgtcg tggatcagca tcccgtgccc ggcacggccg tgggcggtca gccgcatcca
     9961 cagcatgcct ttctcggcgg tctcgatgag gtagagacgg cgttcgccgc cgtcgcggcg
    10021 cggcacggtc aatgagaacc cgccgacctc accgatcgcc tcggtgacgc cgtcgaagag
    10081 gtcgggtcgg ttttctacca gccacttgca gccgtacttg ccgccggcct cctcgtcgga
    10141 aacgaaggcg aacaccagat cgcgcggcgg aacgatgccg ctgcgtttga agtgccgggc
    10201 gaccgcgatc gtcatgccga ccatgtcctt catgtcgacc gcaccgcggc cccatacgta
    10261 gccgtcctcg atcgcgccgg agaacgggtg cacgctccag tcggccgcct gggcggggac
    10321 gacgtcgagg tggccgtgga tcagcaacgc gccacggtcg ctgtcctttc cgggcagccg
    10381 cgcgaacacg ttccctcggc cgggcgcgcc ggcctcgatg tactcggtct cgtagccaac
    10441 ctcgtgcaat tgctcggcga cccactgcgc gcactcctgc tcgcctttcg tggtggccgg
    10501 gtcgcctgtg ttggacgtgt cgaagcggat caacgcgctg acgagatcga ccacctcgtc
    10561 gacggcacct gtcacagtcc cctttcctac cactcggcag ctcccccggg ctgcgcccga
    10621 ccccagggat catgggtttg ggaacggcta ctggaatccg atagccttag ctgccacatc
    10681 ggtccgagtg gcggaatggc agacgcgcta gcttgaggtg ctagtgccct attaacgggc
    10741 gtgggggttc aagtcccccc tcggacacaa tttcttagct acacagatag aaaactcgct
    10801 ccgaactcga tcggagcggg ttctctgtat ttgctgctct cgattgggca tcgcgtaatg
    10861 gcatgaacgg gatccaggca ccgagaatgt gagtcaactt c
//
LOCUS       DASWBF010000311         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_759234,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000311 DASWBF010000000
VERSION     DASWBF010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759234"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1008
                     /locus_tag="VF874_11410"
     CDS             430..1008
                     /locus_tag="VF874_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9176648.1"
                     /translation="MTAGTRRGGGAPLDVGTTKAYLQAVVPRVECTEHGVVVAHVPWA
                     RPGAKHTWLFEDTCAWLAAHTAMSVLTVLLRVAWRTVAAVVTRVVADGRATDDLLAGL
                     SRIGIDEIAYRRGHRYLLVTWNHDTGPAVLDRHHDLRDTYRHADDDGAEVAGVRAAPT
                     GGRVAPSVGRSRARTAHDRRVCAGAGGIPALV"
     gene            929..1927
                     /locus_tag="VF874_11415"
     CDS             929..1927
                     /locus_tag="VF874_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9176649.1"
                     /translation="MWADLGRAPRTIDAYARALAEYLLSCENAGVDPIAASRAQVAAF
                     VRELTSRPNRLGANVVSLDSGAGLANATLQQRLVAVRLFYDYLIEEGRRDCNPVGRGR
                     YTPRSRFGGGGHRGLVPALVKLPWIPSERQWFDVLAGFADEPIRNRVMLALAYDAALR
                     REELCSLRTDDLDPAHRMLRVRAETTKTRRERMVPYSAATGALLAQYLTHRGTLSRAR
                     GPLFLSESRRNRAEPLTLWTWSKVVRRIALAANVPQFSTHTTRHLCLTDLARMGWELH
                     AIATFAGHRSTESTMRYIHLSGRDLSDKLNRATADPRAQRIGMLTATATVGAGEQR"
     gene            complement(1870..2538)
                     /locus_tag="VF874_11420"
     CDS             complement(1870..2538)
                     /locus_tag="VF874_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176650.1"
                     /translation="MPGAQRAAVAPPVEDLAHPLRRHVDRGIAEHELRQVQPVEHTQQ
                     MEAVEASDQVGDRDQERAHRGLGRCHPCGQERFRAGAAQARPVIGLPAPVAATGLGAV
                     LQHEPGRVRAGAAIARVGRVVQHRTCVVERLGQQSQPVPQHISLGCAAGAAAAQPHRA
                     ELEQRVQFQLRQLRAPVVAVGAQPERERSPVRLRHGGATNDGGGHRCSPAPTVAVAVS
                     MPIR"
     gene            2843..4261
                     /locus_tag="VF874_11425"
     CDS             2843..4261
                     /locus_tag="VF874_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9176651.1"
                     /translation="MAKAGRWLVAEHPEITEPSQWTRQTCAAWVAAVDRMQVGDHVQR
                     RDCLAARVGTPISPRTKAHVLTCSRTFFHDCQEWEWFPRRFDPARALAVPRSVAALIG
                     TDPRVISDDVWAKLLWAGLNIEPDDLPANTADTYYPMPLIRAVTLTWLFSGLRSDELS
                     RLRVGCVRWQHDGAPIAADARDVPVTDAVCLIDVPVHKTGTAFTKPVDPLLGQAIEAW
                     QAARPAQLATIDRKTSEHVNMLFAVRGQPVARSYINRTIIPALCAKAGVPASDVRGRI
                     TSHRARATIASQLYNAKEPMTLFELQAWLGHRTPATTQHYAKITPNTLAKSYRDAGYF
                     VRNVRTIEVLLDRDAVASGAAAGQPWQHYDLGHGWCSYTFFEQCQHRMACARCDFYTP
                     KGSTQAQLLEAKNNLQRVLAEIPLTDDEQAAVEDDREALAKLLNRLTDTPTPAGPTPR
                     QIGTPPTPTLLPIIGVRHGKSE"
     gene            4274..4869
                     /locus_tag="VF874_11430"
                     /pseudo
     CDS             4274..4869
                     /locus_tag="VF874_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215829.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          783 a         1730 c         1633 g          723 t
ORIGIN      
        1 acacccgagg gtggggctcc agcgtcaatc tcttgtacgg cctaaacggg ccaagccgta
       61 tcgacgtcag cgggcgacgt gcacaccgat tctcatccac ccgcgagcgg gcgcgccagc
      121 gcccattccc atctcttgtg acgttgtagt gggttcgtga gctgggggtt tggggcgcaa
      181 cgccgtgtcc tgcttacgtt ttggactgtt cgaggttcaa gacgaaagaa gggtgtacgg
      241 cgttgcgcaa atccaggata ttcgcggctg ctctcgggga ccacagcatg atcgtcgagg
      301 acgtggcgat cgagacggag acctcgcgtg ggaggcggcc ggtgaccagc gaggtgctgg
      361 tggtgtcggt gcggtgcaag gcttcacagg cgagccggtg ttcgcggtgc cggcgacgct
      421 gcccggggta tgacggcggg gacgcggcgc ggcggtggcg cacccctgga cgtagggacg
      481 acgaaggcgt atctgcaggc cgtggtgccg cgggtggagt gcaccgagca tggggtggtg
      541 gtcgcgcacg tgccgtgggc gcgcccgggc gccaagcaca cctggctgtt cgaggacacc
      601 tgcgcgtggc tggccgcgca taccgcgatg agtgtgctga cggtgctgct gcgggtggcc
      661 tggcgcaccg tggccgccgt cgtgacccgg gtcgtcgccg acggccgcgc caccgacgac
      721 ctgctggccg ggctgtcccg gatcgggatc gatgagatcg cctaccgcag gggccaccgc
      781 tatttattag ttacgtggaa tcacgacacg gggcctgcgg tcctggatcg tcaccacgat
      841 ctgagagata cctaccggca tgcagatgat gatggagcag aagtggccgg tgttcgcgcg
      901 gcaccaactg gcggtcgagt ggctccgagt gtgggccgat ctcgggcgcg caccgcgcac
      961 gatcgacgcg tatgcgcggg cgctggcgga atacctgctc tcgtgtgaga acgccggtgt
     1021 cgacccgatc gcggcgtccc gggcgcaggt tgctgcgttt gttcgtgagc tgaccagccg
     1081 gccgaatcgc cttggcgcga acgtggtttc gctggactcc ggcgcggggt tggcgaacgc
     1141 gacgttgcag cagcgactgg tcgcggtgcg gctgttctac gactacctga tcgaggaagg
     1201 gcggcgggat tgcaacccgg tcggccgcgg ccgttacacc ccgcgcagtc gcttcggcgg
     1261 tggcggtcat cgtgggctgg tgccggcgct ggtgaagctg ccgtggattc cgtccgaacg
     1321 gcagtggttc gacgtgctcg ccgggttcgc cgacgagccg atccggaacc gggtgatgct
     1381 cgcgttggcc tacgacgccg ctctgcggcg ggaggagttg tgcagcctgc gcaccgacga
     1441 cctggatccc gcgcaccgga tgctgcgggt gcgggcggag acgacgaaga cccgccggga
     1501 gcggatggtg ccgtactcgg cggcgaccgg agcgctgctg gcgcagtatc tgacgcatcg
     1561 gggcaccctc agccgggccc gagggccgct gtttctgtcc gagtcgcgac gcaaccgggc
     1621 cgagccgctg acgctgtgga cttggtcgaa ggtggtgcgc cgaatcgcct tggccgccaa
     1681 cgttccacag ttctccacgc acacgacccg gcacctgtgc ctgaccgacc tggcccggat
     1741 gggttgggag ctgcacgcga tcgccacatt cgccggacac cgctcgaccg agtcgacgat
     1801 gcgctacatc cacctgtccg gccgggacct gtcggacaag ctgaaccgcg cgacggccga
     1861 cccgcgtgct caacggatcg gcatgctgac cgcgaccgcc accgttggcg ccggggagca
     1921 gcggtgaccg ccgccatcgt tcgtggcgcc gccgtggcgg agacggaccg gactgcgttc
     1981 acgttcgggt tgcgcgccga cggctacgac cggcgcgcgg agctgacgaa gctggaactg
     2041 gacacgctgc tcgagctcgg cccggtgggg ctgcgccgca gccgcgcccg cggcgcagcc
     2101 cagcgagatg tgttgtggga caggttggct ctgctggccg agccgctcga cgacgcacgt
     2161 gcggtgctgc accacccggc ccacgcgcgc tatcgccgcg ccagcgcgca cgcggccggg
     2221 ctcgtgctgc agcactgcgc cgagtccggt cgcggctact ggggctggca gaccgatgac
     2281 tgggcgggcc tgtgcggctc cagcgcggaa gcgttcctgt ccgcacgggt gacaccgacc
     2341 gagaccacgg tgcgcccgtt cctggtcgcg atcgcctacc tggtcggaag cttcgacggc
     2401 ttccatctgc tgggtatgtt caaccggctg cacctggcgc agctcgtgtt cggcgatgcc
     2461 gcggtcgacg tgtcgacgca gcgggtgggc gagatcctcg acgggtgggg ctaccgcagc
     2521 gcgctgagcg cccggcaccg tctgcgcggc gtgctcgggc aggtcatgct gatcaaccgt
     2581 agcccgcggc tcgccgatct cgacaacgac gccttcaccc ggctacgggc ccatccggcc
     2641 accaaccaac accacggcca gctgctgttc gcgctgcaac gcgcggtcgc ctcactcggc
     2701 cactgcgatc caccgattcg cttgggacac aaccacgcac cgataatcga aggcgccgcc
     2761 gcggcgtggg atcggctggg tcgaacggtg gtacgcgacc tcgacactga ccccccgggt
     2821 ccgcgccacc atccgcaccc agatggccaa ggccgggcgg tggctggtgg ccgagcaccc
     2881 cgagatcacc gaaccgagcc agtggacccg acaaacctgc gcggcctggg tcgccgccgt
     2941 ggaccggatg caggtcggcg accacgtgca gcgccgcgac tgcctcgccg cacgcgtcgg
     3001 caccccgatc tccccacgca ccaaggctca cgtcctgacc tgcagcagga ccttcttcca
     3061 cgactgccag gaatgggagt ggttcccgcg ccgcttcgac cccgcccgcg cgctggcggt
     3121 gccgcgcagc gtcgccgcgc tgatcggcac cgacccgcgg gtcatctccg acgacgtgtg
     3181 ggcgaagctg ctgtgggccg ggctgaacat cgaacccgac gacctgcccg ccaacaccgc
     3241 cgacacctac tacccgatgc cgttgatccg cgcggtcaca ctcacctggc tgttcagcgg
     3301 cctgcgcagc gacgaactgt cccggctgcg ggtcggctgc gtgcgctggc agcacgacgg
     3361 cgcaccgatc gccgccgacg cccgcgacgt gccggtcacc gacgctgtct gcctgatcga
     3421 cgtgcccgtg cacaagaccg ggaccgcgtt caccaaaccc gtcgatccgc tgctcgggca
     3481 ggccatcgag gcctggcaag ccgcgcgacc cgcacagctg gccacgatcg accgcaaaac
     3541 cagcgaacac gtcaacatgc tcttcgccgt ccgcggccag ccggttgccc gcagctacat
     3601 caaccgcacg atcattcccg cgctgtgcgc caaggccggc gtccccgcca gcgacgtgcg
     3661 gggccggatc accagccacc gtgcccgcgc caccatcgcc agccagctct acaacgccaa
     3721 ggagccgatg acgctgttcg agctgcaagc ctggctcggc caccgcaccc cggccaccac
     3781 ccagcactac gcgaaaatca ccccgaacac gctcgcgaag tcctaccgcg acgcgggcta
     3841 cttcgtccgc aacgtgcgca ccatcgaagt tctcctcgat cgcgacgccg tcgcctccgg
     3901 cgccgccgca ggccagccgt ggcagcacta cgacctcgga cacggctggt gcagctacac
     3961 attcttcgaa caatgccaac accgaatggc ttgcgcccgt tgcgattttt acacccccaa
     4021 aggctccacc caagcccaac tgctcgaagc caagaacaac ctgcagcgtg tgctcgccga
     4081 gatcccgctc accgacgacg agcaggccgc cgtcgaagac gaccgcgaag ccctggccaa
     4141 actcctcaac cgtctcaccg acacccccac cccagccggt ccgacacccc gccagatcgg
     4201 caccccaccc acccccaccc tcctgccgat catcggcgtc agacacggaa aatcggaatg
     4261 accttgacaa atcagattcc acagaatgac gatcgtggtc gaccacgaca ccggcacgct
     4321 gatctgggcc gcggccggcc gggacacgaa gacgctgcgc cgcttcttcg acgccctggg
     4381 cgccgaacgc tgcaagctga tcacgcacgt gtccgccgac gccgcggact ggatcgccga
     4441 cgtggtcacc gagcggtgcc cggccgcgat ccgctgtgcg gatccgtttc atgtggtggc
     4501 ctgggccacc gaggcacttg atgccgagcg gcgccgaccc tgaaacgacg cgcgggcgct
     4561 ggcgcgcacc gagcccagac gcggccccgg ccggccccgc aacaacagcg cgcccccgcc
     4621 agctcatgag cgggtccgca agctcaaggg cgcccgctat gcgctgtgga aaaaccccga
     4681 cgacctcacc gaccgccaga ccgccaaact ggcctggatc gccaagaccg acactcgcct
     4741 gtatcgcgcc tatctgctca aagagggcct gcggcatgtg ttttgggtca aaggcgagga
     4801 aggtaagcag gccctggacc ggtggatctc ctgggcgcgc cgttgccgca tcccggtgtt
     4861 cgtggaact
//
LOCUS       DASWBF010000312         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1142966,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000312 DASWBF010000000
VERSION     DASWBF010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142966"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VF874_11435"
     CDS             complement(<1..778)
                     /locus_tag="VF874_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEX9176652.1"
                     /translation="MQDLPGTTSRRRSRPVSRRDALRYATAVSALAGLDAASVGFGTP
                     TAAAAAPQLIDFAARQIPAQHIRAAGYSGVINYVSLSRPGSSFGAKPITRPYAESLTA
                     AGLVIVSNYQYGKPGGTAPSDFTRGFAGGVADARTAWQLHTAAGGGHSAPIFFTVDED
                     INRNAWNTVALQWFRGINAVLGVQRTGVYGGIDVCQWAAADGVIGSSSTPGRRWAWQT
                     RAWSGNQIHPAAVLYQRVVSTASNPGPLVGGLEVDVNDVLA"
     gene            complement(902..1516)
                     /locus_tag="VF874_11440"
                     /pseudo
     CDS             complement(902..1516)
                     /locus_tag="VF874_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          318 a          615 c          594 g          322 t
ORIGIN      
        1 gggccaggac gtcgttgacg tcgacttcga gtccgccgac cagcgggcct ggattcgacg
       61 cggtgctcac cacgcgctgg tagagaacgg cggcggggtg gatctgattg ccagaccagg
      121 ctcgggtctg ccaggcccac cggcggccgg gcgtgctcga actcccgatg acgccatcgg
      181 ctgcggccca ctggcacacg tcgattcctc cgtagacgcc ggtgcgctgc actccaagca
      241 ccgcgttgat tccgcgaaac cactgcagcg cgacggtgtt ccaggcgttg cggttgatgt
      301 cctcgtcgac ggtgaaaaag atcggtgcac tatggccgcc ccctgccgcg gtgtgcagct
      361 gccaggcggt gcgagcgtcg gcgacgccgc cggcgaaccc gcgcgtgaag tccgacggtg
      421 ccgtcccgcc cggcttgccg tattgataat tgctgacgat caccagcccc gcggcggtca
      481 gtgactcggc gtagggccgg gtgatcggct tggcgccaaa cgacgagccg ggacgcgaca
      541 gcgagacgta gttgatcacg ccggaatagc cggcagctcg aatgtgctgc gctggaatct
      601 ggcgcgcggc gaagtcgatc agttggggag cggcggccgc cgccgtgggc gtgccgaaac
      661 ctaccgatgc ggcatccagt ccggccagcg ccgacacggc ggtcgcgtag cgcagcgcgt
      721 cacgccggga aacggggcgt gatcgccgac ggctggtagt ccccggcaaa tcttgcatcg
      781 cgcgaggtta acaatgtgac tgctgttgac ggtgtagcga ctcgctcttg gcggacagct
      841 attgcggggc cgactgcgcg agggagagac cctttgaacc ttcgctgctg acagtacgac
      901 tgttgggatg gccgaacgcc ttgaccagct tggcgtccac ccagtccttg tccggcaggt
      961 ggtcagcgat gttgcgccgc ttgtgcaaag tgcatcgttg gatcagagcc ttggcgccga
     1021 acacctcccg caccgccgcg gcgagcgcct tggcgccgtc gatgaccact agcagcccgt
     1081 cctcgaacgt caagccacgc tcgaccaggt cggccaatcg cgagcgcacc acggtcttgt
     1141 tctcggtgga gccgtcccac agcccgaccg gtttcttcgt gccatcggtg gtgatggcca
     1201 gcgccaccac cacgcaccgc tcggccatgt gctcgccgtc gagcatgagc accttgatgt
     1261 cctcaccggc caggtcgcgg gccatcagct cgcccaacgc agtctcggtc tgttggacga
     1321 accgacgcga aatcgccgag cggctagtcg atttcgcctc ccggtcgacc tgggtaccga
     1381 ccggctcggc ggtgcgcgcg tgccggcggg tggccacccc ggccaacatg cgctccatca
     1441 ccacccgcga cagcaggtcc tcggctgcga agtgcgcgta gctgcccagc ggcacctcat
     1501 gcccatcgac cgcgcggcgc cggtcgtctc ggccgccgat tctgtcgaag actcggcatg
     1561 tcgatcccgg caggtccaca gagatgccaa ctccagtgag aatcggcggc caatccgtca
     1621 gatgccagat tcgttgcgaa acctgccaac tgtactgaat gcggacagct gaataccctg
     1681 cagcgcagcg tgataacgaa tgcgatcgag tttactcgac atacgataaa ctctcggcac
     1741 gctaaatcga gaggcgagct gccttgtgtg ccaggcagtt tcgcaaatta cgtcaccttt
     1801 tcttgctacc ctcaaccagt tatttccggc gacgagggcc ttaccatcg
//
LOCUS       DASWBF010000313         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_811763,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000313 DASWBF010000000
VERSION     DASWBF010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811763"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1905)
                     /locus_tag="VF874_11445"
     CDS             complement(274..1905)
                     /locus_tag="VF874_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEX9176653.1"
                     /translation="MYSAMQDIPLTLTSILRHGTSIHGERVVITATEDGYRQIDYREF
                     GARVAQLANVLHSLGVRPSDRVATFMWNNQEHLEAYFAVPCMGAVLHTLNIRLSDEQI
                     EFIAYDAEDTVVLVDTSLAKQLAAVLPRMETVRTVVAVGDGDTAVLETAGKDVVRYDE
                     LLAAQPTDFAWPDIDERAAAAMCYTSGTTGHPKGVVYGHRSTYLHSASVCSTNGMTLT
                     EGDRALPVVPMFHANAWGISHAALMAGADLVLPDCYAHARRLVQMIEECRPTFAAAVP
                     TIWNDVLHCLENDPGHDISSLRLIVCGGSAVPESLMRTYDEKLGVTLMQLWGMTETSP
                     VASLARPPQGKSADEAWQSRVTQGRPLFGVEMRIVDDDGNVLPNDGKSVGELEVRGPW
                     ITGAYYRDRDAEKFADGWLRTGDVGRIDSLGYVTLTDRSKDVIKSGGEWISSVELENH
                     LMGHPDVLEAAVVGVPDERWQERPLAAVVLKENAAVTAPQLRNYLRDKVVRWWLPERW
                     VFVDEVPRTSVGKFDKKVIRSRYADDVYDVVECTD"
     gene            complement(1923..2296)
                     /locus_tag="VF874_11450"
                     /pseudo
     CDS             complement(1923..2296)
                     /locus_tag="VF874_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215574.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
BASE COUNT          403 a          804 c          689 g          400 t
ORIGIN      
        1 ggcgtgcttc aagctggtcg acgtcgtacc ggcgctgccg aagacccgct cgggcaagat
       61 tctgcgaaaa accatgcgcg gcatcgcgta tggcaaagac gaaccggtgc cgtcgaccat
      121 cgaggacccc aaggtgctcg atcatctccg ctcgatcctg cgtccgtaac tccacgactg
      181 tgcggattca gacgcccacg gcagcgtgtc ccgtacgaaa ccgcacactc gacgcatttt
      241 cacggtccac gccggaatgc caaccacagc aggctaatcc gtgcactcga caacatcgta
      301 cacgtcgtcg gcgtagcggg accggatcac cttcttgtcg aacttgccga cgctggtgcg
      361 cggcacctcg tcgacgaaca cccagcgttc gggtagccac cagcgaacca ccttgtcgcg
      421 caggtaattc cgcaactgcg gtgccgtgac ggctgcgttc tccttgagca ccacggccgc
      481 caacggccgc tcctgccaac gctcgtcggg aacaccgacg accgccgcct ccagcacgtc
      541 ggggtgtccc atgaggtggt tctccaactc gaccgacgaa atccattcgc cgcccgactt
      601 gatgacgtcc ttggagcggt ctgtcaacgt cacgtagccc agcgagtcga tgcgcccgac
      661 gtcaccggtc cgcaaccacc cgtctgcgaa cttctcggcg tcccgatcgc ggtagtaggc
      721 gccggtgatc cacggacccc gaacttccaa ctcccccacc gacttcccgt cgttgggcag
      781 cacgttgccg tcgtcatcga cgatgcgcat ctcgaccccg aacagcgggc ggccctgggt
      841 cacccgggac tgccatgcct cgtcggcgct cttgccctgc ggcggccgcg ccagcgacgc
      901 gacgggcgac gtctcggtca tgccccacaa ctgcatcagc gtcacgccga gcttctcgtc
      961 gtacgtgcgc atcagcgatt ccggcacggc agagccgccg cagacgatca gccgcaatga
     1021 cgagatgtca tggccgggat cgttttccag gcagtgcagc acgtcgttcc agatcgtcgg
     1081 caccgcggcc gcgaacgtcg gccggcactc ctcgatcatc tggacgagcc gccgggcgtg
     1141 ggcgtagcag tccggcaaca ccaggtcggc accggccatc agcgcggcgt gcgaaatgcc
     1201 ccacgcgttg gcgtgaaaca tcggcaccac cggcagcgca cgatcacctt cggttagggt
     1261 catgccgttg gtgctgcaca ccgaggcgct gtgtagatag gtcgagcggt gcccgtacac
     1321 cacgcccttc gggtggccgg tggtgccgct ggtgtaacac attgcggcgg cagcacgttc
     1381 gtcgatgtcg ggccacgcaa aatcggtcgg ctgcgccgcg agcagctcgt cgtagcgcac
     1441 cacgtccttg cccgcggttt ccagcaccgc ggtgtcgccg tcgccgaccg caacgaccgt
     1501 gcgtaccgtc tccatccgcg gcagcactgc tgccaactgc ttggccagcg acgtgtcgac
     1561 gagcaccacc gtgtcttcgg cgtcgtaggc gatgaactcg atctgttcgt cggagagccg
     1621 aatgttcaag gtgtgcaaca ccgcgcccat gcacgggacc gcgaagtacg cctcgaggtg
     1681 ctcctggttg ttccacatga acgtcgcgac ccggtcggaa gggcgaacac cgagactgtg
     1741 caggacgttc gccagctggg cgacgcgggc accgaactcc cggtagtcga tctgacggta
     1801 tccgtcttcg gtggcggtga tcacgacccg ctcgccgtgg atgcttgtgc cgtgacgcag
     1861 gatcgacgtc agcgtcagcg gaatgtcttg catcgcgctg tacaacacgc ggctttctcc
     1921 gatcagtggt gacactcgac cacgtcgtac gcctgatcgg cgtagcgtga ccgaatcagc
     1981 ttcttgtcga acttgccgac gctggtcttg ggcacctcgt cgacgaatgt ccaccgttcc
     2041 ggcagccacc aacgagctac cttgtcgcac agaaagtccc gcaactcatg ggcagtgacg
     2101 ttggcgtcct ccctgaggac gatcgcggcc aacggccgct cctgccagcg atcgtcgggc
     2161 accgcgacga ccgcggcctc gaacacgtcc ggatgcgcca tcaggtggtt ctccagctcc
     2221 accgacgaga ttcactcgcc cccggacttg atcacatcct tcgcgcgatc cgtcagcgtg
     2281 atgaagccgc gcgcgt
//
LOCUS       DASWBF010000314         7371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_423983,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000314 DASWBF010000000
VERSION     DASWBF010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423983"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..542)
                     /locus_tag="VF874_11455"
     CDS             complement(99..542)
                     /locus_tag="VF874_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX9176654.1"
                     /translation="MIVSQPSEQIGELLRAHGLRRTAPRIAVLATLQPVHGHLSVAEI
                     HQRLLDSGHADVPDVATVYRTVTTLVEAGVLHALAVEGGVTTYGVAENPHHHAVCTRC
                     GTIIEVPAQRLSSALEHAIEGSSFTLSDRAGLTLHGLCPDCQAQR"
     gene            700..1812
                     /locus_tag="VF874_11460"
     CDS             700..1812
                     /locus_tag="VF874_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEX9176655.1"
                     /translation="MVADAPQRPVRWLPRARSALTGPEWLRLSSMFGFIALLHLIGWI
                     TLVVFVAPEHYSVGDKALGIGVGMTAYTLGLRHAFDADHIAAIDNTTRKLMGDGQRPL
                     GVGFFFSLGHSTVVFGLAVLLSVGVKAIVGPVEDDSSALHHYTGLIGTSVSGLFLYLI
                     AAINLVILVGIVRVFLRMRGGAYDEAALEHQLNNRGLLNRLFGRFTKSITKSWHMYPL
                     GVLFGLGFDTATEIALLVLAGTSAVAGVPWYAILCLPVLFAAGMSLLDTIDGTFMNFA
                     YGWAFSKPLRKVYYNLTITGLSVAVALLIGSVELLGLLAEQLGLSGGVWDWVAGVDLN
                     VVGFVIVGMFVVTWAVALSVWRFGRIEERWAVAADN"
     gene            1847..3646
                     /gene="ngg"
                     /locus_tag="VF874_11465"
     CDS             1847..3646
                     /gene="ngg"
                     /locus_tag="VF874_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="HEX9176656.1"
                     /translation="MGAGEVGAAGPATSGSGDDHPEAITLGLHDAPPAHLVDVMPANV
                     VLDLGWGRLAFGQTFSDPDELADVLRREGPGRRDICMYAREAHIVVARSPHELFIDPS
                     HTYRLRFSDESLDWSPTGITVRPLETRDDADEMNRVYVRCGMVPARVDVIWDNHVNSD
                     AVTYLVAVRDNDGSVVGTVTGVDHERLFDDPEHGTSLWTLAVDPAAGLPGVGEALTRV
                     LAEHYRSQGRAYLDLSVVHDNAAAIALYEKLGFQRVPVLCIKRKNAINEPLFTSSGPE
                     TVDDLNPYARIIADEALRRGIQVEVLDAETGELRLVHGGRSVITRESLSEFTSAVAMS
                     RCDDKRRTRRIAVETGVTVPRGRLATFGDDDRAVLEEVDDVVVKPTRGEQGKGITVGV
                     DGPDELEAALARAREHCPEVLIEQRAPGDDLRLVVIDGKVVAAAIRRPAEVIGTGRHS
                     VRDLIEAESRRRAAATGGESRIPLDDVTEATVAEAGWSCDDVLPEGIRLRVRRTANLH
                     QGGTIHDVTAKVSPELSRVAVKMADAIGIPVTGIDLLVPDVTEDEYVFIEANERPGLA
                     NHEPQPTAAAFIDFLFPGHPRLPQAWTPESPRP"
     gene            complement(3651..4214)
                     /locus_tag="VF874_11470"
     CDS             complement(3651..4214)
                     /locus_tag="VF874_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9176657.1"
                     /translation="MPSTMLTVDGFPVAVSVAGPEKGPVVTMLGAANQAPAAYDAVCQ
                     RLHTASLRTVVVGPDRRLTPKSVIGILDSLEVKWAMLVGDRAGGEIAWELAASKLDRF
                     IGLLVIDCGHPCVADMTGVVRDGDCPPVEINTTALVSTPAARAVARASQRYVYGDFRL
                     VEMLGRRNAQESTAQLAAEIVLRTSTW"
     gene            complement(4284..4952)
                     /locus_tag="VF874_11475"
     CDS             complement(4284..4952)
                     /locus_tag="VF874_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176658.1"
                     /translation="MLKPVALIAAAAVVAGCATTISGTPTWPGAKLDKVVLTQADFPP
                     GVDYARIVEQPGQPDNFGGPSPMLSVPQGCSNGLTEVIAAHAERGPGSAAKYNVIYDG
                     ARIVMTVLTSPLNLDELAAEAIRCQQFNTYFDRNSEPIPITTTKLPSSRSEQLAYQQT
                     MQLQGVNSSVYMSFENVGTMAVFGIAFPTTQLANGQTSAPKASLPQTFIEIADRQAQR
                     IRDS"
     gene            complement(4946..6436)
                     /locus_tag="VF874_11480"
     CDS             complement(4946..6436)
                     /locus_tag="VF874_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX9176659.1"
                     /translation="MSGALLVAGTSSDAGKSMVVAGLCRLLARKGVRVAPFKAQNMSN
                     NSAVTIEGGEIGRAQAMQARACGLKPSVRFNPILLKPGSDRTSQLVIRGHVAGTVRAG
                     DYVKHRDQHMAVVTAELESLREEFDAVICEGAGSPAEINLRATDLANMGLARAAHIPV
                     VLVGDIDRGGLLAHLFGTLAVLEPADQQLISGFVVNKFRGDPALLEPGLQQLEKLTGR
                     PTYGVLPYVDELWLDTEDSLSVVTNRVVGAPQPPRGTDWLKVAAIRLPRTSNSTDVEA
                     LACEPGVLVRWSIDPAAVADADVVLIPGSKATVADLAWLRKRGLAEAVVAHAEAGKPV
                     LGICGGFQMLCREIDDRVETHAGTVDGLALLDADVEFCPEKTLRSHDKPLSGYEIHHG
                     QVSRCAEELWENVGIRRGAVYGTHWHGLFDNDAFRRDWLAEAARATGRDGFLGADDIS
                     VPARRDAQLDTLADLLTAHLDVDAVCELLESGPPKRPTVVSSLWGC"
     gene            complement(6510..7367)
                     /gene="map"
                     /locus_tag="VF874_11485"
     CDS             complement(6510..7367)
                     /gene="map"
                     /locus_tag="VF874_11485"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX9176660.1"
                     /translation="MPVRSALRSGVLSPQRAVPSSIPRPEYAWKPTVHEGSEPWVQTP
                     EVIENMRVAGRIAAGALAEAGKAVAPDVTTDELDRIAHEYMVDHGAYPSTLGYKGFPK
                     ACCTSLNEIICHGIPDSTVIEDGDIVNIDVTAYIDGVHGDTNATFLAGDVSLEHRLLV
                     ERTREAMMRAIKAVKPGRSLSVIGRVIESYANRFGYNVVRDFTGHGIGTTFHNGLVVL
                     HYDQPSVTTVLEPGMTFTIEPMINLGALDYEIWDDGWTVATKDRKWTAQFEHTLLVTD
                     DGAEILTQL"
BASE COUNT         1133 a         2498 c         2515 g         1225 t
ORIGIN      
        1 gagcgttcca gccacctctg ccgagactgc attttgaaca gccgaacttc gacgaggggc
       61 ctgccggaat gcagtttcgg cgaaaatcag gggagagctc agcgctgcgc ctggcagtcc
      121 gggcacagac cgtgcagcgt cagcccggcc cggtcggaca acgtgaacga gctgccctcg
      181 atcgcgtgct cgagcgccga gctcagccgc tgcgcgggca cctcgatgat cgtcccgcac
      241 ctggtgcaca cggcatggtg gtgcgggttc tcggcgactc cgtaggtcgt aacgccgccc
      301 tcgacggcca gcgcgtgcag cacgcccgcc tcgaccagcg tcgtcaccgt ccggtacacc
      361 gtcgccacat ccgggacgtc ggcgtgcccg gagtcgagca gccgctgatg gatctcggcc
      421 actgacaggt gcccgtgcac cggctgcagc gtcgccagca ccgcgatacg cggcgcggtc
      481 cgccgcagcc cgtgcgcccg cagcaactca ccgatctgct ccgacggttg cgacacgatc
      541 accagatcag tgtcccaccg ccgaccgggg tggacccaag cgccgaaacg gtattccgcg
      601 cgggaaagtg cgagtgaggg ccacggtggc taccgcctcg gcgatgggta gacgatccgc
      661 tcttgcgaat cgcttgcaac aaggcctaac gtcaactccg tggtggccga cgcaccgcaa
      721 cgcccagttc ggtggctccc tcgcgcgcgg tccgcgctca ccggccctga atggttgcgc
      781 ctctcttcga tgttcgggtt catcgcgctg ctgcacctga tcggctggat cacgctcgtg
      841 gtgttcgtcg cacccgagca ctacagcgtc ggcgacaaag ccctgggcat cggcgtcggc
      901 atgaccgcgt acacactcgg cctgcggcac gcgttcgacg ccgaccacat cgccgccatc
      961 gacaacacca cccgcaagct gatgggcgac ggccagcggc cgctgggtgt cgggttcttc
     1021 ttctcgctgg ggcactcgac ggtggtgttc ggcctggccg tgctgttgtc ggtcggggtg
     1081 aaagcgatcg tcgggcccgt cgaggacgac tcgtcggcgt tgcaccacta caccggattg
     1141 atcggcacca gcgtgtcagg cctgttcctg tacctgatcg cggcaatcaa cctggtgatt
     1201 ctcgtcggca tcgtgcgggt gttcctgcgg atgcgcggcg gggcctacga cgaggcggcg
     1261 ctggaacacc agttgaacaa ccgtggcctg ctcaacagat tgttcggccg cttcaccaag
     1321 tcgatcacca agtcctggca catgtatccc cttggcgtgc tgttcgggct cggcttcgac
     1381 accgccaccg agatcgcact gctggtgctc gccggaacca gcgcggtcgc cggcgtgccg
     1441 tggtacgcga tcctgtgcct gccggtgttg ttcgccgccg gcatgagcct gctcgacacc
     1501 atcgacggca cgttcatgaa cttcgcctac ggttgggcgt tctccaaacc gctgcgcaag
     1561 gtgtactaca acctgacgat caccgggctg tcggtggcgg tggcgctgct gatcggcagc
     1621 gtcgaactgc tgggcctgtt ggccgagcag ctcggccttt ccggtggcgt ctgggactgg
     1681 gtcgcgggcg tcgacctcaa cgtcgtcggc ttcgtgatcg tcggcatgtt cgtggtgacc
     1741 tgggcggtgg cgctgtcggt ctggcgcttc ggccgcattg aggagcggtg ggcggtcgcg
     1801 gccgacaact gagcgtgagc tgagcaccgc cggtggggtc gatcgggtgg gggcaggcga
     1861 agtcggagcc gcagggccgg cgacatccgg ctccggcgac gaccatcccg aggccatcac
     1921 gctcgggctg cacgacgcgc cgcccgctca cctggtggac gtcatgccgg ccaacgtggt
     1981 gctcgatttg ggctggggca ggctggcctt cgggcagacg ttctccgacc ccgacgagct
     2041 cgctgacgtg ctgcggcggg agggaccggg ccgccgcgac atctgcatgt acgcccgcga
     2101 ggcccacatc gtcgtcgccc gctcccctca cgaacttttc atcgacccga gccacaccta
     2161 ccggttgcgc ttctccgacg agagcctgga ctggtcgccg accggaatca ccgtgcggcc
     2221 gttggaaact cgcgacgacg ccgacgagat gaaccgggtg tacgtgcgct gcgggatggt
     2281 gcccgcgcgg gtcgacgtga tctgggacaa ccacgtcaac tccgacgcgg tcacgtactt
     2341 ggtggctgtg cgcgacaacg acggatccgt cgtcggcacg gtcaccggcg tcgaccacga
     2401 gcggctcttc gacgatcccg aacacggcac cagcctgtgg acgctggccg tcgaccccgc
     2461 cgcgggcctg ccgggggtgg gcgaggcgct gacccgcgtg ctcgccgagc actaccgctc
     2521 gcaaggccgc gcctacctgg acctgtcggt ggtccacgac aacgccgcgg cgatcgcgct
     2581 ctacgaaaag ctcgggttcc agcgcgtccc ggtgctgtgc atcaagcgca agaacgcgat
     2641 caacgagccg ctattcacgt cgtcggggcc ggagaccgtc gacgacctca acccgtacgc
     2701 ccggatcatc gccgacgagg cgctgcggcg gggcatccag gtcgaggtgc tcgacgccga
     2761 gaccggtgag ctgcgactcg tccacggcgg gcgcagcgtc atcacccgcg agtcgctgtc
     2821 ggagttcacg tcggcggtgg cgatgagccg atgcgacgac aagcgccgca cccggcgcat
     2881 cgccgtcgaa accggtgtca cggtgccgcg cgggcggctg gccacgttcg gcgacgacga
     2941 ccgcgcggtc ctcgaagaag tcgacgacgt cgtcgtcaag cccacgcgcg gcgagcaggg
     3001 caagggcatc acggtcgggg tcgacggtcc cgacgaactc gaggccgctc tcgcgcgggc
     3061 ccgcgagcac tgccccgagg tgctgatcga gcagcgcgca cccggcgacg atcttcgcct
     3121 ggtcgtgatc gacggcaagg tcgtcgcggc ggcgattcgc aggccggcgg aggtcatcgg
     3181 gaccggccgg cacagcgtgc gagacctgat cgaggccgaa agccggcgcc gggcagcggc
     3241 caccggcggc gagtcacgca tcccgctcga cgacgtgacc gaagccaccg tggccgaggc
     3301 cggctggtcc tgcgacgacg tgctgccgga gggtatccga ctacgggtgc gccggaccgc
     3361 gaacctgcac cagggcggca cgattcacga cgtcactgcg aaagtcagcc cggagctgag
     3421 ccgggtggcg gtgaagatgg ccgacgcgat cggcatcccg gtgaccggca tcgacttgct
     3481 cgtgcccgac gtgactgagg acgagtacgt gttcatcgag gcgaacgagc gcccggggct
     3541 ggccaaccac gagcctcagc ccacggccgc cgcgttcatc gactttctgt tcccggggca
     3601 tccgcggctg ccgcaggcgt ggactccgga atcgccgcgg ccgtaaccgt ctaccaggtg
     3661 ctggtgcgca gcacgatctc ggcggccagc tgcgcggtcg actcctgggc gttgcgccgg
     3721 cccagcatct cgaccagccg gaagtcgccg tagacgtagc gctggctggc ccgggcgacc
     3781 gcgcgggcgg ccggtgtgct caccaacgcg gtggtgttga tctccacggg tgggcagtcc
     3841 ccgtcgcgga ccacgccggt catgtcggcg acgcacgggt ggccgcagtc gatcaccagc
     3901 aggccgatga accggtcgag cttactcgcg gccagctccc aggcgatttc gccgccggcc
     3961 cggtcgccga cgagcatcgc ccacttcact tccagtgaat cgaggatgcc gatcaccgac
     4021 ttgggggtca gccgccggtc cggcccgacg acgaccgtgc gcagcgacgc cgtgtgcagt
     4081 cgctggcaaa ccgcgtcata tgccgccggt gcttggttcg cggcgccgag catcgtcacc
     4141 acaggaccct tctcgggacc tgccacactg accgctaccg ggaatccgtc gacggtgagc
     4201 atcgtcgaag gcacctgtcg gacgctacct gcttgagccg cggtaccggg tcgtttttga
     4261 cgattggcag aagatgttca gtttcaactg tcccggatgc gttgggcttg acggtcggcg
     4321 atctcgatga acgtctgcgg cagtgacgct ttcggcgcag aggtttgacc gttagccagt
     4381 tgcgttgtcg ggaaagcgat tccgaacacc gccatggtgc cgacgttctc gaacgacatg
     4441 tagacgctgc tgttaacacc ttgtaactgc atcgtttgtt ggtatgccag ctgttctgac
     4501 cgcgaggagg gcagcttcgt ggtggtgatc gggatcggct cggagttacg gtcgaagtag
     4561 gtgttgaact gttggcagcg gatcgcttcg gctgcgagct cgtcgaggtt gagcggtgag
     4621 gtgagtacgg tcatcacgat tcgtgcgccg tcgtaaatca cgttgtactt cgccgcgctg
     4681 cccgggccgc gctcggcgtg tgcggcgatc acttcagtga gcccgttgga gcatccctgt
     4741 ggcaccgaca acatcggcga cggcccgccg aagttgtccg gctgaccggg ctgctcgacg
     4801 atgcgggcgt agtcgacgcc cggcggaaag tcggcctgcg tcagtaccac cttgtccagc
     4861 ttcgcccccg gccacgtcgg ggtgccgctg atcgtcgtcg cgcagccggc gaccacggca
     4921 gcggcggcga tcagggctac cggcttcagc acccccacag gctactgaca acggtcggtc
     4981 gcttcggcgg gcccgactcc aggagctcgc acaccgcgtc gacgtcgagg tgcgcggtga
     5041 gcaggtcggc cagcgtgtcc agttgcgcgt cgcgtcgggc gggcacgctg atgtcgtccg
     5101 cgccgaggaa cccgtcgcgg cctgtggctc gcgcagcctc ggccagccag tcccggcgga
     5161 acgcgtcgtt gtcgaacagg ccgtgccagt gcgtcccgta caccgcgccg cgacggatgc
     5221 cgacgttttc ccacagctcc tcggcacatc ggctgacctg gccgtggtgg atctcgtagc
     5281 cggacagcgg cttgtcgtgg ctccgcagcg tcttctccgg gcagaactcg acgtcggcgt
     5341 ccagaagcgc gagcccgtcg acagtgcctg cgtgtgtttc gacgcggtcg tcgatctccc
     5401 ggcacagcat ctgaaaaccc ccgcagatgc cgagcaccgg cttgcctgct tcggcgtggg
     5461 cgacgacggc ctcggccagc ccacgcttac gcagccacgc cagatcggcg acggtggcct
     5521 tgctgccggg gatcagcacg acatcggcgt cggcgaccgc ggccgggtcg atgctccagc
     5581 ggaccagcac ccccggctcg cacgccagcg cctcgacgtc ggtggagttc gacgtgcggg
     5641 gcagtcggat cgcggccacc ttcagccagt ccgtgccacg cggaggttgc ggggcgccga
     5701 cgacgcggtt ggtcaccacc gacagcgagt cctcggtgtc cagccacagc tcgtcgacgt
     5761 acggcagcac gccgtaggtg gggcgacccg tgagcttctc gagttgctgc agccccggct
     5821 ccagcagtgc cgggtcgccg cggaacttgt tgaccacgaa gccggagatg agctgttggt
     5881 cggccggctc cagcacggcg agcgtgccga acaggtgcgc gagcaacccg ccgcggtcga
     5941 tgtcaccgac cagcacgacc ggtatgtgcg cggcgcgggc cagtcccatg ttcgccaaat
     6001 cggttgcgcg caagttgatt tcagctggcg accccgcgcc ctcacagatc acggcgtcga
     6061 actcttcgcg cagcgactcg agctcagcgg tcacgacggc catgtgctgg tcgcggtgtt
     6121 tgacgtagtc accggcgcgc acggtgccgg ctacgtggcc gcgaatcacc agctgcgagg
     6181 tgcggtcgct gcccggcttc agcaggatcg ggttgaaccg gacactgggt ttcagcccgc
     6241 acgcccgcgc ttgcatcgcc tgcgctcggc ctatctcgcc gccctcgatg gtgaccgccg
     6301 aattgttgga catgttctgg gccttgaacg gcgccacccg cacgcccttg cgggccaaca
     6361 gccggcacag cccggcgacg accattgact tgccggcgtc ggaactggtg ccggcgacca
     6421 gcagcgcgcc gctcatggat gcgccgaacg tgcgcccact gcgaaaatcc ggccgcaaaa
     6481 tcgcggtgga tgcacgttcg cgagaccgct cacagttggg tcaagatctc cgcgccgtcg
     6541 tcggtcacca gtagcgtgtg ctcgaactgc gcggtccact tgcggtcctt ggtggccacc
     6601 gtccagccgt cgtcccagat ctcgtagtcc agcgcgccga ggttgatcat cggctcgatc
     6661 gtgaacgtca tgccgggctc gagcaccgtg gtgacggacg gctggtcgta gtgcagcacc
     6721 accagcccgt tgtggaacgt agtgccgatg ccgtggccgg tgaagtcgcg aaccacgttg
     6781 tagccgaagc ggtttgcata cgactcgatg actcggccga tcaccgacag cgagcgcccc
     6841 ggtttgacgg ccttgatcgc ccgcatcatc gcctcgcggg tgcgctcgac gagcagccgg
     6901 tgctccaagg agacatcacc ggccaggaac gtggcgttgg tgtcgccgtg cacaccgtcg
     6961 atgtaggcgg tcacgtcgat gttgacgatg tcgccgtcct cgatcaccgt cgagtccggg
     7021 atgccgtggc agatgatctc attcagcgac gtgcagcacg ccttcggaaa acccttgtag
     7081 cccaacgtcg atgggtacgc cccgtggtcg accatgtact cgtgcgcgat gcggtccagt
     7141 tcgtcggtgg tgacatcggg tgcgaccgcc ttgccagcct cggccaacgc gccggcggcg
     7201 atgcggccgg cgacgcgcat gttctcgatc acctcggggg tctgtaccca cggctcgctg
     7261 ccctcgtgca ccgtcggctt ccaggcgtat tcggggcgcg ggatcgacga cggcaccgcg
     7321 cgctgaggtg acagcacgcc ggagcgcagg gcggaacgga caggcatggc c
//
LOCUS       DASWBF010000315         4313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_95972, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000315 DASWBF010000000
VERSION     DASWBF010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95972"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1844
                     /locus_tag="VF874_11490"
     CDS             <1..1844
                     /locus_tag="VF874_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176661.1"
                     /translation="SLLAASSEGGGAALGQVIGMSVVAAIVFAAMLWIGYLHRMRRIT
                     WLVRLADAFGRRFKRPPWVALPTAIFIATLICALFGFIWDVSLHIGKGRDPGPLANPA
                     HYFIFFGLFLLFVAGMVAIVLPYDKPGPAAVKITRNWYAPVGGILMAGCGMYALLGFP
                     LDDIWHRIFGQDVTLWGPTHLMMIGGAGFSTLAVLLLEYEGKRAAGPDVPRDGIGLKF
                     VQYLAFGGILIGLSVFQIEFDFGVPQFRMVFQPMLIAAAAAFGLVAARMILGRGAAIA
                     AALLSIALRGGVALLVGPIFGAPINWFPLYLGPAIVVELLALTPLFKRPIVFGAVSGL
                     GVATVGIWVESLWIDAVYYYSWPPSMWPEALAMAVPVGVLVGVCGAMAGMVLTGQRLP
                     RKAIGISVVVATVLVIGGAVANGLQYQVPKEAVVAIKLTEVSNVDGRKMVQADVRISP
                     LDLVSSHPEWVSILAWQGGLANERGLVVDNLQRVGPGQYRSTQPIPLWGSWKTLLRVH
                     DGKTLAGVPIYLAADPGIGKPAEGIAAQRANAREIFGAPHFQQETTILQRERTSDAPA
                     WLWTAACLVVLACTLTLIGGLTWGAGRINNSETPTEVEAEDRPTVQA"
     gene            1848..2006
                     /locus_tag="VF874_11495"
     CDS             1848..2006
                     /locus_tag="VF874_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176662.1"
                     /translation="MQQHVDYVAHHALLLAIPAFVPAIAVVGVVIWVALRDRRTPDDG
                     TERNETSE"
     gene            2224..2514
                     /locus_tag="VF874_11500"
                     /pseudo
     CDS             2224..2514
                     /locus_tag="VF874_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876191.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2517..3302
                     /locus_tag="VF874_11505"
     CDS             2517..3302
                     /locus_tag="VF874_11505"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9176663.1"
                     /translation="MNNVVVWVLAAVAVAEAVGLVVLWVLLTRTRRDADTLRTRVDAR
                     QQLITGGREAVKTVWQTANLIRKEGLGAAMRSSIEDLADWAEVERPDLARLTPGGNVV
                     ILFSDIEESTALNESMGDRAWVRLLARHDKLVRKCVDEHAGHVVKSQGDGFMVAFAHP
                     AEAVACSIAIQRALGKRPSANTIRVRIGIHMGKSVRRGDDLFGRNVAMAARVAAQADG
                     GEVLISEPVRDAVADEVELGQPRDVELKGFRGTHRLYPVDLAA"
     gene            complement(3278..>4313)
                     /locus_tag="VF874_11510"
     CDS             complement(3278..>4313)
                     /locus_tag="VF874_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9176664.1"
                     /translation="VWTAWTGTAFVLGPLLGGLAVDFLNWRWIFILSAVPVVATFVLS
                     TGLTTDEPHASGSHVDRPGAVLAAAGLAATVFALIEMQRLGLSHPAVATSLGAGLTSL
                     AAFVWWEFRTAHPMLPMHLLGIRNFGVGNLATAFVYAGLSIGMLIVSLFLQEVAGFGA
                     TEAGIATLPLPILSFLLAARVGALAARFGPRLFMASGPLIAAAGFVLMRPEAGHFDFW
                     WQMLPALVLFGLGMTITVTPLTSAVLAAVDPSQSGIGSAINNAVSRIAGLIAVALAGV
                     IAGGALDYGSFRRLTLAAATLFVIGGVVSAIGIRNPAKPTKPVAAEAIAQCRDRPAAP
                     PGGYAARSTG"
BASE COUNT          735 a         1393 c         1430 g          755 t
ORIGIN      
        1 tgtcgcttct tgccgcatcg tccgaagggg gcggtgccgc cctcggccag gtcatcggaa
       61 tgagcgtcgt ggctgcgatc gtctttgccg cgatgctgtg gatcggctat ctgcaccgaa
      121 tgcgacgaat cacgtggctg gtgcgccttg ccgacgcgtt cggccgccga ttcaagcgac
      181 caccctgggt cgcgctgccg acggcgatat tcatcgcgac gctgatctgc gcgctgttcg
      241 gcttcatttg ggacgtcagc ctccacatcg gcaaagggcg agacccgggt ccgctcgcca
      301 atccggcgca ttacttcatc tttttcggcc tcttcctgtt attcgtggcc ggcatggtcg
      361 cgattgtgct gccgtacgac aaaccggggc cggctgcggt caagatcacc cgcaactggt
      421 atgcccccgt cggcgggatc ctgatggccg gctgcgggat gtacgcactg ctcggcttcc
      481 cgcttgacga catctggcac cggatcttcg gccaggacgt cacgttgtgg ggcccgacgc
      541 acctgatgat gatcggcggc gccgggttct ccacgttggc tgtgctgctt ctcgagtacg
      601 agggcaagcg ggcggctggt cccgacgtgc cgcgcgacgg catcggcctg aagttcgtcc
      661 aatacctcgc cttcggcggc atcttgatcg gtctgtcagt gttccagatc gaattcgact
      721 ttggtgtgcc gcaattccgg atggtgttcc agccgatgtt gatcgcggct gcggcggcgt
      781 tcggcttggt ggccgcgcgg atgatcctcg ggcgcggagc cgcgatcgca gcagcgctgt
      841 tgtcgattgc cctacgtggc ggagtggcgt tactcgtcgg accgattttt ggcgcgccga
      901 tcaattggtt tccgctgtat ctcggtcctg cgatcgtcgt tgagctgttg gcattgaccc
      961 cgttgttcaa gcggccgatt gtgttcggtg ccgtgagcgg actcggtgtc gccaccgtcg
     1021 gtatctgggt cgaatcgctg tggatcgacg cggtgtacta ctactcctgg ccgccgagca
     1081 tgtggccgga ggcgctggcg atggccgtgc cggtgggcgt cctcgtcggg gtgtgcggcg
     1141 cgatggccgg catggtgttg accggacagc ggttgccgcg caaggcaatc ggcatcagcg
     1201 tggtagtcgc caccgtgctg gtgatcgggg gcgcggtcgc caacggtctg cagtaccagg
     1261 tgccaaagga ggcggtcgtc gcgatcaagc tgactgaagt gtcaaatgtc gacggccgca
     1321 aaatggtcca ggccgacgtg cggatcagtc cgcttgacct cgtcagctct caccctgagt
     1381 gggtctcgat cctcgcgtgg cagggtggcc tggccaacga acgagggctg gtcgtcgaca
     1441 acttgcaacg cgtcgggccg gggcaatacc gctccacaca accgattccg ttgtggggat
     1501 cgtggaagac actgctgcgt gtccacgatg gcaagacatt ggccggcgtg ccgatctacc
     1561 tcgccgctga tccagggatc ggtaaaccgg ccgagggcat cgctgctcag cgggcgaacg
     1621 ccagagagat cttcggcgca ccgcatttcc aacaagagac caccattctg cagcgagaac
     1681 gcaccagcga tgctcctgcg tggttgtgga cagcagcctg cctggtggtg ttggcctgca
     1741 cgctgacgct gatcggcggt ctgacgtggg gtgccgggcg gatcaacaac agcgagaccc
     1801 cgaccgaagt agaggcggaa gaccgaccca cggtccaggc ttgattcgtg cagcagcacg
     1861 tcgattacgt cgcccaccac gcgctgctgc tcgccatccc cgcgttcgtc ccggcgatcg
     1921 ccgtcgtcgg cgtcgtcatc tgggttgcgc tgcgggacag gcgtactcct gacgacggca
     1981 ctgaaaggaa cgagacctcg gaatgacggc acgagccatc accgcaatgc tgatcgtcct
     2041 cgttcttgtt tccgactgcg ggggatcaaa gaacagtcag ccgagttcgt cagcgccaca
     2101 acagcattcc tcacagcaag cggcacgggc cctcgtcatc gacatcacga tcagcggcgg
     2161 caacgcgact cccaccatcg gcgaattcaa agcgaaagcc catgagccca ttcaactccg
     2221 ggtgaacgcg gacaggagac cttcgtgcgg cttggtgacc cgctgggccg tggctggaat
     2281 ctcttcgggc tcagcaacgc ggaggtgtcg tactttctgt cgtcacatcc gtcggtgttg
     2341 gcgaccctga aggtcgcctg cgtggtggcc gggcacatcg ccgcggtggt cgccgctcac
     2401 gacaaggcgt tgcggctttt gccgcaaggc caccaactga ccgggcaatt ggcgatgatg
     2461 cttgttatgg tcggctacac gttcaccggt ctttacctgc tgttcggcgg ataaccatga
     2521 acaatgtcgt ggtctgggta ctcgctgcag tcgccgtcgc cgaagcggtc ggccttgtgg
     2581 tgctgtgggt gctgctcacc agaacccgcc gggatgccga cacgctgaga actcgcgtcg
     2641 acgcccgcca gcagctcatc accggcggcc gcgaggcggt caagaccgtt tggcagaccg
     2701 ccaatttgat tcgtaaggag ggcttgggtg cggcgatgcg cagctcgatc gaagacctcg
     2761 ccgactgggc tgaggtggag cggcccgatc tcgcaaggct gacgcccggt ggcaacgtcg
     2821 tcatcctgtt ctccgacatc gaggagtcga ccgcgctgaa cgaaagcatg ggcgaccggg
     2881 cctgggtgcg gcttctcgcc cgccacgaca agctcgtccg caagtgtgtc gacgagcacg
     2941 ccggtcacgt cgtcaagagc cagggcgacg ggttcatggt ggccttcgcc caccccgcgg
     3001 aggcggtcgc gtgcagcatc gccatccagc gcgcgttggg taaacgtccg tccgccaata
     3061 ctattcgcgt gcggatcgga attcacatgg gcaagtcggt gcggcgcggg gacgacttgt
     3121 tcggacgcaa cgtcgcgatg gccgcacggg tcgctgccca ggccgacggc ggcgaagtgc
     3181 tgatcagcga gcccgtgcgc gacgcggtgg ccgacgaggt ggagctgggg cagccgcggg
     3241 atgttgagct gaaggggttc cgcggcacac atcggctcta tccggtggac ctggcggcgt
     3301 aaccgcccgg cggtgccgcg ggccggtcgc ggcactgtgc gatcgcctcg gcggcaacgg
     3361 gtttcgtcgg cttggccggg ttgcgaattc cgatcgcgga gaccacacca ccaatcacga
     3421 acagcgtggc tgccgcgagc gtcagccggc ggaagctgcc gtagtccagc gcgccgccgg
     3481 cgatcacccc ggccagcgcg accgcgatca gccccgcgat gcgcgacacc gcgttgttga
     3541 tcgccgaccc gatgccgctc tgcgacgggt cgacagccgc caggacagcc gacgtcagcg
     3601 gcgtcaccgt gatcgtcatc cccaacccga aaagcactag cgcgggcagc atttgccacc
     3661 agaagtcgaa gtgacccgcc tcgggtcgca tcaacacgaa cccggccgcg gcgatcagag
     3721 ggcccgacgc catgaacagc cgcggaccga accgggccgc aagcgcgccg acacgggcgg
     3781 caagcagaaa cgacagaatc ggcagcggca gcgtcgcgat gcctgcctcc gtggcaccga
     3841 acccggccac ctcctgcagg aacagcgaca cgatcagcat cccgatcgac aggcctgcgt
     3901 agacgaatgc cgtcgccaga tttccgacgc cgaagttgcg gatgccgagc agatgcatcg
     3961 gcagcatcgg gtgcgccgtg cggaactccc accacacgaa tgcagccagg cttgtcagcc
     4021 cggcccccag cgacgtggcc acagccgggt gcgaaaggcc aagccgctgc atctctatca
     4081 acgcgaacac cgtcgccgcc aacccggctg cggcgagcac cgcgcccggc cgatcgacgt
     4141 gtgagccact ggcatgcggc tcgtcggtcg tcagacccgt cgacagcacg aacgtcgcga
     4201 ccaccggcac cgccgacagg atgaagatcc accgccagtt caagaagtcg accgccaacc
     4261 cgccgagcag cggacctaac acgaacgccg ttccggtcca agcggtccag acg
//
LOCUS       DASWBF010000316         4444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1015070,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000316 DASWBF010000000
VERSION     DASWBF010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015070"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VF874_11515"
     CDS             complement(<1..953)
                     /locus_tag="VF874_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX9176665.1"
                     /translation="MNEECLKLTTYFGERQRSGDRFLAEAMLDLFGERQIATSIMLRG
                     IAGFGLRHHLRTDQFLSMSEDPPVAVIAVDTKTNIDQLLDSLLTIKKRGLVTLERARL
                     VRDDIGTLELSEELHEATKLTIYVGRKERAYGMPAYIALCDLMYRRQLAGASVFLGVD
                     GTAHGHRERARFFDRNADVPMMIIAVGSGEQIGRVLPELGGLLRRPLITIERVRVCKR
                     DGELLERPHALPGTDEHGLALWQKLMVYTSESARHGGQPIHRALIRALRQRKSSRGAT
                     VLRGIWGFHGDHEPHGDKLFQLSRHVPVVTIVIDTPQNIADS"
     gene            1131..2402
                     /gene="eno"
                     /locus_tag="VF874_11520"
     CDS             1131..2402
                     /gene="eno"
                     /locus_tag="VF874_11520"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018179483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9176666.1"
                     /translation="MPVRFSSMSAVEILDSRGRPTLCVRATLSGGRRVRSGVPSGAST
                     GSREAVELRDHDDRFGGRGVLRAVAHVNGPIAETLTGRSFASLEQVDQTLRGLDGTAD
                     KSRLGANAIVGVSMAAAQAFAAESGEPLWRWLTPSGVIPRLPVPHFNVVNGGAHAPND
                     LDFQEFMIAPLGAPSLPDAVRAGAEVYARLRAELADRHFATGLGDEGGFAPEIDCPED
                     VLALLTKSILDAGYIPGRDGVAIALDPAASEFHRDGRYHVNGESLSSNDLIERYARIV
                     SDFPVWSIEDGLGETDTVGWQKLTAALGERVQLVGDDNFVTNPDLIAFAVRAGIGNAA
                     LIKVNQVGTVSETLAALRVCRDAGYGAMVSHRSGETDDTFIADLAVGSGCGQIKAGAP
                     ARGERVAKYNRLLEIADAKRDMSFGLPPAHH"
     gene            complement(2925..3428)
                     /locus_tag="VF874_11525"
     CDS             complement(2925..3428)
                     /locus_tag="VF874_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX9176667.1"
                     /translation="MTIRPKPDKPAAGQESVWDYPRPPRLERFVGSITVELGGKTIAS
                     TTRAWRVLETSLPPTSYLPRDAFLAGGVLRETSGSSWCEWKGRASYYDLVTEKQVAPK
                     AAWSYLNPSPGFEPIAGAVAVMAALVDRCTVNGEKVVPQPGGFYGGWITSWVVGPFKG
                     IPGSMGW"
     gene            complement(3530..>4444)
                     /locus_tag="VF874_11530"
     CDS             complement(3530..>4444)
                     /locus_tag="VF874_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9176668.1"
                     /translation="ILGFGFVEALYQTVTTITTVGFREVHPLSTVGEFFTIALILVGV
                     GTALYMFGVLLEALIEGHLRQQVGRRRMERQIGRMTGHFIICGWGRVGRASVLYLDAL
                     GKQVVVVDRDPARLQGVDYPTVLGDVTDDSILEAAGIGHAHALIAALNTDADNVYVTL
                     SSRALRPDLVIIARARTEESKSKLLRAGANRAVNPQLIGGRRMAAFALQPHVAEFLDV
                     VMHDETLDYRIEEIKITPASPLVGRCLADTALGSTTGALVLAIRTASGQFITNPAPDT
                     RLDPDTIPIALGTSAQLRSLRSHVDSVR"
BASE COUNT          744 a         1415 c         1501 g          784 t
ORIGIN      
        1 gagtcggcaa tattctgcgg cgtgtcgatg acgatggtga ccaccgggac gtgacggctg
       61 agctgaaaca gcttgtcgcc gtgcggttcg tgatcaccgt ggaagcccca gatgccgcgc
      121 agcacggtcg cgcctcgcga cgacttgcgt tgccgaagtg cccggatcaa cgcacggtgg
      181 atgggttggc cgccgtgccg cgccgactcg gaggtgtaga ccatcaactt ctgccacaag
      241 gccagcccat gctcgtcggt gccgggcagc gcgtgcggtc gctccagcag ttcgccgtca
      301 cgcttgcaga cccggacccg ttcgatcgtg atcaggggac gccgcagcaa tccgccgagc
      361 tcgggcagca cccggccgat ctgctcgccg ctgccgacgg cgataatcat catcggcacg
      421 tcggcgttgc ggtcgaagaa acgggcgcgt tcgcggtgcc cgtgggcggt gccgtcgacg
      481 ccgagaaaca ccgatgcgcc ggcgagctgt cgccgataca taaggtcgca cagagcgata
      541 taggcaggca taccgtaggc gcgttccttg cggccgacgt agatggtcag tttggtggct
      601 tcgtgcagct cttcggacaa ttccagcgtg ccgatgtcgt cgcgcaccag ccgcgcccgc
      661 tccagtgtca ccagaccgcg cttcttgatc gtcaggaggg agtcgagcag ctggtcgata
      721 ttggttttgg tgtccacggc gatgacggca acgggcgggt cctccgacat acttaagaac
      781 tggtcggtgc gcagatggtg tcgcagcccg aaaccggcga tgccgcgcaa catgatgctg
      841 gtggcgattt gtcgttcgcc gaacaggtcg agcatcgcct cggcaagaaa gcggtcgccg
      901 cttcgttgcc gttcaccgaa gtaagttgtc agcttgaggc actcttcgtt cactggtgtt
      961 ctccgatcag ttgcttggcc gcggcagtca tgtgtgtgcg gtgccatatc cggcggtagt
     1021 ctgagcggcg gcggtggggc tcgccataac cgcgcgtccg caatggacgg ccaacggtcc
     1081 ctgtgcagca ggatcggtgc tggccgagcc agcgatgcgg aggaactgaa gtgccggtgc
     1141 ggttttcgtc gatgtcggcc gtcgagatcc tggattctcg tggtcggccg acactgtgtg
     1201 tgcgggcaac gctttccggc gggcgtaggg tgcgctcggg ggtgccgtcc ggggcgtcga
     1261 cgggtagccg tgaggccgtc gagctgcgcg atcacgatga ccgcttcggg ggccggggtg
     1321 tgctgcgggc ggtcgcgcac gtcaacgggc cgatcgccga gaccctgacc ggtcggtcgt
     1381 tcgcctcctt ggagcaggtg gaccagaccc tgcgcgggtt ggacggcact gctgacaagt
     1441 cgcggttggg cgcgaacgcg atcgtgggtg tgtcgatggc ggccgcgcaa gcgttcgcag
     1501 cggagtcggg tgagccgttg tggcggtggc tgaccccgtc cggggtgatc ccgcggctgc
     1561 cggtgccgca cttcaacgtg gtgaacggag gtgcgcacgc acccaacgac ctcgatttcc
     1621 aggagttcat gatcgccccg ctcggggcgc cgtcgctgcc cgatgccgtc cgcgccggcg
     1681 cggaggtgta tgcccgactg cgcgcggagc tggccgatcg ccacttcgcg acgggtttgg
     1741 gcgacgaagg cggcttcgca cccgagattg attgcccgga agatgttttg gcgttgctga
     1801 cgaagtctat tctcgacgca ggatacatac ctggccgtga cggcgtggcg atagcgctgg
     1861 atccggcggc cagcgaattc caccgcgacg gccgctacca cgtgaatggt gaatcgctgt
     1921 ccagcaatga tctgatcgag cggtacgcgc ggatcgtcag cgactttccg gtctggagca
     1981 tcgaggacgg cctgggggag accgatacgg tgggttggca gaagctgacc gcggcattgg
     2041 gtgagcgggt gcaactggtc ggtgacgaca acttcgtgac caaccccgat ttgatcgcgt
     2101 tcgccgtgcg cgcgggcatc ggcaacgccg cgttgatcaa ggtcaaccag gtcggcactg
     2161 tctcggaaac gcttgcggcg cttcgggtgt gccgggacgc cggctacggg gcgatggttt
     2221 cgcatcgctc cggggaaacc gacgatacgt tcatcgcgga cctggcggtc gggtcgggat
     2281 gcggccagat caaggcgggt gccccggcgc gcggcgagcg cgtcgcgaag tacaaccggc
     2341 ttctcgaaat cgccgacgcc aaacgcgata tgtcgttcgg gttgccgcct gcacaccatt
     2401 gacccgccca acccgtcgtc aggaggtcgc gatgaccacg gcgaccaacc taccccgctc
     2461 cgccgctgtc cttgccgttc ccaccgcaat gggtaagtga aaccatcgtg acgagcgccg
     2521 tccggcggcg cgaccgtggc gatcggtgcc cgcgcggcct gaacgaaata gttcactcag
     2581 cacatgagac gccccgccga taagcactgc gtcaccctgc tcgtcgtcgt gcccagctga
     2641 cctgtcgagt aaacaggcgt ttcgtgttct tcgaaaaact ctcgtgcccc tcttggtcgg
     2701 cgcggcgggc aacctagctt cttcgtggaa ggagatctta tgagggattg aacgcgatgg
     2761 cctgaacgaa catggcgaag cgatcacgcc aacgcgggaa gctcgcggcc tggccgggct
     2821 tcaggcggtg ggtcggcggc cactagcggc gcttcccggt taggccgcca tggccggtct
     2881 ttgaccggca ctcgataggc tgccgacccg tcatccggca ccggctacca ccccatggac
     2941 cctgggattc ccttgaaggg accgacgacc cagctcgtta tccagccgcc gtagaagccg
     3001 cccggctgcg gaacgacctt ctcgccgttg acggtgcagc ggtcaacgag cgcggccatc
     3061 acggcgaccg cgccagcgat cggctcgaac cctgggctgg ggttgaggta gctccaggcc
     3121 gctttcggcg caacctgctt ctcggtcacc aggtcgtagt agctcgcccg gcccttccat
     3181 tcgcaccacg acgaccccga ggtctcgcgc agcacccccc cggcgaggaa ggcatcgcgg
     3241 ggtaggtaag acgtcggcgg gaggctggtc tccagcacgc gccatgcgcg ggtggtcgag
     3301 gcgatggtct tgccgcccag ctccacggtg atcgagccga cgaacctctc aagtcgcggc
     3361 gggcgcgggt aatcccacac cgactcctgc ccggcagcag gtttgtccgg ctttggccgg
     3421 atcgtcacac ccaactcctc acggtcactt tcacagcatc gtgccaagca ttaccgacgc
     3481 aagacgctga cggcggcgcg ggcatgccag ggctctgatt ctgacgtcgt catcggaccg
     3541 agtcgacatg gctccgtaag gaacggagtt gagccgaagt cccgagggcg atcgggatcg
     3601 tgtcgggatc caggcgcgtg tccggagccg ggttcgtgat gaactggcca ctcgcggtcc
     3661 ttatggccag caccagcgcc ccggtcgtcg acccgagcgc cgtatcggcc aagcaccgac
     3721 cgaccagagg cgaggcggga gtgatcttga tttcctcgat tcggtaatcg agagtctcgt
     3781 cgtgcatgac gacgtcgagg aactcggcca cgtgaggctg caacgcaaac gccgccatcc
     3841 gccgcccgcc gatcagttgc ggattgacgg cccggttggc gccggcccgc aacaatttgg
     3901 acttcgactc ctccgtgcgg gcacgggcga tgatgacgag atcgggtcgc aacgctcgac
     3961 tggacaaggt cacgtaaacg ttgtccgcat cggtgttcag cgccgcgatc agcgcatggg
     4021 cgtgcccgat gccggcggcc tccaggatgc tgtcgtcggt gacatcgccc agcaccgtcg
     4081 gatagtcgac accctgcagg cgggcggggt ctcggtcgac gacgacgacc tgtttgccga
     4141 gcgcgtcgag atacagcacg ctggcccgac caacgcggcc ccatccgcag atgatgaaat
     4201 gaccagtcat cctgcctatc tgccgctcca tccgccgcct ccccacttgc tggcgtaggt
     4261 gcccctcgat gagcgcttcg aggaggaccc cgaacatgta cagcgcggtt cccacaccca
     4321 cgaggatcag cgcgatcgtg aagaactcgc ccaccgtgga cagcgggtga acctcgcgga
     4381 aacctaccgt cgtgatcgtc gtgaccgtct ggtacagcgc ttcgacaaat ccgaacccga
     4441 ggat
//
LOCUS       DASWBF010000317         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_500499,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000317 DASWBF010000000
VERSION     DASWBF010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500499"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..248)
                     /locus_tag="VF874_11535"
                     /pseudo
     CDS             complement(1..248)
                     /locus_tag="VF874_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731432.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
     gene            465..2189
                     /locus_tag="VF874_11540"
     CDS             465..2189
                     /locus_tag="VF874_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9176669.1"
                     /translation="MARQYRVVDHIVEHLAAIGVSHIFGVDGANIEDLYDAAHFRPDI
                     TAVLAKHEFSAATMADCYSRSGAGLGVVAATSGGGSLNVVPGLGEALASRVPVLALIG
                     QPPTALDGRGSFQDTSGRNGSLNAEALFSAVSLHCQRVTSPADVTPALTRAIAAARCG
                     GPAVLLLPKDIQQGKVNCTAVGDAGPWRNQIGDPHPIARALRRAVGPVTIIAGEQVAR
                     DGVRAELEQLRAVLRARIATVPDAKDTAGTPGLGSSSALGVTGVMGHPGVAEAVAGSG
                     LCLLVGTRLPITARTGLDEALASVPTVSLGSAAPYVPCTHVHTGDLRGSLTLLTLALT
                     GSRRPSGIRVPDAVARTELAPPPFDGPGVRYRDAMAVLDSVLPDGADIVVDAGNTGAA
                     AIHYLPVRRSGRFVVALGMGGMGYSFGAGVGMAFGRARRTVVIAGDGAFFMHGTEIHT
                     ALQYGLPVTFVLFNNNAHAMCVTREQLFYDDLYSYNRFDPANLGAGLAAMFPGLASVD
                     VRDVGDLPDAMRMALAADGPSVVSIECAADEIPPFAPFLGNAVAKTQGLNNHPMTKEN
                     PSDVVARA"
     gene            2170..>2480
                     /locus_tag="VF874_11545"
     CDS             2170..>2480
                     /locus_tag="VF874_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9176670.1"
                     /translation="MSLPALEDITEPIEGLIRIETSSKEQATPLIMEQMRSVYPHDMV
                     FGEYCTVNDYIECPPDELFDYMADTRSLEEWTYSLRGFAPTEEPGLWVAHDKLGSPDG
                     KR"
BASE COUNT          392 a          828 c          838 g          422 t
ORIGIN      
        1 acgtcaacat cgtcgatgct gtcggccccc gcgcacatcg ccgcgatcac cgtggccggt
       61 ttcggcgccg ggttcgctga cccggacttg atccgcggtt tgccgatatg gaaccttctc
      121 ggccagcagc gcaggcaggg cggtctggtc agccagcgtc atcaccggca ccagcccgcg
      181 gcacacgaca cgaggttgcc gtcatcgaac acggccgaat cagccataaa gttgtgcgac
      241 acttgcaccg gaagtgcctt tcgtgggctg gtcagattgt tgtgtggtaa ctccaatcat
      301 cccagctcac agggcacttt cctcacatca acaccccatg gccatcaaca cccgtcggtg
      361 gatcgaggct aagccgcgca cctaccttgc gtcgcccggc ggtgaaccgt agttgctggc
      421 gtgtcgtgta cttgtcaagc ggcgacgcaa ggtaggtgga atacatggca aggcagtacc
      481 gggtggtcga ccacatcgtc gagcatctcg ccgccatcgg ggtcagccac atcttcggcg
      541 tggacggcgc caacatcgaa gacctttacg acgcggcgca tttccggccc gacattaccg
      601 cggtgctggc caagcacgag ttctccgccg cgaccatggc cgactgctac agccggagcg
      661 gcgcgggact cggggtggtc gcggccacct cgggtggcgg ctcgctgaac gtggtgcccg
      721 gcctggggga ggcgctggcc agccgggtgc cggtgctggc attgatcggt cagccgccga
      781 ccgcgctcga cggccgcgga agcttccagg acaccagcgg ccgcaacggt tcgctgaacg
      841 ccgaggcgct gttctccgcg gtgtcgctgc attgccagcg ggtcacctcg cccgccgacg
      901 tcacgcccgc attgacgcgt gcgatcgcgg ccgcccgctg cggcggccct gcggttctat
      961 tgctgcccaa ggacattcag cagggcaagg tcaactgcac cgcggtaggc gatgccggcc
     1021 cgtggcgcaa tcagatcggt gatccgcatc ccatcgctcg ggcgctgcgc cgcgcggtcg
     1081 ggcccgtcac gatcatcgcc ggtgagcagg tggcccgtga cggtgtccgc gccgaactcg
     1141 aacagcttcg tgccgtgctg cgggctcgga tcgccaccgt gcccgacgcc aaggacacgg
     1201 cggggacgcc aggcctcggc tcgtcgtccg cgctgggtgt gacgggcgtg atgggtcacc
     1261 cgggcgtggc cgaggcggtg gccggcagcg ggctgtgcct gctcgtcggc actcggctgc
     1321 cgatcactgc gcgcaccggg ttggacgagg cgctcgcctc ggtgccgacc gtgtcgctgg
     1381 gctcggccgc gccgtacgtt ccatgcaccc acgtgcacac cggcgacctg cgcggctcgt
     1441 tgacgttgct cacgctggcg ctgacgggtt cccgccggcc gtccggaatc cgtgtacccg
     1501 acgcggttgc gcgcaccgaa ctggcgccac cgccgttcga cgggcctggg gttcgctacc
     1561 gcgacgcgat ggccgtgctg gattcggtgc tgcccgacgg ggcggacatc gtcgtcgacg
     1621 cgggcaatac cggcgcggcc gcgatccatt atctaccggt gcgccgcagc ggccgtttcg
     1681 tcgtggcgct cgggatgggt ggcatgggct acagcttcgg cgcgggggtc ggcatggcgt
     1741 ttggccgcgc aaggcggacc gtcgtgatcg ccggcgacgg cgcattcttc atgcacggca
     1801 cggagattca caccgcgctg cagtacgggc tgccggtgac gttcgtgttg ttcaacaaca
     1861 acgcgcacgc gatgtgcgtg acccgcgagc agctgttcta cgacgatctg tacagctaca
     1921 accgctttga tccggccaac ctcggcgccg ggctggccgc gatgttcccc gggttggcat
     1981 cggtcgacgt gcgcgacgtc ggcgacttgc ctgatgcgat gcgcatggcg ctggccgcag
     2041 acgggccatc ggtggtgagc atcgaatgcg cggctgacga gataccgccg ttcgcgccgt
     2101 ttctcggcaa cgccgtcgcg aaaacccaag gcttgaacaa tcatccgatg acaaaggaga
     2161 atcccagcga tgtcgttgcc cgcgcttgaa gacatcaccg agccgatcga aggtctgatc
     2221 cgcatcgaga cgagctccaa ggagcaggcc actccgctga tcatggagca gatgcggtcg
     2281 gtctatccgc acgacatggt gttcggcgag tactgcaccg tcaacgacta catcgagtgc
     2341 ccacccgacg aactgttcga ctacatggcc gacacccgca gtctcgagga gtggacgtac
     2401 agcctgcgtg ggttcgcgcc caccgaggaa cctggtttgt gggtcgcgca cgacaagctt
     2461 ggcagcccgg acggaaagcg
//
LOCUS       DASWBF010000318         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_500511,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000318 DASWBF010000000
VERSION     DASWBF010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500511"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1386
                     /locus_tag="VF874_11550"
     CDS             148..1386
                     /locus_tag="VF874_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9176671.1"
                     /translation="MTVDTDRSGSPSVFDAGLPTIAYEDGQNPDDAHRLIRQARQQAP
                     IAVGPHGPQVLTYDLVRTVLRDPRFRIPHGLGLKAQGITSGPLWDRVIKGLLSLDGAE
                     HHRLRRLVSKAFTPRAVARLRTTIVEVIGDLVDPLIGAGRCDVVADIARQYPIPIICA
                     LLGAPRKDWQLFSDWTDDIFKLFSWNVVNDAPVILAAREALDAYLDDMIAHRRHALTD
                     DLISELIRAEDDGDRLTHDELLMLAAALLTAGTDTTRNQLAAAVQVLCDHPDQWALLA
                     EHPELAPNAVEETMRHSPVVFATLRVAVDDVELGGVLIPAGTLVIANTGAANRDPAVY
                     ADPDRLDITREAPPAMLTFGGGVHYCLGSHLARLELAEALTVITRRMPNPRRTGPAPW
                     KPLTGMSGPTTLPIEFDPDH"
BASE COUNT          273 a          593 c          461 g          238 t
ORIGIN      
        1 gccggaaaac atcagcggaa acgcgtagat tgccgccccc gccgtgcgga gctacgaccc
       61 ctcaccgcgc gcatttacgc gttctgaccg atggtcttgc gccgacgaca tcggatagtc
      121 ggattgttcc gaccggaggg gaccaccatg accgtcgaca ccgatcgctc gggtagcccg
      181 agcgtgtttg atgccgggct gccgaccatc gcgtatgagg acggccagaa ccccgacgac
      241 gcgcaccggc tgatccggca ggcacgacag caggcgccga tcgcggtggg gccgcacggg
      301 ccgcaggtgc tgacctacga cctggtgcgc accgtgctgc gcgatccccg gtttcgcatc
      361 ccgcacgggc tcggtctcaa ggcacaaggc atcacctcag gtccgctgtg ggacagggtc
      421 attaagggcc tgttgagcct cgacggtgcc gaacaccacc ggctgcgccg cctcgtatcc
      481 aaagcgttca ccccccgggc tgtcgcacgg ctgcgcacga cgatcgtcga ggtcatcggc
      541 gatctggtgg atccgctgat tggcgcgggc cgctgcgacg tggtcgccga cattgccagg
      601 caatacccga ttccgatcat ctgcgcgctg ctcggcgcac cccgcaaaga ctggcaactg
      661 ttctcggact ggaccgacga catcttcaag ctcttcagct ggaacgtcgt caacgatgcg
      721 cccgtcatcc tggccgcccg ggaggcgctc gacgcctacc tcgacgacat gatcgctcac
      781 cggcgccacg ccctcaccga cgacctgatc tccgagctga tccgtgccga ggacgacggc
      841 gaccgcctca cgcacgacga actactcatg ctcgccgccg ccctgctcac cgccggcacc
      901 gacaccaccc gcaatcaact ggccgccgcg gtgcaagtcc tgtgcgacca ccccgaccag
      961 tgggcgctgc ttgccgagca tcccgaactc gcaccaaacg cggtcgagga gaccatgcgc
     1021 cactcccccg tcgtcttcgc caccttgcgc gtagccgtcg acgacgtcga gcttggcggg
     1081 gtcctcatcc cggccggcac gctggttatc gccaacaccg gcgccgccaa ccgcgacccc
     1141 gccgtctacg ctgaccccga ccgcctcgac atcacccgcg aggccccgcc agccatgctg
     1201 accttcggcg gcggcgtcca ctactgcctg ggttcccacc tcgccagact cgaactcgcc
     1261 gaagcgttga ccgtcatcac gcgtcgaatg cccaacccgc gccgcaccgg ccccgcaccc
     1321 tggaagccac taaccgggat gagcggcccg acaaccctac ccatcgagtt cgaccccgac
     1381 cactgaacca tggggatcac cccgggaatg taggagccac gacaaccaat gcaggcctaa
     1441 cttcgtggca gcccggcgac ggggcagcag cttcctctcc cgttgaggca actcgcgtcc
     1501 aggcatggca ttgaggcgcc cgttttggga tccgcccgcg ggcactgttg tggtcagcgt
     1561 cgacg
//
LOCUS       DASWBF010000319         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_357897,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000319 DASWBF010000000
VERSION     DASWBF010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357897"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VF874_11555"
     CDS             complement(<1..1365)
                     /locus_tag="VF874_11555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010592525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX9176672.1"
                     /translation="MKLSQNALPQLTIQVPAYDRRAVLVGIAHFGVGGFHRAHQAMYV
                     DRLLNTGLAAGWGICGIGVMPGDKRMRDALLGQDGMYTLIVENPDGTRDPQVIGSIVD
                     FRYAPDDPEAVIGLLAAPTTRIISLTVTEGGYNIRDDDGEFDTDNPDVQHDLRGGEPP
                     VTVFGLVAAAMTRRRDRGLPSPTIVSCDNIVENGNVARRAFTAFAEMSDPALAQWMRE
                     NTRFPNSMVDRITPVTTPDVIEALEADFGVTDAWPVATEPFTSWVLEDDFGDCRPPLE
                     EAGVQLVDDVAPYEMMKLRLLNASHQGRCYFAYLAGYRLVHEAAQDRLIAEFLRRYMD
                     SEATPTLRPVPGIDLDDYKRTLLERFANAYVRDTVARLCAESSDRIPKWLLPVVNDNL
                     RADRPVRLAAATVASWARYAEGVDENGEPIEVVDRLADSLVPIARTQREDPTAFLANR
                     SVFGD"
     gene            complement(1362..2402)
                     /locus_tag="VF874_11560"
     CDS             complement(1362..2402)
                     /locus_tag="VF874_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016693891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX9176673.1"
                     /translation="MRAAVLTHPGSIEVEERRVPEPDVGDVLIRVSDVGVCGSDTHYY
                     REGRIGEYVVESPLILGHEAAGAIVAVGPGVPKERIGQRVSIEPQRPDPSSDETRRGH
                     YNLCPHMRFFATPPVDGALCEYVTIGAAFAHPVPDSMSDTAAALCEPLSVGIAAIGQA
                     AVSGGSRVLVAGAGPIGIMAVQVAQAFGATEIVVTDLDERRRELAGQFGATAALDPRV
                     DDVAHLGVDAFIDASGAAAAVTTGLWAVRPAGRAVLVGMGTPTMELPVQLIQNRELTL
                     TGVFRYANTWETAIELVRLGRVDLDAMVTARYPLEKTAEALESDRVPGNIKSVVEVDE
                     PLGRRARSGGSS"
     gene            2530..>2645
                     /locus_tag="VF874_11565"
     CDS             2530..>2645
                     /locus_tag="VF874_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEX9176674.1"
                     /translation="MTPAPAAAPRASDDLRLALRAATLYYVDGLTQAEVAVRL"
BASE COUNT          434 a          896 c          880 g          435 t
ORIGIN      
        1 atcaccgaac accgagcggt tggccaggaa cgcggtcggg tcctcgcgtt gcgtccgggc
       61 gatcggaacc agcgagtcgg ccagccggtc aacgacctcg atcggctcgc cattctcgtc
      121 gacgccctcc gcatagcgcg cccagctcgc gacagtggcc gcagcaagcc gcaccgggcg
      181 gtcggcgcgc agattgtcgt tgaccacggg aagcagccac ttcgggatcc ggtcagacga
      241 ctcggcgcac agccgggcga ccgtgtcgcg gacgtacgcg ttggcgaacc gttcgagcag
      301 cgtgcgcttg tagtcgtcga ggtcgatgcc cggcaccggc cgcagcgtcg gcgtcgcctc
      361 ggaatccatg tagcggcgca ggaactcggc gatcagccgg tcctgggcag cctcgtgcac
      421 cagccggtac ccggcgagat aggcgaaata acagcggcct tggtggctgg cgttgagcag
      481 gcgcagcttc atcatctcgt acggcgcgac gtcgtcgacc agctgcacgc cggcctcttc
      541 cagcggcggc cggcagtcgc cgaagtcgtc ctcgagcacc cacgacgtga acggctcggt
      601 ggccaccggc catgcgtcgg tgacaccgaa gtctgcctcc agcgcctcga tgacatcggg
      661 tgtggtgaca ggggtgatgc ggtcgaccat cgagttcggg aaccgggtgt tctcgcgcat
      721 ccactgtgcg agagccggat cggacatctc ggcgaacgcg gtgaacgcgc gacgcgcgac
      781 gttgccgttc tcgacgatgt tgtcgcacga gacgatcgtc ggggacggca gcccgcggtc
      841 ccggcgtctg gtcatcgcgg cggcgaccag accgaacacg gtgacgggcg gttcgccgcc
      901 gcgcaagtcg tgctggacgt cggggttgtc cgtgtcgaac tcaccgtcgt cgtcgcggat
      961 gttgtatccg ccctcggtga cggtgagcga gatgatccgg gtggtcggag cggcgagcag
     1021 cccgatcacc gcctcgggat cgtctggagc gtaacggaag tcgacgatcg atccgatgac
     1081 ctgaggatcg cgggtgccgt cggggttctc cactatcaac gtgtacatcc cgtcctggcc
     1141 gagcaacgcg tctcgcatcc gcttgtcgcc cggcatcacc ccgatcccgc agatccccca
     1201 tccggcggcc agccctgtgt tcaggagccg atcgacgtac atcgcctggt gcgcgcggtg
     1261 gaatccgccg accccgaaat gcgcgatccc gaccaggacg gcacgacggt cgtatgcggg
     1321 aacctgaatg gtcaactgcg gcaacgcgtt ctgactgagt ttcacgacga acctccactt
     1381 cttgctcttc gcccaagagg ctcatcgacc tccaccaccg acttgatgtt gcctggcaca
     1441 cggtcggatt cgagggcttc ggcggtcttt tccaacggat accgtgcggt gaccatcgcg
     1501 tcgaggtcca cccgacccaa ccgcaccagt tcgatggcgg tttcccacgt gttcgcatac
     1561 cgaaacacac cggtcagcgt cagctcacgg ttctgaatga gttgcaccgg gagctccatc
     1621 gtcggagttc ccatgccgac taagacggct cgccctgccg gccgcaccgc ccacagtccg
     1681 gtggtgacgg ccgcggctgc gcccgacgcg tcgatgaacg catccacccc taggtgtgcc
     1741 acgtcgtcga cccgggggtc cagcgctgca gtggcgccga attgccccgc gagctcgcgc
     1801 cggcgttcgt cgagatcggt gacgacgatc tcggtggcgc cgaacgcctg ggccacctgg
     1861 accgccatga tgccgatcgg gccggcgccg gcaaccagca cgcgcgaccc acccgagacc
     1921 gcggcctggc cgatcgctgc gataccgaca gacagcggct cacacaacgc cgcggcggta
     1981 tccgacatcg aatcgggcac cgggtgcgcg aacgccgcgc cgatggtgac gtactcgcag
     2041 agcgcaccgt cgacgggtgg ggtggcgaag aaccgcatgt gcgggcacag gttgtagtgg
     2101 ccgcgccggg tttcgtcgct cgacgggtcc gggcgctgcg gctcgatcga aacacgttgc
     2161 ccgatacgtt ctttcggcac acctggcccg accgcgacga tggcgccggc cgcttcgtgg
     2221 cccaggatca gcggtgactc gacgacgtac tcgccgattc gtccttcgcg gtagtagtgc
     2281 gtgtcggagc cgcacacccc gacgtcgctg acccggatca acacgtcgcc gacgtcaggc
     2341 tcagggacgc gacgctcttc gacttcgatg ctgcccggat gcgtgagcac cgctgcgcgc
     2401 atcgtggaag ggagtgttgt ctgcgtcaca tttccatgct agcgtggtga gcatctcagc
     2461 gcacccctga gcatttgctc atcagtgcac gcaggagagg acgcgatggc ggggctggac
     2521 ggacacacga tgacgcccgc cccggctgcc gcgccgaggg catccgacga cttgcggctt
     2581 gccctgcgcg cggcgacgtt gtactacgtc gacggactca cgcaagcgga agtggctgtc
     2641 cgact
//
LOCUS       DASWBF010000320         5297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_550331,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000320 DASWBF010000000
VERSION     DASWBF010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550331"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /gene="ligD"
                     /locus_tag="VF874_11570"
     CDS             <1..1522
                     /gene="ligD"
                     /locus_tag="VF874_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX9176675.1"
                     /translation="APRTWQELDDPDLRHLRFDEVLARVAKDGDLLTPLDADIAGNDR
                     LTKYRSIRDASKTPEPVPISAPKPGNNNRFVIQEHHARRLHYDLRLERDGVLVSWAVP
                     KNLPDTPSVNHLAVHTEDHPLEYLTFEATIPKGEYGAGKMVIWDTGTYEAEKFRVDEV
                     IVTLHGRRISGRYALIQTKGDQWLAHRMKEQESPTVRDLAPMLATLGSVERLTHTDWA
                     FEGKFDGYRLLLEADHGRMCLQSRSGRDVTGEYPRLQGVVDALAEHHAILDGEVVALD
                     NSGVPSFSEMQNRARATRIEFWAFDVLQLDGRSLLRARYRDRRRVLEALAAERDELIV
                     PELLSGDGPDASAYSRKRGWEGVVAKRWDSTYQPGRRSRSWIKDKNWNTQEVVIGGWR
                     EGAGGRTSQIGALMMGIPEKGGLRFVGRVGTGFTEKELGRLKDILAPLRTDESPFTAP
                     LSRQDAKGVTFVKPELVGEVRYGEWTGDGRLRHPSWRGLRPDKTPADVEVEQPVST"
     gene            complement(1632..2504)
                     /locus_tag="VF874_11575"
     CDS             complement(1632..2504)
                     /locus_tag="VF874_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9176676.1"
                     /translation="MRFTYAEAMTDPTFYIPLAKAAEAAGYDGMTIPDSIAYPLESDS
                     TYPYTPDGSREFLDGKAFIEAFVMAGALCAVTTSLRFNFFVLKLPIRPPALVAKQAGS
                     LAALFGNRLGLGVGTSPWPEDYELMGVPFAKRGERMDECIEIIRGLTSGDYFEFHGEF
                     YDIPKTRMTPAPTQPIPILIGGHADAALRRAARCDGWMHGGGPPEELDRLLLRLRQFR
                     SETDRSGPFEIHVISADGFTVDGVKRLEDKGVTDVIVGFRVPYIMGPDTEALGKKIHK
                     LEKFAERVIAKVTG"
     gene            complement(2780..3097)
                     /locus_tag="VF874_11580"
                     /pseudo
     CDS             complement(2780..3097)
                     /locus_tag="VF874_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
     gene            complement(3102..4132)
                     /locus_tag="VF874_11585"
                     /pseudo
     CDS             complement(3102..4132)
                     /locus_tag="VF874_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
     gene            4260..5165
                     /locus_tag="VF874_11590"
     CDS             4260..5165
                     /locus_tag="VF874_11590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9176677.1"
                     /translation="MTPLQRYIAEEIATDHVDGLLSRREALRRLALLGLSAGAASALI
                     AACGAGGKRDSTSATTGTEAVTSAPPGMDRALPTSPVTWAGPNGELQGAWAAAAEPRG
                     GVLVIHENKGLNDWVRSVAGRFAGVGYSALAIDLLSEEGGTGKFTDPAEATAALGKVS
                     PDRFVADLKSGVGELQRRVAGAKVAAVGFCFGGGMVWQLLASGEPSLAAAVPFYGPLP
                     DNPDFSGSKAAVLAIYGALDARVNASKDAAVAALQRAGLVHDVYVAPDADHAFFNDTG
                     SRYNPAAAAEAWGRVLDWFGRYVTS"
     gene            complement(5150..>5297)
                     /locus_tag="VF874_11595"
     CDS             complement(5150..>5297)
                     /locus_tag="VF874_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4873 domain-containing protein"
                     /protein_id="HEX9176678.1"
                     /translation="VFGAQVELPKRPVTITTDTLTAQAKITEQTPWGSYSIAGIGAPP
                     YEVT"
BASE COUNT          860 a         1729 c         1824 g          884 t
ORIGIN      
        1 ggcgccgcgc acgtggcagg aactcgacga ccccgatctg cggcatctgc gcttcgacga
       61 ggtgctcgca cgcgtcgcca aggacggcga cctgttgaca ccgttggacg ccgacatcgc
      121 cggcaacgac cggctgacga agtaccgcag catacgtgac gcgtcgaaaa cacctgagcc
      181 ggtgcctatt tcggcgccca agccgggaaa caacaaccgg ttcgtcatcc aggaacacca
      241 cgcccgtcgc ctgcactacg acctgcggct cgagcgcgac ggcgtgctcg tcagctgggc
      301 ggtgcccaag aacctgcccg acaccccgtc ggtgaaccac ctcgccgtgc acaccgagga
      361 ccatccgctg gagtacctga cgttcgaggc gacgatcccc aaaggggagt acggcgccgg
      421 caagatggtt atctgggaca ccggcaccta cgaggccgag aagttccgcg tcgacgaagt
      481 catcgtgaca cttcacggtc ggcgaatctc ggggcgctac gcgctgattc agaccaaggg
      541 cgaccaatgg ctcgcacatc ggatgaagga gcaggagtcg ccgaccgtca gggacttggc
      601 gccgatgttg gccacgctgg gctcggtcga gcggctgacg cacaccgact gggcgttcga
      661 gggcaagttc gacgggtacc ggctgctgct ggaagccgac cacggacgga tgtgcctgca
      721 gtcacgcagc ggccgcgacg tcaccggaga atatccgagg ctgcaagggg tggtggacgc
      781 gctggccgaa caccacgcga ttctcgacgg cgaagtcgtc gcactcgaca actccggtgt
      841 gccgagcttc agcgagatgc agaaccgggc gcgcgcgacc aggatcgagt tctgggcgtt
      901 cgacgtgctt cagctcgacg gcaggtcgct gctacgggct cgctatcgcg accggaggcg
      961 agtgctggag gcattggccg ccgaacgcga cgagctgatc gtccccgagc tgctgtccgg
     1021 tgacgggccc gacgcgtcgg cgtactcccg caagcgtggc tgggaggggg tggtggctaa
     1081 gcggtgggac tccacctatc agccggggcg gcgctcgcgg tcgtggatca aggacaagaa
     1141 ctggaacacc caggaggtgg tgatcggcgg ctggcgggaa ggcgctgggg ggcggacaag
     1201 tcagatcggc gcgttgatga tgggcatccc cgagaagggc gggttgcggt tcgtcggccg
     1261 cgtcggtacc ggcttcaccg agaaggagct tggtcggctc aaggacatcc tcgctccgct
     1321 gcggactgac gaatctcctt tcaccgcacc actttcccgg caggatgcca aaggcgtgac
     1381 gttcgtgaag ccggagctgg tcggcgaggt ccgctacggc gaatggaccg gcgacggacg
     1441 cctgcgccac cccagctggc gtggcctgcg acccgacaag accccggcgg acgtcgaagt
     1501 cgagcagccg gtctcgacgt agtctccgag tcccgtccag ataccttgca attccccgag
     1561 actcaaacct ctgcggccct cgcggcgtgt cgccgcagac gtttcagtgt cggcgacggc
     1621 ggcgacggcg gctagccggt cactttggcg atgacgcgct cggcgaactt ctcgagctta
     1681 tggatcttct tgcccagtgc ctcggtgtca gggcccatga tgtagggcac ccggaagccg
     1741 acgatcacgt cggtgacgcc cttgtcctcg agccgcttga ccccgtcgac cgtgaacccg
     1801 tcagccgaga tgacgtgaat ctcgaacggt ccggaccgat ccgtttcaga gcgaaactgc
     1861 ctgagccgca acaggagccg atccagttcc tccggagggc cgccgccgtg catccagccg
     1921 tcgcagcggg cggcgcggcg cagcgcggcg tcggcgtgcc cgccgatcag gatcgggatc
     1981 ggctgcgtcg gtgccggcgt catcctggtc ttggggatgt cgtagaactc gccgtggaac
     2041 tcgaagtagt cgccggacgt cagccctcgg atgatctcga tgcattcgtc catccgctcg
     2101 ccgcgcttgg cgaacgggac gcccatcaac tcgtagtcct ccggccatgg gctggtgccg
     2161 acgccgagcc ccagccggtt gccgaacagc gcggccagcg agccggcctg cttggcgacc
     2221 agcgccggcg ggcggatcgg caacttgagc acgaagaagt tgaaccgcag cgacgtggtg
     2281 accgcgcaca gcgcgccggc catcacgaag gcttcgatga acgccttgcc gtcaagaaac
     2341 tcgcgactgc cgtcgggtgt gtacgggtag gtggagtccg actcgagcgg gtaggcgatg
     2401 ctgtccggga tcgtcattcc gtcgtagccg gcggcttcgg cggccttggc cagcgggatg
     2461 tagaacgtgg ggtcggtcat ggcctccgcg tacgtgaagc gcaccgcgtc ctcctcacga
     2521 cagcatccgg tgccgttgcc agtgtgtgag cgtagcgtcg gttcaccaag ccaacccgag
     2581 acttcatcgg ccaagctaag ggcatgatca tcggcgacgc gaatcacccg ccgcgatgac
     2641 gagcccgtcg caccgcgcca atcaggggac tccggccgcc gagcgagccg agttggcgcg
     2701 catgcgcgcc gaagaactca aggtgcgtcg cggtgggctg atcaagccgg gccgccgacg
     2761 cctcgatcgc ttccgccgtc gacgggtaca ccggcacgta tcgcgtgatg ccgttgcgct
     2821 cgatgatttt tcggccttcc gggtgcgagc acaccagcag gatcgggacg tccggccaca
     2881 tttcgacgtg ccagcgcgcg ctggtgaaca ccgcccacgc cgacggagag gggacggtca
     2941 acgggctgac gtcgatcacc accgcccgcg gttcgtcgag cgcggccttg attatctgat
     3001 cacgcaacgg gagatacgtt gtgctgttga ggatcccgtc gacattcagc agccgtgttc
     3061 ccgcggtctc cgcgaccgag acgaccaatt tctccaggcg atcagccggc accgcgctca
     3121 cctgtttcgc tgtcggcctc catgaggcgt tcccccagca ccttcacggc gcgttcggtt
     3181 tcgtcggcga cggcgccgta cctttcgcca aggaccccgg ggccgaagtt gtcggccatc
     3241 ctgcgcgcca gtttggcctt ctcctgcagg ctgcgcagcg cgatccacag cgcgctgtcg
     3301 acgtcctcgt cgcgcgcccg aagcagtgcc tctcccgtcc aggcatgccc tgtctggcac
     3361 cggaagttgc ggaattgccc tccgcgaccg attgcaggga accgttgcag tccgggcaca
     3421 cgtaccccga cggcggaccg aggtcctcgg tgtcgaagga cgtcgagaac cgcttcgcgc
     3481 gcgcgatgcg attttccaac tccatgttgg cgtcgcgtcc caccggctca tcctcgggtg
     3541 ggcgatcgac aagctgcatg cgcagctgac ccgctcctgc cgcggtgact tggtgatcga
     3601 ccacgcccgc ttcgacggcg ttgagcggca tcgccgcgta cagcgcgtcg ttgggatgtt
     3661 ggacgagggt gacgccaccg cgggagcgga tcgccgccgc tccgagcact ccatcatcga
     3721 gcaccccgga cagcagcaac ccgatcgctc gcgggccgta ggccagtgcc gcggaccgga
     3781 acagggcgtt gatggccggc cgatagccgt tctccgtagg tccggcggac aacacggcgc
     3841 ggtggtcttc gatcagaagg tgatggtccg gcacggcgac gtggatctgt cccggtttca
     3901 gtcgcatgcc gtcaaccgca gaacacgcgg gcaggggtcc actgcggtcg agaatcttcg
     3961 ccagcacgct gggtgcgcca ggtggcatgt gcaagacgac gacgaccgca aaaggaaagt
     4021 cgcacggcat gcctgcggcc atgttggtga gcgcctcgac cccgccggct gaggcgccga
     4081 ccgctacgat tccgcgcggt tcgttgttcg cactgttcgt ctcgggcccc atttcggtgc
     4141 gggtacccag cgatctgttg ggcaacccgg ttgtggtccc acgagaactg atcccgaacg
     4201 cgtggcgcct cggagactac gcttcttcag cagcttctcc tcggcgcata ctcgcgtatg
     4261 tgactccgct gcagcgctat atcgccgaag aaatcgccac cgatcacgtc gacgggctgc
     4321 tgtcacgacg ggaagccctg cgccgcctcg cgcttctcgg cctgagcgcg ggtgccgcca
     4381 gcgcgttgat cgcggcctgc ggtgcgggtg gcaagaggga ctcgacgagc gcgaccaccg
     4441 gaacggaggc ggtcacctcg gcgccgcccg ggatggatcg ggcattgccc acttcgcccg
     4501 tgacatgggc gggtccgaac ggcgagctgc agggcgcgtg ggctgctgcg gccgagcctc
     4561 gcggcggcgt gttggtcatc cacgagaaca aggggctcaa cgactgggtc cgttcggtgg
     4621 ccggtcggtt cgccggcgtc gggtattcgg cgttggcgat cgatctgctg tccgaggaag
     4681 gcgggacagg caagttcacg gatccggcgg aggcgaccgc agcgctgggc aaggtgtccc
     4741 cggaccgatt cgtcgccgac ctcaagtcgg gtgtcggcga gctgcagcga cgggttgcgg
     4801 gggccaaggt tgccgccgtc gggttctgct tcggcggcgg gatggtgtgg caactgctgg
     4861 cgtcgggtga gccgtcgttg gctgccgcgg tgccgttcta cggaccgctg cccgacaatc
     4921 cggacttctc cggctccaag gcggcggtgc tggccatcta cggtgccctg gacgctcggg
     4981 tgaacgcgtc gaaggacgct gcggtcgcgg cgcttcagcg tgcggggctg gttcacgacg
     5041 tgtacgtcgc cccggatgcc gaccacgcgt tcttcaacga caccggctcg cggtacaacc
     5101 cggccgctgc ggccgaagcg tgggggcgag tgctcgactg gttcggtcgt tacgtgactt
     5161 cgtaaggcgg agctccgatt ccggcgatcg agtagctgcc ccacggcgtc tgctcggtga
     5221 tcttggcctg tgctgtgagc gtgtcggtgg tgatcgtgac gggccgtttc ggcaactcga
     5281 cttgtgcgcc gaaaacc
//
LOCUS       DASWBF010000321        15704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_760260,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000321 DASWBF010000000
VERSION     DASWBF010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15704
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760260"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF874_11600"
     CDS             complement(<1..261)
                     /locus_tag="VF874_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEX9176679.1"
                     /translation="MPKLQLVFDPAADALLDDNPMALLIGMLLDQQVPMETAFAGPKK
                     IADRIGGIDARLIADYDPEKFAALCSERPAVHRFPGSMAKRIQ"
     gene            486..1615
                     /locus_tag="VF874_11605"
                     /pseudo
     CDS             486..1615
                     /locus_tag="VF874_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1626..2939
                     /locus_tag="VF874_11610"
     CDS             1626..2939
                     /locus_tag="VF874_11610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9176680.1"
                     /translation="MALFPSPAEIAAQTPPARDRAIDVIRIVSLVAVVFGHTIMAVST
                     VRDDVFVWGNLLSSVPALGALTWVLQIMPLFFFAGTAACVESWAPGHSWGNWLMQRCT
                     RLFRPVFFYLAFWGVALAVLRFVLPEHVYEPVAGTSIQLLWFLGAYVLVLAAIPLLYR
                     ITTTKRLALGVLAVYGLVVAVDAVRLNVDGLSVLGYLNVVVWLIPGMFGVAYRRGLLC
                     GLAAKAVAALMLVVNVLLVWLGPYELSLVGYQEQRLPNMTPPSALLAGHAVMMCAVAI
                     AAAPAIARWAQRPRVWWLVAIGNSGAMTLYLWHMPALLGMHLVFDALGLPRYDVTAPD
                     FVALSVVQLVLMAVMVGVLFVALRPLENNPLPGWDGGFVARPGARSAAVGSLLCATGT
                     ATLVSVRWGLKDDGLICVAVLLVALIAARALATDAKVPAAAPVSG"
     gene            complement(2936..3109)
                     /locus_tag="VF874_11615"
     CDS             complement(2936..3109)
                     /locus_tag="VF874_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HEX9176681.1"
                     /translation="MAADGTPEDDTKRKFREALERKNAKASGGEQHKDTGAKQPRTHG
                     PAEHRREFRRKSG"
     gene            complement(3128..3571)
                     /locus_tag="VF874_11620"
     CDS             complement(3128..3571)
                     /locus_tag="VF874_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX9176682.1"
                     /translation="MAPQAFDDKLLAVISGNSLGVLATIKRDGRPQLSNVSYHFDSRT
                     AVIEVSITEPRAKTRNLRRDPRASILVTSDDGWSYAVAEGTAELSPPAADPHDDTVEA
                     LIALYRNIAGEHPDWDDYRRAMVTDRRVLMRLPISHVYGMPPGLR"
     gene            3611..4129
                     /locus_tag="VF874_11625"
     CDS             3611..4129
                     /locus_tag="VF874_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX9176683.1"
                     /translation="MARYAAFLRGVNVGGVNLKMADVAEALTEAGFSDVRTILASGNV
                     LLESRSKVAAVRTTAEAALREAFGYDAWVLAYDVKTVQAIVDAYPFEREVPDHHSYVT
                     FASDPEVLDELAALEPGPDERIERGKGVIYWQVPRSKSLQSAMGKTMGKKRYKSSTTT
                     RNLRTLDRVLRR"
     gene            4141..4974
                     /locus_tag="VF874_11630"
     CDS             4141..4974
                     /locus_tag="VF874_11630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9176684.1"
                     /translation="MTSSNGHRADVTTLTGVSETALLTLNVRAREARRPDAIIEDPMA
                     IKLVDSIDYDFAKFGPSNRQDVAVRALAFDHNARRYLAAHPHATVVALAEGLQTSFWR
                     LDEPGDQFHWLTVDLPRIIELRRRLLPRSPRISERAQSALDFSWMDDVDPAQGVFVTA
                     EGLLMYLQPDEAMGLIRECARRFAGGQMMFDLPPRWAAALARHGFRASLRYKVPPMPF
                     SLSLAEIADLVNTVPGIRAVHDMPLPRGRGPVFNLLLWTIQRLPLFDPVRPAITLLEF
                     G"
     gene            complement(5136..5507)
                     /locus_tag="VF874_11635"
     CDS             complement(5136..5507)
                     /locus_tag="VF874_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9176685.1"
                     /translation="MSELADWVRLDSKAGTPLFDQLRTQIIDGIRDGRLAAGTRLPTV
                     RALAGQLGLAVNTVARTYRELETAGMVETRGRAGTFVCGVDPVDARMVAAARIYVDTA
                     RALGVGSADAVRFVEAAFGPR"
     gene            5544..6257
                     /locus_tag="VF874_11640"
     CDS             5544..6257
                     /locus_tag="VF874_11640"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX9176686.1"
                     /translation="MRVTVLSGAGMSAESGVATFRDAQTGLWETFDPEELASPAAWLR
                     QPDVVWAWYLWRHHLVRSVEPNPGHYALADWQDRADVRVVTQNIDNLHERAGSQHVAH
                     LHGSLFDFRCSRCGERYHGELPDMPEPVGAADPPLCACGGHIRPGVVWFGEALPDEPW
                     QQALDAVAGADVVVVVGTSGIVYPAAGLPSAALAQGIPVVEVNPDDTPLSRSATVTVR
                     DTAANALPRLLDRLHELLG"
     gene            complement(6261..6896)
                     /locus_tag="VF874_11645"
     CDS             complement(6261..6896)
                     /locus_tag="VF874_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9176687.1"
                     /translation="MSSNRVDNPFFARMWTVLSRYEPESLRELRRENLASLSGRVLEV
                     GAGTGSNFVHYPTSVEEVVAVEPEPRLNAIAQRTAATAPVPVTVMDRAVEDIEPGEPF
                     DAVVCSLVLCSVEDQEAVLRQLFWMLRPGGELRYLEHVASSGARGRLQRLADATIWPR
                     LAGNCHTHRDTETAIVNAGFVQQTARHDWTLPAWVPVPVSEHTLGRAVRPA"
     gene            complement(6889..7590)
                     /locus_tag="VF874_11650"
     CDS             complement(6889..7590)
                     /locus_tag="VF874_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9176688.1"
                     /translation="MSAPVRSSDRRRRAPRGSGDQLRDEILDAATDLLLETGHAKAVS
                     IRSVAQRVGVTPPSIYLHFTDKDALLDAVCARYFEKLNEEMQRAAVGQTTTLDVLRAQ
                     GQAYVRFALQTPELYRIATMSEGRPGSDVDVTLNSSAFVHMRASVQALIDEGIYVDGD
                     ATQTALEMWTAAHGVAAMLIAKPYLPWGDAETFTDRVLQAVCCGQIVSGIIGYDTCPQ
                     DAINAIMKLRGPDHE"
     gene            complement(7571..9868)
                     /locus_tag="VF874_11655"
     CDS             complement(7571..9868)
                     /locus_tag="VF874_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9176689.1"
                     /translation="MLTGIARLAIAAPRRIIGLALLVLVGTAVFGIPVTKSLSAGGFQ
                     DPTSQSAEAKKMLSETFDQGDLTLVISVTCEPGAQSPEARQTGTDIARQLSESPFIAQ
                     VNSAWTSPPSVAPALVSKDGKTGLIVAGISGTESEAQQRAKTLTSQVVHDRGGVTVKA
                     GGEAMIYVQINGQSEKDLLLMESIAIPLSFLVLVWVFGGLLAAALPLAVGGFAILGSM
                     AVLHAITFATDVSIFALNLTVAMGLALAIDYTLLIISRYRDEIADGAGRDEALIRTMA
                     TAGRTVLFSAMTVALSMVAMVLFPMYFLKSFAYAGVAVVAFAAAAAIIVTPAAIALLG
                     DRLDSLDVRKLGRRLLHRPEPVKKPVEQTFWYRSTKFVMRHAIPVGIAIVALLMVLGA
                     PFLGAKWGFPDDRVLSQSASARQIGDSLRSDFAVDSATNVIAVIPDMSGVTPEELSRY
                     AADLSKVPDVSSVSAPDGSYVRGALAGPPSAATGVKDRRAFLTIGSTEPLFTPASEDQ
                     LDRLHAVSTPAGHDVLLTGTAQVNRDSSQAVTSRLPTVLAVIAAITFVLLFMLTGSVV
                     LPLKALLLNILSLSAAFGALVWIFQDGHLGAFGTTSTGTLVANMPVLLFCIAFGLSMD
                     YEVFLISRIREYWLQSGRTRADRSSLAPRVRNDESVALGLARTGRVVTAAALLMSISF
                     AALIAAQVAFMRMFGVGLTLAVLVDATLVRMLLVPAFMHVLGRANWWAPKPLARLHDR
                     FGISEARGGSEPAMTETGQRVGTRS"
     gene            10292..10423
                     /locus_tag="VF874_11660"
     CDS             10292..10423
                     /locus_tag="VF874_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176690.1"
                     /translation="MWIDDTSADVIKVDFDALYHGDVLVEGDTSEQLDDWHPLAPAV"
     gene            complement(10484..11482)
                     /locus_tag="VF874_11665"
     CDS             complement(10484..11482)
                     /locus_tag="VF874_11665"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX9176691.1"
                     /translation="MITTKFTETFGVDHPVVQGGMQWVGRAELVAAVANSGALGFITA
                     LTQPTPADLANEIARCRELTDKPFGVNLTILPTINPPPYDEYRRVIVESGIKIVETAG
                     SNPAPHLPMFHENGIKVLHKCTSVRHAVKAQSLGVDGISIDGFECAGHPGEDDVPGLV
                     LIPAAAEKIEIPMIASGGFVDARGLVAALALGADGINMGSRFMCTVESAIHQNVKEAI
                     VAGSELDTELIFRPLRNTARVASNAVSREVVQILDNGGQFADVKDLVAGVRGVKVFEV
                     GDLDAGIWSVGTAMGLINDIPTVGELVSRIVDEAEELIAGRLARMVGSDEEERVPA"
     gene            complement(11511..12299)
                     /locus_tag="VF874_11670"
     CDS             complement(11511..12299)
                     /locus_tag="VF874_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX9176692.1"
                     /translation="MEIKDAVALVTGGASGLGLATVRALLDDGAQVVIVDLPSSKGEE
                     VAKELGGREEQKGSRDRVRFAPADVTDTDAVTAALDVAESLGPLRVVVNCAGTGSAIK
                     VLSKHGVFPLDDFKRVVNVNLIGTFNVLRLGAERIAKTEPLGEERGVIINTASVAAFD
                     GQIGQAAYSASKGGVVGMTLPIARDLAREGIRVVTIAPGLFDTPLLALLPDEAKKSLG
                     AQVPHPSRLGKPEEYGALAAHIVANPMLNGEVIRLDGAIRMAPR"
     gene            12378..13211
                     /locus_tag="VF874_11675"
     CDS             12378..13211
                     /locus_tag="VF874_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9176693.1"
                     /translation="MTTDAVSAPRVRIDSSTSVDGLMITLADGVLSITMNRPDSLNSL
                     TEAMLSGIADAVERAADDDRVRVVRLGGAGRGFSSGAGIGEEDQKGGGTSSPTKIIDE
                     ANRAVRALTALPKPAVAVVHGPAAGVAASLALGCDVVLASEKAFFMLAFTKIGLMPDG
                     GASALIAAAVGRVRAMRMALLAERVPAREALDWGLVTAVYPAEEFGAEVDKVVAALVD
                     GPALAFRKTKQAINAATLTELEDAFDREKQGQSVLITSHDFVEGTLAFQQRRSPTFTD
                     S"
     gene            complement(13208..13801)
                     /locus_tag="VF874_11680"
     CDS             complement(13208..13801)
                     /locus_tag="VF874_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9176694.1"
                     /translation="MARTQQQRREETVARLLDASIAAIVEVGYARASAAEITKRAGVS
                     VGALFRHFETMGDFMAATAHEVLRRQLDLGTKAVAETPADRPALDAMLAILRDVTRNP
                     TNAVMYELMIAARTDEKLRATLQDVLIEYGAKIYDTFRGLPGADTVPENTLAALVATL
                     INTFDGAAIVRAVVPAPEIDAQQITLLASLLNEAYGF"
     gene            complement(13803..15566)
                     /locus_tag="VF874_11685"
     CDS             complement(13803..15566)
                     /locus_tag="VF874_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9176695.1"
                     /translation="MTYGVIIRNGLWFDGTGAAPTTRTLGIRDGVVSTVSAGALDETG
                     CPEVIDAAGKWIVPGFIDVHTHYDAEVLLDPGLRESVRHGVTTVLLGNCSLSAIYADS
                     ADAADLFSRVEAVPRKYVLGALNAKKTWSTPAEYVAALDDLPLGPNVGSLLGHSDLRT
                     SVLGLGRATSDNVRPTDAELDKMAQLLDEALDAGLLGMSGMDAAIDKLDGDRYRSRAL
                     PSTFATWRERRKLIKVLRKRGRILQSAPDVKNPVSALLFFLSSSGTFGRRKGVRMSLL
                     VSADAKSNPAAVNVFGPGTRLLNALLDASVRFQHLPVPFELYSDGIDLPVFEEFGAGT
                     AALHLRDQLERNKLLADTDYRRRFRRQFDRIKLGPTLWHRDFHDAVIIECPDASLIGK
                     SFGQIADERGLHPLDAFLDVLVDNGERNVRWTTTVANHRPAQLDKLAVEPTVHMGFSD
                     AGAHLRNMAFYNFSLRLLKRVLHGQFMTVEHAVHRLTAEVADWFGLDAGTLREGDRAD
                     FVVIDPAGLDDSVDGYHEEKVPFYGGLSRMVNRNDAAVVATGVSGAVVYRNGEFCEGY
                     GTTVKSGRFLRAGKQPAPVGV"
BASE COUNT         2537 a         5380 c         5119 g         2668 t
ORIGIN      
        1 ctgaatccgc ttggccatcg accccgggaa gcggtgcacc gcaggacgtt ccgagcacag
       61 cgcagcgaac ttctccgggt cgtagtcggc gatgagccgg gcgtcgatgc cgccgatgcg
      121 gtcggcgatt ttcttcgggc cagcgaatgc cgtctccatc ggcacctgct gatcgagcag
      181 catgccgatc agaagagcca tcggattgtc gtcgagcagc gcgtcggccg ccggatcgaa
      241 tacgagctgc aatttcggca ccgacccagt ctagaacccc gcatttgcga cgtggctctc
      301 actagtctca cagggaccgc cggttctata tcaggagtcg cctgtgccca cactcaagca
      361 gcatttggag atccaggcag gtgcttcctt acgggtatcg gctcggaaaa ggctgtgaca
      421 cactgagttc cagtcggcgg acgaggttcg ccttcccact gctgacgtac gtgttcgcgg
      481 cgatcatgct cggcacgacg ctgccgacgc cgatgtacgc cctgtattcc gaacgcatgc
      541 acttctcggt gctgacgacc acggtggtgt tcgccacgtt cgcattcggc gtgctggccg
      601 cgctgctggt gttcggccga tggtcagacg cggtgggccg ccgcccaatg ttgttgctag
      661 ccacgatgtt tgcgctcggc agtgcggtgg cgttcttggc ggccaccggg gtcgacgacc
      721 tgctgctggg caggctgctg tccggactgt cggccggtgt ggccaccggc acggcaacgg
      781 cggccgtgat cgaggcggcg cccgaacagt ggcggggtcg cgcggcggcg atcgcgaccg
      841 tggccaacat cggcggcctc gggctcggac cgctggttgc cggctgctgg tgcagtacgc
      901 gccggaacca ctcaacctct cgttcgtcgt gcacataatg ctgatgttcc tcgccgcagc
      961 gagcctggcc gcggtacccg aaaccagccc gcgcactggc cgcctcggat tccagcggct
     1021 ctcggtaccg gcccaggtgc gcccggtgtt cgtcgtcgct gccaccgccg ccttcgccgg
     1081 tttcgcggtg ctcggcctac tcaacgcggt gtcgccgtcg ttcgtcgcgg gcatcatcgg
     1141 catcggcaac catgccgtcg ccggcgcgat tgtctgctcg atcttcgcca tcttcgccgc
     1201 gtcggcgtgc gcacagctgg cgtcgggccg gatcgaggcc aaccgggcgg tcgccgtcgg
     1261 ctgtgccgta ctggtaatcg gaatgctgat catcgctgcg tccctttatt tttcgaacct
     1321 ggcgctgctg atcctcggcg ccgtggtcgc gggcgtcggc cagggcatca gtttcagccg
     1381 gggcctggca gcggtcgcag aacagacacc gccggatcgg cgcgcggagg tcagctcgac
     1441 ctacttcgtc gtcgcctata tggcgatctc actgccggtc atcggcgagg gcctggcggc
     1501 cgaggcgtgg ggcctgcgga cggcgggaat cgtgttcgcc ctggcggtcg ccgcgttggc
     1561 ggcggtgtgc ctggccgcaa ttctcgtgca ggaggcgcac ctcaaccgcc ggtagattcg
     1621 cgtccgtggc actgtttccc agcccggccg agatcgccgc gcagacgccg cccgcccgcg
     1681 accgcgcgat cgacgtcatc cgcatcgtgt cgctggtcgc ggtggtgttc gggcacacga
     1741 tcatggcggt cagcaccgta cgcgacgacg tgttcgtgtg gggcaacctg ctgagctccg
     1801 tgccggcgtt aggggccctc acctgggtgc tgcagatcat gccgctgttc ttcttcgcgg
     1861 gcacggcagc gtgcgtggag tcgtgggctc ccggccacag ctggggcaac tggctgatgc
     1921 aacgctgcac ccgcctgttc cgcccggtgt tcttctacct cgcgttttgg ggcgtcgcgc
     1981 tggccgtgct gcggttcgtg ttgcccgagc atgtctacga gccggtggcc ggaaccagca
     2041 ttcagctgct gtggttcctg ggcgcctacg tgttggtgct cgccgcgatc ccgctgctct
     2101 accggatcac caccaccaag cggctggccc tcggcgtgct ggccgtgtac ggcttggtcg
     2161 tcgcggtaga cgccgtgcgg ctcaacgtcg acgggctctc cgtgctcgga tatctgaacg
     2221 tcgtcgtctg gctgatccca ggcatgttcg gcgtcgccta ccggcgcggc ctgctgtgcg
     2281 ggctcgccgc gaaagcggtc gccgcgctga tgctcgtggt caacgtgctc ttggtgtggc
     2341 tcggaccgta cgagttgagc ctcgtcggct accaggagca gcggctgccc aacatgacgc
     2401 cgccgtcggc gctgctggcc gggcacgcgg tcatgatgtg cgcggtggcg atcgcggccg
     2461 cgcccgcgat cgcgcggtgg gcacagcggc cacgggtgtg gtggctggtg gcgatcggca
     2521 actccggcgc gatgacgctg tatctgtggc acatgcccgc actactgggc atgcacctgg
     2581 tgttcgacgc gctcggcctc ccccgctacg acgtgaccgc ccccgacttc gtcgcgctgt
     2641 cggtggtgca gctggtgttg atggcggtga tggtcggcgt gctgttcgtc gcgctgcggc
     2701 cgttggagaa caacccgctc ccgggctggg acggcggctt cgtcgcccgg cccggtgcgc
     2761 gcagcgccgc ggtgggcagt ctgctgtgcg cgaccggcac cgcgacgctg gtgtcggtca
     2821 ggtgggggct caaggacgac gggctgatct gcgtcgccgt gcttctcgtc gcgctgatcg
     2881 cggcacgggc cctggctacc gacgcgaaag tgcccgccgc agcgcctgtt tcgggctaac
     2941 cactcttgcg gcggaactcc cgccgatgct cggccggacc gtgcgtccgg ggctgcttgg
     3001 cgccggtgtc cttgtgctgc tcgcccccgg acgctttggc gttcttgcgt tccaacgcct
     3061 cgcggaactt gcgcttggtg tcgtcctcgg gagtaccgtc cgctgccata cccacaggct
     3121 agccgcgcta gcggagtccc ggtggcattc cgtagacgtg cgagatcggc agccgcatca
     3181 gcacccgccg atcggtgacc atcgcgcggc ggtagtcgtc ccagtccggg tgctcgcccg
     3241 cgatgttgcg gtacaacgca atcagcgcct cgacggtgtc gtcgtgcggg tcggcggccg
     3301 gcgggctcag ctcggcggtc ccctccgcga ccgcgtacga ccagccgtcg tcagaggtga
     3361 cgagaatcga ggcccgcgga tcgcggcgca ggttgcgggt cttggcccgc ggctcagtga
     3421 tcgacacctc gatgacggca gtgcgcgagt cgaagtgata cgacacattc gacagctgcg
     3481 ggcgcccgtc gcgcttgatg gtggccagca cgcccagcga gttcccgctg atcacggcca
     3541 gcagcttgtc gtcgaatgct tgcggcgcca tacacgcgag cctacgtggg acgtcctacg
     3601 attgatcagc atggccagat acgcggcatt cctgcgcggc gtcaacgtcg gcggggtcaa
     3661 cctgaagatg gccgacgtgg ccgaggcgct caccgaggcc ggcttcagcg acgtccggac
     3721 catcctggcc agcggcaacg tgctgctcga atcccggtcg aaggtagcgg cggtgcgcac
     3781 gacagcggaa gccgcgctga gggaggcgtt cggatacgac gcgtgggtgc tggcctacga
     3841 cgtcaagacc gttcaagcca tcgtcgacgc ctacccgttc gaacgtgaag tgcccgacca
     3901 ccactcctac gtgacgttcg cttccgaccc cgaggtgctc gacgagctcg ccgcgctcga
     3961 gcccggtccc gacgaacgaa tcgagcgcgg caagggcgtg atttattggc aagtgccacg
     4021 gtcgaagtcg cttcagagcg ccatgggcaa gaccatgggc aagaagcgct acaagtcgtc
     4081 gaccaccacc cgcaacctgc gcacgctcga cagggtgctg cgccggtaga gttcggcctg
     4141 atgacctctt ctaacggcca ccgggccgac gtgacgacac tgaccggtgt atcggagacg
     4201 gcgctgctga cgctcaacgt acgggccagg gaagcccgac ggcccgacgc gatcatcgag
     4261 gacccgatgg ccatcaagct cgtcgactcc atcgactacg acttcgcgaa attcggcccg
     4321 tcgaaccgcc aggacgtcgc agtgcgcgcg ttggccttcg accacaacgc ccgacgctat
     4381 ctcgcggccc atccgcacgc caccgtggtc gcgctggccg aagggctgca gaccagcttc
     4441 tggcggctgg acgagcccgg cgaccagttc cattggctga cagtcgattt accacggatc
     4501 atcgaattgc gtcgcaggct gctgcctcgg tcgccgcgaa tctctgaacg tgcgcagtcg
     4561 gcgctggact tctcctggat ggacgacgtc gatccggcgc agggggtgtt cgtcaccgcc
     4621 gagggactgc tgatgtacct gcagcccgac gaggcgatgg gcctgatcag agaatgcgcc
     4681 cggcggtttg ccggcggcca gatgatgttc gacctgccgc cgcgctgggc cgcggccctg
     4741 gcccgccacg ggttccgggc gtcgttgcgc tacaaggtgc cgccgatgcc gttcagtctg
     4801 tcgctggccg aaatcgctga tctggtcaac accgtcccgg gcatccgcgc cgtgcacgac
     4861 atgccgctgc cccgcggccg cggacccgta ttcaacctgc tgttgtggac catccagcgg
     4921 ctgccgctgt tcgacccggt ccggccggcg atcaccctgc tcgaattcgg ctagggacct
     4981 gctgaattgc ggcactgcgg agcatttcct ggacgactac tgttgcccgc cttatctgtt
     5041 gttgctgatc gatctactct cgttatggcc gtgacctggg taaatgctgt tgtcgcgcag
     5101 aggcgggcaa gtccaactat cccgccgaag gccagctagc gcggaccgaa cgccgcctcc
     5161 acgaaacgca ccgcgtcggc cgacccgacc cccagcgccc tggcggtgtc gacgtagatg
     5221 cgtgcggcgg ccaccatccg cgcgtcgacg ggatcgacgc cacagacgaa tgtgcctgcg
     5281 cggccccgtg tttcgaccat tcccgccgtt tcgagctccc ggtatgtccg ggcgacggtg
     5341 ttcaccgcca acccgagctg cccggccagg gcccgcacgg tcggcaatcg cgtgccggcg
     5401 gccagccgcc cgtcgcggat gccgtcgatg atctgggttc tcagctgatc gaacagcggt
     5461 gtgcccgcct tgctgtccag ccgaacccag tcggccaact cggacacctg ctcaacatag
     5521 cgcgctgcag ctaccttgga cctgtgcgcg tgacggtgct cagcggagcc gggatgtcgg
     5581 ccgaaagcgg ggtcgcgacg ttccgtgacg cccagaccgg gctgtgggag acgttcgatc
     5641 ccgaggagct cgccagcccc gcggcctggc tgcgccagcc ggacgtggtg tgggcgtggt
     5701 acctgtggcg ccaccacctg gtgcggtccg tcgaacccaa ccccgggcat tacgcgctcg
     5761 ccgactggca ggaccgtgcc gacgtgcgcg tcgtcaccca gaacatcgac aacttgcacg
     5821 agcgggccgg cagtcaacac gtcgcgcacc ttcacggcag cctgttcgac tttcgctgtt
     5881 cgcgatgcgg cgagcggtat cacggcgagc ttcccgacat gccagagccc gtcggggcgg
     5941 ccgacccgcc gctgtgtgcg tgcggaggcc acatccgccc cggggtggtg tggttcggcg
     6001 aggcgctgcc cgacgagccg tggcagcagg ccctcgacgc ggtggcaggc gccgacgtgg
     6061 tggtggtggt gggcacgtcc ggaatcgtct accccgcagc gggattgccg tccgcggcgc
     6121 tggcgcaggg cataccggtc gtcgaggtca accccgacga cacgccgctg tcgcgcagcg
     6181 cgacggtcac tgtccgcgac accgccgcca acgcgctgcc gagactgctg gaccggctcc
     6241 acgaattgct cggctgacaa ctacgccggc cgaacggccc gccccaacgt gtgctcggac
     6301 acgggcaccg gcacccaggc aggcagcgtc cagtcgtgac gggcggtctg ctgaacgaat
     6361 cccgcgttga cgatcgcagt ttcggtgtcg cggtgggtat ggcagttgcc ggccagccgc
     6421 ggccagatgg tggcatccgc gagtcgctgc agtcgacccc gggcgccgct gctcgccacg
     6481 tgctccaggt agcgcagctc gccacccggg cgcagcatcc aaaacagttg ccgcagaacg
     6541 gcttcctggt cctccaccga gcacagcacc agcgagcaga cgacggcgtc gaacggctcg
     6601 cccggctcga tgtcctcgac ggcgcggtcc atcacagtga caggcacggg tgcggtcgcg
     6661 gcggtccgtt gcgcgatcgc gttcaaccgc ggctcgggct caaccgcgac cacctcttcg
     6721 acggaggtcg gatagtgcac gaagttggag ccggttcctg cacccacctc cagcacgcgg
     6781 cccgacagac tggcgaggtt ctcccgcctc agctcgcgca gcgactcggg ctcgtaccgc
     6841 gacagcaccg tccacatccg cgcgaagaac gggttgtcga cgcggttgct actcatggtc
     6901 tggccctctg agtttcatga tggcgttaat ggcgtcctgc gggcaggtgt cgtagccgat
     6961 tatcccgctg acgatctgac cgcagcacac cgcctgcagc accctgtcgg tgaacgtctc
     7021 cgcgtcaccc cacggcagat acggtttggc gatcagcatg gccgccaccc cgtgcgcagc
     7081 cgtccacatc tccagcgcgg tctgcgtggc gtcgccgtcg acgtagatgc cctcgtcaat
     7141 cagcgcctgc acggaagcgc gcatgtgcac gaacgccgaa ctgttcagcg tgacgtcgac
     7201 gtcgctgccg ggcctgccct cgctcatcgt cgcgatccgg tacagctccg gcgtctgcag
     7261 cgcgaaccgc acgtaggcct ggccctgcgc gcgaagcacg tccagtgtgg tggtctgccc
     7321 gaccgcggcc cgctgcattt cttcgttgag cttttcgaag tagcgcgcgc acaccgcgtc
     7381 cagcagcgcg tccttgtccg tgaagtgcag gtagatcgac ggcggcgtga cgccgacgcg
     7441 ctgggccacc gaccggatcg aaaccgcttt ggcgtgaccg gtttccagca acaaatcggt
     7501 ggcggcgtca aggatctcgt cgcgcagctg gtcgccggag ccgcgaggcg cccgccgcct
     7561 gcggtcggaa ctacgaacgg gtgccgacac gttgaccagt ctccgtcatt gccggctcgg
     7621 atccgccccg agcctcgctg atcccgaatc ggtcgtgcag ccgcgccaac ggcttcggcg
     7681 cccaccagtt cgcccggccc agcacgtgca tgaacgccgg caccagcagc atccgcacca
     7741 acgtggcgtc caccaaaacc gcgagcgtca ggcccacacc gaacatgcgc atgaacgcca
     7801 cctgagccgc gatcagcgcc gcgaacgaga tcgacatcag aagcgccgcg gcggtcacca
     7861 cgcgtccggt gcgagccaag ccgagcgcga cgctctcgtc gttgcgcacg cgaggagcaa
     7921 gagagctcct gtcggcgcgg gtcctccccg attgcagcca gtactcgcgg atccgcgaga
     7981 tcaggaacac ctcgtagtcc atcgacagac cgaacgcgat gcagaacaac agcactggca
     8041 tgttcgcgac gagcgtaccg gtggaggtgg tcccgaacgc gcctaagtgg ccgtcctgga
     8101 aaatccagac cagcgcaccg aacgccgccg agagcgataa tatgttgagc aacaaggctt
     8161 tcagcggcaa caccacgctg ccggtcagca tgaacagcag cacgaacgtg atcgccgcga
     8221 tcacggccag cacggtgggg agccgcgacg tgacggcctg gctgctgtcg cggttgactt
     8281 gcgctgtgcc ggtgagcaac acgtcgtggc cggccggtgt cgagacagcg tgcagccggt
     8341 cgagctggtc ctccgaggcc ggcgtgaaga gcggttcggt gctgccgatc gtcaggaatg
     8401 cgcggcggtc cttgacgccg gtcgccgccg acggcggtcc ggccagcgcg ccgcgcacgt
     8461 agctgccgtc cggcgccgag accgacgaga cgtccggcac cttcgacagg tcggcggcat
     8521 aacggctcag ttcctccggg gtgacgccgg acatgtccgg aatgaccgcg atcacgttgg
     8581 tggcggagtc gaccgcgaag tcgctgcgca ggctgtcgcc gatctgacgc gcggacgccg
     8641 actgcgacag cacgcgatcg tcggggaagc cccacttcgc acccaggaac ggtgcaccca
     8701 gcaccatcag cagcgcgaca atcgcgatac ccaccggaat cgcatggcgc atcacgaatt
     8761 tcgtcgaccg gtaccagaat gtctgctcga ccggcttctt cactggctca ggccgatgca
     8821 gcagccgccg accaagcttc cgcacgtcca gcgaatcaag ccggtcaccg agcaacgcga
     8881 ttgccgcagg cgtcacgatg atcgccgcgg ccgccgcgaa cgccacgacc gcaacaccgg
     8941 cgtacgcgaa cgacttcagg aagtacatcg ggaacaagac catcgcgacc atcgacagcg
     9001 cgaccgtcat cgcggaaaac aacacggtgc gaccggcggt cgccatcgtg cggatcagcg
     9061 cctcgtcgcg gccggcgccg tcggcgatct cgtcgcggta gcggctgatg atcagcagcg
     9121 tgtagtcgat cgccagcgcc agccccatcg cgacggtcag gttcagcgca aagatcgaca
     9181 cgtccgtcgc gaacgtgatc gcgtgcagca ccgccatcga cccgaggatc gcgaagccgc
     9241 ccaccgccaa aggcaacgcg gctgcgagca ggccaccgaa cacccagacc agcacgagaa
     9301 agctcagcgg gatcgcgatc gactccatca gcagtaggtc tttttcgctc tggccgttga
     9361 tctgcacgta gatcatcgcc tcgccgccgg ccttgaccgt gacgccgccg cggtcgtgca
     9421 ccacctgact ggtcagggtc ttggcacgct gctgcgcttc gctttcggtg cccgagatgc
     9481 ctgcgacgat cagaccggtc ttgccgtcct tgctcaccag cgccggcgcc acggacggtg
     9541 gcgacgtcca cgccgagttc acctgggcga tgaacggcga ctctgaaagc tgacgcgcga
     9601 tatccgtgcc ggtctgtcgc gcctctggac tctgggcgcc cggctcgcac gtgacgctga
     9661 tgaccagcgt caagtcgccc tggtcgaacg tttcggagag cattttcttg gcctcggccg
     9721 actgcgatgt cgggtcttgg aagccgcctg cggacaggct tttggtgacc gggatgccga
     9781 agaccgcggt gccgaccagc accagcagcg ccagcccgat gatgcgccgg ggcgcggcga
     9841 tagccaatcg ggcgattccg gtcagcacga tgcgtccttc cggcaggcgg cgtatcagcg
     9901 gtaacttatc agcgataagt cagcgcggtc aagatgtcga aggaccgttc caacggggat
     9961 acgactgctg ctcccgcaca gttcatccac gaggtttgac aggaagttgc gacatgttcg
    10021 aggggataaa gcgcggctgg aacatcgcca aggacagctg ggggtcttca gtgcactcgg
    10081 cagcatcttc cgaaccgcgg tctgtgtcta cgccactacc ggtcaggccc caggaagaat
    10141 ggatcccacc cttgtgcagg gcgcgtttta cccgaaatac cccgaaacga cgcgcgccgc
    10201 gaccccggaa gcctacccgc gggtaatatt gccagcgttt tccgaaacgt gactcaaagt
    10261 cctcttaaag gcgatccata cgctcagtga catgtggatc gatgacacca gcgctgacgt
    10321 catcaaggtt gacttcgacg cgctttacca cggcgatgta ctggtagagg gcgacacctc
    10381 ggagcaactc gacgactggc acccgctggc acccgctgtc tgaccgcggc ggaacaggtc
    10441 ggcgcgtgag tttgctccgc gccgaccttg gtatttgtgg ttcttaagcc ggaacccttt
    10501 cctcttcatc tgatccgacc attcgggcca gccgcccggc aatcagctcc tcggcctcgt
    10561 cgacgatccg tgacaccagc tcgccgaccg taggaatgtc gttgattagg cccatcgccg
    10621 tgccgaccga ccagatgccc gcgtcgaggt cgcccacctc gaacaccttc acgccacgca
    10681 cgcccgccac caagtccttg acgtcggcga actgaccgcc gttatcgagg atctggacca
    10741 cctccctgga caccgcattg ctggccaccc gcgccgtgtt gcgcaatggc cggaaaatca
    10801 gctcggtgtc cagttcggag ccggcgacga tcgcctcctt gacgttctgg tggatcgccg
    10861 actcgaccgt gcacatgaac cgcgagccca tgttgatgcc gtcggcgccc agcgccagcg
    10921 ccgcgaccag accgcgcgcg tcgacgaacc cgccggaggc gatcatcggg atctcgatct
    10981 tctccgcggc cgccgggatc agcaccaggc cggggacgtc gtcctcacct ggatgcccgg
    11041 cacactcgaa tccgtcgatg ctgatcccgt ccacgccgag gctttgcgcc ttgaccgcgt
    11101 gccgcaccga cgtgcacttg tgcagcacct tgatcccgtt ttcgtggaac atcggcagat
    11161 gcggcgccgg gttggacccg gcggtttcga cgatcttgat gccggattcg acgatcacgc
    11221 gccggtactc gtcgtagggc ggcgggttga tcgtcggcag gatcgtcaga ttcacgccga
    11281 acggcttgtc ggtcaactcc cgacagcggg cgatctcgtt ggccagatcc gccggagtcg
    11341 gctgcgtcag cgcggtgatg aatcccaaag cgcctgaatt cgccaccgcc gcaacgagtt
    11401 ccgcgcggcc gacccactgc atgccgccct gcacgaccgg gtgatccacc ccgaacgtct
    11461 cggtgaactt cgtcgtgatc atctgtgccc tctcctcttc gcgcaagcgc tcatcggggc
    11521 gccatccgaa tcgccccatc caggcggatc acctcgccgt tgagcatcgg gttcgcgacg
    11581 atgtgcgcgg ccagtgcgcc gtattcctcc ggcttgccga gacggctcgg atgcggcacc
    11641 tgggcaccga gcgacttctt cgcctcgtcg ggcagcaacg ccagcagcgg cgtgtcgaac
    11701 agccccggcg cgatcgtcac cacgcgaatt ccctctcgcg ccaaatcccg tgcgatcggc
    11761 aacgtcatgc ccacgacgcc gcctttcgac gccgaatacg ccgcctgccc gatctgtccg
    11821 tcgaacgccg ccaccgacgc ggtgttgatg atcacgccgc gctcctcgcc cagcggctcg
    11881 gtcttggcga tccgctcggc gcccagccgc agcacgttga acgtgccgat caggttgacg
    11941 ttgacgacgc gcttgaagtc gtccagcggg aagacgccgt gcttggagag caccttgatc
    12001 gcggagccag tgcccgcgca gttgacgacg acgcgcagcg gaccgagcga ctccgcgacg
    12061 tcgagcgcgg cagtcacggc gtccgtgtcg gtgacgtcgg cgggcgcgaa gcgcacgcgg
    12121 tcgcgggatc ctttttgctc ctcgcgtccg cccagttcct tggcgacctc ttcgccctta
    12181 ctgctcggca ggtcgacgat caccacctgc gcgccgtcgt cgagcaacgc cctcacggtg
    12241 gccaggccga gcccggacgc gccgccggtg acgagcgcta cggcgtcttt gatctccact
    12301 gatcgattcc tttcttcggt gagcacgcga gccgacccac tggttggttg aactatacaa
    12361 agccctaagg tgtagccatg acgactgacg ccgtttctgc tcctcgcgtc cgcatcgatt
    12421 ccagcacgag cgtcgacggc ctcatgatca cgcttgccga cggggtgctg tcgatcacga
    12481 tgaaccgacc ggacagcctc aactcgctca ccgaggcgat gctgtcagga atcgccgacg
    12541 cggtcgaacg cgccgccgac gacgaccgcg tgcgggtggt gcgactcggc ggcgccgggc
    12601 gcggcttcag ttcgggcgcg ggaatcggcg aagaagacca aaaaggcggc ggcacaagct
    12661 ctcccaccaa gatcatcgac gaggccaacc gcgcggtccg cgcgctcacc gcgttgccga
    12721 agccggccgt cgccgtcgtg cacggcccag cagccggggt ggcggcgtca ttggcgctcg
    12781 gctgcgacgt cgtattggcc tcggagaagg cgttttttat gctggcgttc acgaagatcg
    12841 gcctgatgcc cgatgggggc gcgtcggcgt tgatcgccgc ggcggtcggc cgggttcgcg
    12901 cgatgcgcat ggcgttgctc gcggaacggg ttcccgcccg cgaggcgctg gactggggtc
    12961 tggtcaccgc ggtatatccc gccgaggagt tcggcgcaga ggtcgacaag gtggtggcgg
    13021 cgttggtcga cggccccgca ttggcgttcc gcaagaccaa gcaggccatc aacgccgcca
    13081 cgctcaccga actcgaggac gcgttcgatc gcgagaagca aggccagtcg gtgttgatca
    13141 cgtcgcacga cttcgtcgag ggcacgctcg ctttccagca gcgccgctcc cccaccttca
    13201 cggacagcta gaaaccgtaa gcctcgttca gcaacgaggc cagcagcgtg atctgttgcg
    13261 cgtcgatttc gggtgccggg acgacggccc gcactatcgc ggcaccgtcg aaggtgttga
    13321 tcagtgttgc gacgagcgca gcgagcgtat tctccggcac ggtgtcggca ccgggcaacc
    13381 cgcggaaggt gtcgtagatc ttcgcgccgt actcgatgag cacatcttgt agtgtggccc
    13441 tgagcttttc gtcggtacgc gcggcgatca tcagctcgta catcacggca ttagtgggat
    13501 tgcgggtgac gtcgcgcagt atcgccagca tcgcgtcgag agccggccgg tctgccgggg
    13561 tctccgcgac cgctttggtg cccagatcaa gctgacggcg cagcacttcg tgtgccgtgg
    13621 cggccatgaa atcgcccatg gtctcgaagt gccggaacag tgcgcccacc gacaccccgg
    13681 cgcgcttggt gatctccgcg gccgacgcgc gcgcataccc gacttcgacg atcgcggcga
    13741 tgctggcatc gagcaggcgc gcgacggtct cctcccggcg ctgctgctga gttcgtgcca
    13801 tctcagacgc cgacgggtgc cggctgtttg cccgcgcgca ggaaccggcc ggacttcacc
    13861 gtcgtgccgt atccctcgca gaactcaccg ttgcgataga cgacggcgcc gctgaccccc
    13921 gtcgcgacca ccgccgcgtc gttgcggttg accatccggc tcaatcctcc gtagaacggc
    13981 accttttcct cgtggtagcc gtccaccgag tcgtcgagtc ccgccgggtc gatcaccacg
    14041 aagtcggcgc gatcgccctc gcgcaacgtg cccgcgtcga gcccgaacca gtcggcgacc
    14101 tcggcggtca gccgatgaac cgcatgctcg acggtcataa actgcccgtg caagacccgc
    14161 ttgagcagcc gcagcgagaa gttgtagaac gccatgttgc gcaggtgagc gccggcatcg
    14221 gagaaaccca tatgaacggt cggctcgacg gcaagcttat cgagctgcgc gggcctgtga
    14281 ttggcgaccg tggtcgtcca gcgcacgttg cgttcgccgt tgtcgacgag cacgtcgagg
    14341 aacgcgtcga gcgggtgaag cccgcgctcg tcggcgatct gcccgaaact cttaccgatc
    14401 agactcgcgt cagggcactc gatgatgacg gcatcgtgaa agtcgcggtg ccacaacgtc
    14461 ggacccaatt tgatgcggtc gaactggcgg cggaaccggc gtcggtaatc agtgtcggcc
    14521 aacagcttgt tgcgctcgag ttgatcgcgc agatgcaaag ccgcggtgcc ggcgccgaac
    14581 tcctcgaaaa ccggcagatc gatcccgtcc gaatacaact cgaacgggac cggaagatgc
    14641 tggaaccgaa cgctggcgtc cagcagcgcg ttgagcagcc gcgtgccggg cccgaacacg
    14701 ttcaccgcag cggggttcga cttggcgtcc gccgacacca gcaggctcat ccgaaccccc
    14761 ttgcggcgcc cgaatgttcc gctgctggac aggaagaaca gcagcgccga caccgggttc
    14821 ttgacgtcgg gtgcgctctg caagatccgg ccgcgcttgc gcaacacctt gatcagcttg
    14881 cgccgctcac gccacgtcgc gaacgtcgac ggcagcgccc gcgagcggta ccggtcgccg
    14941 tcgagcttgt cgatcgccgc gtccattccg gacatgccca gaaggccggc atcgagcgcc
    15001 tcgtcgagca actgcgccat cttgtccagt tcggcgtccg tcggccgtac attgtcagag
    15061 gtggctcgac ccaaacccag caccgaggtt cgcaggtccg aatgcccaag cagcgagccg
    15121 acattcggcc ccagcggcag atcgtccagc gccgcaacgt attccgcggg cgtcgaccac
    15181 gtcttcttgg cgttcagcgc cccgagcaca tacttgcgcg gcaccgcctc gacccggctg
    15241 aagaggtcag cggcgtccgc cgagtccgcg tagatcgccg acagcgagca gttgcccagc
    15301 agcacggtgg tgacgccgtg ccgcaccgac tcccgcaagc cggggtcgag aaggacctcg
    15361 gcgtcgtagt gggtgtgcac gtcgatgaag ccggggacga tccacttgcc cgccgcatcg
    15421 atcacctccg ggcagcccgt ctcgtccagc gccccggcgg acacggtcga cacgacgccg
    15481 tcgcgaatgc ccaaggtgcg ggtcgtgggc gctgcgccgg tcccgtcgaa ccagaggccg
    15541 ttgcggatga tcacgccgta agtcatgcca tcaagctaac atagatagtg agtactcgca
    15601 accttttatt attgccctgc cgagagaccg aaaatcggcg gcaacgccag gaccatgagc
    15661 gcgctccacc agaaatgcgt caggatcggg gcgagcacgc cgcc
//
LOCUS       DASWBF010000322         3748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_550343,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000322 DASWBF010000000
VERSION     DASWBF010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550343"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..697
                     /locus_tag="VF874_11690"
     CDS             71..697
                     /locus_tag="VF874_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin-binding hemagglutinin"
                     /protein_id="HEX9176696.1"
                     /translation="MTDGGNGGDIGTNVVEDLKAPLFAAVGAADLALERVNEIIAALR
                     DRVEDARTDATDRVEETRGRLTKLQDELPERAQDIRGRLSGDELRRAAEGYREQATEL
                     YNDLIARGEAVLERLRSQPGFEERRAQAEKRFSEAAELTQEALGNVASQTRAVGERAA
                     KLIGRDVPKKAAKKAPAKKTTAKKATAKKAIAKKAPAKKGAAKRVTQK"
     gene            740..1015
                     /locus_tag="VF874_11695"
     CDS             740..1015
                     /locus_tag="VF874_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HEX9176697.1"
                     /translation="MNDLVRTVLIVLGFAVFVATVYAFVHAAMQRSDAYTATGKLTKP
                     VWLVILGVSALLSIVPSIMGPIIGACAAGVYLVDVRPKLLEIQGKSR"
     gene            1019..1423
                     /locus_tag="VF874_11700"
     CDS             1019..1423
                     /locus_tag="VF874_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2599 domain-containing protein"
                     /protein_id="HEX9176698.1"
                     /translation="MRVALLAAALAALPLAVGVAAPAHAGGPPPPPYIDRTEWVHWAD
                     LSSLRVYPTLAGREASTEFTSPDEAWSEVLVAAPDADLPGMREQFDCHWQLAELAEPG
                     KTSWNLEPWRPEVTPAQMLKAGCNPGNVEEPF"
     gene            1440..2111
                     /gene="deoC"
                     /locus_tag="VF874_11705"
     CDS             1440..2111
                     /gene="deoC"
                     /locus_tag="VF874_11705"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX9176699.1"
                     /translation="MPTRAELAKLVDHTLLKPEATPADVEAAVAEAVELGTYAVCVSP
                     SMVSAAISARPPDGPVIATVAGFPSGKHRAEVKAREAALAVDDGAAEIDMVIDLGAVL
                     AGDLDAVRDDIAVVRQAIPDATLKVIVESAALLELRGPATLVDVCRVAEDAGADFVKT
                     STGFHPSGGASIRSVELMADAVGDRVGIKASGGIRTAADALAMIRAGATRLGLSGTRG
                     VLDGL"
     gene            complement(2106..3071)
                     /locus_tag="VF874_11710"
     CDS             complement(2106..3071)
                     /locus_tag="VF874_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HEX9176700.1"
                     /translation="MTNPPGPPYGDPRSWGPPGNQGHPGPPRGPLPPREAPTQHVPRP
                     GSPIDGPTQHIPKAGPPGAPAGPPPTPPAAKKRRFSLRDPLSIVLILVIVLAVVAAGL
                     LGAELYARHKANSKITAAAECVSEDNVSVSVSSMPPFLWQHINGHYDNITVTTAGNQI
                     QSAKGMKAEVSINDVDLQGDGNSKGTIGALDAKITWTAEGISQTIQEAIPVVGSFVGD
                     VKTNASDGTIQLDGTFGDIVAKPQVVDGKIKLQVESLSGLGFTLPSESVQPALDEMTN
                     KFADELPLGIKADSIQVTDDGVVGQFSTRNAQIPQGESDPCFADL"
     gene            complement(3068..>3748)
                     /locus_tag="VF874_11715"
     CDS             complement(3068..>3748)
                     /locus_tag="VF874_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9176701.1"
                     /translation="GVPLAPVAEPLHGPWASAVRSIAADAGVVVVAGMFVPSGDGRVT
                     NTLIATGPGVEAHYDKIHLYDAFGFTESHTVAPGHEPVVIDVAGVGVGLTTCYDVRFP
                     ALYTELADRGAKVMTVSASWGSGPGKLEQWTLLVRARALDATSFVAAAGQAYPGPEVA
                     TKGAPMGVGGSLIASPLGEVVADAGADPAVVVADIDLHAIDDARNTIAVLRNRSSFAQ
                     PDRAESRG"
BASE COUNT          585 a         1234 c         1315 g          614 t
ORIGIN      
        1 ttgctggtat ggcccttgaa gtgtgtttgg ccattgctgt tcgtgctgta gtccttgggg
       61 atggggaatc atgactgacg gtggaaacgg cggagacata ggcaccaacg ttgtcgagga
      121 cctgaaggct ccgttgttcg ccgcggtcgg cgccgccgat ctggcactgg agcgggtcaa
      181 cgaaatcatc gccgcgctgc gtgatcgcgt cgaggatgcc cgcacggacg ccaccgaccg
      241 ggtcgaggag acccgtgggc gcctcaccaa gctgcaggac gagcttcctg agcgcgctca
      301 ggacattcgt ggccggctgt ccggcgacga gctgcgcagg gccgccgagg gctaccgcga
      361 gcaggccacc gagttgtaca acgacctgat cgcgcgcggc gaggctgtcc tggagcggct
      421 gcgcagccag cccgggttcg aggagcgtcg ggcgcaggcc gagaagcggt tcagtgaggc
      481 cgccgagctg acccaggaag cgctgggcaa tgtcgccagc cagactcgcg cggtcggcga
      541 gcgcgccgcc aagctgatcg gccgcgatgt gccgaagaag gccgccaaga aggccccggc
      601 gaagaagacc accgccaaga aggccaccgc caagaaggcc atcgccaaga aggcgccggc
      661 caagaagggc gcggccaaga gggtcaccca gaagtagcgc agtcgacgat cgggacgatg
      721 cgcgcctagg cttgttcaca tgaacgacct ggtgcgtacc gtcctgatcg ttttggggtt
      781 cgccgtcttc gtcgcgacgg tctacgcgtt cgtccacgct gcgatgcagc gcagcgacgc
      841 ctacaccgcc acaggcaaac tgaccaagcc ggtgtggctc gtcatcctcg gcgtgtctgc
      901 gctgctttcg atcgtgccct cgatcatggg gccgatcatc ggggcctgcg ccgcaggcgt
      961 ttatcttgtc gacgtccgac cgaaacttct tgaaattcaa gggaagtcgc gctagcggat
     1021 gcgcgtggcc ctgctcgccg cggcactcgc cgcgctgccc ttggccgtgg gcgtcgccgc
     1081 gcccgcgcac gcgggcggcc cgcccccgcc cccgtacatc gaccgcaccg aatgggtgca
     1141 ttgggcggac ctgtccagcc tgcgggtata cccgacgctc gccggccggg aggccagtac
     1201 ggagttcacc agcccggacg aggcatggtc tgaggtgctc gtcgccgcgc ccgacgccga
     1261 ccttcccggc atgcgtgagc agttcgattg ccattggcag ctggccgagc tcgccgaacc
     1321 gggcaagacg agctggaacc tcgagccgtg gcgtccggag gtcacccccg cccagatgct
     1381 caaggcaggc tgcaatccgg gcaacgtcga agaaccgttc taatgccaac gggcgtctaa
     1441 tgccaacgcg cgccgagctc gcgaagctcg ttgaccacac cctgctcaaa cccgaagcga
     1501 caccagccga cgtcgaggcc gcggtggccg aggcggtcga actcggcacc tacgcggtgt
     1561 gcgtgtcgcc gtcgatggtg tcggcggcga tctccgccag accgcccgac gggccggtaa
     1621 tcgcgacggt ggcgggcttt ccgagcggca agcaccgcgc ggaggtcaag gcgcgggagg
     1681 cagcgctggc ggtcgacgac ggcgccgccg agatcgacat ggtgatcgat ctcggagcgg
     1741 tgttggcggg tgaccttgac gcggtgcgcg acgacattgc cgttgtgcgc caagcgattc
     1801 cggatgcgac gctgaaggtg atcgtcgagt cggcggcgct gctcgagttg cgcgggccgg
     1861 ccacactggt cgacgtgtgt cgcgttgccg aggacgccgg cgccgatttc gtcaagacct
     1921 ccacgggctt tcaccccagc ggtggtgcgt cgatccggtc cgtcgagctg atggcggacg
     1981 cggtcggcga tcgggtgggc atcaaggcca gcggcggcat ccgtaccgcc gcggacgcac
     2041 tcgcaatgat ccgagcaggc gccacccggc tgggcctgtc gggcacccgc ggggtgctcg
     2101 acggcctata ggtccgcgaa gcagggatcc gattcgccct gcgggatttg cgcgtttcgc
     2161 gtcgagaatt ggcccaccac accgtcatcc gtgacttgga tcgagtcggc cttgatgccc
     2221 agcggcagtt cgtcggcgaa cttgttggtc atttcgtcca gcgcaggctg taccgactcg
     2281 ctcggcaggg tgaagcccag gccggacaag ctctccacct gcaacttgat cttgccgtcg
     2341 acgacctgcg gcttggcgac gatgtcgccg aacgtgccgt ccaactggat ggtcccgtcg
     2401 gatgcgttgg tcttgacgtc gccgacgaag ctgccgacca ccgggatcgc ctcctgaatg
     2461 gtctggctga tgccttcggc agtccaggtg atcttcgcgt cgagcgcgcc gatggtgcct
     2521 ttggagttgc cgtcaccctg caggtcgaca tcgttgatcg agacttcggc cttcatgccc
     2581 ttcgcgcttt ggatctggtt tcccgcggtg gtgacggtga tgttgtcgta gtggccattg
     2641 atgtgctgcc acaaaaacgg cggcattgaa ctgaccgaca cgctcacgtt gtcctcggac
     2701 acacattcgg cagccgcggt gatcttgctg ttggctttgt ggcgggcata cagctcggca
     2761 ccgagcaagc cggcggcgac gaccgccagg acgatgacga ggatgagcac gatcgacagc
     2821 ggatcgcgca ggctgaaccg gcgcttcttc gccgctggcg gggtcggcgg tggccccgct
     2881 ggcgcgcccg gcgggccggc cttcgggatg tgctgtgtgg gcccgtctat cgggctgccc
     2941 ggccgcggaa cgtgctgggt cggcgcctcc cgcggcggaa ggggtccacg cggggggcct
     3001 gggtgcccct ggttgcctgg gggaccccag gatctcgggt cgccgtacgg cgggccaggg
     3061 ggattcgtca cccgcgtgat tctgccctat ccggctgggc aaaggatgag cgattgcgaa
     3121 gtacggcgat cgtgtttcgg gcgtcgtcga tggcatgcag gtcgatgtcg gcgacgacca
     3181 ctgccggatc agcgccggcg tcggcgacca cctcgcccag cggtgaggcg atcagactgc
     3241 cgccgacgcc catcggcgcg cccttggtcg ccacttccgg gcccgggtac gcctgcccgg
     3301 cggctgcgac gaagctggtg gcgtccagtg cccgggctcg caccagcagc gtccactgct
     3361 ccaacttgcc cggacctgac ccccacgacg cgctgaccgt catcaccttg gcgccccggt
     3421 cggccagctc tgtgtacagg gccgggaagc ggacgtcata gcaggtggtc aaccccacgc
     3481 cgacgccggc cacgtcgatc acgacgggct cgtgaccggg cgcaaccgta tgcgactcgg
     3541 tgaacccgaa cgcgtcgtag aggtggatct tgtcgtagtg ggcctcgaca cctggcccgg
     3601 tggcgatgag cgtgttggtg acccgcccgt cgccgctggg cacgaacatg cccgcgacga
     3661 cgacgacgcc ggcgtcggcg gcgatcgatc ggaccgccga cgcccacggt ccgtgcagcg
     3721 gctcggccac cggcgccagc gggacacc
//
LOCUS       DASWBF010000323        24874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_879103,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000323 DASWBF010000000
VERSION     DASWBF010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24874
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879103"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VF874_11720"
     CDS             complement(<1..755)
                     /locus_tag="VF874_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX9176702.1"
                     /translation="MQRLVAWLNRAGYLRKWLILGITIGIIAGLGAVVFYVALEYAGR
                     FLLGYLADYHVPQPKGDGGDPGSAGYTRPWAIPLVAFGGALVSALLVAKFAPEAEGHG
                     TDSAIEAVHTDPRAIRGRAIVVKTIASALTIGSGGSAGREGPAAQISAGFGSLLTRQL
                     NLSDGDGRIAVSLGIGSGIGAIFGAPLGGAVLAASIVYREDFDYRALIPGFITSATGY
                     AVFGSILGWDPMFGDVASDFQFDQPAQLIWFVAL"
     gene            1110..1739
                     /locus_tag="VF874_11725"
     CDS             1110..1739
                     /locus_tag="VF874_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9176703.1"
                     /translation="MTALPDWDIVSDAELACAAAAGDRRAFASIYDRYADRLHDFCIG
                     MLRDREAAADCVQDVFCHAATRLSQLREADKLRPWLYAIARNEALRCIRSRNRERTYD
                     EVPETESGEAGPDTLAARSELANLIAEAAGGLSDRDRSVLELAYRHGLDGPELAEALG
                     VSAANAKKMTQRLRETIERSLGALLVSRRVRSNPAACPELGEILDGWDG"
     gene            1752..2576
                     /locus_tag="VF874_11730"
     CDS             1752..2576
                     /locus_tag="VF874_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176704.1"
                     /translation="MRKRIARHIESCAVCDEERSRLVNPVALLGGTPVFIPAPAWLRD
                     RTLDQINLTASSTPIGSTEATQTAPAAAEDEPTALLETGDAADAESKFLSRRTLLPAF
                     GVLAILVALGMLTVAWLQRDSSVAPANVTKSPPASQMPRVVAPSPSSPPPPVAPSEGP
                     VTTTIPAITSVPPPVESTVAPPLVTTTVPVAPPPPPSRPGPSSGTSTAPVFTPPSTAD
                     EPPPPPETETEAPTATQPPVTTKPPTKPPTKPPVTKPPTATVGPASPPTTTAGPIL"
     gene            2632..4764
                     /locus_tag="VF874_11735"
     CDS             2632..4764
                     /locus_tag="VF874_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9176705.1"
                     /translation="MTEHDVHDAPAANHTVHCGGGDGGHGDHVAQYRRLFWLMLVLAI
                     PTVAASGMFAMILGYTIPDIPGIRWVSPVLGTVIFVWGGRPFLTGAVSEIRSRAPGMM
                     LLIGLAITVAFVSSMGSSLGLLGHELDFWWELSLLIVIMLLGHWIEMRSLAQTSSALD
                     SLAALLPDEAERVEGDEQVRVPPSQLRVGDLVVVRPGGSVPADGEIVEGTAAMNESMV
                     TGESRPVSRGPGERVVAGTVATDSGLRIRVTAVGDDTALAGIQRLVTEAQNSSSRAQR
                     LADTAAGWLFWFALGSAIITAIVWGFFGHPDQAVIRTITVLVIACPHALGLAIPLVVS
                     IATERAARGGVLVKDRLALESMRTVNAVLFDKTGTLTKGEPAVTGIAAARGSDDDVLA
                     LAAAAESDSEHPLARAIVEAARRRELSVPRWTDFSSSPAVGVTATVDGRIVRVGGPRM
                     LEEAGLTELAETAAWHDEGAIVLHVVADGAVVGALKLADEVRAESRQAVDALHRLGIR
                     VVMITGDAPAVAEAVAGDLGIDRVFAGVRPEDKAAKVSELQSEGLKVAMVGDGVNDAP
                     ALAQADVGIAIGAGTDVAIASAGVILASSDPRSVLSVIQLSKATYRKMKQNLWWAAGY
                     NLISVPLAAGVLAPVGFVMPMSVGALLMSLSTVVVAVNAQLLRRLDLTPEASVRAVLG
                     DSAVNAHRDREHGRESDEITTMTSVSAK"
     gene            complement(4774..5031)
                     /locus_tag="VF874_11740"
     CDS             complement(4774..5031)
                     /locus_tag="VF874_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX9176706.1"
                     /translation="MHTKTVIVGSAIGLHARPAAIIAEATVKAGVPVTLSVDGGEPVD
                     AGSALMIMTLGAGNGAQVTVASEDEQVMQTIADLVQQDLDA"
     gene            5058..6758
                     /locus_tag="VF874_11745"
     CDS             5058..6758
                     /locus_tag="VF874_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX9176707.1"
                     /translation="MTASSAPTSQKATVLRGVPAVAGVAYAPAIRPGRSPVMDDVDSL
                     PDVEKSVRPAEAARFTAAASVVADRLRERAQHTTGAASEVLATTAGLAQDRAWLGAAE
                     NRIEAGTPAVRATVQAVEQFVDLFTQIGGLMAERVTDLRDIRDRVVAELFGLPEPGVP
                     QPAQPSVLCAEDLAPADTAGLDPALIVGLATTFGGPTSHTAIIARQLGIPCVVAVAGL
                     DDVAAGAMVLVDGTLGTVTVSPGEAAATAAVDKARRDAELLASWSGPGATADEHAVSV
                     LANVQDGAAARAARQTPAEGVGLFRTELCFLNRDREPSVAEQTAIYTEVLQAFSGLKV
                     VIRTLDAGSDKPLKFVRHPEEANPALGVRGFRISEGNPGLLERQLEAIAAAGADTGSS
                     PWVMAPMIATADEAKTFAALARSYGLTPGVMIEVPAAALLADKVLEHVDFLSIGTNDL
                     AQYAMAADRMSAELAALTDPWQPAVLALVAATARAGAAAGKPVGVCGEAAADPLLACV
                     LAGLGITSLSAAAAAVPGVGAKLASVTLQQCRDAADAVVGTASPAAARAAAREILGAE
                     "
     gene            6770..7741
                     /locus_tag="VF874_11750"
     CDS             6770..7741
                     /locus_tag="VF874_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEX9176708.1"
                     /translation="MCTRVLWSDNDLAVLAGRSMDWPESTEPLVVAFPRGRERDGGAV
                     AGQTVVAQNPLRWTSRYRSLATTVYGIGTVDGLNERGFAAHGLYLQETDFGVRDPSRP
                     GLHAGLWVQYLLDRAASVGEALELLDGIQIVMVTARGHDATIHLALEDVSGDSAVIEF
                     DHGRQVVHHGREFTLMTNDPPYNQQLELLSRHDFSHPSRDMPLPGNVNAVDRFQRAAY
                     YSALMPKPETERQAVASVMAVMRNVSVPFGAPYGEFGVYNTEYRTVTDLTNLRYFFEL
                     TTSPNVIWVDFGTLDLPDGADPQAIDPYDETLIGNVTSRFAPRPLPF"
     gene            7787..8794
                     /locus_tag="VF874_11755"
     CDS             7787..8794
                     /locus_tag="VF874_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9176709.1"
                     /translation="MPAITADTLTLPRIAVAGPADTERAVRSVTTGPHGYEGEGFPVV
                     RAFAGVPAAALDPFVHMDQMGEVEYQPGEPRGTSWHPHRGFETVTYMIDGRFAHQDSH
                     GGGGLITDGATQWMTAGSGILHIETPPAELVESGGVFHGIQLWVNLPRAAKFASPKYQ
                     AIEGNQVALLSSADGGALVRIIAGAIGDYSDSMSPALRLRGPGSTYTPITLAHATIEP
                     GARLNVPWNRDFNALVYVLSGRGTVGPVGHPIQQGQLAVLGPGDRITVTAAHDQDGNR
                     PALEVLLLGGKPIREPVFAYGPFVMNSKSDIIRALEDYESGRFGRIPPDALMPHRAGG
                     S"
     gene            complement(8766..10916)
                     /locus_tag="VF874_11760"
     CDS             complement(8766..10916)
                     /locus_tag="VF874_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX9176710.1"
                     /translation="MPVPVVLHRLKARDPERDGLRRAVRAAIVVPVAAAVSFRIGASQ
                     TPLFTIFGSFALMVLADFPGNRQNRALAYAGLGVNGAVLITLGTLVAPHPWLAVTTMF
                     VLGVVVTFSGVLSETIAAGQRATLLTFVLPACTPPGPINERLLGWLIALAICVPASLF
                     LLPPRHHDELRRHAAQVCGALADRLAGNASGQQVTAAMNALRANFLGADFRPVGLTAG
                     SRALVRVVDDLEWITERIGAGTGEVLDDMKEPAVRVLRDSARVLSISRPADRAPSRDE
                     LAAALDELRAVARGRYREDIAAVLGEPDDGSAVAVGRRLLTRRTVAATIGATGRVIAA
                     AAAADARPVWARALGRQLPETGASDRVLPETAAVASITAGFLATRSVALRNSLRTGLG
                     LALAVAITHIFPVQHGFWVVLGAMSVLRSSALTTGTNLVRAVVGTTIGFVIGALLITV
                     LGVDPVVLWVLLPVLVFASAYVPEVASFTAAQAAFTMMVLVIFNLIVPTGWRVGLVRV
                     EDVVVGAFAAMVVSVLLWPRGAKVVVNATIESARETFSRYLQAAVHRITRGASEAVDD
                     NLTDLGHDGLVAWRTLDDAVRQYLSESGGATDSRAPVVRASNRVMRMRTAADLIADIQ
                     WPPPLTAYPRAKKILEAHTDAVCDRLAGRDDTVWPPISDDVVPALRAESTGGDSAVAA
                     ALPLIMVAASLGELELIYPSAAEALVTTRRRGAA"
     gene            complement(10943..11515)
                     /locus_tag="VF874_11765"
     CDS             complement(10943..11515)
                     /locus_tag="VF874_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9176711.1"
                     /translation="MATTSLSREAYFDTGLDMLSDLGYGGLKLAEVCNRLGVTSGSFY
                     HNFSSWPAYTHELVNHWMMRASTTKLMDCCGREPDPRRRIDQLIRNGLRLPHDTEAAI
                     RTCSNLDPEVHAAQETVDRARHDIMREAAHQVLQDERQAQVFADWAVYLLIGYEQCSL
                     PKDKTSLKWIAGQLLECLDDGRFASIARRA"
     gene            11647..12279
                     /locus_tag="VF874_11770"
     CDS             11647..12279
                     /locus_tag="VF874_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9176712.1"
                     /translation="MPKASRGPGRPPAAKAADTRERIVRAARQVFSELGYDAATFQEI
                     AVRADLTRPAINHYFKSKKLLYREVVERTNEMVVLKGVETAFAATTLLGQLEGFISAA
                     VGSDLEDRSAAAFLVTSVLESHRHPELRREDHDALLGTRQFLTWAVTGAIERGELTTE
                     TPVAQVVEVMTAMFWGMGFYAGFIGSHEGLEMITEHFKKLMIGKLWDWKA"
     gene            12348..13286
                     /locus_tag="VF874_11775"
     CDS             12348..13286
                     /locus_tag="VF874_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9176713.1"
                     /translation="MSQPQISSVRTPGDSWEITESVGATALGVAAARAAETAQSDPLI
                     RDPFAYLLVCTAGPGWAELAGGSAPWYGDDERGRTIDEMSRSYQAVRTHFFDEYFGAT
                     CRSGIRQVVILAAGLDSRAYRLNWPAGTAVFEIDQPKVLAYKSATLEAHGAVPNATRH
                     TVAVDLRDNWPAALIDAGFDPRRPTAWLAEGLLPYLPADTQDRLFEMVTALSAPGSRI
                     AVEAFTPHTSQSDEQRAAWRERTARLRQQLGLDDDFEALIYQEPDRADAARWLEEHGW
                     QVHAVDSHDEMARLGRPVPEELVEESVSSTLVSARL"
     gene            13327..14274
                     /locus_tag="VF874_11780"
     CDS             13327..14274
                     /locus_tag="VF874_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9176714.1"
                     /translation="MSALRTDDDTWDIATSVGSTAVLVAAARAAETARPDRLISDPYA
                     EILVAGAGTGIWEQMLDDELPAKLAAVDPGAAAIFEYMRSYQGVRTHFFDAFFADATA
                     HGVRQIVILASGLDSRAYRLDWPAGTVVYEIDQPKVLEYKAATLAAHGAQPSAERHEV
                     PIDLRQDWPAALRAAGFDPSQPTAWLAEGLLMYLPGAAQDRLFEQITALSAAGSRIAV
                     ETAGRHAPERREVMRERFERIADQIGMQEGPDITDLTYDDPDRADVADWLNAHGWAAT
                     GEPSHEVQRRLGRHIEVPMNDGDAFATFVTAERAPQPVG"
     gene            14307..15722
                     /locus_tag="VF874_11785"
     CDS             14307..15722
                     /locus_tag="VF874_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9176715.1"
                     /translation="MTTESVAPAPQAAAAPASEVAETVARLRRTFATGRTRSVEWRQD
                     QLRKLEKLMVDNEPAIAAALDADLGRKPFEAWLADVSSVAGEAKDAAKNVRKWIRRKY
                     RLLELSQLPGRGWVEYEPYGTVLIIGAWNFPFALTLGPAVGAIAAGNTVVLKPSEVAP
                     ASSALMAELVARYLDNEAIAVVEGDGAVSQKLIAQGFDHLLFTGGTEIGRKVYEGAAP
                     HLTPVTLELGGKSPVIVSADADIDVAAKRIAWTKLLNSGQICIAPDYVLADAKICDEL
                     VDKIKDAVTTFEAGETGGKPIVNKRHFDRLTNSLAATKGTVAVGGGSDAAQCRIEPTV
                     VVDPDPDEPLMTDEIFGPILPVLTVQSLDEAITFVNSRPKPLAAYLFTKAKAVRERVI
                     REVSAGGMLVNHLVFHFATNKLPFGGVGPSGMGAYHGKFGFEEFSHRKSVMTKPTRPD
                     LTAMIYPPYTEKAWKLARRIF"
     gene            15763..16626
                     /locus_tag="VF874_11790"
     CDS             15763..16626
                     /locus_tag="VF874_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9176716.1"
                     /translation="MPGVQDRVVVVTGAGGGLGREYARTLAREGASVVVNDLGGARDG
                     TGAGSAMADQVVAEIREAGGRAVANYDSVADADGAAGIVKTALDEFGAIHGVISNAGI
                     LRDGTFHKMPFENWDAVLKVHLYGGYNVIRAAWPHFREQSFGRIVVATSTSGLFGNFG
                     QANYSAAKLGLVGLINTLAQEGAKYNIKANAVAPIAATRMTEDILPPEVSKKLTPEYV
                     APVVAYLCGEEVPDTASVFIVGGGKVQRAALFQNEGVTFSDVPSVDDVAGRWNEIDDL
                     SAAKQASFSVG"
     gene            16633..17600
                     /locus_tag="VF874_11795"
                     /pseudo
     CDS             16633..17600
                     /locus_tag="VF874_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
     gene            17943..18425
                     /locus_tag="VF874_11800"
     CDS             17943..18425
                     /locus_tag="VF874_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEX9176717.1"
                     /translation="MSATGTFLVALAIFVGLVGIVVPLLPGLLLVYAAILVWAVVEHT
                     VASWVTLGVVTVIVGATTTVKYLWPVRRLRGADVPTSTLLVGAVLGIVGFFVIPVLGL
                     ALGFVGGVYLAELARRRDQRRAWASTVHAVKGVALSIGVELTGGLVAAAIWLLAVLLS
                     "
     gene            complement(18529..19323)
                     /locus_tag="VF874_11805"
     CDS             complement(18529..19323)
                     /locus_tag="VF874_11805"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX9176718.1"
                     /translation="MPDLKGAWNFRDVADATGVRPGRFFRSSELSALEDDGRDELRAL
                     GVFDVADLRSPREVERRGPGRVHDDVAIHPLPFPDLAEDADGEAPHEHSFTRMLTEQP
                     DDEPPAEAAVRYMTDEYRRFPTLGGARNAVRQVISLLASGRPVITHCFAGKDRTGFTV
                     AVVLESLGVDRDAILDDYLRSNTAVPQLRERILTMVHERAGSELTPEAVMFAEARLSD
                     EVLGVRDEYLTAARQSIDDNYGSLDAYLQESGVSPADIDALRTALL"
     gene            complement(19326..20537)
                     /locus_tag="VF874_11810"
     CDS             complement(19326..20537)
                     /locus_tag="VF874_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176719.1"
                     /translation="MSAKAQDYHKRLSAFMTEFVFPAEAEYERYRLERGPDDHSVPPV
                     IEELKKLARERGLWNLFLPSVSGLTNTDYAPLAELTGWSMEIAPEATNCAAPDTGNME
                     TLHLFATEQQRKQWLEPLLAGEIRSAFSMTEPAVASSDARNIETSIVRDGSDYVINGR
                     KWWTSGASDPRCKILIVMGRTNPDAASHQQQSMVLVPIDTPGVTVLRSTPVFGWQDQH
                     GHCEVIYDKVRVPTENLLGEEGSGFAIAQARLGPGRIHHCLRAMGAAERALALMVDRA
                     RNRWAFGKPLAEQGVVRESIAKCRNEIDQARLLCEKAAWTIDQHGNKAAHLLVSQIKA
                     VAPKVACDVIDRAIQVHGAAGVSDDTPLAQLYSWHRAMRIFDGPDEVHMRTIARAEIG
                     REKSAFVAAVT"
     gene            20653..21840
                     /locus_tag="VF874_11815"
     CDS             20653..21840
                     /locus_tag="VF874_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9176720.1"
                     /translation="MSSTDLWRARRRHHTRSLRIAAVLRLAVVIVMVAAMLLGTAHAD
                     WPKQSVLLVVYGLMAVCVAVLAFSRVGVSVIGLRQRFVFAFADVGFLFAFQLLSTGGY
                     IPLVLMALVPLIVVPQLSWRQAVVVLSVSVAAFVYAVLQDDVIPVQLGWRDSVFLFVM
                     YGFLCCTGLLVAYVEEHHVDEIAALTASREELLGQIMTASETERRRISEAIHDGPLQD
                     VLVARQEIGELAASSRSEELDRAMVCLRDASQRLRDATFELHPAVLEQVGLGAAVEQL
                     ATFTATRARIAVDTNIDYPAQQPIDPIMFGVVRELLSNVVRHSQASRAWITLVVTDSM
                     CRLDVADNGIGMTDERAARRLAEGHIGLASHRARVEAAGGKFTIADEEAGTHVRVELP
                     LPD"
     gene            complement(21841..22494)
                     /locus_tag="VF874_11820"
     CDS             complement(21841..22494)
                     /locus_tag="VF874_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9176721.1"
                     /translation="MPGPAANEKVRVVVADDHPLVREGVARALTASGSVDVVGEAEDG
                     TTALEMIRTQRPDVALLDFRMPGLDGAQVVTAVRRDELPTRVLILSAHDESAIVFQAL
                     QEGAAGFLPKEANKTEIVNAVLACAKGRDVVSPSVAAGLAGEIRRRAEPTGPVLTPRE
                     REVLAGISEGKSIPAIAAQLYLAPSTVKTHVQRLYEKLGVGDRGAAVAEAMRRKLLE"
     gene            complement(22943..24082)
                     /locus_tag="VF874_11825"
     CDS             complement(22943..24082)
                     /locus_tag="VF874_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176722.1"
                     /translation="MPVDRLLPTDEAADLIVLARDLADKVLTPIVDAHEKAESYPEGV
                     FPQLGAAGLLSLPQPQEWGGGGQPFEVYLQVLEEIAARWAAIAVAVSVHSLSCHPLLT
                     FGSDDQKQRWLRGMLSGEQIGAYCLSEPQAGSDAAALRCAAKRSDGGYVINGSKSWVT
                     HGGRADFYMLFARAGEGSRGISCFLIPASLPGLSFGKPEEKMGLHAVPTTSAFYDHAH
                     LDADRLIGGEGQGLQIAFSALDSGRLGIAAVAVGLAQAALDEAAAYANERTTFGRKII
                     DHQGLGFVLADMAAAVTSARATYLDAARRRDAGRPYSTQASVAKLIATDAAMKVTTDA
                     VQVFGGVGYTRDYRVERYMREAKIMQIFEGTNQIQRLVIARALTK"
     gene            24125..>24874
                     /locus_tag="VF874_11830"
     CDS             24125..>24874
                     /locus_tag="VF874_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX9176723.1"
                     /translation="MTDSQTSPDVAGRAARGPDVAGQAAPTIGVVRESGDGERRVALV
                     PKAVHALAGRGLAVVVESGAGDGALLPDDLYTEAGATIGDAWGADVVVKVAPPTADEV
                     GRLRSGQSLIGFLAPRNADNQVDALTKAGVQAFALEAIPRISRAQTMDALSSQANVGG
                     YKAVLVAASEATRFFPMLTTAAGTVKPATVLVLGVGVAGLQALATARRLGARTSGYDV
                     RPEVAEQVRSVGAQWLDLGIDAAGEGGYAREL"
BASE COUNT         3935 a         8345 c         8647 g         3947 t
ORIGIN      
        1 agtgcgacga accagatcag ttgtgccggt tggtcaaact ggaaatccga cgccacgtca
       61 ccgaacatcg ggtcccagcc gaggatcgaa ccgaacacgg cgtagccggt cgccgacgtg
      121 atgaagcccg ggatcagcgc ccggtagtcg aagtcttcgc ggtacacgat cgacgcggcc
      181 agcaccgcgc cgcccagcgg tgcgccgaag atcgcgccga tccccgagcc gatgccgagc
      241 gacaccgcga tgcggccgtc cccgtcggag aggttgagct ggcgggtcag cagcgagccg
      301 aagccggcgg agatctgtgc ggccggtccc tcgcggccgg cggatccgcc cgagccgatg
      361 gtcagcgcgc tggcgatggt cttcacgacg atcgcgcggc cccggatcgc ccgcgggtcg
      421 gtgtgcaccg cctcgatcgc gctgtcggtg ccgtgccctt cggcttcggg cgcgaacttg
      481 gccaccagca gcgctgacac cagcgcgccg ccgaatgcga ccagcgggat cgcccacggc
      541 ctggtgtagc cggccgaacc cgggtcgccg ccgtcgccct tgggttgcgg gacgtggtag
      601 tcggcgagat agccgagcag gaatcggccg gcgtattcca gggcgacgta gaagacgacg
      661 gccccgagcc cggcgatgat gccgatcgtg atgcccagga tcagccattt gcgcaggtag
      721 cccgcgcggt tgagccaggc tacgaggcgc tgcatgcagc ggatgctagc gggcggatgc
      781 gcgccgaggc ggtaactgcc gcggagaagt tcgagtgacc cccacgcgga ataccgtttc
      841 ggcgcaaaaa aagtgtcccc gccgcagcgg gtgcgcgctc ctacctgtgt ccgtccgcca
      901 cccagcgacc gaaggattac ttcatgacca ccacccacta cgcgaccccc cgccagtccg
      961 cggatcccat ggcggtcgcc aaagcgctcg cactcgtcgt gctcgcattg gctgtcccgc
     1021 agccgtagaa cgtcacaact ttgtcagatg ccttcagtgc atgcctggtc cgcactacga
     1081 tcacgagtta tccacagggg aacgattcca tgaccgcact tcccgactgg gacatcgtca
     1141 gcgacgctga gttggcgtgt gccgccgccg ccggtgaccg ccgtgcattc gcctccatct
     1201 acgaccggta cgccgaccgg ctgcacgact tctgcatcgg catgctgcgc gaccgcgaag
     1261 ccgcggcaga ctgcgtccaa gacgtgttct gccacgccgc gacccggctt tcccaattga
     1321 gggaggctga caagctccgg ccgtggctgt atgcgatcgc ccgcaacgag gcgttgcggt
     1381 gcatccggtc gcggaaccgc gaacgcacct acgacgaggt gccggagacg gagtcgggcg
     1441 aagcgggtcc cgacacgctg gccgcgcgca gcgagctggc gaacctgatc gccgaagcag
     1501 ccggcggtct gtccgaccgc gaccgctcgg tcctcgagtt ggcgtaccgc catggactgg
     1561 acggccccga actcgccgag gcgctcggag taagcgccgc caacgcgaag aagatgacgc
     1621 agcggctgcg cgagacgatc gaacggtcgc tgggcgcgct gctggtgtcg cggcgggtgc
     1681 gcagcaaccc ggccgcgtgt cccgagctag gcgaaatcct cgacggctgg gacgggtaat
     1741 tctccgtgct gatgcggaaa aggattgcgc gccatatcga gtcgtgcgcc gtgtgtgacg
     1801 aggaacgcag ccgtttggtc aatccggtcg cgctgctcgg cggtacgccg gtgttcattc
     1861 ccgctcccgc atggctgcgc gaccgcacgc tggaccagat caacctcacc gccagcagca
     1921 cgccgatcgg ctccacggag gcgacccaga cggcgcccgc ggcggcggag gacgagccga
     1981 ctgcgctcct ggagaccggc gacgcagccg acgcggagag caagttcctg tcgcgacgga
     2041 ctctgctacc cgcattcggc gtgctcgcga tcctcgtcgc gctgggtatg ctgacggtcg
     2101 cgtggctgca gcgcgactcg agtgtcgcgc cggccaacgt caccaagtcg ccgccggcca
     2161 gccagatgcc gcgcgtggtc gcaccgtcgc cgagcagccc accacccccg gttgcaccgt
     2221 ccgagggacc ggtcacgacg acgatccccg cgatcacgtc ggtgccgccg ccggtggaat
     2281 cgacggttgc gccgccactg gtcacgacga cggttccggt ggcgccaccg ccaccgccca
     2341 gccggccggg gccgtcgtcg ggaacctcta cggcgccggt gttcacgccg cccagcacag
     2401 ctgatgaacc gccaccgccg ccggaaaccg aaaccgaagc cccgaccgca acacagccgc
     2461 cggtgacgac gaagccgcca acaaagccgc cgacgaaacc gcctgtcacg aaaccgccga
     2521 cggcgaccgt gggacctgcc tcaccgccca cgacgactgc tggtccgatc ctctagaccc
     2581 cgggactcag gccactcgtc ctgatacccc aggggggtat atagtggcgg catgaccgag
     2641 cacgacgtgc acgacgcccc tgcagcgaac cacaccgtgc actgcggcgg cggcgatggc
     2701 ggccacggcg accacgtcgc gcagtaccgc cggctgttct ggctgatgct ggtgctggcg
     2761 atcccgaccg tagccgcgtc gggcatgttc gcgatgatcc tcggctacac gatccccgac
     2821 attcccggca tccgatgggt gtcgccggtg ctcggcaccg tgatcttcgt ctggggcggt
     2881 cgaccgttcc tcaccggtgc ggtgagcgaa attcgttcgc gcgcaccggg aatgatgctt
     2941 ctgatcgggt tggcgatcac ggtggcgttc gtgtcgtcga tgggcagcag cctggggttg
     3001 ctgggccacg aactcgactt ctggtgggaa ctgtcgctgc tgatcgtgat catgctgctc
     3061 ggccattgga ttgagatgcg gtcgctggca cagaccagtt ccgcgctgga ctcgctggcc
     3121 gcgctgctgc ccgacgaggc cgagcgcgtc gaaggcgacg agcaggtgcg ggtgccgccg
     3181 tcgcagctgc gggtcggcga cctggtggtg gttcgaccgg gcggcagcgt cccagccgac
     3241 ggcgagatcg tcgagggcac cgccgcgatg aacgagtcga tggtcacggg cgagtcgcgg
     3301 ccggtgtcgc gcggacccgg cgagcgcgtg gtggccggca ccgtcgccac cgactccggc
     3361 cttcgcatca gggtcaccgc cgtgggagac gacaccgcgc tggccggcat ccagcgcctg
     3421 gtcaccgagg cgcagaactc gtcgtcgcgg gcacagcggc tggccgacac cgccgcgggc
     3481 tggctcttct ggttcgcgct ggggtcggcg atcatcaccg cgatcgtgtg ggggttcttc
     3541 ggtcatcccg accaggcggt gattcgcacc atcacggtgc tcgtgatcgc gtgcccgcac
     3601 gcgctgggcc tggccatccc gctggtggtg tcgatagcca ccgagcgcgc ggcgcgcggc
     3661 ggcgtgctgg tgaaagaccg cctcgcgctg gagagcatgc gcacggtcaa cgcggtgttg
     3721 tttgacaaga ccggcacgct gaccaagggt gagccggccg tgacggggat cgcagccgcc
     3781 cgcggtagtg acgacgatgt gctggcactg gcggccgctg cggaatccga cagcgagcat
     3841 ccgctggccc gcgcgatcgt cgaagcggcg cggcggcggg agctttcggt gccgcgctgg
     3901 accgacttct cgtcgtcgcc ggccgtcggt gtcaccgcga ccgtcgacgg acggatcgtt
     3961 cgggtgggtg gcccccgcat gctcgaggag gcggggctga ccgagctggc tgagacggcg
     4021 gcctggcacg acgagggcgc gatcgtgttg cacgtcgtcg ccgacggcgc cgtggtcggc
     4081 gcgctgaagc tcgccgacga ggtgcgcgcc gagtcccggc aggccgtcga cgcgctgcac
     4141 cgcctcggga tccgggtcgt gatgatcacc ggcgatgcgc ctgcggtcgc cgaagcagtg
     4201 gccggcgacc tcggcatcga ccgcgtgttc gccggggtgc ggccggagga caaggctgcg
     4261 aaagtcagcg aattgcaaag cgaggggctg aaagtcgcga tggtcggcga cggcgtcaac
     4321 gacgccccgg cgctggcgca ggccgacgtc gggattgcga tcggcgcggg caccgatgtg
     4381 gcgatcgcgt cggcaggcgt gatcctcgcc agctcagatc cccgctcggt gctgtcggtg
     4441 atccagctgt cgaaggccac ctaccgcaag atgaagcaaa acctttggtg ggcggcggga
     4501 tacaacctga tctcggtgcc gctggcggcg ggtgtactgg cgccggtcgg cttcgtgatg
     4561 ccgatgtcag tcggcgcgct gctgatgtcg ctgtcgacgg tggtggtcgc cgtcaatgcg
     4621 cagctgctgc ggcggcttga cctgacgccc gaggcgagcg tgcgtgcggt gctgggcgat
     4681 tcggccgtca acgcgcaccg agacagagag catggtcgtg aatcggacga aatcacgacc
     4741 atgacttctg tttcggcgaa ataggtccgg gcctcaggcg tcgaggtcct gctggaccag
     4801 gtccgcgatc gtctgcatca cctgctcgtc ctcggacgcg acggtcacct gagcgccgtt
     4861 gccggcaccg agcgtcatga tcatcagcgc cgagccggcg tccaccggct cgccgccgtc
     4921 gaccgacagt gtgactggga caccggcctt gacagtggcc tcggcgatga tcgcggcggg
     4981 ccgggcatgc aggccgatgg cggagccgac gatgacggtc ttggtgtgca tgagcgattc
     5041 tccttactgt ttcaagaatg accgcctcgt cggcgcccac gtcacagaag gccaccgtcc
     5101 tgcgcggcgt acccgccgtc gcgggcgttg catacgcgcc cgcgatccgc cccggccggt
     5161 cgccggtgat ggacgacgtc gactcgctgc ccgacgtcga gaagtcggtg cgccccgccg
     5221 aggcagccag attcaccgcg gccgcctccg tggtggccga ccggcttcgg gagcgcgcac
     5281 agcacaccac cggcgcggcg tcggaggtgc tggccacgac ggccggcctg gcccaggacc
     5341 gtgcgtggct cggcgcggcc gagaatcgga tcgaggccgg caccccggcg gttcgggcca
     5401 cggttcaggc cgtcgagcag ttcgtcgacc tgttcaccca gatcggcggc ctgatggccg
     5461 agcgggtgac cgatctgcgc gacatccgcg accgcgtcgt ggccgagctg ttcggacttc
     5521 ccgaacccgg ggtgccgcag ccggcgcagc cgtcggtcct gtgcgctgaa gacctggcgc
     5581 cggccgacac cgcggggctg gacccggcac tgatcgtcgg gttggccacc acgttcggcg
     5641 gcccgacaag ccacaccgcg atcatcgcca ggcagctggg catcccgtgc gtggtcgcgg
     5701 tcgccgggct cgacgacgtc gcggccggtg cgatggttct ggtcgacggc acgctgggca
     5761 ccgtgaccgt gtcgccgggc gaggcggcgg ccaccgcggc ggtcgacaag gcccgccgcg
     5821 acgcggagct tctagccagc tggtccggcc cgggtgcgac ggccgacgag catgcggtct
     5881 cggtgctcgc caacgtccag gacggcgccg cggcgcgcgc ggcgcggcag acaccggcgg
     5941 agggcgtcgg gttgttccgc accgagttgt gcttcctcaa ccgcgaccgc gaaccgtcgg
     6001 tcgccgagca gaccgccatt tacaccgaag tgctgcaagc gttttcggga ctgaaggtgg
     6061 tgatccgcac gctggacgcc gggtcggaca agccgctgaa gttcgtccgc caccccgagg
     6121 aggccaaccc ggcgctcggc gtgcgcgggt tccggatcag cgaaggcaat cccgggctgc
     6181 tggagcgtca gctcgaggcg atcgccgccg ccggcgcgga caccggctcc tcgccctggg
     6241 tgatggcgcc gatgatcgcc acggccgacg aggccaagac cttcgccgcg ctcgcccggt
     6301 cgtacggcct gacgccgggc gtgatgatcg aggtgcccgc cgccgcgctg ctcgccgaca
     6361 aggtgctcga gcacgtcgac ttcctgtcga tcggcaccaa cgatctggcc cagtatgcga
     6421 tggccgccga ccggatgtcg gccgagctgg ccgcgctgac ggacccgtgg cagcccgcgg
     6481 tgctcgcgct ggtcgccgcg acggcccgcg ccggtgctgc cgccggcaag ccggtagggg
     6541 tgtgcggcga ggcggcggcc gatccgttgc tggcctgcgt gctggccggc ctgggcatca
     6601 cgtcgctatc ggcagcggcc gccgcggtgc ccggggtggg ggcgaagctg gcctcggtga
     6661 cgctgcagca gtgccgggac gccgccgatg ccgtcgtggg aacagctagc ccggcggcgg
     6721 cgcgtgcggc tgcccgggag atcctcggcg ccgagtagct tgagacccca tgtgtacccg
     6781 ggtgctgtgg agcgacaacg atctcgccgt actcgcgggc cgcagcatgg actggccgga
     6841 gtccaccgag ccgcttgtcg tggcgtttcc gcgtggtcgt gagcgcgacg gcggcgcggt
     6901 cgccggtcag accgtcgtcg cgcagaaccc gctgcgctgg accagccgat accgcagcct
     6961 cgccacgacg gtctacggca tcggcaccgt cgacggactg aacgagcggg gatttgccgc
     7021 ccacggactg tatctgcagg aaacagattt cggagtgcgg gacccgtctc gaccgggtct
     7081 gcacgcaggc ctgtgggtgc agtacctcct cgaccgggcc gccagtgtcg gtgaggcact
     7141 ggagctgttg gacggcatcc agatcgtcat ggtcaccgct cgcggacacg acgcgacgat
     7201 ccacctcgcg ctggaggacg tctcgggcga ctcagcggtc atcgagttcg accacggcag
     7261 gcaggtggtc catcacggtc gcgaattcac gctgatgacc aacgaccccc cttacaacca
     7321 acagctggaa cttctttccc gacacgactt ttcacatccc agccgcgaca tgccgctgcc
     7381 ggggaacgtc aacgccgtcg accggttcca gcgcgccgcc tactacagcg cgctgatgcc
     7441 gaagcctgag accgagcggc aggcggtggc ttccgtgatg gcggtgatgc gcaacgtctc
     7501 ggtaccgttc ggtgcgccgt acggcgaatt cggcgtctac aacaccgagt accggacggt
     7561 cacggacttg accaatctgc ggtacttctt cgagctcacc acgagcccga acgtcatctg
     7621 ggtcgacttc ggcacgctcg atcttcccga cggcgccgac ccgcaggcga tcgatcccta
     7681 cgacgagacg ctgatcggca acgtcacgtc gcggttcgcg ccccgcccgc tcccattctg
     7741 aactgctcgc gggaattaat cggaccgtgg tccggttatc gcgggcatgc cagcaattac
     7801 cgcagacaca ttgacattgc cgcgtatcgc cgtggccggg cccgccgaca ccgaacgtgc
     7861 ggtgcgctcc gtgaccaccg gcccgcacgg ctacgagggc gagggatttc ccgtcgtccg
     7921 cgcgttcgcc ggcgtcccgg cggcggcgct cgacccgttc gtgcacatgg accagatggg
     7981 tgaagtcgaa tatcagccgg gtgaacccag gggcacgtcg tggcacccgc accgcggctt
     8041 cgagaccgtc acctacatga tcgacggtcg gttcgcgcat caggactccc acggcggcgg
     8101 cggcctgatc accgacggcg cgacgcagtg gatgaccgcc ggatcgggca tcctgcacat
     8161 cgagacgccg cccgccgagc tggtcgagag cggcggggtg ttccacggca ttcagctgtg
     8221 ggtcaacctg ccacgagccg cgaagttcgc gtcgccgaag tatcaggcca tcgagggcaa
     8281 ccaggtcgcg ctgctgtcgt cagcagacgg cggagcgctc gtgcgaatca tcgccggcgc
     8341 catcggagac tactcggatt cgatgtcgcc ggccctgcgg ctccgtggcc ccgggtcgac
     8401 gtacacgcca atcacgttgg cgcacgcgac cattgagccc ggcgcccggc tgaatgtgcc
     8461 gtggaaccga gacttcaacg cgctggtgta cgtgttgtcc gggcgcggca cagtcggacc
     8521 ggtcggtcat cccatccagc agggtcagct ggccgtgctt ggtcctgggg atcgaatcac
     8581 cgtgaccgcg gctcatgacc aggacggcaa tcggcccgct ttggaggttc tgctgttggg
     8641 cggcaagccg attcgtgagc cggtgtttgc ctacgggccg ttcgtgatga actccaagag
     8701 cgacatcatc cgagcgctcg aggactacga gtcggggcgg ttcggcagga ttccaccgga
     8761 cgcgcttatg ccgcaccgcg ccggcgggtc gtgacgagcg cctcggccgc cgaaggatag
     8821 atcaactcga gctcaccgag gctcgcggcg accatgatca gcggaagcgc cgccgcgacc
     8881 gccgaatcac cgcccgtgga ttccgcccgc agcgccggca ccacgtcgtc gctgatcggc
     8941 ggccacaccg tgtcgtcgcg accggcgagg cgatcgcaca ccgcgtcggt gtgggcctcg
     9001 aggatcttct tcgcccgcgg gtacgccgtc agcgggggag gccactggat gtcggcgatc
     9061 agatcggcag cggtccgcat ccgcattacc cggttcgacg cgcggaccac aggcgcgcgc
     9121 gagtccgtcg cacctccgct ctctgaaaga tattggcgga cagcgtcatc cagcgtccgc
     9181 caggccacca gcccgtcgtg gcccaagtct gtgagattgt cgtcgaccgc ttccgacgct
     9241 ccgcgcgtga tgcggtgcac cgcggcctgc aggtagcgcg aaaacgtctc gcgcgccgac
     9301 tcgatcgtgg cgttcacgac gaccttcgcg ccccgcggcc acagcagcac cgacaccacc
     9361 atcgcggcga acgcaccgac cacgacgtcc tcgacgcgca ccagtccgac gcgccagccc
     9421 gtcggcacga tgaggttgaa gatcaccagc accatcatcg tgaacgcggc ctgtgctgcg
     9481 gtgaacgacg ccacctcggg gacgtaggcc gacgcgaaca ccaggaccgg cagcagcacc
     9541 cacagcacca cggggtccac gccgagcacc gtgatcagca gcgcgccgat cacgaacccg
     9601 atcgtcgtgc cgaccaccgc gcgcacgagg ttcgtgccgg tggtcaacgc gctgctgcgc
     9661 agcaccgaca tcgcgccgag caccacccag aagccgtgct gcaccgggaa gatgtgggtg
     9721 atcgccacgg ccaacgccag acccaacccg gtgcgcaggc tgttgcgcag cgcgaccgag
     9781 cgggtcgcca ggaacccggc ggtaatgctg gcgacggcag cggtttcggg cagcacccgg
     9841 tcagacgcgc cggtctcggg taattggcgg ccaagagcgc gagcccagac cggacgggcg
     9901 tcggcggccg cggccgcggc gatgacgcgc cccgtcgcgc cgattgtcgc cgcgacggtg
     9961 cggcgggtca gtagcctgcg gccgaccgcg actgcggacc cgtcgtccgg ctcgccgagc
    10021 accgctgcga tgtcttcgcg gtagcggccg cgggccaccg cgcgcagctc gtccaatgcc
    10081 gcggcgagct cgtcacggct gggtgcccgg tcggccgggc gggagatgct cagcacccgt
    10141 gcggaatcgc gcagcacgcg aaccgcgggc tccttcatat cgtcgagcac ctcaccggta
    10201 ccggcgccga tgcgctcggt gatccactcc aggtcgtcga ccacccgcac cagcgcgcgg
    10261 ctgcctgccg tcaggccgac cggacgaaag tcggcgccga ggaagttcgc ccgcagggcg
    10321 ttcatcgccg ccgtcacctg ctgccccgac gcgttgccgg ccaggcgatc ggccagtgcg
    10381 ccgcacacct gcgcggcgtg tcggcgcagc tcgtcgtggt gccgcggcgg cagcaggaac
    10441 agcgatgccg gcacgcagat cgccagcgcg atcagccagc cgagcagccg ctcattgatc
    10501 gggccgggcg gtgtgcaggc cggcagcacg aatgtcagca gcgtcgcccg ctggccggcg
    10561 gcgatcgtct cgctcagcac tccggagaac gtcacgacca cgccgaggac gaacatcgtc
    10621 gtcacggcca gccatggatg cggtgccacc agcgtgccca gcgtgatcag gacagcgccg
    10681 ttgacgccca gcccggcata cgcgagcgcg cggttctgcc ggttgccggg gaaatcggcg
    10741 agcaccatca gcgcgaacga gccaaagatc gtgaacagcg gtgtctgcga cgcgccgatc
    10801 cggaaactga cggcggccgc gaccggcacg acgatcgctg cccgcaccgc gcgccgcagg
    10861 ccgtcgcgct cggggtcacg cgccttcagc cggtgaagga cgaccggcac cggcatggct
    10921 ggatgctaga cgagggaagg actcaggcgc gccgggcgat gctggcgaac cggccgtcgt
    10981 cgaggcattc gagcagctgg cccgcgatcc acttcaggga ggtcttgtcc ttgggcaggc
    11041 tgcactgctc gtagccgatc agaaggtaga ccgcccagtc ggcgaacacc tgggcttggc
    11101 gctcgtcctg caggacctgg tgcgcggcct cgcgcatgat gtcgtggcgc gcccggtcga
    11161 ccgtctcctg cgcggcgtgc acttcgggat cgaggttgct acaggtgcgg atggccgcct
    11221 cggtgtcgtg cgggagcctc agcccgttgc ggatcaactg gtcgatccgc cgtcgggggt
    11281 ccggttcgcg cccgcagcaa tccatcagct tcgtggtgct ggccctcatc atccagtggt
    11341 tcacgagctc gtgagtgtag gccggccagc tactgaagtt gtggtagaag gacccactgg
    11401 tgacgccgag acggttacag acctcggcca gcttcaatcc gccgtagccg aggtcggaga
    11461 gcatgtcgag gcccgtgtcg aagtaggcct cgcgcgacag actggtcgtc gccatgtgag
    11521 ggaccataac caaaaaattg cccaggctaa tcttgtcggc ggtccctaga ggtcacagtt
    11581 tgacccctgc gtaaattaat agaccaaccc gggtgaattt attagaacgt gtggcagaat
    11641 gcgtgagtgc caaaggcgtc caggggaccg ggaaggcctc cggcggcgaa ggctgcggac
    11701 acgcgagagc gcatcgtgcg ggctgcgcgc caggtcttca gcgaactggg ctacgacgcg
    11761 gcgacgtttc aggaaatcgc ggtccgcgcc gacctgacgc ggcccgcaat caaccactat
    11821 ttcaagagca agaagctgct ctatcgggaa gtggtcgagc gaaccaacga aatggtcgtt
    11881 ctgaaaggcg tggaaacggc gttcgccgcg acgactttgc tcggacagct ggaaggcttt
    11941 atcagcgccg cggtgggctc cgacctcgag gaccgctcag cggcggcgtt ccttgtcacg
    12001 tcggtgctgg aatctcaccg ccatccggaa ctcaggcgtg aggatcacga cgcactgctg
    12061 ggcacgcgtc agttcctgac gtgggcggtc accggcgcca tcgaacgcgg tgaactcacc
    12121 accgagaccc cggtcgcgca ggtcgtcgag gtgatgactg cgatgttctg ggggatgggc
    12181 ttctacgccg gtttcatcgg cagtcacgaa gggctggaga tgatcaccga acacttcaag
    12241 aagctgatga tcggtaagct ctgggactgg aaagcttagc gcgctgcgtg atatgcggca
    12301 caccaccgca taggttgcct aacttattgg gtagtatggc tcacgaaatg agtcagccgc
    12361 agatttcatc ggtacgcacg cccggcgaca gctgggagat caccgaaagc gtcggcgcta
    12421 ccgcacttgg ggtcgcggca gcgcgggccg ctgaaaccgc gcaatcggac ccgctgatcc
    12481 gagacccatt cgcctacctg ctggtctgta ccgcaggccc gggttgggcc gaattggccg
    12541 gtggctctgc gccctggtac ggcgacgacg aacgcggccg gaccatcgat gagatgtctc
    12601 gcagctatca ggctgtgcgg acccacttct tcgacgagta cttcggcgcc acgtgccggt
    12661 caggcattcg tcaggtcgtg atcctggcgg cagggctgga ctcgcgggca taccggctga
    12721 actggccggc gggcaccgcg gtattcgaga tcgaccagcc caaggtgctc gcgtacaaga
    12781 gcgccacgct cgaggcgcac ggagcggtcc cgaacgcgac gcgccatacc gttgccgtgg
    12841 acctgcgtga caactggccg gccgcgctga tcgacgctgg cttcgatccg cgccggccga
    12901 cggcctggct ggccgagggg ctgctgccgt acctgccggc cgacacgcag gaccggctgt
    12961 tcgagatggt taccgcgctc agtgcgccgg gcagcaggat cgccgtcgag gcgttcacac
    13021 cgcacacatc gcaatccgac gagcagcgcg ccgcctggcg ggaacgcacc gcgcgactgc
    13081 gccagcagct gggattggac gacgacttcg aggcgctgat ctaccaggaa cccgaccgcg
    13141 ccgacgccgc ccggtggttg gaggagcacg gctggcaggt gcatgccgtc gacagccacg
    13201 acgagatggc ccggctgggt cgtcccgtcc ccgaggagct ggtcgaggag agcgtgtcca
    13261 gcacgctggt ttcggcccga ctctgacgtt aatcataagc ctcacaacac aattcaggga
    13321 gaagcgatga gcgcgctgcg caccgatgac gacacgtggg acattgccac cagcgtgggc
    13381 agcaccgcgg tgttggtcgc cgccgcccgg gctgccgaaa ccgcccgccc ggaccggctg
    13441 atcagcgacc cctacgcgga gatcctcgtc gcgggggccg gcaccggcat ctgggagcag
    13501 atgctcgacg acgagcttcc cgccaagctg gccgctgtcg accctggggc ggccgcgatc
    13561 ttcgagtaca tgcgcagtta ccagggcgtg cgcacgcact tcttcgacgc gttcttcgcc
    13621 gacgcgaccg cgcacggcgt ccgccagatc gtcatcctgg cgtcgggcct ggattcgcgc
    13681 gcgtaccggc tcgactggcc cgcgggcacc gtcgtctacg agatcgatca gcccaaggtg
    13741 ctcgagtaca aggcggcgac gctggccgcc cacggcgccc agccgtccgc ggagcgacac
    13801 gaggtcccga tcgacttgcg ccaggactgg ccggccgcgc tgcgagccgc cgggttcgat
    13861 ccgtcgcagc cgaccgcgtg gctggctgag ggtctgctga tgtatctgcc cggcgccgcc
    13921 caggaccggc tgttcgagca gatcaccgca ctgtccgccg ccggtagccg gattgcggtc
    13981 gagaccgcgg gccggcacgc gccggaacgt cgcgaggtga tgcgggaacg cttcgagcgg
    14041 atcgccgatc agatcggaat gcaggaaggc cccgacatca ccgacctgac ctacgacgat
    14101 cccgaccgcg ccgatgtcgc ggactggctc aacgcccacg gctgggctgc gaccggcgag
    14161 ccgtcgcacg aggttcagcg ccggcttggc cgccacatcg aggttccgat gaacgacggc
    14221 gacgcgttcg ccacgttcgt caccgccgaa agggcgcccc aaccggttgg ttaggatcgc
    14281 ggtcatccgg tcaggaagga cccgagatga ccaccgaatc cgtcgcacca gcgccacagg
    14341 cagccgccgc cccagcatcg gaggtcgcgg agaccgtcgc ccggcttcgg cggaccttcg
    14401 ccaccggccg cacccgcagc gtggaatggc gacaggacca gctgcgcaaa ctcgagaagc
    14461 tgatggtcga caacgagccg gccatcgccg cggcgctcga cgccgacctc ggccgcaagc
    14521 cgttcgaggc ctggctggcc gacgtcagca gcgtcgccgg cgaagcgaag gacgccgcca
    14581 agaacgtccg taagtggatt cggcgcaagt accggctgct ggagctctcg cagctgcccg
    14641 gacgcgggtg ggtggagtac gagccgtacg gcacggtgtt gatcatcggc gcgtggaact
    14701 tcccgttcgc gttgacgctc ggcccggcag tgggtgcgat cgccgcggga aacaccgtgg
    14761 tgctcaagcc atccgaggtc gctccggcgt cgtcggcgtt gatggctgag ctggtggcgc
    14821 ggtacctgga caacgaggcg atcgcggtcg tggagggcga cggcgcggtg agccagaaac
    14881 tcatcgcgca ggggttcgac cacttgctgt tcaccggcgg caccgagatc ggacgcaagg
    14941 tgtacgaagg cgcagcgccg catctgacgc cggtcacgct tgaactgggc ggcaaaagcc
    15001 cggtgatcgt gtccgccgac gccgacatcg acgtcgcggc caagcgcatc gcgtggacca
    15061 agctgctcaa ctccggccag atctgcatcg cccccgatta cgttctcgcc gatgcgaaga
    15121 tctgtgacga actggtcgac aagatcaagg acgccgtcac gacattcgaa gcgggcgaga
    15181 cgggcgggaa gccgatcgtg aacaagcggc acttcgaccg cctgacgaac tccctggccg
    15241 ccaccaaggg caccgtcgca gtcggtggcg gctcggatgc cgctcagtgc cggatcgaac
    15301 cgaccgtcgt cgtcgacccc gatcccgacg agccgctgat gacggacgag atcttcggcc
    15361 cgatcctgcc cgtgctgacc gtccaatccc tcgacgaggc aatcacattc gtcaactcgc
    15421 ggcccaagcc gctggccgcc tacctgttca ccaaggccaa ggctgtgcgc gaacgcgtga
    15481 tcagggaggt gtcggcgggc ggcatgctcg tcaaccacct cgtcttccac ttcgccacca
    15541 acaagctgcc gttcggcggc gtcggaccgt cgggcatggg cgcttaccac ggcaagttcg
    15601 ggttcgagga attcagccac cgcaagtccg tgatgaccaa gccgacccgg cccgacctca
    15661 cggccatgat ctacccgccg tacaccgaaa aggcgtggaa gctggcgcgc aggatcttct
    15721 agcgcccgcc tcgcgtcgcc cgtacccacg aaaggaaatt cagtgccagg agtgcaggac
    15781 cgcgtcgtcg tcgtcaccgg agcgggaggc ggtctcggcc gcgaatacgc gcggacgctg
    15841 gcccgtgagg gcgccagcgt cgtcgtcaac gatctgggcg gcgcccgcga cggcaccggc
    15901 gccggttcgg cgatggccga tcaggtggtg gccgaaattc gcgaggcggg tggccgggcg
    15961 gtggccaact acgacagcgt cgccgacgcc gacggagccg ccggcatcgt caagaccgca
    16021 ctcgacgagt tcggcgcgat ccacggcgtg atcagcaacg ccggaatcct gcgggacggc
    16081 acgtttcaca agatgccgtt cgagaactgg gacgccgtgc tgaaggtgca cctgtacggc
    16141 ggctacaacg tgatccgtgc ggcctggccg catttccgcg agcagagctt cggccgcatt
    16201 gtcgtcgcga cgtccaccag cgggctgttc ggcaacttcg gccaggccaa ctacagcgcc
    16261 gcgaagctgg ggctggtggg gctgatcaac acgctcgcgc aggaaggcgc gaagtacaac
    16321 atcaaggcca acgcggtcgc gccgatcgcg gcgacccgga tgaccgagga catcctgccg
    16381 ccggaggtgt ccaagaagct cacgccggaa tacgttgcgc cggtggtggc ctacctgtgc
    16441 ggcgaagagg tgcccgacac cgcgtcggtg ttcatcgtcg gcgggggcaa ggtgcagcgc
    16501 gcggcgctgt tccagaacga aggtgtgacg ttctccgacg tgccgtcggt cgacgacgtg
    16561 gcgggcaggt ggaatgagat cgacgacctg tccgcagcga agcaggccag cttctcggtt
    16621 ggctagcctt ccgtgaaagc ggttgtcgcc gaggagcttt ccgggccatc gggactggtt
    16681 tatcgggacg tcgacgacgt ggcggaccag atggacggtg cgatcgttat cgacgtgcat
    16741 gcggccggcg tgacgtttcc cgacctgttg gtgattcgcg gcgagtacca gatgaaggcg
    16801 gccccggggt tcatcccggc gcggaggtcg ccggtgtggt ggtgtccgct ccggaggagt
    16861 cgggcatcga gccgggcgac cgggtcgccg cggtgacgat gaacggcggc tacgccgagc
    16921 ggatcgcggt tccgccggaa agcgtgttcc cgacggcccc ggggctcgac gatgcggagg
    16981 cggcggcgct gttgcgcaac taccacacga tgtacttcgc gctggcgcgg cgtggtgcgc
    17041 tgcggccggg ggagacggta ctggtgctcg ggtcggcggg cggtgtcggc acagccgcgg
    17101 tacagatcgc aaaggcgctg ggcgccaacg ttattgccgc ggtgcaccgc gccgacgctg
    17161 tcgggtatgt ttcgtccctg ggcgccgacc tggtgctgcc gttgacggac gactggctgc
    17221 aggccgtgcg cgagcacggc ggcggcgagg gcgtgaacct ggtcgtcgac ccggtgggtg
    17281 gcgagtcgtt cgacgacgcg atacgcgcgc tggctgccga gggccggctg ctggtgatcg
    17341 gctttgcggc aggccgcatc ccgacggtca aggtgaaccg gctgctgatg cgcaacgtcg
    17401 gcgtgctcgg ggtggggtgg ggcgaatact tcctgaccca tcccggtgct gagcagttgt
    17461 tcggtcatgg cgtcggcgag ctggtcaagg cagggctcag acctcctccg ccggttcgct
    17521 atccgctgtc gaaagcccgt gaggcgctgc agctactggc tgacggcggc gtgttcggca
    17581 agctggtgct cgagccgtag ccgggcggtg ctggcgccgg cggagtactt tttagaccag
    17641 cacacggcgc agtggcccgg ctgggaggac acggatatga ccaggacgcc cctgaaactt
    17701 cccgcgatcg cgaccgtggc cgcagttccg ctcatcctcc tcagcacagg atctggaccc
    17761 gcctacgcgg acgatgtggc gttgccgact cccgctccct ctggtgttga cgtgggtcct
    17821 gaggttgaga ttggtccagg ggtcgagatc ggttcccctg ccgaatcgtc tcttgtcgac
    17881 gaccttctgg gggtgatcgg cggcggctcg gatctcggtc cgctatcgga ttgaatggtc
    17941 acatgagcgc taccggaacg tttctggttg cgctcgcgat cttcgtcggg ctggtcggga
    18001 ttgtggtgcc gctgctgccc gggctgctgc tggtgtacgc ggcgatcctg gtgtgggcag
    18061 tcgtcgagca caccgtcgcg tcatgggtca cgctgggagt cgtcacggtc atcgtgggcg
    18121 ccaccaccac ggtgaagtac ctgtggccgg tgcgccggtt gcgcggcgcc gacgtgccga
    18181 cgtcaacatt gctcgtcggc gccgtcttgg gcatcgtcgg gttcttcgtg atccccgtgc
    18241 tcggccttgc gctgggtttc gtcggcggcg tctacctcgc cgagttggcc cgccgacgcg
    18301 atcaacggcg ggcgtgggcg tccactgtgc acgcggtcaa gggcgtcgcg ctgtcgatcg
    18361 gcgtcgagct gaccggtggg cttgtcgccg ccgcaatctg gctgctcgcg gtgctgctca
    18421 gctgacgggt gagcagcagg ttctgccctc accacctcca acgtgagccc actgcgaaaa
    18481 aatcggcgaa aaagtcgcct tcagcgcaca ttcggcgcgg acagggcgct acagcagtgc
    18541 ggtgcgcagc gcgtcgatgt cggcgggcga cacgcccgac tcctgcaggt aggcgtccag
    18601 cgacccgtag ttgtcgtcga tgctctggcg agccgccgtc aagtattcgt cacgcacgcc
    18661 gagcacctcg tcggacagcc gcgcctcggc gaacatcacg gcctcgggcg tcagctccga
    18721 gccggcacgc tcgtgaacca tcgtcaggat gcgctcgcgc aactgcggca ccgcggtgtt
    18781 gctgcgcagg taatcatcga ggatggcgtc ccggtcgacg cccagcgact cgagcaccac
    18841 ggccaccgtg aagccggtgc ggtccttgcc tgcgaagcag tgcgtaatca ccgggcgtcc
    18901 gcttgccagc agcgagatca cttgacgtac cgcatttctc gcgccgccaa gggtcgggaa
    18961 gcggcggtac tcgtcggtca tgtaccgcac ggccgcctcc gccggcggct cgtcgtccgg
    19021 ctgctctgtc agcatccggg tgaacgaatg ctcgtgtggc gcttcgccgt cggcgtcttc
    19081 ggccagatca gggaacggca gcggatggat ggcgacgtcg tcgtgcacgc ggccgggtcc
    19141 gcggcgctca acctcccgcg gcgagcgcag gtcggcgacg tcgaagacac ccagcgctcg
    19201 aagctcgtcg cgtccgtcgt cctccagagc gctcaactcg ctggaccgga agaacctgcc
    19261 cggtcgtaca ccggtggcgt cggccacatc gcggaagttc cacgctccct tcaggtctgg
    19321 cacgattagg tgaccgccgc gacgaaagcg cttttctccc ggccgatttc ggcgcgagcg
    19381 atggtgcgca tatgcacctc gtcgggcccg tcgaagatcc gcatcgcccg atgccaggag
    19441 tacagctgcg ccagcggggt gtcgtcgctg acgcccgccg cgccgtgcac ctggatggcg
    19501 cggtcgataa cgtcgcaggc caccttcggt gctaccgcct tgatctggct gaccagcagg
    19561 tgggcggctt tgttgccgtg ctggtcgatg gtccaggccg ccttctcgca caacagcctg
    19621 gcctggtcga tctcgttgcg gcacttcgca attgactcgc gcactactcc ctgctcggca
    19681 agtggtttgc cgaacgccca ccggttgcgg gcgcggtcga ccatcaacgc cagcgcacgc
    19741 tcggcggcgc ccatggcccg caagcagtgg tggatgcggc ccggtcccag ccgcgcctgt
    19801 gcgattgcga agcccgatcc ttcctcgccc agcaggttct ccgtcggcac gcggaccttg
    19861 tcgtagatga cctcacagtg cccgtgctgg tcttgccagc cgaacaccgg cgtggaacga
    19921 aggactgtga cccctggcgt atctatgggc accagcacca tcgactgctg ctggtgactg
    19981 gcagcgtcgg ggttggtgcg gcccatcacg atcaggatct tgcagcgcgg gtcggacgcg
    20041 ccggacgtcc accacttgcg cccgttgatg acgtagtcgc tgccgtcgcg gacgatcgag
    20101 gtctctatgt tgcgggcgtc gctggacgcc acggcgggct cggtcatcga gaaggcgctg
    20161 cggatctcgc cggccaacag cggctcgagc cactgcttgc gctgctgctc ggtggcgaac
    20221 aggtgcaggg tctccatgtt gccggtgtcg ggcgccgcgc agttggtggc ttccggcgcg
    20281 atctccatgc tccagcccgt caactccgcc aacggcgcgt agtcggtgtt cgtcagcccc
    20341 gacaccgacg ggaggaacag gttccacagc ccgcgctcgc gggcgagctt cttcagctcc
    20401 tcgatgaccg gcggcacgct gtggtcgtcg ggacccctct cgaggcggta gcgctcgtat
    20461 tcggcctcgg ccgggaacac gaactcggtc atgaaagcgg acaaccgctt gtgatagtcc
    20521 tgcgccttgg ccgacatcgc gaaatccatg atcgtcacac taagccacca cggactccgt
    20581 tccgtcggcc gtaccggcgt gcgaagcttc atgccatggc agcttcggca cgcaggtgat
    20641 ggctgaacac acatgagcag cacagatctc tggcgggcgc gcaggcggca ccacacgcgg
    20701 tcgctgagga ttgcggcggt ccttcgcctc gccgtcgtga tcgtcatggt cgctgccatg
    20761 ctcctcggca cggctcacgc cgactggccc aagcagagcg tgttgctggt cgtgtatgga
    20821 ctcatggccg tatgcgttgc ggtgcttgca ttttcgcgcg tcggcgtatc agtgatcggg
    20881 ctgcggcagc ggttcgtctt cgccttcgcc gacgtgggtt tcttgttcgc gttccagttg
    20941 ttgtcgacgg gtggctacat ccccctggtg ctaatggcat tggtaccgct gatcgttgtc
    21001 ccgcagctgt cgtggcggca ggccgtcgtc gtcctgtccg tcagcgtcgc ggcattcgtg
    21061 tatgccgtgt tgcaagacga cgtgatcccc gtgcagctgg gctggcggga ttcggtcttt
    21121 ctcttcgtca tgtacggatt cctctgttgc acaggcctac tcgtagccta tgtcgaggag
    21181 caccatgtcg acgagatcgc cgcgctcacc gcgtcccgcg aagagttgct cggccagatc
    21241 atgacggcgt ccgagaccga gcgtcgccgg atttccgagg cgattcacga cgggccgctt
    21301 caggacgtac tcgtggctcg tcaggagatc ggcgagcttg ctgcgtcgtc gcgctcggaa
    21361 gaactcgacc gtgcgatggt ctgcctgcgc gatgcgtccc agcggttgcg agacgcgacg
    21421 ttcgaactgc atccggcagt gttggagcag gtcggcttgg gcgcggcggt agagcaattg
    21481 gcgacgttca cggcgacccg cgcccgcatc gccgtcgaca ccaacatcga ttaccccgca
    21541 caacagccga tcgatcccat catgttcggt gttgtccgcg agctgctgtc caatgtcgtg
    21601 cgccattcac aagcgagccg ggcctggatc acccttgtcg tcaccgactc gatgtgccgg
    21661 ctagacgtcg ccgacaacgg aatcgggatg acggacgaac gggctgcccg tcggctcgca
    21721 gaggggcata tcggcctggc ctctcaccgg gcgcgggtcg aggcggcggg cggaaagttc
    21781 acgatcgccg acgaagaagc cggaacccat gtgcgcgtgg agttgccgct tcccgactga
    21841 tcactccaac agcttgcggc gcatcgcttc ggcgacggcg gcgccgcggt caccgacacc
    21901 tagtttctcg tacagccgct gcacgtgcgt cttcaccgtg gagggcgcca gatagagctg
    21961 ggcggcgatc gcggggatgc tcttgccctc ggagatgccg gcgagcacct cccgctcgcg
    22021 cggcgtcagc accgggcccg tcggctcggc gcgtcggcgg atctcgccgg caagacccgc
    22081 ggcgacactg ggcgacacca cgtctcggcc cttcgcgcac gccagcacgg cgttgacgat
    22141 ctccgtcttg ttcgcctcct tgggcaggaa cccggccgcg ccttcttgca gcgcctgaaa
    22201 gacgatcgcc gactcgtcgt gtgcggacag gatcagcaca cgtgtcggca gttcgtcgcg
    22261 gcgtaccgcg gtgacgacct gggcaccgtc caaaccgggc attcggaagt cgagcagcgc
    22321 cacgtcgggc cgctgggtcc tgatcatttc gagagcggtg gttccgtctt cggcttcgcc
    22381 gacgacgtcc accgagccgc tcgcggtcag cgcccgcgcg acgccttcgc gcacgagagg
    22441 gtgatcgtcg gcgaccacca cgcgcacttt ctcgttcgct gccgggccgg gcatggtccc
    22501 agcttggcac aacggtgccc tgcgtatacc ccaatcccgc cggatggggg acgccgcatt
    22561 ccgtcgatgc atgggccgaa accatccgat cgacgcgacc gcgtctgcgc cgcacagtag
    22621 taccgggcgt tgaatctcga cgcgagcggc gtaaggcgaa agggcattga tgacaacaat
    22681 gctcgaaacc ctcgtctcgc ggggtaggcg tgcttctcaa tgccttccgc aatgacgacg
    22741 gggccttccg caatgacgac gggcctgcct cgactgactg gtcctttgag ggtctgcgga
    22801 tgatgaccgt cgggggcgac gcgctcgatc ccgggccagc agcaacggat caaatgcgca
    22861 gtgctgcaac ggcttctgcg cggaatggcc ctacgagatc caattgctgt ccgctgacca
    22921 gctgagtcac aagtgagcga ggctacttcg tcaatgcccg cgcgatgacg aggcgctgaa
    22981 tctggttggt gccctcgaag atctgcatga tcttggcttc gcgcatgtag cgctcgacgc
    23041 ggtagtcgcg ggtgtagccg acgccgccga acacctgcac ggcgtcggtg gtgaccttca
    23101 tcgcggcgtc ggtggcgatc agcttggcga cgctggcctg cgtcgaatag gggcggccgg
    23161 cgtcgcggcg gcgggcggcg tcgagatagg tggcccgggc actggtcacc gcggcggcca
    23221 tgtcggccag cacgaacccc aggccctggt ggtcaatgat cttgcggccg aatgtcgttc
    23281 gctcgttggc gtaggcggcg gcctcgtcga gtgccgcctg cgccaagccc accgcgacgg
    23341 ctgcgatgcc cagccggccg gaatccagcg cggagaacgc gatctgcaaa ccttggcctt
    23401 ctccaccgat gagccggtcg gcgtcgaggt gggcgtggtc gtaaaacgcc gacgtggtcg
    23461 gcacggcgtg cagccccatc ttctcctcgg gcttaccgaa cgacagcccc ggcaggcttg
    23521 cggggatcag gaagcaggag atgccgcggc tgccttctcc ggcgcgggcg aacaacatgt
    23581 agaagtcggc ccgcccgccg tgggtgaccc acgacttcga cccgttaatg acgtagccgc
    23641 cgtcgctgcg cttggccgcg caccgcagcg cggccgcgtc ggatccggcc tgcggctccg
    23701 acaggcagta cgcgccgatc tgctctccgg agagcatgcc gcgcagccag cgctgctttt
    23761 ggtcgtccga cccgaacgtc agcagcggat gacacgacag gctgtgcaca ctgaccgcca
    23821 ccgcgatcgc ggcccagcgg gcggcgatct cctcgagtac ctgcagatac acctcgaacg
    23881 gctgcccgcc gccaccccac tcctgcggct gcggaaggct cagcagaccg gcggcgccga
    23941 gttggggaaa taccccctcg ggatatgatt ccgctttctc gtgggcgtcg acaatcggcg
    24001 tcaacacctt gtcggcgagg tcgcgggcaa gaacgatgag gtcagccgct tcgtcggtag
    24061 gcagcaggcg gtcgacaggc atgcagcatg cttaccgcag aagcggcaga ccggggaagt
    24121 tgagatgaca gacagccaga cgagccctga tgtcgccggc cgggcggctc ggggtcctga
    24181 tgtcgccggc caggcggctc cgaccatcgg ggtggttcgg gagtccggtg acggtgagcg
    24241 cagggtggcg ctggtgccca aggcggtcca tgcgctcgct ggccgcggcc tggcggtggt
    24301 agtggagtcc ggtgccggcg acggtgcgct gctgcccgac gacctgtaca ccgaggccgg
    24361 cgcgacgatc ggcgacgcat ggggtgccga cgtggtggtg aaggtggccc cgccgaccgc
    24421 cgacgaagtg gggcggctgc gcagcggcca gagcctgatc ggattcctgg caccgcgcaa
    24481 cgccgacaat caggtcgacg cgctgacgaa ggcgggcgtg caggcgttcg cactggaagc
    24541 catcccgcgg atctcccggg cgcagacgat ggacgcgctg tcgtcgcagg cgaacgtcgg
    24601 cgggtacaag gcggtgctgg tggcggccag cgaggcgacc cggttcttcc cgatgctcac
    24661 caccgcggcc ggcacggtga agccggcgac tgtgctggtg ctgggcgtcg gggtggcggg
    24721 cctgcaggcg ctggccacgg ccaggcgact cggcgcccgc accagcggct acgacgtgcg
    24781 tcccgaggtc gccgaacagg tgcggtcggt gggcgcgcag tggctggatt tgggcatcga
    24841 cgccgccggc gagggcggct acgcgcgtga gctg
//
LOCUS       DASWBF010000324         5915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1144013,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000324 DASWBF010000000
VERSION     DASWBF010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144013"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..619
                     /locus_tag="VF874_11835"
     CDS             116..619
                     /locus_tag="VF874_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9176724.1"
                     /translation="MSSSPDKRDPIAQARANWQRSGWDDVADGMVAVTSVMRAHQILL
                     ARVENALRRYDLSFSRFELLRLLAFSRTGALPIGKASDVLQVHVTSVTHAIRRLESAG
                     LVERVPHPTDGRTTLVQITPLGRSTVEDATVTLNDEVFADIGMSAEESRALAASIDTL
                     RRNSGDF"
     gene            complement(656..1894)
                     /locus_tag="VF874_11840"
     CDS             complement(656..1894)
                     /locus_tag="VF874_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX9176725.1"
                     /translation="MTQPRAVRDLVDADRELALLRELIQAASSGPGVEPLAAAAARMI
                     TAATGTDVCFVHVLDDTNRSLTLTGATPPFDSEVGKIRLPLGSGISGWVASHREPVVI
                     SHDKESDPRYMPFQSLRGRDFTSMVSVPMETDPGGLVGVLNVHTVERREFTDRDVQLL
                     LVIGRLIAGAMHQARLHRQLVARERAHENFVEQVIEAQELERRRLAGDIHDGISQRLI
                     TLSYWLDAARRAVGDNPDAVAEQLEKARELVELTLQEARAAIGGLRPPVLDDLGLAGG
                     LASLARSIPQLDLEVELADSRLPDHIEIALYRIAQECLQNVVKHAKASTAKLTFAVDG
                     ATARLEIVDDGVGFDTFEHPLGGDEMGGYGLLSMAERAEIVGGRLNIRSRPGAGTAVT
                     ATIPLPTPLAESETRAVKPG"
     gene            complement(1891..2556)
                     /locus_tag="VF874_11845"
     CDS             complement(1891..2556)
                     /locus_tag="VF874_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9176726.1"
                     /translation="MPTAAGPVRIVLVDDHEMVIEGLKAMLAAFSDRVQVVGQAVGAE
                     RALPVIEQLDPDIVLCDVRMQGSSGLDLCQALRQRNPERKIVMLSVYDDEQYLFQAMR
                     VGASGYLLKSISSDELVRQLEFVRSGETAIDPGMAARAVDTAARIQRDEFWPGARHGL
                     SQRESEILSYVVSGLSNRGIASKLVIGDETVKSHLRSIYRKLGVSDRTGAVATALREG
                     IYQ"
     gene            2681..3202
                     /locus_tag="VF874_11850"
     CDS             2681..3202
                     /locus_tag="VF874_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX9176727.1"
                     /translation="MRGQTLARRTFWWDRTSDADVDPLRAVIFDFDDAVADIDHDGEL
                     TPRSGLIDLVMSLFFAGVWVGVVSTKRRAEVEPLVRELIGDGLVETIVTSDDAESTDS
                     TDLYRLALWEFGVGPESALAVVGSSAGLRTASDAGLATMVVSTDYEDFSNAAACERVH
                     RRWWTERKRAAAA"
     gene            complement(3199..4077)
                     /gene="mmsB"
                     /locus_tag="VF874_11855"
                     /pseudo
     CDS             complement(3199..4077)
                     /gene="mmsB"
                     /locus_tag="VF874_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
     gene            complement(4100..5272)
                     /locus_tag="VF874_11860"
     CDS             complement(4100..5272)
                     /locus_tag="VF874_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176728.1"
                     /translation="MFRLDDDERVITDTAAAFAAKRLAPHALEWDAANHFPIDALREA
                     AELGMAAIYCGEDVGGSGLRRLDAVRIFEQLAAADPTTAAFLSIHNMCAWMVDRYGTH
                     EQRKTWVPKLASMETVASYCLTEPSAGSDAAALRTKAVRDGTDYVLDGVKQFISGAGA
                     SDVYVVMARTGFQETEPVHRQISAFLVENGAAGLSFGAQEQKMGWHAQPTAQVILEGV
                     RVPADAMLGGTDGEGTGFGIAMSGLNGGRINIAACSLGGAQAAYDKAVAYVRDREAFG
                     ASLLDEPTIRFTLADMATTLESSRMLLWRAATALDDDDADKVALCAMAKGYVTDACYD
                     VADQALQLHGGYGYLREYGLEKIVRDLRVHQILEGTNEIMRVVIGRAEAARVKART"
     gene            complement(5286..>5915)
                     /locus_tag="VF874_11865"
     CDS             complement(5286..>5915)
                     /locus_tag="VF874_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9176729.1"
                     /translation="ERLRARLIDRINNLRVGHSLDPKADYGPLVTEAALARVKDYIQR
                     GVDAGAEAVIDGRERASDDLQFGDANLEEGYFIGPTLFDHVTPDMSIYTDEIFGPVLC
                     IVRAKDYEEALRLPSEHEYGNGVAVFTRDGDTARDFVSRVQVGMVGVNVPIPVPVAYH
                     TFGGWKRSGFGDLNQHGPASIQFYTKVKTVTQRWPAGTHGAEFVIPTMK"
BASE COUNT          919 a         2003 c         1974 g         1019 t
ORIGIN      
        1 gagggggcat tgtttccgcg cgcgtcatca attgatgaca ttctcgctta ttaaattaac
       61 tgcgaccccg aggtggccat gccgtttcta cccccacagt gcggtatgaa gaggcgtgtc
      121 ctcctcgcca gacaagcgtg accccatcgc gcaggcccgt gccaactggc aacggtcggg
      181 gtgggacgac gtcgccgacg ggatggtcgc cgtcacgtcg gtgatgcggg cgcatcaaat
      241 cctgctggcg cgggtggaga acgcgctgcg gcgctacgac ctgagcttct cccgattcga
      301 gttgctgcgc ctgctggcgt tcagccgcac cggcgcgttg ccgatcggca aggcgtccga
      361 cgtgctgcag gtgcacgtca ccagcgtcac gcacgcgatc cggaggctgg agtcggcggg
      421 actggtcgag cgggtgcccc acccgaccga cggccgcacc acgctggtgc agatcacccc
      481 cctgggacgc tcgaccgtgg aggacgcgac ggtgacgctg aacgacgagg tgttcgccga
      541 catcggtatg tccgccgagg agtcgcgggc gctggccgcg tcgatcgaca cgctgcgccg
      601 caactccggc gacttctagc ggcttcttgc gccgaaaccg aattccaggt tgtggttagc
      661 ccggtttcac agccctggtt tcactttcgg cgagcggcgt cggcagcggg atcgtcgcgg
      721 tgaccgcagt cccggcaccg ggccgggacc ggatgttgag ccggccgccg acgatctcgg
      781 cgcgctcggc catcgacagc agcccgtagc cgcccatctc gtcgccgccg agcggatgtt
      841 cgaacgtgtc gaaacccacg ccgtcgtcga cgatttcgag ccgcgcggtg gcgccgtcca
      901 ccgcgaatgt gagcttggcc gtgctggctt tggcgtgctt gaccacgttt tgcaggcact
      961 cctgcgcgat gcggtacagc gcgatctcga tgtggtcggg cagccggctg tcggcgagct
     1021 ccacctcgag atcgagctgg ggaatcgaac gggcgaggct ggccagcccg ccggccaggc
     1081 ccaggtcgtc gagcaccggt ggccgcagcc cgccgatcgc ggcgcgggcc tcctgcagcg
     1141 tcagctcgac cagctcgcgg gccttctcca gctgttcggc caccgcgtcc gggttgtcgc
     1201 cgactgcgcg gcgcgccgcg tccagccagt aggacagcgt gataagtcgc tgcgagatgc
     1261 cgtcgtggat gtcgccggcg aggcggcgac gctcgagctc ctgcgcctcg atcacctgct
     1321 cgacgaagtt ctcgtgggcg cgttctctcg cgacgagctg gcggtgcagc cgcgcctgat
     1381 gcatcgcgcc tgcgatgagc cgtccgatca ccagcagcag ctgcacgtcg cggtcggtga
     1441 actcgcgccg ctcgaccgtg tgcacgttca gcaccccgac caacccgccc gggtcggtct
     1501 ccattggcac cgacaccatc gacgtgaaat ctcgcccgcg cagcgactgg aacggcatgt
     1561 agcgcggatc ggactccttg tcgtggctga tcaccactgg ttcgcggtgg ctggcgaccc
     1621 atcccgagat gccagagccc aacggcagcc gaatcttgcc gacctcgctg tcgaacggcg
     1681 gcgtcgcgcc ggtgagcgtc aacgatcggt tggtgtcgtc gaggacgtgt acaaagcaca
     1741 cgtcggtacc ggtcgcggcg gtgatcatgc gcgcggcggc cgccgctagc ggttcgaccc
     1801 ccgggccgct ggaggccgcc tgaatcaact cgcgtagcag cgccagttca cggtcggcgt
     1861 cgacgagatc gcggacggcg cgtggttgtg tcattggtag atgccttccc gcagggcggt
     1921 ggcgaccgca ccggttcggt cgctcacgcc gagcttgcgg tagatcgatc gcagatggct
     1981 cttgacggtt tcgtcgccaa tcacgagctt gctcgcgatg ccccggttcg acagcccgct
     2041 caccacgtag gacaggatct cgctttcgcg ctgcgacagc ccgtggcggg cgccgggcca
     2101 gaactcgtcg cgctgaatgc gcgcggcggt gtcgacggcg cgagctgcca tgccggggtc
     2161 gatcgcggtt tcgccgctgc ggacgaactc gagctgacgc accagctcgt cgctgctgat
     2221 gctcttgagc aagtaccccg acgcgccgac ccgcatcgcc tggaagaggt actgctcgtc
     2281 gtcgtacacc gagagcatga cgatcttgcg ctccgggttg cgttggcgca gcgcctggca
     2341 caagtcgagg ccgctggagc cctgcatgcg cacgtcgcac agcacgatgt cagggtcgag
     2401 ctgctcgatg acggggagcg cgcgctcggc gcccaccgcc tggccgacga cttgcacgcg
     2461 gtcggagaac gcggccaaca tggccttgag cccctcgatc accatttcat ggtcgtcgac
     2521 cagcacgatt cgcacaggtc cggcagccgt tggcatggga tcacgataga gccggctcac
     2581 ccggtccgtt acgcagcagg ctgaagtggg acaccaatag cctgcccaat cccccctgtg
     2641 ggggatgcct cgacgcgcga gtcggcagat gctgtggtgc atgcgggggc agacacttgc
     2701 acggcggacg ttctggtggg accggacgtc agacgccgac gtcgaccctc tgcgcgcggt
     2761 gatcttcgat ttcgacgacg cggtggccga catcgatcac gacggagagc tcacgccgcg
     2821 gtccggcctg atcgatctgg tgatgagctt gttcttcgcc ggcgtgtggg tcggggtggt
     2881 cagcaccaag cgccgggccg aggtggagcc gctggtgcgg gagctgatcg gggacggtct
     2941 cgtcgagacg atcgtgacga gtgacgacgc cgaatccacc gacagcaccg acctctaccg
     3001 gttggcgttg tgggaattcg gcgtaggccc ggaaagcgcc ttggccgtgg tgggttcttc
     3061 ggccggctta cgcacagcca gcgacgcagg cttggccacg atggtcgtgt cgacggatta
     3121 cgaagacttc agtaacgcgg cggcgtgtga acgcgtacac cgccgttggt ggaccgagcg
     3181 caagcgggca gccgccgctt agctcttgcg cagcatctcg atgacggcgc tgaagtcccg
     3241 gtccgagtgg tcagaggcga acttagcgta gatttcggcg gcgtggctgc ccagcggggc
     3301 cgccgaaccc gttgacctga ccgcggccat cgccagcccc aggtccttgt tcatcagcgc
     3361 ggcggcgaac ccgggcttga agtcgttgtt ggccggagac gtcggaaccg gtcccggcac
     3421 aggacagttc gtgtgcaccg cccagcagtt acccgtcgcg ccggtaacca cgtcgaacag
     3481 cgactgagcg gacaacccga gcttctcagc cagcaggaac gcctctccga ccgcgatctg
     3541 ctgaaccgcc agcagcatgt tgttgcagag cttggcggcc tggccagtgc ccgatgcgcc
     3601 gcagtgaatg atcttgcccg ccaacggttc cagcaccggc ctggcgcggt ccacagccgc
     3661 ctcgtcgccg ccgaccatga acgccaacgt gcccgcagtc gcccccttga caccgccgga
     3721 caccggagcg tcgagttgcg cgaaaccccg ctcgacggcc tgcccgttga tcgagcgggc
     3781 gtcgtcgacg gagatcgtgg aggtgtcgat gaacagcgcg cccggcgtgg cggcggccaa
     3841 aacctcggcg taacaagcct tcacgatgtc accattgggc agcgacgtga tcaccacatc
     3901 ggcgtcggcg acggcctctg ctccagtgtc gaacatctgg gcgccctttt cggcggccgt
     3961 tgccttcagt acgggcactg ggtcgaagcc gcgtacggtg tgtcccgccg ctaccaggtt
     4021 cgccgccatc gggccgccca tgttgcccag gcccagaaat gcgatcgtcg tcatctgccg
     4081 gcttcctcca tcttgctcct cacgtccgcg ccttcacccg ggcggcctcg gcgcggccga
     4141 tcaccacccg catgatctcg ttggttccct cgaggatttg gtgcacccgc aagtcgcgga
     4201 cgatcttctc cagtccgtac tcgcgcagat agccgtatcc gccgtgcagt tgcagcgcct
     4261 gatcagctac gtcgtagcac gcatcagtga cgtagccttt ggccatcgcg cacagtgcga
     4321 ccttgtccgc gtcgtcgtcg tccagcgcgg ttgccgcacg ccacaacaac attcgcgacg
     4381 actccaacgt ggtggccatg tcggcgagcg tgaagcggat tgtgggctcg tcgagcaggc
     4441 tggcaccgaa cgcctcacgg tcgcgcacgt acgccacggc cttgtcgtag gcagcctgtg
     4501 cgccgcccag cgagcacgcc gcgatattga tccgtccgcc gttgaggccg ctcatcgcaa
     4561 tcccgaaccc ggttccctcg ccgtcggtgc cgccgagcat cgcgtcggcc ggcacccgga
     4621 ccccctcgag gatcacctgg gcggtgggct gagcgtgcca gcccatcttc tgctcctgtg
     4681 caccgaaact cagcccggca gcgccgtttt cgacgaggaa cgccgagatc tgacgatgga
     4741 ccggctccgt ctcttgaaag cccgtgcggg ccatcacgac gtagacgtcc gacgcgcccg
     4801 ccccggagat gaactgcttg accccgtcga gcacgtagtc ggtcccgtcg cgcaccgcct
     4861 tggtgcgcag cgccgccgcg tcggagccgg ccgagggttc ggtgaggcag tagctggcga
     4921 cggtctccat cgaggcgagt ttcggcaccc aggtcttgcg ttgctcgtgg gtgccgtagc
     4981 ggtcgaccat ccacgcgcac atgttgtgga tcgacaggaa cgcggccgtg gtcgggtcgg
     5041 cagcggccag ctgttcgaag atccgtaccg cgtccaggcg ccgcagcccg ctgccgccca
     5101 cgtcctcgcc gcagtagatc gcggccatcc ccaactcggc ggcttcacgc agcgcgtcga
     5161 tagggaagtg attggcggcg tcccactcca gggcgtgcgg agcgagccgc ttcgcggcga
     5221 acgcggcggc cgtgtcggtg atgacgcgct cgtcgtcgtc cagccgaaac atcatgcagc
     5281 cggacctatt tcatcgtcgg gatgacgaac tcggcaccgt gcgtccccgc gggccaccgc
     5341 tgggtcacgg tcttgacctt cgtgtagaac tggatcgacg ccgggccgtg ctgattgaga
     5401 tcgccgaagc ccgaacgctt ccagccgccg aacgtgtggt aggccaccgg taccggaatc
     5461 ggcacgttga caccgaccat cccgacctgt acgcgggaca cgaagtcgcg ggcagtgtcg
     5521 ccgtcgcggg tgaaaaccgc tacgccgttg ccgtattcgt gctcactcgg caaccgcagc
     5581 gcctcctcgt aatccttcgc gcgcacgatg cacagcacgg gaccgaagat ctcgtcggtg
     5641 tagatcgaca tgtccggggt gacatggtcg aacagggtgg gaccgatgaa gtagccttcc
     5701 tcaaggtttg cgtcgccgaa ttgcaggtcg tcgctggcgc gttcgcggcc gtcgatcacc
     5761 gcctcggcgc cggcgtccac accccgctgg atgtagtcct tgacccgcgc gagcgccgcc
     5821 tcggtgacca gcggtccgta gtcggccttg gggtcgaggc tgtggccgac gcgcaggttg
     5881 ttgatcctgt cgatcagcct ggcgcgcaag cgttc
//
LOCUS       DASWBF010000325         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_760825,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000325 DASWBF010000000
VERSION     DASWBF010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760825"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VF874_11870"
     CDS             <1..157
                     /locus_tag="VF874_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEX9176730.1"
                     /translation="GPLYAPKTIELCSPDDTICNGTPGGQPSIAHALYSVNGMTNEAA
                     TFAVSRL"
     gene            193..834
                     /locus_tag="VF874_11875"
     CDS             193..834
                     /locus_tag="VF874_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEX9176731.1"
                     /translation="MSSVTVRFLTAAVVTTGAFLTTVVPVPSATAAPCPDIEMVFARG
                     TTEPPGVGGVGQAFVDSLRGQVGGRSVGVYPVNYPATNDFGSSIPAGANDASAHVQSM
                     AANCPSTRMVLGGYSQGAAVMDVITAAMPPQVANNVAAVAVFGNPSPAGSLSRRLWSG
                     SLAGIGPLYVPKTIDMCMPGDPICTDGVSMGPHGQYVQSGMTSQAATFAAGRL"
     gene            complement(890..1264)
                     /locus_tag="VF874_11880"
     CDS             complement(890..1264)
                     /locus_tag="VF874_11880"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEX9176732.1"
                     /translation="MKNAAAIILVAAGAVVARALGAAAPANAQADKYVAISYSPETGA
                     YGWGNNYNDLDSARIRSLSECQNYGGNHCVFLTWSKNGCAALAVNGDSYYGWYGATRS
                     EAEQQALQRNGGGHILASQCAT"
     gene            1372..1857
                     /locus_tag="VF874_11885"
     CDS             1372..1857
                     /locus_tag="VF874_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppP/LprE family lipoprotein"
                     /protein_id="HEX9176733.1"
                     /translation="MRVRRLTAVLLMFGFVAAGCGWKPPSAPPPSPDKCTATDGPTAD
                     TVTSAVDGLSPAAGGAWREVGRGHTANCRLYWVQVSAGTDPAAPQHLLFFDRNTPIGT
                     ATPQPKPYTTVLNSGENTVIVQYQWQQGSDAPGRPTGIGQVRFQLGNDGKLKALDPIP
                     N"
     gene            complement(1858..>2858)
                     /locus_tag="VF874_11890"
     CDS             complement(1858..>2858)
                     /locus_tag="VF874_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9176734.1"
                     /translation="KAPDDDSEVLLMDYLVHQRRLLPLIAKSYALQFAQNELVAKLDE
                     VQSTEIPDPEEQRELESRAAGLKAANTWHASRAIQEAREACGGAGYLAENRLIALRGD
                     TDVFTTFEGDNHVLTQLVAKELLTAYADDIKSMSPVEWVRFAANTVGDRVVKRTAAQP
                     IIQTLLDTRQDNEEEGSLFNRGTQLQMFEDREEYMLSRVARRLQGRSKELDAFDAFNE
                     VQDHVLHAASAHIDRVILEAFVAGIESCEDEQAQRVLSTVCDLYALSVIEEDKAWYVE
                     HRYLSSERAKAVTRGINERCRTLRPYAEALVDGFGIPEQLRDVPMLHPERIGDNEE"
BASE COUNT          462 a          961 c          923 g          512 t
ORIGIN      
        1 cggcccgcta tatgccccca agaccatcga gttgtgtagt cccgacgaca cgatctgtaa
       61 cggcactccc ggtgggcagc ccagcatcgc gcacgcgctg tattccgtga acgggatgac
      121 caacgaggcc gcgacgttcg ccgtgagtcg gctctgacgc aacaaaatga cagcgccgag
      181 cgatatacca gcatgtcgtc cgtaacagtt cgtttcctca cggctgcggt ggtgacgacg
      241 ggggcgttcc tcaccacagt cgtgccagtt ccttcggcca ccgctgctcc atgccccgac
      301 atagaaatgg tgttcgcccg cggcaccacc gagccacccg gtgtcggcgg ggtcggccag
      361 gcgttcgtcg actcgctacg cgggcaagtc ggtgggaggt cagtcggggt ctacccggtc
      421 aactaccccg ccaccaacga cttcggcagc tccatccccg ccggtgccaa tgacgcgagc
      481 gcgcacgtcc agtccatggc cgcgaactgt cccagtaccc ggatggtgct cggtggctat
      541 tcccaaggcg cggccgtcat ggacgtcatc accgcggcga tgccacccca ggtggctaat
      601 aacgtcgccg cggtcgccgt tttcggaaat ccatcgccgg cgggctcact ctcgaggaga
      661 ctgtggtccg gctcgcttgc cggaatcggc ccgctgtacg tccccaagac gattgacatg
      721 tgcatgcccg gcgatccgat ttgcaccgac ggcgtgagca tggggccgca cggtcagtac
      781 gtgcagtccg gaatgacaag tcaggcagcg actttcgctg cgggccgact ctaggtcgag
      841 cgaatcggcc cgccacccgg aaagggcgac gggccgatta gcggtccgct taagttgcgc
      901 actgcgacgc gaggatgtgg ccgccgccgt tgcgctgcag cgcctgctgc tcggcctccg
      961 agcgagtcgc gccgtaccag ccgtagtagc tgtcgccgtt gacggctagg gccgcacaac
     1021 cgttcttcga ccaggtgagg aacacgcagt ggttgccacc gtagttctgg cattcgctca
     1081 gcgagcggat tcgcgcgctg tccagatcgt tgtagttgtt cccccagccg taggcgccgg
     1141 tctccggcga gtacgaaatc gcgacgtact tgtcggcctg cgcattggcg ggtgccgcgg
     1201 cgcccagcgc tctggcgaca accgcgccgg cggcaaccag gatgatggca gcagcattct
     1261 tcatggtgtt cgccttcagg ttctcgcagc ccccggtggg ttgcggtcac ctgctaggag
     1321 cgaccagtcg ctgaaacggg gacacttttt tgagcgccta gggtgggttt catgcgcgtt
     1381 cgccggctga cggccgttct gctgatgttc ggattcgtcg ccgccgggtg cggctggaag
     1441 cctccgagtg caccgccgcc atcacccgac aagtgcaccg cgaccgacgg tccgacggcc
     1501 gacaccgtga ccagcgccgt cgacggcttg tcaccggcag ccggcggcgc gtggcgcgag
     1561 gtgggccgcg gtcacaccgc gaactgccgc ctgtactggg tgcaggtgag cgccggcacc
     1621 gacccggccg caccgcagca cctgctgttc ttcgaccgca acaccccgat cggcaccgcg
     1681 acaccgcaac ccaagccgta cacgaccgtg ctgaactccg gcgagaacac ggtgattgtg
     1741 cagtaccagt ggcagcaggg cagcgacgcc ccgggtcggc cgactggaat cggtcaggtc
     1801 cgtttccaac tcggcaacga cggcaagctc aaggcgctgg acccgatccc gaactagtta
     1861 ctcctcgttg tcgccgatgc gctccggatg cagcatcggc acgtcgcgca gctgctcggg
     1921 aatcccgaaa ccgtcgacga gcgcctcggc gtagggccgc agcgtccggc agcgttcgtt
     1981 gatgccgcgg gtgacagcct tcgcccgctc ggaggacagg tagcggtgtt cgacgtacca
     2041 cgccttgtcc tcctcgatca ccgacaacgc atacaggtcg cacaccgtgg agagcacccg
     2101 ctgggcctgt tcgtcctcac aagactcgat gccggcgacg aacgcttcca ggatgacgcg
     2161 gtcgatgtgc gcggacgccg cgtgcagcac gtggtcctgc acctcgttga acgcgtcgaa
     2221 cgcgtccaac tccttcgatc tgccctgtag ccgtcgggcg accctggaca gcatgtactc
     2281 ctcgcggtct tcgaacatct gcagctgagt gccacggttg aacaggctgc cctcttcctc
     2341 gttgtcctgt cgcgtgtcca gaagtgtctg aatgatcggt tgtgccgccg tgcgtttgac
     2401 cacccggtcg ccgacggtgt tggccgcgaa ccgaacccac tcgacggggc tcatgctctt
     2461 gatgtcgtcg gcgtaggcgg tcagcagctc cttggcgacg agctgggtga gcacatggtt
     2521 gtcgccctcg aaggtggtga acacgtcggt gtcgccgcgc agcgcgatca gccggttctc
     2581 ggcgagatag cctgcgccgc cgcacgcttc gcgcgcctcc tggatcgctc ggctggcatg
     2641 ccacgtgttg gcggccttga ggccggccgc gcgcgactcc agctcacgct gttcctcggg
     2701 gtcggggatc tcggtggact ggacctcgtc gagcttggcc accaactcgt tctgtgcgaa
     2761 ctggagcgcg taggacttcg cgatcagcgg cagcagccga cgctgatgga cgaggtagtc
     2821 catcagcagc acctcggagt cgtcgtcggg agccttga
//
LOCUS       DASWBF010000326         9300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1218617,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000326 DASWBF010000000
VERSION     DASWBF010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218617"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..248)
                     /locus_tag="VF874_11895"
     CDS             complement(90..248)
                     /locus_tag="VF874_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176735.1"
                     /translation="MNALEQLARDVRGFAMEVRRLGYSVPGGHENRFIELSERMIRHT
                     DDLHRPSH"
     gene            complement(420..1856)
                     /gene="glnA"
                     /locus_tag="VF874_11900"
     CDS             complement(420..1856)
                     /gene="glnA"
                     /locus_tag="VF874_11900"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9176736.1"
                     /translation="MAEKTADDIFKMIADEDVEYVDVRFCDLPGIVQHFSIPASAFNE
                     DVFEDGLAFDGSSIRGFQSIHESDMMLLPDPETARIDPFRAAKTLNISFFVHDPFTRE
                     PYSRDPRNVARKAENYLTSTGIADTAYFGAEAEFYIFDSVRYDSSINSTFYEVDSESG
                     YWNAGEATELDGTPNRGYKVRPKGGYFPVAPYDHYVDLRDKMTTNLINAGFTLERGHH
                     EVGTAGQAEINYKFNTMLHAADDLLLFKYIIKNTAWENGKTVTFMPKPLFGDNGSGMH
                     AHQSLWKDGEPLFHDESGYAGLSDTARHYIGGILHHAPSLLAFTNPTTNSYKRLVPGY
                     EAPINLVYSQRNRSACVRIPITGNNPKAKRLEFRCPDSSGNPYFAFAAMLMAGIDGIK
                     NKIEPMAPIDKDLYELPPDEAANIPQAPTSLASVIDKLEEDHEYLTEGGVFTEDLIET
                     WIAYKREQEILPVQVRPHPYEFALYFDV"
     gene            1968..2399
                     /locus_tag="VF874_11905"
     CDS             1968..2399
                     /locus_tag="VF874_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9176737.1"
                     /translation="MLGRVALTDSGYAGESLGLPASGPRSLAGFGRRILALLLDWLIA
                     YGLAGLVMSFGWLTPQTLATAVLVIWFCLGVVSVRLFGFTPGQFALGLMVVSVDDRIH
                     VGAGRAAARGLLIALVVPPLFTDSDGRGLQDRLTSTAVVRR"
     gene            complement(2458..3210)
                     /locus_tag="VF874_11910"
     CDS             complement(2458..3210)
                     /locus_tag="VF874_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HEX9176738.1"
                     /translation="MAKTRDSAAIKAAKAEAKTARKAASKERRSQLWQAFNLQRKEDK
                     RLLPYMIGAFVLIVAASVAAGLFISGVAGLTLIPLGVVLGALVAFIIFGRRAQRSVYS
                     KAEGQTGAAAWALDNLRGKWRVTPGVAATGHFDAVHRVIGRPGVIFVAEGSATRVKPL
                     LAQEKKRAARLIGDVPIYDVIVGNGDGQVPLAKLERHLTRLPANITAKQMDSLESRLA
                     ALGTKSGMAQMPKGPLPTQAKTRNMQRTVRRR"
     gene            complement(3233..4156)
                     /gene="lipA"
                     /locus_tag="VF874_11915"
     CDS             complement(3233..4156)
                     /gene="lipA"
                     /locus_tag="VF874_11915"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX9176739.1"
                     /translation="MSRGPRKLLRLEVRNAQTPIERKPPWIKTRARMGPEYTALKGLV
                     RREGLHTVCEEAGCPNIYECWEDREATFLIGGEQCTRRCDFCQIDTGKPAELDRDEPR
                     RVAESVAAMGLRYSTVTGVARDDLPDGGAWLYAQTVRQIKELNPNTGVELLIPDFNGE
                     PDLLQQVFGARPEVLAHNVETVPRIFKSIRPAFGYQRSLDVLTAARDDGLVTKSNLIL
                     GMGETTDEVHTALTELKNAGCDIVTITQYLRPSVRHHPVNRWVHPDEFVELADFAKGL
                     GFAGVLAGPLVRSSYRAGRLYAEAVAGRTRA"
     gene            complement(4153..4815)
                     /gene="lipB"
                     /locus_tag="VF874_11920"
     CDS             complement(4153..4815)
                     /gene="lipB"
                     /locus_tag="VF874_11920"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX9176740.1"
                     /translation="MRPIRAGAAPIEVRQLGTVGYDEAWQLQRELVAARVAGGPDTLL
                     LLQHPSVYTAGKRTQPHERPLDGTPVVDTDRGGKITWHGPGQLVGYPIVGLADPIDVV
                     NYVRRLEDSLIAVCTELGLSVGRVEGRSGVWLPGTPQRKVGAIGVRVQRATTLHGFAL
                     NCDCDLDAFDAIVPCGIADAGVTSLTAELGRRVRVDDVVGRVADAVCAALDGRLALAS
                     MP"
     gene            complement(4828..5736)
                     /locus_tag="VF874_11925"
     CDS             complement(4828..5736)
                     /locus_tag="VF874_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX9176741.1"
                     /translation="MADALIVIAGSSGLIGSALVTALRASDNRVVRIVRRAPSNSDEL
                     HWNPQTGEFDPDALRGVDAVVNLCGAGVGDKRWSGAYKQQLRDSRIAPTEVISAAVVE
                     AGVQVLINASAVGYYGNTGDRVVDETAPAGTGFLAQLCEDWEAATLPAQQAGVRVVLA
                     RTGLVLSPGGGLLSRLRPLFSLGLGARLGNGRQYMPWISLEDEVRALLFAISHDVSGS
                     LNLTGPAPVTNAEFTAALGRALNRPTPLPMPAVVLRRLLGEFADEGLLAGQRAIPAAL
                     ERAGFAFHHNTIGEALSYAMSRTDVL"
     gene            complement(5740..7455)
                     /gene="sucB"
                     /locus_tag="VF874_11930"
     CDS             complement(5740..7455)
                     /gene="sucB"
                     /locus_tag="VF874_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEX9176742.1"
                     /translation="MAFSVQMPALGESVTEGTVTRWLKQEGDTVERDEPLLEVSTDKV
                     DTEIPSPAAGVLKKIVAQEDDTVEVGGELAVIGDEASGEEQTIDADGESGGDEQPAQE
                     SAEEKEEKPAEEPEAQPEPAAEEPEEPDAKPAAKSSSDGKSTSVKMPELGESVTEGTV
                     TRWLKKVGDKVEADEPLLEVSTDKVDTEIPSPVSGTLLSITAEEDDTVEVGGELAKIG
                     EPGESGEAPAEPKPEPEAKEEPKPEPQKEPAPAAASSNGDGTPYVTPLVRKRASENNV
                     DLAKVKGTGVGGRIRKQDVLAAAEEAKAPAKEEPKREPAKDPAAAAEKPTSALAHLRG
                     TTQKANRIRQITAKKTRESLQATAQLTQTHEVDMTKIVALRARAKNDFAEREGVKLTY
                     LPFIARAVIDALKIHPNVNASYNEETKEITYYDAEHLGIAVDTEQGLLSPVIHHAGDL
                     SLAGLARAIADIADRARSNNLKPDELSGGTFTITNIGSQGALFDTPILVPPQAAMLGT
                     GAIVKRPRVIVDKLGNESIGIRSVSYLPLTYDHRLIDGADAGRFLTTIKRRLEEGAFE
                     ADLGL"
     gene            7589..7927
                     /locus_tag="VF874_11935"
     CDS             7589..7927
                     /locus_tag="VF874_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX9176743.1"
                     /translation="MGFFDRFRGGRRGESDADIAADHRYLRDWARARTGVEAFVEPQT
                     SVTDVTVVLVAGDGEWTRRRVGGASGARRLGEQLRIPVYDVQRVGYPQRMRDYDARRR
                     AERRRAEEAE"
     gene            7924..>9300
                     /locus_tag="VF874_11940"
     CDS             7924..>9300
                     /locus_tag="VF874_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9176744.1"
                     /translation="MIVDNGDVRIAVYEHGNPDGPTVVMVHGWPDSHVLWDGVVPLLA
                     DRFRLITYDNRGVGPSSSPKSYREYTTAGLADDFAAVIDAVSPGRPVHVLAHDWGSVS
                     VWEYLARPEARTRVASFTSVSGPSQDHLMAWIFDNLKRPYRLKRLVQTLTQMLSLSYM
                     AVFSIPFVTPAIVRAVLTPRRIRRLLALADGIPPDQIHHSDAITREAANTLKIYRANY
                     FRSFFGRFRRDHFVDVPVQLIVNTADPFVRTYSFDDESRWVARLWRRDIKAGHWSPFS
                     HPQVLAASVADLVDVVEGRPPSRAMRRAQVGRQREPFSDTLVSITGAGSGIGRATALE
                     FARLGAEVVVSDVDEASVRDTAKDIAAQGGIAHAYRLDVSDAEAVEAFADEVCAEHGV
                     PDVVVNNAGVGQAGRFLDTPAEEWDRVLDINLGGVVNCCRAFARRLVERGSGGHIVNI
                     ASAAAFAPL"
BASE COUNT         1363 a         3104 c         3111 g         1722 t
ORIGIN      
        1 catctgtggc ccgacgccga tgagtccacc cgcagcgcga tcggcgctgt gatcgccgag
       61 cggatgaact cagttggctg cttcgcgtct cagtgactgg gccggtggag gtcgtcggtg
      121 tgccggatca tgcgctccga caattcgatg aaccggttct catggccgcc gggcacggag
      181 tagcccagcc ttcttacctc catcgcgaac ccccgcacgt cgcgggccaa ctgttccaac
      241 gcattcatgg cggacaatca ctctctctct taggcacctg gggggtataa cccggaacat
      301 aacggcggga aacatcggga ttcctcccac gtgtcgggtg ccacttccgt tactgcggac
      361 tcactgcgga ccgagagggt gtccttgcgg cattgtcgca ggtcagcgcg ttagctggct
      421 cacacgtcga agtacagcgc gaactcgtac gggtgcggcc gcacctggac cggcaggatc
      481 tcttgctccc gcttgtaggc gatccacgtc tcgatcaggt cctcggtgaa cacgccgccc
      541 tcggtgaggt attcgtggtc ctcctcgagc ttgtcgatca ccgacgccag cgacgtcggc
      601 gcttgcggga tgttggcggc ctcgtccggc ggcagctcgt agaggtcctt gtcgatcggc
      661 gccatcggct cgatcttgtt cttgatcccg tcgatgcccg ccatcagcat cgccgcgaac
      721 gcgaagtacg ggttgcccga cgagtcggga caccggaact ccaggcgctt ggccttcggg
      781 ttgttgcccg tgatcgggat gcgcacacaa gccgaccggt tgcgctggct gtacaccagg
      841 ttgatcgggg cctcgtagcc cggcaccaga cgcttgtacg agttcgtggt cgggttcgtg
      901 aacgccagca gcgacggcgc gtggtgcaag atgccgccga tgtaatggcg agcggtgtct
      961 gacagccccg cgtagccgga ctcgtcgtgg aacaacggct cgccgtcctt ccacagcgac
     1021 tggtgagcgt gcataccgga cccgttgtcg ccgaacagcg gcttaggcat gaacgtcacg
     1081 gtcttgccgt tctcccaggc ggtgttcttg atgatgtact tgaacagcag caggtcgtcg
     1141 gcggcgtgca gcatcgtgtt gaatttgtag ttgatctccg cctgtcccgc ggtgcccacc
     1201 tcgtgatggc cgcgctcgag ggtgaagccg gcgttgatca agttcgtcgt catcttgtcg
     1261 cgcaggtcga cgtagtggtc atacggcgcc accgggaagt agccgccctt ggggcggacc
     1321 ttgtagccgc ggttgggggt gccgtcaagc tcggtggcct caccggcgtt ccagtacccg
     1381 gactccgagt cgacctcgta gaacgtgctg ttgatcgacg agtcgtagcg gaccgagtcg
     1441 aagatgtaga actccgcctc ggcgccgaag tacgcggtgt cggcgatgcc ggtgctggtc
     1501 aggtagttct ccgccttgcg agcgacgttg cgcgggtcgc gtgagtacgg ctcgcgggtg
     1561 aacgggtcgt gcacgaagaa gctgatgttg agcgtcttgg cggcgcggaa cgggtcgatc
     1621 cgggcggttt cggggtcggg cagcagcatc atgtccgact cgtggatcga ctggaagccg
     1681 cgaatcgagg atccgtcgaa cgcgagcccg tcctcgaaca cgtcctcgtt gaacgccgaa
     1741 gccgggatgg agaagtgctg gacgatgccg ggaagatcgc agaaccggac gtcgacgtac
     1801 tcgacatcct cgtcagcgat cattttgaag atgtcgtcgg cggtcttttc tgccactgtt
     1861 ggaggtctcc ttactctctt atcaaacccg cggctgacgc tatggagccg atgttgcacg
     1921 cccgtcaacc gtatgttgcg ccgacgttac gcaatgtcac cgcacctatg cttgggcgcg
     1981 tggcgctcac cgattccggt tacgcagggg agagcctcgg gttgcctgcc agcggtccgc
     2041 gttcgctggc cgggttcggc cggagaatcc tcgccctgct cctcgactgg ctcatcgcct
     2101 acggcctggc cgggctggtc atgtcgttcg ggtggttgac gccgcagacc ctggccacgg
     2161 ctgtgctggt catctggttt tgcctcggtg tggtgtccgt gcgactgttc ggattcacgc
     2221 ccggtcagtt cgcgctcggg ctgatggtgg tctcggtcga cgaccggatt cacgtcggcg
     2281 caggacgcgc ggcggcgcgt ggactgctga tcgcgctggt ggtgccgccg ctgttcactg
     2341 attctgacgg gcgcgggctg caggaccgcc tcaccagcac tgccgtcgtc cggcgctagg
     2401 tggcataact tccgcgttca ctctgcgctg agggcgtgac ttactcgagg aaaaaggtca
     2461 gcgcctgcgc acggtgcgct gcatgtttcg cgtcttggcc tgcgtcggca gcggcccctt
     2521 cggcatctgc gccatgccgc tcttagttcc cagcgcggcc agccgtgact ccagcgaatc
     2581 catctgcttg gccgtgatgt tggccggcag cctggtgagg tggcgctcca gcttggccag
     2641 cggcacctgc ccgtcgccgt tgccgacgat cacgtcgtag atcgggacgt cgccgatcag
     2701 cctggcggcg cgcttcttct cttgagccag taacggtttc acccgggtgg cggatccctc
     2761 cgcgacgaaa atcacgccgg ggcgcccgat cacccggtgc accgcgtcga agtggcccgt
     2821 ggcggccact ccgggcgtca ctcgccactt gccgcgcaga ttgtccagcg cccacgccgc
     2881 cgcgccggtc tggccctcgg ccttgctgta caccgaccgc tgcgcgcgcc ggccgaagat
     2941 gatgaatgcc accagcgcgc ccagcacgac acccagcggg atcagcgtca gccccgcaac
     3001 gccgctgatg aacaggcccg cagccaccga tgccgcgacg atcagcacga acgcgccgat
     3061 catgtacgga agcagccgct tgtcctcctt gcgctggaga ttgaaggcct gccacagctg
     3121 gctgcggcgt tccttggacg ctgccttgcg ggctgtcttc gcctcggcct tcgcggcttt
     3181 gattgcggcg gaatcgcggg tcttggccat gtggcaagaa tacgggtcgt cgttacgcgc
     3241 gagtccggcc cgcgactgcc tccgcataca gccgccccgc ccgatacgac gagcgcacca
     3301 ggggcccggc gagcacgccc gcgaagccca gccccttcgc gaagtcggcc agctcgacga
     3361 actcgtcggg gtgaacccag cggttgaccg ggtggtgacg caccgacggc cgcaggtact
     3421 gggtgatcgt gacgatgtcg cagcccgcgt tcttcagctc ggtcagcgcc gtatgcacct
     3481 cgtcggtggt ctcgcccatg ccgaggatga ggttgctctt ggtcaccagg ccgtcgtcgc
     3541 gggcggcggt cagcacatcg agactgcgct ggtatccgaa cgcgggccgg atgctcttga
     3601 aaattcgggg aaccgtttca acgttgtgcg ccaacacttc tggccgcgca ccgaacactt
     3661 gctgcagcag gtcaggctcg ccgttgaagt ccgggatcag gagttccacg ccggtgttcg
     3721 ggttcagttc cttgatctgg cgcacggtct gggcgtacag ccacgcgccg ccgtcgggca
     3781 ggtcgtcgcg cgccactccc gtgacggtcg aataccgcaa tcccatcgct gcgacgctct
     3841 cggcgacgcg gcgtggctcg tcgcgatcca gttcggcggg tttgcccgtg tcgatctgac
     3901 agaaatcgca gcgacgcgtg cactgttccc cgccgatcag aaacgtcgcc tcgcggtcct
     3961 cccagcattc gtagatgttg gggcagccgg cttcctcgca gacggtgtgc aatccctcgc
     4021 ggcgcaccag gcccttcagc gcggtgtatt ccggtcccat ccgggcgcgg gtcttgatcc
     4081 acggcggctt gcgctcgatc ggggtttgcg cgttgcgcac ctcgaggcgc agcagcttgc
     4141 gcggtcccct gctcatggca tcgatgctag tgcgaggcgg ccgtcgagcg cggcgcacac
     4201 ggcgtcggcc acccggccaa ccacgtcgtc cactctcacc cgtcgaccca gttcggcggt
     4261 cagcgacgtc acccctgcgt cggcgatgcc gcacggtacg atcgcgtcga acgcatccag
     4321 gtcgcagtcg cagttcagcg cgaagccgtg cagcgtcgtc gcccgctgca cccggacccc
     4381 gatcgcgccg actttacgtt ggggagtgcc gggcagccac accccggagc ggccctcgac
     4441 gcggccgaca gaaaggccca gctccgtgca cactgcgatc aacgaatcct caaggcgccg
     4501 aacgtagttc acgacgtcga tggggtcggc cagcccgacg atcgggtagc cgacaagctg
     4561 gcccgggccg tgccaggtga tcttgccgcc gcgatcggtg tcgaccaccg gtgtgccgtc
     4621 cagcgggcgc tcatgcggtt gtgtgcgctt gccggcggta tagaccgacg ggtgctgcag
     4681 cagcaggagc gtgtccggtc caccggccac ccgggcggcc acgagctcgc gttgcagttg
     4741 ccacgcctcg tcgtagccga cagtgccgag ctgccgcacc tcgatcggcg cggcaccggc
     4801 ccggataggg cgcatacctc gacggtacta aaggacgtcg gtgcgcgaca tcgcgtacga
     4861 cagcgcttcg ccgatggtgt tgtggtggaa cgcgaaaccg gcgcgctcca gggcagccgg
     4921 tatcgcgcgc tgaccggcaa gcagcccctc gtccgcgaat tcgccgagca accgccgcag
     4981 cacgaccgcg ggcatcggca acggggtggg ccggttgagg gcacggccca gcgcggcggt
     5041 gaactccgcg ttggtgacgg gcgcgggacc ggtgaggtta agagaaccgg acacatcgtg
     5101 tgagatcgcg aacagcagcg cgcgcacctc gtcttcaagg ctgatccacg gcatgtactg
     5161 acggccgttg ccgagtcggg cgccgagtcc cagcgagaac agcgggcgaa gacggctgag
     5221 caggccaccg ccgggggaga gcaccaaacc cgtccgggcc agcaccactc gcacgcccgc
     5281 ctgctgcgcc ggtagtgtcg cggcctccca gtcctcgcac agctgcgcga ggaaacccgt
     5341 tccggcaggg gcggtttcgt cgacaacgcg gtcgccggtg ttgccgtagt agcccaccgc
     5401 gctggcgttg atcagcacct gcaccccggc ttcgacgacc gcggcggaga tcacctcggt
     5461 gggggcgatc cggctgtcgc gcagttgctg cttgtacgcg cccgaccatc gtttgtcgcc
     5521 gacgcccgcg ccgcacaggt tcaccacggc gtccacccca cgcagcgcgt cggggtcgaa
     5581 ctcgccggtc tgcgggttcc agtgcagttc gtcgctgttc gacggggcgc ggcgcacaat
     5641 gcgcaccacg cggttgtcgg aggcgcgcag cgcggtgacg agcgccgacc cgatcaggcc
     5701 cgaggacccg gcgatcacga tcagtgcatc ggccatgtgt cagagcccca agtcggcctc
     5761 gaacgctccc tcttccaagc gccgcttgat cgtcgtgagg aaccggccgg cgtcggcgcc
     5821 gtcgatcagc cggtggtcgt acgtcagcgg caagtaggac accgagcgaa tgccgatcga
     5881 ctcgttgccg agcttgtcga cgatcacccg cggccgtttg acgatggcgc cggtgcccag
     5941 catggcggcc tgcggcggca ccaggatcgg ggtgtcgaac agtgcgccct ggctgccgat
     6001 gttggtgatc gtgaacgtgc cgccggacag ctcgtcgggc ttgaggttgt tgctgcgcgc
     6061 acggtccgcg atgtcggcga tcgcacgggc aagaccggcc agcgacaggt caccggcgtg
     6121 gtggatcacc ggggacaaca ggccctgctc ggtatcgacc gcgatgccga gatgctcggc
     6181 gtcgtagtag gtgatctcct tggtttcctc gttgtagctg gcgttgacgt tcgggtggat
     6241 cttcagcgca tcgatgaccg ctctggcgat gaacggcagg tacgtcagct tgacgccttc
     6301 acgctcggcg aagtcatttt ttgctcgcgc ccgcaacgcc acgatcttgg tcatgtcgac
     6361 ctcgtgggtt tgcgtgagtt gcgctgtggc ctgcaaagat tcgcgcgttt tcttcgcggt
     6421 gatctgccgg atgcggttgg ccttctgagt ggttccgcgc aggtgggcca gcgccgaagt
     6481 cggcttctcg gcggccgccg ctgggtcctt ggccggctcc ctcttcggct cttccttcgc
     6541 cggtgccttg gcctcttcgg cagcggccag cacgtcctgc ttgcgaatcc ggccaccgac
     6601 accggtgccc ttgaccttcg cgaggtcgac gttgttctcg gaggcacgct tgcgtaccag
     6661 aggagtgacg tacggcgtgc cgtcaccatt tgatgacgcc gccgcgggag cgggttcctt
     6721 ctgtggttcg ggcttgggct cttccttggc ctccggctca ggcttcggct ccgcgggcgc
     6781 ctcgccgctt tcgcccggct ctccgatttt cgcgagctca ccgccgacct cgacggtgtc
     6841 gtcctcctcg gccgtgatgg acagcagcgt cccggacacg ggggacggga tctcggtgtc
     6901 gaccttgtcc gtggatacct ccagcagcgg ctcgtcggcc tccactttgt cgccgacctt
     6961 cttgagccag cgagtcaccg tgccttcggt gaccgactcg ccgagttcgg gcatcttcac
     7021 cgacgtcgac ttgccgtcgc tcgacgactt ggctgccggc ttggcgtcgg gctcctcggg
     7081 ctcctcggcg gcgggctcgg gttgcgcctc gggttcttcg gccggcttct cctctttttc
     7141 ttcggcggat tcctgcgcgg gctgctcgtc gccaccggac tcgccgtccg catcgattgt
     7201 ctgctcttcg cctgacgcct catcgccgat caccgccaac tcgccgccga cctccacggt
     7261 gtcgtcttcc tgtgcgacga tctttttcag gacaccggcc gccggcgacg gaatttcggt
     7321 gtcgaccttg tcggtggata cttcgagcag cggctcgtcg cgttcgaccg tgtcgccttc
     7381 ctgcttgagc caacgggtca cggtcccttc ggtcacgctc tcaccgagtg ctggcatctg
     7441 cacggagaag gccatgtctg ttgactcctc gagctcagtc ggacgggtcg acctctggct
     7501 taccacatca tctttcccca cagcgcccac tgttgaacct ctcgggggtg gcactatcga
     7561 ggacgacgca aacaggagag gcgtttcgtt gggattcttc gacaggtttc gcggcggtcg
     7621 gcgcggcgag tctgacgcgg acatcgccgc ggaccaccgc tacctccgcg actgggcgcg
     7681 tgcgcgcact ggtgtggagg cgttcgtcga accccagacg agcgtgaccg acgtgaccgt
     7741 ggtcctggtc gcaggcgacg gggagtggac gcggcgacgc gtcggcggcg cgtccggggc
     7801 gcggcgtctc ggggagcagc tgcgcatccc ggtctacgac gtccaacgcg tcggctaccc
     7861 acagcgaatg cgcgactacg acgcacgtcg ccgcgccgag cggcgtcgcg cggaggaggc
     7921 tgaatgatcg tcgacaacgg cgacgtgcgg atcgccgtct acgagcacgg caaccccgac
     7981 gggccgaccg tggtgatggt tcacggctgg cccgactccc acgtgctgtg ggacggcgtg
     8041 gtaccgctgc tggccgaccg gttccgactg atcacctacg acaaccgcgg tgtcggcccg
     8101 tcgtcgtcgc cgaagtccta tcgcgagtac acaacggcgg gcctcgccga cgacttcgcg
     8161 gccgtcatcg acgcggtcag cccgggccgg ccggtgcacg tcctcgccca cgactggggc
     8221 tcggtgtcgg tgtgggagta tctggctcgg cccgaagcgc gcacccgcgt cgcgtcgttc
     8281 acgtcggttt ccggtcccag ccaggaccat ctgatggctt ggatattcga caacctcaag
     8341 cgcccgtacc ggctgaagcg gctggtgcag acgctgactc agatgctgtc gctgagctac
     8401 atggcggtgt tctcgatacc gttcgtgaca ccggcgatcg tgcgtgccgt gctgacgccg
     8461 cgccggatcc gccgactgct cgcgctcgcc gacggcatcc cacccgacca gatccaccac
     8521 tccgacgcca tcacccgcga ggcggcgaac acgctgaaga tctaccgcgc caactacttt
     8581 cgctcgttct tcgggcgatt caggcgcgac cactttgtcg acgtcccggt gcagttgatc
     8641 gtcaacacgg cggatccgtt cgtccgcacg tacagcttcg acgacgagtc ccgttgggtg
     8701 gcacggctgt ggcgccgcga catcaaggct gggcactggt cgccgttctc gcatccgcag
     8761 gtgctggccg catcggttgc cgacctcgtc gacgtcgtcg aaggacgtcc gccgtcgcgg
     8821 gcgatgcggc gggcgcaggt cggccggcaa cgagagccgt tcagtgacac gctggtgtcg
     8881 atcaccggcg caggaagcgg catcggccgc gccaccgccc tcgaattcgc ccggctgggc
     8941 gccgaagtgg tggtcagcga cgtcgacgag gccagcgtcc gggacaccgc gaaagacatt
     9001 gcggcacagg gtggaatcgc acacgcgtac aggctggacg tgtccgacgc cgaagcggtc
     9061 gaggcgttcg ccgacgaggt gtgcgccgag cacggcgtcc ccgacgtcgt ggtgaacaac
     9121 gccggcgtcg gccaggcggg acggttcctg gacaccccgg ccgaagaatg ggaccgggtg
     9181 ctcgacatca acctgggcgg agtcgtcaac tgctgccggg cgttcgcgcg caggctcgtc
     9241 gaacgtggca gcggtgggca catcgtcaat atcgcgtccg cggctgcgtt cgcgcccctg
//
LOCUS       DASWBF010000327         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_760956,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000327 DASWBF010000000
VERSION     DASWBF010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760956"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1436
                     /locus_tag="VF874_11945"
     CDS             216..1436
                     /locus_tag="VF874_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9176745.1"
                     /translation="MLDDDDSRDLARLAVPLVGSVEETDDRGLPFRLIDPAGEPVAAV
                     SDYFRDLQAAGRSAATLRSYGLDLLRWFRFLWAIGIAWNHATRVEARDFCRWMLVAGK
                     PSRPHWRRPNEVPEGSSGAVAYAPSVRAHAETVLRGFYDYHLEAGTGPLVNPFPLDRS
                     RRGARAHAHHNPMEPHRNERSGRYRPRLADRIPRAVPDAEFNEIFARLPSHRDRALVA
                     FYVSTGARASELLSATQGGADPGRQLITVTRKGTRATQELPASSDAFVWLRLYQLEMD
                     GLIPKGKRQPLWWTSRQPVRPLTYHAAHRMFERAGQAAGSTATLHALRHTAAYRMAQD
                     PDLPLTDVQFVLGHALLTTTQIYLTPRKQDVIRRVLDHHAQQIRKVTERTAPPPAPGY
                     RPETLNVLFGTGPA"
     gene            1433..3865
                     /locus_tag="VF874_11950"
     CDS             1433..3865
                     /locus_tag="VF874_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9176746.1"
                     /translation="MTTLDIEPDVDRTPTIVRTHPRPAPRQVETDWPTTCLDRKQAWQ
                     LLNSAPLTPSRVDIRHPNAYPTGVGLLLDWLADQPGQTWQQRWIATGADADGRCWRQI
                     PAQWLREHGHPKEWLQYWLFRALLAAIAADLIRPSLSWLVTAAFRRGTLLNVVSGYRD
                     PDGFARLRALCSADSDINAAAANRTTNRAALILAAKGGTLADITPGDVLALFDVEADT
                     HATSVGATHLFYRVLHALGGFGAEAPATLRELRAAGQRSPEELIDRYELVCQPIRNLL
                     VDYLRERQPALDYTSLDALAHYLGGLFWSDLERHHPGISSLHLSAEVASAWKQRLRTI
                     TKVSRGADGKTVRTTAERVNYRKCLTPVRAFYLDLACWAAEDPARWGPWVVPCPVGAE
                     EVNQRRDKRRRKSRMDARTRERLPVLPVLVRDLDERRKTAAALLDAARRTEPGQQFSV
                     AEQTLTRIRPGHSAAAKIWVQEPQTGKRRDLGWEEDRAFWTFAAVEVLRSTGIRIEEL
                     TELSHHSLVQYRLPSTGEMVPLLQITPSKTDAERLLVVSPELAEVLSAIINRVRGATG
                     AIPLVRAYDKAERNWSPPLPLLFQRRVGFEHRAISSQAIRRMLTAAIDHTRLTDPAAG
                     EPLRFTPHDFRRLFITDAVLNGVPPHIAQVIAGHRDINVTLGYKAVYPDEAIQSHLAF
                     LARRRALRPTDEYRQPTDEEWQRFLGHFERRKVSIGTCGRAFGTPCIHEHACVRCALL
                     WPDPAQRSRLVEICDNLQARIAEAEREGWLGEVEGLQVSLAGAKDKLAQLDRRSTNTT
                     DLAIPTVTNNNS"
     gene            complement(3870..>4049)
                     /locus_tag="VF874_11955"
     CDS             complement(3870..>4049)
                     /locus_tag="VF874_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176747.1"
                     /translation="VAMVAAAVIDDGEPDTARRAGLEVYGDSAYGTGAARAAYQRDGH
                     DTVFSELDRRPGMHP"
BASE COUNT          703 a         1399 c         1294 g          653 t
ORIGIN      
        1 gaaggtcacc gtccgtttgg cggtcatggt gcgggtgtgc ccgccggggc aggtgatggt
       61 cccggcctgc tcgtcgatag tgaactcgtc gagggtaaac ccgccggcca ccgcggggac
      121 cagcggcttg ggcttgatca cctgttaact gaactgcgac acgcgtacgt gcaggttgac
      181 gggtagaaat tgattccgtt gttatcgtcg cgatcatgct cgacgacgac gattctcgag
      241 atctggcgag gttggccgtg ccgttggtcg gctcggtcga ggagactgac gaccgggggt
      301 tgccatttcg gttgatcgac ccggccggtg agccggtggc tgcggtgtcg gactactttc
      361 gtgacctgca ggccgcgggc cgatcagctg cgacgctccg ctcctatggt ctggacctgt
      421 tgcgttggtt tcggttcctg tgggcgatcg ggatcgcgtg gaaccacgcc actcgggtcg
      481 aggctcggga cttttgtcgc tggatgttgg tggccggcaa gccgtctcga ccgcattggc
      541 gccgaccgaa cgaggtgccg gaggggtcgt cgggcgcggt ggcgtatgcg ccgtcggtgc
      601 gcgcgcacgc cgagacggtg ctgcgcggct tttacgatta ccacctggag gcggggaccg
      661 ggccgctggt caacccgttc ccgctggacc ggtcgcggcg cggtgcacgg gcgcacgcgc
      721 atcacaatcc catggagccg caccgcaacg aacggagcgg acggtatcgg ccgaggcttg
      781 ccgatcggat tccgcgcgcg gtccccgatg ccgagttcaa cgaaatcttc gcccggctgc
      841 cctcgcaccg ggatcgggcg ctggtggcgt tctacgtgtc caccggcgcg cgggcctcgg
      901 agttgctgtc ggccacccag ggtggtgcgg atccgggcag gcagctgatc acagtgacac
      961 gcaaaggaac tcgcgcgacc caggaacttc ccgcctcgtc ggacgcgttc gtctggctgc
     1021 ggctctacca gctggagatg gacgggctga ttccgaaggg caaacggcag ccgttgtggt
     1081 ggacgtcgcg acaaccggtg cggccgttga cctatcacgc cgcgcaccgc atgttcgagc
     1141 gcgcggggca ggcggcgggc agcaccgcca ccctgcatgc tttgcggcac accgccgcct
     1201 accggatggc acaggatccc gacctgccgc tgacggacgt gcagttcgtt ctcggtcacg
     1261 cgctgctgac caccacccag atctacctca ccccgcgcaa gcaggacgtg atccgccgtg
     1321 tcctcgacca ccacgcgcaa cagatccgca aagtcaccga gcggacagca ccgccgccgg
     1381 cacctgggta tcggccggag acgctgaacg tgctgttcgg gaccggtccg gcgtgaccac
     1441 gctcgacatc gaacccgatg tcgaccggac gcccaccatc gtgcgcactc atccgcgtcc
     1501 ggcgccacga caggtcgaaa cagactggcc aacaacctgt ctggaccgta agcaggcatg
     1561 gcagctgctc aacagcgcac cgctgacgcc gtccagggta gacattcggc acccgaacgc
     1621 ctacccgacc ggggtgggcc tgttactgga ctggcttgcc gaccaacccg gacaaacgtg
     1681 gcagcagcgg tggatcgcca ccggtgcgga tgccgacggc cgctgttggc gccaaattcc
     1741 ggcccagtgg ctgcgtgagc acggacaccc gaaggagtgg ctacagtact ggctgtttcg
     1801 ggcgttactg gccgcgatcg cggctgatct gatccgcccg tcgctgagct ggttggtgac
     1861 cgccgccttc cgcaggggaa ctctgctcaa cgtcgtgtcg gggtaccgcg atcctgacgg
     1921 attcgcccgg ctacgagcgc tgtgttcggc cgactcggac atcaacgcgg cagcggccaa
     1981 ccgcaccacc aatcgcgccg cactgatcct ggccgcaaag ggcggcacac ttgctgacat
     2041 cacgcccggc gatgtgctcg cgctgttcga tgtcgaagcc gacacccacg ccacctcagt
     2101 cggcgccact cacctgttct accgagtcct gcacgcattg ggcggtttcg gcgccgaggc
     2161 gccggcaacg ctgcgggagc tgcgagctgc cggacagcgc agccccgagg agttgatcga
     2221 ccggtacgaa ctggtgtgcc agccgatccg aaatctgctg gtcgactatc tacgggaacg
     2281 acaaccggcg ctggactaca ccagcctcga cgcgctcgcc cactacctcg gcggcctgtt
     2341 ctggtccgat ctggagcgcc accaccccgg catctcgagc ttgcacctgt cggccgaggt
     2401 cgcctcggcg tggaagcagc ggttacgcac gatcaccaag gtgagccgtg gcgccgacgg
     2461 gaagactgtg cggaccaccg ctgagcgagt caactaccgc aagtgcctga caccggtgcg
     2521 cgcgttctac ctcgatctgg cgtgctgggc ggcggaagat ccggcccgct ggggtccctg
     2581 ggtggtgcca tgcccggtcg gagccgaaga ggtaaaccag cgaagagaca agcgccgccg
     2641 caaatctcgc atggatgccc ggactcggga acggttgccg gtactgccgg tgttggtccg
     2701 cgacctcgac gagcgccgca agacagcggc cgccctgctc gacgcggcac gacgaaccga
     2761 acccggccag cagttcagcg tcgccgagca gacgctgacc cgcatccgac caggccactc
     2821 agcagcggcc aagatctggg tgcaggaacc acagaccggc aagcgccgtg acctgggctg
     2881 ggaggaagat cgcgcgtttt ggaccttcgc cgcggtcgag gtgttgcgct ccaccggcat
     2941 ccgcatcgag gaactcaccg aactgtccca tcacagcctg gtccaatacc ggctgcccag
     3001 caccggcgag atggtgccgc tgctgcagat caccccgtcg aagaccgacg ctgaacggct
     3061 gctcgtggtc agcccggaac tggccgaagt gctgtcggcg atcattaacc gcgttcgcgg
     3121 tgcgaccggc gcgatcccgc tggtgcgtgc ctacgacaag gcggaacgaa actggtctcc
     3181 gccgctgccg ctgctgttcc agcgacgcgt aggcttcgag catcgcgcga tcagcagcca
     3241 ggccatccgc aggatgctca ccgccgcaat cgatcacacc cggctcaccg atcccgcggc
     3301 cggcgaaccg ctgcgattca ccccacacga ctttcgccgc ctgttcatca ccgacgccgt
     3361 gctcaacggc gtgccaccgc acatcgctca agtcatcgcc ggccaccgcg acatcaacgt
     3421 caccctcggc tataaggccg tctaccccga cgaggctatc caatcgcacc tggcttttct
     3481 cgcccgccgg cgcgcgctac gcccgaccga cgaataccgc caacccacag acgaggaatg
     3541 gcagcgcttc ctcggccact tcgaacgtcg caaagtttcc atcggcacct gcggacgcgc
     3601 cttcggcacc ccctgcatcc atgaacatgc ttgtgtccgt tgcgcattgc tctggccaga
     3661 ccccgctcaa cgatccagat tggtcgagat ctgcgacaac ctgcaagccc gcatcgccga
     3721 agccgaacgc gagggctggc tcggcgaggt cgaaggactc caggtcagcc tcgccggcgc
     3781 caaggacaaa ctcgctcaac tcgaccgtcg cagcaccaac accactgatc tcgcaatccc
     3841 cacggtcact aacaacaatt cctgacaccc tacggatgca tgccagggcg ccggtcgagt
     3901 tcagagaaca cggtgtcatg cccgtcgcgc tggtaggccg cacgtgcggc cccggtgcca
     3961 taggccgaat cgccatacac ctcaaggccg gcacggcgcg cggtgtcggg ttcgccatca
     4021 tcgatcaccg ccgcggcgac catcgcgac
//
LOCUS       DASWBF010000328         4325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_35557, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000328 DASWBF010000000
VERSION     DASWBF010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35557"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VF874_11960"
     CDS             <1..805
                     /locus_tag="VF874_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9176748.1"
                     /translation="RKLILRTGPPDDVHAGMELEAQTQKLAAAEGTPVPHVLIADKSF
                     LICDFIDGETIAPRILRKLDEAARARLLEQCAQALAAIHRVDPTNVELRRDDQMALWR
                     AQLHEMGDTTATFEWTLRWLVANRPPPSPPVLVHGDFRMGNLIIDDGQLAAVLDWELV
                     HVGEIYEDLAWFCIRAWRFGAPQSRGAGGLGSIEQFLSAYERASGTTVDRVGMRWWLI
                     RATLSWGIICRYQAERHLSGRSRSVELAAIGRRVCETEWDLLDLLEATA"
     gene            802..1095
                     /locus_tag="VF874_11965"
                     /pseudo
     CDS             802..1095
                     /locus_tag="VF874_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
     gene            1108..1470
                     /locus_tag="VF874_11970"
     CDS             1108..1470
                     /locus_tag="VF874_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9176749.1"
                     /translation="MADRLFSAIEAGDVAAVAAMWSDDVTVWHAGDTRPSGKARAIRV
                     IEWFVNASSDRHYDVLDRQFFPGGFVQQHVLHGTTRAGAPYSLRVGIVIRVGADALID
                     RIDEYFDPADLAPLINES"
     gene            1483..2022
                     /locus_tag="VF874_11975"
     CDS             1483..2022
                     /locus_tag="VF874_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9176750.1"
                     /translation="MSTATELLKSPETVGTWLLDHNRSSFSFKNKTMWGLMNVNGRFG
                     KFSGEGHVDAGGKVSGRIDIKGTSLATGIKRRDNHLRSADFFDVENYPDITVTVNGVD
                     GADSADELNVRADLSIRGNTVALPLRVRAEVLDDGAVRLATTTTVEREQLGVSGNMVG
                     MLGKTTTLSADAVFRRAGG"
     gene            2032..2406
                     /locus_tag="VF874_11980"
     CDS             2032..2406
                     /locus_tag="VF874_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176751.1"
                     /translation="MPHILVYSDDASVREQVKLALGKRIHPELPELTYVDVATGPMVI
                     RRVETDNIDLAILDGEAAPTGGMGIAKQLKDEIDDCPPIVVLTGRRDDAWLAKWSRAE
                     AAVPLPIDPIQLADAVVGLLRQ"
     gene            2518..2895
                     /locus_tag="VF874_11985"
     CDS             2518..2895
                     /locus_tag="VF874_11985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX9176752.1"
                     /translation="MNVYFPILVLGAIAAAFAAFSVGVAVFAGPRRYNRAKLEAYECG
                     IEPMEKSVSGPHALQGQRFPIKYYLTAMLFIIFDIEIVFLYPWAVTFDALGLFALVEM
                     LLFMATVFVAYAYVWRRGGLTWD"
     gene            2900..3466
                     /locus_tag="VF874_11990"
     CDS             2900..3466
                     /locus_tag="VF874_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX9176753.1"
                     /translation="MGIEEKLPGGILLSTVEKVAGYVRAGSLWPATFGLACCAIEMMS
                     TAGPRFDIARFGMERFSATPRQADLMIVAGRVSQKMAPVLRQVYDQMAEPKWVLAMGV
                     CASSGGMFNNYAILQGVDHVVPVDIYLPGCPPRPEMLLHAILMLHAKIRDMPLGVNRE
                     EAVREAEAAALAAANPTATTLQIGGLRR"
     gene            3463..4158
                     /locus_tag="VF874_11995"
     CDS             3463..4158
                     /locus_tag="VF874_11995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9176754.1"
                     /translation="MTEPETTHPPIPSDADVIGVRRGMFGVKGSGDTSGYGRLVREVA
                     LPGSSPRPYGAYFDEVVDALIDALGDDGFTSAVERVVVFRGELTLEVRREHLVAVART
                     LRDDPALRFELCLGVSGVHYPAEAGRELHAVYPLMSITHRRRIRVEVAAPDADPHVPS
                     LFSVYPTTDWHERETYDFFGIIFDGHPSLTRIEMPDDWVGHPQRKDYPLGGVPVEYHG
                     AEIPPPDERRAYN"
     gene            4158..>4325
                     /locus_tag="VF874_12000"
     CDS             4158..>4325
                     /locus_tag="VF874_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HEX9176755.1"
                     /translation="MSAPNPPADTVVVVGGQDWDAVIAAAAQSHAGERIVVNMGPQHP
                     STHGVLRLILEI"
BASE COUNT          704 a         1469 c         1460 g          692 t
ORIGIN      
        1 gcgcaagctg atcctgcgca ccggcccacc cgacgacgta cacgccggca tggaactcga
       61 ggcgcagacg cagaagctgg ccgcagcgga agggactccg gtgccgcacg tgctgatcgc
      121 cgacaagtcg ttcctcatat gcgacttcat cgacggggag acgatcgccc ccagaattct
      181 gcggaagctt gatgaggccg cccgcgcccg gctcctcgaa caatgcgccc aggcgctcgc
      241 cgcgatccac cgcgtcgacc ccacgaatgt cgagctgcgc cgcgacgacc agatggcgct
      301 ctggcgcgcc cagctccacg agatgggcga caccaccgcc acgttcgagt ggacgctgcg
      361 ctggctggtg gccaaccggc cgccaccgtc gccgccggtg ttggtccacg gcgacttccg
      421 catgggcaac ctgatcatcg acgacggcca gctcgccgcg gtgctcgact gggagctcgt
      481 gcacgtcggc gagatttacg aggacctggc ctggttctgc atccgtgcct ggcggttcgg
      541 cgcaccgcag agccgcggcg ccggcggatt gggcagcatc gagcaattcc tttccgccta
      601 cgagcgcgcc agcgggacta ccgtcgaccg cgtggggatg cgctggtggc tgatccgggc
      661 caccctgtcc tggggcatca tctgccgcta ccaggcggag cggcatctgt ccggccggag
      721 ccggtcggtc gagctcgccg cgatcggccg gcgggtgtgc gaaacggagt gggacctgct
      781 cgatctgctc gaggcgacgg catgaccgcc ccgtacgaca ggccgaccgc tcccgaactg
      841 gtggccgcgg tcgccgagtt tctcgatgct gacgtgcgac gcgccaccga cggccaagtg
      901 aacttccacg cccgagtggc ggccaacgtg ctgcgcatcg tcgaacgcga actcggcacc
      961 gcggggccac ccgccgacga gcaagaactg gtcgccgcga tctgcgacgg tcggttcgac
     1021 ggccgggccg acgatgtgat gaccgcgctg cgcaccctgg tctacgaccg gctcgaggtc
     1081 gcccacccgg gatatgcgct ggcgtcggtg gctgaccggc tgttctccgc gatcgaggcc
     1141 ggcgacgtcg ccgccgtcgc ggcgatgtgg tccgacgacg tgaccgtctg gcatgccggg
     1201 gacacgcgcc cgagcggcaa agcgcgcgcg atccgcgtga tcgagtggtt cgtcaacgcg
     1261 tcgtccgacc gtcactacga cgtgctcgac cggcagttct ttcccggcgg cttcgttcag
     1321 caacacgtcc tgcacgggac cacccgcgcc ggagcgccgt actcgctgcg cgtcgggatc
     1381 gtcatccgcg tcggcgccga cgcgttgatc gatcgcatcg acgagtactt cgacccggcc
     1441 gatctggcgc cgctgatcaa cgagtcctaa gaggaaggcg aaatgtcaac tgccacagaa
     1501 cttttgaaaa gccccgagac cgtcggcacg tggctgctcg accacaaccg gtcgtcgttc
     1561 tcgttcaaga acaagacgat gtgggggctg atgaacgtca acgggcggtt cggcaagttc
     1621 agcggcgagg gccacgtcga cgccggcgga aaggtgtccg gccggatcga catcaagggc
     1681 acgtcgctgg ccaccggcat caagcggcgc gacaaccacc tgcgatcggc cgacttcttc
     1741 gacgtcgaga actacccgga catcacggtc acggtcaacg gcgtcgacgg cgccgacagc
     1801 gcagacgagc tgaacgttcg cgcggacctg tccatccgcg gcaacaccgt cgcgctgccg
     1861 ttgcgagtgc gcgccgaggt tctcgacgac ggcgccgtgc ggcttgccac caccaccacg
     1921 gtcgaacgcg agcagctcgg ggtcagcggc aacatggtcg gcatgctcgg caagaccacc
     1981 acactctcgg cggacgcggt gttccgtcgc gcgggcggat aacatccgtc cgtgccccac
     2041 atcttggtct acagcgatga cgccagcgtg cgcgaacagg tcaagcttgc gctcggcaag
     2101 cggatccacc ccgagctgcc ggagctgacc tacgtcgacg tcgccaccgg cccgatggtg
     2161 atccgccggg tcgagaccga caatatcgat ctcgcgatcc tcgacgggga ggccgcccca
     2221 acgggcggga tgggcatcgc caagcaactc aaggacgaga tcgacgactg ccccccgatc
     2281 gtggtgctga ccggacggcg cgacgacgcg tggctggcga agtggtcgcg cgccgaggcc
     2341 gcggtccccc tgccgatcga cccgatccag ctggccgacg ccgtcgtggg gctgctccgc
     2401 cagtagcgct tacccctagt cagcgacggt cctgatacat gtttaggctg tgggggaggt
     2461 cactgacggg cccacagccc gggtccgtca cacgcctgcg acgcatcagg gagcgagttg
     2521 aacgtctact tcccgatctt ggttctcggc gcgatagcgg ccgcattcgc ggcgttttcg
     2581 gtgggggtcg cggtgttcgc cgggccgcgc cgctacaacc gggcgaagct cgaggcgtac
     2641 gagtgcggga tcgagccgat ggagaagtcg gtcagcggcc ctcatgcgtt gcagggccag
     2701 cggtttccga tcaagtacta cctgaccgcg atgctgttca tcatcttcga catcgaaatc
     2761 gtgttcctgt acccatgggc ggtcacgttc gacgcgctgg gattgttcgc gttggtcgag
     2821 atgcttctgt tcatggcaac ggtgttcgtc gcgtacgcct atgtctggcg gcgcggcggg
     2881 ctgacctggg attgacgaga tgggtattga agaaaagctt cccggcggga tcttgctgtc
     2941 cacggtggag aaggtcgccg gctatgtgcg ggccggatcg ctgtggccgg cgacgttcgg
     3001 gctggcgtgc tgcgcgatcg agatgatgtc gacggccggg ccgcggttcg acatcgcccg
     3061 gttcgggatg gagcggttct cggccacgcc gcggcaggcg gacctgatga tcgtcgccgg
     3121 tcgggtgagc cagaagatgg ccccggtgct gcgacaggtc tacgaccaga tggccgaacc
     3181 gaaatgggtg ctggccatgg gtgtctgcgc gtcgtcgggg ggcatgttca acaactacgc
     3241 gatcctccaa ggcgtggatc atgtggtgcc cgtcgacatc tacctgcccg gctgcccgcc
     3301 gcgcccggag atgctgctgc acgcgatcct catgctgcac gccaagattc gggacatgcc
     3361 gctgggcgtg aaccgcgaag aagccgttcg cgaggccgag gccgccgcgc tggccgcggc
     3421 caacccgacg gccaccacgc tgcagatcgg cggactgagg cgatgaccga gcccgagacc
     3481 acccatccgc ccataccttc tgatgccgat gtcatcggcg tccgccgtgg catgttcggg
     3541 gtcaaaggca gcggcgacac atccggctac ggccggctgg ttcgcgaagt cgcgctgccc
     3601 ggcagttcgc cgcgacccta cggcgcctat ttcgacgagg tcgtcgacgc cctgatcgac
     3661 gcgctcggcg acgacgggtt cacgtctgcg gtcgagcggg tcgtggtgtt ccgcggcgag
     3721 ctcaccctcg aggtgcgtcg cgagcacctg gtcgcggtcg cgcgaacgct gcgcgacgat
     3781 ccggccctgc gattcgaact gtgccttggg gtttccggtg tgcactaccc cgccgaggcc
     3841 ggccgcgaac tgcacgcggt gtatccgctg atgtcaatca cccaccggcg ccgcatccgg
     3901 gtggaggtgg ccgccccgga cgccgatcct catgtgccgt cgctgttttc ggtgtacccc
     3961 accaccgact ggcatgagcg cgaaacctac gacttcttcg gcatcatctt cgacgggcac
     4021 ccgtcactga cccgcatcga gatgcccgac gactgggtgg gccacccgca gcgcaaggac
     4081 tacccgctgg gcggggtccc cgtcgaatac cacggcgccg aaataccccc gcccgacgag
     4141 cggagggcct acaactgatg agcgcaccga accccccggc cgacaccgtg gtcgtggtcg
     4201 gcggtcaaga ctgggacgcg gtcatcgccg ccgcagccca atcacacgcg ggcgaacgca
     4261 tcgtcgtcaa catgggcccg cagcacccgt ccacccacgg ggtgttgcgg ctgatcctgg
     4321 agatc
//
LOCUS       DASWBF010000329         7330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_615801,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000329 DASWBF010000000
VERSION     DASWBF010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615801"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..344
                     /locus_tag="VF874_12005"
     CDS             72..344
                     /locus_tag="VF874_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176756.1"
                     /translation="MIHRLAILGAAALAPLAFVTLVAPGVGNAIECGWGTVFDPPSDT
                     CVAAPLPPPPPPPPPPAWNGDITPGFGVSVCVPIPVPFVPSVCAGI"
     gene            complement(868..1347)
                     /gene="bfr"
                     /locus_tag="VF874_12010"
     CDS             complement(868..1347)
                     /gene="bfr"
                     /locus_tag="VF874_12010"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX9176757.1"
                     /translation="MQGDPEVLKLLNEQLTSELTAINQYFLHSKMQENWGFTELAKRT
                     RDESFEEMHHAEWVTDRILLLDGLPNYQRLFSLRVGQSLREQFESDLAVEYEVRERLG
                     PGIVMCREKQDSTTAGLLERIMADEEKHIDWLETQLELMDKLGEPLYAAQCVSRPPS"
     gene            1486..1704
                     /locus_tag="VF874_12015"
     CDS             1486..1704
                     /locus_tag="VF874_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9176758.1"
                     /translation="MYVCLCTGATSHAVADAVARGASTCNEVARACGAGSDCGRCRRT
                     VRSIIASVTPADCAGCERNGSVGVLERR"
     gene            complement(1729..2348)
                     /locus_tag="VF874_12020"
                     /pseudo
     CDS             complement(1729..2348)
                     /locus_tag="VF874_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891160.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
     gene            2398..3675
                     /locus_tag="VF874_12025"
     CDS             2398..3675
                     /locus_tag="VF874_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9176759.1"
                     /translation="MALDWVQHAIWWQIYPLGFVGAYPANPAPAPEEHRLRRIVGWFD
                     HAVELGASGIALGPIFASRTHGYDTTDHYRIDARLGDDTDFDFVVDEAHRRGLRVMLD
                     GVFNHVGTDFGHPEWFRRGRNGFHTFEGHGELITLNHSNPDVVDYVGDVMRHWLGRGA
                     DGWRLDAAYAIPDTFWANVIPAVRDAYPDAWFVGEVIHGDYAARVRAARFDSVTQYEL
                     WKAIWSSLNDGNFHELDWALRRHNDFLATFAPLTFVGNHDVTRIASQLTDPRHVAHAV
                     VIQMTTGGVPSIYAGDEYGYRGGKEERFGGDDAVRPEFASAPGSVDHDAASVFRSHQF
                     LIGLRRRHPWLHRATIEAALLDNRRYVYVSRHGNDALLVALNIDDAALSISLSDIGFQ
                     RALVLGGDAAPGSVDVEQALVGPHGWLVLRPLP"
     gene            complement(3652..4080)
                     /locus_tag="VF874_12030"
     CDS             complement(3652..4080)
                     /locus_tag="VF874_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEX9176760.1"
                     /translation="MATLQRFVDAQAPVYDAVLAELRAGAKRSHWIWFIFPQLQGLGR
                     SPTAARFAISSLAEAQAYLDHDVLGPRLRECTRLVNAIEGSSVDEIFGWPDNLKVRSS
                     MTLFARATDDNAEFFELLDKFYGGEEDPATVERLGERPQD"
     gene            complement(4083..5681)
                     /locus_tag="VF874_12035"
     CDS             complement(4083..5681)
                     /locus_tag="VF874_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX9176761.1"
                     /translation="MNGARALITTLVDGNVDVCFANPGTSEMHFVAALDDVPEMRGVL
                     TLFEGVATGAADGYARIAGKPAAVLLHLGPGLGNGLANLHNAKRARVPMIVVVGDHAT
                     YHKQYDAPLESDIDALAHSVSGWVRRTSAAQNVGPDTLDAITAATAAPGRIATLILPA
                     DVSWDDGASPLPVEKTGRHTETPVAERTLGLVAGLLRGGEPTMLFLGGDATGEEGLTA
                     ASRIVTATGARLLCETFPTRLARGTGRPAVERLGYFAEAAAAQLDGVKQLILAGARSP
                     VSFFAYPGKPSDLVPPGCDVHLLAEPGQATAALTALADEVAPGVAATVTPLSRPPLPT
                     GALTGVSIAAAVGALLPEDAIVVDESNTAGLTLPAATAGAPRHDWLTLTGGAIGYGLP
                     AAVGAAIAAPDRPVLCLQADGSAMYTPSALWTMAREQLNVTTVIYNNGAYDILRLELQ
                     RVGAQSGPPGPRAEALLDLDPPRLDFVALAAGMGVPARRATTAEEFTAALADALGSPG
                     PQLIDAVIPSMMSRLREEHQAMMG"
     gene            5763..>7330
                     /locus_tag="VF874_12040"
     CDS             5763..>7330
                     /locus_tag="VF874_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9176762.1"
                     /translation="MLQSSDDEAVTDSQTAEQATDEQATETRTLRRRRPRRLLSRVSI
                     QSKLVVMMVLCTIVAAAVVGFIAFHAGRSSMRIQVFNRLTELRESQSRALNTEFTDLK
                     NSLIIYSHGANTVPALQAFIAGFDQLANATIDPAQQQSIVDFYNNTFLKEIEQYSNAK
                     PDLDAVLPTSNAQKYLQANYTAQRKSDDVAINMDDAHDGSIWSAANARYQDFFRQIVN
                     RFEFQDVLLLDTRGNVVYSAYKDVDLGTNILTGPYNGSKLRTAYENALASNSDDYVGI
                     TDFEIYQPAEGQPTAWMVSPVGVNGRTDGVMALQFPITKINRLMTFDKQWAESGMGNT
                     GESFLAGPDDLMRSDSRLFLENPSQFKHDVVQAGTPPDIADTAMRLGSTTLVQPISSD
                     ATRAAQRGQSGTMITTDYLGNETLQAYAPVQISDSGLHWSIVAKIDTAEAFARESAFT
                     KTMVLSTTGIIFLVCIAAMVVAQVFVRPIRKLEAGAQKISAGDYDVAIPVESRDEIGD
                     LTQAFNEMSRNLGI"
BASE COUNT         1251 a         2429 c         2404 g         1246 t
ORIGIN      
        1 ggccgaccgg gcgtaagatc ctggtctgac ctgggtcggg gaagatccga gggaggcctg
       61 cgatggtggg catgattcac cgtttggcaa tcctcggcgc ggccgcgttg gctccgctcg
      121 cattcgtgac actggttgcg ccgggggtgg gtaatgcgat cgaatgcggg tgggggaccg
      181 tgttcgatcc gccgtccgac acctgcgtgg cggcaccgct cccaccgccg ccaccgccac
      241 caccaccgcc agcgtggaac ggtgatatca cgccgggctt cggggttagc gtttgcgtgc
      301 ccattcccgt tccgttcgta ccgtcggtgt gcgccggaat ctaagccggg ccgcagctgc
      361 cagtagccag ccggtgacga gaccgcagtg accgcgtgtg gcgcgcagag tggcgagcgc
      421 acaacgtcat tgagatctct ctcggggacc aaagggggac cggtccgctt gcccggcgcg
      481 cgtggctgag cggttcattg aatgcgctcg gcccggtacc ccgttggtac ccccaacaca
      541 accgaaccga ccccgtacct accccgtacc gtaagcgtag gccagacgta ccaaaacact
      601 tggtgcagtt agtatctggg ttagctgcca gccctccgac caggcgttat cgaactcccg
      661 ttcgtaccgc cgaaacgacg acctggaatt ccgtttcggc ggaaagaaat taggtttggg
      721 acgccttggt cgcggggaat cggcagacag cggcgagctg agattggttg caatcgtgct
      781 cactgttagg cgatgagggg acgcaacccc gacgttaagt taagaagcta gcgcggccca
      841 gcatcgccgt ccgccggctc ctcagagtta gctcgggggg cgcgatacac actgggccgc
      901 atacagcggc tcgcccagct tgtccatcag ctccagctgc gtctccagcc agtcgatgtg
      961 cttttcctcg tcggccatga tcctctccag caggccggcg gtggtggagt cttgtttctc
     1021 gcggcacatc acgatccctg gtccgaggcg ctcccgtact tcgtactcga ccgcgagatc
     1081 gctttcgaac tgctcgcgca acgattgccc gacgcgaagc gagaacagcc gttggtagtt
     1141 cggcagaccg tcgagcaaga ggatgcggtc agtgacccac tcggcgtggt gcatctcctc
     1201 gaacgactcg tcacgagtcc gcttggccag ttcggtgaag ccccagtttt cctgcatctt
     1261 ggaatgcaag aagtactgat tgatcgcggt cagctcgctg gtcagttgct cattgagcag
     1321 tttgaggact tcggggtcac cttgcatggc agtcaatgta gtgcaggcgc ttcgaaacgg
     1381 cggtcatacc gggatgtcga cacgacctct cccccttgac ataggtaagc ctagcctgct
     1441 tggctgcgaa tgtgaaatcg acccggggac ggtgacaagc tcgcgatgta tgtgtgtctg
     1501 tgtaccggag cgacgagcca cgcggtggcc gacgcggtgg cacggggtgc gtcgacgtgc
     1561 aacgaagtcg ccagggcgtg cggtgcgggg tcggactgcg ggcgctgcag gcgcacggtc
     1621 cggtcgatca ttgcctcagt cactcccgcc gactgtgcgg gttgtgaacg aaacggatcg
     1681 gtaggtgtac tcgaaaggcg gtgacccgca gcgtcattaa tctcggagtc aacgggccca
     1741 cttcgggacg atgaacacgc cctctgcttc gaccgtgatg ccctcgtcgt cggcgagatg
     1801 cccgacggcg aacgtcttga ctccctcgat tcgctcgatc gtggcctctg cgtgcaatgc
     1861 gccgagtcgg gtggaacgca agaagcgaag cgtgatcgtc cccgtcagcg ccggccgggg
     1921 cgggttactc gcggcctcgc ccagcacgtg atcgaggacc agagcgcaga cgccgccgtg
     1981 gacgtggccg ggcggccctt cgtaggggcg ccgaggtgaa agtccaccca cgccttgcct
     2041 gagtcgtcgt ggtgcagcac cagtggcggc gcgatcgggt tgcgcgcgcc gatcgccgga
     2101 ttgccccacg ccatgccgtt gccgtcgtgg gtgaagcgca caccgaacgg cccgggcagc
     2161 tgattggcgc gcaatctcgc tgtggcagcg ccgatttggg cggtgacgtc ggcgacggtc
     2221 tcggcgtcga cctcggtcct gatcgtggcg tcgatcagct cgcgcaccga cttcgtcagc
     2281 ggctcgtaga tctggcgcag ccgggcgacg tcgtcggcgg tcaggtcgtg acgggtgaac
     2341 tccagcacgc cgccgatgct gccacagccg ccgcgggcat gcgtagactg ccgacccgtg
     2401 gcgctcgact gggtgcagca tgcgatctgg tggcagatct acccgctggg cttcgtcggc
     2461 gcctaccccg cgaatccggc tcccgcgcct gaggagcacc ggctgcgacg gatcgtcggg
     2521 tggttcgacc acgccgtcga actcggcgct tccggcatcg cgctcggccc gatcttcgca
     2581 tcgcgcacgc acggctacga caccaccgat cactaccgca tcgacgcccg cctcggcgac
     2641 gacaccgact tcgacttcgt cgtcgacgag gcgcaccggc gcgggttgcg cgtcatgctc
     2701 gacggcgtgt tcaaccacgt cgggacggat ttcggccacc cggaatggtt tcgccgcgga
     2761 cgcaacggct ttcacacctt cgagggccac ggcgagctga tcacactgaa ccactcgaac
     2821 cccgacgtgg tcgactatgt gggcgacgtg atgcgccact ggctgggccg cggcgccgac
     2881 ggctggcgac tggacgcggc gtatgccata ccggacacct tctgggcgaa cgtcatcccg
     2941 gccgtccgcg acgcataccc cgacgcctgg tttgtcggcg aagtgatcca cggggactac
     3001 gccgcacgcg tgcgcgccgc caggttcgac tccgttacgc aatacgagct gtggaaggcg
     3061 atctggagtt cgttgaacga cggcaacttt cacgagctcg actgggcgct gcggcggcac
     3121 aacgattttc tcgccacatt cgcgccgttg accttcgtcg gcaaccacga cgtcactcgc
     3181 atcgcgagcc agttgaccga cccgcgccat gtggcgcatg cggtggtgat ccagatgacc
     3241 accggcggtg tgccgagcat ctacgccggc gacgagtacg gctaccgggg cggcaaggaa
     3301 gaacggttcg gcggcgacga cgcggtccgc ccggaattcg cttcggcgcc cggctctgtc
     3361 gatcatgacg ccgcgtcggt gttccgctcg caccagttcc tgatcggact gcggcgacgg
     3421 catccgtggc tgcaccgggc cacgatcgag gccgcgctgc tggacaaccg gcgctacgtc
     3481 tacgtcagcc gccacgggaa cgatgcactg ttggtggcgc tgaacatcga cgacgctgct
     3541 ctgtcgattt ctctgtcgga catcgggttt cagcgcgccc tggtgctcgg tggtgacgcg
     3601 gcgcctgggt cggtcgacgt cgagcaggct ctcgtcggac cgcacggctg gctagtcctg
     3661 cggccgctcc cctagccgct cgacggtcgc ggggtcctct tcgccgccgt agaacttgtc
     3721 gagcagttcg aaaaactcgg cgttatcgtc ggtggcgcgc gcgaacagcg tcatcgacga
     3781 gcgcaccttg agattgtcgg gccagccgaa gatctcgtcg acggaggagc cctcgatcgc
     3841 gttgaccagc cgcgtgcatt cgcgcaagcg gggcccgagg acgtcgtggt ccaggtacgc
     3901 ctgcgcctcg gccagtgacg agatcgcgaa ccgcgccgcg gtcgggctgc ggcccagacc
     3961 ttgcagttga gggaagatga accagatcca gtgactgcgc ttggcgcccg cacgaagctc
     4021 cgcgaggacg gcgtcgtaca ccggcgcttg cgcgtcgacg aaccgctgca gggttgccat
     4081 gctcagccca tcatggcctg atgctcttcg cgcaagcggc tcatcatcga cgggatcacc
     4141 gcgtcgatca gctgcgggcc cgggctaccc agcgcgtcgg ccagcgcggc ggtgaactcc
     4201 tccgcggtgg tcgcgcgccg ggccggtacg cccatgccgg cggccagcgc gacgaagtcc
     4261 agccggggcg gatccaggtc gagcagcgcc tcggcgcgcg ggcccggcgg cccgctctgc
     4321 gcgccgaccc gctgcagttc aagccgcagt atgtcgtagg cgccgttgtt gtagatcacg
     4381 gtggtgacgt tgagctgttc gcgggccatg gtccacagcg ccgacggcgt gtacatcgcc
     4441 gaaccgtcgg cctgaaggca cagcaccggc cggtctggcg cggcgatcgc cgcccccacc
     4501 gctgcgggca ggccgtaccc gattgcgccg ccggtcagcg tcagccagtc gtgccggggc
     4561 gcgcccgccg ttgcggcggg cagcgtcaac cccgcggtgt tcgactcgtc gaccacgatc
     4621 gcgtcctcgg gcagcagcgc gccgaccgcc gcggcgatgc tcacccccgt caacgctccg
     4681 gtgggtagcg gtggccggct gagcggggtg acggttgccg ccacgccggg ggccacctcg
     4741 tcggccaacg ccgtcagcgc cgcggtcgcc tggccgggct cggcgaggag gtgcacgtcg
     4801 cagccgggcg gcactaggtc gctgggcttg ccggggtacg cgaagaacga caccggcgag
     4861 cgcgccccgg ccaggatcag ctgcttcacc ccgtcgagct gcgcggcggc ggcctcggcg
     4921 aagtagccga gccgctcgac cgcggggcgt cccgttcccc tggccagccg ggtcgggaac
     4981 gtctcgcaca gcaaccgcgc gccggtggcc gtgacgatgc gcgacgctgc cgtcaacccc
     5041 tcctcgccgg tcgcgtcgcc gccgaggaac agcatcgtcg gctcaccgcc ccgcaacaac
     5101 cccgcgacaa gacccaaagt ccgctccgcc accggggtct cggtgtgcct tcccgtcttc
     5161 tcgacgggaa gaggtgaggc gccgtcgtcc catgacacgt ccgcgggaag gatcagggtg
     5221 gcgatccggc ccggcgccgc ggtggcggcg gtgatcgcgt cgagggtgtc gggtccgacg
     5281 ttctgcgcgg cagaagtgcg gcgcacccag ccggacacgc tgtgggccag tgcgtcgatg
     5341 tcggactcca gcggcgcgtc gtactgcttg tggtaagtcg cgtggtcgcc gacgaccacg
     5401 atcatcggca cccgggcccg cttggcgttg tgcagattgg ccagcccgtt gcctaggccc
     5461 gggccgaggt gcagcagcac agccgcgggc ttgcccgcga tgcgggcata gccatcggcc
     5521 gcgcccgtcg ccacaccctc gaacagggtc agcacgcccc gcatctcggg cacgtcgtcc
     5581 agcgccgcga cgaaatgcat ctccgaagtc ccagggttgg cgaaacacac gtcgacattg
     5641 ccgtcgacga gcgtggtgat gagtgcgcgc gccccattca cagcttgtcc tccctttcga
     5701 cgagtcgttt tcaagctagt ctgctgcctg atcgacgcgc tgccagtgag agaaggaagc
     5761 cgatgctgca atcgtctgac gacgaagcgg tgacggattc gcagacggcg gaacaggcca
     5821 ccgacgagca ggcaaccgaa acgcgaacct tgcggcgcag acggccacgc aggttgctct
     5881 cgcgcgtttc catccagtcg aaactcgtcg tgatgatggt gctgtgcacg atcgtcgcgg
     5941 ccgctgtcgt cgggttcatc gccttccacg ccgggcgcag ttcgatgcgc attcaggtgt
     6001 tcaaccggct gactgaactg cgcgagtcgc agtcgcgggc gctcaacacc gaattcacgg
     6061 acctgaagaa ctccctgatc atctattccc acggggccaa caccgttccg gcgctccagg
     6121 cattcatcgc cggcttcgac cagctggcca acgcgacgat cgatcccgcg cagcagcagt
     6181 cgatcgtcga cttctacaac aacacattcc tcaaggagat cgagcagtac agcaacgcca
     6241 aacccgatct cgacgcggta ttgccgacca gcaacgcgca gaaatatctg caggccaact
     6301 acaccgcgca gcgtaagtcc gacgatgtcg cgatcaacat ggacgacgcg cacgacggca
     6361 gtatctggtc ggccgccaac gcccgctacc aagatttctt ccgccaaatc gtgaaccggt
     6421 tcgaattcca ggacgtgctg ctactcgaca cccggggaaa cgtcgtctac agcgcgtaca
     6481 aagacgtcga cctcggcacc aacatcctga cggggcctta caacggatcc aagctgcgca
     6541 ccgcatacga aaacgcgttg gcgtccaact ccgacgacta tgtcgggatc actgacttcg
     6601 agatctatca acccgccgag ggccagccca cagcgtggat ggtgtcgccg gtgggcgtca
     6661 atggacgaac cgatggcgtg atggcactgc agtttccgat caccaaaatc aacaggttga
     6721 tgacgttcga caagcagtgg gcggagtccg ggatgggtaa caccggtgag agtttcctcg
     6781 ccggtcccga cgatctcatg cgctccgact cacggttgtt tcttgaaaac ccaagccagt
     6841 tcaagcacga cgtcgtgcaa gcgggcacac cccccgacat cgccgatacc gcgatgcggc
     6901 tgggcagcac cacgctggta cagcccattt cgagcgacgc cacccgcgca gcgcaacgtg
     6961 gccagtccgg aaccatgatc acgacggact atctcggtaa tgaaacgttg caggcgtatg
     7021 cccctgtgca gatttcggac agcgggctgc actggtcgat cgttgccaag atcgacaccg
     7081 ccgaggcctt tgcgagggag tcggcgttca ccaaaacgat ggtgctgtcc accaccggaa
     7141 tcattttcct cgtctgcatt gcggcgatgg tcgtggccca agtgttcgta cgaccgattc
     7201 ggaagctgga ggccggcgca cagaagatca gcgcaggcga ttatgacgtc gcgataccgg
     7261 tggaatcacg cgacgagatc ggcgatttga cgcaggcctt caacgagatg agccgcaacc
     7321 tcggaatcaa
//
LOCUS       DASWBF010000330         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_615976,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000330 DASWBF010000000
VERSION     DASWBF010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615976"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF874_12045"
     CDS             <1..216
                     /locus_tag="VF874_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176763.1"
                     /translation="PATITEVLRLQLVAPAAPLLGRLAMRVQGVMLWLRGVPRVPWRP
                     MPEPAVTTAAPPVRIAVTDLRDSSVAS"
     gene            247..810
                     /locus_tag="VF874_12050"
     CDS             247..810
                     /locus_tag="VF874_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9176764.1"
                     /translation="MSRSPQVESDLDALLRRVARQDVDAFAAFYDATRARVFGMVSRV
                     VRDSGYSEETTQDVYLQVWRNAANFNPAAGSALAWLLTLAHRRAVDRVRAEQAATQRE
                     SRYGAATAEPAADVVADSAILRDEQRQVANCLGSLTDTQRECIELAYYGGLTYAQVSE
                     RLSANLATIKSRMRDALRALRNCLGVT"
     gene            807..1502
                     /locus_tag="VF874_12055"
     CDS             807..1502
                     /locus_tag="VF874_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX9176765.1"
                     /translation="MTEPSDFELLELAAPYALHAVTDDERADIDRRVARAPVAVADAF
                     HNEVRAVRETLAVVSAATAVEPPERVRDRVLAAVSPLQRKFHWRATLLAAAAALIIGL
                     GALTAGLALRPPAPQTTAEQVFAASDVHTVSRPIPGGGTATVVFSREKNAGVLVMNDV
                     PPPKPDTVYQMWLINDQGPTSAGTMDEKAVDPSTTAVLTDLENATTLAFTVEPGTGSV
                     KPTGQVVAELPLT"
     gene            complement(1495..2106)
                     /locus_tag="VF874_12060"
                     /pseudo
     CDS             complement(1495..2106)
                     /locus_tag="VF874_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768368.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
     gene            2279..>2527
                     /locus_tag="VF874_12065"
     CDS             2279..>2527
                     /locus_tag="VF874_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEX9176766.1"
                     /translation="MTLLVGTVGCSNPPKTVTKEDVANQIKQTKWDAAGNKPESASCP
                     VDLRPVVGAKVVCEMTINGQTVFVDVTVTGIDGDNVQLN"
BASE COUNT          413 a          835 c          869 g          410 t
ORIGIN      
        1 cccgcgacga tcacggaagt tctgcggctg cagctcgtcg cgcccgcggc accgttgctg
       61 ggcaggctcg cgatgcgagt gcagggcgtc atgctgtggc tacgcggggt gccgcgcgtg
      121 ccgtggcgcc cgatgccgga gccggcggtg accaccgcgg cgccgccggt aaggatcgcc
      181 gtcaccgacc tgcgggactc gagtgtggcc tcgtgaccgc gacggctggc gctaggctac
      241 gccccaatga gccgatcgcc gcaggtggaa agcgacctgg acgcgttgtt gcgccgggtc
      301 gctcgccagg acgtcgacgc gttcgccgct ttctacgacg ccacccgggc acgggtattc
      361 ggaatggtga gccgggtggt gcgtgattcg ggctacagcg aagagaccac ccaggacgtc
      421 tacctgcagg tgtggcgaaa cgccgccaac ttcaacccgg cggcgggatc ggcgctggcg
      481 tggttgctga cgctcgccca ccgccgggcc gtcgaccgcg tgcgcgccga gcaggccgcc
      541 acccaacgcg agtcccgata cggcgcggcg acggcagaac cggcggcgga tgtggtcgcc
      601 gactcggcca tcctgcgtga cgaacaacgc caagtggcga actgcctcgg ttcgctgacc
      661 gacacccagc gcgagtgcat cgaactcgcc tactacggcg ggctcaccta tgcccaggtg
      721 tccgaacggc tctcggccaa tctggcgacc atcaagtcgc ggatgcgcga tgcgcttcgt
      781 gcgctgcgga actgcttggg cgtgacatga ccgagccatc cgatttcgag ctgctcgaac
      841 tggcggcccc gtacgcgctg cacgccgtca ccgacgacga gcgcgccgac atcgaccgtc
      901 gggtggcccg ggcgccggtc gcggtggccg acgcgttcca caacgaggtg cgcgcggtcc
      961 gcgagacgct ggccgtggtg tccgccgcga ccgcggtgga gccacctgag cgggtgcgcg
     1021 accgggtgct ggcggccgtc tccccgctgc aaaggaaatt ccattggcgc gcaacacttt
     1081 tggcggccgc tgcggcgttg atcatcgggc tcggcgcgct gacggccggc ttggcgcttc
     1141 ggccgccggc gccgcagacc actgcagagc aggtgttcgc cgcctccgac gtgcacacag
     1201 tgtcgcgccc gattccgggc ggcggcaccg cgaccgtcgt gttctcccgt gagaagaacg
     1261 ccggcgtgtt ggtcatgaac gacgtgccgc cgccgaagcc ggacacggtg taccagatgt
     1321 ggttgatcaa cgaccaagga ccgacatcgg cgggcacgat ggacgagaag gcggtggacc
     1381 cgtcgacaac tgccgtgctg accgacctcg aaaacgccac cacactggcg ttcacggtcg
     1441 agcccggcac cggctcggtc aagccgaccg gtcaggtggt cgccgagttg ccgctcacct
     1501 gagcgtcgcc gccgcagcct cggtgatgac gtcggcggcg tctttccgct gccgccacgc
     1561 acggacctgc cgcatggcgc cgttgccgtc gcggaccagc cggtgcagct catcggacac
     1621 cggcgcgtat tcacccaatg cctccaacgc tgggcggacc tgcactcggc cagcgcgccc
     1681 acaggatgct gcgccaactg gcgtcccgtc tcggcgttgc gatagatcgc ggagttggcg
     1741 gtcaacgcca aaagcagtgg tagccatggt cgcaaccagt tgctgacctg gaccgccgcc
     1801 tcctggtttg gtaccgcgac gtgcacatgg cagccgcaga tccttgctcg tgggcgatca
     1861 tgccgaagtt ctcgccgatc ttgtggtacc gcggcgggtc ggtgatcggg aagtggtgcg
     1921 gcaccgaggg cggagcgcca ccgcgagcag ccgcgcgccg cacgctgatg ccgcatccgc
     1981 tgcgacacgg cgcagccttg tcagctccgc gcggagtccg tcactggtgg cgacgaccgg
     2041 ttcgccggtg tcgttgtcga cgaggaggaa ctcctcttcg actccaatcg tcggatgctc
     2101 gtccatggtg cgtgccattc ttccaccgat gctgggaagt cactggagcc gaggcgattt
     2161 caccgcatta tgcgccggaa tgtggcgctg acgtgtcata gtgacttcac gcgacatcgg
     2221 atacgggagg gtcggggtgg ccaaatctgt tgggtcagca aacattatct gttgcgcgtt
     2281 gacgttgctg gtcggaacag ttggttgttc gaaccccccg aaaactgtca ccaaagaaga
     2341 cgtcgccaac cagatcaagc agacgaaatg ggacgcggcg ggcaacaagc cggaatccgc
     2401 gtcgtgtccg gtcgatctca ggccggtcgt gggggcgaaa gtggtgtgcg agatgacgat
     2461 caacggccag acggttttcg tcgacgtcac cgtcacgggc attgatggcg acaacgtaca
     2521 actcaat
//
LOCUS       DASWBF010000331         9585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_290044,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000331 DASWBF010000000
VERSION     DASWBF010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290044"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VF874_12070"
     CDS             complement(<1..1297)
                     /locus_tag="VF874_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9176767.1"
                     /translation="MNALPARRHFFRGDDGYEQARRSMVWNQRVPDRYPDVIVVARDG
                     DDVVAAVAYAKANGRQVGIRSGGHSWGANHLRDGGVLLDVHGLDHCTVDADRNVAVVG
                     PGKGGSVLAGELDAQGLFFPAGHCKGVCIGGYLLQGGYGWNSRVFGPACESVIGLDVV
                     TADGEQLYCDADNHPDLYWAARGAGPGFFAAVTAFHLKLYAKPAVCASSLYVYPIDCT
                     DEIFIWARATSAEVDRRVELQIVASRSVPNLGIDHPAIVFASPAFADSEQEAAKALAL
                     LGTCPAIDKAIVKIPYAPTDLLTWYDGVMSNYLADHRYSADNMWTSASAEELLPGIRR
                     IIETIPPHPSHFLWLNWGPSPSRQDMAYSLEDEIYLACYGGWMGAADDDKYADWARSN
                     MAAMADLASGIQLADENLGERPARFATDEAMARLDHIRAT"
     gene            complement(1373..1882)
                     /locus_tag="VF874_12075"
     CDS             complement(1373..1882)
                     /locus_tag="VF874_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6790 family protein"
                     /protein_id="HEX9176768.1"
                     /translation="MGSVIAVVIALIGGFVQSRRHKDRLAVDIHMVWWMVWVVGIASI
                     VGAGYHVFDGPQTAELIGYTRGDGGFQWENAMGDLAIGVVGVMAYWFRGHFWLATIVV
                     LTVQYVGDAAGHIYFWVVENNTKPYNIGIPLWTDILLPIVMWALYVWSWRRNGDAVPK
                     AASRAAKST"
     gene            complement(1894..2550)
                     /locus_tag="VF874_12080"
     CDS             complement(1894..2550)
                     /locus_tag="VF874_12080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX9176769.1"
                     /translation="MSEPNAVDVDALFDRLLIGDDPALAATRAATAAAGMPPIEVSAQ
                     HGKFLYLLASVMRARRILEIGTLGGFSTIWLARAVGPEGHVVTLEYEPKHAQVARTNI
                     DRAGIGERVDVIVGAALDTLPQLRSESFDLVFVDADKENNSAYVQWAVELSAPGSIIV
                     VDNVVRMGRVLAPAADDHQAQGVVDMLDMIAAHPRLEAAAIQTVGVKGWDGFLLARVT
                     "
     gene            complement(2561..3058)
                     /locus_tag="VF874_12085"
     CDS             complement(2561..3058)
                     /locus_tag="VF874_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04338 family metallohydrolase"
                     /protein_id="HEX9176770.1"
                     /translation="MSSRDTQRGKVYAAEQFVRTLFDRAAEHGSRAVDFFGTALTLPP
                     EGRFASVESVRAYVDSVLNHSGVQQRWPEVGPVAVRPRQGVTAAHYERRDGDAVIAVP
                     ARHDRWALRELVVLHEIAHHFCDATPAHGPEFVATFCELAGLVMGAEIEHVLRVVYSK
                     EGVRG"
     gene            complement(3088..3837)
                     /locus_tag="VF874_12090"
     CDS             complement(3088..3837)
                     /locus_tag="VF874_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HEX9176771.1"
                     /translation="MTDDKMLARIAALLRQAEGTDNAHEAEAFMAAAQRLATATSIDL
                     AVARSHAATRTSAQAPVQCTITIGNAGTRGLRTYVQLFAVIAAANDVKVDVASNSTFV
                     YAYGFPEDIDASHALYASLVMQMVRSSETYLASGAHRPTPTITARLNFQLAFGARIGQ
                     RLVQAREEATAEVKSRSASHPGTALALRNKEIELTDFYTRESKARGTWQAHRASAGYS
                     SAARRAGDRAGRRARLGSSPELPNGRTPLER"
     gene            complement(3882..4721)
                     /locus_tag="VF874_12095"
     CDS             complement(3882..4721)
                     /locus_tag="VF874_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9176772.1"
                     /translation="MATTRTERSFDGADGVRIVYDVWTPDETPRGVVLISHGYAEHAR
                     RYDHVAQRFGEAGLITYALDHRGHGRSGGKRVWLKRLSHYTDDFARLVDIAAADYPDL
                     NRVVVGHSMGGGIVFAYAADHPGDYTAVVLSGPAVAAQLEVPKPLGVAAKLLGSLFPA
                     AKVRTLDADAVSRDPEVVAKYKADPLVYHGKFPAGISKALLEVGQKQPQLAAKLTAPL
                     LIVHGEEDRLISVEGSRMLVENVASTQVVLKIYPELYHEVFNEPERHRVLDDVTSWIE
                     THL"
     gene            4669..5707
                     /locus_tag="VF874_12100"
                     /pseudo
     CDS             4669..5707
                     /locus_tag="VF874_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214696.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(5801..6727)
                     /locus_tag="VF874_12105"
     CDS             complement(5801..6727)
                     /locus_tag="VF874_12105"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEX9176773.1"
                     /translation="MARRGKVSIVGMGTVGTAIAYACLIRGSARELALYDVNAKKVRA
                     EVLDLNHGSPFVPPCRVTGGDDLAVTAESTIVIVTAGAKQHPGQSRLDLASTNVAMAR
                     KLIPQLFEQSPHAVVVMVTNPVDVVTCAAVNAVDAPRGRVFGSGTVLDSSRFRHLIAA
                     RAGIAVENVHGFIVGEHGDSEIPVWSAVTVGGVPAAEVFDQVAQEQISSEVVNAAYEI
                     IAGKGATNLAIGLSTARIIEAIMGDQHRVLPVSTVQSGAYDIDGVALSVPTVVSASGA
                     GRVLQVPLSDDELAGLHRSARTLTEAGRSLGL"
     gene            6849..7973
                     /locus_tag="VF874_12110"
     CDS             6849..7973
                     /locus_tag="VF874_12110"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HEX9176774.1"
                     /translation="MKTKGALLWELNSPFKVDEIDVGDPVAGEVQIRLHAAGMCHSDY
                     HLVNGNTPIALPALGGHEGAGVVTKVGKGVTGLEEGDHVILAFIPSCGRCQPCLKGFR
                     SLCDRGAVLLGGKAIADGTSRVHAGSTEVSPMNLLGTFAPYMTVHQDSVVKIDDSVPS
                     ETAAIMGCAVPTGFGSATNVAEVQPGETVVIVGVGGIGMSALQGAVIAGARNVVAIDP
                     VEFKREQAIKFGATHAYESMAAAIEPVINLTQGFMAEKTIIAVGDMRGEYIEEAMTLT
                     AKTGTAVVTGMGAMLGVDVKLNLFLFTMLQKTLKGNIFGGSSSHVETPRLVGLYKSGL
                     LKVDEMVTSTYRLEDINEGYQDMLDGKNIRGVIRYDEADW"
     gene            7993..8700
                     /locus_tag="VF874_12115"
     CDS             7993..8700
                     /locus_tag="VF874_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9176775.1"
                     /translation="MSPDIRRAVDRAVTTTSWLDSKHSFSFGDYYDPHNTHHGLLLVN
                     NDDVVAPAAGFDTHPHRDMEIVTWVLSGSLTHEDSAGNAGVIYPGLAQRMSAGSGIMH
                     SEKNETSDEPVHFVQMWVVPDEPGVTPGYQQQVVDIAGGLVPIASGRADDAAVAIHNR
                     YATLHGARLSPGEAVELPHTPYLHLFVARGDVTLEGAGELGEGDAVRFTASGGQRVTA
                     VAPAEILVWEMHAGLAA"
     gene            8681..>9585
                     /locus_tag="VF874_12120"
     CDS             8681..>9585
                     /locus_tag="VF874_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HEX9176776.1"
                     /translation="MRAWRRNAALVTAAALITVACGAPTTAPPKTPAPPAQAPAGLVP
                     VTVQVAPALADSPFDVARQALVPPGWTMSLWARVPKARLAAWAPDGALLVSVPADGKV
                     LKLTPGAPLRQEVLLEDLDAPHGLAFAGSTLYVAESDQVDAYTYTDGLATDRRVVADG
                     LPDDRSADLGGAYAHALKSVAVGPDGAVYFSIGSTGNVSAEDRSATPERATIMRIPPQ
                     GGPYQVFARGVRNGTGLAIAPDGSVWTAVNNRDNIAYPDPGPSYGRVIPDYVDDHPPE
                     SLARLTSGRDLGWPYCNPAGAGGPVP"
BASE COUNT         1543 a         3317 c         3199 g         1526 t
ORIGIN      
        1 aggtggcgcg gatgtggtcg agccgggcca tcgcctcgtc ggtggcgaac ctcgccggac
       61 gctcgcccag gttctcgtcc gcgagttgga tgccgctggc caggtcggcc atggccgcca
      121 tgttcgaccg cgcccagtcg gcgtacttgt cgtcgtcggc ggcgcccatc cagccgccgt
      181 agcacgccag gtagatctcg tcttcgaggc tgtaggccat gtcctggcgc gacggcgacg
      241 ggccccagtt cagccacagg aagtgtgacg ggtgcggtgg aatggtctcg atgatgcggc
      301 gaatacccgg caacagctcc tcggcggacg ccgacgtcca catgttgtcg gcggagtatc
      361 ggtggtcggc cagatagttg ctcatcacgc cgtcatacca ggtcaacaaa tcggttgggg
      421 cataggggat tttgacgatc gccttatcga tggccgggca ggtgccgagt aacgccagcg
      481 ccttcgcggc ttcctgctcg gagtccgcga atgctggtga cgcgaaaacg attgcgggat
      541 ggtcgatccc gaggttcggc acgctgcgtg acgcgacgat ctgcagctcc acccggcgat
      601 ccacctcggc gctggtcgcc cgggcccaga tgaagatctc gtcggtgcaa tcaatcggat
      661 agacgtacag gctgctggcg cagacggccg gtttggcgta cagcttgagg tgaaacgcgg
      721 tgacggccgc gaagaagccc ggccccgccc cgcgcgccgc ccagtacagg tccgggtgat
      781 tgtcggcgtc gcagtagagt tgctcaccgt ccgcggtcac gacgtcgagg ccgatcacgc
      841 tctcgcaggc cggcccgaaa accctgctgt tccaaccgta tccgccctgc agcagatatc
      901 cgccgatgca gacccccttg cagtggccgg cgggaaagaa gaggccctgc gcgtcgagtt
      961 cgcccgcaag cacgctgccg cccttgccgg ggcccaccac cgcgacgttg cggtcggcgt
     1021 cgacggtgca gtggtcgaga ccgtgcacat cgagcagcac accgccgtcg cgcagatgat
     1081 tggcccccca gctgtgccca cccgaccgga tgcccacctg gcgaccgttg gctttcgcgt
     1141 acgcgaccgc tgcgaccacg tcatcgccgt cgcgggcgac cacgatcacg tccggatagc
     1201 gatccggcac gcgctggttc cacaccatcg accggcgggc ttgctcgtac ccgtcgtcgc
     1261 cccggaagaa atgtcgcctc gccggtaacg cgttcattca caactccgtc ggctcatcgt
     1321 tttctgcgcc gagatcgaca caggggctgt ggttgggcgg aagacacgac cctcacgtcg
     1381 atttcgccgc acgggaggct gctttgggca ccgcgtcacc gttgcggcgc cacgaccaca
     1441 cgtagagcgc ccacatcacg atcggcaaca ggatgtcggt ccacagcggg atcccgatgt
     1501 tgtacggctt ggtgttgttc tcaaccaccc agaagtagat gtgcccggcg gcgtcgccga
     1561 cgtactgcac ggtcaacacg acgatcgtgg ccagccagaa gtggccgcgg aaccagtacg
     1621 ccatcacgcc gacgacgccg attgccaagt cgcccatcgc gttctcccac tggaacccgc
     1681 cgtcgccgcg ggtgtagccg atcagctccg cggtttgcgg gccgtcgaac acgtgatacc
     1741 ccgcgccgac gatcgaggcg atgccgacga cccagaccat ccaccacacc atgtggatgt
     1801 ccactgccag acggtctttg tgccgccggc tctggacaaa gccgccgatc agcgcgatga
     1861 cgacggcgat gacactgccc acttgttgaa ctcctacgtc actcgggcca gcaggaagcc
     1921 gtcccacccc ttgacaccga cggtttgaat cgcggccgcc tccagccgcg gatgcgcggc
     1981 gatcatgtcg agcatatcga ccacgccctg cgcctgatgg tcgtcggcgg ccggggcaag
     2041 cactcggccc atccgaacca cgttgtccac cacgatgatt gagcccggtg cgctcaactc
     2101 gaccgcccac tggacgtagg ccgagttatt ctccttgtcc gcgtcgacga acaccaggtc
     2161 gaacgactca gaccgcaact gcggcagcgt gtccagcgcc gcgccgacga tcacgtcgac
     2221 gcgctcgccg atgccggctc ggtcgatgtt ggtccgcgcc acctgcgcgt gcttgggctc
     2281 gtactccagc gtcacgacat gcccctcagg gccgacggcg cgcgccagcc agatcgtgct
     2341 gaagcccccg agcgtgccga tttccaagat gcggcgtgcc ctcatcaccg aggccagcag
     2401 gtacaggaac ttgccgtgct gtgccgacac ctcgatcggc ggcatgcccg cggccgcggt
     2461 ggccgcccgt gtcgcggcga gcgcggggtc gtcgccgatc agcagccggt cgaacaatgc
     2521 atcgacgtcc accgcattcg gttcgctcac ccggcccacg ctagccgcgc actccttcct
     2581 tcgaatagac cacccgcagc acgtgttcga tctctgcgcc catcaccagg ccggccagtt
     2641 cgcagaacgt ggcaacgaac tcagggccgt gagcaggcgt cgcgtcgcag aagtggtgcg
     2701 cgatctcgtg caacaccacg agttcccgca gcgcccagcg gtcgtggcgg gcaggcaccg
     2761 cgatcaccgc gtcgccgtcg cggcgctcgt agtgcgcggc cgtaacccct tgccgaggtc
     2821 gcaccgcgac cggcccgact tccggccaac gctgttgcac gcctgaatga ttcagcacgg
     2881 agtcgacgta ggcacgcacc gattccaccg acgcgaaccg tccttccggc ggcagcgtca
     2941 gcgccgtgcc gaagaagtcg accgcccgtg acccgtgctc agcggcccgg tcgaacaacg
     3001 tgcgcacgaa ctgctcggcc gcatacacct tgccgcgctg cgtgtcgcgg ctgctcaacg
     3061 ccctttgctc ttcgcgcaag cggctgctca cctctcgagc ggggtgcggc cgttgggcaa
     3121 ctcggggctg ctcccgagcc gggcgcgacg gccggccctg tcaccggctc gccgcgccgc
     3181 cgacgagtag cccgccgacg cccggtgggc ctgccacgtg ccacgcgcct tcgactccct
     3241 ggtgtagaag tccgtcagct cgatttcctt gttgcgcaac gcaagcgcag ttccgggatg
     3301 cgacgccgac ctgctcttca cctcggccgt cgcctcctcg cgcgcctgca ccagtcgctg
     3361 gccgatgcgc gcgccgaagg cgagctggaa gttcagccgc gcggtgatgg tcggcgtcgg
     3421 ccggtgcgca cccgaggcga ggtacgtttc ggacgatcgg accatctgca tcaccaggct
     3481 ggcgtacaac gcgtggctgg cgtcgatgtc ctcggggaat ccgtaggcgt acacgaacgt
     3541 cgagttcgac gcgacgtcga ctttcacgtc gttggccgcg gcgatcaccg cgaacagctg
     3601 cacgtaggtg cgcagacccc tggtgccggc gttgccgatc gtgatcgtgc actggacggg
     3661 ggcttgcgcc gaggtgcgag tagccgcgtg ggagcgcgcg actgccaggt cgatggaggt
     3721 ggcggtggcc agccgttgcg ccgcggccat gaaggcctcg gcctcgtgcg cgttgtcggt
     3781 gccctcggcc tggcgcagca gggcggcgat gcgcgccaac atcttgtcgt cggtcatagc
     3841 ggcaatctaa gggagacccc cgacgacttt gtgccgcagt ttcacaaatg cgtctcgatc
     3901 cacgacgtga cgtcgtcgag cacgcggtgc ctttcgggtt cgttgaagac ctcgtggtac
     3961 agctcgggat agatcttcag caccacctgg gtcgacgcga cgttctcgac gagcatccgg
     4021 ctgccttcga cgctgatgag ccgatcttct tcgccgtgga cgatgagcag cggcgcggtc
     4081 agcttggcgg ccaactgcgg ttgcttctgg cccacctcca gcagtgcctt ggagatgccg
     4141 gccgggaact tgccgtgata gaccagcgga tctgccttgt acttcgccac cacctcaggg
     4201 tcgcgggaca ccgcgtcggc gtcgagagtg cgcaccttcg cggcggggaa cagcgacccg
     4261 aggagtttgg ccgccacccc cagcggcttg ggcacctcga gttgagcggc gacggccggc
     4321 ccggacagca cgacggccgt gtagtcgccg ggatggtctg cggcgtacgc gaagacgatt
     4381 ccaccgccca tgctgtggcc gaccaccacg cggttcaggt cggggtagtc ggcagcggcg
     4441 atgtcgacga gcctggcgaa gtcgtcggtg taatgcgaca gccgcttcag ccacacccgc
     4501 ttgccgcccg accggccgtg cccgcggtgg tcgagtgcgt aagtgatcag accagcttcg
     4561 ccgaaccgct gggcgacgtg gtcgtagcgt cgggcgtgtt cggcgtatcc gtgcgagatc
     4621 agcacgactc cgcggggtgt ctcgtcgggc gtccagacgt cgtagacgat ccgcaccccg
     4681 tcggcgccgt cgaacgagcg ttccgtgcgg gtagtggcca tcagcagagc gtaatagcgc
     4741 gcgtaggctc gctgacgtga gcgtccttgc ccgcgaatct gacgacgatt tcctggtcca
     4801 ggcggagcca catcggcgag aactcctcgc gcattgctac cgcatgacgg gctcgctgca
     4861 cgacgccgag gatctcgtgc aggagacgta tctgcgggcg tggaaagcgt attcgggctt
     4921 ccagggcaag tcgtcgctgc ggacgtggct gtatcggatc gcgacaaaca cgtgcctgac
     4981 gtcgttggag ggcaggaaac ggcggccgct gcccagtggg ctgggcgcgc cgagctccga
     5041 ccccggcgac gagctcgtgg agcgcggtga ggtgccgtgg ctcgagccgc tgcccgacga
     5101 tccgtcggcg atcgtcgaga gccgtgagtc ggtacggctg gcgttcaccg ccgcgctgca
     5161 gcacctgtcg ccccggcagc gggcggtgct ggtgttgcgc gacgtgctgc agtggaaggc
     5221 cgccgaggtg gccgaggcga tcggcgcgtc gacggccgcg gtgaacagtc tgttgcagcg
     5281 ggcgcgggcg cagctcgagg ccgtcggccc cgacgactca ctgtccgcgc cggactcgcc
     5341 cgagacgcag gatcgcttgg cgcgctacat cgccgcgttc gagcattacg acatcgaccg
     5401 cctggtgaaa atgtttaccg ccgaggccat ttgggagatg ccgccgttcg acagctggta
     5461 tcagggcccg caggccatcg gctcgctgat ccacctgcag tgccccgcgc agggagccgg
     5521 cgacatgcgg ctcgtcccgg tcgtggccaa cggccagccc gccgccgcga tgtacatgcg
     5581 cgagggagcc aagcacgtgc cgttccagct gcacgtcgtg gacttcacgg ccgacggcgt
     5641 ggctcacgtt gttgcgttcc tcgacaccac gttgttcgcg aaattcggcc tgcctgacca
     5701 cctttagccg tcgacagcac gccggccgag agtgacgttt tgcaggcccg ctctcgcact
     5761 ttgtctgcaa aacgtgcttc ccgcgagagt tgtcgggggc ttacagcccc agcgagcggc
     5821 cggcctccgt caacgtccgc gccgaccggt gcagcccggc cagctcgtca tcagagagcg
     5881 gcacctgcag tacccggccg gccccggacg ccgacaccac agtcggcacc gacaacgcga
     5941 ccccgtcgat gtcgtaggcg ccggactgca cggtcgacac cggcagcacc cggtgctggt
     6001 cgcccatgat ggcctcgatg atcctggccg tggaaagtcc gatcgccagg ttggtggcgc
     6061 ccttgccggc gatgatctcg tacgcggcgt tgaccacctc cgacgaaatc tgctcttggg
     6121 caacctgatc gaacacctcg gccgctggca cgccgcctac cgtgaccgct gaccagaccg
     6181 ggatttcgga gtcgccgtgc tcgccgacga tgaagccgtg cacgttctcc accgcgatgc
     6241 cggcacgcgc cgcgatcagg tgccggaagc ggctggaatc cagcacggtt ccggacccga
     6301 acacccggcc ccgcggcgcg tcgaccgcgt tcacggcggc gcaggtgacg acgtcgaccg
     6361 ggttggtcac catgaccacg acggcgtgcg gggactgctc gaacagctgc gggatcagct
     6421 ttcgggccat cgcgacgttc gtcgacgcca ggtccagtcg gctctggccc gggtgctgct
     6481 tggcgcccgc cgtgacgatg acgatcgtcg actcagcggt gaccgcgagg tcgtcgccac
     6541 cggtgacccg gcacggcgga acgaacgggc tgccgtggtt gaggtcgagc acctcggcgc
     6601 gcaccttctt cgcgttgacg tcgtacagcg cgagctcgcg ggccgacccg cggatcagac
     6661 aggcgtaggc gatagctgtg cccacggtgc ccatcccgac aatcgacacc ttcccgcgtc
     6721 tggccatggc acccatactg ccaaggcgta ggtgacgacg atctctagcg ccgcttatcc
     6781 gcagccagag gtcatttcgg atggccaaat tggaacctgt tatcgagcgc tggttatgat
     6841 cgccggctat gaagaccaag ggcgctcttc tctgggagct caactcaccg ttcaaggtcg
     6901 acgagatcga cgtcggcgac ccggtcgccg gcgaagtgca gatccgcctg cacgcagcgg
     6961 gcatgtgcca ctccgactac cacctggtca acggcaacac cccgatcgcg ctgcccgcgc
     7021 tcggcggtca cgagggtgcc ggcgtcgtca ccaaggtcgg caagggcgtc accggcctcg
     7081 aggaaggcga ccacgtcatc ctggcgttca tcccgagctg cgggcgctgc cagccgtgcc
     7141 tgaagggttt ccggtcgctg tgcgaccgcg gcgccgtgct gctcggcggc aaggccatcg
     7201 ccgacggcac cagccgcgtg cacgcgggca gcaccgaagt ctcgccgatg aacctgctcg
     7261 gcaccttcgc gccgtacatg accgtgcacc aggactccgt cgtcaagatc gacgacagcg
     7321 tgccgtccga gaccgccgcg attatgggct gcgcggtgcc caccggattc ggctcggcca
     7381 ccaacgtcgc cgaggtgcag cccggcgaga cggtggtgat cgtcggcgtc ggcggtatcg
     7441 ggatgagcgc gctgcagggt gcggtgatcg ccggcgcgcg caacgtcgtc gcgatcgacc
     7501 cggtcgagtt caaacgcgag caagccatca agttcggcgc cacccacgcc tacgagtcga
     7561 tggccgccgc aatcgagccc gtcatcaacc tgacccaggg gttcatggcg gagaagacga
     7621 tcatcgccgt cggcgacatg cgcggcgagt acatcgaaga agcgatgacg ctcaccgcca
     7681 agaccggcac cgccgtggtg acgggcatgg gcgcaatgct cggcgtcgac gtcaagctta
     7741 acctgttcct gttcacgatg ttgcagaaaa cgttgaaggg caacatcttc ggcggcagca
     7801 gcagccatgt cgagacacct cggctggtcg ggctctacaa gtcgggactg ctcaaggtcg
     7861 acgagatggt caccagcacc taccgtctgg aagacatcaa cgagggctat caggacatgc
     7921 tcgacggcaa gaacatccgc ggcgtcatcc gctacgacga ggcggactgg tagcgcgact
     7981 accctgggcc gcatgagccc agacatccgc cgcgcggtcg accgcgccgt caccacgacg
     8041 tcatggctgg actccaagca ttcgttctcc ttcggcgact actacgaccc gcacaacacg
     8101 catcacgggc tgctgctggt caacaacgac gacgtcgtcg cacctgcagc gggcttcgac
     8161 acgcacccgc accgggacat ggagatcgtg acctgggtgc tcagcggatc gctcacgcac
     8221 gaagactccg cgggcaatgc gggcgtgatc tatccaggtc tcgcacaacg gatgtcggcg
     8281 ggcagcggca tcatgcactc ggagaagaac gaaacatccg acgagccagt gcatttcgtt
     8341 cagatgtggg tggttcccga cgagcccggc gtcacgcccg gataccagca gcaggtggtc
     8401 gacatcgccg gcgggttggt gcccatcgcc tcgggccggg ccgacgatgc cgctgtcgcc
     8461 atccacaacc gctacgccac gctgcacggc gcccgcctga gcccgggcga ggccgtcgag
     8521 ctgccgcaca cgccctacct ccacctgttc gtcgcgcgcg gcgacgtgac gctggaaggc
     8581 gcgggcgagc tcggcgaggg agacgcggtg cggttcaccg cgtcgggcgg ccagcgggtc
     8641 accgccgttg cacccgcgga gatcctggtc tgggaaatgc atgcgggcct ggcggcgtaa
     8701 cgcggccctg gtcaccgcgg ccgcgctgat cacggtcgct tgcggcgcac cgacgaccgc
     8761 tccgcccaag acgccggcgc cgcccgcgca ggcgcccgcc ggactggttc cggtgaccgt
     8821 gcaggtcgcg ccggcgctgg ccgattcgcc gttcgacgtc gcccggcagg cgctggtgcc
     8881 gcccgggtgg acgatgagtc tctgggcgcg ggtcccgaag gcccggctgg cggcgtgggc
     8941 gcccgacggt gcgctgctgg tgtccgtgcc ggccgacggc aaggtgctga agctgacgcc
     9001 cggcgcgccc ctgcgccagg aggtgctgct cgaggatctc gacgcgccgc acggactggc
     9061 gttcgcggga tcgacgctgt acgtcgcgga aagcgaccag gtcgacgcat acacgtacac
     9121 cgacgggctc gcgaccgacc gacgggttgt cgccgacggc ctgcccgacg accggagtgc
     9181 cgacctcggc ggcgcctacg cgcacgcgct gaagagcgtc gcggtcggac ccgacggcgc
     9241 ggtgtacttc tcgatcggct ccaccggcaa cgtctccgcc gaagaccgct ccgcgacgcc
     9301 ggagcgcgcg acgatcatgc gcatcccgcc tcaaggcggg ccgtaccagg tgttcgcgcg
     9361 cggggtgcgc aatggcaccg gcctggcgat cgcgcccgac ggttcggtgt ggacggcggt
     9421 gaacaaccgt gacaacatcg cctaccccga cccggggccg tcgtacggcc gcgtgattcc
     9481 ggattacgtc gacgaccacc cgccggagtc gttggcgcgg ttgacctccg gccgcgatct
     9541 cggctggccg tactgcaacc cggccggcgc gggcgggccg gtccc
//
LOCUS       DASWBF010000332         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_954786,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000332 DASWBF010000000
VERSION     DASWBF010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954786"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..723)
                     /locus_tag="VF874_12125"
     CDS             complement(19..723)
                     /locus_tag="VF874_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9176777.1"
                     /translation="MFKLDDDERVITETAAAFAAKRLAPHALEWDAGHHFPVDALREA
                     AELGMAAIYCGEDVGGSGLRRLDAVRIFEQLAAADPTTAAFLSIHNMCAWMVDRYGTH
                     EQRKTWVPKLASMEAVASYCLTEPSAGSDAAALRAKAVREGGDYVLDGVKQFISGRRV
                     RRLCRDGTHGGRRSSGISAFLVENGSAGLSFGAQEQKMGWHAQPTAQVILDGVRVPAD
                     AMLDGAEGDGSGFASR"
     gene            complement(737..>1664)
                     /locus_tag="VF874_12130"
     CDS             complement(737..>1664)
                     /locus_tag="VF874_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9176778.1"
                     /translation="GVFQVVHGDKEAVDALLAHPDIQAYGFVGSSDIAQYIYSNAAAN
                     GKRAQCFGGAKNHMIVMPDADLDQAVDALIGAGYGSAGERCMAITVAVPVGEQTAERL
                     RARLIDRINNLRVGHSLDPKADYGPLVTEAALARVKDYIQQGVEAGAEAVIDGRERTS
                     DDLQFGDANLEEGYFIGPTLFDHVTPDMSIYTDEIFGPVLCIVRADDYEEALRLPSEH
                     EYGNGVAVFTRDGDTARDFVSRVQVGMVGVNVPIPVPVAYHTFGGWKRSGFGDLNQHG
                     PTSIQFYTKVKTVTQRWPAGTHGAEFVIPTMK"
BASE COUNT          252 a          562 c          550 g          300 t
ORIGIN      
        1 gtccgccgtt gagaccgttc atcgcgatgc gaatcctgac ccgtcgccct cggcgccgtc
       61 gagcatcgcg tccgccggca ctcgtactcc gtcgaggatc acctgcgcgg tcggctgagc
      121 gtgccagccc atcttctgtt cctgcgcacc gaaactcagt ccggcagagc cgttttcgac
      181 aaggaacgcc gagatgccgc tggaccgtcg gcccccgtgc gtgccatcac gacatagacg
      241 tcggacgcgc ctgcccgaga tgaactgctt gacgccgtcg aggacgtagt cgccgccctc
      301 gcggaccgcc ttggcgcgca gcgccgcggc gtcggatccg gccgagggtt cggtgaggca
      361 gtagctggcg acggcctcca tcgaggcgag tttcggcacc caggtcttgc gttgctcgtg
      421 ggtgccgtag cggtcgacca tccaagcgca catgttgtgg atcgacagga acgcggccgt
      481 ggtcgggtcg gcggcagcca actgttcgaa gatccgtacc gcatccagcc gccgcagccc
      541 gctgccaccc acgtcctcgc cgcagtagat cgcggccatc cccaactcgg ccgcctcgcg
      601 cagcgcgtcg acggggaagt ggtgaccggc atcccactcc aacgcgtgcg gtgcgagccg
      661 tttggcggcg aacgcggcgg ccgtctcggt gatgacacgt tcgtcgtcgt cgagcttaaa
      721 catattgtgg ccgtgcctat ttcatcgtcg ggatgacgaa ctcggcgccg tgcgtgccag
      781 cgggccaccg ctgcgtgacg gtcttgacct tggtgtagaa ctggatggac gtcgggccgt
      841 gctggttgag atcgccgaat cctgaacgct tccagccgcc gaacgtgtgg taggccaccg
      901 gcaccgggat cggcacgttg acacccacca tcccgacttg cacgcgggac acgaagtcgc
      961 gggcagtgtc accgtcgcgg gtgaaaaccg caacgccgtt gccgtattcg tgttcgctgg
     1021 gcagacgcag cgcctcctcg tagtcgtcgg cccgcacgat gcacagcacg ggtccgaaga
     1081 tctcgtcggt gtagatcgac atgtctgggg tgacgtggtc gaacaaggtg ggaccgatga
     1141 agtagccctc ctccaggttc gcgtcaccga attgcaggtc gtcgctggtg cgttcgcggc
     1201 cgtcgatcac cgcctcggcg ccggcctcga ccccctgctg gatgtagtcc ttgacccgcg
     1261 cgagcgccgc ctcggtgacc agcggtccgt agtcggcctt ggggtcgagg ctgtggccga
     1321 cgcgcaggtt gttgatcctg tcgatcagcc tggcgcgcaa gcgttccgcg gtctgctcac
     1381 cgactggaac cgcgaccgtg atggccatgc accgctcacc ggcgctgccg tagcctgcac
     1441 cgatcaatgc gtcgaccgct tggtcgaggt cggcgtcggg catcacgatc atgtggttct
     1501 tcgcgccgcc gaagcactgc gcgcgcttgc cgttggcggc ggcgttggaa tagatgtact
     1561 gcgcgatgtc cgagctgccg acgaagccgt aggcctggat atccgggtgc gccagcagcg
     1621 cgtcgaccgc ctccttgtcg ccgtgcacca cctggaacac gccc
//
LOCUS       DASWBF010000333        10029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_425322,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000333 DASWBF010000000
VERSION     DASWBF010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425322"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..565)
                     /gene="ssrA"
                     /locus_tag="VF874_12135"
     tmRNA           complement(198..565)
                     /gene="ssrA"
                     /locus_tag="VF874_12135"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(726..1559)
                     /locus_tag="VF874_12140"
     CDS             complement(726..1559)
                     /locus_tag="VF874_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX9176779.1"
                     /translation="MAIQLDKRDVLDGLFGCWDDIDELLAGLSDEQWKAPTALPGWRV
                     HDVVSHIVGTESFLMGVPTPTPDCDVSALPHVNNDLGVMNECWVRHLYDEPPAAMLER
                     FRSVTAERRKTLLAMDDDAWNENTTTPVGPETYGRFMRIRTFDCWMHEQDIRYAVGVW
                     ATDEKVAAAPARHSLAEITAALGRTVGKLGKAPDGSLVAFELTGPLQRTLRVAVEGGR
                     GRVVDEFEDGPTTTIQLDAVLFARLAGGRTTAAENMEAIEISGDVGLGRQVAERLTFV
                     M"
     gene            complement(1764..2261)
                     /gene="smpB"
                     /locus_tag="VF874_12145"
     CDS             complement(1764..2261)
                     /gene="smpB"
                     /locus_tag="VF874_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX9176780.1"
                     /translation="MSKKKPAKSGPPRQIIASNRKARHDYSILDVFEAGVVLVGTEVK
                     SLREGKASLVDAFATVDDGEVWLRNLHIPEYHHGTWTNHAPRRNRKLLLHRSEIDTLI
                     GKIRDGNLTLVPLSLYFVSGKVKVELALARGKQARDKRQDMARRDAQREVTRELGRRV
                     KGMTS"
     gene            complement(2264..3163)
                     /gene="ftsX"
                     /locus_tag="VF874_12150"
     CDS             complement(2264..3163)
                     /gene="ftsX"
                     /locus_tag="VF874_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX9176781.1"
                     /translation="MRFRFLLNEIWTGLRRNVTMTVAMILTTAISIGLFGGALLVWRL
                     AVQSSNIYLSRVESQVFLNADVSQRDPDCKQNPCKALNDLLQSRDDVESVRYLNPDEA
                     YADAIRRIPQFKDVASKQAFPASFIVRLTNPEDDKEFRDSMIAQPGVRDVKNQKDLID
                     RLFAVLYGLRDAAFAVAVVQAIGAILLIANMVQVSAFTRRTEIGIMRLVGASRWYTQL
                     PFLLEAMLAALAGVILAIVGLVFVRAFFLDNALKQFYEANPPLIARVDYLDILYITPY
                     LVVLGVVMAGATAYATLRLYVRR"
     gene            complement(3164..3853)
                     /gene="ftsE"
                     /locus_tag="VF874_12155"
     CDS             complement(3164..3853)
                     /gene="ftsE"
                     /locus_tag="VF874_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX9176782.1"
                     /translation="MITLDKVSKQYKSSARPALDNVSVKIDKGEFVFLIGPSGSGKST
                     FMRLLLAEEKPTKGEIQVSKFHVNHLHGRNIPHLRQVIGCVFQDFRLLQQKTVYENVA
                     FALEVIGKKPEVIKRVVPDVLEMVGLSGKANRLPHELSGGEQQRVAMARAFVNRPLVL
                     LADEPTGNLDPETSKDIVDLLERINRTGTTVVMATHDHHIVDSMRQRVVELDMGKLVR
                     DEQSGVYGMDR"
     gene            complement(3894..4337)
                     /locus_tag="VF874_12160"
     CDS             complement(3894..4337)
                     /locus_tag="VF874_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176783.1"
                     /translation="MTKDNRGWPAYILSGRLRTSTVALIVAFLLVWWIYEANQPEPAP
                     PQAPETQVVPPGFVPDPNYTWVPRTNVWQPTETTTPTPTPTTTETETTSPTDTTTSGP
                     PTTSPTPTSPTPTSPSPTPPAPAGAPTQSPTSAAPGPEPSPTPSR"
     gene            complement(4345..5313)
                     /locus_tag="VF874_12165"
     CDS             complement(4345..5313)
                     /locus_tag="VF874_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX9176784.1"
                     /translation="MTTNTYLALPPADQWHGFWRGQLGEWILTKGLRIVMLLLASLLI
                     ARFITWVAQRISRRIEAGFQETDALVRSETTKHRQAVASVISWVAIAILYVVVAVEIT
                     DILAVPIGSLVAPAAVLGAALGFGAQRIVQDLLSGFFIITERQYGFGDLVELTISGVS
                     NDARGTVEDVTLRVTKLRSSDGEMFTIPNGQIVKSVNLSKDWARAVVNIPVPVTADLN
                     QVNEVLHEVCDSAMEDGQLKDLLLDAPSLMGVESIESERVILRMVARTLPGKQFEVGR
                     RLRVQVVHALSRAGIVSPTETSPTVGAIAHPATAPGAEETQAPESR"
     gene            complement(5303..6409)
                     /gene="prfB"
                     /locus_tag="VF874_12170"
     CDS             complement(5303..6409)
                     /gene="prfB"
                     /locus_tag="VF874_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX9176785.1"
                     /translation="MDPDRQADIAALDSTLTSVERVLDLDGLRARIEKLEHEASDPNL
                     WNDQARAQQVTSELSHAQGELRRVEDLRRRLDDLPVLYELAAEEGSTDELAETDAELK
                     SLRDDIEAIEVRTLLAGEYDEREALLTIRSGAGGVDAADWAEMLMRMYVRWAEKHKYP
                     VEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFAEV
                     EVLPVVETTDHIDIPESDVRVDVYRSSGPGGQSVNTTDSAVRLTHVPTGIVVTCQNEK
                     SQLQNKVSAMRVLQAKLLERKRLEERAELDALKGDGGSSWGNQMRSYVLHPYQMVKDL
                     RTDYEVGNPAAVLDGDIDGFLEAGIRWRNRKDDD"
     gene            6513..7871
                     /locus_tag="VF874_12175"
     CDS             6513..7871
                     /locus_tag="VF874_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9176786.1"
                     /translation="MRPYYVAIVGSGPSGYFAAASLLKLADAAESINVHVDMLEMLPT
                     PWGLVRSGVAPDHPKIKSISAQFEKTALHPRFRFFGNIAVGEHVHAGDLAERYDAVIY
                     AVGTQADKPLRIPGEDLPGSVAATELVGWYNAHPHYKDMPLDLTGERAIVVGNGNVAL
                     DVARILVTDPDELRTTDIADHALEVLDPRGVEEVVIIGRRGPLQATFTTLELRELGDL
                     KGLEGVDVIVDPADVADITDEDAEAAGKTVKNNVKVLRKFAENQPTPGNRRIVFKFRT
                     SPIEIRGDGHVAEVELGRNELVAGEDGRVVAKDTGAREALPAQLVVRAVGYRGVPTPG
                     LPFDDRTGTIPHTDGRIDGSRNEYVVGWIKRGPSGVIGSNKSDSQHTVDTLVADLAEA
                     AHDERHSDALLQWLLSRQPKLVTDEHWQLIDRHERTTGEPHGRPRVKLANLEDLLRIG
                     HG"
     gene            complement(7930..8286)
                     /locus_tag="VF874_12180"
     CDS             complement(7930..8286)
                     /locus_tag="VF874_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176787.1"
                     /translation="MWEFVWIVVVLGALVLLAGPWLMRRRQPADAVNGTLLITGVSPR
                     PDTAGEQFVTVTGVINGPTVNEHVVYQRFAVDVDNWPSMGELIPVVYSPKNPDNWRVA
                     PAGPPDWAPDNAHGGI"
     gene            8310..9098
                     /gene="hisN"
                     /locus_tag="VF874_12185"
     CDS             8310..9098
                     /gene="hisN"
                     /locus_tag="VF874_12185"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX9176788.1"
                     /translation="MGETPDDLALALALADEADALTVPRFGAVDLHVDTKPDLTPVTD
                     ADRSVEEAVRNRLSTECPDDAVVGEEFGGTTEFTGRQWVIDPIDGTKNFVRGVPVWAS
                     LIALLDGGVPVAGVVSAPALHRRWWAAAGHGAYMADGDGAPRRLAVSAVDDLSAASLS
                     FSSLAGWAERGLREKFVDLTDAVWRVRAFGDFYSYCLVAEGAVDVAAEPEVSVWDLAP
                     LDILVREAGGTFTSLDGVPGPHGGSAVATNGWLHEAVLARLSAD"
     gene            complement(9201..10028)
                     /locus_tag="VF874_12190"
     CDS             complement(9201..10028)
                     /locus_tag="VF874_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176789.1"
                     /translation="MGETALTSTGSVIGTWAYMAPERLANGQTDARADIYALTCVLHE
                     CLTGTRPFPGDSLEQQITGHLTVPPPRPSELSADVPAELDTVVATGMAKNPDERYETA
                     KDLARAARAAIIAPTADPSPSVALTEPAPAHHEVSHIAPTQYGPPTGPRPQTPALEPA
                     DNKKPRRRFRIVLAAGIGVIAIVGVVAGVLVINRDKAGDAEPAPSAIPNSGPFTGVFD
                     VEFGPGTDANGAPRENSPPTKETWAIRSVCRDSGCVATAASSTARRSDRQARRQGTT"
BASE COUNT         1611 a         3335 c         3252 g         1831 t
ORIGIN      
        1 cgtcgccagg tcttcgaacg aggtggaggt ctgacgaccg tcatggcgat agagcaccgc
       61 gtgataggcg gtgccgtcct tacggttgcg ggtgcgaaga gaggccatgg cccgctgatc
      121 gtagccgggt ttgttgacgt aagtgttgac gcaaaaaggc ccaggctcgc gattatctcc
      181 ctgacctggg cctttaatgg tggagctgcc gggaatcgaa cccgggtcct acggcatgtc
      241 ctcgaggctt ctccgtgcgc agtccgctat gcctttctcg gatctcccgg tctcacggac
      301 tcgccgggat gacgatccca gtcgctgtta ggtgttccga tgagtcccgc gaccgaactc
      361 atcggtggat ccctctagct gacgccagga tccgggtcga gggagtcccc ggtctgacgg
      421 actagctgtc gcttaggcag cgagaacgta gtcgcgctgt tgtggaacgg cgcttaattg
      481 gttgcaacga cgcttacggt ggtctcttgc ctgcaccggc acgcttccct tgattcgatg
      541 cgcgaagtcg aaaccgttca gcccctcgca tccctgccgg ctttcggcag gacagttcag
      601 tctactcatc gtataacgct cgtccagcgg atttcttcct cgacggcccc gttgagcctt
      661 gagtctgcgc acagggcgtg ggttactcgg cttcgcccag gacgcagagt caacgcgaaa
      721 gcggcctaca tcacgaacgt caggcgctcc gcaacctgcc gccccaggcc cacatcgccg
      781 ctgatctcga tcgcctccat gttctcggct gcggtcgtgc gcccgccggc cagccgcgcg
      841 aacagcaccg cgtccagctg gatcgtcgtc gtcgggccgt cctcgaattc gtcgaccacc
      901 ctcccccggc cgccttccac cgcgacccgc agcgtgcgct gcagcggacc agtcaactcg
      961 aatgcgacga ggctgccgtc tggtgcctta ccgagcttgc cgacggtgcg tccgagggcc
     1021 gcggtgatct ccgcgagaga gtgccgcgcc ggcgcggccg ccaccttctc gtcggtcgcc
     1081 cacacgccca ccgcatagcg gatgtcctgt tcgtgcatcc agcagtcgaa tgtccggatc
     1141 cgcatgaacc ggccatacgt ctcggggccg accggcgtcg tggtgttctc gttccacgcg
     1201 tcgtcgtcca tggccagcag cgtcttgcgc cgctcggcgg tgaccgaccg gaagcgctcg
     1261 agcatcgcgg cgggcggctc gtcgtacagg tgcctgaccc agcattcgtt catcacaccg
     1321 aggtcgttgt tcacgtgcgg cagcgccgac acgtcacagt ccggtgtcgg cgtcgggacg
     1381 cccatcagga acgactcggt gccgacgatg tgcgagacga cgtcgtgcac ccgccagccc
     1441 ggcagtgccg tcggcgcctt ccactgctcg tcagacagcc cggccagcag ttcgtcgatg
     1501 tcgtcccagc agccgaacag tccgtcgagc acgtcgcgct tgtcgagctg aatcgccatt
     1561 ggtcgaccct aaccgctccc tgggggacat accgctgtgc ccgcccctta gtagccctgt
     1621 gtcaacctgc gatgtcagcc atatgttgtc gctgatcgct tcaccgccga taaggcgttg
     1681 acctgggaaa atgacgttgt cgctcggagg tgggcagatc gatgtccggc agctttgcgt
     1741 tgtatgcaat gcgggaacaa cagctacgat gtcatgccct tgacccgacg gcccagctcg
     1801 cgggtgacct cacgttgcgc atcccggcgc gccatgtcct gacgcttgtc gcgggcttgc
     1861 ttgccgcggg ccagcgccag ctcgaccttc accttgcccg acacgaagta cagcgacaac
     1921 ggcaccagcg tcaggttgcc gtcgcggatc ttgccgatca gcgtgtcgat ttcgctgcgg
     1981 tgcaacagca gcttgcggtt gcgccgcggc gcgtggttgg tccaggtgcc gtggtggtat
     2041 tccgggatgt gcaggttgcg cagccacact tcgccgtcgt cgacggtcgc gaacgcgtcg
     2101 accagcgatg ccttgccttc ccgcaggctt ttcacctcgg tgcccaccag caccacgccg
     2161 gcctcgaaca cgtcgagaat cgaatagtcg tggcgcgctt tgcgattcga cgcgatgatt
     2221 tgccgcggcg ggccgctctt cgcaggtttc ttcttgctca cggctagcgc cgcacgtata
     2281 gccgcaaggt cgcgtaggcc gtcgcgcccg ccatcaccac gccgagaacc accaaatacg
     2341 gtgtgatgta gaggatgtcg agatagtcga cgcgcgctat caacggcgga ttggcttcat
     2401 agaactgttt gagcgcgttg tcgaggaaga acgcccgcac gaacaccaac ccgacgatcg
     2461 ccaggatcac gccggcgagc gcggccagca tcgcctccag caggaacggc agctgggtgt
     2521 accacctact ggcgcccacc agtcgcatga tcccgatctc ggttcgccgc gtgaacgccg
     2581 acacttgaac catgttggct atcaacagta tcgcgccgat ggcttgcacc accgcgaccg
     2641 cgaacgccgc gtcgcgcagg ccgtacagca ccgcgaacaa ccggtcgatc aggtccttct
     2701 ggttcttcac atcgcgcacg cccggctggg cgatcatcga atcccggaat tctttgtcgt
     2761 cttcggggtt cgtcaaccgc acgatgaacg aggccgggaa agcttgcttg ctcgccacgt
     2821 ctttgaactg cggaatccgg cggatcgcgt cggcgtaggc ctcgtcaggg ttgaggtaac
     2881 gcaccgactc gacgtcgtcg cgactctgca gcagatcgtt cagcgcctta cacggattct
     2941 gcttacagtc cggatcacgc tgggacacat cggcgttcag gaagacctgc gattccaccc
     3001 ggctcaggta gatgttgctg gactgcacgg ccaaccgcca caccagcagc gcgccaccga
     3061 acaagccgat cgaaatcgcg gtcgtcagga tcatcgcgac cgtcatcgtc acgttgcgac
     3121 gaagaccggt ccatatctcg ttgaggagaa atcgaaagcg cacttagcgg tccatcccgt
     3181 agacgccgct ttgctcgtca cggaccagct tgcccatgtc gagttcgacc acccgctggc
     3241 gcatcgagtc gacgatgtgg tggtcgtggg tggccatcac gaccgtcgtg ccggtgcggt
     3301 tgatccgttc aagcaggtcc acgatgtcct tgctggtctc cgggtctagg ttgccggtgg
     3361 gttcgtcggc cagcaacacc agcgggcggt tcacgaaagc gcgggccatc gccacgcgct
     3421 gctgctcgcc gccggagagt tcgtgcggca gccggttcgc cttgcccgac aggcccacca
     3481 tttccagcac gtcgggcacc acgcgcttga tcacctcggg cttcttgccg atcacctcga
     3541 gcgcgaatgc aacgttctcg tacactgtct tctgctgcag cagccggaag tcctggaaca
     3601 cacagccgat cacctgccgc aagtgcggga tgttccggcc gtggaggtgg ttgacgtgaa
     3661 acttcgacac ctggatctcg cccttggtgg gcttctcctc ggccagcagc agccgcatga
     3721 acgtcgactt gcccgagccg gacgggccga tgaggaagac gaactcaccc ttgtcgatct
     3781 tcacgctgac gttgtcgagc gccggtcggg cggacgactt gtactgcttg ctgaccttgt
     3841 cgagggtgat catcacggca cgccagtgta gcggtgatcc gcgttgggtc cggtcagcgc
     3901 gacggtgttg gcgacggctc gggtccgggt gccgccgatg tcggcgactg cgttggggcg
     3961 cctgccggcg ctggcggcgt ggggctgggc gacgtggggg tgggcgacgt tggggtgggc
     4021 gacgtggtcg gcggaccgct tgtcgtggtg tccgtcggcg acgtcgtctc cgtttccgtc
     4081 gttgtcggag tcggagtggg tgtggtggtt tcggtcggct gccacacatt ggtgcgcggc
     4141 acccatgtgt agttcgggtc gggcacgaaa cccggcggca ccacctgcgt ctcgggggcc
     4201 tgcggaggcg ccggctccgg ctggttggcc tcgtagatcc accacaccag caggaacgcc
     4261 acgatcagcg ccacggtcga ggtgcgcagc cggccgctca ggatgtaggc aggccaacct
     4321 cggttgtctt tggtcatcct gcgatcacct ggactccggg gcctgcgtct cctcggcgcc
     4381 gggagccgtc gccggatggg cgatggcccc caccgtcggg gacgtctcgg tgggcgacac
     4441 gatgcccgcg cggctcagcg cgtgcaccac ctgcacccgc agccgccgtc ctacctcgaa
     4501 ctgtttgccg ggcagcgtgc gcgccaccat gcgcaggatg accctctccg actcgatgct
     4561 ctcgacgccc atcagcgacg gtgcgtcgag cagcaggtcc ttgagctgac cgtcttccat
     4621 cgcggagtcg caaacctcgt gcaggacctc gttcacctgg ttcaggtctg cagtcaccgg
     4681 caccgggatg ttcaccacgg cgcgcgccca gtccttggac aggttcaccg acttgacgat
     4741 ctgcccgttg gggatcgtga acatctcgcc gtcgctggag cgcagcttgg tcacccgcag
     4801 cgtgacgtcc tcgaccgtgc cgcgggcgtc gttggacaca ccgctgatcg tcagctcgac
     4861 caggtcgccg aacccgtact gccgctcggt gatgatgaag aacccgctca acaggtcctg
     4921 cacgatccgc tgcgcgccga aaccgagcgc ggcgcccagc acggccgccg gcgccaccag
     4981 cgacccgatc ggcacagcga ggatgtcggt gatttccacc gcgaccacca cgtacaggat
     5041 cgcgatcgcc acccaagaga tcaccgacgc gaccgcttgt cggtgcttgg tggtctccga
     5101 gcgcaccagc gcatcggtct cctggaagcc cgcctcgatg cgcctgctga tccgctgcgc
     5161 cacccaggtg atgaaccggg caatgagcag cgacgcgagc aagagcatca cgattcgcag
     5221 acccttggtg aggatccact cgccgagctg gcctcgccag aagccgtgcc attggtcggc
     5281 tgggggtaac gccagataag tgttagtcgt catcttttct gttacgccac cggattcctg
     5341 cttcgaggaa cccgtcgatg tctccgtcca gtaccgcagc cggattgccg acctcgtagt
     5401 cggtgcgcag gtccttcacc atctggtacg ggtgcagtac gtaggagcgc atctggtttc
     5461 cccacgaact gccgccgtcg cccttcagcg catccaactc ggcacgttct tctaaacgct
     5521 tgcgttccaa caactttgcc tgaagcacgc gcatcgccga aaccttgttc tgcaactgcg
     5581 acttctcgtt ctggcaggtg accacgatac cggttgggac gtgggtaagt cgaaccgcgg
     5641 agtcggtggt gttgacggat tggccccccg gcccactgga tcggtacacg tcgacgcgga
     5701 catcgctttc cgggatgtcg atgtgatctg tggtctccac cacaggcagc acctcgactt
     5761 cggcgaacga cgtctggcgg cgactctggt tgtcgaacgg actgattcgc accagtcggt
     5821 gcgtgccctg ctccaccgac aacgtcccgt acgcgaacgg ggcgtgcacc gcgaacgtcg
     5881 cgctcttgat gcccgcctcc tcggcgtatg aggtgtcgaa cacctccacc gggtacttgt
     5941 gcttctcggc ccagcggacg tacatccgca tcagcatctc ggcccagtcg gcggcgtcga
     6001 ccccgcccgc accggaccgg atcgtcagca gcgcctcgcg ctcgtcgtac tcgccggcca
     6061 gcagcgtgcg gacctctatc gcctcgatgt cgtcacgcag ggacttcagc tcggcgtctg
     6121 tctcggcgag ctcgtcggtg ctgccctctt ctgccgcgag ctcgtagaga accggcaggt
     6181 cgtcgagccg tcgccgcaga tcctcgaccc ggcgcagctc cccctgagcg tgagacagct
     6241 cactggtcac ctgctgggct ctggcctggt cgttccacag attgggatcg gaggcttcgt
     6301 gttccagctt ctcgatgcgc gcccgaagtc cgtcgaggtc gagaacgcgc tcgacgctgg
     6361 tcagcgtcga gtcgagcgcc gcgatgtcgg cttgccggtc gggatccacg gctgcctagg
     6421 ctacccgccg gtccacgtct accatctgtg ccgtaacccg gccaggtgcg acagccccta
     6481 tcgcacggtc gggcaactgg ggaggctcaa agatgcgtcc ttactatgtg gcgatcgtcg
     6541 gctcaggacc gtccggctat ttcgcggcgg cgtcgctgct gaagctggcc gacgcggccg
     6601 agtcgatcaa cgtgcacgtc gacatgctcg agatgctgcc gaccccgtgg ggcctggtgc
     6661 gctccggcgt ggcgcccgac cacccgaaga tcaagtcgat cagcgcgcag ttcgagaaga
     6721 cggcgttgca cccgcggttc cggttcttcg gcaacatcgc ggtgggcgaa catgtgcacg
     6781 ccggcgatct ggccgagcgc tacgacgcgg tgatctacgc ggtcggcacc caggccgaca
     6841 agccgctgcg gattccgggc gaggacctgc ccgggtccgt cgcggcgacg gagttggtcg
     6901 gctggtacaa cgcgcatcca cactacaaag acatgccact ggatctgacc ggcgagcggg
     6961 ccatcgtcgt cggcaacggc aacgtcgcgc tggacgtggc gcggatcctg gtcaccgacc
     7021 ccgacgagct gcgcaccacc gacatcgccg accacgcgct ggaggtcctc gacccgcgtg
     7081 gggtcgagga agtggtgatc atcggccgtc gcggcccgct gcaggccacg ttcacgacct
     7141 tggagttgcg cgaactcggc gacctcaagg gtctcgaagg ggtcgacgtg atcgtcgacc
     7201 cggcggatgt ggccgacatc accgacgagg atgccgaggc ggccggtaag accgtgaaga
     7261 acaacgtcaa ggtcttgcga aagttcgccg agaaccagcc gacacccggc aaccggcgga
     7321 tcgtgttcaa gttccgcacc tcgccgatcg agatccgcgg cgatggccac gtcgcggaag
     7381 tggagctcgg ccgcaacgaa ctggtcgccg gcgaggacgg acgcgtcgtc gccaaggaca
     7441 ccggcgcgcg tgaggcgctg cccgctcagc tggtggtgcg cgcggtcgga taccgcggcg
     7501 tgccgacccc cggtctgccg ttcgacgatc gcaccggcac gattccgcac accgacggac
     7561 ggatcgacgg cagccgcaac gagtacgtcg tcggctggat caagcgcggc ccgtcggggg
     7621 tgatcggcag caacaagagc gactcgcagc acaccgtcga caccctggtc gccgacctgg
     7681 ccgaagcggc acacgacgag cggcactccg atgcgctgct gcagtggctg ctgtcgcggc
     7741 agccgaagct ggtgaccgac gagcactggc agctgatcga tcgccacgag cggaccaccg
     7801 gcgagccgca cggccggccg cgggtcaagc tggcgaacct cgaggacctg ctgcgcatcg
     7861 ggcacggcta agagggccac gcgtcgcctc actttaaccg cgatcgtgcg aggttctggt
     7921 gaatggcgct cagatgccgc cgtgcgcgtt gtccggggcc cagtcgggcg gtcccgcggg
     7981 tgcgacgcgc cagttgtccg ggttcttcgg tgaatacacg accgggatga gttcacccat
     8041 gctcggccag ttgtcgacat cgaccgcaaa gcgctgatac accacgtgct cgttgacggt
     8101 cggtccgttg atcaccccgg tcaccgtgac gaactgctcg ccggcggtgt ccggccgcgg
     8161 gctgaccccc gtgatcagca gcgtgccgtt caccgcgtcg gcgggctgcc tgcggcgcat
     8221 cagccacggc cccgccagca agacgagcgc cccgagcacc accactatcc atacgaattc
     8281 ccacacgcct tcatggtagg actgctgaca tgggcgagac gccggatgac ttggcgctgg
     8341 cgttggcgct tgccgatgaa gccgacgcac tgacggtgcc gaggttcggc gcggtcgatc
     8401 tgcacgtcga caccaaaccg gacctcacac ccgtcaccga cgccgaccgg tccgtcgagg
     8461 aggccgtgcg caataggctg tcgacggaat gcccggacga cgcggtggtg ggcgaagagt
     8521 tcggcggcac aaccgaattc actggccgcc agtgggtgat cgacccgatc gacggcacca
     8581 agaacttcgt gcgcggtgta ccggtgtggg cgagccttat cgctctgctc gacggcggtg
     8641 tgccggtcgc cggggtggtc agcgcgccgg cgctgcatcg gcggtggtgg gcagccgcgg
     8701 gtcacggcgc atacatggcc gacggagatg gtgcccctcg acggctcgcg gtgtcggcgg
     8761 tcgacgacct gtcggcggcg agcctgtcgt tctccagcct ggccggatgg gccgaacgcg
     8821 gactgcggga gaagttcgtc gacctcacgg acgctgtgtg gcgggtgcgc gccttcggtg
     8881 atttctactc gtactgtctg gtggccgaag gcgccgtcga cgtcgccgcc gaacccgaag
     8941 tgtccgtgtg ggatctggcg ccgttggaca tcctggtccg agaagcgggc ggcacgttca
     9001 ccagcctgga cggcgtgccg ggcccacacg gcggcagtgc ggttgcgacc aacggctggt
     9061 tgcacgaggc cgtgctcgca cgactttctg ccgactagtc ggccagtcag tcgaattctg
     9121 ttgcagcgga agatgatcaa tcgccagtgc ggtcgaaggt cgcatcgaaa tccgaagaag
     9181 cgcatgccgt gttgctgtcg tcatgtcgtc ccttgccgac gagcttggcg atcggatctt
     9241 ctggcggttg aggaagcggc tgttgcgaca cagccgctgt cacggcacac cgaccggatc
     9301 gcccaggttt ccttggtcgg cgggctgttc tcgcgcggcg cgccgttcgc gtccgtgccg
     9361 ggaccgaatt ccacatcgaa caccccggtg aaaggcccgc tgttgggaat ggcgcttggg
     9421 gctggttctg catcaccggc tttgtcgcgg ttgatcacca gaacgcctgc caccacgccg
     9481 acgatcgcaa tcacgccgat gcccgccgcg agcactatgc gaaatcgcct gcgcggcttc
     9541 ttgttatcgg ccggctcaag cgcaggcgtc tgcggtctgg gacctgtggg tggcccgtac
     9601 tgggtgggcg cgatgtgcga cacctcgtgg tgcgcgggag cgggctcagt cagcgccacc
     9661 gaggggctcg ggtccgcagt cggtgcgatg atcgccgcgc gtgccgctct cgcgaggtct
     9721 ttcgcagttt catagcgctc atctgggttc ttcgccattc ccgtggcaac aacggtgtca
     9781 agttcggccg gcacatcggc gctcagctcc gacgggcggg gcggcggcac cgtcaggtgc
     9841 cccgtgatct gctgttccag actgtcgcct ggaaacggtc gagtgccagt tagacattcg
     9901 tgcaagacac acgtcagtgc atagatgtcc gctcgggcgt cggtctgtcc gttggccagt
     9961 cgctcaggcg ccatgtacgc ccacgtgccg atgacgctgc cggtactggt caatgccgtc
    10021 tcccccaac
//
LOCUS       DASWBF010000334         7614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_690807,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000334 DASWBF010000000
VERSION     DASWBF010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690807"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1332
                     /locus_tag="VF874_12195"
     CDS             205..1332
                     /locus_tag="VF874_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9176790.1"
                     /translation="MTLSDSERAELIRRARFCTLPHEQVVRAKIVLLAADGVSNTAIA
                     ERVDVHVDVVSRWRKRFCEQRLDGLSDRKRSGRPRVFAAEVVVEVKAMACQPPEQRQV
                     PLSRWSSAELAAQAVTDGLVESVSASTVRRWLAEDAIQPWRYRSWIFPRDPNFAVKAG
                     RVLDLYERVWEGIALGADEYVISADEKSQSQALSRCHPELAPAPGRTRRVEFEYDRHG
                     TLAYFGAYDVHRAHLSGRIAPSTGIAPFTELVEQVMTTEPYATAKRVFWIVDNGSSHA
                     GQASIERMSAAWPSATLVHLPVHASWLNQIEIVFSVIQRKVVKPADFADLGALAHRLT
                     DFEPRYNATATPFDWRFTRTDLIELCHRIDAHRPANPVLVG"
     gene            1352..1711
                     /locus_tag="VF874_12200"
     CDS             1352..1711
                     /locus_tag="VF874_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176791.1"
                     /translation="MPTPPASTKSSLTFTLKDHARARWPQLVGVRVRFRAGFAYVDGD
                     LGDGEVLKLCRLRYGGSASRWGFAIYRASHDDYEDSILPNGAFAGSPEQALDCACGLY
                     LGDPTAWTETVTDTPTN"
     gene            1915..2130
                     /locus_tag="VF874_12205"
     CDS             1915..2130
                     /locus_tag="VF874_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176792.1"
                     /translation="MWIQIKDHDEDDQYSDDATYEVLTSGVLMVTSGNDIHLYSPAYW
                     QEVTIDTRSADQRDKQVPQLDEDLKWQ"
     gene            2121..2309
                     /locus_tag="VF874_12210"
     CDS             2121..2309
                     /locus_tag="VF874_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176793.1"
                     /translation="MAMMAGGRRNSKRGEGELALANGVTTAERREYTEAVLGLQMYNH
                     HLYKSMRRNPHEAERASV"
     gene            2329..2454
                     /locus_tag="VF874_12215"
     CDS             2329..2454
                     /locus_tag="VF874_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176794.1"
                     /translation="MTNGRFLEAIKRLLAAETYFRSREEAEEEREPERPAPEGDK"
     gene            complement(2464..3069)
                     /locus_tag="VF874_12220"
     CDS             complement(2464..3069)
                     /locus_tag="VF874_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9176795.1"
                     /translation="MQLHKSDVVEAATKLLDTYGIADLTMRRLARELDVSPGALYWHF
                     PNKQALLGAVADRVLQPVDDVAGSWRDRVTGTCARLRDALLSHTDGAELVSASFAAGQ
                     SAAMASIVTQLACAAGEAGVALGKAELAARTVVYYVLGFTADEQSRLQWDAAGALADE
                     QSVLTTDPSGRFAFGLELLVDGIAHAGAETAGSAPLRGDGP"
     gene            3122..4117
                     /gene="bioB"
                     /locus_tag="VF874_12225"
     CDS             3122..4117
                     /gene="bioB"
                     /locus_tag="VF874_12225"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX9176796.1"
                     /translation="MTDVLEQARERVLERGEGLTRDQVLAVLRLGDERLDELLALAHE
                     VRMRWCGPEVEVEGIISLKTGGCPEDCHFCSQSGLFSSPVRSAWLDIPSLVEAAKQTA
                     KTGATEFCIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLTQQQVDQLSAMG
                     VHRYNHNLETARSFFGNVVTTHTWEERWDTLSMIRDAGVEVCCGGILGMGETLEQRAE
                     FACQLAELDPDEVPLNFLNPRPGTPFGDLDVLPASEALKAVAAFRLALPRTMLRFAGG
                     REITLGDLGAKRGILGGINAVIVGNYLTTLGRPAEADLELLDGLQMPLKALNATL"
     gene            4122..4322
                     /locus_tag="VF874_12230"
     CDS             4122..4322
                     /locus_tag="VF874_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176797.1"
                     /translation="MPAKFNVYTGEPAGTAVPRAARLGLEPPRYCADCGRRMTVQVRP
                     DGWSAKCSRHGAVDSTDMETKR"
     gene            4319..4870
                     /locus_tag="VF874_12235"
     CDS             4319..4870
                     /locus_tag="VF874_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2567 domain-containing protein"
                     /protein_id="HEX9176798.1"
                     /translation="MKRWLGVSAGMAVAGVAAGALWAWLAPPIHSAAAVTKSGKRVQQ
                     YLGNEADHYFDAAVMMAGLLVGLGVVAAVLVWQWRHRRGPGMVIGLTAGGLAAAAAAA
                     GTGAGLVRIRYGHFDVDAVPGDHKLHYITEAPAVFFGHAPLQVLTMLLLPAATAALTY
                     AMMAAASAHDDLGVESSVPETVG"
     gene            complement(4847..5965)
                     /locus_tag="VF874_12240"
     CDS             complement(4847..5965)
                     /locus_tag="VF874_12240"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX9176799.1"
                     /translation="MRTAICDMLGIDIPLLAFSHCRDVVAAVTNAGGMGVLGAVTLTP
                     EQLEQELNWIESEVRGKPYGVDIIVPETSVGKGQRLSAEQFADMIPAEHRAYVDALLA
                     DHGITPVTNRLPRVATTDDAGESLLEVAFTHPIKLIDNALGRPPQSMLDAGKSHGVPV
                     AALAGAKEHAIKHVNAGVDVIVAAGTEAGGHCGDVTTMVLVPEVIEAIEPIRPVPVLA
                     AGGIVTGRQMAAAVAMGAAGAWTGSVWLTTEEAETAPYTKQRMLTATSRDTIRSTCRT
                     GKPTRQLKSDWTEAWESGQRPEPLPMPLQSLISEPVLCRIDMLAANGDEGARALATYW
                     VGQGVGLMNSVKPARQVVYEFAQDYLAAAERLADSLGD"
     gene            complement(5990..7339)
                     /locus_tag="VF874_12245"
     CDS             complement(5990..7339)
                     /locus_tag="VF874_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX9176800.1"
                     /translation="MDSAGLARAAGAEWIGAVPHEELRRSRPVLPHQDPFYEPPPGFE
                     HSAPGTVLRTRDVELGFLGFVPQKFFATQLLYRSSDRKGAPEAAVTTVLVPTERGPEN
                     VCPIVSYQCAIDAVTDRCFPSYAMRRRAFAPGALAQFEFLLVAAALAEGWAVSVPDHE
                     GTHGMWGAPYEPGYRILDGLRAATSCERLGISADSPIGLWGYSGGGLATAWAAETCAD
                     YAPELNIVGAVLGSPVGDLGHAFKRLNGSFYAGLPTMVIAALWRIYPELREVIDRHAT
                     DEGRALLKSAEKMTTVEAVLRLMNKDIDAYVDRPLEDILAEPEVQFVFDDIKLGTAAP
                     SRPVLIVQAVHDTIVSVDDIDELTESYRAGGASVTYHRDMFSEHMLLHPMSAPMTLRW
                     LRDRFAGRPLSEHLVRTTWPTLFNPSTYKGMMRLAGIAARVITGRKVERTPLSYTDA"
     gene            complement(7402..>7614)
                     /locus_tag="VF874_12250"
     CDS             complement(7402..>7614)
                     /locus_tag="VF874_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9176801.1"
                     /translation="STLRDIYDAALGYQVDATNLQRVLDRRNVITRTGTIARSGRSGG
                     RPAALYRFTDSLYRVTDEFAALRPPG"
BASE COUNT         1264 a         2580 c         2554 g         1216 t
ORIGIN      
        1 ggctgcccca gcagcagaac cgtcgcggtc aacagcatca gaaactacag tgagcatggg
       61 tcgtaccttc ccgaccagcg ttaccgcgcc ggtcttgctt ggaaccttta ttcggtcacc
      121 cgggaaggta cgccccttcc caagccgatc cacagatttc aagcattgct caggctcgcc
      181 gtgtcccgat cgtctccgtt tgtggtgacg ttgtccgatt ccgagcgtgc cgagttgatc
      241 cgccgcgccc gcttttgcac gctgccgcac gagcaggtgg ttcgggccaa gatcgtgctg
      301 cttgccgccg acggcgtgtc caacaccgcg atcgccgagc gggtcgatgt gcacgtcgac
      361 gtggtgagcc ggtggcgcaa gcgattctgc gagcaacgat tggacgggct gtccgatcgg
      421 aagcggtcgg gccggccgcg ggtattcgcg gcggaggtgg ttgtcgaggt gaaggcgatg
      481 gcgtgccagc cgccggagca gcggcaggtg ccgctgtcgc ggtggagctc ggccgagctg
      541 gccgcgcagg cggtcaccga tggcctggtc gagtcggtgt cggcttccac ggtgcggcgc
      601 tggctggccg aggacgcgat ccaaccgtgg cggtaccggt cgtggatctt cccgcgggac
      661 cccaactttg cggtcaaggc cggtcgggtg ctggatttgt acgagcgggt gtgggagggc
      721 attgcgctcg gcgcggacga gtacgtgatc agcgccgacg aaaagagcca gtcgcaggcg
      781 ctgtcgcggt gtcatcccga actcgcgccc gcaccggggc ggacacggcg ggtcgagttc
      841 gagtacgacc ggcacgggac cctggcctat ttcggcgcct acgacgtgca tcgcgcacac
      901 ctgagcgggc ggatcgcacc gagtaccggg atcgccccgt tcaccgagct ggtcgagcag
      961 gtgatgacca ccgagcccta cgcgacggcc aaacgggtgt tctggatcgt ggacaacggc
     1021 tccagccacg ccgggcaggc ttcgatcgag cggatgagcg cggcgtggcc gagcgcgaca
     1081 ctggtgcacc tgccggtgca cgcgtcctgg ttgaatcaga tcgagatcgt gttctcggtg
     1141 atccaacgca aggtcgtcaa gccggccgac ttcgccgacc tgggtgcgct cgcgcaccga
     1201 ctgaccgact tcgagccgcg ctacaacgcc accgccacac cgttcgactg gaggttcacc
     1261 cgcaccgacc tgatcgaact gtgccaccgc atcgatgccc accgacccgc caatccggtt
     1321 ctggtgggat gactttggag atgatgatcc gatgccgacc ccgcccgcgt cgacgaagtc
     1381 cagcctcacc ttcacactga aagaccacgc ccgcgctcgc tggccgcagc tggtcggcgt
     1441 gcgggtacgt ttccgcgccg gattcgccta cgtcgacggc gacctcggcg acggcgaggt
     1501 actgaaacta tgccgactgc gctacggcgg ctcggcgagc cgatggggat tcgcgatcta
     1561 ccgagccagc cacgacgact acgaagacag catcctgccc aacggagcat tcgccggcag
     1621 tccagagcag gccctcgact gcgcctgcgg actctacctc ggcgacccca ccgcctggac
     1681 cgagacggtc accgatacac cgacgaacta accagcagga ccacctcctt tgcagtcgtc
     1741 gaaccagata cagaatcgac cccgttatgc ctgctcgcca gcaggcgaaa aacctcgttc
     1801 tcgggacttc cgaccacgga taaacgggac ctccgactcc gggcatctcg ccataacgcc
     1861 ggtagcgtca agaatcccga tctgaagatg cggaggatta ctctcattat gaaaatgtgg
     1921 atacaaatca aagaccacga tgaggatgac caatattcgg acgatgccac ctatgaagta
     1981 ctcacgagcg gtgtgctaat ggtcaccagc ggcaatgata ttcacctcta cagtccggcg
     2041 tactggcaag aggtcacgat tgatacccgc tccgcggatc agcgtgacaa acaagtccca
     2101 caattggacg aagacctcaa atggcaatga tggccggggg gcggcgcaat agtaagcgcg
     2161 gagaaggcga attggccctg gccaacggcg tgacgacggc ggaacgtcgg gagtacaccg
     2221 aagccgtttt gggactgcag atgtacaacc atcacctgta caagagcatg cggcgaaatc
     2281 cgcacgaggc ggaacgcgcg tcagtttgag gtctttagag gagtcgcaat gactaacggc
     2341 cgttttctcg aagccatcaa gcgcctgctc gctgcggaga cgtacttccg ttcgcgcgag
     2401 gaggccgagg aggagcgtga gccggaacgt ccggcccccg agggggacaa gtgaccccgg
     2461 agcctacggg ccgtcaccgc gcagcggcgc gctgcctgca gtctcggcgc cggcatgtgc
     2521 gatcccgtcg acaagcagtt ccagcccgaa cgcgaaacgt ccgctgggat ccgtcgtcaa
     2581 caccgattgc tcgtcggcta gcgctccggc ggcgtcccac tgaagccgtg actgctcgtc
     2641 cgcggtgaag cccagcacgt agtacacgac ggttcgggcc gccaactcgg ccttcccgag
     2701 tgccaccccg gcctcgccag ccgcacaagc aagctgcgtg acgatcgacg ccatcgccgc
     2761 ggactgcccg gcggcgaagc tcgccgacac cagctccgcg ccgtcggtgt gcgacagcag
     2821 cgcgtcgcgc agccgcgcgc atgtcccggt gacacggtcg cgccagctgc cggcgacgtc
     2881 gtcaaccggc tgcaagaccc ggtcggccac cgcgccgagc agcgcctgct tgttggggaa
     2941 gtgccagtac agcgcgcctg ggctgacgtc gagctcgcgg gccagccgtc gcatcgtcag
     3001 atccgcgatg ccgtaggtgt ccagcagctt tgtcgccgcc tcgaccacgt cgcttttgtg
     3061 cagctgcacc cacgtaccct aacctgaaca ccgttcaagt ccgcaccgtg aggagctcga
     3121 ggtgaccgac gtcctggaac aggcccgcga acgggtgctg gagcgcggcg agggtctgac
     3181 gcgggaccag gtgctggctg ttctgcggct gggcgacgag cggctcgacg agttgctggc
     3241 gctggcccac gaggtgcgca tgcgctggtg cgggccggag gtcgaggtcg agggcatcat
     3301 cagcctcaag accggcggct gcccggagga ctgccacttc tgttcgcagt ccgggctgtt
     3361 cagctcgccg gtgcgcagcg cgtggctgga catcccgagc ctcgtcgagg ccgccaagca
     3421 gaccgcgaag accggcgcca ccgagttctg catcgtcgcc gccgtgcgcg gacccgacga
     3481 gcggctgctg gcgcaggtcg ccgccggcat cgaggcgatt cgcaacgagg tcgacatcca
     3541 gatcgcgtgc tcgctgggca tgctgaccca acagcaggtc gaccagctct ccgcgatggg
     3601 cgtgcaccgc tacaaccaca acctcgagac cgcgcggtcg ttcttcggaa acgtggtgac
     3661 gacgcacacc tgggaggagc gctgggacac gctgtcgatg attcgtgacg cgggtgtgga
     3721 ggtgtgctgc ggcggcatcc tcgggatggg cgaaaccctg gagcaacgag ccgaattcgc
     3781 ctgccagctc gccgaattgg atcccgacga agtgccgctg aacttcctca acccgcggcc
     3841 cggcaccccg ttcggcgacc tcgacgtgct gcccgcgtcg gaggcgctca aagccgtcgc
     3901 cgcattccgg ctcgccttac cgcgcacgat gctgcggttc gccggcggcc gggagatcac
     3961 gctcggcgac ctcggcgcca agcggggcat tctcggcggc atcaacgcgg tgatcgtcgg
     4021 caactacctc accacgttgg ggcggcccgc cgaggccgac ctcgagttgc tcgacggcct
     4081 gcagatgccc ctcaaggcgc tcaacgcgac gctgtagagc catgccggcc aagttcaacg
     4141 tctacaccgg cgaacccgcg ggcacggcgg tgcctagggc cgcacgactc ggtctggagc
     4201 cgccccgcta ctgcgctgac tgcggtcggc ggatgaccgt gcaagtgcgt cccgacgggt
     4261 ggtcggcgaa gtgctcccgc cacggggcgg tggactcgac ggacatggag accaagcgat
     4321 gaagaggtgg ctcggggtgt cggccgggat ggccgttgcc ggtgtggcgg ccggcgcgct
     4381 gtgggcgtgg ctcgccccgc cgatccacag cgccgccgcg gtgacgaagt ccggcaagag
     4441 ggttcagcag tacctcggca acgaggccga ccactatttc gacgcggcgg tgatgatggc
     4501 cggcctgctg gtcgggctgg gtgtggtggc cgcggtgctg gtctggcagt ggcggcaccg
     4561 gcgcggccct gggatggtga tcggtttgac ggccggcggg ctggcagccg cggccgcggc
     4621 cgccggcacc ggagccgggc tggtgcgaat tcgctacggc cacttcgacg ttgacgcggt
     4681 tccgggcgat cacaagcttc actacatcac cgaggcgccg gcggtgttct tcgggcatgc
     4741 accgctgcag gtgctgacca tgctgctgct gcccgccgcg acggccgccc tcacctacgc
     4801 gatgatggcc gccgcatccg cgcacgacga cctcggcgtc gagtcgtcag tccccgagac
     4861 tgtcggctag ccgctcggcc gccgccaggt aatcctgcgc gaattcgtag accacctgcc
     4921 gcgcgggctt gacggagttc atcaggccga cgccctgacc cacccagtag gtggccagcg
     4981 cgcgagcgcc ctcgtcgccg ttggcggcca gcatgtcaat gcgacacagt accggctcgc
     5041 tgatcaacga ctgcagcggc attggcagcg gctcgggtct ctgcccggac tcccatgcct
     5101 cggtccaatc ggacttcagc tggcgcgtcg gcttgccggt gcggcacgtc gaccgaatcg
     5161 tgtcgcgcga cgtcgccgtc agcatccgct gcttggtgta cggcgcggtc tcggcctcct
     5221 cggtggtgag ccacacggat ccggtccacg caccggcggc gcccatcgcc accgcggccg
     5281 ccatctgccg cccggtcacg attccaccgg cggccagcac tgggaccggc cggatgggtt
     5341 cgatggcttc gatgacctcg ggtaccagca ccatcgtcgt cacgtcgccg cagtgcccgc
     5401 cggcctcggt gccggccgcg acgatgacgt cgacgcccgc gttgacgtgc ttgatggcat
     5461 gctccttcgc gccggccagc gccgcgacgg gaacgccgtg cgacttgcct gcgtccagca
     5521 tcgactgcgg cggccggccc agcgcattgt cgatcagctt gatcgggtgc gtgaaggcga
     5581 cttccaacag gctctcgccg gcgtcgtcgg tggtcgccac acgcggcagc cggttggtca
     5641 ccggcgtgat gccgtggtcg gccaacagcg cgtcgacata cgcgcgatgt tcggcgggga
     5701 tcatgtccgc gaactgctcg gcgctgagcc gctggccctt gccgaccgat gtctccggca
     5761 cgatgatgtc gactccgtac ggcttgcccc gaacctcgga ctcgatccaa ttgagttcct
     5821 gctcaagctg ttcgggggtc agcgtcaccg cgccgagcac acccattccg ccggcgttgg
     5881 tgaccgcggc gaccacgtcg cggcagtggc tgaacgcgag caacgggatg tcgatcccca
     5941 acatgtcgca gatcgcagtt ctcatggcca ctccctcggc ccggtcgagc taggcgtcgg
     6001 tgtacgacag gggtgtgcgc tccaccttgc ggccggtgat cacccgcgcc gcgattcccg
     6061 ccagccgcat catgcccttg taggtggacg ggttgaacag ggtgggccac gtcgtgcgca
     6121 ccagatgctc cgacaacggc cggccggcga atcggtctcg cagccagcgc agcgtcatcg
     6181 gcgccgacat cgggtgcagc agcatgtgct cgctgaacat gtcccggtga taggtcaccg
     6241 acgcgccgcc cgcgcggtag ctctcggtca gctcgtcgat gtcatcgacg gacacgatcg
     6301 tgtcgtgcac ggcctgcacg atcagcaccg ggcgactggg tgcggccgtg ccgagtttga
     6361 tgtcgtcgaa cacgaactgg acctcgggct cggccaagat gtcctcgagc ggacggtcga
     6421 cgtaggcgtc gatgtccttg ttcatcaacc gcagcacagc ttcgaccgtg gtcatcttct
     6481 ccgccgactt cagcagcgct cggccctcgt cggtggcgtg ccggtcgata acttcccgca
     6541 gctccggata aatgcgccag agtgcggcga tcaccattgt cggcagcccg gcgtagaacg
     6601 agccgttgag ccgcttgaac gcgtgcccga gatcgccgac cggcgacccc agcaccgcgc
     6661 cgacgatgtt gagttcgggc gcgtagtccg cgcaggtctc ggccgcccag gcggtcgcga
     6721 ggccaccccc ggaataaccc cagaggccga tcggcgagtc ggcggagatg ccgagccgct
     6781 cacaacttgt ggcagcgcgc agcccgtcca ggatccggta gcccggctcg tagggcgccc
     6841 cccacattcc atgagtgccc tcgtggtcgg gcaccgacac cgcccagccc tccgcgagtg
     6901 cggcagccac gagcaaaaac tcgaactgcg ccagcgcgcc gggcgcgaac gcccggcggc
     6961 gcatcgcgta cgaggggaag cagcggtcgg tcaccgcgtc gatcgcgcac tgatacgaca
     7021 cgatcggaca gacgttctcc gggccccgct cggtgggcac cagcacggtg gtcaccgcgg
     7081 cttccggcgc gcctttgcgg tcgctgctgc ggtacagcag ctgcgtggcg aagaacttct
     7141 gcgggacgaa cccgagaaac cccagctcca cgtcgcgcgt gcggagcacc gtgcccggtg
     7201 cggagtgctc gaagcccggg ggcggttcgt agaacgggtc ttggtgaggc agtaccggcc
     7261 ggctccgccg caactcctca tgcggcacag cgccgatcca ctcagcaccg gccgcgcggg
     7321 ccaggccggc agaatccatg tgaacactcc ttcgtgcccg accgcattct tccttaaaag
     7381 gccctaaaga agccagccga ttcacccggg cgggcgcagt gcggcgaatt cgtccgtgac
     7441 gcggtacagc gagtcggtga accggtagag cgccgccggg cgacctccgc tgcggccgct
     7501 gcgggcgatc gtgccggtgc gggtgatgac gtttcggcgg tcgagcaccc gctgcaggtt
     7561 ggtggcatcg acctggtagc ccaacgccgc gtcgtagatg tcccgcagcg tcga
//
LOCUS       DASWBF010000335         6096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_955245,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000335 DASWBF010000000
VERSION     DASWBF010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955245"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..393
                     /locus_tag="VF874_12255"
     CDS             130..393
                     /locus_tag="VF874_12255"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9176802.1"
                     /translation="MTSGSIPISELREQLAAVIDELGVHGKAIKLTRHGQPVAVLMGF
                     EAFEALLDDLDDADDYRALAEHDATDERDIIPWELVKHDLGIA"
     gene            390..653
                     /locus_tag="VF874_12260"
     CDS             390..653
                     /locus_tag="VF874_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9176803.1"
                     /translation="MTYRVHKTLRVDRELRGIQKRDRLRIEGVVALLAENPRPPKATK
                     LVGFRNRWRVRTGDYQILYEIDDDVLTVPVIRVAHRRQVYRDS"
     gene            complement(659..1408)
                     /locus_tag="VF874_12265"
     CDS             complement(659..1408)
                     /locus_tag="VF874_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9176804.1"
                     /translation="MTRQKILITGASAGLGAGMARAFAAKGRDLALCARRTDRLDELK
                     AELTAQYPNIKVAVAELDVNDHEQVPKVFAALSDELGGIDRVIVNAGIGKGWPLGEGK
                     LWANKATIETNLVAALVQIETALEMFKNTGSGHLVLISSVLGNKGVPGVKAAYAASKA
                     GLSSLGESLRAEYASGPIKVTVIEPGYIESEMTAKSGSTMLMVDNQTGVRALVNAMER
                     ERGHAIVPAWPWAPLVQLLKVLPPRLAKPFA"
     gene            complement(1496..1963)
                     /locus_tag="VF874_12270"
     CDS             complement(1496..1963)
                     /locus_tag="VF874_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX9176805.1"
                     /translation="MPGIAALGPDALDLSPDDLAGLLAGNSGRLKSVITDQKVIAGIG
                     NAYSDEILHVAKLSPFATAGKLAVEQVSALHDAMISVLTDAVARSVGQQAATLKGEKR
                     SGLRVHDRTGLPCPVCGDTVREVSFADKSFQYCPTCQTGGKVLADRRLSRLLK"
     gene            complement(2093..2350)
                     /locus_tag="VF874_12275"
                     /pseudo
     CDS             complement(2093..2350)
                     /locus_tag="VF874_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
     gene            complement(2366..2764)
                     /locus_tag="VF874_12280"
     CDS             complement(2366..2764)
                     /locus_tag="VF874_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX9176806.1"
                     /translation="MGSFLLRAVLTGFALWVVTLLVGGISFVGGNTTLQKIGIIFVVG
                     VIFGLVNAFVKPLVQLLSIPLYILTLGLIHIVINAFMLWITAWITEHTTRWGLYIDHF
                     WWTAIWAAILLSIVSWLLGLVTRDVTSARQ"
     gene            complement(2967..3710)
                     /gene="cobF"
                     /locus_tag="VF874_12285"
     CDS             complement(2967..3710)
                     /gene="cobF"
                     /locus_tag="VF874_12285"
                     /EC_number="2.1.1.152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043819 - precorrin-6A synthase
                     (deacetylating) activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A synthase (deacetylating)"
                     /protein_id="HEX9176807.1"
                     /translation="MTRRIHVIGIGAGDPDYVTAQAVSVLNDTDVFFVMDKGEAKSDL
                     VAWRRDICARFIDPSRRYRFVELRDPARAPTGDYTAAVSDWHAARARLWAGAIEASLA
                     PGEVGAFLAWGDPSLYDSTLRILDAVAQHVDISYDVVPGITAIQALTARHRLPLNDIG
                     EPVLITTGRKLDPGFRGTVVVMLDGDCAFRKCPPDTLIWWGAYLGTEHELSIAGTVGD
                     VGERIVALRADARARHGWIMDTYLLRSAD"
     gene            3835..4731
                     /locus_tag="VF874_12290"
     CDS             3835..4731
                     /locus_tag="VF874_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HEX9176808.1"
                     /translation="MTTPTKATTQEEFSARLLNGSVKKSYEPVVDIDWDAPLDPDKFY
                     LPPKVVSLYGTPMWDAMTREQQIELSRQEFVNTLSAGIWFENMLNQSLLRHILHRDPT
                     ANATHYALTELGDETRHMVMFGKAIERVGARPVQPRRYQRWVINSLPLAFRGAFMWVA
                     ALIGEEIFDSLQRQMMDDPALQPMVQRLMRIHVTEEARHIQFARDGVRKRVGEMPWVS
                     RFWVANINGLGGVFFRYLFTNPVPYNRVGLDGREARRVARNNPNYYEVQRIGFAPLAA
                     FLQDVGLMGPIARRLWKRTKFL"
     gene            4760..5761
                     /locus_tag="VF874_12295"
     CDS             4760..5761
                     /locus_tag="VF874_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010924322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4873 domain-containing protein"
                     /protein_id="HEX9176809.1"
                     /translation="MIDVAVIGADDDVVAANFDDATNTWLLRTSSGGERRARVVIDER
                     TLHLAAEWDPAFDPTGKRIAVIGDRAAHVLPKLAGAQATLFDCPATFQTRNTKRRWLP
                     RRPPGRPHLVTSPVAHITSKGVRTTDGTEHVVDAIIYATASTTKTGLPDDALVGANGL
                     TIQQAWRDAATAYYGVAVHGFPNYFLLHGPDSPIDNDQVRYIADCLGLMKHHNATRIE
                     VRRSAQQQYAQRVRIKPPTMAFDLTDEAHDIYDGPATLSLGDDEHTVHAQLTGHLDPI
                     DGKYHWQGTVLGAQVDLPKRPVAIATEARSAQARITEQTPWGSYSIAGIGAPPYELT"
     gene            complement(5746..>6096)
                     /locus_tag="VF874_12300"
     CDS             complement(5746..>6096)
                     /locus_tag="VF874_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9176810.1"
                     /translation="AVGFCFGGGMVWQLLASGEPSLAAAVPFYGPLPDNPDFSGSKAA
                     VLAIYGALDARVNASKDAAVAALQHAGLVHDVYVAPDADHAFFNDTGSRYNPAAAAEA
                     WRRVLDWFGRYVSS"
BASE COUNT         1174 a         2080 c         1876 g          966 t
ORIGIN      
        1 cagaacgcgg ccgaactgcc tgactaccgc gccgaaactg tattttcaca gaagaagttc
       61 gagtaacggc ctgccggaat acagtttcgg cgcatcaggc gttctgtaca acgttgtaca
      121 attgtcgcca tgacgtcagg cagcattccc atatcggaac tgcgcgaaca attggcagcg
      181 gtgatcgacg aactcggcgt gcacggcaaa gcgatcaagc tgacgcgcca cggccaaccc
      241 gtcgcagtgc tgatgggctt tgaagcattc gaggcgctgc tcgacgatct cgatgacgcc
      301 gacgactacc gcgcgcttgc cgaacacgat gcgactgacg aacgcgacat aatcccttgg
      361 gaattggtga agcacgatct cggaatcgcg tgacctatcg ggtccacaag acgcttcggg
      421 ttgaccgcga gctgcgaggg attcagaagc gcgaccgact ccgtatcgaa ggtgtcgttg
      481 cattgctggc cgagaatcct cgaccgccca aggcgacgaa gctcgtcggc ttccgcaacc
      541 gatggcgggt gcgtacaggc gattaccaga ttttgtacga aatcgacgac gacgtcctga
      601 ccgtgccggt gatccgggtg gcgcaccggc ggcaggtcta ccgcgactcg tagcggcctc
      661 aggcgaacgg tttggccagc cgcggcggca acactttgag cagctgcacc agcggcgccc
      721 acggccaagc cggaacaatt gcatgtccgc gttcgcgttc catcgcgttg accaacgcgc
      781 gcacgccagt ctggttgtcc accatcagca tcgtgctgcc cgatttcgcg gtcatctccg
      841 attcgatgta acccggctcg attacggtca ccttgattgg gccgctcgca tactcggcgc
      901 gcagggattc gcccagcgac gacagcccgg ccttgctggc ggcgtacgcg gccttgacgc
      961 cgggcacgcc cttgttgccg agcaccgacg agatcaacac cagatgacca gaacccgtgt
     1021 tcttgaacat ctccagcgcg gtctcgatct gcaccagcgc cgcgaccaga ttggtctcga
     1081 tcgtggcctt gttcgcccac agcttgccct cgcccaacgg ccagcccttg ccgattccgg
     1141 cgttgacaat cacgcgatcg atgccgccga gttcgtcgga cagcgcggcg aacaccttgg
     1201 gcacctgctc gtggtcgttg acgtcgagct cggcgaccgc caccttgatg ttcgggtatt
     1261 gcgcagtcag ctcggctttg agctcgtcga gacggtcggt gcgacgggcg cacaacgcga
     1321 ggtcacggcc tttcgcggcg aacgcccgcg ccatgccggc gccgaggccg gcgctggcac
     1381 cggtgataag gatcttctga cgggtcacgc cgacagcgta tccgtcgcgt cgagagcgac
     1441 gtttcgcagg agaagtccga gtagcggcct gcaaaacgtc actctcgcga aggagctatt
     1501 tgagtagccg cgacagtcgc cggtctgcca gcaccttgcc acccgtctgg caggtggggc
     1561 agtactgaaa tgacttgtcg gcgaacgata cttcgcggac ggtgtcgccg cacaccgggc
     1621 atggcaatcc agtgcggtcg tgaaccctta agcccgaacg cttttcgccc ttcagcgtcg
     1681 cggcctgctg gcccaccgaa cgggctaccg cgtccgtcaa cacggaaatc atcgcgtcgt
     1741 gcaacgcgga aacctgctcg acggccagct tgcccgcggt cgcgaacggc gacagcttgg
     1801 cgacgtgcag aatctcgtcg ctgtacgcgt tgccgatccc ggcgatcacc ttctggtcgg
     1861 taatcacgct cttgagccgg ccgctgttac cagccagcag cccggcgaga tcgtccggcg
     1921 acaagtccag cgcgtcgggc cccagcgcgg cgatgccggg caccttcgcg gggacgtcga
     1981 ccagccacac cgctagccgc ttctgcgtgc cggcttcggt gaggtcgaag cccgttccgt
     2041 cgccgaggtg cactcgaagc gcgatcggcc ccttgccggg cttcagcggc gcggccgcca
     2101 gcttgtccga ccagcgcagc cagccggccc gcgacagatg cgtgatcagg tgcagcccct
     2161 cgacttgaac gcccagatac ttgccccacc ggttggcgcc cgtcaccgtc tggccgtgca
     2221 gcgcggtgat cggggggtcg aacgtcttca gcaccgagaa cgccgagacg tcgacgcggc
     2281 cgatggtcaa tccgacggcg tggcgacgca ggtggtcggc taacgcttcc acctcgggca
     2341 gttcgggcat ggctccagtg tgccgtcact gacgggcgct ggtgacgtca cgcgtcacga
     2401 ggcccagcag ccagctcacg atcgacaaca ggatcgccgc ccagatcgcc gtccaccaga
     2461 agtggtcgat gtacaacccc cagcgggtgg tgtgctcggt gatccacgcg gtgatccaca
     2521 gcatgaacgc gttgatcacg atgtggatca ggccgagcgt caggatgtac agcggaatcg
     2581 acaacagctg caccagcggt ttgacgaacg cgttgaccaa accgaagatc acgccgacga
     2641 cgaagatgat cccgatcttc tgcaatgtcg tgttgccgcc gacgaagctg atcccgccca
     2701 cgagcagcgt cacaacccac agcgcaaacc cggtcaacac agcgcggagg aggaatgatc
     2761 ccatgcgctg atcctgccac ggtgagccgc gtgttgacgg ctagtgcccg gcgcttgtcg
     2821 ctcggagccg ggcaaagagg cactcactcc cgcgagaggc aagtgggaca cctgaggctt
     2881 gtgggacccc gatcgtcact tcttccacac acgtccctgc ggggggaaca cttcgaagtg
     2941 cccagctccg agcgacaagt gcgttgctag tcggcggatc gcagcaagta cgtgtccatg
     3001 atccagccgt ggcgcgcacg ggcgtcggca cgcaatgcca cgatgcgctc gccgacgtcg
     3061 cccaccgtgc cggcgatcga cagctcatgc tcggtgccca ggtacgcccc ccaccagatg
     3121 agagtgtcgg gcgggcattt tcggaacgcg cagtcgccgt cgagcatcac gaccaccgtg
     3181 ccgcgaaacc ccggatcgag tttgcgcccg gtggtgatca gcacgggctc cccaatgtcg
     3241 ttgagcggca gacgatgacg tgcggtcagt gcctgtatcg cggtgattcc ggggaccacg
     3301 tcgtacgaga tgtcgacgtg ctgggcgacg gcgtcgagga tccgcagcgt gctgtcgtac
     3361 agcgacggat cgccccacgc cagaaatgcg cccacctcac cgggcgccag cgacgcctcg
     3421 atcgcaccgg cccacaaccg cgcccgggcg gcgtgccagt cgctcaccgc tgccgtgtag
     3481 tcaccggtcg gcgctcgggc cggatcacga agttcgacaa accggtaccg ccgcgacggg
     3541 tcgatgaacc gggcgcagat gtcgcgccgc cacgccacca ggtcgctctt cgcctcgccc
     3601 ttgtccatca cgaagaacac atcggtgtcg ttcagcaccg aaacagcttg agctgtcacg
     3661 taatccgggt cgccggcgcc gatgccgatg acgtgaatgc ggcgcgtcat gtcgcaaggg
     3721 taaccgctac gatcgcacgc agtactcgaa gtatccggta cccagagtat tgactaaaac
     3781 gcaccagccg acttagcgtc gatacaagca ctgatcgacg aagggacgtc aacggtgact
     3841 accccaacaa aggccacgac acaggaagag ttctcggcac gcctgctcaa tggttcggtg
     3901 aagaagtcct acgagccggt cgtcgacatc gactgggacg cgccgctcga cccggacaag
     3961 ttctacctgc cgccgaaggt ggtgtcgctc tacggcacgc cgatgtggga cgcgatgacg
     4021 cgcgagcagc agatcgagct gtcccgccag gagttcgtca acaccctgtc ggcgggcatc
     4081 tggttcgaga acatgctgaa ccagtcactg ctgcgccaca tcctgcaccg ggatccgacc
     4141 gctaacgcga cgcactacgc gctcaccgaa ctcggcgacg agacccgcca catggtgatg
     4201 ttcggcaagg cgatcgaacg cgtcggcgcc aggccggtgc agccgcgccg ctatcagcgc
     4261 tgggtcatca actcactgcc gctggctttt cgcggcgcgt tcatgtgggt cgccgcgctg
     4321 atcggcgagg agatcttcga ctcgctgcag cgccagatga tggacgaccc cgcgttgcag
     4381 ccaatggtcc agcggctgat gcgaattcac gtcaccgaag aagcccgcca catccagttc
     4441 gcgcgcgacg gcgtgcgtaa gcgcgtcggc gaaatgccct gggtcagcag gttctgggtg
     4501 gccaacataa acggcctcgg cggcgtcttc ttccgctacc tgttcaccaa tcccgtgccg
     4561 tacaaccgcg tcggcctgga cgggcgcgaa gcccgccgcg tcgcacgaaa caacccgaac
     4621 tactacgagg ttcagcggat cgggttcgcc ccgctggccg cgttcctgca agacgtcggg
     4681 ctgatgggcc cgatcgcccg ccggctgtgg aagcgcacga agttcctgtg atgagcgctc
     4741 gcgcgaagaa ccaacagcca tgatcgacgt cgcggtgatc ggggccgacg acgacgtggt
     4801 cgcggccaac ttcgacgacg ccaccaacac ctggctgctg cggacgagct caggtggcga
     4861 gcgccgagcc cgcgtcgtca tcgacgaacg cacgctgcat ctggccgccg aatgggaccc
     4921 ggcgttcgac ccgacaggta aacgcatcgc cgtgatcggc gaccgcgccg ctcacgtcct
     4981 cccgaagctc gcgggcgcac aggccacgct gttcgactgc ccggcgacct tccagacgcg
     5041 aaacaccaag cggcgctggc tacctcgcag accacccgga cgcccgcacc tggtgacatc
     5101 gcccgtcgca catatcactt ccaaaggagt ccgcaccacc gacggcaccg aacacgtcgt
     5161 cgacgcgatc atctacgcca ccgcttccac aacgaagacg ggcctccccg acgacgcgct
     5221 cgtcggggcg aacgggctca cgatccagca agcctggcgc gacgccgcca ccgcctacta
     5281 cggcgtggca gtccacggct ttccgaatta ctttctgctg catggcccgg actcaccgat
     5341 agacaacgac caggtccgct acatcgcgga ctgcctgggc ctgatgaagc accacaacgc
     5401 cacccgcatc gaggtccgcc gcagcgcaca gcagcagtat gcccaacgag tgcggatcaa
     5461 acctcccaca atggcattcg acctcaccga cgaggcgcac gacatttacg acggaccggc
     5521 gacgctcagc ctaggcgacg acgaacacac cgtccacgca caactgaccg gtcacctcga
     5581 tccgatcgac ggcaaatatc attggcaggg aacggttctc ggcgcacaag tcgacctgcc
     5641 gaagcgcccg gtcgctatcg ccacagaggc gcgaagcgcg caagccagga tcaccgagca
     5701 gacgccgtgg ggcagctact cgatcgccgg aatcggagct ccgccttacg aactcacgta
     5761 acggccgaac cagtcgagca ctcgccgcca cgcctcggcc gctgcggccg ggttgtaccg
     5821 cgagccggtg tcgttgaaga acgcgtggtc ggcatccggg gcgacgtaca cgtcgtgaac
     5881 cagccccgca tgctgaagcg ccgcgaccgc agcgtccttc gacgcgttca cccgagcgtc
     5941 caaggcaccg tagatcgcga gcaccgccgc cttggagccg gagaagtccg gattgtcggg
     6001 cagcggtccg tagaacggca ccgcggcagc caacgacggc tcacccgacg ccagcagttg
     6061 ccacaccatc ccgccgccga agcagaaccc gacggc
//
LOCUS       DASWBF010000336         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_502843,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000336 DASWBF010000000
VERSION     DASWBF010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502843"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..764)
                     /locus_tag="VF874_12305"
     CDS             complement(402..764)
                     /locus_tag="VF874_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX9176811.1"
                     /translation="MVICPSRSRSAVAVAFSAAALAGTMLWGAAPASADPPPGCSAAD
                     RAQVMADVSSATADYLRSHPDVNAFFSNVAGQPRDAMRNQIHDYMNANPTVASDLRGI
                     RQPMKDLRNRCGPFFAKT"
     gene            complement(996..1127)
                     /locus_tag="VF874_12310"
     CDS             complement(996..1127)
                     /locus_tag="VF874_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176812.1"
                     /translation="MSVAGNVTHAAVRAPRTAVALAAAAAMVPPAVLLGSTRCVILR"
     gene            complement(1597..1854)
                     /locus_tag="VF874_12315"
     CDS             complement(1597..1854)
                     /locus_tag="VF874_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176813.1"
                     /translation="MFTGLRLLTHWIHRGHGPQGGGISVGGRHFHHYNIGIALLSAVG
                     AVGLRGSDRQRRHPAVAVAYGTANALVVDELALLLDLKDVY"
BASE COUNT          358 a          664 c          645 g          315 t
ORIGIN      
        1 gcacttcttc gacaccctcg tcatgctcgc cgaaggtcgg ccctggctgc ccgcggagac
       61 ctgactagtt actcgcgtcc gaccttctcc tgttccatgc aaaagccgca gaccgggctc
      121 gcgtcgcctc catgccggac accacctggc caatcaacgg gcacacgcca gactcatcca
      181 gccacaccgc gtgcccgatg acctcgatcg gataccggta gcccctgtac ctcgccgcaa
      241 tcgccacaac gaccaatcct cgcagccacc acacaaggtc aacaacttga cgatgccagt
      301 gaaagtgccc gccggagcaa ctcatcacgg ccgggtgttg tcgctgtgac gctgtggcgc
      361 ggcggtgatt ggcgccgcgg tctcggtcag cggcggcgag cttaagtttt cgcgaaaaac
      421 ggaccgcaac ggttacgcag gtccttcatc ggttgccgga tgcctcgaag gtctgatgcc
      481 actgtgggat tggcgttcat gtagtcgtgg atctggttgc gcatcgcgtc gcgcggctgg
      541 ccggccacgt tggagaagaa cgcattgacg tcggggtgag agcgcagata gtcggccgtc
      601 gccgacgaga cgtcggccat gacctgcgcc cgatccgccg cgctgcaccc gggcggggga
      661 tcggcggaag cgggtgcggc accccacagc atcgtgccgg ccagggccgc ggcgctgaaa
      721 gcgacagcga cagcgctgcg acttcggctg ggacagatca ccatgacgac tccttcgtcg
      781 cgttgtgtgt tgcggcgagt cttaaattcc ccacctcgca ccgaacgatt cacgttaccg
      841 cgcaggactc gggcgaacgt ggtcaatcag ctgggtggtc gctgtgacgc ggacatcacg
      901 atgcggttgc gccagcctgg cgcgtcacct ccggcgcgga ttcgacacgg gcggcgtcgg
      961 gcgtgaaacg cactggcggc gccgaagcca caatgtcacc gcaagatcac gcaacgcgtc
     1021 gagcccagca gtaccgcggg cggcaccatg gcggcagcgg ccgccagggc cacggcggtc
     1081 cgcggggcgc gcaccgcggc atgagtgacg ttgccggcca ctgacatcgc ggtcgccgtg
     1141 cacagccacg tccacaagta ccgaattccc gcacgacacc ggcctggggg gcctcgatga
     1201 ggtgccaatc tatcccttat catccgccag cgaaaaccgc tgcgccgcag aacatctcgg
     1261 gtctcgacga ccaacgctgt gccgcgtcgg ggcggcgacc gtttcagcgt atcggccttc
     1321 gggaggcggg tggtgcacaa tcttgggccg ccgccggtga ttcgcgggcc ggtcgctcaa
     1381 ggcaggccaa cgaggtgtta gcgaatcagg ccggatccgt cagcatccgg aacggtcgcc
     1441 aaatatttat cagctcagag aggatccgaa cgagcgggtg aatccggtgc gcagcacccg
     1501 ccgcgcgtgc ggccagaacg gcattccagc tgccgcggtt gcacccaggg cgatcaatgc
     1561 gattgcgacg tcgacgctct cgcggccatc gtgcgatcag tacacgtctt tgagatccag
     1621 cagcagcgcc agctcgtcga caaccagcgc attggccgtg ccgtaggcga ccgctaccgc
     1681 gggatggcgg cgctgcctgt cggagccgcg caatcccacc gcaccgaccg ccgacaggag
     1741 cgcaataccg atgttgtagt gatggaagtg gcgtccaccg acactgattc cgccgccctg
     1801 gggtccgtgc ccgcggtgaa tccagtgggt tagcagccgc agaccggtga acatcgtcgt
     1861 gaacgacgcc taggacagta ccgccgcccg ctcggtgggg ctgagccgct gaccgaactg
     1921 gtcgcgcagc cgggtccata tgtcggcgga cagtggcggc gtcatatgcg caccgagcca
     1981 gc
//
LOCUS       DASWBF010000337        24348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1219560,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000337 DASWBF010000000
VERSION     DASWBF010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24348
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219560"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1863
                     /locus_tag="VF874_12320"
     CDS             49..1863
                     /locus_tag="VF874_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEX9176814.1"
                     /translation="MSGCVALLGRLRGAKFDWVRVRVTAAALAVTLVVTGLIAAPLAT
                     ADQCSPPGVQSASSLPTNLAKAAKGPDEDKYTTESVEPLNAVNVSQLGLGTPGTLVVG
                     TLSDAPPSVCINSAGQFTGFDNELLRAVADKLGLRVQFVGTDFSGLLAQVASRRFDVG
                     SSSITTTDARRKTVGFTNGYDFGYFSLVVPPGSPITGFDKLAAGQRIGVVQGTVQEAY
                     VVDTLHLDPVKFPDYNTVYGSLKTGQIDAWVAPSQQALGTVRPGDQAVIVENTFSLDN
                     FVAWAVAKENTPLIDALNSGLDAVIADGTWARLYSEWVPRALPPGWKPGSKSAPQPTL
                     PDFAAIAASQTPRATTPPAPKSTLSQLKDSFFDWDMYKQAIPDLFKTGLPNTLILTVS
                     ASIIGVVLGMVLAVSGISRSRWLRWPARVYTDVFRGLPEVVIILLIGLGVGPVVGGLT
                     GNNPYPLGIAALGLMAAAYVGEIFRSGIQSVESGQMEAARALGFSYRRSMRLVVVPQG
                     IRRVLPALVNQFISLLKASALVYFLGLVAGQRELFQVGRDLNAQTGNLSPLVAAGLFY
                     LALTIPLTHLVNYIDTRLRRGRTVAEPEDPLAVSQEMV"
     gene            1899..2612
                     /locus_tag="VF874_12325"
     CDS             1899..2612
                     /locus_tag="VF874_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX9176815.1"
                     /translation="MSGNNIHLSFSQNAVLRGVDIDAAAGSTVAVIGPSGSGKSTLLR
                     TLNRLYEPDGGDILLDGRSVLKDNPDELRQRIGMVFQNFNLFPHRSVIDNVMLAPRTL
                     KRLSAEAARELALTQLDRVGLRHKAGVRPTTLSGGQQQRVAIARALAMAPQVMFFDEA
                     TSALDPELVKGILALIADLGAEGMTMIVVTHEMGFARSASDAVVFMDHGKVVESGPPE
                     RIFEAAETDRLQRFLSQVL"
     gene            2673..3131
                     /locus_tag="VF874_12330"
     CDS             2673..3131
                     /locus_tag="VF874_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9176816.1"
                     /translation="MVETTEPQVTELAEGLHRVLSKLVSVLRRGDTSRVATGDLTLAQ
                     LSILVTLIDRGPIRMTELAAHERVRTPTTTVAIRRLEKLGLVKRSKDQSDLRAVLVDI
                     TDKGLAVHRESLANRRAALAEMLAGLSDDDRETLAKALAPLERLAGQSAT"
     gene            complement(3139..4479)
                     /locus_tag="VF874_12335"
     CDS             complement(3139..4479)
                     /locus_tag="VF874_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HEX9176817.1"
                     /translation="MAPDPSFSPTQLAARAAYLLRGNDLGVMTSAAPLLYPHMWSWDA
                     AFVAVGLAPLSVERAVVELDTLLSAQWTNGMIPHIVFANRADAYFPGPQRWACSELAA
                     NAPRHHHTSGITQPPVHAIAVERILDHARRRGRSTRQVAESFLDRVWNDLVRWHRWLA
                     ESRDQHGRGRITLYHGWESGMDNSPRWDEAYANVVPGQVPEYQRKDNMIVMDASQRPS
                     DSEYDRYLWLLEEMKAARYDDDILPKVMSFAVEDVFVSAVFSLACDVLANIGEDYSRP
                     KADVRDLYGWAERFRSGVAETADERTGAAKDFDVRSDKWIATETVAQFAPLLCGGLPH
                     DRERALLRLLDGPRFCGHPDLRFALIPSTSPVSRDFRPREYWRGPVWPVMTWLFSWAF
                     ARRGWAERSRTLSREGLRQASDGTFAEYYEPFTGEPLGSMQQSWTAAAVLDWLG"
     gene            4530..5222
                     /locus_tag="VF874_12340"
     CDS             4530..5222
                     /locus_tag="VF874_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9176818.1"
                     /translation="MPAQTRGAQQPTALITGAGGGLGSAIADALAPTHSLLLAGRPSD
                     RLDAVAQRLGVSTLALDLSDTDTLAATADVVDELDVLVHNAGVAYPDHLAESRIGDWR
                     KTLEINVIGVVALTHALLPALRRAKGQVIFVNSGAGLNVLAGMASYSASKFAMRAYAD
                     SLRAEEPTLRVTSVHPGRIATEMQRKLREFEDGEYDEKNYLRPETVARVIADIVATPP
                     DGHVHQIIIRQR"
     gene            complement(5219..8095)
                     /gene="leuS"
                     /locus_tag="VF874_12345"
     CDS             complement(5219..8095)
                     /gene="leuS"
                     /locus_tag="VF874_12345"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031344725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9176819.1"
                     /translation="MTDSPTTAGASADADVPKFRYTGELAGEIERSWQQRWAETGIFN
                     VPNPVGSFAPTDGSTVPDDKMFVQDMFPYPSGEGLHVGHPLGYIATDVYARYFRMTGR
                     NVLHALGFDAFGLPAEQYAIQTGTHPRIRTEANIVNFRRQLGRLGFGHDSRRSFSTTD
                     VEFYKWTQWIFLQIYNAWYDRDADRARPIAELVAEFDSGARSLDDGRRWPDVSEGERA
                     DVVDSHRLVYRADSLVNWCPGLGTVLANEEVTDDGRSERGNFPVYRKRLRQWMMRITA
                     YADRLLDDLDVLDWPEKVKTMQRNWIGRSTGASALFGVTTNDGTPVDIEVFTTRPDTL
                     FGATYIVLAPEHELADVLTAADWPDDVNLRWTYGAATPADAVAAYRRSIAAKSDLERQ
                     ENKAKTGVFLGTYAANPANGECVPVFVADYVLVGYGTGAIMAVPGHDERDWEFATEFE
                     LPIVEVIAGGEVSREAYTGAGLAVNSAYLNGLDVEAAKEAITQRLEADGRGQARIEYK
                     LRDWLFARQRYWGEPFPIVYDEDGRAHPLPESELPVVLPDIPDYAPVLFDPEDADSEP
                     SPPLGTADDWVHVELDLGDGLKNYTRDTNVMPQWAGSSWYELRYTDPHNSERFCAKEN
                     EAYWMGPRPAEHGPGDPGGVDLYVGGVEHAVLHLLYSRFWHKVLYDLGHVSSSEPYRR
                     LVNQGYIQAFAYTDSRGAYVPAAEVTERDGRFCYDSPGGEVEVFQEFGKIGKSLKNSV
                     SPDEICDDYGADTLRVYEMSMGPLEASRPWATKDVVGAYRFLQRVWRLVVDEQTGDSR
                     ASEQPLDDDTLRIRHRTIAGVADDYAGLRNNTAVAKLIEYTNHLTKQAITARDAVEPL
                     VLMLAPVAPHLAEEMWRRLGHDRSLAHGPFPHADERYLVDDTVEYPVQVNGKVRGRIS
                     VVADAGADALEAAALADEKVSAFLAGATPKKVIVVPGRLVNLVV"
     gene            8254..9186
                     /locus_tag="VF874_12350"
     CDS             8254..9186
                     /locus_tag="VF874_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HEX9176820.1"
                     /translation="MNHDTRGWLAMVGGSAAGIAAIICLTSPTAVAEPIPPPVPAPVT
                     VTQTVTAAPAPGAALAAPQAVSAPAAGTPVGSAAVPGRAPVVAAPSASPVPQAGLSPA
                     APTAAAPVTPTLAPATSGTLTDYFKSKNVTLEPQRAQGFQVFNITLPIPPGWTQVPDP
                     NVPDAFAVIANRTSADLYTPNAQVVVYKLVGEFDPKEAITHGFVDSQSLAAWQTTGAS
                     LADFYGLPSSIIEGTYRQNDMTLNTSRRHVIVPTGPDKYLVSLSVTSQASSQGAPSAA
                     DATDAIVNGFRVAPVTPAAPPPAAPGLPTLPQSP"
     gene            9235..9579
                     /locus_tag="VF874_12355"
     CDS             9235..9579
                     /locus_tag="VF874_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176821.1"
                     /translation="MLVAGVLCLGAAALLVALGARALAGPAPAGGAQQVLRAVAPTQL
                     AAAVMLAAGGVVALSRQSSIGLLVLCIAGAMATVAAGAWQGARFAQATRQQDACQRAG
                     GLRCAACTLSCR"
     gene            complement(9612..10214)
                     /locus_tag="VF874_12360"
     CDS             complement(9612..10214)
                     /locus_tag="VF874_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX9176822.1"
                     /translation="MAHSEDPEDYAPTAHRVRSGTLLLANTDLLEPTFRRSVIYVVEH
                     NDGGTLGVVLNRPSETAVYNVLPQWARLTAKPKTMFIGGPVKRDAALCLATLRAGVTC
                     DGVPGLRHVQGRMVMVDLDADPDSIAPVVEGVRIFAGYSGWTIGQLEGEIERDDWIVL
                     SALPSDVLVEPRVDLWARALRRQPWPLSLLATHPIDISRN"
     gene            10331..11620
                     /locus_tag="VF874_12365"
     CDS             10331..11620
                     /locus_tag="VF874_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9176823.1"
                     /translation="MVDVRAPVAVWRSVRGLPEFGRLLGVRIASQFGDGLFLAGLVGA
                     TLFDPDRAATPGDVAWSYAVLFLPYSFLGPFAGALLDRWDRRYVLVGANAARLVLITA
                     VGALLAFGADLWAIRAGALLVNGVARFVTSGLSAAMPHVVPREQVVTMNAVATAVGAV
                     ASFIGAGFMLLPRWLFGANDTGSATVIFIVVIPVLIALLLALRFPPHSLGPDDTALTV
                     HGSAVYAAATGWLHGLRTVVGTPGVAATLAGLAAHRIAFGINTMLMLVIVRHTDAQVL
                     AGLGLAVLFAAATGTGSFLANVLTPAIVRRVGRFAAANAALATAAGIQVFGAGLVLSV
                     IVVCGFLLGAAGQVVKLCADSAMQIDVDDARRGHVFAVQDSLFWLSFVGAAAASAAVI
                     PADGHSPALALTGAGTYLVGLVVHTVVGRRGPIAHGR"
     gene            11631..12404
                     /locus_tag="VF874_12370"
     CDS             11631..12404
                     /locus_tag="VF874_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015626305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HEX9176824.1"
                     /translation="MADAAPIVDDLRAQSDELDALVAPLPENRWREPTPAQGWTIAHQ
                     ISHLLWTDRVALTSVTDEPGFDELLSAAAADPTGFVDAAAEEESRRSADDLLADWRAT
                     RRRLHDALLEVPPTRKLVWFGPPMSAASMATARLMETWAHGLDVADALGVQRGAGERL
                     RSIAHLGVRTRDYAFAVHGLPVPAEPFRVELRAPDGSAWAWGPEDAAQRITGSALDFC
                     YLVTQRRPLSALDIEAVGDDAKRWLAIAQAFAGPPGPGR"
     gene            complement(12408..12788)
                     /locus_tag="VF874_12375"
     CDS             complement(12408..12788)
                     /locus_tag="VF874_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9176825.1"
                     /translation="MRDNGFVLDASAMVDLLVGRRSFRQRLNEATVHVPAHFDAEVLS
                     ALGRLWRAELLSSDEVAFALAALMSAPFKRHTLEDIVIGAWGRRDNLRLVDALYVEIA
                     ERIGVPLLTTDVRLASAYTGAQLL"
     gene            complement(12785..13003)
                     /locus_tag="VF874_12380"
     CDS             complement(12785..13003)
                     /locus_tag="VF874_12380"
                     /inference="COORDINATES: protein motif:HMM:NF019009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HEX9176826.1"
                     /translation="MARLNVYVPDELAEQARTHGLNVSALTQAAIAAELARTSMQGWL
                     ATLPAVSQPIERDAITAALDAARDEFGR"
     gene            complement(13033..13542)
                     /locus_tag="VF874_12385"
     CDS             complement(13033..13542)
                     /locus_tag="VF874_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEX9176827.1"
                     /translation="MEYCFGAGDGSATMWIGTPDTDLDGDGSLDALRLDFDGDGSVDD
                     AIGDLDGDGLADHVALDLDDDGTAESYFTDDGSGTWAVTVDRAGQVRWYGLDGIEHTG
                     GPLVDFDADGQADDQVIDTDRNGLADRVLCDGPSGALGYVDSDGDGRWDVELADTNGD
                     GAADTSKQL"
     gene            complement(13562..14998)
                     /locus_tag="VF874_12390"
     CDS             complement(13562..14998)
                     /locus_tag="VF874_12390"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX9176828.1"
                     /translation="MPETASDAELLAGAAIALNQHTDILRALGAVFAGAGHELYLVGG
                     SVRDAILGRLGADLDFATDAEPEQVQQMLRQWADALWDTGIQFGTVGAAKDAHRLEIT
                     TYRSDTYDQVSRNPQVRFGRSIDEDLGRRDFTANAMAVRITADGPAEFVDPLGGLGAI
                     RQRVLDTPAPPEVSFGDDPLRMLRAARFVSQLGFSVVQRVRAAIAAMAPQLGRITVER
                     VAAELDKLMLGNDPVAGVELMVDTGIGDVVLPEIGAMRLTIDEHHQHKDVYRHSLTVL
                     RQAVALEDEGPDLVLRLAALLHDIGKPDTRKHEPDGGVSFHQHEVVGAKMARKRMRTL
                     KYSKQMIDDVSQLVYLHLRFHGYGEGTWTDSAVRRYVTDAGPLLLKLHKLVRADCTTR
                     NRRRAERLQANYDDLEKRIAELAAKEDLQRVRPDLDGNEIMELLDIPAGPQVGEAWRY
                     LKELRMERGPMGKEEATAELLAWWNARQ"
     gene            15087..15815
                     /locus_tag="VF874_12395"
     CDS             15087..15815
                     /locus_tag="VF874_12395"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9176829.1"
                     /translation="MSDGERARPRRRRGRRRGRRAAAPADTAPKQTPEQAETAPASPG
                     RRSPDRLRTVHETSAGGLVVDGLDDPQGSHVAALIGRIDRRGRMLWSLPKGHIELGET
                     AEQTAIREVAEETGIQGDVVACLGSIDYWFVTEGRRVHKTVHHYLLRFLGGELSDHDL
                     EVSEVAWVPLDQLPSRLAYADERRLAEVADELIQTLHTHGPAALPPLPYSAPWRRRQT
                     HSRTRRRRSDETESGRTNGHRHQQ"
     gene            15812..18151
                     /locus_tag="VF874_12400"
     CDS             15812..18151
                     /locus_tag="VF874_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEX9176830.1"
                     /translation="MTHRRRRASLRFTAATTLRVIAAAVLVAFPLLATVIPRAVAGEP
                     GDVRFLEVRIDRVTPEIVTTTGDATVTVTGTVTNVGDRSVRDIVVRLEHAGEVTSPQV
                     LRTSLDGATDQYQPVSDFVTVAPELRRGQSGAFTLSYPVRSTSAPSLGINEPGVYPLL
                     VNVNGTPDYGEPARLDDARFLLPVLGVPADPSVESTDTLTSVVAPDTAKPVLITMLWP
                     LADRPRLAPGAPGGVTPVRLVDDELAVSLAPGGRLDTLLSAADFATGPSIDPDGRVNG
                     ALCLAVDPDLLVTVNAMTAGYVVADNPDDRNSPTHPGAGQAAAAAWLDRLRTLARRMC
                     VAPTPYAQADLDALHRVGDPGLATIATNGAGDIVDQILGVTSTRGATLLGDASLTEGA
                     ADLLNAQGPTVAIAAANCTAQDEADGKLQPANVNPHRLSDQLVTAPFDPTVGAALAGA
                     GRTPASPSYLDYSLVVPYKHDSATARRQDAISAMLWLGLNPDAAPRSQILMPPLFWDL
                     QGADAQALLTALATTIRAGLAVPRPLPAVIGDAEAAAVPSSQAPGKAAQRGRVDDAVA
                     SDMREQVGRLWGLTAALTTDPRTGLTGTQYTAPLREDILRALSQAFPPDGRNEQAQQR
                     MAAVNVTVDDMFGAVTIVNPGGSYTLATEHSPLPLALRNDLPVPIKVRLQVDAPPGMT
                     VTDIGVQELPPGYLPLRVPIEVHFTQRVAVDVALRTPDGLLLGEPVRLSVHSNAYGKV
                     LFAITMTAAAVLVVLAGRRLWHRFRGQPDPADLDRPEPE"
     gene            18148..21654
                     /gene="murJ"
                     /locus_tag="VF874_12405"
     CDS             18148..21654
                     /gene="murJ"
                     /locus_tag="VF874_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX9176831.1"
                     /translation="MTAAGRRPELTDAAVVSRSWGMAFATLVSRITGFTRIVLLAAIL
                     GAALSSAFSVANQLPNLVAALVLEATFTAIFVPVLARAERDDADGGATFVRRLVTLAT
                     TLLLITTALSVVAAPLLVRLMLGSDPQVDKQLTVAFAYLLLPQVLFYGLSSVFMAILN
                     TRNVFGPPAWAPVVNNIVAIVTLGVYVLVPGELSVEPGNTKLLVLGIGTTLGVVAQSV
                     VLLIAIKRERISLRPLWGVDDRLKQFGTMAGAMVLYVLISQLGLVVGNQIASWAAASG
                     PAIYNYTWLVLMLPFGMIGVTVLTVVMPRLSRNAAADDIPAVLDDLALATRLTMVTLI
                     PTVAFMTVGGAAIGSALFAYGKFGSVDASYLGSAIALSAFALIPYAMVLLQLRVFYAR
                     QQPWTPIAIIVVITTVKVAASLGVQQLTEHHVISNPEMVAGYLGLANGLGFLAGAIAG
                     YLLLRPALNPPSGYMFSADVVRTVLVTIAASLLAAMVAYIADQLLHFQNLTESGGGAG
                     SLLRLLLLGSIMLPIVAAVMLAAGVAEAHAALAAVRRRLGRAAVKSSPASSPVSDRPR
                     SDTSVTYPEQRLSFAQRPRADVQEGMAVTNETAGSPDATARLTRPSADDFQPDVAESP
                     ADTRATSPADTRATTEVPMRGRSPGDFAGDPTRESMGIAAPRDTADSSRSDQEGHLIP
                     GASIADGRYRLLVFHGGPEHLQFWQALDTALNRQVALTFVDPEGQLPVDRVQDILART
                     LRLSRITMPGIARVLDVARARTGGLVVSEWIRGGSLQEVADTLPSPVGGARAMQSLAT
                     AADAAHRAGVALSIDHPSRVRVSIEGDVALAFPATLPGANPDADLRGIGAALYALLVN
                     RWPLPESGVPSGFAPADSDPAGNPVEPKAVNRDIPFQISAAAVRSIQVNGGITSAATL
                     SHLLQQATAEADRTDLITPVEEPLPASPSRFRGAGFRGWGDLDEEERTRRRKGLLIGL
                     GVVGAVVVVATLLAAALFNGVGDDYSSTENQLGLNPTSGLPAPAGNGGDVKPVGAAVF
                     SPDGGADDPDNAGRAVDGNTSTAWATDVYTNSVPFPKFKNGVGLMLELPKSTTLSAVD
                     VNVSSTGTKIQIRSATTASPSSLEDTTALTEPTSVQPGPNRIPVTMSSPTSYVLVWIS
                     TLGTTDGESQADIYEITLEGTS"
     gene            21846..22424
                     /gene="sigM"
                     /locus_tag="VF874_12410"
     CDS             21846..22424
                     /gene="sigM"
                     /locus_tag="VF874_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HEX9176832.1"
                     /translation="MGRFGGAQQPQRSDAELLAAHVGGDRYAFEELFLRHHRQLYRLA
                     RLTSRTSEDAADALQDAMLSAHRSAASFRNDAAVSSWLYRIVVNACLDRLRRNKIHLT
                     VCIEDDAYPVSDSTSRSDTAVVVQRALMRLPVDQRAAVVAVDMQGYSVADAARLLGVP
                     QGTVKSRCARARARLALMLRYFDQDAESGAAG"
     gene            22434..23042
                     /locus_tag="VF874_12415"
     CDS             22434..23042
                     /locus_tag="VF874_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176833.1"
                     /translation="MDTKRGSSERQSSSDGPAIERVRAELAGLSDDAASAPDVPRPVA
                     ERVVSALRTAPPVRRTPAVRISAAVGVAAALVAAGIGTVMLQRSGSSVPPTESSAKHL
                     PEPAAATVPLSGDEIVALLSRPPDLGALGDARRRSSCLSGLGYPGPDAVLGGQQVDIN
                     GAPAVLLVLPGDTPDTVAALAVRPNCSSIDTGLLADTQIRRP"
     gene            23112..24086
                     /gene="trxB"
                     /locus_tag="VF874_12420"
     CDS             23112..24086
                     /gene="trxB"
                     /locus_tag="VF874_12420"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9176834.1"
                     /translation="MTSSPTVHDVIIIGSGPAGYTAAIYAARAQLAPLVFEGSQFGGA
                     LMTTTDVENYPGFRAGITGPELMDEMREQALRFGADLRMEDVDAVSLQGPVKTVAVGD
                     ETHRARAVILAMGAAPRYLGVPGEQNLLGRGVSSCATCDGFFFRDQDIAVIGGGDSAM
                     EEATFLTRFARSVTIVHRREEFRASKIMLKRAEDNDKIRFLTNTVVTEVLGTDSVTGL
                     RLHNTLTGEDSTLGVTGVFVAVGHDPRSELVRDQLDVDRDGYVLVRGRSTYTSIDGVF
                     AAGDLVDRTYRQAVTAAGTGCSASIDAERWLAETAHVDTDDNLIGAPL"
     gene            24083..>24348
                     /gene="trxA"
                     /locus_tag="VF874_12425"
     CDS             24083..>24348
                     /gene="trxA"
                     /locus_tag="VF874_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9176835.1"
                     /translation="MSDTTATIAVTDDSFAQDVLANERPVLVDFWATWCSPCRMVAPV
                     LEEIAAEKAGALTVAKIDIDANPGTARDFQVISIPTLILFKGGEP"
BASE COUNT         3779 a         8450 c         8103 g         4016 t
ORIGIN      
        1 gggcaagatc atgcggcgcg agttgcggga gcgcgagcga gcgaagggat gagtggctgc
       61 gtcgctcttc tcggtcgtct gcgcggggct aagttcgact gggtccgggt gcgggtaacg
      121 gccgccgcgc tggccgtgac cctggtggtg accggcctca ttgccgctcc cttggcgaca
      181 gcggaccagt gctcgccgcc cggcgtgcag tcggcgagct cgctaccgac caacctcgcc
      241 aaggccgcca aagggcccga cgaggacaag tacacaaccg aatccgtcga gccgctcaat
      301 gcggtcaacg tgagccaact cggcctcggc acacccggca cgctcgtcgt cggcacgctg
      361 tcggatgcgc cgccgagcgt gtgcatcaat tcggcgggcc agttcaccgg tttcgacaac
      421 gagctgttac gggccgtcgc cgacaagctc ggacttcggg tgcagttcgt cggaaccgac
      481 ttctcgggtc tgctggcgca ggtcgcgtcg cgtcgtttcg acgtcggctc gtcgtcgatc
      541 accaccaccg atgcacgcag gaagacggtc gggttcacca acggctacga cttcggctac
      601 ttctcgctcg tcgtcccgcc tggctcgccg atcaccggtt tcgacaagct ggcggcgggc
      661 caacgcatcg gcgtggtgca gggcaccgtg caagaggctt acgtcgtcga caccctgcac
      721 ctggacccgg tgaagtttcc cgactacaac accgtgtacg gcagcctgaa gacggggcag
      781 atcgacgcat gggtggcgcc gtcgcaacag gcactcggca cggtgcggcc cggcgatcag
      841 gccgtgatcg tcgaaaacac cttcagcttg gacaatttcg tggcctgggc ggtcgcgaag
      901 gagaacacgc cgctgatcga cgcgctcaac tccgggctgg acgcggtcat cgccgacggc
      961 acgtgggcga ggctgtattc cgaatgggtg ccgcgcgcgc tgccgcccgg ctggaagccc
     1021 ggctcgaagt ccgcgccgca gccgacgctg cccgacttcg ctgcgatcgc ggccagccaa
     1081 accccgcgcg ccaccacccc gccagcgccg aaatccacgc tgtctcaact caaagactca
     1141 ttcttcgatt gggatatgta caagcaggcg attccagacc tgttcaagac cggcctgccg
     1201 aacacgctga tcctgacggt cagcgccagc atcatcggcg tggtgctggg catggtgctg
     1261 gcagtctcgg gcatatcccg gtcgcggtgg ctgcgctggc cggcgcgggt gtacaccgac
     1321 gtcttccgcg gcctgccgga agtggtgatc atcttgctga tcggcctggg cgtcgggccg
     1381 gtggtcggcg gcttgaccgg caacaacccc tatccgctgg gcatcgcggc gctgggattg
     1441 atggcggcgg cctacgtcgg cgagatcttc cggtccggca tccaaagcgt cgagtccggg
     1501 cagatggaag cggcccgcgc gctggggttc agctaccgcc ggtcgatgcg gttagtggtt
     1561 gtgccgcaag gtattcggag agtcctgccg gcattggtca accagttcat ctcattactg
     1621 aaggcatcgg cgctggttta tttcctgggc ctggtcgccg ggcaacgcga gctctttcag
     1681 gtcggccgcg acctcaacgc acagaccggc aacctgtcgc ctttggtggc ggccggcctg
     1741 ttctatctgg cgttgacgat tccgctgacc cacctggtga attacatcga cacccggctg
     1801 cgccgagggc gcaccgtcgc agagccggaa gacccactgg ccgtgtcgca ggagatggtg
     1861 tagctgtgac ggacacggcg cgcttcgaac cggtatcttt gtcgggcaac aacattcatt
     1921 tgtcattcag tcagaacgcg gtgttgcgcg gagtcgacat cgacgcggcg gcgggcagca
     1981 ccgtggccgt gatcggaccg tccggttcgg gtaagtcgac gcttctgcgt accctcaacc
     2041 ggctttacga acctgacggc ggcgacatcc tgctcgacgg ccggtcggtg ctgaaggaca
     2101 accccgacga actgcggcag cgaatcggca tggtgttcca gaacttcaat ctgttcccgc
     2161 accgaagcgt gatcgacaac gtgatgctag cgccgcgcac gctcaagcgg ttgtcggcgg
     2221 aggccgcccg cgagctggcg ctgacgcagt tggaccgagt tggattgcgg cacaaggcgg
     2281 gtgtccgtcc aacaacgctg tcgggagggc agcagcagcg ggtcgccatc gcacgggcac
     2341 tcgcgatggc accgcaggtg atgttcttcg acgaagcaac gtcggcgctc gatccggaac
     2401 tggtgaaggg cattctcgcg ttgatcgcgg acttaggcgc cgaaggaatg acgatgatcg
     2461 tcgtcaccca tgagatgggc ttcgcccggt cggcgtcaga cgccgtcgtg ttcatggatc
     2521 acggaaaagt ggtggaatcc ggcccaccgg agcggatttt cgaagccgcc gaaaccgacc
     2581 gcttgcagcg attcctgtcg caggtgttgt gattttcggc gaaatcgacg gattcggtcc
     2641 tggcaggccc cgacaggtta gactggcgaa ttatggtgga gacgaccgag ccgcaggtca
     2701 ccgagctggc cgaggggctg catcgggtgc tctcgaagct tgtctccgtg ctgcgccgcg
     2761 gcgacaccag ccgtgtcgcc accggcgacc tgacgctggc gcagctctcg atcctggtga
     2821 cgctgatcga ccgcggcccg atccggatga cggagttggc cgcgcacgag cgcgttcgca
     2881 cgcccaccac cacggtcgcg atccgacggc tggagaaact cgggctggtg aagcggtcca
     2941 aggaccagtc cgatttgcgc gcggtgctgg tcgacatcac cgacaagggc ctggcggtac
     3001 accgggagtc gcttgcgaac cggcgtgcgg ctctggccga gatgctggcc gggctgtccg
     3061 acgacgaccg cgaaacgctg gccaaggcgc tggcgccgct ggaacggctc gccgggcagt
     3121 cggccaccta accgggcact agcccagcca gtccagtacc gccgccgccg tccacgactg
     3181 ctgcatactg cccagcggtt caccggtgaa cggctcgtag tattcggcga atgtgccgtc
     3241 gctggcctgg cgcaacccct cccggctcag cgtgcgcgaa cgttccgccc aaccgcgccg
     3301 cgcaaacgcc caggagaaca gccacgtcat caccggccac accggcccgc gccagtactc
     3361 ccgcggccgg aagtcccgcg acaccggtga cgtcgatgga atcagcgcga accgcaagtc
     3421 cggatgcccg cagaacctcg gtccatccag tagccgcagc agggcgcgtt cgcggtcgtg
     3481 cggcaagccc ccgcacagca gcggagcgaa ctgggcaacc gtctcggtgg cgatccactt
     3541 gtcgcttcgg acatcaaaat cttttgccgc accggttctt tcgtcggctg tctcggccac
     3601 accggagcgg aacctctcgg cccatccgta gagatcgcgc acgtcggctt tcgggcggct
     3661 gtagtcttcg ccgatgttgg ccagcacgtc gcacgccagc gagaacacgg ctgagacgaa
     3721 gacgtcctca accgcaaaac tcatcacctt cggcaagata tcgtcgtcgt accgggccgc
     3781 cttcatctcc tcgagcagcc acaaatagcg gtcgtattcg ctgtcgctgg gtcgctggga
     3841 ggcgtccatc acgatcatgt tgtctttgcg ctggtattcg gggacctgac cgggaacgac
     3901 gttggcgtag gcctcatccc agcgcggcga gttgtccatg ccggattccc agccgtggta
     3961 gagcgtgatg cggccgcggc cgtgctggtc gcgcgactct gccagccaac gatgccagcg
     4021 aacgagatcg ttccaaaccc gatccaggaa cgactccgcc acctggcggg tcgaccggcc
     4081 gcgtctacgg gcgtggtcga ggattcgctc aaccgcgatg gcgtgcaccg gcggctgagt
     4141 gatgcccgac gtgtgatggt gccgcggcgc gttggccgca agctcggagc acgcccaccg
     4201 ttgaggtccg gggaagtacg cgtcggcccg gttggcgaac acgatgtgcg ggatcatccc
     4261 attggtccac tgcgccgaga gcagcgtgtc gagctcgacg accgcgcgct cgacactcag
     4321 tggcgccagg ccgaccgcca cgaacgcagc gtcccagctc cacatgtgcg ggtacaacaa
     4381 cggcgcggcg ctcgtcatca cacccaggtc gttgccgcgc agcaggtacg cagcacgagc
     4441 ggcgagctgc gtcggcgaga agctggggtc cggtgccatc ggttctatga tgcggccgcg
     4501 gcgctgcgtt cgcccgctaa tgtggcgaca tgccggcgca gacgcgagga gcacaacaac
     4561 ccaccgcgct gatcaccggg gccggcggcg gcctgggttc cgccatcgcc gatgcgctgg
     4621 cgccgacgca ctcgctgcta cttgccggcc gcccgtccga ccgcctcgac gccgtcgcac
     4681 agcgactcgg cgtgagcaca ctggcactcg acttgagtga caccgacacg ctggccgcaa
     4741 ccgctgacgt cgtcgacgag ctcgacgtgc tggtgcacaa cgccggggtc gcatacccgg
     4801 atcaccttgc cgagtcgcgg atcggggact ggcgaaagac cttagagatc aacgttattg
     4861 gcgtggtcgc gttgacgcac gcgctgctgc ccgcgcttcg gcgcgcgaaa ggccaagtaa
     4921 tctttgtcaa ttcgggtgcg ggactgaatg tgctggccgg catggcctcg tattcggcca
     4981 gtaagttcgc aatgcgggcg tacgccgatt cgctgcgcgc ggaggagccg accttgcggg
     5041 tcacctcggt gcaccccggt cgcatcgcca cggagatgca gcgcaagctc cgggagttcg
     5101 aggacggcga gtacgacgag aagaactacc tgcggcctga gacggtggcc cgcgtgatcg
     5161 ccgacatcgt cgccacaccg ccggacggcc atgtgcacca gatcataatc cggcagcgct
     5221 agacgaccag gttcaccagc cggcccggaa cgacgatcac cttcttcggc gtcgcaccgg
     5281 caagaaacgc cgacaccttc tcgtcggcca gcgcagcggc ctcgagcgcg tcggcgcccg
     5341 cgtcggcgac caccgaaatg cggccccgca ctttgccgtt cacctgcacc gggtactcga
     5401 cggtatcgtc taccagatac cgctcgtcgg cgtgcgggaa cggcccgtgc gccaacgagc
     5461 ggtcgtggcc cagccgccgc cacatctcct cggccaggtg cggggccacc ggcgcgagca
     5521 tcaacaccag cggctccacc gcgtcgcgtg ccgtgatcgc ctgcttcgtc aggtgattcg
     5581 tgtactcgat cagcttggcc accgcggtgt tgttgcgaag ccccgcgtag tcgtctgcga
     5641 ccccggcgat agtgcggtgc cggatccgca gcgtgtcgtc gtccagcggc tgttcggacg
     5701 cgcgggagtc gccggtctgc tcgtcgacaa ccagccgcca cacccgttga aggaagcggt
     5761 aggcaccgac cacatccttt gtcgcccagg gccgcgacgc ctccagcgga cccatcgaca
     5821 tctcgtacac ccgcagcgtg tcggcgccgt agtcgtcgca aatctcgtcg ggtgacaccg
     5881 agttcttcag gctcttgccg atcttgccga actcctggaa cacctcgacc tccccgcccg
     5941 ggctgtcgta gcagaatcgg ccgtcgcgct cggtgacctc ggccgccggc acgtaggcgc
     6001 cgcgggagtc ggtgtaggcg aacgcctgaa tgtagccctg gttgaccagc cggcggtacg
     6061 gctcgctcga gctgacgtgc cccaggtcgt agagcacctt gtgccagaac cgggaataca
     6121 gcaggtgcag caccgcatgt tcgacgccgc cgacgtacag gtcgactccg ccggggtcgc
     6181 cgggcccgtg ctcggccggc cgcggtccca tccagtacgc ctcgttctcc ttggcgcaga
     6241 accgttccga gttgtgcggg tcggtgtagc gcagctcgta ccatgagctg cccgcccact
     6301 gcggcatcac attggtgtcg cgggtgtagt tcttcagccc gtcgccgagg tccagttcga
     6361 cgtgcaccca gtcgtcggcc gtgcccagtg gcggcgacgg ctcgctgtca gcgtcctcgg
     6421 ggtcgaacag taccggtgcg tagtcgggga tgtcgggcaa caccaccggc agctcggact
     6481 cgggcagcgg atgggcccgg ccgtcctcgt cgtacacgat cggaaacggc tcgccccaat
     6541 agcgctgccg ggcgaacaac cagtcccgca gcttgtattc gatgcgagcc tggccgcggc
     6601 cgtcggcctc cagccgttgc gtgatcgcct ctttagccgc ctccacatcc aacccgttga
     6661 ggtaggccga gttcaccgcc aggccggcac cggtgtacgc ctcacgggaa acctcgcccc
     6721 cagcaatgac ttccacgatc ggcagctcga attcggtggc gaattcccag tctcgctcgt
     6781 cgtgccccgg caccgccatg atcgccccgg tgccgtatcc gacgagaacg taatcggcga
     6841 cgaagaccgg gacgcactca ccgttggccg ggttggccgc gtatgtgcca aggaacacac
     6901 cggttttcgc cttgttctcc tgacgttcca gatcggactt ggccgcgatg gagcgccggt
     6961 aggccgccac cgcatcggca ggcgtcgccg ccccgtacgt ccagcgcagg ttgacgtcgt
     7021 cgggccagtc ggcggccgtg agcacgtcgg ctagttcatg ctcgggtgcc agtacgatgt
     7081 acgtggcacc gaacagcgtg tccggcctcg tggtgaacac ctcgatgtcc accggcgtac
     7141 cgtcgttcgt ggtgacgccg aacaacgccg acgctccggt ggagcggccg atccagttgc
     7201 gctgcatggt cttgaccttc tcgggccagt ccagcacgtc gagatcgtcg agcaatcggt
     7261 ccgcgtaagc tgtgattcgc atcatccact gtcgcaaccg tttccggtag acggggaagt
     7321 tcccgcgctc gctgcgcccg tcgtcggtga cctcctcgtt ggccagcacc gtgcccaggc
     7381 cggggcacca attcaccagc gaatcggcgc gatacaccaa ccggtgagag tcaacgacgt
     7441 cggcacgctc gccctcggat acgtcaggcc agcgacggcc gtcgtccaat gaccttgcac
     7501 cggagtcgaa ctcggcgacc aattccgcga tcggccgcgc cctgtccgcg tctctgtcgt
     7561 accacgcgtt gtagatctgc aagaagatcc actgcgtcca cttgtagaac tcgacgtccg
     7621 tggtggagaa actgcgccga gagtcgtggc cgaaaccgag ccggcccagc tgacgacgga
     7681 agttgacgat gttggcttcg gtgcggatgc gcggatgggt gccggtctgg atcgcgtact
     7741 gctcggcggg caacccgaac gcgtcgaaac ccaacgcgtg caacacattg cggccggtca
     7801 tccggaagta gcgggcgtag acgtcggtcg cgatgtagcc cagcggatgg ccgacgtgca
     7861 ggccctcccc ggatgggtat gggaacatgt cttgcacgaa catcttgtcg tctggcaccg
     7921 tcgagccatc tgtcggcgcg aatgacccga cagggttcgg cacgttgaag atgcccgtct
     7981 ccgcccagcg ctgctgccag gaccgctcga tctcgccggc aagctccccg gtgtagcgga
     8041 acttcggcac gtcggcgtcg gcgcttgcgc ccgcggtggt cggcgagtcg gtcactccca
     8101 caggttagtc agcgtgacgc agaggtcatt cggcccgtgg caggtctcgg ttgcgttgcg
     8161 gaccggtcag aactcggttc cagcactggt ggtaccagag tgacagttgt tacggtcgcg
     8221 aggttcgctt gcgcggaagg aggacacggc gggatgaacc atgacacccg gggctggctc
     8281 gcgatggtgg gtggatcggc ggcaggcatc gccgcgatca tctgcttgac cagtcccacc
     8341 gccgtcgccg agccgatccc gcctcctgtg ccggcgcccg tcacggtcac ccagacagtc
     8401 accgccgcgc cagcgccggg ggcagcgctg gcagcgccgc aggccgtgag cgcacccgcc
     8461 gccggtacgc ccgtcgggtc cgcggccgtc cccggcagag cacccgtcgt cgcagctccc
     8521 agcgcctcgc cggtaccaca ggcgggactg tccccggcgg cgccgacggc ggcggcaccc
     8581 gtcacaccca cactcgcccc ggccacgtcg ggcacgctca ccgactactt caagagcaag
     8641 aacgtcacgc tcgaaccgca acgcgcccaa ggttttcagg tcttcaacat cacgttgccg
     8701 ataccgccgg gctggacgca ggtacccgac ccgaacgtcc cggacgcgtt cgcggtgatc
     8761 gccaaccgca ccagcgcaga cctctacacg ccgaacgctc aggtcgtcgt ctacaagctc
     8821 gtcggcgaat tcgaccccaa ggaggccatc acccacgggt tcgtcgacag ccagtcgctg
     8881 gccgcgtggc agaccaccgg cgcctcgctc gctgacttct acgggcttcc gtcgtcgatc
     8941 atcgagggca cctaccggca gaacgacatg acactgaaca cgtcgcggcg acacgtgatc
     9001 gtgccgaccg gtcccgacaa gtacctggtg tcgttgtcgg tcacctccca ggccagcagt
     9061 cagggagcgc ccagcgccgc cgatgccacc gacgcgatcg tcaacgggtt ccgggtcgcg
     9121 cccgtcacgc cggctgcgcc accacccgcg gccccgggcc tgccgacact gcctcaatcg
     9181 ccgtaagcgc gtccccgatc tgcgggcgca ccgggcctct cgtattgtgt ctccatgctc
     9241 gtggcggggg tactgtgcct gggtgccgcc gcgctcctgg tggcgcttgg cgcgcgggcc
     9301 ctggccggcc cagctccggc cggcggagcc caacaggtgc tacgggcggt ggcaccgacc
     9361 cagctcgcgg ccgcggtgat gctggctgcc ggcggcgtgg tcgcgctgtc gcgccaatcc
     9421 tcgatcgggc tgctggtgtt gtgcatcgcc ggcgcaatgg caacggtcgc cgcgggcgcg
     9481 tggcagggcg cccgcttcgc gcaggcgacg cggcagcagg atgcgtgtca gcgagccggc
     9541 ggtctgcgct gcgcggcgtg caccctgtcg tgtcggtagc gggacgtatt gccgctgaag
     9601 gtttttgggc ctcagttgcg gctgatgtcg attgggtgcg tcgccagcag cgacaacggc
     9661 cacggctgac ggcgcagcgc gcgcgcccac agatccaccc tcggctccac aagcacgtca
     9721 gaaggcaacg cggacaacac aatccagtcg tcccgctcga tctcgccttc caactggccg
     9781 atggtccagc ctgagtaccc ggcgaagatg cgcacgcctt cgaccacggg tgcgatcgag
     9841 tcggggtcgg cgtccaggtc gaccatcacc atgcggccct gcacatgtcg tagacccggg
     9901 acaccgtcac aggtcacccc cgcccgcagc gtcgccaggc acagcgccgc gtcgcgtttc
     9961 accgggccgc cgatgaacat cgtcttcggc ttggcggtca gtctcgccca ctgcggaagc
    10021 acgttgtaga ccgcggtttc gctcggccgg ttcagcacca ccccgagggt gccgccgtcg
    10081 ttgtgctcga cgacgtagat cacactgcgc cggaacgtcg gctccaacag atcggtgttg
    10141 gccaacagca gagtgccgga ccgcacccgg tgcgcggtgg gtgcgtagtc ctccggatct
    10201 tctgagtggg ccacgtcacc atcatgtcac cagcacagcg caattggggc gatcagcgaa
    10261 cgacgatccg aatctatgta ctgtaggttc ctcgtcgctc tccggtggcg ggtggcagga
    10321 agtgacttcg gtggttgacg tgcgcgcacc ggtcgccgtg tggcgatccg tacgtgggtt
    10381 gcccgagttc ggccggctgc tcggggtgcg gatcgccagc cagttcggcg acggtttgtt
    10441 ccttgccggg ttggtgggcg caaccctgtt cgatcccgat cgggccgcaa caccagggga
    10501 cgtcgcgtgg tcgtacgcgg tgctgttcct gccttactca tttctcgggc cgttcgccgg
    10561 agcgctgctg gaccggtggg accgccggta tgtgctggtc ggcgcgaacg cggcgcggct
    10621 ggtattgatc accgccgtcg gagcgctgtt ggcgttcggc gcagacttgt gggcgatccg
    10681 tgccggggcg ctgctggtca acggtgtcgc ccggttcgtc acatccgggc tttcagcggc
    10741 gatgccacac gtggtgcccc gcgaacaggt cgtcacgatg aacgcggtcg ccaccgcggt
    10801 cggcgcggtt gcaagcttca tcggcgccgg tttcatgctg ttgccccggt ggttgttcgg
    10861 cgcgaacgac accggttctg ccacagtcat tttcatcgtc gtcattcccg tactgatcgc
    10921 cttgctgctg gcattgcggt tcccgcccca cagcctgggc cctgacgaca ctgcgctcac
    10981 ggtccacggt tcggcggtgt atgcggccgc aaccggctgg ctgcatgggc tgcgaactgt
    11041 cgtcgggacg cccggcgtcg cggccacgct ggccggactc gcggcacatc gcatcgcgtt
    11101 cggcatcaac accatgctga tgctggtgat cgtgcggcac accgacgcgc aggttctcgc
    11161 gggcctgggc ctggcggtcc tcttcgcagc ggccaccggc accggttctt tcctcgccaa
    11221 cgtgctgacc ccggcgatcg tgcggcgcgt cggcagattc gccgccgcca acgcggccct
    11281 cgccaccgcg gccggcatcc aggtgttcgg cgccgggttg gtgctgtcgg tgatcgtggt
    11341 gtgcggattt ctgctgggtg ccgcaggaca ggtggtcaag ctgtgcgcgg acagcgcgat
    11401 gcaaatcgac gtcgacgacg cgcgccgggg gcacgtgttc gcggtacagg actcgctgtt
    11461 ctggctgtcc ttcgtcggcg cggccgctgc cagcgcggcc gtgattcccg ccgacgggca
    11521 ctcccccgcg ttggcgctca ccggcgccgg cacgtatctg gtcgggctcg tggtgcatac
    11581 ggtcgtcggt cgccggggac cgattgcgca cgggcgttag tgttgcccgc atggccgatg
    11641 cggctcccat cgtcgacgat ctgcgcgccc agagcgacga gctggatgcg ctcgtagcgc
    11701 cgcttcccga aaaccgttgg cgtgagccaa cacccgcgca agggtggacc atcgcccacc
    11761 agatcagcca tctgctgtgg accgaccggg tggcgctgac gtccgtcacc gacgaacccg
    11821 ggttcgatga actgctctcc gcagccgccg cagacccgac gggtttcgtc gacgcagcgg
    11881 ccgaagagga gtcacgccgc tcggccgacg atctgctcgc cgactggcgg gccacccgtc
    11941 ggcgactgca cgacgcgctg ctggaggtgc cgccgacgcg aaagctggtg tggttcgggc
    12001 cgccgatgag cgcggcgtcg atggcgaccg cgcggctgat ggagacgtgg gcgcacgggc
    12061 tcgacgtcgc cgacgcgctg ggggtgcagc ggggggccgg cgagcggttg cgctcgatag
    12121 cacatctggg cgtgcgcacc cgcgactacg ccttcgccgt tcatggactg ccggttcccg
    12181 ccgagccgtt ccgcgtcgag ctgcgcgccc cggacggctc ggcctgggcg tggggtccgg
    12241 aggacgcggc gcagcggatc accggttcgg cgttggactt ctgctatctg gtcacccagc
    12301 ggcggccgct gtcagcgctt gacatcgagg ccgtgggtga cgacgcgaaa cgctggctcg
    12361 cgatcgcgca ggcgttcgcg gggccgcccg gccctgggcg ctagcagcta cagcaactgt
    12421 gcaccggtgt acgcgctcgc caggcggaca tcggtggtca acagcggaac gccaatacgc
    12481 tcggcgattt cgacgtaaag ggcgtccaca aggcgcaagt tgtcgcgccg cccccacgcg
    12541 ccgatgacaa tgtcctccaa cgtgtgccgc ttgaacggtg cactcatcag cgcagcaagg
    12601 gcgaaggcga cttcgtcgct gctcagcagc tcagcgcgcc atagccgacc cagggcggag
    12661 aggacttcgg cgtcgaagtg cgcgggcacg tgaacggtgg cctcgttcag tcgctggcga
    12721 aaactacggc ggccaaccag gagatcgacc atcgccgaag cgtcgaggac gaagccgttg
    12781 tccctcacct gccgaactcg tcgcgcgccg cgtccagcgc agcggtgatg gcgtcgcgtt
    12841 cgatcggctg gctgaccgct ggcagggtgg cgagccagcc ctgcatggag gtccgagcga
    12901 gttcggcggc gatggcggcc tgagtcagtg ccgagacgtt cagtccgtgc gtccgtgcct
    12961 gttcggccag ctcatcgggc acatagacgt tcaaccgcgc catacacaca aatatacaca
    13021 ctccgtgctg cgctacagct gtttcgaggt gtcggcggcg ccgtcgccgt tggtgtcggc
    13081 cagctcgacg tcccaccgcc cgtcgccgtc ggagtccaca tagccgagcg cgcccgacgg
    13141 tccgtcgcac aacacccggt cggccagccc gttgcggtcg gtgtcgatca cctggtcgtc
    13201 ggcctgcccg tcggcgtcga agtcgacgag cgggccgccg gtgtgctcaa taccgtcaag
    13261 cccgtaccac cgcacctggc ccgcccggtc cacagtcacc gcccacgtac ctgacccgtc
    13321 gtcggtgaag tagctttccg cggtcccgtc gtcgtccagg tccagcgcga cgtgatccgc
    13381 caggccgtcg ccgtcgagat cgccgatcgc gtcgtcaacc gagccgtcgc cgtcgaaatc
    13441 caatcgcagc gcgtccaacg acccgtctcc gtccaagtcg gtgtccggcg tgccgatcca
    13501 catcgtcgcc gaaccgtcgc cggcaccgaa gcagtactcc atgccgaggt tggacgccgg
    13561 gctactgccg ggcgttccac cacgccagca gctcggcggt agcttcctcc ttgcccatcg
    13621 ggccgcgttc catccgcagc tccttgaggt atcgccacgc ctccccgacc tgcggcccgg
    13681 ccggtatgtc aagcagctcc atgatctcgt tgccgtcgag gtcgggccgc acccgctgca
    13741 ggtcctcctt cgcggcgagc tccgcgatgc gcttctccag gtcgtcgtag ttggcctgca
    13801 gccgctcggc cctgcgcctg ttgcgggtgg tgcagtccgc ccggaccaac ttgtgcagct
    13861 tgagcagcag cggaccggcg tcggtgacgt agcgccgcac cgccgagtcg gtccacgtgc
    13921 cctccccgta gccgtggaac cgcagatgca gatagaccag ctgtgagacg tcgtcgatca
    13981 tctgcttcga gtacttcagc gtccgcatcc gcttgcgcgc catcttcgcc ccgaccacct
    14041 cgtgctggtg gaagctgacc ccgccgtcgg gttcgtgctt acgggtgtcg ggcttgccga
    14101 tgtcgtgcag cagcgcggcc aaccgcagca ccaggtcggg cccctcatcc tccagcgcaa
    14161 cagcttgccg cagcacggtc agcgaatgcc gatagacatc cttgtgctga tggtgctcgt
    14221 cgatggtcag ccgcatcgcc ccgatctccg gcagcaccac gtcgccgatg ccggtgtcga
    14281 ccatcagctc gacgccggcc accgggtcgt tacccagcat cagcttgtcc agctcggccg
    14341 ccacgcgctc gacggtgatg cggcccagct gcggtgccat cgccgcgatc gcggcccgca
    14401 cccgctgcac gacggagaag cccagttgtg agacgaaccg cgccgcccgc agcatccgca
    14461 gcgggtcgtc gccgaacgac acctcaggtg gagccggggt gtccagtacc cgctgacgga
    14521 tcgcgcccag cccgccgagg gggtcgacga attccgccgg gccgtcggcg gtaatgcgca
    14581 cggccatcgc gttggccgtg aagtcgcggc gccccagatc ctcgtcgatg ctcctgccga
    14641 accgcacctg cgggttgcgc gacacctggt cgtaggtgtc tgagcggtag gtcgtgattt
    14701 ccagccggtg cgcgtccttc gcggcgccga cggtgccgaa ctgaatcccg gtgtcccaca
    14761 acgcatccgc ccactgccgc agcatttgct ggacttgctc cggctcagcg tcggtggcga
    14821 agtccaggtc ggcgcccaac cggcccagaa tcgcgtcgcg gacgctaccg ccgaccagat
    14881 acagttcgtg gcccgcgccg gcgaacaccg caccgagggc gcgcagaatg tcggtgtgct
    14941 ggttcaacgc gatcgcggcg ccggcaagca attcggcgtc ggaagcggtt tcaggcacgt
    15001 tcgactaccc taatgcgcac tccgggtgac ccgcccaccg gcgcgtggga ccagagtgaa
    15061 atgtcatgcc aactactatc gcttgggtgt cggacggcga acgggcaagg ccgcgacggc
    15121 gccggggtcg gcgccgtggc cggcgtgccg ccgccccagc cgacaccgca cccaagcaaa
    15181 cccccgagca agctgaaacc gcgccggcca gtcccggccg ccgatcacca gaccggctgc
    15241 gcaccgtaca cgagacgtcg gccggcggat tggtggtcga cggcctggac gatccgcagg
    15301 gcagccacgt ggcggcgttg atcggccgga tcgaccggcg cggccggatg ctgtggtcgc
    15361 tgccgaaagg acacatcgaa ctcggcgaaa ccgccgagca gaccgcgatc cgcgaggtgg
    15421 ccgaggaaac cgggatccag ggcgacgtag ttgcctgcct cggcagcatc gactactggt
    15481 tcgtcaccga gggccggcgc gtgcacaaga ccgtgcacca ctatctcttg cgtttcctcg
    15541 gcggggagct gtccgaccac gacctcgagg tcagtgaggt ggcctgggtg ccgctggacc
    15601 agctgccgtc gcggctggca tacgccgacg aacgccgact cgctgaggtc gccgacgaac
    15661 tgattcagac gttgcacacg cacgggccgg ccgccctgcc gccgctgccg tacagcgcgc
    15721 cgtggcgccg gcgtcagacc cattcacgca cccgccggcg tcgttccgat gagaccgagt
    15781 cgggccggac gaacggtcac aggcaccagc agtgacacac cggcggcgcc gcgcatcact
    15841 gcggttcacc gcagcaacga cactgcgggt catcgccgcg gcggtgctcg tcgcgttccc
    15901 gctgctggcg accgtcatcc cccgcgcggt ggcgggcgaa cctggcgacg tgcgcttcct
    15961 ggaggtgcgc atcgaccgcg tcaccccgga gatcgtcacc accaccgggg acgccaccgt
    16021 gaccgtgacc ggcaccgtga ccaacgtcgg cgaccggtcg gtgcgtgaca tcgttgtccg
    16081 gctcgagcac gccggggagg tcacgtcgcc gcaggttctg cggaccagtc tcgacggtgc
    16141 caccgaccag taccagccgg tcagcgactt cgtcaccgtg gcgcccgagc tgcgacgtgg
    16201 gcagtccggc gctttcacgc tgtcgtaccc cgtgcgctcc acgtcggcgc cgtcgctcgg
    16261 catcaacgaa cccggggtgt acccgctgct cgtcaacgtc aacggcacac ccgactacgg
    16321 cgaacccgcg cgcctcgacg acgcccgttt cctgctaccg gtgctgggcg tgccggctga
    16381 cccctcggtc gagtcgaccg acacgctgac cagcgtcgtg gcgcccgaca ccgccaaacc
    16441 cgtgctgatc acgatgctgt ggccgttggc ggaccggccg cggttggcgc cgggcgcgcc
    16501 gggcggtgtc acaccggtgc gactggtcga cgacgagctg gcagtctcgc tggctcccgg
    16561 cggccggctc gacacactgt tgtcggcggc ggacttcgcg accgggccat cgatcgaccc
    16621 ggatggtcgc gtcaacggcg cgctatgtct ggccgtcgac ccggacctgc tggtgaccgt
    16681 caacgcgatg accgccggct acgtcgtcgc cgacaacccc gacgaccgaa actcccccac
    16741 ccaccccggg gctggacagg cagcggcggc ggcgtggctc gaccggctgc gcacgcttgc
    16801 ccgccggatg tgtgtggccc cgacgccgta cgcgcaggcc gatctcgacg ccttgcaccg
    16861 ggtcggcgac ccggggctgg cgacgatcgc gaccaacggc gccggcgaca tcgtcgacca
    16921 gatcctgggc gtcacgtcca cccgcggcgc cacactgctc ggcgacgcgt cgctgaccga
    16981 gggggccgca gacctgctga acgcccaagg cccgacggtc gcgatcgccg ccgcgaactg
    17041 caccgcacaa gacgaggccg acggcaaact gcagccggcg aacgtcaacc cgcaccgact
    17101 gtcagaccag ctggtgaccg ctccgttcga tcccacggtc ggcgccgcgc tcgcgggcgc
    17161 ggggcgcaca ccagcgtcgc cgtcctatct ggactactcg ctcgtcgtgc cgtacaagca
    17221 cgactccgcg acggcgcggc gccaggacgc gatcagcgcg atgttgtggc tcggcctgaa
    17281 ccccgacgcc gcgccgcgca gtcagattct gatgccgccg ctgttctggg atctgcaggg
    17341 cgcagatgcc caggcgctcc tgaccgcgct ggccaccaca attcgcgccg gcctagcggt
    17401 tccgcggccg ttgcccgcgg tgatcggcga cgccgaagcc gccgctgtcc ccagttcgca
    17461 agcgccgggc aaggcagcgc agcgcgggcg cgtcgacgac gcggtggcct ccgacatgcg
    17521 cgagcaggtt ggcaggctgt ggggactgac cgcagcgttg acgacggatc cgcggaccgg
    17581 tttgacgggc acgcagtaca ccgcgccgct gcgggaggac atactgcgcg cgttgagcca
    17641 agcgtttccg cccgacggcc gcaacgagca ggcgcagcag cgcatggccg cggtcaacgt
    17701 caccgtcgac gacatgttcg gcgcggtcac gatcgtcaac cccggcggct cgtacacgct
    17761 ggccaccgaa cacagcccgc tgccgctggc gctgcgcaac gaccttccgg tgccgatcaa
    17821 ggtgcggctt caggtcgacg cgccgcccgg aatgacggtg accgacatcg gtgtgcagga
    17881 gctgccgccg ggatacctgc cgctgcgcgt gccgatcgag gtgcacttca cgcaacgcgt
    17941 cgcggtggac gtcgcactgc gcactcccga cggcctgttg ctgggtgagc cggtgcggtt
    18001 gtcggtgcac tccaacgcat acggcaaggt gctgttcgcg atcacgatga ccgcggcggc
    18061 ggttctggtg gtcctggcag gccggcggct gtggcaccgc ttccgcggcc agcccgaccc
    18121 ggctgacctc gaccgcccgg aaccagagtg accgccgccg gccggcgacc cgagctcacc
    18181 gacgcggcgg tggtgtcgcg gtcgtgggga atggcgttcg cgacgttggt cagccgcatc
    18241 accggcttca ccaggatcgt gctgctcgcc gcgattctcg gtgcggcgct gtccagtgcg
    18301 ttctcggtgg ccaaccagct gcccaacctc gtcgccgcgc tcgtgctgga ggccaccttc
    18361 accgcgatct tcgtgcccgt gctggcgcgc gcggaacgcg acgacgccga cggcggcgcc
    18421 accttcgtcc gtcggctcgt cacgctcgcg acgacgctgc tgctgatcac caccgcgctg
    18481 tccgtcgtgg ccgccccgct actggtccgg ttgatgctgg gcagtgaccc gcaggtggac
    18541 aaacagctca ccgtcgcgtt cgcgtatcta cttcttcctc aagtactttt ctacggactg
    18601 tcatcggtgt tcatggcaat actgaacacc cgcaatgtgt tcggcccgcc agcgtgggcg
    18661 ccggtggtca acaacatcgt cgcgatcgtc actctcggtg tgtatgtcct tgtcccaggc
    18721 gaactttcgg tggagcccgg caacaccaag cttcttgtgc tgggcatcgg caccacgctc
    18781 ggggtggtcg cgcagagcgt cgtcctgctg atcgcgatca agcgcgaacg catcagcctc
    18841 cgcccgctgt ggggcgtcga cgaccggctc aagcagttcg ggaccatggc cggcgcgatg
    18901 gtgctctacg tgttgatcag ccagctcggt ttggtcgtcg gcaaccaaat cgcaagctgg
    18961 gcagcggctt ccgggcctgc gatctacaac tacacgtggc tggtcttgat gttgccgttc
    19021 gggatgatcg gtgtgacggt gctgacggtc gtcatgccgc ggctgtcccg caacgccgcc
    19081 gctgacgaca tcccggctgt gctggacgat ttggcgcttg ccactcgcct gaccatggtg
    19141 acgctgatcc ccaccgtcgc gttcatgacc gtgggcggcg cggccatcgg ctccgcgttg
    19201 tttgcatacg gcaagttcgg ctccgtcgac gccagctatc tcggaagcgc cattgcgctg
    19261 tccgctttcg cgctgattcc gtacgcgatg gtgctgctgc agttgcgggt gttctacgcg
    19321 cggcagcagc catggacgcc gatcgcgatc atcgtcgtga tcaccacggt caaggtcgcg
    19381 gcctcgctgg gggtacaaca gctcaccgag caccacgtga tcagcaaccc cgaaatggtc
    19441 gccggctacc tcgggctggc caatgggctc ggctttctgg ccggtgcgat agccggctac
    19501 ctgctgctgc gcccggcgct caacccgccg agtgggtaca tgttcagtgc ggacgtggtg
    19561 cgcaccgtgc tcgtcaccat cgccgcgtcg ttgctggcgg cgatggtcgc gtatatcgcg
    19621 gaccagctgc tgcacttcca gaacttgacc gaaagcggcg ggggcgccgg ttcgctgctg
    19681 cggctgttgc tgcttggctc gatcatgctt ccgatcgtgg ccgcggtgat gctcgccgcc
    19741 ggtgtcgcag aagcgcatgc ggccctcgcg gcggtccggc ggcggctcgg cagagccgcc
    19801 gtgaaatcat cgcctgcgtc aagtccggtt tcggaccggc cccgctcgga tacctcggtc
    19861 acgtaccctg aacagaggct ttcgttcgcg cagaggccgc gggccgacgt gcaggaaggg
    19921 atggcggtga ccaacgagac cgcgggcagt cccgatgcga ctgcccggct gacgcggcca
    19981 tccgccgacg atttccagcc cgatgtggcc gaatctcccg ccgacaccag ggcgacatct
    20041 cccgccgaca ccagggcgac caccgaggtg cccatgcgcg gccgctcgcc gggcgacttc
    20101 gccggcgacc ccacccgcga atcgatgggg atcgccgcac cccgcgacac ggcggactcg
    20161 tcgcgctctg accaagaggg ccatctgata ccgggtgcca gcatcgccga cggccgctac
    20221 cggttgttgg tgttccacgg cgggcccgag cacctgcagt tctggcaggc gttggacacc
    20281 gcgctcaacc ggcaggtggc gttgaccttc gtcgaccccg aaggacagct gcccgtcgac
    20341 agggtgcagg acatcctcgc gcgcacgctg cggctgagcc ggatcaccat gcccggcatc
    20401 gcgcgcgttc tcgatgtcgc gcgcgcacgc accggcggcc tagtggtgtc ggagtggatc
    20461 cgcggcggct cgctgcagga ggtcgccgac accttgccgt caccggtggg cggtgcccgg
    20521 gccatgcagt cgctggcgac cgccgccgac gccgcgcacc gggcgggcgt cgcactgtcg
    20581 atcgatcacc ccagccgggt gcgggtcagc atcgagggcg acgtggccct ggccttccct
    20641 gcgaccctgc cgggcgcgaa tcccgacgcc gacctgcgcg gtatcggcgc ggcgttgtat
    20701 gcgctgctgg tcaatcggtg gccactgccc gagagcggcg tgcccagcgg atttgcgccg
    20761 gcggactccg acccggccgg aaatccggtc gagcccaagg ccgtcaaccg cgacatcccg
    20821 ttccagatct ccgcagccgc cgtgcggtcg attcaggtca acggcggaat caccagcgcg
    20881 gctacacttt cgcatcttct gcagcaggcc accgcggagg ccgaccgcac cgacctgatc
    20941 actccggtcg aagaaccgct gcctgcgtct ccgtcgcgat tccggggcgc aggtttccgc
    21001 ggctgggggg acttggacga agaggagcgc acccggcgcc gcaagggcct tctcatcggt
    21061 ttaggtgtcg taggcgcggt cgtcgtcgtg gcgacattgc ttgccgcagc gctgttcaac
    21121 ggcgtcggcg acgactattc gagcaccgag aaccagctgg gcctgaaccc gacgagcggc
    21181 ctccctgcgc cagcgggcaa cggcggggac gtgaaacccg ttggcgccgc ggtgttttcc
    21241 cccgatggcg gcgcagacga tcccgataac gcgggccggg cggtcgacgg caacacgtcg
    21301 accgcatggg ccaccgacgt ctacaccaac agcgtgccgt tcccgaagtt caagaacggc
    21361 gtcggcctga tgctggagct gccgaagtcg acgacgctca gcgcagtgga cgtcaatgtg
    21421 tcgagcaccg gcacgaagat tcagatccgg tcggcgacca ctgcctcgcc cagcagcctg
    21481 gaggacacga ccgcgctcac ggagcccacg tcggtgcagc ccggcccgaa ccggataccc
    21541 gtcacgatgt cctcccccac gtcgtatgtg ctggtgtgga tttcgacgtt gggcaccact
    21601 gacggcgaga gccaagcgga tatctacgag atcactctcg aaggcacctc ctgacattca
    21661 tcccgagtaa tctcattgat gatgttttcg ggctgcctgc accgcaccag cgaacggttt
    21721 tcgggagctt gacttcacgt atatccctac cgagctaacc gccgccccgc ttccgatagt
    21781 tgcagtcgac aagactttcc cagttggcgt ccggggccag gtctgcaccc gcttagggtt
    21841 cggctgtggg gcgtttcggg ggggcacagc agccgcaacg gtccgacgcg gagttgctcg
    21901 ccgcacacgt cgggggcgat cgctacgcct tcgaggagct gttcctgcga caccaccgtc
    21961 agctgtaccg gctagcccgg ctgaccagcc gcaccagcga agacgccgcc gacgcgctcc
    22021 aggacgcgat gctttccgcg caccgcagtg ccgcatcctt ccgcaacgac gccgcggtaa
    22081 gcagctggct gtaccgcatc gtcgtcaacg cgtgcctgga caggttgcgg cgcaacaaga
    22141 ttcacctcac tgtctgcatt gaggacgacg cctacccggt cagcgactcg acgtcgcgtt
    22201 cggacaccgc agtcgtcgtg cagcgggcgt tgatgcggct gcccgtggac cagcgggccg
    22261 ccgtcgtcgc ggtcgacatg cagggctact ccgtcgccga cgccgcccgg ctgctcggcg
    22321 tcccgcaggg aaccgtgaag agccgatgcg cccgggcgcg agcgcgactc gcgctgatgc
    22381 tgcgctactt cgaccaggac gcagaaagcg gggccgcagg gtaaaacggg ctggtggaca
    22441 cgaagcgagg ctcgtccgag cgacaatcaa gctccgacgg ccccgcgatc gagcgtgtcc
    22501 gcgccgagct ggccgggctg agcgacgacg cggcatcggc tcccgacgtg ccgcggccgg
    22561 tcgccgagcg cgtggtgtcc gcccttcgga ccgcgcctcc ggttcgacgc acgcccgcgg
    22621 tccggatcag tgccgcggtg ggcgtcgcgg cggcgctggt ggcggccgga atcgggacgg
    22681 tcatgctgca gcgctcgggc tcgtctgtgc cgccgaccga atcgtcggcc aagcatctgc
    22741 ccgagccggc cgcggcgacg gtgccgcttt ccggcgacga gatcgtcgcg ctgctgagcc
    22801 gtcctcccga cctcggggca ctcggcgatg cgcggcggcg gtcctcgtgt ctgagcgggc
    22861 tgggctatcc ggggccggac gccgtgctcg gcggccagca agtcgatatc aacggtgcgc
    22921 cggcggtttt gctggtgctg ccgggcgaca ccccggatac cgtcgccgcg ctggcggtac
    22981 gaccgaactg cagctcgatc gacacggggc tgctcgccga cacccaaatt cggcgtccgt
    23041 agcgccggcc gggaacacct ggacctaccc tggtgttgca tcgatcgctg tccgttggca
    23101 gaaaggctgg catgacgtca tcccccaccg tccacgatgt gatcatcatc ggctcgggac
    23161 cagccggcta caccgcagcg atctacgccg cacgggcgca gttggccccg ttggtcttcg
    23221 aaggcagcca gttcggcggc gcattgatga ccaccaccga cgtcgagaat tatccgggtt
    23281 ttcgtgccgg aatcaccggc cctgaactga tggacgagat gcgggagcag gctctgcgat
    23341 tcggtgccga cctgcggatg gaggacgtcg acgcggtgtc cctgcagggt ccggtcaaga
    23401 ccgtcgctgt cggcgacgaa actcaccggg cccgcgcggt catcctcgcg atgggtgccg
    23461 ccccgcgcta cctcggggtg cccggggagc agaaccttct tggccgcgga gtcagctcgt
    23521 gtgcgacctg cgacggcttc tttttccgcg accaggacat cgcggtgatc ggcggaggcg
    23581 actcggcgat ggaggaggcg acgttcctca cccggttcgc gcgaagtgtc acgatcgtgc
    23641 accgccgtga ggagttccgc gcgtcgaaga tcatgctcaa gcgggcagag gacaacgaca
    23701 agatccgttt cctcaccaac accgtggtca ccgaagtcct gggcaccgac agcgtcaccg
    23761 ggttgcggtt gcacaacact ctcaccggcg aggacagcac cctcggcgtc accggcgtgt
    23821 tcgtcgccgt cggccacgac ccgcgctctg aactggtccg cgaccagctc gacgtcgatc
    23881 gcgacggtta cgttctggtc cggggtcgca gcacctatac ctcgatcgac ggagtgttcg
    23941 cggccggcga cctcgtcgac cgcacgtatc ggcaggcggt gaccgccgcc ggtaccggct
    24001 gctcggcttc catcgacgcc gagcgctggt tagccgaaac cgcccatgtc gacaccgacg
    24061 acaacctgat aggagcgccg ctgtgagcga caccaccgcc accatcgccg tcaccgacga
    24121 ctcgttcgcg caggacgtgc tcgccaacga gcgaccggta ctcgtcgact tctgggcgac
    24181 ctggtgctca ccgtgccgga tggtcgcgcc ggtgctggag gagattgccg ccgaaaaggc
    24241 cggcgcgctc accgtcgcca agatcgacat cgacgccaat cccggcacgg cacgcgactt
    24301 tcaggtcatc tcgattccga cgctgatctt gttcaagggc ggcgagcc
//
LOCUS       DASWBF010000338        14102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_37444, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000338 DASWBF010000000
VERSION     DASWBF010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37444"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VF874_12430"
     CDS             <1..404
                     /locus_tag="VF874_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9176836.1"
                     /translation="AEDNDKIRFLTNTVVTEVLGTDSVTRLRLHTTLTGEDSTLGVTG
                     VFVAVGHDPRSELVRDQLDVDRDGYVLVRGRSTYTSVDGVFAAGDLVDRTYRQAVTAA
                     GTGCSASIDAERWLAETTHVDTDDNLIGAPL"
     gene            401..733
                     /gene="trxA"
                     /locus_tag="VF874_12435"
     CDS             401..733
                     /gene="trxA"
                     /locus_tag="VF874_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9176837.1"
                     /translation="MSDTTATIAVTDDSFAQDVLANERPVLVDFWATWCSPCRMVAPV
                     LEEIAAEKADALTVAKIDIDANPGTARDFQVISIPTLILFKGGEPVKRIVGAKSKAAL
                     LRELADDI"
     gene            900..2093
                     /locus_tag="VF874_12440"
     CDS             900..2093
                     /locus_tag="VF874_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9176838.1"
                     /translation="MSTLRRGDRGSAVTEIRSALASLGWLEDPDADLTTGKHAVPEMF
                     DAELDQAVRAFQQHRGLLVDGVVGEATYRALKEASYRLGARILHHQFGAPMYGDDVAS
                     LQARLQDLGFYTGLVDGHFGLHTHNALMSYQREYGLTSDGICGPETLRSLYFLGSRVT
                     GSSPHAIREEELVRRSGPRLSGKRIIIDPGRGGDDHGAIMQGPAGPISESDILWDLAS
                     RLEGRMTAIGMETFLSRPANRSPSDAERAATANTVGADLMIGLRCATQPSPLPNGVAS
                     FHFGNSHGSVSTIGRNLADFIQREVVARTGLQDCRTHGRTWDLLRLTRMPTVQVDIGY
                     ITNPRDRAMLVTTQTRDSIAEGILAAVKRLYLLGKNDRPTGTFTFAELLAHELSVEQA
                     GRLSR"
     gene            complement(2083..2829)
                     /locus_tag="VF874_12445"
     CDS             complement(2083..2829)
                     /locus_tag="VF874_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX9176839.1"
                     /translation="MSARIMPLRLECFEQLPKHARRCVFWEVDPTALGRDDRFADPEF
                     EKEAWLSMVMLEWGACGQIAVPIREHGDDQDPEHSEAPCVGYALYAPPGAVPRAGSFP
                     TSPVSPDAVLLTSMGVEPGQPDTLARSLVAQAVADLIRRGVRALEAFGRTAEAEELVD
                     HRTAPPDVRSVVQPFGDCSFDQCMTGADFLLDVGFTVVAPHRYFPRLRLELDQGLGWK
                     AEVEAALDRLLQSEQLEPVGAGAGRVDATG"
     gene            complement(3057..4028)
                     /locus_tag="VF874_12450"
     CDS             complement(3057..4028)
                     /locus_tag="VF874_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9176840.1"
                     /translation="MTQASRRKGGLGRGLASLIPTGPSETGGAGLGPRLGDAAADVVL
                     GGTPAGDRASVGAVYREIDPALIVPNPRQPRQVFDEEALAELVHSIREFGLMQPIVVR
                     SPDPAGRYQLVLGERRWRAAQQAGLATIPAIVRETSDDNMLRDALLENIHRVQLNPLE
                     EAAAYQQLLEEFGVTHDELAARIGRSRPLVTNMIRLLRLPIAVQRRVAAGVLSAGHAR
                     ALLTLEGGPDAQEELAARIVAEGLSVRATEEAVTLANRGDSTPAPGARRKPIQMPGLQ
                     DIAERLSSNFDTRVTVSLGKRKGKIVVEFGSVDDLQRIVSLMNAADG"
     gene            complement(4054..4947)
                     /locus_tag="VF874_12455"
     CDS             complement(4054..4947)
                     /locus_tag="VF874_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9176841.1"
                     /translation="MANVSRETDTPIGAAAERAMHVLHTIHPSLPRPAHRRLFTVANQ
                     KGGVGKTTTAVNLAAALALQGLKTLVIDLDPQGNASTALGIDHREAGTPSSYEVLLGE
                     IKLADALRRSPHSDRLFCVPATIDLAGAEIELVSMVARENRLRTALAELDRTDFEYVF
                     IDCPPSLGLLTINALVAAPEVLIPIQCEYYALEGVSQLMNNIEMVKAHLNPALTVSTV
                     VLTMYDGRTKLADQVAGEVRRYFGDKVLRTVIPRSVKVSEAPGYSMTIIDYDPGSRGA
                     MSYLDASRELAERDGPPQTGA"
     gene            complement(4980..5684)
                     /gene="rsmG"
                     /locus_tag="VF874_12460"
     CDS             complement(4980..5684)
                     /gene="rsmG"
                     /locus_tag="VF874_12460"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX9176842.1"
                     /translation="MFHVKHGEPPGAPPAAQKVFGDRLDLAVRYAGMLATDGVKRGLI
                     GPREAERVWERHLLNSAAVESLIADGARVADVGSGAGLPGIPLALARPDLRMTLIEPM
                     LRRTTFLREVVDELRLDVEIVRGRAEDPVVRQRVGEVDVVVCRAVAAQDKLTEWCLPL
                     LRDGGLLLAVKGDRADEEVVQHRRVMESLGAEAVRVVRCGGDYLEPPTTVVVAERTAA
                     SRAQASQARARSGRRS"
     gene            complement(5751..6293)
                     /locus_tag="VF874_12465"
     CDS             complement(5751..6293)
                     /locus_tag="VF874_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEX9176843.1"
                     /translation="MTDADTAERDDELNDATAETPKPEDLEERLVTEGEIAGDYLEEL
                     LDVLDFDGDIDLDVEGNRAVVSIDGGDDLVKLVGRRGEVLDALQELTRLAVHQKTGER
                     SRLMLDIASWRRKRRDELAALGHKVAQRVLETGTREQLAPMTPFERKIVHDAVAAVSG
                     VRSESEGQEPSRRVVVLADQ"
     gene            complement(6345..7400)
                     /gene="yidC"
                     /locus_tag="VF874_12470"
     CDS             complement(6345..7400)
                     /gene="yidC"
                     /locus_tag="VF874_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9176844.1"
                     /translation="MWVWYKAFAFLLGPTNFFTWALTVMFLVFTLRAILYKPFVRQIR
                     TTRQMQEMQPQIKALQKKYGKDRQRLALEMQKLQREHGVNPILGCLPMLAQIPVFLGL
                     YHVLMSFNRTQHGIGRLGLDPETNRSLGNYVFSPTDVGHFLDANLFGAPLGASMTQTS
                     GLEAFKNFDFSRPALIAIGVPMMLLAGFATHLNSRASIARQSPEAAANPQTAMMNRLA
                     LYIFPLGVVVGGPFLPLAIIIYWFSNNIWTFGQQHYVFGKIAHEEEEKKQEAIARRAA
                     NAPAPGAKPKRQPKKSAAASSDEAEITDSSGVELSDVDGGGPTDEEPKPNAARANGAG
                     SVGRTPRPGVRPKKRKR"
     gene            complement(7444..7815)
                     /gene="yidD"
                     /locus_tag="VF874_12475"
     CDS             complement(7444..7815)
                     /gene="yidD"
                     /locus_tag="VF874_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9176845.1"
                     /translation="MTSAVTRMRRLPARAVILVIELYRRYVSPMRLPTCRFMPTCSQY
                     AVEALSECGLVRGGWLAAVRLAKCGPWHRGGWDPIPDRHNELHRRGDERLARRAGRAD
                     GNAVDNVWDTPAQRGETTSCV"
     gene            complement(7812..8171)
                     /gene="rnpA"
                     /locus_tag="VF874_12480"
     CDS             complement(7812..8171)
                     /gene="rnpA"
                     /locus_tag="VF874_12480"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX9176846.1"
                     /translation="MLPARYRMTRSAEFGSTVNNGVRAVERDLVVHAQRCNRQGEPGP
                     RIGLVVGKSVGSAVQRHRVARRLRHVALSLVTELGPADRIVIRALPSSRHAISARLAQ
                     ELRSGLTRAHVLPKGQR"
     gene            complement(8194..8337)
                     /gene="rpmH"
                     /locus_tag="VF874_12485"
     CDS             complement(8194..8337)
                     /gene="rpmH"
                     /locus_tag="VF874_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX9176847.1"
                     /translation="MAKGKRTFQPNNRRRARVHGFRLRMRTRAGRAIVSNRRRKGRRT
                     LTA"
     gene            8908..10407
                     /gene="dnaA"
                     /locus_tag="VF874_12490"
     CDS             8908..10407
                     /gene="dnaA"
                     /locus_tag="VF874_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX9176848.1"
                     /translation="MTADPDAPFAAVWNTVVSELNSDQPDGPSTTNDDRTTPPLTPQQ
                     RAWLTLVKPLTIAEGFALLSVPSSFVQNEIERHLRTQIVDALSRRLGHRVELGVRIAP
                     PEGGDADDLLAGLSPTYDTDLDEVDDDTDALASAHESWPSYFLNKPPQDAGQPQGVSL
                     NKRYTFDTFVIGASNRFAHAAALAIAEAPARAYNPLFIWGESGLGKTHLLHAAGNYAQ
                     RLFPGMRLKYVSTEEFTNDFINSLRDDRKVAFKRSYRDIDVLLVDDIQFIEGKEGIQE
                     EFFHTFNTLHNANKQIVISSDRPPKQLATLEDRLRTRFEWGLITDVQPPELETRIAIL
                     RKKAQMDRLDAPDDVLELIASSIERNIRELEGALIRVTAFASLNQTPIDRALAEIVLR
                     DLISDAESMQISTAMIMAATAEYFETNVEELRGPSKTRPLAQARQIAMYLCRELTDLS
                     LPKIGQAFGRDHTTVIYAERKIRGEMAQRRDVFDQVKELTTRIRQRSKR"
     gene            10907..12100
                     /gene="dnaN"
                     /locus_tag="VF874_12495"
     CDS             10907..12100
                     /gene="dnaN"
                     /locus_tag="VF874_12495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX9176849.1"
                     /translation="MATTTVGLTDLKFRLVREDFADAVAWVARNLPTRPAVPVLAGVL
                     LAGTDAGLTISGFDYEVSGEVQVPAEIATPGKVLVSGRLLSDITRALPAKPVDVSVDG
                     TRVSLTCGSARFSLPTMAVEDYPSLPALPEETGVISADLFTEAIGQVAVAAGRDDTLP
                     MLTGIRVEISGESVVLAATDRFRLAVRELTWSTTSADIEAAVLVPARTLAEVAKAGIT
                     GADVALALGAGQTVGKEGLLGIRSEGKRSTTRLLDAEFPKFRQLLPTEHTAVATVEVA
                     ELSEAIKRVALVADRGAQVRMEFSEGTLRLAAGADDVGRAEEDLPVEFSGEPLTIAFN
                     PTYLTDGLSSLNSARVSFGFTTPSRPAVLRPATDEDGSNGGYGPFPAVQSDYVYLLMP
                     VRLPG"
     gene            12129..13022
                     /gene="gnd"
                     /locus_tag="VF874_12500"
     CDS             12129..13022
                     /gene="gnd"
                     /locus_tag="VF874_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005087665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX9176850.1"
                     /translation="MQLGLVGLGKMGFNMRERLRDGGHEVVGYDPRPEVTDVPTLAAL
                     AGALATPRVVWVMVPSGPVTNETIVALAAELGEGDLVIDGGNSRYTEDAPHAKLLDEK
                     GIAFIDAGVSGGVWGLKEGYGLMVGGSEANVARAMPIFDTLRPPGPREDGFVHAGPVG
                     AGHFAKMVHNGVEYALMTAYAEGYEILTAEDLIQDPQAVYQAWTNGTVVRSWLQQLLA
                     KALKEDPNLAEIAGYTEDSGEGRWTVEEAIRLRVPVPGISASLFARFLSRQDDSPTMK
                     AVAALRNQFGGHAVHRVSQSG"
     gene            13034..>14102
                     /gene="recF"
                     /locus_tag="VF874_12505"
     CDS             13034..>14102
                     /gene="recF"
                     /locus_tag="VF874_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX9176851.1"
                     /translation="MYVRHLGLRDFRSWPQADIELEPGRTVFVGPNGYGKTNLIEALW
                     YSATLGSHRVATDAPLVRAGTSRAVVSTIVVNDGRELAVDLEITPGKANKARLNRSPV
                     RSPREVLGVLRAVLFAPEDLALVRGDPGERRRYLDELATVRRPALAAVRADYDRVVRQ
                     RTALLKTAAGQRPRDAGLLQTLEVWDGHLAEHGARLLAARVALVNELRPEVEKAYQLL
                     APGSRAADIRYRSSVEFDAGGDGDSFETALLAALAARRDAELDRGVCLVGPHRDDLEL
                     VLGDQIAKGFASHGESWSMALSLRLAAYELLRSEGTDPVLLLDDVFAELDTARRRSLA
                     VVAGEAEQVLLTAAVPDDIPLD"
BASE COUNT         2420 a         4820 c         4413 g         2449 t
ORIGIN      
        1 gggcagagga caacgacaag atccgcttcc tcaccaacac cgtggtcacc gaagtcctgg
       61 gcaccgacag cgtcaccagg ttgcggttac acaccacgct caccggcgag gacagcaccc
      121 tcggcgtcac cggcgtgttc gtcgccgtcg gccacgaccc gcgctctgaa ctggtccgcg
      181 accagctcga cgtcgatcgc gacggttacg ttctggtgcg gggtcgcagc acctatacct
      241 cggtcgacgg agtgttcgcg gccggcgacc tcgtcgaccg cacgtatcgg caggcggtga
      301 ccgccgccgg taccggctgc tcggcttcca tcgacgccga gcgctggttg gccgaaacca
      361 cccatgtcga caccgacgac aacctgatag gagccccgct gtgagcgaca ccaccgccac
      421 catcgccgtc accgacgact cgttcgcgca agacgtgctc gccaacgagc ggccggtact
      481 cgtcgacttc tgggcgacgt ggtgctcacc atgtcggatg gtcgcgccgg tgctggagga
      541 gatcgccgcc gagaaggccg acgcgctcac cgtcgccaag atcgacatcg acgccaatcc
      601 cggcacggca cgcgactttc aggtcatctc gattccgacg ctgatcttgt tcaagggcgg
      661 cgagccggtg aagcggatcg tcggcgccaa gagcaaggcc gcgctgctac gcgaactcgc
      721 tgacgacatc tagccgacag gccgtcgaca ggtcgcctgg cgttttctcg attcggggaa
      781 tcgtctgcga caataatgct tagcttgtcg tgcgatcgcc cgattccgac gctgccagcg
      841 caaacggctc gtcgccgcgt cggtgtccgg cacggcgacc gtgaccgaaa ggcccctcta
      901 tgtcgactct gcgccgcggt gaccgcggca gcgcagtcac cgagattcgc agcgccttgg
      961 cgtcactggg atggttggag gacccggacg cggacctgac gaccggcaag cacgcggtcc
     1021 ccgagatgtt cgacgcagaa ctcgaccagg cggtgcgtgc ctttcagcag catcgcggcc
     1081 tgctcgtgga cggtgtggtg ggcgaagcca cctatcgcgc gctgaaggaa gcgtcgtacc
     1141 gcctcggtgc gcgcattctg catcatcagt tcggcgcgcc gatgtacggg gacgacgtcg
     1201 cgtcacttca ggctcggctg caggatctgg gtttctacac gggtttggtc gacggtcatt
     1261 ttggcctgca cacccacaac gcgctgatgt cctaccaacg cgagtacgga ctcacatccg
     1321 acggcatctg cggcccagaa acgttgcgct cgttgtattt tctcggttcc cgcgtgacgg
     1381 gtagctcacc gcacgcgatt cgcgaggagg agctggttcg ccgatccggg ccacggcttt
     1441 cgggcaagcg catcatcatc gacccgggcc gcggcggcga tgaccacggt gcgatcatgc
     1501 aaggtccggc cggtccgatc agcgagtcag acatcttgtg ggacttagct tctcggttgg
     1561 aaggccgaat gacggccatc ggcatggaga cgttcttgtc ccggccggcc aaccgcagcc
     1621 cgtcggatgc tgaacgtgcc gcgaccgcga acacggtagg cgccgacttg atgatcggtt
     1681 tgcgttgcgc tactcagccg agcccgctgc ccaacggcgt cgcgtcattt cacttcggca
     1741 actcacacgg ctcggtgtcc acgatcggtc gcaacctcgc cgacttcatt caacgagaag
     1801 tggtggcccg caccggatta caggactgcc ggacacacgg acgcacgtgg gacctgctcc
     1861 ggctcacccg catgccgacg gtgcaggtcg acatcggcta catcaccaac ccccgcgatc
     1921 gcgcgatgct ggtgaccacc cagacccgcg actcgatcgc cgaggggatc ctggcggccg
     1981 tcaagaggct gtatctcctc ggcaagaacg accgtcccac aggcactttc acgttcgccg
     2041 aattgctggc gcatgagttg tcggtggagc aagccggccg gctcagccgg tagcgtccac
     2101 ccggccggcc cccgctccaa ccggctccag ctgctcgctc tgcaacagcc gatccagcgc
     2161 agcctccact tcggccttcc aacccaggcc ctggtccagc tcgaggcgca gccgcgggaa
     2221 gtaccggtgc ggtgcgacaa cggtgaagcc gacgtccaac aggaaatccg ctccggtcat
     2281 gcactggtcg aacgagcagt cgccgaacgg ctgtaccacc gagcggacgt ccggcggagc
     2341 ggtgcgatgg tctaccaact cctcggcctc tgcggtccgc ccgaacgcct ccaacgcgcg
     2401 gactccgcgt cggatcagat ccgcgaccgc ttgcgcgacg aggctgcggg ccagggtgtc
     2461 gggctgcccg ggctcgacgc ccatcgacgt caagagcacc gcgtcggggc tgacgggcga
     2521 ggtgggaaac gagccggcgc gcggaactgc gcccggcggc gcgtatagcg catagccgac
     2581 gcacggggct tccgagtgct ccgggtcttg gtcgtcgccg tgttcgcgaa tcggtaccgc
     2641 gatttgtccg catgcacccc actcgagcat gaccatcgac agccacgctt ccttctcgaa
     2701 ttccgggtcg gcgaagcgat cgtcgcgacc gagagctgtt gggtcgacct cccagaacac
     2761 gcagcggcgg gcgtgcttgg gcagctgctc gaaacattcg agccgcagcg gcatgattcg
     2821 ggcggacact acgctctcgg acctccaaag ggtgctgctg cccaaggcag ccctaccaga
     2881 ataagagagg gtgggcgttg taggccagtt tcggccgtct tgcatgttcg atctcctgca
     2941 cagcgcgaaa atccttgaga cacgcaatct ttcgtctcaa ggtcacttgc gggtcgcgaa
     3001 ccggtcagga tgccgtcaca gtgacgtatc gggtcggtgt cactgggagg cgaaaatcag
     3061 ccatcagcgg cgttcatcag gctcacgatc cgctgcaagt cgtcgaccga accgaactcg
     3121 accacgatct tgcccttgcg cttgcccaga ctcacggtga cccgggtgtc gaagttgctc
     3181 gacagccgct cggcgatgtc ttgcaggcct ggcatctgaa tcggtttgcg acgggcaccc
     3241 ggcgcgggtg tgctgtcgcc gcggttggcc aacgtgacgg cctcctcggt ggcgcgcacc
     3301 gacagcccct cggcgacgat tcgcgcggcc agttcttcct gcgcatcggg gccaccctcc
     3361 aaggtcagga gcgcgcgggc atggcccgcc gacagcaccc ccgcggcgac tcgacgctgc
     3421 acggcaatcg gcaatcgcag cagccggatc atgttcgtga ccagcggacg cgaccggccg
     3481 atccgcgcgg cgagctcgtc gtgggtgacg ccgaactcct ctagcagctg ctggtaggcg
     3541 gcggcctctt caagaggatt caactgcacg cggtggatgt tctccagcag ggcatcgcgc
     3601 aacatgttgt cgtcgctcgt ctcgcggacg atggctggaa tcgtcgccag tcccgcctgc
     3661 tgcgcggcgc gccagcgccg ctcccccaac acgagctggt accggccggc cgggtcgggt
     3721 gagcgcacga cgatcggttg catcaacccg aactcgcgga tcgagtgcac cagctccgcg
     3781 agcgcctctt cgtcgaacac ctgccgtggt tgccggggat tcgggacgat cagcgccggg
     3841 tcgatctcac ggtaaacggc gccgacggac gcgcggtcac cggcgggggt accgccaagc
     3901 acgacgtccg cggcggcatc gcccagcctc gggcccaacc ctgcgccacc cgtctccgac
     3961 ggccccgtcg ggatcagcga tgccagcccg cgaccgagtc cgcccttccg gcgcgatgct
     4021 tgggtcatct atgcctcctt ttcacaccag cggtcatgcg cccgtttgcg gcggcccgtc
     4081 ccgctcggcc agttcgcggc tcgcgtcgag gtagctcatc gcaccgcgcg aaccggggtc
     4141 gtagtcgatg atcgtcatgc tgtacccggg cgcctcggaa accttcacgc tgcgcgggat
     4201 caccgtccgc aacaccttgt cgccgaagta acggcgcacc tccccagcca cctggtcggc
     4261 cagcttggtt cgcccgtcgt acatcgtgag gacgacggtc gagacggtga gcgcggggtt
     4321 caggtgcgcc ttgaccatct cgatgttgtt catcaactgg gacaccccct ccagcgcgta
     4381 gtactcgcac tggatcggga tcagtacttc cggagcggcg acgagcgcgt tgatggtgag
     4441 cagacccagc gatggcgggc agtcgatgaa gacgtactcg aaatcggtgc gatctaactc
     4501 cgcgagggcg gtgcgcaagc ggttttcgcg cgcgaccatg ctgaccaatt cgatctccgc
     4561 gccagccaga tcgatcgtgg cgggcacgca gaacagtcga tcactgtgcg ggcttcggcg
     4621 aagcgcgtcg gccagcttga tctctccgag aagtacctcg tacgacgacg gcgtgccggc
     4681 ctcccggtga tcgatcccca aagcggtgct cgcgttgccc tgggggtcca agtcgatcac
     4741 caacgtcttg agcccctgca gcgccagcgc ggcggccagg ttcactgcgg tcgtcgtctt
     4801 gccgacgcca cctttctggt tcgcgaccgt gaacagccgc cgatgagccg gccgcgggag
     4861 gctggggtga atcgtgtgca gcacatgcat cgcgcgctcg gcggccgcgc caatcggggt
     4921 gtcggtttca cgtgaaacat tcgccattgc gccccggtca gccgatgcct cacccggtgt
     4981 cacgatcgcc tcccactccg tgcccgcgct tgtgaggcct gcgcccgcga ggcggccgtc
     5041 cgctccgcga ccaccaccgt cgtcggcggc tccaaatagt ctccgccaca tctcaccacc
     5101 cttacagcct cggcgcctaa cgactccatc acacgccggt gttgcactac ttcttcgtct
     5161 gcgcggtcgc ccttgaccgc cagcaacaag cccccgtcgc gaagcagtgg gaggcaccac
     5221 tcggtcaact tgtcctgtgc cgcgaccgcc cggcacacca ccacgtcgac ctcaccgacc
     5281 cgctgccgca ccacgggatc ctctgctcgt ccccgaacga tctcgacatc gagccgcagc
     5341 tcgtcgacga cctcccgaag gaacgtcgtc cgccgcaaca tcggctcgat cagcgtcatc
     5401 cgcaagtccg gcctggcgag cgcgagcggt atgcccggga ggcccgctcc gcttccgacg
     5461 tctgccacac gcgcaccgtc ggcgatgaga ctctcgaccg cggcgctatt gaggaggtgc
     5521 cgttcccata cccgctcggc ctcgcgggga ccgatgagcc cgcgcttcac accgtcggtt
     5581 gcgagcatgc cggcataccg gaccgcgaga tctaagcggt caccgaacac cttctgcgcg
     5641 gccggtgggg ctcccggcgg ctccccatgt ttcacgtgaa acatcctccg ggcttcttca
     5701 aagggcctag gaccaccgcc gtcggaacta catcggtgta actcgggctg ctactggtcg
     5761 gcgagcacga caacgcggcg cgacggctct tggccctcgc tctcgctgcg cacgccggag
     5821 accgccgcca ccgcgtcatg gacgatcttg cgttcgaacg gcgtcatggg cgcgagctgt
     5881 tcacgtgtgc ccgtctccaa cacccgctgg gcgaccttgt gacctagcgc agccagttcg
     5941 tcacgccgct tccggcgcca gctggcgatg tcgagcatca accggctgcg ctccccggtc
     6001 ttctggtgca ccgccagccg ggtcagctcc tgcagggcgt caagcacctc cccgcgccgg
     6061 ccgaccagct tcaccagatc gtcgccgccg tcgatactga ccaccgcccg gttgccttcg
     6121 acgtcgaggt cgatatcgcc gtcaaagtcg agcacgtcca gcaactcctc gaggtagtcg
     6181 cccgcgatct ctccctcggt gactagccgc tcctcgagat cctcgggctt gggcgtctcc
     6241 gccgtcgcgt cgttcagctc gtcgtcgcgc tcagcggtgt ccgcgtcggt catccgtgcc
     6301 tctcccctct gtgcatctgc tcttcgcgca aagcctcatc gctctcaacg tttgcgtttc
     6361 ttcgggcgga cgcccggccg aggtgttcgg cccaccgagc cggcgccgtt cgctcgcgcc
     6421 gcattcggtt tcggctcctc gtccgtcggt ccgccaccgt ccacatccga cagctcgaca
     6481 cccgacgagt cggtgatctc ggcctcgtcc gacgaagccg cagccgactt cttcggctgc
     6541 cgtttgggct ttgcccccgg ggccggtgcg ttggccgcac gccgggcgat ggcctcctgc
     6601 ttcttctctt cttcctcgtg ggcgatcttt ccgaacacgt agtgctgctg tccgaacgtc
     6661 cagatgttgt tcgagaacca gtagatgatg atcgccagtg gcaggaacgg gccgccgacc
     6721 accacgccga gcggaaagat gtacagcgcc agccggttca tcatcgcggt ctgcgggttg
     6781 gccgccgcct ccggactctg gcgggcgatc gaggctcggc tgttcaggtg cgtcgcgaag
     6841 ccggccaaca gcatcatcgg taccccgatc gcgatcagtg ccggccggct gaagtcgaag
     6901 ttcttgaacg cttccaaacc cgacgtctgt gtcatgctgg cacccaacgg cgcgccgaac
     6961 aggttcgcgt cgaggaagtg ccccacgtcc gtcgggctga agacgtagtt gccgagactg
     7021 cgattcgtct ccgggtccaa tccaagccgc ccaatgccgt gctgcgtccg gttgaacgac
     7081 atcagcacgt ggtacagccc gaggaacacc ggaatctgcg cgagcatcgg caaacagccg
     7141 aggatggggt tcaccccgtg ttcccgctgc agcttctgca tctccagcgc gagccgctgg
     7201 cggtccttgc cgtacttctt ctgcagcgcc ttgatctgcg gctgcatctc ctgcatctgc
     7261 ctcgtggtac ggatctgccg gacgaatggc ttatacaaga tcgcgcgcag cgtgaacacg
     7321 aggaacatca ccgtcagcgc ccaagtgaag aagttggtgg gacccaacag gaacgcgaag
     7381 gccttgtacc agacccacat gatcgccgac accggataat agatgatgtc caggctgaat
     7441 ggatcaaaca caggacgtgg tctcccctcg ctgcgctggg gtatcccaga cgttgtcgac
     7501 ggcattaccg tctgctcttc ctgctcttcg cgcaagccgc tcatcgccgc gccggtgaag
     7561 ttcgttgtgc cggtcgggga ttgggtccca tcccccgcgg tgccacggcc cgcatttcgc
     7621 caaccgcacc gcagccaacc acccgcccct gaccaaaccg cattcggaca gcgcctccac
     7681 cgcgtactgg ctgcaggtcg gcatgaaccg gcacgtcggc aatcgcatgg gtgacacgta
     7741 cctccggtac aactcgatca cgaggatcac ggcccgcgcc ggcaaccgcc gcatcctcgt
     7801 cacagccgac gtcatcgttg ccccttcggc aaaacgtgcg ctcgggtcag acctgaccgc
     7861 agttcctgtg cgagtcgtgc cgagatcgcg tgtcgactgc tcggcagcgc tcggatgacg
     7921 atccggtcgg ctgggcccaa ttcggtcacg agggatagtg cgacatgccg cagccgacgc
     7981 gccactcgat gccgctgcac ggccgatccc actgattttc ctactaccaa gccaattcgg
     8041 ggacccggct cgccctgtcg gttacatcgc tgcgcgtgca cgacgagatc ccgttcgacg
     8101 gcccgcaccc cgttgttcac cgtggaaccg aactccgccg accgcgtcat ccggtatcgc
     8161 gccggaagca ccgtggtagc tatccgcgct gactcaggca gtcagcgtcc gccggccctt
     8221 gcgccgccga ttagatacga tcgcgcgccc ggcgcgggtc ctcatccgaa gccggaatcc
     8281 atgcactcgc gcacgtctgc ggttgttcgg ctggaaagtc cgcttgccct tggccacggc
     8341 attatctcct cgtgcgtctg gcacctcgcc gcctcgcgca tcgtctaact acgcatgccg
     8401 aagctgacac ggttgccgtt ggtaagctct tcgcctcgct cgctaatcgg cgcggacccc
     8461 gcgggtgcga tcccccggtt cgctgccgta tcgccgactt tcgggcgact gttcgagggt
     8521 actgacctga ataccaccgg tcaaacgacg ttccaccagc gcgcccagtt cactgccgtc
     8581 acagccgtca ccgttgtcaa gcgacacgcc gagttctggt cggcaatgtt acagaacggt
     8641 ggtcagccat gccgaaacct gttagcttct gccaatgccg tttccgtcgg agacggcccc
     8701 cacgacaagt catacacaac gaagtgaggg tgcctggtcg gttcctgatt catctcttcg
     8761 ccgagtcctc agcgcgccga ccgacaaccc accccgagcg acaacgaagc gagacgctgt
     8821 cttgtccaca cctgtggata actatgtgga cagctgtcat cgttttattt gtctaccctc
     8881 gaccgacgcc gaagggggtt ctcgtcgttg acagctgacc ccgacgcacc gttcgccgcg
     8941 gtatggaaca ccgtcgtctc cgaactcaac agcgatcaac ccgatggccc gtcgaccacc
     9001 aacgatgacc gcaccactcc cccgctcacg ccacagcagc gcgcctggct gactctcgtc
     9061 aaaccgctca ccatcgccga gggattcgcg ctgctgtcgg tgccgagcag cttcgtgcaa
     9121 aacgagatcg aacgacatct gcgaacacag atcgtcgacg cgctcagccg tcgtctcggc
     9181 caccgcgtcg aactcggcgt gcgcatcgcg cccccggagg gcggcgatgc cgacgatcta
     9241 ctggcaggac tgtcgcccac ctacgacacc gatctcgacg aggtcgacga cgacaccgac
     9301 gcgttggcca gtgcccacga atcctggccg tcgtacttcc tcaacaagcc gccccaggat
     9361 gccgggcaac cccaaggagt tagtctgaat aagcgctaca cgttcgacac gttcgtcatc
     9421 ggggcgtcga accggttcgc gcacgccgca gcgctcgcga tcgcagaagc gcctgcccgg
     9481 gcgtacaacc cgctgttcat ctggggcgag tcgggtctcg gcaagaccca cctgctgcac
     9541 gccgccggca actacgcgca gcgtctgttt ccggggatgc ggctcaagta cgtctcaacc
     9601 gaagagttca ccaacgactt catcaactcg cttcgtgacg accgcaaggt cgcgttcaag
     9661 cgcagctacc gcgacatcga cgttctgctc gtcgacgaca ttcagttcat cgaaggcaag
     9721 gaaggcatcc aagaggagtt cttccacacc ttcaacaccc tccacaacgc gaacaaacaa
     9781 atcgtcatct cgtcggaccg gccacccaag cagctcgcca ccctcgagga tcggctgcgc
     9841 acgcggttcg agtgggggct gatcaccgac gtccaaccgc ccgagttgga gacccgcatc
     9901 gcgatcctgc gcaagaaggc gcagatggac cggctcgacg cgcccgacga cgtactcgag
     9961 ctgatcgcca gcagcatcga acgcaacatc cgagagctcg agggcgcact catccgggtg
    10021 acggcgttcg cctcgctcaa ccaaaccccg atcgatcgtg cactggccga gatcgtcctg
    10081 cgtgacctca tctccgacgc cgaaagcatg cagatcagca cggcgatgat catggcggcc
    10141 accgcggagt acttcgaaac caacgtcgaa gaacttcgcg gacccagcaa gacgcgcccg
    10201 ctcgcgcagg cccgccaaat cgccatgtat ctctgccgag agctcaccga tctgtcactg
    10261 ccgaagatcg gccaggcctt cggccgtgac cacaccacag tgatctacgc agagcgcaag
    10321 attcgcggcg aaatggctca acgccgtgac gtcttcgatc aagtcaagga actgacgaca
    10381 cggatccgtc aacgttccaa gcgctgatct ctgattgcgc cccaaaaaac tttcgccacg
    10441 cctcccccag ccgtcacacg ttcacgtgtg cataaccctg tgcacaacat cagaacaaca
    10501 ccgccagctt catcatcgcc cattcacagc cggcagtcgt ccacacttcc cgcaccgaca
    10561 agacgccagc cgtccaccgc cgattcacac cccggcgcca cccgcttacg gcgcaaaagc
    10621 caggttgtcc acagattcca cagcgcctac tactgttgct taattatctc tctagatctc
    10681 agtcagaaga aggtcctggg gatacccctc gcccacaccg acctccacgt tggcgggcag
    10741 ccgttgtcgg ccagatggaa tacctttgat tgagcctacg aaagctctac ggtggtgcct
    10801 cgacagccat tacggtcgtc gtaggtgtgt gacggtcgca cgggtaaacc cgaagaaacc
    10861 gcggggacag agtgttgcga actgggatcg aagaagaggc tgtgaggtgg ctacgacgac
    10921 ggtcggtctg accgacttga aattccggct ggtccgcgag gacttcgccg acgcggtggc
    10981 gtgggtcgca cgtaacctgc cgacacggcc ggcggtgccg gtgctcgccg gtgtgcttct
    11041 agcgggcacc gacgcgggtt tgacgatctc cggtttcgac tacgaggtgt cgggcgaggt
    11101 tcaggtgcct gctgaaatcg caacgcccgg aaaggttttg gtgtcaggcc ggttgttgtc
    11161 cgacattacc cgggcgctgc cggccaagcc ggtggacgtc agcgtggacg gcactcgcgt
    11221 gtcattgacc tgcggtagcg cccgcttctc gctgcccacc atggccgtgg aggactaccc
    11281 gtcactaccc gcgctaccgg aggagacggg tgtcatctcc gctgacctgt tcaccgaggc
    11341 catcggccag gtggcggtgg cggccggacg cgacgacacg ttgccaatgc tcacgggcat
    11401 tcgggttgaa atctccggcg aatccgtggt tttggcggcc accgaccggt tccggctggc
    11461 ggtgcgtgag ctgacctggt cgacgacgtc agccgatatc gaagcggcgg tgctcgtgcc
    11521 ggccaggaca ctggccgaag ttgcgaaggc cggcatcacc ggagcagacg tcgcgctggc
    11581 gctcggtgcc gggcagaccg tcggcaagga ggggctgctc ggtatccgca gcgaaggcaa
    11641 gcgcagcacg accaggctcc tggatgccga gttcccgaag ttccgtcagc tgttgcctac
    11701 cgagcacacc gcggtcgcaa ctgttgaggt ggccgaactg agcgaagcga tcaaacgtgt
    11761 ggcgctggtt gcagaccgtg gtgctcaggt caggatggaa ttcagcgagg gcacactccg
    11821 gctggccgcc ggtgccgacg acgtaggccg ggctgaggag gacctgcctg tggagttctc
    11881 gggtgagccg ttgacgatcg cgttcaaccc gacctatctg accgacggct tgagctcatt
    11941 gaatagtgcg cgggtctcgt tcgggttcac cactcccagc cggccggcag tgctgcgacc
    12001 ggcgacggac gaggacggct caaacggcgg ttacggaccg ttcccggcgg tgcaatccga
    12061 ctacgtttac ctattgatgc cggtgcggtt acccggctga acagttcatt cgagcgaggg
    12121 aggcatcgat gcagctgggc ctggtaggtc tgggcaagat gggcttcaac atgcgcgaga
    12181 gattgcgcga tggcggccac gaggtggtcg gttacgatcc ccgtcccgaa gtcacggacg
    12241 tccccacgct ggccgcactg gccggcgcgc tcgctacgcc gcgggtggtg tgggtgatgg
    12301 tgccgtccgg gccggtcact aacgagacga tcgtggcgct ggccgccgag ctcggcgagg
    12361 gcgacttggt gatcgacggg ggtaactccc gctacaccga ggacgctccg cacgcaaaac
    12421 tgttggacga gaaaggaatt gcattcatcg atgccggtgt gtccggcggc gtgtgggggt
    12481 tgaaggaggg gtacgggctg atggtgggcg gcagcgaggc caacgtcgcc cgcgcaatgc
    12541 cgatcttcga cacgctgcgg cccccggggc cccgggagga cgggttcgtc cacgccggac
    12601 ccgtcggggc gggtcacttt gcgaagatgg tgcacaacgg cgtcgagtac gcgctgatga
    12661 ccgcttacgc cgagggctac gagatactga ccgccgagga cttgatccaa gacccgcagg
    12721 ccgtctacca ggcctggacc aacggcactg tggtgcggtc ctggcttcag cagctgctgg
    12781 ctaaagcgtt gaaggaagac ccgaacctgg ctgaaatcgc cggctacacc gaggattccg
    12841 gcgagggccg ctggactgtc gaagaagcga tccggttgcg ggtacccgtg ccgggcatct
    12901 ccgcgtcgtt gttcgcacgg ttcctgtccc gccaggacga ctcccccacc atgaaggccg
    12961 tcgccgcgct gcgcaaccaa ttcggcggac atgcggtgca ccgagtcagc cagtcgggat
    13021 agtccggccg ccgatgtatg tgcggcactt agggttacga gacttccggt cgtggccgca
    13081 ggcagacatc gagctggagc cggggcgcac cgtcttcgtc ggtccgaacg ggtacggcaa
    13141 gaccaatttg atcgaggcgt tgtggtattc ggcgacgctc ggctcgcatc gggtcgccac
    13201 ggatgcgccg ttggtccggg ccggcacctc gcgcgcggtg gtgtcgacga tcgtcgtcaa
    13261 cgacggccgg gaactggccg tcgacctcga aatcacgccc ggcaaggcca acaaggcgcg
    13321 gttgaaccgc tcccccgtcc gtagcccgcg cgaggtgctc ggagtgctgc gcgcggtgct
    13381 gttcgctccc gaggacctcg ccctggtgcg cggggacccc ggtgagcggc gccgctatct
    13441 cgacgagttg gcgacggtgc ggcggccggc gctcgccgcg gtgcgcgccg actatgaccg
    13501 cgtggttcgg cagcggaccg cgctgctgaa gacggcggcc gggcagcgcc cgcgcgatgc
    13561 cggattgctg caaaccctcg aggtgtggga cggccacctg gccgagcacg gggcacggct
    13621 gctggcggcg cgcgttgcgt tggtcaacga actgcgcccg gaagtggaaa aggcgtacca
    13681 gctgctggcc cccgggtctc gggctgccga catccgatac cggtcaagtg tggagttcga
    13741 cgcgggcggc gacggggact ctttcgagac ggcgctcctg gccgcgctag ccgcgcgacg
    13801 cgacgccgaa ctggaccgtg gcgtgtgcct ggtcggcccg caccgcgacg acttggagct
    13861 cgtgctcggc gaccagatcg cgaaaggctt tgcgtcacat ggcgagtcgt ggtccatggc
    13921 gttgtcgctg cggctagcgg cgtatgagct gttgcggtcc gagggcaccg atcctgtgct
    13981 actgctcgac gacgtgttcg cggaactcga caccgcgcgc cgccggtcgt tggcggtggt
    14041 cgcaggcgag gcagagcagg tcctgctgac ggcggcagtc cccgacgaca ttcccctcga
    14101 tc
//
LOCUS       DASWBF010000339         6297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_763018,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000339 DASWBF010000000
VERSION     DASWBF010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763018"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VF874_12510"
     CDS             <1..1030
                     /locus_tag="VF874_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9176852.1"
                     /translation="AAVSVPEIYERLDDIDAETFLRDINFPKAARHLAFEVFSRSFFA
                     EPTKLSAAELATMFHIYFLGSSEGLVFDVADANFDTCLWEPLRKYLSGMGVRFHTGVS
                     VTGIETGGSKTFRVHTDSVFDADAVVLATDVAGLQRIVDASSALGDETWRQKVRDLRT
                     APPFVVQRLWLDQPLAPDRPAFLGTGGLEPLDNISVLDRYERQAIDWARRHGGSVVEL
                     HSYAVRDPSDRVADRLRARLHELYPETRDAAVVAERILRRADCPRFAPGDYARRPGVV
                     TPHPSLMLAGDGIRIDLPVALMERAATTGWSAANQLLGGWGLAGHSLRTVPTQGRSAL
                     LRRLAESR"
     gene            1038..2006
                     /locus_tag="VF874_12515"
     CDS             1038..2006
                     /locus_tag="VF874_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5914 domain-containing protein"
                     /protein_id="HEX9176853.1"
                     /translation="MKWPKSSPIQILPRTDWAQQQPTYRDAEPAIIEAALQRSQRRPS
                     GNWYAFAASSSVRTNRPFGTSVAGVEIVAWRDEGGGLHVGPGTCPHLGADLATGTIDC
                     GTLICQWHGLRLSGKREFGWKPLPSHDDGVLVWARLDRVGGEEPLPQPVIPLRPSGFQ
                     LDTVTRLVGTCEPCDVIANRLDPWHGAWFHPYSFTRLEVLSAPAADASDDDDRFLVAV
                     TFKIGRFGVPVIAEFTSPEPRTIVMRIVDGEGSGSVVETHATPVGPGPRTAVIEAVIA
                     HSDRSGFAHTRRVAPLIAPLMRYAATRLWRDDLAYAERRYTVRSRR"
     gene            complement(2003..2377)
                     /locus_tag="VF874_12520"
     CDS             complement(2003..2377)
                     /locus_tag="VF874_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX9176854.1"
                     /translation="MSLRPRLQPSARHPITVTETGRHVTVRVNGEVVADTDNALTLQE
                     STYAAVQYIPLADVNGDVLRRTDTATYCPYKGDASYYSVVTASGVTVEDAIWTYEQPY
                     EAVAPIAEHVAFYPDKAEVTVG"
     gene            2415..2891
                     /locus_tag="VF874_12525"
     CDS             2415..2891
                     /locus_tag="VF874_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9176855.1"
                     /translation="MSQAEAPAAGANVTIDAAPDKIYALITDLPTLASLGDEIATMEW
                     HKGDAVQPGAVFKGHNRNGTKKWTTTCTVTDAEPGRVFAFDVRHTVLPIAHWRYDIEA
                     ADGGCRVTERTWDRRPAWFAKVAHLATGIKDRAAANTEHIQATLQRLKEKAESGNE"
     gene            2894..3292
                     /locus_tag="VF874_12530"
     CDS             2894..3292
                     /locus_tag="VF874_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX9176856.1"
                     /translation="MCRWVGYLGAPIAPKELLHDPHRSLIEQSRRHAPNMVIPNGDGT
                     GLGWYEHRGEPALFRSITPEWGDQNLLELAAEIRSPLFLAHVRAGTGTPVQRTNCHPF
                     RYRNWLFVHNGYVANYEQPRKPDSELRRCR"
     gene            3289..3564
                     /locus_tag="VF874_12535"
     CDS             3289..3564
                     /locus_tag="VF874_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176857.1"
                     /translation="MTVGVTDSRRIYAVRYASGGEVNTLFVSEDAFSVRMLYPENERL
                     AHFSDDTRVVVSEPLADLPGLWRAVPPGTALVIGDDVHEQSFEPVAP"
     gene            3625..4008
                     /locus_tag="VF874_12540"
     CDS             3625..4008
                     /locus_tag="VF874_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX9176858.1"
                     /translation="MWDTFWHGLWSLVMIFAFVAYLIILFNIIVDLFWRDHRSSGWVK
                     AIWVVFLILVPYLTAFVYLVARGQGMAVRAREAATAAKKETDAYIREAAGRSPAQEIA
                     DAKQLLDTGAITQQEFDSLKAKVLS"
     gene            complement(3986..5062)
                     /locus_tag="VF874_12545"
     CDS             complement(3986..5062)
                     /locus_tag="VF874_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEX9176859.1"
                     /translation="MRAFACPVCRGFAAFESVRCSNCGAELGFHLPSQAMIAVGEGCA
                     DIGGRRWVRCTKHGSPRCNWLVPEEQGEGYRGRCLADSLIRREPQPDDTIAVEKLVST
                     TVALRRLVFQLIDLGLPIDPFWRRDGGLAFDLLSSYTTGERVTIGHASGVITIDLVES
                     LDAYRESLRVHLGEPYRTMLGHFRHEVGHYYQNVLVETGDGASRYLDECRGLFGDERA
                     DYQAEIQRHYSFGPPKDWNQSYISEYATMHPWEDFAECFAHYLHITDTIDTAREAGLV
                     LRADKVPFAVPRDIVPLESYDDAPIELLLYDWKWLALLSNRVNSAMGKAPLYPFEIPP
                     PVVRKLGLVHRLIRETASNSAPLP"
     gene            5066..5521
                     /locus_tag="VF874_12550"
     CDS             5066..5521
                     /locus_tag="VF874_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HEX9176860.1"
                     /translation="MIVARSSTIEDYLDELRSTILEHGWAVQFVESDRTPYAYTIGLH
                     DCGLPELLLTGLTPQRAARLLNRVAEMTIRDGPPTPGDQLQVPAGPLVEAVTVDHPDA
                     HMDMAVAFYANPQLRALQLVWADRDGRWPWAAGFSDGRRRQPVLGKRAA"
     gene            complement(5518..>6297)
                     /locus_tag="VF874_12555"
     CDS             complement(5518..>6297)
                     /locus_tag="VF874_12555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9176861.1"
                     /translation="ERLGVTIYEQSPVTAISTGRAETLSGRVSAPVVVRATEGFTASL
                     RGLRRRWLPMNSSMIVTEPLPDDVWETIGWRECETVGDTAHGFFYAQRTVDDRIAIGG
                     RAVPYRYGSRIDRDGETDARTVNRLAATLHSALPQTRGARIAHGWCGVLGVPRDWNAS
                     VGFDPATGLGWAGGYVGHGVTATNLAGRTLADLVLLRRTPLTDLPWVNHRCRVWEPEP
                     LRWLGVRSMYWAYGLADRQESRGRSTTSPIALLADRIARRP"
BASE COUNT         1054 a         2141 c         2090 g         1012 t
ORIGIN      
        1 ggcggcggtg tcggtgcccg agatctacga gcggctcgac gacatcgacg ccgagacgtt
       61 cctgcgcgac atcaacttcc ccaaggcggc acggcatctg gcgttcgagg tgttctcccg
      121 cagcttcttc gccgagccga cgaaattgtc cgcggccgag ttggcgacga tgtttcacat
      181 ctacttcctc ggttccagcg aggggctggt gttcgacgtc gccgacgcga acttcgacac
      241 ttgcctgtgg gaaccgttgc ggaagtatct gtctggtatg ggtgtgcggt tccacaccgg
      301 agtgtccgtc actggcatcg agacgggtgg atccaaaacc tttcgggtgc acactgattc
      361 ggtttttgac gccgacgccg tggtgctggc taccgacgtg gcggggttgc agcgtatcgt
      421 cgatgcctcg tcggcccttg gcgacgagac gtggcgccag aaggtgcgcg acctgcggac
      481 cgcgccgccg ttcgtcgtgc agcggctgtg gctcgaccag ccgctggcgc cggaccgtcc
      541 ggcgttcctc ggcaccggcg gcctcgagcc gctcgacaac atcagcgtgt tggatcgcta
      601 tgagcgccaa gccatcgact gggcgcgacg gcacggcggt tcggtggtcg aactgcactc
      661 ctacgcggtg cgtgatccgt cggatcgggt ggccgaccgg cttcgcgccc ggctgcacga
      721 gctctacccg gagacccgcg acgccgccgt cgtcgccgag cggatcctgc gccgcgccga
      781 ctgcccccgc ttcgcccccg gtgactacgc ccgccgcccc ggtgttgtga caccgcatcc
      841 cagcctgatg cttgccggtg acggcatccg gatcgacctg ccggtggcgc tgatggaacg
      901 cgccgccacc accgggtggt cggcggccaa ccagttgctc ggcggctggg gtctggccgg
      961 gcactcgctg cgcaccgttc ccacgcaggg ccgcagcgca ctgctgcgcc ggctggccga
     1021 atcgaggtga cccacccatg aaatggccga aaagttctcc gattcaaata cttccgcgaa
     1081 cagattgggc acagcagcaa cccacctacc gcgacgccga accggcgatc atcgaggccg
     1141 cgttgcagcg ttcccagcgg cggccgtcgg gcaactggta cgccttcgcc gccagcagca
     1201 gcgtgcgcac gaaccggccg ttcggcacat cggtcgccgg tgtcgagatc gtcgcgtggc
     1261 gcgacgaggg cggcgggctg cacgtcggtc ccggcacgtg cccgcacctc ggcgcggatc
     1321 tggccaccgg aaccatcgat tgcggcacgc tcatctgcca gtggcacggt ctgcggctgt
     1381 ccggtaagcg cgagttcggc tggaagccgc tgccgagtca cgacgacggc gtgctggtgt
     1441 gggcgcggct ggaccgggtc ggcggcgaag aacccctgcc gcagccggtg atcccgttgc
     1501 gccccagcgg gtttcagctg gacacggtga cccgtttggt cggcacctgc gagccttgtg
     1561 acgtcatcgc gaaccggctg gacccatggc acggcgcatg gtttcatccg tactcgttca
     1621 cccggctgga ggtgctcagc gctccggccg ccgacgcatc cgatgacgac gaccgtttcc
     1681 tcgtcgcggt cacgttcaag atcggccgat tcggcgtacc agttatcgcc gagttcacca
     1741 gccccgagcc gcgcacgatc gtgatgcgga tcgtcgacgg cgagggctcc ggcagcgtcg
     1801 tcgaaaccca cgcgacgcca gtcggtcccg gaccgcgcac cgcggtgatc gaggcggtga
     1861 tcgcccactc cgaccggtcc gggttcgcgc acacccgccg cgtcgccccg ctgatcgcgc
     1921 ctctgatgcg gtacgcagcg acccggctct ggcgcgacga cctggcctac gccgaacgcc
     1981 gctacacggt ccgctcgcgg cgctagccga cggtgacctc ggccttgtcc gggtagaacg
     2041 cgacgtgctc ggcgatcggg gcgaccgcct cgtacggctg ctcgtaggtc cagatcgcgt
     2101 cctcgaccgt cacgccggag gcggtgacca cgctgtagta gctcgcgtca cccttatacg
     2161 ggcagtacgt cgcggtgtcg gtgcgccgca gcacgtcgcc gttgacgtcg gccaacggaa
     2221 tgtactggac ggcggcgtac gtcgactcct gcagcgtcag cgcattgtcg gtgtccgcca
     2281 cgacctcgcc gtttacgcgg acggtgacgt gtctgcccgt ttcggtgacc gtgatcgggt
     2341 ggcgggcact cggctggaga cggggtcgca ggctcatgag aaagtcaaca caacagccgg
     2401 cataagattc ccgcatgagc caagctgaag cacccgccgc cggtgcgaac gtcacgatcg
     2461 acgccgcccc ggacaagatc tacgcgttga tcaccgacct gccgacgctc gcgtcgctgg
     2521 gcgacgagat cgcgacgatg gagtggcaca agggcgacgc cgtgcagccc ggcgcggtgt
     2581 tcaagggcca caaccgcaac ggcaccaaga agtggacgac gacgtgcacg gtgaccgacg
     2641 ccgagcccgg ccgggtgttc gcgttcgacg tgcgccacac cgtcctgccg atcgcgcact
     2701 ggcgctacga catcgaggcc gccgacggag gctgccgggt caccgagcgg acctgggacc
     2761 gacggcccgc gtggttcgcg aaggtggccc acctggccac cggcatcaag gatcgggcgg
     2821 cggccaacac ggaacacatc caggcgacgc tgcagcggtt gaaggagaag gccgagagcg
     2881 ggaacgagta atcgtgtgcc ggtgggtcgg ctacttgggt gcaccgatcg ccccgaagga
     2941 actgctgcac gatccgcacc gatcactgat cgagcagagc cggcgacacg cgccgaacat
     3001 ggtgattccc aacggcgacg gcaccgggtt ggggtggtac gagcatcgcg gcgagcccgc
     3061 gctgtttcgc agcatcaccc cggagtgggg tgaccagaac ctgctggaat tggcggcgga
     3121 gatccgcagt ccgctgttct tggcgcacgt gcgggccggg acgggaacgc cggtacagcg
     3181 gaccaactgt catccgttcc ggtaccgcaa ctggttgttc gtgcacaacg gctacgtggc
     3241 caactacgag cagccgcgaa agccggactc agaactccgg cgctgcagat gaccgtcggc
     3301 gtcaccgaca gccgacggat ctatgccgtg cgctacgcca gcggcggcga agtcaacacc
     3361 ctgttcgtca gcgaagacgc cttctcggtg cggatgctgt atccggagaa cgagcggctg
     3421 gcccacttca gcgacgacac gcgcgtcgtg gtttccgaac cactggctga cctgccggga
     3481 ctatggcgag ccgtgccgcc gggcaccgcg ctggtcatcg gggacgacgt ccacgaacag
     3541 agcttcgagc ccgtcgcgcc atagccgcgc gtgcactata ttgacggagc cgaaaacgac
     3601 gacaacgccg aggagaagct gaagatgtgg gatacgttct ggcacggact ctggtcgctg
     3661 gtgatgatct ttgcgttcgt cgcctacctg atcatcctgt tcaacatcat cgtcgacctg
     3721 ttctggcgcg atcaccggtc ctccgggtgg gtcaaggcga tctgggtcgt attcctgatc
     3781 ctcgtcccgt acctcacggc gttcgtgtat ctggtcgccc ggggtcaggg catggccgtg
     3841 cgcgcccggg aagccgccac ggccgccaag aaggaaaccg atgcctacat ccgcgaagcg
     3901 gccggccgct cacccgcgca ggagatcgcc gacgcgaaac agttgcttga taccggcgct
     3961 atcacccagc aggagttcga ctctctcaag gcaaaggtgc tgagttagaa gcggtctcgc
     4021 ggatcaaccg gtggacgagg ccgagtttgc gtaccacggg cggcgggatt tcgaacgggt
     4081 atagcggcgc cttacccatc gcgctgttga cccgattgga caacagcgcc aaccacttcc
     4141 agtcgtagag cagcagctcg atgggtgcgt cgtcatagct ctcgagcggc acgatgtcgc
     4201 gcggcaccgc gaaggggacc ttgtcggccc gcagcaccag ccccgcctcg cgggccgtgt
     4261 cgatggtgtc ggtgatgtgc aggtagtggg cgaagcactc ggcgaagtcc tcccacggat
     4321 gcatcgtcgc gtactccgaa atgtaggact ggttccaatc cttcggcggc ccaaaggagt
     4381 aatggcgctg aatctcggcc tggtagtcgg ctcgctcgtc gccgaacaga ccgcggcact
     4441 cgtcgagata tcggctcgcg ccgtcgccgg tctcgactaa gacgttctgg tagtagtggc
     4501 cgacctcatg ccggaaatgg cccagcatgg tgcggtacgg ctcgcccagg tgtacccgca
     4561 gcgactcccg gtaggcgtcc agtgactcga ccaggtcgat cgtgatcact cctgacgcgt
     4621 gcccgatggt cacccgctca ccggtggtgt agctggacag gagatcaaag gccaacccac
     4681 catcgcgccg ccagaacggg tcaatcggca aaccaaggtc gatcagctga aacaccaagc
     4741 gccgcaacgc cactgtggtc gatacgagtt tctccacggc gatcgtgtcg tcgggttggg
     4801 gttcccggcg gatcagcgaa tccgccaaac accggccacg gtagccctcg ccctgctcct
     4861 caggcaccaa ccagttgcag cgcgggctgc cgtgcttggt gcaccggacc cagcgccgac
     4921 ccccgatgtc ggcacacccc tcaccgacgg cgatcatcgc ctgggacggc agatggaacc
     4981 caagctccgc tccgcaattc gagcaacgca ccgactcgaa cgcggcaaag ccccggcaca
     5041 ccggacaggc gaatgcgcgc attcgttgat cgtcgcacgc tccagcacca tcgaggatta
     5101 cctcgacgag ctccgctcca caatcctcga gcacggttgg gctgtgcagt tcgtcgagag
     5161 cgaccgaacg ccgtacgcgt acacgattgg gctccacgac tgcggactgc ccgaactctt
     5221 gctgaccggg ctgaccccgc agcgggccgc acgcttgctg aacagagtcg ccgaaatgac
     5281 cattcgcgac gggccgccga cgccggggga ccaactccaa gtgcccgccg gcccgctcgt
     5341 cgaagcggtg acagtggacc atcctgatgc gcacatggac atggccgtgg cgttctatgc
     5401 caacccccaa ttgcgcgcac ttcagcttgt gtgggctgac agggatggcc gatggccatg
     5461 ggctgccggc ttcagtgacg gccgacgacg gcaaccggtg ctcggcaagc gggccgccta
     5521 agggcgtcgc gcgatccggt cggccagaag cgcgatcggc gaagtcgtcg accggccccg
     5581 gctttcctgc cggtcggcca ggccgtaggc ccagtacatg ctgcgcacac ccagccaccg
     5641 cagcggctcg ggttcccaaa cgcggcagcg gtggttgacc cacggcaagt cggtgagcgg
     5701 cgtgcgacga agcagcacca aatcggccag cgtccggcca gccaggttcg tcgcggtcac
     5761 gccgtgcccg acgtagccgc ccgcccagcc gaggccggtc gcggggtcga atccgacgct
     5821 ggcgttccag tcccgcggca cgccgagcac tccgcaccag ccgtgcgcaa tgcgcgcgcc
     5881 gcgggtctgc ggaagcgcgc tgtgcagcgt cgccgcaaga cgattcacgg tgcgggcgtc
     5941 ggtttctccg tcgcggtcga tccgcgagcc gtagcggtac ggcaccgcgc gaccgccgat
     6001 cgcgatgcgg tcgtcgaccg tgcgctgggc gtagaagaac ccgtgcgcgg tgtcgcctac
     6061 ggtctcgcat tcccgccagc cgatcgtctc ccacacatcg tccggcagcg gttcggtgac
     6121 gatcatcgag ctgttcatcg gcagccatcg tcgccgcaac ccgcgcaacg acgcggtgaa
     6181 gccctcggtg gcgcgcacga cgaccggtgc gctgacgcgg ccggaaagcg tttcggcgcg
     6241 tcccgtgctg atcgcggtga cggggctctg ttcgtagatc gtcactccca gccgctc
//
LOCUS       DASWBF010000340         7735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1219748,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000340 DASWBF010000000
VERSION     DASWBF010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219748"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VF874_12560"
     CDS             complement(<1..319)
                     /locus_tag="VF874_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5642 family protein"
                     /protein_id="HEX9176862.1"
                     /translation="MTAATACSAPVHTPPPSPSSPAAAQAKQVNPANIRRMRGSFPPG
                     YEVSEIEGVASPATYWGLKPGWSAEPAECGVLADPVAGSPQGLSGSGSGGIVYVAVAR
                     APSA"
     gene            459..770
                     /locus_tag="VF874_12565"
     CDS             459..770
                     /locus_tag="VF874_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176863.1"
                     /translation="MRAASTLAAAVIATGAALAAPAGFASADNGAQTTINYWEQQGYQ
                     VNIDRVGSGPINECVVTSVRNPNTITRFVRVGGKNDNNPSFLVPVIVSRTVQVSLDCS
                     G"
     gene            complement(793..1776)
                     /locus_tag="VF874_12570"
     CDS             complement(793..1776)
                     /locus_tag="VF874_12570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9176864.1"
                     /translation="MTVVAADLGSLPVFAGIPTDELSALAAELTPIHAPVGEVLMRQG
                     EEALSFFLIASGEVEVRHTDSDGQTLVAFLSEGLIGEIALLRNAPRTATVVATDDVFG
                     YVGNEEAFHELLQIPGIAPQLVRTARQRLAAFITPIPFEARDGTDLMLRPVLPGDTQR
                     VAHGHVMFSPETLYRRFLSVRTPTATVLTYLFEVDYVDHFVWVVTDGEDGPVVADGRF
                     VRDQDDPISAEIAFTVADEYQGRGIGSMLFTALAVAARVDGITRFHARVLADNLPARA
                     LLDRLDAPWIREEPGVVAATIDVPEPEKLPGKINHLKEIRHVARQVIRAFE"
     gene            1915..3513
                     /locus_tag="VF874_12575"
     CDS             1915..3513
                     /locus_tag="VF874_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9176865.1"
                     /translation="MLRRLVTRVGDEGWSVLQLARAGLLRPVSPVQLAAILRAVRDYG
                     ELGAAVTLAVVRHRDAVGLIDERGALTFGDLDARSNAVANEWRRAGLQPGSTVAILAR
                     NHRGLLDALFAGMKTGARVVLLNTDFGRTQISDVIAREGADLLVYDEEYADAVADVDV
                     RHGCVRAGTDRPGSDTLDEQIRRGDPSPPLAPGVKPSIVILTSGTTGTPKGASRSVPR
                     SLLPLAGLLDKAPFRTGEVTVCCAPMFHSLGFGHLMIAMILGSTLVVRRRFDPEWALE
                     SIARHRASALIVVPIMLRRILDLGPQARMDSALRIIFVAGSQLGAELCTRALEAFGPV
                     VYNLYGSTEVAYGTVATPEDLAEEPGCVGRLVRGAVVKIFDDDGHQVPTGTTGRILVG
                     NSMQFEGYTGGGTKETIRGLMSSGDVGHFDSAGRLFIDGRDDEMIVSGGENVFPCEVE
                     DVLASHPDVLDAAVIGAPDDEYGQRLRGFVVVRSNASLSEQDVKDYVKSRLARFKVPR
                     DVVFVDELPRNPTGKVVKRLLDAE"
     gene            complement(3520..3951)
                     /locus_tag="VF874_12580"
     CDS             complement(3520..3951)
                     /locus_tag="VF874_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9176866.1"
                     /translation="MKIVDANVLLYSVNSESEHHRASLQWLDNALSGADGVGFAWMPL
                     LAFLRLTTREGLFPRPLHPEEAIGQVAEWLGAPTATAVVPTARHFDVLARMIEQVGTG
                     GNLVNDAHLAALAAEHRASIVSYDSDFGRFGVRWHRPDELL"
     gene            complement(3948..4202)
                     /locus_tag="VF874_12585"
     CDS             complement(3948..4202)
                     /locus_tag="VF874_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEX9176867.1"
                     /translation="MRTTVTLDDDVVQLVRKRMAEEQVSFKKALNDAIREGAVGRHAP
                     RRFATRVVDMGVPAINLDRALQIAADLEDEELIRRQRRRA"
     gene            4291..4815
                     /locus_tag="VF874_12590"
     CDS             4291..4815
                     /locus_tag="VF874_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176868.1"
                     /translation="MLARSAVAAVALLCGAFAFIGPAQAADPVLDGTYRLDFDGANRT
                     INGAPSPIANTSATYSFTSSCAENGCVARAILLSSNDTEAVSAHNPDLTLQFVDGTWQ
                     LSLPYDSPCEEGDRNQLLTWSLTPQSGAGVLSGIRTVATVGQSCAGDDAGPLAQPMTA
                     TRVGSAAPGVLPMP"
     gene            4889..5773
                     /locus_tag="VF874_12595"
     CDS             4889..5773
                     /locus_tag="VF874_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HEX9176869.1"
                     /translation="MNAEQHHKMAQDAGFVAALDQSGGSTPKALRLYGIEEDEYSGDE
                     EMFELVHQMRARIITSPSFDGNRILAAILFENTMDRDIEGRPTADYLWDVKNVVPILK
                     VDKGLADEQDGAKVMKPMPNLDDLLARAVDKGIFGTKMRSVVTLPGKGLDAVVDQQFE
                     VARQILAAGLVPIIEPEVDIKSPQKAEAEDQLKACLLDGVNQLGPDQTVMLKLTLPDV
                     DNLYRELVEHPKVLRVVALSGGYSRDEACARLARNNGVIASFSRALTEGLTAQQSAQE
                     FDAMLDKSIAEIAAASAT"
     gene            complement(5766..6665)
                     /locus_tag="VF874_12600"
     CDS             complement(5766..6665)
                     /locus_tag="VF874_12600"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9176870.1"
                     /translation="MDIGFVGLGNMGFPIARRLLDAHYDLVVYDTRPDAVETLVELGA
                     RAASSTREVADRAETVMASLPSPQASFDVATGAGGVIDGSRVKRFVDFSTVGSQVAER
                     INATLVERGIATLDSPVSGGVAGAQKGTLAVMVSGPRTDFDVLTPVYETIGRPFYVGE
                     KPGSAQTMKLVNNMLAGTALVATCEAVVMGVKSGLDPAVMIDVLNAGSGGTTASRDKF
                     PRSILPRTFDFGFATGLMVKDVRLYLDEARALGVSTEVADAVGRLWEVVMTELGPDSD
                     FTSAIKPIERAAGVVVGGEAGVR"
     gene            complement(6672..7054)
                     /locus_tag="VF874_12605"
                     /pseudo
     CDS             complement(6672..7054)
                     /locus_tag="VF874_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            7207..7455
                     /locus_tag="VF874_12610"
     CDS             7207..7455
                     /locus_tag="VF874_12610"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9176871.1"
                     /translation="MPGIFDDASMAATIVRVRDVAASVRWYRDKLGLEPIYVGDDGPD
                     HPVAAYSIAGVVVSLWQLPAGHSGCRRTRTATATWSWS"
     gene            7452..7637
                     /locus_tag="VF874_12615"
     CDS             7452..7637
                     /locus_tag="VF874_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176872.1"
                     /translation="MTGDLEPARRALLERGIDVGEIRRSANNEFLWFHDLDGNRFELS
                     RPLIAQEEGTGEATNGP"
BASE COUNT         1331 a         2553 c         2566 g         1285 t
ORIGIN      
        1 tcgccgacgg ggcgcgggcc accgccacgt agacaatgcc cccgctaccg gatccggaca
       61 gtccttgcgg gctgccggcc accgggtccg cgagcacacc gcattccgcc ggctccgcag
      121 accaaccggg tttcagtccc cagtacgtcg ccggtgaggc gacgccctcg atctccgaga
      181 cctcgtaacc aggcgggaag gatccgcgca tgcgccggat attggccggg ttgacctgct
      241 tcgcctgagc tgccgcaggc gacgacggac tcggcggcgg ggtgtgcacc ggcgcggaac
      301 aggcggtggc ggccgtcact gccagcaggg tgaactggcg cagacccacg cccgttgata
      361 ccacagagat tctttggcaa ttccggaaca ttcgggcgcc caaaagcgat gtactcgatg
      421 agtactccca ctggttcggg gatgaaggag gcggggctgt gcgcgcagca tcgaccctcg
      481 ccgcagcggt aatagcgacg ggcgccgccc tggccgcccc ggccgggttc gcgtcagccg
      541 acaacggggc gcagaccacc atcaactact gggaacaaca gggctaccaa gtcaacatcg
      601 accgggtagg tagcggcccg atcaacgaat gcgtcgtcac gagcgttcgc aacccgaaca
      661 caatcacccg gttcgtccgg gtcggcggaa agaatgacaa caatccgagc ttcctggtcc
      721 cagtgatcgt gagccgcacc gtgcaggtgt cgctggactg ttccggctga gccgcctcgc
      781 ggcaggtggc cgtcattcga acgcccggat cacctggcgg gcgacgtgcc gaatctcctt
      841 caggtgattg atcttgcccg gcagcttctc cggttccggc acgtcgatcg tcgcggccac
      901 gacgccgggc tcctcgcgaa tccacggcgc gtcgagccga tccagcaggg cgcgggcggg
      961 cagattgtcg gcgagcaccc tggcgtggaa tcgggtgatg ccgtcgactc gcgcggcgac
     1021 cgcaagcgcc gtgaacagca tcgagccgat gccgcggccc tgatactcgt cggccacggt
     1081 gaacgcgatc tccgcgctga tcgggtcgtc ctggtcgcgc acgaagcggc cgtccgcgac
     1141 cactgggccg tcctcgccgt cagtgaccac ccagacgaag tggtcgacgt agtccacctc
     1201 gaagaggtac gtcagcacgg tcgcggtcgg cgtccgcacc gacaggaaac gccggtacag
     1261 cgtctccggc gagaacatca catgcccgtg cgccacccgc tgtgtgtcgc ctggcagcac
     1321 cggtcgcaac atcaggtcgg tgccgtcgcg ggcctcgaac ggaatgggcg tgatgaaagc
     1381 ggccagccgc tggcgggccg ttcggaccag ttgaggggcg atgccgggga tctgcagcag
     1441 ctcgtggaac gcctcctcgt ttccgacgta gccgaacacg tcgtcagtcg cgacgacggt
     1501 tgcggtgcgc ggagcattgc gcagtagagc gatctcgccg atcaggccct cggacaagaa
     1561 cgcgacaagg gtctggccgt cgctgtcggt gtgccgcacc tcgacttcgc cggacgcgat
     1621 gaggaaaaag gacagggctt cttcgccttg gcgcatcagc acctcgccga ccggggcatg
     1681 aatcggtgtc agttcggcgg ccagcgctga cagctcgtca gtggggatcc cggcgaacac
     1741 cggaagtgag ccgaggtccg ctgcgacgac ggtcatcggg ttcgttcctt gcggcagctg
     1801 atcagccgac gatatttgcc gaaaaatttc ttgtcgagtg ttagcactca cacctcggtt
     1861 catgccgatg gacctcgaca aggtcgacga ggttcttttg gcatgctcgt gagcatgctg
     1921 cggcgcctcg tcacgcgggt cggcgacgaa ggctggtcgg tgctgcaact ggcgcgcgcc
     1981 ggcctgctgc gccccgtgtc cccggttcag ctggccgcga tcctgcgtgc ggtccgcgac
     2041 tacggcgaac tcggcgcggc ggtgacgctg gcggtggtcc gccaccgcga cgccgtcggg
     2101 ctcatcgacg aacgcggagc cctgacgttc ggcgacctcg acgcccggtc caacgcggtg
     2161 gccaacgagt ggcgccgcgc cggactgcag ccggggtcca ccgtcgcgat cctggcgcgc
     2221 aaccatcgtg gactgctcga cgcgttgttc gcgggcatga agaccggcgc gcgagttgtg
     2281 ttgctgaaca ccgacttcgg ccgcactcag atcagcgacg tgattgcgcg ggagggcgcc
     2341 gacctgctgg tgtacgacga ggaatacgcc gacgcagtcg ccgatgtcga cgtgcgccac
     2401 ggttgtgtgc gcgcggggac cgatcggcca ggctcggaca ccctcgacga gcagatccgg
     2461 cgtggcgatc cgtcaccgcc gctggcgccc ggcgtgaaac cgagcatcgt gatcttgacg
     2521 agcgggacga cggggacacc gaagggcgct tcgaggtcgg ttccgcggtc gctactgccg
     2581 ctggccggct tgctcgacaa ggcgccgttt cgcaccggtg aagtcacggt gtgctgcgcc
     2641 ccgatgtttc actcgcttgg gttcggccac ttgatgattg cgatgatctt ggggtccacg
     2701 ctcgtggtgc gtcggcggtt cgatcccgag tgggcgctgg aaagcattgc gcggcatcgt
     2761 gcttcggcgt tgatcgtggt gccgatcatg ttgcgccgca ttctcgatct cgggccgcag
     2821 gcccgcatgg attccgcgct acggatcatt ttcgttgccg gctcgcagct cggcgcggag
     2881 ctgtgcaccc gtgcgctgga ggcgttcggg ccggtggtct acaacctcta cggctcaacg
     2941 gaagtcgcgt atggcacggt cgccacgccc gaggatctcg ccgaggagcc cggttgcgtc
     3001 gggcggctgg tccgcggagc tgtggtcaag attttcgacg acgacggcca ccaggtgccg
     3061 accggcacga cgggccgaat ccttgtcggc aacagcatgc agttcgaggg ctacaccggc
     3121 ggcgggacga aggagacgat ccgcggcctg atgagcagcg gcgacgtggg ccacttcgac
     3181 tctgccggac ggcttttcat cgatggccgc gatgacgaga tgatcgtctc cggtggagag
     3241 aacgtgtttc cctgtgaggt ggaggacgtg ctcgcttcgc acccagacgt tctcgacgcg
     3301 gcggtgatcg gagcacccga cgacgaatac gggcagcggc tgcgcgggtt cgtcgtcgtg
     3361 cggtcgaacg cgtcgctgtc cgagcaggac gtcaaggact acgtcaagag ccggctcgcc
     3421 aggttcaaag tgccgcgaga cgtcgtgttc gtcgacgagc taccgcgcaa cccgacgggc
     3481 aaggtggtca agcgtctgct tgacgccgaa tgacggtgcc tacaacagct cgtcggggcg
     3541 gtgccaccgg acgccgaacc ggccaaagtc gctgtcgtag ctgacgatgc ttgcgcgatg
     3601 ctcggcggcg agcgccgcga ggtgggcatc gttgaccagg tttccgccgg ttcctacctg
     3661 ttcgatcatc ctcgccagga cgtcgaagtg ccgagcggtg ggcactacag ctgtggcggt
     3721 cggagcgccg agccactccg cgacttgccc aatcgcctcc tccggatgca atggacgagg
     3781 aaacaaaccc tctctggtcg tcaacctcag gaacgcgagc agcggcatcc atgcgaaccc
     3841 gacaccgtcg gcgcccgaca gcgcgttgtc gagccattgg agagacgcac gatggtgttc
     3901 gctttccgaa ttcaccgagt acaggagcac attggcgtcg acgatcttca tgctcgtcga
     3961 cgctggcggc ggatgagctc ttcgtcttcg aggtcggccg cgatctgcag agcccggtcg
     4021 agattgatcg ccggcacgcc catatcgacg acacgcgttg cgaatctacg tggtgcgtgc
     4081 cggccaacag caccctcgcg gatggcgtcg ttgagcgcct tcttgaacga cacctgctct
     4141 tctgccatgc gttttcggac cagttgaacg acgtcatcgt cgagggtcac agtggtccgc
     4201 atattgacag catagcatca agtaatgtga tgctatgctg tcaaatccga acccaccgcg
     4261 tcgacgctcc ggcgagcgta ggttcgacgg atgctcgctc gctcagccgt ggcagccgta
     4321 gcccttctct gcggcgcctt tgcgttcatc ggccccgccc aggccgccga ccccgtcctc
     4381 gacggcacct accggctcga cttcgacggt gcgaatcgga ccatcaacgg cgcaccgagc
     4441 ccgattgcca acacatcggc gacgtactcc ttcacgtcct cgtgcgccga aaacggctgc
     4501 gtcgcacgcg ccatcctgct gagctccaac gacacagagg ccgtctcagc gcacaacccc
     4561 gacctgaccc tgcagttcgt cgacggcacg tggcagctgt ccctgccata cgactcgccc
     4621 tgcgaagagg gtgacaggaa tcagctgctg acgtggtcgc tcaccccgca aagcggggcc
     4681 ggcgtgctct cggggatccg cacggtggcg acggtcgggc agtcatgcgc cggggacgac
     4741 gcgggtccgc tagcccagcc catgaccgcg acccgcgtcg gcagtgcggc cccgggcgtc
     4801 ctaccgatgc cctaacctcg cccgcgccga tgcactatgt aaacatgcac acgaggggca
     4861 caggcgcccc ggacggcggg aggtagtgat gaacgcagaa cagcaccaca agatggccca
     4921 ggacgccggc ttcgtcgccg cactggatca aagtggcggc agcacgccga aggcgcttcg
     4981 gctgtacggc atcgaggagg acgagtactc cggcgacgag gagatgttcg agctggtgca
     5041 ccagatgcgc gcccgcatca tcaccagccc cagcttcgac gggaaccgga tcctcgcggc
     5101 gatcctgttc gagaacacga tggaccgcga tatcgagggc cgcccgaccg ctgattacct
     5161 gtgggacgtc aagaacgttg ttccgatcct caaggtcgac aagggcctgg ccgacgagca
     5221 ggacggcgcc aaggtgatga agccgatgcc gaatctcgac gacctgttgg cccgggcggt
     5281 cgacaagggc atcttcggca ccaagatgcg ctcggtcgtc acgctgccgg gcaaggggct
     5341 cgacgcggtg gtcgaccagc agttcgaggt cgcccggcag atcctggccg ccggcctcgt
     5401 gccgatcatc gagccggaag tggatatcaa gagcccgcag aaggccgagg ccgaagatca
     5461 actcaaggcc tgcctgctcg acggcgtcaa ccagctcggc cccgatcaga ccgtcatgtt
     5521 gaagctgacg ctgcccgacg tcgacaactt gtatcgggag ctcgtcgagc atccgaaggt
     5581 gctgcgcgtt gtcgcattgt ccggcggcta cagccgcgac gaggcgtgtg cccggcttgc
     5641 ccgcaacaac ggcgtgatcg cgagtttctc ccgggcgctg acggaggggc tgacggcaca
     5701 gcagagtgcg caggagttcg acgcgatgct ggataagtcg atcgccgaga ttgcagcggc
     5761 gtcggctacc taacaccggc ctctccgccg acgaccacgc ctgccgcccg ctcgatcggc
     5821 ttgatcgccg aggtgaagtc cgagtcgggc ccgagttcgg tcatcacgac ctcccacagc
     5881 cgccctactg cgtcggccac ctccgtcgag actccgagcg ctcgtgcctc gtccagatag
     5941 agccgcacgt ctttgaccat caagcccgtg gcgaagccga agtcgaaggt gcgcggcagg
     6001 attgaccgcg ggaacttgtc gcggctcgcg gtggtgccgc ccgacccggc gttgagaacg
     6061 tcgatcatca ccgccgggtc caggccggat ttgacgccca tcacgacggc ttcacaggtg
     6121 gcgacgagag cggtgcccgc gagcatgttg ttgaccagct tcatcgtctg ggccgaaccg
     6181 ggcttctccc cgacgtagaa cggcctgccg attgtctcgt acaccggcgt cagcacgtcg
     6241 aaatccgttc ggggaccgga caccatcacc gccagggtgc ccttctgcgc gccggccacc
     6301 ccgccgctga cgggactgtc gagggtggcg atgccgcgtt cgacgagcgt tgcgttgatc
     6361 cgttctgcca cctggcttcc cactgtggag aagtcgacga accgcttgac ccgtgagccg
     6421 tcgatgacgc cccctgcgcc ggtggcgacg tcgaaggacg cctgcgggga tggaaggctc
     6481 gccatcaccg tttcggcgcg gtcggccacc tccctggtcg acgacgcggc acgcgcgccg
     6541 agctcgacga gagtctcgac ggcgtcgggc cgggtgtcat acaccaccag gtcgtagtgc
     6601 gcgtcgagca gccgccgcgc gatcggaaaa cccatgttcc ccagaccgac aaacccgatg
     6661 tccatggcgt cttaccctcg atcgagttcg gccaacactt cccgcgcgac gcggaagcta
     6721 tcgactgcag cggggacgcc cgcatagacg gcgacttgca tgaagacctc tcggatctcc
     6781 tctcgcgtca caccgttggt cagagccgcg cggatgtgcg tacgcaactc ctgcggacgg
     6841 ttgaggacgg cgatcatggc caaggtcacc atgctgcggg tcttgcggtt cagctcctca
     6901 cggggcccca caccgcgccc cagcagtatt cggtgccgag gtcctggaac ggcttggtga
     6961 ggctgtccgc gtccgcggcc gctttcttga cgtactccgc gcccagcacg tccgtgcgga
     7021 tctcgaggcc cttttcgaaa gtattgtcag ccaatgatcc tcctcatctc aggacagggc
     7081 caccgcgagc ggttcgcagt tgactctgcg tccagggcgt gaaagttcga gtaggccgcg
     7141 ccctcagcgc agaatgaacg caccaagcgt cgcacaccgg gctccggtca ggcctactgt
     7201 cagacgatgc cgggcatctt cgacgacgcg tcgatggctg cgaccatcgt gcgggtacgc
     7261 gacgtggccg cgtcggtgcg ctggtatcgc gacaagctcg ggttggaacc gatctatgtg
     7321 ggcgatgacg ggccagacca ccccgtcgcc gcctactcga tcgcaggggt cgtcgtgtcg
     7381 ttgtggcagc tgcccgccgg gcacagcggg tgccgcagga caaggaccgc aacagctacg
     7441 tggtcgtggt catgacgggc gacctcgaac cggctaggcg agcgctgctc gagcgcggga
     7501 tcgacgtcgg cgagattcgt cgcagcgcga acaacgagtt cctgtggttc cacgacctcg
     7561 acggcaaccg cttcgagctg agccgaccgc tgatagcgca agaggaagga accggggagg
     7621 ccaccaatgg accgtgatca gctgatcgac ctcacccgcc gcgcgctcaa gttggcgcgc
     7681 gacaagacca ccgaccttgc gcccgcacga agaccgtgat gggtcgctcg atcct
//
LOCUS       DASWBF010000341         9114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_763321,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000341 DASWBF010000000
VERSION     DASWBF010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763321"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VF874_12620"
     CDS             <1..616
                     /locus_tag="VF874_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9176873.1"
                     /translation="LVDVVGLYMNPPEKAIVLCADEKSSVQALDRTQASLPMVKGRGE
                     TMTHDYKRHGTTTLFAALDVLTGTVIGQCMPRHRHQEWLKFLKTIDREVPKDLAIHLI
                     LDNYATHKHQDVGTWLDNHPRFHLHFTPTSSSWLNLVERWFRELTDKALRRGAFHSVP
                     DLITSIQEYIDSHNDAPQPYVWTATAESILAKVARGRIALEKVS"
     gene            complement(729..1634)
                     /locus_tag="VF874_12625"
     CDS             complement(729..1634)
                     /locus_tag="VF874_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX9176874.1"
                     /translation="MQKLRTPDERFADLPEFPYVPSYSDIDDGEGGTLRVAWVSDGTD
                     TADPVLLLHGEPSWSFLYRKMIPVLVAAGHRVICPDLVGFGRSDKPTLHEDHTYARHV
                     EWMRALAFDVLNLNRVTLVGQDWGGLIGLRLAAEHPERFAGIVVANTGLPTGDFPMPD
                     IWWRFREAIQGLPMIDVGRFVQSGCVRPMTDDVRAGYDAPFPDDAYCAGPRAMPGLVP
                     TAPDDPASAANRAAWAALCESPTPMLVAFSDKDPITGAMADIFRRQMRGAQGRQHPVV
                     RDAGHFLQEDAGVELANHIVEFLKT"
     gene            1784..2233
                     /locus_tag="VF874_12630"
     CDS             1784..2233
                     /locus_tag="VF874_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HEX9176875.1"
                     /translation="MKRGFVVALGGAAIVVAGLAGCSSNKASEGNNTAAAGKGSAKLT
                     IDGKDQTISGDIGCTTTGDTVSIALGDQSKGLVGATVKGNEVETVGIIIDGQPLAYAK
                     GTPNNEAKLTKDGSKYMIAGSLTGMPDMSNPMAGPSKHDFTMDVTCP"
     gene            complement(2297..2779)
                     /locus_tag="VF874_12635"
     CDS             complement(2297..2779)
                     /locus_tag="VF874_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9176876.1"
                     /translation="MPRHSAGLLPYRVRDDVVEVLIGHPGGPFWAKKDDGAWSILKGE
                     YVDGEDPWEVAQREFTEETGLAPPAGPRIDFEPVRQPSGKVLTAFAVHGDADISDAHS
                     NTFEIEWPKGSGRMREFPEIDRVQWFPVGQARMKLLKGHLAILDRLMAHPAVAELREG
                     "
     gene            complement(2783..4144)
                     /locus_tag="VF874_12640"
     CDS             complement(2783..4144)
                     /locus_tag="VF874_12640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9176877.1"
                     /translation="MKLISPIDNIFLLGESREHPMHVGGLQLFAPPDGAGPDFVREVY
                     EAMLSATDVQPTFRKHPAFLGGITNIAWSSDKDIDLEYHLRRSALPRPGRVRELLELT
                     SRLHGSLLDRHRPLWETHLIEGLDDGRYALYCKMHHALIDGVSAQRLTQRVLSPDPND
                     EELRVPWALKPPRRKGSGRGPSPLQRVTSTAGSIAALAPSTLGLVRHGLLEQQLTLPF
                     QAPKTMFNVRIGGARRVAAQSWSLERFKRVKAAAGGVTVNDVILAMCAGALRAYLLEH
                     SALPETPLVAMVPVSLRSDKDHDRGGNMVGTVLCNLATHLDDPAKRLDAIHASMAENK
                     KVFTQLPQIEQFALSAFLSGGVALALVPGFVSNAPPPFNIVISNVPGPREPHYWNGAR
                     LDGNYPLSIALDGQALNITMTNNADNLDFGLVGCRASVPHLQRLLGHLETSLKDLERA
                     AGV"
     gene            4253..5230
                     /locus_tag="VF874_12645"
     CDS             4253..5230
                     /locus_tag="VF874_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9176878.1"
                     /translation="MGLTVAVTGPTGEIGISAVTALEREPAVEKIVGMARRPFDPTAH
                     GWTKTTYQRGDILDRDAVDGLVADADVVIHLAFIIMGSRDESARINLQGTRNVFEATV
                     AAPRPRRLVYTSSVAAYGYHSDNPVPITEDVPTRGSPEHYYSQQKAACEAALAEITDG
                     SSLEVFILRPCIVAGPHAPALADAMPWNKLPALLRAVSKALPLWKPVVPDPGFPLQLV
                     HHDDVAAAIALAATAPAPPGAYNIAGEGLVTLSDVGKALGGRPVRVPAVAASAALAVL
                     SRLPFVPSALEWLHVARTSVVMDTSKAKKDLGWQPTYTSAETLAALATR"
     gene            complement(5265..5492)
                     /locus_tag="VF874_12650"
     CDS             complement(5265..5492)
                     /locus_tag="VF874_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176879.1"
                     /translation="MSATNDRQALSEIAEQSGWQRRQVDRTDFYRRGAREVEVLFTED
                     KLNGGSLYEDLRMMTYTRDKATIESWLTRAV"
     gene            complement(5565..5966)
                     /locus_tag="VF874_12655"
     CDS             complement(5565..5966)
                     /locus_tag="VF874_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176880.1"
                     /translation="MAARSKSTVVVTVLLVYGIYSTLLGLVMIVAPGFFFDTVGGFGP
                     RNGHYIFDTAAFELPLGLLYLAAIRWPSWRVPVLAFATAHYVLHAISHLIDVNTANIH
                     WVGVFDFVVIVVGLVIHALALWFSVQLRQSD"
     gene            complement(6016..6243)
                     /locus_tag="VF874_12660"
     CDS             complement(6016..6243)
                     /locus_tag="VF874_12660"
                     /inference="COORDINATES: protein motif:HMM:NF023742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9176881.1"
                     /translation="MFRMRPTVRQHIALRACVEAHRELYNAALQERRDGWSHNKTRIS
                     YGDQSSHRASADQMAARNILRAGLALHAQAA"
     gene            complement(6255..6473)
                     /locus_tag="VF874_12665"
     CDS             complement(6255..6473)
                     /locus_tag="VF874_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9176882.1"
                     /translation="MSGRRFRRTAGGMSSPRLYVVWCAKYRRRVLGGCVARGCGELLQ
                     QIADEHGWQVVATEVSRDLEHQWDAVAS"
     gene            6614..8488
                     /locus_tag="VF874_12670"
     CDS             6614..8488
                     /locus_tag="VF874_12670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019931886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9176883.1"
                     /translation="MSERERGAPGHVGPEVLTEHTAPERLVVARGLFAGPSPRVPDDL
                     YAQVVRGTARRERNALHLEQGATVDTDTYFGRLPASYFQRWTTVTEVQLKLAFDTSGP
                     AQLRLRGSDAQGSARTVASTEIDGTGTAALSARLNEYFDGGALWMDCTALSGPLKITD
                     LEWTVAAPATIRPTAIAICTFNRADECAATVAAIAGDKTLLAQIDAVYVTDQGTDAVA
                     TRPLFNDVAAQLGDKLVYLRQPNLGGAGGFTRGLYEVSSIADHANVILMDDDILCEPE
                     TVLRLNAFANLTPTPTCVGAQMLLLKNPRKLLTSGEEIELAKIRNRWVANALHYSDMV
                     KNRQEKRVDAQYNAWWSCLIPSEVITAIGLPIPLFFQWDDIEYGLRALAAGFPTVTLP
                     NAGVWHADFHWKDRDDFVRYFSVRNPLITHALHGDIDTRVTGRWLAREIAHHLVSMQY
                     GLAHTVIRGIEDFLEGPEILRDGGVAALARIRKERSEYPETVIQPAGKIAELTGAVPR
                     VRTRGYLPRKDRLDLVLIKRTVYQWLGRTIPGPVAITSEDNVWWHVALFDHVVVTDAS
                     QAGVRIRRRDKRKLVKLTRRAVKALRRFRAEAPTLQDQYRAALPELTSRENWARLFES
                     "
     gene            complement(8567..>9114)
                     /locus_tag="VF874_12675"
     CDS             complement(8567..>9114)
                     /locus_tag="VF874_12675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9176884.1"
                     /translation="KAPVFWHPHDTLRFALGEIKGPGLALEFGVATGTTLSIIADTVA
                     GDREVFGFDSFNGLPETWRTGFEAGEFAQEPPHDIPGATVVTGLFEDSLSAFLAEHDE
                     PIAFLHLDADLYSSTKTVLDLVGDQLAPDAVLLFDEFFNYPGWRRHEYRAFNEFIART
                     GRTFEYLGYTGNNEQVALRLH"
BASE COUNT         1633 a         2985 c         2990 g         1506 t
ORIGIN      
        1 actggtcgat gtcgtcgggc tgtacatgaa cccgcccgag aaggcgatcg tgttatgcgc
       61 ggacgagaag tcctcggtgc aggcattgga ccgcacgcag gcgtcgctgc cgatggtcaa
      121 ggggcgaggc gagacgatga ctcacgacta caagcggcac ggcaccacca ccctgttcgc
      181 tgccctggac gtgttgaccg gcacggtgat cgggcaatgc atgccgcgcc accggcatca
      241 ggagtggttg aagttcctca agaccatcga ccgcgaggtg cccaaagacc tagcgatcca
      301 cctgatcctg gacaactacg ccacccacaa acaccaagac gtcggcacgt ggctggacaa
      361 tcatcctcgg tttcacctgc atttcacccc gacatcctca tcctggttga acctggtgga
      421 acgctggttt cgcgagctga ccgacaaggc gttgcggcgc ggggcatttc attccgttcc
      481 cgacctgatc acctcgatcc aggaatacat cgactcacac aacgacgccc cacagcctta
      541 cgtgtggaca gcgaccgcgg aatccatcct cgccaaggtc gcccgcggac gcatcgccct
      601 cgaaaaagtc agctaaatcc aggacacacc actaggtctt gccaacacga ctctgcggcg
      661 cacaccctaa gcggcgtgtc gtgtccgaat tcgcagtgtc gcgagcaggg tgaggtcgcg
      721 aggggcggct acgtcttcag gaactcgacg atgtggttcg caagttcgac tcccgcgtct
      781 tcctgcagga aatggccggc gtcgcggacg actgggtgct gccggccttg ggcaccgcgc
      841 atctggcgtc ggaagatgtc tgccatcgct cccgtgatgg ggtccttgtc gctgaacgcg
      901 accagcatcg gggtggggct ttcgcagagc gcggcccagg cagcccggtt ggcggccgac
      961 gccgggtcgt cgggagcggt cggcacgagg ccgggcatcg cgcgcgggcc ggcacagtag
     1021 gcgtcgtcgg gaaacggcgc gtcgtagccg gcacgcacgt cgtcggtcat cgggcgaacg
     1081 caccccgact gcacgaaccg gccgacgtcg atcatgggca gtccctggat cgcctcgcgg
     1141 aatcgccacc agatgtcggg catcgggaag tccccggtcg gaagaccggt gttggcgacg
     1201 acgatgccgg cgaatcgctc gggatgctca gcggccaggc gcagcccgat cagcccgccc
     1261 cagtcctggc ccaccagggt gacccggttg aggttcagca cgtcgaatgc caacgcccgc
     1321 atccattcga catgtcgggc gtaagtgtgg tcctcgtgaa gggtgggctt gtccgaccga
     1381 ccgaacccga cgagatccgg gcagatcacc cgatgaccgg cggcgacgag aacaggaatc
     1441 atcttgcggt acaggaatga ccacgacggc tctccgtgca gcagcaacac cgggtcggcg
     1501 gtgtcggtgc cgtcagaaac ccacgcgacg cgcagagtgc ctccctcgcc gtcgtcgatg
     1561 tcggaatagc ttggcacgta gggaaattcg ggtagatctg cgaacctctc gtcgggcgtg
     1621 cgcagtttct gcatgcagga gaacttaccg ccgcgccgaa ggccgggctg cggccatgac
     1681 gagatttttc acgtaaacgc gtgactgccg gggaagccgc caccatactg cttgacgatg
     1741 gtccgccgat cttgacgggt cacgacaaca aaaggagaac agtgtgaagc gtggattcgt
     1801 ggtcgccctc ggcggcgcgg cgattgtggt ggcagggctg gctggctgtt cgagtaacaa
     1861 agcgagcgaa ggcaataata cggctgcggc cggcaagggc tcggcgaaac tcaccatcga
     1921 cggcaaggac cagaccatct cgggtgacat cggttgcacg acgacgggag acactgtctc
     1981 catcgcgctt ggtgaccagt cgaagggcct cgtcggcgcg acggtcaagg gaaacgaggt
     2041 ggaaacggtc ggcatcatca tcgacggaca gccgctcgct tacgccaagg gcacacctaa
     2101 caacgaagcg aaactgacca aggacggaag caagtacatg atcgcgggca gtttgacagg
     2161 aatgcccgac atgtccaacc cgatggctgg cccttcgaag cacgacttca ccatggacgt
     2221 gacgtgcccg tagcgtaggc actcgagcga aagcggcggc actccgaggg gcgtcgccgc
     2281 ttttgtgtgc gggacgctac ccctcccgca gttcggccac ggccggatgc gccatcagcc
     2341 gatcgagaat cgcaaggtgg cctttcagca gcttcatacg cgcttggccc accggaaacc
     2401 attgcacgcg atcgatttcc gggaactcac gcattcgtcc cgaccccttg ggccactcga
     2461 tctcaaaggt gttgctgtgc gcgtcgctga tgtcggcgtc gccgtgcacg gcgaacgcgg
     2521 tcagcacctt tccgctcggt tgccgaaccg gctcgaagtc gatgcgcggg cccgcaggcg
     2581 gcgcgagacc ggtttcctcg gtgaactccc gctgggcgac ctcccacggg tcctcgccgt
     2641 cgacgtactc ccccttcagg atcgaccacg ccccgtcgtc tttcttggcc cagaacggtc
     2701 cgccgggatg cccgatcaga acctcgacga cgtcgtcgcg gacccggtac ggcagcaggc
     2761 ccgcgctgtg cctaggcatg cgtcagacgc cggcggcgcg ttccaggtcc ttcagcgatg
     2821 tctcgaggtg gccgaggaga cgctgcaggt gcggcacgct ggcccggcag ccgactaggc
     2881 cgaagtcgag gttgtcggcg ttgttggtca tggtgatgtt caacgcttgc ccgtccaggg
     2941 cgatcgacaa cgggtagttg ccgtctaacc gcgcgccatt ccagtagtgg ggttcgcgcg
     3001 gaccggggac gttggagatc acgatgttga acggcggcgg cgcgttggag acgaatcccg
     3061 gcacgagcgc cagcgccacc cctccactca agaaggccga cagtgcgaac tgctcgatct
     3121 gcggcagttg ggtgaacacc ttcttgttct cggccatcga cgcgtggatg gcgtcgaggc
     3181 gcttcgcggg atcgtcgagg tgcgtggcga gattgcacaa gaccgtcccg accatgttgc
     3241 cgccgcggtc gtggtccttg tcgctccgca ggctgacggg gaccatcgcc accagcggtg
     3301 tctccggcag cgcgctgtgc tcgagcaggt aggcacgaag cgctccggcg cacatcgcga
     3361 ggatcacatc gttgacggtc accccgccgg cggcggcctt cacccgcttg aagcgctcca
     3421 gcgaccacga ctgcgccgcg acgcgccgcg caccgccgat gcgcacgttg aacatcgtct
     3481 tgggcgcctg gaacggcagc gtgagctgct gctcgagcag cccatggcgc accagcccaa
     3541 gtgtcgaggg agcaagtgct gcaatcgatc ccgcggtgct tgtcacccgc tgcaacggcg
     3601 acgggccgcg gccggaaccc ttgcggcggg gcggcttgag cgcccacggc acgcgcagtt
     3661 cttcgtcgtt ggggtcggga ctcaacacgc gctgcgtcag acgctgcgcc gagacaccgt
     3721 cgatcagcgc atggtgcatc ttgcagtaca gcgcgtagcg tccgtcgtcg aggccttcga
     3781 tcagatgcgt ctcccacagc gggcgatgtc ggtcgagcag gctgccgtgc agccgcgagg
     3841 tcagctccag cagctcgcgt acgcggcctg gcctcggcag cgccgagcgc cgcaggtggt
     3901 actcgaggtc gatgtccttg tcggacgacc aggcgatgtt ggtgatcccg ccgaggaacg
     3961 ccggatgctt gcgaaacgtc ggctgcacgt cggtggcaga cagcatcgcc tcgtagacct
     4021 cgcgcacgaa atccgggccg gcgccgtcgg gcggcgcgaa cagctgcaga ccacccacat
     4081 gcatgggatg ttcgcgcgac tcaccgagca ggaagatgtt gtcaattggt gaaatcagct
     4141 tcatctgcat tccccgtccc aagagtcggt ctccacaacc tttgtaaccg cgtccagcgt
     4201 acgtttgacc gtacgaggtt tcgcggctag tgacgacagg caggattggc gagtgggact
     4261 gaccgtggcc gtcaccggcc ctacgggcga aatcggtata tcggccgtga cggcactgga
     4321 gcgagagccg gcggtagaga agatcgtcgg gatggcgcgc cggccgttcg acccgacggc
     4381 acacggctgg acgaagacga cgtatcagcg gggcgacatt ctcgaccgcg acgctgtcga
     4441 cgggctggtc gccgacgccg acgtggtgat ccacctggcg ttcatcatca tggggtcgcg
     4501 cgacgaaagc gctcgcatca acctgcaggg cacccgcaac gtgttcgagg cgaccgtcgc
     4561 cgcgccgcgg ccgcggcgac tggtgtacac gtcgtcggtc gccgcgtacg gctaccactc
     4621 cgacaacccg gtgccgatca ccgaggacgt gccgacgcgc gggtcgccgg aacactacta
     4681 ctcgcagcag aaggcggcgt gtgaggcggc actcgccgag atcaccgacg ggtcgtcgtt
     4741 ggaggtgttc atcctgcggc cgtgcatcgt cgccgggccc cacgcgcccg cgttggccga
     4801 tgcgatgccg tggaacaagt tgcccgccct gctgcgcgcg gtctctaaag cgctgcccct
     4861 gtggaagccc gtggttccgg atccgggctt tccgctgcag ctcgtccacc acgacgacgt
     4921 cgccgcggcg atcgcgttgg cggccaccgc tcccgcgccg ccgggtgcgt acaacatcgc
     4981 gggcgaaggg ctcgtcaccc tgtcggatgt ggggaaagcg ttgggcggca ggcccgttcg
     5041 agtgccggcg gtggcagcgt cggcggcatt ggcggtgctg tcccgcctgc ccttcgtgcc
     5101 gtcggcgctc gagtggctgc acgtcgcgcg cacgtcggtg gtgatggaca cctcgaaggc
     5161 caagaaggac ctgggctggc aacccacgta cacgtcggcg gagacgctgg ctgcgctggc
     5221 cacgcgatga gccgcttgcg cgaagagcaa aaggccgcgg ccgtttagac cgcccgggtc
     5281 agccagcttt caatggtcgc cttgtcgcgg gtgtacgtca tcatgcggag atcctcgtac
     5341 agcgagccgc cgttcagttt gtcctcggtg aacaggacct ccacttcccg ggcgcctcgc
     5401 cggtagaagt cggtgcggtc gacttgtctg cgctgccatc ccgactgctc ggcgatctcg
     5461 gacagcgcct gacgatcgtt cgtcgcgctc atgggggtgt cctcctgact gtgactctgc
     5521 gcaggctata gcagggccga gaattggccg gcgcggagac cggttcaatc cgactggcga
     5581 agctggacgc tgaaccacag cgcgagcgcg tggatcacca gtccgaccac gatgacgacg
     5641 aagtcgaaca cgccgaccca gtggatgttc gcggtgttga cgtcgatcag gtggctgatc
     5701 gcgtgcagga cgtaatgcgc cgtcgcgaac gccaacacag gcacccgcca acttggccac
     5761 cggatcgcgg cgaggtagag cagtcccaac ggcagttcga acgccgcggt gtcgaatatg
     5821 tagtggccgt tgcgcgggcc gaagccgccg acggtgtcga agaagaatcc gggcgcgacg
     5881 atcatcacca gcccgagcag tgtcgagtag atcccgtaga ccagcagcac cgtgacgacg
     5941 acggtcgact tcgaccgagc agccatggtg cttacactgt gacttctccg acggctggaa
     6001 gccgccggct tcttttcacg ccgcttgcgc atgaagggcc agtccagccc ggaggatgtt
     6061 gcgtgcggcc atctggtctg ccgacgcgcg atgcgacgac tggtccccat agctgatgcg
     6121 ggtcttattg tgcgaccagc catctcgacg ttcctgcagc gcagcgttgt agagttcgcg
     6181 gtgcgcctcc acacacgccc gcaacgcgat gtgctggcgc acggtcggac gcatccgaaa
     6241 catataagcc cgcctcacga cgccaccgca tcccactgat gctcgaggtc gcggctgacc
     6301 tccgtggcca cgacctgcca gccgtgctca tcggcaatct gctgcagcag ttccccgcac
     6361 ccgcgggcga cgcaaccgcc cagaacccgg cggcgatact tcgcgcacca caccacgtac
     6421 agccttggcg acgacatccc tcccgcagtg cggcgaaacc ggcgaccgga catacccgca
     6481 ccctacgaga cgggaccgac aacccccgcg atccgacgct gtgcgctccg atcaccccac
     6541 cggccgtttc gtggccggtc cgggccgggc ggagttgtta gtctcagaca gaccacgacg
     6601 acttaggagc agcatgagcg agcgcgaacg gggcgcgccg ggtcatgtcg gccccgaagt
     6661 gctcactgag cacaccgcac ccgagcgatt ggtggtggcc cgtggcttgt tcgccggccc
     6721 gagcccccgg gtcccggatg acctgtatgc gcaggtcgtg cgcggcacgg cccggcgcga
     6781 acggaacgct ctgcacctcg agcagggcgc gaccgtcgac accgacacct acttcggccg
     6841 gctgcctgcc agctacttcc agaggtggac caccgtcacg gaggtgcagc tcaagctggc
     6901 attcgacacg tcgggcccgg cccagctgcg gttgcgcggg tcggacgcac aaggttccgc
     6961 ccggacggtc gcgagcacgg agatcgacgg caccgggacg gccgcgttgt cagcccgact
     7021 caacgagtat ttcgacggcg gcgcgctgtg gatggattgc acggcgcttt ccggtccctt
     7081 gaagatcacc gatctggagt ggacggtggc cgcccctgcg acgatccgcc cgaccgccat
     7141 tgccatctgc acgttcaacc gggccgacga atgcgcggcc accgtcgcgg cgatcgccgg
     7201 cgacaagacc ctgctggcgc agatcgacgc ggtgtacgtg accgaccagg gcaccgacgc
     7261 cgtcgccacg cgaccgctgt tcaacgacgt cgccgcgcaa ctcggcgaca aactggtgta
     7321 cctccggcag ccgaatctcg gtggggcagg cggcttcacg cgcgggctct acgaggtgtc
     7381 gagtattgcc gaccacgcca acgtgatcct gatggacgac gacatcctct gcgagcccga
     7441 aaccgtgttg cggctcaacg cctttgccaa cctgacgccg acaccgacgt gcgttggcgc
     7501 tcagatgctg ttgctgaaga acccccgaaa gctgctcacc agcggcgaag agatcgagtt
     7561 ggcaaagatt cgcaaccgat gggtggcgaa cgcgcttcat tactccgaca tggtcaagaa
     7621 ccggcaggag aagcgcgtcg acgcccaata caacgcctgg tggtcgtgtc tgatcccgtc
     7681 cgaggtgatc accgcgatcg gcctgccgat cccgctgttc ttccagtggg acgacatcga
     7741 atacgggctg cgggcgctcg ccgcgggctt tcccaccgtc acgctgccca acgcgggcgt
     7801 ctggcatgcc gacttccact ggaaggaccg cgacgacttc gtgcgctact tctccgtgcg
     7861 caacccactg atcacgcacg cgctgcatgg cgacatcgat acccgcgtga cgggcagatg
     7921 gttggcgcgc gagatcgccc accacctcgt ttcgatgcaa tatggcctcg cgcacacggt
     7981 gatccgcgga atcgaagact tcctggaagg gcctgagatc ctccgtgatg gcggggtcgc
     8041 ggcgctggcc aggatccgca aggagcgctc ggagtatccg gagaccgtca tccagccggc
     8101 cgggaagatc gccgagctca ctggtgcggt gccgcgagtg cgcacgcgcg gttatctacc
     8161 gcgtaaggac aggttggacc tggtgctgat caagcgcacg gtgtaccagt ggctcggacg
     8221 aaccatcccc gggccggtcg cgatcacctc cgaagacaac gtgtggtggc acgttgcgct
     8281 gttcgaccac gtcgtcgtga ccgatgcctc gcaggccggt gtccggatcc ggcggcgcga
     8341 caagcgaaag ctggtgaagc tcaccagacg agcggtcaag gcgctgcgac gattccgcgc
     8401 tgaggcgccg acgctgcagg accagtaccg cgccgcactt ccggagttga ccagtcggga
     8461 gaactgggcg cggctgttcg agagctgatt cgtcgaaagt gacgttttgc aggccccctc
     8521 tcgcacttct cctgcgaaac gtcgatttcg ttgtgccaca tgatgatcag tgcagcctca
     8581 gggccacctg ttcgttgtta ccggtgtacc cgagatattc gaacgtgcgc ccggtgcgcg
     8641 cgatgaactc gttgaaagcc cgatactcgt ggcgccgcca tcctgggtaa ttgaagaact
     8701 cgtcgaacag cagcaccgcg tcgggcgcga gctggtcgcc gaccagatcc agcacagtct
     8761 tggtcgagga gtacaggtcc gcgtccagat gcaggaacgc gatcggttcg tcgtgctcgg
     8821 cgaggaaggc ggacaacgag tcttcgaaca aacccgtcac caccgtggca cccgggatgt
     8881 cgtgcggcgg ttcctgggcg aattcgccgg cctcgaagcc ggttcgccac gtctccggca
     8941 gaccattgaa gctgtcgaat ccgaagacct cgcggtcacc ggccaccgta tcagcgatga
     9001 tcgagagcgt cgtgccggtc gccactccga attcgagtgc caagccgggg cccttgatct
     9061 cgccgagagc gaatcgcagc gtgtcgtgcg gatgccagaa caccggcgcc ttgg
//
LOCUS       DASWBF010000342         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_763358,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000342 DASWBF010000000
VERSION     DASWBF010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763358"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VF874_12680"
     CDS             <1..144
                     /locus_tag="VF874_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9176885.1"
                     /translation="RLPVGEDFALRARSSIGPDGVGVTSAEIFDRSGFIGVCAQTLLV
                     QRR"
     gene            274..807
                     /locus_tag="VF874_12685"
     CDS             274..807
                     /locus_tag="VF874_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9176886.1"
                     /translation="MRNDPLPKAVVVGIDGSREAILAATWAADEAIARDVSLRLVYVN
                     PADSPGHGFRTAMAELALRVAAKAVAEQEKPVKVETVLLTGPAAKGYAAKVLIRESRY
                     ADVLCLGAVGMGRLATTVLGSTATALTRRGKCPVAIVSKPMRSEEPIAVVVDPSDDGP
                     AAATAASSPAGGSAPAR"
     gene            complement(761..2128)
                     /locus_tag="VF874_12690"
     CDS             complement(761..2128)
                     /locus_tag="VF874_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEX9176887.1"
                     /translation="MTQRFDAIVIGAGQAGPPLAGRLTDAGQTVAVLERKHIGGTCVN
                     TGCIPTKTLVASAHAAHLARRGADYGVGTGSVTVDMAKVKARKDGIMLNDRAGVESWL
                     EGMKGCTVIRGHARFEDPHTLRVDDELISADRIFLNVGGRAVVPDIPGLDGVEYMTNV
                     SILELDRLPEHLVIIGGSYIALEFAQMYRRFGADVTVVQRGSRLAAREDPDVSAAIKD
                     ILEGEGIEVILGANAIRVARDGDGVAVTPRDGADPMVGSHLLMAVGRQPNTDDLGLDR
                     AGVDTDKRGFIVVDDQLRTNVEHIWAMGDCNGRGAFTHTSYNDFEIVAANLLDNDPRR
                     VSDRIPTYALYIDPPLGRAGQTVTQVRESGCNALVGFRPMTRVGRAVEKGETQGFMKV
                     VVDADSHEILGAAIFGVGGDEVIHAILDVMSAKVPYDTLARTMHIHPTVSELVPTLLQ
                     EMTPL"
     gene            complement(2125..2607)
                     /locus_tag="VF874_12695"
     CDS             complement(2125..2607)
                     /locus_tag="VF874_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HEX9176888.1"
                     /translation="MTHVAVIVLALLIGVVAGLRALTAPAVVSWAAFLGWFDLDNRWL
                     HWVSHPAAVTVFTVLAVIELLTDPLPKTPSRKTGPAFGARLVAGGFAGAVLGSSFHYT
                     WSSVGAGVIGAVLGTLGGYEVRKRLVAAIGGRDWPIALLEDVVAVGGGFLIAYLASTL
                     "
     gene            complement(2634..>2918)
                     /locus_tag="VF874_12700"
     CDS             complement(2634..>2918)
                     /locus_tag="VF874_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX9176889.1"
                     /translation="QTAAARDQLAARVQGVVFGQQYLMLDYDCPRVVLDQAAAITPGL
                     ESPTIAPLADPDWVAVRALVPRKDVNTIMDELAGIGAKAILASDIRFCRF"
BASE COUNT          503 a         1002 c          944 g          469 t
ORIGIN      
        1 cggctgcctg tcggcgagga cttcgcgctg cgggcacgca gctcgatcgg ccccgacggg
       61 gtcggcgtga cctccgcgga gatcttcgac cgctccgggt tcatcggtgt ctgcgcacag
      121 acgttgctgg tgcagaggcg ctagccggca ccagcaaaca agaggccatt gccggagact
      181 gagggaccaa agaccccatc ggcccgacca cctggatcga taacgtctcc gacacggcaa
      241 gcagacggtt tgccagggaa cggagacaca gcaatgcgaa acgacccgtt gccaaaggcg
      301 gtcgtcgtcg gcatcgacgg ctcgcgggag gcgatcctgg cagccacatg ggccgccgac
      361 gaggcgatcg ctcgcgatgt ttcgctgcgg ctggtttacg tcaatcccgc ggattcgccc
      421 ggccacggct ttcgcactgc gatggcagag ctggcgctgc gcgtagcggc gaaagctgtc
      481 gccgaacaag agaaacccgt caaggtggaa accgtgttgc tgacgggtcc cgcggcgaag
      541 ggctacgcgg cgaaggttct gattcgcgag tcgcggtacg ccgacgtgct gtgcctgggc
      601 gcggtcggca tggggcggct ggctaccacg gtgttgggtt cgacggccac ggcgctgacc
      661 cggcgcggca aatgcccggt agcgattgtc tccaagccga tgaggtctga ggagcccatc
      721 gcggtcgtgg tcgacccgtc ggacgacggc cccgctgccg ctacagcggc gtcatctcct
      781 gcaggagggt cggcaccagc tcgctgacgg tcggatggat gtgcatggtg cgcgcgaggg
      841 tgtcgtaggg caccttggcc gacatcacgt ccagaatcgc gtggatcacc tcgtcgcccc
      901 cgacgccgaa gatcgccgca ccgaggatct cgtggctgtc ggcatcgacg accaccttca
      961 tgaacccttg cgtctcgccc ttttccaccg ctcggcccac ccgcgtcatc ggtcgaaacc
     1021 cgaccagcgc gttgcatcct gactcgcgaa cctgggtgac ggtctgcccg gcccgtccca
     1081 gtggcggatc gatatagagc gcatacgtcg gaatccggtc gctgacccgg cgcgggtcgt
     1141 tgtcgagcag gttcgcggcc acgatctcga agtcgttgta cgacgtgtgg gtgaacgcgc
     1201 cgcgaccgtt gcaatctccc atcgcccaga tgtgctcgac gttggtgcgc aattggtcgt
     1261 ccaccacgat gaacccgcgc ttgtcggtgt ccacccctgc gcggtccaga cccaagtcgt
     1321 ccgtgttggg ctgacgaccg accgccatca gcaggtgcga gccgaccatc ggatccgcgc
     1381 catcgcgggg tgtcaccgca acgccgtcgc cgtctcgcgc gacgcgaatg gcgttggcgc
     1441 ccaagatcac ctcgatacct tcaccctcga ggatgtcttt gatcgccgca gacacatcgg
     1501 gatcctcgcg tgccgccagc cgtgacccgc gctgcaccac cgtcacgtcg gcgccgaacc
     1561 gccggtacat ttgggcgaat tccagggcga tgtagcttcc gccgatgatc acgagatgct
     1621 ctggtagccg gtcgagttcg aggatcgaca cattggtcat gtactcgacg ccgtcaagtc
     1681 ccgggatgtc cggcacgacg gcgcgcccac cgacgttgag aaagatcctg tcggcggaga
     1741 tcagttcgtc gtcgacccgc aacgtgtgcg ggtcttcgaa gcgggcgtgc ccgcggatga
     1801 ccgtgcagcc cttcatgccc tccagccacg actcgacgcc cgcgcggtcg ttcagcatga
     1861 tgccgtcctt gcgggccttg acctttgcca tgtcgacggt gacggaaccg gtgccgacgc
     1921 cgtagtcggc accgcgccgg gccaaatgcg ccgcgtgtgc gctggccacc agcgtcttgg
     1981 tcgggatgca cccggtgttg acgcaggttc cgccaatgtg cttgcgctcc agcaccgcaa
     2041 cggtctgacc ggcgtcggtc agccgcccgg ccagcggcgg acccgcctgt cctgcgccga
     2101 tgacgatcgc gtcgaaccgt tgcgtcacaa cgtgcttgcc agataggcga tcaagaagcc
     2161 gccgccgacg gcgaccacgt cctcgagaag cgcgatcggc caatcgcgcc cgcctatcgc
     2221 ggccaccagg cgctttcgca cctcatagcc gccgagcgtg ccgagcaccg cgccaatcac
     2281 gcccgcaccc acactgctcc acgtgtagtg gaacgaggac ccgagaaccg cgcccgcgaa
     2341 gccgcccgcg accagtcgtg cgccgaacgc cggccccgtc tttcggctcg gggtcttcgg
     2401 cagcggatcg gtgagcaatt cgatgacggc cagcacggtg aagacagtca ccgcggcggg
     2461 atggctgacc cagtgaagcc agcggttgtc gagatcaaac cagcccagaa acgccgccca
     2521 actgaccact gcgggcgcgg tgagcgcacg caacccggcc accacgccga tgagtagagc
     2581 aagcacaatc accgcaacgt gcgtcatccc ccggacgcta gcaccgagac cgctcagaag
     2641 cgacagaacc ggatatcaga cgccagaatc gctttggcgc caatgccggc gagttcgtcc
     2701 atgatcgtgt tgacgtcttt gcgcggcacc agcgcccgca ccgccaccca gtcggggtcg
     2761 gccagcggcg caatggtcgg cgactccagc cccggtgtga tcgcggcagc ttggtccagc
     2821 accacgcggg ggcagtcgta gtcgagcatc aggtattgct ggccgaacac cacaccctgc
     2881 acccttgcag ccaactggtc gcgggcggcg gccgtctg
//
LOCUS       DASWBF010000343         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_360281,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000343 DASWBF010000000
VERSION     DASWBF010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360281"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="VF874_12705"
     CDS             complement(<1..1658)
                     /locus_tag="VF874_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9176890.1"
                     /translation="MTAAVVGAAGVAQTDRTEFTFGLRADGYDRRAELTRLELDTLLE
                     LGPVGVRRSRARGAAQRAVLWDSVAVLVEPLDEARAVLHHPARARYRRASVHAAGLVL
                     QHCAESGRGYWGWRTDDWAGLCGSSVEAFLSARVTPTETTVRPFLVAIAYLVARFDGF
                     HLLGTFNRLHLARLVFGDAAVEASTQRVGEILDGWGYRSALSARHSLRGALGQVMLIN
                     RSPRLADLDNDAFTRLRDHPATNQHHGQLLFALQRVVASLGHCDPPVRLGRNHIPAIE
                     GAAAAWIGWVERWCATSTLTPKVRATIRTQMARAGRWLVAEHPEITEPSQWTRQTCAS
                     WVAAVDRMRVGDHVQRRDGLAARVGAPISPRTKAHVLTCTRTFFRDCQEWEWFGRRFD
                     PARALAVPRSVAALIGTDPRVISDDVWAKLLWAGLNIESDDLPANTAGTYYPMPLIRA
                     VTLTWLFSGLRSDELSRLRVGCVRWQHDGAPTTADARDVPVTDAVCLIDVPVHKTGTA
                     FTKPVDPLLGQAIEAWQAARPAQPATIDRNTSEHVDMLFAVRGQP"
     gene            complement(1655..2725)
                     /locus_tag="VF874_12710"
     CDS             complement(1655..2725)
                     /locus_tag="VF874_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9176891.1"
                     /translation="MQMMMVEQKWPVFGRHELAVEWLRVWADLGRAPRTIDAYARALA
                     EYLLSCENAGVDPIAASRAQVAAFVRELTSRPNHLGVNVVSLDSGVGLANATLQQRLV
                     AVRLFYDYLIEEGRRDCNPVGRGRYTPRSRFGGGGDRGLVPALVKLPWIPSERQWLDV
                     LAGFADEPIRNRVMLALAYDAALRREELCSLRTDDLDPAHRMLRVRAETTKTRRERMV
                     PYSAAAGALLAQYLTHRGTLSRARGSLFLSESRRNRAEPLTLWTWSKVVRRIALAANV
                     PQFSTHTTRHLCLTDLARMGWELHAIATFAGHRSTESTMRYIHLSGRELSDKLNRATA
                     VLRAQRIGMLTATATVGAGEQR"
BASE COUNT          430 a          985 c          975 g          399 t
ORIGIN      
        1 ggctggccgc ggacggcgaa gagcatgtcg acgtgttcgc tggtgttgcg gtcgatggtg
       61 gccggctgtg cgggtcgggc ggcttgccag gcctcgatgg cctgcccgag cagcggatcg
      121 acgggtttgg tgaacgcggt gccggtcttg tgcacgggca cgtcgatcag gcagacggcg
      181 tcggtgaccg gcacgtcgcg ggcgtcggcg gtggttggtg cgccgtcgtg ctgccagcgc
      241 acgcagccga cccgcagccg ggacagttcg tcgctgcgca ggccgctgaa cagccaggtg
      301 agcgtcaccg cgcggatcaa cggcatcggg tagtaggtgc cggcggtgtt ggcgggcagg
      361 tcgtcggatt cgatgttcag cccggcccac agcagcttgg cccacacgtc gtcggagatg
      421 accctcgggt cggtgccgat cagcgcggcg acgctgcgcg gcaccgccag cgcgcgggcg
      481 gggtcgaagc ggcggccgaa ccattcccat tcctgacagt cgcggaagaa ggtcctggtg
      541 caggtcagga cgtgggcctt ggtgcgtggg gagatcgggg cgccgacgcg tgcggcgagg
      601 ccgtcgcggc gctgcacgtg gtcgccgacc cgcatccggt ccacggcggc gacccaggac
      661 gcgcaggttt gtcgggtcca ctggctcggt tcggtgatct cggggtgctc ggccaccagc
      721 caccgcccgg ccctggccat ctgggtgcgg atggtggcgc gaaccttggg ggtcagtgtt
      781 gaggtcgcac accaccgttc gacccagccg atccacgccg cggcggcacc ctcgattgcc
      841 ggtatatggt tgcgtcccaa gcgaaccggt ggatcgcagt ggcccagtga ggcgaccacg
      901 cgttgcagcg caaacagcag ctggccgtgg tgttggttgg tggccggatg gtcccgtagc
      961 cgggtgaagg cgtcattgtc gagatcggcg agccgcgggc tgcggttgat cagcatgacc
     1021 tgcccgagcg cgccacgcag agagtgccgg gcgctcagcg cactgcggta gccccacccg
     1081 tcgaggatct cgcccacccg ctgcgtcgac gcctcgaccg cggcatcacc gaacacgagc
     1141 cgggccaggt gcagccggtt aaaggtaccc agcaggtgga agccgtcgaa gcgtgcgacc
     1201 aggtaggcga tcgcgaccag gaacgggcgc accgtggtct cggtcggtgt cacccgtgcg
     1261 gacaggaacg cttccacgct ggagccgcac aggccggccc agtcgtcggt ccgccagccc
     1321 cagtaaccgc gaccggactc ggcgcaatgc tgcagcacga gcccggccgc gtgcacgctg
     1381 gcgcggcggt agcgcgcgcg ggccgggtgg tgcagcaccg cacgtgcctc gtcgagcggc
     1441 tcgaccagca cagccacgct gtcccacaac acagcgcgct gggctgcgcc gcgggcgcgg
     1501 ctgcggcgca cccccaccgg gccgagctcg agcagggtgt ccagttccag ccttgtcagc
     1561 tccgcgcggc ggtcgtagcc gtcggcgcgc aacccgaacg tgaactcagt ccggtccgtc
     1621 tgcgccacgc cggcggcacc aacaacggcg gcggtcaccg ctgctccccg gcgccaaccg
     1681 tggcggtcgc ggtgagcatg ccgatccgtt gggcccgcag gacggccgtg gcgcggttca
     1741 gcttgtccga cagctcccgg ccggacaggt ggatgtagcg catcgtcgac tcggtcgagc
     1801 ggtgtccggc gaaggtggcg atcgcgtgca gctcccaacc catccgggcc aggtcggtca
     1861 aacacaggtg ccgagtggtg tgcgtggaaa actgtggaac gttggcggcc aaggcaattc
     1921 gacgcaccac cttcgaccag gtccacagcg tcagcggctc ggcccggttg cgccgcgact
     1981 cggacaaaaa cagcgacccc cgggcccggc tgagcgtgcc ccgatgcgtc agatactgcg
     2041 ccagaagcgc cccggccgcc gccgagtacg gcaccatccg ctcccggcga gtcttcgtcg
     2101 tctccgcccg cacccgcagc atccggtgcg cgggatccag gtcgtcggtg cgcaggctgc
     2161 acaactcctc ccgccgcaga gcggcgtcgt aggccagcgc gagcatcacc cggttgcgga
     2221 tcggctcgtc ggcgaacccg gcgagcacat ccaaccactg ccgttccgac gggatccacg
     2281 gcagcttcac cagcgccggc accagcccac gatcaccgcc accgccgaag cggctgcgcg
     2341 gggtgtaccg gccacggccg accgggttgc aatcccgccg cccctcctcg atcaggtagt
     2401 cgtagaacag ccgcaccgcg accagccgct gctgcaacgt cgcgttcgcc aaccccactc
     2461 cggagtccag cgaaaccacg ttcacgccaa ggtgattcgg tcggctggtc agctcacgaa
     2521 caaacgcagc aacctgcgcc cgggacgccg cgatcgggtc gacaccggcg ttctcacacg
     2581 agagcaggta ttccgccagc gcccgcgcat acgcgtcgat cgtgcgcggt gcgcgcccca
     2641 gatcggccca cactcgcagc cactcgaccg ccaactcgtg ccgcccgaac accggccact
     2701 tctgctccac catcatcatc tgcatgccgg taggtatccc tcagttcgtg gtgacgatcc
     2761 aggaccgcag gccccgtgtc gtgattcca
//
LOCUS       DASWBF010000344         9603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_814354,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000344 DASWBF010000000
VERSION     DASWBF010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814354"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1719
                     /locus_tag="VF874_12715"
     CDS             82..1719
                     /locus_tag="VF874_12715"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HEX9176892.1"
                     /translation="MSSDAPPSLHAGRLIARRLKASGIDTVFTLSGGHLFSFYDGCRS
                     EGIRLVDTRHEQTAAFAAEGWSKVTRVPGVAALTAGPGVTNGMSAMAAAQQNQSPLVV
                     LGGRAPALRWGQGSLQEIDHVPFVAPLARFAATASSAESTGTLVDEALRAAAAAPSGV
                     AFVDFPMDHVFSEAHDPGLPGALSDVPAASNADATALDRAADLLAGAARPVIMAGTSV
                     WWGRAEPALLQLAERGRIPALMNGMARGVVPADHELAFSRARSRALKEADVALVVGVP
                     MDFRLGFGGVFGADTQLIVADRAEPVRPHPRAVAAGLYGDLSTVLSTLSNARSPDREA
                     WIADLRAVETAARAAENAELSDDRTPLHPMRVYAELAPMLDRDAIVVIDAGDFGSYAG
                     RVIDSYVPGAWLDSGPFGCLGSGPGYALAAKLARPDRQVVLLQGDGAFGFSGMEWDTL
                     VRHDIQVVSVIGNNGIWALEKHPMELLYGYSVVAELRPGTRYDEVVRALGGHGELVSS
                     PAELRPALERAFGAGLPAVVNVLTDPTVAYPRRSHLA"
     gene            1771..4107
                     /gene="secA2"
                     /locus_tag="VF874_12720"
     CDS             1771..4107
                     /gene="secA2"
                     /locus_tag="VF874_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEX9176893.1"
                     /translation="MAKSSTAKPGRLSSTFWKLLGASTEKDQSRSLALVKQSAEFDRK
                     AADLDDEQLRKAAKLLELDDLAESRDIPQFLAIAREAAERNTDLRPFDVQLLGALRML
                     AGDVIEMATGEGKTLSGAIAAAGYALAGRNVHVITINDYLARRDAEWMGPLLEAMGLT
                     VGWVAAESTADERREAYKCDVTYASVNEVGFDVLRDQLVTDVDDLVSPNPDVALIDEA
                     DSVLVDEALVPLVLAGTTHRETPKLEIIRLVGELTAGRDYDTDEDSRNVHLTEAGAQK
                     VEAKLGGIDLYSEEHVGTTLTEVNVALHAHVLLHRDVHYIVRDDTVHLINASRGRIAQ
                     LQRWPDGLQAAVEAKEGIETTETGEVLDTITVQALINRYPTVCGMTGTALAAGEQLRQ
                     FYKLGVSPIPPNTPNIREDEADRVYITAAAKNDAIVEHIAEVHETGQPVLIGTRDVAE
                     SEELHEKLVKAGVPAVVLNAKNDAEEAAIIAEAGKLGAVTVSTQMAGRGTDIRLGGSD
                     VADHSAEKKKVAELGGLHVIGTGRHHTERLDNQLRGRAGRQGDPGSSVFFSSAEDDVV
                     TANLDPGKLPTETDDDGRILSPKAAGLLDHAQRVAEGKLLDVHAMTWRYNELIAQQRA
                     IIVERRDTLLRTPTAREELEERSPKRYEELSEELSEEQLERICRLIMLYHLDRGWADH
                     LAYLADIRESIHLRALGRQNPLDEFHRMAVDAFASLAADAIEAAQQTFETANVLDEEP
                     GLDLSKLARPTSTWTYMVHDNPLADDTLSALSLPGVFR"
     gene            4117..4743
                     /locus_tag="VF874_12725"
     CDS             4117..4743
                     /locus_tag="VF874_12725"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9176894.1"
                     /translation="MEPQGRRHPAPNQPRFKERDRVLTVPNALSVLRLLLVPVSLWLL
                     LVEHADAWATGILMVSGASDWLDGKIARLMGQSSRLGELLDPAVDRIYMVAIPIAFAL
                     RHFVPWWFVIVLVARDALLAATLPALRSRGLNALPVTYIGKGATFGLMSGFPLILLGQ
                     FDGLWSRVVLAIGWGFTIWGFAMYLWSFVLYIAQFVMVVRRMPKVSHD"
     gene            4736..5641
                     /locus_tag="VF874_12730"
     CDS             4736..5641
                     /locus_tag="VF874_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX9176895.1"
                     /translation="MTDRALGGYHPHAGLNAHEAGQPQFNPVPSLLRSLLSEHLDPGY
                     AAAAAERTAGTPPRPRSAAWAWQAAAALLIALVFAAAVAQARSTAPGVKETQQVLANS
                     VRSAEAGTDALAGQRGQLSTEVDDLRRSRLEGDAQGQELLRSLDELGFPGASTKVIGP
                     GLTVTLSDPGASPDLSDVSKERIPGSRQVVLDRDLQLVVNSLWASGAEAIAVGGVRIG
                     PNVTIRQAGGAILVDNQPISSPYAIVAIGPPHGLQESFERSPGFVRMRLLEASYGVGA
                     TVSAGDALTEPAGSAREVKYARPIG"
     gene            5658..5990
                     /locus_tag="VF874_12735"
     CDS             5658..5990
                     /locus_tag="VF874_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HEX9176896.1"
                     /translation="MIGIAALVAGIVLGLVFHPSVPEVVQPYLPIAVVAALDAVFGGL
                     RAYLDRIFDSKVFVVSFVFNVLVAALIVYLGDQLGVGTQLSTAIIVVLGIRIFGNAAA
                     LRRKLFGA"
     gene            5995..6819
                     /locus_tag="VF874_12740"
     CDS             5995..6819
                     /locus_tag="VF874_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX9176897.1"
                     /translation="MSEHDAAHHGRHELPADATPPRIGDVQPRRRLGTLRHPTVFGVL
                     AVVLCVVLGAAIATQVRQTESGDALESARPADLVVLLDSLQQREAALNKEISDLQRTL
                     AALKASGSDDKAAIADAQSRLAALSILVGTAAATGPGVTLTIGDPVSGVSAETMIDVI
                     NELRAAGAEAMEIRGSGGQGAPPASGGPAVRVGVDTWVAGSPGALSVDGQTLSPPYSI
                     VAIGDPPTLAAAVNIPGGAVDSVERVGGTITVQQSDRVDVTALRQPKPRQYAQPVK"
     gene            6865..7260
                     /gene="gcvH"
                     /locus_tag="VF874_12745"
     CDS             6865..7260
                     /gene="gcvH"
                     /locus_tag="VF874_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX9176898.1"
                     /translation="MTETPSDLQYTAKHEWVRRTGSDTVRVGITDFAQSSLGDVVFVQ
                     LPEVGSDVTAGEAFGEVESTKSVSDLYAPVSAKVVAVNGDLEGNPQLVNSEPYGDGWL
                     VELQADAAALDEGIAALLDADGYRNTIRE"
     gene            7402..7869
                     /gene="garA"
                     /locus_tag="VF874_12750"
     CDS             7402..7869
                     /gene="garA"
                     /locus_tag="VF874_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen accumulation regulator GarA"
                     /protein_id="HEX9176899.1"
                     /translation="MTDKDSGDQTSEEVTVETTSVFRADFLNELDAPAQAGADTAVSG
                     VEGLPSGSALLVVKRGPNAGSRFLLDQAVTSAGRHPDSDIFLDDVTVSRRHAEFRLEN
                     NEFQVVDVGSLNGTYVNREPVDSAALANGDEVQIGKFRLVFLTGPRGDDGETG"
     gene            7974..8720
                     /locus_tag="VF874_12755"
     CDS             7974..8720
                     /locus_tag="VF874_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9176900.1"
                     /translation="MSAPDTPALAGMSIGAVLDLLRPEFPDITISKIRFLEAEGLVTP
                     QRSASGYRRFTAYDCARLRYILTAQRDHYMPLKVIKAHLDAQPDGELPESAGFAFAAP
                     RLVTVAGQPHDSAPLTNSVVEPPQVRLSREDLLERAGVDVALLTALTKAGVVIAGRGG
                     LFDEHDVVILQCAKALADFGVEPRHLRAFRSAADRQSDLIAQIAGPVGKGGKTGARDR
                     ADDLAREVAALAITLHTALVKSAVRDVLDR"
     gene            8782..9276
                     /locus_tag="VF874_12760"
     CDS             8782..9276
                     /locus_tag="VF874_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX9176901.1"
                     /translation="MGEVRVVGIRVEQPQNQPVLLLREANGDRYLPIWIGQSEAAAIA
                     LEQQGVEPARPLTHDLIRDVIAALGHSLKEVRIVDLQEGTFYADLIFDRNIKVSARPS
                     DSVAIALRVGVPIYVEEAVLAEAGLLIPDENDDEASGAVREEEVEKFKEFLDSVSPDD
                     FKAT"
BASE COUNT         1585 a         3110 c         3401 g         1507 t
ORIGIN      
        1 atgcgacctc ctgacgaact gtgggcgaag gtacctcagc ggacggcatt cggcccgtgg
       61 ccggtatcta ggctgacggt catgagctcc gacgctccgc cctcgctgca tgccggccgc
      121 ctgatcgcgc gtcgcctgaa ggccagcggc atcgacaccg tgttcacgct gtccggcggc
      181 cacctgttct cgttctacga cggctgccgc agcgaaggca tccggctggt cgacacccgc
      241 cacgagcaga ccgccgcgtt tgcggcggag gggtggtcga aggtgacgcg ggtaccgggc
      301 gtggcggcgc tgaccgccgg gccgggtgtc accaacggca tgagcgcgat ggcggcggcg
      361 cagcagaacc agtcgccgct ggtggttctt ggtggccgcg cgccggccct gcgatgggga
      421 caggggtcgc tgcaggaaat cgaccacgtg ccgttcgtcg cgcccctggc ccggttcgcc
      481 gcgaccgcca gctcggccga gtcgaccggg acgctggtcg acgaggcgtt gcgggccgcg
      541 gcggctgcgc cgtccggtgt ggcgttcgtc gacttcccga tggaccacgt gttctcggag
      601 gcgcacgatc ccgggctgcc gggcgcgctt tccgacgtgc cggccgcctc caatgccgac
      661 gccaccgcgc tggaccgggc cgccgacctg ctggcgggtg cagcgcggcc ggtgatcatg
      721 gccgggacca gtgtgtggtg ggggcgggcc gagccggcgc tgttgcagct cgccgagcgg
      781 ggacggattc cggcgctgat gaacggcatg gcccgcggcg tcgtgcccgc cgaccacgaa
      841 ctggcctttt cccgggcccg gtcccgggcg ttgaaagagg ccgacgtcgc gctggtcgtc
      901 ggcgttccga tggacttccg gctcggattc ggcggcgtgt tcggcgccga cactcagctg
      961 atcgtcgccg accgcgccga acctgtccgt ccgcaccctc gcgccgtcgc agcggggctg
     1021 tacggcgacc tgtcgacggt gctatccacg ctgagcaacg cacggtcacc cgacagagag
     1081 gcgtggatcg ccgaccttcg agccgtcgaa acggcggccc gggctgccga gaatgccgag
     1141 ctgtccgatg accgcacgcc gctgcacccg atgcgggtgt atgccgagct ggccccgatg
     1201 ctggaccgcg acgcgatcgt cgtcatcgac gccggcgact tcgggtcgta cgccggtcgc
     1261 gtgatcgaca gctacgtccc cggcgcgtgg ctcgacagcg ggccgttcgg atgcctgggc
     1321 tccgggcccg gctacgcgct ggccgccaag ctggcgcggc ccgaccgcca ggtcgtgctg
     1381 ctgcagggcg acggcgcgtt cgggttctcg ggaatggagt gggatacgtt ggtgcgccac
     1441 gacattcagg tggtgtcggt gatcggcaac aacggcattt gggcgctgga aaagcatccg
     1501 atggagctct tgtacgggta ttcggtggtg gccgagttgc ggcccggcac ccgctacgac
     1561 gaggtggtgc gcgcgctggg cggccacggc gagctggtgt ccagcccggc ggagctgcgt
     1621 cccgcgttgg agcgggcctt cggtgcgggc ttgcccgccg tcgtcaacgt gctgaccgac
     1681 ccgaccgtcg cctacccacg gcgctcgcac ttagcctgaa ttgccgcctt cggcggcggg
     1741 cgctgactac gagccgcgta ccgttgtgct gtggcgaaat cgagtaccgc taagccgggt
     1801 cgcctgagca gcacattctg gaagctgctc ggcgccagca ccgagaagga tcagagccgt
     1861 tctctggctt tggtcaagca gtcggccgaa ttcgacagga aggccgccga tctcgacgac
     1921 gagcagctgc gcaaggccgc gaagctgctc gaactcgacg acctcgccga atcccgagac
     1981 atcccacagt tcctggcgat cgcgcgagag gcggccgagc gcaacaccga cctccgaccg
     2041 ttcgacgtgc agcttctcgg tgcgctgagg atgctggccg gcgacgtcat cgagatggcg
     2101 accggcgaag gcaagacgct gtccggcgcg atcgccgccg ccgggtatgc gctggcgggc
     2161 cgaaacgtgc acgtcatcac gatcaacgac tacctggccc gccgcgacgc ggaatggatg
     2221 ggtccgctgc ttgaggcgat gggcctgacc gtcggctggg ttgccgccga gtcgacggcc
     2281 gacgaacgcc gcgaagccta caagtgcgac gtcacatacg cgtcggtcaa cgaggtcggc
     2341 ttcgacgtgc tgcgcgacca actcgtcacc gacgtcgacg acctggtgtc gccgaacccg
     2401 gacgtcgcgc tgatcgacga ggccgattcc gtgctcgtcg acgaggcgct ggtgccgctg
     2461 gtgctggccg gcaccaccca ccgcgagacg ccgaagctgg agatcatccg gctggtcggc
     2521 gagttgaccg caggcaggga ctacgacacc gacgaggaca gccgcaacgt gcacctcacc
     2581 gaggccggcg cgcagaaggt agaggcgaaa ctcggtggca tcgacctgta ttcggaagag
     2641 cacgtcggca ccacgctcac cgaggtgaac gtcgcgctgc acgcgcacgt gctgctgcac
     2701 cgcgacgtgc actacatcgt gcgcgacgat acggtgcatc tgatcaacgc gtcgcgcgga
     2761 cggatcgctc agttgcagcg ctggccggac ggcctgcagg ccgcggtcga ggccaaggag
     2821 ggcatcgaga ccaccgagac cggcgaggtg ctggacacga tcacggtgca ggcgctgatc
     2881 aaccggtatc cgacggtatg cgggatgacg ggcacggcgc tggccgccgg cgagcagctg
     2941 cggcagttct acaagctggg tgtgtcgccg atcccgccga acaccccgaa catccgcgaa
     3001 gacgaggccg accgggtgta catcaccgcc gcggccaaga acgacgcgat cgtcgagcac
     3061 atcgccgagg tccatgaaac aggccagccg gtgttgatcg gcacccgcga tgtcgcggaa
     3121 tccgaagagc tgcacgagaa attggtgaag gccggcgtcc cggccgtcgt cctcaacgcg
     3181 aagaatgacg ccgaggaggc ggcgatcatc gccgaagccg gcaagctcgg cgcggtcacg
     3241 gtctcgacgc agatggccgg ccggggcacc gacatccgac tgggcggatc cgacgtagcc
     3301 gaccactccg ccgagaagaa aaaagtcgcc gagttgggcg gcttgcacgt gatcggcacg
     3361 ggtcggcatc acaccgagcg cctggacaac cagttgcgcg ggcgtgccgg acgccagggc
     3421 gatccgggat cgtcggtgtt cttctcgagt gccgaagacg acgtcgtgac cgccaacctc
     3481 gatccgggca agctgcccac cgaaacggac gacgacggtc gcatcctcag cccgaaagcg
     3541 gcgggactgc tggatcacgc ccagcgggtg gccgagggca aactgcttga cgtgcacgcc
     3601 atgacgtggc ggtacaacga gctgatcgcc cagcagcggg cgatcatcgt ggaacgccgt
     3661 gacacgctgt tgcgcactcc cactgcgcgg gaagagctgg aggagcggtc gccgaagcgc
     3721 tacgaggagc tttctgaaga gctgagcgag gagcagctgg agcggatctg ccggttgatc
     3781 atgctgtacc acctggaccg cggctgggca gatcacctcg cgtacctggc cgacatccgg
     3841 gagagcatcc acctgcgcgc gctgggccgg cagaatccgc tcgacgagtt ccatcggatg
     3901 gccgtcgacg cgttcgcatc gctggcggcc gacgcgatcg aggcggcgca gcagacgttc
     3961 gagacggcca acgtcttgga cgaggagccg ggactcgact tgtcgaagtt ggcgcggccg
     4021 acgtcgacgt ggacgtacat ggtgcacgac aacccgttgg ccgacgacac tctgtcggcg
     4081 ctgagtctgc ctggggtttt ccgctaaggt tcggccatgg agccgcaggg ccggcgacat
     4141 ccggcgccga accagccgcg cttcaaggag cgcgaccgag tgctgaccgt gcccaacgcg
     4201 ctcagtgtgc tgcggctgct gctcgttccg gtgtcccttt ggctgctgct cgtcgagcac
     4261 gccgacgcgt gggccaccgg gattctgatg gtctccggcg catcggactg gctcgacggc
     4321 aagatcgccc ggctgatggg ccagtcgtca cggctcggcg aattgctcga tccggcagtc
     4381 gaccgcatct acatggtggc gatcccaatt gcgttcgcgc tcaggcactt tgtgccatgg
     4441 tggttcgtca tcgtgctggt cgcccgcgat gcgctgctgg cggcgacgct gccggcgctg
     4501 cgaagccgcg gcctgaacgc gctgccggtc acctacatcg gcaagggcgc gacgttcggg
     4561 ctgatgtcgg gtttcccgct tattctgctg ggtcagttcg acgggttgtg gagccgcgtc
     4621 gtgctcgcga tcgggtgggg cttcacgatc tggggcttcg cgatgtacct gtggagcttt
     4681 gtgctctaca tcgcgcagtt cgtcatggtc gtgcggcgga tgccgaaggt gtctcatgac
     4741 tgaccgggca cttggcgggt accacccgca cgctggcctg aatgctcatg aagccggcca
     4801 gccgcagttc aatcccgtgc cctcgctgct gcgatctctg ctgtccgaac acctcgaccc
     4861 gggctatgcc gcggcggccg ctgagcgcac cgccggcacg ccgccccgac cgcgctcggc
     4921 agcgtgggcg tggcaggctg cggcggcgct gctgattgcg ctggtgttcg cagcggccgt
     4981 cgcgcaggcg cggtcgacgg cacccggcgt caaggaaacg cagcaagtgc tggccaacag
     5041 cgtccggtcc gcggaagccg gcacggacgc gttggccggg cagcgtggcc agctttccac
     5101 cgaggtggac gacctgcgtc gcagccggct cgagggcgat gcgcagggcc aggagttgct
     5161 gcgcagtctc gacgagctcg gttttccggg tgcgtccacc aaggtgatcg gaccgggact
     5221 gacggtgacg ctcagcgacc ccggggcctc gcccgatctg tccgacgtgt ccaaggagcg
     5281 aatccccggc agccgacagg tggtgctcga ccgcgatctg cagctggtgg tgaactcgct
     5341 gtgggcaagc ggcgccgagg ccatcgcggt cggcggcgtg cgaatcggcc ccaacgtcac
     5401 gatccggcag gcgggcggcg cgattctggt tgacaaccag ccgatcagta gcccgtacgc
     5461 gatcgtggcg atcggaccac cacacggact gcaggaaagc ttcgagcgca gccccggctt
     5521 tgtgcggatg cggctgctgg aggcgtcgta tggcgtggga gccaccgtca gtgccggcga
     5581 cgcgctgacc gagccggcgg gcagcgcgcg agaagtcaag tacgccaggc cgattggatg
     5641 aacaagggga gacgacattg atcggtatcg ccgcacttgt cgcgggcatc gtgctgggcc
     5701 tggtgtttca tcccagcgtg ccggaggtgg tgcagccgta tctgccgatt gccgtggtgg
     5761 ccgcactgga cgcggtattc ggcggcctgc gggcatacct cgaccgcatc ttcgactcca
     5821 aggtgttcgt cgtttccttc gtgttcaacg tgctggtcgc cgcgctgatc gtctacctcg
     5881 gcgaccagct aggtgtcggt acgcaattgt cgacggcgat catcgtggtg ctgggcatcc
     5941 ggatcttcgg caacgccgcc gcgctgcggc gcaagctgtt cggggcatga ccgcatgagc
     6001 gaacacgacg ccgcgcacca cggtcgtcac gagctgccgg ccgacgcgac gccacctcgg
     6061 atcggcgacg tccagccgcg ccgacggctc ggcaccctgc ggcacccaac cgtgttcgga
     6121 gtgctggccg tggtgctgtg cgtggtgctc ggcgcggcga tcgcgacgca ggtccggcag
     6181 accgagtcgg gcgacgcgct ggaatccgcc cggccggcgg acctggtggt gctgctggat
     6241 tcccttcagc agcgggaggc ggcgctgaac aaggagatat cggacttaca gcgcacgctg
     6301 gctgcgttga aggcgtcggg gagcgacgac aaggcagcca tcgccgatgc gcagtcacgg
     6361 ctcgcggcac tgtcgatcct ggtcggcacc gcggcagcga ccggaccggg agtgacgttg
     6421 acgatcggcg atccggtgag cggtgtgtcg gccgaaacga tgatcgatgt catcaacgag
     6481 ttgcgggcgg ccggggcaga agcgatggag atccgtggct cgggcggaca gggggcaccg
     6541 cccgccagcg ggggacccgc ggtgcgcgtg ggcgtcgaca cctgggtggc cggcagtccc
     6601 ggcgcgctgt cggtcgacgg tcagactctg agcccgccgt attcgattgt ggcgattggc
     6661 gatccgccaa cattggcggc cgcggtcaac atccccggag gagccgtcga cagcgtcgaa
     6721 cgggtcgggg gcacgatcac cgtgcagcag tccgaccgcg tcgacgtcac cgccttgcgg
     6781 caaccgaaac cgcgccaata cgctcagccc gtcaaatgac cgcatgacca cggccccaca
     6841 ggccgcgaca ggtaaggagc cccggtgacc gaaaccccgt ccgacctgca gtacaccgcc
     6901 aaacacgagt gggtgcgccg cacgggctcc gacaccgtcc gggtgggaat caccgacttc
     6961 gcgcagtcct cactgggcga cgtggtgttc gtccaattgc ccgaggtcgg ctcagacgtg
     7021 actgcaggcg aagcctttgg tgaagtggaa tcgaccaagt ccgtgtccga cctgtatgcg
     7081 ccggtgtcag cgaaagtcgt tgcggtcaac ggcgatttag agggcaaccc gcaactggtc
     7141 aactcagaac cgtatggcga cggttggctg gtggaactgc aggccgatgc agctgcactc
     7201 gatgagggca tcgccgcgct gctggatgct gacggctacc gcaacacgat cagagagtga
     7261 cgcgattgct agggttcacc gacggatttt gcctcgcggc cgtggccgcg gggtacggtt
     7321 tgaggagcag ggcgcaggtc ggctcatgac gtcgcaggac accgcggccg accccgccag
     7381 agcagccagg aggagcagcg ggtgaccgac aaggactcag gggaccagac gtctgaagaa
     7441 gtcaccgtgg aaacgacctc cgtttttcgc gcggacttcc tcaacgaact ggacgcgccg
     7501 gcccaggcgg gtgcggacac cgcagtgtcg ggcgtcgaag gcttgccgag cgggtcggcg
     7561 ttgctcgttg tcaagcgcgg gcccaacgcg ggctcgcggt tcctgctcga tcaggccgtg
     7621 acgtcggcgg gccgacatcc tgacagcgac atcttccttg acgacgtcac cgtcagccgc
     7681 cggcatgccg agttcaggct ggagaacaac gaattccagg ttgtcgacgt cggcagcctc
     7741 aacggcacgt acgtcaaccg cgagccggtg gactctgccg cgctggccaa cggcgacgag
     7801 gtgcagatcg gcaagttccg cctggtgttc ttgaccggac cccggggcga cgacggcgag
     7861 accgggtaga gccaggcggc gatgagcgct tgcgcgaaga gcagataacc gatgagcgct
     7921 tgcgcagcga tgagcccctt gggcgaagaa cctcagaatc agagcagata acagtgagcg
     7981 cgcccgatac gcccgcgctg gccggcatgt ctatcggagc ggtgctcgac ctcctccggc
     8041 cggaattccc tgacatcacg atctccaaga tccggtttct ggaggccgag ggcttggtca
     8101 cgccgcagcg cagcgcatcg ggataccgcc ggttcacggc ctacgactgt gcgcggctgc
     8161 gctacatcct caccgcccag cgcgaccact acatgccgct gaaggtgatc aaggcccatc
     8221 tggacgcgca accggacggc gaactgccgg agtcggccgg attcgccttc gccgcaccgc
     8281 gattggtgac cgtggcaggc cagccccatg actcggcacc actgaccaac agcgttgtgg
     8341 agccgccgca ggttcgcctg agccgcgaag acctgctcga gcgtgccggc gtcgacgtgg
     8401 cgttgctgac cgcgctgacg aaagcgggtg tggtgatcgc gggccgcggg gggctgttcg
     8461 acgagcacga cgtggtgatc ctgcagtgcg ccaaggcgtt ggccgacttc ggtgtcgagc
     8521 cacggcatct gcgggcgttc cggtctgcgg ccgaccggca gtccgatctg atcgcacaga
     8581 tcgcgggccc cgtgggcaag ggaggtaaga ccggtgcccg cgaccgggcc gacgacttgg
     8641 cgcgcgaagt tgctgcgctg gcgatcacgt tgcacacggc gttggtcaag tcggctgtgc
     8701 gcgacgttct cgaccgctga ggattagact ggcgacggag tcttgttggc tggcttcgtc
     8761 aggtccggag ggcaggcaca tatgggtgag gttcgtgtcg tcggcatacg cgttgagcag
     8821 ccgcagaacc agccggtgct gctgctgcgc gaggccaacg gcgatcgcta tctgcccatt
     8881 tggatcggcc agtccgaggc tgcggcgatc gcgctcgagc agcagggcgt cgagccggcg
     8941 cggccgctga cccacgacct gatccgagat gtcattgccg cgcttgggca ttcgctcaag
     9001 gaagtgcgga tcgtggatct gcaggaaggc acgttctacg ccgacctgat cttcgaccgc
     9061 aacatcaaag tgtccgcgcg gccgtcggac tccgtggcca tcgcgctgcg ggtaggcgta
     9121 ccgatctacg tggaggaggc ggtgctcgcc gaggctggcc tgctgattcc cgacgaaaac
     9181 gacgacgagg cttcgggcgc tgtccgcgag gaggaagtcg agaagttcaa agagttcctc
     9241 gatagcgtct cacccgacga tttcaaggcg acctgaacgg gcagcaccca catcacggat
     9301 gcgtctcaac ttgccagagc cgctccgaca cgcgggggag ggtcacgttg accggtcctc
     9361 gcagcggcca taatttggtc gtaacggcag gcgcagcggc ccgcccggcg cgtatgctcg
     9421 agcaactcga gccgttacgc gaacgatggc cagtcgatag ccaacgcgac aggcgggagg
     9481 tagcacggtg gatgatcatc cacaccagga gcagctggac ctgaacgcga gcgacaccac
     9541 caccacgcgc cgcgaggcgg cgccgaacga gccggtgcag cccggcctgt tccccgacga
     9601 ctc
//
LOCUS       DASWBF010000345         5359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_881949,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000345 DASWBF010000000
VERSION     DASWBF010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881949"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..500)
                     /locus_tag="VF874_12765"
                     /pseudo
     CDS             complement(1..500)
                     /locus_tag="VF874_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            596..1894
                     /locus_tag="VF874_12770"
     CDS             596..1894
                     /locus_tag="VF874_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9176902.1"
                     /translation="MAAVNRVYAARLAGMVVLGPDGESIGRVRDVVISISIVRQQPRV
                     IGLVVELLTRRRIFVPITRVTAIEPNAVTLTTANVSLRGFAQRPGEVLVLGQVIETRV
                     RVDDPELEQLAGMDVVVVDLGVEQTRTREWVVTRVAVRSLRRLGRRSNIYIVDWQNVQ
                     GLTPSALALPGQGVAQLLHQFEEQRPIEVAEAIRELPTKRRVEVINALDDERLADVLQ
                     ELPEDEQSALLMQLDTERAADVLEAMEPDDATDLLQVINPADRELLLRRMDPEDSEPV
                     RRLLSHSPDTAGGLMTSEPVVLAPDTTVAEALARVRDPDLTPALASLVFVVRPPTATP
                     TGRYLGCVHLQRLLRDPPAALVSGMVDTDLPSLTPESSLAAVTRYFAAYNLVCGPVVD
                     EQNHLLGAVTVDDVLDHLLPDDWRVHFEEAELSSSAEGVT"
     gene            1891..2427
                     /locus_tag="VF874_12775"
     CDS             1891..2427
                     /locus_tag="VF874_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX9176903.1"
                     /translation="MSESRQRLDTPRTSRRLSFNVDPEAVGQFSESIARFLGTGRYLL
                     WQTILVIVWIALNLFAVTLRWDPYPFILLNLAFSTQAAYAAPLILLAQNRQENRDRVS
                     LEEDRRRAAQTKADTEYLARELAALRLAIGEVATRDYLRRELDELREIIEGSLPASSQ
                     SDAPPKGDLADRRAKRVT"
     gene            2669..3958
                     /locus_tag="VF874_12780"
     CDS             2669..3958
                     /locus_tag="VF874_12780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX9176904.1"
                     /translation="MVAPVVFAGAAAGAPRPAPVVHPPTQDANVMPLAAVAPNPGDIS
                     GPSVIQVSRPPSSFHVAAASLSAPPPAVVVNSPGALRIPSMALSAYRNAERMMSLAQP
                     GCGVSWNLLAGIGRIESLHANNGATDARGTAVRPIYGPTLDGSLPGNEVILQSMAPNG
                     RPTYARAMGPMQFLPGTWARYASDGDGDGKADPQNLFDSALAASRYLCSGGLNLRDQS
                     QVLSAILRYNNSMPYARNVLGWAAAYTTGVIPVDLPAITGPIPPMGDAHLETGPEGLG
                     PGLPINVLGLPMTDPMTQMQLVNFGSTDVANQMPGFFPPGMDPNQSAAASPGCPTLVC
                     FGASAMPSVTPPSATPFGPPLPGMGPTNQWPGVMPGPAQAAPLPGPMPALPAPAPPAM
                     PQPMLPWLPPPPAPVEPTPAPAPVLPPPSGPLPGPAA"
     gene            4038..5180
                     /locus_tag="VF874_12785"
     CDS             4038..5180
                     /locus_tag="VF874_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX9176905.1"
                     /translation="MPENQNDLVTAVRSALAKVIDPELRRPITELGMVKNVTIEPDHG
                     VHVEIFLTTSACPKKTEITDRVTRAVADVPGSGAVKVSLDVMSDEQRTELRKMLRGDE
                     REPVIPFAQPGSLTRVYAVASGKGGVGKSSVTVNLAAAMAARGLSVGLLDADIYGHSI
                     PRMMGTTDRPTQVESMILPPIAHDVKVISIAQFTQGNTPVVWRGPMLHRALQQFLADV
                     YWGDLDVLLLDLPPGTGDVAISVAQLIPTAEILVVTTPQMAAAEVAERAGAIALQTRQ
                     RIVGVVENMSGLLMPDGSTMQLFGEGGGRQVAERLSRAIGADIPLLGQIPLDPAQVTA
                     GDSGVPLVLSAPDSPAGKELRKVADTLAARRRGLAGMPLGIDTARR"
     gene            complement(5201..>5359)
                     /locus_tag="VF874_12790"
     CDS             complement(5201..>5359)
                     /locus_tag="VF874_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase TatB"
                     /protein_id="HEX9176906.1"
                     /translation="MSPRAALTKHLLDGDDSILTGKFDKPVDLPPDTPVTKPETASGV
                     TPFDADAT"
BASE COUNT          809 a         1898 c         1810 g          842 t
ORIGIN      
        1 ctagctcggc ggctggaacc gctcgccctg ccgcgtcata cccgcggctc tgcctttggc
       61 cgcgatcacc agcgccatct tgcggctggc ctcgtcgagc atctcatcgc cgagcatcgc
      121 cgcgccgcgc acaccgccgg ccgccgacgt gtgccactcg taggcctcca ggatcagctc
      181 ggcgtggtcg tactcgtctt ggcgcgggct gaaaatcttg ttgccggcgg cgatctgatc
      241 cgggtgcaac acccacttgc cgtcatagcc gagcgccgcc gagcggccgg ccacccgtcg
      301 gaacgcatcg acatcgcgca ccttcagata cggcccgtcg atcgccgcga cgccgtacgc
      361 gcgcgctgcg atcagaatcg acatcagcac atgctccgac agcgcaatcg cggcctgcga
      421 gcgggcctgc gccttggcgt cgacggcgac ggcgttcttc gaggtcgagg aacacctcgt
      481 cggcgggcag actcgatcat cttggcgctg ctggccggca ccgaaagaca cgttcggcgc
      541 ggccgatacg cggtctcggg ccgttgatcc acgcctaact cttacccttg gagtcatggc
      601 ggcggtgaac agggtgtacg cggcacgact ggcgggcatg gtcgtgctgg gccccgacgg
      661 cgaatcgatc ggccgcgtgc gcgacgtggt gatcagcatc agcatcgtcc gccaacagcc
      721 gcgagttatc ggtcttgtcg tcgaattgct cacccgccga aggattttcg tgccgatcac
      781 ccgggtgacc gcgatcgagc ccaacgcggt cacgttgacg accgccaatg tgtcgctccg
      841 cggcttcgcg cagcgacccg gcgaggtgct ggtgttgggg caggtgatcg agacccgggt
      901 gcgtgtcgac gatcccgaac tcgagcaact ggccggaatg gacgtcgtgg tcgtggatct
      961 gggcgtcgag cagacccgga cccgcgagtg ggtggtgacc agggtggcgg tccgaagcct
     1021 gcgccggctg ggccgccgca gcaacatcta catcgtcgac tggcagaacg tgcaaggcct
     1081 gaccccgtcg gcgctggcgt tgcccgggca aggcgtcgcc cagctgctgc accagttcga
     1141 ggaacagcgc ccgatcgagg tggccgaggc gatccgcgag ctgcccacca agcggcgcgt
     1201 cgaggtgatc aacgcgctcg acgacgaacg cctggccgac gtgctgcagg aactgcccga
     1261 ggacgagcag agtgcgctgt tgatgcagct ggacaccgag cgcgccgccg acgtgctcga
     1321 ggcgatggag cccgacgacg ccaccgacct gctccaggtg atcaacccgg cggaccgcga
     1381 gttgctgctg cgccggatgg acccagagga ttccgagccg gtgcgccggc tgctgagcca
     1441 ctctccggac accgcaggcg ggttgatgac gtcggagccc gtggtcctgg cgcccgacac
     1501 caccgtcgcc gaggcgctgg cccgggtgcg cgacccggat ctgacgcccg ccctcgcgtc
     1561 gctggtgttc gtcgtgcgcc cgccgaccgc cacccccacc ggccgctacc tgggctgcgt
     1621 gcacctgcag cggctgctgc gtgacccgcc ggcggcgctg gtcagcggca tggtcgacac
     1681 cgacctgccc agtctgacgc cggaatcgtc cctggccgcc gtgacccgat acttcgctgc
     1741 ctacaatctg gtctgcggcc cggtggtgga cgagcagaat catctccttg gcgcggtcac
     1801 cgtcgacgac gtgctcgacc acctgctgcc cgatgactgg cgcgtgcact tcgaagaagc
     1861 cgaactttcg tccagtgctg aaggggtgac atgagcgagt cgcgtcagcg gctggacacg
     1921 cctcgcacct cacgccgcct gtcgttcaac gtcgaccccg aggcggtcgg gcagttcagc
     1981 gagtcgatcg cgcggttcct gggcaccggg cgctacctgc tgtggcagac catcctcgtc
     2041 atcgtctgga tcgcactgaa cctgttcgcg gtcacgctgc gctgggaccc gtacccgttc
     2101 atcctgctca acctcgcgtt ctcgacgcag gccgcctacg cggcgccgct gatcctcctc
     2161 gcgcagaacc ggcaggagaa ccgcgaccgg gtgagcctgg aagaagaccg gcggcgcgcc
     2221 gcccagacca aagccgacac cgagtacctg gcccgggagc tggccgcgct gcggctcgcg
     2281 atcggcgaag tggcgacccg cgactacttg cgccgcgagc tcgacgaact gagagagatc
     2341 atcgaagggt cgctgccggc gtcgtcgcag agcgacgccc cacccaaggg cgaccttgct
     2401 gaccggcggg ccaagcgcgt cacctagacg tcaagtacct ggtgccaaag atttcacagt
     2461 gaccaatgtg catccattcc gtttgcgaat aggtatggtg attcagttca caaggctagt
     2521 gagtgccggc tcgctcgcgg cgcgggcttc cgacgacgtc aggacggtgt gagtgcacat
     2581 tgggggaggc tcggcccgcg gcgcccttga gtgggcgcgt caacgggttt cccgggtcgc
     2641 tcatacgccc gtgacggccc tcgttctcgt ggtcgccccg gtggtattcg cgggcgcggc
     2701 ggcaggcgcg cctcgcccgg cgccggtggt tcacccgccc acccaagacg ccaacgtgat
     2761 gccgctcgcc gcggtggcac ccaaccctgg cgacatctcc ggtccctccg tcattcaagt
     2821 cagccgcccg ccgtcgagct ttcatgtggc cgcggcgtcg ctctcggcgc ccccgcccgc
     2881 ggtggtggtc aattcgcctg gggcgctgcg cattccatcg atggcgctgt cggcctaccg
     2941 caacgccgag cggatgatgt cgctggccca gccgggatgt ggcgtcagct ggaacctgct
     3001 ggccggtatc ggccgcatcg agtcgttgca cgccaacaac ggcgccaccg acgcccgcgg
     3061 taccgccgtg cggcccatct acgggcccac gctcgacggg tcgctgccgg gcaacgaggt
     3121 catcctccag agcatggcac ccaacggccg gccgacgtac gcccgcgcaa tgggcccgat
     3181 gcagttcctg cctggcacgt gggcccgcta cgcctccgac ggcgacggcg acggcaaggc
     3241 cgacccgcag aacctgttcg actcggcact ggcggccagc cgctacctgt gcagcggcgg
     3301 gctgaacctg cgtgaccaat cgcaggtgct cagcgcgatc ctgcggtaca acaactcgat
     3361 gccctacgcg cgcaacgtgc tcggctgggc ggcggcctac accaccggcg tcatcccggt
     3421 ggatctgccg gccatcaccg ggccgatccc gcccatgggc gatgcgcacc tggaaactgg
     3481 ccccgaaggc ctcggccccg gtctgccgat caatgtgctc gggctgccga tgaccgaccc
     3541 gatgacgcag atgcaactcg tcaatttcgg gtccaccgat gtcgccaacc agatgccggg
     3601 gttcttcccg ccgggcatgg atccgaatca gtctgcggct gcgtcgccgg gctgcccgac
     3661 cctcgtctgc ttcggggcca gcgccatgcc gtcggtgacg ccgccgagcg ctacgccgtt
     3721 cgggcctccg ctgcccggga tgggcccgac caaccagtgg ccgggggtta tgcccggtcc
     3781 tgcccaagcc gcgccgctcc cgggtccgat gccggcgttg ccggctcccg cgccgccggc
     3841 aatgccgcag cccatgctgc cctggctccc accgccgccg gcgcccgtcg aacccacgcc
     3901 ggcacccgcg cctgtgctcc caccgccgag cgggccgctg cccggccccg ccgcctgagc
     3961 agcggcccga tcctgcggtg atgagccgct tgggcgaaga acattaggca cggggccgga
     4021 tcgtacactc gacggtgatg cccgaaaacc agaacgacct tgtgacagcc gtgcgttcag
     4081 ctctggccaa ggtgatcgac cccgagttgc gccggccgat caccgaattg ggcatggtca
     4141 agaacgtcac gatcgagccc gaccacggcg tgcacgtcga gatcttcttg acgacgtcgg
     4201 cgtgcccgaa gaagaccgag atcaccgacc gggtcacccg cgccgtcgcc gacgtgccgg
     4261 gcagcggcgc cgtcaaagtc agcctcgacg tgatgagtga cgaacagcgc accgagctgc
     4321 gcaaaatgct gcgcggcgac gaacgcgaac cggtgatccc gttcgcgcag ccgggttccc
     4381 taacccgggt ctacgcggtc gcgtcgggca agggcggggt tggcaagtcg agcgtcacgg
     4441 tgaacctggc cgccgcgatg gccgcacgcg ggctgtccgt cgggctgctc gacgccgaca
     4501 tctacggcca ctcgatcccc aggatgatgg gcacgacgga tcggcccact caggtcgagt
     4561 cgatgatcct gccgccgatc gcccatgacg tgaaggtgat ctcgatcgcg cagttcaccc
     4621 agggcaacac ccccgtggtg tggcgcggcc cgatgctgca ccgcgcgctg cagcagtttt
     4681 tggccgacgt gtactggggc gatctggacg tcttgctgct ggacctgccg cccggcactg
     4741 gcgacgtcgc gatctcggtg gctcagctga ttcccaccgc ggagatcttg gtggtgacga
     4801 cgccgcagat ggcggccgcc gaggtcgccg aacgcgccgg tgccattgcg ctgcagaccc
     4861 ggcagcggat cgtcggggtg gtggagaaca tgtccgggct gctgatgccc gacgggtcga
     4921 cgatgcagct gttcggcgag ggcggcggac gccaggtcgc cgagcgactg tcccgcgcca
     4981 tcggcgccga cataccgctg ctgggccaga tcccgctgga ccccgcgcag gtgaccgccg
     5041 gcgattccgg ggttccgctg gtcctgtccg caccggattc tccggcgggc aaggaactgc
     5101 gaaaggtcgc cgacacgttg gcggcgcgcc gtcgcgggtt ggccgggatg ccgctgggca
     5161 tcgacaccgc gcgccgctga accgtctccg cagcgtcgcc ctacgtcgcg tcggcgtcga
     5221 atggggttac gccgcttgct gtctcgggct tagtcacggg cgtgtccggg ggcaggtcga
     5281 cgggcttgtc gaacttgccc gtgaggatcg agtcgtcgcc gtccagcaga tgcttggtca
     5341 atgccgcgcg cggcgacat
//
LOCUS       DASWBF010000346         4679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1147474,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000346 DASWBF010000000
VERSION     DASWBF010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147474"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..3582)
                     /locus_tag="VF874_12795"
     CDS             complement(280..3582)
                     /locus_tag="VF874_12795"
                     /inference="COORDINATES: protein motif:HMM:NF020701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HEX9176907.1"
                     /translation="MRARSWFAVLAVGAVVAGSLAVAVPAAVAAPGPATGGPGALSHF
                     DLARKDCLGTARNTTSKIWYTVADGVLSDVYAPTIDTTNVETMQYLVTDGSSFTDLQT
                     RDTTYTVSADSTGMVCTVTSTAKTGAYRLTTTYLTDPARPSVVVQTSYTPLTSAAASY
                     RVYVRLDATVGGNGGGGSGNGGADNAVIDTTTGSPVPVSYDTNTKTNAANRDYAVPSF
                     LALRADQPFGVVSSGFVGTPSDGLAQLDSAHILTPTDSALGGNVEQTAQVRFDKHGDA
                     MLALGFGTTQAHAVATASATVGRPFGSLRGQYRSGWLSYDDALNTPSGSLPGLTPAQQ
                     AAAVNEYYVSANVVKASEDKTFPGAIVASIASPWGQAVSAGDPANTYFGSYREVFSRD
                     LYEAWTGLLTDGDLATARAATRFLLEKQQLPDGSLPRNSLVNGKLAPDSFNTQLDEVA
                     YPILMVLQSGLAGDAGLWPHVQAAANYLIAHGPSFGPERWEEQSGYSPSTIAAEIAGL
                     VAAATIARQHASPADARIWLATADQYQRSIKGWAVTTNGPLSSQPYFIRLSKTGDPNA
                     AISYNVGNGGPTLDQRAVIDAGFLELVRLGELSATDPDVLNSLTVVDKTIEKQSASGS
                     GWLRYNGDGYGDCHILPDHTCPLEGRPWAPSNMGTGHPWPVLSAERGEHDLATGQATS
                     AGQLLAAMGAMSSGVGLVPEQVWDAPNVAASPFGTDPTIASIGFVNGKPDGSAAPLTW
                     GAGSQVRLTADLTAGRLLEQPAQTVDRYITHTQLATTLTVTSPPDQTAATNPIHVAGT
                     AVPGATIDVADVGTGTTTHASGTAAADGSFSFDIAPLIGNNVLVVTSTAPNGGTAQAV
                     RTVVNDVVQGTLLFDATDPTGDDNGPGNYAYPTSSDFHAGAFDLTDFQVYDTGTTVTF
                     RVQTRDLTPTFGSPLGAQLVDVYVHEPGASPTSTAASFPQRNYAIAPGSAWSRLLEVQ
                     GFGQRFVDSTNTNTVGTITISANQISRYITFSVDKAALGGTPASGWIFTVVLTGQDGF
                     SPDQARGFQSTPQPFQLGVCATASSDPHCTVDPATVPKAVDVLTPSSVTQSDELDYTK
                     HQPVVISGVAIP"
     gene            complement(3782..3925)
                     /locus_tag="VF874_12800"
     CDS             complement(3782..3925)
                     /locus_tag="VF874_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176908.1"
                     /translation="MSRGRGSVTIDEELRQIKLGDLGEYLGLVPVRTPLPGRATPPTD
                     AEV"
BASE COUNT          755 a         1489 c         1631 g          804 t
ORIGIN      
        1 atcccagctc ttcgaacacc aagccggcaa aaacgccgtc accacccggg ctttttcagc
       61 gcgctcctag ctattagcta ggaccgactt ggcggcgggc gcctgaccga gcagccgctc
      121 cccgctgctt gcgagggcgg ctgcttcctg ccgggtgtgt acgcccgcca acgaaacagc
      181 ggatacaccg atggcgggct gcatcctttg gcgcgagcgg tagcggaagc caccgaaagc
      241 aacggaatgc tgagggccgc cgtggcggcc cccaaagtgt tacgggatgg ccaccccact
      301 gatgaccacc ggctggtgtt tcgtgtagtc cagctcgtcg gactgggtga cgctagacgg
      361 ggtgagtacg tccacggcct tcggcaccgt ggccggatcg acggtgcaat gtgggtcgga
      421 gctggccgtg gcacacacgc cgagctggaa gggctgcgga gtggactgga aaccgcgtgc
      481 ctgatccggg ctaaacccgt cttggccggt cagcaccacc gtgaagatcc agccagacgc
      541 cggtgtgccg ccgagtgctg ccttgtccac ggagaacgtg atgtaccgcg agatctggtt
      601 ggcgctgatg gtgatggtgc ccaccgtgtt cgtattggtt gaatcgacga agcgctggcc
      661 gaagccctgt acttcgagta gccgactcca tgcgcttccc ggcgcgatcg cgtaattgcg
      721 ttgcgggaag gacgccgctg tcgacgtcgg cgacgcaccc ggctcgtgca cgtagacgtc
      781 gacgagttgc gcgccgagcg ggctgccgaa ggtcggcgtc aggtcgcggg tctggactcg
      841 gaaggtcacg gtagtgccgg tgtcgtagac ctggaaatcg gtcaggtcga acgcgccggc
      901 gtggaagtcc gaggacgtcg gataggcgta attgccgggt ccgttgtcgt cgccggtggg
      961 gtccgtggcg tcaaagagca gcgtgccttg gacgacgtcg ttgacgaccg tccgaacggc
     1021 ctgggccgta ccgccgttgg gcgccgtgct ggtgaccaca aggacgttgt tgccgatcag
     1081 cggcgcgatg tcgaagctga atgacccatc ggcagcagcg gtgccactgg cgtgcgtggt
     1141 cgtccccgtg cctacgtccg ctacatcgat ggtcgcgccg ggcacggccg ttcccgcgac
     1201 atgaatcggg ttggtggccg ccgtctggtc aggcggcgag gtcacggtga gcgtggtcgc
     1261 cagctgggtg tgggtgatat agcggtcgac ggtctgggca ggctgctcga gcaggcgccc
     1321 cgcggtgaga tcggccgtca gccggacctg cgagccggca ccccaggtca aaggtgcagc
     1381 ggagccgtcg ggcttgccgt tgacgaagcc gatcgacgca atcgtcgggt cagtgccgaa
     1441 gggcgacgcg gcaacgttag gcgcgtccca cacctgctcg ggtaccaagc cgaccccgga
     1501 ggacatagcg cccatcgccg cgagcagctg gcctgccgat gtcgcctgcc cggtggccag
     1561 gtcatgctcg ccgcgctcgg cagagagcac tggccagggg tgcccggtgc ccatgttcga
     1621 cggcgcccag ggccgacctt cgagcgggca cgtgtggtcc ggcaggatat ggcagtcgcc
     1681 atacccgtcg ccgttgtagc gcaaccaccc cgagccgctc gccgattgct tctcgatggt
     1741 cttgtccacc accgtcagcg agttcagaac gtccgggtcg gtggccgaca gctcgccgag
     1801 gcgaaccagc tcgaggaatc cggcatcgat gaccgcgcgc tggtcgagcg tcgggccgcc
     1861 attgccgacg ttgtagctga tcgccgcatt cggatcaccg gtcttggaaa gccggatgaa
     1921 gtacggctga ctcgacaaag ggccgttcgt cgtgacggcc cagcccttga tggatcgctg
     1981 gtactggtcc gcggtcgcga gccagatccg ggcgtcggca ggagaagcgt gctgccgggc
     2041 gatcgttgcg gctgcgacca gcccggcgat ctcggccgcg attgttgacg gcgagtatcc
     2101 gctctgctcc tcccaacgct cgggcccgaa cgacggcccg tgcgcgatca aatagttcgc
     2161 cgccgcctgt acgtgcggcc acagtccggc gtcgccggcc aggccggact gcaacaccat
     2221 caggatcgga taggcaacct catccagttg ggtgttgaag gaatccggcg caagcttgcc
     2281 gttgaccaac gagttgcgtg gcaacgaccc gtcggggagc tgctgcttct cgagcaggaa
     2341 cctcgtggcg gcgcgggcgg tggccagatc gccgtccgtc aacagccccg tccatgcctc
     2401 atagaggtcc cgggagaaga cttcccggta ggagccgaag taggtgttgg ccgggtcgcc
     2461 ggcggagacc gcctgccccc acggcgaggc gatcgaggcc acgattgccc ctgggaatgt
     2521 cttgtcctcg ctcgctttga ctacgttggc ggacacgtag tactcattca ctgcggccgc
     2581 ctgctgggcc ggcgtgaggc cggggagact tccagatggg gtattgagcg cgtcgtcgta
     2641 tgaaagccac cccgaccggt attggccgcg cagcgacccg aagggccggc cgaccgtggc
     2701 gctggcggtg gcgacggcgt gggcctgcgt ggtgccgaaa ccgagggcga gcatcgcgtc
     2761 gccgtgcttg tcgaagcgca cctgcgccgt ctgctccaca ttgccgccga gagcggaatc
     2821 ggtcggcgtc aggatatgcg cggagtcgag ctgggcgagt ccatccgacg gggtgccgac
     2881 gaagccgctg ctcactacgc cgaacggttg gtcggcccgc agggccagga agctcggcac
     2941 ggcataatcg cgattggcgg cgtttgtctt ggtgttcgtg tcgtaggaga ccggcaccgg
     3001 cgaaccagtt gtggtgtcga tgaccgcgtt gtcggcaccg ccgttgccgc tgccgccgcc
     3061 accgttgccg ccgactgtcg cgtccagccg gacatagacc cggtagctgg ccgcggccga
     3121 cgtcaggggc gtgtagctgg tctggacaac aacgctcgga cgcgccggat cggtgaggta
     3181 cgttgtggta agccggtagg caccggtctt agccgtggac gtgactgtgc agaccattcc
     3241 ggtcgaatca gcggagacgg tgtaggtcgt gtcgcgcgtc tgcaggtcag tgaacgagga
     3301 gccgtcggtg acgaggtact gcatcgtctc gacgttcgtc gtgtcgatcg tcggggcata
     3361 gacatctgac agcacgccgt cggcgaccgt gtaccagatc ttcgacgtcg tgttgcgggc
     3421 cgtgcccaga cagtcctttc gggcgaggtc gaagtggctt aaggcgcctg ggcccccagt
     3481 ggcgggcccg ggggcggcga cagcggccgg aacagcgacc gcgagcgagc cggcgacaac
     3541 cgcgccgacc gccagaacgg cgaaccagga tcgtgcgcgc atggctctcc ttgttaagcc
     3601 ttgttaagcc cgatgccact gtctgccggc cactcagcca accgcatcag ccgggtgggg
     3661 tcaagggatt gtcgctgccc ctttgtgcgt gcgcctccaa atgacctggt gaccctggca
     3721 cgtgggtcgg cgaccaccca tatcagagcc gcggtgtgca gcggcgtgtc gatgcaggtt
     3781 ttcagacctc ggcgtcggtg ggcggggtgg cacgtcccgg cagcggcgtc ctgaccggca
     3841 ccagcccgag atactcgccg agatcgccga gcttgatctg acgtagctcc tcgtcgatgg
     3901 tgaccgaccc gcgccctctt gacacaactg gacgttctgt gcccgtcaac cgcggcgcct
     3961 cgtctccaac gcgaggtgca tactcggcag ggcggtcgcc gtcctgactg gccgctcgat
     4021 ccgttaccga gcgtgcaact gatctgcgct gtcctttctg gcgccgccgg tgacacgccg
     4081 ggacgggcga cggtcacgcg gtacaggcca ccgtccaccc tgccatccac ccggctgagc
     4141 gcgtcgcggg tcctcagaag cttgctgcca gagcatcaga tcatcgcacc gggacgcgcg
     4201 gacgtaccgg cggccataat gccgcgttca gcgaacagct gttcgccgat ggtgatgatc
     4261 tggccattgc cagtcacgtc atcctgccgg ggcaggtact gggcggcgga cccgcggatg
     4321 gtctcctggc gggtggtgcc cggtgaacat cggcgacccg gccctgaagc agccggccct
     4381 gctacgcggc agtgatctca aggtgctgcg agcgtccggc cggactgcgg acgcgttccc
     4441 ggaccggaaa ggctagcacg tagcggccga cacgccaggt ctccagctca tcgccatgat
     4501 caccgcgaca gagctgtggc gaggggcact tgaggggcac tttccggccc ggattatccc
     4561 cggttaaatt ggcgaaaaac acgtataacc tgcgagcccc gttacggaat cgaaccgtag
     4621 accttctcct taccatggac gctcatcccg tctggaaggg tcgcgctggg tcgcgtcag
//
LOCUS       DASWBF010000347         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_360814,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000347 DASWBF010000000
VERSION     DASWBF010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360814"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VF874_12805"
     CDS             complement(<1..1123)
                     /locus_tag="VF874_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9176909.1"
                     /translation="MFVDNNRVFYSASDLAAAARCPYALLREFDHKLGRGPAPELDDD
                     ELLRRTVELGDAHERRVLEQFVARHGDDVVRIGRPAYTDASFRAAARATVEALAREPA
                     VVCQAAVIDDRFVGFADFLVRDDGGAYQVRDTKLARHAKIEALLQIAGYAEALRAMGI
                     RVTPSAALILGDGSTVEYPVDEIVEVYRRQRAALQELLDRHLASGRPVKWADEDVRAC
                     MRCVVCEPQVCASDDLLLVAGLRASQRAKLLDAGIGTMTQLAEHTGPVADMAPSTLAT
                     LTAQARMQVRQQHSGGAEFEVIDADPLGALPDKDDGDLFFDFEGDPLWTANGVDWGLE
                     YLFGVLERDGTFHPLWAHDRPDERAALVTFLTMVRKRRKR"
     gene            complement(1195..1746)
                     /locus_tag="VF874_12810"
     CDS             complement(1195..1746)
                     /locus_tag="VF874_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppP/LprE family lipoprotein"
                     /protein_id="HEX9176910.1"
                     /translation="MLVVAAVTAATMAGCGASDSTVAKTPERTTTVAPTPPAQAAPPS
                     TPPPTPAPTAPAPVAYDPCATNLGAPEIARAVASLPPDPRSGQGWNPEPVAGNYNECA
                     QLSVVIVRANTNGDNPNTRALMFHQGKFIPTGVPDTFGFNGVDSSTSTGDTVALKFSN
                     GLGLDSVVRYRWNGNNVELVANA"
     gene            complement(2127..>2646)
                     /locus_tag="VF874_12815"
     CDS             complement(2127..>2646)
                     /locus_tag="VF874_12815"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9176911.1"
                     /translation="TQRKTDPGIDVPWSGFDLINGFNAWKITAADADGREVARVTDAP
                     KALAAGMKRQQRLAKSLARKQKGSGNRRDAAAKLGLHHHHVANVRRHFLHQVSGALVK
                     THDRLVIENLNVSGMLANPRLARAISDAGWAQFARLLGYKQAWRGGTILVADRWYPSS
                     RRCSACGPSTPV"
BASE COUNT          386 a          885 c          925 g          450 t
ORIGIN      
        1 agcgtttgcg gcgcttgcgc accatcgtca ggaacgtgac cagcgccgcg cgctcgtccg
       61 gacggtcgtg cgcccaaagc ggatggaagg tgccgtcgcg ctccagcacc ccgaacaggt
      121 actccaatcc ccagtcgacg ccgttcgcgg tccacagcgg gtcgccctcg aaatcgaaga
      181 acaggtcacc gtcgtccttg tcgggcagcg caccgagcgg gtcggcgtcg atcacttcga
      241 actcggcgcc gccggagtgc tgttggcgta cctgcatccg ggcctgcgcg gtgagcgtgg
      301 ccagcgtgct cggcgccatg tccgccaccg gcccggtgtg ttcggcgagc tgcgtcatcg
      361 tgccgatacc ggcgtccaac agtttggcgc gctggctggc ccgcaggccg gccaccagga
      421 gcaggtcgtc ggacgcgcaa acctgcggct cgcacaccac acatcgcatg cacgcgcgca
      481 cgtcctcgtc tgcccacttc actggccgcc cggacgcgag gtgccggtcg agtagttcct
      541 gcagcgccgc tcgctgacgc cgatatacct cgacgatctc gtcgacgggg tactcgaccg
      601 tcgacccgtc gccgagaatc aacgcggcgg acggcgtcac ccgaatgccc atggcccgca
      661 gcgcctcggc gtagccggcg atctgcagca gcgcctcgat cttggcgtgc cgggccagct
      721 tggtgtcgcg cacctggtag gcgccgccgt cgtcgcggac gaggaagtcc gcgaaaccga
      781 cgaaccggtc gtcgatcaca gcggcctgac acaccaccgc gggttcgcgc gccaacgcct
      841 cgaccgtggc gcgggcggcc gcacgaaacg acgcatccgt gtaggcgggc cggccgatcc
      901 ggaccacgtc gtcgccgtgg cgcgcgacga actgctcgag gacacggcgc tcatgcgcgt
      961 cccccaactc gaccgtgcgc ctcagcagtt cgtcgtcgtc gagctcgggc gccggtccgc
     1021 ggcccagctt gtggtcgaac tcgcgcagca gcgcatacgg gcacctggcg gcggccgcaa
     1081 ggtccgaggc gctatagaag acgcggttgt tgtcgacgaa cacggtgtca ctgtatggca
     1141 ggcctctgac atggttcttc gcgcaagcgg ctcatcaccg acaggttccg gtgctcaggc
     1201 gttcgcgacg agttcgacgt tgttgccgtt ccacctgtat cgaacgacgc tgtccagccc
     1261 gagtccgttg gagaacttca gtgccacggt atcgccggta ctcgtggacg agtcgacgcc
     1321 gttgaacccg aacgtgtcgg gcaccccggt gggaatgaac ttgccttgat ggaacatcag
     1381 cgcgcgggta ttcggattgt cgccgttggt gttggccctg acgatcacca ccgatagctg
     1441 cgcgcactcg ttgtagttcc cggcgacggg ctcggggttc cagccttggc cgctgcgggg
     1501 atcgggcggc aggctcgcga ccgccctggc gatctccggc gcgcccaggt tggttgcgca
     1561 cgggtcgtac gccaccgggg ccggagcggt aggcgcaggc gtcggcgggg gagtcgacgg
     1621 aggtgccgcc tgggctggcg gcgtcggcgc gacggtggtg gtccgttccg gcgtcttggc
     1681 gacggtcgag tcgctggcgc cgcatcccgc catggtcgcc gcggtgacgg cggccacgac
     1741 caacatccct agccgcctca cagttgggca cggtaccgcc ggccgctgcg atttcaccgc
     1801 aggcagggcc gacgatgcgc ggacactaaa gcgtgtcgaa caaaatctgt tgagtgttcg
     1861 gcaccgccct tctcgggcgt cttcggttca ggccgcgggt gggctgtgaa cgtcggttcc
     1921 cgcgtctttc gggctagttt caccggcaca cgtggggtgc tggtcagcgc cgtcccgtcg
     1981 gcgggcgttg gtggcccggc cccgttgctt gggggtccgg gggtcgaggt ggtgctgttg
     2041 cccccagcgg gcgaggttca ccgcggcgtt gcagtcgcgg tcggcggaat atccgcaacc
     2101 acaagtgaac acccgatcgg ccagcttcag accggtgtgg acggcccgca ggccgaacac
     2161 cgcctgctgg atgggtacca gcggtcggcg accaggatgg tgccgccgcg ccaagcctgt
     2221 ttgtagccca ggaggcgggc gaactgggcc cagccggcat cggagatggc gcgggccagg
     2281 cgagggttgg ccagcatccc ggacacgttc aggttttcga tgacgagccg gtcgtgggtc
     2341 ttgaccagcg cgcccgacac ctggtgcagg aaatggcggc gcacattggc gacatgatgg
     2401 tgatggagcc ccagcttggc ggcggcgtca cggcggttac cggatccttt ctgcttgcgc
     2461 gccaacgatt tcgccagccg ctgctggcgt ttcatcccgg cggccagcgc tttgggcgca
     2521 tcggtaaccc gagcgacttc gcggccgtcg gcgtcggcgg cggttatctt ccacgcgttg
     2581 aacccgttga tcagatcaaa ccccgaccac ggcacgtcga tacctggatc ggttttgcgt
     2641 tgggtg
//
LOCUS       DASWBF010000348        11873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1147901,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000348 DASWBF010000000
VERSION     DASWBF010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147901"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..745
                     /locus_tag="VF874_12820"
     CDS             380..745
                     /locus_tag="VF874_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176912.1"
                     /translation="MFDEHSNRPLYLGRTRIASADQRIVLYARDGGCTAPGCDKPGYE
                     CEVMHLRAHARDGLTEPDNLAFGCHTDHKLHDEGWTTRLNDDGVVEWVPPPHLNLKPG
                     INNFFHPQRYLPEDDEDDP"
     gene            complement(1165..1680)
                     /locus_tag="VF874_12825"
     CDS             complement(1165..1680)
                     /locus_tag="VF874_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176913.1"
                     /translation="MAATALLPGLSQINAWDTAHLGAAAASWTTTAETWEDAFTTVYR
                     QAPFPGGTAWEGDAADAAVLRVGSDRLTVLGAVDALHDAASVARSGASDIQAARQVVI
                     AAVREAQAAGFTVGEDLSVTSRHTGGPPALQAARRAQAEGLAATIRSRAAVLAAVEEQ
                     VASESQVRQPL"
     gene            complement(1681..1968)
                     /locus_tag="VF874_12830"
     CDS             complement(1681..1968)
                     /locus_tag="VF874_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176914.1"
                     /translation="MGSIQADVDGLSVLAANCEQRADVVSTVVGPPPVGPSFQASAAA
                     VLAAHDDITAAGNEFMARMQSTAADVTIAAHEYALTEAGSASAVSAIATKV"
     gene            complement(2035..2532)
                     /locus_tag="VF874_12835"
     CDS             complement(2035..2532)
                     /locus_tag="VF874_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX9176915.1"
                     /translation="MSTNQRGQIVMSDDEIDGFVASSRNGTLATIGPDGQPHLTAMWY
                     AVVDGEVWFETKAKSQKAVNLRRDPRITFLLEDGDTYDTLRGVSFEGTAEIVDDPDAI
                     FRVGVSVWERYTGPYTDEVKPAVDMMMNKRIAIRLAVKRVRSWDHRKLGMPPMPLAGS
                     TAPAL"
     gene            complement(2553..3254)
                     /locus_tag="VF874_12840"
     CDS             complement(2553..3254)
                     /locus_tag="VF874_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9176916.1"
                     /translation="MRQDSGIGVLDKAVGLLRTVAESPCGLAELCERTGLPRATAHRL
                     AAGLEKHRLLARDGQGRWRLGPAVSELAGQVHDPLLAAGTAVLPRLREITGESVQLYR
                     REGTWRVCVAAMEPPAGLRDTVPVGTRLPMTAGSGAKVLLAFSDAATQQAVLPAAKFT
                     ERTLAEVRRRGWAQSAAEREPGVASVSAPVRDGRGDVIAAVSVSGPIDRMGRRPGARW
                     AADLLAASEALTRRL"
     gene            3311..4714
                     /gene="leuC"
                     /locus_tag="VF874_12845"
     CDS             3311..4714
                     /gene="leuC"
                     /locus_tag="VF874_12845"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX9176917.1"
                     /translation="MAEKVWDSHVVARGAGAGASREPDLIYIDMHFIHEVTSPQAFDG
                     LRLARRTVRRPDLTIATEDHNVPTVDIDKPIADPISRTQVETLRHNCAEFGIRLHPMG
                     DVEQGIVHVIGPQLGLTQPGMTIVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLP
                     LRPFKTMAVNVDGELPPGVTAKDIILAVIAKIGTGGGQGYVIEYRGSTIESLSMEGRM
                     TICNMSIEAGARAGMVAPDETTYEYLRGRPHAPSGAQWEIAVAEWDRLRTDDGAEFDT
                     EVYVDAATLSPFVTWGTNPGQGVPLSEAVPDPELMTDEGQRQDAEKALTYMDLRAGTP
                     MRDVAVDAVFVGSCTNGRIEDLRVVADVLRGRKVADGVRMLIVPGSMRVRAQAESEGL
                     GEIFTAAGAEWRQAGCSMCLGMNPDQLSPGERCASTSNRNFEGRQGKGGRTHLVSPAV
                     AAATAVRGTLSAPADLN"
     gene            4738..5334
                     /gene="leuD"
                     /locus_tag="VF874_12850"
     CDS             4738..5334
                     /gene="leuD"
                     /locus_tag="VF874_12850"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9176918.1"
                     /translation="MEAFRTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFDDGLFA
                     TWRTDPNFVLNLAPFDKGSVLVAGPDFGTGSSREHAVWALMDFGFRVVISSRFADIFR
                     GNAGKAGLLAAEVAQPDVELLWKLIEQTPGLEITVNLQDRTVAAGTVVVPFAIDDYTA
                     WRLLEGLDDIGLTLRKLDPIEDFEAARPEWKPTTSTAI"
     gene            5534..6127
                     /locus_tag="VF874_12855"
     CDS             5534..6127
                     /locus_tag="VF874_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9176919.1"
                     /translation="MNKAELIDELTKKLGADRRQATAAVENVVDTIVRAVNRGESVTI
                     TGFGVFEQRRRAARVARNPRTGETVKVRPTSVPAFRPGAQFKAIVAGAQRLPSEGPAV
                     KRGSGAGGGTVRRAAAKKAVKRQAVKKAVKRQAVKKAVTKTAAKKAVKKTAAKKAVKR
                     TAVKRTAVKKAAVKRAATKAPAKKTAAKKAPAKRGRR"
     gene            complement(6177..7070)
                     /locus_tag="VF874_12860"
     CDS             complement(6177..7070)
                     /locus_tag="VF874_12860"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9176920.1"
                     /translation="MTKPILASGAVLWRGVEGSSDVEVAIIHRPRYDDWSLPKGKVDP
                     GETESAAAVREIYEETGQRAYLGRALGTVNYPITLGVKHVRYWAARAIGGDFVPTAEV
                     NDMVWLSAADAMGKVSYPDDRKVLRRFTKLPADTKTVLIVRHGTAGSKKRYKGDDRNR
                     PLDENGRAQAKLLVSQLLAFGPTSLYAADRVRCVQTIKPLAGELGAEITVEPTLTEEA
                     YWQDRDTARQRVLEIAAGDGTPVISTQGKVIPDLIQWWTERAGIRPAKSRNRKGSTWV
                     LSLADGKLVSADHIASPVPPE"
     gene            complement(7070..9286)
                     /locus_tag="VF874_12865"
     CDS             complement(7070..9286)
                     /locus_tag="VF874_12865"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX9176921.1"
                     /translation="MIDEVTETEAEVSTQTEWPPSARRESTGSTPEAPPAATAEEVVD
                     ALPEDRYLNRELSWLDFNARVLALAADTSLPLLERAKFLAIFASNLDEFYMVRVAGLK
                     RRDEMGLSVRSADGLSPREQLRRIGELTQKIAGRHARVFLDSVRPALADEGIVIVTWD
                     DLDDVERSELSTHFHEQVFPVLTPLAVDPAHPFPFVSGLSLNLAITVRHPADSGQHFA
                     RVKVPDNVDRFVELGSRQGAGAAVRFLPMEELIAAFLPVLFPGMEIVEHHAFRITRNA
                     DFEVEEDRDEDLLQALERELARRRFGSPVRLEVSDDMTEHMLELLLRELDVDPSDVID
                     VPGLLDLSSLWQIYGVDRPALKDPPFVPATHPAFGERETPKSIFATLQDGDVLVHHPY
                     HSFSTSVQRFIEQAAADPSVLAIKQTLYRTSGDSPIVNALIDAAEAGKQVVALVELKA
                     RFDEQANIKWARALEQAGVHVVYGLIGLKTHCKTCLVVRREGSVIRRYCHVGTGNYNP
                     KTARLYEDIGLLTAAPDIGADLTDLFNSLTGYSRKVTYRNLLVAPYGVRRGIIERIDR
                     EIEATRAGAGAAGSATSHTEGRIRIKANALVDEQVIDALYRASRAGVRVEVVVRGICA
                     LRPGAAGFSENITVRSILGRFLEHSRIFHFGAIDEFWIGSADMMHRNLDRRIEVLAQV
                     KDPKLTAQLNEIFESALDPATRCWELGSDGKWTAAPHAGHTVRDHQVSLMERHRHR"
     gene            complement(9360..10001)
                     /gene="cofC"
                     /locus_tag="VF874_12870"
     CDS             complement(9360..10001)
                     /gene="cofC"
                     /locus_tag="VF874_12870"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX9176922.1"
                     /translation="MGGEVGLIIAVKRLAVAKTRLAPVFSAPTRERVVLAMLLDTITA
                     AAKEPSLRGITVVTPDDVAAEAARQLGADVLADPTPDGHPDPLNNAIVAAETVVGSAP
                     DPPSNVIVLQGDLPALQTQELTQAIAAARAHRRSFVADRHGSGTVALFAFGSPLEPAF
                     GPDSARRHRHSGAVELTGPWPGMRCDIDTPEDLQAARRLGVGPATAQAIARRM"
     gene            10046..11044
                     /locus_tag="VF874_12875"
     CDS             10046..11044
                     /locus_tag="VF874_12875"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX9176923.1"
                     /translation="MAEVAVMGAGSWGSALAQVLADSGNSVTLWARSADVADEINTDH
                     RNRRYLGHAQLPTEVRATLDPAKALTGVTTVLLAVPSQVMRANLSHWTDLIEDDATLV
                     SLAKGIEIDTLMRMSQVVAQVTGVDSSRVAVLSGPNLASEIVERQPAATVIACSDSGR
                     AVALQRMLSAGYFRPYTNADVVGVEIGGACKNVIALACGMAAGVGLGENTAAAIITRG
                     LAEMMRLGIALGAKPATLAGLAGVGDLVATCTSPYSRNRTFGERLGRGGTMESALEAA
                     GGHIAEGVTSCRSVLALASSYDVEMPLTDAVNQVCHHGLSVTDAIAFLLGRRPKPE"
     gene            11051..>11873
                     /locus_tag="VF874_12880"
     CDS             11051..>11873
                     /locus_tag="VF874_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX9176924.1"
                     /translation="MPGPYGDSTRCLKAVGVQAVPGQPVAPVPVPASTYHLSSDEFEE
                     LDTYGRSSNPTWRQLESAIAQLEGATAALVFGSGMAAITSVLRALVRPRGVLVVPADG
                     YYQVRRYAVEYLARQGITVREATAGQMCEAAADADVVLAETPVNPQLDVVDLHRLALM
                     CRSRGATLVVDNTAATPLGQQPLGLGADLVVASATKALAGHSDLVAGYVAGSKPELIA
                     AVAAERLLTGPILGAFEAWLVLRSLGTAGLRFERQCRNAQALATMLHSHPAVRSVR"
BASE COUNT         2028 a         3921 c         3909 g         2015 t
ORIGIN      
        1 gaggcccact cccccgcctc cggcgccgga ccgaacagtc cgcgtgcatc gccaagtgcc
       61 tggccaacct cggccaccag cgcatccagc gacatggcac gcagtatcac ccaccaccca
      121 cgaaaccggc caccaccacc aacaacaact cggagggcac tgtcaacttg aggtcgtgtg
      181 gacgttctgc taaccacata agcctcatac gtcacacgcg acctggtgaa tcgcggaggc
      241 gaaccggcga aaatcgccct cggaattgct caagttgaca gtgccgtggc tgacgagttc
      301 atcgacctgg atggtctgtt cgacgagttg acccggatac ggacgtaggc agcgctgtgg
      361 atgactatct cttttggcgg tgttcgacga gcactccaat cggccgctgt atctgggccg
      421 cacccgcatc gcctccgcgg atcagcgcat cgtgctctac gcgcgcgacg gcggctgcac
      481 cgccccagga tgcgacaaac ccggctatga gtgcgaagtc atgcacctgc gcgcccatgc
      541 ccgcgacggg ctcaccgaac ccgacaacct ggccttcggg tgccacaccg accacaaact
      601 gcacgacgaa ggctggacca cccggctcaa cgacgacggc gtcgtcgaat gggtgccccc
      661 gccgcacctg aacctcaaac ccggcatcaa caacttcttc cacccccagc gctacctacc
      721 cgaagacgac gaagacgacc cgtagcgccg tagaaccgag ctcgacattc aagcgactca
      781 gcggcggtga gttgcccgag ttccgcgcgc cgatggaact ttcgtgcgct gaccgagcgc
      841 attaaatgag tgccgggcac tgtacgttcg cgcaccaggc tgagtcctag gttggccggg
      901 gtgcatctac tgcattgcgg gtgaggtagt cgaacatcgc gcgaatctcg tcggccgtcg
      961 gaagtgttac tacgggatcg tttcggcccg gctcaagttt gtcgagtacg gccctgacgt
     1021 cctgggcggt tttgatcggc ttggtgtctg gatcgtctgg tgtgttcggc ggtggttcct
     1081 gagggaaggt gcggttgtcg acggcctgga ttgacggttt ctctttccgc ggtacgaccg
     1141 ggttcggagc gaattggatg ccgctcaaag aggctgtcgc acttgtgatt cactcgcgac
     1201 ctgttcctcg accgcagcca ggaccgccgc tcggctgcga atggtcgcgg ctagtccttc
     1261 ggcctgcgct cggcgcgccg cctgcaatgc gggcggccca ccggtgtgtc tgcttgtgac
     1321 cgaaagatcc tcgccgaccg tgaaccccgc cgcttgcgcc tcgcgtaccg ctgcgataac
     1381 cacttgtctg gctgcttgaa tgtcgcttgc ccccgaccgc gcgacgcttg cggcgtcatg
     1441 caatgcgtcg accgctccca gcaccgtgag ccggtcgcta ccgacccgca gcaccgccgc
     1501 atcggcggcg tcgccttccc acgcagtccc gccgggaaac ggggcttgcc gatataccgt
     1561 cgtgaatgcg tcctcccacg tctcagcggt cgttgtccaa gaagctgcgg ctgcgcccag
     1621 atgtgcggtg tcccacgcat tgatctgcga cagccccggt agcagcgcgg tcgcagccac
     1681 ctataccttc gtcgctatcg cggacacggc agacgctgaa cccgcctcgg tcaaggcgta
     1741 ttcatgagca gcgatggtga cgtcggcggc cgttgattgc atgcgcgcca tgaactcgtt
     1801 tccggcggcg gtgatgtcgt catgcgcggc gagtacggct gcggcgcttg cctgaaatga
     1861 ggggccgaca ggcggcggcc cgaccacagt gctaaccaca tcggctcgct gctcacaatt
     1921 ggcggccaac accgacagcc cgtcgacatc ggcctgaatc gaccccacaa atcgacccta
     1981 agcccgcctg agcacccgcc ttcgcgcgaa tttgtcgccg aaactgcgac gcggtcagag
     2041 ggcgggtgcc gtcgaccccg ccagcggcat cggcggcatc ccgagcttgc ggtgatccca
     2101 ggagcgaaca cgcttaaccg ccaaccgaat tgcgatccgc ttgttcatca tcatgtccac
     2161 cgccggcttg acctcgtcgg tatacgggcc cgtgtagcgt tcccacacgc tgacgccgac
     2221 gcggaagatc gcgtccggat cgtcgacgat ttcggcggtg ccctcgaacg acaccccgcg
     2281 cagcgtgtcg taggtgtcgc cgtcctccag caggaacgtg atgcgcgggt cgcggcgaag
     2341 attgacagcc ttctgcgact tggctttcgt ctcgaaccag acttcgccgt ccacgacggc
     2401 gtaccacatc gcggtcagat gcggctgccc gtctggtccg atcgtggcca aagttccgtt
     2461 gcggctcgac gcgacgaacc cgtcgatctc gtcgtccgac atcacgatct gcccccgttg
     2521 attcgtgctc atggccgtag tctctcaggc catcagagcc tgcgtgtcag tgcctccgag
     2581 gccgccagca gatccgccgc ccagcgcgca ccgggccgac gacccatccg gtcgatcggc
     2641 cccgacacgg atacggcggc aatcacatcc ccgcgcccgt cgcgcaccgg cgccgagacg
     2701 ctcgccacgc ctggctcgcg ttccgcggcg ctctgcgccc acccgcgacg gcgtacctcg
     2761 gccagcgtcc gctcggtgaa cttcgcggcg ggcaggacgg cctgctgggt ggccgcatcg
     2821 ctgaacgcca gcaacacctt ggcccccgag cccgccgtca tcggtagccg cgtgcccacc
     2881 ggcaccgtgt cacgaaggcc cgcaggcggt tccatcgcgg cgacgcacac ccgccacgtg
     2941 ccctcccggc ggtacagctg cacgctctca ccagtgatct cgcgcagcct cggcagcacc
     3001 gccgtcccgg cggccagcag cgggtcgtgc acctgccccg ccaactcgct caccgcgggt
     3061 cccagccgcc accggccctg cccgtcgcgg gccagcagcc gatgcttctc gagtccggcg
     3121 gccagccggt gggcggtcgc gcggggcagg cccgtccgct cgcacaactc agccagcccg
     3181 cacggggact cggccaccgt gcgcaggaga cccaccgctt tgtcgagcac gccgatgccg
     3241 ctatcctgtc tcatatcgag atactaactt cccagattgt gagacaccag tgatagccac
     3301 cccgcgcacc atggcggaaa aggtatggga ctcgcatgtg gtcgcccgcg gcgcgggcgc
     3361 cggggcgtcg cgcgaacccg atctgatcta catcgacatg cacttcatcc acgaggtcac
     3421 cagtccgcag gcgttcgacg gactacgcct tgccaggcgc acggttcgac ggccggacct
     3481 gacgatcgcg accgaggacc acaacgtgcc gacggtcgac atcgacaagc cgatcgccga
     3541 cccgatttca cgcacccagg tcgagacgtt gcgccacaac tgcgccgaat tcggtatccg
     3601 cctgcatccg atgggcgacg tcgagcaggg catcgtccac gtcatcggac cgcaactggg
     3661 tctgacgcag cccggcatga ccatcgtctg cggcgacagt cacacatcca cgcacggtgc
     3721 attcggcgcg ctggcgatgg gtatcggcac atcggaagtc gagcatgtcc tggccaccca
     3781 aacgttgccg ttgcggccgt tcaagacgat ggcggtgaac gtggacggcg aactgccgcc
     3841 gggtgtcacg gccaaggaca tcatcttggc ggtgatcgcg aaaatcggaa ccggcggcgg
     3901 tcagggttac gtcatcgaat accgcggcag caccatcgaa tcactgtcga tggaaggccg
     3961 gatgacgatc tgcaacatga gcatcgaggc gggcgcccgt gcgggcatgg tggcgccgga
     4021 cgagacgacc tacgaatatc tgcgcggccg tccgcacgcg ccgtcggggg cgcagtggga
     4081 gattgcggtc gccgaatggg atcggctgcg caccgacgac ggcgcggaat tcgacaccga
     4141 ggtgtacgtc gacgccgcga cgttgagtcc gttcgtcacc tggggaacca atcccggaca
     4201 gggtgtgccg ctttccgagg cagtgcccga tcccgaactg atgaccgacg agggtcagcg
     4261 gcaggatgcc gaaaaggcgt tgacctacat ggatcttcgg gccggaacgc cgatgcgtga
     4321 cgtcgctgtg gacgcggtgt tcgtcggctc gtgcacgaac gggcgcatcg aagatctgcg
     4381 tgtggtcgcc gacgtgttgc gcggccgcaa agtcgccgac ggcgtgcgga tgctgatcgt
     4441 gcccggctcg atgcgggtgc gcgcccaagc cgaatcggaa ggactgggcg aaatcttcac
     4501 cgctgcaggc gcggagtggc ggcaggccgg atgctcgatg tgcctgggca tgaacccgga
     4561 tcaactgtcg ccaggggagc ggtgcgcgtc gacgtctaac cgcaacttcg agggccggca
     4621 gggcaagggc gggcggaccc atctcgtgtc gcccgctgtt gcggccgcaa ccgccgtgcg
     4681 cggcacactg tcggcacccg cggacctgaa ctgaattctt gttaggagat atagacaatg
     4741 gaggcttttc gtacgcacac cgggatcggt gttccgctgc ggcggtccaa tgtcgacacc
     4801 gaccaaatca tccccgccgt ctatctgaag cgggtgaccc gaacgggatt cgacgacggg
     4861 ctattcgcga cctggcgtac agacccgaat ttcgtattga accttgcccc gttcgacaag
     4921 ggttcggtgt tggtcgctgg acccgatttc ggcaccggat cctcacgcga gcacgcggtg
     4981 tgggcgctga tggactttgg gttccgggtg gtcatctcgt cgcgattcgc cgacatcttt
     5041 cggggcaacg ccggcaaggc gggactcttg gcggccgaag tggcccaacc cgatgtggaa
     5101 ctgctttgga agctgatcga acagactcca ggcttggaaa tcactgtcaa tcttcaagat
     5161 cggacagtgg cagccggaac cgtggtggtg ccgttcgcga ttgacgatta caccgcctgg
     5221 aggcttctcg aaggactcga cgatataggc cttacgctgc gaaaactcga cccgatcgag
     5281 gatttcgaag cagctcggcc ggagtggaaa ccgaccactt cgacggcgat ctgaccgatc
     5341 aagatcaccc aattctgggg ctgtcagcgc cgtttcggca ctcgcggacc acctaccaag
     5401 cgcggcaagc agattgccga taacgcgatc gaattcgatt gaaattgcgc gtggctcttg
     5461 gaaatcagca catcaagggt ttaccgtgtc ctctagtcgg tccagaaagg accgctggct
     5521 tcggaggatt tgcatgaaca aagcagagct catcgacgaa ctcacaaaga aactgggcgc
     5581 ggatcgtcgg caagcgacgg cggcggtcga gaacgtcgtc gacaccattg tgcgcgcggt
     5641 gaaccgcggt gagagcgtca ccattacggg cttcggcgtt ttcgaacagc gccgccgtgc
     5701 tgctcgcgtg gcgcgcaatc cgcgcaccgg cgaaacggtg aaagtcaggc caacgtctgt
     5761 cccggcgttc cggccgggtg ctcagttcaa ggcaattgtg gcaggggcgc agcgtcttcc
     5821 gtccgagggc ccggcggtga agcgcggctc gggcgcgggc ggtggcaccg tcaggcgggc
     5881 cgctgccaag aaggctgtca agcggcaggc tgtgaagaag gctgtcaagc ggcaggctgt
     5941 gaagaaggcc gtcacgaaga ctgccgccaa gaaggccgtc aagaagactg ccgccaagaa
     6001 ggccgtcaag aggactgccg tcaagaggac tgccgtcaag aaggctgccg tcaagcgcgc
     6061 ggccaccaag gccccggcca agaagacagc ggccaagaag gccccggcca agcgcggccg
     6121 ccggtaattt cgacgacact taaccgccgc gagtgaaacc gctcgcggcg gttaagtcat
     6181 tccggaggca ccgggctggc gatgtggtcg gcgctgacga gtttcccgtc ggccagcgac
     6241 agcacccacg tactgccttt gcggttgcgg gacttggccg gtcgaatccc ggcgcgctca
     6301 gtccaccact ggatcaagtc gggaatgacc ttcccctgcg tggagatcac cggagttccg
     6361 tctcctgctg cgatctccag cactcgctga cgcgccgtgt cgcggtcttg ccaatacgcc
     6421 tcctcggtca gcgtcggttc cactgtgatc tcggcgccaa gctcgcctgc aaggggcttg
     6481 atcgtctgaa cgcagcgcac ccgatccgcg gcgtacaacg acgtcggccc gaacgccagc
     6541 agttggctga cgagcaactt tgcctgggcc cgaccgttct cgtccagcgg tcgatttcgg
     6601 tcgtcgccct tgtaccgctt cttgctgccc gctgtgccgt ggcgcacgat caacaccgtc
     6661 ttcgtgtcgg cgggcagttt ggtgaaccgc ctcaacacct tccggtcatc cggataggac
     6721 accttgccca tggcgtcggc ggccgatagc cataccatgt cgttcacctc cgcggtgggc
     6781 acgaagtctc caccgatcgc gcgggccgcc caataccgta cgtgcttgac gcccagcgtg
     6841 atcggatagt tcaccgtgcc gagcgctcgg ccgagatatg cgcgctgtcc ggtttcctcg
     6901 tagatctcgc gcaccgccgc cgccgattcc gtctcacccg ggtccacttt gcccttgggc
     6961 agcgaccagt cgtcataccg ggggcggtgg atgatcgcca cctcgacgtc cgacgagcct
     7021 tcgacaccac gccacagcac agcgccggac gccaggatcg gtttggtcat cagcggtgcc
     7081 gatgccgctc catcagcgag acttggtgat cgcgcacggt gtgaccggca tgaggtgccg
     7141 cggtccattt accgtcggac ccgagttccc agcagcgcgt ggccggatcg agcgccgatt
     7201 cgaagatctc gttgagttgc gccgtcaact tcggatcctt gacctgagct agcacttcga
     7261 tccgccggtc caggttgcgg tgcatcatgt cggcgctgcc gatccagaac tcgtcaatcg
     7321 cgccgaagtg gaagatccgc gagtgttcaa ggaagcggcc gaggatcgaa cgaaccgtga
     7381 tgttctccga aaacccggca gcacctgggc gcagcgcaca aatgccgcgc accaccacct
     7441 cgacgcgcac gcccgctcgc gacgcccggt acagcgcgtc gatgacctgt tcgtcgacaa
     7501 gggcgttggc cttgatccgg attcggcctt cggtgtggga tgtcgccgac ccagcggctc
     7561 cggcgccggc gcgggtggcc tcgatctcgc ggtcgatccg ttcgatgatc cccctgcgga
     7621 cgccgtaggg agcgacgaga agattacggt acgtgacctt cctggaataa ccggtcagcg
     7681 agttgaagag atcggtcaag tcggcgccga tgtcgggcgc ggcggtgagc agaccgatgt
     7741 cctcatacag tcgtgcggtt ttcgggttgt agttaccggt gccgacatgg cagtagcgcc
     7801 ggatgaccga tccttcgcgc cgtaccacca ggcatgtctt gcagtgcgtc ttcagcccga
     7861 tgagcccgta cacgacgtgt acacccgctt gctccaacgc gcgtgcccac ttgatgttgg
     7921 cctgctcgtc gaagcgggcc ttcagttcga ccagcgcgac cacttgcttg ccggcctccg
     7981 ccgcgtcgat caacgcgttg acgatcggcg agtcgccgga cgtgcggtac agagtctgct
     8041 tgatcgccag cacacttggg tccgcggcgg cctgctcgat gaagcgttgc acgctggtgg
     8101 agaacgagtg gtacgggtgg tgcaccaaca catcaccgtc ctgcagtgtc gcgaagatgc
     8161 tcttaggcgt ctcacgctca ccgaacgcgg gatgcgtcgc cggtacgaac ggcggatcct
     8221 tcagcgccgg ccggtccacg ccgtagatct gccacagcga ggacaagtcc agaaggcctg
     8281 gtacgtcgat cacgtcgctg ggatccacgt ccagttcgcg cagcagcagc tcgagcatgt
     8341 gctcggtcat gtcgtcggac acttccagcc gcaccggaga tccgaaccgg cgccgggcca
     8401 gctcccgctc gagtgcctgc aacaggtcct cgtcgcggtc ttcctccacc tcgaaatcgg
     8461 cgttgcgcgt gatgcggaac gcatggtgct cgacgatctc catgcccggg aacagcacgg
     8521 gtaggaacgc cgcgatcagc tcctccatcg ggaggaaccg cacggctgcg ccggcacctt
     8581 ggcggctgcc gagctcgacg aaccgatcga cgttgtcggg caccttcact cgcgcgaagt
     8641 gctggccgct gtcggcggga tgtctgacgg tgatcgcgag gttcaggctc aacccgctga
     8701 cgaacggaaa cgggtgggcc ggatccaccg ccaggggcgt cagcaccggg aacacctgct
     8761 cgtggaagtg cgtcgacaac tccgaccgct cgacgtcgtc gaggtcgtcc cacgtgacga
     8821 tcacgatgcc ctcgtcggcc agcgccggtc gcaccgagtc caggaacacc cgggcgtggc
     8881 ggccggcgat cttctgggtc agctcaccga tgcggcgcag ttgttcacgc ggggacagcc
     8941 cgtcggccga gcgcaccgac agccccatct cgtcgcggcg tttgaggccg gccacccgca
     9001 ccatgtagaa ctcgtcgagg ttggacgcga agatcgccag gaacttggcg cgttccagca
     9061 gcggcagcga cgtgtcggcg gccagcgcca acacgcgggc gttgaagtcg agccaactga
     9121 gttcgcggtt gaggtaccgg tcctccggca acgcgtcgac gacctcttcc gccgtggcgg
     9181 cgggcggcgc ctcgggcgtc gaacccgtgc tctcccgccg ggcggacggg ggccactcag
     9241 tttgggtact gacttcggct tcggtctcgg tcacctcgtc gatcattcct catcggcggc
     9301 ccgaccggct acttaccggt gaagacgtga cgatcggcca cccgctgttg gatcaccgtt
     9361 cacatgcgcc gagcgatggc ctgtgcggtg gctgggccga caccgagtcg acgcgcagcc
     9421 tgcaggtctt cgggagtgtc gatatcgcac cgcatgccgg gccacggccc ggtgagttcc
     9481 actgcgcccg aatggcggtg tcgccgagcc gaatccggcc cgaacgcggg ttccaacgga
     9541 cttccgaagg cgaacagagc cacggttccg ctgccgtgcc ggtcggcgac aaagctgcgt
     9601 cggtgcgcgc gcgccgcggc gattgcctgc gtcagctcct gggtttgcag ggcgggcaaa
     9661 tcgccttgca gcacaatgac attcgacgga gggtcaggag cagatccgac gacggtttcg
     9721 gcggcgacga tcgcgttgtt gagcggatcc gggtggccgt ccggtgtggg gtcggcgagc
     9781 acgtccgcac cgagctggcg tgcggcctcg gcggcgacgt catcgggcgt caccacggtg
     9841 atgccgcgca gcgacggttc cttcgccgcc gcggtaatgg tgtcgagcag catcgcgagc
     9901 accacccgct cgcgggtggg cgccgagaat accggcgcga gccgagtctt ggcgaccgcg
     9961 agccgtttga cggcgatgat caggccgact tcaccgccca tggaggtcat cctgccagcc
    10021 caggtcgggc ggctagggtg aggcggtggc cgaggtcgcg gtgatgggcg caggatcgtg
    10081 gggcagcgcg ttggcccaag tgctcgccga ttccggcaac tcggtgacgc tgtgggcgag
    10141 aagcgccgac gtcgccgacg aaatcaacac cgaccaccgc aaccgccgct acctggggca
    10201 tgcgcagctg cccacagagg ttcgggcgac cctcgatccg gcgaaggcgc tgaccggcgt
    10261 cacgaccgtg ctgctcgcgg tgccgtcgca ggtgatgcgg gccaacctca gccactggac
    10321 cgacctgatc gaggacgacg cgacgctggt gagcctcgcg aaaggcatcg agatcgacac
    10381 gctgatgcgg atgagccagg tcgtcgcgca agtcacaggt gtcgattcat cccgcgtcgc
    10441 cgtgctgtcg gggccgaacc tggccagtga gatcgtcgag cgccaacccg ctgcgacggt
    10501 catcgcgtgc agcgattccg gccgggcggt ggcgctgcag cgcatgctga gcgccggcta
    10561 cttccgtccg tataccaacg ccgacgtggt cggggtagag atcggtggcg cgtgcaagaa
    10621 cgtgatcgcg ctggcgtgcg gaatggcggc cggggtgggg ctcggcgaga acaccgcggc
    10681 ggccatcatc acccgcggcc tggccgagat gatgcgactg ggtatcgcgc tgggcgccaa
    10741 gcccgcgacg ctggcggggt tggcgggtgt cggcgatctg gtggccacct gcacgtcgcc
    10801 gtattcgcgt aaccgcacgt tcggtgaacg gctgggcagg ggcggcacga tggaatctgc
    10861 gctggaggct gcagggggtc acatcgcgga gggtgtgacg tcgtgccggt cggtgctggc
    10921 gctggcttcc agttacgacg tcgaaatgcc gctgacggat gcggtgaatc aggtctgtca
    10981 ccatgggctt tcggtgaccg acgcgatcgc gttcctgctg gggcgacgcc ccaagccgga
    11041 gtgagcgaca gtgcccggtc cgtacggtga ttccacccgc tgcctgaaag ctgttggcgt
    11101 gcaggcggtt ccagggcagc cggtggcgcc ggtcccagtg ccggcgtcga cgtatcacct
    11161 gtcgagcgac gaattcgaag agctggatac ctacggccgg agctcgaacc ccacatggcg
    11221 gcaactcgag tcggccatcg cccaactcga gggtgcgacg gcggcgcttg tgttcggctc
    11281 ggggatggcg gcgatcacgt cggtgctgcg agcgctcgtc aggccgcgtg gggtccttgt
    11341 ggtgcctgcc gacggttact accaggtccg ccgctatgcg gtcgaatact tggcacgcca
    11401 aggaattaca gtgcgcgagg cgaccgccgg ccagatgtgc gaagcggcgg ctgacgccga
    11461 cgtggtgctc gccgagacgc cggtgaaccc gcagctcgat gtcgtcgatc tgcaccggtt
    11521 ggcgctgatg tgtcgcagcc gcggggcgac gctcgtcgtc gacaacaccg cagcgacgcc
    11581 gctgggtcag cagccgctgg ggctcggggc cgatctggtg gtggccagcg cgacaaaagc
    11641 gttggccggg cacagcgatc tcgtcgcggg ttacgtcgcc ggcagcaagc cggagctgat
    11701 agccgcggtc gccgccgagc gcctgttgac cgggccgatt ctcggtgcgt tcgaagcctg
    11761 gctggtgctg cgcagcctcg gcaccgcggg gctgcggttc gagcgccaat gccgcaacgc
    11821 gcaagcgctg gccacgatgc tgcatagtca tccggcggtg cgctccgtga ggt
//
LOCUS       DASWBF010000349         9024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_882504,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000349 DASWBF010000000
VERSION     DASWBF010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882504"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1341
                     /locus_tag="VF874_12885"
     CDS             151..1341
                     /locus_tag="VF874_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX9176925.1"
                     /translation="MVGPGLLAGLSDDDPAGITTYLVLGANYGYQLLWVLLLSTVALV
                     IFHGLAARMGVVTGQGLIGLVRQRYGVRLGGAVLAALIVANIGTTCAEFAGIAAGFEL
                     FGITRYLSVPAAAVIVSLLVLRGSFHRVEHFLLLLSTVFLAYIVSGVLAHPDWGAAVH
                     GTLVPTMTPGTESIAIVTATLGTTLAPWGLSFIQSYAVDKKLRTEDLPAERVDVVTGA
                     VLTGVIGFFVVVACAATLYRDGQPINDAADAARALEPLAGAAASQLFAVGLIGSAFLA
                     ASILPLSTAYSVCEYAGVEAALDDPFREARTFYLTFGIVTVLGAVAVLIPNVPLVTVL
                     VATQVLNAVLLVPLLVAMVGIGRDRDLMGRFRIGRAGTLAYGVTTAAVVACVAALAVT
                     SLID"
     gene            1366..2649
                     /locus_tag="VF874_12890"
     CDS             1366..2649
                     /locus_tag="VF874_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HEX9176926.1"
                     /translation="MADIVPIELALDDRDVFTLYAPRWRDASDEWEAFLGKEDELYAF
                     ESVPDLVAFVRSSADNDLTDHPAWETLTTASAHRLKPKNDKRFDLVNVYELLTEKPTQ
                     DSVEQLAGTLAIASAIGSVCELPAISKFFNGNPVLSSVSGGIADFSDRAGLRRWNEIG
                     AVVGKSWDKVIDAIEDVISTPEVDSKASVKAEAELDEPAPEEDEDEGVEGGEDEGEGG
                     EAAVADTETDESEEPTKAPAGDVVLGADEDFWGKVGIDPIRMMTSSGTFYTLRCYLND
                     EPIFLGRNGRISVFGSERALARYLADEHDHDLSDLATYDDIRTAATDGSLRVDVNDEN
                     VYVLTGIADDLADGPDAIDREQLGLAVELLRDVGDYSEDSTVDEVLEPDMPLGKLVAY
                     VLDPDTARKPGPPYAKAVEQWSELDSFVESRLRQE"
     gene            complement(2653..3741)
                     /locus_tag="VF874_12895"
     CDS             complement(2653..3741)
                     /locus_tag="VF874_12895"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX9176927.1"
                     /translation="MTTPLNLDMIGQAPKALLHDHLDGGLRPATVLELAEQVGYENLP
                     ATDADELAAWFRTAAHSGSLERYLEPFAHTVGVMQTPAALYRVAYECVEDLAADNVVY
                     AEVRFAPELHIAEGLSLDDVVDAVLAGFADGEKAVRADGRTITVRCLVTAMRHAARSL
                     EIAELAIRFRDKGVVGFDIAGAEAGHPPTRHLDAFEYMRNHNGRFTIHAGEAFGLPSI
                     HEAIAFCGADRLGHGVRIVDDISENPDGTYTLGRVAGIVRDKRIPLEMCPSSNVQTGA
                     VRSIAEHPFDLLARLRFRVTVNTDNRLMSDTTMSQEMLRLVEAFGYGWSDLARFTINA
                     MKSAFIPFDERLAIIDDVIKPRFAVLIG"
     gene            complement(3738..5075)
                     /locus_tag="VF874_12900"
     CDS             complement(3738..5075)
                     /locus_tag="VF874_12900"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX9176928.1"
                     /translation="MRVARTEAFDAPTVIRTKRDGGRLSAEAIDWVVRAYTDGEVADE
                     QMSALLMAIFLRGMDRDEIACWTAAMVASGERLDFSDLRRDDKPLALVDKHSTGGVGD
                     KITLTVLPVVITCGGTVPQAAGRGLGHTGGTLDKLESIPGFTSELSKGRIAQQLCDVG
                     AAIFAAGELAPADRKIYALRDVTGTTESLPLIASSVMSKKIAEGTRALVLDCKVGSGA
                     FLKTEAESRELARTMVDLGTAHGLVTRALLTDMNTPLGATVGNSVEVTEALEVLGGGG
                     PPDVVELTLALAAEMLDAAGLDGRDPADTLRDGSAMDSFRAVVASQGGDASAPLPLGA
                     HAETVRADRTGTMGDIDAMAVAMAAWRLGAGRARPSEPVQYGAGVRIHRRPGEPVTAG
                     DPVFTLYTDTPERMSQAVAELDGGWRIAPVCSSPERLIAEFSRRPLVIDRIVP"
     gene            complement(5066..5875)
                     /locus_tag="VF874_12905"
     CDS             complement(5066..5875)
                     /locus_tag="VF874_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176929.1"
                     /translation="MTIRTSAVREIWISAVLILPAFVLYAVGPYGQAVWWVHLLSQRF
                     LAELYLVAVVAVWGRSARRRILAVVVGLVGILVERGLSATIYRFRAGEHFSSLDFFAG
                     FHYLSSRGDVEGAEALHYGGLDDVMSIFRVSYPAMILFVAAWGIARRRTPWWLLGLLV
                     SFPVIVLAWRAGYETHQLVVEGPWLGNRLFDIGVFVLCCLICWAFDAGGLALRRAGAV
                     EEAGDSRVPWLPLAGATVGVVVLCAIGYLVSAPPPQPHCKYHADSMTATCG"
     gene            complement(5872..6279)
                     /locus_tag="VF874_12910"
     CDS             complement(5872..6279)
                     /locus_tag="VF874_12910"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX9176930.1"
                     /translation="MSTDIDWEALRDKAIAVADRAYAPYSGFPVGAAALVDDGRIVAG
                     CNVENASYGLALCAECAVVCALHSSSGGRLVALVCVDGAGRLLMPCGRCRQLLMEHGG
                     AELLIDAPDGPRTLGDLLPDAFGPDDVFGRRDT"
     gene            6371..6781
                     /gene="sdhC"
                     /locus_tag="VF874_12915"
     CDS             6371..6781
                     /gene="sdhC"
                     /locus_tag="VF874_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX9176931.1"
                     /translation="MVVGGLVSSTTSPGRKTIYRGDPGMWAWVLHRISGATIFFFLFV
                     HVLDTALVRVSPQAYNEVVETYKTPVVGLMEAALVAAVLYHALNGIRVTLIDFWSQGP
                     RYQRLMLWVVGVVWVAVMVPALIVLGAHIVERFG"
     gene            6778..7167
                     /locus_tag="VF874_12920"
     CDS             6778..7167
                     /locus_tag="VF874_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HEX9176932.1"
                     /translation="MTALDSPRAPRRRGQFEKRAWLFMRFSGIVLVFLALGHLFVTLM
                     VDDGVYRIDFNYVALRWNSPFWRVWDLLLLWLAVLHGGNGMRTIIADYAHKDLTRFWL
                     NVLLAISMTFTLMLGTYVLVTFNPNIS"
     gene            7164..8918
                     /gene="sdhA"
                     /locus_tag="VF874_12925"
     CDS             7164..8918
                     /gene="sdhA"
                     /locus_tag="VF874_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX9176933.1"
                     /translation="MIQQHWCDVVIVGAGGAGMRAAVEAGPRMRTAVLTKLYPTRSHT
                     GAAQGGMCAALANVEDDNWEWHTFDTVKGGDYLADQDAVEIMCREAIDAVLDLERMGM
                     PFSRTADGRIDQRRFGGHTRDHGKAPVRRACYAADRTGHLILQTLYQNCVKHDVEFFN
                     EFYVLDLCLTETPGGPVATGVVAYELATGDIHVFHAKAIVIATGGSGRMYKTTSNAHT
                     LTGDGIGIVFRKGLPLEDMEFHQFHPTGLAGLGIVISEAVRGEGGRLLNGEGERFMEH
                     YAPTIVDLAPRDIVARSIAREVLDGRGAGPRKDYVHLDVRHLGEDVLEAKLPDVTEFA
                     RTYLGVDPVTDLVPVYPTCHYLMGGIPTTVHGEVLRDNTTTVPGLYAAGECACVSVHG
                     ANRLGTNSLLDINVFGRRAGIAAAEYAEGHDFVDLPPEPEAEVVSWVGGILSEHGTER
                     VADIRTALQQSMDDNAAVFRTEETLTQALADVRALKARYTRMSVQDKGKRYNSDLLEA
                     IELGFLLELAGVTVVGALNRKESRGGHAREDYPERDDTNCTRHTMAYKQGSELLSDIR
                     LDHKPVVKTRYEPMERKY"
BASE COUNT         1489 a         3071 c         3040 g         1424 t
ORIGIN      
        1 gcacgcgacc cattggcgcc gccgcctcgc gcaagttccc ggacgacggt tcctgcggtc
       61 gcgcgtctgg cctcgtcgcc gccacctccc gcggcgacgc accggagaaa cgcgttgaga
      121 cgacggctgg gtatcggcgc actgctggcg gtggtaggcc ccgggctgct cgccggcctc
      181 tccgacgacg acccggccgg catcaccacc tacttggtac tcggcgcgaa ttacggctac
      241 cagctgttgt gggtgctgct gctgtccacc gtcgcgctcg tgatcttcca cggcctggca
      301 gcccggatgg gcgtcgtcac cgggcagggt ctcatcggcc tggtgcggca gcggtacggc
      361 gtgcggttgg gcggtgcggt gctggccgcg ctcatcgtcg ccaacatcgg caccacgtgc
      421 gccgaattcg ccggaatcgc agcaggattc gagctgttcg ggatcacccg ctatctgagc
      481 gttccggcgg cggccgtgat cgtctccctg ctggtgctgc gcgggagttt ccaccgcgtc
      541 gagcacttcc tgctgctgct gtcgacggtg tttctggcct acatcgtgtc cggcgtgctg
      601 gcccacccgg actggggcgc tgccgtgcac ggcacgctcg tcccgaccat gacccccggc
      661 accgaatcga tcgcgatcgt caccgccacc ctcggcacga cgctcgcccc gtgggggctg
      721 tcgttcatcc agtcgtatgc ggtggacaag aaactgcgga ccgaggacct gcccgccgaa
      781 cgggtggacg tcgtcaccgg cgccgtgctg accggggtca tcgggttctt cgtggtggtg
      841 gcgtgcgccg cgacgctcta ccgagacggc cagcccatca acgacgccgc cgacgccgcc
      901 cgggccctcg agccgctcgc gggcgcggcg gcctcgcaac ttttcgctgt cggactgatc
      961 gggtcggcgt tcctggccgc ctcgatactg ccgctctcga cggcctactc ggtatgcgag
     1021 tacgccggcg tcgaagcagc gctcgacgac ccgtttcgcg aggcacgcac gttctacctg
     1081 acgttcggga tcgtcacggt gctgggcgca gtcgccgtgc tgatcccgaa tgttccgctg
     1141 gtgacggtcc tggtggccac ccaggtgctc aacgccgtcc tgctggtgcc gctgctggtc
     1201 gcgatggtcg gtatcggccg cgatcgcgac ctcatgggcc gcttccggat cggccgagcc
     1261 gggacgctgg cctacggcgt cacgacggcc gcggtggtgg cgtgcgtcgc cgcgctggcg
     1321 gtcacgtcgc tcatcgacta aggggcactg gctaggctgt ggcccgtggc tgacatcgtg
     1381 ccgatcgagc tggctttgga cgaccgcgac gtgttcactc tgtacgcgcc gcgctggcgc
     1441 gacgccagcg acgagtggga ggcgttcctc ggcaaagagg atgagctcta cgcgttcgag
     1501 tcggtgcccg acctggtcgc gtttgtccgg tcgtccgccg acaacgacct caccgaccat
     1561 ccggcgtggg aaacgctgac gaccgcgtcc gcgcacaggc tgaagccgaa gaacgacaag
     1621 cgcttcgact tggtcaacgt ctacgagctg ctcacggaaa agccgacgca ggattccgtc
     1681 gagcagctgg ccggcacgct ggcgattgcg tcggcgatcg gatcggtgtg cgaactgcct
     1741 gccatcagca agttcttcaa cggcaacccg gtgctgtcct cggtgtcggg cggaatcgcc
     1801 gacttctccg acagggccgg gctgcggcgc tggaacgaga tcggcgccgt cgtcggaaag
     1861 agctgggaca aggtcatcga cgcgatcgag gacgtcatct ccacacctga ggtcgacagc
     1921 aaggcatcgg ttaaggccga ggccgagctc gacgagccgg cgccggagga ggatgaggat
     1981 gagggcgtcg agggcggcga ggacgaaggc gagggcggcg aggccgccgt cgcggacacc
     2041 gaaaccgatg agagcgaaga gcccacgaag gctcccgccg gcgacgtcgt gctcggtgcc
     2101 gacgaagact tctggggcaa ggtgggcatc gacccgatcc ggatgatgac cagcagcggg
     2161 acgttctaca cgctgcggtg ctacctcaac gacgagccga tcttcctcgg ccgcaacggg
     2221 cggatcagcg tgttcggctc ggagcgggcg ctggcgcggt atctggccga cgagcacgac
     2281 cacgacctgt cggacctcgc gacgtacgac gacatccgca ccgccgccac cgacggctca
     2341 ctgcgcgtcg acgtcaacga cgaaaacgtg tacgtgctga cgggcatcgc cgacgacctc
     2401 gccgacggcc cggacgcgat cgaccgcgag cagctggggt tggcggtcga actgctccgc
     2461 gacgtcggcg actactccga ggacagcacc gtcgacgagg ttctggagcc cgacatgccg
     2521 ctcggcaagc tggtggctta cgtgttggac cccgacacgg cacgcaagcc cgggcccccg
     2581 tacgccaagg cggtcgagca gtggagcgag ctggacagct tcgtcgagtc gcggctgcgg
     2641 caggagtagc cgctacccga tcagcacggc gaagcgcggc ttgatcacgt cgtcgatgat
     2701 cgccagccgc tcgtcgaacg ggatgaacgc cgacttcatc gcgttgatcg tgaaccgggc
     2761 caagtcactc cacccgtagc cgaacgcctc caccagtcgc agcatctcct ggctcatcgt
     2821 ggtgtcgctc atcaaccggt tgtcggtgtt gacggtgacc cggaatcgca gccgggccag
     2881 caggtcgaac ggatgctcgg cgatgctgcg gaccgcgccg gtctgcacgt tggagctcgg
     2941 gcacatctcc agcggaatgc gtttgtcccg cacgatcccc gcgacgcgtc ccaatgtgta
     3001 agtgccgtcc gggttttcgg agatgtcatc gacgatgcgc acgccgtggc cgagccggtc
     3061 cgcaccgcag aatgcgatcg cctcgtggat ggacggcaac ccgaacgctt ccccggcgtg
     3121 aatcgtgaag cggccgttgt ggtttcgcat gtactcgaac gcatcgaggt gacgcgtggg
     3181 cggatggccc gcctcggcgc cggcgatgtc gaagccgacc acgcccttgt cgcggaaccg
     3241 aattgccagc tcggcgatct ccagcgagcg ggcggcgtgc cgcatcgcgg tgaccaaaca
     3301 gcgcaccgtg atggtccgtc cgtcggcgcg gacagccttc tcgccgtcgg cgaacccggc
     3361 cagcaccgcg tcgacgacgt cgtcgagcga cagtccctcg gctatgtgca gttccggagc
     3421 gaaccgcacc tcggcgtaga cgacgttgtc ggcggctagg tcttcgacgc actcgtaggc
     3481 cacccggtag agcgctgcgg gcgtctgcat caccccgacg gtgtgcgcga acggctcgag
     3541 gtagcgctcc agcgagccgc tgtgggccgc agtacggaac caggcggcca gctcgtcggc
     3601 gtcggtggcg ggcaggttct cgtacccgac ctgctctgcg agctccagca cggtcgccgg
     3661 gcgcaggccg ccgtccaagt ggtcgtgcag cagcgccttg ggcgcctgcc cgatcatgtc
     3721 caggttcagc ggcgtcgtca tggcacgatt ctgtcgatca caagtggccg gcgggagaac
     3781 tcggcgatga gcctctcggg cgaagagcaa acaggagcga tacgccagcc gccgtccagc
     3841 tcggcgacag cctggctcat ccgctcgggc gtgtcggtgt agagggtgaa caccgggtca
     3901 cctgccgtca ccggctcacc gggacgccgg tggatccgca cacccgcgcc gtactgtacg
     3961 ggttcgctcg gccgggcccg gcccgcgccg agccgccacg ctgccattgc caccgccatc
     4021 gcatcgatgt cacccattgt ccccgtccgg tccgcgcgca ccgtttccgc gtgtgcgccc
     4081 agcggcagcg gcgccgaggc gtcgccgccc tgcgaggcga ccaccgcccg gaagctgtcc
     4141 atcgccgagc cgtcgcggag tgtgtccgcc gggtcgcgcc cgtcaagacc cgccgcgtcg
     4201 agcatctcgg cggccagcgc gagcgtcagc tccaccacgt cgggaggtcc tccaccgccg
     4261 agcacctcca acgcctcggt gacctcgacc gagttgccga ccgtcgcgcc cagcggcgtg
     4321 ttcatgtcgg tcagcagcgc tcgtgtcacc agtccgtgcg cggtgccgag gtcgaccatc
     4381 gtgcgggcca gttcgcgaga ctcggcctcc gtcttgagaa atgcccctga cccgaccttg
     4441 cagtcgagca ccaaagcccg cgtcccttcg gcgatcttct tgctcatcac cgagctggcg
     4501 atcaacggca gcgactcggt ggtgccggtg acgtcgcgca gcgcatagat cttgcggtcc
     4561 gccggcgcga gctcaccggc cgcgaagatc gccgcgccga cgtcgcaaag ttgttgtgcg
     4621 atccggcctt tggacagttc ggaggtaaac ccggggatcg attccagctt gtcgagcgtg
     4681 ccgccggtgt ggccgagccc gcggcccgcc gcctgtggca ctgtgccgcc gcacgtgatc
     4741 acgaccggaa gcacggtgag cgtgatcttg tcgccaacgc cgccggtgga gtgcttgtcc
     4801 accaacgcca gcggcttgtc gtcgcggcgc agatcggaga agtcgagccg ctcccccgac
     4861 gccaccatcg ctgcggtcca gcacgcgatc tcgtcgcggt ccatgccgcg caggaagatc
     4921 gccatcagca gcgccgacat ctgctcgtcg gcgacctcgc cgtcggtgta cgcgcgaacc
     4981 acccagtcga tagcctcggc ggacagccgg ccgccgtcgc gcttggtcct gatcaccgtg
     5041 ggcgcgtcga aggcttcggt gcgcgctacc cgcatgtcgc ggtcatcgag tcagcgtgat
     5101 acttgcagtg cggctgtggc ggaggcgcgc tgacgagata gccgatcgcg cagagcacga
     5161 cgacgcctac ggtcgcgccg gcgagcggca gccacggcac acggctgtcg ccggcctctt
     5221 caaccgcccc ggctctgcgc agcgccaagc ccccggcgtc gaatgcccag caaatcaggc
     5281 agcacagcac gaagacgccg atgtcgaaca accgatttcc gagccacgga ccctcgacca
     5341 ccagctggtg agtttcgtag cccgcccgcc aggccagcac gatgaccggg aacgacacca
     5401 gtagccccag cagccaccac ggggtgcgcc ggcgggcgat accccacgcc gccacaaaga
     5461 gaatcatcgc cggatagctc acccggaaga tcgacatcac atcgtcgagg ccgccgtagt
     5521 gcagggcctc ggcaccctcc acatcaccac ggctcgacaa gtagtgaaaa cccgcaaaga
     5581 agtcgaggct ggagaagtgc tcgcccgcgc ggaagcgata gatggtcgcc gacaatccgc
     5641 gctcgacgag gatgccgacc agcccgacga cgaccgcgag tatccggcgc cgggccgatc
     5701 gtccccagac cgcaacgacg gcgacgaggt acagctcggc gaggaatctc tggctcagca
     5761 gatgcaccca ccacacggcc tgcccgtagg ggccgacggc atagagcacg aacgcgggaa
     5821 ggatcagcac cgcactgatc cagatctctc ggaccgccga cgttcgaatg gtcacgtgtc
     5881 gcgccgcccg aagacgtcgt cgggcccgaa cgcgtcgggc agcaagtcgc ccagcgtccg
     5941 cggaccgtcg ggcgcatcga tgagcagttc ggcgccgcca tgctccatca gcagctgtcg
     6001 acaccgcccg cacggcatca gcaatcgccc cgccccgtcg acgcagacca gggccaccag
     6061 cctgccgccg ctgctggagt gcagcgcgca caccactgcg cactcggcac agagagcaag
     6121 gccatatgag gcattctcca cattgcagcc ggccacgatg cggccgtcgt cgaccagcgc
     6181 cgcggcaccg accgggaagc cggagtacgg cgcatacgca cggtctgcga ccgcaatcgc
     6241 cttgtcccgc agggcttccc agtcgatgtc agtgctcacg gtcgccgctt ccgccgtcgt
     6301 tgtgaatccg tcccccgtca ccctaggtcg tcggagcgct gaaatgggtt tacagtccaa
     6361 aagtccaccg atggtcgttg gaggtctcgt gagcagcacg acatcgccgg gccgcaagac
     6421 gatctatcgc ggcgaccccg gcatgtgggc gtgggtgctg caccgcatct ccggcgcgac
     6481 gatcttcttc ttcctgttcg tgcacgtcct ggacaccgcc ctggtgcggg tcagcccgca
     6541 ggcctacaac gaggtcgtcg agacctacaa gacgccggtc gtcggcctga tggaggcggc
     6601 cctggtcgcc gcggtgctct accacgcgct gaacgggatc cgcgtcacgc tgatcgactt
     6661 ctggtcgcag ggaccgcgct accagcggct gatgctgtgg gtggtcggcg tcgtctgggt
     6721 ggcggtgatg gttcccgcgc tgatcgtgct gggcgcacac atagtggagc ggttcggatg
     6781 acggcgctgg acagtccacg tgcgccgcgg cggcgggggc agttcgaaaa gcgcgcctgg
     6841 cttttcatgc ggttctccgg gatcgtgctg gtgttcctgg cgctgggtca cctgttcgtg
     6901 acgctgatgg tggacgacgg cgtgtaccgc atcgatttca attatgttgc gctgcggtgg
     6961 aattcgccat tttggcgggt gtgggatctg ctgctgctgt ggctggccgt gctgcacggc
     7021 ggcaacggca tgcgcacgat catcgccgac tacgcccaca aggacttgac ccggttctgg
     7081 ctcaacgtgc tgctcgccat ctcgatgacg ttcacgctca tgctgggcac ctacgtgctg
     7141 gtgacgttca acccgaacat ctcatgattc agcagcattg gtgcgacgtg gtgatcgtcg
     7201 gcgcgggcgg ggccggcatg cgtgcggcgg tcgaggcagg cccgcgcatg cgcacggcgg
     7261 tgctgaccaa gctgtacccg acacgcagcc acaccggcgc cgcgcagggc ggcatgtgcg
     7321 cggcgctggc caacgtcgaa gacgacaact gggaatggca cacgttcgac accgtcaaag
     7381 gcggcgacta cctcgccgac caggacgcgg tcgaaatcat gtgcagagaa gccatcgacg
     7441 cggtgctcga cctcgagaga atggggatgc cgttcagccg aaccgccgat ggccgcatcg
     7501 accagcgccg cttcggcggc cacacccgcg accacggcaa ggcgccggtg cgccgggcct
     7561 gctacgcggc cgaccgcacc ggccatctga tcctgcagac gctgtaccag aactgcgtca
     7621 agcacgacgt cgagttcttc aatgagttct acgtgctgga cctgtgtttg acggaaacgc
     7681 cgggcggccc ggtggcgacg ggcgtggtgg cctacgagct cgccaccggc gacatccacg
     7741 tcttccacgc caaagcgatc gtgatcgcca cgggcgggtc gggacggatg tacaagacca
     7801 cgtcgaatgc gcacaccctc accggcgacg gcatcggcat cgtcttccgc aagggacttc
     7861 cgttggagga catggagttt caccagttcc atccgacggg cctggccggg cttggaattg
     7921 tgatctctga ggcggtgcgc ggcgagggcg gtcggctgct caacggcgag ggcgagcggt
     7981 tcatggagca ctacgcgccg acgatcgtcg acctggcgcc gcgcgacatc gtcgcccggt
     8041 caatcgcgcg cgaggtgctc gacggccgcg gcgcgggccc gcgcaaggac tacgtccacc
     8101 tcgacgtgcg ccacctcggc gaagacgtgc tcgaggcgaa gctgcccgac gtcaccgaat
     8161 tcgccaggac ctatctcggc gtggacccgg tcaccgacct cgttccggtg tacccgacgt
     8221 gtcactacct gatgggcggt atcccgacga ctgtgcacgg agaagtgctg cgggacaaca
     8281 ccacaacggt gcccgggttg tacgcagccg gcgagtgcgc gtgcgtgtcg gtgcacggcg
     8341 cgaaccggtt gggcaccaat tcgctgttag acatcaacgt gttcggccgt cgagccggca
     8401 tcgccgcggc cgagtacgcc gagggtcacg acttcgtgga tctgccgccg gagccggaag
     8461 ccgaggtggt cagctgggtc ggcggcatcc tctccgagca cggcaccgaa cgcgtcgccg
     8521 acatccgcac cgcgctgcag cagtcgatgg acgacaacgc cgccgtgttc cgcaccgagg
     8581 agacgctcac gcaggcgctg gccgacgtcc gcgcgcttaa agcgcggtac acccggatgt
     8641 cggtgcagga caaagggaaa cgctacaaca gcgatctgct ggaggcgatc gaactgggct
     8701 tcctgctgga actcgcaggg gtcacggtgg tgggtgcgtt gaaccgcaag gaatctcggg
     8761 gcggacacgc ccgcgaggac tacccggagc gtgacgacac taactgcacg cgtcacacga
     8821 tggcgtacaa gcaagggagc gaactgcttt cggacatcag gctggaccac aagccggtgg
     8881 tgaagacccg ctacgaaccg atggagcgca agtactgatg gtcgatgagc cgcttgcgcg
     8941 aagagaagaa ggcgccgcct ccgagttgcc gccggtgccc gacggcgcgg tgatggtgac
     9001 gctgaagatc gcccgcttca accc
//
LOCUS       DASWBF010000350         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1284179,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000350 DASWBF010000000
VERSION     DASWBF010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284179"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VF874_12930"
     CDS             complement(<1..1115)
                     /locus_tag="VF874_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9176934.1"
                     /translation="MGAGAHTQMSRFVEAVTLTGLRWVRLEPLTAGHVDEIASAASDG
                     DPGALWFTMAPSPETAGDWIDRMLALQAAGGLTFLVRRLDGTPIGSSSYLNVDGPNRR
                     LEIGATWYSASARRTGVNSETKLLMLGHAFDTLGCVPSSSARTSSTSPAAPPSNDSAP
                     SSTACCAAISFCPTGRGATQWCTQSSTSNGLRCETISRRAAPHRRNRIPGRDFRRFTA
                     LASLSPEEKTGGVRAIGGDRRVLVVAILASFVAFLDGSVVNLALPAMARDLHGGLALQ
                     QWIVDSYLLTLGALILVAGSISDVFGRVPVLRWGLIAFGVGSALAAAAPSGLVLVIAR
                     LIQGAGAAFLVPSSLALINSTFTQDDQPRAVGVWTAWT"
     gene            complement(1088..1519)
                     /locus_tag="VF874_12935"
     CDS             complement(1088..1519)
                     /locus_tag="VF874_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176935.1"
                     /translation="MIRLRPGWLVVLFAAVIAVSGWLPWRSTTANGGGHSSAIGGWVG
                     YFGPPSKFDAGQLIVLLASGLIVAGAMTARGLSARMSAVAALVLSLSIGGLTVVYYQQ
                     KIHPPVDVAYGLYIGAAATVVAVICSVWAMFSAWAPEHIPR"
     gene            1564..2004
                     /locus_tag="VF874_12940"
     CDS             1564..2004
                     /locus_tag="VF874_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9176936.1"
                     /translation="MTQIPDWAQRLNMSPHPEGGWFCETWRSELAIAQSALPPDYSGP
                     RSAGTAILFLLMPGQQSAWHTVRSAELWLFHRGSPLLLEVGPEQATATTHLLGADFYA
                     GQQPQILVPPGHWQRARPRDAQPSLVSCVVVPGFDYADFALAAE"
     gene            2016..>2444
                     /locus_tag="VF874_12945"
     CDS             2016..>2444
                     /locus_tag="VF874_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9176937.1"
                     /translation="MRGHTIVCGDDALATRIVEELNSAGASVVMLTSAAELAEAGIAH
                     ATAVVCVAHDDATNLEIALLAREANPNVRVVARLVNTVLRDAVAAGNGPGAILDVADL
                     AASSVVEACLSRTTHPITIPGVEVVVSGTEAPRQATLRELF"
BASE COUNT          437 a          887 c          777 g          343 t
ORIGIN      
        1 gtccaagcgg tccagacgcc gaccgctctc ggctgatcgt cctgggtgaa cgtggagttg
       61 atcagtgcca gcgagctcgg caccaggaat gcggcgccgg cgccttgaat caggcgggcg
      121 atgacgagca cgagtccgga cggcgctgcc gcggcgagcg ccgagccgac gccgaatgcg
      181 atcagccccc agcgcaacac aggcacgcgc ccgaacacgt cggagatcga gccggccacc
      241 aggatcagcg ccccgagagt caacaggtag gagtcgacga tccactgctg cagcgcaagg
      301 ccgccgtgca gatcgcgcgc catcgccggc agcgccaggt tgacgaccga accgtccagg
      361 aacgcgacga acgacgcgag gatcgcgacc accagcacgc gccggtcgcc gccgatggcc
      421 ctcactcccc cagtcttctc ctccggcgaa agtgaagcca gggctgtgaa tcgtcgaaaa
      481 tcacgacctg gaattcggtt tcggcggtga ggtgcggcgc gacgtgagat ggtttcgcac
      541 cgaaggccat tcgacgtcga ggattgagta caccactgtg tcgcgccgcg acccgtcggg
      601 cagaagctga tggctgcgca gcacgccgtc gagcttggcg ccgagtcgtt cgatggcggt
      661 gcggctggtg aagttgaaga agtgcgtgcg gaactcgacg gcacgcagcc cagggtgtcg
      721 aaggcgtggc ccagcatcag cagcttcgtc tccgagttga cgccggtgcg acgcgccgac
      781 gcggaatacc acgtcgcacc gatctcgagc cgccggttcg gcccgtcgac gttgaggtaa
      841 ctcgacgatc cgatcggcgt gccgtcgagt ctgcgaacca gaaacgtcag cccgcccgcc
      901 gcctgcagcg cgagcatccg gtcaatccag tcaccggccg tctccgggga cggcgccatc
      961 gtgaaccaca gcgcacccgg gtcgccatcg cttgccgcag aggcgatttc gtcgacatga
     1021 ccggcggtca gcggctccag ccggacccac cgcaaaccgg tgagagtcac ggcctcgacg
     1081 aaccggctca tctgggtatg tgctccggcg cccacgccga gaacattgcc cacaccgagc
     1141 agatcaccgc cacgaccgtc gcggccgcac cgatgtagag cccgtacgcg acgtccaccg
     1201 gcggatgaat cttctgctgg tagtacacga ccgtcagccc gccgatcgac aacgaaagca
     1261 ccagcgcggc aacggcggac atccgcgccg acagcccgcg cgcggtcatc gccccggcca
     1321 cgatcagacc cgatgccagc agcacgatca gctgtccggc gtcgaacttc gacggcggtc
     1381 cgaaataccc cacccagccg ccgatcgcgc tggaatgccc gccgccgttc gcggtcgtgc
     1441 tccgccacgg cagccatccg ctgaccgcga tcaccgccgc gaacagcacc accagccaac
     1501 cgggacgcag ccggatcatg gttggcacac tatcgcgccg aggcgacact actgtgaacc
     1561 ttcatgacgc agattccgga ctgggctcaa cggctcaaca tgtcacctca cccggagggc
     1621 ggctggttct gcgaaacctg gcgcagcgaa ctggcgatcg cccagtcggc gcttccgccc
     1681 gactacagcg gcccgcgcag cgccggcact gcgatcctgt tcttgttgat gcccgggcag
     1741 cagtcggcat ggcacaccgt acgcagcgcc gaactgtggc tgtttcaccg cggtagcccg
     1801 ctgctgctcg aggtcggtcc cgaacaagca actgccacaa cccatctgct gggcgccgac
     1861 ttctacgccg ggcagcagcc tcagatcctg gtgccgcccg gccactggca gcgcgcccgc
     1921 ccccgcgacg cccagccgag cctcgtcagc tgcgtggtgg tgccgggctt cgactatgcc
     1981 gacttcgcgc tggccgcgga gtaacctttc cgagaatgcg gggccatacc atcgtctgcg
     2041 gcgacgacgc cttggcgacg cgcatcgtcg aggagctcaa ctccgccggc gcctccgtcg
     2101 tgatgctgac ctcggccgcc gagctcgccg aggccggcat cgcgcacgcc accgccgtcg
     2161 tctgcgtggc ccacgacgac gcgaccaacc tcgaaatcgc gctgctggca cgtgaagcca
     2221 atccgaacgt gcgcgtggtg gcgcgcctcg tcaacaccgt gttgcgcgac gcggtcgcag
     2281 caggcaacgg ccccggagcg atcctcgacg tcgccgacct ggcggcatcg tctgtcgtcg
     2341 aagcctgcct gtcacgcacc acacacccga tcacgatccc cggcgtggag gtcgtcgtct
     2401 ccggcaccga agcaccgcgg caggcgacgc tgcgcgagct gttc
//
LOCUS       DASWBF010000351         4621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_882864,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000351 DASWBF010000000
VERSION     DASWBF010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882864"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF874_12950"
     CDS             <1..211
                     /locus_tag="VF874_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEX9176938.1"
                     /translation="NSLLIVTWDEDDNGADNHIPTVFYGAHVPVARYGGPINHYSVLS
                     TLEEFYGLPKTGSAAAAAAIPGIWV"
     gene            761..928
                     /locus_tag="VF874_12955"
     CDS             761..928
                     /locus_tag="VF874_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176939.1"
                     /translation="MTVTGGQLSLHVSDHHGDAPVVPGAGMVAGGCVPGWLRWAGPAV
                     LASAAALYLVV"
     gene            967..2277
                     /locus_tag="VF874_12960"
     CDS             967..2277
                     /locus_tag="VF874_12960"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX9176940.1"
                     /translation="MYRFGGQRIVGGRDLYSIGLFGSRRILLFTYTPFAAICSVPLTL
                     LTPMWLQTLSLASVPALLGDAVRRSLEALHTGAASRMWPLAALLIGLCLWLEPVRYSA
                     QWGQINVLLLAVVIADVLSSPAHKWAGVGVGLVAGIKLTPLIFLIYLGLIGRLRAALL
                     ASAAFAATVVVSFVVAPQDSQSYWLQRYFAEPARIAPDPAVSSSLRGLFLRLHYPAGV
                     ATAAAVLLLMAAGLAVATLACRRGQPVLGVAIVGLTAPAASPFSWSHHWVWFAVLLAH
                     LGYLGYVGGSRRAHWSMWLLWALLAGWPTGWTGKTPPFGLVTLRPGGTWNAVLPSAYV
                     LVLLIVLASTTVWLWRDLASRSWIFPRRGFQANSAVRSANNDALTATGSAVTAAARIA
                     FGHIRIGGHRAARTRFLSVRRTPRACIIRSISRISAPIAVIRRC"
     gene            2782..3636
                     /locus_tag="VF874_12965"
     CDS             2782..3636
                     /locus_tag="VF874_12965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9176941.1"
                     /translation="MPNLNRRGFLTRLSAALFLPPAVATAVEAQRYLFGAAPQAAAPT
                     AAAALAANLPAPWAASQTVLPPPPVSARIALPGPGVLSVLPGDGDLLALTVDDGVSTD
                     VVRLYTQFAKDTGIRLTYFVNGTYRSWTDNAALLRPLVDSGQIQLGNHTWSHPDLTTL
                     SAGRIGDEITRNDTFLKNTFGVDARPYLRPPYGKHNAAVRAVATDLGYTTTTMWSGSL
                     ADSTVVTEDFIRKMADTYFRPQNIVIGHLNHLPVTHVYSRLVDIIRSRQLRTVTFNDV
                     FLRPYDSK"
     gene            complement(3878..>4621)
                     /locus_tag="VF874_12970"
     CDS             complement(3878..>4621)
                     /locus_tag="VF874_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEX9176942.1"
                     /translation="NGWELRTCARRGHITYAPDDAALASRLSGTTGSGQVWRCLRCGE
                     FILGPPHGRGHPDDAPMVLRGKALRQAIIVRALAVERGFRALLIGLAAYAVWKFRGAQ
                     GSIQGALERDLPLLRASGIKVDQMATVHELEKALATKPSTLALLFFLLVGYALIELVE
                     AVGLWLLKRWGEYFAVVATAIFLPLEVHELTKGITLTRAVTFAINVAAVIYLLVSKRL
                     FGLRGGRKGYDEERRGEQLLEVEQAAARR"
BASE COUNT          731 a         1598 c         1478 g          814 t
ORIGIN      
        1 caacagcttg ctgatcgtca cgtgggacga ggacgacaac ggcgccgaca accacatccc
       61 cacggtgttc tacggcgccc acgtcccggt cgcgcgctat ggcggcccga tcaaccacta
      121 cagcgtgctg tccacactcg aagagttcta tgggctgccc aagaccggat ctgccgcggc
      181 tgccgcggcg atacccggca tctgggttta gcaacgcggt tgccctcgct gccttttcga
      241 agaagcgttc tttctgccgc tgccccggca gtttcgcgaa cgcttgagca tccccttgat
      301 cctgctcgcc ggcatcagca attacgatcg tcgagaacac ccgcaatgag cgcttcgaat
      361 tcctggcgat agggcgagag tcctattgcg agatccgaac acagtcaaca tgtttcctga
      421 cggacaagac accgaaggat ctgtattcat cgcacgaaca gcatgtcccg ctgtttacac
      481 cggcggctac tgacggcgct aggcaggccc ggcaaaggcg ctgccgatca ctgcggcact
      541 gccgaccgct caacgtcgag gtcaacggct cgaatgggac ggcaatgcgg agaagtcatc
      601 agcgcccgac ccgctgcccg atgtggcagg cggaaccgtg aatcgaccag atttttccac
      661 gtgagccgat ccccgagcgc ccggtgcgcg gcagcctgat cggccagcag aaggccggac
      721 ctgtggtgtt cgccgatgcc agggcgggta cagccgtgct gtgacggtca ccggcggcca
      781 actcagcctt catgtcagcg accaccatgg cgacgcaccg gtggtcccgg gcgccggcat
      841 ggtcgcgggc gggtgtgttc ccggctggct acgctgggcc ggcccggcgg tgttggcgtc
      901 ggccgctgcc ctctacctcg ttgtctaact gcgctggccg actacttcag ccaactcgac
      961 gccttggtgt accggttcgg gggccagcgc atcgtcggcg ggcgcgacct gtattcgatc
     1021 ggactcttcg gatcgcgccg gatcctgctg ttcacctaca ccccgttcgc cgcgatctgc
     1081 tctgtgccgc tgacgctgct gaccccgatg tggctgcaga cgttgtcgtt ggcctcggtg
     1141 ccggccctgc tcggcgacgc ggtgcgccgc agcctggaag cgctgcatac gggggccgca
     1201 tcgcggatgt ggccgttggc cgccctgctg atcgggctgt gcctgtggct tgagccggtg
     1261 cggtattcgg cccagtgggg acagatcaac gtgctgctgc tggccgtggt gatcgccgac
     1321 gtgctgagct caccggcgca caagtgggcg ggggtcggcg tcggcctggt tgcgggcatc
     1381 aagctcacgc cgctaatttt cctgatctat ctcgggctga tcgggcggct tcgtgccgcg
     1441 ctgctggcca gcgccgcgtt cgcggccacg gttgtggtga gctttgtcgt cgctccgcaa
     1501 gactctcaat cctattggtt gcagcgttat ttcgcggaac cggcgcgaat cgctccggat
     1561 ccggcggtca gcagcagcct gcgcgggctg ttcctgcggt tgcactaccc cgccggggtt
     1621 gccacggcgg cggcggtgct gctgctgatg gcagccgggc tggcggttgc gacactggcg
     1681 tgccgtcgcg gccagccggt gctcggcgtc gcgatcgtgg gcttgaccgc cccggcggca
     1741 tcaccgttca gctggagcca ccactgggtg tggttcgccg tgctgctggc gcacctcggc
     1801 tacctcggct acgtcggcgg ttcacgtcgc gcgcattggt cgatgtggct actgtgggcg
     1861 ctgttggccg ggtggccgac cggctggacc ggtaagacgc cgccgttcgg gctggtgaca
     1921 ctgcggcccg gcggcacttg gaacgccgta ttgcccagtg cctacgtgct cgttctcctg
     1981 attgtgctgg cgagcacgac ggtgtggctg tggcgcgacc tggcgtcccg ctcgtggatc
     2041 ttcccgcgcc gcggctttca ggcgaattcg gctgtccggt cggcgaataa cgacgccctg
     2101 acggcaaccg gctcagccgt cacggcggcg gcgcgaatcg cattcggcca catacggatc
     2161 ggaggtcacc gcgccgcacg cacgcgcttt ctgtcggtga ggcgaacgcc tagggcgtgc
     2221 atcatccggt cgatttcgcg gatttcagcg ccgattgctg tgattcgtcg gtgctgacgc
     2281 agcaccccct cggcgtcgtg cacccgttcg tagtgggcca gcttcctcgc ctgctcgtcg
     2341 agttggtcac atacttcggc ccgcagttgg gctaacgacg agtagaaaac ctcggcctgg
     2401 cggatctggg cactgaattc gaccggcgac gcaacgatcc gttccccgac tgtcaccgcc
     2461 gtaaaataaa cggcgagccc aatacccgcg aggcgaacag ccaaacgaag cacatcacac
     2521 acggcacact ccgtcagaag tcggcaccga agcgtctcat ctcgggtgca gatgactgcg
     2581 ccgcaacgcc tttcgcgccc tgcagaagtc acctcacagc gccggctcca cgagccgcga
     2641 accaggccag caggaagctc acagccggac cgtaggctta ctctgctact gtctgccgcc
     2701 gatgaacacc ccgctacccg gcgccgccac gccgcgcaag accaggccgt cgcgcgtctg
     2761 gcccggcgag cccgcacggc gatgccgaac ctgaaccgca gaggattcct cactcgttta
     2821 agtgccgcgc tcttcttgcc ccccgcggtg gccaccgccg tcgaagccca gcgctacctg
     2881 ttcggcgccg ccccgcaggc ggccgccccc accgcggcgg ccgccctagc agccaacctc
     2941 cctgcgccgt gggccgcatc acagactgtg ttgcctccgc cgccggtcag cgcccggatt
     3001 gcgctgcccg ggccgggtgt gctttcggtg ctgcccgggg acggcgacct gctggccctg
     3061 accgtcgacg acggcgtgag caccgacgtg gtgcgcctct acacccagtt cgccaaggac
     3121 accggcatcc ggttgaccta cttcgtcaac ggcacctacc ggtcgtggac cgacaacgcg
     3181 gcgctgctgc ggcccctcgt ggattccggg cagatccagc tgggcaacca cacctggtca
     3241 caccccgatc tgaccacgct cagcgctggg cggatcggcg acgaaatcac ccgcaatgac
     3301 acgtttttga agaacacgtt cggcgtcgac gcgcggccat acctgcggcc gccgtacggc
     3361 aaacacaacg cggcggtgcg ggcggtagcc accgacctgg gctacacgac caccacgatg
     3421 tggtcgggct cgctggccga ctccaccgtg gtcaccgagg acttcatcag gaagatggcc
     3481 gacacctact tccgcccgca aaacatcgtc atcggacacc tcaaccacct gccggtcacc
     3541 cacgtctaca gccggctggt cgacatcatc cgaagccgcc agctgcgcac cgtgaccttc
     3601 aacgacgtgt tcctccgccc ctacgacagc aagtagccct ccgcgcggcg cggcggcggg
     3661 cggtacctaa ctaatcgtca gttctgggca ccggccaacc aggctgggcc gaatcgtctt
     3721 ggccaccgcc ggcgctgatg cgctgcgctg gcccatttcg cggctcggct gctgtgacgc
     3781 gcgccgctga cggggaccgg tggacttcga gacgctttgg cttgaccgcg gcggtcaccg
     3841 aaccgttcag tgcggccgag tcgacagacg cgacccctta ccgcctggcg gcggcttgtt
     3901 cgacctcgag gagttgctca ccgcggcgtt cttcgtcata gcctttgcgg ccgccccgca
     3961 gaccgaacag tcgtttcgag acgaggaggt agatcaccgc ggccacgttg atagcgaatg
     4021 tcactgcccg ggtgagggtg atgcccttgg tcagttcgtg cacctccaac ggcaagaaga
     4081 tcgcggtggc taccaccgcg aagtactcac cccagcgttt gagcagccac agtccgacgg
     4141 cttctacgag ctcgatcaac gcgtagccga ccagtaggaa gaacagcagt gccagggtcg
     4201 acggtttggt ggccaacgcc ttctccagct catgcacggt ggccatctgg tcgaccttga
     4261 ttccgctggc gcgcagcagc ggcaggtcgc gttccagggc gccttggatc gatccctgcg
     4321 caccgcggaa cttccacacc gcataggcgg caagcccaat cagcagtgcg cggaatccac
     4381 gttccaccgc cagcgcccgt acgatgatgg cctgacgtaa agccttgccg cgcagcacca
     4441 tcggcgcgtc gtcggggtgg ccgcgcccgt ggggaggccc gaggatgaat tcaccacagc
     4501 gcaagcagcg ccacacttgt ccggaaccgg tggtgccgct gagccggctg gcgagcgcgg
     4561 cgtcgtcggg tgcgtaggtg atgtggcctc ggcgcgcgca ggtccgcaac tcccacccgt
     4621 t
//
LOCUS       DASWBF010000352         6945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_883046,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000352 DASWBF010000000
VERSION     DASWBF010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883046"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF874_12975"
     CDS             <1..379
                     /locus_tag="VF874_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEX9176943.1"
                     /translation="DQMTAVHELEKALAAKPSTLAVVVLVLAAYAVLELVEGVGLWLL
                     TRWGEYFAVVATSVFLPLEVYDLVTKGMTFTRAGAFAINVAAVIYLLISKRLFGLRGG
                     REAYDEERRGEQLLDVERAAMTT"
     gene            complement(1158..1378)
                     /locus_tag="VF874_12980"
                     /pseudo
     CDS             complement(1158..1378)
                     /locus_tag="VF874_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1547..3193)
                     /locus_tag="VF874_12985"
     CDS             complement(1547..3193)
                     /locus_tag="VF874_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX9176944.1"
                     /translation="MTQPGHARSKEARAPSRGPRHRLRRRSAGLPVRTGAKAITAVFS
                     AATLILTGMGWIGYRHISAGITTSAALADEPSRSGPDQNILIMGVDSRRDQHGRPLPK
                     DIYDALHAGDANSGEYDADALIVLHVPGGHGPVTAVSVPRDDYVDLAGCPASQCRGKV
                     KHAYSLAYRRVMDEAGADTADSAATHDTGQEQKAREAGRVAEIRTVERLLQIPIDHFI
                     EVSLVALFDIAQVVEPVTVCLNDDTADPYYSGAEFHKGIQQLSAAQAVAFVRQRRDAN
                     DASFTDLDRSRRQQAFIASVIDALRRSGAASNLSMLARLGDVAEHNVAVDAGFDLAGF
                     LRGVAASSDSPVTLYTLPITEFSQTPDGQDVNIVDAASLREVIHNLLGTGLSGPAPTP
                     ELPPTAGARIMMHASASQVVFDVINASGQDGLATSLEKAFGTGQFAEGAASTAESAIA
                     ISVIEYGPGAQAAAAALADELHLTAKASNTVAPHTVQLTAGTDLAASDYLGRDATASE
                     PPSTASTSEMTPVAVSATGSQAPAPTDLTQMRADGVPCVK"
     gene            complement(3616..3888)
                     /locus_tag="VF874_12990"
     CDS             complement(3616..3888)
                     /locus_tag="VF874_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176945.1"
                     /translation="MTEKSTRNVSHRSAVDAVRDAETFVVTLPVVGRVRIPRPEQLAY
                     YSALAGLAALEIIDWPVALAIGAGHALASNHHSKVAQELGEALEEV"
     gene            3952..4107
                     /locus_tag="VF874_12995"
     CDS             3952..4107
                     /locus_tag="VF874_12995"
                     /inference="COORDINATES: protein motif:HMM:NF040653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv1535 domain-containing protein"
                     /protein_id="HEX9176946.1"
                     /translation="MSKRSGGVSADPLTDVAARLLAVPLCELYAVLWRAGVVEIVDRS
                     TADASRR"
     gene            complement(4110..6945)
                     /locus_tag="VF874_13000"
                     /pseudo
     CDS             complement(4110..6945)
                     /locus_tag="VF874_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014389928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
BASE COUNT         1070 a         2323 c         2342 g         1210 t
ORIGIN      
        1 cgaccagatg accgctgtgc atgagctgga gaaagcgttg gccgccaaac cgtccaccct
       61 cgcggtggtg gtcttggtgc tggcggccta cgcggtgctg gagctcgtgg aaggcgtcgg
      121 attgtggttg ctgacgcgct ggggcgagta cttcgcggtg gtggccacgt cggtgttcct
      181 gccgttggag gtgtacgacc tggtcacgaa agggatgacg ttcacccgcg ccggggcatt
      241 cgccataaac gtggccgccg tgatctacct gctgatctcc aagcggctgt tcggccttcg
      301 cggcggccgc gaagcctacg acgaggaacg gcgcggcgag caactcctcg acgtcgaacg
      361 agccgccatg accacctgag ccggtactgc ggcgcagatc cgatcggtgt tcccggtagg
      421 tgcccgccct gatcaaccgc cacccctggt ggccgatccc cgcgccgtcg agtcgcttgc
      481 ccggccggcg cgtcgcacag cgcccgccct ccaagagcgc gtgacgccgc agtcctctgg
      541 cgtgggcggc caccaggagc ggggaaagct tctcggggct atatgagccc ttccggggcg
      601 agcgcttcgc gacagacgag ggaagtgttg cttacggccc ttccgaggcg ttacttcgga
      661 cccttttgtt cgcccgaatt cagagtgcgt ccgcgtcggc gtcgcgagag aagcgctatg
      721 cgaccattac gccgctggcc gtgcggaccc ccccatctac gacaggtcgg cttgagcttg
      781 ccccgccagc gccggcgact ggacgacgac ccgttgatga cgccgacgtg ctggtgtatc
      841 ggcacgaagt ttcaggggag cttcgcccgc ccgcgacggc acgatggtct acacgctcgc
      901 gcgggaaccg ataagcgggt ggtcaactgc tgatatgtca gccgacgagc ccggcgggct
      961 gccgttcggg ccgatgcccg cgacctatcg gcttggcgtg atctcagcga cggtttgaag
     1021 gattcgcttg caccagcgtc agcctctccc acagttcgcc aatctcgccg tgcgccagcg
     1081 gttcagcggt gagcggtagc cgctggtcgc cagcttggcc gcgatgtttg gggacgttct
     1141 tcggcgcccc cgcggagtca gccggcgttc tccagggcca ccaaggccgc cgcgacagcg
     1201 aagaacttgt cgtgccgagc tggcggaccg tcttgatgtt gaaggcctgg gccagcgctg
     1261 cggcgtccgc atcggtcact ccggccagtg ccgccgggga ggcgtcgaga atgtctttca
     1321 gcgatttgtt ctcgtagtct ttatccagcg ccttggcgag gtcaactgag atcggcatcg
     1381 gtcctccact cacttgctgc ttacgtcaac gcctaacgct tgatcaccct aatcgggagc
     1441 gtcagagcag acctcgattc gcgacgactc gaaattgtcc gccgcgtccc cgtcggctgt
     1501 tgggccgtcg gcttagcacg ttgcgccgat cggtgtttgc atatggttac ttgacacacg
     1561 gcacgccatc ggcgcgcatc tgtgttaagt cggtcggtgc cggggcttgg gaaccggtcg
     1621 cgctgacggc taccggcgtc atctctgagg tggaagccgt ggatggcggt tctgacgccg
     1681 tcgcgtcacg gcctaggtag tcggaggctg ccagatcggt cccggcggtg agctgcacag
     1741 tatggggcgc tacggtgtta gaggccttgg ccgtcaagtg aagttcatcg gcgagtgcag
     1801 cggcggccgc ctgggctccg gggccgtact cgatcacgct gattgctatg gccgattcgg
     1861 cggtgctggc cgccccctcg gcgaattgcc ctgtgccgaa ggctttctcg agagacgtgg
     1921 cgagaccatc ctggcctgag gcattgatta cgtcgaatac aacctgagat gcgctggcat
     1981 gcatcatgat tcgggcgccg gcagtcgggg ggagctcggg tgtcggggcg ggtcccgaca
     2041 aaccggtccc cagcaggttg tgaatgacct cccgcaggga ggctgcgtcg acgatgttga
     2101 cgtcttgccc gtcgggggtc tgactgaatt cggtgatcgg caatgtgtac agggtgaccg
     2161 ggctgtcgct tgatgcggcg accccacgca ggaaaccggc caggtcgaag ccggcgtcca
     2221 cggcgacgtt gtgctcagcg acatcgccca gtctggcgag catgctcaga ttcgacgctg
     2281 cgccgctgcg acgcagcgcg tcgataaccg aggcgatgaa ggcctgctgg cggcggctgc
     2341 ggtccaggtc ggtgaacgac gcgtcgttgg catcgcgtcg ttgccgcacg aacgcgaccg
     2401 cttgcgcggc gctgagttgc tgaatgcctt tgtggaactc ggcgccggag tagtacgggt
     2461 ctgcggtgtc gtcgttcagg cacaccgtga ccggttcgac gacttgggcg atgtcgaaca
     2521 acgccaccag gctgacttcg atgaagtgat cgatcgggat ctgcagcagc cgctcgaccg
     2581 tccggatttc ggcgacccgg cctgcctccc gcgccttttg ttcctggccc gtgtcgtgtg
     2641 tggcagccga atccgcggtg tcggcgcccg cctcatccat cacacgccgg taagccaggc
     2701 tgtaggcatg tttgaccttg ccccggcatt gcgaggccgg gcagccggcc agatcgacgt
     2761 agtcgtcacg ggggacggag accgcggtca ccggtccgtg cccgccgggc acgtgcagca
     2821 caatgagcgc gtcagcgtca tattcgcctg aattcgcgtc gccggcgtgc agcgcgtcgt
     2881 agatgtcctt aggcagcggc ctaccgtgct ggtctcggcg gctgtccacc cccatgatga
     2941 ggatgttctg atccggtccg ctgcggctgg gttcgtcggc caacgccgca gacgtggtga
     3001 tgccggctga gatatgccgg tagccgatcc agcccatgcc ggtcaggatc aacgtcgccg
     3061 ccgagaacac cgctgtgatc gccttggcgc cggtgcgtac cggaagccca gccgagcggc
     3121 gacgcagcct gtgccgcggc ccgcgcgatg gtgcacgggc ttccttactg cgggcgtgtc
     3181 caggctgtgt cataggagac cgaagtatcc ccggtcgttg tgctttcgat caacccgtcg
     3241 tctgctgtgt gccagctgag gtcacatacc cgacagctgt gatggtgatg gcgactggtc
     3301 accctcgcca gatcgtggcg tgcatgacct cgagaacttc accgactaac cgcactgcgc
     3361 gtggaaattc gctgtcggtc aacactttga acaccggaac ggccgcgatg aacaggggct
     3421 agcggcggct cgacgaggcc gagcccgacc cctgcggcga gaccgccgaa atagcccacc
     3481 gaacgcggaa cgtccccttc gactcggccg acttcgacct cgatgacgtt ccccggtgcc
     3541 cgatcctgat ctgtaccgcg ctgtcggatg ccattaccct ctccgtcccg caagattcgg
     3601 ccgccgtcgc gttactcaca cctcttcgag cgcttcgccg agttcctgtg cgaccttgct
     3661 gtggtgattg ctggccaacg cgtggccggc cccgatggcc agcgcgacgg gccagtcgat
     3721 gatttcgagg gcggccagcc cggccagcgc gctgtagtac gcgagttgtt cgggtcgcgg
     3781 gatccggaca cggccgacga ccggcagcgt gacaacgaac gtctcggcgt cgcggactgc
     3841 gtccacggcg ctgcggtgag aaacattacg agtcgacttt tcggtcattg tttgcctttc
     3901 gtcatggcca attcaacggt tgggggctag gtctcggtca aagtgggcac catgtcgaaa
     3961 cggtctggcg gtgtgagtgc tgatccgttg accgacgtgg cggcgcggct gctggccgtc
     4021 ccgttgtgtg agttgtacgc ggtgttgtgg cgcgcagggg tggtcgagat cgtcgatcgg
     4081 tcaacggccg atgcgtctcg ccgctgagcg agaaaccggg atatcgcggc ggccgcgatg
     4141 gtcgccgcag tcgccgatcc gagagcctga gcccagccta cgggccccag tggtgtgctg
     4201 cccagcagct ggctgacacc cgggatgctg atgagagcac ccatcgcggc cagtgatccg
     4261 atcgcggtcg ccacgacgag cgggctgtgc gagtccagca gcgtctgacc gagctgagtg
     4321 gcgacgagcg caacgaggcc cacggtagag gcgcgttgtc gccgtccggt gacgctggcc
     4381 agtgtccatg ccagcgccgc gcccgctgcg gtcgtggctc cccggactgc gaccgtccgc
     4441 cacagcgtgg tctcgtcggg ccctcgcctc gtcggctgcc cgttggcggt gggttcgctg
     4501 accgccaacg cggccgccgg cagcgcgtcc gtcagcatgt tgaccagcag cagctggcgg
     4561 gtgttcagcg gcgaacgtcc ggtgatagcg cttccgatga tcgcgaatgc cacctccccg
     4621 gcgttcccgc cgagcagcac gcacaccgcg gattggaccc gcagccacaa cttgcggcct
     4681 tcctcgagcg cgtccagcaa cgagatgatg cggccgtcga gcagcatcac gtcggcggct
     4741 gtgcgcgctg gatcgctgcc gcgcgatgcg actccgattc cgaccgtcgc ggcgcggatg
     4801 gcggccgcat cgttggcccc gtcgccgacc atcgcgcaga cccggccggt ccgctcgatg
     4861 gtttgcacca cctgaacctt gttctccgga gacatccgcg cgaacacacg tcgatccgcc
     4921 acggcctgtt cctgcccgcg acgcgacaac gcctcccatt cggttccggt gatcacgacc
     4981 tcggcggcga ccggcatccc gagttcggag gcgatcgctg cggcggtgat cggatgatcc
     5041 ccggtgatca gtcggacacc gatgtcctga gccgccagag acgctaacag cccggccgct
     5101 tcagcgcgtg gtgtatcgga cagcccgacc aacccaatga accgcagccc tgatcggcac
     5161 agttcttcga actcgttggg gtcgtcacac gctgtttgag tctggtctgg ggtcagcggg
     5221 cgttgcgcga tcgcgatcac acgcaggcct tcgccggcca tgtcccgcac cgcccgccat
     5281 gtagcgggac cgatgtccgc gcacgccccg agaaccgcct ccggagcgcc cttcaccgtc
     5341 agctgttcgt ggttcagtgt tgcggagaac ggccgcccgg accggaagga caagtgcgcc
     5401 aacaggtctg cggacgtacg gtccgatgcg tgagcagctt cgatgattgc cgcatcggtg
     5461 gcgtgcgcgt gctgaccgtc gccatccggt gacggagcgg ccaatgcggc agaagtgagc
     5521 acctcgtcgc gcgaatgtcc gctcggggcg tacaccttgg tcacgcgcaa tcggttctcg
     5581 ctgagagttc cggtcttgtc gaagcacacc acgtcgacgc ggcccagcgc ctcgacagat
     5641 cgcggttcgc gaaccaacgc gcccagccgg gtcagccgcc gcgcagacgc ctgctgggcg
     5701 agcgtcgcta ccagcgcgag tccctccggg actgccgcca ccgtgaccgc gacgccgctt
     5761 gcgatggcct gacgcagccc ggtgcggcgc aatagcccca gccctgtgac aagcccaccc
     5821 ccggccaggc tgacaggcca tgcgcggtcg gttagctcgc gcaattgggc ctgcagaccg
     5881 actgggccgc gctccgcgcg cggcagctca gcggcgcgcc ggacctgcgt ctgcgggccg
     5941 acggcggtca cgacggcaat cccggtgccc gcgacgacgg tcgtgcctgc gaacaccatg
     6001 cagcttcgct cagccaatgc ggccccgggc gtcgcctcca cttgtttctc cacgggaaag
     6061 ctctcgcccg tgagagtgga ttcgtcgact tccagagcgg ccacctcgat taggcgcgcg
     6121 tcggcgggca ctacttcgcc cgaccgcacc tcgatgacag cacccgggag cagtcgggcg
     6181 gcttcggtgt cggcatatgc gcgcacccct cgcgcgtccg tcacgacctt gcgcgccggg
     6241 ggaacctgta ctgccaacag acgccgcagc agccgctgag cgcgcacctg ctgggtggcc
     6301 gcgagcgcgg agttgaatgt gagtaccgag ccgacgagca ccgcatcgac gggtgagccc
     6361 agcacggcgc tggccgccga gccagtggcc aagaccgggg tcaacgggtc ggagagctcg
     6421 tcgcgcagcg tggtcgcgaa ttcccacatc gtccgtcgca ctgagtcgac cattcctgcg
     6481 gatgttcgcg ccgtcgtcga caagcgcgat cttgggggca gcttcccgcc gtcgtcgacc
     6541 cgttccggca gcaacgcctg gacctgctcg accgacatcg cgtgccagtc gtccgtggcg
     6601 gccggcgcgg gcgcggatgc acgaatcacg ttgcgcgcca gtgagtatcc cgcccacagc
     6661 cccgcggctg cgccggcagt gaccggcccg ggtccgcggc cgcgcacgcc aggcaccatc
     6721 agcaacgcgc cgagcaagga cgcggccgtc gctatcttca cccctcgacg gctcgccgca
     6781 cgggcggacg gcagcgcatg caatagacgc cacgcggcag cgaggtcgtc gacaatcagg
     6841 tgcgcatacc acggcggctc ggctccaggg gccagcacgc cgaccgcgac atcggccgcc
     6901 gaaagggctt gcgccgccga gtttgacagc accaggacgg tccgg
//
LOCUS       DASWBF010000353        15682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_505389,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000353 DASWBF010000000
VERSION     DASWBF010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15682
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505389"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VF874_13005"
     CDS             complement(<1..978)
                     /locus_tag="VF874_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9176947.1"
                     /translation="MTEAVPPIGTQFSRLAAVAPDEPAVTCDGRTLTRGELDSSTNRL
                     ARAYADRGVGVGDYVTIALPNSIEWIQAALATWKLGAVPQPLSPRLPEPEFDALMQLR
                     SRALIVGRISSELPSLPMEFTPDPGLSDGPLPEAVSPTWKALAPGGSTGRPKLIEAGG
                     DSRFAATIFGQMMGAEEGDTHLVSVPLSHNTGMTAATISLLMRQHLVLMPRFDAADFL
                     RLVTDHRVNFLVTVPTIMQRVHAAYRANPDAYDLSSIRRFWHVGAPCPQAVKQAWIDL
                     LGPEVLWELYGGTELQAMTFVSGEQWLAHRGSVGVVVAGEMKVLDDDGNE"
     gene            complement(980..1957)
                     /locus_tag="VF874_13010"
                     /pseudo
     CDS             complement(980..1957)
                     /locus_tag="VF874_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(1970..6235)
                     /locus_tag="VF874_13015"
     CDS             complement(1970..6235)
                     /locus_tag="VF874_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase"
                     /protein_id="HEX9176948.1"
                     /translation="MSRRWLWAVGSAALILCLAQSPGRVSPDTKLDLTASPLRFLARA
                     TNLWSSELPFGQAQNQAYGYLFPHGAFFLLGDTLGLPGWLTQRLWWALLLTVGFWGLL
                     RVAEALGIGTTSSRVIAAAAFALSPRVLTTLGSISSETLPMMLAPWVLLPVILALRGS
                     GNVRVLAARASVAVALMGAVNAVATLTGCLAAVIWWTCHRPNRLWWRFTGWWLLGILL
                     ATTWWLVALALLGQVSPPFLDFIESSGVTTQWTSLTEVLRGTDSWTPYVAPTATAGQS
                     LVTSSVVILATTLVAAGGLAGLALSSMPARGRLVTVLLVGVVLLAVGYSGGLGSPVAH
                     EVQAFLDAAGAPLRNVHKLEPVIRLPLVLGLAHLLGRVPLPGAVPRRVWVHALAHPEH
                     DKRVAVAIVVLVALAVSTSLAWTGRLTPPGTFSAIPQYWHDAADWLDEHNDSGPTPGR
                     VLVVPGSPFATQVWGTSHDEPLQVLGESPWGVRDSVPLTPAATIRALDSVQRLLAAGR
                     PSAGLMDTLAQQGISYVAVRNDLDPDTSRSARPILVHRAIDGSPGLTKVAQFGDPVGA
                     GTVPGFVADSDLRPKYAAVEIYRVETGAAGPATFNPGAAGPYLADIDAMPRVDGGPEA
                     LLRLNERRRLQGEPPLGPALLTADAQRAALPAPVVTVTDTPLARETDYGRVDDHSSAI
                     RAPGDERHTFNRVPDYPPDAQPVHGDWVGGRLTASSSAADSTALPNVSPATSPAAAID
                     GDTATSWVSNALQAAVGQWLRVDFDNPITNATITITPSATAVGAQVRKLEIATATGTT
                     TVRFDEPGKPLTAALPYGETPWVRVTAVGTDDGSAGVQFGIIDIAITQYDANGFAHPV
                     TLRHTVAVPAPPAGSAVALWDLGSELLGRYGCADGPDRVRCAATMALAPEEPVNLSRT
                     LTVPEPVTVTPTVWVRARQGPKLADVVAQPGPARASGDADVIDIQGSAFAAADGDPRT
                     SWIAPQNVVHDRTPPTLTLKLPRPTEVAGLQLIPSASSVPTHPTLVAVDLGDGPQVRP
                     LSGSRDWGHSPVNGRYPHPLAGDDGETRTLTLTPRITDTVTISVLDWDDVIDRTALGF
                     DQLKPPGIAEVTALGADGKPIAPADADRNQARPIDLGCGRGPTIAIAGRFVQTSVHTT
                     VSALLAGEPIEAQPCQREPIALSAGEQELLISPGEGFVVDGAQLTTPLPPQAAAVAIP
                     VQVGTWGPDRREVRAEPAPTARVLVVPESVNPGWVAHTADGTRLTPVVVNGWQQGWVL
                     PAGTSGVVTLTFPSNTLYRAGLFWGLALLPLLALLALVRPRREVDDEPARPWRLPTAV
                     ATIAVLGAGWLISAIAGVVVFAGALAVRYWLRDRPSLSAAITLGTTATGFILAGAVLS
                     RHPWRSVGGYVGHSWGVQLLALVAVGALAASVVPITRRQPPVK"
     gene            complement(6273..6428)
                     /locus_tag="VF874_13020"
     CDS             complement(6273..6428)
                     /locus_tag="VF874_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2613 domain-containing protein"
                     /protein_id="HEX9176949.1"
                     /translation="MAGASSLVLGLLLGAAAVFGVTLMVQQDKKPPLSIGDGASILNR
                     VEYGNRS"
     gene            6656..7897
                     /locus_tag="VF874_13025"
     CDS             6656..7897
                     /locus_tag="VF874_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9176950.1"
                     /translation="MPSISGRTARHGQHRSLRRGLPILQVAFTVALVAAITFLGILWF
                     RHRETTNANAAPPAATSARQASNQPLTMPIGPPAAPVCSDDALTSMPIRDKLAQLLMV
                     GVTGADDAQAVVANYHVGGIIIGSWTDLSMLGSLGEITSAGTLPVAVGVDEEGGRVSR
                     LSSLIGVAPSARALAASSTPDEVYAQALARGQAMRGLGITVDFAPVVDVSDQPDDTVI
                     GDRSFSDDPATVTEYAGAYARGLRDAGLLPVFKHFPGHGHGSGDSHTTSVVTPPLSQL
                     VTNDLVPYKTLLNEQPVAVMVGHMHVPDLTGGQPASLSGAAYTLLRTGSYGGPPFGGP
                     VFTDDLSSMRAISDRYGVAQAVLLALQAGADIALWITTAEVPAVLDRLEQAVNAGELT
                     SQAVDEKVLRATGFKGRNPQC"
     gene            7951..8589
                     /locus_tag="VF874_13030"
     CDS             7951..8589
                     /locus_tag="VF874_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9176951.1"
                     /translation="MAGGTKRLPRAVREQQMLDAAVEMFSRNGYHETSMDVIAAEAEI
                     SKPMLYLYYGSKEELFGACLNRELSRFIEAVRADIDLKQSPKELLRSTIRAFMHYIDA
                     NRASWIVLYQQATSSQMYSHTVLEGRERIIDLVARLLSAGTRNPEPDTDFHMMAVALV
                     GAGEAIATRVSTGEADVNEAAELLSNLTWRGLRGRPVDEDVTPAATARSRSR"
     gene            complement(8564..9421)
                     /locus_tag="VF874_13035"
     CDS             complement(8564..9421)
                     /locus_tag="VF874_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9176952.1"
                     /translation="MSTVETTDRPSALRNLLRAAAGALPFIPRSDDVPDRTLKVEGLQ
                     IDRSNVAQYSAVTGLRYADQVPLTYPFALTFPVVMSLVTGFDFPFAAMGSVHTENHIT
                     QHRPISVTDTVNVTVHAENLREHRRGLLVDVLTDVSVGNDLAWHQVTTFLHQQRTSLS
                     DEPKPPPKKQPKLPPPNGVLSVSARQIRRYASVGGDHNPIHTNPVAAKLFGFPTVIAH
                     GMFTAAAVLRTIEGQLPDAVRYSVRFGKPVVLPAEANLYVSRVADGWDLALRNSKGEP
                     HLTGSVNAL"
     gene            complement(9493..10845)
                     /locus_tag="VF874_13040"
     CDS             complement(9493..10845)
                     /locus_tag="VF874_13040"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX9176953.1"
                     /translation="MASDLFTQVVNSTPGSFLAKQLGIPQPEPLRRYKPGEPPIAGSL
                     LIGGEGRVVEPLRSALAEDYELVRNNIGGRWADSFGGLVFDATGITEPQGLNALYEFF
                     PPLLRNLGHSARVVVVGTTPDAARSVDEHIAQRALEGFTRSLAKELRHGATMQLVYVS
                     PNAKPAATGLESTMRFLLSGKSAYVDGQVFYVSEADSTPPADWDKPLDGKVAIVTGAA
                     RGIGATIAEVFARDGARVIAVDVEASQEALGETATKVGGTALTLDVTADDAVDRITEH
                     LREHYDGRADALVNNAGITRDKLLANMDDARWSAVLAVNLLAPQRLTQGLVDNGSIGE
                     GGRVVGLSSMAGIAGNRGQTNYATTKAGMIGITQALAPVLYDKGITINAVAPGFIETA
                     MTDAIPFAIREVARRLNSLYQGGKPVDVAETIAYFASPASNAVTGNVIRVCGQAMLGA
                     "
     gene            10919..12247
                     /locus_tag="VF874_13045"
     CDS             10919..12247
                     /locus_tag="VF874_13045"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014943078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9176954.1"
                     /translation="MAGETVEANVATKKASRRVAVLGGNRIPFARSDGAYADASNQDM
                     FTAALGGLVDRFNLEGERLGAVIGGAVLKHSRDFNLMRECVLGSALSSYTPAFDLQQA
                     CGTGLQATIAAADGVGAGRYEVAAAGGVDTTSDAPIAIGDNLRRTLLGLRRAKSNVDR
                     LKLVGKLPATLGVEIPVNSEPRTGLSMGEHQAITAKQMGIARVAQDELAAASHRNMSA
                     AYDRGFFDDLVTPFLGVYRDNNLRPDASVEKLAKLKPVFGVKAGDATMTAGNSTPLTD
                     GASVALLASEDWAEQHSHEPLAYFVDAETAGVDYVNGPDGLLMAPTYAVPRLLARNGF
                     SLGDFDFYEIHEAFASVVLCHLQAWESEEYCKERLGLDAALGSIDRSKLNVNGSSVAA
                     GHPFAATGGRIVAQLAKQLAEKKKDTGGSVRGLISICAAGGQGVAAILEA"
     gene            complement(12649..14484)
                     /locus_tag="VF874_13050"
     CDS             complement(12649..14484)
                     /locus_tag="VF874_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9176955.1"
                     /translation="MSHYKSNVRDQEFNLFDVFDIDKALGQGEYSDLDGDTARDMLAE
                     MARLAEGPIADSFTESDRNPPTFDPKTHSVTLPESFKKSVRALVDGHWDKVGIDEALG
                     GMPMPKALVWALHEHILGANPAVWMYGGGAGFAQIVYHLGTEEQKKWAVLAAERGWGG
                     TMVLTEPDAGSDVGAGRTKAVKQDDGSWHIDGVKRFITSGDSGDLFENIFHLVLARPE
                     GAGPGTKGLSLFFVPKFLFDPQTGEIGERNGIYVTNVEHKMGLKASATCELSLGQHGV
                     PAKGWLVGEVHNGIAQMFDVIEQARMMVGTKAIATLSTGYLNALEYAKSRVQGADMTQ
                     MTDKTAPRVTITHHPDVRRSLMTQKAYAEGLRALYIFTATFQDSEVAKAVHGIDPKLA
                     VQVNDLLLPVVKGVGSEQAYAKLTESLQTFGGSGFLQDYPIEQYIRDAKIDSLYEGTT
                     AIQAQDFFFRKIVRDKGVALAHVAGQIEQFVKNEYGNGRLKAERALLETALQDVQAMA
                     ATLTGYLMAAQEDSTSLYKVGLGSVRFLMSVGDLVIGWLLQKQAAVAIAKLDDGASGA
                     DRDFYEGKIAVASFFAKNFLPLLTSTRVVLENIDNDVMEIPEAAF"
     gene            complement(14783..15535)
                     /locus_tag="VF874_13055"
     CDS             complement(14783..15535)
                     /locus_tag="VF874_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX9176956.1"
                     /translation="MAYQANMRVWRGDLEGGALQDYEVEVNEGEVVLDIIHRLQQTQT
                     PDLAVRWNCKAGKCGSCSAEINGRPKLMCMTRMSTFGEDEVVTVTPLRTFPVIRDLVT
                     DVSFNYEKAREIPSFAPPKELQPGEYRMQQEDVNRSQEFRKCIECFLCQNTCHVIRDH
                     DDNKKAFAGPRYLMRQAELDMHPLDTLNRRPEQAQDDNGLGYCNITKCCTEVCPEHIK
                     ITDNALIPMKERAVDRKYDPVVWLGSKLLRRK"
BASE COUNT         2538 a         5223 c         5350 g         2571 t
ORIGIN      
        1 ctcgtttccg tcgtcgtcga gcaccttcat ctcgccggcg accacgaccc ccaccgaacc
       61 gcgatgcgcc agccattgct cgccgctgac gaatgtcatc gcctgaagtt cggtgcctcc
      121 gtagagctcc cagagcactt caggcccgag caggtcgatc caggcctgct tgaccgcctg
      181 tgggcacggc gcgccgacgt gccagaaccg ccggatcgac gacaagtcgt aggcgtccgg
      241 gttcgcccgg tacgccgcat gcacgcgctg catgatcgtc ggcacggtca ccaggaagtt
      301 cacccggtga tcggtgacca gccgcagaaa gtcggccgcg tcgaatcgcg gcatcagcac
      361 cagatgctgt cgcatcagca ggcttatcgt cgccgcggtc atgccggtgt tgtggctcaa
      421 cggcaccgac accaggtggg tgtcgccctc ttcggcaccc atcatctgtc cgaaaatcgt
      481 tgccgcgaaa cgactgtcgc cgccagcctc gatgagtttc ggccgcccgg tgctgccgcc
      541 cggggccaag gccttccacg taggcgacac ggcctcgggc aacggaccgt cggaaagacc
      601 cggatcggga gtgaattcca ttgggaggct aggtagttcg ctgctgatcc ggccaacgat
      661 cagcgcccgc gaccgcagct gcatcaatgc gtcgaactca ggttcgggca aacgcgggga
      721 cagcggttgg ggaaccgcgc cgagcttcca cgtcgcgagc gcggcctgga tccattcgat
      781 cgaattcggc agcgcgatgg tgacgtagtc gcctacgccg acgccacggt cggcgtacgc
      841 gcgggccagc cgattggtcg agctgtcgag ttcgccgcgg gtaagggtcc gcccgtcgca
      901 ggtgaccgcc ggctcgtcgg gcgcgaccgc ggccaggcgg gagaactgcg ttccgatcgg
      961 cgggacggcc tcggtcatgt cggtacccgc gctggcgctc gaagatcttg cgcgggttgt
     1021 ccaccagcat ggtgcggatc tgctcgtcgg tgacaccgcg ctccttgagc gccgggatca
     1081 cgtcgtggtg gatgtgcagg taatgccaat tgggagcggc caccgacagc aggtcctcgg
     1141 gcagcgcgtc gaaatagcag ttcgcgtcgt gcgacaacac catcttgtcg gcatgcccgc
     1201 gttcgcacat ccgcgccacc gtgttcaccc ggtcctcgaa cggaaggatc acgtcgatgc
     1261 cgaaccggtc catgccgatg tagcttccgt tggcgatcag ctcctcgaga tagtcgatgt
     1321 ccgtcgagtc gccggagtgg ccgatgacca cgcgggtgag gtcgacgccc tcgtcggcga
     1381 agatgcgttg ctgctccaga ccacggcgtg tcggcgcgtg cgtgtgcgtc gagatcggca
     1441 cgccggtctg gcgatgcgct tgcgcgaccg cgcgcagtac tcgctcgacg cccggggtca
     1501 ccccgggttc gtcggtcgcg cacttgagga tcgcggcctt gagaccggtg tcggcgatgc
     1561 cctgctcgat gtcgcggaca aacaggtcgg tcatgatttc gggcccgtcg agcggcgcac
     1621 ccggcccgag gtagtggaaa cagaacggca cgtcgttgta ggtgtacagg cccgtggcga
     1681 cgacgatgtt gagatcggta gccgcggcga ctcgcgcgat ccgcgggatg tagcggccga
     1741 gtccgatgac ggtgaggtcg acgatggtgt cgacgccggt tgccttcagc tcgttgagcc
     1801 gcgcgatcgc gtcggcctcc cgcgcggcgt cctcacccca cgattccgga taattctgca
     1861 tgacctccgt cgtcatgatg aagacgtgct cgtgcatcaa agtcacaccg agatcgttgg
     1921 cgtcgaccgc gccccgcacc gtattcactt ctggcataac ggcagtctgc tacttgacgg
     1981 gcggttggcg gcgcgtgatc ggtacaaccg atgcggcgag cgcgccgacc gcgacgagcg
     2041 ccagcagctg gacaccccac gagtgcccga cgtagccgcc caccgagcgc cacgggtgac
     2101 gcgacagcac cgcgccggcc aggatgaacc cggtcgccgt cgtgccgagg gtgattgcgg
     2161 cggacaacga cggccggtca cgcagccagt accgcacggc gagcgctccg gcgaacacca
     2221 cgacgccggc gatcgcggag atcagccagc ccgctcccag caccgcgatc gtggccactg
     2281 cggtcggtag ccgccagggg cgtgcgggct cgtcgtcgac ttcgcggcgc ggccgcacca
     2341 gcgccagcag cgcaagcaac ggcagcagcg ccaatcccca gaacagcccg gcccggtaca
     2401 gcgtgttgga cgggaaggtc agcgtcacaa cacctgacgt gccggcgggc agcacccagc
     2461 cctgctgcca gccgttgacg acgacgggcg tcaaccgggt gccgtcggcg gtgtgcgcca
     2521 cccagcccgg gttcacgctt tccggcacga ccagtacccg cgctgtgggc gccggttcgg
     2581 cacggacctc gcggcggtcg ggcccccacg tgccgacctg cacgggaatg gccacggccg
     2641 cggcttgcgg tggcagcggc gtcgtcagtt gggcgccgtc gacaacgaac ccctcgccgg
     2701 ggcttatcag caactcctgc tcgcccgccg acagcgcgat cggctcgcgc tggcacggct
     2761 gcgcctcgat cggttctccg gccagcagcg cgctgaccgt ggtgtgcact gaggtctgga
     2821 cgaagcgccc tgcgatcgcg atcgtcgggc cgcggccgca tccgaggtcg atcgggcggg
     2881 cctgattgcg gtcggcgtcc gcgggcgcaa tcggcttgcc gtcggcgccg agcgcggtga
     2941 cctccgcgat gccgggcggc ttgagctggt cgaagccgag cgcggtgcgg tcgatgacgt
     3001 cgtcccagtc gagcacactg atcgtgaccg tgtcggtgat ccggggcgtc aatgtcagcg
     3061 tcctggtctc gccgtcgtcc cccgcaagcg ggtgggggta cctcccgttt acgggggagt
     3121 gcccccagtc gcgactcccc gacagtggcc gcacctgcgg gccgtcgccc aggtcgaccg
     3181 cgacgagcgt cgggtgcgtg ggcaccgacg acgcgctcgg aatcagctgc agcccagcca
     3241 cttccgtggg tcggggcagt ttcagcgtga gtgtgggcgg tgtgcggtcg tggacgacgt
     3301 tctgcggtgc gatccacgac gtgcgcggat cgccgtcggc ggccgcgaac gccgagcctt
     3361 gaatgtcgat gacgtcggcg tcgccgctgg cgcgggcggg gcccggctgc gcgaccacgt
     3421 ccgccagctt cgggccctgc cgtgcgcgca cccacaccgt cggcgtcacg gttaccggct
     3481 cgggcaccgt cagcgtgcgg ctcaagttca ccggctcctc gggggcgagt gccatcgttg
     3541 ctgcacagcg cacgcgatcg ggaccgtccg cgcagccgta gcggcccagc agttccgagc
     3601 cgagatccca gagcgccacc gcggagcctg ccggcggggc agggaccgcg acggtgtgcc
     3661 gcagggtcac cggatgagcg aatccgttcg cgtcgtactg ggtgatcgcg atgtcgatga
     3721 tcccgaactg caccccggcg gacccgtcgt cagtacccac cgcggtcacc cgcacccacg
     3781 gcgtctcccc gtacggaagc gccgcggtga ggggctttcc cggttcgtcg aaacgcaccg
     3841 tggtggtgcc ggtggcggtg gcgatctcca gcttgcgcac ctgcgccccg acggcggtgg
     3901 cgctcggggt gatcgtgatc gtcgcgttgg tgatcgggtt gtcgaagtcg acccgcagcc
     3961 attggccgac cgcggcctgc agggcgttgg acacccagct cgtcgccgtg tcgccgtcga
     4021 tcgcggcggc cgggctcgtc gccggcgaga cgtttggcag tgcggtggag tcggcggccg
     4081 agctcgatgc cgtcagcctg ccgccgaccc agtcgccgtg gacaggctgc gcgtcggggg
     4141 ggtagtcggg cacccggttg aacgtgtgcc gctcgtcgcc gggcgcgcgg atcgccgacg
     4201 agtggtcgtc gacgcggccg tagtcggttt cgcgggccag cggagtgtcg gtgacggtga
     4261 cgacgggcgc cggcagggcg gcgcgctgcg cgtcggcggt caatagtgcc gggcccagcg
     4321 gcggctcgcc ctgcagccgg cggcgctcgt tgagccgcag cagcgcctcc gggccgccgt
     4381 cgacgcgcgg catcgcgtcg atatcggcaa ggtacggccc tgcggctccg gggttgaatg
     4441 tcgccggccc tgcggctccg gtctccacgc ggtagatctc gaccgcggcg tacttcggcc
     4501 gcaggtcgct gtcggcgacg aaccccggca cggtcccggc gccgacggga tcgccgaact
     4561 gcgccacctt ggtcaggccc ggcgacccgt cgatcgcgcg gtgcacgagg atcggccgcg
     4621 ccgatctcga tgtgtccgga tcgaggtcgt tgcgcacggc gacatacgaa atcccttgct
     4681 gcgcaagagt atccatcaat ccagcggagg gccgccccgc ggcgagcagc cgctgcacgg
     4741 agtccagcgc tcggatggtc gccgcggggg tcagcggcac agagtcgcgc acaccccacg
     4801 ggctctcgcc aagcacctgc agcggctcgt cgtgactggt gccccacacc tgggtggcga
     4861 acggcgaccc gggcaccacc agcactcggc cgggtgtcgg gccgctgtcg ttgtgctcgt
     4921 cgagccagtc cgccgcgtca tgccaatact gcggaatcgc gctgaacgtg ccggggggcg
     4981 tgagccggcc ggtccaggcc agcgatgtgc tgaccgccag cgcgaccagc acgacgatcg
     5041 caaccgccac gcgcttgtca tgctcggggt gggccagcgc gtgcacccac actcggcgcg
     5101 gtaccgcgcc cggaagcggc acgcggccga gcagatgagc aagccccagc accagcggca
     5161 gccggatcac cggctcgagc ttgtgcacgt tgcgcagcgg cgccccggcc gcgtcgagga
     5221 acgcctgcac ctcgtgcgcc accggggagc cgagcccgcc ggagtaaccg accgcgagca
     5281 gcacgacacc gaccagcaac accgtcacca gccgaccgcg cgcaggcatc gaggacagcg
     5341 ccaggccggc cagtccgccc gctgcgacga gagtcgtggc caggatcacg accgagctgg
     5401 tgaccagcga ttgccccgcg gtggccgtgg gcgcgacgta cggtgtccag ctatcggtgc
     5461 cgcgcaatac ctcggtaagt gacgtccact gcgtcgtgac gccggacgat tcgatgaagt
     5521 cgaggaacgg cgggctcacc tggccgagca gcgccaacgc gaccagccac cacgtcgtcg
     5581 ccaacaggat gcccagcagc caccacccgg tgaaccgcca ccacagccgg ttcggtcggt
     5641 gacaggtcca ccagatgacc gcggccaggc agccggtgag cgtcgcgacc gcgttgaccg
     5701 cgcccatcaa cgccaccgcc accgacgcgc gcgcggccag cacccgtacg ttgcctgatc
     5761 cgcgcaaggc caggatcact ggcagcagca cccacggggc cagcatcatc ggcagcgttt
     5821 ccgacgaaat cgagccgagg gtcgtcagga ctcgcggcga cagcgcgaac gccgccgctg
     5881 cgattacccg agacgacgtg gtgccgatgc cgagcgcctc ggcgactcgc agcaggcccc
     5941 agaacccgac ggtcagcagc agcgcccacc acagccgctg cgtcaaccag ccggggaggc
     6001 ccagggtgtc cccgagaagg aagaacgcac cgtgcgggaa aaggtatcca taggcctggt
     6061 tctgggcctg accgaacggc agctcactgc tccacagatt cgtcgcgcgg gcgaggaacc
     6121 gcagcgggct cgcggtgaga tcgagcttgg tgtcgggtga cacacgcccg ggcgattggg
     6181 cgagacacag gatcagcgcg gcggacccga cggcccacag ccaacgccgt gacaatggcc
     6241 cgacggcaga ctcctggctc gtcgcagtgt ggctagctac ggttgccgta ctccacccgg
     6301 ttgagaatcg acgcaccgtc accgatcgac agcggcggtt tcttgtcctg ctgcaccatc
     6361 agcgtcacgc cgaagaccgc ggccgcgccc agaagcagac ccaacaccaa cgagctcgct
     6421 ccggccaacg cgaactggcc ccgcggtgaa ttcctggtca ggttccagta ggcgaccgcc
     6481 accccgaggg caatcagcag tacgacgccg cccacgatca acagggacca cgccagtgaa
     6541 ctcactgaat gactcctcgc gacgacggct ggatctgggc ctccgccaag ttagcagacg
     6601 cgatgcgctg caccgccgga gcgggaactc gccgtgttct gggtacgatc cggccatgcc
     6661 gtcaatcagc gggcgtactg cacgacatgg ccagcaccga tcgctgcggc ggggactacc
     6721 catcctgcag gtcgccttca ccgtcgcgct ggtcgccgcg atcaccttcc tcggcatctt
     6781 gtggttccgc caccgggaga cgacgaatgc caacgcggcg ccaccggcag ccacgagtgc
     6841 caggcaggcg agtaatcagc ccttgaccat gccgatcggg ccgcccgctg cgccggtctg
     6901 cagcgacgac gcgctgacgt cgatgccgat acgcgacaag ctcgcgcagc tgctgatggt
     6961 cggcgtgacg ggggccgatg acgcgcaggc ggtggtggcc aactaccacg tcggcggcat
     7021 catcatcggc agctggaccg acctttcgat gctgggcagc ctgggcgaga tcacgtccgc
     7081 cgggacgctt ccggtggctg tcggcgtcga cgaggagggt ggccgggtgt cgcggctgtc
     7141 gtcgctgatc ggggttgctc cttcggcgcg tgcgctggca gcgagcagta cgccggacga
     7201 ggtgtatgcg caggcgctcg cacgcggcca ggcgatgcgc ggattgggca tcacggtcga
     7261 cttcgcgcct gtcgtcgatg tcagtgacca gcccgacgac accgtgatcg gcgaccggtc
     7321 gttcagcgat gacccggcca cggtgacgga gtacgccggg gcatatgccc gcggcctgcg
     7381 cgacgccggc ctgctgccgg tgttcaagca cttccccggc catggccacg ggtccggcga
     7441 ctcgcacacc acctcggtgg tgacgccgcc cctgtcgcag ctggtgacga atgacctcgt
     7501 gccgtacaag acgttgctca atgagcagcc ggtcgccgtg atggttgggc acatgcacgt
     7561 gcccgacctc accggcggcc aaccggccag cctcagtggt gcggcgtaca cgctgctgcg
     7621 taccggcagc tacggcggac cgccgttcgg tgggccggtc ttcacggacg acctgtcgag
     7681 catgcgcgcg atcagcgacc gctacggcgt cgcgcaggcg gtgctgttgg cgttgcaggc
     7741 aggcgcggac atcgcgctgt ggatcaccac ggccgaggtg ccggcggtgc tcgaccggct
     7801 ggaacaggcg gtgaacgccg gtgagctcac ctcgcaggcg gtcgacgaga aggtgctgcg
     7861 cgcgacaggg ttcaagggcc gcaatccgca gtgctgaccc ggcacttact ctggattcag
     7921 ataaccgacc gggcggcgta aggactgacg atggcgggtg gcacgaagcg gttgccgcgc
     7981 gccgtgcgtg agcagcagat gctcgacgcc gccgtggaga tgttctcgcg caacggttac
     8041 cacgagacgt cgatggacgt tatcgccgcc gaagccgaga tctccaagcc gatgctgtat
     8101 ctgtactacg gctccaagga ggagctcttc ggggcctgcc tgaatcgcga gttgagccgg
     8161 ttcatcgagg cggttcgcgc tgacattgac ctcaagcaga gcccaaaaga gttgctgcgt
     8221 agcactattc gggcgttcat gcattacatc gacgctaatc gggcgtcgtg gatcgtgctg
     8281 taccagcagg ccaccagttc gcagatgtac tcacacaccg tgcttgaggg ccgcgagagg
     8341 atcatcgacc tggtggcgag gctgttgagc gccggtacgc gaaaccccga acccgacacc
     8401 gactttcaca tgatggccgt agcgttggtg ggcgccggcg aggcgatcgc gacgcgggtc
     8461 agcaccggtg aggccgacgt caacgaggca gccgagctgc tgagcaacct gacctggcgc
     8521 ggcctgaggg ggcgccctgt agacgaggat gtcacgccag cggctacagc gcgttcacgc
     8581 tcccggtgag gtgcggctcg cccttcgagt tgcgcagcgc caggtcccag ccgtcggcta
     8641 cccgcgacac gtacaggttg gcctcagcgg gcagcacgac gggcttgccg aaccgcacgg
     8701 agtaccgcac cgcgtcgggc aactgcccct cgatcgtccg cagaaccgct gccgccgtga
     8761 acatcccgtg cgcgatcacg gtcgggaagc cgaacagctt ggctgcaacc gggttggtgt
     8821 ggatggggtt gtgatccccc ccgaccgatg cgtagcgacg gatctgcctc gcgctcacgc
     8881 tcagaacacc attcggcggg ggcaacttcg gctgcttctt cggcggcggc ttgggctcgt
     8941 cggacaggct ggtgcgctgc tgatgcaaga acgtcgtcac ctgatgccag gccaggtcgt
     9001 tgcccacgct gacgtcggtg agcacgtcca ccagcaagcc gcgacggtgc tcacgcagat
     9061 tctcggcgtg caccgtgacg ttcaccgtgt cggttaccga gatcggccga tgctgggtga
     9121 tgtggttctc ggtgtgcacc gaacccattg cggcgaaggg aaagtcgaag ccggtgacca
     9181 atgacatcac caccgggaag gtgagcgcga aggggtaggt cagcggcacc tgatcggcgt
     9241 agcgcagccc ggtgacggcg gagtactgag cgacgttgga ccggtcgatc tgcaggccct
     9301 ccactttgag tgtgcgatcg ggcacgtcgt cgctgcgcgg gatgaacggc agggcgccgg
     9361 cggcggcgcg cagcaggttg cgcagcgcgc tgggccggtc ggtggtctcg acggtgctca
     9421 tgcgcgatcc gcctccgctt ctcgctcggt gctcatgcgc gatccgcctc cgcttctcgc
     9481 tcggtgctca cgtcaggcgc ccagcatcgc ctggccgcat acccgaatca cgttgccagt
     9541 gactgcattc gaggcggggc tggcgaagta cgcgatggtt tcggcgacgt cgacgggctt
     9601 gccgccctga tacagcgagt tcagtcggcg cgccacctcg cggatggcga acggaatggc
     9661 gtcggtcatc gccgtctcga tgaaccccgg cgcgaccgcg ttgatcgtga tgcccttgtc
     9721 gtacagcacc ggcgcgagcg cctgcgtgat gccgatcatg cccgccttgg tggtcgcgta
     9781 gttcgtctgg ccccggttgc ctgcgatacc ggccatcgac gacagaccga ccacccggcc
     9841 gccctcgccg atggatccgt tgtccaccaa gccctgcgta agacgctgtg gcgcaaggag
     9901 attcacagcc aggaccgcgc tccagcgggc gtcgtccata ttggccagca gcttgtcgcg
     9961 ggtgataccc gcgttgttga ccaaagcgtc tgcgcgtccg tcgtagtgtt cacgcagatg
    10021 ctcggtgatc cggtcgaccg cgtcgtcggc ggtgacatcc aatgtgagcg cagtgccccc
    10081 caccttcgtc gcggtctcgc ccaacgcttc ttgggacgct tcgacgtcga cggcgatcac
    10141 ccgggccccg tcgcgggcga acacctcggc gatcgtggca ccgatcccgc gggccgcacc
    10201 ggtgacgatc gcgaccttgc cgtccagcgg cttgtcccag tccgcaggcg gagtcgagtc
    10261 ggcctcactg acatagaaca cctgtccgtc cacataggcg gatttgccgg ataggaggaa
    10321 ccgcatcgtc gactcgaggc cggtggcggc gggcttggcg ttcggcgaga cgtacaccag
    10381 ctgcattgtg gcgccgtggc gcagctcctt ggccagtgat cgggtgaatc cctccagcgc
    10441 gcgctgggcg atgtgctcat cgacgctgcg cgccgcatcc ggtgtggtgc cgacgacgac
    10501 gacgcgggcg gagtggccga ggttgcgcag cagcggcggg aagaactcgt acagcgcgtt
    10561 gaggccctgc ggctcggtga tgccggtggc gtcgaacacc aggccgccga acgagtcggc
    10621 ccagcggccg ccgatgttgt tgcgcaccag ctcgtaatcc tcggccagcg cgctgcgcaa
    10681 cggttcgacg acgcgcccct cgccgccgat gagaagtgag ccggcgatag gcggttcgcc
    10741 cggcttgtag cgacgcagcg gctccggctg ggggatgccc aactgcttgg ccaagaacga
    10801 cccaggtgtc gagttgacga cttgggtgaa caggtcggaa gccacggaag tgcctttcgt
    10861 gtcggtagtc aggatgaact tactccgcag taaggaggat gtttactatg gggtattcat
    10921 agccggagag acagtggagg caaacgtggc gacgaaaaaa gccagccggc gggtggcagt
    10981 tctgggcggc aaccgcatcc cttttgcgcg ctcagacggc gcttacgcgg acgcgtccaa
    11041 ccaggacatg ttcaccgccg cgctgggcgg gctcgtcgat cgcttcaacc tcgaggggga
    11101 gcggctgggc gcggtgatcg gcggtgcggt gctcaagcac agccgtgact tcaacctgat
    11161 gcgcgaatgc gtgctcggta gcgcgctgtc gtcgtacacc ccggccttcg acctgcagca
    11221 ggcgtgcggc acagggctgc aggcgacgat cgcggcggca gacggcgtcg gcgcgggccg
    11281 ctatgaggtc gccgcggccg gtggtgtgga caccacctcg gacgcgccga tcgctattgg
    11341 tgacaacctg cgccggaccc tgctcggatt gcgccgggcc aagtccaacg tcgaccgcct
    11401 caagctggtc ggaaagctgc ccgcgacgct gggcgtcgag atccccgtga acagcgagcc
    11461 gcgcaccgga ctgtcgatgg gcgagcatca ggccatcacc gccaagcaaa tgggcatcgc
    11521 gcgcgtcgcc caggacgagc tggcggccgc gagccaccgc aacatgagcg cggcctacga
    11581 ccgcgggttc ttcgacgacc tggtgacgcc gttcctgggg gtataccggg acaacaacct
    11641 gcgcccggac gcgtcggtgg agaagctcgc gaagctcaag ccggtgttcg gggtgaaggc
    11701 cggcgacgcg acgatgacgg ccggcaactc gacgccgttg acggacggcg cgtcagtggc
    11761 gttgctggct agcgaagatt gggccgagca gcattcgcac gagccgctgg cctatttcgt
    11821 ggacgccgag acggcagggg tcgactacgt caacggtccc gacgggttgt tgatggcgcc
    11881 gacgtacgcg gtcccgcggc tgctggctcg caacgggttc agcctcggtg acttcgactt
    11941 ctacgagatc cacgaggcgt tcgcgtcggt ggtgctgtgc cacctgcagg cttgggagtc
    12001 ggaggagtac tgcaaagagc gcctgggcct ggacgccgcg ctcgggtcga tcgaccggtc
    12061 caagctcaac gtcaacggca gctcggtcgc ggcggggcac ccgttcgccg cgaccggcgg
    12121 acgcatcgtc gcgcagctgg ccaagcagct ggcggagaag aagaaggaca ccggcgggtc
    12181 ggttcgcggg ctgatttcga tctgcgctgc gggcggccag ggcgtggcgg cgatcctcga
    12241 agcctgagca gcctgccata gaaattctcc ggcgggatgt aacgcacccg tgtatgcagc
    12301 cgatattcgg gtagccccgt gttgccgtct gaccccccga cccgacgaca acacggggta
    12361 cctctacccg gagatggact tgaaccgccg ttggcaagag gggggccaac ggcggttcgt
    12421 tttcttccct atagatctgg tagcgtagcg gtttcgggac tactatcgtc agcacgacgg
    12481 gttcgggaga tgactgatcc gaatagccgg tgtccgaggt gaaaagccgg tagcgcagga
    12541 taagcgagac gccctcgagt gtcaccccga acccgccttt tattgtctgg actatgacac
    12601 gcgaaagccc ccgtcccaca agggaatcgg gggcttcggc gttgacggtc agaaggccgc
    12661 ttcggggatc tccatcacgt cgttgtcgat gttctccaga accacgcggg tgctggtgag
    12721 cagcggcagg aagttcttcg cgaagaacga ggccaccgcg atcttgccct cgtagaagtc
    12781 gcgatcggca ccgctggcac cgtcgtccag cttcgcgatg gctaccgcgg cctgcttctg
    12841 cagcaaccag ccgatgacca ggtcgccgac cgacatcagg aaccgcacgg agccgaggcc
    12901 gaccttgtaa aggctggtcg agtcctcctg cgcggccatc aggtagccgg tcagcgtcgc
    12961 ggccatggcc tgcacgtcct gcagagcggt ttcgagcagc gcacgctcgg ccttgagccg
    13021 gccgttgccg tactcgttct tgacgaactg ctcgatctgg ccggccacgt gagccaaagc
    13081 cacacccttg tcgcggacga tcttgcggaa gaagaagtcc tgcgcctgga tggccgtggt
    13141 gccctcgtag agcgagtcga tcttggcgtc gcggatgtac tgctcaatcg ggtagtcctg
    13201 caagaagccc gaaccgccga acgtctgcag gctctcggtc agcttcgcgt aggcctgctc
    13261 ggagcccacc cccttgacca ccggcagcag caggtcattg acctgcactg ccagcttggg
    13321 gtcgataccg tgcacggcct tggcaacctc actgtcctgg aaggtggcag tgaagatgta
    13381 cagcgcacgc agaccctcgg cgtaggcctt ctgcgtcatc aggctgcgcc gcacgtcagg
    13441 atgatgagtg atcgtcacgc gcggcgcggt cttgtccgtc atctgggtca tgtcggcgcc
    13501 ctgtacgcgg ctcttggcgt actcgagcgc gttgaggtaa ccggtcgaca gcgtcgcgat
    13561 ggccttggtg ccgaccatca ttcgagcctg ctcgatcacg tcgaacatct gggcgatgcc
    13621 gttgtgcacc tcgccgacga gccagccttt cgcggggacg ccgtgctggc ccagcgaaag
    13681 ctcgcaggtc gcggaggcct tcaggcccat cttgtgctcg acgttggtga cgtatatgcc
    13741 gttccgctca ccgatctcgc ccgtctgcgg gtcgaacagg aacttcggca cgaagaacag
    13801 cgacagaccc ttggttcccg ggcctgcgcc ctcggggcgg gccagcacca agtgaaagat
    13861 gttctcgaac agatcgcccg agtcgccgga ggtgatgaat cgcttcacgc cgtcgatgtg
    13921 ccaggacccg tcgtcctgct tgacggcctt ggtgcggccg gcgccgacat cggaacccgc
    13981 gtcgggctcg gtcaacacca tcgtgccgcc ccagccgcgt tctgcggcca acacggccca
    14041 cttcttctgc tcctcggtgc ccaggtggta gacgatctgg gcgaagccgg caccgccgcc
    14101 gtacatccag accgcggggt tggcgcccaa aatgtgctcg tgcagcgccc ataccagcgc
    14161 cttcggcatc ggcatgccgc cgagcgcctc gtcgatgccg accttgtccc aatggccgtc
    14221 gacgagcgcc cgaaccgact tcttgaacga ctccggcaag gtcaccgagt gcgtcttggg
    14281 gtcgaaggtc ggcggattgc ggtcggactc ggtgaacgag tcggcgatcg ggccctcggc
    14341 gagccgggcc atctcggcga gcatgtcgcg ggcggtgtcg ccgtccaggt cgctgtactc
    14401 cccctggccg agcgccttat caatgtcgaa cacgtcgaac aggttgaact cctgatcgcg
    14461 gacgttgctc ttgtagtgac tcacggttcc tcctcgttga gagcgccgca cgtggttggg
    14521 tacttgctgc caagttaccc accagtaact ggcataacta taccgctcgg taacttattc
    14581 gcaaatcgct ttgttagcta actaacatac cggttggtcg agcgcccagc accaccctga
    14641 cctgcgcgga tgggcgtgcg tcgagccggc gaggtatccg tatttcatgc cgccgacgac
    14701 cgtcggcagg gccgtcggct aaccggcggt tatcgcgtcg tgaccgagag gacaacgtag
    14761 acggctgggc gccgaataac tcttacttcc gcctgagcag cttgctgccc aaccacacca
    14821 ccgggtcata cttgcggtcc accgcgcgct ccttcatcgg gatcagcgcg ttgtcagtga
    14881 tcttgatgtg ttcggggcag acctcggtgc agcacttggt gatgttgcag taccccagac
    14941 cgttgtcgtc ctgcgcctgt tccggacggc ggttcagtgt gtccagcggg tgcatatcga
    15001 gttccgcctg gcgcatcagg taacgaggtc cggcgaacgc cttcttgttg tcgtcgtggt
    15061 cgcggatcac gtggcaggtg ttctggcaca agaagcactc gatgcacttg cggaactcct
    15121 gcgagcggtt gacgtcttcc tgctgcatcc ggtactcgcc aggctgcagc tccttcggcg
    15181 gcgcgaacga cgggatttcc cgcgcctttt cgtagttgaa cgacacgtcg gtgaccagat
    15241 cacggatcac cgggaaggtc cgcagcggtg tgacggtgac gacctcgtcc tcgccgaagg
    15301 tcgacatccg ggtcatgcac atcagcttgg gtcggccgtt gatttcggcc gagcacgagc
    15361 cgcacttgcc cgccttgcag ttccaccgca cggcgaggtc gggtgtctgc gtctgctgca
    15421 ggcgatggat gatgtcgagc accacctcgc cctcgttgac ctcgacctcg tagtcctgca
    15481 gtgcgccgcc ctcgagatcg ccgcgccaca cgcgcatgtt cgcctggtag gccatcactt
    15541 gctccgttct ggatgctggg ccagttcccc gtcggtgaag tacttctcca attcggagag
    15601 ctcaaacagg tcgagcagat cctgacgcac aggaatctgg tcctgcttgg tgacgctcac
    15661 gtcgggcacc agagcttccg gg
//
LOCUS       DASWBF010000354         6250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_361902,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000354 DASWBF010000000
VERSION     DASWBF010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361902"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VF874_13060"
     CDS             <1..1016
                     /locus_tag="VF874_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HEX9176957.1"
                     /translation="TQLGVVAWPSTRQDRLFASWNYWWQAHLLDCLVDAQVRDPQPQR
                     CKRIAAQIRGHHLRNIGRWTNSYYDDMAWMALALERSGRLAGVERQRALRTFTKQFTR
                     AWMPEFGGGIPWRKQDRFFNAPANGPAGIFLARNGELRQAQMMADWIDETLIDPETHL
                     VFDGIKEGSLDRAMFTYCQGVVLGLETELAVRTRDDRHRPRVHRLVAAVAEYMSNDGV
                     LKSEGGGDGGLFGGVTIRYLALVASALPGDTTEDVAARDTARRLVMTSAQSAWDFRQS
                     VDGLPLFGAHWDRTAEVPSAESGSATFTGGAVMASAMPERDLAVQLSGWMLMEAAHTL
                     TAE"
     gene            1013..3595
                     /locus_tag="VF874_13065"
     CDS             1013..3595
                     /locus_tag="VF874_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9176958.1"
                     /translation="MTVRANDPLTGRDDELAFIRRALGAGGKYAGVVVVGAAGVGKTR
                     LGREVMSRAEAVGERTDWIVGTESARSLPLGAFANLLSDPMAEPLPNVRRIINSFVAQ
                     RQRGRALIGVDDAHLLDGLSAHVVHQLAQTRGIRLVVTLRSGADEPDAVTALWKDGLL
                     ARLDLEPLSVQATRGVIEADLGGPVDTRCAQRFWKLTGGNALFLRQLVKDYVAAGRFR
                     QVAGVWMADDRAVASPSVTEMVEREMGRLTPGVASVVDVLSQAEPLPVDVVCDVAGRA
                     DLEGAEQQRLVTVERTGGKLMVRLAHPLFGEVRRATAGEMYLSRIRGRLAQRLAKDGD
                     ADVYATVRRALLTLESDLPPDPQLFLDSARSAMTLLDLDLADRFARAAADAGATEATG
                     LLAMNLALAGRGDEAEAVLSAMTDDGDHWATVRAANLTWMLARPSDAAALLAELDESP
                     ERLAIEACVEVVSARCRSAEQKARAALASGTLSDFHAMVASVALTMAMGALGHVDDLP
                     AVAEEALRRATTSFQASPMRFWFAGVYARACRLTGQITEFVAAAEKLAEGARDLPGLA
                     YANLALLLGNAALMRGDVAGAVRLLREALAGAENHSVTTGLRAASCFALAEAYAKLGQ
                     PDEATAALAEVRVPDKYLYMHTGLAVAKGWALAAGGALSEAVAVVQAAASDARDRDQP
                     THELACIQAAAQWGDGFGAPRARALANELSLPLANTVARHAETLQTGDGEGLLAVSAD
                     YRAIGDRAAAADAAAQAAAVFIQGQHSSRGSYSAAIARQLAEECGGLCTPALRSRLGI
                     PLTGRQRDVIELVASGLSNREIADRLVMSVRTVEGHVYRACQRVGATTREELAAILRG
                     GGGQ"
     gene            3592..4707
                     /locus_tag="VF874_13070"
     CDS             3592..4707
                     /locus_tag="VF874_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9176959.1"
                     /translation="MTACDSCGTDLPASAKFCLECGAPIALSHSQAEYKQVTVLFADV
                     VHSMDIAAAVGAERLREIMSELVHRCSKVIRRYGGTVDKFTGDGIMAVFGAPVALEDH
                     AFRACLAALAIQQDATALAAEVERSDDVHLQLRVGLNSGEVIAGEIGPGALGYTAIGE
                     QVGMAQRMESAAPPGGVMLSESTARLVEDVAVLGDPEEVRIKGAATPVSARRLLAAAQ
                     HSRIVPHRSTFVGRDWELAALTAMLDRSMNGHGCVASVVGPPGIGKSRIVAEVSAHAV
                     NSGVQVFSTAPLTWPNPSRACATRRSRLRVWPAATGQRRGKRMKRYANAAACIRRSGA
                     STVGRPSRRWRAAISPRHAAGQMMPCRSRRAITCRLR"
     gene            complement(4636..4947)
                     /locus_tag="VF874_13075"
     CDS             complement(4636..4947)
                     /locus_tag="VF874_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176960.1"
                     /translation="MVGVESLESHGAHPQAGGMGRTEESGRFSVASGKAGDLGKALQD
                     VRNDGVALAGDDAQCVAGVALGLFVVTLRNLDARASSAQPTSNSPSRSTRHHLPSRVP
                     R"
     gene            4976..5248
                     /locus_tag="VF874_13080"
     CDS             4976..5248
                     /locus_tag="VF874_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9176961.1"
                     /translation="MGENQFETAWAKGGALPIEEAIAYAQRGRGERKRPSSAWASLTP
                     TEHDVVRLVSEGLGNKDIAARLFMSHRTVQTHLTHVYNKLGLTSRV"
     gene            complement(5295..6197)
                     /locus_tag="VF874_13085"
     CDS             complement(5295..6197)
                     /locus_tag="VF874_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9176962.1"
                     /translation="MRGSKILITGPTGQVATPIAKALAIDNEVWGIARFTDAAARERL
                     EKAGVRCETVNLAAGDFAGLPSDFDYVVNLAVAKSGDWDKDLGANAESVGLLMAHCRG
                     AKAFLHCSSAAVYDPPDDDTRTERAALGDNHRFLFPTYSISKIAGEVVARSTARVLGV
                     PTTIARLNVPYGDNGGWPYYHMEMMLAGIPIPVPPGEPARYNPIHEDDIIATIPKLLE
                     VASVPATTVNWAGDQIVSLQEWCTYLGSLVGREPVFEESEHALRGNPVDVTRMHELVG
                     GTMVDWHDGMRRMAAKFHPELVRS"
BASE COUNT          973 a         1937 c         2293 g         1047 t
ORIGIN      
        1 gaacgcaact cggtgtggtg gcgtggccgt cgacgcggca ggaccggttg tttgcgagct
       61 ggaactactg gtggcaggcg cacctgctgg actgtcttgt cgacgcgcag gtgcgcgacc
      121 cccagccgca gcggtgcaag cgcatcgccg cgcagatccg cgggcaccac ctgcgcaaca
      181 tcggccgatg gaccaacagc tactacgacg acatggcgtg gatggcgttg gcgctcgagc
      241 ggtccggccg gctcgccggt gtcgagcggc agcgcgcgct gcgcaccttc accaaacagt
      301 tcacccgcgc gtggatgccg gagttcggcg gcggcatacc ctggcggaag caggaccggt
      361 tcttcaacgc cccggcgaac ggcccggccg gaatcttctt ggcgcgcaac ggagaactgc
      421 gccaagccca gatgatggcg gattggatcg acgagacgct gatcgatccg gagacgcacc
      481 tggtgttcga tgggatcaag gagggctcac tcgaccgagc tatgttcacc tactgccagg
      541 gtgtggtact cggcttggag accgaacttg cggtgcgcac ccgcgacgac cggcatcggc
      601 cgcgagtgca tcggttagtg gcggcggtgg cggagtacat gtccaacgac ggcgtgctga
      661 agagcgaggg cggcggagac ggcgggttgt tcggcggcgt gacgattcgg tacctggcgc
      721 tggtggcgtc cgcgctgccg ggcgacacaa ctgaggacgt cgctgcgcga gacaccgcgc
      781 gcaggctggt gatgacgtcg gcgcagtcgg cgtgggactt ccgccagagc gtcgacgggc
      841 tgccgctgtt cggcgcgcac tgggaccgca ccgcagaggt gccgtctgcc gagagcggga
      901 gcgcgacgtt caccgggggc gcggtgatgg cgtcggcgat gcccgagcgc gacctggccg
      961 tgcagctgtc gggatggatg ctgatggagg cggcgcacac gctgaccgca gaatgactgt
     1021 ccgcgccaac gatccgctga ccgggcgaga cgacgaactc gctttcatcc gtcgtgcgct
     1081 cggcgcgggt ggcaagtacg cgggtgtcgt ggtggtgggc gccgcggggg tcggaaagac
     1141 ccggctcggc cgagaagtga tgtcccgcgc cgaggcggtc ggcgagcgca ccgactggat
     1201 cgtcggcacc gagtcggcac ggtcacttcc gttgggcgcc ttcgcgaact tgctgtccga
     1261 cccgatggcc gagccgctgc cgaacgtgcg tcgcatcatc aattcctttg tcgcacagcg
     1321 ccaacgaggc cgggcgctga tcggcgtcga cgacgcgcac ctgctggacg gcttgtcggc
     1381 gcacgtcgtg caccagttgg cccagacgcg cggcatccgc ctcgtcgtca cgttgcgcag
     1441 cggagccgac gagcccgacg cggtgacagc gctgtggaaa gacggtttgc tcgcccggct
     1501 cgacctcgag ccgctgtccg tgcaggccac ccgcggcgtg atcgaggccg acctcggcgg
     1561 acccgtcgac acccgctgcg cgcagcggtt ctggaaactg accggcggca acgctttgtt
     1621 ccttcggcag ctggtcaagg actacgtggc agccgggcgg ttccggcagg tggccggggt
     1681 gtggatggcc gacgacaggg ccgtggcgtc gccgagcgtc accgagatgg tggagcgtga
     1741 aatgggccgg cttacgccgg gcgtggcgtc ggtggttgac gtcttgtcgc aggccgaacc
     1801 tctgcctgtc gacgtggtgt gcgatgtggc gggccgcgcc gacctcgagg gcgctgagca
     1861 gcagcggttg gtcaccgtcg agcggaccgg cggcaagttg atggtccggc tggcgcatcc
     1921 gctgttcggc gaggtgcggc gcgcgaccgc cggcgagatg tacctgtcga ggattcgggg
     1981 ccgactcgcg cagcggctgg cgaaggacgg cgacgccgac gtctacgcca ccgtgcgccg
     2041 cgcgctgctg accctggaat cggacctgcc gccggacccg cagctgttct tggactccgc
     2101 gcggtccgca atgacgttgc ttgatctcga cttggccgac cggttcgccc gcgcggcggc
     2161 ggatgcgggt gcgaccgagg cgacggggct tctcgcgatg aaccttgcgc tggcaggccg
     2221 cggcgatgag gccgaggccg ttctgtcagc catgactgac gatggtgacc actgggcgac
     2281 ggtgcgggcg gccaacttga cgtggatgct ggcccggccc tctgacgccg cggcccttct
     2341 cgccgaattg gatgagtccc ccgagcgttt agcgatcgag gcgtgcgtcg aggtcgtgtc
     2401 ggcccgctgc cgaagtgcgg agcaaaaggc cagggccgca ctggcttccg gaacgttgag
     2461 tgattttcac gcgatggtgg catcggttgc gttgaccatg gcgatggggg cgctgggtca
     2521 tgtcgacgat cttccggccg tggccgagga agcacttcgc cgtgcgacga cgtcgtttca
     2581 ggcgtcgccg atgcggttct ggttcgccgg ggtgtacgcg cgggcgtgcc gactgaccgg
     2641 ccagatcacc gagttcgtcg cggcggccga gaagctggcc gagggggcgc gcgaccttcc
     2701 cggcctcgca tacgccaatc tcgcgctgct gttgggcaat gcggcgttga tgcgcggtga
     2761 cgttgcaggt gcggtgcggt tacttcgtga agcactcgcg ggtgccgaaa accacagtgt
     2821 gacaacaggt ctacgcgcag caagctgttt cgcgctggcc gaggcatatg ccaagctggg
     2881 tcagccggac gaggccaccg ccgcgcttgc cgaggtgcga gtgccggata agtacctgta
     2941 catgcacacc gggctggcgg tggccaaggg gtgggcgctg gcggccggtg gtgcgctgtc
     3001 cgaggccgtg gcggtcgttc aggcggctgc gtccgacgcg cgggaccgcg accagccgac
     3061 gcacgagctg gcctgcatcc aggcggcggc gcagtggggc gacggtttcg gggcgccgcg
     3121 cgcacgtgcg cttgcgaacg agttgtcgct tccgctcgcg aacaccgttg cccgccatgc
     3181 cgaaacgcta cagaccggtg atggcgaagg gttgctcgcc gtgtcggcgg actaccgggc
     3241 gatcggcgac cgggctgccg ccgccgacgc ggccgcccag gccgctgctg tgttcattca
     3301 gggccagcac agcagcaggg gttcctactc cgcggcgatt gcgcggcaac ttgccgaaga
     3361 gtgcggcggg ttgtgcacgc ctgctttgcg tagccggctg ggtataccgc tgacgggccg
     3421 tcagcgggac gtgatcgaac tggtggcgtc ggggttgtcg aaccgggaga tcgccgaccg
     3481 gctcgtgatg tccgtgcgca ccgtcgaagg ccacgtgtac cgagcgtgtc aacgggtagg
     3541 cgcgaccacg cgagaggagc tggcggccat cttgcgtggc gggggcgggc aatgacggcg
     3601 tgcgactcgt gcggcaccga cttgcctgcc agtgcgaagt tctgcctcga gtgcggtgcg
     3661 ccaatcgcgc tgtcccacag ccaggctgaa tacaaacaag tgacagtgct gttcgccgat
     3721 gtggtgcatt cgatggacat cgccgcagcc gttggcgccg agcggttgcg cgagatcatg
     3781 tcggaattgg tccaccgctg ctccaaggtt attcggcgct acggcggcac ggtcgacaag
     3841 ttcaccggcg acggaatcat ggctgttttt ggggcgccgg tggcattgga ggaccacgcg
     3901 tttcgtgcct gcctggcagc tttggccatc caacaggatg cgacggcatt ggccgctgaa
     3961 gtcgagagaa gcgacgacgt gcatctgcag ctgcgagtcg gcttgaactc gggtgaggtg
     4021 atcgcaggcg agatcggccc tggcgcattg ggttacaccg cgatcggtga acaggtcggg
     4081 atggcgcagc gaatggagtc ggctgcgccg cctggtggag tgatgctcag cgaatcgacg
     4141 gcgcgactcg tggaggatgt cgcggtgctc ggtgaccctg aagaggtccg tatcaagggt
     4201 gcggccactc cggtatctgc gcgcaggctg ctggcagcag cgcagcacag tcggatcgtc
     4261 ccgcaccggt cgactttcgt ggggcgcgac tgggagctag cggcgctgac cgccatgctg
     4321 gatcgctcga tgaacggaca cggctgtgtg gcaagcgtgg tggggccacc tggaatcggg
     4381 aagagtcgca tcgtcgctga ggtgtccgcc cacgcggtta actctggcgt gcaggtgttt
     4441 tcgacggctc cgctgacatg gccgaatcct tcgagggcgt gtgccactcg gcggtcgcgc
     4501 ttgcgtgttt ggccagcggc gaccggacag cggcgtggca agcgtatgaa gcggtacgcg
     4561 aacgcagcgg cgtgcatccg gcggtcgggg gcatctactg ttggtcggcc ctcgcgccgc
     4621 tggcgtgcgg cgatctcacc gcggcacgcg gctgggcaga tgatgccgtg tcgatcgcga
     4681 agggctatta cttgtcggct gcgctgatga cgcgcgcgcg tcgagattgc gcaaggtaac
     4741 cacgaacagg ccgagtgcga cgcccgcgac gcattgggca tcgtcgccgg ccagggccac
     4801 accgtcattc cggacatcct ggagtgcctt gccgagatcg cctgccttgc cggaagccac
     4861 cgagaagcgg cccgactctt cggtgcggcc catgccgccc gcttgcggat gggcaccgtg
     4921 cgattcaaga ctctcgacgc caaccatgat gcctgcgtta cgacccttcg aaaagatggg
     4981 cgagaatcag ttcgagaccg cctgggccaa aggcggtgca ttaccaatag aagaggcgat
     5041 cgcctacgca caacgtggcc gcggtgaacg caagcgaccg agcagcgcgt gggcatcgct
     5101 gaccccgaca gaacacgatg tcgtgcggct cgtcagtgaa ggtctaggca acaaggacat
     5161 cgccgcaagg cttttcatgt cgcatcgaac cgtgcagacc cacctcacgc acgtctacaa
     5221 caagctgggc ctgacgtccc gggtgtaact cgcccaggag gcggcgcgcc acggctaggc
     5281 cgcgtcatcc gacctcacga gcggaccagc tccgggtgga atttggccgc catccggcgc
     5341 attccgtcgt gccagtcgac catggtaccg ccgacgagtt cgtgcatccg ggtcacgtca
     5401 acgggatttc cgcgcagcgc atgctcgctc tcctcgaaca ccggctcccg gccgaccagt
     5461 gagccgagat aggtacacca ctcctgaagg ctgacgatct ggtcgccggc ccagttgacc
     5521 gtggtcgccg ggacggacgc cacttcgagc agcttcggga tggtcgcgat gatgtcgtcc
     5581 tcgtggatcg ggttgtagcg ggccggttct ccgggcggga ctgggatcgg gatcccggcc
     5641 agcatcatct ccatgtggta gtacggccag ccgccgttgt cgccgtaggg gacgttcagg
     5701 cgggcgatcg tggtgggcac gccgagaacg cgcgcggtcg accgggcgac gacctcccct
     5761 gcgatcttcg agatggaata cgtgggaaac aagaacctgt ggttgtcgcc caaggcggcg
     5821 cgctcggtcc gggtgtcgtc gtccggcggg tcgtagacgg ccgccgagga gcagtgcagg
     5881 aaggccttcg cgccacggca gtgggccatc aggaggccga ccgactccgc gttggcgccg
     5941 aggtccttgt cccagtcgcc gctcttggcc accgccaagt tgacgacgta gtcgaagtcg
     6001 gacgggagcc cggcgaagtc cccggcggcc aggttgaccg tctcgcaccg aacccccgcc
     6061 ttctcgaggc gttcacgcgc cgcggcgtcg gtgaagcggg cgatgcccca gacctcgttg
     6121 tcgatcgcca gagccttggc aatgggagtg gcgacttggc cggtgggacc ggtgatcaga
     6181 atcttggagc cgcgcatcgg ctgatggtat gccgcgctag gcccgttttc cgcatagttg
     6241 ggggttaagg
//
LOCUS       DASWBF010000355         4168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_100182,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000355 DASWBF010000000
VERSION     DASWBF010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100182"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF874_13090"
     CDS             <1..229
                     /locus_tag="VF874_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176963.1"
                     /translation="FTEVDKLLHRLDMPHSLKEHGIAEEEFVAALPELAMTAFEDLSN
                     RTNPRMPLVSEITALLKLGYYGSEDDAGSRG"
     gene            237..533
                     /locus_tag="VF874_13095"
                     /pseudo
     CDS             237..533
                     /locus_tag="VF874_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
     gene            551..1342
                     /locus_tag="VF874_13100"
     CDS             551..1342
                     /locus_tag="VF874_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HEX9176964.1"
                     /translation="MSNPRDMFAPLIEQHPGGPKVRRFRLGDPLSIVLIVVIVVALVA
                     ASLLGAELYARHRADSAVAAATECVVQDNASVSFGASPFLLQHMTGHYGNISITTAGN
                     QIRKAKGMKAQVSIDDVRLQSTANSKGTIGALDATITWSSDGIKQTVQNSIPLFGSFL
                     SGVKTNPSDGTIQLQGTLGSITAKPQVGNNGIGLQVVNLNGLGFTLPSESVQPALDAF
                     ANELTNSYPLGIHADSVQVTDSGVVGHFSTRNASIPQGNPCFANL"
     gene            complement(1407..1661)
                     /locus_tag="VF874_13105"
     CDS             complement(1407..1661)
                     /locus_tag="VF874_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176965.1"
                     /translation="MTHGNVTSPNVLGVPVFWSPYVNTGHIWAIPQAKSFVVIRDNPS
                     VVADASAYFSSDRVGIRCTARFEFAHPHPWGIVRIGIGGS"
     gene            complement(2190..2387)
                     /locus_tag="VF874_13110"
                     /pseudo
     CDS             complement(2190..2387)
                     /locus_tag="VF874_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            2509..3885
                     /locus_tag="VF874_13115"
                     /pseudo
     CDS             2509..3885
                     /locus_tag="VF874_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
     gene            3882..>4168
                     /locus_tag="VF874_13120"
     CDS             3882..>4168
                     /locus_tag="VF874_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9176966.1"
                     /translation="MTRVLVIDDEPQILRALRINLSVRGYEVITAATGAAALRVAAEH
                     RPDVIVLDLGLPDMSGIEVLEGLRGWLSAPVIVLSARIDSADKVEALDAGA"
BASE COUNT          731 a         1349 c         1403 g          685 t
ORIGIN      
        1 gtttactgaa gtggacaagc tgttgcatcg gctcgacatg ccgcactcgc tgaaggaaca
       61 cggcatcgcc gaggaggagt tcgttgcggc gctgccggaa ctggcgatga ccgcgttcga
      121 ggatctgagc aaccggacca acccgcgaat gccgttggtc agcgaaatca ccgcgctgct
      181 gaagctcggc tactacgggt cggaagacga cgcaggtagc cgcggctagc tccgacatcg
      241 agaagtggga tcccggcctc actgaacacc tggtcgggct gacccggccg atcgtcaaaa
      301 ggtacttccg ctccgaggtt ctgggcttgg acaacatccc gcccggcggc tcgttgatcg
      361 tctcgaatca ctccggcggc atggtcacgc tggacctgtc ggtgttcgcg acggacttct
      421 acgacaagtt cggctacgac cggccgctgt acgccctcgg gcacgacatg ctgttcacgg
      481 gtccggccgc cgagttcttc gagcgcaccg gggtcatccg cgcgacacgg gacaagacac
      541 tgtcatcaac ttgagcaatc cgagggacat gttcgctcca ctgatcgagc agcacccagg
      601 gggcccgaaa gtgcgacgct tccgccttgg cgatccgctg tcgatcgtcc tgatcgtcgt
      661 catcgtggtg gccctggtcg ccgccagcct cctcggcgcg gagctgtacg cccggcaccg
      721 cgccgacagt gccgtcgcgg cggccaccga gtgcgtggtg caggacaacg cgagcgtgtc
      781 gttcggggct agcccgtttc tgctgcaaca catgaccggc cactacggca acatctcgat
      841 cacgaccgcg ggcaaccaga tccgcaaggc caagggcatg aaggcccagg tgagcatcga
      901 cgacgtgcgg ctgcagagca ccgccaactc aaagggcacg atcggggcgt tggacgccac
      961 gatcacctgg tcgtcggacg gcatcaagca gaccgttcag aactcgatcc cgttgttcgg
     1021 cagcttcctc agcggcgtca agaccaatcc gtccgacggc acgatccagc tgcagggcac
     1081 cctgggcagc atcaccgcca aaccgcaggt cgggaacaac ggcatcgggc tgcaggtcgt
     1141 caacctcaac gggctcggct tcacgctgcc cagcgagtcg gtgcagcccg cgttggacgc
     1201 gtttgccaac gagctgacca acagctaccc gctcggcatc catgccgaca gcgtgcaggt
     1261 cacggatagc ggtgtggtgg gccacttttc gacgcgcaac gcatccatcc cgcagggcaa
     1321 cccctgcttc gcgaatctgt aagccgctag cccaacccac ccgcagactg gccgaaagga
     1381 aagaaccccc agcgcccggc gagatgtcag gatccgccga tgccgatgcg gacgattccc
     1441 cacggatgcg ggtgcgcgaa ctcgaacctc gccgtgcagc ggataccaac acggtcgctg
     1501 gagaagtacg cggaggcgtc ggcgaccacg gacgggttat cgcggatgac cacgaacgac
     1561 ttggcctgcg ggatggccca aatgtggccg gtgttgacgt aagggctcca gaacacaggc
     1621 acacccaaca cgttagggct ggtgacgttt ccgtgggtca cgaatcgggc actgtcctgc
     1681 ggattccact gtcatgcagg ttgtctgggc gcgccgccta cgggcgcgcc cctcgcgcgt
     1741 caagggcgct tcgcgtcgct tcgcgatggc cttcggccac cccttgaccc gcgagcctct
     1801 gcgcgccctg atggcaggcg aaagggcagg tcagagacct gcccggcgca ttcgcgcggc
     1861 ccagacggaa ggtctcgaga gggccaggat ccgacccacg gaaacgtcac aagatcccaa
     1921 cacgctacgc cgggtggggc ttgtcgggtc gacaccaagc agcggggcgt tgaagccgga
     1981 acctctaacg gaggacgacg ggcttgacgg gctcgattca cttcgtcgtt ctatggatcg
     2041 gaaaaatcga caacgcgcca ccgaacttcc gggcgcgccg actggaacct attcgagccg
     2101 ctgatcaaga acagactgtg gccgaatccg agatacaact ggcgttccgg ttggtcctca
     2161 agcggctaga tgcctcgctg gctggcgacg tcgcgcccgc tcggggagcg gcagcagatc
     2221 ttcaccttgc cggacacgat gacgtagagc cggccgccgg gttcgccttc ggcgaaaacg
     2281 gtgcggccac gggggaagtc agctcgctgg ggccgccggg ccagcggcct ggccgcgctg
     2341 tgttcgacgc ctcgaaaggt cgccgcccaa cggagcagcg cgggcattgt caagttgagc
     2401 gaagtgggcg cggtcgccaa tcacatcagc ctcatgcgtc acatatgacc tgggggttca
     2461 cgagaacgca tcgcctcaaa tcctggtctg attgctcaac ttgacaatgc catcgtcgtg
     2521 ccgtcgtcga tttgtgtggt cacggtgtta ctcctcgacc ggttcatggg catcggcggc
     2581 gagagcgcgc tgttcttcgt cggcgtgcta gtggtcgcgc tgctgggcgg cgtcgctccc
     2641 gcggcgctgt cggcggtgct gtcggggctg ctgctgaact acttcctgac cgagccgcga
     2701 tacacgttca cgatcgccga acccgactcc gccgtcaccg agttggtgct gcttgtcgtc
     2761 gcagtggcgg tggctgccct ggtggacaac gccgccaagc gcacccgcga ggcgagacgg
     2821 gcgtcgcagg aggccgagct tctggccttg ttcgccgggt cggtgctgcg cggcgcggac
     2881 ttggacacgt tgctggaacg ggtgcgtgag acctattcgc aacaagcagt aagcatgctg
     2941 cgcgtccggg aagaccccga tgagggtgag atcgtcgcgt gcgtcggcaa ggatccctgc
     3001 accgacgtcg actcggccga caccgcgatt gaggtcggcg acgatgagtt ctggatgctg
     3061 atggccggcc gcaagctacc tgccagcgac ctgcgggtgc tcactgccgt agcgaagcag
     3121 gcggccggct tgatcaagca gcgcgagctc gccgaggaag ccagcaaggc ggaggcgatc
     3181 gcgcaggccg acgagctgcg ccggtcgctg ctgtcggcag tcagccacga cctgcggaca
     3241 ccgctggccg ctgccaaggc agcggtctcg agcctgcgca gcgccgacat cgcgttctcc
     3301 cccgaggaca ccgccgaact gctcgcaacc atcgaggagt cgatcgacca gctgaccgca
     3361 ctggtgggca acctgctcga ctcgtcgcgg ctggcggccg gcgtcgtgcg cccagaactg
     3421 cgccgggtat atctcgagga agtcgtcaac cgggctctga tgggaatcag caaaggcaac
     3481 accggattcc gaagcgggct tgaccgcgtc aaggtagaag tcgatgacgc ggtcgcgatg
     3541 gccgactccg gcctgctgga gcgtgtgctg gcgaacctgg tcgacaacgc gctgcgctac
     3601 gcgcccgacg gcgtcgtccg ggtctacgcg ggacaagtcg gcgaccgggt cttgatcaac
     3661 gtcgccgacg agggcccggg cgttccgcgc gggatggaag accagctctt cgagccgttc
     3721 cagcggatgg gcgaccgcga caataggttc ggcgtgggcc ttggtctctc agtggcaaag
     3781 ggtttcgtgg aggcgatggg cggcaacatc tccgcgaccg atactcccgg cggtgggttg
     3841 acggtcgtgg tggacctggc agcgccggcg ggggaccggc cgtgacgcgt gtcctggtga
     3901 tcgacgacga gccgcagatt ctgcgggcgt tgcgcatcaa cctgtccgtc cgcggttacg
     3961 aggtgatcac cgcggctacc ggcgcggccg ccttgcgcgt ggccgccgag caccgccccg
     4021 acgtgattgt gctcgacctc gggctgcccg acatgtcggg catcgaggtg ctcgagggtc
     4081 tgcgcgggtg gctgtccgcg ccggtgatcg tgctgtcggc gcgcatcgac tccgccgaca
     4141 aggtcgaagc cctcgacgcc ggtgccga
//
LOCUS       DASWBF010000356         8981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_362384,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000356 DASWBF010000000
VERSION     DASWBF010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362384"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VF874_13125"
     CDS             <1..758
                     /locus_tag="VF874_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176967.1"
                     /translation="EAAPPAGVVEAPLVPAAAAPLVPAAAAVPGAPAAAATPPAAVVE
                     AVPPAAVPPAAVAAPGTSAAAATPPAEAAAATPPAAVVEVAPPASALEAILQEMHDAA
                     PTLATLPVTAAAAAPGAIQELAAPPAALPAVGAPAAAVAPEAGVVAAAPAAAAGLPFI
                     PLAGVAPPASGVEALLLPAAATVPAAPGAVSPAPQLSLSTLANLPAFPQLLPQGLSMP
                     HDLICEGTAWPASVTPFPLVPGLSGLPAGRPIG"
     gene            complement(778..2559)
                     /locus_tag="VF874_13130"
     CDS             complement(778..2559)
                     /locus_tag="VF874_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9176968.1"
                     /translation="MSDADEPSRAGTQFGPYRLIRLLGRGGMGEVYEAEDTVRERKVA
                     LKLMGTAFSQDPVFRKRMQREARTAGRLQEPHVVPIHDFGEIDGQLFVDMRLIEGTDL
                     GSVLNRFGPMAPPRAVAIIAQIASALDAAHTAGVMHRDVKPANVLLTSDDFSYLVDFG
                     IASATTDEKLTQMGSTIGTWIYMAPERFSNRDVSHRADIYSLTCVLFECLTGSAPYKG
                     KGPSVMTAHITQPIPRPSAMRPGIPAALDEVIARGMAKDPAGRYPTAGDLALAAHEAL
                     SAPDRERASDILRRSKVATLPQPRRVGEQVPRQRSSTPPPYAGSAPASGPLPRTTPPP
                     AAPTWGAQSWPSQPQTAWATPQTAAPQQFWGQPPSAPPARNRKPWLLAGAAALLAVVL
                     VVVLIIWLLPDSSPPANTVKLQVLDDGVSVGAADAPTTIDVFNEPICPKCADFIRSYS
                     TDLQTAIDDKKITVRYHLLNDASASRDYSTRALAASLCVASTDDPKLYTDFYAGLFAN
                     DFQPEENGSTDPSDSDLADLAESLGASNAVKICITSGQNLSAAKTKASNASHTLESLM
                     SEPTTPQVFNGTTQIDTSDSSWLDNLY"
     gene            complement(2640..3209)
                     /locus_tag="VF874_13135"
     CDS             complement(2640..3209)
                     /locus_tag="VF874_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9176969.1"
                     /translation="MPTADPTPHRLFQQAWPEGEYRMFQLGFVVDDLLAAADRWVRVF
                     GVGPFHVMPRVSSSCRYRGTDSYVDIHIAVAQAGPVQIELIQDFTDGPSVFRDVHGLH
                     QVSTLTRDYDGKKAHYERMGYEPVCELTSPGQRVAFYDTLEDFGFFTEVVEEKPSFQA
                     NLSNISRTCADWDGADPIRILTRDGYRTP"
     gene            complement(3196..4080)
                     /locus_tag="VF874_13140"
     CDS             complement(3196..4080)
                     /locus_tag="VF874_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX9176970.1"
                     /translation="MTDIDLERAVLEALDGLLAALELKPLGEGHFRAHSEPDRFGRVF
                     GGQMVAQAMYAACATVPDKPPHSLHAYFVQAGVPERPLDLDVDRVRDGRSMSARRVTV
                     AQDGEPLLIAMVSFHDNPTEPEFAEPAPPAPGPDELPRLQDWAEKAPPQLLSRARMWV
                     DRPPPVEMRIGEPTYFLGGSAASGPRTHWMRLPRDVGADPKLHSVLLAYASDYLLLDM
                     AFRSHPERVTVASFAGFSLDHALWLHRPVRFDRWHRHTQELVAVSGDRGLVRGMIHDI
                     DGHLVANTTQEVLVRANR"
     gene            complement(4077..5267)
                     /locus_tag="VF874_13145"
     CDS             complement(4077..5267)
                     /locus_tag="VF874_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176971.1"
                     /translation="MTASLPSWADQMKALENVADDLITKWRPDGATEAEIQDMNKLAL
                     SMLASGYLCRVYTDIRRPVFMPMWNYAFNQGGPSPDYVYSTVEIDPQGVYRISGYRGT
                     TRFVELTQQAIDILNLQAMDGKAPYAFTNDLDELSLGEDGSFSVILSAQRPEGYSGDW
                     WQLHDRTCRILMRRCSCDWNREIDARVAIERLDANGADMTPEEIARRFSDLSPWIEGV
                     IEFDMRLIRYYREHHGVNTLLRSGKIDEMGGLPKQAYYDGIHEIDDSEALIIETELPQ
                     RCRYWQALVGDDRFCTVDWVNRQSSLNDAQARIDGDGKFRAVISRLDPAVPNWLDKGD
                     YPWGIIQMRWNQATDYPDPTIKKVPFADIRDHLPADTPVVTPEERKEQLRIRREGAQL
                     RRIW"
     gene            complement(5295..6440)
                     /locus_tag="VF874_13150"
     CDS             complement(5295..6440)
                     /locus_tag="VF874_13150"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9176972.1"
                     /translation="MTRRYPTSDQLMADAVQESGLSDFGPGDFRDGLDMLLDSLERDG
                     DLSPAADAGVIADFRRRLVNRLEVEAWYRENPEIEQVPVLGPVDINGLPRTGTTALAN
                     MMSLDPQFRCLRGWEQSKPCPPPTIDTEATDPRRIAAAQFNEQLPPEAKALHLYDLDA
                     TMEDTELLGMAFHGQGYTLPVYSYHAWWRHADLTATYRYHRRVVKLLASQRPPNLWLF
                     KAPHHMFHLEAIVAAYPQVRFVMTHRDPAKVVPSYVSTVNMFFPPPAGERDMHRLGRE
                     VSEHLRDGMRNTIAARARLGEDRFIDVHHRDLVADPVRTLRRVYAFVGVQWPAAMEQA
                     LADWQRRNRPGAHGVHRYTAEQFGLDPARIRDDYGFYLDRFDVTVES"
     gene            complement(6437..7378)
                     /locus_tag="VF874_13155"
     CDS             complement(6437..7378)
                     /locus_tag="VF874_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9176973.1"
                     /translation="MTESTVLHDEKILITGPAGRIAFGIAQSLVGDNEVWGVARFSDA
                     ASREKVESLGVTTQAVDIADGRFGDLPGDFTYLLHVAADFSADDYERALRVNAEGTGF
                     VLEHCRTAKAALVMSTVTIYKPHPDPWHAFREDDPPGDAMLPQQAPYSISKIAEEAVA
                     RYCARSLNLPTTIARMCSAYGERGGLPVWHMHALAAGEPVRTRWDPMPYSPIHDDDIC
                     AQLKPLLDVAGVPATIVNWGGDEPVSVQQWSAYLGELLGVEPEVLVEEVPGASIGSVA
                     DHTKRAAITGPCRVHWRDGLRRVAEQLYPDRVRSTTS"
     gene            complement(7375..8337)
                     /locus_tag="VF874_13160"
     CDS             complement(7375..8337)
                     /locus_tag="VF874_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9176974.1"
                     /translation="MRPIRFATGGGRVADRERLVAAARRAEEVGYSTFSMADHFGLPF
                     APLIALQAVADATTTLRITQTVLDHDFRRPAVLAKELATLDVLSGGRTEVGLGAGWMR
                     AEYEQAGIPFDPAPVRIARLEEVVIVLKGLFGEQPFSFSGKHVTITGLDGTPKPLQKP
                     HPPILVGGGGPKLLAVAARQADIIQVMPRIQRPGQPIDVREFTAATYRCKIDHIKDVA
                     GERFREIELGTQLLLAAVTDDPDRVIDEFIAGFGAVYGRPGAQLALSRDELRASPLVA
                     VGALDEVCDKLLATRDEYGISYFACPMQTNPTSLAPIIDRLAGR"
     gene            complement(8337..8663)
                     /locus_tag="VF874_13165"
     CDS             complement(8337..8663)
                     /locus_tag="VF874_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9176975.1"
                     /translation="MGRVDGKVALVTGARHIRLNVVHPTNCDTEMLHNEPTYQSFRPD
                     LDHPSREDAEPAFHVMQAMPLPYIDPLDISNAVLFLASDEARFVTGMQLRVDAGGYLK
                     IYDYHV"
     gene            complement(8747..>8981)
                     /locus_tag="VF874_13170"
     CDS             complement(8747..>8981)
                     /locus_tag="VF874_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176976.1"
                     /translation="ELHREIVERWQPPGADVDSSVIDDQTYVTMNFISGVMMSLAAGD
                     RTIRSPAHLDRLLSNIITGITTAHQHNEAVSRP"
BASE COUNT         1401 a         2885 c         3126 g         1569 t
ORIGIN      
        1 tcgaggccgc gccgccagcc ggtgttgtcg aggcccccct tgtgcccgct gcagcggccc
       61 cccttgtgcc cgctgccgcg gctgttccgg gcgctcccgc tgctgccgcg accccgccgg
      121 ctgcggtggt agaagctgtg ccgcctgcgg ctgtcccgcc ggctgctgtc gctgcgccgg
      181 gaacttctgc tgctgcggcg accccgccgg ctgaggccgc tgcggcgact ccgccggccg
      241 ccgtggttga ggttgcgccg cccgccagcg cgctggaggc gatcctccag gagatgcacg
      301 acgccgcccc gactctggcc acccttccgg tgaccgcagc agccgccgcg ccgggggcca
      361 tccaggagct ggccgcgccg ccagccgcgc tgcccgcagt gggcgcgccg gcagcagcgg
      421 ttgcgccgga ggccggggtc gtggcagccg cgcctgcggc ggcagccggt cttccgttca
      481 ttccgctcgc cggggtcgcc ccaccggcca gcggcgtgga agccctacta ctgccagctg
      541 ccgcaacggt tcccgctgcg ccgggtgcgg tgagccctgc gccccagctc agcttgtcaa
      601 cgttggcgaa ccttccggcg tttccccagt tgctgcctca ggggctgtcg atgccgcacg
      661 acctcatctg tgagggcacc gcgtggccgg cgtccgtgac gcccttcccg cttgtccccg
      721 gcttgtccgg actcccggca ggacgaccga tcggataacc aacggccgca acgcttgtca
      781 gtagagattg tcgagccagg acgaatccga cgtgtcgatc tgtgtcgtgc cgttgaagac
      841 ctgcggtgtg gtgggctccg acatgaggct ctccaacgtg tgagaagcat tgctggcctt
      901 ggtttttgcc gcactgaggt tctgcccgga cgtaatgcag atcttgacgg cattggacgc
      961 acccagcgat tcagcgagat cagccaagtc agagtcggag ggatcggtgg agccgttctc
     1021 ctcgggctgg aagtcgttcg cgaacaagcc ggcgtagaag tcggtgtaca gcttcgggtc
     1081 gtcagtcgag gcgacgcaca acgacgctgc cagcgcacgg gtggagtagt cacggctggc
     1141 ggaggcgtcg ttgaggaggt ggtagcgcac ggtgatcttc ttgtcgtcga tcgcggtctg
     1201 caggtcggtc gagtaggacc ggatgaaatc ggcgcacttc gggcagatcg gctcgttgaa
     1261 gacgtcgatc gtcgtcggcg catcggcggc accgacggac acgccgtcgt cgagcacctg
     1321 cagcttcacc gtgttggccg gaggcgacga atcgggcaac agccagatga tcaggaccac
     1381 gacgagcacc accgcgagca acgccgcagc gccggccagc agccacggct tgcggttgcg
     1441 cgctggcggc gcagatggcg gctggcccca gaactgttgt ggcgcagcgg tttggggcgt
     1501 cgcccacgcg gtctgaggtt gactcggcca agactgcgca ccccacgtcg gggcggcggg
     1561 cggtggtgtg gtgcgtggca gcgggcccga cgccggggca ctgccggcgt acggtggcgg
     1621 cgtcgacgaa cgttggcgag gcacctgttc gccgactcga cgcggctgcg gcagcgtcgc
     1681 caccttgctg cgccgcagga tgtcggacgc gcgttcccgg tcgggtgcgc tgagcgcctc
     1741 gtgcgcggcc agcgcaagat cgccggcggt ggggtagcgg cccgcgggat ctttcgccat
     1801 gccgcgggca atcacctcgt ccaaagctgc cgggatcccc ggccgcatcg cgctcggccg
     1861 gggaatcggc tgcgtgatat gcgccgtcat cacgctgggt cccttgccct tgtacggcgc
     1921 ggagccggtc aggcactcga acaacacaca ggtcagcgag tagatgtccg cgcgatgcga
     1981 cacgtcgcgg ttgctgaacc gctcgggcgc catgtagatc caggtgccga tcgtgctgcc
     2041 catctgggtg agcttctcgt ccgtggtggc gctcgcaatg ccgaagtcga cgaggtagga
     2101 gaagtcgtcg ctcgtcaaca ggacattggc cggtttgacg tcacggtgca tcacaccggc
     2161 ggtgtgtgcg gcgtcgagcg ccgaggcgat ctgggcgatg atcgcgactg cccgcggcgg
     2221 cgccatcggg ccgaaccggt tcaacacgct gcccaggtcg gtgccctcga tcaggcgcat
     2281 gtccacgaac agctgtccgt cgatttcgcc gaagtcgtgg atcggcacga catgcggttc
     2341 ctgcaagcgg cccgcggtgc gcgcctcgcg ctgcatccgc ttgcggaaca ccggatcctg
     2401 cgagaacgcc gtgcccatca acttcagcgc gaccttgcgc tcgcggaccg tgtcctcggc
     2461 ctcatacacc tcacccatgc cgccgcggcc cagcagccgg atgagccggt aggggccgaa
     2521 ttgcgtgccc gcgcgcgacg gttcgtcggc gtcgctcatc gtcgatttct ccgcatttcg
     2581 acgacttcgc tcacactgca aatcatcgca gaccgggccg cacatgggcc gcactcgggc
     2641 tacggagtgc ggtagccgtc gcgggtgagg atgcggatcg ggtcggcgcc gtcccagtcg
     2701 gcgcacgtcc gcgagatatt ggacaggttt gcctggaagc tgggtttttc ttcgacgact
     2761 tcggtgaaga agccgaagtc ctcgagcgtg tcgtagaagg cgacgcgctg gcccggggag
     2821 gtgagctcgc agaccggttc gtatcccatg cgttcgtaat gcgccttctt gccgtcgtag
     2881 tcgcgggtca gagtggacac ctgatgtaag ccgtggacat cgcgaaacac gctcgggccg
     2941 tcggtgaagt cctggatcag ctcaatctgc accggaccgg cctgagccac cgcgatgtgg
     3001 atgtccacgt acgagtcggt gccgcgatag cggcaggaac tactcacccg cggcatcacg
     3061 tgaaacggcc cgacgccgaa cacgcgtacc cagcggtccg cggcggcgag caggtcatcg
     3121 acgacgaagc cgagctggaa catgcggtac tcgccctcgg gccacgcctg ctggaacagg
     3181 cggtggggag tgggatcagc ggttggcacg gacgagcacc tcctgagtcg tgttggccac
     3241 gaggtgcccg tcgatgtcgt ggatcatccc gcgcaccagg ccgcggtcac cggagacggc
     3301 caccagctcc tgtgtatgcc gatgccatcg gtcgaagcgc accggtcggt gcagccacag
     3361 cgcgtgatcg aggctgaatc cggcgaacga cgcgacggtg acgcgctcgg ggtgggagcg
     3421 aaacgccatg tccagcaaca gatagtcgct ggcgtaggcg agcaggacgc tgtgcagctt
     3481 agggtcggcg ccgacgtcac gcggcagtcg catccaatgg gtgcgggggc cgctggcagc
     3541 cgacccgccc aagaaatacg tgggctcgcc gatgcgcatc tccaccggcg gcggccggtc
     3601 gacccacatc cgcgcgcgcg aaagcagctg cggcggcgcc ttttccgccc agtcctgaag
     3661 gcgcggcagc tcgtcgggtc ctggtgccgg tggggccggt tcggcgaatt caggctccgt
     3721 cgggttgtcg tggaaggaga ccatcgcgat cagcaacggc tctccgtcct gcgccaccgt
     3781 cacgcgtcga gcagacatcg accgcccgtc ccgcaccctg tcgacgtcca gatcaagcgg
     3841 gcgctcggga acgccggcct gcacgaagta ggcatgcagg gaatgcggtg gtttgtcggg
     3901 cacggtggcg cacgcggcat acatggcttg agcgaccatc tggccgccga agacgcgccc
     3961 gaagcggtcg ggctcgctgt gggcgcgaaa atgtccttcg cccaacggct ttagctcaag
     4021 ggccgctaac aagccatcga gcgcctcgag gaccgcgcgc tccagatcaa tgtcagtcac
     4081 cagatccgcc ggagttgcgc gccttcgcgg cggatgcgga gctgttcctt gcgttcttcc
     4141 ggcgtgacga ccggtgtgtc ggccggcaga tggtcgcgga tgtcggcgaa gggaaccttc
     4201 ttgatggtcg ggtcggggta gtcggtggcc tggttccagc gcatctggat gatgccccag
     4261 ggatagtcgc ccttgtcgag ccagttgggc actgccggat cgagtcgact gatcaccgcg
     4321 cggaacttcc cgtcgccgtc gatacgcgct tgggcgtcgt tcaggctgga ctgccggttg
     4381 acccagtcga ccgtgcaaaa ccggtcgtcg ccgacgagtg cttgccagta gcggcaccgc
     4441 tgcggcaact cggtttcgat gatcagcgcc tcgctgtcgt cgatctcgtg gatcccgtcg
     4501 tagtaggcct gcttcggcag tccgcccatc tcgtcgatct tgccggaccg cagcaacgtg
     4561 ttgacgccgt ggtgctcgcg gtagtaccgg atgaggcgca tgtcgaactc gatcaccccc
     4621 tcgatccacg gggacagatc agagaagcgg cgtgcgatct cctctggggt catgtcggcg
     4681 ccgttggcgt cgagacgctc gattgccact ctcgcgtcga tctcccggtt ccagtcacat
     4741 gagcagcggc gcatcaggat tcggcaggtc cggtcgtgca gctgccacca gtcgccgctg
     4801 tagccttcgg gccgctgggc ggacaggatc acgctgaaag aaccgtcctc acccaggctg
     4861 agttcgtcga ggtcgttggt gaaggcgtag ggcgccttgc cgtccatcgc ctgcaggttg
     4921 aggatgtcga tggcctgctg cgtcagttcg acgaatcgcg tggtgccgcg ataccccgaa
     4981 atgcggtaca cgccttgcgg atcgatctcg acggtcgagt agacgtagtc ggggctgggg
     5041 ccaccctggt tgaatgcgta gttccacatc ggcatgaaca cgggccggcg gatgtcggtg
     5101 tagacccggc acaggtaacc gctggccagc atcgacaacg cgagcttgtt catgtcttgg
     5161 atctcggcct cggtggcgcc gtccgggcgc cacttggtga tcaggtcgtc ggcgacgttc
     5221 tccaacgcct tcatctggtc ggcccagctc ggcaacgatg ccgtcatcgt cgttcccttt
     5281 ctcgttgcgg cggttcagga ttcgacggtg acgtcgaagc ggtcgaggta gaagccgtag
     5341 tcgtcgcgga tgcgcgccgg atcaagtccg aactgctccg cggtgtagcg gtgcaccccg
     5401 tgggcgcccg gtcggttgcg tcgctgccag tcggcgagcg cctgctccat cgcggcgggc
     5461 cactgcacgc cgacgaacgc gtagacgcgg cgcagcgtgc ggaccggatc ggcgaccaag
     5521 tcccggtggt ggacgtcgat gaaccgatcc tcgccaagcc gtgcccgcgc ggcgatggtg
     5581 ttgcgcatcc cgtcgcgcag atgttcggag acctccctgc cgaggcggtg catgtcgcgc
     5641 tcgccggcgg gtggagggaa gaacatgttc acggtgctga cgtaggacgg cacgaccttg
     5701 gcggggtcac gatgcgtcat cacgaatcgg acctgcgggt aggccgccac gatcgcctcc
     5761 agatggaaca tgtggtgcgg cgccttgaac agccacaggt tcggaggacg ttgcgaggca
     5821 agaagtttca cgacgcgccg atggtaccga taggtggctg tgagatcggc gtgacgccac
     5881 caggcgtggt agctgtagac gggcagcgtg tagccctggc cgtgaaacgc catcccgagc
     5941 agctccgtgt cctccatggt ggcgtcgagg tcgtacaggt gcaacgcctt ggcctccggc
     6001 ggtagctgct cgttgaactg ggctgccgcg atccggcggg ggtccgtggc ttcggtgtcg
     6061 atcgtgggtg gcgggcaggg cttgctctgt tcccacccac gcagacaacg gaattgcgga
     6121 tccagcgaca tcatgttggc cagggcggtg gtgcccgtgc gcggcagccc gttgatgtcg
     6181 accggaccca gcaccggaac ctgctcgatc tcgggattct cgcggtacca cgcctcgact
     6241 tcgagacggt tgaccagccg tcgccgaaag tcggcgatca cgccggcgtc cgccgcggga
     6301 ctcaggtcgc cgtcgcgttc caggctgtcg agaagcatgt cgaggccgtc gcggaagtcg
     6361 ccggggccga aatccgagag cccgctttcc tgcacggcgt cggccatcag ctggtcgctc
     6421 gtggggtatc ggcgagtcat gacgtggtgc tccgaacgcg gtccgggtaa agctgttcgg
     6481 ccacccggcg cagcccgtct cgccagtgaa cgcggcacgg gccggtgatc gcggcgcgct
     6541 tagtgtggtc ggcaaccgac ccgatcgagg caccgggaac ctcctcgacg agcacttccg
     6601 gttccacgcc gagcagctcg ccgagatagg ccgaccactg ctggacgctg acgggctcgt
     6661 cgccgcccca gttgacgatc gtggccggca cgccggcgac gtcgagcagt ggcttgagct
     6721 gtgcacagat gtcgtcgtcg tgaatcgggc tgtacggcat aggatcccag cgcgtccgca
     6781 cgggctcgcc cgccgcgagt gcgtgcatgt gccagactgg caaaccgccg cgctcgccgt
     6841 aggcggagca catccgggcg atggtcgtcg ggaggttcaa cgaccgcgcg cagtaccgcg
     6901 cgacggcctc ctcggcgatc ttggagatcg agtacggcgc ctgctggggc agcatcgcgt
     6961 cgccgggcgg gtcgtcctca cgaaaggcgt gccaggggtc gggatgcggc ttgtagatcg
     7021 tgaccgtcga catcaccagc gccgccttcg cggtgcggca gtgctcgagc acgaacccgg
     7081 tgccttcggc gttcacccgt agcgcgcgct cgtagtcgtc tgcgctgaaa tcggcggcga
     7141 cgtgcagcag ataggtgaaa tcgcctggaa gatcgccgaa tcggccgtcg gcgatgtcga
     7201 cagcctgggt ggtcactccg agcgactcga ccttctcgcg cgacgccgcg tcgctgaacc
     7261 gcgcgacacc ccacacctcg ttgtcgccga cgagtgactg ggcgatgccg aacgcgatcc
     7321 gcccggccgg tccggtgatc agaatcttct cgtcgtgcag cacggtggac tccgtcacct
     7381 cccagccaac cggtcgatga tgggcgccaa cgacgtcgga ttggtctgca tcggacaggc
     7441 gaagtagctg atgccgtatt cgtcacgggt ggcgagcagc ttgtcgcaca cctcgtcgag
     7501 cgcgccgacc gccacgagcg gagacgcgcg cagttcgtct ctgctcaggg cgagttgtgc
     7561 gcctggccgg ccgtagaccg caccgaagcc ggcgatgaac tcgtcgatga cccgatcggg
     7621 atcgtcggtg accgcggcaa gcagcagctg ggtgccgagt tcgatctctc ggaagcgctc
     7681 tccggcaacg tctttgatgt ggtcgatctt gcaccggtag gttgccgcgg tgaactcgcg
     7741 cacgtcgatc ggctggccgg gacgttggat gcgtggcatc acctggatga tgtcggcctg
     7801 gcgagcggcg acggccagaa gcttcgggcc gcccccgccg acgaggatcg gcggatgcgg
     7861 cttctgcagc ggcttgggcg tgccgtccaa cccggtgatg gtcacgtgct taccggagaa
     7921 gctgaacggc tgttcgccga aaagcccttt cagcacgatg acgacttcct cgaggcgggc
     7981 gattcggacc ggagcggggt cgaacggaat gccggcctgt tcgtattccg cgcgcatcca
     8041 gcccgcgccg agaccgacct cggtgcgtcc gccggacaac acgtcgagtg tggccagttc
     8101 cttcgcgagc actgcgggcc ggcggaagtc gtggtcgaga acggtctgag tgatccgcag
     8161 cgtcgtcgtc gcgtcggcga cagcctgcag ggcgatcagc ggagcgaacg gcagtccgaa
     8221 atgatcagcc atcgaaaacg tggagtagcc cacctcctcg gcccgtcgtg cagcggcgac
     8281 aagccgctca cggtcggcca cgcgaccgcc tcccgtagcg aaccggattg gccgcatcag
     8341 acgtggtagt cgtagatctt gaggtaacct ccggcgtcga cccgcagttg catgccggtg
     8401 acgaatcggg cctcatcgga cgccaagaac agtacggcgt tgctgatgtc gagtgggtcg
     8461 atgtagggca gtggcatcgc ctgcatcacg tggaacgccg gctcggcatc ttcgcgcgac
     8521 ggatggtcga ggtccggccg gaaggactgg taggtgggct cgttgtgaag catttccgtg
     8581 tcgcagttgg tcggatgaac gacgttgagc cggatgtggc gcgctccggt gacgagagcc
     8641 accttgccgt ctacccggcc catctgtgcc tccgtactgc gactccgtca ggactctaat
     8701 atattagagg ctaggctgtg ggtgttgtgt ccgggtctcg actcgcctac ggtcgtgaga
     8761 ccgcctcgtt gtgctgatgc gcggtcgtga tgccggtgat gatgttcgac agaagccggt
     8821 cgaggtgcgc tggactgcgg atcgtgcggt cgcccgcggc caaactcatc atgacgccgc
     8881 tgatgaaatt cattgtgaca taggtctgat cgtcgatcac cgaggaatcg acatcggccc
     8941 cgggcggctg ccaccgctcg acgatctcgc gatgcaactc g
//
LOCUS       DASWBF010000357         9736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1149108,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000357 DASWBF010000000
VERSION     DASWBF010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149108"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VF874_13175"
     CDS             <1..1345
                     /locus_tag="VF874_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX9176977.1"
                     /translation="ESAPELRTPWPREALNDLLVLLSAGPTAVTTIEALDRTGLWGRL
                     FPDWGAVRDLPPRDIVHIWTVDRHLTETVSRASAFTTRVSRPDLLVLGALMHDIGKGR
                     GGDHSVIGAELATQVGTRLGLWPSDVDILSKIVRHHLLLASTATRRDLQDPETINMVV
                     GAVDGDPLLLELLHTLAEADSLATGPGVWGDWKALLIGDLVRRCRIAMAGEPLPQADP
                     IDEQHLSLAADGKVHVVLTPGDGAHIYRVTMVAPDRRGLLSKAAGVLALNSLSVHSAS
                     VNSHEGSAINTFVVSPHFGSPPAAELLRQQLIQALDDDLDVIALLDQRHREATQHGSG
                     RAGEVLAAVPANQVPAPPRILWRDGSAPGQLLVEIRSTDRAGLLALLTRVFEKAGIDI
                     TWAKVTTLGSSVDDVFCVVPPALGTSDVERLRATLERDLLSVLPAPPQKPAENAS"
     gene            1356..2912
                     /gene="ffh"
                     /locus_tag="VF874_13180"
     CDS             1356..2912
                     /gene="ffh"
                     /locus_tag="VF874_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX9176978.1"
                     /translation="MFETLSDRLTGALQNLRGKGRLSDADIDATTREIRLALLEADVS
                     LPVVRAFVARIKERAKGAEVSGALNPAQQVVKIVNDELIGILGGQTRKLAFAKTPPTV
                     VMLAGLQGSGKTTLAGKLAKWLKGQGHTPLLIAADLQRPGAVNQLKIVGERAGVQVFA
                     PHPGTSPEGSADVGPGDPVAVSAAGLSEAKAKHFDVVIVDTAGRLGIDEELMTQAAAI
                     RDAVGPDEVLFVLDAMVGQDAVATADAFREGVGFTGVVLTKLDGDARGGAALSVREVT
                     GVPILFASTGEKLEDFDVFHPDRMASRILGMGDVLSLIEQAEQVFDAEQAEATAAKIG
                     SGDLTLEDFLEQMLTIRKMGPIGNLLGMLPGAGQMKEALAAVDDSHLDRVQAIIRGMT
                     PAERADPKIINASRRLRIANGSGVTVSEVNQLVDRFFEARKMMSQMAGQMGLPGFGRK
                     SSRKAKGGKGKKGKKGARGPTPPKVRGPLGAGMPGGAFPDLSQMPPGLDELPPALADF
                     DLSKLKFPKN"
     gene            2855..4042
                     /locus_tag="VF874_13185"
     CDS             2855..4042
                     /locus_tag="VF874_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9176979.1"
                     /translation="MAACAGGFRFVEAEVPEELAVPLHVRGRGLPSGEPVEWWIIDER
                     SREEHGYSGTLSAEPVSGADTVFDGGWILPGLVDAHCHVGVGADGPAADLDEAIAQAE
                     TERNAGALLLRDCGSPIDTRSLDDHDDLPRIIRAGRHLARPKRYTPGLPIDVEDESQL
                     PEAVAAQARRGDGWVKLVGDWIDREVGDLAPLWSDHVLKAAIDTAHAHGARVTAHVFG
                     EDALPGLVTAGIDCIEHGTGITDDVIDLMVERGTALVPTLINIDNFPGIADRAGNYPV
                     YAAHMRDLHTTSRHRVAAAREAGVPVYAGTDAGSMIVHGRIADEVDALKGVGMSPTDA
                     LGAACWDARRWLGRPGLEHGASADLICFADDPRTGAAVLNRPDLVILRGRVFAGYSDP
                     TLA"
     gene            complement(4018..4890)
                     /locus_tag="VF874_13190"
     CDS             complement(4018..4890)
                     /locus_tag="VF874_13190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX9176980.1"
                     /translation="MRKLVAPLAAIVAACGLSAPVAFADPAVQPAGAVAAPEGPAPAW
                     LVADMDTGQILAARDAYVPHAPASTNKVLLALTALDEVGLGNTVTATVADTQVECNCV
                     GIKPGRTYTARQLLDAILLVSGNDAANTLADMLGGFDVAVAKMNAKAEQIGAYDTHAA
                     TPSGLDGPGMDFRTTPHDLAVIFRAALANPVFAMITSQPSALFPTDAGETPIVNQDEL
                     LRRYPGALGGKTGFTNIARKTFVGAAERNGRRLIVVQMYGLEKPGGPTYWDQAISLLD
                     WGFAQSPQASVGSL"
     gene            4937..5356
                     /locus_tag="VF874_13195"
     CDS             4937..5356
                     /locus_tag="VF874_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9176981.1"
                     /translation="MLSLEEISDRLEIQQLLIDYSTAIDQRRFDDLDRVFTPDAYIDY
                     RVMGGIDGTYPAVKAWLAQVLPNFPVYAHMLGNFDVRISGDTASSRTICFNPMVLGGD
                     SGNVMFCGLWYDDEFVRTPEGWRMTRRVEAKCFDKVL"
     gene            5556..6074
                     /gene="rpsP"
                     /locus_tag="VF874_13200"
     CDS             5556..6074
                     /gene="rpsP"
                     /locus_tag="VF874_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX9176982.1"
                     /translation="MAVKIKLTRLGKIRNPQYRIAVADARTRRDGRAIEVIGRYHPKE
                     EPSLIEIDSDRAQYWLSVGAQPTEPVLKLLKITGDWQKFKGLPGTEGTLKVAPPKPSK
                     LELFNAALAEAEGGPTAEATTPKKKAAGAAKAATSDSANAPEKAETADIDTAEATADA
                     PAEAPPAADAES"
     gene            6082..6324
                     /locus_tag="VF874_13205"
     CDS             6082..6324
                     /locus_tag="VF874_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9176983.1"
                     /translation="MSSVVVDAVEHLVRGIVDNPDDVRVDMVTNRRGRTVEVHVHPDD
                     LGKVIGRGGRTATALRTLVAGIGGRGIRVDVVDTDQ"
     gene            6331..6849
                     /gene="rimM"
                     /locus_tag="VF874_13210"
     CDS             6331..6849
                     /gene="rimM"
                     /locus_tag="VF874_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005111677.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX9176984.1"
                     /translation="MELVVGRVVKAHGVTGELVVEVRTDDPDSRFAVGSTLRGKPRHG
                     AAREYTVESVRDHGGRLLVRLAGIDDRDAADAMRGVVFVIDSAELPPTDDPDEFYDHQ
                     LEGLAVRTVDGRDVGTVTEVLHTAAGELLAIRRDDDGESLVPFVTAIVTSVSLADRLI
                     EIDPPEGLLELA"
     gene            6852..7529
                     /gene="trmD"
                     /locus_tag="VF874_13215"
     CDS             6852..7529
                     /gene="trmD"
                     /locus_tag="VF874_13215"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9176985.1"
                     /translation="MRIDVITIFPQYLEPLRESLPGKAIDAGIVDLRVHDLRQWTHDA
                     HHSVDDAPYGGGPGMVMTAPAWGEALDEICSAETLLVVPTPAGVPFKQQDAQRWSSEA
                     RLVFACGRYEGIDQRVIDDAARRMRVEEVSIGDYVLPGGESAALVMIEAVVRLLPQVL
                     GNPESHQQDSYSDGLLEGPAYTRPPSWRGLDVPDVLLSGDHARIAAWRREQGLQRTRE
                     RRPDLLD"
     gene            complement(7526..8317)
                     /locus_tag="VF874_13220"
     CDS             complement(7526..8317)
                     /locus_tag="VF874_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176986.1"
                     /translation="MGAQRPLPDTPPPDTAKVALDGLESRIQQATLQAAASGAEISVL
                     VLDRVTGKTVSNGDDSPFPIASVAKLFIADDLLLQEAKGQTQLSPADRAALEVMLRSS
                     DDNAAENFWNRSGGSEIITRVTERYGLTATTTPYNGRWFNTLSTASDLVRYYDKLLDG
                     SGGLPAQQASIIVSNLAQSTPTGIDGYSQQFGIPDALYAEPVAVKQGWMCCWNGGNWM
                     HESTGVIGWDRRYVMAIGSMQPTDDSMARETITQAVKTMFPGGRI"
     gene            8754..9095
                     /gene="rplS"
                     /locus_tag="VF874_13225"
     CDS             8754..9095
                     /gene="rplS"
                     /locus_tag="VF874_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9176987.1"
                     /translation="MNTLDFVDQTSLRDDIPAFNPGDTVNVHVKVIEGSKERIQVFKG
                     VVIRRQGGGIRETFTVRKESYGVGVERTFPVHSPNIDHIEVVTRGDVRRAKLYYLREL
                     RGKKAKIKERR"
     gene            9114..>9736
                     /gene="lepB"
                     /locus_tag="VF874_13230"
     CDS             9114..>9736
                     /gene="lepB"
                     /locus_tag="VF874_13230"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9176988.1"
                     /translation="MTDKTESADETDAEADSKASTEDGDTPKRKKSVRSVLIEAAIVI
                     VVLVVPFYLVQTFVARPYLIPSESMEPTLHGCLGCTGDRVVVDKVIYRFGSPQPGDVI
                     VFKGPPAWNVGYKSIRSENAPVRWLQNTVSFIGFVPPDENDLVKRVIAVGGQTVECRN
                     ASGLTVDGKPLNQPFLNTKTMGVENLDPAVYRCLGPEFGPVTVPDGRL"
BASE COUNT         1604 a         3239 c         3365 g         1528 t
ORIGIN      
        1 cgagtccgca ccggaattgc gcaccccgtg gccgcgcgag gcgctcaacg acctgctggt
       61 gttgctttcc gcaggcccga ctgccgtgac gacgatcgag gcgctggacc gaacgggctt
      121 gtggggcagg ctgtttccgg attggggcgc ggtgcgcgac cttccgccac gcgacatcgt
      181 ccacatctgg acggtggacc gtcatctcac cgaaaccgtc agcagggcaa gcgctttcac
      241 cacccgggtg tcccgcccgg atctgctggt gctcggtgcg ctgatgcacg acatcggcaa
      301 gggccgcggc ggcgaccaca gcgtgatcgg cgcggagctg gcgacgcagg tcgggacccg
      361 gctcgggctg tggccgtcgg acgtcgacat cctgtccaag atcgtgcgcc accacctgct
      421 gttggccagc acggccacac gccgggacct gcaggacccc gagacgatca acatggtcgt
      481 cggcgcggtg gacggcgacc cgctgctgct cgagttgttg cacacgctgg ccgaagcgga
      541 ctcgctggcc accgggcccg gagtgtgggg cgactggaaa gcgctgctga tcggcgacct
      601 cgtgcggagg tgtcggatag ccatggccgg cgagccgttg ccgcaggcgg atccgattga
      661 cgagcaacac ctttcgctcg ccgccgatgg caaggtacac gtcgtgctaa cgcctggcga
      721 cggcgcccac atctatcgcg tgacgatggt ggcccccgac cggcgcgggc tgctctccaa
      781 ggccgcaggc gtgcttgcgc tgaactcgct gagcgtccat tcggcgtcgg tcaacagcca
      841 cgagggctcg gcgatcaaca cgttcgtcgt ctcgccgcac ttcggctccc ccccggccgc
      901 ggagctgctc cgtcaacaac tcatccaggc actcgacgac gacctggacg tcatcgcgct
      961 gctggaccag cgccaccgcg aagcaaccca gcacggctcg ggccgggccg gcgaggtgct
     1021 cgcggcggtg ccggcgaacc aggtgcccgc gccgccgcgg attctctggc gcgacggcag
     1081 cgcacctggc cagctgctgg tggagatccg ctccaccgac cgggccggcc tgttggcctt
     1141 gctcacacga gtcttcgaga aggcgggaat cgacatcact tgggcgaaag tcaccacgct
     1201 gggttcgtcg gtggacgacg tgttctgcgt cgtgccaccg gcactgggca ccagcgacgt
     1261 ggagcgtctg cgggcgacgc tcgaacgtga cctgctgtcg gtgctgcctg cgccgccgca
     1321 gaagcccgcc gagaacgcca gctaggctgg ccgacgtgtt cgaaaccctg tctgaccggt
     1381 tgaccggtgc tctgcagaac ctgcgcggca agggccggtt atccgacgcc gacatcgacg
     1441 ccaccacgcg cgaaatccgg ctggcgctgc tcgaggccga cgtctcgctg ccggtggtgc
     1501 gtgcattcgt cgcacgcatc aaggagcggg ccaagggcgc agaggtctct ggcgccttga
     1561 acccggcgca gcaagtcgtc aagatcgtca acgacgaact gatcgggatc ctcggcggcc
     1621 agacccggaa gctcgcgttc gccaagacgc cgccgaccgt cgtgatgctg gccgggcttc
     1681 agggctccgg taagacgacg ctcgccggca agctcgcgaa atggcttaag ggacaaggtc
     1741 atacgccgct gctcattgcc gcagatctgc agcgcccggg tgcggtcaac cagctgaaga
     1801 tcgtgggcga gcgcgcgggt gtgcaggttt tcgccccgca cccgggcacg tcaccggaag
     1861 ggtcggccga cgtcggccct ggcgatcccg tcgccgtgtc ggcggccggg ctgtccgagg
     1921 ccaaggccaa gcacttcgac gtggtgatcg tcgacaccgc cggacggctc ggcatcgacg
     1981 aggagctgat gacccaggcc gcggcgatcc gcgacgccgt gggccccgac gaggtgcttt
     2041 tcgtgctcga cgcgatggtc ggccaagacg cggtcgccac cgccgacgcg ttccgcgagg
     2101 gcgtcgggtt caccggtgtg gtgctgacca agctcgacgg cgacgcccgc ggcggcgcgg
     2161 ccctgtcggt ccgcgaggtc accggtgtgc cgatcctgtt cgcgtccacc ggggaaaagc
     2221 tcgaggattt cgatgtcttc caccccgacc ggatggccag ccggatcctc ggcatgggcg
     2281 acgtgctgag cctcatcgag caggccgagc aggtcttcga cgctgagcag gccgaagcga
     2341 cggccgccaa gatcgggagc ggggatctca cactcgagga cttcctcgaa cagatgctga
     2401 cgatccgcaa gatggggccg atcggcaacc tactcggcat gctgccgggg gcgggccaga
     2461 tgaaagaagc cctcgccgcc gtcgacgaca gccacctcga ccgcgtgcag gcgatcatcc
     2521 gcggcatgac accggccgaa cgcgccgacc cgaagatcat caacgcatcg cgccggctgc
     2581 ggatcgccaa cgggtcgggc gtgacggtgt cggaggtcaa ccagctcgtc gaccgattct
     2641 tcgaggctcg caagatgatg tcgcagatgg ccggccagat gggtttgccc gggttcggcc
     2701 gcaagagttc gcgaaaagcc aagggcggca aagggaaaaa gggcaagaag ggtgcgcggg
     2761 gaccgacgcc gccgaaggtg cgcggcccgc tgggcgccgg gatgcctggc ggggcgttcc
     2821 ccgacctgtc gcagatgccg cccgggctgg acgaattgcc gcctgcgctg gcggatttcg
     2881 atttgtcgaa gctgaagttc ccgaagaact agccgtgccg ctgcacgtgc ggggccgcgg
     2941 gttgccgagt ggggagcccg tcgagtggtg gatcatcgat gagcgctcgc gcgaggagca
     3001 tgggtacagc ggcacgctgt cggccgagcc ggtgtccggc gccgacaccg tgttcgacgg
     3061 cggctggatt ctgcccggtc tcgtcgacgc gcactgccac gtcggtgtcg gcgccgacgg
     3121 ccccgccgcc gacctcgacg aggcgatcgc ccaggcggag accgaacgca acgccggtgc
     3181 gctgctgctg cgcgactgcg gctcgccgat cgacacccgc agcctcgatg accacgacga
     3241 cctgccacgc atcatccgcg ccggacgcca cctggcccgg ccgaagcgtt acacgcccgg
     3301 actgcctatc gatgtcgagg atgaatcaca actaccggaa gccgttgcgg cacaagcccg
     3361 tcggggcgat ggctgggtga agctggtcgg cgactggatc gaccgcgagg tcggggacct
     3421 ggcgccgctg tggtccgacc acgtgctgaa agccgcgatc gacaccgccc acgcccacgg
     3481 cgcccgggtg acggcccacg tattcggcga agacgcgtta ccggggctcg tcacggccgg
     3541 catcgactgc atcgaacacg gcaccgggat caccgacgac gtcatcgatc tgatggtcga
     3601 acgcggcacc gcgttggtgc cgacgctgat caacatcgac aacttccccg gcatcgccga
     3661 ccgcgcggga aactacccgg tctacgccgc gcacatgcgc gacctgcaca cgacgagccg
     3721 gcatagggtc gccgccgcac gcgaggccgg ggtgccggtc tacgccggga ccgacgcggg
     3781 gtcgatgatc gtccacggcc gcatcgccga cgaagtcgac gccctgaagg gcgtcgggat
     3841 gagcccgacg gacgcgttgg gcgcggcgtg ctgggacgcg cggcggtggc tgggccggcc
     3901 ggggctcgag cacggcgcct cagcggatct gatctgcttc gccgacgacc cgcgcaccgg
     3961 ggcggcggtg ctgaaccggc cggacctggt gatcctgcgc ggccgcgtct ttgcgggcta
     4021 cagcgacccc acgctggcct gagggctttg cgcgaaaccc cagtcgagca ggctgatcgc
     4081 ttggtcccaa tatgtcggac cgccgggctt ctccaggccg tacatctgca cgacgataag
     4141 gcgccggccg ttgcgctcag ccgcgccgac gaaggtcttg cgagcgatgt tggtgaatcc
     4201 ggtcttgccg ccgagggcgc cgggataacg gcgcagcagc tcatcctggt tgacgatcgg
     4261 cgtctcaccc gcgtcggtcg ggaacagcgc ggagggctgg ctggtgatca tcgcgaacac
     4321 cgggttggcc aacgcggcgc ggaagatcac cgctaggtcg tgcggtgtgg tgcggaagtc
     4381 catgcccggc ccgtccagcc ccgacggtgt cgcagcgtgc gtgtcgtagg ccccgatctg
     4441 ttcggccttg gcgttcatct tcgccaccgc aacgtcgaac ccgccgagca tgtcagccag
     4501 cgtgtttgcc gcgtcgttgc ccgacaccag caggatcgcg tcgagcagct ggcgagcggt
     4561 gtaggtgcgg cccggcttga tgccgacgca gttgcactcc acctgggtgt cggcgacggt
     4621 cgcggtgacg gtgttgccca ggcccacctc gtcgagcgcg gtgagcgcca gcagcacctt
     4681 gttcgtgctg gcaggcgcat gcggtacgta cgcgtcgcgg gcggccagga tctggccggt
     4741 gtccatgtcg gccaccagcc atgccggtgc gggaccctcc ggcgcggcca cggcgccggc
     4801 cggctgaacc gccggatcag cgaacgcaac gggagcggac agtccgcatg ctgcgacaat
     4861 ggcagccagg ggggcgacga gctttcgcat ggctggtcag cctagccggg gggcgtcgat
     4921 ggtgttcaat caacgcatgt tgagcctgga ggagatctcg gaccggttgg aaatccagca
     4981 gctgctgatc gactactcga cggctatcga tcagcgccgc ttcgacgacc tggaccgcgt
     5041 gttcaccccc gacgcctaca tcgactaccg cgtgatgggc ggcatcgacg gcacgtatcc
     5101 cgcggtgaag gcctggctgg cccaggtgct gccgaacttt ccggtgtatg cccacatgct
     5161 cggcaacttc gacgtgcgga taagtggcga taccgcgtcg tcgcggacga tctgcttcaa
     5221 cccgatggtg ctcggcgggg acagcggcaa cgtgatgttc tgcgggctct ggtacgacga
     5281 cgagttcgtg cgcacacccg agggctggcg gatgacgcgc cgggtcgagg ccaagtgctt
     5341 cgacaaggtc ctgtagcgcc gactctgcgt tttcgtacgc cccatcggcg tgtcccgtac
     5401 gtatccacag tgtcgcggcc ggtcggcgcg atttcgccca ctgaccagcg ttctggcaca
     5461 atggtcggct ggtcagaccc acgcgaggca aaaccggatc cgcgcaaccg cacggcatcg
     5521 ctgaattgcc gcgtgacacc tacaggagtt tcaccatggc tgtgaagatc aagctcaccc
     5581 ggcttggcaa aatccgcaat ccccagtacc gcatcgccgt cgctgacgcc cgcacccgac
     5641 gcgacgggcg ggccatcgag gtcatcggcc gctaccaccc caaggaagag ccgagcctga
     5701 tcgagatcga ctccgaccga gcgcagtatt ggctgtccgt gggcgctcag cccaccgagc
     5761 cggtgctcaa gctgctgaag atcaccggcg actggcagaa gttcaagggc ctgcccggaa
     5821 ccgagggcac cctgaaggtg gccccgccga agcccagcaa gctggagctg ttcaacgccg
     5881 cgctggccga ggccgagggc ggcccgaccg ccgaagccac cacgccgaag aagaaggcgg
     5941 ccggagccgc gaaggcggcg acatcagaca gcgcgaatgc ccccgagaag gcggagaccg
     6001 ccgacatcga cacggcagag gcgaccgccg acgcgccggc cgaggccccg ccggccgcgg
     6061 acgccgaaag ctgagccggc gatgagctct gtcgtggttg atgcggtcga gcatctggtt
     6121 cgcggcatcg tcgacaatcc cgacgacgtt cgcgtcgaca tggtgaccaa ccgccgtggg
     6181 cgcaccgtcg aggtgcacgt ccaccccgac gacctcggca aggtcatcgg gcgcggcggg
     6241 cgtacggcga ccgcgctgcg cacgctcgtc gccggcatcg gcggtcgtgg catccgtgtc
     6301 gacgtggtgg acacagacca gtagccagca atggagctgg tcgtcgggcg ggtggtcaag
     6361 gcgcacggcg tgaccggtga gctggtcgtc gaggtgcgca ccgacgaccc ggacagccgc
     6421 ttcgcggtgg gatcgaccct gcgcggcaag ccccgacacg gcgcagcgcg ggagtacacc
     6481 gtcgagtcgg tccgcgacca cggcggccgg ctgctggtgc gcctggccgg aatcgacgac
     6541 cgcgacgctg ccgacgcgat gcgcggtgtc gtgttcgtga tcgactcggc tgagcttccg
     6601 cccaccgacg accccgacga gttctacgac caccagctcg agggccttgc ggtgcgcacc
     6661 gtcgacgggc gcgacgtggg cacggtcacc gaggtgctgc acaccgccgc gggggagctg
     6721 ctcgccattc gccgcgacga cgacggcgag tcgctggtgc cgttcgtgac cgcgatcgtc
     6781 acgtcggtct cgttggcgga ccgactgatt gagatcgacc cgcccgaggg tctgctggag
     6841 ctggcgtagc cgtgcgcatt gacgtcatca cgatctttcc gcagtacctg gagccgctgc
     6901 gggagtcgtt gccgggcaag gcaatcgacg ccgggatcgt cgacctgcgc gtgcacgatc
     6961 tgcggcagtg gacgcacgac gcgcaccatt cagtggacga cgcgccgtac ggcggcggcc
     7021 cggggatggt gatgacggcg ccggcatggg gcgaagctct ggacgaaatc tgttcggcgg
     7081 aaacacttct cgttgtgccc acgcccgcgg gtgtgccatt caaacagcag gacgctcagc
     7141 gctggagctc cgaagcgcgc ctcgtcttcg cgtgcggtcg ttacgagggg atcgaccagc
     7201 gggtgatcga cgacgccgcc cggcgcatgc gcgtggagga ggtctcgatc ggtgactacg
     7261 tgctgcccgg cggcgagtcg gccgcgctgg tgatgatcga ggcggtggtc cggctgctgc
     7321 cgcaggttct cggcaatccg gagtcacacc aacaggattc gtactccgac ggcctgctcg
     7381 aagggccggc ctacacccgc ccgccgagtt ggcgcgggct cgacgtgccg gatgtgctgc
     7441 tgtcaggcga ccacgcgcgc atcgcggcgt ggcgtcgtga acaggggctg cagcgcacgc
     7501 gtgagcgccg ccctgaccta ttggactaga tccgcccgcc cgggaacatc gtcttgacgg
     7561 cctgcgtgat cgtctcgcgc gccatgctgt cgtcggtggg ttgcatcgag ccaatggcca
     7621 tcacatagcg acggtcccaa ccaatcacgc cggtggactc gtgcatccag ttgccgccgt
     7681 tccagcagca catccagccc tgcttgaccg cgacgggctc ggcgtacaac gcgtcgggga
     7741 tgccgaactg ctgcgaatag ccgtcgatgc ccgtcggcgt cgactgcgcg aggttggaca
     7801 cgatgatgct ggcctgttgg gctggcagcc cgccgctgcc atcgagcagc ttgtcgtagt
     7861 agcggacgag gtcactcgcc gtgctcaagg tgttgaacca gcgcccgttg tagggggtcg
     7921 tcgtcgccgt caacccgtaa cgctcggtga cacgcgtgat gatctcactg ccaccactgc
     7981 ggttccagaa gttctcggcg gcgttgtcgt cggaggatcg cagcatgact tcgagcgccg
     8041 cgcgatcggc cggggagagc tgggtctgcc ccttggcttc ctgcagaagc aggtcgtcgg
     8101 cgatgaacag cttggccacc gaggcgatcg gaaaaggcga atcgtcgccg ttggacaccg
     8161 ttttgccagt cacccggtcc agcaccagca ccgagatctc cgcgccgctt gcagcggcct
     8221 gcagggtcgc ttgctgaatc cgggactcca gcccgtcgag agcgaccttg gcggtgtcgg
     8281 gcgggggggt gtccggcaac ggccgctggg cacccatggc gatgacgtcg cgggtcgctg
     8341 gtcggatccc cgttgccccg tgatctttgg cgacacacgc cccgacgagt acggctgacg
     8401 tcaggactgc gatcgctgtc cgaagtactg gccgcggccg ccgcatgtct ctcctcaacc
     8461 tattgggctt ctccaaaacg gcccgaccgc accgccgtcc gctatctcgg tagcctaatc
     8521 ggtttttcgg cgccttgggt cgtaagcgcg cgacgtcgtc tggtgtgttg atcttcacgc
     8581 tgtcggccgc gatttccctg cctgcggctg atctggcaca attgaccagt tgtctgcgtg
     8641 ggctgggagc cttcccgcga tgcagagatc aagacgtcag aacccgacgg cttcggctgc
     8701 gttgctcacg acgcgatgca ccccacgccg cgaacgcgag gaagaatttc gctatgaaca
     8761 cgctggactt cgtcgaccag acttcgctgc gcgacgacat cccggctttc aatcccggcg
     8821 acacggtcaa cgtgcacgtc aaggtcatcg agggttccaa ggaacgcatt caggtgttca
     8881 agggcgtcgt gatccgccgg cagggcggcg gaatccggga gacgttcacg gtccgcaagg
     8941 agagctacgg cgtgggtgtc gagcgcacgt tcccagtgca ctcaccgaac atcgaccaca
     9001 tcgaggtcgt cacacgcggc gacgtgcgcc gcgccaagct gtactacctg cgcgagttgc
     9061 gcggcaagaa ggccaagatc aaggagcggc gctgaccgcg gtagcttgat cccgtgacgg
     9121 acaagaccga atcggcagac gagaccgacg ctgaggccga ttccaaggcc agcaccgaag
     9181 acggcgacac gcccaagcgc aagaaatcgg tgcggtcggt actgatagag gccgcgatcg
     9241 tcatcgtggt cctggtggtc cccttctact tggtgcagac gttcgtcgcg cgcccgtatc
     9301 tgatcccgtc agagtccatg gagccgacgt tgcacgggtg tctcggatgc accggtgatc
     9361 gcgtggtggt cgacaaggtc atataccgct tcggctcgcc gcagcccggc gacgtgatcg
     9421 tcttcaaagg cccgcccgca tggaacgtcg gctacaagtc gatccgctcc gaaaatgccc
     9481 cggtgcggtg gctgcagaac accgtctcgt tcatcggatt cgtgccgccc gatgagaacg
     9541 acctggtcaa gcgcgtcatc gctgtcgggg gtcagaccgt ggagtgccga aacgccagcg
     9601 gactgacggt cgacggcaaa ccgctgaacc agccgttcct caacacgaag accatggggg
     9661 tcgagaacct cgacccggcc gtctaccggt gcctgggtcc ggagttcggt cccgtgaccg
     9721 tgccggacgg ccggct
//
LOCUS       DASWBF010000358        15368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_40126, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000358 DASWBF010000000
VERSION     DASWBF010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15368
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40126"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..601
                     /locus_tag="VF874_13235"
     CDS             119..601
                     /locus_tag="VF874_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9176989.1"
                     /translation="MAARPDGPFFVTGGGTQSLQRWDMTTGVLIGNPMPGHRDIVDDV
                     AVSSDGKLIVSGSKDATLRFWDADSGQPVGDPLTADSQSVATLVLSADRRILSLNINL
                     SPDETVSAWVWPAPAAWRHDLCNKLSYNMSDRQWSDWVSPDIGYKQLCPRLPKLADDN
                     "
     gene            complement(872..1903)
                     /locus_tag="VF874_13240"
     CDS             complement(872..1903)
                     /locus_tag="VF874_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9176990.1"
                     /translation="MTWAVLGAALLLVLMVAVAVWAYQTATRLDRLHVRYDLSWQALD
                     AALARRAVVSRAVGADAYGSGERCWRLTALADAAEQAPRAARETAENELSAELAMVDT
                     AVLGPALIAELADAEARVLLARRFHNDAVRDTLALRLRRPVRWLRLGGRAPLPTYFEI
                     AERGHVSDGDEGVVTLRTSARVVLLDEQGCVLLFCGSDPAREDVAAPKWWFTVGGQAR
                     PGETLQQAAAREVFEETGLRAAAADMVGPVWRRDAMMDFNNAVVRSHELFFVHRTRRF
                     DPSTTGHTALEHRYIHGYRWCDADDIAALVRAGEAVYPIQLGDLLAEADALASQPAGP
                     ERREPQPIR"
     gene            complement(1903..3027)
                     /locus_tag="VF874_13245"
     CDS             complement(1903..3027)
                     /locus_tag="VF874_13245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9176991.1"
                     /translation="MRIGMVCPYSFDVPGGVQSHVLQLAEVMRARGHEVSVLAPSSSQ
                     VPLPDYVVSGGRAVPIPYNGSVARLRFGPATHRKVKRWLADGQFDVLHLHEPNAPSLS
                     MLALAVAEGPIVATFHTSTTKSLTLSVFQGILRPFHEKIVGRIAVSDLARRWQMEALG
                     SDAVEIPNGVDVSSFANAPVLEGYPRPGRTVLFLGRFDERRKGMDVLLRALPALVEKF
                     PSVQVLIVGRGDEDELCEEAGPLAGHLRFLGQVDDDEKASAMRSADVYCAPNTGGESF
                     GIVLVEAMAAGTPVVASDLDAFRRVLRDGEAGRLVAVDDGDALAAGLIDVLGDDALRA
                     RYVEAAKTAVRRYDWSVVAQQIVRVYETVAAAGAKVEVAS"
     gene            complement(3028..3954)
                     /locus_tag="VF874_13250"
     CDS             complement(3028..3954)
                     /locus_tag="VF874_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEX9176992.1"
                     /translation="MRDRRAEVSPRLADWGYAAGWRVVRAMPDRLARRMFDAGALYAA
                     AGGGPEQLRKNLARVLGVPPSKVPDWLMRASVASYARYWREAFRLPSMDLKQVGKRLD
                     EHFYGAEQFDEALEAGRGAVAALPHSGNWDMAGVWLAQHHGTFTTVAERLKPESLARR
                     FIEFRETLGFEVVSHSGGDRAPFDVCAERLRQNKFVCLMADRDLTRTGVQVDFFGEPT
                     LMPAGPAKLAIATGARLLPAYLWNEADTCGIRVHPTLDTSSGDVHAITQALADQFTAD
                     IATHPADWHMLQPQWLADLSDERRARLAGDNL"
     gene            complement(3981..4634)
                     /locus_tag="VF874_13255"
     CDS             complement(3981..4634)
                     /locus_tag="VF874_13255"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9176993.1"
                     /translation="MSNFYLMTRAAYVKLSTPVAKVALKAGLTPDSVTIIGTAGTVIA
                     ALTLYPIGQLWWGSVAVSFFVLADMLDGAMARVRGGGTRFGSVLDAACDRIADGAVFC
                     GLLWWVAFGMNSPSLVVATLICLVTSQVISYIKARAEASGLRGDGGLIERPERLVMVL
                     VGAGLSDFPLFPLPWLLPIAMWLLAATSLITVAQRLHSVRTSPGATDMIENREKSES"
     gene            complement(4631..5194)
                     /locus_tag="VF874_13260"
     CDS             complement(4631..5194)
                     /locus_tag="VF874_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9176994.1"
                     /translation="MVDRGVGVPDRLQRLWTPYRMTYIAEAPFQREDTSPEQPFSEIP
                     TLPDEEGLVVARGERVYVVLNLYPYNPGHLMVVPYRRVSELEDLDEAESAELMAFTQK
                     AIRVIKAVSRPMGFNVGLNLGKVSGGSLADHLHMHVVPRWVGDANFITIIGQTKMIPQ
                     LLQETRQLLATEWAKQPGERSDGGIEH"
     gene            complement(5257..7311)
                     /gene="thrS"
                     /locus_tag="VF874_13265"
     CDS             complement(5257..7311)
                     /gene="thrS"
                     /locus_tag="VF874_13265"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9176995.1"
                     /translation="MTAAAPASPAAPVRVPAGTTAGAAVRDAGLPGRGEPAAVVVVRD
                     ADGRLRDLSWTPDTDVEAVPVAADTEDGRSVIRHSAAHVLAQAVQEMFPDAKLGIGPP
                     ITDGFYYDFDVAEAFTPEDLEALEKRMRQIVKEGQLFSRRVYESKDQALSELASEPYK
                     LELVDDKSGDPEVMEVGGDELTAYDNLNPRTRERVWGDLCRGPHIPTTRYIPAFKLTR
                     SSAAYWRGDQDNASLQRIYGTAWESQEALDRHLHLIEEAQRRDHRKLGVELDLFSFPD
                     ELGSGLPVFHPKGGIVRKEMEDYSRRKHVEAGYQFVNSPHITKEQLYQISGHLEWYAD
                     GMFPPMHIDAEFNEDGTLRKPGQNYYLKPMNCPMHHLIYRSRGRSYRELPLRFFEFGS
                     VYRLEKSGVVHGLTRVRGMTQDDAHIYCTREQLSSELTSLLQFVLELLADYGLDDYYL
                     ELSTKDPEKYVGSDEVWEEATNVLAEVGAASGLELVPDPGGAAFYGPKISVQVKDALG
                     RNWQMSTIQLDFNMPDRFELEYTAADGTRQRPVLIHRALFGSVERFFGVLTEHYAGAF
                     PAWLSPVQVVGIPIADEHVPYLEAVAAELNSRGARAEVDTSDDRMAKKIVHHTNQKVP
                     FLLLAGDRDVEAGAVSFRFRDRSQLNGVPRADAINMIVDWVQARENASPTADLLKPGG
                     AP"
     gene            complement(7372..7782)
                     /locus_tag="VF874_13270"
     CDS             complement(7372..7782)
                     /locus_tag="VF874_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02611 family protein"
                     /protein_id="HEX9176996.1"
                     /translation="MKAERRWARWRDKVRDRRAADFLYRIAVAVVGLAVLAVGILAIP
                     YPGPGWAIVFVGLAILATEFRWAQCLLAFAKQRYDKVMAWFRAQGLWVQAAGVVFTTA
                     VVVATLWLVGAIGWAAALFGLESAALRSPIGVGT"
     gene            complement(7779..8402)
                     /locus_tag="VF874_13275"
     CDS             complement(7779..8402)
                     /locus_tag="VF874_13275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9176997.1"
                     /translation="MATGVGEHPGGGFNPPVPTDKGGPDYGRLVEAVRTLQDHAKAVD
                     APDDVITRAAGLIEDVTALLAPYWADEWTSPSGRRLDLPNRGNILQVPAELAKTDDGR
                     VRGTAQFRRFHLGRNGAVHGGSIAMLFDSVLGYTAAKLTRSPYQRTAYLHVNYRQIVP
                     VEKELQVDAGIERIDGRKIFVEGRITDGDAVLADGEALFVRLRPGQP"
     gene            8532..8966
                     /locus_tag="VF874_13280"
     CDS             8532..8966
                     /locus_tag="VF874_13280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9176998.1"
                     /translation="MSEPLTDEQQRKLRLSLRDGMPKTPFMGWLGIVFDRYEPDDVVI
                     RLPFREDLTNDGIYFHGGVIASVLDTAGAAAAWSNHDFCKGTRASTVGMTVNYVGAAK
                     RSDLLCHGRTVRRRKELIFTEITATDADGEVVAHAVQTYRIV"
     gene            complement(8951..9529)
                     /locus_tag="VF874_13285"
     CDS             complement(8951..9529)
                     /locus_tag="VF874_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9176999.1"
                     /translation="MTSPRVPRLAVDEAKAAADEAGVPNYMAELAIFQVLLNHPTLAR
                     RINDLLATMLWHGSLDIRLRELVIMRIGWLTASDYEWTQHWRVALGLGVSADDLLGVR
                     DWKAHNGFGPGERAVLAATDDVVCDGAVSAESWAACERELGPDPAVLVELVTAIGAWR
                     MVATILHSLAVPLEDGVDSWPPDGRAPSQTIR"
     gene            complement(9526..10623)
                     /locus_tag="VF874_13290"
     CDS             complement(9526..10623)
                     /locus_tag="VF874_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9177000.1"
                     /translation="MDRSARALVLEAPRRLDLRRLPIPDVGDDDGLVRVEACGLCGTD
                     HEQYTGELAGGFAFVPGHETVGVIEAIGSRAAARWGVAAGDRVAIEVFKSCRECAACR
                     SGEYRRCERHGIADMYGFIPVDRAPGLWGGYAEYQYLAPDSMVLEVPHGLNPVVATLF
                     NPLGAGIRWGATLPGTSPGDVVAVQGPGVRGLCAAAAAKEAGAGFVMVTGLGARDANR
                     LTLAREFGADLVVDVARDDPVGALRNATGGLADVVVDVTAKAPAAFAQAIALARTAGT
                     VIVAGTRGWGSDAPGFSPDVLVYKELNIVGALGVDAPAYRAALELLASQRYPFESLPR
                     RCVGLDGAADLLATMGGERDEVPPVHGVLTP"
     gene            complement(10623..11489)
                     /locus_tag="VF874_13295"
     CDS             complement(10623..11489)
                     /locus_tag="VF874_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9177001.1"
                     /translation="MTTQTVAQASGTFTFGGDLTVNRLGFGAMRLTGKGVWGPPADHD
                     ECIRVLRRAVELGVNFVDTADSYGPHVSEELIREALHPYPDDLVVATKAGLVRPGPND
                     WRPLGFPAYLRQQCELSLRRLGVEAIDLFQLHRIDSKFPAEDQIGELAALQQQGKIRH
                     IGLSEVSVDQLHAARKVATIVSVQNMYNLSVRGAEPVLEACEADGIGFIPWFPLAAGP
                     LAATDGPLQRIAAERRATPSQLALAWLLKRSPVILPIPGTSKVAHLEENVAAAEITLS
                     DEEFDALSNAGG"
     gene            11542..12462
                     /locus_tag="VF874_13300"
     CDS             11542..12462
                     /locus_tag="VF874_13300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014876826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9177002.1"
                     /translation="MKFGFPLPHTMRLKALTQPWEYSVTGADQAVMARRAEELGYEMI
                     AVPEHFVVPNTHLELSGPHYFHSTVAQAFLAGATKRIQVNSCVTLLPLQHPIVLAKAL
                     STADWMSGGRIGVTFGVGWDEEEFNILGVPFHERGRMADEYLAAIIELWTSDSPSFDG
                     KYVSFENVAFEPKPLQQPHLPIWIGGDAPAALRRAARFASGWFPFLTQPDEIPAKIDY
                     IKSQPTFTGEPLEVVYGLGTSLVGERHVVVDDPTQQPGMTAPEIIDRLGRFAALGVTM
                     TSVPIPPVRGVDEYLDYAQWVIEEIKPKVP"
     gene            12468..12965
                     /locus_tag="VF874_13305"
     CDS             12468..12965
                     /locus_tag="VF874_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HEX9177003.1"
                     /translation="MNLARLSDEPFTYTEIGATAGRLPDGYHHVSKSARIGTGRARFE
                     EAAARVMRWGMLRGAGVRPRPSTEVAQAGSVVVVGLGPVRAPCRVVYVVDEPDRRGFA
                     YGTLPGHPESGEELFAVRHDPADDAVYAEVVAFSRHATWWSRAAGPVTSLIQRIVTAR
                     YVEAV"
     gene            13009..13715
                     /locus_tag="VF874_13310"
                     /pseudo
     CDS             13009..13715
                     /locus_tag="VF874_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246620.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
     gene            13776..14681
                     /locus_tag="VF874_13315"
     CDS             13776..14681
                     /locus_tag="VF874_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9177004.1"
                     /translation="MAQGLVILFALLSAVCLAIGIVVRQRATIDVPQEAGVGATMITA
                     LLRKRLWWIGSGVAALGYVCQAVALSHGSLLVVQPLLVSSLLFALPLSARLAGRRVTP
                     AEWAWAVMLTAGLALFVLMARPRPGLYRGQELAWTVAIAVIAPIVLFCVVFGARSTGR
                     RRAVLIAIAVGVMFGLSAVLTKLVMFELAEDGAVAVLKLPSLYLLAAVGLTATFLQQS
                     AFHSGALQTSVPTMLVLEPVVAVLVGAFVLNEHLSVSGYEMVALPLAVGAMAAATIAL
                     GRDEGAYEEQLEAALAGRAESSSRP"
     gene            14902..>15368
                     /locus_tag="VF874_13320"
     CDS             14902..>15368
                     /locus_tag="VF874_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9177005.1"
                     /translation="MSHPKVDRQAGVPEGSTSYYFRTRTALLRAVAERLAELDLAELK
                     SVTEATRVDNGRRSAPRASRLATVVMRSATEPGLTRTKARFELLLQANRDPVLADLFR
                     HNTEIFTELHREIVERWQPPGADVDSSVIDDQTYVTMNFISGVMMSLAAGDRT"
BASE COUNT         2473 a         5299 c         5157 g         2439 t
ORIGIN      
        1 ttcagccccg acggacaacg gctcgtgtcc ctgagcgcgt actcacttcg accgtgggac
       61 accaggaact ggcaactcgt cggcaagccg catctcagct ccaacattct tcggcagttt
      121 ggccgcccgc ccggatggcc ctttctttgt caccggcggc ggcacgcaaa gccttcagcg
      181 atgggacatg accacgggtg tgctgattgg gaatcccatg cccggtcacc gtgacatcgt
      241 cgacgatgtc gccgtcagca gcgacggaaa gttgatcgtc tccgggagta aggatgcgac
      301 actccggttc tgggatgcgg actccggtca gcccgttggg gatccgttga ccgccgactc
      361 tcaaagcgtc gcgactcttg tcctgagtgc ggaccggcgc atcctctcat tgaacatcaa
      421 cctctccccc gacgaaaccg tgtcggcatg ggtctggccg gctcccgcgg cctggcggca
      481 cgatctctgt aacaagttga gctacaacat gagtgaccgg caatggtctg actgggtgtc
      541 accagacatc ggttacaagc aattgtgccc gcgtctgccc aagcttgccg acgacaactg
      601 actctcagca cggtttggcc tgcgggtcgt gcggcgcaac gatgtgcagc cggcgggcgc
      661 gttcgcgtcg gcgctgacgg gaagtggcag cgccccgctt gccgactaat caccatgcgc
      721 cggatctcgt tctgctgcac gcagtttcat gccgaagata tcgcgtcgac gatttcatag
      781 tggccactga gaccgtgagt agttcggaat atcgaccctc gggcggcggc tatctacgtg
      841 ccgagaggtc gcagatgcca ggttaactat gtcagcggat cggctgcggt tcgcggcgtt
      901 cgggacccgc gggctgggag gccagcgcgt cggcttcggc gagcaggtca ccgagctgga
      961 tcgggtacac cgcctcaccg gccctcacca gcgcggcgat gtcgtcggcg tcacaccagc
     1021 ggtatccatg gatgtagcga tgctcgagcg cggtgtggcc cgtggtcgac gggtcgaacc
     1081 gccgggtgcg gtgcacgaag aacagctcgt ggctgcgcac caccgcgttg ttgaagtcca
     1141 tcatcgcgtc gcgtcgccac accggcccga ccatgtccgc ggcggcggcg cgcagtcccg
     1201 tctcctcgaa cacctccctg gccgcggcct gctgcagcgt ctcccccggc cgcgcctggc
     1261 cacctacggt gaaccaccac ttgggtgcgg caacgtcctc acgcgcgggg tcggatccgc
     1321 agaacagcag cacacacccc tgctcgtcga gaagcacgac gcgcgccgac gtgcgcaggg
     1381 tgacgacgcc ctcgtcgccg tccgacacgt gcccgcgctc tgcgatctcg aaatacgttg
     1441 gcagcggcgc tcttccgccg aggcgtagcc atcggacggg ccgtcgcaac cgcagcgcca
     1501 gcgtgtcgcg taccgcgtcg ttgtggaacc gccgggccag cagcacccgc gcctcggcat
     1561 cggccagctc ggcgatcagc gccggcccca ggacagcggt gtcgaccatc gccagctccg
     1621 cggacagctc gttttcggcg gtttcccgcg ccgcccgcgg cgcctgctcg gcggcgtcgg
     1681 ccaacgccgt cagccgccag caccgctcac cgctgccgta cgcgtcggcg cccacagcgc
     1741 gcgagaccac cgcccgccgg gccaacgcgg cgtccagtgc ctgccaggac aagtcgtagc
     1801 ggacgtgcag ccggtccagc cgcgtcgcgg tctggtacgc ccacacggcg acggcaacca
     1861 taagaaccaa cagcagcgcc gcgcccagca ctgcccaggt catcagctgg ccacctcgac
     1921 cttggcgccc gccgcggcga cggtctcgta cacccgcacg atctgctggg cgaccaccga
     1981 ccagtcgtag cggcgcaccg cggtcttggc cgcctcgacg tagcgcgccc gcagcgcgtc
     2041 gtcgccgagc acgtcgatca gccctgcggc cagcgcgtcg ccgtcgtcca cggccaccag
     2101 ccggccggcc tcaccgtcgc gcagcacgcg gcggaacgcg tcgaggtcgc tggccaccac
     2161 cggcgtgccc gcggccatcg cctccacgag cacgatcccg aagctctccc cgccggtgtt
     2221 gggcgcgcag tacacgtcgg cgctgcgcat ggccgacgcc ttctcgtcgt cgtccacctg
     2281 tcccaggaac cggaggtgac ccgcgagcgg cccggcttcc tcgcacagct cgtcctcgtc
     2341 accgcggccg acgatcaaca cctgcacgct agggaacttt tccaccagcg cgggcagcgc
     2401 acgcagcagc acgtccatgc ccttgcgccg ttcgtcgaag cggccgagga acagcacggt
     2461 cctgccgggc cgcggatagc cttccagcac aggcgcattc gcgaacgacg acacgtccac
     2521 gccgttgggg atctccaccg cgtcggagcc cagcgcctcc atctgccagc ggcgggccag
     2581 atccgacacg gcgatgcggc cgacgatctt ctcgtggaac ggccgcagga tgccctgaaa
     2641 caccgacagc gtcagcgatt tggtggtgga cgtgtgaaac gtcgcgacga tcgggccttc
     2701 cgcgaccgcc agggccagca tcgacaggct cggcgcgttg ggctcgtgaa ggtgcagcac
     2761 gtcgaactgg ccgtcggcca gccagcgctt gaccttgcgg tgcgtggccg gaccgaaccg
     2821 cagccgggcg acggagccgt tgtacgggat cggcaccgcc cggccgccgg agacgacata
     2881 gtcgggcagg gggacctgcg acgacgacgg cgcgagcacg ctcacctcgt ggccgcgggc
     2941 gcgcatcacc tcggcgagct gcagcacgtg cgactggacc ccgcccggga cgtcgaacga
     3001 gtacgggcac accatcccga tgcgcattta cagattgtct cccgccaacc tcgcgcgccg
     3061 ctcgtcggac aggtcggcca gccactgcgg ctgcagcatg tgccagtcgg ccgggtgcgt
     3121 tgcgatgtcg gcggtgaact gatcggccag cgcctgggtg atcgcgtgga catcaccgga
     3181 gctggtgtcc agggtcggat gcacccggat gccgcaggtg tctgcttcgt tccagaggta
     3241 cgccggcagc agcctggcgc cggtggcgat cgccagcttg gccggtcctg ccggcatgag
     3301 ggtgggctcg ccgaagaagt cgacctgcac gccggttcgg gtcaggtcgc ggtcggccat
     3361 caggcacacg aacttgttct ggcgcagccg ttccgcacac acgtcgaacg gtgcacggtc
     3421 gcctccggaa tgcgacacca cttcgaagcc cagggtctcg cgaaactcga tgaatcgacg
     3481 agccagcgac tcgggcttga gccgctccgc gacagtggtg aaggtgccgt ggtgttgcgc
     3541 caaccaaact cctgccatgt cccagttgcc gctgtgcgga agcgcggcga cggccccgcg
     3601 accggcttcc agtgcctcat cgaattgttc tgcaccgtaa aagtgttcgt cgagccgctt
     3661 gcccacttgc ttcagatcca tcgacggcaa ccggaacgcc tcgcgccagt agcgggcata
     3721 ggacgccacc gacgcccgca tcagccaatc gggcaccttc gacggcggca cgccgagcac
     3781 ccgggccaag ttcttgcgca gctgctcagg tccgccaccc gccgcggcat agagcgcacc
     3841 ggcgtcgaac atgcggcgcg ccaggcgatc tggcatggca cggaccacgc gccagccggc
     3901 ggcatagccc cagtcggcga gccgcgggct cacctcggca cggcggtcgc gcatgagccg
     3961 cagtctgggc gacgtcggaa tcacgactcg ctcttctccc ggttctcgat catgtccgtc
     4021 gcgccgggcg acgtgcggac ggagtgcagg cgctgcgcca ccgtgatcag gctggtcgcc
     4081 gcaagcagcc acatcgcgat cggcagcagc cacggcagcg ggaacaacgg gaagtcggac
     4141 agcccggcgc cgaccagcac catgaccagc cgctccgggc gttcgatcag cccgccgtcg
     4201 ccgcgcaggc cgctggcctc ggcgcgcgcc ttgatgtacg agatcacctg cgacgtgacc
     4261 aggcagatca gcgtggccac caccagcgac gggctgttca tgccgaacgc cacccaccac
     4321 agcaggccgc agaacaccgc cccgtcagcg atccggtcgc acgcagcgtc cagcaccgag
     4381 ccgaaccggg tgccgccgcc gcgcactcgc gccatcgccc cgtcgagcat gtcggccagc
     4441 acgaagaacg agacagccac cgagccccac cacagctgac cgatcgggta caacgtcagc
     4501 gcggcgatca ccgtgcccgc ggtgccgatg atcgtcacgc tgtcgggagt caggccggct
     4561 ttcagcgcga ccttggccac cggagtggac agcttgacgt acgccgcgcg cgtcatcagg
     4621 tagaagttgc tcagtgctca atccccccgt cgctccgctc ccccggttgt ttcgcccact
     4681 cggtcgccag cagctgccgc gtctcttgca gcagctgcgg gatcatcttc gtctggccga
     4741 tgatcgtgat gaagttggcg tccccgaccc atcgcggaac cacgtgcatg tgcaggtggt
     4801 cggccagcga tccgccggac accttgccca ggttcaggcc cacgttgaac cccatcggcc
     4861 gtgacacggc cttgatcacg cgaattgcct tctgcgtgaa cgccatcagc tccgcgctct
     4921 cggcctcatc gaggtcctcg agctccgaca cgcggcggta cggcaccacc atcaggtgcc
     4981 cggggttgta cgggtagagg ttgagcacca cgtacacccg ttcgccgcgg gccacaacga
     5041 ggccttcctc gtcgggaagc gtcgggatct cgctgaacgg ctgctcgggg gacgtgtcct
     5101 cgcgctggaa gggcgcctcg gcgatgtagg tcatccggta gggcgtccac agccgctgca
     5161 gccggtcggg cacgccgacg ccgcggtcga cgatggcgtc gtcgcagctt cccgctcttc
     5221 gcgcaagcgg ctcatcgcca gtgggttcgg gttgggtcac ggcgctccac ccggcttcag
     5281 cagatcggct gtcggcgaag cattttcccg cgcttgcacc cagtcgacga tcatgttgat
     5341 cgcgtcggcg cggggcacac cgttgagttg gctgcggtca cggaaccgga agctgaccgc
     5401 gccggcttcc acgtcgcggt caccggccag cagcagaaac ggcaccttct ggttggtgtg
     5461 gtggacgatc ttcttggcca tccggtcgtc gctggtgtcc acctcggccc ttgcgccccg
     5521 tgaattgagc tcggcggcaa cggcttccag gtagggcacg tgctcgtcgg cgatcgggat
     5581 gccgaccacc tgcaccggcg acagccatgc gggaaacgcg ccggcgtaat gctcggtgag
     5641 cactccgaag aaccgctcca ccgacccgaa cagcgcgcgg tgaatcagca ccggccgctg
     5701 gcgggtgccg tcggcggcgg tgtactccaa ctcgaaccgg tccggcatgt tgaagtccag
     5761 ctggatcgtc gacatctgcc agttgcggcc cagcgcgtct ttgacctgga cggagatctt
     5821 cggcccgtaa aacgctgcgc cacccgggtc gggcaccagc tcgaggcccg acgcggcgcc
     5881 gacctcggcc agcacgttgg tggcctcctc ccacacctcg tcagagccca cgtacttttc
     5941 cggatccttg gtggacagtt ccaagtagta gtcgtccagg ccgtagtcgg cgagcagctc
     6001 aagcacgaac tgcagcaatg acgtcagctc actgctgagc tgctcgcggg tgcaatagat
     6061 gtgcgcgtcg tcctgggtca tcccgcgcac tctggtgagg ccgtgaacga caccggactt
     6121 ctcgaggcgg tacacactgc cgaactcgaa gaaccgcagc ggcagttcgc ggtacgaccg
     6181 cccgcgcgag cggtaaatca gatggtgcat cgggcagttc atcggcttga ggtagtagtt
     6241 ctgcccgggc ttgcgcagcg tgccgtcctc gttgaactcg gcgtcgatgt gcatcggcgg
     6301 gaacatgccg tcggcgtacc actcgaggtg ccccgagatc tgatacagct gctccttggt
     6361 gatgtgcggg gagttgacga actggtagcc ggcctcgacg tgcttacgcc gggagtagtc
     6421 ctccatctcc ttgcggacga tgccgccctt gggatggaaa accggtaggc ccgaaccgag
     6481 ttcgtcgggg aagctgaaca ggtcgagctc cacgccgagc ttgcggtggt cgcggcgttg
     6541 cgcctcttcg atcaggtgca ggtgccggtc cagcgcctcc tgagattccc acgccgtgcc
     6601 gtagatgcgc tgcaggctgg cgttgtcctg atcgccgcgc cagtacgccg ccgagcttct
     6661 ggtgagcttg aacgcgggga tgtagcgggt ggtcgggatg tgcgggccgc ggcacaggtc
     6721 gccccaaacc cgttcgcggg tacggggatt caaattgtcg taggcggtga gttcgtcgcc
     6781 gccgacttcc atcacttccg ggtcgccgga cttgtcgtcg acgagttcga gcttgtaggg
     6841 ctctgaggcc aactcgctaa gggcctgatc cttggattcg tagacgcgcc gggagaacag
     6901 ctggccttct ttgacgatct ggcgcatccg cttctccagc gcttccagat cctcaggagt
     6961 gaacgcctcg gcgacgtcga agtcgtagta gaagccgtcg gtgatcggcg ggccgatccc
     7021 gagcttggcg tcggggaaca tctcctgcac ggcctgcgcc agcacgtggg cggccgagtg
     7081 ccggatgacg ctgcggccgt cctcggtgtc ggcggcgacg ggcacggcct cgacgtcggt
     7141 gtcgggcgtc cagctcaggt cgcgcaaccg gccgtcggcg tcgcggacga cgacgaccgc
     7201 ggcgggctca ccgcggccgg gcagtcccgc gtcgcggacc gctgccccgg cggtggtccc
     7261 ggcaggcacc cggacggggg ctgcggggct tgcgggtgcg gcggcggtca tcgcggaatc
     7321 tccaacaggg tcaatgtgct cgatcgcgca ccatgctatc ggtgacgccg gttacgtccc
     7381 cacgccgatc gggctgcgca acgctgccga ttccagcccg aacagcgccg cggcccagcc
     7441 gatggcgccg acgagccaca gcgtcgccac caccaccgcg gtggtgaaca cgacaccggc
     7501 ggcctgcacc cacaggccct gcgcacggaa ccacgccatc accttgtcgt agcgctgctt
     7561 cgcgaacgcc agcagacact gcgcccaacg gaactcggtg gccaggatgg cgaggccgac
     7621 gaacacgatc gcccagcccg gccccggata cgggatcgcg aggatgccga cggcgagcac
     7681 ggccaggccg acgaccgcga cggcgatccg gtagaggaag tcggccgccc ggcgatcgcg
     7741 caccttgtcg cgccaccgtg cccaccggcg ctcggctttc atggctggcc gggccgcagc
     7801 cgcacgaaca gcgcctcgcc gtcggccagt accgcgtcac catcggtgat ccggccttcg
     7861 acgaagatct tgcggccgtc gatgcgttcg atgccggcgt cgacctgcag ctccttttcg
     7921 acgggcacga tctggcggta gttgacatga agataggcgg tgcgctggta cgggctgcgg
     7981 gtgagcttgg cggcggtgta gccgagcacc gagtcgaaca gcatcgcgat cgatccgccg
     8041 tggacggcgc cgttgcggcc caggtggaat cggcggaact gcgcggtgcc gcgcacccgc
     8101 ccgtcgtcgg tcttcgccag ctcggccggc acctgaagga tgttgccgcg gttgggcagg
     8161 tccaaccgtc gacccgacgg ggatgtccac tcgtcggccc agtacggcgc cagcagcgcg
     8221 gtgacgtcct cgatcaagcc ggcggcgcgg gtgatgacgt catcgggggc gtcgacggcc
     8281 tttgcgtgat cctgcagggt ccggacggct tcgacgagcc ggccgtagtc gggtccgccc
     8341 ttgtccgtcg ggacaggcgg gttgaagccg ccgccggggt gctcgccgac tccagttgcc
     8401 acccgggtca cgctacgtga ggtgtgctct cgcgagcgtg cggccactgc gaaaatcgcg
     8461 cccccgaatt tcgcagtggg cgcacgctcg gcgtgcgcag actctgcgct gcgaccacgt
     8521 acagttcggc catgagcgag ccgttgaccg acgagcagca gcgcaagctc cggctgtcgc
     8581 tgcgggacgg gatgccgaag acgccgttca tgggatggct cggcatcgtg ttcgaccgct
     8641 acgaacccga cgacgtcgtc atccggctgc cgtttcgcga agacctgacc aacgacggca
     8701 tctacttcca cggcggcgtg atcgcgtcgg tgctcgacac cgcgggcgcc gcggcggcat
     8761 ggtccaatca cgacttctgc aagggcaccc gcgcgtcgac cgtcgggatg acggtcaact
     8821 acgtcggcgc cgccaagcgc tctgatctgc tgtgccacgg ccgcaccgtg cgtcgccgca
     8881 aggagctgat cttcaccgag atcaccgcca ccgacgccga cggcgaggtg gtggcccacg
     8941 ccgtgcagac ctaccggatc gtctgactcg gggctcgccc gtcgggcggc cagctgtcga
     9001 caccgtcctc cagcggcacc gcgaggctgt gcaggatcgt cgcgaccatc cgccacgccc
     9061 cgatcgcggt gaccaactcg accagcacgg cgggatccgg gccgagttcg cgttcgcacg
     9121 ccgcccagct ttcggcactg accgccccgt cgcacaccac gtcgtcggtg gccgccagca
     9181 cggcccgctc tccggggcca aaaccgttgt gcgccttcca gtctcgcacg ccgagcagat
     9241 cgtcggcgga cacgccgagc ccgagtgcga cgcgccagtg ctgggtccac tcgtaatccg
     9301 acgcggtgag ccacccgatg cgcatgatca ccagctcgcg cagccggatg tcgaggctgc
     9361 cgtgccacag catcgtcgcg agcaggtcgt tgatgcgccg ggccagcgtg gggtggttca
     9421 gcagcacctg gaagatcgcg agttcggcca tgtagttggg cacacccgcc tcgtcggcgg
     9481 cggccttcgc ttcgtcgaca gccagacgtg gcacccgcgg agaggtcacg gcgtcagcac
     9541 cccatgcaca ggcggcacct cgtcgcgctc gccgcccatc gtggccagca ggtcggccgc
     9601 gccgtctagc cccacgcagc gccgcggcag ggattcgaag ggataccgct gcgacgcaag
     9661 cagttccagc gccgcgcgat aggcgggcgc gtccacgccg agcgctccga cgatgttcag
     9721 ctccttgtag accaggacgt ccggcgagaa accgggagca tcgctgcccc agccacgggt
     9781 gccggcgacg atcacggttc ctgcggtgcg ggccaacgct atcgcttggg caaacgctgc
     9841 gggcgctttc gcggtgacgt cgaccaccac atctgccagt ccgccggtcg cgttgcgaag
     9901 ggcgccgacc ggatcgtcgc gtgccacgtc cacgacaagg tcggcgccga actcacgggc
     9961 aagcgtcaac cggttcgcgt cgcgcgcacc caatcccgtc accatcacga acccggcgcc
    10021 ggcctccttc gcggcggccg ccgcgcacag accgcggacg ccgggtccct gcacagcgac
    10081 cacgtcacct ggcgatgtgc cgggaagcgt tgcaccccag cggattccag cgcccagcgg
    10141 gttgaacagc gtcgccacca cggggttcag cccgtgcggc acctcaagca ccatcgagtc
    10201 gggcgccaag tactggtact cggcgtaccc gccccacaaa ccgggggcac ggtcgaccgg
    10261 gatgaacccg tacatgtcgg cgatgccgtg ccgctcgcag cggcggtact caccggagcg
    10321 gcacgccgcg cattcccggc acgacttgaa cacctcgatc gccacccggt cgccggccgc
    10381 cacgccccac cgcgcagcag cccgcgagcc gatcgcctcg atcacgccga cggtctcgtg
    10441 gccggggacg aacgcgaacc cgcccgccaa ctcgccggtg tactgctcgt ggtcggttcc
    10501 acacagcccg cacgcctcca cgcgcacgag cccgtcgtcg tcgccgacgt cggggatcgg
    10561 caacctccgc agatcgagtc ggcgcggcgc ttccagcacc agtgcccgcg cggaccggtc
    10621 catcagccgc cggcgttgct caacgcgtcg aattcctcgt cggacagcgt gatttcggcg
    10681 gccgcgacgt tctcctcaag atgggcgacc ttcgacgtgc ccggaatcgg caggatcacc
    10741 ggcgaccgct tcagcagcca ggccagcgcc agctgcgacg gggtggcgcg ccgttcggca
    10801 gcgatccgct gcagcggccc gtcggtggcc gccagcggtc cggccgccag cgggaaccac
    10861 ggaatgaacc cgatgccgtc ggcctcgcac gcttcgagca cgggttcggc gccgcgcacg
    10921 gacaggttgt acatgttctg caccgacacg atcgtcgcga ccttccgggc ggcatgcagc
    10981 tggtcgacgc tgacctcgga cagcccgatg tggcggatct tgccttgctg ctgcagtgcg
    11041 gccagctcgc cgatctggtc ctcggccggg aacttcgagt cgatgcggtg cagttggaac
    11101 aggtcgatgg cctcgacccc gaggcggcgc aggctcagct cgcactgctg gcgcaggtac
    11161 gccgggaacc cgagcggacg ccagtcgttg ggaccggggc gcaccaaacc ggccttggtg
    11221 gcgacgacga gatcgtcggg atacgggtgc aacgcctcgc ggatcagctc ctcggagacg
    11281 tgcgggccgt atgagtcggc ggtgtcgacg aagttcaccc cgagctcgac cgcgcgacgc
    11341 agcacgcgaa tgcattcgtc gtggtcggcg ggcgggcccc atacgccctt gccggtgagg
    11401 cgcatggcgc cgaacccgag gcggttgacg gtcagatctc cgccgaacgt gaacgttccg
    11461 gaggcttgcg cgacggtttg tgtggtcact ttctcgaccg tacgccgccg aactggatgc
    11521 atcagcgcga tgatggcagt catgaaattc gggttcccgc ttccgcatac gatgcggctg
    11581 aaggccctga cgcagccgtg ggaatacagc gtgactggcg cggatcaggc cgtcatggcc
    11641 cgacgcgccg aagagctcgg ctacgagatg attgccgtcc cggagcactt cgtggtcccg
    11701 aacacgcacc tggagctgtc ggggccgcac tacttccact cgaccgtcgc acaggccttc
    11761 ctcgccgggg cgacgaagcg cattcaggtg aactcgtgcg tcacgctgct gccgctgcag
    11821 catccgatcg tcctggcgaa ggcgttgtcc accgcggatt ggatgagcgg cggtcgcatc
    11881 ggcgtcacgt tcggcgtcgg ttgggacgag gaagagttca acatcctcgg cgtgccgttt
    11941 cacgaacggg gacgcatggc cgacgaatac ctggccgcga tcatcgagtt gtggaccagc
    12001 gattcgccga gtttcgacgg caagtacgtc tcgttcgaga atgtcgcgtt cgagccaaaa
    12061 cccctgcagc agccgcattt accaatctgg atcggcggcg acgccccggc ggcgctgcgg
    12121 cgcgcggcac gattcgcgtc ggggtggttc ccgttcctca cccagccgga cgagattccc
    12181 gccaagatcg actacatcaa gtcgcagccc accttcacgg gcgagccgtt ggaagtggtg
    12241 tacggcctgg gcacatcgct cgtcggtgaa agacatgtcg tcgtcgacga ccccacccag
    12301 caacccggca tgaccgcacc ggagatcatc gaccggctcg gccggttcgc ggcgttgggc
    12361 gtgacgatga ccagcgtccc gatcccaccg gtcagaggtg tcgacgaata cctcgactat
    12421 gcccagtggg tgatcgagga gatcaagcct aaagtcccgt aaatgaaatg aatctggccc
    12481 ggctgagcga cgaaccgttc acctacaccg agattggtgc gacggccggc cggctgcccg
    12541 acggatatca ccatgtgagc aagtcggcgc ggatcggcac cggccgggcg cggttcgagg
    12601 aggcggcggc gcgggtgatg aggtggggca tgctgcgcgg agccggggtg cgtccgaggc
    12661 ccagcaccga ggtcgcgcag gccggttcgg tggtggtcgt gggcctcgga ccggtaaggg
    12721 ccccatgccg cgtggtgtac gtcgtcgacg agcccgaccg ccgcggcttc gcgtacggca
    12781 cgctgcccgg ccacccggaa tccggcgaag agctgttcgc cgtgcgccac gaccccgccg
    12841 acgacgccgt ctacgccgag gtggtggcgt tctcgcggca cgcgacctgg tggagcaggg
    12901 ccgccgggcc ggtcacgtcg ctgatccagc ggatcgtgac ggcgcggtac gtagaagccg
    12961 tgtgaccaat gaccggcgac ccttcagggc gccgcgtacc gttgacccgt gagccagccg
    13021 ctggaagccc acagcgccta ccgccgcatc gccgagatcg tgcgcgcgct gcatgaccgg
    13081 ccggagacgg acgcagacac ggtcatcgcg gagctggccg agcatgccgc cctcgagatt
    13141 cccggggcca agtacgcggg tgtcacgatc accaccaacc gcaagcgggt cgacaccccg
    13201 gcggccacgc acccgtacgc ggtcacgctc gaccggattc agcagcggca ccgcgaaggg
    13261 ccgtgcctgg agtcggcgtg ggacaagaag acgattcaca tcgccgacct ggagtcggac
    13321 ttccggttcc cgaaataccg cagggacgcg ctggtggaga caccgatccg gtcgatcatg
    13381 gcgtttcagc tgttcatcgc cgaccagacg atgggcgcgc tcaacgtcta cgccgaggag
    13441 ccgcacgcct tcaacgacga gagcgaggaa gtgggactga tctcgcgacg cactcggcgg
    13501 tggcgtggaa ctcggcccgc cgcgacgagc agttccacca ggcgctggcc agccgggacg
    13561 ccatcggcca ggcgaagggc atgatcatgg agcgctacgg aatgggggcg atccaggcgt
    13621 tcgagatgtt gaggaagttg tcgcaagact ccaacgtgcc tctggcccga atcgcccagg
    13681 agctaatcga gaagtcacac ccgtcggcaa gctagcctga gttgccgatt aagagccctc
    13741 gggtcccagc ggccggagat gactgagagg cattaatggc gcagggactg gtgattctct
    13801 tcgcgttgct gtctgctgtc tgcttggcga tcggaatcgt ggttcgtcag cgcgccacca
    13861 ttgacgtccc gcaagaagcg ggcgtcggcg caacgatgat cacggcgttg ctgcgcaagc
    13921 ggctgtggtg gataggttcc ggggtggcgg cgttggggta tgtgtgccag gccgtggcgt
    13981 tgtcgcacgg gtcgttgctg gtggtgcagc cgctgctggt gtcgtcgctg ctgttcgctc
    14041 tgcctctgtc cgcacggctg gccgggcgcc gcgtcactcc ggcggaatgg gcgtgggcgg
    14101 tgatgttgac cgccgggctg gcgctgttcg tgctgatggc gcgcccgcgg ccgggtctgt
    14161 accgcggcca ggagctggcc tggacggttg ccatcgcggt gatcgcgccg atcgtgctgt
    14221 tctgcgtggt gttcggggcg cgttcgacgg ggcgacggcg cgccgtgcta atcgcgatcg
    14281 cggtaggcgt gatgttcggg ctctccgcag tgctgaccaa actggtgatg ttcgagttgg
    14341 ccgaggacgg cgcagttgcg gtgctgaagt tgccgtcgct gtatctgctc gcggcggtgg
    14401 gtctgaccgc gacgttcttg cagcagtccg ccttccactc cggcgcgctg caaacgtcgg
    14461 tgccgacgat gctggtgctc gagccggtgg tcgcggtgct ggtgggcgcg ttcgtgctca
    14521 acgagcacct gtcggtgtcg ggctacgaga tggtggcgct gccgctcgca gtcggcgcga
    14581 tggccgcggc cacgatcgcg ctcggccgcg acgaaggcgc ctacgaggag caactggaag
    14641 ccgcgctggc cggtcgcgcc gaatcgtctt cgcggccttg agcttggcgc cgacgactga
    14701 ggtccgtttc gccgatttct acaccagggc tgtggaatcg ctgaaatcgc agccctggcg
    14761 cagaaatcgg cgcagccggg gtcagcgatt gatcgataaa ctgcccgaat caccgccaga
    14821 cgtacaccca acgccgagga acgccgccac gcgctgtgcg acgcggcgat ccaactgttg
    14881 gccgaccacg gcgcaaaggg cttgagccat cccaaggtcg accgtcaagc cggtgtacct
    14941 gaaggcagca cgtcgtacta ctttcgcact cgcaccgcat tgcttcgggc ggtggccgag
    15001 cggctggccg aactcgacct ggccgaactg aaatccgtca ccgaggcgac ccgtgtcgac
    15061 aacgggcgcc gatccgcccc gagggcctcc aggcttgcga ccgtcgtcat gaggtccgcg
    15121 accgaacccg gattgacccg cacgaaagcg cggttcgaac tgctgctcca agccaatcgg
    15181 gatcccgtgc tggctgacct tttccgccac aacacggaga tattcaccga gttgcatcgc
    15241 gagatcgtcg agcggtggca gccgcccggg gccgatgtcg attcctcggt gatcgacgat
    15301 cagacctatg tcacaatgaa tttcatcagc ggcgtcatga tgagtttggc cgcgggcgac
    15361 cgcacgat
//
LOCUS       DASWBF010000359         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_40480, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000359 DASWBF010000000
VERSION     DASWBF010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40480"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1932
                     /locus_tag="VF874_13325"
     CDS             337..1932
                     /locus_tag="VF874_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX9177006.1"
                     /translation="MFDSHGNVRGPYKGIYAELAPSDASELAARNEALGRAFINQGIT
                     FSLSGQERPFPLDLVPRVISAAEWSRLERGIRQRVRALEAYLDDIYGEQEILRDGVIP
                     RRLVTTCGQFHREAAGINPPNGVRIHVAGIDLVRDAEGMFRVLEDNLRSPSGVSYVME
                     NRRTMARVFPNLFATHRVRAVGDYSSHLLRALRNSAATNEADPTVVVLSPGVYNSAYF
                     EHSLLARQMGAELVEGRDLFCRDNQVYMRTTEGKRQVDVIYRRIDDDFLDPMQFRPDS
                     VLGVAGLLNAARAGNVVISSAVGNGVADDKLVYTYVPTIIEYYLGEKPLLANVDSYRC
                     WLDEEREEVLDRIAELVIKPVEGSGGYGIVFGPDASERELAVIAKKIRSDPRGWIAQP
                     VVQLSTVPTEIGDRLAPRHVDLRPFAVNDGDEVWVLPGGLTRVALLEGSLVVNSSQGG
                     GSKDTWVLASRASVADRELAAAQVVKSLPKIPTAAAGEKGPELRSTQQHSSRRPTAPS
                     NSNNNSSKPAGNSLGGSVKGSGW"
     gene            1940..2137
                     /locus_tag="VF874_13330"
     CDS             1940..2137
                     /locus_tag="VF874_13330"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX9177007.1"
                     /translation="MLARNAESLYWIGRYVERADDTAGILDVTVHQLLEDSSVDPDHT
                     SRVLLRVRASTPPTPSWTSGR"
     gene            2164..2919
                     /locus_tag="VF874_13335"
     CDS             2164..2919
                     /locus_tag="VF874_13335"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX9177008.1"
                     /translation="MPGGCSIVDSISAARENARSAREVTSTELWECLNTTYNALAERE
                     RAAKGLGPHEFFSFIEGRAAMFAGLADSTLSRDDGYRFMVLGRAIERVDMTVRLLLSR
                     VGDSASSPAWVTVLRSAGAHDTYLRTYRGVRDAGRVVEFILLDRLFPRSVFLLAAAGR
                     TKPRRARPPPAQPVGATAEAQRLLGRTRSELEFLRPGVLLETLEQRLAGLQRACRDIG
                     EAVALQYFHSAPWVAWVDAGRHNGALVIEDGEI"
     gene            2919..3083
                     /locus_tag="VF874_13340"
                     /pseudo
     CDS             2919..3083
                     /locus_tag="VF874_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
     gene            complement(3214..3339)
                     /locus_tag="VF874_13345"
                     /pseudo
     CDS             complement(3214..3339)
                     /locus_tag="VF874_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009819623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          559 a         1105 c         1109 g          566 t
ORIGIN      
        1 aggagtcgat tgacattcgg ttaccgatga cgtcgatcag cctctggggg aatggcagat
       61 ccatcaacgg cgtgggcagg tccaccagga tcggcacgtc gaccgacccg gagatgacgg
      121 cgcgttgttc cccgcccagc gtctagccgt ccgactggcc tgcgtaaacc gccgaggtgg
      181 ggcagcatat caatggtgag ccttcgcacg ttgccgcccg agaagccctc cgaggccacc
      241 cggccgcagc gcaacaatta ccacccggcg acgaagttcg agggcatctt cgagggctat
      301 cgtcgccgcg gttcctactc cgaggcgttc gacgagatgt tcgacagcca cggcaacgtc
      361 cgcggcccgt acaagggcat ctacgcggag cttgccccgt cggatgcgtc cgagcttgcc
      421 gcccgcaatg aggccctggg ccgcgcgttc atcaaccagg gcatcacgtt ctcgctgtcc
      481 ggccaggagc ggccgttccc gcttgatttg gtgcctcggg tgatctccgc ggccgaatgg
      541 tcgcggctcg aacgcggtat ccggcaacgg gtcagggcgc tggaggccta cctcgacgac
      601 atctacggcg aacaggagat cctgcgcgac ggagtcatcc cgcgccggct cgtcaccacc
      661 tgcggacaat ttcatcgcga agccgccggc atcaacccgc ccaacggcgt gcgtatccac
      721 gtcgccggga tcgacctggt ccgcgatgcc gagggaatgt tccgggtgct cgaggacaac
      781 ctgcgctcgc cgtcgggcgt gtcctatgtg atggagaacc gccggacgat ggccagggtt
      841 ttccccaacc tgttcgccac ccatcgggtg cgtgcggtcg gggactactc ctcgcatctg
      901 ctgcgtgcgc tgcgcaattc ggcggccacc aacgaggccg acccgaccgt ggtggtgctc
      961 agcccggggg tgtacaactc tgcctatttc gagcactcgc tgctcgcccg gcagatgggc
     1021 gctgaactgg tcgagggccg cgacctgttc tgccgcgaca accaggtcta catgcgcacc
     1081 acagaaggga agcgccaggt cgatgtcatc taccggcgca tcgacgatga ctttctcgat
     1141 ccgatgcagt tccgtcccga ctccgtgctg ggcgtggccg ggcttctcaa cgccgcgcgt
     1201 gccggcaacg tcgtgatctc cagtgcggtg ggcaacggcg tcgccgatga caagctggtc
     1261 tacacctacg tgccgacgat catcgagtac tacctcggcg agaagccgct gctggccaac
     1321 gtcgacagct accgttgctg gctggacgag gagcgcgaag aggtgctcga tcgcatcgct
     1381 gagctggtca tcaaaccagt cgaaggatcc ggggggtacg ggatcgtctt cgggccggat
     1441 gcctccgagc gcgaactcgc ggtcatcgcg aagaagatcc gcagcgaccc gcgtggctgg
     1501 atcgcccaac ccgtggtgca gttgtccacg gtgcccaccg agattggtga ccggctggca
     1561 ccgcggcacg tcgacctgcg gccgttcgcg gtcaacgacg gtgacgaggt ctgggtgctg
     1621 cccggtgggt tgacccgcgt cgcgctgctg gaaggctcgt tggtggtcaa ctccagccag
     1681 ggcggcggct ccaaggacac ctgggtgctg gcgtcgcggg cgtcggtggc cgaccgtgag
     1741 ctggccgccg cgcaggtggt caagtcgctg ccgaagatac ccaccgcggc cgcgggggag
     1801 aagggcccgg agctcaggtc cacccagcaa cacagcagtc gtcggccaac ggcacccagc
     1861 aacagcaaca acaacagcag caagccggcg ggcaacagtc tcggcggcag tgtcaagggc
     1921 agtggatggt gatggactga tgttggcccg caacgccgaa tcgctgtatt ggatcggccg
     1981 ctacgtcgag cgcgccgacg acaccgccgg catcctcgac gtcacggtgc atcagctgct
     2041 cgaagactct agcgtcgacc ccgatcacac gtcgcgggtg ctgttgcggg tgcgggcatc
     2101 gacccccccg acaccgagct ggacgtctgg tcgctgaccg acctggtcgc gttcagccgc
     2161 ggcctgcccg gcggctgctc gattgtcgac tccatctcgg ccgcgcgcga aaacgcaagg
     2221 tcggcaaggg aagtgacgtc cacggagctg tgggagtgtt tgaacaccac ctacaacgcg
     2281 ctggccgaac gtgaacgcgc ggccaaaggg ctgggcccgc acgagttttt ctcattcatc
     2341 gagggccgtg cggcgatgtt cgccggcctg gccgactcga cgttgagccg cgacgacggc
     2401 taccggttta tggtgctcgg tcgcgcgatc gagcgcgtcg acatgacggt gcggctgctg
     2461 ctgtctcgcg tcggcgacag cgcatcgtcg cccgcctggg tgacggtgct gcggtcggcc
     2521 ggtgcccacg acacctatct gcgcacctac cgcggcgtgc gcgacgccgg cagggtcgtc
     2581 gaattcatcc tgcttgaccg gctgttcccg cgatcggtct tcctactcgc tgcggctggc
     2641 cgaacaaagc ctcgaagagc tcgaccgccg cccgcacagc cggtgggagc caccgccgaa
     2701 gcacagcggc tgctcggccg cacccgcagc gagctggagt tcctgcgtcc cggcgtgctg
     2761 ctggaaaccc tggagcagcg attggccgga ctgcagaggg cctgccgcga tatcggagaa
     2821 gccgtggcgc tgcagtactt tcactccgca ccgtgggtgg cctgggtcga cgccggacga
     2881 cacaatgggg cgctggtcat cgaggacggg gagatctgat gtggcgcatg cgcgtcgtgc
     2941 attcgactgg ctacgcctac aagtcacctg ccaccgcgtc ctacaacgag gcgcacctga
     3001 caccgcgctc ggacaaccgc cagaacgtca tcctcagccg cgtcgaaacg gtcccgccca
     3061 cccggtcata tcgctacgtc gacggcactg tcaacttgag caatccgagg gcaattttcg
     3121 ccggtttgcc tccgcgattc accaggtcgc gtgtgacgta tgaggcttat gcggttagca
     3181 gaacgtccac acgacctcaa gttgacagtg cccccgctcg cgttgccggg tcggctggtg
     3241 ggagttctcg gcgcggttgt tcaggtactt cgaccggcga tgctgcaccg acggcacgag
     3301 ctcgcggtgc gccacctggt agctggcgag cttgtcggt
//
LOCUS       DASWBF010000360         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_40586, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000360 DASWBF010000000
VERSION     DASWBF010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40586"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..1836)
                     /locus_tag="VF874_13350"
     CDS             complement(325..1836)
                     /locus_tag="VF874_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9177009.1"
                     /translation="MTSLWLANRGQAPWVASTLDAQVQSADVVVAGAGITGLTTAVLL
                     ARAGKSVLVLEARTVGACATGNTTAKISLLQGTHLSKIIERHGVETAKAYVDGNREGQ
                     EWLVHRCEANGVAVQREDAYTFAQTGKGVPSARAELAACEAVGLNAKWNDDADVPFSY
                     HGGVRLADQAQFDPIPFLDSLVVELLERGGRLVERSRVRSVSSRGNGLKLRVNGPGDD
                     EVEVRAGQLVLATGIPILDRGGFFARLKPSRSYCLAFKVPGTITRPMMISTDSPTRSV
                     RYAPTAEGERLIVGGAGHPVGREKSPSKALEELSSWARVHYPGAVQTHFWSAQDYTPI
                     DQMPYVGPILPGNENIFVATGFNKWGMTNGAAAALALSSRILGGRMDWAKAFASWSPH
                     ELSGIPTALRANLEVGFNLVKGWITPLTRALHRQPSDDEGGVVSGPPWHLEARCVVGG
                     EEHRVSPVCTHLGGIVNWNDADKAWECPLHGSRFAPDGTLLEGPATRDLTRSQ"
     gene            1919..2149
                     /locus_tag="VF874_13355"
     CDS             1919..2149
                     /locus_tag="VF874_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177010.1"
                     /translation="MASEPTPDEPIDADVPLADAVEQQQEVDETPSLSENFEPTTVNE
                     IATEAAPDDANPADWQEQRTGAADGWDPDVDL"
BASE COUNT          437 a          867 c          771 g          385 t
ORIGIN      
        1 ccccgagccg gtagccatcg gccgtatagg aacgcgggac gcaggcgtgc ccgttgtctt
       61 cgacaaatcg ttctaggagg gcgctgaaca aggcggttct ggggctgccc gcccgcacgg
      121 gccagggcgc gagattcggc gtgcgggcgc acgcgaaggc ggattttggc ggggaagccc
      181 tagctagcgg cagtagatat cgatgcgacc cggacacaaa cgactcggtc cacgacccgt
      241 gcacatgcca caacaaaacc gtgcgcacct ggacggttgt cgccacgcca acaagtggtt
      301 cccggagcca cttgtggcca aacatcactg cgaccgggtc aagtcccggg tggccgggcc
      361 ctccagcagc gtgccgtcag gcgcgaagcg tgagccgtgc agcgggcact cccaggcctt
      421 gtccgcatcg ttccagttca cgatcccgcc aaggtgagtg cacaccggcg acacccggtg
      481 ctcctcgcca ccaacgacgc atcgggcctc gaggtgccac ggcggtccgc tgaccacccc
      541 gccctcgtcg tcactgggtt gccggtgcag cgcccgggtg agcggggtga tccagccctt
      601 cacgaggttg aagccgactt ccaggttcgc ccgcaacgcc gtggggatac cagacagctc
      661 atgcgggctc cagctggcga acgccttggc ccaatccatc cggccgccaa ggatccggct
      721 ggacaacgcc agcgccgcgg cagcaccgtt ggtcatcccc cacttgttga aacccgttgc
      781 gacaaagatg ttttcgtttc caggcagaat cggccccacg tacggcattt gatcgattgg
      841 tgtgtagtct tgggccgacc agaaatgcgt ctgcaccgct cccgggtagt gcacccgcgc
      901 ccacgacgat agttcctcca gcgccttcga ggggctcttc tcgcggccga ccgggtggcc
      961 ggcgccgccg acgatgagcc gctcaccctc ggcggtcggc gcataccgca ccgaccgagt
     1021 gggcgagtcg gtagagatca tcatcgggcg cgtgatggtg ccgggcacct tgaaggccag
     1081 gcaatacgac cggctcggct tgagccgcgc gaagaagccg ccacggtcga ggatcggaat
     1141 cccggtggcc agcaccagct gtccggcacg aacctcgacc tcgtcatccc cggggccgtt
     1201 gacacgcagc ttcagcccat tgccccgact cgacaccgac cgcacccgac tgcgctcgac
     1261 cagccggccg ccgcgttcga gcagctcgac taccagcgag tccaggaacg gtatggggtc
     1321 gaactgcgcc tggtcagcca gccgcacacc gccgtggtac gaaaacggca cgtcggcgtc
     1381 gtcgttccac ttggcattca gccccaccgc ctcgcaggcc gccaactcgg cgcgggccga
     1441 cggcaccccc ttgccggtct gcgcaaaggt gtaggcgtct tcacgctgca ccgctacccc
     1501 gttcgcctcg cagcggtgga ccagccattc ctgtccctcg cggttgccgt cgacgtaggc
     1561 cttcgccgtc tcgacaccgt gcctctcgat gatcttggac agatgggtgc cctgcaacag
     1621 gctgatcttg gcggtagtgt tgccggtcgc gcaagcgccg acggtgcgcg cctcgagcac
     1681 gagcacgctc ttgcccgcac gcgccagcaa caccgcggtc gtcaacccgg tgatgccggc
     1741 tccggcgacg acgacgtcgg cggactgcac ctgggcgtcg agggtgcttg cgacccaagg
     1801 agcttggccg cggttcgcta accacagtga agtcatgccg tgcgtcatac cctctgaccc
     1861 ggcgctgaaa ccgcgtgttc cgcgttttgc ctggcgcggg gcggggtacc ttcgatgcgt
     1921 ggcctccgaa ccgacacccg acgaaccgat cgacgcagac gttccgctcg ccgacgccgt
     1981 cgagcagcaa caagaagttg acgaaacgcc cagcctttcc gagaacttcg agccaacaac
     2041 ggtgaacgag atcgccaccg aggcagcacc cgacgacgcc aatcctgcgg actggcagga
     2101 gcagcgcacc ggtgccgccg acggctggga cccggacgtc gacctgtaga ggagcgaccg
     2161 tggccgacga ccagcaccac aagtcgggaa ccattccgta tcccggccac accgccgaca
     2221 tggcggagcg gccgcaagac gagatgcgcg actacgtcgg cggcactgtc aacttgagca
     2281 atccgagggc gattttcgcc ggttcgcctc cgcgattcac caggtcgcgt gtgacgtatg
     2341 aggcttatgt ggttagcaga acgtccacac gacctcaagt tgacagtgcc tctccgccgg
     2401 ataccggtgg cccttgcacg acacctcgcc cacgatgatc cctccggcgc atcgactccg
//
LOCUS       DASWBF010000361         8288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1286211,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000361 DASWBF010000000
VERSION     DASWBF010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286211"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..133
                     /gene="rrf"
                     /locus_tag="VF874_13360"
     rRNA            21..133
                     /gene="rrf"
                     /locus_tag="VF874_13360"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            272..1069
                     /locus_tag="VF874_13365"
     CDS             272..1069
                     /locus_tag="VF874_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9177011.1"
                     /translation="MVDDSARRGNKPPRTSGSGPRESWSGPGRARPRQPQAASDGDER
                     RRDEPALPPDIDAKQLSPEIRRELTTLDRVNADMVAKHLVAAGELLDDDPEAALAHAR
                     AARARSGRIAAVREAVGIAAYHCGDWAQALAELRAARRMGSKSSLLPLIVDCERGVGR
                     AERAIELARSPEAVQLTGDDADELRIVAAGARADLGQLDQALATLSTPPIDPARQGQT
                     AARLCYAYAETLLALGREDEAVQWFLRAAAADIDGVTDAEDRVAELA"
     gene            1071..2108
                     /locus_tag="VF874_13370"
     CDS             1071..2108
                     /locus_tag="VF874_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX9177012.1"
                     /translation="MTTLAGQHDCLLCDLDGTLFRGAEPTVGAIDTLAAVDARIFFVT
                     NNASRGAAEVAEHLREIGFTAKPDDVVTSAQTAAHLLASELAADSAVLVVGTDALAAE
                     VAAVGLRPVRRWSDSPVAVVQGHSPHTCWSDLAEAALAIRGGALWLAANVDVTLPTER
                     GLLPGNGAMVAALRAATEHEPRVAGKPQPTVFRDALGRGEFHSPLAIGDRLNTDIAGA
                     NAAELPSLMVLTGVSTAEDAVFAPMHERPTYIANDLRALGDAPDRLRVAPQSAWRVVA
                     DRAEITVTAAHDTAEDDGLSVLRAIADAVWGKSEHPARRQVVAGDDAAGEALRRAVAA
                     GVLKSDSVNPS"
     gene            2105..2281
                     /locus_tag="VF874_13375"
     CDS             2105..2281
                     /locus_tag="VF874_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177013.1"
                     /translation="MTADPEHIRAQIAGLLAELPDVTGDGVDLAALDIDAVAARLDEA
                     HDVLVRALESVEKG"
     gene            2288..3097
                     /locus_tag="VF874_13380"
     CDS             2288..3097
                     /locus_tag="VF874_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX9177014.1"
                     /translation="MARRARVDVELVRRGLARSRHQAAELIGAGRVSIDGMPAAKPAT
                     AVAVTAHLSVEDIGGRTWVSRGAHKLIGALDAFGIGAADRRCLDAGASTGGFTEVLLD
                     RGAREVVAADVGYGQLAWSLRSDPRVTVLERTNVRDLSTDTIGGPVDLVVADLSFISL
                     TTVLPALTSCASADADIVPMVKPQFEVGRERVGAGGVVTDPQLRAHAVLTVAERAAGL
                     GWPPVGVVASPLPGPSGNVEYFLHLRSQTDRPLTDETLKRAVTRAITEGPQ"
     gene            3094..4032
                     /locus_tag="VF874_13385"
     CDS             3094..4032
                     /locus_tag="VF874_13385"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX9177015.1"
                     /translation="MTSDRAVLLVVHTGRDEATDTARRVQKVLGDNDIALRVLSAEAV
                     DRGKLHLDPNEMRALDVDIEVVEPNHDAAVGCELVLVLGGDGTFLRAAELARNASVPV
                     LGVNLGRIGFLAESEAEAIDQVLEHVVTRDYRVEDRMTLDVSVRSDGEVIGQGWAINE
                     ASLEKGTHLGVLGVVLEVDGRPVSSFGCDGVLVSTPTGSTAYAFSAGGPILWPDLEAI
                     LVVPNNAHALFARPMVTSPKACIAIEAEAGGSDAVVVCDGRRQMPVPAGARLEVTRCA
                     TPVRWVRLDSAPFTDRLVRKFRLPVTGWRGAMTADG"
     gene            4033..5793
                     /gene="recN"
                     /locus_tag="VF874_13390"
     CDS             4033..5793
                     /gene="recN"
                     /locus_tag="VF874_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX9177016.1"
                     /translation="MLSEIRIESLGAIGSAVAEFDRGLTVLTGETGTGKTMVVTGLHL
                     LGGARAEASRVRSGADRAVVEGRFTTSDLDETAASHASEILESCGAERDDDGSVIASR
                     SVSSDGRSRAYLGGRSVPAKSLSTFTTELLTLHGQNDQLRLMRPDEQRAALDRYAGIG
                     AKLDRYRKLRNDWLQARRDLADRTDRARELVQEADRLKFAIEEITSVDPLPGEDNAIV
                     ADIQRLSELDALREAAQTARAALSGTHEETGDTDGSATDRIGQAKSALEGSDDGALVV
                     LSDQLGQALTLIADVTSELASYLDELPTDASALETKLARQAQLRTLTRKYAADVDGVL
                     AWAEQARERLTQLDISEQAVTALARTVDELAAQVAAAAVDLTKARTKAAKGLSKAVTS
                     ELAGLAMGDADFAITVQPMPARPDDSAPLIVPSGAAVLHAGSAGTDFVEFGFTAHKGA
                     AVLPVNKSASGGELSRLMLALEVVLAASAEGMTMVFDEVDAGVGGRAAVQIGRRLARL
                     ARTHQVIVVTHLPQVAAYADVHLVIDTDGRDGTSVVSRVDDDGRVGELARMLAGLGES
                     DAARAHARELLEKAAAERTS"
     gene            5949..7133
                     /gene="steA"
                     /locus_tag="VF874_13395"
     CDS             5949..7133
                     /gene="steA"
                     /locus_tag="VF874_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEX9177017.1"
                     /translation="MKMSSLLSRNTSSRPGLVGTVLVDRDIERLLRRVGPGDIVVLDA
                     RDLDRVTADALVDAGVTAVINASRSISGRYPNQGPEVLVANGVRLIDDTGPEVFKKVK
                     DGARVRVHNGAVYSGDRRLIVGAERSGEEISDLMHEAKSGLVEHLEAFAGNTIEFIRS
                     ESPLLIDGIGIPDIDVDLNRRHVVIVGEEDSAVEDIKSLKPFIKEYQPVLVGVGAGAD
                     VLRKAGYRPQLIVGDPDKMTADVLRCGAQVVLPADADGHAPGLERIQDLGVGAMTFPA
                     AGSAMDLALLLVDHHGAALIVTAGHTATIEEFFDRTRAQSNPSTFLTRLKVGQKLVDA
                     KAVATLYRSRISGGAIAMLVLAMLVAIIVALWVSRTDHAVLDWIAMYWNRFLIWAQGL
                     VS"
     gene            7145..8083
                     /locus_tag="VF874_13400"
     CDS             7145..8083
                     /locus_tag="VF874_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX9177018.1"
                     /translation="MISLRQHAISLAAVFVALAVGVVLGSGLLSDTLVSGLRDDKQQL
                     QDRISELDQHNNALTEKLSAAGEFDAEMAGRIVHGALNEKSVVIIRTPDATDDVVTSL
                     TRLVGDAGGTVTGAVTLTQQFVDANAADKLLSVVNSSIVPAGTQLNTTSVDQGAQAGD
                     LLGVVLQISKNPATPPVNDMQRETVLEALRQTGFITYSDKVAAANTAIVVTGGAMKED
                     AGNAGPTVARFATALAPHGAGTVLAGADGSASGTAAVAVVRADSGMAGAVTTVDDVDM
                     ESGRITAILALQDLLNGARTGQYGTGEGAQALTVPQ"
BASE COUNT         1437 a         2706 c         2808 g         1337 t
ORIGIN      
        1 aaacaacacc taaatacagt tacggcgaac atagcgacag ggaaacgccc ggacccatcc
       61 cgaacccgga agctaagcct gccagcgccg atgatactac ccaaccgggt ggaaaagtag
      121 gacaccgccg aacacacaca aaagaaatgc ccccgactac agccgggggc atttctttat
      181 ttcccgttat gaacacaaat ttcactgata ccgaagtgtc gataattcgg taatcgcgta
      241 tcctcgatac aaacacgtta ggaaggcaca agtggtcgac gacagcgccc gacgcggaaa
      301 caagccgcca cggacatccg gcagcgggcc gcgcgagtcg tggtccgggc cgggccgggc
      361 gcgcccccgt caaccccaag ccgcatcaga cggtgatgag cgccggcgtg acgagccggc
      421 actgcctccg gatatcgacg ccaaacagct gtcgcccgaa atacggcgcg agctcacaac
      481 tttggaccga gtcaacgccg acatggtcgc gaagcatttg gtcgcagcgg gtgagttgct
      541 tgacgacgat cccgaggccg cattggctca tgcccgagct gcccgggccc gctccggccg
      601 gattgccgcg gtccgcgagg ccgtcggcat cgcggcgtac cactgcggcg actgggcgca
      661 ggctctcgcc gaactgcgcg ccgcgcgccg catgggcagt aagtcgtcac tgctgccgct
      721 gatcgtcgac tgtgaacgcg gcgtcggacg ggccgagcgg gcgatcgagc tcgcacgcag
      781 tcccgaagcg gtccaactca ctggcgacga cgccgacgag ctgcgtatcg tcgcggccgg
      841 cgcccgcgcc gacctcgggc aactggatca ggccctggcc acgttgtcca ctccgccgat
      901 cgacccggcc cgccaagggc agacagccgc ccgactgtgc tacgcctacg ccgagacgct
      961 gctcgcgctg ggccgcgaag acgaagcggt gcagtggttc ctgcgcgccg cggcggccga
     1021 catcgacggg gtcaccgacg ccgaggaccg tgttgcagag ctcgcctgac atgacaactc
     1081 ttgccggcca acatgattgc cttctctgcg atctcgacgg cacgctcttt cgtggcgccg
     1141 aaccgaccgt cggtgctatc gacacgctcg cggcggtcga cgcccgcatc ttcttcgtca
     1201 cgaacaacgc ctcgagaggc gccgccgaag tcgccgaaca tcttcgcgag atcggattca
     1261 ccgcgaaacc cgacgatgtc gtcaccagcg cgcagaccgc cgcgcatctg ctcgcgtcgg
     1321 aactagcagc cgactctgcg gtgctcgtcg tcggcaccga cgccctcgcc gccgaagtgg
     1381 ccgcggtcgg cctacgcccg gtgcggcgct ggtccgattc gcctgttgca gtcgtccagg
     1441 ggcactcgcc gcacacctgt tggtccgatc tggccgaggc cgcgctcgcg atccggggcg
     1501 gcgccctgtg gctagccgcc aatgtcgacg tgacgctgcc gaccgaacga gggctgctgc
     1561 ccggcaacgg cgcaatggtc gcggctctgc gcgccgccac cgaacacgaa cccagagtcg
     1621 ccggcaaacc ccaacccacg gtcttccgag atgcgttggg ccgaggcgag tttcattcgc
     1681 cactggcgat aggcgacagg ctgaacacgg acatcgcggg tgccaatgcc gccgagctgc
     1741 ccagcctgat ggtcctcacc ggagtcagca cagccgaaga cgccgtgttc gcaccgatgc
     1801 acgagcggcc gacatacatt gcgaacgacc tccgcgcact tggggacgca cccgatcggc
     1861 tccgagtcgc ccctcaatcc gcctggcgtg tcgttgccga ccgcgccgaa ataaccgtca
     1921 ccgctgcgca tgacaccgcc gaggacgacg gcctgtcggt ccttcgtgcg atcgcggacg
     1981 ccgtgtgggg aaaatctgaa cacccggctc gccgccaagt cgtcgccggt gacgatgcgg
     2041 cgggcgaagc actgcgccgc gccgtcgctg ccggcgtcct gaaatcggat agcgtaaacc
     2101 cgtcatgacc gcagaccctg agcacatccg ggcgcagatc gccgggctgc tcgccgagct
     2161 tccagatgtc acgggcgacg gcgtcgatct ggccgcgctc gacatcgacg ccgtcgccgc
     2221 ccgcttggac gaggcacacg acgtgctcgt tcgtgccttg gagtcggtgg agaagggttg
     2281 acgagtcgtg gcccggcgag cgcgcgttga cgtcgaactg gtgaggcgcg gattggcccg
     2341 ctccaggcac caggccgcgg agctgatcgg cgcaggccgg gtcagcatcg acggaatgcc
     2401 ggccgccaag ccggcgaccg ccgtggcggt cactgctcac ttgagtgttg aggacatcgg
     2461 tggacggacc tgggtatccc gcggcgcgca caagctgatc ggtgcgctgg atgcattcgg
     2521 gatcggcgcg gcagaccggc gctgcctcga tgcaggcgcg tcgactggcg ggttcaccga
     2581 ggttctgctc gaccggggtg cgcgcgaagt tgtcgcggca gacgtcggct acggccagct
     2641 ggcctggtca ctgcgatcgg acccgcgggt gaccgtgctc gagcgcacca acgtgcgtga
     2701 cctctcgaca gacaccatcg gcggtccggt cgacctcgtg gttgcggatc tgtcgttcat
     2761 ctcgctcacg acggtgctgc ctgcgctgac gtcgtgcgca tcagcggacg cggatatcgt
     2821 tccgatggtg aagccgcagt ttgaggtcgg tagagaacga gtgggcgcgg gtggcgtggt
     2881 gaccgatccg cagctgcgcg cccacgcggt gctaaccgtc gctgagcgcg ccgccggact
     2941 cggctggcct ccggtgggcg tcgtcgcgag cccgctgccg gggccgtcgg gtaacgtcga
     3001 gtactttctg catctgcgct cacagaccga ccgtccgttg acggacgaga cattgaagcg
     3061 ggcggtgacg cgcgccatca cggaggggcc acagtgacca gcgatcgggc cgtgctcctc
     3121 gtggtgcaca caggccgcga cgaggccacc gacacagctc gtcgtgtcca aaaagtgctg
     3181 ggcgacaacg atattgcgct tagagtgctg tccgcagaag cggtcgaccg cggcaagctg
     3241 catctcgacc ccaacgaaat gcgcgctctc gacgtcgata tcgaggtcgt cgagcccaac
     3301 cacgacgcgg ccgttggctg cgagctggtg ctcgtcctcg gaggggatgg cacatttctg
     3361 cgtgccgccg agctggcccg gaatgcgtcg gtcccggtgt tgggcgtgaa tcttggccgc
     3421 atcgggtttc ttgccgaatc ggaggccgaa gccatcgacc aggtcctcga acacgtcgtc
     3481 acacgtgact accgcgtcga agaccgtatg acccttgatg tttcggtgcg ttccgatggt
     3541 gaggtcatcg gtcagggctg ggcgatcaac gaggccagcc tggagaaggg tacgcacctt
     3601 ggtgtgctcg gcgtcgttct cgaggtcgac ggacgaccgg tgtcgtcgtt cggctgcgac
     3661 ggcgtgctgg tgtcgacccc gaccgggtcg acggcctacg cattctctgc gggcgggccg
     3721 attttgtggc ctgacctcga agcgatcctg gtggtgccca acaacgctca tgcgttgttc
     3781 gcccgcccga tggtgaccag ccccaaggcg tgtatcgcga tcgaggcgga agccggtggc
     3841 agcgacgccg tggtggtgtg cgacgggcgg cgacaaatgc cggtgcccgc gggtgcgcgg
     3901 ctggaggtga cccggtgcgc gacgcccgtg cggtgggtgc gcctggacag cgcgccgttc
     3961 accgaccggc tggtgcggaa attccggctt cccgtcaccg gatggcgtgg cgcgatgacg
     4021 gcggacggct gagtgctctc ggaaatccgg atcgagtcgt tgggcgcgat cggctccgcg
     4081 gtcgcggagt tcgaccgcgg gctcaccgtg ctgaccggcg aaacaggcac gggaaagacg
     4141 atggtggtca ccggcctgca cctgctcggt ggcgcccgcg cggaagcctc ccgagtccgc
     4201 tcgggagccg accgcgcagt tgtggagggc cgcttcacca cctccgacct cgacgaaacc
     4261 gcggcctccc acgccagcga gattctcgag tcctgcggcg ccgagcgtga cgacgacggc
     4321 agcgtcatcg ccagccggtc ggtctccagc gacggtcgtt cgcgcgcgta tctcggcggc
     4381 cgcagtgtgc cggcgaagtc gctgagcacg ttcaccaccg aactactgac cttgcacggc
     4441 caaaacgacc aacttcggct gatgcggcct gacgagcagc gcgcggcact cgaccgctac
     4501 gccggcatcg gtgccaagct cgaccggtat cgcaaactgc gcaacgactg gctgcaggcc
     4561 cgacgtgatc tggccgaccg taccgaccgt gcgcgcgaac ttgtccagga agccgaccgc
     4621 ctgaagtttg cgatcgagga gatcacttcc gtcgacccgc tgccgggcga agacaacgcg
     4681 atcgtcgccg acatccaacg gctttcggaa ttggacgcgc ttcgagaggc cgcgcagacg
     4741 gcccgcgccg cgttgtcggg cacgcatgag gagaccggag acaccgacgg ttcggcgacc
     4801 gaccgcatcg gccaagcgaa gtcagcgttg gaaggcagcg acgacggcgc gttggtcgtg
     4861 ctttccgatc aactcggcca ggcgctgacg ttgatcgctg acgtgacaag cgagctggcc
     4921 tcgtatcttg acgaattgcc caccgacgca agcgctttgg agacaaagct ggcccgccag
     4981 gctcaattgc gaacgttgac ccgaaagtac gcggccgacg tcgacggtgt gctcgcctgg
     5041 gcggagcagg cccgcgagcg gctgactcaa ctcgacatct cagagcaggc ggtgaccgcg
     5101 ctggcgcgca ccgtggacga actggccgcc caggtcgccg cagcggccgt cgatctcacc
     5161 aaggcgcgca ccaaggcagc gaagggcttg tccaaggcag tcaccagcga gttggccggc
     5221 ctggccatgg gcgacgccga tttcgcgatc acggtgcagc cgatgccggc ccggccggac
     5281 gactccgcgc cactcatcgt gccgtcgggg gcggcagtcc tccacgccgg atcggcgggc
     5341 acggatttcg tggagttcgg tttcaccgcg cataagggcg cggctgtgct tccggtcaac
     5401 aagagcgcct ccggtggcga gctgtcaagg ttgatgcttg ccctcgaggt cgtgcttgcc
     5461 gcctcggcag aaggcatgac gatggtcttc gatgaggtcg acgcgggtgt cggcggacgg
     5521 gcggccgtgc agatcggacg gcggttggcg cggttggccc gcacccatca ggtgatcgtg
     5581 gtgacgcacc tcccacaggt cgcggcctat gccgacgttc acctggtgat cgacacggac
     5641 ggtcgcgacg ggaccagtgt ggtaagtcgc gtcgacgacg acggacgcgt cggcgagttg
     5701 gcgcgcatgc tggctggcct cggtgagtcc gacgccgccc gcgcacacgc ccgtgagttg
     5761 ttggagaaag cggccgccga acgcacctcc taaagcacaa gacgcaaaag cacccattcc
     5821 gtgccggaat aaggtgcttt tgcgtctgct cgtggaaaaa ccgcgggtgt gacgcgagtt
     5881 aacttgtgtg gctattgtta cggcgcgcct ccccggcatc ggcatgattc gtagccagaa
     5941 tcgccgctat gaagatgtca tcgctgctgt ctcgaaatac gtcctcgcga cccggactcg
     6001 tcggaaccgt attggtggat cgtgacatcg aacggttgct gcgtcgcgtc ggcccagggg
     6061 acatcgtcgt actcgatgct cgcgatctcg accgggtgac cgccgacgcg cttgtcgacg
     6121 caggagtcac agcggtcatc aacgcgtcgc ggtccatttc gggccgctac ccgaaccaag
     6181 gccccgaggt cctggtggcc aacggcgtga ggctgatcga cgacaccggg ccggaagtct
     6241 tcaagaaggt caaggacggc gcgcgagtgc gggtgcacaa cggcgccgtc tattccgggg
     6301 atcggcgcct gatcgtcggc gcggaacgca gcggcgaaga gatctccgat ttgatgcacg
     6361 aggccaagag cgggctcgtc gagcatctgg aggcgttcgc cggcaacaca attgagttca
     6421 tccgcagcga aagcccattg ctcatcgacg gaatcggcat tccggacatc gacgtcgact
     6481 tgaaccgccg ccacgtcgtg atcgtcggcg aagaggactc ggcggtcgag gacatcaaga
     6541 gcctgaaacc gttcatcaag gagtaccagc cggtgctggt cggcgtcggc gcgggcgcgg
     6601 atgtactacg caaagctgga tatcgcccgc agctgatcgt cggcgaccct gacaagatga
     6661 ctgccgacgt gctgcgttgc ggcgcgcagg tggtgctgcc ggccgacgcc gacggccacg
     6721 cacccggttt ggaacggatc caggacctcg gcgtcggcgc gatgaccttc ccggccgccg
     6781 gctctgccat ggatcttgcg ttgctgctgg tagatcacca cggtgcggcg ctgatcgtca
     6841 ccgccgggca caccgcgacg atcgaagagt tttttgatcg cacccgcgcg cagagcaacc
     6901 cgtcgacgtt tctgacgcgc ctgaaagtgg gacagaagct ggtcgacgcg aaggctgtcg
     6961 ccaccctgta ccgcagcagg atctccgggg gcgcgatcgc gatgcttgtc ttggcaatgt
     7021 tggtcgcaat catcgttgcg ctgtgggtgt ctcgcaccga ccacgcggtg ctggactgga
     7081 tcgccatgta ctggaaccga ttcctgatat gggcccaggg cctggtcagc taggacacgc
     7141 gttcgtgatt tcgcttcgtc agcacgcgat ttccctggcc gccgtcttcg ttgcgctggc
     7201 ggtgggagtg gtgctgggat cggggctgct gtcggacacg ttggtctcgg gcttgcgaga
     7261 cgacaagcag cagctgcagg acaggatcag cgagcttgac cagcacaaca acgcgctgac
     7321 cgaaaagctg agcgcggcag gcgaattcga tgccgagatg gccggacgga tcgtccatgg
     7381 cgcattaaac gagaaatcgg tggtgatcat ccgcaccccg gacgcgacgg acgatgtcgt
     7441 gacgtcgctg acgaggttgg tgggcgacgc gggcgggaca gtgaccggag cggtgacgtt
     7501 gacgcagcag tttgtcgatg ccaacgccgc cgacaaactg ctctcggtcg tgaactcctc
     7561 aatcgttccg gccggcaccc agctgaacac gacttcggtc gaccagggcg cccaggccgg
     7621 cgacctgctc ggcgtggtcc tgcagatcag caagaatccg gccacgcccc ccgtcaacga
     7681 catgcagcgg gaaacggtgc tggaagcgct gcgccagacg gggttcatca cgtactccga
     7741 caaagtcgcg gcggcgaaca ccgcgatcgt ggtgaccggc ggcgccatga aagaggacgc
     7801 cggcaacgcc gggcccaccg tcgcacggtt cgcgacggcg ctggcgccac acggcgcggg
     7861 cacggtgctc gccggcgcag acgggtcggc gtcgggcacc gcggcggtcg cggtggtgcg
     7921 tgccgactcc ggaatggccg gtgccgtcac caccgtggac gacgtcgaca tggagtccgg
     7981 gcggatcacc gcgattctgg cccttcagga cctgctcaac ggagcacgca ccggtcagta
     8041 cggcaccggt gaaggcgcgc aggcactgac cgtcccgcag tagcgcaatc gctcatcgcc
     8101 ggcctccggg cgtgttaacg gcgccttgtc ggccgcggtg ttaaggtgaa attccgtggg
     8161 tcggcaggcc ccagctattg aaagcattgc cctgcgccgt cgtcacggga gtcactcttg
     8221 ccagcgttac gcaagcaccc gcagaccgct acgaagcatc tgttcgtcag tggcggcgtg
     8281 gcatcgtc
//
LOCUS       DASWBF010000362        30748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1286285,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000362 DASWBF010000000
VERSION     DASWBF010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 30748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30748
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286285"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF874_13405"
     CDS             complement(<1..279)
                     /locus_tag="VF874_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9177019.1"
                     /translation="MRIALAQIRAGTDPAANLKLVADDTRRAADAGAVLVVFPEATMC
                     RLGVPLAPVAEPLHGPWASAVRSIAADAGVVVVAGMFVPSGDGRVTNTL"
     gene            complement(299..814)
                     /locus_tag="VF874_13410"
     CDS             complement(299..814)
                     /locus_tag="VF874_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX9177020.1"
                     /translation="MGRRLDYTVTYDAPAEEIYRSFTSRHYWESLLEEYRNLTPQSEI
                     TRFSSDHTGTRVVFTHNLPRAYLPPIARAVMPKDVIITREQHFEPYDRDRNRAGGTYG
                     ASIPAVVAHFRGSHLLTKTDQGCQLRLASVCKVAIPLVGGRLEQLVIRHITELFDAEE
                     KFTTDWVSKHH"
     gene            complement(836..1342)
                     /locus_tag="VF874_13415"
     CDS             complement(836..1342)
                     /locus_tag="VF874_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX9177021.1"
                     /translation="MPRSFDLTANYHGTVDQVHSAFSDEEYWLARLADSGADIATLNS
                     IVVGGDGTVTVATTQTLRTDRLPGMVTQFHPRDLQIVRNETWSPLSDGVAHAEVSGAV
                     PGAPVSLAGTAVLAPKDGRSRLKFTATVEVDIPLVGGKLESFIGGKLAGLVVAEQRFT
                     TVWIVENI"
     gene            1402..2463
                     /locus_tag="VF874_13420"
     CDS             1402..2463
                     /locus_tag="VF874_13420"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX9177022.1"
                     /translation="MSSVAEYAGARVAENVPLASLTTLRVGPVARRVITCDTTDQVVA
                     TLRALNSQPGPVLVMAGGSNVVVADKMTDLTVVKLATTGVTIDGATLRVEAGAEWDVV
                     VAESLRHGLGGLECLSGIPGSAGATPVQNVGAYGVEVADTITGVRLLDRGSGDVRWVP
                     PEELGFGYRRSVLKNTTSSVVLEVEFRLDASGRSAPLRYRELITALHAREGERTDPVR
                     VRDAVLALRRSKGMVLDGADHDTWSVGSFFMNPVVAQSDYQRLAQRSDGPVPHFPAPD
                     GVKLAAAWLVEQAGFGKGFPGPQAPARLSTKHSLALTNRGSATAADVVALARTVRDGV
                     RDKFGITLIPEPVLVGVTI"
     gene            2498..3781
                     /locus_tag="VF874_13425"
     CDS             2498..3781
                     /locus_tag="VF874_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9177023.1"
                     /translation="MTSANDAPRFTRRRALTALAVGVVAPGVLASCLSKSGKQDEAAG
                     PPPKPTVTLRPGNGTKDVAPTAAIGAEVRDGWFQRVTVTNADGKIVAGALSRDRTSYS
                     VTEPLGYGMKYTWAGSVVGRDGIAVPVNGSFSTVDPTSTVNGQFQLADGQTVGIAVPI
                     ILQFDASISDKAAVERALVVTTEPATEGSWAWLPDEAEGSRVHWRTREYYQPGTTVHV
                     DAKLYGVNFGEDAYGAEDVSLNFTIGRRQIVKANAQSHRIQVETDGGVIMDFACSYGE
                     ADQPRNVTRSGTHVVSEKYADFYMSNPAAGYSNVHERYAVRISNNGEFIHCNPESIGA
                     QGNTNVTNGCINLNLDNAIEYFNSAIYGDPVEVTGTSIQLSYSDGDIWDWAVDWDTWR
                     SMSAITPEQPPASIPSTAPVTPPGAPKPVSGRPGG"
     gene            complement(3759..4505)
                     /locus_tag="VF874_13430"
     CDS             complement(3759..4505)
                     /locus_tag="VF874_13430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177024.1"
                     /translation="MSAEARVAVVTGASSGIGAATAKTLAEQGFHVVAVARRAERIEA
                     LAREIGGTAVVADVTDDSAVDALAARLDRVDVLVNNAGGARGLAPVADADLEHWRWMW
                     ETNVLGTLRLTRALLPKLIDSGDGLIVTVTSVAALETYDGGAGYTAAKHAQGALHRTL
                     RGELLGKPVRLTEIAPGAVETEFSLVRFDGDQDRADAVYRGITPLVADDVAEVIGFVA
                     SRPSHVNLDLVVIRPRDQATASRFSRQDGR"
     gene            4734..5972
                     /locus_tag="VF874_13435"
     CDS             4734..5972
                     /locus_tag="VF874_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9177025.1"
                     /translation="MRSTIFLTRHNVVAPVLSLPEAAAAAVFGAARTRGPIARDAVAQ
                     VTSLSIATVNRQVTALLDAGLLRERPDLAASGAIGRPRVPVEVNHEPYLTLGLHIGAR
                     TTSIVATDLLGRTLDVVETPTPQTGQVTALPALAASARRYLSRWHRRRPLWVGVAMGG
                     VVDGATGTVDHLRLGWIDAPVGPVLAETLGLPVSVAVHVDAMAAAELLLGMRRFAPAS
                     GRYPHGAESTSLYIYARETVGYALVIDGRVHSPATGPGNIAALPAHSELLGGSGRLES
                     TVSDEAVLDAARRLSMSGSGTLAAVLRAARAGSGPAQELLAERARVLGSAVALLRDML
                     NPDDLVVGGQAFTEYPEGMSAVEAAYAENSTLPPRDIRVAAFGNRVQEAGAGVVSLGG
                     LYADPIGAMRRAVRRVGVSA"
     gene            6053..7375
                     /gene="mshA"
                     /locus_tag="VF874_13440"
     CDS             6053..7375
                     /gene="mshA"
                     /locus_tag="VF874_13440"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HEX9177026.1"
                     /translation="MTDVSVPRRVAVLSVHTSPLAQPGTGDAGGMNVYILQSALNMAG
                     RGVEVEIFTRATSSADPPTVRVAPGVLVRNVVAGPFEGLDKHDLPTQLCAFTAGVLRA
                     EATHEPGYYDIVHSHYWLSGQVGWLTRDRWAVPLVHTAHTLAAVKNAALADVDTPEPP
                     MRAVGEQQVVDEADRLIVNTEDEARQLISLHSADPERIDVVHPGVDLQLFTPGDKTAA
                     RAALGLGDDEQVVAFVGRIQPLKAPDILLRAAAKLTGRYPRLRVLVAGGPSGSGLEAP
                     DALMRLAVELGIGTRVTFLPPQSPSELVTVYRAADLVAVPSHSESFGLVAIEAQACGT
                     PVVAAEVGGLPVAVRDGVSGALVAGHDVDDWAETIGAMLDRGGPAMTRAAVAHAANFS
                     WARTVDALLAAYGRAIADYSTSRQRSPVVTLASRRNGRRWALRRGIRA"
     gene            7372..7914
                     /locus_tag="VF874_13445"
     CDS             7372..7914
                     /locus_tag="VF874_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX9177027.1"
                     /translation="MNVQKVIEDALDEHSLPYFHHDGAHGGPPDIIVELPGERRLKTT
                     TILSIGEHSVRVEAFVCRKPDENHETVYGFLLKRNRRLYGVAYTLDNLGDIYLVGRMA
                     LSSVTADEIDRVLGQVLEAVDQDFNTLLELGFSTSIRREWAWRVSRGESLKNLEAFAH
                     LVDERSREEHVIDKDAPSAP"
     gene            7927..8688
                     /locus_tag="VF874_13450"
     CDS             7927..8688
                     /locus_tag="VF874_13450"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HEX9177028.1"
                     /translation="MGDVGTLVLLRHGESEWNAKNLFTGWVDVDLTEKGRAEAIRGGE
                     LIAEQNLHPDVLYTSLLRRAITTAHLALDEADRLWIPVERSWRLNERHYGALQGLNKA
                     ETKERYGEEQFMKWRRSYDTPPPPIERGSTFSQDADPRYVNIDGGPLTECLADVVARF
                     LPYYTDVIVPDLRLGKTVLIAAHGNSLRALVKYLDDMSDDDVVGLNIPTGIPLRYDLD
                     ENLRPVVPGGQYLDPEAAAAGAAAVASQGADARSK"
     gene            8809..9975
                     /locus_tag="VF874_13455"
     CDS             8809..9975
                     /locus_tag="VF874_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9177029.1"
                     /translation="MTVASAVLVAAVAALAALIFGLAVGAGVMPRIAERNRRIVAREA
                     GLTVSQMLQRIVALAPTGMAVVDTHRDVVFMNARAQELGLVRDRLLDDRAWAAARRVL
                     ASGEDVDIDLSAGRSPSGRSGFSVRGRVRLLAEEDRRFAVIYVFDDSEVARMEATRRD
                     FVANVSHELKTPVGAMGLLAEALLESADDPETVRRFGEKMHLESQRLANMVNELIELS
                     RLQGAERLPELEAVDVDTVVAEALSRSKVAADNAYISIATDKPSGFEVMGDQALLVTA
                     LANLVSNAISYSPPGSPVSISRRRRGGNVEIAVTDRGIGIAREHQQRVFERFFRVDKA
                     RSRATGGTGLGLAIVKHIAANHNGTIRLWSQPGTGSTFTLSIPAYEDAGDESDG"
     gene            9992..10678
                     /gene="regX"
                     /locus_tag="VF874_13460"
     CDS             9992..10678
                     /gene="regX"
                     /locus_tag="VF874_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensory transduction protein RegX"
                     /protein_id="HEX9177030.1"
                     /translation="MTSVLIVEDEESLADPLAFLLRKEGFEATVVADGPTALAEFDRG
                     GADIVLLDLMLPGMSGTDVCKQLRARSSVPVIMVTARDSEIDKVVGLELGADDYVTKP
                     YSARELIARIRAVLRRGTDADDFGFDDGVLESGPVRMDVERHVVSVNGEPITLPLKEF
                     DLLEYLMRNSGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRSKIEADPANPVHLVT
                     VRGLGYKLEG"
     gene            complement(10660..11157)
                     /locus_tag="VF874_13465"
     CDS             complement(10660..11157)
                     /locus_tag="VF874_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177031.1"
                     /translation="MRILRQRDGVSCGPAVAVVAGARLDPDYAAVLADDRRFAEEQLR
                     VHAAVNRVWPRRLGLTPAGMAHALSAHRPYRWRLFRGRRDTLADVATAVASGAPVAML
                     IGNVIPRHWVLLVGVAGYEFCCYEPSSGDTLTCSFDAIRRSRLEGLGFPRPFAFALPT
                     YPSSL"
     gene            complement(11154..11453)
                     /locus_tag="VF874_13470"
     CDS             complement(11154..11453)
                     /locus_tag="VF874_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9177032.1"
                     /translation="MSDKLFLFRLLPPRADFAQTMTPEEQDAMARHAAYWRGLLDAGR
                     VVVYGPVADPEGVWGLGVVRAGSQAEVVELGEQDPVIVAGVASFDVAEIMGGITG"
     gene            complement(11450..12259)
                     /locus_tag="VF874_13475"
     CDS             complement(11450..12259)
                     /locus_tag="VF874_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177033.1"
                     /translation="MRLPLSSVSAPHPGEVELDFAREWMEFYDPDNPEHLIAADMTWL
                     LSNWTCVFGTPACRGTVEGRPDDGCCSHGAFLTDDDDRAQLDDAVKKLTAKDWQFREK
                     GLGRKGYLERDEYDGEKSWRTRKYKGACIFLNRPGFSGGIGCALHIKAMRLGVEPLTM
                     KPEVCWQLPIRRSQEWVTRPDGSEILKTTITEFDRRGWGEGGADLHWYCSGDPAVHVG
                     RKQVWESLAAELTELLGEKAYAELAAMCKRRKGLGLIAVHPATRAAERPSK"
     gene            12426..12749
                     /locus_tag="VF874_13480"
     CDS             12426..12749
                     /locus_tag="VF874_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177034.1"
                     /translation="MKSTTPEGSPIPPAERWIVMLVDTNLEWTRNRRETQPTNREYIM
                     STIINRKSVTAALGATVAAATVPALLVLGAGTAQAGTSAWTTTDALGVTVRARGSRLP
                     HGFQV"
     gene            13069..14073
                     /locus_tag="VF874_13485"
     CDS             13069..14073
                     /locus_tag="VF874_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX9177035.1"
                     /translation="MRLGVLDVGSNTVHLLVVDARRGGHPTPMSSTKASLRLAEAIDD
                     SGKLTRKGADKLISTVDEFAKIAASSGCAELMAFATSAVRDAKNSEDVLARVLAETGV
                     ALQVLSGVDEARLTFLAVRRWYGWSAGRIINIDIGGGSLELSSGVDEEPDVAMSLPLG
                     AGRLTREWLPDDPPGRRRVAMLRDWLDTELADASRTLLKAGPPDLAVASSKTVRSLAR
                     LTGAAPSAAGPRVKRTLTATGLRQLIAFISRMTVADRAELEGVSAERSPQIVAGALVA
                     EASMKALSIETLDICPWALREGLILRKLDSEADGTALVETSVGDAGHVGAKRRAGNSR
                     "
     gene            14070..14942
                     /locus_tag="VF874_13490"
     CDS             14070..14942
                     /locus_tag="VF874_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177036.1"
                     /translation="MTGPHDNSDTRPISVAELLARSGVIGAPPVGGRRRKRRGNADAV
                     TVAELTGEIPIIRDEEPAVEQAPAETNGTVDTVAEPEPEVQPEVDQEVEPVDVPAVAE
                     DLADADVPAEEMLPDHVDIDFDEDTDDHDAAAAVDEDLDETDETGEPPSYLDAVQEPL
                     FGGRTDDDLVRDDETAQSVTELPDVPPETTTAEDHEEVAPPTRLQQIGRGALVVLQSI
                     LAVAFGAGLFIAFDELWKWNNIVALVLSVLVILGLVVGVRIVRKTEDIASTLIAVAVG
                     ALVTLGPLALLQSG"
     gene            14959..15804
                     /locus_tag="VF874_13495"
     CDS             14959..15804
                     /locus_tag="VF874_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX9177037.1"
                     /translation="MRPAIKVGLSTASVYPLRTEAAFEYAARLGYDGVELMVWAESVS
                     QDVDEINKLSQHYGMPVLAVHAPCLLISQRVWGANPIPKLNRTVRAAEKLGAQTVVIH
                     PPFRWQRRYADGFTEQVARLEASSDVIVAVENMFPFRADRFFGGTQASIERMRKRGGR
                     PGPAISAFSPSYDPLEGNHAHYTLDLSHTATAGTDALDMARRMGSGLTHLHLADGSGA
                     YLDEHLVPGRGTQPTFEVCQMLAASDFAGHVILEVTTSGARTEAEREVLLTESLEFAR
                     THLLR"
     gene            15814..16662
                     /locus_tag="VF874_13500"
     CDS             15814..16662
                     /locus_tag="VF874_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9177038.1"
                     /translation="MTEASLFSNAMALRPLGDGVFAGELNKIWTIGPKLHGGAMLSLC
                     AVAAREAVGGVVQPISVSANYLSAPSPGDLRLETFVRKRGRRISVVDVELIGGDRVGD
                     ERSREEQKTAVHAVVTLGEPEHLAPPLLSANPVVSLMSPEPPPDVEPIGPGHPMFGTV
                     NYWEGIDVRPLMSTVPDGPSADGRTPVIQIWARPRDMAPDILFALMCGDVSMPVAFAV
                     GRRGWAPTVQLTAYLRGLPADGWLRVMATTTQIGQDWFDEDHLVVDSVGRIIVQTRQL
                     ALVPDH"
     gene            16684..17538
                     /gene="proC"
                     /locus_tag="VF874_13505"
     CDS             16684..17538
                     /gene="proC"
                     /locus_tag="VF874_13505"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX9177039.1"
                     /translation="MSRIAIIGGGNIGEALLSGLLRSGRQVKDIVVAERVPARTRHLS
                     ETYSVLVTSVADAVENANFVVVAVKPSDVESVTDEIAAAVARDDAGSIEHVFVTVVAG
                     VSTTYFEGKLPAGAPVIRVMPNAPMVVGAGVSAIAKGRFATPEQLKDVAALFDSVGGV
                     LKVPESQLDAVTAVSGSGPAYFFLMVEALIDAGVAVGLSRADATDLVVQTMAGSAAML
                     LERVDENGGSGDIDTTPARLRAAVTSPAGTTAAGLAELERGGLRAAVAAAVVAAKNRA
                     GQLGVTSE"
     gene            17678..17938
                     /locus_tag="VF874_13510"
     CDS             17678..17938
                     /locus_tag="VF874_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9177040.1"
                     /translation="MTSTNGPSGRDSASGKRDSGAADVQPAARAQFLTVAEVASLMRV
                     SKMTVYRLVHNGELPAVRVGRSFRVHAKAVHDLLETSYFDVS"
     gene            18102..18203
                     /locus_tag="VF874_13515"
     CDS             18102..18203
                     /locus_tag="VF874_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEX9177041.1"
                     /translation="MGSVIKKRRKRMSKKKHRKLLRRTRVQRRKLGK"
     gene            18268..19332
                     /locus_tag="VF874_13520"
     CDS             18268..19332
                     /locus_tag="VF874_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9177042.1"
                     /translation="MDSAGSDTFQSRKVVLVTGACRFLGGYLTARLVQNPVIERVIAV
                     DAIAPSKDLLRRMGRAEFVRADIRNPFIAKVIRNNDVDTVVHAAAASYAPRAGWRAAL
                     KELNVMGAMQLLAACQKAPAVRRVVLKSTSEVYGSSTRDPVVFTEDCTSRRPLGSGFA
                     QDSLDIEGYARGLGRRRPDIAVTILRLANLIGPAMDTALSRYLAGPFVPTVLGRDARL
                     QLLHEQDALGALERGTMAGRAGTFNVGADGIIMMSQAIRRAGRIALPVPGFGVWAVDS
                     LRRLNNYTEINREQLNYLSFGRVMDTTRMKVELDYHPKWSTAEAFDDYVRGRGLTPII
                     DPRWVRSLERRAVATAQRWG"
     gene            19376..20440
                     /locus_tag="VF874_13525"
     CDS             19376..20440
                     /locus_tag="VF874_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9177043.1"
                     /translation="MSGEPKAKVIPLHGDSGPAQRRAARRAEAASRHPSLLSNPDSSG
                     SAEDIAAVVREIDAHRSTAASGAPEQPEEPNELARRIGAVAEFMRRRFTGDYDVDEFG
                     FDPHFNDAVVMPVLRTLFASWFRVDVTGVENLPETGAALVVANHAGVLPFDGLMASVA
                     VHDKHPAQRTLRLLAADMVLDSPLLGQAARKAGHTMACTADAHRLLSKGELTAVFPEG
                     YKGLGKRFKDRYKLQRFGRGGFVSAALRTRAPIIPCSIVGSEEIYPMIGDVRLLARLF
                     GLPYFPITPLFPLAGPIGMIPLPSKWHIEFGEPIPTADYDDAAPEDPMITFELTDQVR
                     ETIQQTLYRLLANRRNTFFG"
     gene            complement(20405..20971)
                     /locus_tag="VF874_13530"
     CDS             complement(20405..20971)
                     /locus_tag="VF874_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9177044.1"
                     /translation="MAIAEEIIGTHYRYPDYFAVDREKIREFARAVQDDHPAHFSEDA
                     AAELGYPALVASLTFLTVAGRKVQLEIFDQFDVPINMERVLHRDQKLKFHRPILAGDK
                     LFFDSYLDSVIESHGTVITEIRGEVTDADGQPVITSVVTVIGEAQYDDEADEVVSAIA
                     AGRDAAIAKALGKARADSAEEGVPPVGE"
     gene            complement(21019..21918)
                     /locus_tag="VF874_13535"
     CDS             complement(21019..21918)
                     /locus_tag="VF874_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX9177045.1"
                     /translation="MTDPDSQGIAGDASAEAAVAHLAADVARNAELPQPPRPGAAPPP
                     PVDLTAAAFFDVDNTLVHGSSLVLFARGLAARKYFNYSDLIRFVYAQAKFQLTGRENS
                     DDVAAGRRKALAFIEGRSTAELVAVGEEIYDDIIADKIWPGTRALAQMHLDAGQQVWL
                     VTATPCELASTIAKKLGLTGALGTVAESVDGVFTGLLVGDILHGSGKAHAVRSLAIRE
                     GLNLKRCTAYSDSYNDVPMLSLVGTAVAINPDSALRELARHRGWEIRDFRTARKAARI
                     GVPSALALGAAGGALAAIASRRR"
     gene            21957..22214
                     /locus_tag="VF874_13540"
     CDS             21957..22214
                     /locus_tag="VF874_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX9177046.1"
                     /translation="MNRRVTLLTRDGCATCEQARLRLCELAGELGFDLSVTDVDAEAA
                     DGRPGSRAEFGDRLPVVLLDGREHSYWEVDEPRLRADLLRN"
     gene            22342..23679
                     /locus_tag="VF874_13545"
     CDS             22342..23679
                     /locus_tag="VF874_13545"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX9177047.1"
                     /translation="MSVLLFGVSHRSAPVQVLEQLSSGESDQVKIADRLLQSSLITEA
                     MVLSTCNRVEVYAVVDAFHGGLSVIGQVLAEHSGMSLNDLTKYAYVRYAEAAVEHLFA
                     VASGLDSAVVGEQQVLGQVRRAYAAAEANRTVGRVMHELAQRALSVGKRVHTETSIDA
                     AGASVVSVALGMAETRLDTLAGRTAVVVGAGAMGALSAKQLAHAGVERVHVVNRSLPR
                     ARRLAHNIREVGVRADASALDHLPNVLAEADVVVCCTGAVRPVVSLADVHRGLAHGAE
                     PRRLVICDLGMPRDVDAAVAGLPGVFVVDMERIQREPSARAAAADAEAARNIVAAEVA
                     QYLSAQRMAEVTPTVTALRQRAADVVETELLRLDNRLPDLAGAHREEVARTVRRVVDK
                     LLHAPTVRVKQLASAPGGDSYAEALRELFELDQQAVDAVAAGELPLAIDPESG"
     gene            23689..24609
                     /gene="hemC"
                     /locus_tag="VF874_13550"
     CDS             23689..24609
                     /gene="hemC"
                     /locus_tag="VF874_13550"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX9177048.1"
                     /translation="MRIGTRGSLLATTQAGVIRDALIAKGHAAELVTISTEGDRSAEP
                     IATIGVGVFTAALREAIDDGIVDMAVHSYKDLPTAEDKRFTIAAVPRRGDPRDALVAR
                     DGLVLGELPTGSVIGTSSPRRAAQLRALGLGLEIRPLRGNLDTRLNRVMSGDLDGIVV
                     ARAGLARIGRLDDVTETLEPVQMLPAPAQGALAVECRSADTKLAALLAELDDADTRAA
                     VTAERVLLAELEAGCSAPVGAIAEVVESIDEDGRVFEELSLRGCVATVDGSDVIRASG
                     IGTPGKAAELGRSVAAELFDLGARELMGTR"
     gene            24612..26264
                     /locus_tag="VF874_13555"
     CDS             24612..26264
                     /locus_tag="VF874_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional uroporphyrinogen-III
                     C-methyltransferase/uroporphyrinogen-III synthase"
                     /protein_id="HEX9177049.1"
                     /translation="MTRGRKARPGRVVFVGSGPGDPGLLTGRARSVLSCAELVFTDPD
                     VPAEVLAVVGSSLPDPPKLSSPDPGITDDTVEPNPPLVPDIRPALGDPAEVAKILAAE
                     ARAGADVVRLVAGDPLSVDAVITEVQAIAKAHLLFEIVPGLPASTAIPTYAGLPLGSS
                     HTVADVRGDVDWAALAAAPGPLILHATAEHLADAARTLIDYGIDDGTPCVVTANGTTT
                     QQRSVEGTLQTLTDSAALGGSDPAGPSPAGAWALTGPLIVTIGRTVASRAKLNWWESR
                     ALYGWTVLVPRTKDQAGDMSERLITHGAVPIEVPTIAVEPPRSPAQMERAVKGLVDGR
                     YQWVVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGQATADRVRAFGITPELVPSG
                     EQSSLGLLDEFPPYDDVFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRA
                     APPPAGTREMIKSGGFDAVCFTSSSTVRNLVGIAGKPHARTIVACIGPKTAETAAEFG
                     LRVDVQPEVAQIGPLVDALAEHAARLRAEGALPPPRKKSRRR"
     gene            26267..27247
                     /gene="hemB"
                     /locus_tag="VF874_13560"
     CDS             26267..27247
                     /gene="hemB"
                     /locus_tag="VF874_13560"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX9177050.1"
                     /translation="MGFPRQRPRRLRSTPALRRLVAQTSLEPRHLVLPMFVADGIDEP
                     RPIASMPGVVQHTHDSLRKAAADAVAAGVGGIMLFGVPREEDKDPTGEAGVTIDGVLN
                     RALQELNKDLGSATVLMADTCLDEFTDHGHCGLVDFSGRVDNDLTLSRYVEMAVVQAE
                     SGAHVVSPSGMMDGQVAAIRDGLDAAGYTDVLILAYAAKFASSFYGPFREAVESSLSG
                     DRRTYQQEPGNAREAMREITLDIDEGADIVMVKPAMAYLDVVRAAAEVSPVPVAAYQV
                     SGEYTMIRAAAANGWIDGRAAALETLTSIRRAGADIVLTYWAADVAEWLA"
     gene            27240..27665
                     /locus_tag="VF874_13565"
     CDS             27240..27665
                     /locus_tag="VF874_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177051.1"
                     /translation="MPDTSVQEQPPTRPVDVGTGFGLWLTALPLLLVGDAVDIALAPD
                     VPSHYLVYAVTGLFLVLVCAVVVTFLLLMRQGYRWARTLLTGGGLASIVYIATSLFTA
                     QRGTVAAVIYAITGIVGSVLIAGGVYLLHRKDAHGYFTR"
     gene            27688..28077
                     /locus_tag="VF874_13570"
     CDS             27688..28077
                     /locus_tag="VF874_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177052.1"
                     /translation="MTSANPRPGVLDAAYWVWVVAGILLVTFGLLAAIASAAAFFRGA
                     GVIFALAGLALGFLAGRARGGDSRSHRAAVALAVVLVLLLAVYALLSIGAIWLVIMVV
                     LIVGTFLAMRPATLAWFDTVDSGRDSG"
     gene            28070..28597
                     /locus_tag="VF874_13575"
     CDS             28070..28597
                     /locus_tag="VF874_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HEX9177053.1"
                     /translation="MADAPTPEPLFHEAGSSWSWLLAGPVAALVMLGIEINSGVGPQL
                     LVPAIFLVMVSGFLAVQVKASRIHTSVELTSETLRQGAETVPVDNIVRIFPDPDSSPD
                     ADSSPDAESQKWQSARALGELTGVPRGRTGIGLKLTQGRTAQAWARRHKELRAALTEL
                     LEQRSAENRPPGATS"
     gene            28594..28836
                     /locus_tag="VF874_13580"
     CDS             28594..28836
                     /locus_tag="VF874_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177054.1"
                     /translation="MTRAVLELVLAVAAAVGCVASWMAARSTVIVPPIADGEPTTTSV
                     TYYPPLLVLVFAMGTLAGVLTVVGVARLRRSRPAPK"
     gene            28958..30172
                     /locus_tag="VF874_13585"
     CDS             28958..30172
                     /locus_tag="VF874_13585"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9177055.1"
                     /translation="MDAHTSVTGLITGRYDDEFAEVADTFSGQIARTDGGASVAVYHR
                     GSLVVDLWGGRRNSEGDPWQRDTLAMCWSTTKGVVATCAHVLADRGELDYDERVATYW
                     PEFAQNGKADITVRQVMSHSAGLHRLGTIIGHGSEMLDWEHMTDALAQAKPAYEPGTS
                     VGYHALTFGWLVGELVQRISGTTIDEFVQKEIAEQLGLDGLYIGCPPEQRHRMAPLAP
                     MSRIIRRVTSPVGGFVFNQMTRGLQVLRSPINPHRMVNAAVSRGLEEVIVDPRLQDAA
                     VPAMNGYFDAVSLGAMYAMLAGGGQLGGVRILSEETVRKASEVQNDQRDRVVMITMQW
                     RLGYHRVPIVNKQLPLAYGHFGFGGSGAFADPEHDLALAMVCNRGSGTPIGDLRILRL
                     SQATALAVRTAG"
     gene            30225..>30748
                     /locus_tag="VF874_13590"
     CDS             30225..>30748
                     /locus_tag="VF874_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX9177056.1"
                     /translation="MTELALATPDADAQPLGPQSLVWRYFGDNRMVLIGPRPAVLQNM
                     LAELGQGVLDHSVFFDDTAARVKRSLPSIFNTVYGSDNEHPGQQVRDFHHQIKGEMPD
                     GSRYHALDPETYFWAHATFVEQLLYFADTFVKRLSRAEKEQIYLESKTWYRRYGVSAR
                     TMPADYAAFERYWDR"
BASE COUNT         4822 a        10227 c        10811 g         4888 t
ORIGIN      
        1 gagcgtgttg gtgacccgcc cgtcgccgct gggcacgaac atgcccgcga cgacgacgac
       61 gccggcgtcg gcggcgatcg atcggaccgc cgacgcccac ggtccgtgca gcggctcggc
      121 caccggcgcc agcgggacac ccaaccggca catcgtcgcc tccgggaaca ccaccagcac
      181 cgcgcccgcg tcggcggcgc gccgggtgtc gtcggcgacg agtttcaggt tggccgcggg
      241 gtctgtcccg gcgcggatct gggccagcgc gatgcgcatg cggcgagcct acggacgttc
      301 agtggtgctt ggagacccag tcggtcgtga acttctcctc ggcgtcaaac agctccgtga
      361 tatggcggat aaccagctgt tcgagccttc cgccgacgag gggaatggct accttgcaga
      421 cgctggccag ccgcagctgg cagccctggt ccgtcttggt gagcagatgc gacccccgga
      481 aatgcgcgac cacagccggg attgacgcgc cgtaggtgcc gccggcccgg ttcctgtcgc
      541 ggtcgtacgg ctcgaaatgc tgctcgcggg tgatgatcac gtccttcggc atcacggccc
      601 gcgcaatcgg cggcagatac gcgcgcggca ggttgtgcgt gaacacgact cgggtgccgg
      661 tgtggtcgct ggaaaagcgg gtgatctccg actgcggagt cagattccgg tactcctcca
      721 acagggattc ccagtagtgg cggctggtga agctgcggta gatctcctcc gccggcgcgt
      781 cgtacgtgac cgtgtagtcg agccgacgac ccatggaatc aggctagcgc cggcctcaga
      841 tgttctcgac gatccacacc gtggtgaagc gctgctcggc cacgacgagc ccggccaact
      901 tgccgccgat gaaactttcc agctttccgc cgaccaacgg aatgtcgacc tcgacggtgg
      961 cggtgaactt cagccgcgag cggccgtcct tcggcgccag caccgccgtc cccgccagcg
     1021 acaccggcgc tcccggcacg gcgccggaga cttcggcgtg cgcgacgccg tcgctgagtg
     1081 ggctccacgt ctcgttgcgc acgatctgca ggtcgcgggg gtggaactgc gtcaccatcc
     1141 cgggcagtcg gtcggtgcgc agcgtctgcg tcgtcgccac cgttacggtg ccgtcgccgc
     1201 cgacgacaat cgaattcagc gtggcgatgt cggctccgga atcggccagc cgcgccagcc
     1261 agtactcctc gtcggaaaac gccgagtgca cttggtcgac ggtgccgtgg tagttggccg
     1321 tcaagtcgaa tgaacgcggc atagcaggcc aggctaccgt taccggccgt gaccggaggg
     1381 caggagcgaa gcgacccggg aattagcagc gtcgccgaat acgccggagc gcgagtggcg
     1441 gagaacgttc cgcttgcgtc gctgaccacg ctgcgggtcg gcccggtcgc gcggcgggtg
     1501 atcacttgcg acacaacgga tcaggtcgtc gcgacactgc gtgcgctgaa ctcccagccc
     1561 ggtccggttt tggtgatggc cggcgggtcc aacgtcgtgg tcgccgacaa gatgacggat
     1621 ctgacggttg tgaagctggc caccaccggt gtcacgatcg atggcgccac gctgcgcgtc
     1681 gaggccggcg cggagtggga cgtcgtcgtc gccgagtcgc ttcggcacgg gctgggtggg
     1741 ctggagtgcc tgtccgggat ccccgggtcg gcaggcgcga cgccggtgca gaacgtcggc
     1801 gcctacggcg tcgaggtcgc cgacaccatc actggggtgc ggctgctgga ccgcggcagc
     1861 ggcgacgtgc gctgggtgcc gcccgaggag ttgggcttcg gctaccgtcg cagtgtgctg
     1921 aagaacacaa cgtcctcagt cgtgctggag gtcgaattcc ggcttgacgc gtcgggccgc
     1981 agcgcgcccc tgcgctaccg cgagctgatc accgcgttgc acgcccgcga gggagagcgc
     2041 accgacccgg tgcgcgtgcg cgatgcggtt ctcgcactgc gcagaagcaa gggcatggtg
     2101 ctcgacggcg ccgaccacga cacctggagc gtcggctcgt tcttcatgaa tcccgttgtg
     2161 gcgcaatccg attaccagag gctcgcgcag cgtagcgacg gccccgtacc gcactttccg
     2221 gcgccggacg gggtcaagct ggccgcggcg tggctcgtcg agcaagccgg cttcggcaag
     2281 ggctttcccg gtccgcaggc accggcccgg ctgtcgacca agcactcgct ggcgctgacg
     2341 aaccgcgggt cggccaccgc ggccgatgtc gtcgcgctgg cccgcacggt ccgcgacgga
     2401 gtgcgggaca aattcggtat cactttgatt ccagagccgg tgctggtcgg cgtgacgatt
     2461 taacacgtgc ggcacccttt ccgtaccctg gatcgacgtg acatcagcca acgacgcacc
     2521 gcggttcacc cgccgccgtg ccctgactgc tctcgcggtc ggggtggttg cgcctggcgt
     2581 tttggcgtcg tgtctgagca aatctggcaa acaagacgag gcggcagggc cgccaccgaa
     2641 gccgaccgtg acgttacgcc cgggaaacgg cacgaaggac gtggctccga cggccgcgat
     2701 cggcgccgag gtccgcgacg gctggttcca gcgggtcacg gtgaccaacg ccgacggcaa
     2761 aatcgtcgcc ggggcgctca gccgcgaccg cacgtcgtat tcggtgaccg agccgctggg
     2821 ctacggcatg aaatacacct gggccggctc ggtggtcggc cgcgacggga tcgcggtccc
     2881 ggtgaacggc agcttcagca ccgtcgaccc cacctcgacg gtgaacggcc agttccagct
     2941 tgccgacggg cagaccgtcg gcatcgcggt gccgatcatc ctgcagttcg acgcgtcgat
     3001 cagcgacaag gccgcggtcg aacgcgcgct cgtcgtcacc accgaacccg ccaccgaggg
     3061 cagctgggcg tggctgcccg acgaggcgga aggctcgcgg gtgcactggc ggaccaggga
     3121 gtactaccag cccggcacca ccgtgcacgt cgacgccaag ctgtacggcg tgaacttcgg
     3181 cgaggacgcc tacggcgccg aggacgtatc gctgaacttc acgatcggcc gcagacagat
     3241 cgtcaaggcg aacgcgcagt cgcaccgcat acaggtcgag accgacgggg gcgtgatcat
     3301 ggacttcgcg tgcagctacg gcgaggccga ccagccccgc aacgtcaccc gtagcggcac
     3361 ccacgtggtc agcgagaagt acgccgactt ctacatgtcc aaccccgccg ccggctacag
     3421 caacgtgcac gaacgctacg cggtgcgcat ctccaacaac ggcgagttca tccactgcaa
     3481 cccggaaagc attggcgcgc agggcaatac aaacgtcacc aacggttgta tcaatctgaa
     3541 cctggacaac gccattgagt acttcaacag cgcgatctac ggcgacccgg ttgaggtcac
     3601 cggtacgtcg attcagctgt cctactccga cggtgacatc tgggactggg cggtcgactg
     3661 ggacacctgg cgctcgatgt cggcgatcac ccccgagcag ccgccggcgt cgatcccgag
     3721 caccgcgccg gtcacgccgc cgggcgcccc caagccggtc agcggccgtc ctggcggctg
     3781 aaccgcgacg cggtggcctg gtcacgcggc cggatcacca ccaggtccag gttgacgtgt
     3841 gacgggcgtg acgccacgaa accgatgacc tcggcgacgt cgtcggcgac caacggcgtg
     3901 atgccgcgat acaccgcgtc ggcgcggtcc tggtcgccgt cgaagcgcac cagcgagaat
     3961 tcagtctcca ccgcgcccgg tgcaatctcg gtgagccgca ccggttttcc cagcagttcg
     4021 ccgcgcagcg tgcggtgcag tgcgccttgg gcgtgtttgg cggcggtgta tccggctccg
     4081 ccgtcgtagg tctccagcgc cgccaccgac gtcaccgtga cgatcaaccc gtcgccggag
     4141 tcgatgagct tgggcagcag cgcgcgggtc aggcgcagcg tgccgagcac gttggtctcc
     4201 cacatccagc gccagtgctc caggtcggcg tcggctaccg gagccaggcc cctggcgcct
     4261 ccggcattgt tgaccagcac gtccacccgg tccaggcggg cggccagcgc gtcgaccgct
     4321 gagtcgtcgg tgacatccgc cacgaccgcg gtcccgccga tctcacgagc gagcgcttcg
     4381 atccgctcgg cccggcgggc caccgcgacc acgtgaaagc cttgttcagc aagggttttg
     4441 gcggtcgcag ccccgatgcc ggagctggcg ccggtgacga cggcaacacg ggcctcagca
     4501 ctcatgcctt caacctagac cggcgtttgc tcgtcgatcg cgaaggcgtg ctaagttccc
     4561 cttcgtgttc ttcaaggctg tttcctcttc gcgcaagcgg ctcatcggcg ttgtttcctc
     4621 ttcgcgcaag cggctcatcg gcgacatctg ttgtcgcccc ggtcttcgcc gcgcctgacc
     4681 gctgagtcgg tgcggcctgg gtcccgccaa ccgactgacg aaggacacat cacgtgcgat
     4741 ccaccatctt tctcacccgc cacaacgtcg tcgcgcccgt gctgagcctg cccgaggcgg
     4801 ccgccgcggc ggtcttcggc gccgcgagga cgcgcgggcc gatcgcccgg gacgcggttg
     4861 cgcaggtcac gtcgctgtcg attgcgaccg tcaaccgcca ggtcacggcc ttgctggacg
     4921 ccgggctgct ccgggaacgg cccgacctgg cggcgtcggg agccattggg cggccccgcg
     4981 tgcccgtcga ggtcaaccac gagccgtact tgaccctggg cctgcacatc ggcgcgcgga
     5041 ccacgagcat cgtcgccacc gacctgttgg gccgcacgct cgacgtggtg gagaccccga
     5101 cgccgcagac cgggcaggtc acggcgctgc cagcgctggc ggccagcgct cggcgctacc
     5161 tgagccgctg gcaccggcgc cggccgctgt gggtcggcgt cgcgatgggc ggcgtcgtcg
     5221 acggcgccac tggaaccgtc gaccacctac ggctgggatg gatcgacgcg cccgtcgggc
     5281 cggtgctggc cgagacgctg ggcctgccgg tatcggtggc tgtgcacgtc gacgcgatgg
     5341 ccgcagcgga gctgctactg ggcatgcggc ggttcgcccc cgcaagcggg cggtacccgc
     5401 atggggccga gtcgaccagc ctgtacatct acgcccgcga gaccgtcgga tacgcgctgg
     5461 tgatcgacgg ccgggtgcac agccccgcca ccggtccggg gaacatcgcg gcgctgcccg
     5521 cccactcgga gctactgggc gggtcgggtc ggctggagtc cacggtcagc gacgaggcag
     5581 tcctggacgc cgcgcggcga ctcagcatgt ccgggagcgg gacgctggct gcggtgctgc
     5641 gggccgcgcg tgccggcagc gggcccgctc aggagctgct ggccgaacgc gccagagtgc
     5701 tgggatcggc ggtggcgctg ctgcgcgaca tgctcaatcc cgacgacctc gtggtcggtg
     5761 gccaggcgtt caccgaatac ccggagggga tgagcgccgt cgaggccgcc tatgccgaga
     5821 actcgacgct gccgccacgc gacatccggg ttgccgcgtt cggcaaccga gtgcaggagg
     5881 ccggcgccgg agtggtgtcg ctgggcgggc tgtacgccga cccgatcggc gcgatgcggc
     5941 gtgctgtccg tcgggtggga gtgtcggcct gacgccgccg gggccgcgcc cacggcagtc
     6001 tcgacgcgtg ctgcgggaat atgtcaatac cggtttcggg tagacatggc atgtgaccga
     6061 tgtttcggtg cctcggcgcg tcgcggtgct gtccgtgcat acctcgccgc tggcgcagcc
     6121 cggcaccggc gacgccgggg gaatgaacgt ctacatcctg cagtcggcgc tgaacatggc
     6181 cggccgcggc gtcgaggtgg agatcttcac ccgcgcgacg tcgtctgccg atccgccgac
     6241 cgttcgggtg gccccagggg tgctggtccg caacgtggtt gcgggccctt tcgagggcct
     6301 cgacaagcac gaccttccga cacagctgtg cgcgttcacg gccggggtgc tgcgggccga
     6361 ggccacccac gaacccggct actacgacat cgtgcactcg cactattggc tgtcgggcca
     6421 ggtcggctgg ttgacccgcg atcgctgggc ggtgccgctg gtgcacaccg cgcacaccct
     6481 ggccgcggtg aagaacgccg cgctggccga cgtcgacacg ccggagccgc cgatgcgcgc
     6541 cgtcggcgag caacaggtgg tcgacgaggc cgaccggttg atcgtcaaca ccgaagacga
     6601 agcgcgccaa cttatttcgc ttcatagcgc cgaccccgaa cgcatcgacg tcgtgcaccc
     6661 gggtgtcgac ctgcagctgt tcacgccggg cgacaagacc gccgcgcggg cggcgctggg
     6721 tctcggcgac gacgagcagg tggtggcgtt cgtcggccgg attcagccac tgaaggcgcc
     6781 tgacatcctg ttgcgggcgg ccgcgaagtt gacgggacgc tacccacggc tacgggtgct
     6841 ggtggccggc gggccctcag gctcggggct ggaagctccc gatgcgctga tgcggctggc
     6901 cgtcgagctt gggatcggca cgcgcgtgac gttcctgcct ccgcagtcac cgtcagaatt
     6961 ggtgacggtg taccgcgcgg cggatctcgt tgccgtgccg agtcattcgg agtcgttcgg
     7021 gttggtcgcg atcgaggcgc aggcgtgcgg caccccggtg gtcgcggccg aggtgggcgg
     7081 gctgccagtt gcggtgcgcg acggggtcag cggcgcgctg gtagctggcc acgatgtcga
     7141 cgactgggcc gagacgatcg gcgcgatgct ggaccgcgga ggtcccgcga tgacccgggc
     7201 cgcggtcgcg cacgccgcga acttctcctg ggcccgcacc gtcgacgcgc tgctggccgc
     7261 ctacggccgc gcgatcgccg actacagcac ttcccgtcag cgcagcccgg tggtcacgct
     7321 ggcgtcgcgg cgcaatgggc gccggtgggc gttgaggcgg gggatccggg cttgaacgtg
     7381 cagaaggtca tcgaggacgc gctcgacgag cattcgctgc cgtattttca ccatgacggc
     7441 gcccacggcg ggccgcccga catcatcgtc gagctgcccg gtgagcgccg gctcaagacc
     7501 accacgatcc tgagcatcgg cgaacactcg gtgcgcgtgg aggccttcgt ctgccgcaag
     7561 ccggacgaga accacgaaac cgtgtacggc ttcctgctca aacgcaaccg ccgcctctac
     7621 ggcgtcgcgt acacgctcga caacctcggg gacatctacc tcgtcggccg gatggcgctg
     7681 tcgtcggtga ccgccgacga gatcgaccgg gtgctcggcc aggtgctcga ggccgtcgac
     7741 caggacttca acacgctgct ggaactcggc ttctccacct cgatccggcg ggagtgggcg
     7801 tggcgggtgt cgcgcggcga atcgctgaag aacctggagg cgttcgccca cctcgtcgat
     7861 gagcgctcgc gcgaagagca tgtcatcgat aaggacgcgc cgtcagcccc gtgagagact
     7921 tccggcatgg gagatgtcgg cacgctcgtg ctgctgcgcc acggcgaaag cgagtggaac
     7981 gccaagaatc tgttcaccgg ctgggtggac gtcgacctga ccgagaaggg cagggccgag
     8041 gcgatccgcg gcggcgagct gatcgccgag cagaacctgc atcccgacgt gctgtacacg
     8101 tcgctgctgc gccgcgcgat caccaccgcg cacctggcgc tcgacgaggc cgaccggctg
     8161 tggataccgg tcgagcgcag ctggcggctc aacgagcgcc actacggcgc gctgcagggg
     8221 ctgaacaagg ccgagaccaa ggagcgctac ggcgaagagc agttcatgaa gtggcggcga
     8281 agctatgaca ccccgccccc gccgatcgag cgcggcagca cgttcagcca ggacgccgac
     8341 ccgcgctacg tcaacatcga cggcggcccg ctcaccgagt gcctggccga cgtggtggcc
     8401 cggttcctgc cctactacac cgacgtgatc gtgccggacc tgcggctcgg gaagacggtg
     8461 ctgatcgccg cgcacggcaa ctcgctgcgc gcgttggtga aatacctcga cgacatgtcc
     8521 gacgacgacg tcgtcgggct gaacatcccg accggcatcc cgctgcgtta cgacctcgac
     8581 gagaacctgc ggccggtggt gcccggcggg cagtatctgg acccggaggc ggcggccgcc
     8641 ggtgccgccg cggtggccag ccagggtgcg gacgcgagga gcaaataagc gcaggttcgg
     8701 caaacactcg gtgaacgcca gccgaatacc ttcgcgtacg cgtgtgacat gccccaaaat
     8761 ggccgcactc tgctggggtg acgttcgccg ggtgcgtacg atttggccgt gactgtggcg
     8821 tcagcggtcc tcgtggccgc ggtggccgcc ttggcggcgc tgatcttcgg cctcgccgtc
     8881 ggcgccgggg tgatgccccg aatcgccgaa cgcaaccggc ggatcgtcgc ccgggaggcc
     8941 gggctgacgg tgtcgcagat gctgcagcgc atcgtggcgc tggcacccac cggcatggcg
     9001 gtcgtcgaca cccaccgcga cgtcgtcttc atgaacgccc gcgcgcagga actcggactc
     9061 gtgcgtgacc ggctgctcga cgaccgggcg tgggcggccg cgcgccgggt gctggccagc
     9121 ggcgaggacg tcgacatcga cctgtccgcc ggccggtccc catcgggccg gtccgggttt
     9181 tcggtgcgcg gacgggtgcg gctgctcgcc gaggaggacc gccggttcgc ggtcatctac
     9241 gtcttcgacg actccgaagt ggcgcgcatg gaggcgaccc gacgcgactt cgtcgccaac
     9301 gtcagccacg agctcaagac accggtcggg gcgatggggc tgttggccga ggcgctgctg
     9361 gagtcggccg acgatcccga gacggtccgc cgcttcggcg agaagatgca cctggaatcg
     9421 cagcggctgg cgaacatggt caacgagctg atcgagctgt cccggctcca gggggcggag
     9481 cgactgcccg aactggaggc cgtcgacgtc gataccgttg tcgccgaggc gctttcgcgc
     9541 tcaaaggtgg cggccgacaa cgcgtacatc tcgatcgcca ccgacaagcc cagtggcttc
     9601 gaggtgatgg gcgaccaagc gctgctcgtc accgcgctgg ccaatttggt gtcgaacgcg
     9661 atctcgtatt cgccgccggg ctcgccggtg tcgatcagca ggcgccgccg cgggggcaac
     9721 gtcgagatcg cggtcaccga ccggggaatc ggcatcgcgc gggagcacca gcagcgagtg
     9781 ttcgagcggt tcttccgggt ggacaaagcc cgatcacgcg cgaccggcgg caccggactg
     9841 ggcctggcga tcgtcaagca catcgcggcc aaccacaacg gcaccatcag gctgtggagt
     9901 cagccgggaa ccgggtcgac gttcacgctg tcgatccccg cctacgaaga cgccggcgac
     9961 gagtcggacg gatgagagga aatacagtgt cgtgaccagt gtgctgatcg tggaggacga
    10021 ggagtccctc gccgacccgc tggcattcct gctgcgcaag gagggattcg aggcgacggt
    10081 ggtcgccgac gggcccaccg cgctggccga gttcgaccgt gggggagccg acatcgtgct
    10141 gctcgacctg atgctgcccg ggatgagcgg cacagacgtg tgcaagcagc tgcgggcgcg
    10201 ctcgagcgtg ccggtgatca tggtgaccgc gcgtgacagc gagatcgaca aggtggtcgg
    10261 gctggagctc ggcgccgacg actacgtcac caagccgtac tcggcgcgcg agctgatcgc
    10321 ccggatccgg gccgtgctgc gccgcggcac tgatgccgac gacttcggct tcgacgacgg
    10381 ggtgctcgag tcgggaccgg tgcggatgga cgtcgagcgc catgtggtgt ccgtcaacgg
    10441 cgagccgatc acgttgccgc tcaaggagtt cgaccttctg gagtacctga tgcgcaacag
    10501 cgggcgggtg ctcacgaggg gccagctgat cgaccgggtg tggggcgccg attatgtcgg
    10561 cgacaccaag acgctggacg tgcacgtcaa gcggctgcgc agcaagatcg aggccgaccc
    10621 cgccaacccc gtgcacctgg ttacggtgcg cggcctgggc tacaagctcg aagggtaggt
    10681 cggcagcgcg aacgcgaacg gccgcgggaa gccgaggccc tcgagccggc tgcgccggat
    10741 cgcgtcgaag ctgcacgtca gcgtgtcacc cgaagacggt tcgtagcaac agaattcgta
    10801 accggcgacg ccgaccagca gcacccagtg ccgcggaatc acgttgccga tcagcatggc
    10861 aaccggcgcc ccggacgcca cggcggtagc cacgtcggcg agcgtgtccc gccggccgcg
    10921 gaacagtcgc caccggtacg gccggtgcgc cgacagcgca tgcgccatcc cggccggggt
    10981 gaggccgagg cgcctgggcc atacgcggtt gaccgcggcg tgcacgcgca gctgttcttc
    11041 ggcgaaccgg cgatcatcgg ccagcaccgc ggcatagtcg gggtcgagcc gcgcaccggc
    11101 caccaccgcg acggccgggc cgcagctcac gccgtcgcgc tgacgcagga tcctcacccc
    11161 gtgatgccgc ccatgatctc ggccacgtcg aaggacgcga ccccggccac gattaccggg
    11221 tcttgctcgc cgagctcgac gacctcggcc tggctgccgg cgcgcacgac gcccagcccc
    11281 cacacgcctt cggggtcggc gactgggccg tacaccacca cccggcccgc gtctagcagc
    11341 ccccgccagt acgcggcgtg acgcgccatc gcgtcctgct cctcgggtgt catcgtctgc
    11401 gcaaaatcgg cgcgcggcgg cagcagcctg aacaggaaga gcttgtcgct catttggaag
    11461 gacgctcggc cgcccgggtg gcggggtgca ccgcgatcag tcctaatccc ttgcggcgct
    11521 tgcacattgc cgccagctcc gcgtacgcct tctcacccag cagctcggtg agctcggcgg
    11581 ccagcgactc ccacacctgc ttgcgcccga cgtgcacggc cgggtcgccg ctgcagtacc
    11641 agtgcaggtc cgcgccgccc tccccccagc cgcggcggtc gaactcggtg atcgtggtct
    11701 tgagaatctc ggagccgtcg ggccgcgtca cccactcctg gctgcgccgg atcggcagct
    11761 gccagcacac ctccggcttc atcgtgagcg gctcgacccc gagccgcatc gccttgatgt
    11821 gcaacgcgca cccgatgccg ccggagaagc cgggacggtt gaggaaaatg cacgcgccct
    11881 tgtacttgcg agtgcgccaa gacttctcgc cgtcgtactc gtcgcgctcc agataaccct
    11941 tgcggccgag gcccttctcc cggaactgcc agtctttggc ggtcagcttc ttgacggcgt
    12001 cgtccagctg cgcgcggtcg tcgtcgtcgg tcaaaaacgc tccgtgcgag caacatccgt
    12061 cgtcgggccg gccctccacc gtgccgcggc aggcgggcgt gccgaacaca cacgtccagt
    12121 tcgacaacaa ccacgtcatg tccgcggcga tcagatgctc ggggttgtcg gggtcgtaga
    12181 actccatcca ctcgcgggcg aagtccaatt ccacctcacc ggggtgcgga gcgctcacag
    12241 aggacaacgg tagtcgcatg agtgcacacc tggcgactag gcgcgctcta cgtgttcagg
    12301 tggacgatcg acaatggcgg caacgtcccg ctgagcgtga cgcaagtgca agcagggcag
    12361 caggatccga tgatggccgc ggtacttccc gatgggcttg accgagtaga gcatccctcg
    12421 ccggggtgaa atcaaccaca cccgagggca gtccgattcc ccccgctgag cgatggatcg
    12481 tcatgctcgt cgacacgaac cttgagtgga cgcgcaacag acgcgaaaca caaccaacca
    12541 accgggagta catcatgagc acgatcatca accgaaagtc agtcaccgca gcgctcggtg
    12601 caacggtcgc cgcggcgacg gtaccggcgc tgctggtcct cggcgcagga accgcgcagg
    12661 caggtacgtc cgcctggacc accaccgatg cgttgggggt cacggtccgc gcccgaggct
    12721 cacgcctgcc tcatggtttt caagtgtaag cagaagccct gtcgcagaga atgattcatg
    12781 tgtggtaggt caggatctgc gcaccgaccc gcaggatcga tccgggctcg agttccgttg
    12841 gcgtcgtgcc gatccgggtc cagttctcgg cgccggggcc cacgatgagg ccaaagggcg
    12901 tccggacggg gtctgcgccg aaggtgagga gcgcccgact aaaccgtcac ccggcgcgat
    12961 gcccacggtg tatggatttc ggtccacccc gcgcaccctt cgtgaattcg ccccgtgatt
    13021 ctagtccgtt cgacggcgca ggtggcgcta cccactaggt tgaagggcgt gcgattgggt
    13081 gtgctcgacg tcggcagcaa caccgtccac ctgttggtcg tcgatgcccg gcgcggtggt
    13141 catcccaccc cgatgagttc gacgaaggcg tcgctgcggc tggccgaggc gatcgacgac
    13201 tccggcaagc tcacccgcaa aggcgcggac aagctgatct ccaccgtcga cgagttcgcc
    13261 aagatcgccg ccagctccgg ctgcgccgag ctgatggcgt tcgccacctc ggcggtgcgc
    13321 gacgcgaaga actccgaaga cgtgctggcc cgcgtgctcg ccgagaccgg agtggcgctg
    13381 caggtgctca gcggcgtgga cgaggcgcgg ctgacgttcc tggcggtgcg ccggtggtac
    13441 ggctggagcg ccgggcggat catcaacatc gacatcggcg gtggatcact ggagttgtcc
    13501 agcggcgtcg acgaggagcc cgacgtcgcc atgtcgctgc ccctgggcgc gggccggttg
    13561 acccgcgagt ggctgcccga cgatccgccc ggccggcgcc gggtcgccat gctgcgcgac
    13621 tggctggata ctgagctggc tgacgctagc cgtaccctgc tcaaggcagg tccgcccgat
    13681 ctggcggtag cgtcgtcgaa gaccgtgcgg tcgctggccc ggctcaccgg cgcggcgcct
    13741 tcggccgcgg gaccgcgggt gaaaagaaca ctgacagcca ccggactgcg gcagttgatc
    13801 gcgttcatct cgcggatgac tgtggccgac cgggctgaac tggaaggagt gagcgccgag
    13861 cgatcgccac agatcgtggc gggagcgttg gtagcagagg cgagcatgaa agctctgtca
    13921 atagaaaccc tcgacatttg cccgtgggcg ttgcgagagg gcctgattct gcggaaactc
    13981 gacagcgaag ctgatggaac ggcgcttgtg gagacatccg tgggggatgc tggtcacgtg
    14041 ggcgccaaac gacgagcggg caactctcga tgaccggacc acacgataac tccgacaccc
    14101 ggccgatttc ggtcgcggag ttgctggccc gcagcggggt catcggggcg ccgcccgtcg
    14161 gcggccgtcg gcgcaagcgc aggggcaacg cggacgccgt caccgtcgcc gaactcaccg
    14221 gcgagatccc gatcatccgg gacgaggagc ccgccgtcga gcaggcgccg gcagaaacga
    14281 acggcaccgt ggacacggtc gccgagcccg agccggaggt ccagcccgag gtagaccagg
    14341 aggtcgagcc tgtcgacgtg ccggcggtcg ccgaagatct cgctgacgcg gatgtccccg
    14401 cagaggagat gcttcccgat cacgtcgaca tcgacttcga cgaggacacc gacgaccacg
    14461 acgcagcggc cgcggtggat gaggatctcg acgagaccga cgagaccggc gagccgccgt
    14521 cctatctcga tgctgtccag gagccgttgt tcggcggccg caccgacgac gacctggtcc
    14581 gcgacgacga gaccgcccag tccgtcaccg agctgcccga cgtgccgccg gagaccacca
    14641 ccgccgagga ccacgaggag gtggcaccgc ccacccgttt gcagcagatc ggccgcggcg
    14701 cgctggtggt gctgcagtcg attctcgcgg tcgcgttcgg cgccgggctt ttcattgcgt
    14761 tcgacgaact ctggaagtgg aacaacatcg tcgcgctggt gttgtcagtc ctggtgatcc
    14821 tcggcctggt ggtcggggtt cgcatcgtcc gcaagaccga agatatcgcg agcacgctga
    14881 tcgcagtcgc ggtcggtgct ctggtcacac tgggcccgct ggcgctgctg caatcgggct
    14941 aggcggctcc caaacactgt gcgcccagcg atcaaagtcg gtctgtcgac tgcgtcggtt
    15001 tacccgttga ggaccgaggc ggccttcgag tacgccgccc gcctcggcta cgacggcgtg
    15061 gagctgatgg tctgggccga gtcggtcagc caggacgtcg acgaaatcaa caaactgtcc
    15121 cagcactacg ggatgccggt gctggccgtg cacgcgccgt gtctgctgat ctcgcagcgc
    15181 gtgtggggcg ccaacccgat cccgaagctc aaccgcaccg tgcgtgccgc cgagaagctg
    15241 ggcgcgcaga ccgtcgtcat ccacccgcca tttcgctggc agcgccgcta cgcggacggc
    15301 ttcaccgagc aggtcgcccg gctggaggcg tccagcgacg tgatcgtcgc cgtcgagaac
    15361 atgtttccgt tccgcgccga ccggttcttc ggtgggacgc aggcgtcgat cgagcggatg
    15421 cgcaagcgcg gcggccggcc agggcccgcg atttcggcgt tctcgccgtc gtatgacccg
    15481 ctggaaggca accacgccca ctacacgctc gacctgtcgc acaccgcgac cgcgggcacc
    15541 gacgcgctgg acatggcccg ccgaatgggc tcgggcctga ctcacctgca cctggccgac
    15601 gggtccggcg cctacctcga cgagcacctg gtgccggggc gcggcacgca gccgacgttc
    15661 gaggtgtgcc agatgctggc ggcgagcgat ttcgccggcc atgtgatcct tgaggtcacc
    15721 acgtccgggg cgcgcaccga ggcggagcga gaagtcctgc tgaccgagtc gctggaattc
    15781 gcccgcaccc acctgctgcg ctgaaggaag ctgatgactg aggccagcct gttttcgaac
    15841 gcgatggcgt tgcgcccgct gggtgacggg gtctttgcgg gcgagttgaa caagatctgg
    15901 accatcgggc cgaagctgca cggcggcgcg atgctgtcgc tgtgtgcggt cgcggcccgg
    15961 gaagccgtcg gcggcgtcgt ccagccgatc tcggtgtcgg ccaactacct gtccgccccg
    16021 agccccgggg acctgcggct ggagacgttc gtccgcaaaa ggggtcggcg gatcagcgtc
    16081 gtggacgtcg agctgatcgg cggggaccga gtcggcgatg agcgctcgcg cgaagagcag
    16141 aaaacagccg tgcacgccgt ggtgacgctg ggggagcccg agcacctggc tccacccttg
    16201 ctgtcggcga atcccgtggt gtcgctgatg agccccgaac caccgcccga cgtcgagccg
    16261 atcgggccgg ggcacccgat gttcggcacc gttaactact gggagggcat cgacgtgcgg
    16321 ccgctgatgt ccaccgttcc cgacgggccg tccgcagacg gccgaacccc ggtgatccag
    16381 atctgggcgc ggcctcgcga catggcgccc gacatcctgt tcgcgctgat gtgcggcgac
    16441 gtctcgatgc ccgtcgcgtt cgcggtgggc cgccgcggct gggcgccgac cgtgcagctc
    16501 accgcctatc tgcgaggctt gccggccgac ggctggctgc gggtgatggc cacgacgacg
    16561 cagatcgggc aggactggtt cgacgaagat catcttgtcg tcgacagcgt gggccggatc
    16621 atcgtgcaga cccgccagct ggcgctggtg cccgatcact gaggcgtctg gcatgctgct
    16681 gccgtgtcca gaatcgcgat catcgggggc gggaacatcg gcgaagcgct gctgtcgggg
    16741 ctgctgcgct cgggccggca ggtcaaggac atcgtggtcg ccgaacgcgt tccggcgcgc
    16801 acgcgacacc tgtccgagac gtactcggtg ctggtgacca gcgtggccga cgcggtggag
    16861 aacgcgaact tcgtcgtcgt cgcggtcaaa ccttccgacg tggagtcagt gaccgacgag
    16921 atcgccgcag cggtcgcgcg cgacgacgcc ggcagcatcg agcacgtgtt cgtgaccgtc
    16981 gtcgcgggcg tcagcaccac ctacttcgag ggcaaactgc cggccggtgc gccggtcatc
    17041 cgggtgatgc ccaatgctcc gatggtggtc ggcgccggag tcagcgcgat agccaagggc
    17101 cggttcgcga ccccggagca gctgaaggac gtcgccgcgc tgttcgattc ggtgggcggg
    17161 gtgctcaagg tgccggaatc gcagctcgat gcggtcacgg cggtgtccgg ctcggggccg
    17221 gcctacttct tcctgatggt cgaggcgctc atcgacgcgg gcgtcgcggt ggggttgagc
    17281 cgcgcagatg ccaccgacct cgtcgtacaa acgatggccg ggtcggccgc gatgctgctg
    17341 gagcgcgtcg acgaaaacgg tggatccggt gatatcgaca ccacgcccgc ccggttgcga
    17401 gccgccgtga cgtcgcccgc cggcaccacc gcggccgggt tggccgagct cgagcgcggc
    17461 ggattgcggg ccgctgtcgc cgccgccgtg gtggccgcaa aaaaccgcgc tggccagcta
    17521 ggagttacat cagagtagtt catgaatttt cgacggattg ccccacaccc gtcgcagtaa
    17581 tcccacctgt accgctattc tcctcgatgt atgcacgtgt tcgtgccagc ggaggggaag
    17641 ccgctggcct tggccgtgcc cggaggattg ggttgcgatg acgtcaacga acgggccatc
    17701 ggggcgggat tcggcaagtg gtaagcggga ttctggcgct gccgacgttc agccggccgc
    17761 cagggcccaa tttctcaccg tcgccgaggt ggccagcttg atgcgggtca gcaagatgac
    17821 ggtctaccgg ttggttcaca acggcgaatt gcccgccgtt cgggtgggcc ggtcatttcg
    17881 ggtgcacgcc aaggccgtgc acgacctgct ggaaacgtcg tacttcgacg tcagctgaat
    17941 ccgtctgctc ttcgcgcgag cgctcatctg aatccgtctg ctcttcgcgc gagcgctcat
    18001 cgcccgcgat tctccttctt gcccgtcagc cgggtaaggt gtccgcgtcc ctgggtagac
    18061 aggacttaga cagacacggg caggactagg cagcggagtt catgggttca gtcatcaaga
    18121 aacggcgcaa gcgcatgtcg aagaagaagc accgcaagct gcttcgtcgt actcgggtgc
    18181 agcgcagaaa actcggcaag taacccggct cgcgcgggcg tcgtccatcg acatgcccga
    18241 ctcggtaccg atcgttagga tgtcgggatg gattccgcag gttccgacac cttccagtcc
    18301 cgcaaagtgg tgttggtcac gggcgcctgc cgatttctcg gcggctatct gacggcccgg
    18361 ctggttcaga atccggtgat cgagcgggtg atcgctgttg acgcgatcgc accgagcaag
    18421 gatttgctgc gcagaatggg gcgtgcggag ttcgtccgcg ccgacattcg aaacccgttc
    18481 atcgccaagg tgattcgcaa caacgacgtg gacaccgtgg tgcatgcggc ggctgcgtcg
    18541 tatgcaccac gcgcgggctg gcgggccgcg ctcaaggaac tcaacgtgat gggcgcgatg
    18601 cagctgctcg ccgcctgcca gaaggcgccc gccgtgcggc gggtggtgct caagtcgacg
    18661 tcggaggtgt acggctccag cacgcgcgac ccggtggtgt tcaccgagga ctgcaccagc
    18721 cgccggccgc tcggcagcgg gttcgcccaa gacagcctcg acatcgaggg ctacgcgcgc
    18781 ggcctgggtc gccgccgccc ggacattgcg gtgacgatcc tgcgcctggc gaacctgatc
    18841 ggtccggcca tggacacggc gctgtcgcgg tatctcgccg gtccgttcgt gccgaccgtg
    18901 ctcggccgtg acgcccgcct gcagttgctg cacgagcagg acgcgctggg cgcgctggaa
    18961 cgcggcacga tggcgggtag ggccggtacg ttcaacgtcg gggccgacgg gatcatcatg
    19021 atgtcgcagg ccatccgcag ggcgggccgg atcgcgctgc cggtgcccgg attcggtgtc
    19081 tgggccgtcg attcgcttcg gcggctcaac aactacacag agatcaatcg cgagcagctg
    19141 aactacctga gtttcggcag ggtgatggat accacgcgaa tgaaggtcga actggactac
    19201 catccgaagt ggtctacggc tgaggcgttc gacgattacg ttcgcggtcg tggtctgaca
    19261 ccaataattg acccacgatg ggtacgctcg ctggaacgcc gtgcggtggc aaccgcgcaa
    19321 cgttgggggt aaatgcagac acactcgagc aagcgggtgg gaagaggtat cagtagtgtc
    19381 gggtgaaccc aaggccaaag tgattccgct gcatggggat tcgggcccgg cgcagcgacg
    19441 cgccgcccgg cgtgccgagg ccgccagccg acatccctcg ctgctgtcca atccggacag
    19501 cagcggctca gcggaggaca tcgccgccgt cgtccgtgag atcgacgcgc atcgaagtac
    19561 cgccgcgagc ggcgcgccgg agcaacccga agagcccaac gagctggctc gccggatcgg
    19621 cgcggtcgcg gagttcatgc gtcgccgatt caccggtgac tacgacgtcg acgagttcgg
    19681 gttcgacccg catttcaacg acgcggtcgt gatgcctgtg ctgcgaacgc tgttcgcgtc
    19741 gtggttccgg gtcgacgtga ccggcgtcga gaacctgccg gaaactggtg cggcgctggt
    19801 cgtggccaac cacgcgggtg tgctgccgtt cgacgggctg atggcctcgg tcgcggtgca
    19861 cgacaagcac ccggcgcagc ggactttgcg gttgctggcc gccgacatgg tgctcgacag
    19921 cccgctgctg ggtcaggccg cgcgcaaggc cggccacacc atggcgtgca cggccgacgc
    19981 gcaccgcctg ttgtcgaaag gtgagctcac cgcggtattc ccagagggct acaaaggcct
    20041 gggcaagcgg ttcaaggacc gctacaagct gcagcggttc ggccgcggcg ggttcgtgtc
    20101 ggccgcgctg cgcaccaggg cgccgatcat cccgtgctcg atcgtcgggt cggaggagat
    20161 ctacccgatg atcggcgacg tgcggctgct ggcgaggctg ttcgggttgc cctacttccc
    20221 gatcacgccg ctgttcccgc tggccggccc gatcgggatg atcccgctgc cgtcgaagtg
    20281 gcacatcgag ttcggtgagc cgatcccgac cgccgactac gacgacgcgg cgccggagga
    20341 cccgatgatc acgttcgagc tcaccgacca agtgcgcgag accattcagc agacgctgta
    20401 ccggctactc gccaaccggc ggaacacctt cttcggctga gtcggcccgg gccttgccca
    20461 gcgctttggc gatcgccgca tcgcggcctg cggcgatcgc cgacaccacc tcgtcggcct
    20521 cgtcgtcgta ctgtgcttcg ccgatcactg tgacgacgct ggtgatcacc ggctggccgt
    20581 cggcgtcggt gacctcgccc cggatctcgg tgatgaccgt gccgtgcgac tcgatgaccg
    20641 agtccaggta ggagtcgaag aacagcttgt cgccggccag gatcggccgg tgaaatttca
    20701 gcttctgatc ccggtgcaac acccgctcca tgttgatcgg cacatcgaac tggtcgaaga
    20761 tctccagctg cactttgcgg cccgccacgg tgaggaacgt cagcgacgcc acgagcgccg
    20821 gatagccgag ttcggcggcc gcgtcttcgg aaaagtgcgc agggtgatcg tcttgcaccg
    20881 cacgggcgaa ctcgcggatc ttctcccggt ccaccgcgaa atagtcggga tagcgatagt
    20941 gcgtaccgat gatttcttcg gcaatggcca tgtcggcgca gcctatcagc ggctgtttcc
    21001 tcttcgcgca agcgctcatc agcggcggcg cgacgcgatc gcagccagcg cgccacccgc
    21061 ggcgcccagc gccagcgccg acggcacacc gatgcgcgcg gccttgcgcg cggtccggaa
    21121 gtcgcgaatc tcccatccgc gatgccgggc gagttcccgc agcgcgctgt ccgggttgat
    21181 cgcgacggcc gttccgacca gcgagagcat cggcacgtcg ttgtagctgt cggagtacgc
    21241 ggtgcagcgt ttcaggttga ggccctcgcg gatggccaga gaccgcacgg cgtgcgcctt
    21301 gccgctgccg tgcaggatgt cgccgaccag caggccggtg aacacgccat cgacggattc
    21361 cgcgaccgtg cccagcgcgc cggtcagccc gagcttcttg gcgatcgtgc tggccagctc
    21421 gcacggcgtc gcggtgacca gccacacctg ctgcccggcg tcgaggtgca tctgggccag
    21481 cgccctggtg cccggccaga tcttgtcggc gatgatgtcg tcgtaaatct cttcccccac
    21541 tgccaccagc tcggccgtcg accggccctc gatgaacgcc agcgccttgc gccggccggc
    21601 ggccacgtcg tcgctgttct cgcgtccggt gagctgaaac ttggcctgcg cgtagacgaa
    21661 tcggatcaga tcgctgtagt tgaagtactt tcgcgcggcg agtccgcggg cgaaaagcac
    21721 cagcgacgac ccgtgcacca gcgtgttgtc gacatcgaag aacgcggccg ccgtgaggtc
    21781 gacgggcggt ggcggcgcgg ccccagggcg tggcggctgc ggcagctcgg cattccgcgc
    21841 gacatcggcc gcgaggtgag cgacggcggc ttccgcgctc gcatcgccgg cgatgccctg
    21901 cgagtcggga tccgtcaccg ttcaacagta ggacacccaa tggggatact tgcgacgtga
    21961 accggcgggt gacgttgctg acgcgcgacg gctgcgcgac gtgtgaacag gctcggctgc
    22021 gactctgcga gttggccggt gagctgggct ttgacctgtc ggtgaccgac gtcgacgccg
    22081 aggcggcgga tggccggcca ggatcgcgcg ccgagttcgg ggaccggctg ccggtggtgc
    22141 tgctcgacgg gcgtgagcac agctactggg aggtcgacga gccgcggctg cgggccgatc
    22201 tgctgcgaaa ctgacgtttc gcacggagag tgcgagtggg aacgtgcgaa acgggggttt
    22261 cgcggaaaat ggcggcaatc gagccagaat ttggtcggct aaccaccaat tgattaccgt
    22321 ggataccgtg gtgattcagc cgtgagcgtg ctgcttttcg gggtgtcgca ccgcagcgca
    22381 ccggtgcagg tgctcgagca gttgagcagc ggcgagtccg atcaggtcaa gatcgccgac
    22441 cggctgctgc agtcgtcgtt gatcaccgag gcgatggtgc tgtccacctg caaccgcgtc
    22501 gaggtgtacg cggtcgtgga cgccttccac ggcggcctgt cggtgattgg ccaagtgctg
    22561 gccgagcatt cgggcatgtc gctgaacgat ctgaccaagt acgcctacgt gcgctacgcc
    22621 gaggcagccg tcgagcatct gttcgccgtg gccagcgggc tggattcggc ggtggtcggc
    22681 gagcagcagg tgctcggcca ggtccggcgg gcgtatgcgg ccgcggaggc caatcgcacg
    22741 gtgggcaggg tcatgcacga gctagcccag cgtgcgctgt cggtgggcaa gcgggtgcac
    22801 accgagacca gcatcgacgc cgccggcgcc tccgtcgtgt cggtcgccct cgggatggcc
    22861 gagacgcgcc tggacacctt ggccggccgc accgccgtcg tcgtcggcgc gggcgcgatg
    22921 ggcgcgctgt cggccaagca gctcgcccac gcgggcgtcg agcgcgtgca cgtcgtcaac
    22981 aggtcgctgc cccgcgccag aaggctcgcc cacaacatcc gcgaggtcgg cgtcagggct
    23041 gacgcctcag cgcttgacca cctgcccaat gtgctcgccg aggccgacgt cgtcgtgtgc
    23101 tgtaccggcg cagtccggcc ggtggtcagc ctcgccgacg tgcaccgcgg gctcgcgcac
    23161 ggcgccgaac cccgccggct ggtgatctgc gacctgggca tgccgcgtga cgtcgatgcc
    23221 gcggtcgcgg gtttgccggg cgtgttcgtc gtcgacatgg agcgcattca gcgagagccg
    23281 tcggcgcgcg cggccgccgc cgacgccgag gccgcccgca atatcgtggc tgccgaagtg
    23341 gcgcaatacc tttcggctca gcggatggcc gaggtcaccc cgacggtgac ggcgttgcgg
    23401 cagcgcgccg ccgacgtggt cgagacggaa ctcctgcggc tggacaaccg gctgcccgac
    23461 ctggccggcg ctcaccgcga ggaagtcgcc cgcaccgtgc ggcgggtggt cgacaaactg
    23521 ctgcacgcgc cgacggtccg cgtcaagcag ctggccagcg cccctggcgg cgacagctac
    23581 gccgaagcgc tgcgcgaact tttcgaactc gaccagcagg ccgtcgacgc cgtcgcggcc
    23641 ggcgagctgc cgctggcgat cgacccagaa tccgggtaac gcttgaccat caggatcggc
    23701 acccggggca gcttgttggc caccacccag gccggtgtca tcagagacgc gttgatcgcg
    23761 aagggccacg ccgcggagct ggtgaccatc agcaccgagg gcgaccggtc ggccgagccg
    23821 atcgccacga tcggggtcgg cgtgttcacc gccgcgctgc gggaggcaat cgacgacggg
    23881 atcgtcgata tggccgtgca ctcctacaaa gatctcccga ctgccgagga caagcggttc
    23941 accatcgccg cggtgcccag gcgtggagat ccgcgagacg cgctggtggc acgcgacgga
    24001 ttggtgctcg gggagctgcc gacgggttcg gtgatcggca cgtcgagccc gcgacgggcc
    24061 gcacagctta gagcattggg tctcggtttg gaaatccgcc ccctaagagg caaccttgac
    24121 accaggttga acagggttat gagcggtgat ttggacggca tcgtggtggc ccgggcgggt
    24181 ctggcccgca tcggacgcct cgacgatgtc accgagacgc tggagccggt gcagatgttg
    24241 ccggcgccgg ctcaaggcgc gctcgcggtc gagtgccgca gcgccgacac caagctcgcc
    24301 gcgctgttgg ctgagctcga cgacgccgac acacgcgcag cggtcaccgc agaacgagtc
    24361 ctgctcgccg aactggaggc ggggtgctcc gcaccggttg gcgcgatcgc cgaggtggtc
    24421 gagtcgatcg atgaggacgg ccgcgtcttc gaagagctgt cgctgcgcgg ctgcgtggcg
    24481 accgtggacg gatccgacgt gatccgtgcg tccgggatcg gaacacccgg gaaggctgcg
    24541 gagcttggtc gctcggtggc cgccgagctg ttcgacctgg gcgcccggga gttgatggga
    24601 acgaggtagg aatgacgcga gggcgcaagg ccaggccagg gcgcgtcgtg ttcgtgggtt
    24661 cggggcccgg tgaccccggg ctgctgaccg ggcgggcgcg cagcgtgctg tcctgcgcgg
    24721 agttggtgtt caccgatccc gacgtgccgg ctgaggtgct ggccgtggtc gggtcgagcc
    24781 tgcccgatcc gccgaagctg tcgtcaccgg acccgggcat caccgacgac accgtggagc
    24841 cgaacccgcc gctggtgccg gacatccgcc ccgcgctggg cgatcccgcg gaggtggcca
    24901 agattctggc cgcggaggcc cgcgccggtg ccgacgtcgt ccggctggtc gccggtgacc
    24961 cgctgtcggt ggacgcggtg atcaccgagg tgcaggcgat tgccaaggcg cacttgctgt
    25021 tcgagatcgt ccccggtctg ccggccagca ccgcgatacc gacctatgcg gggctgccgc
    25081 tgggttcgtc gcacaccgtc gccgacgtgc gcggcgacgt ggactgggcg gcgctggccg
    25141 cggcgccggg cccgctgatt ctgcacgcca ccgccgagca tctggccgac gcggcccgca
    25201 cgctgatcga ctacggcatc gacgacggga cgccgtgtgt ggtgaccgcg aacggaacca
    25261 ccacgcagca gcgctcggtg gagggcacgc tgcagacgct gacggacagc gcggcgctcg
    25321 gcgggtcgga tccggccggg ccgtcccccg ccggcgcgtg ggccctgacc ggtccgttga
    25381 tcgtgacgat cgggcggacc gtcgccagcc gggcgaagct gaactggtgg gagagccgcg
    25441 ccctctacgg ctggacggtg ctggtgccgc gcaccaagga tcaggccggc gacatgagtg
    25501 agcggttgat cacccacggc gccgtgccga tcgaggtgcc gaccatcgcc gtggagccgc
    25561 cgcgcagccc ggcccagatg gagcgcgccg tcaagggatt ggtcgacggg cgctaccagt
    25621 gggtggtgtt cacgtccacc aacgcggtgc gcgcggtgtg ggagaaattc ggcgagttcg
    25681 gcctggacgc gcgcgcgttt tccggcgtga agatcgcctg cgtcggccaa gccaccgccg
    25741 accgggtacg cgcgttcggg ataaccccgg aactggtgcc gtcgggtgag cagagctcgc
    25801 tggggctact cgacgaattc ccgccctacg acgacgtttt cgacccggtc aaccgggtcc
    25861 tgctgcctcg cgccgacatc gccaccgaga cgctggccga aggactgcgc gaacgcggtt
    25921 gggagatcga ggatgtgacg gcctaccgca cggtgcgggc cgcgccgcct cccgccggga
    25981 cccgcgagat gatcaagtcc ggcgggttcg acgcggtctg cttcacgtcc agttcgacgg
    26041 tgcgcaacct cgtcggcatc gccggcaagc cgcacgcccg gaccatcgtc gcgtgcatcg
    26101 gaccgaaaac cgccgagacg gcagccgaat tcgggctgcg ggtggatgtg cagccggagg
    26161 tggcgcagat cggcccgctg gtcgacgcgc tggctgagca cgccgcacgg ctgcgtgccg
    26221 agggcgctct gccgccgccg cgtaagaaga gccgcaggcg gtagctgtgg ggttcccccg
    26281 ccagcgaccg cggcggctgc gctcgacgcc ggcgctgcgt cggctcgtgg cccaaacatc
    26341 tttggagcca aggcatttgg tgctgccgat gttcgtcgct gacggcatcg acgagccgcg
    26401 tccgatcgcg tcgatgcctg gtgtggtgca gcacacccac gacagtctgc gtaaagcggc
    26461 cgcggacgcg gtggccgcgg gcgtgggcgg aatcatgctg ttcggcgttc cccgcgagga
    26521 ggacaaggat ccgaccggtg aggccggcgt caccatcgac ggtgtgctga atcgggcgtt
    26581 gcaggaattg aacaaggatc tgggctcggc cacggtgctg atggccgaca cctgcctcga
    26641 cgagttcacc gaccacggtc actgcgggtt ggtcgacttc tcgggtcgag tcgacaacga
    26701 cctcaccctg tctcgatatg tggaaatggc tgtggtgcag gctgagtcag gtgcccacgt
    26761 ggtctcaccg agcggcatga tggacgggca ggtcgccgcg attcgcgacg gtctcgacgc
    26821 ggccgggtac accgacgtcc tcatcctcgc ctacgcggcg aagttcgcgt cgtcgttcta
    26881 cggcccgttc cgggaggcgg tggagtccag cctgtccggt gaccgccgca cgtaccagca
    26941 ggagcccggc aacgcccgag aagcgatgcg cgagatcacg ctggacatcg acgaaggcgc
    27001 cgacatcgtc atggtcaaac ccgcgatggc gtacctcgac gtggtgcgtg cggcggcgga
    27061 ggtgtcgccg gtaccggtgg ccgcgtacca ggtgtcgggc gagtacacga tgatccgcgc
    27121 cgcggcggcc aacggctgga tcgacggccg agcggccgcc ctggagacac tcaccagcat
    27181 ccggcgggcg ggcgccgaca tcgtgttgac gtactgggct gctgatgtcg cggagtggct
    27241 tgcctgacac gtcggtgcag gagcagccgc ccacccggcc ggtggacgtc ggtaccggct
    27301 ttgggctgtg gctgacggca ctgccgctgc tgctggttgg cgatgccgtc gacatcgcgc
    27361 tggcgccgga cgtgccgtcg cattacctcg tgtacgcggt caccgggctg ttcttggtgc
    27421 tggtgtgtgc ggtagtggtg acgttcctgc tgctgatgcg gcagggctac cgctgggcac
    27481 gcacgctgtt gacgggcggc gggctggcct cgatcgtcta catcgccacc agtttgttca
    27541 cggcccagcg cggcacggtc gccgcggtca tctacgcgat caccgggatc gtcggctctg
    27601 tgctgatcgc cggtggcgtg tatctgttgc accgcaagga cgcccatggg tatttcacgc
    27661 gctaggcacc gataggctgg tccgatcatg acgtcagcta acccgcggcc gggggtcctc
    27721 gatgccgcct attgggtgtg ggtggttgcg gggatcctgc tggtgacatt cgggctgttg
    27781 gcagcgatcg cctctgctgc ggcgtttttc cggggggctg gggtgatctt cgcgctggcc
    27841 ggtctggctt tgggtttcct cgccggccgg gcgcgcggcg gtgatagccg gtctcatcgt
    27901 gccgcggtgg cgttagccgt ggttctggta ttgctgctgg cggtgtacgc gctgttgagc
    27961 atcggagcga tctggttggt gatcatggtc gtgttgatag tcggtacctt cctcgcgatg
    28021 cggccggcaa cgctggcctg gttcgacacg gtggattccg ggagagacag tggctgacgc
    28081 gcccacgccc gagccgctgt tccatgaggc cgggtcaagc tggtcgtggt tgctggccgg
    28141 acccgtcgcg gcgctggtca tgttgggcat cgagatcaac tcgggcgtgg gaccgcaatt
    28201 gctggtgccg gccattttcc tggtcatggt gtcgggcttt ctggcggtgc aggtcaaggc
    28261 gtcgcgcatt cacacatcgg tcgagctgac ctcggagacc ctgcgccagg gcgccgagac
    28321 cgtccccgtc gacaacatcg tgcggatctt tcccgacccc gactcgtcac ccgacgccga
    28381 ctcgtcaccc gacgccgagt cgcagaagtg gcagtcggcc cgcgcgctgg gggagctgac
    28441 cggcgtgccg cgcggacgca ccggcatcgg gctcaaactc acccaaggcc gcacggcgca
    28501 ggcctgggcg cgcaggcaca aggaactgcg tgccgcgctg accgagctgc tcgagcagcg
    28561 cagcgccgag aaccggccgc ccggcgccac gtcgtgacgc gcgccgtcct cgagctggtg
    28621 ttggccgtcg ccgccgcggt ggggtgcgtg gcgagttgga tggcggcgcg gtccacggtg
    28681 atcgtcccgc ccatcgccga cggcgagccc acgaccacct cggtgacgta ctacccgccg
    28741 ctgctcgttt tggtgttcgc gatgggcacc ctcgccggcg tgctgaccgt cgtcggtgtg
    28801 gctcggctca gacgctcccg tcccgcgccg aaatagcgtt ccaggtcgtg gtttgggcag
    28861 aagcacagcc ctggcttcac tctcggcggc cggcgagcga cgttacattt tcccgagaat
    28921 gcgtcgcgga acgcggggat gacggacact tccatcagtg gacgcacaca catcggtcac
    28981 gggcttgatc acgggccgct acgacgacga gttcgccgaa gtggccgaca ccttcagcgg
    29041 ccagatcgcc cgcaccgacg gcggcgcctc ggtcgccgtc taccaccgcg gcagtctcgt
    29101 ggtcgacctg tggggcgggc gccgcaacag cgagggcgac ccctggcagc gcgacacgct
    29161 ggccatgtgc tggagcacga ccaagggcgt cgtcgccacc tgcgcccatg tgctcgccga
    29221 ccgcggggag ctcgactacg acgaacgggt cgccacgtac tggccggagt tcgcccagaa
    29281 cggcaaggcc gacatcaccg tccggcaggt gatgagccac tccgcgggcc tgcaccggct
    29341 cggcacgatc atcggccacg gatcggagat gctggactgg gaacacatga ccgacgcatt
    29401 ggctcaggca aagccggcct atgagcccgg aacctcggtg gggtatcacg cgctgacgtt
    29461 cggttggctg gtcggtgagc tggtgcagcg gatttcgggc acgacgatcg acgagttcgt
    29521 ccagaaggag atcgccgaac agctcgggct cgacggcctg tacatcggct gcccgcccga
    29581 gcagcggcac cggatggcgc cactggcgcc gatgtcgcgc atcatcaggc gtgtcaccag
    29641 ccccgtcggc ggcttcgtgt tcaaccagat gacccgcggc ctgcaagtcc tcaggtcgcc
    29701 gatcaacccg caccggatgg tcaatgcagc ggtgtcgcgc ggtttggagg aggtcatcgt
    29761 ggaccctcgg ctgcaagatg ccgccgttcc cgcgatgaat gggtacttcg acgcagtgtc
    29821 gctgggcgcg atgtacgcga tgctcgcagg cggtggccag ctaggcggtg tgcgcatcct
    29881 gtctgaggag acggtacgca aggcctccga ggtccagaat gaccaacgtg accgggtcgt
    29941 catgatcacc atgcagtggc ggcttggcta tcacagggtc ccgatcgtca acaagcaact
    30001 ccctcttgcc tatggccatt tcggtttcgg cggctccggc gccttcgccg atcccgagca
    30061 cgacctggcc ctggcgatgg tgtgcaaccg gggcagcggc acaccgatcg gggacctgcg
    30121 cattctgcgc ctcagtcagg cgacggccct cgccgtccgg acggcggggt gaaccccgtc
    30181 acaaggattt tggtacagag tgcgagattt gtaacactgt ttccatgacc gagcttgcgc
    30241 tggcgacgcc agacgcagac gcccagccgt tggggcccca gtctctagtc tggcggtatt
    30301 tcggcgacaa ccggatggtc ctgatcggcc cgcggccggc ggtgctgcag aacatgctcg
    30361 ccgaactggg ccagggcgtg ctggaccact ccgtgttctt cgacgacacc gccgcccgcg
    30421 tgaagcgctc gctgccgtcg atcttcaaca ccgtctacgg ctccgacaac gagcacccgg
    30481 ggcagcaggt ccgcgacttt caccaccaga tcaaggggga gatgcccgac ggcagccgtt
    30541 accacgcgct cgatccagag acgtacttct gggcgcacgc gacgttcgtc gagcagctgc
    30601 tgtatttcgc cgacaccttc gtcaagcggc tcagccgcgc cgagaaggaa cagatctacc
    30661 tcgagtccaa gacgtggtat cgccgctacg gcgtcagtgc gcggacgatg cccgccgact
    30721 atgccgcgtt cgagcggtac tgggatcg
//
LOCUS       DASWBF010000363         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1221595,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000363 DASWBF010000000
VERSION     DASWBF010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221595"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VF874_13595"
     CDS             <1..1226
                     /locus_tag="VF874_13595"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9177057.1"
                     /translation="RRRTLDAGVSKTDVIAGRYDDDFTEVARVFRDQITHTDGGASVA
                     VYHRGRLVVDLWGGVRIADGDSWQRDTLAMCWSTTKGVTATCAHVLADRGQLDYDERV
                     ATYWPEFAQNGKADITVRQVLSHSAGLHRFGTIIDHGSKILDWEHMTDALARARPAYE
                     PGTSVGYHALTFGWLIGELVRRISGVPIEKFVQTEIAEPLGLDGLYIGCPPEQRHRMA
                     PLAPMSKAFKRLSDPGGSFLFNQVTRGLHLLRSPINPHRMINAALSRGMEEVIADPRL
                     LDAAVPALNGYFDAVSLSAMYAMLAGGGQLGGVRILSQDAVRQASAVQNDQRDRVVMM
                     TMQWRLGYHRALFVKQLPRAYGHFGFGGSGGLADPEHDLALAMVCNRGSGTPMGDRRI
                     LRLSQATARAVKSIN"
     gene            complement(1256..2485)
                     /locus_tag="VF874_13600"
     CDS             complement(1256..2485)
                     /locus_tag="VF874_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEX9177058.1"
                     /translation="MTARSRRAGDIQPEPLYILGAGMHPWGKWGNDFTEYGVVAARAA
                     LAEAGLDWRQIQFVAGADTIRNGYPGFVAGATFAQKLGWNGVPVSSSYAACASGSQAL
                     QSARANILAGFCDVALVVGADRAPKGFFAPVGGERRDDPDWQRFHLIGATNPVYFALL
                     ARRRMELYGATIEDFAQVKVKNAKHGLHNPNALFRKESSVDDVLASPVVADPLRLLDI
                     CATSDGAAALVVASAEFARKHLGSLDGVPSVRAVSTVTPRYPQHLPELPDIATDSTAA
                     VPAPERVFKDQIIDAAYAEAGIGPEDVRVAEVYDLSTALELDWYEHLGLCARGEAEAL
                     LRSGATTIGGRVPVNPSGGLACFGEAIPAQAVAQVCELTWQLRGQATGRQVEGATVGI
                     TANQGLFGHGSSVIVAR"
     gene            complement(2482..2925)
                     /locus_tag="VF874_13605"
     CDS             complement(2482..2925)
                     /locus_tag="VF874_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX9177059.1"
                     /translation="MPEAIDEAKPAIDGWFATDESGSPHLIGSRCPACGTYVFPPRAL
                     NCPNPACESGTLESVALARRGTLWSYAENRYAPPPPYPASDPFEPFAVAAVQLADEGL
                     IVLGKVEKGTLADDLKVGMEMELTIEPLYRDDDGVDRTVYAWRIA"
     gene            3346..3642
                     /locus_tag="VF874_13610"
     CDS             3346..3642
                     /locus_tag="VF874_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177060.1"
                     /translation="MDDTTIEELHQSAAGHDDVVAVSWLALAGYDHPSPVSADDDLGV
                     DAAAVVLADGGDRLVAHWDQGAVDEPLRIGWWQTARPARGWSDGLANGSAPAAP"
     gene            3800..4342
                     /locus_tag="VF874_13615"
     CDS             3800..4342
                     /locus_tag="VF874_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177061.1"
                     /translation="MIAMATACTDTTAVSSGDVVPRRRGRSKAANFKYDGPVSVIGLE
                     LDVSDPMVRRRLERQWAAAFRLRRGCGLCGDRDIVSAILAACVTLADPDDPRTARVDY
                     RLAHALRAWLASQQEWEGSVNRHQPPPSSDAGSATTGSHPRVASAEQAALGLPPNSPG
                     TPGRRGPSRKQPVSKLIGAA"
BASE COUNT          699 a         1397 c         1505 g          758 t
ORIGIN      
        1 gcagaaggcg cacattggac gctggggtat cgaaaaccga cgtaatcgcc ggtcgctacg
       61 acgacgattt caccgaagtg gcgagggttt ttcgcgatca gatcacccac accgacggcg
      121 gtgcctcggt cgccgtctac catcgcggcc ggctggtcgt ggatctttgg ggcggcgttc
      181 gcatagccga cggggactcg tggcagcgtg acaccctcgc aatgtgctgg agcacgacca
      241 agggtgtcac cgcgacctgc gcccatgtgc tcgccgaccg cggccagctg gactacgacg
      301 aacgcgtggc tacgtattgg cccgagttcg cccagaacgg caaggcggac atcacggttc
      361 ggcaggtgtt gagccactcc gccgggctcc accgcttcgg cacgatcatc gaccacggat
      421 ccaagatcct cgactgggag cacatgacag atgcgcttgc gcgggccagg ccggcatacg
      481 agccggggac gtcggtcggc tatcacgcgc tgacgttcgg ctggctcatc ggcgaactgg
      541 tgcggcgaat ctcgggcgtg ccgatcgaga agttcgtgca gaccgagatc gccgaaccgc
      601 tcgggcttga cgggctgtat atcggctgcc cgcccgagca gcggcaccgc atggcacccc
      661 tggccccgat gtcgaaggcc ttcaagcggc tgtcggaccc tggcggcagc ttcctgttca
      721 accaggtgac gcgcggtctg cacctgctga ggtcgccgat caacccgcat cggatgatca
      781 acgcagctct ctcgcgcggt atggaggaag tcatcgccga cccacgactg ctcgacgccg
      841 ccgttcccgc gttgaacggg tatttcgatg cggtgtcgct gagcgcgatg tatgcgatgc
      901 tcgccggcgg agggcagctc ggcggcgtgc gcatcctgtc ccaggacgcg gtgcgccagg
      961 cgtcggcggt ccagaacgac caacgcgacc gggtcgtcat gatgaccatg caatggcggc
     1021 tcggctacca ccgggcgctg ttcgtcaagc agctgccgcg tgcttacggc cacttcgggt
     1081 tcggcgggtc cggcggcttg gccgatcccg aacacgacct cgccctagcg atggtgtgca
     1141 accgcggcag cggcacgccc atgggcgatc ggcggattct ccgcctcagc caagccaccg
     1201 ccagagctgt caaatcgatc aactaggtcc tcgcccgggg atcgtccgaa ccaattcagc
     1261 gggcgacgat caccgacgat ccgtgtccga agaggccttg gttggcggtg atgccgacgg
     1321 tggcgccctc gacttggcgt ccggtggctt ggccgcgcag ttgccaggtc agttcgcaga
     1381 cttgggcgac ggcttgagca gggatggctt cgccgaagca ggcgagcccg ccggacgggt
     1441 tgacgggcac gcgaccgccg attgtcgttg cgccgctgcg cagtagcgcc tcggcttcgc
     1501 ccctggcgca caacccgaga tgttcgtacc agtcaagttc cagtgcggtc gacaggtcgt
     1561 agacttcggc gacgcggacg tcctctggcc cgatgccggc ttcggcgtag gcggcgtcga
     1621 tgatctggtc cttgaacacc cgctcgggtg ctggcactgc cgcggtggaa tccgttgcga
     1681 tatctggcag ttcgggtaga tgctgcggat agcgtggcgt gaccgtgctg accgcgcgca
     1741 ccgatggcac gccgtcgagc gagccgagat gcttacgagc gaactccgca ctggccacca
     1801 ccaacgcggc cgcgccgtca gacgtcgcgc aaatgtccaa cagccgcaac ggatctgcca
     1861 ccaccgggct ggccagcaca tcatcgaccg acgactcctt gcggaacagc gcattgggat
     1921 tgtgcaaccc gtgcttggcg ttcttgactt ttacctgcgc gaagtcctcg atcgtcgcgc
     1981 cgtacagttc cattcgccgg cgcgccagca acgcgaagta caccgggttc gtcgcgccga
     2041 tcagatggaa ccgctgccag tccggatcgt cgcggcgttc gccgcccacc ggtgcgaaga
     2101 agcccttcgg cgctctgtcg gctcccacca ccaacgcgac gtcgcagaat cctgccaaga
     2161 tgtttgcgcg cgcgctctgc agtgcctggg aaccgctggc gcacgccgcg taactcgagc
     2221 tgaccggcac gccgttccag cccagttttt gcgcgaaggt ggcgccggcg acgaacccgg
     2281 ggtagccgtt gcggatcgtg tcggcgccgg cgacgaactg gatctgccgc caatcaagtc
     2341 ctgcctccgc cagcgccgca cgggccgcga ccacgccgta ctcagtgaag tcgttgcccc
     2401 acttgcccca cgggtgcatc ccggcgccga ggatgtacag aggttcgggc tggatgtcgc
     2461 cggccctgcg gctccgggcc gtcatgcgat cctccacgcg tagacggtcc gatccacgcc
     2521 gtcgtcgtcg cggtacagcg gctcgatcgt caactccatc tccatcccga ccttgagatc
     2581 gtcggccagc gtgcccttct cgaccttgcc gagcacgatc agcccctcgt cggccagctg
     2641 caccgcggcg accgcgaacg gctcgaacgg gtccgacgcg ggatacggag gcggcggcgc
     2701 gtagcggttc tcggcgtagc tccacagcgt gccgcgtcgc gccaacgcga ccgactcgag
     2761 cgtgccgctt tcgcaggcag ggttggggca gttcagtgct cgcggcggga agacgtacgt
     2821 cccacaggcc gggcagcggc tgccaattag atgcggcgat cccgattcgt cggtggcgaa
     2881 ccagccgtcg atcgccggtt tcgcctcgtc aatagcttct ggcacgtcgt caggttacct
     2941 acgatcgccg gaaaactgaa acgtgttcca gttcggctcg gtggcgcgcc gagactgcgg
     3001 attcgcagcg gaagttcgag tggaggcctg ccggaacaca gtttcggcgc aagcggtgac
     3061 ggctgacctg atgtcggcgt tggtgcctag gaggtgaccc gtgattgctt tcgggtttga
     3121 gggttagagt ccggcgatgg tgtggaggct gtgattgatg gcgtcgccgc ggtggcggca
     3181 ttgccgcggt gtttgccgac cggtccttga gccgggtgat gacgtgcttg gcgcgggctt
     3241 tgatccggcg gtgcacccgg tagtggtcgt cgcggatgat ccgcggccgc agtggtcgcg
     3301 tcgcggggtg gcgatcgagg tgatggcgcg gtatccgccg tcgccatgga cgacacgacc
     3361 atcgaggagc tgcaccagag tgcggcgggc cacgatgacg tcgttgcggt tagctggctg
     3421 gcactggccg gctacgatca cccgtcgccg gtgagcgcag atgatgatct gggcgttgac
     3481 gctgcggcgg tagttcttgc tgatggcggt gatcgactgg tcgcgcactg ggatcagggt
     3541 gccgtcgatg aaccactgcg gattggctgg tggcaaacag cgcggccagc gcgcggatgg
     3601 tcagatgggt tggcaaatgg atcagcacca gcagcaccct gactggcagc ggcagacccc
     3661 agggccttgc agggggtggc gatgtgcggt gtcgggtctg gggcagacga tgtgcaccgg
     3721 tgtggcggtg agcccggtga gcggcaccaa gtttcggtat cggtgtgggg gcgggtcgat
     3781 gtcgggcggt ggtggtttca tgatcgctat ggccaccgcg tgcaccgaca ccaccgccgt
     3841 tagcagcggt gatgtggtgc cacggcggcg ggggaggtcg aaggcggcga atttcaagta
     3901 cgacgggccg gtgtcggtga tcggtctgga acttgacgtc agcgacccaa tggtgcgccg
     3961 gcgcctggag cggcagtggg cggcggcgtt tcggctgcgg cggggctgcg ggctgtgcgg
     4021 tgaccgtgac atcgtctcgg ccatcttggc ggcctgcgtg acgctcgccg accccgacga
     4081 tccgcgcact gcgcgggtcg attaccgact tgcccacgcc ctgcgggcgt ggctggcctc
     4141 ccagcaagag tgggagggtt cagtcaaccg gcaccagcca ccgccatcat cagatgctgg
     4201 atcggccaca accggcagcc accctcgggt ggcctctgct gaacaagcag cactcggcct
     4261 acccccgaac agcccaggca cacctggacg tcgcggaccc agccgaaaac aaccagtgtc
     4321 caagctgatt ggcgctgcat gacccactac gagtcaact
//
LOCUS       DASWBF010000364         4713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_766188,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000364 DASWBF010000000
VERSION     DASWBF010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766188"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="VF874_13620"
     CDS             complement(<1..1558)
                     /locus_tag="VF874_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9177062.1"
                     /translation="MLDTDTTVGQALFRVSWDRYGGSVGSTARTIGRVRAFVRWVIRT
                     PWPVFTLGMLQADIIGALFVLGYLRFGMPPQDRVVIQELPQANLALFGSVLFLAFTFG
                     SLVSLRLLMPVFRWQRRDRDGLLDADPVSTELARTRALKMPFYRSVISAINWFLGGTI
                     FIIAIWPVASHSAPVVAVATALGATATSIIGYLQAERVLRPVAVAALREGVPEQIHAP
                     GVILRTLLTWVLSTGVPILAIVVAIGAAKLSILHASADQLFMPILLLAIAALIIGLAG
                     TILTAMSIADPLRQLRWALGEVQRGNYNAHVQIYDASELGLLQAGFNDMVRELSERQR
                     LRDLFGRYVGEDVARRALERSPELGGQERDVAVLFVDLVGSTQLAATQPPAVVVGLLN
                     EFFRIVVDTVDRHGGFVNKFQGDAALAIFGAPLEHPDASGAALAAARELHGELIEMLG
                     ANEFGIGVSAGRAIAGHIGAQARFEYTVIGDPVNEAARLTELAKLEEGHVLASAIAVS
                     AALDAEALCWD"
     gene            1573..2781
                     /locus_tag="VF874_13625"
     CDS             1573..2781
                     /locus_tag="VF874_13625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX9177063.1"
                     /translation="MSGVFGRLVGQGAVEAELIAAARAARGDSAHNASVTGTMTHAWL
                     ITGPPGSGRSVAAVCFAAALQCTSDGEPGCGRCKACVTTMAGTHADVRRIVPEGLSIG
                     VDEMRGIVQIASRRPSTGRWQVVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPS
                     VDPEDIAITLRSRCRHVALVTPTVDAIAQVLVDTDGLSTEDAQWAASVSGGHVGRARR
                     LATDPAARERRERALGLVRDAVKPSRAYAAAEELVATAETEARALTAGRDETEAEELM
                     TALGAGGTGKGTAAAMRGATGAIKELERRQKSRLTRSSRDALDRALIDLANYFRDALL
                     VASGAGEVRANHPDMAERVQALADHASPDRLLACIEAVLECREALATNVKPKFAVDGL
                     VAAVGQALRE"
     gene            2864..2939
                     /locus_tag="VF874_13630"
     tRNA            2864..2939
                     /locus_tag="VF874_13630"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2897..2899,aa:Thr,seq:cgt)
     gene            complement(3023..>4713)
                     /locus_tag="VF874_13635"
     CDS             complement(3023..>4713)
                     /locus_tag="VF874_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177064.1"
                     /translation="YFYWDDLILTGRAGTQNILSPSYLFDDHDGHVMPGAFLVAGVIT
                     RLAPLNWTGPAISLVVLQLLASATLLRALWVILGWRPVLLIPLTFALFTPLGVPGYAW
                     WAAALNSLPMLAAMAWVCADAILLVRTGDRRYAVTGLLAYFGGLLFFEKAAVIPFVAF
                     AITALLAYVTDGESALRTTWQRGKHLWTSSLALTAVWVVLYLVVVDQERWSSDLAMTW
                     NLLRRSVTHGIVPGLAGGPWSWDRWAPASPWATPSPVVMALGWVTLATVLAVSLIRKQ
                     RIVPVWLLAAGYAITCQVPIYLMRSSRFTALELAQTLRYFPDLVVVLALCAAVAFCAP
                     NRRTSGWLDRSAVRTAVTACLTATFVVSSLYSTATFLTSWRDNPAQPYLQNAKRTLAQ
                     ARSSSEAPLLDQEVDPLILQRVAWPENLASHMFALLTDRPEFSSATSKLRIIDATGHV
                     VDAKVTWVRTVTTGPAPQCGYFVQTDQPVRMPLDGPLLPADWTTEINYLANTEGSMNM
                     SLSDGQETKVPVHPGLNRVFVRLPGAGDAINVRANTAALTLCIASGPVGFVAPV"
BASE COUNT          819 a         1451 c         1724 g          719 t
ORIGIN      
        1 cgtcccagca caacgcctcg gcgtccagcg cggcactgac cgcgatcgcc gacgccagca
       61 cgtggccttc ctcgagcttg gccaactcgg tcaaccgtgc cgcctcgttg accgggtcac
      121 cgatgaccgt gtactcgaac ctggcttgcg cgccgatgtg gccggcgatc gcgcggccgg
      181 cggagacgcc gataccgaac tcgttggcgc cgagcatctc gatcagctcg ccgtgcagtt
      241 cacgggcggc tgccagcgca gcgcccgacg cgtccgggtg ttccagcggt gcgccgaaga
      301 tcgccagcgc ggcgtcgccc tggaacttgt tgacgaaccc gccgtgccgg tcgaccgtgt
      361 cgacgacgat ccggaagaac tcgttgagca gcccgacaac cacggcgggc ggctgcgtgg
      421 cggccagttg ggtggacccg acgaggtcga cgaacaacac cgcgacgtcg cgctcctgcc
      481 cgcccagctc gggagaacgc tccaacgctc gacgtgcgac gtcctcgccg acgtagcggc
      541 cgaacaggtc gcgcagccgt tgccgttcgg acagctcgcg gaccatgtcg ttaaacccgg
      601 cctgcaacag gccgagctcg ctggcgtcgt aaatctgcac gtgcgcgttg tagttgcccc
      661 gctgcacctc gccgagtgcc caccgcagtt ggcgcagcgg atcggcgatc gacatcgcag
      721 tcaggatggt gccggccagg ccgatgatca gcgccgcgat cgccaataac agaatcggca
      781 tgaacagctg atccgccgaa gcgtgcagga tcgacaactt ggcggcgccg atcgccacca
      841 cgatcgccag aatcggcact ccggtggaca gcacccatgt cagaagggtg cgcagaatga
      901 cgccgggcgc atggatttgc tccggcacgc cttcgcgcag ggccgcgacg gcgaccggcc
      961 gcagcacccg ctcggcctgc aggtagccga tgatcgacgt tgctgtggcg cccagcgcgg
     1021 tggcgacggc gaccaccggc gccgagtggc tggcgaccgg ccagatcgcg atgatgaaga
     1081 tcgtcccgcc gaggaaccag ttgatcgcgc tgatgacgga ccggtagaac ggcatcttca
     1141 acgcacgcgt gcgcgccaac tccgtcgaga ctgggtcggc gtcgagcagt ccgtcgcgat
     1201 cgcgccgctg ccaacggaac accggcatca gcaggcgcag gctgacgagt gacccaaatg
     1261 tgaaggccaa gaacagaact gagccgaaca acgccaaatt cgcttgcggc agctcctgaa
     1321 tcaccacccg gtcctgaggt ggcatgccga agcgtagata tccgagcacg aacaacgcgc
     1381 cgatgatgtc ggcctgcagc atgccaagcg tgaataccgg ccagggggta cggatcaccc
     1441 accggacgaa tgccctgacc cgcccgatcg tcctggccgt acttcccacg gatccaccgt
     1501 atcggtccca gctgacgcgg aatagcgctt gtccgacggt ggtgtcggtg tcgagcacta
     1561 gtgttgtcgg tgatgagcgg ggtttttggc cgtctggtgg gtcagggggc ggtggaggcc
     1621 gagttgatcg cggccgcgcg cgcggcacgc ggtgattccg ctcacaacgc gtccgtgacg
     1681 gggaccatga cacacgcctg gttgatcacc ggcccgcccg gctcggggcg gtcggtcgcg
     1741 gccgtgtgct ttgctgcggc gctgcagtgc acctccgacg gcgagccggg ttgcgggcgc
     1801 tgcaaggcgt gtgtgaccac gatggcgggc acgcacgccg acgtccgccg gattgttccg
     1861 gaggggctct cgatcggtgt cgacgagatg cgcgggatcg tgcagatcgc ctcgcggcgg
     1921 cccagcaccg ggcggtggca ggtggtggtg atcgaagacg cagaccggct cactgagggc
     1981 gcggccaacg cgctgctcaa ggttgtcgaa gagccgccgc cgtcgacagt gttcctgttg
     2041 tgcgcgccgt cggtggatcc ggaggacatc gcgatcacac tgcggtcgcg ctgccggcac
     2101 gtcgcgctgg tgacgccgac ggtcgacgcg atcgcgcagg tgttggtcga caccgacggg
     2161 ctgtcgaccg aggacgcgca gtgggccgcg tccgtcagcg gtgggcatgt gggccgggcg
     2221 cggcggctgg ccaccgatcc ggcggcgcgg gagcgtcgtg agcgagcgct gggtttggtg
     2281 cgcgacgccg tgaagccgtc gcgggcttac gccgcggccg aggagctggt ggccaccgcc
     2341 gagacggaag cgcgggcgct gaccgccggt cgggacgaga ccgaggccga ggagctgatg
     2401 acggcactgg gcgccggcgg aaccgggaag ggcacggcgg cggcgatgcg cggcgccacg
     2461 ggcgcgatca aagagctgga gcggcggcag aagtcgcggc tgacgcggtc gtcgcgtgac
     2521 gcgctggatc gcgcgctcat cgatttggcg aactacttcc gcgacgcgct gctcgtcgcc
     2581 tcgggggcag gcgaggtccg ggcgaatcac cccgacatgg ctgagcgtgt gcaggcgttg
     2641 gccgatcacg cgtccccgga ccggctgctg gcgtgcatcg aggcggtgct ggagtgtcgg
     2701 gaggcgttgg ccaccaacgt caagccgaag ttcgcggtcg acggactggt cgcggcggtg
     2761 ggacaggcgc tgcgcgagtg acttgggtgg gccgtgcagg ctgccataga ctcgtgccgc
     2821 ccggcatgca tgtggctctt cgcgcaagcg ctcatccggg cacgccacct tagctcagtc
     2881 ggcagagcgg ctcactcgta atgagcaggt caggagttcg attctcctag gtggctccat
     2941 cttttgcggc tcaccttttt cgcgagcgtg cgtgtttgta cccctacgct ggtccagggc
     3001 ggcattttgc gcacgctcgg tgctagacgg gcgcgacgaa accgacggga ccggaggcga
     3061 tgcacagcgt cagcgcggcc gtgttggcac gtacgttgat cgcgtcgccc gcgccgggca
     3121 gacggacgaa cacgcggttc agtcccggat gtaccgggac tttcgtctcc tgcccgtcag
     3181 acagcgacat gttcatcgat ccttcggtgt tggccaggta gttgatttcg gtggtccagt
     3241 cggcgggcag tagcgggccg tcgagcggca tcctgacggg ttggtcggtt tggacgaagt
     3301 agccgcattg cggcgcgggc ccggttgtga cggtgcgcac ccaggtgact ttggcgtcga
     3361 cgacgtggcc ggtcgcgtcg attatcctga gcttgcttgt ggcagaggag aactcaggac
     3421 gatcggtgag caatgcgaac atgtggctgg ccaagttctc aggccaggcg acgcgctgga
     3481 ggattagagg atcgacctcc tggtcgagca gcggcgcctc cgacgacgac cgagcttgcg
     3541 caagagtgcg tttcgcgttc tgcaggtacg gctgggccgg attgtcacgc cagctggtca
     3601 ggaacgtggc ggtggagtag aggctggaaa cgacgaaagt tgctgtgagg caggcagtca
     3661 cggctgtgcg aaccgctgac ctgtcgagcc agccagatgt gcggcggtta ggggcgcaga
     3721 aggcgacggc ggcgcagagg gccaacacga cgacgaggtc ggggaagtag cgcagtgtct
     3781 gggcgagttc gagcgcggtg aacctcgacg accgcatgag gtagatcgga acttgacagg
     3841 tgatggcgta gccggcggcg agcagccaca cgggcacgat tcgttgcttg cgaataaggg
     3901 agaccgccaa caccgtggcg agtgtgaccc agccgagggc catgacaacg ggcgacggag
     3961 tggcccaagg ggatgccggt gcccagcgat cccacgacca gggaccgccg gcgaggccgg
     4021 gcacgatgcc atgagtaacg gagcgcctca acaggttcca cgtcatggcg aggtcggagc
     4081 tccagcgctc ctggtcgacc actacgaggt aaagaacaac ccagacggcg gtcagcgcca
     4141 aactgcttgt ccacaaatgc tttccacgct gccacgtcgt tcttagtgcc gactcgccat
     4201 cggtgacgta tgcaagcaag gccgtgatcg cgaatgcgac aaatgggatg acggcggcct
     4261 tctcgaagaa cagaaggccg ccgaagtagg cgagcagacc ggtgacggcg tagcgtcggt
     4321 cgccggtgcg gacgagcagg atggcgtccg cacacaccca ggccatcgcg gcgagcatcg
     4381 gaagcgagtt gagggcggcg gcccaccacg cgtagccggg cacgccgagc ggggtgaaga
     4441 gcgcgaaggt cagcgggatg aggagtaccg ggcgccagcc gaggatgacc cacagcgcgc
     4501 gcagcagggt tgcagacgcg agcagttgga ggaccacgag gctgatcgcc gggccggtcc
     4561 agttcagggg ggcgagtcgg gtgatgacac cggcgacgag gaatgcgccg ggcatgacgt
     4621 ggccgtcgtg gtcgtcgaac aggtaggacg gcgacaggat gttttgggtg ccggcgcggc
     4681 cggtcaggat gagatcgtcc cagtagaagt agc
//
LOCUS       DASWBF010000365        10691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_100827,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000365 DASWBF010000000
VERSION     DASWBF010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100827"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="VF874_13640"
     CDS             complement(<1..114)
                     /locus_tag="VF874_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9177065.1"
                     /translation="MRITVLGCSGSVTGPDSPASGYLLTAPGAPPLVIDFGN"
     gene            complement(191..1012)
                     /gene="murI"
                     /locus_tag="VF874_13645"
     CDS             complement(191..1012)
                     /gene="murI"
                     /locus_tag="VF874_13645"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX9177066.1"
                     /translation="MTSALAPVGIFDSGVGGLTVARAIIDQLPDEDVIYVGDTANGPY
                     GPLAIPEVRAHALAIGDDLVAHGVKALVIACNTASSACLRDARERYDVPVVEVILPAV
                     RRAVVTTRNGRIGVIGTEATIASGAYQDAFAAARDTVITGVACPRFVDFVERGVTSGR
                     QVLGLAEGYLEPLQRAGIDTLVLGCTHYPLLSGLIQLAMGEAVTLVSSAEETAKDLVR
                     VLTELDLLRPHDAPPATRIFEATGDPEAFTKLAARFLGPALDGVRPVRRHVGASS"
     gene            complement(1009..1680)
                     /locus_tag="VF874_13650"
     CDS             complement(1009..1680)
                     /locus_tag="VF874_13650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9177067.1"
                     /translation="MGMIGPDYSTKPAPKKRPAWLTGGITILTFVALLYVIEVFNQMS
                     GDRLEDNGIRPLQSDGLWGILFSPMLHANWQHLMANTLPALVLGFLMTLAGLSRFVFA
                     TAIIWIVGGFGTWLIGDVGNTCGGNTNIVGASGLIFGWLTFLLVFGWFTRNVWEIVVG
                     LVVLFFYGTILLGALPVVGCTGISWQGHLCGGIAGVFAAYVLSGPERKQRELRRGKTP
                     PYLTS"
     gene            complement(1671..2633)
                     /locus_tag="VF874_13655"
     CDS             complement(1671..2633)
                     /locus_tag="VF874_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HEX9177068.1"
                     /translation="MTRYDSLLEALGNTPLVGLQRLSPKWDDPDHVRLWAKLEDRNPT
                     GSIKDRPALRMIEEAERDGLLKPGATILEPTSGNTGISLAMAALLKGYTLICVMPENT
                     SIERRQLLELYGAQIIYSPAEGGSNTAVAHAKELAAKNPSWVMLYQYGNQANTAAHYE
                     GTGPELLADLPEITHFVAGLGTTGTLMGTGRFLREHVPDIQIVAAEPRYGEGVYALRN
                     IDEGFIPELYDPDVLTTRYSVGSADAVRRTRELISAEGIFAGISTGAVLHAALGMANK
                     ALKAGQRADIAFVVADAGWKYLSTGAYAGSLDEAEDALEGQLWA"
     gene            complement(2665..2940)
                     /locus_tag="VF874_13660"
     CDS             complement(2665..2940)
                     /locus_tag="VF874_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9177069.1"
                     /translation="MTVSVSIPTILRSHTGGEKRVNAAGDTLQAVISDLEANYSGISE
                     RLIDNGKLHRFVNIYINDEDVRFSGGLDTEINDGDTVTILPAVAGGR"
     gene            complement(2937..3377)
                     /locus_tag="VF874_13665"
     CDS             complement(2937..3377)
                     /locus_tag="VF874_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009474136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX9177070.1"
                     /translation="MLIIRADLVDAIVAHARADHPDEACGVLAGPEGSDRPMRHIAMV
                     NAERSPTFYRFDSGEQLKVWRQMDEADEVPVVIYHSHTATEAYPSRTDVSLAAEPDAH
                     YVLVSTRDPCRHELRSFRIVDAVVTEEPVSVVDNYESAPEGAHK"
     gene            complement(3430..4470)
                     /locus_tag="VF874_13670"
     CDS             complement(3430..4470)
                     /locus_tag="VF874_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX9177071.1"
                     /translation="MISTTGSITDVGGVTVGHHHRLDHSGWATGSTVVLTPAGTVAAV
                     EVRGGAPGTRETDLLDPANTVRYVDAILLTGGSAYGLAAADGVMQWLEEHRRGIVMDD
                     GVGERVVPIVPSAVIYDLAVGDWNYRPTAEFGYAAAQGAGRDVGVGSVGAGTGARAGV
                     LKGGVGTASSVLRSGVTVGALVVVNCVGDVVDPATGLPWTNYLTSELGLAPPPPAQVA
                     AFAALAAERDRYAQAFNSTIAVVASDAMLSPPSCRRVAIMAADGLARAIRPSHSPLDG
                     DTVFVVATGAIETPPSGKLPAAMSEQAEVTGAVGAAAADCLARAVVLAVTSASAVAGI
                     PTYRDVLPGAFA"
     gene            complement(4478..5059)
                     /locus_tag="VF874_13675"
     CDS             complement(4478..5059)
                     /locus_tag="VF874_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HEX9177072.1"
                     /translation="MRKWKRVDTADGQRFRSALAAHEASMLHSLVKSMLDMLEERESS
                     TPADELEKITGIKAGNPRRPQDATMRRLLPDFYRTERNHPAGSAAAESLNAALRSLHE
                     PEIIEAKREAGQTMLRTVPVGGGRFELTREDANAWVAAVNDIRLALGTMLGIGPSGPE
                     RMPDDHPMAGHFDVYQWLTVMQEYLVLALMGQR"
     gene            complement(5081..5383)
                     /gene="clpS"
                     /locus_tag="VF874_13680"
     CDS             complement(5081..5383)
                     /gene="clpS"
                     /locus_tag="VF874_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX9177073.1"
                     /translation="MVTPARTRPGTREEVDVDVVEATDVPWVTLVWDDPVNLMTYVTY
                     VFQKLFGYSEPHATKLMLQVHNEGKAVVSAGSREAMETDVSKLHAAGLWATLQQDR"
     gene            5457..6791
                     /locus_tag="VF874_13685"
     CDS             5457..6791
                     /locus_tag="VF874_13685"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX9177074.1"
                     /translation="MTTPSEPTALLTDKYELTMLSAALRDGTAHRHTTFELFARRLPE
                     GRRYGVVAGTGRFLEALASFEFDDVALDSVRGFLDPDTVDYLARYRFRGDVDGYAEGE
                     LYFPGSPVLTVRGTFAESVVLETLVLSILNHDTAIASAAARMVSAADGRPLIEMGSRR
                     AHEHAAVAAARAAYIAGFAGSSNLAAQRRYGVPALGTSAHAFTLLHTTADGPDEQAAF
                     RAQVDALGVNTTLLVDTYDITAGVANAVAVAGTALGGVRIDSGDLGVLARQVRRQLDD
                     LGARHTRIVVSGDLDEFAIAALRAEPVDSYGVGTSVVTGSGAPTANMVYKLVEVDGMP
                     VEKRSSRKESHGGFKHAMRLARPTGTILEEVVYPAGRPPAVAEAGRELSVPLVRGGDP
                     LVDDNLTAVRARVAAGLMSLPWDGLKLSRGEPAIPTRMVPATIEEGPRQRPR"
     gene            6788..8764
                     /locus_tag="VF874_13690"
     CDS             6788..8764
                     /locus_tag="VF874_13690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX9177075.1"
                     /translation="MTDLLATAVDALGGSERAGQVTMAEAVAHAFDTGEHLAVQAGTG
                     TGKSLAYLVPAIARAVETDCSVVVSTATIALQRQLVERDLPRLADSLEHLLPRRPSFA
                     ILKGRGNYLCLNKIHNGSASDASDDGDDALFEPTAITALGRDVQRLTSWTSTTDTGDR
                     DELRPGVRDRSWAQVSVSARECLGVARCPFGAECFAEKARGRAGCADVVVTNHALLAI
                     NAISDAPVLPEHDLLVIDEAHELVDRVTAVATQELSSTPLAVAERRIARLVESELAER
                     FDAAATTFSSAIRDAAPGRIDHLDDGLATHLAALRDAATAARRAIPTTSTDPKAGAAR
                     SEAIAALDDIADTCTRILESFQAAVADRSDVVWLVNDDSRGLAQLRVAPLSVAALLSG
                     GLFNHATAVLTSATLTPGGSFDAIASDWGLTGADADFRWRGIDVGSPFSHAKSGILYV
                     ARHLPPPGRDGTGSAEQLAEIEALVSAAHGRTLGLFSSMRAAAAAAEEMRERLTTPVL
                     CQGDDATAALVERFAAEPETSLFGTLSLWQGVDVPGPSLSLVIIDRIPFPRPDDPLLT
                     ARQRAVNARGGNGFMAVAATHAALLLAQGAGRLLRRVDDRGVVAVLDSRMATARYGRF
                     LRDSLPPFWFTTDRHTVLAALDRLTAGPDGGHLS"
     gene            8807..10252
                     /locus_tag="VF874_13695"
     CDS             8807..10252
                     /locus_tag="VF874_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX9177076.1"
                     /translation="MRRLFSAAVVAVSAALAISGCTNLLAGHPVSIFADPFRVAGLPA
                     TDGPSGLRPDAAEPTRQVEGTDGGKEDELAVQSVSDVEEFWDGAYRPPLEGNFQPASA
                     VVSWDSTEYSTQQFCGNDTYDFVNAIYCWDDNNIGWDRGQLLPMLRKEFGDISIPLVL
                     GHEYGHAVQRRAQLAGTATPSLVAEQQADCFAGVYMRWVAEGKSPRFTMSTGEGLNSV
                     LATVISLRDPVQSEGESDSDGDEHGSAFERISAFQFGFTDGAGSCVAIDEDEIKQRRG
                     DLPVALTQDTTGEWPVSEESVKAIVDAMTLTFSPAKPPSLSFDTSDADTCADARPSPP
                     ASYCPATNTITVDLPQLQEMGTPSEERGGFGLPLGDNTAYSVLVSRYMLAIQKQKQPS
                     VVLDNAAAALRTACLTGVATTKMSQQINVDGHTVQLAAGDLDEAVSGMLTNGLAASDV
                     NGESVPSGFSRIDAFRAGVLGDEDRCFQRYP"
     gene            complement(10318..>10691)
                     /locus_tag="VF874_13700"
     CDS             complement(10318..>10691)
                     /locus_tag="VF874_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HEX9177077.1"
                     /translation="GYQIGCGIDMSTSNGVTLGGSAGMTSSIGTTGPFIGPPTGLLPI
                     VSFPVSGAITIGLKPGIVNIVPVDKKEFKGDEPWVMISNFHVKIDGCVGQSFIRSYAT
                     LTRQTEESDAVLSWMGVTKTV"
BASE COUNT         1776 a         3678 c         3497 g         1740 t
ORIGIN      
        1 gttaccaaag tcgatgacca acggcggagc gcccggcgct gtcagcaggt aacccgacgc
       61 cggcgaatca ggtccggtga cgctgcccga gcacccgagc acggtgattc gcacagacct
      121 agcttgccac gccgcaagtg ctgttgacga aaacattgca gcaaaacgct cgcgagtaag
      181 tgggggagtc tcatgacgag gcgccaacgt gacgccggac aggtcgaaca ccgtccagcg
      241 ctggtcccag gaagcgcgcc gcgagcttcg tgaacgcctc cggatcaccc gtcgcttcaa
      301 agatccgggt ggctggcggg gcgtcgtgcg gacgcagcag atctaactcg gtgagcacgc
      361 gcacgagatc cttcgcggtc tcctcggcgc tggacaccaa cgtgaccgcc tcacccatcg
      421 ccagttggat cagccccgac agcagcgggt aatgggtgca acccagcacc aaggtgtcga
      481 tgcccgcgcg ctgcagcggc tccagatagc cctcggccag gccgagcacc tgccgcccgc
      541 tggtcacgcc gcgctcgacg aagtcgacga accgcgggca ggccacgccg gtgatgacgg
      601 tgtcgcgggc cgcggcgaac gcgtcctggt aggcgcccga cgcgatcgtc gcctcggtcc
      661 cgatgacgcc gatccgcccg ttgcgtgtgg tcacgacggc ccggcgcacc gcgggcagga
      721 tcacctcgac gaccggcacg tcgtagcgct cgcgcgcgtc gcgcaagcac gccgaagacg
      781 cggtgttgca ggcgatcacc aacgccttca cgccgtgagc caccaggtcg tcgccgatcg
      841 ccagcgcgtg ggcccgtacc tccggaatgg ccaacggacc gtacgggccg tttgcagtgt
      901 cgccgacgta gatgacgtct tcgtcgggca gctggtcgat gatcgcgcgc gcgacggtca
      961 gcccgccgac gccggaatcg aagatgccga cgggagccaa tgcggatgtc acgacgtcag
     1021 gtatggcggc gtcttccctc ggcgcagttc gcgttgcttg cgctccggtc cggaaagcac
     1081 atatgcggcg aacacaccgg cgatccctcc gcacaggtgg ccttgccagg agatgcctgt
     1141 gcagccgacg accggcaagg cgcccaggag gatcgtgccg tagaagaaaa gcacgaccag
     1201 cccgacaacg atctcccaga cgtttcgcgt gaaccacccg aacaccagca ggaacgtcag
     1261 ccatccgaag atcagtcccg acgccccgac gatgttggtg ttaccgccgc aagtgttgcc
     1321 gacatcgccg atcagccaag tgccaaagcc cccgacgatc cagatgatcg ccgtggcgaa
     1381 gacgaaccgc gacaagccgg ccaacgtcat caagaagccc agcaccagcg ccggcagcgt
     1441 gttggccatc aggtgctgcc aattcgcatg cagcatcggc gaaaacagaa tcccccacaa
     1501 cccgtcggat tgcagcggcc tgatgccgtt gtcttcgagg cggtcgccac tcatctggtt
     1561 gaacacctcg atgacgtaga gcagcgccac gaaggtcagg atcgtgatgc cgccggtcag
     1621 ccacgccggc cgcttcttcg gcgcgggctt cgtggaatag tccggcccga tcatgcccat
     1681 aactgccctt ccagcgcgtc ctcggcttcg tccaggctac cggcgtacgc gccggtcgac
     1741 agatatttcc acccggcgtc ggccaccacg aacgcgatgt cggcgcgttg cccggccttc
     1801 agcgccttgt tcgccatgcc cagcgcggcg tgcagcacgg cgccggtgga tatcccggcg
     1861 aaaatgccct cggcagaaat caattcgcgg gtacgccgga ccgcgtccgc agagccgacc
     1921 gagtagcgag tggtcagcac gtcggggtcg tacaactcgg ggatgaaccc ctcgtcgatg
     1981 ttgcgcaacg cgtacacgcc ttcgccgtac cgcggttccg cggcgacgat ctggatgtcc
     2041 ggcacgtgct cgcgaagaaa ccgcccggtg cccatcagcg tgccggtagt gccgagcccg
     2101 gcgacgaagt gagtgatctc gggcaagtcg gcgagcagct ccgggccggt gccctcgtag
     2161 tgcgccgcgg tgttggcctg attgccgtac tggtagagca tcacccacga cgggttcttc
     2221 gcggccagtt ccttcgcgtg ggccaccgcg gtgttcgagc ctccctcggc gggcgagtag
     2281 atgatctgcg ctccgtacag ctcgagcagc tggcggcgct cgatcgacgt gttttccggc
     2341 atcacgcaga tgagcgtgta gcccttgagc agggcagcca ttgcgagcga gattccggtg
     2401 ttgccgctgg tcggctccag gatcgtcgcg cccggtttca gcagcccgtc gcgttcggcc
     2461 tcctcgatca tccgcagcgc gggacggtcc ttgatcgagc cggtggggtt gcggtcctca
     2521 agcttggccc acagccggac gtggtctggg tcatcccact tcggcgacag ccgctgcagc
     2581 ccgaccagcg gggtgttgcc cagggcttcg agcagcgagt cgtagcgagt caagctgcgg
     2641 ccttctgctc ttcgcgcaag cggctcatcg ccctcccgct acggccggca ggatcgtcac
     2701 ggtgtcgccg tcgttgatct cggtgtcgag cccgccggag aaccgcacgt cctcgtcgtt
     2761 gatatagatg ttgacgaagc ggtgcagctt gccgttatcg atgagtcgct ccgagatgcc
     2821 ggagtagttc gcctccagat cggagatcac cgcctgcaac gtgtcccccg cggcgttgac
     2881 gcgcttctca ccgccggtgt ggctcctcag aatcgttgga atcgacacgc tgaccgtcat
     2941 ttgtgggctc cttctggggc gctttcgtag ttgtcgacga cgctcaccgg ctcctcggtg
     3001 accacggcgt cgacgatccg gaaactgcgc aactcgtggc gacatggatc gcgggtggac
     3061 accagcacgt agtgcgcgtc gggctcggcg gcgagcgaga cgtccgtacg gctcgggtac
     3121 gcctcggtgg cggtgtgcga gtggtagatc accaccggaa cctcgtcggc ctcatccatc
     3181 tgccgccaca ctttgagctg ttcgcccgag tcgaagcggt agaacgtcgg cgaccgctcg
     3241 gcgttgacca tggcgatgtg acgcatcggc cggtcggatc cctcggggcc cgcgagcacc
     3301 ccacacgcct cgtcggggtg atcggcccgg gcgtgggcca cgatggcgtc gacaaggtcg
     3361 gcgcggatga tcaacacttc attgctcctc gcgattgctc ctcgcatccg cagaacgcca
     3421 catccccttt cacgcgaatg cccccggcaa cacgtcacga taggtgggta ttccggcgac
     3481 cgcgctcgcg gaggtcaccg ccagcacgac ggcgcgggcc aggcagtcgg cggcggccgc
     3541 gccgacggca cccgtgacct cggcctgctc cgacatcgcg gcggggagct tcccgctcgg
     3601 cggcgtctcg atcgcgccgg tggccacgac gaacaccgtg tcgccgtcca atggagagtg
     3661 cgaggggcgg attgcgcgcg ccaatccgtc ggcggccatg atcgcgaccc gccggcacga
     3721 tggcggggag agcatcgcgt cggacgccac cacggcgatg gtgctgttga acgcctgcgc
     3781 gtagcgatcc cgttcggcgg ccagcgcagc gaaggctgcg acctgagccg gtggcggcgg
     3841 cgccagtccg agctcgctgg tgaggtagtt ggtccagggc aatccggtgg cggggtcgac
     3901 gacgtcgccg acgcagttca ccacaaccaa cgcgccgacc gtgacacccg agcgcagcac
     3961 gctcgacgcc gtaccgacac cgcccttcag cactcctgca cgtgcgcccg tacctgcgcc
     4021 caccgacccg acgcccacgt cacgtcccgc gccttgcgcc gcggcgtaac cgaactcggc
     4081 cgtagggcgg tagttccagt cgcccactgc gaggtcgtag atgaccgcgc tcggcacgat
     4141 cggcaccacg cgctcgccga caccgtcgtc catcacgatg ccgcgacggt gttcctccag
     4201 ccactgcatg actccgtccg cggcggccag cccataggcg ctgccgccgg tcagaaggat
     4261 cgcgtcgacg tagcgcactg tgttggcggg atcgagaagg tcggtctccc gggtgcccgg
     4321 cgcgcccccg cgaacttcga ccgcggcgac ggtgcctgca ggggtcagca ccaccgtgct
     4381 gccggtggcc cagccagagt ggtccagccg gtgatggtgg ccgaccgtaa cgccgccgac
     4441 gtcggtgatg gacccggtcg tcgaaatcat ggcgccatca gcgttgcccc atcagcgcca
     4501 acaccaggta ctcctgcatc accgtcagcc actgatagac gtcgaaatgc ccggccatcg
     4561 ggtggtcgtc aggcatccgc tccggaccgc tggggccgat gccgagcatc gtgcccagcg
     4621 ccaaccggat gtcgttgacc gccgcgaccc acgcgttggc gtcctcgcga gtcaactcga
     4681 accggccgcc accgacaggc accgtgcgca gcattgtttg gccggcttcg cgcttggctt
     4741 cgatgatctc cggctcgtgc agactgcgca gcgccgcgtt caggctttcc gcggcggccg
     4801 aacccgccgg gtggttgcgt tcggtgcggt agaagtcggg cagtagccgg cgcatcgtcg
     4861 cgtcctgcgg acgccgtgga ttccccgctt tgatgccggt gatcttctcg agttcatctg
     4921 caggggttga ggattcgcgc tcctcgagca tgtccagcat cgacttcacg agactgtgca
     4981 gcatcgaggc ctcatgtgcg gccaacgccg accggaagcg ctggccatca gcggtgtcta
     5041 cccgcttcca tttgcgcacg cgtccgtcgt cctaggccgg tcagcggtcc tgctgcagcg
     5101 tggcccacag cccggcggca tgaagtttgg acacgtcggt ttccatggct tcgcggctgc
     5161 ctgcggacac cactgccttg ccctcgttgt gcacctgaag catcagcttg gtggcgtgcg
     5221 gctcgctgta accgaacagc ttctggaaca cgtaggtcac gtaggtcatc aggttcaccg
     5281 ggtcatccca cacgagggtg acccatggca cgtccgtagc ctcgacgaca tcgacgtcga
     5341 cttcctcgcg agtcccaggg cgggtccgcg caggagtaac catgtgccaa gaataccgac
     5401 ggacaccgac agacattccg tccgccttgt cgccaccagc cgatacggtt aacggtgtga
     5461 cgactccgtc ggagccaacc gcgctgctga ccgacaagta cgagctgaca atgctctcag
     5521 cggccctgcg cgacggcacc gcgcaccggc acaccacgtt cgagctgttc gcccggcgcc
     5581 tgccggaagg ccgccgctac ggcgtggtgg ctggcaccgg ccggttcctg gaagcgttgg
     5641 cgtcgttcga gtttgacgac gtcgcactcg actcggtgcg cggattcctc gaccccgaca
     5701 ccgtcgacta cctcgcccgc tatcgattcc gcggcgacgt cgacggatac gccgagggcg
     5761 agctctactt cccgggatcg ccggtcttga cggtgcgggg caccttcgcc gaaagcgtgg
     5821 ttctcgaaac gctcgtgttg tcaatcctca accacgacac cgcaattgct tcggcagcgg
     5881 cgcggatggt cagtgccgcc gacggccgtc cactcatcga aatgggttcg cggcgtgctc
     5941 atgagcacgc ggcggtcgcc gcggcgcggg ccgcatacat cgccggcttc gccggatcgt
     6001 cgaaccttgc ggcccagcgc cgctacggtg tcccggcgct gggcaccagc gcgcacgcgt
     6061 tcacgttgct gcacacaacg gccgacggtc cagacgaaca ggcagcgttc cgcgcgcagg
     6121 tcgacgcgct cggggtgaac acgacactgc tggtcgacac ctacgacatc accgcgggcg
     6181 tcgcgaacgc ggtggccgtc gccggtacgg cattgggcgg ggtccggatc gactcgggcg
     6241 acctcggcgt actggcccgc caagttcgcc gccagctcga cgacctcggc gcgcggcaca
     6301 cccggatcgt cgtgtccggc gacctcgacg agttcgcgat cgctgcactg cgcgcggagc
     6361 cggtggacag ctacggtgtc ggcacgtcgg tggttaccgg gtccggtgcg cccaccgcga
     6421 acatggtgta caagctggtc gaggtcgacg gaatgccagt ggaaaaacgt agcagccgca
     6481 aggaatccca cggcgggttc aagcacgcga tgcggttggc acggccgacc ggaaccattc
     6541 tggaggaagt cgtctatccg gcggggcggc caccggccgt cgccgaggcc ggccgagagt
     6601 tgagcgtgcc gttggttcgc ggcggtgacc cgttggtcga cgacaacctc accgccgtgc
     6661 gggcacgcgt ggccgctgga ctcatgagcc tgccgtggga cggcctgaag ttatcccgcg
     6721 gcgagccggc gattccgacc aggatggtgc cggccaccat cgaggagggg ccacgccagc
     6781 ggccgcggtg accgacctgc tcgccacggc cgtcgacgct ctcgggggca gtgaacgggc
     6841 cggtcaggtc accatggccg aagcggtggc gcacgcattc gacaccggcg agcatctggc
     6901 cgtgcaggcg ggcaccggca ctggaaagtc cctggcctac ctcgtgccag caatcgctcg
     6961 cgccgtcgaa accgattgtt ccgtcgtggt ttccaccgcc accattgcgc tgcaacgtca
     7021 gctcgtcgaa cgcgatctgc cgcggctggc cgactcgctg gaacacctcc tgcctcgccg
     7081 gcccagcttc gcgatcctaa agggccgggg aaactatctg tgcctcaaca agattcacaa
     7141 cggctcagct tcggacgcaa gcgacgatgg tgacgacgca ttgttcgaac cgaccgcgat
     7201 cacggcgctg ggccgcgacg ttcagcggct gacgtcgtgg acatccacca ccgacaccgg
     7261 cgaccgcgac gagctgaggc ccggtgtccg tgaccggtct tgggcccagg tgagcgtctc
     7321 ggcacgagaa tgcctcggcg tggcgcgctg cccgttcggc gcggagtgct tcgccgaaaa
     7381 ggcacgagga cgcgccggct gtgctgatgt ggtcgtgacc aaccacgcgc tgttggccat
     7441 caacgcaatc tccgatgcgc ccgtcctccc cgaacacgat ctgctcgtga tcgatgaggc
     7501 ccatgagctg gtggacaggg tcactgccgt tgcgacgcaa gagctttcgt caacacccct
     7561 ggcagttgcc gaacggcgga ttgctcgctt ggtggagtct gagctggccg agcgcttcga
     7621 cgcggcagca accacgttca gctcggcgat ccgggacgct gcacccggcc gcatcgacca
     7681 ccttgacgac ggtctcgcca cgcatctcgc tgcgctcagg gatgccgcca ccgccgcccg
     7741 tcgcgcgatc ccgacgacgt ccaccgatcc gaaggcaggt gccgcacgct cggaggccat
     7801 tgccgcgctc gacgacatcg ccgacacctg cacgcggatc ctggagtcgt ttcaggccgc
     7861 tgttgccgat cggtccgacg tcgtctggct ggtcaacgac gacagccgcg gcctggcgca
     7921 actgcgcgtg gcaccactgt cggtggcggc gctgctgagc ggcgggttgt tcaaccacgc
     7981 gaccgcggtg ctcacgtcgg cgaccctcac gcccggcggg tcgttcgacg cgatcgcgtc
     8041 cgactgggga ctcaccggcg ctgatgccga cttccggtgg cgtggcatcg acgtgggatc
     8101 gccgttttcg catgcgaagt cgggcatcct ctacgtcgct cgccacctgc cgccgccggg
     8161 cagggacggc accggctccg ctgagcaact cgccgagatc gaggcgctgg tctccgccgc
     8221 ccacggccgt acgctgggcc tcttctcgtc gatgcgtgcg gccgccgcgg cagctgaaga
     8281 gatgcgcgag cggctcacca caccggtgct gtgccagggt gacgacgcca ccgcggcgct
     8341 cgtcgagcgg ttcgcggctg aacccgaaac atctttgttc ggaacgcttt cgctgtggca
     8401 gggggtggac gtgcccggac cgtcgctgtc gttggtgatc atcgaccgaa ttccgttccc
     8461 tcgtcccgac gatccgctgc tcaccgcgcg acaacgggcg gtaaatgccc gcggcggtaa
     8521 cggtttcatg gccgttgccg ccacccacgc cgcgctgctg ctcgcgcagg gtgcgggccg
     8581 gctgctacgc cgagtggacg acaggggcgt agtcgccgtt ctcgactccc ggatggcgac
     8641 ggctcgctac ggccgattcc tccgagactc cctgccgccg ttttggttca ccaccgaccg
     8701 ccacaccgtg ctggcagcgc ttgaccgact gacggccggc ccagacggag gccatctatc
     8761 ctgacagcca actgggtccc ttctggaccg gccgcgaaag gtagccatga gacgactgtt
     8821 cagcgccgcc gtcgttgcgg tcagcgccgc cctggcgatc agcgggtgca cgaacctgct
     8881 cgcggggcat ccggtctcca tcttcgctga cccgttccgg gtggccggcc tgccggcgac
     8941 cgacggccca tcgggattgc gaccggatgc cgctgagccg acccggcagg tggaaggcac
     9001 cgacggcggc aaagaggacg aactcgccgt ccagtccgtc agcgatgtcg aggagttctg
     9061 ggacggcgca taccgccctc cgttggaggg caacttccaa cccgccagcg cggtggtgtc
     9121 gtgggactcg accgagtaca gcacccaaca gttctgcggc aacgacacat acgacttcgt
     9181 caacgcgatc tactgctggg acgacaacaa catcggctgg gaccgtggac agcttcttcc
     9241 catgcttcgc aaggagttcg gtgacatctc gatcccgttg gtgctcggcc acgaatacgg
     9301 gcacgcggtc cagcgccgcg ctcaactcgc cggcacagcg acgccctcgt tggtcgccga
     9361 gcaacaggcc gactgctttg cgggcgtgta catgcgctgg gtggccgagg gcaagtctcc
     9421 gcgattcacg atgagcaccg gggaggggct gaacagcgtt ctcgctacgg tcatttcgct
     9481 tcgcgacccg gtgcagtccg aaggcgaatc ggattcggac ggcgacgaac acggctcggc
     9541 cttcgaacgg atctcggcct ttcagttcgg tttcaccgac ggcgccgggt cgtgcgtcgc
     9601 catcgacgaa gacgagatca agcagcgccg cggagatctg cccgtcgcgc tgacccagga
     9661 cacgacgggt gaatggccgg tcagcgagga atcggtgaaa gcgatcgtcg acgcgatgac
     9721 gctgacgttc tcccccgcga aaccaccgag cctcagcttc gacacgtccg acgcagacac
     9781 atgtgccgac gcgcgcccga gcccgccggc gtcctactgc cccgccacca acacgatcac
     9841 cgtggacctg ccgcaactcc aagagatggg caccccgagc gaagaacgag gtggtttcgg
     9901 gctgccacta ggcgacaaca ccgcctactc cgtgctcgtg tcgcggtaca tgctggccat
     9961 ccagaaacag aagcagccca gtgtcgtgct ggacaacgcc gcggcggcgc tgcgcacggc
    10021 ctgcctgacc ggtgtcgcga ccaccaagat gtcgcaacag atcaacgtcg acgggcacac
    10081 ggtccagctg gcggccggcg acctcgacga ggcggtttcg ggcatgctca ccaacggtct
    10141 cgccgccagc gacgtcaacg gcgagtcggt gccctcgggg ttctcgcgca tcgacgcgtt
    10201 ccgtgccggg gtgctcggcg acgaggaccg ctgcttccag cggtatccgt gatgagcctc
    10261 tcgggcgaag aatcaaaagg ctgatgagac tctcgggcga agaatcaaaa cgcagcgtca
    10321 gacggtcttc gtgacgccca tccacgacag taccgcgtcg gactcctcgg tctgacgggt
    10381 cagcgtcgca tacgagcgga tgaacgactg accgacgcag ccgtcgatct tgacatggaa
    10441 gttggagatc atcacccacg gctcgtcgcc cttgaactcc ttcttgtcca ccgggacgat
    10501 gttgacgatg ccgggcttga gcccgatggt gatcgcgccg ctgaccggga acgacacgat
    10561 cggcagcaat cccgtcggcg ggccgatgaa cggcccggtc gtgccgatgg acgacgtcat
    10621 gcctgcggag ccgcccaggg tgacgccatt cgaggtgctc atgtcgatgc cgcagccaat
    10681 ctggtagccc a
//
LOCUS       DASWBF010000366         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1019067,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000366 DASWBF010000000
VERSION     DASWBF010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019067"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VF874_13705"
     CDS             <1..713
                     /locus_tag="VF874_13705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX9177078.1"
                     /translation="ARAGFDTLLVTVDVPVSGARLRDIRNGLTIPPTLTARTVVDALR
                     RPRWWIDLLTTEPLMFATLDRWPGTVAELLDSMFDPTITFGDLAWIKSQWPGKFVVKG
                     IQTIEDARRVVELGVDGIVLSNHGGRQLDRAPVPFHLLPSVATEFGSDVEIVLDTGIM
                     SGADVVAAVALGARFTLVGRAYLYGLMAGGRAGVDRAIEILAGQLTRTMKLLGVTCLE
                     ELSPRHVTQLVRSVPRSR"
     gene            complement(737..1015)
                     /locus_tag="VF874_13710"
     CDS             complement(737..1015)
                     /locus_tag="VF874_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1876 domain-containing protein"
                     /protein_id="HEX9177079.1"
                     /translation="MYDRNLDNIWPVEIKFVEDETHTHATARAALRGGETIAATGDAY
                     RNPTDASRPMIGEEIAAARALISLGSALLQAASMRIEQATQRPVHLHG"
     gene            complement(1089..>2034)
                     /locus_tag="VF874_13715"
     CDS             complement(1089..>2034)
                     /locus_tag="VF874_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9177080.1"
                     /translation="LDVVVVEKRCGPIDKACGEGLMPHAVAQLDHVGVTAKGKAFHGI
                     SYLDGTRCVTAAFRSGAGRGVRRTALHAALLEAATKAGVRMVQDEVGEVTQDANSVRC
                     GNFRARYLAAADGLHSPIRASLGLASSPSGRRRWGIRRHIQITPWSDNVEVYWSDDAE
                     AYVTPVSDECVGIAVLTSRRGGFGQHLDGFPVLKDRVDGHPHGPDRAAGPLRQKVKNR
                     RAGRVLLVGDAAGYVDALTGEGLGISFSAAELLVDCVVAERPADYDRQWRRMSRRYRF
                     LTAAVLHASAIPPVRSCIVPAASRLPNVFAQLVNRLAE"
BASE COUNT          301 a          687 c          699 g          347 t
ORIGIN      
        1 cggcccgtgc cggtttcgac acgctgctcg tcaccgtaga cgtgccagtg tccggggccc
       61 ggctgcgaga catccgcaac ggcctgacga ttccgccgac gctcacggct cgaacggttg
      121 tcgacgcgct gcggcggccg cggtggtgga tcgacctgct caccaccgag ccgttgatgt
      181 tcgcgacgtt ggaccgctgg cccggtaccg tcgcggagct gctggacagc atgttcgacc
      241 cgaccatcac cttcggcgac ctggcctgga tcaagtcgca atggccggga aagtttgtgg
      301 tcaaaggcat tcagaccatc gaggacgccc gccgggtggt agagctcggc gtcgacggga
      361 tcgtgctgtc caaccacggc ggccgtcagc tggaccgggc gccggtgccg tttcacctgt
      421 tgcccagcgt cgcaacggaa ttcgggtccg atgtcgagat cgtgctggac accggcatca
      481 tgtccggggc cgacgtggtc gcggccgtcg cactcggcgc gcggttcacg ctggtcgggc
      541 gcgcctatct gtacgggctg atggccggcg gccgagccgg tgtcgaccgc gcgatcgaga
      601 tcctcgccgg ccagctcacg cggaccatga aactgctcgg tgtgacatgc ctggaggaac
      661 tgtccccgcg gcacgtgacc cagctggtcc ggtcggtccc gcggtcacgg tagcgcgggc
      721 agcgccacgg cggcggtcag ccgtgcagat gtaccggtcg ctgcgtggcc tgctcgatcc
      781 gcatcgacgc ggcctggagc agcgcgctgc ccagcgagat cagcgctcgg gccgcggcga
      841 tctcttcgcc gatcatcggc cggctggcgt cggtcgggtt gcggtatgcg tcccctgtgg
      901 ccgctatcgt ctctccgccg cgcagcgcag cccgcgccgt cgcgtgggtg tgggtctcgt
      961 cctcgacgaa cttgatctcc acgggccaga tgttgtccaa attcctgtcg tacattgcca
     1021 tcaccttccg tcgaacagag tgcgccgatc acggcccgcg caccagagca gaaatgactg
     1081 gacggcgcct attcggcgag tcggttgacg agctgggcga agacgttcgg tagccgcgag
     1141 gcagctggca cgatgcacga gcgcacgggt ggaattgcgc tggcgtgcaa caccgctgcg
     1201 gtgaggaagc ggtagcggcg cgacatgcgg cgccactggc ggtcgtagtc ggccggccgc
     1261 tcggcgacga cgcagtccac caacagctcg gcggcactga aggagatgcc gaggccttcc
     1321 ccggtcagcg cgtcgacgta tcccgccgcg tcaccgacca ggagaacacg cccggctctt
     1381 cgatttttca ccttctgtcg caacgggccc gcagcgcggt cgggaccgtg tgggtgcccg
     1441 tcgacccggt ctttcaggac tgggaatccg tcgaggtgtt ggccgaatcc gccccggcgc
     1501 gaggtcagga ctgcgatgcc gacgcattcg tcggacaccg gggtgacgta cgcttcggcg
     1561 tcgtcggacc agtacacctc gacgttgtcg ctccacggcg tgatctggat gtggcggcgg
     1621 attccccacc ggcgccggcc gcttggcgac gaggccaggc cgagagacgc gcggatcggc
     1681 gagtgcagtc cgtcggcagc ggcaaggtag cgggcgcgga agttgccgca gcgaaccgaa
     1741 ttcgcgtcct gagtcacctc gccgacctcg tcctgcacca tccgcacacc ggccttcgtc
     1801 gccgcctcca ggagcgctgc gtgcaaggct gtcctgcgca cgccgcgccc ggctcccgat
     1861 cgaaatgccg cggtgacaca ccgcgtgccg tcgaggtagc tgatcccgtg gaacgctttg
     1921 cctttcgcag tgacgccgac gtggtcaagt tgtgcgaccg cgtgtggcat cagcccttcc
     1981 ccgcaggcct tgtcgattgg cccgcagcgt ttttcgacaa caacgacgtc gagt
//
LOCUS       DASWBF010000367         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_161241,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000367 DASWBF010000000
VERSION     DASWBF010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161241"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..2365)
                     /locus_tag="VF874_13720"
     CDS             complement(224..2365)
                     /locus_tag="VF874_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9177081.1"
                     /translation="MFWNRLAAVVTARRSWLIAIAVALLGGALIGLAGANEAGTQSPE
                     FLPTSAEAARAAAALKQFPGGDQAAAILVVTRTDSAVLSPDDLSAVGSAAAPSPVMTS
                     DDGRAAIATLPLAANLSGFALNDAVESLRNSAADRLPDTLTAHVTGGPAFGADIANSF
                     SGANVTLLIVTALVVAVLLIVTYRSPVLWLVPLAVVGFADQVATAVGTAVAGATGLTF
                     DGSTSGITSVLVFGAGTNYALLVISRYREELRHDDDPRQALRRAVRAAGPAIVASNAT
                     VVLALLTLLLAIVPSTRSLGALAASGLVVAAVFVLLVLPPVLALFGTRLFWPFVPRPG
                     TPDTVDTGPWHRVAKAVARRPALIGGVAVLGLAVLASGLLGTKIGLSQTEQFRVKADS
                     VSGFDTLATHFPGGLTDPAIVVADSGRADAVQQAIDTTPGVVSATPAGQSPTGLSRWS
                     VIIDAAPASGKALETIDALRNSVHAADPGALVGGSDAQARDVRDAAARDRLVVIPAIL
                     AVVLLVLYVLLRAVLAPPVLVAATVLSALAALGLGGWASIHVFGFPALDNSTPLFAFL
                     FLVALGVDYTIFLVTRAREETPEHGTRDGMVRAVSATGGVITSAGVVLAAVFCVLGVL
                     PLIVMTQLGIIVGLGILLDTFVVRTLVIPALFALIGPAIWWPSLSERPGRPPPLEPAA
                     AAKSAPTANDSGYRRQGTSLPPSGHGRHRRQ"
     gene            2430..>2627
                     /locus_tag="VF874_13725"
     CDS             2430..>2627
                     /locus_tag="VF874_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9177082.1"
                     /translation="MTTTGPVSRAELEEQISADIRALSAESDQIGRLFASRHDLSPND
                     FRALLHVMVADTAGSPLTSGEL"
BASE COUNT          453 a          923 c          895 g          356 t
ORIGIN      
        1 gccatggtcg gcgatccgct gtggggagtg ggctgcggct gggcgtttca gtccgcggaa
       61 tggctcagcg acgccgtcgc accggtcctg ccctgtggct cgacccacga aatcgattcc
      121 gccgccaggc gataccagcg caagcaccgg cgcaagctac gcctgcacca gctcctgtgc
      181 atcgacttca gtcagcacac cggtgtcaac ccgctggtcg atctcattgt cgccggtgcc
      241 gtccgtgacc cgaaggtggc agagaagttc cttgacgtcg gtacccggaa tcgttcgccg
      301 ttggcgctga tttcgccgct gctgctggct cgagcggcgg tggccgcccg gggcgctccg
      361 acagcgacgg ccaccagata gccggcccga tcaacgcgaa cagcgcggga atcaccagcg
      421 ttcgcacgac gaatgtgtcg agcagaatac ccagtcccac aatgattccc agctgtgtca
      481 tgacgatcag cggtagcacc ccgagcacgc agaacaccgc ggccagcacg acgcccgcgc
      541 tggtgatcac accgcccgtc gccgacaccg cgcgcaccat tccgtcgcgc gtcccatgct
      601 cgggcgtttc ttcacgcgcc cgggtcacca ggaagatcgt gtagtcgacg ccgagcgcca
      661 ccaggaacag gaacgcgaac agcggcgtgc tgttgtcgag cgcgggaaag ccgaacacgt
      721 ggatgctggc ccatccgccg agcccgagcg cggccaacgc ggacagcacg gtggcggcga
      781 cgagcacggg cggcgcgagc accgcgcgca acagcacata cagcaccagc agcacgaccg
      841 cgaggatcgc gggtatcacc accagccggt cccgtgcggc ggcgtcgcgc acgtcgcggg
      901 cttgcgcgtc cgatccaccg accagtgcgc ccgggtccgc ggcgtgcacc gaattgcgca
      961 gcgcgtcaat cgtttccaat gccttaccgg atgccggtgc ggcgtcgatg atcaccgacc
     1021 agcggctcag gcccgtcggc gactggccgg caggggtagc ggacacgaca ccgggtgtgg
     1081 tgtcgatcgc ctgctgcacg gcgtccgctc ggccgctgtc ggcgaccacg atcgccgggt
     1141 cggtcaagcc gcccgggaaa tgcgttgcga gcgtgtcgaa tccgctcacc gaatcggcct
     1201 tcacgcggaa ctgctcggtc tgcgacagcc cgatcttggt gcccagcagg ccacttgcca
     1261 gcaccgccaa ccccaggacc gcgacgccgc cgatcagagc cggtcgccgc gcgaccgctt
     1321 tggcgacacg gtgccacggg cccgtgtcga cggtgtccgg ggtgccgggc cgcgggacga
     1381 acggccagaa cagccgggtg ccgaacagcg ccagcaccgg cggcagcacc agcagcacga
     1441 acacggcggc gaccaccagt ccggacgccg ccagcgcgcc gaggcttcgg gtgctgggga
     1501 cgatcgccag aagcaacgtc agtagcgcca gcacgacggt ggcgttgctg gccacgatcg
     1561 ccggccccgc cgcgcgcacc gcccggcgca gcgcctgacg gggatcgtcg tcgtgccgca
     1621 actcttctcg gtatcgggag atcaccagca gcgcgtagtt ggtgccggcc ccgaacacca
     1681 gcacgctggt aatgccggac gtcgagccgt cgaacgtcag cccggtcgcg ccggcgaccg
     1741 ccgtgcccac cgccgtggcc acctggtcgg cgaacccgac gacggccagc ggtaccagcc
     1801 acagcaccgg cgagcggtac gtgacgatca gcagcaccgc gaccaccaac gcggtgacga
     1861 tcagcagagt gacgttcgcc ccgctgaacg agttcgcgat gtcggcgccg aacgcggggc
     1921 cgccggtgac atgggcggtc aacgtgtccg gcaaccggtc ggccgcggag ttgcgcagcg
     1981 actcgaccgc gtcgttgagc gcgaagccgg acaggttggc ggcgaggggg agcgtcgcga
     2041 tggcagcgcg gccgtcgtcg gacgtcatca ccggcgatgg cgcggcggcc gacccgaccg
     2101 cggacaggtc gtcgggactg agcaccgcgc tgtcggtgcg ggtgacgacg aggattgccg
     2161 ccgcctggtc gccgcccggg aactgcttca gcgcggcggc ggcgcgtgcg gcttcggcgc
     2221 tggtgggcag gaactcggga gactgagtgc cggcctcgtt ggcgccggcg aggccgatca
     2281 acgcgccgcc gagcaacgcg acggcaatcg cgatcagcca cgagcgacga gcggtgacca
     2341 ccgcggcaag gcgattccag aacaccgtca gataatttca gatacttaac agttaatcaa
     2401 atgaacttgc tcccgatatg ctgctgtcga tgacgacgac gggcccggtc tcccgcgcgg
     2461 aactggaaga gcagatctcc gccgacatcc gcgcgctgtc cgcggagtcc gaccagatcg
     2521 gtcgcctgtt cgcatcgcga cacgacttga gccccaacga ctttcgcgct ctgctgcacg
     2581 tgatggtcgc tgacactgcg ggcagtccac ttacgtcggg cgaactc
//
LOCUS       DASWBF010000368         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_363895,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000368 DASWBF010000000
VERSION     DASWBF010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363895"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VF874_13730"
     CDS             complement(<1..146)
                     /locus_tag="VF874_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HEX9177083.1"
                     /translation="MTAPEADLTGWTAAPFTAEGITHDVYRKGEGPGVVLIPEIPGLT
                     PQVLA"
     gene            complement(173..847)
                     /locus_tag="VF874_13735"
     CDS             complement(173..847)
                     /locus_tag="VF874_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003915494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HEX9177084.1"
                     /translation="MTSQPGPVPREPKPRLLQDGRDMFWSLAPLVVACIVLAGLAGMC
                     SFQARGPKTGPAPEYDAPAALRADAAQLGFPVRLPQLPDGWHANSGRRGSIEQGRKNP
                     ATGTWERAVISTVGYLAPSGMYLSLTQSNADEQKLVEEIYSGVVPTGAQNVAGAAGPA
                     TSGTVVSWVVYEGGEGTRPVWTTRLDGPTGPAQIAITGAASPDEFRTLAAATQSQQPL
                     PATVGN"
     gene            901..1932
                     /gene="glpX"
                     /locus_tag="VF874_13740"
     CDS             901..1932
                     /gene="glpX"
                     /locus_tag="VF874_13740"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX9177085.1"
                     /translation="MPEPRRREAPDRNLALELVRVTEAGAMAAGRWVGRGEKEGGDAA
                     AVDAMRELVNSVSMRGVVVIGEGEKDNAPMLYNGEEVGNGDGPDCDFAVDPIDGTTLL
                     SKGLSNAISVLAVSERGTMFDPSAVFYMNKIAVGPEAVDAIDITAPIGENIRQVAKAK
                     NVSVSDLTVCILDRPRHKQLIADVRAAGARCRLITDGDVAGAISACRPNTSTDMLAGI
                     GGTPEGIIAAAAIRCMGGAIQGQLAPKDDDERQMAIDRGYDLDQVLSTEDLVSGDNIF
                     FCATGVTDGDLLKGVHYYSGGCTTQSIVMRSKSGTVRMIEAYHRLSKLNEYSAIDFTG
                     DSNAIYPLP"
     gene            1954..>2303
                     /locus_tag="VF874_13745"
     CDS             1954..>2303
                     /locus_tag="VF874_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX9177086.1"
                     /translation="MTDTAVEKATDADEYRIERDTMGEVRVPKDALWRAQTQRAVENF
                     PISGRGLERAQIRALGLLKGACAQVNKDLGLLNADKADAIIAAAAEIADGQHDDQFPI
                     DVFQTGSGTSSNMNT"
BASE COUNT          388 a          803 c          758 g          354 t
ORIGIN      
        1 gccagcactt gcggagtcaa gccgggtatc tcggggatca gcaccacgcc ggggccttcg
       61 cccttgcggt acacgtcatg cgtgatgccc tcggcggtga acggggccgc ggtccagccg
      121 gtgaggtcag cttccggtgc ggtcatcggt ctcctatcgc gcgggcctcg ggctaattgc
      181 ctactgtcgc aggcagtggc tgctgcgact gcgtggccgc ggccagcgta cggaattcgt
      241 ctgggctcgc ggcacccgtt atcgcaatct gcgccggtcc ggtcggcccg tcgagccgtg
      301 tcgtccacac cggtcgggta ccttcgccgc cttcgtatac cacccaggag acgactgtgc
      361 cggatgtcgc cggcccggcg gctccggcga cgttctgcgc gccggtcggc acaacaccgc
      421 tgtagatctc ttccacgagc ttctgttcgt cggcgttgct ctgcgtcagg ctgaggtaca
      481 tgccgctggg cgccaggtac ccgaccgtcg agatcactgc gcgctcccac gtgcccgtcg
      541 ccgggttctt gcgcccctgc tcgatgctgc cgcgccggcc ggagttcgcg tgccagccgt
      601 cgggcagctg cggcagccgg accgggaacc cgagctgggc ggcatcggcg cgcagcgccg
      661 cgggcgcgtc gtactccggc gccggaccgg tcttcggccc gcgcgcctgg aacgaacaca
      721 tgcccgccag gcccgccagc acgatgcagg ccaccaccag cggcgccagc gaccagaaca
      781 tgtcgcggcc gtcctgcagc aaccgcggct tcggctccct cgggacgggc cccggctggc
      841 tagtcacggc gctagtatcc cagctggtta tcgaccaccg ccttctggga caatcgccgc
      901 atgcctgaac cccgccgccg ggaagcgccc gaccgtaacc tcgccctcga actcgtccgg
      961 gtcaccgagg cgggcgccat ggccgcgggc cgctgggtag gccgcggcga gaaggagggt
     1021 ggcgacgccg ccgcggtcga cgcgatgcgt gagctggtca actcggtgtc gatgcgcggt
     1081 gtcgtggtga tcggcgaggg cgagaaggac aacgccccga tgctctacaa cggcgaggag
     1141 gtcggcaacg gcgacggccc ggactgcgac ttcgccgtcg acccgatcga cggcaccaca
     1201 ttgttgagca aagggttgtc gaacgcgatc tcggtgctgg cggtgtccga gcgcggcacc
     1261 atgttcgacc cgtccgcggt gttctacatg aacaagatcg ccgtcgggcc cgaggccgtc
     1321 gacgcgatcg acatcaccgc cccgatcggc gagaacatcc gccaggtcgc caaggccaaa
     1381 aacgtgtccg tgtcggattt gacggtgtgc atcctggatc gcccgcggca caagcagctg
     1441 atcgccgacg tccgtgccgc cggcgcccgc tgtcggttga tcaccgacgg cgacgtggcc
     1501 ggcgcgatct cggcatgtcg gcccaacacc agcaccgaca tgctcgcagg tatcggcggc
     1561 actccggaag gcatcatcgc cgcggccgcg atccgctgca tgggtggcgc gatccagggc
     1621 cagcttgcac ccaaggacga cgacgaacgg cagatggcca tcgaccgcgg ctacgacctc
     1681 gaccaggtcc tcagcacaga ggatctggtg tccggagaca acattttctt ctgcgccacg
     1741 ggcgtcaccg acggcgacct tctcaagggt gtccactatt acagcggcgg ctgcacgacc
     1801 cagtcgatcg tcatgcgcag caagtccggc accgtccgga tgatcgaggc ctaccaccgg
     1861 ctgtcgaagc tgaacgagta ctcggcgatc gacttcaccg gcgacagcaa cgccatctac
     1921 ccgctcccct gaccatcaat tagtaaggga accatgaccg acactgccgt cgagaaggcc
     1981 actgacgcag acgaataccg tatcgagcgc gacaccatgg gtgaggtccg ggtacccaag
     2041 gatgcgttgt ggcgcgcgca gacacagcga gcggtggaga acttcccaat ttcgggccgc
     2101 gggctcgagc gcgcgcagat ccgggcgctc ggtctgctca aaggcgcttg cgcgcaagtg
     2161 aacaaggacc tcgggctgct caacgccgac aaggccgacg cgatcatcgc tgcggccgcc
     2221 gagatcgccg acggccagca cgacgaccag ttcccgatcg acgtgttcca aaccggttcc
     2281 ggcacaagct cgaacatgaa cac
//
LOCUS       DASWBF010000369         3505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_243681,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000369 DASWBF010000000
VERSION     DASWBF010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243681"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VF874_13750"
     CDS             complement(<1..637)
                     /locus_tag="VF874_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9177087.1"
                     /translation="MNVDDLILVSVDDHVIEPAHMFEGRVPKKYQDKAPRFVRRDDGT
                     MAWRYEGQEILNTALNAVAGRPPSEFGMEPTCIEEIRPGCYDIHDRVKDMNANGVLGS
                     MCFPSFPRFCGQLFMGTQNRDQAAVMVRAYNDWHIDEWAGTYPGRMIPLALPMMWDPE
                     ATAAEVRRVAAKRCHAVTFSSSPYDLGLPSIYQDHWDPLWRACADEGTVVCM"
     gene            complement(685..2253)
                     /locus_tag="VF874_13755"
     CDS             complement(685..2253)
                     /locus_tag="VF874_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9177088.1"
                     /translation="MTESPESKVERRADDVVWRAIPRRNTQKVSHLLAADLRRQILNG
                     QLAADQQLPSEAELTARLRISRETLREALRILESQQLVEIRRGRGGGAVVRRPGLEAV
                     GRYVALLLQLRRTTLADIEEARSVVEPPAAEQVAIRAGYEDLDSLVALHDAERAAEAD
                     PLSFVTAMTAFDQAVTELSGNRTLAVIAGVFRDIYAGQVYSAIGSDDAVLAERIARRV
                     IVSHSAFLDAARRRDMSLAQKTWSDYLFTTSRMLVSRNVSRQPIDMAPLWRAQARQSG
                     AEPTPRRAAVVATEIRSRIAEGRLGEGDRLPPLAELADEFGISRPTLREALRILEMEF
                     LLDLRTGDRNGATIRTPSTRVAAQLAGIVLEARGTTLADFYRALQLVEPAIMGLVASR
                     IGTKQLKALAVTDSELAATTEDTARFVTKLRETQMLAFAAVKNPALTVIAEILHWVRV
                     GVEPAVTADAKGLPWVSTTNRNTQALFSEFVSAASGRDSARAVDVWTAALEANAPWIE
                     ESELGHRLMLDLMD"
     gene            complement(2250..3407)
                     /locus_tag="VF874_13760"
     CDS             complement(2250..3407)
                     /locus_tag="VF874_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9177089.1"
                     /translation="MSSLTRPVAIAGVGYSHLSRTGAPDPRNLALTAAKVALSDAGLG
                     ATDIDGIFEYKFGPESPGAQDMARLLGVPDLAAFADIMPSNPSGLAGALAGVMAVASG
                     ACETALVFRCLTRAAGHQGGVASGPETVGGRDQFLTPYGYLGGILVLLALKKQRWMAE
                     YGRPEEDFGRIAVNARRWSALNPRAVLRDEVTIEDYLSSRMVADPLRVLDCDYPVNGA
                     AATVITTAERAADLRQPAVLVDAMTYATGWGADWIFDSDFLYGATVDCANRLWQRSSV
                     TLADVDVAQVYDGFTPVTVAWLEALGVCGRGEFGDWVGDGSRIAPGGDFPLNTAGGQL
                     AEGRLHGIGFLNEAVLQLRGQCEDRQVPDARVAVVTSGVYPQCGAMVLTAS"
BASE COUNT          591 a         1191 c         1156 g          567 t
ORIGIN      
        1 gcatgcaaac gaccgtgccc tcgtcggcac acgcccgcca caacggatcc cagtggtcct
       61 ggtagatgct cggcaggccc aggtcgtacg ggctggacga gaacgtcacc gcgtggcaac
      121 gcttggcagc cacccggcgc acctcggccg cggtggcctc gggatcccac atcatcggca
      181 gcgcaagcgg aatcatacgg cccggatagg ttccggccca ctcgtcgatg tgccagtcgt
      241 tgtaggcgcg caccatcact gccgcctggt cacgattctg tgttcccatg aacaattggc
      301 cgcagaaccg ggggaacgac ggaaagcaca tcgatcccag cacgccgttg gcgttcatgt
      361 ccttgacccg gtcgtgaatg tcgtagcagc ccggtcgaat ttcctcgatg caggtgggct
      421 ccatcccgaa ctcgcttggc gggcgacccg ctaccgcgtt gagcgccgtg ttgaggatct
      481 cctggccctc gtagcgccat gccatcgtcc cgtcgtcgcg ccggacgaag cgcggtgcct
      541 tgtcctggta cttcttcggg acccggccct cgaacatgtg cgccggttcg atgacgtggt
      601 catcgacact gaccaggatc agatcatcga cgttcatgtg tcctcctcat tcaagacagg
      661 cgctcaatcg gcaaggcgcc cagttcaatc catcaggtcg agcatcagtc ggtgaccgag
      721 ttcggactcc tcgatccagg gtgcgttggc ctccagcgcc gccgtccaca cgtcgacggc
      781 acgggcggag tcacggccgg acgcggccga gacgaactcc gaaaacagcg cctgggtgtt
      841 gcggttggtt gtgctcaccc acggcagccc tttcgcatcg gcggtgaccg ccggctccac
      901 accgacacgg acccagtgca agatctcggc gatgacggtc agcgccgggt tcttgacggc
      961 agcgaacgcg agcatctgcg tctcgcgtag cttcgtcacg aaccgggcgg tgtcctcagt
     1021 ggtggccgcg agctcggaat ctgtaacagc cagcgcctta agctgtttgg tgccgatgcg
     1081 ggaggcgacc agacccatga tggcgggttc gacgagctga agggcccggt agaagtcggc
     1141 gagcgtcgtg ccgcgcgcct ccagcacgat gccggcgagc tgcgcggcca ctcgggtgga
     1201 gggtgtgcgg atggtggccc cattccggtc cccggtgcgc aggtcgagca ggaactccat
     1261 ctccaggatc cgcagcgctt ctcgcaatgt gggtcgcgag atcccgaatt cgtcggccaa
     1321 ctctgccagt ggcggcagcc ggtcgccctc gccgagtcgg ccttcggcga tacgggaacg
     1381 gatctcggtg gccacgaccg ctgcacgccg cggcgtcggc tcggcgcccg actgtcgggc
     1441 ctgggcccgc cacagcggcg ccatgtcgat gggctgccgg ctgacgttgc gactgaccag
     1501 catccggctg gtggtgaaaa ggtaatcgct ccaagtcttt tgggccaacg acatgtcgcg
     1561 acggcgggcg gcatccaaaa atgcgctgtg gctgacgatc acccgccggg caatgcgctc
     1621 cgccagcacc gcatcgtcgc taccgatcgc cgagtagacc tggccggcgt agatatcgcg
     1681 gaagacaccg gcgatgacgg cgagggtccg gttacccgaa agttcggtga cggcctggtc
     1741 gaatgcggtc attgccgtga cgaacgacag cggatcggcc tccgcggccc gctcggcgtc
     1801 gtgcagagcg acgagggagt cgagatcctc gtatccagcc cggatggcga cttgttcagc
     1861 ggccgggggc tcgaccaccg accgggcctc ttcgatgtcg gccagggtgg tcctgcgcaa
     1921 ctgcaacaac agcgccacat accggcccac cgcttccaga ccgggccggc ggacgaccgc
     1981 accgccaccg cggccgcgcc taatctccac gagctgctgg gactccagaa tcctcagcgc
     2041 ttcccgcagc gtctcccggg agatccgcag gcgcgctgtg agctcggcct ccgacggcag
     2101 ctgctggtcg gccgcgagct gcccgttgag aatttgacgc cgtaggtcag cggccagcag
     2161 gtgggacacc ttctgagtgt tgcgtcgcgg gatggcgcgc cagacgacgt cgtcggcacg
     2221 gcgctctact ttggattcag gtgactcagt catgatgccg tcaataccat tgcgccgcat
     2281 tgcgggtaca ccccgctggt tacgacggcg actcgcgcgt caggaacctg ccggtcctcg
     2341 cattggcccc taagttggag caccgcctcg tttaagaagc cgatgccgtg caggcgtcct
     2401 tcggcgagct ggcctccggc ggtgtttaga gggaaatcgc cgcccggcgc gatccggctg
     2461 ccgtcgccga cccagtcacc aaactcgccc cgaccgcaga cgcccagcgc ctcgagccag
     2521 gccacggtca ccggagtgaa accgtcgtag acctgggcga catcgacgtc ggccagcgtc
     2581 accgacgacc gctgccagag ccggttcgcg cagtcgacag ttgcgccgta gaggaagtcg
     2641 gagtcaaaga tccagtcggc tccccagcct gtggcgtagg tcatggcgtc gaccagcacg
     2701 gccggctgac gcagatccgc ggcgcgttcg gcggtggtga tgacggtggc ggccgcgccg
     2761 ttgacggggt agtcgcagtc cagcacgcgc agcgggtcgg cgaccatccg cgacgacaga
     2821 tagtcctcaa tcgtcacttc gtcacgcagc accgcgcgcg ggttcagcgc agaccagcgt
     2881 ctggcgttga cggcgatgcg cccgaaatcc tcttcgggac ggccgtattc ggccatccag
     2941 cgttgcttct tcaacgcgag caggacgagg atgccgccca gatagccgta cggggtgagg
     3001 aactggtcgc gcccgcccac tgtctccgga ccgctggcaa cacccccctg gtggccagcg
     3061 gcgcgagtaa ggcacctgaa gacgagcgcc gtctcgcacg cacccgaggc gacggccatg
     3121 acgccggcca gcgcacccgc cagccccgat gggttggagg gcatgatgtc ggcgaaggcg
     3181 gccaaatccg gcaccccgag cagccgagcc atgtcctgcg cgcccgggga ttcgggcccg
     3241 aacttgtact cgaagatccc gtcgatatcg gtggccccca gaccggcatc ggacagcgcg
     3301 accttggccg cggtcagcgc gagattcctc ggatcgggtg caccggtgcg gctcaggtgt
     3361 gaatacccga caccggcgat ggcgacaggg cgcgtcagcg agctcacgtg gccgtctccg
     3421 acagtctgaa ctgcggcagc gtgaccccgc cgcggtcctc gaatacgacc tcgaccggca
     3481 tgccgcatgc cagcactgtc ccggc
//
LOCUS       DASWBF010000370         9680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_957948,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000370 DASWBF010000000
VERSION     DASWBF010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957948"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..1214)
                     /locus_tag="VF874_13765"
     CDS             complement(348..1214)
                     /locus_tag="VF874_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEX9177090.1"
                     /translation="MNQDLVGLAFAAGLVAALNPCGFAMLPAYLALVVRGQDAGRVAA
                     LGRALAATAAMAVGFVAVFGLFGLLTVSAASTLQRYLPYVTAVIGIVLVALGIWLVAG
                     RELAMPSWFGRGARWAPTARLGSMFGYGVSYAIVSLSCTVGPFLAVTGASLRGETVLG
                     GVAVYLAYAAGITLVVGVLAVATAFASSALADRLQRILPAVNRLSGALLVIVGLYVGY
                     YGVYEVRLFEANGNPRDPVVVAAGRLQGALAGWVHRHGGWPWLVGLVVLLTAGLTVAW
                     WDRRAARKGRGA"
     gene            complement(1228..1734)
                     /locus_tag="VF874_13770"
     CDS             complement(1228..1734)
                     /locus_tag="VF874_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEX9177091.1"
                     /translation="MTGRLSRLVTSLAMALAVTALVFGLSASPRAIADDRLNFTGTTL
                     SGAPFNGATLQGKPAVLWFWTPWCPFCNAEAPGISQVAASHPNVTFVGIAARSDVGQM
                     QSFVSKYGLNFTNLNDADGSIWARYEVPWQPAYVFYRADGSSTFVNNPTAAMPEQELA
                     DRVAALTA"
     gene            1809..3104
                     /locus_tag="VF874_13775"
     CDS             1809..3104
                     /locus_tag="VF874_13775"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX9177092.1"
                     /translation="MPTLLWFRRDLRLRDLPPLLDAAGDGADVLACFVLDPRLEGSSG
                     RRRLQFMCDSLRQLRNGLDGRLFVTRGRPEERIPVIANEIGASAVHITEDFTPFGRRR
                     DERVRDALGDIPVHATGSPYLVSPGRVTKDDGNPYKVFSPFLDRWRDVGWRAPARSSA
                     KSARWMDPAGLPGAVEIPDPGVDIDIKAGEQAALRQWARFRDRVLDDYAEDRNRPDKD
                     GTSRMSAHLKFGTIHPRTIVADLDARRKGHAAYLRELAFRDFYASVLYHWPDSGWWNW
                     NRNFDAIDVDTGPAAKKLFEAWKAGTTGFPIVDAGMRQLRETGFMHNRVRMITASFLV
                     KDLHLPWQWGARWFLEQLVDGDMANNQHGWQWCAGCGTDAAPYFRVFNPTTQGEKFDP
                     SGEYIRRWVPELDSGDYPEPIVDHARERKEALRRYGQIS"
     gene            3129..3383
                     /locus_tag="VF874_13780"
     CDS             3129..3383
                     /locus_tag="VF874_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2631 domain-containing protein"
                     /protein_id="HEX9177093.1"
                     /translation="MASTEVERRTGVDVADVPSAEWGWSGDAPRLWRWVGIVIAVFLL
                     AMMRGNHVGWVENWFLIAIAALILGFVARDWYLRRKGLSR"
     gene            complement(3380..4441)
                     /gene="rlmN"
                     /locus_tag="VF874_13785"
     CDS             complement(3380..4441)
                     /gene="rlmN"
                     /locus_tag="VF874_13785"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9177094.1"
                     /translation="MSNAGPPRHLADLDEAGRVDAVAAVGLPAFRAKQLAHQYYGRLI
                     ADPRQMTDLPAAVRETVADAIFPPLLTPAREIECDDGLTRKTLWHTPDGTAFESVLMR
                     YPQRTTVCISSQAGCGMACPFCATGQAGLTRNLSTAEIVEQVRSAAAYMRDIGSRLSN
                     VVFMGMGEPLANYNRVLAAVRRIVDPPPNGFGISQRSVTVSTVGLVPAIRKLADEGLG
                     VTLALSLHAPDDELRDTLVPVNNRWNVAEALDAARYYADATGRRVSVEYALIRDINDQ
                     PWRADLLGKRLHAALGPLVHVNLIPLNPTPGSRWDASPKPAEREFVRRVRAHGVSCTV
                     RDTRGREIAAACGQLAAES"
     gene            complement(4443..5312)
                     /locus_tag="VF874_13790"
     CDS             complement(4443..5312)
                     /locus_tag="VF874_13790"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX9177095.1"
                     /translation="MAETDTGAPGQGDAPKTSRTGRNLPAAIGVGVVLGAMAIGVLLF
                     VPHAWFGVLAVAIPIATHEVTRRLREAGYAIPIVALLIGGQAILWLAWPLGPAGALMG
                     YGAAVVVCMVWRLVGQGLNEQPVNYLRDISATVLLATWIPLFASFSALLIRQDHGGAR
                     VFALIATVVFADIGGYTAGVLFGKHLMVPAISPKKSWEGLGGSLLFGIAAAVLSVTIL
                     LDKPWWVGVPLGLLLVITGVLGDLVESQVKRDLGIKDMGTLLPGHGGLMDRIDAMLPS
                     AVAGWIVLTLLAT"
     gene            complement(5338..5895)
                     /gene="frr"
                     /locus_tag="VF874_13795"
     CDS             complement(5338..5895)
                     /gene="frr"
                     /locus_tag="VF874_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX9177096.1"
                     /translation="MIDETLFEAEEKMEKAVAVARDDLASIRTGRANPGMFARISIDY
                     YGAMTPITQLSSINVPEARLVVIKPYEQSQLGAIEKAIRNSDLGVNPTNDGNVIRVAI
                     PQLTEERRRDLVKQAKSKGEDARVSVRNIRRKAMEELHRIRKGGEAGEDEVKHAEKDL
                     EKTTHQYVEQIDDLVKHKEGELLEV"
     gene            complement(5907..6683)
                     /gene="pyrH"
                     /locus_tag="VF874_13800"
     CDS             complement(5907..6683)
                     /gene="pyrH"
                     /locus_tag="VF874_13800"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX9177097.1"
                     /translation="MAEFNGASSPPPEAAIPPPPPPERPPYRRVLLKLGGEMFGGGQV
                     GLDPDVVHLVARQIAEVVRSGVQVAVVIGGGNFFRGAQLQQRGMERTRSDYMGMLGTV
                     MNSLALQDFLEKEGIATRVQTAIAMGQVAEPYIPLRARRHLEKGRVVIFGAGMGLPYF
                     STDTTAAQRALEIGADVVLMAKAVDGVFTDDPRYNPNAELLTEITHREVIDRGLRVAD
                     TTAFSLCMDNGMPILVFNLLIDGNIARAVAGEKIGTLVTT"
     gene            7089..7958
                     /locus_tag="VF874_13805"
                     /pseudo
     CDS             7089..7958
                     /locus_tag="VF874_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
     gene            8154..8876
                     /locus_tag="VF874_13810"
     CDS             8154..8876
                     /locus_tag="VF874_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177098.1"
                     /translation="MASAPRPPSASARRLRWILPAAALAATLLVVGGFVIWQRTRPST
                     TSATSATTTSISATTASAIPTVDFRSWQPFGGIDAQVSGDGRSVVLDTHDTTENWKAV
                     WSGLIAPGPPQCSTRITGQARDINHTLAAAGGFGIGLTTIQTDSSGQEAAFGSAVQYD
                     FGLGGYFAVRYPTAESYGLVAAPLDHEWHNFDVTIDEQGWITAQIDGKTAVHAKGAAV
                     CGLPTLRVWAGSAEFREFSVQP"
     gene            complement(8897..>9680)
                     /locus_tag="VF874_13815"
     CDS             complement(8897..>9680)
                     /locus_tag="VF874_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9177099.1"
                     /translation="GVVTRSVRDTAAFYRVAEQFWRSRKLPPIGDITRPGTERLTIAV
                     VTRSIFRESSPEVRELTLKTAALLEELGHRVHHLDSPSVPAYFADDFMLYYGFLGFAL
                     VRNGRRMFGASFDRSKLDNLTLGFERHAARNMHRLPLAIARLSVMRRRTARLFRTYDA
                     VLTPTVAEPPPAIGHFDPGADYQQIIDRLVDWVAFTPLQNATGDPAISLPLAESAAGL
                     PVGMMFTAAHGQERRLLELAYELEEARPWRRIQDALPVSPEV"
BASE COUNT         1663 a         3290 c         3140 g         1587 t
ORIGIN      
        1 ggttgatcac cggaggaggg ggactgggcg gcggcggcaa cgacgcgccc aagctcggcg
       61 gtatcacgat gcgtcccacc cagtccggca gcgagaacgg gttgaaggcc gtcggcgcca
      121 gttggacatc cgccgcaagc cgaatgctgg gcgcggccat aggctcaggc gcgggcggct
      181 gtaccgacgg cgtgaacgca atcgcactgg ctgtgatgac tgcggcaccg gctgtcgctg
      241 ccttctttgc aaacgccttc atgttcggat cccctcgatc gccttatcgc gtgtgaccct
      301 atccggtgag cgggtctgtc cgcagcaaaa cttgcagacg aaagaattta cgcgccgcgc
      361 cctttgcgag cggcgcggcg gtcccaccac gccaccgtca acccggccgt cagcaacacg
      421 acgagcccca ccagccaggg ccacccgccg tgccgatgca cccatccggc gagcgcgccc
      481 tgcagccgcc cggcggcaac caccacgggg tcacgggggt ttccgttggc ctcgaacagc
      541 cgcacctcgt acacgccgta gtacccgacg tagagcccga cgatcaccag cagcgcgcca
      601 ctgagccggt tgacagcggg caagatccgc tgcagacggt ctgccagcgc cgaactcgcg
      661 aacgccgtcg cgacggccag cacgccgacc accaatgtga ttccggcggc gtaagcgaga
      721 tacacggcca caccgccgag aaccgtctca ccgcgcagac tcgccccggt gaccgccagg
      781 aatgggccga ccgtgcacga cagcgacacg atcgcgtaac tcactccgta cccgaacatc
      841 gagcccagcc gggcagtcgg cgcccaccgc gcgccgcggc caaaccagct gggcatcgcc
      901 agctcgcgac ccgccacaag ccaaatacca agtgccacaa gcactatgcc gatcaccgcg
      961 gtgacatacg gcagatagcg ttgcaacgtc gaggcggccg acaccgtcag cagcccgaac
     1021 agcccgaaca ccgcgacgaa gcccaccgcc atcgcggccg tcgccgccag cgcgcggccc
     1081 agcgctgcaa cacggccagc gtcttggccg cgaaccacca aggcgaggta cgcgggcagc
     1141 atcgcgaatc cgcacgggtt cagcgccgcc accagaccag cagcgaaggc caagcccacc
     1201 aggtcttggt tcacttcggc cggcggtcta cgccgtgagc gccgccactc ggtcggcaag
     1261 ctcctgctcg ggcatcgcgg cggtcggatt gttcacgaaa gtcgatgagc cgtccgcccg
     1321 gtagaacacg taggccggct gccacggcac ctcgtagcgc gcccagatgg atccgtcggc
     1381 gtcgttgagg ttcgtgaagt tcagaccgta tttggacacg aagctctgca tctgcccaac
     1441 gtcggaccgt gcggcgattc cgacgaacgt cacgttggga tgcgacgcgg ccacttggct
     1501 aatcccaggt gcttcggcat tgcagaacgg gcaccacggc gtccagaacc acagcacggc
     1561 cggcttgcct tgcagggtgg ccccgttgaa cggcgcgccg gacagcgtgg ttccggtgaa
     1621 gttcagccgg tcatcggcaa tcgctctcgg tgaagcgctc aacccgaaca ccagggcggt
     1681 cacggccaag gccatcgcga gcgaggtcac taatctcgag agccgaccgg tcatgacatc
     1741 ccctccagtc aaaaaagtgc cctaggtcac agtagtgacc cgaatacccg gcgtgaaagg
     1801 ttcattcggt gccgacgctg ttgtggtttc gccgcgatct gcgactgcgg gacctgccgc
     1861 ccctgttgga cgccgccggc gacggggccg acgtgctggc gtgtttcgtg ctcgaccctc
     1921 ggctggaggg gtcgtcggga cggcggcggc tgcaattcat gtgcgactcg ctgcggcagc
     1981 tgcgtaacgg cctcgacggc cggttgttcg tcacccgtgg acggccagaa gagcgaattc
     2041 cggtcattgc caacgaaatt ggcgcctcgg cggtgcacat cacggaggac ttcacgccgt
     2101 tcggccgccg ccgcgacgaa cgggtgcgtg acgcgctcgg ggacataccg gtgcacgcga
     2161 cgggctcgcc gtacctggtg tctccgggcc gcgttaccaa ggacgacggc aacccgtaca
     2221 aggtgttttc gcccttcttg gaccgatggc gcgacgtcgg ctggcgcgcg ccggcccgtt
     2281 cgagcgccaa gtcggcccgc tggatggacc ccgcaggcct gccgggcgcg gtggagattc
     2341 ccgatcccgg cgtcgacatc gacatcaagg ccggtgagca agcggctctg cgacagtggg
     2401 cgcgctttcg cgatcgcgtt ctcgacgact acgccgagga ccgcaaccga ccggacaagg
     2461 acggcaccag ccgcatgtcg gcgcacttga agttcggcac catccatccg cggaccatcg
     2521 tcgcggatct ggacgctcgc cgcaaaggtc acgcagctta cttgcgggag ttggcttttc
     2581 gcgatttcta cgcgtcggtg ctctatcact ggccggacag cgggtggtgg aactggaacc
     2641 gcaatttcga cgcgatcgac gtcgacaccg gcccagccgc caagaagctg ttcgaggcgt
     2701 ggaaggccgg caccacaggt tttccgatcg tcgacgccgg catgcggcag ttgcgcgaaa
     2761 ccggcttcat gcacaaccgg gttcggatga tcaccgcctc gtttctcgtc aaggacttgc
     2821 acctgccgtg gcagtggggc gcacgctggt tcctggagca gcttgtcgac ggcgacatgg
     2881 ccaacaacca gcacggctgg cagtggtgcg ccggctgcgg caccgacgcc gcgccgtact
     2941 ttcgcgtgtt caacccgacc acgcagggcg aaaagttcga tccctcaggc gagtacatcc
     3001 gccggtgggt gcccgagctc gacagcggcg actaccccga gccgatcgtc gaccacgccc
     3061 gcgaacgcaa ggaagcgctg cgccgctacg gtcagatcag ctgaggctcg tatgccagaa
     3121 tgtcgggcat ggccagcacc gaggttgaac gacgcaccgg agtcgacgtc gcggacgtac
     3181 cgtccgccga atgggggtgg agcggcgacg caccccggct gtggcgttgg gtcggcatcg
     3241 tcatcgccgt attcctgttg gcgatgatgc gcggcaatca cgtgggctgg gtggagaact
     3301 ggtttctcat cgcgatcgcg gcgctgattc tggggttcgt cgcccgcgac tggtacctgc
     3361 gccgtaaggg cctcagccgc tagctttcgg ccgccaactg tccgcacgcg gcggcgatct
     3421 cgcggcctcg ggtgtcgcgc acggtgcacg acactccgtg tgcgcggacg cgtcggacga
     3481 actcccgctc ggctggtttg gggctggcgt cccagcggct gcccggcgtc gggttcagcg
     3541 gaatcaggtt gacgtgcacc agcgggccga gcgcggcgtg aagccgcttg ccgagcagat
     3601 ccgcccgcca cggctggtcg ttgatgtcgc ggatcagcgc gtactccacc gacacccgcc
     3661 ggcccgtcgc gtcggcgtaa tagcgcgcgg cgtccagcgc ctcggcgacg ttccagcggt
     3721 tgttcaccgg caccagggtg tcgcgcaact cgtcgtcggg tgcgtgcagc gacaacgcca
     3781 gcgtcacacc cagaccctcg tcggcgagct tgcggatcgc gggcaccagc cccaccgtcg
     3841 acaccgtcac cgagcgctgc gagatcccga aaccgttcgg cggcggatcg acgatgcggc
     3901 gcaccgcggc cagcacgcgg ttgtagttgg ccagcggctc gcccatcccc atgaagacga
     3961 cgttggaaag ccgtgaaccg atgtctcgca tgtatgccgc cgcggagcgc acctgctcca
     4021 cgatttcggc cgtcgacagg ttgcgggtca gcccggcctg cccggtcgca cagaacggac
     4081 acgccatgcc gcaccccgcc tgcgaggaga tgcacacggt ggtgcgctgc ggataccgca
     4141 tcagcacgga ctcgaaagca gtgccatcag gcgtgtgcca caacgtcttt cgggtcaggc
     4201 cgtcgtcgca ttcgatctcg cgcgccgggg tgagcaacgg cgggaagatc gcgtcggcca
     4261 ccgtttcccg caccgcggca ggcaggtcgg tcatctgccg cgggtcggcg atcagccgtc
     4321 cgtagtactg gtgcgccaat tgcttggccc ggaacgcggg cagcccgacc gcggcgacgg
     4381 cgtcgacgcg gcccgcttcg tcgaggtcgg caaggtgccg cggcggacca gcgttggaca
     4441 tgtcaggtcg caagcagggt cagcacgatc cagccggcga cggccgaagg cagcatcgcg
     4501 tcgatgcggt ccatcaggcc cccgtggcct ggtagcagcg tgcccatgtc cttgataccg
     4561 aggtcacgct tgacctgaga ctcgaccagg tcgccgagca cgccggtgat caccaagagc
     4621 aggcccagcg gcacgccaac ccaccacggc ttgtcgagca ggatcgtcac cgacagcaca
     4681 gcggcagcga tcccgaacag gagcgacccg ccgagtccct cccacgattt cttcgggctg
     4741 atcgccggca ccatcaaatg cttgccgaac agcacgcccg cggtgtagcc gccgatgtcg
     4801 gcgaagacga cggtcgcgat gagcgcgaaa actcttgcgc caccgtgatc ttggcggatc
     4861 aacagcgcac tgaagctggc gaacaacggg atccacgtcg ccaacaacac cgtggccgag
     4921 atgtcccgga ggtaattgac cggctgctcg ttgaggcctt ggccgaccag ccgccacacc
     4981 atgcagacga cgacggcggc gccgtagccc atcagcgccc cagcgggccc gaggggccac
     5041 gccagccaca gaatggcctg gccaccgatc agcaaggcca cgatcggaat cgcgtagccc
     5101 gcctctcgta gccggcgcgt cacctcatgg gtggcgatcg gaatcgcgac cgcgagcaca
     5161 ccgaaccatg cgtgcgggac gaacagcagg actccgatcg ccatcgcgcc cagcacgacg
     5221 ccgacgccga tcgcagcggg cagattgcgg ccagtgcgtg aggtcttggg tgcgtcgccc
     5281 tggccggggg cgccggtgtc ggtttctgcc acggaattcg gtcgctgctg ggtccgtcta
     5341 gacctcaagc agctcgccct ctttgtgctt cacgagatcg tcgatctgct cgacgtactg
     5401 gtgcgtggtc ttttcgaggt ccttctcggc gtgcttgacc tcgtcctcgc cggcctcgcc
     5461 gcccttgcgg atgcggtgca gctcttccat cgccttgcga cggatgttgc gcaccgacac
     5521 cctggcgtcc tcgcccttcg acttggcctg cttgaccagg tcgcggcgcc gttcctcggt
     5581 gagctgtggg atcgccaccc ggatcacgtt gccgtcattg gtcgggttga cgccgaggtc
     5641 ggagttgcgg atcgccttct cgatcgcgcc cagctgcgac tgctcatacg gcttgatcac
     5701 gacgagccgc gcctccggca cgttgatgct ggagagctgc gtgatcggag tcatcgcgcc
     5761 gtagtagtcg atggagatgc gggcgaacat gccggggttg gcgcgaccgg tgcgaatcga
     5821 cgccaggtcg tcgcgggcca cggcaacggc cttttccatc ttctcttcgg cctcgaagag
     5881 ggtctcgtcg atcacggcat tctccctcag gtggtgacca gtgttccgat cttctcacct
     5941 gcgacggccc gggcgatatt gccgtcgatg agcaggttga agaccaggat cggcatgccg
     6001 ttgtccatgc agaggctgaa ggcggtggtg tcggccaccc gcagcccgcg gtcgatgacc
     6061 tcccggtggg taatctcggt gagcaactcg gcgttcgggt tgtagcgcgg gtcgtcggtg
     6121 aagacgccgt cgacggcctt ggccatcaga accacgtcgg cgccgatctc cagtgcgcgc
     6181 tgcgccgcgg tggtgtcggt ggagaagtac ggcaggccca tgccggcgcc gaagatcacc
     6241 acccggccct tctcgaggtg gcgacgcgcg cgcagcggaa tgtacggctc ggccacctgt
     6301 cccatcgcga tggccgtctg aacccgggtg gcgatgccct ctttctccag gaagtcttgc
     6361 agcgcaaggc tattcatgac ggtgccgagc atgcccatgt agtcggaacg cgtgcgttcc
     6421 atgccgcgct gctgaagctg cgctccgcgg aagaagttgc cgccgccgat cacgacggcc
     6481 acctggacgc cgctgcggac gacctcggcg atctggcggg ccaccaggtg gacgacgtcg
     6541 gggtcgagcc cgacctgccc gccgccgaac atctcaccgc cgagcttgag cagcacccgc
     6601 cggtaaggcg gccgctccgg cggtggcggt ggtggaattg ccgcctccgg cggcggtgat
     6661 gacgcgccgt tgaactccgc catcagcctc cttcatccgc ctcgcatgag agagccatcg
     6721 gaggcagtcg gcccacgacg gcagtctcat cctgcctcat caacgccgcc cgatggaccg
     6781 gggtcggacg attcgtccct aagtcggctc gtctacttcg tcgtccgcga gcattcgttc
     6841 tgggtggaag tggtgttggt tcgccgttgt ccgcggtcca cgtccctgag ccgcaatatg
     6901 catcggagac accccgttgc gtggttagcc gaatgggcag tgcggcgttc acgagggacg
     6961 gatactaagg cgcgctgtgt cggcggtggc cagaccccgg aagtcatacg gcgaagacgg
     7021 cgggagacgg acttgtgttg cggtcacgag ttgaagcgtt ccttgtggaa tcggcgtgag
     7081 acgcgtccat gaccactcaa tcacccggtc cgcctgtcga tgatccgcaa cgtggcggcg
     7141 ggactccgtt cggccagtat cgactgatca acttgctggg ccgcggcggc atgggcgagg
     7201 tatggcgcgc ctatgacact gtcaccgagc gcgtcgtcgc gctgaaactc cttccgccgc
     7261 actttgcaga agacagcgtg ttcaaacagc gcttccgccg ggaagcccac gccgctgcgc
     7321 agctaaacga gccgcatgtg gtgcctattc acacctacgg ggagatcaac ggacgcttgt
     7381 tcgtggatat gcggctgatc gagggacgcg atcttgacgt tgtgctggcc gaggggccgc
     7441 tgtccccaga gcgagcggtg ctgatcatcg agcaggtggc cagggcagtc catgccgcgc
     7501 acaaggtcgg gttggtgcat cgggatatca agccgtccaa cattttgctc gacgaccacg
     7561 actttgcgta cttgatcgat tttgggatcg cgcgcgccgc aggcgacacc ggcttgacgg
     7621 cgaccggcgg gctgatcggc accctgcgct acatggcgcc cgagcggttc ggcagcgccg
     7681 gggccgatgc ccgctccgac atttacgcgc tggcctgtgt gctatatgag tgcctgaccg
     7741 gcagccgccc atttcccggt gagagcctgg aacagcaact ggccgggcat ctggcggtcg
     7801 cgccgccgcg accgtcgcac aaccacttcg gcgtgccggc cgagttagat gccgtgattg
     7861 ccaccggcat ggccaaagac cccggcgcgc gctatcccac ctctgtcgag ttggctcgag
     7921 cggcacgcga cgcgatcact acgccgcttt ctcgcccggc accgaccatt ccgccgcagc
     7981 caccgaccat gcccgcacca atgcgattga ccacgcctcg ggatgggccg ccgacgaatc
     8041 gtccccagcc gcaacgcccg ccaccgtcac gtccgtatcc gggccctgtc tggcagccac
     8101 cgccgtctgc tcaaccatcc atcgagcagc cagccataca gccagccata taggtggcgt
     8161 cggcacccag gccgccgtcc gcctcggccc gtcgattgcg ttggatcctg cctgcggccg
     8221 ccctggccgc cacgttgttg gttgttgggg gttttgtcat atggcaacgc actcgcccgt
     8281 cgaccacctc agcgacatcg gcgaccacca catctatatc ggcgaccacc gcatctgcca
     8341 tccctaccgt cgacttccgc agctggcaac cgttcggcgg catcgacgcg caagtcagcg
     8401 gagacggtcg gtcggttgtt ctcgacacgc acgacaccac cgagaactgg aaggcagtat
     8461 ggtcaggcct catcgcgccc ggaccgccac aatgcagtac ccgcataaca ggacaggccc
     8521 gcgacatcaa ccacactctc gctgccgcag gaggattcgg tatcggcctg acgacaatac
     8581 agacggactc ttccggccaa gaagccgctt tcggctcggc ggtgcagtac gacttcggct
     8641 tggggggcta tttcgccgtg cgttacccaa ccgctgagtc atacggcctc gtcgcagcgc
     8701 cacttgacca tgaatggcac aactttgatg tcacgatcga cgagcagggc tggattacag
     8761 ctcagatcga cggaaagaca gccgtacatg ccaagggcgc cgcggtatgt gggctgccga
     8821 cgctccgcgt ttgggctggc tccgcagaat tccgggagtt ttccgtacag ccgtgagtgg
     8881 cgggcccgcg ccgatttcac acttcggggc tgaccggaag tgcgtcctga atgcgccgcc
     8941 atggccgggc ctcttccagc tcgtacgcta actcgagaag gcggcgttcc tgaccgtgcg
     9001 cggccgtgaa catcatcccg accggcagcc ctgccgccga ttccgcaagc ggcagcgaga
     9061 tcgccggatc cccggtggcg ttctgcagcg gcgtgaacgc gacccagtcg acgagccggt
     9121 cgatgatctg ctgatagtcg gcgccgggat cgaagtgccc gatcgccggc ggtggctcgg
     9181 ccaccgtcgg cgtaagcacg gcgtcgtagg ttcggaatag ccgagccgtt cgccgtcgca
     9241 tcaccgaaag ccgcgcgatc gccagcggca gccggtgcat gttgcgggcg gcgtgccgct
     9301 cgaagcccag cgtcaggttg tccagcttgc tccggtcgaa acttgccccg aacatccgcc
     9361 gcccgttgcg caccaacgca aaaccgagaa acccgtagta cagcatgaag tcgtccgcga
     9421 aataggctgg cacggacgga ctgtcgagat gatggacccg gtgtccaagt tcctccagca
     9481 gcgcggctgt cttcaacgtc aactcgcgca cttcaggcga ggactcacgg aatatcgacc
     9541 gggtgaccac cgcaatggtc agcctttcgg tacccggacg agtgatgtca ccgatcggcg
     9601 gcagcttgcg gctgcgccaa aactgctcgg ccacacggta aaatgccgcg gtgtcccgca
     9661 ctgagcgtgt caccacgccg
//
LOCUS       DASWBF010000371         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_958170,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000371 DASWBF010000000
VERSION     DASWBF010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958170"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VF874_13820"
     CDS             complement(<1..107)
                     /locus_tag="VF874_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9177100.1"
                     /translation="MAQSTRRTGILVGVDGSAESDAAIRWATTEAVMRRA"
     gene            complement(109..507)
                     /locus_tag="VF874_13825"
     CDS             complement(109..507)
                     /locus_tag="VF874_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX9177101.1"
                     /translation="MTWARDAVRIFNKHALNPVMMRMAGRKHWYAGVIRHTGRRTGKE
                     YATPVVADRVAGGFIVPLPYGTGVDWLRDVLAADRATIQAGGQTCQVVHPVVIDAEAA
                     ASQLSPRRRRAFQRFGIDRYLKIEVADSGE"
     gene            complement(504..767)
                     /locus_tag="VF874_13830"
     CDS             complement(504..767)
                     /locus_tag="VF874_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEX9177102.1"
                     /translation="MRARVGDWLVIKGTKVDKPDQRGLIEEVHSADGSPPYVVRWLAT
                     DHVAIVFPGPDALIVTPEEQEAADERARSRAASVQSSIRHESR"
     gene            complement(783..1766)
                     /locus_tag="VF874_13835"
     CDS             complement(783..1766)
                     /locus_tag="VF874_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HEX9177103.1"
                     /translation="MTRPTVDTEVITKAILLACRAPSLHNSQAWLWVVDDGVVELFSD
                     HTRTVRSTDSSGRQVIISCGAALGHFRVAMSAAGWDTNVDRFPNPNELDHLVTVDFSP
                     AHFVTPAQRDRADAILVRRTDRLPFGAPTDWDSFESVLRTTFDHTVARLDVLPDDARP
                     TLAEATRLTESLRRYDQYYHDELRWWTPSFSIGDGLPRSALVSEEENARVEVNRQFPV
                     GGHGERRPDLQADRAKILLISTPENTRADALNCGEVLSTVLLEATMAGLATCPLTAVT
                     ELAESREVIENLVGGGGFPQVLIRVGQAPALEERPAPTPRRPLSEVMKIRH"
     gene            1895..3388
                     /locus_tag="VF874_13840"
     CDS             1895..3388
                     /locus_tag="VF874_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9177104.1"
                     /translation="MSTGAAAPASAGAATPYAEIRETHTGVVVLVGDRAYKGKKPITT
                     DFLDHSTAESRERSCQREVELNRRLAPDTYLGVAHMSDPSGGPAEPVVVMRRYPEACR
                     LATRVRNGEPVTSALERVADELARFHERADRGRAIDEQGKVRAVRALWQENIAELKRF
                     VGSMLPADKVRDLEDLATQFIAGRSVLFTARVEDRCIVDGHGDLLADDIFCLSDGEVE
                     ILDCLEFDDALRHVDVIDDAAFLAMDLEFLDGRELADQFLESYGRLSGDTAPSSLKHF
                     YIAYRAVVRAKVDCIRVNQGHPDAAQDALRHVDMAVDHLQAGAVRLVLVGGGPGTGKT
                     TVAHGLADRVGAQVVSTDEVRRELQQSGTVTGTAGVLDRGLYSPENVGEVYHAVLRHA
                     HLLLAGGRSVILDGTWRDPRHRAAARQLAADTHSALSEILCVATAEAAASRVAGRPLG
                     GASDATPGIAEALKDAGQDWRDAFRLDTDQPLADTVRDAETLWRDMV"
     gene            3402..>4711
                     /locus_tag="VF874_13845"
     CDS             3402..>4711
                     /locus_tag="VF874_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9177105.1"
                     /translation="MTAAITERGELVDTPLPPARVASADVGEVLAWLATSADGLSSAE
                     APARRARFGPNSVRTHRVSAIAVLGRQLRNAVLWLLAGTAAVSFFLGDSTEALIIAVI
                     LAASIGLGFVNEYRAERMAAALHSRVRHTAAVRRDGHFTKLDVNELVPGDVIRLTLGE
                     LVPADVRLIEATGLECNESILSGESIAREKSPSPVASAEGLTDLADLAFMGTIVSAGE
                     GTAVVYASGVNAQFGRLAAGLGQRRPETDFQTGLRQFSYLLLQVAVALTVLILVTNLL
                     LRRPIIESVLFSLAIAVGITPQLLPAVVSTSLATGSRRLARYKVLVKRLVCIEDLGDV
                     DVLMTDKTGTLTEGRITLIDAVDADGAHDDSVLRIGLLVTDVDPESGGASADPLDAGL
                     WESPASQRAAAGTTRRLAVRPFDHIRRSMSVLVDDGDRRLLIVKV"
BASE COUNT          771 a         1596 c         1569 g          775 t
ORIGIN      
        1 gcccggcgca tcaccgcctc ggtggtggcc cagcggatcg cggcgtcgga ctccgcggag
       61 ccatcgacgc caaccaaaat gccggtcctg cgtgtggatt gggccatctc actctcctga
      121 atccgcgact tcgatcttca aataacggtc gatcccgaat cgttgaaacg cgcgtcgacg
      181 cctgggggac aactggctgg cggccgcctc cgcgtcgatg acgaccgggt ggacaacctg
      241 gcaggtctgg ccacccgcct ggatggttgc acggtcggcg gccagcacgt cgcgaagcca
      301 gtcgacgccg gttccgtacg gcagcggcac gatgaaccca ccggccaccc ggtcggcgac
      361 caccggcgtg gcgtattcct taccggtgcg acggccggta tgccgaatca caccggcgta
      421 ccagtgtttt cgccccgcca tgcgcatcat gaccgggttg agcgcgtgct tgttgaaaat
      481 gcgcaccgca tcgcgagccc aggtcatcgg ctctcatgcc tgatcgatga ctgcaccgac
      541 gcggcgcggg accgggcccg ctcgtcggcc gcctcctgct cctccggtgt gacgatcagt
      601 gcgtcgggac cgggaaagac gatggccacg tggtcggtgg cgagccaccg cacaacgtag
      661 ggtggcgagc cgtcggcgga atgcacctct tcgatgagtc cacgctgatc cggcttgtcc
      721 accttggtcc ccttgatcac cagccagtcg ccgactctcg ccctcatatg tacctcccaa
      781 actcagtggc ggatcttcat gacttcgctc aacgggcgcc gcggcgtggg cgctggccgt
      841 tcttcgagag ccggtgcctg cccgacgcga atcaacacct gcgggaaacc gccgccgccg
      901 acgaggtttt cgatgacttc tcggctttcc gccaactcgg tgacggctgt cagcgggcac
      961 gtcgccagac cggccatcgt ggcttccagc agcaccgtgg agagcacctc gccgcagttg
     1021 agcgcgtcgg cgcgggtgtt ctccggcgtc gagatgagca ggatcttcgc ccggtccgcc
     1081 tgtaaatccg ggcgtcgttc accgtgcccg ccgaccggga actgacggtt cacttcgacg
     1141 cgggcattct cctcctcgga aaccaatgcg ctgcgtggca agccgtcgcc aatgctgaaa
     1201 gatggtgtcc accaacgaag ttcgtcgtgg tagtactgat cgtagcggcg cagggactcg
     1261 gtgagacggg ttgcctcggc cagggtcggt cgggcgtcat cgggcaacac gtctagcctg
     1321 gcaaccgtgt ggtcgaacgt cgtgcgcaac accgattcga acgagtccca atcggtgggc
     1381 gcgccgaaag gcagtcgatc cgtgcggcga accagtatcg cgtcggcgcg gtcacgctgg
     1441 gcgggcgtga cgaaatgagc gggcgagaag tcgaccgtta cgagatggtc gagctcgttg
     1501 ggattgggga agcggtcgac gttggtgtcc cagccggccg cggacatcgc gacgcggaag
     1561 tgtccgagcg cggcgccgca actgatgatc acctggcgcc cggagctgtc ggtggaacgc
     1621 acggtgcggg tgtgatcgct gaacagctcg accacgccat cgtcgaccac ccagagccac
     1681 gcctggctgt tgtgcagcga cggagccctg cacgcgagca agatcgcctt cgtgatcacc
     1741 tcggtatcca ccgtgggtcg agtcatcacg gcctcccatc cacttccgag cctaggaaca
     1801 actgaaccgt gtaccagagt ccttggtccc cagcaccgcc accactatgc ccgctgcccg
     1861 atgtcgtccg agaatatgac tcatggggca ggccatgagc accggcgcgg cggcgcccgc
     1921 gtcggcgggc gcagctaccc cctatgcgga gatccgcgaa acccacaccg gggtggtggt
     1981 actcgtcggc gaccgggcat acaaaggcaa gaagccgatc accaccgact ttctcgacca
     2041 ttccactgcc gagagccgcg agcggtcgtg ccagcgcgag gtcgagctca accgccggct
     2101 cgcccccgac acgtatctgg gcgtcgcaca catgagcgac ccatccggcg gccccgccga
     2161 gccggtcgtg gtgatgcgcc gctaccccga ggcctgccgg ctggccacca gggtgcgcaa
     2221 cggcgagccg gtcacctctg cgctggagcg agtcgccgat gagctcgcgc ggttccacga
     2281 gcgagccgat cgtggtcgcg ccatcgacga gcaaggcaag gtccgtgccg tcagggcact
     2341 gtggcaggag aacatcgccg agctgaaacg gttcgtcggc agcatgctgc ccgccgacaa
     2401 ggttcgtgac ctcgaagacc tggccacgca gttcatcgca gggcggtcgg ttcttttcac
     2461 cgcgcgcgtc gaggatcgat gcatcgtcga cggccatggc gacctgctcg cagacgacat
     2521 cttctgcctg tctgacggcg aggtggagat cctggactgc ctcgagttcg acgatgccct
     2581 gcgccatgtg gacgtcatcg acgacgccgc cttcctggca atggatttgg agttcctcga
     2641 tggaagggag ctggccgacc agttcttgga gagttacggc cggctcagcg gcgacacggc
     2701 gccgtcgtcg ctcaagcact tctacatcgc gtaccgcgcc gtggtccgcg cgaaggtcga
     2761 ctgcattcgc gtgaaccagg gccaccccga cgccgcgcag gacgcgttgc gccacgtcga
     2821 catggcggta gatcacctgc aggccggcgc cgtgcgactg gtgcttgtcg ggggcggccc
     2881 gggcaccggc aagaccaccg tcgcacacgg gctggctgac cgagtcggcg cgcaggtggt
     2941 ctccaccgac gaggtgcgac gcgaactgca gcagtcgggc accgtgacgg ggacggccgg
     3001 ggtgctcgat cgcggcctgt actcgccgga gaacgtcggc gaggtctacc acgcggtgct
     3061 tcgccacgcg cacctgctgc tcgccggcgg ccggtcggtg atcctcgacg gcacctggcg
     3121 ggatccacga caccgcgcgg cggcgcgaca gcttgcggcg gacacacatt cggcgctgtc
     3181 ggaaatcctc tgcgtggcaa cggccgaggc ggcggcatcg agagtcgccg gcaggcccct
     3241 gggcggtgct tccgacgcca cccccggcat cgcggaagcg ctgaaggacg ccggccagga
     3301 ttggcgcgac gcattccgcc tcgacaccga ccagccattg gcagacaccg ttcgcgacgc
     3361 cgagacgctg tggcgagaca tggtctgagc tgccaaccga catgaccgcg gccattaccg
     3421 agcgaggcga actcgtcgac acccctctgc cgcccgcgcg cgtcgcgtcg gccgacgtcg
     3481 gcgaggtact ggcgtggctg gccacgtccg cagacgggct gtccagcgcg gaggcgccgg
     3541 cccggcgcgc ccggttcggg ccgaattcgg ttcgcacaca ccgggtcagc gcgatcgccg
     3601 tactgggccg ccagcttcgc aacgcggtgc tgtggttgct cgcgggcact gccgcggtgt
     3661 cgttcttcct cggcgacagc accgaggcgc tgatcatcgc cgtcattttg gcggccagca
     3721 tcgggctcgg cttcgtcaac gaataccgcg cggaacgcat ggcagccgca cttcattcgc
     3781 gggtgcgcca caccgctgcg gtgcgccgcg acggacattt caccaaactc gacgtcaacg
     3841 agctggtgcc cggcgacgtg attcggttga cgctcggtga acttgtgccg gccgacgtcc
     3901 gactgatcga ggccacggga ctggaatgca acgagagcat cctgtcgggt gaatcgatcg
     3961 ccagggagaa gtcaccttcc ccggtcgctt ctgcagaagg cctcaccgac ctggctgatc
     4021 tggccttcat gggcacgatc gtcagcgccg gtgaaggcac cgcggtggtc tacgcgtcag
     4081 gcgtcaatgc ccaattcggg cggctcgcag caggattggg gcagcggcgg cccgagaccg
     4141 acttccagac ggggctgcgg cagttctcct atctgctgct tcaagtcgcc gtcgcgctga
     4201 ccgtgctgat tctcgtcaca aatctccttc tacgccggcc gatcatcgag tcggtgttgt
     4261 tctcgctggc catcgccgtc ggtatcaccc cccaactgct tcccgcggtg gtcagcacca
     4321 gcctcgcgac cgggtcgcgg cggctcgccc gatacaaggt gctggtgaag cggctggtct
     4381 gcatcgaaga cctcggcgac gtcgacgtgc tgatgaccga caagaccgga acgctgacag
     4441 agggccgcat cacgttgatc gacgcggtcg acgccgacgg ggcccacgac gactcggtgc
     4501 tgcgcatcgg gctgcttgtg actgacgttg atcccgagag cggcggcgcc agcgccgacc
     4561 cgctcgacgc ggggctgtgg gaatcgccgg catcacaacg cgctgccgcc ggcacgacgc
     4621 gccgactggc ggtccggccg ttcgaccaca tccggcgatc catgtccgtc ctcgtcgatg
     4681 acggtgaccg tcggttgctg atcgtcaagg t
//
LOCUS       DASWBF010000372         7747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_693981,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000372 DASWBF010000000
VERSION     DASWBF010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693981"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VF874_13850"
     CDS             <1..612
                     /locus_tag="VF874_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9177106.1"
                     /translation="SPQQAVGLQPLACVLYALEQLPEIDGRHVGVIGQGSIGLLFSYA
                     AKANGARHVTGIDPVDRDVVGKEFGVDTVIRATSDRWVSHLEPNDKPDIVIEAVGHQV
                     ATLGHAIEATAFGGTVFYFGVADDDSYPISIRTMLRNNLTLKSGVTLDRRRVLRCADE
                     FAREHPELLKNYLTHTFAVDDVQAAFELACQPSPECIKIAIVE"
     gene            609..1361
                     /locus_tag="VF874_13855"
     CDS             609..1361
                     /locus_tag="VF874_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX9177107.1"
                     /translation="MTQSRLQQALAAREQVWGGWVVGPTVMGPEEFARTGYDYVGFDV
                     QHGYLDDADVALLLRRLEHVPIATAVRLPSSDPAPIGRVLDAGADAVIVAMIESADQA
                     AEAVAATRYGPAGVRSFGPLLPSLGHDPAALEARASVFVMVETAGGLSAVDDICSVPG
                     LSGVYVGPADLAISMGYGLGDAWTQPAVRDAMSLIQATASGANLVTGIHANDGKIGNA
                     MARMGFRMITLTSESQALRRGAAAHLKEATAE"
     gene            1379..2275
                     /locus_tag="VF874_13860"
     CDS             1379..2275
                     /locus_tag="VF874_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177108.1"
                     /translation="MPSAEELALLRQSVREMLQAISTSSPDKRWPDSWHANWDELDRQ
                     GLWSTLEPPDGSLAAAAVVVEELGRGLYPGPACEALAATCVSGVRDRRLAVFATDGVP
                     MVAVPPEALVITATAEGDLVAVPAGEVDSTTVPSLDVTRRIVAPRLHTQPATVMTAAD
                     SARRLLYCADTLGCVDHVVARTAEYAEQRTAFGSPIGKYQAVAHRLVDHAVTARQMRL
                     LLNGAIAAFDEQAADLDLQVATAETFFWGRATDVVSDCIQLCGAIAFTWEFGHHFYLR
                     RVVQNAAVGGRPHQRLAQESVW"
     gene            2278..3435
                     /locus_tag="VF874_13865"
     CDS             2278..3435
                     /locus_tag="VF874_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177109.1"
                     /translation="MAEFRQQVLDFIARKAPPGCWYDGIRVPRDADTERAVRNWYAEL
                     FSAGMIGGSWPVEHGGREDHTPYHDAVVMEEIVRARAPRPIDQVLLAAHLIIEFGSDE
                     QRARYLPRIRSGEDIWCQLFTEPGVGSDLARLTTKAVSLADGSFRLDGQKVWSTDAQW
                     SQMGVLLARTDPGVRPHAGLTVFLVDMTTPGIDVRPIREMTGSAEFCEVYLDGVIVPA
                     DAALGTVNCGWAVVNSALASERAYVGANAVMLELLFDDLVELARNVDYPAGSAIDDPV
                     VRDALADFSARVSAVQLIAADAVSRVAEDTDEPADGLIAKLVYTELNVAMCLYAVGLP
                     ASGRLRPDGHDVYSRWLQAFLWSRALTISGGSSEIIRNVLATQLFGLPRSW"
     gene            3437..3833
                     /locus_tag="VF874_13870"
                     /pseudo
     CDS             3437..3833
                     /locus_tag="VF874_13870"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            3830..4558
                     /locus_tag="VF874_13875"
     CDS             3830..4558
                     /locus_tag="VF874_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177110.1"
                     /translation="MTDRVALVTGAARGQGAAIVARLRADGFGVAACDLLVDELEAAV
                     DALGDAGVIAVPLDVTSGDQWAAAVNAVVDRFGSLTTLVNNAGVLHRASLAEETPGGF
                     ERSWRVNCLGPFLGIQAALDHLRRADGAAIVNTCSTGAIRAFPNHAAYGSSKWALRGL
                     TQIAAAELAPSGIRVNAVFPGPIETPMLDEATQARLAATSVSGRLGKPVEVADAVAFL
                     VSDHATFITGTELVVDGGQCLRIS"
     gene            4561..6099
                     /locus_tag="VF874_13880"
     CDS             4561..6099
                     /locus_tag="VF874_13880"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9177111.1"
                     /translation="MSARAKSRKHSRNDFSVGIIGAGPGGLALGIFLRKAGFRDFTIF
                     DRKDGVGGTWRINTYPGLACDVKSHLYSYSFDLNSNWSRLWPAQPEILEYFEECAQRY
                     NLEPNLKLHTEIRSAEWNSDTTKWCLTTATGEQHNFDIVVSAVGLFTQPILPNLVEEE
                     PFTGTVMHSAQWDHSVDLTDARVAVLGTGSTASQLLPQLAKVARKVYSVQRSATWILP
                     KPDRAYTDRERWVFAHVPFAKKFYRTRLWLRSEANISVIENGSDKTQEFKALAVKMLE
                     STVADEELRRKLTPDHPLGCKRLVFSSDFIPTLTQPHVEVVSSPAKALRARSLVTADG
                     TELDVDAVVCATGYAAADYLGQIGVVGEGGRSLRDTWGDGAYAYLGMAVPGFPNFFML
                     YGPNTNVGSNSVIFMLEAQAHYIVRALKYMRRKRKSYVAVRPDTMADFIAKIDQWMEG
                     TVWLTRCSNYFRAANGRVVTQWPRSARAIWTMTRRFNAKDYTFDPASAGHGVEADART
                     TATR"
     gene            6104..7030
                     /locus_tag="VF874_13885"
     CDS             6104..7030
                     /locus_tag="VF874_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9177112.1"
                     /translation="MDVSHRLDPALRDLASARTDLSLDALGPTRESLNDRRRETVRDI
                     DLSGVDVVDDRINTVPVRVYRAGPSPAPAVMYCHSGAFVLGNLDTDHRQCVEFARRAR
                     CTVISVDYRLAPENPCPAALDDALAVLVGVASQAILDVDASRIAVAGSSAGAALAAGL
                     AQRAAAGSAPPIVFQLLHQPVLDDRPTPSKDEFVTTPGVDGPATQLMWRHYLAEQPAT
                     DEAVPARKRELAGVAHAFISCSELDPLRDEALDYALRLMSAGVATELHVFPGTCHGFD
                     SLVPEWETSQLLFELQGRALDQAFSRGAQTLR"
     gene            complement(6994..>7747)
                     /locus_tag="VF874_13890"
     CDS             complement(6994..>7747)
                     /locus_tag="VF874_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HEX9177113.1"
                     /translation="ATIIGGAPVIAESLFAEADRRGLHALPLRCVAVGGAMVPQTVAD
                     TAIRFGVRQVRVYGSSEAPYSTSTALTDGSAVDDGVALSGVEVATRDGDELVIRGPHQ
                     FHGYLDAADNADAFSDDWFRTGDQADIVNGRVRITGRLKEVVIRKGMKISLAEVDTAA
                     AVLGECAAFGMPDEMCGERVVLAVRGGADITYDSVVERLSAVGLARWKLPEQIVLWDG
                     PFPLNASGKIVRHQLADSCARYRSVCAPRLNA"
BASE COUNT         1312 a         2578 c         2571 g         1286 t
ORIGIN      
        1 agtccgcagc aggcggtcgg actgcagcca ctcgcgtgcg ttctctacgc cttggagcag
       61 ctgcccgaaa tcgacgggcg gcacgtcggc gtcatcgggc agggatcgat cggtctgctg
      121 ttctcctacg cggccaaagc caacggcgcg cgccacgtca ccggcatcga cccggtcgac
      181 cgagacgtcg tcggcaagga gttcggcgtc gacacggtaa tccgcgcgac cagtgaccgg
      241 tgggtcagcc accttgaacc aaacgacaag ccggacatcg tgatcgaggc ggtcggccac
      301 caggtcgcca cgctgggcca cgcgatcgag gccaccgcgt tcggcggcac ggtgttctac
      361 ttcggcgttg ccgacgacga cagctacccg atcagcatac ggaccatgct gcgcaacaac
      421 ctgacgctga aatccggtgt gacactcgat cgtcgacgtg tcctgcggtg cgccgacgaa
      481 ttcgcccgtg agcatcccga actgctgaag aactatctca cccacacgtt cgccgtcgac
      541 gacgtgcaag cggcgttcga actcgcgtgc cagccgtcgc ccgaatgcat caagattgcg
      601 atcgtcgagt gactcagagc agattgcagc aagccttggc cgcccgggag caagtgtggg
      661 gtggatgggt ggtcggcccg accgtcatgg ggcccgagga attcgcccgg accggatacg
      721 actacgtcgg gttcgacgtg cagcacggat atctcgacga cgccgacgtc gcgctgctgc
      781 tgcgccgcct cgaacacgtc ccgatcgcca ccgcggtgcg actgccgtcg tcggatcccg
      841 caccgatcgg tcgcgtgctg gacgctggcg cggacgccgt catcgtcgcg atgatcgagt
      901 ccgccgacca ggccgccgaa gcggtggctg ccacccggta cgggcctgcc ggggtgcgga
      961 gcttcggccc gctgctgccg agcctgggtc atgacccggc cgccctcgaa gcgagggcca
     1021 gcgtgttcgt gatggtcgag accgcgggag gcctgtcagc cgtcgacgac atctgttcgg
     1081 tgccggggct ttcgggggtt tacgtcgggc ctgccgacct tgccatctcg atggggtacg
     1141 gcctgggcga tgcctggacc cagcctgcgg tgcgggacgc gatgtcgctc atccaagcga
     1201 ccgcgtcggg ggcgaacctg gtcaccggca tccacgccaa cgacggcaag atcggaaacg
     1261 ccatggcgcg gatggggttc cggatgatca ctctgacctc ggagtcgcag gccctgcgac
     1321 gcggcgctgc agcacatctc aaagaagcga ccgccgagtg aaccacacgg tgagtcacat
     1381 gccgagcgcg gaagagcttg cgctgctgcg tcagtcggtg cgcgagatgc tgcaggcgat
     1441 ctccacctcg tcgccagaca agcgatggcc tgactcctgg cacgccaact gggacgagct
     1501 cgatcggcag ggattatggt cgacgctcga gccacccgac ggatcgctgg cagccgccgc
     1561 ggtggtggtc gaggaactcg gtcgtgggct ctacccgggt ccggcgtgcg aggcgcttgc
     1621 cgccacgtgc gtgagcggtg tccgtgaccg ccggctcgcg gtgtttgcca cggacggcgt
     1681 tccgatggtc gcggtcccac ctgaagcgtt ggtgatcacg gcgacggctg agggggacct
     1741 ggtagcggtg ccggccggtg aagtcgacag caccaccgtg ccgtcactcg atgtcacccg
     1801 ccggatcgtc gcgccgcgat tgcacacgca gccagccaca gtgatgactg ctgccgactc
     1861 cgcacggcga ctgctgtatt gcgccgacac cctcgggtgt gtcgaccacg ttgtcgcgcg
     1921 cacggctgaa tatgccgagc agcgaacggc attcgggtca ccgatcggta agtaccaagc
     1981 cgtcgcgcac cgcctcgtcg accatgcggt caccgcccgg cagatgcggc ttctgttaaa
     2041 cggggcgatc gcggcgttcg acgagcaggc cgccgacctg gacctccagg tggcgacggc
     2101 agaaacgttc ttctggggcc gcgccaccga cgtcgtcagc gactgcattc agttgtgcgg
     2161 agccatcgcg ttcacgtggg agtttggcca ccacttctac ctgagacgcg tggttcagaa
     2221 cgccgctgtc ggcggacggc cgcaccaacg gctggcgcag gagtcggtgt ggtagacgtg
     2281 gccgagttcc gtcagcaggt tctcgacttc atcgcgcgca aagccccgcc ggggtgctgg
     2341 tatgacggga ttcgggtgcc ccgcgacgcc gacaccgaac gcgcggtgcg caattggtac
     2401 gccgagttgt tcagtgccgg catgatcggc ggctcgtggc cggtggaaca cggtggccga
     2461 gaggatcaca ccccctacca cgacgcggtg gtcatggagg agatcgtgcg cgcgcgagcc
     2521 ccgcggccca tcgaccaggt actgctggcg gcacacctga tcatcgagtt cggctccgac
     2581 gagcagcgag cccggtatct tccacgcatc cgatcgggtg aggacatctg gtgccaactg
     2641 ttcaccgaac ccggtgtggg cagcgacctg gccaggctga ccaccaaggc ggtgtcgttg
     2701 gcggacggga gctttcgact cgacggccag aaggtgtgga gcaccgacgc gcaatggtcc
     2761 caaatggggg tgctgctggc gcgcacggat ccgggcgtac gaccgcacgc cgggctcacc
     2821 gtgttcttgg tcgacatgac gaccccaggc atcgacgttc ggccgatccg ggagatgacg
     2881 ggttctgccg agttctgcga ggtgtatctc gacggcgtga tcgtgcctgc cgacgccgcc
     2941 ctcggcaccg tgaattgcgg ttgggccgtg gtgaattcgg ccttggcctc agagcgggcc
     3001 tacgtcggtg ccaacgccgt gatgctcgag ttgctgttcg acgacctcgt cgagctcgcg
     3061 cgcaatgtcg actatcccgc cggcagcgcg attgacgacc cggttgtgcg agacgccctg
     3121 gcggacttca gcgcccgcgt ctccgcagtg cagctgatcg ccgccgacgc ggtcagtcgg
     3181 gtggccgaag acaccgacga gccggccgac ggcctgatcg ccaaactcgt atacaccgag
     3241 ctgaacgtcg cgatgtgcct ctacgcggtc ggcctgcccg cttcgggccg gctacgtccg
     3301 gacgggcacg acgtgtattc ccgatggctg caggcgttcc tgtggtcgcg cgcgctgacg
     3361 atctcgggcg gctcgtcgga gatcatccgc aacgtgctcg ccacgcaact gttcgggctg
     3421 ccgaggtcgt ggtgacatga cgcctcaagc tgcgatccgc gagctgctcg ccgcctacgc
     3481 gctcacactg gacgccgacg acatcgacgg ctgtctggcg ttgttcacga acgacgccga
     3541 gttcgtggtg ttcggcaaga cgttgtccgg ccccaaccgg atacgcaaaa atgctcaccc
     3601 gcgcaccgcg gggaatgcat ttgaccggag ccacgctcgt cgacgtcgat gacctcaccg
     3661 cgacggtgcg ctctcaggtg ctgttcgtcg actcgaccac tcaccaactg cgaccggcgc
     3721 tgtatgacga cgaggtggtc aacctggacg gacggtggcg tttccggcgc cgccgttgcc
     3781 aattcctcac ggtggcaggg ctttccgata acccgcagga gcaggtgcag tgacagatcg
     3841 tgtcgcgctg gtcaccggcg ccgcccgcgg gcagggcgcg gccatcgtcg cacggctgcg
     3901 cgcagacggg ttcggcgtgg ccgcatgcga tctgctggtc gacgaactcg aggctgccgt
     3961 cgacgcactc ggcgatgccg gggtgatcgc ggtcccgctg gatgtgacgt ccggcgacca
     4021 gtgggcggct gcggtgaacg cggtcgtcga ccggttcggc tcgttgacca cgctggtgaa
     4081 caatgccggt gtgctgcacc gcgcctcgct ggccgaggag acgccgggcg gtttcgagcg
     4141 cagctggcgg gtcaactgcc tgggtccgtt cctcggcatc caggctgcgc tcgaccatct
     4201 ccgccgagcc gacggcgccg cgatcgtcaa cacgtgcagc accggggcta tcagagcctt
     4261 tccgaaccat gccgcctacg gctcgtcgaa gtgggcgcta agaggtctca cgcagatcgc
     4321 cgccgccgaa ctcgccccgt ccggaattcg ggtgaacgcc gtgttccccg ggcccatcga
     4381 gaccccgatg ctcgacgagg cgacccaggc ccgccttgcc gccacgtcgg tatccggccg
     4441 cctcggcaag cccgtcgagg tggccgacgc ggtggctttc ctcgtctcag accatgcaac
     4501 gttcatcacg ggcaccgaac tcgtcgtgga tggaggacaa tgcctgcgaa tcagttagcg
     4561 atgagcgctc gcgcgaagag cagaaagcac agtcgcaacg acttctcggt gggaatcatc
     4621 ggggccggcc cgggcggctt ggccctcgga atattcctga gaaaagccgg gttccgcgac
     4681 ttcacgatct tcgaccgaaa ggacggggtc ggcgggacgt ggcggatcaa cacctacccc
     4741 ggtctggcct gcgacgtgaa gtcgcacctg tattcgtact cgttcgacct gaattcgaac
     4801 tggtcgcggc tgtggccggc gcagcccgag atcctggagt acttcgagga gtgcgcccag
     4861 cggtacaacc tcgagccaaa cctgaagctt cacaccgaaa tccgctccgc cgagtggaac
     4921 tccgacacaa ccaaatggtg cctgacgacc gccacgggag aacagcacaa cttcgacatc
     4981 gtggtgtccg ccgtaggcct gttcacccaa ccgattcttc cgaatctggt cgaagaagaa
     5041 ccgttcaccg ggaccgtgat gcactcggcg cagtgggatc actctgtcga cctgaccgac
     5101 gcccgtgtcg ccgtgctggg aaccgggtcg accgcgtcgc agctgctgcc ccaactggcg
     5161 aaggtggcca ggaaggtcta ctcggtgcaa cggtcggcga cctggattct gccgaaaccg
     5221 gaccgcgcct ataccgaccg ggagcgttgg gtgttcgcgc atgtaccgtt cgccaagaag
     5281 ttctaccgga cacggctgtg gctgcgcagc gaggccaaca tttcggtgat cgagaacggc
     5341 agcgacaaaa ctcaagagtt caaagcgctc gccgtgaaga tgctcgaatc caccgtggcc
     5401 gacgaggaac tgcgccgcaa actcactccg gaccacccgc tgggctgcaa acggctggtg
     5461 ttctcgtcgg acttcatccc gacgttgacg caaccgcacg tcgaagtggt gtccagcccg
     5521 gccaaggctc tgcgtgcgcg gtcgttggtg accgcggacg gcaccgaact cgacgtcgac
     5581 gcggtggtct gcgcgacggg gtatgcggcg gcggattacc tcggccagat cggcgtcgtc
     5641 ggtgagggcg gccggtcact gcgggacacc tggggcgacg gagcatacgc atatctgggc
     5701 atggccgttc cgggtttccc gaacttcttc atgctgtacg ggccgaacac gaatgtcggc
     5761 tccaacagcg tcatcttcat gctcgaggcg caggcgcact acatcgtgcg ggcgctgaag
     5821 tacatgcggc gcaagcgcaa atcctatgtc gcggtgcgac cggacacgat ggccgacttc
     5881 atcgcgaaaa tcgaccagtg gatggagggc accgtgtggt tgacgcggtg cagcaactac
     5941 tttcgcgccg ccaacggccg cgtagtcacc cagtggccgc gcagcgcacg cgcgatctgg
     6001 accatgacac gcaggtttaa cgcaaaggat tacaccttcg atccggcatc cgccggacac
     6061 ggcgtcgagg ccgacgcgcg gacaaccgcg acccggtagc cctgtggacg tgtcgcaccg
     6121 gctcgacccg gcgttgcggg acttggcctc ggcgcgcacc gatttgtcgc tcgacgcgct
     6181 gggcccgacc cgcgagtcgt tgaacgaccg tcgccgcgaa accgtccgag acatcgacct
     6241 ttccggcgtc gacgtggtcg acgaccggat caacaccgtg ccggttcggg tctaccgcgc
     6301 cggcccgtca cccgcaccgg cggtcatgta ctgccactcc ggagccttcg tcctgggaaa
     6361 tctcgatacc gaccaccgcc agtgcgtgga gttcgcgcgg cgtgcccgct gcacggtgat
     6421 ctcagtcgac taccggttgg cgcctgaaaa cccctgcccg gcagcgcttg acgatgcgct
     6481 ggcggtactg gtgggggtcg cctcccaggc aatcctcgac gtcgatgcga gtcggatcgc
     6541 cgtggcgggc agcagcgcgg gtgccgcgct ggccgcgggc ctggcgcagc gcgccgccgc
     6601 cggatcggcg ccgccgatcg tgttccaact gctgcaccaa cccgtgcttg acgatcgccc
     6661 aacaccgtcg aaggacgaat tcgtgacgac cccgggtgtc gacggtccgg cgacacagct
     6721 gatgtggcgc cactatctgg ccgaacaacc ggcgaccgac gaggctgtgc ccgcgcgcaa
     6781 gcgcgagttg gccggtgtgg cacacgcgtt catcagctgc tcggagctgg acccgctgcg
     6841 cgacgaagcg ctcgactatg cgctgcgcct gatgtcggcc ggtgtcgcca ccgaactgca
     6901 cgtcttcccg gggacgtgcc acggattcga ttcactggtg ccggagtggg agacgagcca
     6961 gttgttgttc gagcttcagg gacgagcgct cgatcaggca ttcagccgtg gcgcgcagac
     7021 gctgcggtaa cgcgcgcagg agtcggccaa ttggtgacgc acgatcttgc cgctggcgtt
     7081 cagcggaaac ggcccgtccc ataagacgat ctgttcgggc aacttccacc tggcgagccc
     7141 caccgccgac aaccgttcga ccacgctgtc gtaggtgatg tccgcgccgc cgcggacagc
     7201 cagcaccacg cgttcgccgc acatctcgtc cggcataccg aatgcagcac attcacccaa
     7261 caccgcagcg gcagtgtcga cttcggccaa cgagatcttc attcccttac ggatgactac
     7321 ctccttgagc cggcccgtga tcctgactcg gccattgacg atgtccgcct ggtcaccggt
     7381 gcggaaccaa tcgtctgaga acgcgtcggc gttgtcggcg gcatcgaggt aaccgtggaa
     7441 ctggtgcggc ccgcggatga cgagctcgtc gccgtcacgg gttgcgacct cgaccccgga
     7501 gagtgcgacg ccgtcatcga ccgctgaccc gtccgtcaac gccgtcgacg tggaatacgg
     7561 cgcctccgag gagccgtaca ccctgacctg tcgaacaccg aaccgtatcg cggtatcggc
     7621 caccgtctgc gggaccatcg cgccgccgac cgcaacgcac cgcagcggca gagcatgaag
     7681 gccccgccgg tcagcttcgg cgaacagcga ttccgcgatg accggggctc cgccgatgat
     7741 cgtcgca
//
LOCUS       DASWBF010000373        11030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_886024,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000373 DASWBF010000000
VERSION     DASWBF010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886024"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF874_13895"
     CDS             <1..502
                     /locus_tag="VF874_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX9177114.1"
                     /translation="TPPPPPNEPPPSGYQPPPPGYPAPPPMPYAGQAPPPAAPRNGLG
                     VAALVVAIIGLLVAWIPFFGLLGIVLGIAAVILGFIGRGRAKRGEANNGGVALAGILL
                     GILAVVVAVASTVIAAFVVKEVGGGDYIDCLNKAGNDTSAQQQCADEFRQHLETQFSV
                     TLTTAP"
     gene            complement(504..1895)
                     /locus_tag="VF874_13900"
     CDS             complement(504..1895)
                     /locus_tag="VF874_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX9177115.1"
                     /translation="MTEPVKVARVRARERVVVDVDRPAARWIGALAVLSLFCWLVVLL
                     ARNHDHADWNAAGRLAWSLTILTGVALIARGIFLGRPVTAAHATSAMVVLLVGLGAHL
                     LSYDLLGNVLVAGAGLVLMWPTTARPQPEDLPRVWSLVNTTQGDPLAPFALQSLKSYC
                     FNDAGTAAIAYRTRIGFATVSGDPIGDETQFPQLVADFVAMCHTRGWRILVLGCSERR
                     LPLWRNDAVVGQRMRRVPIGRDVVIDVRRFDMVGRKYRNLRQAVQRTHNFGITTEVVA
                     EQDVDDALRAELAQVLFASHSGARTERGFSMILDGALERRYPGIQLIIARDRDGQVQG
                     FHRYASSGGGTDITLDVPWRRPGAPNGIDERLSIDMIYEAKSFGAQRLSLAFAAFPEV
                     FKNKDRNRLQDLVYHAIHLLDSLIALESLYRYLRKFHALGDRRYVLLSLRHIMPAVVV
                     LLSLEFAPRRRHL"
     gene            complement(1906..2781)
                     /locus_tag="VF874_13905"
     CDS             complement(1906..2781)
                     /locus_tag="VF874_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX9177116.1"
                     /translation="MRSDFEQLLAILDLNRVDDSTFTGSHPTKTPTRTFGGQLMAQSV
                     VAASRTVATGLPLASLTVTFINGGDPTADIEYRVVNLRDERRFANRRVDAVQDGNLLA
                     SAMVSYLSGGRGLEHGVDAPEVVAPESLPPIGELLRGYEDTVPGFVKALRPIEWRYTN
                     DPAWVMRKKGERLPYNRVWMKADGRMPDDPLLHSAAMVYSSDTTVLDSIITTHGLSWG
                     FDRIFAVTVNHSVWFHRQVNFGDWVLYSTTSPVAADSRGLGTGYFFDRHGGLLATVVQ
                     EGIVKYFPAHGADHA"
     gene            complement(2789..4207)
                     /gene="pyk"
                     /locus_tag="VF874_13910"
     CDS             complement(2789..4207)
                     /gene="pyk"
                     /locus_tag="VF874_13910"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9177117.1"
                     /translation="MNRRAKIVCTFGPATNSDEVVRSLVEAGMDVARMNFSHGDHADH
                     KATYDRVRAASDVTGRAVGVLADLQGPKIRLGRFAAGHAFWQSGDTVRITVEDCEGTH
                     DRVSTTYRQLAHDAAPGDRLLVDDGKVVLIVEGIDGDDVVCTVTEGGPVSNNKGLSLP
                     GMSVSVPALSEKDIEDLEFALRLGVDLVALSFVRSPADIELVHDVMDRVGRRVPVIAK
                     LEKPEAIDNLEAIVLAFDAVMVARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVAT
                     QMLESMVENLRPTRAEASDVANAVLDGADALMMSGETSVGKHPVEAVKTMARIITAVE
                     QNSTAAPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPL
                     LAFTDRPEVRSQLAMTWGTETFIVPHMVATDDMIRQVDKSLLGLGRYKRGDLVVIVAG
                     APPGTVGSTNLIHVHRIGEDDH"
     gene            complement(4239..5741)
                     /locus_tag="VF874_13915"
     CDS             complement(4239..5741)
                     /locus_tag="VF874_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX9177118.1"
                     /translation="MADPTGFLRVPKVEAPKRPVDERVGDWHEVYERQSPQARAAEVS
                     QQARRCMDCGIPFCHSGAAGCPLGNLIPEWNDLVRRGRWDAASERLHATNNFPEFTGR
                     LCPAPCESACVLSIGEEKTGGSVTIKRIENTIADQAWHLGIVAPQPAAIATGKRVAVV
                     GSGPAGLAAAQQLTRAGHEVTVYERDDRIGGLLRYGIPEYKMEKATLNRRLAQMRSEG
                     TRFVTGCEAGVDLPVEELRERHDAVVLAVGALRARDYHVEGRDLDGVHLAMDHLVPGN
                     KECEGDGPSPVTAKGRHVVIIGGGDTGADCLGTAHRQGALSVTQLDYNPEPPEVRDEW
                     RSPWPTWPLVLRTSPAHAEGGHRHYQVAVQRFCGDEHGHLRAIEIAEVKVARDADGRR
                     VITPVGDSLELPCDLALLAIGFEGVECMPLLDELGLDLNRGGALPCGPDWQTTAPGVF
                     VCGDAHRGASLIVWAIAEGRSAAHAVDAYLMGESDLPAPVRPSALPLAVV"
     gene            complement(5734..10287)
                     /gene="gltB"
                     /locus_tag="VF874_13920"
     CDS             complement(5734..10287)
                     /gene="gltB"
                     /locus_tag="VF874_13920"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX9177119.1"
                     /translation="MLFSALPEPQGLYDPAHEHDSCGVAVVADIRGRRSHAILADGLL
                     ALEHLDHRGAAGAETNSGDGAGVLLQLPVELFQEVVEFELPPPTLDGCNTYAAGLCFL
                     PQDPVARAKAQSCVESIAADEGLEIRGWRDLPVDPDGADVGMTALKCMPYMSQLFVTA
                     PEHDGIRPGGIDLDRRAYPLRKRADRTGVYFPSLSSRTISYKGMLTTVQLPQYFPDLR
                     DQRCMTAIAIVHSRFSTNTFPSWPLAHPFRFIAHNGEINTVRGNRNRMHAREAMLNSA
                     MIPGDLSRLSPICPPESSDSASFDQVLELLHLGGRGLPHAVMMMIPEAWENNTTMDPA
                     LRDFWKFHASIMEPWDGPACLTFTDGTVVGAVLDRNGLRPGRWWRTLDDRVILASESG
                     VLDIPSAEVVAKGRLEPGRMFLVDTAAGRIVSDDEIKRELAQTYPYGEWLHAGLLDLS
                     ALPDRTRVQPNHESIAHRQVAFGYTEEDLRILLTPMAASGTEPLGSMGTDTPPAVLSQ
                     RPKLFYDYFVELFAQVTNPPLDAIREEIVTSLARVMGPEQNLLEPSAASCRQILLRWP
                     VLDNDELNKIVHINDDGDHPGRKATVLRALYDVERGGDGLAGAIDDLGNRACEAIADG
                     ARTLVISDRDSDHTRAPIPSLLAVAAVHHHLVRTNQRTSVALVVESGDAREVHHIALL
                     IGYGAAAVNPYLAFESIEDLIREGELTGIETVAAVRNYLTALCKGVMKVMSKMGISTV
                     GSYTGAQAFEAIGLDMTVVDEYLTGTPSKLGGVGLDVIAEEVRQRHRRAYPDNPTERV
                     HRRLEVGGEYAFRREGELHLFTPETVFLLQHSTRTGREDIFRQYSDEVDRLSREGGTL
                     RGLFEFKRGERPPVPLEEVESVESICSRFNTGAMSYGSISAEAHETMAIAMNTIGGRS
                     NSGEGGEDVDRLYDPRTRSAVKQVASGRFGVTSDYLVNATDIQIKMAQGAKPGEGGQL
                     PGFKVYPNIAKTRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANDKARIHVKLVS
                     SVGVGTVAAGVSKAHADVVLISGYDGGTGAAPLTSLKHAGAPWEIGLADTQQTLVLNG
                     LRDRITVQADGGMRTARDVVVATLLGAEEFGFSTAPLVVAGCIMMRVCHLDTCPVGVA
                     TQNPQLRARFNGKPEFVENFFRFVAADVRRYLAELGFRSVDEAVGHAEMLDTAEGVAH
                     WKSRGLDLTPIFTVPTDGHGGPTQRRKLRDQCHALDQALDQTLIALAEGALEDARRVR
                     LELPVRNVNRTVGTLLGAEVTRRYGAQGLPDGTINVALTGSAGQSLGAFLPPGITLEL
                     TGDANDYVGKGLSGGRVIVKPPDDVLFLPEDNVIAGNTLLYGATSGEVYLRGRVGERF
                     CARNSGAVAVAEGVGDHACEYMTGGHVVVLGQTGRNMAAGMSGGIAYVLGLNPAKVNT
                     AMVKLQDLDPEDLIWLREVVANHARHTGSAVARSVLSDWPRRSAQFTKIMPTDYERVL
                     AATRMAKAEGRDVDTAIMEASRG"
     gene            complement(10396..10809)
                     /locus_tag="VF874_13925"
     CDS             complement(10396..10809)
                     /locus_tag="VF874_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX9177120.1"
                     /translation="MALDSARSQRGKAYTALATGAVLAGAVAYVGINDPHRPGALFPP
                     CPFKLLTGWNCPACGGLRMTHDLLHANLSAAVVDNIVLLVGLPALLGWFLWRRHTGRP
                     ALTRAAVVVIITTAIAWTVIRNMPGFPLVPTLYSG"
     gene            complement(10799..>11030)
                     /locus_tag="VF874_13930"
     CDS             complement(10799..>11030)
                     /locus_tag="VF874_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEX9177121.1"
                     /translation="SDKSKLVAGLLQLLGLFGIVGVGRMYLGEVGLGVAQLLVGIFTC
                     FIGAWIWGVIDAILILTDNVRDPQGRPLRDGT"
BASE COUNT         1845 a         3621 c         3681 g         1883 t
ORIGIN      
        1 gacaccgcca cctccgccca acgagccacc gccgtccggt tatcagcccc cgcctcccgg
       61 ttacccggcg ccgccgccga tgccgtacgc gggtcaggca ccgcccccgg ccgctccgcg
      121 caacgggttg ggcgttgccg cgttggtggt cgcgatcatc ggcctgctgg tcgcctggat
      181 accatttttc ggcttgcttg ggatcgttct cgggatcgcc gctgtcatct tgggattcat
      241 cggtcgggga cgcgccaaac gaggcgaggc caacaacggc ggcgtcgcac tcgcgggaat
      301 cctgcttgga atcctggcgg tcgtcgtggc ggtggcttcc accgttatcg cggctttcgt
      361 cgtcaaagag gttggtggcg gcgactacat cgactgcctg aacaaggccg gtaacgacac
      421 gagcgcgcag cagcagtgcg ccgacgagtt ccgtcaacac ctggagacgc agttcagcgt
      481 cacgctgacg accgcgccct gagtcacagg tggcgtcggc gcggcgcaaa ctccagcgac
      541 aacagcacga cgacagccgg catgatgtgc cgcagcgaca gaagtacgta acgccggtcg
      601 ccgagcgcgt gaaacttgcg cagataccgg tacagggatt ccagcgcgat cagcgaatcg
      661 agcagatgga ttgcgtggta gacaaggtct tgaaggcgat tgcggtcctt gttcttgaag
      721 acctccggaa aggccgcgaa tgccagtgac aaccgttgcg cgccaaacga tttagcttca
      781 tagatcatgt cgatggacag gcgttcatcg atgccgttgg gtgcacctgg ccggcgccac
      841 ggcacatcga gggtgatgtc cgtgccgccg cccgagcttg cgtaccggtg aaacccttgc
      901 acctgtccgt cgcgatcgcg cgcgatgatg agctgaatgc ctggatagcg gcgctcgagt
      961 gccccgtcga ggatcatcga gaatccccgt tcggtgcgcg cgccgctgtg cgacgcgaac
     1021 aacacctgcg ccagctcggc ccgcagcgca tcgtcgacat cctgctcggc gaccacctcg
     1081 gtggtgatgc cgaagttgtg cgttcgctgc acagcctggc gcaggttgcg gtacttgcgt
     1141 ccgaccatgt cgaatcgccg cacgtcgatg acgacgtcac gcccgatcgg caccctgcgc
     1201 attctctggc ccaccacggc gtcgttgcgc cacaacggca accgtcgctc gctgcagccg
     1261 agcacgagga tgcgccagcc gcgggtatgg cacatcgcaa cgaagtcggc gaccagctga
     1321 gggaattggg tctcgtcacc gatcgggtca ccgctcaccg tcgcgaaacc gatacgcgtg
     1381 cggtatgcga tcgccgcggt gccggcatcg ttgaagcagt agcttttcag cgactgcagc
     1441 gcgaacgggg ccagcgggtc gccctgggtc gtgttcacca gactccacac ccgaggcaga
     1501 tcctcgggct gcggccgcgc ggtcgtcggc cacatcaaca ccagaccggc gccggcgacg
     1561 agcacattgc cgagaaggtc atacgacaac aggtgcgcgc cgagaccgac gagaagcacg
     1621 accatcgcag acgtcgcgtg cgcggccgtc accggacggc cgaggaaaat cccgcgggcg
     1681 atcagcgcga cgccggtcaa gatcgtcaac gaccaggcga gccggcccgc ggcgttccag
     1741 tcggcatggt cgtgattgcg ggcgagcagc acgacgagcc aacagaacag gctcagaacg
     1801 gctagcgcac cgatccaccg cgctgccggt ctgtcgacgt cgacgacaac acgttcgcgg
     1861 gcgcggaccc tcgcgacttt gaccggttcc gtcacgagtt aaagattacg cgtggtcagc
     1921 gccgtgagcc ggaaaatact tcacgattcc ctcctgtacg acggtggcga gaagcccgcc
     1981 gtgacggtcg aagaagtagc cggtgcccag tcctcgggag tccgccgcca ccggcgacgt
     2041 cgtcgaatac agcacccagt cgccgaagtt cacctggcgg tgaaaccaga ccgaatggtt
     2101 cacggtgacc gcgaagatcc tgtcgaatcc ccaggacagg ccgtgtgtgg tgatgatcga
     2161 gtccagaacg gtggtatcgg aggagtacac catggctgcg ctgtgcagca gcgggtcgtc
     2221 gggcatcctg ccgtcggctt tcatccacac ccggttgtag ggcagtcttt cgcccttctt
     2281 gcgcatcacc caggccgggt cgttggtgta gcgccactcg atgggccgca acgccttgac
     2341 gaagcctggg acggtgtctt cgtagccgcg aagcagctcc ccgatcggcg gcaacgactc
     2401 gggggcgacg acctccggag cgtccacacc atgctcgagg ccacggccgc ccgacagata
     2461 cgaaaccatt gctgacgcaa ggagattgcc gtcctgcaca gcgtcgacgc gccggttggc
     2521 gaatcggcgt tcgtcgcgca ggttcacgac gcggtactcg atgtcggccg tcgggtcgcc
     2581 gccgttgatg aaagtcacgg tcaatgatgc gagtggaagc ccggtggcca ccgtgcgtga
     2641 ggcggcgacg acggactgcg ccatcagctg cccaccgaag gtgcgggtcg gggttttcgt
     2701 ggggtgggag ccggtgaagg tgctgtcatc gacgcggttg aggtcgagga tggcgagcag
     2761 ctgctcaaaa tccgatcgca cgatccccct agtggtcgtc ctctccgatc cggtgcacgt
     2821 ggatcaggtt ggtcgagcct actgtgcccg gcggagcgcc cgcgacgatg accaccaggt
     2881 caccgcgttt gtagcggccc agcccgagca gcgacttgtc cacctggcgg atcatgtcgt
     2941 cggtggccac catgtgcggc acgatgaacg tctcggtacc ccaggtcatc gccagctgac
     3001 tgcggacctc gggccgatcg gtgaacgcca gcaacggcag tggagtgtgc agccgtgcca
     3061 gccggcgcac ggtgtcgccg gactgcgtga acgccaccaa cgccttggcg tccaggcgtt
     3121 ccccgatgtc gcgcgcggcg taggagatca ccccgcgctt ggtgcgcggc acgtgagtca
     3181 agggcggcgc ggccgtcgaa ttctgttcga ccgccgtgat aatccgcgcc atcgtcttga
     3241 cggcttcgac cgggtgcttg cccaccgacg tctcccccga catcatcagc gcatcggcac
     3301 cgtcgagcac cgcgttcgcg acatcggagg cctcggcacg ggtgggccgc aggttctcga
     3361 ccatcgactc cagcatctgg gtcgcgacga tcacgggttt ggcgttctcc ctggccattt
     3421 ggatcgcacg cttctgaacc agcggcactt cctcgagcgg cagttccact cccagatcgc
     3481 cacgagcgac catcaccgcg tcgaatgcga gcacgatcgc ttccagattg tcgatggcct
     3541 cgggcttttc gagcttggcg atgacgggca cgcgccggcc gacgcggtcc atcacgtcgt
     3601 gcaccagttc gatgtcggcg ggtgagcgga caaacgacag cgccaccagg tcgacaccca
     3661 gccgcagcgc gaactcgaga tcctcgatgt ctttctccga cagcgccggc accgacacgc
     3721 tcatgccggg tagcgacaga cccttgttgt tcgacaccgg gccgccctcg gtgaccgtgc
     3781 acactacgtc gtcgccgtcg attccctcga cgatcagcac gaccttgccg tcgtcgacga
     3841 gcaagcggtc gcccggcgcg gcgtcgtgag ccagctgcct gtacgtggtg gacacccggt
     3901 cgtgcgtgcc ctcgcagtcc tcgacggtga tacgcaccgt gtcgccgctc tgccagaacg
     3961 cgtgcccggc agcgaaccgg ccgagccgga tcttggggcc ctggagatcg gcgagcacac
     4021 ccaccgcgcg gccggtcaca tcggaggcgg cgcggacccg gtcgtaggtc gctttatggt
     4081 cggcgtggtc gccgtggctg aaattcatgc gggccacgtc catgccggcc tcgacaagcg
     4141 accggaccac ctcgtcggag ttggtggcag ggccgaatgt gcagacgatc ttcgcgcgtc
     4201 tattcacgaa ttccgagcat agtcctggcg aacgtccttt acacgaccgc gagcggcaac
     4261 gccgaaggcc gcaccggcgc gggcagatcc gactcgccca tcaggtaggc gtcgactgcg
     4321 tgcgcggcgc ttcgcccttc cgcgatggcc cacacgatca gcgacgcgcc gcggtgggcg
     4381 tcgccgcaga cgaacacgcc gggcgcggtg gtctgccagt ccggcccgca gggcaacgcg
     4441 ccgccgcggt tgaggtcaag cccaagttcg tcgagcagcg gcatgcattc gacgccctcg
     4501 aagccgatgg ccagcaacgc gagatcgcag ggcaactcga ggctgtcccc gaccggcgtg
     4561 atgacgcgac gcccgtcggc gtcgcgagcg actttgactt cggcgatttc gatggcccgc
     4621 aggtgtccgt gctcgtcgcc acagaaccgc tggaccgcga cctgatagtg gcggtgaccg
     4681 ccctccgcgt gggcaggcga ggtgcgcagc accagcggcc acgtcggcca aggcgatcgc
     4741 cactcgtcgc gaacttcggg cggttcgggg ttgtagtcga gttgggtgac cgacaacgcg
     4801 ccctgccgat gcgcggtacc caggcagtcc gctccggtgt cgccgccgcc gatgatcacc
     4861 acatgcctgc ccttggccgt gacgggtgac ggcccgtcgc cctcgcactc tttattgccg
     4921 ggaacgaggt ggtccatcgc caggtgcacg ccgtcgaggt ctcgaccctc gacgtggtaa
     4981 tcgcgggctc gtaatgcccc gaccgcgagc accaccgcgt cgtgccgctc acgcagctct
     5041 tcgacgggca ggtcgacgcc cgcctcgcag ccggtgacga aacgtgttcc ctcggagcgc
     5101 atttgggcga gccgccgatt caacgtcgcc ttctccatct tgtattccgg gattccgtag
     5161 cgcagcagcc ccccaattcg gtcgtcgcgt tcgtagacgg tgacctcatg gccggcacgc
     5221 gtgagttgtt gcgccgccgc gagtcccgcg ggtccggatc cgaccacggc gacccgctta
     5281 cccgttgcga tcgcggccgg ttgcggcgcg acgattccga ggtgccaggc ttggtcggcg
     5341 atcgtgtttt cgatgcgttt gatcgtgacg ctgccgccgg tcttctcctc accgatcgac
     5401 agcacgcacg cggactcgca cggtgccggg cacagccggc cggtgaactc ggggaagttg
     5461 ttggtggcgt gcagccgctc gctggcggcg tcccagcggc cccgccgcac caggtcgttc
     5521 cattcgggaa tgaggttgcc caacggacag cccgcagccc cggagtggca gaacgggatt
     5581 ccgcagtcca tgcagcgccg cgcctgctgc gacacctccg cggcgcgcgc ctgcgggctc
     5641 tgccgctcgt agacttcgtg ccagtccccc acacgctcgt cgaccgggcg cttcggcgcc
     5701 tcgaccttgg gcactctcag aaatcctgtc ggatcagcca cggctggcct ccatgatcgc
     5761 ggtgtcgacg tctctgcctt cggccttcgc catccgtgtg gcggccagca cccgctcata
     5821 gtcagtgggc atgatcttgg tgaattgagc gctgcgcctc ggccagtcgg agagcaccga
     5881 ccgtgcgacg gcgctcccgg tgtggcgggc gtggttggcg acgacctcgc gcagccagat
     5941 cagatcctcg gggtcaagat cctgcagctt gaccatcgcg gtgttgacct tggcagggtt
     6001 gagcccaagc acgtaggcga tgccgccgga cattcccgcc gccatgttgc gtccggtttg
     6061 gccgagcacc accacatgac ctcccgtcat gtactcgcat gcgtggtcgc cgacgccctc
     6121 cgcgaccgcc accgcgccgg agttgcgggc gcagaagcgt tctccgactc ggccgcgcag
     6181 gtacacctcg ccggacgtgg caccgtacag cagcgtgttt ccggcgatga cgttgtcctc
     6241 gggcaagaac agcacgtcgt cgggtggctt gacgatcacc cgtccacccg aaagtccctt
     6301 gccgacatag tcgttggcgt cgccggtgag ctccagcgtg atgcccggcg gcaggaatgc
     6361 gccgagcgac tggccggctg aaccggtgag cgcgacattg atggtgccgt cgggtaggcc
     6421 ctgcgcgccg tagcggcggg tgacctccgc accgagaagt gttccgacgg tgcggttgac
     6481 gttgcggacc ggcaactcca gtcgtacccg gcgggcatcc tcaagtgcgc cctcggcgag
     6541 cgcgatgagg gtttggtcga gcgcctggtc cagcgcgtgg cactggtctc ttagcttgcg
     6601 ccgctgcgtc ggcccgccgt ggccatcggt gggcaccgtg aagatcggcg tcaggtccaa
     6661 ccccctgctc ttccagtgcg ccacgccctc ggcggtgtcg agcatctcgg cgtggccaac
     6721 cgcctcgtcg acgctgcgga aaccgagttc ggcgagatag cgccggacgt ccgcggcgac
     6781 gaaccggaaa aagttctcga cgaactccgg tttgccgttg aaccttgccc gcagttgcgg
     6841 gttttgggtg gcgacgccca ccgggcaggt atcgaggtgg cagacccgca tcatgatgca
     6901 gccggcgacg accagcgggg ctgtcgagaa gccgaactcc tcggcgccga gcagcgtggc
     6961 gaccaccacg tcacgcgccg tgcgcatgcc gccgtcggcc tggacggtga tcctgtcacg
     7021 taaaccgttg agcaccaagg tctgctgggt gtccgcgagc ccgatctccc acggcgctcc
     7081 ggcatgcttg agcgaggtca gcggcgcggc gccggtaccg ccgtcgtagc cggagatcag
     7141 caccacgtcc gcgtgggcct tcgacacgcc ggccgcgacc gtgccgacgc cgaccgagct
     7201 caccagcttg acgtggattc gcgctttgtc gttggcgttc ttgaggtcgt ggatgagctg
     7261 cgcgagatcc tcgatcgagt agatgtcgtg gtgcggcggc ggcgagatca accccacacc
     7321 cggcgtggaa tgccgtgtct tggcgatgtt cgggtacacc ttgaagcccg gaagttgacc
     7381 gccttcaccg ggtttggcgc cctgagccat cttgatctgg atgtcggtgg cattgacaag
     7441 gtagtcgctg gtaacgccga accgtcccga cgcgacctgc ttcaccgcgc tgcgcgttcg
     7501 tgggtcatag aggcgatcca cgtcctcgcc accctcaccc gagttggacc ggcccccgat
     7561 ggtgttcatc gcgatggcca tggtttcgtg ggcttcggcc gagatcgacc cgtagctcat
     7621 ggcgccggtg ttgaaccggc tgcagatcga ctcgaccgac tccacctcct ccaacggaac
     7681 gggcggcctt tcaccccgct tgaattcgaa caggccgcgc agcgtgccgc cttcgcggga
     7741 cagccggtcg acctcgtcgc tgtactgacg gaagatgtcc tcgcggcccg tgcgcgtcga
     7801 atgttgaagc aggaaaaccg tttcgggggt aaacaggtgc agctcacctt cccgccggaa
     7861 cgcatactca ccgccgacct cgagccggcg gtgcacccgt tcggtcgggt tgtccgggta
     7921 ggcccgacga tgacgctgcc tgacctcctc ggcgatcaca tcgagaccga caccgccgag
     7981 cttgctgggt gtgccggtga ggtactcgtc gacaaccgtc atgtcgaggc cgatcgcctc
     8041 gaaagcctgg gcgccggtgt aggagccgac tgtggagatg cccatcttgc tcatcacctt
     8101 catcacaccc ttacagagtg cggtcaggta gttgcgaacc gcggcaacgg tttcgatgcc
     8161 cgtcagctcg ccctcccgga tcaggtcctc gatggattcg aaggccagat acgggttgac
     8221 cgctgctgcg ccgtagccga tcagcaaagc gatgtgatgg acctcgcgcg cgtcgccgct
     8281 ttcgacgacg agggcaaccg atgtgcgctg attggtgcgg accaggtggt ggtggaccgc
     8341 cgcaacagcc agcagcgacg gtatcggcgc ccgggtgtgg tcggaatcgc gatcggagat
     8401 gacaagcgtg cgggcaccgt ccgcgatggc ttcacaggcg cggttcccga gatcgtcgat
     8461 cgcgccggcg agtccgtcgc cgccccgttc gacgtcgtaa agagcgcgca gcaccgtggc
     8521 tttccggccc gggtggtcgc cgtcgtcgtt gatgtgcacg atcttgttga gttcgtcgtt
     8581 gtcgagcacc ggccagcgca gcaggatctg gcgacacgac gccgcggacg gctcgaggag
     8641 gttctgctcg ggacccatga cgcgggcaag ggaggtgacg atctcctccc ggatggcgtc
     8701 cagtggcggg ttggtcacct gcgcgaacaa ctccacgaag tagtcgtaga agagcttggg
     8761 gcgttgcgag agaacggcgg gcggggtgtc ggtgcccatc gagccgagcg gttcggtccc
     8821 ggacgcggcc atcggggtga gcagaattct caggtcctct tcggtgtacc caaaggccac
     8881 ctggcggtgc gcaatcgact cgtggttggg ctgcacgcga gtgcgatcgg gtagcgcgct
     8941 caggtcgagc aaccccgcgt gcagccactc gccgtacgga tatgtctgtg ccagttcacg
     9001 tttgatttcg tcgtcgctga cgatgcgccc ggcggcggtg tcgaccagaa acatcctgcc
     9061 gggctccagc cgacccttcg cgacgacttc ggcagacggt atgtcgagca caccgctttc
     9121 gctggcgagt atcacgcgat cgtcgagggt gcgccaccac cgccctggcc gtaatccgtt
     9181 gcggtccaac actgctccga cgaccgtccc gtcggtgaag gtgaggcatg caggaccgtc
     9241 ccagggctcc atgatcgatg cgtggaactt ccagaagtcg cgaagtgccg gatccatcgt
     9301 ggtgttgttc tcccacgctt cgggaatcat catcatcacc gcgtgcggca ggccgcgacc
     9361 accgaggtgc agcagttcga ggacctggtc gaaggacgcc gaatcggacg actccggtgg
     9421 gcatatcggc gaaagccggc tcaggtcgcc gggaatcatc gcgctgttga gcatcgcctc
     9481 gcgggcgtgc atgcggttgc ggttaccgcg gacggtgttg atttcaccgt tgtgggcgat
     9541 gaagcggaat gggtgggcca acggccacga cgggaacgtg ttggtggaaa acctgctgtg
     9601 cacgatcgcg attgcggtca tgcatcgctg gtcgcgaaga tcgggaaagt actgcggcaa
     9661 ctggacagtg gtgagcatgc ctttgtagga gatggtgcgg ctggacagcg acggaaagta
     9721 cacaccggtg cgatccgcgc gcttgcgcag cggatacgcc cgccggtcga gatcgatgcc
     9781 gccgggtcgg atgccgtcgt gttcgggcgc ggtcacgaac aactgggaca tgtacggcat
     9841 gcacttcagc gccgtcatcc cgacatcggc tccgtcggga tccaccggaa ggtctcgcca
     9901 gccgcggatt tcgaggcctt cgtcggcggc gatgctttcg acgcagcttt gcgccttcgc
     9961 gcgcgccacc ggatcctgcg gcaggaagca caggccagca gcgtaggtgt tgcacccgtc
    10021 gagcgtgggc ggcggcagct cgaattccac tacctcttgg aacaattcga ccggcagctg
    10081 cagaagcacg ccggcgccgt cgccgctgtt ggtctcggcg cctgcggctc cccggtggtc
    10141 gaggtgctcg agggccaaca gaccgtcggc gagaatggca tgagaccgtc ggccgcggat
    10201 gtcggcgacg accgcgacac cgcatgaatc atgttcgtgc gcagggtcgt agaggccctg
    10261 cggctcgggc aacgccgaga acagcatgtg cacacctccg cagtcccgat tgtcgagtgg
    10321 gactgcgtcg gcccagattt ggggacgcgc attgccggcg cgccgaacct cgagtgtatc
    10381 agcggcgcgg ggatactacc cgctgtagag ggtcgggacc agcgggaaac ccggcatgtt
    10441 ccgaatcaca gtccatgcaa tggcggttgt gatgatcacg accacggccg ctcgcgtcag
    10501 cgccggcctt ccggtgtggc ggcgccagag gaaccacccg agcagagccg ggagtccgac
    10561 gagcaggacg atattgtcga cgacggctgc cgacaggttg gcgtgtagca ggtcgtgggt
    10621 cattcgcagg ccaccgcaag ccgggcagtt ccagccggtc agcagcttga aggggcacgg
    10681 cggaaagagg gcgccggggc ggtgtgggtc gttgatgccg acatatgcca cagccccagc
    10741 gagcaccgcg ccagtcgcaa gcgcggtgta ggccttgccc cgctggctgc gagcggaatc
    10801 aagtgccatc gcgcaaaggg cggccctggg ggtcgcggac gttgtcggtc agtatcagga
    10861 tggcgtcgat gacgccccag atccacgcac caatgaagca ggtgaagatg ccgacgagca
    10921 gctgagcgac gccgaggccc acctcaccca gatacattcg accgacaccc acgatgccga
    10981 atagcccaag cagctgcaga agaccggcaa cgagcttcga cttgtcggag
//
LOCUS       DASWBF010000374         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1223783,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000374 DASWBF010000000
VERSION     DASWBF010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223783"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..223)
                     /locus_tag="VF874_13935"
                     /pseudo
     CDS             complement(1..223)
                     /locus_tag="VF874_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739868.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding monooxygenase"
     gene            complement(467..1672)
                     /locus_tag="VF874_13940"
     CDS             complement(467..1672)
                     /locus_tag="VF874_13940"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9177122.1"
                     /translation="MSLSDNQDPIREAVDAGLLSGAVTMVWQRGEVLQVNEFGYRDVE
                     ARQPMTRDTVFRIASMSKPVTSAAAMTLLDEGKLALDDPVTRWLPEFEDMRVLDDRGG
                     PLEATSPARRPITVDDLLTHRSGLAYGFSVVGPLSRAYWHLPVRQQPDTWLAELTALP
                     LVHQPGERMTYSHATDVLGILLSRIEGKEFHKVLAERIFEPLGMADTGFFVTTEGRRR
                     AATMYKLDENDQLCHDVMGPPPIAPPAFCHGGGGLWSTVDDYLQFARMLLAGGEVDGT
                     RVLSAEAVRLMRTNRLTDEQRRQPFLGAPYWVGRGFGLGLSVVMDPEQSKPLFGHGGL
                     GTFGWPGAYGTWWQADPTLDLILIYLVQDHPSLSVDAAAAVAGNASQVTLMTARPRFV
                     SRTYRALEN"
     gene            1778..2341
                     /gene="pyrR"
                     /locus_tag="VF874_13945"
     CDS             1778..2341
                     /gene="pyrR"
                     /locus_tag="VF874_13945"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX9177123.1"
                     /translation="MGPINDKATDRELMSAADVGRTVSRIAHQIIEKTALDGRDAPHV
                     VLLGIPTRGVTLAERLAAKISDFAGVTVPHGALDITLYRDDLMMKPPRPLEDTSIPDT
                     GIDDALVILVDDVLYSGRSVRSALDALRDLGRPRAVQLAVLVDRGHRELPVRADYVGK
                     NVPTSRSESVHVLLTEDDGRDGVVITK"
     gene            2338..>2502
                     /locus_tag="VF874_13950"
     CDS             2338..>2502
                     /locus_tag="VF874_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX9177124.1"
                     /translation="MTPRHLLSAADLSRDDAVAILDDADRFAQALVGRDVKKLPTLRG
                     RTIITMFYENS"
BASE COUNT          414 a          868 c          827 g          393 t
ORIGIN      
        1 gaagccgcgc tgctccagcg agcgttcgac cctctgatcc atcagaccga tctgctcgac
       61 gatgccccgc cctgcgccgc gcaggtcgac cgtctgaacc gccaggccgg atcccgggag
      121 cgagttcgac gaagacgacg cgatagccgt tcgccgacaa ccagtgcgct agagcgggtc
      181 cggcgattcc ggcgccgctg atcaccacgg tggggcgggt catgcgactc agcgtagagg
      241 ctggcctatt ccgttcgtcg gcatcgtcca tcggagccca tttgcatcag ttacaccacc
      301 ggcccttcgt gggatggtgc gattcgtgcc cgccttgaag cggcaaatca atctgtcgct
      361 cagagccggg caattcgaac tgcaacccag gccagtgaca cgtgtggccg atgtgacgaa
      421 gtatcttcgc gggccaatag gatcagcggc tcgcgtccat catgtctcag ttctccagtg
      481 cccgataagt gcgcgagacg aatctcgggc gcgcggtcat cagcgtcacc tgcgacgcgt
      541 taccggccac cgccgcggca gcgtccaccg acagcgacgg gtggtcctgg acgaggtaga
      601 tcaggatcag atccagcgtc ggatccgctt gccaccacgt gccgtatgcg ccgggccagc
      661 cgaacgtgcc gagcccgcca tggccgaaca gcggcttgga ttgctccggg tccatcacca
      721 ccgagagccc cagcccgaac ccacgaccca cccagtacgg cgcaccgagg aacggctgcc
      781 gtcgctgctc gtcggtcagt cggttggtgc gcatcagccg caccgcctcg gccgacagca
      841 cgcgcgtccc atcgacttcg ccgccggcca gcagcatcct cgcgaactga agatagtcgt
      901 cgacggtgga ccacagaccg ccgccgccgt ggcagaatgc cggcggggcg atgggcggcg
      961 gccccatcac gtcgtggcat agctgatcgt tctcgtcgag cttgtacatc gtggccgcgc
     1021 gccgccggcc ttccgtggtg acgaagaacc cggtgtcagc catgcccagc ggctcgaaga
     1081 tccgctcggc cagcaccttg tggaactcct tgccctcgat ccgcgacagc aggatgccga
     1141 gcacgtcggt ggcgtggctg taggtcatcc gctcaccggg ctggtgcacc agcggcaacg
     1201 ccgtcagctc cgccagccag gtgtcgggct gctggcgcac cggaaggtgc cagtaggccc
     1261 gggaaagcgg gcccacgacg gagaatccgt aggccagccc gctgcggtgc gtgagcaggt
     1321 cgtccacggt gatcggccgg cgcgccgggc tggtcgcctc cagcgggccg ccgcggtcgt
     1381 cgagaacccg catgtcttcg aactcgggca gccacctcgt gaccgggtcg tcgagcgcca
     1441 gcttgccctc gtcgagcagg gtcatcgcgg cggcgctggt gacgggcttg ctcatcgacg
     1501 cgatccggaa gacggtgtcg cgggtcatcg gctgccgcgc ctccacgtcg cggtagccga
     1561 actcgttgac ctgcagcact tctccgcgct gccagaccat cgtcacggca cccgagagca
     1621 gtccggcgtc gacggcctcg cgaatggggt cctggttgtc ggacagggac agcgttgtca
     1681 tcgaccgtcc accctaacgg gcagtggtag gctcgccgca gtttggcatc ctttaacgat
     1741 ccgtcccgcg aggcggagaa ggaggtccag gtcgcaggtg ggcccgatta atgacaaggc
     1801 cactgaccgg gaattgatgt ccgcggcgga cgtcggccgc accgtttccc gcatcgccca
     1861 ccagatcatc gaaaagaccg ctctcgacgg gcgggatgcc ccgcacgtcg tgctgctcgg
     1921 gatcccgacc cgcggcgtga cgctcgccga gcggctcgcg gccaagatca gtgacttcgc
     1981 cggcgtcacg gtcccgcacg gggcgctgga catcacgctc taccgtgacg atctgatgat
     2041 gaaaccgcca cggccactgg aggatacgtc gataccagat accggcatcg atgacgcgct
     2101 ggtgatcctg gtcgacgacg tgctgtactc cggccggtca gtgcgctcgg ccctcgacgc
     2161 gctgcgcgac ctcggccggc cccgggcggt gcagctggcg gtgctcgtcg accgcggtca
     2221 ccgcgagctg ccggtgcgcg ccgactacgt cggcaagaac gtcccgacgt cgcgaagtga
     2281 gagcgtgcac gtgctgctga ccgaggacga cggccgcgat ggggtggtga tcaccaaatg
     2341 acgccccggc acctgctttc cgcggccgac ctgagccgcg acgacgccgt cgcgatcctc
     2401 gacgacgccg accggttcgc gcaggcgctg gtgggccgcg acgtcaagaa gctgccgacg
     2461 ctgcgcggcc ggaccatcat cacgatgttc tacgagaact cc
//
LOCUS       DASWBF010000375         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_694732,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000375 DASWBF010000000
VERSION     DASWBF010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694732"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1382
                     /locus_tag="VF874_13955"
     CDS             180..1382
                     /locus_tag="VF874_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX9177125.1"
                     /translation="MSLAVERQLTEKYESIASLLDERQRRRWLGIEARALGRGGVSAV
                     ARATGASRTTVTAAVAELAGGGDDSAGRVRRAGAGRPPVSQRDPAVVAALERLVDPTT
                     RGDPESPLRWTSKSTRTLADELCAQGHSVAPRTVAKLLHESGYSLQATRKTREGRDHP
                     DRDAQFRYLSGQINAHLGSGDPVVSVDTKKKELVGRYKNAGREWQPTGQPEQVEVYDF
                     IGEAGKAIPYGVYDVAANAAWVSVGRDHDTAAFAVATLRRWWQAMGRPLYPRSRRLLI
                     CADGGGSNGYRVRLWKVELAALAAETGLEITVCHLPPGTSKWNKIEHRLFSHISMNWR
                     GRPLESHETVVQLIAATTTHSGLAVQAELDDRTYPKGTKITDEQMASLPLTRHDFHGE
                     WNYTLQPE"
     gene            complement(1409..2275)
                     /locus_tag="VF874_13960"
     CDS             complement(1409..2275)
                     /locus_tag="VF874_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9177126.1"
                     /translation="MVVGVDGSPPSKVAVDWAARDAAMRKIPLTVVHVLNPPTMVMTF
                     PDTPMPPSPQYMQWQEDEERTILNDALKTVEESTKRVGRIDVSGELMTGSAVPTLVEL
                     SKDAEMVVVGCRGRGALARSLLGSVSTGLVHHAHCPVAVIHDENPLMPHPSQAPVLVG
                     IDWSPASESATAIAFYEASRRRVELVALHVWSDNALSQSVGFSRMQSLSEETLAKLLA
                     GWQERYPDVKVRRIIVCDRPAHQLVEQSESAQLVVVGSHGRGGFTGMLLGSVSTAVVH
                     SARVPVIVARRP"
BASE COUNT          424 a          747 c          753 g          362 t
ORIGIN      
        1 cagctgatca cacagatgcg caaaatcagt gaccgtgcaa ccgaactcat cctggcagcc
       61 gacgcccgag cagtcgactg accagatcac agggtcaacg cagacggagg ttaaaaatca
      121 agctaaggac acgcgcataa attaatcgaa cgatgggtgc agctggctag gctcctcgtg
      181 tgtctttggc ggtggagcgg cagttgactg agaagtacga gtcgatcgct tcgcttctcg
      241 atgagcggca gcggcgtcgt tggctcggga tcgaggcgcg ggcactgggc cgtggcggag
      301 tgtcggcggt agctcgtgcg accggggcgt cgcgcacgac ggttactgcc gcggtggcgg
      361 aactggccgg cgggggcgat gattcggcgg ggcgggtgcg ccgagcggga gcgggtcgcc
      421 cgccggtgag ccagcgtgat cccgcggtgg tggcggcatt ggagcgtctg gtagacccga
      481 ccacccgcgg cgatcccgag tcgccgttgc ggtggacgtc gaaatcgacc cggacgttgg
      541 cggatgagct ttgtgcccaa gggcattcag ttgcgccgcg gacggtggcc aagctgctgc
      601 acgagtccgg ctacagcctg caggccaccc gcaagacccg cgaaggccgt gatcacccgg
      661 accgggatgc gcagttccgg tatctcagcg ggcagatcaa cgcgcatctg ggcagtgggg
      721 atccggtggt gtcggtggac accaagaaaa aggaactcgt cggccggtac aaaaacgcag
      781 gccgcgaatg gcagcccacg ggtcagccgg agcaggtcga ggtgtacgac ttcatcggtg
      841 aagcgggcaa ggccatcccc tacggggtct acgacgtggc cgccaacgcc gcctgggtgt
      901 cggtcggccg tgaccacgac accgccgcct tcgcggtcgc caccctacgg cgctggtggc
      961 aggcgatggg tcggccgctc taccctcgct cccgccggct gctgatctgc gccgacggcg
     1021 gcgggtccaa cggataccgg gtgcggctat ggaaggtcga actcgccgcg ttggccgccg
     1081 aaaccgggct cgagatcacc gtctgccacc tgccgcccgg caccagcaaa tggaacaaaa
     1141 tcgagcaccg gctgttcagc cacatctcga tgaactggcg cggccggccg ctggaaagcc
     1201 acgagaccgt cgtccagctc atcgctgcca ccaccaccca cagcggactc gccgttcagg
     1261 ccgaactcga cgaccgcacc taccccaaag gcaccaagat caccgacgag cagatggcct
     1321 cactgcccct gacccgccac gacttccacg gcgaatggaa ctacaccctc caacccgaat
     1381 aacgtccatg taatttatgc gcaagtcctt aaggtcgacg ggcgacgatc accggcaccc
     1441 gggcggagtg gaccaccgcg gtgctgaccg aacccagcag catcccggtg aacccgccgc
     1501 ggccgtggct gccgacgacg accagctgcg cggactcgga ctgctcgacc agttgatgcg
     1561 ccggccgatc gcacacgata atgcggcgta ccttgacgtc aggatagcgt tcctgccatc
     1621 cggcaagcag cttagccagg gtttcctcgc tcagtgactg cattctcgaa aagccgacgc
     1681 tctgcgacag tgcgttgtca ctccagacgt gcagggcgac cagttccacc ctgcggcgcg
     1741 acgcttcgta gaaggcgatt gcggtggccg actccgatgc gggtgaccag tcgataccga
     1801 ccagcaccgg tgcctgcgac ggatggggca tcagtgggtt ttcgtcatgg atgactgcaa
     1861 ccgggcagtg cgcgtgatgg accagcccgg tgctgaccga cccgagcagg cttcgggcca
     1921 gtgcgccgcg gccacggcac cccaccacga ccatctcggc gtccttggac agctcgacaa
     1981 gggtcggaac cgccgatccg gtcatcaact caccgctgac gtcgatccga ccgacccgct
     2041 tcgtgctttc ctcgacggtc ttgagcgcgt cgttgagtat cgtgcgttcc tcgtcctcct
     2101 gccactgcat gtactggggt gacggcggca tcggggtgtc ggggaacgtc atcaccatcg
     2161 tcggcgggtt caatacgtgg accaccgtga gggggatctt gcgcatggcc gcgtcgcgtg
     2221 ccgcccagtc aacggcgacc ttcgagggcg gcgacccatc gacaccgacg acgattccac
     2281 gatgcg
//
LOCUS       DASWBF010000376         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_245726,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000376 DASWBF010000000
VERSION     DASWBF010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245726"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF874_13965"
     CDS             complement(<1..213)
                     /locus_tag="VF874_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX9177127.1"
                     /translation="MAPRACPHLGADLATAAVECGTLVCPWHRLRLSDQSRGAWQPVS
                     GPRRRCRLDAVGKEEPTDAPIIPARPP"
     gene            complement(478..1992)
                     /locus_tag="VF874_13970"
     CDS             complement(478..1992)
                     /locus_tag="VF874_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9177128.1"
                     /translation="MRQTLPAPAGRSDAGSLARVPRVVVVGAGIAGLAAATGLSERGV
                     SVDVVEARDYLGGRVGGWPDTLTDGSAIAMNRGFHAFFRQYYNLRALLRRADPHLSAL
                     SPVDDYPLIDAFGRTDTFRGLPRTPPLNALAFALRSPTFRLRDLVRLDARAAAPLATV
                     SVPNTYGELDDIDAGTFLQDINFPDAARHLAFEVFARSFFARPENLSAAELVTMFHIY
                     FLGSSEGLLFDVANANFDAALWNPLQKYLESRGVRFHTGLAVDGLTMASDLRVTCGTD
                     AGFTADAVVLATDVAGLRRIVAGSPGLGDDDWRHRVGELHTAPPFIVLRLWLDRPVRA
                     DRAPFMGTGGRPPLDNVSVLERYEREAAQWAARTGGSVIELHAYSVIDPDASVRDALV
                     ARMHELYPETAGASIVDERLLRRDDCPRFAPGDFARRPGVQTPESRVVLAGDGIRIDL
                     PVALMERAATTGWVAANHLLGHFGLAGHAVHTVPNRGRSALLRRLAARGGNQVR"
     gene            complement(2007..2336)
                     /locus_tag="VF874_13975"
     CDS             complement(2007..2336)
                     /locus_tag="VF874_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX9177129.1"
                     /translation="MSGLGYTLAAVLAVIAVCVLEMRVLRTRLFRRPAYWISMVIVLG
                     FQIPVDGWLTKLSAPIVLYNEQHTTGVRFPFDIPIEDFLFGFALVTAVLLLWERRRPQ
                     IEMADQR"
     gene            complement(2333..2668)
                     /locus_tag="VF874_13980"
     CDS             complement(2333..2668)
                     /locus_tag="VF874_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX9177130.1"
                     /translation="MWTAGSVLGACLLITAPLELFGRGVYRQPRRLLGAALPVAVVFL
                     IWDAIAVAADVWWYNPRYILGLIAPGDLPLEEILFFVVIPLCGLLTYSAVDDILFMVS
                     RRRAGARAR"
     gene            complement(2669..>2858)
                     /locus_tag="VF874_13985"
     CDS             complement(2669..>2858)
                     /locus_tag="VF874_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX9177131.1"
                     /translation="APASRPCVAVAWTLYSEILKRIEEDDFQIFGQRATVGKARRLTV
                     AGGGLLRSWTARLRHGSR"
BASE COUNT          538 a          968 c          922 g          430 t
ORIGIN      
        1 cggcgggcgg gccgggatga tcggagcgtc ggtgggctcc tctttgccga cggcgtcgag
       61 gcgacaccgt cgtcgtgggc cggaaaccgg ttgccatgca cctctactct ggtccgagag
      121 ccgcagacgg tgccacgggc acaccagcgt tccgcattcg accgcggcag tggccaggtc
      181 cgcccccaga tgcggacacg cgcgaggcgc cacccgtagc gctccatcgg ctccgcgcca
      241 ggccacgatc tcgaccccca cccacagtgg tgccccaggc cttggtcgcg atctgcgagc
      301 tcgctgcgaa cgcgaaccag ttgccgctcg ggcggcactg cgcacgcgcg accgccgccc
      361 ggatcacccc cggatcggct tcgcggtacg tgggcccttg cgccgaccag tcgagcgtcg
      421 gaagtgtctg gaaaggccac gctttgaccg ctctgtgatg cagatcggac agcttgctca
      481 tcgaacttgg tttcctccgc gcgccgccag ccgacgcagc agcgccgatc gaccgcgatt
      541 cggaacagtg tgcacggcgt gtccggccag accgaagtgg ccgaggagat ggttggccgc
      601 aacccatccg gttgtcgcgg cgcgctccat caacgccacc ggcaggtcga tacgaattcc
      661 atcccccgcc aacaccactc gtgattccgg tgtctgcacc ccgggccgcc gggcgaaatc
      721 tcccggcgcg aaccgcgggc agtcatcgcg gcgtaacagt cgttcgtcga cgatcgacgc
      781 gccggcggtt tcggggtaga gctcgtgcat ccgtgccacc agggcgtcgc gcaccgaggc
      841 gtcggggtcg atcaccgagt aggcgtgcag ttctatcacc gatccgccgg ttcgggcggc
      901 ccactgtgct gcttcgcgtt cgtagcgctc caagacgctg acattgtcca acggtggacg
      961 accacccgtg cccatgaagg gggcgcgatc ggcacgtacc ggacggtcca gccacagacg
     1021 cagcacgatg aagggtggcg ccgtgtgtag ctcgccgacc cggtggcgcc agtcgtcgtc
     1081 accgagaccg ggcgatcctg caacgatgcg gcgcaatccg gcgacatcgg tggccagaac
     1141 gaccgcatcg gcggtgaagc cggcatcggt tccgcatgtc acccgcagat cggaggccat
     1201 ggtcaggcca tcgactgcca gcccggtatg aaaacgcaca cctcgggatt ccagatactt
     1261 ctgcagcgga ttccacagtg cggcatcgaa gttcgcattc gcgacgtcga acagcaggcc
     1321 ttcgctcgac ccgaggaagt agatgtggaa catcgtcacc agctcggctg ccgacaggtt
     1381 ctccggccgg gcgaagaaac tgcgggcgaa cacctcgaag gccagatgcc ttgccgcatc
     1441 gggaaagttg atgtcctgaa ggaacgtgcc ggcatctatg tcgtcgagtt caccgtaggt
     1501 gttgggcacc gataccgtgg ccaacggcgc ggcggctctg gcgtcgagcc gaactagatc
     1561 gcgcagccgg aatgtcggac tgcgcagcgc gaaggccagt gcgttcagcg gtggggtgcg
     1621 cggcaacccg cggaatgtat cggtgcgtcc gaacgcatcg atcaacggat agtcatcgac
     1681 cgggctcaac gctgacaggt gcggatcagc gcggcgcagc aaggctctca ggttgtagta
     1741 ctggcggaag aacgcgtgga agccccggtt catggcgatg gccgaaccgt cggtcagggt
     1801 gtcgggccag ccgccgactc ggccgccgag gtagtccctt gcttcgacga catcaacgct
     1861 cacgccccgc tcgctgagtc cagtcgcagc cgcaagaccg gcgatgcctg cgccgacgac
     1921 gacgacgcgg ggcacccgag caagtgagcc ggcatcgctg cgacccgccg gagccggcag
     1981 cgtctggcgc atccgatctg tctgactcat cgctgatctg ccatctcgat ctgcggccgg
     2041 cgccgctccc agagcaacaa cactgcggtc accaacgcga agccgaacag gaaatcctcg
     2101 atcggtatgt cgaacggaaa ccgaaccccg gtggtgtgct gttcgttgta aaggacaatg
     2161 ggcgcactga gtttggtcaa ccagccgtcg actgggatct ggaagcccag cacgatgacc
     2221 atggagatcc agtacgcggg tcggcggaac agcctggtgc gcagcacccg catttccagc
     2281 acacaaaccg caatcaccgc aagcaccgcg gcaagcgtgt agcccagacc actcatcgcg
     2341 ccctggcccc cgcgcgccga cggctgacca taaagaggat gtcatcgacc gccgagtagg
     2401 tcagcaaccc gcacagcggt atcaccacga agaacaggat ttcctccaac gggagatcac
     2461 cgggtgcgat caggcccagg atatagcgcg ggttgtacca ccagacgtcg gcggcaaccg
     2521 cgatggcgtc ccagatgagg aacaccacgg ccacgggaag cgcagcgccg agcaaccgcc
     2581 tcggctgccg atacacacca cggccgaaca gctcgagcgg agctgtgatc agaaggcatg
     2641 cgcccaggac gctgccagct gtccacactc acctactccc gtgtcgcagt cgcgcagtcc
     2701 acgaccgtag gagaccgccc ccggcgacgg tgagtcgccg ggctttccct acggtggccc
     2761 gctggccgaa gatctggaag tcgtcttcct cgatgcgctt gaggatctcc gagtacagcg
     2821 tccacgccac tgcgacgcac ggacgcgacg ccggggcc
//
LOCUS       DASWBF010000377        18590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_509805,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000377 DASWBF010000000
VERSION     DASWBF010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18590
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509805"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VF874_13990"
     CDS             <1..145
                     /locus_tag="VF874_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006899392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9177132.1"
                     /translation="VDDAAIVGDTDYVRKQIAVWEAAGVTTMVVTPRSADEITHLGEL
                     VLH"
     gene            151..867
                     /locus_tag="VF874_13995"
     CDS             151..867
                     /locus_tag="VF874_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX9177133.1"
                     /translation="MPASQHTIAGTVLTMPVKIRKANQHMAMFSVDADAAQRMIDYSG
                     LQVAHYLPGRAAVALILAHFIDGDLGEYHEFSTSVMVNPPGSKVSGFRALQSAFIHHM
                     LVDQTFTLEAGRTIWGFPKVMADFTVGDAGQFGFDVSIDGQLVADMEFRRGLPVPSAL
                     TYRQQELRSYSHRDGLTLETPFEMSTVGSRYRPGGVRLRLGDHPYAKELASLGLPKRA
                     LLSTSAQNVEMSFGDAQNVR"
     gene            880..2079
                     /locus_tag="VF874_14000"
     CDS             880..2079
                     /locus_tag="VF874_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9177134.1"
                     /translation="MTTTTKPDVDLTDGAFYAGDSRSIYKWMRENEPVFRDRNGLAAA
                     ATYQAVIDAERQPELFSNAGGIRPDQDAPPMMIAMDDPAHLLRRKLVNAGFTRKRVKD
                     REAAIGTLCDTLIDAVCERGECDFVWDIAAPLPMAVIGDMLGVLPQEREMFLKWSDDM
                     VSFLSSTTAQEDFQVSMDAFAVYTDYMMRMIADRKKEPTDDLVSVLVHAEVDGEQLAD
                     HEIVTEVLLLLIGGDETTRHTLSGGTAQLLRHPDQHARLVNDLALLPNAIEEMLRWTA
                     PVKNMARTLTADTEFRGTELKQGEKMILLFESANFDEKVFGDPENFRIDRNPNNHLAF
                     GFGTHFCLGNQLARLELSLMQTRLLQRLPDMRLVSEDALPLRPANFVSGLERMPVRFT
                     PTKPLTG"
     gene            2270..3619
                     /locus_tag="VF874_14005"
     CDS             2270..3619
                     /locus_tag="VF874_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9177135.1"
                     /translation="MGTDRVHYARNGDVRLAYRVFGDADITLVWVPGWVSNVDLYDDP
                     TTPFAPVVAQLARKTRLVVWDKRGTGLSDPVTRIPPLDERMDDLHAVLDAVDADRPAL
                     LGVSEGGPMSVLFAATYPERVRSMVLYGTAARFVREPPDFPWGVPPDRIEAGVASIDE
                     HWGEGYLAERILGDAVEVPGVREMLGRYERASASPTIAGMLWRSLAEIDIREILKTVR
                     TPTLVLSRPGDQMVPIESSQALAAGIPGAVFRELPPGAHAALDIDDSLAAETLAFVCG
                     ETETAVNERVLTTVLFTDIVGSTEQVVAAGDAHWRHRLDTHDELVDRLLSKYGGKRAK
                     HTGDGIFALFDGPTKAARCALDLVPALATRGIRIRAGVHIGECERRGEEWSGLTVHTG
                     ARIGAMAGPGEVLTSRTVRDLSAGSGLRFQSLGGHRLKGIPEATEVFRVTAPAGASF"
     gene            complement(3713..4513)
                     /locus_tag="VF874_14010"
     CDS             complement(3713..4513)
                     /locus_tag="VF874_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003916172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9177136.1"
                     /translation="MSEFDKGADALIEQRGHTLILTLNRPEARNALSTEMLSIMVQAW
                     DRVDSDPDIRSCILTGAGGYFCAGMDLKAATARPPGDSFKDGSYDPSRIDALLKGRRL
                     TKPLIAAVEGPAIAGGTEILEGTDIRVAGESAKFGVSEAKWSLYPMGGSAVRLPRQIP
                     YTLAADMLLTGRHLSAAEAKEIGLIGYVVPDGQALTKALEIAETVNNNGPLAVQAILK
                     SIRETEGMPENEAFKIDTQLGIQVFLSDDAKEGPRAFAEKRKPEFKNR"
     gene            4563..6212
                     /locus_tag="VF874_14015"
     CDS             4563..6212
                     /locus_tag="VF874_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9177137.1"
                     /translation="MGSVALNIADLAEHAIDAVPDRAALICGDEQLTYAELEEKANRL
                     AHYLIDQGVKKDDKVGLYCRNRIEIVIAMLGIVKAGAILVNVNFRYVEGELRYLFDNS
                     DMVALVHERRYADRVANVLPDTPNVKTVLVVEDGTDLDYQRYGGVEFYEALAQGSPER
                     NFGERSRDDIYLLYTGGTTGFPKGVMWRHEDIYRVLLGGTDFATGEYVKDEYDLSKQA
                     AAGPPMIRFPIPPMIHGATQSATWMALFSGGTVVLAPEFDADEVWRTINEHKVNLLFF
                     TGDAMARPLLDALSKDNDYDLSSLFLLASTAALFSPSIKEKLLELLPNRVITDSIGSS
                     ETGFGGTSLVAKGETNTGGPRVTIDKWTVVLDEDGNEVKPGSGVRGIIAKRGHVPVGY
                     YKDPEKSAATFKVINGVRYAIPGDYAQVEADGTVTMLGRGSVSINSGGEKIYPEEVEA
                     ALKGHPDVFDALVVGIPDERYGQCVAAVVQPRKGTRPSLSDLDAFVRSEIAGYKVPRA
                     LWYTDEVKRSPAGKPDYRWAKEQTEGRPADEVSTKHAGAKT"
     gene            6212..6541
                     /locus_tag="VF874_14020"
     CDS             6212..6541
                     /locus_tag="VF874_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177138.1"
                     /translation="MRTELCDRFGIDYPIFVFTPSEKVAAAVSKAGGLGVLGCVRFNE
                     ADDLEDVLHWIPPSTSTSLFRSNIGSSSTKPLPTLGFHRCRKMRIETKVFWAGFIRSP
                     GQMSRWR"
     gene            6532..6672
                     /locus_tag="VF874_14025"
                     /pseudo
     CDS             6532..6672
                     /locus_tag="VF874_14025"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
     gene            7263..7499
                     /locus_tag="VF874_14030"
     CDS             7263..7499
                     /locus_tag="VF874_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177139.1"
                     /translation="MTAKTTTWIRRVAAGAVLVAAPAFIALDTAAASNAETSSTSPSY
                     DYSPTPSQTTQYPYAPWYQSFHQRHAAEVQSWYR"
     gene            complement(7523..9109)
                     /gene="fadD17"
                     /locus_tag="VF874_14035"
     CDS             complement(7523..9109)
                     /gene="fadD17"
                     /locus_tag="VF874_14035"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD17"
                     /protein_id="HEX9177140.1"
                     /translation="MNPASGSDLPTVTSLITPLVDVDDRGVYFADEPLARRGDNADEP
                     LARRGENQDSFVSWRNHIARGAAVAAALRKRLDPARPPHVGVLLQNTPFFSSLLIAAA
                     LSGIVPVGLNPIRRGAALDRDVRHADCQVVLADSALAAGLSDTDYIDVDSADWAAELQ
                     AYQDAPVVARSAAPTDPFMLIYTSGTSGDPKAVICSHGKVAIAGVTMTERFSLGPSDI
                     SYVSMPLFHSNAVLVGWSVALACRGSMVLRRKFSASQFIPDVRRYGATYANYVGTPLS
                     YVLATPERPDDADNPLKAVYGNEGAPGDIERFARRFGTVVVDGFGSTEGGVAIARTPD
                     TPPGSLGPLPDGVEIVDVDTGEPCPPGVTGELVNIGGAGRFEGYYNDPDADAERMAGG
                     VYHSGDLACRDVAGYVYFAGRLGDWMRVDGENLGAAPIERVLLRNADVVEVAVYPVPA
                     PDVGDQVMAALVLTEGVTFDPDAFRKFLVEQPDLGPKQWPSFVRISAELPRTQTFKVL
                     KRQLSAQGIECDDPVFPIPR"
     gene            complement(9106..10203)
                     /locus_tag="VF874_14040"
     CDS             complement(9106..10203)
                     /locus_tag="VF874_14040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177141.1"
                     /translation="MDFKTTEAATDLGGLVRTITESVCTPEHQRELDGLDERFDRDLW
                     RTLIDSDVLSAAAPESLGGGGFGVLEQVAVLVALGRQMAAVPYFDSAVLAAGALAKFG
                     SAALQQEWASPAVNGEKILAVALDGEMGEGPVQATASGSGYRLTGTRTQVAYGPVADA
                     FLVPSETDSGIKVFLVAAADAGVSVTSLQTTGHGSVGHLELQGVEVDSERVVGGEEVV
                     GWLVTHATLGRTAFQLGVLERALELTAEYAREREQFDRPIGSFQAVAQRLADGYIDVK
                     GLRLTTTQAAWRLSEDLPADLEVATAAFWAADAGHRVAHTTVHVHGGVGIDLDHPVHR
                     YFLAAKQTEFAVGGATGQLLRIGRELADTPA"
     gene            complement(10213..11394)
                     /locus_tag="VF874_14045"
     CDS             complement(10213..11394)
                     /locus_tag="VF874_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9177142.1"
                     /translation="MRIGYGPEQEELRRELRSYFGKLMTPERVEALSSVEGEYGTGNA
                     YRDTVAQMGKDGWLALGWPTEYGGQARSAMDQLIFTDEAAIAGAPVPFLTINSVAPTI
                     MAFGTDEQKKFYLPKIASGELHFSIGYSEPGAGTDLAALRTTAVRDGDEYVINGQKMW
                     TSLIPYADYVWLAVRTNPEAKKHRGISMLIVPTTAPGFSWTPVHTMAGPDTSATYYQD
                     VRVPVTNLVGEENGGWKLVTNQLNHERVALVSAQPIFLALNAVREWAQNTKDIHGSRL
                     IDSEWVQLNLARVHAKAEVLKLINWELASSDNASPSPADASAAKVFGTELATEAYRLL
                     MEVLGSAATLRPNSPGALLRGRVERMHRACLILTFGGGTNEIQRDIIGMVALGLPRVN
                     R"
     gene            11568..11759
                     /locus_tag="VF874_14050"
     CDS             11568..11759
                     /locus_tag="VF874_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9177143.1"
                     /translation="MRVEVDRDRCEGNAVCVGIAPDLFDLDDEDYAVVKKDPVPADEE
                     DLAEQAIAECPRAALLRKD"
     gene            11818..12708
                     /locus_tag="VF874_14055"
     CDS             11818..12708
                     /locus_tag="VF874_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEX9177144.1"
                     /translation="MTDLSGRVAVVTGAAAGLGRAEAIGLAAVGATVVVNDLDAALND
                     SDVFDEIKAAGSKAVAVAGDVGERSTADEMVAAAEREGGLDIVVNNAGITRDRMLFNM
                     SDEDWDLVIKVHLRGHFLLTRNAAAYWRSRAKESGGQTYGRLINTSSEAGLSGPVGQA
                     NYGAAKAGITALTLSAARALGRYGVCANAICPRARTAMTADVFGEAPDDADIDPLSPD
                     HVVTLVRFLASPAAEAVNGQVFIVYGPSVTLVAAPTAVHRFTAEGPAWAPQELSSAMQ
                     KFFADRDPDMTFAATQLMER"
     gene            12974..13738
                     /locus_tag="VF874_14060"
     CDS             12974..13738
                     /locus_tag="VF874_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9177145.1"
                     /translation="MKDQLAVPARAVGGFFEMAGDTFRAIFRRPFQFGEFLEQTWMIA
                     RVSLVPTLLVSIPFTVLVAFTLNILLREIGAADLSGAGTAFGTITQLGPVVTVLVVAG
                     AGATAICADLGARTIREEIDAMRVLGIDPIHRLVVPRVLASTLVALLLNGLVCAIGLS
                     GGYVFSVLLQGVNPGAFINGLTVLTGLGELMLAEVKALLFGVCAGLVGCYRGLTVKGG
                     PKGVGNAVNETVVYAFICLFVINVLMTAVGVRVLAR"
     gene            13735..14577
                     /locus_tag="VF874_14065"
     CDS             13735..14577
                     /locus_tag="VF874_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9177146.1"
                     /translation="MSYDVTLRLRRLFSGVPKVVDSFGEQALFYAESIRWIPNAVTHY
                     RKETVRLIAEMTLGTGALLVIGGTVGVAAFLTLASGGVIAVQGYSSLGNIGIEALTGF
                     LSAFLNVRIVAPVIAGIALAATIGAGTTAQLGAQRVAEEIDAFEAMAIHSVSYLVSTR
                     IVAGLIAIVPLYSLSVLAAFFAARFTTVYINGQSGGLYDHYFNTFLVPTDLLWSFLQA
                     IAMSIAVMLVHTYYGYNASGGPVGVGIAVGQAVRTSLIVVVVITLFISLAVYGASGNF
                     NLSG"
     gene            14587..15789
                     /locus_tag="VF874_14070"
     CDS             14587..15789
                     /locus_tag="VF874_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9177147.1"
                     /translation="MANSGSKTRHGRIAAAVLATLILAFAVLTYLSYTAAFTSTDSVT
                     VTSPRAGLVMEKDAKVKYRGIQIGKVKDIAYSGDQAKLTLAIDSNQMRYIDSNAEVRI
                     ASNTIFGAKSVEFVPPTKPSGTSLRPDARVQVSAVQLEVNTLFQTLEDLLHKINPIDL
                     NATLSAIAEGLRGHGDDFGASLAGLNELLPKINPKMPTLQSDFQQAAAVGNIYGDAAP
                     DLVTVLDNVPTISDTLVDEQENLNATLLAATGLADNGTATLEPAQEDLIAALQRFRAP
                     LKVLSDYSPEFGCIFTGVKKALDQFAPVIGGTRPGLFTSSNFVLGVPGYTYPESLPIV
                     NATGGPNCRGLPNIPTKQFGGSWFHSPFLVTDNAYIPYQPLTEVQVDAPSTLQFLFNG
                     AFAERDDW"
     gene            15853..16911
                     /locus_tag="VF874_14075"
     CDS             15853..16911
                     /locus_tag="VF874_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9177148.1"
                     /translation="MPGHRGVLIKVSIFTVVMLLVAAGLVVIFGQFRFSSTNTYHAIF
                     ENASRLKGGNDVRIAGIPVGSVKDIQLKPDNKVDVTFSVDKRYQMYTSTKAVIRYQNL
                     VGDRYLEITSGPGELRKVPPGGTIQHTEPALDLDALLGGLRPVLKGLSGDKVNEISSA
                     IIELLQGQGGALSDLLSSTGSFSRTLADRYQVISDVIHNLNTVLTTVDEKSAQFNESI
                     DQLQQLVTGLAQGRDPIAGALQPLASAASDLTDVLQSSRRPIQGVIENLRPLATEMDN
                     RRGEINAVVEPLAENYLRLNALGAYGAYFNIFYCSVRIKINGPSVGPPTGTMSDSDIL
                     IPQGGPPDPSKGRCSENG"
     gene            16904..17956
                     /locus_tag="VF874_14080"
     CDS             16904..17956
                     /locus_tag="VF874_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9177149.1"
                     /translation="MAEGRNPLRTGVIGLVLVVGVVLVSFGYNKLPFWPQGKTYEAYF
                     TDAGGISPGNEVNVSGLKVGQVSSVALAGDVAKVTFTVDRKIKVGDQSLVSIKTDTIL
                     GEKSLSVTPGGRGDSTTIPLNRTTAPYTLNNALQDLGQTASDLDKDKFTQALGVLTDS
                     LHEATPQLRQALDGVAALSRSINANDEALAQLLERARSVTKVLADRAGQVNRLIVDGN
                     QLFAALDERRAAISTLISSIEPVSQQIEGFVADNRREFGPALRKLNAVLDNLLQRREH
                     ISEALRRLPPYATALGEVVASGPGFHINLYGLPPGPLGEVLLDLYFQPGRLPASLADY
                     LRGFISERLIIRPKSP"
     gene            17980..>18590
                     /locus_tag="VF874_14085"
     CDS             17980..>18590
                     /locus_tag="VF874_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9177150.1"
                     /translation="MRWLRYGAITALVAAFIAGIYVIWPGRAGHKVIGYFTSAVGLYP
                     GDDVRIVGVPVGTIKSVEPQAQAVKITMSVKDGVKIPAGAQAVIMAPNIVSARFVQLT
                     PAYTSGPVMADGATIGLDRTAVPVEWDEVKSELTKLSASLGPQAGSMQGPLGDFVNQA
                     ADTFDGNGDSFRQALRELSQTTGRLGDSRTDLFGTIKNLQILI"
BASE COUNT         3300 a         6070 c         5976 g         3244 t
ORIGIN      
        1 cgtcgacgac gccgcgatcg tcggcgacac cgactacgtg cgtaagcaga tcgcggtctg
       61 ggaagccgca ggggtgacga cgatggtggt caccccgcgc agcgccgacg agataacgca
      121 cctcggcgag ctggtcctac attgaacgcc atgcctgcca gccaacacac catcgccggc
      181 acggtgctca ccatgcccgt gaagatccgc aaggcgaatc aacacatggc gatgttctcg
      241 gtggacgccg acgccgcgca acggatgatc gactacagcg gcctgcaggt ggcccactat
      301 ctgcccgggc gtgcggccgt cgcgctgata ctggcgcact tcattgacgg cgatctaggc
      361 gagtaccacg aattcagcac tagtgtgatg gtcaacccgc ccggatctaa ggtgtccggt
      421 tttcgtgcgc tgcagtcagc gttcattcac cacatgctgg tcgatcagac cttcactctg
      481 gaagcgggac ggacgatttg gggatttccc aaagtcatgg ccgacttcac ggttggtgat
      541 gcgggtcagt tcggcttcga cgtgagcatc gacggacagc tggtggccga catggaattc
      601 cgtcgcggcc tgccggttcc gtcggcgctc acctatcgcc agcaagagtt gcgctcgtat
      661 tcgcatcgcg acggtctcac gttggagact ccctttgaga tgtcgacggt gggttcgcgt
      721 tacaggccag gcggtgtgcg gctgcgactc ggtgaccatc cctatgcgaa agagcttgcg
      781 tcgctcggct taccgaagcg ggcgctgctg tcgacttctg ctcagaacgt cgagatgtcg
      841 ttcggtgacg cgcagaacgt gcgctgaagg gagctacttg tgacgacgac gaccaagccg
      901 gacgtagacc tcaccgacgg cgctttctat gccggcgact cgcggtccat atacaaatgg
      961 atgcgcgaaa acgagccggt attcagggat cgcaacggct tggccgccgc ggcgacttac
     1021 caggcggtga tcgacgccga gcggcagccc gaactgttct ccaacgcggg cggcatccgg
     1081 cccgaccagg atgcgccacc gatgatgatc gcgatggacg accccgcgca tctgttgcgg
     1141 cgcaagctcg tcaacgcagg cttcacacgc aagcgcgtaa aggacaggga agcggctatc
     1201 ggcactctgt gcgacacgtt gatcgacgcg gtgtgtgagc gcggggagtg cgacttcgtc
     1261 tgggacatcg ccgcgccgct gccgatggca gtcatcggcg acatgctcgg cgtgcttccg
     1321 caggagcgcg agatgttcct caagtggtcc gacgacatgg tgagcttctt gagcagcacc
     1381 acggcgcaag aggatttcca agtttcgatg gacgctttcg ctgtctacac cgactacatg
     1441 atgcgcatga tcgcggatcg caagaaagag ccgaccgacg atctcgtcag cgtgttggtg
     1501 catgcggagg tcgacgggga acagttggcc gaccacgaga tcgtcaccga ggtgctgttg
     1561 ctcttgatcg gtggcgacga gaccacccgg cacacgctgt ccggcggcac cgcgcagctg
     1621 ttgcgccatc ccgatcagca cgcgcgcttg gtcaacgacc tggcactgct tcccaacgcg
     1681 atcgaagaga tgctgcgctg gaccgcgccc gtcaagaaca tggctcggac gctgaccgcc
     1741 gacaccgagt tccgcggcac cgagctgaag cagggcgaga agatgatcct gctgttcgag
     1801 tcggcgaact tcgacgagaa agtgttcggc gacccggaga acttccgcat cgaccgcaat
     1861 ccgaacaatc acttggcgtt cggcttcggc acccatttct gcctcggcaa ccagcttgcc
     1921 cggctcgagc tgtcgttgat gcagacacgg ttactgcaac ggttgcccga catgcgcctg
     1981 gtgtccgagg atgcgctgcc tttgcggccg gccaacttcg tcagcggcct ggaacggatg
     2041 ccggttcggt tcaccccgac caaaccgctc accggctgac cctccaccgc gagcgtgcgt
     2101 gtctgtacgc cgacacgtcg acgaacacgt gcattccgcg cacgttcacg cgcgactaag
     2161 aaaacgtcag cgactgttga cgcttgtcgt ggttgccgtt gatcagggac gaaatcggca
     2221 ccgcgccgaa aagcgggcgc tcgcaggctt tggcgtggac actgttgatg tgggtactga
     2281 ccgcgtccac tacgcgcgaa acggcgatgt tcggttggcg taccgggtgt ttggcgacgc
     2341 ggacatcacg ctcgtctggg tgcccggctg ggtcagtaac gtcgatctct acgacgaccc
     2401 gacgacgcca ttcgcccccg tggtcgcgca actcgctcgc aagacccggc tggtcgtctg
     2461 ggacaagcgc ggtacaggac tctctgaccc ggtgacgcgc attccgccgc tcgacgaacg
     2521 catggacgac ctgcacgccg tgctcgatgc ggtggacgct gaccgccctg cgctgctcgg
     2581 tgtatccgag ggcggcccga tgagcgtcct gttcgcggcg acgtacccgg aacgcgttcg
     2641 ttcgatggtg ctctacggca ccgcggcgcg gtttgtgcgc gagccgcccg attttccctg
     2701 gggcgtcccg ccggaccgga tcgaagcagg cgtggccagc atcgacgaac actggggcga
     2761 gggttacctc gccgagcgaa ttctcgggga cgccgtcgaa gtccccgggg tacgggagat
     2821 gcttggcaga tacgagcgcg caagcgcgag tccgaccatc gcgggaatgc tgtggcggtc
     2881 gttagcggaa atcgacattc gcgagatcct caagaccgtt cgaacgccca cgctggtgct
     2941 gtcccgccct ggcgatcaga tggttccaat cgagtcgtcg caggcgttgg ctgcaggcat
     3001 ccctggtgcg gtgttccggg agttgcctcc cggcgcgcat gctgccttag atatcgatga
     3061 ctcactggcg gccgagactc ttgcgtttgt ctgcggcgaa accgagactg cggtgaacga
     3121 aagagtgctc acgactgtgt tgtttacgga catcgtgggt agtaccgagc aagttgtggc
     3181 tgcgggagat gcgcactggc gccatcgact cgacacccat gacgagctcg tggacagatt
     3241 gctgtcaaag tacggtggca agcgcgcgaa gcataccggc gatggaatct tcgcgctctt
     3301 cgacggtccc accaaggcgg ctcggtgcgc ccttgacttg gtgccagcgc tcgccacccg
     3361 tggtattcgg atcagggcgg gcgtgcacat cggggaatgc gagcggcgtg gggaggagtg
     3421 gagtggactg accgttcaca ccggcgctcg tatcggtgcg atggctggtc cgggcgaggt
     3481 gctgacgagt cgtaccgtgc gggatctttc cgcggggtcc ggcttgcgtt ttcagagcct
     3541 tggcggccac cgcctcaaag gcatcccgga agcgacggag gtgttccgag tgactgctcc
     3601 ggcgggcgcc tcgttctaat cgcaaccgcc gcactcaccc agcgcgagcg tgcgccgcgt
     3661 gccgacaacc acggcgtgtc gccgtacaga cacgcacgct cgcgcagaaa agctagcggt
     3721 tcttgaactc gggcttgcgc ttctcggcga acgccctcgg gccttccttc gcgtcgtcgg
     3781 acaggaacac ctggatccca agctgcgtgt cgatcttgaa cgcctcgttc tccggcatgc
     3841 cctcggtctc tcggatcgac ttcagaatcg cctgcacagc cagcgggccg ttgttgttga
     3901 ccgtttcggc gatctccagc gccttcgtca acgcctgacc gtcaggcacg acgtaaccga
     3961 tcagcccgat ctccttggcc tcggccgcgc tcaaatgccg cccggtcagc aacatgtcag
     4021 cggcgagcgt gtacgggatc tggcgcggca gccgcactgc cgagccgccc atcggataca
     4081 gactccactt cgcctcggaa acaccgaatt tcgcgctctc tccggccacc cgaatgtcgg
     4141 tgccctccag gatttccgtg ccgccggcga tcgccgggcc ttcgacggcc gcaatcaacg
     4201 gcttggtcag ccgccggccc ttcagcagag cgtcgatgcg cgacgggtcg tagctgccgt
     4261 ccttgaacga gtcacccggc gggcgggcag tcgccgcctt gaggtccatg cccgcgcaga
     4321 agtagccgcc ggcaccggtg aggatgcatg accggatgtc gggatcggag tcgacgcggt
     4381 cccaggcctg gaccattatc gagagcatct cggtagacag cgcgttgcgc gcctcgggcc
     4441 ggttcagtgt caagatcagg gtgtgtccgc gctgttcaat gagggcgtcg gcgcctttgt
     4501 cgaactcgct cacgggttcc caccttccta cagacttgtc aggaaatgta acacgttcta
     4561 atttggggtc cgtggccctg aatatcgcag acctcgccga gcacgccatc gacgccgtac
     4621 ctgaccgcgc cgccctgatc tgcggtgacg aacagctgac ctacgccgag ctggaggaga
     4681 aggccaaccg tctcgcgcac tacctgatcg accagggcgt caagaaagac gacaaggtcg
     4741 gactgtactg ccgcaaccgc atcgagatcg tgattgcgat gctcgggatc gtgaaggccg
     4801 gcgccatcct ggtgaacgtc aacttccgct acgtcgaggg cgaactgcgc tacctgttcg
     4861 acaactccga catggtcgcg ctggtgcacg agcgccgcta cgcggaccgc gtcgccaacg
     4921 tgctgccgga cacgccgaac gtcaagaccg tcttggtcgt cgaggacggc accgacctcg
     4981 actaccagcg ctacggcggc gtcgagttct acgaggcgct cgcgcagggc tcacccgaaa
     5041 ggaacttcgg cgagcgcagc cgcgacgaca tctacctgct ctacaccggc ggcaccaccg
     5101 gcttccccaa gggtgtgatg tggcgccacg aggacatcta ccgggtgctg ctgggcggaa
     5161 ccgacttcgc cacaggggaa tacgtcaagg acgagtacga cctctccaag caggccgcag
     5221 ccggaccgcc gatgatccgc ttcccgatcc cgccgatgat ccacggcgcg acgcaatcgg
     5281 ccacctggat ggcactgttc tccggcggga ccgttgttct cgcaccggaa ttcgacgccg
     5341 acgaggtctg gcgcacaatc aacgagcaca aggtgaacct gctgttcttc accggtgacg
     5401 cgatggcacg gccactgctc gatgcgctga gcaaagacaa cgattacgac ctgtcgtcac
     5461 tgttcctgct cgccagcacc gcggcgctgt tctcgccgag catcaaggag aagctgctcg
     5521 aactgctgcc taacagggtg atcaccgact ccatcggctc gtccgaaacc ggtttcggcg
     5581 gaacgagtct cgtcgccaaa ggtgagacga acaccggcgg accccgcgtc accatcgaca
     5641 agtggaccgt ggtgctcgac gaggacggca acgaggtcaa gccgggctct ggcgtccgcg
     5701 gcatcatcgc caaacggggc catgttcccg tcggttacta caaggacccc gaaaagtccg
     5761 ccgccacctt caaagtcatc aacggtgtcc gctacgcgat tcccggcgac tacgcgcagg
     5821 tggaagccga cggcacggtg acgatgctgg gacgcgggtc ggtgtcgatc aacagcggcg
     5881 gcgagaagat ctaccccgaa gaggtcgagg ccgcgctcaa ggggcacccc gatgtgttcg
     5941 acgcgctggt ggtcgggatc cccgatgagc ggtacgggca gtgcgtcgcg gccgtcgtgc
     6001 aaccccgcaa aggtacccgg ccgtcattga gcgatctcga cgcgttcgtg cgctccgaga
     6061 tcgcgggata caaagtgccg cgggcacttt ggtacaccga cgaggtgaag cggtcgccag
     6121 ccggcaagcc cgactaccgg tgggccaagg agcagaccga gggacggccc gccgacgagg
     6181 tcagcaccaa acacgccgga gcgaaaacct gatgcgcacc gagctgtgcg accgcttcgg
     6241 catcgactac ccgattttcg tcttcacccc gtcggagaag gtggccgctg cggtgagcaa
     6301 ggccggcgga ttgggcgtgc tgggctgcgt gcggttcaac gaggccgacg atctcgaaga
     6361 cgtcctgcat tggataccgc cgtcgacatc gacaagctta ttccgcagca acatcgggag
     6421 ttcgtcgaca aaacccttgc cgaccttggg gttccaccgc tgccggaaga tgagaatcga
     6481 aacgaaggtg ttctgggctg gcttcattcg gtcgcccggt cagatgtcga ggtggcgctg
     6541 aagcacccga tcaaactgat cgcgaatgcg ctcggctcac cgccgaagga cgtcatcgac
     6601 caggcacacg aggccggcgt cccggtcgcg gcgctggccg gcacagccgc gcatgcgcaa
     6661 cggcatgtcg acaacggcgt ggacatcgtc gtcgcgcagg gacatggggc cggtggccac
     6721 accggcgaaa tcgcgtcgat ggttgcgcga cgagtccggc gtgtcaacga tccagcgggc
     6781 gctgctgagc gcgacgtccg gatcgttcga ctcgttcatc cgttgatgcg cctcggaaac
     6841 caggatgttc tgcagcggca tcggcagggg ttcgggcgca tccggtggcg ggtatccgct
     6901 tttcttatga aagtgcgcta gctgccggcg atttcgtcga ggcgacgggt tggcggcctc
     6961 gaagccgctg accagctcgc cagtgacacc gtgcggcgtc gcatctacac cggcaagccg
     7021 gcgcgactgc tgaagagccg ctggaccgag gcgtgggacg caaccgaggc gcgggcacgt
     7081 gtccgttgga aggataaccg cggccctcgg cgtgaccgtc ccccgatcgg gggacaacga
     7141 attccccggt gcggggtccg gtcagccgac agacagcgcg gctctgcggc ggcacagtag
     7201 gtacatcgcc ggaacaaagc agcggacagg ccgcaatatt aacgcagaca ggagaactca
     7261 aaatgaccgc taaaaccacc acgtggatcc gccgagttgc cgccggcgcg gtgctggtcg
     7321 cggctcccgc gttcatcgcg ctggacactg ccgccgccag caacgccgag accagctcga
     7381 cgtccccgtc ctacgactat tcgccgacac ccagtcagac gacccagtac ccgtacgcgc
     7441 cgtggtacca gtcgttccat caacggcacg ccgcggaggt gcagtcctgg taccggtgat
     7501 cagttggccc caccccgccg catcagcgag gtatcgggaa caccggatcg tcgcactcga
     7561 tgccctgcgc ggacagttga cgcttgagca ccttgaacgt ctgcgtgcgc ggcaactccg
     7621 cgctgatccg cacgaacgac ggccactgct tcggcccgag atcgggctgc tcaaccaaga
     7681 acttgcggaa cgcgtcgggg tcgaacgtca cgccttccgt gagcaccaaa gccgccatca
     7741 cctgatcgcc cacatcgggc gccggcacgg gatacacggc tacctccacc acgtcggcgt
     7801 tgcgcagcag cacgcgttct atcggtgcgg cgccgaggtt ttcgccgtcc acccgcatcc
     7861 agtcgccgag ccggcccgcg aaatacacgt accccgccac gtcgcggcag gccaggtcac
     7921 cgctgtggta gacaccgccg gccatgcgct cggcgtcggc gtccgggtcg ttgtagtacc
     7981 cctcgaacct gccagccccg ccgatgttca ccagttcgcc ggtcaccccc ggtggacagg
     8041 gctcgccggt gtcgacgtcg acgatctcga cgccgtcagg caacggaccc aacgagcccg
     8101 gcggggtgtc gggggtgcgg gcgatggcca ctccgccctc ggtggacccg aagccgtcga
     8161 ctaccaccgt gccgaaccgg cgggcgaacc gctcgatgtc gccgggagcg ccttcgttgc
     8221 cgtacaccgc cttcagcggg ttgtccgcat cgtcgggacg ctccggcgtg gcaagcacgt
     8281 acgacaacgg cgtgccgacg tagttcgcgt acgtcgcgcc gtagcggcgg acgtccggaa
     8341 tgaactgcga agccgagaac ttgcgccgca gaaccatcga acctcgacac gccaacgcca
     8401 ccgaccaccc gaccagcacc gcgttcgaat ggaacagcgg catcgagacg tagctgatgt
     8461 cgctcgggcc gagggagaac cgttccgtca tcgtcacgcc ggcgatcgcg accttgccgt
     8521 ggctgcagat caccgccttc gggtcaccgc tcgttcccga cgtgtagatc agcatgaacg
     8581 ggtccgtcgg cgcagcggac cgcgcgacca ccggcgcatc ctggtaggcc tgcagttcag
     8641 cggcccaatc cgcggaatcg acgtcgatgt agtcggtgtc gctcaaccct gcggccaatg
     8701 ccgaatccgc cagcaccact tggcagtccg catgccgcac gtcacggtcc agcgcggcgc
     8761 cgcggcgaat cgggttcagg cccaccggaa caattccgga cagcgccgcc gcgatcagca
     8821 gcgaggagaa gaacggcgtg ttctgcagca gcaccccgac atgcggcggc cgggccgggt
     8881 cgaggcgctt gcgtaacgcc gcggcgactg ccgcgccccg cgcaatgtgg ttgcgccagc
     8941 tgacgaacga gtcctgattt tctcctcttc gcgcaagcgg ctcatcggcg ttgtctcctc
     9001 ttcgcgcaag cggctcatcg gcgaagtaca cgccgcggtc gtcgacgtcg acgagcggtg
     9061 tgatcagcga cgtgaccgtg ggaaggtcgc tgcctgatgc cgggttcaag ccggtgtgtc
     9121 agctagttcc cggccgatgc gcagcagctg cccggtggcc ccgcccaccg cgaactcagt
     9181 ctgcttggcg gcgaggaaat accggtgcac cgggtggtcg aggtcgatac ccacgccgcc
     9241 gtgcacgtga accgtggtgt gcgcgacccg gtgcccggcg tcggcagccc agaacgccgc
     9301 ggtggccacc tccaggtcgg ccggaagatc ctcggacaac cgccacgccg cttgggtcgt
     9361 ggtcaaccgc aagcccttca cgtcgatgta gccgtcggcc aggcgctggg ccactgcctg
     9421 gaaactgccg ataggtctgt cgaactgttc tcgctcacgg gcgtattcgg ccgtcagctc
     9481 caacgcccgc tccaacacac cgagttgaaa tgctgtgcgg cctagcgtgg catgcgtaac
     9541 aagccacccg acaacctcct cgccaccgac aacccgctcg gaatccactt cgacgccttg
     9601 cagttccagg tgcccgacgc tgccgtgtcc ggtggtttgc agcgacgtta ccgacacccc
     9661 agcatcggcc gcagcgacga ggaaaacctt gatgcccgaa tcggtttcgg acggcaccag
     9721 gaacgcatcg gcgacgggtc cgtaggcaac ctgagtgcgg gtgccggtca accgataccc
     9781 gctaccgctc gccgtcgcct gcaccggtcc ttcacccatt tcgccgtcga gcgcgaccgc
     9841 gaggatcttc tcgccattga ccgccgggga agcccactct tgttgcagcg ccgccgaacc
     9901 gaacttggcc agtgcgcccg cggcgagcac cgccgagtcg aagtagggca ccgcggccat
     9961 ctgccggccc agcgctacca gcacggcaac ctgctccagc acaccaaaac cgccgccgcc
    10021 caacgactcc ggtgccgcgg cgctcagcac gtccgaatcg atcagggtgc gccacaggtc
    10081 acggtcgaag cgttcgtcga gcccgtccag ctcccgctgg tgttccggcg tgcaaaccga
    10141 ctcggtgatc gtgcgcacca ggccaccgag atccgtcgca gcttcggtcg tcttgaaatc
    10201 cattgtggtt ccttaccggt tcactcgggg caggcccagc gcaaccatgc cgatgatgtc
    10261 gcgctgaatt tcgttggtgc cgccgccgaa cgtcaggatc agacacgcgc gatgcatccg
    10321 ctcgacccgg ccgcgcagca gcgcacccgg cgagttcggt cgcagcgtgg ccgcgctccc
    10381 cagcacctcc atcagcaacc ggtaggcctc ggtggccagc tcggtgccga acaccttggc
    10441 cgctgaggcg tcggccggcg acggggacgc gttgtccgat gacgccagct cccagttgat
    10501 cagtttaagc acttcggctt tggcgtgtac acgcgccagg ttcagctgca cccattccga
    10561 gtcgatcaac cgggacccgt gaatgtcctt ggtgttctgc gcccattcac gaaccgcgtt
    10621 gagcgccaag aagatcggct gggccgacac cagcgcgacg cgttcgtggt tgagctggtt
    10681 ggtgacgagc ttccagccgc cgttctcctc gccgacgagg ttggtcaccg gcacgcgcac
    10741 gtcttggtaa taggtggcgc tggtgtccgg gcccgccatc gtgtgcaccg gggtccacga
    10801 gaagccgggc gcggtggtcg gcacgatcag catcgagatg ccgcggtgtt tcttcgcctc
    10861 ggggttggtc cgcactgcca gccacacgta atcggcgtac gggatcaggc tggtccacat
    10921 cttctgcccg ttgatcacat actcatcgcc gtcgcggacc gcggtggtgc gcagggcggc
    10981 caggtcggtg ccggcgcccg gttcggagta gccgatcgag aaatgcagtt caccggacgc
    11041 gatcttgggc aggtagaact tcttctgctc gtcggtgccg aacgccatga tcgtcggcgc
    11101 cacactgttg atggtcaaga acggcaccgg cgctccggcg atggccgcct cgtcggtgaa
    11161 gatcagctga tccatcgcgg atcgcgcctg gccgccgtac tcggtgggcc agcccagtgc
    11221 gagccacccg tccttgccca tctgggcgac ggtgtcccgg tacgcattgc cggtgccgta
    11281 ctcaccttcg accgagctca gggcctcgac ccgctcgggc gtcatcaact tgccgaagta
    11341 ggaccgcaac tcgcggcgca actcctcttg ttcggggccg tagccgatcc gcatcgcctc
    11401 gtcctttggt ttgaatggaa cccctggcat acccatctgc aatcccggtt gtaacacgtt
    11461 ctagtcttgt ggtccagcga cggtttccgc cagcgggccg ccgacctggt cgtattgtgg
    11521 gtgcggcgtc aagaccgggc cgtccgggtg agtacgagga ggttgtcgtg cgagttgaag
    11581 tggaccgcga tcgatgtgaa ggtaacgccg tgtgcgtggg aatcgcgccg gacctgttcg
    11641 atctcgacga cgaggactat gcggtggtga agaaggatcc ggtgccggcc gacgaggagg
    11701 acctcgccga gcaggcgatc gccgaatgcc cccgggctgc attgcttcga aaggactaga
    11761 ggtattcata agttgagcgg atccgagggg tacgaggctg gccgtcaggc tgacgaactg
    11821 accgatttgt cggggcgcgt cgcggtggtc accggcgccg ccgccgggct gggccgagcg
    11881 gaggcgatcg gcctggcggc tgtcggcgcc accgtggtcg tcaacgacct cgacgcggca
    11941 ctaaacgatt ccgacgtctt cgacgagatc aaggccgccg gcagcaaagc cgttgccgtg
    12001 gcgggcgacg tcggcgaacg gtcaaccgcc gacgagatgg tggccgcggc cgagcgcgaa
    12061 ggcggcctcg acatcgtcgt caacaatgcc ggtatcaccc gcgaccggat gctgttcaac
    12121 atgtccgacg aggactggga cctcgtcatc aaggttcatc tgcgcgggca tttcctcctg
    12181 acccgcaacg ccgccgccta ctggcgctcg cgtgccaagg agtcaggcgg ccagacctac
    12241 ggccggctca tcaacacgtc atcggaggcc gggctttcgg ggccggtagg tcaggccaac
    12301 tacggtgcag caaaggccgg gatcacggcc ttgacgctgt ccgcggccag ggcgctgggc
    12361 cgctacggcg tatgcgcgaa cgcgatctgc ccgcgggctc ggacggcgat gaccgcggac
    12421 gtgttcggcg aggcgccaga cgacgccgat atcgatccgc tctcgccgga ccatgtggtg
    12481 acgttggtgc gctttctcgc gtcaccggcc gcggaggctg tcaacgggca ggtgttcatc
    12541 gtctacggtc cgtcggtgac gcttgtcgcc gcgcccaccg cagtgcaccg gttcaccgcg
    12601 gaagggccgg cgtgggcgcc gcaagaactt agctcagcaa tgcaaaaatt ctttgctgac
    12661 cgtgacccgg acatgacatt cgcggcgacc caattgatgg agcggtagcg gcgacgtccg
    12721 tgtcaaacgc ggggtctgaa acgagtagaa cacgttacag accccgcgcc cgagaactcg
    12781 ctctgaaatg gacaaacgtg caagttatga cacttttagt gatctttgac accgcgaacg
    12841 tgttctagtt agtatgatcc ggctcacaca gcgtcacgtc ctgttgaggg gtgggaaggg
    12901 cgccggaccg gccagtttcc tggcttcgcc cggaatctgt caccgcatcc ctgtgcccgg
    12961 gaaggggggc cgagtgaagg atcagctcgc ggttcccgct cgggccgtgg gcggcttctt
    13021 cgaaatggct ggggacacgt tccgcgcgat tttccgccga ccgttccaat tcggtgagtt
    13081 cctcgagcag acatggatga tcgcccgcgt ctcgctggtg ccgacccttc tggtgtccat
    13141 cccgttcacc gtgctggtcg cgttcacgct caacatcctg ctccgcgaga tcggtgccgc
    13201 cgacctgtca ggagcgggca ccgcgttcgg caccatcacc caactcggtc cggtggtgac
    13261 ggtgctggtg gtcgccggcg ccggcgccac ggcaatctgc gccgacctcg gcgcccgcac
    13321 catccgcgag gagatcgacg cgatgcgggt gctcggcatc gatccgatcc accgtctggt
    13381 ggttccgcgc gtgctcgcgt cgacgctcgt ggcactgctc ctgaacggat tggtctgcgc
    13441 gatcgggctg tccggcggct atgtgttctc ggtgctgctg cagggcgtca acccgggcgc
    13501 gttcatcaac gggctgaccg tgctgaccgg tctcggcgaa ttgatgctcg ccgaggtgaa
    13561 ggcgctgctg ttcggtgtct gcgcgggtct ggtgggctgc taccgcggcc tcaccgtcaa
    13621 gggcggaccc aagggcgtgg gcaacgcggt caacgagacc gtcgtctacg cgttcatctg
    13681 cttgttcgtg atcaacgtcc tgatgacggc ggtcggcgtg cgagtgctgg cgcgatgagc
    13741 tatgacgtaa cccttcgcct gcggcgcctc ttctctgggg tgccgaaagt ggtcgacagc
    13801 ttcggcgagc aggcgctgtt ctacgccgag tcgatccggt ggataccgaa cgccgtcacc
    13861 cactaccgca aggaaacggt gcggctcatc gccgagatga cattgggcac cggcgctctg
    13921 ctggtcatcg gtggcaccgt cggcgtggcg gcattcctga cgctggcctc cggcggggta
    13981 atcgccgtgc agggttattc gtctctcggc aacatcggca tcgaggcgct tacgggcttc
    14041 ttgtcggcct tcctcaatgt ccgcatcgtc gcaccggtca tcgccggcat cgcgctggcg
    14101 gcgacaatcg gcgccggcac caccgcgcag ctgggcgccc agcgggtggc ggaagagatc
    14161 gacgctttcg aggcgatggc catccactcc gtttcctatc tggtgtccac caggatcgtc
    14221 gccggcttga tcgcgatcgt tccgctgtac tcgctgtcgg tacttgcggc gttcttcgcg
    14281 gcgcgcttca ccactgtcta catcaacggc cagtccggcg gcctgtacga ccactacttc
    14341 aatacgttcc ttgtgccgac cgacctgctg tggtcattcc tgcaggccat cgcgatgtcg
    14401 atcgcggtga tgctggtgca cacctactac ggttacaacg cgtccggcgg gcccgtcggc
    14461 gtcggtatcg cggtgggaca ggcggtacgg acgtcactca tcgtggttgt tgtgattact
    14521 ttgttcatct ccctggccgt ctacggcgcc tccggtaact tcaacctttc cggatagagg
    14581 gggagcatgg cgaattccgg gtcaaagacc cgccacggcc ggatcgcagc cgcggtgctc
    14641 gcgactttga tcctggcctt cgcagtgctg acctacctct cttatacggc ggccttcacg
    14701 tccaccgact ccgtgaccgt gacctcgccg cgcgccggcc tggtgatgga gaaggacgcg
    14761 aaggtcaaat accgcggcat ccagatcggc aaggtcaagg acatcgcata ttcgggcgac
    14821 caagcaaagt tgacgctcgc gatcgacagc aaccagatgc gctacatcga ttccaacgcg
    14881 gaagtgcgga tcgccagcaa cacgatcttc ggcgccaagt cggtggaatt cgttccgccg
    14941 accaagccgt cgggaacgtc gctgcgccca gacgcgcggg tgcaggtctc ggcggtgcaa
    15001 ctcgaggtca acacgttgtt ccagacgttg gaagacctgc tgcacaagat caacccgatc
    15061 gacctgaacg cgacgctgtc ggcgatcgcc gaaggactac gcgggcacgg cgacgacttc
    15121 ggtgcctcgc tcgccgggct gaatgaattg ctgccgaaga tcaacccgaa gatgccgacg
    15181 ctgcagtccg acttccagca ggccgccgcg gtcggcaaca tctacggcga cgccgcgccg
    15241 gatctggtca ccgttctgga caacgtcccg acgatcagcg acaccctcgt ggatgagcag
    15301 gaaaatctca acgccacgct gctcgcggca accggcttgg cggacaacgg aactgcaacg
    15361 ctggagcccg ctcaagagga cctgatcgcc gcccttcagc ggttccgcgc accgttgaag
    15421 gtgttgtcgg actactcacc ggagttcggc tgcatcttca caggcgtcaa gaaggccctc
    15481 gaccagttcg cccccgtcat cggcggcacc aggcccggcc tgttcacgtc gtcgaacttc
    15541 gttttgggcg ttccgggcta cacctacccg gagagcctgc cgatcgtgaa cgccactggc
    15601 ggtcccaact gccgcggtct tcccaacatc cccaccaagc aattcggcgg atcgtggttc
    15661 cactcgccgt tcttggtcac cgataacgcg tacatcccgt accagccgct caccgaggtg
    15721 caggtcgatg ccccgtcgac cctgcagttc ttgttcaacg gcgcgttcgc cgaaagggat
    15781 gattggtgat gagcgctcgc gcgaagaaca gagggcactg atgagcgctt gcgcgaagaa
    15841 gagagaatcc tgatgccggg gcaccgcgga gtcctcatca aggtcagcat cttcacggtc
    15901 gtgatgctgc tggtcgccgc gggcttggtg gtgatcttcg gacagttccg cttttcgtct
    15961 accaacacct accacgcgat cttcgagaat gcctcgcggc tcaagggagg gaacgacgtt
    16021 cggatcgccg ggatcccggt gggtagcgtc aaggacatcc agctgaagcc cgacaacaag
    16081 gtggacgtca cgttctcggt cgacaagcgc taccagatgt acacgtcgac caaggcggtc
    16141 atccgctacc agaacctggt cggcgaccgc tatctggaga tcacgtcggg gccgggtgag
    16201 cttcgaaaag tgccgccggg cggcacgatt cagcacaccg agcccgcgct tgacctcgac
    16261 gccctgctgg gcggattgcg tccggtgctg aagggcctga gcggcgacaa ggtcaacgag
    16321 atcagcagcg cgatcatcga actgctgcaa ggccagggcg gtgcgctgtc ggatctgctg
    16381 tcgagcaccg gctcgttcag tcggacactg gcggatcggt atcaggtcat ctccgacgtg
    16441 atccacaacc tgaatacggt gctcaccaca gtcgacgaga agagcgccca attcaacgag
    16501 agcatcgatc agctccagca attggtgacc ggtttggcgc aagggcgaga tccgatcgcc
    16561 ggtgcgctgc agccgctcgc gtctgcggcg tcggatctga cagacgtgct gcaaagctcg
    16621 cggcgcccca tccaaggcgt catcgagaat ctccgtccct tggcaaccga aatggacaac
    16681 cgcaggggcg agatcaacgc ggtcgtcgag ccgctcgccg agaactactt gcggctcaac
    16741 gcgcttggcg cctacggcgc gtacttcaac atcttctact gctcggtgcg catcaagatc
    16801 aacggcccgt ccgtcggccc gcccaccggc acgatgtcgg acagcgacat cctgattccg
    16861 cagggcggac cgcctgaccc gtcgaagggc aggtgctccg agaatggctg agggccgcaa
    16921 tccgcttcga accggcgtca tcggcctggt gttggtggtc ggcgtggtgc tggtgtcgtt
    16981 cggctacaac aagctgccgt tctggccgca gggaaagaca tacgaggcgt acttcaccga
    17041 cgccgggggc atctcgccgg gtaacgaggt caacgtgtcg ggactgaagg tgggccaggt
    17101 gagcagcgtc gcgctggccg gcgacgtcgc caaggttacg ttcaccgtcg accgcaagat
    17161 caaggtgggc gaccagtcgc tggtgtcgat caagacggac acgatcctcg gagagaagtc
    17221 gctgtcggtc acgccgggtg ggcgggggga ctcgacgacg atcccgctga accgcacgac
    17281 ggcgccctat acgttgaaca acgctctgca agacctcggg cagaccgcca gcgatctgga
    17341 caaggacaag ttcacacaag ccctgggcgt gctgacggat tcgctgcacg aggcgacacc
    17401 ccagctgcgc caggcgctcg acggcgtcgc ggcgctgtca cgcagcatca atgccaacga
    17461 cgaggcactg gcgcagctgc tcgagcgggc caggtcggtc accaaggtgc tggccgaccg
    17521 agccggtcag gtgaaccggc tcatcgtcga cggcaatcag ctgttcgccg ccctcgacga
    17581 acgccgcgct gcgatcagca cgctgatctc cagcatcgag ccggtctccc aacagattga
    17641 gggcttcgtc gcagacaacc gcagggagtt cggcccggcg ctgcggaaac tcaacgcggt
    17701 actcgacaac ttgctccaac gccgcgaaca catcagcgaa gcgcttcggc gcctgccgcc
    17761 gtatgccacc gcgctgggtg aggtggttgc gtcggggcct gggttccaca tcaacctgta
    17821 cggtctgccg cccggcccgc ttggagaggt gctgctggac ttgtacttcc agcccggaag
    17881 gctgcctgcc agcctggcgg actacttgcg cggcttcatc tccgagcgct tgatcatcag
    17941 gccgaagtca ccatgaacgc gcagccggca tctcccggaa tgaggtggtt gcgctacggc
    18001 gccatcaccg ccctggtggc ggcgtttatc gccggcatct acgtgatctg gccgggccgt
    18061 gccggtcaca aggtgatcgg gtacttcacg tcagcggtcg ggttgtaccc cggcgacgac
    18121 gtccgcatcg tcggcgttcc ggtcggcacc atcaaatccg ttgagccaca agcgcaggcg
    18181 gtcaagatca cgatgtcggt caaagacggg gtcaagatcc cggccggcgc gcaggcggtc
    18241 atcatggcgc cgaacatcgt gtcggcgcgc ttcgtccaac tcacccccgc ctacaccagc
    18301 ggtcccgtga tggcggacgg cgccaccatc ggcctggacc gcacggcggt gccggtggaa
    18361 tgggacgaag tcaagtcgga gctgacaaag ctcagtgcat cgcttgggcc acaagccgga
    18421 tcgatgcaag gaccgctggg cgatttcgtg aaccaggccg ccgacacgtt cgacggcaac
    18481 ggcgattcct tccgccaagc gctgcgggag ctgtcccaga cgacggggcg gctgggtgat
    18541 tcccgcaccg atctgttcgg caccatcaag aacctgcaaa tcttgatcaa
//
LOCUS       DASWBF010000378         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_162745,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000378 DASWBF010000000
VERSION     DASWBF010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162745"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(993..1364)
                     /locus_tag="VF874_14090"
     CDS             complement(993..1364)
                     /locus_tag="VF874_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177151.1"
                     /translation="MALIRRLAMRRQVRRTSEQALAATPLFSTPHRPPDTPSALPATI
                     PPPPPRVEPAQLSEPSPPRSAPAATNAAPVANDKEDHVDAPAPTDPLASDPSGSTAQN
                     WALPEWRLTRRARARAPRRQL"
     gene            complement(1374..2060)
                     /locus_tag="VF874_14095"
     CDS             complement(1374..2060)
                     /locus_tag="VF874_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177152.1"
                     /translation="MSNANKVIRPARDPRWSRKRLIGMLLDCYGPTARGGVDVAAVAD
                     YAAVSPSTVRRWISRGHPHGSSRPLAIPKRRIVQLQRGPAEVERRNEQQYQHALNALA
                     SIDDERSILPAWREQGWLDQHTVAIIEIHRKPWLQVAVTNANPRAMAELLRRGALVDS
                     ITVPTRFHAQVLAHAVMTRQEAWRVHPTPQRLAAGRTQVWMADAPPVDLSALADDLRS
                     GSATTDNLPR"
     gene            complement(2057..2776)
                     /locus_tag="VF874_14100"
     CDS             complement(2057..2776)
                     /locus_tag="VF874_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177153.1"
                     /translation="MARSTRRRTSIGADDLAGYGSVANGTVDVDRAARGLGASKTEVR
                     QAIRQAEAAQSNTFVRRISGRREADSAEGRSVRGMLQAVFGRGPRGGAVNANAAAREL
                     GVSPGTVRRWAAGTQKPSTGRLAAIRAAARRATSTKRGRRAATSDFRSSAQGSQALRA
                     GNKIWVSGEQGVGGYEQGYARDRRVAADISPEEIEALLRAYEEGGDRGLRDWLKEFFD
                     DKYVAGWDFVTIDDFGIGTPE"
     gene            complement(2776..3177)
                     /locus_tag="VF874_14105"
                     /pseudo
     CDS             complement(2776..3177)
                     /locus_tag="VF874_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211253.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          453 a         1052 c         1095 g          577 t
ORIGIN      
        1 aaaagtacat cgcgtaatga gctgggataa tccaacgatc ccaacactgt tcactcatac
       61 tgacccagag gtgggatcgt tcggcttctg tgctagcccg tactcacgca ccagcgaccg
      121 gttggtctcg cgcagaacct ccacatcgtc ctccagcgac atgatcgtcc tgtccttctc
      181 aagtagttca acttcgagtt gagcgagtcg cgtccgcagc tccagaacca ctgggtgctg
      241 atcgatcacc gatgggtcga ctgtcgctcc ctgtgcgccg aggcgccgtt ccagggggca
      301 ctgtcaaact tgagcagtcc gagggcgatt ttcgccggtt cgcctccgcg attcaccagg
      361 tcgcgtgtga cgtatgaggc ttgtgcggtt agcagaacat ccacacgacc tcaagttgac
      421 agtgccccca ataccgagtg gctcaagcac ggcgcagtcg aactcgacga acacggtttc
      481 attcgcaccg gcataccggc tgccgatcgc gcatcacctg ccgttcccct ggccaccaac
      541 cagccgggca tcttcgctgt cggcgacgtg cgcgccgact cgatcaagcg agtcgctccc
      601 gccgtcggtg aaggctccgt cgtggttgtc atcaattcac gatgaactca ccgtcgccga
      661 atgaccttgc aggcggccca tgggcagccg ggccgcagac cgcttgctcg ccgagtcggc
      721 catggcgact gccgcgttgg ggcgtgctcg cacagatctg cgttaacgct cccgcgccgg
      781 gcgcgggcag tggtgggccg ccgccgtcgc ccagccggct ccgacggttt ggggttggcg
      841 cctgcgcctg tgacgtggag tcgcctctcg acctccccgt cgaggatttg aaccggttcg
      901 tgagactgag acaaatcggg ttgaaatcgc attgagactg ccgaataagc cacaggtcaa
      961 cggcgcccgg cgatgtcacc gagtgtgaga acctacagtt gacgccgagg tgcgcgtgcg
     1021 cgtgctcgtc gagtgagccg ccattccggc aacgcccaat tctgcgcggt cgaacccgac
     1081 gggtcagacg ccagcggatc ggtcggagcc ggcgcgtcga cgtgatcctc tttgtcgttg
     1141 gcgacgggtg ctgcattggt cgccgcaggc gctgatcgcg gcggcgaggg ttccgacagc
     1201 tgtgccggtt cgacgcgcgg tggtggcggt gggatcgtcg ccggtaaggc cgacggggtg
     1261 tcgggagggc ggtgcggggt cgagaacagt ggtgtggccg ccaacgcctg ctcggaggtg
     1321 cgccgaacct ggcgcctcat tgccaggcgc ctgatcagtg ccaccggctg agcctaacga
     1381 ggcaggttgt cggtggttgc gcttcctgat ctgaggtcgt ccgctagcgc tgagaggtct
     1441 accggcggcg cgtcggccat ccacacctgc gtgcggccgg cagcgagccg ctgcggtgtg
     1501 gggtgaaccc tccaggcctc ttggcgtgtc atgacggcgt gcgcgaggac ctgggcatga
     1561 aaccgcgtcg gcaccgtgat gctgtcgacc aaggcgccgc ggcgaagcag ctcggccatc
     1621 gcacgcgggt tggcgttggt gacagccacc tgcagccatg gcttccggtg gatctcgatg
     1681 atggccacgg tgtgctggtc aagccacccc tgttcgcgcc aggccggcag gatcgatcgc
     1741 tcatcgtcga tcgacgcgag tgcgttgagc gcgtgttggt actgctgttc gttgcgtcgc
     1801 tcgacctcgg ccgggccgcg ctgcagttgc acgatccgac gcttcggtat cgctaggggg
     1861 cgagatgatc cgtgtgggtg cccgcgggag atccagcggc gaaccgtgga cggtgagacg
     1921 gcggcgtagt cggcgaccgc ggcaacgtcg acaccgccgc gagcggtggg gccatagcag
     1981 tccagaagca tgccgatgag cctcttgcgg ctccagcgtg gatctcgtgc cgggcgtatc
     2041 accttgttgg cgttgctcat tcgggtgtgc cgatgccgaa gtcgtcgatg gtgacgaagt
     2101 cccagccggc gacgtacttg tcgtcgaaga actccttgag ccagtcccgc aggccgcggt
     2161 caccgccctc ctcgtaggcc cgcagcagag cctcgatctc ctcggggctg atgtcggcgg
     2221 cgacgcggcg atcgcgcgcg tagccctgct cgtagccacc gacgccctgc tcaccgctga
     2281 cccagatctt gttgccggcg cgcaacgcct ggctaccctg cgcgctgctg cggaagtccg
     2341 atgtcgccgc gcgccgcccg cgtttggtgg acgtggcgcg ccgcgcggcc gcccggatcg
     2401 cggccagccg gcccgtagac ggtttctggg ttccggctgc ccagcggcgc accgtgcccg
     2461 gggaaactcc aagttcccgc gcggcggcgt tcgcgttgac cgccccgccc cgcgggccgc
     2521 gcccgaacac cgcctgcagc atgccccgca cgcttcggcc ttcggcggag tccgcctcgc
     2581 gccgcccgga gatccgtcgc acgaacgtgt tgctctgggc tgcctccgct tgccggatcg
     2641 cttgccgcac ctcggttttg gacgcaccga gaccgcgtgc cgcgcggtcg acgtccaccg
     2701 tgccgttggc gaccgacccg tagccggcga ggtcgtcggc accgatgctg gtgcgccgcc
     2761 tggtgctgcg ggccatcagc ggctcccgtc atcgtcggcg gtgtgtggca atgctgcgcg
     2821 ccattgggcg gcggggatca ggtcacgctt gccgcggtag tcgcggccgt tgagatggtc
     2881 gaggtgctcg gacagcagcg cggagccttc cggcttgtac tgtccgaagc cgcggccgaa
     2941 cgactgcggg ccgccgggcc acgctgtcac cgggtccggg tcctcggagg cgtacaggac
     3001 ggtgtcggtg gccaccgcca gcggccactg cccgtccttt cgccgatctg gtgcagccgg
     3061 tagacgatgt tggccttggc cttggccagc acgtgcagcc gccgctcggg gctgtagccg
     3121 gtcttgccct tgaggtgctc gtcggagccg atgatgccga ttccgtgggt gcggatg
//
LOCUS       DASWBF010000379         7563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_246337,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000379 DASWBF010000000
VERSION     DASWBF010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246337"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1118)
                     /locus_tag="VF874_14110"
     CDS             complement(186..1118)
                     /locus_tag="VF874_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HEX9177154.1"
                     /translation="MSEVLSEASWTQRRDSHHERVTALVGTYLDRRGHGTSHPVVDFL
                     FTYYRLRPAQLARWHPGFGVSLTGPASQAYARWSGYRRTDGAVTVDPDLLARRRPTVE
                     FVVGLLDATAGRPAHFGCFGLHEWAMVYRAAPAEVRHHAVPLRLSSAGTDAVVESMPL
                     RCTHFDAFRFFTGAARSRNGAQLTRQTQQATEQPGCLHATMDLYKWGAKLLPLLDSEI
                     VMDAFELAYRARVLDMRASPYDLSAFGYHPICIESPSGRAEYVRQQAAIAERAAAVRQ
                     ALLTKCRALLETVGQSFNTRDRSQRCTSPAGCPA"
     gene            complement(1111..2163)
                     /locus_tag="VF874_14115"
     CDS             complement(1111..2163)
                     /locus_tag="VF874_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9177155.1"
                     /translation="MADFLGVVLTVLLLLANGFFVGAEFALISARRDRLEALAEQGKR
                     SAVTVIRAGQNLSLMLAGAQLGITVCSILLGRIGEPAVAHLLEKPFDLLGVPDALLHT
                     VSFFVAITIVVVLHVLLGEMVPKNIAIAGPESAAMLLVPPYLWWVQATRPVIAFYDWC
                     AHVTLRAVRIEPKTELENTVSMVELSEMIAESRSEGLLDPEEHMRLSRALQIRNRVVS
                     DVAIPLNEVHMVEIAADSDGPTVEAIQRALGETGYSRFPVVDSTGRFVGYVHIKDVLP
                     AVDDPEAVVDRSTVRPLPDVSESMPLPDALSRMRRNNSHLALVTGSHDRVVGIVALED
                     LVEDLVGTVRDGTHRV"
     gene            complement(2163..3524)
                     /locus_tag="VF874_14120"
     CDS             complement(2163..3524)
                     /locus_tag="VF874_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9177156.1"
                     /translation="MSVTLILLALVGFVVLTLGTAVFVSAEFSLTALERSTVDANARS
                     GGRRDKMVQRAHRTLSFQLSGAQIGISITTLITGYLAEPLIARLLRPVLTAMSVPEHW
                     IHGTALALALLIATSVSMVFGELVAQYLAVARPLPTARVVAGPQVLFSLLFTPLIHLT
                     NGTANWILRRLGIEPAEELRSARSPQELGSLVRNSARRGALDPLTAALVDRSLQFGAR
                     TAEEFMTPRSKIESLEADDTVADLVAKAMQTGYSRFPVTEGDLDETIGLVHVKQVFEL
                     PHDDFARTRLADVARPVAVVPSTLDGDSLMAQIRANGLQTALVADEYGGIAGMVTVED
                     LIEEIVGDIRDEHDEPTPDVFRSGDGWRVSGLLRIDEVAAATGFRGHEGEYETIGGLV
                     MHELGHIPEVGESVELKAFEPEGTLDDPIRWRATVARMAGRRIDLLDLVKLGRRQGDS
                     GGD"
     gene            complement(3797..5233)
                     /locus_tag="VF874_14125"
     CDS             complement(3797..5233)
                     /locus_tag="VF874_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HEX9177157.1"
                     /translation="MRFVEGHPPPYDLTYTDVFIVPNRSDVTSRFDVDLSTIDGSGTT
                     IPVVVANMTAVAGRRMAETVARRGGIVVLPQDLPITAVKHTVEFVKSRDLVADTPVTL
                     EPDDSVSDACALIHKRAHGAAVVVFEGRPIGLVTDAVCAGVDRFTRVRDVLVRDFVTA
                     PAHTDPRQVFDLLEHAQIDVAVLTEPDGTLAGVLTRRGALRAGLYTPAVDDAGRLRIG
                     AAVGINGDVGARATALVEAGADLLVIDTAHGHQTRMIDAIKAVTSLGLGVPVAAGNVV
                     SAEGTRELLTAGATIVKVGVGPGAMCTTRMMTGVGRPQFSAVHECASAAAQLGGFVWA
                     DGGVRHPRDVALALAAGASNVMIGSWFAGTYESPGDLLRDRDDQPYKESYGMASKRAV
                     AARTTTDSAYERARKALFEEGISTSKMGLDPDRGGVEDLLDHITSGLRSTCTYVGAST
                     IAELHELAVVGVQSAAGFAEGHPLPTGW"
     gene            complement(5245..5475)
                     /locus_tag="VF874_14130"
     CDS             complement(5245..5475)
                     /locus_tag="VF874_14130"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9177158.1"
                     /translation="MTALAHVGLCVSDLDRSQRFYAEGLGFEVQERRSGGDDFAAVAE
                     VEPPVTLVAQFIVKDGTRLELLQRAPKPATTA"
     gene            complement(5472..6932)
                     /gene="gndA"
                     /locus_tag="VF874_14135"
     CDS             complement(5472..6932)
                     /gene="gndA"
                     /locus_tag="VF874_14135"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX9177159.1"
                     /translation="MTPPEADTTGTAQIGVTGLAVMGSNIARNFARHGYTVALHNRSI
                     AKTDALLAHYGSEGKFVRTETMAEFVAALEKPRRVLIMVKAGDPTDAVIDELAGVMDK
                     GDIIIDGGNALYTDTIRREKAMAERGLHFVGAGISGGEEGALNGPSIMPGGPAESYKS
                     LGPLLEEISAHVDGVPCCTHIGPDGSGHFVKMVHNGIEYSDMQLIGEAYQLLRDGLGL
                     SAPQIADVFAEWNKGDLDSYLIEITAEVLRQTDAKTGKPLVDVILDEAEQKGTGRWTV
                     KSALDLGVPVTGIAEAVFARALSGSVAQRTATTGLASGSLGGKPSDADEFTEDVRLAL
                     YASKIVAYAQGFNQIQAGSAEYDWGVTPGDLATIWRGGCIIRAKFLNRIKDAFDDDPD
                     LATLIAAPYFRSAVEAGIDSWRRVVATATRLGIPIPGFASALSYYDALRTERLPAALT
                     QAQRDFFGAHTYERVDAERGKKFHTLWSGDRSEVEA"
     gene            complement(6991..>7563)
                     /locus_tag="VF874_14140"
     CDS             complement(6991..>7563)
                     /locus_tag="VF874_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX9177160.1"
                     /translation="RAHHRMLVDLLGAAGTGHLRIIDVSEPLAYCLPGVRSRVVVSKG
                     ALNTLSDKEITAILRHERAHLRARHDLVLEMFTAVHAAFPRFVRSARALDAVRLLVEM
                     LADDAAVRVAGPTPLARALVACAAGPTPSGALAAGGPSTVARVRRLSGRGNSLAVATT
                     AYLAAAAILVVPTVALAVPWLTELHRLFTA"
BASE COUNT         1214 a         2621 c         2566 g         1162 t
ORIGIN      
        1 tgagtgcacc gaaaatgcct tcccgaactc tggacaagat cgacatcgac actcgtattg
       61 tcccagttca gaaggcattt tcgcggttca cacgccgatc aaatcgccgg cccatccacg
      121 gattcaggct aagtcggtgg acgtgcgcac cgatccgctt ccgaagtccg gcgcgggcaa
      181 gatcctcaag caggacatcc ggcgggagat gtgcaacgct gcgaccgatc tcgcgtgttg
      241 aagctctgac cgaccgtctc caacaacgcg cggcacttcg tcaacagcgc ctgacgcacg
      301 gccgccgccc gctctgcgat ggccgcctgc tgacgcacgt attcggctcg gcccgaaggg
      361 ctttcgatgc atatcgggtg gtagccgaaa gcgctcaggt cgtaggggct ggcccgcatg
      421 tccagcaccc gcgctctgta ggccaactcg aacgcgtcca ttacgatctc ggagtccagc
      481 agcggtagca gcttggcgcc ccacttgtag agatccatcg tggcgtgcag gcagcccggc
      541 tgctcggtgg cctgctgcgt ctgccgggtc agctgtgcgc cgttacggga gcgcgccgca
      601 ccggtgaaga atcggaacgc gtcaaagtgg gtgcagcgca acggcattga ctcgaccacc
      661 gcgtcggtgc ccgcactgct taatcgcagc ggcacggcgt gatgccgcac ctcggcgggc
      721 gcggcgcggt acaccatcgc ccactcgtgc agcccgaagc agccgaagtg cgcagggcgg
      781 cccgcggtcg cgtcgagcag cccgacgacg aactcgacgg tcggacggcg ccgcgccagc
      841 agatcggggt cgacggtcac cgcgccgtcg gtccgccgat acccgctcca ccgcgcatac
      901 gcctgcgacg cagggcccgt caggctgact ccgaaccccg ggtgccagcg tgcgagctgg
      961 gccggccgga gcctgtagta cgtgaacagg aaatcgacga ccggatgcga cgtgccgtgc
     1021 ccgcggcggt ccaggtacgt gccgacgagc gcggtcacgc gctcgtggtg gctgtcgcgg
     1081 cgttgcgtcc aggaagcctc gctgagcacc tcagacacgg tgcgtcccgt cgcgcacggt
     1141 gcccacgaga tcctccacca agtcctccag cgccacgatc cccacgacgc ggtcgtggct
     1201 tccggtgact agcgcgaggt gactgttgtt gcggcgcatg cgcgagagcg catccggcag
     1261 cggcatgctc tcggacacgt cgggcagcgg gcggaccgtc gagcggtcga caactgcctc
     1321 ggggtcgtcg acggccggta gcacgtcttt gatgtgcacg taaccgacga accggccagt
     1381 ggagtcgact acggggaagc gcgagtatcc ggtctcgccc agagcccgct gaatggcctc
     1441 gacggtcggg ccgtcgctgt ccgcggcgat ttccaccatg tggacctcgt tcagcgggat
     1501 ggcgacgtcg gacaccacgc ggttgcggat ctgaagcgcc cgggacagtc gcatgtgttc
     1561 ctcgggatcc agcaggcctt ccgagcgtga ttccgcgatc atctcggaca gctcgaccat
     1621 cgagacggtg ttctcgagtt cggtcttggg ctcgatgcgc accgcgcgca gcgtcacgtg
     1681 cgcacaccag tcatagaacg cgatgacggg ccgggtggct tgcacccacc acaggtacgg
     1741 cggcaccaac aacatcgcag cggactcggg cccggcgatc gcgatgttct tcggcaccat
     1801 ctccccgagc agcacgtgca acaccacgac gatcgtgatc gcgacgaaga acgacacggt
     1861 gtgcagtaac gcatcgggca cgccgagaag atcgaacggc ttctccagca ggtgagccac
     1921 tgccggttcg ccgatgcggc ccaacaggat tgagcacacc gtgatgccga gctgggcgcc
     1981 ggccagcatc agcgacaggt tctgcccggc gcggataacc gtcacggcgc tgcgcttgcc
     2041 ctgctcggcg agggcttcca gccggtcgcg ccgcgcggat atcagcgcga actcggcccc
     2101 gacgaagaac ccgttcgcga gcagcagcag gacggtcagc acgacgccga ggaagtcagc
     2161 catcagtcac cacccgaatc gccttggcgt cggccgagtt tgacgagatc cagcagatcg
     2221 atgcggcggc cggccatccg ggcgacggtc gcacgccacc ggatcgggtc gtccagagtc
     2281 ccttccggct cgaatgcctt gagctccacc gactctccga cctcggggat atgccccagt
     2341 tcatgcatca ccaacccgcc gatggtctcg tattcgccct cgtgaccgcg gaatcccgtg
     2401 gccgcggcaa cctcgtcgat acgcagcagc cccgacaccc gccagccatc gcccgaacga
     2461 aacacatcgg gcgtcggctc gtcgtgttcg tcgcggatgt cgccgacgat ctcctcgatc
     2521 aggtcttcga cggtgaccat ccccgcgatg ccgccgtatt cgtcggcgac cagcgccgtc
     2581 tgcaggccgt tggcccgaat ctgcgccatc agcgagtcgc cgtccagggt ggacggcacc
     2641 accgccaccg ggcgcgccac gtcggccagc ctggtgcggg cgaagtcgtc gtgcggcagc
     2701 tcgaaaacct gcttgacgtg gaccagaccg atcgtctcgt caagatcgcc ctcggttacc
     2761 gggaagcggg agtagccggt ctgcatcgcc ttggcgacca ggtcggcgac cgtgtcgtcg
     2821 gcctccagcg actcgatctt ggaccgcggg gtcatgaact cctcggcggt gcgcgcgccg
     2881 aactgcagcg accggtcgac cagcgccgcc gtgagcggat ccagcgcgcc gcgccgcgcg
     2941 gagttgcgca ccagcgaccc cagctcctgc ggcgagcgag ccgaccgcag ctcctcggcc
     3001 ggctcgatgc cgagccgccg cagtatccag ttcgcggtgc cgttggtgag gtggatcagc
     3061 ggggtgaaca gcagcgagaa caacacctgc ggccccgcca cgacgcgcgc ggtcggcagg
     3121 ggccgcgcca ccgccaggta ttgggccacc agctcgccga acaccattga caccgaggtg
     3181 gcgatcagca gggcgagcgc cagcgcggtg ccgtggatcc aatgctcggg taccgacatc
     3241 gccgtcagca caggtcgcaa cagccgcgcg atcagcggtt cggccagata accggtgata
     3301 agtgtggtga tcgagatgcc gatttgcgcg ccggacagct ggaacgacag cgtgcggtgc
     3361 gcgcgctgga ccattttgtc gcggcgcccc ccgctgcggg cgttggcgtc caccgtgctt
     3421 cgctcgagcg cggtgaggga gaactccgcg ctgacgaaca ccgcggtgcc cagcgtgagc
     3481 acgacgaagc ccaccagcgc gagcagaatc aatgtcacgc tcatcgcgcc gtggtctggt
     3541 cacggccgga ccggatctcc ggccgactag acggaggctc ggcctcggac ggcacgtgat
     3601 ccctttcatc cggctgcgca acgcgttcgc gccgcgccaa gaagcaccca tcgtatcgcg
     3661 atggggcctg ccggttcagc gtccagctca gcggcgaatg cgggcgtaca gggcctgcgc
     3721 cgaaatagcg ttccaggtcg tgattctccc cggatcacag ccctgagttc tgtttcggcg
     3781 caacggagcg gggaagctac cagccggtgg gcagcggatg tccttcggcg aagccggccg
     3841 cggactgcac gccgaccacc gccagctcgt gcagctcggc gatcgtcgac gcgccgacgt
     3901 aggtgcaggt gctgcgcagc ccggacgtga tgtggtcgag caggtcctcg acgccaccgc
     3961 gatcgggatc caggcccatc ttcgacgtcg agatgccctc ttcgaacagc gccttgcggg
     4021 cccgctcgta ggcgctgtcg gtggtggtgc gggctgctac cgcgcgcttg gaggccatcc
     4081 cgtagctctc cttgtacggc tggtcgtcgc ggtcgcgcag caggtcgccg ggcgactcgt
     4141 acgtgccggc gaaccacgag ccgatcatca cgttcgacgc accggccgcc agcgccagcg
     4201 ccacgtcgcg gggatgacgc accccaccgt cggcccacac gaatccaccc aattgcgctg
     4261 cggcagaggc acattcatgt accgccgaga actggggccg gcccacaccg gtcatcatcc
     4321 gggtggtgca catcgcgccc gggccgacgc ccaccttgac gatcgtcgct ccggcggtca
     4381 gcaactcgcg ggtgccctcg gcggagacga cgttgcctgc cgccacgggc acgcccaggc
     4441 cgagcgaggt caccgctttg atcgcgtcga tcatccgtgt ctggtgcccg tgcgcggtgt
     4501 cgatcaccag caggtcggcg cccgcctcga cgagcgcggt ggccctcgcg ccgacgtcgc
     4561 cattgatccc gaccgccgcg ccgatccgca gccggccggc gtcgtcgacc gcgggggtgt
     4621 acagccccgc gcgcagcgcg cctcgacggg tcaacacccc ggccagcgtg ccgtcgggct
     4681 ccgtcagcac ggcgacgtcg atctgcgcgt gttcgagcag gtcgaacacc tgccgcgggt
     4741 cggtgtgcgc gggcgcggtg acgaagtcgc ggaccagcac gtcacgcacc cgggtgaacc
     4801 tgtcgacgcc ggcgcagacg gcgtcggtga ccagtccgat cgggcggcct tcgaacacca
     4861 ccactgcggc cccgtgcgcg cgtttgtgga tcagcgcgca cgcgtccgac accgagtcgt
     4921 caggctccag cgtgacgggc gtatcggcca ccaggtcgcg gctcttgacg aactcgacgg
     4981 tgtgcttgac cgcggtgatc ggcaggtcct gcggcagcac cacgatgccg ccgcggcggg
     5041 ccaccgtttc ggccatccgg cgcccggcga ccgcggtcat gttggccacc accaccggga
     5101 tcgtcgtgcc ggagccgtcg atggtggaca ggtcgacgtc aaacctcgac gtcacgtccg
     5161 agcggttcgg gacgatgaag acgtcggtgt aggtcaggtc gtacgggggc gggtgaccct
     5221 ccacaaatcg catcgccacc gatcctaggc ggtggtggca ggcttcggcg cccgctgcaa
     5281 cagctcgagc cgggtgccgt ccttgacgat gaactgcgcc accaacgtca ccggcggctc
     5341 gacctcggcg accgcggcga aatcgtcacc gccggagcgc ctttcctgca cttcgaagcc
     5401 gagcccttca gcgtagaacc gctgagaccg gtcgagatcc gacacgcaga ggccgacgtg
     5461 agccagcgcc gtcacgcctc cacctcgctg cggtcaccgc tccacagcgt gtggaacttc
     5521 ttgccgcgct cggcgtcgac gcgttcgtag gtgtgcgcgc cgaagaagtc gcgctgggcc
     5581 tgggtcaacg cggccgggag ccgctcggtg cgcagcgcgt cgtagtacga cagcgccgac
     5641 gcgaagccgg ggatcgggat gccgagccgg gtggcggtgg cgacgacgcg ccgccagctg
     5701 tcgatgcccg cctcgaccgc gctgcggaag tacggggcgg cgatcagcgt cgccaggtcg
     5761 gggtcgtcgt cgaacgcgtc cttgatgcgg ttgaggaact tggcccggat gatgcagccg
     5821 ccgcgccaga tcgtggccag gtcaccgggt gtgacgcccc agtcgtactc cgcgctgccg
     5881 gcctggatct gattgaaccc ttgggcgtac gcgacgatct tcgacgcgta cagcgcaaga
     5941 cgcacgtctt cggtgaattc gtctgcatca cttggctttc cgccgagtga gccggacgcc
     6001 aggcccgtcg tggcggtgcg ctgggcaacc gagccggaca gcgcgcgggc gaacacggcc
     6061 tccgcgatgc cggtgacggg cacgccgaga tcgagcgcgg acttcacggt ccagcggccg
     6121 gtgcccttct gttccgcctc gtcgaggatc acgtcgacca gcggcttgcc ggtcttggcg
     6181 tcggtctggc gcagcacttc ggcggtgatc tcgatcaggt agctgtccag gtcgcccttg
     6241 ttccactcgg cgaacacgtc ggcgatctgc ggcgccgaca gcccgagtcc gtcgcgcagc
     6301 agctggtacg cctcgccgat gagctgcatg tcggagtact cgatgccgtt gtgcaccatc
     6361 ttgacgaagt ggccggaccc gtcgggcccg atgtgcgtgc agcagggcac cccgtcgacg
     6421 tgcgcggaga tctcctccaa cagcggaccc agcgacttgt acgactcggc ggggccgccc
     6481 ggcatgatcg acgggccgtt cagcgcgccc tcttcgccgc cggagatgcc ggcgcccacg
     6541 aagtgcaggc cgcgctcggc catcgccttc tcgcggcgga tggtgtcggt gtagagcgcg
     6601 ttgccgccgt cgatgatgat gtcgcccttg tccatcacgc cggcgagttc gtcgatgacg
     6661 gcgtcggtcg ggtcgccggc cttgaccatg atcagcaccc ggcgcggttt ctccagcgcc
     6721 gcgacgaatt cggccatcgt ctcggtgcgg acgaacttgc cctcggagcc gtagtgcgct
     6781 agcagggcat cggttttggc gatcgagcga ttgtgcagtg cgacggtgta gccgtggcgc
     6841 gcgaagttgc gggcgatgtt ggagcccatc acggccagcc cggtaacgcc gatctgcgcg
     6901 gtgccggtgg tgtctgcttc aggaggcgtc atcgcaaggc ctttcgtgtg cggttgtggc
     6961 gcaacgggaa atctgcccaa aaagaccaga ctatgcggtg aagagccgat gcaactcggt
     7021 gagccacggc accgccaacg ctaccgtcgg cacgaccaag atcgccgcgg cggccaggta
     7081 cgcggtggtg gccactgcca ggctgttgcc gcggcccgaa agcctccgca cgcgcgccac
     7141 cgtgctgggc ccgccggcag ccagcgcgcc cgacggcgtc ggcccggccg cgcacgccac
     7201 cagtgcgcgc gccaggggag tagggcccgc cacgcgcacc gcggcgtcgt cggcgagcat
     7261 ttcgaccaat agccgcacgg cgtccagcgc ccgggcgctg cggacgaacc tcgggaacgc
     7321 cgcgtgcacc gcggtgaaca tctcgagcac caggtcgtga cgggcccgaa gatgggcccg
     7381 ctcgtggcgc aggatcgcgg tgatctcttt gtcgctcaac gtgttcagcg cgcccttgct
     7441 cacgacgaca cggctgcgaa cgccgggcag gcaataggcc agcggctcgg agacgtcgat
     7501 gatgcgcaga tgccccgtgc ctgcggcccc gagaaggtcg acaagcatgc ggtggtgagc
     7561 tcg
//
LOCUS       DASWBF010000380         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_695125,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000380 DASWBF010000000
VERSION     DASWBF010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695125"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VF874_14145"
     CDS             complement(<1..1023)
                     /locus_tag="VF874_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9177161.1"
                     /translation="MPGPPACRITVTAADRHRLKQRARGHKTPFRDRVRALIVLLAAR
                     GIPTAVIAKRAGVSVDTARSWRNRFAATGMNGLTDRPRSGRPRRFTELQRAEVKALAC
                     ELPATRGVPLAKWSCLDLALEVVRAGITDTISASTVRRILTHDAIKPWQHRSWISVRD
                     PDFAGKAKRVLDLYARTWQGQPLGADDYVISCDEKTSIQARCRCHPTLPPGRSRTMRV
                     EHDYDRGGALAYLTAYDVHRAHVIGCCAPTTGITAFMALVAQVMTQEPYPSARRVFWV
                     VDNGSSHRGDTAAQRLADAFPTAVMVHTPIHASWLNQVEIYYSIVQRKALSPNDFTDL
                     AELENRL"
     gene            1121..1432
                     /locus_tag="VF874_14150"
     CDS             1121..1432
                     /locus_tag="VF874_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177162.1"
                     /translation="MPIPPESTKASLQYRLCEHARDRWSNLDLDVRHRAGFAYVDARL
                     ASSEVVPLLRLRYGGSASYWGVALYRAGTGKYEDQIWFTGTPVEALDFAAGILLAGAD
                     A"
BASE COUNT          262 a          541 c          614 g          297 t
ORIGIN      
        1 gagccggttc tcgagttctg cgaggtcggt gaagtcgttc ggggatagag ccttgcgctg
       61 gacgatcgag tagtagatct ccacctggtt cagccacgac gcgtggatcg gggtgtgcac
      121 catcactgcg gtggggaacg cgtcggcgag ccgttgggcg gcggtgtcgc cgcggtggga
      181 ggagccgttg tcgacgaccc agaacactcg tcgcgcggag gggtagggct cctgcgtcat
      241 gacctgggcg accagggcca tgaacgcggt gattccggtg gtgggtgcgc agcagccgat
      301 gacgtgggcg cggtgcacgt cgtaggcggt caggtaggcc agcgcgccgc cgcggtcgta
      361 gtcgtgttcg acccgcatgg tgcgggaccg gcccggcggc agcgtggggt ggcagcggca
      421 gcgggcctgg atggaggtct tctcgtcgca ggagatcacg tagtcgtcgg caccgagtgg
      481 ctggccctgc caggtgcggg cgtacaggtc aaggacgcgt ttggccttgc cggcgaagtc
      541 ggggtcgcgg accgagatcc aggaccggtg ttgccagggc ttgatcgcgt cgtgggtgag
      601 gatgcgccgc acggtggacg ccgagatcgt gtcggtgatg ccggcgcgga ccacttcgag
      661 ggcgaggtcc aggcagctcc acttcgccag gggcaccccc cgggtggcgg gcagttcgca
      721 ggccaacgct ttgacctcgg cccgttgcag ctcggtgaat cgtcgtggtc gacccgatcg
      781 gggccggtcg gtcaggccgt tcatgccggt ggcggcgaag cgattgcgcc agctgcgggc
      841 ggtgtcgacg ctgaccccgg cccgcttggc gatgaccgcg gtggggatcc cgcgcgcggc
      901 caacagcacg atcaacgcgc ggacccggtc ccggaacggg gtcttgtgcc cgcgggcgcg
      961 ctgcttcagt cggtgccggt cggctgcagt cacggtgatt cggcaggcgg gcggaccggg
     1021 catcggcaac gacccttttc gagattgacg gcgggagccg aaacctctcg agaaatggat
     1081 cttggttcaa tctgacacgc cgatcgggca gcatggccgc atgccgatcc cgccggaatc
     1141 caccaaagct tcgctgcagt accggctgtg cgagcacgcg cgcgaccgct ggtccaacct
     1201 tgaccttgac gtccgccacc gcgctgggtt cgcctacgtc gacgcccgcc tcgccagcag
     1261 cgaggtcgtt ccgctgctgc ggctgcgcta cggcggctcg gcctcctact ggggagttgc
     1321 cctgtaccgc gccggcaccg gaaagtacga agatcagatc tggttcacag gcacgccggt
     1381 ggaggcactc gatttcgccg ccggcatctt gctggccggc gccgatgcct gatcagtgcc
     1441 cccggctgac ccggtgctcg cgcaacgcca accaactggc gacggactta cggcgtcgac
     1501 cactaagctc tagcgactga ggggactccg gcactctctc ccagcgcagt accgctgcag
     1561 tcagcgaccg catcagttct tctcgtccct gcgtaagcgc accctgcggc agcaccaatt
     1621 cgaccatcgg cattgagacc tcctttgtgg atcgttgtaa ttgtcgcgct gtcccggaag
     1681 agagcgtaaa cacaatgcgt cagactaact cctt
//
LOCUS       DASWBF010000381         3612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_246659,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000381 DASWBF010000000
VERSION     DASWBF010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246659"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VF874_14155"
     CDS             <1..656
                     /locus_tag="VF874_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX9177163.1"
                     /translation="QFHHPLQLVWFAVIGIIAAAVGLLYAKTFYGTVGLTARLPGGTV
                     IKPALGGLCVGLLALMLPQVLSSGYGWAQKAAATETLMAIPLWIVLILPVAKIVATSL
                     SIGTGGAGGIFGPGIVIGGFTGAAIWRLGELVHAPGLPQSPGVFVVVGMMACFGSVAH
                     APLAVMIMVAEMTASFSVVPCAMVAVGIAYLLISRTDVSIYKAQRLNRETAHEERLE"
     gene            678..1724
                     /locus_tag="VF874_14160"
     CDS             678..1724
                     /locus_tag="VF874_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9177164.1"
                     /translation="MTTAHSPHATSLTNGDVVEQSELGSITRVTSDTFPILRGLSIKR
                     VVINPGAMRTPHWHANANELTYCVSGTALVSVLDTGSEFSSFVVSAGDMFHIDSGSLH
                     HIENIGTDTAEFVIAFRHERPEDFGLAASFGAMTDAVLGNTYDLPASDFATLRRDTTD
                     RALAARDGDPEVPATAYFSDPHKFSVEASAAPISIAVGSAHVARVQFWPALKDLAMYS
                     LRIREDGMREPHWHPITAEMGYVHRGSARMTVMDPDGTVDTWYLQEGDVYFIPRAYPH
                     HIEVFESDDIHFLVFFDQPTPGDIGYRASVSAYSRQVLAATFNTHLAALPVFPFTPED
                     PLIVTRCNPVDPRA"
     gene            complement(1728..2273)
                     /locus_tag="VF874_14165"
     CDS             complement(1728..2273)
                     /locus_tag="VF874_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX9177165.1"
                     /translation="MAQFTVPGMSEKQGSEIAELLQKQLSRYNDLHLTLKHVHWNVVG
                     PNFIGVHEMIDPQVSLVRSYADEVAERIAALGKSPKGTPGAIINDRTWDDYSVDRDTV
                     QAHLAALDLVYNGVIEDTRKGIDRLEDLDLVTQDMLIEHAGEMEKFQWFVRAHLENAG
                     GSLKHGGKKTEKSAARAAKSA"
     gene            complement(2359..3270)
                     /locus_tag="VF874_14170"
     CDS             complement(2359..3270)
                     /locus_tag="VF874_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX9177166.1"
                     /translation="MKSERSISFPGPARPLATLSPLRRGRGDPCFLIAADGAIWRTSL
                     MASGPVTARITKSAPETVECEAWGDGAAEFVDAAPGMLGADDDATGFAPEEPTIAAAH
                     RKAPHLRLGRTGRVLEALIPAVVEQRVAGADAFRSWRLLVTRYGAPAPGPAPARMRVP
                     PSAEVWRRIPSWEFHRANVDPGRARTIVGCAQRASSLERLTQRAPESARAALTSLPGV
                     GVWTAAETAQRAFGDADALSIGDYHLATVIGRTLLGHPIDDAQMVALLEPLRPHRYRA
                     VRLLEVSGLASYARFGPRQAIKNLSKL"
BASE COUNT          550 a         1234 c         1207 g          621 t
ORIGIN      
        1 tccagttcca ccatccgctg cagctggtct ggttcgccgt catcggcatc atcgctgccg
       61 ccgtcggact gctgtacgcc aagacgttct acggcaccgt cggcctgacg gcgcggctgc
      121 cgggcggcac ggtgatcaag ccggcgctcg gcgggctgtg cgtcggcctg ctggcgctga
      181 tgcttccgca ggtgctgtcc agcgggtacg gctgggcgca gaaagccgcg gccaccgaga
      241 cgctgatggc gatcccgctg tggatcgtgc tgatcctgcc ggtggccaag atcgtcgcca
      301 cgtcgctgtc gatcggcacc ggcggcgcag gcggcatctt cgggccgggc atcgtgatcg
      361 gcgggttcac cggggcggcg atctggcggc tcggcgagtt ggtgcacgcg ccggggctgc
      421 cgcagagccc gggtgtgttc gtcgtcgtcg gcatgatggc gtgcttcggc agcgtcgccc
      481 acgccccact cgcggtgatg atcatggtcg ccgagatgac ggcgtcgttt tccgtggtgc
      541 cgtgcgcgat ggtcgcggtc ggcatcgcgt acctgctgat ctctcgcacc gacgtgtcga
      601 tctacaaggc gcagcggctc aaccgggaga ccgcgcacga agagcggctc gaatagcaga
      661 cgaagaaagg cacagccatg acaaccgcgc acagcccaca cgcgacctcg ctgacgaacg
      721 gcgatgtcgt cgagcaatcc gaactcggct cgatcacccg ggtgacctcc gacacgttcc
      781 cgatcctgcg tgggctgtcg atcaagcggg tggtcatcaa ccccggggcg atgcgcacgc
      841 cgcactggca cgccaacgcc aacgaactca cctactgcgt gtcgggcacg gcgctggtct
      901 cggtgctcga caccggcagc gagttctcct cgttcgtcgt cagcgcgggc gacatgttcc
      961 atatcgactc cggctcactg catcacatcg agaacatcgg caccgacacc gcggagttcg
     1021 tcatcgcatt ccgtcacgag cgtcccgagg acttcggtct cgccgcgtcg ttcggcgcga
     1081 tgaccgacgc cgtgctgggc aatacctacg acctgccggc gtccgacttc gccacgctgc
     1141 gccgcgacac caccgaccgg gcgctcgccg cccgcgacgg cgaccccgag gtgccggcca
     1201 ccgcgtactt ctcggacccg cacaaattct ccgtcgaagc gagcgcggcg cccatcagca
     1261 tcgcggtcgg ttcggcgcat gtggcgcggg tgcagttctg gccggcgctc aaagatctgg
     1321 cgatgtactc gctgcgtatc cgcgaggacg ggatgcgcga accacactgg cacccgatca
     1381 ccgccgaaat gggctacgtg caccgcggct cggcgcggat gaccgtgatg gatccggacg
     1441 gcaccgtcga cacctggtac ctgcaggagg gtgacgtcta cttcatcccg agggcgtatc
     1501 cgcaccacat cgaggtcttc gagtccgacg acatccactt tttggtgttc ttcgaccagc
     1561 cgacacccgg cgacatcggc taccgcgcgt cggtcagcgc ctattcacgg caggtgctcg
     1621 ccgcgacgtt caacacgcat ctggcggcgc tgccggtgtt tccgttcacg ccggaggatc
     1681 cgctgatcgt gacccgctgc aacccggtcg acccgcgcgc ctgagggtca ggcgctcttc
     1741 gcggctcggg cggcactctt ctcggtcttc ttgcccccat gcttcagcga tccgcccgcg
     1801 ttctcgagat gcgcgcggac gaaccactgg aacttctcca tctcgccggc gtgctcgatc
     1861 agcatgtcct gggtgaccag gtcgagatct tcgagccggt cgatgccctt gcgggtgtct
     1921 tcgatcacgc cgttgtagac caggtcgagg gcggccagat gcgcctggac ggtgtcccgg
     1981 tcgaccgagt agtcgtccca cgtgcggtcg ttgatgatcg cgcccggtgt gcccttcggt
     2041 gacttaccga gtgccgcaat gcgttccgcg acctcgtcgg cgtagctgcg caccagcgac
     2101 acctgcgggt cgatcatctc gtggacgccg atgaagttgg gaccgaccac attccagtgg
     2161 acgtgcttga gcgtcaggtg cagatcgttg taccggctca gctgcttctg cagcagctcg
     2221 gcgatctcgc tgccctgctt ctccgacatg cctggaacgg tgaactgcgc cattgctaac
     2281 tcctcgtacg tacccgtttt ctggattcgg tcaagcagag gggtacccat gcggacgcgc
     2341 gcgtaatcga gacgaccatt acagcttcga aagattcttg atcgcctgcc gcggcccgaa
     2401 ccgcgcatag ctggccagtc cgctgacctc gagcagccgc accgcccgat agcggtgcgg
     2461 gcgcagcggc tccaacaacg cgaccatctg cgcgtcgtcg atcggatgtc cgagcagcgt
     2521 ccggccgatc accgtggcca agtggtagtc gccgatcgac agcgcatcgg catcaccgaa
     2581 cgcgcgctgc gcggtctcgg cggcggtcca caccccgacg cccggcagcg aggtcagcgc
     2641 ggcgcgggcc gactccggtg cccgctgggt cagccgctcc agcgacgacg cccgctgcgc
     2701 gcagccgacg atcgtgcgcg ctcggccggg gtcgacgttg gcgcggtgga actcccacga
     2761 cgggatgcgc cgccacacct ccgccgacgg cggcacccgc atccgggccg gcgcgggccc
     2821 gggcgcgggc gcgccgtacc tggtcaccag cagccgccac gaccgaaacg cgtcagcgcc
     2881 agccacccgc tgctccacca ccgcggggat cagcgcctcg agtacccgcc cggtccgtcc
     2941 gagacgcaga tgcggcgcct tgcggtgggc cgccgcgatc gtcggctcct ccggcgcgaa
     3001 cccggtcgca tcgtcgtcgg cacccaacat ccccggcgcc gcgtcgacga actcggcggc
     3061 gccgtcgccc caggcctcac actcgactgt ttctggcgcc gacttggtga tccgggccgt
     3121 caccggaccg ctggccatca ggctggtccg ccagatcgcc ccgtcagctg cgatcagaaa
     3181 gcacgggtcg ccgcggccgc gccggagcgg ggacagcgtc gccagcggtc gcgccggccc
     3241 gggaaacgag atgctgcgtt cggatttgat gacccaatcg tgacatccgt ccgccgggtg
     3301 agcgatgggt ggcgggctcg gatgtaatgg gcctgagttg ttctgaaatg tgacgggtta
     3361 agagttgttg ggcgaaatgc gcgtgatcgg gtgtttgtga gcattggtga gtgtgtgacg
     3421 atgttgtcgg tgcggtctgg gatggttgag tcatgtgccg cggatcggcg aagttgccat
     3481 tggggcgtgg tgatggcgcg gtttgaggtt cccgacggct gggtgagggc acgaggcgac
     3541 gaagcgtcaa ccccgcccac cactcacgag ggtgcgggga ccgctgctcc gcaaggagaa
     3601 gctgcctgaa at
//
LOCUS       DASWBF010000382         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_430242,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000382 DASWBF010000000
VERSION     DASWBF010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430242"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF874_14175"
     CDS             <1..663
                     /locus_tag="VF874_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9177167.1"
                     /translation="FGNGVLGSLQRHADPNAVHVLLSHLHADHCIELPALFVWRRYHP
                     IPAVGKALMYGPSDTWSRLAQASSPFGGEVDDFSDIFDVRHWNDGEEVEFGSVRVLPR
                     AVAHPTEAYGLRFTDASGAVFTFSGDTGMCDAIIDLAQGADVFLCEASWTHDPGHRPE
                     HLHLSGTEAGQIAARAGVGELLLTHIPPWTSREDIITEAKAAFDGPVRAVVCQEAFEV
                     TH"
     gene            664..1443
                     /gene="rph"
                     /locus_tag="VF874_14180"
     CDS             664..1443
                     /gene="rph"
                     /locus_tag="VF874_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX9177168.1"
                     /translation="MSSREDGRLDDELRPVTITRGFTSHPAGSVLVEFGETRVMCTAS
                     VTEGVPRWRKGTGEGWVTAEYAMLPAATHTRSDRESVRGRLGGRTQEISRLIGRSLRA
                     CIDLAALGENTIALDCDVLQADGGTRTAAITGAYVALADAVTYLSAAGRLSDPRPLSC
                     MIAAVSVGVVDGRVRVDLPYEEDSRAEVDMNVVATDTGTLVEIQGTGEGATFPRSTLD
                     KLLDAALGATEKLFEIQRAALELPYPGELPEGPPAKKAFGS"
     gene            1445..2044
                     /gene="rdgB"
                     /locus_tag="VF874_14185"
     CDS             1445..2044
                     /gene="rdgB"
                     /locus_tag="VF874_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX9177169.1"
                     /translation="MIRLLVASRNRKKLAEMRRVLDSAGLAFELVSLSDVPPFDEAPE
                     TGATFEDNALAKARDAFKATGLPSVADDSGLEVDALHGMPGVLSARWAGVHGDDAANN
                     ALLLAQLRDVPSNRCGAAFVSTCALVSDADEVVVRGRWPGRIAREPRGESGFGYDPIF
                     LPDGLECSAAELSPEQKDSMSHRGRALAQLVPALRALAV"
     gene            complement(2052..2906)
                     /locus_tag="VF874_14190"
     CDS             complement(2052..2906)
                     /locus_tag="VF874_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177170.1"
                     /translation="MALAGGGLLATAVVIAPFVISAPINAWHDVVVAQFSRPQNPFVP
                     DGLDRLIDMVGLGAVGIPVALVLIALLSIATVPYFSPQVGLWVSVLIIIGCGFATSPT
                     YFTHYGESLGPAVALLVSRSLDIRRGALLVTAFAVAFAIGTGVHLSGLRGQGDLRAAT
                     AEIPDGSCVYFDAISLPLMADVYTVPNPRCPSYVDGRGVALAQNIKWDNRVSFYPSGF
                     VADKRWQDATIEQMKHADYLLLRSDPATFPEWDESTRAYALSHFTLAVRNPDGRQPFE
                     LWKRTAPA"
BASE COUNT          496 a          885 c         1059 g          492 t
ORIGIN      
        1 tttggtaacg gcgtgctggg gtcgctgcag cgccacgccg acccgaacgc ggtacacgtc
       61 cttctgtctc atctgcatgc cgaccactgc atcgaactgc ccgcgctttt cgtgtggcgc
      121 cgctaccacc cgatcccggc cgtcggtaag gcgttgatgt acgggcccag cgacacctgg
      181 tcgcggctgg cgcaggcgtc ctctccattc ggcggcgagg tcgacgactt ctccgacatc
      241 ttcgacgtgc ggcactggaa cgacggcgag gaggttgaat tcggatcggt gcgggtgctg
      301 ccgcgagcgg tggcccatcc gaccgaggct tacgggctgc ggttcaccga cgcatccggt
      361 gcggtattca ccttcagcgg cgacaccggc atgtgtgacg cgatcatcga tctggcgcag
      421 ggcgccgacg tgttcctgtg cgaggcgtca tggacgcatg accccggcca ccggcccgag
      481 catctacatc tgtcgggcac cgaggcaggc cagatcgccg cccgcgccgg agtcggggag
      541 ttgctgctga cgcacatccc gccgtggacg tcgcgcgagg acatcatcac cgaagccaaa
      601 gcggcgtttg acgggccggt gcgggccgtg gtgtgccaag aggcgttcga ggtcacccac
      661 tagatgtcca gtcgagagga cggccgcctt gacgacgagc tgaggccggt gaccatcacc
      721 cgcggattca cgtcccatcc ggccggctcg gtgctggtgg agttcgggga aaccagggtg
      781 atgtgcacgg ccagcgtgac cgagggagtt ccgcggtggc gcaagggaac tggcgaaggc
      841 tgggtgaccg cggagtacgc gatgctgccg gcggccacac acacccgatc ggaccgcgaa
      901 tcagtgcggg gccgcctcgg cggacggacg caggagataa gccggttgat cggacggtca
      961 ctgcgggctt gcatcgacct ggccgcgctc ggagagaaca caatcgcact cgactgcgac
     1021 gtgctgcagg ccgacggcgg cacccgcacc gcggcgatca ccggcgccta cgtcgcgctg
     1081 gctgacgcgg tcacctatct gtccgcggcc ggcaggttgt cggacccccg accgctgtca
     1141 tgcatgatcg ccgcggtgag cgtcggtgtc gtcgacggaa gggtccgggt cgatctgccc
     1201 tacgaggagg attcgcgagc cgaggtcgac atgaacgtcg tggctaccga caccggcacg
     1261 ctggtggaaa ttcaggggac cggcgagggc gcgacattcc ctcgttcgac gctggacaag
     1321 ctgctggacg cggcgctggg cgccaccgag aagctgttcg agattcagcg ggcggcgttg
     1381 gagttgccgt atccgggaga gctgcccgag ggcccgccgg cgaagaaggc gttcggcagc
     1441 tgacgtgatt cggctgctcg ttgcgagccg caaccgcaag aagttggcgg agatgcggcg
     1501 ggtgctcgac tcggccgggc ttgcgtttga gttggtgtca ctttccgacg tgccgccttt
     1561 cgatgaggca ccggagaccg gtgcgacgtt cgaagacaac gcgttggcga aggcgcgtga
     1621 cgcgttcaag gcgaccgggt tgccgtcggt ggccgacgac tccggactcg aggtcgacgc
     1681 gcttcacggc atgcccgggg tgctgtctgc ccggtgggcc ggggtgcacg gcgacgacgc
     1741 cgcgaacaac gcgctgttat tggctcagtt gcgggacgtc ccctcgaacc ggtgcggcgc
     1801 ggcgttcgtg tcgacgtgcg cgctggtgtc ggacgccgat gaggtggtgg tgcgcggacg
     1861 gtggccgggg cggatcgcac gggagccgcg cggcgagagc ggcttcggct acgacccgat
     1921 atttctcccc gacgggttgg agtgcagcgc cgccgaactc agtcctgagc agaaggactc
     1981 gatgtcgcat cggggccgag cgctggctca gctggtgccg gcgctgcggg cgcttgcggt
     2041 ttgaactcgc gtcacgcggg cgccgtgcgc ttccacagct caaatggctg ccggccatct
     2101 ggattccgga cggcgagagt gaagtggctc aatgcgtaag cgcgggttga ctcgtcccat
     2161 tccgggaacg tcgccgggtc acttcgcagg agcaggtagt cggcgtgctt catttgttcg
     2221 atcgtggcgt cctgccaacg tttgtcggcg acgaacccgg agggatagaa gctgactcta
     2281 ttgtcccatt tgatgttctg tgctagagcg acgccgcgtc cgtcgacgta ggacgggcag
     2341 cgagggttgg gcacggtgta gacatcggcc atgagcggaa ggctgatcgc gtcgaagtag
     2401 acgcaactgc cgtcgggaat ttcagcggtg gcggcgcgca ggtcgccttg gccccgaagg
     2461 ccgctgaggt ggacgccggt tccgatcgca aatgcgacag caaacgctgt gacgagcagt
     2521 gctccccgtc gaatatcgag tgatcggctc acgaggagag cgacagcggg accgagggat
     2581 tcgccatagt gcgtgaagta tgtcggcgag gttgcgaatc cacagccgat aattatgagc
     2641 actgacaccc acaggccgac ttgcggactg aaatacggca ccgtcgctat ggagagtaaa
     2701 gcgatcagta cgagggcaac cgggatcccc acggccccga gcccgaccat gtcgatgagg
     2761 cgatcgagac cgtccggcac gaatgggttt tgcggccggc tgaactgggc gacgacgacg
     2821 tcgtgccagg cgttgattgg agccgagatg acaaatggtg cgatgacgac agccgtagcg
     2881 agcagcccgc cgcccgcgag agccaagagc gactttggtc ggcgtgtcca ca
//
LOCUS       DASWBF010000383         5801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_510552,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000383 DASWBF010000000
VERSION     DASWBF010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510552"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /gene="marP"
                     /locus_tag="VF874_14195"
     CDS             <1..570
                     /gene="marP"
                     /locus_tag="VF874_14195"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance serine protease MarP"
                     /protein_id="HEX9177171.1"
                     /translation="RGLANGCQKVLEGTGFVVKPGRVMSNAHVVAGADSVTVESDGTT
                     YDAKVVSYDPNADISILEVPDLQAPPLSFVDGDVKPGTDAVVLGYPGGGEFVATPARI
                     RETIELNGPDIYRQTTVTRKVYTIRGTVRQGDSGGPLIDLDGRVLGVVFGAAVDDPDT
                     GFVLTADEVHRQMEKVNDTEPVPTGPCIT"
     gene            complement(563..1525)
                     /locus_tag="VF874_14200"
     CDS             complement(563..1525)
                     /locus_tag="VF874_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9177172.1"
                     /translation="MPPPPPSVVRIDGPWRHMEVHANGIRFHVVEAEPPGQAGEPVSA
                     RPLVVLLHGFGSFWWSWRHQLTGLRGVRVVAVDLRGYGGSDKPPRGYDGWTLAGDTAG
                     LIRALGHSSATLVGHADGGLVCWATSMLHPRLVRAIAIVSSPHPAALRASALTRRDQA
                     RALLPSLLRYQVPMWPERWLTRHDAEALEELVRSRATAKWVESEDFSVSMGHMRRAIR
                     IPGAAHCALEYQRWAVRSQLRTEGHRFMRSMDRQLGVPLLHLRGDADPYVLADPVHRT
                     ERYAPHGRFVSVSGAGHFSHEEAPEMVNEQLARFLGNVYGPTLR"
     gene            complement(1525..2049)
                     /locus_tag="VF874_14205"
     CDS             complement(1525..2049)
                     /locus_tag="VF874_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX9177173.1"
                     /translation="MTNGDRNGVHRKGGVPNTITSIPLTDPHALPANPSIGELVKDAS
                     SQVSSLVRAEVELARAEITRDVKRGLTGSVFFIAALVVLFYSTFFFFFFIATLLDEWL
                     PRWASYLIVFGIMLVTTALLALLGYLKVRRIRGPRETIESVKETREVFTSHDQKPAVQ
                     ANGNPPPASGPSGW"
     gene            2251..2886
                     /locus_tag="VF874_14210"
     CDS             2251..2886
                     /locus_tag="VF874_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HEX9177174.1"
                     /translation="MSAPAVAAAADKVPLGGGAGIVVDGATLCTLTAIGTDNQGSLVG
                     FTSAHCGGPGAEVAAEGAEGAGTLGVMVAGNDALDYAVIKFDPAKVTPVNTYKGFAIT
                     GLGPDPSFGQIACKLGRSTGYSCGVSWGPGEEPGTLLSQVCGGPGDSGAPVTVNNQLV
                     GMIHGAFSEKLPGCISKYIPLHTPAVAESITAILADLSAKNRPGAGFVPIT"
     gene            complement(2885..3243)
                     /locus_tag="VF874_14215"
                     /pseudo
     CDS             complement(2885..3243)
                     /locus_tag="VF874_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925207.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
     gene            3296..4891
                     /locus_tag="VF874_14220"
     CDS             3296..4891
                     /locus_tag="VF874_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9177175.1"
                     /translation="MSRMRVVVAILLLLAAVAGCGGGSSTSDVVTVNGGEPQNPLIPT
                     NTNETNGGRIVDRLFAGLMSYDNNGNPSLEVAQAVDTSDNINYRITLKPGWKFTDGSP
                     VTARSFVDAWNYGALTTNAQLQQSFFSPIDGYDAVAAETPTAQTMSGLRVVSDTEFTV
                     KLKAPTIDFKLRLGFTPFYPLPDVAFKDMAAFGQWPIGNGPYKLADGDAWQHNVKVDL
                     MPNPDYKGNRVPKNTGLRFVFYANLDTAYADLLAGNLDVLDTVPPSALPTYRNDLGDR
                     AVTGPVAINQTLDTPLRLPHFGGEEGRLRRLAISAAINRPQICKQIFTDTRSPARDFT
                     AKSLPGYDPDIPGNDALNFDPARAKQLWAQANVISPWSGRYVIAYNADGGHQEWVDAV
                     ANSIKNTLGIDAAGVPQPTFAQIRTQITDRSIGTAFRAGWQGDYPSLLEFLEPLFVTN
                     AGSNDVDYSNPQFDQALIRAQAAPTLPESYRLTNEAQQILLRDMPVVPLWDYINAAGH
                     SPAVRDVTITWNGLPGYEGIVKG"
     gene            4896..>5801
                     /locus_tag="VF874_14225"
     CDS             4896..>5801
                     /locus_tag="VF874_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9177176.1"
                     /translation="MAAYIGRRILAMIPVFLGATLLIYAMVFLLPGDPIAALGGDRPV
                     SPALAAQLRAQYHLDEPFWLQYLKYLQGLTQGDLGRSFSGLPVKDVLAQAFPVTIRLA
                     LVALAVEAVFGIGFGVVAGLRKGGLFDATVLVTSLVIIAVPIFVLGFLAQFVFGVKLG
                     IAPVTVGDRATFGRLLLPGFVLGAVSFAYVVRLTRSAVSENAGADYVRTATAKGLSRP
                     RVVTVHVLRNSLIPVVTFLGVDVGALMAGAIVTEGIFNIHGVGGALYQAITRQEAPTV
                     VSIVTVLVVVYLLANLLVDVLYAALD"
BASE COUNT          975 a         1941 c         1908 g          977 t
ORIGIN      
        1 cgcggcttgg ccaacggctg ccagaaggtc ctcgaaggca ccggcttcgt cgtcaaaccg
       61 ggccgggtga tgtccaacgc tcacgtggtc gcgggagccg acagcgtcac ggtggaatcc
      121 gacggcacga cgtatgacgc gaaagtggtt tcctacgacc ccaacgccga catctcgata
      181 ctcgaggtgc cggatctgca agcgccgccg ctgtcgttcg tcgacggcga cgtcaagcct
      241 ggcaccgacg cagtggtact ggggtatccc ggcggcggcg agttcgtggc caccccggca
      301 cgcatccgtg agaccatcga actcaacgga cctgacattt atcggcaaac gacggttacc
      361 cgcaaggtgt acacgatcag aggcactgtg cggcaagggg attcgggagg accgctgatt
      421 gacctcgacg gccgggtgct cggcgtggtg ttcggtgcgg ccgtcgacga tcccgacact
      481 ggcttcgtcc tgaccgccga tgaagtgcat cgccagatgg agaaggtgaa cgacaccgaa
      541 ccggtaccca ccggtccgtg tatcacctga gagtcggtcc gtacacgttg ccgaggaatc
      601 gcgctagctg ttcgttgacc atctccggcg cctcttcgtg actgaaatgc cctgcgcctg
      661 agacggatac aaatcggccg tggggcgcgt agcgttccgt gcggtgcacg gggtcggcga
      721 gcacatacgg gtcggcgtca ccgcgaaggt gcagcagcgg aaccccgagt tggcgatcca
      781 tcgaacgcat gaagcggtgg ccctcagtgc gcagttggct gcgcaccgcc cagcgctggt
      841 actccagcgc gcaatgcgcg gcacccggaa tccgtatcgc gcgccgcatg tggcccatcg
      901 agaccgaaaa atcctcagac tcaacccatt tcgcagtggc gcgagaacga acaagctctt
      961 cgagtgcttc ggcgtcgtgc cgggtcagcc agcgctcggg ccacatcggc acttgatagc
     1021 gcagcagcga cggcagcaac gcccgggcct ggtctcggcg ggtgagcgca gaagcgcgca
     1081 gcgccgcggg atgcggcgag ctgactatcg ctatcgcccg caccagccgc ggatgcagca
     1141 tcgacgtcgc ccagcagacc agcccgccgt cggcgtgacc gaccagtgtg gccgacgagt
     1201 ggcccaacgc ccgaatcagc ccggccgtgt caccggccag tgtccatccg tcgtagccgc
     1261 gcggcggctt gtcgctgccg ccgtagccgc ggaggtcgac ggcgacgacg cggacgccgc
     1321 gcagtcccgt cagctgatgg cgccacgacc accagaacga gccgaacccg tgcagcagaa
     1381 ccaccagcgg ccgtgcggat accggctcgc cggcctgccc gggtggctcg gcctcgacga
     1441 cgtggaaccg gatcccgttg gcgtgcacct ccatatgccg ccacggcccg tcgattcgga
     1501 cgaccgacgg agggggtggc ggcactacca acccgagggg ccggaggcag gcggcgggtt
     1561 tccgttggcc tggaccgcgg gcttctggtc gtgggacgtg aacacctctc gggtttcctt
     1621 gaccgactcg atcgtctcgc ggggtccgcg gatgcgtcgc accttgaggt aaccgagcag
     1681 cgcgagcagt gcggtggtca ccaacatgat cccgaacacg atgaggtagg acgcccaacg
     1741 cggcagccac tcgtcgagca gcgtcgcgat gaagaagaag aaaaagaacg tcgagtagaa
     1801 cagcaccacc agcgcggcga tgaagaacac gctgcccgtc aggcccctct tgacgtcacg
     1861 ggtgatctcc gcgcgcgcca gctccacctc ggcgcgtacc aagctcgaca cctggctgga
     1921 ggcgtccttg accagttcgc cgatcgacgg gttggccggc agggcatgcg ggtccgtcaa
     1981 cgggatcgac gtgatggtgt tcggcactcc ccccttgcga tgaaccccgt tgcgatcacc
     2041 gttggtcacg cacgttcctt ccgctgtgtt cttgcccgaa cctcgccgac actacggttg
     2101 ttgagggatt ccggacgatt ttcgtcaaca accgtagtct cggtgaccga agtgcccgac
     2161 ggctacccgg gttctcgtcg acaggagtgt gtgttgagga tcgcgcacgg cccgcggctg
     2221 ctcgcggcgg tcctcgctgc cgggctttgg atgtccgcgc cggccgtcgc ggcggccgcc
     2281 gacaaggttc cgctcggcgg tggcgcgggc atcgtcgtcg acggcgcgac gctgtgcacg
     2341 ctgaccgcga tcggcaccga caaccagggc agcctcgtgg gattcacctc cgcgcactgc
     2401 ggcgggcccg gcgcggaggt ggccgccgag ggcgccgaag gagccgggac gctgggcgtg
     2461 atggtggccg gcaacgacgc gctggactac gccgtgatca agttcgatcc ggccaaggtg
     2521 acaccggtca acacctacaa ggggtttgcg atcaccggcc tcggaccgga cccgtcgttc
     2581 ggccagatcg cctgcaagct gggccgctcc accggctact cgtgcggtgt cagctggggt
     2641 cccggcgagg agccgggcac cctgctcagt caggtctgcg gcgggcccgg cgactcgggc
     2701 gcacccgtga cggtgaacaa ccagcttgtc ggcatgatcc acggcgcgtt cagcgagaag
     2761 ctgcccggct gcatcagcaa gtacattccg ttgcacaccc cggcagtggc cgagtcgatc
     2821 accgcgatcc tggccgacct gtcggccaag aaccgccccg gcgcgggctt cgtcccgatc
     2881 acctagccgc cttggacacc tctgcctgca actccgcgta cgtcagggtg cggctgtcgc
     2941 cgacgggttc gccctcccag tagatcgcca cccggtcgcc gtggccggcc tcgacatggc
     3001 ggtcgacgca gttgtaggcg acgttgagct tgccgccgac gaaccacttc gcgaccggcg
     3061 cctccgacca gtcgagcacg tcggtgaacg gcgtctccca ggacagccgg ttcgcctgct
     3121 ccgcccagaa cgccaatctg tcggcctcgg cgtccctgaa caggtcggcg gttgcattgg
     3181 cttgcttggc gaactcgtcc ggcggcggga agtcaccggg gcttctgcgt gcgtctcggt
     3241 catggttccg agcctaatca gcgacagcgc ggcgggtggc tctattgtct gcgcaatgag
     3301 ccgaatgcgt gtcgtggtgg cgatcctgct gctgctggcc gcggtggcag gctgcggagg
     3361 cgggagcagc acctcggacg tcgtgaccgt caacggcggt gagccgcaga acccgctgat
     3421 ccctaccaac accaacgaga ccaacggcgg tcgcatcgtc gaccgtctgt tcgccggact
     3481 gatgtcctat gacaacaacg gcaacccgtc gctggaggtg gcgcaggcgg tcgacacctc
     3541 cgacaacatc aactaccgga tcacgctgaa gccgggatgg aaattcactg acggctcgcc
     3601 ggtgacggcc cgctccttcg tagacgcgtg gaactacggc gcgttgacca ccaacgccca
     3661 actgcagcag agcttcttca gcccgatcga cgggtatgac gcggttgcag ccgaaacgcc
     3721 caccgcgcaa acgatgtcgg ggctgcgggt cgtcagcgac accgagttca cggtcaagct
     3781 caaggcgccc accatcgact tcaagttgcg gctcgggttc acgcccttct acccgctgcc
     3841 tgacgtggcg ttcaaggaca tggcggcgtt cgggcagtgg ccgatcggca acgggccgta
     3901 caagctggcc gatggggacg cgtggcagca caacgtgaaa gtcgacctga tgccgaatcc
     3961 cgactacaag ggcaaccggg ttccgaagaa caccgggttg cggttcgtgt tctacgcgaa
     4021 cctcgacacc gcgtacgcgg atctgctcgc cggcaacctc gacgttcttg acaccgtccc
     4081 gcccagcgcg ttgccgacct accgcaacga cctcggcgac cgcgcggtga ccgggccggt
     4141 ggccatcaac cagacgctcg acaccccgct gcggctaccg catttcggcg gcgaggaggg
     4201 ccggctgcgc aggcttgcga tttcggccgc gatcaaccgg ccgcaaatct gcaaacagat
     4261 cttcaccgac acccgctcac cggcaaggga tttcacggcc aagtcacttc cgggctatga
     4321 ccccgacatt cccggcaacg acgcgctgaa cttcgatccg gcacgcgcca agcagttatg
     4381 ggcccaggcc aacgtgatat ccccgtggtc cggcagatat gtgatcgcgt acaacgccga
     4441 cggcgggcat caggagtggg tggacgcggt ggccaacagc atcaagaaca ccctcggcat
     4501 cgacgccgcc ggggtgccgc agcccacctt cgcgcaaatc cgcactcaga tcaccgaccg
     4561 ctcgatcggc accgcattcc gcgccggctg gcagggcgac tacccgtcgc tgctcgaatt
     4621 cctcgagccg ctgttcgtca ccaatgccgg ttccaacgac gtcgactact ccaacccgca
     4681 gttcgaccag gcgctgatcc gggctcaggc tgcaccaacc ctgcctgagt cctaccggct
     4741 gaccaacgaa gcacagcaga tcctgttgcg cgacatgcca gttgtgccgt tgtgggacta
     4801 tattaacgcc gccggccact cgccggccgt ccgagacgtg acgatcacgt ggaacgggct
     4861 gccgggatac gaaggcatcg tcaagggcta gccgagtggc tgcatatatc ggtcgtcgca
     4921 ttctggcgat gatcccggtg tttctcggtg cgacgctgct gatctacgcg atggtgttcc
     4981 tgctgcccgg tgacccgatc gccgcgctcg gcggtgaccg gccagtgagc ccggcgctgg
     5041 ccgcgcaact gcgggcccaa taccatctcg acgagccgtt ctggctgcag tatctaaaat
     5101 atttgcaggg gctcacccag ggcgacctcg gccggtcgtt ctccgggttg ccggtcaaag
     5161 atgttctggc gcaggcgttt cccgtcacca tccggttggc gttggtggcc ttggccgtcg
     5221 aggcggtgtt cggcatcggt ttcggtgtgg tggccgggtt gcgcaagggc gggctgttcg
     5281 acgcgacggt tctggtgacg agcctggtga tcatcgcggt gccgatcttt gtgctcgggt
     5341 tcctggcgca gttcgtgttc ggcgtcaagc tcggcatcgc gccggtcacc gtcggggatc
     5401 gggcgacgtt cggccgactg ttgttgccgg gcttcgtgct cggcgcggtg tcgttcgcgt
     5461 acgtagtgag gctgacccgc tccgcggtga gcgagaacgc cggtgccgac tacgtgcgga
     5521 ccgccacggc caagggactg tcccgcccgc gggtcgtcac cgtgcacgtt ctgcgcaact
     5581 cgctgattcc cgtggtgaca ttcctgggtg tcgacgtcgg cgcgctgatg gccggcgcca
     5641 tcgtcaccga gggcatcttc aacatccacg gcgtcggcgg cgcactgtat caggcgatca
     5701 cccgccagga ggcgccgacc gtggtgtcga tcgtgacggt gctggtagtc gtctacctgc
     5761 tggccaacct tctcgtcgat gtgctctacg ccgcgcttga t
//
LOCUS       DASWBF010000384        31211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_695255,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000384 DASWBF010000000
VERSION     DASWBF010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 31211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31211
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695255"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..489
                     /locus_tag="VF874_14230"
     CDS             25..489
                     /locus_tag="VF874_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177177.1"
                     /translation="MSQYGPPEDPNYYSEQPAAYSAYGQQPPGMPPGPPEEPPVPWYR
                     KPWALVLFGALGAIILVLILLLVFGGSNSDDNKPLSPVSTTSRTPGTTTATTTSPTTT
                     TTTTTTTETTETTETTTTSTTEPTETTTPTTTGGITITIPTSLPTIPGLPTP"
     gene            complement(518..1996)
                     /locus_tag="VF874_14235"
     CDS             complement(518..1996)
                     /locus_tag="VF874_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin-like GTPase family protein"
                     /protein_id="HEX9177178.1"
                     /translation="MGTSDQVRAILGGTIRAYRNDPTYRHRADLHRELDGIGWRLNQP
                     IRIALAGTLKAGKSTLVNALVGENIAPTDATEATRIVTWFRHGPTPKVTANHRGGRRT
                     NVPITRDDGLTFDLGNLDPNDVVDLDVEWPAAELIDATIIDTPGTSSLNSDVSARTLR
                     LLVPDDGVPRVDAVVFLLRTLNVADIALLKQIGELVGGSAGALGVIGVASRADEIGAG
                     RIDAMMSAKDVAKRFTEEMDKTGICQAVIPVSGLLALTARTLRQSEFVALQKLAGVDA
                     AELAKAMLSADRFVREDSSLPVDAATRTQLLDRFGMFGVRISIAVLCSGVSDSVGLAD
                     QLLERSGLVALRDVIDQQFAQRSDLLKAHTALLSLRRLVEVNPIPATGAIIADIDPLL
                     ADTHAFEELRLLSQLRSRPTTLTDDEMASLRRIIGGSGTDATSRLGLKADAPDDGPRA
                     AFAAAQRWRRRAEHPLNDPFTTRACRAAVRSAEALVAQYSYS"
     gene            complement(2010..3884)
                     /locus_tag="VF874_14240"
     CDS             complement(2010..3884)
                     /locus_tag="VF874_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin-like GTPase family protein"
                     /protein_id="HEX9177179.1"
                     /translation="MTQPSDPRQIRVIVELIEHTRKVAQTNHRDDLMDRLAAARDRIT
                     DPQIRVVIAGQLKQGKSQLLNSLLNMPVARVGDDESTVLTTIVLYGEQPAAQLVVATP
                     DGGAEAIAIPTADVSKDLRRAPQAGGRKVLRVEVIAPSPLLKGGLVFIDTPGVGGHGQ
                     PHLAATLGLLPDADAMLMVSDTSQEFTEPEMTFMRQAFEICPVAAIVATKTDLYPHWR
                     AVVDADRAHLQRVGVDMPLIPASSLLRHHAISLNDKELNDESNFPAIVAFLSDKVLSR
                     ENDRVRDHVLAELRSAADHLTLTLDSELSTLNDPDVRERLTADLERRKDEAQDALAAS
                     ALWQQVLNDGVADLTADVDHDLRSRFRAITQHFESIVDGCDPTLRWAEIGTELEQAVA
                     EAVGDNFVWAFQRAEALAKEVARTFEEVGLEPVKAPEISARQMGATFSGFKSLAQMDA
                     KPVSTGSKVVSGMRGSYGGVLMFGMLGSLAGLGMFNPLSVGAGLLLGRKAYKEDMENR
                     MLRLRNEAKNNVRRFVDDISFVVTKESRDRLQAIHRQLRNHYRGIANQTTRSLNESLQ
                     AMLTSARLEENDRNQRVAELDRQLNILKQVSAKAAELAASKPAATSTSPAQPRVTLRG
                     "
     gene            complement(3990..4991)
                     /locus_tag="VF874_14245"
     CDS             complement(3990..4991)
                     /locus_tag="VF874_14245"
                     /inference="COORDINATES: protein motif:HMM:NF038175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IniB N-terminal domain-containing protein"
                     /protein_id="HEX9177180.1"
                     /translation="MISLIDFILNLFRDRGAAADYVAHPEQALLDAGLGNVAPAQLAS
                     VAATAVPSLALGAGDPVIGLQRAVANNWGFEPVYAPSPTWSPSYAPQTDFLSGNQTDT
                     EFASHNAVPIMSPNQDAGANAQNGAFNLGFGDITLGDKTTASGQGAIAVSGNNSGDIL
                     TGDGSALGDNNDVYSGDIHSGPNSNVNYGNGNEVNEGNTAAGHDVVDTHDGTVVQTSG
                     QGTTTSSQGNTEWTAVRGNQTTTNIDGTGNDSGVDNTHTVVDTHTTTTTTSVADNSTH
                     DNTAAYNSANHDWGHLPLAGDSVHDNQSTYAPSYDASTHDNTTVQTHQSSHDLHLGF"
     gene            complement(5225..5725)
                     /locus_tag="VF874_14250"
     CDS             complement(5225..5725)
                     /locus_tag="VF874_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv0340 family IniB-related protein"
                     /protein_id="HEX9177181.1"
                     /translation="MANELLDFVMSLVRDPDAAARYAENPQQAIAGAHLTDVTSVDVN
                     NLIPVVSESLSMAAPTSGADAVGDANVWASGAATAAFDAFSEHLPTPVVVDTHDFIID
                     QPTVPAFDPGIEQFADPMPELPSYDAGTLDEPLVTPEVHDAGPVIDSGGIDPGFDHPA
                     PDHHIF"
     gene            5923..7803
                     /locus_tag="VF874_14255"
     CDS             5923..7803
                     /locus_tag="VF874_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX9177182.1"
                     /translation="MTDFLGLSIGMTNLVAARVGAPPVTRRAVLTVYPDRAPEVGVPS
                     ENPNLAEPGVVLSGFVERVGDPVPLVASDGSSHSADRLLVEALDAMTHTVADGTPPSQ
                     VAIAVPAHWGPAVLGALRAALRTEPNLSPNGVPPALVSDAVAALAALNTSPGLPDSGI
                     VALLDFGGSGTSITLADAANNFQPVDETLRFTEFSGAQIDQALLTHVLADIYQSSNAD
                     TASTAAVGALGRLREQCRQAKERLSAETATDLSVELPGYHSDIRVTRAELESVISGPL
                     AGVLGALDDMLQRNRIPAASLSAVATVGGGAGIPLITQRLSEHFRLPVVTTPQPALNT
                     AAGAALLAAAGPGADAPTGLAPAAADGPATGMATAAWAAGAAAAAATESAADGPSSAT
                     FRALAWSDEPGSDEPVPYTGADYTAEPAATSARPQMDFQPPEEPRPFAAEEPVAWYRR
                     PQVLFAAALAAVVLLSGGLWWTLTSASSPIRSIRTHTATAPATTNPETPGAPLTDTVT
                     FTGTDGSPTVVTPATTTTSEATSTTSATTTPSTTTTTTQTTTTTTTTTQPPTTTTTTT
                     QAPPTTTQAPPTTTQAPPTTTQAPPTTSAAFTTPQTITTVPVTTVPPIVTSPQPAAAS
                     AG"
     gene            7805..10279
                     /gene="iniR"
                     /locus_tag="VF874_14260"
     CDS             7805..10279
                     /gene="iniR"
                     /locus_tag="VF874_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoniazid response ATPase/transcriptional
                     regulator IniR"
                     /protein_id="HEX9177183.1"
                     /translation="MSAPAPRELPPQAREVVDGIAANPSASTKAVVVGGMGTGKTATL
                     AAIRAALREAGVEVMTRPVSPPRAGAAAVVDDAHLLADEELAALVDWVRDPSATVVIA
                     TQPYQHHGGLRALTVAMERENPPVLLGALPPQELHRVTAAALGGPPSAEVVRSLINAT
                     AGLPFLVHAALNHNTEAMAIAQAARFALIDRLRAVDEPVLDALLIASLSPDLGTADVA
                     AALRVSPDDAQMLVDRARGSGLVEPSHSPTFLRSVHRAVAQIVGAARHHDVEKALLAT
                     QLEMSTLSADLALRLAEHGVRNDRLAAVLETRAEQSAEQPDAAARLYRAALDAGATRL
                     TARLADALALTGDCATAGRLADDLLTSDDTADRAAAVRIAASVAVHDGNSGQAADLFR
                     WLGPHPDAFVSAAGAVVLLGAGDAAAARATLDAETAGPPTTAARAARSLADGLLHTLD
                     QPFAIAIGRLGQSLAGEQFARGITPDTAAALVTLAALHGGDPVRARSVISRAVRAGGE
                     THFAHRHRLLLGWVKMLDGGLASTGADVADESLHRRDALWAAALRTGIARRTGDSGAL
                     QRHWYTAMEVLAEYAIDLYSLLPLGELWVAAARLRQVARLQHALDEAFGVLERLDNPV
                     LWSVPLHWYGVHAGILANSPAAVAPHGQALTAAAGSSPFAKALAGAGRVWLRVLANHV
                     DLGEVTAAARGLAEYGQIWDATRLASQAALQTPDARVAGAMLQLARDLKATSVVDDQA
                     VETSRPPSAEPARSTPRESARLSEREREVAELLLLGMPYRDIGNQLFISAKTVEHHVA
                     RIRRRLGAESRSELLSMLRAMLAPQT"
     gene            10421..13207
                     /locus_tag="VF874_14265"
     CDS             10421..13207
                     /locus_tag="VF874_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX9177184.1"
                     /translation="MDTQTVIRLVVGLLMTAVVGVFALRRIWWLFTLVRSGQPATGRT
                     DDLGTRIRTQVEEVFGQTRLLRWSIPGLAHFFTMWGFFILLTVYIEAYGVLFSPKFAI
                     PFIGHWPALGFVQDFFALAVLLGITTFAIIRLRTGPKEHGRESRFYGSHTGGAWLILF
                     MIFNVIWTYALFRGSSVNNGNLPYNWAAFFSHGMGKALEWLGPTGNEYLETVSLLLHI
                     GVMLAFLIIVLHSKHLHIFLAPINVTFKRLPDATGPLLPMEYDGKPIDFEDPDENAVF
                     GRGKIEDFTWKGMLDFATCTECGRCQSQCPAWNTGKPLSPKLVIMDLRDHWMAKAPYL
                     LGKDPGPLEGLDLETEHEEAHHVPESGFGRVPGSGPEQVARPLVGTAEQGGVIDPDVL
                     WSCTTCGACVEQCPVDIEHIDHIVDMRRYQVMMESEFPSELSVLFKNLETKSNPWGQN
                     AKDRLNWINEVEFDVPVYGVDAESFDGYEYLFWVGCAGAFEDRAKKTTKAVAELLSIA
                     GVKYLVLGAEETCNGDSARRSGNEFLFQQLAQVAVETLNGVFEGVETVDRKIVVTCPH
                     CFNTIGREYKQLGANFTVLHHTQLLNRLVREKKLVPVSPMNSDVTYHDPCYLGRHNKV
                     YEAPRELIGASGVTLKEMPRHAETGLCCGAGGARMWMEEHIGKRVNHERVDEALMTVS
                     NGAKIATGCPFCRVMMTDGLTDRQESGKGEGVEVLDVAQLLLGALDTSTVTLPPKKKP
                     EEKAAPAPAAAAAPSAPPAEKTAPAAQQEAKPVTGLGMAGGAKRPGAKKSDATAESAQ
                     AAPAKGLGLAAGAKRPGAKKAAPAAEASVTAAEPAPTATEPASTAAEPASTAAEKPVK
                     GLGIAAGARRPGAKKSTPAAAPAPAEPKPEAAQPEPTPTPPPADDGDGAGSASAPPVK
                     GLGIARGARPPGKR"
     gene            13266..13763
                     /locus_tag="VF874_14270"
     CDS             13266..13763
                     /locus_tag="VF874_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177185.1"
                     /translation="MEVGSEATVDTQGIGQAMIEHYAELRRQRLFHAHGGDYVLRRDS
                     AAGRFHVYLRARGSSPDVVEFRARSFTRFPHAERTRLLEWVNRFNDRIQWLPASVRNS
                     RYSADLAVVGNSRFCVAGAGDFAAFVRFADLFLAVAAKLFEGVDAEMKLPTPAELELW
                     FQRSG"
     gene            13863..14309
                     /locus_tag="VF874_14275"
     CDS             13863..14309
                     /locus_tag="VF874_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX9177186.1"
                     /translation="MIERYAKKRSERLFRANDGEYVLLQDSARGHFPVYMHARGSSPD
                     VVTLRAHWSARCAHTKRIHLRECVNRFNDRNPWLTVSVREAHDSSALTVVVNSSILLA
                     DEVDFGVFVRFVDLSLASAAKLFEQVYPGTQLPSPAELRRCLQLPA"
     gene            14365..15630
                     /locus_tag="VF874_14280"
     CDS             14365..15630
                     /locus_tag="VF874_14280"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9177187.1"
                     /translation="MTTHQLPWHATGHHHPQRTFAQSTKLQDVLYEIRGPVHDHAARL
                     EAEGHRILKLNIGNPAPFGFEAPDVIMRDMIQALPYAQGYSDSKGILPARRAVVTRYE
                     LVDGFPRFDVDDVYLGNGVSELITMTLQALLDNGDQVLIPAPDYPLWTASTSLAGGTP
                     VHYLCDETQGWQPDVADIESKITERTKALVVINPNNPTGAVYSREVLEQMADLARKHQ
                     LLLLADEIYDKILYDDAKHVCVASVAPDLLCLTFNGLSKAYRVAGYRAGWLAITGPKE
                     HASSFLEGISLLANMRLCPNVPAQHAIQVALGGHQSIEDLVLPGGRLLEQRDVAWNKL
                     NEIPGVSCVKPQGALYTFPRLDPEVYDIVDDEQLVLDLLLQEKILVTQGTGFNWPAPD
                     HLRIVTLPWARDLAAAIERLGNFLASYRQ"
     gene            complement(15634..16500)
                     /gene="rfbA"
                     /locus_tag="VF874_14285"
     CDS             complement(15634..16500)
                     /gene="rfbA"
                     /locus_tag="VF874_14285"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX9177188.1"
                     /translation="MRGIILAGGSGTRLYPITMGVSKQLLPVYDKPLIYYPLSTLIMA
                     GIRDILVITTPADTASFERLLGDGSAFGIDISYAVQEKPDGLAQAFVIGADHIGTDSV
                     ALVLGDNIFYGPGLGSNLSRIQNVSGGAIFAYWVANPSAYGVVEFGADGTPLSLEEKP
                     ATPKSHYAVPGLYFYDNEVVEIARSLRPSARGEYEITEINQTYLNRGQLSVEVLTRGT
                     AWLDTGTFDSLLDASDYVRTIERRQGLKISVPEEVAWRVGLIDDEQLAARANELLKSG
                     YGGYLLELLDEK"
     gene            complement(16509..16910)
                     /locus_tag="VF874_14290"
     CDS             complement(16509..16910)
                     /locus_tag="VF874_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9177189.1"
                     /translation="MTVSEIATVLAWHDALNTQDVDTLVELSTGDIEIGDAHGAVQGH
                     GALREWATSKGFLIEPGRMYVHDGVVVVEQHIGDAGSPQVGDERLREERTVMASAFRV
                     VHDKVTSVFRHDDLETALAATELSEDDIFVG"
     gene            complement(16928..17662)
                     /locus_tag="VF874_14295"
     CDS             complement(16928..17662)
                     /locus_tag="VF874_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX9177190.1"
                     /translation="MDFAAALIDENAAFAEVLRGADPATPVPTCPGWTLRQLTRHVGR
                     GDRWAAQIVGDRLTDFLDPRDVRGGKPPDDGDGTIQWLHDGARSLVEAVDKTGADTGV
                     WTFLGPRPAGWWIRRRLHEVTVHRADAELALGRDFRLSADLAADGLTEWLEWVVIQAG
                     GERMPLADGQSLHLHASDGGEWTLRSDGENLQLARHHEKSTVAVRGDAASLLLAMTRR
                     IPADDPRLTVFGDGDVWRGWLDRTPF"
     gene            complement(17664..18950)
                     /locus_tag="VF874_14300"
     CDS             complement(17664..18950)
                     /locus_tag="VF874_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9177191.1"
                     /translation="MRQRIRWLALHGVVRGLASIGMRRGEPQARMIFDPAVRANPMPF
                     IDELRAMGRIVRGRVVYMTVDYEIAHELLRSEDFRVTALGGNLPKPLRWVTEKTDTGL
                     LHPLAPPSLLSVEPPEHTRYRKLVSSVFTTRAVAALRDRVEETAKVLLDDLGDGSGAV
                     DIVARYCSQLPVTIIGDILGVPDEDRAQILHFGELGAPSLDIGLSWRQYQQVNQGIVG
                     FNAWLAEHLRQLRRNPGDDLLSQLTQAADEGGRLNDRELQATAGLVLAAGFETTVNLL
                     GNGIRMLLEHPQHLVTLSKRPELWPNAVEEILRFDSPVQLSARVARCDIDVAGTTIRR
                     GEMVVLYLAGANRDPAVFEDPHRFDIERPNANKHLSFSSGRHFCLGAALARAEGEVGL
                     RSFFDRYPDARLAGEGTRRDTRVLRGWSSLPVTLEA"
     gene            complement(18947..19273)
                     /locus_tag="VF874_14305"
     CDS             complement(18947..19273)
                     /locus_tag="VF874_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX9177192.1"
                     /translation="MTVYALNLFNVADRDEYLAYSRRSVAEVTAHGGKVVALGKFRRA
                     ITGDVAPRQVLILVEWESEAAFDGYCNDPALADLHPHRENGSSDYVWQLFDRLDDLRP
                     VLKLDS"
     gene            complement(19280..20614)
                     /locus_tag="VF874_14310"
     CDS             complement(19280..20614)
                     /locus_tag="VF874_14310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX9177193.1"
                     /translation="MRCTVFGTGYLGATHAAGMAELGHEVVGVDIDPGKVAKLSAGEA
                     PFYEPGLRKMLRDNISAGRLRFTTDYDVAADFADVHFLGVGTPQKKGEYGADLRHVHA
                     VIDALVPRLRRPAVIVGKSTVPVGTAAELATRAAALQRDGVAVEVAWNPEFLREGFAV
                     HDTLHPDRIVLGVQANSQRAERAVRDLYAQLLDAGVPFLCTDLQTAELVKVSANAFLA
                     TKISFINAISEVCEAADADVSVLADALGYDARIGRRFLNAGLGFGGGCLPKDIRAFMA
                     RAGELGASQALTFLREVDSINMRRRTKMVELAARACGGSLLGANVAVLGAAFKPESDD
                     VRDSPALNVAGMLQLNGAAVNVYDPKANENSQRVFPTLNYSTSVTEACDRADAVLVLT
                     EWREFVDLDPDELVGTVRARVVVDGRNCLDVARWTTAGWQVYALGRRPRRVA"
     gene            complement(20689..22224)
                     /locus_tag="VF874_14315"
     CDS             complement(20689..22224)
                     /locus_tag="VF874_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177194.1"
                     /translation="MDIVLGVSMTPTTVRMVLVEGEKADGRTVDHDIFDITAVDGAAN
                     SSASDQVISAILGTQQSAAAGGHRLITTGVTWTNHDDASALRDALTARGLDEVMLVSE
                     LHAAGAIAQAAGRAVGYDKTALMFVERNTATLSVVEAHDGSIVKVVSRSLHSADAMAV
                     MAEMVTSLEAQDVRPGGVFVVGSGVDVASVKEHLENLVSLPVSAPEDSEMALARGAAL
                     AAVSAPRFEATTIGLAYSQDPDPAAFAATQMRPTSAASPVDDDDDVVADESRRPFMLV
                     GSTLTSIFVVGVVALVISLAVSIRPTVDQRPNPGESIVVPSTSAPAPPAAQQVEPPPA
                     EPPKAPETIPAPVPVVQEQPQQAPRQVFVEPPQAPAEVPAAAPPPAPAAPPPAPVPAA
                     PTPVIAPPVIIPPIIQLFPQPSYQSPQYPWYPQQPQWTPPWQPPTTQPQQPPSTPPYR
                     PPVYDEPDEPQVTLIPRQPVPQQPVVPQQPVITQQPRQPVIQLPQLPSLGGNRTPFWE
                     YWD"
     gene            complement(22439..23020)
                     /gene="dcd"
                     /locus_tag="VF874_14320"
     CDS             complement(22439..23020)
                     /gene="dcd"
                     /locus_tag="VF874_14320"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX9177195.1"
                     /translation="MLLSDRDIRAEIAAGRLGLEPFDDKLVQPSSVDVRLDSLFRVFN
                     NTRYTHIDPALQQDELTSLVEPLSGEPFVLHPGEFVLGSTLELCTLPDDLAGRLEGKS
                     SLGRLGLLTHSTAGFIDPGFSGHITLELSNVANLPITLWPGMKIGQLCLLRLTSPAEH
                     PYGSAKVGSKYQGQRGPTPSRSYQNFIGPSPRT"
     gene            23081..23151
                     /locus_tag="VF874_14325"
     tRNA            23081..23151
                     /locus_tag="VF874_14325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23113..23115,aa:Gly,seq:ccc)
     gene            23466..24479
                     /locus_tag="VF874_14330"
     CDS             23466..24479
                     /locus_tag="VF874_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX9177196.1"
                     /translation="MNTIDLVIVALGCGVFARALARYAPIAVPGAGLAAQGVLGVYTG
                     LMVRSISFAALGSNWPIVVAVAVGTLVISVAGGALLGLHRDVSPLTGGLALVAGGSSG
                     LVAIARELGGDDRVVAAVQYLRVALITAAMPVVVTVFYHATGAHGSQISETTSLPWYF
                     GLPLIAVIVVVGAAAGRSVRLPGSGLLGPMAITIVFELSGLTAGLTVPMVLVQAGIML
                     IVWQTVLAFNRESLRAIQRILPGAFALIMVLNVAAAGLGVVLANVAGLSMLDGYLATS
                     PGGIYAVLGTAVGSGSNVPFVMAAQVIRILLMLFAAPFVARLFMRFTPQSAASRELIP
                     VAA"
     gene            24627..24806
                     /locus_tag="VF874_14335"
                     /pseudo
     CDS             24627..24806
                     /locus_tag="VF874_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(24813..25583)
                     /locus_tag="VF874_14340"
     CDS             complement(24813..25583)
                     /locus_tag="VF874_14340"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX9177197.1"
                     /translation="MAEGRILTNSASEYWHGDGALLHVDPYTGKDLPEDPDVRTYLIA
                     GTDHFGSSRIKEALPAANPVHRLDVTPVNRALLVALENWVTDGVEPPPSQVPRTSKGT
                     AVSRKDVLSGFGHTAAPDPASLSYARSVNLGPDVDRGIGHWPVKLGETYVDLVSAIDA
                     DGNEIAGIRLPAVAAPLATYTGWNPRRHMDELPDVLYERIGSKLPFPPGRPSATERYP
                     TRDDYSAAVRAAAESLVAERFLLTEEVDAVVKKAVADY"
     gene            complement(26020..29274)
                     /locus_tag="VF874_14345"
     CDS             complement(26020..29274)
                     /locus_tag="VF874_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9177198.1"
                     /translation="MAVYTVRRPKAVLAALLVLLGISIVFGGSVSDKLGVGGYTDPTS
                     ESSQADEFLDQNFGTTSNLVIQLLPREGTVDSIDVATVRDRVRQLVEAEPDAKVTRSF
                     GDEGATDLRSKDGRSGLMLVHVGGTADEAAATASRIIARLPDDPNVTVRAGGSLGVQQ
                     EIRAKVKHDLKLSESIALPISLAVLVIVFGGLVAAFLPVIVGISSIVTTLSVLLLMTK
                     ITDVSVHALTVATAFGLGLSIDFGLLMVSRFREERDNGKDHHQAIIATVTTAGRTILF
                     SAATVTLAMTGLLVFPTYFLRSVGITATATVMLSAFSAIVVLPALLALLGKRIDSLAV
                     IRRKVPLSADSLFWRRFAQAVTRRPLFYALPVVAVLLGLGIPFLSAQYATPDERALPT
                     DSNARLVSESLQKDFQLDPSQAITLVTRTDTGVLKDLAAEVSRMDGVVLVSGPIGRYE
                     HGQIVGQTPATAESGASYAFVYLSVQADSDVAQHLVHDIRAKIADHQVEVGGPTATLI
                     DSRAAIADRLPLAIALIAVSTFILLFLFTGSVVVPTKALLLNLLVLSAVLGVMVWIFQ
                     EGHLASVLGITPAPLNLSMVVLLCSIAFSLSVDYEIFLLSRIKEARDSGLSNNAATVV
                     GLGRVGRIVTSAALLLTITLISFANGLAFMKMFGIGTALAVVIDATIIRGVVVPAFLR
                     VAGDLNWWAPRPLRWLHSRIGISEAPSDDETAPPVPHTPEEMPHQIAKQVADEKTVVC
                     HMPVRPPSEVEVIPGRHLVANVNGTVIVVAQRDRAPLSQQSVAAQQLAALAEMVRHTD
                     ARRLTSAFSQLTRQTTWSRIFVDVGIIMPTPAGLDVLLCGSVTVVLDDGVGRTVLHGR
                     GRLLHRSVPVPGVAAIVTVDEVSQRPLAPAQRNGVYKLTGGTVPAQGAVVWSTQASPA
                     PRRAPAMPSPARGDRIPTPAAVEFVRPKRWVVLDDNTRFDIERDCVIGRDPYGSDAAK
                     RGLRPVRIDDRTGEMSRAHVEVRIVNGEVIIVDRNSTNGVFVREPAQQGWTRLAPWQS
                     AKWRPGAYVQIGGRILRLHVPTAHGPQRGPRVNVQHDIPQQLHAGRAYAAAIAK"
     gene            complement(29418..30401)
                     /locus_tag="VF874_14350"
     CDS             complement(29418..30401)
                     /locus_tag="VF874_14350"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX9177199.1"
                     /translation="MSNAATQEKSGTRRRPMAGPGVWNHASLRAEHYIITVEPQQAAA
                     CLRIRDELRRRGTRLESIEPSDFQDPALAEIGRGIRHRIRSGPGFCLLRGLPFQGWTD
                     EEASMLYWGLGTYLGGPQPQNRQGDRVYLVQDTGKSVAEARGSKTNSELIFHTDSASG
                     YVGIQADVLGLLCLRKAVSGGESLIVSGHTAYNALLETQPELVEELYGEFCFDRSKET
                     GPGEDPVSVGAVFTDTADGVRVRYNRAYIELGHMLAGRPLGERQREALDAFDRFLNDP
                     AHSVEFKLDPGDVLFADDYVTLHNRRRFVDAPEVGNRRCLVRLWLAGEPRN"
BASE COUNT         5108 a        10373 c        10451 g         5279 t
ORIGIN      
        1 cgacacgctt taacatcaag ccccgtgtct cagtacggtc cacccgaaga cccgaactac
       61 tactccgaac agccggccgc ttacagcgcc tacggccagc aaccgcccgg catgccccca
      121 ggtccgccgg aggaaccgcc ggtcccgtgg taccgcaaac cgtgggcgct ggtcctgttc
      181 ggcgccctcg gcgcgatcat tctggtgctg atcctgctgc tggtcttcgg cgggtcgaac
      241 tccgacgaca acaagccgtt gagtccggtg tcgaccacgt cacgcacccc gggaacgacg
      301 accgcgacca cgacgtcgcc gacgacgacc accactacca cgacgaccac tgaaaccacc
      361 gaaaccaccg agacgaccac cacgagcacc acggaaccga cggaaaccac gacgccaacg
      421 acgaccggcg gcatcacgat cacgatcccg accagccttc cgacgattcc tggcctgccg
      481 acgccgtgag tcgtcgggcg gcgaggctcg ctcagaacta gctgtacgag tactgcgcca
      541 ccagcgcttc ggcgctgcgc accgcggccc ggcacgcccg cgtcgtgaac gggtcgttga
      601 gcggatgctc ggcgcgccgg cgccagcgct gcgccgcggc gaacgcagca cggggtccgt
      661 cgtcgggggc gtcggccttc aggccgagcc ggctggtcgc gtcggtgccc gagccgccga
      721 tgatccggcg cagcgatgcc atctcgtcgt cggtcaatgt cgtgggccgc gaacgtaatt
      781 ggctcagcag ccggagctct tcgaatgcgt gcgtgtcggc gagaagcggg tcgatgtcgg
      841 cgatgatcgc gcccgtcgcc ggaatcggat tgacttcgac aagacgccgc agcgacagaa
      901 gcgcagtgtg cgccttcagc aggtccgagc gttgcgcgaa ctgctgatcg atgacgtcgc
      961 gcagcgccac cagcccgctg cgctccagca gctggtcggc cagccccacc gagtcgctga
     1021 cgccagagca cagcaccgcg atcgagatcc ggacaccgaa catgccgaat cgatccagca
     1081 gctgcgtgcg ggtggcggcg tcgacaggaa gcgaactatc ttcgcgcaca aagcgatccg
     1141 ctgacagcat cgccttggcc agttccgcgg cgtccacccc ggcgagcttc tgcagcgcga
     1201 cgaactcgct ttgccgcagc gtgcgtgcag tcagtgcgag caggcccgac accggaatga
     1261 cagcctggca gatgccggtc ttgtccatct cttcggtgaa ccgcttcgcg acgtctttgg
     1321 ccgacatcat cgcgtcgatg cggcccgcgc cgatctcgtc ggcacgcgac gcgactccga
     1381 tcactcccag cgcacccgcc gacccgccga ccagttcgcc gatctgcttg agcaatgcga
     1441 tgtcggccac gttgagcgtg cgcagcagga agacgacggc gtcgacgcgc ggcacgccgt
     1501 cgtcgggcac gagcagccgc agtgtgcgcg cggagacgtc gctgttcaac gacgacgtgc
     1561 ctggcgtgtc gatgatcgtc gcgtcgatga gctcggctgc cggccattcc acgtcgaggt
     1621 ccacgacgtc gttggggtcg aggttgccga gatcgaacgt caagccgtca tcacgggtga
     1681 tgggcacgtt ggtgcgtcgg ccgccccggt ggttggccgt gaccttcggg gtggggccgt
     1741 gccgaaacca ggtgacgatg cgggtggcct cggtggcgtc ggtcggcgcg atgttttccc
     1801 cgacgagcgc gttcacgagg gtggatttgc ccgctttgag cgtgccggcc aaggcgatgc
     1861 ggatcggctg gttcagccgc cagccgatcc cgtccagctc ccggtgcagg tccgcccggt
     1921 gccggtaggt ggggtcgttg cggtaagcac ggatcgtgcc gccgaggatg gcgcggacct
     1981 gatcgctggt gcccaccggt agattttagt tatccgcgta gcgtgacccg tggctgcgcc
     2041 ggggatgtgc tggttgcggc cggtttcgac gcggccagct cggccgcttt cgccgacacc
     2101 tgcttgagga tgttgagctg acggtccagt tcggcgactc gctggttgcg gtcgttctct
     2161 tccagccgcg ccgacgtgag catcgcctgc agcgactcgt tgagcgaccg ggtggtctgg
     2221 ttcgcgatgc cgcggtagtg attgcgcagc tggcgatgga tggcctgcag ccgatcgcgg
     2281 gactccttgg tgacgacgaa cgagatgtcg tcgacgaacc ggcgcacgtt gttcttcgcc
     2341 tcgttgcgca accgcagcat ccggttctcc atgtcctcct tgtacgcctt gcggcccaac
     2401 agaagtccgg caccgaccga cagcggattg aacatgccca acccggccag cgagccgagc
     2461 atgccgaaca tcagcacgcc gccgtaggag ccgcgcatcc cgctgaccac tttgctgccg
     2521 gtgctgacgg gcttggcgtc catctgggcc agcgacttga acccgctgaa cgtggcgccc
     2581 atctggcgcg cgctgatctc cggcgccttc accggttcca ggccgacctc ttcgaacgtg
     2641 cgggccactt ccttcgccag cgcctcggcc cgctggaagg cccagacgaa gttgtcgccg
     2701 accgcctcgg ccaccgcctg ttccagctct gttccgatct cggcccagcg cagcgtcgga
     2761 tcacagccgt cgacgatgct ctcgaagtgc tgggtgatcg cgcggaaccg cgaccgcagg
     2821 tcgtggtcga cgtcggcagt cagatcggcg accccgtcgt tgagcacctg ctgccacagc
     2881 gccgaggcgg ccagtgcgtc ctgcgcctcg tccttgcgcc gttccaggtc ggcagtgagc
     2941 cgctcacgca cgtcgggatc attcaacgtc gacagttccg agtcgagcgt cagcgtcaaa
     3001 tggtctgcgg ccgaacgtaa ctcagcaagc acatggtcgc ggacccggtc gttctcccgg
     3061 gacagcacct tgtcggacag aaacgccacg atcgccggga agttcgactc atcgttgagt
     3121 tccttgtcgt tgagcgagat cgcgtggtgg cgcaacagcg acgacgccgg gatcaacggc
     3181 atatcaacgc cgacccgctg tagatgggcg cggtcggcgt cgacgaccgc gcgccagtgc
     3241 gggtagaggt ccgtcttcgt cgcgacgatc gcggccaccg ggcagatctc gaacgcctgg
     3301 cgcatgaacg tcatctcggg ttcggtgaac tcctggctgg tgtcgctgac catcagcatc
     3361 gcgtcggcgt cggggagcag gccgagcgtg gccgcgaggt gtggctggcc gtggccgccc
     3421 acgccggggg tgtcgatgaa caccagcccg cctttgagca gcgggctggg cgcgattacc
     3481 tcgactcgaa gaaccttgcg gccgccggcc tgcggggcgc ggcgcaggtc cttgctgacg
     3541 tcggcggtcg gaatcgcaat cgcctcggcc ccgccgtcgg gggtggcgac gacgagttgt
     3601 gccgccggct gctcgccgta gagaacgatc gtggtcagca ccgtcgactc gtcgtcgccg
     3661 acccgggcca ccggcatgtt caacagcgag ttgagcagct gactcttgcc ctgcttcagc
     3721 tggcctgcga tcaccacccg gatctggggg tcggtgatcc ggtcccgggc cgcagcgagc
     3781 cggtccatca agtcgtcccg gtggttcgtc tgggcgacct tgcgggtgtg ctcgatcagc
     3841 tcgacgatga cccggatctg cctcggatca ctgggctggg tcacgtgtcg ttctcccact
     3901 cactcgtacg tccacggtat gcgcacaaac ctggcgggga ccattgcggt ccccgccagg
     3961 cggtccacca actggcttgc ggtcgacgat cagaacccga gatgcaggtc gtggctgctc
     4021 tggtgggtct gcaccgtggt gttgtcgtgc gtcgaggcgt catacgacgg cgcgtacgtc
     4081 gactggttgt cgtgcacgga gtcccccgca agcgggaggt gcccccagtc gtggttcgcg
     4141 ctgttgtacg ccgcggtgtt gtcgtgcgtc gagttgtcgg ccaccgacgt cgtagtcgtg
     4201 gtggtgtggg tgtccacgac ggtgtgggtg ttgtcgacgc cgctgtcgtt gccggtgccg
     4261 tcgatgttcg tcgtggtctg gttcccgcgc accgcggtcc actcggtgtt gccctgagac
     4321 gacgtggtgg tcccttgccc ggaggtctgg accaccgtgc cgtcgtgcgt gtcgaccacg
     4381 tcgtggccgg cggcggtgtt gccctcgttc acctcgttgc cgttcccgta gttgacgttc
     4441 gagttgggac cgctgtggat gtcgccgctg tacacgtcgt tgttgtcgcc caacgccgat
     4501 ccgtcgccgg tgaggatgtc gccgctgttg ttgccactga ctgcgatggc gccctgtccg
     4561 ctggccgtgg tcttgtcgcc gagggtgatg tcgccgaagc cgaggttgaa cgcgccattc
     4621 tgggcgttgg cacccgcgtc ctgattgggg ctcatgatcg ggacggcgtt gtggctcgcg
     4681 aactcggtgt cggtctggtt gccgctgagg aagtcggtct gcggcgcgta cgacggcgac
     4741 caggtgggcg acggcgcgta caccggctcg aagccccagt tgttggccac cgcacgctgc
     4801 aggccgatca ccgggtcacc ggcgccgagc gcgaggctgg gcaccgcggt ggcggccacc
     4861 gacgcgagct gcgccggcgc gacgttgccc aggccggcgt cgaggagcgc ctgctcgggg
     4921 tgcgcgacgt agtcggccgc cgcgccgcgg tcgcggaaca ggttcaggat gaagtcgatc
     4981 aagctgatca tcgaagtacc ctttcggttg tcttcaccgg gctgtccggt gatctgctag
     5041 ccacggtatg gaccggcaat ttgtccggaa acggggtcga gtcccctagc tcgacgaccc
     5101 catagtgggg taactcagca acatcggttc ggggattagg ggattgttag ggttgatgcg
     5161 ggaaacgcga cagcccccgc acgccgggcg tgcgagggct gtcgcgtacc gcgttagaag
     5221 atcgctaaaa gatgtggtgg tcgggcgccg ggtgatcgaa gccggggtcg atgccgcccg
     5281 agtcgatcac cggccccgcg tcgtgaacct cgggtgtgac aagtggttca tcgagcgtgc
     5341 cggcgtcata actaggcagc tcaggcatcg gatcggcgaa ctgctcgatg cccgggtcaa
     5401 aagcagggac cgtcggctgg tcgatgatga aatcgtgggt gtcgacgacc acaggggtgg
     5461 gcaggtgctc ggagaacgcg tcgaatgccg ccgtcgccgc gccgctggcc cacacattcg
     5521 cgtcgccgac agcgtccgcg ccggaggtcg gggcggccat ggacagcgac tcggacacca
     5581 ccggaatcag gttgttgacg tcgacgctgg tcacatcggt cagatgggcg ccggcgatcg
     5641 cctgctgcgg gttctcggcg tagcgtgcag cggcgtcggg gtcgcgcacc agcgacatca
     5701 cgaagtcgag cagctcatta gccatgcccc gatgctatcc gtcaccagct aataacggat
     5761 cggtgtcgaa gccactgtct acagcctggc gttaggggat atgccgttag gggctgcgac
     5821 accgttaggg ggtgtcgcat gccggcggta ctttctgccg ctatcgtgag gaacggccgc
     5881 cgacgctgcc atttaactgg gtgaaggaca ggactcttcc agatgaccga ctttctcggg
     5941 ttgtccatcg ggatgaccaa cctggtcgca gcgcgcgtgg gcgctccgcc ggtgacgcgc
     6001 cgcgccgtgc tgacggtcta ccccgacagg gcccccgagg tgggggtgcc gtcggagaac
     6061 cccaacctgg ccgagcctgg tgtggtgctg tccggcttcg tcgagcgcgt cggcgacccg
     6121 gttccgctcg tcgcatccga cgggtcaagc catagtgcgg acaggcttct cgtcgaggcg
     6181 ttggacgcga tgacgcacac ggtcgccgac gggacacccc cgtcgcaggt cgcgatcgcc
     6241 gtacccgcgc actggggtcc cgctgtcctc ggcgcgttgc gagcagcgtt gcgcaccgag
     6301 ccgaacctgt cgcccaacgg cgttcctccg gcgttggtgt ccgatgccgt cgccgccttg
     6361 gccgcattga acaccagccc cggtttgccc gatagcggga tcgtcgcgct gctggacttc
     6421 ggtggcagcg gcaccagcat cacgctcgcc gacgcggcca acaactttca gccggtcgac
     6481 gaaaccctgc gattcaccga gttttccggc gcgcagatcg accaggcgct gctcacccac
     6541 gtgctcgcgg acatctacca gtccagcaac gccgacaccg ccagcaccgc ggcggtcggc
     6601 gcgctcggcc gcctgcgtga gcagtgccgc caagccaagg agcggctgtc cgccgagacc
     6661 gccaccgacc tgtcggtcga gcttcccggc taccactccg acatccgggt gacccgtgcc
     6721 gagctggaga gcgtgatcag cggaccgctc gccggcgtcc tcggtgcgct cgatgatatg
     6781 ttgcagcgca accgaattcc cgccgcatcg ctgtcggcgg tggctacggt cggcgggggt
     6841 gccgggattc cgctgatcac ccagcggctg tccgaacact tccgcctacc ggtggtgacc
     6901 acgccgcagc cggcgctgaa cacggcggcc ggcgcagcac tgctcgcggc cgcagggccg
     6961 ggcgccgatg cgccgaccgg gctggccccg gcggccgccg atggacctgc caccgggatg
     7021 gcgaccgcgg cgtgggccgc cggagctgcg gccgcggccg cgacggaatc ggccgctgat
     7081 ggcccttcgt ccgcgacgtt ccgcgcactc gcgtggtcgg acgagccggg cagcgacgaa
     7141 cctgtgccgt atacgggtgc cgattacacc gccgagccgg cggccaccag cgcgcggccg
     7201 cagatggact tccagccgcc cgaggagccg cgaccgttcg cggccgagga gccggtggcg
     7261 tggtaccgcc gaccccaggt gttgttcgcg gcggccttgg ccgcggtggt gctcttgtcg
     7321 ggcgggctgt ggtggacgct gaccagcgca tccagcccga tccggtccat ccgtactcac
     7381 accgccacgg ctccggccac caccaacccc gaaaccccgg gcgcgccgct gaccgacacg
     7441 gtgacgttca ccggcaccga cggtagtccg accgtcgtga caccggcgac gacgacgacg
     7501 agcgaggcga cgtcgaccac cagcgccacc accaccccgt cgaccacgac aaccaccacc
     7561 cagaccacga cgaccacgac gaccaccacc cagccgccca caaccaccac caccaccacc
     7621 caggcgccgc cgaccaccac ccaggcgccg ccgaccacca cccaagcacc accgaccacc
     7681 acgcaggcac caccgaccac gagcgcggcg ttcacgactc cgcagaccat caccacggtg
     7741 cccgtcacga cggtgccgcc gatcgtcacc agcccacagc cggccgccgc gtcggcgggg
     7801 tgacatgagc gctccggcgc ctcgcgaact tccgccgcag gcacgtgagg tcgtcgacgg
     7861 gatcgcggcg aatccgagcg cgagcaccaa ggccgtcgtc gtcggcggca tgggcaccgg
     7921 caagacggcc acgctggccg cgatccgcgc tgcgctgcgt gaagccggcg tcgaggtgat
     7981 gacgcggccg gtgtcgccgc cgcgggcggg tgccgccgcg gtcgtcgacg acgcgcatct
     8041 gctcgccgac gaagagttgg ccgcgctggt cgactgggtg cgcgatccgt cggcaacggt
     8101 ggtcattgcg acgcagccgt atcaacatca cggcgggctg cgcgcgctca ccgttgcgat
     8161 ggagcgcgag aatccgcccg tgctgctcgg tgcgcttccg ccgcaagaat tgcaccgcgt
     8221 caccgctgca gcgctcggcg gcccaccgtc ggcagaggtg gtccgcagtc tgatcaacgc
     8281 gacagcgggc ttgccgttcc tggtgcacgc ggcgctgaac cacaacaccg aggcgatggc
     8341 gatcgcgcag gccgctcggt tcgcgctgat cgaccggttg cgcgcagtcg atgaaccggt
     8401 gctcgacgcg ctgctgatcg cctcgctgag ccccgacctg gggacggcag acgtcgccgc
     8461 ggcgctgcgc gtatcccccg atgacgccca gatgctggtc gaccgggcac gaggcagcgg
     8521 ccttgtggag ccgtcgcaca gcccgacttt cctgcggtcg gtgcaccgcg ccgtcgcgca
     8581 gatcgtcggc gccgcacgtc accatgacgt cgaaaaagcg ctgttggcaa cgcaattgga
     8641 gatgtcgaca ctgtcggcgg atctcgcgct gcgcctcgcc gaacacggcg tgcgcaacga
     8701 ccggctcgcc gccgtgctcg aaacgcgcgc cgagcagtcg gcggagcagc cggatgccgc
     8761 ggcgcggttg taccgcgcgg ccctcgacgc cggtgcgaca cggctgaccg ccaggcttgc
     8821 ggacgcgctg gcgctcaccg gcgactgcgc aacggcgggc cggctggccg acgatctgct
     8881 gacgtccgac gacaccgccg accgcgccgc ggcggtacgg atcgccgcgt cggtggccgt
     8941 ccacgacggt aactccggcc aggccgccga cctgttccgt tggctggggc cccacccgga
     9001 cgcgttcgtc agcgccgccg gcgccgtggt gctgctcggc gcgggcgacg ccgccgccgc
     9061 gcgggcgacg ctggacgccg agaccgccgg gccaccgacc accgccgccc gggccgcccg
     9121 cagcctggcc gacggcctgc tgcacacgct ggatcagccg ttcgcgatcg cgatcggccg
     9181 gctcgggcag tcgctggccg gtgaacaatt cgcgcgcgga atcactcccg acaccgcggc
     9241 cgcgctcgtc acactcgccg ctttgcacgg cggcgacccg gtgcgcgccc gcagcgtgat
     9301 cagccgcgcc gtccgcgccg gaggcgagac ccacttcgca caccggcacc ggctgctgct
     9361 cgggtgggtg aagatgctcg acgggggact ggcctccacg ggcgcagacg tcgccgacga
     9421 gtcactgcac cgacgcgacg cgctctgggc ggccgcgctg cgtaccggca tcgcgcggcg
     9481 caccggcgac agcggcgcgc tgcagcgcca ctggtacacc gcgatggagg tgctggcgga
     9541 atacgcaatc gacctgtact cgctgctgcc gctgggcgag ttgtgggtcg cggctgcgcg
     9601 gctacgccag gtcgcgcggc tgcaacacgc gcttgacgag gcgttcgggg tgctggagcg
     9661 actcgacaac ccggtgctct ggtcggtgcc gctgcactgg tacggcgtgc acgccgggat
     9721 tctggcgaac tctcccgcgg cggtcgcgcc ccacggccag gcgctcaccg cggctgcggg
     9781 ttcgagcccg ttcgccaagg cgctggccgg cgccggacgg gtgtggctgc gggtgctggc
     9841 caaccatgtc gacctgggtg aggtcaccgc ggcggcgcgc ggcctggccg agtacggcca
     9901 gatctgggat gccacgcggc tggccagcca ggcggcgctg cagacgccgg acgctcgggt
     9961 ggccggcgcg atgctgcagc tcgcacggga cctgaaggcg acctccgtgg tcgacgacca
    10021 ggctgtcgaa acatcgcgac cgccctccgc cgagccggcg cgttccacgc cgagggagtc
    10081 cgcgcggctc tcggagcgcg aacgcgaggt cgccgagctg ctgctgctgg gcatgccgta
    10141 ccgcgacatc ggcaatcagc tgttcatctc cgccaagacc gtcgagcacc atgtggcgcg
    10201 catccggcgc cgactcggcg ccgagtctcg ctcggagctg ttgtcgatgc tgcgggcgat
    10261 gctggcccct caaacctgag tggttgctgg ttaggacagc cttggtcgcg acgccaccgg
    10321 ccgagatgta actatgagca ggcaaatacg acaagttact gaccagtaac ttggtggaag
    10381 ttactcccga gtaacttcac gagctccggg aggcgccggc gtggacaccc agacagtgat
    10441 caggcttgtg gtcggcctgc tgatgaccgc ggtagtgggc gtgttcgccc tgcgccgcat
    10501 ctggtggctg ttcaccctcg ttcggtccgg tcagccggcc accggtcgca ccgacgacct
    10561 gggcacgcgg atccggaccc aagtcgaaga ggtcttcggg cagacgcggc tgctgcgctg
    10621 gtcgatcccc ggcctcgccc acttcttcac gatgtggggc ttctttatcc tgctcacggt
    10681 ctacatcgag gcctacggtg tgctcttcag cccgaagttc gccattcctt tcatcggcca
    10741 ctggcccgca ctgggcttcg tgcaggactt cttcgcgctc gcggtgcttc tcggcatcac
    10801 caccttcgcg atcatccggc tgcggaccgg acccaaagag cacggacgag agtcacgctt
    10861 ctacggctca cacaccggcg gcgcctggct catcctgttc atgatcttca acgtcatctg
    10921 gacctacgcg ctgttccgcg ggtcgtcggt caacaacggc aacctgccct acaactgggc
    10981 ggcgttcttc tcgcacggca tgggcaaggc gctggaatgg ctgggcccca ccggcaacga
    11041 gtacctggag acggtctcgc tgctgctgca catcggcgtg atgctggcct tcctgatcat
    11101 cgtgctgcac tccaagcacc tgcacatctt cctggcgccg atcaacgtga cgttcaagcg
    11161 gctgcccgac gccaccggcc cgctgctgcc gatggagtac gacggcaagc ccatcgactt
    11221 cgaggatccc gacgagaacg cggtgttcgg ccgcggcaag atcgaggact tcacctggaa
    11281 gggcatgctc gacttcgcga cgtgtaccga gtgcgggcgc tgccagtcgc agtgcccggc
    11341 gtggaacacc ggcaagccgc tgtcgccgaa gctcgtgatc atggatctgc gcgaccactg
    11401 gatggcgaag gcgccgtacc tcctcggcaa ggatccaggt ccgctggaag gtctcgacct
    11461 ggagaccgag catgaagaag cccatcacgt tcccgaatcc ggattcggcc gcgtgcccgg
    11521 atcggggccc gagcaggtgg cgcgtccgct ggtcggcacc gcggagcagg gcggcgtgat
    11581 cgaccccgac gtgctgtggt cgtgcaccac gtgcggcgcg tgcgtcgagc aatgcccggt
    11641 ggacatcgag cacatcgacc acatcgtcga catgcgccgc taccaggtga tgatggagtc
    11701 ggagttcccg tccgagttgt cggtgctgtt caaaaacctc gagaccaagt cgaatccgtg
    11761 gggccaaaac gcgaaggacc gactcaactg gatcaacgag gtcgagttcg acgtgccggt
    11821 gtacggcgtg gacgccgaga gcttcgacgg ctacgagtac ctgttctggg taggctgcgc
    11881 cggcgcgttc gaggaccgcg cgaagaagac gacgaaggcg gtcgcggagc tgctgtcgat
    11941 cgccggcgtc aagtacctgg tgctgggtgc cgaggagacc tgcaacggcg actcggcccg
    12001 ccgctcgggc aacgagttcc tgttccagca gctggcacag gtggcggtcg agacgctgaa
    12061 cggcgtgttc gagggtgtcg aaaccgtcga ccgcaagatc gtcgtcacct gcccgcactg
    12121 cttcaacacg atcgggcgcg agtacaagca gctcggcgcg aacttcaccg tgctgcacca
    12181 cacgcagctc ctcaaccggc tggtgcgcga aaagaagctt gttccggtgt caccgatgaa
    12241 ctccgacgtc acctaccacg acccgtgcta cctgggccgg cacaacaagg tctacgaggc
    12301 gccgcgcgag ctgatcggcg cgtcgggcgt gacgctgaag gagatgccgc ggcacgctga
    12361 gaccggtctg tgctgcggcg cgggcggcgc ccggatgtgg atggaagagc acatcggcaa
    12421 gcgggtgaac cacgagcgcg tcgacgaggc gttgatgacg gtgtcgaacg gggcgaagat
    12481 cgccaccggc tgcccgttct gccgggtgat gatgaccgac ggtttgaccg accggcagga
    12541 gtccggcaag ggcgagggcg tcgaggtgct cgacgtcgcc cagctgctgc tgggggcgct
    12601 cgacaccagt acggtcacgt tgccgccgaa gaagaagccc gaggagaagg cggcgcccgc
    12661 accggctgct gccgcggcgc cctcggcacc gcctgccgag aagaccgcgc ccgccgccca
    12721 gcaggaggcc aagccggtca ccgggttggg catggcgggc ggcgcgaagc ggccaggggc
    12781 gaagaagtcc gatgccaccg ccgaatccgc tcaggccgca cccgccaagg gtctcggact
    12841 ggcggcaggc gccaagcgac cgggagccaa gaaagcggcg cccgcagccg aggcgtctgt
    12901 gacggccgct gaaccggcgc ccaccgccac tgagccagcg tcaaccgcgg ccgaaccggc
    12961 atccaccgcc gcagaaaagc ccgtcaaggg gctcggcatc gccgcgggcg ccaggcgacc
    13021 cggcgcgaaa aagtccactc cagctgcagc acccgcgcca gcagaaccca aacccgaagc
    13081 ggcgcaaccg gagccgacgc cgacaccgcc accagcagac gacggtgacg gcgccggcag
    13141 cgcgtccgct cccccggtca agggcctcgg catcgcccgg ggcgcgcgac cgcccggcaa
    13201 gcggtagcag ccacgccaag aatccgccaa gcccttcgcg cgacgatctg cgcaagtttt
    13261 gctgcgtgga agtcggaagc gaggccaccg tggacaccca gggcatcggt caggcgatga
    13321 tcgaacacta cgccgagttg cgacgtcagc gactcttcca tgcccacggc ggagactacg
    13381 tgctacgtcg tgattcagcg gcaggccgat tccacgtgta cttgcgcgca cgcggttcgt
    13441 cccccgacgt cgtcgaattt cgtgcgcgct ctttcacgcg ctttccacac gccgagcgca
    13501 cccggcttct cgaatgggtg aaccgcttca acgaccgcat ccagtggttg ccggcgagcg
    13561 tccgcaattc gcgttactcg gcggatctgg cggtcgttgg caacagccgg ttctgcgtgg
    13621 ccggcgcggg cgatttcgcc gcgtttgtcc ggttcgccga cctgttcctc gccgtcgccg
    13681 cgaagctctt cgaaggcgtc gacgcggaga tgaagctgcc cactcccgcc gagcttgaac
    13741 tgtggtttca acgtagtggc tagagcctgc tggcgcggtg ggcccctgcg atccgtgcca
    13801 agaatcctcc aagtgcctcg cgggatcgtg ttcgcgtgac tacccgagga ctcggtcagt
    13861 tgatgatcga acgctatgcc aagaagcgca gtgagcggct tttccgtgcc aacgacggcg
    13921 aatatgtgct acttcaggac tcagcccgag gccactttcc cgtgtacatg cacgcccgcg
    13981 gttcgtcccc cgacgtcgtc acacttcgtg cgcactggtc ggcgcggtgt gcgcacacca
    14041 agcgcatcca ccttcgtgag tgcgtgaacc gcttcaatga tcgcaacccg tggttaacgg
    14101 tgagtgttcg cgaagcgcat gactcttcgg ccctcacagt cgtcgtcaac agttcgatcc
    14161 tcctagcgga cgaagtcgat ttcggggtgt ttgtccggtt cgtcgacctg tcactcgcct
    14221 cggccgccaa gttgttcgaa caggtctacc cagggacgca gctgccctct ccagctgagc
    14281 tcaggcggtg tctgcaactc cccgcctgag ccagcaaacc gcggactttc gaaattcagt
    14341 ggacaaagct ggcaccatgg ggacgtgacc acccaccagc tgccgtggca tgccaccggt
    14401 catcaccatc cgcagcgcac gtttgcgcag tcgacgaagc tgcaggacgt cctgtacgag
    14461 atccgcgggc cggtgcacga ccacgcggcg cggctggagg ccgagggtca tcgcattctg
    14521 aagctgaaca tcgggaatcc ggccccgttc ggcttcgaag cgcccgacgt gatcatgcgc
    14581 gacatgatcc aggcattgcc gtacgcacag ggctactcgg actccaaggg catcttgccg
    14641 gcgcgccgcg ccgtggtgac gcgctacgaa ctcgtcgacg gcttcccgcg cttcgacgtc
    14701 gacgacgtct acctcggcaa cggggtgtcc gagctgatca ccatgacgct gcaggcgctg
    14761 ctggacaacg gcgaccaagt gctcattccg gcgcccgact acccgctgtg gaccgcgtcg
    14821 acgtcgctgg ccggcggcac gccggtgcac tacctgtgcg acgagacgca gggctggcag
    14881 cccgacgtcg ccgacatcga gtcgaagatc accgaacgca ccaaggcgct cgtcgtcatc
    14941 aacccgaaca atccgaccgg cgcggtgtac agccgcgaag tgctggagca gatggccgat
    15001 ttggcccgca agcaccagct gctgctgctc gccgacgaaa tctacgacaa gatcctgtac
    15061 gacgacgcca agcacgtctg cgtggcgtcc gtggcgccag acctgttgtg tctcacgttc
    15121 aatgggctgt cgaaggctta ccgcgtcgcc gggtaccgcg ccggttggct ggcgatcacc
    15181 gggccgaagg aacacgcgag cagcttcctg gagggcatca gcttgttggc caacatgcgg
    15241 ctgtgcccca atgtgcctgc gcagcacgcc attcaggtcg cactgggcgg tcaccagagc
    15301 atcgaggacc tggtcttgcc gggcgggcgt ttgctcgagc aacgcgacgt ggcgtggaac
    15361 aagctgaacg agatccccgg tgtgtcgtgc gtcaagcccc aaggcgcgct gtacacgttc
    15421 ccccggctgg atcccgaggt gtacgacatc gtcgacgacg agcagctggt gctcgatctg
    15481 ctactgcaag agaagatcct ggtgacccag ggcaccggct tcaactggcc cgcgcccgac
    15541 catctgcgca tcgtcacgct gccgtgggcg cgggatctcg ccgcggccat cgagcgcctc
    15601 ggcaatttcc ttgccagcta ccggcaataa ccgctacttc tcgtcgagca actcgagcag
    15661 gtaaccgccg tagcccgact tcagtagctc gttcgcgcgc gccgccagct gctcgtcgtc
    15721 gatgaggcct acgcgccagg ccacctcctc cggcacgctg atcttcaatc cttgccgccg
    15781 ttcgatggtg cgcacgtagt cgctggcgtc cagcagcgaa tcgaaggtcc cggtgtccag
    15841 ccacgcggta ccacgggtga gcacctcgac ggacagctgc ccgcggttca ggtaggtctg
    15901 gttgatctcg gtgatctcgt attcgcctcg cgccgaaggc cgcaacgacc gcgcgatctc
    15961 caccacctcg ttgtcgtaga agtacaaccc cggcaccgca tagtgcgact tcggcgtcgc
    16021 cggtttctcc tccaacgaca gcggcgtgcc gtcggcgccg aactccacca ctccatacgc
    16081 cgacgggttg gccacccagt acgcgaaaat cgctccgccg ctgacgtttt gaatccggct
    16141 gagattgctt ccgagaccgg gtccgtaaaa gatgttgtcg cccaacacaa gtgctacgga
    16201 gtcggtgccg atgtggtcgg cgccgatcac gaatgcctgc gccaacccgt cgggcttctc
    16261 ctgaacggcg tagctgatgt cgatgccgaa ggccgagccg tcaccgagca gcctttcgaa
    16321 cgaagcggtg tcggcaggtg tggtgatcac caggatgtcg cggatgccgg ccatgatcag
    16381 cgtggacagc gggtagtaga tcagcggctt gtcgtagacg ggcagcagct gcttgctcac
    16441 gcccatcgtg atcggataca gccgcgtgcc cgagccaccc gccaggatga tgccgcgcat
    16501 gtatgcccct acccgacgaa gatgtcgtct tcgctgagct cggtggcggc caacgccgtt
    16561 tccaggtcat cgtggcggaa caccgaggtc accttgtcgt gcacgacgcg aaacgccgac
    16621 gccatcactg ttcgctcttc gcgcaaccgc tcatcgccga cttgcggcga tccggcgtcg
    16681 ccgatgtgct gctcgaccac cacgacgccg tcgtggacgt acattcgtcc gggctcgatg
    16741 agaaacccct tcgatgtcgc ccactcgcgc aacgccccgt gaccttgcac cgcgccgtgc
    16801 gcgtcgccga tctcgatgtc cccagtggac agctcgacca gcgtgtcgac gtcctgcgtg
    16861 ttgagcgcgt cgtgccatgc cagcacggtc gcaatctccg aaacggtcat gagtgaaagc
    16921 tagtacctca gaacggggtg cggtcgagcc agccccgcca cacgtcgccg tcgccgaata
    16981 cggtgagccg tgggtcgtcg gcagggatgc gccgggtcat cgcaagaagc agcgacgcgg
    17041 catcgccgcg cacggccacg gtcgacttct catggtgccg agccagctgc aggttctcgc
    17101 cgtcgctgcg cagcgtccac tcgccgccgt cgctggcatg cagatgcagt gactgcccat
    17161 cagccagcgg catgcgctcg ccgccggcct ggatcacaac ccattccagc cactcggtca
    17221 ggccgtcggc cgcgaggtcg gccgagagcc ggaagtctcg cccgagggcg agctcggcgt
    17281 cggcgcggtg gacagtgacc tcgtgcagtc gtcgccggat ccaccacccg gccggccgcg
    17341 gtcccaagaa cgtccacacc ccagtgtcgg caccggtctt gtcgacggcc tcgacgaggc
    17401 ttcgcgcgcc gtcgtgcagc cattggatgg tgccgtcgcc gtcgtcgggc ggcttgccgc
    17461 cgcggacgtc gcgcgggtcg aggaagtcgg tgagccggtc gccgacgatc tgcgcggccc
    17521 acctgtcgcc gcgcccgacg tggcgcgtca gttgccgcag tgtccagccc gggcaggtcg
    17581 gcacgggtgt ggccggatcg gcaccgcgca acacctccgc gaacgcggcg ttctcgtcta
    17641 tcagcgctgc ggcgaagtcc acgtcatgcc tccaatgtga ccggcagtga cgaccagccg
    17701 cgcagcacgc gggtgtcgcg tcgggtgccc tcgccggcga gacgggcgtc cggatagcgg
    17761 tcgaagaatg accgcagtcc cacctcaccc tcggcgcggg ccaatgcggc accgaggcag
    17821 aagtgcctgc cgctggagaa cgacagatgc ttgtttgcgt tgggccgctc gatgtcgaag
    17881 cggtgcggat cctcgaagac ggcggggtcg cggttggcgc cggccagata cagcaccacc
    17941 atctcgccgc ggcggatcgt cgtaccggcc acgtcgatgt cgcaccgtgc cacccgcgcg
    18001 ctcaactgca ccggcgagtc gaaccgcagg atttcctcga cggcgttcgg ccacagctcg
    18061 gggcgtttgg acaacgtgac caggtgctgc gggtgctcca gcagcatgcg aatcccgttg
    18121 cccagcaggt tgaccgtggt ttcgaagccc gcggccagca ccaagcccgc ggtagcctgc
    18181 agttcgcggt cgttgagccg accgccttcg tcggcggcct gagtgagctg gctcaacagg
    18241 tcgtcgccgg ggtttcgacg cagctgccgc agatgttccg ccagccacgc gttgaacccg
    18301 acgatccctt gattcacctg ttggtactgc cgccaggaca gcccgatgtc caggctcggt
    18361 gcacccagct cgccgaaatg aaggatctgc gcgcggtcct cgtcgggcac gccgaggatg
    18421 tcgccgatta tggtgaccgg cagctgtgag cagtagcgcg ccacgatgtc gacggcgccg
    18481 gacccgtcac cgaggtcgtc gagcagcacc ttggcggtct cttcgacgcg atcgcgcaac
    18541 gcggccaccg cccgggtggt gaacaccgac gagacgagct tgcgatagcg ggtgtgctcg
    18601 ggtggttcga cggacagcag cgacggcggc gcgagtgggt gcagcaggcc ggtgtcggtc
    18661 ttttcggtca cccagcgcag cggcttcggc agattgccgc ccagcgcggt gacccggaag
    18721 tcctccgagc gcaggagttc gtgggcgatc tcgtagtcga ccgtcatgta gaccacgcgg
    18781 cctctgacga tccggcccat ggcgcgcagc tcgtcgatga acggcatcgg gttcgcgcgc
    18841 accgccggat cgaagatcat ccgggcctgc ggctcaccgc gacgcattcc gatcgatgcc
    18901 agcccgcgca cgacgccgtg caaggcgagc caacgaatcc tctgcctcat gagtcgagct
    18961 taagaactgg tcgcagatcg tccaaccggt cgaacaactg ccagacgtag tccgacgatc
    19021 cgttctcgcg gtgcgggtga aggtcggcca gcgcggggtc gttgcaatag ccgtcgaacg
    19081 ccgcctcgga ttcccactcc acgaggatca gcacctgcct gggtgcgacg tcacccgtga
    19141 tcgcccgccg gaacttcccg agcgcgacga ccttgccgcc gtgggcggtg acctcggcga
    19201 cggatcgccg gctgtatgcc aggtactcgt cgcggtcggc gacgttgaac aggttcagcg
    19261 cgtacaccgt catgtgacgt cacgctacgc ggcgaggccg tctccccagt gcgtaaactt
    19321 gccatccggc agtggtccag cgcgccacgt cgaggcaatt gcggccgtcc acaacgactc
    19381 tcgcgcgtac cgtgccgacc agctcgtcgg gatcgaggtc gacgaactcg cgccactcgg
    19441 tgagcaccag caccgcgtct gcgcggtcgc acgcctcggt caccgacgtc gagtaattca
    19501 gcgtcgggaa gacccgttgt gagttctcgt ttgccttggg gtcgtagacg ttgacggccg
    19561 caccgttgag ttgcagcatg ccggcgacgt tgagcgccgg cgagtcgcgc acgtcgtcgg
    19621 actcgggttt gaacgccgca ccgagaaccg cgacgttggc accgagcagc gaaccgccgc
    19681 aggccctggc cgccagttcc accatcttgg tgcgccgccg catgttgatg ctgtcgacct
    19741 ccctgaggaa cgtcagcgct tggctggcgc cgagttcgcc ggcacgtgcc atgaatgcgc
    19801 gaatgtcctt cgggagacag ccaccgccga acccgaggcc cgcgttgagg aaccggcgtc
    19861 cgatgcgcgc gtcgtagccc aatgcgtcgg cgagcacgct gacgtcggcg tcggcggcct
    19921 cgcacacctc ggatatcgcg ttgatgaacg aaatcttggt tgccaaaaag gcattcgcgg
    19981 acaccttgac gagctcggcg gtttgcaggt ccgtgcacaa gaacggcacg ccggcatcga
    20041 gcagttgagc gtaaagatcc cggaccgccc tctccgcgcg ctgggaattc gcctgtacgc
    20101 caagcacaat gcggtcaggg tgcaacgtgt cgtggaccgc gaagccctcg cggaggaact
    20161 cggggttcca tgccacttcc acggccacgc cgtcgcgctg taacgctgct gcgcgcgtgg
    20221 ccaactcggc cgcagtgccc accgggacgg tcgacttgcc gacgatcacc gcgggacgtc
    20281 gtagccgcgg aaccagagcg tcgatcacag cgtgcacatg ccgaagatcg gcgccgtact
    20341 cgcctttctt ctgcggggtg ccgacaccca ggaagtgcac gtcggcgaaa tcggccgcta
    20401 cgtcgtagtc ggtggtgaac cgcaatcgtc cggcggagat gttgtcgcgc aacatctttc
    20461 gcagaccggg ctcgtagaat ggcgcttcac cggccgaaag cttggccacc ttgccgggat
    20521 cgatgtccac accgacgacc tcgtggccga gctcggccat gcctgcggca tgggttgcgc
    20581 cgaggtagcc cgtgccgaag acggtgcacc gcataccgcc ggtatagcgc cacgcgatga
    20641 gccaaccgcg tcctgacact gagcggcagg acaacatcag gttacgtatc agtcccagta
    20701 ttcccagaac ggagtgcggt taccgccgag agaaggtagc tgcggcagct gaatcaccgg
    20761 ctgccgcggc tgttgcgtga tcaccggctg ctgaggcacg acaggctgct ggggcaccgg
    20821 ctgccgcgga atcagcgtca cctgtggctc gtctggctcg tcgtagaccg gcggtcggta
    20881 tggcggagtc gacggcggct gctgcggttg agtcgtcggc ggctgccacg gtggcgtcca
    20941 ttgcggctgc tgcgggtacc acgggtattg aggtgattgg taagacggct gcgggaacag
    21001 ctggatgatc ggcggaatga tcaccggcgg cgcgatcacc ggcgtgggtg cggccgggac
    21061 cggtgccggc ggaggggcgg ccggtgcggg aggcggagca gctgcgggaa cctccgcagg
    21121 cgcctgcgga ggttcgacga acacctgccg cggggcctgc tgcggctgct cctgaaccac
    21181 gggcacgggt gccggaatgg tttccggtgc cttcggcggc tccgcgggtg gcggctcgac
    21241 ctgctgagcg gcgggagggg cgggtgccga ggtgctcggc acgacgatgc tctcgcccgg
    21301 gttgggacgc tgatccaccg tcggcctgat gctgacggcc agggagatga ccagggccac
    21361 tactccgacg acgaagatgc tcgtcagggt actgcccacg agcatgaacg gccgtcggct
    21421 ctcgtcggcg accacgtcgt cgtcgtcgtc aactggcgag gccgcactcg tcggcctcat
    21481 ctgcgtggct gcgaacgcgg cgggatcggg gtcttgcgag tacgccagcc cgatcgtcgt
    21541 cgcctcgaat cgcggtgcgc tcacagcagc cagcgccgct ccgcgggcca gggccatctc
    21601 ggagtcctcg ggcgcgctga cgggaagcga caccaagttc tcgaggtgct ccttgaccga
    21661 ggcgacgtcg acgccggaac cgacgacgaa cacgccgccc ggccgcacgt cctgggcctc
    21721 caggctggtg accatctcgg ccatgaccgc catcgcgtcg gcgctgtgca ggctgcggct
    21781 cacgaccttg acgatcgagc cgtcgtgggc ctcgaccacc gacaacgtcg cggtgttgcg
    21841 ctcgacgaac atcagcgccg tcttgtcgta gccgaccgcg cgacccgcgg cctgagcaat
    21901 ggcgcccgcc gcgtgcaact ccgacacaag catgacctcg tcgaggccgc gggcggtcag
    21961 tgcgtcgcgc agcgcgctgg cgtcgtcgtg attggtccat gtcacgccgg tggtgatcag
    22021 ccggtgtccg ccggcggccg cgctctgctg cgtccccagg atggccgaga tcacctggtc
    22081 ggacgcgctt gagtttgctg cgccgtcgac tgccgtgatg tcgaagatgt cgtggtcgac
    22141 ggtgcgaccg tcggccttct cgccttcaac cagcaccatg cggaccgtcg tgggtgtcat
    22201 cgacacacca agtacgatgt ccacagcccc tccatttagt ttgctagtta gccctggccc
    22261 ataggggcgt tgtccagcaa aaagtccgtc aactagtggc ttcatcgcac gatcagcccg
    22321 atgcgttaca tcccccgacg ttagctgatc ttgatgatgt caccggtggg cggcgcttgc
    22381 tgggctcctc tggacctgcc cctcaaacct tactcgcaga cgacgcggtt agcgccaatt
    22441 atgttcgcgg cgacggcccg atgaagttct ggtacgagcg cgacggagtg gggccgcgct
    22501 ggccctggta cttggatccg accttggcgc tgccgtacgg atgctcggct gggctggtca
    22561 gccggagcag gcacagctga ccgatcttca ttcctggcca caacgtgatc ggcaggttcg
    22621 caacgttgga cagctcgagg gtgatgtggc cgctgaaacc ggggtcgatg aagccggccg
    22681 tcgaatgggt gagcagaccg agccggccca gcgacgactt cccctccagc cggccggcga
    22741 ggtcgtctgg gagcgtgcag agctcaagcg tggagccgag gacgaactcg cccggatgca
    22801 gaacgaacgg ttcgccggac agcggctcga cgaggctggt gagctcgtcc tgctgcaggg
    22861 cggggtcgat gtgggtgtag cgggtgttgt tgaaaactct gaacaggctg tcgaggcgga
    22921 cgtccacgct ggacggttgc accagcttgt cgtcgaacgg ctcgaggcca aggcgtcctg
    22981 cggcgatttc ggcgcggatg tcgcggtcgg agagcaacac ccgtcgagcg tatcggccga
    23041 gcccagcctc ggcgggttgc tatccttcgt gatcaccagt gccgatgtag ttcaatggca
    23101 gaacatcagc ttcccaagct gaatacgcgg gttcgattcc cgtcatcggc tcaggaaagc
    23161 cgcacttcca cggcttgcga aggcatcaac acgacgagtc gacggcgttc ttggccgtcg
    23221 ttcgacgatg atttcgcgag gcatcggtat acgaaccgtt tgctatctcg gtgcgtggtg
    23281 gaccccttag cctgcgcata aagaatccgc caagacattt gttggacgct ctgctccatg
    23341 cagcgtccga atattgggtc aggcgtcgcc acaaccgcga tcgcggtcgg cgtcgtgctg
    23401 atagctggtc cgtacttaag ggagcggccg ggcgccggcc gcggcacaag accatggacc
    23461 gcatcatgaa cacgatcgac ctcgtcatcg tcgcccttgg ctgcggagtt ttcgctcggg
    23521 cgttggcacg gtacgcgccg atagcggtgc cgggagcagg cctggcggcg cagggcgtgc
    23581 tcggcgtgta caccgggctg atggtgcgca gcatctcatt cgccgcgctg ggctcgaact
    23641 ggccgattgt cgtcgctgtc gcggtcggca ccctggtcat cagcgtcgcg ggcggcgcgc
    23701 tgctaggtct gcaccgtgac gtcagcccgt tgacgggtgg gctcgcgttg gtcgcgggag
    23761 gctcgtccgg actggtcgcc atcgcccgcg aactcggtgg tgacgatcgt gtcgtcgcgg
    23821 ccgttcaata cctccgcgtt gcgctgatca cggctgccat gcccgtggtc gtgacggttt
    23881 tctaccacgc caccggggcg catggttcgc agattagcga aaccacttct ctcccttggt
    23941 atttcggcct gccgctgatc gcggtcattg ttgtggtcgg cgcggcggcc ggccgctctg
    24001 tacggctgcc cggatccggg ttgctaggtc cgatggcgat caccatcgtt tttgaactct
    24061 ctggactaac ggcgggtctc acggttccga tggtgctggt ccaggccgga atcatgctga
    24121 tcgtttggca gacggtgctt gcgttcaacc gagagtcgct tcgggcgatc caacggatcc
    24181 tgcccggcgc gttcgcgttg atcatggtgc tgaacgtcgc tgcggccggt ctcggcgtgg
    24241 tgctggccaa tgtggccgga ctgagcatgc tcgacggata ccttgcgacg agccccggcg
    24301 gcatttacgc cgtgcttggc accgctgtgg ggtcgggctc caacgtgccg tttgtcatgg
    24361 cggcgcaagt cattcggatt ctactgatgc tgttcgccgc gccctttgtc gcacggttat
    24421 tcatgagatt cacgcctcaa agcgcggcga gcagagagct catccctgtc gccgcttgaa
    24481 cgccgaccgc cgccacctcg ttgtgtctga tcgacgctgg ctgatcagcc aaccccgcgt
    24541 caccaccgtc accgagctgc aaaatccaaa tcgacgcgtt cgtcgacgaa tacaaccgcc
    24601 accgcctgga caccgcccgg ccaaggcggc agggccggaa ccgctactgg gtggtgccga
    24661 cggccgatca ctatcttcaa tgcgatgctc cgcagcagtt agcgcagatg gttcgactgg
    24721 cagcggacac aggcgatatt tcgctccagt cactcgggaa caggtccgac agagcggtcc
    24781 ttgtggatca ggagagcagc ggatgaagcg ggtcagtagt cggcgaccgc cttctttacg
    24841 acggcgtcga cttcttccgt cagcaaaaat ctctcggcga caagcgattc ggcggccgcc
    24901 cgaactgcgg ctgaataatc atcgcgtgtc ggataacgct ccgtggccga cggccgcccc
    24961 ggtggaaatg gcaacttgct gccgattcgc tcataaagca catcgggcag ctcgtccatg
    25021 tgtcgcctgg gattccagcc cgtgtaggtg gcgagcggtg cagcgaccgc aggcaggcgt
    25081 atgccggcga tttcgttgcc gtccgcgtcg atcgccgaca ccaagtcaac gtacgtctcg
    25141 cccagcttca cgggccagtg gccgataccg cgatcgacgt ccggcccaag gttgacgctg
    25201 cgcgcgtagg aaagcgatgc gggatccggc gcggcggtgt ggccgaagcc ggacagcacg
    25261 tccttgcggg agacggcagt gcccttgctg gtccgcggca cctggctggg cgggggttcg
    25321 acgccgtcgg tgacccagtt ctccagcgcg accagcagtg cccgattcac gggcgtgacg
    25381 tccaggcggt gcaccgggtt cgcggccggc agcgcctctt tgatgcggct gctgccgaag
    25441 tggtcggtac ctgcgatcag ataggtgcgg acgtcgggat cctcaggcaa gtccttacct
    25501 gtgtacgggt ccacgtgcag aagcgcaccg tcgccgtgcc agtattcgga tgcgctgttg
    25561 gtgagaatcc gcccttcagc caaggtgcat aggtgggcag gccgcggttg ggcacaacga
    25621 acagcatttt gccgttgccc ccgtcgatcg ggcgaagcat gcggaggtca gcgtcaaagc
    25681 gcacgagtcc gtcgtcgtgg cgaggcgcga gtttgaggtc ggcaatgcgc tgatttgcga
    25741 gatgctcggg gtcgacagcg aagcatacgg tcgcctccac gcactcatag cggtcatcgg
    25801 cgtgcgggta gaccgtgttt atctgaactg actcaacggt cacgtcagct ccttcaaggt
    25861 tgcagttcgg ttagtcggca gtgccgatgt ggtcggtggc tcccatcaag gttgcggcag
    25921 acgccttggc aaattcttgg cgactgtcgc ggcagcccca gacgtcccgc agccgcttca
    25981 atggccgcaa ctcgtctcgc aaatcgcctg cgcgcaaagt tatttcgcga tggccgcggc
    26041 gtaagcacgg cccgcgtgca gctgctgggg tatgtcgtgc tgcacattga cgcgtgggcc
    26101 acgctggggc ccatgagcgg tgggcacgtg taaccggagt atccggccgc cgatctggac
    26161 gtacgcgccg gggcgccatt tcgcagactg ccagggggcg agcctggtcc agccctgctg
    26221 tgccggttcg cgcacgaaga ccccgttggt cgagttgcgg tcgacgatga tcacctcgcc
    26281 gttgacgatt cggacttcta cgtgggcccg ggacatctca cccgtgcggt catcgatacg
    26341 tacggggcga agtccgcgct tcgcagcatc cgacccgtac ggatcccggc cgatgacgca
    26401 gtctcgttct atgtcgaatc gagtgttgtc gtcgaggaca acccatcgct tcggccgcac
    26461 gaactcgacc gccgctggtg tgggaatcct gtcgccacga gcgggcgacg gcatcgctgg
    26521 cgcgcgcctc ggtgccggcg atgcctgcgt cgaccagacg acggcgcctt gcgccggcac
    26581 ggttcctccg gtgagcttgt acacgccgtt ccgctgggcc ggggcgagcg gccgctggct
    26641 cacttcgtcg acggtgacga tagctgcaac gcccggtacc ggcacggatc ggtgcaataa
    26701 ccgtccccgg ccgtgcaata cggtccgccc gacgccgtca tcgagcacta cggtgacact
    26761 gccgcacagc aagacgtcca ggcctgcggg agtgggcatg atgatgccga cgtcaacaaa
    26821 gatgcggctc cacgttgttt gacgcgtgag ctgcgaaaat gcgcttgtca accttcgcgc
    26881 gtcggtatgc cggaccattt cggccagtgc cgccaactgc tgtgcggcaa cagactgctg
    26941 cgagagcggc gccctatcgc gttgggccac aacgataacc gtgccgttga cattggccac
    27001 gagatgccgg ccagggatga cctcgacctc gcttggcggc ctgaccggca tgtggcagac
    27061 caccgttttt tcgtcggcca cttgcttcgc aatttggtgc ggcatctctt caggtgtgtg
    27121 tggcactggc ggagcggttt cgtcgtcgga cggggcttcg ctgatgccga tgcgggagtg
    27181 cagccacctc agcggccgtg gcgcccacca gttgaggtcg ccggcgacac gaagaaacgc
    27241 cggtacgacg acgccgcgaa tgatcgtcgc atcgatcacg acggccagcg ccgtgccgat
    27301 gccgaacatc ttcatgaagg ccagcccatt ggcaaacgag atcaacgtga tcgtcagcag
    27361 gagcgcggcg ctagtgacga tccggcccac ccggcccagt ccgacgacgg ttgcggcgtt
    27421 gttcgacaac cccgagtcac gggcttcctt tattcggctg agcaggaaga tctcatagtc
    27481 gacggacagg ctgaacgcga tggagcagag caagacgacc atggacaggt tcagcggcgc
    27541 gggagtgatt cccagcaccg aagccaagtg cccttcctgg aagatccaga ccatgacgcc
    27601 gaggaccgcg ctcaggacaa gcagattcag cagaagcgct tttgtcggca caacgacgct
    27661 gcccgtgaac aggaacagca gtatgaacgt ggaaacggcg atcagggcaa tcgccaacgg
    27721 aagccggtcg gcgatcgcgg cgcggctgtc gatgagcgtg gcggtcggac ctcccacctc
    27781 gacctggtgg tcggcgatct tcgcgcggat gtcgtgcacg agatgttgag cgacgtccga
    27841 gtccgcctga accgacaggt agacgaacgc gtagctggcg ccggactctg cggtcgcggg
    27901 cgtttgcccg acgatctggc cgtgttcgta tctgccgatg ggaccgctga ccaggaccac
    27961 gccgtccatg cgcgatacct cggcggcaag atccttgagc acgccggtgt cagtgcgggt
    28021 caccaacgtg atcgcctgcg agggatctag ctgaaagtcc ttctgcagcg attcggacac
    28081 caaacgcgca ttcgagtccg tcggcagcgc gcgctcgtcg ggtgtcgcgt actgggcgct
    28141 gaggaaggga atcccgagtc ccagcagcac tgcgactacc ggcagggcgt agaagagcgg
    28201 ccggcgtgtt accgcctggg cgaaacgtcg ccagaatagg gaatcggccg aaagcggtac
    28261 ctttcggcgg atgaccgcaa gtgagtcgat gcgcttcccg agcagagcca gcagggcggg
    28321 caggacaacg atcgcgctga atgccgacag catgacggtg gcggtcgcgg taatgccgac
    28381 ggatcgcagg aagtaggtgg ggaacacaag gaggccggtc atggccaggg tgactgttgc
    28441 tgcgctgaag aggatggttc ggccggcggt ggtgacggtc gcgatgatgg cctgatggtg
    28501 gtctttcccg ttgtcgcgtt cctcgcggaa tcgcgacacc atcagcaagc cgaagtcgat
    28561 ggacaacccg aggccgaacg cggtcgcaac cgtgagtgcg tgcaccgaca catcggtgat
    28621 cttcgtcatg agcagcaaca ccgacagcgt ggtgacgatc gagctgattc cgacgatcac
    28681 gggtaggaat gcagcgacga gaccaccgaa cacgatgacc aacacggcga gcgagatggg
    28741 cagcgcgatg ctctcgctga gcttgagatc gtgtttgacc ttggcccgga tctcttgctg
    28801 cacgccaaga gagccgcccg cgcgcactgt gacgttcggg tcgtcgggca aacgcgcgat
    28861 gatgcggctg gccgtggctg cggcctcgtc tgcggtccca ccgacgtgca cgagcatgag
    28921 gccggagcga ccgtccttgc tgcgtaagtc ggtggctcct tcgtcaccga acgacctggt
    28981 gacctttgca tccggctcgg cctcgacgag ttgccgtact cgatcccgta ccgtcgcgac
    29041 atcgatgctg tcgacagtgc cctcgcgcgg gagcagttga atgaccagat tcgaggtcgt
    29101 gccgaaattt tggtccagga attcgtcggc ttgcgacgat tccgacgtcg gatcggtgta
    29161 tccgcccacg ccaagcttgt cggacactga gccgccgaac acgatgctga tgccaagcaa
    29221 gaccaagagg gccgccagca cggcctttgg gcgccgcacg gtgtatacgg ccaatcgcct
    29281 caacatgtca gttgcattcc ccactgcatc tcaatcgcct tgttgatctc agaagtgtgt
    29341 cgcgcgggcc ttggcacttt ctaaacagag atcgcagctc gaatcacgcc tgctcaggcg
    29401 gcgcgaaggc gagccgttca attgcgcggc tcccctgcga gccacagccg caccaggcag
    29461 cgccggttgc ccacctcggg cgcgtcgaca aaacggcgcc ggttgtgcag tgtcacgtag
    29521 tcgtcggcga acaagacgtc gccgggatcg agcttgaatt caacggagtg ggcgggatcg
    29581 ttcaggaagc ggtcgaaggc gtcgagcgcc tcgcgctggc gttcaccgag cggccggccg
    29641 gcgagcatgt ggcctagttc gatgtaggcg cggttgtagc ggacgcggac gccgtcggcg
    29701 gtgtcggtga agaccgcgcc gaccgacacc gggtcctcgc ccggtccggt ctccttcgag
    29761 cggtcgaagc agaactcccc gtagagttct tccaccagtt cgggctgcgt ctccagcagc
    29821 gcgttgtagg cggtgtggcc gctgacgatc agcgactcgc cgccggacac cgccttgcgc
    29881 aggcacagca gcccgagcac gtcggcctgg atgccgacgt agccgctggc cgagtccgtg
    29941 tggaagatca gctccgagtt cgtcttcgaa ccgcgtgcct cagccaccga cttgccggtg
    30001 tcctggacca ggtagacgcg gtcaccctgt cggttctgcg gctgcggccc gcctaggtaa
    30061 gtacccaggc cccagtacag catcgacgct tcctcgtccg tccatccctg gaagggcagc
    30121 ccgcgcagca aacagaagcc ggggccggag cggatgcggt ggcggatgcc gcggccgatt
    30181 tccgcgagcg ccgggtcctg gaagtccgac ggctcgatgc tctcgagccg cgtgccgcga
    30241 cgccgcagct cgtcgcggat gcgcaggcaa gcggcggcct gttgcggctc gaccgtgatt
    30301 atgtagtgct cggcgcgaag gctggcgtga ttccagaccc ccgggccggc catcggccgg
    30361 cgccgggtgc cggacttttc ttgcgtcgcc gcgttgctca tttgctctgt cctttcacta
    30421 aagtcatgag catctccgtt cacccccttc gtctggctgg ggtgcgttta gtgagcttca
    30481 cggccgcggc ttggaaatct cttggagcca cgcaccagcg ggcccgccgc cgggcagttg
    30541 gacggcgggc taagtgaacg gaagaaactc ttcagacgcg ctgcggcgtc ctccgtgtgc
    30601 cgtcgacttg agtccgcgaa gtcggcgtac accacatgag gaccggcgag aacgcggata
    30661 gcacggcgtc tgcctcacaa cccacattat cgtgtctgac ccgaaaatct gaatgcgtta
    30721 caataaaacc gcagctcact gactggcatc ggcgcgattc gccccttgtg cgcgccaggg
    30781 ccttgaaatc gagggcgagc gagcacgccc gattttcccg catctgcggc gtccccgaag
    30841 ggaaccggaa aactgacatg gaggccgtgc ccggtcggtg attcggtcgc tgatctgcgg
    30901 atttacgggg gtatcgctgc cggttcccgt ttcggcgctc aataataaat agagcttttc
    30961 aggtgattct ggcgcgggtg aagcgaggca gccaggcctg gcgcaccgct acagcgtcaa
    31021 atcccaccga ccaccgcaac acccattact gagctatgtc gggagtcctg atgaccggcg
    31081 cagccgcgcg gtgtcgatcc ggcctgcgga cagactgtcg tagagcgcgc cgtgcccgcg
    31141 ccggtgctcg gcggccagcg acaactcgac cagcgagccc acggggccgt cggcgcacag
    31201 caccgcgtcg g
//
LOCUS       DASWBF010000385        11036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_819480,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000385 DASWBF010000000
VERSION     DASWBF010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819480"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VF874_14355"
     CDS             complement(<1..708)
                     /locus_tag="VF874_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEX9177200.1"
                     /translation="MSDRRLKIVVFAVAVVGYLAVGYRLQVDHGFILGDALSRVSAAQ
                     SVLFSRDPHLAAIGFIFTPLTAMVQLPWIAMSPLWPAMTERAFSGTIMSSIFMAGAVI
                     QILGVGTDRGLPRWYSLSIVMLFALNPMIVFYGSNGMSEAPFIFFLAWAVRRLVMWMV
                     DDDVHHLVAAGGIAMGLAYLTRYDAVACIAAAGILVGATTYLRARPPPRLRRALLDML
                     LVSGPGFAAFVGWAVASW"
     gene            complement(720..2195)
                     /locus_tag="VF874_14360"
     CDS             complement(720..2195)
                     /locus_tag="VF874_14360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010842063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9177201.1"
                     /translation="MTAAFRSDDLHDVTDAEREQALRAAIEGLREDDPIHSAAKAVMG
                     WQRPVLATILVATVALAILRPMGTAVTLVGLCTFGYVCTMIDRVLIFRRGLAARPIVV
                     TDEEARGIPDADLPPYTVLVPAYNEPEVVADLIGAMAALEYPREKLQVLLLLEADDDV
                     TITAAQRCQESDAITIVLVPPADPRTKPKACNYGLQFATGEIITIYDAEDKPEPLQLR
                     RVVAAFHALPDSVACIQAKLAYHNGHQNLLTGWFTAEYGLWFGYLLPGLMRTNSPIPL
                     GGTSNHLLHPVLDKIGAWDPFNVTEDADLGLRIAATGYQTAVVDSVTMEEANSDPINW
                     IRQRSRWYKGYLQTWLVHMRRPGRLLRTIGWRSFIRFNLVVGGTPIIAVLNLAFWLIT
                     LLWFLGQPAAIGAVFPWYIYFPALAALILGNAATLYMNMIALREDDRSDLLIPALTVP
                     LFWLMMSIAAAKGLYQLIRNPSYWEKTFHGLDRPENQKTAT"
     gene            complement(2192..3706)
                     /locus_tag="VF874_14365"
     CDS             complement(2192..3706)
                     /locus_tag="VF874_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177202.1"
                     /translation="MTTAVQPAAPDTTARREPWLHLLQPGYRVALRWLYIGVLTLIAF
                     RHSLASLVEVTRGGGMGGYVWIVPLAAVLAGTSVARRPRTELPIHDRQTDIIVGTLGL
                     VLALLVHAVLMQRYALYFHLLRLDFLAMWLFVVGAAVVLFGLRPVTRFFWVWMLLLMV
                     FPLPYHLTVFLLGGNRMAGGAATLIVGAFATAIAVGRSVRRAVIGSILAWAVGLGVLA
                     VMALYLPDAPLLAYQQIPALTAILLVGILLYLHARRGKEKNFLDRKIEPLAAGQVWVG
                     LPLVAVFAILLSLVKLPTPPTPPTTQISEMNFAAPLPAPAGWKIIDEQTFPWVHRLYG
                     RDAEVTRQRMIALSGNPKWDKHSDPRTVVVDSVVTGRPMSFVVYPTTLLYDLSATRLS
                     APRRVDLGHGVTGQLVSVVDDKLVVTWNALSWTWRNARQAQRIVVLSVDNHEDDAPFP
                     IPAGGIPGTLNSMFTILFRGNAAVGDRNPVFKDAEMLTEFSRALVAARMRGFGP"
     gene            complement(3708..5654)
                     /locus_tag="VF874_14370"
     CDS             complement(3708..5654)
                     /locus_tag="VF874_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177203.1"
                     /translation="MNAAATGWLLRAWVIALVLTMGWIPAVANSAPGDGGLPAESPTL
                     SLESLGVDSTFAFYGVQGTQNLTVPVPRGLAPTALNVVVEMPPNLRSGVIAVTQDNRV
                     VSRIDLPPADQAPISIPLVGAEIVDNAAAVVLRTYLVPVEGYCLYDPTIPLRLTNASV
                     TYEGTEAPPATVAEFLPPVLQRLTIFLPEQPSRAESDAAVRLTATTVAHYGRQPTNVT
                     VLPLPGSETAPPAPSQPLERQVVIRERPDSALTLQGTVGVPSLLITGPANDLTNQTRL
                     LSSDISRLALASKAVVGPLRSSPQLPPDLTTIRDLGQPGVNAVALSPQVSIGLDQTRL
                     GRSVRNVRVHLQGSYTPLPSSVAGEVVVSIGSDTVERWPTDNNGVIDRWVNVPDRLLQ
                     RYTNLGVAVNLAGNTGRCGEFQPVKLVIDGATVVTSQAAMPPAPPGFQSLPQAMLPRL
                     QVGIADDAFADTVRGAKIIEGLQRLSALPMETQVVSVADALNSELPAVVVSAQGWDDQ
                     RVQLPVSNRDNVIEVSGVGGDGPPETLTLDPSLQFGSLQTVFDGKRTVVVATSTAAPA
                     ELDALVGWLDADGRRWSRLSGDAVLQPAGRDPVTVASVAQPPAQPADRGRPWIWWVAG
                     ATAAVAVLGTGFAVLVNRRRKPGG"
     gene            complement(5751..6047)
                     /gene="rpsQ"
                     /locus_tag="VF874_14375"
     CDS             complement(5751..6047)
                     /gene="rpsQ"
                     /locus_tag="VF874_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX9177204.1"
                     /translation="MAETKGPKHTPRTEKPRGRRKTAIGYVVSDKMQKTIVVELEDRV
                     KHPLYGKIIRTTKKVKAHDENGDAGIGDRVSLMETRPLSATKRWRLVEVLERAK"
     gene            complement(6047..6280)
                     /gene="rpmC"
                     /locus_tag="VF874_14380"
     CDS             complement(6047..6280)
                     /gene="rpmC"
                     /locus_tag="VF874_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX9177205.1"
                     /translation="MAVGVSAGELRELTDEELIERLRESKEELFNLRFQMATGQLPNN
                     RRLRTVRHEIARVYTILRERELGLASGPDSEES"
     gene            complement(6280..6696)
                     /gene="rplP"
                     /locus_tag="VF874_14385"
     CDS             complement(6280..6696)
                     /gene="rplP"
                     /locus_tag="VF874_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX9177206.1"
                     /translation="MLIPRKVKHRKQHHPRQRGTASGGTTVSFGDYGIQALEHAYITN
                     RQIESARIAINRHIKRGGKVWINIFPDRPLTKKPAETRMGSGKGSPEWWVANVKPGRI
                     LFELSYPNEETARAALTRAIHKLPIKARIVTREEQF"
     gene            complement(6700..7536)
                     /gene="rpsC"
                     /locus_tag="VF874_14390"
     CDS             complement(6700..7536)
                     /gene="rpsC"
                     /locus_tag="VF874_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX9177207.1"
                     /translation="MGQKINPHGFRLGITTDWKSRWYADKQYADYIKEDVAIRRLLAT
                     GLERAGIADVEIERTRDRVRVDIHTARPGIVIGRRGTEADRIRADLEKLTGKQVQLNI
                     LEVKNPESQAQLVAQGVAEQLSNRVAFRRAMRKAIQSAMRQPNVKGIRVQCSGRLGGA
                     EMSRSEFYREGRVPLHTLRADIDYGLYEAKTTFGRIGVKVWIYKGDIVGGKRELAAAA
                     PAGAERPRRERPSGTRPRRSGASGTTATGTDVSRAAEEAPAVETTTPAEVPAAEAEST
                     ES"
     gene            complement(7536..8078)
                     /gene="rplV"
                     /locus_tag="VF874_14395"
     CDS             complement(7536..8078)
                     /gene="rplV"
                     /locus_tag="VF874_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX9177208.1"
                     /translation="MTTATEFPSATAKARFVRVSASKARRVIDLVRGKPVAEALDILR
                     WAPQQASEPVAKAIASAAANAQNNDGLDPSTLVVAALTADEGPTAKRIRPRAQGRAFR
                     IRRRTSHITVVVESRPRRDERTGQSTRAKRAAASKAATKKAPAKKAEPAKKAAPEAKK
                     AAGAAKPATSTSEEAKGGSQ"
     gene            complement(8075..8356)
                     /gene="rpsS"
                     /locus_tag="VF874_14400"
     CDS             complement(8075..8356)
                     /gene="rpsS"
                     /locus_tag="VF874_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX9177209.1"
                     /translation="MPRSLKKGPFVDDHLLKKVDVQNEKNTKQVIKTWSRRSTIIPDF
                     IGHTFAVHDGRKHVPVFVTEAMVGHKLGEFAPTRTFKGHIKDDRKSKRR"
     gene            complement(8366..9208)
                     /gene="rplB"
                     /locus_tag="VF874_14405"
     CDS             complement(8366..9208)
                     /gene="rplB"
                     /locus_tag="VF874_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX9177210.1"
                     /translation="MAIRKYKPTTPGRRGASVSDFAEITRSTPEKSLVRPLHGRGGRN
                     AHGRITTRHKGGGHKRAYRIIDFRRHDKDGVNAKVAHIEYDPNRTARIALLHYLDGEK
                     RYIIAPQGLRQGDVVESGPNADIKPGNNLPMRNIPAGTLIHAVELRPGGGAKLARSAG
                     SSIQLLGKEGSYASLRMPSGEIRRVDVRCRATVGEVGNAEQANINWGKAGRMRWKGKR
                     PTVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGQPEGRTRKPNKASNKLIVRRRRTGK
                     KHHR"
     gene            complement(9240..9542)
                     /gene="rplW"
                     /locus_tag="VF874_14410"
     CDS             complement(9240..9542)
                     /gene="rplW"
                     /locus_tag="VF874_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX9177211.1"
                     /translation="MATVTDPRDIILSPVISEKSYGLIEDNVYTFVVHPDSNKTQIKI
                     AIEKIFNVKVASVNTANRQGKRKRTRTGFGKRKDTKRAIVTLAPGSKPIDLFGAPA"
     gene            complement(9542..10186)
                     /gene="rplD"
                     /locus_tag="VF874_14415"
     CDS             complement(9542..10186)
                     /gene="rplD"
                     /locus_tag="VF874_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX9177212.1"
                     /translation="MKIDVLAPGGKKDGTVELPAELFDAPANIALMHQVVTAQRAAAR
                     QGTHSTKTRGEVRGGGRKPYRQKGTGRARQGSTRAPQFTGGGVVHGPKPRDYSQRTPK
                     KMVAAALRGALSDRARNGRIHAVTEIVSGQTPSTKSAGAFLGELTDRKRVLVVVGRTD
                     ETGAKSVRNLPGVHVLSPDQLNTYDVLLSDDVVFSVEALNAFIAANKQDEEVSA"
     gene            complement(10222..10875)
                     /gene="rplC"
                     /locus_tag="VF874_14420"
     CDS             complement(10222..10875)
                     /gene="rplC"
                     /locus_tag="VF874_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX9177213.1"
                     /translation="MARRGILGTKLGMTQVFDENNRVVPVTVVKAGPNVVTRIRTPER
                     DGYSAVQLAYGEISPRKVNKPLGGQYAAAGVNPRRHLAELRLDDPDAAAQYEVGQEVT
                     AEIFSDGSYVDVTGTSKGKGTAGTMKRHGFRGQGASHGAQAVHRRPGSIGGCATPARV
                     FKGTRMAGRMGNERVTTQNLMVHKVDAENGVLLIKGAIPGRTGGLVMVRSAIKRSEK"
     gene            complement(10890..>11036)
                     /gene="rpsJ"
                     /locus_tag="VF874_14425"
     CDS             complement(10890..>11036)
                     /gene="rpsJ"
                     /locus_tag="VF874_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX9177214.1"
                     /translation="SPHKYKDSREHFEMRTHKRLIDILDPTPKTVDALMRIDLPASVD
                     VNIQ"
BASE COUNT         1756 a         3749 c         3569 g         1962 t
ORIGIN      
        1 ccaactcgct accgcccaac cgacgaaggc ggcgaagccc ggtccgctga ccagcagcat
       61 gtccagcagt gcccgacgga gccgcggcgg cgggcgggcc cgtaggtacg tggtggcccc
      121 gaccaggatg cccgccgctg cgatgcaagc gaccgcgtcg taacgcgtca ggtacgcaag
      181 gcccatcgcg attccgcccg cggcaacgag gtgatgcacg tcgtcgtcga ccatccacat
      241 caccagccgg cgcaccgccc acgcgaggaa gaagatgaac ggggcctcac tcatgccgtt
      301 cgacccgtag aagacgatca tcgggttcaa cgcgaacagc atcacgatgc tcagcgaata
      361 ccatcggggc aggcctcggt cggtccccac gccgaggatt tgaatcaccg cgcccgccat
      421 gaagattgac gacatgatcg tgcccgagaa cgcacgttcg gtcatggccg gccacagcgg
      481 gctcatcgcg atccagggaa gctgcaccat cgcggtcagc ggggtgaaga tgaagccgat
      541 tgcggccaag tgcgggtcac ggctgaacag cacgctttgc gccgccgaca ctcgtgacag
      601 cgcgtcaccg aggatgaagc cgtggtccac ctgcaaccgg tagccgacgg ccagataccc
      661 caccacggcc accgcgaaca ccacgatctt gagtcgccgg tctgacatgg agccgacgat
      721 cacgttgcgg ttttctggtt ttcgggtcgg tccagcccgt gaaaggtctt ctcccagtaa
      781 gacgggttgc ggatcaactg atacaggccc ttcgccgccg caatgctcat catcaaccag
      841 aacagcggca cggtcagcgc cgggatcagc aggtcggatc ggtcgtcttc gcgcagcgca
      901 atcatgttca tgtacaacgt cgccgcattg ccgaggatca gcgcagcgag ggcgggaaag
      961 tagatgtacc aggggaacac cgcaccgatg gcggctggct ggcctaagaa ccacagcaga
     1021 gtgatcaacc agaacgcgag gttgagcacc gcaatgatcg gggttccccc caccaccagg
     1081 ttgaaccgga tgaagctgcg ccaaccgatg gtgcgcaaca gccggccggg tcgacgcatg
     1141 tgcacgagcc aggtctgcag ataacccttg taccaacggg atcgctgccg gatccagttg
     1201 atcgggtcgc tgttggcctc ctccatcgtg accgagtcga cgaccgcggt ctgatagccg
     1261 gtcgcggcga tgcgcaatcc caggtcggca tcctcggtga cgttgaacgg atcccatgcc
     1321 ccgatcttgt ccagcaccgg atgtagcaga tggtttgacg taccgcccag cgggataggg
     1381 gaattggtgc gcatcaggcc gggcagcaga tacccgaacc acagcccgta ctccgcggtg
     1441 aaccagccgg tgagcagatt ctgatggccg ttgtgatagg ccagtttcgc ttggatgcag
     1501 gccaccgagt cgggcagcgc atgaaacgcc gcgacgacgc ggcgcaactg cagtggctcg
     1561 ggtttgtcct ccgcgtcgta aatcgtgatg atctcgcctg tcgcgaactg cagaccgtag
     1621 ttgcacgcct tgggtttcgt gcgcggatcg gcaggcggca ccagcacaat cgtgatcgcg
     1681 tcggattcct ggcaccgctg cgcggccgtg atggtcacgt cgtcgtcggc ctccaacagc
     1741 aacaacacct gtagcttctc gcgcggatac tccagggcgg ccatggcccc gatgaggtcg
     1801 gccaccacct ccggctcgtt gtacgccggc accagaaccg tgtagggcgg caagtcggca
     1861 tcgggaatcc cccgcgcctc ttcgtcggtg acgacgatcg gccgcgccgc caacccgcgc
     1921 cggaagatca ggacgcgatc gatcatcgtg cagacgtagc cgaacgtgca gaggccgacc
     1981 aacgttacgg cggttcccat cggccgcagg atcgccagcg cgaccgtcgc caccaggatc
     2041 gtggcgagaa ctggtcgctg ccaacccatc accgccttcg cggcggagtg aatcgggtcg
     2101 tcttcacgca ggccctcgat cgccgcacgc aatgcctgtt cccgctcggc gtcggtgacg
     2161 tcgtgcaggt catcgctgcg gaacgctgcc gtcatggccc gaagcctcgc atcctggccg
     2221 ccacgagcgc ccggctgaac tcggtcagca tctcggcgtc cttgaacacc ggattgcggt
     2281 cgccgaccgc ggcattaccc cggaacagaa tggtgaacat cgagttcagc gtgccgggta
     2341 tgcctcccgc cggtatcggg aacggcgcat cgtcctcgtg gttgtccacg gacagcacga
     2401 cgatccgctg ggcttggcgc gcgttacgcc acgtccaaga cagcgcgttc caggtgacca
     2461 ccagcttgtc gtcgaccacc gacacgagct ggccggtgac tccatgaccg aggtcgaccc
     2521 ggcgcggcgc gctgagccgg gtagccgaca ggtcgtagag caaggtggtc gggtagacaa
     2581 cgaacgacat cgggcgcccg gtcaccacac tgtccaccac gacggtccgg gggtcggagt
     2641 gcttgtccca cttgggattc cccgagaggg ctatcatccg ctgacgcgtc acctcggcgt
     2701 cacgtccata gagcctgtgc acccacggga aggtctgctc gtcgatgatc ttccacccgg
     2761 ccggggcggg cagcggcgcg gcgaagttca tttcactgat ctgagttgtc ggcggggtcg
     2821 gcggggtggg cagtttcacc agcgagagca ggatcgcgaa cacggccacc agcgggaggc
     2881 ccacccacac ttgaccggcg gccaggggct cgatcttgcg gtccaggaaa ttcttttctt
     2941 tgccgcggcg cgcgtggagg tacagcaaga tcccgacgag caagatcgca gtcaacgccg
     3001 gaatctgttg ataggcaagc agcggcgcgt cgggaagata cagtgccatc acagcgagga
     3061 caccgaggcc cacagcccac gcgaggatcg agccgatgac cgcgcgccga accgaccggc
     3121 ccaccgcgat cgcggtggcg aaggcgccga cgatcaaggt cgccgcaccg cccgccatcc
     3181 ggttaccgcc gagcaagaag accgtgaggt ggtagggcag cgggaacacc atcagcagca
     3241 gcatccacac ccagaagaac cgagtgacag gccgaagccc gaacagcacg acggcggcgc
     3301 cgaccacgaa cagccacatc gccaggaagt cgagccgcag caggtggaag tacagcgcgt
     3361 aacgctgcat caggacggcg tgcaccagca gcgcgagtac caagcccagc gtcccgacga
     3421 tgatgtcggt ctggcggtca tggatcggca attcggtgcg cggccggcgg gccacgctgg
     3481 tgcccgccag cacagccgcc agcggcacaa tccacacgta gccgcccatg ccgcctccac
     3541 gggtcacctc cacgaggctc gccaggctgt gccggaacgc gatcaacgtg agcacgccga
     3601 tgtacagcca gcgcaacgca actcggtacc caggctgcag caggtgcagc catggctctc
     3661 gcctggcggt ggtgtccggc gctgccggct gcacggctgt cgtcacgtca gccgcccggt
     3721 ttgcgacgcc tgttcacaag gacggcgaac ccggtgccga gcaccgcaac cgccgccgtc
     3781 gcgcccgcca cccaccaaat ccacggcctg cctcggtcgg cgggttgagc gggaggctgt
     3841 gccaccgaag ctaccgtcac cgggtcgcgc ccggccggct gcaacacagc gtcgcccgac
     3901 agtcgagacc agcggcggcc gtcggcgtcc agccagccca ccagcgcgtc aagctctgcg
     3961 ggcgccgcag tcgacgtggc caccacgacg gtcctcttac cgtcaaaaac cgtttggaga
     4021 gagccgaatt gcagtgacgg gtcgagtgtc agcgtctccg gcgggccgtc accgccgacg
     4081 ccggagacct cgatcacgtt gtcgcggtta ctgaccggca actgaacgcg ctggtcgtcc
     4141 cacccttggg cggacacgac gacggcgggc agttcggagt tgagcgcatc ggccaccgag
     4201 accacctggg tctccatcgg caacgcgctg aggcgctgca gaccctcgat gatcttcgcg
     4261 ccgcgcaccg tgtcggcgaa ggcgtcgtcg gcgatcccga cctgcagtcg cggcagcatg
     4321 gcctgcggaa gcgactggaa gcccggcgga gcgggaggca tagccgcttg gctggtcacg
     4381 acggtggcgc cgtcgatgac cagcttgacg ggctggaact cgccgcatcg acccgtgttt
     4441 ccggccaggt tcaccgcgac gccgaggttg gtgtagcgct gcagcagacg atcgggaacg
     4501 ttcacccacc ggtcgatcac gccgttgttg tcggtcggcc accgctcgac cgtgtccgag
     4561 ccgatggaga cgacgacctc gcccgcgacg ctggacggca acggagtgta cgagccttgc
     4621 aggtggacac gcacgttgcg aaccgatcgt ccgagccggg tctggtcgag accgatggac
     4681 acctgtggtg acagcgccac agcgttgacg ccaggttggc cgaggtcacg gatcgtggtc
     4741 agatcagggg gaagctgtgg gctcgatctc agcggtccga caacggcttt cgatgccaac
     4801 gcgaggcgcg agatgtcgct cgagagcaac cttgtttgat tggtcaggtc gttggccggt
     4861 ccggtgatca gcaaggacgg cacacccacg gtgccctgca acgtcagtgc gctgtcgggt
     4921 ctttcgcgga tgacgacctg gcgctcgagc ggctgcgagg gcgcaggagg cgccgtctcg
     4981 gaaccgggaa gcggcagcac cgtgacattg gtgggctgcc tgccgtaatg cgccaccgtc
     5041 gtggcggtga ggcgcactgc cgcatcggat tccgctctgc tcggttgctc gggcaggaag
     5101 atcgtcaacc gctggagcac cggcggcagg aattcggcga cggttgccgg tggcgcttct
     5161 gtgccttcat aggttaccga cgcattggtg agccgcagcg ggatcgtggg gtcatacagg
     5221 caatagcctt ccaccggcac cagataggtg cgcagcacga cggccgccgc gttgtcgacg
     5281 atctcggcac cgaccagtgg aatgctgatc ggcgcctgat ctgcgggagg aaggtcaatc
     5341 cgcgacacca ccctgttgtc ctgcgtcacc gcgatcacgc cgctgcgcag attcggcggc
     5401 atttccacca caacgttgag tgcggtcggc gccagaccgc gcggcaccgg cactgtcagg
     5461 ttctgggtgc cctgcactcc gtagaacgcg aacgtcgagt cgacgcccag agactcgaga
     5521 ctcaaggtgg gcgactccgc cggaagcccc ccgtcgcccg gagcgctgtt cgccacggcg
     5581 ggaatccacc ccatcgtcag cacaagcgca atcacccacg ctcgaagaag ccaccccgtc
     5641 gccgccgcgt tcatcagcgc aaagggcagt caaccgggtc gcttagcacg ccttattgac
     5701 ggaattgtca tttttccgcg gccgcccggt tccttgctga gcagaccaga ctacttggcg
     5761 cgctcgagaa cctcgacgag ccgccaccgc ttcgtggccg acagcggccg cgtctccatc
     5821 agtgagacac ggtcgccaat gccggcgtcg ccgttctcgt cgtgcgcctt gaccttcttg
     5881 gtggtccgga tgatcttgcc gtacagcgga tgcttcaccc ggtcttcgag ctcgacgacg
     5941 atcgtcttct gcatcttgtc cgacaccaca tagccgatcg cggtcttacg acggccgcgc
     6001 ggcttctcgg tgcgcggagt gtgcttgggc cccttggttt ctgccattac gattcctcac
     6061 tgtcgggacc ggaggccagc cccagctcac gttcgcgcaa tatcgtgtag acgcgcgcga
     6121 tttcgtggcg caccgtacgc agccgacggt tgttcggcaa ctggccggtg gccatctgga
     6181 agcgaaggtt gaacagctct tccttcgact cccgcagccg ctcgatcagc tcctcgtcgg
     6241 tgagctcgcg cagttcgcca gcggacactc ccactgccat cagaactgct cctctcgggt
     6301 cacaatgcgt gccttgatcg gcaacttgtg gatcgcccgg gtcaatgcgg cccgcgcggt
     6361 ttcctcattg gggtagctca gctcgaacag gattcgtcca ggcttgacgt tggccaccca
     6421 ccactccggc gagcccttgc ccgaacccat ccgagtttcc gccggcttct tggtcaacgg
     6481 acggtcgggg aagatgttga tccacacctt gccgccacgc ttgatgtgcc ggttgatcgc
     6541 gatacgcgcc gactcgatct gccggttggt gatgtacgcg tgctccagcg cctggatgcc
     6601 gtagtcgccg aagctgaccg tggtgccgcc gctggcggtg ccgcgctgcc tcgggtgatg
     6661 ttgcttgcgg tgcttgacct tacggggaat caacatggct cagctctccg tgctctcggc
     6721 ctcggcggca ggcacctcgg ccggcgtcgt cgtctcgacg gccggagcct cctcggccgc
     6781 ccggctcacg tcggtgcccg tcgcggtggt gcccgatgcg ccgctgcggc ggggccgggt
     6841 acccgacgga cgctcgcggc gcggacgctc tgcgcccgcc ggtgcggccg cggccagctc
     6901 gcgcttgccg ccgacgatgt cgcccttgta gatccacacc ttcacgccga tccggccgaa
     6961 cgtcgtcttc gcctcgtaaa ggccgtagtc gatatccgcg cgcaacgtgt gcagcggaac
     7021 gcggccctcg cggtagaact ccgagcgact catctccgcc ccgccgaggc ggcccgagca
     7081 ctgcacgcgg attcccttga cgttgggttg acgcatcgca gactggatcg ccttgcgcat
     7141 cgcgcggcgg aacgccacgc ggttagacag ctgctcggcc acgccctgcg caaccaattg
     7201 cgcctgcgac tcagggtttt tcacctcgag gatgttgagc tgaacctgct tgccggtgag
     7261 cttctcgagg tcggcgcgaa tgcggtcggc ctcggtgccg cgccggccga tcacgatgcc
     7321 gggccgcgcg gtgtggatgt cgacgcgaac ccgatcgcgg gtgcgttcga tctccacgtc
     7381 ggcgatgccg gcgcgttcca gaccggtggc cagcagcctc cggatcgcta catcttcctt
     7441 gatgtagtcg gcgtactgct tgtcggcgta ccagcgggac ttccagtcgg tggtgatccc
     7501 gagccggaag ccgtgcgggt tgatcttctg gcccactact gcgagcctcc cttcgcttct
     7561 tctgaagtcg atgtcgccgg ctttgcggct ccggccgcct tctttgcctc tggcgcggcc
     7621 ttcttcgccg gttcagcctt cttggcgggc gccttcttcg tggccgcctt gctcgccgcg
     7681 gcccgcttcg cgcgcgtcga ctgacccgtc cgctcgtcgc gacgcggccg gctctccacc
     7741 acgaccgtga tatggcttgt ccgcctgcgg attctgaacg cgcggccctg cgcgcgcggc
     7801 cggatgcgct tggcggtcgg gccctcgtca gcggtcaacg ccgcgacgac gagcgtcgac
     7861 gggtcgagcc cgtcgttgtt ctgcgcgttg gctgcggcgc tggcgatcgc cttggccacc
     7921 ggctcgctcg cctgctgcgg cgcccaccgc aaaatgtcca acgcttcggc caccggcttg
     7981 ccgcgcacca ggtcgatcac cctgcgcgcc ttgctcgccg acacccgcac aaaccgcgcc
     8041 ttggcggtcg ccgaagggaa ttcggtggct gtcgtcatcg tctcttgctc tttcggtcgt
     8101 ccttgatgtg gcccttgaac gtccgagtcg gcgcgaactc accgagcttg tgcccgacca
     8161 tcgcctcggt gacgaacacc gggacatgct tgcgaccgtc gtgcaccgca aaggtgtgcc
     8221 cgatgaagtc ggggatgatc gtggaccgac gcgaccaggt cttgatcacc tgcttcgtgt
     8281 tcttctcgtt ctgcacgtcg accttcttga gcagatggtc gtcgacgaac ggacccttct
     8341 tcaagctgcg tggcatcgtc tactcctacc ggtggtgctt cttgccggtg cgccggcgtc
     8401 ggacgatgag cttgttgctc gctttgttcg gtttgcgggt gcggccctcg ggctggcccc
     8461 acgggctgac cgggtgacgg ccgccggagg tcttgccttc gccaccgccg tgcgggtggt
     8521 cgaccgggtt catcacgaca ccacgcacgg tgggccgctt gcccttccag cgcatgcgac
     8581 cggccttgcc ccagttgatg ttcgcctgct cggcgttgcc cacttcaccg accgtggcac
     8641 ggcagcgcac gtccacgcga cggatttcgc cgctgggcat gcgcaacgac gcgtagctgc
     8701 cttccttacc gagcagctgg atgctcgatc cggccgaccg agccagcttc gctccgccgc
     8761 cggggcgcaa ctcgaccgcg tggatcaacg tgccggcagg gatgttgcgc atcggcaggt
     8821 tgtttcccgg cttgatgtcg gcgttcgggc ccgactcgac gacgtcgccc tgacgcaggc
     8881 cctggggcgc gatgatgtag cgcttctcgc cgtccagata gtgcagcagc gcgatacgcg
     8941 cggtgcgatt cgggtcgtac tcgatatgag cgaccttcgc attcacgccg tccttgtcgt
     9001 gccggcggaa gtcgatgatc cggtaggcgc gcttgtgccc gccacccttg tgacgagtcg
     9061 tgatgcggcc gtgcgcgttt cgtccgccac ggccgtgcag cggacgcacc agcgacttct
     9121 ccggggtcga ccgggtgatc tcagcgaaat ccgagacgct ggcaccgcga cggccgggcg
     9181 tcgtgggctt gtacttgcga attgccatgt ctgctatctc ttcttgtctt gccgcgcggc
     9241 tacgccggcg ctccgaacag gtcgatcggc ttgctgccgg gggccagggt cacgattgcg
     9301 cgcttggtgt ccttgcgctt gccgaaaccg gtgcgggtgc gcttgcgctt gccctgccgg
     9361 ttcgcggtat tcaccgacgc cactttgacg ttgaagatct tctcgattgc gatcttgatc
     9421 tgcgtcttgt tggagtcggg gtgcacgacg aacgtgtaca cgttgtcctc gatcagcccg
     9481 taggacttct ccgagatgac cggggacaag atgatgtcgc gggggtcggt gacggtcgcc
     9541 atcaggccga aacctcctcg tcctgcttgt ttgcggcgat gaacgcgttc aaggcctcga
     9601 cggaaaacac cacgtcgtcg gacagcaaca cgtcgtaggt gttcagctgg tcgggtgaaa
     9661 gcacatgcac accgggtaaa ttgcgcacgc tcttggcgcc cgtctcgtcg gtgcggccga
     9721 cgaccaccag cactcgcttg cggtcggtca gctcgccgag aaacgccccc gcgctcttcg
     9781 tcgacggcgt ctgacccgac acgatctcgg tcaccgcgtg aatccggccg ttgcgcgcac
     9841 ggtcggacag cgccccgcgc agcgcggcgg cgaccatctt cttcggcgtc cgctggctgt
     9901 agtcgcgcgg cttggggccg tgcacgacgc caccgccggt gaactgcggc gctcgcgtcg
     9961 acccctggcg agcgcgcccc gtgcccttct ggcggtacgg cttgcggccg ccgccgcgga
    10021 cctcgccgcg cgtcttggtg gaatgcgtgc cctggcgggc ggccgcacgc tgggccgtca
    10081 ccacctgatg catcagggcg atgttggccg gcgcgtcgaa cagctcggcg ggcagttcca
    10141 ccgtgccgtc cttcttgccg ccgggcgcga ggacgtcaat cttcagagcc gctccgcccg
    10201 tcttgctcct tgcgtccgca gtcacttctc actcctcttg atcgcgctgc ggaccatgac
    10261 cagtccccct gtgcgtccgg ggatggcgcc cttgatcagc agcacaccgt tctcggcatc
    10321 gaccttgtgc accatcaggt tctgcgtcgt gacgcgctcg ttgcccatcc gaccggccat
    10381 gcgggtgccc ttgaaaaccc tggcaggagt tgcacatccg ccgatcgagc ccggccgccg
    10441 gtgtaccgcc tgcgcgccgt ggctggcgcc ctggccccgg aacccgtggc gcttcatcgt
    10501 gccggcggtg cccttgccct tcgaggttcc ggtcacgtcg acgtagctgc cgtcactgaa
    10561 gatctcggcg gtcacctctt ggcccacttc gtactgcgcc gccgcgtcgg gatcgtcgag
    10621 ccgcaactcg gccagatggc ggcgcgggtt cacgcccgcc gccgcgtact ggccgcccaa
    10681 cggcttgttc accttgcgcg ggctgatttc gccgtaggcc agctgcacag cgctgtagcc
    10741 gtcgcgctcc ggggtgcgga tgcgcgtgac gacgttcggt cccgccttga ccaccgtcac
    10801 cggcacgacg cggttgttct cgtcgaacac ctgcgtcatg cccagcttgg tgcccagaat
    10861 ccctcttctc gccatgaatg tctggtctcc tactggatgt tcacgtcgac gctggccggc
    10921 aggtcgatgc gcatcagcgc gtccaccgtt ttcggcgtcg ggtcgaggat gtcgatcagc
    10981 cgcttgtggg tgcgcatctc gaagtgctcc cgcgagtcct tgtacttgtg cgggga
//
LOCUS       DASWBF010000386         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_297560,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000386 DASWBF010000000
VERSION     DASWBF010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297560"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VF874_14430"
     CDS             <1..751
                     /locus_tag="VF874_14430"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9177215.1"
                     /translation="PDRFGYFATLPLPDVAAAVDEAVRALDEDGASGVLMLSNHAGLY
                     QGDTMLDPLYEELNSRRAVVFVHPAEPGAGNPLPQIQTSQLEFGFDATRAVANLLING
                     VPQRFPAIRFVLTHSGSCVPSVAHKLINRKPMVAAYTAYVREHGTPPPVDELLDQLSA
                     AENAARQQIAGLYFDTALSTAARVLDALAALVPTSHILLGTDFPFGQEIGLRYTLRSI
                     ERYRGFDDDDRAAILRTNARDLLAHGTPATP"
     gene            complement(788..1312)
                     /locus_tag="VF874_14435"
     CDS             complement(788..1312)
                     /locus_tag="VF874_14435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9177216.1"
                     /translation="MNCTHPHVVVTGASTGIGRATAVRLAEAGWHVFAGVRRHVDGDS
                     LREAAGESGARVTPVLMDVTATDQIDAGVVTVTDHVGPSGLDGLVDNAGIGVTSPMEL
                     VPLDALRLQFEVNVIGQVAVTQAFLPLLRQAKGRIVVIGSIGDRFTPPTKRSTNPSLC
                     MSIWRCAPVRAVSR"
     gene            complement(1309..1566)
                     /locus_tag="VF874_14440"
     CDS             complement(1309..1566)
                     /locus_tag="VF874_14440"
                     /inference="COORDINATES: protein motif:HMM:NF044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177217.1"
                     /translation="MTSQFPMVPDYVVLPATSPRVDLAATFFVDVADTLRRKYRIEKE
                     AMLLIRPDGYLALREDRWAPTELRSHLRRWLIPSVSILEES"
     gene            complement(1606..>2755)
                     /locus_tag="VF874_14445"
     CDS             complement(1606..>2755)
                     /locus_tag="VF874_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9177218.1"
                     /translation="ERLGVIDQTVARGLPVRKFNILSEEHRIAEFDLGRLDTSYPYLL
                     MLPQNLTEEILRDRLEELGGCVEHGVELVSFTQDADGVDAVLRGDGRTERVGADWLIA
                     ADGAHSTFRHQLGLQFEGGKFREEFTVADIRVDWTLPMDELFAYLNRGNFIAYFPMAG
                     GWHRVAIAYNTRPAPVGDVTLAEVQDAVDIRGPRGARVVKIGDRSRFVINQRTVEKQS
                     VGRIFLAGDAAHVNSVMGAQGLNIGVQDSFNLAWKLAAVINGSSSASLLDTYAAERRP
                     AARRIVKGTTRATRLTLLRRPLAVMARRHLAPLVLRRARVRTAVEHALSQLDISYRQP
                     GAGAGRPATGDRALDAPLRQYQNGRPVDPVRRLFDVIREDEFSLLLVG"
BASE COUNT          494 a          934 c          881 g          446 t
ORIGIN      
        1 gccggatcgg ttcgggtact tcgcgaccct gccactgccc gacgtggcgg ccgccgtcga
       61 cgaggcagtg cgcgcactcg acgaggacgg cgcgtcgggg gtgctgatgt tgagcaacca
      121 cgccggcctc taccagggcg acaccatgct cgacccgctt tacgaagagc tcaacagcag
      181 gcgcgcggtg gtgttcgtcc atccggccga accgggcgcc ggcaacccgc tgccgcagat
      241 tcagacctca cagctggaat tcggcttcga cgcgacccgc gcagtggcca acctcctgat
      301 caacggagtg ccgcaacggt ttccggcgat ccggttcgtg ctcacccaca gcggcagctg
      361 cgtgccgtcc gtggcgcaca agctgatcaa ccgcaaaccc atggtcgccg cctacaccgc
      421 gtacgtccgc gagcacggaa cgccacctcc ggtcgatgag ctcctcgatc agctcagcgc
      481 tgcggaaaat gcggcgcgac aacagatcgc cgggctgtac ttcgacacgg cgctgtcgac
      541 ggccgcgcgg gtgctggacg cgttggcggc gctggtgccg acgtcacaca tcctgctggg
      601 caccgacttt cctttcggcc aggagatcgg actgcgctac acgctccgca gtatcgaacg
      661 ctaccgcgga ttcgacgacg acgatcgtgc cgcgattctg cggacgaacg ctagagatct
      721 gctagcgcac ggtacgcccg ccacacctta gcgatcccgt actcggccac cagcaatagc
      781 accgcgttca cctgctcacg gcccgcaccg gcgcgcatcg ccaaatcgac atgcagagcg
      841 aaggattcgt cgagcgtttg gttggcgggg tgaagcgatc gccgatggag ccgatcacca
      901 cgatgcggcc cttggcctgc cgcagcagcg gcagaaacgc ctgggtgacc gcgacctggc
      961 cgatcacgtt gacctcgaac tgaagacgca gcgcgtccag cggtaccagt tccatcggag
     1021 acgtcacacc gataccggcg ttgtcgacca agccgtccag accagacggg ccgacgtggt
     1081 cggtgacggt cacgacgccc gcgtcgatct gatcggtggc ggtgacgtcc atcagcacgg
     1141 gcgtcacgcg ggcaccggat tctcccgccg cctctcgcaa cgagtcgccg tcaacgtgcc
     1201 ggcgcactcc cgcgaagacg tgccagccgg cctcggcgag acgcaccgcc gtggcccgcc
     1261 cgatgccggt cgaggcaccg gtgaccacca cgtgtggatg tgtgcaattc atgattcctc
     1321 caggatggat acggatggga tcaaccagcg ccgcaggtgc gatcggagtt cagttggcgc
     1381 ccaacggtct tcgcgtagcg caaggtaccc gtcgggtcgg atgagcagca tggcttcctt
     1441 ctcgatgcgg tacttgcgtc gcaacgtgtc agcgacgtcg acgaaaaacg tcgcagcaag
     1501 atctacacgc ggcgatgtgg cgggcagcac gacgtagtcg ggaaccatgg ggaattggga
     1561 tgtcacacac cgaatctgct cgtagcgtgc cgccaaatcc gctgcctacc ccaccagtaa
     1621 cagggagaac tcatcctctc ggatcacatc gaagagcctc ctgacaggat cgactggtcg
     1681 gccgttctgg tactgccgca gcggcgcatc gagggcgcgg tcgccggtcg ccggtcggcc
     1741 agcacccgcg cctggctggc ggtaggagat gtccagttga gacaacgcat gctcgacggc
     1801 ggtccgaacc cgtgcccggc gtaataccag cggtgcgaga tggcggcggg ccatcacggc
     1861 aagcggacgg cgcagcagcg tcaaccgggt cgcgcgcgta gtgcccttga caatgcgcct
     1921 agcggcgggc cgtcgctcgg cggcgtaggt gtcgagcagg ctcgcagacg agctgccgtt
     1981 gatcaccgcg gccagtttcc acgccaggtt gaacgagtcc tgcaccccga tgttgaggcc
     2041 ctgcgcgccc attaccgagt tgacgtgcgc ggcatcaccg gccaggaata tccgcccaac
     2101 ggactgcttt tcgacggttc tctggttgat gacgaagcgt gacctgtcac cgatcttcac
     2161 tacacgggca ccgcgcggac cgcggatgtc gacggcgtcc tgcacctcgg ccagcgtcac
     2221 gtcgccgacc ggcgcaggcc tggtgttgta ggcgatggcc acccggtgcc aaccaccggc
     2281 catcgggaag tacgcgatga agttgccgcg gttgaggtag gcgaacagct cgtccatcgg
     2341 cagtgtccag tcgacccgga tgtcggcgac cgtgaactcc tcgcggaact tcccaccctc
     2401 gaactgcaac cccagctggt gacggaacgt actgtgcgca ccgtcggcgg cgatcagcca
     2461 atcggctccg acccgttcgg tgcgcccgtc gccgcgcaag accgcgtcga cgccgtcggc
     2521 gtcctgcgtg aagctgacga gctccacccc gtgctcgacg cacccgccga gttcctccaa
     2581 tcgatcacgc aggatctcct cagtcaggtt ctgcggcagc atcagtagat acgggtacga
     2641 cgtgtccagc cttccgaggt cgaattcggc gatgcgatgt tcctcggaga ggatgttgaa
     2701 cttgcgcacc ggcaggcctc gggcgacggt ctggtcgatc accccaaggc gctcg
//
LOCUS       DASWBF010000387         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_44515, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000387 DASWBF010000000
VERSION     DASWBF010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44515"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VF874_14450"
     CDS             complement(<1..523)
                     /locus_tag="VF874_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177219.1"
                     /translation="MTALELLAPAQTPSVPSATRADQLAALLTPTFVEAVGWNPEHVS
                     LWLEPGPMFRNHRCPSLGCHRIAPGSDGQVCATCQRQARVRGVSIEEIIRIGPESNGP
                     SRGIPTAPCDVAGCGRIRARRRLCVVHDHRRLARKLSQAAYIASNPEPLDSFGECAVP
                     ACLRDALHRNSFCI"
     gene            complement(520..1719)
                     /locus_tag="VF874_14455"
     CDS             complement(520..1719)
                     /locus_tag="VF874_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9177220.1"
                     /translation="MDPAWSAFKLDTRPGAAPYRRFLVQLPTGDRYYTVLGADYLRVK
                     HCDEFLFQHLARGGAEGTTESYAGAIALFLTWCSATDRSVATAARFLGRFMFWLRRYD
                     TTADAMVGPGLPDVRGDSRINTVLAAVREFYKHLVATRAVPASTLDALYRIVDDRDLP
                     DDVRGESATLRYRMSPHHRIPLGDKGTKRATEAETIALLRACRSARDRLIVVLAVRAG
                     MRRGEIAGLRLEDLHLMHGQRAGCRHDGPHVHVVKRLNPNRAAAKSRHARELPCDPMV
                     VRLFDDWFAERDTIPGAAACDFVLITLTGPHTGSGPRPGLINEIFKALVRRAGLDRNV
                     HPHMLRHTMLSNVIDHGGTLDEAQALAGHASPATTAVYTHPAASRLRAAVERVPSPRI
                     DTWKATQ"
BASE COUNT          341 a          595 c          651 g          315 t
ORIGIN      
        1 cgatgcaaaa cgagttgcgg tgcaacgcgt ctcgcaggca ggcgggaacg gcgcactcgc
       61 cgaaggagtc gagtggttcg gggttggacg cgatgtaggc cgcttggctc agtttccgag
      121 cgaggcggcg atggtcgtga acgacgcaca gtcgtcggcg cgcacgaatc cggccgcaac
      181 cggcgacatc gcagggcgct gtgggtatgc ctctgctggg gccgttcgat tccggtccga
      241 tacggatgat ctcctcgatc gacacgcctc gaacgcgtgc ttgacgctgg caggtcgcgc
      301 atacctggcc gtcgctaccg ggcgcgatgc gatggcatcc cagcgatggg catcggtggt
      361 tgcggaacat cgggccgggt tcaagccaca gcgagacatg ctcgggattc cagccgacgg
      421 cttcgacgaa cgtcggcgtc agcaacgctg cgagctggtc agcgcgggtg gcggacggca
      481 cggacggtgt ctgcgccggc gccaacaact ccaatgcggt cactgcgtgg ccttccaggt
      541 gtcgatgcgt ggactgggga ctcgttcgac ggcggcccgc aagcgagagg cggcaggatg
      601 cgtgtaaaca gcggtggtgg cggggctggc gtgcccggcg agcgcttggg cttcgtcgag
      661 tgtgccgccg tgatcgatga cgttgctgag cattgtgtga cgaagcatgt gcgggtgaac
      721 gttgcggtcg agacccgctc gccggaccag ggctttgaag atctcgttga tcagtcctgg
      781 ccgcgggccc gaaccggtgt gtggcccggt aagagtaatg agcacgaagt cgcaggcggc
      841 cgcgccgggt atcgtgtcac gctcggcgaa ccaatcatcg aacagccgta ccaccattgg
      901 gtcgcaaggc aattcacggg cgtgccgcga tttggctgca gcgcggttgg gattgagtcg
      961 cttgacgacg tgcacgtgcg gaccgtcgtg acgacagcca gcacgctggc cgtgcatcag
     1021 gtgaagatct tccaggcgca gtcccgcgat ctcgccgcgg cgcattccag cgcgaacggc
     1081 caagacgacg atcagccggt cacgggcgct gcggcacgcg cgcagcagcg caatcgtctc
     1141 ggcttcagtt gcacgcttgg ttcccttgtc gccgagaggt attcgatggt gaggactcat
     1201 ccgatatcgc agcgtggcgc tttcgccgcg gacgtcgtca ggcaggtcac gatcgtcgac
     1261 gatcctgtac agcgcatcga gtgtgcttgc cggaaccgcc cgcgttgcga ccagatgttt
     1321 gtagaactcg cggacagcgg ccagaacggt gttgatccgc gaatcgccgc gcacatccgg
     1381 caggcccggc ccgaccatgg cgtccgccgt ggtgtcgtag cggcgaagcc agaacatgaa
     1441 ccggccgagg aatcgcgccg cggttgcgac gctgcggtcc gtcgccgagc accaggtcag
     1501 aaacagcgct atcgctccgg cgtaactctc ggtcgtgcct tccgcgccac cccgggcaag
     1561 gtgctggaac aggaactcgt cgcagtgctt cacccgcaga tagtcggcgc ccagcacggt
     1621 gtaatagcgg tcgccggtcg gaagctgcac caggaaccgc cggtacgggg cagcgccggg
     1681 tcgtgtatcg agtttgaacg ccgaccaagc cggatccatc ggcactcccc accgatctgc
     1741 gggacgctgt cgagcttcaa cgctaaccgc agcatcgcca ggacgaggtg cacgacacac
     1801 tccgacgtgt ccaacacgcc gacggaagat gtcgtacacg atgcgcccga actgtcaacc
     1861 acgacagtcc ccgacaccac tacctacccg acggaaccct cg
//
LOCUS       DASWBF010000388        23448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1225639,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000388 DASWBF010000000
VERSION     DASWBF010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23448
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225639"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VF874_14460"
     CDS             complement(<1..416)
                     /locus_tag="VF874_14460"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9177221.1"
                     /translation="MLGDLYEQALTGKRCWLRHDDGRVHPLPVRSWLGGRGADDVFDR
                     AVVGMCDGPTIDLACGPGRLVAHLIRRGIPALGVDQSATAVQLARRSGAPALHRDVFD
                     PLPGTGRWHTVLLADGNVGLGGDPRRILVRASELLRR"
     gene            complement(418..1080)
                     /locus_tag="VF874_14465"
     CDS             complement(418..1080)
                     /locus_tag="VF874_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HEX9177222.1"
                     /translation="MTVPVTVLVVAKAPVPGVAKTRLAAAVGPEVAADIAAAALLDTL
                     DAVAAAPVTARVVALTGDLDAASRSDEIRERLADYFVIPQRGMDFADRLANAHADTAA
                     VAGPHPVLQIGMDTPQVTAALLTRCCDALLASPAVLGMASDGGWWVLGLHSPTHADVL
                     RAVPMSQGDTGAVTLRALRDTGINVVLVDELRDVDTIEDVAAVRDVSPSGSRFARTTL
                     GV"
     gene            complement(1077..1733)
                     /locus_tag="VF874_14470"
     CDS             complement(1077..1733)
                     /locus_tag="VF874_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9177223.1"
                     /translation="MPNCSVTVVLPCLNEAASLPGVLAKIPPGYRALVVDNNSDDDTA
                     GLARRHGAQVVTEQRPGYGSAVHAGVVAATTPIVAVLDADGSLDPAELPLMVAELERG
                     ADMVTGRRRPVRGLHWPWHARLGTAAVSWRLRTRHRLSVHDIAPMRVARRDALLRLGV
                     EDRRSGYPVELLVRAAAAGWTVVERDVGYGPRTGGKSKVSGSARGSLVAAVDFWRAIS
                     "
     gene            1837..2526
                     /locus_tag="VF874_14475"
     CDS             1837..2526
                     /locus_tag="VF874_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9177224.1"
                     /translation="MNARVLIADDDEVVRDVVRRYLERDGLDVTVAGDGAEALRVLGA
                     EPIDVAVLDVMMPGPNGLTLCRTLRSGGGYPVPVILLTALSEEEDRIAGLEAGADDYV
                     TKPFSPRELALRVRSVLRRSSSGASALPVEVAAEGLKMLTASRSAYLDERKISLTNRE
                     FDLLLFFLTHTDTVFTREELLKLVWRWEFGDLSTVTVHVKRLRTKLGDRHRIQTVWGR
                     GYMWGEPHDGT"
     gene            2513..3535
                     /locus_tag="VF874_14480"
     CDS             2513..3535
                     /locus_tag="VF874_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9177225.1"
                     /translation="MTAHDLLRIAAWALGCTVPVVLFGWLIIRLARSWSLTVSMVVLV
                     LIPTLATLAGVLGASGFMFTHTFRYVAVILVIVAVVTIPAAVILARFQARRMVWENQI
                     RDAERASEESRRQLVAFVSHDLRTPMAGIRAVAEAMADGVVPEREVRQHAKHIEQESV
                     RLTEMVNDLFEMSKINAGAVELPQEKVALDEVVDDVLAAHKIAAEHAGVALQATLPAE
                     PVQVIGSDRALMRVLSNLVANAIAHTPSGGTVTLTLGADENGAFAQVDDTGVGIDEKD
                     LPKVFDVAYRGSNSRVHGSDPALPSGSGLGLAIAAGLVQAHHGTLSAHNLGTGARFEV
                     RLPLAD"
     gene            3576..4010
                     /locus_tag="VF874_14485"
     CDS             3576..4010
                     /locus_tag="VF874_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX9177226.1"
                     /translation="MAKTGLRKFKFERQVGRAVVNPLVAALDRRGIRSKLVVELETTG
                     RKSGEPRHVPLTGSADEGGVWVISQHGHRAGWAHNISADPRVRVRVNGRWRTGTAAFR
                     PDDDVRARARSFGSGKVSGTAAALAMRAMESDPISVRITYTG"
     gene            complement(4007..4798)
                     /locus_tag="VF874_14490"
     CDS             complement(4007..4798)
                     /locus_tag="VF874_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9177227.1"
                     /translation="MASTRKSPRWLPPRRLIGALQRRLIYFPSPGSVRSAATVLRGGQ
                     DVVLHTDDGLRLGAWFFPADGHSAVLMCNGNAGDRCMRAPLAAALVRNGLSVLLFDYR
                     GYAGNPGRPSEDGLAADARAARTWLDAHSDRIAYLGESLGAAVALRLAVEHPPSALML
                     RSPFTSLTDVGRLHYPRLPVAQLLSDRYPSIDRIENLGAPLLVIAGERDSIVPAELSR
                     RLYDAAPEPKRFVLVPGADHNDPELLDGPEVVAEIVRFAQASERP"
     gene            complement(4821..5855)
                     /locus_tag="VF874_14495"
     CDS             complement(4821..5855)
                     /locus_tag="VF874_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9177228.1"
                     /translation="MRVLLTGAAGFIGARVAAALAAGGHDVVGVDVMLPAAHGPGSAS
                     PEGCLRIDVRDADALAPLLSGVDVVCHQAAMVGAGVDAADAPEYGSHNDYATTVLLAQ
                     MYAAGIRRLVLASSMVVYGQGRYDCPVHGHVDPLPRRREDLDAGVFEHRCPLGGEELS
                     WRLVDEDAPLRPRSLYAASKTAQEHYGLAWSDAVDGSVIALRYHNVYGPGMPRDTPYS
                     GVAAIFRSALEKGEPPQVFEDGGQMRDFVHVDDVAAANVAAVNRVGSERFLAANVCSG
                     QPISIHEVAAQLSEASGGPSPQVTGYYRSGDVRHIVADAARAREILGFTARIRPEAGL
                     REFATAPLRN"
     gene            complement(5852..6625)
                     /locus_tag="VF874_14500"
     CDS             complement(5852..6625)
                     /locus_tag="VF874_14500"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX9177229.1"
                     /translation="MLGVIGGSGFYTFLDEGVRSVEVETPYGPPSGPITVGLVGDHEV
                     AFLPRHGVRHQFSPHAVPYRANMWALRALGVRRIFGPCAVGSLTRELGPGATVVPDQL
                     VDRTSGRDDTYFDSGGIHVAFADPYCPTLRQAALGPGIVDGGTMVVIQGPRFSTRAES
                     RWFASQGFTLINMTGYPESVLARELEMCYAAIALVTDLDAGIDVGSGVRAVDVFAEFE
                     RNLGPFKKVVLDALRAVESERTCTHCLAHEGVQLPFELP"
     gene            6660..7532
                     /locus_tag="VF874_14505"
     CDS             6660..7532
                     /locus_tag="VF874_14505"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX9177230.1"
                     /translation="MASVTQWIEGARPRTLPNAVAPVIAGTGAAAWLHAAVWWKALLA
                     LVVAVAMIVGVNFANDYSDGIRGTDDERAGPLRLVGSRLATPRSVLAAAAVSLGVAAA
                     GGVVLAVASAPWLIAVGAVCIAGAWLYTGGSRPYGYRGLGEVAVFVFFGLVAVLGTQY
                     VQALRVDWVGAVLAVATGSLSSAVLVANNLRDIPTDARSGKVTLAVRLGDARTRLLYQ
                     GLLGVAALGTLALIAATPWAAIGFVAMPLALRAAAPVRRGQGGRELIPVLRDTGLTML
                     VWSVAVALALALAA"
     gene            complement(7624..7959)
                     /locus_tag="VF874_14510"
     CDS             complement(7624..7959)
                     /locus_tag="VF874_14510"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9177231.1"
                     /translation="MIHRFTKVIATAIVAGGFGLAAVATATTASALTGDNAQFFDDIE
                     AAGIGYDSPEAAVKNAQLVCQLLSEGNSPNSIGREIVGNSDLSSGQATAFVTASVDSF
                     CPKYAGALN"
     gene            complement(8131..10005)
                     /locus_tag="VF874_14515"
     CDS             complement(8131..10005)
                     /locus_tag="VF874_14515"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9177232.1"
                     /translation="MSDTDSRVGTDFGHYHLRRLLGKGGMGEVYEAEDTRKDRTVALK
                     VLPATMSNDPVFRKRLQREAHSAGRLQEPHVVPIHDYGEIDGVLYVDMRFIDGTDLRK
                     MLVRYGPLTPARAVAIIRQVASALDAAHESGLVHRDVKPENILITRDDFAYLVDFGIA
                     NAKTDEKLTELGTAVGTYAYMAPERFTNGEVTYRADIYALACVLHECLTGAQPYPADS
                     VSVVITAHMMQPIPKPSAARPGIPAAFDEVVSRGMAKDPKDRYATAGDLATAAHEALA
                     SPDQDKADTILARSEAATMPGPLAPSATPASFATPPPSFSTPPPRATPPPPSFTTPPP
                     SFTTPPPGSSTPPGSSTPPPTSPPYVAASSQPPYQPPYQPPGTGGTGGWGPGGPTGPG
                     GPPSGGPPWQPPPSGPMPSAQPPKRNWGPIIAAIVAFVVVVILAVLAVVLIPKFTKSD
                     ENTTTTTTTTSAPTTTTHTKTRTTTTRTTPPTTGQSGASARLMSILPDGYEPGACKPI
                     DPVTDALASVDCEKNTKPGGPEVARYSLFPDQATLDRHFETAIKEDDQLQNCPGSDTP
                     SPTTWHYTSTPDKVEGQVACGTYKGGPDMLWTRNADLMLADGQGPDMVALHDWWLKFG
                     "
     gene            complement(10223..11824)
                     /locus_tag="VF874_14520"
     CDS             complement(10223..11824)
                     /locus_tag="VF874_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9177233.1"
                     /translation="MSDSESRAGTDFGHYHLRRVLGKGGMGEVYEAEDTRKDRTVALK
                     LLPPAMSHDPVFRKRLEREARSAGRLQEPHVVPIHDYGEIDGVLYVDMRFIDGTDLRK
                     VLVRYGPLTPARAVAIIRQIASALDAAHESGLVHRDVKPENILITWDDFAYLVDFGIA
                     NATTDEKLTELGTAVGTYAYMAPERFSNSEVTYRADIYALACVLHECLTGVQPYPADS
                     VSVLITAHLMQPIPHPSAVRPGVPQAFDAVISRGMAKDPKDRYATAGDLAAAAHDALA
                     SADRDTSDNILSRSAAATLPGLITPPPGITTPPPGSVTPPPARFMTPPPGSSTPPPST
                     PPPYVAASSQPPYQPPGTTASSGPGGPGGPPPWQTSASGPMPSAPPPKRRNYLPIIAA
                     LAAFVVVLIGVVTAVLLMSGSDDKNNNAATDTTTPSEDTTTTRTRTTTTRTTATSQPP
                     VNTSGMWVVIVFSPATGAFGWSNNATSQDQATNTATDYCKQYGGTDCQVAAYATDGCA
                     ALAQSDTTWHGGLGATIEDAEKDAL"
     gene            complement(11889..13082)
                     /locus_tag="VF874_14525"
     CDS             complement(11889..13082)
                     /locus_tag="VF874_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX9177234.1"
                     /translation="MTQTGLGFGVLGPLQMTVDGTPVSLGTPKQRAVLAMLVINRNRP
                     VSIDALITAAWEQWPPAEARASLHSYVSNLRRLLAKSGVDPRAVLVSAPPGYRLNIAE
                     TACDIGRFIMEKNSGVQAAAASKFEAASRHLTAALAEWRGPVLDDLRDLHFVDAFAAA
                     LIEDKVQAHTALAEAEIACGRAYAVIGELEALTVEHPYREPLWAQLITAYYLTERQSD
                     ALETYRRLKETLAEDLGIDPGPTVQALYERILRQEQLDSKKIARATAEMTIINIGQRT
                     AVSEQHAVARLREPSGRSHPLGATATRIGRLSDNDIVIDDASVSRHHAVIIDTGTSFV
                     ITDLRSANGVYVSGERVRGSASLAHGDRVRICDHDFTFEIHPSGVDRPPSGPLTIPRY
                     GSDRL"
     gene            complement(13100..13381)
                     /locus_tag="VF874_14530"
     CDS             complement(13100..13381)
                     /locus_tag="VF874_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HEX9177235.1"
                     /translation="MMRGVGVYALARLLLVAGLAALIYGVARVAGVHDFPLYIAVAFA
                     IVIALPLGIWAFAPLRRRATASVAAFDERRRRDREQLQARLRGEKTPPD"
     gene            13490..13663
                     /locus_tag="VF874_14535"
     CDS             13490..13663
                     /locus_tag="VF874_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177236.1"
                     /translation="MLFLLVILLILAGLIYAGWRVTHAARHTQPGTRVIGPDDDPDFL
                     WRLGRGNDDTTKR"
     gene            complement(13660..14787)
                     /locus_tag="VF874_14540"
     CDS             complement(13660..14787)
                     /locus_tag="VF874_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX9177237.1"
                     /translation="MPDGFDTSGFRAQQRFNEPATGPVHPSSNGRHSQPPPEWTAVTP
                     PTGFPQPAPPTWQPPPPPPTSNDLTTADLLHQTTSPPSGGWRKWLYLASFKTVNLGES
                     PKTAHRNNLVAQANRPLQGCYRIALLSLKGGVGKTTITAALGGMFATIRGDRVVAVDA
                     NPDRGTLSQKVPLETPATVRHLLRDAEGIERYSDIRSYTSQGPSRLEVLASESDPAVS
                     EAFSSADYTRTLDILERFYSLVLTDCGTGLMHSAMSAVLSKADVLVVISSGSVDGARS
                     ASATLDWLDAHGHQDMVRNSIAVINAVRPRSGKVDLHKVIDHFSRRCRAVRLVPFDPH
                     LEAGAEIDLDRLRPETRDALIELAAIVADGFPGANRREAFL"
     gene            complement(14870..15871)
                     /gene="ccsB"
                     /locus_tag="VF874_14545"
     CDS             complement(14870..15871)
                     /gene="ccsB"
                     /locus_tag="VF874_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX9177238.1"
                     /translation="MNTEHVDATLATYSDWAFTSSTLVLVGALLLLAIELAYNRSRKA
                     EQRELAGAAAAATLSRAAGVRADSAAPGIVDAPARPFDERVGRAGLALVYVGIGLLLA
                     CIMLRGFATMRAPWGNMYEFINLTCLSGLVAAAIVLRRPQYRPLWVFVLLPVLILLTI
                     SGRWLYATAAPVMPALQSYWLPIHVSVVSLGSGVFLVAGVASILFLLKMSTYGQPDTE
                     GTLARIMQKLPDAQTLDRIAYRTTIFAFPVFGFGVIFGAIWAEEAWGRYWGWDPKETV
                     SFIAWVVYAAYLHARSTAGWRDKKAAWINVVGFVAMVFNLFFINLVTVGLHSYAGLG"
     gene            complement(15868..17394)
                     /locus_tag="VF874_14550"
     CDS             complement(15868..17394)
                     /locus_tag="VF874_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX9177239.1"
                     /translation="MGTALALLFLLALGAIPGALLPQRNLNEAKVAEYIAEHPRIGPW
                     LDRLQAFDVFSSFWFTSIYVLLFVSLVGCLAPRMVDHIKNLRVTPVPAPRNLSRLPKH
                     TERQVNADATVLADTIDDRLKGWRRVARQNGYATEVSAEKGYLREFGNLVFHFSLLGL
                     LVAVAVGKLFGYEGNVIVIADGGPGFCSASPAAFDSFRAGNTVDGTKLNPICLRVNDF
                     QAHYLPSGQATSFAASIDYQAGAELNTDTWRPYRLEVNHPLRIGGDRIYLQGHGYAPT
                     FTVTFPDGQTRTQTIQWRPDDPRTLLSSGVMRFDPPAGMYPDPGERRKNQIAIQGLFA
                     PTEQLDGALLSSSFPTLNNPAVAVDVYRGDAGLDTGKPQSLFTLDPSLMHQGRLTKAA
                     RVNLRAGEQVRLDDGTLVRFDGAVPFVNLQVSHDPGQTWVLVFALMMMAGLLVSLVIR
                     RRRIWVRITPQPAGTVHVELGGLARTDNSGWGDEFERLVKRLVDADAGVAEPTREKVR
                     "
     gene            complement(17442..18320)
                     /locus_tag="VF874_14555"
     CDS             complement(17442..18320)
                     /locus_tag="VF874_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEX9177240.1"
                     /translation="MTGFAELAAAGPLLVAIGISVLAGLVSFASPCVVPLVPGYLSYL
                     AAVVGGDDETAPLGGDDETAPVGGDDETTPVGGDDETAPVGADEQPGQVGVRTARWRM
                     VGSATLFVAGFTAVFVLGTVAVLGMTTTLITNQLLLQRIGGVLTIVMGLVFVGFIPAL
                     QRQARFTPRQVSTLAGAPLLGAVFALGWTPCLGPTLTGVIAVASATDGASVARGVLLV
                     IAYCLGLGIPFVLLAFGSAGAVHGLGWLRRHTRAIQIFGGVLLIVVGLALVTGVWNDF
                     VSWVRDAFVSDVRLPL"
     gene            complement(18317..18922)
                     /locus_tag="VF874_14560"
     CDS             complement(18317..18922)
                     /locus_tag="VF874_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9177241.1"
                     /translation="MRRLIALAAVLTAVLTACSTGDDAVAQGGTFEFVAPGGQTDIFY
                     NPPESRRQPGELTGPDLMDPAKTLSLDDFRGQVVVVNIWGQWCGPCRAEITELQQVYD
                     DTHREGVAFLGIDVRDNNRDAARDFVVDRKVTYPSIYDPAMRTMIAFGGKFPTTVIPS
                     TLVLDRQHRVAAVFLRELLAEDLTPVVQRIAAEQPTKPQAQ"
     gene            complement(18910..19527)
                     /locus_tag="VF874_14565"
     CDS             complement(18910..19527)
                     /locus_tag="VF874_14565"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9177242.1"
                     /translation="MAEITTVHVMRHGAVHNPEKVLYGRLPGYRLSENGQAQAQAVAD
                     ALADRDIVLVVASPLQRAQETAKPIADRLGIDVATDHDLIESVNYFQGRRISPGDGAW
                     RDPRVWWQLRNPFTPSWGERYKDIAVRMTTAIENARAKAAGHEAVVVSHQLPVWTLRR
                     HLENKRLWHDPRQRQCNLASLTSFVFDGDRFIRVVYTEPAEQCGG"
     gene            complement(19527..20855)
                     /gene="hemL"
                     /locus_tag="VF874_14570"
     CDS             complement(19527..20855)
                     /gene="hemL"
                     /locus_tag="VF874_14570"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX9177243.1"
                     /translation="MGSAEQTTARTVASARLFAQACEVIPGGVNSPVRAFKSVGGTPR
                     FITSAQGYWLVDADRNRYVDLVCSWGPMILGHAHPAVVEAVQRAASTGLSFGAPTPAE
                     TELAAEISRRVPPAERVRLVNSGTEATMSAVRLARGFTGRPKIVKFAGCYHGHVDALL
                     ADAGSGVATLGLPSSPGVTGATAADTVVLPYNDVGAVEQAFDRFGHEIAAIITEACPG
                     NMGTVAPEPGYNAALRRITSDAGALLISDEVMTGFRISRSGWYGVDPVVADLYTFGKV
                     MSGGLPAAAFGGRAEVMERLAPLGPVYQAGTLSGNPVAMAAGLATLRAADDSVYAALN
                     RNADRLAELLHDALSEAGVPHAIPQAGNFLSVFFGDRPVNDFASAKASQTWRYAPFFH
                     ALLDSGVYAPPSAFETWFVSAALDEEAFHRIEAALPAAAQAAAKAPRPEA"
     gene            complement(21172..21720)
                     /locus_tag="VF874_14575"
     CDS             complement(21172..21720)
                     /locus_tag="VF874_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177244.1"
                     /translation="MIDTATTAVLRIARLDAVRLTDHQPHPGLCATCLRAPRAPGPVV
                     VPRMWGGFSSLNRAISSSWARCALTTPHAKRTDKAIAAAVTIHQSGRSRNHPSNSSVA
                     KSGQKKRRIIHSRFRCFAPVYEFTDYEFFEFMGEYPASRYLKRCDSRRHACLRAPVIF
                     SGSAGLTISASIGSAGACSEVS"
     gene            complement(22342..22740)
                     /locus_tag="VF874_14580"
     CDS             complement(22342..22740)
                     /locus_tag="VF874_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9177245.1"
                     /translation="MAYLKPPWFTAKIFNPIAMATGVSNTETLTVTKRASKQPQKIPV
                     LTVEVDGTAYLVSTRGESEWVKNVRANPNVTISNKSGTTGYVAREIPEQDRQGVLTAY
                     RSKAGRAVEGYFRKLPREADHPVFSLTKTG"
     gene            22764..23150
                     /locus_tag="VF874_14585"
     CDS             22764..23150
                     /locus_tag="VF874_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9177246.1"
                     /translation="MQLPQWLARFNRHVTNPVQRLWAGWLPTMGILEHIGRRSGKRYR
                     TPLTVFSTDDGVAILLTYGPNRDWLKNLTSAGRGRMQRYGKTFEVTDPRVMPKAVAAP
                     SVTGLWRPIFARLPFEYALLLKRTDR"
     gene            23147..23389
                     /locus_tag="VF874_14590"
     CDS             23147..23389
                     /locus_tag="VF874_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177247.1"
                     /translation="MTSFARRRARFNRKVTNPLLRPISGWVPAWSIVEHVGLRSGKTY
                     RTPVSVFRTDDGVAILLPYGTDTDWVRTCEPLTAVR"
BASE COUNT         3746 a         7844 c         8037 g         3821 t
ORIGIN      
        1 cgccgcagca gctcggaggc gcgaacgagg atacgccgag gatcgccgcc gaggccgacg
       61 ttcccgtcgg cgagcaacac cgtatgccag cggccagtgc cgggcagcgg gtcgaacacg
      121 tcgcggtgca acgctggcgc gccgctgcgc cgcgccagct gcaccgcggt ggccgactgg
      181 tccacgccca gcgccgggat gccacgtcgg atgaggtgcg caaccaatcg tcccgggcca
      241 caagccaagt cgattgtcgg gccgtcgcac atgcccacga cggctcggtc gaacacgtcg
      301 tcggcgcccc gcccgccgag ccagctgcgc accggcaggg ggtgcacgcg gccgtcgtcg
      361 tggcgcagcc aacatcgttt gccggtcagc gcttgctcat acaggtcgcc cagcacgtca
      421 tacccccaga gttgtgcgcg cgaatcgcga gcccgaaggg ctcacgtcgc gcaccgcggc
      481 cacgtcctcg atggtgtcga cgtcgcgcag ttcgtcgaca agcaccacgt tgattccggt
      541 gtcgcgcaac gcccgaagcg ttacagcgcc ggtgtcgcct tgcgacatcg gtaccgcccg
      601 caacacgtcg gcgtgggtgg gcgaatgcag acccagcacc caccacccgc cgtcgctggc
      661 catgcccagc accgccggtg aggctagcag ggcgtcgcaa caccgggtga gcaacgccgc
      721 ggtgacttgc ggggtgtcca tgccgatctg cagtaccggg tgcggacccg ccacggcggc
      781 ggtgtccgcg tgggcgttgg ccagccgatc ggcgaaatcc attccacgtt gcgggattac
      841 gaaataatcg gcgagtcgct cgcggatttc gtcggacctg ctggcggcgt ccagatcacc
      901 agtcagggcg accacacggg cggtcaccgg tgcggcagcc accgcgtcga gggtgtccaa
      961 taacgccgcc gcggcgatgt ccgccgccac ctcaggaccc accgctgcgg ccagccgcgt
     1021 cttcgcgacg ccggggaccg gcgctttggc gaccaccagc acggtcaccg gcacggtcat
     1081 gaaatcgccc gccagaagtc gacggccgcg accaggcttc ctctcgcgga gccgctgact
     1141 ttcgactttc cgcccgtgcg gggaccgtac cccacgtcgc gttccaccac cgtccatccc
     1201 gccgcggccg cccgtaccag tagttctacc gggtaccccg aacggcggtc ctcgacgccc
     1261 agcctcagca gggcgtcacg gcgcgcgacc cgcatcggcg cgatgtcgtg caccgaaagg
     1321 cggtgccggg tgcgcagccg ccagctcacc gccgcggtgc ccagccgcgc gtgccacggc
     1381 cagtgcagcc cgcggaccgg gcgtcgccgg ccggtcacca tgtcggcccc gcgttccagt
     1441 tcggcgacca tcagcggcag ctcggcgggg tccagcgacc cgtcggcgtc gagcaccgcc
     1501 acgatcggcg tggtcgccgc gacgacgccg gcgtgcacgg ccgacccgta gccgggcctt
     1561 tgctcggtga ccacctgcgc tccgtgcctg cgtgcgagac ccgcggtgtc gtcgtcgctg
     1621 ttgttgtcga cgacgagcgc ccgatacccg ggaggaatct tcgccagtac ccccggcagt
     1681 gacgccgcct cgttcaaaca aggcagcaca accgtcaccg agcagttggg catgtgtttg
     1741 aaggttagtc agtgagccac cgcggggtct gcgggttcag tgacaaatcg gtgacgtcgc
     1801 cgctggtcgc acggtagtca ggctaacctc ggggctgtga acgcacgagt cctgatcgcc
     1861 gacgacgacg aggtggtccg tgacgtcgtc cgccgatacc tcgagcggga cggcctcgac
     1921 gtaacggtgg ccggtgacgg cgcggaggcg ttgcgggtgc tgggcgccga gcccatcgac
     1981 gtcgccgtgc tggacgtgat gatgccgggg cccaacgggc tgaccctgtg ccggacgctg
     2041 cgcagcggcg gcggctaccc tgtcccggtg attttgctga ccgcgctgag cgaggaggag
     2101 gaccgcatcg ccgggctgga ggctggcgcc gacgactacg tgaccaaacc gttcagtccc
     2161 cgcgagctcg cgctgcgggt gcggtccgtg ctgcggcggt cctcttcggg cgcctcggcg
     2221 ctgccggtgg aagtcgccgc agaggggctg aagatgttga cggcgtcgcg ctcggcgtat
     2281 ctcgacgagc gaaaaatcag cctgacgaat cgtgaattcg atttgttgct gttcttcctg
     2341 actcacaccg atacggtgtt cacccgtgag gagctcttga agctggtgtg gcgatgggag
     2401 ttcggcgacc tgtcgaccgt gaccgtgcac gtgaagcgac tgcgcactaa acttggtgac
     2461 cgtcaccgca ttcagaccgt gtgggggcgc ggctacatgt ggggcgaacc gcatgacggc
     2521 acatgacctg ctcaggatcg ccgcgtgggc actggggtgc accgtgcccg tggtgctgtt
     2581 tggctggctg atcatccgac tggcgcggtc gtggtcgctg accgtcagca tggtggtgct
     2641 agtgctgatt ccgacgttgg cgacgctcgc cggggtgctt ggggcgtcgg gcttcatgtt
     2701 cacgcacacg ttccggtacg tcgccgttat tctggtgatc gtcgctgtcg tcaccatccc
     2761 cgccgcggtg atactggccc gattccaggc gcgaagaatg gtgtgggaga accagattcg
     2821 ggatgcagag cgtgcatcgg aggaatcccg gcggcagctg gtcgcgttcg tcagccacga
     2881 cctgcgcact ccaatggccg gcatccgcgc agtggcagag gcgatggccg acggcgtcgt
     2941 gccggaacgc gaggtccgtc agcacgcgaa acacattgag caggaatcgg ttcggttgac
     3001 cgaaatggtg aacgacctgt tcgagatgtc gaagatcaac gcgggtgcgg tggaattgcc
     3061 gcaggagaag gtggcgctgg acgaggtcgt cgacgacgtg ctcgccgcac acaagatcgc
     3121 cgcagagcac gctggcgtgg cgctgcaggc cacgctgcct gccgagccag tgcaggtgat
     3181 cggcagcgat cgcgcgctga tgcgtgtgct gtcgaacctg gtggcgaatg cgattgcgca
     3241 cacgccgtcg gggggaacgg tgacgctgac gctcggggcc gacgagaacg gtgcgttcgc
     3301 ccaggtggac gacaccggcg tgggcatcga cgagaaggac ctgccgaagg tgttcgacgt
     3361 ggcctaccgc ggctccaaca gtcgcgtgca cggaagcgac ccggcgctgc ccagcggttc
     3421 gggcctgggg ttggcgatcg ccgcgggcct ggtgcaggcc caccacggca cgctgtcggc
     3481 ccacaacctg ggcaccggcg cccggttcga ggtgaggttg ccgctcgccg actgacgctt
     3541 gtttttgagt gatctttcta ctaacgtcgt taaccatggc gaaaacaggg ttgcgcaagt
     3601 tcaagttcga gcggcaggtc ggccgggcgg tggtgaatcc gctggtcgcg gcgctggacc
     3661 ggcgcggaat acggtccaag ctggtggtcg agctggagac cacgggccgg aaatcgggcg
     3721 agccgcggca cgtgccgctc accggcagcg ctgacgaggg cggcgtctgg gtgatctcgc
     3781 aacacggaca tcgcgcgggg tgggcgcaca acatcagcgc cgatccccgg gtgcgggtcc
     3841 gggtgaacgg ccgctggcgc accggcaccg cggcgttccg gccggacgac gacgtccgcg
     3901 cacgggcccg cagcttcggc agcgggaagg tctccggcac ggcggcagcg ctcgcgatgc
     3961 gcgcgatgga gagcgatccg atctcggtgc ggatcaccta caccggctaa ggcctctcgg
     4021 aggcctgtgc aaagcgcacg atctcggcga cgacctctgg gccgtcgagc agctcgggat
     4081 cgttgtggtc ggcgcccggc accagcacga accgcttcgg ctcgggcgcc gcgtcgtaga
     4141 gccgccggct gagttcggcc ggcacgatcg agtcgcgctc gccggcgatg accagcagtg
     4201 gcgcgccgag gttttcgatg cggtcgatcg acgggtagcg atcgctcagt agttgcgcga
     4261 cgggcagccg cgggtagtgc agccggccga catccgtcaa cgaggtgaac ggcgaccgca
     4321 gcatcagcgc cgacggcgga tgctcgacgg cgaggcgcaa cgcgactgcg gcgcccagcg
     4381 attcgcccag gtatgcaatg cggtcgctat gcgcatcgag ccaggtgcgt gccgcccgcg
     4441 cgtcggcagc caagccgtct tccgagggcc gtcccggatt gccggcgtac ccgcgatagt
     4501 caaacagcaa caccgacaag ccatttcgca ccaatgcggc ggccagcggc gctcgcatgc
     4561 atcggtcccc cgcattgccg ttgcacatca acaccgccga gtgcccgtcg gccgggaaga
     4621 accatgcgcc gagccgcagc ccgtcgtcgg tgtgcagcac gacgtcttgg ccgccgcgca
     4681 acaccgtcgc cgccgaccgc actgaccccg gcgaagggaa gtagatcagc cggcgctgca
     4741 gcgcgccgat gagccgccga ggcggtaacc accgcggact ttttcgagta gaggccacgt
     4801 ggaataccgt ttcggtgcaa tcagttgcgc agcggggcgg tcgcgaactc gcggaggccg
     4861 gcctcagggc ggatgcgtgc ggtgaacccc aggatctcac gcgcacgggc ggcgtcggcg
     4921 acgatatggc ggacatcgcc gctgcggtag tagccggtga cctgtggcga cgggccgccg
     4981 ctggcctccg acaactgtgc ggctacctcg tggatcgaga tcggttgtcc ggaacacaca
     5041 ttcgcggcca agaaccgctc cgacccgaca cggttgacgg cagccacgtt cgccgccgcg
     5101 acgtcgtcga catgcacgaa gtcgcgcatc tggccaccgt cctcgaaaac ctgtggcggc
     5161 tcgccttttt cgagcgcaga ccggaagatc gcggcgaccc ccgagtacgg cgtgtcccgc
     5221 ggcatgccgg ggccgtagac gttgtggtag cgcaacgcga tgacggaccc gtcgacggcg
     5281 tcgctccagg ccagcccgta gtgctcctgc gctgtcttgc tggcggcgta caggctcctc
     5341 gggcgcagcg gcgcgtcctc gtcgaccagc cgccacgaca gctcctcacc accgagaggg
     5401 cagcggtgct cgaacacgcc cgcgtccagg tcctcacgtc ggcgcggcag cggatcgacg
     5461 tggccgtgaa cgggacagtc gtagcggccc tgcccgtaga ccaccatcga cgacgccaac
     5521 accagacgcc gaatgccggc tgcatacatc tgcgccaaca acaccgtggt cgcgtagtcg
     5581 ttgtggctgc cgtactccgg agcgtcggcg gcgtccacgc cggcgcccac catcgcggcc
     5641 tggtggcaca cgacgtcgac accggacagc agcggcgcca gcgcgtcggc gtcgcgaacg
     5701 tcgatgcgca ggcagccttc aggcgacgca ctacccggac cgtgggctgc cggcagcatc
     5761 acgtcgacgc cgacgacatc atgtccgccc gccgccaggg ccgccgccac ccgggcgccg
     5821 atgaacccgg ccgcgccggt gagcagcacc ctcacggcaa ctcgaacggc agctggacgc
     5881 cctcgtgggc caggcagtgc gtgcaggtcc gttcgctctc caccgcgcgc agcgcgtcga
     5941 gcaccacctt cttgaacgga ccgagattgc gctcgaactc ggcgaacacg tcgacggccc
     6001 gcacacccga cccgacgtcg atacccgcgt ccaggtcagt caccaacgca attgcggcat
     6061 aacacatttc gagttcacgg gccaacaccg actccggata gccggtcatg ttgatcaacg
     6121 tgaagccctg gctcgcgaac caccggctct cggcgcgggt ggaaaaccgc ggcccttgga
     6181 tgaccaccat cgtgccgccg tcgacaatgc cgggccccaa agcggcctgg cgcagcgtcg
     6241 ggcagtacgg gtcggcgaag gcgacgtgga tgccgccgga gtcgaagtag gtgtcgtcgc
     6301 gcccggaggt gcggtcgacg agctggtcgg gcaccaccgt ggcgcccggc ccgagctcgc
     6361 gcgtgagact gccgaccgcg cacggcccga agatgcgccg cacgccgagc gcccgcagcg
     6421 cccacatgtt cgcgcggtac ggcaccgcgt gcggggagaa ctggtgacgc acgccgtggc
     6481 gcggcaagaa cgcgacctcg tgatcgccga ccagcccgac tgtgattggc ccgctgggcg
     6541 gcccatacgg agtttcgacc tcgacgctgc gcacgccctc gtctaaaaac gtgtagaagc
     6601 cgctgccacc gatcactccc aacattcgcc cattgaaaca gcatgcaacg atcgacaatg
     6661 tggcgagcgt gacgcagtgg atcgagggcg cccgcccccg cacgttgccg aacgccgtcg
     6721 cgccggtgat cgcaggcacc ggcgcggccg cgtggctgca cgccgcagtg tggtggaagg
     6781 cgctgctcgc gttggtggtc gcggtcgcga tgatcgtcgg cgtcaacttc gccaacgact
     6841 actccgacgg catccgcggc accgacgacg agcgggccgg cccgctgcgt ctggtcggat
     6901 cacggctagc cacaccgcga tccgtgctcg ccgccgcggc ggtgagcctg ggcgtggcgg
     6961 cagcgggcgg tgtggtgctg gccgtggcga gcgcgccgtg gctgatcgcc gtcggcgcgg
     7021 tgtgcatcgc cggtgcgtgg ctgtacaccg gcgggtcgcg gccctacggg taccgcggcc
     7081 tcggtgaagt cgcggtgttc gtgttcttcg ggttggtcgc ggtgctcggc acgcagtacg
     7141 tgcaggcgct gcgggtcgac tgggtgggcg cggtgctggc ggtggcgacg ggctcgctgt
     7201 cgtcggcggt gctcgtcgcg aacaacctgc gcgacatccc gaccgatgcc cgcagcggca
     7261 aggtgacgct ggcggtccgg ctcggcgacg cgcgcacccg gctgctgtat cagggcctgt
     7321 tgggcgtcgc ggcgctgggc acgctggcac tgatcgcggc aaccccttgg gccgcaatcg
     7381 gtttcgtcgc aatgccgttg gccttgcgcg ctgcggcgcc ggtgcgccgc ggccaaggtg
     7441 ggcgcgagct gatcccggtg ctgcgcgata ccgggctgac gatgctggtg tggtcggtgg
     7501 ccgtcgcact cgcgctggcg ttggcggcct gactcgacgt agcggcaacg agtgtgcggt
     7561 ttcgtacgtc tagtcggggg gtcgcgtacg aaaccgcaca ctcgggttct tgagcggcgg
     7621 gcctcagttc agcgcgccgg cgtacttcgg gcagaacgag tcgaccgagg cggtgacgaa
     7681 cgcggtggcc tgacccgaac tcaggtcact gttgccgacg atctcgcggc cgatcgagtt
     7741 cggtgaattg ccttccgaca gcagctggca gacgagctga gcgttcttga ccgcagcctc
     7801 aggcgagtcg taaccgattc ccgccgcctc gatgtcgtcg aagaactggg cgttgtcgcc
     7861 ggtgagtgcc gacgccgtgg ttgccgtggc gacggcggcc agcccgaacc cgcccgcgac
     7921 gatggccgtg gcgatgacct tggtgaatcg gtggatcatg gcgctccctg tgtcggtttc
     7981 gctgatgtga caccagcgtc taccgagcgc cttggcggat tcttggaaga ttcttggcgg
     8041 ctacgtctca cgaaaaacgc aacgtctcac gaaaaacgca acagcccccg accaggcggg
     8101 ggctgtttcg tcttctcggc cgaacagcga tcagccgaac ttcagccacc agtcgtgcag
     8161 cgccaccatg tccggaccct ggccgtcggc cagcatcagg tccgcgttgc gggtccagag
     8221 catgtccggg ccgcccttat acgtgccgca cgcgacctga ccctcgactt tgtcgggggt
     8281 ggacgtgtag tgccacgtgg tcggcgacgg ggtgtcgctt cccgggcagt tctgcagctg
     8341 gtcgtcctcc ttgatcgccg tctcgaagtg ccggtcgagc gtcgcctggt cggggaacag
     8401 cgagtagcgg gcaacctcag gtccgcccgg cttggtgttc ttctcgcagt cgacgctggc
     8461 aagcgcatcg gtcacggggt cgatcggctt gcaggcaccc ggctcgtacc cgtccggaag
     8521 gatgctcatc agccgggccg acgcgccgga ctgtccggtt gtcggcggcg tcgtcctggt
     8581 ggtcgtcgtg cgcgtcttgg tatgcgtcgt ggtggtcggc gccgaggtcg tcgtcgtcgt
     8641 ggtggtggtg ttctcgtcgc tcttcgtgaa cttcggtatc agcaccacgg ccagcacggc
     8701 gagtatgacc acgaccacaa acgccacgat cgcggcgatg ataggccccc agttacgttt
     8761 gggcggctgg gccgagggca tcggccccga cggcggcggc tgccacggcg ggccgcccga
     8821 tggcggtcca cccggcccgg tgggtccgcc gggtccccac ccgcccgtgc ctcccgtgcc
     8881 cgggggctga taaggcggct ggtaaggcgg ctgggaggac gcggcgacgt agggcggcga
     8941 cgtcggcggc ggggtcgaac tcccaggcgg ggtggacgac cccggcggtg gagtggtgaa
     9001 gctcggtggc ggagtggtga agctcggcgg cgggggcgtc gccctaggcg ggggcgtcga
     9061 aaagctcggc ggcggagtgg cgaagcttgc cggcgtcgcc gacggagcga gggggccggg
     9121 catggtcgcg gcttcgctgc gggccaagat cgtgtccgcc ttgtcctgat cggggctcgc
     9181 gagcgcttcg tgtgcggccg tcgcgaggtc tcccgcggtg gcataccgat ctttcgggtc
     9241 tttggccatg ccccgggaaa ctacttcatc gaacgccgcc gggatgcctg gacgcgccgc
     9301 gctgggtttt ggaatcggct gcatcatgtg cgcggtgatc accacgctca cgctgtcggc
     9361 cggatatggc tgcgccccgg tcaggcattc gtgcagcaca cacgccagcg catagatgtc
     9421 cgcgcggtac gtcacctccc cgttggtgaa ccgctccggc gccatgtacg catacgtccc
     9481 caccgcagtg cccagctcgg tgagcttctc gtccgtcttg gcgttggcga tcccgaaatc
     9541 caccaaatac gcgaagtcat cccgggtgat caaaatgttc tccggcttga cgtcacgatg
     9601 caccaggccg ctctcgtgcg cggcgtccag cgccgaagcc acctggcgga tgatcgccac
     9661 cgcccgcgcc ggcgtcagcg gcccgtaccg gaccaacatc ttgcgcaggt ccgtaccgtc
     9721 gatgaaccgc atgtcgacgt acaggacacc gtcgatttcg ccgtagtcgt gaatcggcac
     9781 cacatgcggc tcctgcaacc ggcccgccga atgtgcctcg cgctgtaggc gtttgcggaa
     9841 caccggatcg ttcgacatgg tggccggcag taccttcagt gccaccgtgc ggtccttgcg
     9901 ggtgtcttcg gcctcgtaga cctcgcccat cccgccctta ccgagcaacc gtcgcaagtg
     9961 ataatgaccg aaatcagtac ccacccgcga gtcggtgtcg ctcattggtc tcctaaccct
    10021 cccgcgggcc catcacgggc cgctgcccac tgcgggaggg ggcacacccc gccgcaagac
    10081 gtccacacct ctttgtgttg ccgggtacaa cgtaatgcgt tttcaaccgg atcacagcgg
    10141 aaccgtccta ctgtgccgcc gttgccggcc cctcagtcag tcgagcactt cgaaatcagg
    10201 atcgaaccgc cgttgttttg agctagaggg cgtccttttc ggcgtcctcg atggtcgcac
    10261 cgagcccgcc gtgccaggtg gtgtcgctct gggcgagcgc cgcacatcca tctgttgcgt
    10321 aggccgccac ctggcaatcg gtaccgccgt actgtttgca gtagtccgtc gccgtgttgg
    10381 tggcttgatc ctggctggtc gcgttgttcg accaaccgaa cgctcccgtc gcaggcgaga
    10441 acacgatgac gacccacatt ccgctcgtat tgaccggcgg ttgcgaggtg gccgtggtcc
    10501 tggttgtcgt cgtccgcgtc ctagtggtcg tcgtgtcctc agacggcgtc gtcgtatcag
    10561 tagcggcgtt gttgtttttg tcgtctgagc cgctcatcaa caacacggcg gtgaccacgc
    10621 ctatgagaac aacgacgaac gccgcgagcg cggcgatgat cggcagatag ttgcggcgtt
    10681 tcggcggcgg agccgacggc atcggaccgg acgctgaggt ctgccacggc ggcggaccgc
    10741 ccggtccacc cggaccggag gaagccgtgg tacccggcgg ctggtagggc ggttgtgagg
    10801 acgcggcgac gtaggggggc ggcgtcgacg gcggcggggt tgaggaccca gggggcggcg
    10861 tcatgaacct cgccggcggc ggggttaccg acccgggcgg tggtgtcgta atccccggcg
    10921 gcggagttat cagaccaggg agggtggccg ccgcgctgcg ggacaggatg ttgtccgacg
    10981 tgtccctgtc ggcgctcgcg agcgcgtcat gtgccgcagc cgcgagatca cccgcggtgg
    11041 cataccgatc tttcgggtcc ttggccatgc cccgggagat caccgcatcg aacgcctgcg
    11101 gaacgcccgg acgcaccgca ctcggatgcg gaatcggctg catcagatgc gcggtgatca
    11161 gcacgctcac gctgtcggcc ggatagggct gcaccccggt caggcactcg tgcagcacgc
    11221 acgccagcgc gtagatgtcg gcgcggtacg tcacctccga gttcgaaaac cgctccggcg
    11281 ccatgtaggc atacgtgccc accgcggtgc ccagctcggt gagcttctcg tcggtggtgg
    11341 cgttggcgat cccgaaatcc accaggtacg cgaagtcatc ccaggtgatc aaaatgttct
    11401 ccggcttgac gtcacgatgc accaaaccgc tctcgtgcgc cgcgtccaac gccgacgcga
    11461 tctgacggat gatcgccacc gcccgcgccg gcgtcagcgg cccgtaccgg accaacacct
    11521 tgcgcagatc cgtgccgtcg atgaaacgca tgtcgacgta aaggacaccg tcgatttcgc
    11581 cgtagtcgtg aatcggcacc acatgcggct cctgcaaccg gcccgccgaa cgcgcctcgc
    11641 gctccaggcg tttgcggaac accggatcgt gagacatcgc cggcggcagc agcttcaacg
    11701 ccaccgtgcg gtccttgcgg gtgtcctcgg cctcgtagac ctcgcccatc ccgcccttac
    11761 cgagcacccg tcgcaagtga tagtgaccga agtcggtacc cgcccgcgac tcgctgtcac
    11821 tcattcgtcg tctcaaccct tccgcagact cgcaccttgc ccgtcacgct aacgtctctt
    11881 gagtcagatc acagccgatc cgacccgtac cgcgggatgg tcagcggtcc gcttggcggc
    11941 ctgtcaacac cgctggggtg gatctcgaac gtgaagtcgt ggtcgcagat tcggacccgg
    12001 tcaccgtggg ccagcgaggc actcccgcga acgcgctcgc cggatacata gacgccgttg
    12061 gccgacctca ggtccgtgat cacgaagctg gtgccggtgt cgatgatgac ggcgtggtgt
    12121 cggctgacgc tggcgtcgtc gatgacgatg tcgttgtcgg acagccggcc gatccgagtg
    12181 gcggttgcgc ccagcggatg agagcgcccg gacggttcgc gcaggcgcgc aaccgcgtgc
    12241 tgctcactga ctgccgtgcg ctgaccgatg ttgatgatcg tcatttcggc agtggccctg
    12301 gcgatcttct tgctatccaa ctgctcctgc cgcaggatcc gctcgtacag cgcctgcacc
    12361 gtcgggccgg gatcgatgcc aagatcctcg gccaacgtct ccttgagtcg gcggtaggtc
    12421 tcgagggcgt cggactggcg ctcggtgagg tagtaggcgg tgatcagctg cgcccacagc
    12481 ggctcgcggt acgggtgctc gacggtcagc gcttcgagtt cgccgatcac ggcatatgca
    12541 cggccgcagg cgatttcggc ttccgctaac gcggtgtgcg cctgcacctt gtcctcgatg
    12601 agcgcggcgg cgaacgcgtc gacgaaatgc aagtcgcgca gatcgtcgag caccggccca
    12661 cgccactcgg ccaacgccgc tgtcaggtgc cggctcgccg cctcgaactt gctcgccgcg
    12721 gcggcctgca ctccggagtt cttctccatg atgaatctgc cgatgtcgca agctgtttca
    12781 gcgatgttga gccgatatcc cggcggtgcg ctgaccaaca ctgcgcgcgg gtcgacaccg
    12841 gacttggcca gcagccggcg caggttcgac acgtacgagt gcaggctggc ccgcgcctcc
    12901 gccggcggcc actgctccca cgcggcggtg atcagcgcat cgatgctcac cggccggttg
    12961 cggtttatca ccagcatggc cagcaccgcg cgctgtttcg gagtgcccaa cgacaccggc
    13021 gtcccgtcga cggtcatctg caatggaccc aggacaccga atcccagacc ggtctgcgtc
    13081 atggcaccat cgtcctccgc taatcgggcg gtgtcttctc gccacgcagc cgcgcctgca
    13141 actgctctcg gtcacggcgc cggcgttcgt cgaacgcggc gacgctcgcg gtggcccgcc
    13201 ggcgcagcgg agcgaatgcc cagatgccca gcggcagcgc gatcacgatc gcgaatgcga
    13261 ctgcaatgta gagcggaaag tcgtggacac ccgcgacgcg cgcgacaccg tagatcagcg
    13321 ccgccagacc cgcgacgagc agcagccggg ccagcgcgta gaccccgaca ccgcgcatca
    13381 tgctcggacc gactccgttt tgcggcaccg ttgctgtctc ctcttcgcgc aagcgctcat
    13441 cggcctgagc ctaaccacgc gcgtatattc aagccaggag gtgttcacgc tgcttttcct
    13501 gctcgtcatc ctgctcatcc tcgccggact catttatgcc ggttggcggg tgacgcatgc
    13561 cgcacgtcac actcagcccg gcacgcgcgt gatcggccct gacgacgatc ccgatttcct
    13621 gtggcggctg ggccggggca acgacgatac gaccaagcgc tagaggaacg cctcgcgccg
    13681 gttcgcgccc ggaaagccgt cggccacaat ggcagccagt tcgatcagcg cgtcccgtgt
    13741 ttcgggcctc agccgatcga ggtcgatttc tgcgcccgct tccagatgag ggtcgaacgg
    13801 caccaaccgc acggcacggc agcgccggga gaagtggtcg atcaccttgt gcaggtcgac
    13861 cttgcccgag cgcggtcgca ccgcgttgat caccgcgatc gagttccgca ccatgtcctg
    13921 gtggccgtgg gcgtccagcc aatccagcgt cgccgacgcg ctgcgggcac cgtccacgga
    13981 gcccgagctg atcacgacca gcacgtcggc cttggacagc acggccgaca tcgccgaatg
    14041 catcaggccg gtgccgcagt cggtgagcac caggctgtag aagcgttcga ggatgtcgag
    14101 cgtgcgggtg tagtccgcgg agctgaacgc ctccgagacc gcggggtcgc tctccgacgc
    14161 cagcacctcc agcctgctgg gtccttgtga cgtgtagctc ctgatgtcgc tgtagcgttc
    14221 gatcccttcg gcgtcgcgca gcagatgccg cacggtggcc ggggtctcca gcggcacctt
    14281 ctggctcagc gtgccgcggt cggggttggc atcgacagcg acgacgcggt cgccgcggat
    14341 tgtcgcgaac atcccgccca acgcggcggt gatcgtcgtc ttgccgacgc cgcccttcag
    14401 cgacagcagc gcgatccggt agcagccctg cagcggccgg ttggcctgcg caacgaggtt
    14461 gttgcggtgc gcggtcttgg ggctttcgcc gaggttgacc gtcttgaacg aggccagata
    14521 cagccacttg cgccagccgc cggacggcgg ggatgtcgtc tggtgcaaca ggtccgcggt
    14581 ggtcagatcg ttggaggtgg gcggcggtgg cggcggttgc caggtgggcg gggcgggctg
    14641 cggaaatccg gtgggcgggg tgacggcggt ccactcgggc ggcggttggc tgtgccggcc
    14701 gttgctgctg gggtggacgg ggcctgtggc cggctcgttg aagcgttgct gtgcgcgaaa
    14761 gccgctggta tcgaagccgt caggcacgcg ctgtccccct tggttattgg ctcttcgcgc
    14821 aagcgctcat cgctgttatt ggcttactcg gtcgacacta gcgcagtggc tagcccaagc
    14881 cggcatacga atgcaggccc acggtgacca aattgatgaa gaacaagttg aacaccatcg
    14941 cgacgaaacc gacgacgttg atccacgccg ctttcttgtc gcgccagccc gcggtggagc
    15001 gggcatgcag gtacgccgcg tacacgaccc acgcgatgaa cgacaccgtc tccttggggt
    15061 cccagcccca gtaccgaccc caggcctcct cggcccagat cgcgccgaag atcaccccga
    15121 agccgaacac cgggaatgcg aaaatggttg tgcggtaagc gatccggtcg agggtctggg
    15181 cgtcgggcag cttctgcatg atgcgggcca gtgtgccctc ggtgtcaggc tggccgtatg
    15241 tggacatctt cagcaggaac aggatgctgg ccacgccagc gacgaggaac accccggagc
    15301 cgaggctgac caccgatacg tggatcggca gccaatacga ttgcagcgca ggcatcacgg
    15361 gcgcggcggt ggcgtaaagc cagcggcccg aaatcgtcag caggatcagc accggcagca
    15421 gcacgaacac ccacagcggg cggtactgcg gacgccgtaa cacgatggcg gcggccacca
    15481 gccccgacag gcatgtcagg ttgatgaact cgtacatgtt gccccacggc gcccgcatgg
    15541 tggcgaaccc gcgcagcatg atgcaggcga gcagcagccc gatgccgaca tacaccagcg
    15601 cgaggccggc gcggccgacg cgttcgtcga acgggcgcgc tggcgcgtcg acgattccgg
    15661 gggcggcgct gtcggccctg acgccggccg ctctgcttaa tgtcgccgcc gctgcggctc
    15721 cggcaagttc gcgttgttcg gccttgcggc tgcggttgta ggccaactcg atggccagca
    15781 gcagcagggc gccgaccagc acgagggtcg acgacgtgaa cgcccagtcg gagtaggtgg
    15841 ccagtgttgc gtcgacgtgc tcggtattca tctgaccttc tctcgcgtcg gctcagcgac
    15901 cccggcgtcg gcgtcgacca gccgcttgac gagccgctcg aattcgtcgc cccagccgga
    15961 gttgtcggtg cgggccaggc cgcccagctc gacgtgtacg gtaccggccg gctgcggggt
    16021 gatccggacc cagatccgtc gtcgtcggat caccagcgac accagcagcc cggccatcat
    16081 catcagcgcg aacaccaaaa cccaggtctg gcccgggtcg tgggagacct ggaggttcac
    16141 gaacgggacc gcaccgtcga agcgcaccag cgtgccgtcg tcgagccgga cctgctcgcc
    16201 cgcgcgcagg ttgacccgcg cggccttggt gagccggcct tggtgcatca gactcgggtc
    16261 gagcgtgaac agcgattgcg gcttaccggt gtccaggccg gcgtcgcccc ggtagacgtc
    16321 gacggcgacg gcggggttgt tcagtgtggg aaagctcgac gacagcagcg ctccgtcgag
    16381 ctgctcggtg ggcgcgaaga gtccctggat cgcgatctgg ttcttgcggc gctcgccggg
    16441 gtcgggatac atgcctgcgg gcgggtcgaa ccgcatgacg cccgacgaca gcagcgtgcg
    16501 cggatcgtcg gggcgccact ggatggtctg cgtgcgagtc tgcccgtccg ggaacgtcac
    16561 cgtgaacgtc ggcgcatagc cgtgaccctg caggtagatc cggtcaccgc cgatgcgcag
    16621 cgggtggttg acctcaagcc ggtacggccg ccacgtgtct gtgttcagct cggcgccggc
    16681 ctggtagtcg atgctcgcgg cgaacgaggt ggcctgcccc gacggaaggt agtgcgcctg
    16741 aaagtcgttg acacgcaggc agatcgggtt cagcttggtg ccgtcgacgg tgttgccggc
    16801 gcggaacgag tcgaacgccg ccggtgacgc cgagcagaag cccggaccgc cgtcggcgat
    16861 aacgatgacg tttccctcgt agccgaacag ctttcccact gcaacggcga ccagcaggcc
    16921 cagcagtgag aagtggaaca ccaggttgcc gaactcgcgc aggtagccct tctccgcgga
    16981 aacctctgtg gcgtagccgt tttgacgggc gacgcgacgc cagcccttca gccggtcgtc
    17041 gatggtgtcg gcgagtacgg ttgcgtcggc attgacctga cgttcggtgt gtttgggaag
    17101 tcgggacagg ttgcgcggcg ccggcacggg cgtcacgcgc aggttcttga tgtggtcgac
    17161 catccggggc gccaggcatc cgaccaatga cacgaacagc aacacgtaga tggacgtgaa
    17221 ccagaagctg gagaacacgt cgaacgcctg cagccggtcc agccatgggc cgatcctcgg
    17281 gtgctcggcg atgtactcag cgaccttggc ctcgttgagg tttcgctgag gcagcagcgc
    17341 accggggatc gcgcccagcg cgagcaggaa cagcagtgcc agcgcggtgc ccatcgacgt
    17401 cagcgcccgc caggtgttgc gaacccatgc gacgacgcgc ctcacagcgg cagcctcacg
    17461 tcggagacga acgcgtcgcg cacccacgag acgaagtcgt tccacacccc ggtgaccagc
    17521 gcgagaccga ccacgatcag cagcacgccg ccgaagatct ggatcgcgcg ggtgtggcgg
    17581 cgcagccagc cgagcccgtg cacggcgccg gccgacccga acgccagcag cacgaacggg
    17641 atgcccagac cgaggcagta cgcgatcacc aacagcacac cgcgcgcgac gctggcgccg
    17701 tcggtggccg acgcgaccgc gatgacaccg gtcagcgtcg gccccagaca cggtgtccag
    17761 ccgagcgcga acaccgcacc gagcagcggc gcgccggcca gcgtcgacac ctgacgcggc
    17821 gtgaacctgg cctgccgctg cagcgccggg atgaagccga cgaacaccag tcccatcacg
    17881 atcgtcagca cgccgccgat gcgttgcagc agaagctgat tggtgatcag cgtcgtcgtc
    17941 atccccagca ccgctaccgt gccgagcacg aacaccgcgg tgaacccggc cacgaacagc
    18001 gtcgccgagc ccaccatccg ccaccgcgcg gtccttacgc ccacctgccc gggttgctcg
    18061 tcggcgccga ccggtgccgt ttcgtcgtct ccgccgaccg gtgtcgtttc gtcgtctccg
    18121 ccgaccggtg ccgtttcgtc gtctccgccg agcggtgccg tttcgtcgtc tccgccgacg
    18181 acggccgcca gatacgacag gtagcccggc accagaggca ccacgcacgg cgacgcgaac
    18241 gacaccagcc cggcgagcac agagatgccg atcgcgacga ggagcggtcc ggcggcggcg
    18301 agttcggcga aacctgtcac tgcgcttggg gtttcgtggg ctgctcggcc gcgatccgct
    18361 gcaccactgg cgtgaggtct tcggccagca gttcacgcag gaacaccgcg gcgacgcggt
    18421 gctgccggtc cagcacgagc gtggacggga tcaccgtggt ggggaacttg ccgccgaatg
    18481 cgatcatcgt gcgcatagcc gggtcgtata tcgacgggta ggttaccttg cggtcgacga
    18541 cgaaatcgcg tgcggcgtcg cggttgttgt cgcgcacgtc gatgccgagg aacgcgacgc
    18601 cctcgcggtg cgtgtcgtcg tacacctgct gcagttcggt gatctcggcg cgacacggtc
    18661 cgcaccactg gccccagatg ttgacgacca ccacctggcc gcgaaagtcg tccagcgaca
    18721 acgtcttcgc cgggtccatc agatccggcc ccgtcaattc acctggctgg cggcggcttt
    18781 cgggcgggtt gtagaagatg tcggtttgcc cgccgggcgc gacgaactcg aatgtgccgc
    18841 cctgcgcgac ggcgtcgtcg ccggtggagc aggcggtgag cacggccgtg agcacggccg
    18901 caagagcgat cagccgccgc attgctccgc cggttcggta taaacaacgc ggatgaaccg
    18961 gtcgccgtcg aagacgaacg acgtcagcga cgcgaggttg cactgccgct gccgcgggtc
    19021 atgccagagg cgcttgttct cgaggtggcg gcgcagcgtc cacaccggca gctgatggct
    19081 cacgaccacc gcctcgtgcc cggccgcctt cgcgcgcgcg ttttcgattg cggtcgtcat
    19141 acgcacggcg atgtccttat agcgctcgcc ccatgacggc gtgaacgggt tgcgcagctg
    19201 ccaccacacg cgcgggtcgc gccacgcgcc gtcgccgggc gaaatacgtc tgccctgaaa
    19261 gtaattcacc gactcgatca ggtcgtggtc ggtcgccacg tcgatgccga ggcgatcggc
    19321 gatcggcttc gcggtctcct gcgcccgctg cagcggtgag gccaccacga ggacgatgtc
    19381 gcggtcggcc aacgcgtcgg cgactgcctg cgcctgggcc tggccgttct ccgacagccg
    19441 atagccgggc agccggccgt agagcacctt ctcggggttg tgcactgcgc cgtggcgcat
    19501 cacgtgcacc gtcgtgatct ccgccatcac gcctccggcc gaggggcttt cgcggccgcc
    19561 tgggcagcag cgggcagcgc ggcctcgatg cggtgaaacg cctcttcgtc caaagccgct
    19621 gagacgaacc atgtttcgaa cgcgctcggg ggcgcgtaga caccgctgtc gaggagcgcg
    19681 tggaagaacg gcgcataccg ccacgtctgg ctggccttgg ccgaggcgaa gtcgttgacc
    19741 gggcggtcgc cgaagaacac cgacaagaaa ttacctgcct gcggaatagc gtgcggcaca
    19801 ccggcttccg acaatgcatc atgcagcagc tcggccagcc ggtcggcgtt gcggttcaac
    19861 gcggcgtaga cggagtcgtc ggcggcgcgc agcgtcgcca acccggccgc catcgcgacc
    19921 gggtttcccg acagcgtccc ggcctgatac accggcccca gcggcgccag gcgctccatg
    19981 acctcggcgc ggccgccgaa cgccgcggcg ggcagcccac cgctcatcac cttgccgaac
    20041 gtgtacaggt cggcgaccac cggatccacc ccgtaccagc cggagcggct gatccggaac
    20101 ccggtcatca cctcgtcgct gatcagcagc gcaccggcgt cggaggtgat gcggcgcagc
    20161 gccgcgttgt agccgggctc gggtgcgacg gtgcccatgt tgcccgggca cgcttcggtg
    20221 atgatcgccg cgatctcgtg cccgaagcgg tcgaacgcct gctcgacggc gccgacgtcg
    20281 ttgtacggca gcacgaccgt gtcggcggcc gtcgcaccgg tgaccccggg cgaggacggc
    20341 agacccagcg tcgccacccc ggacccggcg tcggccagca gcgcatcgac gtggccgtgg
    20401 tagcagcccg cgaacttgac gatcttgggc cggccggtga acccgcgggc cagccgcacg
    20461 gcgctcatgg tcgcctcagt gcccgagttc accaggcgga cccgttcggc cggcggcacc
    20521 cggcgactga tctcggcggc aagctcggtc tccgccggcg tgggcgcgcc gaagctcagg
    20581 cccgtcgagg cggcgcgctg cacagcctcc accaccgcgg gatgcgcgtg gccgaggatc
    20641 atcgggcccc acgagcagac caggtcgacg tagcggttgc ggtcggcgtc caccagccag
    20701 tagccctggg ccgacgtgat gaaccgcggc gtgccgccca cggatttgaa tgcccgcacc
    20761 ggcgagttca ccccgccggg gatcacctcg catgcctgcg cgaacagccg ggccgaggcg
    20821 acagtcctcg cggtcgtctg ctcagcgctg cccatgggtc tagtgtccca gccgctgcgc
    20881 aggcgtcgac tacagggtgt agtggaaaag gtagcgctgt gctgtgagtg tgattaccct
    20941 ggccgcgtct gaacgctgta ccggtgattg ttcgacagtc cgctacggcc tcgacagtcc
    21001 cccgagagga gacacgatca tcatggcaca caccaccgac gccgggcccg ccacgccctc
    21061 ggcgagccga cgatgaccgc gtgggcgatc gcgcaccagt tcgccaaggc caaggaactg
    21121 tcgtactggg tcgccggcgg catcttcaac ggcgccgacc ggtgtagtga atcacgacac
    21181 ctcggagcaa gccccggccg agccgatcga cgccgagatc gtcaagccgg ccgagccgga
    21241 gaagatcacc ggggcacgca gacaggcgtg ccgccgcgag tcgcaacgct ttaggtaacg
    21301 ggaggctggg tactctccca tgaactcgaa gaactcataa tccgtgaact catagaccgg
    21361 cgcgaaacag cgaaatcggg agtggatgat gcggcgcttt ttctggccac tcttggcgac
    21421 gctgctgttg gagggatggt tcctcgatct tccgctttgg tggatcgtta cggcggcagc
    21481 gatagcctta tccgtccgct tggcgtgtgg agtagtcaaa gcgcaccgcg cccaggagga
    21541 actgatcgcc cggttcaacg acgaaaatcc gccccacatt cggggcacca caactggccc
    21601 gggtgcgcgg ggcgcgcgca ggcaggttgc acacaatcct ggatggggct ggtgatcggt
    21661 caggcgcact gcgtcgagcc tcgcgattcg gagcacggcg gtggtggcgg tgtcgatcat
    21721 gcgtcgcacc tttactccac tcgtgagttc tgagaagaag tgctttcgaa cgctccaacg
    21781 cgtcgatttg caggtagggg aggagcctcg gatcgagttc ggccggatcg aactcggcca
    21841 tcaacacggc gatgtcctcc tcgttacggc cgtatctgct ggcgacagcc gacgctttcg
    21901 ccaggtcggt atagcgtgct gagcggtctt ggtgcgggtc ggacgagatc ccggatcccc
    21961 tgttggacaa agaacttcac gatggtgaac aaattgttgg gtggaacgat ccgttcttcg
    22021 tttgtgggtc tttgagaaag aagctgttca gattttcggg ctcgttcaag gcgcgtcaac
    22081 acggcgccgg acgccgaaaa tctccgatcg cgcgcagccc tctgatgtgg cacaggcttg
    22141 tgccgacctg ctgcgcgggc tctttcgccg gtccacgccc gaacgaggcg gcgcgcaaca
    22201 atcaggccgt caaacccctg ccgtaccgca agcaacgggc ccgctggccg ctcacctgtc
    22261 ggggtcgtac ttgccggggt cggcgagcac agtgggcggt cggccgttgg tgaagccttg
    22321 acgtacaggc gccttcatgc gtcagcccgt cttcgtcagg ctgaacaccg ggtggtcggc
    22381 ctcgcgcggc agtttccgga agtagccctc gacggctctg ccggccttgc tgcggtatgc
    22441 ggtcaagaca ccctggcggt cctgctcggg gatttctcgc gcgacgtatc cggtggtacc
    22501 cgacttgttg ctgatcgtga cattcgggtt ggcccggacg ttcttcaccc actccgactc
    22561 gccacgcgtg gacaccaggt acgcggtgcc gtcgacctcg acggtgagca ccggaatctt
    22621 ctgcggctgc ttgctggcgc gtttggtcac cgtgagcgtc tcggtgttgc tgaccccggt
    22681 cgccatcgcg atcgggttga agatcttcgc ggtgaaccac ggcggcttga ggtaggccat
    22741 ggtctacgag tttgatggtc ggtatgcagt taccgcaatg gctggcccgg ttcaatcggc
    22801 atgtcaccaa tccggtgcaa cggctgtggg cgggctggct gccgacgatg ggcatcctcg
    22861 agcacatcgg gcggcggtcc ggcaagcgct accgcacccc gctgaccgtg ttcagcaccg
    22921 acgacggcgt ggcgatcctg ctcacctacg ggcccaaccg ggactggctg aagaatctga
    22981 cgtccgcggg gcgcgggcga atgcagcgct acggcaagac gtttgaggtc accgatccgc
    23041 gggtgatgcc gaaggcggtt gcggcaccgt cggtcacggg tctgtggcga ccgatcttcg
    23101 ctcgattgcc gttcgagtac gcactgctgc tgaaaaggac tgacagatga cgagtttcgc
    23161 aagacgacgg gcgcggttca accggaaggt caccaatccg ctgctgcggc cgatcagcgg
    23221 ctgggtgccg gcgtggtcga tcgtcgagca tgtcgggcta cgctccggca agacctaccg
    23281 aaccccggtg tcggtcttcc ggacggacga cggcgtcgcg atcctgctgc cctacggcac
    23341 cgacaccgac tgggtgagaa cgtgcgagcc gctaacggcg gtcaggtgaa gttgagcggc
    23401 aagacatttc gggtgacgag cccgcggatc gtgccgaccg ccgatgcg
//
LOCUS       DASWBF010000389         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_366590,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000389 DASWBF010000000
VERSION     DASWBF010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366590"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(562..2119)
                     /locus_tag="VF874_14595"
                     /pseudo
     CDS             complement(562..2119)
                     /locus_tag="VF874_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216546.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="erythromycin esterase family protein"
BASE COUNT          350 a          714 c          685 g          370 t
ORIGIN      
        1 gaagagtcca tcgctgaccc ctatgaactg ctccatccgc tcggccgtca gccgtgccga
       61 ccacggccaa ctgaccggac tggtccttcc gggcagaaaa tgagcggcgg caccgcgcgg
      121 cgacctcgcg gagctgctcc acgtcagggt ggcggccccg ccagcctcaa gacttcccta
      181 actggcttaa ccccgccgcc agcacgggcg cggtgcgcag ccgcaccttc tcccagtgct
      241 ttatggcgtc gcctgctgtc gaatgcgcca acggtagagc tcgtcggtgc cccacaccgc
      301 aaacggcatc gggataacca tgagcagcat ccacccggtg agcgccgctg tgccgaatat
      361 ggatagcagc gcgggcagat agatgacagt ggctgcaaag aacaagccag ccgtgcggcc
      421 accgcccggg tcgtatcggg gtgcccggca ccgcggtttg cccgtgggca gcacccactc
      481 ggtatcggtg gtgatgtcca gggctgggtt catcgtcggg gtcacaatgg tggggcgcgt
      541 ggtgggcgtg ctcatgggtt cgatcgtctt tggataggtc tcgggtgtct ctccggcgat
      601 ccacacactg gtcggctcca gcggctcaag ggcccgggtg ttctcgatgt ggatcatcgc
      661 gtcgaactgg ccggagggcc ggacgtggta ataatggctc tgccgttcgg tggccggctg
      721 atagatgacg ccgatggctc ggcccagccg gggcacgtcc agcgcctcgg tcgccgacat
      781 gtcatgcatc ggtaccagga aagcgtccct gcctgcctcg tggaacagtt cctccacgct
      841 gccgttcagc gcgggccgga cgaccttgcg ttcggcgatg ccgccccatt cgctggcagc
      901 ggtgacagtg ccggtgtacg tgctgaaccc gatcaggcgg caatcgccgt ggcgctcacg
      961 gacaagctgg cccagggtca gctgaccatc ggccgacacc gccgtcgcgc gtgcgtcgcc
     1021 gacatgagag ttgtgcgccc acaccacgat tcgtgccggt tgggtaccgg gatggcgact
     1081 catgtgagca agcagcgcct ccagcgtctg agccatgtgc gtgtcccgca ggttccacga
     1141 cgtgacgcgt ccgctgaaca tcgcgcggta gtaaacctcc gcgtcgcgta ccgtctgggc
     1201 gttctgttcc gcgtagaaca tttcgtcttc ggcaagcaga ccgtcgcggc gcgcgtaggc
     1261 cagcgcattg cgctggatct cgacgagttg ttcgatcgct tcgcgttcac acgacaaccc
     1321 ggcgccgaag gcggcggcga atccgtacgc ctgcccgtcg tcgtcggcgg acgcatgatc
     1381 gaaacacgcg taacgctgac gcgctcgggc ggccgccacc ggatccactt tgtcgaggta
     1441 gacgatgacc tcccgcatcg accggtgcag gctgtacagg tccaggccgt aaaagccggt
     1501 ttgccgccgg ccgtccacct ggcaacggtt gttgtgcgag cgcaaccatt cggcgaagtc
     1561 ccgaaccacc gcattgcgcc acatccacgc ggggaagcgc tcgaacccgc gcagcgcctc
     1621 ctccgccgtc gtgtcgtcgc cgaggccgcg gacgtatcgg ttgactcgat aggcatcggg
     1681 ccaatccgcc tcggccgcga ccccgcagaa gcctttctcc tcgatcaacc acttggtgat
     1741 ctcggcgcgg gcctcgtaga actcgtgggt cccgtgagag ctttcgccga tcagcacgat
     1801 cttggcgtcg cctacgagct cggacaagac ctcgcgcggc ggcacgccgc ccggggcgtc
     1861 gatggcgaca cgccggatga cgtcggccgg ggtttcggcg actaccgtcg tcgtcggcgc
     1921 aggcgccaaa gtggttggtg tggcgagcag ttcgcgtact tcctgatcgc tgacctgact
     1981 gaaatcccaa aacgattccc cgacagccag aaatggtgta ggcatcgtcg cgcacaccac
     2041 gtcctcgacg atccccacga actcgcggac cgtcgactcg ggagccgccg gtacggcgat
     2101 cacgatctcc gcgggctct
//
LOCUS       DASWBF010000390         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_298187,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000390 DASWBF010000000
VERSION     DASWBF010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298187"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..618)
                     /locus_tag="VF874_14600"
                     /pseudo
     CDS             complement(265..618)
                     /locus_tag="VF874_14600"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1146..2126)
                     /locus_tag="VF874_14605"
     CDS             complement(1146..2126)
                     /locus_tag="VF874_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9177248.1"
                     /translation="MGVTVAVTGPTGEIGLAAVTALERDPAVDRIIGMARRPFDPSTH
                     GWTKTTYRQGDILDRDAVDALVADADVVVHLAFIIMGSREESSRINLAGTRNVFEATV
                     AAERPRRLVFTSSVAAYGYHPDNPVPLTEDVPTRGSPEHYYSAQKAACEAVLVELTAG
                     SSLEVFILRPCIVAGPHAKVLADTMPWHRLPAPVRVAMRVVPILKPVLPDPGYPLQLV
                     HHDDVAAAIALAATSPAPPGAYNIAGDGVITLSDVATALGGRGVRVPTAAATAASAVI
                     AKMPCVPSALEWINVARTSMVMDTSKAKTKLGWQPTYSSAETLQALAEAL"
BASE COUNT          395 a          777 c          728 g          356 t
ORIGIN      
        1 cccaggcccg agaacgcctc gaacagttac acgcccgggg tcgcgaagac ctctgacaac
       61 cgccagttga tcacctcgcc tccgcgacaa tggcggtccc ttctacgtgg caaacgacac
      121 cccaggcaat agaactcaca aacccgtcct cgccctgacc aaccagccaa gccacgttga
      181 aattacgaaa ccatcacgcg ttgaacgcgt gtcgtcgacc acgggccctg gtcgcggcta
      241 ggcccgccat cgtgcgctcg tgcatcagtg cgcgctcgat tccgcgagcg caccgaacac
      301 atgcagcacc agcctgccgg cggcgctggt ggtatcgatc tgctcggcca atgagcgcag
      361 cccgacaccc cgcagcgaca ggtcctcgac gagcgccaat agcaaggatc ggcccagccg
      421 atccaggtgg cacaccacca cggtgtcgcc ggtcgacgcc gcctcgacgt gctcggccag
      481 cgccggccgg tgtgttcaca ccccggaagc gacgtcggtc cagatccgct caaccccgcc
      541 cgatcggagc gcgtcgagtt gcccctcaac actctgatca gccgtcgaca cccgtgcata
      601 ccccagcagt tcacccatgc cggcagattt actctccccc accgacacaa actggacagg
      661 tttaccgaac acaaacgccg caggtcacgg cggcacgtcg aggctgtctc gaaaacgttc
      721 gttatcggga cgactcggcc gcctccagga atccagaggc tagttgtctt cgaagaagcg
      781 ggccgccagg tcgcggatgc gcttgagccg tttggagacc gcactgtggt tgctgtgccc
      841 attatggcgg cgatctcggt gaggttggtg gtgctgttgc gcagcagcac gacaacctcc
      901 cggtcgagtg ggttgagcag tgtcatgaag tcgctcactg cgaggccgtc gacgatctcg
      961 gtctcggggc cgtggaccgg gatggtgtcg gtcagctcga cgaaggtgac cttctccccc
     1021 ggcgatcaac gccgcctact gggacgactg cctgcggacc ggtttttgtc tcggttggga
     1081 caacgtcggg gaccttacgc aattcgagaa gtaggcaagc gttcggtcag ccgacgacgg
     1141 cgcgctcaga gggcctcggc gagggcttgc agcgtctccg cggagctgta cgtcggctgc
     1201 cagccgagct tggtctttgc cttgctggtg tccatgacca tcgatgtccg ggccacgttg
     1261 atccactcca gcgcggacgg cacgcatggc atcttcgcga tcaccgccga ggccgcggtc
     1321 gcggccgcgg tcggcacccg cactcctcgc ccgcccagcg cggtggcgac gtccgacagg
     1381 gtgatcacgc cgtcgccggc gatgttgtag gcgcccggcg gcgccgggga ggtcgcggcc
     1441 agcgcgatcg ccgccgcgac gtcgtcgtgg tggaccagct gcagcgggta cccggggtcg
     1501 ggcagcacag gcttgaggat cggcaccacc cgcatcgcga cccgcaccgg tgcgggcagc
     1561 cggtgccacg gcatcgtatc cgccagcacc ttcgcgtgcg gaccggcgac gatgcacggc
     1621 cgaaggatga acacctccag cgacgacccg gcagtcagct cgaccagcac ggcttcgcag
     1681 gcggccttct gcgccgagta gtagtgctcg ggtgagcccc gcgtcggcac gtcttcggtg
     1741 agcgggacgg ggttgtccgg gtgatagccg taggccgcga ccgaggacgt gaacaccagc
     1801 cggcgcggac gctcggcggc caccgtcgcc tcgaacacgt tgcgggtgcc ggccaggttg
     1861 atgcgggagc tttcctcacg tgagcccatg atgatgaacg ccaggtggac caccacgtcg
     1921 gcgtcggcga ccagcgcgtc caccgcgtcg cggtcgagga tgtcgccctg ccgatacgtc
     1981 gtcttcgtcc acccatgcgt tgacgggtca aacgggcggc gcgccatgcc gatgatccgg
     2041 tcaaccgcgg ggtcccgctc cagcgccgtg acggctgcga gcccgatttc gccggtcggt
     2101 cccgtcacgg ccacggtgac tcccatgcgt ccatcctccc ccagacgcgg agtggcctga
     2161 acccagaacg gcaaatcgtt gaacaacgcg gtgcacgggg attacaaaag agtcaagacg
     2221 cggatgcggc gagaaccatg aagctgatat cgccga
//
LOCUS       DASWBF010000391         5410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1291088,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000391 DASWBF010000000
VERSION     DASWBF010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291088"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1365
                     /locus_tag="VF874_14610"
     CDS             223..1365
                     /locus_tag="VF874_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9177249.1"
                     /translation="MLDAPSTGGGSAQRVVADLDPFDAWRTGVQREVLARVEEFVGVR
                     CAAELRGAGVDIDVDVLTQFVSGGKCLRSTFAYLGWLCRASPDDAALRATPDDAALRA
                     AASFELLHAFALLQDDVMDGSALRRGRPAAHVQFGQWHRARGLSGSATRFGESAAILL
                     ADLCLVWAAQMLRESGLPTDALQRAWPRYDAMRTELAVGQFADLVNDARGLPTLEDVL
                     DVARRKSGNYTVRRPLEIGAALSGCDNRTMVLLGSYGTAVGEAFQLRDDLLGIFGSAL
                     VTGKPSGDDLSEHKATSVVVAAHQMADPTIRRQLTELMSCDEIDDDAIARWRTLIVAT
                     GAVQWIEETIAERVTAARHQISDTRIDESVRAALASMAAVCTARTA"
     gene            1365..2840
                     /gene="crtI"
                     /locus_tag="VF874_14615"
     CDS             1365..2840
                     /gene="crtI"
                     /locus_tag="VF874_14615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX9177250.1"
                     /translation="MRSVRERADHIVVVGAGLAGLSAALHLAGRGRQVTVVERDAVPG
                     GRVGRVDSRGYRLDTGPTVLTMPDIIDDTFAAVGEDRKARLDLLPVDPAYHAVFADGS
                     GLDVHTDEARMAAAVEAFAGADAAAGYLRLRDWLTRLYRTEFDGFIAANFDSPLALLT
                     PQLARLAALGGFRRWGRVVERFINDERLCRVFTFQSLYAGVPPHRALAVYAVIAYMDT
                     VAGVYFPRGGMRALPDALAAAATGAGVKFEYSSPVTSLERSADRVTAVHTEGETRIEC
                     DAVVLTTELPQTYQLLSRAPRRPLRLRPAPSAVVAHIGCSPVKHPAHHTILFGDAWEQ
                     TFDDIIGAGRPMADPSLLVTRPSASDPSLAPPGRDLLYVLAPAPNLVRGNVDWDAVSD
                     SYAAEMLDTVRARLPGVGDDAELLHLVTPADWARQGMVAGTPFALAHTFAQTGPFRPA
                     NTVRGVANAVLAGSSTVPGVGVPTALLSGRLAADRITGVSR"
     gene            2837..3781
                     /locus_tag="VF874_14620"
     CDS             2837..3781
                     /locus_tag="VF874_14620"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX9177251.1"
                     /translation="MIRSELDAAGVSEPQLREAYRRCRELNARHGRTFFLATRLLAPA
                     QRPAVHALYGFARLADDILDDLDSPLDAEAKTARLQRLSDLFFDGGSARDDDETDLVL
                     TAVRHTAALYGIAPELFSDFLASMRMDLTVTDYPDRAALNRYMHGSAEVIGLELLPVL
                     GTIGAVEEAAPYAAALGKAFQLTNFLRDVDEDLARERVYLPADELAAFDVDRELLTWC
                     HVHRRTDPRVRSALAAQHHITRGIYRFATDGVQMLAPWSRPCVATALTLYSEILDRIE
                     ASDFAVFSQRARVGTARRLRVAGGALIQAWRSRRGSAA"
     gene            3784..4104
                     /locus_tag="VF874_14625"
     CDS             3784..4104
                     /locus_tag="VF874_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX9177252.1"
                     /translation="MEQWQYLIALGACLAITAPLELFGEGVYRQPMRTLRAVLPVALV
                     FVVWDGLAVAADVWTYNPRYATGIHVGFGIPLEELLFFIVIPLCGLLTYNAVTAMLGR
                     FRTR"
     gene            4101..4412
                     /locus_tag="VF874_14630"
     CDS             4101..4412
                     /locus_tag="VF874_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX9177253.1"
                     /translation="MTGLGYTLPAAIAAVVVVVLELTVWRTGLFRKPAYWLSMLIVVA
                     FQVLVDGWLTKLSAPIVIYDEKHTSGIRFPWDIPVEDFLFGFALVTAVLLLWGRQRER
                     R"
     gene            4399..5127
                     /locus_tag="VF874_14635"
     CDS             4399..5127
                     /locus_tag="VF874_14635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9177254.1"
                     /translation="MSVGDAGLPRRAVPGAFDVGAEAYDRMVGANPGYHAHLRMSVRR
                     MRIPDRGRGRRLLDAGCGTGASTAALLAAAPEAEIVAVDASEGMLREARGKTWPSSVR
                     FVHGRIEDLADLGVDGPFDGILAAYLVRNLADPDAQLKRFLTLLRPGATLAVHEYSVR
                     DSRAAVWMWNAVCWGVIIPSGWVNTRNTTLYRHLWRSVLAFDGAARFHRRLADASFVA
                     VHSETMPGWQRNIVHTFLADAPHD"
     gene            5120..>5410
                     /locus_tag="VF874_14640"
     CDS             5120..>5410
                     /locus_tag="VF874_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9177255.1"
                     /translation="MTSNGHLDPRRVTHPAHPGLPDAGLLASRPHAVVVGAGIAGLAA
                     ATGLAERGVSVDVVERENYLGGRVGGWTEDHDGTPIALNRGFHAFFPQYYNLR"
BASE COUNT          780 a         1841 c         1899 g          890 t
ORIGIN      
        1 agtgcgcctt tcaggtgatc ttgcagtgca agacccggcc cgcccacacg aggcgacgaa
       61 gcgtcaaccc gcacaccatt cacgagggtg cagggaccgc tgctccgcaa ggagaagctg
      121 cctgaaatgc caagtcctac aaacactcac atttcaggta acggtagaaa aggtcgatgg
      181 gttttcctcc tcccccgagg tccgagcgcg cggcgggtgg agttgctcga cgcgcccagc
      241 acgggcggcg gctcagccca gcgggtcgtc gcggacctcg acccgttcga cgcctggcgc
      301 accggcgttc agcgcgaggt gctggcccgc gtcgaggagt tcgtcggcgt caggtgcgcc
      361 gccgaactac gcggcgccgg tgtcgacatc gacgtcgacg tgctgacgca gttcgtctcc
      421 ggcggcaagt gcctgcggtc gacgttcgcg tatctcggct ggttgtgccg ggcttcacca
      481 gatgacgccg cgttgcgcgc gactcccgac gatgcggcgc tgcgcgccgc ggctagcttc
      541 gagctgctgc acgcgttcgc gctactgcag gacgacgtga tggacgggtc ggcgctgcgc
      601 cgcggacggc cggcggccca cgtccagttc ggccagtggc accgcgcccg tgggctttcc
      661 gggtcggcaa cgcggttcgg cgaatcagcc gcgatcctgc tcgccgacct gtgcctggtg
      721 tgggctgccc agatgctccg cgaaagcggc ctgcccaccg atgcgctgca gcgggcctgg
      781 ccacgttacg atgcgatgcg caccgagctc gcggtcggcc agttcgcgga tctggtgaac
      841 gacgcgcgcg gcctgcccac actggaggac gtgctcgacg tcgcgcggcg caagtccggc
      901 aactacacgg tgcgccgccc actggagatc ggcgccgcac tgtccggttg cgacaaccgg
      961 acgatggtgc tgctcggcag ttacggcacc gccgtcggag aggcgttcca gctgcgagac
     1021 gacctgctcg gcatcttcgg ttcggcgttg gtcaccggca agcccagcgg cgacgacctg
     1081 tccgagcaca aggccaccag cgtcgtcgtc gccgcccacc agatggccga ccccacgatc
     1141 cgccgtcagc tcaccgagtt gatgagctgc gacgagatcg acgacgacgc catcgcgcgc
     1201 tggcggacgc tgatcgtcgc caccggtgcg gtgcaatgga tcgaggagac gatcgccgag
     1261 cgagtcaccg cagcccgcca tcagatttcc gacacccgta tcgacgagtc ggtgcgagcg
     1321 gcgttggcca gcatggccgc cgtctgcacc gcgcggaccg catgatgcgc tctgtccggg
     1381 aacgcgctga ccacatcgtg gtggtgggcg ccggcctggc cgggctgtcc gcagcgctgc
     1441 acctggccgg gcgaggacgc caggtcacgg tcgtggagcg ggacgcggtt ccgggcgggc
     1501 gggtcggtcg cgtcgacagc cgcggctaca gattggacac cgggccgacg gtgctgacga
     1561 tgcccgacat catcgacgac accttcgcgg ccgtcggcga ggaccgcaag gcccgactgg
     1621 acctgctgcc cgtcgacccg gcttatcacg ccgtgttcgc cgacggcagc gggctcgacg
     1681 tgcacaccga cgaggcccgg atggccgccg cggtcgaggc gttcgccggc gccgacgcag
     1741 ccgccgggta cctgcggctg cgcgactggc tgactcgcct gtaccgcacg gagttcgacg
     1801 ggttcatcgc cgcgaacttc gactcaccgc tggcgctgct gaccccgcag ctggcgcggt
     1861 tggccgcact gggcggattc cggcggtggg gacgggtcgt cgagcggttc atcaacgacg
     1921 agcggctgtg ccgggtgttc acgttccagt cgctgtacgc cggcgtgccg ccgcatcggg
     1981 cgctggccgt gtacgcggtg atcgcctaca tggataccgt tgcgggcgtg tactttccgc
     2041 gcggtgggat gcgggcgttg cccgacgccc tcgcggccgc cgccaccggc gcgggcgtga
     2101 aattcgagta ctcgtcaccg gtcacctctt tggagcggtc cgctgaccgg gtgaccgccg
     2161 tacacaccga aggcgaaacc cgcatcgaat gcgacgcggt ggtgctgacc accgagttgc
     2221 cccagacgta tcagctgctg tcgcgagcac cacggcggcc gttgcggctg cggcccgcgc
     2281 cgtcggcggt ggtcgcgcac atcggctgct cgccggtgaa acaccctgcg caccatacga
     2341 tcctgttcgg agacgcgtgg gagcagacgt tcgacgacat catcggcgcc ggccgtccga
     2401 tggccgaccc gtcgctgctg gtgacccgcc cctccgccag cgacccgtcg ctcgcaccgc
     2461 cgggccgcga cctgttgtat gtccttgcgc cagcgccgaa tctggtccgc ggcaacgtcg
     2521 attgggacgc ggtcagcgac agctacgcgg ccgagatgct cgacaccgtg cgggccagac
     2581 tgcccggcgt cggcgacgac gccgagctgc tacacctggt cacgccggcc gactgggcac
     2641 gccaaggcat ggtggccggc acgccgttcg cgttggcgca cacgttcgcc cagactggtc
     2701 cgttccggcc ggccaacacc gtgcgggggg tcgccaatgc ggtactcgcg ggatcgtcga
     2761 ccgtgccggg cgtcggcgtg ccgaccgcgt tgctgtccgg gcgtctggcg gccgaccgca
     2821 tcacaggggt gagccgatga tccgttccga gcttgacgcc gctggtgttt cggagccgca
     2881 gctgcgcgag gcgtaccgcc gctgccgtga actgaacgcc cgccacggcc gcacgttctt
     2941 cctggcgacc cggctgctcg caccggccca gcggccggca gtgcacgcgc tctacgggtt
     3001 cgcccggctg gccgacgaca ttctcgacga cctcgactcc ccgctggacg ctgaggcgaa
     3061 aactgctcgg ctgcaacgac tttccgatct gttcttcgac ggcggcagtg ctcgcgacga
     3121 cgacgagacg gacctggtgc tcaccgccgt acgtcacacc gcggcgctct acggcatcgc
     3181 tcccgagttg ttcagcgact tcctggcatc gatgcggatg gacctgaccg tcaccgacta
     3241 tcccgaccgg gcggcgttga accgctacat gcacggatcg gcggaggtga tcgggctcga
     3301 gctgctaccc gtgctcggca cgatcggcgc cgtcgaagag gccgcaccgt acgcggcggc
     3361 gctgggaaaa gcgttccagc tgaccaactt tctgcgtgac gtggacgagg atctggcccg
     3421 ggagcgggtg tatctgcccg ccgacgaact tgccgccttc gacgtcgacc gcgagttgtt
     3481 gacctggtgt cacgtgcacc ggcggacaga tccccgggtg cgcagcgcgt tggccgcaca
     3541 gcaccacatc acccggggca tctatcggtt cgcgacggac ggtgtgcaga tgctggcgcc
     3601 atggtcgcgg ccgtgtgtgg ccacggcgtt gacgctgtac tcggagatcc tcgaccgcat
     3661 cgaagccagc gacttcgcgg tgttcagcca gcgcgcacga gtgggcaccg cgcgtcgcct
     3721 ccgggttgcc ggcggcgcgc tgatccaagc gtggcggtcg cggcgcggaa gtgctgcgta
     3781 gacatggagc agtggcagta tctgatcgcg ctcggcgcct gcctggcgat caccgcgccg
     3841 ctggagctgt tcggtgaggg cgtctatcgc cagccgatgc gcacgctgcg ggcggtgctg
     3901 ccggtcgcat tagtattcgt ggtctgggac gggctggccg tcgccgcgga cgtgtggacc
     3961 tacaacccgc gctacgccac cggcatccat gtcggattcg gtatcccgct ggaggaatta
     4021 ctgttcttca tcgtgatccc gttgtgcggg ctgctcacct acaacgcggt gacggccatg
     4081 ctgggccggt tcagaacgcg atgaccggct tgggttacac gctgccagcc gcgatcgcgg
     4141 cggtcgtcgt cgtcgtgctg gaactgacgg tgtggcgcac cgggctgttt cgcaagcccg
     4201 cgtactggtt gtcgatgctt atcgtggtcg ccttccaagt cctggtcgac ggctggctga
     4261 ccaaactcag cgcaccgatc gtgatctacg acgagaagca caccagcgga atccggttcc
     4321 cgtgggacat ccccgtcgag gacttcctgt tcggcttcgc actggtgacc gcggtgctgt
     4381 tgttgtgggg gcggcaacgt gagcgtcggt gacgcagggc tgccgcggcg cgccgttccg
     4441 ggcgcgttcg acgtcggcgc cgaggcgtac gaccggatgg tgggcgccaa ccccgggtat
     4501 cacgcacatc tgcgaatgtc ggttcgccgc atgcgaattc ccgatcgcgg ccgcgggagg
     4561 cggttgctcg acgcgggatg cggcaccggc gcgtcgacgg ccgcgttgct tgccgctgcg
     4621 cccgaagccg aaatcgtcgc ggtcgacgcg tccgaaggga tgctgcgtga ggcgaggggc
     4681 aagacgtggc cgtcgtcggt gcggttcgtt catggccgca tagaggacct cgccgacctc
     4741 ggcgtcgacg gcccgttcga cggcatcctg gcggcctacc tggtgcgcaa cctggccgat
     4801 ccggacgccc agttgaagcg cttcctcacg ctgctgcgcc cgggtgcgac gctggccgta
     4861 cacgagtatt cggtgcgcga ttcgcgcgcc gcagtgtgga tgtggaacgc ggtgtgctgg
     4921 ggcgtcatca tcccgtctgg ctgggtgaac acccgaaaca ccacgctgta ccggcacctg
     4981 tggcgcagcg tgctcgcgtt cgacggcgcc gctagattcc accgacggct ggccgacgcg
     5041 agtttcgttg cggtgcatag cgaaacgatg ccgggctggc agcgcaacat tgtgcacacg
     5101 ttcttggcgg acgcgcctca tgactagcaa cgggcacctc gatccccgtc gggtaaccca
     5161 cccggcccac cccggcctgc cggatgcggg cctgctggca tcgcggccgc acgcggtggt
     5221 ggtcggcgcg ggcatcgccg gtctggccgc cgccaccggg ctggcggaac gcggggtcag
     5281 cgtggacgtg gtggaacgcg agaactacct cggcggccgg gtcggcggat ggacggaaga
     5341 ccatgacggc acgccgatcg cgctgaaccg cgggttccac gcgtttttcc cgcagtatta
     5401 caacctgcga
//
LOCUS       DASWBF010000392         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_431591,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000392 DASWBF010000000
VERSION     DASWBF010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431591"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..493
                     /locus_tag="VF874_14645"
     CDS             170..493
                     /locus_tag="VF874_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177256.1"
                     /translation="MITNTPRMIDVGSDANRQRARQAGRQQAVLARKALAALQSQRPT
                     AHRDRWIRALQHRISNPDGVLAELGQTMTPPMTKHAYAALLRRALRGGGITVDDSPTT
                     AGEAL"
     gene            493..660
                     /locus_tag="VF874_14650"
     CDS             493..660
                     /locus_tag="VF874_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177257.1"
                     /translation="MIKRLISRAAVWAGRRRPGEAARAGEDPCVEREIAASLTDEALA
                     WAVGTWRKRYL"
     gene            1142..>2098
                     /locus_tag="VF874_14655"
     CDS             1142..>2098
                     /locus_tag="VF874_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015298297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177258.1"
                     /translation="MEYSDAAKVRERVETNAAQLRLRGARRHVLAAVLKLLCGWSKIT
                     DDRVGLPQIVELIAAAGGRRYDLKTIGRALAGLAADELIDYRPAQGRGTRSFIGIHDR
                     FVWDVRVLERDRSGRVITDYSCHSDPDSVTFSEPLPYKDQTHYPPTPRPETQPHSSRP
                     VAVNASTEDLREVLGHLPEPLALLPKHLRWMLGREIRQRLEAGWRPDQILDVLAAPMP
                     AHVERPWRLALWRLRHNIVGSGPRLRPLQQAWDAQTSAASRAAAEDTTARWYGVVAAV
                     TTAEQRAELLRADEVKFGRRAKDPVAALAAAGRRVARLFPEMA"
BASE COUNT          383 a          698 c          702 g          315 t
ORIGIN      
        1 gaaccacgta cttgactcaa cattcacggc tgggccgcct aagcttccta accaccagcc
       61 ccagggggtc aattttcacc tgccgccagg gggtcaaaat tcagatgccg tcgacaaccg
      121 cgctagctga gcactacggc ataccgctgc ggagctggga ggggcgtcgg tgatcaccaa
      181 cactccgcgg atgatcgacg tcgggtcgga cgccaatcgt cagcgcgcta ggcaggcggg
      241 ccgccagcag gctgtgctgg cgcggaaggc gttggccgcg ctgcaatctc agcgaccgac
      301 agcgcaccgc gacaggtgga tccgggcgct gcagcaccgc atcagcaacc ccgacggggt
      361 gttggccgaa ctcggtcaga ccatgacgcc gccgatgacc aaacacgcct acgcggcgct
      421 gctgcggcgc gcgctgcgcg ggggtggcat cactgtcgac gacagcccaa ccactgcagg
      481 ggaggccttg tgatgatcaa gcgactgatc agccgagcag cggtatgggc gggccggcgc
      541 agaccgggtg aggcagctcg ggcgggtgaa gacccgtgcg tcgaacgcga gatcgccgcg
      601 tcgctgacag atgaggccct ggcgtgggcc gtaggcacct ggcgcaagcg ctacctgtga
      661 cggctgtgcc aggtgtcgat tcctgcgaca tcaccttagg tggcgttggc accgccacgg
      721 cggggtcatg tcgatccaag tccatcggct agtggcgtgg tcggacgact cgataaagga
      781 ggtgtagcca gccactcgac gtctccgcgc aaggggacaa cccacaaatc aatagcggga
      841 tgcagaggac gtagtccccc caggtggggg atatgggaat caggtcagaa gccgaaccct
      901 cggagcgcca actccgagcc tcgcgagatc cgaagcgcca acttcgtttc tcgatccccg
      961 cgctaacagg gagaacaccg tggaaggtag ttctcagggt agtgcttacc ctggacaccg
     1021 atcaacacat ccgatcgggg tttcgccgca acaccccgtg acctgctcaa atgcagggac
     1081 ttcatgggcg tgtcgcgggg accgaaaccc cggaagaact gtttgccaga agcggcagcg
     1141 ggtggaatat tccgacgccg cgaaggtgcg cgagcgggtc gagaccaatg ccgcgcagct
     1201 gcggcttcgt ggtgcccgtc gccacgtcct ggccgccgtg ctcaagctgc tgtgcggctg
     1261 gagcaagatc accgacgacc gcgtgggcct gccccagatc gtcgaactga tcgccgcggc
     1321 cggtggacgc cgctacgacc tcaaaacgat cggtcgcgca ctggcgggcc tagcggccga
     1381 cgaactgatc gattaccggc cggcacaggg ccgcggaacg cggtccttca tcgggatcca
     1441 cgaccgtttc gtctgggacg tcagggtgct cgaacgggac cgttccgggc gggtaatcac
     1501 cgattacagc tgccactccg accccgattc cgtcaccttt tccgagccgc ttccttataa
     1561 agaccaaact cattaccccc ctaccccccg ccccgaaacg cagccgcaca gctctcgacc
     1621 ggttgcggtg aatgcctcca ccgaggattt gcgcgaggtg cttgggcacc tgcccgagcc
     1681 gttggcgctt ctcccgaagc acctgcggtg gatgctcgga cgcgagatcc gccagcgcct
     1741 ggaagccggg tggcgcccag atcagatcct cgacgtactg gcggcgccga tgcccgccca
     1801 cgtcgagcgg ccgtggcggt tggcgctgtg gcggctgcga cacaacatcg tgggctccgg
     1861 accgcggcta cgcccgctgc agcaggcctg ggacgcccag accagcgccg catcgcgggc
     1921 ggccgccgaa gacaccacgg cgcgctggta cggcgtcgtg gccgcggtga ccaccgccga
     1981 acagcgcgcc gagctgctgc gggccgacga ggtgaaattc ggacgtcgcg ccaaagatcc
     2041 cgtggccgcg cttgccgcgg cgggccggcg ggtggcccgg ctgttcccgg agatggcc
//
LOCUS       DASWBF010000393         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_431595,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000393 DASWBF010000000
VERSION     DASWBF010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431595"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VF874_14660"
     CDS             <1..1546
                     /locus_tag="VF874_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX9177259.1"
                     /translation="LVQENLCNEAFSVEHVNGADTVRQVLGDVPVSEYAQRCGVDEEL
                     LRAATRRLARADSVAVFEDLGIQQAPNSTLCSYLNKLLWILTGSFAKQGGQHLHSSFA
                     PLFSSSGVGRTPVTGAPVIAGLVPSNVVPEEILTDHPDRFRAMIVESSNPAHSVADSA
                     LVKAALESLELLVVIDVTMTETARLAHYVLPAATQFEKPEATFFNLEFPHNTFHLRHP
                     ILEPLPGTLPEPEIWARLIRELGVVDDAELQPLRRAATDGLDAFAEAFAAGVSANPMW
                     NKVLPFVLYETLGPALPDGLAGAAALWGLAQKTAFTYPDAVRRAGHADGNALFEAVLQ
                     SRSGVVFTVHEYEDDFALITHDDHKIALDMPEMLDEIRALTDARPGATTPEFPIVLSA
                     GERRAYTANDIFRDPSWRKRDADGALRVSVEDAQALGLVDGGRARISTAAGSAEASVE
                     ISEAMLAGHASLPNGFGLDFTDEDGQTRIPGVAPNALTSTEWRDAYAGTPWHKHVPAR
                     IEPVAR"
     gene            complement(1543..2358)
                     /locus_tag="VF874_14665"
     CDS             complement(1543..2358)
                     /locus_tag="VF874_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9177260.1"
                     /translation="MPTFTRDGATIHYTDIQPPPARPDTPTIFFGHGLLFSGWMFHPQ
                     IAALQNDYRCVTIDWRGQGDSAATKDGYDMDTLAADAVALIEHLGVAPVHYVGLSMGG
                     FIGQRIAARRPELIRTLSLLDTSAGPEDPDKVKRYKLLGGIYRVTGIPPLRKQVLPIM
                     FGPTFLSDPAGRPVIAEWERRLTRSRRSGVSKAVMGVANRLPIDDIDRIKAPTLVIVG
                     ADDVATPPHKSQLIAERIPGAHLEQIPACGHTSTLEQPETVTALLRNFLTQNG"
     gene            complement(2358..2732)
                     /locus_tag="VF874_14670"
     CDS             complement(2358..2732)
                     /locus_tag="VF874_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HEX9177261.1"
                     /translation="MATDVTLYLDDQPGELARVGDILGQAGANIEGLCALTGGGGQAE
                     VHILVHDPTPVFEALQRAGIKIASEEEVIVLTIEDRPGALGEVARKLGDADVNLTTAY
                     LATNTRLVLAADDLARAKAALA"
     gene            complement(2759..3169)
                     /locus_tag="VF874_14675"
     CDS             complement(2759..3169)
                     /locus_tag="VF874_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9177262.1"
                     /translation="MSAKDHPNNAPGVPMRFPPVVERLQIKYMNPVVGKVARYLPSFA
                     TVKHRGRTSGKPYETVVNAYRKGNVLAVMLGHGKTDWVKNVLAAGEADVHLFRRDVHI
                     TNPRIIPAGTDDPALPWIARRAARNMGVLAAAIA"
     gene            complement(3174..4376)
                     /locus_tag="VF874_14680"
     CDS             complement(3174..4376)
                     /locus_tag="VF874_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9177263.1"
                     /translation="MRVLISGVSIAGPVLAYWLHRYGVEVTVVERAPALRKSGGHAVD
                     LFRPAMQIVGKMGLLTAIRAKATGTDAIVIHRDNAKPVTLDLRKVYTAVSDDHVEIMR
                     DDLSEILYDATRHNVEYIFADSITSITDEGRVTFENGAPRDFDVIVGADGLHSNVRTL
                     TFGPESGFSTWIGGYLSVISVPEYLAVNGVMQVTAKIGRLAGVYTADEIDDGRAIFLF
                     RTPRPLDYHHRDVPRQKQLLREHFANMDWEVPRLLAELDETPAFYFDSITQLRLDTWS
                     RGRVTLVGDAGYCPGPAVGGSTSLAVVGAYTLAGELATHMPGYPAAFAAYEREIGDYV
                     RRSRAFAVTAAKKLVPASAFNLWALVQTARLIGLLPGSVSRTFAKFGARNLRLHDMVA
                     LKDYSPLQ"
BASE COUNT          754 a         1447 c         1524 g          781 t
ORIGIN      
        1 gctggttcag gaaaaccttt gcaacgaagc attttcggtg gagcacgtca acggcgccga
       61 caccgtcagg caggtgctgg gtgatgttcc ggtcagcgag tacgcccagc gctgcggcgt
      121 cgacgaggag ctgctgcgtg ccgcgacgcg gcggctcgcg cgggcggaca gcgtcgcggt
      181 gttcgaggat ctcggcatcc agcaggcacc caacagcacg ctgtgctcgt acctgaacaa
      241 gctgctgtgg atcctgaccg gaagcttcgc caaacagggc gggcaacacc tgcactcgtc
      301 gttcgccccg ctgttcagca gcagcggggt cggacgcacg cccgtcaccg gcgccccggt
      361 gatcgccggg ctggtgccgt ccaacgttgt gcccgaagag atcctgaccg atcacccgga
      421 ccgcttccgc gcgatgatcg tcgagagcag caatcccgct cactcggtgg cggactccgc
      481 gttggtcaag gccgcgttgg aatctttgga actgctggtg gtcatcgacg tcacgatgac
      541 cgagaccgct cggctggccc actacgttct gccggcggct acacagttcg agaagcccga
      601 agcgacgttc ttcaatctcg agttcccgca caacaccttt cacttgcgcc acccgatcct
      661 cgagccactt ccgggcacgc tgcccgagcc cgagatctgg gcccggctga tccgcgaact
      721 cggcgttgtc gacgacgccg aacttcaacc tctccgcagg gccgcgaccg acgggctcga
      781 cgcgttcgcc gaggcgttcg cggcgggcgt cagcgccaat ccgatgtgga acaaggtgct
      841 tccgttcgtg ttgtacgaga cgctgggccc ggcattgccc gacggcctcg caggtgcggc
      901 agcgttgtgg gggctcgccc agaagacggc gttcacgtat ccggacgcgg ttcgccgggc
      961 cgggcacgcc gacggcaacg cgctgttcga ggctgttctt cagagccgct ccggggtggt
     1021 gttcaccgtc cacgaatacg aagacgactt cgccctcatc acgcacgacg atcacaagat
     1081 cgccctggac atgcccgaaa tgctcgacga gatacgtgct ttgaccgatg cccgcccagg
     1141 ggcgaccacg cctgagttcc cgatcgtgct gtcggccggc gagcggcgcg cctacaccgc
     1201 caacgacatc ttccgcgacc cgtcgtggcg caaacgcgac gccgacgggg cgctgcgggt
     1261 gagcgtcgag gacgcgcagg cgctcggtct cgtcgacggg ggccgcgcgc ggatctccac
     1321 ggccgcgggt agcgccgagg caagcgtcga aatcagcgaa gccatgctgg ccgggcacgc
     1381 gtcgctgccc aacgggttcg gtctcgactt caccgacgag gacggccaga cccggatccc
     1441 cggtgtcgcg cccaacgcgc tcacgtccac cgaatggcgc gacgcatacg cgggcacgcc
     1501 gtggcacaaa cacgtccccg ccaggatcga acccgtagct cgctagccgt tctgcgtcaa
     1561 gaagtttcgc agcagcgcgg tgaccgtctc gggctgctcg agcgtgctgg tgtgcccgca
     1621 ggcggggatc tgctcgaggt gcgcaccggg aatccgctcc gcgatgagtt gcgacttgtg
     1681 aggcggggtc gccacgtcgt cggcgccgac gatgacgagc gtcggcgcct tgatgcggtc
     1741 aatgtcgtcg atcgggagtc ggttggcgac gcccatgacg gccttgctga cgccggagcg
     1801 gcgactgcgc gtcagccgtc gttcccactc ggcgatgacg gggcgtccgg ccggatcaga
     1861 cagaaacgtg ggtccgaaca tgatgggcag cacctgtttt cgcagcggcg ggatgcccgt
     1921 gacccggtag atgccgccaa gcagcttgta ccgcttgacc ttgtcgggat cttcggggcc
     1981 ggcgctggtg tcgaggaggc tgagggtgcg gataagttcc ggacgcctgg cggcgatccg
     2041 ctgaccgatg aagcctccca tggacaggcc gacatagtgc acgggcgcga cgccaagatg
     2101 ctcgataagg gcgacggcgt cggcggccag cgtgtccatg tcgtagccgt ccttggtcgc
     2161 ggcgctgtcg ccctgcccgc gccaatcgat tgtgacgcaa cggtagtcgt tctgcagcgc
     2221 ggcgatttgc ggatgaaaca tccagccgct gaacaacagt ccgtgtccga agaagatcgt
     2281 cggcgtatcg ggccgggcgg gcggcggctg aatgtccgtg taatgaatgg tcgcgccgtc
     2341 gcgggtgaac gtgggcatca ggcgagcgcg gccttggctc gagcgaggtc atccgccgcc
     2401 agcaccagcc gggtgttggt cgcaaggtac gcggtggtga ggttgacatc ggcatcgccg
     2461 agcttgcggg cgacctcacc gagtgcgccg ggccggtcct cgatggtcag gacgatcacc
     2521 tcctcctcgg aggcgatctt gattccggcg cgttgcaacg cctcgaacac gggcgtcgga
     2581 tcgtgcacga gaatgtgaac ctccgcctgg ccaccgccgc cggtcagggc gcacaagccc
     2641 tcgatgttgg cgccggcctg accgaggatg tcgccgacgc gagccaattc gccgggctgg
     2701 tcgtcgagat agagcgtcac atcggtcgcc atgacgcgct ccttccgtcc gctggtcgtt
     2761 aggcgattgc ggcagctaac acgcccatgt tgcgcgccgc cctccgtgcg atccacggca
     2821 gcgccgggtc atcggttccc gcgggaatga tccgcgggtt ggtgatgtgc acgtcgcgac
     2881 gaaacaggtg cacatcggcc tctccggcgg ccagcacgtt cttcacccag tccgtcttgc
     2941 cgtggccgag catgacagcg agcacgttgc ccttgcggta ggcgttgacg acggtctcgt
     3001 aaggcttgcc cgacgtccgg ccgcggtgct tgacggtcgc gaaactcggc agatagcgcg
     3061 cgaccttgcc gaccaccggg ttcatgtact tgatctgcag gcgttcgacg acgggcggaa
     3121 acctcatcgg cacgcccggg gcgttgttgg gatgatcctt cgcggacatg cggtcactgt
     3181 aacggcgagt aatccttcag ggcgaccatg tcgtgcaagc gaaggttcct ggcgccgaat
     3241 ttggcgaagg ttcggctgac actgcccggc agcaagccga tcagccgggc ggtttgtacc
     3301 aaggcccaca gattgaatgc gctggccggc acgagcttct tggcggccgt caccgcgaag
     3361 gcgcggctac ggcgcacata gtcaccgatc tcgcgctcat aggcagcgaa cgcggcaggg
     3421 taacccggca tgtgggtggc cagctcacct gccaaggtgt acgcgccgac gacggccagg
     3481 ctggtgctgc cgcccacggc ggggcctggg caatagcccg cgtcaccgac caacgtgacc
     3541 cgaccccgcg accaggtatc gagccgcaac tgcgtgatcg agtcgaagta gaaggcgggg
     3601 gtctcgtcga gttcggccag cagtcgcggc acctcccagt ccatgttggc gaaatgctca
     3661 cgtagcaact gcttctgccg cggcacatct cggtggtggt agtccaacgg cctcggggtg
     3721 cgaaacagga agatggcgcg gccgtcgtcg atctcgtcag cggtgtagac accggccaat
     3781 cgccctatct tggcggtgac ttgcatgacg ccgttgacgg ccaggtattc gggcaccgag
     3841 atcaccgaca gatagccgcc gatccacgtc gaaaagcctg actctggacc gaaagtcagc
     3901 gttcggacat tggagtgcag cccgtcggcg ccgacgatca cgtcgaagtc acgcggcgcg
     3961 ccgttctcga aggtgacgcg gccctcatcg gtgatcgagg tgatggagtc ggcgaagatg
     4021 tattcaacat tgtgacgggt tgcgtcgtag aggatctcgc tcaggtcgtc gcgcatgatc
     4081 tccacgtgat cgtcggagac ggccgtgtac accttgcgca ggtcgagcgt gaccggtttc
     4141 gcgttgtcac ggtggatgac gattgcgtcg gtgcccgtcg ctttggcgcg gatcgccgtc
     4201 agcagaccca tttttccgac gatctgcatg gcgggccgga aaaggtcgac cgcgtggccg
     4261 cctgacttcc gtaacgcggg tgcccgctcg acgacagtga cctcgacgcc atacctgtgc
     4321 agccagtacg ccagtacggg cccggcgatg ctgacgcctg agatcaggac tctcatgagc
     4381 acctccagcg gttgccgtgt ggcatgcgga tgtggcgttg ctgaccgtga cgaggcacgc
     4441 ccgcggctgt ccgccgagcg ccgacgttat ggctgatgca agtagttaat catgtggctc
     4501 tttcgg
//
LOCUS       DASWBF010000394         7754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1154238,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000394 DASWBF010000000
VERSION     DASWBF010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154238"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VF874_14685"
     CDS             complement(<1..514)
                     /locus_tag="VF874_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9177264.1"
                     /translation="MSAHNSLALTGERTVPGVAEENYWFRRHEVVYRRLASHCAGHDV
                     LEAGCGEGYGADLIADVARRVIALDYDELTVAHVRARYPRVEVRHGNLAALPLPDGSV
                     DVVVNFQVIEHLWDQPQFVAECMRVLRPGGLLLMSTPNRITFTPGSDTPVNPFHTREL
                     NASELTELLAG"
     gene            722..1522
                     /locus_tag="VF874_14690"
     CDS             722..1522
                     /locus_tag="VF874_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX9177265.1"
                     /translation="MTNIVVLIKQVPDTWSERKLKDDDYTLDRDAADAVLDEINERAV
                     EEALLIKEKQAADGTESTVTVLTAGPERATEAIRKALSMGADKAVHVLDDGMHGSDIV
                     QTGWVLARALGTIEGTELVIAGNEATDGVGGAVPAIIAEYLGLPQLTHLRKLSVEGGK
                     ITGERGTDDGLFTLEASLPAVVSVTEKINEPRFPSFKGIMAAKKKEVTTLTLAEIGVE
                     GDEVGLANAGSKVLASNPKPPKSAGEKVEDDGEGGKKIAQYLVSQKII"
     gene            1541..2497
                     /locus_tag="VF874_14695"
     CDS             1541..2497
                     /locus_tag="VF874_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX9177266.1"
                     /translation="MAEVLVLIEHAEGAPKKVSTELLTAAKALGEPSAVVVGAPGTAE
                     PLAGALKAAGAEKIYVAEADFVDDYLLLPQVDVLASLVESASPAAVLLAASADGKEVA
                     GRLAARIGSGVLVDVVDVQEGNKGVHSIFGGAYTVEAQVDGDVAVMTVRPGAIEAEAS
                     EGAGEQVTVEVPQPAENATKITAREPAAAGDRPELTEATIVVAGGRGVGSADNFSVVE
                     ELADSLGAAVGASRAAVDSGYYPGQFQVGQTGKTVSPQLYIALGISGAIQHRAGMQTS
                     KTIVAVNKDEEAPIFEISDYGIVGDLFKVAPQLTEAVKARKG"
     gene            2691..3491
                     /locus_tag="VF874_14700"
     CDS             2691..3491
                     /locus_tag="VF874_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9177267.1"
                     /translation="MTHAWLPRASCDESCVRAESPRVWRATFRCLLIAVLLPVLPLLA
                     IPHPGRARAVRWFCWVVLRTMGVRIAKSGGPIRNVPGVLVVSPHMSWVDVLVINALIP
                     GTFVAKAEVTRGPALGVLARVMNVIPIDRARLRRLPDVVDAVATRLRSGQTVVTFPEG
                     TTWCGLAHGPFRPAMFQAAIDAGRPVQPLRLSYHHRDGLPSTVPALVGNDTLPASFLR
                     VLTAPRTVAHVRVEALQLPGTHRRDLAMRCQEAVRGARESHHERALVA"
     gene            3538..4722
                     /locus_tag="VF874_14705"
     CDS             3538..4722
                     /locus_tag="VF874_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX9177268.1"
                     /translation="MARPVYLDHAATTPMHPDAIEAMAAALATVGNASSLHTSGRAAR
                     RTIEESREALADKLGARPSEVIFTAGGTESDNLGVKGIYWARRDADPRRRRIVTSAVE
                     HHAVLDAVEWLAEHEGAEVTWLPTEADGSVSPAALRDTLQRHDDVALVSVMWANNEVG
                     TIMSVTELAAVASEFDVPIHSDAVQAIGQLPVSFAESGLSAMSIAAHKFGGPTGVGAL
                     LLRRDTACVPLLHGGGQERDVRSGTPDVAGAVGMAVAARLSVDSLETHTEKLTALRDR
                     LIDGVLGTVDDVALNGPRHARLPGNAHFTFTGCEGDALLMLLDANGIECSTGSACTAG
                     VPQPSHVLLAMGAGSAAARGSIRLSLGRTSVADDVDTVLDVLPAAVSRARQAALASAG
                     AK"
     gene            4719..5783
                     /gene="mnmA"
                     /locus_tag="VF874_14710"
     CDS             4719..5783
                     /gene="mnmA"
                     /locus_tag="VF874_14710"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX9177269.1"
                     /translation="MRVLAAMSGGVDSSVAAARMVDAGHDVVGVHLALSSAPGTLRTG
                     SRGCCSKEDAGDARRVADKLGIPFYVWDFADRFKQDVIDDFVSSYARGETPNPCVRCN
                     EKIKFSALAARALALGFDAVATGHYARLSGGRLRRAVDSDKDQSYVLAVLTADQLRHA
                     LFPVGDTPKPQIRAEAAAHGLAVADKPDSHDICFIPSGDTRAFLGSRIGVRHGAVVDR
                     AGTVLAQHDGVHGFTIGQRKGLGIAGPGPDGRPRYVTAIDASTATVTVGDAGDLDVRD
                     VTGERPVFTSGGAPVGPVECEVQVRAHGGAVDAVAELVGDRLEARLRTPVRGVAPGQT
                     MVLYRPDADGDEVIGSATII"
     gene            complement(5776..6417)
                     /locus_tag="VF874_14715"
     CDS             complement(5776..6417)
                     /locus_tag="VF874_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEX9177270.1"
                     /translation="MARRSSALLSCLAAVTLASGCTDVVGGVASMPPTEGPYLDGMDV
                     DELLLTTPEMRDLTGAGLDLNGVPGMDSKYTVDDELLVESAPPECQFVFRESSVFGPD
                     VKQFHKTSFQTPPKSALLSEAAAAYVDTNTARRAFDNVTNLVKACGDTPSGYAYVYDW
                     TAEPEILRAESLGDCGRIYRLEDTVLIEVTYCGYSHVVPDLVATRIAARVGAK"
     gene            6462..7490
                     /locus_tag="VF874_14720"
     CDS             6462..7490
                     /locus_tag="VF874_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9177271.1"
                     /translation="MSAFAAATGIGSWPGRSPRQAAEVVVGELHSLPHLVELPGRGVG
                     ADMIGRAGALLVDIAIDTTPRGYRIAARPGAVSRRARSFLDEDIDALEEAWERAGLRG
                     GDRTVKVQAPGPVTLAAQLELVGGHRVITDPGALRDVSSSLAEGVALHRAEVARRLGS
                     PVVVQFDEPSLVAALAGRLTGVTALTPVHPVDETVTVDLLDECVAAVGGEVAVHCCAA
                     DVPWESLCRSMIHAVGIDSSALDQTSLDGLGEFVDSGRTVMLGIVPLTAPTQPPSVDQ
                     LIERAVTLTDRIGFPRRFLAERVGVTPACGLAGATSEWARIAIELAQKTVDALSADPE
                     VISGRRAP"
     gene            complement(7468..>7754)
                     /locus_tag="VF874_14725"
     CDS             complement(7468..>7754)
                     /locus_tag="VF874_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX9177272.1"
                     /translation="PRGTPGHCRAVRRGNANAGLRPVPARLDTRLPGLMGLDAGHVGR
                     FYYPAYMGPSGWLGLDLTVGDVEWDEVRELVDASYRLVAPRKLIKELDGR"
BASE COUNT         1222 a         2472 c         2846 g         1214 t
ORIGIN      
        1 cgccggccag caactccgtc agctcagaag cgttgagctc ccgcgtgtgg aacgggttga
       61 ccggagtgtc gctgccgggg gtgaacgtga tcctgttcgg cgtcgacatc agcagcaggc
      121 cgcccggccg caacacccgc atgcactcgg cgacgaactg cggctggtcc cacagatgct
      181 cgatgacctg gaaattcacg accacgtcca ccgaaccgtc cggcagcggc agggcggcga
      241 ggttgccgtg cctgacctcg acccgcggat agcgtgcgcg cacgtgggcg accgtgagtt
      301 cgtcataatc cagcgcgatc acgcgccgcg ccacgtcggc gatcaggtcg gcaccatacc
      361 cctccccgca accggcctcc agcacgtcgt gcccggcgca gtgcgacgcc agccgccggt
      421 agaccacctc gtggcggcgg aaccagtagt tctcctcggc gacgccgggg accgtgcgct
      481 cgccggtcaa cgccagggag ttgtgtgcgc tcatcgcaac gcaggctaac tcggatcggc
      541 cgcttggcga gaggtgggga gcaccgcgca gtcagacgac ctgcgacgac cggttatggt
      601 gtacgggcga accacgcgaa gttacccgtc agtaacatgg cgtgcgggcg attccgagat
      661 tgctggccgg ttgtcgctgg ccggcagaca tccaggtacg cgaggagggc gaaccagacc
      721 catgacgaac atcgtggtcc tgatcaaaca ggtcccagac acgtggtcgg agcgcaagct
      781 caaggacgac gactacacgc tggaccgcga cgccgccgac gctgtgctcg acgagatcaa
      841 cgagcgcgcc gtcgaagagg cgctgctgat caaggagaaa caggccgccg acgggaccga
      901 gagcaccgtc accgtgctga ccgccggccc ggagcgcgcg accgaggcca tccgcaaggc
      961 gctgtcgatg ggcgccgaca aagcggtgca cgtgctcgac gacggcatgc acggctccga
     1021 catcgtgcag accggctggg tgctcgccag ggcgctcggc acgatcgagg gcaccgagct
     1081 cgtgatcgcc ggcaacgagg ccaccgacgg tgtcggcggt gcggtgccgg cgatcatcgc
     1141 cgagtacctg ggcctgccgc agctcaccca tctgcgcaag ctctcggtcg agggcggcaa
     1201 gatcaccggc gagcgcggaa ctgacgacgg cctgttcacg ctcgaggcgt cgctgccggc
     1261 cgtggtgagc gtcaccgaga agatcaacga gccgcgcttc ccgtcgttca agggcatcat
     1321 ggccgccaag aagaaggaag tgaccacgct gacgctggcc gagatcggcg tcgagggcga
     1381 cgaggtcggg ctggccaacg ccggctcgaa ggtgttggcg tcaaacccca agccccccaa
     1441 gagcgcaggc gagaaggtcg aggacgacgg cgagggcggc aagaagatcg cccagtacct
     1501 ggtttcgcag aagatcatct gagagaaggc ttgactactg atggctgaag tacttgttct
     1561 tatcgagcac gccgagggag cgcccaagaa ggtgagcacc gaattgctca ccgccgcaaa
     1621 ggcattgggt gagccgtcgg cggtcgtcgt cggcgcgccg gggaccgccg agccgctggc
     1681 cggcgcgttg aaggctgcgg gcgcggagaa gatctacgtc gccgaggccg acttcgtcga
     1741 cgactacctg ctgctgccgc aggtggatgt gcttgcgtcg ctggtcgagt cggccagtcc
     1801 ggcggctgtc ttgctggccg ccagcgctga cggcaaggag gtcgcgggcc ggctggcggc
     1861 gcgaatcggc agcggcgtgt tggtggacgt ggtcgacgtc caagagggca acaagggcgt
     1921 gcactcgatc ttcggtggcg cctacaccgt cgaggcgcag gtcgacggcg acgtcgcggt
     1981 aatgacggtg cggcccggcg cgatcgaggc ggaggcgtcg gagggcgcgg gcgagcaggt
     2041 gacggtcgag gttccgcagc cagccgagaa tgcgaccaag atcacagcac gtgagccggc
     2101 ggcggccggc gaccgcccag agctcaccga ggccaccatc gtggtggccg gtggccgcgg
     2161 cgtcggcagc gcagacaact tcagcgtggt cgaggaactc gccgactcgc tgggtgcggc
     2221 ggtcggtgcg tcgcgcgccg cagtggattc cggctactac ccggggcagt tccaggtggg
     2281 tcagaccggc aagaccgtgt cgccgcagct ctacatcgcg ctgggtatct ccggggcgat
     2341 ccagcaccgc gcgggcatgc agacgagcaa gacgatcgtc gccgtcaaca aggacgaaga
     2401 ggcgccgatc ttcgagatct ccgactacgg catcgtgggc gacctgttca aggtcgcccc
     2461 gcagctgacc gaggcggtca aggcccgcaa gggctagctc cgcgcacagg cccccgcaca
     2521 gcgcgcgtgt cgggggcttt gcgtcttctc gcgaggtagg tgagttgtca cctgacgtgc
     2581 acggacacgt cacgcacacg atgccgtcgc gagatgcggg tcagcgacgg tgcagccgtg
     2641 agcactccac acacgcgcta cctcaagcgg ctgcgatcgg tggctgggcg atgacgcatg
     2701 cctggctgcc gcgtgcatca tgcgacgaaa gctgtgtgcg tgccgagtcg ccgcgtgtgt
     2761 ggcgcgcgac gtttcggtgc ctgctgatcg cagtgctact gccggtgctg ccgctcttgg
     2821 ccatcccgca tccgggccgg gcgcgggctg tgcgatggtt ctgctgggtc gtcctgcgca
     2881 ccatgggcgt gcgaatcgcc aagtccggcg gcccaattcg caacgtgccg ggtgtgctgg
     2941 tggttagccc gcacatgtcg tgggtcgacg tactggtgat taatgcgctg ataccgggca
     3001 cgttcgtcgc gaaggccgaa gtcaccaggg ggccagcgct gggcgtgctc gcgcgtgtga
     3061 tgaacgtcat tccgatcgac cgtgcccggt tgcggaggtt gcccgacgtc gtcgatgccg
     3121 tggcaacccg gctgcgatcg ggccagaccg tcgtcacatt cccggagggc acgacatggt
     3181 gcgggctggc acatggcccg tttcgccccg cgatgtttca ggcggcgatc gacgcaggcc
     3241 gcccggtgca gccgctgcgg ctgagctatc accaccgcga cgggctcccg tcgacggtcc
     3301 ccgcgttggt tggcaacgac accctgcccg cgtcattcct ccgcgtgctg acggcgcccc
     3361 ggacggtcgc acacgtgcgc gtcgaggctc tgcaactgcc cggcacgcac cgtcgcgacc
     3421 tggccatgcg ctgccaagag gccgtccgcg gcgcccgcga gtcacaccac gagcgcgcgc
     3481 tggtcgcctg agcggccggc cggggcctga gcctgcgaca gctaagctgg acgggctatg
     3541 gcccgtccgg tgtatctcga ccacgccgct accaccccga tgcatccaga tgcgatcgag
     3601 gcgatggcgg ctgcgctggc aaccgtcggc aatgcctcgt cgttgcacac atccggtcgc
     3661 gccgcccgcc gcacgatcga ggagtcgcgg gaggccttgg ctgacaagct cggtgcgcga
     3721 ccgtcggagg tgatattcac cgcgggtggc accgaaagcg acaaccttgg cgtcaagggc
     3781 atctactggg cccgtcgcga cgccgacccg cgcaggcgcc ggatcgtcac cagcgccgtc
     3841 gagcatcacg ccgtgctcga cgctgtcgag tggttggccg agcacgaggg tgccgaggtg
     3901 acgtggctgc cgacagaggc cgacggctcg gtttcgccgg ccgcgctgcg agatacgctg
     3961 cagcgacacg acgacgtcgc gctggtgtcg gtgatgtggg caaacaatga ggtcggcacg
     4021 atcatgtcgg tcaccgaatt agcagccgtc gcatccgaat tcgatgtgcc tatccacagc
     4081 gacgcagtcc aggcgattgg gcagctgccg gtttcgttcg ccgagagcgg gctttcggcg
     4141 atgagtatcg ccgcgcataa gttcggcggg ccaaccggtg tcggcgccct gctgctgcgt
     4201 cgcgacaccg cctgcgtacc gctgctgcac gggggcggcc aggagcgcga cgtgcgctct
     4261 ggcacaccag atgtcgccgg tgcggtcggt atggctgtgg cggccaggct ttcggtggac
     4321 agtctcgaaa cgcataccga gaagctgacc gcgttgcgcg atcgcttgat cgacggcgtg
     4381 ttgggcacgg tcgacgacgt ggcactgaac ggtccgcgcc acgcccggct gccgggcaac
     4441 gcgcacttca ccttcaccgg gtgcgagggt gatgcgttgc tgatgttgtt ggacgccaac
     4501 gggattgagt gttcgacggg ttctgcctgc accgcgggcg tcccgcagcc atcccatgtg
     4561 ctgctggcga tgggcgcggg ttctgcggcc gcccgaggct cgatccgact ctcgttgggc
     4621 cgcaccagcg ttgctgacga cgtcgacacc gtgctcgacg tgctgcccgc cgcggtttct
     4681 cgcgcacgcc aggcggcgct ggccagcgcg ggggcgaagt gagagttctc gccgcgatga
     4741 gcggcggcgt ggattcgtcg gtggccgccg cacggatggt cgatgccggc cacgacgtgg
     4801 taggcgtgca cctggcgttg tcgtcggccc cgggcacgct gcgcaccgga tcacgcggct
     4861 gctgctccaa ggaagatgcg ggtgacgccc gacgcgtcgc cgacaaactc ggaatcccgt
     4921 tctacgtctg ggatttcgcc gaccgattca aacaagacgt catcgacgac ttcgtgtcgt
     4981 cctatgcccg cggtgagacc ccgaatccgt gcgtgcggtg caacgagaag atcaagttct
     5041 cggcactggc cgcgcgggcg ttggcgctgg gcttcgacgc ggtggccacc gggcactacg
     5101 cgcggttatc cgggggacgg ctgcgacggg ctgtcgactc cgacaaagac cagtcctacg
     5161 tgctggcggt gttgaccgcg gaccagttgc ggcacgcgct gtttccggtg ggagacacgc
     5221 cgaaaccgca gattcgggcc gaggcggccg cccacgggct cgccgtcgcg gacaagcccg
     5281 acagccacga catctgcttc atcccgtccg gcgacacacg ggccttcctg ggctcgcgca
     5341 tcggggtgcg ccacggcgcc gtggtggaca gggccggaac cgttctggca cagcatgacg
     5401 gcgtgcacgg cttcacgatc ggccagcgca agggtctcgg catcgccgga ccggggccgg
     5461 atgggcgccc gcggtacgtc accgcgatcg acgcctcgac cgccacggtg acggtcggcg
     5521 acgccggcga tctcgatgtg cgggacgtga cgggggagcg gccggtgttc acgtcgggtg
     5581 gcgcgcccgt cggaccggtc gagtgcgagg tgcaggtgcg cgcgcacggc ggcgcggtgg
     5641 acgcggtggc cgaactcgtg ggggatcggt tggaggcccg gctgcgtacg ccggtgcgcg
     5701 gtgtggcgcc gggacagacg atggtgctct accggccgga cgccgacggc gacgaagtga
     5761 tcggcagcgc gacgatcatt tagcgccgac gcgtgcggcg atgcgcgtgg cgacgaggtc
     5821 gggcacgaca tggctgtagc cgcagtaggt cacctcgatg agcacggtgt cctcgagtcg
     5881 gtagatgcgg ccgcagtcac caaggctttc cgcgcgcagg atttcgggct cggccgtcca
     5941 gtcgtagacg tacgcgtagc ctgacggagt gtcaccgcac gccttcacga ggttggtgac
     6001 gttgtcgaac gcgcggcgtg cggtgttggt gtcgacgtag gcggccgcgg cctccgatag
     6061 cagcgcggac ttgggcggtg tctggaagct ggtcttgtgg aactgcttga cgtcggggcc
     6121 gaagaccgaa ctttcgcgga agacgaattg acattcagga ggggcggatt cgaccagcag
     6181 ctcgtcgtcg acggtgtact tcgagtccat gccgggcacg ccgttgaggt cgagtcccgc
     6241 gccggtgagg tcgcgcattt ccggggtggt gagcagcagc tcgtccacgt ccatgccgtc
     6301 gaggtaggga ccttcggtcg gcggcataga agcgacgccg ccgacgacgt ccgtgcatcc
     6361 gctggcgagc gtcaccgcgg cgaggcagct gagcagcgcg ctgctgcgtc gggccatgat
     6421 gtggccagac taacgcgcac ctgtccaata ctgttgggca agtgagtgct ttcgccgccg
     6481 cgacgggcat cggctcgtgg ccgggtaggt cgccgcggca ggccgcagaa gtggtcgtag
     6541 gcgaactgca ctctctgccg catctggtcg agttgccggg ccgcggtgtg ggggcggaca
     6601 tgatcggcag ggccggcgcg ctgctggtcg acatcgcgat cgacacgaca ccgaggggat
     6661 accggatcgc ggcgcggccc ggcgcggtga gccggcgggc gcgcagcttc ctcgacgagg
     6721 acatcgacgc gctggaggag gcgtgggagc gggccgggct gcgcggcggc gaccgcaccg
     6781 tcaaggtgca ggcgccaggg ccggtgacgc tggcggcgca gctggaattg gtgggcggcc
     6841 atcgggtgat caccgacccg ggggcgctgc gggacgtgtc gtcgtcgttg gcggaggggg
     6901 tcgcgttgca ccgcgcggaa gtcgccagac ggttgggcag cccggtggtg gtgcagttcg
     6961 acgagccgtc gttggtggcg gcgctggcgg gccggctgac cggagtgacg gcgttgacgc
     7021 cggtgcaccc cgtggacgaa acagtgacgg tggatttgct cgacgagtgc gtggctgccg
     7081 taggcggcga ggtggcggtg cactgttgtg ccgctgatgt gccgtgggaa tcattgtgcc
     7141 gcagtatgat tcatgcggtt gggatcgact cgtccgcgct ggaccagact tcgctcgacg
     7201 gcctcggcga gttcgtcgac agcggtcgga cggtgatgct gggcatcgtg ccgttgacgg
     7261 cgcccacgca accgccgtcc gttgaccagc tgatcgaaag agctgtaacg ctcaccgacc
     7321 ggatcggctt cccgcgtcgt ttcctcgccg agcgcgtcgg cgtcacgccg gcgtgcggcc
     7381 tggccggtgc gacatccgag tgggcccgca tcgcaatcga attggcgcag aagacggttg
     7441 atgcgctaag cgcagacccg gaggtgatta gcggccgtcg agctccttga tcagcttgcg
     7501 tggggccact aatcggtagg acgcgtcgac cagctcgcgg acctcatccc actccacgtc
     7561 gccaacagtg aggtcgaggc cgagccagcc ggacgggccc atgtaggcgg ggtagtagaa
     7621 ccgaccgaca tggcccgcgt ctaagcccat taggcctggt aggcgggtat ctaggcgggc
     7681 cggtacgggc ctgagcccgg cgttggcgtt accccggcgt accgcgcggc agtgccccgg
     7741 cgtaccgcgc ggca
//
LOCUS       DASWBF010000395         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_770021,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000395 DASWBF010000000
VERSION     DASWBF010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770021"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..660)
                     /locus_tag="VF874_14730"
     CDS             complement(235..660)
                     /locus_tag="VF874_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX9177273.1"
                     /translation="MKIVDGDTVDVRDDVRGRLRIRLLGIDTPEVHRPHYSVGCLGPE
                     ATEFAQSTLQGRRVAIITDAGQGQFDRYGRTLAYLDKADGWDYSIEAARAGFAHAYIY
                     ENHPAERYPQIAAAEQEARSAGRGLWGPPCYGHTESVPT"
     gene            complement(877..1080)
                     /locus_tag="VF874_14735"
     CDS             complement(877..1080)
                     /locus_tag="VF874_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177274.1"
                     /translation="MRSTSTPADRKVRDILVRIIETMHDESQRELARDLLLTLPAVRD
                     WPHDTLRAAYEPFTFAPPRQELE"
     gene            1130..2119
                     /gene="ligD"
                     /locus_tag="VF874_14740"
     CDS             1130..2119
                     /gene="ligD"
                     /locus_tag="VF874_14740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX9177275.1"
                     /translation="MTSQRAERTPVPPAPMLATAGPIPAGDTWAFEPKWDGARAALRV
                     DGDRTELFSRNANNLSSSFPELREACGAALGRRRAVLDGEVIVLDSLARPSFSLLQRR
                     LHVSRPPSSLRTRLAATLVIFDVLHLDGEDLTPLPYQDRRAALEDLALGGSNGGSRVV
                     TSPAWTNIEGSAVFEVIEQMGLEGVVAKATMSPYQAGRRSRLWVKTPVRRSNLFAVGG
                     WIPSSSRADAVGSLLVGGYNVAGDLVYCGHVSSGFSDRMRRALYVQLSEIRSPASPFR
                     SSHPDPDTGRVRWVKPVLVGRVEYREFTGRLRHAAWKGAQAVDPRLAVVPPLL"
     gene            complement(2142..2336)
                     /locus_tag="VF874_14745"
     CDS             complement(2142..2336)
                     /locus_tag="VF874_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177276.1"
                     /translation="MARDELGASYRAGLRAGGDDVDWLTWYRDHINRWPAGTMKDIVT
                     ARLDSPDYQQHMQTLPDYWR"
     gene            complement(2404..2841)
                     /locus_tag="VF874_14750"
     CDS             complement(2404..2841)
                     /locus_tag="VF874_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177277.1"
                     /translation="MAFDYAARDGGHFSRAAERHYRVLRTQALRQILALARNGALKPD
                     WRDTLDTPQRSATAHHIYTDGDLLAIEDLRQMDYAAWEPGHASSWRDALSAWYSAARQ
                     ALHEDRALALQLQVTSTQDRDKTLASALRLRSQGVNAVDKCCD"
     gene            3664..4575
                     /locus_tag="VF874_14755"
     CDS             3664..4575
                     /locus_tag="VF874_14755"
                     /inference="COORDINATES: protein motif:HMM:NF022388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2637 domain-containing protein"
                     /protein_id="HEX9177278.1"
                     /translation="MTSAAEERRAVRFFWVVLMLASSASVAGNVTHAVWNASGPAMVV
                     AALAALVAPLSLLGATHSVGVLARVRTNSFTFWASMAITIAVVVCAFWLSFHALRSVA
                     VNLAQVDPGIAWMMPVCIDLSIAGSTLALLSLSRPSAAAPTASQTARAPRASTNGSTS
                     PASAATNGRAPAATSGVQDVVTRPRPVGGPHNNGHGRAGVALEERPTLVAVGQMVEAV
                     TAAEMHPEAEARWSGVAETLVREKRTKIPARVVAKVLALREAQTPPSTIGRLLNVHHS
                     AVRRILDGADDVDRSGSGPGDVPGMAQ"
BASE COUNT          716 a         1482 c         1705 g          872 t
ORIGIN      
        1 cggcagatgc tgagcggggt cgggcggcgg gcgtgagtat ccgctgcggc tcagatcgtg
       61 cgccgcgact accgatggga aatgcagcga tcttgagaat cagctgcgtg cacagtgggc
      121 gagacaacca aacatcgcag ctggtcaggg cgccaggaag agcagcggag gttgagaacc
      181 taatgctggt gcttgctgcg cggccgtgta ttggtcagag cgggcactgg tcgtctatgt
      241 cggcaccgat tcggtgtgtc cgtagcacgg tgggccccat agcccgcgcc cggcggatcg
      301 tgcttcttgt tctgcggctg cgatctgggg gtaacgctcg gcggggtggt tctcgtagat
      361 gtaggcgtgg gcaaagccgg cgcgggcggc ttctatggag tagtcccacc cgtccgcctt
      421 atccaagtag gccaaagtgc gtccgtaccg gtcgaattgg ccttgcccgg cgtcggtgat
      481 gatggcgacg cgccgccctt gcagggtgga ttgggcgaac tcggtggcct cgggccccaa
      541 acagccgaca ctgtaatgcg ggcgatggac ctcgggcgtg tcgatgccca acagccgaat
      601 gcgcagccgg ccgcgcacgt cgtcgcgcac gtcgacggtg tcgccgtcaa cgatcttcac
      661 gaccacggcg gtcgtgggaa ttggatcaga gtgtgttgcc ggcgcgcatg agacggctag
      721 aaggcagact gctgagcagc tggccgcggc gaggcgggat ggcatggatc tatcgtgccc
      781 gtagggccga caatgggggc tggacacacc tgcggtcggc gggcgggccc acgacgaacc
      841 tccacgaagg gctcccccgc ctgccactgt tgaggactac tccagttcct ggcggggcgg
      901 cgcgaacgtg aacggctcgt aggccgcacg cagagtgtcg tggggccagt cccgcacagc
      961 cggcaacgtc agcaggaggt cgcgtgcgag ttcgcgctgg ctctcatcgt gcatggtctc
     1021 gataatccgg acgagaatgt cgcggacctt gcggtcggcg ggggtcgacg tcgacctcat
     1081 ctggcactgt tcgactgtac gacttgtccg ccgcgggcag actggtctga tgacctcgca
     1141 gcgggcagaa cgaacaccgg tgccgccggc tccaatgctt gccactgcag ggccgattcc
     1201 cgccggcgac acatgggcgt tcgagcccaa gtgggacggc gcgcgggcgg cattgcgggt
     1261 cgacggggat cggaccgagc tgtttagccg caacgccaac aatctgtcgt cgtcgttccc
     1321 ggagctgcgc gaggcgtgtg gtgccgcgct tggccgccgg cgcgccgtcc tcgacggcga
     1381 ggtgatcgtc ctggactccc ttgcgcgccc gagcttttca ctgcttcagc ggcgcttgca
     1441 cgtgagcagg ccaccgagtt cgctgcgcac gcgtttggct gcgaccctgg tcatctttga
     1501 cgtgctgcac ctcgacgggg aggacctaac ccctctcccc taccaggacc ggcgcgccgc
     1561 gctcgaggac ctggctctcg gcggcagcaa cggtggttcc cgggtggtca cgtcgccggc
     1621 ctggacgaat atcgaggggt cggcggtgtt cgaggtcatt gagcagatgg gactcgaggg
     1681 ggtggtcgcg aaggcgacga tgtcgccgta ccaggccggg cgccgcagcc ggctgtgggt
     1741 gaagaccccc gtcaggcgca gcaacctgtt cgcggtggga ggctggattc catcatcgtc
     1801 gcgggcggat gcggtcggct cactgctagt cggtgggtac aacgtcgccg gcgaccttgt
     1861 ctactgcgga cacgtcagtt cggggttcag cgaccgtatg cgccgggccc tgtatgtgca
     1921 gctgtccgaa atccgttcgc ccgcttcacc gttccggtcg agccatcccg acccggatac
     1981 cgggcgggtg cgatgggtca agccggtcct ggtcggacgc gtggaatatc gggaattcac
     2041 cggccggctt cgccacgcag cttggaaggg cgcccaggcc gtcgacccgc ggctggccgt
     2101 cgtgcctcca ctgctgtagc cgcgccgcca agagtggtga ttcagcgcca gtagtcggga
     2161 agcgtctgca tgtgctgctg gtagtccggg ctgtctaggc gcgcggtaac gatgtctttc
     2221 atggtgccgg ccggccatcg gttgatgtgg tcgcggtacc aggtaagcca gtcgacgtcg
     2281 tcgccgccgg cgcgcaatcc ggcgcgatag gacgccccga gttcgtcgcg ggccacgacg
     2341 tagaggtctg gcttgttcgc tgcgctgagt ccgaggcgac atcggtggag tcgatcgccg
     2401 tctctagtcg cagcatttgt cgaccgcgtt gacgccctgg gatcgcagac gaagtgcgct
     2461 ggcgagggtc ttgtcgcggt cctgggtgct ggtgacttgc aattgcaggg ccagggcgcg
     2521 gtcctcgtgc aaggcctgac gggcggcgga gtaccaggcg cttagcgcgt cccgccagct
     2581 cgatgcgtgg ccgggttccc aagcggcgta gtccatctgg cgaaggtctt cgatcgccag
     2641 caggtcgccg tcggtgtaga tgtggtgggc agttgcggat cgctgcgggg tgtcgagtgt
     2701 gtcgcgccag tcaggtttga gcgcgccgtt tcgggcaagg gccaggatct ggcgcaacgc
     2761 ttgggtccgc aacacgcggt agtgccgttc ggcggctcgg ctgaaatgac cgccatcgcg
     2821 cgccgcgtag tcaaatgcca tgcaacccgg cctctcccct actcgacatt gtcgctcgac
     2881 gttggtcgga caccagcgaa cggtcatctg tggtctctgt gcgtgcggct gggcgcccgt
     2941 cgaagttgac gaaggtgcat gccggcgtcc tcggcggccg atacaccgtg gttccgcaag
     3001 ctcggcaggt aaacgtgcga tgagggccct ggccccgctc ggcgcatggc gcgcattgct
     3061 gggtgcctac aagtgcccgg tagcccccgc gcagcgggtg gccgcgggca ggttggcggt
     3121 gggggcggtt cttcgtagcc ccgggcagtg cgccgaagcc gagccacacg ttcgatcata
     3181 cgttcggcca ctcccctccc ccgccctgag aattgtcggc gcttgggcga cctcaccgct
     3241 cacccgctca ttgtttcacc gctgcggatg tgcgcggagc gccaagattc aacctgccgg
     3301 gccgcatcag cgtccgcatc gctgtagagc ctggcctggc agccaaccac tgccctcccg
     3361 gcgctgctat ttcggtcatg ccgcgcacca cgccccaaat ccggtgtagg ctgcggtaat
     3421 tcagcgtgcg ggcagttgat gcagtgcgtc agtcctgcac ctttcctcca ctgaaattag
     3481 tgcacggagc taccatccgc cccggttagg cttgggtttt gacggaagat gcgcggtgag
     3541 cacccggaca tagacagaga tgacatgaac cataaccagc acggtgcggg cgcgtcgcgt
     3601 aatgagatga acggcgcgag aagggaatta gcgtccgcac cgtcacctgg cggtcgtggc
     3661 ggggtgacgt ccgctgccga agagcgccgt gccgtgcggt ttttctgggt ggtgctgatg
     3721 ctggcgtcct cggcgtcggt ggctggcaac gtgacccacg cagtgtggaa tgcgtctggc
     3781 ccggcaatgg ttgtcgcagc gctggcggcc ctcgtggctc cgctgtccct gctcggggcg
     3841 acccactcgg tcggggtgct cgccagggtg cgcaccaaca gcttcacgtt ctgggcgagc
     3901 atggccatca cgatcgcggt cgttgtgtgc gcattctggc tgtcgtttca cgcgctgcgc
     3961 tcggtggcgg tgaatctcgc ccaggtcgac ccagggattg cctggatgat gccggtgtgc
     4021 attgatttgt ctatcgccgg gtccaccctg gcgcttctgt cgctgtcgcg accgagcgcg
     4081 gctgcgccga ccgcatcgca gactgcgcga gccccgcgtg caagcaccaa tggttctacg
     4141 tcgccggcca gcgctgccac gaacggtcgc gcgccggcag ccacgtcggg agtgcaggac
     4201 gtcgttacgc ggccaaggcc cgttggcggg ccccataaca acggccatgg gagggctggt
     4261 gttgcgctag aggaacgccc gacgctcgtc gcggtcggtc aaatggtgga ggcggtgacg
     4321 gcagccgaga tgcacccgga agcggaggcg cgttggagcg gtgtcgccga aaccctggtg
     4381 cgcgagaagc gcaccaagat tccggccagg gtggtggcta aggtgctggc gctgcgagag
     4441 gcccagacgc cgccgagcac cattgggcgc ctgttgaatg tgcaccattc ggcggtgcgg
     4501 cgcattctgg acggcgccga cgacgtcgac cggtcaggaa gcggcccggg cgatgtacca
     4561 ggtatggcgc aatagttcgg gaacgctcat atccgagccg gtcggcctcg cgttgcagtt
     4621 cgaccctgtc ggtcctggtg aagcgcactg ggatggactc agtaagccgc cggttctcgg
     4681 accggggttt gcgcccgggg ttgggcctgg ccacattcag ctcctcagtc gcggcagtct
     4741 tcgtgcgcgc cccgccggcg cgcatgcaca cactg
//
LOCUS       DASWBF010000396         4623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_770144,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000396 DASWBF010000000
VERSION     DASWBF010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770144"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..336)
                     /locus_tag="VF874_14760"
     CDS             complement(7..336)
                     /locus_tag="VF874_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX9177279.1"
                     /translation="MSGLGYTLAAVLAVIAVCALERRVLRTRLFRRPAYWISMAIVLG
                     FQIPVDGWLTKLSAPIVLYNEQHTTGVRFPFDIPIEDFLFGFALVTAVLLLWERRRPQ
                     IEMADQR"
     gene            complement(333..674)
                     /locus_tag="VF874_14765"
     CDS             complement(333..674)
                     /locus_tag="VF874_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX9177280.1"
                     /translation="MDSWQYLIVLGACLLITAPLELFGRGVYRQPRRLLGAVLPVAVV
                     FLIWDAIAVAADVWWYNPRYILGLIAPGDLPLEEILFFVVIPLCGLLTYSAVDDILAI
                     VSRRRPEARAR"
     gene            complement(677..1621)
                     /locus_tag="VF874_14770"
     CDS             complement(677..1621)
                     /locus_tag="VF874_14770"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX9177281.1"
                     /translation="MIGSELDAASVRDPSLREAFRQCREINARDGKTFFLATRLLAPR
                     QRPAVHALYGFARRADDILDDFDARGTELRSEQLQALSTQLFNSLAENQPCDDDPTLT
                     AVVHTARKYDIGWDLFDDFLASMRMDLTVTDYPDRVALERYMYGSAEVIGLQLLPILG
                     TVGPREDAAPYAAALGKAFQLTNFIRDVDEDLSRGRVYLPADELAAHGVDRELLSWCQ
                     INRRTDPRVRRALMDQHAAARQVYAEARPGIEMLAPASRPCVAVAWTLYSEILKRIEE
                     DDFQIFGQRATVGKARRLTVAGGGLVRSWTARLRHGSR"
     gene            complement(1618..3153)
                     /gene="crtI"
                     /locus_tag="VF874_14775"
     CDS             complement(1618..3153)
                     /gene="crtI"
                     /locus_tag="VF874_14775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX9177282.1"
                     /translation="MRIVEGRTDRVVIVGAGLSGLSAALYLAGRGRNVTVVERGAVPG
                     GRVGRADVSGYRLDTGPTVLTMPDLIDDALSAVGETLTDRLELLPVHPAYRAMFADGN
                     TLEVHTEADAMFAEIERFSGRREADAYLRLRSWLTELYRTEFDGFIAANYDSPLSLVT
                     PQLARLVALGAFRRWEKVMRRFLHDERLLRVFTFQALYAGVPPQSALAVYAVIAYMDT
                     IAGVYFPRGGMRAVPDAMAAAAADAGVEFVYESTVSELEWSGSRVTAVHTQAGHRIAA
                     DAVVLTNELPDTYRLLGRTPRRLLKLRPAPSAVVAHVGCRAVGDASGQHPTADTVGHH
                     TIVFGEAWRQTFGDIIEQGRVMRDPSLLVTRPTAGDPSLAPPGRDLLYVLAPAPNTEV
                     GRVDWDATSSAYVDEMLGAVTARLPQLGEALEILKVVTPADWGRQGMAAGTPFALAHT
                     LGQTGPFRPSNTVRGVDNVLLAGSSTVPGVGVPTAILSGRLAADRITGVSTRQPDREV
                     YQR"
     gene            complement(3153..4241)
                     /locus_tag="VF874_14780"
     CDS             complement(3153..4241)
                     /locus_tag="VF874_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9177283.1"
                     /translation="MHGYRGHTGNGIPVHWRDEVRTAVRAHLSEFVARQCRTDLGAAK
                     VDVAGDILSGFVDGGKYVRSTFMYLGWLCGAGEDDSALRASASLELFHTFALMQDDVM
                     DESALRRGRPAAHVMFSRWHRDRALTGASDRFGESAAVLLGDLCLVWAAKMLRESGIG
                     ADALARAWPRYDDMRIELAIGQFADLVNDSRAFPTLEDVLDVLRRKSGNYTVRRPLEI
                     GGAMSGCAPEVIDALGGYGAAIGEAFQLRDDLLGVFGSPTLTGKSVGTDLAAQKATSV
                     VVAAHQLADTALRRQLTELMSTSELSPADAERWRTLITVSGAVRWIEQLINERLTRAL
                     GCLDSVEIPEMTRAALEDMAVICTERAA"
BASE COUNT          828 a         1574 c         1478 g          743 t
ORIGIN      
        1 tctgactcat cgctgatctg ccatctcgat ctgcggccgg cgccgctccc agagcaacaa
       61 cactgcggtc accaacgcga agccaaacag gaaatcctcg atcggtatgt cgaacggaaa
      121 ccgaaccccg gtggtgtgct gttcgttgta gaggacaatg ggcgcactga gtttggtcaa
      181 ccagccgtcg accgggatct ggaagcccag cacgatggcc atggagatcc agtacgcggg
      241 ccggcggaac agcctggtgc gcagcacccg cctttccagc gcacacaccg cgatcaccgc
      301 aagcaccgcg gcaagcgtgt agcccagacc actcatcgcg ccctggcctc cgggcgccga
      361 cggctgacga tggcgaggat gtcatcgacc gccgagtagg tcagcaaccc gcacagcggt
      421 atcaccacga agaacaggat ttcctccaac ggcagatcac cgggtgcgat caggcccagg
      481 atatagcgcg ggttgtacca ccagacgtcg gcggcaaccg cgatggcgtc ccagatgagg
      541 aacaccacgg ccacgggaag cacagcgccg agcaaccgcc tcggctgccg atacacacca
      601 cggccgaaca gctcgagcgg agctgtgatc agaaggcatg cgcccaggac gatcaagtac
      661 tgccagctgt ccacactcac ctactcccgt gtcgcagtcg cgcagtccac gaccgtacga
      721 gaccgccccc ggcgacggtg agtcgccggg ctttccctac ggtggcccgc tggccgaaga
      781 tctggaagtc gtcttcctcg atgcgcttca agatctccga gtacagcgtc cacgccactg
      841 cgacgcatgg acgcgacgcc ggggccagca tctcgatacc cgggcgggcc tcggcgtaca
      901 cctgacgcgc cgcggcgtgc tgatccatca gggctcgccg cacccggggg tcggtgcgcc
      961 gattgatctg acaccaactc aacaactccc ggtctacacc gtgcgcagcg agttcgtcgg
     1021 cgggcagata aaccctgccg cggctcaggt cctcgtcaac gtccctgatg aagttggtga
     1081 gctgaaatgc ctttccgagt gcagccgcgt acggtgccgc gtcttcgcgc gggccgacag
     1141 tgccgaggat tggcagcagc tgcagtccga tgacttctgc agacccgtac atgtagcgct
     1201 ccaacgccac ccgatccggg tagtcggtca ctgtcaggtc catccgcatc gatgccagga
     1261 agtcgtcgaa gagatcccag ccgatgtcgt acttgcgtgc ggtgtgcacc accgccgtca
     1321 aggtggggtc gtcatcgcat ggctgattct ccgccaggga attgaacagc tgcgtggaca
     1381 gtgcctgaag ctgctcggag cgcaactcgg tgccacgggc atcgaagtcg tcgaggatgt
     1441 cgtcggcgcg ccgagcgaac ccgtaaagcg catggaccgc agggcgctgc cggggcgcca
     1501 gcaaccgggt ggccaggaag aaggtcttgc cgtcgcgggc gttgatctct cggcactgcc
     1561 ggaatgcctc gcgcagggac ggatcccgca cgctggcggc gtcgagctcg gatccgatca
     1621 tcgctggtac acctcgcgat ccggctggcg tgtcgatact ccggtgatcc ggtcggccgc
     1681 cagccgcccg gacaagatcg cggtgggtac gcccactccc ggcaccgtcg aggaaccggc
     1741 caacagcacg ttgtcgacgc cgcggacggt gttggacggc cggaacggtc cggtctggcc
     1801 gagggtgtgc gccagcgcga acggcgttcc cgcggccatg ccctgccggc cccagtcggc
     1861 cggtgtcacc accttcagta tctcgagggc ttcaccgagc tggggcagtc gggcggtgac
     1921 cgcaccgagc atctcgtcga cgtaggccga tgaggtggcg tcccaatcta ctcgtcccac
     1981 ttcggtattc ggcgcgggag ccaggacgta gagcagatca cgaccgggtg gcgccagact
     2041 tgggtccccg gcggtcggtc gggtcaccag cagtgacgga tccctcatga cccggccctg
     2101 ctcgatgatg tcaccgaacg tctgtcgcca cgcttcgccg aacacgatcg tgtggtggcc
     2161 gactgtatcg gcggtggggt gctgccctga ggcgtcgccg accgcacggc agccgacatg
     2221 ggccaccacc gccgacggcg ccggacgcag tttcaacaac cggcgcggtg tgcggcccaa
     2281 cagtcggtag gtgtctggaa gctcgttcgt gagcacgacg gcgtcggcag cgatgcgatg
     2341 tcctgcctgc gtgtggaccg ccgtgacccg cgatccgctc cactcgagtt cggaaaccgt
     2401 tgattcgtaa acgaattcga ccccggcgtc agctgcggcg gcggccatcg cgtcgggcac
     2461 tgcccgcatc ccgccgcgcg ggaagtacac cccggcaatg gtgtccatgt acgcgatcac
     2521 cgcgtacacc gccagcgcgc tctgcggcgg aacccccgcg tacagcgcct ggaaggtgaa
     2581 cacccgaagc agccgttcgt cgtggaggaa tcggcgcatc accttctccc agcgcctgaa
     2641 tgctcccagc gcgaccagcc gggccagctg cggagtcacc agggacagcg gcgaatcgta
     2701 gttggcggcg atgaacccgt cgaactcggt gcggtacagc tcggtcagcc acgaacgcag
     2761 gcgcaggtag gcatcggctt cgcggcgccc ggagaacctt tcgatctcgg cgaacatcgc
     2821 gtccgcctcg gtgtgcacct ccagcgtgtt gccgtcggcg aacatcgcac ggtaggccgg
     2881 gtgcaccggc agcagttcaa ggcgatcggt cagtgtttct ccgaccgcgg acaaggcatc
     2941 gtcgatgagg tcgggcatcg tcaacaccgt cggaccggtg tcgaggcggt agccggagac
     3001 gtcggcccgc ccgactcgtc cgccgggcac cgcgccgcgc tcgacgacgg tgacgttgcg
     3061 tccgcgcccg gccagataca gcgctgcgga caatcccgac agccccgcgc cgacgatgac
     3121 gactcgatcg gttcttccct cgactatgcg catcaggcag ccctttcggt gcaaataacg
     3181 gccatgtctt cgagtgctgc gcgggtcatt tcgggaatct cgacagagtc gagacaaccc
     3241 agcgcgcggg tcaggcgctc gttaatgagc tgttcgatcc accgcacggc gccgctgacg
     3301 gtgatcagcg ttcgccagcg ttctgcgtcg gcggggctca gctccgaggt gctcatcagc
     3361 tcggtgagct gtcgccgcag cgcggtgtcg gccagctgat gggcggcgac gacgacgctg
     3421 gtcgccttct gggctgccag gtcggtgccc accgatttac cggtcagggt tggtgacccg
     3481 aatacaccga gcaggtcatc gcgcaattgg aaggcctcgc cgatggcggc accgtagccg
     3541 ccgagcgcat cgatgacctc tggcgcgcac ccggacatcg cccctccgat ctccaatggt
     3601 cggcgcaccg tgtagttgcc cgactttcgc ctcagcacgt cgagcacgtc ctcgagcgtc
     3661 gggaaggcac gcgaatcgtt gaccaggtcg gcgaattgac cgatcgccag ctcgatgcgc
     3721 atgtcgtcgt atcggggcca cgctcgcgcc agcgcgtcgg caccgatgcc actctcacgc
     3781 aacatcttgg ccgcccacac caggcagaga tcgccgagca ggacagccgc cgactcaccg
     3841 aatcggtccg aggcacccgt gagcgcgcgg tcccgatgcc agcggctgaa catcacgtgc
     3901 gcagcgggcc gcccccgacg cagcgccgat tcatccatca cgtcgtcctg catgagagca
     3961 aacgtatgga agagctcgag gctggccgat gcgcgcagcg ccgaatcatc ttcccccgca
     4021 ccacacaacc agcccaggta catgaacgtc gaccgcacgt acttcccgcc gtcgacaaac
     4081 cccgagagta tgtcgccggc gacgtcgacc ttggccgcgc ccaggtcggt tcggcactgt
     4141 cgggccacga actcgctgag gtgtgcccgc acagcggtgc ggacctcgtc gcgccagtga
     4201 accggaatac cgttgccggt atggccacgg tatccgtgga tgcgccggtg tgatgaaatg
     4261 agcgcccgcg ttccgccgtc acctccgctc aacgtcgcac ccccgcgaac tggacggagc
     4321 agacatcccg cagaagtcga tctcgcatgt acggcaactc agcgttcccc attcacactt
     4381 cggtccggcc gtttggcggc aacaccgagc ccgctccatt gcggactggg cgtgcctagg
     4441 gcattcgtag ccgagacggc tttggttggg ttgacggtca agaaaatttc ctcaagccct
     4501 tgacctggac tttcggtgac caggagcgct tttgctgaca ccgatgtgtc ggtgcagccg
     4561 cgccagaggc gcgggcgccc accagttcca ccgtccgaga attcgcatga aggccggcac
     4621 cag
//
LOCUS       DASWBF010000397         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_45820, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000397 DASWBF010000000
VERSION     DASWBF010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45820"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(628..1668)
                     /locus_tag="VF874_14785"
     CDS             complement(628..1668)
                     /locus_tag="VF874_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9177284.1"
                     /translation="MTMVSGFDVHRKQITFDYVDDDGVVRWGQIRPATRTSLRGWLGE
                     HCPEGHAEFALEGCTGWRYVSEELAAAGVGVHLGDPAEIAALRGPKKRAKTDRADARL
                     LRTLLLEGRFPGSWIPPAHVLEVRTLGRLYCTLMDERRAWQQRIHAQLFHQGCPPITA
                     LLSAAGRDALACAELSAAGRQYVDTALRRIDELTAEIDPLRTQLVNFARHQRGCQALQ
                     ARQYGIGWLCAAIIWAEIGDARRFARSDQVVRFAGMDVTVYSSDGKRSPGHLSRQGSP
                     ELRWATFEAAKCAARRGSPDYAYYHKLAARPHSGKNPTLAVERKILRRCYHILRELGD
                     AALAFPPQEVAA"
     gene            complement(1759..2526)
                     /locus_tag="VF874_14790"
     CDS             complement(1759..2526)
                     /locus_tag="VF874_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HEX9177285.1"
                     /translation="MFTQTVTKRVLGSGLIDVLTGPHGVDRYTELVTPTWSLSDARAK
                     VVSVEGRTPRSVTLTLAPNDAFRGFKAGQHVNLTVEIDGRRHTRCYSPASAEGSPLIE
                     LTVGLHDGGLVSTYLHRDARPGMVVGLDGVGGEFVLPQTPPRRILFVSGGSGITPVMS
                     MLRTLTAEGYDGEIAFVFSDSSIERTDDGWPLLDQAEAAGLTPASGCRMGICHTCTRR
                     KTRGVVKNLITGAVSAVDEEDVQICVSVPVGDVAVAL"
     gene            2597..3271
                     /locus_tag="VF874_14795"
     CDS             2597..3271
                     /locus_tag="VF874_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9177286.1"
                     /translation="MNSRTPSSRSRRSGSSSPRMKVSREERKEATRRAIVAAALKLLD
                     DRSFSALSLREVTREAGIVPAAFYRHFESMDALGLVLIDESFRVLRDMLRSARAGKLD
                     PNRVIESSVEIVVGSIAQQREHWRFIGRERNSGVSVLRYAIRTEIRLITAELATDLAR
                     FPGLNDWSTEDLNILASLFVNSMIVIAEAIEDAQDASAQEEIKRMAVKQLRMIVVGVA
                     GWRSNT"
BASE COUNT          607 a         1127 c         1182 g          582 t
ORIGIN      
        1 agtagacata cccgagaacc agagcgtacg acaacgggtc ggcgctgcgg gccatggcca
       61 cgccgtggcg caggtcgtct gaccatcccg gacgacccag gcaataccgg cccagagccc
      121 gcgtcgtaaa ggcgagcgct agcggagacc cgataatgaa gtttccttat ctgtgttggg
      181 gacccccgta aaggtgggga tctgagttgt tttcgttgtt tggcctcgtt cggccatgtg
      241 ccttgtggcg gtggaccgct gtcggggtcg aggtggagcg cccgcagcgc agcgaggacg
      301 aacgacctcg atgccgacaa ggatccgccg catactgtgg ccggccagga cgaggtaaac
      361 acgttgtggt tcaagtcatc tgcggcctcc cgttgaacgg atgggtgtcc acgttgcgcg
      421 tgccggcgcc cagccttatc tgggtccacg gacgagggtc cggcgacatg atgattgatg
      481 gggtgcccga cgggcgggct gccgaagggt gcggccgctc attctttccg ggaagtccac
      541 cccacagcgg ggtggcgaca gggacactgc gggagctggc cgcgggcatc agctgttgtg
      601 tgggcagggc acgtgcgcaa cgcatggtta ggcggccacc tcctgcggcg ggaacgccag
      661 ggcggcgtcg ccgagctcac gcagaatgtg ataacagcgg cgcaggattt tgcgctccac
      721 cgccagggtc gggttcttgc cactgtgcgg gcgggcagcc agcttgtggt agtaggcgta
      781 atcgggggaa ccacgccgag cggcgcactt ggccgcctcg aaggtggccc agcgcagctc
      841 cggggagccc tgccgggaca gatgccccgg tgagcgtttg ccgtctgagg agtacacggt
      901 gacgtccatg ccggcaaagc gcaccacctg atcggagcgg gcgaatcggc gcgcatcgcc
      961 gatctcggcc cagatgatcg ccgcgcataa ccacccgatg ccgtactggc gcgcctgcag
     1021 cgcctgacag ccacgttggt gtcgggcgaa gttcaccaat tgtgttcgca gcgggtcgat
     1081 ctcggcggtc agctcgtcaa tgcgccgcag cgcggtgtcg acgtactggc gtcccgccgc
     1141 cgacagctcg gcacaagcga gcgcatcgcg gccggccgcc gacaacagcg cggtgatcgg
     1201 tgggcagccc tggtggaaca actgggcatg gatgcgctgc tgccaggcgc ggcgctcgtc
     1261 catcagggtg cagtacaacc ggcccagggt gcggacctcc agcacatgtg cgggcggaat
     1321 ccacgacccc gggaaccgac cctccaacag cagcgtacgc agcagccgcg catcggcgcg
     1381 gtcggtcttg gcccgcttct tgggaccccg caacgctgcg atctcggccg gatcccccaa
     1441 atgcacgccc acgccggcgg ccgccagctc ctcgctcaca taccgccatc cggtgcagcc
     1501 ctccagcgcg aactccgcgt gcccctccgg gcagtgctca cccagccaac cacgcagcga
     1561 cgtgcgggtt gccgggcgga tctgacccca ccgcaccacg ccgtcgtcat ccacatagtc
     1621 gaacgtgatc tgcttccgat gcacgtcaaa cccgcttacc atcgtcattg ggacctcctc
     1681 aagctcccga aaaaaacact gacgcaaact caacgagcgt ctgagctgag ggggtcccca
     1741 tcacatgtca tctttcgttc acagcgcgac tgcgacatcg ccgaccggga cggacacgca
     1801 gatctgcacg tcctcctcgt cgactgccga caccgcgccg gttatcaggt tcttgacgac
     1861 gccgcgggtc ttgcggcgcg tgcaggtgtg gcagatgccc atccggcatc cgctcgcggg
     1921 cgtcaggccc gcggcctcag cttggtcgag cagcggccag ccgtcgtcgg tgcgctcaat
     1981 gctgctgtcg ctgaagacga atgcgatctc gccgtcgtag ccctcggccg tcagcgtgcg
     2041 cagcatcgac atcacgggcg tgatgccgct gccgccggac acgaacagga tccgccgggg
     2101 cggagtctgc ggcagcacga actcgccacc gacgccgtcg agtccgacca ccattccggg
     2161 ccgggcatcg cggtgcaggt atgtcgacac caggccgccg tcgtgcaggc cgacggtcag
     2221 ctcgatcagc ggcgacccct cggcgctggc aggtgaatag cagcgggtgt ggcgccggcc
     2281 gtcgatctcg accgtgaggt tgacgtgctg gccggccttg aagccacgaa aagcgtcatt
     2341 cggcgcgagc gtcagcgtga cactgcgcgg cgtgcggccc tcgaccgaca cgaccttcgc
     2401 gcgggcgtcg gacagcgacc atgtcggtgt caccagctcg gtgtagcggt cgacgccatg
     2461 gggtccggtg agcacgtcga ttaagccgga gcccagcacg cgcttggtaa ccgtttgagt
     2521 gaacatatgt acacagtgtg tcgcttacag gcagaaagtc aatgagaaag accaggttgt
     2581 ggtactgttc acaacagtga acagtcgtac gcctagctca cggtcgcgca ggtccggctc
     2641 gtcgtcgccc cggatgaagg tgtcgcgtga ggagcgcaaa gaggccaccc ggcgcgcgat
     2701 cgtggcggcc gcattgaagc ttctcgacga ccgcagtttc agtgccctca gccttcgcga
     2761 agtgacccgc gaggcgggca tcgtgccggc cgcgttctac cggcatttcg agtcgatgga
     2821 cgcgctcggt ctcgtcctga tcgacgagtc gttccgcgtg ctgcgcgaca tgttgcggtc
     2881 ggcgcgcgcg ggcaagctcg acccgaaccg ggtgatcgag tcgtcggtcg agatcgtggt
     2941 cggcagcatt gcgcagcagc gagagcactg gcgcttcatc ggccgcgagc gaaacagcgg
     3001 ggtgtcggtg ttgcggtacg cgatccgcac cgaaatccgg ttgatcacag cggaactcgc
     3061 gacagacctg gcgcgcttcc cagggctgaa cgactggagc accgaggatc tcaatattct
     3121 cgccagcctg ttcgtcaact cgatgatcgt gatcgccgag gccatcgagg acgcgcagga
     3181 cgcctcggcg caggaggaga tcaagcgcat ggcggtcaag cagctgcgga tgatcgtcgt
     3241 cggggtggcc ggctggcgca gcaacaccta gcgccgaaag cgaacccagg cctgtgattc
     3301 ggcgcaacca acgacctgtg gggtcttttc ctgatcgtca attgtggtga aaccggtggc
     3361 cgaccgggcg taagatcctg gtctgacctg ggtcggggaa gatccgaggg aggcctgcga
     3421 tggtgggcat gattcaccgt ttggcaatcc tcggcgcggc cgcgttggct ccgctcgcat
     3481 tcgtgacact ggttgcgc
//
LOCUS       DASWBF010000398        12521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1292020,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000398 DASWBF010000000
VERSION     DASWBF010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12521
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292020"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2137)
                     /locus_tag="VF874_14800"
                     /pseudo
     CDS             complement(1..2137)
                     /locus_tag="VF874_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
     gene            2270..2584
                     /locus_tag="VF874_14805"
     CDS             2270..2584
                     /locus_tag="VF874_14805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX9177287.1"
                     /translation="MPVVVVATMTAKPESVDTVRDACKRAIEAVHQEPGCELYALHEA
                     NGTFVFVEQWADEDALKTHSTARAIATLFGAVGEHLDGAPDIEMLQPVPAGEAGKGRL
                     RA"
     gene            2586..3404
                     /locus_tag="VF874_14810"
     CDS             2586..3404
                     /locus_tag="VF874_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEX9177288.1"
                     /translation="MASLEGKVAFITGAARGQGRAHAVKLASEGADIIAVDLCDQIAS
                     VPYPMATADDLAATVKLVEDTGARIVAREGDVRDGGSLAAALQAGLDEFGGLDIVVAN
                     AGIAPMQSGEDGWRDVIDVNLTGVFHTVEVARPAMVEQGRGGSIVLISSVAGLVAMGS
                     PDPGAMGYTAAKHGIVGLMREYANLLARHSIRVNSVHPTGVNTPMINNEFIQQWLEQA
                     NSQQDAPRDMGNALPVPAVEAEDIANAVAWLVSDAARYVTGVTLPVDAGAVVKR"
     gene            3527..4909
                     /locus_tag="VF874_14815"
     CDS             3527..4909
                     /locus_tag="VF874_14815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9177289.1"
                     /translation="MNRLSSVDAAFWFAETPSWHMHGSGLAICDPSEAPDFSFDAVRD
                     LIASRLPEIPQLRYRVSGAPLGLDRPWFVEDDELDLDFHIRHIRVPRPGGRAQLDELV
                     ERLMSHRLDRSRPLWQLWFIDGLEDGRVAILTKMHHAVIDGMSGAGLSEILFDVTAQS
                     RPPAGDVDRSLVGVGIPRFEQRALGSIVNVTVMTPYRLLRIARQTLAQQLATWRLPNK
                     PPRYFEAPTTRFNAPLTPHRRVSGCQVELDRVKAVKNAFGVKVNDVVLALVSGALRHY
                     LLVRGELPHRPLVAQVPVSTRGDSMEVGNQISSMTVGLATDVADPAKRMRAIYANSQG
                     AKEMTKALTAHQIMGLTETTPPGLLALAARAYTASHVGGNVAPINLVVSNVPGVDFPL
                     YLAGAVVEYMVPLGPLVMDVGLNITCFSYRGLLQFGFVTTPEIADDIDELAAEIEPAL
                     QKLEKAAAKL"
     gene            complement(4906..6582)
                     /locus_tag="VF874_14820"
     CDS             complement(4906..6582)
                     /locus_tag="VF874_14820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX9177290.1"
                     /translation="MMSDLTPPGPLAPGALRVTALGGISEIGRNMTVFEHLGRLLIVD
                     CGVLFPGHDEPAVDLILPDLRHIENRLDDVDALLLTHAHEDHIGAIPFLLKLRGDIPV
                     VGSKFTLALVAEKCREHRLKPTLIEVAAGQKHRHGVFDCRYFAVNHSIPDALAIAIDT
                     GAGTVLHTGDIKFDQLPLDRRPTDLPGMSRLGDTGVDLLLVDSTNAELPGVGPSESEI
                     GPALHRLIRSADGRVIVACFASNVARVQQIIDAAVSLGRRVSFVGRSMVRNMGIAREL
                     GFLKVAADDVVDIGAAELMSPEQVILITTGTQGEPMSALSRMSRGEHRSITLTPGDLI
                     VLSSSLIPGNEEAIYGVIDALSKIGARVVTNQQVRVHVSGHAHAGELLFLYNGVRPRN
                     VMPVHGTWRHLRANAALAASTGVPEESILLAENGVSVDLMAGRATISGAVPVGKMFVD
                     GLVLGDVGDATLGERLILSSGFVAVTVVVRRGTGRPAAPVHLHSRGFSEDPKALESVA
                     RKVEEELELLAAERVTEPTRIAQAVRRTVGKWVGEAYRRQPMIVPTVIEI"
     gene            complement(6579..7481)
                     /gene="dapA"
                     /locus_tag="VF874_14825"
     CDS             complement(6579..7481)
                     /gene="dapA"
                     /locus_tag="VF874_14825"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX9177291.1"
                     /translation="MSTSGMDVGAQLGTVLTAMVTPFDADGALDLPAAARLANHLIVS
                     GCDGLVVSGTTGESPTTTDDEKLALLRTVLEAVGDRARVIAGAGTYDTAHSVKLAKAC
                     EAEGAHGLLVVTPYYSRPPQAGLLAHFTAIADAVSLPVLLYDIPPRSVIPIDFDTIRR
                     LAYHPNIVGVKDAKGDLHGGAEIIASTGLAYYSGDDPLNLPWLAMGATGFISVISHLA
                     PGQVRDLLSAFESGDIATARKISVSLSPLCAAMRRLGGVTMSKAGLRLQGIDVGDPRL
                     PQVPATAEQTDELAADMRAAAVLV"
     gene            complement(7507..8259)
                     /gene="thyX"
                     /locus_tag="VF874_14830"
     CDS             complement(7507..8259)
                     /gene="thyX"
                     /locus_tag="VF874_14830"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX9177292.1"
                     /translation="MAETASLRVQLIAKTEFIAPPDVPWSTDADGGQALVEFAGRACY
                     QSWSKPNPRTATNASYLRHIIDVGHFSVLEHASVSFYITGVSRSCTHELIRHRHFSYS
                     QLSQRFVPEHDSQVVLPPGIDDDPELQELFLAAADASRETYAQLLAKLEAKFADEPNA
                     VLRRKQARQAARAVLPNATETRIVVTGNYRAWRHFIAMRASEHADVEIRRLAIACLRQ
                     LADLAPAVFADFEITTLADGSEVATSPLATEA"
     gene            8343..8585
                     /locus_tag="VF874_14835"
     CDS             8343..8585
                     /locus_tag="VF874_14835"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9177293.1"
                     /translation="MKTTVNLPDELIREAQELARQQRTTLRELIETGLRAVVRQRSNE
                     SAFMLEDASIDGDGLQPAFRGASWEQIRNTIYEHPA"
     gene            8582..9019
                     /locus_tag="VF874_14840"
     CDS             8582..9019
                     /locus_tag="VF874_14840"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HEX9177294.1"
                     /translation="MIAVDTNILVYAHRRDAEFHATAAERVKALAEGRSAWAIPWPCL
                     YEFFAITTHPRIYGPPSTPNQAIAQIEAWLASPPLTLLSETPGYWDRLKAMLATGKVA
                     GPIVHDARIAALCAAHGVRELWSADRDFGRFGSSLTVRNPLVG"
     gene            complement(9088..10293)
                     /locus_tag="VF874_14845"
     CDS             complement(9088..10293)
                     /locus_tag="VF874_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX9177295.1"
                     /translation="MSRLTADQARRVGVAAQGFTEPAPRGPVTRAHLRRLITRIQVLQ
                     LDSVSVAVRAHYAPVFSRLGPYDREVLDRAAWSHSARSPRLLVEYWAHEAALMSVDDW
                     PLLRWRMRQYRHGRWGTHIVKANPQLADDIADAVAELGPSTAGEIEAHLNAEPRRGKG
                     PWWNRSDTKWVAEALFASGVLTTATRVGFARHYDLTERVLPPEVLARHIPDDEAVREL
                     TLRAATALGVGTEADIRDYFRLPAGHAKPAVAALVASGELERVDVDGWSAPAYLRAGQ
                     AVPRTDRGTALLCPFDPLIFFRPRVQRLFGFHYRIEIYTPARQRQYGYYVWPFLLDGR
                     LVGRVDLKRVDDALHVVGAFVEPGESPVRVAATLAAEMQSMASWRDLSRVTVGESGDL
                     AAALRAAIG"
     gene            complement(10290..10772)
                     /locus_tag="VF874_14850"
     CDS             complement(10290..10772)
                     /locus_tag="VF874_14850"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX9177296.1"
                     /translation="MSVSLIWAQSATGVIGRADSIPWRLPEDLSRFKDLTMGHTVVMG
                     RRTWESLPARVRPLPGRRNVVLSRQAGYAAEGADVVTSLDDALTDDEVWVIGGAQIYA
                     LALPLAIRCEITEVDVDMPRQDDDAVAPVLDEGWVAQDGQWQISDAGLRYRFSSYRRR
                     "
     gene            complement(10769..11569)
                     /locus_tag="VF874_14855"
     CDS             complement(10769..11569)
                     /locus_tag="VF874_14855"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX9177297.1"
                     /translation="MPIATPYEDLLRLVMERGTAKSDRTGTGTRSLFGQQLRYDLSAG
                     FPLITTKKVHLKSVIYELLWFLRGDSNVGWLHDHGVTIWDEWASDTGDLGPIYGVQWR
                     SWPTPSGEHVDQISAALELLRTDPDSRRIIVSAWNVSDIPHMALPPCHAFFQFYVADG
                     RLSCQLYQRSADLFLGVPFNIASYALLTHMMAAQAGLGVGEFVWTGGDCHIYDNHVEQ
                     VTAQLSRDPRPYPELVLAQRDSLFDYTYDDVAILNYDPHPAIKAPVAV"
     gene            11612..12352
                     /locus_tag="VF874_14860"
     CDS             11612..12352
                     /locus_tag="VF874_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9177298.1"
                     /translation="MPSVSATVTTPDGECPVTLHTPNGTGPWSAVVMFPDAGGVRDTF
                     HDMADTLAGFGYAVLLPDVYYRHAGWRPFDMKTVFNDEQERRRLFGMIGTMTPDMMSA
                     DAAALFDFLAQRPEVRGERFGVCGYCMGGRMSVIVAGRQPDRVAAAGSIHGGGLVTDG
                     ADSPHLLADRMTATVYVGAAANDASFTPEQAEILDKALEAAGVPHTIETYHAGHGFAV
                     PDNAPYDADAAEQHLLALRRLFDSTLPG"
BASE COUNT         2091 a         4176 c         4204 g         2050 t
ORIGIN      
        1 gttccttgca tgcggcgacg acggcatgga tctcctcgtc ggtgatgtag gcgccctgca
       61 ggcggatcgg cttgttggcg cccatcggga tgaagagccc gtcgcccatg ccgatcaact
      121 tctcggcgcc tggctgatcc agaatgacgc gactgtcgat cagcgatgac gtggcgaatg
      181 ccagtcgcga cggcacgttc gtcttgatca ggccggtgac cacgtccacc gacggacgct
      241 gggtggccag caccaggtgg atgccggccg cgcgggcctt ctgggtgatc cgcacgaccg
      301 cgtcctcgac gtcgcggggc gcggtcatca tcaggtcggc gagctcgtcg acgatcgcca
      361 cgacgtacgg gtacggctgg tactcgcgct ggctgcccag cggtgtggtg atggcgccgg
      421 atctcacctt ctggttgaag tcgtcgatgt ggcgcacacg actggcctgc atgtcctggt
      481 agcgctgctc catctcctcg acgagccacg ccagcgccgc cgccgccttc ttcggctgcg
      541 tgacgatcgg cgtgatcaga tgcggaatgc cttcgtacgg cgtgagttcc accatcttcg
      601 ggtcgatcag gatcatcctg acctcctccg gggtggcgcg cgccagcagc gagatcagca
      661 tcgaattgat gaagctcgat ttgcccgagc cggtggagcc ggcgaccagc agatgcggca
      721 tcttggccag gttccacgag atgtattcgc cctcgatgtc cttgccgagt ccgatcacca
      781 acgggtgatg gtcgcgccgg gtcgaggggt cggtcagcac gtcggccaac cgcaccatct
      841 cgcggtcgat gttgggcacc tcaatgccga cggccgactt gccggggatc ggcgccagca
      901 tccggacgct ttcggttgcc accgcgtagg cgatgttctt ctgcaattgg gtgatcttct
      961 cgaccttcac gcccggcccg agctcgacct cgtagcgcgt gaccgtcggg ccgcgagtgc
     1021 agccggtgac cgcggcgtcg accttgaact gcgccagcac cgagttgatc gcgtcgatca
     1081 tgtggtcgtt gcccgcgcta cgccgcttcg gtggatcacc ggcgatcagc agatcaagcg
     1141 acggcaaggt ataggggccg tcgacgactc ggtccaggtc ggcgaagttc gacttcctcg
     1201 agcgccggcg ggtcggacga gcctcgactg gcggcccctc ctccaccacg gggggctcgg
     1261 ggatggtcgg tgtgtcgtcc tcgagcggat attcgtcgta gccgggctcc catgcctggg
     1321 cctcgtcgtc gtagtcctcc tgcggggccc ctgccaggcc gcggaccgcg aacatcgccc
     1381 gcagcgtcga cggcagctcg cgaaccgtgg tgccggacag cagcagaacc ccgaacagca
     1441 cgccgatgaa caacagcggc gcggcgatcc acgcggtcac accgtctgac agcgggccgc
     1501 cgatcgcgaa gccgatgaaa cccgcggcgt gttgccggcc tgcgggtgtc agcggggagc
     1561 cggcccacag atgccacagc cccagcgccg gcagcgcgac catcgccgag ccgagcacca
     1621 gccgcggccg cgactcgggg tcgggctgcg tccgcgtcaa cgtcacggcg acgatcaatg
     1681 cgacgatcgg cagcagcacg accgccgagc cgatcagagt ccgcagcgtc gtgtcgaccc
     1741 actggcccac cggccgcgca gcgtcgaacc agctgctggc cgcgatcagc accgcgacac
     1801 caagcagcgc cagcgcgatc ccgtcgcggc gatggccggg ctcgatgtcg cgggcgcgtc
     1861 ccaccgagcg ggcggcggtg ccggtggcct tggccaccat cagccatgtc gcctgcgtgc
     1921 cgcggccgac cgcgagcgcg cccgccgtga ccggaccgtg accggaggat ttgcgccgtt
     1981 tgaccggttt cttgcggggc gccgcccgcg ggccgcttcg cgaactggcc tttgacctgg
     2041 tcgttcggct cccggaacgg gcagcggtct tgctaggcat gaacgcaagc ctaatcgcat
     2101 gcgcctcatt cgcactatcc gccacactgg tcactgattt gacgggccat gtgcgccgaa
     2161 agctacgttt tgcagagctc tactcgcact tccgcttcaa aacatcgctc tcgggacggc
     2221 tgagtaaggt gatcccgtca catcgccctc accgaggagt tcttcgccca tgcccgtcgt
     2281 cgtcgtcgcc accatgaccg ccaagcccga gtccgtggac accgtgcggg atgcctgcaa
     2341 gcgggcgatc gaagcggtgc atcaggaacc ggggtgcgag ctgtacgcgc tgcacgaggc
     2401 caacgggacg ttcgtgttcg tcgaacagtg ggccgacgag gacgcgctga agacgcatag
     2461 caccgcgcgt gcgatcgcga cgctgttcgg ggcggtcggt gagcacctcg acggcgcgcc
     2521 ggacatcgag atgctgcagc ccgttcccgc gggggaggcc ggcaaggggc ggctgcgcgc
     2581 ctgacatggc atcgctcgaa ggcaaggttg cgttcatcac gggcgccgca cgcggccagg
     2641 gccgcgccca tgcggtgaag ctggcctccg agggcgccga catcatcgcg gtcgacctgt
     2701 gcgaccagat cgcgtcggtg ccgtacccga tggccacggc agacgacctg gccgcgaccg
     2761 tgaagctggt ggaggacacc ggcgcgcgca tcgtggctcg tgagggtgac gtgcgagacg
     2821 gcggatcctt ggcggcggcg ctgcaggccg gcctcgacga attcggcggg ctcgacatcg
     2881 tcgtcgccaa tgccggcatc gccccgatgc agtcgggcga ggacggttgg cgcgacgtga
     2941 tcgacgtcaa cctcacgggc gtgttccaca cagtggaggt cgcgcgaccg gcgatggtgg
     3001 agcagggccg cggcgggtcg atcgtcctga tcagttccgt ggcaggcctg gtcgccatgg
     3061 gcagccccga cccgggagcg atgggttaca ccgcggcaaa gcacgggatc gtgggactaa
     3121 tgcgcgagta tgcgaatctg cttgctaggc atagcattcg ggtcaattcg gtgcacccga
     3181 ccggggtgaa cacgccgatg atcaacaacg agttcatcca gcagtggcta gagcaggcca
     3241 actcgcagca ggacgcgccg agggacatgg gtaacgccct gccagtgccg gcggtggagg
     3301 ctgaggacat cgccaacgcg gtggcatggc tggtgtccga cgcggcacgg tacgtcacag
     3361 gggtgacgct gccggtggac gcgggggcag tcgtcaagcg ctgagcctcc ggcgttgact
     3421 ctgcggccag ggcgccgtcc actcgcactt tcgcgccctg gatgcagagt cgatcgcgct
     3481 ggccgttttc cggtcggcat cgctgcgcga accataggct gatcccgtga accggctgtc
     3541 cagtgtcgat gcagcgttct ggttcgccga gacgcccagc tggcatatgc atggcagcgg
     3601 gctggcaatc tgcgatccgt ctgaagcgcc ggacttttcg ttcgatgccg tccgcgacct
     3661 gatcgcctcg cgcctgcccg agatcccgca actgcgatac cgggtgagcg gcgcgccgct
     3721 ggggctggac cggccgtggt tcgtcgagga cgacgagctc gacctcgact tccacatccg
     3781 gcacatccgc gtgccgcgac cgggtggtcg cgctcagctc gacgaactcg tcgagcgtct
     3841 gatgtcacac aggctcgacc gcagtagacc gctgtggcaa ttgtggttca tcgacggtct
     3901 cgaggacggg cgcgtcgcca tcctgaccaa gatgcatcac gctgtcatcg atggcatgtc
     3961 gggtgcgggg ttaagcgaaa tcctgttcga cgtcactgcg cagtccagac cacctgcggg
     4021 cgacgtggac aggtcgttgg taggcgtcgg tatccccagg ttcgaacaac gggcacttgg
     4081 ctcgattgtc aatgtcaccg tgatgacgcc ctaccgcctt ctacggatcg cccggcagac
     4141 gctcgcacag caactggcca cgtggcgact acccaacaag ccgccgcggt acttcgaggc
     4201 cccgacgaca cggttcaacg ctccgctcac gccgcaccgg cgcgtcagtg gttgccaggt
     4261 tgaactggac agggtgaagg cagtcaaaaa cgcatttggt gtcaaggtga atgacgttgt
     4321 gctggcactg gtttcgggcg ccttgcgtca ctatctgctg gtacgaggtg agctccccca
     4381 ccggccgctg gtcgcacagg tgccggtatc gacccggggc gacagcatgg aagtgggcaa
     4441 ccagatcagc tccatgacgg tggggctggc caccgacgtc gccgacccgg ccaagagaat
     4501 gcgggccatc tacgccaatt cgcagggcgc gaaagaaatg accaaagcgt tgacggcaca
     4561 tcagatcatg ggtctaacag agacgacgcc acccggcctg ttggcgttgg ccgcccgcgc
     4621 gtacaccgca agccacgtcg ggggtaacgt cgccccgatc aatctggtgg tctccaacgt
     4681 tcccggagtc gactttccgc tctatctcgc gggagcagtc gtcgaataca tggttccgct
     4741 aggaccgttg gtgatggatg tgggtctcaa catcacctgt ttcagctacc gcggattgct
     4801 gcaattcggc ttcgtcacga cgcccgagat cgccgacgac atcgacgaat tggccgctga
     4861 aatcgaaccc gcactgcaga agttggaaaa ggccgcggcc aagctctaga tttcgatgac
     4921 ggtcggcacg atcatcggct ggcgccggta ggcctccccc acccacttgc cgacagtgcg
     4981 ccgcaccgct tgcgcgatcc gcgtgggttc cgtaacacgt tctgcggcaa gcagttccag
     5041 ttcttcttcg accttgcgcg cgacggattc cagtgccttg gggtcttcgg agaaccctcg
     5101 ggagtgcaga tgaaccgggg ccgcgggccg gcctgttccg cgacgcacca ccaccgtcac
     5161 cgcgacaaac ccggacgaca agatgagccg ctcaccgagc gtcgcgtccc cgacgtcacc
     5221 cagcaccaga ccgtcgacga acatcttgcc caccggcacc gctccggaaa tagtggcgcg
     5281 gccggccatc agatcgacac tgacaccgtt ctccgccaac aggattgact cttcgggtac
     5341 accggtactt gccgcgagcg cggcgttggc gcgcagatgc cgccatgtgc cgtgcaccgg
     5401 catcacgttg cggggacgca caccgttgta caggaacaac agctccccgg cgtgggcgtg
     5461 ccccgaaaca tgcacgcgca cttgttgatt agtgaccacc cgggcgccga tcttcgacag
     5521 ggcgtcgatg acgccgtaga tcgcttcctc gttgccggga atcagcgacg acgacaacac
     5581 gatcaggtcg ccgggagtca gcgtgatgct gcggtgctcg ccgcgcgaca tccgcgacag
     5641 cgccgacatc ggctcgccct gcgtcccggt ggtgatcagt atcacctgct cgggcgacat
     5701 gagttcggcg gcgccaatgt ccactacgtc gtcggcggcc accttcagga agccgagctc
     5761 gcgggcgatg cccatattgc gaaccatcga gcggccgacg aaggacaccc tgcggcccag
     5821 cgacacggcg gcgtcgatga tctgttgcac gcgagcgaca ttcgaggcga aacaggcgac
     5881 gatgacgcgt ccgtcggcgg aacggatcag tcggtgcagc gcaggtccga tctcgctttc
     5941 cgacggtccg acgccgggca actcggcgtt ggtggagtcc accaggagca ggtcgacgcc
     6001 ggtgtcgccg aggcgcgaca tgccgggcag gtcggtcgga cggcggtcca gcggcagttg
     6061 gtcgaacttg atgtcaccgg tgtgcagcac cgtgcccgcg ccggtgtcga tcgcgatcgc
     6121 aagcgcgtcg gggatcgagt ggttgaccgc gaaatagcgg cagtcgaaca ccccgtgccg
     6181 atgcttctgg cccgcggcga cctcgatcag agtcggcttg agccggtgct cacggcactt
     6241 ctcagccacc agcgccagcg tgaacttcga gccgacgacg gggatgtcgc cgcgcagctt
     6301 cagcaggaac ggaatcgcgc cgatgtggtc ctcgtgcgcg tgggtgagca gcagcgcgtc
     6361 cacgtcatcg aggcggttct cgatgtggcg caggtcgggc aggatcaggt cgacggccgg
     6421 ctcgtcgtgc ccggggaaca gcacgccgca gtcgacgatc agcagtcggc cgagatgctc
     6481 gaaaacggtc atgttgcgac cgatttcgct gataccgccc agagcggtca cccgcagagc
     6541 gccgggcgcc aacggccccg gcggcgtgag gtcggacatc aaaccagcac cgcggcggcg
     6601 cgcatgtcgg cggccaactc gtcggtctgt tcggcggtgg ccggcacttg gggaagccgt
     6661 ggatcaccga cgtcgatacc ctgcagccgc agaccggcct tcgacatcgt cacgccgccg
     6721 agccgcctca tcgcggcgca cagcgggctc agtgacacgc tgatcttccg cgcggtcgcg
     6781 atgtcgccgg attcaaaggc ggacaacaga tctcgcacct gaccgggagc caggtgggag
     6841 atcacgctga tgaatcccgt cgcacccatc gccagccacg gcaggttcag cgggtcgtca
     6901 ccggagtagt acgccagccc ggtcgatgcg atgatctccg cgccaccgtg caagtcacct
     6961 ttggcgtcct tcacgccgac gatgttcggg tggtaggcga gcctgcggat ggtgtcgaag
     7021 tcgatcggga tcaccgatcg cggcgggatg tcgtagagca acacgggcag cgacaccgcg
     7081 tcggcgatcg cggtgaagtg cgcgagcagc ccggcctgcg gcggccgtga gtaatacggc
     7141 gtgacgacca gaagaccgtg ggcgccttcg gcttcgcacg ccttggcgag cttgacactg
     7201 tgggcggtgt cgtaggtgcc ggccccggcg atcacgcggg cgcggtcgcc gaccgcttcg
     7261 agcaccgtgc gcagcagagc caacttctcg tcgtcggtag tcgtcggaga ctcgccggtg
     7321 gtgcccgata ccaccagccc gtcgcagcca gagacaatca ggtggttggc cagccgcgcg
     7381 gcggcgggca ggtccagagc gccgtccgcg tcgaagggcg tcaccatcgc ggtcagcacg
     7441 gtgcccagtt gcgcgccgac gtccattccg ctggtgctca cggggttcaa ggttacccgg
     7501 ctgccctcac gcttccgtcg ctagcggaga cgtggcgacc tcgctgccgt cggccagcgt
     7561 ggtgatctcg aagtccgcga acaccgcagg ggcgaggtcg gcgagctggc gcaggcaggc
     7621 gatcgcgagg cggcggatct ccacgtcggc gtgttcgctg gctcgcatcg cgatgaaatg
     7681 ccgccacgcg cggtagttgc cggtgacgac gatgcgggtc tcggttgcgt tcggcagcac
     7741 ggcccgggcg gcttgacgcg cctgcttgcg ccgcagcacg gcgttgggtt catccgcgaa
     7801 cttggcctca agtttggcca gcagctgtgc gtaggtctcc cggctggcgt cggccgcggc
     7861 caggaagagc tcctgcagtt cggggtcgtc gtcgatgccc ggcggcagca ccacctgcga
     7921 gtcgtgttcc ggcacgaacc gctgcgacag ctgcgagtag gagaagtgcc ggtgccggat
     7981 cagctcgtgg gtgcacgagc gggagacgcc ggtgatgtag aacgacaccg acgcgtgctc
     8041 gagcaccgag aaatgcccga cgtcgatgat gtgtcgcagg taggacgcgt tcgtcgccgt
     8101 ccgcgggttc ggcttcgacc agctctggta gcaggcgcgt ccggcgaact cgaccagcgc
     8161 ctggccgccg tccgcgtcgg tgctccacgg cacgtccggc ggcgcgatga actcggtctt
     8221 cgcgatgagc tgcactcgca gcgacgcggt ctcggccacg gtgtagaccc tatccggcgg
     8281 ccgttcgccg gcctgtacag tcgctagtat ggctgtacgg cttctacgat atggttgacc
     8341 gtatgaagac gacggtgaat ctccctgacg agctcatccg agaggcgcag gagctcgccc
     8401 gtcagcagcg caccactttg cgggagttga tcgaaactgg tttgcgtgcc gtggtcaggc
     8461 aacgctcgaa cgagtccgct ttcatgcttg aagacgccag catcgacggt gatggtctac
     8521 aaccggcgtt ccgcggtgcc tcgtgggagc agatccggaa caccatctac gagcaccccg
     8581 catgatcgcc gtcgacacca acatcctggt gtacgcgcat cgccgcgacg ccgagtttca
     8641 cgccacggcc gccgaacggg tcaaagcgct tgcggaaggc agatcggcat gggcgatccc
     8701 ctggccttgc ttgtacgaat tcttcgcgat caccacacac ccgaggatct atggcccgcc
     8761 cagcacgccg aatcaagcga ttgcgcagat cgaagcctgg ctggcgtcac caccgctgac
     8821 gttgctcagc gaaacgccgg gttactggga ccggctgaag gcaatgctcg ccaccgggaa
     8881 agtcgccggt ccgattgtgc atgacgcgcg gattgctgcg ctgtgcgcag cgcacggtgt
     8941 gcgcgagtta tggtctgccg accgcgattt cggccggttc ggttcgtcgt tgaccgtgcg
     9001 aaacccattg gtcggctgat cccgcgactg tgtggtcttg tacggccacc gcggcgtgtc
     9061 gcgtacaaca ccgcacagtc gcggcgttca cccgatcgcg gcgcgcaacg cggccgcgag
     9121 atcgccgctc tcgcctacgg tcacccggct cagatcccgc catgacgcca tcgactgcat
     9181 ttcggccgcg agcgtggccg cgacgcgcac cggtgactcc cctggctcga cgaaagcgcc
     9241 cacgacgtgc agtgcgtcgt cgacgcgctt caagtcgacc cggcccacca gccggccgtc
     9301 gagcagaaac ggccacacgt agtagccgta ttgacgctgc cttgcgggtg tgtagatctc
     9361 gatgcggtag tgaaacccga acagccgctg cacccgcgga cggaagaaga tcagcgggtc
     9421 gaacgggcac aacaaagccg tgccgcggtc ggtgcgcggc acggcttggc cggcccgaag
     9481 ataagccggc gcagaccacc cgtcgacgtc gacgcgttcc agctcacccg acgcaaccag
     9541 cgcagcgacc gcgggcttgg cgtgcccagc cgggagccgg aagtagtcgc gaatgtcggc
     9601 ctcggtgccc acccccaacg ccgtcgccgc ccgcaacgtc agctcgcgga cggcctcgtc
     9661 gtcggggatg tgccgcgcca gcacctcggg cggcagcacc cgctcggtca ggtcgtagtg
     9721 ccgcgcaaag cccacccgcg tcgccgtggt gagcactcct gacgcgaaca gcgcctcggc
     9781 cacccacttg gtgtcgctgc ggttccacca cgggcccttc ccgcggcgcg gctccgcatt
     9841 caggtgcgcc tcgatctcgc cggctgtcga cggcccgagt tcggcgaccg cgtcggcaat
     9901 gtcgtcggcg agttgcgggt tggccttgac gatgtgcgtg ccccagcggc cgtgccggta
     9961 ctggcgcatc cgccaccgca gcagcggcca gtcgtcgacc gacatcagcg cggcttcgtg
    10021 tgcccagtac tcgaccagca gccgcggcga gcgcgcgctg tggctccacg ccgcccgatc
    10081 cagaacctcg cggtcatagg ggccgagtcg gctgaacacc ggcgcgtagt gcgcgcgaac
    10141 tgccaccgac accgaatcaa gctgcagcac ttggattctc gtgatcagcc gacgcagatg
    10201 cgctctggtg accggtcccc gtggcgccgg ctcggtgaag ccctgagcgg caacgcccac
    10261 ccggcgggcc tgatcggctg tcagtcggct catcgccgcc ggtagctgct gaaccggtag
    10321 cgcagaccgg cgtcgctgat ctgccactgc ccgtcctgag ccacccagcc ctcgtcgagc
    10381 accggcgcga ccgcgtcgtc gtcctggcgc ggcatgtcga cgtcgacttc ggtgatttca
    10441 cagcggatcg cgagcggcag cgccagcgca tagatctgtg cgccgccgat cacccacacc
    10501 tcgtcgtcgg tcaacgcatc gtcaagcgac gtcacgacgt cggcgccctc tgccgcgtag
    10561 ccagcttgcc gggacagcac gacgttccgc cggcccggca gcggccggac ccgcgccggt
    10621 agcgactccc acgtgcgccg gcccatcacc acggtgtggc ccatcgtcag gtccttgaac
    10681 cgggacaggt cctcgggtag ccgccatgga atgctgtcgg cgcggccgat cacccccgtg
    10741 gccgactgag cccagatcag gctcacgctc atacagccac gggcgccttg atcgccgggt
    10801 gcgggtcgta gttcaggatc gcgacgtcgt cgtaggtgta gtcgaacagc gaatcacgct
    10861 gcgccagaac aagttcgggg tatggccgtg ggtcgcgact gagctgcgcg gtgacctgct
    10921 cgacgtggtt gtcgtagatg tggcagtcgc cgccggtcca gacgaactcg ccgacgccaa
    10981 ggcccgcctg cgcggccatc atgtgggtca gcagcgcgta gctggcgatg ttgaacggca
    11041 cgccgagaaa caggtcggcg ctgcgctggt acagctgaca gctcagccgt ccgtccgcga
    11101 cgtagaactg gaagaacgca tggcacggcg gcagcgccat atgcgggatg tcggagacgt
    11161 tccatgccga cacgatgatc cggcgcgagt ccgggtcggt gcgcagcaac tccagcgccg
    11221 cgctgatctg gtcgacgtgc tcgccggacg gcgttggcca ggaccgccat tgcacgccgt
    11281 agatcggccc gagatcgcct gtgtccgagg cccattcgtc ccagatcgtg acaccgtggt
    11341 cgtgcagcca gccgacgttg gagtcgccgc gcagaaacca cagcagctcg tagatgaccg
    11401 atttcaggtg caccttcttg gtggtgatca gcgggaagcc ggcggacagg tcgtagcgca
    11461 gctgctggcc gaacaggctt cgagtgccgg tgcccgtgcg atcggatttc gccgttcccc
    11521 gctccatcac cagtcgcagc agatcctcgt agggcgtggc gatgggcacg ccggtcagct
    11581 tacttgggaa gcccacgagt aaaacggaac catgccgagc gtctccgcga ctgtcaccac
    11641 acctgacgga gagtgccccg tcaccctgca cacacccaac ggcaccggcc cgtggtcggc
    11701 cgtcgtcatg ttccccgacg ccggcggcgt ccgcgacacg ttccacgaca tggccgacac
    11761 attggcgggc ttcgggtatg cggtgctgct gcccgacgtc tactaccggc acgctggttg
    11821 gcggccgttc gacatgaaaa ccgtcttcaa cgatgagcag gagcgcaggc ggctgttcgg
    11881 catgatcggc actatgacgc cggacatgat gtccgccgac gcggccgcat tgttcgactt
    11941 ccttgcgcag cggcccgagg tgcgcggcga gaggttcggc gtctgcggct attgcatggg
    12001 cggacgcatg tcggtcatcg tcgccggccg gcaacccgac cgcgtcgccg cggccggatc
    12061 gatccacggc ggcgggctgg tgacggacgg cgcggacagt ccccatctac tggccgaccg
    12121 catgacggcg acggtctacg tcggtgccgc ggcaaacgat gcgtccttca ctccggagca
    12181 ggcggagatc ctcgacaagg cactggaagc agcaggggtg ccgcacacga tcgagacgta
    12241 ccacgccgga cacgggttcg cggtgcccga caacgcgccg tacgacgccg acgccgccga
    12301 gcagcatttg ctggcgctgc ggcgattgtt cgattcgacg ctgcctggct agtgcttgcg
    12361 gcgccgcagc agcattgcca cgcttcgacg caggccgcgg aacgcataga acgcggtgac
    12421 cacgcagatg atgatgagcg cgatgagcat cggcagccaa ttgctggcgc gattctcttt
    12481 ggtccaccaa atgatcgtga acagcaccgc gcagacgaac a
//
LOCUS       DASWBF010000399         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_770769,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000399 DASWBF010000000
VERSION     DASWBF010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770769"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..309
                     /locus_tag="VF874_14865"
                     /pseudo
     CDS             1..309
                     /locus_tag="VF874_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402842.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
     gene            306..692
                     /locus_tag="VF874_14870"
     CDS             306..692
                     /locus_tag="VF874_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9177299.1"
                     /translation="MTSFARRRAQLNRKVTNPLVRPISGWVPAWSIVEHVGRRSGKTY
                     RTPVSVFRTDDGVAILLPYGTDTDWVRNVRAANGGQVKLSGKTFRVTSPRIVPTADAV
                     ETVKPPWGRIMKATGIESTLLLTRAS"
     gene            complement(676..1158)
                     /locus_tag="VF874_14875"
     CDS             complement(676..1158)
                     /locus_tag="VF874_14875"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9177300.1"
                     /translation="MTPLERIAPAFVDMAHSIVWATVATVDADGRPRTRILHPIWQWD
                     GSTLTGWIATVPTKLKHEHLAAHPEVSISYWTTNHDTCSAECLAQWYLDDDTCTTVWE
                     MFKNGPAPVGYDPAIIPQWHGGPTSDDFAALRLTPRRLRVMPATVLTKGEGEILSWHA
                     "
     gene            complement(1165..>1568)
                     /locus_tag="VF874_14880"
     CDS             complement(1165..>1568)
                     /locus_tag="VF874_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177301.1"
                     /translation="DAGPQSNYYVEAFTSAIMQLRRHRVLNRMITDEPAMTLQLAHRH
                     YPAAIKRMADALRVIFLTGFAERIGEQAVDELADTILRYALMALLLPSSRPLDTADDV
                     RVFATAHFLPSLPSALRERELATSSEEDRLT"
BASE COUNT          254 a          485 c          565 g          264 t
ORIGIN      
        1 gcccgcgttc gggattctcg agcatgtggg ccggcggtcc gggaagcgct atcgcacgcc
       61 gctgaacgtg ttcagcactg acgacggcgt ggcgatcctg ctcacttacg ggcccaaccg
      121 ggactggctg aagaatctga cgtccgcggg gcgcgggcga atcagcgcta cggcaagacg
      181 tttgaggtca ccgacccccg ggtgatgccg aaggcggtgg cagcgccgtc ggtcaggagg
      241 ctgtggcggc cgatcttctc tcgactgccg ttcgagtacg cactgctgtt gaaaaggact
      301 gacagatgac gagtttcgct cgacgacggg cgcaactgaa ccggaaggtc accaacccgc
      361 tggtgcggcc gatcagcggc tgggtgccgg cgtggtcgat cgtcgagcat gtcgggcgac
      421 gctccggcaa gacctaccga accccggtgt cggtcttccg gacggacgac ggcgtcgcga
      481 tcctgctgcc ctacggcacc gacaccgact gggtgaggaa cgtgcgagcc gccaacggcg
      541 gtcaggtgaa gttgagcggc aagacatttc gggtgacgag cccgcggatc gtgccgaccg
      601 ccgatgcggt ggagacggtc aagcccccgt gggggcggat catgaaggcg acgggcatcg
      661 agtcgacgct gctgctcacg cgtgccagct gaggatctcg ccctcgccct tcgtgagcac
      721 ggtggcgggc atgacccgca gcctgcgggg cgtcagccgg agcgcggcga agtcgtcgga
      781 cgtgggtccg ccatgccact ggggaatgat cgcggggtcg tagccgacgg gtgcgggacc
      841 gttcttgaac atctcccaca ccgtggtgca ggtgtcgtcg tcgaggtacc actgcgccag
      901 gcactcggcg ctgcaggtgt cgtggttggt ggtccagtag ctgatcgaga cctcggggtg
      961 cgcggcgagg tgctcgtgct tcagcttcgt cgggacggtg gcgatccagc ccgtcaacgt
     1021 ggacccgtcc cactgccaga tcgggtgcag gatccgggtg cgcggtctgc cgtcggcgtc
     1081 gaccgtggca accgtcgccc agacgatcga atgggccatg tcgacgaatg cgggcgcgat
     1141 gcgttccagc ggcgtcatgc gggcctacgt tagacgatct tcttcggatg acgtcgccag
     1201 ttcacgttcg cgcagcgccg agggcaagct cggcagaaag tgggcggtgg cgaacacccg
     1261 gacgtcgtct gcagtgtcca gcggccgcga tgacggcagc agcagcgcca tcaacgcgta
     1321 gcgcaggatc gtgtcggcga gttcgtcgac ggcctgctca ccgatccgct cggcgaaccc
     1381 cgtgaggaag atcacgcgca gcgcgtcggc catccgcttg atcgccgcgg ggtaatgccg
     1441 gtgcgcgagc tgcagcgtca ttgccggctc gtcggtgatc atccggttca gcacgcgatg
     1501 gcggcgcagt tgcatgatcg ccgacgtgaa cgcctcgacg tagtaattac tctgcggccc
     1561 agcgtctt
//
LOCUS       DASWBF010000400         8306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_164399,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000400 DASWBF010000000
VERSION     DASWBF010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164399"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2297
                     /locus_tag="VF874_14885"
     CDS             <1..2297
                     /locus_tag="VF874_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX9177302.1"
                     /translation="ETPAPAPAAPKSSSASRRVRARLARRMTAQRSAVNPVLEPLVAV
                     HKEIYPKADLTMLQRAYEVAEERHATQFRRSGDPYITHPLAVANILAELGMDTTTLIA
                     ALLHDTVEDTGYTLEALTADFGEEVGHLVDGVTKLDKVVLGSAAEGETIRKMVIAMAR
                     DPRVLVIKVADRLHNMRTMRFLPPEKQARKARETLEVISPLAHRLGMATVKWELEDLS
                     FAILHPKRYEEIVRLVADRAPSRDTYLTKVRTEIVSALNASKINAVVEGRPKHYWSIY
                     QKMIVKGRDFDDIHDLVGVRILCDEIRDCYAAVGVVHSLWQPMAGRFKDYIAQPRFGV
                     YQSLHTTVVGPEGKPLEIQIRTRDMHRTAEYGIAAHWRYKEAKGRNAVLHPHAAAELD
                     DMAWMRQLLDWQREAADPGEFLESLRYDLAAQEIFVFTPKGDVITLPAGSTPVDFAYA
                     VHTEVGHRCIGARVNGRLVALERKLENGEVVEIFTSKAPNAGPSRDWQQFVVSPRAKA
                     KIRQWFAKERREEALEAGKDAIAREVRRGGLPLQRLMNAESMGALARELRYADVSALY
                     TAVGEGHVSARHVVQRLLAQLGGDEAAEDELAERSTPATMPVRQRNTEDTGVAVPGAP
                     GVLTKLAKCCTPVPGDNIMGFVTRGGGVSVHRTDCTNAASLQQQSERIIDVEWAPSPS
                     SVFLVAIQVEALDRHRLLSDVTRVLADEKVNILSASVTTSGDRVAISRFTFEMGDPKH
                     LGHVLNVVRNVEGVYDVYRVTSAA"
     gene            complement(2340..2759)
                     /locus_tag="VF874_14890"
     CDS             complement(2340..2759)
                     /locus_tag="VF874_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9177303.1"
                     /translation="MRTPMVGQTAELSRTIGDNDIALFTLISGDRNPLHYDDTAARAS
                     RFGKIVVQGGVTTAILNAVVAEKLPGPGTVFLNVNWAFTAPVCPGDTITGRVEVIAVR
                     TDKPITELKTTVTRQDGTVVLDGTAVCYTMNMRGSEQ"
     gene            complement(2728..2922)
                     /locus_tag="VF874_14895"
     CDS             complement(2728..2922)
                     /locus_tag="VF874_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177304.1"
                     /translation="MTVQLVPVAADLVGWQYAFLLLAPGPVIGAGAMAALGAREPPHA
                     RKEKPNDLQLDAHSYGWPDR"
     gene            complement(2958..3986)
                     /locus_tag="VF874_14900"
     CDS             complement(2958..3986)
                     /locus_tag="VF874_14900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9177305.1"
                     /translation="MTALRPNRQRSLIALVQILALSVWFSAAAVVPNLRAEWGFGSMA
                     SGWLTTSVQIGFAAGAVASTLLHLADRITPQHLLAAGAACAATCTAALAVLAHGLCEA
                     IPLRFLTGVALAGVYPVGMKLMASWSESADRGQWFGILIGSLTLGSAFPHLISGLGPL
                     PWRTVMAIAATLSAAGAAIAVMAIVPGPHLDKRPIIPNPRHLMAMFAERGFRLTNAAY
                     FGHMWELYALWTWLPTFLRAGRQQTSDGGGTSTAIISFCAIGIAGTAGCLLGGWASDR
                     FGRSAAAVTALVISAACCVASPFFFTATTPALVMFLLVWGAAVIADSGVSPPRSARSR
                     TRASLAPH"
     gene            complement(3992..5173)
                     /locus_tag="VF874_14905"
     CDS             complement(3992..5173)
                     /locus_tag="VF874_14905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9177306.1"
                     /translation="MENHYDVIVVGGRVGGAATAMLLARAGLRVLVIEAARQGTDTLS
                     THALMRGGVTQLHRWGLLDAVIATGTPAIRATQFSYGDDTETVEIRTLDGISALRAPR
                     RTVLDALLLDAAREAGAEIRSPARVTRLLTDRGKVRGVEGVSRGTGTSFRATATITVG
                     ADGRGSMVAEAVDAEFQRRGTASGAIIYGYFPGLARDRYHWAYRPGVTAGVVPTGDGL
                     ACVWAGTPTARFDTMRSNSLDTSFRLLLAEAVPQVARSIADTPPAGPLRGFPGMPGYI
                     RSGGGPGWALVGDAGYYKDPITAHGITDALRDAELLAQAVLTAPRGGPAQLAALRDYQ
                     HIRNRLSEPLFDLTERIASYQWDLAELRGLLRALSRAMQPEVDALRGLDGPNERPRLA
                     G"
     gene            complement(5184..6416)
                     /locus_tag="VF874_14910"
     CDS             complement(5184..6416)
                     /locus_tag="VF874_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9177307.1"
                     /translation="MLKTTTVVVGAGHCGLAMSRCLAEKSIDHVVLERGEVANSWHTQ
                     RWDSLRLLTPNWMTRLPDYAYRGDDPDGYLSASETAQLITDYAKETAAPVQANTTVTS
                     VRSVERGYLVHTDQDTWHARTVVSASGGSAVARVPALQHAIPAGITTLTPTEYRNPSE
                     VPDGGVLVVGASASGIQIAEELHRSGRPVTLAVGEHVRMPRTYRGRDILWWMDATGLL
                     DERYDEIPDLPRASRLPSMQLVGTPERKTLDLNALRGIGVQLVGRLAGIRDGLAQFSG
                     SLANVFTLADLKLGRLLDTIDTWAAKAGLEDLGPPQRFAPTQVPSPARLTLDLRSGEI
                     QTIIWATGLRPDLSWLDVPIFDRKGRVDHDGGVTASPGLYLIGMPFLRRRKSTLIDGA
                     AADAADLTGHLVRYLDAT"
     gene            complement(6434..6994)
                     /locus_tag="VF874_14915"
     CDS             complement(6434..6994)
                     /locus_tag="VF874_14915"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9177308.1"
                     /translation="MTTLNTSVDNGVNVDALLGARNALADAPELAQFQWRTQVDWVNG
                     THSRSTVDTFYGLGEEQHHEAPFTYDVDHPLAFAGHDKGATPVEYVLVALGGCLTAGI
                     ASIAQRRGIQLRSVHATIEAGEDIQGILGVDPDIRNGFSGIRVTYKIDADATADEIRA
                     LVAQSQKRSAVYDIITNPTDVIVDVA"
     gene            7725..7967
                     /locus_tag="VF874_14920"
     CDS             7725..7967
                     /locus_tag="VF874_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177309.1"
                     /translation="MIKAERPAGPGTLHKGYAMSTISTMRTGHHRRADGSLVRVDVEL
                     GRWVGSLYTPDMRVKARIVGSDVDVHAWAIQSMTNG"
     gene            8111..8306
                     /locus_tag="VF874_14925"
                     /pseudo
     CDS             8111..8306
                     /locus_tag="VF874_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875500.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
BASE COUNT         1409 a         2607 c         2877 g         1413 t
ORIGIN      
        1 cggagactcc cgcgccggca cctgcggcgc cgaagtcctc cagcgcgtcg cggcgagtgc
       61 gcgcccgcct ggcgcgccgg atgaccgccc agcgcagtgc cgtcaatccc gtgctcgagc
      121 cgctggtcgc ggtgcacaag gagatctacc cgaaggccga cctgacgatg ctgcagcggg
      181 catacgaggt cgccgaggaa cgtcacgcca cccagttccg ccgctccggc gatccataca
      241 tcacccatcc gctggccgtc gcgaacattc tggccgaact cggcatggac accaccacgc
      301 tgatcgccgc gctgctgcac gacaccgtcg aggacaccgg ctacacactg gaggcgctga
      361 ccgccgattt cggcgaagag gtcgggcatc tggtcgacgg ggtcacgaag ctggacaagg
      421 tggtgctggg cagcgccgcc gaaggggaga ccatccgcaa gatggtgatc gcgatggccc
      481 gcgatccccg cgtgctggtg atcaaggtcg ccgaccgact gcacaacatg cgtactatgc
      541 ggttcctgcc accggagaag caggcccgca aggcccgcga aacactggaa gtcatttcgc
      601 cactggcaca tcggctcggc atggcgaccg tcaagtggga gctcgaggac ctgtcgttcg
      661 cgatcctgca cccgaagcgc tacgaggaga tcgtgcggtt ggtggccgac cgggccccct
      721 cgcgtgacac ctacctgacc aaggtgcgca ccgagatcgt gtcggcgctg aacgcgtcga
      781 agatcaacgc ggtcgtcgag ggcaggccca agcactactg gtcgatctac cagaagatga
      841 tcgtcaaggg ccgcgacttc gacgacatcc acgacctggt gggtgtgcgc atcctgtgtg
      901 acgagattcg cgactgctac gccgctgtgg gcgtggtgca ttcgctgtgg cagccgatgg
      961 ccgggcggtt caaggactac atcgcccagc cgcggttcgg ggtgtaccag tcgctgcaca
     1021 ccacggtggt gggccccgag ggcaagccgc tggagataca gatccgcacc cgcgacatgc
     1081 accgcaccgc cgagtacggc atcgcggcgc actggcgtta caaggaagcc aaggggcgca
     1141 acgcagttct tcatcctcac gctgccgccg agctcgacga catggcatgg atgcgtcaac
     1201 tgctcgactg gcagcgggag gccgccgacc cgggcgaatt cctcgagtcg ttgcgttacg
     1261 accttgcggc gcaagagatt ttcgtgttca cccccaaggg ggacgtgatc acgctgccgg
     1321 ccgggtccac accggtcgac ttcgcctatg ccgtgcacac cgaagtcggc caccgatgta
     1381 tcggcgccag agtcaacggc cggctcgtcg cgctggagcg caagctcgaa aacggcgaag
     1441 tggtagagat tttcacgtcc aaggcgccca acgccggccc gtcgcgagac tggcagcagt
     1501 tcgtggtgtc gccacgcgcg aaggcgaaga tccggcagtg gttcgccaag gagcgccgcg
     1561 aggaagcgtt ggaggcgggt aaggacgcga ttgcgcgtga agtgcgccgc ggcggacttc
     1621 cgttgcagcg gttgatgaat gccgagtcga tgggtgcttt ggcgcgcgag ctgcgctacg
     1681 cggacgtgtc cgcgttgtac acggcggtgg gcgagggcca cgtgtcggcg cggcacgtgg
     1741 tgcagcggct gctggcgcag ttgggcggcg acgaggccgc cgaggacgag ctggccgaac
     1801 ggtcgacgcc cgccacgatg ccggtgcgtc agcgcaacac cgaagacacc ggcgtggcgg
     1861 tgcccggcgc gcccggcgtg ctgaccaagc tggccaagtg ctgcacgccg gtgccggggg
     1921 acaacatcat gggcttcgtc acccgcggcg gcggagtcag cgtgcacagg acggactgca
     1981 ccaacgccgc gtcacttcag cagcagtccg agcggatcat cgacgtcgag tgggcgccgt
     2041 cgccgtcgtc ggtgttcttg gtggccatcc aggtggaggc actcgaccgg caccggctgc
     2101 tgtccgacgt cacccgggtg ctggccgacg agaaggtcaa catcctgtcc gcgtcggtga
     2161 ccacatccgg tgaccgcgtg gcgatcagcc gcttcacgtt cgagatgggc gaccccaagc
     2221 atctgggcca cgtgctcaac gtcgtgcgca atgtcgaggg cgtctacgac gtctaccggg
     2281 tgacctccgc agcctgactt cgccgcaagc atcacgacgc accgcatttt gcggcagcat
     2341 cattgctcgc tgcctctcat gttcatcgtg tagcacaccg ccgtaccgtc gagcactacc
     2401 gtgccgtctt gccgggtgac cgtggtcttg agctcggtga tcggcttgtc ggtacggacg
     2461 gcgatgacct ctacccggcc ggtgatcgtg tcgccggggc aaaccggagc cgtgaaagcc
     2521 cagttgacat tgaggaacac cgttccaggg ccgggcaact tctcagccac aaccgcattc
     2581 aggatcgcag tggtgacacc gccctggacg acaatcttgc cgaagcggga cgcccgggcc
     2641 gcggtgtcgt cgtagtgcag ggggttgcgg tcgccgctga tgagtgtgaa gagtgcaatg
     2701 tcattgtcac cgatggtgcg cgacagttca gcggtctggc caaccatagg agtgcgcatc
     2761 aagctgaagg tcattgggtt tctccttacg tgcgtgaggg ggttcgcgtg ccccgagcgc
     2821 cgccatcgcc cccgcgccga tcaccggacc cggtgccagc agcaggaagg cgtattgcca
     2881 accgacgagg tcggcggcga cgggtacgag ctggaccgtc actacggtca acaggaagcc
     2941 gaccgccgtt tgtgcggtca gtgcggtgcc aacgaggctc ttgttcgcga tctcgctgag
     3001 cgaggtggag aaacaccgga gtcggcgatg acggccgcgc cccacacgag caggaacatg
     3061 accagcgcgg gtgtcgtcgc ggtgaaaaag aacggggagg cgacgcagca ggctgcgctg
     3121 atgacgagcg cggtcaccgc ggcggcgcta cgtccaaagc ggtccgacgc ccatccgccg
     3181 agcaggcatc cggccgtgcc ggcgattccg atcgcgcaga acgaaatgat ggcggtcgag
     3241 gtgccgcccc cgtcgcttgt ttgctgccgg ccagctcgaa ggaaggtagg cagccaggtc
     3301 catagcgcgt acagctccca catgtgcccg aagtaggccg cgttggtgag ccggaagccg
     3361 cgttcggcga acatcgccat gagatgccgg ggatttggga tgatcggcct cttgtccaga
     3421 tgcgggccgg gcacgatggc cattaccgcg atggcggccc cggctgcgga caacgtcgcg
     3481 gcaatggcca tcaccgttcg ccagggcagc ggcccgagac cgctgatgag atgagggaac
     3541 gcggagccga gagtcaacga gccgatcagg attccgaacc attggcctcg atcggcggat
     3601 tcagaccagg aggccatcag cttcatgccc accgggtaga cgccagcgag tgccaccccg
     3661 gtgaggaatc tcagtgggat ggcctcgcag agaccgtgcg cgaggacggc gagcgccgca
     3721 gtgcacgtgg cggcgcatgc ggcgcccgcg gcgagcaggt gctgcggtgt gatgcggtct
     3781 gcgagatgga gcagtgtcga ggcaactgcg ccggcggcga agccgatctg cactgacgtg
     3841 gtcagccatc ccgaggccat agagccaaag ccccactctg cgcggaggtt cggtacgacc
     3901 gcggcggccg agaaccagac cgacaatgcc agaatctgga ctagtgcgat gagggatcgt
     3961 tgcctgtttg gacgtaacgc ggtcatgtcg gctacccggc gagacgcggt cgttcgttag
     4021 ggccgtcgag cccgcgcagt gcgtcgacct cgggctgcat ggcgcggctc agcgcccgaa
     4081 ggagcccgcg cagttcggcc agatcccatt ggtagctggc gatgcgctcg gtgaggtcga
     4141 aaaggggctc ggacagccgg tttcggatgt gttggtagtc acgcagcgcg gcgagttgcg
     4201 ccggtccacc gcgtggtgcc gtgagcactg cctgggcgag caattcggcg tcgcgcaggg
     4261 cgtcggtgat gccgtgtgcg gtgatggggt ccttgtagta gccagcgtca ccgaccagcg
     4321 cccagcccgg accgccgccg gagcggatgt agccgggcat gccggggaag ccgcggagcg
     4381 gaccggcggg cggcgtgtcg gcgatggatc gagccacctg cggtactgct tctgcgagca
     4441 gcaaccggaa ggacgtgtcg agactgttgg accgcatggt gtcgaaccga gcggttgggg
     4501 tgcccgccca gacgcaggcc aatccgtcac cggtcggcac cacgcccgcg gtgactcccg
     4561 gtcggtaggc ccagtggtag cggtcgcgcg cgagcccggg gaagtagccg taaatgatcg
     4621 cgcccgatgc cgtgccgcgg cgctgaaact cggcgtcgac cgcctcggcg accatcgagc
     4681 cgcgcccgtc ggcgccgacg gtgatcgtgg cggtggctcg gaacgacgtg ccggttccgc
     4741 ggctgacgcc ctcgacacca cgtactttgc cgcggtcggt cagcaggcgg gtgacgcgag
     4801 cgggactgcg gatttcggcg ccggcttcgc gcgccgcgtc gagcagcagc gcgtcgagca
     4861 ccgttcgccg tggggcccgc agcgcggata ttccgtcgag ggtgcggatt tcgaccgtct
     4921 cggtgtcatc gccgtagctg aactgggtcg cgcgaatggc gggggtgccg gtggcgatga
     4981 ccgcgtcgag cagcccccag cggtgcagtt gcgtcacgcc gccgcgcatg agcgcatgcg
     5041 tagacaaagt gtcggtgccc tgtcgggcgg cttcgatgac cagcacccgc agtccggcgc
     5101 gcgcaaggag catcgcggtg gcggcgcccc cgacgcgccc gccgacgacg atcacgtcgt
     5161 agtggttttc catggggtgc tccttacgtg gcatcgaggt agcggacgag gtggccggtg
     5221 agatcggcgg catcggcggc ggcgccgtcg atgagtgtcg acttccggcg ccgcagaaac
     5281 ggcatgccga tcagatagag gccgggactg gcggtcacgc cgccgtcgtg gtcgactcgg
     5341 cccttgcggt cgaatatcgg gacatccagc cacgacagat ccggccgcaa tccagtggcc
     5401 cagatgatcg tctggatttc gccggagcga aggtcgagtg ttagtcgcgc gggggaggga
     5461 acctgggtgg gggcaaaccg ttgcggcgga ccaaggtcct ccagaccggc tttggcggcc
     5521 catgtgtcga tggtgtcgag cagccgtccc agcttcaggt cagccagcgt aaagacgttg
     5581 gccagcgagc cggagaattg tgcaagccca tcccggatac cggccagtcg ccccacgagt
     5641 tgcacaccga tgccgcgcaa ggcgttgagg tccagggttt ttcgctcggg tgtgccgacc
     5701 aactgcatcg acggcagtct gctagctcgg gggaggtcgg ggatctcgtc gtagcgctcg
     5761 tcgagcagcc ctgttgcatc catccaccag aggatgtccc tgccgcgata ggtgcgcggc
     5821 attcgtacgt gctcgccgac ggccagcgtg accggacgcc cggagcggtg cagttcctct
     5881 gcgatctgga tcccgctcgc cgaagcgccg acgacgagga cgcccccatc gggcacctca
     5941 ctggggttgc ggtactccgt cggcgtgagg gtggtgatgc ccgcgggaat ggcgtgctgc
     6001 agcgccggca cgcgtgccac ggccgagccg ccggaggcgg agaccaccgt gcgtgcgtgc
     6061 catgtgtcct gatcggtgtg caccaggtag ccgcgctcga ccgaccgcac cgacgtcacg
     6121 gtggtgttgg cctgcaccgg tgcagccgtc tcctttgcgt agtcggtaat gagttgcgcg
     6181 gtctccgagg ccgagaggta gccatcggga tcgtcgccgc ggtaggcata gtcgggcagc
     6241 cgggtcatcc agttcggcgt cagcaaccgc agcgagtccc aacgctgcgt gtgccatgag
     6301 ttcgccacct cgccgcgttc cagtaccaca tggtcgatgg acttttcggc gaggcagcga
     6361 ctcatcgcga gcccgcagtg gccggcgcct accacgacgg tggttgtctt aagcactttg
     6421 ggctccagtg ttttcaggcg acgtcgacga tgacgtcggt tgggttggtg atgatgtcgt
     6481 agaccgcgga gcgcttctgt gactgggcca ccagcgctcg gatctcgtcc gccgtggcat
     6541 cagcgtcgat cttgtaggtc accctgatgc cgctgaaacc gttgcggatg tcggggtcca
     6601 cgccgaggat gccctggatg tcttcgccgg cttcgatggt cgcgtgcacc gagcgcaact
     6661 gaattccacg ccgttgcgcg atcgacgcga taccggcggt caggcagccg ccgagggcga
     6721 ccagcacgta ttcgaccggc gtggcgcctt tgtcgtgccc ggcgaacgcc aacggatgat
     6781 cgacgtcata ggtaaacgga gcctcgtggt gttgctcctc accaagtcca tagaacgtgt
     6841 cgacggtgga tcggctgtgg gtgccgttga cccagtcgac ctgtgtgcgc cattggaatt
     6901 gggcgagttc tggggcatcg gctaacgcgt tgcgggcccc gagcagggcg tcgacgttca
     6961 ctccgttgtc aacggatgtg ttcagcgtgg tcatgtgttt tccttttctg tgtggaccgg
     7021 atccatcggg gaaccggcgg gttggtgtag gtgttttgac tacggtttcc gttgcggcgc
     7081 agcctcttca ggctgcatgc cgattggctg gctctacgtc acttcacctc cttcaaagct
     7141 ctttgccagc aatccattac ggactgcatg ttcgttcctg ccctctctag gcaggattga
     7201 gcggtcgtag cgcgtgcact cactcgctcg tgctcataca cgtactcccc gtactgggaa
     7261 tggatgttca ccgctccacc gccgttcggg tgaactgtga gcgcagcatc cgagaattca
     7321 tcgaagcctc tgcccgtatg ctggtcgcgc tgagcggcat gggtgcttcg cgtcggccag
     7381 tgcagatgat catgcggaaa tggtcggccc gacagccttg cccgcacatg aggcattcgc
     7441 cccaaaaatc taaaccgtgc cggcgcttcc gaagaggccg gaaatgtctt accggagcga
     7501 gctgccggtc aggccgtgcc atgtcgccta cacgctcttg gcctatatgt gttccacctg
     7561 attgcgcacc gtcttctccg aaatccaaag ggccgaagcg atctcctggc tggttcgccc
     7621 ctgtgcggag caatacctcg gcctcgcgtc gagtcaaccc cccggcccgg ggactttgcg
     7681 attttggggc aaacgcctca tgcgaccacg taagcgtcgg accgatgatc aaggctgagc
     7741 ggccagccgg gccgggaaca ctgcacaagg gatacgcgat gagcaccatc tccacgatga
     7801 ggaccgggca tcatcggcgg gcggacggat cgctggtcag ggtggatgtc gagttgggcc
     7861 gctgggtggg cagtctttac acaccggaca tgcgggtcaa ggcacgaatt gtcggcagtg
     7921 acgtcgatgt tcatgcgtgg gctattcagt cgatgaccaa cgggtgacgt tcgtgaatgt
     7981 gctccgtcga cctcgacaag cgccaggaat tcccgtggcc cgtgctcgag gaggccgccg
     8041 aacggggttt ctatagcccg ctgttctacc gcgacctgat cggcgacccg acgggtctgt
     8101 cgttgccgat gtccgggcca ccgaacgggc gatccagacc atgggcgggt ggggctacat
     8161 caccgaccat cccgtcagaa gtggtaccga gacgccaagc tgtacaccca cttcgagggc
     8221 accggcgaga tccagcgggt cgtgagctcc aacgcgctgg gtgccgccgg cgaggaaccg
     8281 ccgctgcatt tcgagctcga accgtc
//
LOCUS       DASWBF010000401         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_368218,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000401 DASWBF010000000
VERSION     DASWBF010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368218"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VF874_14930"
     CDS             complement(<1..908)
                     /locus_tag="VF874_14930"
                     /inference="COORDINATES: protein motif:HMM:NF028061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-D factor RsdA"
                     /protein_id="HEX9177310.1"
                     /translation="MPEHDPSLDAVMRTDRFVDALASGARMSPVDPLSAMLGGWRDDV
                     RSRPDTQVVTLVQASAALAKTQKPPRRNRFGLTAIGAAAAAVLCIGGFGAVVYDAGPG
                     DALYGLRSMLFGESTKTRDDAVALASQQLAQVRQLVQEGNWDEAQQRLVTLSTTVQSV
                     DDVAAQQQLVEQYNALTVQVIERNPEATLPPPGEPLPPLPSSPLTFLTVPAIPDSTST
                     STSTSTSTSPSAETPPVSTPMPTPTGPLPTPTGPLLTPTGPLLTPTGPLLTPTGPLPT
                     PTGPLLTPTGPLPTPTGPLLTPTGPLP"
     gene            complement(901..1473)
                     /locus_tag="VF874_14935"
     CDS             complement(901..1473)
                     /locus_tag="VF874_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9177311.1"
                     /translation="MTNSGDRLDAVVADAVGGDRGALREVLETVRPIVVRYCRARLGT
                     AERSGLSADDVAQEVCLAAITALPRYRDQGRPFLAFVYGIAAHKVADAHRAAGRNRAE
                     PTEVIPERATFEAGPEQMALDAESSARMNALLQILPEKQREILILRVVVGLSAEETAE
                     AVGSSPGAVRVAQHRALTRLREEMIGAGYA"
     gene            complement(1556..2338)
                     /locus_tag="VF874_14940"
     CDS             complement(1556..2338)
                     /locus_tag="VF874_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177312.1"
                     /translation="MSAALLAAAFGDDPGRWPLPAAASPHDRWLRAVAAGGQGRYGSA
                     TADLATLRREVHSGPVASLGHSTQASFLRQLGGHARARPWDGRALALAASDPEACVDA
                     LLGLAADALGMGRFAASAALLDRAKEPLDGSPAPARLPVRWQWVVAELAMARGDGTEA
                     VRHAEQAVELAAGCPSARHRVKTQVVYAAALCSAGRIDEARAVADDALSATATLGLVP
                     LQWALASLLVDIVGDPARADQLTAIRDACAQAVRRAGGVWCR"
     gene            2691..2981
                     /locus_tag="VF874_14945"
     CDS             2691..2981
                     /locus_tag="VF874_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9177313.1"
                     /translation="MPQPQQLPGPNADIWDWQIQGLCRGVDSSMFFHPDGERGRARAQ
                     REMRAKEMCRRCPVIAQCRAHALSVGEPYGIWGGLSEAERELLLKRGIRRTA"
BASE COUNT          499 a         1072 c         1016 g          480 t
ORIGIN      
        1 ggcagcggac ccgtcggcgt cagcaacgga ccggtcggcg tcggcaacgg accggtcggc
       61 gtcagcaacg gaccggtcgg cgtcggtagc ggacccgtcg gcgtcagcaa cggaccggtc
      121 ggcgtcagca acggaccggt cggcgtcagc aacggaccgg tcggcgtcgg tagcggaccc
      181 gtcggcgtcg gcatcggcgt cgacaccggc ggcgtttcgg cagacggtga tgtcgatgtc
      241 gatgtcgatg tcgatgtcga tgtcgagtcc gggatcgcag gcaccgtcag gaaagtcaac
      301 ggcgacgacg gcagcggcgg aagcggctct cccggcggcg gcagcgtggc ctcgggattg
      361 cgctcaatca cctggacggt caacgcgttg tattgctcga ccaactgctg ctgggccgcg
      421 acgtcgtcga cgctctgcac ggtggtggac agcgtgacga gcctctgctg cgcctcgtcc
      481 cagttgccct cttgaaccag ctgacgcacc tgggccaatt gctgtgaagc cagcgccacc
      541 gcgtcgtcgc gcgttttcgt cgactcgccg aacagcatgc tgcgcaaccc gtagagcgca
      601 tcgcccggcc ctgcgtcgta cacgacggcg ccgaagccgc cgatgcacag caccgcagca
      661 gccgctgcac cgatcgcggt caacccaaac ctgttgcggc gcggcggttt ctgtgtcttg
      721 gccagcgcgg cagacgcctg taccagggtc accacttggg tgtcggggcg gctgcgcacg
      781 tcgtcacgcc aaccgcccag catcgccgac aaaggatcca ccggcgacat ccgagcgccg
      841 gaggcgagcg catcgacgaa acggtcggtg cgcatgacgg cgtcgaggct ggggtcgtgc
      901 tcaggcatag ccggccccga tcatctcttc gcgcagccgc gtcaacgcgc gatgctgcgc
      961 gactcgcacc gcacccggtg agctgccgac tgcttcggcg gtctcctccg cagaaagccc
     1021 cacgaccaca cgcaggatca ggatttcgcg ctgcttctcc ggcagaatct gcagaagtgc
     1081 gttcatccgg gccgacgatt cggcgtcgag ggccatctgc tcgggcccgg cctcgaacgt
     1141 ggctcgttcg gggatgacct cggtgggctc ggcacggttg cgcccggccg cacggtgggc
     1201 gtctgccacc ttgtgggccg cgatgccgta cacgaacgcc aagaagggcc tcccctggtc
     1261 cctgtagcga ggcaacgccg tgatggcggc caagcacacc tcctgagcca catcgtctgc
     1321 cgaaagaccg ctgcgctccg ccgttccgag cctcgcgcgg caataccgaa cgacgatcgg
     1381 acgaacggtc tccagcactt cgcggagtgc gccacgatcc ccccccactg cgtcagcaac
     1441 gacagcatcg aggcgatctc ccgaatttgt catcgacgac ggcgtctcca acgttacgag
     1501 ccgagcgctt ctcggcgacg taacgacaat aacgatccaa caacgttgtt cgggttcagc
     1561 ggcaccaaac gccaccggcg cggcgcactg cctgcgcgca cgcgtcgcga atcgcggtca
     1621 attgatctgc tcgggcggga tcgccgacga tgtcgacgag caaactggcc agcgcccatt
     1681 gcagcggaac cagccccaac gtggccgtcg ccgacagcgc atcgtcggcg acggcgcgcg
     1741 cttcgtcgat ccggccggcg ctgcacagtg cggcggcgta caccacctgc gttttcaccc
     1801 ggtgccgtgc cgacgggcac cccgcggcca gctcgactgc ctgctcggca tgccgaaccg
     1861 cctcggtgcc gtcgccgcgg gccatcgcga gctcggcgac cacccattgc cagcgcaccg
     1921 gcaaccgagc cggcgccggc gatccgtcca gcggctcttt ggcgcggtct agcagggccg
     1981 ccgacgccgc gaaccgcccc atgcccaacg cgtcggcggc cagcccgagc agggcgtcga
     2041 cgcaggcttc ggggtccgat gccgccagcg cgagcgcccg gccgtcccag ggccgcgccc
     2101 gcgcgtggcc gcccagctgc cggagaaacg aggcctgcgt gctgtgcccc agcgatgcca
     2161 ccgggccgct gtgcacctcg cgccgcagcg tcgccagatc ggccgtcgcg ctgccatagc
     2221 gaccctggcc gcccgccgcg acagcccgca accaccggtc atgaggtgag gcggccgccg
     2281 gcaaaggcca ccgccccgga tcgtcgccga acgccgctgc gagcaacgcg gcgctcatcg
     2341 acggttaaca gtttgtgctg tggacgttaa cgccatgtca cctgcttacc gggtccgagg
     2401 aattcgggag ccacaccacc gtcggcccac gtgcagaaac ccgttggcgg ccattgctgc
     2461 gcggttctga atagcacggt gttcgttgcc gatgggtcgc aacgatgaac gtgatgtaaa
     2521 ttctcggcct gcggttatgc ggttacgcac agtaggtagc tattgacggg ctttcgtaag
     2581 cacccctacc ttgtcagaac gagtagtcat cggcgacatg tctcggatcg ttccgcactc
     2641 accgtctgat cgggccgtgc ccaattggac tcggcgagag gggttttccc atgccacagc
     2701 cgcagcagct acccgggcca aacgccgaca tctgggattg gcagatacag ggcctgtgcc
     2761 gaggtgtcga ttcgtcgatg ttctttcacc ccgacggcga acgcggccgt gcccgggcgc
     2821 aacgggagat gcgggccaag gaaatgtgcc gccgctgccc ggtgatcgcc cagtgccgcg
     2881 cgcacgccct gtccgtcggc gagccgtacg gcatctgggg cgggctctcg gaagctgagc
     2941 gcgaactgct gttgaagcgg ggcatccgcc gcaccgccta gccgctctta gcctgaatcc
     3001 gtggatcagt gatttcgtgc cgtcgcgggg ggctgatttc tgcacgattg ggcggttcgg
     3061 aatggtg
//
LOCUS       DASWBF010000402         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_46716, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000402 DASWBF010000000
VERSION     DASWBF010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46716"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2046
                     /locus_tag="VF874_14950"
     CDS             <1..2046
                     /locus_tag="VF874_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9177314.1"
                     /translation="VTAPDGVYGPAARAAGADIAEYLNNAPHVTGVVSAWTAPPPGAG
                     SLIGVDGNSGLVVAGVSGGEKEAPQRAQVMADAVTESALRAHRDVTVLAGGSAMVFAQ
                     INSQTGRDLLVMETIAVPIIFAVLVWIFGGLLAAALPVVVGGLAIVGSLAILRLIALA
                     SDVSIFALNLSTALGLALAIDYTLLIVSRYRDEIAGGAQRDEALTKTMLTAGRTVLFS
                     ATTVALSLAAMVLFPMYFLKSFAYAGIATVALCAVAALVVTPAAIVVLGARLDSLDVR
                     RGIRRLGGRDEPPPTPVRQLFWYRWTTSVMRRAVPIGLAGSAVLVVAGLPFLGITWGF
                     PDDRVLPGTASAHQVGDRMREGFSDDSASAVTIVIPDATGLTEPDFEQYAARLSAVVD
                     VGAVSAPTGTFTDGTRIGAAAAAAGIADGSAYLTVNSSAPLFSPASEAQLDRLREVPT
                     PGGRPVEFTGMAQINRDSVAAITSRVPLVLGVIAVVTFALLFLLTGSLVLPIKALVLN
                     VLSLTAAFGALVWIFQDGHLGAFGTTPTGTLVATMPVLLFCIAFGLSMDYEVFLIARI
                     REYWLASQRTRADIDDSVALGLARTGRVITAAALVMSISFAALIAAQVSFMRMFGVGL
                     TLAILVDATLVRLILVPAFMRILGRWNWWAPAPLARLHRHIGVSKSAPGHRKSRSRA"
BASE COUNT          276 a          666 c          716 g          397 t
ORIGIN      
        1 gtcactgcgc ccgacggagt gtatggccca gccgcccgcg cggcgggcgc ggacatcgcc
       61 gaatacctca acaacgcgcc gcatgtgacg ggggtggtct cggcctggac ggcaccgccg
      121 ccgggcgcgg gcagcctcat cggcgtcgac ggaaactcgg ggctcgtggt ggcgggggtt
      181 tccgggggtg agaaagaggc gccccagcgc gcacaggtaa tggccgacgc ggtcacggaa
      241 tcggcgctgc gtgcacatcg cgacgtgacc gtgctggccg gcgggtcggc aatggtgttc
      301 gcgcagatca actctcagac cggacgcgac ctgttggtga tggagacgat tgcggttccc
      361 atcattttcg cggtgctggt gtggatcttc ggtggtctgc tggccgcggc gctgccggtg
      421 gtggtgggcg ggttggcgat tgtcggctca ctggcgatcc tgcgcttgat cgcgctcgcc
      481 agcgacgtgt cgatcttcgc cctgaacctg tcgactgccc tgggcctggc gctggccatc
      541 gactacacgt tgttgatcgt cagccgatac cgcgatgaga tcgccggagg tgcgcaacgc
      601 gatgaggcgc tgaccaagac gatgctcacc gcggggcgca ccgtgttgtt ctcagcgacg
      661 acggtggcgc tgtcattggc cgcaatggtg cttttcccga tgtacttcct caagtcgttc
      721 gcctatgccg gcatcgcgac ggtggcgctg tgcgcggtgg ccgcgctggt cgtcacgccg
      781 gcggcgatcg tagtgctcgg cgcccgcctg gactcgttgg atgtgcgccg tggcatccgg
      841 cggctgggtg gccgcgacga accgccgccc acgccggtgc ggcagctctt ctggtaccga
      901 tggacgacct cggtgatgcg gcgtgcggtg cccatcggtc tggcgggctc cgcggtgttg
      961 gtcgtggccg gcctaccgtt cctggggatc acctggggtt tccccgacga tcgcgtgctc
     1021 ccggggacgg cgtcggccca tcaggtcggt gaccggatgc gtgagggctt cagcgacgac
     1081 tccgcgagcg cggtaaccat cgtgataccc gatgcgacgg gcctgaccga acccgatttc
     1141 gagcaatatg ccgcgcggct gtctgcggtc gtcgacgtcg gggcggtcag cgcgcccacc
     1201 ggcaccttca ccgacgggac gaggatcggt gcggctgcgg ccgccgcggg gatcgccgac
     1261 ggcagcgcgt acctgacggt gaacagcagc gcaccgttgt tctcgccggc ttccgaggcc
     1321 cagctcgacc ggctgcgcga ggttcccacc ccgggggggc gtcccgtcga gttcaccggc
     1381 atggcccaga tcaaccgcga cagcgtcgcg gcgatcacgt cgcgcgtgcc gttggtcctg
     1441 ggcgtcatcg ccgtcgtcac gtttgcgctg ctattcctgc tgacgggcag cctcgttctc
     1501 ccgatcaagg cgcttgtact caacgttctt tctctgaccg cggcatttgg cgcgctggtg
     1561 tggatctttc aggacggcca tctgggcgca ttcggaacca cacccaccgg cacgctcgtc
     1621 gccaccatgc ccgtcctctt gttctgcatc gcgttcggac tgtcgatgga ctacgaggtc
     1681 ttcctgatcg cccgtatccg cgagtattgg ctggcaagcc aacgcacccg cgccgacatc
     1741 gatgacagcg tggccctcgg gctggcccgc accgggcggg tcatcacggc cgccgcattg
     1801 gtgatgtcga tctcctttgc ggccctgatc gccgcgcaag tgtcgttcat gcggatgttc
     1861 ggggttggtc tgacactggc gattctggtt gacgccaccc tggtccggct cattctggtg
     1921 ccggccttca tgcgaattct cggacggtgg aactggtggg cgcccgcgcc tctggcgcgg
     1981 ctgcaccgac acatcggtgt cagcaaaagc gctcctggtc accgaaagtc caggtcaagg
     2041 gcttgaggaa atttt
//
LOCUS       DASWBF010000403         4473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_299733,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000403 DASWBF010000000
VERSION     DASWBF010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299733"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VF874_14955"
     CDS             <1..246
                     /locus_tag="VF874_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177315.1"
                     /translation="SGSRQPGDIVTVTYLDEAGMPRTDFNVTLPWTKTVISGGNVLLN
                     SVTAVSLASHLNCTITDANGQAVASQDYNTIATTCNR"
     gene            complement(254..937)
                     /locus_tag="VF874_14960"
     CDS             complement(254..937)
                     /locus_tag="VF874_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HEX9177316.1"
                     /translation="MSSTQPAAASASRVSPDHPGVNKLFALLAYGEVAAFYRLTDQAR
                     MSPSLRGRIAMASMAAAEMGHYEMLRDALAARGVDVEQVMTGYISALENYHRLTTPST
                     WLEALVKTFIGDALAADFYREIADVLPDEVADVVHGVLSETGHSQFVVAELRAAVAAS
                     GKQRNRLTLWARRLLGEAITQAQYVLAEHDELADLVVSGTGGLGKITDFFDRLQRTHA
                     ERMAELGLA"
     gene            1147..2598
                     /locus_tag="VF874_14965"
     CDS             1147..2598
                     /locus_tag="VF874_14965"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9177317.1"
                     /translation="MTALNPTFAKLGVRDEIVRALAENGIEHPFAIQELTLPLALVGD
                     DLIGQARTGMGKTLAFGVPLLHRITGDPERPLTGAPRALVVVPTRELCLQVHGDLTNA
                     AKYLTAGDRPLSVVSIYGGRPYEPQIEALQTGAEVVVGTPGRLLDLAQQGHLQLGGLS
                     MLVLDEADEMLDLGFLPDIERILRQIPTDRQAMLFSATMPDPIITLARTFMNQPTHIR
                     AEAPHSSAVHATTEQFVYRAHAMDKIELVSRILQASGRGATMVFTRTKRTAQKVADDL
                     AERGFKVGAVHGDLAQVAREKALKAFRTGSIDVLVATGVAARGIDIEDVTHVINYQCP
                     EDDQAYVHRIGRTGRAGRAGVAVTLVDWDELPRWSTIDKALNLDCPAPAETYSSSPHL
                     YTELDIPTDVTGRVGSAQKPKRREPTQSRDGADKPASGRTRSRQRTRGGKAATGHAKK
                     AEKAPTDQETADSGHSVRRRRRRRPRQAATSAG"
     gene            2607..3824
                     /locus_tag="VF874_14970"
     CDS             2607..3824
                     /locus_tag="VF874_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177318.1"
                     /translation="MVKPERRTKGDILASVAIVVVVAVVGVLIWWNSDARATISRPAP
                     APAATITAARAVPANLRQLWTAPSPRTDEPVIVASTVVTGDGHEMVGRDPNTGQQRWT
                     FARDRDLCAVTWVYDLAVAAYPDSRGCGQISTVEARTGDRGPTRSSYADKQITLSTDG
                     TTVLAAGRTRIEMWRSDMVRTLAYGDIDAPINPPVPPDPPCQFLSTAASSSAASVLET
                     CSSFPDVRLTLLKVSKEDVEPEEKYVQQPGLKPDSAARVLAVSDLRTAVYLPIPQPRV
                     VVYDETGHETASTLLPRQPGPDIAVSRAGDVITVWTGDSVSVLDGSSLAFRYTIAAGS
                     AVPLGPGEMMADRLLIPVTGGIGVYEPKTGTFERMIPVDRGNVGGPIVPGVVGTTVVE
                     QRGGTVVALGEAP"
     gene            complement(3832..>4473)
                     /locus_tag="VF874_14975"
     CDS             complement(3832..>4473)
                     /locus_tag="VF874_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9177319.1"
                     /translation="DPDKLPVSVHEVLLGEVEPSAALVDTSEGMTLLPANIDLAGVEA
                     MLLMRAGREYALKRALAKVKDEFDAVIIDCPPSLGVLTLNGLTAADEVIVPLQCETLA
                     HRGVGQFLRTIADVQQITNSDLKLLGALPTLYDSRTTHTRDVLLDVADRYDMPVLSPP
                     IPRTVRFAEASASGCSVLAGRKNKGSAAYRDLASALLKHWKSGKPLASFTPEV"
BASE COUNT          751 a         1586 c         1485 g          651 t
ORIGIN      
        1 tcaggcagcc gccagccggg cgacatcgtc acggtgacgt acctcgacga agcggggatg
       61 ccgcgaacag acttcaacgt cacgctgccc tggacgaaga ccgtcatctc cgggggaaac
      121 gtgctgctga actccgtcac agccgtgagc ctcgcgagcc acctgaactg cacgatcacc
      181 gacgcgaacg ggcaggcggt cgcgtcgcag gactacaaca cgatcgcgac gacgtgtaac
      241 cgctgacgcg gagctacgcc aggccgagct cggccatccg ctcggcgtgg gtgcgctgca
      301 gccggtcgaa gaagtcggtg atcttgccga gcccgccggt gcccgacacc accaggtcgg
      361 ccagttcgtc gtgttcggcc agcacgtact gggcctgcgt gatcgcctca ccgagcagcc
      421 gacgcgccca cagcgtcagg cggttgcgct gtttgccgct ggccgccacc gcggcgcgca
      481 gctcggcgac cacgaactgc gaatgcccgg tttccgagag cacaccgtgc accacgtcgg
      541 cgacctcgtc gggcagcacg tcggcgatct cacggtagaa gtcggcagcc agcgcgtcgc
      601 cgatgaaggt cttcaccaac gcctccagcc aggtgctcgg cgtggtcagc cggtgatagt
      661 tctccagcgc agagatgtag ccggtcatca cctgttcgac gtcaactccc ctggcggcca
      721 acgcatctcg cagcatttcg tagtggccca tctcggcggc cgccatgctg gccatcgcga
      781 tccggccgcg caggctgggc gacattcgcg cctggtcggt gagccggtag aacgcagcca
      841 cttcgccgta tgccagcagc gcgaacagct tgttgacgcc cggatggtcg gggctgacgc
      901 gggaggcgga cgcagccgcc ggctgcgtgg aactcatggc cttcagccta gcccggtatg
      961 atagacgtcg tggcggcgcg aagccgccgc acctgtatat gtgcgtgcac gcagttggcc
     1021 ccgctccgag gagcgcaggg ctcacttgaa tcggctgcga atcggcgtcg acctgacgcc
     1081 ggccagacgt gtcttttcgg actcgtgcgc gcgtggcgac gagaatgacg agacacgaaa
     1141 ggccacatga ccgccctcaa tccaacattt gccaaactcg gcgttcgcga cgaaatagtc
     1201 cgcgcattag ccgagaacgg cattgaacat ccctttgcta tccaagagct gaccctgccg
     1261 ctggcgctgg tcggcgacga cctgatcggc caggcccgca caggaatggg caagacgctc
     1321 gcgttcggtg tgccgctgct gcaccgcatc accggcgacc ccgagcgccc gctgaccggc
     1381 gctccccggg cgctggtggt cgtccccacc cgcgagttgt gccttcaggt gcacggcgac
     1441 ttgaccaacg ccgccaagta cctgaccgcc ggagaccgcc cgctgagtgt cgtatcgatc
     1501 tacggcggcc gtccgtatga gccgcagatc gaggcgctgc agaccggtgc cgaggtggtc
     1561 gtgggaacgc cgggccggct cctggacctg gcccagcagg gccacctgca gctcggcggg
     1621 ctgtcgatgc tggtgctcga cgaggccgac gagatgcttg acctcgggtt cctgcccgac
     1681 atcgagcgca tcctgcgcca gatccccacc gaccggcagg cgatgctgtt ctcggcgacg
     1741 atgcccgacc cgatcatcac gctggcccgc acgttcatga accagcccac gcacatccgg
     1801 gccgaggcgc cgcactcctc tgcggtccac gccaccaccg agcagttcgt ctaccgcgcg
     1861 cacgcgatgg acaagatcga actggtcagc cgaatccttc aggccagcgg ccgcggcgcg
     1921 acgatggtct tcacccgcac caagcgcacc gcccagaagg tggccgacga cctcgccgaa
     1981 cgcggcttca aggtcggcgc agtacacggc gaccttgcgc aggtcgcccg ggagaaggcg
     2041 ctgaaggcat tccgcaccgg cagcatcgac gttctggtgg ccaccggcgt ggccgcccgc
     2101 ggcatcgaca tcgaggacgt cacccacgtc atcaactacc agtgccccga agacgatcag
     2161 gcatacgtgc atcgcatcgg ccgcaccggg cgcgcggggc gcgcgggtgt ggcggtcacg
     2221 ctggtggact gggacgaact gccccgctgg tcgacgatcg acaaagcact gaatctcgac
     2281 tgtcccgctc cggccgagac gtactccagc tcgccgcatc tgtacaccga actggacatc
     2341 cccaccgacg tcaccggtcg agtcggtagc gcgcagaagc ccaagcgccg ggagccgact
     2401 caaagtcgcg acggcgccga caagcccgca tccggccgca ctcgctcccg ccagcgcacc
     2461 cgcggcggca aggctgcgac cggtcacgcc aaaaaggccg agaaggctcc cactgaccag
     2521 gagaccgccg actccggcca ctcggttcgg cgtcgtcggc gccgccgtcc acgtcaggcc
     2581 gcgacaagcg ccggttagcc accgcattgg tcaaacccga acgccgcacc aagggcgaca
     2641 tcttggcgtc cgtggccatc gtggtcgtcg tcgcagtggt gggggtgctg atctggtgga
     2701 acagcgacgc gcgcgccacg atcagccggc cggcgcccgc accggcggcg acgatcaccg
     2761 cggcgcgggc ggtaccggcg aacctccgcc agctgtggac cgcgccgagc cctcggaccg
     2821 acgagccggt gatcgtcgcc agcaccgtcg tcaccggcga cggacacgag atggtgggcc
     2881 gggatcccaa caccgggcag cagcgctgga cctttgcccg cgaccgcgac ctgtgcgcgg
     2941 tgacctgggt gtacgacctg gcggtggccg cctatcccga cagccgcggc tgcggccaga
     3001 tcagcacggt cgaggcgcgc accggtgacc gcgggcccac ccgctcctcg tacgccgaca
     3061 agcagatcac gctgtccact gacggcacca cggtgctggc cgccgggcgc acccgcatcg
     3121 agatgtggcg ctccgacatg gtgcgcacgc tggcctacgg cgacatcgac gccccgatca
     3181 acccgccggt accgcccgac ccgccgtgcc agtttctgtc gacggcggcc agctcgtcgg
     3241 ccgcctcggt cctggagacc tgcagcagct tccccgacgt ccggctgact ctgctcaagg
     3301 tctccaagga agacgtcgaa ccggaagaga aatacgtcca gcagccggga ctgaagcccg
     3361 attccgccgc gagggtgctc gcggtctccg acctgcgcac cgcggtgtac ctgccgatac
     3421 cgcaaccgcg ggtcgtggtc tacgacgaga ccggccatga gacggccagc acgcttttgc
     3481 ccaggcaacc tgggcccgac atcgcagtgt cgcgtgccgg tgatgtgatc accgtctgga
     3541 ccggcgacag cgtctcggtg ctcgacggca gcagcctggc gtttcgctac acgatcgcgg
     3601 ctggcagcgc ggtgccgctg ggaccaggcg agatgatggc cgaccggctg ctgatcccgg
     3661 tcaccggcgg cattggggtg tacgagccga agaccggcac cttcgagcgg atgattcccg
     3721 tcgaccgcgg caacgtcggc gggccgatcg tgccgggcgt cgtcggcacc accgtcgtcg
     3781 agcagcgcgg tggcacggtc gtcgcgctcg gtgaagcgcc gtagcgaccg atcagacctc
     3841 aggggtgaag ctggccagcg gcttgccgga cttccaatgc ttgagcagcg ctgaggccag
     3901 gtcgcgatag gcggcggacc ccttgttctt gcggccggcc agcactgagc accccgacgc
     3961 gctggcctcc gcgaaccgca ctgtgcgcgg tatcggcggc gacagcaccg gcatgtcgta
     4021 gcggtcggcg acgtcgagca ggacatcacg agtgtgggtg gtgcgagagt cgtacagcgt
     4081 gggcagcgca cccagcagct tcaggtcgga gttggtgatc tgctggacgt ccgcgatggt
     4141 ccggaggaac tgcccgacgc cgcggtgcgc cagggtttcg cactgcagcg gcacgatcac
     4201 ctcgtcggcg gcggtcaggc cgttgagcgt cagcacgccc agcgacggcg ggcagtcgat
     4261 gatcaccgcg tcgaactcgt ctttgacctt ggccagcgcg cgtttgagcg cgtactcgcg
     4321 accggcccgc atcagcagca tcgcctcgac gccggccaag tcgatgttgg cgggcagcag
     4381 cgtcatgccc tcgctggtgt ccaccagcgc cgccgacggt tccacctcgc cgagcagcac
     4441 ctcgtgcacc gacaccggca gcttgtcggg gtc
//
LOCUS       DASWBF010000404         5866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1024093,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000404 DASWBF010000000
VERSION     DASWBF010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024093"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF874_14980"
     CDS             <1..230
                     /locus_tag="VF874_14980"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9177320.1"
                     /translation="LANRRLWADVGRHSPDGICLDAAGAAWYADVGNRCCVRVGEGGE
                     VLNRVQLDPRAVASLEGQSPRCTKVIGVSNR"
     gene            complement(355..2040)
                     /locus_tag="VF874_14985"
     CDS             complement(355..2040)
                     /locus_tag="VF874_14985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX9177321.1"
                     /translation="MGGPGGVGDSASTPRAAPAAAHLGAAPCLHCRNGPAPERERGFA
                     GTVTTFRGNFDSWPFCQGESSMTELNASTLSQLPSKVAAPSYDRSRLKRGIAHFGVGN
                     FHRAHQAFYIDRCLALPGQEDWGIVGIGLFGGERGRQKAAQFRTQDCLYSLTIAPPKG
                     EPGVRVIGAQLDYLLAPEEPDEVLALLTSPDLRIVTLTITEGGYHVDPHSNAFVTDHP
                     DVAHDLAGNGAPRTVFGFVTEALARRRAAGIKPFTVASCDNLRHNGDVARTAFVGFAS
                     ARDPELGAWIQASVAFPNSLVDRITPSVTAEDAARLNAASGLDDRIPLVAEDFTQWVI
                     EDRFTDGRPALEKVGVQLSDEVKLWEQVKVRVLNASHLTLAYPALLLGYHEVAEAMRD
                     PQVPVLLERFLDKIVLPLLEAPRNVNLVDYKNTVLERFSNEAMHDQLTRIASDSASKV
                     PVYFTTTLKEMLAQGGDHRIPAFTLAAWSRVLQGQDDDGKAFEILEPRLDDRARRQLA
                     SGDPAQALRIEPLLASGVAEHADFAASFEGYRKALAEHGARATLQAILDATAG"
     gene            2326..3588
                     /locus_tag="VF874_14990"
     CDS             2326..3588
                     /locus_tag="VF874_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177322.1"
                     /translation="MGALPALEPRASVKWLRRFARTTPGVVSMIAVAVAACCMIAAAV
                     CAAQLNGRIGRQHAVLDRSEPLAYSAQKLYAALSAADAAAASAFLSGGIQTAPMRARY
                     QQALADAASALADATAGVTDFDTRTAAAEMSAQLSAYTGLVESARANSRQGFPVGSGY
                     LREASSLMQTALLPGAERIYAGNLAKVDEDQRAVGSVPTVGLVLMALVLATIGVGSVI
                     VFARTNRRFNIGLVVAAAAALLVIGWIIVATRLAAGDIEHSRAAGTARFEQLAKARIL
                     AEQARTDETLQLIAHGDITAGEASFNGHVGDLGKLVGTGPSAAADGVAKWTASHRRQV
                     EAYLRGDYPAAVAQAIGPDPGASAAHFAVVESGLRGEIEKTRATLRDRVSAAGGRLAW
                     SPTGTLVLMVIAAAAAVVGLWPRLKEFV"
     gene            3813..4547
                     /locus_tag="VF874_14995"
     CDS             3813..4547
                     /locus_tag="VF874_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9177323.1"
                     /translation="MAAIAERGRLVVGVDQNTYPFGFRNPSSGQLEGFDIDLAREVAR
                     RIFGDPDRIDLRVVDASQRESALQSGQVDVVVRTYSITCDRKRSVEFSTVYYYANQRI
                     LAAKGSGIDSAAALSGKRVCAVRGTTSLSAMFALNPRPTLFSVTTWTDCLVMVQQGQI
                     DAISTDDVVLFGLAAQDPTVEVVGPSIAVEPYGVGIKKENIDLVRFVNGALDQMRADG
                     TWERLYEAHLRSLGPSPGPPPARYGD"
     gene            4562..>5866
                     /locus_tag="VF874_15000"
     CDS             4562..>5866
                     /locus_tag="VF874_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX9177324.1"
                     /translation="MTCADPVCAGTVVGGYCDLCGAASATSAPQGPSMATAWSGSNAR
                     SVQTARIRSSKSASARGRLGAGIVAIPRIPTVDPAAAIMTDPQVPEPNRFCGNPECNK
                     PVGRGRDGNPGRPEGFCTQCGTRYSFVPKLSRGDLVGGQYEVQGCIAHGGLGWIYLAI
                     DRNVHNRWVVLKGLLNSGDANAMAVAAAEVLALAEVEHPNIVRIYNFVEHVDAAGVPV
                     GYIVMEYVGGRSLKQIRKARNAPLPPDQAVAYIVEIASALHYLHTQGLAYCDFKPDNV
                     MQTDEQLKLIDLGAVVAMDDEESAIYGTRGYQAPEIAKTGPTVASDVYAVGRTLAVLV
                     MDVPQEHGHFVEQLPGPATMPVLAKHESLYRAILRATDPDPGRRFSSMAEMADQLTGV
                     LHEIAAADSDIAQPRMSNHFSPQPAVYGAGRDAPVEPADVIAA"
BASE COUNT          960 a         1913 c         1996 g          997 t
ORIGIN      
        1 ccctggcgaa tcggcgtctg tgggcggacg tcggccgcca ctcacccgac ggaatctgcc
       61 tcgacgctgc cggcgcggcc tggtacgccg acgtcggcaa ccggtgctgc gtacgcgtcg
      121 gcgagggcgg cgaggtcctg aatcgggtgc aactcgaccc ccgggcggtc gccagcttgg
      181 aggggcaatc cccacgatgc acgaaagtca ttggcgtatc gaatcggtag cgtttgcaaa
      241 gttccccgct gtcgaaccac aggtaccgag aacgctccgg ttggcaccct tcccgttgag
      301 cgccagtagg gcgcgacgcc gaccggctta tgtcggggtc atcgccgcct cgcgtcagcc
      361 tgcagtggcg tcgagtatcg cctgcagtgt cgctctcgcg ccgtgctcgg ccagagcctt
      421 gcggtagccc tcgaagctgg cggcgaagtc cgcgtgctcg gcgacaccgc tggctagcag
      481 cggctcgatc cgcagcgctt gggccggatc gcccgatgcc agttgccggc gggctcgatc
      541 gtcgaggcgc ggctcgagta tctcgaaggc tttgccgtcg tcgtcctggc cctgcagcac
      601 gcggctccag gcggcaaggg taaatgcggg gatgcggtgg tcgccgccct gcgcgagcat
      661 ctccttgagc gtggtggtga aatacaccgg caccttggac gcgctgtccg aggcgatgcg
      721 cgttagttgg tcgtgcatcg cctcgttgga gaagcgttcg agcacagtgt tcttgtagtc
      781 cacaaggttt acgtttcgcg gcgcctcaag cagcgggagc acgatcttgt cgaggaagcg
      841 ttccagtagc accggcacct gcgggtcgcg catcgcttcg gccacctcgt ggtagcccag
      901 cagcagggct gggtaagcca gggtgaggtg gctggcgttg agcacgcgta ccttcacctg
      961 ctcccacagt ttcacttcgt cgctgagttg tacgccgact ttttcgagtg cggggcggcc
     1021 gtcggtgaag cggtcctcga tcacccactg ggtgaagtcc tcggccacca gcggaatgcg
     1081 gtcgtccagg ccgctggccg cgttgaggcg cgcggcgtct tccgcagtta ctgagggcgt
     1141 gatgcggtcg accagcgagt tgggaaaggc gacgctggct tggatccagg cgccgagttc
     1201 tgggtcgcgc gcgctggcga agccgacgaa ggcagtgcgc gccacgtcgc cgttgtgtcg
     1261 caggttgtcg cacgacgcca cggtgaacgg cttgatgccg gccgcgcgtc gccgcgccaa
     1321 ggcctcggtg acgaagccga acaccgtgcg cggcgcgccg ttgccggcga ggtcgtgggc
     1381 cacgtccggg tggtcggtga cgaaagcgtt cgagtgcgga tcgacgtgat agccgccctc
     1441 ggtgatggtc agcgtgacga tgcgaaggtc cgggctggtc aacagcgcga gcacctcgtc
     1501 gggctcctcc ggtgcgagca ggtagtcgag ctgggcgccg atcacgcgca cgccaggttc
     1561 acccttcggc ggcgcgatgg tgagcgaata caggcaatcc tgcgtgcgga actgcgcggc
     1621 cttctgccgg ccccgctcac ctccgaacag gccgatgccg acgatgcccc aatcctcctg
     1681 gccgggcagg gcgagacagc ggtcgatgta gaaggcctga tgcgcgcgat ggaaattgcc
     1741 cacgccgaaa tgcgcgatgc cgcgtttgag ccggctgcgg tcgtaggacg gcgccgctac
     1801 cttggacggc aactgcgaca gcgtgctggc gttgagttcg gtcatgctcg actctccttg
     1861 acaaaaaggc caactgtcga agttaccccg aaacgtcgtt acggttcccg caaagccccg
     1921 ttcgcgctcg ggcgccggtc cgttccggca gtgcaggcaa ggcgcggcgc cgaggtgcgc
     1981 tgcggccggc gctgcgcgag gtgtgctagc gctgtcgccc acgccgcccg gtccgcccaa
     2041 gcgctggggt gacgccgggg agctacacgc tgatctcccg aaggaccacg agaacaacag
     2101 cgttgatctc ttcgggtggt gtaacgaaat cacatcgcag ttagacaacg atcggcccgt
     2161 gactcgccga agggtcaccc ggaccccgct gcgtccacat cacaaaacct gaggtcaagg
     2221 ccgcgagaaa cggatcgact caagatcacc gtcgaatcga tcaatgccgg ttaccgcccc
     2281 acctgttgcg gttaccatcc cgttgagggg cagggggtga acgatatggg cgctctgccc
     2341 gcgctggagc cgcgggcttc tgtgaagtgg ctgcgccgtt ttgcccggac gacgcctggt
     2401 gtcgtcagca tgatcgccgt cgccgtcgcg gcatgctgca tgattgccgc agccgtatgt
     2461 gcggctcagc tcaacgggcg gatcggcagg cagcacgcgg ttctggaccg ttccgagccg
     2521 ctcgcctact cggcgcagaa actgtacgcg gctctgtcgg ccgccgacgc cgccgcagca
     2581 tcagcgttcc tctcgggcgg gatccaaacg gcaccgatgc gggcgcgcta tcagcaagcc
     2641 ttggcagacg cggcatcggc actggcggac gcgacagcgg gggtcaccga tttcgatacc
     2701 cgcaccgcgg cagcggaaat gtcggctcaa ctatctgcgt acacggggct ggtcgagtcg
     2761 gcgcgggcga acagccgtca ggggttcccg gtcggatccg gatacttgcg ggaagcgtcg
     2821 tcgttgatgc agaccgcgct ccttcccggc gccgagagga tctacgccgg caatcttgcc
     2881 aaggtcgacg aagaccagcg cgccgtcggt tctgtgccga cggtcggtct cgtgctgatg
     2941 gcgcttgtcc tggcgacgat cggcgtcggc tcggtgatcg tgtttgcccg cacgaaccga
     3001 cggttcaaca tcgggctcgt ggtcgcggcg gccgcggcgc tgctggtgat cgggtggatc
     3061 atagtggcta cccgcttggc ggcaggcgac atcgagcaca gtcgagctgc cggcaccgcg
     3121 aggttcgagc agctcgcgaa ggctcgcatc ctggccgagc aggcccggac cgatgagacg
     3181 ctgcagctga tcgcgcatgg cgacatcact gccggcgaag cgtcgttcaa tggccacgtc
     3241 ggtgacctgg gcaaactggt cggaaccgga ccgtcggccg cggcggacgg tgttgcgaaa
     3301 tggacagcca gccaccgccg gcaggtggag gcgtacctcc gcggcgacta ccctgccgcg
     3361 gtcgcgcagg cgatcggccc cgatccgggc gcttcggcag cgcacttcgc cgtcgtcgaa
     3421 tccggcttgc gcggcgagat cgagaaaacc cgtgcgacgc tgcgcgatcg agtgtccgcc
     3481 gccggtggac ggctcgcgtg gagtccgacc ggaacgcttg tgctgatggt catcgctgcc
     3541 gcggcggccg tcgtcggttt gtggccgcgg ctcaaggagt tcgtgtgaga tcgcaagttg
     3601 cgatggcgtt acttgtactt gcgctcgtcg ccgccggatg tggatcctcg acatcgccga
     3661 cgtcggtgac ggtcgcgcca atcgatgcac ggccagcggg tgcgcaggag gtgacgaacg
     3721 cgccggttgc ggggatggcg gactgcgatc gggaagccag cctgcgccca ggcccgcagc
     3781 cttcgccggg tgccatgcca cccggttcga cgatggcggc gatcgccgag cgcgggcggc
     3841 ttgtcgtcgg tgtcgaccag aacacctatc cttttggctt ccggaacccc tcttcggggc
     3901 agctcgaggg cttcgacatc gatctcgccc gcgaggtcgc gcgccgcatc ttcggcgatc
     3961 cagaccgcat cgatctgcgg gtggtcgatg caagccaacg ggaatcggcg ttgcagtcgg
     4021 gtcaggtgga cgtggtggtc cggacctact cgatcacctg cgaccgcaaa cgcagcgtcg
     4081 aattctccac ggtgtactac tacgccaacc agcgcatcct cgccgcgaag ggttccggaa
     4141 tcgattctgc cgcagccctt tcgggcaaac gggtgtgtgc ggtgaggggc acgacctcgc
     4201 tgtcggcgat gttcgcgctc aaccccaggc cgacactgtt cagcgtgacc acctggaccg
     4261 attgcctcgt gatggtgcaa cagggacaga ttgacgcgat cagcaccgac gacgtggttc
     4321 tgtttgggct cgccgcgcag gacccgactg tcgaggtggt tggtcccagc atcgccgtcg
     4381 agccctatgg cgtcgggatc aagaaggaga acatcgatct ggttcgattc gtcaacggtg
     4441 ccctcgacca gatgcgcgcc gatggaacgt gggaacgcct ctatgaggcc cacctgcgaa
     4501 gcctcggacc gtcgcccggt ccgccgccag cgcggtatgg ggactgatgg gaaaagacga
     4561 aatgacatgc gccgatccgg tatgcgccgg cactgtcgtc ggcggctact gcgacctctg
     4621 cggcgccgcc tccgcaacct ctgcgccgca agggccgtcg atggcgactg cctggagcgg
     4681 ttcgaacgct cgatcggtgc aaacggctcg catcaggtcg tcgaagtctg ccagcgcccg
     4741 tggccgactc ggcgccggga ttgtcgcgat accgcgcata ccgacggtcg atccagccgc
     4801 cgcgatcatg accgacccgc aggtgccgga gcctaaccgg ttctgcggca accctgaatg
     4861 caacaagcct gtcgggcgtg gacgggacgg caaccccgga cggccggagg gcttctgcac
     4921 ccagtgcggc acgcgttact cgttcgttcc caagctctct cgcggcgacc tcgtcggcgg
     4981 gcaatacgaa gtgcagggat gcattgcgca cggtggcctc ggctggatct acctagcgat
     5041 cgaccgcaac gtgcacaacc gctgggtggt gctcaagggc ctcctgaact ccggcgatgc
     5101 caacgcgatg gccgtcgccg ctgccgaggt cctggctctg gccgaagtgg aacaccccaa
     5161 catcgttcgg atctacaact tcgtcgagca tgtcgacgcc gccggggtcc cggtcggcta
     5221 catcgtgatg gagtacgtcg gcggcaggtc gctgaagcaa atccgcaaag cccgcaacgc
     5281 tcctcttccc ccggaccagg ccgtcgccta catcgtcgag atcgcatctg cactccacta
     5341 cttgcacacg caaggcctcg cctactgcga cttcaagccg gacaacgtga tgcagaccga
     5401 cgagcagctc aagctgatcg acctcggcgc agtcgtcgcc atggacgacg aggaaagcgc
     5461 gatctacggg acccgcggct accaggcacc cgagatcgcc aagaccggcc ccaccgtcgc
     5521 cagtgacgtt tacgccgtcg ggcgcacgct cgccgttctc gtgatggatg ttccacagga
     5581 gcacggacat ttcgtcgagc agctacccgg gcccgcgacc atgccggtgc tcgccaagca
     5641 cgaatcgttg tatcgcgcga tcctccgcgc caccgacccc gatccggggc ggcgattctc
     5701 gtccatggca gagatggccg accaactgac cggggtgctg cacgagatcg cggccgccga
     5761 cagtgacatt gcgcagccac ggatgtctaa ccacttcagc ccgcagcccg cggtctacgg
     5821 tgcaggccgg gacgcgccgg tggaaccggc tgatgtgatc gcggcg
//
LOCUS       DASWBF010000405         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_771921,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000405 DASWBF010000000
VERSION     DASWBF010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771921"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VF874_15005"
     CDS             <1..791
                     /locus_tag="VF874_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEX9177325.1"
                     /translation="KDVVSPVGPIYPSERQAAPLAVACHEGKSRFHGTGIHPGGVTEV
                     FPLMFSAMSSAVTFVRAEEFSDIRTYGAPDVVRHIMCFGGTREEAEQGSMVKGLGSGF
                     KQSIHMVLQALGLAGRAEIRTVHGVAVATAPIESPIGTIEPGKVAGQRFHWEAVVDGE
                     VVVRVGVTWLMGEESLDPPWSFGDQRERYEIEVKGNPDAFVTIKGWQPETVAAGLVSS
                     PGIVATAAHCVNAVPYVCAAPTGIVTSLELPLVAGRAHPKLLGG"
     gene            707..>1654
                     /locus_tag="VF874_15010"
     CDS             707..>1654
                     /locus_tag="VF874_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEX9177326.1"
                     /translation="MRGTDGNRHVVGAAAGRRPRAPEVARRLTPAWTSLQRLHRAVGL
                     ARIIDPALAVHPQADAESVLHAVELAKIGFEPEEIVLILRLLMDGLTHAAIVMCQTAL
                     QHLLHPGSTELELAQAAEAMARQTDPVLGPMVEDLLRLALRHSFQTDAVNATERAAGA
                     LPNARDVAVAFADLVGFTRLGEAVGPEPLARLASGLGDRAREVAQDPVQFVKTIGDAV
                     MFVSAERRALLHTVLDLVDAVAADALPRLRVGVAYGLAASYAADWYGRAVNVASRITA
                     VAPPRAVYAEESARRTIGDVLGIAWQEIGARRLKGVRDEA"
BASE COUNT          270 a          529 c          576 g          279 t
ORIGIN      
        1 gcaaggacgt cgtcagcccg gtcgggccga tttacccctc cgagcggcag gccgcaccgc
       61 tggcagtcgc gtgtcacgag ggcaagtcgc ggtttcacgg cacaggcatt cacccaggcg
      121 gtgtcaccga agtcttccca ctgatgttct cggcgatgtc gtcggcggtc acattcgttc
      181 gcgctgaaga gttttcggac atcagaacct atggggcccc cgacgtcgtc cggcacatca
      241 tgtgtttcgg cgggacacgg gaagaggccg agcaagggtc gatggtcaag ggcctgggca
      301 gtggtttcaa gcagtcgatc cacatggtgc tgcaggcgct cggtctcgcg ggcagggcgg
      361 agatccgcac cgtacatggc gtcgcggtcg ccaccgcgcc gatcgagtcg ccgatcggca
      421 cgatcgaacc gggcaaggtc gccggccagc ggttccattg ggaagccgtc gtcgacggcg
      481 aggtcgtggt gcgcgtcggg gtcacctggc tgatggggga ggagagcctc gatcccccat
      541 ggagtttcgg tgatcagcgt gaacgctacg agatcgaggt caagggaaat cccgacgcgt
      601 tcgtgaccat caagggctgg cagccggaga ccgtggcggc cgggttggtg agtagcccgg
      661 gaatcgttgc cacagcggca cattgcgtga acgcagtgcc ctatgtatgc gcggcaccga
      721 cgggaatcgt cacgtcgttg gagctgccgc tggtcgccgg ccgcgcgcac ccgaagttgc
      781 tcggcggcta acacctgcat ggacgtcgct gcaacgactt caccgcgccg tcggactcgc
      841 aaggatcatt gatcccgcac tggcggtaca tccgcaagcc gacgcggagt cggtgctgca
      901 tgcggtcgag ttggcgaaga tcggcttcga gcctgaggaa atcgtgctga tcctgcgcct
      961 gctcatggac ggtctcacgc acgccgcgat cgtcatgtgc cagacagccc ttcagcatct
     1021 gctgcacccc ggatccaccg aacttgagct ggcacaggct gccgaggcga tggcgcgcca
     1081 aaccgaccca gtgctcggcc ccatggttga agacctgttg cgcctggcgc tccggcactc
     1141 gtttcaaacc gacgctgtca acgcgaccga gcgggcagcc ggtgcactgc caaatgctcg
     1201 cgatgtcgcg gtggcgttcg ctgatctcgt cggcttcacc cgcctcggcg aggcagtggg
     1261 ccctgagccc ctcgcccgcc tggccagtgg gctgggtgac cgggcacgag aggtcgcgca
     1321 agatcccgtt cagttcgtga agacgatcgg cgacgcagtc atgttcgtca gcgcggagcg
     1381 tcgcgcgctg ctgcacaccg tcctcgacct cgtcgacgcg gtcgcagcgg acgcgctgcc
     1441 ccggctgcgg gtgggcgtcg cgtacggtct ggccgccagc tacgcggccg actggtacgg
     1501 cagagccgtg aacgtggcca gccgcatcac cgcggtcgca ccgccgaggg cggtctatgc
     1561 cgaggagtcg gctcgccgaa cgattggcga tgtgctgggc atcgcctggc aggagatcgg
     1621 tgcccgacgg ctcaagggtg tccgcgacga ggcc
//
LOCUS       DASWBF010000406        14860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_698244,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000406 DASWBF010000000
VERSION     DASWBF010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14860
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698244"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF874_15015"
     CDS             complement(<1..225)
                     /locus_tag="VF874_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEX9177327.1"
                     /translation="MPKSKVRKKNDFTVRPVSRTPVKVKAGPSSVWFTAFFIGLMLIG
                     LAWLLVYQLGASGPTPPSFLQWMADMEPWKN"
     gene            309..1046
                     /locus_tag="VF874_15020"
     CDS             309..1046
                     /locus_tag="VF874_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX9177328.1"
                     /translation="MVTRRTAWRLGVPLVCLLAGVLLATTHGVSGGSEIRRSDSPRLV
                     DLVREAQQSVDRLSAERDALAAQVDAARAGPASNDSQVAALLAKAAPLAVDAGLTPVR
                     GPGLVVTLTDAQRDANGRFPRDASPDDLVVHEQDIQAVLNALWSGGAEAVQMQDQRLI
                     ATSAVRCVGNTLLLNGRTYSPPYTVTAVGDVPKMQESLAAAPLVTLYKQYVVRFGLGY
                     TEEPRAAVDVVGYTAPVAMHYARPAER"
     gene            1104..1778
                     /locus_tag="VF874_15025"
     CDS             1104..1778
                     /locus_tag="VF874_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX9177329.1"
                     /translation="MRVLVVDNYDSFVFNLVQYLGQLGVQAQVWRNDDPNLADPIAVA
                     DDFDGVLLSPGPGTPERAGASIPLVKACATTATPLLGVCLGHQAIGAAFGATVDRAPE
                     LLHGKTSTVYHTNTGVLHGLPDPFVATRYHSLTVLPETMPAELEVIARTESGVIMALQ
                     HVKLPIHGVQFHPESILTQGGHRMLANWLGICGSAPDEALVSRLENEVADTVRAALLP
                     TRRTSA"
     gene            complement(1756..3630)
                     /gene="pknB"
                     /locus_tag="VF874_15030"
     CDS             complement(1756..3630)
                     /gene="pknB"
                     /locus_tag="VF874_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX9177330.1"
                     /translation="MTTPQHLSDRYELGEILGFGGMSEVHLARDLRLHRDVAVKVLRA
                     DLARDPSFYLRFRREAQNAAALNHPAIVAVYDTGEAETTAGPLPYIVMEYVDGITLRD
                     IVHNEGPIPPRKAIEIIADACQALNFSHQHGIIHRDVKPANIMISRTGAVKVMDFGIA
                     RALADSHSVTQTAAVIGTAQYLSPEQARGDSVDARSDVYSLGCVLYEIVTGEPPFVGD
                     SPVSVAYQHVREAPVPPSQRHEGVSADLDAVVLKALAKNPENRYQTAAEMRADLVRVH
                     NGEPPEAPKVLTDADRTSLLTSAANRGPQPIEVAPRQPDYGAGRRTGRGSVGRWLIAV
                     AVLAVLLVAVYWALDAINGPTHSVQIPSVKNQPSADAIATLQNKGFKIRTLQKPDSTV
                     APDHVIDTDPKEGEAVEQGSEVTINVSTGPEQREIPDVANLSYADAVRRLTAAGFGKV
                     KQVPTPSTPEQKDRVIGTNPPANQTAPVTFEISVLVGSGPASVDVPDVTGQTEDQART
                     TLTAAGFTNIISSPADSPRAPGTVLGTNPPAAQSVPKDTVIQLQVSRGNQFVMPDLTG
                     MFWTDAEPQLRALGWTGILVKGADVPGNDQQRNRVVYQSPGPGSGVNRDGNVTLKFGA
                     "
     gene            complement(3816..4901)
                     /locus_tag="VF874_15035"
                     /pseudo
     CDS             complement(3816..4901)
                     /locus_tag="VF874_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
     gene            complement(4898..6376)
                     /gene="pbpA"
                     /locus_tag="VF874_15040"
     CDS             complement(4898..6376)
                     /gene="pbpA"
                     /locus_tag="VF874_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-transpeptidase PbpA"
                     /protein_id="HEX9177331.1"
                     /translation="MNTSLRRVSVTIMVLVVLLLLNATITQVFRADGLRTDPRNQRVL
                     LDEYSRQRGQISAGGQPLAYSVSTPGRFRYLRVYPDPLAAAPVTGFYSLNYSSSGLER
                     AEDSILNGSDERLFARRLADFFTGRDPRGGNVDTTIKPQVQQAAWNAMAKGCSGGPCT
                     GAVVALEPSTGKILAMVSAPSYDPNLLASHDVEAQTQAWQRLRDDPNNPMLNRAISET
                     YAPGSTFKVITTAAALQAGTTEDEQLTASPSIQLPDSTASLENFGGNPCGSGPTASLK
                     EAFARSCNTAFVQLGLKTGADALRTTAAAFGLDTAPKPIPLQVAESTVGPIPDQAALG
                     MSAIGQKDVALTPLQNAMVAAAIANRGSMMQPYLVDSLEGPDLTTISTNQPQELRRAV
                     SQQVAAKLTELMIGAEQVTQQKGAIPGVQIASKTGTAEHGTDPRNTPPHAWYIAFAPA
                     QSPRVAVAVIVENGDNQLSATGGALAAPIGRAVIEAALREGT"
     gene            complement(6373..7788)
                     /locus_tag="VF874_15045"
     CDS             complement(6373..7788)
                     /locus_tag="VF874_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX9177332.1"
                     /translation="MTTQPQPVVAVTPPLPNRRNVELLLLGFAAVITTVALLIVEANQ
                     EQGIRWDLAQYVVAYLALFGAAHVAIRRFAPYADPLLLPVVALLNGLGLVMIHRLDLA
                     EGTLVSGPAQGPSANQQMLWTLVGVSAFSLVVVFLRDHRMLARYGYLCGFAGLVLLVI
                     PALLPSSISEENGAKIWIRLPGFSIQPAEFSKILLLVFFAAVLVAKRGVFTSAGKHFL
                     GMNLPRPRDLAPLLAAWIISVGVMVFEKDLGTSLLLYASFLVMVYIATERLSWVIIGL
                     ALFAAGSVAAYHLFEHVETRVQTWLDPFADPDGSGYQMVQSMFSFATGGVFGTGLGNG
                     QPSTVPAASTDFIIAVVGEELGLVGLAGVLMLYTIVIIRGLRTAIAVRDSFGKLLAAG
                     LASTLAIQLFIVVGGVTNLIPLTGLTTPWMSYGGSSLLANYVLLAILVRISHAARRPI
                     GIRPPQATPIAAASTEVIERV"
     gene            complement(7785..9302)
                     /locus_tag="VF874_15050"
     CDS             complement(7785..9302)
                     /locus_tag="VF874_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX9177333.1"
                     /translation="MTLVLRYAARSDRGLVRANNEDSVYAGARLLALADGMGGHAAGE
                     VASQLVIAALAHLDDDEPGGDLLAKLDQAVREGNAAIAAHVENDPELEGMGTTLTAIL
                     FAGNKLGLVHIGDSRGYMLRDGELAQITKDDTFVQTLVDDGRITAEEAHSHPQRSLIM
                     RALTGHEVEPTLNMREARAGDRYLLCSDGLSDPVSQETILEALQLPDVAESADRLIEL
                     ALRGGGPDNVTVVVADVVDYDYGQTQPILAGAVSGDDDQIAPPNTSAGRASAFNPKRI
                     AAAKRVVAEPEPPAKRGRPWRRILIAVAVVVALLVAGLIAGYQIISSHYYVAAVHDGT
                     VSVMRGYEGSLLGYPLQHQSRVGCLNNQNALSIFDLGQQPDGCRQLKVDDLNEKGRTQ
                     IAGLPGGSLNDATEQMRKLVEQSLLPVCRQTTPTTPPPPAPTLTKVPENTQEPTTTSP
                     TPTTTPSRPTNPTETSEPPLTNTPSPSLTTSRTTPPSVTSTPTPHEPGKDCRKAA"
     gene            complement(9299..9766)
                     /locus_tag="VF874_15055"
     CDS             complement(9299..9766)
                     /locus_tag="VF874_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9177334.1"
                     /translation="MQGLVLQLSRAGFLLLLWLFIFSVLRILRTDIYAPTGAVMVRRG
                     LALRGSLLPNRQRRHTASYLIVTDGALAGTRITLGNQPVLIGRADDSTLVLTDDYAST
                     RHARLSQHGSEWYVEDLGSTNGTYLDRDKVTTAVRVPIGTPVRIGKTVIELRP"
     gene            complement(9868..11274)
                     /locus_tag="VF874_15060"
     CDS             complement(9868..11274)
                     /locus_tag="VF874_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEX9177335.1"
                     /translation="MGFVQRIERKLEVTVGDVAARVFGGAIVPQEVEAALRREASDGV
                     QTLEGGHLLAPNEYVITLSEPDYAEAVADPVLTSDTFAKHLVGYIRDQGWQTYGDLVV
                     RFEQSPKLHTGQFRARAAVNPDAMPAPPQPPHEPDAQPGVAPMTDNPSYRSGQGQGRP
                     DEYYDERYGRPPEDPRGPAGPGPDPRAAYPPPPDQGGYPDQGGYPPPQGEPGYPPRGG
                     YPDQGGYPQQGGYPQQGGYPDQGYTPGYEQPPAPGYGPPPSGYEQQPYRQGPPAGGYG
                     GQPGYGDYGRPPGPPPGPPPGRHEGPGYGGPETQYDYDPGGYQQQGQPYGQPDYGQPD
                     YGRYDAPPGYGAPQAGEYEHGQGSYAPGGYGPNDYGPVSHTTVTLQLDDGSGRTYQLR
                     EGANVIGRGQDAQFRLPDTGVSRRHLEIRWDGQIALLSDLNSTNGTTVNNAPVQEWQL
                     ADGDVIRLGHSEIVVRMH"
     gene            11508..11590
                     /locus_tag="VF874_15065"
     tRNA            11508..11590
                     /locus_tag="VF874_15065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11541..11543,aa:Leu,seq:cag)
     gene            12185..13405
                     /locus_tag="VF874_15070"
     CDS             12185..13405
                     /locus_tag="VF874_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177336.1"
                     /translation="MWDDGYWQVYQQVQRPGGTEWTGAGADAALARVSGDRIQVVGGV
                     DELYAGSLAARNGAWQLNTARQLVLDAVNEAQVAGFHVGEDYSVTSREPVPPTKQTAV
                     QAQAQTFVAKIRGQVLALMCADHVVAAQISTATADLNGLTFDSPRSRATERTPAIQAA
                     GFDRRLVPQKPPKPVPEPPPGSWSPDPLMRAAQKIAYGHASDPITGHMDDFPEMTKDQ
                     LADLTYQKMQRAINDPRGLRLGASNSDGAPVIYDPQDNVLIVRDTRPNAPDGGTVFKP
                     DPVRDPDFVDDKFGWHESIFAPGQLADGPIAAPPSPPLPATNQGNTGVPGGPIGGSIN
                     TAPPRAAPAPAAGGSGGGGSWGDPSPTPRAIPGWGTYVPPDQAAQSDGPIGILGKILQ
                     QFLPPDPNDPNNTA"
     gene            13544..13885
                     /locus_tag="VF874_15075"
     CDS             13544..13885
                     /locus_tag="VF874_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177337.1"
                     /translation="MVLVLNEYAGTAQQAYELMGPVVGQSTRHEWSRLVFPLMGAIDR
                     KEALSDLDWTRALFLAEVGFGSDLVGSGRRFGRGTDEFWIVELRSLQDKISNYHRYTL
                     LRDNAAYPPAT"
     gene            13928..14401
                     /locus_tag="VF874_15080"
     CDS             13928..14401
                     /locus_tag="VF874_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177338.1"
                     /translation="MPGREEFPVTADNDELVDIDLTNTERELMVQVLNEYAGTAQQAF
                     ELMAPVVGRSSEDEWVNYVFPLKQAIKRKEPLSELDWARALFLTEVGFASDLVGSGRR
                     FGRRADEFWIVELRSLQDKISNYHRYTVLRDNAAYPPATRSPSSSSSDTPGLSHF"
     gene            complement(14538..>14860)
                     /locus_tag="VF874_15085"
     CDS             complement(14538..>14860)
                     /locus_tag="VF874_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177339.1"
                     /translation="PSHLTGGHAAGDVAQQHRVIGDLCRIQNGSAGYAVPLGGSDGEL
                     GTLVVLREGVPADRVDPVDDIDDLQVRRGGNCDGDTQSGSAFEAVLVAEEALSTPGMD
                     RMAV"
BASE COUNT         2397 a         4835 c         5057 g         2571 t
ORIGIN      
        1 gttcttccac ggctccatgt cggccatcca ctgaaggaag gacggcggcg tcggccccga
       61 cgcccccagc tggtacacca gcagccacgc cagcccgatc agcatcaggc cgatgaagaa
      121 cgcggtgaac cacacgctcg atgggcccgc cttcaccttg acgggcgtac gactcaccgg
      181 cctgacggtg aagtcgttct tcttacggac cttggacttc ggcatgggct cctctcccgc
      241 tgctagtgca ttgagattat cgcaaccact cgaatcccga ttcgcgcacg atgagagatg
      301 atgcacacgt ggtaacgcgg cgcacggcct ggcgcttggg tgtgccgctg gtgtgcctgt
      361 tggcgggcgt tctcctcgcc accacccacg gagtgtccgg cggcagcgaa atccggcgca
      421 gcgactcccc tcgactggtg gacctcgtcc gtgaagcgca gcagtccgtc gaccgactca
      481 gcgccgagcg cgacgcgttg gccgcccagg tggacgccgc gcgcgccggt ccggccagta
      541 acgactcaca ggtggcggcg ctgctggcca aggccgcccc cctggccgtc gatgcgggcc
      601 tgacgccggt gcgcggaccg ggactagtag tcaccctcac cgacgcacag cgtgacgcca
      661 acggccggtt tccgcgcgac gcgtcgccgg acgatctcgt cgtccacgaa caagacatcc
      721 aggcggtgct caacgcgttg tggagcggcg gcgccgaggc cgtgcagatg caggatcaac
      781 ggctgatcgc cacctcggcg gtccgttgcg tcggtaacac gttgttgctc aacggaagaa
      841 cttatagccc gccctacacc gtcactgccg tcggcgacgt tcccaagatg caggagtcgc
      901 tggctgcagc tccactggta accctgtaca agcagtacgt cgtccggttc gggttgggtt
      961 acacggaaga accgagggcc gcggtggacg tggtcggata caccgccccg gtcgcgatgc
     1021 actacgcccg cccggccgag cgatgagcgc ttgcgcgaag agcagatagc agcgatgagc
     1081 gcttgcgcga gtaacctggc acggtgcgtg ttctggtcgt cgacaactac gacagcttcg
     1141 tctttaacct cgtgcagtac ctcgggcaac tcggggtgca ggcccaagtt tggcgcaacg
     1201 acgacccgaa cctcgccgat ccgatcgcgg tcgccgacga cttcgacggc gtgctgctga
     1261 gtcctggccc aggtacgccg gagcgggcgg gagcgtcgat tccgctcgtc aaggcgtgcg
     1321 cgacgacggc tactcccctg ctcggggtct gcctgggcca ccaggccatc ggcgccgcgt
     1381 tcggcgccac tgtcgaccgc gcgcccgagc tgctgcacgg caagaccagc accgtgtacc
     1441 acacgaatac cggcgtgctg cacggacttc cggatccttt tgtcgccacc agataccact
     1501 cgctgacggt gctgccggag acgatgcctg ccgagctcga ggtgattgcg cgtaccgaga
     1561 gcggggtgat catggcgctg cagcatgtaa agctgccgat ccacggcgtg caattccatc
     1621 ctgagtcgat cctgacccag ggcggccacc ggatgctggc gaactggctg ggcatctgcg
     1681 gttcggcccc ggacgaggcg ctggtttctc gactggagaa cgaggtggcc gacacggtgc
     1741 gcgcggcgct gttacctacg cgccgaactt cagcgtgacg ttgccgtcgc ggttgacgcc
     1801 gctgcccggc cccgggctct ggtagacgac gcggttgcgc tgctggtcgt tgcccggcac
     1861 gtcggcgccc ttgaccaaga tgccggtcca gcccagtgcc cgcagttgcg gctcggcgtc
     1921 cgtccagaac atgccggtca ggtcgggcat cacgaactgg tttcccctcg acacctgcaa
     1981 ctggatcacg gtgtctttcg gaaccgactg ggcggcaggc ggattggtgc ccaagacggt
     2041 accaggggct ctgggactgt cggcaggcga ggagatgatg ttcgtgaagc cggccgcggt
     2101 gagcgtcgtc ctggcctggt cctcggtctg accggtgacg tcgggaacgt cgacgctcgc
     2161 cggtcccgat cccaccagca cggaaatctc gaacgtcacc ggcgccgtct ggttcgccgg
     2221 cggattggtg ccgatcaccc ggtccttctg ttccggagtc gacggtgtgg gaacttgctt
     2281 gaccttgccg aagcccgcag ccgtcaacct gcggacggcg tcggcatagc tcaggttcgc
     2341 cacatcgggg atctcgcgtt gctccggacc ggtcgaaaca ttgatcgtca cctcggaccc
     2401 ttgctcgacg gcctcgccct ccttcgggtc ggtgtcgatg acgtggtcgg gtgcgacggt
     2461 cgagtcgggc ttctgcagcg tgcggatctt gaaacccttg ttctgcaggg tcgcgatcgc
     2521 atcggcggac ggctggtttt tcacgctggg aatctgcacc gagtgcgtcg ggccgttgat
     2581 cgcgtcgagg gcccagtaca ccgcgactag cagcacggcc agcaccgcga cagcgatcag
     2641 ccaccgcccc accgacccgc ggccggtgcg tcgccccgcg ccgtagtcgg gctgccgcgg
     2701 ggccacctcg atcggctgcg gcccacggtt ggcggccgag gtcaaaagcg acgtccgatc
     2761 ggcgtcggtc agcaccttgg gcgcctcggg tggctcgccg ttgtgcaccc gcaccaggtc
     2821 ggcgcgcatc tcggccgccg tctggtagcg gttttccggg ttcttggcca gagccttcag
     2881 cacgacggca tccaagtccg cggagacgcc ctcgtgccgc tgagacggcg ggaccggcgc
     2941 ctcgcggaca tgctggtacg ccaccgacac cggcgagtcc ccgacgaaag gtggctcccc
     3001 cgtgacgatt tcgtagagta cacagcccag cgagtacacg tcggagcggg cgtcgacgga
     3061 gtcgccgcgg gcctgctcgg gcgacagata ctgggcggtg ccgatgacgg ccgcagtctg
     3121 ggtgacgctg tggctgtcgg ccaacgcgcg ggcgatcccg aagtccatca ccttcaccgc
     3181 gccggtgcgg ctgatcatga tgttggccgg cttcacgtcg cggtggatga tgccgtgctg
     3241 gtggctgaag ttcagcgcct ggcaggcgtc ggcaatgatc tcgatcgcct tgcgcggcgg
     3301 aatcggacct tcgttgtgca cgatgtcgcg cagcgtgatc ccgtcgacgt actccatgac
     3361 gatgtagggc agcgggcccg cggtggtctc cgcctctccg gtgtcataca ccgcgacgat
     3421 cgcggggtgg ttcagagccg cggcgttctg cgcctcccgg cggaaccgaa gatagaagct
     3481 cggatcacgg gcgaggtcgg cccgcagcac cttgaccgcc acgtcgcggt gcagccgcaa
     3541 gtcacgcgcc aggtgaacct cggacatccc gccgaagccg aggatctcgc cgagttcgta
     3601 gcgatcggac agatgctggg gggtggtcat gccgcgttct cgtcgtgtgg tggcggcgac
     3661 tcgtgagcac ctgccgccgc gggggagtac ccagtctggg ggttggagcc gggcagcgtc
     3721 caattctggg gggtcgccgg agccggctcg gtctgggtga tggtcgtctc cagcggcggc
     3781 tggtgatgct gctctcgctt gtcgagccac tggttgatca acagcaccgc gatcacgatc
     3841 gccagcgcgc ccagcacccc cgccgcccac agcagcgcgc gctgacccga cgagaacgtg
     3901 cgccgcggcg gcggcggccg atgcgagccg gtcgctggcc gcacccgcgc ggtcatggct
     3961 ccggaccgcg gggtcgtgtc ggcagccgcg cgcggctgtg ctccggacgg gatggccgcc
     4021 ggcgcggccc ggttgatcgt cggcgccccg ttgggtcgcg gaggtcgacg ccccgcccgg
     4081 accgcggcga cggcgtcggc gaactgcccg ccgttgcggt agcgcacgcc cgggttcttg
     4141 gccagggtga tctcgatcag ctcgcgcacg ttcgggggca ggtctgccgg caacggcgcg
     4201 ggcgtctctt tgatgtgctt catcgccaca gtcagcgcgc cgtcgccggt gaatggccgc
     4261 ttacccgaaa ccgcttcgta gccaacgact cccagtgagt aaacgtcact ggcggcggtg
     4321 gcgtcgtgac cgagggcctg ctccggtgcg atgtactgtg cggtgcccat caccatcccg
     4381 gtctgggtca ccggagcggc gtcgacggcc ttggcgatgc cgaagtcggt gatcttcacc
     4441 tggccggtcg gggtgatcaa gatgttcccg ggcttgacgt cgcggtgcac cagccccgcg
     4501 gtgtgggcga cctgcagcgc gcggccggtc tgctcgagca tgtcgagtga gtgccgcagc
     4561 gacagccgac cggtgcgctt gagcaccgag ttcagcggct cgccgttgac cagctccatc
     4621 accaggtacg cggtgcggcc ctcggcgtcc atctcggttt cgccgtagtc gtacacactc
     4681 gcgatgcccg gatggttgag catcgcggtg gtgcgcgcct cggcgcggaa ccgttcgatg
     4741 aactcggggt ccgacgagaa ctccgccttg agcaccttga ccgccacccg gcggcccagg
     4801 cggctgtcga cggcctccca cacctggccc ataccgcccg tcgcgatgag ccgctgcagc
     4861 cggtaccgcc cggacagcgt caccccgacc cgggcgctca tgtgccctcc cgaagcgccg
     4921 cctcgatcac cgctcgcccg atgggcgcgg ccagcgcgcc accggtggcc gacagctggt
     4981 tgtcgccgtt ctccacgatc accgcgacgg cgacccgggg actctgggcc ggcgcgaacg
     5041 cgatgtacca ggcatgcggt ggggtgttgc gcgggtcggt gccatgctcg gccgtcccgg
     5101 tcttggacgc aatctgcacg ccgggaatgg cccctttctg ctgagttacc tgttcggcac
     5161 cgatcatcag ttccgtcagc ttagctgcga cctgttgcga cacggcacgg cgcagttcct
     5221 gcggctggtt ggtgctgatc gtggtcaggt cggggccctc caggctgtcg accaggtagg
     5281 gctgcatcat cgacccccgg ttcgcgatcg cggcggccac catcgcgttc tgcagcggcg
     5341 tcagcgcgac gtccttctgg ccgatggccg acatcccgag ggcggcctgg tcggggatcg
     5401 ggccgacggt ggattctgcg acttgcagcg gaatcggttt tggcgctgtg tccagaccga
     5461 atgccgccgc tgtggtgcgc agcgcgtcgg cgcccgtctt gagtccgagc tgcacgaacg
     5521 cggtgttgca ggaccgcgcg aacgcttcct tgagcgacgc ggtgggcccg ctgccgcacg
     5581 gattgccccc gaagttctcc aaagacgctg tgctgtcggg caattggatc gacggactgg
     5641 cggtcaactg ctcgtcttca gtcgtgccgg cttgcagcgc cgcggcggtg gtgatcacct
     5701 tgaacgtcga cccgggcgcg tacgtctcgg aaatcgcccg gttgagcatc gggttgttcg
     5761 ggtcgtcgcg cagccgctgc cacgcttgtg tctgcgcctc cacgtcgtgc gatgcgagca
     5821 ggttggggtc gtaggacggc gccgacacca tcgcgaggat tttgccggtc gacggctcga
     5881 gggccaccac cgcgcccgtg cacggcccgc cgctgcagcc cttcgccatc gcgttccaag
     5941 ccgcttgctg cacttggggt ttgatcgtgg tgtcgacgtt tccgccgcgc gggtcacgtc
     6001 cggtgaagaa gtccgcgagc cgacgcgcga acagccgttc gtcggagccg ttgagtatcg
     6061 aatcctctgc tcgctccagc ccgctgctgg agtagttcag cgagtagaag ccggtgaccg
     6121 gcgccgccgc caggggatcg ggatacacgc gcaggtaccg gaaccgcccc ggcgtcgaca
     6181 ccgagtacgc cagcggctgg ccgccggccg agatctgtcc gcgctgacgg gagtactcgt
     6241 cgagcagcac ccgctggttg cggggatcgg tgcgcaaccc gtcggcccgg aacacctgcg
     6301 tgatcgtcgc gttcaacagc aacaggacaa ccagcaccat gatcgtcacc gaaacccggc
     6361 gcagtgaggt gttcatacgc gctcgatcac ctcggtgctt gccgccgcga tcggcgtcgc
     6421 ctgcggcggc cggatgccga tcggccggcg cgcggcgtgg ctgatccgca ccagaatcgc
     6481 cagcagcaca tagttggcca gcagcgacga cccgccgtag gacatccacg gcgtcgtcag
     6541 acccgtcagg ggaatcagat tggtgacacc gccgacgacg atgaacagct ggatcgccag
     6601 tgtggaggcc agccctgccg ccagcagctt gccgaagctg tcgcggaccg cgatggcggt
     6661 gcgcagcccg cggatgatca cgatcgtgta cagcatcagc accccggcga gcccgaccag
     6721 gccgagctcc tcgccgacca cggcgatgat gaagtccgtc gacgccgccg gcacggtgct
     6781 cggctggccg ttgcccaggc cggtgccgaa taccccgccg gtggcgaagc tgaacatcga
     6841 ttgcaccatc tggtagccac tgccgtcagg gtcggcgaac gggtcgagcc acgtctgcac
     6901 gcgggtctcg acgtgctcga aaaggtgata tgcggcaacg cttcccgcgg cgaacagcgc
     6961 cagcccgatg atcacccagc tgagtcgttc ggtggcgatg tacaccatca ccaggaacga
     7021 cgcatatagc aaaagcgatg tgcccaaatc cttttcgaag accatcacgc ccaccgagat
     7081 gatccacgcc gcgagcaggg gagcgaggtc gcgtggccgc ggtaggttca tgcccaggaa
     7141 gtgcttaccc gcgctggtga acaccccccg cttggccacc agcaccgcgg cgaaaaacac
     7201 cagcagcaag atcttcgaga actctgcggg ctgaattgag aagcccggca agcgaatcca
     7261 gatcttcgcg ccgttctctt cggagatcga cgacggcaat agggccggaa tcacgaggag
     7321 gaccaacccg gcgaagccgc agaggtagcc gtaccgggcg agcatccggt ggtctcgcag
     7381 gaacacgacc accagtgaga acgccgacac gccgaccagc gtccacagca tctgctggtt
     7441 ggcgctgggc ccctgcgccg gaccggaaac cagcgtgccc tcggccaggt caagccggtg
     7501 gatcatcact agccccagcc cgttgagcag cgcgacgacc ggcagcagca gcggatcggc
     7561 gtacggcgcg aaccgacgga tcgccacgtg tgccgccccg aacagcgcca ggtaggcgac
     7621 gacgtactgc gccaaatccc aacggatgcc ttgttcttgg tttgcttcga cgatcagcag
     7681 ggccaccgtt gtgatgacgg ccgcaaaccc gagtaacagc agttcaacat tgcgccgatt
     7741 gggcagcggc ggcgtcaccg ccaccaccgg ctgcggctgg gtggtcatgc ggccttccgg
     7801 cagtctttcc ccggttcatg aggcgtcgga gtgctggtca cggacggggg agtggtgcgc
     7861 gatgtggtaa gtgacggcga cggcgtgttt gtcaacggcg gctcggaggt ctctgtggga
     7921 ttggtgggtc tcgacggggt ggtggtcggg gtcggcgagg tagtcgtggg ttcttgagtg
     7981 ttctccggga ctttggtcaa ggtaggcgcc ggcggcggcg gcgtcgtcgg ggtggtctgc
     8041 cggcataccg gcaacaggga ctgctctacc aacttgcgca tctgttcggt tgcatcgttg
     8101 aggcttccgc ctggtaagcc ggcgatctgg gtgcggccct tctcgttcaa gtcgtccacc
     8161 ttgagctgtc tgcacccgtc cggttgttga cccaagtcga agatcgacaa cgcgttctgg
     8221 ttgttgaggc agccgacccg agattggtgc tgcagtgggt agccgagcag cgacccttca
     8281 tagccgcgca tgacggacac cgtgccgtcg tggacggcgg cgacgtagta gtggctgctg
     8341 atgatctggt accccgcgat cagaccggcc accaggagag cgacgacaac ggccacggcg
     8401 atgaggatgc gacgccaggg ccgtccgcgc tttgcaggtg gttcaggttc tgccacaacg
     8461 cgtttcgcgg cggcaatgcg cttcgggttg aaggccgatg cacggcccgc cgacgtgttc
     8521 gggggtgcga tctgatcgtc gtcgccagag acggcgcctg ccaggatcgg ctgggtctgt
     8581 ccgtagtcgt agtcgacgac gtcggcgacc accacggtga cgttgtcagg gccgccaccg
     8641 cgcaacgcca attcgatgag gcggtcggcg ctttcggcga cgtcgggcag ctgcagcgct
     8701 tcgaggatgg tctcctggct gaccggatcg gacaggccgt ccgagcacaa caggtagcgg
     8761 tcgccggcgc gcgcctcccg catgttcagc gtcggctcca cctcgtggcc ggtcagtgcg
     8821 cgcatgatga gcgaccgctg cgggtggctg tgtgcctctt cggcggtgat acggccgtcg
     8881 tcgacgaggg tctgcacgaa cgtgtcgtcc ttggtgatct gcgcgagctc gccgtcacgg
     8941 agcatgtagc cgcgcgagtc gccgatgtgc acgaggccaa gcttgttgcc cgcgaacagg
     9001 attgcggtga gcgtggtgcc catgccttcg agctcggggt cgttttcgac gtgggcggcg
     9061 atcgccgcgt tgccttcgcg caccgcctgg tcgagcttgg cgagaaggtc gccgccgggc
     9121 tcgtcgtcgt cgaggtgtgc cagcgccgcg attaccagtt gggaggcgac ctcgccggcg
     9181 gcgtggccgc ccatcccgtc ggcgagcgcg agtagtcgcg cccccgcgta gacagagtct
     9241 tcgttattgg cgcgcaccag tccgcggtca ctgcgcgcgg cataacggag gacaagcgtc
     9301 acgggcgcaa ctcgattacc gtcttgccga ttcgaaccgg cgtgccgatc ggaacgcgta
     9361 ccgccgtcgt caccttgtcc ctatcgagat atgtaccgtt ggtcgatcct aggtcttcga
     9421 cgtaccactc ggagccatgc tgcgacagcc gggcatggcg cgtcgaagcg tagtcgtcgg
     9481 tcagcacgag cgtggagtca tcggcacgcc cgatgagcac gggctggttg cccaacgtga
     9541 ttcgggttcc ggcgagtgca ccgtcggtga ctatgaggta gctggccgtg tgacgacgct
     9601 gccggttagg cagcagtgag ccgcgcagtg ccagcccccg gcgcaccatc actgcgccgg
     9661 tcggggcgta gatgtcggtg cgcagaattc gcagtacgga gaaaatgaac agccacagca
     9721 acagaaggaa gccggcgcgc gacagttgca gcaccaaccc ctgcatctgg cgcccccttc
     9781 gtcctcgtgg ccgacggccc gtcagcaacg tcacgatact tgggcggacc tcggcgcggg
     9841 aggcacgccg acagcgcgtc cgcggcttca gtgcatgcgg acgacgattt cggagtgccc
     9901 gagccggatg acgtcgccgt cggcgagttg ccactcctgc accggtgcat tgttcacggt
     9961 ggtgccgttg gtggagttga ggtcagacag caacgcgatc tggccgtccc accgaatctc
    10021 gaggtgacga cgggagacgc cggtgtcggg cagccggaac tgcgcgtcct ggccacggcc
    10081 gattacgttg gcgccctccc tgagttgata cgtgcggccg ctgccgtcat cgagctgcaa
    10141 cgtgacggtg gtgtggctga cggggccgta gtcgttcggg ccgtagccgc cgggcgcata
    10201 actgccctgt ccgtgctcat actcgccggc ctgaggagcg ccgtatccgg gcggcgcgtc
    10261 gtagcggccg tagtcgggct gaccgtagtc gggttgaccg tagggctgac cctgctgctg
    10321 gtagccgccc gggtcgtagt cgtactgcgt ttcggggccg ccgtagccgg ggccttcgtg
    10381 gcgtcccggc ggcggtccgg ggggcggtcc ggggggccgg ccgtagtcgc cgtaaccggg
    10441 ctggccgccg tagccgccgg cgggcgggcc ttgtcggtag ggctgttgct cgtatccact
    10501 cgggggcggg ccatatcccg gtgccggcgg ctgttcgtat cccggggtgt agccctggtc
    10561 ggggtaaccg ccttgctgcg gatagccgcc ttgctgcgga tagccgcctt ggtcggggta
    10621 gccaccgcgg ggcgggtacc cgggctcacc ctggggtggc gggtagccgc cctggtcggg
    10681 gtagccgccc tggtccggag gcggcggata ggcggcccgt ggatcggggc cgggaccggc
    10741 gggaccgcgc gggtcttccg gcggacggcc gtagcgttcg tcgtagtact cgtcggggcg
    10801 cccctgtccc tggccgctgc ggtagctggg attgtctgtc atcggtgcta ctcctggttg
    10861 tgcgtcgggc tcatgaggtg gttgtggcgg ggcgggcatg gcgtcggggt tgaccgcagc
    10921 gcgggcgcga aactgaccgg tgtgcagctt cggcgactgc tcgaatcgga caaccagatc
    10981 accatacgtt tgccatccct gatcgcggat gtagcccacc agatgcttgg cgaaagtatc
    11041 ggacgtgaga accggatcgg ccaccgcctc ggcatagtcg ggttcactga gcgtgatcac
    11101 gtactcgttc ggcgcgagca gatggccgcc ctcgagggtt tgtacgccgt cggaagcctc
    11161 ccgccgcagc gcggcttcga cttcttgcgg gacgatggcg ccgccgaaca cgcgggcagc
    11221 tacgtcgccg acggtgactt caagtttgcg ttcgatgcgc tgcacaaaac ccatgtcacc
    11281 gtcctttcct atttatcagc ccgcgaacgc aacgccccgc gtgtcctgcc atcacttcgt
    11341 tctgttgcat ggtatcggtc ctgataacca cagcgggaac cagagaactc tgagaatccg
    11401 atcggccctt gtcagagccg gttccgggcc gaattcacca cgttttcaac accatgatcg
    11461 agttggggcc gcgggtcgct accgtgataa cctctagcgg ttatttcggg cgagtggcgg
    11521 aatggcagac gcgctggctt caggtgccag tgtccttcgg gacgtggggg ttcaagtccc
    11581 ccttcgccca ccgagagcgg ttccacgaac taagaggtat taggtcacga actcgaaagg
    11641 gttcctaacc gttgggtctg tggcgcaggc tgcgttgctt cacggcgtcg agcgcacacc
    11701 gcgttgttgg gcctgatgtc gtccgggggt gcagaaatat gcggtaaggg cccagcctga
    11761 cccaccctta gagtcgtctt cgtgggcgat tcaatccggg cggatgtcac cgggctgtcg
    11821 gtactggcgg cgtgctgcga gaaccaggcg gctgccgtca ggggcagctc ggggcgaccg
    11881 ctttggctgg cgacggtttt ccgcgacggc ggcggtgggc gctgcattcg agattgccac
    11941 agcagcgggc catcggatat ccgaccggct cgactcgaca gctaccgcga gcaccggcgc
    12001 ggcggccggt taccgcagca ctgaattaca gagcgccgtg cgcatcgcgg cggtcgcacc
    12061 gtcgtcgagc gcgacgatcg aggtctgatg cccgcggcgt ccgtgccgtc cctgtcacag
    12121 gtccggtcat ggaaataccg actacctcga cggcgccgcg aattcgtgga ccagcacggc
    12181 gcaggtgtgg gacgacggct attggcaggt gtaccaacaa gtgcaacggc ccggcggcac
    12241 ggagtggacg ggtgcgggcg ctgatgcggc tcttgcccgg gtatctggcg atcgcattca
    12301 ggtggttggt ggggtcgacg agttgtacgc cgggtccctc gcggcgcgca acggcgcctg
    12361 gcaactgaac accgcccgcc agttggtcct cgacgcggtg aacgaggcgc aggtcgcagg
    12421 gttccacgtc ggtgaggact attcggtgac gagtcgcgaa ccggtgccgc cgacgaagca
    12481 gaccgccgtg caggctcaag cacagacctt cgtggcaaag atccgcggtc aggtgctggc
    12541 actgatgtgc gctgatcacg tggtggccgc ccagatcagc accgccaccg ccgacttgaa
    12601 cggccttact ttcgattcgc ctagatcgcg ggcgaccgag cgcacccccg caatccaggc
    12661 cgccggtttt gacaggaggc tcgtcccgca aaagccaccg aagccggtgc cggagccgcc
    12721 tccgggcagc tggagccccg atccgttgat gcgggccgcg cagaagatcg cctatgggca
    12781 cgcatctgac cccatcacag gacacatgga cgacttcccc gaaatgacga aagaccaact
    12841 cgccgacctg acctaccaaa agatgcaacg ggccataaac gatccccgcg ggctcagact
    12901 cggtgcaagc aatagcgacg gcgcaccggt catttacgac ccgcaagaca acgtgttgat
    12961 cgtgcgtgac acgcggccga acgccccgga cggcggaacc gtattcaaac cggacccggt
    13021 cagggatccc gacttcgtcg atgacaagtt cggttggcac gaatcgatct tcgcgccggg
    13081 ccaactcgcc gacggcccga tcgccgcgcc gccatcccca ccgttgcccg caactaatca
    13141 aggcaacact ggtgtgccgg gaggccctat cgggggcagc atcaacaccg cgccaccaag
    13201 ggccgccccc gcgcctgcag ctggtggcag cggtggcgga ggcagctggg gagatcccag
    13261 ccctactcct cgagccattc cgggctgggg cacctacgtg ccacccgatc aggccgctca
    13321 aagcgacggc ccgatcggca tcctcggaaa gatcctccaa cagttcctgc cgcccgaccc
    13381 taacgacccc aacaacacgg cttagggatg ctgacggcgt cgaggcggca cggatgtcgg
    13441 caacggtgac aacatcgaag tgacgccggt acgaaaggag tccctgtgac aacggacaac
    13501 gatggcctga tggagatcga cctgaccgac accgagcgcg ctgatggtgc tggttctgaa
    13561 cgagtacgcc gggaccgcgc aacaggcgta tgaattgatg ggtccggtgg tcgggcagtc
    13621 cacccgccat gagtggtcac gccttgtctt ccccttgatg ggagctatcg accgcaagga
    13681 ggcattgtca gatctggact ggacgcgggc gttgtttttg gccgaggtcg gcttcggcag
    13741 cgatttggtc ggctcgggac ggcgattcgg acgcggaacc gacgagttct ggatcgtcga
    13801 attgcgttca cttcaagaca aaatcagcaa ttaccaccgg tacaccctgc tgcgagacaa
    13861 cgccgcgtat ccccccgcga cttgacacga gatctgacgc actccaagag ctggaaaggg
    13921 gcttgcggtg cctggccgag aggaatttcc tgtgactgct gacaacgatg agctcgttga
    13981 catcgacctg accaacaccg aacgcgagct gatggtgcag gttctcaatg agtacgccgg
    14041 gactgcgcag caagccttcg agttgatggc tccggtggtc ggccggtcca gcgaagatga
    14101 gtgggtgaat tatgtctttc ccttgaagca agccatcaag cgtaaagagc cgctgtcgga
    14161 actggactgg gcgcgggcgc tgttcttgac cgaggtcggc ttcgccagcg acctggtcgg
    14221 ctcgggacgg cggttcggac ggcgcgccga cgagttctgg atcgtcgaat tgcgttcact
    14281 tcaagacaaa atcagcaatt accaccggta caccgtgctg cgagacaacg ccgcgtatcc
    14341 ccccgcgacc cgatcacctt cgtctagctc tagcgatacc cccgggctgt ctcatttcta
    14401 aagatacgaa acacccgtat gagcagtagg ccccggttta tccgacgctt agcagtgttc
    14461 cttgcggcag gggttgccgc ggcggcagtg gccgcctgca gcggcgcgcc cgaccgacgc
    14521 gccgaggccg acgaccttca aaccgccata cggtccatgc ccggcgtcga gagcgcctcc
    14581 tcggctacga gaacagcttc gaacgcggag ccactctgag tatctccgtc gcagttccca
    14641 ccgcgacgca cctgcagatc gtcgatatcg tcaaccggat caacgcggtc cgcggggacg
    14701 ccttcgcgaa gtacgaccag agtgccgagt tctccgtcac tcccaccaag tgggaccgcg
    14761 tatcccgcgg agccgttttg aatgcgacac agatcgccga tgacgcgatg ctgctgcgcg
    14821 acatcgccgg ccgcgtgccc gcccgtgaga tgagatgggg
//
LOCUS       DASWBF010000407        16448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_772106,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000407 DASWBF010000000
VERSION     DASWBF010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16448
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772106"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VF874_15090"
     CDS             complement(<1..335)
                     /locus_tag="VF874_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9177340.1"
                     /translation="MRDIQELIAAARGGSTRAAGRLLSLVESARRDEVLTALGTSTAR
                     VVGITGPPGAGKSTTVAALVGAYREQGLRVAVLAVDPSSPYSGGALLGDRIRMAAHIN
                     DPGVLIRSVA"
     gene            complement(347..1486)
                     /locus_tag="VF874_15095"
     CDS             complement(347..1486)
                     /locus_tag="VF874_15095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9177341.1"
                     /translation="MPEAVIVSALRAPIGTAMKGTLRDTDAYQLANHVVSAIAADLDG
                     NLVDDVVLGEGLYGGGVVARYAALTAGLTSAPGLALNRHCAAGQAAVQGAAASIKAGM
                     DELVIAGGVNSASTSPRFSKIVDGEPVAWFPPTHPDRPDAPNMDMSITVGWNAAVKAG
                     VSREEMDAWALGSHRKAVQAIDEGRFKNEIVPIETPHGLFEVDEHPRRDTTMEKLAAL
                     KPLHPEIEGFSITAGNACGANDGAAVLAVASDRLGLPALATVRAWASVGVDPAITGLA
                     PVDAIPKALARAGISIDDVDLFEINEAFASMCVATTKLLDIDPDRVNPNGSGCSLGHP
                     VAATGARMLVTLVHELRRRGGGIGVAAMCAGGGMGSATVIEVPAP"
     gene            1567..2331
                     /locus_tag="VF874_15100"
     CDS             1567..2331
                     /locus_tag="VF874_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9177342.1"
                     /translation="MQISGSSAVVVGGTGGFGEATVRRLHGAGAKVVVADVADDKGKA
                     LESELGIRYVRTDATSTDDLNTALAEAESLGPLRISVDCHGGPASGGRLVGRDGSPLD
                     LDGFKTTIEFYLTAVFNVMRLSAAAIARQEPLDGGVRGVIVNTASIAGYEGQIGQVPY
                     SAAKGGVIGMTLVAARDLSPLGIRVVTIAPGIFLTPAYPMPPEQLEAHWAPQIPFPKR
                     MGQPSEYAAMVASIVENDYLNGEVIRLDGALRFPPK"
     gene            2339..3091
                     /locus_tag="VF874_15105"
     CDS             2339..3091
                     /locus_tag="VF874_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9177343.1"
                     /translation="MTGTLAGKVAFVAGASRGIGATVAAALAREGAAVAVAARSESEG
                     RVPGTIHAVAERIVAAGGRARAVRCDVTDESSVDAAVAETVAEFGGIDILIANAGVLW
                     LGPIESTPLKRWRLCLDVNLTGTFLVTKAVIPHVRARGGGSLVAITTTGVTMEGANAY
                     WVSKAGVERLYLGLAADLKPDNIAVNCLSPSRVVLTEGWFAGVGGQEIPPEMVEPPEA
                     MADAAVLLARQDASGITGTIQRSEELVPPRDS"
     gene            complement(3088..4017)
                     /locus_tag="VF874_15110"
     CDS             complement(3088..4017)
                     /locus_tag="VF874_15110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX9177344.1"
                     /translation="MRIGLTGGASSIDKMIAQAEQAEADGFSSLWYASTVAGDPLVAM
                     ALAGRATSTIELGTAVLQTYPCHPMLQANRTAAAANAMGRPGFTLGIGPSHGSLITDV
                     YGLSYDHPGRSTEEYLRIITALLRGADVDFEGHDWTTRSAGRMTPLAHPVPVLLSALS
                     PHLLRVAGQYADGAVLWMATEKVIESHVAPRINAAADDAGRPEPRIVAGLPVAVHDDI
                     GEARAAAAATATVYAGMRNYQRILEVGGLSDAAEAAIVGGEASVRAQLQGLLEAGATD
                     VWAAVFPVGKEKADRIASLRSTTDLLRELALEV"
     gene            complement(4031..5401)
                     /locus_tag="VF874_15115"
     CDS             complement(4031..5401)
                     /locus_tag="VF874_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9177345.1"
                     /translation="MTITSEHVAGDADRKIPRYHFDRHAADYRDRFLTITHKMQARCP
                     IAWSETYGGHWVAASADAVFELTRCPHVSNDHDIHNERRGYKGISIPTMIESPPTRGG
                     MLEMDDPEHRIYRTALNPYLSPAAVKRWVPFVDEVVRACLDDKVEGGQIDFVDDLANV
                     VPAVLTLAMLGIPPANWTFYNEPAHASVYTPPHSPEAARVEELYMAMGLDMYKNLTEI
                     RDKPRPGIVDALTRIRVDGQAPDDMELLGILNLLIGGGFDTTTALTAHAFEWLSQNPE
                     GRDMLSRERDTLLDTATEEFLRYFTPAPGDGRTISEDCDVAGAHFKEGERLWLSWAMA
                     NRDPSVFSDPDRILIGRTANRHFSFGLGVHRCIGSNVARTVFKSMLTAVLDRIPDYRC
                     DPERTVHYDTIGVIQGMRHLPATFTPASRRGAGLDETLEKLQRVCDEQGLARPITELK
                     QSAHIP"
     gene            complement(5385..5531)
                     /locus_tag="VF874_15120"
                     /pseudo
     CDS             complement(5385..5531)
                     /locus_tag="VF874_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928261.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(5543..6286)
                     /locus_tag="VF874_15125"
     CDS             complement(5543..6286)
                     /locus_tag="VF874_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9177346.1"
                     /translation="MSKVAVVTGGGSGLGLSICQHLARQDRAVAVLDMDGEAAERVVG
                     GLRAEGAQAIAVEVDVSDQPAVGASFDKVRAELGTIEILVTSAGISGFTPFEDITLDE
                     WNRYVAVNLTGTFLCIQAAIPDMVASGWGRIVTISSAAGQVGALRQGHYSATKGGVIA
                     MTKTVALEYASRGITVNTVPPFTADTPMLRAAQQAKKLPSAEVISRMIPAGRVGTGDE
                     IAAVCVFLCSDAASYITGQVIGVNGGAVL"
     gene            6395..7171
                     /locus_tag="VF874_15130"
     CDS             6395..7171
                     /locus_tag="VF874_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9177347.1"
                     /translation="MGKWDPAFTEQLYKKAGPFIRKYFRAEVRGLESVPAAGGALIVS
                     NHSGGMMTLDVPIFGAAFYDKFGYDRPLYTLAHNQVLVGPFKEWLGRAGIIHASRENA
                     ATALRSGAIVLVFPGGDHDSYRPTLNQNVIDFNGRKGYVKTAIETGVPIVPVVSIGGQ
                     ETQFFLTRGNWLAKRVGLKRLRVEILPISIGLPFGLTTFLVPNFPLPAKIVTQVLKPV
                     DVLAEFGKDPEVDEVDAHVRSVMQGALDWMARQRRFPLLG"
     gene            complement(7168..7938)
                     /locus_tag="VF874_15135"
     CDS             complement(7168..7938)
                     /locus_tag="VF874_15135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177348.1"
                     /translation="MNGRFSLDGRVAIITGGGTGIGRGAALVLAEHGADVVLAGRRPD
                     PLESTAKEVEALGRRAVAAPADVTSAAACRGLVDMTLREFGHVDILVNSAGGAETKSI
                     LKWPDDDFEHVMALNFGAVWHLSRAAAKPMLEQGKGAIVNISSGASLLAMPQAAPYGA
                     AKAAVNNLTGSMAAAWTRKGIRVNAIAVGAVRAATLLDDAARYGLDPEAIAMTNASGR
                     LGEPDEIGYGVLFFASDASSFCSGQTLYMHGGPGPAGV"
     gene            8025..9263
                     /locus_tag="VF874_15140"
     CDS             8025..9263
                     /locus_tag="VF874_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9177349.1"
                     /translation="MNDLYYDPWDVEIDLDPYPVYRRLRDEMPLYYNERHDFWGISRY
                     ADVDAALRDPARLSSAKGDILEVVKADPVMPPGVFINEDPPLHTIHRALVSRAFMPKR
                     IKAVEDKVRAFCVACLDPLVGSERFDFVQDLGAELPMRTIGMLVGIPDADQPAVRAQA
                     HRVLRNKPGEPLPVKKDHYFDGDMFTEYLAWRQKNPSDDLITELLSVEFEDETGTTRR
                     LRTDELLIFLAVIAGAGVETTGRLFGWMGKVLGEHPDQRQQLVEDRALIPNAIEELLR
                     YEPPGPHVARYVATEDVEFHGQTVPAGSALLIMLASANRDERHFENADRFDINRRPGG
                     HLTFGRGAHFCLGAPLARLEGRIALEEVLNRFPKWDIDIDNAHRSRTSTVRGWDSMPA
                     IVSCHSGGSGMPLVSTPAST"
     gene            complement(9212..9805)
                     /locus_tag="VF874_15145"
     CDS             complement(9212..9805)
                     /locus_tag="VF874_15145"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9177350.1"
                     /translation="MSDQPRLNPLPPELWDDDSVAALRGAFSDELIEMFRTPGPTPNV
                     LATMLHHPALAGPFNVYGNVLLQQPAIGHRARELMLLRVAWRTGALYEWVHHVRLAPS
                     YGVAAEDIDAITRGVDADSWTPLERDLVAAADQLLDHYRIDDDTWARLAEELDERQLV
                     EIPFVVGTYTCLAMAFNSWGLQVEAGVDTSGIPLPPE"
     gene            9873..10685
                     /locus_tag="VF874_15150"
     CDS             9873..10685
                     /locus_tag="VF874_15150"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177351.1"
                     /translation="MTLTPESLGTAPGRGRLTGRRIVVVGAGTRPSEDPDAPIGNGRG
                     IAVLCAREGANVACVDIDRDAAQFTANLCEQEGAKASTIVADVRDADECERLVNEAHD
                     GLGGLDGLVANVGFGAGAGLAGTSPELWDDIFALNVRSHFLVARAALPILEDGSSIVF
                     IGSAASLRAGTRFPAYDASKAALFGVCRHVALEGAPRGIRANHVIPGPVDTPLGRYGS
                     ANVERRSQIRLPLGRQATAWDIAYPTVFMLSGESSYITGESLVVAGGLGQMG"
     gene            complement(10695..11333)
                     /locus_tag="VF874_15155"
     CDS             complement(10695..11333)
                     /locus_tag="VF874_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9177352.1"
                     /translation="MAASPRRVGAETSKTRDVLIDSVERLMSEEGYASVTYRAVAAKA
                     GVTPSLVQYYFPTLDDIFLATIRRRTDRNVTRLTEALEEHPDDPLRVLWQYSWAEATG
                     ALITEFMALGNHRKSIRAEIAEVTEKVRRVQLDALERRFAKGVHLPEGELSPGALLLL
                     ITGIPKFLNLEEGIGVKTAHGEVVEAFERYLGAAEPRRNIRKRKPPSRSPRR"
     gene            11421..11999
                     /locus_tag="VF874_15160"
     CDS             11421..11999
                     /locus_tag="VF874_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9177353.1"
                     /translation="MTVDTDLDTLQRNVRYLLDRTAILDCIVRHARGCDRHDVDLICA
                     AYHEDGVDEHGKAINPGPEYGNWANATHAATSQVHTHNITTHTCEVDGDTAHAESYVI
                     VVLLGRDGRSAQFISGRYLDRLERRNGEWRIAVRRSTVEVMFLADASVLQSSFFKDTG
                     YLVGTRDRHDLSYERPLSIDTPAPARWAISAR"
     gene            complement(11983..12747)
                     /locus_tag="VF874_15165"
     CDS             complement(11983..12747)
                     /locus_tag="VF874_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9177354.1"
                     /translation="MYDMPNEVEVEADGALRIIRLNRPDALNAVNDDLHVALAHLWPR
                     LNEDRDARAAVITGNGRTFSAGGDFAYLDELGRDPDLRAKTILHGREIVLGMVRTRIP
                     VVAAVNGPAVGLGCSLVALSDIVYMAEGAYLADPHVQVGLVAADGGPLVWPLQISVLQ
                     AKEYALTGIRISAQRAVEIGLANHVVADPLSEAIACAKKIMELPRQAVESTKRMLNMQ
                     VERAVLNTLDYANAAEEQSFQTEDFRQIVARLNAPK"
     gene            complement(12749..13837)
                     /locus_tag="VF874_15170"
     CDS             complement(12749..13837)
                     /locus_tag="VF874_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177355.1"
                     /translation="MDFRDSPEEAAFRERLRTWLSGQAGAFATSGDDYWARQGEWHQA
                     LYSAGFFGLSWPKKFGGQELPPVYDVIVDEELARAGAPPRPSLGYLVVGLGEHGSEEL
                     QQRFLPGMINGTERWCQGFSEPGAGSDLASLRTKATRDGDNYVIEGYKIWTSYSDVAD
                     WCLLLARTDPDAPRHKGISAFIISMHQPGITQRPLQMINGVTTEFGQVEFDGAIVPAD
                     QMVGAPGQGWALAMTVVSHEREPSTLGYSARYGKLVRQMAKRVGGDIPDELVWAAVES
                     EMLRLHVRRRLSEQLDGLSHGPDGSLDKLLMTWTEQSVGHAALSVGGTQDPELLSAYL
                     YSRAQSVMGGTSQIQKNIIASRILGLGV"
     gene            complement(13824..14708)
                     /locus_tag="VF874_15175"
     CDS             complement(13824..14708)
                     /locus_tag="VF874_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177356.1"
                     /translation="MDVRLTPEQRQLRDAAAKLADDLGPGSVQDLSDESRIARLEKAV
                     DATGWRSLRSDGASGVEVAIVAEEFGRGLVDVPFLGPVLADDLLRHLGDSQPATLPVD
                     ARGYARAVTLADTTVSAADIGQTVPTADLTRAMAEVSGVQAIGELTREDCQRFQALAV
                     TATTADILGTARGAHTLAREYAKIREQYGKPIGSYQAVAHMLAEGLALIEGSISVLRH
                     AAWAVDELEPTGAIRAANIAKVYCARAARTVCETAIQVHGGIGNTWECLAHVYLRRAL
                     TSAELFPVSLKEIDLGLS"
     gene            complement(14686..16059)
                     /locus_tag="VF874_15180"
     CDS             complement(14686..16059)
                     /locus_tag="VF874_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9177357.1"
                     /translation="MLTRTAALAFGERHYSHAEIEALTDGLAATLRGRGVRSGQRVAL
                     MSSNRPEFVVAVRAIWRLGAAVVLLSPAWKQSEVQHALALTGPSHAVGDHPVLAELMP
                     MLHLDEPITEGRASFGEPSPESDAVLVFSSGTTGMPKAVRHTHQSFGAAIGHWRSALQ
                     MTSSDRMQIVTPASHILGLLNIMTALDTGAWLRLHPRFDIDLMLHHIEADRITIEMAV
                     APIALALAAHPRLDSVDLSSLRYIMWCATPVTPSVAETVTRRTGVGWLTAYGTSELPV
                     IACNPLDDVRLDTVGRPAPGVRVRVGELGEIQAQSDSLMAGYLPDEATPDAFCDGWYR
                     TGDVGFLDDDGYLRITDRSKEMIKVRGFQVAPAEIERVLHGHPAVQDCAVFGIPDAVY
                     GEAIVAAVAAHAPVGADELVGLVGDRLASYKRPADVVFVPEIPRLPSGKVLRRVLKES
                     YGRSADT"
     gene            16131..>16448
                     /locus_tag="VF874_15185"
     CDS             16131..>16448
                     /locus_tag="VF874_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9177358.1"
                     /translation="MAITHVFHRATVARIVKETPDARTFVLTPDEPFSYRAGQYCTFR
                     VTVDGEDLYRSYSMSSAPETDTELATTVKRVPSGKVSNWMLDNLVEGDEVVLTRPAGT
                     FCLD"
BASE COUNT         2826 a         5469 c         5285 g         2868 t
ORIGIN      
        1 gcgaccgacc gaatcagcac gccggggtcg ttgatgtgcg ctgccatccg gatgcggtct
       61 ccgagaagcg caccaccgct gtacggggac gacgggtcga ccgccagcac cgcgacgcgc
      121 aggccttgct cgcggtacgc cccgacgagt gccgcgaccg tcgtcgactt acccgcgccg
      181 ggcggtcccg tgatcccgac cacgcgggcg gtggacgtac ccagggccgt cagcacctcg
      241 tcgcgacgcg cgctttcgac gaggctgagc aaccggccgg ccgcccgggt ggacccgcct
      301 cgcgccgctg cgatgagctc ctggatgtcc cgcatacgag aagaacttac ggtgcgggga
      361 cctcgatgac cgtcgccgaa cccattccgc cgccggcgca catcgccgcg accccgattc
      421 cgccgcctcg ccggcgcagt tcgtgcacca gcgtgacgag catgcgcgcg cccgttgccg
      481 cgaccgggtg gccgagcgag cagccgcttc cgttggggtt cacgcggtcc gggtcgatgt
      541 cgagcagctt ggtggtggcc acacacatcg aggcgaacgc ctcgttgatc tcgaagagat
      601 cgacatcgtc aatcgaaatt ccggctctgg caagcgcttt cgggatcgcg tcgaccggcg
      661 ccagccccgt gatggcggga tcgacaccga ccgatgccca ggcgcgcacg gtggcaagcg
      721 cgggcaggcc cagccggtcg ctggccaccg ccaacaccgc ggcgccgtcg ttggcgccgc
      781 acgcgttgcc cgcggtgatc gagaacccct cgatttccgg gtgcagcggc ttaagcgcgg
      841 caagcttctc catcgtggtg tcgcggcgcg ggtgctcgtc gacttcgaac agcccatgag
      901 gggtctcgat cgggacgatc tcgttcttga accggccctc gtcgatggcc tgcaccgcct
      961 tgcggtgcga ccccagcgcc caggcgtcca tctcctcgcg gctgacgcct gccttgaccg
     1021 ccgcgttcca gccgacggtg atcgacatgt ccatgttggg agcgtcgggc cggtcgggat
     1081 gggtcggcgg gaaccaggcg accggctcgc cgtcgacgat cttgctgaac cgaggcgacg
     1141 tcgacgccga gttcacgccg ccggcgatga cgagctcgtc catccccgct ttgatactgg
     1201 ccgcggcgcc ctgcacggcg gcctgtccag cggcgcagtg cctgttcagc gctagccctg
     1261 gcgccgacgt cagccctgct gtcagcgcgg cgtagcgagc caccaccccg ccaccgtaca
     1321 gcccctcgcc gaggacgacg tcgtccacca gattcccgtc gagatcggcg gcgatggcgc
     1381 tgaccacgtg attcgcgagc tggtaggcgt cggtgtcgcg cagcgttccc ttcatcgcgg
     1441 tcccgatggg agcgcgcaat gcggacacga tgacggcttc aggcataggt ggtctccaga
     1501 ttttcaagca gtatgagaat gctattctct cagtctgaga gtgcgagttg caagaagggg
     1561 aatcatatgc agatcagcgg gagctccgcc gtcgtcgtgg gcggcactgg aggatttggc
     1621 gaggccaccg ttcgccggct gcacggcgcg ggcgcgaagg tcgtcgtcgc cgacgtggcc
     1681 gacgacaaag gcaaagcgct ggaaagtgag ctcggcatcc gctacgtgcg caccgacgcc
     1741 accagcactg acgacctcaa caccgctctc gcggaggcgg aatcccttgg ccctctgcgt
     1801 atttcggtcg actgccacgg cggccccgcc agcgggggac gtctcgtggg cagggacggc
     1861 tcgccgctcg acctcgacgg cttcaagacg accatcgagt tctacctgac cgcggtgttc
     1921 aacgtgatgc ggctgtccgc ggccgcaatt gcgcgacagg agccgctcga cggcggtgtg
     1981 cgcggtgtga tcgtcaacac tgcatcaatc gccggctacg agggccagat cggccaggtt
     2041 ccatactccg cggcgaaggg cggcgtcatc ggcatgacgc tcgtcgcggc ccgcgacctc
     2101 tcccctcttg gcattcgcgt ggtcaccatt gccccgggga tcttcctgac gcccgcgtac
     2161 ccgatgccgc ccgagcagct cgaggcgcac tgggcaccgc agatcccgtt cccgaagcgg
     2221 atgggccagc cgtccgagta cgcggccatg gtcgcgagca tcgtcgagaa cgactacctc
     2281 aacggcgaag tcatccgcct cgacggggcg ctgcggttcc ccccgaaatg atcgagcagt
     2341 gacgggcacg ctcgcgggaa aggtcgcctt tgtcgcgggc gccagccggg gcatcggcgc
     2401 cacggtggcc gcagccctcg cgcgtgaggg cgcggccgtg gcggtcgccg cgcgctccga
     2461 gtcggagggc cgggtgccgg ggaccatcca tgcggtggcc gagcggatcg tcgcggcggg
     2521 cggccgggcg cgcgccgtgc ggtgcgacgt caccgacgag tcgtccgttg acgccgcggt
     2581 ggccgagacc gttgcggagt tcggcggcat tgacatcctg atcgccaacg cgggcgtgct
     2641 gtggctcgga ccgatcgagt cgacgccgtt gaagcggtgg cgactgtgcc tggacgtcaa
     2701 cctgaccggg acattcctgg tgaccaaggc ggtcattcct catgttcgcg cgcggggagg
     2761 cggctcgctg gtggcgatca cgacgaccgg ggtgacgatg gaaggtgcca acgcctactg
     2821 ggtctccaag gccggtgtcg agcggcttta cctcgggctg gccgccgacc tgaaacccga
     2881 caacatcgcg gtgaactgcc tcagcccgtc gcgggtggtg ctcaccgaag gatggttcgc
     2941 cggggttggc gggcaagaga ttccaccgga gatggtcgag ccgccggaag cgatggccga
     3001 cgccgcggtg ctgctcgccc gccaggacgc cagcggcatc acgggcacga tccagcgctc
     3061 ggaagagctt gtgccgccgc gcgacagtta aacttcgagc gcaagttcac gaagtaggtc
     3121 cgtcgtgctg cgcaacgagg cgattcggtc cgctttttcc ttgccgacgg ggaagaccgc
     3181 ggcccacaca tccgtagctc ccgcctcgag cagcccctgc aactgtgcgc gcaccgatgc
     3241 ttcaccgccg acgatcgcgg cctccgccgc atcactcagg ccgccgacct ccaagatccg
     3301 ttggtagttc cgcatcccgg cgtacaccgt tgcggtagcc gcagccgcgg cacgtgcctc
     3361 gccaatgtcg tcgtgcacag caacaggcaa tccagcgaca atccgcggtt ccggacgacc
     3421 ggcatcatcg gccgctgcgt taatccgcgg tgcgacatga ctttcgatca ccttttcagt
     3481 tgccatccac aacactgcgc cgtcggcgta ctgtcccgcg acccgcaaca ggtgcggaga
     3541 cagcgcggac agcagcaccg gaaccggatg ggccaatgga gtcattcggc cggcactgcg
     3601 cgtggtccag tcgtgtcctt cgaagtcgac gtccgcgccg cgaagcagcg cggtgatgat
     3661 gcgcaggtac tcctcggtgc tacggcccgg atggtcgtag gacaagccat agacgtcggt
     3721 gatgagtgag ccgtgcgatg gcccgatgcc gagcgtaaat ccagggcgtc ccatggcgtt
     3781 tgcggccgct gctgtcctgt ttgcttgcag cattgggtga cacgggtacg tctgcaacac
     3841 tgcagtgccg agctcgatgg tcgacgtggc ccgcccggcc agtgccatcg cgacgagggg
     3901 gtcaccggcg acggtgctgg cataccacag cgagctgaaa ccgtcggcct cagcctgctc
     3961 ggcttgagcg atcattttgt cgatcgacga cgcaccgccg gtcagcccga tccgcattca
     4021 ttgcctccga tcaaggtatg tgcgccgatt gtttcagctc tgtgatcggt ctggcgagtc
     4081 cttgttcgtc gcagacgcgt tggagtttct caagggtttc gtcgagaccg gcgccccgcc
     4141 gcgatgcggg tgtgaaggtc gcgggaagat gccgcatacc ctggatcaca ccgatggtgt
     4201 cgtagtgcac ggtgcgttcg ggatcgcatc ggtagtcggg aatccggtcc agcacagcag
     4261 tgagcatcga cttgaacacc gtgcgcgcaa cattggaacc aatgcagcgg tgaacaccca
     4321 acccgaaact gaaatgcctg tttgctgtgc gcccgatgag gattcgatcc gggtcgctaa
     4381 ataccgaggg gtcccggttg gccatggccc aagacagcca cagccgctct ccctccttga
     4441 agtgcgcgcc ggcgacgtca cagtcttcgg agatcgtgcg tccgtcaccg ggggccggcg
     4501 tgaagtagcg caggaactct tcggtggcag tgtccagcag ggtgtcgcgc tcacggctga
     4561 gcatgtcccg cccttccggg ttctgggaca gccactcgaa cgcgtgcgca gtcaatgcag
     4621 tcgtggtatc gaagccgccg ccgatgagca gattgaggat gcccagcagt tccatgtcgt
     4681 ctggcgcttg tccatcgact cggatccgcg ttaacgcgtc gacgatgccc ggccggggct
     4741 tgtcgcgaat ctcggtcagg ttcttgtaca tatcgaggcc catcgccatg tacaactctt
     4801 ctacccgggc cgcctcaggc gagtgcggcg gcgtgtagac gctggcatgt gcgggctcgt
     4861 tgtaaaaagt ccagtttgca ggcgggatgc ccagcatcgc cagcgtcaga acagccggca
     4921 cgacattggc gaggtcatcc acgaaatcga tctggcctcc ttcgactttg tcgtcgaggc
     4981 acgcgcgtac cacctcgtcg acgaacggca cccatcgttt gacggccgct ggagacagat
     5041 agggattgag ggcagttcgg tagatgcggt gctcgggatc gtccatttcc agcatgccgc
     5101 cccgcgtcgg cggtgactcg atcatcgtcg gaatcgagat gcctttataa ccacggcgtt
     5161 cgttgtggat gtcgtggtcg tttgagacgt gcggacagcg ggtcagctcg aagaccgcat
     5221 ccgcgctcgc agctacccaa tggccgccgt aggtttcgct ccacgcaatc gggcaccgcg
     5281 cctgcatctt atgcgtgatc gtcaggaacc ggtcgcgata atcagcggca tgccggtcga
     5341 agtggtagcg aggtatctta cggtcggcgt cgcccgcgac gtgttcagat gtgatagtca
     5401 taccacttca ggtagccgcc agcgtcgacg cgcaactgca tgcccgtcac gaagcgcgac
     5461 tcatctgagg cgaggaacag cacggcgtcg ctcaggtctt cgggttcgac gtacggggtc
     5521 ggcatggctt gcattccttt cttcacaaga ccgctccgcc gttcacgccg atcacttgcc
     5581 cggtgatata gctcgcggca tccgaacaca agaacacgca gactgcggca atttcgtcgc
     5641 cggtgccgac ccttccggct ggaatcattc gtgagatcac ctcggcgctg ggcagtttct
     5701 tcgcctgctg cgcggcgcgg agcatcgggg tgtctgcggt gaacggcgga acggtgttga
     5761 ccgtgattcc ccgcgacgca tactcgagag ccaccgtctt ggtcatcgcg atgacgccgc
     5821 ccttagtggc cgagtagtgc ccctgacgca acgcgcctac ctgtccggcc gccgaagaga
     5881 tggtgacgat ccggccccac cccgaggcca ccatgtccgg aatcgctgct tgtatacata
     5941 ggaacgtgcc ggtgagattg accgccacgt agcggttcca ctcatccaag gtgatgtctt
     6001 caaagggcgt aaacccggaa atgccagcac ttgtcaccaa gatctcgata gtccccaact
     6061 cggcgcgaac cttgtcgaac gacgcaccga ccgccggctg gtctgacacg tcgacctcga
     6121 ccgctatcgc ctgagcaccc tcggcgcgca ggccgccgac cacccgttcg gccgcctcac
     6181 catccatgtc gagcacggcc accgcgcggt cctggcgggc caggtgttgg cagatcgaca
     6241 gccccagacc ggatccgcct ccggtgacga cggcgacctt actcaatgcg cccagctctt
     6301 cccatcatgc tatacgaccg tatagcctag acgacagtat agcaagcaag gatgcgcagg
     6361 agctgtgagc accgacgaga tcgacaatcc cgagatgggt aagtgggacc ccgccttcac
     6421 cgagcagttg tacaagaagg ccggaccctt catcaggaag tacttccggg cggaggtacg
     6481 cggcctggag tcggttcccg ccgcgggcgg cgctttgatc gtgtcgaacc actccggcgg
     6541 catgatgacc cttgatgtgc cgatctttgg tgccgctttc tatgacaagt tcggctacga
     6601 ccgtccgctg tacactctcg cccacaacca ggtcctcgtc gggcctttca aggagtggct
     6661 tggccgagcc ggcatcatcc acgcgagccg cgagaatgcc gcaactgcgc tgcgctcagg
     6721 tgcaatcgtg ctggtgttcc ccggcggtga ccacgactca taccgaccga cgttgaacca
     6781 gaacgtgatt gacttcaacg gccgcaaggg atacgtcaag accgccatcg agaccggtgt
     6841 gcccatagtg cccgtcgtct cgatcggagg tcaggaaacc cagttcttct tgactcgggg
     6901 caattggctc gcgaaacgag taggtctcaa gcggctgcgc gtcgagattc tgccgatcag
     6961 tattggtctt ccgttcggtc tgaccacgtt cttggtgccg aactttccgc tgccagccaa
     7021 gatcgtgacg caggtgctca aacccgtaga cgtcctggct gagttcggaa aggaccccga
     7081 ggtcgacgag gtggacgccc acgtccgttc ggtgatgcaa ggcgcccttg attggatggc
     7141 tcgtcagcgg cgctttccgc tcctgggcta gacgcccgcc ggccctggac cgccatgcat
     7201 gtacagcgtc tggcccgaac agaaactcga tgcatcagaa gcgaaaaaca ggacgccgta
     7261 accgatctcg tcaggctcac cgaggcgtcc tgacgcattg gtcatagcaa tcgcttcggg
     7321 gtccagcccg taacgtgccg cgtcgtcgag cagcgtcgcc gcacggaccg cgccaacagc
     7381 gatggcgttc acgcggatgc ccttccgcgt ccacgcggcc gccatcgacc cagtcaggtt
     7441 gttcaccgcc gccttggcgg caccgtaggg cgcggcctgc ggcatggcca gcaggctggc
     7501 gcccgaggag atgttgacga tcgctccctt gccctgttcg agcatcggct tggccgccgc
     7561 ccgggagaga tgccagaccg cgccgaagtt gagcgccatc acgtgttcga agtcgtcgtc
     7621 gggccacttc aagatcgact tcgtctcggc accaccggcg ctgttgacca ggatgtcgac
     7681 gtggccgaac tcgcgaagcg tcatgtcaac cagtcctcga cacgccgctg cgctcgtgac
     7741 atcggccggg gcagccacgg cgcgtcgtcc gagcgcctcg acctccttgg ccgtcgactc
     7801 gagaggatca ggacgccggc ccgccagcac gacgtcggca ccatgctcgg cgaggaccag
     7861 cgcggcccct cgtccaatgc ccgtgccgcc accggtgatg atggcgaccc ggccatcgag
     7921 ggaaaaccga ccattcacgt gactgctcct ccgtcgatcg cgttgcctat acgaccgtac
     7981 agcatgtgct atatgctcgt agaaactacc gctgaggagg agtggtgaac gacctctact
     8041 acgacccatg ggacgtcgag atcgatctcg acccgtaccc ggtgtaccga cgactccgcg
     8101 acgagatgcc gctctactac aacgaacgtc acgacttctg gggcatcagt cggtacgccg
     8161 atgtcgatgc ggcgctgaga gatccggcgc ggctcagttc ggccaaaggc gacatcctcg
     8221 aagtcgtcaa agccgatccg gtcatgccgc cgggtgtgtt catcaacgag gacccgcccc
     8281 tgcacaccat ccaccgggca ctggtgtcgc gggcgttcat gccgaagcgg atcaaggccg
     8341 tcgaagacaa ggtccgggcg ttctgcgtcg cgtgcctcga cccgctcgtc ggcagcgaac
     8401 ggttcgactt cgtgcaagac ctcggcgccg aattgccgat gcggaccatc ggcatgctcg
     8461 tcggcattcc ggacgccgac cagccggccg tgcgggccca ggcgcatcgc gtcttgcgca
     8521 acaagccggg tgagccgctg ccggtgaaga aggaccacta tttcgacggc gacatgttca
     8581 ccgagtatct cgcctggcgc cagaagaatc cgtccgacga tctgatcacc gaactgctca
     8641 gtgtcgagtt cgaggacgag acgggcacga cgcgccggct gaggacggac gagcttctga
     8701 tcttcctggc cgtgatcgca ggggcgggcg tggagacgac cggccggctg ttcggatgga
     8761 tgggcaaagt gctgggcgaa cacccggacc agcgccagca gctcgttgag gaccgcgccc
     8821 tcatcccgaa tgcaatcgaa gagttgctgc gctatgagcc ccctggcccg cacgtcgcgc
     8881 ggtatgtcgc cactgaagac gtcgaattcc acgggcagac ggtgcccgcg ggaagcgcgc
     8941 ttctgatcat gcttgcctcc gcgaaccgag acgagcgcca cttcgagaat gcggatcgct
     9001 tcgacatcaa ccgcaggcca ggcggacact tgacgtttgg acggggagca cacttctgcc
     9061 tcggtgcgcc gctggcccgc ctcgaggggc gcatcgcgct ggaggaagtg ctgaaccggt
     9121 tccccaagtg ggacatcgac atcgacaacg cccaccggtc acgcacctca acggtgcgcg
     9181 gctgggactc gatgcccgcg atcgtgagct gtcattccgg tggaagcggg atgccgctgg
     9241 tgtccacgcc ggcttccacc tgaagtcccc aactgttgaa cgccatcgca aggcacgtgt
     9301 aagtgccgac cacgaaaggg atttcgacga gctggcgctc gtcgagctcc tcggccagcc
     9361 gagcccacgt gtcgtcgtcg atgcgatagt ggtcgagcaa ctgatcggct gcggcgacga
     9421 ggtcccgctc gagcggcgtc catgagtcgg cgtcgactcc gcgtgtgatc gcgtcgatgt
     9481 cctctgcagc aaccccgtag ctcggcgcga gccgcacatg gtgcacccac tcgtacagcg
     9541 cccctgttcg ccatgcgacg cgcagcagca tcagctcgcg cgcccgatgc ccgatcgccg
     9601 gctgctgcag caacacgttg ccgtagacgt tgaacgggcc ggcgagcgcc ggatggtgca
     9661 gcatcgtcgc gagcacgttc ggcgtcggac cgggcgtccg gaacatttcg atgagctcgt
     9721 cggaaaatgc gccgcgcagc gcggcaacgg agtcgtcgtc ccagagttcg gggggcaagg
     9781 ggttcagccg cggctgatcg ctcatagttg cggactgtac atcgcgtcag tggcgctgta
     9841 cagccgtaca gcattcgtgt tacgttgcac gaatgaccct cactcctgag tcactcggca
     9901 ccgcgcccgg ccgcggccgg ctgaccggcc gtcgtatcgt cgtcgtcggc gccggcacgc
     9961 ggccatccga ggatcccgat gcaccgatcg gcaatggccg cggcatcgca gtgctgtgcg
    10021 cacgggaggg cgccaacgtc gcgtgcgtcg acatcgacag ggacgccgcg caattcaccg
    10081 cgaacctgtg cgaacaggaa ggcgccaagg cgtcgaccat tgttgcggac gttcgcgacg
    10141 cagatgaatg cgagaggctg gtcaacgaag cgcacgacgg gctcggcgga ctcgacggcc
    10201 tcgtcgcgaa tgtcggcttc ggcgcgggcg cgggcctggc gggcacgtcg cccgaattgt
    10261 gggacgacat cttcgcgttg aacgtgcggt cgcacttcct cgtcgcccgc gcagcgctgc
    10321 cgattctcga ggacggcagc tcgatcgtgt tcattggctc ggccgcaagc ctgcgggccg
    10381 gcacgcgctt tccggcgtac gacgcatcga aagccgctct gttcggcgta tgccgccacg
    10441 tggcgctcga gggtgcgcca cggggcattc gggcgaacca cgtcataccg gggccggtcg
    10501 acacgccgct cggccgttac ggaagtgcca acgtcgagag acgctcgcaa atccgactgc
    10561 cgctgggcag gcaggccacc gcttgggaca ttgcctatcc gacggtgttc atgttgagtg
    10621 gcgaatccag ctacatcacc ggggaatccc tcgttgtcgc aggcggactc gggcagatgg
    10681 gctgagtcgg tggcttagcg gcgaggactc cgcgagggtg gcttgcgttt gcgaatgttg
    10741 cggcgcggct ctgcggctcc gagatagcgc tcgaacgctt ccaccacctc gccatgggcg
    10801 gttttgacgc cgattccctc ttcgaggttg aggaatttcg gaatgcccgt tatcagcagc
    10861 aatagcgcgc cgggcgagag ctcaccttcg gggagatgga cccccttcgc gaatcgccgt
    10921 tccagagcat cgagttggac ccgtcggacc ttctcggtaa cctccgcgat ttcggctcgg
    10981 atggacttcc ggtgattgcc gagcgccatg aattcggtga tcagcgcacc agtggcctcc
    11041 gcccagctgt actgccacag cacccgcaat ggatcgtcgg gatgctcctc caaggcttct
    11101 gtcaaacgcg tgacatttcg atcagtgcga cgtcggatcg tggccaggaa gatgtcgtcg
    11161 agtgtcggaa agtaatactg aacaaggctg ggcgtcacgc cggccttggc cgcgacggca
    11221 cggtaagtga cgctcgcata gccctcctcg ctcatcagcc gctctacgga gtcgataagg
    11281 acatcccgcg tcttggacgt ctcggcgccc acgcgccggg gcgatgctgc catgctcacc
    11341 tcacttattc ggtctgcgtg cattttggca ttcgatgtat gctgtacgat cgtatagtct
    11401 cacattaccg ggagaacacg atgacggtcg acacagattt agacactttg cagcgaaatg
    11461 tccgctactt gttggaccgt acggccatcc tcgactgcat cgtccggcat gcgcgaggat
    11521 gcgatcgcca cgacgtggac ctcatttgcg ctgcctacca cgaggacgga gtggacgaac
    11581 atggcaaggc gatcaacccc ggcccggaat acgggaactg ggccaacgca actcacgccg
    11641 cgacatctca agtccacacg cacaacatca ccacgcacac ctgtgaggtc gacggcgaca
    11701 cggcccacgc cgagagctac gtgatcgtcg tccttctcgg acgcgacggg cgcagtgcgc
    11761 agttcatcag cggccgctac cttgaccgtc tcgagcggcg caatggcgag tggcgcatcg
    11821 cggttcgccg gtcgaccgtc gaggtgatgt tcttggccga cgcgtcggtg ctgcaatcgt
    11881 cgttcttcaa agacaccggc tacctggtcg gcacgcggga tcgccacgac ttgtcctacg
    11941 aacgcccgct gtcgatcgac acgcccgcac ccgcgcgttg ggctatttcg gcgcgttgag
    12001 gcgcgcgacg atctgccgga agtcctcggt ctggaaggac tgctcctctg cggcgttggc
    12061 gtagtccagc gtgttcagca cggcgcgctc gacctgcata ttgagcatcc gcttggtgct
    12121 ctcgacggct tgccgcggca gttccatgat cttcttcgcg caggcgatcg cctccgaaag
    12181 cgggtcggcg acaacgtgat tggccagtcc gatctcgacg gcgcgctgcg ccgagatgcg
    12241 gatgcccgtc aatgcgtact ccttggcctg cagcacgctg atctgcagcg gccagacaag
    12301 cggcccgccg tcggctgcga ccagaccgac ctgcacgtgc gggtccgcga ggtaggcgcc
    12361 ttcggccatg tacacgatgt cggagagcgc caccaggctg cagcccagtc cgaccgcggg
    12421 cccgttgacg gctgccacca ccggaatcct ggtgcgcacc atgccgagaa cgatctcacg
    12481 gccgtgcagg atcgtcttgg ctcgcaggtc gggatcgcgg cccaactcgt cgagatacgc
    12541 gaagtcgccg ccggcggaaa acgtcctacc gttcccggtg ataaccgccg ccctggcgtc
    12601 gcggtcctcg ttcaaccgcg gccacaggtg cgccagcgcg acgtgcaagt cgtcgttgac
    12661 cgcgttcaac gcgtcgggcc gattcagcct gatgattctc agtgcgccgt cggcctcgac
    12721 ctcgacttcg tttggcatgt cgtacacgtc agactcctaa tccgaggatg cgggaagcga
    12781 tgatgttctt ctggatctgc gaggtgccgc ccatgacgct ttgggcccgg ctgtagaggt
    12841 acgcagagag caactccggg tcctgcgttc caccgacgga cagcgcggca tggccgacag
    12901 actgctcggt ccacgtcatc agcagcttgt ccagcgagcc gtccggcccg tgcgacaagc
    12961 cgtcgagttg ttcggagagc ctccgccgca cgtgcagccg caacatctcg gattcgacgg
    13021 ccgcccagac caattcgtca gggatgtcgc caccgacacg tttggccatc tgacgcacta
    13081 gctttccgta ccgggcggag taccccagcg tcgagggctc acgctcgtgg ctaaccacag
    13141 tcatcgccag cgcccagccc tgccccggcg cgccgaccat ctggtcggcc ggaacgatcg
    13201 caccgtcgaa ctccacctga ccgaattccg tcgtaacgcc attgatcatc tgcaggggcc
    13261 gctgtgtgat gccgggctgg tgcatcgaga tgatgaacgc cgagatgccc ttgtgccgtg
    13321 gtgcgtccgg gtcggtgcgc gccaacagca ggcaccagtc ggcgacgtcg gagtagctgg
    13381 tccagatctt gtagccctcg atcacgtagt tgtcgccgtc gcgggtcgcc ttggtccgca
    13441 gcgacgccag gtcggagccg gcgccgggtt ccgaaaagcc ctggcaccag cgctcggtcc
    13501 cgttgatcat gccgggcagg aagcgctgct gaagttcctc gctgccgtgt tccccgaggc
    13561 cgacgacgag ataccccagg ctcggccgcg gcggggcgcc cgcgcgggcc aactcttcgt
    13621 cgacgattac gtcgtagacc ggcggcaatt cctgtccgcc aaactttttc ggccacgaca
    13681 gcccgaagaa ccccgccgag tacaacgcct ggtgccactc gccctgccgt gcccaatagt
    13741 cgtcaccgga ggtggcgaac gccccggcct gcccgctcaa ccacgtccgg aggcgctcgc
    13801 ggaacgcggc ctcctccggc gaatcacgaa agtccaagat cgatctcctt caggctgacc
    13861 gggaacagtt cggcggacgt caacgcgcgc cgcaagtaca cgtgagccag gcattcccaa
    13921 gtgtttccga ttccgccgtg cacctgaatg gcggtctcgc acaccgtgcg ggcggcgcgc
    13981 gcgcagtaga ccttcgcgat gttcgctgcg cggatcgccc cggtcggctc gagttcgtcg
    14041 acggcccacg ccgcgtgccg aagcacactg atcgaaccct cgatcaaggc cagcccttcg
    14101 gcgagcatgt gagccaccgc ttgatacgag ccgatcggct tgccgtactg ctcgcgaatc
    14161 ttggcgtact cacgcgccag tgtgtgggcc cctcgcgcag tcccgaggat gtcggcggtc
    14221 gtcgccgtca ccgcaagcgc ttgaaagcgc tggcagtcct cgcgggtgag ttctccgatc
    14281 gcttggactc cggacacctc cgccatcgcc ctcgtgagat ccgctgtcgg cactgtctgc
    14341 ccgatgtcgg cagccgaaac ggtcgtgtcc gccaacgtca ctgcgcgtgc atacccgcgg
    14401 gcatccaccg gcaatgttgc cggctgggag tcgccgaggt gccgtagcag atcgtcggcg
    14461 agcaccggcc cgaggaacgg gacgtcgacc aggccgcggc cgaattcctc ggcgacaatg
    14521 gcgacttcga cgcccgacgc gccgtccgag cgcagcgacc gccagcccgt cgcgtcgacc
    14581 gccttctcca gtcgcgcgat ccggctctcg tcagacagat cctgcacgga gcccggccca
    14641 aggtcgtcgg ccagtttggc tgcggcatca cgtaattggc gctgctcagg tgtcagccga
    14701 acgtccatag ctctccttca gaactctgcg cagcaccttg cccgagggca ggcggggtat
    14761 ttcgggtacg aacaccacgt cggcggggcg cttgtacgac gccaggcggt cacccaccag
    14821 gccgacgagt tcatcggcgc cgacgggtgc gtgggcggcg accgcggcca cgatcgcttc
    14881 gccgtagacg gcgtcgggga ttccgaacac cgcacagtct tggacggcgg gatgcccgtg
    14941 cagcaccctc tcgatctccg ccggtgcgac ctgaaatccc cgcaccttga tcatttcctt
    15001 cgagcggtcg gtgatccgca agtagccgtc gtcgtcgagg aagcccacgt cgccggtgcg
    15061 gtaccacccg tcacagaacg cgtcgggagt ggcctcgtcg ggcaggtagc cggccatcag
    15121 cgagtccgac tgcgcctgga tctcaccaag ttcgcctacc cgcacgcgca cgccgggagc
    15181 tggacgtccg accgtgtcga gccgcacgtc gtcgagcggg ttgcacgcga tcaccggcag
    15241 ttcacttgtg ccgtaggcgg tcagccatcc cacgccggtt cgacgggtga ccgtctcggc
    15301 gacgctggga gtcacgggcg tggcgcacca catgatgtag cgcagcgacg acaggtcgac
    15361 gctgtccagg cgcggatggg cagcgagtgc caatgcgatt ggtgcgacgg ccatttcgat
    15421 cgtgatgcgg tcggcctcga tgtggtgcag catcagatcg atgtcgaacc ggggatgcag
    15481 ccgaagccag gcgccggtgt ccagcgcggt catgatgttg agcaggccga gaatgtgcga
    15541 ggccggcgtg acgatctgca tccggtccga cgacgtcatc tgcagtgcgc tgcgccagtg
    15601 cccgatcgcg gcgccgaaag actgatgggt gtggcggacg gctttgggca tgccggtggt
    15661 cccggaactg aacaccaaca ccgcgtcgga ttccggactc ggctctccaa acgaagcccg
    15721 gccctcagtg atcggctcgt cgagatgcag catcggcatc agctccgcca agacgggatg
    15781 gtctccgact gcgtggctgg gaccggtgag cgcgagcgcg tgctgcacct cactctgctt
    15841 ccacgccggg ctgagcagaa ccaccgcggc gcccaaccgc cagatcgcac gcacggccac
    15901 gacgaactcg ggccggttcg acgacatcag tgctacgcgc tggccgcttc gcactccccg
    15961 cccccgaagg gtggcggcca acccgtcggt caacgcctcg atttcggcgt ggctgtagtg
    16021 cctctccccg aacgcaagcg cagcagtgcg agtcaacatc gagaggatcc taccgtgatt
    16081 gagaacctga ttctcttatt gatagaatct taataccaga gggaggcggc atggcgatca
    16141 cccacgtgtt ccaccgggcc accgtggctc ggatcgtcaa ggaaacacct gatgcccgca
    16201 ccttcgtgct gacccctgac gagccgtttt cgtatcgggc cggccagtac tgcacgttcc
    16261 gcgtgaccgt ggacggcgaa gacctctatc gctcctactc gatgtcgagc gcccccgaga
    16321 ccgacaccga actggccacc accgtcaaac gcgtaccctc cggcaaggtg tccaactgga
    16381 tgctcgacaa cctggtcgaa ggcgacgagg tggtcttgac cagacccgcc ggaacgttct
    16441 gcctggac
//
LOCUS       DASWBF010000408         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1024991,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000408 DASWBF010000000
VERSION     DASWBF010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024991"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2261)
                     /locus_tag="VF874_15190"
     CDS             complement(<1..2261)
                     /locus_tag="VF874_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9177359.1"
                     /translation="MSSIPDPFARFSPATREWFTGTFAAPTPAQAQAWSAIADGDNTL
                     VIAPTGSGKTLAAFLWAIDRLAHEPGDGNRVLYVSPLKALAVDVERNLRTPLTGITRT
                     AQRLGLPDPRISVGVRTGDTPPRQRRELITRPPDILITTPESLFLMLTSAARDTLAGV
                     QTVIVDEVHAVAGTKRGSHLALSLERLDMMLQRPAQRIGLSATVRPPEEVARFLSGRA
                     PTTIVAPPAAKTFDLSVVVPVPDMANLENNTIWPDVEQRTADLIEAHQSTIVFANSRR
                     LAERLTARLNEIHAERSGAELTSPPNPQVPGGPPAHIMASGQTYGAAPLLARAHHGSV
                     SKEQRAAVEDDLKSGRLKAVVATSSLELGIDMGAVDLVIQVEAPPSVASGLQRIGRAG
                     HQVGEISRGVVFPKHRTDLISCAVTVQRMLAGQIENLAVPANPLDVLAQHTIAACALE
                     PLDADVWFDLVRRSAPFATLPRSAFEATLDLLSGKYPSTEFAELRPRLVYDRDAGMLT
                     ARPGAQRLAVTSGGAIPDRGLFTVYLAAGADSEKPSRVGELDEEMVYESRPGDVISLG
                     ATSWRITEITHDRVLVIPAFGEPARLPFWRGDGVGRPAELGAALGAFTGELAGLTPDA
                     FQKHCAGMGFDEYATENLRRLLDDQRQATNAVPTDSTLVVERFRDELGDWRVILHSPY
                     GLRVHGPLALAVGRRLRERYGIDEKPTASDDGIVVRLPDTEDAPPGADLFVFEPDEIE
                     PTVTAEVGGSALFA"
BASE COUNT          365 a          810 c          867 g          383 t
ORIGIN      
        1 gcgaacagcg ccgagccgcc gacctccgcg gtgacggtcg gctcgatctc gtccggctcg
       61 aagacgaaca ggtcggcacc tgggggtgcg tcctcggtgt cgggaagccg gacgacgatg
      121 ccgtcgtcgg acgcggtggg cttctcgtcg atgccgtatc gctcacgcag ccgccggccg
      181 accgccagcg ccagcggacc gtgcacacgc agtccatacg gcgaatgcag aatcacccgc
      241 cagtcgccga gctcgtcgcg gaaccgctcc accacgagcg tggaatccgt ggggacggcg
      301 ttggtggctt gccgctggtc gtcgagcagg cggcgcaggt tctcggtggc gtactcatcg
      361 aaacccattc ccgcacagtg cttttggaac gcatccggtg tcaggccggc gagttccccg
      421 gtgaacgcgc ctagcgcggc ccccagctcg gcgggccgtc cgacgccgtc cccgcgccag
      481 aacggcaacc tggccggctc gccgaacgcg ggaatcacca gcacgcggtc gtgggtgatc
      541 tcggtgatgc gccaactggt cgcccccagc gagatgacgt cgccgggccg cgactcatac
      601 accatctcct cgtcgagttc gcccacccgc gagggctttt cggagtcggc accggccgcc
      661 aggtacacgg tgaagaggcc gcggtcgggg atcgcgccgc cggaggtgac ggccagccgc
      721 tgcgctccgg gccgggcggt cagcatgccg gcgtcgcggt cgtagaccag tcgcggccgc
      781 agctcggcga attccgtcga cgggtacttg ccggacaaca gatccagcgt cgcctcgaac
      841 gcgctgcgcg gcaacgtcgc aaacggcgca ctgcgccgca ccaagtcgaa ccagacgtcg
      901 gcgtccagcg gctccagcgc gcacgccgcg atggtgtgct gcgccagcac gtcgagcgga
      961 ttggcgggca ccgccaggtt ctcgatctgt ccggccagca tccgctggac ggtgaccgcg
     1021 cagctgatca agtcggtgcg gtgcttcggg aacacgacac cgcgcgaaat ctcgccgacc
     1081 tgatgtcccg cgcggccgat ccgctgcaac ccgctggcca ccgacggcgg cgcctccacc
     1141 tggatcacga ggtcgactgc gcccatgtcg attcctagct cgaggctgga tgtggccacc
     1201 accgctttga gccgcccgct cttgaggtcg tcctcgacgg cggcgcgctg ctccttgctc
     1261 accgagccgt gatgcgcgcg ggccagcagg ggcgcggcgc cgtacgtctg cccgctggcc
     1321 atgatgtgcg cgggcggtcc gcccggaacc tgcggattgg gtggcgacgt cagttcggca
     1381 cctgaacgtt cggcgtgaat ctcgttgagc cgcgcggtca gtcgctcggc cagccgccgg
     1441 gagttcgcga acacgatcgt ggactgatgc gcctctatca gatcggcggt gcgctgctcg
     1501 acgtccggcc agatcgtgtt gttctccaag ttcgccatgt cgggcaccgg caccaccacc
     1561 gacaggtcga atgtcttggc tgccggcggc gcgacgatcg tggtgggcgc acgcccagac
     1621 agaaaccgcg ccacctcctc cggaggacgc acggtggccg atagcccgat gcgctgcgcg
     1681 ggccgttgaa gcatcatgtc gaggcgttcc agcgacagcg cgaggtggga gccgcgcttg
     1741 gtgcctgcga ccgcgtgcac ctcgtcgacg atcacggtct ggacaccggc gagcgtgtcg
     1801 cgggcggccg acgtgagcat caggaacagc gactcggggg tggtgatcag gatgtcgggt
     1861 ggccgggtga tcagttcccg ccgttgtcgc ggcggcgtgt cgccggtgcg gacgccgacg
     1921 ctgatgcgcg gatcgggcaa gccgagccgc tgtgccgtac gggtgatgcc ggtgagcggc
     1981 gtgcgcaggt tgcgttcgac gtcgacggcc agcgccttga gcggggaaac gtagagaacc
     2041 cggttgccgt cgccgggctc gtgagccagc cggtcgatcg cccacaggaa cgccgcgagc
     2101 gtcttgccgg accccgtcgg cgcgatgacc agcgtgttgt cgccgtcggc aatggccgac
     2161 catgcctgcg cctgcgccgg cgtgggcgcg gcgaacgtgc cggtgaacca ctcacgggtg
     2221 gctgggctga agcgggcgaa cgggtcaggg atggagctca cgtgtccatc gtgccaatgg
     2281 ggtacgacaa gaagcgtgct gaacaccgtc accgggtgtg cgacagattt gtaggtacac
     2341 cgaagttgat cttgaattga accgctggcc tgctggtcat gcggtggcgt agctggcgat
     2401 ctggttgtgc ggctgtgtcc agatg
//
LOCUS       DASWBF010000409         9549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_772353,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000409 DASWBF010000000
VERSION     DASWBF010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9549
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772353"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..576)
                     /locus_tag="VF874_15195"
     CDS             complement(337..576)
                     /locus_tag="VF874_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177360.1"
                     /translation="MDDMDRWEDQLARFDAMVSRVPGRGIDVVVHAPDHMDLWKAGRK
                     LAEAVSSVVRSEPVGVEIITEGSYSVDQSSQVCRS"
     gene            complement(688..1167)
                     /locus_tag="VF874_15200"
     CDS             complement(688..1167)
                     /locus_tag="VF874_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177361.1"
                     /translation="MLSRVLTGLAGAVGTVLVMATSPVAGADEAPVLPNINVYPPISP
                     AEFSVMDGTWYAFATPDGLTCVLDKGRNSYGCSGPIPAAPEGANLVSGVGGEEPGFAN
                     TAAPLFAVTGPVKPLPPQTRISYRNVSCAIDAGGATVCVNSATQHGFVLSPAGSFTA"
     gene            complement(1177..1842)
                     /locus_tag="VF874_15205"
     CDS             complement(1177..1842)
                     /locus_tag="VF874_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9177362.1"
                     /translation="MTDQDRAAVRREIDNALVTALERRHDVADAVVEAEDREAAVEAI
                     ATLLGTSRLGAEAVIGMSFDKFTKESRRRIADELDDLNNQLTFTLGERPASTGEGVTL
                     RLFAGADDRDIFSARTADMHVAGDGSGGPAGSLDDEIRSGLGRIDDEDAVWLVAVSGT
                     EKVGMVFGELAGGEVNVRVWIHPEHRKKGYGTAALRKSRSEMASYFPAVPMVVRAPGL
                     HPS"
     gene            complement(1853..2199)
                     /locus_tag="VF874_15210"
                     /pseudo
     CDS             complement(1853..2199)
                     /locus_tag="VF874_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770503.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            complement(2290..3153)
                     /gene="htpX"
                     /locus_tag="VF874_15215"
     CDS             complement(2290..3153)
                     /gene="htpX"
                     /locus_tag="VF874_15215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX9177363.1"
                     /translation="MVGHRWGNGIKTVLLLGLMFGLIVFVGSLFGRNILILSVLFAVG
                     MIVWVYFNSDKLALRAMHAQPVTELQEPTMHRIVRELATVAHQPMPRLYISDTANPNA
                     FATGRNPRNAAVCCTTGILAILNERELRTVLGHELSHVYNRDILISCVAGALAAVISG
                     LANLAMIMSWTGGNRDANPFALLLISLLAPIAATVVRLAVSRSREYQADQSGAELTGD
                     PLALASALRKISAGVQQAPLPPEPKLASESHLMIASPFRSGERMVGLFSTHPPIAERI
                     ARLEAMARGSY"
     gene            complement(3218..4225)
                     /gene="grcC1"
                     /locus_tag="VF874_15220"
     CDS             complement(3218..4225)
                     /gene="grcC1"
                     /locus_tag="VF874_15220"
                     /EC_number="2.5.1.10"
                     /EC_number="2.5.1.29"
                     /EC_number="2.5.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003904430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0052924 - all-trans-nonaprenyl-diphosphate
                     synthase (geranylgeranyl-diphosphate specific) activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0033384 - geranyl diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nonaprenyl/(2E,6E)-farnesyl/geranylgeranyl
                     diphosphat synthase"
                     /protein_id="HEX9177364.1"
                     /translation="MKTPATVVAGVELGDPAFAASVRDGVARIETLISEELGKGDEII
                     RDAVLHLFDAGGKRFRPLFTVLSAQLGPNPDAWQVAVAGSVIELVHLATLYHDDVMDE
                     AQVRRGAPSANARWSNNIAILAGDYLFATASRLVSKLGPDAVRVIAETFAELVTGQMR
                     ETRGAAQHVDSVDHYLRVVHEKTGSLIAASGQFGGTFSGAAEADVQRLRRLGGIVGTA
                     FQISDDIIDIASDSDESGKIPGTDLREGVHTLPVLYALRESGPDAERLRELLAGPMDD
                     DAHVAEALALLRASPGMAEAKDTVARYAAQARDELAGLPDGAGRQALATLVDYTISRH
                     G"
     gene            4241..5467
                     /locus_tag="VF874_15225"
     CDS             4241..5467
                     /locus_tag="VF874_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEX9177365.1"
                     /translation="MSMDASADVVVVGAGPAGSAAAAWAAWAGRDVLVIDAASFPRDK
                     PCGDGLTPRAVAELQRLGLGDWLDAHIKHRGLRLTGFGGEVEIDWPGPSFPAYGSAVP
                     RTELDERVRQVAVDAECRMLLGSKVVEAPRDPRGRVTSLRLSSGSSVACREVIVADGA
                     RSPLGRALGREWHQQTVYGVAVRGYLSTPRASDPWLSTNLELRTRHGHLMPGYGWIFP
                     LGNGEVNIGVGALATQKRPADVALRKLIGHYADLRRHEWDFDGPPRAVSSALLPMGGA
                     VSGVAGPNWMLIGDAAAGVNPLNGEGIDYGLEMGRFAAEMLGSGDLSQAWPALLQEHY
                     AQAFSIARRLAWLLTVPWLLPTVGPFAMRSVAFMQTVVRVMGNLVVDEDVDRVARLWR
                     RLGAGSRFVDRRPPFS"
     gene            5512..5799
                     /locus_tag="VF874_15230"
     CDS             5512..5799
                     /locus_tag="VF874_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9177366.1"
                     /translation="MCHLGAVEQIGIRELRQHASRWVAKAKAGAAILITDRGRPVARL
                     VPITAAEHARETLIAKGLLQPAPRPRPKFGVEQLIDGPPLTEILDDMRSDR"
     gene            5796..6194
                     /locus_tag="VF874_15235"
     CDS             5796..6194
                     /locus_tag="VF874_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9177367.1"
                     /translation="MIYIDTSALVKLVVAEQESSHLIDWLNSRQDQPLVTSVVGHIEL
                     VRVAGRVGEVETAAAQHLAATVDTLTLSDSIANYAATLSLATLRTLDAIHLATAHEYR
                     QDLSTLCAYDHRLIEAARSHRLPVTSPGAG"
     gene            complement(6286..6969)
                     /locus_tag="VF874_15240"
     CDS             complement(6286..6969)
                     /locus_tag="VF874_15240"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HEX9177368.1"
                     /translation="MSRATLDKDPHEVASMFDAVARRYDITNTVLSLGQDRFWRRATR
                     KALDVDAGDRVLDLAAGTAVSTSELARSGAWCVAADFSVGMLLAGKARAVPKVAGDAT
                     RLPFSDQTFDAVTISFGLRNVVDREAALREMARVTRPGGRLVVCEFSTPANPLFGTVY
                     KEYLMQALPRVARAVASNPEAYVYLAESIRAWPDQAELARLIQQAGWSGVRWRNLTGG
                     IVALHAATR"
     gene            complement(6983..8107)
                     /locus_tag="VF874_15245"
     CDS             complement(6983..8107)
                     /locus_tag="VF874_15245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9177369.1"
                     /translation="MRVAIVAESFLPNVNGVSNSVLRILEHLNHTGHDAIVVAPDNPR
                     RQEGAATQHDGVPVHRMPSRMFPKITSLPLGVPRPRMVSILRSFDPDVVHLASPALLG
                     YGGLQAARLLGVPTVAVFQTDVPGFATSYGMSATSRVAWAWFRHLHRHADRTLAPSSS
                     AMSSLVAQRIPRVYLWARGVDIAGFAPSARDQALRRAWSPAGKPIVGFVGRLAPEKHV
                     ERLAALAGGDVQLVVVGDGVDRVKLEKTLPTAVFTDALYGAELAAAYASMDVFVHPGE
                     HETFCQAVQEALASGVPVIAPNAGGPRDLVMPGRTGLLLDVAAFERQLPGAVMHLLQA
                     RSHYAPAARRSVLQRTWPVICDELLGHYEAVLQRSQAKAA"
     gene            8354..8737
                     /locus_tag="VF874_15250"
     CDS             8354..8737
                     /locus_tag="VF874_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177370.1"
                     /translation="MRDGADELSTSDDENANRLRGLLSSAIASMLRTCIDHSDAARIY
                     WQMVTRKHRERWAQGDIDGIIPVLSELLPWIREAGIDLSANTDVAALCKDPSFEKMRN
                     WLRTTGCDPKIFDLFRQLAGPTDRP"
     gene            complement(8768..9370)
                     /locus_tag="VF874_15255"
     CDS             complement(8768..9370)
                     /locus_tag="VF874_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX9177371.1"
                     /translation="MLAPPASAQPVAPPDFVALSDVDPTILQEIRYFTPHNFVGDPID
                     GYQQPMCILTREAAEGLKRAQQAFVEQGYTLKVYDCYRPQRAVDEFVAWGENPLADRS
                     GVGQQRDNRGRVSDQSSRQRRSASLASRIADASTRTPVASTRARARAITSLTDGLAAV
                     STSRPRRNSCSDLPDRAARAASSSRTPSGISRTVMATMPA"
BASE COUNT         1590 a         3214 c         3183 g         1562 t
ORIGIN      
        1 gtatcacggt ctggccgccg agacgccggg gcacgagatc cacggcgcag gtttctgagt
       61 tccgctactt gcgccgagag tgaagccagg gctgtgatcc ggctcagaac acgacctgga
      121 attcagtttc ggcgcaaagg cgttaggtgc cgctggccct ttgcggccgg cccggcttgc
      181 gctcccaact gtcggcgaaa ttccgaatgg cacttccgtc ccaaacgggg cctccacgga
      241 gatcggccag cggggcaggg aaagcatcaa tcgtgcgcag ttgatgcact cgttggcgcg
      301 agatgcctaa ctcgtcggcg atctcggaag ccgacatcag ctccggcaga cttggctgct
      361 ctgatcgacg gaatagctcc cctcggtgat gatctcgaca ccaaccggct cactgcgaac
      421 cacgctcgag acggcttcgg ctagtttgcg acccgccttc cacaaatcca tgtggtcggg
      481 cgcatgaact acgacgtcga tgccgcggcc ggggacacgc gagaccatgg catcgaaccg
      541 ggcgagctga tcctcccacc gatccatgtc gtccatcgac ggatcgaggt cgtaggaaag
      601 cgtgatgacc caggattagc cgcggaatgg cattccgggt gatttgagcc ggaccacagc
      661 cgtagctccg gtttccgcgc gaagatgtta ggccgtgaag ctgcccgccg ggctgagcac
      721 gaagccgtgc tgggtcgcgc tgttgacgca caccgtggct ccgcccgcgt cgatcgcgca
      781 gctgacattg cggtagctga tccgtgtctg cggcggcagc ggcttcaccg ggcccgtcac
      841 cgcgaacaac ggcgccgccg tgttggcgaa ccctggctcc tcgccgccga ccccgctgac
      901 caggttcgcg ccctccggcg cggccgggat cggaccgctg catccgtagc tgttgcgccc
      961 cttgtccagc acgcacgtca gcccgtcggg cgtcgcgaac gcgtaccagg taccgtccat
     1021 caccgagaac tcggcgggag agatcggcgg atagacgttg atgttcggca gcacgggcgc
     1081 ttcgtcggcg cccgcgacgg gcgaggtcgc catcaccagc accgttccca cggcaccggc
     1141 caggccggtc agcactctgc tcagcacgga atcacactac gacgggtgca ggccgggcgc
     1201 tcggacgacc atcggcaccg cggggaagta gctcgccatc tccgagcgtg acttgcgcag
     1261 cgcggccgtt ccgtagccct tcttgcggtg ctcggggtgg atccacaccc tgacgttcac
     1321 ctcgccgcct gccaattcac cgaagaccat cccgaccttt tccgtaccag agacggcgac
     1381 aagccacacc gcgtcctcgt cgtcgattcg tcccagtcca gaccggatct cgtcatcaag
     1441 agagccagcc ggtccgccgg agccgtcgcc cgccacatgc atgtcggcgg ttcgcgcgga
     1501 gaagatgtcg cggtcgtcgg cgccggcgaa cagccggagc gtcacgccct ccccggtgct
     1561 cgccggccgc tcgccgagag tgaacgtcag ctggttgttc aggtcgtcga gctcgtcggc
     1621 gatcctgcgc cgcgactctt tggtgaattt gtcgaacgac atgccgatca cggcttcggc
     1681 gcccagccgc gaggtgccga gaagcgtcgc gatcgcctcg acagcggcct cgcgatcttc
     1741 ggcttcgacg accgcgtcgg ccacgtcgtg acgacgctcg agcgccgtga cgagggcgtt
     1801 gtcgatctcc cgcctgacag cggcgcggtc ctggtccgtc atgggcctca gcctagtcgc
     1861 gaagccacga ccgccagaaa tcgtcgcgcg ccggctctcg gccactgagc ttttgcacga
     1921 cccactgatt catcgacacg ccctgctcgg tggcttcgac ggtaaggcgg gcatgcaatg
     1981 ccagcgacgt gcggaccacg aacttgccgc tgtacttgcg ttccatcagc ggcggcggca
     2041 actcttgacc gcgagcggcc atgtcgtcga ccgcctcctc aaccgctgct actgcctcgc
     2101 gagcggttgg ggcgctgcgg gagagccatg aagttcaacg cacagaccga cgtagtgacc
     2161 ggcctcgggt gagtactcgg cgcggtaggt gtatgtaatg gcgaaaaaag tgatagcaca
     2221 tacgctatcg ctttgtcaga agtgtctaac gcaccaaaag ccgggcgcca acggcaccgg
     2281 gaccgcggct cagtacgacc cgcgggccat cgcctccagc cgcgcgatcc gctcggcgat
     2341 cggcgggtgc gtcgagaaca accccaccat ccgctcgcca gagcggaacg ggctcgcgat
     2401 catcaggtgc gactcgctgg cgagctttgg ctccggcggc agcggcgcct gctgcacacc
     2461 ggccgagatc tttcgcagcg ccgacgccag cgccagcgga tcgcccgtga gctcggcgcc
     2521 cgactggtcg gcttggtatt cacgggaccg cgacaccgct aaccgaacga ccgtcgcagc
     2581 gatcggcgcc agcagcgaaa tcagcagcag cgcaaaggga ttggcgtctc ggttgccgcc
     2641 ggtccagctc atgatcatcg ccaggttcgc caaaccggag atcaccgcgg ccagcgcgcc
     2701 ggccacacac gagatcagaa tgtcgcggtt gtagacgtgc gacagctcat ggccgaggac
     2761 cgtccgcagc tcacgctcgt tgagaatcgc cagaatcccc gtggtgcagc acaccgccgc
     2821 attgcgcgga ttacggcccg tagcaaacgc attcgggttc gcggtgtcac tgatgtacag
     2881 ccgcggcatc ggctggtgcg ccaccgtcgc cagctcccgg acgattcggt gcatcgtcgg
     2941 ctcctgcagc tcggtcaccg gctgcgcgtg catcgcccgc agcgccagct tgtcgctgtt
     3001 gaagtacacc cagacgatca tgccgaccgc gaacaggacc gacaaaatca ggatgttcct
     3061 gccgaacagc gacccgacga acacgatcag cccgaacatc agcccgagaa gcagcaccgt
     3121 cttgatgccg ttgccccatc ggtgcccgac catcaccaac tcctcttgta cgcgaacgaa
     3181 tcgttaagag aacgctgagg gacgccgccg ggttccgtca accgtgacgc gaaatcgtgt
     3241 agtcgacgag cgtggccaac gcctggcgtc ccgcgccgtc gggcagcccg gccagctcgt
     3301 cgcgggcctg cgccgcatac cgcgcgacgg tgtccttggc ctcggccatt cccggcgacg
     3361 cccgcagcag cgccagcgcc tcggccacgt gggcgtcgtc gtccatcggg ccggccagca
     3421 gctcgcgcag ccgctccgcg tcgggcccgc tttcgcgcag cgcgtacagc actggcaatg
     3481 tgtgcacgcc ttcgcgcagg tcggtgcctg ggatcttgcc cgattcgtcg gagtcgctgg
     3541 cgatgtcgat gatgtcgtcg gagatctgga acgcggtgcc gacgatgccg ccgagccgcc
     3601 tcagccgctg cacgtccgcc tcggccgcgc cggagaacgt cccaccgaac tggcccgatg
     3661 ccgcgatcag cgaacccgtc ttctcgtgca ccacccgcag atagtggtcg accgagtcga
     3721 cgtgctgggc agcgccgcgc gtctcgcgca tctggcccgt caccaactcg gcgaatgtct
     3781 cggcgatcac ccgcaccgcg tccggaccca gtttgctgac cagccgcgac gccgtcgcga
     3841 acaggtagtc gccggccaaa atcgcgatat tgttgctcca gcgcgcgttc gcgctgggcg
     3901 cgccgcggcg gacctgcgcc tcgtccatca cgtcgtcgtg gtacagcgtg gccaggtgca
     3961 ccagctcgat gaccgacccc gcgaccgcca cctgccacgc gtcggggttc gggcccagct
     4021 gcgcgctcag caccgtgaac agcgggcgga accgcttgcc gccggcatcg aacaggtgca
     4081 gcaccgcatc gcggatgatc tcgtcgccct tgccgagctc ctcgctgatc agcgtctcga
     4141 tgcgggccac gccgtcgcgg acgctcgccg cgaaggcggg atcgcctagc tcgacccccg
     4201 ccaccacggt cgctggcgtc ttcacgcgtc caacatactg gtgagcatgg atgccagcgc
     4261 ggacgtggtg gtcgtcggcg ccggtccggc cgggtcggcg gcggccgcgt gggcggcgtg
     4321 ggccgggcgc gacgtactgg tgatcgatgc ggcgtccttt ccccgggaca agccgtgcgg
     4381 cgacgggttg acgccgcgtg ccgttgccga gttgcagcgg ctgggcctgg gcgactggct
     4441 cgacgcgcac atcaagcacc gcggactgcg actgaccggg ttcggcggcg aggtcgagat
     4501 cgactggccg ggtccgtcat ttcctgccta tggatctgcg gtgccgcgga cggagctcga
     4561 cgagcgggtg cgtcaggtgg ccgtcgacgc ggagtgccgg atgctgctgg gctcgaaagt
     4621 ggttgaggct ccccgtgatc cgcgtgggcg ggtgacgtcg ttgcggctga gttcggggtc
     4681 atcggtcgcc tgccgggagg tgatcgtcgc cgacggcgcg cgctcgccgc tgggccgggc
     4741 gctgggccgc gagtggcacc agcagacggt gtacggcgtg gcggtgcggg ggtatctttc
     4801 gacgccgcgt gcctctgacc cctggttgtc gacgaacctg gagttgagga cccgtcacgg
     4861 ccacctgatg cccgggtacg gctggatttt cccgctgggc aacggcgagg tgaacatcgg
     4921 cgtcggcgcg ctggccacgc agaagcgccc ggccgacgtg gcgttgcgga agctgatcgg
     4981 ccactacgcc gacctgcggc ggcacgagtg ggacttcgac gggccgccgc gggcggtgtc
     5041 gtcggcgctg ctgccaatgg gtggggcggt gtcgggcgtg gccgggccga actggatgct
     5101 gatcggggat gccgccgccg gtgtgaaccc gctgaacggc gagggcatcg actacgggct
     5161 ggagatgggc cggttcgcgg cggaaatgct tggcagcggc gatctttcac aggcgtggcc
     5221 ggcgctgctg caggagcact acgctcaagc gttctctatt gcgcggcggc tggcgtggct
     5281 gctgaccgtg ccatggctgt tgccgacggt cggcccgttc gcgatgcggt cggtggcgtt
     5341 catgcagacg gtcgtgcggg tgatgggcaa cctcgtcgtc gacgaggacg tcgaccgggt
     5401 ggcgcggtta tggcgccggc tgggcgcggg gtcgcggttc gtcgaccgcc gcccgccgtt
     5461 cagctgaggt tgccaacggg cgcactcccg gtggccagct gggagcacat catgtgccac
     5521 cttggtgcgg tggagcagat cggaatccgt gaactgcgcc agcacgcaag tcgctgggtc
     5581 gccaaggcga aagcgggcgc agccattctc atcactgacc gggggcggcc ggtggcccgg
     5641 cttgtcccga tcaccgctgc cgaacacgcg cgcgaaacgt tgatcgccaa aggactcctt
     5701 caaccggcgc cccgaccacg accgaagttc ggcgttgagc aactcatcga cggacctccg
     5761 ctgaccgaga tccttgatga catgcgctcc gatcgatgat ctacattgac acgtctgcgt
     5821 tggtgaaact cgttgtggcg gagcaggaat catctcactt gatagactgg ctcaacagcc
     5881 gtcaagatca accgctcgtc acgagcgtcg tcggccacat cgagttggtg cgtgttgcgg
     5941 gtcgtgttgg agaggtcgaa acggctgccg cacagcactt ggccgcgacg gtcgacacgt
     6001 taacccttag cgattccatt gcgaactacg cggcgacctt gtcgcttgcc acgctacgca
     6061 ccctcgacgc gatacatctg gccacggcac acgagtatcg acaagacctg tcaacgctgt
     6121 gcgcctacga ccatcggctc atcgaggcag cccgatctca ccggctaccg gtgacgtctc
     6181 cgggagcagg gtgacgagcg ccgcgagcgt gcgctcttgt atgcctaaca gcggcgtgtc
     6241 ggagtacaga cccgcacgct cgcggcaaag gtgggggcaa aggggttagc gggtggcggc
     6301 gtggagggcg acgatgccgc ccgtgaggtt gcgccaccgc accccagacc agcccgcttg
     6361 ctggatgaga cgggcaagct cggcctggtc gggccacgcg cggatcgact cggcgaggta
     6421 gacgtacgcc tcggggttcg acgccaccgc gcgcgcgacc cgcggcagcg cctgcatcag
     6481 gtactccttg tacaccgtgc cgaacagcgg gttcgccggc gtcgagaatt cacacaccac
     6541 cagccgcccg cccggcctcg tgactcgcgc catctcccgc agcgccgcct cgcggtccac
     6601 cacgttgcgc agcccgaagc tgatcgtcac cgcgtcgaac gtctgatccg agaacggcag
     6661 ccgcgtcgca tcgcctgcca ccttcggcac agcccgcgcc ttgcctgcca gcagcatgcc
     6721 caccgagaag tcggcggcca cacaccacgc gcccgagcgc gccagttccg aagtggacac
     6781 cgcggtgccg gccgccagat ccagcacgcg atctccggcg tcgacgtcca gcgccttgcg
     6841 cgtcgcgcgt cgccagaacc ggtcctgccc caacgacagc acggtgttgg tgatgtcgta
     6901 gcggcgcgcg accgcgtcga acatcgacgc gacctcgtgc ggatccttgt ccagtgtcgc
     6961 ccggctcacc acgccgaagc tatcaggcgg ccttggcctg actgcgttgt agcacggcct
     7021 cgtaatggcc gagcagctcg tcgcagatga ccggccacgt gcgctgcagc acgctgcgcc
     7081 gcgcggccgg cgcgtagtgc gaccgcgcct gaagtaaatg cattacggcg ccgggcagtt
     7141 gacgttcgaa ggccgcgacg tcgagcagaa gacccgtgcg gccgggcatc accaagtcac
     7201 gcgggccacc cgcgttgggt gcgatcaccg gcacgcccga cgccagcgcc tcctgcacgg
     7261 cctggcagaa cgtctcgtgc tcacccgggt gcacgaacac gtccatgctc gcgtacgcgg
     7321 ctgccagctc ggcgccgtag agcgcgtccg tgaaaaccgc tgtgggcaaa gtcttttcaa
     7381 gtttgacgcg gtcgactccg tcgccgacga ccaccagctg cacatctcct ccggccaacg
     7441 ccgcaagccg ctcgacgtgc ttctccggcg ccagcctgcc gacgaacccc acgatcggct
     7501 tgccggcggg cgaccacgcc cgccgcagtg cctgatcacg cgccgacggc gcgaatcccg
     7561 cgatgtcgac accgcgggcc cataggtata cccgcggaat acgctgcgcc acaagagaag
     7621 acatcgccga cgacgacggc gccagcgtgc ggtcggcgtg ccggtgcagg tgccggaacc
     7681 acgcccacgc cactcgggag gtagcgctca tcccgtagct cgtcgcgaac ccggggacgt
     7741 cggtctggaa caccgcgacc gttggcaccc caagcaagcg cgccgcctgc agcccgccgt
     7801 atccgagcag cgccggcgac gccagatgca cgacgtccgg atcgaacgag cgcaggattg
     7861 aaaccatgcg cggccgcggc acaccgagcg gcaacgacgt gatcttcggg aacatccgcg
     7921 acggcatccg atgcaccggc accccatcgt gctgggtggc ggccccctcc tgccggcgcg
     7981 ggttgtccgg ggcgacgacg atcgcgtcgt gaccggtgtg gttcaggtgc tcgaggatgc
     8041 gcagcaccga attcgagacg ccgttgacgt tcggcaggaa cgactcggcg acgatcgcaa
     8101 ctcgcacgcg gccagcgtgc cgggcgtccc tatcgtcgag gttgccgaca ggaatacacg
     8161 acacgaactg tcgtcggctg cggtgtgatt acggacgtcg agcgactcga gctgaatgca
     8221 tagtccaagg aaatactcat gaaaacgtta tgtgcgccgc ggcgatctga ggtaacgtcg
     8281 gttttcaaga atttgggagc aacgggggcc acctttgcga gttacgttcg cggaacgccg
     8341 cgtcgtgcgg attatgcgcg atggcgccga cgaattatcg accagcgacg acgagaacgc
     8401 caaccgcctg agggggttgc tgagcagcgc gattgccagc atgctgcgaa cgtgcatcga
     8461 ccacagcgac gcggcgcgta tttattggca gatggtcacc aggaaacatc gggaacggtg
     8521 ggcgcaaggt gacatcgacg ggataatccc cgttctttca gagttattgc cgtggattcg
     8581 ggaagccggt atagacctct ccgcaaatac tgatgtggcc gcgttgtgca aagatccgtc
     8641 gttcgaaaag atgcgcaact ggcttcgtac gaccggttgc gacccgaaga ttttcgacct
     8701 ctttcgccaa ctcgctggcc ccaccgaccg gccctgactg gtctgcattc tgactgtatt
     8761 gcagctatta cgcaggcatg gttgccatca cagtgcggga tatccctgat ggcgtccgcg
     8821 acgagctcgc ggcgcgcgcg gcgcggtccg gcaagtcgct gcaggagttc ctgcggggtc
     8881 tgctcgtcga cacggcggca aggccatccg tcaatgatgt gattgctcgc gcgcgagcac
     8941 gcgtagaagc gacgggggtg cgcgtggatg cgtccgcgat cctcgaagcc agagacgccg
     9001 accgtcgttg acgcgacgat tggtcgctga cgcgtccgcg attgtcgcgt tgctgaccga
     9061 ctccggaccg gtcggccagt gggttttcgc cccatgccac gaactcgtcg acggcgcgct
     9121 gcggccggta gcagtcgtac accttcagcg tgtagccctg ctcgacgaac gcctgctgtg
     9181 cgcgcttcag cccctcggcc gcctcgcggg tgaggatgca catcggctgc tgatagccgt
     9241 cgatcgggtc gccgacgaag ttgtgcggcg tgaagtagcg gatctcctgc aggatcgtcg
     9301 ggtcgacgtc gcttagtgcg acgaagtcgg gcggcgccac cggttgcgct gacgccggag
     9361 gcgcgagcat aacggcgagc aaggccgcgg cgacgagtcc gatactgcgc cgagagtgaa
     9421 gccatggctg ctgaatcagg gttttcacga cctggaattc tatttcgcgg aagcggaagt
     9481 cgcggcgata gggtcggaca tgcgctggct gaggatggcc gtggtggtcg gcgtcatgct
     9541 caccggcga
//
LOCUS       DASWBF010000410         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_698927,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000410 DASWBF010000000
VERSION     DASWBF010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698927"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2662)
                     /gene="mfd"
                     /locus_tag="VF874_15260"
     CDS             complement(<1..>2662)
                     /gene="mfd"
                     /locus_tag="VF874_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9177372.1"
                     /translation="GPIGSVGDMLAKLADGIPVDGMEALMPVLGAGQLTLLTNYLPKG
                     TPLLVCDPEKVRARAADLIKTGQEFLEASWSTAALGGDAPVDLEQLGASGFLELDDVR
                     VAAVEAEHSWWTLSQLSDPDAVRLAIQAAPSARGHQHSVEEIFAMLRVHVSTGGTAAI
                     VAPGTGTAHRVVEQLRDADVAAVCLDPGDTPTPGVVGVLRGPLHDGIVLPESNLVVIT
                     ETDLTGSRVAVTEGKRLAAKRRNVVDPLALTKGDLVVHDQHGIGRFLEMVERTVGGAR
                     REYLVLEYASGRRGGQDRLYVPMDSLDQLSRYVGGEAPTLSRLGGSDWANTKTKARRA
                     VREIAQELVALYAKRQASPGFVFSPDTPWQAEMEDAFGFTETVDQLTAITEVKSDMEK
                     PIPMDRVICGDVGYGKTEIAVRAAFKAVQDGKQVAVLVPTTLLADQHLQTFAERMAGF
                     PVRVKALSRFIGTAESKAVLDGMADGTVDIVIGTHRLLQTGVRWKDLGLVVVDEEQRF
                     GVEHKEHIKSLRTHVDVLTMSATPIPRTLEMSLAGIREMSTILTPPEERYPVLTYVGP
                     HDDKQIAAALRRELLRDGQAFYIHNRVSSIDAAAARLQALVPEARVVVAHGQMPEDLL
                     ERTVEGFWNREYDILVCTTIVETGLDISNANTLIVERADTFGLSQLHQLRGRVGRSRE
                     RGYAYFVYPREQPMTETAYDRLATIAQNNELGAGMAVAMKDLEIRGAGNVLGVEQSGH
                     VAGVGFDLYVRLVGEAVEAYRAAADGQAIAAPEEPKEVRIDLPVDAHLPPDYIPSDRL
                     RLEAYRRLAAAGDDAAVAAVVDELTDRYGPLPEQARRLVAVARLRLLARGYGVTEVTT
                     TGTSLRVSPLTLLDSQQVRLKLLYPSASYRA"
BASE COUNT          369 a          952 c          923 g          418 t
ORIGIN      
        1 gcgcggtagc tggccgacgg gtacagcagc ttaagccgca cctgctgcga gtccaacagg
       61 gtgagcggcg acacccgcag cgacgtgccg gtcgtggtca cctcggtcac gccgtacccg
      121 cgcgcgagca accgcagccg cgccacggcc accagccgcc gcgcctgctc cggcagcggc
      181 ccgtagcggt cggtcagctc atcgacaaca gcggcgacgg cggcgtcgtc gcccgctgcc
      241 gccagccggc ggtaggcctc cagccgcagc cggtcgctgg ggatgtagtc cggcggcaag
      301 tgcgcgtcga ccggcagatc gatgcgcacc tccttgggct cctcgggcgc ggcgatggcc
      361 tggccgtccg cggcggcgcg gtacgcctcg acggcctcgc cgaccaggcg cacgtacagg
      421 tcgaaaccca ctcccgcgac gtgcccggac tgctccaccc cgagcacgtt gccggcgccg
      481 cggatctcga ggtccttcat cgcgacggcc atgcccgcgc cgagctcgtt gttctgcgcg
      541 atcgtggcga gccggtcgta tgcggtctcg gtcatcggct gctcgcgggg atagacgaag
      601 taggcgtagc cgcgctcgcg gctgcgaccc acccgaccgc ggagctggtg cagttgggac
      661 agcccgaacg tgtcggcgcg ctcgacgatc agcgtgttcg cattggagat gtccagcccg
      721 gtctcgacga tcgtggtgca caccaagatg tcgtactcgc ggttccagaa gccctcgacc
      781 gtgcgctcca gcaggtcctc gggcatctgg ccgtgcgcga cgaccacccg cgcctccggc
      841 accagcgcct gcaggcgggc ggcggcggcg tcgatggagc tgacccggtt gtggatgtag
      901 aacgcctgtc catcgcgcag cagctcgcgc cgaagggccg cggcgatctg cttgtcgtcg
      961 tgcgggccga cgtaggtgag caccgggtag cgctcctcgg gcggcgtcag gatcgtcgac
     1021 atctcgcgga tgccggccag gctcatctcc agcgtgcgcg gaatcggggt ggcgctcatc
     1081 gtcagtacgt cgacgtgggt gcgcaggctc ttgatgtgct ccttgtgctc gacgccgaac
     1141 cgctgctcct cgtcgaccac caccagtccg aggtccttcc accgcacgcc ggtctgcagc
     1201 agccggtgag tgccgatcac gatgtcgacg gtgccgtcgg ccatgccgtc gagcaccgct
     1261 ttcgactcgg cggtgccaat gaaccgcgac agcgctttca cgcgcaccgg gaatccggcc
     1321 atccgctcgg cgaacgtctg caggtgctgg tcggccagca gcgtcgtcgg caccagcacc
     1381 gcgacctgct tgccgtcctg caccgccttg aacgccgcgc gcaccgcgat ctcggttttg
     1441 ccgtagccga cgtcgccgca gatcacccgg tccatcggga tcggcttctc catgtcggac
     1501 ttgacctcgg tgatcgcggt caactggtcg acggtctcgg tgaacccgaa cgcgtcctcc
     1561 atctccgcct gccatggggt gtcggggctg aacacaaacc cgggcgacgc ctgccgcttg
     1621 gcgtagagcg cgacgagctc ctgcgcgatc tcgcgcacgg ctctgcgggc cttggtcttg
     1681 gtgttggccc agtcgctgcc gcccagcctg gacagcgtcg gcgcctcgcc gccgacgtag
     1741 cgcgacagct ggtcgaggga gtccatcggc acgtagaggc ggtcctgacc accccgccgg
     1801 ccggaggcgt actccagcac caggtactcg cgacgcgcac cgccgaccgt gcgctcgacc
     1861 atttcgagga accggccgat gccgtgctgg tcgtgcacga cgaggtcgcc cttggtgagc
     1921 gcgagcgggt cgacgacgtt gcgtcttttg gcagccagcc gcttgccttc ggtgaccgcg
     1981 acccggctgc cggtcaggtc ggtttcggtg atgacgacga ggttgctttc gggcagcacg
     2041 atcccgtcgt gcagcgggcc tctgaggacg ccgaccacgc cgggggtcgg cgtgtcgccc
     2101 gggtcgaggc agacggctgc gacgtcggcg tcgcggagct gctcgacgac ccggtgcgcg
     2161 gtgccggtgc ccggcgcgac gatggccgcg gtgccgccgg tgctgacgtg gacgcgcagc
     2221 atcgcgaaga tctcctcgac gctgtgctgg tgaccgcgcg ccgacggtgc cgcctggatt
     2281 gcgagccgta cggcgtcggg gtccgacagc tggctcaacg tccaccagga atgctcggcc
     2341 tcgactgccg cgacgcgcac gtcgtcaagc tccaggaacc ccgacgcgcc gagctgctcg
     2401 aggtcgacag gcgcgtcacc gcccagtgcg gccgtcgacc acgacgcctc gaggaactcc
     2461 tgtccggtct tgatcagatc ggccgcgcga gcgcgaacct tctcggggtc gcagaccagc
     2521 agcggcgtgc ccttcggcaa gtaattggtg agcagcgtga gctggccagc gcccagcacc
     2581 ggcatcagcg cctccatccc gtcgaccggg atgccgtcgg cgagcttggc cagcatgtcg
     2641 ccgacgctgc cgatcgggcc gc
//
LOCUS       DASWBF010000411         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_48284, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000411 DASWBF010000000
VERSION     DASWBF010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48284"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VF874_15265"
     CDS             <1..466
                     /locus_tag="VF874_15265"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX9177373.1"
                     /translation="RQARGQSEIPPIAVVTASGALDLGSRLFTNTETPPLVFTTTASF
                     VATRDRLHGQAEVLDASTSDPIVVDPAAVLAELAQRGLYRLLCEGGPTLLGDVVELAL
                     LDELALTVAPMLVGGASPRVVTSHAPTTTELRTEHILSDAEGYLYLRYARRR"
     gene            490..2055
                     /locus_tag="VF874_15270"
     CDS             490..2055
                     /locus_tag="VF874_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9177374.1"
                     /translation="MPFPSPLRVAAALVATISVLAGCAPGLAANPKYATDSGQEPQGN
                     PPTTPPPNGPPPIEAPKSDLSWRDCTSRLFSEVSIAPPVGVTLDCATFDADLDPIDNA
                     TGTVTIGVVRARSPQTPPDAGPLVITTGSDLPSSIQLPVWLTRSGTDVLKSRPIVSID
                     RRGIGLSGALDCRDSFDRQEMADQAQFESGDDPVANLGEIVVTATTNCTDTISPGDSA
                     YDNRHAAEDIERLRTTWDVPGLALLGIGNGAQVALAYAGRHPDKVARLILDSPIPPAI
                     NAEGAEEHQVKGEQAGLEAFVAQCAATNCPLAPDPTSAVDAIIAAARSGRGPGGASVA
                     AVTSAISTALAFPRGDQAAATNSLAAALAAARSGDDNQLNNLINQAQSLRETDGQFVN
                     QCSNSLNRPTPDRVRELLVEWGKLYPQFGAVGALDLVKCLNWPSGTAPPDPKNLKVNV
                     LLMGGQNDPIVGSEGVAAVSATVLNAGVASKRVMWQGIGHGVSTYSSCAVPPMMGYLD
                     SGNLPSTDTYCPA"
     gene            2057..>3133
                     /locus_tag="VF874_15275"
     CDS             2057..>3133
                     /locus_tag="VF874_15275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX9177375.1"
                     /translation="MAFAVRYGALVALPDSVLNVFRPRTSPPTASQVLRTILWPLAIL
                     FIIHRSYVLATNGYITDDYGPVYRAVSNFRRGLDIYNEHFDYVDPHYLYPPGGTLLMA
                     PFGYLPVDASRYWFIFFNTVAMIFAAYLLIRLFKFTVTSVAFPALLAAMFCTEAVVNT
                     LVFANINGIILLLEVLFFRWMLDGKVGHQWWAGVAIGLSLVVKPLLAPLLLMPLLNRQ
                     WRVLGTAIAVPVAFNVAAFPLVADPMKFYKQTLPYILLKTRDYFNSSIAGNGLYYGLP
                     MWLIVLLRIIFGLLAVGALWLLYRYYRTRDPLFWFCTSSGVLLIASWLVLSLGQAYYS
                     MALFPFLMTVVLRNSVIRNWPAWLA"
BASE COUNT          481 a         1088 c         1022 g          542 t
ORIGIN      
        1 tcggcaggcg cgcggccagt ccgagattcc gccgatcgct gtggtgacgg cgtcgggtgc
       61 tctcgacctc ggcagccggc tgttcacgaa taccgaaacg ccgccgctgg tcttcaccac
      121 cacggcgtcg ttcgtcgcca cccgcgaccg gctgcacggg caggctgagg tgctcgacgc
      181 ctcgaccagc gatccgatcg ttgtcgaccc ggctgcggtg ctggccgagc tggcgcagcg
      241 cgggctgtat cggctgctgt gcgagggcgg accgacgctg ctcggcgacg tcgtggagct
      301 ggccttgctc gacgagctcg cgttgaccgt cgcaccgatg ctggtgggcg gcgcatcacc
      361 gcgtgtggtg accagccacg cgccgacgac caccgagctc cgtaccgagc acatcctgtc
      421 cgacgcggag ggatacctgt acttgcgcta cgcccggcgc aggtgacgcc gctcgctact
      481 ctgggcagca tgccgtttcc aagccccctg cgagtcgccg ctgcgctggt cgcgacgatc
      541 tcggtgctgg ccggctgcgc gccggggctg gcggccaacc cgaaatacgc caccgactcc
      601 ggccaggaac cgcaaggcaa cccgccgaca acgccgcccc cgaacggacc gccgccgatc
      661 gaggcgccga agagcgatct gtcctggcgg gactgcacgt ctcggctgtt ctccgaggtg
      721 tcgatcgctc cgccggtcgg tgtgacactg gactgcgcca cgttcgacgc tgacctcgac
      781 ccgatcgaca acgccaccgg gaccgtcacc atcggtgtgg tgcgcgcgcg ctcgccgcag
      841 acgccgcctg acgccgggcc gctggtcatc accaccggat cggatctacc gtcgtcgatc
      901 cagttgccgg tgtggctgac gcgctcgggc accgacgtgc tgaagagccg cccgatcgtc
      961 tcgatcgacc gccgcggcat cgggctgtcc ggcgcactgg actgccgcga ctcgttcgac
     1021 cggcaggaga tggccgatca ggcccagttc gagtccggcg acgatccggt ggccaacctc
     1081 ggcgagatcg tcgtcaccgc gacaacgaac tgcaccgaca ccatctcccc cggcgattcg
     1141 gcgtacgaca accggcatgc cgccgaggac atcgagcggc tgcgcacgac gtgggacgtg
     1201 cccgggttgg cgctgctggg catcggcaac ggtgctcagg tcgcgctcgc ctacgccgga
     1261 cggcacccgg acaaggtggc ccgactgatc ctggactccc cgatcccgcc ggcgatcaac
     1321 gccgagggtg ccgaggagca ccaagtcaag ggcgagcagg cagggctgga ggccttcgtc
     1381 gcgcagtgcg cggccaccaa ctgcccactc gccccggacc cgacgtcggc ggtcgacgcg
     1441 atcatcgcgg ccgcacggtc ggggcgcggt ccgggcggcg cgtcggtggc ggcagtgacg
     1501 tcggcgatca gcacggcgct agccttcccg cgcggtgacc aggcggcggc gacgaacagc
     1561 ctcgcggccg cgctggccgc ggcgcgctcg ggtgacgaca accagctgaa caacctgatc
     1621 aaccaggcgc agtcgctgcg tgagaccgac ggacagttcg tcaaccaatg cagcaactcg
     1681 ctgaaccgtc cgacacccga ccgggtccgc gaacttctcg tcgaatgggg caagctgtac
     1741 ccgcagttcg gcgcggtcgg agcgctcgac ctcgtcaagt gcctcaactg gcccagcggc
     1801 accgcgccgc ccgacccgaa aaacctcaag gtcaacgtgc tgctgatggg cggacagaac
     1861 gacccgatcg tcggcagcga aggcgtcgca gccgtgtcgg cgaccgtgct caacgccgga
     1921 gtggccagca agcgggtgat gtggcagggc atcgggcacg gtgtgagcac atactcgtcg
     1981 tgcgcggtgc cgccgatgat gggctacctg gacagcggaa accttccgtc caccgacacg
     2041 tactgcccag cctgatctgg cgtttgccgt caggtacggt gcgctcgtgg cgctccccga
     2101 ctccgtcttg aacgtcttcc ggccccgcac cagccctccg accgcatcac aggtgttgcg
     2161 cacgatcctg tggccgctgg cgatcctgtt catcatccac cgcagctacg tgctggccac
     2221 caacggctac atcaccgacg actacgggcc ggtgtaccgc gccgtgtcca atttcaggcg
     2281 cggcctggac atctacaacg aacatttcga ctacgtcgac ccgcactacc tgtacccacc
     2341 gggcggcacg ttgctgatgg cgccgttcgg ctacctgccg gtggacgcgt cgcggtactg
     2401 gttcatcttc ttcaacaccg tcgcgatgat cttcgccgcg tatctactga tacggctgtt
     2461 caagttcacc gtcacctccg tcgcgttccc cgcgctgctg gcggcgatgt tctgcaccga
     2521 agccgtcgtc aacacattgg ttttcgcgaa catcaacggc atcatcctgc tgctcgaggt
     2581 gctgttcttc cggtggatgc tcgacgggaa ggtcggtcat cagtggtggg ccggcgtcgc
     2641 gatcgggttg tcgctggtgg tcaagccgct gttagcgccg ctgctgctga tgccgctgct
     2701 caaccggcaa tggcgcgtac tcggcaccgc gatcgcggtg ccggtggcat tcaacgtggc
     2761 cgcgtttccg ttggtcgccg acccgatgaa attctacaag cagacgctgc cgtacatcct
     2821 gctgaagact cgcgattact tcaacagctc gatcgccggc aacgggctct actacggcct
     2881 gccgatgtgg ctgattgtct tgctgcgcat aatctttggc ttattggccg tgggcgcgtt
     2941 gtggctgctg taccgctact accgcacccg cgacccgttg ttctggttct gcacctcgtc
     3001 gggcgtactg ctgatcgcgt cgtggctggt gctgtcgctg gggcaggcgt actactcgat
     3061 ggcactgttc ccgttcttga tgacggtggt gctgcgcaac tcggtgatcc gcaactggcc
     3121 ggcgtggctg gcg
//
LOCUS       DASWBF010000412         6126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_433449,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000412 DASWBF010000000
VERSION     DASWBF010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433449"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VF874_15280"
     CDS             <1..251
                     /locus_tag="VF874_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX9177376.1"
                     /translation="HAKLLIADNQHALVGSMNIDRSAFDLRRELGITIADPDVVSRLK
                     EVFSSDWDLSHHYDPPDPLDPSKHTEDDFPHDHDLMHE"
     gene            244..807
                     /locus_tag="VF874_15285"
     CDS             244..807
                     /locus_tag="VF874_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX9177377.1"
                     /translation="MSEIDPPREKVSLFELAWTFNHIALASFGGGLSAWSREVLVVEK
                     NWMGEEEFLSALTMCRILPGANQVNMAVFVGTKMAGIPGAVVAVFGLCLMPLVFILTV
                     AYIYFRFKEVPAVKGSLHGASAAAVALTLAMVIKTGQKCLTGLVPVVLFAASFVLNGL
                     LRWHLPVTLAILAPLSLIWAWPRKTTT"
     gene            804..1349
                     /locus_tag="VF874_15290"
     CDS             804..1349
                     /locus_tag="VF874_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX9177378.1"
                     /translation="MNTYVQLAILFTSLSLLSIGGGNAVLPEMHTKAVRGEHWLTDSQ
                     FADVFSISQAAPGPSILIVALIGYGAGLSVGGVPGAILGGVIATVSMITPAGILVYVV
                     TLFWQKAQKSRLRYAVEKGFAPITVGLILATSLVMSRAADHDWRAYLLTGVCTVIFVF
                     TKTNPLIVVLVAAAIGYLGVV"
     gene            1425..3196
                     /locus_tag="VF874_15295"
                     /pseudo
     CDS             1425..3196
                     /locus_tag="VF874_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897075.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3403..4095)
                     /locus_tag="VF874_15300"
     CDS             complement(3403..4095)
                     /locus_tag="VF874_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177379.1"
                     /translation="MGPVLHFAGTFWWLIFPLSGVIGGGLKAIAAANDRRAERRLERY
                     RIKQQTKIAVAEAAGRARGNEAANRRELTRVLDEHSRTDAKWLEYELDIAKLLDFPMM
                     TDMRNPLTIGFHKAKRRADLLRPETVDDLMGDRDGRLEYRDAVAEYVSAFDIAEAEAI
                     RRRRSDFSSEDQQRIERAQHLMRLATDGGATPQERQSAYSRARKELDGLIALPAMTRA
                     GIERKIAGEIQA"
     gene            4268..4990
                     /locus_tag="VF874_15305"
     CDS             4268..4990
                     /locus_tag="VF874_15305"
                     /inference="COORDINATES: protein motif:HMM:NF036298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5336 domain-containing protein"
                     /protein_id="HEX9177380.1"
                     /translation="MTYSSGPGGPGGYGGPPQPPSSQGFGPQPQGYGAAPAQGKGLPF
                     YLSLGVVVLGILSFFLGFASFAKEDEGYSGDGVFGSSSMSFFDNIGLGVGVIGLAALL
                     AAALIAGFSLLPEQRENGPVVAGLSVTGFISLLFLLIGLSAGANAGIGLILVLITSFL
                     QAALAVAVLLFSAGIIKPPAPKPPQYGYYGPASGGYGPQQQQQPLQQGQPQQPYYGGG
                     GPASGPYQQGPPSQPPQQHNQW"
     gene            complement(4992..>6126)
                     /locus_tag="VF874_15310"
     CDS             complement(4992..>6126)
                     /locus_tag="VF874_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9177381.1"
                     /translation="LVYLRHPTTGGVFASWGSLGHVTIAEPGALIGFLGPRVYEHLYG
                     EPFPPGIQTAENLQRHGVIDGVVPLKWLRRTLERALKVIVDKPATAPEPQVSEPSPDV
                     PAWESVVASRRPDRPGIGFLLRHGATEKVLLSGWGHSPASGRYPHPLAGDGQGHAMLL
                     ALVRFCGQPAVVLGQKRTAGTGPFPRRFALPPGTGPAALREARRGMALAAGLRLPLVL
                     VIDTAGPALSAEAEQEGLAGEIALCLAHLVTLQTPTVSVLLGQGSGGPALAMVPADRV
                     LAALHGWLAPLPPEGASAIVYRNTLHAAELAAAQGIRSTDLLACGIVDAIVPERPDAA
                     DEPIDFSKRLSTAIAGQLAALRQVPAEQRYADRLTRYRRIGLR"
BASE COUNT          919 a         2013 c         2093 g         1101 t
ORIGIN      
        1 tgcacgccaa gctgctgatc gcggacaatc agcacgcctt ggtcggctcg atgaacatcg
       61 accgcagtgc attcgacctg cgtcgagaac tcggcatcac gatcgccgat ccggacgtcg
      121 tctcgcggct caaggaagtc ttcagctcgg actgggacct atcgcatcac tacgacccgc
      181 cggatccgct cgacccgtcc aaacacaccg aagacgattt tccgcacgac cacgacctca
      241 tgcatgagtg agatcgaccc gccgcgcgaa aaggtttcgt tgttcgagct ggcgtggacg
      301 ttcaatcaca tcgccctcgc ttcgttcggt ggcgggctgt cggcgtggtc gcgggaagtg
      361 ctggtggtcg agaagaactg gatgggcgag gaggagtttc tcagcgcgtt gacgatgtgc
      421 cggatcctgc ccggcgccaa ccaggtgaac atggcggtgt tcgtcggcac caagatggcc
      481 ggcatccccg gcgcggtcgt ggcggtgttc ggcctgtgcc tcatgccgct cgtcttcatc
      541 ttgacggtgg catacatcta tttccggttc aaagaggtgc cggcggtgaa gggcagcctg
      601 cacggcgcgt ctgcggcggc cgtggcgctc accctggcca tggtgatcaa gaccggccag
      661 aagtgcttga ccggcctcgt tccggtagtg cttttcgcgg ccagcttcgt cttgaacggt
      721 ctgttgcggt ggcacctgcc ggtgactctg gccattctcg ctccgctaag cctgatctgg
      781 gcatggccga gaaaaacgac cacatgaaca cctacgtaca actggcgatc cttttcacct
      841 cgctgtcctt gctgtcgatc ggcggcggga atgcggtgct gccagagatg cacacgaaag
      901 cagttcgcgg ggagcattgg ctgaccgact cacaattcgc cgacgtgttc tccatttctc
      961 aagccgcgcc cggtccgagc atcctcatcg tcgcgttgat cggatatggc gccgggttgt
     1021 cggtgggagg cgttccggga gcaattctcg gcggagtcat cgcaaccgtc tccatgatca
     1081 caccggccgg gattctcgtt tatgtggtca cgcttttctg gcagaaggcg cagaaatcgc
     1141 ggttacggta tgccgtcgag aaaggcttcg cgccgatcac cgtcggcctg atactggcca
     1201 cctcattggt gatgagccgg gcggccgatc acgactggcg ggcatatctg ctcacgggag
     1261 tttgcacggt gatcttcgtg ttcacgaaga ccaatccact gatcgtggtg ttggtcgccg
     1321 ccgcgatcgg gtatctcggc gtcgtgtgag cagaccgggt tttcgccgcc gcgaaggagg
     1381 aagccgttga cctctaccat tgttgaggtt ttaccgtggg ggcaatgagc atcgaatcgg
     1441 tggccaggac caccctgtac cggcagatga gcgcacgcgg cggcgacctg cgctcgctgg
     1501 ccgaccgggc gttgatcggc cggatctggc gcttcgccgg ccggcatcac cgtaggctgg
     1561 ccggtttcgt cgcggtgagc gtggcgaccg cgctgctggc ggttgcgacc ccgctgctcg
     1621 cgggcaaggt cgtcgaccat cggtgagggg ctgatcctcg acctgcgcac cgcggtgttc
     1681 gaccacgtgc cgaggatgcc gattgcgttc ttcacccgca ctcgcaccgg agcgctggtc
     1741 agccggctcg gcaacgacgt cattggagcg cagcgcgcgt tctccgacac gttctccggt
     1801 gtggtgtcca acgcggtcac gctgacgctg acggtggcgg tgatgatcgg catctcctgg
     1861 caggtcacgt tcctggcgct gctgctggtg ccgctattcg tcatgccgac gcgccggatc
     1921 ggcgcgacga tggcgcggct gagccgcgag agtgcgatcc acaacgccac gatgaatacc
     1981 cagatgatag aacgcttttc ggcgcccggc gcgacgctgg tcaagctgtt cggcagccct
     2041 tcgcgtgaat ctgctgagtt cgcggtgcgg gccggacggg tgcgcgacat cggggtccgt
     2101 tcggcgatgc tgcagtcgac gttcatgaac tcgctgacgc tgatgtcggc gttggcgttg
     2161 gcgctggcgt tggtctacgg tctcggcggc gtgctggcga tcggcagcca tttggaggcc
     2221 ggtgcgatcg tgtcgttggc gctgttgctg acgcggctgt acgcgccgtt gactgcgctg
     2281 gccaacgcgc gcgtcgaaat cgccagtgcg ctagtcagtt tcgagcgcgt gttcgaggtg
     2341 ctcgacctgg tgccgctgat tcgggagaag ccggacgcgg tcgcgctgcc cgacggccca
     2401 gtgccggtcg agttcgacga cgtccggttc agctacccgt cggccgacaa ggtgtcgctg
     2461 gcgtcgctcg aagaggttgc ggtgctcgac gagcggggcg gagacgaagt gctgcacggt
     2521 atttcgttca cggcggagcc ggggtagatg gtggcactgg tcggctcgtc gggggccggc
     2581 aagtcgacga tcgcgtctct gatggcccgg cttcatgacg tcgactccgg tgcggtgcgc
     2641 ttgaatgatc ttgatgtgcg cgacctttcg ttcgcgtcgc tgcgacaccg tcggcatggt
     2701 cacccaggac gggcatctgt tccacgaaac gatccgctcc aacctgcggc tggccgcgcc
     2761 acacgcaacc gacgacgagc tgtgggatgc gctggagcgg gcccggctcg ccgacgtcgt
     2821 cgcggagatg cccgacggtc tcgacacggt agtgggcgag cgcggatacc ggctctccgg
     2881 cgggcagcgg cagcggttga cgatcgcgcg gctgctgttg gggcggtccc gcgtggtggt
     2941 gctcgacgag gcgacggcat cgctggactc ttcctccgaa gcggctgtgc agcaagcgct
     3001 ttcggaggcg ctcgccgggc gcacgtcgat cgtcatcgcg caccggctgt ccactgtgcg
     3061 tgctgctgat gtgatcctcg tcgtcgagga cggccgcatc gtcgaacgcg gcacccatga
     3121 gcagctggtg gcccgcgggg gtcgttacgc cgagctgtac gttacgcagt tcggtcacga
     3181 cattgccgcg gcctagcgcc gcccgcgccg aaatcgaatt ccaggtcgtg atttcccgaa
     3241 aaccacgacc atggcttcac tctcggtgcg gacgttgccc gccgcggcgt ctgcacgcct
     3301 gggttttgcc gaacgtgcgg ccattgcgaa aatctggacg ggaactcgca gtgggttcac
     3361 gctcggtgtg cgaagctggg gcatccgggc tagcgaaacg gcctacgcct ggatctcgcc
     3421 cgcgatcttg cgctcgatgc ccgcccgcgt catcgcgggc aacgcgatca ggccgtcgag
     3481 ttccttgcgc gcgcggctgt atgcggactg gcgttcctgc ggggtggcgc ccccgtcagt
     3541 ggccagccgc atcagatgct gggcacgctc gatcctctgc tgatcctccg acgagaagtc
     3601 gctgcggcgc cggcggatgg cttcggcctc ggcgatgtcg aacgcgctga cgtactcggc
     3661 gacggcgtcg cgatactcga gccgcccgtc ccggtcgccc atcagatcgt cgaccgtctc
     3721 cggccgcaac aggtcggccc gtcgcttggc cttgtggaag ccgatcgtca gcgggttgcg
     3781 catgtccgtc atcatcggga agtcgagcag cttggcgatg tcgagctcgt actcaagcca
     3841 cttggcgtcg gtacggctgt gctcgtcgag gaccctcgtc agctcccgcc gattcgcggc
     3901 ctcgttgccg cgggcccggc cggccgcttc ggcgacggcg atcttggttt gttgcttgat
     3961 ccgatagcgt tcgagccgcc gctcggctct ccggtcgttg gccgctgcga tcgctttgag
     4021 gccgcccccg atcaccccgc tcagcgggaa aatcagccac cagaacgtcc cggcgaagtg
     4081 cagcacaggc cccatgtgtc cagcatgcca ccgggaacgg gtgggtttcg gcgcagccgg
     4141 atgcggggaa tgatggcagg tggcgaccga cgcggcacga tacagtcagt cccgcgcgtg
     4201 cggcgcgccc gatgagcact caatgacggc acgcataccg acgatgccgt ggacaaggag
     4261 cgatgtcatg acgtactcga gcggcccggg aggcccgggc ggttacggcg gccccccgca
     4321 accaccgagc agtcaaggat tcggccccca gccgcagggc tacggcgccg cgccggcaca
     4381 ggggaaaggc ctgccgttct acctcagcct cggtgtcgtc gtcctcggca tcctcagctt
     4441 cttcctgggg ttcgcgtcgt ttgcgaaaga ggacgagggt tacagcggtg acggcgtctt
     4501 cggcagcagc tcgatgagct tcttcgacaa catcggcctg ggtgtgggcg tgatcggctt
     4561 ggcggctctg cttgccgccg ccctcatcgc gggtttctcg ctgcttcccg agcaacggga
     4621 gaacggccca gtggtcgccg gtctgtcggt gaccgggttc atctctctgc tgtttctact
     4681 gatcggtctg tctgcgggcg cgaacgccgg tatcgggctc atcctggtgc tgatcacctc
     4741 gttcctgcag gcggcgctgg ccgtcgccgt tctgctgttc tcggcgggca tcatcaagcc
     4801 gccggcgccc aagccgccgc agtacggcta ctacgggccg gccagcggcg gctacggccc
     4861 gcaacaacag cagcagcctc tacagcaggg ccagccccag cagccgtact acggcggtgg
     4921 cggcccggcg tcgggcccgt accagcaggg gccgccgtcg cagccaccgc agcagcacaa
     4981 ccagtggtga ctcagcgcaa cccgatccgg cgatagcgag tcaaccggtc ggcatagcgt
     5041 tgctcggccg gcacctgcct cagcgcggcc aactggcccg cgatcgccgt cgatagccgc
     5101 ttggagaagt cgatcggctc gtcggcggcg tccgggcgtt cgggcacgat cgcgtcgacg
     5161 atcccgcacg ccagcaggtc ggttgaccga atgccttgcg ctgcagcgag ttccgcagcg
     5221 tgcagggtgt tgcggtagac gatcgcgctt gccccttcgg gcggcagcgg cgccagccag
     5281 ccgtgcaacg cggccagcac ccggtcggcg ggcaccatcg ccagcgccgg gccgccgctg
     5341 ccctggccga gcagcaccga caccgtcggc gtctgcagcg tgaccagatg cgcaaggcac
     5401 agggcgatct cgcccgcgag gccctcctgc tcggcctccg ccgacagcgc tggcccagcg
     5461 gtgtcgatca ccagcacgag cggcaaccgc agccccgccg ccaacgccat cccgcgacgc
     5521 gcctcgcgca gcgctgccgg gccggtccca gggggtagag cgaagcgacg ggggaaagga
     5581 cccgtgcctg cggtgcgctt ctggccgagc acgacggcgg gttgaccgca aaacctgacc
     5641 agcgccagca gcatcgcgtg cccctgaccg tcccccgcaa gcgggtgggg gtacctcccg
     5701 ctcgcggggg agtgccccca gcccgacagc agcacttttt cggtggcgcc gtgccgaagc
     5761 aggaagccga tgccgggccg atcgggacga cgcgacgcga ccaccgactc ccaggccggc
     5821 acatcggggg agggttcgct gacctgcggt tcgggcgcgg tggccggctt gtcgacgatc
     5881 accttcagcg cccgctccag cgttcggcgc agccatttca gcggcaccac gccgtcgatc
     5941 acgccgtgcc gctgaaggtt ctcggccgtc tggatgccgg gcggaaacgg ctcgccgtag
     6001 aggtgctcgt acacccgcgg gccgaggaac ccgattagcg cgccgggctc ggcgatcgtc
     6061 acatggccga gcgaacccca cgacgcgaac acccctccgg tagtcgggtg ccgcaggtac
     6121 accagg
//
LOCUS       DASWBF010000413         9558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1025485,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000413 DASWBF010000000
VERSION     DASWBF010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025485"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VF874_15315"
     CDS             complement(<1..184)
                     /locus_tag="VF874_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177382.1"
                     /translation="MTETLNAAGLSRLQPTGGDPVDSIRWVSADTLDPNNWNPKPIAP
                     GRAAAPRTFAALYRLVA"
     gene            595..1332
                     /locus_tag="VF874_15320"
                     /pseudo
     CDS             595..1332
                     /locus_tag="VF874_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
     gene            complement(1350..2237)
                     /locus_tag="VF874_15325"
     CDS             complement(1350..2237)
                     /locus_tag="VF874_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9177383.1"
                     /translation="MSDVVTGDAVVVEVQIAQLPVRAVGALIDVTAIFILYLIGVLLA
                     AMTFQQFDDALIAAFLIIFMVLALVGYPVIFETATRGRSLGKMAMGLRVVSDDGGPER
                     FRQALFRALASVVEIWFFAGGPAVICSMLSSKGKRIGDVFAGTVVISERGPKLEPPPE
                     MPPSLAWWASSLQLSGLRPETAQLARQFLSRATQLQPQVRDQMAYRITAEVASQVSPP
                     PPPGVPPQFVLAAVLAERHRRELMRLRPTAPSAPPQPVSQPGYAAPVPQPASPPTQPI
                     PPVGAPAVQPEPGGFAPPG"
     gene            2285..3277
                     /locus_tag="VF874_15330"
     CDS             2285..3277
                     /locus_tag="VF874_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX9177384.1"
                     /translation="MDIDAFVLAHRSTWDRLDRLIKRRRRLSGAEIDEMVELYQRVST
                     HLSMVRSASSDSVLVGRLSSLVARARSAVTGARAPLWSEFVRFWTVSFPVVVYRAWRW
                     SLATAVAFFFVAAVIAFWVAGSPEVQAAVGTPHEIDQLVNHDFASYYSERPAGSFALQ
                     VWVNNSWVAAQCIAFSVLLGLPIPKLLFENAANVGVLAGLMFNAGKGDIFLGLLTPHG
                     LLELTAVFVAAAAGMRLGWSVISPGNRPRTQVLAEQGRAVVSVAVGLTVVLLVSGLIE
                     AMVTPSPLPTFMRIAIGVLAEVAFLAYIVHFGRKAVAAGESGDVADAPDVDLVR"
     gene            complement(3347..4669)
                     /locus_tag="VF874_15335"
     CDS             complement(3347..4669)
                     /locus_tag="VF874_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9177385.1"
                     /translation="MVVTGRTALLALLCVLPVALSPWPATLFWFALLAVLGLVAVDVA
                     LAASTRALRFARDGDVASRLGQSADAVLVAANDGRRRFKGQIRDAWAPSARAEPRAHP
                     VDIPPGHKVSLVTRLRPTRRGDQLAAVVTARSIGPLGMAGRQSSSRVPWQVRILPPFL
                     SRKHLPSRLAKLREIDGLLPVLVRGQGTEFDSLREYVIGDDVRSIDWRATARRADVVV
                     RTWRPERDRRVVLVLDTGRTSAGRVGVDPTAGDPRGWPRLDWSMDAALLLAALASRAG
                     DHVDFIAHDRVLRAGVQNASRTEVLAQLVDAMAPLQPALVESDATAMVAAIQRRTRRR
                     ALVVLLTDLNASSLDEGLMPVLPQLSAKHQVIIAAVADPRVGLLATGRSDAAQVYDAA
                     AAERSRNDRRAIASRLRRSGVDVVDAPPEDLAPALADRYLAMKAAGKL"
     gene            complement(4669..5589)
                     /locus_tag="VF874_15340"
     CDS             complement(4669..5589)
                     /locus_tag="VF874_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9177386.1"
                     /translation="MALRSEIAKAVVGQDSVVSGLVIALLCRGHVLLEGVPGVAKTLL
                     VRAMAAALELEFKRVQFTPDLMPGDVTGSLVYDARTAVFEFRPGPVFTNLLLADEINR
                     TPPKTQAALLEAMEERQVSVEGQARPLPDPFIVAATQNPIEYEGTYQLPEAQLDRFLL
                     KLNVPLPPREQEVQILDRHARGFDPRDLSAVQPVATAADLKSGREAVRQVLVADEVLG
                     YIVDIVRATRQSPSLQLGVSPRGATALLATARSWAWLSGRNYITPDDVKAMARPVLRH
                     RIGLRPEAELEGANPDGVLDGILSSVPVPR"
     gene            complement(5640..6788)
                     /locus_tag="VF874_15345"
     CDS             complement(5640..6788)
                     /locus_tag="VF874_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX9177387.1"
                     /translation="MTSLAPQEILTPKVSQQWRTWRWVLLALAVVVGVAAVSTYLTAP
                     RPGGRMDAESTSPDGGHALMTLLRERGVDVVVAKNVADVERAARPDSLLLVAETYFLV
                     DDDLMARLEKVPGDRLLVEPVSNTRKTLAPEIRIGRAATIGGDPNCDLPEALKAGDAQ
                     FGISDTYEAAGDQPLTSCYDGAVVRYRDGGRTVTVVGSADFMMNSGLLKQGNAALAMN
                     LAGARPRLIWYAPQRVEGERSGGSDIFDLIPDNVGWIVWQLCIVVLLAALWRGRRMGP
                     LVAEKLPVVVRASETVEGRGRLYRSHRARDRAADALRTAALQRTLPRLGLAVNAAAPA
                     VVAAVAERCAKRPEVLHHILFGPPPSSDDDLVNLARELDDIERQVAHS"
     gene            complement(6785..7438)
                     /locus_tag="VF874_15350"
     CDS             complement(6785..7438)
                     /locus_tag="VF874_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX9177388.1"
                     /translation="MPTVLIDRDAAHDAAQRELSKPIYPKENFSERFNEWLTDFIYRI
                     MSKGSEVPGGWFTVAVLLTLLAVAIIVAVRIARRTMRTNRKGDFQLFGTAELTAAEHR
                     ATAERFAAEGNWATAIRHRLRAVARQLEESGALNPVPGRTANELARDAAQSFPELRTE
                     LADAAASFNDVTYGERPGTEPAYRHIATLDEHIRSRRFAASRDAESPHGTESWMPLQ"
     gene            complement(7441..8625)
                     /locus_tag="VF874_15355"
     CDS             complement(7441..8625)
                     /locus_tag="VF874_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177389.1"
                     /translation="MVRMTNDDGGVGPGATPPPPSIGYPGYAAPGYPQSGPIPPPGYA
                     PPYAPSPGYPPPGYWPYPGYPPAGPPRALKPGVIPLRPLGLSDILNAAFAYIRVNPRT
                     TLGLTTIVVVATQIIVLILTAVVPLIIYGQADISQVEGDSASGLVAMLLSLLAAGLTS
                     GLSGVVLSGMLTVVVGRAVFGAHITIREAWDRVRGRLLALLGLVLLEGVGAVLLITVG
                     IVVFAVALAISSVLGVLAAIVLVVAGIAAFIYLWTILSFAPVLIVLERLPVFAAISRS
                     FALVRGSFWRVFGIRALGVLVAGLVSSAVSVPFTIGGEVLGITAESVGAALGAAVLTA
                     VGGAIGQIITAPFTAGIVVLLYTDRRIRAEAFDLVLQTGAGSGAGDSADATDHLWLIR
                     QP"
     gene            8674..9138
                     /locus_tag="VF874_15360"
     CDS             8674..9138
                     /locus_tag="VF874_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX9177390.1"
                     /translation="MAELKDRLRADLTAAMKAQDRLRTATLRILISAIRNEEVSGKQS
                     RELTDDEVLKVLARESRKRGEAAEIYTQNGRGELAANEHAEARVIDEYLPTPLTEAEV
                     ADVADTAIAQIAEEIGERPTVKQMGQVMKAATAIAAGKADGARLAAAVKERL"
     gene            9175..9288
                     /locus_tag="VF874_15365"
                     /pseudo
     CDS             9175..9288
                     /locus_tag="VF874_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
BASE COUNT         1607 a         3158 c         3290 g         1503 t
ORIGIN      
        1 ttgcaaccag ccggtagaga gcagcgaacg ttcgaggagc cgcagctctg ccaggtgcaa
       61 tcggtttggg gttccagttg ttcgggtcca gggtgtcggc ggacacccac cgaatgctgt
      121 caacggggtc tcctccggtc ggttgcagtc gcgataggcc ggcggcgttg agggtctcgg
      181 tcatgcggcc gcctcgatca gcccggcgat gtcgtcggtg ctctcgtagg tgtgattccg
      241 tgccattagg tcggctcgca gctagtccga ccaccctgac cgcaggccga atcacgttgc
      301 gtcgcaattc cttagatgtg acacggcgcg ctggaatcat ggcactgtca acttgaggtc
      361 gtgtggacgt tctgctaatc gcataaagcc tcatacgtca cacgcgacct ggtgaatcgc
      421 ggaggcgaac cggcgaaaat cgccctcgga ctgctcaagt tgacagtgcc ctggggcggc
      481 ttgctctgcc cggacaatcg atcaagatca tttgcagtcg gctgagaact attgcagttc
      541 attgcaatcg atactgagaa tttgcagtcc gaattgagac cctacaagcg ttccagcatc
      601 aactggccgg tggatgttcc cgcgctcacg atgatgcacg actgcgcgct gacggccagt
      661 cacgtgatct tcatggacct gccgatcgtg ttcaacctcg acatcgcgat caagggcgaa
      721 gccgacatgc cgtaccggtg ggacgacaac tacggcgccc gccttggcgt gctgcgccgc
      781 gatgatccgt tcggtgagat ccgctggttc gagatcgacc cgtgctacgt cttccatgtc
      841 gctaatgcct atgacgatgg caattcgatt gtgctgcagg cggttcggta tccggagttg
      901 tggcgcaaca acggcggttt cgaggtggac ggagtgatgt ggagctggcg gatcgatctg
      961 gccgacggca cggtcgacga acgccaactc gacgaccgtg gcgtcgagtt cccccgcatc
     1021 gacgaccggc tggctggtct gcgtgccagg tactccatcg cggtgggcga cggcaacctg
     1081 gtccgctacg acctcgaccg cgcgaccgca gaagagcatc gattcgccgg cgaacccgga
     1141 gaagccgtgt acgtgccatc gggcgcgggt gagctgtcgg gctggtacct ggcctacgtg
     1201 tacgacccgg cgcgcaacgg cagcgacctg gtcatcatcg acgcgtcgga cttcaccggc
     1261 gaacccgctg cgcgcgtgcg gcttccgcag cgcgtgccgc acgggttcca cggcaactgg
     1321 atcaccgact agtcgggccg cggtcgaggc taaccgggcg gtgcgaaccc gcccggttcg
     1381 ggttggaccg cgggcgcccc gacgggcggg atgggctgcg taggcgggga agcaggctgc
     1441 ggaactggcg ccgcgtagcc gggttgcgaa acgggctgcg gcggtgcgct cggagccgtt
     1501 gggcgcaacc gcatcagctc gcggcgatgt cgctcggcga gcaccgcggc gagcacgaac
     1561 tgcggcggca cacccggcgg cggtggcggg gacacttgcg atgccacctc agcggtgatc
     1621 cggtatgcca tctgatcgcg cacctggggt tgcaattgcg tagcgcgcga gaggaattga
     1681 cgagccagct gtgcagtctc cggcctcagg ccggacagct gcagcgacga cgcccaccag
     1741 gccagcgatg gaggcatctc cggcggcggc tccagcttcg gcccacgttc gctgatcacc
     1801 accgtgcccg cgaatacgtc gccgattcgt ttgcctttcg acgacagcat gctgcagatc
     1861 accgcgggcc cgccggcaaa aaaccagatc tccaccaccg aggccaacgc acgaaacagc
     1921 gcctgtcgga atcgttccgg gccgccgtcg tcggacacca ctcgcaagcc catcgccatc
     1981 ttgcccagcg accgtccccg ggtggccgtc tcgaagatca ccggatagcc gaccaacgca
     2041 agcaccatga agatgatcaa gaacgcggcg atcagcgcgt cgtcgaactg ctggaaagtc
     2101 atcgcggcca gcagcacccc gatgaggtag aggatgaaga tcgcggtgac gtcgatcagc
     2161 gcaccgacgg cacgcaccgg cagctgcgcg atctggacct cgacgacgac cgcatcaccg
     2221 gtcaccacgt cagacatacc gccgaaggct accgctactc gtttcgtttc atagactcgc
     2281 cgacgtggac atcgacgcgt tcgtcctggc ccaccggtcc acgtgggacc ggctcgatcg
     2341 cctgatcaag cgtcggcgcc ggttgtcggg ggccgagatc gacgagatgg tcgagctcta
     2401 ccagcgggtg tcgacgcact tgtcgatggt gcgttcggcg tcgtccgact cggttctggt
     2461 cggccggttg tccagtctgg tggcgcgggc ccggtcggcg gtgacgggtg cccgcgcgcc
     2521 gttgtggagc gagttcgtcc ggttctggac agtgagcttc ccagtggtgg tgtaccgcgc
     2581 gtggcggtgg tcgctcgcca cggcggtggc gttcttcttc gtcgccgcgg tgatcgcgtt
     2641 ctgggtggcg ggcagtcccg aggtgcaggc cgctgtcggg accccgcacg agatcgacca
     2701 actcgtcaac cacgatttcg cctcctacta cagcgagcgc ccggcgggat cgttcgcgtt
     2761 gcaggtctgg gtcaacaact cctgggtcgc ggcgcagtgc atcgcgttct cggtgttgtt
     2821 gggcctgccg attccaaagt tgctgtttga gaacgccgcc aatgtcggcg tgctcgcggg
     2881 gttgatgttc aacgcgggca agggcgacat cttcctcggc ctgctcaccc cgcacggtct
     2941 gctcgagttg acggcggtgt tcgtggccgc ggccgccggg atgcggctgg gctggtcggt
     3001 gatctcgccc ggcaaccggc cgcgcaccca ggtgctggcc gagcagggcc gggccgtcgt
     3061 ctccgtcgcg gtcggactga ccgtggtgct gctggtgtcg gggctgatcg aagcaatggt
     3121 cacgccgtcg ccgctgccga cgttcatgcg gatcgcgatc ggcgtgctcg ccgaggtcgc
     3181 ctttctcgcc tacatcgtcc acttcgggcg taaggcggtg gccgcgggcg agtccgggga
     3241 cgtcgccgac gccccagacg tcgacctcgt gcggtgagcg aaaccggggt ttcgcacaca
     3301 aagtacgagt agcggcgtgc caaactccgg tttcgccgga ggttttctaa agcttgccgg
     3361 ccgccttcat cgccagatac cggtccgcta gcgccggcgc caagtcctcc ggcggcgcgt
     3421 ccaccacgtc gaccccgctg cgccgcagcc gggacgcgat tgcccgccgg tcgttgcgcg
     3481 accgttcggc cgcggccgcg tcgtacacct gcgcggcgtc cgaccggcct gtcgcgagca
     3541 ggccaacccg cggatcggcg accgcggcga tgatcacctg atgcttggcc gacagttgcg
     3601 gcagcaccgg catcagcccc tcgtccaatg aggatgcgtt gaggtcggtc aacagcacca
     3661 ccaaagcacg gcggcgggtt cggcgctgta tcgcggcgac catcgcggtg gcgtcggatt
     3721 cgaccaacgc gggctgcagc ggcgccatcg cgtcgaccag ctgtgccagc acctcggtgc
     3781 gcgacgcgtt ctgcacgccg gcccgcaaca cgcggtcgtg cgcgatgaag tcgacatggt
     3841 cgccggcccg cgaggccagc gctgccagca gcagcgcggc gtccatcgac cagtccagcc
     3901 gcggccagcc tcgggggtcg ccggcggtgg ggtccacgcc gacgcgtcct gcggaggtgc
     3961 gtccggtgtc gagcacgagc accacccggc ggtcgcgttc gggccgccag gtccgcacca
     4021 ccacgtcggc ccggcgggcg gtggcccgcc agtcgatcga ccggacatcg tccccgatga
     4081 cgtactcgcg cagcgagtcg aactcggtgc cctgcccccg gaccagcacc ggcagcagcc
     4141 cgtcgatttc acgcagcttc gcgagccgcg acggcaggtg cttgcgggac agaaacggtg
     4201 gcaggattcg gacctgccac ggcacccgcg acgagctctg ccgccccgcc atgccgagcg
     4261 ggccgatcga gcgtgcggtc accaccgccg cgagctggtc gccgcggcgc gtcggccgca
     4321 gccgggtgac cagcgacact ttgtgcccag gcgggatgtc gacgggatgc gcacgcggct
     4381 ccgccctcgc gctgggtgcc caggcgtcgc ggatttgacc cttgaaccgg cgccgcccgt
     4441 cgttggctgc taccagcacc gcgtcggcgg actgcccgag gcgtgacgcg acgtccccgt
     4501 cgcgggcaaa ccgcaacgcg cgggtgctcg ccgccagcgc gacgtcgacg gcgaccaacc
     4561 cgagcaccgc cagcagggcg aaccagaaaa gcgttgcagg ccaaggtgat aacgccaccg
     4621 gcaaaacgca cagcagcgcc agcaaggcag tgcggccggt gacgaccact agcgcggcac
     4681 cgggaccgag gacagaatgc cgtccagcac gccgtcgggg ttggctccct cgagctcggc
     4741 ctccggccgc agtccgatgc gatggcgcag caccggtcgg gccatcgcct tgacgtcgtc
     4801 gggagtgatg tagttgcggc ccgatagcca cgcccacgac cgggcagtgg ccagcagcgc
     4861 cgtcgcgccg cgtggggaca ccccgagctg cagcgacggt gactgccgag tggcgcgcac
     4921 gatgtcgacg atgtagccca gcacctcgtc ggccaccaac acctgccgca ccgcctcgcg
     4981 acccgacttc aggtcggcgg ctgtcgcgac cggctgcacg gcggacaggt cgcgcgggtc
     5041 gaagccgcgc gcatgccggt cgaggatttg gacttcctgc tcgcgcggcg gcagcggcac
     5101 gttcagcttg agcaggaagc ggtcgagctg cgcctcgggc agctggtagg tgccctcgta
     5161 ctcgatcggg ttctgggtgg cggcgacgat gaacgggtcc ggcagcggcc gcgcctggcc
     5221 ctcgacgctg acttggcgct cctccattgc ctccagcagc gcggcctgcg tcttgggtgg
     5281 agtgcggttg atttcgtcgg ccagcagcag gttggtgaac accgggccgg ggcggaactc
     5341 gaacacggcc gtgcgcgcgt cgtagaccag cgacccggtc acgtcgccgg gcatcaaatc
     5401 gggcgtgaac tgcacccgct tgaattccag ttccagcgct gcggccatcg cgcgcaccag
     5461 cagcgtcttg gcgacgccgg gcacgccttc gagcagtacg tggccgcggc acagcaacgc
     5521 gatcaccagc ccggagacga cggagtcctg accgacgacg gccttggcga tctcggaccg
     5581 cagcgccatc aatttggcgc gcgcgtcttg tgaggtctgg tcggaaacgg cctgctgtgt
     5641 cacgagtgtg cgacctgcct ttcgatgtcg tcgagttcgc gagcaaggtt gacgaggtcg
     5701 tcgtcggacg acggtggggg tccaaagagg atgtggtgca acacttccgg ccgcttggcg
     5761 cagcgctcgg ccaccgcggc caccaccgca ggcgccgctg cgttgacggc cagccccagt
     5821 cggggcagtg tgcgctgcag cgcggcggtg cgcagcgcgt cggcggcccg gtctcgggca
     5881 cgatgcgacc ggtacaagcg gccgcggcct tcgacggtct ccgacgcacg caccacgacc
     5941 ggcagcttct cggccaccag cggtcccatc cgacggccac gccacagcgc ggcaagcaac
     6001 accacgatgc aaagctgcca cacgatccag ccgacgttgt cgggaatcaa atcgaaaatg
     6061 tcggagccgc cgcttcgctc gccttccacg cgttgcggcg cataccagat cagccgtggc
     6121 cgcgcgccgg caaggttcat cgcgagcgcg gcgttgccct gtttcagcag accggaattc
     6181 atcatgaagt cggcgctgcc gaccacggtc acggttcgac ccccgtcgcg ataccgcacc
     6241 accgcgccgt cgtagcaact ggtcagcggc tgatcaccgg cggcctcgta ggtgtcgctg
     6301 atgccgaatt gcgcgtcgcc ggccttaagt gcctccggca gatcgcaatt cgggtcgccg
     6361 ccgatcgtcg cggcacggcc gattcggatc tcgggggcca gagtcttgcg cgtgttggac
     6421 acaggttcga ccagaagccg atcaccgggc accttttcga ggcgtgccat caggtcgtcg
     6481 tcgaccagga aatacgtttc ggcgacgagc agcagcgagt cgggccgcgc ggcacgttcg
     6541 acgtcggcga cgttcttcgc gacgacgacg tccacgccgc gctcacgcag cagtgtcatc
     6601 agcgcgtgac cgccgtcggg actcgtggat tcggcgtcca tccggccgcc gggccgcggt
     6661 gctgtcagat aggtgctcac cgcggcgacc ccgacgacga cggcgagcgc caacagcacc
     6721 caccgccagg tacgccattg ctgagacacc ttgggcgtga ggatttcctg cggtgcaagg
     6781 ctggtcactg cagcggcatc catgactcgg tgccgtgcgg cgactcggcg tcacgtgatg
     6841 cggcgaaccg ccgggaccgg atgtgctcgt ccagtgtggc gatgtggcgg tatgccggct
     6901 cggttccggg ccgttcgccg tacgtgacgt cgttgaaaga tgctgccgca tcagctaatt
     6961 cggttctcag ctccggaaac gactgagcag cgtcacgcgc cagctcgttg gcggtgcggc
     7021 ccggcacggg gttgagtgca ccgctctctt cgagttgacg ggcgaccgca cggagccgat
     7081 gacgaatggc ggttgcccag ttcccctccg cggcaaagcg ttccgcagtg gctcgatgtt
     7141 ctgcggcggt cagctcggcg gtgccgaaga gctggaagtc gcccttgcgg ttggttcgca
     7201 tggttcgacg cgcgatgcgg acggcgacga tgatcgctac cgcgagaagg gtgagaagca
     7261 ctgctaccgt gaaccatccg ccgggaactt cggaaccttt cgacatgatc cgatagatga
     7321 agtcggtgag ccactcgttg aaccgctcgg agaagttctc tttcggatag atgggtttgg
     7381 acagttcccg ctgtgccgcg tcgtgggcag cgtcacggtc gatgaggacg gtcggcatgg
     7441 tcagggttgc cggatcagcc acaggtggtc ggtggcgtcc gccgaatcgc ctgcccccga
     7501 gccggcgccg gtctgtagga ccaggtcgaa cgcctccgcc cggatccggc gatcggtgta
     7561 gagcagcacg acgatgccgg cggtgaacgg cgcggtgatg atctggccga tcgccccgcc
     7621 gacggccgtc agcaccgcgg cgcccagcgc agcgcccacc gactcggcgg tgatgccgag
     7681 cacctcgcca ccgatggtga acggtaccga caccgcggag gacaccaggc cggcaaccag
     7741 cacgccgagg gctcggatcc cgaacacgcg ccagaacgat ccgcgcacaa gagcgaaaga
     7801 ccggctgatc gcggcgaaca ccggcagccg ctccagcacg atcagcactg gcgcgaagct
     7861 caggatcgtc cacaagtaga tgaaggcggc gatgccggcg accacgagga cgatcgcggc
     7921 gagcacgccg aggacggagc tgatcgcgag tgccaccgcg aagacgacaa tgcccacggt
     7981 tatcagcagc acagcgccaa cgccctcgag caggaccaac ccgagtagcg ctagcagccg
     8041 gccgcgcacg cggtcccagg cctcgcggat ggtgatgtgc gcgccgaaca ccgcccggcc
     8101 gacgacgacc gtcagcatcc cgctgagcac gacgccagag aggccgctgg tgagtccggc
     8161 cgcaagcagg gacaacagca tggcgaccaa gccggaggcg gagtcgccct cgacctgcga
     8221 gatgtctgcc tgcccgtaga tgatcagagg taccaccgcc gtgaggatca gcacgatgat
     8281 ctgcgtggca acgacgacga tcgtcgtgag cccgagcgtg gtccgagggt tgacccggat
     8341 gtacgcgaac gcggcgttca agatgtcgct gaggcccagt ggccgcagcg gaatgacgcc
     8401 gggtttcagc gcgcggggcg gcccggcggg gggatatccg gggtagggcc agtagccagg
     8461 cggcggatag ccgggcgaag gcgcgtaggg cggtgcgtac ccgggcggcg ggatagggcc
     8521 ggactgcggg tagcccggag ctgcgtaccc cggatacccg atggaaggcg gcggtggtgt
     8581 tgccccaggg ccaacacctc cgtcgtcgtt tgtcatgcgg accatcctga ctagcggcat
     8641 ggcgatgcgc aatcacaggt agcgtctgcg gctatggcgg aattgaaaga ccggctacgg
     8701 gcggatctca cggcggcgat gaaggctcag gatcgactgc gtaccgcgac gctgcgaatc
     8761 ctgatctccg cgatacgcaa tgaagaggtt tctggcaagc agtcccggga gttgaccgac
     8821 gacgaggtgc tcaaggtgct ggccagggaa tcgcgcaagc gtggcgaagc agcggagata
     8881 tacacccaga acggccgcgg tgagctcgcc gccaacgagc acgccgaagc gcgggtgatc
     8941 gacgagtacc tgccgacgcc gctcaccgaa gcggaggtgg ccgacgtcgc cgacaccgcg
     9001 atcgcccaga ttgccgagga gatcggcgag cggccgacgg tgaagcagat gggccaggtg
     9061 atgaaggcgg cgactgcgat cgcggcaggt aaggccgacg gggcccggtt ggctgcggcc
     9121 gtcaaggaac ggctgtagcc gtttcgccat ataccggtgg ggtacccgcg tgatataacg
     9181 cggtttggct acaccctgat gaccgaacag agctgcccga aagagcttgt gcgctatgcg
     9241 gtttcggccg agaaggcggg cttcgacttc gaggtgtcca gcgaccacta attcccgtgg
     9301 tcggtgtcag cgaccggaaa gtttgcgacg gtggcccgcc gtccactgag caactcgacg
     9361 atcgccgtcg acaacggccc cttggcgtcg ggcatcggcg aagagcttcg cagccgcagc
     9421 cgctcaccgc ctgtagcggc gggcgcactg acatccgccc gccagccgtg gggatcttcg
     9481 gtaacccaaa ccgcaaccgc tcccccaacg cacgaactcg cacctctcga acacgcaccg
     9541 ttaccagcta actgcgaa
//
LOCUS       DASWBF010000414         5520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_822025,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000414 DASWBF010000000
VERSION     DASWBF010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822025"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..>5520
                     /gene="pks2"
                     /locus_tag="VF874_15370"
     CDS             333..>5520
                     /gene="pks2"
                     /locus_tag="VF874_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolipid-1 biosynthesis
                     phthioceranic/hydroxyphthioceranic acid synthase"
                     /protein_id="HEX9177391.1"
                     /translation="MRAATATPVAVIGMACRLPGGIDSPQRLWEALLRGDDFVDEIPA
                     NRWDADVYYDPEPGVPGRSVTRWGAFLDDVGGFDCDFFGMTEREATAIDPQHRILLEA
                     SWDALEHAGLDPTTLTGSQTGVFVGLTHGDYELLAADTGAAEGPYGFTGTSNSFASGR
                     VSYTLGLRGPAVTVDTACSSGLMAVHQACRSLHDDESDLALAGGVAVTLEPRKSVSGS
                     LQGMLSPTGRCHSFDVAADGFVSGEGCVVLLLKRLPDAVRDGDRILAVMRGTAANQDG
                     RTANIATPSEQAQVAVYRAALAAAGVDASTVGLVEAHGTGTPVGDPIEYASLAAVYGI
                     DGPCALASVKTNFGHLQSTSGPLGLMKAILVLQHGVVPQNLHFTRLPDEIAAIETGLF
                     VPQDTTPWPTNGHHSRRAAVSSYGLSGTNVHVILEQASHQVPGDSATPAPKAQTSLLF
                     PLSATSAEQLRVTAGRLADWVDESDDRTDAWLWDLGYTLARRRAHRPVRTAVAATGVG
                     ELSAALREVADSDIPYQPAVGDGGRGPVWVFSGQGSQWSRMGADLLAAEPVFAATVAA
                     MEPFIAKESGFSVTEAMSAPETVTGIDRVQPTIFAMQVALSETMKSYGVRPGAVIGHS
                     LGEAAAAVVARALSLEDGLRVICRRSRLMSRIAGSGAMASVELPAQQVLSELSIRGLT
                     DVALSVVASPTSTVVGGETQAIRDLVAAWEQKDVMAREVAVDVASHSPQVDPILDELI
                     EALAGLEPMAPEVPYYSATLWDPRDRPAFDGDYWADNLRYTVRFAAAVRAALEDGYRV
                     FGELAPHPLLTHAVEQSANSLDMPIAALAAMRRELEMPFGLRGFVADVHSAGAAVDFS
                     TQYPGGRLVDAPLPTWTHRELMLSREKQDHQAHGASMLAAHPLLGAHVHLREEPERHV
                     WQGEVGTIAHPWLGDHQIHSVAALPGAAYCEMALAAARATLGEQSEVRDIRFEQTLLL
                     DDETPVSAGATVTAPGLLDFVVETHDDGERIRRAGAVLHAREDAHEPTAHDLPGLMAA
                     HSTRLDGAELRKVFDSVGIQYGPAFSGLAAAHTADGDVTTVLAEVALPGAIRSQQAAY
                     ATHPALLDACFQSVVVHPDVQKAAGGAGLLLPVGVRRLRCYQLTRDAHYCLTRVTASR
                     HGECEADIDVLDQSGTVLLTVEGLRLAGAASESEHADRVLNERLLTIEWDKRELPDSS
                     EGDAGSWLLLSTSDAATALATSVADALTVEGAECTIVSWPMAGDQLVGAEGGAATSLR
                     SHLGGGNLAASNGHGSRKAIKGVVVVTAPPEATEDEPAARRGREYVASVVGVARELAE
                     LPGEPPRLYAVTRSAASVLDDDLANLEQTGLRGLMRVIDSEHPHLSATQIDWDDDGNR
                     VQQLALQLLSGSDEDETAWRNGDWYTARLRPSPLRPGERRTTVVEHEHDGMRLEIRTP
                     GDLQTMEFVAFDRVPPGPGEIEVAVTASSVNFADVLVTFGRYPSFEGHLPRLGTDFAG
                     VVTAVGSGVTEHRVGDHVGGMSPKGCWSTFVTCDARLTAALPPGLSDAQAAAVTTAHA
                     TAWHGLHDLARISSADKVLIHSGTGGVGQAAIAIARAAGAEIFATAGSPKRRELLQDM
                     GIEHVYDSRSTEFADQIRRDTDGYGVDVVLNSLPGAAQTAGLDLLSFGGRFVEIGKRD
                     IYGDTRLGLFPFRRNLSFYAVDLGLMSVTHRDQVHELLNKVYELTADGSLPMTESTHY
                     PIQ"
BASE COUNT          887 a         1816 c         1882 g          935 t
ORIGIN      
        1 ggtcatgacc gggtcccccc ggctagcgtg attgtcctgt gccaaccgca caacggtgcg
       61 tcggccgctg gacgtacagc aaggctttcg tactgtagct agaacactct ctggcgaaac
      121 agtgccgcca cgacgatttc gatggcaccc ccgacgagcc gttgggggct gcggctcgta
      181 gtgtttcagt agccgggcgg agatgatctg gtcgctgcgg atggtttccg gggacaaggg
      241 gcgtcaatcc ggccgcgcat gcggggcgaa aactcgttca caacaaaatg cggtaattag
      301 agccaaatat cgagtaatag ggagtacata cattgcgcgc agcaactgcc acacccgttg
      361 ccgttatcgg catggcctgc cgacttcccg ggggaatcga ttccccgcaa cgcctgtggg
      421 aggcgttgct gcgcggcgac gacttcgttg acgagattcc cgccaaccgc tgggacgccg
      481 acgtgtatta cgaccccgag cccggtgtac ccggccggtc ggtaacgcgt tggggcgcat
      541 tcctagacga cgtcggcggt tttgactgcg acttcttcgg catgacggag cgcgaggcga
      601 ccgccatcga cccgcagcac cgcatcttgc tggaggcctc ctgggatgcc ctcgagcacg
      661 ccggcctcga tccgacgacg ctcacagggt cgcagaccgg tgtcttcgtg gggttgacgc
      721 acggcgatta cgaactattg gccgccgata ccggcgccgc cgaggggccc tacggattca
      781 ccggcaccag caacagcttc gcctccggtc gcgtgtccta caccctggga ctgcgcggcc
      841 ccgcggtcac cgtagacacg gcgtgctcgt cgggcctgat ggccgtgcac caagcctgcc
      901 gcagcctgca cgacgacgaa agcgacctcg ctctcgccgg tggcgtcgcc gtgacgctcg
      961 aaccgcgcaa gtccgtttct gggtcgctgc agggcatgct gtcgcccacc ggccgctgtc
     1021 attccttcga cgtcgcggcc gacgggttcg tgtctggcga gggctgtgtg gtgctgttgc
     1081 tgaagcggtt gccggatgcg gtacgggatg gtgatcgaat cctggcggtc atgcgcggaa
     1141 cggcggccaa ccaggacggc cgcaccgcga acatcgcgac gccatcggag caggcgcagg
     1201 tcgccgtgta tcgggctgca ttggccgcgg ccggcgtcga cgcctccact gttggactgg
     1261 tcgaagcgca cgggaccggc accccggtcg gggatccgat cgagtacgca agcctggccg
     1321 ccgtatatgg gatcgacggt ccatgtgcac tggcttcggt gaagaccaac ttcgggcacc
     1381 ttcagtcgac atccggaccg ctgggcctga tgaaggcgat cctcgtcctg cagcatggcg
     1441 tggtcccaca gaacctgcac ttcacccgtc tgcccgacga gattgccgcg atcgagacgg
     1501 ggctcttcgt gccacaggac accacgccct ggcccaccaa cggtcatcat tcacggcgag
     1561 ccgcggtgtc gtcctatgga ctgtcgggca ctaacgtgca cgtcatcctc gaacaagcgt
     1621 cccatcaagt gcccggtgat tccgcaacgc cagcgcccaa agctcaaaca tccctactgt
     1681 ttccactgtc ggccacgtcg gccgagcagc tgcgcgtgac cgccggccgg ctcgcggact
     1741 gggtggacga gtcagacgac cgaaccgacg cctggctctg ggacctcggc tacaccctgg
     1801 cgcgccgacg tgcacaccgg ccggtgcgca ccgcagtggc ggccaccggc gtgggcgagc
     1861 tgagcgcggc cctgcgggag gtcgccgaca gcgatattcc ttatcagccc gccgtcgggg
     1921 atggcggccg cggcccggtg tgggtgttct cagggcaggg ttcgcagtgg tcgaggatgg
     1981 gcgccgatct gctcgcggcc gagccggtgt tcgccgcgac ggttgccgca atggaaccgt
     2041 tcattgccaa ggagtccgga ttctcggtca ccgaggcgat gtcggcaccg gagacggtga
     2101 cgggtatcga ccgggtccag ccgacgattt ttgcgatgca ggttgcgctg tccgagacga
     2161 tgaagtctta tggggtacgg ccgggcgccg ttatcgggca ttcgctgggt gaagcggcgg
     2221 cggccgtcgt ggcgcgtgcg ctgtcgctgg aggacgggct gcgcgtcatc tgccgccgct
     2281 ctcggttgat gtcccgtatc gcgggcagcg gcgcaatggc atcggtggaa cttcctgccc
     2341 aacaagtgct ttcggaactc tctattcgcg gtttgaccga tgtcgccctt tcggtcgtcg
     2401 cctcaccgac gtcgaccgtc gtcggaggcg aaacccaggc gattcgcgat ctggtggcgg
     2461 cgtgggagca gaaggatgtg atggcgcgcg aggtggccgt ggacgtcgcc tcgcattcgc
     2521 cccaggtcga tccgattctc gacgagctga tcgaggccct cgccggcctc gagccgatgg
     2581 cgccggaagt cccgtactac tcggcgaccc tgtgggatcc gcgtgatcgg ccagcgttcg
     2641 acggcgacta ttgggcggac aacctgcggt acacggtccg gttcgcggcg gccgtgcggg
     2701 cggcgctgga ggacgggtac cgggtcttcg gcgagcttgc gccgcatccc ctgctcaccc
     2761 acgccgtcga gcagagcgcg aacagcctcg acatgcccat cgccgcgctg gccgccatgc
     2821 ggcgcgagct ggaaatgccg ttcgggttgc gcggtttcgt cgccgatgtg cacagcgcgg
     2881 gcgctgcggt ggatttctcc acccagtatc caggagggcg cctggtagac gctccactgc
     2941 cgacgtggac ccaccgcgag ctgatgttga gccgcgagaa gcaagaccac caagcgcacg
     3001 gcgcttcgat gctcgctgcg catccattgc tcggggcgca cgtgcacctg cgggaggaac
     3061 cggagcgtca cgtttggcag ggcgaggtcg gcaccatcgc gcacccatgg cttggcgacc
     3121 atcagatcca cagcgtcgct gcgcttcccg gcgcggccta ctgtgagatg gcacttgccg
     3181 cggcgcgcgc gacgctgggt gaacagtccg aagtccgcga catccgcttc gagcagacgc
     3241 tgctgctgga cgacgagacg ccggtatccg ctggggccac cgtcacggcg cccggccttc
     3301 tcgacttcgt ggtcgagact cacgatgacg gcgaacggat ccgccgggcc ggggcagttc
     3361 tccacgcccg cgaagatgcg cacgagccca ctgcgcacga tctccccggg ctgatggcgg
     3421 cccattcaac ccgcttggac ggcgccgagt tgcgaaaggt gttcgacagc gtcgggattc
     3481 agtacggtcc ggcgttctcg ggtctggccg ccgcgcacac cgccgacggc gacgtcacca
     3541 cggtgctcgc cgaggtcgca ctgcccggcg ccatcagatc gcagcaggcg gcgtacgcga
     3601 ctcaccccgc cctgctcgat gcgtgctttc agtccgtggt cgtgcacccc gacgtccaga
     3661 aggccgcagg cggcgcaggt ctgttgttgc ccgtcggtgt tcgacgactg cgttgctatc
     3721 agctgacccg cgacgcccac tattgcctga cccgggtgac ggcgtcgcga cacggcgagt
     3781 gcgaggccga catcgacgtg ctcgaccagt ccggaacagt gctcttgacc gtcgaaggac
     3841 ttcggctcgc cggcgctgcc tcagagagcg agcatgccga tcgggtgctc aatgagcggc
     3901 tgctgaccat cgaatgggac aagcgggaac tgcccgactc ctccgagggc gacgcgggat
     3961 cctggctgct gctcagcacg tccgacgcgg cgactgcgtt ggcgacgtcg gtggccgatg
     4021 cgctgaccgt cgagggcgcg gagtgcacca tcgtctcgtg gccgatggct ggggaccagc
     4081 tggtgggcgc cgaaggcggc gcggccacat cgctgcgttc ccacctgggc ggcggcaatc
     4141 tcgccgcgtc caacggacac gggagccgga aggccataaa gggcgtggtc gtggtgacgg
     4201 cgccaccaga agccacggaa gacgaaccgg ctgcgcgccg cggtcgcgag tacgtggcat
     4261 ccgtggtggg cgtcgcccgt gagttggcgg agcttccggg tgagccaccc aggctctacg
     4321 cggtgaccag aagcgccgcg agcgttctgg atgacgatct ggccaacctg gagcaaaccg
     4381 gactgcgtgg cctgatgcgg gtgatcgatt ccgagcatcc gcacctgagc gccacccaga
     4441 tcgactggga tgacgatggg aaccgagtgc agcagttggc gctgcaactt ctcagtgggt
     4501 ccgacgaaga cgagactgcc tggcgcaatg gcgattggta caccgcacgc ctgcggccca
     4561 gtccgttgcg cccgggcgaa cggcgaacga ccgtcgtcga acacgagcac gacggtatgc
     4621 gcctggagat ccgcactcct ggtgacctgc aaaccatgga attcgttgcg ttcgaccggg
     4681 ttccgccagg gccgggagag atcgaggtcg cggtcaccgc atccagcgtc aacttcgccg
     4741 acgtcctcgt cacgttcggg cgctacccca gctttgaggg gcacctgccg cggttgggca
     4801 ctgatttcgc cggtgtcgtg accgcggtcg gatcgggggt cactgagcat cgtgtcggtg
     4861 accacgtcgg cggtatgtcc ccgaagggct gctggagcac gttcgtcacc tgcgatgccc
     4921 ggctcaccgc cgcccttcct ccgggcctgt cagacgcgca ggcggccgcc gtgaccaccg
     4981 cgcacgcgac cgcgtggcac ggcctgcacg atctggccag gatttcgtcg gccgacaagg
     5041 tgctgatcca ctccgggacc ggcggcgtgg ggcaggcggc catcgcgata gctcgtgccg
     5101 ctggagccga gatcttcgcg acggccggca gcccgaagcg tcgagagctg ttgcaggaca
     5161 tgggtatcga acacgtctac gactctcgta gcaccgagtt cgccgaccag atccgccgtg
     5221 acaccgacgg gtacggcgtc gacgtcgtgc tcaactccct gccgggcgca gcgcaaacgg
     5281 ccggactcga cctgctgtcc ttcggtgggc gcttcgtcga gatcggcaag cgcgacatct
     5341 acggtgacac ccggctggga ctgttcccgt tccggcggaa cctctcgttc tacgccgtcg
     5401 acctcgggct gatgagtgtc acccatcgag accaggttca cgagttgctg aacaaggtgt
     5461 acgaactcac ggcggacggc tcgctgccaa tgacggagag tacgcactac ccgattcagg
//
LOCUS       DASWBF010000415         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_49316, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000415 DASWBF010000000
VERSION     DASWBF010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49316"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1373
                     /locus_tag="VF874_15375"
     CDS             45..1373
                     /locus_tag="VF874_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX9177392.1"
                     /translation="MRCAVTGATGYVGGRLVPQLLDRGHDVRAVARRPSKLADVPWRE
                     DAEVVRGDLGDADSLLAAFDGADVVYYLVHSMGTSRNFAAEEQRTAGNVVTAARRAGV
                     RRLVYLGGLHPSEAKLSPHLRSRAKVGEILLASGIDTLVLQAGVIVGSGSASFEMIRH
                     LTDRLPVMTTPKWVHNKIQPIAVRDVLHYLVEAAAAAVPESRPWDIGGPDVLEYGEMM
                     QTYADVAGLHRRAIVALPFLTPAIASWWVGLVTPIPSGLARPLVESLHCDAVMDNHDI
                     DAVIPPPPGGLTGYRDSVSLALKPIADGAVETSWSDASREAPSEPLPSDPDWAGAVVY
                     TLKRSGWTAATEQELWKLIESTPAQHDWQVKVREPGRLMLLHKNGNVPGDAWLQMRVG
                     TVSGRGSTYHQRMIFYPKGIPGRIYWYAGLRRQRRRFRETFRDVLDRAAR"
     gene            complement(1370..2920)
                     /gene="purF"
                     /locus_tag="VF874_15380"
     CDS             complement(1370..2920)
                     /gene="purF"
                     /locus_tag="VF874_15380"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX9177393.1"
                     /translation="MTGQQTDHSDSEALENSPREECGVFGVWAPGEEVAKLTYYGLYA
                     LQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFEEQTLAAMEGHVAIGHCRYSTTGSTT
                     WENAQPVFRNTAAGTGVALGHNGNLVNTTELTKKARDLGLINTRGNGGGATTDSDILG
                     ALLAHGAADSTLEQAALELLPNVRGAFCLTFMDENTLYAARDPYGVRPLSLGRLDRGW
                     VVASETAALDIVGASFVRDIEPGELLAIDADGVRSSRFANPTPKGCVFEYVYLARPDS
                     TIAGRSVHKTRVDVGRALAREHPIEADLVIGVPESGTPAAVGYAQESGIPYGQGLMKN
                     AYVGRTFIQPSQTIRQLGIRLKLNPLREVIRGKRLIVVDDSIVRGNTQRALVRMLREA
                     GAIEVHVRIASPPVKWPCFYGIDFATPAELIANAVDNEHEMLEAVRHAIGADTLGYIS
                     LHGMIAATEQPATRLCCACFDGTYPIELPSETALGKNVIEHMLANAARSGAGVQADND
                     NVSALRRP"
     gene            complement(3043..3441)
                     /locus_tag="VF874_15385"
     CDS             complement(3043..3441)
                     /locus_tag="VF874_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HEX9177394.1"
                     /translation="MPARRTVDPALTRAAVLAVADWLRDDSLPTPARDELASAVRLTA
                     RTLAASAHGASVELRVPPFVAVQCISGLQHRRGAPPNVVEADARTWLLLASGLLSVPD
                     AAASGAVQLSGPRAGEIADLLPVLRLEGMT"
     gene            complement(3452..4243)
                     /locus_tag="VF874_15390"
     CDS             complement(3452..4243)
                     /locus_tag="VF874_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9177395.1"
                     /translation="MVNTARASSRTFLVTGAASGIGLATAQRLLAEGGTVIGADLAAP
                     RDDFGERFTFVAADVTDEAAVGDVFAAAPDRVDGVVHAAGVAGGGPVHLLDRAEWDRV
                     IRVNLTGTFLVAKAALARMVDQSRVDGERGSIVTLASIEGLEATAGGSAYNASKGGVI
                     LLTKNIAIDYGPSGIRANAICPGFIDTPMTDSIFGMEGMERPSQLLTREHALHRRGQP
                     EEIAAMAAFLVSPDASFVSGQAIAVDGGYTAGRDHDVVQLFGFPD"
     gene            complement(4251..>4692)
                     /locus_tag="VF874_15395"
     CDS             complement(4251..>4692)
                     /locus_tag="VF874_15395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9177396.1"
                     /translation="GLRLGSAAAFVVAAAGAASTAVPRVRSAMADRTPPAAPATWLAL
                     RIPVGTVWSEEAAYRGALATLAADGFGPRTGRLVQAAAFGLSHIADARATGEPMIGTV
                     VATGAAGWVFAWLAERSGSLVAPMLTHLAINEAGAVAALMVQRR"
BASE COUNT          734 a         1591 c         1651 g          716 t
ORIGIN      
        1 ggctccgaat agcatacgaa gggcgccgac acgggaggaa acagatgcgc tgcgcggtga
       61 cgggcgcgac cgggtacgtc ggcggacggc tggtgccaca gctcctcgac agaggccacg
      121 acgtgcgggc ggtcgccagg aggccgagca agctcgccga cgtgccgtgg cgcgaagacg
      181 ccgaggtggt gcgtggtgat ctcggcgacg ccgattccct gctcgctgcg ttcgacggcg
      241 ccgacgtcgt ctactacctc gtgcactcga tgggcacctc gcgcaacttc gcggccgagg
      301 agcaacgcac agccggcaac gtcgtcaccg cggctcgtcg cgcgggtgtt cggcggctgg
      361 tgtatctggg cggactgcac ccgtccgagg cgaagctgtc gccccatctg cggtcgcgcg
      421 ccaaggtggg cgaaatcctg cttgcgtccg gcatcgacac cctggtgctg caggcgggcg
      481 tgatcgtcgg ctccggctcg gcgtcgttcg agatgatccg gcatctgacc gaccgtctgc
      541 cggtgatgac gacaccgaag tgggtgcaca acaagatcca gccgatcgcg gttcgcgacg
      601 tgctgcacta cctggtcgag gctgccgctg ctgccgttcc ggagtcgcgc ccgtgggaca
      661 tcggcggtcc cgacgtgctc gagtacggcg agatgatgca gacctacgcc gacgtcgcgg
      721 ggctgcaccg gcgcgccatc gtcgcgctgc ctttcctcac accggcgatc gccagctggt
      781 gggttggact ggtgacaccg ataccgtcgg gcttggcccg cccgctggtg gaatcgctgc
      841 actgcgatgc ggtgatggac aaccacgaca tcgacgcggt gataccgcca ccgccgggcg
      901 ggctgaccgg ctaccgcgac tcggtctcgt tggcgctgaa gccaattgcc gacggggccg
      961 tcgagacgtc gtggtcggat gcgtcgcgtg aagcgccatc ggagccgttg cccagcgatc
     1021 cggactgggc cggcgcggtc gtctacaccc tcaagcgctc cgggtggacg gcagccaccg
     1081 agcaagaact ctggaagctg atcgaaagca cccccgcaca acatgattgg caggtgaaag
     1141 tccgcgagcc gggtcgcctg atgctactgc acaagaacgg caatgtgccc ggcgacgcct
     1201 ggctgcagat gcgcgtcggc accgtgtccg gccggggcag cacgtatcac caacgaatga
     1261 tcttctaccc caagggaatt cccggccgca tctactggta tgccggatta cggcggcagc
     1321 gcaggagatt tcgggagacg ttccgcgacg tgctggatcg cgcagcgcgt tagggccgcc
     1381 gcaacgccga cacgttgtcg ttgtcggcct gcacccctgc gccgctgcgc gccgcgttgg
     1441 ccagcatgtg ctcaatcacg ttcttgccca acgccgtttc actgggcagc tcgatcgggt
     1501 aggtgccgtc gaagcaggcg cagcacagcc gtgtcgcggg ctgttcggtg gccgcgatca
     1561 tgccgtgcag cgagatgtag cccagtgtgt cggcgccgat cgcatgccgc accgcctcga
     1621 gcatctcgtg ctcgttgtcc accgcgttgg cgatcagctc ggcgggcgtg gcgaagtcga
     1681 tgccgtagaa gcagggccac ttcaccggcg gggaggcgat ccggacgtgc acttcgatgg
     1741 ccccggcctc gcgcagcatc cggaccagcg cgcgctgagt attgccccgc acgatggaat
     1801 cgtcgacgac gatcaaccgc ttgccgcgga tcacctcgcg cagcgggttg agcttgagcc
     1861 ggatgccgag ctggcggatg gtctgcgacg gctgaatgaa cgtgcggccg acgtaggcgt
     1921 tcttcatcag gccctggccg tacgggatgc ccgactcctg cgcgtagccg accgctgccg
     1981 gggtgccgga ctcgggcacc ccgatcacga ggtcggcctc gatgggatgt tcgcgggcca
     2041 gcgcccggcc gacgtcgacg cgggtcttgt gcaccgagcg tcccgctatc gtgctgtcgg
     2101 gccgcgccag gtacacgtac tcgaacacgc agcctttggg tgtggggttg gcgaaccgcg
     2161 acgaccgcac cccgtcggcg tcgatggcca gcagctctcc gggctcgatg tcgcggacga
     2221 acgacgcgcc gacgatgtcc agcgcggcgg tttccgacgc caccacccag ccgcggtcca
     2281 gtcggcccag cgacagcggg cgcacaccgt acgggtcacg cgcggcgtac agcgtgttct
     2341 cgtccatgaa ggtcaggcag aacgcgccgc ggacgttcgg cagcaactcg agggcggcct
     2401 gctccagcgt ggagtcggcc gcgccgtgcg ccaggagcgc acctaggatg tcggagtcgg
     2461 tggtggcgcc gccgccgttg ccgcgggtgt tgatcagccc gaggtcgcgt gcctttttcg
     2521 tcagctcggt ggtgttgacg aggttgccgt tgtggccaag cgccacgccc gtgccggcgg
     2581 cggtgttgcg gaacaccggc tgggcgttct cccaagtggt ggacccggtg gtggagtagc
     2641 ggcagtgacc gatagcgacg tggccctcca tcgcggccag cgtctgctcc tcgaacacct
     2701 ggctgaccag gccgaggtcc ttgaacacca gcacctgcga gccgtccgca acggcgatgc
     2761 ccgccgcttc ctggccgcgg tgttgcaggg cgtaaaggcc gtagtaggtg agcttggcga
     2821 cttcctcgcc cggtgcccac acgccgaaga cgccgcattc ctcgcggggt gagttctcga
     2881 gtgcttcgct gtcgctgtgg tcggtctgct ggccggtcac ggtatggctg ctcccaagag
     2941 gggtcggtga caccccgagt ctacggtcat cacggtcaga tcacggaatc ggaagcgaca
     3001 gtgtcgcgcg ctttctgcaa cacccgccgc gacagagaga tttcaggtca tgccctcaag
     3061 ccgcagcacc ggcagcaggt cagcgatctc gccggcgcgc gggccggaca actgcactgc
     3121 gccggaggca gccgcatcgg gcaccgacaa caaaccgctg gccagtagca gccaggtgcg
     3181 ggcatcggcc tcgacgacgt tgggcggtgc gccacgtcgg tgctgaagtc ccgaaatgca
     3241 ttgcaccgca acgaaaggcg gcactctgag ctcgacactt gccccgtgag ccgacgcggc
     3301 cagcgtgcgc gcggtgagcc ggacagccga cgcgagctcg tcacgtgcgg gtgtgggtag
     3361 cgagtcgtcg cgcaaccagt ccgccacggc gagaacggcg gctcgggtca gcgcggggtc
     3421 gacggtgcgg cgagcgggca ttgccgatcg gtcagtcggg gaagccgaag agttgaacga
     3481 cgtcgtggtc gcggccggcg gtgtagccac cgtcgacggc gatggcctgg ccggagacga
     3541 aggacgcgtc gggcgacacg aggaatgccg ccatcgcggc gatctcctcg ggctgtcccc
     3601 tgcgatgaag ggcatgctcg cgcgtcagca attgagacgg acgctccata ccctccatcc
     3661 cgaagatgct gtcggtcatc ggtgtgtcga tgaagcccgg gcagatcgcg ttggcgcgga
     3721 tgccgctcgg gccgtagtcg atggcgatgt tcttggtgag cagtatcacg ccgcccttgg
     3781 aggcgttgta ggcgctgcca cccgccgtcg cctccaggcc ttcgatgctg gccagcgtca
     3841 cgatcgagcc gcgctcgccg tcgacccgcg actggtcgac catcctggcc agcgcggcct
     3901 tggcgaccag gaaggtgccg gtgaggttga cgcggatcac ccgatcccac tcggcccggt
     3961 cgagcagatg caccgggccg ccgcctgcga cgccggcggc gtggacgacc ccgtcgacgc
     4021 gatcgggcgc cgcagcgaac acatcgccga cggcggcttc gtcggtgacg tcggcagcga
     4081 cgaacgtgaa ccgctcgccg aagtcgtcgc gaggggctgc caggtcggcg ccgattacgg
     4141 tgccgccttc ggccagcagt cgctgggcag tggccagccc aatacccgac gcagccccag
     4201 tcacgaggaa ggtgcgcgaa ctggcgcggg cagtgttcac gatcggaaga ctaacgacgc
     4261 tgcaccatca aggccgcaac ggcgcccgcc tcgttgatcg ccagatgggt cagcatcggc
     4321 gcgacgaggc ttcccgaccg ctcagccagc caggcgaaca cccagcctgc ggcccccgtc
     4381 gccaccacag tgccgatcat cggttcgccc gtcgcacggg catccgcgat gtgcgacaag
     4441 ccgaacgcgg cggcctgcac cagccgcccg gtccgcggtc cgaacccgtc agcggccagc
     4501 gtcgccaatg caccccggta ggcggcctcc tccgaccaga cggtgcccac gggtatccgc
     4561 agcgcgagcc aggtcgccgg tgcggccggc ggggtgcggt cggccatcgc gctgcgcacc
     4621 cgggggaccg cggtgctcgc ggcgccggcc gccgccacga caaacgccgc ggccgagccc
     4681 agccgcagcc cc
//
LOCUS       DASWBF010000416        12109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_433896,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000416 DASWBF010000000
VERSION     DASWBF010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433896"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..882
                     /locus_tag="VF874_15400"
     CDS             205..882
                     /locus_tag="VF874_15400"
                     /inference="COORDINATES: protein motif:HMM:NF037492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5642 family protein"
                     /protein_id="HEX9177397.1"
                     /translation="MVSTGVDLRQFTLLAVTAATACSAPVHTPPPSPLSPTAAQAKQV
                     NPANIRRMRGSFPPGYEISEIEGVASPATYWGLKPVWSAEPAECGVLADPVAGSPQGL
                     SGSGSGGIVYVAVARAPSASGPDPGVVAACRHWSMESGRTTVTVDLLDPPAIEDAATI
                     SMATASRTIVEGGTATDVHATTATAYLGEHVAFVTVVTDPGSGPSELPPDFASTFLTQ
                     TVAALRG"
     gene            910..1584
                     /locus_tag="VF874_15405"
     CDS             910..1584
                     /locus_tag="VF874_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5642 family protein"
                     /protein_id="HEX9177398.1"
                     /translation="MRLPRMLLALAGIVGVALIGGCSTDTATGVSAAPTPPTVDISKV
                     LSVQSKFGPSFTVRTIGKTAIDPKLLSPMQFHQGLTWTPADCAQYAWMLPAGLQGNIA
                     SVTAEGEGNRFIAMAIETSERVGFNPDSADKCGHVTFTAPEMRGAVDVIDAPRIEGAQ
                     TLGKHRQLQTAAASGELYTYVAYLGDYLVVVTANPLLAANQPVPAVNVQRAQQLLIDS
                     VAAVRG"
     gene            complement(1581..2177)
                     /locus_tag="VF874_15410"
     CDS             complement(1581..2177)
                     /locus_tag="VF874_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX9177399.1"
                     /translation="MTYALQGALFEHADRRMLGDGAWVDVCSGWLHDEDCLFDELLAT
                     MPWRAERRPMYDRVVDVPRLLSFHDLETEPAPHPHIAELRDRLNDTYADELGEPFTTA
                     GLCLYRDGNDSVAWHGDRIGRGLKEDTMVAIVSLGATRVLALRPTGGGPSLRLPQAHG
                     DLLVMGGSCQRTWEHSVPKTGKPCGQRISIQFRPRGVR"
     gene            complement(2206..2637)
                     /gene="soxR"
                     /locus_tag="VF874_15415"
     CDS             complement(2206..2637)
                     /gene="soxR"
                     /locus_tag="VF874_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEX9177400.1"
                     /translation="MDDHELTPGELAHRSGVAISALHFYEREGLISSRRTSGNQRRYA
                     RETLRRVAFIRMSQRLGIPLAKVRQALATLPTERVPTSRDWAKLSAGWRQDLDDRILH
                     LQRLRDNLADCIGCGCLSLRSCALSNPGDVLAEQGPGAVRL"
     gene            2665..3873
                     /gene="arcA"
                     /locus_tag="VF874_15420"
     CDS             2665..3873
                     /gene="arcA"
                     /locus_tag="VF874_15420"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEX9177401.1"
                     /translation="MLSVALGVNSEVGTLRAVILHRPGPELQRLTPGNNDALLFDGLP
                     WVSRAQEEHDTFASLLRSRGVEVMLLADLLTEALSSGAARMHGIAAAVDARRLGHPLA
                     QELSEYLRGLDPDALARVLMAGMTFTELPFSASAELSLVRRMHHGADFVIDPLPNLMF
                     TRDSSIWIGPRVAITSLALPARVRETSLTDIIYAHHPRFTGVRRAYESHTAPVEGGDV
                     LLLAAGVIAVGVGERTTPAGAEALARSLFDDDLAHTVLAVPIAQERAQMHLDTVCTMV
                     DVDAVVMYPNVVDTLSAFTITRTPSGVRIADAESFVSAAAKAMGIDRLRVIDTGLDPV
                     TAEREQWDDGNNTLALEPGVVVAYERNVETNSRLEDSGVEVLPIAASELGTGRGGPRC
                     MSCPAARDPL"
     gene            3870..4907
                     /locus_tag="VF874_15425"
     CDS             3870..4907
                     /locus_tag="VF874_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003914530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX9177402.1"
                     /translation="MRLMRLSGVGLLLAALLGVSAPGVAVACACGGVVSPDLGTRTAD
                     EEALVAMDGGTETIVMRLNLQSSADNAALIMPTPSPASVTLTTPSLFGELAALSAPRV
                     ATRRHWKLSLPFLVGASSRSAPPTVLEQVQLGPLEATTLTGGDVSGVQQWLESHGYTM
                     RPEVVTQLEPYLHQGWSFVAMRLTSDVALDGQLAPVKLEFASDRLVYPMRMSAAARNP
                     QRVVVYTLGQHRMQRNDADVAKQHVDIEYAGSIAGRWHDQALAELASGRTFLTKFAVD
                     ISNPSAITSDFEFSAAPNDDPYQQVIYRDEDVDVTPFVLLGGLLLVVAVVAIVAVVLI
                     LLLRRNRIPGR"
     gene            complement(4904..5773)
                     /locus_tag="VF874_15430"
     CDS             complement(4904..5773)
                     /locus_tag="VF874_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177403.1"
                     /translation="MGEVFRASESVASGAISRGRLRWNYRPIFPDVHVPSGDQVSLRT
                     RTVGAWLWSKRKAVITGRAAAALHGALWVNESSPIELLTSNNRPPSGIITRTERIARD
                     EVLERHGMAVATPQRTAFDLGRFLPRDAAVAHLDALARATGITADDLLPLAIRYKGAR
                     GVRRFKTAIDLMDAGAQSPQETRLRLLFIDAGFPRPQTQIPVLDDDGYAFAFLDMGWE
                     DVMVSAEYDGEHHRTDPVQYRKDIRRAENVQRKGWNNIRVIAGDQPADILDRVRTAWA
                     ISESEARAVKRAS"
     gene            complement(5900..7396)
                     /locus_tag="VF874_15435"
     CDS             complement(5900..7396)
                     /locus_tag="VF874_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX9177404.1"
                     /translation="MISPGPHVPVADFGPTDRLQGWAMTAVVAALAGITRFISVSYPT
                     DAGTPIFDEKHYVPQAWQMLSNGGVEDNPGFGLVVHPPMGKMLIAIGEAIFGYNGLGW
                     RFSGAFAGFVLVILVARIARRLSRSTLVGGIAGLLIIADGVSFVTARTALLDGFLTVF
                     VVAAFGALIVDRDQMRERMQVAFAEGRIAETSWGPRLGVRWWRFGAGVLLGMAFATKW
                     SGLYFIVFFAAMSLAFDVAARRQYQVPRPWLGVLRRDTGPTFYAMVIIPFAVYLASYA
                     PWFASETAVNRYEVGRSIGPGGLLPDAVRSLWHYTYGAYNFHAGLTNAAGNHHPWESK
                     PWTWPMSLRPVLYAIDQQNVPGCGEQSCVKAVMLVGTPAMWWIALPVLLWALWRAAVK
                     RDWRYAAALVGYCAGWLPWFADIGRQMYFFYAAPMAPFLVIMIALILGDMLYQPGQSR
                     ERRTLGLLVASFYVALVIANFAWLFPVLTGIPISQATWNMEIWLPSWR"
     gene            7468..8301
                     /gene="rsmI"
                     /locus_tag="VF874_15440"
     CDS             7468..8301
                     /gene="rsmI"
                     /locus_tag="VF874_15440"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX9177405.1"
                     /translation="MGGRLLLGATPLGQPSDASARLVSALGSVDVVAAEDTRRVRTLA
                     SALGVRIPGKVLSLFDQNEAARVPTLVADMKAGATVLAVTDAGMPLVSDPGYRLVTAC
                     IVEGIPVTCLPGPSAVTTALAVSGLPSERFCFEGFAPRKRSARRAWLATLVAEQRTCV
                     FFESPRRLAACLGDAAEELGADRRAVVCRELTKVHEEVVRGSLGELADWAAAGVLGEI
                     TVVLAGAQPRADVVALVAEVQALVDAGMRVKDACAEVVAANPGAPSRRELYDAVLRAR
                     T"
     gene            8427..9167
                     /locus_tag="VF874_15445"
     CDS             8427..9167
                     /locus_tag="VF874_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9177406.1"
                     /translation="MALPALVLVHGGGLAADSWDLTVDEIHRLAPELTVLALDMPERR
                     GKPGDLRELTIADYVDSLFGDIETAGLDDIVIVGHSVAGVMVPGVVTKLGAARVREMV
                     LAAAFVPPEGAAIADTITGPAAPLARRGAKTGRIRETPKWLVRFAYLNGVPRTRRRFM
                     AGKLHAESPRILAENVSRQGMPEEVPRTWILTLRDRALSVSLQRKGIDAIGGVQTLIE
                     MDTCHCMMVSEPERLAEILVERCRLRSR"
     gene            complement(9106..10368)
                     /locus_tag="VF874_15450"
     CDS             complement(9106..10368)
                     /locus_tag="VF874_15450"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX9177407.1"
                     /translation="MRIERLDHLGDAPTMLRAVANAAAERGLGSPAALVGDWFGSGAI
                     VAPSVTAAPVGVDEVFAVPPGSCDTGVVGGGWFGYLSYPDAGGDGLGARIPEAAGGWS
                     DCVLRQDADGQWWYESLSGASLPAWIADALRSPVALRHSEVEWDAADRDAHRAGVLEC
                     LEAIGAGEIYQACVCTQFVGRLDGAPVDFFADAVARTAPARAAFVAGDWGAVASLSPE
                     LFLRRHAETVTSSPIKGTLPRHADPSALRASAKDVAENIMIVDLVRNDLSRVAITGTV
                     TVPELLAVRPAPGVWHLVSTVSARVPVEVPIAALLDATFPPASVTGTPKIRARHLLSQ
                     WEPRRRGVYCGTVGLASPVAGCELNVAIRTVEFDARGAAVFGVGGGITADSNPDNEWD
                     ECLHKAAPIIGIAAGNARRGSRPVVPAR"
     gene            complement(10616..11515)
                     /locus_tag="VF874_15455"
     CDS             complement(10616..11515)
                     /locus_tag="VF874_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX9177408.1"
                     /translation="MTQAPEHAERFTHLRPLLFTIAYEILGSATESDDVLQDSYLRWA
                     QVDLATVRDTKSYLAQLVTRQALNARRAGARRREEYVGPWLPEPLLLDEQDPAADVVL
                     AESVSMAMLVVLETLGPDERAVFVLREVFAFDYDEIASAVGKSVATVRQIAHRARAHV
                     QSRRKRFEPVDPQRSEQITAQFLAAAASGDVDALLEMLAPDVTWTADGGGKVTASRRP
                     IVGAKKVAGAIVGIMRIGQRIPNVRIELATCNSAPAVLVYRGESIDLVITVEVADGKI
                     VNFYAIRNPEKLAGIDVRREISR"
     gene            complement(11512..>12109)
                     /locus_tag="VF874_15460"
     CDS             complement(11512..>12109)
                     /locus_tag="VF874_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9177409.1"
                     /translation="RQLARLGVRVVEGTVTEVREDAVVMSDGAELPSAVTIWTAGFGV
                     PDLAARSGLTTDAVGRLLTDETLTSVDDERVVAAGDAAAPSGQPLRMSCQAAGPLGAQ
                     AADTVLSRIAGEQPAVLSQAFVGQCISVGRHAGTVQISHTDDTPTRFYIGGRAAARIK
                     EFICRGTVWSIAREGRKPGSYYWLRGGKRATREPVPVS"
BASE COUNT         1939 a         4080 c         4102 g         1988 t
ORIGIN      
        1 tagttgatgg tggtctgcgc cccgttgtcg gctgacgcga acccggccgg gacggccagg
       61 gcggcgcccg tcgctattgc cgctgcggcg agggtcgatg ctgcgcgcac agccccgcct
      121 ccttcatccc cgaaccagtg tgagtactca gcaagtacat cgctttttgg cgcccgaacg
      181 ttccggaatt gccaaagaat ctctgtggta tcaacgggcg tggatctgcg ccagttcacc
      241 ctgctggcag tgacggccgc caccgcctgt tccgcgccgg tgcacacccc accgccgagt
      301 ccgttgtcgc ctacggcagc tcaggcgaag caggtcaacc cggccaacat ccggcgcatg
      361 cgcggatcct tcccgcccgg ttacgagatc tccgagatcg agggcgtcgc ctcaccggcg
      421 acgtactggg gactgaaacc cgtttggtct gcggagccgg cggaatgcgg cgtgctcgcg
      481 gacccggtgg ccggcagccc gcaaggactg tccggatccg gtagcggggg cattgtctac
      541 gtggcggtgg cccgcgcccc gtcggcgagc gggcccgacc cgggcgtcgt cgcggcgtgc
      601 cgtcattggt ccatggaatc cggccggacc acagtgaccg tcgacttgct ggatcccccc
      661 gccatcgagg atgccgcgac gatcagcatg gccaccgcca gccggaccat cgtcgagggc
      721 ggtaccgcaa ccgacgttca cgccacgacg gccaccgcct acctcggcga acacgtcgcg
      781 ttcgtcaccg tcgtcaccga cccaggctcc ggcccgtcag agctgccgcc ggatttcgcg
      841 tcgacatttc tcacccaaac cgtcgcggcg ttacgcggct gagtcacctc gattcggtac
      901 attgcgcggg tgcggttgcc gcggatgctg ctcgcgttgg cgggcattgt gggtgtcgcg
      961 ctgattggcg gctgctcaac cgacacggcg accggcgtgt cggccgcacc cacaccccct
     1021 acggttgaca tcagcaaggt cttgtcggtg cagtcgaagt tcggcccgtc gtttaccgtc
     1081 cggactatcg gcaagaccgc gatcgatcca aagttgttgt cgccaatgca gtttcaccag
     1141 ggtctgacgt ggacgccggc cgactgcgcc cagtacgcgt ggatgctgcc tgccggattg
     1201 cagggcaaca tcgcctcggt gaccgctgag ggagagggaa accggttcat cgcgatggcg
     1261 attgaaacgt cggaacgcgt gggcttcaac cccgactccg ccgacaagtg cggccatgtc
     1321 acgttcaccg ctccggaaat gcgtggtgcc gtcgacgtca tcgacgcgcc ccgcatcgag
     1381 ggcgcgcaga ctctgggcaa acaccgtcag cttcagaccg ccgccgcgtc gggtgagctg
     1441 tacacctacg tcgcgtatct gggcgactac ctcgtcgtcg tcaccgccaa tccgttgctc
     1501 gccgcgaatc agccggtgcc agccgtgaat gtgcaacgcg cgcaacaact cttgatcgac
     1561 tcggttgcgg cggttcgcgg ctaacgcacg ccgcgggggc ggaactggat gctgatccgc
     1621 tgcccgcacg gtttgcccgt cttcggtacc gagtgctccc acgtgcgctg gcatgacccg
     1681 cccatcacca gcagatcgcc gtgcgcctgc ggcaggcgca gcgagggtcc gccgcctgtg
     1741 ggtctcagcg ccaggacgcg ggtggcgccg aggctgacga tcgcgaccat cgtgtcttcc
     1801 ttaaggccgc ggccgatcct gtcgccgtgc caggcgacgc tgtcgttgcc atcgcggtac
     1861 aggcagaggc ccgctgtggt gaacggctcg ccaagctcgt cggcgtaggt gtcgttgaga
     1921 cggtcacgca gttcggcgat gtgcgggtga ggggccggct cggtttcgag atcgtggaag
     1981 ctgagcagcc gcggcacgtc gacgacgcgg tcgtacatcg ggcgccgttc cgcgcgccac
     2041 ggcatggtgg ccagcagctc gtcgaacagg cagtcctcgt cgtgcagcca gcccgaacag
     2101 acgtcgaccc acgcgccgtc gccgagcatt cggcggtcgg catgctcgaa gagcgcaccc
     2161 tggagcgcgt acgtcatttc gccagtttat cgcacgcgtg ttcgatcaga gccggacggc
     2221 gcctggcccc tgctcggcga gcacgtcgcc ggggttggac aacgcgcagc tgcgcaggct
     2281 caggcagccg cagccgatgc agtcggcgag attgtcgcga agccgctgca ggtgcaggat
     2341 gcggtcgtcg aggtcctggc gccagcccgc ggacagcttc gcccagtccc ggctcgtcgg
     2401 cacccgctcg gtgggcaagg tggccagggc ttggcggacc ttcgccagcg gaatgcccag
     2461 ccgctgcgac atacggatga acgctactcg gcgcaacgtc tcgcgggcgt agcggcgctg
     2521 attgcccgac gtgcgccggc tgctgatcag cccttcgcgc tcgtagaagt gcagggccga
     2581 gatcgctacg ccgctgcggt gggcgagctc gccgggagtg agctcatggt cgtccatcac
     2641 ctcaaccata gttgaggggt taacgtgcta tccgtggctt tgggtgtgaa ctccgaggtt
     2701 ggaacgctgc gcgcggtgat cctgcaccgg ccgggtccag aactgcagcg gttgacgcca
     2761 ggcaacaacg acgcgctgct gttcgacggg ctgccgtggg tgtcgcgcgc gcaggaggag
     2821 cacgacacgt tcgcctcgct gctgcggtca cgcggtgtcg aggtcatgct gctggccgac
     2881 ctgttgacgg aggcgctgtc gagtggggcc gcccggatgc acgggattgc cgccgctgtc
     2941 gacgcccgac gtctcggaca cccactggca caagaacttt cagagtatct gcgcggcctg
     3001 gatcctgacg cgctggcgcg ggtgctgatg gccgggatga cgttcaccga gctgccgttc
     3061 agcgcgtccg cggaactgtc gctggtgcgt cggatgcatc acggggcgga cttcgtcatc
     3121 gacccgctgc ccaatttgat gttcacgcgg gactcgtcga tatggatcgg cccccgggtg
     3181 gcgattacgt cgttggcgct gccggcgcgg gtgcgggaga cgtcgttgac cgacatcatc
     3241 tacgcgcacc acccgcggtt caccggggtg cggcgcgcct acgagtcgca cacggcgccg
     3301 gtggagggcg gcgatgtcct gctgctggcg gcgggcgtga tcgcggtcgg cgtcggcgaa
     3361 cgcaccacgc cggcgggcgc agaagcgttg gcgcgcagcc tgttcgacga tgacctggcc
     3421 catacggtgc tggccgtacc gatcgcgcag gagcgggcgc agatgcatct ggacaccgtc
     3481 tgcacgatgg tggacgtcga cgcggtggtg atgtatccga acgtcgtcga cacgctgtcc
     3541 gcgttcacga tcacccggac gccttcgggc gttcggatcg ccgatgccga gtcgttcgtc
     3601 tctgccgcgg ccaaggcgat gggcatcgac cggctgcgcg tgatcgacac gggcctggac
     3661 ccggtgacgg ccgaacgcga gcagtgggac gacggcaaca acacactggc gctggaaccg
     3721 ggtgtcgtcg tggcgtatga acgcaatgtg gaaaccaatt cgcgcctgga ggattccggc
     3781 gtcgaagtgc tgccgatcgc agcctccgag ctgggtaccg gccgcggcgg cccacggtgt
     3841 atgtcgtgtc cggcagccag ggacccgcta tgaggttgat gcgcttgtcc ggtgtcggtc
     3901 tgctgctggc ggccctgctc ggggtgagcg cgcctggtgt cgcggtggcc tgcgcgtgcg
     3961 gcggagtggt cagcccagac cttggcacgc ggaccgccga cgaagaggcg ctcgtggcga
     4021 tggacggcgg caccgagacc atcgtgatgc ggctgaatct gcagtcgtcg gccgacaacg
     4081 ccgctctcat tatgccgacg ccttcgccgg cgtccgtcac gctcaccacc ccgtcgctgt
     4141 ttggcgaact ggccgcgctg tcggcgccga gggtcgcgac ccggcggcat tggaagttga
     4201 gcctcccgtt cttggtgggg gcttcgagcc gatcggcgcc gccgacggtg ttggagcagg
     4261 tgcagttggg gccgctggag gcgaccacat tgaccggtgg cgacgtctca ggtgtgcagc
     4321 agtggctgga atcccacggc tacacgatgc gaccggaagt cgttacgcag ctggagcctt
     4381 acttgcacca ggggtggtcg ttcgtcgcga tgcggctcac gagcgacgtc gcgctcgacg
     4441 ggcagctggc gccggtgaag ctggagttcg cctcggaccg gctggtctat ccgatgcgga
     4501 tgtctgcggc ggcgaggaac ccgcagcgcg tcgtggtgta cacgttgggc cagcatcgga
     4561 tgcagcgcaa cgatgccgac gtcgccaagc agcacgtcga catcgagtac gcgggctcga
     4621 tcgccggtcg ctggcatgac caagcgctgg ccgaattagc ttctggccga acatttctca
     4681 ccaagttcgc ggtggacatc agcaatccga gcgcgatcac gtcggacttc gagttcagtg
     4741 cggcacccaa cgacgacccg taccagcaag tgatctaccg agatgaagac gtggacgtca
     4801 caccattcgt gctcctcggt gggttgctgt tggttgtcgc tgtcgtggct atcgtcgctg
     4861 tggtgctcat cctcctcttg cgccgaaacc gaattccagg tcgttagctg gctcgtttca
     4921 cagccctggc ttcactttcg ctgatggccc aagcggttcg aacccggtcg aggatgtccg
     4981 cgggctggtc gccggcgatc actcggatgt tgttccagcc tttgcgttgg acgttttctg
     5041 ctcgacgaat gtctttgcgg tactgcactg gatcagtccg atggtgctcg ccgtcgtact
     5101 ccgcggagac catgacgtct tcccacccca tatcaagaaa ggcgaacgcg tagccgtcgt
     5161 cgtcgagcac cggtatctgc gtctgcggcc gaggaaatcc cgcgtcgatg aacagcagcc
     5221 gcagccgtgt ttcttgcggc gattgggcgc cggcgtccat caggtcgatg gcagtcttga
     5281 accgccgcac accgcgcgcc cctttgtaac gaatagccaa cggcagcaag tcatctgcgg
     5341 tgattccggt ggcgcgggcg agcgcgtcca ggtgtgcgac cgcggcgtct cgggggagga
     5401 atctgccgag gtcgaacgcc gtgcgttgtg gcgtcgcgac tgccatgccg tggcgttcca
     5461 agacctcatc acgagcgatt cgctctgtgc gagtgatgat gccgctcggc ggccggttgt
     5521 tcgatgtgag caattcgatc ggcgaggact cgttgaccca cagcgcgccg tgcagcgccg
     5581 cggcggcacg gcccgtgatc acggccttgc gcttcgacca cagccacgcc ccaacggtcc
     5641 tcgttcgtag tgagacctgg tccccggacg gcacgtgcac gtccggaaag atcggccggt
     5701 agttccatcg caatcgaccg cggctgatgg cccccgaggc gacggactct gatgcgcgaa
     5761 agacctcccc cacgggtgct atcgtcgtcg accccgccgc agcgtcgtcg agaatgaaat
     5821 cagggctgtg gataacacaa agtcacgacc tggaattcgg tttcggcgca aatccgggcc
     5881 ggcgcaaagg ccgccggcgc taccgccagc tgggcagcca gatctccatg ttccacgtcg
     5941 cctgagatat cgggatgccc gtcagcaccg ggaacagcca cgcgaagttg gcgatcacca
     6001 gtgcgacata gaaactcgcg acgagtagcc cgagcgttcg tcgttctcgc gactgtcccg
     6061 gctggtacag catgtcgccg agaatcagcg cgatcatgat caccaggaac ggagccatcg
     6121 gcgcggcgta gaagaagtac atctgcctgc cgatgtcggc gaaccacggc agccaccccg
     6181 cgcaatagcc caccagcgcg gccgcatacc gccagtcgcg cttgaccgcg gcccgccaca
     6241 acgcccacag cagcaccgga agcgcgatcc accacatcgc cggcgtgccg acaagcatca
     6301 ccgccttgac gcacgactgt tcgccgcagc cgggaacgtt ctgttggtcg atcgcgtaga
     6361 ggacgggccg cagcgacatc ggccacgtcc acggcttgct ttcccacggg tggtggttgc
     6421 ccgcggcgtt ggtcagcccg gcgtgaaagt tgtaggcgcc gtaggtgtaa tgccacaggg
     6481 agcggaccgc gtccggcagc agcccgcccg ggccgatcga gcgcccgacc tcgtagcggt
     6541 tgaccgccgt ctcggacgcg aaccacggcg cgtaggacgc caggtacacc gcgaacggaa
     6601 tgatcaccat cgcgtagaac gtcggcccgg tgtcgcggcg cagcacgccc agccatggcc
     6661 gcggcacctg gtactgccgg cgcgccgcca cgtcgaacgc cagcgacatc gcggcgaaga
     6721 acacgatgaa gtacagcccg gaccacttgg tggcgaacgc cattcccagc agcacgcccg
     6781 cgccgaagcg ccaccaccgc acgcccagcc ttggccccca actggtttcg gcgatgcggc
     6841 cctccgcgaa ggcgacctgc atgcgctcgc gcatctggtc gcggtcgacg atcagcgcgc
     6901 cgaacgccgc caccacgaac accgtcaaga agccgtcgag cagggcggtg cgcgcggtca
     6961 cgaagctgac cccgtcggcg atgatcagca atcccgcgat gccgcccacc aacgtcgaac
     7021 ggctcagccg ccgcgcaatc cgcgctacca gtatgacgag cacgaaccct gcgaacgcgc
     7081 cgctgaaccg ccagcccaag ccgttgtaac cgaatatcgc ttcgccgatc gcaatcagca
     7141 tcttgcccat cggcgggtgc acgacgaggc cgaagcccgg gttgtcctcc acgccgccgt
     7201 tggagagcat ctgccacgcc tgcggcacgt agtgcttctc gtcgaagatc ggcgtcccgg
     7261 cgtcggtggg atacgacacg ctgatgaagc gggtgatgcc cgccagggcc gcgacgacgg
     7321 ccgtcatcgc ccacccctgc agccggtcgg tcggcccgaa gtcggccacc ggcacgtgag
     7381 gaccggggct gatcatcggg acgtcgcgct cctgggtgcg cggttcgacg tcggcaaccg
     7441 tcacaacggc gatcgtaggc tgtcgccatg ggtggtcggt tgttgctggg cgcgacgccg
     7501 ctgggccagc cgtccgacgc gtccgcgcgg ctggtgtccg cgctgggctc ggtggacgtc
     7561 gtcgcagcgg aagacacccg ccgcgtgcgc acgttggcca gtgcgctcgg tgtccggatt
     7621 cccggcaagg tgctgagcct gttcgaccag aacgaagcgg cccgggtgcc gacgctggtc
     7681 gccgacatga aggccggtgc caccgtgctg gcggtcaccg acgccggcat gccgttggtc
     7741 agcgatcccg gctaccggct ggtgacggcg tgcatcgttg aaggaatccc cgtgacgtgc
     7801 ctgcccggcc cgtcggcggt gaccacggcg ctggctgtgt ccgggctgcc gtccgagagg
     7861 ttttgcttcg aaggcttcgc gccgcgcaag cggtcggcgc ggcgcgcgtg gcttgcgacg
     7921 ctggtcgccg agcagcgcac gtgcgtgttc ttcgaatcgc cacgccggct cgcggcgtgc
     7981 ctgggcgacg cggccgaaga actcggcgcg gaccggcgag cggtggtgtg tcgcgagctg
     8041 accaaagtgc acgaggaagt ggtacgcggc tcgctcgggg aattggcgga ttgggccgcg
     8101 gctggcgtgc tgggcgagat cacggtcgtg ctggcgggtg cgcaaccgcg ggcggacgtc
     8161 gtggctttgg tcgccgaagt gcaagcgttg gtggatgccg gcatgcgggt gaaggacgcg
     8221 tgcgcggagg tggtcgcggc gaatcccggc gcgccgtcgc ggcgtgagct ttacgatgcg
     8281 gtgttgcgcg ctcggaccta gcccttagga gcctagcgcc gaaatctgca ccctggtcgt
     8341 cctccgccga aaaccacagc cctggtgcag aactcggcgg agattgtgcc ctcgcaaagg
     8401 cagcggttga cgtagagtca gcggctatgg cgctgccggc cctcgttctg gtccacggcg
     8461 gcgggttagc cgccgactcc tgggatctca ccgtcgacga aatccatcgg ctggcaccgg
     8521 agttgaccgt gctcgctctc gacatgccag aacggcgggg caagcccgga gacctgagag
     8581 agctgacgat cgccgactac gtcgactcgc tcttcggcga tatcgagacc gccgggctgg
     8641 acgacatcgt catcgttggt cattcggtgg ctggcgtcat ggtgccagga gttgtcacca
     8701 agctaggagc ggcgcgggtg cgcgagatgg tgctagccgc cgcgttcgtc ccgccggaag
     8761 gcgccgccat cgcggacacg atcactggac cggcggctcc tctcgcgcgt cgcggggcaa
     8821 agacaggccg gattcgcgaa accccaaaat ggttggttag attcgcgtac ctgaacggag
     8881 ttccccggac gcgtcgccgc ttcatggcgg gcaagctgca cgcggagtca ccccgcatcc
     8941 tggcggaaaa cgtctcccgc caaggcatgc cggaagaggt tccgcgcacc tggatcctca
     9001 ccctgcgcga ccgggcactc tcggtcagct tgcagcgcaa gggcatcgac gccatcggcg
     9061 gggtgcagac gctgatcgag atggatacct gtcactgcat gatggtcagc gagccggaac
     9121 gactggccga gatcctcgtc gagcgttgcc ggctgcgatc ccgatgatcg gtgccgcctt
     9181 gtgcaggcac tcgtcccact cgttgtccgg attcgagtcg gcggtgatgc cgccgcccac
     9241 accgaagacg gccgctccgc gcgcgtcgaa ctccaccgtc cggatcgcca cgttgagttc
     9301 acaccccgcg acgggagaag ccaaaccaac tgtgccgcaa tacactccgc ggcgacgcgg
     9361 ctcccattgc gacaaaaggt gccgtgctcg gattttcggt gtgccggtca ccgacgcggg
     9421 cgggaacgtc gcgtccagca gcgcggctat cggcacctcg acgggcaccc gcgccgacac
     9481 cgtcgacacc agatgccaca cccctggggc gggccgcacc gctagaagtt ccggcacggt
     9541 gacggttccg gtgattgcga cccggctcag gtcgttgcgc accaggtcga cgatcatgat
     9601 gttctccgcg acgtccttgg ccgaggcgcg cagcgccgac gggtccgcat gccgcggcag
     9661 tgtgcccttg atcggactcg acgtcaccgt ctcggcgtga cgccgcagaa acagctccgg
     9721 ggacaacgac gccaccgcgc cccagtcgcc ggcgacgaac gcggcacggg ccggtgcggt
     9781 tctggcgacg gcgtcggcga agaagtcgac gggtgcgccg tcgagtctgc cgacgaactg
     9841 cgtgcacacg cacgcctggt aaatctcacc cgccccaatt gcttccaggc actccagcac
     9901 cccggcccgg tgtgcgtcgc ggtcggcggc atcccattcg acttcgctgt gccgcagtgc
     9961 gacaggcgaa cgcagcgcgt cggcgatcca ggccggaaga cttgcgccgg aaaggctttc
    10021 gtaccaccac tgcccgtcgg cgtcttggcg cagcacacag tccgaccaac cgccggccgc
    10081 ctccggaatt cgcgcgccga gcccatcgcc cccggcgtcg ggatacgaca gatacccgaa
    10141 ccacccaccg ccgaccacac cggtatcgca ggagcccggg ggcacggcga atacctcgtc
    10201 gacaccgacc ggcgcggccg tcaccgacgg ggcgacgatg gcaccggagc cgaaccagtc
    10261 gccgacgaga gccgctgggc tgccgaggcc ccgttcggcc gcggcattgg cgacggcacg
    10321 cagcatcgta ggcgcatcgc ccaggtggtc cagccgttcg atccgcacgg agcaagcctg
    10381 acgtgttgcg cgagcagacg caaaagcgcc cggcaccgcc gggaattcac tcttctgggc
    10441 gactgccttt gtgcccaccg ccgagcgaca gcagcgtttc cccaggtcgg agtgcgtaac
    10501 aagaagagag cgctcagcga caaccgatgg ctgcaacgca ggtcagcggg tgtcgctacg
    10561 aagctggcaa ttcgaactgt cccgcgaaag gcgtgccggc tgctaaccgc gcgcgttagc
    10621 ggctgatctc ccggcgaacg tcgatgcccg ccagcttctc ggggttgcgg atcgcgtaga
    10681 agttgacgat cttgccgtcg gccacctcga ccgtgatcac caggtcgatg ctctcgccgc
    10741 ggtacaccag cactgcgggt gcgctgttac aggtcgccag ctcgatccgc acgttcggga
    10801 tgcgttgtcc gatgcgcatg atcccgacga tcgcgccggc caccttcttg gcgccgacta
    10861 tcggccggcg cgaggcggtc accttgcctc cgccgtcagc ggtccatgtc acgtcgggtg
    10921 ccagcatctc gagcaacgca tcgacgtcac cgctggcggc cgcggccagg aactgcgcag
    10981 tgatctgctc gcttcgctgc ggatccaccg gctcgaaccg cttgcgccgg gactgcacgt
    11041 gagcccgcgc tcggtgtgcg atctgacgga cggtcgccac ggatttgcct accgcagaag
    11101 cgatctcgtc gtagtcgaac gcgaatacct cgcgtagcac gaaaaccgca cgttcgtcgg
    11161 gacccagggt ctccagcacc accagcatcg ccatcgacac cgactcggcc agcaccacgt
    11221 cggccgccgg gtcctgctcg tcgagcagca gcggctcggg cagccacggc ccgacgtact
    11281 cctcgcggcg acgggcgccg gcgcgccgtg cattgagcgc ctgccgtgtg accagttgcg
    11341 cgaggtagga cttggtgtcg cggacagtgg ccaggtccac ttgcgcccac cgaaggtagc
    11401 tgtcctgcag cacgtcgtcg gattcggttg cggagccaag gatttcgtag gcgatcgtga
    11461 acagcagcgg gcgcagatgc gtaaaccgct cggcgtgttc gggggcctgg gtcatgaaac
    11521 gggcaccggt tcacgcgtag cgcgcttgcc gccgcgcagc cagtagtagg acccgggctt
    11581 gcggccctcc cgagcgatcg accagacggt gccgcggcag atgaactcct tgatccgcgc
    11641 agcggcccgg ccaccgatgt agaaccgggt cggcgtgtcg tcggtatgag agatctgcac
    11701 ggtgcccgcg tgccggccca cgctgatgca ttgcccgacg aacgcctggc tcagcaccgc
    11761 gggctgctcg ccggcgatgc gtgacagcac cgtgtcggcg gcctgggcgc cgagcggccc
    11821 cgcggcctga cagctcatcc gcagtggctg gcccgacggt gccgcggcgt caccggccgc
    11881 gacgacgcgc tcgtcgtcga cgctggtcag cgtctcgtcc gtgagcaacc tgccaaccgc
    11941 gtcggttgtc agcccgctgc gcgccgccaa gtccgggaca ccgaaccccg cggtccagat
    12001 cgtcaccgcg ctgggcagtt cggcaccgtc ggacatcacc accgcgtctt ctcgcacttc
    12061 ggtgaccgta ccttccacga cccgcacgcc gaggcgggcg agctgacgg
//
LOCUS       DASWBF010000417        13010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_773268,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000417 DASWBF010000000
VERSION     DASWBF010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773268"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..812
                     /locus_tag="VF874_15465"
     CDS             384..812
                     /locus_tag="VF874_15465"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9177410.1"
                     /translation="MSSPDQIVLSVGQQRLLLARARRASGEHRDVLRARIVLAAADGA
                     SNAAIGRTLGVCDGTVRKWRRRFCLHGLDGLADRTRTGRSRRFPATVVAEVKALACEL
                     PADGDVPLAKWSCPDLAIEAAQRGIVESVSASTVRRWAKR"
     gene            complement(949..1890)
                     /locus_tag="VF874_15470"
     CDS             complement(949..1890)
                     /locus_tag="VF874_15470"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX9177411.1"
                     /translation="MLDDCFPTMGTVARVVRDGDGGVDLASVFAEIDRRLSRFDSRSD
                     LSRLNADPRASVPAAPLLRAAVSAALRAASLTGGLVDPTLVGALRRAGYGESRAHTRP
                     PSLHQALGAAPPRRPARPHPAAAWRAVEVDDRAGVIRRPPGLELDLAGSVKGWAADLL
                     ATRLEHHGRCAVDCGGDLRVTAGSGAPWEVRVRHPLTGAIAHTFRVRAGGVATSGIDA
                     RLWQRPDGEFAHHLIDPATGAPVWTGLIAATALAPTALEAEALAKAALLSGPSAGRRL
                     LRAHHGGVLVHDDGTVDPVDPSARPPRSGNPATKPAR"
     gene            complement(1913..2146)
                     /locus_tag="VF874_15475"
     CDS             complement(1913..2146)
                     /locus_tag="VF874_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177412.1"
                     /translation="MISPARLTLVAAAAAGGVGEFVRRRRSARNQPSPPSPPPPPLTT
                     QSSPGQIADLQQTYGADMAPRSSRSPGEAAPDG"
     gene            2754..3901
                     /locus_tag="VF874_15480"
                     /pseudo
     CDS             2754..3901
                     /locus_tag="VF874_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000200.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(4082..5482)
                     /locus_tag="VF874_15485"
     CDS             complement(4082..5482)
                     /locus_tag="VF874_15485"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX9177413.1"
                     /translation="MSRYRVDVPVERLPVLPPLPAELRARLDAALAKPAAQQPTWPAD
                     QAEAMRTVLESVPPVTVPSEVDRLNDLLVEVAQGKAFLLQGGDCAETFAGNTEPHIRT
                     NIRALLQMAVVLTYGASVPVIKVARIAGQYAKPRSSDIDALGLKSFRGDMINAFAPDA
                     AAREHDASRMVRAYAYASAAMNLVRAFTSSGQASLRMVHDWNREFVRTSPAGARYEAV
                     ASEIDRALRFMRACGVEDRNLHTAEIFASHEALVLDYERAMLRLDETEAGEPALYDLS
                     AHYLWIGERTRQLDGAHIAFAEVIANPIGVKIGPTMTPELAVEYVERLDPHNKPGRLT
                     LVSRLGNNKVRDLLPPIIEKVQATGHQVIWQCDPMHGNTHESSTGFKTRHFDRIVDEV
                     QGFFEVHRALGTHPGGIHVEITGENVTECLGGAQDISETDLAGRYETVCDPRLNTQQS
                     LELAFLVAQMLRTDTG"
     gene            6372..7448
                     /locus_tag="VF874_15490"
     CDS             6372..7448
                     /locus_tag="VF874_15490"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX9177414.1"
                     /translation="MGGFSFQHRGGRDLLPDALMWLPAAELIEVPGLTPALTLNAWIT
                     PATDSCAAMRHAESAALALTTSIPVGESEQARLISLRETPSEFEWKLLVRRVLHEIAA
                     GAFDKLVLARQLAVDFDRPVTVAHALASLIDHHRVGAVFGVQHGEQWFVGRTPECLLH
                     LDGGRAYTHGLAGSLPRDRDPVRDAQLSHNLQTDPKLEREHAIVADFVQRTLQRHFRD
                     VRTDTAHPVLKLAEIQHRQTRISAVDPRLAPDLLRVAATLHPTPAVGGFPAAPASRWL
                     AAHEPFNRGWYAAPIGWTGADGGGELAVGIRSAMISGTSAVLYAGCGIVEGSDPDEEY
                     NETCVKMRQMLEVLGISDHEMAAR"
     gene            7445..9187
                     /gene="menD"
                     /locus_tag="VF874_15495"
     CDS             7445..9187
                     /gene="menD"
                     /locus_tag="VF874_15495"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX9177415.1"
                     /translation="MNAATALAVVLADEFARCGVTNIVVAPGARSGPLAQALFIQAAQ
                     SGQRLHTRFDERAAGFLALGLAKRSRRPVVVVCTSGTATANLHPAVLEASHSHLPLVV
                     LTADRPPEMRGTGASQTTDQTKLFGSAVRLYTEVGTPCLERESTPSANSYWRSLVCRA
                     VASAASGPVHLNVPFPEPLDFTPADDADVRFPGRPAGKPWIEIEPAAPPTLSRCTVTV
                     AAAARGVVVVGDDAEDPAAAAAFAEAVGWPLLAEPQSDARTGPNAITTYRYLLAHPAT
                     RARLMPEIVVSVGRPGISREVQALLRDVDEIIVVDPHHDWADPTRSAHRVLRLLPTPE
                     WPAADSSWLRLWRAADQTAQRALDSFLDDSPYSEPRLVRDVLRQLPDDALCVIGSSMP
                     IRDADVTMPQRRGLRVLCNRGLAGIDGTTSTAIGAAIAHQAAGGGNAYAIMGDLTFLH
                     DLTGLVRGPGTAQPDLTIIVINNQGGGIFSLVGHTADALGFDTLFAAPHSVDIASLAA
                     GAGWQHTAVTGPSELTASLSGGGPRIVEIRTDRHVNAKLHQRLSAHIGQALDVEGDRW
                     RSRKLPRNNEARTA"
     gene            9139..10176
                     /locus_tag="VF874_15500"
     CDS             9139..10176
                     /locus_tag="VF874_15500"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9177416.1"
                     /translation="MALTKTAPQQRSEDRVMELDDEAERLYLREKLHEALAPLHLRGL
                     WQLPPGSLPTSPTPVTQACHWRASDVIPLAHQATEVITADGERRAVLLTNPALGPTPF
                     TTTTIQGAVQCLPAHETVAAHRHTPAAIRFILRGAGSYTTVGGVKCEMAPGHFIFTPN
                     WSWHDHTNPTDDPVVWFDGLDIPIVTRLETLIFEDHPDGVQKLTNSTSDMYRTPWEDT
                     DRTLTELFDDPTKIASVQFTDPITGGAISPTLDCWMRRLGPALTTHATRKTGNSIFVV
                     FSGTGETSVGRSRFEWEAGDVFVAPSWAVVDHRPYETSNLFEMTDRPILEALGLFRYE
                     TLEVDALVEVS"
     gene            10173..10754
                     /locus_tag="VF874_15505"
     CDS             10173..10754
                     /locus_tag="VF874_15505"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9177417.1"
                     /translation="MKAIIFDRHGGPEVLTYTNVADPCPGPGEVLICVGALTVNPGPD
                     VMTREGTFGLPGFGLPHIGGSDPAGEVVAVGDGVTTPQVGDRVVVYPVLSCGSCDFCQ
                     PGSGENHCENWRLWGAQTWGGRAELAKVPAANVVSLPESVSWEAAAALPVTYLTAWHG
                     LVDCAGVNHRDTVLVLGAAGGVGVAAIQTRFRE"
     gene            complement(10931..12397)
                     /locus_tag="VF874_15510"
     CDS             complement(10931..12397)
                     /locus_tag="VF874_15510"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX9177418.1"
                     /translation="MSRIRIDKGKGKRRRVRVGRADPSVTGNAGMAAVTELCERLDVI
                     GLLNAATGPIKQRDRGFGPGQLLVGMAAAQLAGQDFLVGLDRHRADVAGQALTPVPGL
                     ASTTAAGLARRMTEQHWRATESGLAAVTERMLALLPAERSAALCEGQATIDMDATDVE
                     VYGRKKDGVAYNHQGQRVGRPHVASWAETETVLAADLGSGVDDPRATAEDLLNRALAG
                     LPRRIRRERVTLRADAGYFAGQLARAAHTAGIGFAIGAKRVTTMWRALAGIAETDWRA
                     AIDMPDAQVAVSDYAPADWPPDTRLLIRRVRLWPDQVSADPRSRRRGTLHPDQRALPL
                     AELADADAIYAYSFILTSLDVSTPDQAAAVEHWYRHRTSVENIFRDSKHGAALRHLPS
                     GNKQVNTAWMWGALLAASIAGWLHQLTAVLADDDTDIVAGHGVHGGKAMIATLRHRLI
                     AIPARVIRHARTLTLRPEPGCRDLLDLILTRLRALPAP"
     gene            12642..>13010
                     /locus_tag="VF874_15515"
     CDS             12642..>13010
                     /locus_tag="VF874_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9177419.1"
                     /translation="MSLRFAFYGRVSTEDAQDPEASRSWQKRRAIDLITPHGGVLAAD
                     YFDVGQSRSLPWKRRPEASRLLADVTASDRSFDAVVIGEPARAFYGPQFALTFPVLTH
                     YGVGLWVPEVGGAVDPGSEAH"
BASE COUNT         2078 a         4366 c         4403 g         2163 t
ORIGIN      
        1 cgatcacatg tccccctcat tcagctgagt agcgatccgc tcgtcgaccc tacgccgggt
       61 ggttaggaca cgtccgtggc ggtgactttg ttgttacgtg ggcaggctcc accgcaggta
      121 caagcgcgct gaagtcacga ctccggcatg cgcatcacct gctcagcgac gcgatccgca
      181 gttctgctcg tagtcgtccc cgggcttgct aatctgcaat ttcgtctgca acatccgccg
      241 cgccggcgcg catgatcagc cggtaacgca gtgaattctt cgtcgactcc gggggtgtgg
      301 gcacggcatc catgatgctc gaaccgatgc gcggcgtgtc gccaacgggg ggccggacaa
      361 cgggccacaa acggtctatg tctatgtcga gccctgatca gatcgtcctt agcgttgggc
      421 agcagcggct gctgttggcc cgggctcgcc gagccagcgg tgagcatcgc gatgtgctgc
      481 gcgcccggat cgtgttggcc gctgcagacg gcgcgtccaa cgccgcgatc ggcaggaccc
      541 tgggtgtttg tgacggcacc gtccgaaagt ggcggcgccg gttctgcctg catggcctgg
      601 acggtcttgc tgatcgcacc cgcaccggtc ggtcgcgccg cttcccggcc acggtggtgg
      661 ccgaagtgaa ggcgctagct tgcgagttgc ccgccgatgg cgatgtgccg cttgccaagt
      721 ggagctgtcc ggacctggct atcgaggccg cgcagcgcgg catcgtcgag tcggtgtcgg
      781 cctccacggt gcgtcgctgg gctaagcgct gacgcgctca agccgtggca gcatcgatcg
      841 tggatctttc cgcgtgaccc ggacttttgc cgtcaaagca gcccgcgttc ttgacctcta
      901 cgcgcggatc tgggacggtg atacgtactc agcggtcggt caggagcgtc atcgagccgg
      961 cttcgttgcg gggttccctg atcgaggcgg tcgagcggag ggatccaccg gatcgacggt
     1021 gccgtcgtcg tggacgagca cgccgccgtg gtgggcccgt agcagccggc gtcccgcact
     1081 ggggcccgac aacagagcgg ccttggccag cgcctcggcc tcgagtgcgg tcggcgcgag
     1141 cgcggtggct gcaatcaacc cggtccagac aggggctcca gtagcggggt cgatcaggtg
     1201 gtgggcgaat tcaccgtccg gacgctgcca cagtcgcgca tcgatgccag atgtcgccac
     1261 cccgccggcg cggacgcgga acgtgtgcgc gatcgcgccg gtcaacggat gacggacgcg
     1321 cacctcccac ggcgccccgc tcccggcggt cacacgcagg tcgccgccac agtcgacggc
     1381 acagcgccca tgatgctcga gccgcgtggc caggaggtcg gccgcccatc ccttcaccga
     1441 ccctgcgagg tccagctcga ggccgggtgg ccggcgaatc acgcccgcac gatcgtcgac
     1501 ctcaaccgcg cgccacgcgg cggccgggtg gggccgggcc gggcgccgtg gcggcgctgc
     1561 gccgagcgct tggtgcagcg acggaggtcg ggtgtgcgcg cgggactcac cgtagcccgc
     1621 gcgacggagc gccccgacca gcgtcgggtc gacaaggccg ccggtcaggg acgccgcgcg
     1681 caacgcggcc gacaccgccg cgcgcagcaa cggcgccgcg ggaacactcg cccgcgggtc
     1741 cgcgttcagc cgtgacagat cgctgcggga gtcaaagcgc gacagccgtc ggtcgatctc
     1801 ggcgaacacc gatgccaggt cgaccccgcc gtcgccatcg cgcacgaccc gggcgacggt
     1861 gcccatcgtc gggaagcagt cgtcgagcac gcgtctcctc gcggccgcgc ggtcacccgt
     1921 ccggcgcggc ctcgcccggg gagcgcgagg agcgcggagc catgtccgcg ccgtaggtct
     1981 gctgcaggtc cgcgatctgc cccggactcg actgtgtcgt cagcggcggc ggcggcggcg
     2041 acggcggcga cggctggttc cgcgccgacc gccggcggcg gacaaactcg cccacaccgc
     2101 cggccgcggc ggcggcaacg agcgtcaggc gcgccggcga gatcatggac accagcctaa
     2161 ggcccgcccg ttcccccggc gagcgtgacg ggccgaattt tccctgcggc gaagtaccca
     2221 ggtgggaaac tacgtacggg gctcggcatc tgccacggtt atcgacggtc gccgcagcgc
     2281 caaggcggat cgtgcacggt gccaaggcgc accggcagcg gcagcttgtg gcgatcggct
     2341 gacccggtta cgtgtccgcg agagtccaca gaacgctagc ggcacacctc agaagacgag
     2401 agtttggacg tgctcatgct gacctgcttc gtcctcccac tgaatacgga ggttggcacg
     2461 atccgcagtg cccgagtgca gcaacaatga gaactcgacg cacgttgagc tgatcacgca
     2521 cggtcgccag gaggtgtcga cgggcatgct cgaccagttc gtcaacaccc gagtattcgc
     2581 ggtagagacc ccaggactta gctcgcgcct tgaatgcctg gaccgcctcg aattgctccg
     2641 tgtcgattga tccgggaacg acaggtcgtg taggaaagtt ctgtatttac tcgcctcccc
     2701 gcgatgcgca cgccgacaac ggatttcata cgtcaacggg agaggtggtc agggtggaag
     2761 tagtgcatcg gcggtgtgcg ggtatcgaca tttcgaagaa ggacgcgaag gtgtgcgtcc
     2821 gggttcaggg ctccgggcga cgagcgacaa cggcgaccgt gacgacctgg ggtgcgatga
     2881 gcaacgagat cctggcgttg cgtgagcacc tggtcaccga gaaggtcacg tgcgtggtga
     2941 tggagtcgac gggcgactac tggaagccgt tctactacct actcgaagac gggttgaacg
     3001 tgatgctggt caacccgcgc gacgcccgca accggccggg ccggtaaacg gacgtctccg
     3061 atgctgcgtg gctcgccgac ctcggcgcgc acgggctgtt gcgggcctcg ctggtcccgc
     3121 cgccaccgat ccggcggtta cgtgatctga cccgggcgcg caccgatctg aaccgggacc
     3181 gcactcgcca gatccaccgc ctggagaaga tcctgcagga cgccgggatc aaactgtcct
     3241 cggtcgccac cgacatcatg gggcagtcga gccgggcgat gctggacgcg ctgatcgagg
     3301 gcaaggccga tccggcggca atggccgatc tggcgaaacg ccggatgcgg tcgaagatcc
     3361 cggccctgac cgaggcgttg acggggcggt tcacgcccgt catcggcggc gagctgacag
     3421 cggcggaacg cctcgccgag atcaccggcc tcggccaggt gtccgcgcag gtcatcatcg
     3481 ccgagatcgg actggacatg atgcggtttc ccacggccgg tcatctggtg tcgtgggcga
     3541 atctgtgccc gcgcaccatc cagtccgggc cggtgcagcg tggcgggaag accggcaagg
     3601 ggaatccgta tctcaaggga gtgctgggtg aggcggccgc tgtggctgca ggacggacac
     3661 tttcctcgga gagcgctatc ggcgcatcgt caagcgccgg ggcaagctca aagcgctcgt
     3721 cgctgtcgcc cgctcgatcc tggtcgtcgt ctggaatctg ctggccgatc cgaccgcgcg
     3781 cttccatgat ctcggcagcg attaccacac tcgccgcatc gacatcgact gccgacttcg
     3841 caaccacctc gcccaacttg cggcgctcgg ctaccgcgtc actctcgaac cagccgcctg
     3901 accgaactga cccaacccaa cgtctgacgg aagtcgttct ctgcaaccgc agatccagca
     3961 cgtaaagcag tcagtggtgc gctgcttcac ccgttctcga cataagccag gacagcaaac
     4021 gccgtcagcg cgcgtcaatg cgccgagtgc tgggacgatg caggacgcat tctttgctcc
     4081 ttcatccggt gtcggtgcgt agcatctggg cgacgaggaa cgccagttcc agtgactgct
     4141 gggtgttcag tcttgggtca cagaccgttt cgtagcggcc ggccagatcg gtttccgaaa
     4201 tgtcctgcgc cccacctaga cactcggtga cgttttcgcc ggtgatctcg acgtggatgc
     4261 cgccgggatg ggtgcctagc gcgcggtgca cctcgaagaa gccctgcacc tcgtcgacga
     4321 tccggtcgaa gtggcgggtc ttgaacccgg tggacgactc gtgggtgttg ccgtgcatcg
     4381 ggtcgcactg ccagatcacc tgatgaccgg tggcctgcac cttctcgatg atcggcggca
     4441 gcaggtcgcg aaccttgttg ttgcccagtc ggctgaccag cgtgaggcgt cccggcttgt
     4501 tgtgcgggtc cagccgctcg acatactcca ccgccagctc cggcgtcatc gtcggcccga
     4561 tcttcacacc gatcgggttc gcgatcacct ccgcgaacgc gatatgagcg ccgtcgagct
     4621 gacgtgtacg ctccccgatc cacaggtaat gcgccgacag gtcgtacaac gctggctcgc
     4681 ccgcctcggt ctcatcaagg cgcagcatcg cccgctcata gtcgagcacc agcgcctcgt
     4741 ggctggcaaa gatctcggcg gtatgcaaat tgcggtcctc gacgccgcac gcgcgcatga
     4801 agcgcagtgc acggtcgatc tcgctggcca ccgcctcata gcgggcgccc gccggtgagg
     4861 tccggacgaa ctcgcggttc cagtcgtgca ccatacgcag cgacgcctgg cccgatgacg
     4921 tgaacgccct aactaggttc atagctgcgc tggcgtacgc gtaggcgcgc accatccgcg
     4981 acgcgtcgtg ttcgcgcgcg gccgcgtccg gggcgaacgc gttgatcatg tcgccgcgga
     5041 aggacttcag ccccagcgcg tcgatgtccg acgaccgcgg tttggcgtac tggcccgcga
     5101 tgcgggccac cttgatcacc ggcacgctgg ccccgtaggt cagcaccacc gccatctgca
     5161 gcagcgcgcg gatgttggtg cggatgtgcg gctcggtgtt gccggcgaag gtctcggcgc
     5221 agtcgccgcc ctgcagcaga aacgccttgc cctgggcgac ctcgaccaac aggtcgttga
     5281 gacggtcgac ctccgacggc accgtcaccg gtggcacgct ttccagtacg gtgcgcatcg
     5341 cttcggcctg atcggccggc caggtgggct gctgggcggc cggtttggcc aacgcggcgt
     5401 ccagccgcgc gcgcagttcc gcaggcagcg gcggcagtac aggcagcctt tcgactggga
     5461 cgtcgacgcg gtatctcgac atcatggcca tggccaacgg caaggtgccg ctcgcacccg
     5521 catggtccgg cgcgaccgca ctcccgttgt gttcgggcgc agcagttggc gactcagtgg
     5581 cagctgacat ggcagacctc ccagtgtgga tttttggccg atatcgaaat ctgttgcagc
     5641 actagtccat cgcgtgcttc tggttgaccc agcgctcgag ccgggtctgc aggtcgtaaa
     5701 accacacacc ggcgaacggt gtgcccactg cgagacagac ccccatcaat gtgctcatct
     5761 cgacctcctc atgccagttc cgccgaggct ctctgacaaa cccatgctcg cggcgcgcga
     5821 gaaggtcgat agggtaatcg actactccac tgccgggaca aatacgaatc ggttactcag
     5881 cccgaccgct tgggcaatgg ctccgtcagt tgacagctgt cccgcgcggt acttgcgacc
     5941 tgggtaccta cgcggggagc tcccagatat tcgagtagtc aactacgctg tcgacccggt
     6001 cggccgaggc agacggtggt gaggtgaccg atcaactacg gacgagcctc ccgtcgcagg
     6061 agctggataa cttcgcggcg ttgtgccggc acggtgcgac aactgcggct gatcgggggc
     6121 gaccggtact ggtgagttgg accgccaccg ttccacaact cactgtcgcc aaccaattcc
     6181 ggcgcctttc caccgacagt gggcgcacat ttgtgtgggc gtcgtcgtgg accgggcaac
     6241 gtcggcttgc cgtgggcagc gcacttgacg tgaccggcca tggggttgac aggttcgagc
     6301 aggtgtcttc tgcctggagt cgacaccgcg atggcgcact catcggcggg accggtcggc
     6361 agccttctct ggtgggcggc ttcagcttcc aacatcgcgg cggtcgcgat cttctgcccg
     6421 acgccctgat gtggttgccg gccgctgaat tgatcgaagt cccgggcttg acgcccgcac
     6481 tgacactgaa cgcctggatc acgcccgcga cggacagctg cgctgccatg cggcacgccg
     6541 agagtgccgc cctcgccctc accaccagca ttccggtcgg cgagtcagaa caggcccggt
     6601 tgatctcgct gcgggagacg ccatcggagt tcgaatggaa gctgctcgtc cgccgcgtcc
     6661 ttcacgagat cgcggcgggc gccttcgaca agctggttct ggctcgtcag ctcgccgtgg
     6721 atttcgacag gcccgtcacc gttgcgcatg cgctcgcgtc gttgatcgac caccaccgag
     6781 tcggggcggt gttcggtgtc caacatgggg agcagtggtt tgtcgggcgg accccggaat
     6841 gccttctgca cctggatggc gggcgtgcct acacccacgg attggccgga tcgcttccgc
     6901 gcgaccggga cccagtccgc gacgcgcaat tgagccacaa cctgcaaact gaccccaagc
     6961 tcgagcgcga gcatgcgatc gtcgccgact tcgtccagcg gacgctgcaa cggcacttcc
     7021 gcgatgtccg aaccgatacc gcgcaccctg tcctcaaact cgccgagatt cagcaccgac
     7081 aaacaaggat cagcgcagtc gaccccaggc tggcgccaga cctgctgcgg gtggcggcaa
     7141 ccctgcaccc gacgcccgcg gtcggcggct tcccggcggc gccggcgtcg cggtggctgg
     7201 ccgcacatga accgttcaac cgaggctggt atgccgcacc gatcggctgg accggcgctg
     7261 acggcggcgg cgagctggcg gttggcatcc gctcggcgat gatcagcggc acatcggctg
     7321 tgttgtatgc cgggtgcggc atcgtcgagg gctccgatcc ggacgaggag tacaacgaga
     7381 cctgcgtgaa gatgcgtcaa atgctcgagg tcctggggat ctccgaccac gagatggcag
     7441 cccgatgaac gcggcgacgg ccctggcagt ggtcctggct gacgaatttg cccggtgcgg
     7501 cgtcaccaac atcgtcgtcg cgccgggcgc tcggtccggc ccgctggccc aggcactctt
     7561 tatccaggcc gcacagtcgg gccagcgcct gcacacccgc ttcgacgagc gggcagccgg
     7621 tttcctcgcg ctcggattgg ccaaacgcag tcgacgtccc gtggtcgtcg tctgcacgtc
     7681 ggggaccgca accgccaatc tgcacccggc cgtgctcgaa gcgagtcact cccatctgcc
     7741 gctagtagtc ctgaccgcgg accggccacc ggagatgcgc ggcacggggg cgagccaaac
     7801 cacagaccag acaaagcttt tcggctcagc cgtcagactg tacaccgagg tcggcactcc
     7861 ttgcctcgag cgagagagca ctccatcggc caacagctac tggcgctcgc tggtgtgccg
     7921 cgccgtcgcg tccgcggcga gtggacccgt ccatctgaac gtgccgtttc ctgaaccgct
     7981 cgatttcact cccgcagacg acgcggacgt tcgctttccg ggcaggcctg cgggcaaacc
     8041 gtggatcgag attgagcccg ccgccccgcc gacgttgtcg cggtgcacgg tcaccgtagc
     8101 ggcggcagcg cggggcgtcg tcgtggtcgg tgatgacgcc gaagatcccg ccgctgcggc
     8161 cgcgttcgcc gaggcggtgg gttggccgct gctggccgag ccgcagagcg acgcccgcac
     8221 cggacccaac gccatcacca cctaccggta tctactagcc caccccgcca cccgtgcgcg
     8281 gctcatgccg gaaatcgtgg tcagcgtcgg ccgacccggg atatcgcgcg aagtccaggc
     8341 attgctgcgc gacgtcgacg agatcattgt cgttgatccg caccatgatt gggccgaccc
     8401 cacccgctcg gcgcaccgtg tcctccggct tctcccaaca ccggaatggc ctgccgccga
     8461 ttcgtcctgg ttgcgactgt ggcgggccgc cgaccagacc gcacagaggg cactcgacag
     8521 cttcctcgac gactccccgt acagcgagcc tcgactcgtc cgggatgttc tgcgccagct
     8581 tcccgacgac gccttgtgcg tcatcgggtc gagcatgccg atccgcgacg cggacgtgac
     8641 catgccgcag cggcgaggcc tgcgcgtcct ctgcaatcgc gggctggcgg ggatcgacgg
     8701 cacgacatcc accgcgatcg gcgccgcgat tgcgcaccaa gcggccggcg gcgggaatgc
     8761 ctacgcaatc atgggcgatc tgacgttcct gcacgacctc accggcctcg tgcgtggtcc
     8821 gggcaccgcg cagccggacc tcaccatcat cgtcatcaat aaccagggcg gcggcatctt
     8881 ctcgctcgtg ggacataccg ccgacgcgct cgggttcgac acactgttcg ccgcgccgca
     8941 cagcgtcgac atcgcgagcc tggccgcggg cgcgggctgg cagcataccg cggtcaccgg
     9001 tccgtccgag ctgaccgcca gtcttagcgg tggtggcccg cggatcgttg agattcgtac
     9061 cgaccgacac gtcaacgcca aactgcatca acgactttca gctcacatag gccaagccct
     9121 cgacgttgag ggtgaccgat ggcgctcacg aaaactgccc cgcaacaacg aagcgaggac
     9181 cgcgtgatgg aactcgacga cgaagccgag cggctgtatc ttcgcgaaaa gcttcacgaa
     9241 gcgttggcgc cgctgcacct tcgtgggctc tggcagctgc cgcccggctc cctgccgacg
     9301 tccccgacgc cggtcacgca ggcgtgccat tggcgggcga gcgacgtgat cccgctggcg
     9361 caccaagcca cggaggtgat caccgccgac ggggagcggc gggcggtgct gctgaccaat
     9421 ccggcgcttg gtccgacacc attcaccacc acaacaattc aaggcgcagt gcagtgcttg
     9481 ccggctcacg agaccgtggc cgcacatcga cacacgccgg cggcgatcag gttcatcctc
     9541 aggggggccg gctcctacac cacggtcggc ggggtcaagt gcgagatggc gcccgggcac
     9601 ttcatcttca ccccgaactg gagttggcac gaccacacga atccgaccga cgatcccgtg
     9661 gtgtggttcg acggcctcga cattcccatc gtcactcgtc tcgagacgct tatcttcgag
     9721 gaccatccgg atggcgtaca gaagctgact aatagcacgt cggacatgta ccgcacccca
     9781 tgggaggata ccgaccgcac gttgacggag ctcttcgacg acccgacgaa gatcgcgagt
     9841 gttcagttca cagacccaat taccggggga gccatctccc cgacactgga ctgctggatg
     9901 cgccgacttg gcccagcgct gacgacgcac gcgacccgca aaacgggcaa ttccatcttc
     9961 gtcgtgttca gcgggactgg ggaaacctcg gtcggacgga gccggtttga gtgggaggcc
    10021 ggcgacgtgt tcgtcgcgcc gtcgtgggcg gtcgttgacc atcgcccata tgagacgtcc
    10081 aatctgttcg agatgaccga ccgcccgatc ctcgaggcgc tggggttgtt tcgctacgag
    10141 acgctcgaag tcgatgcatt ggtggaggtc tcatgaaagc gatcatcttc gaccgacacg
    10201 gcggaccgga ggtactgacc tacaccaacg tcgcggatcc atgccccggc ccgggcgagg
    10261 tgctgatctg cgtcggcgcg ctgacggtca atccggggcc ggatgtaatg acgagggagg
    10321 gcaccttcgg tctgccgggc ttcggcctac cgcacatcgg cggaagtgat ccggcgggcg
    10381 aggtggtcgc cgtcggcgac ggcgtcacga cgccccaggt cggcgatcga gtggtggtgt
    10441 accccgtgct gagctgcgga tcgtgcgact tctgccagcc cgggtccggt gaaaaccatt
    10501 gcgagaactg gagattgtgg ggtgcgcaga cgtggggcgg ccgtgccgag ctcgccaagg
    10561 tccctgcggc caacgtcgtg tcgctgccgg agagcgtgag ttgggaggcg gccgctgcgt
    10621 tgccggtgac gtacctgacc gcctggcatg gtttggtcga ctgcgcgggg gtcaaccatc
    10681 gcgacaccgt tcttgtgctg ggagccgcgg ggggagtggg cgtcgcggcg attcagaccc
    10741 gattccgcga gtaagccggg cagtagggat cttcgggtct gcggatgatc ttgaatgacg
    10801 ccgagacgtg ctggtccggc cgggtgcgcc gggttgcgcg tttcgatgtt gggttgtggg
    10861 ggcctgggcc acagtgcggc ctgaatatcg acggcgatga cggtgcgcgg cgacggcttt
    10921 cggtcgctgg tcagggtgcg ggcagtgcgc gcagccgagt gaggatcagg tcgagcaggt
    10981 cgcggcagcc gggttcgggc cgcagggtga gggtgcgggc gtgccggatc acccgcgccg
    11041 ggatcgcgat caggcggtgg cgcagggtgg cgatcatggc cttgccgccg tgaacgccgt
    11101 gaccggccac gatgtcggtg tcgtcgtcgg cgaggacggc agtgagttgg tgcagccagc
    11161 cggcgatgct ggcggcgagc agcgcgcccc acatccacgc ggtgttgacc tgcttgttgc
    11221 ccgatggcag gtgccgcagc gcggcgccgt gtttgctgtc gcggaagatg ttctccacgc
    11281 tggtgcggtg ccggtaccag tgctcgaccg cggcggcctg gtccggggtg ctcacgtcga
    11341 ggctggtcag gatgaacgaa tacgcgtaga ttgcgtcggc gtcggcgagc tcggccagcg
    11401 gcagcgcgcg ctggtcgggg tgcagggtgc cgcggcgccg cgaccgcggg tccgcggaga
    11461 cctggtccgg ccagagccgg acccgccgga tcagcagccg ggtgtccggt ggccagtcgg
    11521 cgggcgcgta gtccgagacg gcgacctgcg cgtcgggcat gtcgatcgct gcccgccaat
    11581 cggtttcggc gatgccggcc agtgcgcgcc acatggtggt gacgcgtttg gcgccgatcg
    11641 cgaacccgat ccccgcggtg tgcgcggcgc gggcgagttg cccggcgaag tagccggcgt
    11701 cggcgcgcag cgtgacccgc tcgcggcgga tccggcgcgg cagcccggcc agtgcgcggt
    11761 tgagcaggtc ctcggcggtg gcgcggggat cgtcgacccc ggagccgagg tcggcggcca
    11821 gcaccgtctc ggtctccgcc caggacgcca cgtgcgggcg cccgacccgc tgcccctgat
    11881 ggttgtaggc gacgccgtcc ttcttgcggc cgtacacctc gacatcggtg gcgtccatgt
    11941 cgatggtggc ctgcccctcg cacagcgccg ccgaccgctc ggcgggcagc agcgcgagca
    12001 tccgctcggt caccgcggca agcccggatt cggttgcgcg ccagtgctgt tcggtcatcc
    12061 gccgggccag cccggccgct gtggtggagg ccagccccgg caccggcgtc agcgcctgcc
    12121 cggcgacatc ggcgcggtgg cgatccagcc cgaccaggaa gtcctgcccg gccagctgcg
    12181 ccgccgccat cccgaccagc agctgcccgg gcccgaatcc ccgatcgcgc tgcttgatcg
    12241 gaccggtcgc cgcgttcagc aacccgatca catcgagccg ctcgcacagt tcggtcaccg
    12301 ccgccatccc ggcgttaccg gtcaccgacg gatccgccct gccgacccgc acccgccgac
    12361 gcttcccctt gcccttgtcg atgcgtatcc tgctcaccag atgggtgccc ttccgcgctg
    12421 ccggacgatg tgtcttctca acacgaatta tcccacgccg caacggcatc catcgctatc
    12481 ttccagcgat gtcacccact tactcgcgga atcgggtcag agggaggtgt tgaaattgtc
    12541 gtgcccgacc actgtcggct tccgcgcgag gccaaccgcg cgctggtaca cgtcataatg
    12601 gccgtgcggg atcggatgct gggatcagac aggggagtag cgtgagcctg aggttcgcct
    12661 tctatggccg agtgagcacg gaggacgctc aagacccgga ggcgagccgc agctggcaga
    12721 agcggcgtgc catagacctg atcactccgc acggcggagt cctcgccgct gactacttcg
    12781 atgtgggaca gagccgttcg ctgccgtgga agcgcagacc agaggcatca cgtctccttg
    12841 ccgatgtcac cgctagtgac cgtagcttcg atgccgtggt gatcggggaa cccgctcgcg
    12901 cgttctacgg gccgcagttc gcgctcactt tcccggtgct cacgcactac ggggtcggcc
    12961 tgtgggtgcc ggaggtcggc ggagcagttg accccggctc cgaggcgcac
//
LOCUS       DASWBF010000418         7182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1228345,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000418 DASWBF010000000
VERSION     DASWBF010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228345"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VF874_15520"
     CDS             <1..238
                     /locus_tag="VF874_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177420.1"
                     /translation="AEKDWAAGGQTNIDELTLACGPHNRDVKSGGWKTRKRPDGTTEW
                     IPPPHLDHGQRRTNNYFHPERMLPDDADDETDCS"
     gene            complement(250..1113)
                     /locus_tag="VF874_15525"
     CDS             complement(250..1113)
                     /locus_tag="VF874_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9177421.1"
                     /translation="MSRMSSAPETMTYTGVNDVALVADEWNRGGKSADGRPSILMLHG
                     GGQNRHSWKNTGQTLADQGFHVVALDSRGHGDSDHAPGADYALETLCGDVLRLIDAMD
                     GPVALIGASMGGLTGILAADAAGPATVTKLVLVDVVPRFEKSGSARIRDFMFSHVHGF
                     DSLEEAADAVAAYLPHRTRPRSPEGLKKNLRHRNGRWYWHWDPKLLTKPEDDPHLRVE
                     KLESAAIGLRIPILLIRGKLSDVVSVDGVKEFLDKVPTAEFVELSDAGHTAAGDDNDS
                     FTDAVVQFVTR"
     gene            1173..1781
                     /locus_tag="VF874_15530"
     CDS             1173..1781
                     /locus_tag="VF874_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9177422.1"
                     /translation="MPPRTRLADDERADIIDAAYACLSSPHPGPVSVAAILDAAGLST
                     RAFYRHFASKDELFLAMLKRDSEAVTRRLRKLAQETPGGPVAQLRAWIDCLLGLAYHP
                     RMRAHVVVLDSDEVRVAKGYREASAELRADRERVLADILRRGCDDGSFPLAEPDRDAA
                     AIHAVVDRAFAAPLFGLGPDRQLLVGYVLDFALRALGATQRR"
     gene            1835..2054
                     /locus_tag="VF874_15535"
                     /pseudo
     CDS             1835..2054
                     /locus_tag="VF874_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875405.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(2180..2491)
                     /locus_tag="VF874_15540"
     CDS             complement(2180..2491)
                     /locus_tag="VF874_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177423.1"
                     /translation="MTIVSLVTIHPNPGVKWDDVQKQLKKGCDLARKHSAENVTVLVT
                     MIGGAATNTIGILSTAEDWTKYGQIQQALMGDPEMQAAMLEAGQIATWETYVSQTIPD
                     M"
     gene            complement(3630..3797)
                     /locus_tag="VF874_15545"
     CDS             complement(3630..3797)
                     /locus_tag="VF874_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177424.1"
                     /translation="MAEQFDWEQEGYKRLHHLECENADLQREIAYLRKLLRELSEERK
                     WESPTVASQNV"
     gene            4084..4323
                     /locus_tag="VF874_15550"
                     /pseudo
     CDS             4084..4323
                     /locus_tag="VF874_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4360..5274
                     /locus_tag="VF874_15555"
     CDS             4360..5274
                     /locus_tag="VF874_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9177425.1"
                     /translation="MGEPGWVEVDAPSVTLRGLTWEPRHNADDAPIALCLHGFPDTAH
                     GWRLLAPKLAAAGWRIIAPFMRGYAPSTIPTDGSYHVAALMDDALRVLDAVGPTGRDV
                     VIGRDWGAIAGAGLAAMPDSPFTRAVIMSVPPAASFRPRGRVPDAGRLAAQLPRQLAH
                     SWYIMYFQLPLLPERSASWVPRRLWRQWSPGYDAAEDLRHVDAAIGTPESWRAALGPY
                     RATVRNTQPPSRYAELHRHWLAPPTLPTLYLHGTDDGCTTPAYSEWVANILPPGSDLH
                     LVEHAGHFLQLEQPEIVAGHILDFVGRV"
     gene            complement(5271..6077)
                     /locus_tag="VF874_15560"
     CDS             complement(5271..6077)
                     /locus_tag="VF874_15560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019665295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177426.1"
                     /translation="MTLPPPAEDRAALITGASSGIGEAIARELARRGHRVVLVARRVE
                     QLEALAGELGARAHPLPADLSERTERAALPGRVAALGVSVDVLINNAGLATVGPIAKA
                     VPEKELNLVEVDVAAVVDLCCRFVPGMVERRRGAVLNVASTGAFGPLPGQASYGAAKA
                     FVLSYTDSLRGELRGTGVTATTLCPGPVKTGFGEVAGISDEEAEAALPRVMWKTADEV
                     ARAGIDGLAAGKGVVVPGGFNRVAGYLYRVAPRDVLLPLLAARHPGLKNG"
     gene            6082..>7182
                     /locus_tag="VF874_15565"
     CDS             6082..>7182
                     /locus_tag="VF874_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1298 domain-containing protein"
                     /protein_id="HEX9177427.1"
                     /translation="MTVALTAADAQFYWMSAKVPNDQLLLYAFDGEPADLEDAVAQVI
                     RRARRCPDLSVRVDDRCALTYPAWVRRDVAPDQVVVHGPVAGWQECLDAVAALAEHQL
                     DMRDTTWRLHIFGPVPDVPGASAPSTVTVLQMGHALGDGVRSSALSAVLFGRAANVPG
                     IERRRRGCLITRGVAAARAHRRLEYDTTAGLVPRPAPLRPALSINGKPEGGRAIRSFA
                     RRRSELSGRTVTVAVLAAVSGALSAYLRDRDEDPSTLAAEVPMAKHRVRQANNYFGNV
                     GVGLYPQLSFDQRADRIAVELGERRRRAEHPAFAAGDRSLASVPAPLLRWGVSQFDVD
                     VRSPVVIGNTVVSSMNRGPADLSFGGRPVVLTA"
BASE COUNT         1167 a         2426 c         2332 g         1257 t
ORIGIN      
        1 cgccgaaaaa gactgggccg ccggcgggca gaccaacatc gacgaactca ccctggcctg
       61 cggaccccac aaccgcgacg tcaaaagcgg cggctggaaa acgaggaaac gcccagacgg
      121 caccaccgag tggattcccc cgccgcatct ggaccacggc caacgccgca ccaacaacta
      181 cttccacccg gaacgcatgc tgcccgacga cgcagatgac gaaacagact gtagctgaag
      241 gtatttcagt cagcgggtga cgaattgcac gacggcgtcg gtgaacgagt cgttgtcgtc
      301 gccggcggcg gtgtggccgg cgtcggagag ttcgacgaac tcggcggtgg gcaccttgtc
      361 gaggaactcc ttgacgccgt cgacgctcac cacgtcggaa agcttgccgc ggatgagcag
      421 aatcggaatg cgaaggccga tggcggcgct ctccagcttc tcgacacgaa gatgcgggtc
      481 gtcctcgggc ttggtcagca gcttcgggtc ccagtgccag taccagcggc cgttgcgatg
      541 acgcaggttc ttcttcagcc cctcggggct gcgtggccgg gttcgatgcg gcagataagc
      601 cgccacggcg tcggccgctt cctccagcga gtcgaacccg tgcacgtggc tgaacatgaa
      661 atcccggatc cgggcactgc cgctcttctc gaaccgcggc acgacatcga cgagaaccag
      721 cttggtgacg gtcgccgggc cggcggcgtc ggcggccaga attccggtca ggccgcccat
      781 gctggcaccg atcaacgcga cgggtccgtc catggcgtcg atgagtcgca ggacatcgcc
      841 gcacaatgtc tcgagtgcgt aatcggctcc gggcgcgtgg tcactgtcgc cgtgcccccg
      901 agagtccagc gccaccacgt ggaaaccctg atcggcgaga gtctgaccgg tgttcttcca
      961 ggagtgccga ttctgaccgc caccgtgcag catcagaatc gagggccgcc cgtcggccga
     1021 cttgccgcca cggttccact catcagcgac gagcgccacg tcgttgacgc ccgtataggt
     1081 catcgtctca ggtgcgctgc tcatccggct cacggtagcc gacgtgagat cggcgttttc
     1141 gctcacgccc tccggtccta caatcggcgg tcgtgccacc gcggacacgg ctcgccgacg
     1201 acgaacgtgc ggacatcatc gatgctgcgt acgcgtgcct gtcgtcgcca catccgggac
     1261 ccgtgtcggt ggcggcgatc ctggacgctg cgggcttgtc gacccgggcc ttctaccgcc
     1321 acttcgcctc gaaagacgag ttgttcctgg cgatgctgaa gcgggacagc gaggcggtta
     1381 cacgccggct gaggaaactg gcgcaggaaa ctccgggcgg cccggtggcg cagctgaggg
     1441 catggatcga ctgccttctg ggactggcgt atcacccacg gatgcgcgcg catgtcgtcg
     1501 tgctggactc tgacgaggtg cgggtcgcca agggctaccg ggaggccagc gccgagctac
     1561 gcgccgatcg ggagcgggtc ctcgccgaca ttctgcggcg gggttgcgac gacggctcct
     1621 ttccgctcgc cgagcccgac cgtgacgcgg ccgccatcca cgccgtcgtg gatcgcgcgt
     1681 tcgccgcacc cctgttcggc ctcggtcccg atcggcagct tctcgtcggg tacgtccttg
     1741 acttcgcgct tcgtgcgctc ggtgcgacgc agcggcgatg agttccggcc gccgcagcgg
     1801 tcgacactgg tagccgacca cgaaggagac cgcgatgccg tcgatcactc cgtcgctttg
     1861 gttcgacaac gacctcgagc aggccgccga gttttacacg gccgtgttcc cgaactcgag
     1921 gatcgaggga atgaaccgcc acacggccga tgagccgctt gcgcgaagag gaaaagatga
     1981 agcgggcccg ggaaagcccg gtgaaggtgt cgggaagctt cgtgctcgac ggcacccggt
     2041 tcgtcgggat aaacgtacag gttgggtaca caagcagctt aatggtgctg gggcacttca
     2101 tatcccaccc cgctggcatt ccagaatgac cggtaacgac gcgatgggtg tcgccgctcc
     2161 ggcactgtct ggagccttgt tacatgtccg ggatcgtctg gctgacgtac gtctcccagg
     2221 tggcgatctg gccagcctcg agcatggcgg cctgcatctc aggatcgccc atcagcgcct
     2281 gctggatttg gccgtacttc gtccaatcct cggcagtgga gaggatgccg atggtgttgg
     2341 tcgccgcccc cccgatcatg gtcacgagga cggtcacgtt ctcggcgcta tgcttacggg
     2401 cgaggtcaca ccccttcttg agttgcttct gcacatcatc ccacttgacg cccgggtttg
     2461 ggtggatcgt tactaacgag acgatggtca tgttggccct ccctaatgcg tgaaggttgt
     2521 tggctgacta atggcctgac tgtaggccga tggcagcgat gtcgcgacaa cttggcaacg
     2581 agatcgcaaa aacaaatcaa cctggaaata tgctgggaaa taactgcggg gcaaaatacc
     2641 cgcccggcaa acggacggag ggtcacccca gacgccgatc acgcggagcg ccagcgcgtg
     2701 atccgagaaa gctggaccgg ctggacaacg gggagccacc atgattcggg atcaacggcg
     2761 gcccgcagtt cccgttcacc gtagccgtgt cgttcatggt gaactgcgcg acgggggcac
     2821 atcaagccca ggtcggagcg cttacgtctg cggtcagttc gcacgtggca ggtcgccgaa
     2881 tgttgctcag gtcgtctctt aatgctcgcc atcgccaggc ctctccttgt ctatggctcg
     2941 ttggaggtct atggctcgtt ggagttggtc ccgtgccttg tcgcccggct ccagcaggta
     3001 gtcggcgtag gcgagccgcc gcatcacacc gggcttcttg aaaagcgatt ctctgcaaca
     3061 cctttcgatg ctgcttcgcc gccgcagcca gagacgaggt cggaatcccc agttcgatcc
     3121 gccttgaact tgtccgccga cggttgtgca ttgcaaggat gcgcgactgc tgtcttgatt
     3181 ttctcggggt cgggtgcgcc gtcgtcgctg agaaggtcgg cgacttgtgc ccgggtgcgg
     3241 cgggttcttc ttcggccggc tgattctgct gatcgttgtc attcatcggt ccgcgcgacc
     3301 gatcagcagc gccgagtcct cgagggtgag tggtttgcgg gtggaaggat cttgctaacg
     3361 tggttggggc cccggcgggt cagggcggga agacaattta tccatcgact ccacctgagg
     3421 ggtcccgtcg gggcttatgc gggactgtac gcctgactaa ttccccgctc agctgattcg
     3481 atttatacca aaggtataaa tcgtcgcact tcccctaggg ctcgcgggcc agcgcgagca
     3541 cgtccttggg tggggggata cccccttcga tgcggaagcg cggcagacac aggctttgcc
     3601 ggtttgcaca cacagagcga gagttacagc tagacgtttt ggcttgcaac ggtgggggat
     3661 tcccactttc tctcttctga tagctctctc aagagctttc gcaggtaggc aatttcgcgc
     3721 tgcaggtcgg cgttctcgca ttccaagtga tgcaaccgct tgtagccttc ctgctcccag
     3781 tcgaattgct ccgccatttt gcggcctcct ttggatcggc ggtacacggt gtactcaagg
     3841 tgccgcaaca atgcgggcgt gtctgtccgc caatcggacc gcagagcgga tgatttcaat
     3901 gtcccccgat cgggggactc gctgagccgc ggcccgctgc cgcagccggt tgagtccgca
     3961 gaaagtcccc aagagccccg aaactagccc atccttgcca acgctgctga tcggcgttct
     4021 cccagttcaa tgccgtttta caaacctgcc ccgatcttgc caatcgctag gaggtagttc
     4081 atggtgaact gcaaagacca ggacgaggtc gactactact gggatcggct gctggccggc
     4141 ggccaggagt cgcagtgcgg ctggctgaag gatcggttcg gtgtcagctg gcagatcgtc
     4201 cccgaccgcc tcttcgaact gctcaccgat cccgacccgg cccgcgccgc cgcgaccaag
     4261 gccatgctcg gcatgcgcaa gatcgtcatc gctgaactcg aggccgctgc cgacgcagcc
     4321 tgaccgcggg gcccgaccgc agccggttaa gctgccgccg tgggcgagcc gggttgggtc
     4381 gaagtcgacg caccctcggt gaccctgcgc gggttgacgt gggagccccg ccacaacgcc
     4441 gacgacgcgc caatcgcgtt gtgcctgcac ggttttccgg acaccgcgca cgggtggcgg
     4501 ctgctcgcgc cgaagctagc ggcggctggc tggcgcatca tcgcgccctt catgcgcggc
     4561 tacgcgccgt cgaccatccc gacggacggc agctatcacg tcgccgcgtt gatggacgac
     4621 gcgcttcggg tactcgacgc agtagggccg accggtcgtg acgtggtcat cggccgcgac
     4681 tggggcgcca tcgccggggc gggtctggcc gcgatgcccg acagcccgtt caccagggcg
     4741 gtcatcatgt ccgtgccgcc agcagcgtcc ttccggccgc gcggccgcgt ccccgatgcc
     4801 gggcgcctgg ccgcgcagct tccccgccag ctcgcccaca gctggtacat catgtacttc
     4861 cagctgccgc tgttgcccga gcgctccgcg tcatgggtcc cgcgccggct ctggcggcag
     4921 tggtcgcccg gctacgacgc cgccgaggac ctgcgccatg tcgacgcggc gatcggcaca
     4981 cccgagagct ggcgcgcggc actgggtccg taccgcgcca ccgtgcgcaa tacccagcca
     5041 ccgtcgcggt acgccgagct ccaccggcat tggctcgcgc cgccgaccct gcccacgctg
     5101 tatctgcacg gcaccgacga cggctgcacc acacccgcct actccgaatg ggtcgcgaac
     5161 attctgccgc ccggcagcga cctgcacctc gtcgagcacg caggccattt cctccaactc
     5221 gaacaacccg agatcgtcgc cggtcacatc ctcgacttcg tcggccgggt ttagccgttc
     5281 ttcagaccgg ggtgacgggc ggccagcaga ggcagcagca cgtcccgcgg cgccacccgg
     5341 tacagatatc cggcgacgcg gttgaatcca ccgggcacca ccacgccctt gcccgccgcc
     5401 aagccgtcga tacccgcgcg cgccacttcg tcggcggtct tccacatcac ccgcggcagc
     5461 gcggcctcgg cctcctcgtc gctgatgccg gcgacctcgc cgaagccggt cttcaccgga
     5521 ccggggcaca acgtggtcgc ggtgacaccg gtcccccgca gctcgcccct tagggaatcg
     5581 gtgtacgaca acacgaatgc cttcgcggca ccgtacgacg cctgcccggg cagcgggccg
     5641 aacgcgccgg tcgatgccac gttgagcacc gcacctcggc gtcgctccac catgcccggc
     5701 acgaacctgc agcacaggtc cacgacagcg gcgacgtcga cctccacgag gttaagttct
     5761 ttctctggaa cggctttcgc gatcggcccg acagtcgcca agccggcgtt gttgatcagg
     5821 acgtcgacgc tcacgccgag agcggcgact cggcccggca gcgcggcgcg ttcggtgcgc
     5881 tccgacaggt cagccggcag cgggtgcgcc cgcgcgccga gctcaccggc gagcgcctcg
     5941 agttgctcca cgcgtcgggc caccaacacg acccgatggc cgcggcgggc cagttcgcgt
     6001 gcgatcgcct cgccgatacc tgacgacgcg cccgtgatca gcgcggcacg gtcttctgcc
     6061 ggtggtggaa gagtcattcg aatgacggta gccctgaccg ccgccgatgc acagttctac
     6121 tggatgtcgg ccaaggttcc caacgaccag cttctgcttt acgcgttcga cggcgaaccc
     6181 gctgatctgg aagacgccgt cgcgcaagtc attcgacgcg cccggcgctg cccggacttg
     6241 tcagtccgcg tcgacgaccg gtgtgcgctg acgtatccgg cctgggtacg tcgcgacgtc
     6301 gcacccgacc aagtcgtcgt gcacggcccc gtggcgggct ggcaggaatg tcttgacgcg
     6361 gtggccgcgc tggccgagca ccagctcgac atgcgggaca cgacgtggcg gctgcacatc
     6421 ttcggtccgg tgccggacgt gcccggcgct tcggcgccgt cgaccgtcac ggtcctgcag
     6481 atgggccacg ccctgggcga cggggtgcgg tcgtccgcgc tctcggccgt gctgttcggg
     6541 cgggcggcga acgtgccggg catcgagcgg cgtcgaaggg gatgccttat tacgcgcggc
     6601 gtcgccgcgg cgcgggcgca ccggcgactg gagtacgaca cgaccgccgg cctcgtcccg
     6661 cggccggcgc cactgcggcc ggcgctgagc atcaacggca aacccgaagg cgggcgcgcg
     6721 atccgcagct tcgcgcggcg ccggtccgag ctgtcggggc gcaccgtcac ggtcgcggtt
     6781 ctggccgcgg tgtccggcgc gctgtcggcg tacttgcgcg accgcgacga ggatccgtcg
     6841 acactcgccg ccgaagtacc gatggccaaa caccgtgtgc ggcaagccaa caactacttc
     6901 ggcaacgtcg gtgtcgggct gtacccacag ctgtcgttcg accagcgggc ggatcggatc
     6961 gccgtcgaac taggcgagcg tcggcgccgc gccgaacacc cggccttcgc ggcgggcgac
     7021 aggtcactgg catcggtccc agcgccgttg ttgcgttggg gtgtaagtca attcgacgtc
     7081 gatgttcgtt cgcccgtcgt catcggcaac accgtggtct ccagcatgaa ccgcggcccg
     7141 gccgatctat ccttcggcgg ccggcctgtc gtgctgaccg cc
//
LOCUS       DASWBF010000419        10297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1087345,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000419 DASWBF010000000
VERSION     DASWBF010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087345"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VF874_15570"
     CDS             complement(<1..255)
                     /locus_tag="VF874_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9177428.1"
                     /translation="MSAYRTVVVGTDGSDSSLRAVDRAGAIAAQSGAKVYVASAYFPQ
                     HDDPRAADVLKDESYKTTGSAPIYAILKEAHERATAAGAKD"
     gene            544..2103
                     /locus_tag="VF874_15575"
     CDS             544..2103
                     /locus_tag="VF874_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177429.1"
                     /translation="MSISLTPQRVAGVPEESGPSAQRSRSFDAALVAVLAAGVSLAGA
                     ARPSLWFDEAATISASTRSLTELWQMLGKIDAVHGLYYLLMHGWFAVFPATEFWCRVP
                     SGLAVGGAAAGVVVLTKRFSSRSVAVCAGIVFAILPRTTWAGIEARSYAFSALAAVWL
                     TVLLVAAVRRNSAALWLFYGLGLLLSVLLNLNLVLLVPVYAAVVAMLQPAKREVIWWL
                     ATTAAVTAAVIPFVVFAHGQIYQVGWIFRLGSRTFGDVLVQQYFDDSLVFALAAGAVL
                     VLAILFWFRGIRRPDRDTLRLLLIAAAWIAVPTAVLLVYSALFKPIYYPRYLYFTTPA
                     AAIVLASCITTITTKPFAVAGLLFTLAAVAAPNYLLVQRGPYAKEGMDYSQVADVIST
                     HAAEGDCLVLDITTAWKPGPIRPLTAARQALYQRLNDPGRGTTAVQRHWLWDGHLAIQ
                     AVAKRLTGCRVIWTISERDKTRPEHERGDSVPPGPRLSRTPDFQIPHKMGFRIVERWQ
                     FNFAQVTKSTR"
     gene            2117..2769
                     /locus_tag="VF874_15580"
                     /pseudo
     CDS             2117..2769
                     /locus_tag="VF874_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017682031.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
     gene            complement(2766..3017)
                     /locus_tag="VF874_15585"
     CDS             complement(2766..3017)
                     /locus_tag="VF874_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177430.1"
                     /translation="MGLVKDTRAAVLRIEAERALQQGRWVFTPTLTTTTGYDAPGSIA
                     GWAEMIEAIEEVGWTLYHWSASLDIHGNPQAYPLFRRRG"
     gene            3186..5237
                     /locus_tag="VF874_15590"
     CDS             3186..5237
                     /locus_tag="VF874_15590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX9177431.1"
                     /translation="MRLAVRVLVAALSAGVVLSGCADSSPERPARPNFGTWGIDTADM
                     DRTVRPGDDFFDYVLGSWLKTAQIPADKLCAGVGLEIQNQLDDDLKSVVEGAAAENAS
                     DGEVSQQIGDLYSSYMDEATLNARGVEPIRPHLAAIDGVTDRAGLDSVLVRLNMTSTA
                     TPFDIGVAIDPTTPTRYVATWEQGGLSLGERDYYLNQDAESVDLRDKFIAHVAKLLGL
                     AGYADARAQAEQVLGLETKLAEVQWPVEDTRDVQKTNNIMSRSDVEKLGAGAPLSGIF
                     DARELPPGTDFQIGMPDVLAKTAQLFASEPVDAWKAYLRYQLLSAYGPRLSAPFSEEV
                     FDFYGRVLSGQQERAPRWKRGVSWVSDALGDEVGERYVAAHFTQQTRDQALALVENLR
                     RAYSARIDDAAWMDPATKKEAQVKLAALVAKIGYPDKWRSYDSVAIRPDDLIANVESL
                     EAWSWDESLNKLGKPIDRTEWGMTPQTNNAYYSAQLNDIAFPAAILQPPYFDPAADAA
                     ANYGAIGATIGHEMSHAFDDQGRKSDATGALRDWWTPEDAERYNREADRLVAQFDAYE
                     PLPGIHINGAQTIGENIADLAGLRIAYDAYRLSLGGNEAPVIDGLTGDQRFFIAYAAT
                     WKNTCRPETERNLLMTDVHSPERYRVNGIVRNMDEWYAAFGVTQGDKLYLKPEDRVRV
                     W"
     gene            complement(5243..5313)
                     /locus_tag="VF874_15595"
     tRNA            complement(5243..5313)
                     /locus_tag="VF874_15595"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5279..5281),aa:Cys,seq:gca)
     gene            5408..6139
                     /locus_tag="VF874_15600"
     CDS             5408..6139
                     /locus_tag="VF874_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005072297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX9177432.1"
                     /translation="MWLAADPAFFHGYGQGTRQLVELVVAFGLTALVGLEREIQGKSA
                     GLRTQAIVGTSAALILIISKYGFGDVLVAGTVVVDPSRVAAQIVSGIGFLGAGLIIMR
                     RGAVHGLTTAAAIWECAAIGMAAGAGLLLLAVAVTALHFVIIFAFGPLARRLTNRLRG
                     TIRVYVTYQDEHGVLRQLLRACENHGWQLMSVETRRALDTPSAEPGDVGVLMTISGAG
                     VLRSTRVLATVDGVKSVRQLEDETE"
     gene            complement(6140..7009)
                     /locus_tag="VF874_15605"
     CDS             complement(6140..7009)
                     /locus_tag="VF874_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177433.1"
                     /translation="MDAFSAGRLTRSLDPLHSTAYFLPDVTSQFGALGMKGRTPYFAT
                     RSAPMGAVGAGVVAATFYNFNPELVGASIPAAWQMASPQTVTEMRYAIVEQALPGVLG
                     TSLTESAELSRAAEILRRVAEAIPNADGRPLYAGHAELPWPRSPHARLWHAITLLREY
                     RGDGHIAALITYELNGLEALITHTASEIGFTVEFARRLRGWSEEKWAAATDRLRQRRL
                     LDDSGALTTSGLQLRTEIEDLTDRLAAEPWRTLADDDAAELERFAAPVRQAVLGAGVF
                     PAGAFAARYGQHR"
     gene            complement(7020..8417)
                     /locus_tag="VF874_15610"
     CDS             complement(7020..8417)
                     /locus_tag="VF874_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9177434.1"
                     /translation="MSEATVATGSWRELLGRDRLRTSTLLAGGVALYATNEFLTISLL
                     PSTIADIGGQRFYAWVVSVYLVGSVVAATAVNPLLMRRGPRASYLLVLTLFGAGTVGC
                     AIAPTMELLLVARTIQGAAGGMLAGLGYAVINAALPNWLWTRASALVSAMWGVSVIIG
                     PAMGGVFAQFGIWRWAFGSLAIATMAMAALVPGALPSRAKSDVAPATLTRIPVWSLLL
                     LGLAALAVSLAGVQRTVSVTAGLLVLGVALVGCFLLVDRRARATVLPRAAFGPGPLKW
                     MYVSVGLLMAATMVDMYVPLFGQRLAHMTPVAAGFLGAVLAVGWTLSEIVSASANRAR
                     LVVRMVAVAPAIMAIGLGLAALTVDGGPTWPVIVVWVVALFVAGTGVGIAWPHLSAWI
                     MGAVDDPVEGGAAAAAINTVQLISGAFGAGLAGVVVNMVPGGDLAAARWLFGMFAALA
                     AIGVVATCRSNPAAK"
     gene            complement(8457..>10297)
                     /gene="uvrB"
                     /locus_tag="VF874_15615"
     CDS             complement(8457..>10297)
                     /gene="uvrB"
                     /locus_tag="VF874_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX9177435.1"
                     /translation="AVEYFVSYYDYYQPEAYIAQTDTYIEKDSSINDDVERLRHSATS
                     SLLSRRDVVVVASVSCIYGLGTPQSYLDRSVEVQVGMEVPRDALLRLLVDVQYTRNDL
                     SFTRGSFRVRGDTVEIIPSYEELAVRIEFFGDEIEALYYLHPLTGEVIRKVDSLRIFP
                     ATHYVAGPERMAHAISTIEQELAERLAELERQGKLLEAQRLRMRTNYDVEMMRQVGFC
                     SGIENYSRHIDARPAGSAPATLLDYFPEDFLLVIDESHVTVPQIGGMYEGDISRKRNL
                     VDYGFRLPSAVDNRPLTWEEFADRIGQTVYLSATPGPYELSQSSGEFVEQVIRPTGLV
                     DPKVVVKPTKGQIDDLIGEIRKRAGADERVLVTTLTKKMAEDLTDYLLEMGIRVRYLH
                     SEVDTLRRVELLRQLRLGDYDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSSRSL
                     IQTIGRAARNVSGEVHMYADTVTDSMKEAIDETERRRAKQIAYNEERGIDPKPLRKKI
                     ADILDQVYREADDSDRVEVGGSGRNVSRGRRAQGEPGRAVSAGIYEGRDTSNMPRAEL
                     ADLIKDLTAQMMAAARDLQFELAARIRDEIADLKKELRGMDAAGLK"
BASE COUNT         1735 a         3428 c         3363 g         1771 t
ORIGIN      
        1 gtccttggcg cccgcagccg tggcccgttc gtgggcttcc ttcaggatcg cgtatatcgg
       61 ggccgagccc gtcgtcttgt agctctcgtc cttgagcacg tcggctgccc gcggatcgtc
      121 atgctgggga aagtacgccg aggcgacgta cactttcgcg cccgattggg cggcaatggc
      181 acccgcccgg tcaaccgcac gcagcgatga atccgagccg tcggtcccga ccaccacggt
      241 tcgataggcg ctcatttacc ctcccactcg ttggttgcta ggccaacccc agacactagc
      301 cgcttggcct gcaataacgg gagcgattgg cgacacagtt gcgcttcctc tgtgtcaata
      361 tcgggcttct accggccgca atgggtttct ccgtgccgct cgtgaccttc ccgacacgcg
      421 cgtcacgatg gggcgccgtt cactgaacgg gattgagctt cactgtcatt cggatcggca
      481 ggcggcttcg ctcgcggcgc gctacggttt gtcgcatcgc tggatatggg aacaggtgac
      541 tgcgtgagca tttcgctgac tccgcagcga gtcgcgggcg tgccggagga gtccgggccg
      601 tcagctcagc gcagtcggtc gttcgacgcg gcgctcgtcg cggtgctcgc cgcgggcgtt
      661 tcactggcag gggctgccag gccgtcgctc tggttcgacg aggctgcgac catttcggcg
      721 tcgacgcgat cgttgaccga gctgtggcag atgctcggca agatcgacgc ggtccacggc
      781 ctctactacc tgctgatgca cggctggttc gcggtctttc cggcgaccga gttctggtgc
      841 cgcgtcccca gcggacttgc cgtcggcggc gcggccgctg gggtggtcgt cctgacgaag
      901 cggttctctt cccgctccgt ggccgtctgc gccgggatcg tgttcgcgat cctgcctcgc
      961 accacgtggg cgggtatcga ggctcgctct tatgcgttct cggcgctggc ggccgtgtgg
     1021 ttgacggttc tgctcgtcgc ggcggttcga cgcaacagcg cggcgctgtg gctgttctac
     1081 gggctcggct tgctgctgtc ggttctcctc aacctaaacc tggtgttact cgtcccggtc
     1141 tacgccgctg tagtggcgat gctgcaaccc gcaaaacgcg aggtgatctg gtggctcgcg
     1201 acgacggctg cggtgaccgc ggctgtgatt ccgtttgtgg tgttcgccca cgggcagatc
     1261 tatcaggtgg ggtggatatt tcggctcgga tcaaggactt tcggtgatgt cctcgtgcag
     1321 cagtacttcg acgacagcct ggtgttcgcg ctcgctgccg gagcggtgtt ggttctggcg
     1381 atcctgttct ggttcagagg catccgccgt ccggaccgag acacgttgcg gctgttgctc
     1441 attgctgccg cctggatcgc ggttcccacc gccgtgctcc tcgtctactc ggcgctgttc
     1501 aagccgatct actaccctcg ctacctgtat ttcaccacgc ctgcagcggc catcgtgctg
     1561 gcatcgtgca tcaccacgat tacgaccaag ccgttcgccg tcgccggtct gctgttcaca
     1621 ttagctgccg tcgctgcccc gaattacctt ctcgtgcagc gtggcccgta cgcgaaagag
     1681 ggcatggact acagccaggt cgccgatgtc atcagtaccc atgcggcaga aggcgattgc
     1741 ctggtcttgg acatcacgac ggcgtggaag ccggggccga tccggccact gaccgcggca
     1801 cgacaggcgt tatatcagcg cctgaacgat cctggccggg gtacgacggc ggttcaacga
     1861 cactggttgt gggacgggca tttggcgata caggccgtcg cgaagcggct aaccggctgc
     1921 cgtgtcatct ggacgatctc cgagcgcgac aagacccgac cggaacatga gcgcggcgac
     1981 tcggtgccgc ccggtccacg gctcagtcgg acgcctgatt tccagatacc gcacaagatg
     2041 gggttccgca ttgtcgagcg gtggcagttc aacttcgccc aggtgacgaa gtcgacccga
     2101 tagggggtga tcttggatgg ccgcgccggg ggcacaggag ttcgtgccgc agacagacga
     2161 tttggccgac ctcgctgcag ccgttcaggc gtgccgcggt tgcgacctgt atcaggacgc
     2221 ggaccaggcg gtgttcggtg cggggtcgtc aaccgcccag gtcatgctcg tcgggagcag
     2281 cccggcgacc aggaggaccg cgcgggcctg ccgttcgtcg gacccgcggg caagttgctc
     2341 gacaaggcac tggttgccgc gggcatcgac cgggaccggg tgtacgtcac caacgcggtc
     2401 aagcacttca agttcacgcg gcccgaacgg ggaaagcgcc gcattcacaa gacaccgagt
     2461 cggaccgagg tggtggcatg ccgcccgtgg cttattgccg agttgaccac ggtgcagccc
     2521 gatctcgtcg tacttctcgg ggcgactgcg gcaaagtcgt tgctgggcaa tgactttcgg
     2581 ctcactgctc accgcgggga ggcgctgcgg ctgccggcgg acgacaaagc ggtccggctc
     2641 gacttcggcc cgcaagtggt cgcgaccgtg cacccgtctt cggtgctgcg cgggcccccg
     2701 gaggatcgcg agaccgcgtt cgacgagctg gtggctgatc tgcggttcgc ggccgggctg
     2761 gtgacctaac cgcgccggcg gaacagcggg tacgcctgtg ggttgccgtg gatgtcgagc
     2821 gacgccgacc agtggtacag cgtccagccg acttcctcga tggcttcgat catctcggcc
     2881 cagccggcga tcgacccggg cgcgtcgtaa ccggtggtcg tcgtgagcgt cggggtgaat
     2941 acccaacggc cttgctgcag cgcgcgctca gcttcgatgc gaagcactgc tgctcgggtg
     3001 tctttgacaa gccccatagc cggatggtag ctacacggcg cttgctgcag cgtcagcgaa
     3061 acggagctgt tgccagatcg tggcgttccg tgccacgcgt gaccggggcc cttctcgaac
     3121 ccgggcgccg accgaccact gctgcgagta tgctccccgg cgttcgcaca aaggtggtcg
     3181 gtgggatgcg cttagcggtt cgtgtgctgg ttgcggctct gtctgcgggg gtcgtgctgt
     3241 cgggatgtgc cgactcgtca cccgaacggc ccgccaggcc gaatttcggc acgtggggca
     3301 tcgacacggc ggacatggac aggacggttc gacccggcga tgacttcttc gactatgtcc
     3361 tcggctcctg gctgaagaca gcgcagatcc ccgccgacaa actctgcgcc ggtgtcggcc
     3421 tggagataca gaaccaactc gacgacgacc tcaagtcggt cgtcgagggt gccgccgccg
     3481 agaacgcatc ggacggtgag gtatcccagc agataggcga cctctactcg tcgtacatgg
     3541 acgaagccac gttgaacgct cggggcgtcg agccgataag accccacctg gcggcgatcg
     3601 acggtgtgac ggaccgagcc ggcctcgact ccgtgctcgt gagactcaac atgacgtcga
     3661 ccgcgacacc gttcgacatc ggtgtcgcca tcgatccgac cactcccact cggtatgtcg
     3721 ccacgtggga gcaaggtggc ctttctctgg gtgagcgcga ttactacttg aaccaagacg
     3781 ctgagtcagt tgacctgcga gacaagttca tcgcccatgt tgcgaagctg ctcggcctcg
     3841 cgggctatgc ggatgcgcgg gcacaggcag aacaagtcct gggcctggag accaagttgg
     3901 cggaggtcca atggccggta gaagataccc gtgacgtcca gaagaccaac aacatcatgt
     3961 cgcgctcgga tgtcgaaaag ctcggcgcgg gagcccctct gagcgggatc ttcgacgcgc
     4021 gcgaacttcc ccccgggacc gacttccaaa tcggcatgcc cgatgtgctg gccaagaccg
     4081 cacagctgtt tgccagcgag ccggtggacg cctggaaagc gtatctgcgc tatcagctgc
     4141 tgagtgctta cggcccgcgg ttgtccgcgc cgttctccga ggaggtgttc gacttctacg
     4201 gccgcgtgtt gtccggccaa caggagcgag ctccgcgatg gaagcgcggt gtttcgtggg
     4261 tgagtgacgc gctgggtgat gaggttggcg agcgttatgt cgccgcgcat ttcacgcagc
     4321 agactcggga ccaggcgctg gcgctggtcg agaacttgcg cagagcatat tccgcgcgga
     4381 tcgatgacgc ggcgtggatg gatcccgcaa ccaagaagga agcgcaggtc aagctggcgg
     4441 ccctggtggc gaagatcggc tacccggaca agtggcgatc gtacgactcg gtcgcgatcc
     4501 ggcccgacga tctcatcgcc aacgtcgaaa gcctcgaggc gtggtcgtgg gacgagtccc
     4561 taaacaaact cggcaagccg atcgaccgaa ccgaatgggg tatgaccccg cagacgaaca
     4621 acgcctacta ctctgcccaa ctcaacgaca tcgctttccc cgcagcgata ttgcagccac
     4681 cgtatttcga tccggcagcc gatgcggcgg ccaactacgg cgccatcggc gccaccattg
     4741 gccatgagat gagccacgca ttcgacgacc agggccgcaa gagcgatgcg accggtgcgc
     4801 tgcgcgactg gtggacgccc gaggatgccg agcgctacaa ccgggaggcc gacaggctcg
     4861 tggcgcagtt cgacgcatac gagccgctac cgggcataca catcaatggg gcgcagacga
     4921 tcggggagaa catcgcggat ctggccggac ttcgaatcgc ctacgacgcg tacagactct
     4981 cgttgggcgg caacgaggca ccggtgattg acggcctcac cggggaccag cgcttcttca
     5041 ttgcctatgc ggcaacgtgg aagaacacct gccgcccgga aaccgaacgc aacttgttga
     5101 tgaccgatgt gcacagcccc gagaggtacc gagtgaacgg gatcgtccgc aacatggacg
     5161 agtggtatgc cgcgttcggc gtcacgcaag gcgacaagct ctatctcaag cctgaagacc
     5221 gagtcagggt gtggtgaaac agagcgagtg acgggactcg aacccgtgta atcggctttg
     5281 caggccggtg cctgaccgct cagccacacc cgcactgcac agcagaatcg catggttgtc
     5341 ggccgaggaa ccagcgtttc cctcacgctg attccggctg acggctggca cagtaagcac
     5401 catgcagatg tggctcgcgg ccgacccggc cttcttccac ggctacgggc agggcacccg
     5461 tcaactggtc gagctggtgg tcgcgttcgg tctgaccgct ctggtcggcc tcgagcgcga
     5521 aatccaaggt aagagcgccg gccttcgcac gcaggcgatc gtcggcacgt ccgctgcgct
     5581 gatcctgatc atcagtaagt acgggttcgg cgacgtcctc gtggctggaa ccgtggtggt
     5641 cgacccgtct cgcgttgccg cgcagatcgt ctcgggcatc gggttcctcg gcgcgggtct
     5701 gatcatcatg cggcggggcg ccgtgcatgg tctgaccacc gccgcggcga tctgggaatg
     5761 cgccgcgatc ggcatggcgg ccggcgcggg cctcctgctg ctcgcggtcg cggtgacagc
     5821 gctgcacttc gtgatcatct tcgccttcgg tcccctcgcc cgccggctga cgaaccggct
     5881 gcgcggcacg attcgcgtgt acgtgaccta ccaggacgag cacggtgtgt tgcgtcagct
     5941 tctgcgcgcc tgcgagaacc acggctggca gctcatgtcg gtcgaaaccc gacgtgctct
     6001 cgacacaccg tccgcggagc ccggcgacgt gggcgtcttg atgacgatct ccggcgctgg
     6061 agtgctccgg tcgacgcgtg tgctggcaac cgtcgacggc gtcaagtctg tcaggcaact
     6121 cgaggacgaa accgagtagt cagcggtgct ggccgtaccg ggcggcgaag gcgcccgccg
     6181 gaaacacgcc ggcgcccaac accgcctgcc gcaccggcgc cgcgaaccgc tcgagctcag
     6241 ccgcatcgtc gtcggcaagc gttcgccacg gctcggcggc caacctatcg gtgagatcct
     6301 ctatctccgt gcgtaattgg agacccgagg tggtgagcgc gccggaatcg tcgagcagtc
     6361 gtcgctgccg cagccgatcc gtcgcggccg cccacttctc ctcgctccag ccccgcagcc
     6421 gacgggcgaa ctcgacggtg aacccgattt cgcttgcggt gtgggtgatc aacgcctcta
     6481 ggccgttgag ctcgtaggtg atcagcgcgg cgatatgccc gtcgccgcgg tactcgcgca
     6541 gcagagtgat cgcatgccac agccgcgcgt gcggcgatcg cggccatggc agctccgcat
     6601 gacccgcgta aagcggtcga ccatcggcgt tcgggatcgc ttccgccaca cgtcgaagga
     6661 tctcagccgc acgcgacaac tccgccgatt cggtcagcga cgtccccagc acaccaggca
     6721 acgcctgctc cacgatcgcg tagcgcatct ccgtcacggt ctgcggtgac gccatctgcc
     6781 aggcggccgg gatcgacgcg ccgaccagct ccgggttgaa gttgtagaag gtggccgcga
     6841 cgacgcccgc tccgaccgct cccatcggtg ccgaccgcgt agcgaaatac ggtgtcctgc
     6901 ctttcattcc gagtgcaccg aattgcgagg tgacgtctgg caggaaatac gcggtcgaat
     6961 gcaagggatc caggctgcgg gtgagacgac ctgccgagaa cgcatccacg ggcgcgactc
     7021 tacttcgcgg cgggattgga tcggcacgtg gcgacgacac cgatcgccgc cagcgcggcg
     7081 aacataccga aaagccaacg ggccgcggcg agatcaccac ccggcaccat gttgaccacc
     7141 actccggcga gtcctgcacc gaacgcgccg gagatgagct ggacggtgtt gatcgccgcg
     7201 gcggccgcac ccccttcgac cggatcgtcc accgcgccca tgatccaggc cgacaggtgc
     7261 ggccacgcga tcccgacgcc ggtacccgcg acgaacagcg cgaccaccca gaccacgatc
     7321 accggccagg tcggccctcc gtccacagtg agcgcggcca accccaggcc gatcgccatg
     7381 atggcgggtg cgacggccac catccggacg accagacggg cccggttcgc cgatgcgctg
     7441 acgatttcgg acagcgtcca gcccaccgcc agcaccgcac cgaggaagcc ggccgccacc
     7501 ggagtcatgt gagcgaggcg ctggccgaac agcggcacgt acatgtcgac catcgtggcc
     7561 gccatcagca gaccgacgct cacatacatc cacttcagcg ggccgggacc gaaggctgcg
     7621 cggggcaaca ccgtcgcccg agcccgccgg tcgaccagca agaagcagcc gaccagcgcg
     7681 acacccagca caagcagtcc cgcggtgacg gacacggtgc gctgtacacc ggcgaggctg
     7741 acggccaggg ccgctaggcc gagcagcagc agcgaccaga ccggtatccg cgtgagcgtg
     7801 gcgggtgcca cgtcgctctt cgcccgcgac ggcaacgcgc ccggcaccaa cgccgccatc
     7861 gccatcgtcg cgatcgccag tgacccgaaa gcccaacgcc agataccgaa ttgggcaaac
     7921 acgccaccca tcgcaggccc gatgatgacg ctgacccccc acatcgccga caccaacgcg
     7981 gacgcacggg tccacagcca gttcggcagc gccgcgttga tcaccgcgta gccaaggcct
     8041 gccagcattc cgccggccgc gccctggatt gtgcgcgcga cgagcagcag ctccatcgtg
     8101 ggcgcgatcg cgcatcccac ggtgcccgcg ccgaacagcg tcagaacgag caggtatgac
     8161 gcccgcggtc cacgtcgcat gagcagcggg ttgaccgctg tcgcggcgac taccgagccg
     8221 accaggtaca cgctgacgac ccacgcgtag aaccgttgcc cgccgatgtc cgcgatcgtg
     8281 ctgggcagca ggctgatagt gaggaactcg ttggtggcgt acagcgcgac accgccggcc
     8341 agcagcgtcg aggtgcggag ccggtcgcgg cccaacagct cgcgccaact gcccgtcgcc
     8401 acggtcgctt cgctcaccca agccacgcta gagcctcaac cgcagttgag atcaagctat
     8461 ttcagcccgg cagcgtccat cccccgcagc tccttcttca ggtcggcgat ctcgtcgcga
     8521 atacgggcgg ccagctcgaa ctgcaagtcc cgcgccgcgg ccatcatctg cgcggtcagg
     8581 tctttgatca agtcagcaag ctccgcccgc ggcatgttcg acgtgtcgcg accctcgtag
     8641 atcccggcgc tgaccgcccg gcccggctca ccctgcgcac ggcgcccccg cgacacattc
     8701 cggcccgagc cgcccacctc gactcgatca ctgtcgtcgg cctcacggta gacctggtcg
     8761 aggatgtcgg cgatcttctt gcgcagcggc ttcgggtcga tgccacgctc ctcgttgtac
     8821 gcgatctgct tcgcgcgtcg ccgctccgtc tcgtcgatgg cctctttcat cgagtcggtg
     8881 accgtgtcgg cgtacatgtg cacctcgccg gacacgttgc gcgccgcacg gccgatggtc
     8941 tggatcaggc tgcgcgacga gcgcaggaag ccctccttgt cggcgtcgag gatcgccacc
     9001 agcgacacct cgggcagatc caacccctcg cgcaacaggt tgatgccgac gagcacgtcg
     9061 tagtcgccga gccgcagctg tctcagcagt tcgacccggc gcagcgtgtc gacctccgag
     9121 tgcaggtagc ggacccggat gcccatctcg agcaagtagt cggtgaggtc ctcggccatc
     9181 ttcttggtca gcgtggtgac cagcacgcgt tcgtcggcgc cggcgcgttt gcggatctcg
     9241 ccgatcaggt cgtcgatctg gcccttggtg ggcttcacca ccaccttcgg gtccaccagc
     9301 cctgtcgggc ggatcacctg ctcgacgaac tcgccgctgg actggctgag ctcgtagggt
     9361 cccggcgtcg ccgacagata cacggtctgg ccgatccggt cggcgaactc ctcccaggtc
     9421 agcggccggt tgtcgaccgc cgacggcagc cggaacccgt agtcgacgag gttgcgcttg
     9481 cgggagatgt cgccttcgta catgccgccg atctgcggga cggtcacgtg cgactcgtcg
     9541 atgacgagca ggaagtcctc ggggaagtag tcgagcagcg tggccggggc cgaccccgcg
     9601 ggccgggcgt cgatgtgccg cgaatagttc tcgattcccg agcagaaccc gacctggcgc
     9661 atcatttcga cgtcgtagtt ggtgcgcatc cgcagccgct gcgcctcgag cagcttgccc
     9721 tggcgttcca gctcggccag ccgctctgcg agctcctgtt cgatcgtgga aatcgcgtgc
     9781 gccatccgct cggggccggc cacgtagtgg gtggccggga agatccgcag cgagtcgacc
     9841 ttgcgaatca cctcgccggt cagcgggtgc aggtagtaca gcgcttcgat ctcgtcgccg
     9901 aagaactcga tgcgcaccgc cagttcctcg tacgagggga tgatctcgac ggtgtcgccg
     9961 cgcacccgga acgaaccgcg ggtgaaggac aggtcgttgc gggtgtactg gacgtcgacc
    10021 aggagccgca gcagcgcgtc acgcggcacc tccatgccta cctgaacctc gaccgagcgg
    10081 tccaggtagg actgtggggt gcccaggccg tagatgcacg acaccgacgc caccaccacc
    10141 acatcccggc gggacagcag actcgacgtg gccgagtgcc gcaaccgctc gacgtcgtcg
    10201 ttgatcgagc tgtccttctc gatgtaggtg tcggtctgcg cgatgtacgc ctcgggctgg
    10261 tagtagtcgt agtacgacac gaagtactcg acggcgt
//
LOCUS       DASWBF010000420         6643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1157624,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000420 DASWBF010000000
VERSION     DASWBF010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157624"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /gene="zomB"
                     /locus_tag="VF874_15620"
     CDS             complement(<1..1049)
                     /gene="zomB"
                     /locus_tag="VF874_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor control protein ZomB"
                     /protein_id="HEX9177436.1"
                     /translation="MRPSFSDNARRLELPARVSLWVGVLLTAALFGWGAWQRRWIADD
                     GLIVLRTVRNLLAGNGPVFNAGERVEANTSTLWTYLVYLGGLVGGPVRLEYVALVLAL
                     LLSVLGVVFLMLGAARLYASSLLGRRAVLLPAGALVYIAVPPARDFATSGLENCLVLA
                     YLGLLWWMMVCWSQQAALSDLRASGSSPTGRGFLGALAFVAGLSVLVRPELALIGGLA
                     LVMMLIAAQGWRTRVLIVVAGGLLPVGYQIFRMGYYGLLVPGTALAKDASGEKWSQGF
                     VYLANFNAPYALWVPAILLVVLGVLVLAARSRSSGRWGHSPASGRYPHPPEGQGDSQG
                     RVTRLVQSPTAVVVF"
     gene            complement(1027..1941)
                     /locus_tag="VF874_15625"
     CDS             complement(1027..1941)
                     /locus_tag="VF874_15625"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX9177437.1"
                     /translation="MSEEAAPVTRPPDNLIAGIVRAIRPRQWVKNVLVFAAAMAAFGT
                     ATYNYRVVLTNVSIAFVVFCMAASAIYLVNDVRDVEADRQHPTKRFRPIAAGVVPEWL
                     AYTLAVVLGVASLAISWLFVNSDLTIVMAVYLAMQLAYCFGLKHQAVLDICIVSSAYL
                     IRAIAGGAAAHIRLSQWFLLVMAFGSLFMVAGKRYAELQLAERTGAKIRKSLESYTGT
                     YLRFVWTLSATAVVLCYGLFAFQRDGGSGSWWVISMIPFTIAILRYAVDVDGGMAGEP
                     EEIALRDHVLQFLAVALIGTVGAAVIFG"
     gene            complement(1934..2455)
                     /locus_tag="VF874_15630"
     CDS             complement(1934..2455)
                     /locus_tag="VF874_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9177438.1"
                     /translation="MADPSEPQGEVAALVAVQSALARRPGVVTAARALSHFGEHSAGW
                     LAVSAAGALMQPDKRRQWLLAGAGAFTAHAAAVVIKRVVRRPRPQHPAVEVNVGTPSA
                     LSFPSAHATSTTAAAILMAPLTGVPLPVLVVPPMALSRLVLGVHYPSDVLTGVVVGAL
                     VARLVNKIGTRDE"
     gene            complement(2448..4337)
                     /locus_tag="VF874_15635"
     CDS             complement(2448..4337)
                     /locus_tag="VF874_15635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9177439.1"
                     /translation="MTSKAVSLLARVILPRPGEPRDVRKLYIEESSTNARRAHAASRT
                     ALQIGAESEVSFSTYFNAFPASYWRRWSTLTSVVLRAELTGTGRVDVYRTKATGARIF
                     LEGRAFDGDQPGILEIEIDLAPFEDGGWIWFDITTDSAVTLHNAGWYAPVPAPGVANI
                     AVGIPTFNRPGDCVNALRALTSDPLVDNVIGAVIVPDQGTAKVRDHPGFAEAAAPLGN
                     RLSIHDQPNLGGSGGYSRVMYEALKNTDCQQILFMDDDIRVEPDSVLRALAMSRFAKV
                     PTLVGGQMLNLQDPSHLHVMGEIVDRSNFMWTHAPHTEYDHDFARKPLSGKDTDSKLL
                     HRRIDVDYNGWWMCMIPRQVADELGQPLPLFIKWDDADYGLRAAEHGYPTVTMPGTAI
                     WHMAWSDKDDAIDWQAYFHLRNRLVVAAMHWDGDVRGLLRSMLKATLKHLLCLEYSTV
                     AIQNKAIDDFLSGPEHIFSILESALPEVHRIRRNFPDAVVLPSATALPPPSGVVGRVG
                     EPKSAPAKVIRLARGILHNFTKANSAHHERPQLNVSTQDSRWFLLCNVDGVTVTTADG
                     RGVVYRQRDRRKMLSLLAQSLRRHRTLANRFDEMRRVYREALPVLTSKEKWESVLLSP
                     TGHHG"
     gene            complement(4334..5530)
                     /gene="glf"
                     /locus_tag="VF874_15640"
     CDS             complement(4334..5530)
                     /gene="glf"
                     /locus_tag="VF874_15640"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEX9177440.1"
                     /translation="MTKRFDLFVVGSGFFGLTIAERVATQLGKRVVVIERRPHIGGNA
                     YSEPEPQTGIEVHKYGAHLFHTSNTRVWEYVNQFTDFTGYQHRVFAMHNGQAYQFPMG
                     LGLVSQFFGRYFSPDEARALIADQASEIETAEAQNLEEKAISLIGRPLYEAFVKGYTQ
                     KQWQTDPKELPAGNITRLPVRYTFNNRYFNDTYEGLPVHGYTAWLQNMAADERIEVRL
                     DTDWFDVRDELRAASPDAPVVYTGPLDRYFDYAEGRLGWRTLDFDVEVLSDCGDFQGT
                     PVMNYNDLDVPHTRIHEFRHFHPERDYPGDKTVIMREYSRFAEDDDEPYYPINTEADR
                     ALLAAYRAHAKAETGSAKVLFGGRLGTYQYLDMHMAIASALNMYDNVLVPHLRDGAPL
                     TDSDAE"
     gene            5882..>6643
                     /locus_tag="VF874_15645"
     CDS             5882..>6643
                     /locus_tag="VF874_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177441.1"
                     /translation="MTAVTALTASPQPAPEKHDFVQAAMVTDLPGEIVSALQSSLSQF
                     GIVVPNVPSGILGPASTPASSALPSPGGFTATPGSLTAPGLTATAPGLTATPTPGFTA
                     LPTTPPVTSPASVLGELANPGLSPGTAALTAPSVTATPPGLITPLAGIDPAMTSPLGA
                     ASSPTSVQGEVPIGAPLGPDGLAGYPVMGDPLTAMPSTAPASGGLLSELSSAAEQLGA
                     SQAIDLLKGVVVPSIMQAIKSAAPAAEAAPAAAVPA"
BASE COUNT         1151 a         2275 c         2160 g         1057 t
ORIGIN      
        1 atgaacacca ccaccgcggt cgggctctgc acgagccgtg tcacccgccc ctgactgtcc
       61 ccctgccctt cgggcgggtg ggggtacctc ccgctcgcgg gggagtgccc ccagcgcccc
      121 gagctgcggc tgcgcgccgc cagcacgagg acccccagca ccaccaacaa gatggccggc
      181 acccacaacg cgtacggcgc gttgaagttg gccaggtaaa cgaatccctg cgaccacttc
      241 tcacccgagg cgtccttggc gagcgcggtc cccggcacca gcaacccgta gtagcccatc
      301 cggaagatct gatagccgac gggtagcagg cccccggcca ccacgatcag cacccgagtg
      361 cgccacccct gagccgcgat cagcatcatc acaagcgcca ggccaccgat cagcgcgagc
      421 tcgggacgca ccagcacgga caggccggcg acgaatgcca gcgcgcccag gaaaccacgg
      481 ccggtcggcg atgagccgct tgcgcgaaga tcagacagcg cagcctgctg gctccagcac
      541 accatcatcc accagagcag cccgagatag gccagcacca aacagttttc gagtccggag
      601 gtggcgaagt cgcgggccgg gggcaccgcg atgtagacga gcgctcccgc cggcagcagc
      661 actgcgcgac ggccgagcaa gcttgatgcg tagagcctcg cggcacccag catcagaaac
      721 acgacgccta gcaccgacag cagcagcgcc agcacgagcg ccacgtactc cagtcgcaca
      781 gggccgccga cgaggccgcc gaggtacacg agataggtcc acagggttga ggtgttggcc
      841 tccacgcgtt cgccggcgtt gaacaccggg ccgttgcccg ccaacaggtt ccgcacggta
      901 cgcagtacga tgagcccgtc gtcggcgatc cagcgccgct gccaggcgcc ccagccgaac
      961 agcgccgcgg tcagcagcac accgacccag aggctcaccc gcgcgggcag ctcgaggcgg
     1021 cgcgcgttat ccgaaaatga cggccgcacc gactgttccg atcaatgcca ccgcaaggaa
     1081 ctgcagcacg tggtcacgca gcgcgatctc ctccggctcg ccggccatcc cgccgtcgac
     1141 gtccaccgcg tagcgcagga tcgcgatcgt gaacgggatc atcgagatga cccaccacga
     1201 gcccgagccg ccgtcgcgct ggaacgcaaa cagcccgtag cacaacacca ccgcagttgc
     1261 cgacagcgtc cagacaaacc gcagatacgt gccggtgtag ctctccagcg acttgcggat
     1321 cttggcgccg gtacgctcgg ccaactgcag ctccgcgtag cgctttccgg ccaccatgaa
     1381 cagcgacccg aacgccatca ccaacagaaa ccattgcgac aggcgaatgt gcgcggccgc
     1441 accgccggcg atcgcgcgga tcagataggc cgacgagacg atgcagatgt cgagcaccgc
     1501 ctgatgtttc aagccgaagc agtacgccag ctgcattgcc aggtagacgg ccatcacgat
     1561 cgttaggtcc gaattgacga agagccacga gatcgccagc gacgcgacac ccagtaccac
     1621 cgccaacgtg taggccaacc actcgggcac cactccagct gcgatcggcc ggaaccgctt
     1681 cgtcgggtgc tgacggtcgg cctcgacgtc gcgcacgtcg ttcaccagat agatcgccga
     1741 ggcggccata cagaacacga cgaacgcgat cgacacgttc gtcagcacga cgcggtagtt
     1801 gtacgtggca gtgccgaatg ccgccatcgc cgcggcgaac accaggacgt tcttcaccca
     1861 ctgccgcggc cgaatcgcgc ggacgatccc ggcgatcagg ttatccggcg gccgagtgac
     1921 tggtgccgct tcttcactca tcacgtgttc cgatcttgtt gacaagccgc gcgacgagcg
     1981 cgccgacgac caccccggtc agcacgtcgc tggggtaatg cacgccaagt accaagcgag
     2041 acaacgccat cggtggcacc acgagcacag gcaacggcac gccggtgagc ggagccatca
     2101 ggatggccgc cgcggtggtc gaggtggcgt gcgcggacgg gaagctgagc gcgctcggtg
     2161 tgccgacgtt cacctcgaca gcgggatgct ggggccgagg ccggcgcacc acccgcttga
     2221 tcaccaccgc agcggcatgg gcggtgaacg cccccgctcc cgccagcagc cactgccggc
     2281 gcttgtcagg ttgcatcagc gcgcccgcag cggataccgc cagccagccg gcactgtgct
     2341 cgccgaagtg cgacaaggcc cgagcggcgg tgaccacgcc gggacgccgc gcgagcgccg
     2401 actgaactgc caccaacgcg gcgacttcgc cctgcggctc cgatgggtca gccatggtga
     2461 cccgtcggtg acaacagcac cgattcccat ttctctttac tcgtcaacac gggcagcgcc
     2521 tcgcggtaca cgcggcgcat ctcgtcgaag cggttggcca acgtgcggtg ccggcgcaac
     2581 gactgggcta agagcgacaa catctttcgc ctatcacgct gccggtagac aaccccgcga
     2641 ccgtcagcgg tagtgaccgt gacgccgtcg acgttgcata gcaagaacca gcgcgagtcc
     2701 tgcgtcgaca cgttcagctg aggccgctca tgatgcgcgg agttggcttt ggtgaagttg
     2761 tgcagtatcc cgcgggccaa gcgaatgacc ttggcgggtg cgctcttcgg ctcgccgacc
     2821 cggcccacta ctcccgacgg cggcggcagc gcggtcgcag acggcaacac caccgcatcg
     2881 gggaaattcc ggcggatccg gtgtacttcc ggcagcgcgg actccaggat ggagaagatg
     2941 tgctccggcc cggagaggaa gtcgtcgatg gctttgttct ggatggcgac tgtcgaatac
     3001 tcaagacaca gaaggtgttt cagggtggcc ttgagcatgc tgcgcagcag gccgcgaacg
     3061 tcgccgtccc agtgcatggc cgccaccacc agccggttgc gcaggtggaa atacgcctgc
     3121 cagtcgatcg cgtcgtcctt gtcgctccac gccatgtgcc agatcgccgt gcccggcata
     3181 gtgaccgtgg ggtagccgtg ctcggcggcc cgcaggccgt agtcggcgtc gtcccacttg
     3241 atgaacagcg gcagcggctg gcccagttcg tcagccactt ggcgcgggat catgcacatc
     3301 caccagccgt tgtagtcgac gtcgatgcgg cggtgcagca gcttgctgtc ggtgtccttg
     3361 ccgctcaatg gtttccgggc gaagtcgtgg tcgtactcgg tgtgtggcgc gtgggtccac
     3421 atgaagttcg agcggtcgac gatctcaccc atcacgtgca ggtgggaggg atcctgcaag
     3481 ttgagcattt gccctccgac gagggtgggc accttggcga accggctcat cgccagcgcc
     3541 cgcagcaccg agtcgggctc gacgcggatg tcgtcgtcca tgaacaggat ctgttggcag
     3601 tcggtatttt tcagcgcctc gtacatcacg cggctgtagc caccggagcc gccgagattg
     3661 ggctggtcat ggatggataa ccggttgccc agcggcgcag cggcttccgc gaagcccgga
     3721 tggtcgcgca ccttggctgt gccctggtcg ggcacgatga cggcaccgat cacgttgtcc
     3781 accaacgggt ctgaggtcag cgctcgcaac gcgttgacgc agtcgccggg ccggttgaac
     3841 gtcgggatgc cgaccgcgat gttcgcgacg cccggcgccg gcacgggtgc gtaccacccc
     3901 gcgttgtgca gcgtgaccgc actgtcagtg gtgatgtcga accagatcca gccgccgtcc
     3961 tcgaacggcg ccagatcgat ctcgatctcg aggatccccg gctgatcgcc gtcgaacgcc
     4021 ctgccctcca aaaagattcg cgctccggtc gccttggtgc ggtacacatc gacgcggccg
     4081 gttccggtca gctccgcgcg cagcaccacc gatgtcagcg ttgaccagcg ccgccagtag
     4141 ctggcaggga acgcgttgaa atacgtcgag aacgacacct ccgactccgc gccgatctgc
     4201 agcgcggtgc gactggcggc atgtgcgcgc cgggcgttgg tgctcgactc ctcgatgtag
     4261 agcttccgca catcccgtgg ctcgccgggc cgcggcagga tgacgcgggc caagaggctg
     4321 acagctttcg aggtcattcg gcgtcactat cggtcagcgg agcgccgtcg cgtagatggg
     4381 gcaccagcac gttgtcgtac atgttcagcg cactcgcgat cgccatgtgc atgtctaggt
     4441 actggtaggt gcccaggcgt ccgccgaaaa gcactttggc cgaaccggtt tcggccttcg
     4501 cgtgggctcg gtaggcggcc agcagcgcgc ggtcggcctc ggtgttgatc gggtagtacg
     4561 gctcgtcgtc gtcctcggcg aaccgtgagt actcgcgcat gatcaccgtc ttgtcgcccg
     4621 ggtagtcacg ctcggggtgg aagtgacgga actcgtgaat gcgggtgtgc ggcacgtcga
     4681 ggtcgttgta gttcatcacc ggggtgcctt gaaagtcccc gcagtcgctc aatacttcga
     4741 catcgaaatc caatgtccgc caacctaatc ggccttctgc gtaatcgaag tagcggtcca
     4801 gtggtccggt gtaaaccacc ggcgcgtccg ggctggccgc ccgcagctcg tcgcgcacgt
     4861 cgaaccagtc ggtgtccagg cgcacctcga tgcgctcgtc ggcggccatg ttctgcagcc
     4921 atgcggtgta cccgtggacc ggcaggccct cgtaggtgtc gttgaagtag cggttgttga
     4981 acgtgtagcg caccggcagc cgggtgatgt tgcccgcggg cagctccttg gggtcggtct
     5041 gccactgctt ttgggtgtag cccttcacga acgcctcgta cagcggccgg ccgatcagcg
     5101 agatcgcctt ttcctcgagg ttctgcgctt cggcggtctc gatctccgac gcctgatcgg
     5161 cgatcagcgc gcgcgcctcg tccggggaga agtagcggcc gaagaactgc gacaccaggc
     5221 ccaggcccat cgggaactgg tacgcctgtc cgttgtgcat cgcgaacacg cggtgctggt
     5281 agccggtgaa gtcggtgaac tggttgacgt actcccacac ccgcgtgttg gaggtgtgga
     5341 ataggtgcgc cccgtacttg tgcacctcga tcccggtctg cggttctggc tccgagtacg
     5401 cgttgccgcc gatgtggggc cgccgctcga tgaccacgac ccgcttaccg agctgcgtcg
     5461 ccacccgttc agcgatcgtc aggccgaaga aaccggagcc gacgacgaag aggtcgaagc
     5521 gctttgtcat cggcagctag ggtatccgag ctaggccttc ccccgtcgct gcgctcgccc
     5581 cggggcgccg ccgacccgtt ttcgccaggc tggcggctgc cgagcgggtc gaaaggtcgc
     5641 gattgcctca cgattccgtc tcgccactct ggtctcatga ttcacgtcag taccatcgga
     5701 catgttggta ttggcgggtc ggcgaacggc cgtcgaccgc gggtgccggc accagttgca
     5761 cctactacgc agacgattcg ttgtccagat tgaggagact tccgtgccga atagacgtcg
     5821 acgcaggctc tcgacagcca tgagcgcggt cgccgccgtg gccgtcgcaa gtccattcgc
     5881 agtgaccgct gtaaccgcat tgaccgcaag tcctcagcca gcgcccgaga agcacgactt
     5941 cgtgcaggcc gcgatggtga ccgacctgcc cggcgagatc gtgtccgcgc tgcagtcgag
     6001 cctgtcgcag ttcggcatcg tggtgcccaa cgttccgtcc ggcatcctcg ggccggccag
     6061 cacgccggcg agttccgcgc tgccgtcccc cggcggcttc accgccacgc cgggcagcct
     6121 gacggcgccg ggcctgaccg cgacggcacc gggcctgacg gcgacaccca cgccgggatt
     6181 caccgcgctg cccacgacgc cgccggtcac gtcgccggcg tcggtgctcg gcgaactggc
     6241 caatcccggg ttgtcccccg gcacggcagc cctgaccgcg ccgagtgtga cggcgacgcc
     6301 gcccggcctg atcacgccgc tggccgggat cgatccggcg atgaccagcc ctctcggtgc
     6361 ggcgtcgagc ccgaccagcg tgcagggcga ggtgccgatc ggcgcgccgc tgggcccgga
     6421 cggcctcgcc ggctacccgg tcatggggga tccgctgacg gcaatgccgt cgaccgctcc
     6481 agccagcggc ggactgttgt ccgaattgtc cagtgctgcg gagcagctcg gggccagcca
     6541 ggcgatcgac ctgctcaaag gcgtcgtggt gccgtccatc atgcaggcga tcaagtcagc
     6601 ggcacccgct gctgaggcgg cacctgccgc ggcggtgccg gcg
//
LOCUS       DASWBF010000421         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_434309,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000421 DASWBF010000000
VERSION     DASWBF010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434309"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF874_15650"
     CDS             <1..337
                     /locus_tag="VF874_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX9177442.1"
                     /translation="KPVLTALFIRAGAVGGMLTPALSTGVATGAFVALAVNHLGGEAS
                     VPTLGLIGGAAVLGITQHAPVFAAVFTAELTHPPLEVWAMLLFAAVGAHLVRKAVTGR
                     QAAKVPAEH"
     gene            complement(347..1234)
                     /locus_tag="VF874_15655"
     CDS             complement(347..1234)
                     /locus_tag="VF874_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177443.1"
                     /translation="MKRGPELARRFFNRFEPVHGLTYFAPEVRAALDGLGYRGFWMGY
                     FAARSAPLGQVPPEVVTAVFYNFAPERVAKALTGAWAVAGPEAALRARHESAVAALRR
                     YGVTDDDSLQTAARLAGTAAKHAGADGRPLFAANRALPWPDKPVLALWHTCTLLREHR
                     GDGHVAVLTAEGVSGRESNVLHAAAGKVPRDYIMRARDYDDVSWQQHERALAERGLLD
                     DAGALTVAGAKLKEHIESSTDRLALSALDALDDDDVEALFQALTPITRLVIAGGDIPA
                     TTPMGLGRDDLDDGSARLT"
     gene            1291..1791
                     /locus_tag="VF874_15660"
     CDS             1291..1791
                     /locus_tag="VF874_15660"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX9177444.1"
                     /translation="MSPIVVMGVSGSGKSTVGAALAQRFGVPFADADDFHPPANIAKM
                     TAGVALDDDDRHPWLDAIGRWLADRCDGGGVMSCSALKRKYRDQLRSHCAQTEFVHLS
                     GSPEVIAARQASRPGHFMPASLMASQFATLELLQPDERGVTLDVDHDIDSIVGAYLAY
                     AQEKES"
     gene            1791..3209
                     /locus_tag="VF874_15665"
     CDS             1791..3209
                     /locus_tag="VF874_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEX9177445.1"
                     /translation="MTLTLLAADQPQPVAAWWQLILAALAGIALIVVLITVAKVHPFL
                     ALIFGSLMVGSVAGENPEKVLESFAKGFGTTAAGVGILIALGAMFAKLLADSGGADEI
                     VDTIVGHASPRALPWAMALVGAIIGLPMFFEIGLVLLMPVIYLVARRSQQSLITVGIP
                     ALAGLSAMHGLVPPHPGPLVAIQNLNADLGITLALGVAVAIPTVIVAGPLFGKLAGRW
                     VVLEAPDRFSTEGSGPSPADEVRRDEPDRQWRRPSFGVTLASVLLPVVLMMGKALTNI
                     FIDDESQLVRVVFNVLGTPLVALLIAVVVAMFTFGVGIGMSRNEVAKCVESGLPPVAG
                     IILIVAAGGGFKQILVDTGIGTMLADWAKGAHISVLLLAWLIAVLIRLATGSATVATV
                     TASSLMLGLVEGMSSGQVALVVLAVGAGSLFFSHVNDAGFWLVKEYFGMTVGQTIKTW
                     SVMETVLSVVGLVLVLVLGLVI"
     gene            complement(3219..3995)
                     /locus_tag="VF874_15670"
     CDS             complement(3219..3995)
                     /locus_tag="VF874_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9177446.1"
                     /translation="MLVRRVARPLLSAVFIGQGIESLRRPEQAAETARPTAEGLQKLP
                     PSAASKVPQDPMTLARVTAGVQIGGGLLLATGKLPRLASAALACTVVPGSLGGHLFWT
                     ETDPQAKAQQRRDFLTDLSLLGGLMIASVDTEGKPSLGWRGRRAAKRASEAVSAALPV
                     GATSSGALLGTVGEKVSHGLQTGAERGRELADVAGERVSPLLDVARERGADWADTARE
                     RGKKAAKKAQKRADKVSKQTQKRAEKATKQAQKRFNEQFA"
BASE COUNT          692 a         1566 c         1596 g          855 t
ORIGIN      
        1 caagccggtg cttaccgcgt tgttcatccg cgccggcgcg gtcggcggca tgctcacacc
       61 cgcgttgtcg accggcgttg cgaccggcgc attcgtggcg ctggccgtca accacctggg
      121 cggagaggcc agcgtgccca ccctggggct catcggcggc gcggcggtgc tcggcatcac
      181 ccagcacgcg ccggtgttcg ccgcggtgtt caccgccgaa ctcactcatc cgccgctgga
      241 ggtgtgggcc atgctgctgt tcgccgcggt gggggcccat cttgtgcgta aggccgtcac
      301 gggccgccag gctgcgaagg tgcccgccga gcattgagcc acgtcttcag gtcaatcgcg
      361 cgctgccgtc gtcgagatcg tcgcggccca gtcccatcgg cgtcgtcgcc gggatgtctc
      421 caccggcgat gacgagccgt gtgatcgggg tgagcgcctg aaatagcgcc tcgacgtcgt
      481 cgtcgtccag cgcgtcgagc gcggacagtg ccagccggtc ggtgctcgac tcgatgtgct
      541 ccttgagctt cgcgcccgcc accgtcaacg ccccggcgtc gtcgagcagc ccacgttcgg
      601 ccagagctcg ttcgtgctgt tgccatgaca cgtcgtcgta gtcgcgggcg cgcatgatgt
      661 agtcgcgcgg caccttgccg gcggcggcgt gcagcacgtt ggattcgcgg cccgagacgc
      721 cctccgcggt cagcaccgcg acatggccgt ctccgcgatg ctcgcgcaga agagtgcagg
      781 tgtgccacag cgcgagcacc ggcttgtcgg gccagggcag cgcccggttc gccgcgaaca
      841 acggacggcc atctgcccct gcgtgtttcg cggccgtacc ggccaggcgg gcggcagtct
      901 gcaggctgtc gtcgtcggtg acgccgtagc ggcgcagcgc ggccaccgcc gattcatgcc
      961 gtgcccgcaa cgccgcctcg ggaccggcga cggcccacgc gcccgtcagc gccttggcga
     1021 cccgttccgg tgcgaagttg tagaacaccg cggtgacgac ctcgggcgga acctggccca
     1081 gcggcgccga ccgcgcggcg aaatagccca tccaaaatcc tcgatagcca aggccgtcca
     1141 gcgcggcgcg cacctcgggc gcgaagtaag tcaatccatg gaccggctcg aagcggttga
     1201 agaagcgtcg tgcgagttcg ggtcctcgtt tcacgttcca gagttaacct caacgtgcat
     1261 acacgtgcag aaacggggta cgcgccaacc atgtcgccga tcgtcgtgat gggcgtgtcc
     1321 ggctcgggca agtcaacggt gggtgccgcg ctggcgcagc gcttcggcgt gccgttcgcc
     1381 gatgccgacg acttccaccc gccggccaac atcgcgaaga tgaccgcggg cgttgctctc
     1441 gacgacgacg accggcatcc gtggctcgac gcgatcggcc gctggttggc tgaccgctgc
     1501 gacggcggcg gggtgatgag ttgctcggcg ctgaagcgca agtaccgcga tcagcttcga
     1561 agtcattgtg cccaaaccga attcgtgcat ctgagcggct cacccgaggt catcgccgcg
     1621 cggcaggcaa gcaggcccgg gcatttcatg ccggcgtcgc tgatggcgtc acagttcgcg
     1681 acgctcgaac tgctgcaacc ggacgagcgc ggcgtgaccc tcgacgtcga tcacgacatc
     1741 gactcgatcg tcggcgccta tctggcgtac gcgcaggaga aggagagctg atgacgctga
     1801 cgttgcttgc cgccgatcag cctcagccgg tggccgcgtg gtggcagttg attctggcgg
     1861 cgctggcggg catcgcgttg atcgtcgtgt tgatcacggt cgccaaggtg cacccgttcc
     1921 tcgcgctgat cttcggcagc ctgatggtcg gcagcgtggc gggggagaac cccgagaagg
     1981 tgctggagtc gttcgccaag ggattcggca ccaccgcggc cggcgtggga attctgatcg
     2041 cactcggcgc gatgttcgcg aaacttctcg ccgactccgg cggcgccgac gagatcgtcg
     2101 acacgatcgt cggtcacgcg tcgccgcggg cgctgccgtg ggcgatggca ctggtcggcg
     2161 cgatcatcgg gcttccgatg ttcttcgaaa tcggcctggt ccttctgatg ccggtgatct
     2221 acctggtcgc gcggcgctcg cagcagtcgc tgatcaccgt cggcattccg gcgctggcgg
     2281 ggttatcggc gatgcacgga ctggtgccgc cgcaccccgg gccgctggtg gcgatccaga
     2341 acctgaacgc tgacctcggc atcacgctag cgctcggcgt ggcggtggcg atcccgacgg
     2401 tgatcgtcgc gggtccgctg tttggcaagc tggccggccg ctgggtcgtg cttgaggcgc
     2461 ccgacaggtt ctccaccgaa ggatccggcc ccagccctgc cgacgaggtc cgccgcgacg
     2521 aaccggacag gcagtggcgg cggccgtctt tcggcgtcac gttggccagc gtgctgctgc
     2581 cggtggtgtt gatgatgggc aaggcgctca ccaacatctt catcgacgac gaaagccagc
     2641 ttgtccgcgt cgttttcaac gtcctcggca cgccgttggt ggcgctgctg atcgccgtgg
     2701 tggtcgcgat gttcaccttc ggtgtgggca tcggtatgtc acgcaacgag gtggcgaagt
     2761 gcgttgagtc cggtcttccg ccggtcgcgg gcatcatctt gatcgtcgcc gccggcggcg
     2821 ggttcaagca gatcctcgtg gacaccggaa tcggcaccat gctcgccgac tgggcgaagg
     2881 gcgctcacat ctcggtgctg ctgctggcat ggctgatcgc ggtgctgatc cggcttgcca
     2941 ccggttccgc aacggtcgcc acggtcaccg cgtcgtcgtt gatgctcggc ctggtcgagg
     3001 gcatgagcag cggccaggtt gcgctggtcg tgctcgccgt cggtgcgggc tcgctgttct
     3061 tctcccacgt caacgacgcc gggttctggc tcgtgaagga gtacttcggc atgacggtcg
     3121 ggcagaccat caagacctgg tccgtgatgg agaccgtgct gtcggtggtg gggttggtct
     3181 tggtgctggt gctaggtctc gtgatttgac ccgccgactc aggcgaattg ctcgttgaac
     3241 cgcttctgcg cctgcttggt cgctttctcg gcgcgctttt gggtctgctt gctcaccttg
     3301 tcggcccgct tctgggcctt cttggcggcc ttcttgccgc gctccctggc ggtgtcggcc
     3361 cagtcggcac cgcgttcgcg cgcgacgtcg agcagcggcg agacgcgctc gccggccacg
     3421 tcggcgagct cccgcccgcg ttcggcaccg gtttgcagcc cgtggctgac tttctcgccg
     3481 acggtgccga gcagtgcgcc gctggacgtt gctcccacag gcagcgcagc ggacacggcc
     3541 tcggacgccc gcttggccgc acgacgaccg cgccacccga gcgacggctt gccctcggtg
     3601 tccactgacg cgatcatcag cccgccgagc aggctcaggt cggtgaggaa atcgcgacgc
     3661 tgctgcgcct tggcttgtgg gtcggtctcg gtccagaaca ggtgcccgcc aaggcttccc
     3721 ggcaccacag tgcacgccag cgccgccgac gccaggcgcg gtagcttgcc ggtcgccagc
     3781 agcagaccgc cgccgatctg caccccggcg gtgacacgcg ccaacgtcat ggggtcttgg
     3841 gggaccttgc tcgccgcgga cggaggcagc ttctgcagcc cctccgcggt cggacgcgcg
     3901 gtttcggcgg cttgctcggg ccgccggagc gactcgatcc cttgtccgat gaacactgct
     3961 gatagaagcg gacgcgcaac tctgcgaact agcatgcctc cggatgccct tcctttccgg
     4021 ttcgcaaacc atcacctgaa atgtgatcgc actcgcaccg tcggcggcgt tgccgcctac
     4081 tttggcacgg gttcgggttt agcctcgatt cccggaggcg tctgagtccg tttttcctgt
     4141 aaagccttcg aaagcctggc tgtacaactc ggctggtaga cggtgtatcg ccagcgtatc
     4201 ctgatcgatg gcctcaatcg gattgctaga gcgaagatgg gaatcctatt cgcgttgtat
     4261 cggatagtgg catccgagga tccaaacgac attgttgtaa ttctctgtct cacagctaga
     4321 ttgctcggcg cgtcgcgctg accgagctaa aaacagctct accataaata catgcggctg
     4381 cggaaatcca attgagggca ggggcggagt acttcaccgc gccgagcggt gcgggacaac
     4441 gccgctacga ggcactgcgc gcctacttca tggaggaggc caccgccgcc gaggtagccg
     4501 acaggttcgg gtactcccac ctcggtgcat cagatggcca ccctgctgcg caccggcaag
     4561 ctcgacccct tcaccgagac caagccgggc ccgaaagggc cgcacaaagc caccggcgac
     4621 gtccgcgaca aggtactggc gttgcgggcc gaagaccgca gcatcggcga gatcgccgag
     4681 gcgctggccg ccgcggggac gccgatctc
//
LOCUS       DASWBF010000422         8491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_700893,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000422 DASWBF010000000
VERSION     DASWBF010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700893"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2557)
                     /locus_tag="VF874_15675"
     CDS             complement(<1..2557)
                     /locus_tag="VF874_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HEX9177447.1"
                     /translation="MTAIFANVEREPDRADELRRDVNVARWVATIAGLIGFVLSIATP
                     LLPVEQTTATLNWPQQGEVSNVTSPLISQAPVSMAVTVPCDVIRGLPPQGGLVLATAP
                     KKGKEATLNALFVNVTTQRVDITDRNVVVASVPRAQVVSPACDRIDITSSDKGTFATF
                     VGLTDSTGKPLRSGFEDPNLRPGIVGVFTDLTGPAPPGLSLSAVIDTRFSTTPTVLKL
                     TAMIVAIVATVVALLALWRLDRVDGRRMHRLIPQRWRFFTTTDVVVVGGFLLWHIIGA
                     NSSDDGYILQMARVADRAGYMSNYFRWFGSPEDPFGWYYNVLALMTHISDTSIWMRLP
                     DLICGLVCWLLLSREVLPRFGPAVASSKAAMWAAGIVLLAAWMPFNNGLRPEGQIATG
                     ALITYVLIERAIISGRLTPAALAVVSAAFTLGIQPTGIIAVAALLAGGRPLLRILVRR
                     HRVIGTWPLVAPLLAAGFVVLTIIFADQTLATVLEATRIRTAIGPNQDWYTENLRYYY
                     LILPTVDGSLSRRFGFLITALSLFASMFIMLRRKRVPGLARGPVWRLMGVIFLTMFFL
                     MFTPTKWVHHFGLFAAVGAAMAAVATVLVSPSVLRWSRNRMAFVSAVFFLLAMCFAST
                     NGWWYVSSYGIPFNNAMPKIGGITISTMLFALFAVTALYTLWLHFVSRDHGEGRIARV
                     LTAAPIPAAASFMVVVFVGSMLLGVVRQYPTYSNAAANLRVFVGGCGLADDVLVEPDP
                     NNGFLKPVPGDYGPLGPLGGGKPVGFTPNGVPEHIVAEALHMNLPQPGTDYDWDAPTK
                     LAQPGINRSTVPLPYGLDPARTPVAGSWVDGPQQQSRLTSAWYELPPPDDGHPL"
     gene            complement(2554..5874)
                     /locus_tag="VF874_15680"
     CDS             complement(2554..5874)
                     /locus_tag="VF874_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HEX9177448.1"
                     /translation="MPGSERSQRAHRIARLIAVVAGLAGIVLCGLVPLLPIKQTTATI
                     LWPHGPGTDGNVSDVTAPVVSGAPLALDVTIPCTAVATLPRSGGLVFSTIPAAGTDAS
                     KYGLFVRANADAVVVAFRDSVAAVAPRTAVAGGACGVLHIWANVGGVGADFVGIPGAA
                     GTLPVEKKPQVAGLFTDLKVPPRDGLAARVDIDTRFITSPTLLKRAVMVLGVICVVAA
                     IVALAALDLRSRRAGDMRLGSGRRPRGSLTRWLRVGFATWLADIGVVGTLLLWHVIGA
                     ISSDDGYNLTIARISGQAGYVANYYRYFGTTEAPFDWYQSLLGQLASVSTAGVWMRLP
                     ATLAGIGCWLILSRCVLPRLGRALAANWVAVWTAGAVFLAAWLPFNNGLRPEPIIAFG
                     TLLTWMLVENAIGTRRLWPAAVAIVVATFAATVAPQGLIALAPLLVGARAVARIIARR
                     RADDGLLAPIAALLASLSLIVVVVFRDQTLATVAESARIKYVVGPTIPWYQDFLRYYF
                     LTVEDSADGTLTRRFAVLVLLLCLFGIIAVVLRRGRLPGVASGPVWRLIGTTAIGLLL
                     LTFTPTKWAVQFGAFAGLAGALGAVTAFTFSRVGLHSRRNLALYVTALLFVLAWATSG
                     LNSWFYVGNYGVPWFDKQPVIAGFPVTVIFLILAIATGVLAGWLHFRLDYTGHTEVTN
                     NRRNRILASTPLLVVAIIMVVLEVGSMAYGAARRYPVYTTARANLDALTSGLSKTSCA
                     MADDVLVEPDTNAGLLQPVSGQKLGKYGPLGGENPVGFTPNGVSDKLEPVEPVTANPG
                     TVNSDGSPNKPSVGIGYAAGTKGGYGPEGVNGSKVALPFGLDPAGTPVMGSYNENTVA
                     AKATSVWYQLPPRTPDRPLVVVAAAGAIWSHDEDGNFNYGQSLQLEWGVQRPDGSFQP
                     LSIVEPIDVASGDVPPQNAWRNLTFPLAWAPPEANVARIVASDPNLSSDQWFGFTPPR
                     VPILETAQQLLGSQTPVLMDIATAANFPCQRPFSEHLGVAELPDYRIMPNYKQTVVSS
                     NMWQSTEDGGPFLITTALLRSETVPSYLRNDWYRDWGSIERYERLVPADQAPNAVVEQ
                     GSVTVRGWTRAGPIRALP"
     gene            complement(5899..>8491)
                     /locus_tag="VF874_15685"
     CDS             complement(5899..>8491)
                     /locus_tag="VF874_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinosyltransferase"
                     /protein_id="HEX9177449.1"
                     /translation="DTRYSSGPTLIKWIAMILGVAMTVVSLIALHVLDTADGMRHRRF
                     LPARWWSATPLDIVVTAVLVWWHFVGANTADDGYILTMARVSENAGYMANYYRWFGTP
                     EAPFGWYYDLLSIWSHVTATSVWVRLPTLVIALMCWWLISREVIPRLGHAVKTNRAAA
                     WTAAGMFLAFWLPLNNGLRPEPIIALGILLTWCSVERAVATNRLLPLAVACIIGALTL
                     FSGPTGIASIGALLVAIGPLRTILHRRSARFGYLALLAPIVAACTVTVILIFRDQTLV
                     GEIEANSLKSAVGPSLSWFDEHIRYERLFMATPDGSVARRFAVLALLVALAVVIAMTL
                     RKGRIPGTAAGPSNRIIGITVISFLAMMFTPTKWTHHFGVFAGLAGSLGALAAVAVTA
                     AVMRSRRNRAVFAALMLFMTALSFASVNGYWYVSNFGVPWSNSFPEFHFGFTTMLLGL
                     TVLVLLAAAWFHFSGRDSDDGADRSWQRIVQSPLAILTWLLVVFEVVSLTAAMIGQYP
                     AWTVGRSNLEALFGKTCGLANDVMVEQDPNAGMLSPIGVPIADGLGAVNSLRFTPNGI
                     PADVSADPVMQQPGSGAFVDNSTGAAANDETGTEGGTTVATGINGSRAQLPFYLDPMR
                     TPVLGSYQSGIQAPAALRSAWYRLPRDEAGPVLVVSAAGRFDPREVQVQWAGDDEKVT
                     GSASFADVGSPPAWRNLRLPMSAIPDDATQIRLTATDDDLAPQHWIVLTPPRIAHLRT
                     LQEVVGSEDPVLLDWLVGMAFPCQRPFGHNDGVVEVPRWRILPDRFGAEANSPVMDNL
                     GGGPLGITELLLRATTVATYLNDDWSRDWGSLQRLTLYYPEVKPARLDLGSATRSGLW
                     SPAPMRH"
BASE COUNT         1524 a         2875 c         2836 g         1256 t
ORIGIN      
        1 ccaacgggtg cccgtcgtcg ggtggcggaa gttcgtacca ggcagaggtc agcctgctct
       61 gctgctgcgg tccgtccacc cagctgccgg ccaccggtgt gcgggccggg tccaatccgt
      121 acggcagtgg gactgtcgac ctgttgatcc ccggctgggc gagctttgtc ggtgcgtccc
      181 agtcgtaatc ggtgcctggc tgcggcaggt tcatgtgaag tgcctcggcc acaatgtgtt
      241 ccggtacacc attcggggtg aacccgaccg gcttgccccc gcccagcgga cccagcggcc
      301 cgtagtcgcc gggcaccggc ttcaagaagc cgttgttggg gtcaggctcg acgagcacgt
      361 catcggccag gccgcacccg ccgacgaata cgcgcaagtt cgcggccgca ttggaatacg
      421 tcggatactg ccgcaccaca ccaagcagca tcgagcccac gaacaccacc accatgaagc
      481 tggccgctgc cgggatcggg gccgcggtca acacccgcgc gatccgacct tccccatgat
      541 cacgcgacac gaagtgaagc cacagggtat acagggcggt gacggcgaac agagcgaaaa
      601 gcattgtgct gattgtaatt ccgccgattt tgggcatcgc attgttaaac gggatcccgt
      661 agctcgacac gtaccaccag ccgttggtgc tggcgaaaca cattgcgagc aagaagaaca
      721 cggccgacac gaacgccatc cggttgcgcg accagcgcag caccgagggc gacaccagga
      781 ccgtggccac cgccgccatc gccgccccga ccgccgcgaa caacccgaag tggtgcaccc
      841 acttggtggg cgtgaacatc aggaagaaca tcgtgaggaa gatgacgccc atcaagcgcc
      901 acaccgggcc gcgggccaat cccggaactc gcttgcgccg caacatgatg aacatggacg
      961 cgaacaacga cagcgcggtg atcaggaacc cgaaccgccg cgacagcgaa ccgtccactg
     1021 tcggcagaat caggtagtag tagcgcagat tctcggtgta ccagtcctgg ttgggtccga
     1081 tcgcggtgcg aatcctcgtg gcctccaaca ccgttgccag ggtttggtcg gcgaatatga
     1141 tcgtcagcac aacgaacccg gccgccagca gcggcgccac cagtggccag gtgccgatca
     1201 cgcggtgccg tcgcaccagg attcgcagca gcgggcggcc accggccaac agcgcggcca
     1261 ccgcgatgat cccggtcggc tggatgccca gcgtgaacgc ggccgagacg accgccagcg
     1321 ccgcgggggt caaccggccg gagatgatcg cacgttcgat gagcacgtag gtgatcaatg
     1381 cgccggtagc aatctggccc tcgggacgca gtccgttgtt gaacggcatc cacgccgcca
     1441 gcagcacgat gccggccgcc cacatcgcgg ccttactgga tgccacagcc gggccgaacc
     1501 ggggcagcac ctcccgggag agcagtagcc agcagaccag cccgcagatc aagtccggca
     1561 accgcatcca gatgctggtg tcgctgatgt gcgtcatcag cgcgagcacg ttgtagtacc
     1621 aaccgaacgg gtcctcggga ctgccgaacc accggaagta gttcgacatg tacccggccc
     1681 ggtccgcgac acgggccatc tgcaggatgt agccgtcgtc ggaggagttg gcgccgatga
     1741 tgtgccataa caagaaaccg ccgaccacga cgacgtcggt tgtggtgaag aatcgccagc
     1801 gctgcgggat cagccggtgc atccgacgcc cgtcgacgcg gtcgaggcgc cagagcgcga
     1861 gcagcgcgac gacggtggcc acgatcgcga cgatcatcgc ggtgagtttc aaaaccgttg
     1921 gcgtggtgga gaaccgggtg tcgatcaccg ccgacagact caacccgggc ggggcaggcc
     1981 cggtcaggtc ggtgaacacc ccgacgatcc ccggccgcag gttcggatcc tcgaacccgc
     2041 tacgcagcgg tttgccggtc gaatcggtca gcccgacgaa cgtggcgaac gtgcccttat
     2101 ctgacgaggt gatgtcgatc cggtcgcagg caggcgaaac cacttgggcg cggggcacac
     2161 tcgccaccac cacattgcgg tccgtgatgt cgacgcgttg ggtggtgacg ttcacgaaca
     2221 gcgcgttgag ggtcgcctcc ttgcctttct ttggcgcggt cgccaggacc agaccaccct
     2281 gaggcggaag cccgcggatc acgtcgcacg gcacggtcac cgccatcgac actggtgcct
     2341 gtgaaatcag cggtgacgtc acgttcgaca cctcgccctg ctgaggccag ttcagcgtcg
     2401 cggtggtctg ctcgaccggc agcagcgggg tggcgatcga cagcacgaac ccgatcaggc
     2461 cagcgatcgt cgcgacccag cgggcgacgt tgacatcgcg ccgcaactcg tcggcgcggt
     2521 ccggttcccg ttcaacgttg gcgaatatgg ctgtcatggc agcgccctga tcggtccggc
     2581 gcgggtccag ccccgcactg tgacgctgcc ctgctcgacg acggcattgg gcgcctggtc
     2641 ggcgggtacc aaccgttcat aacgctcgat cgagccccag tcgcggtacc agtcgttacg
     2701 caggtacgac ggcaccgtct cgctgcgcag cagcgcagtc gttatcaaga acgggccacc
     2761 gtcctcggtg gattgccaca tgttcgacga caccacagtt tgcttgtaat tgggcataat
     2821 ccggtagtcg ggcaattctg cgactccgag gtgctcggag aatggtcgct gacacgggaa
     2881 gttcgccgct gtcgcgatgt ccatcagcac aggggtctgc gaacccagaa gctgctgagc
     2941 ggtttccagg atcggtactc gcggtggggt gaagccgaac cactggtcgg agctcaggtt
     3001 cgggtcagac gcgacgatgc gcgcgacgtt ggcctccggc ggcgcccagg ctagcgggaa
     3061 tgttaggttg cgccatgcgt tctgcggtgg gacgtcgccc gacgcgacgt cgatcggctc
     3121 cacgatcgag agcggctgga agctgccgtc tggccgctgt accccccact ccagctgcag
     3181 cgactgaccg tagttgaaat tgccgtcctc gtcgtgcgac cagatcgcgc cggcggcggc
     3241 caccaccacg agcggccggt ccggcgtccg tggcggcagc tggtaccaca cggaggtggc
     3301 cttggcggca acggtgttct cgttgtagct gcccatcaca ggggtcccgg ccgggtcgag
     3361 tccgaacggc agggccactt tggagccgtt gacaccctcc ggcccgtagc cgcccttcgt
     3421 gcccgccgca tagccgatcc ccacgctcgg cttgttgggc gagccgtcgg agttcacggt
     3481 gcccgggttg gcggtgacgg gctcgacggg ctcgagcttg tcgctgacgc cgttcggggt
     3541 gaatcccact gggttctcgc cgccgagcgg cccgtacttg ccgagcttct ggcccgaaac
     3601 gggttgcagc agcccggcgt tcgtgtcggg ttccaccagc acgtcgtcgg ccatcgcgca
     3661 actggtcttc gacaatccgg aggtcagcgc gtcgaggttg gccctcgccg tggtgtagac
     3721 ggggtagcgt cgcgcggcgc cgtaggccat cgacccgacc tcgagcacga ccatgatgat
     3781 cgcgaccacc aacagaggcg tcgacgcgag gatccggttg cgacggttgt tggtcacttc
     3841 ggtgtgcccg gtgtagtcga ggcggaagtg cagccagccc gccagcaccc ctgtggcgat
     3901 ggccaggatc aagaaaatca ccgtcaccgg gaatccggcg atcactggtt gcttgtcaaa
     3961 ccacgggacc ccgtagttgc cgacgtaaaa ccagctgttc aagcctgagg tcgcccacgc
     4021 caacacgaac agcagcgcgg tgacgtacag cgccaggttg cggcggctgt gcagaccgac
     4081 ccgtgagaag gtgaacgcgg tcaccgcgcc cagcgcgccg gccagtccgg cgaaggcgcc
     4141 gaactggact gcccacttcg tcggtgtgaa cgtcagcagc agcagcccga tcgcggtggt
     4201 gccgatcagg cgccagacgg gcccgctcgc caccccaggt aggcgcccgc gccggagaac
     4261 aaccgcgatt atcccgaaca ggcacagcag caacaccaag actgcgaagc gacgagtcaa
     4321 tgtcccgtcg gcgctgtcct ctacggtcag gaagtagtac cgcaggaaat cctgatacca
     4381 ggggatagtc gggcccacca catattttat gcgcgccgac tcggcgaccg tcgcgagcgt
     4441 ctggtcccgg aacacaacga cgacgatcag cgacagcgag gccaacaacg cggcgatggg
     4501 tgcaagtagg ccgtcgtcgg cgcggcgccg ggcgatgatc cgggcgaccg cgcgtgcgcc
     4561 gaccagcagc ggcgcgagcg cgatcagccc ttgcggtgcg accgtcgcgg cgaacgtcgc
     4621 gacgacgatc gcgaccgcgg ccggccacaa ccgtcgggtg ccgatcgcgt tctcgacgag
     4681 catccaggtc aacagcgtgc cgaacgcgat gatcggctct ggccgcaacc cgttgttgaa
     4741 cggcagccat gcggccagga acaccgcgcc ggccgtccac accgccaccc agttggcggc
     4801 cagcgcgcgc ccgagtcgcg gcagcacgca gcgggagagg atcagccagc agccgatgcc
     4861 ggccagtgtc gcgggcaacc gcatccacac tccggcggtg ctgaccgagg cgagctgccc
     4921 gagcaggctt tggtaccagt cgaacggcgc ctcggtggtc ccgaagtagc ggtagtagtt
     4981 cgcgacgtat ccggcctggc cggagatgcg cgcgatggtc aggttgtagc cgtcgtccga
     5041 cgagatcgcg ccgatgacgt gccagagcag cagcgtgccg acgactccaa tgtcggctaa
     5101 ccacgtggcg aaccccaccc ggagccagcg tgtcaggctg cctcgaggtc gtcgcccgga
     5161 gcccagcctc atgtcgccgg cccggcggct ccggaggtcg agcgcggcca gcgcgacgat
     5221 cgccgcgacc acacagatga cgccgagcac catcacagcc cgcttcaaca gtgtcggcga
     5281 cgtgatgaac cgggtgtcga tgtcgacgcg cgcggccagg ccgtcccgtg gaggcacttt
     5341 caggtcggtg aacaacccgg cgacttgcgg cttcttctcg acgggcagtg tcccggccgc
     5401 gccggggatg ccgacgaagt ccgcgccgac gccgccgacg ttggcccaga tgtgtagcac
     5461 accgcaggcg ccgccggcga cggcggtcct cggcgcgacc gcggccaccg agtcgcggaa
     5521 cgcgacgacg acggcgtcgg cgttggcgcg aacgaacaac ccgtacttgc tcgcgtccgt
     5581 gccggccgcg gggatcgtcg agaacaccag tccgcccgag cggggcagcg tcgcgacggc
     5641 cgtgcagggg atcgtcacgt cgagcgccag cggcgcccca gacaccaccg gcgcggtcac
     5701 gtcggacacg tttccgtctg tccccggccc gtgcggccac aggatcgtgg cggtggtctg
     5761 cttgatgggc agcagcggaa ccaatccgca caacacgatg cccgccagcc cggccaccac
     5821 cgcgatcagc cgcgcgatgc gatgcgcccg ctgggaccgc tcgctgcctg gcacgaggcc
     5881 cgatcgtatg cgacgttcct aatggcgcat cggcgccggg ctccacaggc cgctgcgtgt
     5941 tgccgaaccg agatcgagcc gggccggctt gacctccggg tagtacagcg tgaggcgctg
     6001 cagcgagccc cagtcgcgcg accagtcgtc gttcaggtag gtggcgacgg tggtcgcccg
     6061 caacagcagt tcggtgatcc ccagcgggcc gccgcccagg ttgtccatca ccggcgagtt
     6121 cgcctcggcg ccgaaccggt cgggcaggat tcgccacctc gggacctcga caaccccgtc
     6181 gttgtggccg aacggccgct ggcaggggaa cgccatcccg accaaccagt ccaaaagcac
     6241 tgggtcttcg gaccccacca cttcctgcag cgtgcgcagg tgcgcgattc tcggtggggt
     6301 cagcacgatc cagtgctggg gcgcaaggtc gtcgtcggtg gcggtcagcc ggatctgggt
     6361 ggcgtcgtcg gggattgccg acatcggcag gcgcaggttg cgccaggccg gcggcgatcc
     6421 gacgtcggcg aagctcgcgc tgccggtgac cttctcgtcg tcgcccgccc actgcacctg
     6481 cacctcgcgg ggatcgaatc ggcccgccgc cgagaccacc agcacgggcc cggcctcgtc
     6541 gcgcggcagc cggtaccacg ccgagcgcag cgcggcgggc gcctggattc cggattggta
     6601 gctgcccagt accggcgtgc gcatcgggtc gaggtagaac ggcagctgcg cgcgtgagcc
     6661 gttgattccg gtggcgacgg tggtgccgcc ttcggtgccc gtctcgtcat tggcggccgc
     6721 accggtggag ttgtcgacga acgcgccgct gcccggctgc tgcatgaccg ggtcggcgga
     6781 cacgtcggcc gggatgccgt tcggcgtgaa gcgaagggag ttcaccgcgc cgagaccgtc
     6841 ggcgatcggc accccgatcg ggctcaacat gcccgcgttc gggtcctgtt ccaccatcac
     6901 gtcattggcg agcccgcacg tcttaccgaa cagcgcttcg aggtttgacc gtcccacggt
     6961 ccacgccgga tactgcccga tcatcgcggc cgtcagcgag accacctcga acaccaccag
     7021 cagccaggtc agaattgcca acggcgactg gacaattcgc tgccagcttc tgtcggcacc
     7081 gtcgtcggag tcgcgtccgg agaagtggaa ccacgcggcc gccaacagca ccagcacggt
     7141 gagtcccagc agcatggtgg tgaagccgaa gtggaactcg gggaacgaat ttgaccacgg
     7201 cacaccgaaa ttcgagacgt accagtagcc gttgacgctt gcgaacgaca atgcggtcat
     7261 gaacagcatc agcgcggcga acaccgcgcg attgcgccgt gaccgcatca ccgccgcggt
     7321 gaccgcgacg gcggccaacg caccaagaga tccggccagc ccggcgaaca cgccgaagtg
     7381 gtgagtccac ttggtgggtg tgaacatcat cgcgaggaac gaaatgacgg taatgccgat
     7441 gatgcggttg ctcggaccgg ccgcggtgcc cggaattcgg cccttgcgca acgtcattgc
     7501 gatgaccacc gccagcgcga cgagaagtgc cagcaccgcg aaccggcgcg ccaccgagcc
     7561 gtccggggtc gccatgaaca accgctcgta gcggatgtgc tcgtcgaacc aggagaggct
     7621 cgggccgacc gccgacttca gcgagttggc ctcgatttca ccgaccagcg tctggtcgcg
     7681 gaagatcaga atgaccgtga cggtgcaggc cgcgacgatc ggcgccagca gtgccagata
     7741 tccgaaccgt gccgaccggc ggtgcaggat ggtgcgcagc gggccgatcg ccaccagcag
     7801 agcgccgatc gacgcgatcc cggtgggtcc cgaaaacaat gtcagcgcac cgatgatgca
     7861 ggcgaccgcc aacggcagca gccggttggt ggccaccgcg cgttccaccg agcaccacgt
     7921 cagcaggatg cccagcgcga tgatcggttc aggccgcagc ccgttgttca gcggcagcca
     7981 gaacgccagg aacatgccgg ccgccgtcca cgccgccgcg cggttggtct tcaccgcgtg
     8041 gccgagtcgc gggatcactt cgcggctgat cagccaccag cacatcagcg ctatcaccag
     8101 cgtgggcagc cgcacccaca cgctggtggc ggtgacgtgg gaccagatcg acagcaggtc
     8161 gtagtaccag ccgaacggcg cctccggtgt gccgaaccat cggtagtagt tcgccatgta
     8221 gccggcgttc tccgacaccc gcgccatcgt caggatgtag ccgtcgtcgg cggtgttcgc
     8281 gccgacgaag tgccaccaca ccaggaccgc ggtgaccacg atgtccagcg gcgtggccga
     8341 ccaccagcgg gccggcagga accggcggtg ccgcatcccg tcggcggtgt ccaacacgtg
     8401 cagggcgatc agcgacacca ccgtcatcgc gacgcccagg atcatcgcga tccacttgat
     8461 cagcgtcggc ccgctgctgt accgcgtgtc g
//
LOCUS       DASWBF010000423         9519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_701132,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000423 DASWBF010000000
VERSION     DASWBF010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701132"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VF874_15690"
     CDS             <1..648
                     /locus_tag="VF874_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX9177450.1"
                     /translation="HGNEILADLNPQMPQIRADTERLADLAGVYADASPDLWDALANA
                     VVTARTLNEQQLDLDMALVASIGLGNTGADIFERGGPYLSRGAEDLIPTSQLLDYYSP
                     EIFCMFRNYYEVAPKVWEVLGGYNGYSLRSAGTIVGAGNPYIYPDNLPRVNARGGPEG
                     KPGCWQTITHDLWPMPYLVMDTGYSIAPYNHFELGQPLAVDFVWGRQIGELSINP"
     gene            868..1848
                     /locus_tag="VF874_15695"
     CDS             868..1848
                     /locus_tag="VF874_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX9177451.1"
                     /translation="MRFVYKLSRAWARRLTVAAVMATLLPGLLGVVGSAPAANAFSRP
                     GLPVEYLQVPSASMGRNVLVQFQGGGPHAVYLLDGLRARDDYNGWDLELPVFEWYYQS
                     GLSVVMPVGGMSSFYTDWYQPAVGNGGTWTYKWETFLTQELPAYLAANKSVSTTGNAV
                     VGLSMSGGSALILSAFYPDKFVYAGSLSGFMNPSEGWWPMLIGMAMNDSGGFNATAMW
                     GPSSDPAWQRNDPMLQIPRLVANNTRIWVYCGNGTPSELGGSDLPAKFLEGFTLRTNI
                     TFRDNYLAAGGSNGVFNFPTNGTHSWPYWGSQLQAMKPDIQRVLGATPTA"
     gene            complement(1911..3128)
                     /locus_tag="VF874_15700"
     CDS             complement(1911..3128)
                     /locus_tag="VF874_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEX9177452.1"
                     /translation="MAWERASERVCDLVRQGAEFVLNLPQSWLDEMDEATLSSEYMRT
                     IAADPVLTEATRRSNRANLLHWAAANISHPCAPVPANLSPETLDIARDLVRRGLDESA
                     VDAYRIGHNVAWRFWMQIVFGLTSDPEELREVLDVSARSMSSFVDSTIAGIYRQVEIE
                     REELTRGTHAERREVVALILDGAPITRQRAESRLGYRLDQSHTAAVIWGDEPDTDLAD
                     LDRAAEALMESVDGRRPLSVQSSAATRWVWIPGRDGPDAARVAAALEELSGVRIAIGP
                     TAAGVEGFRRSHLDAVTTQRMVARLRSAQRVASFTDVELVALIMADPERADRFIRDTL
                     GEFESASPELHRTVLTFVHEQCIASRAAARLYTHRNTLLRRLARADEMLPRPLEGTSV
                     HVAVALEALRWRG"
     gene            3255..4757
                     /locus_tag="VF874_15705"
     CDS             3255..4757
                     /locus_tag="VF874_15705"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9177453.1"
                     /translation="MTATSQVRTDHVDVLIVGAGISGIGAAYYLQTRQPAKSFTIIEA
                     RGATGGTWDLFRYPGIRSDSDLHTFGYEFKPWEDEDAIASADKILAYLRETVAENNIE
                     RHIRFHHKVLGAAWSSDDARWRVEVERVDTGERLTISASWLFCAGGYYRYDEGFTPHF
                     EGTEKFRGQIVHPQHWPEDLDYRGKRVVVIGSGATAVTLVPAMADEAGHVTMLQRSPS
                     YIMPVPSKDTIANALKKVLGKRRGYAITRRKNMLKQRAVYRLCQRYPRAARRMIRYIN
                     AKQLPDGYPVDEHFNPVYNPWEQRLCAVPDADMFKAIRDGKASVVTDRIVTFTERGIL
                     LESGAELEADIIITATGLNLQAFGGIALSVDGEPVHLPNTVAYKSMLLSGVPNFAFVF
                     GYTNSSWTLKVGPLCEHFCRLLAHMDAHGYDTCYPEVTNPDMSTRPLLDFDAGYVQRG
                     VNQLPRQGDRGPWRMVMDYRRDVEVLRDGPVTDDNLRFRTSVPAERLVSA"
     gene            4772..5608
                     /locus_tag="VF874_15710"
     CDS             4772..5608
                     /locus_tag="VF874_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9177454.1"
                     /translation="MTSGKVAVITGAGSGIGRALAVGLAERNARLALSDIDETGLDGT
                     VEQVKSLGAEVHAQVLDITDRDAVSAYAAAVHDHYGVVHQIYNNAGIAGGSTVLDTDY
                     AAYERILGVNLWGVINGTKEFLPHLIASGDGHVVNISSLNGIMGQASLSAYCASKFAV
                     RGFTESLSVEMLTAGHPVKVTVVHPGGVKTNIATSALSQAQRAGVATAEQVRRTDSYN
                     EKLLKMPPSRAATIILDGVEAGRSRVLVGNDAKLVDLLVRLLPRWYPLLQARPEHRLL
                     PS"
     gene            complement(5643..6611)
                     /locus_tag="VF874_15715"
     CDS             complement(5643..6611)
                     /locus_tag="VF874_15715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX9177455.1"
                     /translation="MRAAQVTRLDGPDAIEVSDIPEPSGDGVVVDVHAAGVAFPDVLL
                     SRGLYQYKLEPPFVPGGEIAGVVRTAPDGCHVQPGDRVVGLTMISGGMAEVAVLDSQR
                     AFKLPDIVSFEAGAGLLFNDLTVYFALTVRGRLSEGETVLVHGAAGGIGTSTLRIAPV
                     LGASRTIAVVSTEAKKQIATAAGATDVVLADGFKDAVNDLTDGRGVDIVMDPVGGDRF
                     TDSLRSLAPAGRLLVVGFTGGEIPTVKVNRLLLNNIDVVGVGWGAWTLKHPGALTEQW
                     AGLERLLASGRLPAPDPQVYPLEEAAAAVASLENRAAKGKVVLRVR"
     gene            6684..7841
                     /locus_tag="VF874_15720"
                     /pseudo
     CDS             6684..7841
                     /locus_tag="VF874_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            7853..8929
                     /locus_tag="VF874_15725"
     CDS             7853..8929
                     /locus_tag="VF874_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177456.1"
                     /translation="MDFQFTEEQTLLRDTTRSLLSRSYDQESRNKVADTELGWSREVW
                     NQHAEIGILGLGFDDDAGGQIELMVVMTEIGRHLAPEPVVHAALVPGSLIAELGTAGQ
                     RGLLHDIAAGQRLLAFAREGTTSAARQSDSWTLDGRKNPVLAGDSADTLVVSAALPGG
                     GTGVFLVDATAVTRHPYRTFDGRRAAQIDFDNTPAQPLGDASDSSEQIQRAVIRTQSA
                     LCAEAVGAMEEALRLTTEYLKTRKQFGVTLNKFQTLTQRAADMYVSLEMARSMSYYAA
                     MSIADGHVDPLVASRAKLQIGRSGRHVGQEAIQLHGGIGVTAEYPIGHYTARLTAIEQ
                     TLGSSRDHLRLLAGRVGDYDVVTL"
     gene            9104..9519
                     /locus_tag="VF874_15730"
                     /pseudo
     CDS             9104..9519
                     /locus_tag="VF874_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560512.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
BASE COUNT         1710 a         3121 c         3130 g         1558 t
ORIGIN      
        1 cacggcaacg agatcctcgc cgacctgaat ccgcagatgc cgcagatccg tgccgacacc
       61 gagaggctgg ccgacctcgc cggcgtgtac gccgacgcgt cgccagacct gtgggacgcc
      121 ttggccaacg cggtcgtcac agcccgcacc ctgaacgagc agcagctcga cctcgacatg
      181 gcgttggtgg cctcgatcgg tctcggcaac actggtgccg acatcttcga gcgcggcggc
      241 ccatacctga gtcgcggtgc cgaagacctc atccccacct cgcaactgct cgactactac
      301 agccccgaga tcttctgcat gttccgcaat tactacgaag tggcccccaa ggtttgggag
      361 gtgctcggtg gttacaacgg ctactcgctg aggtcggcgg gaaccatcgt gggcgccggt
      421 aacccgtaca tatacccgga caacctgccg cgagtgaacg cccgaggcgg gccagaggga
      481 aagccgggtt gctggcagac catcacccat gatctgtggc ccatgccgta tctggtgatg
      541 gacaccggct acagcattgc tccgtacaac cacttcgaac tcggccaacc gctggcagtc
      601 gatttcgtat gggggcgcca gatcggcgaa ctcagcatca atccgtagta cgctgacgaa
      661 atctattctg ctgcagcata ttccgacgca ggtggcagtc atgcaactcc gtcgtcgagt
      721 cctcgtcgac gtgaaatcgt tagccaactg cgacatcaag atcgggtgta gatcgccggg
      781 acagcgcgtg aaagatggtt cactacccca gaacaacgac gtcgatcgca tgtggtgcgg
      841 catacgaaag atgggatgga agtgaacatg aggttcgtgt acaagctctc gcgtgcctgg
      901 gcgcgccggc tgacggtcgc cgccgtgatg gcgacgttgc tgccgggtct tctgggcgtt
      961 gtcggcagtg cgccggcagc caacgcgttc tcgcgcccgg gtctgccggt ggagtacctg
     1021 caggttccgt cggcgtcgat gggacgaaac gtcttggtgc agttccaggg cggcggtccg
     1081 catgcggtgt atctgctcga cggcctgcgt gcccgcgacg attacaacgg ctgggacctc
     1141 gagctgccgg tgttcgagtg gtactaccag tcggggttgt cggtggtcat gcccgtcggc
     1201 ggcatgtcga gcttctacac cgactggtat cagccggcgg tcggcaacgg cggcacctgg
     1261 acgtacaagt gggagacctt cctcacccag gaactgcccg cctacctggc agccaacaag
     1321 agcgtgtcga cgaccggcaa cgccgtggtc gggttgtcga tgtcgggcgg ctccgcgctg
     1381 attctgtcgg cgttctaccc ggacaagttc gtctacgccg gctcgctgtc cggcttcatg
     1441 aacccgtccg agggctggtg gccgatgctg atcggcatgg cgatgaacga ctccgggggt
     1501 ttcaacgcga cggcgatgtg gggtccgtcc agcgatcccg cgtggcagcg aaacgacccg
     1561 atgttgcaga ttccgcggtt ggtggccaac aacactcgca tctgggtgta ctgcggcaac
     1621 ggcacaccga gcgaactggg cggctccgat ctgccggcca agttcctgga agggttcacg
     1681 ctgcggacca acatcacctt ccgtgacaac tacctggcgg cgggcggctc gaatggcgtg
     1741 ttcaacttcc ccaccaacgg cacccactcg tggccgtact ggggttcaca gttgcaggcc
     1801 atgaagccgg acatccagcg ggtgctgggc gccacaccca ctgcctgacc gtttgcgcca
     1861 cttcggcccc ggcacagcga cgtgccgggg ccgaagtgtc tcagagggtc tcagccgcgc
     1921 cagcgcagcg cttccagtgc caccgccaca tgaacgctgg tgccctccag tgggcgtggc
     1981 aacatctcgt cggcgcgagc aagcctgcgc agcaacgtgt ttcgatgtgt gtacagcctg
     2041 gcggcggccc gggacgcgat gcactgttcg tggacgaacg tcagcaccgt ccggtgcagc
     2101 tccgggctgg ctgactcgaa ttcgccgaga gtgtctctga tgaatcggtc ggctcgctcg
     2161 gggtcggcca tgatcagcgc gacgagttcg acgtcggtga agctggccac ccgctgcgcg
     2221 gatctcagcc gggccaccat gcgctgggtg gtgaccgcgt cgaggtgact gcggcggaat
     2281 ccctcgacgc cggccgcggt ggggccgatc gcgatccgca ccccggagag ctcctccagc
     2341 gcggccgcga cccgagcggc gtcgggacca tcgcggccgg gaatccacac ccagcgggtc
     2401 gcggcgctgg actgaacgct cagcggacgc cggccgtcca ccgattccat caacgcttcc
     2461 gcggcacggt ccaggtcggc cagatcggtg tcgggctcgt cgccccagat gacggctgcc
     2521 gtgtgactct gatcgaggcg atagccaagc cggctctccg ctcgctggcg ggtaatcggc
     2581 gcgccgtcca ggatcagagc gacgacttcc cgacgctcgg cgtgcgtgcc gcgggtgagc
     2641 tcttcgcgtt cgatctcgac ctgccggtag atacctgcga tcgtcgagtc gacgaacgaa
     2701 ctcatcgagc gcgccgacac gtcgagcacc tcgcgcaatt cctcgggatc cgaggtcaac
     2761 ccgaagacga tctgcatcca gaaccgccac gccacgttat gcccgatccg gtaggcgtcc
     2821 acggcagact cgtcgaggcc gcggcgaacc aggtcacggg cgatatcgag cgtctcggga
     2881 ctcagattgg ccggtaccgg tgcgcatgga tgactgatgt tggcggccgc ccagtgcaat
     2941 agattcgcgc ggttgctccg gcgggttgct tcggtcagga ccggatcggc ggcgatggtg
     3001 cgcatgtact ccgacgacag ggtggcctcg tccatctcgt cgagccacga ctgcggcaga
     3061 ttcagaacga actcggcgcc ttgccgcacc aggtcgcaca cccgctcgga cgcgcgttcc
     3121 caggccatgc cgaccaccgt agtcggccag tgcacactgc accaccaatt agtgaaatag
     3181 gtgcgtaatg cccttgtttg actacggtgt tcccgccgac gattggactc gatcaaccag
     3241 tcaaaggagc cagcgtgact gccacctcac aggtccgaac cgaccacgtc gacgtcctga
     3301 tcgtcggcgc aggcatctcc ggaatcggtg ctgcctacta tctgcagacc cggcaacctg
     3361 ccaaatcgtt cacgatcatc gaggcacgcg gcgccaccgg cggcacctgg gatctgttcc
     3421 gctacccggg aatccgctcg gactccgacc ttcacacctt cggctacgag ttcaagccat
     3481 gggaggacga ggacgcgatc gcgagcgccg acaagattct cgcctacctc cgcgagaccg
     3541 tcgccgagaa caacatcgag cgccacatcc ggttccacca caaggtgctc ggcgccgcct
     3601 ggtcgagcga cgacgcccgg tggcgggtcg aggtagagcg cgtcgacacc ggtgaacgtc
     3661 tgacgatcag cgcgagctgg ttgttctgcg ccggcggcta ctaccgctac gacgagggct
     3721 tcaccccgca cttcgagggc accgagaagt tccgcggcca gatcgtgcac cctcagcatt
     3781 ggccggagga tctcgactac cgcggcaagc gtgtggtcgt gatcggcagc ggcgcaaccg
     3841 ctgtcacgct ggttcccgcg atggccgatg aggccgggca cgtgacgatg ctgcagcgct
     3901 caccgagcta catcatgccg gtgccgtcga aggacacgat cgccaacgcg ctgaagaagg
     3961 tactgggcaa gcggcgcggc tacgcgatca cccggcgcaa gaacatgctg aagcagcgcg
     4021 cggtttaccg cctgtgccaa cggtatccgc gggccgcgcg gcggatgatc cgctacatca
     4081 acgccaagca gctgcccgac ggctatcccg tcgacgagca cttcaacccg gtctacaacc
     4141 catgggagca gcggctgtgc gcggtacccg acgccgacat gttcaaagcc atccgcgatg
     4201 gcaaggcctc cgtggtcacc gaccgcatcg tcacgttcac cgagcggggc atcttgctgg
     4261 agtcgggcgc agagctggaa gccgacatca tcatcaccgc aacaggattg aacctgcagg
     4321 ccttcggcgg catcgcgctg agcgtcgacg gcgagccggt gcacctgccg aacacggtgg
     4381 cgtacaagag catgttgctg tcgggtgtgc cgaacttcgc gttcgtgttc ggctacacga
     4441 attcgtcgtg gacgctgaaa gtcggtccgc tgtgcgagca cttctgccgg ctgctcgcgc
     4501 acatggatgc gcacggctac gacacttgct atcccgaggt caccaacccg gacatgtcga
     4561 cgcggccgct gctggacttc gacgccgggt acgtgcagcg cggggtcaac cagctgcccc
     4621 gccaaggtga tcgtggcccg tggcggatgg tgatggacta ccggcgcgac gtcgaggtgc
     4681 tgcgcgacgg accggtcacc gacgacaacc tgcgcttccg cacgtcggtg cctgccgagc
     4741 ggctggtgag cgcatgacat ctggtgagcg catgacatca ggaaaggtcg cggtgatcac
     4801 cggcgcgggg tcgggcatcg ggcgggcact ggctgtcggg ttggccgaac gcaacgcacg
     4861 gctggcgctg tcggacatcg acgagaccgg cctcgacggc accgtggagc aggtgaagtc
     4921 gctcggcgcg gaggtgcacg cgcaggtgct cgacatcacc gatcgcgacg cggtcagcgc
     4981 ctacgccgct gcggtccacg accactacgg ggtggtgcac cagatctaca acaacgcggg
     5041 catcgccggc ggctcgacgg tgctggacac cgactacgcc gcctacgagc gcatcctcgg
     5101 tgtcaacctg tggggagtca tcaacggcac caaggagttt ctgccgcacc tgatcgcctc
     5161 cggcgacggg cacgtcgtga acatctccag cctgaacggg atcatgggac aggcctcgct
     5221 gtccgcctat tgcgcgtcga agttcgcggt gcgcggattc accgaatcgc tgagcgtgga
     5281 gatgctgacg gccgggcacc cggtcaaggt gacggtggtg catcccggcg gggtgaagac
     5341 caacatcgcc acctcagcgt tgtcgcaggc gcagcgggcg ggagtcgcga cggccgagca
     5401 ggtccggcgc accgacagct acaacgagaa gctgctgaaa atgccgccgt ctcgggcggc
     5461 gacgatcatc ctcgacggcg tcgaggccgg caggagcagg gttctcgtcg gcaacgacgc
     5521 gaagctggtg gacctgctgg ttcggctgct gccgcgctgg tacccgctgt tgcaagcgcg
     5581 cccggagcac cgcctactgc cctcttaatc ccgctggatc ttcggctctt cgcgcaagcg
     5641 gctcatcgca cccgcagaac gactttgcct ttggctgcgc gattctccag cgacgccacc
     5701 gccgcggcgg cctcctccag cgggtacacc tgcggatcag gcgccggcag ccggccggag
     5761 gcaagcagcc tctcgagccc cgcccactgc tcggtcagcg cgccggggtg cttcagagtc
     5821 cacgcgcccc agccgacgcc gaccacgtcg atgttgttga gcagcaaccg attcaccttg
     5881 acggtaggga tctcaccgcc ggtgaagccg accaccagca gccgtcccgc cggcgccagg
     5941 gagcgcagtg agtcggtgaa gcgatcgcct ccgacagggt ccatcacgat gtcgacgccg
     6001 cgaccgtccg tcaagtcgtt gaccgcgtcc ttgaacccgt cggccagcac cacatcggtg
     6061 gcaccggccg ccgtcgcgat ctgcttcttg gcctcggtgc tgaccactgc gatcgtccgc
     6121 gacgccccga gcaccggcgc gatccgcagc gtcgacgtgc cgatcccgcc ggccgcgccg
     6181 tgcaccagca ccgtttcgcc ctctgagagc cgaccccgca cggtcagcgc gaaatacacg
     6241 gtgaggtcgt tgaacagcag gcccgcaccg gcttcgaagc tgacgatgtc gggcagcttg
     6301 aacgcccgct gcgagtcgag cacggcgacc tcggccatgc caccgctgat catcgtcagc
     6361 ccgacgaccc ggtccccggg ctgcacgtgg cagccgtccg gggcggtccg cacgacgccc
     6421 gcaatctcgc cgcccggcac gaacggcggc tccagcttgt actggtacag gccgcggctg
     6481 agcagcacgt cggggaacgc gacgcccgcg gcgtgcacgt cgaccaccac gccgtcgccg
     6541 ctgggttcgg ggatgtcgga tacttcgatt gcgtctggac cgtcaagccg agtcacctgt
     6601 gccgcgcgca tgccacctcc gtcatcagcc cgcggcgaga aagcctatcg ttcgaagcga
     6661 cccagaataa acggaggcgt cgtatgcaac tcgcgctgac ggacgacgag gccaagtttc
     6721 gcgatgagct gcgcgagttc tacacgagcg agatcccggc agaaatccgg gaacacgtgc
     6781 gggagggtcg ggagatcgtt cgcgacgata tcgcgaccag ccaccgaatc ctgaatgccc
     6841 acggactggc ggtgccgggc tggcccgtgg agtggggcgg caagaactgg acgccgacac
     6901 agcaccagat ctggctcgac gaaatgcagt tggcgtgcgt gccggaaccg ctgaacttca
     6961 acgcgaagat ggtcgggccg gtgatcgcgg agttcggctc ccagcagctc aaggagcgtt
     7021 tcctgccgcc aaccgccaac atcgacatct ggtggtgcca gggcttctcc gaacccgacg
     7081 ccggctcgga cctcgcgtcg ctgcggacca cggcccagcg cgacggtgac agctacgtcg
     7141 tcaacggcca gaagacgtgg acgacgctgg gccagtatgc ggactggatc ttctgcctgg
     7201 tgcgcacgga cccgcaggtg ccgaagaaac aggccggcat ctcgttcctg ctgatcgacc
     7261 tcgacacccc gggcatcaca atgcgcccga tcaagctgat cggcggcagc gtcgaagtca
     7321 acgaggtgtt cttcgaagac gtgcgggttc ccgccgacca actcgtcggc gaggagaacc
     7381 gaggctggac gtacgcgaag ttccttctgg gcaacgaaag taccggtatc gcgggcgtgg
     7441 gcagaacgaa ggtcaagatc gcggccgtca aacggcacgc cgaggagaag ggcgtgctcg
     7501 acgacccgct gttcgctgcg cgccttgccg aagtcgaaaa cgacttgctc gcactggaac
     7561 tcactcagat gcgagtggtg gcggggtcga cgggcggcaa gccgaacccg gcatcgtcag
     7621 tgctgaagct gaagggtagt cagctgcaac aggcggccac cgaactgctc gtcgagctgg
     7681 ccggcgccga tgccttgccc aatcacgcag gagaccacat cgagtcaccc gggtgggctc
     7741 agcgcgccgc accgacctat ctgaactatc gcaagacgtc gatctacggc ggcagcaacg
     7801 aggttcagcg caacatcatc gcatcgacca ttcttggatt gtgcggcggg acatggactt
     7861 ccaattcacc gaagaacaga ccctgctgcg cgacaccacc cgcagcctgc tgtcgcgcag
     7921 ctacgaccag gagagccgca acaaggtcgc cgacacagaa ctcggctgga gtcgggaagt
     7981 gtggaaccag cacgccgaga tcggaatcct ggggctcggt ttcgacgacg atgcgggcgg
     8041 acagatcgag ctcatggtgg tgatgaccga gatcgggcgg caccttgcgc ccgagcccgt
     8101 cgtccacgcc gcgctggtcc cgggcagcct gatcgccgaa ttgggcactg ccgggcagcg
     8161 tgggctgctc cacgacatcg ccgcgggcca gcggctgctc gcgttcgccc gcgagggcac
     8221 gacaagcgct gcgcggcaaa gtgattcgtg gacactggat ggccgcaaga accccgtgct
     8281 ggccggtgac agcgccgaca ccctggtggt cagcgccgcg ctgcccggcg gcggcaccgg
     8341 ggtgtttctg gtcgacgcga cggccgtcac ccggcacccg taccgcacgt tcgacggtcg
     8401 gcgcgctgcc cagatcgact tcgataacac gccggcgcag ccacttggtg acgcgtccga
     8461 ctcgtcagag cagattcagc gcgcggtgat tcgcacgcag tcggcgctgt gcgcggaggc
     8521 ggtgggtgca atggaagagg cgttgcggct gaccaccgaa tacctgaaga cgcgcaagca
     8581 gtttggcgtg acactgaaca agttccagac gctgacccaa cgcgcggccg acatgtacgt
     8641 gtcgctcgag atggcccgca gcatgagcta ctacgccgcg atgtcaatcg ccgacgggca
     8701 cgtcgatccg ctcgtcgcgt cgcgcgcgaa gctgcagatc ggccgctcgg gtcggcacgt
     8761 cgggcaggag gcgatccagt tgcacggcgg catcggggtg accgcggaat acccgatcgg
     8821 ccattacacg gcgcggctca ccgcgatcga gcagacgctg ggctcgtcac gagatcacct
     8881 gcggttgctc gccggacggg tcggcgatta cgacgtcgtg actctgtagt gcctgcgata
     8941 ggagccgaca cttacacggc tacgaagaca cgaatgataa gtgcaagcgg tggattcttt
     9001 cgccgaagta aatatcccaa attaccggat tcgattcccg ttgctgggac atccagctat
     9061 atttggggct tcctgtggcc aggcggagga gaggtgaacg gagttgggtg aaacacctgt
     9121 cattcccgtt gctgtcatcg ggatggtctg ccggcttccg ggcggcatcg actcccccga
     9181 agggctctgg caggtgttgc tgcggggtga cgaccgtgtc accgagattc cggccgggcg
     9241 gtgggacgct gacgagtact acgaccccga gcctggtacg cccggccggt cggtggcgaa
     9301 atgggatgcg ttcctagacg acagtgccgg cttcgacgct gagttcttcg gtatcagtga
     9361 gcgggaggca accgcgatcc ccagcaccgg ttgttgctgg agacctctgg ggaggccatc
     9421 gtgcatgccg gtctcaaccc ggcgacgatg gccgactcgc ggaccggtgt gttcgtggga
     9481 ttgactcacg ccgactatgc attgctggcc gccgatgcc
//
LOCUS       DASWBF010000424         6928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_106799,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000424 DASWBF010000000
VERSION     DASWBF010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106799"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VF874_15735"
     CDS             complement(<1..623)
                     /locus_tag="VF874_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9177457.1"
                     /translation="MTDDVLAELIASLPDGTVVTDPAITESYRQDRAFDPSAGKPMAV
                     VRPRRTEDVQAVLRWASRHRVAVVPRGAGSGLSGGATALDNGIVLSTERMRDITVDPV
                     TRTAVVQPGLFNAEVKRAVAEHGLWYPPDPSSYEICSIGGNIATNAGGLCCVKYGVTT
                     DYVLGLQVVLADGTAVRLGGPRLKDVAGLSLTKLFIGSEGTLGVITEV"
     gene            complement(644..2224)
                     /locus_tag="VF874_15740"
     CDS             complement(644..2224)
                     /locus_tag="VF874_15740"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HEX9177458.1"
                     /translation="MTTLRQTKPTAGARTGPLLETLAARVTSANAGAYREITNAMTGE
                     PLGRVPHCTPEDVVAAARRAREVQSEWAARPIRERAEVMLRFHDLVLSRQDEVLDIIQ
                     LENGKARRHAFEEVIDVCLTARYYAHTAEEYLTPKRRQGVQLVLTEVWEHHHPKGLVG
                     IISPWNYPLTLGISDALPAIVAGNAVLAKPDDRTPFSALWAVQMLEQAGMPVGLVQQV
                     TGPGSELGTPIIEQSDYLMFTGSTATGRTVAAQAAERLIDCSMELGGKNALLVLDDAD
                     VKKAARGAVRAAFSNAGQLCISVERIYVAAALWDDFVAKFVAATKAMTLSGALDYSAD
                     MGTLINEKQLETVGHHVDDAVSKGASVLAGAQARPDVGPYFYEPTILTGVREGMAAFA
                     DETFGPVVSLYRVETEDEAIAKANDSAYGLNFSVWTSDPDRGRGVAARLQAGTVNVNE
                     AYAAAWASVDAPMGGMKDSGLGRRHGQQGILKYTESQTIAIEKLVPVGAPPWIRADRY
                     AKAMTMGLKALRHVPGVK"
     gene            complement(2323..2766)
                     /locus_tag="VF874_15745"
     CDS             complement(2323..2766)
                     /locus_tag="VF874_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177459.1"
                     /translation="MNINIRANARLAGLAAAACAAAMIAAPTALADPEASDAPPAPDV
                     VVNQAAATDAAPVAAQADAPAEVPHLPSPGNLPPGTTDTPINEGPRTSYLRGIWQAIQ
                     SEDLTWKDGLLMLAQRPMDPGATPPPGVPAGPQQPGAAEPLPPTP"
     gene            complement(2884..4164)
                     /locus_tag="VF874_15750"
     CDS             complement(2884..4164)
                     /locus_tag="VF874_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9177460.1"
                     /translation="MSEPSPRRFALPRRTESTTSRAPVYLLLFATMVAAAGNGISIVA
                     FPWLVLQRNGSALEASIVAGAASLPLLFSTLLAGTAVDFLGRKRISIISDVLSGATVV
                     AIPVLALLLGETAVNVTVLATLAALGALFDPAGITARESMLPEAAARAGWTLDQTNGL
                     YEATFNVAYIVGPGLGGLLIATVGGINTMWVTAATFALSIVAVSLLRLEGAGPPTVDE
                     RPEGVWSGMIEGLRFVWNLKVLRTLALIDLAMTGLYLPMESVLFPKYFTDRSEPAQLG
                     WVLMALSIGGLVGALAYPLLVRRMSRRVTLLIATLTLGVCTAAISVLPPLPVIMLLCA
                     LVGLVYGPIAPIYNYVMQTKSPPQLRGRVVGVMTSLAYAAGPLGFMLAGPLADMFGLQ
                     ITFLALAIPMIVVGLVSLGLPSLYELDRPSTCGP"
     gene            4238..5065
                     /locus_tag="VF874_15755"
     CDS             4238..5065
                     /locus_tag="VF874_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9177461.1"
                     /translation="MRLPHPRLGTLFDSPVPPGSGWPDDPATATTPVTSAPEQVVAMA
                     DSVTSVAQLDAAVSVCRACPRLVDWREEVARVKRKSFAAQPYWGRPVPGWGSERPRVL
                     VVGLAPAAHGGNRTGRVFTGDRSGDVLFAALHRAGLANQALALDAADGLELVDTRIAA
                     AVRCAPPANAPTPAERQTCVPWLDAEWRLVGPHVKVIVALGGFAWRAALQMARVGSPL
                     PRFGHGAEATVGAGVQLLGCYHPSQQNTFTGKLTAAMLDDVFTRARAAAGITGSHGR"
     gene            5075..6112
                     /locus_tag="VF874_15760"
     CDS             5075..6112
                     /locus_tag="VF874_15760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9177462.1"
                     /translation="MRLSVLDLVPVRADQSTADALAATLRLAQAADRLGYTRYWVAEH
                     HNMPSVAATSPPVLIALLGAHTSQLRLGSGGVMLPNHAPLAVAEQFALLEAAYPGRID
                     LGIGRAPGSDPVTSYMLRGPAGRDDRDIDNFPEYLDHVAALMSAHGARVAIPQRQYIL
                     KATPAAVGEPRLWLLGSSAYSAHLAAAKGLPYVFAHHFTGKGTEEALRIYRSEFVPSD
                     VASEPVTFLTVNAVVAPTREEAEALLLPNLQMMARLRTGQPLGPLDLVEDAQAARLSP
                     QEQAVVDAGLARAVVGTPSEAAEQVRALADRFDVDEVMIHPVASARRGTDPATAPGRE
                     ATVELLAKELF"
     gene            complement(6167..6508)
                     /locus_tag="VF874_15765"
                     /pseudo
     CDS             complement(6167..6508)
                     /locus_tag="VF874_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
     gene            complement(6518..>6928)
                     /locus_tag="VF874_15770"
     CDS             complement(6518..>6928)
                     /locus_tag="VF874_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX9177463.1"
                     /translation="IVAGTAPAIRIYEDDDYLAILDIRPFTRGHTLVLPKRHTVDLTD
                     TPPETLADMVAIGQRIGLAARASELGADGNNIAINDGRAAFQSVFHVHLHVLPRRDGD
                     KLSFAKGMLLRRDPDRKETGRILRDALARIDAEQ"
BASE COUNT         1065 a         2383 c         2367 g         1113 t
ORIGIN      
        1 acctcggtga tcaccccgag cgtgccctcg ctgccgatga acaacttcgt gagcgaaagt
       61 cccgcaacgt ctttcagccg cgggccgccg agccgcaccg cggtgccgtc ggccagcacc
      121 acctgcaagc cgagtacgta gtcggtggtg acgccgtact tgacgcagca cagcccgccg
      181 gcgttggtgg cgatgtttcc gccgatgctg cagatctcgt acgacgacgg gtcgggcgga
      241 taccagagcc cgtgttcggc gacagcgcgt ttcacctcgg cgttgaagag ccccggctgc
      301 acgacggccg tgcgggtgac cgggtcgacg gtgatatcgc gcatccgctc ggtggagaga
      361 acgatgccgt tgtccaaagc cgttgcacca cctgataatc cgctccccgc accgcgtggc
      421 accaccgcaa cgcgatgccg gctcgcccac cgcagcacgg cctgcacgtc ctcggtgcgg
      481 cgcggacgca ccaccgccat cggctttccc gccgacggat cgaaagcgcg gtcctggcgg
      541 tatgactcgg tgatcgccgg gtcggtgacg accgtgccgt ccggcagcga ggcaatcagc
      601 tcagcgagga cgtcgtcggt cacgtcccga tcctagggca tccctacttg acgccgggca
      661 cgtggcgcag cgccttcagt cccatggtca tcgccttcgc gtagcggtcc gcgcggatcc
      721 acgggggcgc cccgacgggc acgagtttct cgatcgcgat ggtctgcgac tccgtgtact
      781 tgaggatgcc ctgctgaccg tgccgccggc cgaggccgga gtccttcatg ccgcccatcg
      841 gcgcatccac tgacgcccac gctgcggcgt aggcctcgtt gacgttgacg gtgccggcct
      901 gcagacgcgc agcgacgccc cgtccgcggt ccggatccga cgtccacaca ctgaaattca
      961 gcccgtaggc actgtcgttg gccttcgcga tcgcctcgtc ctcggtctcg acgcggtaca
     1021 gcgacaccac cgggccgaac gtctcgtcgg cgaacgcggc catgccctct cgcacgccgg
     1081 tcaggatcgt cggctcgtag aagtacgggc caacgtcggg ccgcgcctgg gcacccgcga
     1141 gcacgctcgc gcccttgctc accgcgtcgt cgacgtgatg gccgacggtt tcgagctgct
     1201 tctcgttgat cagcgtgccc atgtccgccg aatagtccag tgcgccagac aaagtcatcg
     1261 ccttggtggc ggcgacgaac tttgcgacga agtcgtccca cagcgcagcg gcgacgtaga
     1321 tgcgctcgac cgagatgcac agctggccgg cgttgctgaa cgcggcacgg accgctcctc
     1381 gtgcggcctt cttgacgtcg gcgtcgtcga gcaccagcag cgcgttcttg ccgcccagct
     1441 ccatagagca gtcgatcagc cgctccgcgg cctgcgccgc gacggtgcgc ccggtagcgg
     1501 tggaaccggt gaacatcagg tagtcgctct gttcgatgat cggcgtgccc agctccgatc
     1561 ccgggcccgt cacctgctgc accaggccca ccggcatgcc ggcctgctcc agcatctgca
     1621 cggcccacag cgccgagaac ggggtgcggt cgtcgggctt ggccagcacg gcgttgccgg
     1681 cgacgatcgc cggcagagcg tcgctgatac cgagagtcag cggatagttc cacggcgaga
     1741 tgatgccgac cagtcccttg ggatggtggt gttcccacac ctcggtcagc acgagctgca
     1801 cgccttgccg ccgcttcggt gtcaggtact cctcggcggt gtgcgcgtag tagcgcgccg
     1861 tcaggcacac gtcgatgacc tcctcgaacg cgtgccggcg cgccttgccg ttctccaact
     1921 ggatgatgtc gagcacttcg tcctggcggc tgagcacgag atcgtggaag cgcagcatga
     1981 cctcggcgcg ctcgcgtatc ggccgcgccg cccactccga ctgcacctca cgggctcggc
     2041 gcgccgccgc gacgacgtcc tcgggcgtgc agtgcggcac ccgtccgagc ggctcgccgg
     2101 tcatcgcatt cgtgatctcg cggtaggcgc ctgcgttggc gctggtgacc cgcgccgcga
     2161 gcgtctccaa cagcgggccg gttcgcgcgc ctgcggttgg ctttgtctgg cgaagcgtgg
     2221 tcatcggcgg tctccctcga tcaggcgcgg catggcatgg ctagctccat acttctctcg
     2281 ccgagtagcc gagggaagac cggcggaaac gaatccgggt cgctacggag tcggcggcaa
     2341 aggttcggca gctcctggct gttgcggtcc ggccggaacg ccaggtggag gagttgcgcc
     2401 cggatccatc gggcgctgcg ccaacatcag cagcccgtcc ttccaggtga ggtcctcgct
     2461 ctgtatcgcc tgccagattc cacgcagata agaggtgcga ggaccctcgt taatgggcgt
     2521 atcggtggtg ccgggcggca gattccccgg gctgggcagg tgcgggacct cggctggtgc
     2581 gtcggcctgt gcggcgacgg gtgcggcgtc cgtggcggcc gcctggttga cgacgacgtc
     2641 cggtgctggc ggggcgtccg aggcctccgg atctgccagt gcggtcggcg ccgcaatcat
     2701 tgccgccgca caagcggctg cggcaagccc ggctaaccgg gcgttggctc ggatgttgat
     2761 gttcaccgtc aaacctctcg tgtactgcga agccactgcc taaggcttcc gttcgagcac
     2821 cggattcgtt acagacgtcc tcggcgacgt tgatcaggcg accccaattt tggcggtgga
     2881 gggctacggc ccgcaagtcg acggccggtc cagctcgtac agcgacggca gccccagcga
     2941 caccagcccg acgacaatca tcggaatggc cagcgccaga aacgtgatct gcagtccgaa
     3001 catgtcggcc agcggaccgg ccagcatgaa gcccagcggg ccggccgcat aggccagcga
     3061 cgtcatcaca ccgaccacgc ggccccgtag ttgcggcggc gacttcgtct gcatcacgta
     3121 gttgtagatc ggcgcgatgg gcccgtacac cagtccgacc agggcgcaca gcagcataat
     3181 caccggcagg ggcggcagca ccgagatcgc cgcggtgcac acaccgagcg tgagggtggc
     3241 gatcagtaag gtgacccgcc ggctcattcg ccgcacgagt agtgggtagg cgagcgcgcc
     3301 gacgagcccg ccgatgctca gcgccatcag cacccagccg agctgggcgg gttcgctgcg
     3361 gtcggtgaag tacttcggga acagcacgct ttccatcggg aggtacaagc ccgtcatcgc
     3421 gaggtcgatc aaagcgagtg tgcgcaatac cttgaggttc cagacgaacc gcagtccctc
     3481 gatcatgccc gaccacacgc cttcgggacg ctcgtcgacg gtcggcggcc cggcgccctc
     3541 aaggcgcagc aggctgaccg cgacgatcga caacgcgaac gtcgccgccg tcacccacat
     3601 cgtgttgatg ccaccgaccg tcgcgatcaa cagcccgccg agcccggggc cgacgatgta
     3661 ggcgacgttg aacgtcgcct cgtacaaccc gttcgtttgg tcgagtgtcc agccggcgcg
     3721 ggccgccgcc tcgggaagca tcgactcgcg tgccgtgatc ccagcagggt cgaacaatgc
     3781 gccgagcgca gcgagcgtcg cgagaacggt gacgttcacg gcggtctcgc cgagtagcag
     3841 cgccagcacc ggaatcgcca cgacggtcgc ccccgacagc acgtcggaga tgatcgagat
     3901 gcgcttcctg ccaaggaagt ccacggcggt gccggcgagc agcgtggaga agagcagcgg
     3961 cagcgacgcc gcgccggcca cgatcgacgc ctccagcgcg ctgccgttgc gctgcaagac
     4021 cagccacggg aacgccacga tgctgatgcc attgcccgcc gcggctacca tcgtggcgaa
     4081 caggagcagg tataccggcg cgcgcgacgt ggtggattcg gtccggcggg gcagagcgaa
     4141 gcgacggggg gaaggttctg acatgggaat ctgcccggaa tctagcagtg agacatccgg
     4201 gtcgacgacc gattttccag ccgtgcacag tggaccggtg cgcttgcctc atccgcgact
     4261 cggcacgctc ttcgactcgc cggtgccgcc cggttcgggc tggcccgacg atccggcgac
     4321 cgcgacgacg ccggtcacgt cggcacccga gcaggtggtc gccatggccg attcagtgac
     4381 gtcagtggcg caactcgacg cggcggtctc cgtctgtcgg gcctgcccgc ggctggtgga
     4441 ctggcgagaa gaggttgccc gcgtcaagcg gaaatccttt gccgcgcaac catactgggg
     4501 ccgcccggtg ccgggatggg gatctgagcg gccgcgggtg ctggtggtcg ggctggcacc
     4561 ggcggcccac ggtggtaacc gcaccggccg cgtcttcacg ggcgaccggt ccggagacgt
     4621 gctgttcgcg gcgctgcacc gcgcgggtct ggccaaccag gccctggcgc tcgacgccgc
     4681 ggacggactt gagctcgtcg acacccgcat cgccgcggcc gtgcgctgtg cgccaccggc
     4741 gaacgccccc acgccagccg agcggcagac ctgcgtgccg tggctggacg ccgagtggcg
     4801 gctggtcggc ccgcacgtga aggtgatcgt cgcgctgggc ggcttcgcgt ggcgggccgc
     4861 gttgcagatg gcgcgcgtcg ggtcgccgtt gccgcgcttc ggacacgggg cggaagccac
     4921 cgtcggcgcc ggcgtgcagc tgctcggctg ctaccacccg agccagcaga acacgttcac
     4981 cggcaagctg accgccgcga tgctcgacga cgtgttcacg cgggcgcggg ccgcggcggg
     5041 aattaccggc tcccacgggc gttgacattc accgatgcgc ctttcagtac tcgacctcgt
     5101 cccagtccgc gccgaccagt ccacggcgga cgcgttggcc gcgacgctgc gcttggcgca
     5161 ggccgcggac cgtctcggct acacccgcta ttgggtcgcc gagcaccaca acatgccctc
     5221 ggtcgcggcg accagccctc cggtgctgat cgctttgctc ggcgcacaca cgtcgcagct
     5281 tcggctcggc tccggtggcg tgatgctgcc caaccatgcg ccgcttgccg tcgcggaaca
     5341 gttcgcgctg ctcgaggccg cctaccccgg ccgcatcgac ctcggcattg gacgcgcgcc
     5401 cggctccgac ccggtcacgt cctacatgct gcgcgggccg gccggtcgtg acgatcgcga
     5461 catcgacaac ttcccggagt atctcgacca cgtcgccgcg ctgatgagcg cccacggagc
     5521 ccgggtcgcg atcccgcagc ggcagtacat cctcaaggcc acgccggccg cagtgggcga
     5581 gccgcggctg tggctgctcg ggtcgtctgc gtactcggcg cacctggccg ccgcgaaggg
     5641 gctgccgtat gtgttcgcgc accacttcac cggcaagggc accgaggagg cgctgcgcat
     5701 ctaccgttcg gagttcgtgc ccagcgacgt cgcctcagag ccggtgacgt tcctgaccgt
     5761 caacgccgtg gttgcgccga cgcgcgaaga ggccgaggcg ctgctgctgc cgaacctgca
     5821 gatgatggcg cggctgcgaa ccggtcaacc gctaggcccg ctcgacctcg tcgaggacgc
     5881 ccaggcggcg cggctaagcc cgcaggagca ggcggtcgtc gacgctgggc ttgcgcgcgc
     5941 ggtcgtcggc accccgtcgg aggccgccga gcaggtgcgt gcgctggcgg accgtttcga
     6001 cgtcgacgaa gtgatgatcc atccggttgc gtcggcacgc cgcggaaccg acccggccac
     6061 cgcgccgggc cgcgaggcga cggtggagtt actggcgaag gagctgttct aggtcccagg
     6121 gggcagagcg aagcgacggg ggatctaatc agtggccttc agctcgatga tcaggatcgg
     6181 ccgtgacaat tcgcgccgtc accttgaacc gcttggtgcc gacgttgatg tcgactgtgg
     6241 gaaccttgcg caggttgtgg taccagcccg ggtagcgatc cgacccgcca ttggaggcga
     6301 cgatcaagta ggcatcgcca tcgcgggcgt aagtcagtga attcgtacgc ggtagaccgg
     6361 ttttcgcgcc ggaggtgtgc aacagcagga tgggcggaac tcccgggaac ttgtgcccga
     6421 tgcggccctc ggtcttctga taaacggtgt cgtgcagccg aagcagtcgc ccaccgatct
     6481 ccttctccgc ccaccgcgtg atgttcatgt ctccgtccta ctgctccgcg tcgatgcgcg
     6541 ccagggcgtc acgcaggatg cgtccggttt ccttacggtc ggggtcgcgg cgcagcagca
     6601 ttcccttcgc gaacgacagc ttgtcgccgt cgcgtcgcgg cagcacgtgt aagtggacgt
     6661 ggaacaccga ctggaacgcg gcgcgtccgt cgttgatcgc gatgttgttg ccgtccgcgc
     6721 ccagctccga cgcgcgggcc gcgaggccga tgcgctggcc gatcgcgacc atgtcggcca
     6781 acgtctccgg cggcgtgtcg gtcaggtcca cggtgtgccg ctttggtagg accagcgtgt
     6841 gccctctggt gaacgggcgg atgtcgagga tcgccaggta atcgtcgtct tcatagatcc
     6901 ggatcgcggg cgccgtgccc gcgacgat
//
LOCUS       DASWBF010000425         8103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_51761, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000425 DASWBF010000000
VERSION     DASWBF010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51761"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1597
                     /locus_tag="VF874_15775"
     CDS             80..1597
                     /locus_tag="VF874_15775"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HEX9177464.1"
                     /translation="MAKHRKPKKQAPPSAKSSWLSAPEQSVGAGLAKHAERRRPPALI
                     LAGSTAIAMSAALAFGHATDTTAYSSQIELTAAGPAAIGIGGRGDPTSSNIPAKINGS
                     VVPFGYTYIPVEYPASYFIDDSVDQGVPVLHQAILANAGQDIVVIGYSEGTLVAEEER
                     RALAGSAIDPNKLKFTMIASPNVPNGGIYGRFPHLNLLILSSNGPAQPSQFDTTYYTN
                     EYDPYADFPAYFNPLSLANSLVAVFYVHPDKYYDTITGSTTTTVVPNSAGGTDTYIFI
                     HNADGALPLFQPVRDLSGALGIAFLTEPVLSGEEPVVRLIIDMGYTDRENLDPATPTR
                     FRLITPPGRIIQTVVQMPGAIAQGANNFVSGATSATSFTPFTATPVPTSSSSLRAASE
                     SPVVQQAPVVTKPKDPVPEDNSASQPKPRSQTPPHSINSGYPFSISPVVDNGSKTAPD
                     NTKTRRLGDGRLINAVKDAVTNVFGLKPKHSTQPDTSQSPSPPSTSSQGQQSPAA"
     gene            complement(1608..2120)
                     /locus_tag="VF874_15780"
     CDS             complement(1608..2120)
                     /locus_tag="VF874_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177465.1"
                     /translation="MRQLTRFVATVAAGVMAATSAALLGFGTPVAAADDTTSGTKTVP
                     LSSLLRRCDWSSAPYVPSDNKGSGYALITRTGNTVTAEVHMLAAIPDIWYGVRLVQVP
                     RPGISCAAGDPGVGVGKLFTDGAGTGSVTVSAPVMDGATGAWVSVEGPLGVANQVTGD
                     FRTSDYVAKL"
     gene            complement(2264..2374)
                     /locus_tag="VF874_15785"
                     /pseudo
     CDS             complement(2264..2374)
                     /locus_tag="VF874_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
     gene            2477..3412
                     /locus_tag="VF874_15790"
     CDS             2477..3412
                     /locus_tag="VF874_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9177466.1"
                     /translation="MRPFDLWRHPIGVPRIEKPRSEGRFHLVHGRRLGFAEFGDPAGD
                     VVLWCHGTPGGRRQFPLLGRRAAEKLGLRVVLVERPGSGLSDPHPYTSVAEWATDLAH
                     VADALGAERVGVVGLSGGGPYALACGAVPPLAGRVAAVGVLGGVVPSVGPEASASGAI
                     DLARRFAPLLSALRRPLAMAVSAVLLPVIPFGHLACKAYASTTPEGDQRVLRDPEMEG
                     MFVDDLILATRGRLQAIVDDARLFGRDWGFRLSDVDVPVRWWHGDSDPIVRLEGAQAA
                     IERLPKAELVLRPEESHLGGFATADEVLEFVRAYL"
     gene            3710..4159
                     /locus_tag="VF874_15795"
     CDS             3710..4159
                     /locus_tag="VF874_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9177467.1"
                     /translation="MPDLNDVAELAAANGHLAVVATVRGDGTVQASLVNAGVSTHPVT
                     GKRVLSFVAIGGGAKLANLRARRSVTATFHRGWQWITVEGTADLAGPDDAALGFSQDQ
                     LTTLLRTIFTDAGGTHDDWPTYDETMARERRTAVLITPTRIYSNPGG"
     gene            complement(4156..5355)
                     /locus_tag="VF874_15800"
     CDS             complement(4156..5355)
                     /locus_tag="VF874_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9177468.1"
                     /translation="MTASATTEAHFDPYDVELNADPYPMFRRLREESPLYYNEQYDFY
                     ALSRFADVNKALVDHDTFSSARGAILELIKANIDIPKGVIIFEDPPVHDIHRKLLARM
                     FTPRKINDLEPKIHEFCAQSLDPLIGSGRFDFVADLGAQMPMKVISMLLGIPESDQEA
                     VRDRANNQMRTEAGKPMKAAEQGMVLGEHFATYIDWRAEHPSDDIMTELLNVEFEDET
                     STVRRLTREELLVYLNVVAGAGNETTTRLIGWAGKVLAEHPDQRRELVENPALIPQAI
                     EELLRYEPPAPHVARYVTRDVEYYGQTVPEGSVMMLLIGAACRDHRQFPPDGDVFDIH
                     RQARQHLAFSVGTHFCLGSALARLEGRIALEEILKRFPKWDVDLENAHLSPTSTVRGW
                     ETMPAVV"
     gene            complement(5404..6393)
                     /locus_tag="VF874_15805"
     CDS             complement(5404..6393)
                     /locus_tag="VF874_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9177469.1"
                     /translation="MSVRGAARPTKADVARLANVSTATVSYVLNNVEGQTISPATRAA
                     VRKAAQKLGYRPNLAARNLAVGGSGVVLYVVPRIALGELPMEVGSRLTTALARRGIVL
                     SLQLETDDNQNVVDAMADLNPIAVTSVFPLTGDALAAVTAAGVPQIHLGSAQLRALGD
                     LHLTVGAMRVDHLLARGHSRLAFAYSDIEELRPLGDYWLEGLRVAAINRGLPMIVVGT
                     VSTDGTDAADVVTEWVAQGLTAVCAQSDETAFVVLHGLRQSGLRCPEDLAVIGVDARP
                     LGAVSGPPLTSVAFDAGAIVDVAVAAMMAELGYPVEKEPPSTDVATLIERSST"
     gene            complement(6399..7712)
                     /locus_tag="VF874_15810"
     CDS             complement(6399..7712)
                     /locus_tag="VF874_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9177470.1"
                     /translation="MESGHASTIRKVVTGASIGNAVEWFDFAIYGFLATFIAANFFPS
                     GNETAALLKTFAVFAAAFFMRPLGGFVFGPLGDRIGRQRVLALVILLMSGSTLVIGLL
                     PGYDAIGMAAPLLLLCFRCLQGFSAGGEYGGGAVYLAEFSSDRHRGLVVTFMAWSGVL
                     GFLLGSLTVTVLQAALPSGVMDDYGWRIPFLLAGPLGAVGLYIRLRLGDTPAFTKLRN
                     KSQVAESPLREAFRTEWPAILRVVGLMIVFNVGYYVVFTYLPTYFIKTLHFSNTTAFV
                     SITMASLVALVLILPLAALSDRIGRRPLLIAGALGFAVLAYPLFRLMNSGSAGGAVAA
                     HCALAAIEAVFVAAAVTAGVELFSTRVRYSGFSVGYNIAVAGFGGTTPYVATWLTGRT
                     GNPMAVAFYLMAAAAVSLLTVLRMPETSARPLGVTAPQAVGDVPR"
     gene            complement(7726..7881)
                     /locus_tag="VF874_15815"
     CDS             complement(7726..7881)
                     /locus_tag="VF874_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177471.1"
                     /translation="MQHQLSSAARAFKTQALAAAALPDASMTGTETFRSGCRWYPAYE
                     PDLLPVS"
     gene            complement(7866..>8103)
                     /locus_tag="VF874_15820"
     CDS             complement(7866..>8103)
                     /locus_tag="VF874_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177472.1"
                     /translation="NARPLRILGIRTCSPDSVEWVMKSPRTQAIAVAADVFNTDARLR
                     ETVVRALKRGLTEVTLWGETWPPISTARKPCSTN"
BASE COUNT         1405 a         2735 c         2641 g         1322 t
ORIGIN      
        1 cttttgccgc ataatcgcgt ttcatccggt aacgtccaac ggcgcgccgg gaggggcggc
       61 cggccttaag gagcgtgggg tggcaaagca tcggaagcca aagaagcagg caccgccgtc
      121 ggccaagtcg tcttggctct ccgcgccgga gcaatctgtc ggcgcgggac tggcaaaaca
      181 cgctgagcgt cgtcggccac ccgcgctcat tctggctggt tccaccgcga tcgcgatgtc
      241 ggccgctctc gcgttcggtc acgcgaccga caccaccgcc tacagctccc agatcgaact
      301 gacggctgct ggcccggccg caattggtat cggcggccgg ggcgacccga cctcgtcgaa
      361 catcccggca aaaataaacg gcagcgttgt tccgttcggc tacacctaca ttccggtgga
      421 gtatccggcc tcatatttca tcgacgacag cgtcgatcag ggcgtgccag tgctccacca
      481 ggcgatcttg gccaacgccg ggcaagacat cgtcgtcatc ggctactcgg aaggaacgct
      541 ggtcgccgag gaggagcgcc gggccctggc cggcagcgcg atcgacccaa acaaactcaa
      601 gttcaccatg atcgcctcac cgaacgtgcc caacgggggc atctacgggc gattccccca
      661 cttgaacctg ttgatcctct cttcgaacgg ccccgcgcag ccgtcgcagt tcgacaccac
      721 ctactacacc aacgagtacg acccgtacgc ggacttcccc gcctacttca acccgctgtc
      781 actggcgaat agcctggtgg cggtgttcta cgtccacccg gacaagtact acgacaccat
      841 caccggctcc accaccacga cagtcgtccc gaatagcgca ggcggtacgg acacatacat
      901 cttcatccat aacgcggatg gtgcgctgcc gctgttccag ccggttcggg acctttcggg
      961 tgcgttagga atcgctttcc tcaccgagcc tgtgctgagt ggcgaagagc cggtggtgcg
     1021 actgatcatt gacatggggt acacggatcg cgagaacctc gatcccgcaa caccgactag
     1081 gttccggttg atcaccccgc cagggaggat catccagacc gttgtccaga tgccgggcgc
     1141 gatagcgcag ggcgcgaaca acttcgtcag cggtgcgacg tcggcgacgt cgttcacacc
     1201 gttcacggcg acacctgtac ccacgagctc gtcgtcgttg cgcgccgcgt cagagtcgcc
     1261 ggtggtgcag caggcacccg tcgtcacgaa gccgaaagac cctgtcccag aagacaactc
     1321 ggcgtcgcag ccgaaacccc ggtcgcagac gccgccgcat tcgatcaact ccggctaccc
     1381 gttctccatc agcccggtgg tcgacaacgg cagcaagacc gcgccggaca acaccaagac
     1441 tcgacgtctg ggcgacggaa ggctgatcaa cgcggtcaag gacgcggtca ccaatgtctt
     1501 cggcttgaag ccgaagcact cgacgcagcc ggacacctcg caaagtccgt cgccgccaag
     1561 cacctcgtcg caggggcagc agagcccagc ggcgtgacgc agaaccgcta gagcttcgcc
     1621 acgtaatcgg aggtacggaa atcgccggtg acctggttgg cgacgcccag cggtccctcg
     1681 actgacaccc aggcgccggt cgcgccgtcc atcaccggtg cagagacggt cacgctgccg
     1741 gttccggccc cgtcggtgaa aagcttgccg acgcccacgc caggatcgcc cgccgcgcaa
     1801 ctgatccccg gtcggggcac ctgcaccagc cggaccccgt accagatgtc cggaatcgcg
     1861 gccagcatgt ggacctccgc ggtgaccgtg tttccggtgc gggtaatcag cgcgtatccg
     1921 gaacccttgt tgtccgacgg cacatagggc gcgctgctcc agtcgcaccg tcgcaggagg
     1981 gagctgagcg gcacggtctt cgtacccgac gtcgtgtcgt cggccgcggc gactggagtg
     2041 ccgaatccga gcagcgctgc cgacgttgca gccatgacac ccgctgccac cgtggcgacg
     2101 aaccgtgtga gctgacgcat cgcttggttc cttcctgatc cgcacgcacc ccacccctcg
     2161 caagtattga cgacagccgc gtggctaaac gcgtgaatgg cgaacgagtt gacacagaac
     2221 acccccgggg cccaaggccc cgcggggtgg acgtgatcgt cgtttactgt cgcgcgcgtt
     2281 ggcgctcttc ggccgccttc gctgcggcgc gggccgaatt ggcctcggct tccttcttgc
     2341 cggcgttgcg ctgggcgtcg gccttgtctt tctcctccgt ttgcctcccg tttgccgttg
     2401 ccatccggat tcccacgcgc agccgacacg aaaagtgacc tgcgtcatgt cgaggccgcc
     2461 tacaatcacc ttgccaatga gaccgtttga cctgtggcgt caccccatcg gggtgccacg
     2521 catcgaaaag ccccgctccg aagggcgctt ccatctcgtc cacggccgca ggctgggttt
     2581 cgcggagttc ggcgacccgg ccggcgacgt ggtcctctgg tgccacggca cacccggcgg
     2641 tcgacgccag ttcccgctgc tggggcggcg cgccgccgag aagctcggcc tgcgcgtggt
     2701 actggtcgag cggcccgggt caggcctgtc cgatccgcac ccctacacct cggtggccga
     2761 gtgggccacc gacctggcgc acgtggccga tgccctcggt gccgagcggg taggcgtggt
     2821 cggtttgtcc ggcgggggtc cgtacgcgct ggcctgcgga gcggtgccgc cgcttgccgg
     2881 ccgcgtcgcc gccgtgggcg tccttggcgg cgtggtcccg tccgtcggtc ccgaggcgtc
     2941 ggccagtggc gccatcgact tggcccgccg gtttgcgcca ctgctgtccg ccctgcgccg
     3001 gccgttggcg atggcggtgt ccgcggtgct gctgcccgtc atcccgttcg gccacctcgc
     3061 gtgcaaggcc tacgccagca ccaccccgga aggcgaccag cgggttctcc gcgatcccga
     3121 gatggagggg atgttcgtcg acgacctgat cctggccacc aggggccggt tgcaggcgat
     3181 cgtcgacgac gcccggttgt tcggacgtga ctggggcttt cggttgtccg acgtcgacgt
     3241 gccggtgcga tggtggcacg gggactcgga ccccatcgtg cgcctcgagg gggcgcaggc
     3301 ggcgatcgag cgtctgccga aggccgagct cgtcttacgg cccgaggaga gccacctcgg
     3361 cggcttcgcc accgccgacg aagtcctcga attcgtccgt gcttacctct agcgaagccg
     3421 cggtcaagct gttcgaccgg agcgctcgcg ctggagaacg ccgctgcggt ggccgagccg
     3481 cctgtggcgc agacgaaccc gggcaaaacc gcggccaccg tcgcggcgag tgccagatgc
     3541 aagaaccacg agccacgtac tcgtacgaac ggccatcgct gcccccatcg ggtcgtcctg
     3601 ctgcaacctg agttgcggac gatactgcga caatggtggt aagaatggcg ttttcgacgt
     3661 ggccggcccg gtagtcaacc ggacagccgc gccggggaag gatgtcccga tgcccgatct
     3721 gaatgacgtc gccgaattgg ccgctgctaa cggccatctc gcggtcgtcg cgacggtgcg
     3781 cggcgacggc accgtgcagg cctcacttgt caacgccggt gtcagcactc acccggtcac
     3841 cggcaaacgg gtgctgtcgt tcgtggcgat cggcggcggc gccaagctgg ccaaccttcg
     3901 cgcacggcga tcggtgacgg cgacgttcca ccgggggtgg cagtggatca ccgtcgaggg
     3961 gacagccgat ctcgccggac cggacgatgc tgcgctcggg ttcagccaag atcagctgac
     4021 gactctgctg cgcacgatct tcaccgatgc gggcggcact cacgacgact ggccaaccta
     4081 cgacgagacc atggcccggg aacggcgcac ggccgtcctg atcacgccga cccggatcta
     4141 cagcaacccc ggcggttaga cgaccgcggg catcgtctcc cagccccgaa ccgtggatgt
     4201 cggtgacaga tgggcgtttt cgaggtcgac gtcccatttc gggaagcgtt tgagaatctc
     4261 ttccaacgcg atgcggccct ccagacgagc cagcgccgag cccaggcaga aatgcgtacc
     4321 gacgctgaag gccagatgct gacgcgcctg gcggtggatg tcgaaaacgt cgccgtcggg
     4381 cgggaattgg cggtgatcac ggcaggcggc accgatcagc aacatcatca cgctgccttc
     4441 aggcaccgtc tggccgtagt actcgacgtc gcgggtcacg tagcgcgcca cgtgcggggc
     4501 cggtggctca tagcgcagta attcctcgat tgcttgcgga atgagcgcgg gattctcgac
     4561 gagctcacgc cgctggtcgg ggtgctcggc gagcaccttg ccggcccagc cgatcagccg
     4621 ggtcgtggtc tcgttgcccg cgccggcgac cacgttgaga taaacaagca gctcctctcg
     4681 ggtcagccgc cgcacggtgc tggtttcgtc ctcgaactcg acgttgagca gttcggtcat
     4741 gatgtcgtcc gacggatgct cggcacgcca gtcgatatac gttgcaaagt gctcgccgag
     4801 gaccatgccc tgctcagccg ctttcatcgg tttgccggcc tcggtacgca tctggttgtt
     4861 cgcgcggtca cgaacggctt cctggtcgct ctccgggatg cccaatagca tgctgatcac
     4921 tttcatcggc atctgagcgc caaggtcggc gacgaagtcg aaccgtcccg agccgatgag
     4981 cgggtcgagg ctctgcgcgc agaactcgtg gatcttcggc tccagatcgt tgatcttgcg
     5041 cggggtgaac attcgggcca gcaacttgcg atgaatgtcg tgaacaggcg gatcctcgaa
     5101 gatgatcacg cccttcggga tgtcgatatt cgccttgatc agttcgagga tcgcgcctcg
     5161 agccgagctg aacgtgtcgt ggtcgacaag cgccttgttg acgtcggcga agcggctcag
     5221 cgcgtagaag tcgtactgct cgttgtagta cagcggcgat tcctcccgga gccgccgaaa
     5281 catcggatac gggtccgcgt tcagctcgac gtcgtatgga tcgaagtggg cctcggtggt
     5341 ggcgctggcc gtcatgaagt ctgcctctct gcgggcgaga aggaacttac ccgcgtaagc
     5401 taatcacgtc gaactgcgct ctatcaacgt cgcgacgtcg gtgctcggcg gctccttctc
     5461 gacggggtat ccgagctcgg ccatcatcgc cgccaccgcc acatcgacaa tcgcccccgc
     5521 gtcgaaagcc accgacgtca gcggtgggcc gctgacagcg ccgagggggc gggcatcgac
     5581 gccgatgacg gcgagatcct cagggcaccg caggccggac tgacgcaacc cgtgcagcac
     5641 gacgaacgcg gtctcgtcgc tttgagcgca cacggcggtc agcccttgcg cgacccactc
     5701 ggtgacgacg tccgcagcgt ccgtgccgtc ggtcgaaacg gttccgacga cgatcatggg
     5761 gagtccccga ttgatcgcgg cgacgcgtag gccctcgagc cagtagtccc ccagcggacg
     5821 cagctcctcg atgtcgctgt acgcgaacgc cagccggcta tgcccacgcg cgagcaggtg
     5881 gtccacccgc atcgcgccca ccgtcaggtg cagatcgccg agcgcacgca attgcgcgct
     5941 gccgaggtgg atctgcggca ctccggcggc ggtgacggcc gccagcgcgt cgccggtcag
     6001 cgggaacacg ctggtgaccg cgatcgggtt caagtccgcc atcgcgtcga cgacgttctg
     6061 gttgtcgtct gtctcgagct gcagcgacag tacgatgccg cggcgcgcca gcgcggtcgt
     6121 cagccggctg ccgacctcca tcggcagttc gcccagcgcg atccgcggca cgacgtacag
     6181 cacgacgccg ctaccgccca cggccaggtt tcgggcagcc aggttggggc ggtaccccag
     6241 cttctgggcc gccttccgta ctgccgccct ggtcgccggt gaaatcgtct gtccctcaac
     6301 attattcagc acatagctga ccgtcgcggt cgacacgttg gcgagccggg ccacatcggc
     6361 cttcgtcggc ctggccgctc ccctgacgct cacaggcttc atcgtggcac gtcacccacc
     6421 gcttggggcg ccgtgacacc gagcgggcga gcggaggttt ccggcatgcg caagacggtc
     6481 agcaacgaga ccgccgccgc ggccatcaga tagaacgcga cagccatggg gttaccggtg
     6541 cgaccggtga gccaggtcgc cacgtagggc gtggtgccgc cgaatccggc gacggcgatg
     6601 ttgtagccga cggagaagcc gctgtagcgc acgcgcgtgc tgaacagctc gacccccgcg
     6661 gtgaccgccg ccgccacgaa caccgcctcg atcgctgcca gcgcgcaatg ggcggcgacc
     6721 gccccgcccg cggagccgga gttcatcagc cgaaagagcg gataggccag caccgcgaac
     6781 cccagcgcac cggcgatgag cagcggtcgc cggccgatcc ggtccgacag cgcggcgagc
     6841 ggaaggatga ggaccagcgc caccaggctg gccatcgtta tcgagacgaa cgctgttgtg
     6901 ttggagaaat gcagcgtctt gatgaagtag gtgggcagat acgtgaagac gacgtagtag
     6961 ccgacgttga agacgatcat cagcccgacg acccgcagaa tggcaggcca ctccgtgcgg
     7021 aatgcttcgc gcaacggcga ttcggcgact tgactcttgt tgcgcagctt ggtgaatgcc
     7081 ggggtgtcgc ccaggcgtag ccggatgtag agcccgacgg cgccgagcgg gccggccagt
     7141 aggaacggga tgcgccagcc gtagtcgtcc atgaccccgc tcggaagtgc cgcctgcaga
     7201 acggtgaccg tcaacgaccc gagcaggaac ccgagaacgc ccgaccacgc catgaaggtg
     7261 acgacgagac cgcgatgccg gtccgacgag aactcggcca ggtataccgc acccccgccg
     7321 tactcgccgc ccgctgaaaa cccttgcagg caacggaaac acagcaacag caggggtgcg
     7381 gccatgccga tcgcgtcgta tccgggcagc aatccgatga caagcgtgga tccggacatc
     7441 agcaggatga cgagcgcgag cacgcgctgg cgtccgatcc ggtcgccaag tgggccgaac
     7501 acgaagccgc cgagcgggcg catgaagaag gccgccgcga acaccgcgaa cgtcttgagc
     7561 agggctgccg tctcattgcc cgaggggaag aagttcgccg cgatgaacgt ggcaaggaag
     7621 ccgtagatgg cgaagtcgaa ccactccacc gcgttgccga tggacgcgcc ggtgacgacc
     7681 ttgcggatgg tggatgcgtg gccggattcc acgcggtcag cgtatctagc tgaccggcag
     7741 caggtccggc tcgtatgccg gataccaccg gcacccgctg cggaaggtct cggtcccggt
     7801 catcgacgcg tccggaagtg ccgccgcagc aagggcctgg gtcttgaagg cgcgggcagc
     7861 cgagctcagt tggtgctgca cggcttccgc gccgtggaaa tcggcggcca ggtttcgccc
     7921 cacagcgtga cttcggtgag cccgcgcttc aacgccctga cgacggtttc ccgcaggcgc
     7981 gcgtcggtgt tgaacacatc ggctgccacc gcgatcgctt gtgtgcgcgg cgatttcatc
     8041 acccactcca cgctgtccgg agaacaggtg cggatgccaa gaatacggag cggccgagcg
     8101 ttg
//
LOCUS       DASWBF010000426         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_435741,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000426 DASWBF010000000
VERSION     DASWBF010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435741"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF874_15825"
     CDS             complement(<1..376)
                     /locus_tag="VF874_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9177473.1"
                     /translation="MTPPVIRRWIDIIDNGRSDELETLLSDDAVFCSPAVFTPQEGRA
                     KTLAYLTAAAKLFGNSDFRYVGQWYSDRSAVLEFVADIDGIHVNGVDMIHWNDDEKIV
                     EFKVMLRPLKALQAVIPAMAELL"
     gene            complement(373..936)
                     /gene="pyrE"
                     /locus_tag="VF874_15830"
     CDS             complement(373..936)
                     /gene="pyrE"
                     /locus_tag="VF874_15830"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX9177474.1"
                     /translation="MTTSISWQDAFDVIKRGHEHRDVPFKLASGQTSQHYIDGKHAVD
                     TGARLAVVSRAVVDLAAQRQIEFDAVGGLTMGADALAVGVAMVTGKAWFSVRKEPKSR
                     GLEQFIEGFRLRDHPGTRVLLVEDVVSTGGSVRKAYDKVSEAGAVVAGVIPMVDRGEV
                     AARTFAELGVPYFPLVTYRDLGIEPIK"
     gene            1101..2459
                     /locus_tag="VF874_15835"
                     /pseudo
     CDS             1101..2459
                     /locus_tag="VF874_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729865.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
BASE COUNT          388 a          832 c          829 g          410 t
ORIGIN      
        1 ggagcaactc ggccatcgcc gggatcaccg cctgcaacgc cttcagcggt cgcagcatca
       61 ctttgaactc gacgatcttc tcgtcgtcgt tccagtggat catgtcgacg ccgttgacat
      121 ggatgccgtc gatgtcggcg acgaactcca gcacggccga gcggtcggag taccactgac
      181 cgacatagcg aaagtcgctg ttgccgaaca gctttgcagc ggccgtcagg taggccaggg
      241 tcttggcgcg gccctcctgc ggcgtgaaca ccgctggcga gcagaacacc gcgtcgtcgg
      301 aaagcagcgt ttcgagttcg tcgctccgac cgttgtcgat gatgtcgatc cagcgccgga
      361 tcaccggtgg cgtcatttga tcggctcgat tccgaggtcg cggtaggtga ctaaggggaa
      421 gtacggtacc ccgagctcgg cgaacgtgcg cgcggcgacc tcgccgcggt ccaccatcgg
      481 gatgacgccg gccacgacgg cccccgcctc gctcactttg tcgtacgcct tgcggaccga
      541 tccgccggtg ctgacgacgt cctcgaccag cagcacccgc gtgccggggt ggtcgcgcag
      601 ccggaatccc tcgatgaact gctccaggcc gcgggatttg ggttccttgc gcacggagaa
      661 ccacgccttg ccggtgacca tcgcgacgcc gacggcgagc gcgtcggcac ccattgtcag
      721 accaccgacc gcgtcgaact cgatctgccg ttgggcggcc aggtcgacca ccgcccggct
      781 gacgaccgcg agccgggcgc cggtgtccac ggcgtgcttg ccgtcgatgt agtgctggct
      841 ggtctgcccg ctggcgagct tgaatggcac gtcgcggtgc tcgtggccgc gcttgatgac
      901 gtcgaatgcg tcttgccagg agatcgatgt cgtcaccgcg gtaatgctac ggcggacgcc
      961 tcttcgccga gcgcacggaa gtagtgcgct gggcgaaaaa caagagcgcg ccaggacgtg
     1021 gcgaaaatta ccggcaatcg cggtaagtag ctgcgccaaa gtcggtagcc gtcgcgtccg
     1081 gcatcgctac gctcaatgcc gtggctgaaa cgtcgtacgc accatgcggc gatctgagcc
     1141 tggcgtatca ggtgttcggc gacgggcccg tcgacctcgt ctacgccggg tcgttcgtca
     1201 gccacatcga gctggcctgg accctgcccg aatacgaggc cttcatggag caactcgcga
     1261 cgttctgtcg cgtcctcgtg ttcgacaagg ccggggtcgg gctgtcggac ccggtcccgc
     1321 aagttcgcac gctggatgat cgagcgaccg agatcgaggc cgtcatggac gccgtcggct
     1381 tcggaaaggc cgtcgtcttc ggtatcagcg agggcggccc ggccgccatc gtcttcgcgg
     1441 caacacgccc ggagcgaacg cgggcgttga tcctcaccgg cacgttcgcg ttcaccggag
     1501 ccgggtggga cgacgtcgac cgcgaccctg ccgagatgcg agcgcgcctg gtacccgagc
     1561 tgggtgagga ctacacgccc tcgacgcagc agctcgcccg atggcaggaa ctcagccgcg
     1621 ccgcccgctc ggcgtggggc agcggtgcgg cactcagggg tctgctgccg tcggtgcggt
     1681 cgatacgcca gctcgcaatg ttggagcgca tgagcgccag cccgggcatg gcgcgcgcga
     1741 caatcgaatc gacgttccgg atcgacgtcc ggccgatcct gccgacgatc accgcgccaa
     1801 ccctcgtcat ccatgcccgc gaggacccta ttccggtgca gggcggccgg tacctcgccg
     1861 accatatccc cggcgcgcgg ttgcttgagg tcgacggcgt ggaccacgcg ccctggttca
     1921 ccgatcccga caggatcacc accgcgatcg aggagttcct caccggcagc catgccgcac
     1981 cgtcgcagtc acatcgcgcc ctgcgcacgg tgttgttcac cgacatggtc gcctcgacgg
     2041 aacacgccgc ggcagcgggc gatgagcggt ggcgtgcggt gctgcaccgc tttggtgaga
     2101 tcacggccga cctcacggaa cgattcggtg gcacggtggt gaagggcacc ggcgacggcc
     2161 acctcgccac gttcgacggc ccgacgctgc tattcgctgc gccgaggcct tgcgcgccga
     2221 cgccgagacg ctgggcatcg agatccgcgc tggcatccac accggcgagt gcgaactgct
     2281 ggacgccgac attgggggga tcgccgtgca tatcgcggcg cggattctcg gccaggccgg
     2341 cgccggtgaa atcctcgtct cccgcaccgt gcgtgatctg gtcgtcggct ctggcatggg
     2401 cttcgaggac cgcggcgtcg tcgagctgcg cggtgtgccc ggcacctggc aactcctgg
//
LOCUS       DASWBF010000427         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_561546,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000427 DASWBF010000000
VERSION     DASWBF010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561546"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1708
                     /locus_tag="VF874_15840"
     CDS             <1..1708
                     /locus_tag="VF874_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177475.1"
                     /translation="GALVLPRAGMQSVLAGATLTVALITVWVRLLGAVGLLTTWLLFG
                     SLTVVAAAVIVTVRLRGVRWRLPWRRAVSAETVAVLVVSAVAVVIAVVAGYYLPVWQW
                     DALGYHLPYVNFALQRGTLADLPSDMPYLSTYPHIVEYLFIAWRAMLPDDRLVDLTGV
                     PLGLLGALAVATIARGQGARIAHAVAAGAAWLTLPAVFLQLPTNYVDVAAAAFLLTAA
                     AFLLGPLDVRRVLLAGVAIGLFLGAKPTALLGAALLLAALAFRARREGVLRWVAPAVV
                     LTTVLGAETYVVNLIRHANPIWPVRVDVGAVHLPGTHTMSELLASGANTPRTHGNPLQ
                     RVVESWTTVFPPLPVFDMRVGGLGLVFLIALPFAVVRAVRARSLTVALVAAASLAVPD
                     PSIARFVLGFAGLVIALAVPCVEHVRKPAHWAVFGVVALAAVQSIAVAYPGLRGEGPP
                     LTAYPRMDLAERQRAVGADGSPAPFLDAIAHVRPGEITVFDRSAELPYYAWPFDLSRD
                     AQRIPDDATAAEIRHIVDDPTVRMVIVGDDTVAGAVVHADPRFTYQFHCHTGTCAAYL
                     RR"
     gene            2111..>2328
                     /locus_tag="VF874_15845"
     CDS             2111..>2328
                     /locus_tag="VF874_15845"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9177476.1"
                     /translation="MTEDDDPRPGPGEVCVRMLASGVSLSDAQMRAGTYLGGPKPPFT
                     PGYELVGVVEALGPGCSSRLRVGDRVAAL"
BASE COUNT          315 a          760 c          809 g          444 t
ORIGIN      
        1 tggcgcgctg gtactgccgc gggccggaat gcagtcggtg ttggccggcg cgacgctgac
       61 ggtggcgctg atcacggtgt gggtgcggct gctcggcgcg gtgggtctgc tgacgacctg
      121 gctgctgttc ggctcgctga cggttgtcgc cgccgccgtc atcgttaccg tacggctacg
      181 cggtgtgcgg tggcgactgc catggcgtcg tgcggtcagc gcggaaaccg ttgccgtact
      241 cgtggtttcg gccgtcgcag tggtcatcgc ggtcgtcgcc ggatactacc tgccggtgtg
      301 gcaatgggac gcgctcggct accacctgcc gtacgtgaac ttcgcgctgc agcgcggcac
      361 gctggccgat ctgccgtcgg acatgccgta tctgtccacc tatccacaca tcgtggaata
      421 tctgttcatc gcgtggcgcg caatgcttcc cgacgatcga ttggtcgacc tgaccggtgt
      481 gccactcggc ttgctcggcg cgctggcggt cgccaccatc gcccgcggtc agggcgctcg
      541 gatcgctcac gctgtcgcgg caggcgctgc gtggctgacg ctgccagcgg tgttcctgca
      601 gctgccgacg aactacgtgg acgtcgccgc cgccgcgttc ctgctgacgg ccgcggcgtt
      661 cctgctgggg ccgttggatg tgaggcgagt attgctggcc ggcgttgcta tcgggctgtt
      721 tctgggcgcc aaacccaccg cgctgctcgg tgctgcgctg ctgttggcgg cgttggcttt
      781 tcgtgcccgt cgcgaaggtg tgctgcggtg ggtcgcgccg gcagtggtcc tcacaacggt
      841 cctgggcgcc gaaacgtatg tggtgaacct gattcggcat gccaacccga tttggccggt
      901 tcgggtggat gtcggggccg tgcatctacc tggcacacac acgatgtcgg aactgcttgc
      961 ctccggcgcg aacaccccgc gcacgcacgg caacccgctg cagcgggtcg tcgagtcctg
     1021 gacgacggtc ttcccgccgt tgccggtgtt cgacatgcgc gtcggcggat tggggctggt
     1081 gttcctcatc gctctgccat tcgcggtcgt ccgcgccgtg cgcgcgcgtt cgctgaccgt
     1141 ggcgctcgtc gcggcggcgt cgctggctgt gcccgacccg tcgatcgcga ggttcgtgct
     1201 cggcttcgcc ggccttgtga tcgcgttggc ggtgccctgc gttgagcacg tccgaaagcc
     1261 ggcgcactgg gcggtgttcg gcgtcgtcgc gctcgccgcg gtgcagagca tcgccgtggc
     1321 gtacccaggc ttgcgcggcg aaggaccgcc gctcaccgcc tacccgcgca tggacctagc
     1381 cgaacgacag cgcgcagtcg gcgcggacgg gtctcccgcg ccgttcctcg acgcaatcgc
     1441 ccacgtgcgc cccggtgaga tcaccgtctt cgaccgctcg gctgaacttc cctactacgc
     1501 ttggcctttc gacctgtcgc gcgatgcgca gcgaatcccc gacgacgcga cggctgccga
     1561 gatacggcac atcgtggacg acccgaccgt gcggatggta atcgtcggcg acgacaccgt
     1621 ggccggggca gtggtgcacg cagatccacg gtttacgtac caatttcatt gtcacacagg
     1681 cacatgcgcg gcatacctgc gccgctagcc gcacggtgtc cggcattgtc gcgttggccg
     1741 atcggctacc ggcgtcgtga tgtgtcgccg atttccggcg tgacctcgcg gcgggggcgg
     1801 agctgcgacg gggtaagaag tgacggggta agaagtgacg aacggcagtc gaagtgagga
     1861 cttcagaccc tggcgtggac gccgcgcaca gccaaacagt ggaggcgcta attgcatctt
     1921 gcttgagcac gcaggaggca acaaatgcgg accgacgatc cccgtcggcg taatcgcggc
     1981 ctcgccgacc cgctgctccc tttcaactac gcggaacaat tatcgcgcgc atcggcaacc
     2041 ctggcaaaga ggaggtcgtc tcatgaagcg tgtcattgtc gaccatttcg gaggccccga
     2101 agttgtcagc gtgacggagg acgacgatcc ccgacccggt ccgggcgagg tctgtgtccg
     2161 gatgctggcc tccggcgtat cacttagcga tgcccagatg cgcgccggca cgtatctggg
     2221 tgggccgaag ccgccgttca cccccggcta cgagctcgtc ggcgttgtcg aggcactcgg
     2281 ccccgggtgc tcatccaggc tgcgagtggg agaccgggtg gcggcgct
//
LOCUS       DASWBF010000428         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_631538,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000428 DASWBF010000000
VERSION     DASWBF010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631538"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VF874_15850"
     CDS             complement(<1..742)
                     /locus_tag="VF874_15850"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9177477.1"
                     /translation="MEHGTTLLFGLAGVAVREVSLRVDGTRVVDVVTDDVTAAACPTC
                     GVFSSARKGRVVARPMDLPYGEVGLRVRWHKSRWRCREPLCPRRSFTETIAEVPAGAR
                     TTGRLRRAVADGVADNRCVSEVAASYGLSWPTAQRAVAARAAEQAGPPAPTPVLGIDE
                     TRRERARWVRTADGGWRRTDPWESEFVDLATGQGLVGQVGGRSGASVEMWLAEQTEAF
                     RDAIRYVALDPSAPYASAVRRDLPQATVV"
     gene            1101..1913
                     /locus_tag="VF874_15855"
     CDS             1101..1913
                     /locus_tag="VF874_15855"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX9177478.1"
                     /translation="MGTSRGTKRYPTDLTDAQWGEIEGFLDPPRDLSRGGRPQQYPRR
                     RIADTVFYQLRTGVQWHLLPKDLPPWTAVWRQFCRWRDTGIWTLIMQHLLRVCRVEAG
                     RKPEPSAALLDAQSVPSGCLGPREHVGVDGGKRVRGRKRHLLCDLTGLPIAINVTSAQ
                     PHDSRGGWTLLKTVKPQVNRPTKVFADSAYHGLAARAEQELGLSIDIRKRPDGTHWFV
                     PLQPLWRVERTFAWLGRNRRLLYDYEATVASSEAFVHAGAVALMLGRLHPHR"
     gene            2106..2228
                     /locus_tag="VF874_15860"
     CDS             2106..2228
                     /locus_tag="VF874_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177479.1"
                     /translation="MAQPGPRPLVTPSSERAEAQQRRFADLAGVGHLVVLSQLA"
BASE COUNT          384 a          894 c          717 g          345 t
ORIGIN      
        1 ccaccacggt ggcctgcggc agatcccggc gcaccgccga ggcgtatggc gccgacgggt
       61 ccaacgcgac atagcggatc gcgtcccgga acgcctcggt ctgctcggcc agccacatct
      121 ccacactggc ccccgaccgc ccgccgacct ggcccaccag gccctgcccg gtcgccaggt
      181 cgacgaactc gctctcccac gggtcggtgc gccgccaccc gccgtcggcg gtgcgcaccc
      241 accgggcccg ttcccgccgc gtctcgtcga tccccagcac cggcgtcggc gccggcggcc
      301 cggcctgctc ggcggcccgg gccgcgaccg cccgctgcgc ggtcggccag gacagcccgt
      361 agctggccgc cacctcgctg acacaccggt tgtcggccac gccgtcggcg accgcccgcc
      421 gcagccggcc ggtcgtgcgc gcccccgccg gcacctcggc gatcgtctcg gtgaacgacc
      481 gccgcgggca caacggctct cggcaccgcc accggctctt gtgccagcgc acccgcagcc
      541 caacctcccc gtagggaaga tccatcggcc gtgcgaccac cctgcccttg cgggccgagg
      601 agaacacccc acacgtcggg cacgcggccg ccgtcacgtc atcggtgacg acatcgacca
      661 cccgagtgcc gtcgacacgc agactcacct cccgcacggc aaccccggcc aacccgaaca
      721 gcagcgtcgt accgtgttcc aagcccgtga cctcccacct cgctgaaccg aagactcagc
      781 aagggtcaag cggtcacggg ccccctcatc ggaggcgcgc ggtagccggt tgcccggtca
      841 aagttggaga gccccattga cagttcgtcc attggaaagt ccagccggct cggcacgcgc
      901 ctcatccgtc aacacgccac attgaacaac cgtcacactc acattgttgt agaaacccgg
      961 cccgtagtca gggcgacatg cttacgcgac actaggcact gtagagccca tggacaaacc
     1021 tttgacctgg ggaaacgttc tatgagggca ggtcaggctg cccgttcgga tggctggttg
     1081 gtgccgcacg ttggtgtcgt atgggcactt cacgtgggac gaagcggtac ccgacggact
     1141 tgacggacgc gcagtgggga gagatcgagg ggttccttga tccgccacgt gatctgtcgc
     1201 ggggtgggcg accgcagcag tatccgcgcc gccggatcgc cgacaccgtg ttctaccagc
     1261 tcaggacagg tgtgcagtgg catctgctac ccaaagatct cccgccgtgg acggccgtgt
     1321 ggaggcagtt ctgccggtgg cgcgacaccg gcatctggac cctgatcatg caacatctgc
     1381 tgcgggtctg ccgggtcgaa gccggccgga aaccggagcc gtccgccgcg ctgctcgacg
     1441 cccagtcggt gccctccggt tgcctcggcc cccgcgaaca cgtcggtgtc gacggcggga
     1501 aacgtgtccg cggccgcaaa cggcatctgc tctgcgacct caccggtctc ccgatcgcga
     1561 tcaacgtgac cagcgcccaa ccgcacgact cacgtggcgg atggaccctt ctcaagacgg
     1621 tgaaaccgca ggtcaaccgc cccaccaagg tgttcgccga cagcgcctac cacggcctgg
     1681 ccgcacgcgc agagcaagaa ctcggcctct ccatcgacat caggaaacgt cccgacggca
     1741 cccactggtt cgtccccctc cagccgctct ggcgagtcga acgaaccttc gcctggctcg
     1801 gccgtaaccg ccggctcctc tacgactacg aagcaaccgt cgcctcctcc gaagccttcg
     1861 tccacgcggg cgctgtcgcc ctcatgctgg gccgactcca cccccaccga tagccgtttc
     1921 cccaggtcag aggtttgtcc atgggctctg acaccaggtc accgcagtcg actacgcacc
     1981 gaaaggctgg ccacccacag tcgcgcacgc gatccggtgc ccggcatggc gcagatagcg
     2041 gacctgcaag gcgtccaacc cggccaccgg cctggaatcc acctcgatcg gcgacggtgg
     2101 aggctgtggc acagcccgga cctcgaccgc tggtcacgcc ctcctccgag cgggccgagg
     2161 cgcagcaacg acgcttcgcc gacctggccg gtgtcggtca cctggtggtg ctctcccagt
     2221 tggcatgagc actcaccggt gcggttcctg cgctatcacg ccgcggtcga acggcatgtc
     2281 cgtgacctgc atcgcctacg cttgccggcg tgcggttgca ggttgagcgg gccgaactcg
//
LOCUS       DASWBF010000429        15989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_895020,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000429 DASWBF010000000
VERSION     DASWBF010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15989
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895020"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..485)
                     /locus_tag="VF874_15865"
     CDS             complement(48..485)
                     /locus_tag="VF874_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9177480.1"
                     /translation="MTDPETEQRTAGIVSRGVAAVVDFLVVGALLAALYLGLVLTRLM
                     FHSTAFSFPSLNVVFSSAVAFVVSVLYLVGCWSVSGCTAGAVLMGLRVVGRRSDRLRL
                     LVALLRAITCVLFPFGLLWVAVDRQRRSLHDIVFGSRVIYARP"
     gene            complement(482..1003)
                     /locus_tag="VF874_15870"
     CDS             complement(482..1003)
                     /locus_tag="VF874_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177481.1"
                     /translation="MPELAELRKQPVVLVAAGAAATVAGGAVAAIRVCEQVPVVGGWL
                     RRGRLELAQRGEEAITRYAEPLKALVSAVAVDIVRLVLDQLDLTEVVREAVDIDAIVS
                     DIDIDAIVGRLDLIGLANDVIEGVDLPAIIRESTDTVTAEVITDVRAQGERADDMVSG
                     FVDRMLGRGRETR"
     gene            complement(1119..2669)
                     /locus_tag="VF874_15875"
     CDS             complement(1119..2669)
                     /locus_tag="VF874_15875"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9177482.1"
                     /translation="MSAGTTAEKLAELRAKMELAKEPGGEKAAAKRDKKGIPSARARI
                     HALVDPGSFLEIGALARTPGDPNALYGDGVVTGHGTINGRPVGLFSHDQTVFGGTVGE
                     MFGRKVSRLMEWVAMVGCPIVGINDSGGARIQDAVTSLAWYAELGRRHELLSGLIPQI
                     SIILGKCAGGAVYSPIQTDLIVAVRDQGYMFVTGPDVIKDVTGEDVSLDELGGADYQA
                     RYGNIHQVVEDEAAAFKYVRDFLEFLPANSFDDPPVINPGMEPEITPHDLELDLIVPD
                     SDNMAYDMHEILLRIFDDGDFLDVAAQAGQAIITGYARVDGRPVGVVANQPMHMSGAI
                     DNEASDKAARFVRFCDSFNIPLVFVVDTPGFLPGVEQEKNGIIKRGGRFLYSVVEADV
                     PKVTITIRKSYGGAYAVMGSKQLTADLNLAWPTARIAVIGAEGAAQLLMKRFPDPNAP
                     EAQEIKRNFIEGYNLNMATPWIAAERGFIDAVIEPHETRLHLRKAMHLLRGKQIQRVQ
                     RKHGLIPI"
     gene            complement(2666..7945)
                     /gene="pks13"
                     /locus_tag="VF874_15880"
     CDS             complement(2666..7945)
                     /gene="pks13"
                     /locus_tag="VF874_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242497.1"
                     /note="Pks13 is a key enzyme in mycolic acid biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase Pks13"
                     /protein_id="HEX9177483.1"
                     /translation="MAENDFEDSSPQPDVVADAGVPPPGDLTVVEMRAWLRNWVASAT
                     GQSADAIDETTPMVELGLSSRDAVAMAADIEDLTGVTLSATVAFQHPTIESLATRIVE
                     GEPEPEGVDEDEDWSRTRDVPDIAIVGLSTRFPGDMNTPEETWQALLQGRSGISDLPE
                     GRWSEFLEEPRIAERVAKARTRGGYLKDIKGFDSEFFALSKMEADNLDPQQRMALELT
                     WEALEHARIPASSLRGESVGVYIGASNNDYSFLAVSDPTVAHPYAITGTASSIIANRV
                     SYFYDFRGPSVAVDTACSSSLVAAHQAVQALRVGECDVALAGGVNALITPMITIGFDE
                     VGGVLAPDGRIKSFSSDADGYARSEGGGMLVLKRVDDARRDGDQILAVIAGSAVNHDG
                     RSNGLLAPNPDAQADVLRKAYKDAGINPRTVDYIEAHGTGTILGDPIEAEALGRVVGR
                     GRPADRPALLGAVKSNVGHLESAAGAASLAKMALALSHDKLPPSINYAGPNPYIDFDA
                     VHLKVADTVTDWPRYSGYAITGVSGFGFGGANAHLVLREVLPRDVIEKVPEPEPAAEE
                     TVVEEAAAEYVGGVRFDEYGEFIDDDLPEAAAEPEYELPGLTDEALRLKEIALEELEA
                     AEQPVPVVPLAISAFLTSRKKAAAAELADWIDSPDGRASSLESIGRALSRRNHGRSRA
                     VVLAHDHDEAIKGLRAVAEGKQRPNVFTADGPVTNGPVWVLAGFGAQHRKMAKNLYLR
                     NDVFAEWIDKVDALVQDERGYSIVELILDDAQDYGIETTQTVIFGIQVALGELLRHHG
                     AKPAAVIGQSLGEAAAAYFAGGLSLADATRTICSRAHLMGEGEAMLFGEYIRLMALVE
                     YSADEIKTVFSDFPDLEVCVYAAPTQTVIGGPPEQVDAIIARAESEGKFARKFQTKGA
                     SHTQQMDPLLGELAAELIGIEARPTTIGYYATVHEGSFIRPGQQIHDVDYWKKGLRHS
                     VYFTHGIRNAVDNGHTTFLELAPNPVALMQVGLTTAAAGLHDAQLIATLARKQDEVES
                     MVAAMAQLFVYGHDLDIRTLFTRADGPADYANIPPTRFRRKEHWLDAHFSGDASVVMP
                     GNHVATPDGRHIWEYKPRGETDLAALVKAAAAQVLPDAQLTASEQRAVPGDGARLVTT
                     LTRHPGGAAVQVHARIDESFTLVYDAIVSRSGLVSALPAAVAVGTVAPQPVTEAPASE
                     PEEQPDAEILHDNLIQGANLGADFAKWSPDSGESVGQRLATIVSNAMGYEPEDLPWEV
                     PLIELGLDSLMAVRIKNRAEYDFDLPPIQLQAVRDANLYAVEKLIQYAIEHRDEVQQL
                     HDYQKTKTADEIAEEQARLLSGASVETATAAAPDQAEPETQNIPPPPPSDNPAGPRRE
                     KANLAAEAQALNQKAVADALGSDVPPRDASERVTFATWAIVTGKSAGDIFNALPRIDE
                     ATAEKMAQRLSERADGPITAEDVTTSPTIEALAEKVRNYLEAGQIDGFVRIIRAREEG
                     SDKVPVFVFHAAGGSTVVYEPLLKRLPADTPMYGFERVEGTIEERAAQYLPKLRELQG
                     DGPFILVGWSLGGVLAYACAIGLKEQGCDVPFVGLIDVVRAGEEIPQTKEETRKRWER
                     YARFAERTFNVQVPDIPYEHLETLDDAGQVEFVLNAVKDSGVQIPGGIIEHQRTSYLD
                     NRAIDTAEIRPYDGHVTLYIADRYHDDAIMFEPRYATRKPDGGWGEFVSDLEVVPIGG
                     EHIQAIDEPIIAKVGAHMSQAITKVENETKAAK"
     gene            complement(7965..9869)
                     /gene="fadD32"
                     /locus_tag="VF874_15885"
     CDS             complement(7965..9869)
                     /gene="fadD32"
                     /locus_tag="VF874_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--AMP ligase FadD32"
                     /protein_id="HEX9177484.1"
                     /translation="MPIWVNPFIDKTTGRIKFPEGASIVRHVERWAQTRGDHVVYRFL
                     DFSTERDGVPRDLTWSQFSARNRALAARLQQVTQPDDRIAILCPQNLDYLTAFYGAIY
                     SGRLAVPLFDPAEPGHVGRLHAVLDDCTPSVILTTTGSAEGVRKFFHKRPAKERPRII
                     AVDAVPDEVADTWVRPDNPTEDTIAYLQYTSGSTRSPAGVQITHQSVATNLVQLMDGL
                     QAEEGQRGVTWLPFFHDMGLLTIMLPSIIGRQITFMTPAAFVRRPIRWMRELGVQDSD
                     HGRTFSAAPNFAFEHAALRGLPKEGEPPLDLSNELAILNGSEPISQVSMRKFVDAFAP
                     YGLTEAAIKPSYGMAEATLFVSTTDMKVGPTVIRVNRDELNKGKLVKVSADSPDGVPQ
                     VSCGRISYDQWATIVDADTATELPDGHIGEIWLHGNNMGSGYWNREEETRETFQNILK
                     SRTNPSHAEGAPDDAMWMRTGDLGAFLDGEFYITGRIKELVIIDGRNHYPQDLEHTAH
                     EATRALRPGFAAAFSVPANQLSQAVFDNPHAGLKYDPENTSEQLVIVGERAPGTHKLD
                     VEPIADDIRAAVAVRHGVTVRDVLLMPAGTIPRTSSGKIGRRACRAAYIDGSLRGGTA
                     SPNSFPDQAD"
     gene            complement(10116..11024)
                     /locus_tag="VF874_15890"
     CDS             complement(10116..11024)
                     /locus_tag="VF874_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEX9177485.1"
                     /translation="MATRRRRNRRILIAVVGVLLLAGLVAVFVLRKPRPEVQGAGCPD
                     VQLVSVPGTWESSPQLDPVNPVQFPNALLLNVTRPLTEQLDGDRLQAYTVPYIAQFHR
                     PLASDNEVDYDVSRRQGTDVTRRAIADIASRCPLTSYVIVGFSQGAVIAGDIASDIGN
                     GRGPVDPDRVLGVTLIADGRRQQGVGQDIGPNPPGQGAEVTLQNVPFIPSGITMTGPR
                     EGGFGALNDRTNQICAQGDLICASPDEAFNFLSLPNTLQVLLGAAGEPVHAMYNTTQF
                     WNFGGQPATEWTRDWAADVIKNAPQP"
     gene            complement(11027..12043)
                     /locus_tag="VF874_15895"
     CDS             complement(11027..12043)
                     /locus_tag="VF874_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEX9177486.1"
                     /translation="MANTNRRKRHRILALVAAGAVAVLVVLGLVYLVYYLRAPSGEGP
                     PEATAPNAQPPTTSVVPKKPRPEFQDASCADVQVLSIPGTWESAPQQDPLNPVQFPNA
                     LLLNVTGPLTEQFGSDRIQAYTVPYTAQFHNPFARDGQISYNDSRQEGMDAAVRALTD
                     MTNRCPLTSYVIVGFSQGAVIAGDIASDIGNGRGPVDEDLVLGVTLIADGRRQSGVGQ
                     DVPPTPVGQGAEITLREVPMLSGMGLTMTGPRLGGFGALNNRVFEICAQDDLICAAPQ
                     EAFNIVNLPSTLELLAGGAGQPVHALYNTTQFWSTPDGQPATVWTRNWAADAVEKAPH
                     PKHG"
     gene            complement(12053..12547)
                     /locus_tag="VF874_15900"
     CDS             complement(12053..12547)
                     /locus_tag="VF874_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9177487.1"
                     /translation="MQRTSTRMRGPSTVATLALATAVLSGCSANDGLLSSVTLPADDT
                     TTALVSGRQDPGASPAPPPAGSSGSETVNKLTVTGQQRAYLDALAAGGVHPSSELLAL
                     SIGSYVCQARAAGQTPQAVWDFVHPLVSSDVHDAHMSSMPPTPADVDAATGSYIRVAT
                     ERLC"
     gene            complement(12624..13520)
                     /locus_tag="VF874_15905"
     CDS             complement(12624..13520)
                     /locus_tag="VF874_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX9177488.1"
                     /translation="MKVGVVLVRALLVAILGLALWTAPAMSGTAKADSVEMLMVPSGA
                     MGRDIPVAFYNGGPHAVFLLDAFNAGDGVSNWVTAGNAMNTLAGQGISVVAPAGGAYS
                     LYTNWEQDGSRQWETFLSQELPDWLAANRGLAPNGHGIVGAAQGGTGAITLAAFYPDR
                     FRFAGSLSGFLTPSATAMNGAITDGLMRFGGVDSRNMWGLPQLGRWKWHDPDVHISLL
                     AQNNTRLWVFSPQTLTCSDPAAMISYCDQAQGSNRSFYQHYRQVSGHNGHFDFPPSGN
                     HDWSTWGPQLGAMAGELVATIK"
     gene            complement(13666..14652)
                     /locus_tag="VF874_15910"
     CDS             complement(13666..14652)
                     /locus_tag="VF874_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX9177489.1"
                     /translation="MKFVEKLRGRWARRLTIAAMAAVLLPGLVGVVGDSATAGAFSRP
                     GLPVEYLDVPSASMGRDIRVQFQSGGANSPAVYLLDGLRARDDYSGWDIETNAFEMYY
                     QSGLSVVMPVGGQSSFYSNWYKQACGKSGCLTYNWETFLTSELPAYLAANKQVKPTGS
                     AAVGLSMAGSAAMTLAIYYPQQFPYAASLSGFLNLSEGWWPMLVGMSMGDAGGYKSED
                     MWGPSSDPAWKRNDPMVNVSKLVANNTRIWVYCGNGKPNELGGGDFNAKFLEGFTLRT
                     NKSFKDAYIAAGGTNGVFNFPEAGTHSWVYWGAQLNAMKPDIQRVLGATPTA"
     gene            complement(15008..>15989)
                     /gene="zomB"
                     /locus_tag="VF874_15915"
     CDS             complement(15008..>15989)
                     /gene="zomB"
                     /locus_tag="VF874_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor control protein ZomB"
                     /protein_id="HEX9177490.1"
                     /translation="LVQSPTAVVVFIVVSGLLQALYWIRQGGDFMHGRVLLTPLFCVL
                     APVAVIPVVLPERMKLSRGRGVVLAGATAAVWLAVAVWALWAANSPGMGTDATRVTYT
                     GIVDERRFYAQATGNAHPLTAADYLDYPRMRAAIEAIENTPDGALLLPSGNYTQWDVV
                     PADPAELGQRADKNGPHTVFFTNLGMLGMNVGLDVRVVDQIGLANPLAAHTARLQDAR
                     IGHDKNLFPDWAVAEGPWLKEHPYLPPYLDEDWVTQAEEALKCPATDAVLTSIRGPLT
                     MRRFLSNLLHAHQFTSYRIDRVPRYELIRCGLSEPPSRGTPYNGLPATGP"
BASE COUNT         2476 a         5445 c         5327 g         2741 t
ORIGIN      
        1 tcgccggtac gggcgagcgg cgtggtcacc ggctcacgct accgcgatca gggtcgcgcg
       61 tagatcaccc tgctgccgaa cacaatgtcg tgcagcgacc ggcgctgacg gtcgaccgcg
      121 acccagagca acccgaacgg aaacagcacg caggtgatcg cccgtagcaa cgcgaccaga
      181 agtctgagcc ggtccgagcg gcggccgacc acccgcagtc ccatcaggac cgcgccggcg
      241 gtgcagcctg acaccgacca gcagcccacg aggtacagca ccgacacgac gaatgccacc
      301 gcgctggaga agacgacatt cagcgacggg aaactgaacg ccgtcgagtg aaacatcagc
      361 cgcgtcaaca ccagaccgag atagagcgcg gcaagcagcg ccccgacgac gaggaagtcg
      421 acgaccgcgg ccacgcctct gctgacgatg ccggctgtgc gctgctccgt ctcaggatcg
      481 gtcaccgggt ttcccggcca cgtcccaaca tccggtcgac gaagcccgac accatgtcgt
      541 cggcccgctc cccctgggcg cggacgtcgg tgatcacctc tgcggtcacc gtgtccgtcg
      601 actccctgat gatcgcgggc aggtctacgc cctcgatcac gtcgttggcc agcccgatga
      661 ggtcgagacg cccgacgatc gcatcgatgt cgatgtcgga cacgatcgca tcgatgtcga
      721 cggcttcacg caccacctcg gtcagatcca attggtccag gacgagtcgc acaatgtcga
      781 ccgcgacggc ggacacgaga gccttgagcg gctcggcata acgcgtgatg gcttcttcgc
      841 cgcgctgagc cagttcgagt cggccgcggc gcagccagcc gccgaccacc ggcacttgct
      901 cgcacacccg aatagctgcc acagcaccgc cggcaacggt cgcggccgct ccggcagcca
      961 ccaataccac aggctgcttg cgtagttccg caagttcggg caccaccgca tcgtagtctc
     1021 ggcgggcgtt ctgactcgcc gaaacagaaa tcatggtcgt gatccgcgct cgatcacagc
     1081 catggcttcg gtttcggcgc taggctagac gctcgaggct agatggggat caggccgtgc
     1141 ttgcgctgga cccgctgaat ttgcttgccg cgcaacagat gcatcgcctt gcgtagatgc
     1201 agccgggtct cgtgcggctc gatcaccgcg tcgatgaacc cgcgctcggc ggcaatccac
     1261 ggggtcgcca tgttgaggtt gtagccctcg atgaagttgc gcttgatctc ctgcgcctca
     1321 ggcgcattcg ggtcggggaa gcgtttcatc agcagctgcg cggcaccctc ggcgccgatc
     1381 accgcgatcc gggctgtcgg ccacgccagg ttcaggtccg cggtcagctg cttgctgccc
     1441 atcaccgcgt acgcgccgcc gtaggacttg cggatcgtga tcgtcacctt cgggacgtca
     1501 gcctccacca ccgagtacag gaaacgcccg ccgcgcttga tgattccgtt cttctcctgc
     1561 tcgacacctg gcaagaagcc gggggtgtcg acgacgaaca ccagcgggat gttgaacgag
     1621 tcgcagaacc gcacgaaccg tgccgccttg tcggacgcct cgttgtcgat cgcgccggac
     1681 atgtgcatcg gctggttggc caccacgccg accggccggc cgtcgacgcg ggcgtacccg
     1741 gtgatgatcg cctgcccggc ctgggccgcg acgtcgagga agtcgccgtc gtcgaagatc
     1801 cgcagcagaa tctcgtgcat gtcgtaagcc atgttatcgg agtccggcac gatcaagtcc
     1861 agctccaggt cgtgcggcgt gatctcgggc tccatgccgg ggttgatgac cggcggatcg
     1921 tcaaaactgt tcgcgggcaa gaactccagg aaatcgcgca cgtacttgaa cgccgcggcc
     1981 tcgtcctcga cgacctggtg gatgttgccg tagcgggcct ggtagtccgc accgccgagc
     2041 tcgtcgaggc tgacgtcttc accggtgacg tccttgatca cgtcgggccc cgtgacgaac
     2101 atgtagccct ggtcgcggac cgccacgatc aggtcggtct ggatcggtga atagactgct
     2161 cccccagcac atttgccgag gatgatggag atctgcggaa tcaggccgga gagcagctcg
     2221 tggcggcggc ccagctcggc gtaccacgcc agcgacgtca ccgcgtcctg gatccgcgcc
     2281 ccgccggagt cgttgatgcc gacgatcggg cagccgacca tcgccaccca ctccatcagc
     2341 cgagaaacct tgcggccgaa catctctccg accgtcccgc cgaacaccgt ctggtcgtgg
     2401 ctgaacaagc cgaccggtcg cccgttgatc gtgccgtggc cggtgacgac gccgtcgccg
     2461 tagagcgcgt tcggatcccc gggcgtgcgt gccagcgcgc cgatctccag gaagctgccg
     2521 gggtcgacca gcgcgtgaat gcgggcgcgg gcgctgggga tgcccttctt gtcgcgtttg
     2581 gccgcggcct tctcaccgcc gggttccttg gccaactcca tcttcgcgcg cagttcggcg
     2641 agtttctcgg cggtcgtgcc cgcgctcatt tcgctgcctt cgtttcgttt tcgaccttcg
     2701 tgatcgcctg gctcatgtgc gcgccgacct tggcgatgat cggctcgtcg atcgcctgga
     2761 tgtgctcgcc gccgatcggc accacctcga gatccgacac gaattcgccc cagccgccgt
     2821 ccggcttgcg ggtggcgtat cgcggctcga acatgatcgc gtcgtcgtgg tagcggtcgg
     2881 ctatgtacag cgtgacatgc ccgtcgtagg gccggatctc ggcggtgtcg atcgcgcggt
     2941 tgtccagata cgacgtgcgc tggtgctcga tgatgccgcc ggggatctgc accccactgt
     3001 ccttgacggc gttcagcacg aactcgacct ggccggcgtc gtcgagggtt tccaggtgct
     3061 cgtacgggat gtccgggacc tgcacgttga acgtgcgctc ggcgaaccgg gcgtaacgtt
     3121 cccaccgctt gcgggtctct tccttggtct gcgggatctc ttcgccggca cgcaccacgt
     3181 cgatcagccc gacgaacggc acgtcgcagc cctgctcctt gagcccaatc gcacacgcgt
     3241 acgcgagtac gccgcccagc gaccagccga ccagaatgaa cgggccgtca ccctgcagct
     3301 cgcgcagctt cggaaggtat tgcgcggcac gctcttcgat ggtgccctcg acacgctcga
     3361 acccgtacat cggggtatcc gccggcagcc gcttcagcag cggctcgtag acgacggtcg
     3421 agccgcccgc ggcgtggaac acgaataccg gcaccttgtc ggagccctct tcccgcgccc
     3481 tgatgatgcg gacgaacccg tcgatctgcc ccgcctcgag gtagttacgg accttttcgg
     3541 ccagcgcctc gatggtcggc gacgtcgtca cgtcctcggc ggtgatcggg ccgtcggcac
     3601 gctcggagag ccgctgcgcc atcttctccg cggtcgcctc gtcgatgcgg ggcagcgcgt
     3661 tgaagatgtc accggctgac ttgccggtga cgatcgccca cgtcgcgaac gtcacccgct
     3721 cggacgcgtc gcgcggcggc acgtcggagc ccagcgcgtc ggcgaccgcc ttctggttca
     3781 gggcctgcgc ctcggcggcc aggttcgcct tctcccttcg cgggcctgcg ggattgtccg
     3841 acggcggcgg tggcgggatg ttctgtgtct ccggttcggc ctggtccggc gccgctgccg
     3901 tcgcagtctc caccgacgcg ccggacagca gacgcgcctg ctcttcggcg atctcgtcgg
     3961 cggtcttcgt cttctggtag tcgtgcagct gctgcacctc gtcgcggtgc tcgatcgcgt
     4021 actggatcag cttctcgacc gcgtacaggt tcgcgtcgcg cacggcctgc agctggatcg
     4081 gcggcaggtc gaagtcgtac tctgcgcggt tcttgatccg caccgccatc agcgagtcca
     4141 gacccagctc gatcagcggc acctcccacg ggagatcctc gggctcgtag cccatcgcgt
     4201 tggacacgat cgtcgccaag cgttgcccga cggactcgcc ggagtctggc gaccacttgg
     4261 cgaaatcggc gcccaggttc gcaccctgga tgaggttgtc gtgcaggatt tcggcatcgg
     4321 gctgttcttc gggttccgat gcaggggcct cggtaacagg ttgcggcgca acggttccga
     4381 cggccaccgc cgcgggtagc gccgagacca gacccgaccg ggacacgatc gcgtcgtaga
     4441 cgagcgtgaa tgactcgtcg atgcgggcgt gtacctgcac cgccgccccg ccgggatgcc
     4501 gggtcagcgt cgtcaccagc cgggctccgt cccccggcac ggcgcgctgc tcgctcgcgg
     4561 tcaactgagc atccggaagc acctgcgccg cagcggcttt caccaacgcg gccaggtcgg
     4621 tctcgccgcg cggcttgtac tcccagatgt ggcggccgtc gggcgtcgca acgtggttgc
     4681 ccggcatgac gaccgatgcg tcaccggaga aatgcgcgtc gagccagtgc tccttgcggc
     4741 ggaatcgcgt cggcgggatg ttcgcatagt cagcgggccc gtcggcacgc gtgaacagcg
     4801 tgcggatgtc gaggtcgtgg ccgtacacga acagctgcgc catcgcggcg accatcgact
     4861 cgacctcgtc ttgcttgcgc gcgagcgtcg cgatcagctg cgcgtcatgc aggccggccg
     4921 ccgcggtggt caggcccacc tgcatcagcg cgaccgggtt gggcgccagc tccaggaacg
     4981 tggtgtgccc gttgtcgacg gcgttgcgga taccgtgcgt gaagtacacg ctgtgccgca
     5041 gacccttctt ccagtagtcg acgtcgtgga tctgctggcc ggggcggatg aagctgccct
     5101 cgtgcacggt cgcgtagtag ccgatcgtgg tgggccgggc ctcgatgccg atcagctcgg
     5161 ccgccagctc gcccaacagc gggtccatct gctgcgtgtg gctggcgccc ttggtctgga
     5221 acttgcgggc gaacttcccc tcggactcgg cgcgggcgat gatcgcgtcc acctgctcgg
     5281 gcggcccgcc gatgacggtc tgggtcggcg cggcgtagac gcacacctcc agatccggga
     5341 agtcggagaa cacggtcttg atctcgtcgg cggagtactc gaccagtgcc atcagccgga
     5401 tgtactcgcc gaacagcatc gcctcgccct cacccatcag gtgggcgcgg gaacagatcg
     5461 tgcgggtggc gtcggccagc gacagcccgc cggcgaaata ggccgcggcc gcctcgccca
     5521 gcgactggcc gatcaccgcg gccggcttcg cgccgtggtg gcggagcagc tcgccgagcg
     5581 cgacctggat cccgaagatc acggtctgcg tggtctcgat gccgtagtcc tgcgcgtcgt
     5641 cgaggatcag ctcgacgatc gaatagccgc gctcgtcctg caccagcgcg tcgaccttgt
     5701 cgatccactc ggcgaacacg tcgttgcgca ggtacaggtt cttggccatc ttgcggtgct
     5761 gcgcaccgaa gccggccagc acccacaccg gcccgttggt caccggcccg tcggcggtga
     5821 acacgttcgg ccgctgcttg ccctcggcga ccgcgcgcag gcccttgatc gcctcgtcgt
     5881 ggtcgtgggc cagcaccacc gcgcgggagc ggccgtggtt gcgccgcgac agcgcacggc
     5941 cgatcgactc cagcgacgac gcgcgaccat ccgggctgtc gatccagtcg gccagctcgg
     6001 ccgcggccgc cttcttgcgc gacgtcagga acgccgaaat cgccagcggg acaacgggaa
     6061 ccggttgttc agccgcctcg agttcttcga gcgcgatctc cttgagccgc aacgcctcgt
     6121 cggtcagccc cggcaattcg tactcgggct cggcagcagc ctcgggcagg tcgtcatcga
     6181 taaattcgcc atattcgtcg aaccgcaccc cgcccacgta ctccgccgcc gcttcctcga
     6241 cgaccgtctc ctcggcggcc ggttcaggct ccggtacctt ctcgatcacg tcgcggggca
     6301 gcacctcgcg cagcaccagg tgcgcgttgg cgccgccgaa gccgaagccg gacacgccgg
     6361 tgatcgcata tccgctgtag cgcggccagt cggtgacggt gtcggcgact ttcaggtgca
     6421 ccgcgtcgaa gtcgatgtac gggttgggcc cggcgtagtt gatcgacggc ggcagcttgt
     6481 cgtgcgacag ggccagcgcc atcttggcga ggctggccgc gcccgcggcc gactccaggt
     6541 gcccgacgtt ggacttcacc gcacccagca gcgccggcct gtcggcggga cggccccggc
     6601 cgacgacgcg gcccagcgcc tcggcctcga tcgggtcacc gaggatggtg ccggtgccgt
     6661 gcgcctcgat gtagtcgacg gtgcgcgggt tgatgccggc gtccttgtag gccttgcgca
     6721 gcacgtcggc ctgggcgtcg gggttcggcg cgagcaggcc gttggagcgg ccgtcgtggt
     6781 tgaccgcact gccggcgatt accgccagga tctggtcgcc gtcgcggcgg gcgtcgtcca
     6841 cccgttttaa cacgagcatg ccgccgccct cggagcgggc gtagccgtcg gcgtcggagc
     6901 tgaacgactt gatccggccg tccggggcca gcacgccgcc gacctcgtcg aaaccgatcg
     6961 tgatcatcgg ggtgatcagc gcgttgacgc cgcccgccag agcgacgtcg cactcgccga
     7021 cccgcagcgc ctgcacggcc tgatgcgcgg cgaccaacga gctcgagcac gcggtgtcga
     7081 ccgccaccga cggaccgcgg aagtcgtaga agtaggacac ccggttggcg atgatgctgc
     7141 tcgcggtgcc ggtgatcgcg tacgggtggg cgaccgtcgg gtcggacacc gccaggaagc
     7201 tgtagtcgtt gttggacgcg ccgatgtaga cgccgacgct ctcaccgcgc aggctcgacg
     7261 ccggaatcct ggcgtgctcc aacgcttccc aggtcagctc cagcgccatc cgctgctgcg
     7321 ggtcgaggtt gtcggcctcc atcttcgaca gcgcgaagaa ctccgagtcg aaacctttga
     7381 tgtccttcag gtagccgccg cgggtgcggg ccttggcgac gcgctccgcg atgcgaggct
     7441 cttcgaggaa ctccgaccag cgtccctcgg gcagatcgct gattccgctg cggccctgaa
     7501 gcagtgcctg ccaggtttcc tcgggcgtgt tcatgtcgcc ggggaagcgg gtggacagcc
     7561 cgacgatcgc gatgtcgggc acgtcgcggg tgcgcgacca gtcctcgtcc tcgtcgacgc
     7621 cctcgggttc cggctcgccc tcgacgatcc gggtggccag cgactcgatc gtcgggtgct
     7681 ggaacgcgac cgtcgccgac agcgtgaccc cggtcagatc ctcgatgtcg gcggccatcg
     7741 cgaccgcgtc gcgcgacgac agccccaact cgaccatcgg ggtggtctcg tcgatcgcgt
     7801 cggcggactg gccggtggcg ctggcgaccc agttgcgcag ccaggcgcgc atctcgacga
     7861 cggtcaggtc gcccggtggg ggcactcccg cgtccgcgac gacatcgggc tgcggtgaac
     7921 tatcctcaaa atcgttctct gccatacgtt ttcgccgtta ccagtcagtc cgcctggtcg
     7981 gggaaggaat tcggggaggc ggtaccgcct cgcaggctgc cgtcgatgta ggcggcccgg
     8041 caggcccggc gcccgatctt gccgctggac gtgcgcggga tcgtgcccgc gggcatcagc
     8101 agcacgtcgc gcaccgtgac accgtgccgc accgcgaccg cggcgcggat gtcgtcggcg
     8161 atcggctcga cgtcgagctt gtgcgtgccg ggcgcccgct cgccgacgat cacgagttgt
     8221 tcggaggtgt tctccgggtc gtacttcagc cccgcgtgcg ggttgtcgaa caccgcttgc
     8281 gacagctggt tggccggcac ggagaacgct gcggcgaagc cgggccgcag cgcgcgggtg
     8341 gcctcgtgcg cggtgtgctc gaggtcctgc gggtagtgat tgcggccgtc gatgatcacc
     8401 agctccttga tacggccggt gatgtagaac tcgccgtcga ggaacgcgcc caggtcgcca
     8461 gtgcgcatcc acatcgcgtc gtcgggcgca ccttcggcgt gcgacgggtt ggttcgcgac
     8521 ttgaggatgt tctgaaaagt ctcgcgcgtc tcttcttcgc ggttccaata gccagaaccc
     8581 atgttgttgc cgtgcaacca gatctcgccg atgtgcccgt cgggcaattc cgtcgccgta
     8641 tcggcgtcga cgatggtcgc ccactggtca tacgagattc gcccacagga cacctgcggc
     8701 accccgtcgg gggagtccgc ggagactttt acgagcttgc ccttgttcaa ctcgtcgcgg
     8761 ttgacgcgga tgaccgtcgg accgaccttc atgtccgtgg tggacacgaa caacgtcgcc
     8821 tccgccatcc cgtaggacgg cttgatcgcg gcctcggtca gaccgtatgg cgcgaatgcg
     8881 tcgacgaact tgcgcatcga gacctgcgag atcggttcac tgccgttgag gatggccaac
     8941 tcgttgctga ggtcaagcgg cggctcgcct tccttgggga gcccacgcaa cgcggcgtgc
     9001 tcgaacgcga agttcggcgc cgcggagaag gtgcgaccgt gatcgctgtc ctgcacgcca
     9061 agttcgcgca tccaccggat cggccgccgc acgaatgccg ccggggtcat gaacgtgatc
     9121 tgccggccga tgatcgaggg cagcatgatc gtgagcaggc ccatgtcgtg gaagaacggc
     9181 agccaggtga cgccccgctg cccctcttcg gcctgcaggc cgtccatcaa ttggaccagg
     9241 ttggtggcca ccgactggtg cgtgatctga acgcccgccg ggctacgggt cgagcccgag
     9301 gtgtattgca gataggcgat cgtgtcctct gtgggattgt cgggccgcac ccaggtatcg
     9361 gcgacctcgt cgggcactgc atcgaccgcg atgatgcgtg gacgctcctt ggccggtcgc
     9421 ttgtggaaga acttgcgcac accctcggcc gacccggtgg tggtgagaat caccgacggg
     9481 gtgcagtcat cgagcaccgc gtgcaaacgg cccacgtgac ccggctccgc ggggtcgaag
     9541 agcggcacgg cgagccggcc ggagtagatc gcgccgtaga acgcggtcag gtagtcgagg
     9601 ttctgcgggc acaggatcgc gatccggtcg tcgggttggg tgacctgctg aaggcgcgca
     9661 gcgagcgcgc ggttgcgggc ggagaactgc gaccacgtca ggtcgcgcgg aacgccgtcg
     9721 cgttcagtgg aaaagtcgag aaacctgtac acgacgtgat cgccgcgggt ctgtgcccac
     9781 cgctccacat gccggacgat gctggcgcct tccgggaatt tgatccgccc cgtcgtcttg
     9841 tcgatgaacg ggttgaccca aataggcacg tttttttctc ctgtcgaagc acaccggccg
     9901 gttgtcgcac caagcggccc ggccctcggg tcggctggca ttcgccgcga accctaaacc
     9961 tccaagatcg tacagaggtt caggccgctc tccgccgccg cgtggcgcgc cgcgcatagc
    10021 cgttgccgct cttagttatt tcttaatgtt aggtgacgtg ggatcgagcg gccaaatcga
    10081 cagtccgctc gtcggacaaa tgttgcccat ccgtgctacg gctgcggtgc gttcttgatc
    10141 acgtcagccg cccagtcgcg cgtccattcg gtggccggct gaccgccgaa gttccagaac
    10201 tgcgtggtgt tgtacatcgc gtgcaccggc tcgcccgccg cgcccagcag cacctgaagc
    10261 gtgttcggca ggctcaggaa gttgaacgcc tcgtcgggtg aggcgcagat cagatcgcct
    10321 tgcgcgcaga tctggttggt gcggtcattc agtgcgccga acccgccttc gcggggaccg
    10381 gtcatcgtga tgccggacgg gatgaacggc acgttctgca gcgtgacctc cgcgccctga
    10441 cctggggggt tcggcccgat gtcctgtccc acgccttgct ggcggcgacc gtcggcgatc
    10501 agcgtcaccc ccagcacccg gtccgggtcg acggggcccc tgccgttgcc gatgtcgctg
    10561 gcgatgtcac cggcgatcac cgcgccctgc gagaagccga cgatgacgta gctggtcaac
    10621 gggcacctgc ttgcgatgtc ggcgatcgcc ctcctggtga cgtcggtgcc ctgcctgcgg
    10681 ctgacgtcgt agtcgacctc gttgtcgctg gcgagcggcc ggtggaattg tgcgatgtag
    10741 ggcacggtgt aggcctgcag ccggtccccg tcgagctgtt cggtcagagg ccgagtgacg
    10801 ttgagcaaca acgcattcgg gaactgcacg gggttgaccg ggtcgagctg cggtgacgac
    10861 tcccaggtgc cggggaccga caccagttgc acgtcggggc agccggcgcc ctgcacctcc
    10921 gggcgcggct tcctgagcac gaaaacggcc acgaggcccg ccagcagcaa tacgccgacc
    10981 acggcgatca ggatgcgacg gttgcgtcgg cgcctcgtcg ccacgatcag ccgtgcttgg
    11041 gatgcggcgc tttctcgacc gcatccgccg cccagttacg cgtccacacg gtggccggct
    11101 ggccgtcagg ggtgctccag aactgcgtgg tgttgtacag cgcgtgcacc ggctggcccg
    11161 ccccgccggc cagtagctcc aacgtggaag gcaagttgac gatgttgaac gcctcctggg
    11221 gcgcggcgca gatcaggtcg tcctgggcgc aaatctcgaa cacgcggttg ttcaacgcgc
    11281 cgaagccgcc cagccggggg cccgtcatcg tcaggcccat cccggacagc atcggcacct
    11341 cgcgcagcgt gatctcggcg ccctgcccca ccggggtcgg ggggacgtcc tggcccaccc
    11401 cgctttggcg gcgcccgtct gcgatcagcg tcaccccgag caccagatcc tcatcgacgg
    11461 gtcccctgcc gttaccgatg tcgctggcga tgtcgccggc gatcaccgcg ccctgcgaaa
    11521 agccgacgat cacgtagctg gtcagcgggc acctgttggt catgtccgtg agagcccgta
    11581 ccgcggcgtc catgccttct tggcggctgt cgttgtagct gatctggccg tcacgcgcga
    11641 acgggttgtg gaactgggcg gtatagggaa ccgtgtacgc ctgaatccgg tcgctgccga
    11701 actgctcggt cagcggccca gtgacgttga gcagcaacgc attcgggaat tgcaccgggt
    11761 tcagcgggtc ctgctgaggc gccgactccc acgtgccggg aatcgagagc acctgcacgt
    11821 cggcgcagct ggcgtcctgg aactcgggac gcggcttctt tggaaccacg gaggtggtgg
    11881 gtggctgcgc gttgggcgcc gtggcttccg ggggcccttc cccgctcggc gcgcgcaggt
    11941 aatacaccag gtagaccaga ccaagcacaa ccaggaccgc caccgcaccg gccgctacaa
    12001 gcgcgaggat gcggtggcgt ttccgccgat tggtgttagc catcttgtcc cgctagcaga
    12061 gacgctcggt tgcgactcgg atgtagcttc ccgtcgcggc gtcgacatcg gccggcgtgg
    12121 gcggcatcga actcatgtga gcgtcgtgca cgtcgctgga caccagcgga tgcacgaagt
    12181 cccacaccgc ctgtggagtc tgccccgcag cacgggcctg gcacacgtac gagccgatgg
    12241 acaaggccag cagttcactc gacgggtgga cccctcccgc ggccagcgcg tccaggtagg
    12301 cgcgttgctg gccggtcacc gtcagtttgt tcaccgtctc tgacccgctg ctgccggcgg
    12361 gtggcggagc cggcgacgcg ccagggtcct ggcgacccga taccagcgcc gtggtggtgt
    12421 cgtcggcggg cagcgtgacg ctggacagca agccgtcatt cgccgagcac cctgacaaca
    12481 cagccgtggc aagcgccagc gtcgctaccg tcgagggccc gcgcatccgc gtggacgtcc
    12541 gttgcacgtc tccacggtac cggctcagtc gcaccgcacc gcgtgagcaa cgtcgcagtt
    12601 tgctcacgcg tcgggcacag ggactacttg atcgtcgcca ccaactcgcc ggccatcgcg
    12661 cccagttgcg gaccccacgt cgaccagtcg tgattaccgg agggcgggaa gtcgaagtgc
    12721 ccgttgtggc cgctgacctg gcggtagtgc tggtagaacg atcggttgct gccctgcgcc
    12781 tggtcacagt aggagatcat cgccgccggg tcgctgcacg tcagcgtctg cgggctgaac
    12841 acccacagcc gggtgttgtt ctgcgccagc aacgagatat gcacgtcggg gtcgtgccac
    12901 ttccagcgac ccagttgcgg caggccccac atgttgcgac tgtcgacgcc gccgaaccgc
    12961 atcagcccgt cggtgatcgc cccgttcatc gcggtggccg acggggtgag aaaaccagac
    13021 agcgatccgg cgaaccggaa ccggtcgggg tagaacgcgg ccagcgtgat cgcgccggtg
    13081 ccgccctggg cggcgccgac gatcccgtga ccgttggggg cgagtcccct gttcgcggcc
    13141 agccaatccg gcagctcttg ggacaggaag gtctcccact gccggctgcc gtcctgctcc
    13201 cagttggtgt agaggctgta cgcgccgcca gccggggcca ccaccgagat gccctggccg
    13261 gcgagcgtgt tcatcgcgtt gccggccgtc acccagttgc tgacgccgtc gcccgcgttg
    13321 aacgcgtcga gcaggaacac cgcgtgcggg ccgccgttgt agaacgcgac ggggatgtcg
    13381 cggcccatcg ctccggaggg gaccatcagc atttcgacgc tgtcggcctt cgcggtaccc
    13441 gacattgccg gagccgtcca cagggcaagc ccgagaatcg ccactagcag cgctcgcacc
    13501 agcaccacac cgaccttcat agcttcccct ctcggcatgc cgtccgccac gtgggtcctt
    13561 acccgaatcc gcaacgtagt gaaccacatc acgcagcaaa ggcgcgtatg aacggccgac
    13621 ggcggcgacc gtagtggtcg ccgccgtcgg ccgattgcgc agcgcctacg cggtgggcgt
    13681 ggcgcccaac acccgctgga tgtcgggctt catcgcgttc agctgcgcac cccagtagac
    13741 ccagctgtgc gtgcccgcct cggggaagtt gaacaccccg ttcgtgccgc cggccgcgat
    13801 gtaggcgtcc ttgaacgact tgttcgtccg aagggtgaag ccttcgagga acttggcgtt
    13861 gaagtcgccg ccgcccagct cgttgggctt gccgttgccg cagtacaccc agatgcgggt
    13921 gttattggcc accagcttgc tgacgttgac catcgggtcg ttgcgcttcc acgccggatc
    13981 gctggacggg ccccacatgt cctcggactt gtagccgccc gcgtcaccca tcgacatgcc
    14041 gacgagcatc ggccaccagc cttcggagag gttcaggaag ccggacagcg acgcggcgta
    14101 cgggaactgc tgcgggtagt agatcgccag agtcatagcc gccgagccgg ccatcgacag
    14161 gccgaccgcg gcgctgcccg tcggcttgac ctgcttgttg gccgccaggt acgcaggcag
    14221 ctccgaggtc aggaacgtct cccagttgta ggtgaggcag ccgctcttac cgcaggcttg
    14281 tttgtaccag ttgctgtaga agctggactg gccgccgacg ggcatgacca ccgacaaacc
    14341 ggactggtag tacatctcga acgcgttggt ctcgatgtcc cagccgctgt agtcgtcccg
    14401 cgcgcgcagg ccgtcgagca ggtagacggc cggggagttg gcgccgccgc tctggaactg
    14461 cacccggatg tcgcgtccca tcgacgccga cgggacgtcc aggtactcga ccggcagtcc
    14521 cggtcgggag aacgcccccg cagtcgccga gtcccccacg acgccgacca ggcccggcaa
    14581 cagcaccgcc gccatggcgg cgatcgtcag ccggcgcgcc catcggccgc gcaacttctc
    14641 aacgaacttc atatatgtgt ccatcccatc cttttgaaag cgcaacgacg gcgcccccgc
    14701 cgccgtgctc tggggtagtc aaccacaccg gggtgtggtc ctctcgacgt gagtccttgc
    14761 gatggtgcgg cccgccaggg ccgcgagtgg ttgatggggg ccggaaattg cgaggtcacg
    14821 ggctgcatcg gagcggctgg cgtggtcacc ggtagcccgc gcagccggag gcgcgcagcg
    14881 ttgaccggca acggcaacgt gctcagaatg tgttgctcaa aaaccattcg tcgatgtgaa
    14941 tctcgatgtg ggtatcgccg caccattgcg gttcgttacc acgatcgcag ccggccagct
    15001 aactcggcta gggccccgtc gccggtaaac cattgtaagg ggtaccacgt gagggcggct
    15061 cactcaagcc gcagcggatc agctcgtagc gcggcacccg gtcgattcgg tagctggtga
    15121 actgatgcgc atgcagcaga ttggacagga atcgccgcat cgtgagcggc cccctgatgg
    15181 acgtcagcac cgcgtcggtg gccgggcact tgagggcctc ttcggcttgc gtgacccagt
    15241 cctcgtcgag gtagggcggt aggtagggat gctccttcaa ccacgggccc tctgccaccg
    15301 cccagtccgg gaaaaggttc ttgtcgtggc cgatcctggc gtcctgcagc cgagcggtgt
    15361 gggccgccag cgggtttgct agaccgatct ggtcgaccac ccgaacatcc agtccgacgt
    15421 tcatgccgag catgcccaga ttggtgaaga acacggtatg cgggccgttc ttatccgctc
    15481 gttgtcccag ttccgcagga tccgcgggga cgacgtccca ctgcgtgtag ttgccggacg
    15541 gcagcagcag cgcaccgtcg ggggtgttct caatcgcttc gatggccgcc cgcatgcgcg
    15601 ggtagtcgag gtagtcggcg gccgtcagcg ggtgggcgtt gccggtggcc tgggcgtaga
    15661 accggcgctc gtcgacgatg ccggtatacg tgacccgggt ggcgtcggtg cccatgcccg
    15721 gcgagttcgc cgcccacagc gcccacaccg cgaccgcgag ccacaccgca gccgtcgcac
    15781 ccgcgagcac cacgcctctc cctcgcgaca gcttcatccg ctcgggcagc acgaccggga
    15841 tcaccgcaac cggagccagc acgcagaaca gcggcgtcag caaaacccga ccgtgcatga
    15901 agtcgccgcc ctgccggatc cagtagagcg cctgcagcag tccgctgacg acgatgaaca
    15961 ccaccaccgc ggtcgggctc tgcacgagc
//
LOCUS       DASWBF010000430         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_371755,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000430 DASWBF010000000
VERSION     DASWBF010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371755"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF874_15920"
     CDS             complement(<1..234)
                     /locus_tag="VF874_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HEX9177491.1"
                     /translation="MTTTTLSWDVVPIDKPDDANVVIGQAHFIKTVEDLHEAMVGVSS
                     SLRFGLAFCEASGPRLVRRTGNDPELVELATRHA"
     gene            complement(361..819)
                     /locus_tag="VF874_15925"
     CDS             complement(361..819)
                     /locus_tag="VF874_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HEX9177492.1"
                     /translation="MSTSDGAMESSAGEYSVDEDDQLQPEDTLVDRGVDDVLDEGYSP
                     PERPYGGGSFGSTPESLEQLLAEEEPDPAMRLYDPLDKDELQRSDEAERETEFPQRNE
                     VGRARAGRLVAPDQGFGEDVDANLLADDVGIDGGAASAEEAAVHVIDDEE"
     gene            complement(1016..1123)
                     /locus_tag="VF874_15930"
                     /pseudo
     CDS             complement(1016..1123)
                     /locus_tag="VF874_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1392..1745
                     /locus_tag="VF874_15935"
     CDS             1392..1745
                     /locus_tag="VF874_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177493.1"
                     /translation="MTRRLTAGRRLRRDLDRALDEAAAECGEVLVWTQTDLQMIGLMC
                     AAADRADALRDIWESSLASGVSASTLTKLSGEIRQCERQIAGLLDRLDVGLQGSWAKQ
                     QRKRASRSRGSIAIA"
     gene            complement(2085..3011)
                     /locus_tag="VF874_15940"
     CDS             complement(2085..3011)
                     /locus_tag="VF874_15940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005100517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9177494.1"
                     /translation="MSKAVRFDLYGDIDVLEVREVDRPVPGEGEVLVEVKAAGINPGE
                     AKIRTGVLHEIWPATFPSGQGSDLAGVVADVGPGVSTFAVGDEVLGFCESRSSHAEFV
                     AVPTEQLTAKPAQLSWEVAGSLFVAGTTAYAAVRAVRLTPNDTVAVAAAAGGVGTIAV
                     QLARRTGATVLGIAGPANDEWLTAHGIIPVNYGDDLAGRLRAASPSGRVDAFLDLFGG
                     GYVDLAVTGLGVAPERVDTIIDFPAVEKFGVQAAGNADAASAAVLAELAALAAAGDLE
                     VPIAGVFALDDVRAAYRTLEEQHTRGKLVLRP"
     gene            complement(3189..>3381)
                     /locus_tag="VF874_15945"
     CDS             complement(3189..>3381)
                     /locus_tag="VF874_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003916985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX9177495.1"
                     /translation="SGTVDAGERNDLIRRVRLGVLDYKRRAVTDLRDRNRIDDIVLRE
                     LQSAMDLEEVRLLGPADTE"
BASE COUNT          543 a         1150 c         1103 g          585 t
ORIGIN      
        1 cgcatggcgg gtcgcgagct cgaccaattc aggatcattg ccggtgcggc gaaccaaccg
       61 tggcccggag gcctcgcaga aggccagccc gaaccgtagc gacgagctca cccccaccat
      121 cgcctcatgc aggtcctcga ctgtcttgat gaagtgcgcc tgcccgatca cgacattggc
      181 atcgtccggc ttgtcgatgg gcaccacatc ccaggaaagc gttgtcgtgg tcacacgcac
      241 atgctgccac cggcacgcga cagccacgtc cgatgcttgg aatgcgaacg gtcctctttg
      301 cgggcgccgc gttcgcccgt gcttaggcag ccggatctaa aattcgtgag cgagggccag
      361 ctactcctca tcatcgatga cgtgaacggc ggcctcctcg gcggaggctg cgcctccgtc
      421 gataccgacg tcatcagcca acaggttcgc gtctacgtct tcgccaaacc cctggtcggg
      481 cgcgacaagt cgacctgccc gcgcgcgtcc cacctcattt cgctgcggaa actcggtctc
      541 ccgctcggct tcatcggagc gttgcagctc gtccttgtcc aacgggtcat atagccgcat
      601 tgccggatct ggctcttcct cggccagcag ctgctccaag ctttcaggcg tcgagccgaa
      661 ggagccacct ccgtacggcc gctcgggcgg cgaatatccc tcgtcgagca cgtcgtcgac
      721 gccacggtcg acgagggtgt cctctggctg aagttggtcg tcctcatcca cgctgtattc
      781 gccagccgac gattccatag cgccatccga ggtactcatg actcacacga tgtcactaca
      841 cccgggaagc gccatggtgg gggtgggtcg cgtggcaagg tcttcgcccg gttcggccac
      901 gaaccgttgt ctaacccgga cacggtgacg acaccagcct cggcgcggag cgtcccgcac
      961 ctggacgagc ggcacacggg gctggtgaca gaaaaaccag tggccaccgc gctcctgcca
     1021 ccagctcggc gccgccagcc ccctgctggt cgaactcgat gtcctcgatc accgtccgct
     1081 tatcgacacc caacagccca cgccataacc tcacattgcg cacgtcgttg cccgctcctt
     1141 tggttcctga ccttcaacaa gccagaaacc ttaagcgaca acgacgtgcg ccctctcaga
     1201 tcagcaaccc gcccacggaa gtgtcagaag agccgaattt tgccaaatag ccttgtgctt
     1261 cttcacgttt cgcggcgatc caatcggatt taacctgttg cagcgcaatg ggtttgcgac
     1321 gtccgccgat ggcgcggata tgcccgccgc tacccgcttt cgggccgttt gagcaggtag
     1381 aatcgcccgt tatgacccgc agattgaccg ctgggcgccg ccttcgccgt gatctggatc
     1441 gcgccttgga tgaggcggcg gccgagtgcg gcgaggtgtt ggtgtggacg cagacggacc
     1501 tgcagatgat cgggttgatg tgcgcggcgg cggatcgcgc tgatgcgctg cgcgacatct
     1561 gggagtcgtc gttggcatcg ggtgtgagcg cgtcaacgtt gacgaagctc agcggtgaga
     1621 ttcgccagtg cgagcggcag atcgccggtc tgttggaccg gttggatgtg gggttgcagg
     1681 ggtcgtgggc caagcagcag cgtaagcgcg ctagtcgcag ccgggggtcg atcgcgatcg
     1741 cctgaaggct cggggacacc gccgtcctcc ttacgcggcg gtgggtctcg cgggggtggc
     1801 cggggtggcg cctcaggctg gttgggttcc tgagtttggc tgccggctcg gccgcttccc
     1861 aaccgaccgc tgatcactgt gatgcgcggc ggcggatcgt gtcgagcata ccggcgcttc
     1921 ttggaccgcg ccgcccaagg agtggaatgg ttgatcacga caccgacgac gtcgtcgcac
     1981 cgtgcaccgg ctggcgttac gacgctcgtg gtgtggaaat acgctctggg acaacgatgg
     2041 tggctgggac ggggcgccgc tgtcgcagcc gggttagggc tgggctaggg ccgcaacacc
     2101 aacttgcccc gggtgtgctg ctcctccaag gtgcggtagg cagcccggac gtcgtcaagc
     2161 gcgaataccc cggcgatcgg cacctcgagg tcaccggccg cggccagagc ggccaactcg
     2221 gccagcacgg cggcgctggc cgcgtctgcg ttgcccgccg cctggacgcc gaacttctcc
     2281 acggcgggga agtcgatgat ggtgtcgacc cgctcgggcg cgaccccgag gccggtgacc
     2341 gccaggtcga cgtagccgcc gccgaacagg tctaagaacg catcgacgcg acccgacggc
     2401 gacgcggcgc gcagccggcc cgccagatcg tcaccgtagt tgaccgggat gatgccgtgg
     2461 gcggtgagcc attcgtcgtt ggccggtccg gcgatgccga ggaccgtcgc gcccgtgcgc
     2521 cgggcgagct gcacggcgat cgtgccgacg ccgcctgccg ccgcggccac ggcgacggtg
     2581 tcgttcggtg tgagcctgac cgcccgcacc gccgcgtagg ccgtggtgcc cgcgacgaac
     2641 aggctgcccg cgacctccca cgacagctgc gccggcttgg ccgtgagctg ttcggtcggc
     2701 accgcgacga attcggcgtg gctggagcgg ctttcgcaga agcccagcac ctcgtcgccg
     2761 actgcgaatg tcgacacgcc gggcccgacg tcggcgacca ctccggccaa gtcgctgccc
     2821 tgcccggacg ggaacgtggc gggccagatc tcgtgcagca cgcccgtgcg gatcttggcc
     2881 tcgccgggat tgatccccgc ggccttcacc tcgacgagca cctcgccctc accgggcacc
     2941 ggacggtcga cctcacgcac ctcgagcacg tcgatgtcgc cgtatagatc gaatcgcaca
     3001 gccttactca tggcctctac gacaagcacg ccacgcccac tattccccgc tgtgcacgac
     3061 gccgacgccc gcggtgattg actgacgttt taccccttcg gccggtcggc gaccaggcag
     3121 cgtgtgaagg cgtctcggcg cggcgagtaa ccggagggtc cgccgcatcg gctcggcggc
     3181 ggcgagcgtt actcggtgtc ggcagggccg agaagtcgca cttcctctag gtccatcgcc
     3241 gactgcagct cgcgaagaac gatgtcgtcg atgcggtttc ggtctcgcag gtcagtgacg
     3301 gcgcgacgct tgtagtcgag cacgccaaga cgcacccgcc gtatcagatc gttcctctcg
     3361 cccgcgtcga cggtccccga g
//
LOCUS       DASWBF010000431         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_702602,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000431 DASWBF010000000
VERSION     DASWBF010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702602"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..850
                     /locus_tag="VF874_15950"
     CDS             80..850
                     /locus_tag="VF874_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177496.1"
                     /translation="MNTIDLARPSGQAVVVGATGEVGAAIVARLRKAGVPVVAVARDG
                     DRLEQLSATDDGITACPADIGDDSSGEAIAAAVSGPVRMVVQSAAVPPAGPLATIEPE
                     VLGRSVSLKLGGLLRLIRAVDDRFVQGSRIVAIGGHFGSEPTPQTCGAGVTNAALANL
                     IRQLADAYGPRGVTVHMIAPGVLYTDRLRRFAASHAEARGVSVETVLEEYRSHSPLGR
                     LTTPDQIGWAVTQLLAPEADALHGATLALDGGARRGLF"
     gene            946..1719
                     /locus_tag="VF874_15955"
     CDS             946..1719
                     /locus_tag="VF874_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9177497.1"
                     /translation="MQTIDLAYVERGVHEELVAQVADQEGYYEDEGVHVAIRNGVSWD
                     PERLRRGASIGLGRALLQRLTGGIGWKVLSVNTHRPLFWFLGNADVASMADLRGRRVA
                     VHPAQTAPGWFTRIVLREHALDPDRDLECVVRLPGDYQMDLRRLRDGSIDAACVGTTL
                     SPEQVAEEEGFHVLAWVGDHIQIPTVGVAVDPVHIPLDNPALQALVRANNRALRTIAE
                     QPDLAVDYIASFLNRLTRDEALAVAAELASSLFQVDLIL"
     gene            1895..3151
                     /locus_tag="VF874_15960"
     CDS             1895..3151
                     /locus_tag="VF874_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEX9177498.1"
                     /translation="MDESGLFTAALGLTPPWRVSRVEFDLEQTRLDLYLDFPRGARFA
                     CPAKGCGRRECPVHDTAAKTWRHMDFFQHKAFLHARLPRVRCPVHGVRQVGAPWAREG
                     SGFTLLFEALLLQFAAAMPVRKVAEMTREQDTRIWRVLHHHVDAARACADYSRVDRVG
                     MDETAAARGQDYVSIFADMTCAKVLYATEGRDGDTVARFAADLRDHGGYPEAVCDTSS
                     DMSAAYIAGIGEHLPNAAMTFDRFHVAAKLSEAVDAVRRDEVASRPELKRTRWLWLKN
                     HSNLTDTQQAELYRLTRPSARLATARALRWREDFQAFYDQPDDYADEYLRRWCYGAKR
                     SRLQPIKDFVATVERHWDGIVAWHASRLNNGLLEGINSLVQAAKARARGYRSKRNMIT
                     MIYLIAGDFTLPTVANPALAYPSAAA"
BASE COUNT          631 a         1260 c         1266 g          644 t
ORIGIN      
        1 ctatcttccg gtcaatggcg gcctcagtat ggattgaacg ggacagcgac gcatcagatt
       61 cggaaggaag acagtcccca tgaacacgat cgacctggca cgcccgagtg ggcaagccgt
      121 tgtcgtcgga gcgaccggcg aggtcggcgc tgccatcgtc gcgcggctac gcaaagccgg
      181 agtgccggtg gtcgcagtgg cccgcgacgg tgaccgcctc gaacagttga gtgcaaccga
      241 cgatgggatt acggcatgcc ccgcggacat cggcgatgac tcgtcgggcg aggcgatcgc
      301 cgccgcagtg tccgggcctg tccggatggt ggtccagtca gcagccgttc cgccggccgg
      361 accgctggcc acgatcgagc cagaggtcct tggccggtcg gtgtcgttga agctgggtgg
      421 gctgctgcgt ctcatccgcg ctgttgacga tcggtttgtc caggggtccc ggatcgtcgc
      481 catcggtggc catttcggtt ccgaacccac gcctcaaacc tgcggggccg gtgtcaccaa
      541 cgctgccctg gccaacctca ttcgccagct cgccgacgcc tacgggccac ggggggtgac
      601 cgtgcacatg attgctccgg gtgtcttgta caccgaccgc ctccgtcggt tcgccgcctc
      661 ccacgccgag gcgcgcggcg tgagcgtcga gaccgtcctc gaggaatacc gatcccactc
      721 accgctgggt cggctgacca cgcccgacca gatcggctgg gcagtaactc aattgctggc
      781 ccccgaggcc gacgcgctgc acggcgcaac tctcgccttg gacggcggcg cccgccgagg
      841 cctcttctag cccgttccag tcgggccgct caacggcggc cttggggtgg attgacatca
      901 ggttccgcgg ctacggaagt catcagattg ggaaggggtt tggccgtgca gacaatcgat
      961 ctggcgtatg tcgagcgtgg tgtccacgag gaactggtcg cccaagttgc cgaccaggag
     1021 ggctattacg aggatgaagg tgtccacgtc gccatccgca atggggtgag ctgggacccg
     1081 gagcgtctgc gccgcggcgc gtccattggt ctgggccggg ccctattgca gcggctgacg
     1141 ggcggtatcg gctggaaggt gctcagcgtc aacacccatc gtcccctgtt ctggtttctc
     1201 ggcaacgccg acgtggcatc catggccgat ctgcgcggtc gccgcgtggc ggttcacccc
     1261 gcccaaaccg cccccggttg gttcacccga atcgtcctgc gcgagcacgc ccttgacccc
     1321 gaccgcgacc tggaatgtgt ggtgcggctt ccgggtgact accagatgga cctgcggcgg
     1381 cttagggacg gctcgatcga cgctgcctgc gtcggcacca cgctctcccc cgaacaggtc
     1441 gccgaggagg aagggtttca cgtgctggcc tgggtcggcg atcacatcca gatccccacc
     1501 gtgggcgtcg cggtggaccc tgttcatatc ccgctggaca accctgcgct gcaggcgctg
     1561 gtgcgggcca acaaccgggc tctgcggacg atcgccgaac agcccgactt ggccgtggac
     1621 tacatcgcct cgttcctcaa tcgactgacg cgtgatgaag cgctcgcggt cgcggccgaa
     1681 ctagcctcct cgctgtttca agtggacttg atcttgtgat gttgtttcgc tgaggccgtg
     1741 ttgggtggtt cggtggcaag ccgacccgca ccgccggcgc cccggcgccg aggagcggaa
     1801 gcggcgcggc agccctgctc gcggttgacc ggcgcgccgg tcgcgtcgtt gactgggtga
     1861 ccgtcgttga cgttatcgca ggcgaggaga tcgcatggac gagtccggat tgttcaccgc
     1921 ggcactgggt ttgacaccgc catggcgggt gagtcgggtg gagttcgacc tggagcagac
     1981 gcggctggac ctgtacctgg actttccgcg gggtgcccgg ttcgcgtgcc cggccaaggg
     2041 ctgcggccgg cgcgagtgtc cggtccatga caccgccgcc aagacgtggc ggcacatgga
     2101 tttcttccag cacaaggcgt tcctacacgc tcggctgccg cgggtgcgct gccccgtgca
     2161 cggggtgcgc caggtcgggg cgccgtgggc gcgggaggga tcggggttca ccctgctgtt
     2221 cgaggcgctg ctgctgcagt tcgcggcggc gatgccggtg cgcaaggtgg ccgagatgac
     2281 tcgtgagcag gacacccgga tctggcgggt gctgcaccac cacgtggacg cggcccgcgc
     2341 ctgcgcggac tactcgcggg tcgacagggt cggcatggac gagaccgccg ccgcccgcgg
     2401 tcaggactac gtgagcatct tcgccgacat gacctgcgcg aaggtgctct acgccaccga
     2461 aggccgcgac ggagacacgg tggcccgctt cgccgccgac ctacgcgacc acggcggcta
     2521 cccggaggcc gtgtgcgaca ccagctccga catgagcgcc gcctacatcg ccgggatcgg
     2581 cgagcacctg ccgaacgcgg cgatgacctt cgaccgcttc cacgtcgcgg cgaaactgtc
     2641 tgaggccgtc gacgcggtgc gccgcgacga ggtcgcctcc cgccccgagc tcaaacgcac
     2701 ccgctggctg tggctgaaga accattcgaa tctgaccgac acccaacagg ccgagctgta
     2761 tcggctgacc cgcccctcgg cgcggctggc caccgcccgg gcgctgcgat ggcgcgagga
     2821 cttccaggcc ttctacgacc agcccgacga ctacgccgac gagtatctgc ggcgctggtg
     2881 ctacggcgcg aaacgctcac ggctgcaacc gatcaaggac ttcgtcgcga ccgtggagcg
     2941 gcactgggac ggcatcgtcg cgtggcacgc cagcagactg aacaacggcc tgctcgaagg
     3001 gatcaactcc ctcgtgcaag ccgccaaggc ccgcgcccgc ggctaccgca gcaagcgcaa
     3061 catgatcacc atgatctacc tcatcgccgg cgacttcacc ctgcccaccg tcgccaaccc
     3121 cgccctcgcg tacccatccg ctgccgcctg aacagaccac ttaagccaag cctaaccaac
     3181 ccacttgaaa cagcggagaa cctcgccagg gcgtccgcaa ttttgacgtt gaatggggcc
     3241 agcgtgattt cgacaccgaa gatttttagg ccgcccagtc gctcccacag gaacgccacc
     3301 gccagcggcc cagccgcaag taagccgacg atgacagccc gatgctcagg aagatttggc
     3361 cacactaagt gtgcgacgcc gtaagcgacg gcgaataccg cgacaatcga gtacagaacg
     3421 agcgcggtca ttccgtaccg tcgtccgatc cggcgaagca tcccaataga ccgcctaaac
     3481 gtcgaagata cgtccattga tccgctctcc aaagtcagac gtagtggtgt aagagactca
     3541 gcagtgggcc ggggctcggc gttgagcgta ggcgttttcc acctcgacgc gcgaaataat
     3601 tgcagcgcag ccgcctagga cgacgctgaa taactcgtcc aatgctggtc gtgtctgacg
     3661 gttgtgtgcc ctgtggcgtg tgatgatgtt gtggtgcaag ggattacgcg gtcggatcgg
     3721 gagttgttgg acgcgggcgc gctggtcggg gagctggtgc cgccgggcag tgtgttcgcg
     3781 tttttggccg agcaccggca c
//
LOCUS       DASWBF010000432         4933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_436131,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000432 DASWBF010000000
VERSION     DASWBF010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436131"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VF874_15965"
     CDS             <1..367
                     /locus_tag="VF874_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177499.1"
                     /translation="LVAKLRYQQTKIDGAPDTILVPAEVVAIIGEQQQWAQRFFAAHG
                     APDKTPKYLFLALQMNRNGDRPYSGNTLRNLLTDLAVRLDVRDRTGALVDFNRTHRFR
                     HTVVICTAKEPVSHVSLAA"
     gene            425..1546
                     /locus_tag="VF874_15970"
     CDS             425..1546
                     /locus_tag="VF874_15970"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9177500.1"
                     /translation="MIAFRAERAVSPLDGSELWVVLDCGFVMHREASDFLRALHGAGR
                     SPQTIRAYAGRVASFLGWCADQGVQWSSIGLPGLARFKHFVEATPHRGGRLRSGTTVN
                     AILTAVCEFLRFCARTGVIDQAVADRLSEPRWLRFTPPGFDAGESGQFRTMRTRTLKA
                     RAEAAFPEAFTIEQAERVVECCRRPRERFMVTLLRDGGLRIGEALGLRRSDLHLLPDS
                     RRVGCTVLGAHVHVRHRANPNGALAKSRFPRTVPASQAVLAAYADYQFERTEILGGDD
                     CDMVFVNLYHEPLGAPMTYRAAKRFFERLATECGFAVRPHMLRHTAATNWVRAGVDLD
                     VVQRLLGHASLASTAVYLHARDEDKRRAVEAVAAGERFR"
     gene            1543..4017
                     /locus_tag="VF874_15975"
     CDS             1543..4017
                     /locus_tag="VF874_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9177501.1"
                     /translation="MSTPASHLLVGTGADPRSVTDAGWLQWLSAHIDAAWRPDEWDGS
                     LWLFTGDLDSERTAAWRCRTPGCPTPARGFNGRCASCRRSRSTAGVSEEEFDRAPRRR
                     PTRPVARGGCTVPDCQGELHCRGLCFRHERAWRGAGGGPVEQFIAQARPLTRSEPCLV
                     TGCGRQRVTRRGLCHFHRNRLQRQRNSASMSPQELAVWVAGEKPRIAAHQFSLAGLPE
                     LVRLELLYALQRRDEAPPPLDPLPVRILIARLRGADSVRHVDPETVCGSGGMQYNAAV
                     TGLFADLRRYLERAWTQYAGADPYAGDVWQVQLLDLQSNGSRRWPATKGTVDFGPIEL
                     RWLREVIKDWARNTRPYLQRLRQALRACRVASQTLVACGRADQTSLGAGDFVLVERAI
                     VEQRRTDGSPHSASYRTQLLRVFCEVIEHGRANALMTEVPDPFRPSPRRQRVVADVNE
                     EQLGKALPDTVIRQLDQHLHLLGPAGRHGSISAADLRAMHQTIYQILRDTGRRPGEIV
                     SLKIGCVEIIDGQHNLIYDNHKAARRRRRLPITADTAEVIKTWQHRREQMPTAPATRP
                     WLFPSPLLRSRQARGHLTASCVGVAFRAWTRSIPKIDGELLDPDGTPLPFDRSLITPY
                     VLRHCYAQRHADAGVPVDVLKDLLDHSSIQTTMGYYSITHKRKQQAIRAVGSLAVDAE
                     GNPSSFADPLAYERASVSVPFGNCTEPSNVKAGGGACPIRFQCAGCGFYRPDPSYLPA
                     LEEHIHSLKADRETAQAMGAADYVIANMSSEIDAFTRVVDTMRSRLADLDPQQRAEVE
                     ESSRILRRARAARRLPLIATTAKQTG"
     gene            4014..4604
                     /locus_tag="VF874_15980"
     CDS             4014..4604
                     /locus_tag="VF874_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177502.1"
                     /translation="MSTTTANQARAAALKAARAKDSDVKRRRTLAALQTLEATGTAIT
                     FTAVAEAAGVSTWLVYADGIREHIDAARNRQNNQHNGTGTPTAPSRQPATQDSLRTDL
                     AIAREQIKTLRSERDKLQQRLRLQLGAELEAPDRAHLTARVTDLETINRQLVAERDAR
                     TIEADAAKRRVTELEDELSAARESLRRVIKAQNRAP"
     gene            4679..>4933
                     /locus_tag="VF874_15985"
     CDS             4679..>4933
                     /locus_tag="VF874_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177503.1"
                     /translation="MASIFTEKVQITAAMRLLHAGVDTSVIALWLGHYAGDLVKRFLS
                     GGCVVEAGEQSVEDLFPPDLALGVRVVALSLQGGAEVDGGL"
BASE COUNT          807 a         1712 c         1604 g          810 t
ORIGIN      
        1 actggtggcc aagctgcgct accagcagac caagatcgac ggtgcgcccg acaccatcct
       61 cgtacccgcc gaggtcgtcg cgatcatcgg cgaacaacaa caatgggcgc agcggttctt
      121 cgccgcgcac ggcgcacccg acaagacccc gaagtacctg ttcctcgctt tgcagatgaa
      181 ccgcaacggc gaccggccct attccggcaa caccctgcgc aacctgctga ccgatctggc
      241 cgtccggctc gacgtgcgtg accgcaccgg cgccctggtc gacttcaacc gcacccaccg
      301 gttccgccac accgtggtta tctgtactgc caaggagcct gtgagccacg tgagtttagc
      361 ggcgtgacac gccggtgcct tacgaatatc cggtactttc aggcacggcc gttacgtttg
      421 gcacgtgatc gcgttccgcg ccgaacgggc ggtttcaccc ctggatggat ccgagttgtg
      481 ggtggtgctg gactgcgggt tcgtgatgca ccgggaggcc agcgatttcc tgcgagcgct
      541 gcatggggcc gggcgttcgc cgcagacgat ccgtgcctat gccgggcggg tggcgagctt
      601 tttgggctgg tgcgccgacc agggggtgca gtggtcctcg atcggcctgc cgggcttggc
      661 ccgattcaag cacttcgtcg aagccacccc gcaccggggc gggcgattgc gttcgggcac
      721 aacggtaaac gcgatcctca ccgcagtgtg cgagttcctg cggttttgcg ccaggaccgg
      781 ggtgatcgac caggctgtgg ctgaccggtt gtctgaaccg cgttggctgc ggttcacccc
      841 gccaggtttt gatgccggtg aatccggcca gttccggacg atgcggaccc gcacgctcaa
      901 agcgagggcg gaggcggcgt tcccggaggc gttcaccatc gagcaggccg aacgggtcgt
      961 ggagtgttgt cgcaggcctc gggaacgatt catggtgacc ctgcttcgtg acgggggcct
     1021 acggatcggg gaagctctgg gattgcgccg atccgatctg cacctgctgc ccgattcgcg
     1081 gcgggtgggt tgcacggttt tgggcgctca cgttcacgtg cggcaccggg ccaatcccaa
     1141 tggcgcgctg gcgaagtcgc ggtttccgcg cactgtgccg gcgagccagg cggtgctggc
     1201 ggcgtatgcg gactaccagt tcgaacgcac ggagatcctc ggcggcgacg actgcgacat
     1261 ggtgtttgtc aacctctacc acgaaccgct gggcgcgccg atgacttacc gtgcggcgaa
     1321 acggttcttc gagcggctgg ccacagagtg cggctttgcg gtacggccgc acatgttgcg
     1381 gcatacggcg gctacaaact gggtgcgtgc cggggtggat cttgatgtcg tgcaacggct
     1441 tttgggtcac gcatcgttgg cctcgaccgc tgtttacctg catgcgcgag atgaggacaa
     1501 acggcgagcc gtggaggctg tggccgccgg ggagcgcttt cgatgagtac gccggcgtct
     1561 cacctgctgg tcggcaccgg cgccgacccg aggtcggtga ccgatgctgg ctggctgcag
     1621 tggctgtctg ctcatatcga tgccgcgtgg cggccggacg agtgggatgg gtcgctgtgg
     1681 ctgttcaccg gcgatttgga cagtgagcgg accgcggcgt ggcggtgccg gactccgggc
     1741 tgccccactc ccgcccgggg ctttaatggc cgctgcgcga gttgccgacg ctcccggtcg
     1801 acggccggtg tctccgagga ggagttcgac cgtgctcccc ggcggcgccc gacccgcccg
     1861 gttgcccgcg gcggctgcac ggtgcccgac tgtcagggcg agttgcattg ccgcggcctg
     1921 tgtttccggc acgagcgggc ctggcgcggc gccggtggcg ggccggtgga gcagttcatc
     1981 gcacaggctc ggccgctgac ccgcagcgaa ccgtgcctgg tcaccggttg cggccgtcaa
     2041 cgcgtcaccc ggcgcgggct gtgccacttc caccgcaatc ggctgcagcg acaacgcaac
     2101 tccgcgtcga tgtcgccgca ggagctggcc gtgtgggtgg ccggtgagaa gccccggatc
     2161 gcggcccacc agttctcgct tgccggtctg cccgagctgg tgcgtttgga gctgctctac
     2221 gcgctgcaac gccgcgatga ggcacctcct ccgctggacc cgttaccggt tcggatcctc
     2281 atcgcacgcc tgagaggggc cgactcggtt cgccacgtcg accccgaaac cgtgtgtggc
     2341 agcgggggta tgcaatacaa cgcggccgtc acgggcttgt tcgccgacct gcgtcgttat
     2401 ctggaacgag cctggaccca gtacgccggc gctgatccgt atgccggcga cgtgtggcag
     2461 gtgcagctgc tggacctgca gtccaacggc tcacgccgct ggccggccac caaggggacc
     2521 gtcgacttcg gcccaatcga gctgcgctgg ctgcgcgagg tgatcaagga ctgggcccgt
     2581 aacacccggc cctacctgca acggctgcgg caagcgctgc gggcctgccg cgtggcttcc
     2641 caaaccctgg tggcctgcgg ccgcgccgac cagaccagtc tgggggctgg cgacttcgtt
     2701 ctcgtcgagc gggccatcgt cgagcaacgc cgcaccgacg gcagtcctca ctccgcctca
     2761 taccgcaccc agctgctgcg ggtgttctgc gaggtgatcg agcacggccg cgccaacgcg
     2821 ctcatgaccg aggtgcccga cccgtttcga ccgtcgccgc gtcgtcaacg ggtggtcgcg
     2881 gacgtcaacg aggaacagct gggtaaggcg ttgcccgaca ccgtgattcg ccagctcgac
     2941 cagcaccttc acctgttggg tccggcgggc cgtcacgggt cgatcagtgc tgccgacttg
     3001 cgggcaatgc atcaaaccat ctaccagatc ctgcgagaca ccggccgccg ccccggcgag
     3061 atcgtcagcc tcaagatcgg ctgcgtcgaa atcatcgacg gccaacacaa cctgatctac
     3121 gacaaccaca aggccgcccg gcggcgtcgc cggctgccca tcaccgccga caccgccgag
     3181 gtcatcaaga cctggcagca tcgccgcgag caaatgccca cggccccggc gacccggccg
     3241 tggctgtttc cctccccgct gctgcgctcc cgccaggccc gcggccacct gaccgcttcc
     3301 tgcgtcggcg tggcgtttcg ggcttggacg cggtcgatcc cgaagatcga cggcgaacta
     3361 ctggaccctg acgggacacc gctgccgttc gaccgctcgc tgatcacccc gtacgtgctg
     3421 cggcactgct acgcccagcg ccacgctgat gccggcgtgc ccgtcgacgt gctcaaagac
     3481 ctgctggatc attcttctat ccaaaccacg atggggtact actcgatcac ccataagcgc
     3541 aagcagcagg ccatccgcgc tgtcgggtcc ctggccgtgg acgccgaggg caacccgtcg
     3601 tcgttcgccg acccgctggc ctacgagcgg gcctcggtgt cggtgccgtt cggcaactgc
     3661 accgagccgt ccaacgtgaa ggctggcggc ggagcctgcc ccatccgatt ccaatgcgcc
     3721 ggttgcggct tctaccgccc ggacccctcg tatcttcccg ccctcgaaga gcacatccac
     3781 tcgcttaagg ccgaccggga gacggcccag gcaatgggcg ccgccgacta cgtgatcgcc
     3841 aacatgagca gcgagatcga cgcgttcacc cgcgtggtcg acaccatgcg gagccgtctc
     3901 gccgacctcg acccccagca gcgggccgag gtcgaagagt ccagccgtat tctgcggcga
     3961 gcccgcgccg cccgccgcct tccgctcatc gccaccaccg ccaagcagac cggatgagca
     4021 cgaccacggc caaccaagcg cgtgccgcag cactgaaagc ggcccgggcc aaagacagtg
     4081 acgtcaagcg tcgacggacc ctggccgcgc tccaaaccct ggaagccacc ggtacggcga
     4141 tcaccttcac cgcggtagcg gaggccgccg gggtctccac ctggctggtc tacgccgacg
     4201 gaatccgcga acacatcgac gccgctcgaa accgccagaa caaccagcac aacgggaccg
     4261 gcacaccgac ggctcccagc agacaaccgg cgacgcagga cagcctgcgc accgacctgg
     4321 cgatcgcccg cgagcagatc aagacgctgc gctcagaacg cgacaaactc cagcagcgcc
     4381 tgcggctgca actcggcgcc gaactcgaag cacccgaccg cgctcacctg accgcacggg
     4441 tcaccgacct cgagaccatc aaccgtcaac tcgtcgccga acgcgacgcg cgcaccatcg
     4501 aggccgatgc cgccaaacgt cgggtcaccg aactcgaaga cgaactatcc gctgcacgcg
     4561 aaagcctgcg tcgggtcatc aaagcccaaa accgcgcacc atgacgccta cctccgcagc
     4621 ggctcccgcc cgcagtcacc cccgacgtgc ctcacgtgcc gagcactcga aaacgcctat
     4681 ggccagcatt tttacagaaa aagtacagat aaccgcggcg atgcggctcc tgcacgccgg
     4741 ggtcgacacc agcgtcatcg cgctctggct tggtcattat gcgggtgatc ttgtcaagcg
     4801 gttcttgtca ggaggctgcg tcgtcgaggc gggagagcag tccgtcgaag atctttttcc
     4861 gcccgacctg gcccttggtg tccgcgtcgt cgcgttgtcg ttgcagggtg gggcggaagt
     4921 cgatggtggt ctg
//
LOCUS       DASWBF010000433         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1297760,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000433 DASWBF010000000
VERSION     DASWBF010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297760"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VF874_15990"
     CDS             complement(<1..609)
                     /locus_tag="VF874_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015385292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9177504.1"
                     /translation="MGARIAVVGPGAVGATVAAYLHNAGRPVVLCGRTRRDQLDVRPD
                     GGDRILVPGPVHTDAGDVKGPVGLVVLAVKATQLEAAAKWLAVLCDEHTTVCVLQNGV
                     EQVELVQPHCPRSTVVPGIVWFGAETQPGGWVRLRGEPRLTLPASAVYVADALREAGC
                     VVTAATDFTTEAWRKLLVNALAGFMVLAGRRSGMFRRDDIAAL"
     gene            681..>1793
                     /gene="aspS"
                     /locus_tag="VF874_15995"
     CDS             681..>1793
                     /gene="aspS"
                     /locus_tag="VF874_15995"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX9177505.1"
                     /translation="MLRTHAAGSLRTADAGQKVTLAGWVARRRDHGGVIFIDLRDASG
                     VSQVVFREGDVLEQAHRLRAEFCIAVQGVVEIRPEGNANPEIATGDVEVNATSLTVLG
                     ESAPLPFQFDEPAGEEARLKYRYLDLRREGPGGVIRLRSKVNAAARAVLAGHDFVEIE
                     TPTLTRSTPEGARDFVVPARLQPGTFYALPQSPQLFKQLLMVAGMERYYQIARCYRDE
                     DFRADRQPEFTQLDVEMSFVEAEDVIAISEEVLASLWALVGYELPGPLPRITYAEAMR
                     RFGSDKPDMRFGLELVECAEFFRDTPFRVFQAPYVGAVVMPGGASQPRRTLDGWQDWA
                     KQRGHRGLAYVLVGDDGTLSGPVAKNLSDAERDGLAA"
BASE COUNT          273 a          633 c          625 g          262 t
ORIGIN      
        1 cagcgccgcg atgtcgtcgc gccggaacat ccccgaccgg cgtccggcga gcaccatgaa
       61 cccggccagc gcgttgacca gcagcttgcg ccacgcctcg gtggtgaagt ccgtcgcggc
      121 ggtgacgacg cagccggcct cgcgcagagc atcggcgacg tacacggcgg aggccggcag
      181 cgtcagccgc ggctcgccgc gcagccggac ccaaccgccg ggctgcgtct cggcgccgaa
      241 ccacacgatg cccggcacga ccgttgatcg cgggcagtgc ggctgcacca gctcgacctg
      301 ttccacaccg ttctgcagca cgcacaccgt ggtgtgctcg tcacagagca cggccagcca
      361 cttcgcggcc gcctcgagct gggtcgcctt gacggccaac acgacgaggc cgacggggcc
      421 tttcacgtcg ccggcatcgg tgtgcaccgg gccgggaacc agaatccgat cgcccccgtc
      481 gggccggacg tccagctggt cccgacgcgt ccggccgcat agtacgaccg gacgcccggc
      541 gttgtgcaga tacgccgcga ccgtcgctcc gacggcgccg ggacccacca ccgcgatgcg
      601 tgcgcccacc cgcgccacgc taccggcgca ttcggcacat ttagactggg ttgtcatccc
      661 gatccaggca ggagtctttt gtgctgcgca cccacgccgc aggttcgctg cggaccgccg
      721 acgccggtca gaaggtgacg ttggcgggct gggttgcgcg tcggcgcgac cacggcggcg
      781 tcatcttcat cgacctgcgc gacgcgtccg gagtgtcgca ggtggtgttc cgtgagggcg
      841 acgtgctcga gcaggcgcac cggctgcgcg ccgaattctg catcgccgtg cagggtgtcg
      901 tcgagatccg gcccgaaggc aacgcgaacc ccgaaatcgc caccggcgac gtggaagtca
      961 acgccacctc gctgaccgtc ctcggcgaaa gtgcgccgct gccgttccag ttcgacgagc
     1021 ccgcggggga ggaggcgcgg ctgaaatacc gctacctcga cctgcgccgc gagggacccg
     1081 gtggggtaat tcggttgcgc tccaaggtga atgcggccgc acgggcagtg ctggccggcc
     1141 acgatttcgt cgagatcgaa accccgacgc tcacgcggtc caccccggaa ggcgcgcgtg
     1201 acttcgtggt gcccgccagg ctgcagcccg gcacgttcta cgcgctgccg cagagcccgc
     1261 agttgttcaa gcagctgctg atggtggcgg gcatggagcg ctactaccag atcgcccgct
     1321 gctaccgcga cgaagacttc cgcgccgacc gccaacccga gttcacgcag ctcgacgtgg
     1381 agatgagctt cgtcgaggcc gaggacgtga tcgcgatctc ggaggaggtc ctcgcgtcgt
     1441 tgtgggcact ggtcggctac gagctgcccg ggccgctgcc gcgcatcacc tacgccgagg
     1501 cgatgcgccg gttcggttcc gacaagccgg acatgcggtt cgggctggag ctggtggaat
     1561 gcgcagagtt cttccgcgac acgccttttc gggttttcca ggcaccatac gtcggcgccg
     1621 tcgtgatgcc cgggggcgcg tcgcagccgc ggcgcacgct ggacggctgg caggactggg
     1681 ccaagcagcg tgggcaccgc gggctcgcgt acgtgctcgt cggcgacgac ggcacgctga
     1741 gcgggccggt ggccaagaac ctttcggacg ccgaacgtga cgggctggcc gcc
//
LOCUS       DASWBF010000434         4760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1229914,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000434 DASWBF010000000
VERSION     DASWBF010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229914"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF874_16000"
     CDS             complement(<1..318)
                     /locus_tag="VF874_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEX9177506.1"
                     /translation="MAAVTSVVENEVALVQEWRDKKRYLWLTGLVAPTALFVVLPIIW
                     AMNQQSWHTASQVLLWIGPLLLFVLLPLLDLKFGADGQNPPDEVMERLENDKYYRYCT
                     YAFI"
     gene            485..1336
                     /locus_tag="VF874_16005"
     CDS             485..1336
                     /locus_tag="VF874_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016894496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX9177507.1"
                     /translation="MPRMDRRRMMLMTGIGVMAAAIPVPEAQAYPSGQNVPADRVPPP
                     AAPPGGQAGPYLFHDEFDGPAGSAPDPSKWLVSRARESIKDPTYWELPENVGQYRDDR
                     RNVFLDGKSNLVIRAAKDGSTYYGGKVFSPWQGGIGRTWEARIKLNCLTAGCWPAYWL
                     SSDAQGEIDIVEWYGNGNWASGTTVHARANGSEWATHNLALDSGWHTWRVQWDEAGMR
                     FWKDYVDGAQPYFNVPANSLADWPFNDPGYQVFPILNLAVAGSGGGDPGPGTYPADML
                     VDWIRVW"
     gene            complement(1391..1483)
                     /locus_tag="VF874_16010"
                     /pseudo
     CDS             complement(1391..1483)
                     /locus_tag="VF874_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1569..2960
                     /locus_tag="VF874_16015"
     CDS             1569..2960
                     /locus_tag="VF874_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX9177508.1"
                     /translation="MGRRGKHAVVIGASIAGLCAGRVLSDFYDRVTLFDRDDLPEHPA
                     NRSAVPQGRHVHILMSRGAQEFGALFPGLLDDMVAAGVPILEDQPGSIHFGAAGHVLG
                     IPTELEETFTTYVPSRPRLEWQIRRSTVWLPNVEIRQRSVSAPRFDAATQRVTGVVLD
                     DGDVVEADLVVDATGRGTRLPVWLSEWGFDRPVEDTVDVGIGYATQQLRIPDGLIEEK
                     VVVAGASREQPVGIGMLYYEDATWGMTTFGVGKVEPPHTFDEMCVVADAVLPQHAAAI
                     RQAEPIGEVAFHKYPTSRWRRYDKLTRFPAGIVPFGDAVVSFNPTYGQGMTMTSLQAG
                     HLHRALESADDDIARQLNRATAKTTYPVWTMNAIGDAMFHNATGDMPRWYRPVGNLFE
                     QFLGAAETEPVLAEWFLRRFSLLDSLYMIPSARIVGRTVRHNMRCWLAERRQTRERQP
                     DFAVTERSKSGSR"
     gene            complement(2912..3841)
                     /locus_tag="VF874_16020"
     CDS             complement(2912..3841)
                     /locus_tag="VF874_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9177509.1"
                     /translation="MSTEGSTKAILAALAANAGIAVAKFVGFLITRSSSMLAESVHSV
                     ADTSNQALLLLGQRRARKEADQLHPFGYGRSRYFYSFVVALVLFSLGSLFALYEGYHK
                     ISHPEPLKSPLVAIVILVLAIALESFSLRTAIVESRPLKGSGSWWRFIRTSRNPELPV
                     VLLEDLGALIGLLFAFIGVGLTIVTDDAVWDGIGTVAIGVLLGAIAAILMVETHSLLI
                     GEGATGAQDRAIREAIEQTAHVDRLIHIRTQYLGPEEMLVGAKVAVAPDIDLPAVAAV
                     IDDAEARIRAAVPAARVIYLEPDLDRSVTAKSG"
     gene            complement(3855..>4760)
                     /gene="manA"
                     /locus_tag="VF874_16025"
     CDS             complement(3855..>4760)
                     /gene="manA"
                     /locus_tag="VF874_16025"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEX9177510.1"
                     /translation="GFDREERRGIPVTSPTRNYRDRSHKPELVIALEQFEALAGFRGV
                     PRTIELMEALAVADLDPFVELLKGQPDADGLRVLFTTWITAPQPHLDVLVPAVLDGAI
                     HYVRSGAADFTDEAKAVLELGERYPGDAGVLAALLLNRMHMKPGEAMFLPAGNLHTYL
                     RGVALEVMANSDNVLRGGLTPKHVDVPELLRILDFHPVAEASLRPEMRCEGLELVYGA
                     PTEEFAVSRLRIDGEDLGHEIDAAVRHDGPQVLLCTQGAVQLHAKSSSLTLERGQAAW
                     VPADDGPIRLVAERPAELFRVSTGL"
BASE COUNT          833 a         1582 c         1526 g          819 t
ORIGIN      
        1 gatgaacgcg tacgtgcagt agcggtagta cttgtcgttc tcgagccgct ccatcacctc
       61 gtcgggcggg ttctgcccgt cggcgccgaa cttcaggtcg agcagcggca gcagcacgaa
      121 cagcagcagc ggaccgatcc atagcagcac ctgcgacgcg gtatgccagc tctgttggtt
      181 catcgcccag atgatcggca gcacgacgaa cagcgccgtc ggggcgacta gccccgtcag
      241 ccacaggtat cgcttcttgt cacgccactc ctgaacgagg gccacttcgt tctcgactac
      301 cgacgtcact gccgcaatcc tcccgtgaga cgtacatcac ctgtcgggtt tacgttagat
      361 aacgatttgt ctgttgtcta gacacaatta gatagtttgt aaagttgcct cgattcgagc
      421 accgtttgcg aaacttgcct acagagttgc gctcggtctg acattccggc ttttatggtt
      481 ggctgtgcca agaatggatc gtcgtcgcat gatgttgatg acggggatag gcgtgatggc
      541 agccgcgatc cccgtccccg aggcccaggc ctacccgtcc ggacagaatg tgccggcgga
      601 ccgggtgcca ccacccgccg cgccgcccgg cggccaagcc gggccctacc ttttccacga
      661 tgagttcgat ggcccggccg gctcagcgcc cgatccctcg aagtggttgg tgtcgcgagc
      721 ccgcgagtca atcaaggacc cgacgtattg ggagctgccc gaaaatgtcg ggcagtatcg
      781 ggacgatcgc cgaaatgtgt tcctcgacgg caagtccaat ctcgtcattc gcgccgcgaa
      841 agacggcagc acctactacg gcggcaaagt ttttagcccg tggcaaggag gcatcggccg
      901 gacctgggaa gcccgaatca aactcaactg cctcactgcc ggttgctggc ccgcctactg
      961 gttgtccagt gacgctcagg gcgaaattga catagtcgag tggtacggca acggcaactg
     1021 ggcttcggga accaccgtcc acgcaagagc gaacggctcc gaatgggcga cccacaacct
     1081 cgcgctggac agcgggtggc acacgtggcg agtccaatgg gatgaggccg gtatgcgctt
     1141 ctggaaggac tacgtcgacg gggcgcagcc atacttcaat gttccggcga actccttggc
     1201 ggattggccg ttcaacgacc ccggctatca ggtgtttccg atcttaaatc tcgcggtggc
     1261 tggctccggt ggcggcgatc ccggtccggg gacctacccc gcggacatgc tcgtcgactg
     1321 gattcgtgtc tggtaagaca gggcggaccg tcgacgagct cgtcgtcacc gactgatcta
     1381 acaagacggt caggtcccac cagctcaggt ctttccccag ccgggttgat tcctcgtcgg
     1441 tgtctgcgat cgactgctcg accgacttcg tgcgcagctc catgtccggt ctcctttggt
     1501 cgatgtcggc tgtttctcta gtactctgct gccccgtgaa taatggtcgc tcggacaagc
     1561 ctcgctccgt ggggcggcga gggaaacacg cggttgtcat cggggccagc atcgcgggcc
     1621 tgtgcgccgg ccgcgtgctg tcggacttct acgacagggt gacgctgttc gaccgcgacg
     1681 atctcccgga gcaccccgcg aaccggtcgg ccgtgcctca gggccggcac gtgcacatcc
     1741 tgatgtcgcg cggcgcgcag gagttcggcg cgctgtttcc cggcctgctc gacgacatgg
     1801 tcgccgcggg cgtgccgatc ctcgaggatc agcccggatc gatccacttc ggcgcggccg
     1861 ggcacgtgct gggcatcccc accgagctgg aggagacgtt caccacctac gtgccgagcc
     1921 ggccccgcct ggagtggcag atccgccgga gcaccgtgtg gctgccgaac gtggagattc
     1981 ggcagcggtc ggtgagcgcg ccgcggttcg acgccgccac gcagcgggtg acgggcgtgg
     2041 tgcttgacga cggggatgtc gtcgaggcag accttgtcgt cgacgcgacc ggccgcggta
     2101 cccggctgcc agtgtggttg tccgagtggg gttttgaccg tccggtcgag gacacggtcg
     2161 acgtcggcat cggttatgcg acgcagcagc tgcggatccc ggacggcctg atcgaggaaa
     2221 aggtggtcgt cgccggcgcg tcgcgcgagc agccggtcgg tatcgggatg ctctattacg
     2281 aggacgccac ctggggcatg accaccttcg gcgtcggcaa ggtggagcca ccgcacacct
     2341 tcgacgaaat gtgcgtggtc gccgacgccg tgctgccgca gcacgcagcg gccatccgcc
     2401 aggccgagcc gatcggcgaa gtggcgttcc acaagtaccc gaccagtcgc tggcgccgct
     2461 acgacaagct gacccgattt cctgccggga tcgtgccgtt cggcgatgcg gtggtcagtt
     2521 tcaatccgac gtacgggcaa ggtatgacga tgacgtcgct gcaggcgggg cacctgcacc
     2581 gggcgctcga atcagccgac gacgacatcg cgcgccagct gaaccgcgcc accgccaaaa
     2641 cgacttaccc ggtgtggacg atgaacgcga tcggcgatgc gatgttccac aacgccaccg
     2701 gcgacatgcc ccggtggtat cggccggtgg gcaacctgtt cgaacagttc ctcggcgccg
     2761 ccgagacgga acccgtcctg gccgaatggt tcttgcgccg gttctcgctg ctcgacagcc
     2821 tgtacatgat cccgtcggcg cggatcgtcg gtcgcacggt gcggcacaac atgcggtgct
     2881 ggctggccga gcgccgccag acacgcgagc gtcaacccga tttcgcggtc accgagcggt
     2941 ccaagtccgg ctccaggtag atgactcgcg ccgcgggcac agcggcacgt attcgagcct
     3001 ccgcgtcgtc gatcaccgcc gcaactgccg gcaggtcgat gtccggcgcc accgcgacct
     3061 tcgcgccgac gagcatctcc tcgggtccga gatattgagt gcgaatatgg atcagccggt
     3121 ccacatgcgc ggtctgctcg atcgcttcgc ggatcgcgcg gtcttgggca ccggtggcgc
     3181 cctccccgat caacaggctg tgcgtctcga ccatcaggat cgccgcgatg gcaccgagca
     3241 gcacgccgat agccaccgtg ccgatgccgt cccagacggc gtcatcggtg acgatcgtca
     3301 atccgacgcc gatgaacgcg aatagcaacc cgatcagtgc gccgaggtcc tcgagcagaa
     3361 cgaccggcaa ctccgggttg cgtgacgtgc ggatgaatcg ccaccagctg cccgaccctt
     3421 tcagcggccg cgactccacg atggccgtgc gtaagctgaa cgattccaac gcaattgcca
     3481 acacgagaat cacgatcgcc accagcggtg acttcagcgg ctccggatgc gagatcttgt
     3541 gatagccctc gtagagcgcg aacaacgaac ccagactgaa cagcaccagc gcgacgacga
     3601 acgagtagaa gtagcggctg cggccgtatc cgaacgggtg cagctgatcg gcttccttgc
     3661 gggcccggcg ttggcccaac agaagcaggg cctggttgga ggtgtcggcc accgagtgca
     3721 ccgactcggc cagcatcgac gagctcctgg tgatcagaaa tccgacgaac ttcgccaccg
     3781 cgatgccggc atttgcggcc aacgccgcaa ggatcgcttt ggtactgccc tcggtcgaca
     3841 cgtcgcgtcc tctcctaaag cccggtgctg acccggaaca actccgccgg cctctccgca
     3901 acgaggcgga tcgggccgtc gtccgcgggc acccacgccg cctgcccccg ctccagggtc
     3961 agcgacgacg acttcgcgtg cagctgcacc gcaccctgag tacacagcag cacctgcggc
     4021 ccatcgtggc gcaccgcggc atcgatctcg tggccgaggt cttccccgtc gatgcgaaga
     4081 cgtgagaccg cgaactcctc ggtcggagcg ccgtagacca attcgagccc ctcgcaacgc
     4141 atttccggcc tcagcgacgc ctcggcgacg gggtggaagt ccaggatccg cagcagctcg
     4201 ggtacgtcga cgtgcttggg cgtcaaaccg ccgcgtaaca cgttgtccga gttggccatc
     4261 acctctaggg ccacgccgcg caggtaggtg tgcaggttgc ccgccggcag gaacattgcc
     4321 tcgcccggct tcatgtgcat cctgttcagc agcagtgccg ccagcacacc ggcgtcaccc
     4381 gggtagcgct cgccgagctc gagcacggcc ttcgcctcgt cagtgaaatc ggctgcgccg
     4441 gaacggacat agtggatcgc cccgtcgagc accgcaggca ccagaacgtc gaggtgcggc
     4501 tgcggcgcgg tgatccaggt ggtgaacagc acccgcaatc cgtcggcgtc gggctgaccc
     4561 ttcagcagct cgacgaacgg gtcgagatcg gccaccgcca gcgcctccat cagttcgatg
     4621 gtgcgcggca cgccgcggaa gccggccagc gcctcgaact gctccagcgc gatcaccaac
     4681 tccggcttgt ggctgcggtc gcggtagttg cgggtgggcg acgtcaccgg gatgccgcgc
     4741 cgctcctcgc ggtcgaaccc
//
LOCUS       DASWBF010000435        10616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_436374,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000435 DASWBF010000000
VERSION     DASWBF010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436374"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..843
                     /gene="ndk"
                     /locus_tag="VF874_16030"
     CDS             433..843
                     /gene="ndk"
                     /locus_tag="VF874_16030"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9177511.1"
                     /translation="MNERTLVLIKPDGVERGLVGEIISRIERKGLSLAALELKTVSDD
                     LARQHYAEHEGKPFFSSLLEFITSGPVVAALVEGPRAVAAFRQIAGGTDPVEKAAPGS
                     IRADYALETQNNLVHGSDSTDSAAREIALWFPGR"
     gene            1152..3569
                     /locus_tag="VF874_16035"
                     /pseudo
     CDS             1152..3569
                     /locus_tag="VF874_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
     gene            3919..4230
                     /gene="rplU"
                     /locus_tag="VF874_16040"
     CDS             3919..4230
                     /gene="rplU"
                     /locus_tag="VF874_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9177512.1"
                     /translation="MATYAIVKTGGKQYKVAAGDVVKVEKLEAEPGATVSLPVALVVD
                     GSDVTTDADKLAEVAVTGEVLEHTKGPKIRIHKFKNKTGYHKRQGHRQQLTVLKVTGI
                     K"
     gene            4242..4499
                     /gene="rpmA"
                     /locus_tag="VF874_16045"
     CDS             4242..4499
                     /gene="rpmA"
                     /locus_tag="VF874_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX9177513.1"
                     /translation="MAHKKGASSSRNGRDSAAQRLGVKRFGGQVVKAGEIIVRQRGTH
                     FHPGVNVGRGGDDTLFAKAAGAVQFGEKRGRKTVSIVPAAE"
     gene            4580..6064
                     /gene="obgE"
                     /locus_tag="VF874_16050"
     CDS             4580..6064
                     /gene="obgE"
                     /locus_tag="VF874_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX9177514.1"
                     /translation="MPRFVDRVVIHVRAGNGGHGCASVHREKFKPLGGPDGGNGGRGG
                     SVYLVVDPQVHTLLDFHFHPHVTAHNGKQGQGGNRDGANGDDLHLKVPDGTVVLDENG
                     RLLADLVGAGTTFEAAEGGRGGLGNAALASRARKAPGFALLGEKGQARDLTLELKTVA
                     DIGLIGFPSAGKSSLVSVLSAAKPKIADYPFTTLTPNLGVVSAGQQSFTMADVPGLIP
                     GASEGRGLGLEFLRHIERCAVLVHVVDCATLEPGRDPVSDIEALEAELAAYSPTLQAD
                     STLVDLSDRPRAVVLNKIDVPEARELADFVHDEVHRRFGWPVFEVSTLTREGLRPLTF
                     ALWQLVSKFRTAHPAAPPRRPVIRPVPVDDSGFTVEPDPERPGAFVVRGSRPERWIGQ
                     TDFDNDEAVGYLGDRLARLGVEDELLRLGATPGCGVTIGDVTFDWEPQTPAGVDVTLS
                     GRGTDVRLERTDRTSAAERKAARRQRRSEADERLREENQTGDTP"
     gene            6061..7170
                     /gene="proB"
                     /locus_tag="VF874_16055"
     CDS             6061..7170
                     /gene="proB"
                     /locus_tag="VF874_16055"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX9177515.1"
                     /translation="MTAREAVRTARSVVVKVGTTALTTSTGMFDVGRLEKLADAIETR
                     MKAGSDVVIVSSGAIAAGLEPLGLTRRPTDLATKQAAASVGQVALVNSWSAAFARYHR
                     TVGQVLLTAHDIAQRVQHTNAQRTLDRLRALRAVAIVNENDTVATNEIRFGDNDRLSA
                     LVAHLVGADALVLLSDVDGLYDSDPRKGNARFIAEVSGPQDLDGVVAGRGHHLGTGGM
                     ASKLSSALLASDAGVPVLLAAASDAATALTDASVGTVFAARRSRMSARRFWVRYAAEA
                     AGALTLDAGAVRAVIEQRRSLLPAGITALSGRFFGGDVVELHGPDAAVVARGVVAYDA
                     TELATMIGRSTSELAPELRRPAVHADDLVALQPRQ"
     gene            7247..8236
                     /locus_tag="VF874_16060"
     CDS             7247..8236
                     /locus_tag="VF874_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HEX9177516.1"
                     /translation="MSAHFPDAETTRAVLALATRAPSVHNSQPWRWRVGARSLHLYAD
                     ASMHLRNIDPDSRDLILSCGASLHHCVVALAAMGWQAKVHRLPNPAEPNHLAAIEVSR
                     QAASELDVTLAAAIPRRRTDRRHYSSWQVPAADIGLMGARAARAGVMLRQVESLPKLQ
                     QLVAEAVLTHATDHDYLTELTAWSGRYGSLAGVPARNTPPSDSTAVIPGRLFAGPVLA
                     QPADASPAEDNAVVLALGTKDDSELSRLRAGEATSLVLLTATALGLASCPVTEPLEVT
                     DTRAAVQEDVFGDSGYAQMLLRVGWAPVNAEPLPSTPRRPLEDVVELLDHQPH"
     gene            complement(8230..8958)
                     /locus_tag="VF874_16065"
     CDS             complement(8230..8958)
                     /locus_tag="VF874_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9177517.1"
                     /translation="MATKPPVVVDTRYHDGVIFDTDGVVTDTASIHGKAWAMMFDDFL
                     STLPARNGEDHSRFTEEDYMRFVDGKPRYDGVADFLASRRISVGAETVRELGDRKQEL
                     FLRSLADGVPRFESTIALVQRLQSAGLATGLYSASRNCERVLKGAGIEDLFPVRVDGV
                     VAAELGLPGKPDPAVLLETARRLQIRPDRCVVVEDAEAGVAAGRNGGFGYVIGVDRTG
                     HRDELLESGADVVVTDLAEVTVRQ"
     gene            9008..9907
                     /locus_tag="VF874_16070"
     CDS             9008..9907
                     /locus_tag="VF874_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9177518.1"
                     /translation="MSAGVSNRGIVVGVDGSDAAKVAVYWAAQEAALRNVPLTLVYVI
                     NPILATWQPAPAPAPVPVEFEEWQQEQARIYMDDAIRTAEENTGNGRPLTINTEVLYA
                     STVPALVNLSNEADMVVVGSRGHGAVARVVLGSVSTGLVHHAHCPVAVIHDEDPLMPH
                     PAQAPVLVGIDGSEASELATAIAFDEASRRGVELVALHAWSDSEVIELPGLDWSAMKT
                     QEEQVLAERLAGWQERYPDVRVNRLVVCDQPARQLIEHSESAQLVVVGSRGRGGFAGM
                     SLGSVSSAVVHAVRMPVIVARGS"
     gene            complement(9912..>10616)
                     /locus_tag="VF874_16075"
     CDS             complement(9912..>10616)
                     /locus_tag="VF874_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9177519.1"
                     /translation="RHKGEQHFDGEQLDMLAGFADQAALAWQLATTQRHLRELDILSD
                     RDRIARDLHDHVIQRLFAVGLSLQGTIPRARSAEVQQRLSNCVDDLQAVIQEIRTAIF
                     DLHGASSGITRLRQRLDEAVSQFSTPEVRTSVQFVGPLSVVSAALADHAEAVVREAVS
                     NAVRHGEATTLRVCVTVEDDLHIEVADNGKGISGDVTGSGLKNLARRARDVGGSFALE
                     PNPGGGTLLRWTAPLE"
BASE COUNT         1792 a         3514 c         3731 g         1579 t
ORIGIN      
        1 taccaggcca gcagcctccg cctgagtagc cgggtaagtc acagccgcct gaggagtcac
       61 cccggctggg ggagtgcttt gtgcccgcct cggagtagcc ctgtgccctg ttgcgaaatg
      121 gtgaaattgt cgctgcaagg cgggcagttc gactattgct cctccgtcgc cctgagatcg
      181 ccggtgtgcc gtcttcgcaa atttcgcgag ttctgcacca gggctgtggt tttcgccgag
      241 tgacgacctt ggtgcagatt tcggcggagt ccagcgacgc gcggacgcga ccgtacggat
      301 accgggtcgc tgcggggcgg gcagttcgaa ccatcaccga cagcagggac tgctgaggcc
      361 atcgtgattg ctgaggtcct gagaagagac ctcgggcacc gggcccgcgc ccggggccgt
      421 agtctgtgcg ccgtgaatga gcggaccctc gttttgatca agcccgacgg cgtcgaacgc
      481 ggacttgtgg gggagatcat cagccgaatc gagcgcaagg gcctgtcgct ggccgcgttg
      541 gaactgaaga ccgtcagcga cgacctcgcg cggcagcact acgccgaaca cgagggcaag
      601 ccgttctttt cgtcgctgct cgagttcatc acctcgggac ctgtggtggc cgcgctcgtc
      661 gaaggcccgc gggcggttgc ggcgttccgt cagatcgccg gtggcaccga cccggtcgag
      721 aaggcggcgc cgggcagcat ccgcgccgac tacgcgctgg aaacgcagaa caacctggtg
      781 cacggatcgg actccaccga ctcggccgcc cgcgagatcg cgctctggtt cccgggacgg
      841 tagcggttcg ccgcgcacta gcggcggtat gggatacttg acgcgggtag tcggcgctac
      901 acggcgctgg atacccggat gaagacttcg acgagcgcga cggtcgcgac atccgcgatg
      961 cggcgcggac cgtcacagcc gtcattcgca ccaggtgccg agagctcccg agccactcgg
     1021 cgcgaagacc tacaacactc gggacaagcg ccccgagttc acagcgatag ccctcgcgtg
     1081 gccgcgtctg ccgacgcgcc cgggggcttc taggagatta cgtggctgac gatgacctct
     1141 cacaagatca acccgacagc agcgctcagc ctgatcagaa cccgcaagac cagctgagcg
     1201 tcgaaacgct gacgccggag ccggccgacg gactccccgg cgaagctgat tacatgccgc
     1261 tgttcgtcgc cccccagccg gtcgggcgcg ccgacgtcga cgacgaggac gacgaggacg
     1321 acgacgaatc cgacgagggc gagcaggccg aacggcccgc gaaccggcgc cggcgtcggg
     1381 gccgccgtgg ccgtggtcgc ggccgcggcg agcagggctc agccgacgga gacgactccg
     1441 acaccgacga ggcctcggag agcgacgaat ccgacgagga cgacgagacc ggcgatgagg
     1501 acaccgccac gacggaggcg gccacgcgca ggcgccgccg ccgtcgtcgc cgcaaaaccg
     1561 gcgcgggcga cgacgcggac gcgtcgtccg acgacccgcc caacacggtc gtgcacgaac
     1621 gcactccgcg gtcaaatggc aaggacgaca acgagattca gggcatcact ggatccaccc
     1681 ggttagaggc caagcggcag cgccgccgcg acggacgcga cgccggccgg cgccgtccgc
     1741 cgattctgtc cgaggccgag tttctcgcgc gtcgcgaggc cgtcgagcgc gtgatgatcg
     1801 tgcgggacaa agtgcgcacc gagccgccgc acgagggcgc ccgctacacc cagatcgcgg
     1861 tgctcgagga cggcgtgctc gtcgagcact tcgtcaccag cccggcatcc gcctcactgg
     1921 tcggcaacat ctatctgggc atcgtgcaga acgtgttgcc gtcgatggag gccgcgttcg
     1981 tcgacatcgg ccgcgggcgc aacggcgtgt tgtatgccgg cgaagtcaat tgggacgctg
     2041 cgggactcgg cggtacgaac cgcaagatcg agcaggcgct caagccgggg gactacgtcg
     2101 tcgtccaggt cagtaaggac cccgtgggcc accagggagc gcggctcacc acgcaggtgt
     2161 cgctggccgg gcggtatctg gtgtatgtgc cgggcgcgtc gtcgaccggg atcagccgca
     2221 agctgcccga caccgaacgc cagcggctca aggagattct gcgcgaggtc gtgccgtccg
     2281 atgccggcgt gatcatcagg accgcctcgg agggcgtgaa ggaagacgac atccgcgccg
     2341 acgtgaaccg gctgcaggag cgctggacgc agatcgaggc gcaggcccag gagatcaaag
     2401 gcaaggccgc cggggctgcc gtgccgctgt acgaggaacc cgacgtgctg gtcaaggtca
     2461 tccgcgacct gttcaacgag gatttctcgg gtctcatcgt atccggcgac gaagcgtgga
     2521 acacgatcaa cgactacgtg aattccgttg cgccagacct ggttccgcgg ctcaccaaat
     2581 acgagcccgc ggcaccggag ggtcctgacg tgttcgcggt gcaccgcatc gacgagcagc
     2641 tggccaaggc gatggaccgc aaggtgtggc tgccgtccgg cggcacgctg gtgatcgacc
     2701 ggaccgaggc gatgaccgtc gtcgacgtca acaccggcaa gttcaccggc tccggcggca
     2761 acctcgagga aaccgtcacc cgcaacaacc tggaggccgc cgaggagatc gtgcggcagc
     2821 tgaggctgcg ggacatcggc ggcatcgtgg tgatcgactt catcgatatg gtgctggagt
     2881 ccaaccgcga cctggtgttg cgcaggctca ccgaggcgct ggcccgggac cgtactcgcc
     2941 accaggtgtc ggaggtcacg tcgctgggcc tggtgcagtt gacgcgcaag cgcctcggga
     3001 ccgggctggt cgaagcgttc tcgacgccct gcccgcactg cggcggccgg gggattctgg
     3061 tgcacagcga tcccgtcgaa tcggccgcgg ccagccgcaa ggagtccggc gggcggcgcg
     3121 gcaagcgctc gcgcaagtcc cggggggacg agccggcgat cgcgaaaatc cccgcccatg
     3181 cccccggtga gcatccgttg ttcaaggcga tggccgccgc gcgcagtgag gaggacgagg
     3241 aggtcttcga cgaggaggtc ttcgaggagg actcagccga gacggccgag tcggctgatg
     3301 agctgaagga ggccatcgtc gatctggccg aggaagacct cgaggaggac ctcgacgaat
     3361 cggacgagga cgacgaagaa gacgaagaag acgaggacga ggagatcgac gtcgacctcg
     3421 acgaggacga cgaagacctc gacgacgatc tggacatcga cctcgacgag gaagacgagc
     3481 ccgtcgtgac cgcgccggct gccgtgcagg cccgtcggcc ccggcgctcc gccgcgcgtc
     3541 cggccgggcc acccagccac gaggactgac cgcggcgagc gctcgggggg agggcgggtg
     3601 tcgctccggg gcgggcagaa gcatctccat tgcctggaga ggcaagtggg acaactgagg
     3661 ctcatgtggc ctcggcgatc acttcagcca cacatgtcac tgaggagggt aagactcgcc
     3721 gtgcccggct atcagcgaca agcgtgcggt cggcgcggag tcaagatgca cgctccgctc
     3781 ggcgacgaaa gctagctggt ttgacccctt cggcgctggt cacgtaccct tgaccagttg
     3841 tcgccaggcc ggtgtcggcg gcaaaacccc agacccgcac gtgccccacg gtgtggcgtg
     3901 tgcccagaaa gaggagcgat ggcgacctac gcaatcgtca agaccggcgg caagcagtac
     3961 aaggtggccg ccggcgacgt ggtcaaggtg gagaagctcg aggctgagcc cggtgcgacg
     4021 gtgtcgctgc ccgtggcgct ggtcgtcgac ggctccgacg tcaccaccga cgcggacaag
     4081 ctcgccgagg tcgcggtgac cggcgaggtg ctcgagcaca cgaagggccc caagatccgc
     4141 atccacaagt tcaagaacaa gaccggctac cacaagcggc agggtcatcg tcagcagctg
     4201 accgtgctga aggtcaccgg gatcaagtag gggagcgaca gatggcacac aagaagggcg
     4261 catccagctc ccgcaacggc cgcgactccg ccgcccagcg gctaggcgtc aagcggttcg
     4321 gcggccaggt cgtcaaggcc ggcgagatca tcgtccggca gcgcggcacc cacttccacc
     4381 cgggcgtcaa cgtcgggcgc ggcggcgacg acacgctgtt cgcgaaggcg gccggcgcgg
     4441 tgcagttcgg cgagaaacgc ggccgcaaga cggtcagcat cgtccccgcc gccgagtgag
     4501 ccgcgccgag agcgacgttt tgcaggccgc cactcgtact tttcctgcca aacgtcgctt
     4561 tcggtgaaag gaatcgccga tgcccaggtt tgtcgaccgc gtcgtgattc acgtgcgggc
     4621 cggcaatggc ggacacggct gcgcgtcggt acaccgggag aagttcaagc cactcggcgg
     4681 cccggacggc ggtaacggcg gccgcggcgg cagcgtgtac ctggttgtcg atccgcaggt
     4741 gcacacactg ctggatttcc atttccatcc gcacgtcact gcgcacaacg gcaagcaggg
     4801 tcagggcggc aaccgcgacg gcgccaacgg cgacgatctg cacctgaagg ttcccgacgg
     4861 caccgtggtg ctcgacgaga acggccgcct gctggccgat ctggtcggcg cgggcaccac
     4921 attcgaggcc gctgagggcg gccgcggcgg cctgggcaac gcggcgctgg cgtcccgcgc
     4981 acgcaaggcg ccgggcttcg cgctgctcgg cgaaaaaggc caggcccgtg acctgaccct
     5041 cgagctcaag acggtcgccg acatcgggct gatcggattt ccgtcggcgg gcaagtcgtc
     5101 gctggtgtcg gtgctctcgg cggcaaagcc caagatcgcc gactatccgt tcacgacgtt
     5161 gacgcccaac ctcggcgtcg tgtcggcggg gcagcagtcg ttcacgatgg ccgacgtgcc
     5221 cgggctgatc cccggcgcgt cggagggccg cgggctgggg ctcgaatttc tgcggcacat
     5281 cgagcggtgc gcggtgctgg tgcatgtggt cgactgcgcg acgctggaac ccggccgcga
     5341 cccggtctcc gacatcgagg ctctcgaggc ggagctagcg gcgtattcgc cgacgctgca
     5401 ggcggattcg acgctcgtcg acctttccga ccggccgcgg gcggtggtgc tgaacaagat
     5461 cgacgtgccg gaggcgcggg agctggcgga tttcgtccac gacgaggtgc atcggcgctt
     5521 cggctggccg gtgttcgagg tgtcgacgct cacccgggaa gggctgcggc cgttgacgtt
     5581 tgcgctgtgg caactggtgt ccaagttccg tactgcacat cctgcggcgc caccgcgccg
     5641 gccggtgatc cgcccggtac ccgtcgacga cagcggtttc accgtcgaac cggatcccga
     5701 gcgtccgggc gcgtttgtgg tgcggggaag caggcccgaa agatggatcg gacaaaccga
     5761 tttcgacaac gacgaagccg tcggttatct cggcgaccgg ctggcgcggc tcggcgtgga
     5821 ggacgagctg ctgcggctgg gcgccacccc gggctgcggg gtgacgatcg gcgacgtgac
     5881 gttcgactgg gagccgcaga ccccggccgg ggtcgacgtg acgctgtcgg gccgcggcac
     5941 cgacgtccgg ctcgaaagaa ccgaccgcac ctcggccgcc gaacgcaagg cggcccggcg
     6001 tcaacggcgc agcgaggccg atgagcgctt gcgcgaagag aatcagaccg gagacacacc
     6061 gtgacggccc gggaggcggt acgcacagcg cgcagcgtgg tggtgaaagt cggcaccacc
     6121 gcgttgacca cgtccaccgg catgttcgac gtcgggcgac tggagaagct tgccgacgcg
     6181 atcgaaaccc ggatgaaggc cggctctgat gtggtgatcg tgtcctcggg cgccatcgct
     6241 gccgggctcg agccactcgg attgacccgc cgcccaacgg atctggcgac caaacaggca
     6301 gccgccagtg tcggccaggt ggcgctggtc aactcgtgga gcgcggcgtt tgcccgctat
     6361 caccgcaccg tgggccaagt gctgctcacc gcgcacgaca tcgcacagcg ggtgcagcac
     6421 acgaacgccc aacgcacatt ggaccggctg cgcgccctgc gcgcggtggc gatcgtcaac
     6481 gagaacgaca ccgtggccac caacgagatc cggttcggcg acaacgaccg gctctccgcg
     6541 ctcgtcgcgc atctggtcgg cgccgacgcg ctggtgctgc tgtcggacgt cgacggcctg
     6601 tacgactcgg acccacggaa aggcaacgca cggttcatcg ccgaagtcag cggaccacaa
     6661 gacctcgacg gagtggtcgc cggccgcggc caccacctgg gcaccggcgg gatggcctcc
     6721 aagctgtcgt cggcgctgtt ggcgtcggac gccggtgtcc cggtgctgct ggccgcggcc
     6781 tccgacgcgg cgacggcgct caccgacgcg tcggtgggca cggtgttcgc cgcgcgtcgg
     6841 tctcggatgt cggcccgccg gttctgggtt cggtacgccg ccgaggccgc cggtgcgctg
     6901 acgttggatg ccggggcggt gcgcgccgtg atcgagcagc gccggtcact gctgccggcg
     6961 ggcatcaccg cattgtcagg ccggtttttc ggcggcgacg tggtcgagct gcacggacct
     7021 gacgcggctg tggtggcgcg cggcgtcgtg gcctacgacg cgacggaact ggccacgatg
     7081 atcgggcgct cgacgtcaga actggcgcct gagctgcgcc gcccggctgt gcacgccgac
     7141 gacctggtcg cccttcagcc ccgtcaataa aggtcttttt tccctacccg ccggcgatcc
     7201 gtgaaggcag cctgagtgca gggtcgttcc agaaagcagg gagttcatga gcgctcattt
     7261 ccccgatgcc gagacgacac gtgcggtgtt ggcgctcgcc acccgcgcac cgtcggttca
     7321 caactcgcag ccgtggcgtt ggcgggtcgg cgcccggagt ctgcacctgt acgcagacgc
     7381 gtcgatgcac ctgcgcaaca tcgatccgga cagccgtgac ctgattctgt cctgcggtgc
     7441 ctcactgcac cactgtgtcg tcgcgctggc ggcgatgggg tggcaggcga aagtgcaccg
     7501 actgccgaat ccggcagagc cgaatcacct tgccgccatt gaggtgtccc gccaagcggc
     7561 cagcgagctg gatgtcacgc tggctgccgc gattccgcgg cggcggaccg accggcggca
     7621 ctacagctcc tggcaggtgc ctgccgctga catcggcctg atgggtgcgc gtgcggcccg
     7681 cgccggagtg atgctgcgtc aggtggagtc gctgccgaag ctgcagcagc tcgtggccga
     7741 agcggtgttg acgcacgcca ccgaccacga ctatctgacc gaattgactg cgtggagtgg
     7801 gcggtacggg tcgttggccg gtgtgccggc gcgcaacacc ccgccatcgg actcgacggc
     7861 ggtcatcccg ggtcggctgt tcgcgggccc cgttctggct cagccggccg acgcctcccc
     7921 ggccgaggac aacgccgtcg ttcttgcgct gggcacgaaa gacgacagcg agctgtcgcg
     7981 cctgcgcgcg ggcgaggcga cgagcctcgt gctgctcacc gcgaccgcgc tggggttggc
     8041 aagctgcccg gtgaccgagc cgctcgaggt caccgacacg cgtgccgcgg tgcaggagga
     8101 cgtcttcggc gacagcgggt acgcccagat gctgctgcgg gtggggtggg caccggtcaa
     8161 tgccgaaccg ctaccctcga cgccgcgccg cccgctggag gacgtcgtcg agctgctcga
     8221 ccaccagcct cactgacgga cggtaacctc ggccaggtcg gtcaccacga cgtcggctcc
     8281 ggactccagc agttcatcac ggtgaccggt ccggtcgact ccgatgacgt agccgaaccc
     8341 tccgttgcgc cccgcggcca ccccggcctc ggcatcctcg accaccacgc accgatcggg
     8401 acggatctgc aacctgcgcg cggtctccaa caacaccgcg gggtcgggct tgccgggcag
     8461 ccccagttcg gcggcgacga caccgtcgac acggaccggg aagagatcct cgatgccggc
     8521 ccctttcagc acgcgttcgc agttgcggct cgccgagtac agcccggtgg ccagcccggc
     8581 gctctgcagc cgctgcacca gcgcaatcgt cgactcgaac ctgggcaccc cgtcggcgag
     8641 cgaccgcaag aacagctcct gcttgcggtc gcccaactcg cggaccgttt cggccccgac
     8701 cgaaatgcgg cgtgacgcca agaaatccgc caccccgtcg tagcggggct tgccgtcgac
     8761 gaaacgcatg taatcctctt cggtgaaccg agagtggtcc tccccgttgc gcgccggaag
     8821 cgttgacagg aagtcgtcga acatcatcgc ccacgccttg ccatgaatgg acgccgtgtc
     8881 ggtgaccacg ccgtcggtgt cgaagatgac gccgtcgtga taccgggtgt cgacaacgac
     8941 cggtggcttc gtcgccatga cacgatgcta gacaggtcct tggaaggctc gctcgaagga
     9001 ggccggaatg tcagctgggg tcagcaaccg cggcatcgtc gtcggtgtgg acggatccga
     9061 cgccgccaag gtcgccgtct actgggccgc gcaggaggcg gcactgcgca atgtgccgct
     9121 gacgctggtg tatgtgatca acccgattct cgccacctgg cagccggcgc cggcgccggc
     9181 gccggtgccc gtcgaattcg aagagtggca acaagagcag gcccgcatct acatggacga
     9241 tgcgatccgc acagccgagg agaacaccgg caacgggcgt ccgctcacga tcaacaccga
     9301 ggtgttgtac gcctccactg tgcccgcgct ggtgaacctt tccaacgagg cggacatggt
     9361 cgtggtcgga agccgcggac acggcgcggt cgcccgggtc gttctcggat cggtgagcac
     9421 cgggcttgtc catcacgcgc actgcccggt cgcggtcatc catgacgagg acccgttgat
     9481 gccgcatccc gctcaagcac cggtgctggt cggcattgac ggctccgagg cgtcagagtt
     9541 ggcgacggcg atcgcgttcg atgaagcgtc ccgccggggc gtcgagctgg tggccctgca
     9601 tgcctggagt gactcggagg tcatcgaact gcccggtctg gactggtcgg cgatgaaaac
     9661 ccaggaggaa caggtactcg cggagcggtt ggccgggtgg caggagcgct atccggatgt
     9721 cagggtgaat cgcctcgtcg tctgcgacca gccggcgcgc cagctgatcg agcactccga
     9781 gtcggcccag ctcgtcgtgg tcggcagccg cggccgcgga gggttcgcgg gaatgtcact
     9841 gggatcggtc agctccgcgg tggtgcacgc cgtccggatg ccggtgatcg tcgcccgcgg
     9901 gtcgtaacga cctattcgag cggcgcggtc cagcgcagca gcgtgccgcc gccggggttg
     9961 ggctccagcg cgaagctgcc gccgacgtcg cgtgcgcggc gggcgagatt tttcagtccg
    10021 ctgccggtga cgtcgcctga gatgccttta ccgttgtcgg cgacctcgat gtgcaagtcg
    10081 tcctcgacgg tgacgcaaac ccgcaaagtg gtcgcctcgc cgtgccgaac cgcgttcgaa
    10141 accgcttcgc gcacaacggc ttccgcgtgg tccgccagag ccgcgctgac gacggacagc
    10201 gggcccacaa actgcaccga ggtgcggacc tcgggcgtgg agaactgcga gacggcctcg
    10261 tccagccgtt gccgcagcct cgtgataccc gacgaggcgc cgtgcaggtc gaagatcgcg
    10321 gttctgatct cctggatcac cgcttgcagg tcgtcgacgc agttcgaaag tcgttgctgc
    10381 acttcggctg accgagcgcg ggggatcgtg ccctgcagcg acaacccgac ggcgaacagg
    10441 cgctggatca cgtggtcgtg caggtctcgt gcgatccggt cgcggtcgct gaggatgtcc
    10501 aactcacgta gatgacgctg ggtggtggcc aactgccatg ccagtgcagc ctggtcggcg
    10561 aagccggcga gcatatcgag ctgttcgccg tcgaaatgct gctcgccctt gtgtcg
//
LOCUS       DASWBF010000436         4889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_52419, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000436 DASWBF010000000
VERSION     DASWBF010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52419"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VF874_16080"
     CDS             complement(<1..575)
                     /locus_tag="VF874_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9177520.1"
                     /translation="MTAEAPPLGGLEARRPFPARLGPKGNLIYRVITTTDHKLIGIMY
                     MVACMVFFFIGGLMALLIRAELAVPGLQFLSNEQYNQLFTMHGTAMLLFYATPIVFGF
                     ANIVLPLQIGAPDVAFPRLNALSFWLFVFGALIALSGFITPGGAADFGWTAYSPLTDA
                     IHSPGAGGDLWILGLGVGGLGTILGGVNMITT"
     gene            704..1768
                     /locus_tag="VF874_16085"
     CDS             704..1768
                     /locus_tag="VF874_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9177521.1"
                     /translation="MLKAGLVVAGVAVLTLVTGCGGKPTGGTAPGQPSVITSTTKIAD
                     AGVLGNQRRPDESCAPQPAAPDPGPPVRPVRHAAGETDVPAEPQRIVVLSGDQLDALC
                     ALGLQSRIVAAALPDGSSSQPSYLGTVIHDVRGAGTRSAPDLSAIRAAGPDLILGSQA
                     LTPELYPSLSAIAPTVFTGAPGAAWQDNLRLVGAATARADAANGLVDGFNAFAAKAGA
                     DNDAAHFQASVVQLTDNTARIYGAANFPASVLTDVGVDRPASQRFTDKPYIEIGTSDL
                     GDADFSAADADVVYVSFASQAAKERAQEILQSDAWRRLSANRDNRVFIVNNEVWQTGE
                     GIVAARGIVADLKFLNAPIN"
     gene            complement(1765..3666)
                     /locus_tag="VF874_16090"
     CDS             complement(1765..3666)
                     /locus_tag="VF874_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177522.1"
                     /translation="MSAPPRVNRLVMYKHGVAFVERGGPVDGDFDLLFRRDDMKDVLK
                     SLSVDVADGQLSVRAVAFDIPSDPREELEDRNLLFESGQALLGLLDGLRGCAVDVHCG
                     DQRHRGEVIGVDDGAGNGQAHRLLVLRAEAGTVNLVDLADAHRIEVLEEPSRGDLDYL
                     IDRSRAATAGENRSVSIRVGGRADDARVSYIVPAPVWRVSYRVIHDGDAMVLVAMGIV
                     HNPLDEDLTEVELTLTTGQPISFDIDLYHGKHVQRTVVEEAERVYTPPAAKARRHYAA
                     AELSSAYAMSGGPYEEAAEDVETGDRGEHFEYRLTNPISLKRGGAAMVPLAVAPVDQA
                     RRELVWRDGDEPAPDIVLAFTNTTDVVLEEGPAVVYEEGSYAGEAMVPFTSRGANVRL
                     SFAKDLAVRCRRTSTLDILTTRIRLAADAIVAEQRQEERHTLRAENDHDETVDVVFEL
                     SKTPGHAVVPIDCVIEAGDDGDWHRFRVSVPGHAVVEATALETWPVYREIDYEQLSPG
                     QLEEWLAGHSLDAATVGALSGVLAAWERATRMDTRREELEAERADVYATQSRIAEQLN
                     VLASDGPEGDLRRRHVRELELTQDRVDALDAQIRGLRDDANDARRAASAELQRVIDTS
                     SAEDDVPRP"
     gene            complement(3743..4069)
                     /locus_tag="VF874_16095"
     CDS             complement(3743..4069)
                     /locus_tag="VF874_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HEX9177523.1"
                     /translation="MTATSLFQSILQWLRAGYPEGVPGPDRVPLLSLLRNTPLTDDQI
                     KEVVREITEEEAKARPGHEIDHDEIEKFIEGVAHHDGGPDNIRRVAAKLAAAGWPLAG
                     TGNSAD"
     gene            4381..4575
                     /locus_tag="VF874_16100"
                     /pseudo
     CDS             4381..4575
                     /locus_tag="VF874_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          836 a         1657 c         1587 g          809 t
ORIGIN      
        1 gtcgtgatca tgttgacgcc gcccaggatg gtgcccaaac cacccacacc caatcccaga
       61 atccacagat ccccgcccgc acccgggctg tggatggcat cggtcagcgg gctgtaggcc
      121 gtccagccga agtccgccgc ccccccggga gtgatgaacc cgctgagcgc gatcagcgcc
      181 ccgaacacaa acagccaaaa cgacagcgcg ttcagccgcg ggaacgccac atccggggcg
      241 ccgatctgca gcggcaacac gatattggcg aaaccgaaca cgatcggagt cgcataaaac
      301 agcagcatcg ccgtgccgtg catggtgaac aactggttgt actgctcgtt ggacaagaac
      361 tgcaacccgg gcaccgccaa ctcagcgcgg atcagcagcg ccatcagccc gccgatgaaa
      421 aagaacacca tgcacgcgac catgtacatg atcccgatca gcttgtgatc ggtcgtagtg
      481 atcacccggt agatcagatt gcccttagga cccaaccgag ccgggaacgg gcgacgagcc
      541 tcaagtcctc cgagaggggg cgcttccgcg gtcaagagct cctccaaaag ttcgcgcgca
      601 ttgcggaact aaatcctaga ccgcgacggt tcgcccggtc ggggccggtc ctacaaactg
      661 tcgtacacct ttgcccagac cggtgggtgt taccgtcggc gacgtgctca aggcgggtct
      721 ggttgttgcc ggggtcgcgg ttctgacgtt ggtgactggc tgcggcggca agccaaccgg
      781 tggcacagcc cccggtcagc cgtcggtgat cacgagcacc acgaagatcg ccgacgcggg
      841 cgtgctgggc aaccagcgca ggccagacga atcgtgcgcg ccgcagcccg cggcgcccga
      901 ccccggtcca ccggttcggc cggttcgcca cgccgccgga gagaccgacg tgccggccga
      961 accacagcgc atcgtcgtgc tgtccggtga ccaactcgac gcgctgtgtg cgctgggctt
     1021 gcagtcgcga atcgtcgccg ccgcgttgcc cgacggctcg tcgagtcagc cgtcgtattt
     1081 gggcacggtc atccacgacg tgcgcggcgc cggcacccgc agtgcccccg acctgtccgc
     1141 gatccgtgcc gccggccccg atctgatcct cggatcgcag gccttgacgc cggagctgta
     1201 cccgtcactg tccgcgatcg ccccgacggt gttcaccggc gcgcccggcg ctgcgtggca
     1261 ggacaaccta cggctcgtcg gggcggcgac ggcgcgcgcc gatgcggcca acgggctggt
     1321 cgacggcttc aatgcgttcg cggcgaaggc gggcgccgac aacgacgcgg cgcacttcca
     1381 ggcgtcggtg gtgcaactga ccgacaacac cgcgcggatc tacggggcgg ccaacttccc
     1441 ggcgagcgtg ctgaccgacg tcggcgtcga ccggcccgcg tcgcagcggt tcaccgacaa
     1501 gccgtacatc gagatcggca cttctgatct cggcgacgcg gacttctccg ccgccgacgc
     1561 cgacgtcgtg tacgtgtcgt tcgcctcgca ggcggccaag gagcgggcgc aggagatcct
     1621 gcagagcgat gcgtggcgcc gattgtcggc gaaccgcgac aaccgggtgt tcatcgtcaa
     1681 caacgaggtg tggcagacgg gcgagggcat cgtcgccgcg cgcggcatcg tcgcggactt
     1741 gaaattcctc aacgcgccga tcaattaagg tcgcggcacg tcgtcctcgg cgctggacgt
     1801 atcgattacc cgctgcaatt cggcagatgc ggcccgacgg gcatcgttgg catcgtcgcg
     1861 cagcccacgg atctgcgcgt cgagcgcatc gacccgatcc tgcgtgagtt ccaactcacg
     1921 gacatgtcga cgtcgcaagt cgccctcggg accatccgag gcgagcacat tgagctgttc
     1981 ggcaattcgg ctttgcgtgg catagacgtc ggctcgttcg gcttccagct cctcgcgtcg
     2041 ggtgtccatc cgtgtcgcgc gttcccacgc ggcgagaaca ccggacagcg caccgacggt
     2101 ggccgcatcc agcgaatgac cggcgagcca ctcctcgagt tgacccggtg acagctgctc
     2161 gtagtcgatc tcccgataaa caggccatgt ttcgagcgcc gtagcctcga ccaccgcgtg
     2221 cccaggcaca gacacccgga agcgatgcca gtcaccgtcg tcgccggcct cgatgacgca
     2281 atcgatcggc acgacggcat gaccgggtgt cttcgacagc tcgaagacca cgtcgacggt
     2341 ttcgtcgtgg tcgttttccg cccgcaacgt gtggcgctcc tcttggcgct gctccgcgac
     2401 aatcgcgtcg gcggccaacc gaattcgagt ggtcaggatg tcgagcgtcg aagtgcgccg
     2461 gcagcggacc gcgagatcct tggcgaagga cagccgaacg ttcgcgccgc gcgacgtgaa
     2521 cggcaccatc gcttctcccg cgtagctgcc ctcctcgtac accacagcgg gcccttcttc
     2581 gagcacgacg tctgtcgtgt tcgtgaacgc cagcacaatg tcgggggcgg gctcgtcgcc
     2641 gtctcgccac acgagctcgc ggcgcgcttg atcgactggg gcgacagcca gcggaaccat
     2701 ggccgcgccg ccgcgtttca gcgagatggg gttggtcagc cggtattcga aatgctctcc
     2761 gcggtcgcct gtttcgacgt cctcggcagc ctcctcgtac ggtccgccac tcattgcgta
     2821 ggccgaagag agctccgccg cggcatagtg tcgcctcgcc ttcgcggctg gcggcgtgta
     2881 gacgcgttca gcctcctcga cgacggttcg ttgcacatgc ttgccgtggt agagatcgat
     2941 gtcgaacgag atcggttgcc ctgtggtgag agtcaattcg acttcggtga ggtcctcgtc
     3001 gagcggattg tgaacgatgc ccatcgccac cagcaccatc gcatcgccgt cgtggatcac
     3061 ccggtaggac acgcgccata ccggcgccgg gacgatgtag gacacccgcg cgtcgtcggc
     3121 gcggccgccg acacgtattg acacgctgcg gttctcaccc gccgtcgctg cgcgcgaccg
     3181 gtcgatcaga tagtcgaggt cgccgcgcga gggttcctcc aacacctcga tgcggtgcgc
     3241 gtcagcgagg tcgacaaggt tgacggtgcc ggcttcggca cgcaggacga gcaaccgatg
     3301 cgcctgccca ttcccggcgc cgtcgtcgac gccgatcacc tcgccgcggt gccgctggtc
     3361 gccgcagtgc acgtcgaccg cgcagccacg cagcccgtcg agcaggccga gcaacgcctg
     3421 cccggactcg aacagcaagt tgcggtcctc cagctcctcg cggggatcgg agggaatgtc
     3481 gaacgcgacg gcccgcaccg acagttgacc gtcagctaca tcaacggaca gcgacttcag
     3541 cacgtctttc atgtcgtcac gacggaacaa caggtcgaag tcgccgtcga cagggccgcc
     3601 gcgctcgaca aaggcgacgc cgtgcttgta catgactaac cgatttactc tgggcggtgc
     3661 gctcattcgc ggactataac gccggccccc gacaggtcag ccccggtgaa aaacctcgtc
     3721 gtcggccgtc ggagtcgggc tgctagtccg cggagttgcc ggtgccggcc agtggccagc
     3781 ccgctgccgc gagcttggcc gcgacgcgcc tgatgttgtc agggccgcca tcgtggtggg
     3841 cgacaccctc gatgaacttc tcgatctcgt cgtggtcgat ctcgtgccct ggacgcgcct
     3901 tcgcctcctc ttcggtgatc tctcgcacga cctctttgat ctggtcatcg gtgagcggtg
     3961 tgttgcgaag aagcgatagc agcggcactc gatcaggccc gggaacgccc tcggggtagc
     4021 cggcgcgaag ccactgcagg atcgattgga acagcgacgt agcggtcacg cggctaagtc
     4081 tcgtgcctgg gagacacgga cgccacggtg agattgcaca gtttctcgcg cgttcaccgt
     4141 gtgttcacgc gtggcgtcga tccgttgaag tccgctgcgc atgcgctggt ggcggcatac
     4201 catgtcggtc tatccgccgc cgaaccgccg gtatcggagt cgcggtgtcc gggatacacc
     4261 ccggccgact gccaggccag cgagcagtat cccgaggacc ctttcctcgt acggctcggc
     4321 tgcggcccga acagccagcc cggtcggcgg ctgccctcgg gcacgatcct ggtcgatgtc
     4381 gcggcggcgg gtgacgaacg tgtggtgctg tctgcgcggg tgtccagcca tgatcaatgt
     4441 gccgatctgg atcggcaggt ggctgggttg actgagtggg ccaccgatgc cgggatggag
     4501 gtcggccagt cggtggccga ggttggttgc ggattgaacg gcaagcgtcc aaagctggcc
     4561 cggatcttgt cggacggcac cgaactggtg accgcacctc gatggtttcc ctcgacccag
     4621 ttgtgttcgc gctgtgcagc gaaaaccaag ctcgggctgc gcgaccgcac ctaccgttgc
     4681 cgtaacggat gcccgccttt gttgtcggga cctcaatgcg gcgatcaacc tcgcccgcgt
     4741 cggcgagccc acccgcgggg aaaccggtac cgggagtagc ccggccgcca gcatcacggc
     4801 tggacacgga cgtggagcca accagaagac cagccccacc accacggtgg cggggacggc
     4861 aggaggcgac gaaacgtcaa ccccgcaca
//
LOCUS       DASWBF010000437         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1298338,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000437 DASWBF010000000
VERSION     DASWBF010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298338"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VF874_16105"
     CDS             <1..356
                     /locus_tag="VF874_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mycobacterial-type methylenetetrahydrofolate
                     reductase"
                     /protein_id="HEX9177524.1"
                     /translation="FAKSTDHRPEILLSFGFVPKVENRVGLIDWLTQDPGNAAVADEQ
                     AFIKKLAGSEAAQKRQLLVDLYKRVIDGVADLGFPLSIHLEATYGVSAAAFETFAEML
                     DYWAPSRPEQQAVAR"
     gene            complement(363..1514)
                     /locus_tag="VF874_16110"
     CDS             complement(363..1514)
                     /locus_tag="VF874_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177525.1"
                     /translation="MTDAGISDAFVERLAQRAQEAEELRRLPAATVAELKESGFIDLL
                     KPARYGGQQAEFRAILDPVRRMAHGCASTAWTAAFYTLHNWMLALFDEQAQAEAFATR
                     PFLAPAPLAPTGRAVPVDGGVRLNGRWSWATGVMDGNWAIVGALCGPDDAIYPVLALL
                     PADDIRIVDVWHTDGMRATGSNDVVVEDVFVPTHRLVKVVDIYRGSTPGAHLHDAALY
                     RWPMVPALALVAAMPAFGAAERVAEIYTERLSERVLAYEGTRQKDKPAAQARLAEARV
                     RLRALHGLLADAAERIEEILAAGAHVPRPVRADARLAAAHIVRESRAVIGVLLEASGA
                     SAHFLSCPLQRAKRDVDVISGHVVFDYDVSRELAGALAIGLKVSPIAMV"
     gene            complement(1525..>2296)
                     /locus_tag="VF874_16115"
     CDS             complement(1525..>2296)
                     /locus_tag="VF874_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HEX9177526.1"
                     /translation="GIVRSAALDDAETEQSRFTVRAGTDPTGSLNASADRLRIDLDDV
                     HLDLRFDDPFMWPKAFAGGGIFSSIPFLNQYWHPYRLGGKSSGTVEFSEASWSFNEAR
                     WYSERNWGAGFPERWWWGQAHDFGGDDVSVAFSGGLLKLGPLGRDVAGVVVRLGDRVI
                     RVTPPALVHSHVSDARWTVRARGLRYQIDLDGDGTHLEPHVLPVPLAAERRNVDTDFE
                     HLAGRLRCTVREFGRVVFEGTSELAGLEVGSLPGAPPP"
BASE COUNT          386 a          788 c          771 g          351 t
ORIGIN      
        1 agttcgccaa gagcacggat catcgcccgg agatcctgtt gtcgttcggc ttcgtgccga
       61 aggtcgagaa tcgggtgggt ctgatcgact ggctcaccca agacccaggc aacgcggccg
      121 tggccgacga gcaggcgttc atcaagaagc tggcgggcag cgaagccgcg cagaaacgcc
      181 agcttctcgt cgatctgtac aagcgggtga tcgacggcgt cgccgacctt ggctttccgc
      241 tgagcatcca cctcgaggcg acgtacggcg tttccgcggc ggcgttcgaa acgttcgcgg
      301 agatgctcga ctactgggcc ccgtcaaggc ctgaacagca ggccgtcgct cgttagctcc
      361 cgctacacca tcgctatcgg cgacaccttc aggccgatcg ccaacgcacc ggccaactcc
      421 cggctgacgt cgtagtcgaa cacgacgtgc ccgctgatca cgtcgacgtc acgtttggca
      481 cgttgcagcg ggcagctcag aaaatgtgcg ctggcgcccg atgcttctag cagcaccccg
      541 atcacggcgc gcgattcccg cacgatgtgc gcggctgcca gccgggcgtc ggcgcggacg
      601 ggacgcggga cgtgggctcc ggccgcaagg atctcctcga tccgctcggc ggcatcggca
      661 agcagcccgt gcaacgcgcg cagccgaaca cgtgcttcgg ccagtcgggc ttgagcggcc
      721 ggcttgtctt tctgccgggt gccctcgtag gcgagcacac gctcggagag cctttcggtg
      781 tagatctcgg cgacgcgctc ggccgcgccg aacgcgggca tcgcggcgac cagcgcaagc
      841 gcgggcacca tcggccagcg gtacaaagcc gcgtcgtgca ggtgcgcccc cggcgtgctg
      901 ccgcggtaga tgtcgacgac cttgacgagc cggtgggtgg gcacgaacac gtcctcgacg
      961 acgacgtcgt tggagccggt ggcccgcatg ccatcggtgt gccagacgtc gacaatccgg
     1021 atgtcgtcgg cgggaagcag cgccagaacc ggatagatgg cgtcgtccgg cccgcacagc
     1081 gccccgacga tcgcccagtt cccgtccatc acgccggtgg cccaggacca gcgcccgttc
     1141 agccgcacac cgccgtcgac cgggacggcg cggccggtgg gcgccaacgg cgcaggcgcg
     1201 aggaacggcc gggtcgcgaa cgcctcggcc tgggcctgct cgtcgaacag ggccagcatc
     1261 cagttgtgca gcgtgtagaa ggccgcggtc cacgcggtcg aggcgcagcc gtgcgccatc
     1321 cggcgcaccg ggtccaagat cgcgcggaac tccgcttgtt gaccgccgta ccgcgccggt
     1381 ttcaggagat cgatgaagcc ggactccttg agttcggcga cggtggccgc aggcagccgc
     1441 cggagttcct cggcttcctg cgctcgctgt gcgagccgct cgacaaatgc gtcgctgata
     1501 ccggcgtccg tcatggagcg cagcctacgg aggtggcgcg cccggcaggc tgcccacttc
     1561 cagaccggcc agctcgctgg tgccctcgaa caccacccgt ccgaactcgc gcacggtgca
     1621 acggagtcga ccggccaggt gctcgaagtc ggtgtcgaca ttgcgtctct ccgcggctag
     1681 cggtacgggc aggacgtgcg gttccaggtg tgtgccatcc ccgtccaggt cgatctggta
     1741 gcgcagtccc cgtgctcgta cggtccagcg cgcgtcgctg acgtgcgagt gcaccagcgc
     1801 cggtggcgtc acgcggatca cgcggtcacc gagccgcaca accaccccgg cgacgtcccg
     1861 gcccagtggc cccagcttga gaaggccacc cgagaaggcg accgagacgt cgtcaccgcc
     1921 gaagtcgtgc gcctgacccc accaccaccg ctcaggaaag cccgcgcccc agttccgttc
     1981 ggagtaccac cgagcctcgt tgaaggacca gctcgcttcg ctgaattcga cggtcccgct
     2041 tgattttcca ccgagacggt agggatgcca gtactggttg aggaaaggga tcgacgaaaa
     2101 gatgccacca ccggcgaacg ccttgggcca catgaacgga tcgtcgaacc gcaggtcgag
     2161 gtggacgtcg tcaaggtcga ttcggagccg atcggcgctg gcgttcaaag agcccgtcgg
     2221 gtcggtaccg gcgcgaacgg tgaagcggga ttgttccgtt tcggcatcgt ccaacgctgc
     2281 cgaccgcacg atgccg
//
LOCUS       DASWBF010000438         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_964274,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000438 DASWBF010000000
VERSION     DASWBF010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964274"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VF874_16120"
     CDS             complement(<1..285)
                     /locus_tag="VF874_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HEX9177527.1"
                     /translation="MWIGWLELDLLLGDVHSLKEKRSVVRPLVAELRRRFSVSAAETG
                     AMELYRRAGVGVAVTAADRAHVIEVLDAAERLVAARPEMALLSARRGVHRS"
     gene            complement(295..801)
                     /locus_tag="VF874_16125"
     CDS             complement(295..801)
                     /locus_tag="VF874_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177528.1"
                     /translation="MVAAALLAGVAACGNDTHPAASESATATASPSSMIAGPEDTPLP
                     PPEALTDVLYRLADTSVPGDQKVGLVEDAAANDAEKLDKFGKALQDSGYTPVGFTAEN
                     IAWATNPPGSVTADVTVHSQNRAMTNGFTFPMEFKPLPQGGWQLSQTTADILLTYNQN
                     PATPTPTG"
     gene            complement(840..1331)
                     /locus_tag="VF874_16130"
     CDS             complement(840..1331)
                     /locus_tag="VF874_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177529.1"
                     /translation="MSLKSLTTGVAAATLVGAAAVGVTSVASVPITVSPAITPVVFGA
                     PLPLDSDPALTGEFTGILNQLANSGSINGNLIDGGVGIIEARTADQLLKNAAAKGYLP
                     LNISVDPNSITESGNTASATVTASGPALAPTTQTVTFVNNGGWKLSKGSATALLQAAM
                     ASG"
     gene            1477..3294
                     /gene="arc"
                     /locus_tag="VF874_16135"
     CDS             1477..3294
                     /gene="arc"
                     /locus_tag="VF874_16135"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEX9177530.1"
                     /translation="MSESERSDFGASMSSDDAAELEALRREAAALREQLETTLSAQGG
                     LRSARDVHQLEARIDSLASRNSKLMETLKEARQQLLALREEVDRLGQPPSGYGVLLSV
                     QEDDTVDVFTSGRKMRLTCSPNIDVQSLKKGQTVRLNEALTVVEAGHFEAVGEISTLR
                     EILSDGHRALVVGHADEERIVWLAEPLLATEDMAPEVADVLDDLDRPRRLRPGDSLLV
                     DTKAGYAFERIPKAEVEDLVLEEVPDVSYSDIGGLTRQIEQIRDAVELPFLHKELYRE
                     YLLRPPKGVLLYGPPGCGKTLIAKAVANSLAKKMAEVRGDDSREAKSYFLNIKGPELL
                     NKFVGETERHIRLIFQRAREKASEGTPVIVFFDEMDSIFRTRGTGVSSDVETTVVPQL
                     LSEIDGVEGLENVIVIGASNREDMIDPAILRPGRLDVKIKIERPDAEAAQDIFSKYLT
                     ENLPVHADDLAEFDGDRTACIQAMIVKVVDRMYAEIDDNRFLEVTYANGDKEVMYFKD
                     FNSGAMIQNVVDRAKKYAIKSVLETAQRGLRIQHLLDSIVDEFAENEDLPNTTNPDDW
                     ARISGKKGERIVYIRTLVTGKSSSASRAIDTESNLGQYL"
     gene            complement(3296..3925)
                     /locus_tag="VF874_16140"
     CDS             complement(3296..3925)
                     /locus_tag="VF874_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177531.1"
                     /translation="MDANDVPTPPADVFGKRYGEVLLVHFGGSGPEAIVFNSFPLNDC
                     PQELWSKLDTEAIARENDAPMVLLNGPRYWLMSHIEKAPGAEQEKTSFGGIEMLRQAT
                     VQLSSMNPAPYTVNRVNRKALFTFDAGRPVFELVDPDGRRWVMQTWSQTVDAALSLED
                     LPGLASRLNLPAGWAYESRTLTSPLRVDTTTQDAQVTQDDFANSYSLQT"
     gene            complement(3936..4601)
                     /locus_tag="VF874_16145"
     CDS             complement(3936..4601)
                     /locus_tag="VF874_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9177532.1"
                     /translation="MSTTRRADSRRTYDNSRRRADAEGRQRRIVDAATALFVEQGFGG
                     TSIDQIAAAADVSPQTVYATYGSKAAVLSRAIDVAVVGDYEAASLADRMPVLADMSSK
                     QHHLYFATAARFVRALHERVVPLMRVMLQAAATDPGLEELRMRLTHEIRADCEVWVAQ
                     LGPALRRGLTKARAADVMVTIQSPYIYSMFTVDLGWSPDQYERWLAHALPHMLLRPEL
                     LSG"
BASE COUNT          839 a         1537 c         1575 g          793 t
ORIGIN      
        1 gctgcggtgc accccgcgcc gagccgacag caatgccatc tccggccgtg ccgccaccag
       61 ccgctcggcc gcatcgagca cctcgatcac gtgcgcgcgg tcggccgccg tcaccgccac
      121 accgactccg gcgcggcgat acagctccat cgcgccggtt tcggccgccg acacggaaaa
      181 ccggcggcgc agctcggcga ccaggggccg caccacggaa cgcttctctt tcagcgagtg
      241 cacgtccccg agcagcaggt cgagctccaa ccacccgatc cacatcaggc cggctcaacc
      301 cgtcggtgtc ggcgtcgccg gattctggtt gtaggtcagc aggatgtcgg ccgtcgtctg
      361 cgagagctgc cagccgccct gcgggagggg cttgaactcc atcgggaacg tgaacccgtt
      421 cgtcatggcc cggttctggc tgtgtacggt cacgtccgcc gtcacgctgc ccggcgggtt
      481 ggtggcccag gcgatgttct cggcggtgaa gcccaccggt gtgtagccgc tgtcctgcag
      541 agctttgccg aacttgtcga gcttctccgc gtcgttggcg gcggcgtcct cgacgaggcc
      601 gaccttctgg tcgccgggca cggaggtgtc ggcgagtcgg tacagcacat cggtgagcgc
      661 ctcgggcggc ggcagcgggg tatcctcagg cccggcgatc atcgacgacg gactggccgt
      721 ggccgtggcg gactccgacg ccgcggggtg ggtgtcgttg ccgcatgcgg ccacgcctgc
      781 caacagtgcg gcagcgacca cggcgacagc cgccggacgc cgccgcaccg tggaacgaat
      841 caacccgagg ccatcgcggc ctgcagcagc gcggtggcgg accccttgga aagcttccag
      901 ccgccgttgt tgacgaacgt gacggtctgc gtggtgggtg cgagggctgg accggacgcc
      961 gtcaccgtcg cgctggccgt gttgcccgac tccgtgatgc tgttggggtc cacggagatg
     1021 ttcagcggca ggtagccctt cgcggccgcg ttcttcagca gctgatcggc ggtgcgggcc
     1081 tcgatgatgc cgacaccgcc gtcaatcagg ttcccgttga tcgatccgga gttggcgagc
     1141 tggttgagga tgccggtgaa ctctccggtg agagccggat cgctatccag tggaaggggt
     1201 gcgccgaaca ccaccggcgt gattgccggc gacacggtta tagggacaga tgcaacggag
     1261 gtcacaccga cggccgcagc accgaccaga gtggccgctg cgacgccagt ggttagggat
     1321 ttcaggctca caaacgtcct ttcgttccgc ccgacgaaaa cgtaacgcta gcagttcggc
     1381 ggtgcgccag gtttttagac cgcacgcgaa tgggaattcg ccggtagcgt tggtgtatcc
     1441 accgcaccaa cttccggtgc gggagaggag cgcaacatga gtgagtcaga gcgttcggac
     1501 ttcggagcgt cgatgtccag cgacgacgct gccgaactag aagcgctccg tcgcgaggcc
     1561 gcagctcttc gggaacagct tgaaacgacc ctcagcgccc agggcggact gcgtagcgcc
     1621 cgcgatgttc accagctcga agcgcgcatc gactcgctcg ccagccggaa ctcgaaatta
     1681 atggagaccc ttaaagaggc acgccagcag ttgcttgcgc tgcgcgaaga ggtcgatcgg
     1741 ctcgggcaac cgcccagcgg gtacggcgtc ctgctctctg ttcaggagga cgacaccgtc
     1801 gacgtcttca cctcgggtcg caagatgcgt ctcacctgct ctcccaacat cgacgtgcag
     1861 tcgctcaaga agggccagac ggtccggctg aacgaggcgc tcaccgtcgt cgaggccggt
     1921 catttcgagg cggtcggcga gatcagcacg ctgcgcgaaa tcctttccga cggccaccgc
     1981 gcgctcgtgg tgggtcacgc cgacgaggag cgcatcgtgt ggctggccga gccgctgctg
     2041 gcgactgagg acatggcacc cgaggtggcc gatgtgctcg acgacctcga ccggccccgc
     2101 aggctgcggc cgggcgactc gctgctggtg gacaccaagg ccggatacgc gttcgagcgc
     2161 atccccaagg ccgaggtcga ggacctggtt ctcgaagagg ttcccgacgt cagctacagc
     2221 gacatcggcg gcttgactcg ccagatcgag cagatccgcg atgcggtgga gctgccgttc
     2281 ctgcacaagg agctgtaccg cgagtacctg ctgcggccgc cgaagggtgt gctgctgtac
     2341 gggccgcccg gatgcggcaa gacgctgatc gccaaggccg tcgccaactc gctggccaag
     2401 aagatggccg aggttcgcgg cgacgactcg cgcgaggcga agtcctactt cctcaacatc
     2461 aagggtcccg aactgctcaa taagttcgtc ggcgaaaccg aacgccacat ccggctgatc
     2521 ttccagcgcg cccgcgagaa ggcgtcggaa ggcacaccgg tgatcgtgtt cttcgacgag
     2581 atggactcga tcttccgcac ccgcggtacc ggggtttcgt ctgacgtcga gaccacggtc
     2641 gtgccgcagc tgctgtcgga gatcgacggc gtcgaggggt tggagaacgt catcgtgatc
     2701 ggcgcgtcca accgcgagga catgatcgac ccggcgatcc tgcggccggg ccggctggac
     2761 gtcaagatca agatcgagcg gccggatgcc gaagcggctc aggacatctt cagcaagtac
     2821 ctcaccgaga acctgccggt gcacgccgac gacctcgccg agttcgacgg cgaccgcacg
     2881 gcttgcatcc aggcgatgat cgtcaaggtc gtcgaccgga tgtacgccga gatcgacgac
     2941 aaccggttcc tcgaagtcac ctacgccaac ggcgacaaag aggtcatgta cttcaaggac
     3001 ttcaactccg gcgcgatgat ccagaacgtc gtcgaccggg ccaagaagta cgcgatcaag
     3061 tcggtgttgg agactgcgca gcgcggtctg cgcatccagc acctgctcga ctcgatcgtc
     3121 gacgaattcg ccgagaacga ggacctgccc aacaccacga atcccgatga ctgggcgcgg
     3181 atctcgggca agaagggcga gcggatcgtc tacatccgca cgctggtcac cggcaagagc
     3241 agcagcgcgt cccgcgccat cgacaccgag tcgaacctgg gccagtacct gtagatcagg
     3301 tctgcagcga gtagctgttc gcgaagtcgt cctgggtgac ctgggcgtcc tgcgtggtgg
     3361 tgtctacccg cagcgggctg gtcagcgtcc gcgattcgta ggcccatccg gcgggaaggt
     3421 tgagccggct cgcgagaccc ggcaagtcct ccagggacag ggcggcatcg acggtctggc
     3481 tccaggtctg catcacccag cgccgaccgt cggggtcgac gagttcgaac accggccggc
     3541 cggcgtcaaa cgtgaagagg gccttgcggt tgacccggtt gacggtgtac ggggcggggt
     3601 tcatcgacga cagctgaacc gtggcttgcc ggagcatctc gatgcccccg aagctcgtct
     3661 tctcctgctc agcaccggga gccttctcga tgtggctcat cagccagtag cgggggccgt
     3721 tgagcaggac catcggggcg tcgttctcac gggcgatggc ctcggtgtcg agcttcgacc
     3781 acaattcctg cgggcagtcg ttcagcggga agctattgaa gacgatggcc tcgggacccg
     3841 agcctccgaa gtgcacgaga agcacttcgc cgtaacgctt cccgaacacg tcggccgggg
     3901 gtgtaggcac gtcgttggcg tccacggtcg tgaagttacc ccgacagaag ctccggccgc
     3961 agcaacatgt gcggcaacgc gtgggcaagc caccgttcgt attggtccgg cgaccacccc
     4021 aggtccacgg tgaacatcga atagatgtag ggcgactgga ttgtcaccat cacgtccgcg
     4081 gcccgagcct tggtcaggcc gcgtcgcagc gccgggccca gttgtgcgac ccacacctcg
     4141 cagtcggcac gaatctcgtg ggtcagcctc atccgcagtt cttccaagcc tgggtcggtg
     4201 gcggccgcct gcaacatcac ccgcatcagc ggtacgaccc gttcgtgcag tgcgcgcacg
     4261 aagcgcgcag ctgtcgcgaa gtacaggtgg tgctgcttgc tcgacatgtc ggccagcacc
     4321 ggcatccgat cggccaacga ggccgcctcg tagtcgccca ccaccgccac gtcgatggcg
     4381 cgggacagca cggcagcctt cgagccgtag gtcgcgtaga cggtctgcgg ggaaacatcg
     4441 gccgcggcag cgatctggtc gatcgacgtc ccgccgaagc cctgctcgac gaacaacgcc
     4501 gtcgccgcgt cgacgatcct gcgctgacgc ccctctgcgt cggcacggcg gcgactgttg
     4561 tcgtacgtgc ggcgggagtc cgcccgacga gtggttgaca cgagtatgcg tccttccata
     4621 tagttaactt tattgaatag agttacatct atctaaccat actacggagg ttgccatggc
     4681 aacccgtgtg atggcagggc gtgcggttcc acctgttccg ttggtgagac tgactgagcg
     4741 ccta
//
LOCUS       DASWBF010000439        11794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_302705,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000439 DASWBF010000000
VERSION     DASWBF010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11794
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302705"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VF874_16150"
     CDS             <1..670
                     /locus_tag="VF874_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9177533.1"
                     /translation="VGFVVTFLLGGLSGVLLASPPLDFHVTDSYFVVAHFHYVLFGTI
                     VFATFAGIYFWFPKMTGRMLDERMGRLHFWLTLIGFHTTFLVQHWVGNEGMPRRYADY
                     LPTDGFETLNIVSTIGSFILGASMIVFTWNVFRSWRYGAPVTVDDPWGYANSLEWATS
                     CPPPRHNFYELPRIRSERPAFELHYPHMIERMRAESHVGRYATVGESEGMPDRSGQAA
                     PERG"
     gene            675..1913
                     /gene="serB"
                     /locus_tag="VF874_16155"
     CDS             675..1913
                     /gene="serB"
                     /locus_tag="VF874_16155"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX9177534.1"
                     /translation="MPNAGAAVSVLITVTGVDQPGVTSALFGVLSRYDVELLNVEQVV
                     IRSRLTLGVLVACAEDIADGASLREDVERVIHGVGLEVSIERSRDLPIIVDPSTHTIV
                     VLGRPVTARAFSALATELASLGVNIDFIRGVSDYPVTGLELRVSVPGERAAELPTALA
                     RVATEEKVDVALEDTSLERRAKRLIVFDVDSTLIQQEVIEMLADRVGARDAVAAITQA
                     AMRGELDFAESLRQRVATLKGLPASVLDDVGDELELTPGARTTLRTLRRLGFHCGVVS
                     GGFRQVIEPLAHELMLDFVAANELEIVDGVLTGNLVGPIIDRPGKAKALKDFAQQAGV
                     PLEQTVAVGDGANDIDMLAAAGLGVAFNAKPALREVADASLSHPYLDTVLFILGVTRG
                     EIEAADAVDGTLRRVDIPPN"
     gene            2028..2489
                     /locus_tag="VF874_16160"
     CDS             2028..2489
                     /locus_tag="VF874_16160"
                     /inference="COORDINATES: protein motif:HMM:NF042550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6636 domain-containing protein"
                     /protein_id="HEX9177535.1"
                     /translation="MKVRIGLAVSITLAAAAFSLAPIAQADDYTNYEFGDPSKNIACM
                     LRLLPSGRPDDPDNTVQCDIDKHDWVGPQSGPGPCPQDEGYIFMLRQQGTPEMTCLNG
                     SVLPTIYTVLEYGQSRTAGAITCDSESSGMTCTNTATGHFFRVSRASYQLG"
     gene            2614..3510
                     /locus_tag="VF874_16165"
     CDS             2614..3510
                     /locus_tag="VF874_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9177536.1"
                     /translation="MRHDGRVVEFGGQERSDRGISTAIPAPDPDLLIDFHKVSLRRGG
                     RTLVGPVTWSVELDERWVVIGPNGAGKTSLLRIAAAMEYPSSGTAYVLGERLGRVDMS
                     ELRSRVGLSSSALSQRVPDGESVRDLVVSAGYAVLGRWRETYDDVDYEQAIDMLESVG
                     AEHLADRRYDTLSEGERKRVLIARSLMTDPELLLLDEPAAGLDLGGREELVARLADLA
                     ADPDAPAIVLVTHHVEEIPPGFSHGLVLSEGEVVAAGLLPDVLTADNLSKAFGQSIAL
                     DLIDGRYFARRVRTRAAHRRRS"
     gene            3507..4304
                     /locus_tag="VF874_16170"
     CDS             3507..4304
                     /locus_tag="VF874_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9177537.1"
                     /translation="MSTQMTPRPAATVMLLRDAPVGLEVFLLERHSRMDFAPGTTVFP
                     GGGVDDRDREADIAWTGPEPSWWAQRMAVDEELALALVCAAVRETFEECGVLLAGPDE
                     GSVVGDATVYHGAREQLASRELAFSDFLRSQRLVLRADLLRPWANWVTPAKGHRRRYD
                     TYFFVAALPEGQQADDRTSEAVSAGWSLPAEAVEDFAAGRTVLLPPTWTQLDTLPKFG
                     NSVDDVLAHDREIVAMRTEVIGSEGNWSLEFFDSERYNKLLGGRPWS"
     gene            4301..5035
                     /locus_tag="VF874_16175"
     CDS             4301..5035
                     /locus_tag="VF874_16175"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9177538.1"
                     /translation="MNEFVSVYVSDERPGVGTIVLSRPPENTLTRQACREIVAVAAEV
                     GRREDIAAVILFGGHEIFSAGDDLAELRTLGPAEADGFARLRQDAAEAVAAIPKPTVA
                     AITGHALGSGLSLALAADWRVSGDNARLGFTEVLAGLIPGGGALQRLPALVGVSKAKD
                     LAFSGRFVDAREALAMGLLDEIVAPDAVYDAAAEWVQRFVGVSARALAAAKAAINDGT
                     PDAERRLYADAFATAERRFVASDDGR"
     gene            5079..6047
                     /locus_tag="VF874_16180"
     CDS             5079..6047
                     /locus_tag="VF874_16180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9177539.1"
                     /translation="MTSTSEGTPQPHATAEQVEAALHDSKLAQVLYHDWEAESYDDKW
                     SISYDKRCVDYARDLFDATVPGEEQRRLPYDRALELGCGSGFFLLNLIQAGVARRGSV
                     TDLSPGMVRVATRNGENLGLEIDGRVADAEGIPYDDDTFDLVVGHAVLHHIPDVELSL
                     REVVRVLKPGGRFVFAGEPTSVGDRYARTLSTATWRAVTNATKLPGLSGWRRPQAELD
                     ESSRAAALEAIVDLHTFDPADLERMARNAGAVEISTATTEFTAAMLGWPLRTIEAAVP
                     PERLGCNWARFAFSSWITLGWIDDNLWRRVIPKGWYYNVMVTGVKP"
     gene            5984..7204
                     /locus_tag="VF874_16185"
     CDS             5984..7204
                     /locus_tag="VF874_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9177540.1"
                     /translation="MAPRDPEGLVLQRDGDRGQALIFTLDDVRYLQSSAGADALAQVA
                     TCPLTDATRIADVSAARTRFGDRAGVLVETVLLRRRAAAKLGDAGDWLFTDEALQQAT
                     AAPVAAHRARRLTGSTVHDVTCSIGTELAVLRRTADVLGSDIDPVRLAMARHNVPGVP
                     LIRADALRPVSRGTVVLADPARRSGGRRVFAPRDFSPPLDALLDTYRDRNIVVKCSPG
                     IDFARLEFRGEVEVVSLSGEVREACLWSPGLTEPRVSRRATVLGGDGSVEQVTDAPPD
                     DCPVGPAGRWIIDPDGAVVRAGLVRQYAARYGLWQLDPKIAYLSGDGLPPATRGFEVL
                     EQIPFGERPLRQALAEMDCGAVEILVRGVDVDPAVLRRRLRLRGARPLSVVITRIGAA
                     SSARATAFICRPSR"
     gene            7248..7946
                     /locus_tag="VF874_16190"
     CDS             7248..7946
                     /locus_tag="VF874_16190"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEX9177541.1"
                     /translation="MTRSLRAAALLAAAILIGSGGSAVAQAQSPCADFGGTLDGDTCR
                     VHTATGAYIVDMRFPVDYADPAPMIDYLKQTRDGFVNVSQMPGSTNLPYSLDITSDEY
                     RSGRAPGGTQSVVLKIYQNVGGAHPLTWYQAYNYNLNTRQPITFDTLFTAGSKPLPVI
                     LPIVQKDIDTQFGSRVTISPGDGLDPSHYQNFALTDDELIFFFGQDELLPSAAGATSV
                     RVPRSAVASMLAPV"
     gene            complement(8032..9156)
                     /locus_tag="VF874_16195"
     CDS             complement(8032..9156)
                     /locus_tag="VF874_16195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005561624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX9177542.1"
                     /translation="MTTAQAEMDGLTIFLCGDVMTGRGVDQILPHPGDPTLREPVVDD
                     ARTYVELAEQANGPIPNPADFSWPWGEALGVLDEFAPDGRLLNLETAVTSGSDFAPYK
                     AIHYRMHPDNVECLTAPRPDVCVLANNHTLDFGTGGLADTLRVLADAGIPCAGAGLDA
                     EEAHRPAVTETDGGRVICIAAGMKSSGIPGSWAATEDRPGVAFLSDMSKRTATQIADR
                     AVALKRAGDVAVASLHWGSNYGYHIDSSQRHFAHHLIDAGIDVVYGHSSHHPRPIEVY
                     RGKLILYGCGDLVNDYEGIGGYQAYRGELRLMYFASVDPASGRLIVLNMVPMREVRMR
                     LQRGACVDVEWMRSTLEDTSRLFGTRVETKADGVLAVRAR"
     gene            complement(9216..10514)
                     /locus_tag="VF874_16200"
     CDS             complement(9216..10514)
                     /locus_tag="VF874_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9177543.1"
                     /translation="MFSRHRTPRRLAALVGAALLLVSGCGNTDTWVDAAPAAGWSAQY
                     ADAANSSYTTTAGAESLKLTWSRSVKGSLFASVALGASNYLAANAQTAAGCSLMVWEH
                     DNNGRQRWCTRLVLGGGFASPLFDKFDNLYVGQPGMMLSFPTTQWVRWRHPVIGMPQT
                     PRFLGPNRLLVVTHLGQVQVVDAHRGEMVGSAVDLVQGVDPTDSQRGLSDCQAAGPGC
                     PVAAAPALSPSAGLIVSGIWQPGAPAATLTALRYRADVGQVLSPEWNSEAITGGVLGS
                     PVFSADGSTVYVNSRDGKLWALDGKDGKPKWSVPLGFSPQTPPSVSPGGLLVSGGGPG
                     AKLVAVRDNGERGDVIWRRDDVTPLCTSSQAGDDIGYTVVRSGDNALALLVFRIGDGR
                     TVNTYPLPQATGWPVGVSVATDRSVITATSDGRAYAFDPA"
     gene            10591..11328
                     /locus_tag="VF874_16205"
     CDS             10591..11328
                     /locus_tag="VF874_16205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9177544.1"
                     /translation="MTTMWGAPLHKRWRGSRLRDPRQAKFLTLASLKWVLANRAYTPW
                     YLVRYWRLLKFKLANPHIITRGMVFLGKGVEIQSTPELAQMDIGRWVHIGDKNTIRCH
                     EGSLRFGDKVVLGRDNVINTYLDIELGDSVLMADWVYVCDFDHRMDDINMPIKDQGIL
                     KSPVRIGPDTWIAAKCTILRGTIVGRGCVLGSHAVVKGHIPDYSIAVGAPAKVVKNRR
                     VAWETSAAERAQLAAALADIERKKASR"
     gene            complement(11405..>11794)
                     /locus_tag="VF874_16210"
     CDS             complement(11405..>11794)
                     /locus_tag="VF874_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9177545.1"
                     /translation="LMLDSPAMGWGIHIDRVEIKDVALPDSMKRSIARQAEAERERRA
                     RVITADGELQASQKLAQAAGVMAASPSSLQLRLLETVVEVAAEKNSTLVLPFPVELLR
                     FLEKSTPRSKSDAETPQVPDDPRSLLP"
BASE COUNT         1885 a         3890 c         4062 g         1957 t
ORIGIN      
        1 cgtcgggttc gtggtgacgt tcctgctcgg tgggctgtcg ggggtgctgt tggccagccc
       61 gccgctggac ttccacgtca ccgacagcta tttcgtggtg gcgcacttcc actacgtgct
      121 gttcggcacg atcgtgttcg cgacgtttgc gggcatctat ttctggttcc cgaagatgac
      181 gggcaggatg ctcgacgaac ggatgggccg gctgcatttc tggctgacgc tgatcggttt
      241 tcacaccacg ttcttggtgc agcactgggt gggcaacgag ggcatgcccc gccgctacgc
      301 cgactacctg cccaccgacg gcttcgagac gctcaacatc gtgtccacca tcggctcatt
      361 catcctgggc gcctcgatga tcgtgttcac ctggaacgtg ttccgcagct ggcgctacgg
      421 cgcgccggtg accgtggacg acccgtgggg ctacgccaac tcgctggagt gggccaccag
      481 ctgcccacca ccacggcaca acttctacga gctaccccga atccgttcgg aacggcccgc
      541 cttcgaactg cactacccgc acatgatcga acgcatgcgc gccgaatccc acgtcggcag
      601 gtatgccacc gtgggcgagt ccgaaggtat gcccgaccga agcgggcagg cagcgccgga
      661 acggggctga tcgcgtgccg aacgccggcg cagcggtctc ggtactgatc actgttacag
      721 gggtcgatca gccaggtgtg acgtcggcgc tgttcggggt gctgtcgcgg tacgacgtcg
      781 agctgctcaa cgtcgagcag gtggtgatcc gcagccgtct gacactgggc gtgctggtgg
      841 cgtgtgccga ggacattgcc gatggcgcgt cactgcgtga agacgtggag cgcgtgattc
      901 acggtgtggg acttgaggtt tcgatcgagc gcagccgcga cctgccgatc atcgtcgacc
      961 cgtcgacgca cacgatcgtc gtgctcggca ggcccgtcac cgcccgcgcg ttcagcgcgc
     1021 tcgctaccga actggcctcg ctgggcgtca acatcgattt cattcgcggc gtctccgact
     1081 atccggtgac cggtctggaa ctgcgggtgt cggtgcccgg cgagcgggcc gctgaactgc
     1141 cgaccgcgct ggcccgcgtc gccaccgagg agaaggtcga cgttgcgctc gaggacacca
     1201 gcctggagcg acgggccaag cggctgatcg tgttcgacgt cgactccacg ctgatccagc
     1261 aggaggtcat cgagatgctg gccgaccgcg tcggggcgcg tgacgcggtt gccgcaatca
     1321 cccaggccgc gatgcggggg gaactcgatt tcgccgaatc gctgcggcaa cgtgtcgcga
     1381 cgttgaaagg cttgcctgcc agcgtgctcg acgacgtcgg cgacgagctg gagctcacgc
     1441 cgggcgctcg gaccacgttg cgcacgttgc gccggctggg ctttcactgc ggcgtggtgt
     1501 ccggcggctt ccggcaggtg atcgagccgc tggcgcacga gttgatgctc gatttcgtcg
     1561 ccgccaacga gctcgagatc gtcgatggcg tgctgactgg caacctcgtt gggccgatca
     1621 tcgaccggcc cggaaaagcc aaggcgctca aggactttgc gcagcaggcc ggtgtgccgc
     1681 tggagcagac tgtggccgtc ggcgacggcg ccaacgacat cgacatgctg gccgcggccg
     1741 ggctgggcgt cgcgttcaac gccaaacctg cgctgcgcga ggtcgccgac gcgtcgcttt
     1801 ctcacccgta tctggacacg gtgctgttca tcctcggcgt gactcgcggt gagatcgagg
     1861 cggccgacgc ggtcgacggg acgctgcgcc gggtcgacat cccgccgaac tgagttgaag
     1921 atccggcctt tcgaggcgaa tttgcgacat gccggttggt ggcccgcgca taataagcgc
     1981 acaaagaccc cagccgcgaa cagggctgac taaggaagaa cgacatcatg aaagtacgga
     2041 tcggtctggc ggtctcgatc acgctcgcgg ctgcagcgtt ttctctcgcg cctatcgcgc
     2101 aggccgacga ctacacgaat tacgagttcg gggacccgtc gaaaaacatc gcctgcatgc
     2161 ttcgtttgct cccttccggt cgacccgatg atccagacaa caccgtccag tgcgatatcg
     2221 ataagcacga ctgggtggga ccacagtcgg gcccggggcc ctgccctcaa gacgagggtt
     2281 acatcttcat gctccgtcaa cagggcacgc cagaaatgac ctgcctcaac ggttcggtgc
     2341 ttccgaccat ttacacggtg ctcgagtatg gccaatcacg aacggcgggc gcgatcacct
     2401 gcgacagcga gtcctccggt atgacatgca ccaacaccgc caccgggcat ttcttccggg
     2461 tgtcgcgcgc ctcctatcag ctgggctgac ggtcgccgac gcatctcgcg ccttctctcc
     2521 cgcgagcaga cagaaaagtc cccgcgctct cggcatgtcg ggtgcatttg catctgctgc
     2581 tcgcgcaggg tggtgactag cgcacatcgg ccgatgcggc acgatggtcg ggtggtcgaa
     2641 ttcggcgggc aggagcgtag cgaccgggga atcagcacag ccattcccgc gcccgatccc
     2701 gacctattga tcgactttca caaggtttcg ctgcggcgcg gcggtcgaac tctcgtcggc
     2761 cccgtcacct ggtctgtgga actcgacgag cgctgggtgg tgatcggccc caacggcgcc
     2821 ggcaagacgt cgctgctgcg gatcgccgcg gcgatggagt acccgtcgtc gggcaccgcg
     2881 tatgtgctcg gtgaacggct gggccgagtg gacatgtcgg aactgcgctc gcgggtgggg
     2941 ctgagcagct cggcgctgtc gcagcgggta cccgacggcg agagcgtacg cgacctggtg
     3001 gtgtccgcag gctatgcggt actgggccgt tggcgagaga cctacgacga cgtcgactac
     3061 gagcaggcca tcgacatgct ggaaagcgtc ggcgccgagc acctggccga ccgaaggtac
     3121 gacaccctgt ccgagggcga acgcaagcgg gtgctgatcg cgcggtcact gatgaccgac
     3181 ccggagctac tgctgctcga cgagccagcc gcggggctgg acctgggcgg ccgcgaggaa
     3241 ctggtggcgc ggctggccga cttggccgcc gacccggacg ccccggcgat cgtgctggtc
     3301 acccaccacg tcgaggagat cccgcctggc ttcagccacg gtctggtgct gtcggaagga
     3361 gaagtggtcg ccgcgggcct gctgcctgac gtgctgaccg cagacaacct gtccaaggcg
     3421 ttcggccagt ccattgcgct cgacctgatc gacggccgct acttcgccag gcgtgtccgc
     3481 acccgtgccg cacacaggag gcgctcatga gcacgcagat gacgccgcgt ccggccgcga
     3541 ccgtaatgct gttgcgcgac gctccggtgg gcctggaagt ctttctgctg gaacgacatt
     3601 cgcggatgga cttcgccccc gggacgacgg tgtttcccgg cggcggcgtc gatgaccgcg
     3661 accgggaagc cgacatcgct tggaccggac cggagccgag ttggtgggcg cagcggatgg
     3721 ccgtcgacga ggagctggcg ctggcgctgg tgtgcgccgc ggtgcgcgaa acgttcgagg
     3781 agtgcggggt gctgctcgcc ggtcccgacg agggcagcgt ggtcggcgac gcaacggtct
     3841 accacggtgc gcgtgaacag ctcgccagca gagagctggc gttcagcgac ttcttgcgca
     3901 gccagcgcct ggtgctgcgc gcggacctgc tgcggccgtg ggcgaattgg gtcaccccgg
     3961 cgaaggggca ccggcgccgc tacgacacct actttttcgt cgcggcgctg ccggaaggcc
     4021 agcaggctga cgaccgaacc agcgaggcgg tgtcggcagg atggtcgttg ccggccgagg
     4081 cggtcgagga cttcgccgca ggtcgcacgg tcctgctacc gccgacatgg acgcagctgg
     4141 acacgctgcc gaaattcggc aactccgtcg acgacgtcct cgcccacgac cgcgagatcg
     4201 tcgcgatgag aacggaagtc attggcagcg agggtaattg gagcctggag ttcttcgact
     4261 cggagcgcta caacaagctg ctcggcgggc gaccctggtc gtgaacgagt tcgtcagcgt
     4321 ctacgtcagc gacgaacgtc ccggcgtcgg cacgatcgtg ctgtcacgcc cgcccgaaaa
     4381 cacgctgacc cggcaggcct gccgcgagat cgtcgcggtg gctgccgagg tcggccgccg
     4441 cgaggacatc gccgccgtca tcctgttcgg tggccacgag atcttttcgg cgggcgacga
     4501 cctcgccgaa ctgcgcacgc tgggccctgc cgaagccgac ggcttcgcgc ggttgcgtca
     4561 ggacgcggcg gaggccgtcg ccgcgattcc gaaaccgacg gtcgccgcga tcaccggcca
     4621 cgccctcggc agcgggctgt cgctggcgct ggccgccgac tggcgcgtca gtggtgacaa
     4681 cgcgaggctg ggcttcaccg aagtcctcgc cgggctgatt cccggcggtg gtgcgctgca
     4741 gcggctgccc gcgttggtcg gggtgagcaa ggctaaagat ttggcgttct ccgggcggtt
     4801 cgtcgacgcc cgggaggcgc tggcgatggg tctgctcgac gagatcgtgg cacccgacgc
     4861 ggtctacgac gccgccgcgg aatgggtgca gcgcttcgtc ggcgtttcgg cgcgggcact
     4921 ggccgccgcg aaggctgcga tcaacgacgg caccccggac gccgagcgac ggctgtacgc
     4981 cgacgcgttc gccaccgctg agcggcggtt cgtggcatct gacgacggcc gatgagcgct
     5041 tgcgcgaaga ggagaaggca ccgataggct gccccgttat gacgagcaca tccgaaggga
     5101 cgccccagcc gcacgcgacg gccgaacagg tcgaggccgc cctgcacgac agcaagctcg
     5161 cgcaggtgct ctaccacgac tgggaggccg agagctacga cgacaagtgg tcgatctcct
     5221 acgacaagcg ctgcgtcgac tacgcgcgcg acctgttcga cgcgaccgtg cccggcgagg
     5281 agcaacgccg gttgccctac gaccgtgcgc tggaactggg ctgcggcagc ggctttttct
     5341 tgctgaacct gatccaggcc ggggtcgcgc ggcgcggttc ggtgaccgac ctgtcgccgg
     5401 gcatggtgcg cgtcgccacc cgtaatgggg agaacctcgg cctggagatc gacggtcgcg
     5461 tcgccgacgc cgaaggcatc ccctatgacg acgacacctt cgacctggtc gtggggcatg
     5521 cggtgctgca tcacattccc gacgtcgagc tgtcgctgcg ggaagtggtc cgggtgctca
     5581 agccgggcgg gcggttcgtg ttcgccggtg agcccaccag tgtgggtgac cgctatgccc
     5641 gcacgttgtc cacggcgacc tggcgtgcgg tgacgaacgc gacgaagctg ccggggctga
     5701 gcggctggcg gcgcccgcag gccgagctcg acgagtcgtc acgggcagcg gcgctggagg
     5761 cgatcgtcga cctgcacacg ttcgatccgg ccgacctgga gcggatggcg cgcaacgccg
     5821 gcgccgtcga gatctccacc gccaccaccg aattcaccgc cgccatgctc ggttggccgc
     5881 tgcggaccat cgaagccgcg gtgccacccg agcgcctcgg ctgcaactgg gcgagattcg
     5941 cgttcagcag ctggatcacg ctgggctgga tcgacgacaa cctgtggcgc cgcgtgatcc
     6001 cgaagggctg gtactacaac gtgatggtga ccggggtcaa gccctgattt tcacgctcga
     6061 cgacgtccgc tatctgcagt cgtcagcggg cgccgacgca ctcgcgcagg tggcgacctg
     6121 cccgctgacc gatgccaccc gaatcgcgga tgtttccgca gcgcggacca ggttcggcga
     6181 ccgcgccggc gtgctggtgg agacggttct gctgcgccgc cgcgccgcgg ccaagctcgg
     6241 cgacgcgggg gactggctgt tcaccgacga agctctccag caagcgaccg ctgcccccgt
     6301 ggccgcacac cgggcacgtc ggctgaccgg ctcaacggtg cacgacgtca cctgctcgat
     6361 aggcaccgag ctagcagtgc tgcgccggac agccgacgtg ctgggcagcg acatcgaccc
     6421 ggtccggctc gcgatggcac gccacaacgt tcccggagtg ccgctgattc gcgcggacgc
     6481 gcttcgtccc gtcagtcgcg gtaccgtcgt gctggccgac ccggcgcgcc gcagcggggg
     6541 ccgtcgggtc ttcgcgccgc gcgatttcag cccgccactg gacgcgctgc tggacaccta
     6601 ccgcgaccgc aatatcgtcg taaagtgttc ccccggaatc gatttcgcac gtctggaatt
     6661 ccgtggggag gtcgaggtcg tctcgctgtc cggcgaggtg cgtgaggcgt gcctgtggtc
     6721 gcccggtttg acggagccac gggtgtcgcg gcgagccact gtgctcgggg gcgacggatc
     6781 cgtcgaacag gtcaccgacg cccctccaga cgactgcccg gtcgggccgg ccggccggtg
     6841 gatcatcgac cccgacggag cggtggtgcg cgcgggcctg gtccgtcagt acgccgccag
     6901 gtacgggctg tggcagctag acccgaagat cgcctacttg tccggcgacg gattgccgcc
     6961 cgcgacgcgc ggattcgagg tcctcgagca aatcccgttc ggcgagcgac cgttacggca
     7021 ggcgctcgcc gagatggact gcggcgccgt ggaaatcctg gtgcgcggcg tcgacgtcga
     7081 cccggcggtg ctgcggcggc ggctgcggct gcgcggcgca cgacctctgt cggtggtgat
     7141 cacccggatc ggcgcggcgt cgagtgcgcg ggcaactgca ttcatttgcc gcccgtcgcg
     7201 gtgagcgcac aggtaccctg gcgctcgaca cgtttcaagg acggtgcatg acacgctcac
     7261 tcagggcggc cgcgctactg gctgccgcga tattgatcgg gtcgggggga agcgccgtgg
     7321 cgcaggcaca atcgccgtgc gcggacttcg gcgggacgct cgacggcgac acgtgccggg
     7381 tgcacaccgc caccggcgcc tacatcgtgg atatgagatt ccccgtcgac tacgccgacc
     7441 cggcgccgat gatcgactac ctgaagcaga cgcgtgacgg gttcgtcaac gtctcgcaga
     7501 tgccgggttc gaccaatctg ccgtactcgc tggacatcac gtctgacgaa taccgttcgg
     7561 gccgtgcgcc gggcggcacc cagagtgtgg tgttgaagat ctaccagaac gtcggcgggg
     7621 ctcacccgtt gacctggtac caggcgtaca actacaacct caacactcgc cagccgatca
     7681 cattcgacac gctgttcacc gcgggcagca agccgctgcc ggtgatcctg ccgatcgtgc
     7741 agaaggacat cgacacgcag ttcggcagcc gcgtcacgat ctcgcccggc gacggtctcg
     7801 acccgtcgca ctatcagaac ttcgcgctca ctgacgacga gctgatcttc ttcttcggcc
     7861 aagacgagct gctgccgtcg gcggccggtg ccacctcggt ccgtgtgccg cggtcagccg
     7921 tggcgtcgat gctcgcgccg gtctaatccg gcgcgacgaa acagcatctg gtgacgctgt
     7981 gagcggacgc gccgcagcag cggcgggatg ttccggcgac gctgatccgc gctaccttgc
     8041 gcgcactgcg aggacgccgt cggccttggt ctcgacgcga gtgccgaaga ggcggctggt
     8101 gtcttcgagc gtggaccgca tccactccac gtcgacacac gcgccgcgtt gcagccgcat
     8161 ccggacctcc cgcatgggca ccatgttcag gacgatcaac cggccgctgg cagggtcgac
     8221 cgacgcgaag tacatcagcc gcagctcacc ccggtaagcc tggtagccgc cgataccctc
     8281 gtagtcgttg acgagatcac cgcagccgta caagatcagc ttcccccggt acacctcgat
     8341 cggccgcgga tgatgtgacg agtgcccata cacgacatcg atacccgcat cgatcaggtg
     8401 atgcgcaaag tggcgctggc ttgagtcgat gtgatagccg taattggagc cccagtgcag
     8461 cgacgcgacg gcaacatcgc cggcgcgttt caacgccacg gcacgatcgg ctatctgtgt
     8521 ggcagtgcgc ttggacatgt cggaaaggaa cgcgactcct ggccggtcct cggtggcagc
     8581 ccaactgccc ggaatgccac tggatttcat gcctgccgca atgcagatca cgcgaccgcc
     8641 atcggtctcg gtgactgcgg ggcggtgcgc ttcttcggcg tcgagtccgg cgccggcgca
     8701 ggggattccg gcgtcggcga gcacgcgcag ggtgtcagcg agtccgccgg tcccgaagtc
     8761 gagggtgtgg ttgttggcca ggacgcacac atcgggacgg ggggcggtca ggcattcgac
     8821 gttgtccggg tgcatgcggt aatggatggc cttgtacggc gcgaaatcgc taccactggt
     8881 gacagcggtt tccaggttta gcagccgccc atcgggcgcg aattcgtcga ggacaccgag
     8941 cgcttccccc cacggccagc tgaaatccgc tgggttaggt atcgggccgt tggcctgctc
     9001 ggcaagctcc acgtaggtgc gagcgtcatc gacgactggc tcgcgcagcg tcggatcgcc
     9061 cggatgcggc agtatctggt cgacgccgcg accggtcatc acgtcgccgc acaagaagat
     9121 ggtcagaccg tccatctcag cttgagctgt cgtcatcacc aactccgagg ggcctcgact
     9181 gccaccttat tgtcgggctg tccgtcccgg gtacattacg cggggtcgaa ggcgtaggcc
     9241 cggccgtcgc tggtcgcagt gatgacgctg cggtcggtgg ccaccgagac gcccaccggc
     9301 cagccggtgg cctgtggcag gggatacgtg ttgacggtgc gcccgtcgcc gatgcggaag
     9361 accagcagag ccagcgcatt gtcgccggaa cgcaccaccg tgtagccgat gtcgtcgccg
     9421 gcctggctgg acgtgcacag cggggtgacg tcgtcgcgtc gccagatcac gtcgccgcgc
     9481 tcgccgttgt cgcggacggc gaccagcttg gcgccgggcc cgccgccgga cacgagcagg
     9541 ccgccggggg atacagacgg cggtgtttgt ggtgaaaaac ccaagggcac agaccatttc
     9601 ggcttgccgt ctttgccgtc gagcgcccac agcttgccgt cgcggctgtt cacatacacc
     9661 gtcgacccgt cggcggagaa caccggactg cccagtacac cgcccgtgat cgcctcggag
     9721 ttccactccg gcgacagcac ttggcccacg tcggcgcgat acctcagtgc ggtgagggtg
     9781 gccgccgggg ccccgggctg ccagatgccg ctgacgatca ggcccgccga cggcgagagc
     9841 gcgggcgccg ccgcgacggg gcagccgggc ccggcggcct ggcagtcact gagcccgcgc
     9901 tggctgtcgg tggggtcaac gccttgaacg aggtccaccg ccgacccgac catctcgccg
     9961 cggtgcgcgt cgaccacttg gacttggccg agatgggtga ccaccagcag ccggttgggg
    10021 ccgagaaacc gcggggtctg cggcatcccg atcaccggat gccgccagcg cacccactgc
    10081 gtcgtcggaa acgacagcat catgccgggc tgcccgacgt acaggttgtc gaacttgtcg
    10141 aacagcgggc tggcgaaccc gccgccgagc accagccggg tgcaccagcg ctgccggccg
    10201 ttgttgtcgt gctcccacac catcagcgaa caaccggccg cggtctgcgc gttggcggcc
    10261 agatagttgc tggccccgag cgccaccgac gcgaacaggc tgcctttgac cgagcggctc
    10321 cacgtcagct tcagggactc agcgccggcc gtggtggtgt agctgctgtt tgcggcgtcg
    10381 gcgtattggg ccgaccaacc ggccgcgggc gctgcatcca cccaggtgtc ggtgtttccg
    10441 caacccgata ccaggagcaa tgccgcgccc acgagtgccg ccagccgccg gggtgtgcgg
    10501 tgtcgcgaga acatgcgtcc ttccgtcggc gggcgtggcc tcatgagggt aacccggcgg
    10561 ttcctgcagt ccggcgtagg ctttgcggcc atgacgacga tgtggggagc accgttgcac
    10621 aagcgctggc ggggttcgcg gctgcgcgac ccgcgtcaag cgaagtttct gacgctggcc
    10681 tcgctgaaat gggtgctggc caaccgcgcg tacacgccgt ggtacctggt ccgctactgg
    10741 cgactgctga agttcaagct ggccaacccg cacatcatca cccggggcat ggtttttctg
    10801 ggcaaaggtg tcgagattca gtcgaccccg gaactcgcgc agatggacat cggccgctgg
    10861 gtgcacatcg gcgacaagaa cacgatccgc tgccacgagg gttcgctgcg gttcggggac
    10921 aaggtcgtcc tcggccgcga caacgtcatc aatacctacc tcgacatcga gctgggcgac
    10981 tcggtgctga tggctgactg ggtttacgtc tgcgacttcg accatcggat ggacgacatc
    11041 aacatgccga tcaaggacca gggaatcctc aagagcccgg tgcggatcgg gcccgacacc
    11101 tggatcgcgg ccaagtgcac cattttgcgc ggcacgatcg tcggccgcgg ctgtgtgctg
    11161 gggtcgcacg ctgtcgtcaa gggccacatt ccggactact cgatcgcggt cggtgccccg
    11221 gccaaggtgg tgaagaaccg tcgggtggcg tgggagacct cggccgcgga gcgggcacag
    11281 ttggccgccg cgctggccga catcgaacgc aaaaaagcca gccgctaggg tttcccttct
    11341 gcgcgagcgt gcgtgtcgcg cctgcgacac gccgtcgcgt ctggcatttc gcgcacgctc
    11401 gcggttatgg caggaggctg cgcgggtcgt cgggaacctg tggtgtctcc gcgtccgact
    11461 ttgaccgggg cgtcgacttc tccaggaacc gcagcagttc gacggggaac ggcagcacca
    11521 gggtcgagtt cttctcggcg gccacctcga cgaccgtttc gagaagccgt agctggagag
    11581 aagaggggct cgcggccatg actcccgccg cctgagccag cttctgggac gcctgcagtt
    11641 cgccgtcggc cgtgatgacc cgggcacgac gctcgcgttc ggcttcggct tggcgcgcga
    11701 tcgaccgctt catcgaatcg ggcagggcca cgtccttgat ctcgacgcgg tcgatgtgaa
    11761 tgccccagcc catcgcgggg ctgtccagca tcag
//
LOCUS       DASWBF010000440         5899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_437005,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000440 DASWBF010000000
VERSION     DASWBF010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437005"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..649
                     /locus_tag="VF874_16215"
     CDS             113..649
                     /locus_tag="VF874_16215"
                     /inference="COORDINATES: protein motif:HMM:NF022198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HEX9177546.1"
                     /translation="MSAQASGPHQTVGDYLKENNIQETPISRGTPGAPVVDLGLPDGW
                     TAQPESDDAPYGAIVFDTPSDPNDPPRFRAFFEKLTGNVDTDKLLAASPGEVLNLPGY
                     EGGEGQKDTLANFPAYSVGGVYTKDGAKRMAGQKAVVIQGSDGIYLLLLVGDAPGADG
                     SALLDATQAIDERTTITA"
     gene            856..1518
                     /locus_tag="VF874_16220"
     CDS             856..1518
                     /locus_tag="VF874_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HEX9177547.1"
                     /translation="MGVAIAAISLSVGLVGCGKDSKTSTTSTSTTTSPTTTSAAATTS
                     AQASGPAQTLEEYLQANNIQATVVTHGTPGAPTIDLPVPEGWSQLPESEDAPYGGISY
                     DAPSDPASPVAFKARLSKLTGNIDTDKLLVASVGELKNQQGFDGGDGEKSTLSGFPAY
                     QILGSYTKNGAQRVGAEKTAVIQGADGIYLLEIAGSGPEADAQAIQNATNVIDEKTTI
                     TP"
     gene            complement(1508..2077)
                     /locus_tag="VF874_16225"
     CDS             complement(1508..2077)
                     /locus_tag="VF874_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX9177548.1"
                     /translation="MVSERSDSPASTKIVIAGGFGSGKTTFVGAVSEIMPLRTEAILT
                     TASVGVDELEGTPDKKTTTVAMDFGRITLGDEDLVLYLFGTPGQRRFWFMWDDLVRGA
                     IGAIILVDIRRLQDSFAAVDFFEALGLPFLVAVNEFDGAPSYPIDEVRKALSLSAGVP
                     VVSVDARQRDSAKQALIAIAEYALAQLTV"
     gene            complement(2058..2414)
                     /locus_tag="VF874_16230"
     CDS             complement(2058..2414)
                     /locus_tag="VF874_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEX9177549.1"
                     /translation="MDPRSEPNLVRPYTLTAGRTTTNVVLPLEAPIEAFESARAHQWP
                     PNDIRGDIVKLCAARPSVAEIAARLNLPLGVARVLVGDLVETGHVRVLSTLGDSSSVE
                     ERQELIGRTLRGLRTL"
     gene            complement(2421..2837)
                     /locus_tag="VF874_16235"
     CDS             complement(2421..2837)
                     /locus_tag="VF874_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX9177550.1"
                     /translation="MTQPTHPTHGKPLDWLVSAFARDVPGVSHAVLVSADGLLVAASE
                     HLPVERADQLAAVSSGLASLSTGAAQLFEGGGVLQSVVEMENGYLLLMRVGDGSHLAT
                     LASASCDIGQVGYEMAILVERVGGVVKSSRRAAQHS"
     gene            complement(2834..5395)
                     /locus_tag="VF874_16240"
     CDS             complement(2834..5395)
                     /locus_tag="VF874_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9177551.1"
                     /translation="MTMFEQPVLPEETKENPPVAPAKRPSRWSLSNWPVRWKVLAIVI
                     IPIAVAAVFGGWRVYNSVTDARDLRSAAERADMVGPIQDYTGALESALLAYSSGSDGP
                     VARKAFDDSRSELERKLSVTDVDPDVRGGITTLLNGGPPLLDRVASNSIGLRDTVQTY
                     APILLTAEDAIVGSVRMSDEKIRAQAQGLSRAVGTRGQTLMQKLLVTRGGEMPEPELR
                     TSMITLAGTEPSTLSGMNQVLGVGSADAKALQDQMFARTAIISDPAAVLPGNSDLLAS
                     LQATDDIAKKVISDTTSSVTAAVDEQASSARNAAIRDAVIVLAAILAAILVVLLVARS
                     LVRPLRQLREGALRVAHEELPAEVERIQAGAEPGPIQPIPVYTTEEIGQVAHAVDDLH
                     EQALLMAAEQSRLQLHVSDMFETMSRRSRSLVDQQLALIDELERNEDDPQRLDALFKL
                     DHLAARMRRTGTNLLVLSGAKIRREQSESMPVADVIKAAASQVEDYQRVVTATVPDSA
                     IVGSAASDVVHLLAELIDNALRYSPPGSEVRVSAAHTGSRGLVVEVSDAGLGMTEGDL
                     RMTNMRLESGGEVTPYTTRHMGLYVVGRLAAQHGLVVRLRSSMAGQPDSGTTVGVFIP
                     AELLDRPGMLGWRGPEDRPVAATPREFHAPPPMHRNGTPHDEESSPPRRAPGASGIVG
                     VPEEELPPARAADVPSEPAAAQPETEVHEPASRTRPPSSPLGDTDVIFQKMMSEWLID
                     PQDLMQPFQSWESVWDSGWEAAAQAEEVPVDQRTEHGLPVREPGARLVPGSAEPAPTG
                     RPNGAAHRKPDEDNGVADEPVIGRDPEAVRASLSSHWSGIRAGRTHAREDEPENE"
BASE COUNT         1053 a         2017 c         1866 g          963 t
ORIGIN      
        1 tgccatcgcg gccatctcgc tcagcgtcgg actcgttggg tgcggaaagg attccaaaac
       61 gagcaccacc tcgaccacga ccacgaccac gaccacgacg tcgaccacga ccatatccgc
      121 gcaggcgtcg ggacctcatc aaacagtcgg cgactacttg aaagagaaca acatccaaga
      181 gacgccgata agccgcggca cccctggcgc gccggtcgtc gacctagggc tgcctgacgg
      241 atggacggcg cagcccgaga gcgacgacgc gccctacggc gccattgtgt tcgatacccc
      301 gtccgatccc aacgacccgc cccgattccg cgcgttcttc gagaagctga ccggcaatgt
      361 cgacacagac aagctactgg ccgcttcgcc gggcgaggtc ctgaacctgc cgggttatga
      421 aggtggcgaa ggccaaaaag acacactggc taacttcccg gcatattcgg tgggcggcgt
      481 atacaccaag gacggggcga agcggatggc cgggcagaag gccgtggtga tccaaggctc
      541 ggacggcatc tatctgcttc tactcgttgg tgacgcaccc ggcgcggacg gttcggcatt
      601 gttagacgcc acccaagcga tcgacgagag gaccaccatc acagcgtaag ttgcttcgcc
      661 gaatcacgtt gccgggcacc ggcactgatg acctgcgccc ctgtggtcaa gggtgctgcg
      721 atttctcgtg caatgagatt tcgattccgg accgtcgcag acacttcggc atgatttaat
      781 ccggcgagcg ttttgcgaca accaacgcac cacccgagcg caggagagca gcctatgaat
      841 aagtcagcag caatcgtggg cgttgccatc gccgccatct cgctcagcgt cggactcgtc
      901 gggtgcggca aggattccaa aacgagtacg acgtcgacat cgacgacgac ctcaccaacc
      961 acgaccagcg ccgcggcgac gacatccgcg caagcatcgg gtcctgctca gacgttggag
     1021 gaatacctgc aggcgaacaa catccaggcg accgtggtca cacacggcac acccggcgcc
     1081 ccgacgatcg acctgcctgt gcctgagggc tggagccagc ttccggagag cgaggacgcg
     1141 ccctacggcg gcatctccta cgacgcccca tctgacccgg cctccccggt ggcattcaaa
     1201 gcccgcttgt caaagctgac cggcaatatc gacaccgaca agctgctggt cgcgtccgtc
     1261 ggcgagctca agaaccagca gggtttcgac ggtggcgacg gtgaaaagag cacactcagc
     1321 ggctttcccg cctaccagat tctgggctcg tacaccaaga acggagcgca acgggtaggc
     1381 gccgaaaaga ccgcggtgat ccagggcgcg gacggcatct acctgctcga gatcgccgga
     1441 agcggacccg aggccgacgc tcaggcgatc cagaacgcga cgaacgtgat cgacgaaaag
     1501 accacgatca caccgtaagt tgcgccagcg cgtactccgc tatcgcgatc agtgcctgct
     1561 tcgccgaatc acgttgccgg gcatcaacac tgaccaccgg cacccctgcc gatagcgaaa
     1621 gtgctttgcg cacttcgtcg atcgggtaac tcggcgcgcc gtcgaactca ttgaccgcaa
     1681 ccaagaacgg cagcccgagg gcttcgaaga agtcgacggc ggcgaaactg tcctgcagtc
     1741 gccggatgtc cactaggatg atcgcgccga ttgcgccgcg caccaagtca tcccacatga
     1801 accagaaccg ccgctgcccc ggggtgccga acaagtagag gaccagatcc tcgtccccca
     1861 gtgtgatccg gccgaagtcc atggccaccg tggtggtctt cttgtccggt gtgccctcga
     1921 gttcgtcgac acccacggac gccgtcgtga ggatggcctc agtgcgcaac ggcatgatct
     1981 cggacacggc gccgacgaaa gtggtcttgc ccgagccgaa tccgccggcg atcacgatct
     2041 tggtggacgc aggagaatca gagcgttcgg agaccacgca gcgtccttcc gatcagctcc
     2101 tgtcgctcct ccaccgacga gctgtcgccg agagtgctca gcacacgcac atgcccagtc
     2161 tccaccagat cgccaaccaa cacgcgcgcc acgccgaggg gcaggttgag ccgcgccgct
     2221 atctccgcga cggagggccg ggcggcacac agcttcacga tgtcccctcg tatgtcgttg
     2281 ggtggccatt ggtgagcgcg ggcggactcg aacgcctcga tcggcgcctc caacggcaac
     2341 acaacgttcg tggtcgtccg cccggccgtc agagtgtacg gccgcaccaa gttcggttcg
     2401 ctgcgggggt ccatcacgcc tcaagagtgc tgggcagctc gtcgcgacga cttcacaacg
     2461 ccgccaacac gttccaccaa gatcgccatc tcgtaaccca cctggccgat gtcacaggag
     2521 gcggacgcca acgtcgcgag gtgcgacccg tcgcccaccc gcatcagcag cagatacccg
     2581 ttctccatct cgaccaccga ctgcagcacc ccgccacctt cgaacagctg cgccgccccg
     2641 gtggacaagc tcgccaatcc cgaactcact gcggctaatt ggtccgcccg ttcgaccgga
     2701 agatgctcgc tggccgccac cagcagcccg tcggccgaca ccagcactgc atgcgaaaca
     2761 ccgggcacgt cgcgggcgaa cgcggagacc agccagtcca gcggcttgcc gtgagttgga
     2821 tgtgtcggct gcgtcattcg ttctccggct cgtcctctcg ggcgtgggtg cgtcctgcgc
     2881 gaatcccgct ccagtggctg gacagcgacg cgcgcacggc ctccggatcg cgtccgatga
     2941 cgggctcgtc ggcaacgccg ttgtcctcgt cgggtttccg gtgtgcggcg ccgttgggcc
     3001 ggcccgtcgg ggcgggctcg gccgatccgg gcaccaggcg ggcgccgggc tctcgcaccg
     3061 gcaaaccatg ctccgtgcgc tgatcgaccg gcacctcctc ggcctgcgcg gcggcctccc
     3121 agccgctgtc ccacacggac tcccacgact ggaacggctg catcaagtcc tgcgggtcga
     3181 tcagccactc cgacatcatc ttctgaaaga tcacgtcggt gtcgccgagc ggtgacgacg
     3241 gcggccgggt gcgcgacgcg ggttcgtgca cctcggtttc cggctgtgca gcggccggct
     3301 ctgacgggac gtctgcggcg cgcgcggggg gcaattcctc ctcgggcaca cccacgatcc
     3361 cgctggcccc gggtgctcgg cgaggcggcg acgactcctc gtcgtgcggc gtgccgttgc
     3421 ggtgcatcgg cggcggggcg tggaactcac gcggcgtggc agcgaccggg cggtcctcgg
     3481 gcccccgcca gccgagcatt ccgggtcggt cgagcagttc ggctggaatg aagacaccca
     3541 cggtcgtgcc ggagtccggt tgccccgcca tcgagctgcg cagccgcacc accaggccgt
     3601 gctgggcagc gagccgtcca accacgtaca gacccatgtg gcgggtggtg tagggggtga
     3661 cctcgcctcc ggactccaga cgcatgttgg tcatccgcag gtcgccctcg gtcatgccga
     3721 gcccggcgtc gctgacctcg acgaccaggc cacggctgcc ggtatgcgcc gcggacaccc
     3781 gcacctccga ccccggcggc gaatagcgca gcgcgttgtc gatcaactcg gcgagcaggt
     3841 gcacgacgtc gctggccgcc gaaccgacga tcgcgctgtc cggcaccgtc gccgtcacga
     3901 cgcgctgata gtcctccacc tgcgacgcgg ccgccttgat gacgtcggcg accggcatgg
     3961 actcggactg ctcgcgacgg atcttcgcgc ccgacagcac cagaagattg gtgccggtgc
     4021 ggcgcatccg cgcggcgagg tggtcgagct tgaacagcgc gtcgaggcgc tgcggatcgt
     4081 cctcgttgcg ttcgagttcg tcgatcaacg ccagctgctg gtcgaccagc gatcggctgc
     4141 gccgcgacat cgtctcgaac atgtcgctga cgtgcagttg cagccgcgac tgctcggccg
     4201 ccatcaacag cgcctgctca tggaggtcgt cgacggcgtg ggcgacctgg ccgatctcct
     4261 cggtggtgta caccgggatc ggctggatcg gcccgggctc ggcaccggcc tggatgcgtt
     4321 cgacctcggc aggcagttcc tcgtgcgcca cccgaagcgc gccttcgcgc agctggcgca
     4381 gcggacgcac cagcgaccgg gcgaccagca gaacaaccag aattgcagcc aagatcgcgg
     4441 ccagcacgat caccgcgtcg cgtattgcgg cgttgcgcgc cgacgacgcc tgctcgtcga
     4501 ccgcggcggt gaccgacgac gtcgtgtcac tgatcacctt cttggcgatg tcgtcggtgg
     4561 cctgcagaga cgcaagcagg tccgagttgc cggggagcac ggcggccggg tcggagatga
     4621 tggccgtgcg ggcgaacatc tggtcctgca gcgccttcgc gtccgctgag ccgaccccga
     4681 gcacctggtt cattccggac agcgtggacg gctcggtacc ggcgagcgtg atcatcgacg
     4741 tgcgaagttc cggctccggc atctcgccgc cgcgagtgac cagcagtttc tgcatcagcg
     4801 tctggccgcg ggtgcccacg gcgcggctga ggccctgggc ctgggcgcgg atcttctcgt
     4861 cgctcattcg caccgacccg acgatcgcgt cctcggcggt gagcaggatc ggcgcgtacg
     4921 tctgcacggt gtcgcgcaac ccgatgctgt tggacgcgac ccggtccagc agcggcggac
     4981 cgccgttgag cagggtggtg atgcctccgc gcacgtcagg gtcgacgtcg gtgaccgaca
     5041 gtttgcgctc taactcggag cgggagtcgt cgaacgcttt gcgcgccacc gggccgtcgg
     5101 aacccgagga atacgccagc agagcgcttt ccaacgcgcc ggtgtagtcc tggatcggcc
     5161 cgaccatgtc ggcgcgctcg gcggcgctgc gcagatcgcg cgcgtcggtg acgctgttgt
     5221 agacgcgcca accgccgaac acggccgcga ccgcaatcgg aatgatgacg attgcgagca
     5281 ccttccagcg caccggccaa ttcgacagcg accatcgtga cgggcgcttg gccggcgcga
     5341 caggcggatt ctctttcgtc tcctcaggca gcaccggctg ctcgaacatg gtcatcgttg
     5401 cacgctcttg tgaacgaccg attcgatgtg ctgaatgtgc gcctggttca tagactttcc
     5461 tgcttttcga cccgacgtcg ggcggcgcgg taaccgcctg gcaatccgac gagtatgcca
     5521 gccgccgcct gtagccacca ccggttctta ctgaacagag agagttcaca ggcgctaccc
     5581 cgcgccgcgg aaattctacg tgagcaaatt ttcgtccgcg aagggcggct ttattgttgt
     5641 tgccgttctg gaagtttagt ttgctcttcg cgcaagtgcc catcgcgcgc gatcatttgc
     5701 acgtgttccg cgtgatgttt cactcaccgc gcatcgatgt gcccacaaca ctcacgacgg
     5761 gcgtacaccg cgctacggcg gcgtagacag cctgcaaagc gtggcctgaa cattggcgaa
     5821 atatcgggac tcgtagccat gaccagtaca aaattccgcc atgagcgact atctgctagt
     5881 ccatggcggg ttcaacggc
//
LOCUS       DASWBF010000441         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_437271,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000441 DASWBF010000000
VERSION     DASWBF010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437271"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..545)
                     /locus_tag="VF874_16245"
     CDS             complement(78..545)
                     /locus_tag="VF874_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX9177552.1"
                     /translation="MTYDPNDPNRPFEYPSLEGYPSPPANPYPPVDYPSTYPPGAPPG
                     YPPYPPPYQGYGANPYNPYDPYAAPPSQGTSGMAIGALVTSLLGLPLCFCFIPSLVGI
                     VLGVVAMTETKRTGKEGHGMALAAVIVGAITLVLGIGFWLITALAPDTETSSY"
     gene            complement(559..1074)
                     /locus_tag="VF874_16250"
     CDS             complement(559..1074)
                     /locus_tag="VF874_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX9177553.1"
                     /translation="MSTPGGPGERWWENQPPSGGYESPPSYPAPPSSYPAPPSGYPPP
                     PSSYPPPSAYPPPPSGYPPSAPSGYPPSAPYQPGYPIAPQSHTNGLAIGSLVASILAI
                     PLALFCFGFGGAVAAIAGIVMGVVALNQIKQSGDQGRGLALSGVIIGGVALVLGLIFI
                     ILLAIGASYQS"
     gene            1219..1731
                     /locus_tag="VF874_16255"
     CDS             1219..1731
                     /locus_tag="VF874_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX9177554.1"
                     /translation="MTSPFPQGQNPNTPAPGARGPSLPTPPKGWPIGSYPAYAEAQRA
                     VDYLADQQFPVQQVTIVGVDLMQVERVTGRLTWPKVLGGGVITGAWLGIFIGLVLGVI
                     TSNPWAPLLTGVIAGIFFGLITSAVPYAMTRGTRDFSSTMQLVAGRYDVLCDPQNAEK
                     ARDLLARLTL"
     gene            1888..3309
                     /locus_tag="VF874_16260"
     CDS             1888..3309
                     /locus_tag="VF874_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9177555.1"
                     /translation="MRVPGKSRSSLGRLGAAAVAALTAASVVSACGSGSNGIVISFYT
                     PAGDANTFAAIAKKCSEQFDGRFTIQQMSLPKGADDQRLQLARRLTGNDRTLDVMALD
                     VVWTAEFAEAGWALPLSDDPAGRAEADAVTDTLPGPLKTAEWNDKLYAAPISTNTQLL
                     WYRPDLVTTPPTDWDGMVAEATSLYNAGKPSWIAVQGKQYEGLVVWFNTLLESAGGQV
                     LSDDGQTVTLTDTPQHREATVRALQIIKNVATAPGADPSITQTNEETARLALEQGKAA
                     LEVNWPFVLPSMMGNAIKGGVSFLPLNQRSDLAGAINDVGDFDADDAQFAAAYDATKA
                     VFGFAAYPGVQPGVPARVTLGGLNLAVAKTTGHKAEAFEAVRCLRSLENEKYLSIQGG
                     LPTVRASLYDDPQFQLKYPAYEIIRNQLTDAAVRPLTPNYQSVSMRISATLAPITEID
                     PEKTADKLAAEVQKAIDGKGLIP"
     gene            3306..4229
                     /locus_tag="VF874_16265"
     CDS             3306..4229
                     /locus_tag="VF874_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9177556.1"
                     /translation="MTRTVALRASGSSAASDERRSQRRLAYLLITPAVLLMLAVTAYP
                     IAYAVWLSLQKVSLATPGDSTFVGLANYQTVLTDRYWWTAFAVTLAITVVSVSIELVL
                     GLALALVMHRTIFGKGVVRTAVLIPYGIVTVAASYSWYYAWTPGTGYLANLLPDGSAP
                     LTHQIPSLGIIVLAEVWKTTPFMALLLLAGLALVPDELLRAAQVDGAGAWKRLVKVTL
                     PLIKPAILVALLFRTLDAFRIFDNIYVLTGGANNTGSVSILGYDNLFKAFNLGLGSAI
                     SVLIFLCVAVIAFIFIKLFGTSAPGADDGNR"
BASE COUNT          741 a         1380 c         1469 g          708 t
ORIGIN      
        1 aagatggcgc tggtgatcgc cgccaagggc agggccgcgg gcatgacgcg gcaaggcgag
       61 cggttccagc agccgagcta gtagctgctg gtctcggtgt cgggggccag ggcggtgata
      121 agccagaacc cgatgccgag caccagcgtg attgctccga cgatgacagc ggcgagagcc
      181 atcccatgtc cctctttgcc ggtgcgcttc gtttcggtca tcgcgacgac gcccagcacg
      241 atcccgacaa gcgacggaat gaagcagaag cacagcggca gccccagcaa cgacgtcacc
      301 agcgcgccga tcgccatgcc gctggtgcct tgcgacgggg gcgctgcgta cgggtcgtac
      361 gggttgtacg gattggcgcc gtacccctga tacggaggcg ggtacggggg atagccgggg
      421 ggcgcgccgg gcggatacgt cgacgggtag tcgaccggcg ggtacggatt ggccggagga
      481 cttggatacc cctccaacga cgggtattcg aagggccggt tcgggtcgtt cgggtcgtaa
      541 gtcacggcac cgtcatgact agctttggta gctggcacct atagccagca ggatgatgaa
      601 gatcagtccg agcacaagcg ccacgcctcc gatgatgacg ccggacaaag cgagtcctcg
      661 cccctggtct ccactctgct tgatttggtt cagcgcgacg acgcccatca cgatgccagc
      721 gatggcggcc actgcgccgc cgaatccaaa gcagaagagt gccagtggaa tggcgaggat
      781 cgaggccacc agcgagccga tcgccagtcc gttggtgtgt gactgcggcg cgatcggata
      841 gccgggctga tacggagccg acgggggata accgcttggt gccgacgggg gataaccgct
      901 tggtggaggt ggatatgccg acgggggcgg atagctcgaa ggcgggggcg gatagcccga
      961 aggcggagcc ggatagctcg aaggcggagc cggatagctc ggcggtgact catatccccc
     1021 cgaaggcggt tggttctccc accaccgctc gcctgggccg cccggtgtgc tcatggcgat
     1081 caacctagcg tattagcagg tcgggcgggc cgctgtcggc acgatgacgg gcaaatcggg
     1141 gcacaatcaa gcggtgaggc gcgttgggtg tgcagactgt gcccggcccg gcgcacacat
     1201 cgtgaggagt aaggacggat gacgagccca tttccgcagg gacagaaccc caataccccg
     1261 gcgccgggcg ctcgcggccc gtcgctgccc accccgccga agggctggcc gatcggctcg
     1321 tacccggcgt acgcggaggc gcaacgtgcc gtcgactacc tcgccgacca gcagttcccg
     1381 gtccagcagg tcaccatcgt cggcgtcgat ctgatgcagg tcgaacgggt caccggccgg
     1441 ctcacgtggc cgaaggtcct cggcggcggc gtgatcaccg gcgcgtggct gggcatcttc
     1501 atcgggctgg tgctcggggt gattaccagc aacccgtggg caccgctgtt gacaggcgtg
     1561 atcgccggca tcttcttcgg gttgatcacc tctgcggttc cgtacgcgat gacgcgtggc
     1621 acaagagatt tcagctcgac gatgcagctg gtggccggcc gctacgacgt gctgtgcgat
     1681 ccgcagaacg ccgaaaaggc gcgagacttg ctggcccgct tgacgttata gcggctgcac
     1741 cagtcgttgg ctctgcgctg agggcgttgc tttctcgcac ttccgcgccc tggttgcaga
     1801 gtcgatgcgc attgtgccgc gggccggtaa cgattggcga cgcgggggct ccacgtgttg
     1861 cacttcacgc gcatctgcct ctaccgtttg cgcgtgcccg gcaagtcgcg cagctcactc
     1921 ggccggctcg gtgcggcggc cgttgcggcg ctgaccgcag cgtcggtggt ctcggcctgc
     1981 ggatccggca gcaacggcat cgtgatcagc ttctacaccc ccgcgggcga tgcgaacacg
     2041 ttcgctgcga tcgccaaaaa gtgcagcgag cagtttgacg ggcggttcac gatccagcag
     2101 atgagcctgc cgaaaggcgc cgacgaccag cggctgcagc tcgctcgtcg gctcaccggt
     2161 aacgaccgga ccctcgacgt catggcgctc gacgtggtgt ggaccgccga gttcgccgag
     2221 gctggctggg cattgccgct gtccgacgat cccgcgggac gggccgaggc cgacgccgtc
     2281 accgacactc tgcccgggcc gctgaagacc gccgagtgga acgacaagct ctacgcggca
     2341 ccgatcagta ccaacaccca attgctttgg taccgacctg atttggtcac cacaccgcca
     2401 accgactggg acggcatggt ggccgaggcc accagccttt acaacgcggg caagccgagc
     2461 tggatcgcgg tgcagggcaa gcagtacgaa ggcctggtgg tctggttcaa cacgttgctg
     2521 gaaagcgcag ggggacaggt gctttccgac gacggccaga ccgtcacgct cacggacacc
     2581 ccgcagcacc gggaagccac ggtcagggcg ctgcagatca tcaagaacgt ggccaccgcc
     2641 ccgggtgccg acccgtcgat cacccagacc aacgaggaga ccgcgcggct ggcgctggag
     2701 cagggcaagg cggcgctgga ggtgaactgg ccgttcgtgc tgccgtcgat gatgggcaac
     2761 gcgatcaaag gcggggtgtc gttcctgccg ctcaaccagc gaagcgacct ggccggcgcc
     2821 atcaacgacg tcggggactt cgacgccgac gacgcccagt tcgccgccgc ctacgacgcc
     2881 accaaggcgg tgttcggctt cgcggcgtat ccgggcgtgc aaccgggcgt gccggccagg
     2941 gtcaccttgg gcgggctgaa cctggcggtg gcgaagacca ccgggcacaa ggccgaagcg
     3001 ttcgaggccg tccgctgcct gcgcagtctg gagaacgaga agtacctgtc catccagggc
     3061 ggtctgccca cggtccgggc gtcgctgtac gacgacccgc agttccagct caagtacccg
     3121 gcgtacgaga tcatccgcaa ccagctcacc gacgccgcgg tgcgccctct gacaccgaac
     3181 taccagtccg tgtcgatgcg gatctcggcc acgctcgctc cgatcaccga gatcgacccg
     3241 gagaagaccg ccgacaaact cgccgccgag gtgcagaagg cgatcgacgg caaggggctg
     3301 atcccgtgac gagaaccgtc gctcttcgcg caagcggctc atcggcggcg tccgacgagc
     3361 gcaggtcaca gcgacggctg gcgtacctgc tcatcactcc cgcggtgctg ctcatgctgg
     3421 ccgtcaccgc ctacccgatc gcctacgcgg tgtggctcag tctgcagaag gtcagcctgg
     3481 ccactcccgg cgactccacg ttcgtcgggc tggcgaacta tcagaccgtg ctcaccgacc
     3541 gctactggtg gacggcgttc gcggtcaccc tggcgatcac ggtggtctcg gtgtccatcg
     3601 agttggtgct cggcctggcg ctggcgctgg tgatgcaccg gaccatcttc ggcaagggcg
     3661 tggtgcgcac cgcggtgctg atcccgtacg gcatcgtgac cgttgcggcg tcctacagct
     3721 ggtactacgc gtggacaccg ggcaccggct atctggccaa cctgctgccg gacggcagcg
     3781 cgccgctgac acatcagatc ccgtcgctgg gcatcatcgt gctcgccgag gtgtggaaga
     3841 ccaccccgtt catggcgctg ctattgctgg ccggtctggc gctggtaccc gacgagttat
     3901 tgcgcgctgc gcaagtggac ggcgcgggag cgtggaagag gctagtcaag gtgacgctgc
     3961 cgctgatcaa gcccgcgatt ttggtggcgc tgctgttccg caccctggat gcgttccgca
     4021 tcttcgacaa catctacgtc ctcaccggcg gtgccaacaa caccggctcg gtgtcaatcc
     4081 tgggctacga caacctgttc aaggccttca acctcggtct gggctcggcg atcagcgtgc
     4141 tgatcttcct gtgcgtcgcg gtgatcgcat tcatcttcat caagctgttc gggacgtcgg
     4201 cgcccggagc ggacgacggg aatcgctgag atggcagaaa gagtcggcgc cggccgaaaa
     4261 gcgtggtggg tgatcgtcga cgtcctcgtg gtcgtcta
//
LOCUS       DASWBF010000442         4627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_437324,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000442 DASWBF010000000
VERSION     DASWBF010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437324"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VF874_16270"
     CDS             <1..546
                     /locus_tag="VF874_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX9177557.1"
                     /translation="ATARRLGARTSGYDVRPEVAEQVRSVGAQWLDLGIDAAGEGGYA
                     RELTDQERAQQQKKLEEAIKGFDVVITTALVPGRPAPRLVTAEAVAGMAPGSVVVDLA
                     GEAGGNCELTEPGRTVVKHDVTIASPLNLPATMPEHASELYSKNITALLELLVKDGRW
                     APDFDDEIVAEACVTRTGGDS"
     gene            547..876
                     /locus_tag="VF874_16275"
     CDS             547..876
                     /locus_tag="VF874_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX9177558.1"
                     /translation="MYEQLLANLAILVLSGLVGFAVISKVPNTLHTPLMSGTNAIHGI
                     VLIGALVVFGRVEHPSIAIQAILFVAVVFGTLNVVGGFVVTDRMLGMFKGKKPQAVKA
                     DTDGQVR"
     gene            873..2294
                     /locus_tag="VF874_16280"
     CDS             873..2294
                     /locus_tag="VF874_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX9177559.1"
                     /translation="MNYLVVLLYLVSFAMFIYGLMGLTGPKTAVRGNLIAAAGMAIAV
                     VATLIAIRDTDHWPLIVAGLLLGVVLGVPPARLTKMTAMPQLVALFNGVGGGTVALIA
                     LAEFIDTEGFSAFKHGESPTAHIVVASLFSAIIGSISFWGSLVAFGKLQEILPQRPIG
                     LGKAQQALNALLLLGSIACAVVIGLGAHPGEGVSLWWMIGVLAAAAVLGLMVVLPIGG
                     ADMPVVISLLNAMTGVSAAAAGLALNNTAMIVAGMIVGASGSILTNLMAKAMNRSIPA
                     IVAGGFGGGGVAPTGGDGVDRTVKSTSAADAAIQMAYANQVIVVPGYGMAVAQAQHAV
                     KDMAALLEKRGVPVKYAIHPVAGRMPGHMNVLLAEADVEYDAMKQMDDINDEFARTDV
                     AIVIGANDVTNPAARSDASSPIYGMPILNVDKSKSVIVLKRSMRSGFAGIDNPLFYGD
                     GTTMLFGDAKKSVSEVTEELKAL"
     gene            complement(2800..4329)
                     /locus_tag="VF874_16285"
     CDS             complement(2800..4329)
                     /locus_tag="VF874_16285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX9177560.1"
                     /translation="MRSNLLDGREAVFAALEALEADADVFAGLSFEALSEMDCVLVMS
                     RLMTIARKVPAWHYELVNQLGERAVPSDIGGPLPRVLADRLKIRPSAARRLISDAGQL
                     GHRRALSGEPLEPLWPTTASKARAGEIGAEHVAIIRNFFHQLPASYPAQERDRDEQML
                     GEMAAQLRPDQLQQAADRMAALINPDGTFSDLDRARKRGFSWGAQGCDGMSQGTLIAD
                     PQLRATLDAVIAKLGAPGMCNPEDPTPTVDGAPPEESVRADTRTRAQRAHDALTAMGR
                     SVLASGELGQHRGLPATIIVRTTLQELQSGVGKADTGGGSWLPMRDVIKEAKSAYHYL
                     AVFDEHSNRPLYLGRTRIASADQRIVLYARDGGCTAPGCDKPGYECEVMHLRAHARDG
                     LTEPDNLAFGCHTDHTLHDEGWTTRLNPDGIVEWLPTPAPEPQTRHQQLLPPRPIPTR
                     RRRRRPVVGVCSQCQRASWANVVVAETICGAVIAVVPSPRAMPAGGEVLLDDAVICYA
                     A"
     gene            complement(4443..>4627)
                     /locus_tag="VF874_16290"
     CDS             complement(4443..>4627)
                     /locus_tag="VF874_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX9177561.1"
                     /translation="HSESTVMVGDRMDTDVVAGIEAGLETILVLSGSTSLDEVEKYPF
                     RPSLILQSIADAIELV"
BASE COUNT          790 a         1493 c         1559 g          785 t
ORIGIN      
        1 gccacggcca ggcgactcgg cgcccgcacc agcggctacg acgtgcgtcc cgaggtcgcc
       61 gaacaggtgc ggtcggtggg cgcgcagtgg ctggatttgg gcatcgacgc cgccggcgag
      121 ggcggctacg cgcgtgagct gactgaccag gaacgtgcgc agcagcagaa gaagctggaa
      181 gaggcgatca agggattcga cgtggtgatc accaccgcgc tggtgcccgg gcggccggct
      241 ccgcggctgg ttacggccga ggcagtggcc ggcatggcgc ccggcagcgt ggtggtggac
      301 ctggccgggg aggccggcgg caactgcgag ctgaccgagc cgggacgcac cgtcgtcaag
      361 cacgacgtga cgatcgcatc gccgctgaac ctgccggcga ccatgcccga gcacgccagc
      421 gagttgtact ccaagaacat caccgcgctg ctggaactgc tcgtcaagga cgggaggtgg
      481 gcgccggact tcgacgacga gatcgtcgcg gaggcatgcg tcacgcgaac aggaggcgac
      541 agctaagtgt atgaacaatt gttggccaac ctggcgatcc tggtgctgtc cgggttagtc
      601 ggcttcgcgg tgatctccaa ggtgcccaac accttgcaca caccgctgat gtcgggcacc
      661 aacgcgatcc acggcatcgt gctgatcggc gcgttggtgg tgttcggccg agtcgagcac
      721 ccgtcgatcg cgatccaggc catcctcttc gtggcggtgg tattcggcac gctcaacgtc
      781 gtcggcgggt tcgtcgtcac cgaccgcatg ctgggcatgt tcaaaggcaa gaaaccacaa
      841 gcggttaaag ccgacacgga cggacaagtg cgatgaacta cctcgtcgtg ctgttgtacc
      901 tcgtctcgtt cgcaatgttc atctacggcc tgatggggtt gaccggaccc aagaccgcgg
      961 tgcgtggcaa cttgatcgct gccgcgggca tggcaattgc ggtcgtcgcc acgctgatcg
     1021 caatccgcga caccgatcac tggccgctga tcgtcgcagg attgctgcta ggtgtggtgc
     1081 tgggtgtgcc gcctgcccgg ctgaccaaga tgaccgcgat gccccagctg gtggcgctgt
     1141 tcaacggcgt tggcggcggt acggtcgcgc tgatcgcact cgcggaattc attgatacag
     1201 aaggtttttc ggcgttcaag cacggtgagt caccgaccgc gcacatcgtc gtcgcgtcgc
     1261 tgttctccgc gatcatcggg tcgatctcgt tctggggctc gctcgtcgcg ttcggcaagc
     1321 tgcaggagat cctgccccag cggccgatcg gtctgggcaa ggcacagcag gcgctcaacg
     1381 cgctgctgct gctcggctcg atcgcctgcg cggtggtgat cgggctcggc gctcacccgg
     1441 gcgagggtgt ctcgctgtgg tggatgatcg gcgtgctggc cgccgcggcg gtgctcgggc
     1501 tgatggtggt gctgccgatc ggcggcgccg acatgccggt ggtgatctcg ctgctgaacg
     1561 cgatgaccgg ggtgtcggct gcggcagcgg gtttggcgct gaacaacacc gcgatgatcg
     1621 tggccggcat gatcgtcggc gcatccggtt cgatcctgac gaacctgatg gccaaggcga
     1681 tgaaccgatc gatcccggcg atcgtcgccg gcggcttcgg cggcggcggg gtggctccca
     1741 cgggtggcga cggcgtcgac cgcacggtca agtccacctc ggccgccgac gccgcgatcc
     1801 agatggccta cgccaaccag gtgatcgtcg tgcccggtta cggcatggcc gtggcccagg
     1861 cgcaacacgc ggtcaaggac atggccgcgc tcctcgagaa gcgtggcgtg ccggtcaagt
     1921 acgcgatcca cccggtggcc ggccggatgc cgggccacat gaacgtgctg ctggccgagg
     1981 ccgacgtcga atacgacgcg atgaaacaga tggacgacat caacgacgag ttcgcccgca
     2041 ccgatgtcgc gatcgtcatc ggcgccaacg acgtcaccaa cccggcggcg cgcagcgacg
     2101 ccagctcgcc gatctacggc atgccgatcc tcaacgtcga caagagcaag tccgtcatcg
     2161 tgctcaagcg gtcgatgagg tccggcttcg ccggaatcga caacccgctg ttctacggcg
     2221 acggcacgac gatgctgttc ggcgacgcga agaagtcggt cagcgaggtc accgaggagc
     2281 tcaaggcgct gtagggccgg ccattctcgc ggggacggcc aacccgccag tgggaatgag
     2341 gatgtacgtg gctgtggtcc ggaccagcac acggtcttca gggtcgacga ccgaacattc
     2401 ggtgctgagg agcctgcggc ctccgtgcac caatttggct tccgcgatca gtggaccgcc
     2461 atggggctgc cggtaaccgc ggacgtgcat gtccgttgtg acggtgaact gggaactcag
     2521 gtcgtagcaa ccgataaggc aacaggcaca ggccgcatcc gcgaacgttg cgagcatccc
     2581 tccatggacg gtgccttcga aaagcactct gacatcgtcg gtgagcggca tggagagtac
     2641 cgcgagaccc ggcctcctgg cggacgacgc tgaactgcaa tcggtcgtgg acaggagagt
     2701 tgcgtagctc cgcagtgctc aacctgcccc gtcctcctgc ttgctagacc tttcctgccg
     2761 accgagcgga cattcaatgt caatcgacat gaaaatttgt catgcggcat aacaaataac
     2821 agcatcgtca agcaaaacct ccccgccggc gggcatggcg cggggtgatg gaacgacggc
     2881 gataaccgct ccgcaaatag tctcggcgac aacaacattc gcccaagacg cacgctgaca
     2941 ctgcgagcag acaccgacta cgggtcgtct tcgtcgtctt cgggtaggta tcggccgggg
     3001 tggaagaagt tgttgatgcc gggtttgagg ttcaggtgcg ggggtgggga gccattcgac
     3061 gatgccgtcg gggttgagcc gggtggtcca gccttcgtcg tgcagtgtgt ggtcggtgtg
     3121 acacccgaag gccaggttgt cgggttcggt gagcccgtcg cgggcgtgcg cgcgcaggtg
     3181 catgacttcg cactcatagc cgggtttgtc gcagcccggg gcggtgcacc cgccatctct
     3241 cgcgtacagc acgatgcgct ggtccgccga ggcgatgcgg gtgcggccca gatacagcgg
     3301 ccgattggag tgctcgtcga acaccgccag atagtggtag gcggatttgg cttccttgat
     3361 cacgtcgcgc atcggcagcc agctgccgcc gccggtgtcg gccttgccca cccctgactg
     3421 cagttcttgc agggtggtgc gcacgatgat ggtggccggc agcccgcggt gctggcccag
     3481 ctcgccggag gccagcacac tgcggcccat cgcggtcaac gcgtcatggg cgcgctgcgc
     3541 gcgggtgcgg gtatcggcgc gcaccgattc ttcgggcggc gccccgtcca ccgtcggcgt
     3601 cgggtcctcg ggattgcaca tgcccggggc gcccagtttg gcgatcaccg cgtccagggt
     3661 ggcgcgcagc tgcgggtcgg cgatcagggt gccctgactc atcccgtcgc atccttgggc
     3721 gccccaggaa aacccgcgct tgcgggcccg atccagatca gagaaggtgc cgtcggggtt
     3781 gatcagcgcg gccatccggt cggcggcctg ctgcagctgg tcggggcgca gctgcgcggc
     3841 catctcaccc agcatctgct cgtcgcggtc gcgttcctgg gccggatagc tggcgggcag
     3901 ctgatggaag aagttccgga tgatcgccac atgctcggcg ccgatctccc cggcccgcgc
     3961 cttgctcgcg gtggtgggcc acagcggctc cagcggctcc cccgacagcg cccggcgatg
     4021 ccccagctgt ccggcgtcgc tgatcagccg acgcgccgcg ctcgggcgga tcttgagccg
     4081 atcggccagc acccgcggca acggaccccc gatatccgac ggcaccgccc gctcgcccag
     4141 ctgattgacc aactcgtagt gccacgctgg aaccttgcgc gcgatcgtca tcaggcggct
     4201 catcaccagc acgcaatcca tttccgacaa cgcctcaaac gacagcccgg cgaacacgtc
     4261 cgcgtcggcc tccaacgcct caagggcggc gaagaccgcc tcccgaccgt ccaacagatt
     4321 cgaacgcatg agcgaattct atccccgccc gccgacaact tcccgcacca tccacacccg
     4381 aaagacctgt ggacaaaacc ggattcgctc acactgacat gtcagagccc tgtgttagat
     4441 gctcagacca gttcgatcgc gtcggcgatc gactgcagga tcagagaggg ccgaaacggg
     4501 tatttctcca cttcgtccaa cgacgtcgag ccgctgagca ccaggatcgt ttccaggccg
     4561 gcctcgatgc cggcgacgac gtcggtgtcc atccgatcgc ccaccatcac ggtgctctcc
     4621 gagtgcg
//
LOCUS       DASWBF010000443         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_437355,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000443 DASWBF010000000
VERSION     DASWBF010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437355"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VF874_16295"
     CDS             complement(<1..380)
                     /locus_tag="VF874_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease"
                     /protein_id="HEX9177562.1"
                     /translation="MMRQLVSIAAAIALLVLCSPPPQAWALSMPVVDPAALPADGPPG
                     PDQEMRQSGQCSFFGALPGFDPAAVPPSQDMLNLPEAWKTSRGRGVAVAVIDSGVTPQ
                     PRLANLNPGGDYIDPAADGLVDCDG"
     gene            complement(380..1792)
                     /gene="eccD"
                     /locus_tag="VF874_16300"
     CDS             complement(380..1792)
                     /gene="eccD"
                     /locus_tag="VF874_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HEX9177563.1"
                     /translation="MDPAATGQIRVAVIGDGAAADLALPTTLAIRELIPRIRATLASG
                     CDDDELSPEDASEDGLRPYSLAPLGGTPFSLDATLETLAIDDGEQLVLCKLPPGPAAP
                     PVVEDIADASAIHSARQFKPFEHAMLGAMAQAVVLSLGALVCALAVSGWHHGYNLWAA
                     AALGVLGVVFIGATVVLRRRGLTQAAGRMGVATTVPLALALAASLPGEGAAPRVFLAA
                     AGLVTWSLLLLAITNSWVATHTAIIAVSATVAAAAAVRILWHLPYLTLGCGVLAVSLL
                     VASNAPTASAVWARFPLPNVPAPGEPTPAPSSLAEIEDLPRKTATCNSYQSGMVAASV
                     VLSVIGSVLVLWLPAAPSLLAWWLVVTTTTVTVLRMRIWDSAIPSLWFLATPFLSAAA
                     LTVSFTATDHLIAGCYAAAAGVGLTAVLIGAAVIKPRELSIPRRRWLDLFENMLLITI
                     LPAMLWLVGLVSLIRNRGAI"
     gene            complement(1796..3313)
                     /locus_tag="VF874_16305"
     CDS             complement(1796..3313)
                     /locus_tag="VF874_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9177564.1"
                     /translation="MSTPSDFFAPDHEPRPEQSPEQQRRTPAPQENQRPPSSPTAADT
                     GPQRRLPQPQHPADERRSATAPPAGPAPQHAGRPPRPHQPYQPSSQPHPNAAAPNAAT
                     GWQPSSQEPDDNTAMLSQPDAASGWDPSVGGFFTDPPAAQEHSPQQGRPASHPPRNPQ
                     RPVSSPQALFGGPLGDGAAPFGPGGGYGPGTEHERVVSSRRRPAGKGWRKVVSKMTFG
                     LITPGPSAAQVQAEELIRRIRSSLVDVYVVAFVNAKGGVGKTTMTVAAGSAIARERGD
                     RVIAVDVDTDLGNLSSRFHEHGGPRANIEALSSLQNAGSYSSVRIFTVQNDDRLELLA
                     AQNDPRSSYTLNSQDFESAMKILKMHYNVVLLDCGTSITSPLFATIAKQVDSLVVVAP
                     QDAPGLNGAWSTLSWLQAHGFGRLLPRTVVALDATAKGKPMVDLDEVEATFREQISEV
                     IRVPYDAHLAEGGGIDFATLNKRTRKALMNLAGAVAQHYPARQPHRVRSDETGRF"
     gene            complement(3343..4191)
                     /locus_tag="VF874_16310"
     CDS             complement(3343..4191)
                     /locus_tag="VF874_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESX secretion-associated protein EspG"
                     /protein_id="HEX9177565.1"
                     /translation="MGRAMPATSYEAVEVTARQAWFLADYLHAGSYPWKLAITSPYVD
                     PSERDSFTEQCVDELTAAGVIDSRGHVRSSVAQAVKTVCQARQWLEWYTIIDADQILR
                     GVLARSSPPDAVVALRYAQMVTFTPLQLTHSEAIVAIITAGLPANQQPAPLSEFSLPM
                     DIGKAIDDRIGRGADIVETLTDLGVAERDAEIMELARTGDRITVELTAHEATNGARHQ
                     TDVSVNVVSTQVGLILISPPAGEPRAGGNSVFAPGDSFAVAMAVRDLTDRLPSGTWFP
                     EENFSI"
     gene            complement(4166..>4396)
                     /locus_tag="VF874_16315"
     CDS             complement(4166..>4396)
                     /locus_tag="VF874_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177566.1"
                     /translation="AMASDLVQELQARAAAQAPKVATTIRISSIMFQRLEVQTKRWGI
                     TNSYLIERALEPVLRELEQAEVISDGQGNAGD"
BASE COUNT          741 a         1453 c         1503 g          699 t
ORIGIN      
        1 ccgtcgcagt cgaccaggcc gtcggctgcc gggtcgatgt agtcgcctcc ggggttgagg
       61 ttcgccagcc gcggctgagg tgtcaccccg gagtcgatta cggcgaccgc tactcccctg
      121 ccgcgtgagg tcttccatgc ctcaggcagg ttcagcatgt cctggctggg cggcaccgcc
      181 gcagggtcga agccgggcag ggcgccgaag aacgaacatt gcccgctctg gcgcatctcc
      241 tggtcgggcc cgggtggacc gtccgcaggc agcgcggcgg gatcgacgac cggcatcgac
      301 agcgcccagg cctgcggcgg cgggctgcac aacaccagca gcgcgatggc cgcggcgatg
      361 gaaaccagtt ggcgcatcat cagatagccc ctcggttgcg gatcaggctg accaggccga
      421 cgagccacaa catcgccggc aggatcgtga tcagcagcat gttctcgaac aaatccagcc
      481 agcgccggcg cgggatcgac aactcgcgtg gcttgatgac ggcggccccg atcagcaccg
      541 cggtcaaacc gacgccggcc gcggccgcat agcagccggc gatcaggtga tcggtggcgg
      601 tgaacgacac cgtcaacgct gcggcgctca gaaacggggt ggccaaaaac cacagcgaag
      661 ggatcgccga atcccagatc cgcatccgca gcacggtgac ggtcgtcgtg gtcaccacca
      721 gccaccaggc caataacgac ggcgcggcgg gcagccacag caccaacacc gatccgatga
      781 ccgacagcac aactgaggcc gcgaccatac cgctctgata cgagttgcac gtcgcggtct
      841 tgcggggcag atcctcgatc tcagctagcg aggacggcgc tggtgtcggc tcgcccgggg
      901 cgggcacatt gggcaacggg aagcgcgccc acaccgccga cgcggtgggc gcattgctgg
      961 ccaccagcag cgacaccgcc agcacgccgc agcccaacgt caggtagggc aggtgccaca
     1021 ggatgcgcac cgcggccgct gcggccacgg tcgctgacac ggcgatgatc gcggtgtggg
     1081 tggctaccca actgttggtg atcgccagca acagcagtga ccacgtcacc aggcccgccg
     1141 cggccaaaaa tacccggggt gcggcgccct caccgggcag cgacgcggcc agggccagcg
     1201 ccagcggaac cgtggttgcc acacccatgc gtccggcggc ctgtgtgagc ccacgccggc
     1261 gcagcaccac agtggcgccg atgaacacga cacccagtac gcccagtgcg gccgcggccc
     1321 ataggttgta gccgtggtgc cagccggaga cggccagtgc gcacaccagc gcacccaggc
     1381 tcaacaccac cgcctgcgcc atcgcgccca gcatggcgtg ctcgaaaggt ttgaactggc
     1441 gggcggagtg aatcgccgag gcgtcggcga tgtcctcgac gacaggtggc gcggcggggc
     1501 caggaggcaa cttgcacagc acgagttgct cgccgtcgtc aatggccagc gtttccaatg
     1561 tggcgtccag gctgaacggg gtaccgccca gcggggcaag cgaatacggt ctcagcccgt
     1621 cctcacttgc gtcctcgggt gacaactcgt cgtcgtcgca cccggaggcc agcgtcgccc
     1681 ggatccgtgg aatcaattcg cggatggcca gcgtggtcgg cagggccagg tccgcggcgg
     1741 cgccatcgcc gatcacggcg acacggattt gcccagtagc agcaggatcc atcggtcaga
     1801 atctccccgt ttcatcagag cggacccgat gcggctggcg ggccggatag tgctgtgcca
     1861 cagcgccagc cagattcatc agcgccttgc gcgtgcgttt gttcagcgtg gcgaagtcga
     1921 tgcccccgcc ctcggccaga tgcgcatcgt agggaaccct gatcacctcg gagatctgct
     1981 cacggaacgt ggcctccacc tcgtcaaggt cgaccatcgg cttgcccttg gccgtggcgt
     2041 cgagggccac cactgttcgc ggcagcagcc ggccgaaccc gtgtgcctgc agccatgaca
     2101 gcgtcgacca ggcgccgttg aggccgggcg cgtcctgggg ggccaccacg acgaggctgt
     2161 cgacctgctt ggcgatcgtg gcgaacagtg gcgaggtaat cgacgtgccg cagtcgagca
     2221 ggaccacgtt gtagtgcatc ttgaggatct tcatcgccga ctcgaagtcc tggctgttca
     2281 gcgtgtacga cgaccgcgga tcattctgtg cggcaagcaa ttccagccgg tcgtcattct
     2341 gcacagtaaa tatccgaacg cttgagtagc tgccggcgtt ctgcaaggac gacagcgcct
     2401 caatgttggc gcgggggcct ccgtgctcat ggaagcgcga cgagaggttg ccgagatcgg
     2461 tatcgacgtc cactgcgatc acccggtcgc cacgctcgcg ggcgatcgcg ctgcccgcgg
     2521 cgacggtcat ggtcgtcttg cccaccccgc ccttggcgtt gacgaacgcg accacataga
     2581 cgtcgaccaa agagctgcgg atgcgccgga tcagctcctc ggcctgcacc tgtgcggcgc
     2641 tgggacccgg agtgatcagt ccgaacgtca tcttcgagac gaccttgcgc cagcccttgc
     2701 cggcaggtcg gcggcgggaa gacaccacgc gctcatgctc tgtcccggga ccatagccgc
     2761 cgccgggacc gaacggcgct gcaccatcac ccaacgggcc accgaacaag gcctgaggac
     2821 tcgacactgg ccgttgcggg ttgcgcggcg gatgggacgc cggccggcct tgttgcggcg
     2881 agtgctcctg cgcggcaggc ggatcagtga agaacccacc gacggacgga tcccagccgg
     2941 atgcggcgtc gggctgcgag agcatcgccg tgttgtcgtc gggctcctgc gagcttggct
     3001 gccacccggt cgccgcgttg ggtgccgcgg cgtttgggtg gggctgggaa ctgggctgat
     3061 aaggctggtg cggccgtggt gggcggcccg cgtgctgcgg ggccggcccg gccggcggcg
     3121 cagtggcaga ccggcgctca tcggccgggt gttgcggctg gggcagtcgg cgctgcggac
     3181 cggtatcggc tgccgtcggt gacgacggcg gccgctggtt ctcctgcggc gctggggttc
     3241 gccgctgctg ttcgggcgac tgctcgggtc gcggctcgtg gtcgggggcg aagaaatcag
     3301 agggtgtgct catcgtttcg tatccgcttt cggttgcaat ggtcagatgc tgaagttttc
     3361 ctcgggaaac catgtgccag aaggtaatcg gtcggtcaga tcacgcaccg ccatcgcgac
     3421 ggcaaaggag tcgccggggg cgaacaccga gttgccaccg gcgcgcggct cccctgccgg
     3481 tgggctaatg agaatcagcc caacctgcgt gctgacgacg ttgacactga catcggtttg
     3541 atggcgcgcg ccgttggtcg cctcgtgggc ggtgagttcg acggtgatgc gatcgccggt
     3601 gcgagctagc tccatgatct cagcgtctcg ctcggcgacc ccgaggtcgg tcagcgtctc
     3661 cacgatgtcg gcgccgcggc cgatgcggtc gtcgatcgcc ttgccgatgt ccatcggcaa
     3721 cgagaactcg ctcaacggtg ccggctgctg attggcaggc agcccggcgg tgatgatcgc
     3781 cacgatcgcc tcactgtggg tcagctgcag cggggtgaac gtcaccatct gcgcatagcg
     3841 cagcgccacc accgcatccg gcggtgacga gcgggccagc acgccccgca ggatttggtc
     3901 ggcgtcgatg atggtgtacc actccagcca ctggcgggcc tggcataccg tcttaacagc
     3961 ctgggccaca gacgatctca cgtgacctcg ggagtcgatg accccggctg cggtgagttc
     4021 atcgacgcac tgctcggtga aggaatcacg ctcagagggg tcgacgtagg ggctggtgat
     4081 cgccagcttc cagggatagg acccggcgtg cagatagtcg gcgagaaacc atgcctgccg
     4141 ggcggtcacc tccaccgcct cgtagctagt cgccggcatt gccctgccca tcagatatca
     4201 cttcagcctg ctccaactcc cgcagcaccg gctccagtgc gcgctcgatg agataggagt
     4261 tggtgatccc ccaccgcttg gtctggacct cgagccgttg aaacatgatg ctgctgatcc
     4321 ggatcgtggt ggcgaccttg ggtgcctggg ccgccgcgcg ggcctgcaac tcctgtacca
     4381 gatctgatgc catagc
//
LOCUS       DASWBF010000444         6372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_252936,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000444 DASWBF010000000
VERSION     DASWBF010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252936"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VF874_16320"
     CDS             complement(<1..875)
                     /locus_tag="VF874_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9177567.1"
                     /translation="MLTEVASVRCSSRKRSSATNVTMVQAINRALHDEMAADERVLVF
                     GEDVAKLGGVFRVTEGLVETFGEPRCFDTPLAESALIGIAIGLALRGFVAVPEIQFDG
                     FSYPAFDQIVSHLAKYRMRTLGEIDMPVTVRVPSFGGIGAVEHHSESTETYWLHTAGL
                     KVVVPSTPADAYWLLRHAISSRDPVIYLEPKRRYWSRGDIDTSTPGLPIGRAAVRRSG
                     DHVTVLTYGGLVQTALSAADMAAAHHDWQLEVVDLRSLNPLDFDTVAASIRKTGRAVV
                     MHEGPRTLGFGAELAA"
     gene            complement(928..2001)
                     /gene="pdhA"
                     /locus_tag="VF874_16325"
     CDS             complement(928..2001)
                     /gene="pdhA"
                     /locus_tag="VF874_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX9177568.1"
                     /translation="MPTTVDLEPVRLIGPDGLPTSDGRYNRALPDETLAWLYELMVVT
                     RDLDTEFVNLQRQGELALFASCRGQEAAQIGSAACLRKTDWLFPQYRELGVFLLRGIA
                     PAKIGAVWRGIWHGGLEFTRKRCAPIAIPIGTHGLHAVGAAMAAQRLGEDSVTVAYLG
                     DGATSTGDAHEALNVASVFAAPCIFFVQNNQWAISVPVSRQHAGPSIAHRAIGYGIPG
                     IRVDGNDPLACYAVMADAADRARDGGGPTLIEAITYRMGPHTTSDDPTRYRSQAEVEH
                     WAALDPITRYRNYLRHVGVWNARLDDRVQARSARMRNELRTAVVEAEDIDVIEMFDTT
                     YADITPNLADQRQQLLSELAKEV"
     gene            complement(2271..3431)
                     /locus_tag="VF874_16330"
     CDS             complement(2271..3431)
                     /locus_tag="VF874_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177569.1"
                     /translation="MSDFLSTGTLPDHYQQLAKTLRDFAQSVVAPVAARHDAEHSFPY
                     EVVSGMADMGLFGLPFPEEYGGMGGDYFALCLALEELGKIDQSVAITLEAGVSLGAMP
                     VYRFGNEAQKQEWLPQLTSGRVLAAFGLTEPGGGTDAGATKTTARLDDGHWVINGSKQ
                     FITNSGTDITKLITVTAVTGNADGRKEISSILVPVPTPGFTAEPAYNKVGWNASDTHP
                     LSFDDVRVPQENLLGERGRGYGNFLRILDEGRIAIAALSVGAAQGCVDECVKYSKERE
                     AFGRPIGRNQAIAFKIARMEARAHTARTAYYDAAALMLSGKPFKKQAAIAKMVASEAA
                     MDNARDGTQIFGGYGFMNEFAVARHYRDSKILEIGEGTTEVQLMVIAREVGL"
     gene            complement(3457..5454)
                     /locus_tag="VF874_16335"
     CDS             complement(3457..5454)
                     /locus_tag="VF874_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX9177570.1"
                     /translation="MTFHTVLVANRGEIAVRVIRTLRSMGIRSVAVYSDADAGARHVT
                     EADVAARIGPAPARDSYLNIDAVVDAARRTGAQAVHPGYGFLSENSEFAAALDAAGIT
                     FIGPPTAAIKTMGDKIAAKAAVAEFGVPVVPGIARPGLADAELVAAATEVGYPVLVKP
                     SAGGGGKGMRVVADPADLPGALTSARRESAAAFGDDTLFLERYVLRPRHIEVQVLADG
                     YGNVVHFGERECSLQRRHQKVIEEAPSPLLDDATRARIGAAACDTARSVDYTGAGTVE
                     FIVSADRPDEFFFLEMNTRLQVEHPVTEMVTGWDLVEWQVRIAAGEKLPVTQDDIALH
                     GHAIEARVYAEDPGRGFLPTGGPVLDVVEPQMPGIRVDSGLAAGIDVGSDYDPMLAKV
                     IAHGADRAAALGLLDRALADTAVLGVVTNVEFLRFLLADPDVVAGRLDTGLLDRRVGE
                     FTSAPPTDDQLIAAGAYRWLTVWGAATNDLWTQPSGWRVGGDAPTSIRLHTGERTDHV
                     RITGQPGHATARVEDGENRSLTAVLDGSKLALTLDGLRSEYIVAADRGHIWLAGSAGT
                     VEVAEVQEAAVRPDDEHSGDAELNSPMPGTVVAVGVDPEDSETPVTAGTVVVTVEAMK
                     MEHALTAPIDGVAELLVDVGDQVKVGQPLARITQKEEEPAR"
     gene            complement(5459..>6372)
                     /locus_tag="VF874_16340"
     CDS             complement(5459..>6372)
                     /locus_tag="VF874_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9177571.1"
                     /translation="GGGDLHSKTSGVTDHLAHDDRDALRIVRRIVSTLGPRQPYLWDV
                     APTVDAATDQTELYDVVPIDSRVPYDVHDVVTRIVDGGEFAEFKPDYGATLVTGFARI
                     HGHPVGIVANNGVLFSESALKGAHFIELCDKRMVPLLFLQNISGFMVGRDYEAGGIAK
                     HGAKMVTAVACARVPKLTVVIGGSYGAGNYSMCGRAYSPRFLWMWPNARISVMGGEQA
                     ASVLATVRADALEAAGSSWSADEQEAFKAPIRQQYEEQGNPYYSTARLWDDGVIDPAD
                     TRTVLGLALSVVGQAPVESVSYGVFRM"
BASE COUNT         1007 a         2202 c         2065 g         1098 t
ORIGIN      
        1 gctgcaagtt cggcgccgaa gcccagtgtg cgcggcccct cgtgcatcac cacggcccgg
       61 ccggtcttgc ggatcgacgc ggcgacggtg tcgaagtcca gcgggttcaa cgaccggagg
      121 tcgacgacct cgagttgcca atcatggtgg gcagcagcca tgtcggccgc gctcaacgcg
      181 gtctgcacca gaccgccgta agtcaggacc gtgacgtgat cgccgctgcg gcgcaccgcc
      241 gcccgaccga tcggcagacc cggtgtgctg gtgtcgatgt ctccgcgact ccagtagcgg
      301 cgtttcggct ccaggtagat caccgggtcc cgactggaga tcgcgtgtcg caacagccaa
      361 tacgcgtcag cgggtgtgga cgggacgacg accttcaggc ccgcggtatg cagccagtag
      421 gtctcggtgg actccgaatg atgctcgacg gcgccgatcc cgccgaacga cggcacccgc
      481 acggtgaccg gcatgtcgat ctccccgagc gtgcgcatgc ggtacttagc caggtgactg
      541 acgatctggt cgaacgcggg atagctgaag ccgtcgaact ggatttccgg caccgcgacg
      601 aatcctcgca gcgcaagtcc gatggcgatc ccgatcaatg ccgattcggc cagcggagtg
      661 tcgaaacacc ttggttcccc gaaggtttca accaggcctt cggtcacccg gaacacgccg
      721 cccagcttcg cgacgtcctc gccgaacacc agcacccgct cgtcggcggc catctcgtcg
      781 tgcaacgccc ggttgatggc ctgtaccatc gtcacgttcg tcgccgatga gcgcttgcgc
      841 gaagagcaac ggactgacgc cacctcggtc agcatcgcgc cgatcggttc cggtgtggcc
      901 gtgggccgat ccatcatctg cgtcacatca gacctccttc gccaactcgc tcagcagctg
      961 ctgtcgttga tctgcgagat tgggggtgat gtcggcgtag gtggtgtcga acatctcgat
     1021 cacgtcgatg tcttccgctt cgacgaccgc ggtgcgcagt tcgtttcgca tgcgggcaga
     1081 tcgcgcctgc acacggtcgt ccagtcgcgc gttccacact ccgacgtggc gaagatagtt
     1141 gcggtaccgc gtgatcgggt ccagggccgc ccagtgctcg acctccgcct gggagcggta
     1201 tcgtgtcggg tcgtccgatg tggtgtgcgg tcccatccgg taggtgatcg cctcgatcag
     1261 cgtaggccca ccaccgtcgc gcgcccgatc cgcagcgtcg gccatcaccg catagcaggc
     1321 cagcgggtcg ttgccgtcga cacgaatccc ggggatcccg tagccgatag cgcggtgggc
     1381 gatcgacgga ccagcatgct ggcggctcac cggcaccgag atcgcccatt ggttgttctg
     1441 cacgaagaag atgcacggtg cggcgaatac cgaagcgaca ttgagcgctt catgcgcgtc
     1501 gcccgtgctg gttgcgccgt cgccaaggta cgccaccgtc accgagtcct cgccgagacg
     1561 ctgcgcggcc attgcggccc cgaccgcatg cagcccgtga gtgccgatcg gtatcgcaat
     1621 cggagcgcag cgcttacggg tgaattccag tccgccgtgc cagattccgc gccagaccgc
     1681 gccgatcttg gcgggcgcga tcccgcgcag cagaaacacc ccgagttcgc ggtattgcgg
     1741 aaacagccag tccgtcttgc gaagacacgc tgccgacccg atctgcgcgg cttcctgccc
     1801 gcgacacgac gcgaacaacg ctagctcccc ctggcgctgc aggttcacga actcggtgtc
     1861 cagatcgcgg gtcacgacca tcaactcgta cagccacgcc agggtttcgt cgggcagcgc
     1921 acggttgtat ctgccgtcgg atgtcggcag accatctgga ccgatcagcc tgaccggttc
     1981 caaatcgact gtcgtcggca cgccgcctcc tcatatccgg cccttgggag acgccttcgg
     2041 cggcgacttc cgacctgagc ggcgtcactg cacgacgggt ctcagcaaac ccgcacacac
     2101 gcccacgtgg gggtccggcg gtgcgtccgc cgaaacccgc cgtagcactg gcgctgccgc
     2161 cattatgcgc tcgaaaccga ttaccccgcc ggatcacgcg gcgcgggcac agcgtgtcgt
     2221 gaccagtgct cagtcgccgc gtgcagcgtt accggacagc aaccttttcg ttacagaccg
     2281 acctcgcggg cgatcaccat cagctgcact tccgtggtgc cctcaccgat ttcgaggatc
     2341 ttgctgtcgc ggtagtgccg ggccacagcg aactcgttca tgaacccgta cccgccgaag
     2401 atctgcgtgc cgtcgcgggc gttgtccatg gccgcttcac tggccaccat cttggcgatc
     2461 gcggcctgct tcttgaacgg cttgcccgac agcatcagcg cggccgcgtc gtagtacgcg
     2521 gtccgtgcgg tgtgcgcgcg cgcctccatc cgcgcgatct tgaacgcgat cgcctggttg
     2581 cggccgatcg gccgaccgaa tgcctcccgc tccttggagt acttcacgca ctcgtcgacg
     2641 cagccttgcg cggcgccaac ggacaacgcc gcgatggcga tccggccctc gtcgaggatg
     2701 cgcaggaagt tcccgtagcc acgaccgcgc tcccccagca ggttctcctg cggtacccgg
     2761 acgtcgtcga agctcaacgg atgtgtgtcc gacgcgttcc agccgacctt gttgtaggcc
     2821 ggctcggccg tgaacccagg cgtgggcact ggtacgagga tcgacgaaat ctctttcctg
     2881 ccatcggcat tgcccgtcac cgcggtgacg gtgatcagct tggtgatgtc ggtgcccgag
     2941 tttgtgatga actgcttgct gccgttgatc acccagtgcc cgtcgtcgag ccgcgcggtg
     3001 gtcttcgtcg cgcctgcgtc ggttccgccg ccgggttcgg tcaacccgaa cgccgcgagc
     3061 acccgcccgc ttgtcaactg cggcaaccac tcctgtttct gcgcctcgtt tccgaagcgg
     3121 tacaccggca ttgcgcccag cgacacgccg gcctccaacg tgatcgccac gctctggtcg
     3181 attttgccga gttcctccag agccaggcac agtgcgaagt agtcgccgcc catcccgccg
     3241 tactcttcgg ggaacggcag gccgaacagc cccatgtcgg ccatgccgga gacgacctcg
     3301 tacgggaacg agtgctcggc gtcgtggcgt gctgccaccg gcgcgaccac gctttgggcg
     3361 aagtcgcgca acgtctttgc gagttgctgg tagtggtccg gcagggtgcc agtggacaga
     3421 aagtcgctca tcactgtctc ctcttcgcgc gagcgctcat cgggcgggtt cttcttcttt
     3481 ctgggtgatc cgggccagcg gctggcccac cttgacttgg tcgccgacat cgacgagcag
     3541 ttcggcgacg ccgtcgatcg gcgcggtcag ggcatgttcc atcttcatcg cttcgacggt
     3601 cacgaccacc gtgccggcgg tgacgggtgt ttcggagtct tcggggtcga cgccgacggc
     3661 gacgacggtg ccgggcatcg ggctgttgag ctccgcgtcg ccgctgtgct cgtcgtcggg
     3721 gcgcacggcg gcctcctgca cctcggcgac ctcgacggtc cccgcggagc cggccagcca
     3781 gatgtggccc cggtcggcgg cgacaatgta ctcgctgcgc agaccgtcca gagtgagtgc
     3841 gagtttcgat ccatcgagaa ctgcagtgag agagcgattt tcgccatcct caacgcgggc
     3901 cgtggcgtgt ccgggttgcc cggtgatgcg gacgtgatcg gtgcgttcgc cagtgtgcag
     3961 cctaatgctg gtcggggcgt cacccccgac gcgccacccg gaaggctgcg tccacaggtc
     4021 gttcgtcgcg gcgccccaca ccgtcagcca ccgatacgcc ccagcggcga tgagctggtc
     4081 gtccgtcggt ggcgcggagg tgaattcgcc gaccctgcgg tcgagcagac cggtgtcgag
     4141 gcgaccggcg accacatcgg gatcggccaa caggaagcgg aggaactcga cgttggtcac
     4201 caccccgagc accgcggtgt cggccaaagc gcggtcgagc agtcccagtg ctgcggcgcg
     4261 gtcggcgccg tgggcgatca ccttcgccag catcgggtcg tagtcactgc cgacgtctat
     4321 gccggcggcc agaccggaat cgacgcggat gccgggcatc tgaggttcga cgacgtcgag
     4381 caccgggcca ccggtgggca ggaagccgcg tcccgggtcc tcggcgtaca cccgcgcctc
     4441 gattgcgtgg ccatgcagcg caatgtcgtc ttgcgtgacg ggaagcttct cgccggccgc
     4501 gatccgaacc tgccactcga ccaggtccca cccggtgacc atctcagtca ctgggtgctc
     4561 cacctgcagg cgggtgttca tctcgaggaa gaagaactcg tcgggccggt ccgcggagac
     4621 gatgaattcc actgtgccgg cgccggtgta gtcgacgctg cgggcggtgt cacacgccgc
     4681 ggccccgatg cgggcccgcg tcgcgtcgtc gagcagcggt gacggcgcct cctcgatcac
     4741 cttctggtgg cgccgctgca ggctgcactc gcgttccccg aagtgcacga cgttgccgta
     4801 gccgtcggcc agcacttgca cctcgatgtg cctggggcgc agcacgtatc gctcgaggaa
     4861 cagggtgtcg tcgccgaacg cggcagccga ttcccggcgg gcggaggtca gagcacccgg
     4921 caggtccgcc gggtcagcca ccacccgcat gcccttgcca ccgccaccgg ccgacggctt
     4981 caccagcacg ggatacccga cttcggttgc cgcggctaca agttcggcat cggccagccc
     5041 gggacgggcg atgccgggca ccaccggcac cccgaactcg gccaccgcag ccttcgcagc
     5101 gatcttgtcg cccatcgtct tgatcgccgc ggtcggtggc ccgatgaacg tgatacctgc
     5161 cgcgtcgagt gcggccgcga attccgagtt ctccgagagg aatccgtagc ccgggtgcac
     5221 cgcctgcgca ccggtgcgcc gtgccgcgtc gacgacggcg tcgatgttca ggtagctgtc
     5281 gcgggcaggc gctggcccga tccgcgcggc cacgtcggct tcggtgacat gacgcgcgcc
     5341 ggcgtcggcg tcgctgtaca cggcgaccga ccggatgccc atcgaccgca gcgtccggat
     5401 gacccgcacc gcgatttcgc cgcggttggc caccagcacc gtgtgaaagg tcatcgcgtc
     5461 acatccggaa cacgccgtag gagaccgact cgaccggcgc ttggcccacc accgaaagtg
     5521 ccaggcccag aacggttctg gtgtcagcag gatcgatcac accgtcgtcc cacagccggg
     5581 ccgtcgaata gtacgggttg ccctgctctt cgtactgctg gcgaattggc gccttgaacg
     5641 cctcctgctc gtcggccgac cacgaactgc cggccgcctc tagcgcgtcg gcgcggacgg
     5701 tggccaacac cgaggcggcc tgctcgccgc ccatcaccga gatccgcgcg ttcggccaca
     5761 tccacaggaa gcggggcgag tacgcgcgcc cgcacatcga atagttgcct gccccatacg
     5821 aaccgccgat caccacggtc agcttgggca cccgcgcgca tgccaccgcg gtgaccatct
     5881 tggcgccgtg tttggcgatg ccgccggctt cgtagtcgcg gcccaccatg aacccggaga
     5941 tgttctgcag gaacagcagc ggcaccatgc gcttgtcgca cagttcgatg aaatgcgctc
     6001 ccttcaaagc cgattcactg aacagcacac cgttgttcgc gacgatgccg accggatgcc
     6061 cgtggatacg cgcgaagccg gtgaccagtg tggcgccgta atccggcttg aactcggcga
     6121 attcgccgcc atcgacgatg cgcgtgacga cgtcgtgcac gtcgtaggga acgcgcgagt
     6181 cgatcggcac gacgtcgtag agctcggttt ggtcggtggc cgcgtcgacg gtcggcgcga
     6241 cgtcccacag gtagggctgc cgcgggccta gagtcgacac gatccggcgc acgatccgca
     6301 gcgcgtcgcg gtcgtcgtgc gccaaatggt cggtgacacc ggaggtcttg gagtgcaggt
     6361 cgccgcctcc ga
//
LOCUS       DASWBF010000445         5131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_827044,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000445 DASWBF010000000
VERSION     DASWBF010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827044"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VF874_16345"
     CDS             <1..743
                     /locus_tag="VF874_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX9177572.1"
                     /translation="LNPAFDDVIARGMEKRRDFRFASAGELARAATAAASPRPTTTRG
                     FPRQWPDTAATSYSPHGGQWSPPKAAKRGFGPVQIALAVAAVAVLAAAGAAVVWLAIR
                     GDSGTNVAQSTGAVPLTTTEGTTTTSTTTTTQTSVNRLPATDDSGFIGYPGARCDQGN
                     DPAALARTTQSVLVVCRAGPGSYYYRGVRLSDNAGIELSNAVRSSGGFDVTNPTDGTR
                     YEIRPTSLTIVQPDGQMFSEPMIEYASS"
     gene            761..1516
                     /locus_tag="VF874_16350"
     CDS             761..1516
                     /locus_tag="VF874_16350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9177573.1"
                     /translation="MTSTSPDAIRAETVMITGHGGDVIEAYQAIPLAPGRRGGVVWIH
                     HMPGYDRETKEFVRRLAVAGYHTICPNLYWREAPGADPDDAAAASRAAGGVPDERLVG
                     DVAGAVEHLRALEGANGKIGVIGHCSGGRHTVLVACSMKVDAAVDCYGAFVVEDAPEG
                     MPKTMKPILDLVPNLSCPLLGLFGNDDIYPTPQSVATLDAELTRCGKDHQFHSYGGAG
                     HSFFSVDRPAYRAEAAVDGWRRIEEFFAKHLAG"
     gene            1520..1792
                     /locus_tag="VF874_16355"
     CDS             1520..1792
                     /locus_tag="VF874_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HEX9177574.1"
                     /translation="MCTYLTEKIAVEGSGKGATGWFSATEATVYVDHPVHAPYGHTVN
                     VDVLSPALGTSARVALELTEETALALADAIRRAVASAPTGIASRDQ"
     gene            complement(1786..2432)
                     /locus_tag="VF874_16360"
                     /pseudo
     CDS             complement(1786..2432)
                     /locus_tag="VF874_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
     gene            complement(2442..3281)
                     /locus_tag="VF874_16365"
     CDS             complement(2442..3281)
                     /locus_tag="VF874_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177575.1"
                     /translation="MELSLTDRTYLVTGGGSGIGKGVAAALVNAGARVMIVGRNADRL
                     AGAVADIEASAGDSGGAVRYEPADITNEDEIARAVEAATAWSGRLHGAVHCAGGSESI
                     GPITQLDSEAWRRTVDLNVNGTMYVLKHSARELVRGGGGSFVGISSWAASNTHRWFGA
                     YGPTKSAIDHMMQVAADELGASWVRVNGIRPGLIRTEMAEPIFQAPDVRDDYVINTPL
                     PRAGEVEDVAMLALFLLSDAASFITGQVINVDGGISLRRGPDFTAMFEPMYGAEGLRG
                     VVS"
     gene            3452..3808
                     /locus_tag="VF874_16370"
     CDS             3452..3808
                     /locus_tag="VF874_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="intersectin-EH binding protein Ibp1"
                     /protein_id="HEX9177576.1"
                     /translation="MAHFEFPARRLIVAGGFAAAIAISPAIAVFVGPSPAVGTPNVSC
                     PAGESEDPYTFSCVPELVPTGVASPPVAANQPVGPPGEQNLTACSGTHESECLEGVLY
                     GPAPVPQVESSVEQSP"
     gene            3844..4008
                     /locus_tag="VF874_16375"
     CDS             3844..4008
                     /locus_tag="VF874_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177577.1"
                     /translation="MPAKSDPADIGDVEPLADSTARQARRVVAAYATDADECRIFLSM
                     LGIGPAKNGS"
     gene            4009..>5131
                     /locus_tag="VF874_16380"
     CDS             4009..>5131
                     /locus_tag="VF874_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX9177578.1"
                     /translation="MSSSAGKADSTPRGRSSEFVVVANRLPVDLERLPDGTTTWKRSP
                     GGLVTALEPLLRRRSGAWIGWPGVPDADDEPTVEDGLQLFPVRLSEEDVAEYYEGFSN
                     ATLWPLYHDVIVKPIYHRDWWDRYVDVNRRFAEAASRAAAKNATVWVQDYQLQLVPKM
                     LRELRPDLTIGFFLHIPFPPIELFMQLPWRTAIVEGLLGADLVGFHLPGGAQNFLILS
                     RRLVGANTSRGSIGVRSRIGEVKYGSRVVRVGAFPVSIDSADLDHKGKERGVRHRARE
                     IRAELGNPRKVLLGVDRLDYTKGIDVRLRAFSELLAEGRAKRDDTVFVQLATPSRERV
                     ESYKALREDIERQVGHINGEYGEVGHPVVHYLHRPVAREE"
BASE COUNT          900 a         1744 c         1654 g          833 t
ORIGIN      
        1 ggctgaatcc cgcattcgac gacgtcatcg cccggggaat ggagaagcgc cgggactttc
       61 ggtttgcctc cgcaggtgag cttgctcgcg cagccaccgc cgccgcgtcg ccgcggccga
      121 ccaccacccg cgggttccct cggcagtggc ccgacacggc ggccaccagc tactccccgc
      181 acggtggtca gtggagccct cccaaagcgg cgaagcgggg tttcggacca gtacagatag
      241 cgctggctgt cgccgcggtc gcggtgttgg ctgcggctgg cgccgcagtg gtgtggctgg
      301 ccatcagagg tgattcgggc accaacgtcg cgcagtcgac cggcgcagtc ccgttgacca
      361 ccacggaagg gacaacgacg acgtcgacaa ccacgacaac ccagacctcc gtcaaccgcc
      421 tccccgccac cgacgacagc ggcttcatcg gctatccggg tgcgcgctgc gatcagggga
      481 acgacccggc cgcattggcg cgcaccacgc agtcggtgct cgtcgtctgc cgtgcgggac
      541 cgggaagtta ctactatcgc ggcgtacggc tcagcgacaa cgccggcatc gaactctcca
      601 atgcggtgcg ctcgtccgga gggttcgacg tgaccaaccc gaccgacgga acgcgatacg
      661 aaatacggcc gaccagtctg acgatcgtgc agccggacgg tcagatgttc tctgagccga
      721 tgatcgaata cgcgtcgagc tgacgggaag ggctaccgcg atgacatcca ccagtccaga
      781 cgcgatccgc gcggaaacgg tgatgatcac gggccacggc ggtgacgtca tcgaggcgta
      841 ccaggcgatt ccgcttgcac cgggccggcg gggcggcgtg gtgtggattc accacatgcc
      901 cggatatgac agggagacaa aagaattcgt ccgccgtctc gccgtcgccg gctaccacac
      961 gatctgcccc aacctgtact ggcgcgaggc gcccggagca gatccggacg acgcagcggc
     1021 cgcgtcccgc gccgccggcg gcgtgccgga cgagcggctg gtcggcgatg tcgctggtgc
     1081 cgtcgagcac ctgcgcgctc tcgagggtgc caacgggaag atcggcgtga tcgggcactg
     1141 ctccggaggc cggcacacgg ttctggtcgc gtgttcgatg aaagtcgatg cagcggtgga
     1201 ctgttacggc gcgttcgtgg tcgaggacgc gcccgagggc atgcccaaga ccatgaaacc
     1261 gatccttgac ctcgtgccaa acctgagctg cccgttactc ggcctgttcg gcaacgacga
     1321 catctacccg accccgcagt cggtggcgac gctcgacgcc gagctgacac gctgcggtaa
     1381 ggaccaccaa ttccattcct acggcggagc gggccattcg ttcttctcgg tcgaccgacc
     1441 cgcgtaccgc gcggaggcgg cggtcgacgg ttggcggcgg atcgaagagt tcttcgcaaa
     1501 gcacctggcg ggctgaatga tgtgcactta tctgacagag aagatcgcgg tggagggcag
     1561 cggcaaggga gccaccggct ggttctccgc taccgaggcg accgtgtacg tcgaccaccc
     1621 cgtacacgcg ccgtacgggc acaccgtgaa cgtcgacgtg ctcagccctg cgctcggaac
     1681 gtctgcgcgt gtggcactcg aactcaccga ggagaccgcg ctcgcgttgg ctgacgcgat
     1741 ccgccgcgcg gtcgccagcg cacccaccgg tattgcgtcc agagatcaat agcgctcagg
     1801 cgcaggaaga tcggtccact ccgccgacac cgacgacgtc caccgtgggg ttcgccggtc
     1861 gacgaacgcg gccacgccct ctcgggcgtc ggcgctgccc atcacgcggc gaggcccaaa
     1921 cccggtgcct cccgtccgag gaacgtacgc cctgtcagca acaggtccgc cgccacggcc
     1981 aacccggcca gatgcgggat cgtccagtgc gacatgcagt ccggaatgac cccgcgacgc
     2041 acctgcacca caccgcatcg ggcgtcctcg gcgaagacgc ggatgtccgc ctgaagcgcg
     2101 atcgtgaggc cgatgccgat cgcatggccg ttgacggccg cgatgatggg cttgcggatt
     2161 tcgaacgcag ccggtcgaac cggcgatggc gagaagtcct cactgccagg cgaggagaag
     2221 gggttgtcgc cgctgaagtc ggcgccggca cagaacgcag ttcccgcgcc ggtcagcacg
     2281 attgcccgaa cggcgtcatc cgaatcgcag gaccgatacg cctcgccgag aacgcggccc
     2341 atttcggcgg ttcacgcatt cagccgttcc ggacggttga tagtcagaac cgctacgccc
     2401 tgcgaaacct caaccgtcag atcgctgttc atgtgtgcgg cctagctgac gacgccccgc
     2461 aacccttccg cgccgtacat cggctcgaac atcgcggtga agtctggtcc tcgccgcagg
     2521 gatatcccgc cgtcgacgtt gatgacttgg ccggtgatga agctggccgc atcgctgagc
     2581 agaaacagcg caagcatcgc gacgtcctcg acctcgccgg cgcgcggcag cggcgtgttg
     2641 atcacgtagt cgtctcggac atcgggtgcc tggaaaatcg gctcggccat ttcggtgcga
     2701 atgagtccag ggcgaattcc gttgactctc acccatgacg cgccgagctc gtcggcggcc
     2761 acctgcatca tgtgatcgat cgcggacttc gtcggcccat aggcgccgaa ccaccgatgg
     2821 gtgttgctgg cagcccacga cgagatgccg acgaacgatc ctccgccgcc gcgcaccagc
     2881 tcgcgcgccg agtgcttcag cacatacatc gtgccgttga cgttgaggtc gaccgtgcgc
     2941 cgccacgctt cggagtcgag ttgcgtgatc ggcccgatgc tctccgagcc gccggcgcaa
     3001 tgcacagcgc cgtgcagtcg gccgctccac gcggtggctg cctcgaccgc gcgcgcgatc
     3061 tcgtcttcgt tcgtgatgtc ggccggctca tagcgaaccg caccgccact atcgccggcg
     3121 gacgcctcga tatcggccac cgcgccggcg agcctgtccg cgttgcggcc gacgatcatc
     3181 acacgtgcgc cggcgttcac gagagcggcg gccacgccct taccgatgcc gctgccgcca
     3241 cctgttacga gataggtccg gtcagtgaga gacagctcca cctcaacccc ttcgtccgcc
     3301 gtcagccgga gcgtacgtca ccggccgcgt cgcgcagtat ccagctccag cccgaaaacg
     3361 cagggaacag ggggaacgtc tgcggacttc tgtcaggttt catagacttc tgcacaggca
     3421 cccatccagc gagaagacag gaagcgtgaa gatggcgcat ttcgaattcc cagctcgtcg
     3481 gctcatcgtg gcagggggct tcgccgccgc gattgccatc tcgccggcga tcgccgtctt
     3541 cgtcgggccg agcccggcgg tcggcacgcc gaacgtctcg tgccccgccg gtgagagcga
     3601 ggatccgtac acgttctcgt gcgttcccga gctcgtgcca acgggcgtcg ccagccctcc
     3661 cgtcgccgcc aaccagcccg tcggccctcc gggcgaacaa aatctcaccg cctgcagtgg
     3721 cacgcatgag agcgagtgtc tcgagggtgt gctgtacggc ccggcccccg tcccgcaagt
     3781 cgagtcgtcg gtggaacaaa gcccctagcc cagtcggcgc ccgccttcac ctagagttgt
     3841 cgtatgcccg caaaatctga ccccgctgac atcggcgacg tggaaccact ggccgacagc
     3901 accgcccgtc aggcgcgccg ggtggtcgcg gcctacgcga ccgacgcgga cgaatgccgg
     3961 atcttcttgt cgatgctcgg cattggaccg gctaagaacg ggtcgtagat gagttcctcc
     4021 gcgggcaaag ccgactcgac cccacgcgga agaagctccg agttcgtcgt cgtcgccaat
     4081 cggctgcctg tcgatctgga acggctgccc gatggcacta ccacctggaa acgtagtccc
     4141 ggcggcctcg tcactgctct cgagccactg ctgcgccgac gcagcggtgc gtggatcggc
     4201 tggccgggtg tcccagacgc cgacgacgaa ccgaccgtcg aagacgggct gcagcttttc
     4261 ccggtgcggt tgagcgagga ggacgtcgca gagtattacg aaggcttttc caacgccact
     4321 ttatggccgc tgtaccacga cgtcatcgtc aaaccgatct accaccgcga ctggtgggat
     4381 cgctacgtcg acgttaaccg ccgattcgcc gaagccgcgt cccgcgctgc ggcgaaaaac
     4441 gccaccgtat gggtgcagga ctaccagttg cagctggtgc cgaagatgct gcgcgaactg
     4501 cgaccggatc tgaccatcgg cttctttctg cacatcccgt ttcctcccat cgagctgttc
     4561 atgcaattgc cctggcgcac agcgattgtc gagggactgc tgggcgccga tctggtcgga
     4621 ttccaccttc ccggcggcgc gcagaacttc ctcatcctgt cgaggcggct cgtcggcgcc
     4681 aacacgtcgc gtggatcgat cggtgtccgt tcacgtatcg gggaggtgaa gtacgggtcg
     4741 cgggtggtcc gggtcggtgc ttttccggtc tcgatcgact ctgccgacct cgaccacaag
     4801 ggcaaagagc gcggtgtccg ccaccgcgca cgggagatcc gagcggaact cggcaacccc
     4861 cgcaaggtcc tgctaggtgt ggaccgcctg gactatacga agggcatcga cgttcgtctg
     4921 agagcgtttt ccgaactcct ggccgagggg cgcgccaagc gggacgacac cgtgttcgtg
     4981 cagttggcga cgcccagccg cgaacgcgtc gagagctaca aggcgcttcg agaggacatc
     5041 gagcggcagg tcgggcacat caacggcgag tacggcgagg taggccatcc ggttgtgcac
     5101 tacctacacc gacccgttgc gcgtgaggag t
//
LOCUS       DASWBF010000446         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_438069,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000446 DASWBF010000000
VERSION     DASWBF010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438069"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(454..909)
                     /locus_tag="VF874_16385"
     CDS             complement(454..909)
                     /locus_tag="VF874_16385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9177579.1"
                     /translation="MTCGCGCGSPGWGDIAARHHGDVLDVGCGEGLLVQRLAAVSHRV
                     IGIDPDPAEVQRAQRRLHSIDNASIEHTDFQDFTAPEQSVDVITFVASIHHQSLREAL
                     GKARQLLRPGGDELAVVGLAANKSLSEWAWSLLCMPAALVGSATAQRNP"
     gene            952..>2145
                     /locus_tag="VF874_16390"
     CDS             952..>2145
                     /locus_tag="VF874_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HEX9177580.1"
                     /translation="MSWQQIQPFLVALAIGLLLGFERERSHTRKLPAGSRSFALLSLV
                     GAVAAGFGQGAVVVGLVGVGALMALAYFRTSEEDPGTTTEIAALVAYLLGALAYTRPA
                     AAVALAVVVAGLLVSKTRIHRFAREIVSEIELEDAIKFFVVAFVILPLLPDQGLGPYG
                     VLNPAKVWLLVVLLTGIGWIGYIGVRALGPQRGLLVTGLAGGFVSASATTASMGRLSR
                     TVAGPRAPLAGALVASLATFVQLLIVIGLVDAEVLRRLWPPVVAGAIVLVGIAAFVYR
                     GATPARREDVEDTAGAADVEPIPASRPFSLRPALVLAAVLIFALLVGRWGADVLGPKG
                     AVLAAFAAGLADAHAGAVGAASLAAKGDITVDTALTAIAAALGSNLLVKSVLAFTAGG
                     RRFGLG"
BASE COUNT          300 a          756 c          724 g          365 t
ORIGIN      
        1 gaccaggcac ctaggtaggc ggcatgcggt tgccaaaccc gaagtccagc cccaccgttg
       61 caccgcgcat cgcaacaacg gcgacagatg catgcgcgcg gcgattgcag gcggtcgcgt
      121 gtgtacccca ccacggcggc aacgccccaa ccgtcaaagc caaagccaga caacgactca
      181 aagacgccgc cgatcggatg gcccgcgagc tgccgaagat ggccgtcgac gaaacgtttc
      241 cgacgcggtc aaacccgccg ccaccgggat gtgaagcatc cccggcttta tgcacacctc
      301 gttacgtgtg accggtgtgc tttccggcgt ggagtcgagc gtagtactcg gcctcggctt
      361 ccgcgggtgg gcggcgcccg agccggtgca tgagcctgcg cacgtcggat ctgaccgagg
      421 ctttcgtggg gctcggtgac cggtacgccg atatcacggg tttctctgtg cagtcgccga
      481 tcccaccagg gccgcgggca tgcagagcag gctccacgcc cattcgctaa ggcttttgtt
      541 cgccgctaaa cccacgacgg ccagttcatc accgccaggt ctgagcagtt ggcgtgcttt
      601 gcccagggcc tctcgcagcg actggtgatg aatgctggcc acgaacgtga tgacatcgac
      661 gctctgctcg ggtgcggtga agtcctggaa gtcggtgtgt tcgatcgatg cgttgtcgat
      721 cgagtgcagt cggcgctggg cccgctggac ttcggcgggg tcgggatcga tgccgatgac
      781 gcggtgcgat accgccgcca gccgttgcac cagcaggccc tcgccacaac ccacgtcgag
      841 gacgtcgccg tgatggcgtg cggcaatgtc tccccacccg gggctaccgc agccacagcc
      901 acaggtcatg cagcagaatc attccggtag agcatgaacc ggggagtcgt cgtgagctgg
      961 cagcaaattc agccgttcct ggtggcgctt gccatcgggc tgctgctcgg cttcgaacgt
     1021 gaacgcagcc acacccgcaa gctccccgcc ggctcgcgct cgttcgcact gctgtccctc
     1081 gtcggagccg tcgccgccgg cttcggccag ggggccgtgg tggtcggcct cgtcggggtc
     1141 ggggcgctca tggcgctcgc ctatttccgc accagcgagg aggacccggg gaccaccacc
     1201 gagatcgccg cgctcgtcgc ctacctgctc ggtgcgctcg cctacacccg cccggcggcg
     1261 gccgtcgccc tcgccgtggt cgtcgccggc ctgctggtgt cgaagacgcg catccatcgc
     1321 ttcgcccgcg agatcgtcag tgagatcgaa ctggaggacg ccatcaagtt cttcgtggtg
     1381 gcgtttgtaa tcctgccgtt gctgcccgac cagggactcg gaccctacgg cgtgctcaac
     1441 ccggcgaagg tgtggcttct cgtcgtgctg ctcaccggta tcgggtggat cggctacatc
     1501 ggcgtgcggg ccctcggccc ccaacggggc ctgctcgtca ccggcttggc tggcgggttc
     1561 gtgtcggcga gtgcgacgac ggcgtcgatg ggccggctca gccggacggt cgcaggccca
     1621 cgggcgccgc tcgccggcgc gctcgtcgcc agcctcgcca ccttcgtgca gctgctcatc
     1681 gtgatcgggc tcgtcgacgc cgaggtgctc cgccggctgt ggcccccggt ggttgccggg
     1741 gcgatcgtgc tcgtaggaat cgccgccttc gtctaccggg gcgccacccc ggcccgtcgg
     1801 gaggacgtcg aggacaccgc gggggccgcg gacgtcgagc cgatccccgc gagccggcct
     1861 ttctccctgc ggcccgctct tgtcctggcc gctgtgctga tcttcgccct gctcgtcggc
     1921 cgatggggag ccgacgtgct cggtcccaag ggggcggtgc tcgccgcgtt cgccgcaggc
     1981 ctcgccgacg cccacgccgg cgcggtcggc gccgcaagcc tcgctgccaa gggcgatatc
     2041 accgtcgaca ccgcgctgac agccatcgcc gccgcgctcg gatcgaacct gctcgtcaag
     2101 agcgtgctgg ccttcaccgc gggcgggcgc cgcttcggac tcggg
//
LOCUS       DASWBF010000447         7335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_438081,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000447 DASWBF010000000
VERSION     DASWBF010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438081"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VF874_16395"
     CDS             complement(<1..825)
                     /locus_tag="VF874_16395"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177581.1"
                     /translation="MDSLLSLGGRIVVVSGAGGGGIGTTVTRLAAEAGATVIAVSRSK
                     ENLDEHVAPLARRGLSVVPVAADASTDEGIAAVIDQARRAEGRLYGLVNVAGGAAPST
                     WMPSTRVSRTDWRELFTANLETAFFMSQAVAAELRTHKLPGSIVSLSSISGVNTAPFH
                     IAYGTAKAAVTAMTRTMAVELALEGIRVNAVAPGVTETAASRTYVDEDPDRDRQAIAM
                     GRRGRPEEQAGVILFLLSDLSSYVTGQTLLVDGGLDLKWSHLAADNTSLFLKDESFR"
     gene            969..2588
                     /locus_tag="VF874_16400"
     CDS             969..2588
                     /locus_tag="VF874_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9177582.1"
                     /translation="MQDARYTAATPPTAADGWRVERVAGPSRLFGANGLRTGPDGRVY
                     VAQVTGSQISALDLSTGQLDTVSAKGGDIIAPDDVAFDPSGNLYATEVMDGRVSVLDT
                     AGRIRVLRDDVPSANGIAFHAGRLFIGECREGGRLLEMDLNGGAPRVLLENVPSPNAM
                     EVGPDGLLYFPVMGANEIWRIDPDSADARSKTGAGEPQRVAGDLGVPDSVKFDSEGFI
                     VSTQVASGQVLRIDPRSGEQTVLAQLTPGLDNCTFVGDRLFVSNFTGEITEILPGGDT
                     RTVLPGGLNWPLDLAVGDDGNLYVADGTYFYVVRPDKRLQTVGMLFSPGYPGFLRGLA
                     TAGAGEFVVTTSGGQVARYRPAANETDYLATDFDQLYGVAIGPGEAIVFAELGTGRVH
                     SLRSGNVDTLASGLLDPVGVAFDPDGTPLVAESGAGRVVRLSGSAVETVVDGLQRPQG
                     ILVTDGQLYIVDAGAKELVHVDLSTGARHTIASGLPVGPPPGVVPKPLKGMPPFSGPQ
                     GPFAGITIGQDGTLFVSGDGDGSVLALHRVQ"
     gene            2620..3357
                     /locus_tag="VF874_16405"
     CDS             2620..3357
                     /locus_tag="VF874_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9177583.1"
                     /translation="MTISPADHRYLQVARALRKEIVDGVYPVGSQLPTEHELCERFAV
                     SRYTIREALRRLREDNLVSSRPRAGTLVVPRPASNSYAQDVMSINDLLAFAAGAQLTI
                     ESNAMVTIDDELAERTGLETGTEWLAVRGYRQADGASAPVCRTEYYINRSFAAVGRML
                     QRHTGPIFPLIEDLFGASVVEVQQEIAAVVLSPELADRLNTESGTAALEMRRTYKTSD
                     GEIAQVTINTHPSSRFRHSMTMRRVKG"
     gene            3360..4943
                     /locus_tag="VF874_16410"
     CDS             3360..4943
                     /locus_tag="VF874_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9177584.1"
                     /translation="MRLDGQQAADAYARGWWVHSTLADSLREAAQSTPQRTALVDGDV
                     RVDCRRLHDQATALAHQLSTRMPVGSVVSFMLPNWHEAAVVYHAATLAGMVVNPILPS
                     LRDRELRFILADAQSRMIFVPARFGGHDYAAMLTRVAAQMESPPEVIVVRGDGTEDDT
                     PYASLLEDHTPQELPALDPDAVRMILYTSGTTARPKGVLHTHNSIHALICQIRDHWLV
                     DPGDRFLVPSPIAHIGGSIYAFECPMLLGTTAVLMERWNADDAVGLMVRERCTHMAGA
                     TPFLVQMLAAAERAGTHLPDLKVFICGGASVSPSLIRRAASYFDRAVVSRVYGSTEVP
                     VTTVGAPGDLDHAADTDGRAGVADIKLAAHPAASPGEGEICAQGPQMLIGYLHPEDDA
                     ESFDADGYFRTGDLGRWVDGEYLVVTGRAKDIIIRNGENISPKEIEDVLVGHPGIAEV
                     AVVGLPDPRTGERACAVIVPATAPDPDVAALGSFLAAAGVARFKVPEQVAIWESLPKN
                     DAGKVLKHQIRAALIEGRR"
     gene            5007..5780
                     /locus_tag="VF874_16415"
     CDS             5007..5780
                     /locus_tag="VF874_16415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177585.1"
                     /translation="MQVAIVTGASSGIGFGCATKLAEMGMAVLGVGRDESRLAELKQA
                     IREPDRVATLAVDLTADDAPRRIVDAAVARWGHIDFLINNAGVGSPKPLHETDDETLD
                     YFLNLMLRAPFRLARDVIPHMPPGSAIINVTSTFAVVGGLRGGAYSAAKGGLTALTTH
                     IACQYGAQGIRCNAVAPGVTLTPMVAQRLEDERFRKINTEMTPHQRLGRVEDIASTVA
                     FLCSDGGSFINGQTIVVDGGWSSTKYLSDFALTSEWVAR"
     gene            5777..7246
                     /locus_tag="VF874_16420"
     CDS             5777..7246
                     /locus_tag="VF874_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9177586.1"
                     /translation="MNLFALLDQAASRFADRGAVYHGERQLHTWRELRDRALCLATSV
                     RQVGGPGARIAIASENCPEIVELMFAIWAAECVVVPINFKLHPREMAQILEDAGGAQV
                     FASPKIAPELAAVSNAAIETIGTESYSRWLTTTPSTPPSTDPSTLAWLFYTSGTTGRS
                     KGAMLSHRNLMAMTVAHLADFDAPNEDCSLIHGAPMSHGSGLYIPPYVLRGARQVIPA
                     SGAFDPDEFLDLCEHHPGASAFLAPTMVQRLIDTDRQCPRNLRTIVYGGGPMYVDSLK
                     KALAAFGQVFVQLYGQGEAPMTITGLRRADHLSGDDGVLGSVGYARSGVDVAVLRPDG
                     AAADIGEIGEIVCRGDVVMSGYWNNPAATAETLKDGWLHTGDMGSFDARGFLTLRDRS
                     KDVVISGGSNIYPREVEEVLVEHPGVSEAAVVGAPDAEWGEVVVAFVVGAVEPADLDA
                     HLLQRIARFKRPKRYVFVDALPKNSYGKVLKRELRNQLA"
BASE COUNT         1236 a         2447 c         2469 g         1183 t
ORIGIN      
        1 gcggaaggat tcgtctttga ggaacaacga ggtgttgtcg gcggccagat ggctccactt
       61 gaggtccagc ccaccgtcga caagcagcgt ctggccggtg acgtagctcg acaggtccga
      121 cagcaggaac aggatcaccc cggcctgttc ctcggggcgc ccgcgccggc ccatcgcgat
      181 ggcttgccgg tcacggtcgg ggtcttcgtc gacgtaggtg cgcgacgccg cagtctcggt
      241 gacgccaggt gcgacggcgt taacgcggat gccctccagc gccagttcga ccgccatcgt
      301 gcgggtcatc gcagtgaccg ctgccttcgc tgttccgtag gcgatgtgga acggcgcggt
      361 gttcactccg ctgatcgacg agagcgacac gatcgaaccc ggaagcttgt gcgtacggag
      421 ttccgcggcg acggcctggc tcatgaagaa cgcagtctcg aggttggccg tgaacagctc
      481 acgccagtcg gtgcggctca ccctcgtcga cggcatccat gtcgacggtg ccgcgccgcc
      541 cgcgacgttc acgagcccgt acaaccggcc ctcggcacgc cgggcctggt cgatcaccgc
      601 ggcgatgccc tcatcggtcg acgcgtcggc agcgaccggc acgaccgaga gccctcgccg
      661 agccagcggg gcgacgtgct cgtcgaggtt ctccttcgac cggctgaccg cgatcacggt
      721 cgccccggcc tcggctgcca gccgggtcac ggtggtgccg atgccgccgc caccggcgcc
      781 cgacactacg acgatgcggc cgcccaggct caacaaactg tccatagccg tgctaattgt
      841 ccggacaaca tacgatgctc tgcatattgc agaacactat tccgcaggga ttatcgctgc
      901 gtcaaggccg acctcgctgt caagactgag tattgtctgg actagcagca gttgataacg
      961 tccagcgcat gcaggacgcc cggtacacag ccgcaactcc cccgaccgct gcggatggct
     1021 ggcgggtgga gcgggtggcc ggacccagtc ggctgttcgg cgccaacggc ctgcgcaccg
     1081 gtcccgacgg gcgcgtgtac gtcgcgcagg tgaccggtag tcagatcagc gcactcgacc
     1141 tttcaaccgg gcagctggac accgtgagcg ccaagggtgg cgacatcatc gcgcccgacg
     1201 acgtggcctt cgatcccagc ggtaacctct acgccaccga agtgatggac ggccgggtca
     1261 gcgtgctgga caccgccggc cgcatccggg tgctgcgcga cgacgtcccc tccgcaaacg
     1321 gcatcgcctt ccacgccggc aggttgttca tcggcgagtg ccgcgagggc gggcgcctgt
     1381 tggaaatgga tctgaacggc ggcgcgccac gggtgctgct ggagaacgtc ccgtcaccca
     1441 acgcgatgga agtcggcccc gacggcttgc tgtatttccc ggtgatgggc gcgaacgaga
     1501 tctggcggat cgaccccgac agcgcggacg cgaggagcaa aacaggcgcc ggcgaaccgc
     1561 aacgcgtcgc aggtgacctc ggtgtcccgg attcggtgaa gttcgattcc gagggtttca
     1621 tcgtgtcgac ccaggtggcc agcggccagg tgctgcgcat cgatccgcgc agcggcgagc
     1681 agacggtgct ggctcagctc acacccgggc tggacaactg caccttcgtc ggtgaccggt
     1741 tgttcgtctc caacttcacc ggcgagatca ccgagatcct gcccggcggt gacacccgca
     1801 ccgtgctgcc gggcgggctg aactggccgc tggatctcgc ggtcggcgat gacggcaacc
     1861 tctacgtcgc cgacggcacc tacttctacg tcgtgcgccc ggataagaga ttgcagaccg
     1921 tcggcatgct gttctcgccc ggctatcccg ggttcctgcg cgggctggcg acggccggcg
     1981 ccggcgagtt cgtggtgacg acgtcgggcg ggcaggtcgc ccggtaccga ccggctgcca
     2041 atgaaaccga ttacctggca accgatttcg accagctcta tggcgtcgcc attggaccgg
     2101 gtgaggcgat cgtattcgcc gaactcggca caggccgagt gcactctctg cggtcgggca
     2161 acgtcgacac actggcgtcc ggactactcg atccggtcgg cgtcgcgttc gacccagacg
     2221 gcacaccgct ggtggccgag tcgggagccg ggcgggtggt ccggctttcg ggttcggccg
     2281 tcgagaccgt ggtcgacgga ctgcagcgcc cgcagggcat cctcgtgacc gacggacagc
     2341 tctacatcgt cgacgccggc gccaaggaac tggtccacgt cgacctgagc actggtgcgc
     2401 ggcacaccat cgcctccggg ttgccggtcg gtcccccgcc cggtgtggtt cccaagccgc
     2461 tcaagggaat gccgccgttc tccggcccgc agggcccgtt cgcgggcatc acgatcggac
     2521 aggacggcac gctgttcgtg tcgggtgacg gtgacggcag cgtgctggcg cttcaccggg
     2581 tccaatgacg ggccagcgag aagcgaaggc ggatcgcgca tgactatcag ccccgccgat
     2641 caccgctacc ttcaggtggc gcgcgcactg cgcaaggaga tcgtcgacgg ggtgtatccg
     2701 gtgggctcac aactgcccac cgagcacgaa ctgtgtgagc gatttgcggt gagccgctac
     2761 acaatccggg aagcactgcg ccgactgcgc gaggacaacc tcgtctcgtc tcgtccccgc
     2821 gcgggcacgc tggtggtgcc ccggccggcg tcgaattcgt atgcgcagga cgtgatgtcg
     2881 atcaacgacc tgctcgcgtt cgccgccggc gcacagctca cgatcgagtc caacgcgatg
     2941 gtcacgatcg acgacgagct tgccgagcgc accggcctgg aaacgggcac ggagtggctg
     3001 gccgtgcggg gctaccggca ggccgacggc gcgtcggcac cggtctgccg caccgagtac
     3061 tacatcaacc gctcgttcgc ggcggtcggc cgtatgctgc aacgccacac cggcccgatc
     3121 ttcccgctca tcgaagacct gttcggggcc agcgtcgtcg aggtgcaaca ggagatcgcc
     3181 gccgtggtgc tctcccccga gctggccgac cggctcaaca ccgagtcggg caccgcggcc
     3241 ctggaaatgc ggcgcaccta caagacgtcg gacggggaga tcgcacaggt gaccatcaac
     3301 acccatccct cgtcgcgctt ccgccattcg atgaccatgc gccgggtgaa ggggtgagcg
     3361 tgcgcctcga cgggcaacag gcggccgacg cgtatgcgcg tgggtggtgg gtccactcca
     3421 cgctggctga ctcactgcgt gaggctgcac agagtacacc gcaacgaacg gcgttggtgg
     3481 atggggacgt tcgtgtcgat tgccggcgtc tgcacgatca ggcgacggca ctggcgcacc
     3541 aactgagcac gcggatgccg gtgggcagtg tggtctcctt catgctgccc aattggcatg
     3601 aagccgccgt cgtctaccac gccgcgacgc tggccgggat ggtggtgaac ccgatcctgc
     3661 cgtcgttgcg ggaccgcgaa ctgcggttca tcctcgccga tgcccaaagc cgcatgatct
     3721 tcgtgccggc caggtttggc ggccacgact acgcggcgat gctgacgcgc gtggccgcgc
     3781 agatggagtc gccaccggaa gtcatcgtgg tgcgcggcga cgggaccgag gacgacacgc
     3841 cctacgcgtc gctgctcgag gaccatacgc cgcaagaatt gccggcactc gaccctgacg
     3901 cggtgagaat gattctctac acgtcgggaa cgacggcgcg gccgaaaggc gttctgcaca
     3961 cccataactc gatccacgcg ctgatttgcc agatccggga ccactggctg gtcgaccccg
     4021 gcgaccgatt cctcgtcccg tcaccgatcg cccacatcgg cggatccatc tacgcgttcg
     4081 aatgcccgat gctgctcggc acgaccgcgg tgctcatgga gcgctggaac gccgacgacg
     4141 cggtggggct catggtccgc gagcgctgca cccacatggc gggggcgacg ccgttcctcg
     4201 tgcagatgct cgccgccgcc gagcgcgccg gcacgcacct gcccgacctc aaggtgttca
     4261 tctgcggcgg cgcgtcggta tcaccgtcgc tgatccgcag agcggcatcg tatttcgacc
     4321 gcgctgtggt gagccgtgtc tacggctcga cggaagtgcc ggtcaccacc gtgggcgccc
     4381 ctggcgacct cgaccatgcc gccgacaccg acggccgcgc gggcgtcgcc gacatcaagc
     4441 tcgcggccca ccccgcggcg agcccaggcg agggcgagat ctgcgcgcag ggaccccaga
     4501 tgctcatcgg ctacctgcat cccgaggacg acgccgagtc attcgacgcc gacggctatt
     4561 tccgcaccgg agacctcggc cgctgggtcg acggtgaata cctcgtcgtg accggccggg
     4621 ccaaggacat catcattcgc aacggcgaga acatctcgcc gaaggagatc gaggatgtcc
     4681 tcgtgggcca ccccggcatt gccgaggtcg cagtcgtcgg gctgcctgac ccgcggaccg
     4741 gagaacgggc ctgcgcggtc atcgtgcccg cgaccgcacc cgatcccgac gtcgccgccc
     4801 tgggcagctt cctcgcggct gcaggtgtcg cgcggttcaa agtgcccgag caggtagcca
     4861 tttgggaatc actgcccaag aacgacgcgg gcaaggtcct caaacatcag atccgggcgg
     4921 cgctgatcga agggcggcga tgagcgcttg cgcgaagagc agagagagca gatgagcgct
     4981 tgcgcgaaga gcagagaggg cagagcatgc aagtagcgat cgtgacgggc gcaagcagcg
     5041 gcatcggttt cgggtgtgcg accaaactcg cggagatggg catggcggtg ctcggcgtcg
     5101 gcagggacga gagcaggctc gcagagttga agcaggcaat cagggaaccg gatcgtgtcg
     5161 ccactcttgc cgtcgacctg accgctgacg acgcgccccg acgaatcgtc gacgcggccg
     5221 tcgcccggtg gggccacatc gacttcctga tcaacaacgc aggcgtgggc agcccaaagc
     5281 cactgcacga aaccgacgac gaaacactgg actacttcct gaacttgatg ctgcgcgcgc
     5341 cgttccgctt ggcccgcgat gtgatcccgc acatgccgcc cggctcggcg atcatcaacg
     5401 tgacgtcgac gttcgcggtc gtcggcggac tccgtggcgg cgcgtactcg gccgccaaag
     5461 gcgggctcac agcgctgacc acccacatcg cctgccagta cggggcgcaa ggcatccggt
     5521 gcaacgccgt ggcacccggc gtcaccctca ccccgatggt ggcgcagcgc ctggaagacg
     5581 agcggttccg caagatcaac acggagatga cgccgcatca gcgcctcggg cgggtcgagg
     5641 acatcgcgag cacggtggcg ttcttgtgtt cggacggcgg cagtttcatc aacggtcaga
     5701 cgatcgtggt ggacggcggc tggagttcga cgaagtacct gtcggacttc gcgttgacct
     5761 cggagtgggt tgcacgttga atctgttcgc actgctggac caggccgcgt cacggttcgc
     5821 cgatcgcggc gcggtctatc acggtgaacg ccagttgcat acatggcgtg aattgcgcga
     5881 ccgtgcactg tgtctcgcga cctcagtgcg gcaggtcggc ggccccggcg cgcgcatcgc
     5941 gatcgccagc gagaactgcc ccgagatcgt cgagctgatg ttcgcgatct gggcggcgga
     6001 atgcgtggtc gtcccgatca acttcaagct gcatccgcga gagatggcgc agatcctcga
     6061 ggacgccggc ggcgcacagg tattcgcatc gccgaaaatc gcacccgagc tcgcagccgt
     6121 ctccaatgcg gcgatcgaaa ccattggcac agaatcgtat tcacgctggc tcaccacgac
     6181 gccgtcgact cccccgagca ctgacccgtc gacgctggcg tggctgttct acaccagcgg
     6241 aaccaccggc cggtcgaagg gcgcgatgct gagccatcgc aacctgatgg cgatgaccgt
     6301 cgcgcacctc gccgatttcg acgccccgaa cgaggattgc agcctcattc acggcgcgcc
     6361 catgtcgcac ggctcgggcc tctacatccc gccctacgtg ctgcgcggtg cccgtcaggt
     6421 gattccggcg tcgggtgcct tcgaccccga tgagtttctc gatctgtgcg agcatcaccc
     6481 cggtgccagc gcctttctcg cgccgaccat ggtgcaacgc ctgatcgaca ccgaccgtca
     6541 atgcccgcgc aatctgcgga ccatcgtcta cggcggtggc ccgatgtacg tggacagcct
     6601 caagaaagcg ttggcggcgt tcggccaggt cttcgtgcag ctctacgggc agggcgaggc
     6661 gccgatgacg atcaccgggt tgcgccgcgc cgaccacctg tccggcgacg acggggttct
     6721 cggctcggtc ggttatgcgc gatccggggt ggacgtcgcg gtgctgcgcc ccgacggcgc
     6781 cgccgccgac atcggcgaga tcggtgagat cgtgtgtcgc ggcgatgtgg tcatgtccgg
     6841 ctactggaac aatcccgccg ccaccgccga aaccttgaag gacggttggc tgcacaccgg
     6901 cgacatgggc tccttcgatg cgcgaggatt tctcaccctg cgcgaccgat ccaaagacgt
     6961 ggtgatcagc ggcgggagca acatctaccc ccgcgaggtc gaggaagtgc tggtcgagca
     7021 tcccggcgtc tcggaggccg ctgtggtggg tgccccggac gccgagtggg gtgaggtggt
     7081 ggtcgcgttc gtggtcggcg cggtcgagcc ggccgatttg gatgcgcatc tgctgcaacg
     7141 catcgcgcgc ttcaagcgcc ccaagcggta cgtgttcgtc gacgcgttgc cgaagaacag
     7201 ttacggcaaa gtactcaagc gcgaactgcg caaccagctg gcgtgagggc gacgtgagcg
     7261 actatctgct ggtccacggc gcgttctgcg gcggctgggt gtgggacgac gtggccgagc
     7321 ggttagagaa agctg
//
LOCUS       DASWBF010000448         6961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_897378,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000448 DASWBF010000000
VERSION     DASWBF010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897378"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VF874_16425"
     CDS             <1..498
                     /locus_tag="VF874_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX9177587.1"
                     /translation="FALRNKTRLAALRVKDFSKRDVMVVHTLLWPDEIRDPDFPVLDK
                     DVDIKPAELKMAEQVVDSMADDFNPDQFHDDYQDQLHELIEAKLEGGEAFPTEEKPAE
                     LDVTDDVSDLLAKLEASVRKRRTESGPKKAPAKKAARKTPAKKAAGAVKPATSSPAKK
                     AASRK"
     gene            508..696
                     /locus_tag="VF874_16430"
     CDS             508..696
                     /locus_tag="VF874_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177588.1"
                     /translation="MLRRIGYALAGITAAAALTMTPTASADDPPNPDPTIMDVLDSVL
                     ADSAPATPAPPPNATAPR"
     gene            786..1061
                     /locus_tag="VF874_16435"
     CDS             786..1061
                     /locus_tag="VF874_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177589.1"
                     /translation="MSSTARLIAAAGVALAFTMAPVAAADPADLVPVCSGDENPQLDN
                     CSSGCPENAAVSAYGTCTEVGTVPTGTGPSDQTASTGADPDVPLGPQ"
     gene            1071..1862
                     /locus_tag="VF874_16440"
     CDS             1071..1862
                     /locus_tag="VF874_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177590.1"
                     /translation="MILDRFRLDDQVAVVTGAGRGLGAAIALAFAEVGADVLISSRTQ
                     SELDDVAEKIRGLGRRAHVVVADLADTEATASLAQTAVEAFSRLDIVVNNVGGTMPNL
                     LLNTSTKDLKSAFTFNVATAHALVTAAVPLMLEHSGGGSIINITSTMGRLPGRGFAAY
                     GTAKAALAHYTRLAALDLCPRIRVNAIAPGSIHTSALEVVAGNDEMRTQLEKATPMRR
                     LGEPADIAAAAVYLASPAGAYLTGKTLEVDGGLIMPNLDLPIPDL"
     gene            1873..2946
                     /locus_tag="VF874_16445"
     CDS             1873..2946
                     /locus_tag="VF874_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX9177591.1"
                     /translation="MAIRVAQIGTGNVGRHALAGILANPQFELTGVWVSSEAKAGRDA
                     ADLAGLDRRTGVVATNDLAEVIESKPDCAVYTALADNRLPDALEDYRRLLEAGINVVG
                     SGPVFLQYPWDVIPDELIRSLEDAAQQGNSSLFVNGIDPGFANDLLPLALAGTCQSIQ
                     QVRCMEIVDYATYDSATVMFDIMGFGKPLDETPILLQPMVLSMAWGSVVRQIAAGLGV
                     TLDEIVEVYYREPAPEDFEIASGFIAKGTAAALRFEVRGLVDERPAVVLEHVTRLRDD
                     LGGDWPRPAQPGGSYRIEVTGEPSYALDLCLSSPNGDHNHADLVATAMRIVNAIPAVV
                     AAEPGIRTTLDLPLITGKALYAP"
     gene            2968..3228
                     /locus_tag="VF874_16450"
     CDS             2968..3228
                     /locus_tag="VF874_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177592.1"
                     /translation="MRIQFRYLLAAGAFAALLGAPAAAADPTCINTGDATECSSPGNV
                     EINATPPVSEFTLPYWDETFGGAYPGPYPVPYDEGSGDMGRR"
     gene            3238..3486
                     /locus_tag="VF874_16455"
     CDS             3238..3486
                     /locus_tag="VF874_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177593.1"
                     /translation="MRKAGRLAAMAALGIAAVAGAPTAAALPDCGTVAPWTMQCERGT
                     HTSINTSPNVIVNTGPFVQQPWLYPGYPVFGIGGWAVP"
     gene            3595..3849
                     /locus_tag="VF874_16460"
     CDS             3595..3849
                     /locus_tag="VF874_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177594.1"
                     /translation="MSALIPSLAIVTVTAGVVAAVAAAGRAAAVPECTNTGPTTTQCE
                     RPGNAQINTSPGYNTYDNYPWGFPWGSGITISLGGLGLGF"
     gene            3987..5216
                     /locus_tag="VF874_16465"
     CDS             3987..5216
                     /locus_tag="VF874_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX9177595.1"
                     /translation="MAATQQTDLRLRPSWYLLGPAFVAAIAYVDPGNVAANVSAGAQF
                     GFLLVWVILLANVMAGLVQYLSAKLGLVTGRSLPEAVGRRMRRRTRLAYWVQAELVAM
                     ATDLAEVVGGALALRLLFDLPLLVGGVITGIVSLLMLLIQDRRGQRVFERVITGLLLI
                     IAIGFLTSLFVNPPAPGDVVGGMLPRFDGAESVLLASAMLGATVMPHAVYLHSGLARD
                     RHGHPEGRRRVLLLRVTRWDVALAMVVAGAVNLAMLLVAATNLQGNPNTDSIEGAHAA
                     VRDALGPTVALFFAIGLLASGLASTSVGAYAGAMIMQGLLHRKIPLLLRRLITLVPAL
                     TILAIGIDPTRALVLSQVVLSFGIPFALIPLVRLTSDASVMGSDTNHRVTTALGWVVA
                     ALISLLNVGLLYLTATH"
     gene            5216..6262
                     /locus_tag="VF874_16470"
     CDS             5216..6262
                     /locus_tag="VF874_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HEX9177596.1"
                     /translation="MHSYFAYGSNLCVAQMARRCPDATNPRPATLADHDWLINQRGVA
                     TVEPLDGTHVHGVVWELSDRDLATLDSAEGVPVRYRRDRLTVHTGDGPADAWVYVDHR
                     VETGAPRPGYLERIIDGAIHHGLPQRWIEFLRRWDPSQWPRHVASKNAGPQTLSALLA
                     QPGVVEESRLRSRFGFLAIHGGGLEQMTDVIAERAADAAEASVYVVRHPDRCPHHLPS
                     ARYRADESARLAEFLDHVDVVVSLHGYGRIGRSTQMLAGGRNRELAAHLSRHLVLPGY
                     HLVTDLESIPPELRGIHPDNPVNRARGGGVHLELSPRVRGLSPRSQLPGDDGLSPVTS
                     ALVQGLAAAARAWA"
     gene            6342..>6961
                     /locus_tag="VF874_16475"
     CDS             6342..>6961
                     /locus_tag="VF874_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HEX9177597.1"
                     /translation="MKSRTPRLPGTPGIYRTRITHLRQAPVNHYFEHRSYSWYVDIDE
                     LPRLPRWLRVFASFDPRDHFAGHEGESLRQRLDAFLARHDETVTGGRITALLQPRVLG
                     HAFNPLTLYWCHDRHGVLRHVVAEVHNTHGERHAYLLPSAATPVLATKRLYASPFHPL
                     DGHYVIRAPRPDRRLDVDVSLYRGDEPAFVATVRGKRRPATITEVLR"
BASE COUNT         1156 a         2408 c         2261 g         1136 t
ORIGIN      
        1 ttcgcgctgc gcaacaaaac gcggcttgcc gcgttgcggg tcaaggactt cagcaagcgc
       61 gacgtgatgg tcgtccacac gctgctgtgg cccgacgaaa tccgcgaccc cgatttcccg
      121 gttctggaca aggacgtcga catcaagcct gccgagttga agatggccga gcaggtggtc
      181 gactcgatgg ccgacgattt caaccccgac cagttccacg atgactacca ggatcagttg
      241 cacgagctga tcgaagcgaa actcgaaggc ggcgaagcat ttccgaccga ggagaaaccc
      301 gccgagctcg acgtcaccga cgacgtctcc gatctgttgg ccaagctcga ggccagcgtg
      361 cgcaagcgcc gcaccgagtc gggcccgaag aaggcacccg cgaagaaggc cgccaggaag
      421 acgccggcca agaaggcagc cggagccgtc aagccggcga catcgagccc cgcgaagaag
      481 gctgccagca ggaagtaggc tgtggcgatg cttcgccgaa tcggatacgc actggcgggg
      541 atcacggcgg cggcggcgct gacgatgact cccaccgcgt cggccgacga cccgccgaac
      601 ccggacccga cgatcatgga cgtgctggat tcggtgctgg cggatagcgc cccggccacg
      661 cccgcaccgc cgccgaacgc cactgcgcca cgctgagaca ttcggcgtta gccgccaccg
      721 acaccgtcga ttctgttacg ttttcacacc tatactccca ggaaagaggc agtccgatgt
      781 cgatgatgtc gtctaccgct cgcctgattg ccgcggctgg cgtcgccctc gcgttcacca
      841 tggctcccgt cgccgcagcc gatccggccg atctggttcc ggtctgctcg ggcgacgaaa
      901 acccgcagct ggacaactgc agctcgggct gcccagagaa cgcggccgtc agcgcgtacg
      961 gcacgtgcac cgaagtggga acggtgccaa ccggaaccgg cccctccgat cagacggcgt
     1021 cgaccggtgc ggacccggat gtccccctcg gcccgcagta gtctgccggg gtgatcctcg
     1081 acagatttcg gcttgacgat caagtagcag tagtcacagg agcaggccgc ggacttggtg
     1141 cggcgatcgc gctggcgttc gcggaagtcg gtgccgatgt gctcatctcg tcgcgcaccc
     1201 aaagcgagct cgacgatgtc gccgagaaga tccgcgggct cggccgtcgt gcccatgttg
     1261 tcgtggccga cctcgccgac accgaggcca ccgcaagctt ggcgcagacg gccgtcgagg
     1321 cgttctcgcg tctggacatc gtggtcaaca acgtcggcgg taccatgccc aacctgttgt
     1381 tgaacaccag cacaaaggat ttgaagagcg cgttcacgtt caacgtggcg acggctcatg
     1441 cgttggtcac ggcagcggtg ccgctgatgt tggagcactc cggcggcggc agcatcatca
     1501 acatcacgtc gacgatgggc cggctgcccg gacgggggtt cgccgcctac ggcaccgcca
     1561 aggcggcgct ggcccactac acgcggctgg cagctctgga cctgtgcccg cggatccggg
     1621 tcaacgcgat cgcaccggga tccatccaca cctcggccct cgaggtcgtc gccggcaatg
     1681 acgagatgcg cactcagctg gaaaaggcga caccgatgcg gcgactgggc gaacccgccg
     1741 acatcgccgc cgccgcagtc tatttggcgt cgccggcagg ggcttacctc accggcaaga
     1801 cgctcgaggt ggacggtggt ctgatcatgc ccaacctcga tctcccgatt cccgacctgt
     1861 aaggacacat cgatggcaat acgagtagcg cagatcggaa ccggcaatgt cggtcggcat
     1921 gcattggccg gcattctcgc caacccgcaa ttcgagctca ccggcgtgtg ggtgtcgtca
     1981 gaggccaagg ccggccgtga tgcagccgac ttggcagggt tggaccggcg caccggcgtg
     2041 gtcgccacca acgacctcgc ggaagttatt gaaagcaaac ccgattgcgc cgtctacacc
     2101 gcgttagccg acaaccggct gcccgacgca ctcgaagact accggcggtt gctggaggcg
     2161 ggcatcaacg tcgtcggcag cggcccggtg ttcctgcagt acccgtggga tgtcatcccg
     2221 gacgagttga tcaggtcgct ggaagacgct gcgcagcaag gaaattcgag cctattcgtc
     2281 aacggcatcg accccggctt cgcgaatgac ttactgccgc tggctcttgc gggcacatgt
     2341 cagagcatcc agcaggtgcg ctgcatggag atcgtcgact acgcgacgta tgacagtgcc
     2401 acggtgatgt tcgacatcat ggggttcggc aaaccgctgg acgaaacacc gatcctgttg
     2461 cagccgatgg tgctcagcat ggcctggggt tcggtggtgc gtcagatcgc ggcgggactg
     2521 ggcgtcacgc tggacgagat cgtcgaggtg tactaccgcg aaccggcacc ggaggacttc
     2581 gagatcgcgt ccgggttcat cgcgaagggc acggccgccg cgctgcggtt cgaggtgcgc
     2641 gggctggtcg acgaacggcc cgcggtggtg ctcgaacacg tgacccggtt gcgcgacgac
     2701 ctcggcggtg actggccgcg accggcgcag ccgggtggct cctaccgcat cgaggtcacc
     2761 ggcgagccgt cgtatgcgct ggacctgtgc ttgagcagcc ccaacggcga ccacaatcac
     2821 gcggacctgg tcgccaccgc gatgcggatc gtcaacgcca tccccgccgt cgtcgccgcc
     2881 gagccaggca tccggaccac actcgacttg ccgctcatca ccggcaaagc gctgtacgct
     2941 ccataaaggc gttccgaaag gacattcatg cgaattcaat tccgttatct gctggcggcc
     3001 ggcgccttcg ccgcgctgct cggtgctcct gcggccgcag cggacccgac atgcatcaac
     3061 accggcgacg cgactgaatg ctcgtcgcca ggcaacgtcg agatcaacgc cactccgccg
     3121 gtttccgaat tcacgttgcc gtactgggac gagacgttcg gcggggcgta ccccggaccg
     3181 tacccggtgc cgtacgacga aggcagcggc gacatgggac gccgctaggc cagtgccatg
     3241 cggaaagcgg gtcggctagc cgcgatggcg gctctgggta tcgccgcggt tgccggcgcg
     3301 cccactgcgg cggccctgcc cgattgcggc accgtcgcgc catggacgat gcagtgcgaa
     3361 cgtggcaccc acacgtcgat caacacctcg ccgaacgtga tcgtcaacac cggcccgttc
     3421 gtgcagcagc cgtggttgta tcccggctac ccggtgttcg gaatcggcgg ctgggcggtc
     3481 ccctgaggcg taggctgggt gaaccggccc ctcgacggaa cgacggcctc gtgagattca
     3541 cgccatccgc ctaagagcca tcttgaatag gcccccgcta ggaacgtgat catcatgtcg
     3601 gcactcatac cgtcgctagc catcgtcacc gtaaccgcag gcgtcgtggc ggccgtcgct
     3661 gccgccggcc gagcagctgc ggtgcccgaa tgcaccaaca ccggtcccac caccacccag
     3721 tgcgagcgtc cgggcaacgc ccagatcaac acctcgccgg gttacaacac ctacgacaac
     3781 tacccgtggg gcttcccctg gggcagcggc atcacgatca gcctcggcgg cctcggcctc
     3841 ggcttctgac tcgtttggcg ccgaaactgt atttcagcag gccggcactc gccttccgct
     3901 gctgatccgc agtctcggcg agagccgcgg gggtttcggg taacctaact ttcctattcg
     3961 caaggccgga caatgcggag ggaaagttgg cagcgacgca gcagaccgat ctcaggctgc
     4021 ggcccagctg gtacctgctc ggtccggcct tcgtcgcggc catcgcctac gtcgaccccg
     4081 gaaacgtcgc ggcgaacgtc agcgcgggcg cgcagttcgg cttcctgctg gtctgggtga
     4141 tcctgctggc caacgtgatg gccggcctgg tgcagtacct gtcggcgaag ctcggcctgg
     4201 tgaccggccg atcgctgccg gaggcagtcg gtcggcggat gcgccgacgg acccggctcg
     4261 cctactgggt gcaggccgag ctggtggcga tggccaccga cctggccgag gtggtcggtg
     4321 gagcgctggc gctgcggctg ttgttcgact tgccgctgct cgtcggcggc gtgatcaccg
     4381 gaatcgtgtc actgctgatg ctgctgatcc aggaccggcg cggccaacgc gtgttcgaac
     4441 gcgtcatcac cgggctgctg ctcatcatcg caatcggttt cctgacaagc cttttcgtca
     4501 acccgcccgc tcccggcgac gttgtcggcg gaatgctccc ccgcttcgac ggcgccgaga
     4561 gcgtccttct ggccagcgcg atgctgggcg ccacggtgat gccgcacgcc gtgtacctgc
     4621 attccgggct ggcccgcgac cgccacggtc atccggaggg ccggcggcgg gtgcttctgc
     4681 ttcgcgtcac cagatgggac gtcgcgctgg cgatggtggt ggccggcgcg gtgaacctgg
     4741 ccatgctgct ggtggcggcc accaacctgc aggggaaccc caacaccgac tcgatcgagg
     4801 gagcgcacgc cgcggtacgc gacgcgctgg ggccgacggt cgcgctgttc ttcgcgatcg
     4861 gtctgctggc ctccggcctt gcgtcgacat cggtcggcgc atacgccggc gcgatgatca
     4921 tgcaaggcct gctgcaccgg aagatcccct tgctgctgcg gcggctgatc acgctggttc
     4981 ccgcattgac gatcctggcg atcggcatcg acccaacacg tgcgctggtg ttgtcccagg
     5041 tggtgctctc gttcggaatc ccgttcgcgc tgatcccgct ggtccggctg accagcgacg
     5101 cgtcggtgat gggctccgac accaaccacc gtgtgaccac cgccttgggc tgggtcgtcg
     5161 ccgcactcat tagtctgctc aatgtgggac tgctgtacct gaccgccacg cactgatgca
     5221 cagctacttc gcgtacggct cgaacctgtg cgtcgcgcag atggcgcggc gctgccccga
     5281 cgccaccaat cctcgccccg cgacactcgc cgaccacgac tggctgatca accagcgcgg
     5341 cgtggccacg gtcgaaccgc tcgacggaac ccacgtgcac ggcgtcgtct gggagctgtc
     5401 cgaccgcgac ctggcgacgc tggacagcgc cgaaggtgtg ccggtgcggt accgccgcga
     5461 ccggttgacc gtgcacaccg gtgacggccc cgccgacgcc tgggtgtacg tcgaccaccg
     5521 cgtcgaaacc ggcgctccgc ggccgggcta cttggaacgg atcatcgacg gagcgatcca
     5581 ccacggactg ccacagcgct ggatcgagtt cctacggcgc tgggatccgt cccagtggcc
     5641 acgtcacgta gcatctaaaa atgctgggcc acaaacactt tcggcgctac tggcacagcc
     5701 gggcgtggtc gaggaaagtc ggctgcggtc ccggttcggc ttcctggcca tccatggcgg
     5761 cggcctggag cagatgaccg acgtgatcgc cgagcgcgcc gccgacgcgg ccgaagcatc
     5821 ggtgtacgtg gtccggcatc cggatcggtg cccgcaccat ctgccgtcgg cgcgctaccg
     5881 ggcagacgag tcggcaaggc tcgcggagtt cctggatcac gtcgacgtgg tggtgtcact
     5941 gcacggctac ggccggatcg ggcgcagcac ccagatgctg gcgggtgggc gcaatcgcga
     6001 actagccgct cacctttccc gccacctcgt gctccctggg taccacctcg tcaccgatct
     6061 cgagtcgatc ccgcccgaac tgcgcggcat acatccggac aaccccgtca accgggcccg
     6121 cggcggcggt gtccacctcg agttgtcgcc gcgggtccgc gggctcagtc cgcgaagcca
     6181 gctccccggt gacgacggcc tgtcgccggt cacgtccgcc ctggtgcagg gcctcgccgc
     6241 ggccgcccgc gcctgggcgt gatgtcgccg gctccgcggc tccggggtgt gatgtcgccg
     6301 gctccgccca tccgccccac ggtcgggttc gaataccggg tgtgaaatca cgcacccccc
     6361 gcttacctgg aaccccgggc atctaccgca cgagaatcac ccatcttcgg caggcgccgg
     6421 tgaatcacta tttcgagcac cgcagctaca gctggtatgt cgacatcgac gagctgcccc
     6481 ggctgccccg gtggctgcgc gtcttcgcca gcttcgaccc gcgtgaccac ttcgctggcc
     6541 acgagggcga atccctgcgc cagcgtctcg acgcgttcct ggcccgtcac gacgagaccg
     6601 tgacaggcgg acgcatcacc gcgctgctgc agccgcgcgt gctcgggcat gcgttcaatc
     6661 cgctgaccct ctactggtgc cacgaccgcc acggtgtgct gcgccacgtc gtcgcggagg
     6721 tgcacaacac ccacggcgaa cggcacgcgt accttctccc gtcggcggcc acccctgtgc
     6781 tggccaccaa acggctgtac gcctccccgt ttcacccgct cgacggccat tacgtgatcc
     6841 gcgcaccccg gccggaccgg cgcctcgacg tcgacgtgtc gctgtaccgc ggcgacgagc
     6901 cggcgttcgt cgcgacagtg cggggcaaac gccgacccgc gacgatcacg gaagttctgc
     6961 g
//
LOCUS       DASWBF010000449         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_704788,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000449 DASWBF010000000
VERSION     DASWBF010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704788"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..>1925
                     /locus_tag="VF874_16480"
     CDS             220..>1925
                     /locus_tag="VF874_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9177598.1"
                     /translation="MTAHLFDQQASSHIDDLLEGLNPQQRQAILHEGSPLLIVAGAGS
                     GKTAVLTRRIAYLLAARDAGPGQILAITFTNKAAAEMRERVVTLVGPRARAMWVSTFH
                     SMCVRILRNQASLVKGLNSNFSIYDADDSRRLLMMIAKDMGLDTKRYSPRLLANCISN
                     LKNELIDPEQAAAEVSADSGDLNRIVADVYDEYQRRLRAANALDFDDLIGETVAVLQR
                     FPQIAQYYRRWFRHILVDEYQDTNHAQYVLVRELAGTDSADDVPPAELCVVGDADQSI
                     YAFRGATIRNIEDFERDYPNAKTILLEQNYRSTQNILTAANSVIKHNSGRREKRLWTD
                     AGEGELIVGYVAENEHDEAAFVASEIDRLADRGDITYNDVAVFYRTNNSSRSLEEVFI
                     RAGIPYKVVGGVRFYERKEIRDIVAYLRVLDNPGDAVSMRRILNTPRRGIGDRAEACV
                     AVYAENTGASFNDALQAAAEGRVPMLNSRSEKAIAAFVEMVDDLRSRLDDELGELVEA
                     VLNRTGYRAELEASNNPQDMARLDNLNELVSVAHEFSIDLANARALSEAEPEDEDVPD
                     TGV"
BASE COUNT          388 a          641 c          606 g          290 t
ORIGIN      
        1 cttctggttc gcggaccaat tccttctccg gcctcacaca agagacgagc cccggatccg
       61 gaagcggacc gcggggctct gaagtgcagc tacaccacgg gtaccgctgg ccggtacccg
      121 tagaaaaatc gccgcgtgta gagcacggtc cgagtgtgcc accaacagcc cgcgccgcgc
      181 aaaaaaatcg tcatagtggg gcggtaaatt ggggcggaca tgaccgcgca tctgttcgat
      241 cagcaagcca gctcgcacat cgacgacctg ctcgagggtc tcaaccccca acagcgtcag
      301 gcaatactgc acgagggttc gccgctgctc atcgtggccg gcgccgggtc cggcaagacc
      361 gcggtgctca cccgtcggat cgcatacctg ctcgccgcgc gcgacgccgg gccggggcag
      421 atcctggcga tcacgttcac caacaaggcc gccgccgaga tgcgcgagcg ggtagtaaca
      481 ctggtcggac cacgggcgcg cgcgatgtgg gtgtcgacgt tccactcgat gtgcgtgcgc
      541 atcctgcgca accaggcctc gctggtcaaa ggcctgaact cgaacttctc gatctacgac
      601 gccgacgact cgcggcggct gctgatgatg atcgccaagg acatgggctt ggacaccaag
      661 cggtattcgc cgcggctgct ggccaattgc atctccaacc tcaagaacga gctgatcgat
      721 cccgagcagg cggccgcaga ggtgtccgcc gactcaggtg acctcaaccg catcgtcgcg
      781 gacgtctacg acgagtacca gcgccggctg cgcgccgcca acgcactcga cttcgacgac
      841 ctgatcggcg agacagtcgc tgtgctgcaa cggtttccgc agatcgcgca gtactatcgg
      901 cggtggttcc ggcatatcct ggtcgacgag taccaggaca ccaaccacgc ccagtacgtg
      961 ctggtgcgtg agctggccgg cacagacagc gccgacgacg tgccgcccgc cgagctgtgc
     1021 gtcgtcggcg acgccgacca gtcgatctac gcgttccgcg gcgcgacgat ccgcaacatc
     1081 gaagacttcg agcgcgacta tccgaacgcc aaaacgattc tgctggaaca gaattaccgc
     1141 tccacgcaga acatcctgac ggccgcgaac tccgtcatca agcacaacag cggccgccgc
     1201 gagaagcggc tgtggaccga cgccggcgag ggggagctca tcgtcggcta tgtcgcggag
     1261 aacgagcacg acgaagccgc tttcgtcgca agcgaaatcg accgtctcgc cgaccgcggc
     1321 gacatcacct acaacgatgt cgcggtgttc taccgcacca acaactcgtc gcgctcgctg
     1381 gaagaggtgt tcatccgcgc aggcatcccg tacaaggtgg tcggcggcgt aaggttttac
     1441 gaacgcaagg agattcgcga catcgtcgcc tatctgcggg tgctcgacaa ccccggcgac
     1501 gcggtgagca tgcggcggat cctgaacaca ccaagacgcg gcatcggcga ccgcgccgag
     1561 gcgtgcgtcg cggtgtatgc cgaaaacacc ggcgccagct tcaacgacgc gctgcaggcg
     1621 gccgccgagg gtcgggtgcc gatgctgaac tcgcgctcgg agaaggccat cgccgcgttc
     1681 gtcgaaatgg tcgacgacct gcggtcccgt ctcgacgacg aactcggcga gttggtggag
     1741 gcggtgctga accgcaccgg ataccgcgcc gagcttgagg cgtccaacaa cccgcaggac
     1801 atggctcgcc tggacaacct caacgaattg gtcagcgtcg cacacgaatt cagcatcgac
     1861 ctggccaacg caagggccct gtccgaggcc gagccggagg acgaggacgt tcccgacacc
     1921 ggggt
//
LOCUS       DASWBF010000450        20885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_438835,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000450 DASWBF010000000
VERSION     DASWBF010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20885
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438835"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..720)
                     /locus_tag="VF874_16485"
     CDS             complement(289..720)
                     /locus_tag="VF874_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177599.1"
                     /translation="MLTTARSFLASQRDASKLVQEAKKFSVRLPVVGTVSVPPPDQLA
                     FYGVLGLLAAVEVIDWPVALAIGIGQAVVARHLNDQHAAATEPTAVEARAGRPAAVAA
                     PARKAAARKAAPRKAAARKSAPRKAAARKAAPGKATKSTKK"
     gene            complement(838..5169)
                     /locus_tag="VF874_16490"
     CDS             complement(838..5169)
                     /locus_tag="VF874_16490"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX9177600.1"
                     /translation="MRRSLAAVSPDVGALTRAAAGAAIEALGGPQARRASANGARRWI
                     EVGGLGGENGSAIAADVLAAVRAASGVRSAFLNRSVARVVVTVDADGPSLTEMCRIVA
                     DAERRTAAGAAYQQPRSLPGDDAVLMGRTIAAVAATMGLGLALTGTVLRLRGLPDLLA
                     VPPTLLDQVPRLRRPIERRLGAEGTDLFFALVHATTAALTLSPTAAAAETATRAMVAV
                     EAWNGRLAWRRHEPGLAEYSLPDHAAATLGDMPNPVDGPAERYADRAGLAGIGAAAVL
                     GVLSRNIGTAGAAALAATPKPLRATREAFGCALSRGLTTHHDALVLRPRALRTLDRVD
                     AIMIDPRTLYTDELMVSRVLGVHNSRRAHAWEAVRAALDEDGLAPGWHKLAAIPKGGR
                     SGQALVSPVRDPFAAAVVTEARRSHPRVVSLDDDGLRSLAQGFDHLHPLDSSIDDAVA
                     AAVAELKADGATVALLTTPDMQAAHNADITVGVLRNSRPPPWGADVFVPDLSAAWRVL
                     HALPAARSATDRGIRLSVSTSAIGALMLIPGVVGRGPESVNAGVAAGIWAGFNAAGKV
                     FRDPVPDPEPGHDWHALPVAEVQRLLPRPPDEHTEESSARWQDRQPVRGLRNLTAASW
                     NLAHDFVGEMRTNLADPITPLLATGAVASAMLGSPLDAALVGGVLLANAALSAEQQLH
                     AERILRRLLAVQDPPARRRVGPLNEGRHEMIAAKRLRPGDIIEIHADEVIPADARLIE
                     GSNVEVDESTLTGESLPVPKSTDPTPGAPLADRAGMVYAGSTLVAGTALALVTAVGAR
                     SEMRRALAMAPTKAREIGLQRQLSRITRRALPFSVGGGAVVGLLSVLRGTPLREAVSS
                     AVALTVAAVPEGLPLVATLAQLAAARRLSGESVLIRNAHSVEALARLQVVCFDKTGTL
                     SENRLQVKSVRPGKGFTAGEVLAVAASTIFVRPGHRAHHATDEAIRRAVDDSTGRHAD
                     DSPPSAITIKRDAFLPFQSGRPFAAALVGTRLTIKGAPEVLSSALADDHESLAQMITE
                     MTADGLRVLAVAHRELSPDQATAAAANSAVLEDFCNSGLTPVGLLGLADTPRASAKAV
                     LKALSDRGIGVRLITGDHPVTAAVVASELGLDVTAERVITGTEWEMMSADERAEAVSS
                     GLVFARMSPEHKIDIVQTLERIGLVTAMVGDGANDAAAIRAASVGIGVAARGSDPART
                     AADVLLLDGRIEALLDALDEGQQLWRRVQSAVSMLLGGNTGEVCFALITSLLTGRSVL
                     NARQMLLVNMLTDALPAAALAVSTQTGTAEVDRDEAAMWRAIGIRGASTTIGATLAWL
                     MASTTGTRRRAATVALIGLVSTQLAQTLADSHGRLVVLTTVGSFAVLAVVISTPVLSQ
                     VFGCTPVGPLGWGQAFLATAVAALVSALAPELLFRVSEAARRSVVDDDDTHLDEDCVD
                     LADGRRE"
     gene            5437..5853
                     /locus_tag="VF874_16495"
     CDS             5437..5853
                     /locus_tag="VF874_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX9177601.1"
                     /translation="MWSEWWSIPVSIIGALASIWLVLAVSLWLAKPDEVDSRDVVRLL
                     PDLLRLLKHLATDPQVPRGVRIQLVLLLAFLASPIDLIPDFIPVIGFVDDVILVALVL
                     RSVSRRASPAALAKHWPGTPDGLHAVLRLCGLPRTE"
     gene            complement(5911..8160)
                     /locus_tag="VF874_16500"
     CDS             complement(5911..8160)
                     /locus_tag="VF874_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX9177602.1"
                     /translation="MTENAPRVPLGAWLADLPDDRLVRLLELRPDLTQPPPGSIAALA
                     ARAQARQSVKAASGDLDFLALAVLDALLVLHADTAPVPPAKLTALLGDRVSANALSAS
                     LDALQERALVWGDANLRVVAESPAALPWHAGQATLEDSRRSAEEIAGQLDGLDDDQRQ
                     LLDRLLSGSPVGRTRDAAPGTSPDRPVQRLLAAGLLRQVDDETVILPRVAGQVLRGEE
                     PGPVSLVEPTPATSKTTARDVDAAAAGAVIDLLRETELVLETLGAAPVPELRSGGLGV
                     REAKRLTKLTGIAEQRLGLILEVSAAAGLIAAGMPRPEPLDGDGPYWAPTVQADRFND
                     LPAAQRWHLLATTWLDLPSRPGLTGRRGPDNKPIAALSDALFSTAAPLDRRLLLGVLA
                     ELPPGTGLVAADASRLLLWKRPRWSLRLQPGPVGDLIDEATTMGLVGRGALTSPARQL
                     ISGVSSDEAVAAMNRILPELIDHFLLQADLTVVVPGPLHRDLSDQLAEVATVESAGAA
                     MVYRISESSIRRALDVGRTASALHAFFKKHSKTDVPQGLTYLIDDVARRHGQLRVGIA
                     ASFVRCDDTALLAQVAAAPLAEVLQLRLLAPTVAVAQAPIADVLAALRGAGFAPAPED
                     ATGAIVDLRPRGARVTDAPSGRSYRGISVPTTQTLEAIVAMLRKVAATPPAMRVDAGT
                     AVLLLQQAVRDQSSVVMGYVDPAGVATQRVVAPISVRGGQLVAFDPASGRVRDFAVHR
                     VTSVVSVDD"
     gene            8226..8420
                     /locus_tag="VF874_16505"
     CDS             8226..8420
                     /locus_tag="VF874_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177603.1"
                     /translation="MADNKSQKSRYVDPGWPTFDGEDDHAISELAADRTGALSPFGDI
                     TFPLPADELPYTHPVTVVNK"
     gene            8478..8906
                     /gene="moaC"
                     /locus_tag="VF874_16510"
     CDS             8478..8906
                     /gene="moaC"
                     /locus_tag="VF874_16510"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX9177604.1"
                     /translation="MVDVTAKAVTTRTAVAAGALVTTPEVLALISSGGLPKGDALATA
                     RVAGIQAAKRTSELIPLCHQLALTGVDVDFEIASSAVEITATVRSTDRTGVEMEALTA
                     VSVAALTLYDMIKAVDKTARIDNVRVLRKEGGRSGSWARS"
     gene            8891..9376
                     /locus_tag="VF874_16515"
     CDS             8891..9376
                     /locus_tag="VF874_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX9177605.1"
                     /translation="MGPVLSSARVVIASTRAAAGTYEDRCGPIIVDWLGRRGFAVPDP
                     IVVADGIAVSGALRVAIAEGPDVIITSGGTGISPTDATPEATAGVLDYQIPGLADAIR
                     RAGLPKVPTAVLSRGVCGVAGRTLVINLPGSPGGVRDGLAVLGDVLDHALDQLRGKDH
                     T"
     gene            9373..9798
                     /locus_tag="VF874_16520"
     CDS             9373..9798
                     /locus_tag="VF874_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX9177606.1"
                     /translation="MTRVLRAEITEQPVVLAELEALVADEAAGAVVSFAGVIRDHDGG
                     RQVIRLEYSAHPSAQDTLAEVCAEIAREADGVRALAATHRIGTLAIGDAALVAAVAAE
                     HRGAAFETCAKLVDTVKARLPVWKHQFFADGTDEWVGSA"
     gene            complement(9810..10991)
                     /locus_tag="VF874_16525"
     CDS             complement(9810..10991)
                     /locus_tag="VF874_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX9177607.1"
                     /translation="MNGRHRKPTQSNINVAKVAFTGAVIGGSGLALSAQAAAATDGEW
                     DQVARCESGGNWAINTGNGYQGGLQFSPGTWSSAGGSTYAPAAHLASRDEQIAVAERV
                     LARQGRGAWPVCGTGLSSATPRTVPVVNDVRTLEAPAALDAPLPPEGEPPAPEALAPE
                     APPAPEALPPAPEELPPAPEVLPPAPEALPPAPEELPPAPEALPPAPEAPPAEPVIET
                     VAAPEVPDDVVLPAADQLSAPTEDVIVGAALSEPAGEKFGAAPEQTAVIQQTSREVPL
                     APIDTPPAPPLPDIPAPVYDAANQVMTDGTIPAVPQEVPHLASPENLPPGTTTDAPQQ
                     GPRATYLKELWEAVRNQDVTLNDAILGLAQRPMDANATPPPGLAAGPQAPLPEAPIPP
                     V"
     regulatory      complement(11001..11209)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(11415..11684)
                     /locus_tag="VF874_16530"
     CDS             complement(11415..11684)
                     /locus_tag="VF874_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9177608.1"
                     /translation="MPELSTKLLVTVRYFGAARAAAATESETLTVEPDMSIADLTNQL
                     GERNPTLAKVLARCSYLCDGVAARDTAAQVRTGQIIDVLPPFAGG"
     gene            complement(11685..12749)
                     /gene="moaA"
                     /locus_tag="VF874_16535"
     CDS             complement(11685..12749)
                     /gene="moaA"
                     /locus_tag="VF874_16535"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX9177609.1"
                     /translation="MTVTALGLPSVLSKGVPTDESAPTGGPLIDSFGRVATDLRVSLT
                     DRCNLRCTYCMPAEGLDWLPTEHLLRPDELIRLLRLAVSRLSITSVRFTGGEPLLSKN
                     LEEIVAATARLRPRPEITLTTNGVGLARRADRLAAAGLDRINVSLDTVDASHFAAITR
                     RNRLPDVLDGLKAAQAAGLDPIKVNAVLDPATGRDDAVALLQFCLDHGYQLRIIEQMP
                     LDAQHGWKRGEVLTADDVLAALRRHFMLQPDPVLRGSAPAELWLVDGGPATVGVIASV
                     THAFCSACDRTRLTADGQVRNCLFATAETDLRGLLRSGADDAAIEAAWRAAMWAKAAG
                     HGINDPSFVQPARPMSAIGG"
     gene            complement(12746..13138)
                     /locus_tag="VF874_16540"
     CDS             complement(12746..13138)
                     /locus_tag="VF874_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEX9177610.1"
                     /translation="MRLILNIIWLVFGGLWLALGYLLAALVCFILIITIPFGIASLRI
                     ASYALWPFGRTIVDKPEAGAGALVGNVIWIVLFGWWLALEHIVTAIAMAITIIGIPLA
                     LANLKLIPVSLLPLGKEIVPVDDTRTPV"
     gene            13278..13691
                     /locus_tag="VF874_16545"
     CDS             13278..13691
                     /locus_tag="VF874_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9177611.1"
                     /translation="MPTGRVKWYDPEKGFGFLSQEDGEDVYIRSSALPAGVEALKSGQ
                     RVEFGVASGRRGPQALSLKLIDPPPSPLRTGRRETGPAEHKHTPDELHGMVEDMITLL
                     EGAVQPELRKGRYPDRKTARRVSEVVKAVARELDA"
     gene            13781..14791
                     /locus_tag="VF874_16550"
     CDS             13781..14791
                     /locus_tag="VF874_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9177612.1"
                     /translation="MAAYVASGGEFERDTDYITTRITPDGRDGYPVEPGRYRLIVARA
                     CPWANRAIIVRRLLGLEPVLSIGFCGPTHDERSWTFDLDPGGVDPVLKIPRLQDAYFA
                     RFPNYPKGITVPAIVDVPTGQVVTNDFAQITLDLSTEWRQYHRDGAPDLYPEPLRAEI
                     DEVTQRIYTEINNGVYRCGFAGSQRAYDRAYDRLFTALDWVSERLSGQRFLVGDTITE
                     ADVRLFTTLARFDPVYHGHFKTNRQKLSEMPVLWAYARDLFQTPGFGDTADFVQIKQH
                     YYIVHKDINPTRIVPKGPDLSNWLTPHHREELGGRSFGDGTPPGPPIEGERVVPGHSP
                     LA"
     gene            complement(14773..15276)
                     /locus_tag="VF874_16555"
     CDS             complement(14773..15276)
                     /locus_tag="VF874_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2771 domain-containing protein"
                     /protein_id="HEX9177613.1"
                     /translation="MTRVLTALAAVVVLVCGVVAVGIWLLVRDQPPQRPEISAYSRGQ
                     LTRVGPYRYCNVLNLEDCENYETEGELGVDERDPVQLSVPTSIGRTPWLLRAFYNDPR
                     DSTLDVFRPNSRLAVTIPTVGSNGGKLNRVVVQLLTLVQDEKGDLFPMPHAEWSVRTE
                     WVQASGE"
     gene            complement(15273..16874)
                     /locus_tag="VF874_16560"
     CDS             complement(15273..16874)
                     /locus_tag="VF874_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9177614.1"
                     /translation="MANYPTDDAPYRRPRREPTASANRYLPPLDEPARHNAPPPRSGP
                     SAPGGQKVTVTRAAAMRSREMGVRMYGLFHRAATADGADKSGLTALTYPVMANFAVDA
                     AMAVALANTLFFAAASAESKSRVALYLLITIAPFAVVAPLIGPALDRLQHGRRVALAM
                     SFVLRTALAIVMIANYDPATGSFPPWVLYPCALLMMVLSKSFSVLRSAVTPRVMPPTI
                     DLVRVNSRLTVFGLIGGTIIGGGIAAGFEYTFTKLFHLPGALFVIVVVAVVGAYLSMQ
                     IPRWVEVTTGEVPTTLTYHGRSGPLRRDSHGNTASRPSRSGRPRQPLGRNIITSLWGN
                     VTIKVMVGFLFLYPAFVAKQTGQNGWHQVALLGMIGAAATVGNFAGNGLAARLKLGHP
                     SLLVVRCATMVTAAALAAAFFGDLIVMAIAALITSGCSAVGKASLDACLQDDLPEQSR
                     ASAFGRSESLLQLAWVAGGATGVLVYTDLRVGFTAISSFLILGLAQTIVSYRGDSLIP
                     GFGGNRPVVAEQEGRAPDGAMVTPE"
     gene            17056..17793
                     /locus_tag="VF874_16565"
     CDS             17056..17793
                     /locus_tag="VF874_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HEX9177615.1"
                     /translation="MDDVTVGLDGAVDQARAAIAEYSSEDTVGEYLGVNYDDAHSATH
                     RFLANLPGYCGWQWAVVAAAHPGADHATISEVVLVPGPTALLAPRWVPWEARVRPGDL
                     GPGDLLAPPRDDLRLVPGYTATGDPAVDDIAVEFGLGRRQVLSGSGRAGAAQRWRDGE
                     YGPHSAMARSVRRVCRDCAFYLPLAGLLGRMFGICANEMSADGHVVDAEYGCGAHSDT
                     PAPAGNGSPRYDPFDDGVLDLLENQEN"
     gene            complement(17790..18245)
                     /locus_tag="VF874_16570"
     CDS             complement(17790..18245)
                     /locus_tag="VF874_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9177616.1"
                     /translation="MPAPLLQAQIDVDAPVDKVWQLITDLRRMPEWSPQCRLMRPLGS
                     LRQGTRTINLNRRNRLFWPTTGTITEIVPQRKFAFRVNTNNTVWSYELEPTATGTRVV
                     ESRHAENGVKPASNFLVNLLLGGVPNFEQELVDGMNASLARIKAVAERR"
     gene            complement(18262..18525)
                     /locus_tag="VF874_16575"
     CDS             complement(18262..18525)
                     /locus_tag="VF874_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HEX9177617.1"
                     /translation="MTDEPADQSEVQPPSLPDALLDPRPVIAVCTLAWIVAVIVAFTV
                     PSLETWRPVAVAGLGLAGFGTSVFLWQRRAARRGARGAQTGLR"
     gene            18550..19365
                     /locus_tag="VF874_16580"
     CDS             18550..19365
                     /locus_tag="VF874_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9177618.1"
                     /translation="MAHLVDVANPADARLDDFRNLNSIDRRPDLPSGKGLVIGEGVLV
                     VQRMLASRFSPFAVMGNDRRLGDLREDLAAVDIPYYRVDADVMAQVVGFHLNRGVLAA
                     ARRPPMLTVRDVIKDARTLAVLEGVNDHENLGSIFRNAAGLGVDAVVFGPGCADPLYR
                     RAVRVSMGHALLVPCASVETWPADLNLLWENGFQLLAMTPDPDATTLADAMAHVGDGK
                     VAILVGAEGPGLTHKAMRASDMRVRIPMSRGTDSLNVATAAALAFYERVRLAR"
     gene            19428..19694
                     /locus_tag="VF874_16585"
     CDS             19428..19694
                     /locus_tag="VF874_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2537 domain-containing protein"
                     /protein_id="HEX9177619.1"
                     /translation="MTDYSTPWGTGLTVAAFVAAVTAAAIVVLSLGVSRVHPLLAVAL
                     NLIAVGGLAPTLWGWRAVPVLRWFVLGGGVGIVGAWLALLALAV"
     gene            complement(19775..20533)
                     /gene="sepH"
                     /locus_tag="VF874_16590"
     CDS             complement(19775..20533)
                     /gene="sepH"
                     /locus_tag="VF874_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HEX9177620.1"
                     /translation="MQELTVVSLDVDGKHIICESTRPGEKFLLRADDRLRAAVRGEGT
                     RSSQTEIDIEVTNVLSPKEIQSRIRAGASVEHVATSAGVDVSRVERFAHPVLLERSRA
                     AELATASHPMLADGPSVQTLLETVATALVGRGLDHDATSWDAWKNADGRWTVQLTWLA
                     GRSQNVAHFRFTPGAHGGTAVALDDPAKELIDPDFDRPLRPVAPVAQLDFDDAAPQEL
                     AVEEPVTPPRARRSKPAVPTWEDVLLDVRSGGHH"
     gene            complement(20644..20826)
                     /locus_tag="VF874_16595"
                     /pseudo
     CDS             complement(20644..20826)
                     /locus_tag="VF874_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
BASE COUNT         3339 a         7174 c         7079 g         3293 t
ORIGIN      
        1 atcacacgaa atcaccatat ctattattga gcatcgggac cgcggccaca cacacgacac
       61 accggaaatc cgcagatcac aacaccgaat taccgaccgg acacaggccc catgtcagga
      121 gtcctggacc ccggttcgca caaagtcggg tacgggactt cgtccccctg ctcgttgagc
      181 gacgagcttc cgacgagttg tgtcgacgca cggaaatgct cgacatccct gcctaaaggg
      241 agctaagggc gttcacgccc cgcgtgctgc gggcccatca gcggccactt atttcttggt
      301 cgacttggtg gccttgccgg gcgccgcctt tcgcgcagcg gccttgcgcg gagcggactt
      361 tcgcgcagcg gccttgcggg gagccgcctt tcgcgcagcg gccttgcggg ccggcgcagc
      421 gactgctgcg ggcctgcccg cgcgggcttc gaccgcggtg ggctcggtgg cggccgcgtg
      481 ctggtcgttg aggtggcggg cgaccacggc ttggccaatg ccgatcgcca gggccaccgg
      541 ccagtcgatg acctccacgg cggcgagcag gccgaggacg ccgtaaaacg cgagttgatc
      601 aggcggtggt accgacaccg tgcccaccac cggaaggcgg accgagaact tttttgcttc
      661 ctggaccagc ttggaggcgt cccgctgcga ggcaagaaat gatcgagccg ttgtcaacat
      721 ttcgtgtcct gttcaattct gtgaccctca tttgtcacac cggtcggatt gtttggaacc
      781 gtgcccgaat tcctgcgcag cgtcggtcat gacccgctga ttatggctgg tgttcgtcta
      841 ttcacgccgc ccatccgcga ggtctacgca gtcctcatcc aggtgggtat cgtcgtcgtc
      901 gacgactgac cgccgcgccg cttccgacac acggaacaac agctccggtg cgagcgccga
      961 caccagagcg gccaccgcag tcgccaggaa cgcctgaccc cagcccagcg gccccacggg
     1021 cgtgcacccg aacacctggc tcaacaccgg cgtgctgatc accaccgcca acacggcgaa
     1081 cgacccgaca gtcgtcaaca ccaccagccg accgtgagaa tcggcgaggg tctgtgccag
     1141 ctgtgtgctg acaagcccga tcaacgccac tgtcgcggcc cggcgtcgag tacctgtcgt
     1201 gctggccatc agccatgcca acgtcgcacc gatcgtcgtc gacgcgccgc gaattccgat
     1261 cgcgcgccac atcgccgcct cgtcccgatc gacctcggcg gtaccggtct gcgtgctgac
     1321 ggccaaggcg gcggccggca acgcgtcggt cagcatattg acaagcagca tctggcgcgc
     1381 gttgagcacc gaccgtccgg tcagcaggct ggtgatcagc gcgaaacaca cctcgccggt
     1441 gttgccgcca aggagcatcg acaccgccga ctgcacccgg cgccacagct gctggccctc
     1501 gtcgagagcg tcgagtaagg cttcgatacg tccgtcgagc aggagcacgt cggccgcagt
     1561 gcgggccggg tcgctgccgc gtgccgccac gccgatgccg acgctggcgg cccggatcgc
     1621 cgcggcgtcg ttcgcgccgt cgccgaccat ggccgtgacg aggccgatcc gctccagcgt
     1681 ctggacgatg tcgatcttgt gttcgggtga catccgcgcg aagaccaacc ccgacgacac
     1741 cgcctcggcc cgttcatcgg ccgacatcat ctcccactcg gtgccggtga tcacccgctc
     1801 ggcggtcacg tccagaccca attcactggc gacgacggcc gcggtgacgg ggtgatcgcc
     1861 ggtgatcaac cggacgccga taccgcggtc gctgagtgcc ttcaacaccg ccttggcgct
     1921 cgcgcggggg gtatccgcca aacctaggag cccaaccggg gtgagcccgg aattgcagaa
     1981 gtcttcaagt acagccgaat tggccgcggc ggccgtggcc tggtcggggc tgagctctct
     2041 gtgggccacg gccaggaccc ggagtccatc ggcggtcatc tcggtaatca tctgcgccag
     2101 cgactcgtgg tcatcggcca gtgccgacga gaggacctca ggtgcgcctt tgatggttag
     2161 tcgagtgccg accagcgccg ccgcgaacgg gcgaccggac tgaaacggca gaaatgcgtc
     2221 gcgtttgatc gtgatggccg atggcggcga gtcgtcggcg tgtcgaccag tcgagtcgtc
     2281 gacagccctg cgtatcgcct cgtcggtggc atggtgagct cggtgcccgg gcctgacgaa
     2341 gatcgtgctg gcggctaccg cgagcacctc gccggcggtg aaccccttcc ccgggcgcac
     2401 cgacttcacc tgcagacggt tttcgctgag tgtgccggtc ttgtcgaagc acaccacctg
     2461 gagccgcgcg agcgcctcga ccgaatgagc gttgcggatc agcacggact cgccgctgag
     2521 gcgccgcgca gcagcgagct gcgcgagggt cgccaccaag ggcaatcctt ccggcacggc
     2581 ggccacggtc aacgcgaccg cactgctcac ggcctcgcgc agcggcgtgc cgcgcaacac
     2641 gctgagcagc ccgaccacgg cgccgccgcc cacgctgaag ggcaacgccc gtcgggtgat
     2701 gcggctcagc tgtcgctgca agccgatttc tcgtgccttg gtcggggcca tcgccaacgc
     2761 ccggcgcatt tccgagcgtg ccccgactgc ggtgaccaag gccagagcgg tgcccgcaac
     2821 cagtgtgctg cccgcgtaga ccatgccggc ccgatcggcc aacggtgcac caggcgtggg
     2881 atcggtcgat tttggcaccg gcagcgattc gccggtcaac gtcgattcgt cgacttcgac
     2941 gttcgaccct tcgatcaacc gggcgtcggc gggaatcacc tcatcggcat ggatctcgat
     3001 gatgtcgccc ggacgcagcc gcttggccgc gatcatttcg tgacgcccct cattgagcgg
     3061 ccccacgcgc cgtcgcgccg gtgggtcctg cacggcaagc agccgacgca agatgcgctc
     3121 cgcatgtagc tgctgttcgg cggacaacgc ggcgttggcc agcagcacac cgcccacgag
     3181 ggccgcgtcg agcggcgagc cgagcatcgc gctggccacc gcaccggtcg ccagcagcgg
     3241 cgtgatcgga tccgccaggt tggtacgcat ctcacctacg aaatcgtggg cgaggttcca
     3301 ggaggcggcg gtcagattcc gcagaccgcg aaccggttgc cggtcttgcc accgcgcgga
     3361 tgactcctcg gtgtgctcgt cgggaggccg gggcaacagc cgctgcacct ctgcgaccgg
     3421 cagggcatgc cagtcgtggc cgggttctgg gtccggcact gggtcgcgga agacctttcc
     3481 ggccgcgttg aatccagccc atataccggc ggccaccccc gcgttgaccg actccggtcc
     3541 gcgcccgacc acgccgggaa tcaacatcag cgcgccgatc gccgaggtgg agaccgacag
     3601 ccggatgccc cggtcggtgg cggagcgcgc ggcgggcaaa gcgtgcagta cgcgccaggc
     3661 agcgctcagg tccgggacga acacgtcggc accccacggg ggaggccgac tgttccgcag
     3721 cacgccgacc gtgatgtcgg cgttgtgcgc cgcctgcatg tccggggtgg tgagcaatgc
     3781 cacggtcgca ccatcggcct ttagttcggc gacggctgcc gccaccgcgt cgtcgatcga
     3841 gctgtccaac ggatgcagat gatcgaatcc ttgtgccaga gaacgcaatc cgtcgtcatc
     3901 gagcgagaca acgcgcgggt gcgaccgccg cgcctcggtt accaccgccg cggcgaaggg
     3961 atcgcgaacc gggctcacca gcgcctgccc ggatctgccg cctttgggga tggccgccag
     4021 cttgtgccaa cccggagcca gcccgtcctc gtcgagtgcc gcgcggaccg cctcccaggc
     4081 gtgcgcgcgg cgcgaattat gtacgcccag aacacggctg accatcagtt cgtcggtgta
     4141 gagggttcgc gggtcaatca tgatcgcatc gacccggtcg agcgtccgca gggctcgagg
     4201 acgcaacacc agggcgtcgt gatgggtggt cagaccccgg ctcagtgcgc acccgaaagc
     4261 ttcccgggtg gcgcgcagcg gcttcggggt ggcggccaac gccgccgcac cggccgtgcc
     4321 gatgttgcgc gacaggacgc cgagcacagc ggcggcgccg atgcccgcca agcccgctcg
     4381 atcggcataa cgctctgccg gaccgtcgac cgggttcggc atgtcgccga gggtcgcggc
     4441 cgcgtggtcg ggcagcgaat actcagccag ccctggttcg tgccgacgcc atgccagacg
     4501 accgttccaa gcctcgaccg cgaccatcgc ccgcgtggcg gtttcggcag ccgccgccgt
     4561 tggcgacagt gtcaacgcgg ccgtcgtcgc gtggaccaac gcgaagaaca gatccgtgcc
     4621 ctcagctccg aggcgccgtt caatggggcg acgcagcctt gggacctgat ccaggagcgt
     4681 ggggggcacc gccagcaggt ccggcaatcc acgcaaccgc agcaccgtgc cggtcagtgc
     4741 gagtcccaaa cccatagtgg ctgccacggc ggcgatcgtt cggcccatca ggacggcatc
     4801 gtcgccgggc aggctcctcg gctgctggta ggcagcgcca gcagccgttc ggcgttcggc
     4861 gtcggcaacg atccggcaca tttcggtcaa cgaggggcca tcggcatcca cggtcaccac
     4921 cacccgggcc acggaccggt tcaggaacgc cgaccggacc ccgctcgcgg cgcgtactgc
     4981 cgccaacaca tcggcggcga tcgcggagcc gttttcgccg ccgagccctc ccacctcgat
     5041 ccaccggcgg gcgccgttgg cgctggcgcg ccgtgcctgc ggcccgccga gtgcctcgat
     5101 ggccgcaccc gcggcggccc gagtcagcgc tcccacgtcc ggggataccg cggccagtga
     5161 ccttcgcatc cccgcgaagc tgccgcccaa cagagccgtg ctgcacgcgg tggcggcgac
     5221 gcccgcggtc gccgcaatcc gggtgccggc tacggcggcg cgcaaggggg ccgtgatcag
     5281 cccagaaccc ggcagagcgc gccgcgtgat cacttcgccg aacataccga gtgcggcggg
     5341 gacttgcccg cgccaaagca gaagccaggt tgactgtagg tgaacatccg gctaaccaca
     5401 gcggcggaga agatccgttg ccgccaagat cggagggtgt ggtccgaatg gtggtccatc
     5461 ccggtcagca tcatcggcgc ccttgcgtcg atttggctgg tgcttgccgt ctcgctgtgg
     5521 ctggccaagc cagatgaggt ggactcgcga gatgtggtcc ggctgctgcc cgacctgttg
     5581 cggctactca agcacctcgc cactgatcca caggtaccgc gcggagtccg gattcagctg
     5641 gttctgctgc tcgccttcct cgcatcgccg atcgatctca tcccggactt catcccggtg
     5701 atcggctttg tcgacgacgt catcctcgtg gcgctggtgc tgcgctcggt ctcccgacgc
     5761 gcaagtcccg ccgcgcttgc caaacactgg cccggcacac cagacggcct gcacgcagtg
     5821 cttcgactgt gcggactccc gcgaacggaa tgaaccgacg cgggtgtctg gcccggcgtg
     5881 accgtctccg cgcccgcgat catctgcgta tcaatcgtcg accgacacca ccgacgtgac
     5941 gcgatgcacc gcgaagtccc gcacccggcc cgacgccggg tcgaacgcca cgagttgccc
     6001 gccgcgcacg ctgatcgggg ccacgacgcg ctgggtggcc acgcctgccg ggtcgacgta
     6061 gcccatcacc accgagctct ggtcccgcac ggcctgctgc aacagcagca ccgcggtgcc
     6121 ggcgtctacg cgcatcgccg gcggcgtcgc cgcaaccttg cgcagcatcg cgacgatcgc
     6181 ttcgagtgtc tgagtggtcg ggacgctgat accgcgatag gaccgtccgg acggtgcgtc
     6241 ggtcacccgt gctccgcgcg gccggaggtc gacgatcgcg ccggtcgcat cctcgggcgc
     6301 cggggcgaag ccggcaccgc gcagcgcggc gaggacgtcg gcgatcggcg cctgcgccac
     6361 cgcgaccgtc ggggccagca gccgcagctg caacacttcc gccaacggcg ccgcagccac
     6421 ctgtgccagc aacgcggtgt cgtcacaacg cacgaacgac gcggcgatgc ctacgcgcag
     6481 ctggccgtga cgtcgggcaa catcgtcgat gagataggta agaccttgcg gaacatccgt
     6541 tttcgaatgc tttttgaaga atgcgtgcag cgcgctcgcc gtgcggccga cgtcgagcgc
     6601 gcgccggatc gacgattcac tgatccggta gaccatcgcg gcgccggcgg actccacagt
     6661 ggccacctcg gcgagctgat cggacaagtc gcggtgcagt ggcccgggta ccaccaccgt
     6721 caggtcggcc tgcaagagga agtggtcgat caactcgggc aggatccggt tcatcgcagc
     6781 gacggcctcg tcactggaga cgccggagat cagctgccgc gcaggcgaag tcagtgcacc
     6841 gcgccccacc aggcccatgg ttgtcgcctc gtcgatcagg tcgccgactg gccccggctg
     6901 cagtcgcagc gaccagcgcg gccgtttcca caacagcaac cgcgatgcgt ctgcggcgac
     6961 aagccctgtg ccaggcggta attcggcgag cacaccgagc aggagccgac gatccagcgg
     7021 tgcggcggtg gagaacagcg catccgacag cgccgcgatc ggtttgttgt ccggtccgcg
     7081 tcgccccgtg agccccggcc gcgacggcag gtccaaccac gtcgtcgcca acagatgcca
     7141 gcgctgggcg gccggtaggt cgttgaagcg gtcggcctgc accgtcgggg cccaatacgg
     7201 cccgtcgccg tcgagcggtt cgggccgtgg catccccgca gcgatcaggc cagccgcggc
     7261 gctgacctcc aggatcaagc ccagccgctg ctcggcgatg ccggtcaact tcgtcagccg
     7321 ctttgcttcc cgcaccccga gccccccgct gcgcagttcg ggaaccggtg cggcgccgag
     7381 ggtttcgagc acgagctctg tttcgcgcag caggtcgatc acggcgcccg cggcggccgc
     7441 gtcgacgtcg cgggccgtcg tcttcgacgt ggctggtgtc ggctcgacca gactgaccgg
     7501 acccggctcc tcgccgcgca acacctggcc cgccacgcgc ggcaggatca cggtctcgtc
     7561 gtcgacctga cgcagcaacc cggccgcgag cagccgctgc accggccggt cgggcgacgt
     7621 gccgggggcg gcgtcgcggg tccggccgac cggcgatccg gacagcagac ggtccaacag
     7681 ttgacgttgg tcgtcgtcga gcccgtcgag ctgtccggcg atttcctcgg cgcttcggcg
     7741 gctgtcctcg agcgtggcct ggccggcatg ccacggcagc gcggcgggcg actcggcgac
     7801 cacccgaagg ttcgcatcgc cccacaccag ggcccgctcc tgcagcgcat ctaatgaggc
     7861 agacagcgca ttcgccgaca cgcggtcacc gagaagcgcc gtcagcttgg cgggtggcac
     7921 aggggcggtg tctgcgtgca gcaccagcag cgcgtccagc acggcgagcg ccagaaagtc
     7981 gaggtcgccg ctggcggctt tcaccgactg acgtgcctgc gcccgcgccg ccagcgctgc
     8041 gatgctgccc ggcgggggtt gcgtcaggtc gggtcgcagc tccagtagcc ggaccagccg
     8101 gtcgtcgggc aggtcggcca gccaggcacc cagcgggacc cgcggggcgt tctcggtcat
     8161 tgctgaccag cgtaaaggag ccagtcagtg tcgcgacggg ccgtcacgcc tgtcagaatg
     8221 aagccgtggc tgacaacaaa tcgcagaaga gtcgatacgt cgaccccggc tggccaacgt
     8281 tcgacggcga ggatgaccac gccatcagcg agctcgccgc cgaccgcacc ggcgcattgt
     8341 cgccgttcgg ggacatcacg ttcccgctgc ccgccgacga gctgccgtac acgcacccgg
     8401 tgacggtggt caacaagtag atcgtgacct ctgacgccgg ccggctttcc cacctcgacg
     8461 aacgcggcgc ggcccacatg gtggacgtca ccgcgaaggc ggtcactaca cgcacggcgg
     8521 tggcggccgg ggcgttggtg acgacgccgg aggtgctggc tttgatctct tctggcggcc
     8581 tgcccaaggg cgacgcgcta gcgaccgcgc gggtggccgg catccaggcc gccaagcgca
     8641 ccagcgagct gattccgctg tgtcatcaac tcgcgctcac cggcgtcgac gtcgacttcg
     8701 aaatcgcttc gtccgcagtc gagatcaccg ccacagtccg cagcaccgac cgcaccggcg
     8761 tcgagatgga ggcgctcacc gcggtgagcg tggcagcgct gaccctctac gacatgatca
     8821 aggccgtcga caagactgcg cgcatcgaca acgtccgcgt gctccgcaag gagggcggcc
     8881 gaagcggcag ctgggcccgg tcttgagctc ggctcgcgtc gtgatcgcat ccactcgcgc
     8941 cgcagcgggt acgtacgaag accgttgcgg cccaatcatt gtcgactggc tgggtcggcg
     9001 cggattcgcc gttcctgacc cgatagtggt cgccgacggc atcgccgtga gtggggcgtt
     9061 gcgggtggcg atcgccgagg gccccgatgt gattatcacg tccggcggta ccggcatctc
     9121 gccgacggat gccacgcccg aagcgactgc gggagtgctg gactatcaga ttcccgggct
     9181 cgccgatgcg atccggcggg caggtctgcc gaaggttccg acagcggtgc tgtcccgggg
     9241 ggtctgcggg gtcgccggcc gaacattggt gatcaacctg cccggctcac ccggcggagt
     9301 gcgagacggt ctggccgtat tgggcgacgt gctcgaccac gcgctggacc agctgcgcgg
     9361 aaaggaccac acatgacgcg ggttcttcgt gccgagatca cggaacaacc cgtcgtgctc
     9421 gccgagctcg aagcgttggt agccgacgag gccgcggggg ccgtggtgag cttcgccggg
     9481 gtgatccgcg accatgacgg cgggcgtcag gtgattcgcc tggagtactc ggcgcatcct
     9541 tccgcccagg acacgctggc cgaggtatgc gcggagatcg ctcgcgaagc cgacggtgta
     9601 cgtgcgctgg ccgctaccca ccgcatcggg actctggcca tcggcgatgc ggcgctggtg
     9661 gccgcggtgg ccgccgagca tcgcggcgcg gcgttcgaga cgtgcgcgaa gctggtcgac
     9721 accgtcaagg cgcggctacc ggtgtggaag caccagttct tcgccgacgg gaccgacgaa
     9781 tgggtgggat cggcctgagc cgctcctgac tacacgggcg gaatcggcgc ctcaggcagt
     9841 ggcgcttgcg gacccgcagc cagtcccgga ggtggcgtcg cgttggcgtc catcggccgc
     9901 tgcgcaagcc ccaggatcgc gtcgttgagc gtcacgtcct ggttgcggac tgcctcccac
     9961 aactctttga ggtacgtcgc ccgtgggccc tgctgcggcg catccgtggt ggtgcccggc
    10021 ggcaggttct ctgggctcgc caggtgcgga acctcctgcg gcacagcggg aatcgtgccg
    10081 tcggtcatga cctggttggc cgcgtcgtac accggagccg ggatgtcagg cagcggcgga
    10141 gcgggcggcg tatcgatggg agccagcgga acctcacgcg aggtctgctg gatcacggcg
    10201 gtctgctcgg gcgcagcacc gaacttttcc cccgcaggtt cgctgagcgc cgcgccgacg
    10261 atcacgtctt cggtcggtgc ggacagctgg tccgccgcgg gcagcacgac gtcgtcgggt
    10321 acctccggcg cggcgactgt ctcgatgacg ggctcggccg gcggagcttc gggcgcgggc
    10381 ggcagagctt cgggcgccgg gggcaattct tcgggcgcgg gcgggagagc ttcgggcgcg
    10441 ggcgggagaa cttcgggcgc cggcggcaac tcttcaggcg ctggcgggag agcttctggc
    10501 gcaggcggag cctcgggcgc cagagcctca ggtgcgggcg gctcgccttc cggcggcagc
    10561 ggggcgtcca gcgcagccgg ggcctcgagg gtccgcacgt cgttgactac tggaacggtg
    10621 cggggtgtgg cggaggacag cccggtgcca cacacgggcc acgcgccgcg accctgtcgc
    10681 gccagcacgc gctcggcgac tgcgatctgc tcgtcgcgcg aagccagatg cgccgcgggg
    10741 gcgtacgtgc tacctccggc cgaactccag gtgccggggg agaactgcaa cccgccttgg
    10801 tagccgttgc cggtgttgat ggcccagttg ccgccggatt cgcagcgggc gacttggtcc
    10861 cattcgccat cggtggctgc tgctgcctga gcgctcaggg ccaggccgct gccgccgatg
    10921 accgcgccag tgaaggcgac tttcgcgaca ttgatgttgg attgggtggg cttgcgatgc
    10981 cgtccattca tgcgccgaaa cattcctctc tgcacgcgcc tgcggagtca gctgtcgggt
    11041 tcgggcaaga gaggtcgccc ggccggtgtc gcgttgccgc gccggcttca ccccaaggag
    11101 ccgcacgcgg ctcctggtcc gatgtcggtg gaccggatgg gtcccccgcc tccatccacg
    11161 gtgtcgtcgt gtccttgtcc tcacggatgg agctcggcgc gaaaggaaag gaaggccaac
    11221 cgcattgacg tgggcgggct tgcggaagac cgtaacgcct tgcgcgattt ccgtcacctt
    11281 tgggctttcc tggcgtttcg gggcgcggcc agggggaaaa ccccaagagg ggccggtgtt
    11341 cccgcaggtt gatcgtggcc gataccggcc cgagtcccgg ctgtgatcaa accgttatgt
    11401 gacgcaaatc acgttcatcc gcccgcaaag ggcggcagca cgtcgataat ctgcccggtt
    11461 cgcacctgcg cggccgtgtc ccgggcggcg acgccgtcgc acaggtacga acaccgcgcc
    11521 agcaccttcg ccagcgtcgg attccgttca cccagttggt tcgtcaagtc agcgatgctc
    11581 atgtcgggtt caaccgtcaa agtctcggat tcggtggccg cggccgctcg ggcggcgccg
    11641 aagtaccgca cggtcaccag gagtttcgtc gacagttcgg gcattcagcc gccgatcgcg
    11701 ctcatcggcc gggccggctg gacaaagctc gggtcgttga tcccgtgtcc ggcggccttc
    11761 gcccacatcg cggcccgcca cgccgcctcg atggccgcgt cgtcggcgcc gctgcgcagc
    11821 agcccacgca ggtccgtctc ggccgtcgcg aacagacagt tacgaacctg gccgtcggcg
    11881 gtcaaccggg tgcggtcgca ggccgagcag aacgcgtgtg tcacggacgc gatcacgccg
    11941 accgtcgcag gaccgccgtc caccagccac agctcggccg gcgccgagcc acgcagcaca
    12001 ggatcgggct gcaacatgaa atggcgccgc aatgcggcca ggacgtcgtc ggcggtcagc
    12061 acctcaccac gtttccagcc gtgctgggcg tccaacggca tctgctcgat gattcgcagc
    12121 tgataaccgt ggtcgaggca gaactgcagc agcgccacgg cgtcgtcgcg gcccgtggcc
    12181 gggtcgagca cagcgttgac cttgatcgga tcgagcccgg ccgcctgggc cgccttgagt
    12241 ccgtcgagca cgtcgggcag ccggttccga cgcgtgatcg cggcgaagtg ggacgcgtcg
    12301 acggtgtcga gcgacacgtt gatcctgtcg agtcccgcag cggccaaccg gtccgcgcgg
    12361 cgggccagcc cgacgccgtt ggtggtgagc gtgatctccg gccggggccg taaccgcgcc
    12421 gtcgccgcga cgatctcctc gaggttcttc gacagcagcg gctcgccgcc ggtgaaccgc
    12481 acgctggtga tgctcaaccg gctgacggcc agacgcagaa ggcggatcaa ttcgtcaggc
    12541 cgcagtaggt gctcggtggg tagccagtcg aggccttcgg ccggcatgca gtaggtacat
    12601 ctcaggttgc agcggtccgt cagcgagacc cgaagatcgg tggccactcg gccgaagctg
    12661 tcgatgagcg gcccgccggt gggcgcgctc tcgtccgtcg gcaccccttt ggaaagcacc
    12721 gacggcaagc ccagcgctgt gaccgtcata ccggggtcct ggtgtcatcc accggcacga
    12781 tctccttgcc tagcgggaga agcgacaccg gaatcagctt gaggttggcc agcgccagcg
    12841 ggatgccgat gatcgtgatc gccatcgcga tcgctgtgac gatgtgttcg agcgccagcc
    12901 accatccgaa cagcactatc cagatgacgt tgccgaccag cgcgccggcg cccgcctcgg
    12961 gcttgtcgac gatggtccgt ccgaatggcc acagtgcata cgacgcgatg cgcagcgagg
    13021 ctattccgaa cgggatcgtg atgatcagga tgaagcacac cagtgccgcc agcagatagc
    13081 caagcgccag ccataggccg ccgaagacca accaaatgat gttcaggatc aggcgcattg
    13141 ctcctccagc tgtttctcca gcctaaccga gttaaacagg ctgctcggcc tagggcgagc
    13201 cgagtaggat cacgtcatcg cgtcctgcga ctggcggggc gcgttctttg cgagcgatag
    13261 acaagcaggt gaagccagtg ccgaccggca gggtgaagtg gtatgaccca gagaagggct
    13321 tcggcttcct gtcccaggag gacggcgagg acgtctacat ccggtcctct gctctgcccg
    13381 ccggcgtcga ggcgctcaag tccggccagc gggtggagtt cggcgtggcg tcgggccgtc
    13441 gcggtccgca ggcgctgagc ctcaagttga tcgacccgcc gccgtcgccg ctgcggacgg
    13501 gccgccgcga gaccgggccg gccgagcaca agcacacgcc cgacgagctg cacggaatgg
    13561 tcgaggacat gatcacgctg ctcgagggcg cggtgcagcc ggagttgcgc aaagggcgct
    13621 acccggatcg caagaccgcg cggcgggtgt cggaggtcgt caaggccgtc gcgcgcgaac
    13681 tcgacgcgta ggttctagcg ccgacttctg caccagggtc gtagattcag ccgatccaca
    13741 gccctggtgc agaactcgcg gaaacgggaa gactgaactc atggctgcct acgtcgccag
    13801 cggcggcgag ttcgaacggg acaccgacta catcaccacc cgcatcaccc cggacggacg
    13861 tgacggctat ccggtcgagc ccggccgcta tcggctgatc gtcgcgcggg cgtgtccgtg
    13921 ggccaaccgc gcgatcatcg tgcggcggct gctcggcctg gaaccagtgc tctccattgg
    13981 gttttgcggc ccgacgcacg acgagcgcag ctggaccttc gacctcgacc ccggcggcgt
    14041 cgacccggtg ctgaagattc cccgcctgca ggacgcctac ttcgccaggt tcccgaacta
    14101 cccgaaaggc atcacggtgc cggcgatcgt cgacgtgccc accggccagg tcgtcaccaa
    14161 cgacttcgcg cagatcaccc tcgacctgtc caccgaatgg cggcagtacc accgcgacgg
    14221 cgcccccgac ctctaccccg aaccgcttcg cgccgagatc gacgaagtca cgcagcgcat
    14281 ctacaccgag atcaacaatg gcgtctaccg atgcgggttc gccggatcgc agcgcgccta
    14341 cgaccgtgcg tacgaccggc tgttcaccgc gctcgactgg gtctccgaaa ggctaagcgg
    14401 ccaaaggttt ttggtgggcg acaccatcac cgaggccgac gtgcggctgt tcaccacgct
    14461 ggcacgcttc gacccggtct accacggcca cttcaagacc aaccggcaga agctcagcga
    14521 gatgccggtg ctgtgggcct acgcccggga cctgttccag acgcccgggt tcggcgacac
    14581 cgcggacttc gtccaaatca agcagcacta ctacatcgtt cacaaggaca tcaaccccac
    14641 ccggatcgtg ccgaagggtc cggacctgtc gaactggctg acgccgcatc atcgagaaga
    14701 actgggcggc aggtcctttg gcgacggaac accaccggga ccgcccatag agggggagcg
    14761 ggtggtgcca ggtcactcgc cgctggcctg aacccactcg gtgcgtaccg accactcggc
    14821 gtgcggcatc gggaacaagt cgcccttctc gtcctgcacg agtgtgagca attgcaccac
    14881 cacccggttg agcttgccgc cgttggaacc gacggtcgga atcgtgacgg ccaggcggct
    14941 gttcggccgg aatacatcca gcgtgctgtc tcgcgggtcg ttgtagaacg cccgcagcag
    15001 ccacggcgtg cggccgatcg aggtgggcac cgacagctgc accgggtcac gctcgtcgac
    15061 gccgagttcg ccctcggttt cgtagttctc gcagtcttcg agattcagca cattgcaata
    15121 gcggtacggg cccacgcgcg tcagttgccc tcgcgaatac gcgctgattt ccggacgttg
    15181 cggcggctga tcacgcacta gaagccaaat accaacggcc acgacgccac acaccaacac
    15241 gacgacagcc gccaacgcgg tgagcacacg tgtcactcgg gggtcaccat cgcgccgtcg
    15301 ggcgccctgc cctcctgctc cgccaccacc ggccggttac cgccgaagcc ggggatcaac
    15361 gagtcacctc ggtagctcac gatcgtctgc gccagaccca gaatcaggaa cgaggagatc
    15421 gcggtgaacc ccacccgcag atcggtgtac accagcacgc cggtggcacc gccggccacc
    15481 cacgccagct gcaacagtga ctccgaccgt ccgaacgccg acgcccgcga ctgctccggc
    15541 aggtcgtcct gcaggcaggc gtccagtgac gccttgccca ccgcgctgca gcccgacgtg
    15601 atcagcgcgg cgatcgccat gacgatcaga tcaccgaaga acgcagcggc aagggcggcc
    15661 gcggtgacca tggtcgcgca gcggaccacc agcagcgacg ggtgtcccag cttgagtcgc
    15721 gcggctagcc cgttgccggc gaagttgccg accgtggccg cggcgccgat catcccgagc
    15781 agcgcgacct ggtgccaccc gttctggccg gtctgcttgg cgacgaacgc cgggtacaag
    15841 aacaagaacc cgaccatgac cttgatggtg acgttgcccc acagcgacgt gatgatgttg
    15901 cggcccaaag gctgtcgcgg tcgtccggac ctgctgggcc gagatgctgt gttgccgtgg
    15961 ctgtctcggc gtagcggccc gctgcggccg tgatacgtca gcgtggtggg cacctcgccg
    16021 gtggtgacct ccacccagcg tggaatctgc atcgacaggt acgccccgac gacagccacg
    16081 acgacgatca cgaatagcgc gccgggcagg tggaacagct tggtaaacgt gtattcaaag
    16141 cctgcggcga tcccaccgcc gatgatcgtg ccgccgatca gcccgaagac ggtcagccgc
    16201 gaattgaccc gcaccagatc gatcgtcggc ggcatcaccc tcggggtgac cgcgctgcgc
    16261 agcacggaga acgacttgga caacaccatc atcaacagcg cgcacggata cagcacccac
    16321 ggcgggaagc tgccggtggc cgggtcgtag ttggcgatca tcacgatggc cagtgcggtg
    16381 cgcaacacga acgacatcgc caacgcgacg cggcgcccgt gctgcagccg gtcgagagcg
    16441 ggaccgatca gcggcgcaac aacggcgaac ggcgcgatcg tgatcaatag gtaaagcgcg
    16501 acgcggcttt tgctttcggc cgacgcggcc gcgaagaaca gcgtgttggc caacgcgacc
    16561 gccatcgcgg cgtccaccgc gaagttggcc atcaccggat acgtcaacgc ggtcagtccc
    16621 gacttgtcgg cgccgtcggc ggtggccgcc cgatggaaca ggccgtacat ccgcacgccc
    16681 atttcccggc tgcgcatcgc ggccgcgcgc gtcaccgtca ccttctgacc gcccggcgcg
    16741 ctggggccgg acctcggcgg cggggcgttg tggcgggccg gttcatccag cggcggcaga
    16801 tagcggttcg cgctcgccgt cggctcccgg cgcggtcggc ggtacggcgc atcgtcggtg
    16861 ggatagttcg ccatcccggg gtgttcgtcg gcgccgaacg gacgcggcgg atagtaccgg
    16921 cgatcgccct gatcaccgcc cagggcgtca cgccgcggtc cgctcacagg ttcgattgtc
    16981 cactatcggc gccgctcggt cgcgcagcta gtcggtgtgg ctacgggtcg gtgcgtcggg
    17041 cactataggg atgcgatgga cgacgtgaca gtggggctgg acggggcggt cgatcaggcg
    17101 cgagcggcga tcgccgagta cagcagcgag gacaccgttg gcgaatacct cggtgtgaac
    17161 tacgacgacg cccactctgc cacccatcgc ttccttgcca acttgcccgg ctactgcgga
    17221 tggcagtggg cggtcgtggc ggccgcccat cctggcgccg accacgcgac gatcagcgag
    17281 gtcgtgctgg taccgggtcc gaccgcgcta ctggccccgc gttgggtgcc gtgggaggcg
    17341 cgtgtccgac ccggcgatct ggggcccggt gaccttctcg ccccgcctcg cgacgacttg
    17401 cggctagttc ccggctacac ggccaccggc gacccggccg tcgacgacat cgcagtcgag
    17461 ttcgggctgg gccgacggca agtgctcagc ggatcgggcc gcgccggcgc cgcacagcgc
    17521 tggcgcgacg gcgagtacgg cccgcattcc gcgatggccc gatcggttcg ccgggtctgc
    17581 cgtgactgcg cgttctatct tccgctcgcc gggttgctcg gcaggatgtt cggcatatgc
    17641 gcgaacgaga tgtcggctga cgggcacgtc gtcgacgcgg aatacggctg cggcgcgcac
    17701 tctgacaccc cggcgccggc gggcaacggc tccccccgct acgacccgtt cgacgacggc
    17761 gtgctcgatc ttcttgaaaa ccaagagaat taacgccttt cggcgacggc tttgatccgc
    17821 gccagcgacg cgttcatgcc gtccaccagc tcttgttcga aattgggcac cccgccgagc
    17881 agcaggttga ccaagaagtt cgacgccggc ttgacaccgt tctcggcgtg ccgcgactcg
    17941 acgacccgcg tgccggtggc ggtcggttcg agctcgtagc tccagacggt gttgttcgtg
    18001 ttcacccgga acgcgaattt gcgttgcggc acaatctcgg tgatcgtgcc ggtggtcggc
    18061 cagaacagcc gattgcgccg gttcaggttg atcgtgcggg tgccctgacg cagcgagccg
    18121 agaggcctca tcagtcggca ctgcgggctc cattcgggca tccggcgcaa gtcggtgatc
    18181 aactgccaga ccttgtcgac cggcgcgtcg acgtcgatct gggcttgcaa aagcggtgcg
    18241 ggcattgacg actcctcggt ttcagcgcaa tccggtctga gcgcccctcg ctccccggcg
    18301 ggcggctcgg cgttgccaca agaacacgga cgtgccgaac cccgcaagac ccaggccggc
    18361 caccgcgaca ggtcgccagg tctcgagaga cggcaccgtg aaggccacga tgacggcgac
    18421 gatccacgcg agcgtgcaca ccgcgatcac cggccgggga tcaagcagag catcaggcag
    18481 cgacggtggt tgcacctcgc tctggtcggc aggctcgtct gtcatcgtga ccaacggtac
    18541 cgttcggccg tggcccacct cgtcgacgtc gccaatccgg ctgacgcacg gctcgacgac
    18601 ttccgcaacc tcaacagcat cgaccgtcgc ccggacctgc ccagcggcaa ggggctggtg
    18661 atcggtgaag gcgtgcttgt ggtccagcgc atgctggcgt cgcggttctc accgttcgcg
    18721 gtgatgggca acgaccgccg actcggggat ctgcgtgaag accttgcggc cgtggatatt
    18781 ccgtactacc gggtggacgc cgacgtgatg gcacaggtgg tgggttttca tctgaatcgc
    18841 ggcgtgctgg cggccgcgcg tcgcccgcca atgctcacgg tgcgggacgt gatcaaggac
    18901 gcccgcactc tggccgtgtt ggagggcgtc aacgatcacg agaacctcgg gtcgatcttc
    18961 cgcaatgcgg cgggcctcgg tgtggacgcg gtggtgttcg gcccgggctg cgccgacccg
    19021 ctgtatcgcc gagcggttcg ggtctcgatg ggacatgcct tgctggtgcc ctgcgccagc
    19081 gtcgagacct ggcctgctga tctaaacctc ctgtgggaga acgggtttca actccttgcg
    19141 atgacgcccg atcccgacgc caccacgctg gccgacgcga tggcgcacgt cggtgacggc
    19201 aaggtggcga ttctcgtcgg cgccgagggc ccgggtttga cgcataaggc gatgcgggcc
    19261 agcgacatgc gggtgcggat tccgatgtcg cgcggcaccg actctctcaa cgtcgcgacg
    19321 gctgcggcgc tggccttcta tgagcgggtt aggctcgcgc ggtgaccgac gatgagccgc
    19381 ttgcgcgaag agcgataacc gacgatgagc cgcttgcgcg aagagcgata accgactaca
    19441 gcacgccgtg ggggacgggg ctgacggtcg cggccttcgt cgccgccgtc accgccgcgg
    19501 cgatcgtggt gctgagcttg ggggtgtccc gggttcatcc gctgttggcc gtcgcgctca
    19561 atctcatcgc ggtgggcggc ctcgcgccga cgctatgggg atggcgcgcg gtcccggtgc
    19621 tgcgctggtt cgtgctcggt ggcggcgtcg ggattgtcgg cgcgtggctg gcgctgctgg
    19681 cgctcgccgt gtgaccgccg cgccgaaacg gtattccacg tggtcgctac tcgaagaaat
    19741 ccgcggtagt taccgtttcg gcgctcgccg cgaatcagtg gtgtccgccc gagcggacgt
    19801 cgagcagcac gtcctcccac gtcggcaccg ccggcttgct tcggcgcgcc cgaggcggcg
    19861 tcaccggctc ttcaactgcc agctcttgtg gcgcggcgtc gtcgaagtcg agttgagcga
    19921 ccggcgctac cggccgcagc gggcgatcga agtccgggtc gatcagttcc tttgccgggt
    19981 cgtcgagcgc gaccgcggtg ccgccgtggg cgccgggcgt gaaccggaag tgcgcgacgt
    20041 tttgcgagcg gccggccagc caggtcagct gcacggtcca gcggccgtcg gcattcttcc
    20101 acgcgtccca cgacgtcgcg tcatggtcga ggccgcgccc gaccagagcg gtggccaccg
    20161 tttcgagcag cgtctgaacc gaggggccgt cggccaacat cgggtgcgac gcggttgcca
    20221 gctccgcggc acgcgatcgc tccaacagca ccgggtgggc gaaccgctcc actcgtgaca
    20281 cgtccacccc ggccgacgtg gcgacgtgtt cgaccgaggc accggctcgg atgcgggatt
    20341 gaatctcctt gggactgagc acgtttgtga cctcgatgtc gatttcggtt tggctgctgc
    20401 gggtgccttc cccgcggaca gccgcacgca gccggtcatc ggcccgcaac aggaacttct
    20461 cgccaggtcg cgtgctctcg cagatgatgt gtttgccgtc gacatcgagg ctgacgaccg
    20521 tgagttcctg catgccgacc tcctccgggc cgtcacgctg aggtcttctt gctcctcgcg
    20581 tgcgcacccc gtcagctgcg cactttagac gctcaatagg ccaatccggc gctgacacgc
    20641 tggctagagg tagagacgtt cgaccaccca gtcgacgcac tcggtcagcg cgctaacgtc
    20701 gtcgggctcg acggccggga acatcgccac ccgcagctgg ttgcggccga gtttgcggta
    20761 cggctcggtg tcgacgatgc cattggcccg caggatcttc gcgacggcgc tcgcgtccac
    20821 gtcgtcggag aacccttggt ccctctaggt ggctggtgtt ttgcatggca aacaatcttc
    20881 cacca
//
LOCUS       DASWBF010000451         3153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_373096,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000451 DASWBF010000000
VERSION     DASWBF010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373096"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..672)
                     /locus_tag="VF874_16600"
     CDS             complement(229..672)
                     /locus_tag="VF874_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177621.1"
                     /translation="MTDREPSQLVSEAADNLRIFNHQTRTEQLTDRCIIAPDAYTILG
                     SATELAYSLPQALSQLSDALRRSLAVFDIYDQDRDPADSVTIATHAISAAADAFTQAA
                     QHLSDAQAAIAHQGVNEDSAGNLRRRPTLRLAPSGPDSDSPPRCC"
     gene            complement(704..1009)
                     /locus_tag="VF874_16605"
     CDS             complement(704..1009)
                     /locus_tag="VF874_16605"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX9177622.1"
                     /translation="MSTELNDLLNEARELENADSSDYTAATAAHPNRDKAKTLTVRLT
                     DDEMVRLRATAAERGLPVSAVVRAAIAHELQDDDGDSTRALMTALRDHNLAIVRMAK"
     gene            complement(1006..1293)
                     /locus_tag="VF874_16610"
     CDS             complement(1006..1293)
                     /locus_tag="VF874_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018118188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9177623.1"
                     /translation="MAELIWPADAVTHMWDQHQTTEAMAVEALTDPDVVAFVPDPKSR
                     TGKSSRYIGFSATADRVLVVIVVEHQGQHYGANCWPANLTDQRIYREENTP"
     gene            1596..2201
                     /locus_tag="VF874_16615"
     CDS             1596..2201
                     /locus_tag="VF874_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9177624.1"
                     /translation="MIWSLVHTKGGVAKTTTAMFLAAAAVRRGVPARVVDADPQGSAS
                     SWADRAVHLGTPLPFDVSPATAASLRELSSEPGKLVLVDTPPGTAAAIDAAIDVADLV
                     IIPTGPRAADIDRVWPTLDITQHRPTTILLTLVDMRKVEAVEIPRGLAEADAPVLRSI
                     VRNLTAIERAFGRGIPRYLGDYGDVYDELMSAAALARAVTV"
     gene            2198..2644
                     /locus_tag="VF874_16620"
     CDS             2198..2644
                     /locus_tag="VF874_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177625.1"
                     /translation="MTAKSSTADVVRASVIRRPTGAAAAARAASTFQGGDAQRTDAAA
                     VVHEAMRTTMHLPPAMRTALKRRALDRDTTMSGLIRAAISSGLQDPAALAGASMEHRR
                     TSSGARTTLDLPRDVHRTLKRLAADEDTSVQALVLAAIVRAYPDLT"
BASE COUNT          453 a          907 c         1167 g          626 t
ORIGIN      
        1 ttccgctcgg ccgccatctc gcgcagccgg gcgtacacga ccgcggcggc acgtactttg
       61 atctgttcgc gatcaccttt gtgcggttgg gccatgggat tgatcatgtc gcgaccctgg
      121 gcttgtacct ggtaccgaca cgcggtcaat gcgtgatgtt ttcgtgatcg actgtgcaga
      181 ccgtcgacct ttacccggct ggacgaggat ttctttgacg gactgtgatc agcagcacct
      241 tggcggtgag tcggagtctg gtccactcgg ggctagccgg agtgtgggtc gccggcgtag
      301 gttgccggcg ctgtcttcgt tgactccttg gtgtgcgatg gcggcttggg cgtctgaaag
      361 gtgttgggct gcttgggtga aggcgtcggc ggccgcggat atcgcatggg tggcgattgt
      421 cacgctgtcg gctgggtcgc ggtcctggtc gtagatgtcg aagacggcaa gcgagcggcg
      481 cagcgcgtca ctgagttggc tgagggcctg cggcagcgag taggcgagct cagtggccga
      541 cccgaggatt gtgtaggcgt ccggggcgat tatgcatctg tcggtgagtt gttcggttcg
      601 ggtttggtgg ttgaagatcc ggaggttgtc ggcggcctcg gacaccagtt ggctgggctc
      661 tcggtcagtc atggtcggtc ggtctcgttt ccgcgaattc ggttcacttc gccattcgga
      721 cgatggcgag gttgtggtcc cgtagcgcgg tcatcagcgc acgggtgctg tcgccgtcgt
      781 cgtcttgcag ttcgtgggcg atggctgccc ggacgactgc agatacgggt aggccgcgtt
      841 cggcggcggt ggcgcggagc cggaccattt cgtcgtcagt gagccgcacg gtaagggttt
      901 tggctttgtc gcggttgggg tgggcagccg tggcggcggt gtagtcggag ctgtcggcgt
      961 tctccagctc gcgtgcctcg ttgagtaggt cgttgagttc ggtgctcatg gggtgttctc
     1021 ttctcggtat atgcgctggt cggtgaggtt ggcgggccag cagttcgcgc cgtagtgctg
     1081 gccctggtgc tcgacgacga tgacgacgag cacacggtcg gcggtggctg agaacccgat
     1141 gtaccggctg ctcttgcccg tgcggctctt ggggtcggga acaaacgcca ccacgtcggg
     1201 gtcggtgagg gcctcgacgg ccatggcctc ggtcgtctgg tgttggtccc acatgtgggt
     1261 caccgcgtcg gcgggccaga tcagctcagc cacgagccaa tcgtattaca tatgtatgac
     1321 atgtcgaccc tccttgtcga ctgccccata aagagccacc aattgtgctc tccgaccccc
     1381 acaccgctcc ccctcccgga taagggcggt gtccgtgtgc ggtgcggggc ttgtccaggc
     1441 tcggctttcg accgcgtagc ggcgtaggcc ttgacagcgc agcggcgcac gttgaggaca
     1501 attcccggct tccgggaggg gaagcgagca gagagctgcc atatgcgggc gtgtacacgc
     1561 atatggatgc gcgtatggat gtatgttgtt gcatcgtgat ttggtcactt gtgcatacga
     1621 agggtggcgt cgcgaagacg acgacggcga tgtttttggc ggcggccgcg gtgcggcgcg
     1681 gtgttccggc tcgtgtcgtg gatgccgatc cgcaggggtc ggcgtcgtct tgggctgatc
     1741 gagcggtgca ccttggcacc ccgctgccgt ttgatgtcag cccggcgacc gcggccagtc
     1801 tgcgggagtt gtcgagcgag cccggcaagt tggtcctggt ggataccccg ccggggacgg
     1861 cggcggcgat cgatgcggct atcgatgtgg cggacctggt gatcattccg accggtccgc
     1921 gtgcggcgga tatcgaccgg gtctggccga cgctggatat cacgcagcac cggccgacga
     1981 cgatcctgct cacgctggtg gacatgcgga aggtggaggc ggtggagatt ccgcgcggtc
     2041 tggctgaggc cgatgcgccg gtgctgcgga gcatcgtgcg gaacctcacc gcgatcgagc
     2101 gggcgttcgg ccggggcatc ccgcggtact tgggtgacta cggggacgtg tacgacgagc
     2161 tgatgtcggc ggcggcgctt gcgagggcgg taacggtatg acggcgaagt cctcgacggc
     2221 tgatgtggtg cgggcctcgg tgatccgccg gccaaccggg gccgcggcgg cggctcgggc
     2281 ggcaagcacg tttcagggcg gcgatgctca gcgcaccgac gcggcggcgg tcgtgcacga
     2341 ggcgatgcgc acgacgatgc acctgccccc ggcgatgcgg acggccctca agcggcgcgc
     2401 gctggaccgg gacaccacta tgagcgggct gattcgcgcg gcgatcagct cggggctgca
     2461 ggaccccgcc gcgctggccg gtgcgtcgat ggagcaccgc cgcaccagta gcggtgctcg
     2521 gacgacactc gatttgccgc gcgatgtgca tcggacgctc aaacgtttgg cggctgacga
     2581 ggacacaagc gtgcaagctc tggtcctagc ggcgatcgtg cgggcctacc ctgacctcac
     2641 gtgatgcggg tatggaagta catatgcgcg tcaatactga cgcgcatatg tacttcagct
     2701 cctcccttcg gagacggccg ccgtggggga ttggaccgga agcggacgcg cgaacgcgat
     2761 cccgcacccc gccgagtgtg gtcggcgagg tgcatgttcg gtgcatgttc ccgtcgcgga
     2821 catccgaggg cggcagcgcc gactagctcg cggcgtggcc agagatccgc cagtttcggg
     2881 gtatttggga catgctcgac tggcgctgga aggccggtcc atgcaagacc tgctgaccga
     2941 gtacctgcgt gactggctcg gcgcagactg aaaagttggc tgtacggcgg cggcgaaggc
     3001 ctgccagtag tcctggccct gacctggcat ccggccgctg ccctccccgt tagaggcctg
     3061 attcaaggga ccgcccgaag gagtcaaggg tcgtgatcgc cgtaggcgac ggccgcaggc
     3121 cgcccttcac gccgtaggac ggtcccgcac aat
//
LOCUS       DASWBF010000452         6332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1092671,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000452 DASWBF010000000
VERSION     DASWBF010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092671"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..311)
                     /locus_tag="VF874_16625"
     CDS             complement(21..311)
                     /locus_tag="VF874_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177626.1"
                     /translation="MKRIPLASAVAVGSIAAALAFGTNAFDPVIAGAQPNNGSDPAQE
                     WDIELYDSCIKMYDPGTAPVTLSEWDRYCCDHTGGVWNGSKCEAPAGEPAQS"
     gene            452..1792
                     /gene="glnA"
                     /locus_tag="VF874_16630"
     CDS             452..1792
                     /gene="glnA"
                     /locus_tag="VF874_16630"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9177627.1"
                     /translation="MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGA
                     FEEGIGFDGSAIEGFARVSESDCVARPDPSTFQILPWTTSGGEQHSARMFCDITMPDG
                     SPSWADSRHVLRRQLAKASDLGFSCYVHPEIEFFLLKSSSEDGTPPVPTDSDGYFDQA
                     VHEAAPNFRRHVIDALESMGISVEFSHHEGAPGQQEIDLRYADALSMADNVMTFRYLV
                     KEVAIGEGVRATFMPKPFGEYPGSAMHTHMSLFEGEVNAFHSPDDALQLSDVAKSFIA
                     GILEHATEISAVTNQWVNSYKRLVVGGEAPTSACWGAANRSALVRVPMYTPRKTSSRR
                     IEVRSPDSACNPYLTFAVLLAAGLRGVEKGYALGPQAEDNVWNLTPEERSAMGYKELP
                     GSLGVALEQMENSELVAEALGEHVFDFFLRNKRAEWENYRRQVTPYELKTYLSL"
     gene            1802..4762
                     /locus_tag="VF874_16635"
     CDS             1802..4762
                     /locus_tag="VF874_16635"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX9177628.1"
                     /translation="MAKPATQRPRLPSPGRLGLVEAPARTDLSRLGWTSDDYVELLWS
                     LSRAPDADAALRAMVRLADALGDGYQELNAALVKDRSLRGRLFALLGSSLALGDHLVA
                     SPQTWRLLQGNVSLPSADELRRQFVELVTEKPRDATLPALRTLYRNRLLVLAALDVAS
                     TVENEPVLPFATIGAHLSELADAALAGALEVAVLRVCQDGTRPRLAVIAMGKCGAREL
                     NYVSDVDVIFVGEKADALETRVAGEMMRLASETFFEVDAALRPEGKAGALVRTLESHV
                     TYYKRWAKTWEFQALLKARPAVGDAELGEQYMAALMPMVWTASEREDFVPEVQAMRRR
                     VEELVPADVRDRELKLGAGGLRDVEFAVQLLQLVHGRTDEALHVSSTVDALAALGAGG
                     YVGRDDAANLTASYEFLRLLEHRLQLQRLKRTHMLPKPDDDEAMRWLARAAHVRPDGR
                     HDALGVLREELKRQNMRVSRLHAKLFYQPLLEFVGPGLELAGGMSTEAAERQLAALGY
                     QAPQNALTHLAALTSAGGRRGRVQSVLLPTLLDWLSNTPDPDAGLLAYRKLSEALSDQ
                     RWFLATLRDEGAVAKRLMHVLGTSAYVPELLMRAPETIQSYADGPTGPKLLDVDTEHV
                     ARSLLAATSRHADPVKAIAAARSLRRRELARIASADLLGLLEVRQVCDALTSVWVAVL
                     QSALDTVIRFNTPDTGEAPARISVIGMGRLGGHELGYGSDADVMFVCEPVSGADESAA
                     VRWSTTIAEQVRALLGTPSVDPPLEVDANLRPEGRNGPLVRTLNSYAAYYAQWAQPWE
                     VQALLRAHRVGGDEDLGQRFLLMADKTRYPQGGVSAESMREIRRIKARVDAERLPRGA
                     DPNTHTKLGRGGLADVEWTVQLLQLRHAHKVPGLHNTSTLETLDAIGAAELIVESDVE
                     LLRQAWLTATRARNALVLVRGKASDQLPGPGRLLNAVAVAAGWPTPDGGEFLDYYLRA
                     TRRAKVVVRKVFGS"
     gene            complement(4759..5262)
                     /locus_tag="VF874_16640"
     CDS             complement(4759..5262)
                     /locus_tag="VF874_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9177629.1"
                     /translation="MTSPNLDARLNVHSPKIISVFRIIVGLLFALHGSMILFGWPLPM
                     PMQTHVGDWPGWWAGLIEFVTGLLVMVGLFTRPAAFVASGQMAVAYFWQHWPFNEGEK
                     VFWPFDKQMGGNSGELAILFCFSFLLILFIGPGRYAVDTMRRRRPIATTRVPAARRPG
                     LLSRFRR"
     gene            complement(5272..5706)
                     /gene="dtd"
                     /locus_tag="VF874_16645"
     CDS             complement(5272..5706)
                     /gene="dtd"
                     /locus_tag="VF874_16645"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017206132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX9177630.1"
                     /translation="MRVLVQRVTSASVTVDGEVVGVIRPDGQGLLAFVGVTHNDTDEI
                     AQRLAEKLWQLRILDDERAAADVNAPILVVSQFTLHANTEKGRRPSWNAAAPRPVAEP
                     LVTAFADALHRLGATVATGMFGAHMQVELVNDGPVTVMLELE"
     gene            complement(5716..6168)
                     /locus_tag="VF874_16650"
     CDS             complement(5716..6168)
                     /locus_tag="VF874_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9177631.1"
                     /translation="MTVYQAVVVGTDGSESSPRAVDRAAEIAAESNAKLFIATGFLPQ
                     REDLRAADILKDEGWRVHGNAPIYAILREAHARAHAAGAKNIEERPIENAPVHALVDL
                     AEDVGADLLVVGNVGLDARSAILGRMFSIPGSVATRAKIDVLIVHTTD"
     gene            complement(6212..>6332)
                     /locus_tag="VF874_16655"
     CDS             complement(6212..>6332)
                     /locus_tag="VF874_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177632.1"
                     /translation="GGVFTEDLIETWIAYKREQEILPVQVRPHPYEFALYFDV"
BASE COUNT         1038 a         2035 c         2198 g         1061 t
ORIGIN      
        1 ggggggatct gtcgggtcgg ttacgactgc gccggttcgc cggctggcgc ctcacacttg
       61 gagccgttcc acacgccgcc cgtgtgatcg cagcaatacc tatcccattc gctgagggta
      121 accggcgccg ttccggggtc gtacatcttt atgcacgagt cgtaaagctc gatgtcccat
      181 tcttgggcgg gatcactgcc attgttgggc tgggcgccgg cgatcacggg gtcgaatgcg
      241 ttggttccga aagcgagcgc ggcggcgatg gagccgacgg cgactgccga ggcgagtggg
      301 atgcgtttca ttgttgtgtc tccgtacagt tagcggcggc ccggggttgc gccgccgtca
      361 taggtaggag ccctcggtcc tcacagtggt gagatttttt tcgcaccaag cgcgtaacac
      421 agaattaaca gccgctgaat acgctgccgt catggatcgt cagaaggaat tcgtgctgcg
      481 cacgctcgag gaacgggaca tccggttcgt gcggttgtgg ttcaccgacg tgctgggatt
      541 cctcaaatcg gtggcgatcg cgcccgccga attggagggg gcgttcgaag aaggcatcgg
      601 cttcgacggc agcgcgatcg aagggttcgc ccgggtgtcg gagtccgact gcgtggcccg
      661 gccggacccg tcgacgtttc agatcctgcc ctggaccacc agcggcggtg agcaacactc
      721 ggcgcggatg ttctgcgaca tcacgatgcc cgacggctcg ccgtcgtggg ccgattcacg
      781 tcacgtgctg cgccggcaac tggccaaggc cagcgacctc gggttctcct gctacgtgca
      841 ccccgagatc gagttcttcc tgctcaagtc ctcatcggaa gacggcacgc cgccggtccc
      901 caccgacagc gacggctatt tcgaccaggc cgtgcacgag gcggcgccca acttccggcg
      961 gcacgtgatc gacgctctcg agtcgatggg catctccgtg gagttcagcc accatgaagg
     1021 cgcacccggt cagcaggaga tcgacctgcg ctacgccgac gcgctgtcga tggccgacaa
     1081 cgtgatgaca ttccgctatc tggtcaagga agtcgcgatc ggcgaaggtg tgcgggcgac
     1141 gttcatgccc aagccgttcg gcgaataccc cggctcggcg atgcacaccc acatgagcct
     1201 gttcgagggc gaggtcaacg cgttccacag ccccgacgac gcgctgcaac tctcggacgt
     1261 cgccaagtcg ttcatcgccg gcatcctcga gcacgccacc gagatcagcg ccgtgacgaa
     1321 ccagtgggtg aactcataca agcggctggt ggtcggcggc gaggcgccga cctcggcatg
     1381 ctggggtgcg gccaaccggt cggcgctggt gcgggtgccg atgtacacgc cccgcaagac
     1441 gtcgtcgcgg cgcatcgagg tgcgcagccc tgactcggcg tgcaacccgt acctgacgtt
     1501 cgcggtgttg ctcgccgccg ggctgcgggg cgtggagaag ggctacgcgc tcggaccgca
     1561 ggccgaggac aacgtgtgga acctgacacc cgaagaacgc agcgcgatgg gctacaagga
     1621 actgcccggc agtctgggcg tcgcgctgga gcagatggag aactcggagc tcgtggcgga
     1681 agcgttgggc gagcacgtct ttgacttctt cctgcgcaac aagcgcgctg aatgggagaa
     1741 ctaccgtcgc caagtgacgc cgtacgagct gaagacgtac ttgtcgctgt aacgtcaggg
     1801 cgtggccaaa cctgcgaccc agcgaccgcg gctgccgagt ccgggccggc tgggactggt
     1861 cgaggcgccc gcgcgcaccg atctgtcccg gttgggctgg acatccgacg actacgtgga
     1921 actgctctgg tcgctgtcgc gagctcccga cgcggacgcc gcgctgcgcg cgatggtgcg
     1981 gctggccgac gcgctgggcg acggctatca ggagctgaac gcggcgctgg tgaaagaccg
     2041 ctcgctgcgg ggccggctgt tcgcgttgct cgggtcctcg ctggcactcg gcgatcatct
     2101 ggtggccagc ccgcagacct ggcgcctgct gcaggggaat gtgtcgctac cgtcagccga
     2161 cgaactgcgc cgccagttcg tcgaacttgt cactgaaaaa ccaagggacg caacgcttcc
     2221 agcgctgcgc acgctctatc gcaaccggct gctggtgctc gccgcactgg acgtggcgtc
     2281 gacggtggaa aacgaaccgg tgctgccgtt cgcgacgatc ggcgcccacc tgtccgagct
     2341 ggccgacgcc gcgctcgccg gcgcgctcga ggtggcggtg ctgcgagtgt gccaggacgg
     2401 gacgcggccc cggctggccg tgatcgcgat gggcaaatgc ggtgcgcgcg aactgaacta
     2461 tgtcagcgac gtcgacgtga tcttcgtcgg cgagaaggct gacgcgctgg agacccgcgt
     2521 ggcgggggag atgatgcggc tggcgtcgga gacgttcttc gaagtcgacg ccgcgctgcg
     2581 gcccgaaggc aaggcggggg cattggtccg cacgctggag tcgcacgtca cctactacaa
     2641 gcggtgggcc aagacgtggg agttccaggc gcttttgaag gcacggccgg ctgtcggcga
     2701 cgccgaactc ggcgagcagt acatggccgc gctgatgccg atggtgtgga ccgcctccga
     2761 gcgggaggac ttcgtgcccg aggtgcaggc catgcggcgg cgcgtcgagg agctggtgcc
     2821 cgccgacgtc cgggaccggg agctgaagct cggcgcgggc ggcttgcgcg acgtcgagtt
     2881 cgcggtgcag ctgctgcaac tcgtgcacgg ccgcaccgac gaggcgctgc acgtgtcgtc
     2941 gacggtcgac gcgctggcgg cgttgggcgc gggcggctat gtgggccgtg acgacgccgc
     3001 gaacctgacc gcgtcgtacg agttcttgcg gctgctcgag caccgcctgc agctgcagcg
     3061 gctcaagcgc acgcacatgc tgcccaagcc cgacgacgac gaggcgatgc gctggctggc
     3121 ccgcgccgcg cacgtgcggc ccgacggtcg ccacgacgcg ctcggggtgc tgcgcgagga
     3181 gctcaagcgg cagaacatgc gagtgtcgcg gctgcacgcg aagctgttct atcagccgct
     3241 gctcgaattc gtcgggcccg gactggaatt ggccggcggc atgagcaccg aagccgccga
     3301 gcggcaactc gccgcgctcg ggtatcaggc gccacagaac gcgctcaccc atctggccgc
     3361 gttgaccagt gccggcgggc gccgcgggcg cgtccaatcg gtgctgctcc cgacgctgct
     3421 cgactggctg tccaacacgc cggatcctga cgccgggctg ctggcgtatc gcaagctgag
     3481 cgaggcgctt tcggatcagc ggtggttttt ggccaccctg cgcgacgagg gcgcggtggc
     3541 gaaacggctg atgcacgtgc tgggcacgtc ggcctacgtg ccggaactgc tgatgcgtgc
     3601 gccggagacc atccagtcct acgccgacgg cccgaccgga ccgaagctgc tcgacgttga
     3661 caccgaacac gtggcgcgtt cgctgttggc cgccacgtcg cggcacgccg atccggtgaa
     3721 ggcgatcgcg gcggctcgga gcctgcgccg acgggaattg gcacgcatcg cgtcggcgga
     3781 cctgctcgga cttctcgagg tacgacaggt gtgcgacgcg ctgacgtcgg tgtgggtggc
     3841 ggtgctgcag tcggcgctcg acacggtgat ccggttcaac accccggaca ccggcgaggc
     3901 gccagcgcgg atttcggtga tcgggatggg ccgcctgggt ggtcatgagc tcggttacgg
     3961 gtctgacgcc gacgtgatgt tcgtctgcga accggtctca ggcgctgacg agtcagcggc
     4021 ggtgcgatgg tcgaccacga tcgccgagca ggtgcgggca ctgttgggca caccgagcgt
     4081 cgacccacct ctggaggtcg atgcgaacct gcggcctgag ggccgcaacg gtccgttggt
     4141 gcgcacattg aactcttacg cggcgtatta cgcacagtgg gcgcagccgt gggaggttca
     4201 ggcgctgctg cgtgcgcatc gcgttggggg cgacgaagat ttgggtcagc ggttcctgct
     4261 gatggccgac aagacgcgct acccgcaggg cggtgtttct gcggagtcga tgcgggagat
     4321 tcggcgcatc aaggcgcggg tggacgctga gcggctgcct cgcggtgccg acccgaacac
     4381 gcacaccaaa ctcggccgcg gtgggttggc cgacgtggag tggaccgtgc agttgcttca
     4441 gcttcgccat gcgcacaagg tgcctgggct gcacaacacc tcaactctgg aaacgttgga
     4501 cgcgatcggc gccgcagagt tgatcgtcga gagcgatgtc gagttgctgc ggcaggcgtg
     4561 gctgacggcg acccgggcgc gcaacgcgct cgtgttggtg cggggcaaag ccagcgacca
     4621 gctgccagga ccgggccggc tactcaacgc ggtagccgtc gccgcgggct ggcccacccc
     4681 ggacggcggc gaattcctgg actactacct gcgcgccacc cgccgcgcaa aggtcgtggt
     4741 gcgcaaggtg tttggcagtt agcgccggaa acggctgagc agacctgggc gccgggcggc
     4801 cgggacccgt gtggtggcaa tgggccgccg ccggcgcatc gtgtcgacgg cgtaccggcc
     4861 gggaccgatg aacaggatca acaagaacga gaaacagaac aggatcgcca gctcgccgct
     4921 gttaccgccc atttgcttgt cgaacggcca gaaaaccttc tctccttcgt tgaacggcca
     4981 gtgctgccag aagtaggcca ccgccatctg gccggatgcg acgaaagctg ctgggcgggt
     5041 gaacaatccg accatcacca gtagtcccgt cacgaattcg atgagtccgg cccaccatcc
     5101 tggccagtca ccgacatgtg tctgcatcgg catcggcagc ggccagccga acaagatcat
     5161 cgatccgtgc agcgcgaaca ggaggccgac gataatccgg aagacgctga tgatcttggg
     5221 ggagtggacg ttgagcctgg catcgaggtt cggtgacgtc atggcccgac cttactcaag
     5281 ttcgagcatc acggtcaccg gtccgtcgtt gacgagttcg acctgcatgt gcgcgccgaa
     5341 catgccggtc gcgaccgtgg cgcccagcct gtgcagcgcg tcggcgaacg ccgtcaccaa
     5401 gggttctgca acgggtcgcg gtgcggcggc gttccacgac ggtcggcgcc ccttttcggt
     5461 gttcgcatgc agcgtgaact ggctgaccac cagaatcggt gcgttcacgt cggctgccgc
     5521 gcgttcgtcg tcgagtatcc gcagctgcca aagcttttcg gccagccgct gcgcgatctc
     5581 atcggtgtcg ttgtgcgtga ccccgacgaa cgcgagtagc ccctggccgt cgggtcgtat
     5641 tacgccgacc acctcgccgt cgaccgtcac gctcgctgac gtcacccgct gcacgagaac
     5701 ccgcatgggg ctccgtcaat cggtggtgtg gacgatcagc acgtcgatct tggccctggt
     5761 ggcgacactt ccggggatcg agaacatccg cccgaggatg gcggatcgcg cgtcgagtcc
     5821 cacattgccg accaccagca gatcggcgcc aacgtcctcg gcgaggtcta ccagcgcatg
     5881 taccggagca ttctcaatcg gccgctcctc gatgttcttc gctccggccg cgtgggcccg
     5941 tgcgtgtgcc tcgcgcaaga tcgcgtagat cggcgcgttc ccgtgcaccc gccagccctc
     6001 gtccttgagg atgtcagccg cgcgcagatc ttctctttgc ggcaggaacc ccgtcgcaat
     6061 gaacagcttc gcgttcgact cggcggcaat ctcggctgcg cgatcgaccg cgcgcggcga
     6121 cgactccgag ccatcggtgc cgaccaccac cgcctgatag accgtcattt gcgcctcccc
     6181 agcatcgtcg caatgccgag acagtaacgc gtcagacgtc gaagtacagc gcgaactcgt
     6241 acgggtgcgg ccgcacctgg accggcagga tctcttgctc ccgcttgtag gcgatccacg
     6301 tctcgatcag gtcctcggtg aacacgccgc cc
//
LOCUS       DASWBF010000453         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_965503,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000453 DASWBF010000000
VERSION     DASWBF010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965503"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VF874_16660"
     CDS             complement(<1..706)
                     /locus_tag="VF874_16660"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX9177633.1"
                     /translation="MTIDKMLSGNSIRLQACPIADGGTPVEVWTGWDHGFTSLDVADC
                     PGLVIVSPHPDDETLGFGATTAMLSEAGVDVHLVSVSDGGAAYPGLSAERRAQLETVR
                     RAEVRRAAKLLGAGEPIRLEMPDGELHRCERQISDAIAAILARFPPGTWCAATWRGDG
                     HPDHEATGRAAATAAAASGAVFVEYPVWMWHWARPGDPAVPWHRTRRVALTDRALRLK
                     RRATQCFASQVEPTQDG"
     gene            complement(693..1643)
                     /locus_tag="VF874_16665"
     CDS             complement(693..1643)
                     /locus_tag="VF874_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9177634.1"
                     /translation="MTTSLVRQWLDAGELNLPLPASGDTVGRWLKLAEFTEVDVVAGR
                     LAEAHTDAVAILAELGGPSAQPGQLWGVWAAEAPGQRLTAGHDGDAVALGGVKPWCSG
                     AGLCSHALVTAHGVDGNRELFAVDLAQPGVTPLASSWRNAGMATSDTRSVSFDDAPAM
                     PIGAPGDYLARPGFWHGAIGVAACWLGGARAVAAPLYERAASGCAGDHTLAHLGAVDA
                     AFAAAEATLAAAADHIDADPGNRSGLAELIARRVRAVVESAVEEALARTDRALGPGPL
                     CLDERHARAVADLTVYVRQSHAERDLAALGLLTATSRDHR"
     gene            complement(1640..2365)
                     /locus_tag="VF874_16670"
     CDS             complement(1640..2365)
                     /locus_tag="VF874_16670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9177635.1"
                     /translation="MTRAGFDRAVVVIPAHNEATRLRRCLTAVLGAATAMPGRVLTVV
                     VLDACTDRSADVAEGFGPGVQVLSIDAHNVGAARAAGFNRARTVWPDANVARTWYATT
                     DADSRVDPDWLVRQMVADADMVLGVVRVANWRHVPTSVAGRYLHRYRSKSGSDGAGHN
                     HIHGANMGFAAEKYWEVGGFAALPSGEDVDLVRRFELSGFHIRCDASLSVVTSARHEG
                     RAPNGFAAHLRSMLHRRGAEDLA"
     gene            2474..>2599
                     /locus_tag="VF874_16675"
     CDS             2474..>2599
                     /locus_tag="VF874_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX9177636.1"
                     /translation="MSAEDKLSNKIDDMGGKAKEAAGKLTGDDSTKNEGKLDQAKA"
BASE COUNT          467 a          919 c          834 g          379 t
ORIGIN      
        1 agccgtcctg cgtgggctca acctggctcg cgaagcactg tgtggcacgc cgtttcaggc
       61 gaagcgccct gtcggtcaag gccactcggc gggtgcggtg ccacggcacc gcaggatcac
      121 cgggtcgggc ccagtgccac atccacaccg ggtattcaac aaacaccgca ccgctggccg
      181 ctgcggcggt cgcggctgcc cgaccggtcg cctcatggtc ggggtggccg tcaccgcgcc
      241 aggtggctgc gcaccacgtt ccgggcggga agcgggcaag gatcgcggcg atcgcatcgc
      301 tgatctgccg ttcgcagcgg tgcagctcac cgtctggcat ctccagccgg atcggctcgc
      361 cggcacccaa cagctttgca gcgcggcgaa cctcggccct gcgcaccgtt tccagctgtg
      421 cgcgacgctc ggccgacagc cccggataag cggcgccccc gtcggaaacc gaaaccaggt
      481 gcacatcgac gcccgcctcg ctgagcatcg cggtggtcgc cccaaagccc aacgtttcgt
      541 cgtcgggatg cggggagacg atcaccaggc ccggacagtc ggcgacgtcc agggatgtaa
      601 atccgtggtc ccacccggtc cacacctcga cgggagtccc gccgtccgcg atcgggcagg
      661 cctgcaaccg aatgctgttg ccggacaaca tcttatcgat ggtcacggct ggtggctgtc
      721 agcaggccga gggcggcgag atcgcgctcg gcgtggcttt gccggacata caccgtcaga
      781 tcggccaccg cacgggcgtg ccgttcgtcg aggcagagcg ggcctggccc gagcgcccga
      841 tccgttctcg cgagcgcctc ctcgactgcc gattcgacga ccgcgcggac gcgccgggcg
      901 atcaattccg ccaggccgga cctattgccc gggtcggcgt cgatgtggtc ggcggctgcg
      961 gcgagggtgg cctcggcggc ggcgaaggcc gcgtccacag cgcccaggtg ggccagcgtg
     1021 tggtcgcccg cgcaaccgct ggcggcgcgc tcgtagagcg gcgctgctac cgcacgcgcc
     1081 ccgcccaacc aacaggccgc caccccgatg gcaccatgcc aaaagcccgg ccgcgcaagg
     1141 taatcacccg gtgcaccgat cggcatcgct ggtgcatcat caaagcttac ggatcttgtg
     1201 tcggaggtcg ccatgcccgc attgcgccac gagctagcca gcggagtaac accgggctga
     1261 gccaagtcga cagcgaacag ctcgcggttt ccgtccacac cgtgagcggt gaccagtgca
     1321 tgtgagcaca gaccggcgcc cgaacaccac ggcttcacgc ccccgagcgc cacggcatcg
     1381 ccgtcgtgac cagccgtcag cctttgtcca ggtgcctcag cggcccacac accccacagt
     1441 tgccccggtt gcgcggatgg cccacctagt tcggcgagga tcgcgaccgc gtcggtgtgg
     1501 gcttcggcca accggcctgc cacaacatcg acttctgtga attcggcgag ctttaaccac
     1561 cggccgacgg tgtcccctga cgctggtagc ggaaggttca gctcgccggc atccagccac
     1621 tgacgtacca gagacgttgt catgccagat cctcggcgcc acgtcgatgc agcatggacc
     1681 gcaggtgcgc ggcgaacccg ttgggtgccc gtccctcgtg ccgcgctgac gtgaccaccg
     1741 agaggctggc gtcgcaccgg atgtgaaagc cgcttagctc aaaccggcgc accaagtcca
     1801 catcctcgcc gctcggcaat gcggcgaaac ctcctacttc ccaatacttc tcggcagcaa
     1861 aacccatgtt cgcgccgtgg atgtggttat ggcctgcgcc atcgcttcca gatttggatc
     1921 ggtagcggtg taggtaccgg cccgcgaccg acgtcgggac atgtcgccag ttcgcgacgc
     1981 ggaccacacc gagcaccatg tccgcgtcgg cgaccatctg ccgcaccagc cagtcggggt
     2041 ccacgcggct gtcggcatcg gtggtggcat accacgtacg ggccacgttt gcgtcgggcc
     2101 acacggtccg cgcacgattg aatccggccg cacgcgccgc gccgacattg tgagcgtcga
     2161 tcgacagcac ctgcacaccc ggaccgaacc cttcggccac atcggcgctg cggtcggtac
     2221 aggcgtcgag caccaccacc gtcagtaccc gacccggcat agccgtggca gcgcccagca
     2281 cggcggtcaa gcacctccgc agcctcgtcg cctcgttgtg agcgggaatg accaccaccg
     2341 cccgatcgaa acccgctcgc gtcacgggag tgaggtaccc gttttcgcga agtttgaaca
     2401 gctagatggg tgggtatgcg cgcccgcaaa cgatgcaacg accacacgtc acacggaaag
     2461 ggagcggaag cgcatgagcg ccgaagacaa actaagcaac aagatcgacg acatgggcgg
     2521 caaggcaaaa gaagccgccg gcaagctcac cggtgacgac agcaccaaga acgaggggaa
     2581 gttggaccag gcgaaggcc
//
LOCUS       DASWBF010000454         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1092713,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000454 DASWBF010000000
VERSION     DASWBF010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092713"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VF874_16680"
     CDS             complement(<1..338)
                     /locus_tag="VF874_16680"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX9177637.1"
                     /translation="MSSTAFEQAMAAYDAVDAALDKLDALDGGALSNAERLALLQRGE
                     SWRRRLPAVEHELISELAHAPGEELGGRPVQVLADRLRIYRRDAARRIEEAADLGART
                     TLIGEPLPPKL"
     gene            complement(486..1604)
                     /locus_tag="VF874_16685"
     CDS             complement(486..1604)
                     /locus_tag="VF874_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9177638.1"
                     /translation="MAANIDIEASGLLDGLDGDARAERAELIAWLLQHGFTVEQLRGA
                     IAPMLLPARRLLGDDGTFVSAREICETHHIDLDLLQRLQRAVGLSRVDDPDARVHLRA
                     DGEAAGRAQEFVDAGIDPDQLVSVVRILADGLSHAAEVMRYTALATLLRPGATELEIA
                     QGSEALMSRVGPLLAPMVQDMLFLQLRHMMETEAVSATERAEGSALPGARDVTVAFAD
                     LVGFTRLGEAVPPEDLERLASRLADLAYGLAVPPVRLIKTIGDAVMLVCPEPVPLLDA
                     MLNLVDAADDSDLPQLRVGIASGAAVSRAGDWFGSPVNLASRVTDVARPGAVLVAEST
                     REAIGEDDRFVWSFAGARRLKGFRGDIKLYRARRAGGI"
     gene            complement(1609..2103)
                     /locus_tag="VF874_16690"
     CDS             complement(1609..2103)
                     /locus_tag="VF874_16690"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX9177639.1"
                     /translation="MPTGLPPVVDGRTRVLVLGSIPGEVSLQMQQYYAHPRNAFWCIM
                     SELIGFDVHADYPSRLQGLLSAGIGLWDTLRNCDRPGSLDSAIVRDSMEVNDFEALFA
                     EHPGITRVFFNGAKAERVFLRRVASAVDFPVDCTRLPSTSPANAAVSYSAKLRAWRAV
                     LDVG"
     gene            2136..>2297
                     /locus_tag="VF874_16695"
     CDS             2136..>2297
                     /locus_tag="VF874_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9177640.1"
                     /translation="MSCVFCEIVAGTAPAIRIYEDDDYLAILDIRPFTRGHTLVLPKR
                     HTVDLTDTPP"
BASE COUNT          451 a          825 c          670 g          351 t
ORIGIN      
        1 agcttggggg gcagcggttc gccgatgaga gtggtgcgcg cccccagatc ggcggcctcc
       61 tcgatgcgcc gggccgcatc ccggcgatag atccgcagcc gatcggccag cacctgcacc
      121 ggccgcccac ccaactcctc gcccggtgca tgggccagct cgctgatcag ctcatgctcg
      181 actgcgggca gccggcgccg ccagctttca ccgcgctgca acaacgccag ccgctcggcg
      241 ttcgacagcg cacctccgtc tagcgcatcg agcttgtcca gcgcagcgtc caccgcgtcg
      301 taggcggcca tcgcctgctc aaacgcggtc gaactcatac ccgcaatgta gcgacgccca
      361 ccgacacgat ctggcctcaa accctgccgg acaacacaat tcaggcccag ttatccacaa
      421 ccccgaatcc atccacagat ctcgacgaac agccaaatca gcgttgacaa accaaagacc
      481 acgagttaga taccgccggc gcggcgtgcc cgatacaact tgatgtctcc gcgaaagcct
      541 ttgaggcgcc gcgcacccgc gaacgaccag acgaaccggt cgtcctctcc gatcgcctca
      601 cgggtcgatt ccgccaccag caccgcaccg ggcctcgcca cgtccgtcac cctgctcgcg
      661 agattgaccg ggcttccgaa ccagtcccca gcccggctca ccgccgctcc actcgcgatg
      721 cccacccgaa gctgcggaag gtccgaatca tcggcagcgt caaccagatt cagcatcgca
      781 tcgagcaacg gaaccggttc gggacagacc agcatcaccg catcgccgat ggtcttgatc
      841 aaccgcaccg gaggaaccgc aagcccatag gcgagatcgg cgagccggct ggcgagccgc
      901 tcgagatcct ccggcggcac ggcctccccc agccgggtga acccgacgag atccgcgaac
      961 gccaccgtca cgtcgcgggc accgggaagc gccgatccct ccgcacgttc agtcgcgctg
     1021 accgcttcgg tctccatcat gtgccgcagt tgaaggaaca gcatgtcctg gaccatcggc
     1081 gccagcagcg gtcccacgcg gctcatcaac gcttccgagc cctgagcgat ttccagttct
     1141 gttgctccgg gccgaagtaa cgtagccaac gcggtgtatc tcatcacctc ggcggcgtgc
     1201 gaaagcccgt cggcgagaat gcgcaccacg ctgaccaact ggtcgggatc gattccggcg
     1261 tcgacgaatt cctgcgctcg gccagccgcc tcgccgtcgg cacgaaggtg tacgcgtgcg
     1321 tcgggatcgt cgacgcgtga caagccgacc gctcgttgca gccgctgaag aagatccaag
     1381 tcgatgtggt gtgtctcgca gatctcccgc gccgatacga acgtcccgtc atcacccaac
     1441 agccgccgag ccgggagcag catcggagca atggcaccac ggagctgttc gaccgtgaag
     1501 ccgtgctgca gtagccacgc gatcagctcg gcacgttctg cgcgcgcgtc gccgtcgagc
     1561 ccgtcgagca gacccgacgc ctcgatgtcg atgttggccg ccaccgcgtc aacctacatc
     1621 gagcacggca cgccacgccc gcagcttcgc gctgtatgac actgcggcgt tggcgggact
     1681 cgtcgacggc agccgggtgc agtcgaccgg aaagtccact gctgacgcaa cccgccgcag
     1741 aaacacccgt tcagccttcg cgccgttgaa gaacacccgc gtgatgcctg ggtgctcggc
     1801 gaaaagagcc tcaaagtcgt tgacttccat gctgtctcgc acgatcgccg aatcaagact
     1861 tccggggcgg tcgcagttgc gcagcgtatc ccacaaacca atgccggccg aaagcaaacc
     1921 ctgtagacgg gacggatagt cggcgtgcac gtcgaaccct atgagctcgc tcatgatgca
     1981 ccagaacgca tttcgcggat gcgcgtagta ttgctgcatc tgcagcgaga cttcacctgg
     2041 tatggagccg agcaccagca cgcgcgtgcg gccgtcgacg acgggcggca gaccagtcgg
     2101 cacggtgcaa ccttacgaga cggtaccgtg gcgggatgtc atgcgtgttc tgtgaaatcg
     2161 tcgcgggcac ggcgcccgcg atccggatct atgaagacga cgattacctg gcgatcctcg
     2221 acatccgccc gttcaccaga gggcacacgc tggtcctacc aaagcggcac accgtggacc
     2281 tgaccgacac gccgccg
//
LOCUS       DASWBF010000455         6810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_516667,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000455 DASWBF010000000
VERSION     DASWBF010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516667"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /gene="manA"
                     /locus_tag="VF874_16700"
     CDS             complement(<1..462)
                     /gene="manA"
                     /locus_tag="VF874_16700"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEX9177641.1"
                     /translation="MHLLRGAVRTYAWGSRTAIAEFTGRPTPTPHPEAELWFGAHPGD
                     PAWLQTEAGEQSLLDTLTANPEGQLGTAVLDRFGECLPFLVKVLAADEPLSLQAHPSA
                     QQAAEGFDREERRGIPVTSPTRNYRDRSHKPELVIALEQFEALAGFRGVPRT"
     gene            complement(467..1534)
                     /locus_tag="VF874_16705"
     CDS             complement(467..1534)
                     /locus_tag="VF874_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015628781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TobH protein"
                     /protein_id="HEX9177642.1"
                     /translation="MNATRSAIDLDDVDGLVGADRDGLLRTASTSGAHVRAAAAAVDE
                     GALESLAGPQPRTVIWIAGRGTAATAGRMVSALVGQVAGSPIMVASGAPPWIGPLDVV
                     VVAGDDAGDPALVAAASMAARRGARLLVVAPFEGPLADAAAGRSAVLAPRLPLDDAFG
                     LTRYLAAGLAAVAAVDDAVRADLGALADEVDAEVLRSSAGREVFTNPAKALAGRMVSR
                     RVVLAADSSAALALAHHGSSILLRVAGTVAAAAGLADVVIALRSDVPGAVTDSVDALF
                     HDEQIDGPLPDRLRVLALTTNDERPVVAARTAGLDDVDLVGAQDVADATAGIISSDDV
                     QLAVLAVRLEMAAVYLRLIRG"
     gene            complement(1531..2919)
                     /locus_tag="VF874_16710"
     CDS             complement(1531..2919)
                     /locus_tag="VF874_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX9177643.1"
                     /translation="MPRPAADVHRVIKAYDVRGLVGDELDEPFVADVGAAFARLLRGE
                     GADTVAVGYDMRDSSPALAAAFASGVTGQGLDVVRIGLASTDQLYFASGFLDCPGAMF
                     TASHNPAAYNGIKLCRAGAKPVGRDTGLATIADNVIAGVAAFDGPAGNITDRDVLADY
                     GEFLRSLVNLAGKRPLRVAVDAGNGMAGHTAPAVLGPIDSITMEPLYFDLDGTFPNHE
                     ANPLDPANLVDLQHHVRATGADIGLAFDGDADRCFVVDERGEPVSPSAVTALVAAREL
                     SREIGATVIHNLITSRAVPELVIERGGTPVRSCVGHSYIKGLMAKTGAIFGGEHSAHY
                     YFRDFWGADSGMLAALHVLAALGEQQRPLSELMADYHRYEPSGEINFTVHDAQACIDA
                     VLKSFGTQIMSIDHLDGVTVDLGEGTWFNIRMSNTEPLLRLNVEAREKDTVDEIVEKI
                     AAEIHSQAGVSR"
     gene            complement(3018..3455)
                     /locus_tag="VF874_16715"
     CDS             complement(3018..3455)
                     /locus_tag="VF874_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HEX9177644.1"
                     /translation="MNVPRRCCRPGCPQYAVATLTFVYSDSTAVIGPLATAAEPHSWD
                     LCVSHAGRITAPRNWELVRHAGPLPTSPDEDDLVALADAVRESRDGVAIGSGGSAAFG
                     DYADLINGAPVGTNGAVLAPPSTHAAKIGRRRGHLRVLPDPPQ"
     gene            3578..4000
                     /locus_tag="VF874_16720"
     CDS             3578..4000
                     /locus_tag="VF874_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX9177645.1"
                     /translation="MRGSLLPPTVPGWRSRAERFDMAVLEAYEPIEQRWADRLSALDV
                     AVDEIPRISPKDPDSVQWPPEVVADGPIALARLISAGVDVRGNSTRARIVLFRKPIER
                     RVKDSDELADLLHELLVAQVATYLGVEPSVIDPTIDDD"
     gene            complement(4058..4345)
                     /locus_tag="VF874_16725"
     CDS             complement(4058..4345)
                     /locus_tag="VF874_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9177646.1"
                     /translation="MGTAKRAHLALVPEPFGLAPEIPDDQWQERALCAQTDPEAFFPE
                     KGGSTREAKRICMGCEVRDRCLDYALAHDERFGIWGGLSERERRRLKRGVI"
     gene            4729..5739
                     /gene="cofD"
                     /locus_tag="VF874_16730"
     CDS             4729..5739
                     /gene="cofD"
                     /locus_tag="VF874_16730"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX9177647.1"
                     /translation="MKVTVLVGGVGGARFLLGVQHLLGLGQFAAEGGSPHTLTAVVNI
                     GDDAWMHGVRVCPDLDTCMYTLGGGIDPERGWGHRNETWHAKEELANYGVQPDWFGLG
                     DRDLATHLVRSQMLRAGYPLSQVTEALCKRWSPGATLLPASDDRCETHVVVTDLADGT
                     KRAIHFQEWWVRYRAQVPTHSFAFVGADKATAGPGVVEAIESAGVVFVAPSNPVVSIG
                     AILAIPGIRAALRSTPAPVIGYSPIVSGKPLRGMADECLRVIGVPTTSEAVGAHYGAR
                     ATVGILDGWLVHEGDAADVPGVSVRSVPLLMTDPAATADMVRSGLEIAEHWPSIKSGP
                     RS"
     gene            5736..>6810
                     /locus_tag="VF874_16735"
     CDS             5736..>6810
                     /locus_tag="VF874_16735"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX9177648.1"
                     /translation="MTDHGSARPVELLPVPGLPEFRPGDDLAAALAMAAPWLRDGDVV
                     VITSKVVSKCEGRIVPAPADPQARDDLRRKLIDAEAVRVLAHKGKTLITENALGLVQA
                     AAGVDGSNVGATELALLPVDPDGSAATLRARLRELLGVTVGVLITDTMGRAWRTGQTD
                     VAIGAAGVEVLHDYAGAHDAHGNELVVTEVAVADEIASAADLVKGKLTGVPVAVVRGL
                     PITDDGSTAARLVRPGEEDLFRLGTDEALAEGRRQAVLLRRSVRRFTSEPVPPQDIEG
                     AVADALTAPAPHHTRPVRFVWLRDADRRTALLDRMRARWREDLSQDGKSESSIDARVA
                     RGQILYDAPELIIPVLVPDGAHDY"
BASE COUNT         1066 a         2336 c         2371 g         1037 t
ORIGIN      
        1 ggtgcgcggc acgccgcgga agccggccag cgcctcgaac tgctccagcg cgatcaccaa
       61 ctccggcttg tggctgcggt cgcggtagtt gcgggtgggc gacgtcaccg ggatgccgcg
      121 ccgctcctcg cggtcgaacc cctccgcggc ctgctgcgcg ctcggatgcg cttgcagtga
      181 cagcggctcg tcggcggcga gcactttgac cagaaagggc aggcattccc cgaaccggtc
      241 gaggaccgcg gtgccgagct ggccctcggg attggcggtc agtgtgtcca gcagcgactg
      301 ctcgccggcc tcggtttgca gccaggccgg atcgccgggg tgcgcgccga accacagttc
      361 ggcctcggga tgcggtgtgg gcgttggtct tccagtgaat tccgcgatcg ccgtgcgtga
      421 cccccacgcg taagtgcgca ccgcgccgcg taacaaatgc acgtcgctaa ccccgtatca
      481 atcgcagata caccgcggcc atttccagtc gcaccgccag cacggccagt tgcacgtcgt
      541 cactgctgat gatgcccgcg gtggcgtcgg ctacatcttg tgccccgacc agatcgacgt
      601 cgtcaagacc ggcggtccgc gccgccacca ccggtcgttc gtcgttggtc gtcaatgcca
      661 gcacccgcag ccggtccggc agcggtccgt cgatctgttc gtcgtgaaac agcgcatcca
      721 ccgaatcggt caccgcgccc ggcacatccg accgcagcgc gatcaccaca tcggccagcc
      781 cggccgctgc ggcgacggtg cccgccaccc gcaacaggat cgagctgccg tggtgcgcca
      841 acgcaagtgc ggcggaggag tcggcggcca gcaccacgcg ccgggagacc atccggcccg
      901 ccagcgcctt ggccgggttc gtgaacacct cgcgtcccgc actgctgcgc agcacttccg
      961 cgtcgacttc gtcggccagc gctccgagat cggcacgcac cgcgtcgtcg acggcggcga
     1021 ctgccgccag cccggccgcc agataccggg tcaggccgaa cgcgtcgtcg aggggcagcc
     1081 gcggcgccag caccgcggac cgcccggcgg ccgcatcggc cagtgggccc tcgaacggag
     1141 cgacgaccag cagccgggcc ccgcgccgcg cagccatcga ggcggccgcg acaagcgccg
     1201 gatcgcccgc atcgtcgcca gccaccacaa cgacgtcaag cggaccgatc cagggcggtg
     1261 cgccggaggc gaccatgatc ggcgaccccg cgacctgccc gacgagcgcc gacaccatgc
     1321 gccccgcggt ggcagcggtg ccacggccgg ctatccagat caccgtgcgg ggctgaggcc
     1381 cggccaacga ctccagcgcg ccctcgtcga ccgcggcggc agcggcgcga acgtgcgcgc
     1441 ccgacgtcga tgccgtgcgc agcaggccgt cacgatcggc gccgacgagg ccgtcgacgt
     1501 cgtcgaggtc gatcgcggac cgggtggcgt tcatcgcgaa acgcctgcct gagaatggat
     1561 ttcggccgcg atcttctcga cgatctcgtc gaccgtgtcc ttctcgcggg cctcgacgtt
     1621 cagccgcagc agcggctcgg tgttggacat ccggatgttg aaccaggtgc cctctccgag
     1681 gtcgacggtc acaccgtcca ggtgatcgat cgacatgatc tgcgtgccaa acgatttgag
     1741 caccgcgtcg atgcaggctt gcgcgtcgtg gacggtgaag ttgatctcac ccgacggttc
     1801 gtaccggtga tagtcggcca tcagctccga cagcggccgt tgttgctcgc ccaatgccgc
     1861 cagcacgtgc aacgcggcga gcatgcccga gtcggctccc cagaagtcgc ggaagtagta
     1921 gtgcgccgaa tgctcgccgc cgaagatcgc gccggtcttg gccatcagcc ccttgatgta
     1981 ggagtgccca acgcacgagc gcaccggggt gccgccgcgc tcgatcacca gctcgggcac
     2041 ggcgcgcgac gtgatcaggt tgtggatcac ggtcgcgccg atctcgcggg acagttcgcg
     2101 ggcggcaacc agtgcggtga cggccgacgg ggacaccggc tctccgcgct cgtcgaccac
     2161 gaagcagcgg tccgcgtcgc cgtcgaacgc caatccgatg tcggcgcccg tcgcgcggac
     2221 gtggtgctgc aagtcgacga ggttcgccgg gtccaacggg ttggcctcgt ggttcgggaa
     2281 cgtgccgtcc aggtcgaagt acaacggctc catcgtgatc gaatcgatcg ggcccaggac
     2341 cgccggagcg gtgtggccgg ccatgccgtt gcccgcgtcg acggccaccc gaagcggtcg
     2401 cttcccggcg aggttgacca gcgacctcaa gaactcgccg tagtcggcga gcacgtcgcg
     2461 gtccgtgatg ttgccggccg gtccgtcgaa agccgccacg cccgcgatca cgttgtcggc
     2521 gatcgtggcc agcccggtgt cgcggccgac cggcttcgca ccggcccggc acagcttgat
     2581 cccgttgtag gcggcggggt tgtggctggc cgtgaacatc gcgcccgggc agtcgaggaa
     2641 tccggacgcg aagtacagct ggtcggtgga cgccagcccg atgcggacga cgtcgaggcc
     2701 ctggccggtg acgccggacg cgaacgccgc ggcaagcgcc ggcgagctgt cccgcatgtc
     2761 gtacccgacg gccacggtgt cggccccttc cccgcgcagc aggcgcgcga acgccgcgcc
     2821 cacgtcggcg acgaacggct cgtcgagttc gtcaccgact aagccgcgaa cgtcataggc
     2881 cttgatcacg cggtgaacgt ccgcagcggg ccggggcatg acgacgactg actcctgtcg
     2941 agaacgcgac tgctggtggc cagcctaacc gttagccacg acggcgtttc ggcttcaccg
     3001 cacgccgagg cgtttgctca ttggggaggg tcgggcagca cccgcagatg accgcgtcgt
     3061 cggccgattt tcgcggcgtg cgtggacggg ggtgccagca cggcgccgtt ggtgccgacg
     3121 ggagcgccgt tgatcaagtc ggcgtaatcg ccgaacgctg cgctcccccc gctgccgatc
     3181 gctacgccgt ctcgactctc ccgtacggcg tcggcgagcg ccaccaggtc gtcctcgtcg
     3241 gggctggtcg gcagcgggcc ggcgtggcga accaactccc aattgcgcgg cgcggtgatc
     3301 cggccggcgt gggagacgca gaggtcccac gagtgcggtt cggccgcggt ggcaagcggc
     3361 ccgatgaccg cggtcgagtc ggagtagacg aacgtcagcg ttgcgaccgc atactgcgga
     3421 caccctggtc ggcagcaccg acggggaacg ttcacgtcgg agaggctatc gcgcccaatc
     3481 gcctgccggt gccggacacg cgcacggtcc aaggcgaaga tctccaaacg ttacgatcgc
     3541 ccccgtggcc ggtagacgcg gttcccggcg gcgagaaatg cgtggctcgc tgttgccgcc
     3601 gacggtgccc gggtggcgga gccgcgcgga gcggttcgac atggcagtgc tggaggccta
     3661 tgagccgatc gagcagcggt gggcggaccg gctgtcggcg cttgacgtcg cggtcgacga
     3721 gatcccgcgc atctcaccga aggatcccga cagcgtgcag tggccgccgg aggtggtggc
     3781 cgacggcccg attgcgctgg cccgtctgat ttctgcgggc gtggatgtcc gcggcaactc
     3841 gactcgggcg cgaatcgtgt tgtttcgcaa accgatcgag cggcgagtca aagacagcga
     3901 cgagttggcg gacctgctgc acgagttgtt ggtggctcag gtggccacgt atctgggcgt
     3961 ggagccgtcg gtcatcgacc cgacgatcga cgacgactag cttgtcccgc gagcagacga
     4021 gagaatcaga ggccgatgag cgctcgcgcg aagagaatca gatgacgccg cgcttgaggc
     4081 gacggcgctc ccgttcggag agcccgcccc agatgccgaa gcgctcgtca tgggccagtg
     4141 cgtaatccag gcacctgtca cgcacctcgc agcccatgca gatgcgcttg gcctcacgcg
     4201 tggagccgcc cttctccggg aagaacgcct ccgggtcggt ttgggcgcac agcgcccgct
     4261 cctgccattg atcgtcgggg atttctggcg ccagaccgaa aggctcgggc accagagcca
     4321 aatgggcacg tttggcggta cccattattt ctgcgctgga gggggcttgc ggcgtgctgc
     4381 ccacagcgct caagccccgg tcgcccagcc ggatcggttg cgggaaatcg atgtgctcgt
     4441 aaggcatttc gcctcctaac ctgatgatcg cagttggtgt gtggaatgtc gcccactatt
     4501 cagcagagtt cgaacaagtg atcgaatctc ggtctgcgac accggaaccg gagggccaac
     4561 cgggaatgac accaatgtga ttacacactt gtgagctggt ccggtcaagc gttagaacca
     4621 aaattcatac catctcgtga tcaaaattcg gcgcgccgtg atcgcggcgt gtcgtcccga
     4681 gtaaccgggc cgaacgactc tagcgccgaa ggcttagtgt cctttgatgt gaaagtcacc
     4741 gttctcgtgg gcggcgtcgg cggggcgcgc ttcctgctgg gtgttcagca cttgctcggg
     4801 ctgggccagt tcgccgccga gggcggaagc ccccacaccc tcacggcggt cgtcaacatt
     4861 ggcgacgacg cgtggatgca cggcgtgaga gtgtgcccgg atctcgacac ttgcatgtac
     4921 acgctcggtg gcggcatcga ccccgaacgc ggctggggcc accggaacga aacctggcac
     4981 gccaaggagg aattggccaa ctacggcgtg cagcccgact ggttcgggct cggggaccgc
     5041 gacctggcca cccacctggt gcgcagccag atgctgcggg ccggctaccc gctgtcgcag
     5101 gtgaccgagg cgctgtgcaa gcgctggtcg cccggcgcca cgctgctgcc cgccagtgac
     5161 gaccgctgcg aaacccacgt cgtcgtcacc gatttggctg acggcacgaa gcgcgcgatc
     5221 cacttccagg agtggtgggt gcggtatcgc gcacaggtac cgactcacag cttcgcgttc
     5281 gtcggcgcag ataaggccac ggccgggccg ggcgtcgtcg aggcgatcga atccgccggc
     5341 gttgtgttcg tcgcgccgtc caacccggtg gtcagcatcg gcgcgatcct ggcgattccc
     5401 ggtatccgcg ccgcgctgcg gtccactccc gccccggtca tcgggtactc cccgatcgtc
     5461 agcggaaaac cgttgcgcgg catggccgat gagtgcctgc gcgtgatcgg cgtgcccacc
     5521 acgtcggagg cggtcggggc gcactacggc gcccgcgcta ccgtcgggat cctcgacggc
     5581 tggctcgtgc acgaaggcga tgccgccgac gtccccggcg tcagcgtccg ctcggtgccg
     5641 ctgctgatga cggacccggc ggccaccgcc gatatggttc gcagcggctt agaaatagcg
     5701 gagcactggc cgtccatcaa atcgggtccc cggtcatgac cgaccacgga tccgcgcggc
     5761 ccgtcgagtt gctgccggtg cccggcctgc ccgagtttcg gcccggtgac gacttggccg
     5821 ccgcgctcgc gatggcggcg ccgtggctgc gcgacggtga cgtcgtggtc atcaccagca
     5881 aggtggtcag caagtgcgag ggccgcatcg tgccggcccc cgccgatccg caggcccgag
     5941 acgatctgcg ccgcaagctg attgatgccg aggcggtgcg ggtactggcc cacaagggca
     6001 agacgctgat caccgagaac gcgctggggc tcgtgcaggc ggcggccggg gtcgacggct
     6061 ccaacgtcgg cgccactgaa ctcgcgctgt tgccggtcga tccggacggc agcgcggcca
     6121 ccctgcgcgc gcggctgcgg gaactgctcg gcgtcaccgt cggggtgctg atcaccgaca
     6181 ccatgggccg ggcatggcgc accggccaga ctgacgtcgc gatcggcgcg gccggcgtcg
     6241 aggtcctgca cgactacgcg ggcgcgcacg acgcccacgg caacgaactg gtggtgaccg
     6301 aagtcgcggt ggccgacgaa atcgcctcgg ccgcggacct tgtgaagggc aagctgaccg
     6361 gagttcccgt cgctgtggtg cgcggtctgc cgatcaccga tgacggctcg accgcggcac
     6421 gactggtgcg gcccggcgag gaggacctgt ttcggctcgg caccgacgag gcgctcgccg
     6481 agggccgccg gcaggccgtg ctgctgcgtc gctcggtgcg gcggttcacc tccgagccgg
     6541 tgccgccgca ggacatcgag ggcgcggtcg cagacgcgct gacggcgccg gcgccgcacc
     6601 acacccggcc ggtgcggttc gtctggttgc gcgacgccga ccggcgcacg gcactgctcg
     6661 accggatgag ggcgcggtgg cgcgaagatc tcagccaaga cggaaaatcc gagtcgtcga
     6721 tagacgcccg cgtcgcacgc ggtcaaatcc tttacgacgc gccggaactg atcattccgg
     6781 ttctggtccc cgacggcgcg catgactatc
//
LOCUS       DASWBF010000456         5493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_706810,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000456 DASWBF010000000
VERSION     DASWBF010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706810"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VF874_16740"
     CDS             <1..388
                     /locus_tag="VF874_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9177649.1"
                     /translation="YLRIYDTHTSRELYLGETKRIATPAQRIVLHAKDRGCTRPGCTA
                     PGYLSEVHHVNEWAVHHRTDIDDLTFACGPCHKLLDDDGWTTRKNTDGITEWIPPPHL
                     DFGKPRTNGYWHPQRYFRLDDNDDPP"
     gene            500..3310
                     /gene="secA"
                     /locus_tag="VF874_16745"
     CDS             500..3310
                     /gene="secA"
                     /locus_tag="VF874_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX9177650.1"
                     /translation="MLNKLLRLGEGRMVKRLKGVADYVGSLSEDVEKLTDAELRAKTD
                     KFKKRYADGESLDDLLPEAFAVAREAAWRVLDQHPFDVQVMGAAALHFGNVAEMKTGE
                     GKTLTCVLPAYLNALAGKGVHVVTVNDYLAKRDSEWMGRVHRFLGLDVGVILSGMTPD
                     ERRIAYNADITYGTNNEFGFDYLRDNMAHRLEDLVQRGHSFAVVDEVDSILIDEARTP
                     LIISGPADGASNWYSEFARLAPLMEKDTHYEVDIKKRTIGVHELGVEFVEDQIGIDNL
                     YEAANSPLVSYLNNALKAKELFSRDKDYIVRDGEVLIVDEFTGRVLIGRRYNEGMHQA
                     IEAKESVEIKAENQTLATITLQNYFRLYDKLAGMTGTAQTEAAELHEIYKLGVVSIPT
                     NKPMVRGDKSDLIYKTEEAKYIAVVDDVAERYEKGQPVLIGTTSVERSEYLSRQFQKR
                     RIPHNVLNAKYHEQEAGIIAEAGRRSAITVATNMAGRGTDIVLGGNVDFLTDTRLRER
                     GLDPVETPDEYEQAWHEELQKVKDEAAAEAQDVIDAGGLYVLGTERHESRRIDNQLRG
                     RSGRQGDPGESRFYLSLGDELMRRFNGAALESLLTRMNLPDDVPIEAKMVTRAIKSAQ
                     TQVEQQNFEVRKNVLKYDEVMNQQRKVIYAERRRILEGENLKDQALQMIRDVVTAYVT
                     GATAEGYAEDWDLDQLWTALKQLYPVGIEHKSLAHRHDEMDDLSSEELLDALLEDADS
                     AYAKRETEIDGLAGEGAIRQLERTVLLSVLDRKWREHLYEMDYLKEGIGLRAMAQRDP
                     LVEYQREGYDMFIGMLDGLKEESVGFLFNVVVEAAPPPVVEPITAPEGLELRTAAARV
                     GGGTGTKELQAAVAALRAKGIDDDTDGERQLTYTGPSEDGSAQVQRRNGGEPTPAAVG
                     SGTRRERREAARKQSRGSKRRR"
     gene            complement(3315..3797)
                     /locus_tag="VF874_16750"
     CDS             complement(3315..3797)
                     /locus_tag="VF874_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9177651.1"
                     /translation="MEGNTVSVERVINAPASDIFALVADAGKHPSFDGSGTVKHTTQE
                     SMPLSKGSKFGMSMRVGLPYKTTNTVIEYEPDRRIAWQTTMLGGLVGGRIWRYELEPA
                     DGGAAGPATSSARTLVRETWDISRDKQAVILKNTGMPKQAEQGMRATLERIAELVEKR
                     "
     gene            complement(3808..4308)
                     /locus_tag="VF874_16755"
     CDS             complement(3808..4308)
                     /locus_tag="VF874_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HEX9177652.1"
                     /translation="MSHDLTVVPVADYEPPVGEPRRPPPTFGRPVRRLAAVPPLPEQP
                     SARAAAVFADAALRGVLEVVDGRRPFAQLQPLLGDGLADTVIALVRSSRAQADALRAS
                     GSSPGMLAAQLRRVRLRIVDRDGDVAEVFATYYRDGRVRAIAGRVQRAEIRGRRGWRL
                     VALQMG"
     gene            4504..>5493
                     /locus_tag="VF874_16760"
     CDS             4504..>5493
                     /locus_tag="VF874_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9177653.1"
                     /translation="MVIRLSASDASFYHLEDTSTPMYVGSLSILRRPKAGLSYETLLR
                     TVEQRLPQIPRYRQKVREVTLGLARPVWIDDPDFDITYHIRRSALPSPGSDDQLHELI
                     ARLASRPLDRSRPLWEMYLVEGLAKNRLAIYTKSHQALVNGMTALAIGHVIADRTQRP
                     PEFGEDIWIPAGEPSNFALMMGAVGEWVTRPTTQLQAMRSAVSDVVTNAGYIADVGRQ
                     LSHTARTVLRGTAPDSPLNTQVSRNRRFTVATGRLEEYRKLRARYECDVNDVVLAVVA
                     GALRNWLMSRGESVTTASTVRAMAPMSVYPEADLETNAGPGQAISEVSPFLVDL"
BASE COUNT         1017 a         1839 c         1839 g          798 t
ORIGIN      
        1 ctatctgcgc atctacgaca cgcacaccag ccgcgagctg tatctcgggg aaaccaaacg
       61 catcgccaca ccggctcagc ggattgtgtt gcacgccaaa gaccgtggct gcacacgacc
      121 cgggtgcacg gcgccgggct acctgtcaga ggtccatcac gtcaacgaat gggccgtgca
      181 ccaccgcacc gacatcgacg acctgacatt cgcctgcgga ccgtgtcaca agctgctcga
      241 cgacgacggc tggaccaccc gcaagaacac cgacggcatc accgaatgga tcccgccgcc
      301 ccacctggat ttcggcaaac ccagaaccaa cggctactgg cacccccaac gctacttccg
      361 actcgacgac aacgacgacc ccccgtagcg gctgaccgca gctccttcgt cctcacgacg
      421 cggaggtttg acctctggtg ttggcgtgtc gcctaccatg ggggacgccg aagattccaa
      481 ataattaggg gacgtatccg tgctgaacaa gttgctgcgc ctcggtgaag gtcgcatggt
      541 caaacgcctc aagggcgtgg ccgactatgt cggcagcttg tccgaggacg tcgagaagct
      601 caccgacgcc gagttgcgcg ccaagaccga caagttcaag aagcgctacg ccgacggcga
      661 aagcctcgac gacctgctgc ccgaggcgtt cgcggtggcc cgcgaagccg cctggcgggt
      721 gctcgaccaa catccgttcg acgtgcaggt gatgggcgcc gcggctctgc acttcggcaa
      781 tgtcgccgag atgaagaccg gtgagggcaa gaccctcacc tgtgtgctgc ccgcctacct
      841 caacgcgctg gccggcaagg gcgtgcacgt cgtcaccgtc aacgactacc tggccaaacg
      901 cgacagcgag tggatgggcc gcgtgcaccg cttccttggt ctcgacgtcg gcgtgatcct
      961 gtccggcatg actcccgatg agcggcgcat cgcctacaac gccgacatca cctacggcac
     1021 caacaacgaa tttgggtttg actatttgcg cgacaacatg gcccaccggc tcgaagatct
     1081 ggtgcagcgc gggcacagct tcgcggtcgt cgacgaggtg gactcgatcc tgatcgacga
     1141 ggcccgcacc ccgctgatca tctccggacc cgccgacggt gcgtcgaatt ggtacagcga
     1201 gttcgctcgg ctcgcgccgc tgatggagaa ggacacccac tacgaggtgg acatcaagaa
     1261 gcgcaccatc ggcgtgcacg agctcggggt cgagttcgtc gaggaccaga tcggtatcga
     1321 caacctctac gaggccgcca actcgccgct ggtcagctac ctcaacaacg cactgaaggc
     1381 caaggagctg ttcagccgcg acaaggacta catcgtccgc gacggcgagg tgctgatcgt
     1441 cgacgagttc accgggcgtg tgctgatcgg gcgccgttac aacgagggca tgcaccaggc
     1501 catcgaggcc aaggaaagcg tggagatcaa ggccgagaac cagacgctgg ccacgatcac
     1561 cctgcagaac tacttccggc tctacgacaa gctcgcaggc atgaccggca ccgcgcagac
     1621 cgaggcggcc gagttgcacg agatctacaa gctcggcgtc gtgtcgatcc cgaccaacaa
     1681 gccgatggtc cgcggcgaca agtccgacct catctacaag accgaggaag ccaagtacat
     1741 cgcggtcgtc gacgacgtcg ccgagcgcta cgagaagggc cagccggtgc tgatcggcac
     1801 caccagcgtc gagcgctccg agtacctgtc ccgtcagttc cagaagcggc gcatcccgca
     1861 caacgtgctc aacgccaagt accacgagca ggaggcgggc atcatcgccg aggccggccg
     1921 tcgcagcgcg atcaccgtcg cgacgaacat ggccggccgc ggcaccgaca tcgtgctcgg
     1981 cggcaacgtc gacttcctca ccgacacgcg gctgcgcgag cgagggctcg acccggtcga
     2041 gacacccgac gagtacgagc aggcgtggca cgaggagctg caaaaggtca aggacgaagc
     2101 cgccgccgag gctcaagacg tgatcgacgc cggcggcctg tacgtgctcg gcaccgagcg
     2161 gcacgagtcg cggcgcatcg acaaccagtt gcgcggccgg tcgggccggc agggcgaccc
     2221 aggggagtcc cggttctacc tgtcgctcgg cgacgagttg atgcggcggt tcaacggcgc
     2281 ggcgttggag tcgctgctca cccggatgaa tctgccggac gacgtgccga tcgaggccaa
     2341 gatggtgacc cgcgcgatca agagcgcgca gacccaggtc gagcaacaga acttcgaggt
     2401 ccgcaagaac gtcctaaagt acgacgaggt gatgaaccag cagcgcaagg tcatctacgc
     2461 cgagcgcagg cggatcctcg agggcgaaaa cctcaaagac caagcgctgc aaatgattcg
     2521 ggatgtggtc accgcctacg tcaccggcgc caccgcggag ggctacgccg aggactggga
     2581 tctcgaccag ctgtggaccg cgctgaagca gctgtatccg gtcggcatcg agcacaagag
     2641 cctggcccat cgccacgacg aaatggacga cctcagcagt gaggaactgc tcgacgcgct
     2701 gctggaggac gccgacagcg cgtatgcaaa gcgggagacc gagatcgacg ggctcgccgg
     2761 cgagggcgcg attcgccagc tcgaacgtac cgtgctgctc agtgtgctcg accgcaagtg
     2821 gcgtgagcac ctctacgaga tggattacct caaggagggc attggcctgc gggcgatggc
     2881 gcagcgtgac ccgctcgtcg aataccagcg cgagggctac gacatgttca tcggcatgct
     2941 cgacgggctg aaagaggagt ccgtcggatt cctgttcaac gtcgtggtcg aagcggcgcc
     3001 gcccccggtc gtcgagccga tcaccgctcc cgaggggctc gaactgagga ccgccgccgc
     3061 ccgggtcggt gggggcacgg gcaccaagga gctacaggcg gccgtggcgg cgttgcgcgc
     3121 caagggaatt gacgacgaca cggatggcga gcgacagctc acctacaccg ggccgtcgga
     3181 ggacgggtcg gcgcaggtgc agcggcgcaa cggcggcgag ccgacgcccg cggccgtcgg
     3241 cagtggcact cggcgcgaac ggcgcgaggc cgcccgcaag cagagccgcg ggtccaaacg
     3301 aaggcgctag ggcgtcaacg cttttcgacg agctcggcga tgcgctccag cgtcgcgcgc
     3361 atgccctgct cggcctgttt gggcatgccg gtgttcttca gaatcaccgc ctgcttgtcc
     3421 cgcgagatgt cccaggtctc gcgcaccagc gtgcgggcgc tggatgtcgc cggcccagcg
     3481 gctccgccgt ccgcgggctc cagctcgtat cgccagatcc ggccgccgac caagccgccc
     3541 agcatcgtcg tctgccatgc gatgcgccgg tccggttcgt actcgatgac cgtgttggtg
     3601 gtcttgtagg gcaagcccac ccgcatcgac atcccgaact tcgagccttt cgacagtggc
     3661 atcgactcct gcgtcgtgtg cttgacggtg ccggagccgt cgaagctggg atgcttcccg
     3721 gcgtcggcga cgagtgcgaa gatgtcgctg gcgggggcgt tgatgactcg ttcgacgctt
     3781 accgtgttcc cttccacggg gttgacgcta gcccatctga agcgcaacca gtcgccatcc
     3841 ccgacgaccg cggatctcgg cgcgctggac ccgtcccgcg atagcccgca cgcgcccgtc
     3901 gcggtaatac gtggcgaaca cctcggcgac gtcgccatcg cggtccacga tgcgcagccg
     3961 gactcgtcgc agttgcgccg ccaacattcc cggcgatgag ccgcttgcgc gaagagcatc
     4021 agcctgagcg cgcgacgagc ggaccagcgc gatcaccgtg tcggcgagcc cgtcaccgag
     4081 cagcggttgc agctgggcga acggccggcg tccgtcgacg acttcgagca ccccccgcaa
     4141 cgccgcgtcg gcgaacaccg ccgcagcgcg cgccgacggc tgttcgggca gcggcgggac
     4201 cgcggcaagc cgccggacgg gtcggccaaa ggttggtggc gggcgccgcg gctcaccgac
     4261 cggcggctcg tagtcggcga cggggacgac ggtcaagtcg tggctcatgg gcgccctcca
     4321 gcggtgacgg ttccgacgtg cctgctggag agacgcagac ctgctgcgac caatgatccc
     4381 acggcaaacg aggggtcccg tcatgaaatt gcgctgcggc cacccacaat gcgagggtgc
     4441 ccgagcgccg gatacggtgg cggaagctgc ggctgagttc gagcgagggg aggttcggcc
     4501 gcgatggtga tccggttgtc ggcgtctgac gcgtcgttct accacttgga ggacacatcg
     4561 acgccgatgt acgtcgggtc gctgtcgatc ctgcgccgac cgaaggcggg cctttcgtac
     4621 gagacgctgc tgcgcaccgt cgagcagcgg ctgccgcaga tcccgcgcta ccggcagaaa
     4681 gtgcgcgagg tgaccctcgg gctggcccgg ccggtgtgga tcgacgaccc cgacttcgac
     4741 atcacctatc acatccggcg ctccgcgctg ccgtccccgg gcagcgacga ccagctgcac
     4801 gagctgatcg cgcggctggc gtcgcgaccg ctggaccggt cgcggccgct gtgggagatg
     4861 tacctggtgg aagggctcgc caagaaccgc ctcgcgatct acacgaagtc ccaccaagcg
     4921 ctggtcaacg gaatgacggc gctggcgatc gggcacgtga tcgccgaccg gacgcagcgc
     4981 ccgcccgagt tcggcgagga catctggatc ccggcgggcg aaccgtcgaa cttcgcgctg
     5041 atgatgggcg ccgtcgggga atgggtgacg cggcccacca cgcaactgca ggcgatgcgg
     5101 tccgcggtca gcgacgtcgt caccaatgcc ggctacatcg ccgacgtggg ccgtcagctg
     5161 tcccacacgg cccgcaccgt gctgcgcggc accgccccgg acagcccgct gaacacccag
     5221 gtgtcgcgca accgccggtt caccgtggcc accggccggc tggaggagta ccgcaagctg
     5281 cgggctcgct acgagtgtga cgtcaacgac gtcgtgctgg cggtggtcgc gggggcgctg
     5341 cgcaactggc tgatgtcgcg gggcgagtcg gtgacgacgg cgtcgaccgt gcgggcgatg
     5401 gccccgatgt cggtgtaccc ggaggccgac ctggaaacca acgcgggtcc cggtcaggcg
     5461 atcagcgagg tgtcgccgtt cctggtggac ctg
//
LOCUS       DASWBF010000457         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_706876,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000457 DASWBF010000000
VERSION     DASWBF010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706876"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..495
                     /locus_tag="VF874_16765"
     CDS             208..495
                     /locus_tag="VF874_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9177654.1"
                     /translation="MPRRKQVYKITYPNGKIYVGMDLTGTLLYMGSPSAHEQIAADLG
                     LEPHRFDVTVRKEILWESETATDAEVRAMEVKLIRETGANADFHAAPYPSG"
     gene            714..2069
                     /locus_tag="VF874_16770"
     CDS             714..2069
                     /locus_tag="VF874_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9177655.1"
                     /translation="MKQQPEEIPDAEHELVIERVAAIDVAKASGKVCVRLPRPGGQVG
                     RLSRVWDVAATTRAVTELAEHLIGERIEKVTVESTSDYWRIWFYLLEAAGLAVQLVNA
                     RDVKNVPGRAKSDKLDAVWLAKLTEKGMLRASFVPPAPIRELRDYTRLRIDLTHDRSR
                     HVQRLEKLLEDALIKLSAVASDIMGVSGRAMIEALIAGERDPRRLADLARGKMKAKRA
                     ALIEALTGRFDAHHAELARMLLDQIDALTGQIDTLTTRIETLIEAIPAAHGVDPDGTT
                     GPHAGTGPDAVVLPAVARLDEIPGIGPRAAQIILAEIGTDMSRFPTAGHLVSWAKLCP
                     RTIQSGPVTRGGKTGKGNPYLKGALGEAAAAAAKTDTFIGERYRRLVKRRGKLKALVA
                     VARSILVIVWQLLTDITARFHDLGSDYHTRRIGTEKRIRSHVAQLTAMGYQVTLTPAA
                     A"
     gene            2403..>3375
                     /locus_tag="VF874_16775"
     CDS             2403..>3375
                     /locus_tag="VF874_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9177656.1"
                     /translation="MELLHERCAALDIGKKELKACVRAPSPKGKRSRRQEIRTYATTT
                     NALLELRDWLVAEQVTLVVLEATGDYWRGAFYLLEDDLNVILVNAAHAKGLPGRKTDV
                     CDASWLCQMGECGLLRASFVPPEPIRQLRDLTRYRTTLTAERSREAQRLEKELEDAGI
                     KLSTVATDILGVSGREMLAALIDGERDAQVLAGMAKARMRPKIPQLVQALTGNFGEHH
                     AFLCRLHLQRIDQLSTTIEELSTRIEEQMRPFSRQLERLATIPGVGQTVAEVIVAVTG
                     ADMSRFRTAAHLVSGTGVCPGHHESAGKRKSGKTRHGNRWLSGALGTA"
BASE COUNT          611 a         1129 c         1133 g          502 t
ORIGIN      
        1 agctggaggc cgaggcgaag gccaacccga agcgcagcgc gctgctcgag ccggcgggac
       61 gggccggcgg accagtgtca gtactgaatt ccggcgtctc agttcgcttc taatccacga
      121 tcgcgttagc tcgcgggtga tcccgcgtta gctcgcgggt gatccccgta tgggcggacc
      181 gccgcgcgac gatggcacaa tccggccatg ccccgacgca agcaggtcta caagatcacg
      241 taccccaacg ggaagatcta cgtcggtatg gacctcacgg ggacgctgct gtacatgggc
      301 agcccgagcg cccacgagca gatcgccgct gacctcggac tcgaaccgca ccggttcgat
      361 gtgacggtgc gcaaagaaat cctgtgggaa tccgaaacgg ccaccgacgc ggaggtccgc
      421 gcgatggaag tgaagcttat ccgggagacg ggcgctaacg cggatttcca cgcagccccc
      481 tatccctccg ggtgacgcct cgcaggcgtg aaaatggggt cgggtcgcct gccgacgccg
      541 acacctactt gggtatgcga atccgacact gcagcctggc gccggggccc gacctggaag
      601 ggtcacgcat tgttgcttcg agcacgccgg tcagaatttc ggtttccctt gaccgaggcc
      661 cccgggcggc ccgccctttg gaagtaagcg gatagtactg gaggacaaac gaattgaagc
      721 aacaaccgga agagattccc gacgccgagc acgagctggt catcgaacgg gtcgccgcga
      781 tcgatgtggc caaggcgtcg ggcaaggtgt gtgtgcgcct accgcgaccc ggtgggcagg
      841 tcgggcggct gagccgggtg tgggacgtgg cggcgaccac tcgggcggtc accgagttgg
      901 ccgagcacct gatcggcgag cggatcgaga aggtcaccgt cgagtcgacc tcggactact
      961 ggcggatctg gttctacttg ctggaggcgg ccgggctggc ggtgcagctg gtcaacgccc
     1021 gcgatgtcaa gaacgtgccc ggccgggcca aaagcgacaa gctggacgcg gtgtggctgg
     1081 ccaagctgac cgagaagggg atgctgcggg cgtcgttcgt gcctccggca ccgatccggg
     1141 aactgcggga ctacacccgg ttgcggatcg atctgaccca cgaccgttcc cggcacgtgc
     1201 agcggctgga gaaactgctc gaggacgcgt tgatcaaact gtcggcggtg gccagcgaca
     1261 tcatgggcgt ctccggccgc gcgatgatcg aagcgttgat cgccggcgag cgcgacccgc
     1321 gccggctcgc cgacctggcc cgcggaaaga tgaaagccaa acgggccgcc ctgatcgagg
     1381 cgttgaccgg ccggttcgat gctcaccacg ccgaactcgc gcggatgctg ctcgaccaga
     1441 tcgacgccct caccggccag atcgacaccc tcaccaccag gatcgagacc ctgatcgagg
     1501 ccatcccggc cgcacacggc gtcgacccgg acggcaccac cggcccgcac gcgggcaccg
     1561 gcccggacgc ggtcgtcctg cccgcggtcg cgcggctcga cgagatcccc ggcatcgggc
     1621 cacgcgccgc gcagatcatc ctcgccgaga tcggcacgga catgagtagg ttccccaccg
     1681 ccgggcacct ggtctcgtgg gcgaaactgt gcccgcgcac catccagtcc ggcccggtaa
     1741 cgcgcggcgg caagaccggt aagggcaacc cctacctgaa aggcgcgctc ggcgaggcgg
     1801 ccgccgcggc cgccaagacc gacaccttca tcggcgaacg ctaccggcgc ctggtcaaac
     1861 gccgcggcaa actcaaagcc ctggtcgccg tggcacgttc catcctggtc atcgtatggc
     1921 agctgctgac cgacatcacc gcccgcttcc acgacctggg cagcgactac cacacccgcc
     1981 gcatcggcac cgaaaaacgg atccgcagcc acgtcgccca gctcaccgcg atgggctacc
     2041 aggtcaccct cacacccgcc gccgcctgag ccgaaacacc acccatatcg acaagtcaac
     2101 gccaagccgg ctccgcaagc tccgccggct cccctcgccc tgcccactca cccaagtcat
     2161 tttccggtca ggaacccagg cccgctgcgc tgggccgccg tggaggcggg aaaatggcgt
     2221 cgcgttcgtc cgggacgccg aatctactgt gatgccctga gctcgtcggt caggatggtg
     2281 tggggagcca ccgacaggaa ctgtcgattg ttgcggtaag cacgcggacc agattcttta
     2341 ggccctgtgg ttcctccgga cggacgcgca atgttcgaag ttgttgtgtc cggagggatt
     2401 tgatggaact gttgcatgag cggtgcgccg cgttggatat cggcaagaag gagctgaagg
     2461 cgtgcgtgcg ggcgccgagt ccgaagggca agcggtcgcg ccgccaggag atccgcacct
     2521 atgcgaccac caccaatgcg ctgctcgagc tgcgggattg gctggtcgcc gaacaggtca
     2581 ccctggtggt gctcgaggcg accggcgact actggcgtgg cgcgttctac ctgctggagg
     2641 acgacctgaa cgtgatcctg gtcaacgccg cgcacgccaa gggtctgccc ggtcgcaaaa
     2701 cggacgtctg cgacgcctct tggttgtgtc agatgggtga gtgcgggctg ctgcgggcgt
     2761 cgttcgtgcc gccggagccg atccggcagc tgcgggatct gacccgctac cgcaccacgc
     2821 tgaccgccga gcgcagccgg gaggcgcagc ggttggagaa ggagctcgag gacgccggga
     2881 tcaagctgtc gacggtggcg accgacatcc tcggtgtgtc gggacgggag atgctggccg
     2941 cgttgatcga cggcgaacgc gacgcgcagg tgctggccgg gatggccaag gccaggatgc
     3001 ggccgaagat cccgcaactg gtgcaggcgt tgaccggcaa cttcggcgaa caccacgcgt
     3061 ttctgtgccg gctgcacctg cagcgtatcg accaactgtc caccacgatc gaggaactct
     3121 cgacgcggat cgaagagcag atgcgccctt tctcccgcca acttgaacgg ctggcgacca
     3181 tccccggcgt cggccagacc gtggccgagg tgatcgtggc cgtgaccggt gccgacatga
     3241 gccgcttccg caccgccgca cacctcgtct ccgggaccgg agtgtgcccg ggccaccacg
     3301 aatccgccgg caaacgcaaa tcgggcaaga ctcgtcacgg caaccggtgg ttgtccggcg
     3361 cgttgggcac cgccg
//
LOCUS       DASWBF010000458         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1161428,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000458 DASWBF010000000
VERSION     DASWBF010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161428"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..450
                     /locus_tag="VF874_16780"
                     /pseudo
     CDS             229..450
                     /locus_tag="VF874_16780"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(586..1380)
                     /gene="hypB"
                     /locus_tag="VF874_16785"
     CDS             complement(586..1380)
                     /gene="hypB"
                     /locus_tag="VF874_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX9177657.1"
                     /translation="MGRFHRHDDGTVHAHEHGEDEHSHHHDHAHEAGDHSGYDTGSQR
                     VDVLEQIFAENDIRADVNRQAFETNGIRALNLMSSPGSGKTTVLGVTLDEFAGDIAVG
                     VIEGDIATDLDAAKLGGRGAQVSLLNTSNGFGGECHLDAPMVNRALQGLDLSGLDLVI
                     IENVGNLVCPAEFDVGEHAKAMVYSVTEGEDKPLKYPVMFRSVDVVLLNKIDLVPHLD
                     VDVDTYIANVRQVNPAATILPVSARTGAGMGAWFDWLRRFVGEAST"
     gene            complement(1393..1728)
                     /locus_tag="VF874_16790"
     CDS             complement(1393..1728)
                     /locus_tag="VF874_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX9177658.1"
                     /translation="MHELSLCDAIATVVRPYAEGRHVDIVRVRIGALRQVVPDSLSFC
                     WTLVREHEGMPDAELELELVAAEVVCRSCGRQSEITSRWSILCPKCASEDVEVLRGEE
                     FLVTSLDVS"
     gene            2139..2432
                     /locus_tag="VF874_16795"
                     /pseudo
     CDS             2139..2432
                     /locus_tag="VF874_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2380..2962
                     /locus_tag="VF874_16800"
                     /pseudo
     CDS             2380..2962
                     /locus_tag="VF874_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017207035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(3080..3523)
                     /locus_tag="VF874_16805"
     CDS             complement(3080..3523)
                     /locus_tag="VF874_16805"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX9177659.1"
                     /translation="MFEFTERARRVVGQAYEEANAHCNDYVGTEHLLLGLIHDRHDAV
                     VEMLQVLGLGAEAIRRQVEETIGPPAQRPPPAHLPFSPQVKNAVKQSWHEAQLRGHDL
                     VDAEDILCGLTRQTDCAAAQALADLGIEPARIANRCRCYPQAGLA"
     gene            3718..3846
                     /locus_tag="VF874_16810"
     CDS             3718..3846
                     /locus_tag="VF874_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177660.1"
                     /translation="MSLSTGLAAPQAVVAAATVVASAEPKLYGTQRKAKPSAALAR"
BASE COUNT          682 a         1332 c         1233 g          689 t
ORIGIN      
        1 ggggacgatc cctcgggtcc gcgcgggccc gtagccgacg ttctgcgcct ctccgtcggt
       61 tgtcggcact ggtcgccgcc ggcctgttac tggtcgctgt gtccgacctg cggaactggc
      121 cggtgcccga ccggtgaaga acgctaagcg tcgcgtggtg ccggtgactg cgggcttggt
      181 gaatctgtat gagcggtatc gggtcgatcg cgactctgca ggcaggcccg cgaatccgat
      241 tttgtgttcg tgaatctgta tcgcgaaccg ttcggggagc cgatgaaact gcatgccgcg
      301 aacgcgctcc tgcgccggct gagccgccgc accgggcatg cggttactcc gcacatgctg
      361 cgacacacgt ttggaacggg cgcggcgcag gccgtgtcgt tggacgttgt cgccgaactt
      421 tggcccggct gcagcccgtc tgccgcgtag tgaccacttg ctagcaggtg gtatctgacg
      481 ttggcgaggt gaagaccgta ggccgacgtg tccctatacc cgtcctgaat cgtcgatgga
      541 cttcagccaa gtgtcaggaa caatcgccga cggcggcgta tcaggtcagg tcgacgcttc
      601 cccgacgaag cggcgcagcc agtcgaacca ggcgcccatg ccggcaccgg tgcgggcact
      661 cacgggcagg atcgtcgcgg cggggttgac ctggcgaacg ttggcgatgt aggtgtcgac
      721 gtcaacatcc aaatgcggca ccaggtcgat cttgttgagc agcaccacat ccaccgaacg
      781 gaacatgacg ggatacttca gcggcttgtc ctcgccctcg gtgacggagt agaccatcgc
      841 cttggcgtgt tcgcccacgt cgaactctgc cgggcacacc aggttcccga cgttctcgat
      901 gatgacgagg tccagtccgg acaggtccag accctgcagg gcacggttga ccatcggggc
      961 atcgagatgg cactcgccgc cgaagccgtt gctggtgttc agcaacgaca cctgcgcgcc
     1021 gcggccaccg agtttggccg catccagatc ggtggcgata tcgccctcga tcacccccac
     1081 ggcgatatca cccgcgaatt catcgagggt gacccccagc accgtggtct tccccgaacc
     1141 aggcgaactc atcagattca gcgcccggat tccattggtc tcgaacgcct gccgattgac
     1201 gtcggcccga atatcgttct cggcgaaaat ctgttccagg acatcgacac gctgagagcc
     1261 ggtgtcatag ccgctgtggt cgccagcctc atgagcgtgg tcgtggtggt gtgaatgctc
     1321 gtcctcacca tgctcgtggg cgtgcacggt cccgtcgtcg tgccggtgaa atctacccat
     1381 gcccaacacc tttcatgaaa cgtccaacga ggtcaccaga aactcctcgc cgcgtagcac
     1441 ctcgacgtcc tcgctggcgc acttcgggca cagtatcgac caccgggagg tgatttcgga
     1501 ctgtcgccca catgaacggc acaccacctc cgcggcgacg agttcgagct ccagctcggc
     1561 gtcgggcatc ccctcatgct cacggacgag ggtccagcag aacgacagcg agtcaggcac
     1621 cacctgacgc agggccccaa tccgcacacg cacgatgtcg acgtggcggc cctccgcata
     1681 cggtctgacc acagtggcga tcgcatcaca caacgacagc tcatgcacgg cccgtcaccg
     1741 tcacctcacc tcgttaggca cgctgctggc acaccgaagt ggaagtgcga ggttatcact
     1801 gcccagcagg ctcatgagca cctttgtacc ctcggcgagc gtccggtgaa ctgctggccg
     1861 gcccctgcga acgtgagggt ggccctcgac gtcatccagc gtcacgacca accaacccgg
     1921 acatgaaact cgtcggacaa cttccatgga tccggtcgcc tcgcgggcac agattgcggt
     1981 gcaccacacc gcggttggcg gtgcttgcgg ccctgatgac cgatcccgac gccgggcatc
     2041 gcagcgccgc gcagattcac cggcgactcg tcgcggacgg gcgcgggttg gatctggcca
     2101 gcgtgccgac gaccgtggac ggcatcgatc aggagccgat ggacggcacg agtttcgtgc
     2161 acaccttcga cgacgacgcc gccgccgccg ccgccgagga gcgtcacacg gtaaccagga
     2221 gccgacttct tcggttctcc gcgaggcaca cacccgccca tcccgagttc tcaaacactc
     2281 tctcagtgcc aagcaagcac gccagcttgc tgtgctgctc gtcgaggcgg ccgacgaagt
     2341 cgaccggccg caggcagttg cttcaggagg tggggggata tgacttcttc ggcggccccc
     2401 gcaccgttga cgtgcacgtg cgccggctac gacaaccccg tcgggcgcgc cgaagtgaaa
     2461 tttgccgcgg ttagtgttgc cggggtcgac ccgagtcggg tattcactga caaactctcc
     2521 gggtcagcca acaccgcccg gccgggcctc accgcgatgc tggactatgc gcggcccggc
     2581 gacaccgttg tcgtcgccgc aattgaccgg ctcggccgat ccgtcgccga agtcacccgg
     2641 accaatcgcc gatcttgacg agcggcgaat cacgttgcgc gccttacgtg aaggcgtcga
     2701 taccgctacc ccgacagggc gggccgtcgc agctatcatg gccaccctcg ccgaactcga
     2761 gctcgaactc ggccgggaac gccgcgccgc atcccgccaa tcccgtcggg tgagaggcct
     2821 gccggcgacg aagccatgca agctcgggcc agatcgtcaa gaacagttac ggcgtctcgc
     2881 cgcgacggga gagcccgtct gtgagctggc cgcggctttt gggatcgggc gggccactgc
     2941 gtaccgctac ttatctcagt agtcctcccg gccacttccc cggccatgca ccacgccgcg
     3001 atcatcccga aacctgcttg gtctcaaccg gtcagcgcat cacttgcgcg acgtagtcgg
     3061 ggccgccgac tcacgccgtt taagcaaggc cggcttgggg atagcagcga cacctgttgg
     3121 cgatccgcgc tggctcgatc ccgagatccg cgagggcttg ggccgcggcg cagtcggtct
     3181 ggcgggtcag gccgcacaga atgtcctcgg cgtcgacgag gtcatggccg cgcagctgcg
     3241 cctcatgcca gctctgcttt accgcgtttt tgacttgcgg gctaaacggc agatgagcgg
     3301 ggggaggtcg ttgggccggc ggcccgatcg tctcctccac ctgccggcga atcgcctcgg
     3361 cgcccaggcc tagaacctgc agcatctcca ccaccgcatc atggcggtca tggattagcc
     3421 cgagcagcag atgctcggtc ccgacgtagt cgttgcaatg cgcattggct tcttcatagg
     3481 cctgcccgac gacgcggcgc gcacgctcgg tgaattcgaa caccccggtc ctctcccctg
     3541 cgggcggcac ttgctttgat ggtgaagcag ctttccacac caggacgtcc aacgacgtcc
     3601 aacgttcgat ttcgggcttc atcgcggcgg cgggctgtcg tctgacagtt gattccaggg
     3661 atgcggactg tccaccagcg tccggcacag caactccaca gcacgaagta ggacgccatg
     3721 tccctgtcga ccgggctggc cgcaccccag gctgtggtgg cggctgcgac ggtggttgcc
     3781 agcgccgagc cgaaactata tgggacacag cgcaaggcga agccttccgc cgcgctggcc
     3841 cgctgactag cactagcgtt gagtcatgca gtacatggat cggcgcgcga tgctgtgcgc
     3901 catcgcggtc accgtgctgg cggccgccac ccgggc
//
LOCUS       DASWBF010000459         7806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1030638,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000459 DASWBF010000000
VERSION     DASWBF010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030638"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="VF874_16815"
     CDS             <1..1739
                     /locus_tag="VF874_16815"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9177661.1"
                     /translation="PRATRHLFVTGGVASSLGKGLTASSLGQLLTSRGLRVTMQKLDP
                     YLNVDPGTMNPFQHGEVFVTEDGAETDLDVGHYERFLDRNLSAQANVTTGQVYSSVIA
                     KERRGEYLGDTVQVIPHITDEIKSRILAMAQPDADGNRPDVVITEIGGTVGDIESQPF
                     LEAARQVRHDVGRDNVFFLHVSLVPYLAPSGELKTKPTQHSVAALRSIGIQPDALILR
                     CDRDVPEPLKNKIALMCDVDIDGVISTPDAPSIYDIPKVLHREELDAFVVRRLHLPFR
                     DVVWTEWDDLLRRVHEPGETVRIALVGKYVDLSDAYLSVAEALRAGGFKHWAKVEMRW
                     VASDDCQTDSGAVAALADVHGVLIPGGFGIRGIEGKIGAVRHARTHGLPVLGLCLGLQ
                     CIVIEAARSVGLTQANSAEFDPQTPDPVISTMADQEHIVAGQADLGGTMRLGAYPAVL
                     EPDSVVAEAYQATEVSERHRHRYEVNNDYRDQIAASGLRFSGTSPDGRLVEFVEYPRE
                     VNPFLVGTQAHPELKSRPTRPHPLFVSFIGAALDYKAAERLPVEIPEQRPNGNEHPAG
                     VDGLLHQQVARG"
     gene            1732..2355
                     /locus_tag="VF874_16820"
     CDS             1732..2355
                     /locus_tag="VF874_16820"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9177662.1"
                     /translation="MAEHDFETVSSETVYQGSILALRVDHVKMPGGGIAKREVVEHYG
                     AVAVAAVDDHRRVAMIYQYRHPLGRRLWELPAGLLDAADEDPLDTAKRELQEEAGLAA
                     TDWRVLTDTASSPGFSDESVRTYLATGLSQVDRPDPEHEEADLSLHWFSLEDAAGMVL
                     AGEIVNSTASAGVLAAYAVVIDGQPSRPLYAPWRDRPTAFARRQARG"
     gene            2352..3299
                     /gene="xerD"
                     /locus_tag="VF874_16825"
     CDS             2352..3299
                     /gene="xerD"
                     /locus_tag="VF874_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX9177663.1"
                     /translation="MTTVAAALDGQLRGYLDHLTIERGVAANTLSSYRRDLRRYQEHL
                     TQRGVHDLADVTETDVSEFLVSLRRGDPDAGVAELSAVSAARALIAVRGLHRFAAAEG
                     VTAVDVAKEVKPPTPSRRLPKSLTVDEVLALLTGAGGDSPSDGPLTLRNRALLELLYS
                     TGARISEAVGLDVDDIDTHARSVLLRGKGGKHRLVPVGRPAIDALDAYFVRGRPELAR
                     RGRGTAAIFLNARGGRLSRQSAWQVLQDAAERAGITAGVSPHTLRHSFATHLLDGGAD
                     VRVVQELLGHASVTTTQIYTLVTVHALREVWAGAHPRAH"
     gene            3583..4131
                     /locus_tag="VF874_16830"
     CDS             3583..4131
                     /locus_tag="VF874_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020171695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX9177664.1"
                     /translation="MTSVHLPKLLSLKDWDALPEDNGAHFELQEGVLIVSPSPSRPHA
                     RATFRIAKQLDDQLPDDLEAIIEFEVCVDAAYPPTVRVPDVVITRKDGSERRLDGPDV
                     LVAIEVVSLGSKRTDTVTKRSEHAEAGIDHYWIVALGPPVTLIAFHRAGEFGYHESPT
                     FSGIFTTSAPAPLRLDLDALGE"
     gene            complement(4194..7433)
                     /locus_tag="VF874_16835"
     CDS             complement(4194..7433)
                     /locus_tag="VF874_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9177665.1"
                     /translation="MYPSIDLESVHSRLGVDTYRRGLEYARDGRVVRCLWNPDAGSLV
                     GNVRGNRGCTYATTVQLSPDLADTWTVDIGLCSCPMQVDCKHVAAVVIAVTGITKTGP
                     TRAGQRAAPMRPAPSVWRQSLDALLPNTSASEAFTTPLAIELNLTASGPSPTLDARLV
                     RPGKRGGWVAGDLSWSRVAMLRHFGYPDAHVHLLQEFYAAYRASASNSTGYYSYSYAD
                     MKTISLLKFESPQLWPLLDEARRVGVRLVQPRAQHDVPAYATARLCLDVTADESGDLA
                     VTPVLQVDGATATPVAFIGSSGHGVVYTDRGLRLARLDKPAPKALQRMALGDEPLVIP
                     GHEAARFAAEYYPRLRHIAAFTSSDASFTPPLITGPTLVLRADYRVAHELELTWEWTY
                     RVGDSDFRAPVDTTVDAGCRDLDAENRLASSIDAPLELFGLRAADGKLIARTRLSGLE
                     TMRFATELQPLLAELPDVALEVTGDPVDYREAGGSLVIGVTTDAVPGETDWFDLGVTI
                     TVEGKQVPFVTVFTALASRQSHLLLPDGAYFALDKPELVKLRELIEEARALTDPDEGP
                     TRISRFQAGLFDELAELGVVTRQADEWRRQVDGLRALQGLEPVAVPSSLRADLRPYQA
                     DGFSWLATLHTHGLGGILADDMGLGKTVQSLALICHVRQENSSIAPFLVVAPTSVVAN
                     WATEAARFAPGLSVVTIADTLRRGGADLDELVAGADIVVTSYTLFRLDFEAHADQAWS
                     GLILDEAQYVKNRHAKTYQCARKLAAPIKLAITGTPMENNLMELWSLLSITAPGLFPS
                     PTKFADYFAKPIEKTGDPELLALFRRRIKPLVKRRTKELVAADLPAKQEQILDIDLPP
                     RHRTLYDKRLQRERQKVLGLLDDMQRNRFTILKSLTVLRQMALHPGLVDAAHDTLACA
                     KIDGLAEHLRDIAEGGHRALVFSQFTRFLGRVRDRLGSEGIDYCYLDGRTRDRATVVQ
                     RFKDGYAPVFLISLKAGGFGLNLTEADYCFLLDPWWNPATETQAIDRTHRIGQTRNVM
                     VYRLIARDTIEDKVMALNARKAKLFASVMDDGNAFGSTLTADDIRGLLV"
     gene            complement(7552..>7806)
                     /locus_tag="VF874_16840"
     CDS             complement(7552..>7806)
                     /locus_tag="VF874_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX9177666.1"
                     /translation="RLDFVFVDHDKNAYLPDLLSIMACGWLHPGTIVVADNVGMPGSP
                     KYRAYMRERQGTHWDTREHKTHVEYQTLLGDLVLESEYLG"
BASE COUNT         1315 a         2561 c         2648 g         1282 t
ORIGIN      
        1 agcctcgcgc cacgcgtcac ttgttcgtaa ccggcggggt cgcgtcctcg ctgggcaaag
       61 gcctcaccgc atcgagcctc ggtcagctcc tcacctcccg cggcctgcgg gtgacgatgc
      121 agaagctcga cccctacctc aacgtcgacc ccggcacgat gaaccccttc caacacggag
      181 aggtgttcgt caccgaggac ggcgccgaaa ccgacctcga cgtcggccac tacgagcgct
      241 ttctcgaccg aaatctgtcc gcgcaagcga atgtcacgac cggccaagtg tattcgtcgg
      301 tgatcgcaaa ggagcggcgc ggcgaatacc tgggcgacac cgttcaggtg atcccgcaca
      361 tcaccgacga gatcaagagc cgcatcctcg ccatggcgca acccgacgcc gacggcaacc
      421 gacccgatgt ggtgatcacc gagatcggcg gaaccgtcgg tgacatcgaa tctcagccgt
      481 tcctggaggc ggcgcgacag gtacgccacg atgtcggacg cgacaacgtg ttctttctgc
      541 acgtgtcgct ggtgccgtat ctggcacctt cgggcgaact gaaaaccaag ccgacgcagc
      601 actcggtggc cgcactgcgc agtatcggta tccagccgga tgcgttgatc ctgcgatgcg
      661 accgtgacgt gcccgagccg ctgaagaaca agatcgcgtt gatgtgcgac gtcgacatcg
      721 acggcgtcat ctccactccc gacgcgccat cgatctacga catccccaag gtgttgcacc
      781 gtgaagagct cgacgcgttc gtggtccgtc ggctgcactt gccgttccgc gacgtcgttt
      841 ggaccgagtg ggacgatctg ctgcgacgag ttcacgaacc gggtgagacg gtgcgaatcg
      901 cgttggtggg caagtacgtt gacctatctg acgcatacct gtcagtggcg gaggcgttgc
      961 gggccggcgg attcaagcac tgggccaagg tcgaaatgcg ttgggtcgcc tccgatgact
     1021 gccagaccga cagcggtgcc gtggcggcgc tcgccgatgt gcacggggtg ctgatcccgg
     1081 gcggcttcgg catccggggc atcgagggca agatcggcgc ggtccgccat gcccgcaccc
     1141 acggcctgcc ggtgctgggc ctgtgcctgg gcctgcagtg catcgtgatc gaggccgcgc
     1201 ggtccgtcgg gctgacgcaa gccaattccg ccgagttcga cccgcagact ccggacccgg
     1261 tgatctcgac gatggccgat caggaacaca tcgtcgccgg gcaagccgac ttgggcggca
     1321 cgatgcggct gggcgcctac cccgcggtgc ttgagccgga ttctgttgtg gccgaggcgt
     1381 atcaggccac cgaggtgtct gagcggcacc gacaccgcta tgaggtgaac aacgactacc
     1441 gggaccagat cgccgccagc gggttgcggt tctccggcac gtcgccggac gggcgcctcg
     1501 tcgagttcgt cgagtacccc cgcgaggtca atccgttcct cgtcggcaca caggcacatc
     1561 ccgagttgaa aagccgaccc acccggccgc atcctctgtt cgtctcgttc atcggtgcgg
     1621 cgctggatta caaagcggca gaacggctgc cggtggagat tcccgagcag cggccgaacg
     1681 gcaatgagca cccggctgga gtcgacgggc tgctgcacca gcaagtggcc cgtggctgaa
     1741 cacgacttcg agaccgtctc gtccgaaacg gtctaccagg gaagcatttt ggcgctgcgc
     1801 gtcgaccacg tgaaaatgcc cggcggcggg attgccaaaa gggaggtcgt cgagcactac
     1861 ggcgccgtcg cggtcgccgc cgtggacgat caccgacgcg tggcgatgat ctaccagtat
     1921 cgtcacccgc tcgggcgtcg actgtgggaa cttcccgccg gtctcctgga cgcagccgac
     1981 gaggatccgc tcgacaccgc caagcgcgaa ctgcaggagg aagccgggct cgccgcgacc
     2041 gactggcgag tgctaacaga caccgcgtcg tcgccgggct tctcggacga atcggtgcgc
     2101 acgtacctgg ctaccggact gtcccaggtc gaccggccgg acccggaaca cgaagaagcc
     2161 gacttgagtt tgcactggtt ctcgctggag gacgctgcgg gcatggtatt ggccggcgag
     2221 atcgtgaact ccaccgcgtc agcgggcgtg ctcgccgcgt acgcggtggt gatcgacggc
     2281 cagccgagtc gccctctcta tgcgccatgg cgcgaccggc ccaccgcgtt cgcccgacgt
     2341 caggcgcgcg gatgaccacc gtcgcggccg cactggacgg ccaactgcgg ggttaccttg
     2401 accacctcac catcgagcgc ggcgtggccg cgaacacgct gagctcatac cggcgtgatc
     2461 tgcgccgcta ccaggaacat ctgacgcagc gtggcgtgca cgacttggcc gacgtgaccg
     2521 agaccgacgt cagcgagttc ctggtgtcgc tgcgccgcgg cgatcccgac gctggcgtcg
     2581 ccgaactgtc cgcggtgtcg gccgcgcggg cgctgatcgc ggtgcgcggg ttgcaccggt
     2641 tcgccgcggc cgaaggggtg acggccgtcg acgtcgccaa ggaggtcaag ccgccgacgc
     2701 cgagccggcg gctgcccaag agcctgaccg tcgacgaggt gctggcgctg ctgaccgggg
     2761 ccggcggcga cagcccgtcc gacggcccgc tgacgttgcg caaccgggcg ctgctggaac
     2821 tgctgtactc caccggtgcg cggatctcgg aggcggtcgg gctggacgtc gacgacatcg
     2881 acacccacgc acggtcagtg ctgctgcgcg gcaagggcgg gaagcatcgg ctggtgccgg
     2941 ttggtcggcc cgcgatcgac gcgctggatg cgtacttcgt gcggggccgg ccggagttgg
     3001 cgcgccgcgg ccggggcaca gcggccatct tcctcaacgc ccgcggcggc cggctctccc
     3061 ggcagagcgc atggcaggtc ctgcaagatg ccgcggagcg ggccgggatc actgccgggg
     3121 tgtcgccgca cacgctgcgg cattcgttcg ccacgcacct gctggacggc ggcgccgacg
     3181 tgcgcgtggt gcaggaactg ttgggccacg cgtcggtgac caccactcag atctacacgc
     3241 tggtgaccgt gcatgcgctg cgcgaagtct gggcaggcgc gcacccgcga gcgcactgag
     3301 acgatgcgcg aggcccacca cgtcgtcatc gtcggtgcgg gatcggcgcg ctgtcgaccg
     3361 cgttgagcct gcgcggccgc ggcaggcggt cgcgggccgg aaacagcggc ttgccattcg
     3421 ggtagcggca acccggcagg tgggataacg ggcggccgct gttgagcttg agccggtcgt
     3481 atcagcctcg ccaggtggcg gccacctgct cggccctgtc aacgacgatt ggtcacgcaa
     3541 cactcgactc gaagcgcaac ggtcagagga ggcgggaaga cgatgacgag cgtgcacctg
     3601 ccgaagcttc tgtcgctgaa ggactgggac gctctccccg aggacaacgg tgcccacttc
     3661 gagttgcagg agggcgtgct catcgtgagt ccgagcccat cccggccgca cgcgcgggcc
     3721 acgtttcgca tcgcgaagca gctcgacgat cagctacctg atgatctgga ggcgatcatc
     3781 gagttcgaag tctgtgtcga tgccgcctac ccgcccaccg tccgcgtgcc cgatgtcgtg
     3841 atcacccgca aggacggttc ggagcgccgt ttggacggac cggatgtgct ggtggcgatc
     3901 gaggtggtct cgctgggttc caagcggacg gataccgtaa ccaaacggtc ggaacacgcc
     3961 gaggccggga tcgaccacta ctggatcgtt gcgctcggcc cgcccgtgac actgattgcg
     4021 ttccaccgcg caggcgagtt cggttaccac gaatcaccaa cgttcagcgg catattcaca
     4081 accagtgctc ccgcaccatt gcgtctcgac ctagacgcgc tcggtgagtg atttgcgtcg
     4141 gctggacgaa acagcggtgc agatccggcg tgctgaggtg cccgattggc cgatcagacg
     4201 agcagtccgc ggatgtcgtc ggcggtgagg gtagagccga aggcgttacc gtcgtccatg
     4261 acgctggcga acaacttcgc tttgcgggcg ttgagggcca tgaccttgtc ttcgatggtg
     4321 tcacgagcga tgagccggta caccatgacg ttgcgggtct ggccgatgcg gtgggtgcgg
     4381 tcgatggcct gggtctcggt ggccgggttc caccacggat cgagcaggaa gcagtagtcg
     4441 gcctcggtga ggttcagccc gaacccgccg gccttgaggc tgatcaggaa aaccggcgcg
     4501 tatccgtcct tgaaccgctg gaccaccgtc gcgcggtcgc gggttcgccc atcgaggtag
     4561 cagtagtcga ttccctcgct gccgaggcga tcacggaccc ggccgaggaa gcgggtgaat
     4621 tgactaaaga caagcgcgcg atgaccgcct tcggcgatgt cgcgtaggtg ctcggcgagc
     4681 ccatcgatct tcgcgcaggc cagcgtgtcg tgcgcggcgt cgacgagtcc ggggtgcagc
     4741 gccatctggc gtagcacggt cagggatttc aggatggtga atcggttgcg ctgcatgtcg
     4801 tcgagcaggc cgagcacctt ctggcgttct cgttgcagcc gcttgtcgta gagcgtgcgg
     4861 tgccgaggcg gcaggtcgat gtcgaggatc tgttcctgtt tggcgggcag atcggcggcc
     4921 accagctcct tggtccggcg cttcaccagc ggcttgatac ggcggcggaa cagggcgaga
     4981 agctcggggt cgccggtctt ctcgatgggc ttggcgaagt aatcggcgaa cttcgtcggg
     5041 ctcggaaaca acccgggcgc ggtgatcgat agcagcgacc acagctccat gaggttgttc
     5101 tccatcggtg tgccggtgat cgcgagtttg atcggcgcgg ccagcttgcg tgcgcactgg
     5161 taggtcttgg cgtggcggtt cttcacgtac tgggcttcgt ccaggatcag cccggaccag
     5221 gcctggtcgg cgtgggcctc gaagtcgagc cgaaacagcg tgtatgaggt gacgacgatg
     5281 tcggcgcccg cgaccagctc gtcgaggtcg gcgccgccgc ggcgcagggt gtcggcgatc
     5341 gtgaccaccg acaggccggg cgcgaaccgc gcggcctcag tggcccagtt cgcgactaca
     5401 ctcgtcggcg ccacgaccag gaacggcgcg atgctcgaat tctcttgtcg gacatggcag
     5461 atcagtgcga gcgactggac ggtcttgcca aggcccatgt catcggccag gatcccgccg
     5521 agcccatggg tgtgcagggt cgccagccac gagaaaccat cggcctgata tggccgcaga
     5581 tcagcgcgca ggctgctcgg caccgcgacg ggttccagac cctgcagcgc gcgcaacccg
     5641 tcgacctggc gccgccattc gtctgcctgc cgcgtgacaa ctccgagctc ggcgagttcg
     5701 tcgaacagtc cagcctgaaa ccggctgatc cgggtcggcc cctcatcggg atcggtcagc
     5761 gcgcgggcct cctcgatcag ctcgcgcagc ttcaccagct ccggcttgtc cagcgcgaag
     5821 tatgcgccgt cgggaagcaa gaggtgcgac tgcctggacg cgagagcggt gaacacggtg
     5881 acgaacggca cctgtttgcc ctctacggtg atcgtgacgc ccaggtcgaa ccagtccgtc
     5941 tcgccaggca ccgcatccgt ggtcactccg atgacgaggg agccgcctgc ctcccggtag
     6001 tcgaccgggt cgccggttac ctcaagcgcg acatcgggaa gctcggcgag cagcggctga
     6061 agctcggtgg cgaaccgcat tgtctccagg ccggacaatc gcgtccgggc tatgagcttg
     6121 ccgtcggcgg cgcggagacc gaacaactcc aacggggcgt caatactcga ggcgagcctg
     6181 ttctccgcgt cgaggtcgcg gcagccggca tcaacggttg tatcgacggg tgcgcgaaag
     6241 tcgctgtcgc ccacccgata tgtccattcc cacgtcaact ccagctcgtg ggcgacccgg
     6301 tagtcggcgc gcagcaccaa cgtcggccca gtgatcagcg gcggcgtgaa cgacgcgtcc
     6361 gacgacgtga atgccgcgat gtgccgcaac ctcgggtagt actcggcggc gaaccgcgcc
     6421 gcctcgtgcc cgggtatcac cagcggttca tcgccgagcg ccatacgctg caacgccttc
     6481 ggcgctggct tgtccagtcg cgccagccgc aacccgcggt cggtgtagac gacgccatgg
     6541 ccggacgatc cgatgaacgc gaccggggtt gccgtggcgc catcgacctg aagcacgggc
     6601 gtgacagcga gatcgcccga ctcgtcggcc gtgacgtcga ggcacaaccg tgccgtggca
     6661 tacgccggta catcgtgctg tgcccgcggc tgcaccagcc gcaccccgac tcgccgcgct
     6721 tcgtccagca gcggccacag ctgcggcgac tcgaacttca acagcgagat cgtcttcatg
     6781 tccgcatacg aatagctgta gtagcccgtc gaattcgagg ccgacgcccg gtaggccgcg
     6841 tagaactcct gcagcaggtg aacgtgcgcg tcgggataac cgaagtgccg cagcatggcc
     6901 accctgctcc agctcagatc acccgcaacc cagccgccgc gtttgcctgg gcgcaccagc
     6961 cgcgcgtcca gcgtcggcga cggcccgctc gccgtcagat tcagctcgat cgccagcggt
     7021 gtcgtgaagg cttcgctcgc ggaggtgtta gggagcaggg catccagcga ctgccgccac
     7081 accgatggag ctggcctcat cggcgctgcg cgctgtccgg cgcgcgtcgg accggtcttc
     7141 gtgatcccgg tgacggcgat gacgacggcc gcgacgtgct tgcagtccac ctgcatcggg
     7201 cagctgcaga gcccgatatc gacggtccag gtgtcggcga gatccggaga cagttgcacc
     7261 gtggtcgcgt acgtgcagcc ccggttacca cgaacgttgc cgaccaggct gcccgcgtcc
     7321 gggttccata ggcaacgcac tacccgtccg tcgcgcgcat actcgaggcc acggcgatac
     7381 gtgtcgacgc ccagccgcga gtggacgctc tccaggtcaa tcgacggata catgagcgac
     7441 tgcacgcccg caccgtaacg acccggtccg acagaaggcg ccaaccacgc cgcacgaccc
     7501 ctgaaagtgg tgcagatgag gttcaggagc cttctgttca ggcgtgaggc gttagccgag
     7561 atactccgat tccagcacca gatcgcccag cagagtctgg tactcgacgt gcgtcttgtg
     7621 ttcgcgggtg tcccagtgcg tgccctgccg ctcacgcatg tatgcgcggt acttcggcga
     7681 gccgggcatg ccgacgttgt cggcgacgac gatcgtaccg ggatgcagcc agccgcacgc
     7741 catgatactg agcaggtcgg gcaggtacgc gttcttgtcg tggtcgacga acacgaagtc
     7801 gaggcg
//
LOCUS       DASWBF010000460         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_634184,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000460 DASWBF010000000
VERSION     DASWBF010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634184"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..183)
                     /locus_tag="VF874_16845"
                     /pseudo
     CDS             complement(70..183)
                     /locus_tag="VF874_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026155209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            297..1592
                     /locus_tag="VF874_16850"
     CDS             297..1592
                     /locus_tag="VF874_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX9177667.1"
                     /translation="MKTVPTVRLADMTDAATLPDLPEEIRLAMTDIAGAARDGLMAMS
                     VAAGLAVMQVMFEAEIAGVAGPKGKHDPGRAALRHGQGRGSVTLGGRRVAVDRPRART
                     VDGHEVPLTSYTQFASDDLLSQVVMERMLAGVATRRHARTGEPVGTQVVEQAKSTSRS
                     AISRRFVRQTETALGELMARDLSGEDIKVLMLDGEHMAERCVVVALAITADGTKKPVG
                     LWDGSTENKTVVRALLADLVARGLAFDDGLLVVLDGAKALSAAVREVFGDKALIQRCT
                     LHKRRNVADHLPDKDKAWVDAKLVKAFGHADPDTGLRNAKSLAAQLEKNYPGAAASLR
                     EGLDEMFTVARLGIDGRLAKTLTTSNPVESMISIARATNRNVTRWRDGQMVLRWTAAG
                     MLNAERSFRRIKGHKQMPQLVAALRRHAHPDTESVGTAA"
BASE COUNT          327 a          614 c          667 g          298 t
ORIGIN      
        1 ccgctacgct gcccgaagcc gcctaaccac aaatcccgtg tccactacat ggggtcaagg
       61 cccgacccgg atgtcgagca cgttgccgtg ctggtcgacc gcacgccaca ggtagtggat
      121 cgtgccgttg atccggatga agacttcatc gagatgccat ttgtcgccgg ggcgggggcg
      181 ccgcgaggtt gtcccggtct tcgtggaatt tgaggtggcg gtccctcacc tgaggcccgc
      241 cagatggtag gtcttcgggt gaaccgccaa gtacacccac gaaggaaggg accgccatga
      301 aaaccgtacc cactgttcgt ctcgctgaca tgaccgacgc cgcgacgctg cccgatctgc
      361 cggaggagat ccggctggcg atgaccgata ttgccggtgc cgcgcgtgat gggctgatgg
      421 cgatgagcgt tgccgccggg ctggcggtga tgcaagtaat gttcgaggcc gagatcgctg
      481 gggtggctgg cccgaagggc aagcacgacc cgggccgggc cgcgctgcgc catgggcaag
      541 gccgcggctc ggtcaccctg ggtgggcgca gggtggcggt ggaccggccg cgggcgcgca
      601 ccgtcgacgg gcatgaggtg ccgttgacca gctacacgca attcgcctcc gatgacctgc
      661 tgtcgcaggt ggtgatggag cggatgctgg ccggggtggc cacccgccgg cacgcccgca
      721 cgggtgagcc ggtcggcacg caggtggtcg agcaagcgaa atcgactagc cgctcggcga
      781 tttcgcgtcg gttcgtgcgt cagaccgaga ccgcgctggg cgagctgatg gcccgcgacc
      841 tgtccgggga ggacatcaag gtgctcatgc tcgatggcga gcacatggcc gagcggtgtg
      901 tcgtggtcgc gctggccatt accgctgacg gcaccaagaa gccggtcggg ctgtgggacg
      961 gctccacgga gaacaagacc gtggtgcgcg ccctgctggc cgacctggtc gcgcgcggcc
     1021 tggcttttga cgacgggctg ctggtggttc tcgacggcgc caaggcgctg tcggcggcgg
     1081 tgcgcgaggt gttcggcgac aaggccctca tccagcgctg tactttgcac aagcgccgca
     1141 acgtcgccga tcacctgccc gacaaggaca aggcgtgggt ggatgccaag ctggtcaagg
     1201 ccttcggcca cgccgacccc gacaccgggc tgcgcaacgc gaaaagcctt gcagcacaac
     1261 tggagaagaa ctatcccggt gcggccgcgt ccctacgcga agggctcgac gagatgttca
     1321 ccgtcgcccg cctcggcatc gacggccgcc tggccaaaac gttgaccacc agtaacccgg
     1381 tcgagtcgat gatctccatc gcccgcgcca ccaaccgcaa cgtcacccgc tggcgcgacg
     1441 ggcagatggt gctgcgctgg accgccgccg gcatgctcaa cgccgagcga tccttccgcc
     1501 gcatcaaggg ccacaagcag atgccccaac tcgtcgccgc cctgcgccga cacgcccacc
     1561 ccgacaccga atctgtcggt accgccgcct agagttcacc gtggatcgtc acccaaggtc
     1621 cacgcgactc gggagtatgc cctgcaattc gagaggggtt gcgtgatgac gtgatctgac
     1681 tcggttggaa gctcgtcgtt gccagccggt gcgagtccgg tccgggtagc cgccaggggg
     1741 cccggtagca gactgccggc gtcgggggga aacgtccggc gtcgaagtgc agtgtcgaaa
     1801 gcccttgtga gggggagcaa ctgagcggtc cgcagcatta agcgaagcct gccgcgtcgt
     1861 gaatgtgccc gtgtggagcg ggatcaaagg agagccgagc cccgtc
//
LOCUS       DASWBF010000461        13975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1232203,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000461 DASWBF010000000
VERSION     DASWBF010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13975
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232203"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VF874_16855"
     CDS             complement(<1..594)
                     /locus_tag="VF874_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9177668.1"
                     /translation="MTPLTNPISVVVIGGGYAGTVAANRLQMRKDVGVTLVNPRPWFV
                     ERIRLHQLVADSGSATVDYADMLNPKVHLAIDAATEINATSRTVRLASGGGLRYDYLI
                     YAVGSTGAVPSVPGAPQFAWQISDFEHAHSLRGRVAELHPEAAVCVVGGGLTGIEASA
                     ELAEQRPEGTVTLICGPTLGPSLSEPGRRSVARQLARL"
     gene            complement(682..1254)
                     /locus_tag="VF874_16860"
     CDS             complement(682..1254)
                     /locus_tag="VF874_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9177669.1"
                     /translation="MTIEERRARDRAARRQLIVTTARKLAETEGWDAVTTRRLSTEIE
                     YSQPVIYKHFSGMDAVASTVAIDGFGELANTLSKARAAGEPEHALRRIAHAYIDFARH
                     NPALYEAMFTRATTLHFAADDTPRELLAAFGELRAAVEAISDQSDLDTATEVVWAALH
                     GLATLSRSGRLRPQQEFERIELLADQLTKQ"
     gene            1354..1776
                     /locus_tag="VF874_16865"
     CDS             1354..1776
                     /locus_tag="VF874_16865"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9177670.1"
                     /translation="MTANPHRLVEAFVTMVNEHDPDLVDRFVAVDYINHNVFVPDGRE
                     ANRRFWASFFTALPDVTATVEDLVISGDRVVGRFIYRGTHAGEFIGIPATGQRVEMRS
                     IDIWRVEGGMFNEHWDELNTLQLLQQMGALPNQMGGAA"
     gene            1773..2243
                     /locus_tag="VF874_16870"
     CDS             1773..2243
                     /locus_tag="VF874_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEX9177671.1"
                     /translation="MIDLARTAALVAVLAAAVVYGTDVFCAVVQRPALAHVDDRALVA
                     VMGNLHLYGDRRMPVPGALSIIAAAVSGALAALTGRWVAAGAAVSAVGLLLIWIAMYI
                     RVSAPINRQLTTAAKAGDVPANARVLQSDWDRVITARAILQGLAIVALCVALVN"
     gene            2246..2563
                     /locus_tag="VF874_16875"
     CDS             2246..2563
                     /locus_tag="VF874_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX9177672.1"
                     /translation="MFQLRIYTLRTDEALERYATIHWARHVGSLAAFGVTTHGIWTER
                     DGSANRLVALVSFAQDADPAAVTNEYMASPEFNADMEGFDRRDVVAVTTVLLDATGSS
                     PIR"
     gene            complement(2579..4156)
                     /gene="metG"
                     /locus_tag="VF874_16880"
     CDS             complement(2579..4156)
                     /gene="metG"
                     /locus_tag="VF874_16880"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9177673.1"
                     /translation="MRPFYVTTAIDYPNGAPHIGHAYEKIATDAIARFKRLDGFDVRF
                     LTGTDEHGQKMEEAAVAEGMPTAQLAGRNSDVFQRLYDRLNLSYDRFIRTTDPDHLEA
                     SKVIWQRMWDAGDIYLGTYSGWYSIRDENFVSEDETRVEADGTRVAVDSGAPVTWTEE
                     ETYFFRLSAYTDKLLAHYEAHPEFIGPDVRRNEVVSFVSGGLRDFSISRTTFDWGVPV
                     PDHPNHVMYVWVDALTNYLTGVGFPDIDSEMFRRFWPADLHMIGKDIIRFHTVYWPAF
                     LMSAGIELPRRVFAHGHLLNRGVKMSKSVGNVVEPNALVDQYGVDQVRYFLLREVPFG
                     QDGSYSDDAIVTRINADLANEFGNLAQRSLSMVAKNLDGVVPEPTALNDDDRELLAAA
                     DALLERMRAHFDIPAMHLALEAIWLVLGAANKYFSAQQPWVLRKSEAESDRARFDTVL
                     YTTLEVVRIAALLVQPVMPSSAGQLLDLLGQPDEARSFAAIGTRLAPGTALPAPTGVF
                     PRYQATPDDRSDKPRSV"
     gene            4183..5022
                     /locus_tag="VF874_16885"
     CDS             4183..5022
                     /locus_tag="VF874_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9177674.1"
                     /translation="MSRSRPAPPPPEPLFPLVDAHTHLDACGASDADGVRAIVDRAEA
                     VGVNAVVTIADDLASARWVVTAAHADPRVYAAVALHPTRADALTDAAKADIESLARDP
                     RVVAVGETGLDLYWPGRLETCAEPETQRDAFAWHIDLAKRTGKPLMIHNRDADREVLD
                     VLRAEGAPRDVIFHCFSSDSQMARECLDGGWLLSLSGTVSFKNATALHEAARLIPPQQ
                     LLVETDAPFLTPHPYRGAPNEPYCLPYTVRALADLTGRPAEDLARDTTANAVRVYDLT
                     APA"
     gene            5279..6406
                     /locus_tag="VF874_16890"
     CDS             5279..6406
                     /locus_tag="VF874_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX9177675.1"
                     /translation="MNTLTKLHQTNSPVLRALVGALLLVLVVAGSYAVSVHKTVTLDV
                     DGSPMTVSTMKSRVIDVVQENGFTVDERDDLYPAGDEAIHQADTIVLRRSRLLQVSMD
                     GHETRQVWTTASTVDEALQQLSMSEAAPAAASRSSRLPLAGMALPVVSPKNVHIDDAG
                     VVADRRLAAPTVAGLLAAAGAPLEQADKVVPPPSTPVTEGMTVVVTRIRMQKITERVP
                     LPPPAQKIPDPEMNMSRQVVDDPGTPGEQDVTFAVSIVNGVETGRLPVANEVIAPARP
                     SVLRVGAKPGTEVPPVANGATWDALASCEAGGNWAINTGNGFFGGVQFDQNTWERSGG
                     LRYAARADLATREEQITIATVTQQRQGWGAWPVCSGRIGAR"
     gene            6370..7293
                     /gene="rsmA"
                     /locus_tag="VF874_16895"
     CDS             6370..7293
                     /gene="rsmA"
                     /locus_tag="VF874_16895"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9177676.1"
                     /translation="MAGVQRTHRGALTIRLLGRTEIRRLAKDLDFRPRKSLGQNFVHD
                     GNTVRRIATASGVGRTDHVLEVGPGLGSLTLGLLDKGTAITAVEVDPVLAKRLPQTVA
                     EHSNSEAQRLTVLNKDILTLRRADLETAPCGPPTALVANLPYNIGVPALLHLLAEFPS
                     IRVVMVMVQAEVAERLAAEPGGKEYGTPSVKARFFGRVRRCGMVSPTVFWPIPRVYSG
                     LVRIDRYQTPPWPTDEAFRRAVFELVDIAFAQRRKTVRNAFAEWAGSGNESARRLLAA
                     SIDPARRGETLGIADFVRLLQRSEVPAAPGD"
     gene            complement(7290..8078)
                     /locus_tag="VF874_16900"
     CDS             complement(7290..8078)
                     /locus_tag="VF874_16900"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9177677.1"
                     /translation="MLCPGIEGYVVEDVLGHGGSATVYRARDEARRVVALKVLDDRYR
                     GPAETARLQREFEFAQRLSHPHVVSVYRHGEDWLTMQLVDGGTAMGLKELDDRLTAME
                     QIADALDYVHRCGIVHCDVKPSNVLVHKYFSHGGAVLTDFGVAYAVAEDNRSRPQPLM
                     ASLPYLAPEMLRGRPPAAATDEYALACTTVELVTGAPPFVAATPADLAEAHLNDPPPY
                     PSRRYEWLPRAFDGVIGKAMAKDPELRYPSCAEFVVAVSRVLKR"
     gene            8232..9185
                     /locus_tag="VF874_16905"
     CDS             8232..9185
                     /locus_tag="VF874_16905"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX9177678.1"
                     /translation="MSDGSTAPEWVATGSVTVRVPGKVNLYLAVGDRRDDGYHELTTV
                     FHAVSLLDEVTVRNSDTLSVEVTGEGADTLPSDERNLAWLAAELMAEHVGRSPDVEIA
                     IEKSIPVAGGMAGGSADAAAVLVAMNSLWEVGVPRRDLHALAARLGSDVPFALHGGTA
                     LGTGRGEDLATVLARTTFHWVLAFSASGLSTAAVFAELDRLRKGGDPPRLAEPEPLLA
                     ALAAGDANELAPLLGNDLQAAAISLDPSLRRTLRAGAEAGALAGVVSGSGPTCAFLCP
                     TASSAIDVGTQLAGAGVCRTVRVASGPVQGARVVPAPTHGV"
     gene            9473..11107
                     /locus_tag="VF874_16910"
     CDS             9473..11107
                     /locus_tag="VF874_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX9177679.1"
                     /translation="MSRFTEKMYRNALRTDRGMVTGEPHEPVRHTWGEVHEKARRIAG
                     GLAAAGVGHGDAVGVLAGAPVEIAPTAQGLWMRGASLTMLHQPTPRTDLALWAQDTTT
                     VIDMIEAKAVVISDPFTVAIPVLEERGVKVVTVEQLLAADPIDPVETGEDDVALMQLT
                     SGSTGSPKAVVITHRNVYSNAEAMFIGAEYDVDRDVMVSWLPCFHDMGMIGFLTVPMY
                     FGAELVKVTPMDFLRDTLLWAKLIDKYKGTMTAAPNFAYALFAKRLRKQAKPGQFDLS
                     TLRFALSGSEPVEPADVEDLIDAGRPFGLPAEAILPAYGMAETTLAVSFSECGAGLVV
                     DEVDTDLLAALRRAVPATKGNTRRLASLGPLLKDLEARIVDEDGNVLPTRGVGIIELR
                     GEALTPGYITMGGFVPAQDNHGWYDTGDLGYLLENGHVVVCGRVKDVIIMAGRNIYPT
                     DIERAASRVDGVRPGCAVAVRLDAGHSRETFAVAVESNAWEDPADVRRIEHQVAHEVV
                     GEVDVRPRNVVVLGPGTIPKTPSGKLRRANSVALVS"
     gene            11296..12018
                     /locus_tag="VF874_16915"
     CDS             11296..12018
                     /locus_tag="VF874_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX9177680.1"
                     /translation="MSNTFFTRIAAAAFIGLATVGATGATVSFAGPAMAAPAPSVGPS
                     TTGMYGDPTEAAEFWAEQSLDDCTLMAIASVVGQVTGDMPTEAEIIELAENTPSTTHP
                     GSIYIRPANPEDPNTGNGTDPHDAVVLLAHYGIDAEVTDADSEDEAGVDTGMRALEQY
                     LAAGHKVIVSVNAETIWDIDGPRNQSNHSVSVIGVDVKNGIVHLNDSGTEDGRDEQVD
                     IATFMKAWKTSDYRMIVTEEAA"
     gene            12203..12916
                     /locus_tag="VF874_16920"
     CDS             12203..12916
                     /locus_tag="VF874_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX9177681.1"
                     /translation="MNTTFSTRIAHAARTAVVAVVIGASGAAGIACASVANASPVIEV
                     TTPDGVYGAPDAAAEFWQRQHLSDDCVLMAVADVVGQITGDLPSEQEILEVAADTKSN
                     SHSGPVYLPDDNRWGTDYGDAEVLLAEYGITGTMTSTAIDEQTGVETGMDALIEYLAT
                     GEKVIVGLNGPTVWDTSGDRKKPAHAVVVTGVDTVHDVVHLNDPYPADGRDLEVPLAT
                     FEKAWRTGPFIMIVTDEAA"
     gene            complement(12897..>13975)
                     /locus_tag="VF874_16925"
     CDS             complement(12897..>13975)
                     /locus_tag="VF874_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9177682.1"
                     /translation="GPPTQALEYFGCVDFPDLFTLLERDTTTNWAGRLANSPFYGTYI
                     RPATPPVGRQAVASAAPAKPLPAIAQQSPFDQFAILCRRYLSVIAGDRQYSVFMLALP
                     LLLSIFLYAVPGSAGLSVLRGIAQHSQQPRQLLVLLIIGGALMGCAASIREIVKEQAI
                     YRREHTIGLSRGAYLASKLVILTMLTGLQGLALGVLGVVFLPGPDEPLLIPSARLEVA
                     FAVATISVVSMVVGLLISAMIDNADRGMPLLVLVVMAQLILCGGLFPVRDQVPMGQLS
                     WLSPSRWAYAMGAATVNLNTISSPGTDDPLWRHTLGAWLTAFGMCLVLAVVMVALLVA
                     ALSRLDPHRKSRSGKPMAVTRLRR"
BASE COUNT         2352 a         4653 c         4724 g         2246 t
ORIGIN      
        1 gaggcgggcg agctgacggg ccaccgatcg ccgaccgggt tcgctcagcg acgggcccag
       61 cgtcgggccg cagatcagtg tcactgtgcc ttctggccgc tgctcggcga gctcggcgga
      121 cgcctcgatt ccggtcagcc cgccgccgac cacgcacacc gcagcttcag ggtgtagctc
      181 ggcgacgcga ccccgcaaac tgtgagcatg ttcgaaatcg cttatttgcc aggcgaattg
      241 gggagcaccc ggcacgcttg gcacagctcc cgtgctgccg acggcgtaga tgaggtagtc
      301 gtagcgcagg ccgccgcctg atgcgagccg aacggtgcgg ctggtcgcat tgatctcggt
      361 cgcggcgtcg atggccagat gcaccttcgg gttaagcatg tcggcgtagt cgacggtcgc
      421 cgaacctgag tcggccacca actggtgcag ccggatgcgt tcgacgaacc acggccgcgg
      481 gttgacgagg gtgacgccaa cgtccttgcg catctgcaga cggttcgccg cgacggttcc
      541 ggcgtagccg ccgccgatca ccacgacgga gatggggttg gtgagtgggg tcatgtcggc
      601 tcctgtgctc atgtcggtca tgccctgtag acaccggcga tggtcccgac gtgacgccac
      661 gcgacgtgaa tgtggcctac ctcactgttt ggtgagctgg tcggccagga gttctatgcg
      721 ctcgaattct tgctgcggtc gcagtcgacc gctgcggctc aatgtggcca gcccgtgcag
      781 tgctgcccac acgacttcgg tggccgtgtc taaatcggat tggtccgaga ttgcttcaac
      841 cgccgcacgt agctcgccaa aggccgccaa cagctcacgc ggtgtgtcgt cagcggcgaa
      901 gtgcagcgtg gtggcgcggg tgaacatcgc ttcgtaaaga gccgggttat ggcgggcaaa
      961 atcaatgtac gcgtgcgcaa ttcgtcgcag cgcgtgttcg ggctctccgg cagcgcgggc
     1021 tttggaaagg gtgttggcca actcgccgaa tccgtcgatc gcgaccgtcg aggcaaccgc
     1081 gtccatgccc gaaaaatgtt tgtagatcac gggctggctg tactcgatct cggtggacag
     1141 ccgacgcgtc gtcaccgcgt cccacccctc cgtctccgcc agctttcgtg ccgtcgtcac
     1201 gatgagctgg cgccgcgcgg ctcggtcgcg ggcacgacgc tcctcaatgg tcatgcccga
     1261 tggtagcact gctagaaatg aaagcatcgc tattgacacg acgtgaacgc ggggttagcg
     1321 ttgctaacac ctacgcctga ttggagtctt ccgatgaccg ccaacccaca tcgcctcgtc
     1381 gaggccttcg taacgatggt caacgaacac gacccagacc tcgtagacag gttcgtggcc
     1441 gtcgattaca tcaaccacaa cgtgttcgtc cccgacggac gcgaggcgaa tcgcaggttc
     1501 tgggcgagct tcttcacggc cctgcctgac gtgacagcga cagtcgagga cctggtgatt
     1561 tcgggggacc gcgtggtggg ccgcttcatc tatcgcggaa cccacgccgg tgaattcatc
     1621 ggaatccctg cgacgggtca gcgcgtcgaa atgcgatcca tcgacatctg gcgcgtggaa
     1681 ggcggcatgt tcaacgaaca ttgggacgag ctcaacacgt tgcagttgct tcagcagatg
     1741 ggtgcgctgc ccaaccagat ggggggtgca gcatgatcga cctggcacga acggctgcgt
     1801 tggtggcggt gctggccgcg gcggttgtct acggcacgga cgtgttttgc gccgtcgtgc
     1861 agcgtccggc cctggctcac gtcgatgatc gggcccttgt cgcggtgatg ggcaacctgc
     1921 atctttacgg cgaccggcgg atgccggtgc cgggcgcgct gagcatcatc gccgcagccg
     1981 tgagcggcgc gctcgccgca cttaccggtc gatgggtcgc ggcaggcgcg gcggtcagcg
     2041 ccgttggctt gctactgatt tggattgcga tgtacatccg ggtgagcgcg ccgatcaacc
     2101 ggcagctgac caccgccgcg aaagcaggtg acgtcccagc gaatgcgcgt gtgctgcaaa
     2161 gtgactggga ccgggtcatc accgctaggg ccatcttgca gggcctcgcg atcgtggccc
     2221 tgtgcgttgc gctggtgaac tgatcatgtt ccagctgagg atttacacgc tacgtactga
     2281 cgaggcgctc gagcggtacg cgactattca ctgggcccgt catgtcggga gccttgcggc
     2341 attcggagtg acaacccacg ggatctggac cgaacgcgac gggagtgcga atcgacttgt
     2401 cgcgcttgtg tctttcgcgc aagacgccga cccggcagcg gtcaccaacg aatacatggc
     2461 cagtcccgag ttcaatgccg acatggaagg cttcgaccgt cgagacgtcg tggccgtcac
     2521 caccgtgctg cttgacgcga cgggttcttc accgatccgc tgaggcgacc gggctatctc
     2581 acacggagcg aggcttgtcc gagcgatcat cgggcgttgc ctgataccgc gggaacacgc
     2641 ccgtcggtgc cggcagcgcg gttcccggcg ccagtctcgt cccgatcgcg gcgaacgagc
     2701 gcgcctcgtc cggctggccg agcagatcca gcagttgacc ggccgaggag ggcatcaccg
     2761 gctgcaccag cagcgcagcg atgcggacca cctccagcgt cgtgtacagc acggtgtcga
     2821 accgtgcgcg gtcggattcg gcctccgact tgcgcagcac ccacggctgc tgcgccgaga
     2881 agtacttgtt cgcggcgccc agcaccagcc agatcgcctc cagcgccaaa tgcattgcgg
     2941 ggatgtcgaa gtgcgcgcgc atccgctcca agagcgcgtc ggcggcggcc agtagctcgc
     3001 ggtcgtcgtc gttcagcgcc gtgggttcgg gtacgacgcc gtcgaggttc ttggccacca
     3061 tcgacagcga gcgctgcgcg aggttgccga actcgttggc aaggtcggcg ttgatccggg
     3121 tgacgatcgc gtcgtcgctg taactgccgt cctgcccgaa cggaacttcg cgcagcagga
     3181 agtaacgcac ctggtcgacg ccgtactggt cgaccagcgc attgggttcg acgacgttgc
     3241 ctaccgactt actcatcttg acgccacggt tgagcagatg gccgtgcgcg aaaacccttc
     3301 gcggcaactc gattccggcc gacatcagaa acgccggcca gtagacagtg tgaaaccgga
     3361 tgatgtcttt gccgatcatg tgcaggtcgg cgggccagaa ccggcggaac atctccgagt
     3421 cgatgtccgg aaagcccacg ccggtaaggt aattcgtcag cgcgtccacc cagacgtaca
     3481 tgacgtggtt agggtggtcg ggcaccggca cgccccagtc gaacgtcgtg cgggagatcg
     3541 agaagtcgcg caagccaccg gagacgaaac taacgacctc gttgcggcgc acgtccggtc
     3601 cgatgaactc cggatgcgcc tcgtagtggg cgagcagttt gtcggtgtac gccgacaagc
     3661 ggaagaaata ggtttcctcc tctgtccacg tgaccggcgc gccgctgtcc acggctaccc
     3721 gcgtcccgtc ggcttcgacc ctggtttcgt cttcgctgac aaagttttcg tcgcgaatcg
     3781 aataccagcc cgagtacgtg cccagataga tgtcgccggc gtcccacatg cgctgccaga
     3841 tgaccttgga cgcctcaagg tggtccgggt cggtcgtgcg gatgaaccgg tcgtaggaga
     3901 gattgagcct gtcgtacagc cgctggaaca catcggagtt gcggcccgcc agctgcgcgg
     3961 tgggcatgcc ctcggcgacc gccgcttcct ccatcttctg gccgtgctcg tcggtgccag
     4021 tcagaaagcg aacatcgaag ccgtcgagcc gcttgaaccg ggcgatcgcg tcggtcgcga
     4081 tcttctcgta ggcatgcccg atgtgcggcg ccccgtttgg gtagtcgatc gccgtcgtca
     4141 catagaaagg cctcatttcg acgtcaacct tatggtgtgg cagtgagtcg ttcccgcccg
     4201 gcgccgccgc caccggagcc gcttttcccg cttgtcgacg cgcacacgca tctggacgcg
     4261 tgcggcgcga gcgacgcgga cggcgtgcgt gcgatcgtgg accgcgccga ggcggtgggc
     4321 gtcaacgccg tcgtcacgat cgccgacgac ctggcgtccg cgcggtgggt ggtgacggcg
     4381 gcccacgccg acccgcgggt gtacgccgcg gtggcgctgc acccgacgcg cgccgacgcc
     4441 ttgacggacg ccgcgaaggc agacatcgag tcactggcgc gcgatccgcg ggtggtggcc
     4501 gtcggggaga ccggcctcga cctgtactgg ccgggccggc tcgaaacctg cgcggagccg
     4561 gagacgcagc gcgacgcatt cgcctggcac atcgacctgg ccaagcgcac cggcaagccg
     4621 ctgatgatcc acaatcgcga cgccgaccgc gaggtgctcg acgtgctgcg cgccgagggt
     4681 gcgccgcgcg acgtgatctt ccactgcttc tcctccgact cgcagatggc gcgcgaatgc
     4741 ctcgacggcg gctggctgct gagcctgtcg ggcaccgtta gcttcaagaa cgcgaccgct
     4801 ctgcacgagg ccgcccggct gataccgcca cagcagctgc tggtcgagac cgacgcgcca
     4861 tttctgaccc cgcatccgta tcgcggcgcc ccgaacgagc cgtactgcct gccctacacc
     4921 gtgcgggcgc tcgccgacct gacgggccga ccggctgagg acctcgccag ggacacgaca
     4981 gcaaacgccg tgcgggtgta tgacctgacg gcgcccgctt gaccggctcg gcgtgcgtgg
     5041 ccgctccgac ctcgccttta ccgcttaaac cgctgcagca aacacgcaac aaagccgctg
     5101 gtggcgcgtc cggcccggaa actgaccgtc tatgactaaa ggcagtgact gcactgtgag
     5161 ccacgcctca acccgctctt gagatttgcc tctggtaggg gtgttcgtta ccgtcttgtg
     5221 atcagatttg gccgctggcc cctagcaaac gacgtcgata gtcgggatag accagacttt
     5281 gaataccctg acgaagcttc accaaacaaa ttcacctgtt ctgcgcgccc ttgtgggcgc
     5341 gctgttgctg gttctggtgg ttgccggaag ctacgccgtg tccgtgcaca agaccgtcac
     5401 gcttgacgtc gacggctcgc cgatgaccgt atccacgatg aagtcacggg tgatcgacgt
     5461 cgtgcaggag aacggcttca cagtcgacga gcgcgacgat ctttacccgg ccggcgacga
     5521 ggcgatccat caggccgaca cgatcgtgct gcgccgcagc cgcctgctgc aggtgtcgat
     5581 ggacggtcac gagacccggc aggtgtggac caccgcgtcg actgtcgacg aggccctcca
     5641 gcagctgtcc atgtccgagg cggcacctgc cgcggcgtca cgatcgagcc ggctgccgct
     5701 ggccggcatg gcgctgccgg tggtcagccc gaagaacgtc cacatcgacg acgccggcgt
     5761 ggtcgccgac cggcggctgg cagcgcccac cgtcgcgggg ttgctcgccg cggccggagc
     5821 gccgctggag caggcggaca aggtggtgcc gccgccgtcg accccggtga cagaggggat
     5881 gactgtggtc gtcacgcgca ttcggatgca gaagatcacc gagcgggtgc cgctgccgcc
     5941 gccggcgcag aagatcccgg atcccgagat gaacatgagc cgccaggtcg tcgacgaccc
     6001 cggcacgccg ggcgagcagg atgtcacgtt cgcggtgtcc atcgtcaacg gcgtggagac
     6061 cgggcggctt ccggtggcca acgaggtcat cgcgccggca cggccgtcgg tgctgcgtgt
     6121 cggcgccaaa cccggcaccg aggtgccgcc ggtggccaac ggcgcgacct gggacgccct
     6181 ggcctcgtgt gaagcgggcg gcaactgggc gatcaacacc ggcaacgggt tcttcggcgg
     6241 tgtccagttc gaccagaaca cctgggaacg cagcggcggc ttgcggtacg cggcgcgtgc
     6301 ggacctggcg acccgcgaag agcagatcac gatcgcaacg gtgacgcaac agcgtcaggg
     6361 ctggggtgcg tggccggtgt gcagcggacg catcggggcg cgctgacgat ccggctgctg
     6421 ggccgcacgg aaatccgccg gctggccaag gatctcgact ttcgcccgcg aaagtctctg
     6481 gggcagaatt tcgttcacga cggcaacacg gttcgccgga tcgcgacggc gtcgggagtg
     6541 ggccgcaccg accatgtgct ggaagtgggt cccggtctgg gttcgctgac gctgggcctg
     6601 ctggacaagg gcaccgcgat aaccgcagtg gaagtcgatc cggtgctggc caagcgactg
     6661 ccgcagacag tggccgaaca ctccaacagc gaagcccaac ggctgacggt gttgaacaag
     6721 gacatcctca cgcttcgccg cgccgacctc gagactgcgc cctgcgggcc gccgaccgcg
     6781 ctcgtggcca atctgcccta caacatcggc gtcccggcgc tgctgcacct gctcgccgag
     6841 ttcccgtcga tccgcgtggt gatggtgatg gtgcaggccg aggtggccga gcggctggcg
     6901 gctgagcccg gcggcaagga gtacggcacg ccgagtgtga aggcgcgatt cttcgggcgg
     6961 gtgcgtcgtt gcgggatggt gtcgccgacg gtgttctggc cgatcccgcg ggtgtactcc
     7021 ggcctcgttc gcatcgaccg ctaccaaacc ccgccgtggc ccaccgacga ggcgttccgc
     7081 cgagccgtct tcgagctggt ggatatcgct ttcgcgcagc gccgcaagac cgtccgcaat
     7141 gccttcgcag aatgggccgg gtcgggcaac gagtcggccc ggcgactgct ggcggccagc
     7201 atcgacccgg cgcggcgcgg cgagacgctt ggcatcgcgg atttcgtgcg gctgctgcag
     7261 cgatccgaag ttccggccgc gccaggcgac tagcgtttca agacgcggct gaccgcgacg
     7321 acgaactcgg cgcacgacgg gtagcgcaat tccgggtcct tggccatcgc cttgccgatc
     7381 actccgtcga acgcccgggg cagccactcg tacctgcggg acgggtaagg cggcgggtcg
     7441 ttgaggtggg cctccgccag gtcggcgggg gtggcggcga cgaatggcgg tgcgcccgtg
     7501 accaattcga cggtcgtgca cgccagagca tattcgtcgg tggccgccgc cgggggccgg
     7561 ccacgcagca tttccggtgc caggtagggc agcgacgcca tcagtggctg cgggcgcgaa
     7621 cggttgtcct cggcgacagc gtaggcgacg ccgaagtcgg tgagcaccgc gccgccatgc
     7681 gaaaagtact tgtgcacaag cacattggag ggcttgacat cgcagtgcac gatgccacac
     7741 cggtgcacgt agtcgagcgc gtcggcgatc tgctccatcg cggtcaaccg atcgtcgagc
     7801 tctttcaggc ccatggcggt gccgccgtcc accaattgca tggtgagcca atcctcgccg
     7861 tggcggtaca ccgaaactac gtgtgggtgg ctgagccgct gcgcgaactc gaactcccgc
     7921 tgcagccgcg cggtctcggc tggcccgcga taacggtcgt ccagcacctt cagcgcgaca
     7981 acccggcgcg cctcgtcgcg cgcccgatac accgtcgccg agccgccgtg gccgagcacg
     8041 tcttccacga cgtagccctc gatcccaggg cacagcacag cttcagggta ggccgcatcg
     8101 gtttggcggt ggcgccaagt cggttaccca ttgagtctga cgactggtcg agacggccga
     8161 ggggtccgtc tcgccactct gttgcgttgt cgccgggttt cttttggcgc ggggggcgtt
     8221 agtctcgtgc ggtgtccgac ggcagcaccg cacccgagtg ggtggccacc ggttcagtca
     8281 cggtgcgggt gccgggcaag gtcaacctgt acctggccgt cggtgaccga cgcgacgacg
     8341 gctaccacga actgaccacc gtcttccacg cggtgtcact gctcgacgag gtgacggttc
     8401 gcaactccga cacgctgtcg gtggaagtga ccggcgaggg cgccgacacg ttgccctccg
     8461 acgaacgcaa ccttgcttgg ctggccgccg agctgatggc cgagcacgtc ggccgttcac
     8521 cggacgtgga gatcgcgatc gagaagtcca ttcctgtcgc cggtggcatg gccgggggca
     8581 gcgccgacgc ggccgcggtg ctggtggcga tgaacagcct gtgggaggtc ggtgtgcccc
     8641 gccgcgatct gcacgcgctg gccgccaggc tgggcagcga cgtgccgttc gcgttgcacg
     8701 gcggcaccgc gctgggcacc ggccgcggcg aggacctcgc gacggtgttg gcgcgcacga
     8761 cgtttcactg ggtgctcgcg ttctcggcca gcgggctgtc cacggcggcg gtgttcgccg
     8821 agctcgacag gcttcgcaag ggcggcgacc caccgcggct ggctgagccc gagccgctgc
     8881 tggccgcgct ggccgccggc gacgccaacg agctcgcgcc gcttctgggc aacgacttgc
     8941 aggccgccgc gatcagcctc gaccccagcc tgcgccgcac cttgcgggcc ggcgccgagg
     9001 cgggcgcgct ggcgggcgtc gtctcgggat ccggccctac ctgcgccttc ttgtgtccaa
     9061 ccgcgtcgtc ggcgatcgac gtcggcacgc agctggccgg ggccggtgtc tgccgcaccg
     9121 tgcgggtggc cagtggtccg gtgcagggtg cccgcgtggt gccggcaccc acccacgggg
     9181 tgtgacacag gcctcaaaac cgctctcggt ttgccgctta ctctaagagg agtttaagat
     9241 catccgcggt gtacgtcagc ggcccgactt gcctcacgtg gtcgcccact cggttggagg
     9301 gtgggcgaca gtcgtaccgc acgggcgtag acgggggaac gccaccgatt cgcaatggaa
     9361 ccgctccccg attgtgtatg cgcgcctcct ttcaagcgcc atctagcagg cggagcattg
     9421 atgcccgggc atttgcggcg aaggaaccgc tggtctggtt caggaggttg tcgtgagcag
     9481 attcaccgag aagatgtacc gcaacgctct gagaaccgac cggggcatgg tgaccggcga
     9541 gccgcacgaa ccggtccggc acacctgggg tgaggtgcac gagaaagctc ggcggatcgc
     9601 cggtggcctg gccgcggccg gcgtcggaca cggcgacgcg gttggcgtac tggccggcgc
     9661 acccgtggag atcgcgccca ccgcgcaggg gctgtggatg cgcggcgcca gcctgacgat
     9721 gctgcaccag cccacgcccc gcaccgacct ggcgctgtgg gcccaggaca cgacgaccgt
     9781 catcgacatg atcgaggcca aggccgtcgt catctccgac ccgttcaccg tcgcgattcc
     9841 ggtgctggaa gaacgcggcg tcaaagtcgt caccgtcgaa cagttgctcg ctgccgaccc
     9901 aatcgacccc gtcgagacgg gggaggacga cgtcgcgctg atgcagctga cgtcgggctc
     9961 caccgggtcc ccgaaagcag tggtgatcac ccaccgcaat gtctactcca acgccgaggc
    10021 gatgttcatc ggcgccgagt acgacgtcga ccgcgacgtg atggtcagct ggctgccctg
    10081 cttccacgac atgggcatga tcggcttcct gactgtcccg atgtacttcg gcgccgagct
    10141 ggtcaaggtc accccgatgg acttcctgcg cgacacgctg ctgtgggcca agctcatcga
    10201 caagtacaag ggcacgatga cggccgcgcc gaacttcgcc tacgcgctgt tcgcgaagcg
    10261 gctgcgcaag caggccaagc ccggccagtt cgacctgtcg acgctccggt tcgcgctgtc
    10321 gggctccgag ccggtcgaac cggccgacgt cgaggacctg atcgacgccg gtaggccgtt
    10381 cgggctgccg gcggaggcga tcctgccggc ctacggcatg gcggagacga cgctggcggt
    10441 ctcgttctcc gagtgcggcg ccgggctggt ggtcgacgag gtcgacaccg acctgctggc
    10501 cgcgctgcgc cgcgcggtgc cggccacgaa gggcaacacg cgccgcctgg cgtcgctggg
    10561 gccgctgctg aaggacctcg aggcgcgcat cgtcgacgag gacggcaacg tgctgcccac
    10621 ccgcggcgtc ggcatcatcg agctgcgcgg cgaggcgctg acgcccggct acatcacgat
    10681 gggcgggttc gtcccggcgc aggacaacca cggctggtac gacaccggcg acctcggcta
    10741 cctgctggag aacggtcacg tcgtggtctg cggccgcgtc aaggacgtca tcatcatggc
    10801 cggccgcaac atctacccga ccgacatcga gcgggcggcc tcccgcgtcg acggcgtccg
    10861 gcccggctgc gcggtcgcgg tgcgacttga cgccggccat tcgcgagaga cgttcgcggt
    10921 cgccgtcgag agcaacgcct gggaggatcc ggccgacgtg cgtcgcatcg agcaccaggt
    10981 cgcccacgag gtcgtcgggg aggtcgacgt gcggccccgc aacgtcgtcg tcctcggccc
    11041 cggcacgatc cccaagacgc cgtcgggcaa gttgcgccgc gccaactcgg tcgccctggt
    11101 cagctagccg cccgctcacc ttcccgcgag cgtgcgtgtc tgtacctcga cacgccgctc
    11161 tagcacgtac atctgcgcac gctcgcgcca cgacgagcgc gccaagtttc cgttgaggat
    11221 tcccagaggg ccgggttcga gccttttctc atcaccccac gccgctgcgg cggcaacgag
    11281 aaaaggacaa cccacatgag caataccttc ttcacacgga ttgcagccgc agcattcatc
    11341 ggcctcgcaa cggtcggtgc caccggcgcc accgtgtcgt ttgccggccc cgcgatggcc
    11401 gccccggccc cgtccgtggg ccccagcacc accggcatgt acggcgaccc gaccgaggcc
    11461 gccgagttct gggccgagca gagcctggac gactgcacgc tgatggcgat cgcgagcgtg
    11521 gtcggccagg tcaccgggga tatgcccacc gaggcggaga tcatcgagct ggcggagaac
    11581 accccgtcca ctacccaccc tggctcgatc tacatccggc cggccaatcc ggaggatccc
    11641 aacaccggga acgggaccga cccccacgac gcggtcgtgc tgctggcgca ctacggcatc
    11701 gacgccgagg tcaccgacgc cgacagcgaa gacgaggctg gcgtcgacac cggcatgcgg
    11761 gctctcgagc agtatctggc cgccggccac aaggtgatcg tcagcgtcaa cgccgagacg
    11821 atctgggaca tcgacggccc gcgcaatcag tccaaccact ccgtctccgt gatcggcgtc
    11881 gacgtcaaga acggcatcgt gcacctcaac gacagcggca ccgaggacgg ccgcgacgag
    11941 caggtcgaca tcgccacgtt catgaaggcc tggaagacca gcgactaccg gatgatcgtc
    12001 accgaggaag ccgcctaaca accctgcccg gcacgcccag cgtgagccca ctgcgaaatt
    12061 tcgagccgat tttcgcagtg ggctcacgtt cgcgtgaggt gggcccgcgg cgcttccaag
    12121 acttcattga ggatctgtaa agggccgcgt tcgacccttt ctcgagagcc catcgccggc
    12181 atcgagaaaa ggacagcccg cagtgaacac cacgttctcc acccgcatcg cccacgcggc
    12241 acgcaccgct gtcgtggccg ttgtgatcgg cgcgtccggc gccgccggca tcgcttgcgc
    12301 ctccgtggcc aacgcctccc cggtcatcga ggtcaccact cccgacggcg tctacggcgc
    12361 ccccgacgcg gcggcggagt tctggcaacg gcagcacctg agcgacgact gcgtcctgat
    12421 ggcggtcgcc gacgtggtcg gccagatcac tggcgacctg ccctcagagc aggaaatcct
    12481 cgaggtcgcc gctgacacca agagcaattc acatagcggg cccgtctacc tgcccgacga
    12541 caaccggtgg ggcaccgatt acggggacgc cgaggtgctg ctcgccgagt acggcatcac
    12601 cggcacgatg accagcacgg ccatcgacga gcagaccggg gtggagaccg gcatggacgc
    12661 gctcatcgag tacttggcca caggggagaa ggtgatcgtc ggcctcaacg gaccgacggt
    12721 ctgggacacc tccggcgacc gcaagaagcc ggcccacgcc gtggtggtga cgggagtgga
    12781 caccgtgcac gacgtggtgc acctcaacga cccctacccc gccgacggcc gcgacctgga
    12841 ggtgcccttg gccaccttcg agaaggcatg gcgcacaggg ccattcatca tgatcgttac
    12901 cgacgaagcc gcgtaaccgc cataggctta ccggagcggg acttgcggtg cgggtcgagt
    12961 cgtgacagcg ctgcgacgag cagcgccacc atcaccaccg ccaacacgag gcacatcccg
    13021 aacgcggtca gccacgcgcc gagggtgtga cgccacagcg ggtcgtcggt gcccgggctg
    13081 gagatcgtgt tgaggttgac ggtcgctgca cccatggcgt aggcccaccg cgacggtgac
    13141 aaccacgaca gctgccccat cggcacctgg tcgcgaaccg ggaacaagcc gccgcacagg
    13201 atcagctgcg ccatcaccac cagcacgagc agtggcatcc cgcggtcggc gttgtcgatc
    13261 atcgccgaga tcagcaagcc gaccaccatc gacaccacgc tgatggtcgc cactgcgaac
    13321 gcgacttcca acctggcgga agggatcagc agaggctcgt cgggaccggg taagaagacc
    13381 actccgagca caccgagcgc gaggccttgc aggccggtga gcatcgtcag gatcaccagc
    13441 ttggaggcca agtacgcgcc gcgcgacagg ccgatcgtgt gttcgcggcg atagatcgcc
    13501 tgctctttga cgatctcgcg aattgaggcg gcgcagccca tcagtgcgcc gccgatgatc
    13561 agcagcacca gcagctgccg tggttgctgg gaatgctgcg cgatgcctcg cagcaccgaa
    13621 agcccggcgc tgcccggaac ggcatacaag aatatgctca gcagcagcgg cagcgcgagc
    13681 atgaacaccg agtactgccg gtcaccggcg atgaccgaca ggtaccgccg gcacagtatt
    13741 gcgaactggt cgaacgggct ctgctgcgcg atggccggca gtggcttggc aggagcagcg
    13801 gaggccacag cttgccgccc aaccggcggg gtggcgggcc tgatgtaggt cccgtaaaaa
    13861 ggggagttcg cgaggcggcc ggcccagttg gtggtggtgt cgcgttcgag cagcgtgaac
    13921 aggtcgggaa agtcgacgca accgaaatac tcgagcgcct gcgtgggcgg gccga
//
LOCUS       DASWBF010000462         9078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1031012,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000462 DASWBF010000000
VERSION     DASWBF010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031012"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1281
                     /locus_tag="VF874_16930"
     CDS             31..1281
                     /locus_tag="VF874_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177683.1"
                     /translation="MTETTSALTTESVAEFAARARAWLADNMPRIDPAQPPRAERGEQ
                     GPWKRARELQKRLYEGGFAGICFPREYGGHGLPIEYQRAFDVESRCYELPIILNTPTF
                     TICCATILDTGSEEQKRQHISAAIRGDEVLVQLLSEPSGGSDLAGVITRADRVGDKWV
                     INGAKTWSTSAFAADYGLLLARTDWDVPKHEGLTMFVVPINSPGITLRRIKQVNGSQE
                     FCEEFFDGLELDDGAVIGEVNKGWDVASRQLYHERRAVGGGSEFASGIGAEGTTDVPV
                     DFAALLEALGQADNERLQEMAGRALVHRAVQEQLIDHVYHGVNDGSLPPAAGTIIRIS
                     HSENIQLEMDTALAIAGSAGVVDGGDGLIRFGERFLSRQTASLGGGTTEIGRNVIGER
                     VLGFPREYAADRGVPFKDVKHNRS"
     gene            complement(1317..1745)
                     /locus_tag="VF874_16935"
     CDS             complement(1317..1745)
                     /locus_tag="VF874_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HEX9177684.1"
                     /translation="MQNRIAVAAAAALVVAGLAACSSDKQPSHPSQASVTINGDTVAN
                     QQAVTCNQEQWYWTIAIGDQKVSGVKALVNKTDDKFTAQSVHIVNLGGFTGTYAKGDG
                     EDASASFSSETFTLTGTAKGFNTYTPNESATATFKIVATC"
     gene            complement(1786..3033)
                     /locus_tag="VF874_16940"
     CDS             complement(1786..3033)
                     /locus_tag="VF874_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9177685.1"
                     /translation="MLIRQATLLDGRVADIRLGEHITDVAPTLTRHVGETVVDARLGA
                     VLPGLHDHHLHLRAAAAALGSVVVGPQFTQAIAGAEPDPDGWIRAVGYHESVAGELDR
                     VSLDAVRPDAPLRVQHRSGALWILNSAALARIDCADHPDGRIPSTDPRLDAIPRRVPD
                     LAALGRQLARYGVTGVTDATPDLQPDDIASLTAAFKQRVRFLAPGKKILHDQHLDLDG
                     LTEWIAECHADNVPVAIHCVTAAQLIVAIAALRTAGGHPWDRVEHAAMVPDVTELAEL
                     GVTVVTQPNFVAERGEQYLTDIPADEHAQLWRVASLIRAGIPVAGSTDAPFGALDPWA
                     AMRAAVTRATASGHVIGPRERVEPLQALQMFLGAADRPAQPRCIAPGQPGDVCILAVP
                     PDEALRALASDMVVAAVVAGAIA"
     gene            complement(3027..4136)
                     /locus_tag="VF874_16945"
     CDS             complement(3027..4136)
                     /locus_tag="VF874_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9177686.1"
                     /translation="MASWGLPVSVLSRAREVAAVIEDRVDSLGGRTLRVDADEIVTGR
                     AALLGLDPPSRISSGGASRLLATADGWCAFTLSRVEDYEALPALLEIGDVGADPWDSL
                     AAAARARKAEEIVARARLLDMPAAVLGEAVASDAKIVRHSAPLSRSITDLLVVDLSSM
                     WAGPLCGRLLGAAGATVVKVETPGRPDGTRGGHPHFFNWINSEKLCYSDDFAQLLRAA
                     DVVIEGSRPAALARRGLGAEQVPGRPGRIWLRLSGYGAIHPDWVAFGDDAAVAGGLVT
                     FRGDCPVFCGDAIADPLSGLAATAAILDALASGGGVIIDVAMSAVAAAYAGLPDAGVE
                     RTQLRAPALPSEPAHELGADNAHVRELIESRLAAC"
     gene            complement(4141..5121)
                     /locus_tag="VF874_16950"
     CDS             complement(4141..5121)
                     /locus_tag="VF874_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020107126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9177687.1"
                     /translation="MNLDPDSASSLQLRIIDLAATVAMDLDVPAGPVVAVGSAADIRA
                     WQPWVEQATFTLTTDASHDRRVVTVRSIDAALQNLAERVEHSPQAAAICDDVLRSVDP
                     TAPARSGLITESLAYSTLQSGREFADWLARRGPATVPRTPDPVLLERQGDSLAVWLNR
                     PVRHNAFSTDMRQALLDALAVANCDASIRQVVLAGKGPSFCSGGDLAEFGSFDDPATA
                     HFARTRHSPALALDELSTRFGAQLIARVHGQVLGSGLEMAAFCRWVEAHPESRFGLPE
                     VALGLIPGAGGTVSITRRIGRWRTAYLVLSGQTLTAATARAWGLVDAVTQ"
     gene            5785..6000
                     /locus_tag="VF874_16955"
     CDS             5785..6000
                     /locus_tag="VF874_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX9177688.1"
                     /translation="MEKTTIYLPGDLKTAVKHAARQRGVSEAEVIRQSIRAGVGGVKP
                     RPRGGLYSGSKPIARRVDEFLDGFGER"
     gene            5997..6404
                     /locus_tag="VF874_16960"
     CDS             5997..6404
                     /locus_tag="VF874_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9177689.1"
                     /translation="MIVDTSALLAYFDSDEPDHDAVANVIERSEESLVVSPYVVAELD
                     YLVATRVGIDAELAVLRELSTGAWELAAFGVPELEQAASIVRKYRDQHIGVADASNVV
                     LADRHHTRTILTLDRRHFTVLRPLRGGRFAVVP"
     gene            complement(6466..6552)
                     /locus_tag="VF874_16965"
     tRNA            complement(6466..6552)
                     /locus_tag="VF874_16965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6516..6518),aa:Leu,seq:tag)
     gene            6870..7196
                     /locus_tag="VF874_16970"
     CDS             6870..7196
                     /locus_tag="VF874_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEX9177690.1"
                     /translation="MPAATARSRQKHTRPIQLERWQQALVNEATEDFIRGLIHSDGCR
                     VVANDRGVKSVRYHFSNRSEDILGLFTAALDDLGIPWTRSTKYIVSVYRKAATARLDE
                     FVGPKC"
     gene            7213..7587
                     /locus_tag="VF874_16975"
     CDS             7213..7587
                     /locus_tag="VF874_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177691.1"
                     /translation="MSTTRRRRPALIVLVIVAAAACLALGWWQWQRFESTSGTGQNLG
                     YALQWPLFAAFVVYAYRKFVRYEEEPPQLHQPDTVTEIPAGLLPERPKPTPEASQDPT
                     LAEYNAYLAQLAETDKKMRQEH"
     gene            7588..7923
                     /locus_tag="VF874_16980"
     CDS             7588..7923
                     /locus_tag="VF874_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HEX9177692.1"
                     /translation="MSSETPTDTSKIRTALTAYRVMAWATGIWLIALCYEVVVKYVVQ
                     VENPPSWIGVVHGWVYFVYLLATLNLAVKVRWPLWRTVGTLLAGTIPLLGIILEHFRT
                     KQVKKAFDL"
     gene            7821..>9078
                     /locus_tag="VF874_16985"
     CDS             7821..>9078
                     /locus_tag="VF874_16985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9177693.1"
                     /translation="MAHGRHSARRHDPAPRHHSRALPHQAGEESIRPVAALAAIVVAG
                     LVLRTFVWWPSNNLFGVLEYDDGVYYAAARLLLDGYLPYSDFTIVHPPGMSLVLLPAA
                     GLGYLFGDPVGMVAGRIEIQLIAAINIVLVHRLASRLPGDPATRNRRALVAAALYAVM
                     PNAVVAEQTILLEPLATCACLLAMWFLLRRDNPSRFDVITSGFFLVTAVALKLFAAAY
                     LVAVVGYLLWTRRPKSLLALAGGGLLATAVVIAPLVISAPINAWHDVVVAQFSRPQNP
                     FVPDGLDRLIDMVGLGAVGIPVALVLIVLLSIATVPHFSPQVDLWVSVLIMIGCAFAT
                     SPTYFTHYGESLGPAVTLLVSRSLDIRRGALLVTAFAVAFATGTGVHLSDLRGQGDLR
                     AATAEIPNGSCVYFDAISLPLMADVYT"
BASE COUNT         1568 a         3012 c         2939 g         1559 t
ORIGIN      
        1 cgcgttgacc aaacagctgg agtccgcctc atgaccgaga cgacgtcggc gctgaccacc
       61 gaatcggtgg ccgaattcgc cgcgcgggcc cgggcatggc tggcggacaa catgccacgc
      121 atcgatccgg cccagccacc acgggccgag cgcggagaac aagggccctg gaaacgggcc
      181 agagagctcc aaaagcggct ctacgagggc ggattcgcag gcatctgctt cccgcgggaa
      241 tacggcggcc acggtctgcc catcgagtac cagagggctt tcgacgtcga aagccgctgc
      301 tatgagctgc cgatcatcct caacacaccg acgttcacga tctgctgcgc gacgatcctc
      361 gacactggca gcgaagagca gaagcgccaa cacatttcgg ccgcgatacg cggtgacgaa
      421 gttctcgtgc agttgctgtc ggagcccagc ggcggatcgg atctggccgg cgtcatcacc
      481 cgggccgatc gcgtcggaga caagtgggtg atcaacggtg cgaagacgtg gagcaccagt
      541 gccttcgccg ctgactacgg actgctgctg gcccgtaccg attgggacgt gcccaagcac
      601 gagggcttga cgatgttcgt ggtgccgatc aacagccccg gcatcacgtt gcggcggatc
      661 aagcaggtca atgggtcaca ggagttctgc gaggagttct tcgacggcct cgaactcgac
      721 gacggcgcgg tgatcggcga ggtcaacaag ggctgggacg tggcgtcgcg tcagctctac
      781 cacgagcggc gcgcggtcgg cggcggctcg gagttcgcca gcggcatcgg tgccgagggc
      841 accacagacg tgccggtgga ctttgcggca ctcctcgagg cgctgggcca ggccgacaac
      901 gaacgcctac aggagatggc cgggcgggcg ctcgtgcacc gtgcagtgca ggagcaactg
      961 atcgaccacg tctatcacgg cgtcaacgac ggctcgttgc cgccggccgc gggaaccatc
     1021 atccgcatct cacactccga aaacatccag ctcgagatgg acaccgcgct ggcgatcgcg
     1081 ggcagtgcgg gcgtggtcga cggcggcgac ggcctgatcc gcttcggtga gcgtttcttg
     1141 tcccggcaga ccgcgtcgct gggcggcggc accaccgaga tcgggcgtaa cgtcatcggc
     1201 gagcgggtac tgggctttcc gcgggaatac gcggccgacc ggggcgtgcc cttcaaggac
     1261 gtgaagcaca accgcagctg acgtggacga tggtcgctcg gacgagcctc gctccgtcag
     1321 caggtcgcga cgatcttgaa cgtcgcggtg gccgattcgt tcggcgtgta ggtgttgaaa
     1381 cccttcgcag tgccggtgag cgtgaacgtc tccgaactga agctggctga ggcgtcctca
     1441 ccgtcgccct tcgcgtaggt gccggtgaag ccacccaggt tgacgatgtg gaccgactgg
     1501 gcagtgaatt tgtcgtcagt cttgttgacc agcgccttga cgccggagac cttctggtcg
     1561 ccgatcgcga tcgtccaata ccactgctcc tgattgcatg tcaccgcctg ctggttcgcg
     1621 acggtgtctc cgttgatcgt caccgacgcc tggctggggt ggctcggctg cttgtctgag
     1681 gaacacgcag cgagtccggc gacgacgagc gccgctgcgg ccgcgacggc gattcggttc
     1741 tgcacttggg cagaatacaa ttctccgatt tcgattgtcg ggttatcacg caatggcgcc
     1801 cgcgacaacg gcggcgacca ccatgtcaga ggccagagcg cgcagcgcct cgtcaggcgg
     1861 caccgccagg atgcacacgt cacccggctg tccgggcgcg atgcaccgag gctgtgcggg
     1921 gcggtctgcg gcgccgagga acatctgcag tgcctggagg ggctcgacgc gctcgcgtgg
     1981 gccgatgacg tgccctgacg cggtggcacg cgtgacggcg gcgcgcatcg cggcccacgg
     2041 gtcgagggca ccgaacgggg cgtcggtact cccggccacc gggatgcccg cgcggatcaa
     2101 cgacgccacc cgccacagct gcgcgtgttc gtcggcgggg atgtcggtca ggtactgctc
     2161 gccgcgttcg gcgacgaagt tcggctgggt cacgacggtc acgccgagtt ccgcgagctc
     2221 ggtcacgtcc ggcaccatcg cggcgtgctc gacccggtcc catgggtggc cgccggcagt
     2281 gcgcagtgcg gcgatggcga cgatgagctg ggctgccgtg acgcaatgga tcgcgacggg
     2341 cacattgtcc gcgtggcatt ccgcaatcca ttcggtgagc ccgtcgaggt cgagatgttg
     2401 gtcgtgcagg atcttcttac ccggcgccag gaatcgcact cgctgcttga acgctgctgt
     2461 gagagaggcg atgtcgtcgg gttgcaggtc cggcgtggcg tcggtgacac ccgtgacgcc
     2521 gtagcgcgcg agctggcggc caagcgccgc aagatccggt acccggcgcg gtattgcgtc
     2581 caatcgagga tccgtgctcg gaatccgtcc gtcgggatgg tctgcacaat cgatgcgcgc
     2641 aagcgctgcg gaattgagaa tccacagcgc gccgctgcgg tgctggaccc gcagcggcgc
     2701 gtccggccgc acggcgtcga ggcttacgcg atccaactct cccgccaccg actcgtggta
     2761 gccgactgcg cggatccacc cgtcgggatc gggctccgcc ccggcgatcg cctgggtgaa
     2821 ctgcggtccg acgacgacgg aaccgagcgc cgcggcggcc gcccgcaggt gcaggtgatg
     2881 atcatgcagt cccggcagca cggcgcccaa ccgcgcgtcg acgaccgtct ctccgacgtg
     2941 acgcgtgagc gtcggggcca cgtcggtgat gtgctcgccg aggcggatgt cggcgacgcg
     3001 cccgtcgagc agagtcgctt gtcgaatcag catgcggcca gccgggattc aatcagttcg
     3061 cggacgtggg cattgtcggc gcccagctcg tgggccggct ccgacggaag cgcaggtgcg
     3121 cgcagctggg tacgttccac tccggcatcc ggaaggccgg cgtatgcggc ggcaaccgcc
     3181 gacatcgcga cgtcgatgat gacgccgccg cctgatgcca gggcgtcgag tatggccgca
     3241 gtcgccgcca gcccgctgag gggatcggcg atcgcgtcgc cgcagaacac cggacagtcc
     3301 ccgcggaacg tcaccagccc accggccacc gcggcgtcgt cgccaaaagc gacccagtcg
     3361 ggatggatgg ccccgtatcc gctgagacgc aaccatattc gtccggggcg ccccgggacc
     3421 tgctcggcgc ccagtccgcg acgggcgagc gcagcgggcc gggatccctc gatcaccaca
     3481 tcggcggccc gaagcagctg tgcgaaatcg tcggagtagc agagcttttc actgttgatc
     3541 cagttgaaga aatgtggatg gccgccgcgg gtgccgtctg ggcggccagg cgtttccacc
     3601 ttgacgacgg tcgcacctgc ggcgccgagc aaacgcccgc agaggggccc agcccacatc
     3661 gacgacaggt cgaccacgag cagatcggtg atcgaccgcg acaatggtgc gctgtgccgc
     3721 acgatctttg catcactggc aacggcctcg ccgagcaccg cggcgggcat gtccagcagc
     3781 cgggcccgcg ccacgatctc ctcggccttg cgtgcccgcg cagcggcggc cagagagtcc
     3841 cacggatcag cgccgacgtc gccgatctct agcaacgccg gaagggcttc gtagtcttcg
     3901 acccggctca gcgtgaatgc gcaccagccg tctgcagtag cgagcaatcg gctggcgccg
     3961 cccgaggaga tgcggctcgg cgggtcgagg cccagcagtg cggcacgtcc ggtgacgatc
     4021 tcgtcggcat ccacccggag agtgcggccc ccgagtgagt cgacacggtc ctcgatcacg
     4081 gcggccacct cacgagcgcg cgacagcacg ctcaccggaa gaccccatga cgccatggct
     4141 ttattgtgtg acggcgtcaa ccaagcccca cgcccgggcg gttgcagcgg tcagcgtctg
     4201 cccggacaag accagatatg ccgtgcgcca ccggcctatt cgccgggtga tgctcacagt
     4261 gccgccggcg ccggggatca ggccgagtgc cacttcgggc aatccgaacc gggattcggg
     4321 gtgcgcttcc acccagcggc agaacgcggc catttcgagt ccgctgccca gcacttgacc
     4381 gtggacgcgg gcaatcagct gtgcgccgaa ccgcgtcgac agttcgtcga gtgccagggc
     4441 cgggctgtgg cgggtgcgcg cgaagtgggc ggtcgccgga tcgtcgaacg acccgaattc
     4501 ggccaggtca ccgccgctgc agaacgacgg gcccttccct gcaagtacca cctggcgaat
     4561 cgacgcatcg cagttcgcga cagccagcgc gtcgaggagc gcttggcgca tgtcggtgga
     4621 gaacgcgttg tgccgaaccg gccggttcag ccacactgcg agcgaatcac cttgccgctc
     4681 aagcagaacc gggtctggcg tacgcggcac ggttgccggt ccgcgacgcg ccaaccagtc
     4741 ggcgaactcg cgaccagact gcaacgtcga gtaggcaagc gactcggtga tcaggccgct
     4801 tcgagctggc gcggtgggat cgaccgaccg caacacgtcg tcgcagattg ccgccgcctg
     4861 cggcgagtgc tccacgcgct cagcgaggtt ctgcaacgca gcgtcgatcg accgaacggt
     4921 gacgacgcgg cgatcatgtg acgcgtccgt ggtcaatgtg aaggtggcct gctcgaccca
     4981 tggctgccaa gctctgatgt ccgccgcaga acccaccgcg accaccggcc cggccggtac
     5041 gtcgaggtcc atggcgacgg ttgccgccaa gtcgatgatc ctcaactgca gtgaggatgc
     5101 gctgtccgga tcaaggttca tcgccgcgaa catacgacgt cgcggccgcg aatgtgaccg
     5161 gatggcttag ctccacgttc tgagcatgcg aatgcgcgct ttacggcagc ccactcggcg
     5221 gccgtcggct gttggctccg gttctctatt gcgcatgaac tgacctccgg ccccggcgcg
     5281 aggggcgaaa gcatcgttgc gatcttcatc acctgcccag ctggagtctc ttcgaagttc
     5341 tcgaaaattc tcttttggag ccaactttcg acctcatcga gtccagctgc cacatcaagc
     5401 tcagggaccg tcaccacatc gttgcagtgc gccaggtgtt cttcgtgccc gtcgccccag
     5461 cactctgtcc gccagccggg ccagagatca ggagcttggg caggaaccgg cgcaccggtg
     5521 ccgggccgcc ctgcaacccg acgtgggaga agaacagttc acggctgggc ggcaacacag
     5581 gaccggatcg gcgacgaggt gcacagcgac ggcaccgcgg tctgagcctc tcggtgacca
     5641 gagttcatgt gaaagcgacc atgtcgcaac tcacctcggc ttggcggtca aattactgtt
     5701 tagatcagcg tcagggatac gccgtcgccg agcgccggac tcacggaggt aatatagggt
     5761 actagcggtg gtaccatata gatcgtggag aagacgacga tctatttacc tggggacctc
     5821 aagacggcgg tcaagcacgc tgcccgacag cgcggagtct ccgaagctga ggtcatccgg
     5881 caatctattc gggcaggcgt cgggggcgtc aagccacggc cacgtggtgg tctctactca
     5941 gggtccaagc cgatcgcccg gcgtgtcgac gagttcctcg atggctttgg tgagcggtga
     6001 tcgtcgacac cagtgccctg ctcgcatatt tcgattccga cgaacccgac catgacgccg
     6061 tggccaatgt catcgagcga agcgaggagt cgcttgtcgt ctcgccatac gtcgtcgcag
     6121 aactcgacta tctggtcgcc acccgcgtcg gcatagacgc ggagctcgcg gttcttcgtg
     6181 aactctcgac gggtgcatgg gaactggctg ctttcggtgt gcccgagctg gagcaggccg
     6241 cctcgatcgt cagaaagtac cgtgaccaac acatcggggt ggcggacgcg tccaatgtgg
     6301 tgctggccga tcgccaccac acccggacaa ttctgacgct cgaccgtcgt catttcaccg
     6361 tgttgcgtcc acttagaggg gggcggttcg ccgtcgtgcc gtgaggcaat tgtgcagcag
     6421 gcagctgcgg aaggcgacgg tgacggtcga ggactgggca gcgcgtggtg cgggcggagg
     6481 gactcgaacc ctcatgctct ctcgagcacc ggcacctaaa gccggcgtgt aagccagttc
     6541 caccacgccc gcggccagcc gatcgtaccg ccctgcacgc ccacgaacct gtcggtgatg
     6601 cgcactacgt tgagccacaa cgcgaggagt gatttctcgg cccttccggc gaaggcgtac
     6661 agctatctgc tcggtctgtt cctgggcgat ggatgcatct cacgacaccc tcgggcgtgg
     6721 cgcctgcgga tcactctcga caagaaatac ccaggaatca tcgaccgctg ccgcgaggcc
     6781 atcgacaccc taatgccgga gctacgcgca tcgatcgagc cacaaccgaa gggatgcgtc
     6841 gttgtttcgc tctactccaa gcactggccg tgcctgctgc cacagcacgg tcccggcaga
     6901 agcacacgag gcccatccaa ctcgagcggt ggcagcaagc tctcgtcaac gaggcgaccg
     6961 aagacttcat ccgcggcctg attcacagcg acggatgccg cgtggtcgcc aacgatcgcg
     7021 gcgtcaagag tgtccgctac cacttctcca acagatcaga agacatcctc ggactgttca
     7081 ccgccgcgct cgacgacctc ggcattccat ggacaagatc gaccaagtac atcgtctcgg
     7141 tctaccgcaa ggcggcgaca gcacgcctcg acgagttcgt cgggccgaag tgctgagcgg
     7201 taccgtcgag gcgtgtccac tacacggcgt cgcagacccg cactcatcgt gctggtcatc
     7261 gtcgccgccg cggcatgcct tgcgcttggc tggtggcagt ggcaacgctt cgaatcgacg
     7321 tcgggcaccg gccagaacct cggctacgcg ttgcagtggc cgctcttcgc ggcgttcgtc
     7381 gtttatgcct accgcaagtt cgtccgctac gaggaagagc cgccgcagct gcaccagccc
     7441 gacaccgtca ccgagatccc ggcgggcttg ttaccggaga gaccgaagcc aacgcccgag
     7501 gcatcccaag accccaccct cgccgagtac aacgcctacc ttgctcaact tgccgagacc
     7561 gacaagaaga tgcgacagga acactagatg tcatccgaaa ccccgacgga cacaagcaaa
     7621 atccgcaccg cgctcaccgc gtaccgggtg atggcctggg ccacgggcat ctggctcatc
     7681 gcgctctgct acgaggtcgt tgtgaagtac gtcgtccagg tcgaaaatcc gccgtcctgg
     7741 atcggcgttg tgcacgggtg ggtgtacttc gtgtatctgc tcgcgacgct caacctcgcc
     7801 gtcaaggtcc gctggccgct gtggcgcacg gtcggcactc tgctcgccgg cacgatcccg
     7861 ctcctcggca tcattctcga gcacttccgc accaagcagg tgaagaaagc attcgacctg
     7921 tagcggcgct ggcggcgata gtcgtcgccg gcctggtgct gcgcacgttt gtgtggtggc
     7981 cgtcaaacaa tctgtttggc gttctcgagt acgacgacgg cgtctactac gccgccgcgc
     8041 gactgctgct cgacggttac ctgccttata gcgacttcac aatcgtccac ccaccgggga
     8101 tgtcactcgt tctcttgccc gccgcgggcc tcgggtatct cttcggcgat ccggtcggaa
     8161 tggtcgcggg ccgaattgag atacagctga ttgctgccat aaatatcgtg ctcgtgcacc
     8221 ggctcgccag ccgccttccc ggcgatccgg cgactcgcaa ccggcgtgcg ctcgtcgcgg
     8281 ctgccctcta cgccgtcatg ccaaacgccg ttgtcgccga gcagaccatc ctgctcgagc
     8341 cgcttgccac ctgcgcgtgc ctcctcgcga tgtggtttct cctgcgacgc gacaatccgt
     8401 cacggttcga cgtcatcacg tccggctttt ttctcgtcac cgcggtcgcg ctgaagcttt
     8461 ttgccgccgc gtacctcgtc gcggtcgtcg gctatctact gtggacacgc cgaccaaagt
     8521 cgctcttggc tctcgcgggc ggcgggctgc tcgctacggc tgtcgtcatc gcaccacttg
     8581 tcatctcggc tccaatcaac gcctggcacg acgtcgtcgt cgcccaattc agccggccgc
     8641 aaaacccgtt cgtgccggac ggtctcgatc gcctcatcga catggtcggt ctcggggccg
     8701 tggggatccc ggttgccctc gtgctgatcg ttttactctc catcgcgacg gtgccgcatt
     8761 tcagtccgca agtcgacctg tgggtctcgg tgttgataat gatcggctgt gcattcgcaa
     8821 cctcgccgac gtacttcacg cactatggcg agtccctcgg tcccgcggtc actctcctcg
     8881 tgagtcgatc actcgatatt cgacggggag cactgctcgt cacagcgttc gctgtcgcat
     8941 ttgcgaccgg aaccggcgtc cacctcagcg accttcgggg ccaaggcgac ctgcgcgccg
     9001 cgaccgctga aattcccaac ggcagttgcg tctacttcga cgcgatcagc cttccgctca
     9061 tggccgatgt ctacaccg
//
LOCUS       DASWBF010000463         5240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1232374,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000463 DASWBF010000000
VERSION     DASWBF010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232374"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VF874_16990"
     CDS             <1..398
                     /locus_tag="VF874_16990"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEX9177694.1"
                     /translation="PIDCGAYTYDADDDYPAFCISAAERTVKDPGSLGIVIGGSGNGE
                     QIAANKVPGARCALGWNTETASLAREHNDAQLIGIGARMHTVEQALAIVDAFLSTPWS
                     KAERHQRRIDILAEYERTHQAPPVPGAPD"
     gene            402..1196
                     /locus_tag="VF874_16995"
     CDS             402..1196
                     /locus_tag="VF874_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX9177695.1"
                     /translation="MPEGHTLHRLARLHQRKFSGHPVAVSSPQGRFDDGAALVNGRVL
                     RRASAYGKHLFHHYAGGPIVHVHLGLYGAFTELAPPMPLPVGEVRMRMVGVDFGTDLR
                     GPTACEVLDEAQVDAVIARLGPDPLRRDADPAPAWKRIAKSQRPIGALLMDQAVIAGV
                     GNVYRNELLFRHGIDPYRPGTAVDAPEFDSAWADLVELMRVGYRRGKIHVIRKEHDRG
                     APSYRPGRPRTYVYRRAGDPCRVCGTKVLTAVLEGRNVFWCPTCQT"
     gene            1320..1691
                     /locus_tag="VF874_17000"
     CDS             1320..1691
                     /locus_tag="VF874_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX9177696.1"
                     /translation="MTSEERDRDDPGAEPDYRFTLANERTFLAWQRTALGLLAAAVAI
                     VQFVPQFGIPGARHIVAAFLTILAILSAGLGLRRWRLVDDAMRRGQPLPRQSAPVFLG
                     LGLVLLGAVILGIVVVKAVIQ"
     gene            1688..1996
                     /locus_tag="VF874_17005"
     CDS             1688..1996
                     /locus_tag="VF874_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX9177697.1"
                     /translation="MTSPGPEGLQAERTALAWSRTSLAVMVNGVLLVLKEPRNDARPI
                     AAALAALIAVTVYLVGVRRQRTLARRPLPERVTARAQVQLVGVAVLVLIVVTVLGLAL
                     "
     gene            complement(2004..2867)
                     /locus_tag="VF874_17010"
     CDS             complement(2004..2867)
                     /locus_tag="VF874_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEX9177698.1"
                     /translation="MNAALIVILILVVAAIALAVVSSSRASRNRTAASLADAKADARR
                     VIERLGGQVLNLTGTDDASKQAMADASERYTAAGSQIDQATTAKQAQLAKESALEGLY
                     YVRAARQAMGMDPGPELESLTGQQSAGVVTEDRRVQVEGREIEASPTPSPRTPNYFPG
                     GRVAGRPVPAGWYSEPWWKTALVAGAWGLGSVLLFDALFSGMHGVGYDAAQFENGYGQ
                     GFDSGYDAGLDAGQDSGGWDSGVGDSGGWGDSGGWDSGVGDSGVGDSGGWGDSGGSDF
                     GGGDFGGFGDF"
     gene            complement(2963..3033)
                     /locus_tag="VF874_17015"
     tRNA            complement(2963..3033)
                     /locus_tag="VF874_17015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2999..3001),aa:Gly,seq:tcc)
     gene            3123..3199
                     /locus_tag="VF874_17020"
     tRNA            3123..3199
                     /locus_tag="VF874_17020"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3157..3159,aa:Pro,seq:tgg)
     gene            3233..4603
                     /gene="tig"
                     /locus_tag="VF874_17025"
     CDS             3233..4603
                     /gene="tig"
                     /locus_tag="VF874_17025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX9177699.1"
                     /translation="MKSTVEQLSPTRVRINVEVPFTELQPDFDRAYKQLAQQVRLPGF
                     RPGKAPAKLLEARIGRGAVLEQVVNDALPIRYSEAVTSSDVQPIGQPEIEITKIEDGD
                     ELVFTAEVDVRPKIELPELDAVTIEVDPVEVTDEDVDAELESLRARFGTLTGVERAAQ
                     TGDFVSIDLSATVDGQEVPEAATEGLSHEVGSGQLVEGLDDAVVGLSAGESRVFTTTL
                     AAGEHAGQSAQVTVTVNSVKERELPEPDDEFAQLASEFDTIDELRASLGDQVARIKRI
                     QQAEKIRDAAIETLLGQVEVPLPEAIVQAQVDETVHNALHGLDHDEERLAEALAEQGS
                     SREQFDADARENAEKAIKTQLLVDAIADKLEVQVGQDDLTERLVLQSRQYGVEPQQLL
                     QFLQENNQLPAMYADVRRGKTLAAVVRGATVADTAGNTVDTDEFFGPREQAEAEADET
                     TDDEDQ"
     gene            4705..>5240
                     /locus_tag="VF874_17030"
     CDS             4705..>5240
                     /locus_tag="VF874_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX9177700.1"
                     /translation="MRSSAPGLNLVDSVYERLLTERIIFLGSQVDDDIANRLCAQILL
                     LAAEDPTKDIHLYINSPGGSISAGMAIYDTMVLAPCDVATYAMGMAASMGQFLLAAGT
                     KGKRYALPHARILMHQPLGGVTGSAADIAIQAEQFAVIKKEMFRLHAEFPGQPLERIV
                     ADSDRDRWFTAPEALEYG"
BASE COUNT          884 a         1786 c         1788 g          782 t
ORIGIN      
        1 agccgatcga ctgcggcgcg tacacctacg acgccgacga cgactacccg gcgttttgca
       61 tctcggcggc cgaacgcacg gtcaaagacc ccggtagcct gggcatcgtg atcggcggct
      121 ccggaaacgg cgagcagatc gccgcgaaca aggtgcccgg cgcccgctgc gcgctgggct
      181 ggaacaccga gacggcgtcg ctggcccgcg aacacaacga cgcgcagttg atcggcatcg
      241 gcgcgcggat gcacaccgtc gagcaggcgc tggcgatcgt cgacgcgttc ctgtccaccc
      301 cgtggtcgaa agccgaacgc caccaacgcc gtatcgacat cctcgccgaa tacgagcgca
      361 cccatcaggc gccgccggtg ccgggcgcgc cggactagag cgtgccggaa ggtcacacgc
      421 tgcaccggct ggcccggctg caccaacgga agttcagcgg tcacccggtt gcggtgtcca
      481 gcccccaggg ccggttcgat gacggcgccg cactcgtcaa cgggagggtg ctgcggcggg
      541 ccagcgccta cggcaagcac ctgttccacc actacgccgg cggcccgatc gttcatgtgc
      601 acctcggctt gtacggcgca ttcacggagc tcgcgccgcc gatgccgctg cccgtcggcg
      661 aggtgcgcat gcggatggtg ggcgtcgact tcggcaccga cctgcgcggg cccacggcgt
      721 gcgaggtgct cgacgaggca caggtcgacg cggtgatcgc gcggctcggc cccgacccgc
      781 tgcgacgcga tgccgacccc gcaccggcat ggaagcgaat cgccaagtcg cagagaccga
      841 ttggcgcgct gctgatggac caggcggtga tcgccggcgt cggcaacgtg tatcgcaacg
      901 agctgttgtt ccggcacggg atcgacccgt accggcccgg cactgccgtc gacgcacccg
      961 aattcgactc ggcctgggcc gatttggtgg agttgatgcg agtgggctac cgccgcggca
     1021 agattcacgt catccgcaaa gaacacgacc gcggcgcgcc gtcgtaccgc cccggccgcc
     1081 ctcgcaccta cgtctaccgc cgcgccggcg atccgtgccg cgtctgcggc accaaggtgc
     1141 tcacggccgt gctcgagggc cgcaacgtgt tctggtgccc cacctgccag acgtaggctc
     1201 ggcgcagatg gatcgcgtgt cgcacgcact cttcgacgcc tcgagcagga attggaaacc
     1261 gacccccata ttgccgatgc ggtgctgacg atcgccgatc ccgaggatga tgacatgcga
     1321 tgacgagcga ggaacgcgac cgcgacgacc ccggtgctga gcccgactac cggttcacgc
     1381 tcgccaacga gcgcaccttc ctcgcgtggc aacgcacggc actgggcctg ttggcggcgg
     1441 ctgttgcgat cgtccagttc gtcccgcaat tcggcatccc gggagcccgg cacatcgtcg
     1501 cggcgttcct caccatcctc gcgatcctca gcgcggggtt ggggctgcgc cgctggcggc
     1561 tcgtcgacga cgcgatgcgc cgcggccagc cgctgccccg gcagagcgca ccggtgttcc
     1621 tggggctcgg gctggtgctg ttgggcgcgg tgatcttggg catcgtggtg gtgaaggcag
     1681 tgatccagtg acgagcccgg gtccggaagg gctgcaggcg gagcggaccg cgctggcgtg
     1741 gagccgcacg tcgctggccg tgatggtcaa cggggtgctg ttggtgctca aggagccgcg
     1801 caacgacgcg cggccgatcg ccgccgcgct ggccgcactg atcgcggtga cggtttatct
     1861 cgtcggcgtc cgccggcaac gaaccctcgc ccggcggcca cttcccgaac gcgtcactgc
     1921 acgcgcgcag gtgcagctgg tcggggttgc ggtgcttgtg ctgatcgtcg tgacagtgct
     1981 cgggctggcg ctatgacgcc cgctcagaag tcgccgaatc cgccgaaatc gccaccaccg
     2041 aagtcgctgc cgccgctgtc tccccagcca ccgctgtcgc cgacaccgct gtcgccgaca
     2101 ccgctgtccc agccgccgct gtctccccag ccaccgctgt cgccgacacc gctgtcccag
     2161 ccgccgctgt cctgcccggc gtcgaggccg gcgtcatacc cgctgtcgaa tccctgcccg
     2221 tagccgttct cgaattgcgc ggcgtcgtag ccgacaccgt gcattccgga gaacaacgca
     2281 tcgaacagca gtaccgaccc cagcccccag gcgccggcca ccagcgccgt cttccaccac
     2341 ggctcggagt accagccggc cggcaccggc cgcccggcca cccgcccgcc ggggaagtag
     2401 ttcggagtgc gcggcgacgg cgtcggcgac gcctcgatct cacgtccctc gacctggacc
     2461 cgacggtcct cggtgaccac cccggccgac tgctgcccgg tcagcgactc caattcgggg
     2521 ccggggtcca tgcccatcgc ctgccgcgcg gcccgtacgt agtagaggcc ttccagcgcg
     2581 ctctccttgg ccaactgggc ctgcttggcc gtcgtcgcct ggtcgatctg tgaccccgcg
     2641 gcggtgtacc gctccgacgc gtcggccatc gcctgcttcg acgcgtcgtc tgtgccggtg
     2701 aggttgagca cctgaccgcc gagccgctcg atcacgcggc gagcgtcggc tttcgcgtcg
     2761 gccagcgagg ctgcggtccg gttgcgtgat gcccgcgacg agctgaccac cgccagcgca
     2821 atcgccgcga cgaccagaat gaggatgact atcagcgccg cgttcatgcg accaccctac
     2881 cgacgggcgc ttgcgccgag aacgacgttt tgcagggctc ttctcgcact tcctctgcga
     2941 aacgtcgctt tcgacggaac ggagcgggcg acgggaatcg aacccgcgta gctagtttgg
     3001 aagactaggg ctctaccact gagctacgcc cgcagcacag cacgaaggca ctgtacggtg
     3061 ctttcgcgcg ccaagccaag cgggccgtag gatcgcctgg ttactggaat gtgtgcagta
     3121 cgcggggtgt agcgcagctt ggtagcgcat ccgctttggg agcggaaggc cgcaggttca
     3181 aatcctgtca ccccgaccag acgtgaccga gacagatgag gagcctgaac acgtgaagag
     3241 caccgttgag cagctgagcc ccacccgggt ccggatcaac gtcgaggtgc cgtttaccga
     3301 gctgcagccc gacttcgacc gcgcgtacaa gcagctggcc cagcaggtcc gcctgcccgg
     3361 gtttcgtccg ggcaaggcgc cggccaagct gctcgaagcc cggatcggcc gcggggcggt
     3421 gctggagcag gtggtcaacg acgcgctgcc gatccgctac agcgaggccg tcacgtcctc
     3481 ggatgtgcag ccgatcggtc agccggagat cgagatcacc aagatcgaag acggcgacga
     3541 gctggtgttc accgccgaag tcgacgtgcg cccgaaaatc gagctgccgg agctggacgc
     3601 tgtgacgatt gaagtcgacc cggtcgaagt gaccgacgag gacgtggacg ccgagttgga
     3661 gtcgttgcgc gcccgcttcg gcacgctgac cggagtcgaa cgggccgcgc agaccggaga
     3721 cttcgtgtcg atcgacctgt cggcgacggt cgacggccag gaggtgccgg aggcggcgac
     3781 cgaagggctg tcgcacgaag tcgggtcggg ccagctcgtc gagggtctcg acgacgcggt
     3841 cgtcgggttg tcggcagggg agagccgcgt gttcaccacc acgctggccg ccggcgagca
     3901 cgccggccag agcgcgcagg tgaccgtcac cgtgaactcg gtcaaggaac gcgagctgcc
     3961 ggagcccgac gacgaattcg cgcaactggc aagcgaattc gacacgatcg acgaactgag
     4021 ggccagtctc ggcgatcagg tggcgcggat caagcggatc caacaggccg agaaaatccg
     4081 ggatgccgcg atcgagacgc tgctcggcca ggtggaggtg ccgcttccgg aggcgatcgt
     4141 gcaggcgcag gtcgacgaga ccgtgcacaa cgctctgcac ggtctcgacc atgacgaaga
     4201 gcgactcgcc gaggcgttgg ccgagcaagg cagcagccgc gagcagttcg acgccgacgc
     4261 gcgcgagaac gcggagaagg cgatcaagac ccagctgctg gtcgatgcga tcgccgacaa
     4321 actcgaggtg caggtcggcc aggacgattt gaccgagcgg ctggtgctgc agtcgcggca
     4381 gtacggcgtc gagccgcagc agctgctgca gttccttcag gagaacaacc agctgccggc
     4441 gatgtacgcc gacgtgcgcc gcggcaaaac gctggcggcg gtggtgcgtg gtgcgacggt
     4501 cgccgacacg gccgggaaca ccgtcgacac cgacgagttc ttcggcccgc gcgaacaggc
     4561 cgaggctgag gccgacgaaa caacagacga cgaggaccag taacgctgtg agcgaacccg
     4621 gatgttgtcg ggagtgcgcg gcgcggcgag ttggttagtg tcaggtgaag caaccctaga
     4681 aagcaggtat tggctgtgac tgatatgcgt tccagcgcgc cggggctcaa cctcgtcgac
     4741 tcggtgtacg agcgactgct gaccgagcgc atcatcttcc tgggctctca ggtcgacgac
     4801 gacatcgcca accggttgtg cgcgcagata ctgctgctgg cggcggagga tcccaccaag
     4861 gacatccacc tgtacatcaa ctcgccgggt ggctcgatca gcgccggcat ggccatctac
     4921 gacacgatgg tgttggcccc ctgcgacgtc gcgacctacg cgatgggcat ggccgcgtca
     4981 atgggccaat ttctgctggc ggcaggcacc aagggcaagc gctacgcact gccgcacgcc
     5041 cgcatcctga tgcaccagcc gctgggcggc gtcactggca gcgcggcgga catcgcgatc
     5101 caggccgagc agttcgcggt gatcaagaag gagatgttcc ggctccacgc ggagttcccc
     5161 ggccagccgt tggagcgcat cgtcgcagac tccgaccgcg accggtggtt caccgcgccg
     5221 gaggcactgg agtacggctt
//
LOCUS       DASWBF010000464         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_518306,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000464 DASWBF010000000
VERSION     DASWBF010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518306"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1135
                     /locus_tag="VF874_17035"
     CDS             269..1135
                     /locus_tag="VF874_17035"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9177701.1"
                     /translation="MGIPEAAESAAETPTCYRHPGRESHVRCARCRRYICPQCMREAT
                     VGHQCVECVDAGARTVRRPRSRLGGKQRPGTPVITYALIGLNTLVFVIDAASARVERL
                     LTLWSPGVAHGELYRLVTSAFVHYGVMHLLFNMWALYVVGPPLEMWLGRLRFGALYAL
                     SALGGSVLVYLLSPLNASTAGASAAVFGLFGATFVVAKRLHLDVRWVVALIVLNLVFT
                     LAVPGISWQGHVGGLVTGALVALGYVYAPRQHRNLVQAAVTGAFVVLFAVLIWWRTTQ
                     LLAEFGRPLGLG"
     gene            complement(1410..2222)
                     /locus_tag="VF874_17040"
     CDS             complement(1410..2222)
                     /locus_tag="VF874_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006896726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9177702.1"
                     /translation="MTPVDRPIRVAAHLQLQHADYDTVRRAAREAENIGVDIVFNYDH
                     FFPLSGDPNGKVFECWTVLGAWAESTSRVGIGALVSCNAYRNPDLLADMARTVDHISG
                     GRLILGIGAGWWQKDFDEYGYTFGTAGQRLDGLAAAMPRIEHRLARLNPPPTRKIPVL
                     IGGGGEKKTLRIVAEHADIWHAFGDRDSLRHKVDVLEQHCRAVGRDVNDIERSGPIVP
                     SPAIPVDEAGDHATPDQLRELGFTLFTWTIGGPNYDLEPLREWIGWRDKHNG"
     gene            complement(2425..2724)
                     /locus_tag="VF874_17045"
     CDS             complement(2425..2724)
                     /locus_tag="VF874_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177703.1"
                     /translation="MPDEAVHEDTTTPDDGEPDTGRLFTIKDVAAASGLPQPAAAQVV
                     PRTWTPKGWMYTAAQVRYAAEVGQTMRSQRATPSPPAIKGAEPTDINQRARRQTE"
     gene            complement(2840..3023)
                     /locus_tag="VF874_17050"
                     /pseudo
     CDS             complement(2840..3023)
                     /locus_tag="VF874_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01777 family protein"
BASE COUNT          429 a          952 c         1048 g          594 t
ORIGIN      
        1 ggcgccggtt gaccgcagcg gcccggcggc cgccaggagc gggccggcag gtcgccaaac
       61 ggcactgtca acttgagcaa tccgagggcg attttcgccg gttcgcctcc gcgattcacc
      121 aggtcgcgtg tgacgtatga ggcttatgcg gttagcagaa cgtccacacg acctcaagtt
      181 gacagtgcca ttgcacccgt cgcccaccgg tgcccacgac gacgcgtcca cgccgccgcc
      241 tggtagccga ggtgatcaca ctggagggat gggcatcccc gaggctgcgg agtcagcggc
      301 cgagacgccg acgtgttacc ggcatcccgg ccgcgagtcg catgtccgct gcgcccgttg
      361 ccgccgctac atctgcccgc agtgcatgcg cgaggccacc gttgggcacc agtgcgtcga
      421 gtgtgtcgac gcgggcgcca ggacggtgcg acggccacgc tcgcgtttgg gcgggaagca
      481 gcggccgggc acgccggtca tcacgtacgc gctgatcggg ctcaatacgc tggtgttcgt
      541 catcgacgcc gcgtcggcac gcgtggaaag gctgctgacg ctgtggtcgc cgggcgtcgc
      601 ccacggcgag ctgtaccgct tggtgacctc tgcgttcgtg cactacgggg tgatgcacct
      661 gctgttcaac atgtgggcgc tctatgtggt gggcccgccg ttggagatgt ggctcggccg
      721 gttgcggttc ggcgcgctct acgcgctcag cgcgttgggc ggttcggtgc tggtgtatct
      781 gctctccccg ctgaacgctt ccaccgcggg tgcgtcggcc gctgtgttcg gcctgttcgg
      841 cgcgacgttt gtggtggcaa agcggctgca cctcgatgtc cgttgggtcg tcgcgctgat
      901 cgtactcaac ctggttttca ccctcgcggt tccggggatc agttggcagg gccacgtcgg
      961 cggacttgtc accggtgcgc tggtcgcgct cggttacgtg tacgcacccc ggcaacaccg
     1021 aaacctggtg caggcagcag tgacgggcgc cttcgtggtg ctgttcgcgg tgttgatctg
     1081 gtggcggaca acgcagcttc tcgcggagtt cggccggcct ctcggtcttg gttgagcgcg
     1141 tgctgcagcg gactgcgttt cgttgcggtc tcggccgcct tgtctgctgg cgggtcaccc
     1201 aagaacggcg tcggccatcg actgtccacc gcgcgcgtac gacgtgagct cacgcaccag
     1261 ccgcgccgat ctggctttgg tgtttgtgtc gtacctgccg gccatcgcgc catccttcct
     1321 cggcagaggg tgtgcacgaa acccgggata gttcacccga tgcttgccaa ccggttcttc
     1381 gaaaaccggt gcagcgcagg ggcgaggcat caaccgttat gtttgtcgcg ccagccgatc
     1441 cattcccgca ggggctcgag gtcgtagttg gggccaccga tggtccaggt gaataacgtg
     1501 aacccgagtt cgcgcagttg gtcgggtgtg gcgtggtcac ccgcctcgtc gactgggata
     1561 gcgggagacg gcacgatggg accggaacgt tcgatgtcgt ttacgtcgcg cccgaccgcc
     1621 cggcaatgct gttcgagcac atccaccttg tggcgcaggc tgtcccggtc gccgaaagcg
     1681 tgccagatgt cggcatgctc tgcgacgatc cgcagtgtct tcttttcgcc gcccccaccg
     1741 atgaggaccg gaatcttgcg ggtcggtggg gggttcagtc gggccagccg gtgctcgatt
     1801 cgcggcatcg ccgcggcgag gccgtcgagg cgttgccctg ccgtgccgaa cgtatagccg
     1861 tattcgtcga agtccttctg ccaccagccg gcgccgatgc ccaggatgag ccgtccaccg
     1921 ctgatgtgat cgacggtgcg ggccatgtcg gccagcaggt ccgggttgcg gtaggcgttg
     1981 cagctcacca gggcaccgat ccccacccgg ctggtggatt cggcccaggc gcccagcacc
     2041 gtccagcatt caaaaacctt gccgttcggg tcgccggaga gtgggaagaa gtggtcgtag
     2101 ttgaacacga tgtccacgcc gatgttttcg gcttcccggg cggcgcggcg gaccgtgtca
     2161 tagtcggcat gctgcagctg cagatgagcg gcaactcgga tgggtctgtc cactggcgtc
     2221 atgctcgaaa tcctttcctg atctcagcgc cggctcactt cccgccgaaa cgagggagcc
     2281 gtgacctcga agggagaggc gtccaggtac ggcatcgctt cgcttcagcg gcaatgatcc
     2341 cagttcttct cgccgctgcc ttcagcagcc gggtctttgg cggcacgggg tgaacgcgct
     2401 gggtggatgg ctaccgtccc gcgatcactc cgtttggcgg cgggcgcgtt gatttatgtc
     2461 ggtgggttcg gcacctttga tcgccggcgg tgaaggcgtg gctcgttgcg atcgcatcgt
     2521 ctggcccact tctgccgcgt agcgcacctg cgccgcggtg tacatccagc ctttcggagt
     2581 ccaggtacgc ggaaccacct gggccgcagc aggttgcggt agtccggacg ctgccgccac
     2641 gtccttgatg gtgaacagac gacccgtatc gggctccccg tcgtcgggtg ttgtggtgtc
     2701 ttcgtggacg gcttcgtcgg gcatacatgt agattacggc gaccatgacg gccggtcttc
     2761 gattgccggt gttgcagacg ccgtgcaatc tgggccggtg cgggttcgtc ggccaggatg
     2821 ggcatttcgt ctggcccggt gcgccctggt cgccgagcag cagccgcgga cccagcggag
     2881 ggaccggcaa caccgctggt cggcgcagga catgcgcgag cgtgcgagtg tagtcgacgt
     2941 tgcgaaccgg ttctggggca accgcattca ccggcccgga cagggcggtg tcccacagcc
     3001 cgcgatagta ggtgtcgacg agg
//
LOCUS       DASWBF010000465         5946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_781238,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000465 DASWBF010000000
VERSION     DASWBF010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781238"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VF874_17055"
     CDS             complement(<1..462)
                     /locus_tag="VF874_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX9177704.1"
                     /translation="MTDQGTGEELRGGHRDEAAAAGSGIDVVLVTGLSGAGRGTAAKV
                     LEDLGWYVADNLPPELIAQMVDLGLAAGSRITQLAVVLDVRSRGFTGDLEWVREDLAS
                     RGIHPRVLFLEASDDVLVRRYEHVRRSHPLQGDQTLAEGIAAERRMLAPLRE"
     gene            complement(459..2432)
                     /gene="uvrC"
                     /locus_tag="VF874_17060"
     CDS             complement(459..2432)
                     /gene="uvrC"
                     /locus_tag="VF874_17060"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX9177705.1"
                     /translation="MPDPSTYRPAPGSIPVEPGVYRFRDPHSRVIYVGKAKSLRSRLT
                     SYFADISSLPPRTRQLVTTAASVEWTVVTTEVEALQLEYNWIKEFDPRFNVRYRDDKS
                     YPVLAVTLNEEYPRLMVYRGPRRKGVRYFGPYSHAWAIRETLDLLTRVFPARTCSAGV
                     FKRHRQIDRPCLLGYIDKCSAPCINRVSADEHRKIVLDFCDFLSGKTDRLIRDMEQQM
                     NEAAATLDFERAARLRDNISALKRALEKQAVVFGDGTDADLVAFADDDLEAAVQVFHV
                     RGGRVRGQRGWIVEKSAGPGESGHQVLVEQFLTQFYGEQAELDGAADEFTNPVPRQVL
                     VPVLPENTDELAEWLSRLRGSRVSLRVPQRGDKRALAETVQRNAAEALAQHKLKRAGD
                     FNARSEALQSIQDALGLAEAPLRIECVDVSHVQGTDVVASLVVFEDALPKRSDYRHFA
                     IREAAGGGRSDDVASIAEVTRRRFLRHLSDAAQAEPRARRFAYPPNLFVVDGGAPQVN
                     AAQAVLDELAITDVAVIGLAKRLEEVWVPSEPDPVIMPRNSEGLYLLQRVRDEAHRFA
                     IAYHRSKRSKRMTASALDSVRGLGEARRKALVAHFGSVARLKQASVDEITAVPGIGVA
                     TARAVLEALGVTDSAGTSAEAAAMGEDRTRASG"
     gene            complement(2538..2978)
                     /locus_tag="VF874_17065"
     CDS             complement(2538..2978)
                     /locus_tag="VF874_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX9177706.1"
                     /translation="MTGDWELQVRPHLTPYFAYGAAILILAAHVAVGFFLKAGSTGVV
                     FQTADQVAIAGLGVVIACAMLLFARPRLRAGKDGLAVRNLLNEKLIAWPEVVDVSFPV
                     GARWARVDLPDDEYIPLMAIQAVDKERAVAAMDAVRELVTRYRP"
     gene            complement(2975..3400)
                     /gene="ribH"
                     /locus_tag="VF874_17070"
     CDS             complement(2975..3400)
                     /gene="ribH"
                     /locus_tag="VF874_17070"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9177707.1"
                     /translation="MRLAIVASTWHQKICDALLDSAIRVAKDAGVRKPTVARVLGAIE
                     IPVVAQELARNHEAVVALGVVIRGQTPHFDYVCDAVTQGLTRVSLDASTPVANGVLTT
                     DNEEQALDRAGLPSSAEDKGAQATAAALSTALTLRHLRS"
     gene            complement(3483..4739)
                     /locus_tag="VF874_17075"
     CDS             complement(3483..4739)
                     /locus_tag="VF874_17075"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX9177708.1"
                     /translation="MTRFDSVERAVAEIAAGNAVVVVDDEDRENEGDLIFAAEMATPE
                     LVAFMVRYTSGYLCVPLDGEICDRLGLLPMYAVNQDKHGTAYTVTVDARNGVGTGISA
                     SDRATTMRLLADPASIASDFTRPGHVVPLRAKEGGVLRRPGHTEAAVDLAKLAGLQPA
                     GAICEIVSQKDEGAMAQTEELRVFADEHNLALISIADLIEWRRKHEKHVRLVAEARIP
                     TRHGEFRAVGYTSIYDDVEHVALVRGDIGGGDDVLVRVHSECLTGDVFGSRRCDCGPQ
                     LDAAMAMVAREGRGVVLYMRGHEGRGIGLMHKLQAYQLQDAGADTVDANLELGLPADA
                     RDYGLGAQILYDLGVRSMRLLTNNPAKRVGLDGYGLHITERVPLPVRANAENIRYLIT
                     KRDRMGHDLSGLDDFDHLPGEFGGAL"
     gene            complement(5796..>5946)
                     /locus_tag="VF874_17080"
     CDS             complement(5796..>5946)
                     /locus_tag="VF874_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9177709.1"
                     /translation="AGQDWFEVSLIPTTLEETTLASVPVSGEVNLEVDVIAKYVERLM
                     QMGNR"
BASE COUNT          986 a         2072 c         1850 g         1038 t
ORIGIN      
        1 ttcgcgcagc ggcgccagca tcctgcgctc ggccgcgatt ccttcggcca gtgtctggtc
       61 gccctgcagc ggatggcttc gccgcacatg ctcgtagcgt cggacgagga cgtcgtcgga
      121 ggcctccaag aacagcaccc gcgggtgaat gccgcgcgac gccaaatcct cacgcaccca
      181 ttccaagtcg ccggtgaacc cgcgggagcg cacgtcgagc acgaccgcca gctgagtgat
      241 ccgcgacccg gcggccaacc cgaggtccac catctgggcg atcagctccg gtggcaggtt
      301 gtcggccacg taccagccca ggtcttcgag caccttggcg gcggtaccgc gccctgctcc
      361 ggacagcccg gtgaccagga cgacgtcgat gccggatccc gccgcagccg cctcgtcccg
      421 gtggccgccc cgcagctctt cgcccgtgcc ctggtctgtc atccggatgc cctggttcga
      481 tcttcaccca ttgcggcggc ctccgcgctg gttcccgccg aatcggtcac cccgagcgcc
      541 tcgagcacgg cccgcgccgt cgccaccccg attcccggca ccgcggtgat ctcgtccacc
      601 gacgcctgct tcagcctggc caccgacccg aagtgcgcga ccagcgcctt gcgccgcgcc
      661 tcgcccagcc cccgtaccga gtccagcgcc gaggcggtca tccgcttgga gcgcttgctg
      721 cggtgatagg cgatcgcgaa ccggtgcgcc tcgtcgcgga cgcgctgcag caggtacagg
      781 ccctcgctgt tgcggggcat gatcaccgga tcgggctccg acggcaccca cacctcttcg
      841 agccgcttgg ccaggccgat caccgcgacg tcggtgatgg cgagctcgtc gagcaccgcc
      901 tgcgcggcgt tcacctgagg cgcaccgccg tcgaccacga aaaggtttgg cgggtacgcg
      961 aacctgcgcg cccttggctc ggcctgcgca gcgtccgaca gatgccgcaa gaaccggcgc
     1021 cgcgtgacct ccgcgatcga cgcgacatcg tcggaccgtc cgccaccggc cgcttcgcgg
     1081 atcgcgaaat ggcggtagtc cgagcgcttc ggcaacgcgt cctcgaacac caccagcgac
     1141 gccaccacgt cggtgccctg cacatggctg acgtccacgc actcgatgcg cagcggcgct
     1201 tcagcgagcc cgagcgcatc ctgaatactc tgcagcgctt cggatctcgc gttgaagtcg
     1261 ccggctctct tcagcttgtg ctgggccagc gcctcggctg cgttgcgctg tacggtttcg
     1321 gccagcgccc gcttgtcgcc gcgctgcgga accctcagag acacccgcga gccacgcagc
     1381 cgggacagcc actccgcaag ttcgtcggtg ttttcgggca acaccggtac caacacctgc
     1441 cgcggtacgg ggttggtgaa ctcgtctgcg gcgccgtcca attcggcctg ctcgccgtag
     1501 aactgggtga ggaactgctc caccagcacc tggtggccgg attcgcccgg accagcggac
     1561 ttctcgacga tccacccgcg ctgaccgcgc acccggccgc cgcgaacgtg gaacacctgc
     1621 accgcggcct ccaggtcgtc gtcggcgaac gcgacgaggt cggcgtcggt gccgtcaccg
     1681 aacaccacgg cctgcttttc cagcgcgcgt ttgagcgcag agatgttgtc ccgcaaccgc
     1741 gccgcccgct cgaaatccag cgtggcggcg gcctcgttca tctgctgctc catgtcgcgg
     1801 atcagccgat cggtcttgcc ggacaggaag tcacagaaat cgagcacgat ctttcggtgc
     1861 tcgtcggcgc tcaccctgtt gatgcacggc gccgagcact tgtcgatgta tcccaacagg
     1921 catggccggt caatctgcct gtgccgctta aacactccag ccgagcatgt gcgggccggg
     1981 aacacccggg tgagcaagtc cagcgtctcg cgaatcgccc aggcgtgcga gtacgggccg
     2041 aagtagcgga cacccttgcg gcggggtccg cggtacacca tcagcctcgg gtattcctcg
     2101 ttgagcgtta ccgcgagcac cgggtaggac ttgtcgtcgc ggtagcgcac gttgaaccgc
     2161 ggatcgaact ccttgatcca gttgtactcg agttgcagcg cctcgacctc ggtggtgacg
     2221 acggtccact ccacgctcgc cgcggtggtc accaactgcc gtgttctcgg cggcaggctg
     2281 gagatgtcgg cgaaatagga cgtcaatctg ctgcgcaggc tcttggcctt gccgacgtag
     2341 ataacccggc tgtgcggatc gcggaagcga tagacacctg gctcgacggg aatcgacccg
     2401 ggagcaggcc ggtacgtcga ggggtcaggc acggtctcag gctagtacgg gcgccgcggg
     2461 agtctcccat gcgccgagat cgacgttttg cagaagaagc ccgagtagcg gcctgcaaaa
     2521 cgtcactttc gacgaagcta gggccggtag cgggtgacca gttcgcgcac cgcgtccatc
     2581 gcggccaccg cccgctcctt gtccaccgcc tggatcgcca tcagcgggat gtattcgtcg
     2641 tcgggcaggt cgacgcgcgc ccaccgggca ccgaccggaa acgacacgtc cactacctcg
     2701 ggccacgcaa tcagtttctc gttcaacagg tttcgcacgg ccagtccgtc cttgccggcc
     2761 cgcagccgcg gtcgcgcaaa cagcagcatc gcgcacgcga tcaccacgcc gagcccggcg
     2821 atcgcaacct gatccgccgt ttggaacacc acgccggtgg aaccggcctt cagaaaaaag
     2881 ccgacggcga catgcgccgc caagatcagg atcgctgcgc cgtacgcgaa atacggggtc
     2941 aagtgcggcc gtacctgcaa ctcccagtcg cctgtcatga ccgcaagtgc cgcagcgtca
     3001 gcgccgtgga caacgccgcg gccgtggcct gggctccttt gtcctccgcc gacgacggca
     3061 accctgcgcg gtccaacgcc tgctcctcgt tgtccgtggt cagcacgccg ttggccaccg
     3121 gtgtgctggc gtccagcgag acgcgcgtca gaccctgggt aaccgcatcg cagacgtagt
     3181 cgaaatgcgg tgtctggccg cggatcacca caccaagtgc gacgacggcc tcgtgattgc
     3241 gggccaattc ctgtgctacg accgggattt cgatcgcacc gagcacccgt gccaccgtgg
     3301 gcttccgtac gccggcgtct ttggcgacgc gtatcgcgct gtcgaggagg gcatcacaga
     3361 ttttctgatg ccacgtgctc gccacgatgg ccagccgcat acccgacgcg tcaagctccg
     3421 gcaagtccgg caccccgtgc gcaggactca cgcgcgatcc gccttcgctt ctcgctgacc
     3481 gctcaaaggg caccgccgaa ttcgcccggc aggtggtcga agtcgtcaag accagacagg
     3541 tcgtggccca tccggtcgcg cttggtgatc aggtaccgga tgttctcggc gttggcgcgc
     3601 accggcagcg gcacccgctc ggtgatgtgc agcccgtacc cgtcgaggcc gacccgcttg
     3661 gcggggttgt tggtgagcag ccgcatcgag cgcaccccga ggtcgtacag aatctgcgcg
     3721 cccaggccgt agtcgcgggc gtcggcgggc agccccagtt ccaggttggc gtccacggta
     3781 tcggcacccg cgtcctgcag ctggtaggcc tgcagcttgt gcatcagccc gatgccgcgg
     3841 ccctcgtggc cgcgcatgta gagcacgacg ccgcggcctt cgcgcgcgac catcgccatc
     3901 gccgcgtcga gctgcggacc gcagtcgcag cggcgtgagc cgaacacgtc gccggtgagg
     3961 cactcggagt gcacgcggac caatacgtcg tcgccgccgc cgatgtctcc gcgcaccagt
     4021 gcgacatgct cgacgtcgtc gtagatgctg gtatagccga cggcccggaa ctcgccgtgc
     4081 cgggtgggaa tgcgcgcctc ggcgaccagc ctgacgtgct tctcgtgctt gcgtcgccac
     4141 tcgatgaggt cggcaatcga gatcagcgcg aggttgtgct cgtcggcgaa cacccgcagt
     4201 tcctcggtct gcgccatcgc gccttcgtcc ttttggctga cgatctcgca gatcgcgccg
     4261 gcgggctgca gcccggccag cttcgccaaa tcgaccgcgg cttcggtgtg cccaggccgg
     4321 cgcaacacgc cgccctcctt ggcccgcaac ggcaccacgt gaccgggtcg ggtgaaatcg
     4381 cttgcgatgc ttgccgggtc ggccaacagc cgcatcgtcg tggcccggtc agaagccgaa
     4441 atgccggtcc ccacaccgtt tctcgcgtcg accgtcaccg tgtaggcggt gccgtgcttg
     4501 tcctgattca ccgcatacat cggcaaaagg ccgagtcggt cgcagatctc gccgtccaac
     4561 ggcacacaca ggtaaccgga ggtgtagcgc accatgaacg cgacgagctc gggcgtcgcc
     4621 atctcggcgg cgaaaatcag atcaccctcg ttctcgcggt cctcgtcgtc gacgacgacc
     4681 actgcgttgc ccgccgcgat ttcggcgacc gcgcgctcga cactgtcgaa cctggtcatc
     4741 ggccggtaac tccttcgggc ggcggcgcag tgcaaaggcg ctgctccccc gagtctagtg
     4801 accagcgcct ataagtattt tcgacgtact tcgcaagtag gggtacgcag gcggtgtgcc
     4861 aacgctgaga agggcaacac cgcaggtcag cggtgtgcgc cagaatatgt ttgtgtggcc
     4921 tggccggatg gatggggttt tgcggcctca acgtcaacgt gggcgagtca acacatgcgt
     4981 aactagcagg ggtcgccagg aggtgtgtag tacggcacac cttcaaccgt gtagcacggc
     5041 acctcgccag gaacgtatgg gtcggtggcc gacgctatgg ccaccccggt cgccacgtca
     5101 ccggctacgt tggtgcagcc acccaccccg acatggcgtc catgtgcgtc cgcgcaaatg
     5161 tcggcgtgcg ctaccggtgc tattgcgact ggcactatcg ccgtcgctgt ggttgtcaac
     5221 agcgctgctg ccagtttctt catcgcatac ttccttctgc tagtccggcc cggcccggcg
     5281 cttattatcg tccccgacta gtcccgggcc ctaagtatca accaaagaat tcacactcga
     5341 accgggcgag actcactgtt ggggtgttgc cactgctagc tgcgccgccg gtatcgggcg
     5401 gaagcggcga cggtgacggc caaccgcacc cgtcctggcc gcgtgtttcg tcccgagcac
     5461 attgacatca agttccggga cgccgagtag cctgggtcgc cgctttccac cttgccaaga
     5521 aggccgcagg cagtaatcgg aggggttatc gaaccttctc gaagtcgtcg cccgacgtgt
     5581 aatgaaacgg taagttcgtg cccgttatgt tttcaattag ggccgcgccc agcggcttaa
     5641 cctcgtagta gtagtgaatg ttgccctgtg catcaggtgc cgtaacacat aggcccttcg
     5701 ggcacttttc cttggtcggt agggcgagca cgccgatcac cagcaccacg gcaatcccga
     5761 ccagaaccag aagtagaact ctagttcatg tgtattcacc tgttgcccat ctgcataagt
     5821 cgctcaacat atttcgcaat cacgtcgacc tccaggttca cctcacccga tactggaaca
     5881 ctcgccagcg tggtttcctc gagcgtggtg gggatcagcg acacctcgaa ccagtcttga
     5941 cctgcg
//
LOCUS       DASWBF010000466         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_634459,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000466 DASWBF010000000
VERSION     DASWBF010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634459"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VF874_17085"
     CDS             <1..300
                     /locus_tag="VF874_17085"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX9177710.1"
                     /translation="AYVVPEKYARLGQLLFGGHDAEDSRERLFTEVDKLLHRLDMPHS
                     LKEYGIAEEEFVATLPELAMTAFEDLSNRTNPRMPLVSEITALLKLGYYGSAHSD"
     gene            complement(306..1778)
                     /gene="lnt"
                     /locus_tag="VF874_17090"
     CDS             complement(306..1778)
                     /gene="lnt"
                     /locus_tag="VF874_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9177711.1"
                     /translation="MNQRPRRAPGRAAAVVAGALPALAFPAPSWWWLAWVGVVPLLLV
                     VSAAPTAWEGGVQAWFGLGGFVLATQYWLLPSAGPLLVGMAAILGALWVPWGWAAQRL
                     LSAPATAGRTAAAVVVLPSTWVLAEAVRSWQSLGGPWALLGASQWSQPVSLASASLGG
                     VWLTSFLIAAANTAIAAAIMHRDMRGRVTAMAIALVCVGLGPAWFLLGPTPAPSRPAR
                     VALVQPGNVEDATARRQAGEALTQSLAGHHLDLVVWGESSVGVDLTSHPEVLQDLTDL
                     SRRLDAYLLINVDARAPAGGIYKSSVLIGPGGELGTYKKSRLVPFGEYVPLRQLLGPI
                     TRHTKAAAEDRRRGGGPVVLRAGSLAVAPLISYEATFSDMPRSAVQQGAELLAYQSST
                     STYQGTWAQPQLASQAAIHAVEVGRPAVHAALSGDSSAFDFRGRRLGLLSPDYRGAMV
                     VDVPLVSGTTMFQRLGDWVLALSISIVAGAGIGVALRYRR"
     gene            complement(1791..2292)
                     /locus_tag="VF874_17095"
                     /pseudo
     CDS             complement(1791..2292)
                     /locus_tag="VF874_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
     gene            2359..>2615
                     /locus_tag="VF874_17100"
     CDS             2359..>2615
                     /locus_tag="VF874_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177712.1"
                     /translation="MVARLSAFDPVVRVLVRVRLTLAALDVILVIVFRGKWRPGIDAV
                     RQFNKAVLNPAMLRMAGSKHWYASVVHHLGRMSGKPYSTPVV"
BASE COUNT          456 a          886 c          871 g          402 t
ORIGIN      
        1 gcgtacgtcg ttccggagaa gtacgcacga ctcgggcagc tgctcttcgg gggtcacgac
       61 gccgaggaca gtcgggagcg gctgtttact gaagtggaca agctgttgca tcggctcgac
      121 atgccgcact cgctgaagga atacggcatc gccgaggagg agttcgttgc gacgctgccg
      181 gaactggcga tgaccgcgtt cgaggatctg agcaaccgga ccaacccgcg aatgccgttg
      241 gtcagcgaga tcaccgcgct gctgaagctc ggctactacg ggtcggcgca ctcggattga
      301 cagcgtcagc gacggtatcg cagagctact ccgataccgg cgcccgcgac gattgatatc
      361 gacagcgcca gcacccaatc accgagccgc tgaaacatgg tggtgccgga gacgagcgga
      421 acatcgacca ccatcgcgcc gcgatagtcg ggcgaaagca agccgagtct gcgtccgcgg
      481 aagtcgaaag ctgagctgtc gccggacaat gccgcgtgca cggcagggcg gccgacttcg
      541 acagcgtgga tggccgcctg gctggcgagt tgcggctgcg cccaggtgcc ctgatatgtc
      601 gacgtggaac tctggtaggc cagcaactcg gcgccctgtt gcactgcact gcggggcatg
      661 tcggagaacg tcgcctcgta gctgatcagc ggtgcgacgg cgagcgaacc ggcccgcagc
      721 actaccggtc cgccaccgcg gcggcgatcc tcggccgcgg ccttggtgtg acgggtgatc
      781 gggccgagca actgccgcag gggcacatac tcgccgaacg gcaccagtcg gctcttcttg
      841 tacgtgccca gctctccgcc cggtccgatg aggacagacg acttgtagat cccccctgcc
      901 ggagcccgcg cgtcgacgtt gatgagcagg tacgcgtcga gccgacggga caggtctgtg
      961 aggtcttgca gcacctccgg atgacttgtg aggtcgacgc cgacactgct ctcaccccag
     1021 acgacgaggt cgaggtggtg accggcgagt gactgggtga gtgcttcgcc cgcctggcgg
     1081 cgcgccgtgg cgtcctcgac gttgccgggt tgcaccagcg ccacccgtgc cggtcggctc
     1141 ggcgcaggtg ttggtcccag caggaaccac gccgggccga gaccgacgca caccaatgcg
     1201 atcgccatag cggtgacgcg gccccgcata tcccggtgca tgatcgccgc ggcgatcgcg
     1261 gtgttggccg ccgcgatgag gaaactcgtc agccacaccc cgccgagcga ggcagaggcc
     1321 aagctgaccg gttggctcca ctgcgaggca cccagcaaag cccaagggcc gccaaggctt
     1381 tgccaagagc gcacggcttc ggccagcacc cacgtgctgg gcaatacgac cactgcggca
     1441 gctgtccgtc cggcggtggc aggcgccgac agcagccgct gtgcggccca gccccacggc
     1501 acccacaacg ccccgagtat cgcggccatc ccgaccagca gcggtccagc gctgggcagc
     1561 agccagtact gcgtggccag cacgaaaccg ccgaggccga accacgcctg cacgccgccc
     1621 tcccacgccg tcggcgcggc gctcaccacc agcaggagtg ggaccacgcc gacccaggcc
     1681 agccaccacc aggacggcgc gggaaaggcc agcgctggca gcgcaccggc aactacggcc
     1741 gcagcgcggc ctggcgcacg ccgtggacgc tggttcacag atcgcgcctg ttaccaaccc
     1801 acggaaccgg ggacgccctt gaacgggccg acgacccagc tggtgatcca gcctccgtag
     1861 tagccacccg gctgagggac gacctgtccg ccgttgatgg tgcagcggtc gaccaaaccg
     1921 ggcatgacag cgatcgcgcc ggcgagcggc cggtagccgg gtgtcggatt cagatatgtc
     1981 cacgcggcct gggacgcgac tctgtgttcg gtggccaggt cgaaatagct tgcctggcct
     2041 ttccattcgc accacgacga gcccggcgct tctcgtagta cccccggcga aaaagccctc
     2101 gcgcggaagg taataggtcg gcgggtggct ggtttccagc acccgccatg ccttttcggt
     2161 cgagccaatg gtcttgccgc ccagctcaac cgtgatcggc ccggtgaacg gctccagccg
     2221 cggcggtcgg gggtagtccc acgccgactc ctgaccggcg gcggctttgt cgggcttcgg
     2281 ccggacactc acagcggcct ccctctcacc agacggaagt tgaatcgtgc ctcggcgcac
     2341 cgtacttaag atgacggcat ggttgccagg ctgtcggcct tcgatccggt cgtccgtgtt
     2401 ctggtccgag tgcggttgac acttgcagcg ctcgacgtga tcctcgtcat cgtattccgc
     2461 gggaagtggc gcccggggat cgacgccgtc cgccagttca ataaggccgt cctcaacccg
     2521 gcgatgttgc ggatggcggg ctcgaaacac tggtacgcct ctgtggtcca ccaccttggc
     2581 aggatgtcgg gaaagcctta ctcgacgccc gttgt
//
LOCUS       DASWBF010000467         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1031493,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000467 DASWBF010000000
VERSION     DASWBF010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031493"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1411
                     /gene="hflX"
                     /locus_tag="VF874_17105"
     CDS             11..1411
                     /gene="hflX"
                     /locus_tag="VF874_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9177713.1"
                     /translation="MTYPEIPSSGELALEDRSALRRVSGLSTELADVSEVEYRQLRLE
                     RVVLVGVWTEGSAADTEASLAELAALAETAGSEVLEGLIQRRDKPDPSTYIGSGKARE
                     LREIVVATGADTVICDGELSPAQLTALEKAVKVKVIDRTALILDIFAQHATSREGKAQ
                     VSLAQMEYMLPRLRGWGESMSRQAGGRAGGSGGGVGLRGPGETKIETDRRRIRERIAK
                     LRREIKDMKQARDTRRGRRLRSDVPSIAIVGYTNAGKSSLLNALTGAGVLVQDALFAT
                     LEPTTRRGHFDDGRPFVLTDTVGFVRHLPTQLVEAFRSTLEEVVDADLLVHVVDGSDV
                     NPLAQIDAVRTVLREVVSDHDGTPAPELLVVNKCDATSDLAMAKLRRALPGAVFVSAH
                     TGEGLDELRRRMAELAVPTDTAIDVVIPYDRGDLVARVHADGRVQDSEHNAHGTRIRA
                     RVPLALAARLRDFATF"
     gene            complement(1408..>1696)
                     /locus_tag="VF874_17110"
     CDS             complement(1408..>1696)
                     /locus_tag="VF874_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177714.1"
                     /translation="GRLRESFVFEVRIADRSVTLLLRDGFVTEEFIDLAQKDDRTDED
                     ESRLDELKAQLAQRVMAADAADVYDIALGAETAYWQRNIEYGPAEIQFRRN"
BASE COUNT          289 a          548 c          583 g          276 t
ORIGIN      
        1 tttattgcct atgacatatc cagaaatccc cagttccggc gaactcgcgc tcgaagaccg
       61 gtccgcgctg cggcgcgtgt ccgggctgtc gaccgagctt gccgacgtct ccgaggtcga
      121 gtaccggcag ctgcggctgg aacgagtcgt gctcgtcggc gtatggaccg aaggcagcgc
      181 cgccgacacg gaggccagcc tcgccgagtt ggctgcgctg gccgagaccg ccggatcgga
      241 agtgctcgaa gggctgatcc agcgccgcga caagccagac ccgtcgacgt acatcggctc
      301 cggcaaggcg cgcgagttgc gggagatcgt cgtcgccacc ggcgccgaca ccgtgatctg
      361 cgacggcgag ctgagcccgg cacagctcac cgcgctggaa aaagcggtga aggtcaaagt
      421 gattgaccgc accgcgctga tcctcgacat tttcgcccag cacgccacca gccgcgaagg
      481 caaggcacag gtgtcgctgg ctcagatgga gtacatgctg ccgcggctgc gcggctgggg
      541 cgagtcgatg tcgcggcagg ccggcggacg cgcgggcggc agcggcggcg gcgtgggttt
      601 gcgaggcccc ggtgaaacca agatcgaaac cgaccgacgc cgcatccgcg agcgaatagc
      661 caagctgcgc cgcgagatca aggacatgaa gcaggcccgc gacactcggc gcggccgcag
      721 actgcgcagc gacgtgccgt cgatcgcgat cgtcggatac accaacgccg gcaaatccag
      781 tctgctcaac gcgctgaccg gcgcgggagt gctggtgcag gatgcgttgt tcgcgaccct
      841 cgaaccgacc acgcgccgtg ggcatttcga cgacgggcgc ccgttcgttc tgacggacac
      901 cgtcgggttc gtacggcatc tgccgacgca actcgtcgag gcgttccgct cgacgctgga
      961 ggaggtcgtc gatgccgatc tgctggtgca cgtcgtcgac ggctcggatg tgaacccgtt
     1021 ggcgcagatc gacgcggtgc ggaccgttct tcgcgaggtg gtttcagatc acgatggcac
     1081 cccggcgccg gagcttcttg tggtcaacaa gtgcgacgcg acaagcgatt tggcgatggc
     1141 gaagctgcgc agggcgctgc cgggagcggt gttcgtgtcg gcgcatacgg gcgagggcct
     1201 ggacgagctg cgcaggcgga tggccgaact ggccgtgccc accgacaccg cgatcgacgt
     1261 cgtcatcccg tatgaccgtg gcgatctggt ggcgcgcgtg cacgccgacg ggcgcgtgca
     1321 ggactccgag cacaacgcac acggcacccg aatcagggcg cgtgttccgc tggcgctggc
     1381 cgccaggctg cgcgatttcg cgaccttcta gttgcgccga aactgtattt cggcaggccc
     1441 gtactcgatg tttcgctgcc aatatgcagt ctcggcgcct agcgcgatgt cgtacacgtc
     1501 agccgcgtcg gcggccatca cccgttgcgc cagttgcgct ttcagctcgt cgaggcgcga
     1561 ctcatcttcg tcggtgcggt cgtctttctg cgcgaggtcg atgaactcct cggtgacgaa
     1621 gccgtcgcgc agcagcaggg taactgaacg atcggcgatc ctgacttcga acacaaagct
     1681 ttcccgcagc cggccg
//
LOCUS       DASWBF010000468         8087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_707783,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000468 DASWBF010000000
VERSION     DASWBF010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707783"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..707
                     /locus_tag="VF874_17115"
     CDS             222..707
                     /locus_tag="VF874_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9177715.1"
                     /translation="MTTTGPASRAELEERISADIRALSAESDQIGRLFASRHDVSPND
                     FRALLHVMVADTAGSPLTSGELRDRMGVSGAAITYLVERMIASDHLRRESDPADRRKV
                     LLRYADHGMAVARAFFAPLAEHTHVAMADLPDADLAAAHRVFSALVAAMRAFEAELNR
                     A"
     gene            complement(694..2292)
                     /locus_tag="VF874_17120"
     CDS             complement(694..2292)
                     /locus_tag="VF874_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX9177716.1"
                     /translation="MNTSLLLVLVAAMLLAAVARHYNVSAPLVLVVAGLAIGLIPGAP
                     TVQLDPDLVLFVLLPPLLWSAGLESSYVALRRNVRPIGLLAVGLPLATAFTVGLVAYH
                     TVPELTPGAALTLGAIVAPPDAIAALAVGRRLGLPRPMMTLLGGESLLNDATALTVYK
                     VALAAAIGTAIGWTQGLGTFALAAAGGTVIGLLFGVVVRLIRSRLDDPLMESGIGLVA
                     PFIVYLVAEEIHSSGVLAVVVAALFLGQRSTRASYATRLQDQAVWKALQLILESFAFL
                     LIGLQLPGVVRAMHGISPVSVVWSSVAVFLTVLLVRIVWIYVFTYVPRALFAGRRQGD
                     PKPPPAEVFVVAWAGMRGVVSLAAAFAVPLTTLSGAPFPGRPHIVFLTFVVVIGTLLL
                     HGLTLPWMIRVFGVQSDDAHRDAVALAAAQDRAARAAESRLEELVAEQQQSGRADIHE
                     HAAKILHGWNERRRNAAWERLGRSDEEIGEGPASAFRRLRLEMVAAERKTFVEERDLG
                     HIDDEVLRTALRGLDLEEATLRRD"
     gene            2312..2605
                     /locus_tag="VF874_17125"
     CDS             2312..2605
                     /locus_tag="VF874_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX9177717.1"
                     /translation="MLRRAPKPPDTCEHLTAAVGAQEPDPVTPGQCQDCAELGESRWA
                     HLRLCLSCGHVGCCDSSFHKHATAHFHATGHPVMRSAEPGETWRWCYVDVLLG"
     gene            2624..3862
                     /gene="purT"
                     /locus_tag="VF874_17130"
     CDS             2624..3862
                     /gene="purT"
                     /locus_tag="VF874_17130"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEX9177718.1"
                     /translation="MTEEPRPLSVMMLGAGELSRELVTAFQRLGVVVIAVDRYADAPA
                     HKVADHSVVIKMTDPDELTSLIEREQPDYVVTEARDPGSAPVIDYDALVAVGERGLTD
                     VVPTARSSQLSLDREGLRRLAADELGLPTAPFWFAASADELAAIARHAGFPLVVRPVA
                     GSASDGGSVLLRLEDVAAAWDRAMSAGGRVAHTRVMAETMVEIDHEVTLLTVRSAGRG
                     GPRVQFCEPIGHRQTDDEVLESWQPQEMTDAARDTAKSIAARIVNALGGRGVYGVELL
                     VKSNEVYFVDVTPRPYDSGLVTLRSQRLSQFELHARAILGLPVDTIMITPGAAKVVYA
                     SQGTGPANADLQHDDVRTERATQALSDALAVPESDVRIFGHPKSYPRRRLGVALATAA
                     NVNTARDRAHEAAAALRKFW"
     gene            3897..4310
                     /locus_tag="VF874_17135"
     CDS             3897..4310
                     /locus_tag="VF874_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9177719.1"
                     /translation="MSYAGDITPEEAWKLLSDNPAAVLVDCRTDAEWRFVGVADISDL
                     GRDVVYVQWSSVDGKRNEKFVDELIEAGVEPGERPVVFLCRSGNRSIAAAEAATEAGI
                     APSYNVLDGFEGNLDDSGHRGGSGWRAVGLPWRQS"
     gene            4318..5517
                     /locus_tag="VF874_17140"
     CDS             4318..5517
                     /locus_tag="VF874_17140"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX9177720.1"
                     /translation="MSIRTPAELPDGVSQATIGVRGALMRSGFEETAEAMYLTSGYVY
                     SNAAEAEKAFAGDIERYVYSRYGNPTISMFEERLRLIEGAPACFATATGMAAVFTSLG
                     ALLGAGDRLVSARSLFGSCFVVCDEILPRWGVETVFVDGDDLSQWEKALSEPTQAVFF
                     ETPSNPMQSLVDISAVCEMSHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHID
                     GQGRVLGGAILGDKEYIDGPVQKLMRHTGPAISAFNAWLLLKGLETLAVRVDYSTASA
                     LRIAEFLEEHPSVRWVRYPFLESHPQYDLAKRQMTGGGTVVTFELEGGNKQRAFEVLD
                     KLRLIDISNNLGDAKSLITHPATTTHRAMGPEGRAAIGLGDGVVRISVGLEGTDDLIN
                     DLDQALG"
     gene            5567..6106
                     /locus_tag="VF874_17145"
     CDS             5567..6106
                     /locus_tag="VF874_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177721.1"
                     /translation="MSKPTDRKKSRRQKRLARRAEWDTIVKLAEELADDAALNVAPRN
                     VTDVLLGVGNTPDDDYPADVVEVVSQAQQLERRILQRGWTFDSEFSSTAFASWFFEPS
                     AREFEDEALEQVTRVWITVNWDDVTDPNDFPYCVNVLFVGSGERDISRRPTLDDFFQQ
                     IDAIESYRVGDAAPIFDES"
     gene            complement(6229..7080)
                     /locus_tag="VF874_17150"
     CDS             complement(6229..7080)
                     /locus_tag="VF874_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177722.1"
                     /translation="MGNINGKTIVITGAARGIGHATANALLRLGARVVIGDRDVNALE
                     SAVTALDRMGPVSGYPLDVTNRQSFSEFLDKARSEAGGRIDVLINNAGVMPIGPFLQQ
                     SEQALRSSIEVNFYGVLNGCQLVLPEMVARRGGHIVNIASLAGMIAVPGQAVYAGTKF
                     AVVGLSVALADEFASQGVEVSAILPTFTNTELITGTHPSAAQKPVQPEQIAAAVVKVL
                     DKPTTVVSVPRYGRFMAATAQMLGPRGRRWLNKKLGNDRVFLDFDPTARQHYEDRAQH
                     ALGVTDD"
     gene            7143..7331
                     /locus_tag="VF874_17155"
                     /pseudo
     CDS             7143..7331
                     /locus_tag="VF874_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent monooxygenase"
     gene            7361..7507
                     /locus_tag="VF874_17160"
     CDS             7361..7507
                     /locus_tag="VF874_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177723.1"
                     /translation="MIDGQDYSKRRKTRSKWRADELVVTSVTHLHADRLRSHELIAER
                     WGIT"
     gene            7507..7964
                     /locus_tag="VF874_17165"
                     /pseudo
     CDS             7507..7964
                     /locus_tag="VF874_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246028.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1387 a         2669 c         2761 g         1270 t
ORIGIN      
        1 cggcttcggc gctggtgggc aggaactcgg gagactgagt gccggcctcg ttggcgccgg
       61 cgaggccgat caacgcgccg ccgagcaacg cgacggcaat cgcgatcagc cacgagcgac
      121 gagcggtgac caccgtggca aagcgatccc agaacaccgt cagataattt cagatactta
      181 acagttaatc aaatgaactt gcgcccgata tgctgctgtc gatgacgacg acgggcccgg
      241 cctcccgcgc ggaactggaa gagcggatct ccgccgacat ccgcgcgctg tccgcggagt
      301 ccgaccagat cggtcgcctg ttcgcatcgc gacacgacgt gagccccaac gactttcgcg
      361 ctctgctgca cgtgatggtc gccgacaccg cgggcagtcc actcacgtcg ggcgaactca
      421 gagaccggat gggcgtctcc ggtgcggcga tcacctacct cgtcgagcgg atgatcgcgt
      481 cggaccacct ccgccgcgag tccgatcccg ctgatcggcg caaggtgcta ctgaggtacg
      541 ccgaccacgg gatggccgtc gcaagagcgt ttttcgcgcc gttggccgag cacacgcacg
      601 tcgcgatggc cgaccttccc gacgccgact tggctgccgc gcaccgcgtc ttctccgcgc
      661 tcgtcgcggc gatgcgtgcg tttgaagcgg agctcaatcg cgcctgagcg tggcctcctc
      721 caggtccagt ccgcgcaacg cggtgcgcag cacctcatcg tcgatatgcc ccaggtcgcg
      781 ttcctcgacg aacgtcttcc gttcggcggc aaccatttcc agccgcagcc ggcggaacgc
      841 ggacgctggg ccttcgccga tctcttcgtc gcttcggccc agcctctccc acgcggcgtt
      901 gcggcgtcgc tcgttccagc cgtgcaggat ctttgcggcg tgctcgtgga tgtcggcgcg
      961 gcctgattgc tgctgttcgg ccactagctc ctcgagccgg ctttcggccg cacgggcggc
     1021 cctgtcctga gccgctgcca gcgcgaccgc gtcccggtgc gcgtcgtcgc tctgcacccc
     1081 gaagacccgg atcatccagg gcagcgtcag gccgtgcagc agcagcgtgc cgatcaccac
     1141 cacgaacgtc aggaacacga tgtgcggccg acccggaaac ggtgcgccgg acagcgtcgt
     1201 cagcggcacc gcgaacgccg cggccagtga cacgaccccg cgcattcccg cccaggccac
     1261 cacgaagacc tcggccggcg ggggcttggg gtcgccttgt cgccggccgg cgaacagcgc
     1321 ccgcggcacg tacgtgaaca cgtaaatcca gacgatccgc accagcagca ccgtcaggaa
     1381 caccgcgacc gacgaccaga cgacgctcac cggcgaaatg ccatgcatcg cgcgcaccac
     1441 gccgggcagc tgcaggccga tcagcagaaa cgcgaacgac tccaggatca gctgcagcgc
     1501 cttccagacg gcctggtcct gcagccgcgt cgcgtaactg gcgcgggtgg agcgttgccc
     1561 caaaaacagc gcggcgacga cgacggccag cacgccggag ctgtgaatct cctcggcgac
     1621 caggtacacg atgaacggcg cgaccagccc gatcccgctc tccatcagcg ggtcgtcgag
     1681 acgcgaccgg atcaacctga cgaccacgcc gaacagaagc ccgatcaccg tcccgccggc
     1741 ggccgccaac gcgaacgtgc ccaagccttg agtccagccg atcgcggtgc cgatcgcggc
     1801 ggccagcgcg accttgtaga cggtcagcgc ggtggcatcg ttgagcaggc tttcgccgcc
     1861 gagcagcgtc atcatcgggc ggggcaggcc caggcggcgg cccaccgcca gcgcggcgat
     1921 ggcgtcgggc ggtgccacga tcgcgcccag cgtcaacgcc gcgcccggcg tcagctcggg
     1981 cacggtgtgg tacgcgacga gcccgaccgt gaacgccgtc gccagcggca gtccgacggc
     2041 cagcagcccg atcggccgga cgttcctgcg tagcgccaca tacgagctct ccaacccggc
     2101 cgaccacagc aacggcggca ggagcacgaa cagcacgagg tccgggtcca gctggacggt
     2161 tggcgccccg gggatcagcc cgatcgccag gccggccacc accagcacca ggggcgccga
     2221 cacgttgtaa tgccgggcca ccgccgccaa cagcatcgcc gcgacgagga ccagcaacag
     2281 cgatgtgttc acgcccctat ccttgggcag catgcttcga cgcgcaccca agcccccgga
     2341 cacctgcgag cacctgaccg ctgctgtggg cgcccaggag cccgatcccg tcacacccgg
     2401 ccagtgccag gactgcgccg aactcggcga gtcgaggtgg gcccatttgc gactgtgctt
     2461 gagctgcggg cacgtcggct gctgcgactc cagctttcac aagcatgcga ctgcccactt
     2521 ccacgcgacg ggtcatccgg tgatgcgctc ggccgagccg ggcgaaacgt ggcggtggtg
     2581 ctacgtcgac gtgctgctcg ggtgagtctg gcaggctggc gacgtgaccg aagaaccacg
     2641 gccgctgagt gtgatgatgc tcggggcagg cgagttgagc cgcgagctgg tgacggcgtt
     2701 ccaacggctc ggtgtggtgg tgattgcggt cgaccgctac gcggacgcgc ccgcccacaa
     2761 ggtggccgac cacagcgtcg tcatcaagat gaccgatccc gacgagctga cctcgctgat
     2821 cgaacgtgag cagcccgatt acgtcgtcac cgaggcgaga gaccccggca gcgcgcccgt
     2881 catcgactac gatgcgctcg tcgccgtcgg tgagcggggg ctcacggacg tggttcccac
     2941 cgcgcgcagc agccagttga gcctggaccg tgagggcctg cggcggctgg ccgccgacga
     3001 gctggggttg ccgaccgcgc cgttctggtt cgccgcgtcg gccgacgaac tcgccgcgat
     3061 cgcgcggcac gccggctttc cgctggtggt gcggccggtc gcgggaagcg cgagtgacgg
     3121 cgggtccgtg ctgttgcgcc tggaagatgt cgcggcggcg tgggatcgcg cgatgagcgc
     3181 gggtggacgg gtggcgcata ctcgggtgat ggccgagacg atggtcgaga tcgaccacga
     3241 ggtcacgctg ctgaccgtgc gcagcgcagg gcggggagga ccgcgtgtgc agttctgcga
     3301 gccgatcggc caccggcaga ccgacgacga ggtgctcgag tcgtggcagc cgcaggagat
     3361 gaccgacgcg gcacgggaca cggcgaagtc gatcgcggcg cggatcgtca acgcgctcgg
     3421 aggccgcggg gtgtacggcg tcgagctgct ggtgaagagc aacgaggtgt acttcgtcga
     3481 cgtcactccg cgcccgtacg acagcggcct ggtcacactg cgatcgcagc ggttgtcgca
     3541 gttcgaactg catgcccgcg cgatcctcgg cctgccggtg gacacgatca tgatcacgcc
     3601 cggcgcggcg aaggtggtgt acgcgagcca gggaacgggg ccggcgaacg ccgatctgca
     3661 gcacgacgac gtgcggaccg agcgggcgac gcaggcgctt tcggacgcgc tcgcggtacc
     3721 ggagagcgac gtgcggatct tcgggcaccc caagagctac ccgcgacgcc ggctcggagt
     3781 ggcgctggcc accgccgcca acgtgaacac cgcccgcgat cgtgctcatg aagccgccgc
     3841 cgcgttgcgg aagttctggt aggcctaccg gtgaactggt atgggagact tccgaggtga
     3901 gctacgcagg tgacatcacg cccgaagagg catggaaact gctgagcgac aaccccgccg
     3961 ccgtgctggt cgactgccgc accgacgccg aatggcggtt cgtcggggtg gccgacatct
     4021 cggatctggg tcgcgacgtg gtgtacgtgc agtggtcgag cgtggacggc aagcgcaacg
     4081 agaagttcgt cgacgaactg atcgaggccg gcgtcgagcc cggcgagcgg cccgtcgtgt
     4141 tcctgtgtcg ctcgggcaac cggtcgatcg ccgccgccga ggccgccacc gaggcgggaa
     4201 ttgcgccgtc ctacaacgtg ttagacggct tcgagggcaa cctcgacgat tccggccatc
     4261 gtggcgggtc ggggtggcgc gccgtcggtc tgccctggag acagtcgtga agcagtcatg
     4321 agcattcgta cccccgccga actccccgac ggcgtcagcc aggccacgat cggtgtgcgc
     4381 ggcgcgttga tgcggtcggg cttcgaggaa accgccgagg cgatgtacct gacgtcgggc
     4441 tacgtctact cgaacgcggc ggaggcggag aaggcgttcg ccggcgacat cgagcgctac
     4501 gtgtattcgc ggtacggcaa cccgaccatc tcgatgttcg aggagcggct gcggctgatc
     4561 gagggagcac cggcgtgctt cgcgacggcg acgggaatgg ccgcggtgtt cacgtcgctg
     4621 ggggcactgt tgggcgcggg cgaccggctg gtgtccgcgc gcagcctgtt cggatcctgc
     4681 ttcgtggtgt gcgacgagat cctgccgcgc tggggcgtgg agacggtgtt cgtcgacggc
     4741 gacgacctgt cgcagtggga gaaggcgctg tcggagccga cgcaggcggt gttcttcgag
     4801 acgccgtcca acccgatgca gtcgctggtc gacatttcgg cggtgtgcga gatgtcgcac
     4861 gccgcgggcg caaaagtggt gctggacaac gtctttgcga caccgctgct gcagcagggc
     4921 tttcccttgg gcgtcgacgt cgtcgtctac tcggggacca agcacatcga cgggcagggc
     4981 cgggtgctgg gcggggcgat cctcggcgac aaggagtaca tcgacggccc ggtgcagaag
     5041 ctgatgcgcc acaccggccc ggcgatttcg gcgttcaacg cgtggctgtt gctcaagggg
     5101 ctggagacgt tggcggtgcg ggtggactac agcaccgcgt ccgctttacg gatcgcggag
     5161 ttcctggagg agcatccttc ggtgcggtgg gtgcgctacc cgttcttgga gtcgcacccg
     5221 cagtacgacc tggccaagcg gcagatgacc ggcggcggaa ccgtcgtcac cttcgaactc
     5281 gagggcggta acaagcagcg cgcgttcgag gtgctggaca agctgcggct gatcgacatc
     5341 agcaacaacc tcggtgatgc gaagtcgctg atcacccacc cggcgacgac gacccaccgc
     5401 gcgatggggc cggagggccg ggcggcgatc ggcctcggcg acggggtggt gcggatctcc
     5461 gtcggcctgg agggcaccga cgatttgatc aacgacctgg accaggcgct gggctaggcc
     5521 gcgtggcgtc gctcgtcgcg tcggccggcc gtcatagcat cgcgtcatgt cgaagccaac
     5581 cgaccgcaag aagtcccgcc gtcagaaacg gctggcgcgg cgcgcggaat gggacacgat
     5641 cgtgaagctc gccgaagagc ttgccgacga tgctgcgctg aacgtggcgc cccgcaacgt
     5701 caccgacgtt ctgctcggcg tcggcaatac gccggatgac gactatccag ccgacgtggt
     5761 ggaggtggtc agccaggccc aacaactcga acgccgaatc ctgcagcggg gctggacatt
     5821 cgactccgaa ttctccagca cggctttcgc cagctggttt ttcgagcctt cagcgagaga
     5881 attcgaggac gaagcactgg agcaggtgac ccgggtgtgg atcacagtga actgggacga
     5941 cgtcaccgac cccaacgact ttccatattg tgtcaatgtg cttttcgtcg gctccggcga
     6001 gagggacatc tcacgacgtc cgacgctgga cgacttcttc cagcagatcg atgcgatcga
     6061 gtcgtaccgc gtaggtgacg cggcgccgat attcgacgag tcgtgaaacg cacgctttga
     6121 agagaaaagt tgtcgcggaa ggcaatagtc gatctctccg ccttacagcg acaaagtgcg
     6181 agagggagcc tgcgaaccgt cagtttcgac gcggaaagct gcgcgcggct agtcgtcggt
     6241 gacgccgagc gcgtgttgcg cacggtcctc atagtgctgc cgcgcggtgg ggtcgaagtc
     6301 caggaacact cggtcgttgc ccagcttctt gttcagccag cgacgtccgc gcggacccag
     6361 catctgtgcc gtcgcggcca tgaaccgtcc ataccgcggc accgacacga ccgtcgttgg
     6421 tttgtcgagc actttgacca ctgcggcagc gatctgctcg ggctgcaccg gcttctgcgc
     6481 ggcggacggg tgcgtgccgg tgatcagttc ggtgttggtg aacgtcggca ggatcgcgct
     6541 gacttctact ccctgtgatg cgaactcgtc ggccaacgcc acggacagcc cgacgaccgc
     6601 aaatttcgtg cccgcgtaca ccgcttggcc gggcaccgcg atcatgccgg ccagcgacgc
     6661 gatgttgacg atgtgcccgc cgcggcgcgc caccatttcc ggcagcacca actggcagcc
     6721 gttgagcacg ccgtagaagt tcacctcgat cgatgagcgg agcgcctgct ccgactgctg
     6781 caggaacggc cctatcggca tcaccccggc gttgttgatc agcacgtcga tgcggccgcc
     6841 ggcctcgctg cgggccttgt ccagaaactc cgagaacgac tggcggttgg tcacatccaa
     6901 cgggtaaccc gacaccgggc ccatccggtc cagcgccgtt accgccgact ccagcgcgtt
     6961 gacgtcgcgg tcgccgatca ctacccgcgc tccgagccgc agcagcgcgt tcgccgtcgc
     7021 gtggccgatg cctcgcgcgg cgccggtgat cacgatggtc ttaccgttga tgttgcccat
     7081 gacgacgaac tttacaagtg ccaaattggc acatggaagg ggccgcgata aggatggcag
     7141 ccatgacgtt gatgcacctg gcggtggctc tcgagggata cggctggcac ccgtgggcct
     7201 ggcgatatgc gttggcacac aacacttctc ccgaggggca gctcaccggg cggtactggg
     7261 ccgcacgggt cgccgaagcg gagcgtggac tgctggactt cgtcaccttc gaggacacgc
     7321 cgcaggcgca aacaaggccg ctctctttgg gcggcgcaac gtgattgacg gtcaggacta
     7381 ttcgaagagg cggaagacgc ggtcgaagtg gcgcgccgac gaactggtgg tcacctcggt
     7441 cacccacctg cacgccgatc ggctgcgttc gcatgagttg atcgccgaac ggtggggcat
     7501 cacctgatga gcatccgaga gagcaagtac cgtaagcaga ttcacttagc agcgcacttc
     7561 cccggcgtca acaacactac tgtctggtcc gatactacag ccggcagtca gatcgacttc
     7621 gcttcgttcg tgcaccttgc gcagaccgcc gaacgcgggt tgttcgactt cctattcctc
     7681 gccgagggtt tgcgactgcg cgaacatcgc ggccatatcc acgaccccga cgtcgtcggc
     7741 cggcccggta ccggcgggcg gaggaattcc tcaccgtggc acgtaagttc tgggacagct
     7801 gggcaccgga cgccgtggtc gccgatgtgg acggcggtac gcccgcaccg cgggcgtttg
     7861 gccgagaccc gaaccagctc aaagtgtttc ccgcagcgac gttcgctctc ggggacacct
     7921 cgaaggaggc gcaagaccac gcggggcaca tccgcgagca gcaggcgagc ccaggcgaat
     7981 tcgccgccga catcgaccac tatgtggaat ccgatgcgtt ggcgggttca ttttcgttcc
     8041 gcacctgacc cacacgggct cgatgagttc gtgggcaggg tagtacc
//
LOCUS       DASWBF010000469         3778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1031595,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000469 DASWBF010000000
VERSION     DASWBF010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031595"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VF874_17170"
     CDS             complement(<1..737)
                     /locus_tag="VF874_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX9177724.1"
                     /translation="MAFGDGPDDAALRAAWENFCDQLKAAGERAFKDHNPSSALHRAD
                     AFRFLTQNLGQAFDLALETRDPRYPLIHAFCNPTRKLGGDCADFTYQQAWIDGESTYR
                     ITGNRGTARFFNITVQGPRPSGPGVLHEPFGDVPEANLFGAQLTTARDGSFELYVGGP
                     QRGRNWLPTTDGSRKLFVRQGFDSWDEWPARLRIEHVDMDSPKPLPTPATMIAAMGWA
                     GNFVTGLMDDWPEFPFTYGGVDAEHPNR"
     gene            complement(739..1515)
                     /locus_tag="VF874_17175"
     CDS             complement(739..1515)
                     /locus_tag="VF874_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX9177725.1"
                     /translation="MTDVTHGRLSVHNVTFYGATLTEFEKHWRALGLRRLSLLDSQLF
                     EPELLRAVDDGDYAVESVCHVFSSHDRLLQVIDAAAAVGARIIYMLTGGRGARTWEQA
                     AEQFCATIAPCVRSAEQAGVALAIETASSLYADIHIAHTLRDTIALAEMAGLGICIDL
                     FHCWAEADFAELVNRALPRTQLVQLSDYVLGDRALPARAVPGDGTIPIEPFVAQVLAG
                     GYEHGSDLELIGPRIDNEGRFDAARRACHVVSGMLNRIGV"
     gene            complement(1508..2671)
                     /locus_tag="VF874_17180"
     CDS             complement(1508..2671)
                     /locus_tag="VF874_17180"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9177726.1"
                     /translation="MTLDAAEMLARAEAETGLSDYGDPTLPSRFGVAVEHLNSSGLDA
                     DGRRHAADVCHWLLTSRLEFFEDRNRYPVAREVIESPMFVTGEPRSGTTLMHALMSVD
                     PTARALRFWEVMYPSPPPGVTGPDDPRRAKADADWREINAKMPKWLHSHPYNDMLGDG
                     LPEDERTWAFDFRVMTPTAWWRVPMQTVVGGLPTDPAPQYRIHQAMLQQCQYHRPRKH
                     WVLKGFHGSRLKEFFDAYPDAKLMWLHRDPVQVAASRTMMMADILAGIVGPVDLRAAA
                     KMHLELTRASIANTMTNPLVDDPRILHIRYTDFIADPVGTVRRYYEFSDWPLTAAAET
                     AMRDYLANNRGDRYGKFRYSTKLLTDIGEDLDALHDEFQPFRERFGVAIEKRD"
     gene            complement(2668..>3778)
                     /locus_tag="VF874_17185"
     CDS             complement(2668..>3778)
                     /locus_tag="VF874_17185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9177727.1"
                     /translation="AFFRTTLPLDLSQLARLDAGRYHSIWLPDHMVSFWPDSIWTPEF
                     TDLATASPSPHRNLDGMAVAAAAAVLTENVPIATSVVDTVRRHPSLLAQSALTIDHLA
                     RGRFILGLGSGETENTVPYGFDFNKPVTRFEEALKVIRLLWDSDGPVDFEGRFYQLHH
                     ARLDTEPYDGRFPQIWIGASGSRMIELAGRYADGWWPAGAWTPDHYAEMLGALRVSAE
                     RAGRDPMAITPCYIQVCVIGRDDAALAEILQAPLVKAFLLQVSAEALHNFGHEHPMGE
                     NWRGFQDIDPAVLTRDRIVDFLGRVDPEAILAMVPHGTPQQVARTIKSYVDAGLRVPK
                     ILDYGGMAGLQHAAASAEYVREAEDELMRLCGDVT"
BASE COUNT          636 a         1246 c         1256 g          640 t
ORIGIN      
        1 cggttcgggt gctcggcgtc gactccgccg taggtgaacg ggaattcggg ccagtcgtcc
       61 atcagcccag tgacgaagtt gcctgcccac cccatcgccg cgatcatcgt cgcaggtgtc
      121 ggcagcggct tgggtgaatc catgtccacg tgctcgatcc gcaatcgcgc gggccactcg
      181 tcccagctgt caaagccctg tcggacgaac agctttcgcg aaccgtccgt ggtgggcagc
      241 cagtttcgcc cgcgttgcgg cccaccgaca tacagctcga agctgccgtc ccgcgcggtg
      301 gtcagctggg cgccgaacaa atttgcctcg ggcacgtcgc cgaacggctc gtgcaacacg
      361 ccagggcccg aaggccgcgg gccttgcacg gtgatgttga aaaagcgcgc ggtgccccga
      421 tttccggtga tgcggtaggt cgactcccca tcgatccatg cctgctgata ggtgaagtcg
      481 gcgcagtcgc cgccgagttt gcgcgtcggg ttgcagaatg cgtggatgag cggatagcgc
      541 gggtctctgg tttccagcgc cagatcgaac gcctggccga ggttctgggt gaggaaacga
      601 aacgcatcgg cgcggtgcag ggccgagctt gggttgtggt ccttgaacgc ccgctcaccc
      661 gcggctttca gctggtcgca gaagttctcc caggccgccc gcaacgcggc atcgtcgggt
      721 ccgtctccga acgccatctc atacaccaat cctgttgagc atccccgaaa ccacgtgaca
      781 cgcgcggcgt gcggcgtcga accggccctc gttgtcgatg cgcggcccga tcagttcgag
      841 gtcggagccg tgttcgtagc ctccggccag cacctgcgca acgaagggct cgatcgggat
      901 cgtgccgtcg ccgggcacgg cccgagcggg aagtgctcga tcgccgagca cgtagtcact
      961 caactggacc agctgcgtgc gtggcagtgc ccggttcacc agctcggcga agtcggcttc
     1021 ggcccagcag tggaacaggt caatgcagat gcccagtccc gccatctccg cgagcgcgat
     1081 ggtgtcgcgc agcgtgtggg cgatgtggat gtcggcgtac agactcgacg ctgtttcgat
     1141 ggccaacgcg acgccagcct gctcggcgct gcgaacacag ggcgcgatcg tggcgcagaa
     1201 ctgctcggcg gcctgctccc aggtgcgtgc gccccgcccc ccggtcaaca tgtagatgat
     1261 gcgtgcgccg acagccgccg cggcgtcgat cacttgcaag agacggtcgt gcgaagaaaa
     1321 cacatggcaa accgactcca ccgcgtagtc gccgtcatcg acggctcgca gaagctcagg
     1381 ctcgaacagt tgcgagtcga gcaggctcag ccgcctcaaa ccgagggcac gccaatgctt
     1441 ctcgaattcc gtcagcgtcg caccgtagaa cgtgacgttg tgtaccgaca accgtccgtg
     1501 cgtcacgtca gtcacgcttt tcgatcgcga cgccgaaacg ctcacggaat ggctggaatt
     1561 cgtcatgcaa cgcgtcaagg tcttcgccga tgtcggtcag cagcttggtg gaataccgga
     1621 acttgccgta ccggtcgccg cgattgttgg cgaggtagtc acgcatcgcc gtctccgctg
     1681 cggccgtcag tggccagtcg gagaactcgt agtaacgacg gaccgtccca acaggatcgg
     1741 cgatgaaatc ggtgtagcgg atatgcagaa tgcgcgggtc gtccaccaga ggatttgtca
     1801 tcgtgttcgc gatgctcgcg cgggtcagct cgaggtgcat cttcgcggcg gcgcgcaagt
     1861 cgacaggccc gacgatcccc gcgaggatgt ccgccatcat catcgtgcgc gatgccgcga
     1921 cctgcacagg atcacggtgc agccacatca gcttggcgtc cgggtacgcg tcgaagaact
     1981 ccttcagccg ggagccgtga aaccccttga gtacccagtg tttccgcggc cggtggtact
     2041 ggcactgctg cagcatcgcc tggtggatgc ggtactgcgg ggccgggtcc gtcggcaggc
     2101 cccccaccac cgtctgcatc ggcacccgcc accaggccgt cggcgtcatc acccggaagt
     2161 cgaacgccca ggtgcgctcg tcctcgggca gtccgtcgcc cagcatgtcg ttgtacggat
     2221 ggctgtgaag ccatttcggc atcttggcgt tgatctcccg ccagtcggca tcggccttcg
     2281 cgcggcgggg gtcgtccgga ccggtcacac cgggcggcgg cgacgggtac atcacctccc
     2341 agaaccgcag cgcccgcgcg gtcggatcga ccgacatcaa ggcgtgcatc agcgtggtgc
     2401 ccgagcgcgg ctcgccggtc acgaacatcg gggactcgat cacttcgcgg gcgaccggat
     2461 agcggttgcg gtcctcgaag aactccaggc gtgaggtcag cagccagtgg cacacgtccg
     2521 cggcgtgccg gcgaccgtcc gcgtccaagc cgctgctgtt gaggtgctcg accgcgacgc
     2581 cgaaccgtga cggcaacgtc gggtcgccgt agtcggaaag tcccgtctca gcctcggccc
     2641 gggccagcat ttcggcggcg tcgagagtca tgtcacatct ccgcacagac gcatcagctc
     2701 gtcctcggct tcgcgcacat actcggcgga cgcggccgca tgctgcagtc cggccatccc
     2761 gccataatcc aggatcttcg gcacccgcag gcccgcatcg acgtaggact tgatggtccg
     2821 cgcgacctgc tgcggtgtgc cgtgcggcac catcgccagg atcgcttcgg ggtcgacgcg
     2881 gccgaggaag tcgacaatcc ggtcgcgggt caggaccgcc gggtcgatgt cttggaagcc
     2941 gcgccagttt tcgcccatcg gatgctcgtg gccgaagttg tgcaacgctt cggccgagac
     3001 ctgcagcaga aacgctttga ccagcggcgc ttgcaatatc tcggccagtg ccgcgtcgtc
     3061 gcgaccgatg acgcagacct ggatgtaaca cggtgtgatc gccatcgggt cgcggcccgc
     3121 gcgctcggcc gaaacacgaa gcgcgccaag catttccgcg tagtgatcgg gcgtccacgc
     3181 gccggccggc caccagccgt cggcgtagcg cccggcgagc tcgatcatcc gggaaccact
     3241 cgcgccgatc cagatctgcg gaaaccggcc gtcgtacggc tcggtgtcca accgggcatg
     3301 gtgcagctgg tagaaccggc cctcgaagtc gactggaccg tcgctgtccc agagcagccg
     3361 gatcaccttc agcgcctcct cgaagcgagt gaccggcttg ttgaagtcga acccgtacgg
     3421 gacggtgttt tccgtttcgc cgctgccgag gcccaggatg aagcggcccc tggcgaggtg
     3481 gtcgatcgtg agcgcgctct gcgcgagcag cgacgggtga cggcgcaccg tatccaccac
     3541 gctggtggcg atcggcacgt tctcggtgag cacggccgcg gcggccgcga ccgccattcc
     3601 gtcgaggttg cgatgcgggg acggcgacgc ggtggcgaga tcggtgaact cgggcgtcca
     3661 gatcgagtcc ggccagaagc tcaccatgtg gtcgggcagc cagatcgagt ggtagcggcc
     3721 ggcgtcaagc cttgccagct gagacaggtc cagcggcagg gtggtgcgga agaacgct
//
LOCUS       DASWBF010000470         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_305336,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000470 DASWBF010000000
VERSION     DASWBF010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305336"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2196)
                     /locus_tag="VF874_17190"
     CDS             complement(<1..2196)
                     /locus_tag="VF874_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9177728.1"
                     /translation="MVSIEALAAPIRSGLLERLGAAVRPEFRAEVLVPAVADPILGSP
                     ACAVPGCVRSSRYAGLCPAHLGRWRKAGQPDPHAWAATADPEVMGYRPLQSCLVPDCG
                     FGQHRYQLCYKHSRAWDKAGRPAVDQWKPQVAGTPAAVCAIPGCALWAELDAGWCSSH
                     HARWRQRGRPSAAEFIVYCASYGEDRFDLRPLRPQLRLEIGYALQCRVDLNRTRTTPR
                     SIKPLLDHLSATGAESLLERPLAEWLAGLPTAAALHTPRAFLGYAIECLLDLRDGTGW
                     DSEYQRDVWRLRRLGVSGHDGARLDFTAVQPVWLRDLAKRWCRWRMSCGVGLGQLRSD
                     RLAIVRLSQFTPGLASSSGPGVFDRAVLEAYLARLAVEIPHPKTRSAEIGCVTGFLHA
                     VRQHRWASLPAETQLYPSDQPRRDETPAPRAIPEFVMHQLESSANLDRISDPRIRLLV
                     EILIRTGLRIGDATRLQMDCLVRDPQGAVYLRYRNHKMRRDAVVPIDDELAAMIQEQQ
                     ARTRQRFPTTGVLLPRSSANPDGRLPIPTATFHLQLGQWLETCGVTDELGQSVHVTAH
                     QFRHTAATRWINHDVPQEVVRRLLDHTSHAMTAVYARLADTTIREQWERAQKINIRGE
                     PVDTTIDGPLADAEWMKQNLARAKMALPNGYCGLPLQKSCPHANACLTAVRHDRRIPT
                     PAPQAARRYPRADRPCRDRRPQSPRRDEPHGRNQPAHHHHHPRSRPTRLP"
     gene            complement(2190..3272)
                     /locus_tag="VF874_17195"
     CDS             complement(2190..3272)
                     /locus_tag="VF874_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9177729.1"
                     /translation="MPFGDPVSWTLVDQDSAVVEPVEGFLAHLHAVERSPNTVKAYAH
                     DLRDWFEFLDQCGVVWSRVRLEDVGRFVAWLRLPVAARVGNVAVLPTAESVCSEVTVN
                     RKLSAISAFYEFHQRHGVDLGDLLTTWQRRKTHGGSWRPMLAHLGSRPERGRRIRLRA
                     QRRIPDTLDGEQIAVIVAACDRLRDRFLFSLLASSGLRVGEAVGLRHSDVDAAARLVT
                     VVPRVNGNRARAKGGGRQVPVPGAVIRLYADYLHGEYGELDSDYVFVNLWSGPIGYPL
                     TYASVYDLVCRLRERTGIMFGPHTFRHSYATELLRRRVPVEVVQHLLGHASIATTSDA
                     YAHLKVEDARGALVAAGWLGDGDGSW"
     gene            3396..3545
                     /locus_tag="VF874_17200"
                     /pseudo
     CDS             3396..3545
                     /locus_tag="VF874_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611776.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          630 a         1246 c         1110 g          559 t
ORIGIN      
        1 cggcagtcgt gttggccggc ttcgagggtg gtgatgatgg tgagcaggtt ggtttcgacc
       61 gtgcggttca tctcggcgag gcgactgtgg ccgtcggtct cggcacgggc gatcagcgcg
      121 cgggtatcgt cgagctgctt gtggtgctgg ggtaggaatt cggcggtcgt gacgaacagc
      181 ggtcaggcac gcgttggcgt gcgggcacga tttctgtagc ggtagaccgc aatagccgtt
      241 gggcagtgcc attttcgcgc gggcaaggtt ctgtttcatc cactcggcat cggcgagcgg
      301 tccgtcgatc gtggtgtcaa cgggctcacc gcggatgttg atcttctggg cgcgttccca
      361 ctgctcgcga atggtggtgt cggccagccg ggcatagaca gccgtcatgg cgtggctggt
      421 gtggtcgagc aggcgtcgga ccacttcctg cggcacgtca tggttgatcc accgagtggc
      481 tgccgtgtgc cggaactggt gagcggtgac atggactgac tgaccgagtt cgtctgtgac
      541 accacaagtt tcgagccact gcccgagctg gaggtggaac gtcgcggtgg gaatgggcag
      601 ccgtccgtcg gggttggcgc tgctgcgcgg caatagcacg ccggtggttg ggaaccgttg
      661 ccgagtacgc gcctgctgct cctggatcat ggcggccagt tcgtcgtcga tgggcaccac
      721 cgcgtcgcgg cgcatcttgt ggttgcggta gcgcaaatag accgcgccct gcgggtcgcg
      781 gaccagacag tccatctgaa gccgggtggc gtcgccgatg cgcaggccgg tgcggatgag
      841 gatctccact agcagccgga tccgcgggtc gctgatccgg tcgaggttgg ctgagctttc
      901 cagctggtgc atgacgaact cgggtattgc ccgcggtgcc ggtgtctcgt cgcggcgggg
      961 ctggtcgctg ggatacagct gcgtctcagc cggcagcgat gcccaccgat gctggcgcac
     1021 tgcgtggaga aacccggtca cgcagccgat ctcggcgctg cgggtcttcg ggtgcgggat
     1081 ctcaacggcc agtcgtgcca ggtaggcctc cagcaccgcc cggtcgaaca cgcccggtcc
     1141 cgacgagctc gctagtccgg gcgtgaactg cgacaaacga acgatcgcga ggcggtcact
     1201 gcgtagctgc cccagcccga ctccgcacga cattcgccac cggcaccacc gcttggcaag
     1261 atctcgcagc cagactggct gcaccgcagt gaaatccagg cgggccccgt catggccgga
     1321 aacaccgagc cgccgtagcc gccacacgtc acgctggtat tcgctgtccc agccggtccc
     1381 gtcgcgcaaa tcgagcaggc actcgatcgc gtaaccgagg aacgcgcgcg gggtgtgcag
     1441 cgcggccgcc gtcggcagcc cagccagcca ttcggccagc ggccgctcca acagcgactc
     1501 ggccccggtt gccgacaagt ggtccagcag cggcttgatc gatcgcggtg tggtgcgggt
     1561 tcggttcagg tcgacgcggc attgcagcgc gtagccgatc tccagccgca gctgcggccg
     1621 cagcggccgc aagtcgaaac gatcctcgcc gtagctggcg cagtagacga tgaactccgc
     1681 cgccgacggg cggccacgct gtcgccaccg agcatgatga gagctgcacc agcccgcgtc
     1741 cagttcggcc cacaacgcgc accccgggat ggcgcacacc gctgccggtg tgccggccac
     1801 ctgcggcttc cactgatcca ccgctgggcg tccggccttg tcccaggcac gagaatgctt
     1861 gtagcacaac tgataccgat gctgcccgaa gccacaatcg ggcaccaggc acgattgcaa
     1921 cggccggtaa cccatcacct ctggatcagc ggtcgccgcc cacgcgtgcg ggtccggttg
     1981 accagccttt ctccatcggc ctagatgggc cgggcagagc ccggcgtagc gactcgaacg
     2041 cacacacccg ggcaccgcgc acgcgggcga cccgagaatc ggatccgcca cggcgggcac
     2101 cagcacctcg gcccggaact ccggccgcac cgccgcaccc aaacgctcga gtaggcctga
     2161 tctgatcggc gccgccagcg cctcgatgct caccacgacc cgtccccgtc acccagccac
     2221 cctgcggcca ccaacgcgcc gcgcgcgtcc tcgaccttca gatgcgcgta cgcgtcactg
     2281 gtcgtagcga tcgacgcgtg acccaacaga tgctgcacca cctcgaccgg cacccttcgg
     2341 cgcaacaact ctgtcgcata tgaatggcga aaagtatgcg gcccgaacat gatcccggtc
     2401 cgctcccgca atcggcagac cagatcgtag acgctggcat acgtcaacgg atacccgata
     2461 ggacccgacc ataagttgac gaacacgtaa tcgctgtcca gctcgccgta ctcgccatgc
     2521 aggtaatctg catacagccg gatcacggcg cccggcaccg gcacctgtcg acctccgccc
     2581 ttggcccggg cccgattccc attcaccctc ggcaccacgg tcaccagccg ggccgcggcg
     2641 tcgacgtcgc tgtgccgcaa ccccaccgcc tcaccaaccc gcagccccga tgaagccagc
     2701 agggaaaaca ggaaccgatc ccgcagccga tcgcaagccg ccacgatcac cgcgatctgc
     2761 tcaccgtcta gcgtgtcggg gatgcgccgc tgcgcccgca accggatccg cctgccgcgt
     2821 tcgggtcgag aacccaagtg cgccagcatc ggtcgccacg atccaccatg cgtcttgcgc
     2881 cgctgccagg tcgtcagaag atcccccaga tccacgccgt gacgctgatg gaactcgtag
     2941 aacgccgaga tcgccgacag cttgcggttc accgtcacct ccgagcacac gctctccgcg
     3001 gtcggcaaca ccgcgacatt gcctacccgc gccgccaccg gaagccgcaa ccacgccacg
     3061 aaccggccca cgtcttccag ccgcacccgc gaccacacca cgccgcactg atcgaggaac
     3121 tcgaaccaat cccgcagatc atgggcatag gccttcaccg tattcggcga gcgctcgacc
     3181 gcgtgcagat gcgccaagaa tccctcgacc ggctccacca ccgccgaatc ctgatcgacg
     3241 agagtccacg acaccggatc gccgaacggc atcaccaccg gctgcacccg cagaccagga
     3301 cacgtcacag acaccgaaga taaccggtcc acaacgcgtt tcccggcacc ctgaacggcg
     3361 tgtcgtctac atcgtggacg gtctcatcgg ggcctcagat aaccatcgaa atcctcaagg
     3421 ccgcaacaag tttcttcgcg cgggagtgcg acccgcgaca ccgctgatct gtcagttcat
     3481 cgacgagcac aagggtacct acggggtcgt accgatctgc cgggcactgg ccgtgcacgg
     3541 cgtgc
//
LOCUS       DASWBF010000471         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_635027,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000471 DASWBF010000000
VERSION     DASWBF010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635027"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1121
                     /locus_tag="VF874_17205"
     CDS             117..1121
                     /locus_tag="VF874_17205"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9177730.1"
                     /translation="MKSTDVIGRSAHALLSLPNAVLAPLAGPRPTIDGQRLTPRLQLL
                     ANGGKRALAGLSFPPTALERRFDAVGQWLTLAHGDDASWRDLSAGGRRARLFEPMEPT
                     TGLVVFVHGGGWQFGSIDASHGVCAFLSKTTGLKVLSLDYRLAWQAQFPAAFDDVVAG
                     FREAVDRADELGVARDRIAIAGDSAGGNIASAAALALGADPTYRPKLAALIYPVVDGD
                     LDRYESTHLFHAPLDRAIVHRDMRRYAPDDADRLDPRFAVMAASDLSAMPPAYIATAG
                     MDVLRDQGEAFGERLRESGVDASVQRFPNLPHGFLNLLVDPDAERAAAEIGRAIGERV
                     "
     gene            1326..1925
                     /locus_tag="VF874_17210"
     CDS             1326..1925
                     /locus_tag="VF874_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9177731.1"
                     /translation="MAATLVGYCRCSTDAQDLTAQRQRLREFGVNEDRIYLDHGLTGT
                     NRARPGLDQALAALREGDTLVVPKLDRLARSVPDARDIGDGLAARGIKLSLGGQVYDP
                     TDPMGKMFFNILATFAEFEVDLLRMRTREGMAVARAKGRLKGRRPKLSTTQQTELRRM
                     HGTGDYTITDLAELFSISRPTVYRALAAVVTVAAQRNLR"
BASE COUNT          354 a          726 c          649 g          322 t
ORIGIN      
        1 tgaaggaagc cggaacggac gatcctgatc gggcatgctg gagaagctca tgcacggcga
       61 cgtggcgtcc agaagacgat cctgaaaaat caaactcttg attggatcgg agggcaatga
      121 agtcgaccga tgtcattggc cgtagcgccc acgcgctgct gtcactcccg aacgcggtgc
      181 tcgcgccgct cgccggacca cgcccgacga tcgacggcca gcggttgact ccgcgcctgc
      241 aactgctggc caacggcggg aagcgcgcgc tcgccgggct gtcatttccg ccgaccgcgc
      301 tggagcgcag gttcgacgcc gtgggtcaat ggctcacgct cgcgcacggc gacgacgcga
      361 gctggcggga cctgagcgcc ggcggacgcc gggcacggtt gttcgagccg atggaaccga
      421 cgacgggcct ggtggtcttc gtccacggcg gtggatggca gttcggcagc atcgacgcct
      481 cccacggcgt atgtgcgttc ctctcgaaaa ccactgggct gaaagtgctt tcgctcgact
      541 accggctcgc ctggcaagcg cagttcccgg ctgcgttcga tgacgtcgtt gccggtttcc
      601 gagaggcggt cgatcgcgcc gacgagctcg gcgtcgcgcg cgaccggatc gcgatcgccg
      661 gcgacagcgc cggcggaaac atcgcttcgg ctgccgcgct ggcgctcggt gccgacccga
      721 cctaccggcc caagctcgct gcgctgatct atccggtcgt ggacggcgat ctcgaccgct
      781 acgaatccac gcacctgttc cacgcgccgc tcgaccgggc gatcgtgcac cgcgacatgc
      841 gccgctacgc ccccgacgat gccgatcgcc tagacccgcg cttcgcagtc atggcggcaa
      901 gcgacctctc ggcaatgccg cccgcttaca tcgccaccgc tggaatggac gtcctgcgcg
      961 accagggcga ggccttcggc gagcggctgc gcgagtcggg cgtggacgcg agcgtgcagc
     1021 ggttcccgaa cctcccgcac gggttcctca acctgctcgt cgatccggac gccgagcggg
     1081 ccgccgccga gatcggccgc gcgatcgggg agcgcgtctg atcacagccg ggtggtgccg
     1141 aggttcggcg ttgcccgctc cgtcgccgac ctctgttcgt tagccgaccc ggctaactaa
     1201 cggacatcga ccacggggcc gccgatcgca atcacgacgt gccgcgacag ccgccttcgg
     1261 ctgtctcaga tacctggtac gcaaacaatc tcagtgacgg atggctatcc gttacagtcg
     1321 aacgaatggc cgccactctc gtcggctact gtcgatgctc gaccgacgcg caggacctca
     1381 ccgcgcagcg gcagcggttg cgggagttcg gcgtcaacga ggaccggatc tacctcgacc
     1441 acggactcac cggcaccaac agggcgcgac ccggcctcga ccaggcgctc gccgccctcc
     1501 gtgaaggcga caccctcgtc gtaccgaaac tcgaccggct cgcccgctcg gtcccagacg
     1561 cgcgcgacat cggcgacggg ctcgcggccc gcggcatcaa gttatccctt ggcggccagg
     1621 tctacgaccc gaccgacccg atgggaaaga tgttcttcaa catcctcgcc accttcgcgg
     1681 aattcgaagt cgacctgctg cgcatgcgca cccgcgaagg aatggccgtc gcccgtgcga
     1741 aggggcggct caagggccgg cgaccgaagc tgtcgaccac gcagcagacc gagttgcgcc
     1801 gcatgcacgg caccggcgac tacaccatca ccgacctcgc tgaactgttc agcatctccc
     1861 gacccaccgt ctaccgggcc ctcgcagccg tggtaactgt tgctgcgcaa cgcaacctca
     1921 gatagcgcct ggtcctactt gcatatgggt aaacatcggg agcctccgaa cgccgacgag
     1981 cagttagcgt tagcacgctg ccgctcaacg ttcccgacga agttgcctga tgctgggcca
     2041 attccgaata c
//
LOCUS       DASWBF010000472         3379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_782561,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000472 DASWBF010000000
VERSION     DASWBF010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782561"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..608)
                     /locus_tag="VF874_17215"
                     /pseudo
     CDS             complement(1..608)
                     /locus_tag="VF874_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018159723.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            678..1271
                     /locus_tag="VF874_17220"
     CDS             678..1271
                     /locus_tag="VF874_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9177732.1"
                     /translation="MVNPRATNEDLTAKARIRNAALDLYSQYGEDRVSLRAVAAEAGV
                     TLGLVQHHFKTKAGLREAVDQLIVDYFAAAIAEVPADGAPAEVTAARNEAVRRMLEEN
                     PPVVNYVRRALLEPSDSHLHLLDVLVELTSREVGALRKSGRASTKRRESTQVVAVFAQ
                     QMGELLLAPLVDAVWDRVAVPGDQHKPRLRVSVDAGD"
     gene            1382..3130
                     /locus_tag="VF874_17225"
     CDS             1382..3130
                     /locus_tag="VF874_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9177733.1"
                     /translation="MQNSSPTRPRTLCEAFQRTAAQFPDRVALRTADGGVEVTWRQYR
                     DRVQSIATGLAGLGVGPGDTVALMMTNRPEFHLCDTAVLHTGATPFSIYNTNPPEMVS
                     YLFDNAENRVVICEQQFLPVVSAAMASGGKVEHVICVDGSPERTIGLAELERRPSPGF
                     DFDQAWRSVQPDDVLTIVYTSGTTGPPKGVELTHTNFIENARITEEFGGMSFEDRAIS
                     YLPDAHAANRWFTHYQNLLYGVQISTLDDAKQILDALSSVRPTLFLGVPRVWIKAKAG
                     LEAAVAQERPLRRALAGWAFAAGRSPARGPLATLRRALADRLVLSAVRARLGLDQARI
                     AVTGAAPIPAEVHAFILGLGITLCEGYGMSECTAGATVHRPGDIRVGTVGTPLPGVEV
                     RVAADGEILVRGDNVMRRYRKDPDKTAEAIDADGWLHTGDIGEFDADGHLRVIDRKKE
                     LIINAAGKNMSPTNIENAIAANTPLAGPVMAIGDARPYNTALITLDPDEVTKIDGSKT
                     IAQLATDSRVHEAIDAGIKAANAQLSRVEQIKKFTVLPDVWEPGSPHLTPTGKLKRKP
                     IVATYADVIEAMYSQP"
BASE COUNT          588 a         1150 c         1118 g          523 t
ORIGIN      
        1 cgggaacgcc ccgcccagtg gggccccgtc acccagcagg ccgtagcgca ccgcgtcgcc
       61 ggcccgggcg gcggctttct ccagtgccac ccggtaacgc ccgcccgccc cggtggtgtc
      121 gcggcggtgc agcgcacaca gggccgtcgt caccgcgcgc tcggcgccca gcacgtcggc
      181 gatcggcacc ttcggcagct gcggtgggct cggcgtgccg tgcactgcct ggtaattgag
      241 gtcatggccg ggggtctcgg cggcgtcgcc ggcgtggccg acgatctcga tcagcgacag
      301 gcgggaaaat acgttgatgg ccaaccggtc cagcgcggac ggccgcatgg aggtgatcag
      361 caggtcggcg tcggccagca gctcgtcgaa cttcgcacgg tcgtcgaggt ctttgaggtc
      421 gaggccgacg atgtgctggc ccgtcgtgag cgcggcgtac caggccggcg cggccgtggc
      481 cagcggatca ccagcgggcg gctcgacctt ggtcaccgtt gccccaaact cccgcgcagg
      541 cgggcggcgg ccagggggcc gggcaggttc accgtcagcg atacgacgga gacatcgtca
      601 agcacgatac aactgtatcg tgccctggtt atttgtaaag tacgctggtc ggtagcgtta
      661 ctgggcgagg aggcgacatg gtgaatccgc gggcgacgaa cgaggacctg accgccaagg
      721 cgcggatccg caacgccgcg ttggatctgt actcgcagta cggcgaggac cgggtgtcgc
      781 tgcgggctgt cgccgccgag gccggggtga cgctgggcct ggtgcagcac catttcaaga
      841 ccaaggccgg gctgcgcgaa gcggtcgacc aactgatcgt ggactacttc gccgcggcga
      901 tcgccgaggt acccgccgac ggcgcacccg cggaggtcac cgcggcgcgc aacgaggcgg
      961 tgcggcggat gctcgaggag aacccgccgg tggtgaacta cgtgcgccgc gccctgctag
     1021 agccctccga cagccacctg catctgctgg acgtcctggt cgagttgacc agtcgcgaag
     1081 tcggcgccct gcgcaagtcc gggcgcgcgt cgacgaagcg tcgggagtcc acccaggtcg
     1141 tcgcggtgtt cgcgcaacaa atgggggaac tgctgttggc gccgctggtc gatgccgtgt
     1201 gggaccgggt cgccgtaccc ggtgaccagc acaagccgcg gttgcgggtc agtgtcgacg
     1261 ccggcgacta accgcagcgg tcgggtgagg cggtcatcgc tgccgaccat cacgatccgc
     1321 cccttgcctt gatacggcca tatgggcaac aatacaactg tatcgctctg gagggagggg
     1381 cgtgcagaac tcatcaccga cccggccgcg cacgctgtgc gaggcgtttc agcgcaccgc
     1441 cgcgcagttt ccggatcggg tggcactgcg cacggccgac ggtggggtcg aggtgacctg
     1501 gcggcagtac cgagatcggg tgcagtcgat cgcgaccggg ttggccgggc tcggtgtcgg
     1561 accgggcgac accgtcgctc tgatgatgac caatcgtccc gagttccacc tgtgtgacac
     1621 cgccgtgctg cacaccgggg caacaccttt ttccatctac aacaccaacc cgcctgagat
     1681 ggtgagctat ctgttcgaca acgccgagaa ccgcgtcgtc atctgcgagc agcaattcct
     1741 gcccgtcgtt tcggccgcca tggcttccgg cgggaaggtg gagcacgtga tctgcgtcga
     1801 cggttcgccg gaacgcacca tcggactggc cgaattggaa cggcggccgt cgccggggtt
     1861 cgatttcgac caggcgtggc ggtcggtgca gcccgacgac gtgctcacca tcgtctacac
     1921 ctcggggacg acgggaccac ccaagggtgt ggagctaact cacacgaact tcatcgagaa
     1981 tgctcgcatc accgaggaat tcggcggaat gagcttcgag gatcgcgcga tctcgtacct
     2041 tcccgacgcg cacgccgcca accgctggtt cacccactat cagaatctgc tgtatggcgt
     2101 gcagatcagc accctcgacg acgcgaaaca gattctcgac gctctcagct cggtccggcc
     2161 cactctcttc ctgggcgtgc cgcgggtctg gatcaaggct aaggccggcc tggaagccgc
     2221 cgtcgcacag gagcgcccac tccgtcgtgc actcgccggc tgggcattcg ccgcgggacg
     2281 cagcccggca cgcggtcccc tcgccacgct gcgacgagca ctcgccgacc gactggtgct
     2341 ctcagcggtg cgggcccgtc tgggactcga ccaggcccgc atcgcggtca ccggcgcggc
     2401 acccataccc gccgaggttc acgccttcat cctgggcctg ggcatcaccc tgtgcgaggg
     2461 atacggcatg agcgaatgca ccgccggcgc cacggtccac cgccccggcg atatccgggt
     2521 gggcaccgtc ggcacgccct tgccgggcgt cgaggtccgg gtcgccgccg acggcgaaat
     2581 cctggtccgc ggcgacaatg tcatgcgccg ctaccgcaag gaccccgaca agaccgcgga
     2641 ggcgatcgac gccgacggct ggctgcacac cggcgacatc ggtgaattcg acgccgacgg
     2701 acacctgcgc gtgatcgacc gcaagaagga actcatcatc aacgcggccg gtaagaacat
     2761 gtcgcccacc aacatcgaga acgcgatcgc cgccaacaca ccgctggccg gaccggtcat
     2821 ggctatcggc gacgcccgcc cgtacaacac cgcgctgatc accctcgacc ccgacgaagt
     2881 caccaagatc gacggcagca agacgatcgc gcaattggcc accgacagca gggttcacga
     2941 ggccatcgac gccggaatca aagccgccaa cgcacaactc tcacgcgtcg agcagattaa
     3001 gaaattcact gtcctgcccg atgtatggga accgggcagc ccacacctta cgcccaccgg
     3061 caagctcaaa cgcaagccca ttgtcgccac ttacgccgat gtgatcgaag cgatgtactc
     3121 ccagccttga tcgccctggc atcgtcaagt tgttgacctt gtgtggtggc tgcgagggtt
     3181 ggtcgttgtg gcgattgcgg cgaggtactg ccagatccgc cacaagcagc tgcttccaca
     3241 gcatggtccc ctctagcacc gcagtcggcg cacgcgccgg caaagtccgc gctgcccatc
     3301 gcgtcacgaa ctgtcgacgc cgactgggtt tcatgctcgg ggctatctga gggcagcaga
     3361 gcccgtcaac gccgcgtcg
//
LOCUS       DASWBF010000473         8933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1304225,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000473 DASWBF010000000
VERSION     DASWBF010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304225"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..577)
                     /locus_tag="VF874_17230"
                     /pseudo
     CDS             complement(265..577)
                     /locus_tag="VF874_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            670..1143
                     /locus_tag="VF874_17235"
     CDS             670..1143
                     /locus_tag="VF874_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177734.1"
                     /translation="MLALLRVSTPATAVVETSEEDWYANLVWVDRRKCLLVTHAGTLF
                     SVFVPNVTAAGLRRIGPLVVSAIETALRAEGLPVDTLGELDAQEVTVAKTADRRILGT
                     MNDLTVTAEHVVGTAGGLARCDIDALNHALHRTINSITGYVPPIELVTASRGDQR"
     gene            1140..1343
                     /locus_tag="VF874_17240"
     CDS             1140..1343
                     /locus_tag="VF874_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177735.1"
                     /translation="MSYVVLAHGSTQSAAGLWRLTGASSRRGHRALTVEVPRAAADTS
                     IEYAELLAAQLPADLHGTGIRHA"
     gene            1516..2106
                     /locus_tag="VF874_17245"
     CDS             1516..2106
                     /locus_tag="VF874_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9177736.1"
                     /translation="MAGTLIGYARCSTDAQDLTAQRDRLRELGVTENRIYLDHGLTGT
                     NRKRPGLDQALAAVRAGDTLVVPKLDRLARSVPDARAIGDDLAARGIKLSLGGQLYDP
                     TDPMGKMFFNILATFAEFEVDLLRMRTREGMAVARAKGKLKGRQPKLSAKQQNELRRM
                     HGSGDYSITDLAELFKVSRPTVYRTLAESNSQTKAT"
     gene            complement(2188..2466)
                     /locus_tag="VF874_17250"
     CDS             complement(2188..2466)
                     /locus_tag="VF874_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177737.1"
                     /translation="MSIETVDTHAHAAAAVALVRHRAHQTRFLPIAARPVPDHPGYFQ
                     VNRVDRTGRALPGGWTFLVGLTKPTVVRSSGDPDRLDFAAGLRRGTRA"
     gene            complement(2624..3157)
                     /locus_tag="VF874_17255"
     CDS             complement(2624..3157)
                     /locus_tag="VF874_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177738.1"
                     /translation="MGADRELDPVGDEVVDAQVVEAVKREVARELRAGVRSWRGPIPP
                     GSELQAIDFVVPGGADRVLRMSEKALDSQIEVDTTLAHGDVASVRRGQWQSTVVVAGS
                     VVCALVTALLKAPWEVSVAFLAAPVFEFGSSLVRAIREPRRDSDRRDGDRQRDRDSDR
                     RDGDRQRDTARHKLKRS"
     gene            complement(3691..4017)
                     /locus_tag="VF874_17260"
     CDS             complement(3691..4017)
                     /locus_tag="VF874_17260"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX9177739.1"
                     /translation="MSEKYAFIDAERAAAAQAAKLPTVARMCALLEVSKSGFYEWRGR
                     PVSAAQRRRELLAEKIGVLFEAFDGTYGYRQIHAELLRAGERVGDELVRKLMRELNLS
                     AVQPKP"
     gene            complement(4476..5375)
                     /locus_tag="VF874_17265"
     CDS             complement(4476..5375)
                     /locus_tag="VF874_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006438145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9177740.1"
                     /translation="MGEAVAAYLATLDHPESAGTRRVYAATLRALRTAFDAQTPLVVL
                     AEPAQVQTLTGWFEQSWGHKAAATYNRNLDALRSALHYWSDQDWIHGDPTRSLRRRGR
                     APDRSKGLARELVDQLLTRDDLALRERVLWRMLYETAARAREILALDIDDLDRRNRSA
                     KVRRKGGAVDTVIWQTGTARLLPRLIGTRTSGPLFLTDRAARVQLPPADLDPSSGRAR
                     LSYRRAAELFEHATTGIPSGPWTLHQLRHSALTHAAEDGANTATLLAYSGHTSVTSLA
                     RYTRLSPEALSRWQQTRDTARRR"
     gene            5591..7129
                     /locus_tag="VF874_17270"
     CDS             5591..7129
                     /locus_tag="VF874_17270"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HEX9177741.1"
                     /translation="MPVIVDPAALAGYAAFPADLDRDWVGSVCHLSVDDLRAAGRRVD
                     ETTRLGFAVQLVTVRAIGTFLADPTAVPDPVLAAVARQLDIADPVVLHRYRDLPVRWR
                     HTAEIRDRYGYRDFTAQPARFLLTLDLYRQAWSDDVAASVLFRAAHRRLLAEQILLPG
                     HSVLARLVTAVRDRAARRLHIRLAAAAPPDLVARLQNLLIVPDGARRSELDRLRRPPF
                     TPTITGLVKALQRLAEVRALGAGAVDLCGLPARRVAALARYADQAWVTQLADLADERR
                     VATLLAYTHALTADARDDVIDIFDVVFGDLQRSATHRGQQRRVGELRDYDRAVGQLHT
                     RMRSILDVLDADPYSQTAVLDGLRADRDGIEAAMGTVSALMRSPADPFHERLVAAYPQ
                     IRRFLPLLIEAIKLDATRVRTTGAGRLPRSRRLAYQQTAHHQTPRHRAAVGGDQCRLV
                     AARARHRRGHRQPVRLCLLCARCVADPVASPRHLRTREHPLGRPPRRTPDSRRVGGAA
                     RNPL"
     gene            7909..8598
                     /locus_tag="VF874_17275"
                     /pseudo
     CDS             7909..8598
                     /locus_tag="VF874_17275"
                     /inference="COORDINATES: protein motif:HMM:NF013676.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            8715..>8933
                     /locus_tag="VF874_17280"
     CDS             8715..>8933
                     /locus_tag="VF874_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177742.1"
                     /translation="MRVVKIGVDSATSSRLDELRHKYRRFPYDSTFNQLYNAERFDMY
                     RDLGFDSTSRAIRARLDESPSAGGDPTNS"
BASE COUNT         1491 a         3137 c         2808 g         1497 t
ORIGIN      
        1 aacgcccacg tcaaacgatc gctcgtcgca tacgaccact gaccccggtt gggaatcact
       61 catctagcaa cggccgccga catcgaggaa tggttcacag ccgatagtga tttggcaacc
      121 acacagtccg cgaactgtgt catccgagac atgatctgat cactccgctt tctttattac
      181 cgggactctt tacaaccggg acagcggcca ccgagggtac gggggtgtct ggtcgaggct
      241 gaaaatgtgt gctcgggaag cctgtcagca gccggtggct gctcggagtg tgcggcaggc
      301 ctggtgcaga cgtgctgaag acaagcgggt gatccggcgt cggaacgtgg cgcgcggcag
      361 cgtgtaaatg ttgtcgaagt tgacgacgca atcgcttggc acgcggccct cggcggcggt
      421 cagttccagc tcagacacca gtcagtcctc tacgggtgcg ggtcaatgcg gctacgacca
      481 ccgcgccgat ccggtcgcca acagggtctc tggtgagaag gagcacgggc cggtcgccgc
      541 cgggagtcgc ggcaaaccag agttcaccgc gtcgcatcgg cttccgctcg cgacgggacg
      601 tgttgcgacg accgccagaa tccgctgatc tctacgatcg tgaccgtgat gctgcgatgc
      661 accggcaagg tgttggcgct gttgcgggtg tccacgccgg ccacggcggt cgttgagacc
      721 tccgaagagg actggtatgc caacctggtc tgggtcgatc ggcgcaagtg cttgctggtg
      781 acccacgcgg ggacgttgtt ctcggtgttc gtgccaaatg tcacggccgc cgggctacgt
      841 cgaatcggtc cgctcgtcgt ctcggcgatc gagaccgccc tccgcgctga agggctgcct
      901 gtcgatacgc tcggtgagct cgatgcccag gaggtgacgg tggccaagac cgccgaccgc
      961 cgcatcctgg gcaccatgaa cgacctgaca gtcaccgccg agcatgtcgt cggcactgct
     1021 ggaggtctgg cccgctgcga catcgatgcg ctcaaccacg ctctacatcg cacgatcaac
     1081 agcatcaccg gctatgtccc gccgatcgag ctggtcaccg ccagccgtgg ggatcagcgg
     1141 tgagctacgt ggtcctggcg cacggcagca ctcagtccgc ggccgggttg tggcgactca
     1201 ccggggcttc gtcccggcgc ggacaccggg cgctgaccgt agaggtgccc agggcggccg
     1261 cggacaccag catcgaatac gccgagctgc tggccgcaca gctgcccgcg gatctgcatg
     1321 gcaccggcat acggcacgct tagccgcact cattagtgcc gacaagaccg ccgctggttg
     1381 gcgtccgttg aaggaacccc aaacggacac ccgataccga cagccgggcg ccgtccgcga
     1441 cttcgaccag cagtgtctca catacctgta cgaaactatt ctcagtgatc ccccggcatt
     1501 cgttacacta cgatcatggc cggaacgctg atcggctacg cgcgctgctc caccgacgcc
     1561 caggacctca ccgcgcagcg tgaccggctg cgtgagctcg gcgtcaccga gaaccggatc
     1621 tacctcgacc acggcctgac cggcaccaac cgcaaacggc ccggcctgga ccaggccctc
     1681 gccgccgtcc gcgccggtga caccctcgtc gtcccgaagc tcgaccggct cgcccgctcg
     1741 gtgcccgacg cacgcgcgat cggcgatgac ctcgccgccc gcggcatcaa gctgtctctc
     1801 ggcggacagc tctacgaccc caccgacccg atgggcaaga tgttctttaa catcctcgcc
     1861 accttcgccg aattcgaggt cgacctgctc cgcatgcgca cccgcgaggg catggctgtc
     1921 gcccgcgcga agggcaaact caaagggcgc caaccgaagc tgtcggccaa gcagcaaaac
     1981 gaattgcgtc ggatgcatgg cagcggcgac tacagcatca ccgatctcgc cgagctcttc
     2041 aaagtctccc ggccaaccgt atatcgcaca ctggcagagt cgaattcaca gacgaaggct
     2101 acgtagccac tggcgcgcga gctcggcgcc ttcctcgatt gcctctcatg acacggttct
     2161 taccggcacc ccttgttggg cagacggtta cgcgcgggtg ccgcggcgca ggccggcggc
     2221 gaagtcgagc cggtcggggt cgccgctgct gcgtaccacg gtcggtttgg tcaggccgac
     2281 caggaaggtc cagccgccgg gtagtgcgcg gccggtgcgg tcgacccgat tcacctggaa
     2341 ataccccgga tggtcgggca ccggtcgcgc cgcgatcggc aggaagcggg tttggtgggc
     2401 gcggtgtcga accagcgcca ccgccgcggc ggcgtgggcg tgcgtgtcaa cggtttcgat
     2461 gctcatggag tcggtcacct cccgcgggtg acggtagctg cgaaagtcga gttcggccca
     2521 agactcccga agctggcctg agccgcaagg taggtcagtt ggcggcttgg tctcgttgga
     2581 gatacgcgtg cgccaagtgc gcacagcgac tagccgagtg gctttaactt cgctttaact
     2641 tgtgtcgcgc tgtgtcgcgc tgccgatcgc cgtcgcgccg atcgctgtct ctgtcgcgct
     2701 gccgatcgcc gtcgcgccga tcgctgtccc ggcggggctc tctgatcgcc cgtacaagcg
     2761 acgaaccgaa ctcgaacact ggcgctgcta ggaacgccac cgaaacctcc catggcgcct
     2821 tgagcagagc cgtcacaagc gcacacacca ccgatcccgc caccacaaca gtcgactgcc
     2881 actggccgcg tcgcaccgag gccacatcgc catgagccaa cgtggtgtcg acctcgattt
     2941 gcgagtcaag cgccttttca ctcatccgca agacgcgatc ggcaccacca ggcaccacga
     3001 agtcgatagc ctgcagctcc gaaccagggg gtatcggccc acgccaggag cgcaccccgg
     3061 cccgcagctc gcgggccact tctcgtttga cagcctcgac tacctgggcg tcgacgacct
     3121 catcgccgac cggatcgagt tccctgtccg ctcccacgcc cggcggatct gcgcggtcga
     3181 gcgcgtttcc gcgacccgcc tctcggtccg cagcgggcgc ggatggatcg tcatcgtgtc
     3241 gacgccccgc tcgcgcgctc gggccatcgc tgccctggtc gtcgtgttgc ggctcatgag
     3301 ccaacgtcac cctttcctgc caccactcgc gatggtaacg gcaaaccctg gctcgtggca
     3361 cggcatggca cggcttagcc cgttcgtgac atggcatggc cgcgcggcgt ctttcaccac
     3421 tcttctcagg ccgcacccag tgcgacgacc gcgacccgct gaccgccgca cctcggcccc
     3481 ctaatcggcc tgtgatgagg attggaacag gatcctgaac gcgcggtatc tcaggaacct
     3541 tcacgacagg tagtaatcgg tggcgtctgg cccggtggtg gtggcaggtc ccgaactggc
     3601 catagccccg ttatgcggag agctaccacg tcgaggtaag tatcggccgg gtgggccgat
     3661 tcggtcggcc aaatggtgga ccgacgaagt tcacggtttc ggttgcaccg cagacagatt
     3721 cagttcccgc atgagcttgc gtaccagctc gtcgccgacg cgttcgccgg cgcggagcag
     3781 ctcggcgtgg atttgccggt agccgtaggt gccgtcgaag gcctcgaaca ggacaccgat
     3841 cttctcggcg agcagctcgc ggcgccgttg agcggcgctg accggccggc cccgccactc
     3901 gtagaagccc gacttggaca cctccagcag cgcgcacatc cgcgcgaccg tcgggagctt
     3961 tgccgcctgc gcggcggcgg cgcgctccgc atcgatgaac gcgtacttct cgctcaccgt
     4021 tgctccctcg cgaagtacgc aaggagccgc cgcttttttt aggaacgcga gttccatcgc
     4081 catttcccgg ctttctcgtt ctagttcgcg tagccgcgct cgctcggata attgcagcgg
     4141 cggctcgtcc tctgcatgct cttcccggta ctttcggacc caactaccga ctgtggtatc
     4201 gctgagccca tgatcacgag cgacccgcgc gataggctgt tgcccctcca ccaccagctt
     4261 ggctatttcc tcccgaaact caggagagaa tttctcgtat ctcttcgcca attcccatcc
     4321 tcctttccga aacaaaccct attgtccgca gtcctgaaag ctgagggcac tccaagataa
     4381 cttgacagtc atgaagaaga agaagcagca gcgtgtggtg cggcccaagt cgtgaagacg
     4441 ctggcagtaa ctgcgggcgg ggggtgtgtc gagcttcagc ggcgccgagc ggtgtcgcgg
     4501 gtttgttgcc agcggctcag cgcttcgggt gacaggcggg tgtagcgggc gagtgaggtc
     4561 accgaggtgt ggccggagta ggcgagcagg gtggccgtgt tggcgccgtc ttcggcggcg
     4621 tgagtgagcg cgctgtgtcg tagttggtgc agtgtccatg gcccgctggg tattccggtt
     4681 gtggcatgtt cgaacagttc ggccgcgcgg cggtaggaca ggcgcgcccg gccgctgctc
     4741 ggatcgaggt cggccggtgg tagttggacc ctggcggcac ggtcggtgag gaacagcggg
     4801 ccgcttgtgc gggtgccgat caggcggggc agcagtcggg cggtgccggt ctgccagatg
     4861 acggtatcga cggcgccgcc cttgcggcgg accttggcgc ttcggttgcg ccggtcgagg
     4921 tcgtcgatgt cgagggcgag gatttcgcgg gcgcgggcgg cggtttcgta cagcatgcgc
     4981 cacagcaccc gctcgcgcag tgcgaggtcg tcgcgggtga ggagttggtc gacaagctca
     5041 cgcgcgagtc cttttgagcg gtcgggtgcc cgcccgcgcc ggcgcagcga gcgcgtgggg
     5101 tcaccgtgga tccaatcctg atcggaccag tagtgcagcg ccgaacgcag cgcgtccagg
     5161 tttcggttgt aggtcgccgc ggccttgtgc ccccaacttt gctcgaacca cccggtcagc
     5221 gtctggacct gggctggctc ggcgagcacg accagcgggg tttgtgcgtc gaaggcggtg
     5281 cgcagcgccc gcaacgttgc ggcgtagaca cgcctggttc cggccgattc gggatggtcg
     5341 agcgtcgcca ggtacgccgc gactgcctcg cccacggtcg gacccctggc cggccgcgtg
     5401 atccgatgca ccgcgctcat cgcaggcctc cgcttctatc gcagaaaagc tgccggagtc
     5461 gcaacgaact cgctatacga tctgcctgcg tgtcgcggct ccgcgccgat tccaccgcag
     5521 aaaatgtagc gttctgtgcg ataaattgtc ggcccggaac ctatcaatcg gcgcgcgatt
     5581 gggggacatc gtgcccgtga tcgtcgatcc ggccgccctc gccgggtacg ccgcctttcc
     5641 ggccgacttg gatcgggact gggtgggctc ggtgtgccac ctgagcgtcg acgacctgcg
     5701 tgcggcgggc cgccgcgtcg atgagaccac ccggttgggg tttgcggtac agctggtgac
     5761 ggtccgcgcg atcggcacct ttctggccga tcccaccgcc gtccccgatc cggtgttggc
     5821 ggcggtggcc cgccagctcg acatcgccga tccggttgtg ctgcaccgat atcgggacct
     5881 tccggtgcgg tggcggcaca ccgcggagat tcgcgaccgg tacggatatc gcgacttcac
     5941 cgctcaaccc gcgcgcttcc tgctcaccct tgatttgtat cggcaggcgt ggtccgacga
     6001 cgtcgctgcc tcggtgctgt tccgcgccgc gcaccgccga ctgctcgccg agcagatcct
     6061 gctgcccggc cacagtgtgc tggcccggct ggtgacagcg gtgcgcgatc gggccgcgcg
     6121 tcggctgcac atccgcctgg ccgctgcggc gccgcccgac ctggtcgcgc ggttgcagaa
     6181 cctgctgatc gtgccggacg gtgcgcggcg ctcggagctg gaccggctgc gtcgcccacc
     6241 gttcaccccg acgatcaccg ggctggtcaa ggccctccaa cggttggccg aggtccgcgc
     6301 gctcggcgcc ggcgcggtgg acctgtgcgg tctgcccgcc cggcgggtgg cggcgttggc
     6361 ccgctacgcc gatcaggcct gggtcaccca gctggcggat ctggccgacg aaaggcgggt
     6421 cgccacgctg ctggcctaca cccacgcgct caccgccgac gcccgcgacg acgtgatcga
     6481 catcttcgac gtggtgttcg gcgacctgca acgctccgct actcaccgcg gccagcaacg
     6541 acgggtcggg gagttgcgcg attacgaccg cgcggtcgga caactgcata cccggatgcg
     6601 atcgatcctc gacgtcctcg acgccgaccc ctactcccag accgccgtgc tcgacgggct
     6661 gcgggccgac cgggacggga ttgaagcagc gatgggtacc gtgtcggcgc tgatgcgctc
     6721 acccgccgac ccgttccacg aacggctggt cgccgcctac ccgcagatcc gtaggttcct
     6781 gccactgctg atcgaggcca tcaagctgga cgccacaaga gtccgcacga ccggtgctgg
     6841 ccgcctacca cgctctcggc gactggctta ccaacaaacc gcgcaccacc aaactccccg
     6901 acaccgagct gccgttggag gtgatcaatg ccgcctggtc gccgcacgtg cgcgacaccg
     6961 acgcgggcac cgtcaaccgg tccggctatg cctgctgtgt gctcgatgcg ttgcggaccc
     7021 ggttgcgtcg ccgcgacatc tacgcacccg cgagcacccg ttggggcgac ccccgcgccg
     7081 aactcctgac tcccgacgcg tgggaggagc agcgcgaaac cctttgtgac gagctggcat
     7141 tggacaccga cccgaccgcg ttcatcgatc aactcaccgc cgccctggac gcgtcgtggc
     7201 gggaaactgc cggcggatac gcggcgaacc ccgacctgcg tgtcgagcac cggggcgggc
     7261 gcgacgagat cgtgctgacc ccgctcgacg ccgacgacca acccgactca ctgatcgacc
     7321 tgcgtgcccg ggtggaggaa ttgttgcccg aggtggagat cgccgacctg ccgctggagg
     7381 tgcacggctg gaccggattc ctcgacgaat acacccacat ggccggcgac accggccgcg
     7441 aacccggcct gtccgaaacc ctgtcggcgc tactggtttc cgagtcctgc aacgtcggcc
     7501 tcaccccggt cgccgacgac acccaccggc cgctgactcg cgaccggttg aactgggtcg
     7561 cccacaacta cctgcgctcg gccacccacg ccgccgcgaa cacccggctc gtggactacc
     7621 acaccccgct ggcgctcgcg caggcctggg gcggcggtca gatggcctcg gcggacggca
     7681 tgcgcttcgt catcccggtc tcgacgatcc acgccgccta caacccccgt tacttcggac
     7741 gacgacgcgg ctccaccctc tactcctgga tggccgacac ccacaccgtg ttcgcgcaga
     7801 ccctgatccc cggcactcaa cgcgacagcc tttacaccct cgacgggctg ctcgccaacc
     7861 agaccctcat ccgcccggaa atggtatcca ccgaacaccg ccggcgcctc ggaaatcgtg
     7921 ttcgcgctcg cctggaccct cggctaccgg tgggcacccc ggctggcgga cctgcccgac
     7981 cagcggctct ggcacatcgg tcgacacgtc gactacgggc ccctcaccgg actggcgcgg
     8041 caccgcatca acacccggct catcatcgag aactgggacc agatctgcag gctcacagcg
     8101 tccttgaagg caggcaccgt caccgcatcc gcgatcatgc gcaccctgca gcgcggcccc
     8161 aacccctcca gcctcgcccg ggcactcgcc gaactcggca ggatcgtcaa aaccctgcac
     8221 gtactgctct actgccgcga cccggactac cgccgcacca tccgtcacct gctcaacagc
     8281 gccgaaaccc gaaacgggct ggcccgcgac gtcttccacg gtcaccgcgg acaaatccgc
     8341 aagcactacc aggtcgggca ggaaaaccag ctcgacaccc tcggcatcat gatcaacatc
     8401 atcgtgctct ggcagacgat ctacacccag gccgcgctcg accacctcga cgccaacgga
     8461 tatcaccccg accccgccga catcgcccgg ctctcaccac tcggccaccc gaccatcaac
     8521 ctcgacggcc gctaccaaac caccacccga ccaccgacga gcggcctccg acccctacga
     8581 accacaccca gatcctgaac gagcgccggg cgctcaggat cctgttccaa tcctcgtcac
     8641 aggcccatgt gcactcgaag ggagtcatcc gttccttggt gcccacgggt gatgggcgaa
     8701 cctatgaatg cgtggtgaga gtggtgaaga tcggcgtcga ctcggcgaca agctcacggc
     8761 tcgacgaact tcggcacaag tatcgccgat tcccctacga ctccaccttc aaccagctct
     8821 acaacgcaga gcgcttcgac atgtaccgag acctcggatt cgactccaca tcgcgggcca
     8881 ttcgcgccag acttgacgaa tcgccaagcg cgggaggtga tcccaccaac tca
//
LOCUS       DASWBF010000474         6479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1162556,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000474 DASWBF010000000
VERSION     DASWBF010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162556"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VF874_17285"
     CDS             <1..403
                     /locus_tag="VF874_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX9177743.1"
                     /translation="NIADYNVVTSDSVHLPPTVHLFMQATGVYVMNFLPKPAESVPGT
                     ERTPWYHRNVDYDEIAFFHGGSLYGIPMPPGLISHAPQGVHHGAPEKARERARRKFDD
                     FDSVDWKVIAIDTRRRLTPSAEVLAHDLGQH"
     gene            403..1623
                     /locus_tag="VF874_17290"
     CDS             403..1623
                     /locus_tag="VF874_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9177744.1"
                     /translation="MTTAVKHEYDRIPYLVAYQNNSGVRDVYGGVAELVVLESYLLKP
                     KTKPSAAGAAPAATSDTVLVFMHPIGGGAYLPMINALARAGHHVIYCNSRFRGIDSAL
                     LMEKVVEDLGECIKDAKNRLGYDKVVLAGWSGGGSLSVFYQQQAQHPTVTASPSGDGP
                     DLTKLGLIPADGLMLLAAHISRHGTLTEWLDASILDESDPTNRDPELDLYNPENPNQP
                     PYSEEFLARYRQAQIDRNRRITKWVRAKLAELKAAGRPDDEFAFVVHGTMADPRWLDP
                     AVDPNDRAPRSTYLGDPQWVNNAPVGLARFCTLRSWLSQWSYDDANGDAVKAGPDIAV
                     PVLVIGNLADDACTPSHTRRLFDAIGNPDKEMYEIPGANHYYSGPDQRGTLREAVGIC
                     TDWLTRHGFSLEVA"
     gene            1620..2825
                     /locus_tag="VF874_17295"
     CDS             1620..2825
                     /locus_tag="VF874_17295"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9177745.1"
                     /translation="MTVSGPLDGIRVIEVGTLISGPFAGRLLGDMGAEVIKIEPPGSP
                     DPLRTWGQAELNGHHFFWTVHARNKKAVTLNLRGAKGREVFLDLVERSDIIVENFRPG
                     TLEKWDLGYDVLRQCNPGIILVRVSGYGQTGPEAHKAGYASVAEAASGLRYMNGFPGG
                     PPPRLALSLGDSLAGMFAVQGALAALYRRTVTGEGQVVDAALTESCLAVQESTIPDYD
                     VGGVVRGPSGTRLEGIAPSNIYRTADGSWVVIAANQDTVFRRLCAAMGRPELATDDRF
                     ANHVARGRNQDELDKIIADWASEREPGEIVATLSDAGVISGPINTVAEVVEDPQLRAR
                     GMIAEHYDERVDRPVLGPGVVPVLSGTPGTIRNAGPARPGQHNDDVYIGLLGKTVDEL
                     ETLRAEGVI"
     gene            2822..3730
                     /locus_tag="VF874_17300"
     CDS             2822..3730
                     /locus_tag="VF874_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX9177746.1"
                     /translation="MTELPAHVDIREVSLRDGLQIEEPIPLRAKLELLEAVAATGVRE
                     VEATAFVSPSKVPALADAPELAAALAAFSGIEFSALVASPNGAKRALAAGLRSIEYVV
                     SASDGHSRANVGRLSAEATAQISDIVAVAHDSGASVEVIVACAWDCPFDGPTLPRRVI
                     GIVEKACDDGADRLALADTIGTTTPRRTADLFADVKAVVGGIPLGAHFHNTRGAGLAS
                     AYAAVQAGVTRLDASVGGLGGCPFAPGATGNVATEDLVYMLRDSGVEVDIDLDAAIAA
                     ARVAQRVVGHDLPSALLRAGDRMLGG"
     gene            3733..4371
                     /locus_tag="VF874_17305"
     CDS             3733..4371
                     /locus_tag="VF874_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9177747.1"
                     /translation="MPPEPQGRRHQALRVLSLKGRQTREAIEQAARKLFAERGFHGTT
                     LADITSAAGKSPAVFYRYFDDKEDLLAALAESFLHDIVEPSGVSVSLPESPDDTEFFT
                     AVVTGYWNMFKQNIGIMIAVAQLAATQQRFAHVQNEFRRFGIEIVSASVRRAQDQGYG
                     QDLNPEHIAAAIALLFENFTTVFVSSSGLGEISDHDAIVTLSTIWKKTLYGF"
     gene            4385..5629
                     /locus_tag="VF874_17310"
     CDS             4385..5629
                     /locus_tag="VF874_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177748.1"
                     /translation="MDFTLPGHLPGLLDEIDAFIEREIKPLERKNMQYFDQRREYART
                     DWENGGIPVRAWEDLLGEMRRRADAAGWLRYGLPSSLGGRDGTNVDMAVIREHLAAKG
                     LGLHNDLQDESSIVGNFPQVIMMERFGTETQRKEWSQAMIRGERSFAFGLTEPGHGSD
                     ATWLETRAERTGDGWVINGAKRFNTGVHRATNDLIFARTSGEPGQAVGITAFLVPCDA
                     PGFEVPYYWWTFNMPSDHGEVELHDVTVADDAVLGEVDRGLEVAQTFLHENRIRQAAS
                     SLGAAQYCIDRAVEYAGERVVFGKPLSVNQAVQWPLVELQTEAQMVRLLVYYAAWHLD
                     RNHHLEVSDKVSMANYRANRLVCNAADRAMQVFGGVGYTRHEPFEHIYRHHRRYRITE
                     GSEEIQIRRVAQRLFKFGRQQQ"
     gene            5724..6479
                     /locus_tag="VF874_17315"
                     /pseudo
     CDS             5724..6479
                     /locus_tag="VF874_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168318.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
BASE COUNT         1137 a         2168 c         2164 g         1010 t
ORIGIN      
        1 caacatcgcc gactacaacg tagtgacatc cgacagcgtg cacctgccgc cgaccgtgca
       61 tctgttcatg caagcgaccg gcgtgtacgt gatgaacttc ctgcccaagc ccgccgagag
      121 tgtgcccggc accgagcgca ccccgtggta ccaccgcaac gtcgactacg acgagatcgc
      181 gttcttccac ggcggttcgc tctacggtat tccgatgccg cccggactga tatcgcatgc
      241 cccgcaaggc gttcaccatg gcgcgccgga gaaggcccgg gagcgggcgc gtcggaagtt
      301 cgacgatttc gacagcgtcg actggaaggt catcgcgatc gacacccggc gccggctcac
      361 gccctccgcg gaggtgctgg ctcatgattt ggggcagcac tgatgaccac cgccgtcaaa
      421 cacgaatacg accgcatccc gtatctcgtt gcctaccaaa ataattcggg cgtccgtgat
      481 gtctacggcg gtgtcgccga gctggtggta ctggaaagtt atctgctgaa accgaagacc
      541 aagccgtccg ccgccggagc cgccccggcg gcgacatcag ataccgtgct ggtgttcatg
      601 cacccgatcg gcggtggcgc ctacctgccg atgatcaacg ccctggcacg cgccggccac
      661 cacgtcatct actgcaacag ccggttccgt ggcatcgact cggcgctgct gatggaaaaa
      721 gtggtcgaag acctcggaga atgcatcaaa gacgccaaga accggctcgg ctacgacaag
      781 gtggtgctgg ccggctggag cggcggcggc tcactgtcgg tgttctacca gcagcaggcc
      841 cagcacccga cggtgacggc cagcccgtcc ggcgacggac ccgacctgac gaagctcggg
      901 ctgatcccgg ccgacggcct catgcttctc gccgcgcaca tcagccggca cggcacgctg
      961 accgagtggc tggacgcgtc gatcctcgat gaatccgacc ccaccaaccg cgatcccgaa
     1021 ctcgacctct acaacccgga gaacccgaac cagccgccct acagcgagga gttcctggcc
     1081 cgctaccggc aggcgcagat cgaccgcaac cggcgaatca ccaagtgggt cagggccaaa
     1141 ctggccgagt tgaaggccgc cgggcgaccc gacgacgagt tcgcgttcgt cgtgcacggc
     1201 acgatggccg acccgcgttg gctggacccc gcggtcgatc cgaacgaccg cgccccgcga
     1261 agcacctacc tcggcgaccc ccaatgggtg aacaacgccc ccgtcgggct cgcccggttc
     1321 tgcacgctgc gcagctggct gtcgcagtgg agctacgacg acgccaacgg tgacgccgtc
     1381 aaagccggtc ccgacatcgc ggtgcccgta ctcgtgatcg gcaacctcgc cgacgacgcc
     1441 tgcacaccca gccacacccg ccggctgttc gacgcgatcg gcaatccgga caaagagatg
     1501 tacgaaattc ccggcgcgaa ccactactac tccggccccg atcaacgtgg gacgctgcga
     1561 gaagcggtcg gtatctgcac ggattggctg acccgccacg gcttctcact ggaggttgca
     1621 tgaccgtttc cggcccgctg gacggcattc gcgtgatcga ggtcggcacg ctgatttcgg
     1681 gcccgttcgc cggtcggctg ctcggcgaca tgggtgcgga ggtcatcaag atcgagccgc
     1741 ccggttcccc tgacccgctg cgcacctggg gacaggccga gctgaacggg catcacttct
     1801 tctggaccgt acacgcgcgc aacaagaagg cggtgacgct gaacctgcgg ggcgccaagg
     1861 gccgcgaggt gtttctggac ctcgtcgagc ggtcggacat catcgtcgag aacttccgtc
     1921 ccggcacgct ggagaagtgg gacctcggtt acgacgttct gcgccaatgc aatccaggca
     1981 tcattctggt acgtgtctcg ggttacggcc agaccggccc ggaggcgcac aaggccggct
     2041 acgcgtcggt cgccgaggcg gccagcggcc tgcggtacat gaacggattc cccggcggcc
     2101 cgccgccccg gctggcgctg tcgctgggtg acagcctggc cggcatgttc gccgtgcagg
     2161 gcgctctggc ggcgctctac cggcgcaccg tcaccggcga gggtcaggtt gttgacgccg
     2221 cactgaccga atcctgcttg gccgtccagg aatccacgat ccccgactac gatgtcggcg
     2281 gcgttgtgcg ggggccgtcg ggcacccggc tggaaggtat cgccccgtcg aacatctacc
     2341 gcaccgccga cggcagctgg gtggtgatcg ccgcgaacca ggacaccgtg ttccgtcggc
     2401 tgtgcgcggc aatggggcgc cctgaactgg ccaccgacga ccgattcgcc aatcacgttg
     2461 cgcgcgggcg taatcaagac gagctggaca agatcatcgc tgactgggca tcggagcgcg
     2521 agcccggcga gatcgtcgcg acgctgtccg acgccggcgt gatcagcggc ccgatcaaca
     2581 ccgtcgccga agtggtcgag gatccacagt tacgggcccg cgggatgatc gccgaacact
     2641 acgacgaacg agtcgaccgc cccgtgttgg gcccgggtgt cgtaccggtg ctctcgggta
     2701 cgccgggcac gatccgcaac gccggcccgg cccgaccggg ccagcacaat gacgacgtct
     2761 acatcggcct gctcggcaag accgtcgacg aactcgagac gctgcgcgcg gagggagtga
     2821 tatgaccgag ctacccgcac acgtggacat ccgcgaagtg tcactgcgtg acggactgca
     2881 gatcgaagag ccaatcccgt tgcgcgccaa gctggaacta cttgaggcgg tcgcggcgac
     2941 cggggtgcgc gaggtggagg ccaccgcgtt cgtgtcgccg tcgaaggtac ccgcgctggc
     3001 cgacgcccca gaactagcgg cagcactcgc cgccttttcg ggaattgagt tttccgcgct
     3061 ggtggccagc ccgaacggtg ccaaacgggc gctggctgcc ggacttcggt cgatcgagta
     3121 cgtagtgtcg gcatcggacg ggcacagccg ggccaatgtc ggtcgcctga gcgccgaggc
     3181 gaccgcgcag atttcggaca tcgtcgccgt cgcccacgac agcggcgcat cggtggaggt
     3241 catcgtcgcg tgcgcgtggg attgcccgtt cgacggtccc acgctgccgc gccgcgtgat
     3301 cggcattgtc gagaaggctt gcgacgacgg cgccgatcga ctggcgctgg ccgataccat
     3361 cggcacgacc acaccccgtc gcacggcgga cctgttcgcc gatgtgaagg cggtcgtcgg
     3421 cggcatcccg ctcggcgcgc atttccacaa cacccgcggc gcgggcctgg cgagcgcgta
     3481 cgccgctgtc caagcgggcg tcacgcggct cgatgcttcc gtgggtgggc tggggggatg
     3541 cccgttcgcg cccggggcaa cgggcaacgt cgccacggag gacttggtgt acatgttgcg
     3601 ggacagcggt gttgaggttg atatcgacct cgacgctgcg atcgcggccg ctcgcgtcgc
     3661 gcagcgcgtc gtgggccatg acttgccgag cgcgctactg cgggccggcg acagaatgct
     3721 cggcgggtga gcatgccgcc ggagccgcag ggccggcgac atcaggcact gcgggtgctg
     3781 agcctaaagg gccggcagac ccgcgaggcc atcgagcagg cggcccgaaa gttgttcgcc
     3841 gagcgaggct tccacggcac cacactcgcg gacatcacgt cggcggcagg caagtcgccc
     3901 gcggtgttct accgctactt cgacgacaag gaagatctgc tcgccgcgct ggccgagtcg
     3961 ttcctgcacg acatcgtcga gccgtccggc gtcagcgtct cactgccgga gtcgcccgac
     4021 gacaccgagt tcttcaccgc ggtggtcacc ggatactgga acatgttcaa acaaaacatc
     4081 gggatcatga tcgccgtcgc gcagctggct gcgacccagc agcgattcgc tcacgtgcag
     4141 aatgagtttc gccgcttcgg catcgagatc gtttccgcct cggtgcggag ggcgcaggac
     4201 cagggctacg gacaggacct gaacccggag cacatcgccg ccgcgatcgc gctgctgttc
     4261 gagaatttca cgacggtgtt cgtgtcctcg tccggtctcg gagagatcag cgatcacgat
     4321 gccatcgtga cgttgtcgac aatctggaag aaaacgctgt acggcttctg aaaaggagaa
     4381 ttccgtggat ttcacgctgc cgggtcacct tccaggactg ctcgacgaga tcgacgcgtt
     4441 catcgagcgg gagatcaagc cgctggagcg gaagaacatg cagtacttcg accagcgccg
     4501 cgagtacgcg cgcacggact gggagaacgg cgggattccg gtgcgagcgt gggaggatct
     4561 gctcggcgag atgcgaaggc gcgccgacgc ggccggctgg ctgcgctacg ggctgccgtc
     4621 gtcgctcggt ggccgtgacg gcaccaacgt cgacatggcg gtcatccgcg agcacttggc
     4681 cgccaaaggc cttggcctgc acaacgacct gcaggacgag tcgtcgatcg tcggcaactt
     4741 cccgcaggtg atcatgatgg agcgtttcgg caccgagacg cagcgcaagg agtggtcgca
     4801 ggcgatgatc cgaggcgagc gcagcttcgc gttcgggctc accgaaccgg gccacggctc
     4861 ggacgccacg tggctcgaga cccgcgccga acgcactggt gacggctggg tcatcaacgg
     4921 cgccaagcga ttcaacaccg gcgtgcaccg ggccacgaac gacttgatct tcgcccgcac
     4981 ctcaggagag ccgggtcagg cggtgggcat caccgcgttc ctggtccctt gcgacgcgcc
     5041 aggcttcgag gtgccctact actggtggac tttcaacatg ccgtccgacc acggcgaggt
     5101 ggaactgcac gatgtcacag tggccgacga cgcggtgctc ggcgaggtgg accgcggtct
     5161 ggaagttgcg cagacgttcc tgcacgagaa caggattcgt caggcggcca gcagcctcgg
     5221 cgctgcgcaa tactgcatcg accgggccgt cgagtatgcc ggcgagcggg tggtgttcgg
     5281 caagccgctg tcggtgaacc aggcggtgca gtggccgctc gtcgaactgc aaaccgaggc
     5341 gcagatggtg cggctgctgg tctactacgc ggcctggcac ctcgaccgca accaccacct
     5401 cgaggtgtcc gacaaggtgt cgatggccaa ttaccgcgcc aaccggctgg tgtgcaacgc
     5461 cgccgaccgg gcgatgcagg tgttcggcgg cgtcggctac acccgtcacg agccgttcga
     5521 gcacatctac cgccatcacc gccgataccg gatcaccgag gggtccgagg agatccagat
     5581 ccgccgggtt gctcagcggc tgttcaagtt cggccggcag cagcagtgac ggtggctggt
     5641 ccggcgcgag cgtgcacgtt tgtagccctg cggacggcgt gtcgtgcgac agacacgcac
     5701 gctcgcggca agaaggaggg gcgatgatca ccgctgacgg gctggacgag aagctcgcgg
     5761 gcgtgctgtc gccggtgctc ggcggcccgg tcaccgtcga gaacctgcac gcgctgaccg
     5821 gcggtgcgag ccgatccact tgggcattcg aggcaggcca gcgcaagctg atcctgcgca
     5881 ccggcccacc cgacgacgta cacgccggca tggaactcga ggcgcagacg cagaagctgg
     5941 ccgcagcgga agggactccg gtgccgcacg tgctgatcgc cgacgattcg gcgagccccc
     6001 tcggcgtgcc gtatttggta tgcgacttca tcgacgggga gacgatcgct cccagaattc
     6061 tccggaagct tgacgaggcc gcccgcgccc ggcttctcga actatgcgcc caggcgctcg
     6121 ccgcgatcca ccgcgtcgac cccacgaatg tcgagctgcg ccgcgacgac cagatggcgc
     6181 tctggcgcgc tcagctcgac gagatgggcg acaccaccgc cacgttcgaa tggacgctgc
     6241 gctggctggc ggccaaccgg ccgccaccgt cgccgccgtg ttggtccacg gcgacttccg
     6301 catgggcaac ctgatcatcg acgacggcca gctcgccgcg gtgctcgact gggagctcgt
     6361 gcacgtcggc gagatttacg aagacctagc ctggttctgc atccgcgcct ggaggttcgg
     6421 cgcaccgcgg agccgcagcg ccggcggatt gggcagcatc ggacaattcc tttccgcct
//
LOCUS       DASWBF010000475        10720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_57068, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000475 DASWBF010000000
VERSION     DASWBF010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10720
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57068"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VF874_17320"
     CDS             <1..529
                     /locus_tag="VF874_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HEX9177749.1"
                     /translation="GLQTNTAPVYKMPWGTVHPTTISAPIVGMAYGAYDAHVEHQGKR
                     IRAAFAGEKAKDDPFAKVRIAEAASDIDAAWRQLIGNVSDEYALLAAGKEIPFELRAR
                     ARRDQVRATGRAIASVDRLFEASGATALANSAPIQRFWRDAHAGRVHAANDPERAYLI
                     FGNNEFGLPPQDTMV"
     gene            529..1416
                     /locus_tag="VF874_17325"
     CDS             529..1416
                     /locus_tag="VF874_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9177750.1"
                     /translation="MTSFATEAENQQEVTFESTSRYAQVRDDMRLHYHEAGVANEQTV
                     VLLHGGGPGASSWSNFSRNIAVLARHFHVLAVDQPGYGYSDKHTEHEQYNRYSATALL
                     NLFDHLGVDRAPLVGNSLGGGTAARFALDNPDRAGRLVLMGPGGLSVNLFAPDPTEGV
                     KLLGKFSVEPTRENMEKFLRIMVYDQKLITPELIDERFAIASTPESLAATRAMGKSFA
                     GPDFELGMMWRDAHKLRQRVLLIWGREDRVNPLDGALVALKTIPRVQLHVFGQCGHWA
                     QLEKFDEFNRLTIDFLGGG"
     gene            1416..2321
                     /locus_tag="VF874_17330"
     CDS             1416..2321
                     /locus_tag="VF874_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9177751.1"
                     /translation="MSIRSLGYLRIAATDMAAWREFGLKVLGMVEGAGTVDGALYLRM
                     DDFPARLVIVAGEHDKLLVAGWECANASGLQEIRDRLDVEGVPYKEATQAQLADRRVD
                     EMINFQDPSGNCLEVFHGAALEHRRVVSPYGHKFVTGEQGLGHVVLSCRDDAEALHFY
                     RDVLGFKLRDSMRMPPQMVGRPANGAPAWLRFFGCNPRHHSLAFLPLPTPSGIVHLMV
                     EVENSDDVGLCLDRALRRKVPMSATLGRHVNDLMLSFYMKTPGGFDVEFGCEGRQVDD
                     HDWIARESTAVSLWGHDFTVGARNA"
     gene            2325..2891
                     /locus_tag="VF874_17335"
     CDS             2325..2891
                     /locus_tag="VF874_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9177752.1"
                     /translation="MTSAPIDPRTFRHVLGQFCTGITVITTVHEDAPVGFACQSFAAL
                     SLEPPLVLFCPTKVSRSWQAIEATGRFCVNVLHEKQKDVSARFGSKEPDKFAGIDWHQ
                     SPLGSPIIDDSLAHIDCTVASVHDGGDHFVVFGAVASLSEVPKKKPRPLLFYRGEYTG
                     IEPDKNTPAQWRDDLEAFLTATTPDTWL"
     gene            3046..3915
                     /locus_tag="VF874_17340"
     CDS             3046..3915
                     /locus_tag="VF874_17340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX9177753.1"
                     /translation="MVNPSNTWARIYLVTLTAIVGAITVAIGIWCLVDPGSFAESVGF
                     DVHEHFLHDIGAFQLGLGVTLLLALIWSDALATAIAGFIVANTVHTVNHVLDLDLGGS
                     AAQAWLLGAVSVALVAAFVVRLRQLGYVLGTVATATSPALAPFVRQKTIRLTTFRKDG
                     TPGTSPVSIVVDGERAYFRSFERAIKIRRIKRNPSVEFGSATMSGKPTGGMRPGRVRL
                     LDGAEYRTAARLLRRKYPFLHGVLVPMMHRVMRSKLGRTADAELIPTASSSGSQQGSS
                     SDAVVGQAVVPPA"
     gene            3925..4197
                     /locus_tag="VF874_17345"
     CDS             3925..4197
                     /locus_tag="VF874_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177754.1"
                     /translation="MLCYIDIDERPDAEFFEDLDDWEVGQVLKEATEAEAAAEEWWMA
                     ASEELALRRLRRSRPAPPLFERLRAAEQRRLDRRRRKRPRRRRPRT"
     gene            4450..5829
                     /locus_tag="VF874_17350"
     CDS             4450..5829
                     /locus_tag="VF874_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9177755.1"
                     /translation="MSQGNTSTASTVPTGLKRVVVASMAGTVVEWYEFFLYGTAATLV
                     FSKLFFKQGGSELDTILAAFITYAVGFAARPLGGVVFGHFGDKYGRKKLLQFSLLLVG
                     AVTVLMGCLPTFAQVGYWAPALLVVLRFLQGFAVGGEWGGAVLLVAEHSPNRSRGFWA
                     SWPQAAVPVGNMLATVALLVLTATLSEAAFLSWGWRIAFWLSAVVVLVGYYIRTKVSD
                     APIFLEAQQEAEQIKAASFGVVEVLKRYPRGVITAMGLRFAENIMYYLVVTFSITYLK
                     VQAHADTSDILWWLLIAHAVHFVAIPQVGRLSDRFGRRPVYFVGAIAAGSWGFFAFPM
                     MDSGHYFVIMSAIILGLVIHAIMYAPQPAIMAEMFPTRMRYSGVSLGYQVTAIFAGSL
                     APIIAVRLLEIYDSSLPIALYLAGAAVVTLISVLLTRETKGIDLAEVDRADTRRLAEA
                     SPVQAPPHG"
     gene            complement(5826..6941)
                     /locus_tag="VF874_17355"
     CDS             complement(5826..6941)
                     /locus_tag="VF874_17355"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9177756.1"
                     /translation="MDVWLAAAERQRTHGDLVNLSAGQPSAGAPAAVRAAAAEALEHN
                     PLGYTVALGIPELREAIAGAYQTRHGIVVDADDVVVTTGSSGGFLLAFLACFDVGDRV
                     VVTSPGYPCYRNILSALGCEVVDLPCGPETRFQPTAQMLAALDPPVRGVVVASPANPT
                     GTVIPPAELAAIATRCDDTGVQLISDEVYHGLVYPGAPATSCAWETSRNSIVMNSFSK
                     YFAMTGWRLGWMLVPPELRRAADCLTGNFTICPPVLAQFAAVAAFTPESVAESDARVE
                     HYAVNRERLLNGLRGIGLDRLAPTDGAFYVYADVSDVTTDSMLFCSKLLADTGVAIAP
                     GIDFDTVHGNSFVRLSFAGPTEDIDEALRRIEPWLQA"
     gene            complement(6983..7936)
                     /locus_tag="VF874_17360"
     CDS             complement(6983..7936)
                     /locus_tag="VF874_17360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177757.1"
                     /translation="MNDERELLRETVRGLVAKHAGPEAVRQAMDSARGYDEALWKLLC
                     EQVGAAALVVPEELGGAGGEFADAATVLSELARGLVPTPLLGTTLAELALLAADDVDA
                     DTVGQLAEGTAIGAVVFDPDYVINGDTADVVVAVDDERLTRWTTFTSNRVDTMDLTRR
                     LARVTPQDTGPLGTDPGIADAAAILLAAEQIGAAERCLELTVDYTKQRVQFGRPIGSF
                     QALKHRMADLYVAVSAARAVVDDAVAEPTSTTAAVARVAATEAFDAVAAEAIQMHGGI
                     AITWEHDIQLYFKRAHGSGQLLGPTRDHLRRLESEVLNIQA"
     gene            complement(7933..8886)
                     /locus_tag="VF874_17365"
     CDS             complement(7933..8886)
                     /locus_tag="VF874_17365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177758.1"
                     /translation="MRFALDEQQRDFAASIDAALASADLPAAVRAWASGDTAPGRKVW
                     ARLADLGVTALAVPERFGGIEAHPVDLIVALERLGRWCVPGPVTESIAVAPVLMADDD
                     RARRLASGELIATVAMPPSVPRAVDAEVAGLTLLATDGVVSDAEPQQCHASVDPSRRL
                     YDVSTSGDSRPADVARAYEVGALATAAQLVGAGQALLSAAVDYAKQRTQFGRVIGSYQ
                     AIKHKLADVHTALELARPLLYGAALSLAADSPDTARDVSAAKVAAAEAATLAARNALQ
                     THGAIGFTAEHDMSLWLLRVQALRSAWGDPAAHRRRVLEAL"
     gene            complement(8883..10016)
                     /locus_tag="VF874_17370"
     CDS             complement(8883..10016)
                     /locus_tag="VF874_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177759.1"
                     /translation="MDLDFDDATEDFRAEVGNFLAANMASFPSESYDTAEGFEQHRRW
                     DKVLFDAGLSVITWPEKYGGRDATLLQWVVFEEEYFRAGTPGRASANGTSMLAPTLFA
                     HGTEEQLDRVLPKMASGEEIWAQAWSEPESGSDLASLRSTATKTDGGWLLNGQKIWSS
                     RAVFGDRAFGLFRSDPDAERHRGLTYFMFDLRADGITVRPIAQLNGDTGFGEIFLDDV
                     FVPDEDVIGEPHDGWRAAMSTSSNERGMSLRSPARFVAAAERLAQLWKTERDSAFGDR
                     VADAWIKAQAYRLHTFGTVTRLAEGGELGAESSVTKVFWSDLDVAIHETALDIRGSDG
                     ELADAWTDGLLFALGGPIYAGTNEIQRNIIAERLLGLPREKAK"
     gene            complement(10016..>10720)
                     /locus_tag="VF874_17375"
     CDS             complement(10016..>10720)
                     /locus_tag="VF874_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9177760.1"
                     /translation="RDYDLSSLRFAVTGAAIVPVVLIERMQSELDIDIVLTAYGLTEA
                     SGFGTMCRPEDDAVTVATTCGRPIADFELRIDSPDESGAGEVLLRGPNMMLGYLDDPQ
                     ATAATIDADGWLHTGDVGTVDDAGNLRITDRLKDMYICGGFNVYPAEVEQTLMRLDGV
                     ADVAVIGVPDERLGEVGKAFVVRRTQADLDEQAVITYSREHLANFKTPRSVVFCDSLP
                     RNLAGKVVKPQLREMA"
BASE COUNT         1749 a         3559 c         3578 g         1834 t
ORIGIN      
        1 cgggctgcag accaacaccg cgccggtgta caagatgcct tggggcaccg tgcatccgac
       61 cacgatttct gcgcccatcg tcggcatggc ttacggcgct tacgacgcac acgtggagca
      121 ccagggcaag aggatccgag ccgcgttcgc cggcgaaaag gccaaggatg atccgttcgc
      181 gaaggtgcgc atcgccgagg cagccagcga catcgacgcc gcctggcgcc agctgatcgg
      241 caacgtcagc gacgaatacg cgctgctcgc cgcgggcaag gagatcccgt tcgagttgcg
      301 ggcccgggcc cgccgcgacc aagtccgcgc caccggccgc gcgatcgctt cggtcgaccg
      361 gctcttcgag gcctcgggtg cgaccgcgct ggccaacagt gctccgattc aacggttttg
      421 gcgcgacgcg cacgccggtc gggtgcacgc cgcgaacgat cctgaacgcg cgtacctgat
      481 tttcggtaac aacgagttcg gtctgccgcc ccaggacacc atggtctgat gacgtcgttc
      541 gcaaccgaag ccgagaatca acaagaggtc accttcgaat cgacctcccg gtacgcacag
      601 gtgcgagacg acatgcgcct gcactaccac gaggccgggg tagccaacga gcagaccgtg
      661 gtgctgctgc acggcggcgg gccgggcgcg tcgagttggt cgaacttctc ccgcaacatc
      721 gcggtgctgg cgcggcactt tcacgtgctc gccgtcgacc agccgggcta cggctactcg
      781 gacaagcaca ccgagcatga gcagtacaac cgctacagcg cgaccgccct gctgaacctc
      841 ttcgatcacc tcggggtcga ccgggcgccg ttggtgggca attcgctggg cggcggcacc
      901 gcggcgcggt tcgcgctgga caatccggac cgggcgggcc gcctggtgct gatgggccca
      961 ggcgggttga gcgtcaacct ctttgcgccc gaccccaccg aaggcgtcaa gctgctgggc
     1021 aagttctccg tggagccgac gcgggagaac atggagaaat tcctgcggat catggtctac
     1081 gaccagaagc tgatcacgcc ggaactgatc gacgagcgct tcgcgatcgc cagcacgccc
     1141 gagtcgctgg ctgccacccg tgcgatggga aagtcattcg ccggccccga ctttgagctc
     1201 ggcatgatgt ggcgggacgc gcacaagttg cggcagcggg tgctgctgat ctggggccgt
     1261 gaggaccggg tgaacccact cgacggggcc ctggtggcgc tgaagaccat cccgcgcgtc
     1321 cagttgcacg tgttcggcca gtgcggccac tgggcgcagc tggagaaatt cgacgaattc
     1381 aaccggctga caattgactt cctgggaggt gggtgatgag cattcgatcg ctgggctatc
     1441 tgcgcatcgc ggccaccgac atggccgcct ggcgcgaatt cggcctgaag gtactcggca
     1501 tggtcgaggg cgcgggcacc gtggacggtg cgctgtacct tcggatggac gacttccccg
     1561 cccggctggt gatcgtggcg ggcgagcatg acaagctgct ggtggccggc tgggaatgcg
     1621 cgaatgcctc tggcctacag gagatccgcg accggctcga cgtcgaaggt gtgccgtaca
     1681 aggaggccac tcaggctcag ttggccgatc ggcgcgtcga cgagatgatc aacttccagg
     1741 atccgtccgg caactgcctg gaggtgttcc acggcgcggc gctggagcac cgccgggtgg
     1801 tcagcccgta cgggcacaaa ttcgtcaccg gcgagcaggg actcggccac gtggtgctgt
     1861 cctgccgcga cgacgccgag gcgctgcact tctaccgcga cgtgctcggt ttcaagttgc
     1921 gcgattcgat gcggatgccg ccgcagatgg tggggcgtcc ggccaacggg gcgccggcgt
     1981 ggctgcggtt cttcggatgc aacccgcgcc atcacagcct ggcgtttctg ccgttgccca
     2041 cacccagcgg gatcgtgcac ttgatggtgg aggtggagaa cagcgacgac gtcgggctct
     2101 gcctggatcg cgcgctgcgg cgcaaggtgc cgatgtcggc cacgctgggc cgccacgtga
     2161 acgacctgat gctgtcgttc tatatgaaga ccccgggcgg cttcgacgtc gaattcggct
     2221 gcgaaggaag acaagtcgac gaccacgact ggatcgcccg ggaaagcacc gcggtcagtc
     2281 tgtggggcca cgacttcacg gtcggcgcgc gcaacgcctg aaccgtgacg tcagcaccca
     2341 tcgacccgcg cacgttccgc cacgtgctgg gccagttctg cacgggtatc acggtgatca
     2401 ccacggtgca cgaggacgcc ccggtcgggt tcgcgtgcca gtcgttcgcg gcgttgtcgc
     2461 tggagccgcc gctggtgttg ttctgcccga ccaaggtgtc gcgttcgtgg caggcgatcg
     2521 aggcaacggg gcggttctgc gtcaacgtgc tgcatgagaa gcagaaggac gtgtcggccc
     2581 ggttcggctc gaaggagccc gacaagttcg ccggaatcga ttggcatcag tcacctttgg
     2641 ggtcgccgat catcgacgat tcgttggcgc acatcgactg caccgtcgcg tcggtacacg
     2701 acgggggtga ccacttcgtg gtgttcggtg cggtggcgtc gctgtccgag gtgcccaaga
     2761 aaaagccgcg accgctgttg ttctatcgcg gtgagtacac cggcatcgag ccggacaaga
     2821 acaccccggc gcagtggcgc gacgacctgg aggccttcct gaccgccacc acccccgaca
     2881 cctggctcta gtcgccttcc caggacgtct ttcgtgagcg tgagccgact gcgaattttg
     2941 gcgccttgat tttcgcactg tgctcacgct cggcggtcgc cccgtcacat atccgcgcgc
     3001 tgcaccgtct cacagttaag gaagcacaag tttaagacgg gaggcattgt gaatccctcg
     3061 aatacgtggg cgcggatata cctagtcacg ctgacggcga ttgttggagc gatcaccgtt
     3121 gccatcggaa tttggtgtct ggtcgatccc ggctctttcg ccgaatccgt cggattcgat
     3181 gtccatgagc atttcctgca cgacatcggt gcgttccagc tgggccttgg cgtcacactg
     3241 ctgctcgcct tgatctggtc cgatgctttg gccaccgcca tcgcgggatt catcgtggcg
     3301 aataccgtgc atacggttaa tcacgtcctg gacctcgacc ttggtggttc ggcggcccaa
     3361 gcctggctgc tcggcgcggt ctcggtggcg ctggtggcgg cgttcgtcgt gcggctgcgc
     3421 cagctcggct acgtcctggg caccgtcgcg accgcgacca gcccggcgtt ggccccgttc
     3481 gtccgccaga agacgatcag gctcaccaca ttccgaaagg acggaactcc cggcaccagt
     3541 ccggtcagca tcgtggtcga cggagagcgc gcgtattttc ggagcttcga gcgggcaatc
     3601 aaaatacggc ggatcaagcg caacccgagc gtcgagttcg ggtcggcgac gatgtcgggc
     3661 aagccaaccg gcggcatgcg acccggccga gtacgtctgc tggacggagc cgagtatcgg
     3721 acagcagctc gcttgcttcg gcgcaagtat cccttcctgc atggcgtgct ggtgccgatg
     3781 atgcatcggg tcatgcgaag caagctcggc cgcacggcgg acgccgagtt gattcccacc
     3841 gcttcgtcaa gtgggtcaca gcaagggagt agtagtgacg ccgttgtcgg ccaagctgtc
     3901 gtacctccgg cctagcttca gttcatgttg tgctatatcg acatcgacga gcggcccgat
     3961 gccgagttct tcgaggacct cgacgattgg gaagtcggtc aggtcctcaa agaggcgacg
     4021 gaggccgagg ctgcggccga ggagtggtgg atggcggcca gcgaggagtt ggccctgcgg
     4081 cgcctgcggc gctcacggcc ggccccgccg ctgtttgagc ggttacgggc ggcggagcag
     4141 cgacgccttg accgccgaag aagaaagcgg ccacggcgac gtagaccgcg aacgtgaaac
     4201 ctttgcgacg acgcgccgcg cgggttcgca gacgtttcac actgggcgat acctaacagt
     4261 cgctatcgag cgtgaccagc gagccggacg cgcggcaggg gtcagcccgt ggtcgaaccg
     4321 atcgacggct agaagtcgac gtccgggtaa tcggcagcca gtgccgttca ttcacgccgc
     4381 cgctgtcggt tcgaagaacg gctgactgga aagcaacgca ccgagcagaa cgtgtggtga
     4441 gaatgcacta tgagccaggg caacacgtcg acagccagca cggttccaac cggactcaag
     4501 cgagtcgtcg tcgcatcgat ggccggcacg gtggtggaat ggtacgagtt cttcttgtac
     4561 gggaccgctg ccacgctggt gttcagcaag ttgttcttca aacagggcgg cagcgagctc
     4621 gacacgattc tcgcggcgtt catcacgtat gccgtcgggt tcgccgcccg tccgctcggt
     4681 ggtgtggtct tcgggcattt cggcgacaag tacgggcgca agaagctgct tcagttcagc
     4741 ctgctgctgg tgggcgccgt gacggtcctg atgggctgtc tacccacctt cgcccaagtc
     4801 gggtattggg caccagctct gctggtggtg ttgcgcttcc tgcaaggctt cgcggtgggg
     4861 ggtgagtggg gcggagcggt gctgctggtg gccgaacaca gcccgaaccg cagccgcggg
     4921 ttctgggcca gctggccgca ggccgctgtg cccgtgggca acatgctcgc cacggtggcg
     4981 ctcctggtgc tgaccgcgac gctgtcggag gcggccttcc tgtcgtgggg atggcggatc
     5041 gcgttctggt tgtccgcggt ggtggttctg gtcggctact acatccgcac caaggtcagc
     5101 gacgcgccga tcttcctcga agcgcaacag gaggccgagc agatcaaggc cgcgtcgttc
     5161 ggcgtcgtcg aggtgctgaa gcggtatccg cgcggagtga tcacggcgat gggcctgcgc
     5221 ttcgccgaga acatcatgta ctacctggtg gtcaccttct cgatcacgta tctgaaggtg
     5281 caggcccatg ccgacaccag cgacatcctg tggtggctgc tgatagctca cgcggtgcac
     5341 ttcgttgcga tcccccaagt cggccggctc tcggaccgtt tcgggcggcg cccggtgtat
     5401 ttcgtcggcg cgatcgccgc cggcagctgg ggcttcttcg cgttcccgat gatggacagc
     5461 ggccactact tcgtcatcat gagcgcaatc atcctcggtt tggtgatcca cgcgatcatg
     5521 tacgcgccgc agccggcgat catggcggag atgttcccga ccaggatgag gtattccggt
     5581 gtctctcttg gctatcaggt cactgcgatc ttcgcaggct cactcgcgcc gatcatcgcg
     5641 gtgcgactgc tcgagatcta tgattcctca ctgccgatcg cgctgtatct ggctggcgcc
     5701 gccgtcgtca cgctgatctc ggtgctgtta actcgcgaga ccaagggcat cgacctggcc
     5761 gaggtcgatc gcgccgatac ccgacgattg gccgaagcga gccccgtcca ggctccgccg
     5821 cacggctaag cctgcagcca cggctcgatc cgtcgcagcg cctcgtcgat gtcttccgtc
     5881 ggtccggcga acgacagacg cacgaacgag ttgccgtgca cggtgtcgaa atcgatgccg
     5941 ggtgcgatcg caacgccggt gtcggccaac aacttcgagc agaacaacat cgagtccgtc
     6001 gtcacgtccg agacgtcggc gtagacgtag aatgcgccgt cggtcggcgc gagccggtcg
     6061 agcccgatcc cgcgcagtcc gttcaacagc cgctctcggt tgacggcata gtgctcgacg
     6121 cgcgcgtccg actcggcgac cgattccggc gtgaacgcgg cgaccgcggc gaattgcgcg
     6181 agcaccggcg gacagatcgt gaagttgccg gtgaggcagt ccgccgcgcg gcgcaactcg
     6241 ggtggcacaa gcatccagcc cagtcgccag ccggtcatcg cgaagtattt cgagaagctg
     6301 ttcatcacaa tcgaattgcg cgatgtctcc cacgcgcagc tggtggcggg cgctccggga
     6361 tagaccagcc cgtggtacac ctcgtcgctg atcaactgca cgccggtgtc gtcgcaccgc
     6421 gtcgcgatcg ccgcgagctc ggcgggcggg atcaccgtcc cggtcgggtt ggcgggactg
     6481 gcgacgacga cgccgcgcac tggcgggtcg agcgcggcga gcatctgcgc cgtcggctgg
     6541 aatctggtct ccgggccgca cggcaggtcg acgacctcac agcccaacgc ggacaggatg
     6601 tttcggtagc acggataccc ggggctggtg accaccacac gatcgccgac gtcgaagcag
     6661 gccagaaacg cgagcaggaa gcctccggac gagcccgtgg tcaccaccac gtcgtcggcg
     6721 tccacgacga tgccatggcg ggtctgatac gcaccggcga tcgcctcccg gagttcgggg
     6781 atgcccagcg cgacggtgta gcccagtggg ttgtgttcga gggcctcggc cgcggcagcg
     6841 cgcactgcgg caggcgcgcc ggcgctgggc tgcccggccg acaggttcac caagtcgccg
     6901 tgcgtgcgct gccgttcagc cgccgccagc cagacgtcca tcacgtagaa cggcggaaca
     6961 cccgcacgaa gcgctacccg gttcacgctt gaatattcaa cacctcggac tcgagccggc
     7021 ggaggtggtc ccgcgtcggg cccagcaact ggccgctgcc gtgtgcccgc ttgaagtaca
     7081 gctggatgtc gtgctcccag gtgatcgcga tgccgccgtg catctgaatc gcttcggcgg
     7141 caaccgcgtc gaacgcctcc gtcgcggcga cgcgggccac cgcggccgtc gtcgacgtcg
     7201 gttcggcgac ggcgtcgtcg accaccgcgc gcgcagccga cacggcgacg tacaagtcgg
     7261 ccattcggtg cttgagcgcc tggaagctgc cgatcggccg gccgaactgg acccgttgct
     7321 tggtgtagtc gacagtcagt tcgaggcacc tctcggcggc gccgatctgc tcggcggcga
     7381 gcagaatcgc cgcggcgtca gcgatgccgg gatcggtgcc caatggaccg gtgtcctgcg
     7441 gcgtcacccg cgccaaccgg cgggtgagat ccatcgtgtc gacgcgattc gaggtaaacg
     7501 tcgtccaccg ggtcagccgc tcatcgtcca ccgccaccac cacgtcggcg gtgtcgccgt
     7561 tgatcacgta gtccgggtcg aacaccaccg cgccgatcgc ggtgccctca gcaagctggc
     7621 cgacggtgtc ggcgtcgacg tcgtcggcgg ccaacagcgc cagctctgcg agcgtggtac
     7681 cgagcagggg agtcggcacc aacccacggg cgagttcgga gagaaccgtt gccgcgtcgg
     7741 cgaattcgcc gcccgccccg ccgagttctt ccggcaccac caacgcggcg gcaccgacct
     7801 gctcacacaa cagcttccac agcgcctcgt cgtacccgcg tgcggagtcc atggcctgcc
     7861 gcaccgcttc cggacccgcg tgcttggcga cgagcccgcg cacggtctcg cgaagcagct
     7921 cgcgttcgtc gttcacagcg cctccagaac ccgacgacgg tgcgcggccg gatcgcccca
     7981 cgccgaccgc aacgcctgca cccgcagcag ccacaatgac atgtcgtgct ccgcggtgaa
     8041 cccgatcgca ccgtgggtct gcagcgcatt tcgtgccgcc agcgtcgcgg cttcggcggc
     8101 ggctaccttt gcggcactca cgtcgcgggc ggtgtccggc gaatccgcgg ccagcgacag
     8161 cgccgccccg tacagcagcg gccgggccag ttcgagcgcg gtgtgcacgt cggccagctt
     8221 gtgcttgatc gcctggtaag agccgatcac ccggccgaac tgcgttcgct gcttggcata
     8281 gtcgaccgct gccgacagca gcgcctgccc ggcgccgacc agctgtgctg cggtggccag
     8341 cgcgccgacc tcgtaagcgc gggcgacgtc ggccggccgg ctgtcaccgg atgtgctgac
     8401 gtcgtagagc cggcgactcg ggtcgacgga cgcgtggcac tgctgcggct ccgcgtcgct
     8461 gacgacgcca tcggtcgcca gcagcgtcaa gcccgccacc tcggcgtcga ccgctcgggg
     8521 aaccgacggt ggcatcgcaa cggtcgcgat cagctcaccg gacgccagcc gcctcgcgcg
     8581 gtcatcgtcg gccatcagaa ccggggccac cgcgatggat tccgtcaccg gcccgggcac
     8641 acaccagcgg ccaaggcgct cgagcgcgac gatgaggtcc accgggtgag cctcgatgcc
     8701 gccgaaccgc tccggcaccg caagcgcggt gacgccgagg tcggcgagcc gcgcccagac
     8761 cttccggccc ggcgcggtat caccggaagc ccacgctcgc accgccgccg gcaggtccgc
     8821 gctcgccagc gccgcgtcga tgctggccgc gaagtcccgc tgctgctcgt cgagcgcgaa
     8881 tctcatttcg ccttctcccg gggcaggccc agcaggcgct cggcgatgat gttgcgttga
     8941 atctcattgg tgcccgcata aattggcccc ccgagcgcga acaggagccc gtcggtccac
     9001 gcatcggcga gctcgccgtc ggatccgcgg atgtcgagag ccgtttcgtg gatggcgacg
     9061 tcgaggtcgg accaaaacac cttggtcacc gacgattcgg cacccagttc accaccttcg
     9121 gcgagccggg tcaccgtgcc gaacgtgtgc aggcgatagg cctgggcctt gatccacgca
     9181 tcggccacgc ggtcgccgaa cgcgctgtcg cgctccgtct tccacagttg cgccagccgt
     9241 tcggcggcgg cgacgaagcg ggctggactg cgtagggaca tgccgcgctc gttgctcgac
     9301 gtgctcatcg cggcccgcca gccgtcgtgc ggctcgccga tcacgtcctc atcgggcacg
     9361 aacacgtcgt cgaggaagat ctcgccgaag ccggtgtcgc cgttgagctg ggcgatgggc
     9421 cgcaccgtga tcccgtcggc gcgtaggtcg aacatgaaat acgtgaggcc gcggtgacgc
     9481 tctgcgtccg ggtcggagcg gaacaacccg aaggccctgt cgccgaacac ggctcgcgaa
     9541 ctccagatct tctgcccgtt cagcagccaa ccgccgtcgg tcttggtggc cgtcgaccgc
     9601 aaggaggcca ggtcgctgcc tgactcgggt tccgaccatg cctgagccca aatctcttcg
     9661 ccgctggcca ttttcgggag cacgcggtcg agctgttctt cggtgccgtg cgcgaacagg
     9721 gtcggcgcca gcatcgacgt cccgttcgcg ctggcccgtc ccggcgtgcc ggcgcggaag
     9781 tattcctcct cgaacaccac ccactgcagc agggtggcgt cgcggccgcc atacttttcc
     9841 ggccacgtga tcaccgacag gccggcgtcg aaaagcacct tgtcccaacg ccgatgctgt
     9901 tcgaagcctt ccgccgtgtc gtacgactca gacgggaacg acgccatgtt cgccgccagg
     9961 aaattcccga cctcggcccg gaagtcctcg gtggcgtcgt cgaaatccag atccattacg
    10021 ccatctctcg cagttgcggt ttgacgacct tgccagcaag gttgcgtggc agcgagtcgc
    10081 agaacaccac cgaccgtggt gtcttgaagt tcgccaaatg ctcgcggctg taggtgatca
    10141 ccgcctgttc gtccaaatcg gcctgagttc gacgcaccac gaacgccttg cctacctcac
    10201 ccaggcgttc gtcggggacc ccgatcaccg cgacgtcggc gacgccgtcg aggcgcatca
    10261 gcgtctgttc gacctcggcg ggatacacgt tgaacccgcc gcagatgtac atgtctttca
    10321 gccggtcggt gatccgtaag ttgccggcat cgtcgaccgt gccgacgtcg ccggtgtgca
    10381 gccacccgtc ggcgtcgatc gttgcggccg tggcctgcgg atcgtcgagg tagcccagca
    10441 tcatgttggg cccgcgcagc agcacctcgc ccgcgccgct ctcgtcgggc gagtcgatgc
    10501 gcagctcgaa gtccgcgatc ggtcgcccgc aggtggtcgc gacggtgacg gcgtcgtctt
    10561 ccggccggca catcgtgccg aacccactgg cctcggtcaa tccgtatgcg gtgagcacga
    10621 tgtcgatgtc gagttcggac tgcatgcgtt cgatcagcac caccggcacg atggccgccc
    10681 cggtgacggc gaaccgcagg gagctgaggt cgtagtcgcg
//
LOCUS       DASWBF010000476         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1233294,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000476 DASWBF010000000
VERSION     DASWBF010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233294"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VF874_17380"
     CDS             complement(<1..922)
                     /locus_tag="VF874_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177761.1"
                     /translation="MSGTSGEAIVAAVRRVLSVRGPMAEDDLLGVLDADGIDLGPDPD
                     VMLADVLDQDAELVMPLADERWAWIPAVLGGRIFTHRLSAVEAAHDIIVLDPDLAPLS
                     TLTECETYRRLTDGSPIAEVSAFLDGDVLAARGVPETAVDGDGVLLLRPGRFAALGVA
                     AGDLVGLRVTAHGFELVAVAQPTRCDIGAALAALLEKRPDRPEMLDVAVAVWTVCAGD
                     DNVFREAAPPLGDLLVASGLAYDGDWVARSGFDFDAWRVAGGIETIEERYQLDDDEAL
                     AVLVTVRLYEQILDVVEAVMTAQDSGEHELA"
BASE COUNT          296 a          565 c          472 g          209 t
ORIGIN      
        1 cggcgagctc gtgctcgcca ctgtcttgag cagtcatcac ggcctcgacc acatcgagga
       61 tctgctcgta gagccgcacc gtcaccagca cggccagcgc ctcatcgtcg tcgagctggt
      121 agcgctcctc gatggtctcg atcccgccag ccacccgcca cgcgtcgaaa tcaaacccgc
      181 tgcgggcgac ccagtcaccg tcgtaggcca gcccgctggc cacaagcaga tcgcccagcg
      241 gtggcgccgc ctcacgaaac acgttgtcgt ccccggcaca caccgtccac acagcaacag
      301 caacatcgag catctcgggt cgatcgggac gcttctcgag cagggcagcc agcgccgcgc
      361 cgatatcgca ccgcgtcggc tgcgcgaccg ccacgagctc gaacccgtgc gcggtgaccc
      421 gcagcccgac cagatcaccg gccgccacgc ccagcgcggc aaaccgcccg ggccgcaaca
      481 acagcacccc gtcgccgtca acagcagtct cgggcacacc gcgtgcggcc aggacgtcgc
      541 cgtcgaggaa cgcggacacc tccgcgatcg gtgagccgtc ggtgagccgc cgataggtct
      601 cgcactcggt cagcgtcgac agcggcgcca gatcgggatc caacacgatg atgtcgtggg
      661 cggcctccac cgcgctgagg cggtgcgtga agatcctccc gcccagcacg gcaggaatcc
      721 aggcccaccg ctcgtcggcc agcggcatca ccagctcggc gtcctggtca agaacgtcgg
      781 cgagcatgac gtcaggatcg ggccccaggt cgatgccgtc cgcgtcgagc acccccaaca
      841 ggtcgtcctc ggccatcgga ccgcgcaccg aaagaacccg ccgcacagca gcaacgatcg
      901 cctcaccgga agtgccagac attgcccaga cgctacccgc ccgcaccggg cgatcccgac
      961 aggacacccg ggttcaccgc gcatgcgaag cctgatcgga cggccttcgc gccgatagtc
     1021 agctccgccc gaatgattcg cagttctcgc gccagtagtg gggatgacct tggtggccgt
     1081 aggtcgaatg ctgatctacc ggcgcgacag cgcccgggcg gacaggctgc ggccgcgcag
     1141 cgcaaaacgc tgctggttct cctggtcgcc gttggagcgt tcggtacacg aggtggtgtc
     1201 tgactgaaat cgaagccgcc gcgggcgctg ccgccccgct cgcgatgcgc acagccagac
     1261 atgctcatca ctggtggagg cggaaactcc gacaccgtgc acgatgcggt tcattcggtc
     1321 gcaagccgag gtcgaggacg tggaaaacgc caacgtccga tagcgccgcc gcaacgctag
     1381 tgggaggatg gtcgcccgac agctgggcaa accgcgacag ctaaaactac tcggcgacta
     1441 attggtcgct gtcgacgaaa accagaccgc cggcgtcgag cacgaccgcc catcttcgcg
     1501 cggggtcaac tatgcgatca ccgatatcca ctgcgtgccc aa
//
LOCUS       DASWBF010000477         7188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1162750,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000477 DASWBF010000000
VERSION     DASWBF010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162750"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VF874_17385"
     CDS             <1..351
                     /locus_tag="VF874_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HEX9177762.1"
                     /translation="KLDCATLPRIEQTLGAHAAPLGLSFVDGGLPVPYAQGALVGVHG
                     SWNRQSPRAPEVSFFAWHNGTLGDQQTLVGGFQATDGSRWGRPVAALLGPDGAVYVTD
                     DYAGAVYRVAPPGR"
     gene            complement(352..639)
                     /locus_tag="VF874_17390"
     CDS             complement(352..639)
                     /locus_tag="VF874_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX9177763.1"
                     /translation="MLWTIITAIIGGLIIGALARLAMPGKQNIGIIMTIVLGVVGSFL
                     GSWICYSIFGYSNENGGWQVIPFIVGVAVAALLIFIYLAVIGRRGAGTPRT"
     gene            936..1949
                     /locus_tag="VF874_17395"
     CDS             936..1949
                     /locus_tag="VF874_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX9177764.1"
                     /translation="MLSMTPTAHADNDARQLLLAAIANTKGSYLVYNFGGEFAAPFRN
                     AAGEEYTLNNGGHLMTIKNASGRLNPRLLADTHQGYQARCERDPRARTGEGLLQASET
                     YAPLQAWQVLGQPIIAINANFFDIRSQKGGSWKQTGCSSPLGAYVDNTRGLGRTNAAV
                     TGTIAYAGKQALSGGDEVWSALATMILPIGGAPYVVMPKNDQDYDAASLEIQRLLDAG
                     TRFVAVSGIGLLAPGNTGQLNDGGPSAARTAIGYNRSNDELYVFQGGSYTPDNMQDLF
                     RGLGADNAILLDGGGSSAIVLRRDTGGMWSGAGSPRGNCDTRQVLCDSRERALPSWLA
                     FNV"
     gene            complement(1939..2721)
                     /locus_tag="VF874_17400"
     CDS             complement(1939..2721)
                     /locus_tag="VF874_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX9177765.1"
                     /translation="MTAEFVKRNPAAPQGFFACEAAGLAWLGAVDGGVPCAQVVSCDE
                     TSLTLGRINSVPPTQESARVFGVRLAVTHDAGAAAFGAAPDGWSGPGFFGPLSQPLGM
                     SLNQGRSPHTSWGRFYADERLTPMGEMAARQLSPDGRAAVAAVVKRCAAGDFDTDDGP
                     ARLHGDLWGGNLMWTADGATLVDPAAHGGHRETDLAMLALFGCPYLGDVLDGYQSVRR
                     LSPGWQRRVGLHQLYPLLAHVVLFGGSYAGQAEAAATGALTR"
     gene            complement(2718..3548)
                     /locus_tag="VF874_17405"
     CDS             complement(2718..3548)
                     /locus_tag="VF874_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9177766.1"
                     /translation="MPSVLVTGASRGIGKTIASHLAVTGWDVIAGVRTNDDSARLSAE
                     HSRITPVMLDVTNESHVAALDALLPEQLNAVVNNAAVAVGGPMETVPPAELRRQLEVN
                     VVGQIAVTQAVLPRLRASRGRIVFISSLNGRIVFPLLGPYCASKFAIEAAADALRLEL
                     KPWGIGVVVVEPAQTDTDMWRSADEVADDAFAAMPDDSRRLYDKHQVGLKKTIAMTQK
                     MAVPSEKVAAVVEEALTARRPRARYVVGFVPKLELAVLNSLPTGIRDRVVRAVAKQPR
                     "
     gene            complement(3564..3947)
                     /locus_tag="VF874_17410"
     CDS             complement(3564..3947)
                     /locus_tag="VF874_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005205762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177767.1"
                     /translation="MRLWTGDDGQSHVQVGVIAMSRPGEAGLSVLSELVPAQGISFEE
                     TPATSSLEWHTAPHRQFVITVGGRLDFVTRNGERFRLDPQTILLAEDTAGGGHRWSII
                     GTEPWQRVYVRLRANVAVPFTPDTG"
     gene            complement(3956..4672)
                     /locus_tag="VF874_17415"
     CDS             complement(3956..4672)
                     /locus_tag="VF874_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9177768.1"
                     /translation="MAQTLRVGAAFPDPPFNGAKDDGGLDISMMRAVGEVLGTTVEFV
                     PYAGADFNGIFAGLDVGDYDCVTAGTTVTPERERLATFCAPYLISGQSLAVDVSRHPQ
                     VHTVDDLAGLTIGVQQGNTSQPIAEKLVASGKAKAVRVYDYGRIRDAITDLTMGGCDA
                     FMKLAPVLTELVRRVDGIEVVQRGISTERIATAVRRSDAELLRRINAAQQELENDGRL
                     PGFRLQWLGSPVLDQSERRR"
     gene            complement(4674..5681)
                     /locus_tag="VF874_17420"
     CDS             complement(4674..5681)
                     /locus_tag="VF874_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HEX9177769.1"
                     /translation="MEDQSTDTGDLTDTADARNDAADVAAKPAPGRGVPVGKRSGRHR
                     YFRPRPVAATSEATSDASDATSDASEDADTAVAQREETSAASEITETAAAEPATVVTA
                     GGEVTPKPQRRRWFGRRSAASAVEAPTEATESAEPDAEPAEPDTEVVEFAVEEAELKR
                     APKPAGKRMAIAVVVAAVVFVAAGAFGGAMLQPYLTDRAVAETKLAIARTATDAITTL
                     FTYTPDDMDQLPARTSKYLGGDLRDAYAKQVDALAATNKQNQISRNAQVVGAAVESLN
                     GPEATAVVYANITYTSAATKDIPKIFLVSYRLTMKREGSDWVITSMPWITSKDLTRVM
                     P"
     gene            complement(5648..6166)
                     /locus_tag="VF874_17425"
     CDS             complement(5648..6166)
                     /locus_tag="VF874_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HEX9177770.1"
                     /translation="MTKRTWRDWWQGARGLPGLSIAASVLVAAAVVVMGLMLWWHVTD
                     RRTEVKNAEVLGYVGQFMTEYTTLDPFHANDYADGILANATGEFAKDFKDKQNAILIQ
                     VARAEPTKGSVLDAGIEKWHDDGSVSVLVATKISTTAGDPKQPVESGNRWVVTAVKEG
                     QQWKISRLTQVI"
     gene            complement(6223..>7188)
                     /locus_tag="VF874_17430"
     CDS             complement(6223..>7188)
                     /locus_tag="VF874_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9177771.1"
                     /translation="LHEAEPESVTEEVEASSFASWWSRAGAFAIDVLFGLTVVASFAL
                     AWFTAPFHSWLWWASTVAAGLVVLLIALNRSLAPELTGWSLGRALFGIAVVRRDGQPA
                     GFGRLLARDLAHVLDTAALFVGWLWPLWDSRRRTFADLLLRTEVRRVSGDRPEARRAV
                     GAVLTAGVVLAAATAGLTYLTVIRHEQAVDAARAQVADQGPRIVEQMLTYNAGTMKDD
                     FARALTLVTDDYREQLVQQQQVVEKNAAASNEYWAVTSSVLPGVTPDRATMLLFLQGQ
                     RQAEQQEVRFITATTRVTFEKSGDGTWRVSDLTVLAQPTLNRPGQ"
BASE COUNT         1184 a         2497 c         2312 g         1195 t
ORIGIN      
        1 aagctggatt gcgcgacgct gccacgcatc gagcagacgc tcggcgcgca cgctgcgccg
       61 ttggggctga gcttcgtcga cggcggactg cccgttccct acgcccaggg tgcgctggtg
      121 ggtgtgcacg gatcgtggaa ccgccagtcg ccgcgcgcgc ccgaggtgtc gttctttgct
      181 tggcacaacg gcactttggg cgatcagcag acactagtcg gcgggttcca ggcgaccgac
      241 gggtcgcggt ggggccggcc cgtggccgcc ttgctgggtc ccgacggcgc ggtctatgtc
      301 accgacgact acgccggcgc ggtgtaccgg gtggcgccgc cgggccggtg attatgtgcg
      361 cggtgtaccg gcgcctcgcc ttccgatgac cgcaaggtag atgaagatca gtagggcggc
      421 gacggcgact ccgacgatga aggggatcac ctgccaacca ccgttttcgt tcgagtagcc
      481 gaagatgctg tagcagatcc acgaaccgag gaacgaaccg acgacgccca gcacgatcgt
      541 catgatgatg ccgatgttct gtttgcccgg catcgcgagc cgagctagcg cgccgatgat
      601 caagccgccg atgattgcgg tgatgatggt ccacaacatg ctgctgctcc tttgtcagcg
      661 gcgccttctt gacgccgacg tcagcgggtt cccccgacgg ccgcctctta acactagacc
      721 cggcacagcg tgctggacag ggctcgggtg atttcgaaac ctgaagcggc agagatcaat
      781 tcgactggta caaaatcgtg accggaatgt tacgcaagtt gacagtgtga ttgctgtgac
      841 tatcgtggtg aaccgtccgg gaggaagacg tgccacgttc cacagcaccg ttacagcgcc
      901 tggcggcagc aaccgtcgcg gtcacgctgg gcgcgatgct gtccatgacc ccgaccgcgc
      961 acgccgacaa cgacgcccgc cagctgctgc tcgcggcgat cgcgaacacc aagggctcct
     1021 acctcgtcta caacttcggc ggtgagttcg ccgcgccgtt ccgcaacgcc gccggagagg
     1081 agtacacgct caacaacggc gggcacctga tgacgatcaa gaacgcctcg ggccggctga
     1141 acccgcggct gctcgccgac acgcatcagg gctatcaggc ccgctgcgaa cgcgatccgc
     1201 gggcgcgcac cggcgaggga ctgttgcagg cctccgaaac ctatgcgccg ctgcaggcat
     1261 ggcaggtgct cgggcagccg atcatcgcga tcaacgcgaa cttcttcgac atacgttctc
     1321 agaagggcgg gtcgtggaag cagaccggtt gcagctcccc gctgggcgcc tacgtcgaca
     1381 acacccgggg cctgggccgc accaacgcag cggtcaccgg caccatcgcc tacgccggca
     1441 agcaggcgct gtccggcggc gacgaagtct ggtcagcgtt ggccacgatg atcctcccga
     1501 tcggcggcgc cccgtacgtc gtgatgccga agaacgacca ggactacgac gccgcgtccc
     1561 tggagatcca acgactgctg gacgcgggca cgcggttcgt ggcggtgagc ggcatcggtc
     1621 tgctggcccc cggcaacact ggccaactca acgacggcgg cccgagtgcg gcccgcaccg
     1681 ccatcggcta caaccggtcc aacgacgaac tgtatgtgtt ccaaggcggc agctacacgc
     1741 ctgacaacat gcaggacctg ttccgcggcc tcggcgccga caacgcgatt ctgctcgacg
     1801 gcggcgggtc gtcggcgatc gtgctgcgtc gcgacaccgg cggaatgtgg tcgggcgcgg
     1861 gatccccgag aggcaactgt gatacccggc aggtgctgtg cgactcgcgg gagcgcgcac
     1921 tgccaagctg gctggccttc aacgtgtgag cgcgccggtg gccgcggctt cggcttggcc
     1981 cgcgtagctg cctccgaaga gcacgacgtg ggccagcagc ggatagagct ggtgcaaccc
     2041 gactcgacgc tgccatcccg gcgacaacct gcgcacggac tgatagccgt cgaggacgtc
     2101 gcccagatac gggcagccga acagcgccag catcgccagg tcggtttcgc ggtgaccgcc
     2161 atgggcggcc gggtcgacaa gcgtcgcgcc gtcggccgtc cacatcaggt tgccgcccca
     2221 caggtcgccg tgcagccgtg ccggtccgtc gtctgtgtcg aagtcgcctg cagcacaacg
     2281 cttcacgacg gcagccaccg cggcccggcc gtcaggcgat agctgccgcg cagccatctc
     2341 gcccatcggt gtcaaccgct cgtccgcgta gaaccggccc cacgaggtgt gcggcgacct
     2401 gccttggttt agggacatcc ccagcggttg cgacagcggg ccgaaaaagc ccggcccact
     2461 ccagccgtcg ggcgcggcac cgaacgccgc cgcgccggcg tcgtgggtga ccgcaagccg
     2521 cacgccgaac acgcgtgccg attcctgcgt cggcggcacc gagttgattc ggcctagcgt
     2581 caggctggtc tcgtcgcacg agaccacctg cgcgcacggc acacccccgt ccactgcgcc
     2641 gagccacgcc aatccagcgg cctcgcaggc gaaaaaccct tgcggcgcag caggattgcg
     2701 cttgacgaac tccgcggtca ccggggctgc ttcgccaccg cgcgcaccac gcggtcgcga
     2761 atgcccgtcg gcaagctgtt gagaaccgcc aactccagct tggggacgaa cccgacgaca
     2821 tagcgcgcac ggggacgacg cgcggtgagc gcctcctcga cgaccgccgc gaccttctcc
     2881 gacggtaccg ccatcttttg cgtcatcgcg atcgtcttct tcagccccac ctggtgtttg
     2941 tcgtagagcc gccgtgagtc gtcaggcatc gccgcaaacg catcgtccgc cacttcgtcg
     3001 gcgctccgcc acatatcggt gtcggtctga gccggttcga cgaccaccac gccgatgccc
     3061 cacggcttga gttcgagccg caacgcatcc gcggcggcct cgatcgcaaa cttcgacgcg
     3121 cagtacggac cgagcagcgg gaacacgatt ctgccgttga ggctggagat gaacacgatt
     3181 ctgccgcggg aggcacgcag acgcggcaac accgcctgtg tcaccgcgat ctggccgacc
     3241 acgttgacct ccagttgccg gcgcagctcg gccggcggca cagtctccat cggcccgccg
     3301 accgcgacag cggcgttgtt caccaccgcg ttgagctgtt caggcaacaa ggcgtcgagt
     3361 gcggcaacgt gactctcgtt ggtcacgtcg agcatgaccg gggtgatgcg ggagtgttca
     3421 gcgctcagtc gagcgctgtc gtcattcgta cggacgccgg cgatgacgtc ccagccggtg
     3481 acggcgagat ggctggcgat ggtcttaccg atgcctcgag aggcgccggt gacgagaact
     3541 gaaggcatga aatcacccta tggtcatccg gtgtccgggg tgaacggcac ggccacattg
     3601 gcgcgcagac gcacatagac ccgctgccac ggctcggtgc cgataatcga ccagcgatgg
     3661 cctccccctg cggtgtcctc ggcgagcagg atcgtctgcg ggtcgagccg gaagcgctcg
     3721 ccgttgcggg tgacgaagtc gagccggccg ccgacggtga tgacgaactg gcggtgcggc
     3781 gccgtgtgcc actctagtga gctggtcgcc ggtgtctcct cgaagctgat cccttgggcg
     3841 ggaacaagtt ccgacagcac cgacagccca gcctcgccgg gtcggctcat cgcgatcacg
     3901 ccgacctgca catgcgactg accgtcgtcg ccggtccaca atcgcacaca gcggatcatc
     3961 ggcgccgctc gctctggtcg aggactggcg agcccagcca ctgcaacctg aatccgggta
     4021 gtcgaccgtc gttctctagc tcctgttgcg cggcgttgat gcgccgcagc agttcggcgt
     4081 cggatcggcg caccgcggtg gcgatccgct cggtcgaaat gccgcgctgc accacctcga
     4141 taccgtcgac gcgacgcacc agctccgtca ataccggcgc aagcttcatg aacgcgtcac
     4201 aaccccccat tgtcaggtcg gtgatcgcgt ccctgatgcg gccgtagtcg tagaccctca
     4261 ccgccttcgc ttttcccgaa gcgacaagct tttcggcgat cggttggctc gtattgcctt
     4321 gttgcacacc gatggtcagg ccggcaaggt cgtcgaccgt gtgcacttgt gggtgccggg
     4381 acacgtccac ggccagcgac tgcccggaga tcaggtacgg cgcgcaaaac gttgcgagcc
     4441 gctcccgctc gggagtgacc gtggttccag cggtcacgca gtcgtagtct cccacgtcga
     4501 ggcccgcgaa gattccgttg aaatctgcgc ccgcgtacgg cacgaattcg acggtcgtgc
     4561 ccagcacctc gccgaccgct cgcatcatcg agatatccag cccgccatcg tctttcgccc
     4621 cgttgaacgg cggatcggga aaagcggctc caacacggag agtctgtgcc atgtcagggc
     4681 atgacgcgcg tcaagtcctt cgaggttatc cacggcatgc tggtgatcac ccaatccgag
     4741 ccttcgcgtt tcatcgtcaa ccggtaggag accagaaaaa tcttcgggat gtccttggtg
     4801 gcggcgctcg tgtaagtgat gttggcatac accacggccg tcgcttcggg cccattcagc
     4861 gattcgacgg cggcaccgac gacctgggcg ttgcggctga tctggttctg cttgttggtg
     4921 gcggccagtg cgtcgacctg cttggcgtac gcgtccctca ggtcgccgcc gaggtatttc
     4981 gacgtccgcg ccggcagctg gtccatgtcg tcaggcgtgt acgtgaagag tgtggtgatg
     5041 gcatccgtgg cggtgcgcgc gatggccagc ttggtttcgg ccaccgcgcg gtccgtgagg
     5101 tacggctgca gcatcgcgcc gccaaatgcg cccgcggcca cgaacaccac cgccgccacc
     5161 accaccgcga tggccatcct cttgcctgcg ggcttaggcg cgcgcttgag ttcggcctcc
     5221 tcgacggcaa actccacgac ttctgtgtcc ggctccgcag gctctgcgtc aggctcggca
     5281 gactccgtgg cctcggtcgg cgcctcgact gccgacgcgg ccgaccggcg gccaaaccat
     5341 ctgcggcgct gaggttttgg tgtgacctcc ccgcctgcgg tgacgacggt ggcgggctct
     5401 gcggctgcgg tttcggtgat ctcgctcgca gcggacgtct cttctcgctg cgcaacggca
     5461 gtgtcggcgt cctccgatgc atccgacgtc gcatccgacg catccgacgt cgcctccgac
     5521 gtagccgcga cgggccgcgg ccggaagtag cgatgcctgc cgctgcgctt gcccaccggc
     5581 acgccgcgcc caggcgcggg ctttgcggcg acgtctgccg cgtcgttccg cgcgtcggcg
     5641 gtgtccgtca gatcacctgt gtcagtcgac tgatcttcca ctgctgccct tccttgacgg
     5701 cggtcaccac ccaccggttg ccgctctcga ccggttgctt cgggtccccg gcggtggtgg
     5761 agatcttcgt cgccaccagt acgctcacgc tgccgtcgtc gtgccacttc tcgatcccag
     5821 cgtccagcac cgatcccttc gtgggttcgg cgcgcgcgac ctggatcagg attgcgttct
     5881 gcttgtcctt gaaatccttg gcgaattcgc cggtggcatt cgcgaggatt ccgtcggcgt
     5941 agtcgttggc gtggaacggg tccaaggtgg tgtattcggt catgaactgg ccgacgtagc
     6001 cgagcacctc ggcgtttttc acctcggtcc tgcggtcggt cacgtgccac cacaacatca
     6061 gacccatcac caccaccgcc gcggcgacaa gcaccgacgc ggcgatgctc aggcccggca
     6121 ggcctcgcgc gccctgccac cagtcccgcc aggtgcgctt ggtcaccgga gcgacgacgc
     6181 actcgtcgcc gctactgccg ggttcgatcc tgcgccgcgg actcactgcc ccggcctgtt
     6241 caacgtgggc tgcgccagca ccgtcaggtc ggaaacccgc cacgtgccgt cgcccgattt
     6301 ctcgaacgtg acgcgcgtgg tcgcggtgat gaaccggacc tcctgctgct cggcctgccg
     6361 ctgcccctgc aagaacagca gcattgtcgc gcggtcgggg gtaactcccg gcaacaccga
     6421 gctcgtcacc gcccaatact cgttgctcgc ggccgcgttc ttctccacga cctgttgctg
     6481 ctgcacgagt tgctcgcggt agtcgtcggt gacgagagtc agcgcccgcg cgaagtcgtc
     6541 cttcatggtg ccggcgttgt aggtcagcat ctgctcgacg atccgcggac cctgatcggc
     6601 gacctgcgcg cgggccgcgt cgacggcttg ctcgtggcga ataaccgtca agtaggtcag
     6661 cccggcagtc gccgcagcaa gcaccacccc ggccgtcagc accgcgccga cggcacgacg
     6721 ggcctctggc cggtcaccgg agacccgacg gacctcggtg cgaagcagca ggtcggcgaa
     6781 cgtgcgtcga cgcgagtccc acagcggcca cagccacccg acgaacagcg cggcggtgtc
     6841 caagacgtgc gccagatcgc gcgccagaag tcgcccaaaa cctgcaggct ggccgtcacg
     6901 ccgcacgacg gcgatgccga acaacgcccg gcccaggctc catcccgtga gttcgggcgc
     6961 gagcgagcga ttcagtgcaa tcagcagaac gaccaggccg gcggccacgg tcgacgccca
     7021 ccacagccag ctgtggaacg gtgccgtgaa ccacgccaac gcgaacgacg ccaccaccgt
     7081 caggccgaac agcacatcga tcgcaaacgc cccggcccgc gaccaccacg acgcgaacga
     7141 actggcctct acctcttcgg tgacagactc cggttcggcc tcgtgtag
//
LOCUS       DASWBF010000478         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_709551,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000478 DASWBF010000000
VERSION     DASWBF010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709551"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VF874_17435"
     CDS             complement(<1..164)
                     /locus_tag="VF874_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX9177772.1"
                     /translation="MTDTQVTWLTQEAYDRLKAELDQLIANRPVIAAEINDRREEGDL
                     RENGGYHAAR"
     gene            complement(387..809)
                     /locus_tag="VF874_17440"
     CDS             complement(387..809)
                     /locus_tag="VF874_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HEX9177773.1"
                     /translation="MTERPPARYGRQPKSRLSRRWIIVGLTVVILAIGVAVALVGYQR
                     LGRSDVSGTLGGYKLVDDETVEVTITVNREDPSRPAVCIVRGRSRDGSETGRREVLVP
                     PSTQDSVQVTTIVKTSRPPVVGDVYGCGFDVPDYLVAP"
     gene            887..1753
                     /gene="mca"
                     /locus_tag="VF874_17445"
     CDS             887..1753
                     /gene="mca"
                     /locus_tag="VF874_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HEX9177774.1"
                     /translation="MSEYRLLAVHAHPDDESSKGAATTARYATEGHRVLVVTLTGGER
                     GSILNPAMDLPEVHGRIHEIRRDEMAKAAEILGVEHTWLGFEDSGLPEGDPPPPLPDG
                     CFALVPLEEPAEALVRVIREFKPHVMTTYDENGGYPHPDHIRCHQVSVAAFEAAGDPD
                     RFADAGDPWHVHKLYYNHGFLRQRMQLLQDEFAKHDQQGPFEKWLKHWDPEHDVFAHR
                     VTTRIECWEYFSQRDDALRAHATQIDPNSDFFAAPIEWQQRLWPTEEFELARSRVPVN
                     LPETDLFTGIDP"
     gene            1750..2025
                     /locus_tag="VF874_17450"
     CDS             1750..2025
                     /locus_tag="VF874_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177775.1"
                     /translation="MTTLITLLADDAPRNTGPDFGKASPVGLLIIVLLLIGTFALVWS
                     MNRHLKKLPDSFDRENPEPDQTADDGTVALPAEPTPDDEDSRREPGG"
     gene            2009..4003
                     /locus_tag="VF874_17455"
     CDS             2009..4003
                     /locus_tag="VF874_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9177776.1"
                     /translation="MSPAANSLAQATSPYLRQHADNPVHWQQWTAEALAEAASRDVPI
                     LLSIGYAACHWCHVMAHESFEDDGVAAAMNAEFVCVKVDREERPDLDAVYMNATVALT
                     GHGGWPMTCFLTPDGRPFFCGTYYPKPQFLQLLAAVTNTWRTRRDDVERAGDQIASEL
                     RRLSSGLPAGGPAVASSLCDEAVAAVLADEDVERGGFGGAPKFPPSALLEALLRNYER
                     TGDPAPLATVQRTATAMARGGIYDQLAGGFARYSVDASWIVPHFEKMLYDNALLLRAY
                     AHWARRTGDPLARRVAAETAAFMLDALGVGGMFASSLDADAAGREGSTYVWTPDDLRD
                     VLGDDDGHWAATLFAVTQAGTFEHGSSVLQLPDDPDDPERFARVRDALMAARRDRVQP
                     SRDYKVVTAWNGLAVTALAEAGVALGRPDLVDAALRCGDSLLILHLVNGRLRRASLGG
                     EVGDSPAILEDYGALATGLLTLHQVTGEASWLMAATDLLDTALAQFADPDSPGRWFDT
                     AADAERLVLRPSDPADGATPSGAALITEALLIAGYLAPEGDRYRQAAGQALTAHTLLI
                     ARAPRSAGHWLAVAEAAVRGPLQIAVACDPADSRLLTDARLLAPGGAIVVGGAPDSSE
                     LLAGRGRVGGSDAAYVCRGPVCDLPVATGGELAAALGAPV"
     gene            4014..4384
                     /locus_tag="VF874_17460"
                     /pseudo
     CDS             4014..4384
                     /locus_tag="VF874_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
BASE COUNT          719 a         1612 c         1572 g          751 t
ORIGIN      
        1 tcgcgggcgg cgtggtagcc gccgttctcg cgcaggtcgc cctcctcgcg ccggtcgttg
       61 atctcggcgg cgatgaccgg gcggttggca atcagctggt cgagctccgc cttgaggcgg
      121 tcgtatgcct cctgggtcaa ccaggtcacc tgggtgtcgg tcatctcgtc gcgctcctca
      181 ttcctcgtgt cacgatcgct cgcgacggca gttcaacgcc tcactgccgg cagcatgcgg
      241 acacaccgcg tgtattgccg ttttttcgga ctggtaacca gttcaaatgc agcaatacac
      301 ggccccagca ggaaccgtgt atttaccaag cctaccaccg cgaggtcatt cgccattcag
      361 ggtttcgcaa tttgcgatca gccgcctcac ggcgcgacca ggtagtccgg cacgtcgaag
      421 ccgcagccgt acacgtcgcc caccaccggc gggcggctgg tcttgacgat cgtcgtcacc
      481 tgcaccgagt cctgcgtcga cggcggcacg agcacttctc ggcgaccggt ttcgctaccg
      541 tctctggacc ggccgcggac aatgcacacg gccggccggg acggatcctc gcggttgacc
      601 gtgatcgtca cttccacggt ctcgtcgtcg acgagcttgt accccccgag cgtgcccgag
      661 acgtcgctgc ggcccagccg ttggtagccg acgagcgcca ccgcgacgcc gatcgcaaga
      721 atgactaccg tcagtccgac gatgatccag cgacgggaaa gacgcgactt cggctgccgc
      781 ccgtagcggg cgggcggacg ctcggtcatg tttggatgcc cgtcggtcga ggggactcgc
      841 taggactgga actataggga ccgtcaccac agcacagagg acagacgtga gcgagtaccg
      901 gttgttggcc gtgcatgcgc acccggacga cgagtccagc aagggcgcgg cgacgacggc
      961 gcgctacgcc acggaaggcc accgcgtgct ggtggtgacg ctgaccggcg gcgagcgcgg
     1021 cagcattctc aaccccgcga tggatctgcc cgaggtgcac ggccggatcc acgagatccg
     1081 gcgtgacgag atggccaagg ccgccgagat cctcggcgtc gagcacacct ggctcggctt
     1141 cgaggactcg gggctgcccg agggcgatcc acctccgccg ctgcccgacg gctgcttcgc
     1201 gctcgtgccc ctcgaggagc ctgccgaggc gctcgtgcgc gtcatccgcg aattcaagcc
     1261 acacgtgatg accacctacg acgagaacgg cggctacccg cacccggacc acattcgctg
     1321 ccaccaagtt tcggtcgccg cgttcgaagc cgcgggcgac ccggacaggt tcgcggacgc
     1381 cggcgacccc tggcacgtgc acaagctgta ctacaaccac ggctttttgc gtcagcgcat
     1441 gcaactgctt caggacgagt tcgccaagca cgaccagcaa ggtccgttcg agaagtggct
     1501 caagcactgg gaccccgagc acgacgtgtt cgcccatcgc gtcaccacgc gcatcgaatg
     1561 ctgggagtac ttcagtcagc gtgacgacgc gcttcgtgca cacgccacgc agatcgaccc
     1621 caacagcgac ttcttcgccg cgccgatcga atggcagcaa cggctttggc ccacagagga
     1681 attcgagctc gctcggtccc gggtgccggt gaatctgccg gagaccgacc tgttcaccgg
     1741 gatcgaccca tgacgacgct catcacgctg cttgccgacg acgcgccgcg caacaccggc
     1801 cccgacttcg gcaaggccag cccggtgggg ctgctgatca tcgtgctgct gttgatcggc
     1861 accttcgcgc tggtctggtc gatgaacagg cacctcaaga agcttcccga cagcttcgac
     1921 cgcgagaacc cagaacccga ccagaccgcc gacgacggca ccgtcgcgct gcccgccgag
     1981 ccgactcccg acgacgagga ttcccgccgt gagcccggcg gctaacagcc tcgcccaggc
     2041 caccagcccg tatctgcgcc agcacgccga caacccggtg cactggcagc agtggacggc
     2101 cgaggcgctg gccgaggccg cgtcgcggga cgtccccatc ctgctgtcga tcggatacgc
     2161 cgcctgtcac tggtgtcatg tgatggctca tgagtcgttc gaagacgacg gagtggcggc
     2221 cgcgatgaac gccgagttcg tctgcgtgaa ggtcgaccgt gaggagcggc cagacctcga
     2281 cgcggtttac atgaacgcga ccgtcgcgtt gaccggtcac ggcggctggc cgatgacctg
     2341 tttcctcact cccgacggcc gtccgttctt ctgcggcacc tactacccga aaccgcagtt
     2401 ccttcaactg ctcgcggcgg tcaccaacac gtggcgcacc cgccgcgacg acgtcgagcg
     2461 agccggcgac cagatcgcct cggagctgcg caggctgtcc tccggattgc cggccggcgg
     2521 cccggcggtc gcgtcgtcgc tgtgtgatga ggccgtcgcg gcggtcctgg ccgacgagga
     2581 cgtcgagcgc ggcgggttcg gtggcgcacc gaagttcccg ccgtcagcgc tgctggaggc
     2641 cctgctgcgc aactacgagc gcaccggcga tccggcgcct ctagccaccg tgcagcgcac
     2701 cgcgaccgcg atggcgcgtg gcgggatcta cgaccagctc gccggcggct tcgcccgcta
     2761 cagcgtggac gcgtcgtgga tcgtcccgca cttcgagaag atgttgtacg acaacgcgct
     2821 actgctgcgc gcctacgcgc actgggcgcg tcgcaccggc gacccgttgg cccggcgggt
     2881 tgccgccgag accgcggcgt tcatgctcga cgcgttgggg gtgggcggga tgttcgcgtc
     2941 ctcgttggac gccgacgccg ccggccggga aggctccacc tatgtttgga cccccgacga
     3001 cttgcgcgac gttctcggtg acgacgacgg gcattgggcc gcaacgcttt tcgctgtcac
     3061 tcaggccgga acgttcgagc acggcagctc ggtgctgcaa ctaccggacg accccgacga
     3121 ccccgaacgg tttgcccgcg tccgcgacgc gctgatggcc gcacgccgcg accgcgtgca
     3181 gccaagccgt gactacaagg tcgtcaccgc gtggaacggg ctggctgtga ccgcgctggc
     3241 ggaggccggc gtggcgctgg gccggccgga tctggtcgac gccgcactgc gctgcggtga
     3301 ctcgctgctg atccttcatc tggtcaacgg ccggctgagg cgcgccagcc tcggcgggga
     3361 ggtcggggac agtcccgcga tcctcgagga ctacggcgcg ctggccaccg gcttgttgac
     3421 gctgcatcag gtcaccggtg aggcgtcctg gctgatggcc gcaaccgacc tgctggacac
     3481 cgcgctggcg cagttcgccg acccggacag tcccggccgg tggttcgaca ccgcggccga
     3541 tgccgagcgg ctggtgctgc ggccgtccga cccggcggac ggcgcgaccc cgtccggtgc
     3601 ggcgctgatc actgaggcgc tgctgattgc cggctacctt gcccctgagg gcgaccgcta
     3661 ccgtcaggcg gccggacagg cgctgaccgc gcacacgctg ctgatcgcgc gcgccccgag
     3721 gtctgccggc cactggctgg ccgtcgccga ggccgcggtg cgcggaccgc tgcagatcgc
     3781 ggtggcgtgt gatcctgcgg actctcggct gctcaccgat gcgcgcctgc tcgcgccagg
     3841 tggggcaatc gtggtcggtg gcgcgcccga ctcgtcggag ctgctcgccg gccgcggccg
     3901 ggttggcggg tccgacgcgg cctacgtctg tcgcggtccg gtgtgcgacc tgccggtagc
     3961 caccggcggg gagctcgcgg cggcgttggg cgcgcccgtg tagcgtgccg ctcatgtcga
     4021 gcgccgacga caacacgatg gccgtgaagc gctacctcga gctggtcgcc aagggcagcg
     4081 ccgacgacat caccgcgctg ttcgccgacg acgccaccat cgaggacccg atcgggtccg
     4141 atctgcgccg cggtcgcgac gcgatccacg ggttctacgc ggcgttcgaa aacgcggaaa
     4201 aggcaacgga attggtggcg atcaaggccg ccggcagcga agtcgcgttc ctgtggcgtc
     4261 tgacgatcag caccggggac accggaaacc ggatcgagat aatcagtgtg atgacgttcg
     4321 acgacgacgc gaagatccct cgatgcgcgc gttctggtca cccgccgacc tcacccccct
     4381 ctagcgcgag cgtgcgggtc tgtaccccca ggactctcga catagaggcg gctcgctgct
     4441 gacgagattg gcgtttctgc tgatagtcgc ctgtttcggg gtgtgtcgtt ggcgggattc
     4501 gacgctgctc tgtgaggtca tttgtctgac agttcggctc ggatcgaggt ggtccacggt
     4561 ggctgtggtg acccagccgg tgctgccgct gactcctgca gaggcgacgc cgatcggccc
     4621 gatcgcggcg ctggtggaca agccgggcga gggt
//
LOCUS       DASWBF010000479         5796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_709616,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000479 DASWBF010000000
VERSION     DASWBF010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709616"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VF874_17465"
     CDS             <1..219
                     /locus_tag="VF874_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9177777.1"
                     /translation="FLAQLEQDGITPPSAKQAIKDAKAVCSMLDDGYSADEVIDAVAE
                     STGLDADGANTFAVDAASAYCPEYVTES"
     gene            complement(301..1065)
                     /locus_tag="VF874_17470"
     CDS             complement(301..1065)
                     /locus_tag="VF874_17470"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017205063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HEX9177778.1"
                     /translation="MRWDPTQYGRFAAERDRPFFDLTARIEASTPRHVVDVGCGQGHL
                     TATLAERWPDAIVEGLDSSPEMIGDAADVAGVTFSVADAGDWQPTCDVDVIVSNAALQ
                     WVPRHQSLMTRWASALPSGGWLAVQVPGNFDSSSHVLMRRLAGESRWEAKLRGVVHHT
                     DAVGTPESYAATLLDAGLRADVWETTYLHVLTGDDPVLEWIRGTGLRPILAALSPDDA
                     AEFAAQLAVELREAYPPGPHGTVFPFRRVFAVGHRP"
     gene            1143..1499
                     /locus_tag="VF874_17475"
     CDS             1143..1499
                     /locus_tag="VF874_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177779.1"
                     /translation="MHRRLVPLAVLAAVVFAVVPACSTKKAPEESPTPSTTSAPPQSH
                     GVYESCLTQHGVPSPPAGPPPGPEPVPAGPLPGPVEGTTSANPAPPPPGVDQSTWDNA
                     LKACASLQPTTPTSTR"
     gene            1519..2715
                     /locus_tag="VF874_17480"
     CDS             1519..2715
                     /locus_tag="VF874_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9177780.1"
                     /translation="MTSSADPVDPPLPVPNVPGADAGARGLPDRSELSLGHRLIVDAS
                     AVADIGLRTFVASLVGAAMLPPIAAELVRRSDTGRERDNMRFYAELAAERNPVASFPA
                     PQQPPRIFSRPANPVAEWLAHGRVHNIQFDSSFEPVNPAMRDRWRRLERNNVVRAQHW
                     RHEDGPHPTLCLIHGFMGSPYLLNGLWMSLPWFYRSGYDVLLYTLPFHGRRAEKYSPF
                     SGYGYFANGLTGFAEAMAQAVHDFRSLIDYLQFAGVEKIALTGISLGGYTTALIAAVE
                     DRLAAVIPNVPVVSIEDELDDWFPASKLFTLGRKLSRIDREEFIAASAYHSPLTYPPL
                     VPRDRRLIITGLGDRLAPPEQAEMLWHHWDHCHLHWFPGNHILHVSQPEYLRRMTRFL
                     RDFMFD"
     gene            2766..3482
                     /locus_tag="VF874_17485"
     CDS             2766..3482
                     /locus_tag="VF874_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007319093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX9177781.1"
                     /translation="MPGLELLVRVGLATLLGIAIGMERQWRARMAGLQTMALVSMGAA
                     LFVILGAYAFKGDSDPTRVAAQVVTGIGFLGAGVIMKQGSSVTGLNTAATLWATAAVG
                     SLAGAWMWREAVVGAGIILAGNTFLYPLAHRMDRARSRAGREIPPTDYVFEVECATEA
                     NARVKALLLDALSQTDLHLTSLRSAGTTSPDEIELRAELFAPARHDELLESAVRAVSG
                     DPDVRSVRWSVLNEAGVRWS"
     gene            complement(3484..4380)
                     /locus_tag="VF874_17490"
     CDS             complement(3484..4380)
                     /locus_tag="VF874_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9177782.1"
                     /translation="MRTDDDTWDITTSVGSTALFVAAARALHAQCDEPLAVDPYAEVF
                     CRAVGGLWADVLDSKSPDHKLRSEFGEVFQTYQGARTRFFDDFFRNAAAEGVSQIVIP
                     AAGLDSRAYRLPWPDSTVVFELDQPKVLEFKRTVLADHGDLPTAKRREVAADLREDWP
                     KALRDSGFEPAQPSAWLAEGVLMYLPATAQEQLYAGIDSLAAPGSRAALEEMHPLDEE
                     DLEAKRAEERAAGNQPGTFFTLIYNEKHRDAVKWFGQHGWTVEGIVTADYFRQIRQPV
                     PPPDTDGGQMLRTGSLVTAAKR"
     gene            4588..5526
                     /locus_tag="VF874_17495"
     CDS             4588..5526
                     /locus_tag="VF874_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9177783.1"
                     /translation="MSDIRFARLHSDRVAYRDEGDLAAEPLVLIHGMAGSSLAWVPVI
                     GPLSAKYRVIAPDLLGHGQSDKPRTDYSLGAFAVWLRDFLDELDVPSATLIGHSLGGG
                     VALQFAYQHRQYCRRLVLVNSGGLGTDISMPLRLLSTPGAELLLPVLASRPAMFVGNG
                     VLSLLAARGVESPKVQDRWRKYSALADPLVRQAFLRTLRSVVDLRGQTVCALDRLPRL
                     VGDLPTLIVAGARDAVIPADHAEAAHAVLPGSRLEIIDDAGHHPQLDCPEALAQLIDE
                     FVSSDDRALSRRGAAGAATSGSGRARLWHRPTPRGA"
     gene            complement(5578..>5796)
                     /locus_tag="VF874_17500"
     CDS             complement(5578..>5796)
                     /locus_tag="VF874_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9177784.1"
                     /translation="FLAQLEQDGITPPSAKQAIKDAKAVCSMLDDGYSADEVIDAVAE
                     STGLDADGSNTFAVDAASAYCPEYVTES"
BASE COUNT          893 a         2012 c         1903 g          988 t
ORIGIN      
        1 tttctggccc agctggaaca ggacggcatc accccgccca gcgcgaagca ggccatcaag
       61 gacgccaaag ccgtctgcag catgctcgac gacggctact cggccgacga ggtcatcgac
      121 gcggtcgccg agtccacagg tctggacgcc gatggcgcca acacctttgc cgtcgacgcg
      181 gcctccgcct actgcccgga gtacgtcacc gagagctgac cagccctcac cagtgcgggg
      241 accccacccg gggcccccgc actttggcgt tcaggtgccg cccgatccgc cgactctcag
      301 ttagggcctg tggccgacgg cgaacacccg ccggaacggg aacaccgtgc cgtgcggccc
      361 cggcgggtac gcctcgcgaa gctcgacagc cagttgcgcg gcgaactccg cggcgtcatc
      421 cggcgatagc gcggccagga tcggtcgcag gccggtgccg cggatccatt ccaggaccgg
      481 gtcgtcaccg gtcagcacgt gcagatacgt cgtctcccac acatcggcgc gcagcccggc
      541 gtcgagcagc gtcgcggcgt aggactccgg cgtaccgacc gcgtcggtgt ggtgcacgac
      601 cccacgcagc ttcgcctccc accgcgactc ccccgcgagc cggcgcatca gcacatgcga
      661 cgacgaatcg aagttgcccg gcacctgcac ggctagccac ccaccgctgg gcagtgccga
      721 cgcccaccgt gtcatcagcg actggtgacg cggaacccat tgcagcgcag cgttactcac
      781 gatcacgtca acgtcacaag tgggttgcca gtcaccggcg tcggcgaccg agaacgtcac
      841 cccggccacg tcggcggcat ccccgatcat ctccggcgac gagtcgaggc cctcgacgat
      901 cgcgtcgggc caacgctcgg ccagcgttgc cgtgagatgg ccttggccac agccgacgtc
      961 gacgacgtgc cggggtgtgc tggcttcgat ccgcgcggtc agatcgaaga agggccggtc
     1021 gcgttcggcg gcgaagcggc cgtactgcgt gggatcccat cgcacggcct gaccttaacc
     1081 gcccgtccga ccggttaagg tcaggcgaag cggtcaatcg tcccgcgacc aggaggaccc
     1141 tgatgcaccg acgcctcgtc ccactggccg tgctcgcggc cgtcgtgttc gccgtcgtcc
     1201 cggcgtgctc gacgaagaaa gcgccggaag agtcgccgac cccgtccacg acgtcggcgc
     1261 caccgcagtc gcacggggtg tacgagtcgt gcctgaccca gcacggcgtc ccgtccccgc
     1321 cggccgggcc gcctccaggc ccggagccgg tgcctgcggg tccgctgccc gggccggtcg
     1381 agggcaccac cagcgccaac cccgcgccgc caccgcccgg cgtggatcag agcacctggg
     1441 acaacgcgct gaaggcctgt gcgtcgctgc aaccgaccac tccgacatcg acccgatgac
     1501 cagaaaggcc agccggcagt gacgtcaagc gctgaccccg tcgatccacc gctaccggtc
     1561 ccgaacgttc ccggcgcaga cgccggcgcg cgcgggctgc cggaccgctc ggaactcagc
     1621 ctcggtcatc ggttgatcgt cgacgcctcc gcggtcgccg acatcggtct gcgcacgttc
     1681 gtggcgtcgc tggtcggcgc ggcgatgctg ccgccgatcg cggcggaatt ggtgcgccgc
     1741 tccgataccg gccgcgagcg cgacaacatg cgcttctacg ccgagctggc cgccgagcgc
     1801 aatccggtcg cgtcgttccc ggcgccgcag cagccgccgc gcatcttttc gcggccggcc
     1861 aaccccgtcg ccgagtggct cgcccacggc cgggtgcaca acatccagtt cgacagcagc
     1921 ttcgaacccg tgaacccggc catgcgcgac cggtggcgtc gcctcgagcg caacaacgtg
     1981 gtgcgggcac agcactggcg tcacgaggac ggaccgcatc cgacgctgtg cctgatccac
     2041 ggcttcatgg gctctccgta tctcctcaac gggctgtgga tgtcgttgcc ctggttctac
     2101 cgctccggtt acgacgtgct gctgtacacc ctgccgttcc acggccggcg cgccgaaaag
     2161 tattccccct tcagcggata cggctacttc gcgaatgggt tgaccggatt cgccgaggcg
     2221 atggcgcagg cggtgcacga cttccgatca ctgatcgact atctgcaatt cgccggtgtc
     2281 gagaagatcg cgctgaccgg catctcgctg ggcgggtaca ccaccgcgct gatcgccgcc
     2341 gtcgaagacc ggctcgctgc cgtcatcccg aacgttccgg tggtgtcgat cgaggacgaa
     2401 ctcgacgact ggttcccggc gagcaagttg ttcacgttgg gccgcaagct cagccgcatc
     2461 gaccgcgagg agttcatcgc cgcgtccgcc taccattcgc cgctcaccta cccgccgctg
     2521 gtgccgcgcg accgtcggct catcatcacc ggcctcggtg accggctcgc gccgcccgag
     2581 caggccgaga tgctttggca ccactgggat cactgccacc tgcactggtt ccccggcaac
     2641 cacatccttc acgtcagcca gccggagtat ctgcgccgca tgacgcggtt cttgcgcgac
     2701 ttcatgttcg actagcgtgt cccacggccc gcgtgtctct ggtgcgggat gattccgaca
     2761 tgacgatgcc gggcttggag ctgcttgtcc gcgtcgggtt ggccacgctg ctcggcatcg
     2821 cgatcggcat ggaacgtcaa tggcgggcgc ggatggccgg cctgcagacg atggcattgg
     2881 tcagcatggg cgccgcgctg ttcgtgatcc tcggcgctta cgcattcaag ggtgactcgg
     2941 atcccacccg ggtggctgca caggtcgtca caggcatcgg tttcctcggc gcgggcgtca
     3001 tcatgaagca gggttcgtcg gtgacggggt tgaataccgc ggcgacgctg tgggcaaccg
     3061 ccgcagtggg ttcgcttgcg ggagcgtgga tgtggcggga ggcggtggtg ggagccggaa
     3121 tcatactcgc cggcaacaca tttctgtacc cgcttgccca ccggatggat cgcgcgcgat
     3181 cacgagcggg tcgcgagatc ccgcctactg attatgtttt cgaggtggag tgcgcaaccg
     3241 aggctaacgc tcgggtcaag gccttgctac tcgacgcact ctcacagacc gacctgcatt
     3301 tgacgtcctt gcgatcggcg ggcacgacat cgccggacga gatcgagctt cgcgcagagc
     3361 ttttcgcgcc cgcgcgtcac gacgagctcc tcgaaagcgc cgttcgcgcg gtcagcggcg
     3421 atcccgatgt cagatcggtc cgctggtcgg tgctgaacga ggccggcgtc cggtggagtt
     3481 gagctagcgc ttcgccgcgg tgaccaagct gccggtgcgc aacatctgcc caccgtcggt
     3541 atccggcggc ggcaccggct gacggatctg ccggaagtaa tcggccgtca cgatcccctc
     3601 gacggtccag ccgtgctgac cgaaccactt cactgcgtca cgatgcttct cgttgtagat
     3661 cagcgtgaag aacgtgcccg gttggttgcc tgccgcgcgt tcctcggcac gcttggcctc
     3721 gagatcttct tcgtcgagcg ggtgcatctc ctccaacgcc gcccggctgc ctggcgcggc
     3781 cagcgagtcg atgccggcat agagctgctc ttgcgccgtc gccggcagat acatcagaac
     3841 cccctcggcc agccacgctg agggctgagc tggctcaaac ccgctgtccc gcaatgcttt
     3901 cggccagtct tcgcgaaggt cggcggccac ctcgcggcgc tttgcggtgg ggagatcgcc
     3961 gtggtcggcc aagaccgtac gtttgaactc gagcactttg ggctgatcca gttcgaacac
     4021 gacggtgctg tccggccacg gcagccggta cgcgcgggaa tccagccccg cggcagggat
     4081 cacgatttga ctgacaccct cggcggcggc gtttcgaaag aagtcgtcga agaagcgggt
     4141 gcgggcgcct tggtaggtct ggaagacctc gccgaattct gacctcagct tgtggtcggg
     4201 cgatttgctg tcgagcacgt cggcccataa gccgcccacc gcccggcaga acacctcggc
     4261 gtacgggtcg acggccagcg gttcgtcgca ttgtgcgtga agggcccgcg ccgcggccac
     4321 gaacaacgcg gtggacccga cgctggtggt gatgtcccag gtgtcgtcgt cggtacgcac
     4381 tgtgtcgacc ctacgcgtgc tgccgcgtcg ccctttgccc gcggccggta accgattgtc
     4441 ggtttccttc gttaactaaa ctaagctaag tagccgagca acgttgacca tggccaccga
     4501 aggttgtgtc gatccgcacg cataatgagt gatacgggcg tttccgcgcg gatcgcccgc
     4561 cgaataacca cagacagcga ccgggatatg agtgacatac ggtttgcacg tttgcacagc
     4621 gaccgtgtcg cctatcgcga cgagggcgac ctagcggccg agccgctggt gctgatccat
     4681 gggatggccg gcagttcgct tgcatgggtc ccggtgatcg ggcccctgtc agcgaagtac
     4741 cgggtcatcg cgcccgatct gctcggccac ggccagtccg acaagccccg cacggactac
     4801 tcgctgggcg cgttcgcggt gtggttgcgt gatttcctcg acgagctcga cgttccctcg
     4861 gcaacgctca tcggacactc gctcggcggc ggggtggcat tgcagttcgc ctaccagcac
     4921 cgccagtact gccggcgtct cgttctggtg aacagcggcg ggctgggcac cgacatcagc
     4981 atgccgctgc gtctgctctc gacacccggc gctgaattgc tgctccccgt gctggcatcg
     5041 cggccggcga tgttcgtggg caacggggtc ctttcgctgc tcgccgcccg gggagtggag
     5101 tcgcccaaag tgcaggatcg ctggcggaag tattcggcat tggcggatcc gctcgtccgc
     5161 caggcttttc tgcggacgct gcgctccgtg gtggacctcc gcggccagac ggtctgcgcc
     5221 ctcgatcggc tgccgcgcct cgtcggcgat ctgccgacgt tgatcgtcgc cggtgcgcgg
     5281 gacgcagtca tccccgccga ccatgcggag gcggcacatg ccgttttgcc gggcagccgg
     5341 ctggagatca tcgacgacgc cgggcatcat ccccagctcg actgtcctga ggcccttgcc
     5401 cagctcatcg acgagttcgt ctcctccgat gaccgggcct tgtcacgtcg cggagccgca
     5461 ggggcggcga catcgggctc cggtcgggcg aggctgtggc atcgacccac accgcgcggc
     5521 gcctgaacgc caaagtgcgg ggaccccggc tggggtcccc gcactggtga gggctggtca
     5581 gctctcggtg acgtactccg ggcagtaggc cgaggccgca tcgaccgcga aggtgttgga
     5641 gccatcggcg tccaaacctg tggactcggc gaccgcgtcg atgacctcgt cggccgagta
     5701 gccgtcgtcg agcatgctgc agacggcttt ggcgtccttg atggcctgct tcgcgctggg
     5761 cggggtgatg ccgtcctgtt ccagctgggc cagaaa
//
LOCUS       DASWBF010000480        18700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_783330,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000480 DASWBF010000000
VERSION     DASWBF010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18700
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783330"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1830
                     /locus_tag="VF874_17505"
     CDS             214..1830
                     /locus_tag="VF874_17505"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9177785.1"
                     /translation="MGCRRPAPSLLFTAVAATVLLLPWAIDGVTDGRSDKVTADDTAL
                     ARQPLANLGAGETIREVSQATPFSLVALTGTSLDTTARVRAKRPDGSWGPWYEVETSE
                     SVGPDSSGSPRGTEPVFVGRTTAVQIAINQPASPPAASAPSPAPKDGRPDLGYKPANV
                     EQPFPQNVNAILISPPQAPADQFGLPTAVTPPGQPPAIISRAQWGADESMRCGPTRYD
                     NGIRAAIVHHTAGSNDYSPQDSAEIVRAIYAYHTRTLGWCDIAYNALVDKYGQVFEGR
                     AGGITKAVEGSHTGGFNVDTWGVAMLGDFDAVPPTPIQLRTVARLLGWRLAMDGVDPR
                     GTVTLTSAGSAFTKFMTGAPATLPTIFTHRDVGATDCPGNAAYAEMDQIRDMAARFND
                     PPGPQDLADSLQGGAIYARWQSMGAMNGWLGAPTSPESPAEGDARYVRFQHGAVYWSP
                     RTGANPVGGAIYDAWAALGYERSPLGLPTSGEIAEPEWIVQNFQHGTLNFDREKPRVI
                     QVIDGLAYELPPPSPKGPPPQVERFTSINS"
     gene            complement(1853..2668)
                     /locus_tag="VF874_17510"
     CDS             complement(1853..2668)
                     /locus_tag="VF874_17510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9177786.1"
                     /translation="MNPPALIASDVDGTLLDADELVTARTRAAVRAAIAGGATFVLAT
                     GRPPRWIGMVVDALGFAPMAVCANGAVLYDPGEDRIISARTLSADMLAELAEIATRAI
                     PGAGLAVERVGHSAHDAATPQFVSSPGYEHAWLNPDNTEVSLQDLLSAPAVKLLIRKP
                     GARSADMAAALAEHIGIEGDITYSTNDGLIEIVPLGISKATGVEELAHPLGITAEDVV
                     AFGDMPNDVPMLSWAGHGVAMGNAHPEVLAVANEVTAPNTSDGVAQVLERWWL"
     gene            complement(2691..3440)
                     /locus_tag="VF874_17515"
     CDS             complement(2691..3440)
                     /locus_tag="VF874_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9177787.1"
                     /translation="MAEPVMRMLEISVTSAVRTVGPRITLHGLENIPDTGGAVIAVNH
                     TGYVDWMPASIAAYKRKRRLRFMIKSEMLEVKPVGFLMRHSSMIPVDRSAGAGAYTVA
                     VERLREGELVGVYPEATISRSFELKEFKTGAARMAREAGVPIIPLIVWGAHRMWTKDH
                     PRKLLRNRVPITVIVGPPLRPIDSVEQTNAALREAMTELLQRAQVGYPRPAGAYWVPQ
                     RLGGSAPTLPEAKQLDEAELAERARKRAESQ"
     gene            complement(3444..4250)
                     /locus_tag="VF874_17520"
     CDS             complement(3444..4250)
                     /locus_tag="VF874_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9177788.1"
                     /translation="MEPVYATIIQAARLIWKIQGLTFTVSGVEHLPATGGAVIAINHT
                     SYFDFTFAGLPAYQQGLGRKVRFMAKKEVFEHKITGPIMRSLRHIPVDRDKGAESFER
                     ACQALKDGELVGVYPEATISRSFEIKEFKSGAARMAQAAGVPIVPHIVWGAQRIWTKG
                     YPRKMWRPKVPISVAVGEPIPATLPAPELTRLLHSRMQHLLGQVQDAYGPYPPGEFWV
                     PHRLGGGAPTLAEAARMDADEAAERAARRAASRASDDSAERQSGQTGQPE"
     gene            4337..5887
                     /locus_tag="VF874_17525"
     CDS             4337..5887
                     /locus_tag="VF874_17525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9177789.1"
                     /translation="MQVTSIGHAGFRIDTRAGSILCDPWLNPAYFGSWFPFPDNSGLD
                     WDALGDCDYLYVSHLHRDHFDPKNLRDHVNKDAVVLLPDFPVEDLRRELEKLGFSNFF
                     ETTDSVKHRVSGPKGDLDVMVIALRAPADGPIGDSGLVVDDGQTVAFNMNDARPVDLD
                     VLPADFGRVDVHMLQYSGAIWYPMVYDMPPRAKAAFGTQKRRRQMDRCRQYIAQVDAT
                     WVIPSAGPPCFLDPDLRELNDDHGDPANIFPDQLVFLAQMRAHGHDGGLLMIPGSVAN
                     FAGSQLDSLTHPMPDNEVEAIFTTGKAAYIAEYADRMAPVLATEKAGWAPADGEPLLE
                     PLRALFEPIMTQSDQISDGIGYPVELRLGPETVVLDFPKRSVREPIPDEKFRYGFAIA
                     PELVRTVLRDDEPDWVNRIFLSTRFSAWRVGGYNEYLYTFFKCLTDERIAYADGWFAE
                     THDDTATVVLDGWELQRRCPHLKADLSTFGVVEGNTLTCNLHGWQWKLDTGRCLTARG
                     HELRCAPA"
     gene            5884..6219
                     /locus_tag="VF874_17530"
     CDS             5884..6219
                     /locus_tag="VF874_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177790.1"
                     /translation="MTTPFQYYDDGLVQLDGEALTLRRYHFPSGTSKVIPVKSIRGYS
                     AKPLGLLMNRFRLWGSPDLMRWLPLDIGRPLKSTLITLDVPGTRPKPAFTPNDPDWFI
                     EVLDSLLNR"
     gene            complement(6216..7475)
                     /gene="serS"
                     /locus_tag="VF874_17535"
     CDS             complement(6216..7475)
                     /gene="serS"
                     /locus_tag="VF874_17535"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX9177791.1"
                     /translation="MIDLRQLREDPDAVRRSQRARGDDPGLVDALLDAEAARRAAISA
                     ADNLRAEQKAMSKQVGAASREERPAVLERAKQLAVGVKAAEAEQTAAEEAFTAAHMAV
                     SNVVLDEVPPGGEDDYAVLDTVGSIPAIDNPKDHVELGESLGLFDIERGAKVSGSRFY
                     FLTGRGALLQLGLLQMAVRLAVDNGFTLMIPPVLVRPEIMSGTGFLGAHADEVYRVES
                     DDLYLVGTSEVPLAGYHAGEILDLSGGPLRYAGWSSCFRREAGSYGKDTRGIIRVHQF
                     DKVEGFVYCHPDDAVQEHESLLGWQRDMLAMIEVPYRVIDVAAGDLGASAARKFDCEA
                     WIPSQQAYRELTSTSNCTTFQARRLQIRYRDANGKSQIAATLNGTFATTRWLVAILEN
                     HQQPDGSVRVPAAMVPHVGVEVLEPPG"
     gene            7636..9009
                     /locus_tag="VF874_17540"
     CDS             7636..9009
                     /locus_tag="VF874_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEX9177792.1"
                     /translation="MSEAPEQQQPSDSQPSRRWLWWRSLQATATRRALLLTALGGLLI
                     AGVTTALPAGANDAGRLVGHSNAVPEANRASQAFDNARPGDCLNWPNKEPDQATVVDC
                     KDEHRFEVATSVDMRMFPGSEYGPNAAAPSAARVQQISQEQCQAAIKRYLGTKYDPNS
                     KFTIGMLWSGTKAWSSGDRQMLCGLQLPGGGNEEQQAVKGRVADLDQSKVWQVGTCLG
                     IDTAANQPTDIPIDCAGPHAMEVTGSVNVAEKFPGGALPPEADQDAYIKDTCTRMTDA
                     YLAPVELRTTTLTLVYSTVSLPSWSAGSHQVSCNIGATLGNGGWATLVGSAKGALLIN
                     GQPPVPPPDIPAERLNIPPMPMPMPMPMPTTTTIDTSGSQSQLPQQTLSPTNAVPTIG
                     NQHLPNAPGPQTVDSPAPAAPVESPAPAAPPQPEPVVPPPFGAPPPAPEPILPPAAPA
                     ELPPPPA"
     gene            9014..9364
                     /locus_tag="VF874_17545"
     CDS             9014..9364
                     /locus_tag="VF874_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX9177793.1"
                     /translation="MAVRMSPERFDELVSDALDLIPPDLAAAIDNVVVLVEARNAAEP
                     DLLGLYEGIALTERDTTYAGALPDTVTIYRDALLDICETEDDVVDEVAVTVIHEIAHH
                     FGIDDERLHELGWA"
     gene            9430..9621
                     /locus_tag="VF874_17550"
     CDS             9430..9621
                     /locus_tag="VF874_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177794.1"
                     /translation="MSLQCRYCRDGWEHCHGTVIVHVQHRAECTEDGCVNPESIAHSL
                     RIDCEAVGCSCGQPIALAV"
     gene            complement(9629..10315)
                     /locus_tag="VF874_17555"
     CDS             complement(9629..10315)
                     /locus_tag="VF874_17555"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9177795.1"
                     /translation="MSGRLVLLRHGQSYSNVERCLDTRPPGASLTPAGIAQARDFARG
                     FERPAGMLVHSVALRARQTASVIGEVWKIRPHEVEGLHEAQVGELEMRNDDEAVDAFH
                     EVYERWHSGDLDAALPGGETGAQVLGRYLPVLSDLRMRYLDDHEWTGDIVVVSHGAAI
                     RLAVATLAGVDGSFVVEHHIGNAESVVLAPITDGRWSCVHWGQLSPPFLPDPRGMPRP
                     DELESADPMG"
     gene            complement(10312..11235)
                     /gene="pheA"
                     /locus_tag="VF874_17560"
     CDS             complement(10312..11235)
                     /gene="pheA"
                     /locus_tag="VF874_17560"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX9177796.1"
                     /translation="MTRIAYLGPEGTFTEAALLQMVDRGMIPRDTVIEPLATDGTAAA
                     LQQVRDRAAEYACVPIENSIDGSIAPTLDSLATGTPLRLFAELTLDVAFSIAVRPGIA
                     EADVRTIAAFPVAAAQVRRWLAQNLPAATVVPANSNASAAVDVRDGKADAAVTTSLAA
                     RRYGLAELAAGVVDEPNAATRFVLAGLPARPPDRTGADRTSVVLRLENRPGALVEALT
                     EFGIRDIDLTRIESRPTRTELGTYVFFLDCVGHIDDSAVAEALKALHRRCADLRFLGS
                     WPTGSVTGAVPPEMDEAGRWLQRLRDGGGKT"
     gene            11337..12027
                     /locus_tag="VF874_17565"
                     /pseudo
     CDS             11337..12027
                     /locus_tag="VF874_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
     gene            12144..12902
                     /locus_tag="VF874_17570"
     CDS             12144..12902
                     /locus_tag="VF874_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX9177797.1"
                     /translation="MTEPAAELPRRTLRLEVVIVLGLTFGLSAVTAGLQLVDSVLRTL
                     SAQKVPLIERRSYFDLVDLGLNLASITQLLAWGALGLYLLWRSGIGPRDIGLSRPRWR
                     ADVLGGLGLAALIGVPGLGLYVLSRVIGVSASVVPTTLTDTWWRIPVLILAAFANGWA
                     EEVIVVGYLMTRLRQLGVGSSRTLVYSSLLRGAYHLYQGFGAGLGNFAMGLVFGVVWR
                     RHGRLWPLIVAHGVIDTVAFVGYAVLAGHLGWLP"
     gene            12983..14146
                     /locus_tag="VF874_17575"
     CDS             12983..14146
                     /locus_tag="VF874_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX9177798.1"
                     /translation="MHHRGGPPIYRRPVPPPGRSEPSQVIRRDPTYRPPPPPTQQRPT
                     GPPRPAPPRPPVAPPARPGPPKPRRKRHWGRWVAVAVLAVLVAFVGFGVWVDRSLHRA
                     DVVTAYPGRPAPGRGTTWLLVGSDSRSDLSPEQQDALATGGDVGDRTDTIMLIHIPAL
                     TSDVPATVVSIPRDSQVSVPGNGENKINAAFTLGGPQLLVQTIEQATGIRVDHYAQIG
                     FAGFADVVDAVGGVRMCPAEPINDPLAGIDLAAGCQKLNGRNALGYVRTRATPRADLD
                     RMVNQREFLSALLHRAASPAVWLNPFRWRSLKDSVTNALTVDPGVHVWDLARLAWALR
                     GSPVTTTVPIEGSAVTDLGDVVEWDSDAASRLFSALSTDAPVPQDVLESQPTG"
     gene            complement(14152..14970)
                     /locus_tag="VF874_17580"
     CDS             complement(14152..14970)
                     /locus_tag="VF874_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX9177799.1"
                     /translation="MTSAGGPRAHPFVVAHRGASGDRPEHTLAAYEIALQQGAEGVEC
                     DVRLTRDGHLVCVHDRRVDRTSTGTGLVSDMTLEQLRALDYGAWHNSWRPDGRHGDTG
                     LLTLDALVGLVLDWRRPLKIFIETKHPVRYGSLVESKVLALLHRFGIASPASADRSRA
                     VVMSFSAAAVWRIRRAAPMLPTVLLGDTSRYLGGSAATTVGATAVGPSIRTLREHPEL
                     VDRAAAQGRATYCWTIDHLEDVDYCRDLGVGWIATNHPGRTKGWLHRGLSELHP"
     gene            complement(14976..15995)
                     /locus_tag="VF874_17585"
     CDS             complement(14976..15995)
                     /locus_tag="VF874_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX9177800.1"
                     /translation="MIQVCSRCGTRWNVRDRQRQWCPRCRGALLPPDTSHQWSSAPTT
                     PGLQPSRPRSGPLLPAGYRWIAVRPGSGPPPRRRRRALGPTPRYAFIPRWGLVDPATQ
                     LPATPSAPPRRGPSSRAVRAMLITTIAVFGVAAFLHIVRYALLIVNRGVLLNSVVAGL
                     ATWVAVMASVGAMFAIVGTYLVLTEWLLARRATAFAHYYQGDPRPAWELRVGCLAPLM
                     NLALPLTYVVELAAAEEKYRHLRRLIWAWWVLLLLSFAASMFAAATSFPDNTQGIADN
                     TVSFVVAYLLGMAAVVATARLVFAFERTPVERPAHRWVMVPDAKQSRPDSPTAVEREG
                     QEPAA"
     gene            16488..16646
                     /locus_tag="VF874_17590"
     CDS             16488..16646
                     /locus_tag="VF874_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177801.1"
                     /translation="MAAHPIGSNPAMPPSEWETSSPVYARSQMTSALRAALIALVLLA
                     ILALIVLF"
     gene            16794..17417
                     /locus_tag="VF874_17595"
     CDS             16794..17417
                     /locus_tag="VF874_17595"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX9177802.1"
                     /translation="MAEYTLPELPYDYGALEPHISGQINELHHSKHHATYVKGANTAV
                     EQLAEAREKGDHASIFLHEKNLAFNLGGHVNHSIWWKNLSPEGGDKPTGELPSALDDA
                     FGSFDKFQAQFTAAANGLQGSGWAVLGYDSLGDKLLTFQLYDQQANVPLGIIPLLQVD
                     MWEHAYYLQYKNVKADYVKAFWNVVNWADVQDRFAAATSKTKGLIFG"
     gene            17601..18134
                     /locus_tag="VF874_17600"
     CDS             17601..18134
                     /locus_tag="VF874_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9177803.1"
                     /translation="MSTGSGRAIEVAPFHSRGSLKGFVVSGRWPDSTKEWAQLLIVAV
                     RVASLPGLLSTTTIFGVREELPEEPEPGTVGLVLAEGPVVGESALPPGHFADHLPPAL
                     MMLHPPSETVPSLPECTGAASGCVLLPGLPHLGLEHRAAWVEAEADGTVTSMVSRVGV
                     DPISHPDTAILAMLLAA"
     gene            18366..>18700
                     /locus_tag="VF874_17605"
     CDS             18366..>18700
                     /locus_tag="VF874_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX9177804.1"
                     /translation="MLTATLVSLGVVFVAEIGDKSQLIAMTYALRHRWWVVLSGVALA
                     AFLVHGLSVTIGHFLGLTLPERPIAFAAATAFFGFALWTWRERSDDGSAPPAPEHRFV
                     LLAVVSSYLL"
BASE COUNT         2962 a         6495 c         6209 g         3034 t
ORIGIN      
        1 gtcgatcatg caggcgatca agtcagcggc acccgctgct gaggcggcac ctgccgcggc
       61 ggtgccggcg aactaagact tgtcggccaa acggcatcgc agaggtacgg cgagctctgc
      121 gatgccgttg ccgacgtcat gagcggtggc gcaacgcgca tccgcggtgt gtcgaaacac
      181 tgataacaac agtcccatgc gtaacatcgg tgcgtggggt gccgacgtcc tgccccgtcg
      241 ctgctgttca ccgcggtcgc ggcgactgtg cttttgttgc cgtgggcgat tgacggcgtc
      301 accgacggcc ggtccgacaa ggtcactgcc gacgacacag cgctcgcccg gcagccgctg
      361 gcgaacctcg gtgccggaga gacgatccgc gaggtcagcc aggccacgcc gttctccctg
      421 gttgcgctga cggggacgag tcttgacacg acggcgcggg tgcgcgcgaa gcgccccgac
      481 ggcagctggg gaccctggta cgaggttgag acgagcgagt ccgtcggccc cgacagcagc
      541 ggttctccgc gcggcacgga gcccgtgttc gtcgggcgca ccaccgctgt gcagatcgcg
      601 atcaaccaac cggcttcgcc gccggccgct tcggcgccga gtcccgcgcc gaaggacggc
      661 cgccccgacc tcggctataa gcccgccaac gtcgagcagc cgtttcccca gaacgtcaac
      721 gcgatcctga tcagcccgcc acaagcgccc gccgatcagt tcgggttgcc caccgcggtc
      781 acaccgcccg gccagccacc cgcgatcatc agtcgcgcgc agtggggcgc cgacgagtcg
      841 atgcggtgtg gtccgaccag gtacgacaac gggattcggg ccgccatcgt ccaccacacc
      901 gccggcagca acgactactc gccgcaggac tccgccgaga tcgtgcgggc gatctacgcg
      961 tatcacaccc gcacgctcgg ctggtgcgac atcgcctaca acgcgctcgt cgacaagtac
     1021 gggcaggtgt tcgagggccg ggccggcggc atcacgaagg cggtcgaagg ctcgcacact
     1081 ggcggcttca atgtggacac ctggggcgtg gcgatgctcg gggacttcga cgcggtaccg
     1141 ccgacgccga ttcagctgcg cacggtcgcg cggctgctcg gctggcggct ggcgatggac
     1201 ggcgtcgacc ctcgcggcac tgtgacgttg acctcggccg gtagcgcgtt cacgaagttc
     1261 atgaccggcg cgccggcgac gctgccgacg atcttcaccc accgcgacgt cggcgccaca
     1321 gactgtccag gcaacgccgc atacgcagag atggaccaga ttcgcgacat ggccgcccgg
     1381 ttcaacgacc cgcccgggcc gcaggatctt gccgactccc tgcaaggcgg ggcgatctat
     1441 gcccgctggc agtccatggg cgccatgaac ggctggctgg gtgcgccgac gtcgccggag
     1501 tcaccggccg aaggagacgc ccgctacgtg cgcttccagc acggcgcggt gtactggtcg
     1561 ccgcggaccg gggccaaccc cgtcggcggc gcgatctatg acgcctgggc ggcgctcggc
     1621 tacgagcgca gcccgctggg cctgccgacc agcggcgaga tcgccgagcc ggagtggata
     1681 gtgcagaatt tccagcacgg gacgctgaac ttcgaccggg aaaagccgcg ggtgatccag
     1741 gtgatcgacg ggctcgccta cgagctgccg ccgccgtcgc cgaaaggccc gccgccacag
     1801 gtcgagcggt tcacctcgat caacagctga attctcctgg cgcacaatag gtttacagcc
     1861 accagcgttc cagcacctgc gccaccccgt cgctggtgtt gggcgccgtc acctcattgg
     1921 ccaccgcgag cacctccggg tgcgcgttgc ccatcgcgac gccgtggccc gcccacgaga
     1981 gcatcgggac gtcgttgggc atgtcgccga acgcgaccac gtcctcggcg gtaatgccca
     2041 acgggtgcgc gagctcctcg acgccggtgg ccttgctgat gcccagcggc acgatctcga
     2101 tcagaccgtc gttggtcgag taggtgatgt ctccttcgat gccgatgtgc tccgccagtg
     2161 cggcggccat gtccgcgctg cgggcaccgg gtttgcggat aagcagcttc accgcgggcg
     2221 cgctgagcag gtcttgcagc gacacctcgg tgttgtccgg gttcagccag gcgtgctcgt
     2281 aaccgggtga gctgacgaac tgcggggtgg ccgcgtcgtg ggcgctgtgg cccacccgct
     2341 cgaccgcgag accggcgcca ggaattgcgc gggtggcgat ctcggccagc tccgcgagca
     2401 tgtcggcgga cagagtccgc gccgagatga tccggtcctc gcccgggtcg tagagcaccg
     2461 cgccgttcgc gcacaccgcc atcggagcga accccaacgc gtcgaccacc atgccgatcc
     2521 agcgcggcgg gcggcccgtg gccagcacga acgtcgcgcc gccggcgatg gcggctcgga
     2581 cggcggcacg ggtgcgcgcg gtgaccaact cgtcggcgtc cagcagagtg ccgtccacat
     2641 cgctggcgat cagcgcaggc gggttcaccg cgaacttctc gcggatgagt tcactggctt
     2701 tccgcccgct tccgcgcccg ctcggccagc tccgcctcat cgagttgctt ggcctcgggc
     2761 agcgtcggcg cgctgccgcc gagccgctgc ggcacccagt acgcgccggc cggtcgcgga
     2821 tagccgacct gggcgcgctg caacaactcc gtcatcgcct cgcgcagcgc ggcattcgtc
     2881 tgttcgacgc tgtcgatggg acgcagtggc gggccgacga tgacggtgat gggcacacga
     2941 ttgcgaagca gcttgcgcgg atggtccttc gtccacatcc gatgtgcacc ccagacgatc
     3001 aacgggatga tcgggacgcc ggcctcgcgg gccatccgcg cggcgccggt cttgaactcc
     3061 ttcagctcga agctgcggct gatcgtcgcc tccggataca cacccacgag ctcgccctcg
     3121 cgcagccgct cgaccgccac ggtgtacgcg cccgcgccgg cgctgcggtc gacgggaatc
     3181 atgctgctgt ggcgcatcag aaagccgacc ggcttcactt ccagcatttc gctcttgatc
     3241 atgaagcgca gccggcgttt ccgcttgtag gcggcgatcg acgccggcat ccagtcgacg
     3301 tagccggtgt ggttgaccgc gatcaccgcg ccgccggtgt cggggatgtt ctccagaccg
     3361 tgaagagtga ttctgggacc caccgtcctg actgcggacg tgacagagat ttccagcatg
     3421 cgcatcactg gctccgccat cggctactcc ggttgtccgg tctgcccgga ctgtcgctcg
     3481 gcgctgtcgt cggatgcgcg gctcgccgcg cggcgggccg cccgttccgc cgcttcgtca
     3541 gcatccatgc gggccgcctc ggccagcgtc ggggcgccgc cgccgagccg gtgcggaacc
     3601 cagaactcgc ccggcgggta cgggccgtag gcgtcttgga cctgtcccaa caggtgctgc
     3661 atccgcgagt gcagcagcct ggtgagttcc ggcgccggca gcgtcgccgg gatcggttcg
     3721 ccgaccgcca cactgatcgg gaccttcggc cgccacatct tgcgcgggta gcccttcgtc
     3781 cagatccgct gagcacccca gacgatgtgc gggacgatcg gcaccccggc ggcctgagcc
     3841 atccgtgccg ctccggactt gaactccttg atctcgaagc tgcggctgat cgtggcctcg
     3901 gggtagacgc cgacgagctc gccgtccttg agcgcctggc aggctctctc gaacgactcc
     3961 gcgcctttgt cccggtcgac ggggatgtgg cgcagactgc gcatgatcgg cccggtgatc
     4021 ttgtgctcga acacttcctt tttggccatg aaccgcacct tgcggccgag accctgctgg
     4081 taggccggca acccggcaaa cgtgaagtcg aagtagctgg tgtggttgat cgcgatgacc
     4141 gcgccaccgg tggccggcag atgctccacg ccgctgacgg tgaacgtcag cccctggatc
     4201 ttccagatca ggcgggcggc ctggatgata gtggcgtaca ccggttccac gtccgcaaga
     4261 ctaatccgcc taaccgaccc cgatgccacc tgccagtaag ctcgaaccgg cacggctagg
     4321 gcatcaaggg ggttatgtgc aggtcaccag cattgggcac gctggctttc gcatcgacac
     4381 ccgcgccgga agcatcttgt gcgacccatg gctgaacccc gcctacttcg ggtcctggtt
     4441 tccgtttccc gacaacagcg gactcgactg ggacgcactc ggtgactgcg actacctgta
     4501 cgtgtcgcat ctgcaccgcg accacttcga cccgaagaac ctgcgcgacc acgtcaacaa
     4561 ggacgccgtc gtgctgctgc ccgactttcc ggtcgaagac ctccgacgcg aactggagaa
     4621 gctgggcttc tccaacttct tcgagaccac cgattcggtc aaacaccgtg tcagcgggcc
     4681 caagggcgac ctggacgtga tggtcatcgc gctgcgcgcc cccgccgacg ggccgatcgg
     4741 cgactccggt ttggtggtcg acgacgggca gacggttgcg ttcaacatga acgacgcgcg
     4801 gccggtcgat ctcgacgtgc tgcccgccga cttcggccgg gtcgacgtgc acatgctgca
     4861 gtactccggc gcgatctggt atccgatggt ctacgacatg cccccgcggg cgaaggcggc
     4921 gttcgggacg cagaagcggc gacgccagat ggaccgctgc cggcagtaca tcgcacaggt
     4981 cgacgcgacg tgggttatcc cgtcggccgg accgccgtgc ttcttggacc ccgacctgcg
     5041 cgagctcaac gacgaccacg gcgatccggc gaacatcttc ccggaccaac tggtgttcct
     5101 ggcgcagatg cgtgcacacg gccacgacgg tgggctgctg atgattcccg gctctgttgc
     5161 gaatttcgct ggctcacaat tggattcgct gacccacccg atgcccgaca acgaggtcga
     5221 ggccatcttc accacaggca aggccgcata catcgcagag tacgccgacc ggatggcccc
     5281 ggtgctggcc accgagaagg ccggctgggc gccagccgac ggcgagccgc tgctggagcc
     5341 gctgcgggcg ctgttcgagc caatcatgac tcaaagcgat caaatcagtg acggcatcgg
     5401 ctacccggtg gagttgcggc tcgggcccga aaccgtggtg ctcgactttc cgaaacggtc
     5461 agtgcgtgaa ccgattccgg acgagaagtt ccgttacggg tttgcgatcg cgcccgagct
     5521 ggtccgtacc gtgctgcggg atgacgagcc cgactgggtg aacaggatct tcctgtcgac
     5581 gcggttctcc gcgtggcggg tgggcggcta caacgaatat ctgtacacgt tcttcaagtg
     5641 tttgaccgac gagcggatcg cctacgcgga cggctggttc gccgagacgc acgacgacac
     5701 ggccacggtg gtgctggacg gttgggagtt gcagcggcgc tgcccacacc tgaaggccga
     5761 cctgtcgaca ttcggtgtgg tggaaggcaa tacactgact tgcaacctgc acggctggca
     5821 gtggaagctc gacacgggcc gctgcctcac cgcccgtggc cacgagctgc gctgcgcacc
     5881 ggcatgacca cgccgtttca gtactacgac gacgggctgg tgcagctcga cggcgaggca
     5941 ctgacactgc ggcgctacca cttcccgtcg ggcacgtcga aggtgatccc ggtgaagtcg
     6001 atccgtggct acagcgccaa accgcttggg ctgctgatga atcggttccg gctgtggggc
     6061 agcccggatc tgatgcgttg gctgccgctg gacatcggtc gcccgctgaa gtcgacgctg
     6121 atcactctgg acgtgcccgg gacccgtccc aagccggcgt tcaccccaaa cgatcccgac
     6181 tggttcattg aggtgctgga ctcgctgctg aaccgctagc ccggcggctc gagcacctcg
     6241 acgccgacgt gcggcaccat cgcagcaggc acccggacgc tgccgtctgg ctgctggtgg
     6301 ttctccagta tcgccaccag ccatctggtt gtcgcgaatg ttccgttcag cgtcgcggcg
     6361 atctgcgact tgccgttcgc gtcgcggtag cggatctgaa gccggcgcgc ctgaaacgtc
     6421 gtgcaattcg acgtcgaggt cagctcccga tacgcctgtt gcgacgggat ccacgcctcg
     6481 cagtcgaact tacgtgccgc cgacgctccg agatcgcctg cggccacatc gattacgcgg
     6541 tatggcacct cgatcatcgc gagcatgtcc cgctgccagc caagcaggct ttcatgctct
     6601 tgcaccgcgt cgtccgggtg gcagtagaca aagccctcga ccttgtcgaa ctgatgcacc
     6661 cggatgatgc cgcgggtgtc cttgccgtag ctgccggcct cccgccgaaa gcacgacgac
     6721 cacccggcat atcgcagcgg cccgccggac aggtcgagga tctcccccgc atggtatccg
     6781 gccagcggca cctccgaggt gccgaccaga tagaggtcgt cggattccac ccggtacacc
     6841 tcgtcggcgt gcgcacccaa aaacccagtg cccgacatga tttcgggcct caccagcacc
     6901 ggcgggatca tcaacgtgaa cccgttgtcg acggccagcc gcaccgccat ctgcaacagc
     6961 cccagctgca gcagcgcccc ccgcccggtg aggaagtaga accgcgagcc cgacaccttg
     7021 gcgccgcgct cgatgtcgaa caggccaagc gactcgccca attccacatg gtctttcggg
     7081 ttgtcgatcg ccggaatcga cccgacggtg tcgagcaccg cgtagtcgtc ctcgccaccg
     7141 gggggcacct cgtcgagcac gacgttggag accgccatgt gcgcggcggt gaacgcctcc
     7201 tccgcggcgg tctgctcggc ctcggcggcc ttcacgccga cagcgagttg cttcgcgcgc
     7261 tcgagcaccg cgggccgctc ctcccgggac gccgccccca cctgcttgct catcgccttc
     7321 tgctcggcgc gcagattgtc ggcagctgag attgccgcgc gccgtgcggc ctcggcgtcg
     7381 agtaacgcgt cgacgagccc cgggtcgtcg ccgcgggcgc gctgcgagcg cctcaccgcg
     7441 tcggggtcct cccgtagctg cctcaggtcg atcacgggcg caacactact tttgcggcgc
     7501 gccgcccagc atgccgaggt agtcggtgcg acggaacggc ccagcggtgt cgtcgtccca
     7561 gcgaggcaca accccataac gttacaactg cctcacttgt cacgcagtgc gacgcgcctg
     7621 tcacaatggg gggcaatgtc cgaggcacct gagcagcagc aaccgtccga ctcccaaccg
     7681 tcgcggcgct ggctgtggtg gcgcagcctg caggctacgg cgacacgtcg ggcgctgctg
     7741 ctgacggcgc tgggcgggtt gctcatcgcc ggcgtcacca ctgcgctgcc agccggcgcc
     7801 aacgatgccg gccgcctcgt cggccactcc aacgccgtcc cggaggccaa ccgcgccagc
     7861 caggcgttcg ataacgcccg ccccggcgat tgcctgaact ggccgaacaa ggagcccgac
     7921 caggccaccg tcgtcgactg caaggacgag caccgcttcg aggtcgccac gtcggtggac
     7981 atgcgcatgt tccccggcag cgaatacggg ccgaacgccg cggcgccgtc ggccgcccgg
     8041 gtccaacaga tcagccagga gcagtgccag gccgcgatca aacgctatct cggcacgaag
     8101 tacgacccga acagcaagtt caccatcggc atgctgtggt ccggcacgaa ggcgtggtcg
     8161 tccggcgacc gccagatgct gtgcgggctg cagctgcccg gcgggggcaa cgaggagcag
     8221 caggcggtca agggccgggt cgccgacctc gaccagtcga aggtgtggca ggtcggtacg
     8281 tgcctgggca tcgacaccgc cgccaaccag ccgaccgaca tcccgatcga ctgcgcgggc
     8341 ccgcacgcga tggaggtgac cggctcggtc aatgtggccg agaagttccc cggtggcgcc
     8401 cttcccccgg aggctgacca ggacgcctac atcaaggaca cctgcacccg gatgaccgat
     8461 gcgtacctgg cgccggtgga gctgcggacc acgacgctga cgctggtgta cagcacggtc
     8521 tcgctgccca gctggtcggc gggcagccac caggtgtcgt gcaatatcgg cgccacgctc
     8581 ggcaacggcg gctgggcaac gctggtaggc agcgccaagg gggcactgct gatcaacgga
     8641 cagcccccgg ttccgccgcc cgacatcccc gctgagcgac tcaacatacc gccgatgccg
     8701 atgccgatgc cgatgccgat gccgacgacg acgacgatcg acacgtccgg ctcgcagtcg
     8761 cagctgccgc agcagacgtt gtcgccgacc aatgctgtgc cgacgatcgg caaccagcac
     8821 ctgccgaacg caccgggccc gcagaccgtc gactctccgg cccccgccgc ccccgtcgag
     8881 tcgccggctc cggcagcacc cccgcaaccg gaaccagtcg tgccgccacc gttcggtgcg
     8941 cctccgcccg cgccggagcc gattctgccg ccggctgcgc ccgcagaact gccaccgccg
     9001 ccggcgtagc ggcatggccg tgcggatgag cccggaacgg ttcgacgaac tcgtatccga
     9061 tgcgctcgac ctcatcccgc ccgacctcgc cgccgcgatc gacaacgtcg tcgtgctcgt
     9121 ggaggcccgc aacgccgccg aaccggacct gctcggcctg tacgagggca tcgcgctcac
     9181 cgaacgcgac acgacctacg ccggtgcgct gcccgacacg gtcacgatct accgcgacgc
     9241 gctactggac atctgtgaga cggaagacga cgtggtcgac gaggtcgcgg tcaccgtgat
     9301 ccacgagatc gcccatcact tcgggattga cgacgaacgg ctgcacgaat tgggttgggc
     9361 gtgacctgaa aaccacagcc tccggcgcgg cagccgcggg ttgtcggtcg gcggtgatat
     9421 gaacaggcca tgagtctgca gtgccgctac tgccgtgacg ggtgggagca ctgccacggc
     9481 accgtcatcg tccacgtgca acaccgcgcg gagtgcaccg aggacggctg cgtcaacccg
     9541 gaaagcatcg cgcactcgtt gcgcatcgat tgcgaggcgg tgggctgcag ctgcgggcag
     9601 cccatcgcgt tggcggtgta gaagcccgtc agcccatcgg gtcggcggac tccagttcat
     9661 ccggtcgcgg catccccctc ggatcgggca ggaacggcgg gctgagctgg ccccagtgca
     9721 cgcagctcca gcgtccgtcg gtgatcggcg ccaacaccac cgactcggcg ttgccgatgt
     9781 ggtgttcgac cacgaacgac ccgtccactc cggccagcgt ggccacggcc agccggatcg
     9841 cggcgccgtg gctgaccacc acgatgtcac cggtccattc gtggtcgtcg aggtagcgca
     9901 ttcgcaggtc ggacagcacg ggcaggtacc ggcccagtac ctgggcgccg gtttcgccgc
     9961 cgggaagcgc ggcgtcgagg tcgccggaat gccaccgctc gtagacctcg tggaacgcgt
    10021 cgacggcctc atcgtcattg cgcatctcca gctcgcccac ctgcgcctcg tggaggccct
    10081 cgacttcgtg aggacggatc ttccacacct cgccgatgac cgacgcggtc tgcctggccc
    10141 gcaacgcgac cgagtgcacc agcatgccgg cgggccgttc gaacccgcgc gcgaagtctc
    10201 tggcctgcgc gatccccgcg ggggtcagcg acgcccccgg cggacgcgtg tcgaggcagc
    10261 gctcgacgtt gctgtatgac tgaccgtgac gcagcagtac caacctcccg ctcatgtttt
    10321 cccgcccccg tcgcgcagcc gctgcagcca ccggccggcc tcgtccattt ccggcggcac
    10381 agcgccggtc accgagccgg tcggccatga acccaggaat cgcagatctg cacaacgacg
    10441 gtgcagcgcc ttgagtgcct cggcaaccgc gctgtcgtcg atgtgtccga cgcagtccag
    10501 gaagaacacg taggtgccca gttccgttcg ggtgggccgg gattcgatcc gggtcaggtc
    10561 gatgtcgcgg atgccgaact cggtcagcgc ctcgaccagc gccccgggcc tgttctccag
    10621 ccgcagcacc accgaggtgc ggtcggcgcc ggtgcggtcg ggcgggcggg caggcagacc
    10681 cgccagcaca aatcgggtgg cggcgttcgg ctcgtcgacg acgccggccg ccagctcggc
    10741 gaggccgtaa cgccgcgcgg ccagggacgt ggtcaccgcg gcgtccgcct tgccgtcccg
    10801 gacgtcgacc gcggcggacg cgttcgagtt cgccggcacg accgtcgcgg cgggcagatt
    10861 ctgtgcgagc caacgtcgca cctgcgccgc agcgaccggg aatgcggcga tcgtgcgcac
    10921 gtcggcctcc gcgatccccg gccgtaccgc gatgctgaat gcgacgtcga gggtcagctc
    10981 cgcgaacagc cgcagcgggg ttcccgtggc caggctgtcc agcgtcggcg cgatcgatcc
    11041 gtcgatcgaa ttctcgatcg gcacgcatgc atactcggcg gcgcggtcgc ggacctgctg
    11101 cagcgccgcc gcggtgccgt cggtggccaa cggctcgatc accgtgtctc gcgggatcat
    11161 cccgcggtcc accatctgca gtaacgcggc ctcggtgaac gtcccctccg gtccgaggta
    11221 cgcgatgcgg gtcaccgtca aactctatcg gggcgacgca aagcaacccc ttgcgcattt
    11281 gccgtcatgt tattacttta ggcttacctc actttacaaa tatgggaagg aacgtcatgc
    11341 ctcagccggc cccgtccacc gccgaacacg tccgcagcat ctgtgtgggc gcgtcgggag
    11401 cgatgctggc ggtcgacggc tttgcgcccg tggacacagc cgtccatcac ctgctcggcg
    11461 acggcgttgg agttgaccga ccatgcaccg cttgcgctgc gccggcccgt gagatcgctc
    11521 gtgtggatcc gcggccggct ccgtcgcgtc cctgagccgc tggtggcggc cctgctcgac
    11581 gtgatcgccg ccgacaaccc caatcctgca ctgctgcaag tgaattcgct cagtagccgc
    11641 ctgtcgctga tgtggttgca cacggagtcg gtggtaatcg cagacgcgac cggggcggag
    11701 tcggttgcgg tggaggacct gctggcagcc cggcctgatc cgttctgcgc gatggagtcg
    11761 tgctggctgc agcacatcga caccgcccac cgcgaggtgg tcgacctgct ggcatcccgg
    11821 ttgccggcgg ggctgcgccg cggccaagtc cggccgctcg gcctggaccg ctacggggta
    11881 cggctgcggg tcgagcacga cgacagcgat cacgacgtac ggctgccgtt cgccgcgccc
    11941 gttcacgacc tgaccgggtt gggccgcgcg atccgggtgc tgatgggctg cccgttcctc
    12001 aacggtcttc gcgcccgaag agcctagaac cggctacgag ttgcgccgat ttctgcacca
    12061 gggctgcgat ttcgaccgat ccacagccct gacgcagatc tcgacggctg gtcggcgtgg
    12121 gttcgttcgc taccgtatct ccggtgaccg agcctgccgc cgaattgccg cggcggacat
    12181 tgcggctcga ggtcgtcatc gtcttgggtc tgacgttcgg gctttccgcc gtcaccgcgg
    12241 ggttgcagct cgttgactcg gtgctgcgca ccctcagcgc gcagaaggtc ccgctgatcg
    12301 agcggcgctc gtacttcgac ctagtggatc tcgggctgaa cctggcgtcg atcacgcagc
    12361 tgctggcatg gggcgcgctg ggcctatacc tgttgtggcg cagcgggatt gggcctcgtg
    12421 acatcggact gagccggccc cgctggcgcg ccgacgtgct cggcggcctg ggtctggccg
    12481 cgttgatcgg ggttcccggg ctgggcctgt atgtgctctc gcgcgtcatc ggcgtcagcg
    12541 catcggtggt gccgaccacg ctcaccgata cctggtggcg aattccggtg ctgattctcg
    12601 ccgcattcgc caatgggtgg gctgaggaag tgatcgtggt cggctacctg atgacgcggc
    12661 tgcgtcagct cggggtgggc agcagccgca cgctggttta ctccagcctg ctgcgcggcg
    12721 cgtatcacct gtatcagggt ttcggtgcag ggctgggcaa cttcgcgatg ggcttggtgt
    12781 tcggcgtcgt gtggcggcgc cacggccggc tgtggccgct gatcgtcgcg cacggcgtga
    12841 tcgataccgt cgcgttcgtc ggctacgcgg tgcttgccgg tcacctcggc tggctgccgt
    12901 aaggccgtag attgttccgg tgactcgcga ctggccggag cacggcggcg ggccgcccat
    12961 gcagcacggc agcgtgccgc ccatgcatca ccgcggcggg ccgcccatct accggcggcc
    13021 tgttccgcca ccgggccgct ccgagccgtc acaagtcatt cgacgcgacc ccacgtatcg
    13081 cccacctccg ccgccgactc agcagcggcc gactggcccg ccgcggcccg ccccgccacg
    13141 tccgcccgtt gcgccgcctg cacggcctgg accgccgaag ccgcgtcgca agcgacattg
    13201 gggccgctgg gtcgcggtag ccgtgctggc cgtgctggtg gcgttcgtcg gtttcggggt
    13261 ctgggtcgac cggtcgctgc accgcgctga cgtcgtcacc gcctacccgg gccggcccgc
    13321 acccggccgc ggcaccacgt ggctgctggt gggatcggac agccgaagcg atctatcgcc
    13381 cgagcagcag gacgcgctgg cgacaggcgg tgacgtcggc gatcgcaccg acacgataat
    13441 gctgatccac atcccggcgc tcacctccga cgtaccggcc acggtggtgt cgatcccccg
    13501 tgactcccag gtgagcgttc ccgggaacgg cgagaacaag atcaacgccg ccttcaccct
    13561 cggtggcccg cagctgctgg ttcagaccat cgagcaggcc accggcatcc gggtggacca
    13621 ctatgcgcag atcgggttcg ccgggttcgc cgacgtcgtc gacgccgtcg gcggcgtgcg
    13681 gatgtgcccg gccgagccga tcaacgatcc gctggcgggc atcgatctgg ccgccgggtg
    13741 ccaaaagctc aacgggcgca acgcacttgg gtacgtgcgc actcgcgcca ccccccgggc
    13801 cgacctcgac cggatggtca accagcgcga gttcctctcc gcgctgcttc accgcgcggc
    13861 gagccctgcg gtgtggctga acccgttccg gtggcgctcg ctgaaggact cggtgaccaa
    13921 cgcgttgacc gtcgaccccg gcgtgcacgt gtgggacctg gcgcgactgg cgtgggcgct
    13981 gcgtgggtcg ccggtcacga cgacggtgcc gatcgagggc tcggccgtca ccgacctcgg
    14041 cgacgtcgtg gagtgggaca gcgacgcggc gtcgcggctg ttctcggcgt tgtccacgga
    14101 cgcgccggtg ccgcaggacg tgctggagtc gcagccgacg ggctgacgag ctcaggggtg
    14161 cagctcggag agccctcggt gcaaccagcc cttcgttcgg ccggggtgat tggtcgcaat
    14221 ccatcccact cccaggtcgc ggcagtaatc gacgtcctcg aggtgatcga tcgtccagca
    14281 gtatgtggcc ctgccttggg ccgccgcgcg gtcgacgagc tcgggatgct cacgcagcgt
    14341 ccgaatggac gggccaaccg cggtcgcccc gactgtcgtc gccgcgctcc cccccaggta
    14401 gcgcgacgtg tcgccgagca gcaccgtcgg cagcatcggg gcggcgcgcc ggatccgcca
    14461 gaccgcggcc gccgagaaag acatcacgac ggcacgagag cggtcggccg acgcgggcga
    14521 ggcgataccg aaccgatgca gcagcgccag caccttgctc tccaccaacg atccgtaccg
    14581 caccggatgc ttggtctcga tgaagatctt caacggccgt cgccagtcga gcaccaggcc
    14641 caccagcgcg tccagcgtca acagcccggt gtcgccatgc ctgccgtcag gccgccagct
    14701 gttatgccat gcgccgtagt ccagcgcccg cagctgctcc agcgtcatgt cgctaaccag
    14761 gcccgtgccg gtggaggtgc ggtccacccg gcggtcgtga acgcacacca aatgcccgtc
    14821 ccgggtcagc cggacgtcgc attccacgcc ctcggcgccc tgctgcagtg caatctcgta
    14881 ggcggccagc gtgtgctcgg gccggtcacc ggacgctcct cggtgcgcca ccacgaacgg
    14941 gtgcgcgcgc ggaccgccgg cagacgtcat aggagttatg ctgccggttc ttggccttct
    15001 cgctcaaccg cggtcggcga atcggggcgg gattgttttg cgtcggggac cataacccag
    15061 cgatgcgccg gccgttcgac gggcgtgcgc tcgaacgcga acaccagccg ggccgtggcc
    15121 accactgccg ccatgcccaa caggtaggcg accacgaagc tgaccgtgtt gtcggcgatg
    15181 ccctgggtgt tgtcggggaa gctcgtcgcg gcggcgaaca tcgacgccgc gaagctgagc
    15241 agcaacagca cccaccaggc ccagatcagc cggcgcaggt gtcggtactt ctcctcggcg
    15301 gcggcgagct ccacgacgta cgtcaagggc aacgccaggt tcatcaacgg cgcgaggcat
    15361 cccacccgca gctcccatgc cgggcgcgga tccccctggt agtagtgcgc gaacgcggtg
    15421 gcgcgccggg cgagaagcca ctccgtcaac acgagatagg tgcctacgat cgcgaacatc
    15481 gcccctacgc tcgccatgac ggccacccag gtcgccagcc cggcgaccac cgagttcaac
    15541 agcacgcccc ggttcacgat cagcaacgcg tagcggacga tgtgcagaaa cgccgccacg
    15601 ccgaacaccg cgatcgtggt gatcagcatt gcgcgtaccg cccgcgacga cgggccgcgg
    15661 cgcggcggcg cggacggagt cgccgggagc tgggtggcgg gatcgacgag gccccagcgc
    15721 gggatgaaag cgtagcgagg cgtgggcccg agagcgcggc gccgccggcg cggcggcgga
    15781 ccggagccgg gtcgcaccgc gatccagcga tatccggccg gaagaagcgg cccggacctc
    15841 ggacgggacg gctgcagacc cggcgtcgtc ggggcgctgc tccactggtg cgacgtgtcg
    15901 ggcggcagca gcgcgccgcg gcagcgcgga caccactgcc gctggcggtc gcggacgttc
    15961 caccgcgtcc cgcagcggga gcacacctgg atcaccggac cagcctaagc caggattcca
    16021 ccggccgatc agatgctgcc ggtgcctccg accttgagct gacctgacgt acgtcgcgtt
    16081 ctatgccgtg gaacgctagc taccgccgtt ggtgtgacgg ttatccacaa tttccacagg
    16141 tttatccaca gccggcgtga tcagccttga ggcgctcagc gcgctgtctg gcaccgaacc
    16201 gatttcgcac tgtggataac ctgtcagcct gcagcccagg cgatcaacaa ccgtttgcta
    16261 gatttcagcg aaatgtctcg tcggtctatg tttcgcgcac ggccggaaac gcgaccccga
    16321 tgccgccgag gccgcgataa ccgtggggtt cttgcccacg ttctgctggt ggtgatcctc
    16381 ggcgtagaag aaaacacgtc tcaacggtta tgatcgcgct cgcagcgtgt ggtgtgacac
    16441 atctcacggg ggggtgcagg gttaccagtg acggcagggg gcttgtgatg gcagcgcatc
    16501 cgatcggctc gaatccggcg atgccgcctt cggagtggga gacctcctcg ccggtctacg
    16561 cgcgttcgca gatgacgtcg gcgttgcggg ccgcgttgat cgcgttggtg cttctggcga
    16621 tcctcgccct gattgtcttg ttctgaaggt tggttctccg gcctctcctg atcacaggtt
    16681 tacgtgccgc ccgtcccggg gcggaggcat ccttgcgacg cgcgcagcaa tggagcaggg
    16741 ttgaagtcgg accgaaaagg taacccgaca actgaagaac agaaaggaat ctcgtggctg
    16801 agtacacctt gcccgaattg ccgtacgact acggcgcgct cgagccccac atctcggggc
    16861 agatcaacga gctgcaccac agcaaacacc acgccaccta cgtgaagggg gccaacactg
    16921 cggtcgagca gctcgcagag gcccgggaaa aaggcgatca cgcatcaatc ttcttgcacg
    16981 agaagaacct tgccttcaat ctcggcgggc atgtgaacca ctcgatctgg tggaagaacc
    17041 tgtcccccga gggcggtgac aagccaaccg gcgaactgcc ttcggctctc gatgacgcgt
    17101 tcggctcgtt cgacaagttc caggctcagt tcaccgccgc cgccaacgga ttacagggtt
    17161 cgggctgggc ggtcctcggt tacgacagcc tcggcgacaa gctactcacc ttccagctct
    17221 acgaccagca ggccaatgtg ccactcggga tcatcccgct gctgcaggtc gacatgtggg
    17281 agcacgcgta ctacctgcag tacaagaacg tcaaggccga ctacgtgaag gctttttgga
    17341 acgtggtcaa ctgggccgac gtccaagacc gcttcgccgc ggccaccagc aagaccaagg
    17401 gcctgatctt cggctgacgc cgttttccat cggatgtgac gccagggtcg ctcgagtgcc
    17461 gaccctggcg tcacgttcgt ttgcagcctc gttggcagtc gtgtcgtatt gcgtgccagc
    17521 tgcaatttcg tcatcctgac ctcatcgacc cagtggcgcg tcgcacctgg ttcgacgtgt
    17581 ccgaagggcg gaggcaggat atgagtacgg gatccggccg ggccatcgag gtggccccct
    17641 tccattcacg cggctcgctc aagggtttcg tggtgtccgg ccggtggccg gattccacca
    17701 aggaatgggc acaactgctc atcgtcgcgg tgcgggtggc gtcgctgccc ggactgcttt
    17761 ccaccacaac gattttcggt gtccgcgagg aactgcccga ggaacccgaa cccggcaccg
    17821 tcgggctcgt cctcgccgag gggccggtcg tcggtgaatc agcgctgcct ccgggacatt
    17881 tcgccgatca cctgccccca gcgctgatga tgctgcatcc gccgtccgaa accgtgccgt
    17941 cactgccgga atgcaccggc gccgcgtcgg gatgtgtgct tctgccgggc ctaccgcatc
    18001 tggggctcga acaccgggcg gcctgggtgg aggccgaggc cgacggcacc gtcacctcga
    18061 tggtgagccg ggtgggcgtc gacccgatca gtcacccaga caccgcgatt ctggccatgc
    18121 tccttgcggc ataaggacat tcgaagccgt cgatacgggt agccgaactt tcacactgcc
    18181 tggcgaacac gctcgacact ggctatcgtc gggtgagtaa gcgaagggga gtagccccca
    18241 atcgtcgtgt cgacacactg gcgctcgcgc ccggccggcg aaccgcagac gcggtgggcg
    18301 agaccttcga ccggatgtcc ggtggccgcc atcgcggtgc cgggcgacgc gtcgaaaggt
    18361 cggatatgct caccgccacg ctcgtcagcc tcggtgtggt gttcgtcgcc gaaatcggcg
    18421 acaaatcgca gttgatcgcg atgacctatg cgctgcgcca ccgatggtgg gtggtgctgt
    18481 ccggcgtggc actcgccgcg ttcctggtgc acgggctctc ggtgaccatc ggacactttc
    18541 tcggcctgac gctgcccgaa aggccgatcg cgttcgccgc cgcgaccgcg ttcttcggat
    18601 tcgccttgtg gacctggcgg gagcgcagtg acgacggctc ggcgccgccc gcgccggagc
    18661 atcgatttgt gttgctggcc gtggtgtcgt cgtacctgct
//
LOCUS       DASWBF010000481        22381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_967853,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000481 DASWBF010000000
VERSION     DASWBF010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22381
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967853"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..713)
                     /locus_tag="VF874_17610"
     CDS             complement(102..713)
                     /locus_tag="VF874_17610"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9177805.1"
                     /translation="MFTGIVEEVGRVVEKSDLPDAVRLRIRGPVVTSDASPGDSIAVN
                     GVCLTVVQVLPGGEFTTDVMAETLNRSTLRHVDDRPEVNLERAVTVEKRLGGHIVQGH
                     VDGTGRVISRTPSQHWEVVRVALAPGLARYVVEKGSIAVDGVSLTISALGNDAGQDWF
                     EVSLIPTTLEETTLAGVPVSGEVNLEVDVIAKYVERLMQMGNR"
     gene            771..1478
                     /locus_tag="VF874_17615"
     CDS             771..1478
                     /locus_tag="VF874_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEX9177806.1"
                     /translation="MPFGRRSTAVLLAAVAVTASLVGGCSKSKDESGPPLPDAATLLK
                     QSAETTRNLKSAHVELTVDGDVAGLPVKTLSGDLTNTPAVAAKGHTRLKLAGDEVEAD
                     FVVLDSILYGSLDPGQWSDFGAASNVYDVSTILNPDTGLANILSNFTDPKADGRETIG
                     GVQTVRITGHVNEDAVHKIVPKVAPRGSVPGTAWVREDGNHELVQVKLEPSGEGNAIQ
                     MTLSKWNEPVTVEKPPI"
     gene            1478..3010
                     /locus_tag="VF874_17620"
     CDS             1478..3010
                     /locus_tag="VF874_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9177807.1"
                     /translation="MKSNRRIAITAGSLAVLLGALDTYVVITIMRDVMRDVGIALNHV
                     QKVTPIITWYLLGYVAAMPLLGRASDRFGRKLLLQSSLVGFAVGSVVTAMSGDLVTLV
                     IGRCIQGVASGALLPVTFALAADLWSQRSRAAVLGGIGAAQELGSVLGPLYGIFIVWL
                     LTHWQAVFWINVPLVAIAMVMIHFSLPSHERGEGPRERVDVVGGLLLAAALGLVVQGL
                     YNPAPDGKQVLPDWGVPVLCGALVAAVAFAIWERFARTRLIEPAGVRFAPFLCALGAS
                     VCAGAALMVTLVDVELFGQGVLGMSQNEAAGLLLRFLIALPIGALLGGWLATKVGDRA
                     IACVGLLIAAAAYWLVSDWGVDVLTTRHDLGLFTLPTFDTDLALAGLGLGLVIGPLTS
                     ATLRVVPTAQHGIASSAVVVARMIGMLVGVAALSAWGLYRFQQILHSLPKPSGGLFEI
                     RAKIAENYQTAYATEYGEIFTFTAIVCVVGAVLGLFISNRDTHDAGPAAEEEPGELAS
                     RR"
     gene            3123..3406
                     /locus_tag="VF874_17625"
                     /pseudo
     CDS             3123..3406
                     /locus_tag="VF874_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
     gene            3447..4496
                     /locus_tag="VF874_17630"
     CDS             3447..4496
                     /locus_tag="VF874_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9177808.1"
                     /translation="MLTAALRDLQWRKRRFVIAIIGTGLVFAMTLVLTGLANGFRVEA
                     QRTVDSLGLDAFLIKSGAAGPFVGSSPFPPTELERAARLPGVANAVPMVYGSATIPDR
                     GSSRNVNVFGAPDRGPGMPAVSDGRAPTNPGEIAVSSTMRRGLGQDLEIGSRTMRIVG
                     IVDDSTLLARQANVFLTVAGAQQLLFSGQPLISSIGIRGTLDRVPDGYRVVDRAGAVE
                     DLVRPLKGARTSISMMAWLLWVVAALIVGSMIYVSALERIRDFAVFKAVGVSTRSIMA
                     GLALQAVVVALLAALLGAIVSVLLGPLFPMRVDIPTLAFVLLPVIAVVIGLLASVAGL
                     RRAVTVDPAVAFGGP"
     gene            4500..5489
                     /locus_tag="VF874_17635"
     CDS             4500..5489
                     /locus_tag="VF874_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003901426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9177809.1"
                     /translation="MPDLRIKDLVVEYCRGEYAVRPIDGCDLDVSAGSLVILLGPSGC
                     GKTTLLSCLGGILSPTAGRIEFGDVDITALDRRGLSEYRRNTVGIVFQAFNLVPSLTA
                     IENVMVPMRAARKSRHDAHQRAEELLTRVGLKERMGHRPGDLSGGQQQRVAVARAIAL
                     DPPLLLADEPTAHLDFIQVEEVLKLIRELASGERVVVVATHDTRMLPLADRVVDLTPH
                     ISYSEHEPETVSIESGEVLFEQGTIGDCIYVVSEGELDIVRELADGGEELLKVAGPGD
                     YFGEMGPLFGLPRSATVRARSDAAVIGYTVQQFRERLGATGVRDIVEHREFSG"
     gene            complement(5567..5890)
                     /locus_tag="VF874_17640"
     CDS             complement(5567..5890)
                     /locus_tag="VF874_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177810.1"
                     /translation="MHDNIVDGVLAQHGGQRAKHTGDGIFATFDAPTNACLCAPESRG
                     EEWSGLAVHVGARIGAMAGPGEVLASRTVRDLSAGSGLKFESLGAHRLKGLSEDTDLY
                     RVRAR"
     gene            complement(5935..6918)
                     /gene="ribD"
                     /locus_tag="VF874_17645"
     CDS             complement(5935..6918)
                     /gene="ribD"
                     /locus_tag="VF874_17645"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX9177811.1"
                     /translation="MRLAIEHAEQVKGATYPNPPVGAVILDAAGEVVGVGGTEPTGGP
                     HAEVVALRRAGELAKGGTAVVTLEPCNHHGRTPPCVDALVAAGVSRVVYAVADPSPEA
                     AGGAKRLEQVGIDVETGVLADVVERGTLREWLHKQRTGLPHVTWKFAASVDGRSAAAD
                     GTSQWITSEAARADVHRRRAVADAIVVGTGTVFSDDPTLTARLPDGSLAQRQPLRVVV
                     GDREISTEAKVLNDDSRTMLIRTHDPHEVIKALADRTDVMLEGGPTLAGAFLRAGVVD
                     RILAYVAPILLGGPVTVIDEVGVSTIGKALRWKFDGVEQIGPDLLLSLVPG"
     gene            complement(6936..7613)
                     /gene="rpe"
                     /locus_tag="VF874_17650"
     CDS             complement(6936..7613)
                     /gene="rpe"
                     /locus_tag="VF874_17650"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9177812.1"
                     /translation="MAPPLIAPSILAADFARLADEAAAVTGADWLHVDVMDNHFVPNL
                     TIGLPVVESLLAVTDIPIDCHLMIENPERWAPPYAEAGAYNVTFHAEATDNPVAVARD
                     IRAAGAKAGLSVKPGTPLEPYLEILPEFDTLLIMSVEPGFGGQAFISEVLVKVGTARR
                     LIDSGELTILVEIDGGINDDTIEEAAAAGVDCFVAGSAVYGADDPAAAVRALRRQAAA
                     ASPHLQL"
     gene            complement(7618..8982)
                     /locus_tag="VF874_17655"
     CDS             complement(7618..8982)
                     /locus_tag="VF874_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9177813.1"
                     /translation="MTARRTRKPLDPARRAAFDVLRAVSERDAYANLALPPLLNERGI
                     TGRDAAFATELAYGASRVRGLLDAIIAKAADRSLESIDPVLLDLLRLGAYQLLRTRVD
                     AHAAVSTTVEQAGIEFDSARAGFVNAVMRTIASRDEQAWVAELAPDPVVDPVGHAAFV
                     HAHPRWIAQAFADALGRDAGELDALLASDNARPAVHLAARPGVITADELAAAVDGAVG
                     RYSPYAVYLTGGDPGRLQPVRDGQALVQDEGSQLVARALVLAELDGGDRGRWLDLCAG
                     PGGKTALLAALGKAQGARVTAVEPTPRRADMVEENTRGLDVEVLRVDGRNPEIATGFD
                     RVLVDTPCTGLGALRRRPEARWRRGPADVPALVKLQRELLASAIELTRPGGVVLYATC
                     SPHLAETVGVVADAVRRHPVEAIDTRPLFAPADRLGDSLHVQLWPHRHGTDAMFAAAL
                     RKLG"
     gene            complement(8979..9908)
                     /gene="fmt"
                     /locus_tag="VF874_17660"
     CDS             complement(8979..9908)
                     /gene="fmt"
                     /locus_tag="VF874_17660"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX9177814.1"
                     /translation="MRLVFAGTPEPALPALRRLIASPRHDVVAVLTRPDAVSGRRGKP
                     TPSPVARLAHDEGIPVLTPQRPNSDEFVELLAELAPDCCPVVAYGALLGDALLAIPPR
                     GWVNLHFSLLPAWRGAAPVQAAIAAGDTVTGATTFLIERELDSGPVYGVVTEAVRPND
                     MAGELLQRLSLSGAALLESTLDGIADGTLVPVPQPPDGVSIAPKITVDAARVRWELPA
                     QAVERRIRAVTPDPGAWTMIDCVRLKIGPVTLEDSASPLEPGELRVNRHGVRVGTGSQ
                     PVLLGQVQPPGKKPMAAADWARGARLDESARAS"
     gene            complement(9911..10447)
                     /locus_tag="VF874_17665"
     CDS             complement(9911..10447)
                     /locus_tag="VF874_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9177815.1"
                     /translation="MVTWVLLLLVVIAVAMLIVFVSGYNKVRGADVRVDEALGGIDVQ
                     LTRRASLIPSLVQTVQAFAAHEKSILDNVTAAQAALTSATHGKSVAQRGAAEKQLDTA
                     VGQVLALGQMYPNLSSSANFLDLQRNLADTEDKLAFARQYYNDAVATNNRLITTIPWM
                     FMAGAAGVREREYYQVPK"
     gene            complement(10441..12252)
                     /locus_tag="VF874_17670"
     CDS             complement(10441..12252)
                     /locus_tag="VF874_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX9177816.1"
                     /translation="MGWGRRVAALLIPLALIIIGLFWPLVLTGVSQPGTGSDPVWFSH
                     YRADYDVNADGRVEVVETINAQFPGGKHGIFEFWDVAAPHDTHVRMIPTVTAVTLDDE
                     PTPVKMEWDNSRRFLTARIGDPATYLNPGSHEFVIRSVVPNGLAPSSVGKNLAFASST
                     GEINSTPSVFYWFVVKSWNNRIDRADITVRLPARVAGVQCSTGFGRGDQCPELNLNGD
                     RIEMTMFNVAPRTPVTVRAGVDIATPPRDELPWSWKFDRILGQSVTKVLMLLGASVAA
                     GLLGYAWWRTTVESSPGFPLQYAPPQGLGPVQSEYIRTEAVPRNGLTATLFYLAERGL
                     ISLTQRGDKHWTVRGVAQPGAWADVDPVSVAVGSTLRVNQPGAEFEATNTVASGKRLS
                     AAKTDMAEAVKKWAKNEKLVVPKRAEQWVRVANLASIVLAVCGFITWGFTITLWGLPF
                     AVFFVATMRSWAEGVGSRRTPAGRELWSRVGGFHRMLTTDSAEARFDFGARKDLYTAY
                     IPFAVAAGAAALWAKKYEAATGMPAPQPDWYHSTTPWAYGAVGGSHGPSFDSFESTLS
                     SSIGAYSASQSSSSSGGGGFSGGGGGGGGGGGGGGSW"
     gene            complement(12283..14229)
                     /locus_tag="VF874_17675"
     CDS             complement(12283..14229)
                     /locus_tag="VF874_17675"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX9177817.1"
                     /translation="MLSVPHLDREFDYLVAAEQSDDAQPGVRARVRFHGRLVDAFVLE
                     RRSDTDHVGELGWLDRVVSPEPVLTPDVRRLVDAVAARYAGTRADVLRLAVPPRHARV
                     EREVREAQIPGAVGPVDVVGWSAYQSAERFLAALAEGRAARAVWQALPGEQWAGRLAE
                     AAAVVVGAGRGVLAVLPDQRDVDTLLAHSVELIDKDSVVALSAGLGPAERYRRWLAAL
                     RGNARLVIGTRSAVFAPVADLGLMIVWDDGDDTLAEPRTPYPHAREVAMLRAHQLRCA
                     AVIGGYARTAEAHALVRSGWAHDVVAARPAVRAAAPRVIALDDSGYADARDPAARTAR
                     LPSVALRAARTALDTGSPVLVQVPRRGYVPALACARCRAVTRCRHCTGPVSLPGAGST
                     GAVCRWCGRVDAALKCVRCGAEAVRAVVVGARRTAEELGRAFPGTAVVTSGGDSVVRD
                     VDGRPVLVVATPGAEPVACGGYGAALLLDGWALLGRQDLRAAEDTLRRWMAAAALVRS
                     RGDGGVVAVVADSVIPTVQALVRWDPVGHADAELEARSEVGLPPSVHIAALDGPPQAV
                     SALLERTDLPEGAELLGPVELPVAARRPASARDTDAVTRMLVRVSREHGLALAAALRR
                     AVAVTSARHDHEPVRVQIDPLHIG"
     gene            complement(14342..15130)
                     /locus_tag="VF874_17680"
     CDS             complement(14342..15130)
                     /locus_tag="VF874_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEX9177818.1"
                     /translation="MASDGALTPAPDTRTPDTPRLLAAAWVAAGWAGVAYGVFLTLTA
                     LQSEPGESLTGIFAGQSVFKALLAVLLALAAVAHPIVRERRWLMVALVFSAVGDFLLA
                     MPWWEPSFVGGLGAFLVAHLCYLGALVPLVVVTGRRLVAAAVMCALCVALLVWFWPRL
                     GPDGMTVPVTIYIAVLCAMVCAALLARLPTPWTALGAVCFAVSDAMIGIDQFILQSDV
                     LAVPIWWAYAMSQLLITAGFFFGRSVGAPAYREGDPTEPAEGNP"
     gene            15201..16697
                     /locus_tag="VF874_17685"
     CDS             15201..16697
                     /locus_tag="VF874_17685"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9177819.1"
                     /translation="MSTTTAPDYDTVIVGAGFSGIGAAVKLDEAGLHSYLIIEAGDGP
                     GGTWYWNQYPGVAVDIPSFSYQFSFEQRPDWSRTYAHGHELRAYADYCADEYGIRPKI
                     RINTKVVCAAFDEEQHLWRLDLDSGEQITARFLINAAGVLTTPKLPDIDGFDGFDGFD
                     GITIHTARWDHTQDLTGKRVAIIGTGASAVQVIPEIAPKVKHLTVFQRTPIWCFPKFD
                     VLLPKAARWAMRMPGGMALQRLASQIYVELTFTLSAQYFTVFPLAKRAEAMGKAFLRR
                     EVHDPVLREKLTPEYAVGCKRPGFHNTYLSTFNRDNVELVTDPIDKITPTGVATVEGD
                     HHDVDVLILATGFKVMDIDSLTYEIIGRGGVSMSQFWRENRLQAYEGVSLPGFPNFFT
                     VAGPYGFVGSSFFAYIETQTHHIVRCLKTAVRQGASRVEVTKEANDRYFAECMRKRHT
                     QIFWQDSCKLANSYYFDSHGDVPLRRATTLGTIWRSRRFPLDDYAFTA"
     gene            complement(16782..17984)
                     /gene="metK"
                     /locus_tag="VF874_17690"
     CDS             complement(16782..17984)
                     /gene="metK"
                     /locus_tag="VF874_17690"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9177820.1"
                     /translation="MSQSGRLFTSESVTEGHPDKICDAISDSVLDALLEQDRHSRVAV
                     ETLVTTGQVHVAGEVTTSANVDIPTIVRNRILEIGYDSSAKGFDGNSCGVNIAIGKQS
                     PDIAQGVDTAHETRVEGTGDPLDKQGAGDQGLMFGYAINDTPELMPLPIALAHRLARR
                     LTEVRKGGVLPYLRPDGKTQVTIEYEGTRAARLDTVVISTQHAADIDLDNLLAPDIRE
                     KVLNTVLDDLANETLDTSSVRLLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHG
                     GGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLADRVEVQVAYAIGKAAPVGLFVETFD
                     TETVDPAKIENAINDVFDLRPGAIIRDLDLLQPIYAQTAAYGHFGRTDIDLPWERLNK
                     VDELKSSV"
     gene            complement(18078..19319)
                     /gene="coaBC"
                     /locus_tag="VF874_17695"
     CDS             complement(18078..19319)
                     /gene="coaBC"
                     /locus_tag="VF874_17695"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX9177821.1"
                     /translation="MDRKRIIVGVSGGIAAYKACSVVRQLVEAGHSVRVIPTESALRF
                     VGAATFEALSGEPVHTDVWQDVPEVPHVQIGKQAELVVVAPATADVLARAAHGRADDL
                     LTATLLTARCPVLFAPAMHTEMWLHPATADNVATLRQRGAVVLEPASGRLTGSDTGAG
                     RLPEPEEITTFAELLLARGDAMPYDLAGCKVLVTAGGTREPIDPVRFIGNRSSGKQGY
                     ALARVAAQRGADVTLIAAHTAGLADPAGVHVVHVSSAQQLDDAVSKHAPDTHVLVMAA
                     AVADFRPTQASPSKIKKGSTPPPTIELTRTDDVLRGAVRARAEGQLPNMRAIVGFAAE
                     TGDANGTVLDHARAKLRRKGCDLLVVNAVGEGRAFEADSNDGWLLAADGAESALEHGS
                     KTLMASRIVDAIVTFLHGRTG"
     gene            complement(19400..19699)
                     /gene="rpoZ"
                     /locus_tag="VF874_17700"
     CDS             complement(19400..19699)
                     /gene="rpoZ"
                     /locus_tag="VF874_17700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX9177822.1"
                     /translation="MAPALSGVPDRAVDPSAYDTPLGITNPPIDELLERVSSKYALVI
                     YAAKRARQINDYYNQLGEGILEYVGPLVEPGLHEKPLSIALREIHADLLEHTEGE"
     gene            complement(19769..20359)
                     /gene="gmk"
                     /locus_tag="VF874_17705"
     CDS             complement(19769..20359)
                     /gene="gmk"
                     /locus_tag="VF874_17705"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX9177823.1"
                     /translation="MTNGGGPGPRVVVLSGPSAVGKSTVVRCLRDRLPDLYFSVSATT
                     RAPRPGEADGVDYHFVSPERFQRLIDEGALLEWADIHGGLHRSGTPAQPVRDATRAGR
                     PVLIEVDLAGARVIKRAMPEALTVFLAPPSWAALEARLTGRGTESPDVMARRLATAQE
                     ELAAQRDFDAVVVNSELESACEKLIALLVETAPDTA"
     gene            complement(20356..20670)
                     /gene="mihF"
                     /locus_tag="VF874_17710"
     CDS             complement(20356..20670)
                     /gene="mihF"
                     /locus_tag="VF874_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX9177824.1"
                     /translation="MALPQLTDEQRAAALEKAAAARRARAELKDRLKRGGTNLTQVLK
                     DAETNEVLGKMKVSALLEALPKVGKVKAQEIMTELEIAPTRRLRGLGDRQRKALLEKF
                     SS"
     gene            complement(21132..21956)
                     /gene="pyrF"
                     /locus_tag="VF874_17715"
     CDS             complement(21132..21956)
                     /gene="pyrF"
                     /locus_tag="VF874_17715"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9177825.1"
                     /translation="MPGFGARLADATRRRGPLCLGIDPHPELLRDWELPHDHDGLARF
                     SDICVTAFADFAVVKPQVAFFEAYGSAGYAVLERTIAALREAGVLVLADAKRGDIGST
                     MAAYAAAWAGDSPLAVDAVTASPYLGFGSLCPLLDAAAAHQRGVFVLAATSNPEGAEV
                     QRAETAGRTVAQSIVDAAAAVNRKALPEPGSVGVVVGATAQYVPDLADLGGPVLVPGI
                     GAQGGRAENLAGLGGAQPDQLLPAVSREVLRAGPDVGALRVAAERMRDAVAYLRQR"
     gene            complement(21959..>22381)
                     /gene="carB"
                     /locus_tag="VF874_17720"
     CDS             complement(21959..>22381)
                     /gene="carB"
                     /locus_tag="VF874_17720"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213590.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX9177826.1"
                     /translation="VFVSIANRDKRSLVFPVKRLADLGFRVLTTAGTAEMLRRNGIPC
                     DEVRKHFEDPSPDRPALSAVDAIRAGEVDMVINTPFGNSGPRIDGYEIRSAAVAMNIP
                     CVTTVQGASAAVQGIEAGIRGDIGVMSLQELHTTLSGP"
BASE COUNT         3453 a         7738 c         7601 g         3589 t
ORIGIN      
        1 cccttcgggc acttttcctt ggtcggtagg gcgagcacgc cgatcaccag caccacggca
       61 atcccgacca gaaccagtag tagaactctg ttcatgtgta tttacctgtt gcccatctgc
      121 ataagtcgct caacatattt cgcgatcacg tcgacttcga ggttgacctc acctgacacc
      181 ggaacgcccg ccagcgtggt ttcctcgagc gtggtgggga tcagcgacac ctcgaaccaa
      241 tcttgacctg cgtcgttacc gagagccgag atcgtcaggc tgaccccgtc gacggcaatc
      301 gagcctttct ccacgacgta acgcgccagg ccaggtgcca gcgcgacccg gacgacctcc
      361 caatgctgcg acggcgtgcg tgaaatcaca cgtccggtcc cgtcgacgtg gccctggacg
      421 atgtgcccac cgagccgctt ctcgaccgtg acggcccgct cgaggttgac ctcagggcgg
      481 tcatcgacgt gtcgcagcgt cgagcggttc agggtttcgg ccatcacgtc ggtggtgaac
      541 tcgccgcctg gcagcacctg caccaccgtc aggcacacgc cgttgaccgc gatggagtcg
      601 cctggcgacg cgtcactggt gacgacggga ccgcggattc tgagccggac agcgtcggga
      661 agatcggact tttcgacgac gcggccgact tcttcgacga tgccggtgaa caccaacaca
      721 ggctagtgcg caaacccgcc gcgtcgcccc gaccctctac gatgcaactc atgccgtttg
      781 gccgtcgaag taccgcagtt ctgctcgccg ccgtcgcagt taccgcttct cttgtcggag
      841 ggtgctcgaa gtcgaaggac gagtccgggc cgccgctgcc ggacgcggcg acgttgttga
      901 agcagtccgc ggagaccacg cgaaacctca agagcgcgca cgtcgagctg acggtcgacg
      961 gcgacgtggc tgggctaccg gtcaagacgc tctcgggtga tttgacgaac acgcctgccg
     1021 tcgcggcgaa aggccatacc cgcctcaagc tcgccggcga tgaggtggag gccgacttcg
     1081 tcgttctcga cagcatcctg tacggctcgc tggaccctgg ccagtggagc gacttcgggg
     1141 cagcctccaa cgtctatgac gtgtcgacga ttctgaatcc ggacaccggg ctggccaaca
     1201 tcctgtcgaa cttcaccgac cccaaggcgg acgggcgcga aacgatcggc ggcgtgcaga
     1261 ccgtcaggat caccggacac gtcaacgagg acgccgtaca caagatcgtc ccgaaggtcg
     1321 cgccgcgcgg atccgttccc ggcaccgcgt gggtccgcga ggacggcaat cacgaactgg
     1381 ttcaggtcaa gctggaaccc agcggcgaag gcaacgccat tcagatgacg ctgtcgaagt
     1441 ggaacgagcc ggtcaccgtc gagaagccgc cgatctgatg aagtccaacc gcagaatcgc
     1501 gatcactgcg ggcagtctcg ccgttctgct cggcgcgctg gacacgtacg tcgtgatcac
     1561 gatcatgcgc gacgtcatgc gcgacgtcgg gatcgcactg aaccatgtcc agaaggtcac
     1621 tccgatcatc acctggtatc tgctcgggta cgtcgcggcc atgccgctgc tcggccgggc
     1681 gtctgaccgg ttcggccgca agctgcttct gcagtccagc ctcgtaggct ttgcggtcgg
     1741 gtccgtcgtg accgcgatgt cgggcgatct cgtcacattg gtgatcggac gttgcatcca
     1801 aggcgtcgcc agcggcgcgc tgctgccggt cacgttcgca ctcgccgccg acctgtggtc
     1861 gcagcgcagc cgggccgcgg tgctcggcgg catcggcgcc gcacaggagc tgggcagtgt
     1921 gctgggtccg ctgtacggca ttttcatcgt ctggctgctg acccactggc aagcggtgtt
     1981 ctggatcaac gtgccgctgg tggcgatcgc gatggtgatg atccacttca gcctgccctc
     2041 acacgagcgc ggtgaggggc cgcgcgaacg ggtcgacgtg gtcggcgggc tgctgctggc
     2101 ggccgcactg ggcctggtgg tccagggcct gtacaacccg gcaccggacg gcaagcaggt
     2161 gctgcccgac tggggtgtgc cggtgttgtg cggagcgctg gtcgcggctg ttgcgttcgc
     2221 gatttgggag aggttcgccc ggacccggct gatcgagccc gcgggtgtgc ggttcgcgcc
     2281 gttcctctgc gctctgggcg cgtcggtgtg cgcgggggcg gcgctgatgg tcacgctcgt
     2341 cgacgtcgag ttgttcggcc agggcgtgtt ggggatgagc cagaacgagg cggccggcct
     2401 gctgctgcgg ttcttgatcg cgctgcccat cggagcactg ctgggtggct ggctggccac
     2461 caaggtcggt gaccgcgcga tcgcctgtgt ggggctgctg atcgccgccg ccgcgtactg
     2521 gctggtctcg gactggggcg tcgacgtgtt aaccacccgc cacgacctcg ggctgttcac
     2581 gttgccgacg ttcgacaccg acctcgcatt ggccggtctg gggctaggcc tggtgatcgg
     2641 cccgctcacc tcggcgacgc tgcgcgtcgt cccgaccgcg caacacggca tcgcatcctc
     2701 ggcggtggtg gtggcccgga tgatcggcat gctggtgggc gtcgcggcgc tgagcgcctg
     2761 ggggttgtac cggttccagc agatccttca cagccttccg aagccgtcgg gcggcctgtt
     2821 cgagatcagg gcgaagatag ccgagaacta ccaaaccgcc tatgcgacgg agtacggcga
     2881 gatcttcacg ttcaccgcga tcgtgtgcgt ggttggtgcg gtgctcgggc tgttcatcag
     2941 caaccgcgac acgcacgacg ccggccccgc ggccgaggag gagccgggcg agctcgcgtc
     3001 gcgccgctga ccgccactaa ggcacgcatt tgggggcaat tgccccatgc gaacgaccgg
     3061 acggctcggc gaagacctcc tgacgctgac acgcgacttc aggaggtctc gacggtaaga
     3121 agatcgcatt cttggtagcg ccggagggcg tcgagcaagt cgagttgacc gagccgtggg
     3181 aagcggtcaa gcaagccggc ggacgttggt cgaggctgac gtcgtgcgcg gcaggaccat
     3241 cacctggtgg ccgagcgttc agaccgatat ccgcaacgcc ggcggcaact ggggcgacga
     3301 ggaagtggtg gccgacgagc gtcgcggcaa cacgctgatc tccagccgca aacccgatga
     3361 cctgcccgct ttctgcgcca aattgctgga agcgttctcc caatagcgcc ggggatgggt
     3421 aacgtcccca ccatgcgggg gatggtgtgc tgaccgcggc gctgcgcgac cttcaatggc
     3481 gcaagcggcg attcgtcatc gccatcatcg gcaccgggct ggtgtttgcg atgacgctcg
     3541 ttctgacggg gttggccaac ggttttcgcg tcgaggccca gcgcacggtt gactcgctgg
     3601 gactcgacgc gtttctgatc aagtcgggcg cggccgggcc gtttgtggga tcgtcgccgt
     3661 tcccgcccac agaattggag agggcagcgc gcctcccggg cgtcgcgaac gcggtgccaa
     3721 tggtctatgg cagcgccacg atccccgacc gcggttcgtc gcggaacgtc aatgttttcg
     3781 gggcgccgga ccgcgggccc gggatgcccg cggtatcgga tggccgcgct ccgaccaatc
     3841 ccggcgagat cgcggtgtcg agcacgatgc gtcgaggcct cggccaggac ctcgaaatcg
     3901 gctcgcggac aatgcggatc gtaggcatcg tcgatgactc caccttgctc gcccgtcagg
     3961 ccaacgtgtt cctgacggtg gccggcgccc aacaactgct gttctcagga cagccgctga
     4021 tctcgtcgat cggcatccgc ggcacactcg accgggtgcc cgacggttat cgcgtcgtcg
     4081 accgcgcggg cgccgtcgag gacctagtgc gaccactgaa aggcgcccgc acctcgattt
     4141 caatgatggc atggctgctg tgggtggtgg ctgcgttgat cgtgggctcg atgatctacg
     4201 tgtctgcgct cgaacgcatc cgggacttcg cggtgttcaa ggcggtcggt gtttcgaccc
     4261 gctcgatcat ggccgggctg gccctgcagg ccgtcgtcgt cgctctgctc gcggcgcttc
     4321 tcggcgccat tgtttcggtt ctgctgggac cgctgttccc gatgcgggtg gacatcccca
     4381 cgttggcgtt cgtgctgctg ccggtgatcg cggtggtgat cggtcttctg gcgagcgtcg
     4441 cgggactgcg gcgcgctgtg accgtcgacc ccgcagtggc attcgggggg ccgtgaccga
     4501 tgcctgacct gcgcatcaaa gacctcgtcg tcgagtactg ccggggcgag tacgcggtgc
     4561 gtccgatcga cgggtgtgac ctcgacgtga gtgcggggtc gttggtgatt ctgctcggcc
     4621 cgagcgggtg cgggaagacg acgttgctgt cgtgcctcgg cgggatactg agcccaaccg
     4681 ccggccgtat cgaattcggc gacgtcgaca tcacggcgct ggaccggcgt ggcctatcgg
     4741 agtatcggcg caacaccgtc ggcatcgtgt tccaggcctt caacctggta cccagcctga
     4801 ccgcgatcga gaacgtgatg gtgccgatgc gcgcggcccg caagtcacgg cacgacgcac
     4861 accagcgcgc cgaagagctg ctcacccgcg tcgggctgaa agagcgcatg gggcaccgac
     4921 cgggcgatct ctccggcggc caacagcagc gtgttgcggt cgcgcgcgcg atcgcactgg
     4981 acccgccgct gctgctggcc gacgagccga ccgcccactt ggacttcatc caggtggaag
     5041 aagtgctgaa gttgatccgc gaactggcgt ccggcgagcg cgtggtggtg gtcgccaccc
     5101 acgacacccg catgctgccg ttggcggatc gcgtggtgga cttgacgccg cacatctcgt
     5161 attcggagca cgagcccgaa accgtgagca tcgaatctgg cgaggtgctg ttcgagcagg
     5221 gcacgattgg cgactgcatc tacgtggtct ccgagggtga gctcgacatc gtccgcgaat
     5281 tggccgatgg cggtgaggaa ttgctgaaag tggctgggcc gggtgactac ttcggcgaga
     5341 tgggcccgct gttcggcctg ccgcgctcgg cgaccgtgcg ggcacgctcc gacgcagccg
     5401 tcatcgggta caccgtgcag cagttccggg aacggttggg cgccacgggt gttcgcgaca
     5461 tcgttgagca cagggagttc tccggctagc ggctgtcgtc gccgagttct acgtcagggc
     5521 tgtgatctgg cccgcggaac gaccctggtg tagaactcga cgaacgctaa cgggcgcgga
     5581 cccggtaaag gtcggtgtct tccgatagcc ctttgagtcg gtgggcgcca aggctttcga
     5641 acttcaggcc ggatccggcc gacaagtcgc gcacggtgcg gctcgcgagc acctcgccgg
     5701 gcccggccat tgcgccgatc cgcgctccga cgtgcaccgc gaggccgctc cactcctcgc
     5761 cgcgcgattc cggcgcgcat aggcatgcgt tggtcggcgc gtcgaaagtc gcgaatattc
     5821 cgtcgccggt gtgcttggca cgttggccgc cgtgctgggc gagcacgccg tcgacaatgt
     5881 tgtcgtgcac gccgagttgg tggcgccagt gcgcgtcgcc acttgcactc aacatcatcc
     5941 cgggacgagg ctcagcagca gatccggtcc gatctgttcg acgccatcga acttccaccg
     6001 cagcgcttta ccgatcgtcg acaccccgac ctcgtcgatc acggtgaccg gtccgcccag
     6061 cagaatcggc gcgacgtagg cgagaattcg gtccactact cctgcgcgta agaacgcccc
     6121 cgccagcgtc gggccgccct ccagcatcac atcggtgcgg tcggcaagcg ccttgatcac
     6181 ctcgtgcggg tcgtgcgtgc ggatcagcat ggtccgcgag tcgtcgttga gaacctttgc
     6241 ctcggtggat atttcgcgat caccgacgac gacccgcagc ggctggcgct gggccaggct
     6301 gccgtcgggc aggcgcgcgg tcaatgtcgg gtcgtcggag aagaccgtgc cggtgccgac
     6361 gacgatcgcg tcggcgaccg cccgacggcg gtgcacgtcg gcgcgggccg cctcactggt
     6421 gatccactgg ctggtgccgt cggcggccgc gctgcggccg tcgacactgg cggcgaactt
     6481 ccatgtgacg tgcggcagtc cggtgcgctg cttgtgcagc cactcccgca gcgtgccgcg
     6541 ctcgacgacg tcggccagga ccccggtttc gacgtcgatg ccgacctgct cgagccgctt
     6601 ggctccgccg gccgcctccg gactcgggtc cgccaccgcg tacaccaccc gcgaaacacc
     6661 cgctgccaca agggcatcca cgcacggcgg tgtcctgccg tggtggttgc acggctcgag
     6721 ggtgaccact gcggtgccgc ccttcgcgag ctcgcccgcc cgccgcagcg ccaccacttc
     6781 ggcgtgcggg ccgcccgtcg gttcggtgcc gccgacgccg accacctcgc cggcagcgtc
     6841 gaggattacc gctcctaccg gcgggttggg gtacgtcgcg cccttgacct gctcggcgtg
     6901 ctcgatcgcc agccgcatgg cgtcgtcgta gggaatcaca gctgtagatg cggggacgcg
     6961 gccgcggcct gccggcgcag cgcccgcacg gccgcggcgg gatcgtcggc gccgtagacc
     7021 gcggagccgg cgacgaagca gtccacgccg gcggccgcgg cttcctcgat ggtgtcgtcg
     7081 ttgatcccgc cgtcgatctc gacgaggatc gtcaactcgc ccgagtcgat gaggcggcgc
     7141 gcggtaccga ccttgacgag cacttccgag atgaacgcct gaccgccgaa tcccggctcg
     7201 acggacatga tcagcagcgt gtcgaactcg ggcaggatct ccaggtacgg ctcgagcggt
     7261 gtgccgggtt tgaccgacag cccggccttg gctccggcgg cacggatgtc gcgggcgacg
     7321 gcgacggggt tgtcggtggc ctcggcgtgg aacgtgacgt tgtacgcgcc cgcttcggcg
     7381 tacggcgggg cccagcgctc ggggttctcg atcatcagat ggcagtcgat agggatgtcg
     7441 gtgacggcga gcaggctttc gacgaccggc aggccgatcg tcaggttcgg cacgaagtgg
     7501 ttgtccatca cgtcgacgtg cagccagtcc gcgccggtga cggccgcggc ttcgtcggcc
     7561 aggcgggcga agtcggcggc gaggatggac ggcgcgatca gcggtggagc catgggttca
     7621 ccctaatttg cgcagcgctg ccgcgaacat cgcgtcggtg ccgtgccggt gcggccagag
     7681 ctgaacgtgc aggctgtcgc cgagccggtc ggccggcgcg aacaacgggc gggtgtcgat
     7741 cgcctcgacc gggtggcggc gcaccgcgtc ggcgacgacg ccgacggttt cggcaaggtg
     7801 cggcgagcag gtcgcgtaca gcaccacgcc gccgggccgc gtcagctcga tcgccgatgc
     7861 cagcagctcg cgctgcaact tgaccagcgc cggcacatcg gccgggccgc gacgccagcg
     7921 ggcctccgga cggcgccgca gcgcgcccag cccggtgcag ggggtgtcga cgagaacgcg
     7981 gtcgaaaccc gttgctattt cagggtttcg gccgtcaacc cgcagcactt ccacgtccag
     8041 ccctcgggtg ttttcttcga ccatgtccgc gcggcgcggc gtgggttcga cggcggtgac
     8101 acgcgctccc tgtgcttttc ccagcgcggc cagcagcgcg gtcttgccgc caggccctgc
     8161 acacaggtcc agccatcggc cgcggtcgcc gccgtcgagc tctgccagca ccagcgcgcg
     8221 ggccaccagc tggctgccct cgtcctggac cagcgcctgc ccgtcgcgca ccggctgcag
     8281 ccggccgggg tcgccgccgg tgaggtacac cgcgtacggc gaataccgcc cgaccgcgcc
     8341 gtccaccgca gcggccagct cgtcggcggt gatcacgccg gggcgcgccg ccaagtgcac
     8401 tgccgggcgg gcgttgtcgc tcgccagcag cgcgtccagc tcgcccgcgt cccggcccag
     8461 cgcatcggcg aacgcctgcg cgatccaccg cggatgggca tgcacgaacg cggcgtgccc
     8521 gaccgggtcg actaccgggt cgggcgccag ctccgcgacc cacgcctgct cgtcgcgcga
     8581 tgcgatcgtc cgcatcacgg cattgacgaa accggctcgg gcggaatcga attcaatgcc
     8641 ggcctgctcg acggtggtgg acaccgccgc gtgcgcgtcc acccgcgtgc gcagcagttg
     8701 gtacgcgccg agccgtagca ggtccagcag caccgggtcg atgctttcga gtgaccggtc
     8761 ggcggccttc gcgatgatcg cgtcgagcag cccccgaacg cgggacgcgc cgtaggccag
     8821 ttcggtggcg aaggccgcgt cgcggccggt gatgccgcgc tcgttgagca gcgggggcag
     8881 cgcgaggttg gcgtaggcgt cgcgctcgga caccgcgcgc agcacgtcga atgccgcccg
     8941 gcgggccggg tcgagcggct tacgcgtccg acgagcggtc acgacgcgcg cgcggactcg
     9001 tcgagacgtg cgccgcgggc ccagtctgcg gcggccatcg gcttcttgcc gggcggctgg
     9061 acctgtccga gcagaaccgg ctgcgaaccg gtcccgacgc gcacgccgtg ccggttcacc
     9121 cgcagctccc ccggctccag cggactggcg gagtcttcca acgtgaccgg gccgatcttc
     9181 agccggacgc agtcgatcat cgtccaggcg cccgggtctg gcgtgaccgc gcggatgcgc
     9241 cgttcgaccg cctgcgcggg cagctcccag cgcacccggg ccgcgtcgac ggtgatcttc
     9301 ggcgcgatac tcaccccgtc tgggggttgc ggcaccggga ccagcgtgcc gtcggcgatg
     9361 ccgtccagcg tcgactccag cagagccgca cccgacagcg aaagccgctg cagcagttca
     9421 cctgccatgt cattcgggcg gacggcttct gtgacgactc cgtagaccgg gccgctgtcc
     9481 agttcgcgtt cgatcaggaa agtcgtcgcg cccgtcacgg tgtcgccggc cgcgatcgcc
     9541 gcctgcaccg gggcggcgcc ccgccacgcg ggcagcagcg agaagtgcag gttcacccag
     9601 ccgcgcggcg ggatcgccag cagcgcgtcc cccagcagtg cgccgtaggc gaccacaggg
     9661 cagcagtcgg gggccagctc ggccagcagc tcgacgaact cgtcggagtt cggccgctgc
     9721 ggcgtcagca ccgggatgcc ctcgtcgtgg gccagccgtg ccaccggcga gggggttggc
     9781 ttgccgcgcc gacccgacac cgcgtcgggg cgggtcagca ctgcgaccac gtcgtggcgg
     9841 ggcgacgcga tcaggcgccg caacgccggc agcgcgggtt ccggcgttcc ggcgaagacg
     9901 agtcgcatgc ctacttcggc acctggtagt actcgcgctc acgcaccccc gccgcaccgg
     9961 ccatgaacat ccacgggatc gtcgtgatga gccggttgtt ggtggccacc gcgtcgttgt
    10021 agtactgccg tgcgaacgcc agcttgtcct cagtgtcggc cagattgcgt tgcaggtcaa
    10081 ggaaattcgc ggacgaactc aggttcgggt acatctgacc cagtgccagc acctggccga
    10141 ccgcggtgtc gagctgcttc tcggccgcgc cgcgctgcgc caccgacttg ccgtgggtgg
    10201 cgctggtgag tgcggcttgc gccgcggtga cgttgtcgag gatcgacttc tcgtgggcag
    10261 cgaacgcctg cacggtctgg accaggctgg ggatcagcga cgcccggcgg gtcagctgga
    10321 cgtcgatgcc gccgagtgcc tcgtcgacgc ggacgtcggc gccgcgaact ttgttgtagc
    10381 ccgacacgaa cacgatgagc atcgcgacgg cgatcaccac cagcagcaac agaacccatg
    10441 tcaccaggaa ccgcctcctc ctcctccacc gccgccaccg ccgcccccgc cgctgaagcc
    10501 accgccacca ctggacgacg acgactgcga cgcggaatac gcgccgatcg acgacgacaa
    10561 cgtcgactcg aagctgtcga agctcggccc gtgcgagccg ccgacggcgc cgtacgccca
    10621 cggtgtggtg ctgtgatacc aatccggctg cggcgcaggc attcccgtcg ccgcctcata
    10681 cttcttggcc cacagcgccg cggcgccggc ggccaccgcg aacggaatgt aggcggtgta
    10741 gaggtccttg cgggcaccga agtcgaaacg cgcctccgcc gaatcggtgg tcagcatccg
    10801 gtggaatccg cctacgcgcg accacaactc gcggccggcg ggggtgcgcc ggctgccgac
    10861 gccttcggcc caggagcgca tggtggccac gaagaacacc gcgaacggca ggccccacag
    10921 cgtgatcgtg aagccccatg tgatgaagcc gcacaccgcg agcacgatgg acgccaggtt
    10981 ggcgactcgc acccactgtt cggcgcgctt cggcaccacc agcttctcgt tcttcgccca
    11041 cttcttgacg gcctcggcca tgtcggtttt cgccgcggac aacctttttc cggacgcgac
    11101 ggtgttcgtc gcctcgaact ccgcaccggg ctggttcacc cgcagcgtgg atccgactgc
    11161 gacgctgacc gggtcgacat cggcccatgc gccgggctgc gcgacacccc gaaccgtcca
    11221 gtgcttgtcg ccgcgctggg tcagcgagat taggcctcgt tcggcgagat agaacagcgt
    11281 ggcggtaagg ccgttgcggg gaacggcttc ggtgcggatg tactcgctct gcacaggccc
    11341 gagaccttgg ggcggtgcgt actgcagcgg aaagccgggg ctgctctcca ccgtcgtgcg
    11401 ccaccacgcg taccccagca gcccggcggc gacgctcgcg ccgagcagca tcagcacctt
    11461 ggtcaccgac tggcccagaa tgcggtcgaa cttccaggac cagggcagct cgtcgcgggg
    11521 cggggtggcg atgtccacgc ctgcgcgcac cgtcaccggc gtgcggggcg cgacgttgaa
    11581 catcgtcatc tcgatccggt cgccgttgag gttcaactcc gggcactggt cgcctctgcc
    11641 gaacccggtg gagcactgaa cgccggccac ccgagcgggc agcctgaccg tgatgtctgc
    11701 gcggtcgatc cggttgttcc agctcttgac gacgaaccag tagaaaaccg aaggggtgct
    11761 gttgatttcg cctgtcgacg acgcgaacgc caggttcttg cccacgctgg acggcgcgag
    11821 cccgtttggg acaaccgagc ggatcacgaa ttcgtggctg cccgggttga gatacgtggc
    11881 ggggtcgcca attctcgcgg tgaggaatcg gcggctgttg tcccactcca tcttgaccgg
    11941 ggtgggttcg tcgtcgagcg tcaccgctgt caccgtcggg atcatccgga cgtgggtatc
    12001 gtgcggcgcg gcgacgtccc agaattcgaa gatgccgtgc ttgccgcccg ggaactgcgc
    12061 attgatcgtc tcgacaacct cgacgcggcc gtcggcgttg acgtcgtagt cggcccggta
    12121 gtgcgagaac cacaccgggt ccgacccggt gcccggctgg gacactccgg tgagcaccag
    12181 cggccagaac agtccgatga tgatgagcgc caacgggatt aacagcgccg cgacacggcg
    12241 accccaaccc atcggcatcc tcctcggtct gttcccggct actcacccta tgtgcaacgg
    12301 gtcgatttgg acgcgaaccg gctcatggtc atggcgcgcg gacgtcaccg cgaccgcgcg
    12361 gcgaagcgcc gcggccagcg ccaacccgtg ctcgcggctc acccgaacca gcatccgcgt
    12421 caccgcatcg gtgtccctcg cactcgccgg ccgacgcgcg gccaccggca actcgaccgg
    12481 tccgagcagt tcagccccct cgggcaggtc ggtgcgctcg agcagtgcgc tgaccgcctg
    12541 cggcggaccg tcgagcgcgg cgatgtgcac gctcggcggc agcccgactt cggatctggc
    12601 ctccagttcc gcgtcggcgt gaccgaccgg atcccagcga accagcgcct gaacggtcgg
    12661 gatcaccgaa tcggccacca ccgccacgac gccgccgtcg ccgcggctgc gaaccagcgc
    12721 cgctgcagcc atccagcgcc gaagcgtgtc ctcggcggcc cgtaagtcct gccgccccag
    12781 cagcgcccag ccgtccagga gcagcgcagc gccgtatccg ccgcacgcca ccggttcggc
    12841 ccccggcgtc gcgaccacga gcaccgggcg cccgtcgacg tcgcggacca cgctgtcacc
    12901 gccggacgtg acgaccgcgg tgccggggaa cgcgcggccc agctcctcgg ccgtgcgtcg
    12961 ggcgccgacg acgaccgcgc gcaccgcctc ggcgccgcag cgcacgcact tcaatgccgc
    13021 gtcgaccctg ccgcaccacc ggcacaccgc gccggtggaa ccggcgccgg gcagcgacac
    13081 cgggcccgtg cagtgccggc agcgggtgac ggcacggcag cgcgcgcagg ccaacgcggg
    13141 cacgtagccg cggcgcggca cctgcaccag caccggcgaa ccggtgtcta gtgccgttcg
    13201 ggcggccctc aacgcgacgg acggcagccg ggcggtgcgc gcggcaggat cccgtgcgtc
    13261 ggcgtagccg ctgtcgtcca gcgcgatcac gcgcggtgcc gccgcgcgca ccgcgggccg
    13321 ggcggccacc acgtcgtgtg cccatccgct gcgcaccagc gcgtgcgcct ccgcggtgcg
    13381 cgcgtagccg ccgatcaccg ccgcgcaccg cagctgatgg gcgcgcagca tcgcgacctc
    13441 gcgggcgtgc ggatacggcg tgcggggctc ggcgagcgtg tcgtcgccgt cgtcccagac
    13501 gatcatcagc ccgaggtcgg cgacgggcgc gaacaccgcg ctgcgggtgc cgatcaccag
    13561 ccgggcgttg ccgcgcaggg cggccaacca gcggcggtac cgttcggccg gccccagacc
    13621 cgccgacaac gcgaccaccg agtctttgtc gatcagctcg acgctgtgcg ccagcagcgt
    13681 gtcgacgtcg cgctggtcgg gcagcaccgc cagtacgccg cggccggcgc cgacgacgac
    13741 ggccgccgct tcggccagcc gcccggccca ctgctcgccg ggcagcgcct gccacaccgc
    13801 gcgggcggcg cggccctcgg cgagcgcggc caggaatcgc tcggcgctct ggtaggcgct
    13861 ccagccgacg acgtcgaccg gccctacggc tccggggatc tgcgcctcgc gcacctcacg
    13921 ctcgacgcgg gcatgacgag ggggcaccgc gagccgcagc acgtcggcgc gcgtgccggc
    13981 ataccgcgcc gcgacggcgt cgaccagacg ccggacgtct ggtgtcagta ccggctccgg
    14041 cgagacgacg cgatccagcc acccgagttc gccgacgtga tcggtgtcgg accgtcgctc
    14101 caacacgaac gcgtcgacga ggcggccgtg gaaccgcacc cgggcccgga cgccgggctg
    14161 cgcgtcgtcg gactgctcgg ccgcgacgag gtagtcgaat tcgcggtcca ggtgcggcac
    14221 cgacagcatc ggcagcagcc gggcgatcgg ctcgtggtcg gcggcccgcc ggctcgtgct
    14281 tatgtcgcgc ccttggcccg ccaccgatcg gcatcgatct gggcgagcgc gagctcgtcg
    14341 gtcatgggtt cccttcggca ggttcggtgg gatcgccctc acggtaggcc ggggcaccga
    14401 cagagcgccc gaagaagaaa cccgcggtga tgagcagctg agacatcgcg tatgcccacc
    14461 agatcggcac cgcgagcacg tcggactgca ggatgaactg gtcgatgccg atcatcgcgt
    14521 ccgacaccgc aaaacacacc gcgcccagcg ccgtccacgg cgtcggaagc cgcgccagca
    14581 gcgccgcgca caccatcgcg cacagcaccg cgatgtagat cgtgactggc accgtcatcc
    14641 cgtccggacc cagcctcggc cagaaccaga ccagcagtgc cacgcacagc gcgcacatga
    14701 cggccgcggc caccagccgc cgacctgtca cgacgaccag cggcacgagt gcgccgaggt
    14761 agcacagatg cgccaccaga aacgcgccca gcccgccgac gaacgacggt tcccaccacg
    14821 gcatggccag taggaagtcg ccgaccgccg agaacaccag cgccaccatc agccagcgcc
    14881 gctcccgcac aatcgggtgt gcgaccgcgg ccagggccag cagcaccgcc agcagtgctt
    14941 tgaacaccga ctgcccggcg aagatacccg tcagcgactc gccgggctct gactgcagcg
    15001 cggtgagcgt caagaacacg ccgtaggcca cgcccgccca tccggcggcg acccaagccg
    15061 cggcgagcaa ccgcggtgtg tccggtgtcc gcgtgtcagg ggctggggtc aaggccccat
    15121 cggatgccat cctgcagaag ctactcgacg cggtagaggg agccgtacca ctgacaccgc
    15181 ggcaatgctt tgctagatca atgagcacca cgacggcacc cgactacgac acggtgatcg
    15241 tcggcgcagg attctccgga atcggcgccg cggtaaagct cgacgaggcc gggctgcatt
    15301 cgtacctcat catcgaggcc ggtgacggcc cgggcggcac ctggtattgg aatcagtatc
    15361 ccggtgtggc cgttgacatt ccgtcgttct cgtaccagtt ctcgttcgag cagcgaccgg
    15421 actggtcccg cacgtacgca cacggacacg aattgcgggc ctatgccgac tactgcgcgg
    15481 acgaatacgg aatccgtccg aagatccgta tcaacaccaa ggtggtctgc gccgcgttcg
    15541 acgaggaaca acacctgtgg cggctggatc tcgactccgg agaacagatc accgcgcgat
    15601 ttctgatcaa cgccgccggg gtgctcacca cgcccaagct tcccgacatc gacggcttcg
    15661 acggcttcga cggcttcgac ggcatcacca tccacaccgc gcgctgggat cacacccagg
    15721 atctgaccgg caagcgtgtc gcgatcatcg gcaccggcgc gtcagccgtc caggtcatcc
    15781 ccgagatcgc tccgaaggtc aagcacctca cggtgtttca gcgcacgccc atctggtgtt
    15841 tccccaagtt cgacgtgctg ctgcccaagg cggcccgctg ggcgatgcgg atgcccggcg
    15901 gcatggcatt gcagcggctg gcgagccaga tctacgtcga gttgacgttc acattgtcag
    15961 cgcagtactt cacggtgttc ccgttggcca agcgcgctga agccatgggg aaggcgttct
    16021 tgcgccgcga ggtgcacgac ccggtccttc gcgagaagct cacgcccgag tacgccgtcg
    16081 gctgcaaacg gcccgggttt cacaacacct atctgtcgac gttcaaccgc gacaacgtcg
    16141 agctggtcac cgatcccatt gacaagatca ccccgacggg agtggcgacg gtcgaaggcg
    16201 accatcacga cgtcgacgtg ctgatcctgg cgaccgggtt caaggtgatg gacatcgaca
    16261 gcctcaccta cgagatcatc gggcgcggcg gggtgtcgat gagccagttc tggagagaga
    16321 accgactgca ggcctacgaa ggtgtcagcc tgcccggctt cccgaacttc ttcacggtgg
    16381 ccggaccgta cggcttcgtc gggtcgtcgt ttttcgcata catcgagacg cagacccacc
    16441 acatcgtgcg gtgcctgaaa actgctgtgc gacagggagc ttcgcgcgtc gaggtgacga
    16501 aagaggccaa cgaccgttat ttcgccgaat gcatgcgcaa gcgtcacaca cagatcttct
    16561 ggcaggacag ctgcaagctg gcgaacagct actacttcga cagccacggc gacgtgccgc
    16621 tgcgccgggc caccacgcta gggacgatct ggcgcagccg ccggttcccg ctcgacgact
    16681 acgcctttac tgcctgaccg tcgagatcga cgtttcgcag gagaagtgcg agtagacatc
    16741 tgcaaaacgt cgatttcgcg cgcagaacta ggcggcagga actacaccga ggacttcagc
    16801 tcgtcgacct tgttcagccg ctcccagggc aggtcgatat cggtgcggcc gaagtgcccg
    16861 tacgccgcgg tctgcgcgta gatcggctgc agcaggtcca ggtcgcggat gatcgcgccg
    16921 ggccgcaggt cgaacacgtc gttgatcgcg ttctcgatct tggcggggtc gacggtctcg
    16981 gtgtcgaacg tctcgacgaa cagcccgacc ggcgctgctt tgccgatcgc gtaggcgacc
    17041 tgcacctcga cccggtcggc caagcccgcg gcaacgacgt tctttgccac ccagcgcatc
    17101 gcgtacgccg cggaacggtc caccttggac ggatccttgc cggagaacgc cccgccgccg
    17161 tggcgggccc atccgccgta ggtgtcgacg atgatcttgc ggccggtcag gccggcatca
    17221 cccatcgggc cgcccagcac gaacttgccg gtcgggttga ccagcagccg taccgacgac
    17281 gtgtcgagcg tctcgttcgc gaggtcgtcg agcacagtgt tgagcacctt ttcccggatg
    17341 tcgggcgcga gcaggttgtc caggtcgatg tcggcggcat gctgggtgga gatcacgacc
    17401 gtgtccagcc gcgccgccct cgtgccctcg tactcgatgg tgacctgcgt cttgccgtcc
    17461 ggacgcaggt acggcagcac gccgcccttg cggacctcgg tgagtcggcg ggccagccgg
    17521 tgggcgagtg cgatcggcag cggcatcaac tccggcgtgt cgttgatcgc atagccgaac
    17581 atcaggccct ggtcgccggc gccctgcttg tccagcggat caccggtacc ctcgacccgc
    17641 gtctcgtggg cggtgtctac gccttgggcg atgtcgggcg actgcttgcc gatcgcgatg
    17701 ttgaccccgc acgagttgcc gtcgaagccc ttggccgagg agtcgtagcc gatctcgagg
    17761 atgcgattcc gcacgatcgt cgggatgtcg acgttcgccg aggtggtcac ctcgccggcg
    17821 acgtgcacct ggccggtggt gaccagtgtc tccaccgcca cccgcgagtg ccgatcctgc
    17881 tccagcagcg cgtcgagcac cgagtcgctg atcgcgtcac agatcttgtc gggatgacct
    17941 tcggtcaccg actcgctggt gaacagccga cctgattggc tcacggttcg tcctctcgat
    18001 gctcgaaact tagttcatca aataatatgc tttgcctccg ttggcaagga tttcgcgcag
    18061 ccagttcgcg gccggggcta cccggtgcgg ccgtgcagga acgtcacgat cgcgtccacg
    18121 atacgactgg ccatcaacgt cttgctgccg tgctccaagg ccgactcggc cccgtcggcg
    18181 gccagtagcc agccgtcgtt gctgtccgcc tcgaacgcgc ggccctcgcc gacggcgttg
    18241 acgacgagca ggtcacagcc cttacgacgc agcttggcgc gggcgtggtc gaggacggtg
    18301 ccattggcgt cgccggtttc ggcggcgaag ccgacgatcg cgcgcatgtt cggcaactga
    18361 ccctcggcgc gcgcccgcac ggcgccgcgc agcacgtcgt cggtacgggt cagctcgatc
    18421 gtcggcggtg gcgtgctgcc cttcttgatc ttgctgggtg acgcctgcgt gggccggaag
    18481 tcggcgactg cggcagccat caccagcacg tgcgtgtcgg gcgcatgctt ggacaccgcg
    18541 tcgtccagct gttgtgccga gctgacgtgc acgacgtgga cgccggccgg gtcggccaac
    18601 ccggcggtgt gggcggcgat cagcgtcaca tcagcgcctc gctgcgccgc tacccgggcc
    18661 agcgcatacc cctgcttgcc ggagctgcgg ttaccgatga agcgcacggg gtcgatcggc
    18721 tcgcgggtgc cgccagcggt gaccagcacc ttgcagccgg ccaagtcgta aggcatcgcg
    18781 tcgccgcgcg cgaggagcaa ctcggcgaac gtcgtgatct cctcgggctc gggcagccgt
    18841 ccggcgccgg tgtcggatcc ggtgagccgg cccgacgcgg gttccaggac gacggctccg
    18901 cgctgccgca gcgtggcgac gttgtcggcc gtggccgggt gcagccacat ctcggtgtgc
    18961 atcgccggcg cgaacagcac cggacatcgg gctgtcagca gcgtggccgt cagcaggtcg
    19021 tcggcgcggc cgtgcgcggc acgcgccaac acgtcggccg tggcaggcgc gaccacgacc
    19081 agctcggcct gcttgccgat ctgcacatgc ggcacctcgg gcacgtcctg ccagacgtcg
    19141 gtgtgcaccg gttcaccgga cagcgcctcg aacgtggcgg cgccgacgaa gcgcagcgcg
    19201 gactcggtgg ggatcacccg gacgctgtgg ccggcttcga cgagctgtcg gaccaccgag
    19261 caggccttat aggcggcgat tccaccggaa acgccgacga tgatccgctt gcggtccatt
    19321 ctcagcccgt ctgttcttct cccgagaggc tctgggcctt ctgctcttcg cccgagaggc
    19381 tcatcgctgc gccccggcct tattcgccct cggtgtgctc gagcaggtcg gcgtggatct
    19441 cgcgcagcgc gatcgacagc ggcttctcat gcaggcccgg ctcgaccagc ggcccgacgt
    19501 actcgaggat gccttcgccg agctggttgt agtagtcgtt gatttggcgg gcgcgcttgg
    19561 cggcgtaaat gaccagcgcg tacttgctcg aaacccgctc cagtagctcg tcaatgggcg
    19621 ggttggtgat gcccagcggc gtgtcgtagg cgctcgggtc cactgcccgg tcaggcactc
    19681 cggacagtgc gggggccagc gccgagtcgt gcgaaatgct cacgaaagat tgtctcctga
    19741 gagtgaaaga tctgggttct gcgggtgatc acgccgtgtc cggcgcggtt tccacaagta
    19801 aggctatcaa tttttcgcac gcggactcca attcgctgtt gacgacgacc gcgtcgaagt
    19861 cgcgctgagc tgccagttcc tcctgagcgg tggccagccg gcgcgccatc acgtccgggc
    19921 tctcggtgcc ccgaccggtg agccgtgcct ccagcgccgc ccagctcggc ggcgccagga
    19981 acaccgtcaa cgcctcgggc atggcccgct tgatgacccg ggcgccggcg aggtcgacct
    20041 cgatcagcac tgggcgaccg gcgcgggtcg catcccgtac cggctgagcc ggcgttcccg
    20101 agcggtgcag gccgccgtgg atgtcggccc actccagcag cgcgccctcg tcgatcaggc
    20161 gctggaacct ctcagggctg acgaagtggt agtcgacgcc gtccgcctcg ccgggccgcg
    20221 gtgcccgcgt cgtcgccgac acgctgaagt acaggtcggg caagcggtcc cgcaggcagc
    20281 ggacaaccgt ggacttcccg acggccgagg gaccggacag cactacgacg cgcggacccg
    20341 gtcccccgcc attcgtcagg agctgaattt ttccagcagg gccttccgct gacggtcgcc
    20401 cagtccgcgc agtcggcggg tgggggcgat ctccagctcg gtcatgattt cctgtgcctt
    20461 gaccttgcca actttgggca gtgcctcgag cagcgcggaa accttcatct tgccgaggac
    20521 ctcgttggtc tcggcgtcct tgagcacctg cgtgaggttg gtgccgcctc gcttgagccg
    20581 atccttgagt tcggcccgcg cccgacgagc ggcagcagcc ttctccaacg cagccgcgcg
    20641 ctgctcgtca gtcaactggg gaagggccac gattcctccg tctcatcacc atcgattttt
    20701 cgtgcggccg gagtaaacca gccagcgacg acgaccgtac ccacgctgtc cgacgattgc
    20761 gaaccccacc ccctggtttt ggcggcaaaa tgccagcgtg tcgcgccgac acgacccgtc
    20821 cgagcgcttc ggatcggtgc tgtcctgcac tggccgcgga gtcgttggca actgcggcga
    20881 gagactattt tagcagttca aagcaaattc taccggtggc tgagcaaccc tcgactcgct
    20941 gtgcgggaca gccttcgtag cgtcgattgc cgcgctgagc gtaaaagtgc aggaaattgc
    21001 ccgttcgtgg cagccgtggg cgtgtcgcgc ccgcgttcgt cgccgaaaca gaacctacgg
    21061 ctgcgatccg tgcggaagca caacccaggc ttctgtctcg ctgtcgggcg ctgctgcgac
    21121 gtcgtatggg atcaccgctg ccgcagatac gcgactgcgt cgcgcatccg ttccgcggcc
    21181 actcttagtg cgccgacgtc gggaccggcc cgcagcacct cgcgcgacac cgcgggcagc
    21241 agctgatccg gctgtgcgcc accgagacca gccaggtttt cggcacgacc gccctgcgcc
    21301 ccgattcccg gcacgagcac cggcccgccg aggtcggcga ggtcggggac gtactgcgcg
    21361 gtggcgccga ccaccacacc gacggagcct ggctcgggca gcgctttgcg gttgacggcc
    21421 gcggcggcgt ccacgatcga ctgcgccacc gtgcgccctg cggtctcggc ccgctggacc
    21481 tcggcgccct ccggattcga cgtcgcagcc agcacgaaca cgccgcgctg gtgggcggcg
    21541 gcggcgtcca gcagggggca cagcgacccg aagccgaggt acggcgacgc ggtgacggcg
    21601 tcgacggcca gcggcgagtc gcccgcccag gcggcggcgt aggcggccat cgtcgagccg
    21661 atgtcgcctc gtttggcgtc ggcgagcacg agcacgccgg cctcgcgcag cgccgcgatc
    21721 gtgcgctcca gcacggcgta tccggcggat ccgtacgcct cgaagaacgc cacctgcggt
    21781 ttgacgaccg cgaagtcggc gaacgcagtc acgcagatgt cgctgaatcg ggcgagcccg
    21841 tcgtggtcat gcggtagctc ccagtcgcgg agcagttcgg ggtggggatc gatgcccagg
    21901 cagagcggcc cgcgtcgtcg ggttgcgtcg gccaacctgg cgccgaatcc cggcaccgct
    21961 acggcccgct cagcgtggtg tgcagttcct gcagcgacat caccccgatg tcaccgcgga
    22021 tgccggcctc gatgccctgg accgcagccg acgcaccctg caccgtcgtc acgcagggga
    22081 tgttcatcgc cactgccgcg gaccgaattt cgtagccgtc gatgcgcggc ccggaattgc
    22141 cgaacggcgt gttgatgacc atgtcgacct cgccggccct gatcgcgtcc accgccgaca
    22201 gcgcgggccg gtcggggctc gggtcttcga aatgcttgcg cacctcgtcg cacggaattc
    22261 cgttgcggcg caacatctcc gccgttcccg cggttgtcag cacgcggaat cccaggtctg
    22321 ccagccgctt cacggggaac accagtgatc gcttgtcgcg gttggctatc gacacgaaca
    22381 c
//
LOCUS       DASWBF010000482        18786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_377575,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000482 DASWBF010000000
VERSION     DASWBF010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18786
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377575"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /gene="atpA"
                     /locus_tag="VF874_17725"
     CDS             complement(<1..1089)
                     /gene="atpA"
                     /locus_tag="VF874_17725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX9177827.1"
                     /translation="MAELTISPDDIQGAIEEYVSSFTADTAREEVGTVIDTGDGIAHV
                     EGLPSVMTQELLEFEGGVLGVALNLDEHNVGAVILGNFEQIAEGQQVKRTGEVLSVPV
                     GDAFLGRVVNPLGQPIDGRGDIETDERRALEIQAPSVVQRQGVKEPLQTGIKAIDAQT
                     PIGRGQRQLIIGDRKTGKTSICVDTILNQRQNWESGDEKQQVRCVYVAIGQKGTTIAS
                     VHNALEEGGAMDYTTIVAAPASDSAGFKWLAPYTGSAIGQHWMYDGKHVLIVFDDLSK
                     QAEAYRAISLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDEMGGGSLTGLPIIETKA
                     NDISAYIPTNVISITDGQCFLESDLFNQG"
     gene            complement(1133..2470)
                     /locus_tag="VF874_17730"
     CDS             complement(1133..2470)
                     /locus_tag="VF874_17730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B/delta"
                     /protein_id="HEX9177828.1"
                     /translation="MSTFIGQLVGFAVIAFLVWRYVVPPVRRLMANRQDAVRKQLEDS
                     AAAEKRLVEAKQAHEKALQEAEAEAKRVIEEARADAKSIAELLRVQADAEVERVKVQG
                     QQHIQLLRAQFVRQLRQDLGSEAVRRAGGLVRDHVSDSDAQAATVDRFLDGLDEMAPS
                     SMTIEDPATAKLRSASREALNTLIERFDEVTGDLNVGQLETLADELASVTKLFNRETI
                     LTRHLADPSNDPEPKARLLESVLGGQVGDSALDILKAAVSGRWSSDSNLVDAVEHVAR
                     LALMVRAERGHHAEEVEEQLFWFGRILDAEPQLGTLLSDHTRPADGRVWLLENVLNRS
                     QGVNPTTAALLKQTVDLLRGGRADEAVAALAQLAVARRDEVIAHAKAAAELSDAQRNR
                     LTEVLSRIYSRPVSVQLEVDPKLLGGLTVSIGDEVIDGALSSRLAEARNRLPD"
     gene            complement(2475..2981)
                     /locus_tag="VF874_17735"
     CDS             complement(2475..2981)
                     /locus_tag="VF874_17735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX9177829.1"
                     /translation="MGEVTMTFVAEEGGQSNFLVPNGTFFVVLIIFLIVLGVIGTFVV
                     PPVMRVLHERDEMVTKTMENGRQADKQFAAADADYRAEMARARREASGVRDEARSEGR
                     KILEDMKGRASEQAALAMQKATEELKQQSDAISADLRASMETLAATLASRVLGVDVGQ
                     EAAAKTGR"
     gene            complement(2991..3236)
                     /locus_tag="VF874_17740"
     CDS             complement(2991..3236)
                     /locus_tag="VF874_17740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX9177830.1"
                     /translation="MDPTIAAGALIGGGLIMAGGAIGAGIGDGIAGNALIAGIARQPE
                     AQGRLFTPFFITVGLVEAAYFINLAFMALFVFATPVK"
     gene            complement(3305..4057)
                     /gene="atpB"
                     /locus_tag="VF874_17745"
     CDS             complement(3305..4057)
                     /gene="atpB"
                     /locus_tag="VF874_17745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9177831.1"
                     /translation="MTETLLAEGAIEVGHHETAKWFGLTVNTDTILATAIAAVIVIAL
                     ALFLRAKVTSSGVPNGVQLFFEAITVQMRNQVETTIGLRVAPFVLPLAVTLFVFILIA
                     NWLSVLPVQYSKDGKPHELLAPPASDINFVLALALFVFVCYHAAGIWRRGILGHPAKL
                     LKGHVVFLAPINLVEELAKPISLSLRLFGNIFAGGILVALIALFPPYIMWLPNAIWKS
                     FDLFVGAIQAFIFALLTILYFSQSMELEEHHD"
     gene            complement(4054..4500)
                     /locus_tag="VF874_17750"
     CDS             complement(4054..4500)
                     /locus_tag="VF874_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX9177832.1"
                     /translation="MTTPAQDAPLVFPSVAFRPLRLLLICIALTAVAVAAAAALGHAS
                     FGMFFGVGLGLGLLNAVLVQRSVEAITAAAHPLKRKMALNSATRLLVITVIGLTIAFI
                     FRPTGLGVVFGLALFQVLLVLTTALPVMKALRKEGADTAEGSGTTS"
     gene            complement(4736..5935)
                     /locus_tag="VF874_17755"
     CDS             complement(4736..5935)
                     /locus_tag="VF874_17755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX9177833.1"
                     /translation="MVQYGFSVVSAADSVLALSERGAGVPLRELALVGLTAAIITYFC
                     TGWVRVLAMRLGAVACPRERDVHVQPTPRMGGLAMYIGVLAAVLLASQLPALMRGFVY
                     SSGMPAVLVAGGIIMGIGLIDDRWGLDALTKFAGQITAASVLVTMGVAWSVLYIPIGG
                     VGTIVLDQVSSILLTLALTVGIVNAMNFVDGLDGLAAGLGLITALAICIFSVGLLRDH
                     GGDVLFYPPAVISVVLAGACLGFLPHNFYRARIFMGDSGSMLIGLMLAAASTTAAGPV
                     SQNAYGARDVFALLSPFLLVVAVMFVPMLDLLLAIVRRTRAGRSAFSPDKMHLHHRLL
                     EIGHSHRRVVLLIYLWVGIVAFGTASTIFFDPRYAGGVMLAAIVVAIVVTVIPLLRRG
                     DELYDDE"
     gene            complement(5943..6596)
                     /locus_tag="VF874_17760"
     CDS             complement(5943..6596)
                     /locus_tag="VF874_17760"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9177834.1"
                     /translation="MTQTFDCTDEAARSTGISAAVSAVRSGRLVVLPTDTVYGVGADA
                     FDPAAVASLLAAKGRGRDMPVPVLVGSWHTIDGLAFAVPGTARELIRAFWPGALSIVV
                     QQAPSLRWDLGEGRGTVMVRMPLHAVAIELLREVGPMAVSSANISGRPPAITADDARR
                     QLGDAVEVYLDAGPSAQQAASTIVDLTGTTPRILREGPVTAREIADVLGVDATTLAD"
     gene            complement(6593..7471)
                     /gene="prmC"
                     /locus_tag="VF874_17765"
     CDS             complement(6593..7471)
                     /gene="prmC"
                     /locus_tag="VF874_17765"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX9177835.1"
                     /translation="MARLRRAIDEATRTLTDAGVASARTDAEELAAHVAGTSRGRLPI
                     LDVVGDEFFGRYAELVAERAKRIPLQHLTGTAAFGPLNLGVGPGVFIPRPETEALLEW
                     AVAQRLPAEPSALRPSGSSPPLIVDLCTGSGALAAALASHVPSARVVGVDISADALRY
                     ASRNTAGTSVELRRGDVRDPLLFDDLDGRVDLVVANPPYLPDHAELEPEVAEHDPPQA
                     LFGGPDGTSIIAAVLVLAGRWLNRGGLLAIEHDDAASATVLGMIQGAKLFEEVTARQD
                     FGGTPRFVTARRKATP"
     gene            complement(7471..8523)
                     /gene="prfA"
                     /locus_tag="VF874_17770"
     CDS             complement(7471..8523)
                     /gene="prfA"
                     /locus_tag="VF874_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX9177836.1"
                     /translation="MDALLAEHAELERRLADPELHTDQAAARKVGRRFAQLAPIVSTY
                     RKLEAARGDLEAARELAADDESFADEVTELESLVDELDTQLTDMLAPRDPHDGDDILL
                     EVKSGEGGEESALFAADLARMYIRYAERHGWTVTVLDQTTSDLGGYKEATLAIASKGD
                     AADGVWSRLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVGQVQIDESDLRI
                     DVYRSSGKGGQGVNTTDSAVRITHVPTGIVVTCQNERSQLQNKTRALQVLAARLQALA
                     EEQALADASADRASQIRTVDRSERIRTYNFPENRIADHRIGFKAHNLDQVLDGDLDAL
                     FDALALADKQSRLQQA"
     gene            complement(8647..8877)
                     /gene="rpmE"
                     /locus_tag="VF874_17775"
     CDS             complement(8647..8877)
                     /gene="rpmE"
                     /locus_tag="VF874_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX9177837.1"
                     /translation="MKSGIHPDYVETTVHCGCGNTFTTRSTKKGGQIHVEVCSQCHPF
                     YTGKQKILDSGGRVARFEKRYGKRKDKAATDS"
     gene            9350..9784
                     /locus_tag="VF874_17780"
     CDS             9350..9784
                     /locus_tag="VF874_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177838.1"
                     /translation="MKLKEVLATATVTGVLGVGALGAGGAVAGADPAPWPPGHGNDGY
                     GPSWQDNNWQSNNWQGDWNVGGRDWWRGRPGKWWRDGDLPPWGYWGAPPPVQWSGPPP
                     WEQPRTINYWGYQATPVWDDGFHGWGIWLFGIWIPIVGIGVF"
     gene            complement(9838..11550)
                     /gene="rho"
                     /locus_tag="VF874_17785"
     CDS             complement(9838..11550)
                     /gene="rho"
                     /locus_tag="VF874_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX9177839.1"
                     /translation="MSTSAPVDVPARAPQGDGTLATMVLPDLRALANEVGVKGTSGMR
                     KNELIAAIRERRGEPIGKAHNGESTTTDTDSSDGSSESRATEGTQDEQEKADADKSPS
                     DRSPSDRSPSDKGPSDKSTDEDTRPETRRDGDQPQGGDQQGGQNRGDDDGEGRQGRRG
                     RRYRDRRRRERGGGDGGADAELREDDVVQPVAGILDVLDNYAFVRTSGYLAGPNDVYV
                     SMNMVRKNGLRRGDHITGAVRVPKDGEQPNQRQKFNPLVRIDSVNGGSVEDAKKRPDF
                     GKLTPLYPNQRLRLETTPDRLTTRVIDLIMPIGKGQRALIVSPPKAGKTTILQDIANA
                     ITKNNPECHLMVVLVDERPEEVTDMQRSVKGEVIASTFDRPPSDHTSVAELAIERAKR
                     LVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPPKRFLGAARNIEEG
                     GSLTIVATAMVETGSTGDTVIFEEFKGTGNAELKLDRKIAERRVFPAVDVNPSGTRKD
                     ELLLSPDEFAVVHKLRRVLSGLDSHQAIDLLMSQLRKTKTNYEFLVQVSKNAPGPVNE
                     EFAS"
     gene            complement(11885..12835)
                     /gene="thrB"
                     /locus_tag="VF874_17790"
     CDS             complement(11885..12835)
                     /gene="thrB"
                     /locus_tag="VF874_17790"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX9177840.1"
                     /translation="MSQLLPVGLTCRAVVAASAANLGPGFDSLGLALSLYDEIVVTTI
                     DSGLQVEVEGEGAGHVPLGREHLVVRAVERGLDAAGVAAPGLAVRCRNDIPHSRGLGS
                     SAAAAVGGLAAANGVLAQGHSKPLSTPQLIQLASEFEGHPDNASAAVLGGAVVSWTES
                     DGSREAYSAVPLRLHPDIRIFSAIPEERSLTAATRVLLPSLVSHEDARFNVSRVALLV
                     VALTERPDLLLAATEDVLHQPQRATAMPTSSEYLKLLRRYGLAAALSGAGPAVIALTT
                     QPELPAEILEFGAANGFAVREMTVGEGVRWTSDVVARRAR"
     gene            complement(12832..13914)
                     /gene="thrC"
                     /locus_tag="VF874_17795"
     CDS             complement(12832..13914)
                     /gene="thrC"
                     /locus_tag="VF874_17795"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9177841.1"
                     /translation="MTATKSKVHQPWPGLIAAYRDRLPVGDDWTPVTLLEGGTPLVPA
                     PRLSELTGCTVHLKVEGANPTGSFKDRGMTMAVTDALARGQQAVLCASTGNTSASAAA
                     YAARGRITCAVLVPQGKIAMGKLAQAVMHGAKIIQVDGNFDDCLELARKTTADFPTVA
                     LVNSVNPVRIEGQKTAAFEIVDALGTAPDVHSLPVGNAGNITAYWRGYTEYHRDGLSD
                     RLPRMLGTQAAGAAPLVLGEPVKEPETIATAIRIGSPASWGPAVEAQQQSHGRFVAAT
                     DDEILAAYHLVARSEGVFVEPASAASIAGLLKSVEDGWVTKGSMVVCTVTGNGLKDPD
                     TALKGIPAVSAIPVDPMAVVEKLGLV"
     gene            complement(13911..15236)
                     /locus_tag="VF874_17800"
     CDS             complement(13911..15236)
                     /locus_tag="VF874_17800"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX9177842.1"
                     /translation="MSSAEKPVGVAVLGLGNVGSQVVRIIEDSAADLAARIGAPLELR
                     GIGVRRVAPDRGVPVELLTDDVDALVSRNDVDIVVELMGPVEPAHKAILGALERGKSV
                     VTANKALLAQSTGELAQAAERAHVDLYFEAAVAGAIPVIRPLTQSLAGDSVLRVAGIV
                     NGTTNYILSEMDSTGADYETALADASALGYAEADPTADVEGYDAAAKAAILASIAFHT
                     RVTADDVYREGITKVTPADFASARALGRTIKLLSICERITGDDGQERVSARVYPALVP
                     LSHPLANVNGAFNAVVVEAEAAGRLMFYGQGAGGTPTASAVMGDVVMAARNRVQGGRG
                     PRESKYAQLPIAPMGMIPTRYYVSMYVADRPGVLATVATEFAKHEVSIAEVRQEGVVD
                     EGGQRVGARVVVVTHEATDAALSDTVAALAGLDVVQNVASVLRLEGSGE"
     gene            complement(15233..16573)
                     /gene="lysA"
                     /locus_tag="VF874_17805"
     CDS             complement(15233..16573)
                     /gene="lysA"
                     /locus_tag="VF874_17805"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9177843.1"
                     /translation="MGGLPPNVWPHNVVRGDDGEVRVAGVRVTDLAGEFGTPLFVIDE
                     DDFRERCRVIAVAFGGGANVHYAAKAFLCTEVARWIAEEGLSLDVCSGGELAVALHAG
                     FPPERIALHGNNKSVDELTAAVRAGIGHVVLDSMTEIERLDAITGQLGTVQDVLVRVT
                     VGVEAHTHEFISTAHEDQKFGLSLADGQAMAAIRRIFETDHLRLVGLHSHIGSQIFDV
                     AGFELAAHRVIGLLRDVVTELGVQKTSQISTIDLGGGLGISYLPYDDPTPVEELAAKL
                     SVIVRNESALAGLPERRLVVEPGRAIAGPGTITLYEVGTVKDVAVTPHAHRRYVSVDG
                     GMSDNIRTALYGAEYDVRLVSRASDAPATLARIVGKHCESGDIVVRDTWVNDDIRPGD
                     LLAVAATGAYCYSMSSRYNLIGRPAVVAVRGGRARLILRRETVDDLLSLEVTSA"
     gene            complement(16654..18306)
                     /gene="argS"
                     /locus_tag="VF874_17810"
     CDS             complement(16654..18306)
                     /gene="argS"
                     /locus_tag="VF874_17810"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX9177844.1"
                     /translation="MTPADLADLLKNTAAAVLTEHGLDTAALPATVTVERPRNPEHGD
                     YATNLALQLGKKVGVNPRELAGWLAERLSGVDGIASADVAGPGFVNLRLAASAQGVIV
                     GNVIDAGRNYGRSETLRNQKINLEFVSANPTGPIHIGGTRWAAVGDALGRLLSSQRAE
                     VVREYYFNDHGAQIDRFTRSLITAAKGQPVPDDGYAGSYISEIAAQVLEKAPDALSQP
                     EAEQHETFRSIGVDLMFSHIKQSLHDFGTDFDVYTHEDSMHTSGRVEQAIARLRETGN
                     IYEKDGATWLRTTEFGDDKDRVVIKSDGQPAYVAADIAYYLDKRDRGFDLCIYMLGAD
                     HHGYIARLKAIAAALGEDPDTVEVLIGQMVNLVRDGQPVRMSKRAGTVITLDDLVEAV
                     GVDAARYSLIRSSVDTPIDIDLALWSSASSENPVYYVQYAHARLCALARNAAELGLVP
                     DTGHLDLLTHDREGTLIRNIGEFPRVLKTAAAQREPHRISRYLEDLAGDYHRFYDTCR
                     VLPQGDEAPTDLHTARLALCQVTRQVIANGLEILGVTAPERM"
     gene            18420..18492
                     /locus_tag="VF874_17815"
     tRNA            18420..18492
                     /locus_tag="VF874_17815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18453..18455,aa:Arg,seq:ccg)
BASE COUNT         3098 a         6193 c         6235 g         3260 t
ORIGIN      
        1 accctggttg aacaggtcgc tctccaggaa gcactggccg tcggtgatcg agatgacgtt
       61 ggtggggatg taggccgaga tgtcgttggc cttggtctcg atgatcggca ggccggtcag
      121 cgaaccaccg cccatctcgt cggacagctt cgcacaccgc tcgagtagcc gagagtgcag
      181 ataaaacacg tcacccgggt aggcctcacg acccgggggc cggcgcagca gcagtgagat
      241 cgcccggtag gcctcggcct gcttgctcag gtcgtcgaac acgatcagca catgcttgcc
      301 gtcgtacatc cagtgctggc cgatggccga accagtgtag ggcgcaagcc atttgaaccc
      361 ggccgagtcc gacgccggcg cggcgacaat agtagtgtag tccatcgcgc caccctcttc
      421 cagcgcgttg tgcacgctgg cgatcgtggt gcccttctgg ccgatcgcca cgtagacgca
      481 gcggacctgc tgcttctcgt cgccgctctc ccagttctgc cgctggttga ggatcgtgtc
      541 cacacagatc gaggtcttgc cggtcttgcg gtcaccgatg atcagctggc gctggccccg
      601 gccgatcggg gtctgcgcgt cgatcgcctt gatgccggtc tgcagcggct ctttcacgcc
      661 ctgccgctgc accactgacg gcgcctgaat ctccaaagcc cggcgctcgt ctgtctcgat
      721 gtcgccgcgg ccgtcgatgg gctggccgag cgggttgacc acgcggccca ggaatgcgtc
      781 gccgaccgga accgaaagaa cctcaccggt gcgcttgacc tgctggcctt ctgcgatctg
      841 ttcgaagttg cccaagatca cggcacccac gttgtgttcg tcgaggttca gcgcgacgcc
      901 cagcacgccg ccctcgaact cgagcagttc ctgggtcatc acggacggca gcccctcgac
      961 gtgggcgatg ccgtcgccgg tatcgatgac ggtgccgacc tcctcacgcg ccgtgtcggc
     1021 ggtgaagctg gagacgtact cctcgattgc gccctggatg tcgtcgggcg aaatcgtcaa
     1081 ctctgccatg gcttttcgtc ttcctgcttt gggtatggat ctctatttaa gttcagtcgg
     1141 gtagccggtt ccgggcctcc gccagccgtg acgacagcgc tccgtcgatc acctcgtcgc
     1201 cgatggagac cgtcagaccg cccagcagct tggggtcgac ctccagctgc acggataccg
     1261 gacggctgta gatgcgactc agtacctccg tcagccgatt gcgttgcgcg tcgctgagct
     1321 cggcagcggc tttcgcgtgc gctatgactt cgtcgcgacg cgcgaccgcc agttgcgcta
     1381 gcgccgcaac ggcttcgtcg gcgcggccgc cgcggagcag atcgaccgtt tgcttgagaa
     1441 gcgccgcggt ggtcggattc accccctggc tgcggttgag cacattctcg agcagccaca
     1501 cgcgcccgtc cgccggccgc gtgtggtcac tgagcagagt tcccaattgt ggttccgcgt
     1561 cgagaatgcg gccgaaccag aacaactgct cctcgacctc ttcggcgtgg tgtccgcgtt
     1621 cggcccgaac catcagcgcc agtcgggcga cgtgctcgac ggcgtccacc aggttggaat
     1681 cagaggacca tcgtccggat accgccgcct tgaggatgtc cagggccgaa tcgccgactt
     1741 gtcctccgag cacggattcc aacaggcgcg ccttcggttc ggggtcattc gacgggtcgg
     1801 ccaggtgcct ggtcaggatg gtctcacggt tgaacagctt ggtgacggaa gccaattcat
     1861 cggccagagt ttccaattgc ccgacgttga ggtcgccggt cacctcgtcg aaccgctcga
     1921 tcaacgtgtt gagcgcttcc cggctggctg aacgcagctt ggccgtcgcg gggtcctcga
     1981 tggtcatgct cgacggcgcc atctcgtcga gcccgtcgag gaaccggtcg acggtggccg
     2041 cctgcgcatc ggagtcggag acgtggtcgc ggaccaggcc accggcgcgc cgcacggctt
     2101 cgctgcccag atcttgacgc agctggcgga cgaactgagc cctgagcagc tgaatgtgct
     2161 gctggccttg cactttgacg cgttcgactt ccgcgtcggc ttggactcgc agcagttcag
     2221 cgatgctctt ggcgtccgca cgggcttcct cgatgacccg cttggcctct gcttcggcct
     2281 cctgcagcgc cttctcgtgt gcctgtttgg cctccacgag ccgtttctct gctgcggcac
     2341 tgtcctcgag ctgcttgcga accgcgtcct ggcggttcgc catcaacctg cgcactggcg
     2401 gaacgacgta gcgccacacc agaaacgcga tgaccgcgaa cccgaccagc tgcccgataa
     2461 acgtcgacat ggcgttatcg tcccgtcttt gcggcggctt cctgaccgac gtcgacgccc
     2521 agcacccggc tggccaatgt ggccgccagt gtctccatcg acgccctcag gtcggcgctg
     2581 atggcgtccg actgctgctt gagctcttct gtcgccttct gcatcgccaa cgccgcctgc
     2641 tcggaggcac gccccttcat gtcctcgagg attttgcggc cctcgctacg cgcctcatcg
     2701 cggacgccgg atgcttcacg ccgagcccgc gccatctcgg cccggtagtc ggcgtcggcc
     2761 gccgcgaact gcttgtccgc ttggcggccg ttctccatgg tcttggtgac catctcgtcg
     2821 cgctcgtgca gcaccctcat gacgggcggc acgacgaagg tgccgatcac gccgagcacg
     2881 atcaagaaga tgatcagcac gacgaagaag gtgccgttcg ggacgaggaa gttgctttgt
     2941 ccgccttcct cagcgacgaa cgtcatggtc acctcaccca tcgcggcgaa ctacttgacc
     3001 ggggtggcga acacgaacag cgccatgaac gccaggttga tgaagtaggc ggcctcgacc
     3061 agaccgaccg tgatgaagaa cggagtgaac agccggccct gcgcctcggg ctgccgcgcg
     3121 ataccggcga tcagcgcgtt accggcgata ccgtcaccga taccggcgcc gatagcgccg
     3181 ccggccatga tcaacccacc gccgatgaga gcgccggcag cgattgtggg atccattcct
     3241 atcctccttg ataactggta gtggacctac caggactggt cataccggtg gtgcaggtgt
     3301 gcggtcagtc gtgatgctcc tcgagctcca tcgattgact gaagtacaag atcgtcagca
     3361 gcgcgaagat gaacgcctgg atcgcaccga cgaacaagtc gaagctcttc cagatcgcgt
     3421 tgggcagcca catgatgtac ggcgggaaca gcgcgatcag cgcgaccagg atgccgccgg
     3481 cgaaaatgtt gccaaaaagc cggagcgaca acgaaatcgg cttagccagc tcctcgacga
     3541 ggttgatcgg cgcgaggaac acgacgtgac ctttgagcag cttcgccggg tgtccgagaa
     3601 taccgcggcg ccagatgccc gccgcgtggt agcagacgaa cacgaacagc gccaacgcca
     3661 gcacgaagtt gatgtccgat gcgggcggcg caagcaactc gtgcggtttg ccgtccttcg
     3721 agtactggac gggcagtacg gaaagccagt tggcgatcag gatgaagacg aagagcgtca
     3781 cggcgagcgg caacacaaat ggggccacgc gcaagccgat ggtggtctcg acctggttgc
     3841 gcatctgcac ggtgatggct tcgaagaaca gctgaacacc gttgggaaca cccgacgacg
     3901 tgaccttggc ccgcaggaac aacgccagcg cgatcacgat caccgcggcg atggccgtgg
     3961 ccagaatggt gtcggtgttg acggtaaggc cgaaccactt ggcggtttcg tggtggccga
     4021 cctcgatggc gccttcggcg aggagggtct cagtcatgac gtcgtgccac tcccttcagc
     4081 cgtgtcggcc ccttctttgc gcagggcctt catgaccggc aatgccgtcg tcaacaccag
     4141 cagcacctgg aacagcgcca gcccgaatac cacacccaat cccgtcggcc ggaagatgaa
     4201 cgcgatcgtc aacccgatga ccgtgatcac cagaagccgg gtggcggagt tcagcgccat
     4261 cttgcgcttc agcggatgcg ccgcggcggt gatcgcctcc accgagcgct gcaccagtac
     4321 ggcgttgagc aaacccaggc ccagcccgac gccgaagaac atcccgaacg acgcatgacc
     4381 caacgctgcg gccgcggcca ccgctacggc ggtcaacgcg atgcagatga gcagcagccg
     4441 gagtgggcga aacgcgacgg aaggaaacac caacggcgcg tcctgcgctg gcgtcgtcac
     4501 cttgctacct caatcccgcg tgtctggaag atggctgaac gattccgggg tggcccgccg
     4561 agcgtatcgg agggcaacgg gcgcggtgga atcacccaag gggcatctcc ctatgtcggt
     4621 cgttctcgtc tcgtccggag gtggctccga cgagccgggg gccggcaacc cgcggtggta
     4681 ctttcgggtt ctaccagcac cgtaccacat ggtaggaagg cctaaccgcc acctactact
     4741 cgtcgtcgta cagctcgtcg ccgcgccgca gcagagggat caccgtgacc acgatcgcga
     4801 cgacgatggc cgccagcatc accccgccgg cgtagcgcgg gtcgaagaag attgtgctcg
     4861 cagtgccgaa cgcgacgatg cccacccaga ggtagatcag cagcaccact ctccgatggg
     4921 aatggccgat ctccaatagc cgatggtgca ggtgcatctt gtcggggctg aatgcgctgc
     4981 gaccggcgcg ggtgcgccgc acgatcgcga gcagcaaatc gagcatcggg acgaacatga
     5041 cagcgacgac tagcaaaaac ggcgacagta gagcgaacac atctcgcgcc ccgtaggcgt
     5101 tctgtgacac cggtcccgct gcggtggtgg aagcggcggc cagcatcaga ccgatcagca
     5161 tcgaaccgga gtcgcccatg aagatccgtg cgcggtagaa gttgtgcggc aggaagccca
     5221 ggcatgcccc cgccagcacc accgagatca ccgccggcgg gtagaacagc acatcgccac
     5281 cgtggtcgcg cagcaaacca accgagaaga tgcagatcgc caacgccgtg atcagcccca
     5341 gacccgcagc gagaccgtcg agcccgtcga cgaagttcat cgcgttcacg atccccaccg
     5401 tcagagcgag cgtcaacaga atcgacgaca cctggtccaa gacgattgtc ccgactcccc
     5461 cgatcgggat gtagaggacg ctccaggcca cccccatcgt gacgagcacg ctcgccgcgg
     5521 tgatctgacc ggcgaacttc gtcagcgcgt caaggcccca ccggtcgtca atcaggccga
     5581 ttcccatgat gattccgccc gcgacgagaa cggcgggcat tcccgaggag tagacgaatc
     5641 cccgcatcag cgcgggcagt tgcgaggcga gcagcacggc cgccagcacc ccgatgtaca
     5701 tcgcaagacc acccatgcga ggcgtcggct gcacgtgcac gtcgcgttcc cgcggacagg
     5761 ccaccgcgcc gagccgcatc gccagcacgc ggacccagcc cgtgcagaag taggtgatga
     5821 tcgccgcggt caatccgacg agagcaagct cgcgcagtgg aacaccagcg cctcgctcag
     5881 aaagggcgag cacgctgtcg gctgccgaga ccaccgaaaa accgtactgc accaagctga
     5941 gatcaatccg ccaacgtggt ggcatcaacg ccgagcacat cggcgatttc gcgggccgtg
     6001 acgggcccct cgcgcaggat gcgcggggtc gtgccggtga ggtcgacgat cgtcgacgcc
     6061 gcctgctgcg ccgacggccc ggcgtcgagg tagacctcca cggcatcacc caactgccga
     6121 cgggcgtcgt cggcagtgat tgcgggtggc cggcccgaga tgttcgcgct cgacacggcc
     6181 atcgggccga cctcgcgcag caactcgatg gcgacggcgt gtagcggcat gcggaccatt
     6241 acggtgccgc gcccctcgcc cagatcccac cgcagcgacg gcgcttgctg gacgacgatg
     6301 ctcagcgcac ccggccagaa cgcacggatg agttcccgtg ctgtgccggg aaccgcaaac
     6361 gccaggccgt cgatcgtgtg ccacgatccg accagcaccg gcaccggcat atcgcggcct
     6421 cgtcccttcg ccgcaagaag cgatgcgacc gcggcagggt cgaacgcgtc ggcgcccacg
     6481 ccgtacacgg tgtcggtggg gagcacgacg aggcggccac ttctcacggc gctgaccgcg
     6541 gcggaaatac ccgtcgaacg cgcagcctcg tcggtgcagt cgaaggtctg ggtcacggtg
     6601 tggccttcct tcgtgcagtg acgaagcgcg gggtcccgcc gaagtcctgg cgcgccgtaa
     6661 cctcctcgaa cagcttggcg ccctggatca ttccgaggac ggtggccgac gcggcatcgt
     6721 cgtgctcgat cgcaagcagc ccaccgcggt tcaaccatcg cccggcgagc accagaaccg
     6781 cggcgatgat cgatgtgccg tccggtccgc cgaacagcgc ttgtggcgga tcatgttctg
     6841 cgacttcggg ttccagctcg gcgtggtctg gcaggtaagg cggattcgcg acgacgaggt
     6901 cgacgcgccc gtcgagatcg tcgaagagta gcggatcgcg aacgtcgccg cggcgcaatt
     6961 ccaccgatgt gccggcggtg ttgcggctgg cgtacctcaa cgcgtcggcc gagatgtcga
     7021 cgccgaccac tcgggccgac ggtacgtgag aggccagcgc cgcggcgagc gcacccgatc
     7081 cggtgcacag atcgacgatc aacggcggcg atgagccgct cgggcgaaga gcagaaggct
     7141 ccgccggaag gcgttgcgcc acagcccatt cgagaagggc ttccgtctcg ggccgcggga
     7201 tgaacacgcc cggtccgacg ccgaggttga gcggtccgaa ggcggcggtt ccggtgaggt
     7261 gctgcaacgg aattcgcttc gcccgttcag cgacgagttc ggcgtagcga ccgaagaatt
     7321 cgtcgccgac gacgtcaaga atcggcaacc gaccgcggct ggtgcccgcg acgtgggcgg
     7381 ccagttcctc cgcgtcggtg cgggcggagg caacccctgc gtcggtcagc gtgcgagtgg
     7441 cctcgtcgat cgcccgccgc agccgcgcca tcatgcttgc tgcagacgag actgcttgtc
     7501 ggcgagcgca agtgcgtcga acagcgcgtc gaggtcgccg tcgagcacct ggtcgaggtt
     7561 gtgggccttg aacccgatgc ggtgatcggc gatccggttc tccgggaagt tgtaggtgcg
     7621 gatgcgttcg cttcggtcga cagtgcgaat ctggctggct cggtccgccg atgcgtcggc
     7681 gagggcctgc tcttcagcca gcgcctgcag tcgggccgcg agcacctgca aggcacgggt
     7741 cttgttctgc agctgcgagc gctcgttctg gcaggtgacg acgatcccgg tgggaacatg
     7801 ggtgattcgc acggccgagt cggtggtgtt gacgccctgc ccgcccttgc ccgacgaccg
     7861 gtacacgtcg atgcgcaggt cggactcgtc gatctgcacc tggccgactt cctccggttc
     7921 ggggtagacc aacacgccag ccgcggaggt gtgcacgcgg ccctgcgact cggtgacggg
     7981 tacccgctgc acgcggtgga cgccgccttc gaacttcagc cgcgaccaca ccccgtcggc
     8041 cgcgtcgccc ttgctcgcga tcgccaatgt ggcttccttg tatccaccga gatcggacgt
     8101 cgtctggtcg agcaccgtga ccgtccaccc gtgccgctcg gcgtatcgga tgtacatccg
     8161 tgccagatcc gcggcgaaca atgccgactc ttcgccccct tcaccggatt tcacctcaag
     8221 caggatgtcg tcgccgtcgt gcgggtcgcg cggtgcgagc atgtccgtca actgggtgtc
     8281 gagttcgtcg accagcgatt cgagttcggt gacctcgtcg gcgaacgact cgtcgtcggc
     8341 cgccagctca cgtgcggctt ccagatcgcc acgggcggcc tcgagcttcc ggtacgtcga
     8401 cacgatgggc gccagctgcg caaagcgacg cccgaccttg cgggcagcgg cctggtcggt
     8461 gtgcaattcc gggtcggcca ggcggcgctc gagttcggcg tgctcagcca gcagcgcgtc
     8521 gatcgccggc gcgccggatt ccgcgctgtt gctctgcgcc accttcctca cctccctcga
     8581 actgtgctcg aacgcaaacc gacgcccgtc cgcgcttgcg cagcagatcg ggcgtcggat
     8641 aagcagctag ctgtcggttg cggccttgtc cttgcgcttg ccgtagcgct tctcgaagcg
     8701 cgcgacacgg ccgccactgt cgaggatctt ctgcttgccg gtgtagaagg gatggcactg
     8761 cgagcagacc tcgacgtgga tctgaccgcc cttcttggtg ctgcgcgtgg tgaacgtgtt
     8821 gccacagccg cagtgcaccg tcgtctcgac gtagtcaggg tgaatacccg atttcatgga
     8881 tgtcctcttc aatcgtggcc ccgggtcgcc tggcctgcgg catcagacgt gaaccgggac
     8941 cgaggtgtcg gcgattgatt atgccaggtc agccgccacc aatgcgaaac gcccgtggcc
     9001 ggtgaactat tccgcgcggc acgatcgcgt gctctcgccg cggatgggtc gtcaacctcc
     9061 cgacggcgag ccgtcacgac caaggcgatc gcacagcatc agtccagttc agagaccact
     9121 ttgcggctgt ccatacctgc agtcgttctg ctgtaacggc ccgatcagcc gacccgatag
     9181 acgcgttagg gggtcgtggg tccgtcttac gggcaacggt gcggtgaggg acccgtttct
     9241 tcggcatagc ggccgattac cggtgcggct gccgaagttg acaacaaggt gacagcttcg
     9301 gacctgtcgg ggcgattcga agttcagggt cgcaatctag gagtgataca tgaagctgaa
     9361 agaggttctg gccacagcaa cggtcacggg tgtcttgggc gtcggggcgc tcggcgcggg
     9421 gggcgctgtg gcgggtgccg atccggcgcc gtggccgcca ggtcacggta acgacggcta
     9481 cggcccgtca tggcaggaca acaactggca gtccaacaac tggcagggcg attggaacgt
     9541 cggtggcaga gactggtggc gaggacgtcc cggcaagtgg tggcgcgacg gcgacctgcc
     9601 gccgtggggc tattggggag cgcctccgcc ggtccagtgg tcggggccgc cgccgtggga
     9661 gcagccgcgc acgatcaact actggggtta ccaggccaca ccggtatggg atgacggctt
     9721 ccacggctgg gggatctggt tgttcggcat ctggattccg atcgtcggca tcggcgtctt
     9781 ctaacaagcc gaaacaacaa cggccgcctt gcttttcggc agggcggccg ttggccttta
     9841 ggaggcgaac tcctcgttca ccgggccggg cgcattcttg gacacctgaa ccaggaactc
     9901 gtaattggtc ttcgtcttgc gcaactgcga catcaacagg tcgatggcct ggtgcgagtc
     9961 gagacccgac agcacacgac gcagtttgtg cacgaccgcg aattcgtccg gggacagcaa
    10021 caattcgtcc ttacgggtgc ccgacgggtt cacgtcgacg gccgggaaaa cgcgccgctc
    10081 ggcgatcttg cggtcgagct tcagctcggc gttgccggtg cccttgaact cctcgaagat
    10141 cacggtgtcg cctgtggacc cggtctcgac catcgcggtc gcgacgatcg tcagcgaacc
    10201 gccctcctcg atgttgcgag ccgcgccgag gaaccgctta ggcggataca gggcggtcga
    10261 gtcgacacca ccggagagaa tccggcccga ggccggtgac gcgttgttgt acgcgcggcc
    10321 cagtcgggtg atggagtcga gcagcacgac gacgtctttg ccctgctcga cgaggcgctt
    10381 ggcccgctcg atggcgagct cggcgaccga cgtgtggtcg gacggcggac ggtcgaaggt
    10441 cgaggcgatg acctcgccct tgaccgaacg ctgcatgtcc gtcacctctt cgggccgctc
    10501 gtcgaccagc acgaccatca gatggcattc ggggttgttc ttggtgatcg cgttcgcgat
    10561 gtcctgcagg atcgtggtct tgccggcctt cggcggtgac acgatcagcg cgcgctggcc
    10621 cttgccgatc ggcatgatca ggtcgatgac acgggtggtc agccgatcgg gcgtggtctc
    10681 caggcgcagc cgctggttcg ggtacagcgg ggtgagcttg ccgaagtcgg gacgtttctt
    10741 ggcgtcctcg acggacccgc cgttgacaga gtcgatacgc accagcgggt tgaacttctg
    10801 ccgctgattg ggctgctcgc cgtccttggg cacgcgcacg gcgccggtga tgtggtcacc
    10861 acggcgcagc ccgttcttgc ggaccatgtt catcgagacg tacacgtcgt tcgggcccgc
    10921 caggtagccg gaggtgcgga cgaacgcgta gttgtcgaga acgtccagga tgccggccac
    10981 gggctgcacc acgtcgtctt cgcgcagctc ggcgtcggcg ccgccgtcac cgccgccgcg
    11041 ttcccggcgc ctccgatcgc ggtaccggcg accccgccgg ccctgccggc cttcaccgtc
    11101 gtcgtcgccg cggttctggc cgccctgctg gtccccgcct tgcggttggt ctccgtctcg
    11161 tcgggtctcc ggcctggtgt cctcgtcggt cgacttgtcg gaagggccct tgtcggaagg
    11221 gctcctgtca gacgggctcc tgtcggacgg gctcttgtcg gcgtccgcct tttcctgttc
    11281 gtcctgggtg ccctcggtgg cgcgactctc gctgctcccg tcactgctgt ccgtgtcggt
    11341 cgtcgtgctt tcgccgttgt gggccttgcc gattggctct ccgcgacgtt cgcggatcgc
    11401 ggcgatcaac tcgttcttcc gcatgcccga ggtgcccttg acgccgacct cgttggccag
    11461 cgcgcgcagg tccggcagca ccatggtcgc gagcgtgccg tcgccttgag gggcgcgggc
    11521 cggcacgtcc acgggcgccg aggttgagat gtcaggtgtc gcggtggtgg gcagatccgg
    11581 ttcttcggcg ccactgccgg ccgctatgag gtccgtatcg gtcacggatg tcctttctct
    11641 ccctcgcagt tttcgtcata cgaggggttc ggtgcactca gccgatccgc tgaatgctta
    11701 tgtccccacc gtcgtctgtg caacggtgat cgcactggtt cgcagctgta aggcgaaccg
    11761 tcatccccga gaagaatttg gtgatcgtcc tactgtcggc gcaggcagaa gctgcgattg
    11821 ccggacggca ccgaggataa cccgcttccg gacgggaagc aagaaacctc ggaaacccca
    11881 cccattaccg agcgcgtcgc gccaccacat cagaggtcca cctgacgccc tccccgaccg
    11941 tcatctcgcg gacggcgaag ccgttggccg caccgaactc gaggatttcg gccggcagtt
    12001 ctggctgggt agtcaacgcg ataaccgctg gtccagcccc ggaaagcgcc gccgcaagac
    12061 cataacgccg cagcagcttt aggtattccg acgacgtcgg catcgccgtg gcgcgctgcg
    12121 gctgatgaag cacgtcctcg gtggcggcga gcagcaggtc gggccgctca gtcagcgcga
    12181 caacgagcag cgcgacgcgg ctgacgttga aacgcgcgtc ctcgtggctg acaagactcg
    12241 gcagcagcac tcgggtggct gccgtcagcg accgttcctc ggggattgcc gagaagattc
    12301 ggatgtcggg gtgcagccgc aacggcacag ccgaatacgc ttcgcgggag ccgtcgctct
    12361 ccgtccacga caccaccgcg ccaccgagta cggcggccga cgcgttgtcg gggtggccct
    12421 cgaactcgga ggcgagctgg atcagctgcg gcgtggacaa cggcttcgag tgtccttgcg
    12481 ccagcacacc gttggcggct gcgagacccc cgaccgcagc cgcggccgac gacccgagcc
    12541 cccgcgaatg cgggatgtcg ttgcggcacc gcaccgcaag cccgggggcc gcgacaccag
    12601 cggcgtcgag tccgcgctcg actgcgcgta ccaccagatg ctccctgccg agcggaacat
    12661 gcccggcccc ctcaccctcg acctcgacct gcagaccgga atcgattgtg gtgacgacga
    12721 tttcgtcgta caggctcagc gcgaggccca agctgtcgaa gccgggaccg agattcgccg
    12781 cggacgccgc cacgacggcg cgacaggtca ggccgacggg cagaagctgg ctcacaccaa
    12841 gcccagcttt tcgacgaccg ccatcggatc gacggggatg gccgacaccg ccggtatgcc
    12901 cttcagcgcg gtgtcgggat ccttcagtcc gttaccggtg accgtgcaca ccaccatgga
    12961 gcctttggtg acccagccgt cctccaccga cttcagcagg ccggcgatgc tggccgcaga
    13021 cgccggttcg acaaacaccc cctcgctgcg cgccaccagg tgataggcgg caaggatctc
    13081 atcgtcggtg gccgccacga accgcccgtg ggactgctgc tgagcctcga ccgcgggccc
    13141 ccacgacgcc ggggaaccga tgcggatcgc ggtggcgatg gtctccggct ccttcacggg
    13201 ttcgcccagc accagcgggg cggcaccggc cgcctgcgtg cccagcatcc gcggcagccg
    13261 gtcactcagg ccgtcacggt gatactcggt gtagccacgc cagtacgcgg tgatgttgcc
    13321 ggcgttgccg accggcagcg aatgcacgtc cggcgcggtg ccgagcgcgt cgacgatctc
    13381 gaaagcggcc gtcttctgcc cttcgatgcg caccgggttg acggagttca ccagcgcgac
    13441 ggttggaaag tcggcggtgg tcttgcgggc gagttcgagg cagtcgtcga aatttccgtc
    13501 gacttggatg atcttggctc cgtgcatcac ggcctgggcg agcttgccca tcgcgatctt
    13561 gccctgcgga accagcacag cacaggtgat tctgccgcgc gcggcgtatg ccgccgccga
    13621 cgccgaggtg ttgccggtcg atgcgcacag caccgcctgt tggccgcgcg ccagcgcgtc
    13681 ggtcaccgcc atcgtcatgc cgcggtcctt aaaggagccg gtcgggttgg cgccttcgac
    13741 cttgagatgc actgtgcacc cggtcaattc agagagcctc ggcgcaggca ccagcggcgt
    13801 gccgccctcc agcagcgtga cgggcgtcca gtcgtcaccg acgggcagcc gatcgcgata
    13861 ggccgcaatc aaccccggcc atggttggtg cactttggat ttcgtcgcag tcattcgccg
    13921 cttccttcca gtcgcagcac gctggcgacg ttctgtacga cgtctaatcc ggccagtgcg
    13981 gcgacggtgt cggacagcgc ggcgtcggtg gcctcgtggg tgaccacgac gacccgggca
    14041 ccgacccgtt gcccgccctc gtcgaccacg ccttcctggc ggacctcggc gatgctcacc
    14101 tcatgtttcg caaattcggt tgccacagtg gctaatacgc cggggcggtc ggcgacgtac
    14161 atgctcacgt agtaccgggt cggaatcatt cccatcggcg ctatcggcag ctgggcgtac
    14221 ttcgactcgc gcggtccacg gccaccctgc acccggttgc gggcggccat caccacgtcg
    14281 cccatcaccg cggacgcggt gggtgtgccg ccggcaccct gcccgtagaa catcaaccgg
    14341 ccggccgctt cagcctccac caccacagcg ttgaacgcgc cgttgacgtt ggccagcgga
    14401 tggctcaacg gcaccagcgc cggatacacg cgagccgaaa cccgttcctg accatcgtct
    14461 cccgtgatcc gctcgcagat cgacagcagc ttgatggtgc ggccaagagc acgcgcggag
    14521 gcgaagtcag cgggggtgac cttggtgatg ccctcgcggt agacgtcgtc agcggtgact
    14581 cgggtatgga atgcgatgga ggccaaaatc gcggccttgg ccgcggcgtc gtagccctcg
    14641 acgtcggcgg ttgggtcagc ctcggcgtaa ccgagcgcgc tggcgtcggc gagtgcggtc
    14701 tcgtaatcgg ctccggtgga gtccatttcg gacaggatgt agttggtggt gccgttgacg
    14761 atgcccgcca cgcgcagaac cgagtcaccg gccagcgatt gtgtgagcgg ccggatgacc
    14821 ggaatagccc ccgcgacagc ggcttcgaag tacaggtcga cgtgcgcgcg ctcagcggct
    14881 tgagccagtt cgccggtgga ttgggcgagc agcgccttgt tggcggtcac gacggacttg
    14941 ccgcgttcca gcgcaccgag aatcgccttg tgcgcgggct cgacgggccc catcaactcg
    15001 acgacgatgt cgacgtcgtt ccgggacacc agcgcgtcca cgtcgtcggt cagcaattca
    15061 acgggcacgc cgcggtccgg ggccacccgc cgcacgccga tgccgcgcaa ctccagcggc
    15121 gcgccgatgc gcgcggccag gtcggccgca ctgtcctcga tgatgcgcac cacctggctg
    15181 cccacgttgc ccaatcccaa caccgctacg cccacgggct tttcggcact actcatgcgc
    15241 tcgtcacctc caaactcagt agatcgtcga ccgtctcccg gcgcaggatc aggcgggccc
    15301 gcccgcctcg cacggccacc acggcgggac ggccgatcag gttgtagcgg ctcgacatgg
    15361 aatagcaata ggcgcccgtc gccgcgaccg cgagcaggtc cccgggccgg atgtcgtcgt
    15421 tgacccaagt atcgcggacc acgatgtcac cgctctcgca gtgctttccg acgatgcgcg
    15481 ccaatgttgc aggggcatcg ctggcgcgcg acaccaaccg cacgtcgtat tcggcgccgt
    15541 agagcgcggt gcggatgttg tcgctcattc cgccgtcgac gctgacgtag cgccggtggg
    15601 cgtggggcgt gaccgccacg tccttgaccg tgcctacctc gtagagcgtg atggtgccgg
    15661 gccctgcgat cgcgcggccg ggctccacga ccagccgccg ctcgggcaga ccggcaaggg
    15721 cggactcgtt gcggacgatg acggacagct tggccgcgag ttcctccacc ggcgtcggat
    15781 cgtcgtaggg aaggtacgaa atgcccagtc cgccaccgag gtcgatcgtc gaaatctgcg
    15841 acgtcttctg cacgccgagt tcggtgacga cgtcgcgcag cagcccgatc acgcggtgcg
    15901 ccgcgagttc gaacccggcg acgtcgaaga tctgtgagcc gatgtggctg tgcaggccga
    15961 ccagtcgcag gtgatcggtt tcgaaaatgc ggcgaatggc cgccatcgcc tggccgtccg
    16021 ccaacgacag gccgaacttc tggtcttcgt gtgcggtgga gatgaattcg tgggtgtggg
    16081 cctccacgcc gaccgtgacg cggacgagca cgtcctgcac ggtgcccagt tggccggtga
    16141 tggcgtcgag ccgttcgatc tcggtcatcg aatcgagcac cacgtgcccg atgccggcgc
    16201 gcacggcagc ggtcagctcg tcgacggact tgttgttgcc gtgcaaggca attcgctcgg
    16261 gcgggaagcc ggcgtgcagc gcgaccgcga gctcaccgcc ggagcagaca tccagcgaca
    16321 gcccttcctc ggcgatccag cgcgcaacct cggtgcacag gaacgccttg gcggcgtagt
    16381 ggacattcgc cccgccgccg aacgcgaccg cgatcacgcg gcatcgttcg cggaagtcgt
    16441 cctcgtctat cacgaacagc ggggtgccga actcgccggc gaggtccgtc acgcgcacac
    16501 cagcaacgcg cacctcaccg tcgtcaccgc ggacgacgtt gtggggccag acatttggcg
    16561 ggagccctcc cagctcttgc ggtgtctgcg gcttctcggg tgctgcggcg gggtggactt
    16621 cctcggcatg gcgcggtccg gcgggatgag cgatcacatg cgctccgggg cagtgacacc
    16681 gagaatttcc aggccgttgg cgatgacctg gcgagtaacc tggcacagcg caagtcgcgc
    16741 ggtgtgcaag tcggtgggtg cctcgtcgcc ctgcggcagc acacggcagg tgtcgtagaa
    16801 ccggtggtag tcgccggcca ggtcttccag ataacggctg atgcgatggg gttcacgctg
    16861 cgccgcagcg gttttcagca cgcgcgggaa ctcaccgatg ttgcggatca gcgtgccttc
    16921 gcggtcatgg gtgagcagat cgagatggcc ggtgtcgggg acgagtccga gctcggcggc
    16981 gtttcgggcg agcgcgcaga gccgggcatg cgcgtattgc acgtagtaga ccggattttc
    17041 gcttgacgcc gacgaccaca gcgcaaggtc gatgtcgatc ggcgtgtcta ccgacgaccg
    17101 gatcagcgag tagcgggcgg cgtcgacacc gacggcctcg acgaggtcgt cgagggtgat
    17161 aacagtgccg gcccgcttgc tcatccggac aggctggccg tcgcggacca ggttcaccat
    17221 ctggccgatc agcacttcga cggtgtccgg gtcctcgccg agcgcggccg cgatggcctt
    17281 gagccgagcg atgtaaccgt ggtggtctgc cccgagcatg tagatgcaca ggtcgaagcc
    17341 acggtcccgc ttgtcgaggt agtaggcgat gtcggcggcg acgtaggcgg gctgaccgtc
    17401 gctcttgatg acgacgcggt ccttgtcgtc accgaactcg gtggtgcgca accacgttgc
    17461 gccgtccttc tcgtagatgt tgccggtctc gcgcagccgg gcgatggcct gctcgacgcg
    17521 gccgctggtg tgcatcgagt cttcgtgggt gtagacgtcg aagtcggtgc cgaagtcgtg
    17581 cagggattgc ttgatgtggc tgaacatcag gtcgacgccg atggaccgga aggtctcgtg
    17641 ctgctctgcc tcgggctggc tcagcgcgtc gggtgccttt tcgagcactt gagcggcgat
    17701 ctcggagata tagctgccgg cgtagccgtc gtcgggaacg ggctggccct tggcggcagt
    17761 gatcagcgag cgggtgaacc ggtcgatctg ggcgccgtgg tcgttgaagt agtactcgcg
    17821 taccacctcg gccctctggc tggagagcag ccggccgaga gcgtcgccga cggcggccca
    17881 acgggtgccg ccgatgtgga tcgggccggt gggattcgcc gagacgaact caaggttgat
    17941 cttctgattc ctcagcgtct cggagcgccc atagtttcgg ccggcgtcga tgacgttgcc
    18001 gacgatgacg ccctgggccg acgccgcgag gcgcaggttg acgaaaccgg gtccggcgac
    18061 gtcggcggag gcgatcccgt cgacgccgga aagcctttct gcaagccagc cggcgagttc
    18121 gcgtgggttg acaccgacct tcttgccgag ctggagtgcc agattggtgg cgtagtcgcc
    18181 gtgctcggga ttgcgcgggc gctcgaccgt gaccgtcgcg ggaagcgcgg cggtgtcgag
    18241 gccgtgctcg gtgagcaccg cagctgcggt gttcttgagc aggtcggcca ggtcggcggg
    18301 ggtcacgagg ttccatccta tgggctgggc ggatgccgtc cgaattcatt ccggcgggct
    18361 ggcaattgcc gcgatgcgct actctgggcg ggcccaacgg cgcagtgtct tggtgatgcg
    18421 cccccgtagc tcaggggata gagcgtctgc ctccggagca gaaggccgca ggttcgaatc
    18481 ctgccggggg cactcaaggt ttatgcaatt cagaccctat tttcttcccg ccggactggg
    18541 cgtgtgccat ccgcgtgcta tccggagtgg gatgctccat cgggtctcgg ctccacgcaa
    18601 ccccacctga gcggtagaca cagcatgacc accgcatccc tcaaccctct acgagactgc
    18661 gatcgtgacg ctttccccgg gtgaccttgc ggtgtggagc agccacctca tgatggtcgt
    18721 gcggtagttg tcgacgtgga ccggattaag cgaaaaaggg ccgctgaatt gtggccctga
    18781 tcgtgg
//
LOCUS       DASWBF010000483         5339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_831103,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000483 DASWBF010000000
VERSION     DASWBF010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831103"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VF874_17820"
     CDS             <1..381
                     /locus_tag="VF874_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEX9177845.1"
                     /translation="IRGRMGAVRFKAFDADDEACVASLRNTTGERDFDEAWAEGAALS
                     TEEAIAYAQRGRGERKRPASGWASLTPTERDVVRLVGEGLANKDVAARLFVSPRTVES
                     HLSHVYAKLGLSSRVQLVQEAARH"
     gene            complement(378..1673)
                     /locus_tag="VF874_17825"
     CDS             complement(378..1673)
                     /locus_tag="VF874_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEX9177846.1"
                     /translation="MTDSETTPHPRPVPRPAHPAARPSRPPGSDPHRFGRVDPDGTVW
                     LITSAGERVIGSWQAGDPDAAYAHFGRRYEDLRTEVDLLERRLASGTGDARKIRSSAA
                     ALEEGLPTAAVLGDVDAIAARLSTIRERADEAAQAEKERRDEQRAEQVARKEALAAEA
                     EELATNAVQWKTAGDRLRAILDEWRTLTGVDRKTDDALWKRYSAARETFNRRRGSHFA
                     ELDRERASAREAKEGLCERAEELADSTEWSATAATFRQLLTEWKAAGRAAKEVDDALW
                     RRFKAAQDKFFAARNAVTAERDAELHANTAAKEELLARAEKLDTTDLDAARSELHAIA
                     AKWDTIGKVPRDRGTELERRLRVVEKRVRDAGSSDWSDPQAQARAEQFSSRAEQFEQQ
                     AEKAEAAGRSAEAEEARANARQWREWAETAEGAVGRKSR"
     gene            1850..2830
                     /locus_tag="VF874_17830"
     CDS             1850..2830
                     /locus_tag="VF874_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9177847.1"
                     /translation="MAKADIAALLALGAAFFIAIGDVIHQRSAHEVADESVGHVQLFL
                     RLLRDRQWWLGSVVAAVGFSLQAAALGLGSVLLVQALLVTSLLFALPMSAKAAHRRVT
                     RWEWIWAVALAGSVAVIVTVGNPQAGQSRASLETWTAVVAVMGPVLVLCVLGARMWAG
                     SVSAVLLAFVSGALWGVFAVLTKGVVDRLDDGLWPLLRTPELHAWALLAIAGTAFQQS
                     SFRAGTMTASLPTMTVAEPVVASVLGIVVLGETLNTDDAGWLVLGLAIVAMVVATIAL
                     ARGEALASDEPVDAKTAAAPSSAPGSSIQPIHNPHAEIAEESAREDRRLP"
     gene            2835..3518
                     /locus_tag="VF874_17835"
     CDS             2835..3518
                     /locus_tag="VF874_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177848.1"
                     /translation="MLTAIAIIPSAPVLVPQLVGTAADELADLRQAVFTAAGALPARW
                     LAVGVGPADEVVGPQATGTFAGYGVDVRAGLSADAADVGELPLSALITAWVRAEARPA
                     ARVEVRVFDRDLSADEAIRRGRELRAVVDEADEPVGVLVVADGANTLTERAPGGHDPA
                     SVTVQIALDDALAAGDVAGLAELPASITGRVAWQVLAGLAGAGPRSAEELYRGAPYGV
                     GYFAGVWIP"
     gene            3515..4423
                     /gene="miaA"
                     /locus_tag="VF874_17840"
     CDS             3515..4423
                     /gene="miaA"
                     /locus_tag="VF874_17840"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX9177849.1"
                     /translation="MTRPIAIVGPTGTGKSQLALGVAEAVGGEIVNADAMQLYRGMDI
                     GTAKLPVDERRGIPHHQLDLLDVTENATVANYQRGAAADVDAIMARGAVPVIVGGSML
                     YVQSLLDDWAFPATDAAVRARWEQRLAHVGVAQLHRELAAVDAAAAESILPTDGRRIV
                     RALEVVELTGRPFAASAPTIGSPRWDTVIIGLDWETNQLDERLAKRTEQMFDAGLVDE
                     VVTLVDKGLRDGVTASRALGYAQVIADLDSGGDGAAAREPTFVATRRYVRRQRSWFRR
                     DHRIHWLDGSDDSNVENTLQAWRHVS"
     gene            4428..5294
                     /gene="dapF"
                     /locus_tag="VF874_17845"
     CDS             4428..5294
                     /gene="dapF"
                     /locus_tag="VF874_17845"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX9177850.1"
                     /translation="MIFAKGHGTQNDFVVLPDIEATLSLTPDAVAALCDRRRGLGADG
                     VLRVTTADAAVARGVLERLPEGVSGRDWFMDYRNADGSIAEMCGNGVRVFAHFLRVSE
                     LELRDEFVVGSLAGPRPVQVHGVDGTFADVTVEMGKTNQLGTGEAVVGGRTFRGLAVD
                     VGNPHLACVDPDLSEDALRALDVAAPVSFDRTQFPDGVNVEVVTAPAEGAVSMRVHER
                     GVGETRSCGTGTVAAVVAALAHDGATTGSLTVRIPGGEVDVTVTAATSYLRGPSVLVA
                     RGEISEEWWRAQ"
BASE COUNT          702 a         1868 c         1918 g          851 t
ORIGIN      
        1 attcgcgggc gcatgggcgc ggtccgcttc aaggctttcg acgccgacga cgaggcatgc
       61 gttgcgtcgt tgcgtaacac gacgggcgag cgtgacttcg acgaggcgtg ggccgagggc
      121 gcggcgctgt ccactgagga agctatcgcc tacgcacagc gcggccgcgg tgaacgcaag
      181 cgaccggcca gcgggtgggc atcgctcacc ccgacagagc gcgacgtggt acgcctcgtc
      241 ggcgagggcc ttgccaacaa ggacgtcgcc gcaaggcttt tcgtctcacc acgaacggtc
      301 gagtcccacc tgagccacgt ctacgccaaa ctcgggctgt cctcacgcgt gcagttggtg
      361 caagaagcag cccgccacta acggctcttt cttccgaccg cgccctccgc ggtctctgcc
      421 cactcgcgcc actgccgcgc attagcgcgt gcttcctcgg cttcggcgga ccggccagcc
      481 gcctcggcct tttcggcctg ctgttcgaat tgctctgcgc gggaactgaa ttgctcggcg
      541 cgggcctgtg cctgcgggtc gctccagtct gagctgccgg cgtcgcgcac ccgcttctcc
      601 accacccgca gtcgacgctc cagctccgtc ccacggtcgc gcggcacctt gccgatcgtg
      661 tcccacttcg cggcgatcgc atgcagctcg gaccgggcgg catccaggtc ggtggtgtcc
      721 agcttctcgg cacgcgccag cagctcctct ttcgcggcgg tgttcgcatg cagctcggcg
      781 tcccgctcgg cggtcacggc gttgcgcgcg gcgaagaact tgtcctgcgc cgccttgaac
      841 cgccgccaca gcgcgtcgtc gacctctttg gccgccctgc ccgctgcctt ccattcggtg
      901 agcaactgcc gaaacgtcgc ggccgtcgca ctccattcgg tggagtcggc cagctcctcg
      961 gcgcgctcgc acaggccctc cttggcttcg cgcgcgctgg cccgctcacg atccagctcg
     1021 gcgaagtgcg aaccgcgtcg gcggttgaac gtctcccgcg ccgccgaata ccgcttccac
     1081 agcgcgtcgt cggtcttgcg gtcgacgccg gtgagcgtgc gccactcgtc gaggatcgcc
     1141 cgcagccggt cgcccgccgt tttccactgc actgcattcg tcgccagctc ttcggcttcc
     1201 gcggccaacg cttccttgcg ggcgacctgc tcggcgcgtt gctcgtcgcg gcgctccttc
     1261 tcggcctgtg cggcctcgtc ggcccgctcc cggatcgtgc tcagccgggc cgcgatcgcg
     1321 tcgacgtcac ccaggacggc tgcggtgggc agaccctctt cgagggccgc cgcggaggac
     1381 cggatcttgc gggcgtcgcc ggtgcccgac gccagccgcc tctccagcag gtcgacctcg
     1441 gtgcgcaggt cctcgtagcg gcggccgaaa tgtgcgtatg ccgcgtccgg gtcgccggcc
     1501 tgccacgacc cgatgacccg ctcaccagcg gaggtgatca gccagacggt gccgtcgggg
     1561 tccacccgcc cgaaccgatg cggatcgctg cccgggggac ggcttggccg agccgccgga
     1621 tgtgccggcc gcggcaccgg cctcgggtgg ggtgtggtct cgctgtccgt catctcgtgc
     1681 acctccgcgg gctggaaccc gcacctgccg cgcttcgcgg cgcctgataa ctatggtcga
     1741 aacgacggag cccgaaagga catgaaatgt ccgtcgactc gacctccgct attcaagcag
     1801 gtcgtcgtgg tacgtgcgcc cacgttcagc caggtcccag gaggcgttga tggccaaggc
     1861 agacatcgcc gccctactcg cgctcggcgc ggccttcttc atcgcgatcg gcgacgtcat
     1921 ccaccagcgc tcggcgcacg aggtcgccga cgagtcggtc ggccacgtgc agctgtttct
     1981 gcggctgctg cgcgaccgcc agtggtggct gggcagcgtg gtcgccgcgg tcggattctc
     2041 gctgcaggcg gcggcgctgg gcctcggttc ggtgctgctg gtgcaggcgc tgctggtcac
     2101 gtcgctgctg ttcgcattgc cgatgagcgc caaagccgcg caccgccggg tgacccgctg
     2161 ggagtggatc tgggcggtgg cgctcgcggg cagcgtcgcg gtgatcgtca ccgtcggcaa
     2221 ccctcaggcc ggccagtcgc gagcgtcgct ggaaacctgg accgccgtgg tcgcggtgat
     2281 gggcccggtg ctggtgctgt gcgtactggg ggcgcggatg tgggccgggt cggtcagcgc
     2341 ggtgctgctc gcgttcgtct ccggagcgct gtggggggtg ttcgcggtgc tgaccaaggg
     2401 cgtcgtcgac cgactcgacg acggactttg gccgctgctg cgcacccccg agttgcacgc
     2461 gtgggcgctt ctcgccatcg cgggcaccgc gtttcagcag tcgtcgttcc gggccggcac
     2521 gatgaccgcg tcgctgccga cgatgacggt ggccgaaccc gtggtggctt cggtgctggg
     2581 catcgtcgtg ctcggcgaga cgctgaacac cgacgacgcg ggctggttgg tcctcgggtt
     2641 ggcgatcgtc gcgatggtgg tggcgacgat cgcgctggcc cggggagagg cgctggcctc
     2701 cgacgagccg gtggacgcga aaaccgccgc cgccccgagt tctgcaccag ggtcgtcgat
     2761 ccagccgatc cacaaccctc atgcagaaat cgcggaagaa agcgctcgcg aagaccgccg
     2821 cttaccgtga agccgtgctg accgcgatcg cgatcattcc gtcggcgccg gtgttggtgc
     2881 cgcaactcgt cggcaccgcg gccgacgagc tcgccgacct gcgtcaggcc gtgttcacgg
     2941 cggccggtgc gctgccggcg cgatggctgg ccgtcggcgt cggacccgcc gacgaggtgg
     3001 tcgggccgca ggccaccggc acgttcgccg ggtacggcgt cgacgtcagg gccgggttgt
     3061 cggccgacgc cgccgacgtc ggtgagttgc cgttgagcgc gctgatcacg gcctgggtgc
     3121 gggctgaggc ccgcccagcg gcgcgcgtcg aggtgcgggt gttcgaccgc gatctcagcg
     3181 ccgacgaggc gatacgacgc ggacgcgagc tccgggccgt tgtcgacgag gccgacgaac
     3241 cggtcggcgt gctcgtcgtc gccgacggcg ccaacacact gaccgagcgg gcccccggcg
     3301 gccacgaccc ggcctccgtc accgtgcaga tcgcgctgga cgacgcgctc gccgcgggtg
     3361 acgtcgccgg gctcgccgag ctgcccgcgt cgatcaccgg ccgcgtcgcg tggcaggtgc
     3421 tcgccggact ggccggtgcg ggcccgcggt cggccgagga gctgtaccgc ggcgcgccgt
     3481 acggcgtcgg ctacttcgcg ggcgtctgga ttccatgacc cggccaatcg cgattgtcgg
     3541 gccgaccggc accggcaagt cgcagctggc gctcggcgtc gccgaggctg ttggcggcga
     3601 gatcgtgaac gccgacgcga tgcagctcta ccgcggcatg gacatcggaa ccgccaagct
     3661 gcccgtcgac gagcggcgag gcattcccca ccaccagctc gacctgctcg atgtcaccga
     3721 gaatgcgacc gttgccaact accagcgcgg cgcggccgcc gacgtcgacg cgatcatggc
     3781 gcgcggcgcg gtgccggtca tcgtcggtgg atcgatgctg tacgtgcagt cgctgctcga
     3841 cgactgggcc tttcccgcga cggacgctgc ggtacgcgcc cgctgggaac agcggctcgc
     3901 gcacgtcgga gtggcgcagc tgcaccgcga gctggcggcg gtggacgccg ccgccgcgga
     3961 atcgatcctg cccaccgacg gccggcggat cgtgcgcgcg ctcgaggtcg tcgaactgac
     4021 cggccggccg ttcgcggcgt ccgcgccgac catcggcagc ccgcggtggg acaccgtgat
     4081 catcggatta gactgggaga caaaccaact cgacgaacgc ctggcgaagc gcaccgagca
     4141 gatgttcgac gcgggcctgg tcgacgaggt cgtcacgctc gtggacaaag gactgcgcga
     4201 cggtgtgacg gcgtcgcggg cgctgggcta cgcccaggtg atcgccgacc tcgacagtgg
     4261 cggcgatggc gcggccgcac gggagccgac gttcgtcgcg acgaggcggt acgtgcgccg
     4321 gcaacggtcg tggttccggc gcgatcaccg cattcactgg ctcgacggca gcgacgacag
     4381 caacgtcgag aacacgctgc aggcctggcg gcacgtatcc tgagcaggtg atcttcgcca
     4441 agggccacgg cacccagaac gatttcgtcg tgctgcccga catcgaggcg acgctgtcgc
     4501 tgaccccgga cgccgtcgcc gcgctgtgcg accgccgccg tggcctcggc gccgacggtg
     4561 tcctgcgcgt gaccaccgcc gacgccgcag tcgcccgggg tgtgctcgag cggctgcccg
     4621 agggagtgag cggacgcgac tggttcatgg actaccgcaa cgccgacggg tcgatcgcgg
     4681 agatgtgcgg caatggcgtg cgggtgttcg cgcactttct gcgggtcagc gagctcgagc
     4741 tgcgcgacga gttcgtggtc gggtcgctgg cgggaccgcg gccggtgcag gtgcacgggg
     4801 tcgacggcac gttcgcggac gtcaccgtcg agatgggcaa gaccaaccag ctcggcaccg
     4861 gcgaagcggt ggtcggcggg cgcaccttcc gcgggctggc cgtggacgtc ggcaatcccc
     4921 atctggcgtg cgtcgacccg gatctgagcg aggacgctct gagggctttg gacgtcgccg
     4981 cgccggtgtc gttcgaccgc acccagttcc ccgacggcgt caacgtcgaa gtggtgacgg
     5041 ccccggccga gggtgcggtg tcgatgcggg tacatgaacg cggtgtcggc gaaacccgtt
     5101 cgtgcggaac gggaaccgtc gccgccgtgg tcgcagccct ggcccacgac ggcgcgacga
     5161 cgggctcgct gacggtccgc atcccgggcg gcgaggtgga cgtcaccgtc accgccgcga
     5221 ccagttacct gcgcggcccg tcggttctgg tggcccgcgg cgagatctcg gaggaatggt
     5281 ggcgggccca gtgacgttaa ttcagatgcg cgtccgacgt tcggcgtgca ccatcactt
//
LOCUS       DASWBF010000484         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_783697,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000484 DASWBF010000000
VERSION     DASWBF010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783697"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VF874_17850"
     CDS             complement(<1..209)
                     /locus_tag="VF874_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177851.1"
                     /translation="MPAVSSRSFPVATPLSGGLPGKNERPHSLAARTSGTRSIIMSPD
                     PRPWTQIRLGAGTRNDIDICPTLGGG"
     gene            complement(245..1297)
                     /locus_tag="VF874_17855"
     CDS             complement(245..1297)
                     /locus_tag="VF874_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9177852.1"
                     /translation="MTIVGGFDVHRQQITFDYVDNDGLVRWGQIRPATRKTLRRWLSE
                     HCPAGDGEFALEGCTGWRYVVEELAAAGVVAHLGDPAEIAALRGPKKRAKTDRADARL
                     LRTLLVEGRFPESWIPPAHVLEVRTLGRLYCTLMDERRAWQQRIHAQLFHQGCPPIRA
                     LLSEPGRAALAHAELSPAGRQYVDTALRRIDELSAEIEPLRTQLVGFARRQRGCQALQ
                     RQYGIGWLCAAIIWAEIGDARRFSSSDQLVRFAGLDITVYSSDGKRSPGHLSRQGSPQ
                     LRWAAFEAAKCAARRGSPDHAYYHTLAAKHDGHNGKNPTLAVERKILHRCYHTLRELG
                     DAALALPDTPAQEVAA"
     gene            1365..1706
                     /locus_tag="VF874_17860"
     CDS             1365..1706
                     /locus_tag="VF874_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HEX9177853.1"
                     /translation="MRGLPETDVARVQRWCRDKVPAHVRDQVRVEVDVAERHLTIVEC
                     RPPWRADMGAEWTRFPIARLRYTKTTGVWSLYWRDRNLRFHAYDPVSPTASIEELLAE
                     VERDPTAIFWG"
     gene            complement(1721..2917)
                     /locus_tag="VF874_17865"
     CDS             complement(1721..2917)
                     /locus_tag="VF874_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021754435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEX9177854.1"
                     /translation="MRKTRHGEMVVEKQERFARLIGQGVSNSEACRIVGINRRTGTRW
                     RYGRTILNTAGESVHYPPVSLAEPKERHPRYLSLAERTMMADERRAGVSVRGIAQQMG
                     RAASTVSRELRRNADDQGRYLPATAQRLCNQRQSRSSRTRRLARDEQLYGVVAELLGK
                     RWSPEQIAHELPMRFPNQPERHLCTESIYQAIYDPRIALTRPAKRRRRRRRRRVQGLE
                     RRGRLTAMTMIADRPAEVEDRVQVGHWEGDCIMGAGNRSAIGTLVERVTRFLILVHVP
                     TRRPTAEAMRDGVAVALGALAPHLRRTLTWDQGKELSLHQQITARIGTRVFFCDAHSP
                     WQRGSNENTNGLLRDYFPKGTDLANISPEELQRVADEINNRPRKTRGWARPADLITDA
                     VTVRTA"
     gene            2939..>3178
                     /locus_tag="VF874_17870"
     CDS             2939..>3178
                     /locus_tag="VF874_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX9177855.1"
                     /translation="MASVLRRSLESAPVTASLAFSAHEVLEAAWKNSADDERMLWQGL
                     AQLAVGLTHARRGNARGAVTLLHRGTQRIRTRPPGG"
BASE COUNT          514 a         1064 c         1057 g          543 t
ORIGIN      
        1 cctcccccca acgtggggca gatgtcgatg tcgttgcgcg tgccggcgcc cagccttatc
       61 tgggtccacg gacgagggtc cggcgacatg atgattgatc gggtgcccga cgtgcgggct
      121 gctaaagagt gcggccgctc attctttccg ggaagtccac ccgacagcgg agtggcgaca
      181 ggaaaactgc gggagctgac cgcaggcatc agctgtatgg gcagggcacg tgcgcaacgc
      241 atggttaggc ggccacctcc tgcgccgggg tgtctggcag cgccagggcg gcatcgccga
      301 gctcgcgcag ggtgtgatag cagcggtgca ggatcttgcg ctccaccgcc aaggtggggt
      361 tcttgccgtt gtggccgtcg tgtttggcgg ccagcgtgtg gtagtaggcg tgatccgggg
      421 aaccgcggcg cgcggcgcat ttggccgcct caaacgccgc ccagcgcagc tgcggtgagc
      481 cctgccgcga caggtgcccc ggtgagcgtt tgccgtcgga ggagtagacg gtgatatcca
      541 gcccggcaaa ccgcaccagc tgatcggagc tggaaaaccg gcgggcatca ccgatctcgg
      601 cccagatgat cgccgcgcac agccagccga tgccgtactg gcgctgcagc gcctgacagc
      661 cgcgttggcg tcgcgcgaag ccgaccaatt gggttcgcag cggctcgatc tcggcgctga
      721 gctcatcaat gcggcgtagc gcggtgtcca cgtactggcg tcccgccggc gacagctcag
      781 cgtgcgcgag cgccgcgcgg cccggttcgg ataacagcgc tcgaatcggt gggcagccct
      841 gatggaacaa ctgggcgtgg atgcgctgct gccaggcgcg ccgctcgtcc atcaaggtgc
      901 agtacaaccg gcccagcgtg cggacctcca gcacgtgcgc cggcgggatc cacgactccg
      961 ggaaccggcc ctccaccagc agcgtgcgca gcaaccgggc atcggcgcgg tcggtcttgg
     1021 cgcgcttctt gggaccccgc aacgccgcga tctcggccgg atcacccaga tgcgccacca
     1081 cccccgccgc ggctagctcc tcgaccacat accgccaccc ggtgcagccc tcgagcgcga
     1141 actcgccatc accggccggg cagtgctcgc ttagccagcg ccgcagcgtc ttccgcgttg
     1201 ccggacggat ctggccccac cggaccaacc cgtcgttatc cacatagtcg aacgtgatct
     1261 gctgccggtg cacgtcaaac ccgcctacaa tcgtcattgg gacctcctca agctccggcg
     1321 gattccagtg aacgtcgcaa caggacagtt gcaacactag tggggtgagg ggtctgccgg
     1381 agacagatgt tgcgcgggtc cagcggtggt gccgggacaa ggtgcctgcg catgtccgtg
     1441 accaggtgcg agtcgaggta gacgttgctg agcgtcatct gacgatcgtg gagtgccggc
     1501 cgccgtggcg ggcggatatg ggcgcggagt ggactcggtt cccgattgcg cggctgcgct
     1561 acacgaagac caccggcgtg tggtcgttgt attggcgcga ccgcaacttg cgtttccacg
     1621 cctatgaccc ggtgtcgccc acggccagta tcgaggagtt gttggctgag gtggaacgcg
     1681 atcccacagc gatcttttgg ggctgaccag ccggtgtcgc ttatgcggtc ctaaccgtaa
     1741 cggcatcggt gatcaggtcg gccggtcggg cccagccgcg agttttgcgt ggccggttgt
     1801 tgatttcgtc ggcgacccgc tgcagttcct cgggcgagat gttggccagg tcggtgcctt
     1861 tgggaaagta gtcccgcagt aggccgttcg tgttctcatt ggagccgcgc tgccacggcg
     1921 agtgtgcgtc acagaagaac acgcgtgtgc cgatgcgggc ggtgatctgt tggtgcagcg
     1981 aaagttcctt gccttgatcc caggtcagcg tccgccgaag atggggggca agagcgccca
     2041 gtgcgacggc gacgccgtca cgcatcgcct cggcggtcgg tcgcctcgtg ggcacgtgga
     2101 ccaggatcaa aaagcgagtt acgcgttcga cgagagtgcc gatcgccgaa cggtttccgg
     2161 cacccatgat gcagtcgccc tcccaatgcc ctacctgcac tcggtcttca acctcagcgg
     2221 gtcgatcagc gatcatcgtc atagcagtga gccgaccacg gcgttctagc ccctggacgc
     2281 ggcggcggcg tcgccgacgc cgccgtttgg ccggacgggt caaagcgatc cgcggatcat
     2341 agatcgcctg gtaaatcgat tcggtacaca ggtgccgctc gggttggtta gggaaccgca
     2401 tcggcagctc gtgggcgatc tgctcaggac tccaccgctt gcccaacagc tcagcaacga
     2461 caccatagag ctgctcgtca cgcgctaagc gccgggtgcg cgatgaccgg gattgccgct
     2521 ggttgcacag ccgctgcgcc gtcgccggca agtagcggcc ctgatcgtca gcgttgcggc
     2581 gaagctcccg tgacaccgtc gaggccgccc tgcccatctg ctgggcaatg ccgcgcacac
     2641 tgacacccgc acggcgctcg tcagccatca tcgtccgctc ggccagcgac aaataccgag
     2701 gatgccgctc cttgggttct gcaagactca ccggtggata gtgcaccgac tcgccggccg
     2761 tgttcaggat tgtgcgccca tagcgccagc gtgtccctgt cctgcggttg attcccacga
     2821 tccggcacgc ctcggagttg ctcaccccct gcccaatcag ccgagcgaac cgctcctgct
     2881 tctccacgac catctcgccg tgtcgggtct tgcgcacagc gcccaccgca acctccacat
     2941 agcctcggtg ttgcgacgtt cactggaatc cgcccctgtc acggccagcc tggcattcag
     3001 cgcgcacgag gtgcttgagg ccgcatggaa gaacagcgcg gacgacgagc ggatgctgtg
     3061 gcagggcctg gcccagctcg cggtcggcct gacccacgcc cgccggggta acgcccgcgg
     3121 cgccgtcacc ctgctccacc gcggcaccca gcgcatccgc acccgtccgc cgggcggg
//
LOCUS       DASWBF010000485         9583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_784308,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000485 DASWBF010000000
VERSION     DASWBF010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9583
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784308"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VF874_17875"
     CDS             <1..593
                     /locus_tag="VF874_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX9177856.1"
                     /translation="QGSVLLDGEDVYGPGVDPVSVRKTVGMVFQRPNPFPTMSIRDNV
                     VAGLKLQGVRSRKILDEVAERSLKGANLWNEVKDRLDKPGGGLSGGQQQRLCIARAIA
                     VQPDVLLMDEPCSALDPISTLAIEDLIAELKMDFTIVIVTHNMQQAARVSDHTAFFNL
                     EAAGKPGRLVEIDDTEKIFSNPSQKATEDYISGRFG"
     gene            complement(643..1311)
                     /gene="phoU"
                     /locus_tag="VF874_17880"
     CDS             complement(643..1311)
                     /gene="phoU"
                     /locus_tag="VF874_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX9177857.1"
                     /translation="MRTAYHEQLDALTAQLGEMCGMAGVAMERATQALLQADLVLSEQ
                     VITDHEQITGMSLRAEEAAFVLLALQAPVAGDLRAIVSSIQIVADVERMGALALHVAK
                     IARRRHPQPALPEEVNGYFAEMGRVAVELGNAAQEVVVSRDPEKAARLREEDDAMDDL
                     HRHLFTVLMDREWKHGVAAAVDVTLLGRFYERFADHAVEIGRRVIFQATGKLPEEDGL
                     PTSN"
     gene            complement(1392..3452)
                     /locus_tag="VF874_17885"
     CDS             complement(1392..3452)
                     /locus_tag="VF874_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX9177858.1"
                     /translation="MRDGDNATPSHRRGSGDHKSDPWANQWLTRKPRPSTVAAPWERL
                     TVSPPTPDPGPDDDHDSGGSHTDGVPVADLIAKIGTVQNGRRSSRHRVDDEPDEQLAD
                     TGLHAEVTARIPIVTMPEMPDLAAARHRKADGLLHGDANGRTTMPAADNRPPIHAVQV
                     QPAPKRRRPFVMAGRGAAAVLSILALVLTGAAWQWSTSKNNRLNLISALDPDSRDIVD
                     PKAQFGDENFLIVGVDSRFGQNADMGAGDTEEAGGARSDTVMLVNIPANRKRVVAVSF
                     PRDLAIKPMLCHAWDPQTGEYGPVYDEDTGTYGPEQVYTETKLNSAYSFGGPKCLVKV
                     IQKLSGLAINRFMAIDFAGFARMVDALGGVKVCSTTALEDYELGTVLPEAGWQTIDGR
                     TALNYVRARQVTTETNGDYGRIKRQQLFLSSLLRSLISNEVFFSLKKLNDVVNTFIND
                     SSVDNVDTKDLVDLGQSIQGMSAGRITFLTVPTGETDSEGNEPLRTEDNRALFDAIIN
                     DDPLPGENNQNETTTPTTATNSPLASSSAPTDAASHDTGELVDAVTTEPKNITVHVSN
                     STGQNGLGASAASELEQHGFIVTTPDDYPSALPSTTVFFSSGNEQAAATVASAFPNPQ
                     MERVTGMGDVVQVVLGQDFYTVTAPPPSGSSVHVHLTHNIHTTPTPLPQDLSITNGAD
                     VSCE"
     gene            complement(3644..4804)
                     /gene="dusB"
                     /locus_tag="VF874_17890"
     CDS             complement(3644..4804)
                     /gene="dusB"
                     /locus_tag="VF874_17890"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX9177859.1"
                     /translation="MLPAALAPGLRIGHIALRSPVVLAPMAGVTNVAFRTLCRELEVA
                     RAGTVSGLYVCEMVTARALVERHPVTMHMVSFAPDEAPRSLQLYTVDPDTTYAAARMI
                     ADEGLADHIDMNFGCPVPKVTRRGGGAALPIKRRLFGQIVAAAVRATDGTGIPVTVKF
                     RIGIDDAHHTHLDAGRIAEQEGAAAVALHARTAAQRYSGTADWAQIAALKQHVRSIPV
                     LGNGDIFDADDAVAMMATTGCDGVVIGRGCLGRPWLFAELSAALTGAPAAAPPTLGEV
                     AQIIRRHGALLTAHFGEDKGMRDIRKHIAWYLHGFPAGADLRRALALVKTLKELDVLL
                     DRLDPDVPFPFAATGPRGRQGSPASVALPDGWLSDPDDCVVPVGADVMHSGG"
     gene            complement(4804..5802)
                     /locus_tag="VF874_17895"
     CDS             complement(4804..5802)
                     /locus_tag="VF874_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX9177860.1"
                     /translation="MTQNLTDVHLLHELEPVAERYLNRHLTMSKDWNPHDFIPWSDGK
                     NFYALGGQDWHPEESKLSDVAQVAMVQNLLTEDNLPSYHREIAMNFSMDGAWGTWVNR
                     WTAEENRHGIALRDYLVVTRSIDPVELEKLRVEQMTRGFSPGQNQQGDLFAESLFDSV
                     VYVTFQELATRISHRNTGKACNETVADQLLARVSADENLHMIFYRDVSEAGLEIAPNQ
                     AMKSLHRVLRNFKMPGYTVPEFRRKAVIIAVGGVYDPRVHLDDVVMPVLKKWRIFERE
                     DFTGEAARMRDDLALLVEELEEACDKFEESKRRRLEREARKAEKLTAKRALASARA"
     gene            5989..6459
                     /locus_tag="VF874_17900"
     CDS             5989..6459
                     /locus_tag="VF874_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177861.1"
                     /translation="MRKLIVIGAGAVGAAGVAMALVGQGVAGADALSDVTGKYYGQAK
                     SVLSGAGLTPIVATRVGDQATEDECVIDRIQNANFLNGTGSAASGTVYVYLNCYANVA
                     SPQQAGYSRQSELGKAAYEAQQQAALEAQQQAEAAAAAQQGAESEQNLTAENTR"
     gene            complement(6456..7067)
                     /locus_tag="VF874_17905"
     CDS             complement(6456..7067)
                     /locus_tag="VF874_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9177862.1"
                     /translation="MSARAKSRHQGRWSGVPIEDRQALRRDGLVAAGVTLLGGEGGPA
                     LTVRAVCREAGLTERYFYESFSDRDEFVRAVYDDVCTRAMAALMSATTAREAVERFVA
                     LMVDDPVRGRVLLLAPEVEPVLVRSGAEWMPSFIKLLQRQLTTISDPALQNMVATGLI
                     GALTALFTAYLDRRLTATREQFIDYCVAMLLSRASYPESSSAT"
     gene            7192..8202
                     /locus_tag="VF874_17910"
     CDS             7192..8202
                     /locus_tag="VF874_17910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX9177863.1"
                     /translation="MTQDTSEAHPLTSEAPTTGCPVAPGGYDAPPEPLGPDSLTWKYF
                     GDWRGMLQGPWAGSMQNMHPQLGAAVEEHSTFPREPWPRVLRSLYPIGGVVFDGDRAP
                     ITGAQVRDYHTDIKGTDTKGRRYHALNPDVFYWAHSTFFMGTIHVAERFRGGITEAEK
                     RQLFDEHIAWYRMYGMSMRPVPETWEEFQVYWDRMCREVLEDNKATRDVLDLAHYPKP
                     WFAQWVPDLLMTPLQQHVVAPFMVWLCVGLYDEPVRELLGYSWSRRDEWLHRRFGDAV
                     RLAFSLLPPRYRKHPRARAGLDRASGRIPHDAPLPQTAARNLPPLDERHKPTHYCPVA
                     GV"
     gene            8232..9401
                     /locus_tag="VF874_17915"
     CDS             8232..9401
                     /locus_tag="VF874_17915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9177864.1"
                     /translation="MKAPELLGGRYELRGVLGRGGMAEVRDGWDTRLARPVAIKILHP
                     AIDTQPGNRRRFEAEARTAAALDCSHIVAVHDSGEHNGMPFIVMERLSGESLHDKIMR
                     GPLPQPLVRSVLDDVLAALSAAHDGGILHRDIKPANILFTTSGDAKVSDFGLAKTAQS
                     NLTATGQIFGTMAYLSPDRALGKPATAADDVYAVGVLGYEALTARRPFLQDNIAALAQ
                     AILHEQPPPIAVLRPDVDRALAAVIERAMARDPAMRFPSARAMRAALGTHAPPAPPPG
                     TQVLSTPLPPPMPVAPLAAVRHPSRRNTLLALGAVFAALLLGVIVIAVDSSQTGSPEP
                     VTTSTTVAPPPPSTPPLATTAIRPPTATASIVVPEKPPPGRNKPGKKGDGNGEGG"
BASE COUNT         1566 a         3102 c         3219 g         1696 t
ORIGIN      
        1 tgcagggttc ggtgctgctc gacggcgagg acgtctacgg cccgggggtc gatccggtga
       61 gcgtgcgtaa aaccgtcggt atggtttttc aacgcccaaa tcccttccct accatgtcga
      121 ttcgcgacaa tgtggttgcc ggcctgaagc ttcagggagt acgtagccgc aagattctcg
      181 acgaggtcgc cgagcgctca ctcaaaggcg cgaacctgtg gaacgaagtc aaggaccgac
      241 tcgacaagcc ggggggcggg ctatcgggcg gtcagcagca gcggctgtgc atcgcccgtg
      301 caatcgcggt gcagcccgat gtgctgctga tggacgagcc gtgctcggcg ctggacccga
      361 tctccacgct ggcaatcgaa gacctgatcg ccgagttgaa gatggatttc acgatcgtca
      421 tcgtcacgca caacatgcag caggccgcgc gggtgagcga ccacaccgcg ttcttcaacc
      481 tggaggccgc gggaaagccc ggtcggctgg tcgagatcga cgacaccgag aagatcttct
      541 ccaacccgag tcagaaggcg acggaggatt acatctccgg tcgcttcggc tgaccaccat
      601 gcgttgactc tgcgtcctgg gcgcgaaagt tcgagtgtcc cgtcagttgc tcgtgggcag
      661 gccgtcctct tcgggcagct tgccggtggc ctgaaagatc acccgtcgac cgatctcgac
      721 cgcgtggtcg gcgaaccgct cgtagaaccg tcccagcagc gtcacgtcga ccgccgcggc
      781 gaccccgtgt ttccactcgc ggtccatcag cacggtgaaa aggtggcggt gaaggtcgtc
      841 catcgcgtcg tcttcttcgc gcagccgggc ggctttctcc gggtcgcggg agaccaccac
      901 ttcctgcgcg gcattaccca attcaactgc tactcttccc atttcggcga agtaaccgtt
      961 gacctcttcg ggcagcgcgg gctggggatg cctgcgacgg gcgatcttgg cgacgtgtag
     1021 cgcaagagcg cccatccgtt cgacgtcggc gacgatctga atcgagctga cgatggcgcg
     1081 caggtccccg gcgaccggcg cctgcagcgc cagcaacacg aacgcggctt cttcggcccg
     1141 gaggctcatc ccggtgattt gctcgtggtc ggtgatgact tgttcggaca gcaccagatc
     1201 ggcttgcagc agcgcctgag ttgcacgttc catcgcgaca ccggccatgc cgcacatctc
     1261 accgagctgt gcggtcaggg cgtcgagttg ctcgtggtac gcggtccgca tgtttccaca
     1321 ctacgttctg acgtctggag aggtcacgag ccgcgggtga actcgcggtg aacggccgag
     1381 aatcgctgtc gttattcgca gctgacgtcg gcgccgttgg tgatgctgag atcttgcggc
     1441 aatggggtgg gtgtggtgtg gatgttgtgc gtcaggtgca cgtgcactga cgagccactc
     1501 ggcggcgggg cggtgacggt gtagaaatct tggcccagca ccacctgaac gacgtcaccc
     1561 atccctgtca cccgctccat ctgaggattg gggaacgctg acgccacggt cgccgccgcc
     1621 tgctcgttgc ccgacgagaa gaacacggtg gtcgacggca gcgcgctcgg gtagtcgtct
     1681 ggcgtggtga cgatgaagcc gtgctgctcc agctcgctgg ccgcgctcgc accgagaccg
     1741 ttctggccgg tggagttgga gacgtgcacg gtgatgttct tcggctcggt ggtcaccgcg
     1801 tcgaccagtt cgccggtgtc gtgggacgcg gcatcggtcg gggcgctgct gctggcgagc
     1861 ggtgagttgg tggccgttgt gggagtggtg gtttcgttct ggttgttctc gccgggcagc
     1921 ggatcgtcgt tgatgatcgc atcgaacagc gcccgattgt cctcggtgcg cagcggctca
     1981 ttgccttcgg agtcggtttc accggtcggg acggtgagga acgttattcg cccggcggac
     2041 atgccctgga tcgactggcc caggtcgacg aggtccttgg tgtcgacgtt gtcgacggag
     2101 ctgtcgttga tgaacgtgtt gacgacgtcg ttgagcttct tgaggctgaa gaacacctca
     2161 ttggagatca gtgagcgcag caacgacgac aggaacaact gctggcgctt gatgcggccg
     2221 tagtccccgt tggtctcggt ggtgacctgc cgggcgcgca cgtagttcag cgcggtgcgg
     2281 ccgtcgatgg tttgccaacc ggcctccggc agcaccgtgc ccagctcata gtcctccagc
     2341 gcagtggtgc tgcacacctt cacgccgccg agcgcgtcga ccatcctcgc gaaacccgcg
     2401 aagtcgatcg ccatgaaccg gttgatcgcc agccccgaca gcttctggat caccttgacc
     2461 agacacttgg gtccaccgaa cgaatacgcc gaattgagct tggtttcggt gtaaacctgc
     2521 tctgggccgt aggtgccggt gtcctcgtcg tacaccggtc cgtactcgcc ggtctgcgga
     2581 tcccacgcgt gacacagcat cggcttgatt gccagatcgc gcggaaacga cacggccaca
     2641 acacgtttgc ggttggccgg gatattgacc agcatcacgg tatccgagcg ggcaccgccg
     2701 gcttcctctg tgtcaccggc acccatgtcg gcgttctggc cgaaccggct gtcgacgccg
     2761 acgatgagga agttctcgtc accgaactgg gccttcgggt cgacaatgtc gcgcgagtcc
     2821 gggtccagcg ccgagatcaa gttcagcctg ttgttcttcg aggtgctcca ctgccacgcc
     2881 gcgccggtca gcaccagcgc gaggatggac agcacggctg cggcgccacg gccggccatg
     2941 acgaagggtc gacggcgttt cggtgccggt tgaacctgga cggcgtggat cgggggccgg
     3001 ttgtcggcgg ccggcatagt cgtccgaccg ttcgcgtcgc cgtgcagcag cccgtcggcc
     3061 ttgcggtgac gggccgcggc cagatccggc atctccggca tggtgacgat cgggatccgc
     3121 gctgtgacct cggcgtgcaa gccggtgtcg gcgagctgct catccggctc gtcgtcgacg
     3181 cgatgccgcg acgaccgtcg cccattttga accgttccga ttttggcgat gaggtcggca
     3241 acgggcactc catcggtgtg gctgccgccg gagtcgtgat cgtcgtcggg ccccggatcc
     3301 ggtgtgggcg gcgaaactgt cagccgttcc cacggcgcgg ctaccgtcga cggccgcggt
     3361 tttcgggtaa gccattggtt ggcccaggga tcagacttgt ggtcgccaga gccgcggcgg
     3421 tgactaggag tggcgttgtc gccgtctctc atgtcctacc ggcctccgac tcaaaagtgg
     3481 tacagcccgt ccgcaaccgt gtgctcacga tccgctatct gtctacgcgc agcactatgc
     3541 cttttaacag gccaaggcat gcatccgcca cctgagcgag atgcagccag agcaacatcg
     3601 tacttagaca ttctgagcac cgtgatgtcg gaaccgtctc ggttcagcct ccggagtgca
     3661 taacgtccgc gccgacgggc acgacgcagt cgtcgggatc ggataaccag ccatcgggca
     3721 gcgccacgga agctggcgaa ccctgccgcc cccgtggccc cgtcgcggcg aaggggaacg
     3781 ggacgtccgg atccaatcga tcgagcagga cgtccagctc tttcagggtc ttcaccagcg
     3841 ccaatgcgcg tcgcaagtcg gctcccgcgg gaaaaccatg caggtaccag gcgatgtgtt
     3901 tgcggatgtc tcgcatgcct ttgtcttcac cgaagtgagc ggtcagcagc gcgccgtgcc
     3961 gtcggatgat ctgcgcgact tcgccgagtg tgggcggcgc ggcggccgga gcgccggtca
     4021 gcgcagccga cagttcggcg aacaaccacg gccggcccag acagccgcgt ccgatcacga
     4081 cgccgtcgca gccggtggtg gccatcatcg cgaccgcgtc gtcggcgtcg aagatgtcgc
     4141 cgttgcccag caccgggatc gaccgaacat gctgcttgag cgccgcgatc tgcgcccagt
     4201 cggccgtacc tgagtaccgc tgggcagcgg tgcgggcgtg caacgccaca gcggccgcgc
     4261 cctcctgctc ggcgatccgc ccggcgtcca ggtgggtgtg gtgcgcgtcg tcgatcccga
     4321 tccggaactt cacggtcacc gggatcccgg tgccgtcggt ggccctcacg gcggcggcga
     4381 cgatctgccc gaatagccgg cgtttgatcg gcagggcggc gccaccaccg cgtcgtgtga
     4441 ctttcggcac aggacagccg aagttcatgt cgatgtggtc ggcgaggccc tcgtcggcga
     4501 tcatccgcgc tgcagcgtag gtggtatccg ggtcgacggt gtacagctgc agtgatcggg
     4561 gcgcttcatc gggcgcgaac gaaaccatgt gcatggtcac ggggtgccgc tcgaccaaag
     4621 cgcgggcggt caccatctcg cagacgtaca gcccgctgac cgtgccggcc cgggcgacct
     4681 cgagttcgcg gcagagcgtg cggaacgcga cattggtcac gccggccatc ggcgccagga
     4741 ccaccgggct gcgcagcgca atgtgcccga tgcgcagacc cggggccagt gcggccggaa
     4801 gcattacgcc ctagccgacg ccagcgcccg cttggcggtg agcttctcgg ccttgcgggc
     4861 ctcgcgctcg aggcgccggc gcttggactc ctcgaacttg tcgcaggcct cttcgagctc
     4921 ctcgaccagc agtgcgagat cgtcacgcat cctcgcggct tcgccggtga agtcctcacg
     4981 ctcgaagatc cgccacttct tcagcaccgg catcaccacg tcgtccaggt ggacgcgcgg
     5041 gtcgtagacg ccgccgacgg cgatgatcac ggccttgcgg cggaactccg gaacggtgta
     5101 gccgggcatc ttgaagttgc gcaacacgcg gtgcagcgac ttcatcgcct ggttcggcgc
     5161 gatctccagg cccgcctcgg acacgtcgcg gtagaagatc atgtgcaggt tctcgtcggc
     5221 ggacacccgg gcaagcaact ggtcggccac cgtttcgttg caggccttgc cggtgttgcg
     5281 gtgcgagatg cgggtggcga gttcctggaa ggtgacgtag acgacggagt cgaacaggct
     5341 ctccgcgaac aggtcaccct gctggttctg gccggggctg aacccgcggg tcatctgctc
     5401 gacgcgaagc ttctccagct cgaccgggtc gatcgatcgg gtgacgacga ggtagtcccg
     5461 cagcgcgatg ccgtgccggt tctcctcggc ggtccagcgg ttcacccagg tgccccaggc
     5521 gccgtccatg ctgaagttca tcgcgatctc gcggtgatac gacggaaggt tgtcctccgt
     5581 cagaaggttc tgcaccatcg ccacctgggc gacgtccgac agcttcgact cctcgggatg
     5641 ccagtcctga ccgccgagcg cgtagaagtt cttgccgtcc gaccacggga tgaagtcgtg
     5701 cggattccag tccttggaca tggtcaggtg ccggttgagg taccgctcgg ccaccggttc
     5761 aagctcgtgc aagaggtgca cgtcggtcag gttttgagtc atggccccgt ccagttatct
     5821 gtacctgttg gttgcactca atataagtgt gtacgccggt gacattcaag ttcccacgcc
     5881 attggcgtaa tgcgcggacg cggcgcgggc gataatatcc gcgtggggca tccgctctgc
     5941 tgaacactta atcacgctcg ccgcagggaa agacggaggt cgtcagacgt gaggaagctc
     6001 attgttatcg gtgcgggcgc agttggcgcc gcgggcgtcg cgatggcgtt ggtcggccag
     6061 ggtgtcgcgg gtgccgacgc gttgagcgac gtgactggca agtactacgg ccaggccaag
     6121 agcgtgctgt cgggggcggg gctgacgccg atcgtggcga cgcgggtggg cgaccaggcg
     6181 accgaggacg agtgcgtcat cgaccggatc cagaacgcca acttcctcaa cggcaccggc
     6241 agcgccgcgt ctggcaccgt ttacgtgtac ctgaactgct acgccaatgt ggcctcgccg
     6301 cagcaggcgg gctattcgcg gcagtctgag ctgggcaagg ccgcctatga ggcgcagcag
     6361 caagcggcgc tagaggcgca acagcaggcg gaggcggcgg ccgccgcgca gcagggggcg
     6421 gagtccgagc agaacctgac cgccgagaac acgcgctagg tcgccgacga actctcgggg
     6481 tagctcgctc tgctcagcag catcgcgacg cagtagtcga tgaactgctc gcgggtggcc
     6541 gtcaaccggc ggtccaggta ggcggtgaac agcgcggtca gcgcgccgat caacccggtg
     6601 gccaccatgt tctgtagcgc cgggtcgctg atcgtggtga gttggcgctg cagaagcttg
     6661 atgaagctcg gcatccactc ggctcccgac cgcaccagaa ccggctccac ctccggcgcc
     6721 agcagcagca cccggccgcg cacggggtcg tcgaccatca gcgcgacgaa ccgctcgacg
     6781 gcttcgcgtg cggtcgtggc cgacatcaac gccgccattg cacgggtgca gacgtcgtcg
     6841 tacacggcgc gcacgaattc gtctctgtcc gagaagcttt cgtagaagta gcgttcggtc
     6901 aacccggcct cgcgacacac cgcccgcacg gtcagggcgg gaccgccttc gccgccgagc
     6961 agggtgacgc cggcggccac gagtccgtcg cggcgcagcg cctggcggtc ctcgatcgga
     7021 acgcccgacc agcgaccctg atgtctgctc ttcgcgcgag cgctcatcgc tgatgtctgc
     7081 tcttcgcgcg agcgctcatc gctgctgttg accggtcgac acgactctcc tagacttgat
     7141 tctgacaacg cacgtagtca attgactttg gcgctgatgg gaccgtcacc agtgactcaa
     7201 gatacgtccg aggctcaccc gctgaccagc gaagccccga ccaccggctg cccggtggcc
     7261 ccgggcggct acgacgcacc gccggaaccg ttgggtcctg actcgctgac gtggaagtac
     7321 ttcggcgact ggcgggggat gctgcaaggc ccgtgggcag ggtcgatgca gaacatgcac
     7381 ccgcagctcg gcgctgccgt cgaagagcac tcgacgtttc cccgcgagcc gtggccgcgg
     7441 gtgctgcggt cgctgtaccc gatcggtggc gtggtgttcg acggcgaccg cgcgccgatc
     7501 accggcgcac aggtacgcga ctaccacacc gacatcaagg gcaccgacac gaagggccgt
     7561 cgctaccacg cgctgaaccc ggacgtcttc tattgggcgc attcgacgtt cttcatgggc
     7621 acgatccatg tggccgagcg gttccgcggt gggatcaccg aggccgagaa gcgtcagctg
     7681 ttcgacgagc acatcgcgtg gtaccggatg tacgggatga gcatgcgtcc ggtacccgag
     7741 acgtgggaag aattccaggt gtattgggac cggatgtgtc gcgaggtgct ggaggacaat
     7801 aaggcaaccc gcgacgtcct cgacctcgcg cactacccga aaccgtggtt cgcacaatgg
     7861 gttccagatt tgctgatgac gccgttgcag cagcacgtag tcgcgccgtt catggtgtgg
     7921 ctgtgcgtcg ggctgtacga cgagcccgtg cgtgagttgc ttgggtacag ctggtcgcgc
     7981 cgcgacgagt ggctgcaccg caggttcggc gacgcggttc ggctggcgtt ctcactgctg
     8041 ccccctcgat atcgcaagca tccgcgtgcc cgcgcagggc tggatcgggc cagcggccgc
     8101 atcccgcacg acgccccgtt gccacagacg gcggcccgca atctgccgcc gctcgacgag
     8161 cggcacaagc caacccacta ttgccccgtc gccggtgttt agctgaacgg cgggcggtta
     8221 acgtaggcag ggtgaaggcc cccgagctgc tcggtggccg ctacgagcta cgtggcgtgt
     8281 tgggtcgtgg cggtatggcc gaggtccgcg acggctggga cacccggttg gcccgtcccg
     8341 tcgcgatcaa gattttgcac ccggcgattg acactcaacc gggcaatcgc agacgtttcg
     8401 aagccgaagc gcgcaccgcc gcggcgctgg actgctcgca catcgttgcg gtacacgaca
     8461 gcggcgagca caacgggatg ccgttcatcg tgatggagcg gctctccggc gagtcgctgc
     8521 acgacaagat catgcgcggt ccgctgccgc agccgctggt gcggtcagtc cttgacgacg
     8581 tgctcgccgc attgtccgcg gcacacgacg gggggattct gcatcgcgac atcaagccgg
     8641 ccaacatctt gttcacgacg tccggcgatg cgaaagtcag cgacttcggt cttgcgaaga
     8701 ccgcccagag caacttgacg gccaccgggc agatcttcgg gacgatggcg tacctgagcc
     8761 ccgatcgggc gctcggcaag ccggcgaccg ccgccgatga tgtgtatgcg gtgggcgtct
     8821 tgggatacga ggcgctgacc gcgcgccgac ccttcttgca ggacaacatc gccgcgttgg
     8881 cacaggcgat cctgcacgag cagccgcctc ccatcgccgt gcttcgtccc gacgtcgatc
     8941 gcgcgctggc cgcagtgatc gaacgggcca tggcccgtga tccggcgatg cggttcccca
     9001 gtgcccgggc aatgcgcgcg gccctcggga cgcatgcgcc tccggctccg ccgcccggga
     9061 cgcaagtgct cagcacgccc ctgccgccgc caatgccggt ggcgccgttg gccgccgtgc
     9121 gacatccgag ccgacgcaac accctgctgg cgttgggcgc ggtgttcgcg gctctgctgc
     9181 tgggggtgat cgtgatcgcg gtcgactcat cgcagacggg gtcgcccgag cccgtcacca
     9241 ccagcacgac ggtggcgccc ccgccaccga gcacgcctcc cctggccact acggcgattc
     9301 ggccgccgac ggcgaccgcc agcatcgtgg tgccggagaa gccgccgccg gggcgcaaca
     9361 agcccggcaa gaaaggcgac ggcaatggcg agggcggcta gggcgagata acctctcgca
     9421 caaggctttt caggccgttt tcagtctaag gcggcagact aacgcaacgc cgcatcgccc
     9481 tagccgctcg ccgttatccc cctgcgaggg tcaggagatc ctgcaactga ccgttcccga
     9541 ggtttccgag actcccgagg cctcccaggt tgccgatgtt gcc
//
LOCUS       DASWBF010000486         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_636159,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000486 DASWBF010000000
VERSION     DASWBF010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636159"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..521
                     /locus_tag="VF874_17920"
     CDS             213..521
                     /locus_tag="VF874_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177865.1"
                     /translation="MEMSFAVLDPTALAPSDEQIAEAILAALPADGAPVLWTRIRTRL
                     PGSHWSRETTLLRLHTCGEVRLRKFQRALFVSLGDDVSRETACRARAGGRVLQASVVA
                     "
     gene            complement(778..1167)
                     /locus_tag="VF874_17925"
     CDS             complement(778..1167)
                     /locus_tag="VF874_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9177866.1"
                     /translation="MTDLQTLKRRVVQGVQRRLVNPIGRQLPITMIETTGRKSGQPRR
                     TAVGGRMLDNQFWFVSEHGEHSHYVHNIKANPKVRVRVGGQWRTGTAHLLPDDDPIAR
                     LGELPTLNSAVVRLMGSDLLTIRVDLD"
     gene            complement(1276..3591)
                     /gene="lon"
                     /locus_tag="VF874_17930"
     CDS             complement(1276..3591)
                     /gene="lon"
                     /locus_tag="VF874_17930"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX9177867.1"
                     /translation="MPEPFNVPVLFVADQVVLPGMVVPIALDDAARAAVEAARATDSG
                     KLLIAPRLDDRYASYGVIASILQVGRVAGGTAAVVRGERRAHIGAGASGPGTALWVEV
                     TEVDEGQPSDETKALAAEYKKLLLAMLQRREAWQIVDVVNQLTDPSALADTAGYASYL
                     SVEQKRQLLETPDVAERLRVLIDWIGEHLAEVEVSDKIAEDVRTGMEKQQKEFLLRQQ
                     MAAIRKELGEGEADGSDDYRARVEAAELPEKVREAALREVGKLERTSEQSPEGGWIRT
                     WLDTVLDMPWNVTTEDSTDLKAAREILDADHHGLEDVKDRIVEYLAVRARRASRGMQV
                     VGGRGSGAVMALAGPPGVGKTSLGESVARALGRKFVRVALGGVRDEAEIRGHRRTYVG
                     ALPGRIVRAISEAGSMNPVVLLDEIDKVGSDYRGDPSAALLEVLDPAQNHTFRDHYLD
                     LDLDLSDVVFLATANVVENIPSALLDRMELVQIDGYTEDDKVAIARDYLLPRQREKAA
                     LTDDEVTVTEAALRKIAADYTREPGVRQFERLLAKALRKATTKLADSADPITIDEPDL
                     VDYLGRPRFTPESAERTAVPGVATGLAVTGLGGDVLYIESGATDGEPGLQLTGQLGDV
                     MKESAQIALSYVRSHAAELGVDPKTLDRKIHVHVPAGAVPKDGPSAGVTMVTALVSMA
                     TGRQVRSDVGMTGEVTLNGRVLPIGGVKQKLLAAQRAGLSTVFIPARNEPDLDDVPAE
                     VLEALDVRPMTDVADIVAAALEPADAVTAAA"
     gene            complement(3620..3826)
                     /locus_tag="VF874_17935"
     CDS             complement(3620..3826)
                     /locus_tag="VF874_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177868.1"
                     /translation="MLVDSGGIHFVAWCPPSNMQDPTAFPTPGRHAKRVAPTIAHVRL
                     NKGTAVRYQGIRVTAREQTQADKG"
     gene            4064..4438
                     /locus_tag="VF874_17940"
                     /pseudo
     CDS             4064..4438
                     /locus_tag="VF874_17940"
                     /inference="COORDINATES: protein motif:HMM:NF023742.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          668 a         1555 c         1450 g          765 t
ORIGIN      
        1 cgtgtgcacg gcgttgggcc tgtccgaccg cggtgggccg ggcagtgccg aactgtcatt
       61 gatccgctcg gggcgcgcac cgggcagctt ccacgtcacg cgggcgctga cagcctgaca
      121 cgcgacacgc ccactgagac aacggtattg agacagcgac gccccccaaa cctgaccggg
      181 accggggggc atcactcatc acagagaggg acatggagat gtcattcgcc gtactggacc
      241 ctactgcact cgccccgtct gacgagcaga tcgcggaagc gatcctggcg gcgcttccgg
      301 ccgacggggc gccggtcctg tggacccgta tccgcacccg gctgcccggt tcgcactggt
      361 cgcgggaaac caccctgctg cgactgcaca cgtgcggcga agtgcgcctg cgcaagttcc
      421 agcgcgccct cttcgtgtcg ttgggtgatg acgtcagccg cgaaactgct tgccgggcac
      481 gggcgggcgg tcgcgtgctg caggcgtcgg tggtcgcgta agtccgaacg cccgttcggt
      541 tggctgcgtt ttggctacat ttctggctga ccgccgttaa tcggtgttga acacagttga
      601 ccccaacgcg gcaggtcgcc gggccggtcc gcacatcgtc caagatgcca gcgacgcgct
      661 aatagagtcc gtttctcttc gaggctgcgt tgcccgccca ggagttcgcg gacgcggtcg
      721 gcgaggtctt cgtcatacgc cacgcgccca ttccccctct tcggccgagc gtgcggctca
      781 gtccagatcg acccgaatgg tcagcaggtc gctgcccatc agccgcacta cggcgctgtt
      841 gagcgtgggc agttcaccca accgggcgat tgggtcgtcg tcgggcagca gatgcgcggt
      901 cccggtgcgc cattggccac cgacgcgcac gcgcactttg ggattcgcct tgatgttgtg
      961 cacatagtgc gagtgctcgc cgtgttcgga gacaaaccag aactggttgt ccagcatccg
     1021 cccgccgacc gcggtgcgcc tcggctgccc ggacttgcgt ccggtggtct cgatcatcgt
     1081 gatcggcagc tgccggccga tggggttgac gaggcggcgc tggacacctt ggacgacgcg
     1141 tctcttgagg gtctgcaaat cggtcaccca cctattcaac gccgtgcctg cattcagcgc
     1201 gagcgtgcat gtttgtaggt atccccttac gttggtgtcg gcctacaaac ccgcacgctc
     1261 gcggcaaaag gggggtcagg cggcggcggt gaccgcgtcg gctggctcca acgcagccgc
     1321 gacgatgtcg gcgacatcgg tcatcggcct cacgtccagc gcctcgagca cctcggccgg
     1381 gacgtcgtcc aggtccggct cgttgcgcgc cgggatgaac acggtcgaca gcccggcgcg
     1441 ctgcgcggcc agcagcttct gcttaacgcc gccgatcggc agcacccggc cgttcagcgt
     1501 gacctcgccg gtcatcccga cgtcggagcg gacctgccgc ccggtggcca tcgacaccag
     1561 cgcggtgacc atcgtgacac cggccgacgg gccgtccttc ggcaccgcac cggcgggcac
     1621 gtgcacgtgg atcttgcggt ccagcgtctt cgggtcaaca cccagctccg cagcgtggga
     1681 acggacgtag gacagcgcga tctgcgcaga ctccttcatc acgtcgccca gttgccctgt
     1741 cagctgcagg cccggttcgc cgtcggtcgc acccgactcg atgtagagga catcgccgcc
     1801 gaggcccgtc accgccagac ccgtcgccac gcccggcacc gcggtgcgct cggcggattc
     1861 cggcgtaaac cgtggccggc caaggtaatc caccaaatcc ggctcgtcga tcgtgatcgg
     1921 atcagcgctg tccgccagtt tcgtcgtcgc cttacgcagc gccttggcca gcagtcgctc
     1981 gaactgccga acccctggct cgcgggtgta gtccgcggcg atcttgcgca gcgccgcttc
     2041 ggtgaccgtc acctcgtcgt cggtcagcgc cgccttctcc cgctgccgcg gcagcaggta
     2101 gtcgcgggcg atcgccacct tgtcatcctc ggtgtagccg tcgatctgca ccagttccat
     2161 ccggtccagc agcgccgacg ggatgttctc gacgacgttg gcggtggcca agaaaaccac
     2221 gtcggacagg tcgagatcca gatcgaggta gtggtcgcgg aacgtgtggt tctgcgcggg
     2281 gtccaacacc tcgagcagcg cggccgacgg gtcgccgcgg tagtccgagc cgaccttgtc
     2341 gatctcgtca agcagcacca ccgggttcat cgatccggcc tcgctgatcg cgcgcacgat
     2401 ccggcccggc agcgcaccga cgtaggtgcg ccggtgaccg cggatctcgg cctcgtcgcg
     2461 cacaccaccc agcgccaccc ggacgaactt gcggcccaga gcccgggcca cgctctcgcc
     2521 cagcgacgtc ttgccgacac cgggcggacc ggccaaagcc atcacggcgc cggagccgcg
     2581 accgccgacc acctgcattc cgcgtgacgc gcgacgcgcc cgcacggcca ggtactcgac
     2641 gatgcggtcc ttgacgtcct cgagcccgtg gtggtcggcg tcgagaatct cccgcgcggc
     2701 cttcaggtcc gtggagtcct cggtcgtgac gttccacggc atgtccagca cggtgtccag
     2761 ccaggtgcgg atccagccgc cctcggggct ctgctcgctg gtgcgttcca gcttcccgac
     2821 ctcccgcagc gcggcctcac gcaccttctc cggcagttcg gcagcctcga cgcgggcccg
     2881 gtaatcgtcc gagccgtctg cctcgccttc gccgagctcc ttgcggatcg cggccatctg
     2941 ctggcgcagc agaaactcct tctgctgctt ctccattccg gtgcgaacgt cctcggcgat
     3001 cttgtcgctg acctcgacct cggcgagatg ttcaccgatc cagtcgatca gcacgcgcag
     3061 ccgttcggcc acatccgggg tctccagtag ctgacgcttc tgctcgacgc tcaagtacga
     3121 cgcgtagccg gcggtgtcgg ccagcgccga cgggtcggtc aactggttca cgacgtcgac
     3181 gatctgccac gcctcgcgcc gctgcagcat cgcgagcagc agcttcttgt actcggcggc
     3241 cagcgccttc gtctcatcgc tgggttggcc ctcgtcgacc tcggtcacct cgacccacag
     3301 ggcggtgccg ggaccgctcg cacccgcgcc gatgtgcgca cggcgctcgc cgcgcaccac
     3361 cgctgcggtg ccacccgcga cgcgtccgac ctgcagaatc gacgcgatca cgccgtacga
     3421 cgcgtagcgg tcgtccagcc gcggggcgat cagcagcttg ccggagtctg tcgcgcgggc
     3481 cgcctcgacg gcggcgcggg cggcgtcgtc gagcgcgatc ggcaccacca tcccgggcag
     3541 caccacttgg tcggcgacga acagcaccgg cacgttgaaa ggttcaggca ttcgtcctcc
     3601 aaagttaagc ctgatcggct caacctttgt ccgcctgagt ttgttcccga gccgtaacgc
     3661 gaatcccctg atagcggacg gcggtgccct tgttcagccg aacatgggcg atggtcggtg
     3721 cgacgcgttt ggcgtggcgg cccggcgtcg ggaaggcggt cgggtcctgc atgttcgacg
     3781 ggggacacca ggccacgaag tgaatgccgc cggaatcgac cagcacgtgc cttcttacgc
     3841 cgtctccttg gcaccgtcga acccgcacag ccgtccgccg cgcggatggt tcggcacgat
     3901 gaatcgggcg atgcgcgccc acatgtcgga caaatcggaa ggttggttcg ggtcatgaca
     3961 gccgatggtg accacgcggc cccttcgcgt cactggatgt ccgcgttttg tcggtgggtg
     4021 gtggcatgct caccgctgaa atgtccggca caccaccttc cgcttctgcc tcgatcccac
     4081 ggtcgagcag cacgaggtgc tggcccggca tgccggggcg gctcgattcg cgttcaacca
     4141 gtgcctgcgc atggtgaaag ccgcgctcac ccaacgcaaa accgatccag gtgtcgacgt
     4201 gccgtggtcg gggtttgatc tgatcaacag gtttaacgcg tggaagaaaa cccaggacgc
     4261 gggccgggtg ctggccgtgg acaccgatgg ggtcgccgag gttgtagtga ccggcctgcc
     4321 gtggcgggcc aggtgtgcca gcaggtgttc gaagaagccg ccgtggactg cgcccgcgct
     4381 ggccgcctgg tcggattcgc gctcgggtaa gcgcacaggc aaacgggtcg gcttcccc
//
LOCUS       DASWBF010000487         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1306260,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000487 DASWBF010000000
VERSION     DASWBF010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306260"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VF874_17945"
     CDS             <1..351
                     /locus_tag="VF874_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9177869.1"
                     /translation="HAALRINGFTVMLNDDFPETCGGKSMTPKSLGGTPVTIHLTVTD
                     VDDKFQQALDAGATVVAPLEDQFWGDRYGAVADPFGHHWSMGQPVREVSMDEIAAAMS
                     GSYFDLKKSGCGGP"
     gene            445..1806
                     /locus_tag="VF874_17950"
     CDS             445..1806
                     /locus_tag="VF874_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023869279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEX9177870.1"
                     /translation="MWDSILPAELLVLPVELGRVDGLLDDPVFFAPFAAYFDARIGRP
                     SIPMETYLRLMFLKFRYRLGYESLCREVADSISWQRFCRIPLGMRVPHPTTLMKITTR
                     CGDDAVAGLNEALLAKAATAKVLRTDKVRADTTVVEAAVAYPTDSGLLAKAIGAMART
                     VERIKAAGGATRTRARDRRRSAGRRARSIAAKLRLRGEQQRDQAQTAVRRITGELAGI
                     AEQAMREASSVIRNARRALRTATGQRKGRLHRAINHLGTIVGRTERVVAQARSRLSGV
                     MPESASRLVSLHDVDARPIRKGRLGKPVEFGYKAQIVDNADGVILDHNVEIGNPPDAP
                     QLAPAIARITGRAGRTPRAVTADRGYGEASVERDLHELGVRSVAIPRKSKPSAARREL
                     EHRRAFRDKVKWRTGSEGRINHIKRSYGWNRTELTGIGGARSWCGHGVFAHNLVKIGT
                     LTA"
     gene            1959..2222
                     /locus_tag="VF874_17955"
     CDS             1959..2222
                     /locus_tag="VF874_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177871.1"
                     /translation="MADDWQPGPAPYRIVLGTKRRIGGVEVQTSVAQFADGTIDHAGK
                     IELPTVHVQAYWGDNGLTAGQARQLARALIAAADELDGWADER"
BASE COUNT          430 a          773 c          769 g          349 t
ORIGIN      
        1 cacgccgcgt tacgcatcaa cggtttcacg gtgatgctca acgacgactt cccggaaacg
       61 tgcggtggca agtcgatgac gcccaagtcc ctgggtggaa ctccggtgac cattcacctg
      121 acggtcaccg acgtcgatga caagttccag caggcactgg acgcgggcgc caccgtggtg
      181 gctccgttgg aggaccagtt ctggggagac cgctacggcg cggtcgccga cccgttcggc
      241 caccactggt cgatgggaca gccggtgcgc gaagtcagca tggacgagat cgcggccgcg
      301 atgtcgggta gttacttcga cctgaaaaag tcggggtgtg gcggtccctg aactgggtgt
      361 gtttggacga gaggatggcc tcaacccctt gggctagaag ggatcgaggc catcgtgttc
      421 agaactgtaa acgaccagcc gacgttgtgg gattcgattc tgcccgccga gttgttggtg
      481 ttgccggtcg agttgggtcg ggtggatggg ctgctggatg atccggtgtt cttcgcgccg
      541 ttcgccgcat atttcgatgc gcggatcggt cggccatcga tcccgatgga gacctatctt
      601 cggttgatgt ttttgaagtt ccggtaccgg ctgggctatg agtcgttgtg ccgagaggtg
      661 gccgattcga tctcctggca gcggttttgc cggatcccgt tgggtatgcg ggtgccgcat
      721 ccgaccacgt tgatgaagat caccacccgg tgcggggatg atgcggtcgc cgggttgaac
      781 gaggcgctgc tggccaaggc ggccacggcc aaggtgctgc gcaccgacaa ggtccgcgcc
      841 gacaccaccg tggtggaggc tgctgtggcc tacccgaccg attcgggtct gttggccaag
      901 gcgatcggcg cgatggcccg caccgtggag cggatcaaag ccgccggtgg cgcaacccgt
      961 acacgggcgc gcgaccggcg ccgctcggcg ggtcggcggg cccgctcgat cgccgcgaag
     1021 ctgcgcctgc gcggtgagca gcaacgcgac caggcccaga ccgcggtgcg ccgtatcacc
     1081 ggggaactgg ccgggatcgc cgagcaggcg atgcgcgagg ccagctcggt gatccgcaac
     1141 gcccgacgcg cgttgcgcac cgcgaccggc cagcgcaaag gccggctgca ccgggcgatc
     1201 aaccacctgg gcaccatcgt gggacgcacc gaacgcgttg tcgcccaggc ccgtagccgc
     1261 ctgtccgggg tgatgcccga gtcggccagc cggctcgtca gcctgcacga tgtcgacgcc
     1321 cgcccgatcc gcaaaggacg cctgggcaaa ccggtcgaat tcggctacaa agcccagatc
     1381 gtcgacaacg ccgacggggt catcctcgac cacaacgtcg agatcgggaa cccgccagac
     1441 gccccccagc tggccccggc gatcgcacgg atcaccggac gcgccggacg cacaccgcgc
     1501 gcggtcaccg ccgaccgcgg ctacggcgaa gcatcggtgg aacgcgacct gcacgaactc
     1561 ggcgtacgca gcgtggcgat cccgcgcaaa agcaaaccca gcgccgcgcg ccgcgagctc
     1621 gaacaccgcc gagccttccg cgacaaggtc aaatggcgca ccggatccga aggacggatc
     1681 aaccacatca aacgaagcta cggctggaac cgcaccgaac tcaccggcat cggcggagcc
     1741 cgaagctggt gcggacacgg cgtcttcgct cacaacctcg tcaagatcgg gaccctcacc
     1801 gcatgaaacc cacacccgag cggcccgagc cgcacccgac cgccaccccc aatccatcac
     1861 catcgcggtg acaaagggtt tttcaggtcg aagtagttag gaaggacacc accaatgacc
     1921 accaccccac cccccgatgt ctgcctgccg gcgccgctgt ggccgatgac tggcagcccg
     1981 gacccgcgcc ataccggatc gtcttgggca ccaagcgccg tataggcgga gtcgaggtcc
     2041 aaacctcggt tgcacagttc gccgacggca ccatcgacca tgcgggcaag atagagctgc
     2101 cgacggtcca cgtgcaggcg tattggggcg acaatgggct caccgccggg caggcgcggc
     2161 agctggctcg cgcgctgatc gcggccgccg acgaactgga cgggtgggca gatgagcgat
     2221 gaacatcact ctccggctat tagggttgcc catcgcgcgt atcgaggtcg atgcgacccc
     2281 gctaatcgca gcgctagaca cccccgacag cggcggccac a
//
LOCUS       DASWBF010000488         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_59123, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000488 DASWBF010000000
VERSION     DASWBF010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59123"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VF874_17960"
     CDS             <1..1192
                     /locus_tag="VF874_17960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX9177872.1"
                     /translation="IDYADAAELAAKAAKAVQALRADSPPMWRMLRVQGVFVGRGAPG
                     KVAFLYTGQGSQYVNMLRDLRQREPIVAAMFDEADRIMTPLLGKPLTEYVFADSTDEA
                     AVQRVEQQLLQTEYTQPAVLATDLALTRMLDAYGVRPDMVMGHSLGEYGALVAAGALS
                     FDAALEAVSFRGREMAGLSTDDNGAMAAVSAPLAEIERIVAETDGYVVIANINSTSQA
                     VIGGVSDAVEAAVAAFKAAGMDARRIPVSHAFHTKIVAPASEPLKVALRRLDIRPPRL
                     PIVSNVTGDFYPADADVDTVLDLLGRQVASPVRFMDGLRTLYAAGARVFVEVGPKKAL
                     HGFVEDVLGDDVLALFTNHPKTGDVASFNQALCGLYAAGLGFPTLTAAAPDTAAVAAA
                     GRGR"
     gene            complement(1312..1826)
                     /locus_tag="VF874_17965"
                     /pseudo
     CDS             complement(1312..1826)
                     /locus_tag="VF874_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945970.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          352 a          661 c          717 g          368 t
ORIGIN      
        1 catcgactac gccgatgccg ccgagctggc ggccaaggcg gcaaaggcgg tgcaggcgct
       61 gcgggccgac agcccgccga tgtggcggat gctgcgcgtc cagggtgtct ttgtcggtcg
      121 cggcgcaccg gggaaggtcg cgtttctcta caccggccag gggtcgcagt acgtcaacat
      181 gctgcgcgac ctgcggcagc gcgaaccgat cgtggccgcg atgttcgacg aggccgaccg
      241 gatcatgaca ccgctgctgg gcaagccgct caccgagtac gtcttcgccg acagcaccga
      301 cgaagccgcc gtgcagcggg tggaacagca gctcttgcag accgagtaca cccagccggc
      361 ggtgctggcc acggatctgg cgctgacgcg gatgctcgac gcgtacggcg tgcggcccga
      421 catggtgatg ggccacagcc tgggtgagta cggcgccctg gtggccgcag gcgcgctgtc
      481 gttcgacgcc gccctcgagg cggtcagctt ccggggccgg gagatggcgg gcctgtcgac
      541 ggacgacaac ggcgccatgg cggcagtgtc cgcccctctg gccgagatcg agcggatcgt
      601 cgccgagacc gacggatacg tcgtcatcgc gaacatcaac agcaccagcc aggccgtgat
      661 cggcggtgtc agcgatgccg tggaggccgc ggtcgcggcg ttcaaggccg ccggcatgga
      721 cgctcgtcgg atcccggtca gccacgcgtt tcacaccaag atcgtggcgc cggccagcga
      781 accgctcaag gtcgcactgc gtcggctcga catccggccg ccgcgtctgc cgatcgtgtc
      841 gaacgtgacc ggtgactttt atccggcgga cgccgacgtg gacaccgtgc tcgatttgct
      901 cggccgccag gtggcgtcgc cggtccggtt catggacggg ctgcgcacgt tgtacgcggc
      961 gggtgcccgc gtcttcgtcg aggtcggccc aaagaaggcg ctgcacggtt ttgtcgagga
     1021 cgtcctcggc gacgatgtgc tcgccctgtt caccaaccac ccgaaaaccg gcgatgtcgc
     1081 ctcgttcaac caggcgttgt gcgggctcta tgccgccggg ctgggattcc ccacgcttac
     1141 cgccgcagcc ccggacactg cggcggtcgc cgccgccggt cgcggccgtt gacgcacctt
     1201 caatccaagc aggaggaccg cggcgaggca tcgtcaagtt atttgagaat acttgtggtg
     1261 cagtagatgt gagtggatta ggttgcggaa cttactgact gacggtgtct ttcatgccgc
     1321 tgcagcggct gtgatttcgt gccagaccgc gaaacggtcg cgcatctcgg ttcgccactg
     1381 ggatgcgctg atcagatgac gacggggccg aaagtgccct cggatgtttc cgaacgcaaa
     1441 caagaatcgc tgggcctgcc caggtgaggc gaagcgtttc atcacccgtt cgcggaccct
     1501 ggtcggctga tgggagttct cggcccggtt gagatacttc gagcggcgat gagtcaccga
     1561 cggcaccagc tcacggtggg ccagccgata gctggccagc ttgtcagtga cgatcacccg
     1621 cggcacgtat cgcagaccct tgagcaactt gcggaagaac cgcttggcag cagctgcgtt
     1681 gcgacgcgac tggaccagga tgtcgagcac gttgccgtgc tggtcgaccg cgcgccacag
     1741 gtaatggtgg tgccgttgat cgtcacgaag acctcgtcga ggtgccactt gtcgccgggg
     1801 cgggggcggc gccggcgcag ctggtttgtc cagaccggcc gccttgagtt cgcataaaag
     1861 tccgctgttg aaatgcgccg accgctcagc cgaggtggca acggtctggt acgcaacgaa
     1921 caccgccagc gccgcggcga gcgcaagcca ccaggttcgc gtccgggtca ccgtctatta
     1981 cttctagtgc gccacgaggc gccctgtcat attccggatt tagttaggag gaatttggac
     2041 gaacattcct gggtctgatg gcttacctga atccgaaagg cactgtcacg ttggtgag
//
LOCUS       DASWBF010000489        10772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_445549,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000489 DASWBF010000000
VERSION     DASWBF010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10772
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445549"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VF874_17970"
     CDS             <1..1343
                     /locus_tag="VF874_17970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9177873.1"
                     /translation="IIDVSGFEGTPTIEAFREILHGRLHKLDPMRFELVDIPFKFHHP
                     MWRENCEVDLEYHVRPLRLPSPGGRREFDEAIGEIASTPLDRSKPLWVMYFVEGLADG
                     NIGVINKVHHALADGVASGNLIAHAMDLKDIPDRDSYETDPPPSRSELMSAAFRDHLR
                     QIGRIPATVSYTVQGIGRFRRSSRKLSTLTRPFDPPPTFMNHALTPDRRFATATLALD
                     DVKETRKALGVTINDVVLTIAAGAVRTLLLKYHGHAEGPLVVSAPVSIDPDPDRIWGN
                     KFATLAIAVPTQIADPLERIHAVHTAAETAKESHQLLGPELFGRWAAYFPPAPVAAMS
                     QRTARTEKQTKLLNFVVSNVPGPRERGTVAGAVVREIYSVGPLISGSGLNITVWSYAD
                     QLSISVLTDGATVDDPHEVTDAMIDDFRALRTAAGLSENLTEVHDAMPLAAATS"
     gene            complement(1340..2563)
                     /locus_tag="VF874_17975"
     CDS             complement(1340..2563)
                     /locus_tag="VF874_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177874.1"
                     /translation="MTSRYAGLSREELVRLVPELLLIGQLIDRSGMAWCIKNFGHAEM
                     LQIAIEEWMGASPVYTKRMQKVLKFEGDDVVTIFKGLQLDIGAPPQFMDFRYTVHDRW
                     HGEFHLDFCGALMDVEPMGEDYVHGMCHDIEDPTFDATAAATNRKTQIRPIHRPPRVP
                     ADRHPHCAWTVTIDESYPPVQDHPALDIVRQTRAATIQLDEIDTSDAGASDYSGPLVS
                     DFDFATFSHSALVRIADEVCLQMHLLNLSFGIAVRKRAHDERQARDICTKQLIGIAGV
                     AAERIHRALDLPGGVGGALRVMHLHPLFNPAAYVDAEFTADAVHVHPSAAHDDGAWIS
                     LCSPAEARPLQAIVAAVDPHLDVGVTGTDTDWTAHVMRTDTAAGEFREVSVAKVSRGA
                     TFEFAPRRSLPLTVV"
     gene            complement(2553..2993)
                     /locus_tag="VF874_17980"
     CDS             complement(2553..2993)
                     /locus_tag="VF874_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9177875.1"
                     /translation="MDELRRKGLEKMNEVYAWEMPDMPGEFFSLTVDHLFGTIWTRPG
                     LSMRDRRMMLLAVLTALNQEELIEVQINAILQNEEMTREELREMAIFLTHYVGFPLGS
                     KLDGAVKRVVAKRKKAAESGAARDKKDNVNDAVKMHVGRTLHDK"
     gene            complement(2996..3889)
                     /locus_tag="VF874_17985"
     CDS             complement(2996..3889)
                     /locus_tag="VF874_17985"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9177876.1"
                     /translation="MSDVKLGYIGLGNMGAPMAMRLADWPGGLVVFDVRPEAMTPLVE
                     AGARPADGVADVAAADVISITVLNDEQVREVVGELARNAKPGTVVAIHSTISDDTAVE
                     LARELQPQGIHIVDAPVSGGGRAAKKGELATMVGATGEAFELIKEPFSRWASLVIHAG
                     EPGAGTRMKLARNMLHYISFAAACEASRLAEECGLNLQDLGKVVRHTDAITGGAGSIM
                     FRDDTKPIDPESFLYQPFLHARILGEKDLSLALALGEAHSVDLPLATAALERLAEGLG
                     VPHTVATSDALRASGSSTKEE"
     gene            complement(3899..4651)
                     /locus_tag="VF874_17990"
     CDS             complement(3899..4651)
                     /locus_tag="VF874_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177877.1"
                     /translation="MGQFDEKVAIVTGSGGGIGQAYAEALAREGAAVVVAEINAEAGE
                     RVAKQIVADGGTAIHVPVDVSDPESAKAMADRTLAEFGGIDYLVNNAAIYGGMKLDFL
                     ITVDWDYYKKFMSVNLDGALVCTRAVYKKMAKRGGGAIVNQSSTAAWLYSNFYGLAKV
                     GINGLTQQLSRELGGMNIRINAIAPGPIDTEATRTTTPQEMVADIVKGIPLSRMGTPD
                     DLVGMCLFLLSDQAKWITGQIFNVDGGQIIRS"
     gene            complement(4675..6147)
                     /locus_tag="VF874_17995"
     CDS             complement(4675..6147)
                     /locus_tag="VF874_17995"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX9177878.1"
                     /translation="MTASASTKVTELLIDGELVDASGGTFTTINPATEEPLGDAADAD
                     AADMDRAIAAARAAFDESDWSRDTALRVHCLRQLRDALNEEIETLREITVAEVGAPVA
                     LTHSAQLQGPVDDLAFATDTAEKYQWATDLGVASPLGIKTRRTLVREPFGVVGAITPW
                     NFPHQINFAKLGPALAAGNAVVLKPAPDTPWCAAEVGRIIAEKTDFPPGVVNIVTSSD
                     HRLGAQLAEDPRVDLVSFTGSTATGRAVMAAAAQTIKKVFLELGGKSAFIVLDDADLA
                     SASAVAAFTVATHAGQGCAITTRLVVPRDRYDEAVAAAAGTMASLKAGDPTNPGTICG
                     PLISARQRDRVEGYLDLAIKEGGSFACGGGRPADRPTGFYVEPTVITGLTNDARVARE
                     EIFGPVLVVLAHDGDADAVRIANDSPYGLSGTVFGTDTERVNWVVSRLRTGTINVNGG
                     VWYSGDAPFGGYKQSGIGREMGLAGFEEYLETKLVAESVS"
     gene            6324..6917
                     /locus_tag="VF874_18000"
     CDS             6324..6917
                     /locus_tag="VF874_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9177879.1"
                     /translation="MSSEAVTAVTNLGERRRNRRQEETIRKVLDAAVEMLRESSYSDL
                     TMRAVAARAGVAPATAYTYFSSKNALVAEVYLRLVRNVPLFTDVNQTTQQRVTAQLRE
                     LGLLVADEPELAAAATTALMGDEPEVRPVRDKIGLEVRRRIASALGPGAWPEVLTTLE
                     MIFYGALVQAGTKSLTYYQMADRLDSVIGLIMGGAKL"
     gene            6914..8128
                     /locus_tag="VF874_18005"
     CDS             6914..8128
                     /locus_tag="VF874_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9177880.1"
                     /translation="MTQTTSDAVVLDPYDYEFHEDPYPVYKRLRDEAPLYHNEELGFW
                     ALSRHRDVLQGFRNSTTLSNRDGVSLDPVSRGPHASKTMSFLAMDDPAHLRLRTLVSK
                     GFTPRRIRELEPRVTELAVKHLDAMLDSAHSGTVEYVAEFAGKLPMDVISELMGVPEA
                     DRDRIRALADGVMHREDGVTDVPASAIEASINLIVYYQEMVAERRKKLTDDLTSALLE
                     AEIDGDRLTDDEVLGFMFLMVIAGNETTTKLLANAAFWGHRDPDQLAPVYQDLSRVPL
                     WVEETLRYDTSSQILARTVSGELTLYDTTIPEGDVMLLLPGSAHRDERVFDDPDAYLI
                     GREIGSKLLSFGSGAHFCLGAHLARMEARVALTELFRRIRDYEVDEANAVRVHSSNVR
                     GFAHLPITVEVR"
     gene            8128..8949
                     /locus_tag="VF874_18010"
     CDS             8128..8949
                     /locus_tag="VF874_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9177881.1"
                     /translation="MPRFEPHPKRRPAIVAGASSGIGAATAVELAQRGFPVALGARRL
                     EKCQEVVDQIRADGGEAIALPLDVTDPESVKGFVHRATDELGDIEVLVAGAGDTFFGR
                     LHEISTDTFESQIQIHLIGANRLATAVLPGMVERQRGDVIFIGSDVALRQRPHMGAYG
                     AAKAALIVMATNLQMELEGTGVRASIVHPGPTKTGMGWSLPPESIGPMLEDWAKWGQA
                     RHDYFLRASDLARAVAFVAETPRGGFVVSMEVQPEAPLANAPKDRQQLKYPEDTR"
     gene            8946..10295
                     /locus_tag="VF874_18015"
     CDS             8946..10295
                     /locus_tag="VF874_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9177882.1"
                     /translation="MTTAAVPRVSGGEDEHDHLEEFRTDPIGLMQRVRDECGDVGWFQ
                     LADKHVILLSGAEPNEFFFRSADSDLDQAEAYPFMTPIFGKGVVFDADPERRKEMLHN
                     AALRGEQMKGHATTIENEVRRMIADRGDEGEIDLLDFFAELTIYTSSACLIGKKFREQ
                     LDSRFAHLYHELERGTDPLAYVDAYLPIESFRRRDEARKGLVALVQEIMNTRIAIPPA
                     DKSDRDMLDVLITVKDEQRNPRFSADEITGMFISMMFAGHHTSSGTASWTLIELLRHP
                     DVYAGVIKELDELYADGQSVSFHALRQIPRLENVLKETLRLHPPLIILMRVAKGKFEV
                     EGFPIHEGDLVAASPAISNRIPEDFPEPDDFVPERYDQPRQEDLINRWTWIPFGAGRH
                     RCVGAAFATMQIKAIFSVLLREYEFEMAQPSDSYRNDHSKMVVQLAQPAKVRYRRRV"
     gene            10307..10513
                     /locus_tag="VF874_18020"
     CDS             10307..10513
                     /locus_tag="VF874_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9177883.1"
                     /translation="MGFRIEVDLDLCQGHAMCELEAPDYFKAPKRGQVQILNAEPPED
                     ARAEIEQAVFACPTQALSIREKED"
     gene            10516..>10772
                     /locus_tag="VF874_18025"
     CDS             10516..>10772
                     /locus_tag="VF874_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9177884.1"
                     /translation="MPSFPREELEDWVERWLEANREAERKGDWMPLADFYDDEGTYGW
                     NIGPKEDVMCVGIDEIRDIALGLEMAGLENWHYPYQRVIID"
BASE COUNT         1881 a         3569 c         3464 g         1858 t
ORIGIN      
        1 gcatcatcga cgtcagcggc ttcgagggca cgcctaccat cgaggcgttc cgcgagattc
       61 tgcacggacg gctgcacaag ctcgacccga tgcgcttcga gctggtcgac atcccgttca
      121 agttccacca cccgatgtgg cgcgagaact gcgaggtcga cctcgaatat cacgtccggc
      181 cgctgcggct gcccagcccg ggcggtcggc gcgaatttga cgaggccatc ggcgagatcg
      241 cgagcacacc tctggatcgc agtaagccgc tgtgggtaat gtatttcgtc gagggcctcg
      301 ccgacggcaa catcggtgtc atcaacaagg tgcaccacgc gctggccgac ggcgtcgcgt
      361 caggcaacct gatcgcgcac gcgatggacc tgaaggacat ccccgatcgc gactcgtacg
      421 aaaccgaccc gcccccttca cgatccgagc tgatgagcgc cgcgttccgc gatcacctcc
      481 gccaaatcgg gcgaattccg gcgacggtga gctacaccgt ccaggggatc ggccggttcc
      541 gtcgcagttc gcgcaagcta tccacgctca cccgtccctt cgacccgccg ccgacgttca
      601 tgaaccacgc cctcacgccg gatcggcggt tcgccacggc gacgctcgcg ctcgacgacg
      661 tcaaggagac ccgcaaggcg ttgggtgtca cgatcaacga cgtggtcctc acgatcgcgg
      721 cgggggccgt gcgcacattg ctgttgaagt accacggtca cgccgaaggg ccgttggtgg
      781 tgtcggctcc ggtgagcatc gatcccgacc cggatcgcat ctggggcaac aagttcgcca
      841 ccctcgcgat cgcggtgccc acgcagatcg ccgacccgct cgaacgcatt catgcggtcc
      901 ataccgccgc cgagaccgcg aaagaaagcc accagctgct cggtcccgaa ctgttcgggc
      961 ggtgggcggc gtattttccg cctgcgcccg tggcggccat gtcgcagcgg acggcgcgca
     1021 ctgagaagca gaccaagttg ctgaacttcg tcgtgtccaa cgttcccggt ccgcgcgagc
     1081 gcggcacggt ggcgggagcc gttgtgcggg aaatctattc ggtcggcccg ttgatttccg
     1141 gtagcgggct gaacatcacc gtctggagtt acgccgacca gctcagcatc tcggtgctca
     1201 ccgacggcgc caccgtcgac gatccgcacg aagtgaccga cgcgatgatc gacgacttcc
     1261 gcgctttgcg cacggctgcg gggctgtccg agaacctgac cgaggtgcac gacgccatgc
     1321 cgctggcggc cgcgacgagc tagacgacgg tcagcggcag cgaacggcgc ggcgcaaact
     1381 cgaacgtggc accgcggctg accttcgcga ccgacacctc ccggaactcc ccggccgcgg
     1441 tgtccgtgcg catcacgtgc gccgtccagt ccgtgtcggt tccggtcacc ccgacgtcca
     1501 gatgcgggtc gacggccgcg acgatcgcct gcagcggccg cgcctcggcc ggcgagcaca
     1561 gagatatcca cgcgccgtcg tcgtgcgcgg cggacggatg cacgtgcacc gcatcggcgg
     1621 tgaactcggc atcgacatac gccgccgggt tgaacaacgg gtgcaggtgc atgacccgca
     1681 gggcgccccc gacaccgccg ggtagatcca gcgcgcggtg gatccgttcg gcggccaccc
     1741 cggcgattcc gatcagctgc ttggtgcaga tgtcgcgggc ctgccgttcg tcgtgcgcgc
     1801 gtttccgcac cgcgatcccg aaggacaggt tcagcaggtg catctgcagg cacacttcgt
     1861 cggcgatccg taccagtgcg gagtgcgaga acgtcgcgaa gtcgaagtcg gacaccagcg
     1921 gcccggagta gtcggaagcg ccggcgtcgc tggtgtcgat ctcgtcgagt tgtattgtgg
     1981 ccgcccgcgt ttgccggacg atgtccaacg cagggtggtc ctgcaccggc ggatatgact
     2041 cgtcgatggt cacggtccac gcgcagtgcg ggtgccgatc ggcgggtacc cgcggcgggc
     2101 ggtggatcgg gcgtatttgc gtcttccgat tggtggccgc cgccgtggcg tcgaatgtgg
     2161 ggtcctcgat gtcgtggcac atgccatgca cgtagtcctc gcccatcggt tcgacgtcca
     2221 tcaacgcacc acagaaatcg aggtgaaatt cgccgtgcca gcggtcgtgc acggtgtagc
     2281 ggaaatccat gaactgcggc ggcgcgccga tgtcgagctg cagacctttg aagatcgtga
     2341 caacgtcgtc gccctcaaac ttcaagacct tctgcatacg cttcgtatag accgggcttg
     2401 cgcccatcca ctcctcgatc gcgatctgca gcatctccgc atggccgaaa tttttgatgc
     2461 accaggccat tccggagcgg tcgatcagct gaccgatcag cagcagctct ggcaccagcc
     2521 tgacaagctc ctcgcggctc aggccggcgt acctacttgt catgcagcgt cctgcccacg
     2581 tgcatcttca ccgcgtcgtt gacgttgtcc ttcttgtccc tggcggctcc gctctcggca
     2641 gccttcttgc gcttggccac cacgcgtttg accgcgccgt ccagcttgga gcccaacggg
     2701 aagccaacgt agtgggtcag gaagatcgcc atctcgcgaa gttcctcgcg cgtcatctcc
     2761 tcgttctgca ggatcgcgtt gatctgcacc tcaatcagct cttcctggtt gagcgcggtg
     2821 agcaccgcga gcagcatcat ccgccggtca cgcatcgaaa gcccgggccg cgtccaaatg
     2881 gtgccgaaga ggtgatcgac tgtcagcgag aagaattcgc ccggcatgtc cggcatctcc
     2941 caggcgtaga cctcgttcat cttctcgagg cccttacggc gtagttcgtc catgatcact
     3001 cctctttcgt cgatgagccg cttgcgcgaa gagcatcaga cgtggccacc gtgtgcggca
     3061 caccgaggcc ttcggccagc cgctcgagag ccgcggttgc cagcggcagg tccaccgagt
     3121 gcgcctcgcc cagcgcaagc gcaaggctca agtccttctc gcccaatatg cgtgcgtgca
     3181 ggaaaggctg atacaggaag ctctcggggt caataggttt ggtgtcgtcg cggaacatga
     3241 tcgacccggc gccgccggtg atcgcgtcgg tgtgacgcac caccttgccc aagtcctgca
     3301 ggttcaggcc gcactcttcg gccaaccgtg acgcctcaca ggcggcggcg aacgagatgt
     3361 agtgaagcat gttgcgggcc agcttcattc gagtgccggc accgggttcc ccggcgtgga
     3421 tcacaagcga tgcccaccgg gagaacggct ccttgatgag ttcgaatgcc tcaccggtgg
     3481 cgccgaccat ggtggccagc tcgcctttct tggcggcccg tcccccaccg ctgaccggcg
     3541 catcgacgat gtggatgcct tgtggttgca gctctcgtgc cagttcgacg gcggtgtcgt
     3601 cgctgatcgt ggagtgaatc gcgaccacgg tgccgggctt ggcatttcgc gccagctcgc
     3661 cgaccacctc gcgaacctgc tcgtcgttca ggaccgtgat gctgataacg tcggccgccg
     3721 cgacgtccgc gacgccgtcg gccggccgcg cacccgcctc gaccagtgga gtcatcgcct
     3781 cggggcggac gtcgaacacg accagccccc cgggccagtc cgccaggcgc atggccatcg
     3841 gcgcacccat gttgccaaga ccgatgtagc ccagtttgac gtcactcata tgaactcctc
     3901 acgacctgat gatctggccg ccgtcgacgt tgaagatctg cccggtgatc cacttcgcct
     3961 gatccgacag caagaacagg cacatgccca ccaggtcgtc cggtgtgccc atgcggctca
     4021 gcggaatgcc cttcacgatg tcggcgacca tctcctgcgg ggtggtggtc cgggtggctt
     4081 ccgtgtcgat gggccccggt gcaatagcgt tgatccggat gttcatgccg ccaagttctc
     4141 gcgaaagttg ttgggtcaga ccgttgatgc cgaccttggc cagcccgtag aagttggagt
     4201 acagccacgc cgcggtcgac gactggttga cgatcgcgcc cccgccgcgc ttggccatct
     4261 tcttgtatac cgcgcgggtg cacacgagcg ccccgtcgag gttcacgctc atgaacttct
     4321 tgtagtagtc ccagtccacc gtgatcagga agtcgagctt catgccgccg tagatcgcgg
     4381 cgttgttgac caggtagtcg atgccgccga actcggccag cgtccggtcg gccatcgcct
     4441 tggccgattc cggatcggag acgtcgacgg gaacgtggat cgccgttccg ccgtcggcga
     4501 cgatctgctt ggccacccgc tcgccggcct cggcgttgat ctcggccacc accaccgccg
     4561 caccctcacg ggccagcgcc tcggcgtacg cctggccgat gccaccgccg gaaccggtga
     4621 caatcgccac cttctcgtcg aactgtccca cgctgcttgc cctttcgtcg tctgctagct
     4681 gacggactcg gccacgagct tggtttcgag gtactcctcg aaaccagcca ggcccatctc
     4741 acggccgatt cccgactgct tgtagccgcc gaacggcgcg tcaccggagt accaaacgcc
     4801 gccgttgacg ttgatcgtcc cggtgcgcag tcgcgacacc acccagttga cccgctcggt
     4861 atcagtgccg aacaccgtgc cggacaagcc atatggcgag tcgttggcga tgcgcaccgc
     4921 gtcggcgtcg ccgtcgtgcg ccagtaccac caggacaggg ccgaagatct cttcgcgggc
     4981 aacgcgggcg tcgttggtca ggccggtgat cacggtgggc tcgacgtaga agcccgtcgg
     5041 tcggtcggcg ggacgccctc cgccacaggc gaatgagccg ccctccttga tcgccagatc
     5101 cagatagccc tcgacgcggt cgcgctgcct tgccgaaatc agcggtccgc agatggtgcc
     5161 cggattagtc gggtcgccgg ccttgaggct cgccatcgtg ccggccgccg ccgcgactgc
     5221 ctcgtcgtaa cgatcgcgcg gaaccaccaa ccgggtggtg atcgcgcagc cctgccccgc
     5281 gtgcgtggcg acggtgaagg ccgcgacggc agacgcactg gccaggtcgg cgtcgtcgag
     5341 gacaatgaac gcggacttgc cgccgagttc gaggaagacc ttcttgatgg tctgcgccgc
     5401 cgcagccatc acggcacgcc cggtcgcggt ggatccggtg aacgacacca ggtcgacgcg
     5461 cgggtcctcg gccagctgag cgccgagacg atggtcgctc gaggtgacga tgttgaccac
     5521 accgggcgga aagtcggtct tctccgcgat gattcggccc acctcggccg cgcaccacgg
     5581 tgtgtcgggc gcaggcttca acacgacggc gttgccggcg gccagtgcgg gcccgagttt
     5641 ggcgaaattg atctgatgcg gaaagttcca tggcgtgatg gcgccgacga cgccgaacgg
     5701 ctcccgcacc agcgtgcgcc tggtcttgat gcccagcggc gacgcgacac cgagatcggt
     5761 tgcccactgg tacttttcgg ccgtgtcggt ggcgaacgcg agatcgtcga ccggtccttg
     5821 aagttgggcg ctgtgcgtga gggctaccgg cgcaccgacc tcggcgacgg tgatctcccg
     5881 cagcgtctcg atttcctcgt tgagcgcgtc gcgcagctgg cgcaagcagt gcacgcgcaa
     5941 tgcggtgtcg cgcgaccaat cggactcgtc gaaggcggct cgcgccgcag cgatcgcgcg
     6001 atccatgtcg gccgcgtcgg cgtcggcggc gtcgccgagc ggttcttcgg tggccggatt
     6061 gatcgtggtg aacgtgccac cgctcgcatc gacgagctcg ccgtcgatca acagctccgt
     6121 caccttcgtc gacgccgagg cggtcatcgc tccatctccc tggacaagtg tctgtttcga
     6181 cacttcggac agtagttgcc gacagacgca atagcaagcg ccgagtgact cgccctgccg
     6241 cgcaatcggt aataattcct gttcaaaggc gatatcgaac acgatggttg tgcatcacgg
     6301 ccgcggtgct atatcttgga cacatgtcca gcgaagcggt caccgccgtc acaaacctgg
     6361 gcgagcgccg gcgaaatcgt cgccaggagg aaacgatccg caaagtgctc gacgcggcgg
     6421 tcgagatgct ccgcgagtcg tcctattcgg acctgacgat gcgcgccgtc gcggcccggg
     6481 cgggcgtcgc gccggcgacc gcctacacgt acttctcgtc gaagaacgcg ttggtcgctg
     6541 aggtgtattt gcggctggtg cgcaatgttc cgttgttcac cgacgtcaac cagaccaccc
     6601 agcagcgcgt gaccgcgcag ctgcgtgagt tggggctgct ggtggccgat gagccggaac
     6661 tggcggccgc ggcgacgact gcgctgatgg gtgacgaacc cgaggtgcgc ccggtgcgcg
     6721 acaagatcgg actggaggtg cgccggcgga tcgcgtcggc actgggtccc ggcgcgtggc
     6781 cggaggtgct gaccacgttg gagatgatct tctacggcgc cctggtgcag gccggcacga
     6841 agtccctgac gtactaccag atggctgacc ggctcgacag cgtgatcgga ctgatcatgg
     6901 gaggtgccaa gttgtgaccc aaacgacctc agacgcagtg gttctcgacc cttatgacta
     6961 cgagttccac gaggacccgt acccggtcta caagaggctg cgcgacgagg cgccgctgta
     7021 ccacaatgag gagctcggat tctgggcgct gtcccgacat cgcgatgtgt tgcaaggctt
     7081 ccggaacagc accacgctgt cgaaccgtga cggcgtctcg ctggacccgg tctcgcgcgg
     7141 accgcacgcg tccaagacga tgtcgttcct ggcgatggac gatccggccc atctgcgcct
     7201 tcgcaccctg gtgtccaagg gcttcacgcc gcgccggatc cgcgaactgg agccacgggt
     7261 caccgaattg gccgtgaagc atctcgacgc gatgctggat tcggcacact ccggaacagt
     7321 cgaatacgtc gccgaattcg cgggcaagtt gccgatggac gtcatctccg aactgatggg
     7381 tgtcccggaa gccgaccggg accgcatccg cgctctcgcc gacggtgtca tgcaccgtga
     7441 ggacggcgtc accgacgtgc cggcgtcggc gatcgaggcg tccatcaacc tgatcgtcta
     7501 ctaccaggag atggtcgccg agcggcgcaa gaagctcacc gacgatctga cgtcagcgct
     7561 gctggaagcc gaaatcgacg gcgaccggct caccgacgac gaagtcctcg gtttcatgtt
     7621 cctgatggtg atcgccggta acgagacaac cacgaaactg cttgccaacg cggccttttg
     7681 gggacacagg gatcctgatc agctggcccc cgtctatcag gatctctcca gggtcccact
     7741 gtgggttgag gagacgctgc gctacgacac ctccagccag attctggcgc gcaccgtttc
     7801 gggtgagctc accctctacg acaccacgat ccccgagggt gacgtgatgc tgctgctgcc
     7861 cggctccgcc caccgcgacg agcgggtgtt cgacgacccc gacgcctacc tgatcggtcg
     7921 cgagatcggt tcgaagctcc tgagtttcgg cagcggcgcg cacttctgcc tgggcgcgca
     7981 cctggcacgc atggaggcgc gggtggcgct gaccgaactg ttcaggcgaa tccgcgacta
     8041 cgaggtcgac gaggccaacg cggtccgcgt ccattcgagc aatgtccgcg ggttcgccca
     8101 tttacccatc accgtggagg tccgctgatg ccccgcttcg agccgcaccc gaaacgacgg
     8161 ccggcgatcg tcgccggggc atcctcgggc atcggtgccg ccacggccgt cgagctggcg
     8221 cagaggggat ttcccgtcgc gctcggtgcg cgtcggctgg agaagtgcca ggaagttgtc
     8281 gaccagatcc gggccgacgg cggcgaagca atcgcattgc ccctcgacgt cactgatccc
     8341 gagtcggtga aggggttcgt gcaccgggcg acggacgagc tgggcgacat cgaggtgctg
     8401 gtcgccggcg ccggcgacac attcttcggt cggctgcatg agatcagcac cgacacgttc
     8461 gagtcgcaga tccaaatcca cctgatcggc gcgaaccgat tggccaccgc ggtgttgccg
     8521 ggcatggtcg aacgccagcg cggggacgtc atcttcatcg gctcggatgt cgcgctgcgt
     8581 cagcgcccgc acatgggcgc ctacggcgcc gcgaaggccg cgttgatcgt gatggccacc
     8641 aacctgcaga tggaactcga aggcaccggg gtgcgcgcat cgatcgtgca tccgggtccg
     8701 acgaaaaccg ggatgggctg gagcctgcca cccgaatcga tcggcccgat gctcgaggac
     8761 tgggcgaagt ggggacaagc ccgccacgac tacttcctgc gagcctccga tttggccagg
     8821 gcggtcgcgt tcgttgccga aaccccgcgc ggcggcttcg tcgtgtcgat ggaagtccaa
     8881 cccgaggcgc cacttgccaa cgcgcccaaa gatcgtcagc aactgaagta cccggaggac
     8941 accagatgac taccgcagcc gttccccgcg tgtcgggtgg cgaagacgag cacgaccacc
     9001 ttgaggagtt ccgcaccgac ccgatcgggc tgatgcagcg tgtccgcgac gagtgcggcg
     9061 acgtcggctg gttccagctg gccgacaagc acgtgatcct gctgtccggt gcggagccca
     9121 acgagttctt cttccgttcc gccgacagcg atttggacca agccgaggcg tacccgttca
     9181 tgacgccgat cttcggcaaa ggcgtggtgt tcgacgccga ccccgaacgg cgcaaggaga
     9241 tgctgcacaa cgccgcgcta cgcggcgagc agatgaaggg ccacgcgacc accatcgaaa
     9301 acgaggtgcg ccggatgatc gccgacaggg gcgacgaagg cgagatcgac ctgctggact
     9361 tcttcgccga gctgaccatc tacacgtcct cggcgtgctt gatcggcaag aagttccgcg
     9421 agcagctcga cagcaggttc gcgcacctgt accacgagct ggagcgcggc accgacccgc
     9481 tggcctacgt ggacgcgtac ctgccgatcg agagcttccg ccgccgcgac gaagcgcgaa
     9541 aaggcttggt ggcgttggtg caagagatca tgaacacccg gatcgccatc ccgccggccg
     9601 acaagagcga ccgcgacatg ctcgacgtgc tgatcactgt caaggacgag cagcgcaatc
     9661 cgcggttctc cgccgacgag atcaccggga tgttcatctc gatgatgttc gccggacacc
     9721 acaccagttc cggaaccgca tcgtggacgc tgatcgagct gttgcgccac ccggacgtgt
     9781 acgccggagt gatcaaggag ctcgacgagc tctatgcgga cggtcagtcg gtgagtttcc
     9841 atgcgctgcg ccaaattccg cggctggaga acgtgctgaa ggagacgctg cgcctgcacc
     9901 cgccactcat catcctgatg cgggtggcca agggcaagtt cgaagtcgaa ggctttccga
     9961 tccacgaggg cgacctcgtc gcggcgtcgc cggcgatctc caaccggatc ccggaggact
    10021 ttcccgagcc cgacgacttc gtccccgagc gttacgacca accgcgccaa gaggatctga
    10081 tcaaccggtg gacatggatt ccgttcggcg caggccggca ccgctgcgtc ggcgccgcgt
    10141 tcgccaccat gcagatcaag gctatcttct cggtgttgtt gcgcgagtac gagtttgaga
    10201 tggcgcagcc gtcggacagc taccgcaacg accactcgaa aatggtggtg cagctggccc
    10261 agccggccaa ggtgcgctac cgcaggcgcg tctgacgagg cgatcaatgg gattccggat
    10321 tgaagtcgac ctagacctct gccagggcca cgcgatgtgc gagttggagg ctccggacta
    10381 cttcaaggcg cccaaacgcg gtcaggtgca gatcctcaac gccgagccgc ccgaggacgc
    10441 ccgcgccgag atcgagcaag ccgtgttcgc gtgtcccacc caagccttgt ccattcgaga
    10501 gaaggaagac tgacaatgcc gtcattcccc cgtgaggaac tggaagattg ggtcgagcgg
    10561 tggctggagg ccaaccgcga agccgagcgc aaaggcgact ggatgccgct ggccgacttc
    10621 tacgacgacg aaggcaccta cggatggaac atcggcccga aggaagacgt gatgtgcgtc
    10681 ggcatcgacg agatccgcga catcgcgctc ggcctcgaga tggccggcct ggagaactgg
    10741 cactacccgt atcagcgcgt gatcatcgac ga
//
LOCUS       DASWBF010000490         8906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_636861,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000490 DASWBF010000000
VERSION     DASWBF010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636861"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VF874_18030"
     CDS             <1..242
                     /locus_tag="VF874_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEX9177885.1"
                     /translation="LYYPLVATLMIAPTKVYQGLPALTPHEAAKWMITAARRRPLRIT
                     PRMSLLSQAVDLVSPDLTAALMKRQRIQPNPVDPA"
     gene            256..642
                     /locus_tag="VF874_18035"
     CDS             256..642
                     /locus_tag="VF874_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9177886.1"
                     /translation="MEILASRMLIRPADYERSLAFYRDEIGLAIWREYGAGTVFFAGQ
                     SLIELAGHGHDDGTAGEFPGAIWLQVRDIYETERELSEREVPIAREARREPWGLHEMH
                     VVDPDGVTLIFVQVPPEHPLRRDTRG"
     gene            complement(639..941)
                     /locus_tag="VF874_18040"
     CDS             complement(639..941)
                     /locus_tag="VF874_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HEX9177887.1"
                     /translation="MVESFFENVLKWLHQGYPEGVPPKDYFPLLAVLKRSLTEEEVVA
                     AAQSILRQTDSDTPVTREQIQQAIHEITEQEPNPEEIKQVASRLASVGWPLAAATR"
     gene            complement(951..1220)
                     /locus_tag="VF874_18045"
     CDS             complement(951..1220)
                     /locus_tag="VF874_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177888.1"
                     /translation="MHYLESILRVLVIGLLLGAGLPAVFAVGLRAYSSGAGGTAHAAN
                     PALKFVGLALFALVAAVIVVAILWITRATIIHHFDVDLFPFLPKK"
     gene            complement(1241..2515)
                     /locus_tag="VF874_18050"
     CDS             complement(1241..2515)
                     /locus_tag="VF874_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX9177889.1"
                     /translation="MSAELVILVLLVATALVFDFTNGFHDTGNAMATSIATGALKPKV
                     AVLLAGVLNLVGAFLSVEVAATVTTQVLKIQDAKTGQLLPSINATTGLTIIFAGLIGG
                     ILWNLFTWLFGIPSSSSHALFGGLVGAGLAAIGTSGVNWSGITQKVLIPALAAPLIAG
                     IVAAIGTWLVYRITRHVVRKRREDGFRWGQIATASLVALSHGTNDAQKTMGVIALALI
                     TTGHLSGDVKHNGLPFWVVFSCALAIGLGTYIGGWRVIRTLGKGLVEIESPQGFAAEA
                     SSAAIILSSSAAGMALSTTHVATGSILGSGVGKPGAEVRWAVAGRMAVAWLITLPAAG
                     LVGALSFWLAHGVARFSSALAGDIVIFAILVAASFYMWWRAQKQKVDHTNVNADWDTT
                     TNSVVPAEVRETNGAVKQVKQPEQTQPKEPIA"
     gene            2743..3045
                     /locus_tag="VF874_18055"
     CDS             2743..3045
                     /locus_tag="VF874_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HEX9177890.1"
                     /translation="MNRFLSAIVSWLRAGYPEGMPPTDYLPVLALLTRRLTHDEVKLV
                     AAEAMKRGGFDHIDIGVAITQITDELPAPADVERVRQRLAAKGWPLDDPRDIEDAG"
     gene            3114..4190
                     /gene="menE"
                     /locus_tag="VF874_18060"
     CDS             3114..4190
                     /gene="menE"
                     /locus_tag="VF874_18060"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEX9177891.1"
                     /translation="MLSGRSDAVLPVPAWDSRERALLTTALRAGEAIDDDVALVASTS
                     GTTGAPKGAMLTAAALRASAWATHTRLGGPGQWLLALPAHHIAGMQVLIRSTLAGITP
                     VVLEVSSGFDVSSLPRAVSAMRSGRRYASLVATQLAKALSDPAATSALASLDAVLIGG
                     GPLPAGVAESASAAGVSVVRTYGMSETAGGCVYDGVPLDGVRVRVEDDRVVVGGATLA
                     KGYRNPFWPNPFDEPGWFRTDDAGSIDAGGVLNVHGRLDEGISTGGLTVLPGMVEAVI
                     ARHPAVGQCAVFGVPDDRLGQRVVVAVVPTSADQPPSLAQLREHVAHSLDATAAPREL
                     HLLDELPVRGIGKVDRRALAERFS"
     gene            4288..5637
                     /locus_tag="VF874_18065"
     CDS             4288..5637
                     /locus_tag="VF874_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177892.1"
                     /translation="MKVRSEAVAVPVAVVLVAAAFVVPHLRLGIVSPLIHVSPARFRQ
                     LADTAPIFGWWDTHVGWGTPFAVLIGVLAVVWGPTAAQRLLWRALPATAWATSAVWAF
                     SLAMVDGWKHGFAGRLTAEHEYLRQVPTVTDIPEALRSFARRIPDFQPDSWTTHVSGH
                     PPGALLTFVWLDRIGLGGGAWAGALCLLVGSSAAAAIVVTLRALDDEATARLAVPFVA
                     VAPTAIWIAVSADGYFAGVAAWGIALLALAVSRSVRVPVIAAAGAGLLLGWAIFLNYG
                     LGLIAFLAVAVLLTARRRDALTVLAAAIPAALAVVAVFAQAGFWWFDGYTLVQQRYWQ
                     GIANDRPFQYWVWANLASVVCAIGLGSVAGVSKVFDARAIRRRSGIHLLVLAALIAIL
                     CGDLSMLSKAETERIWLPFTIWLTAAPALLPRSAHRWWLAVNVAGALLLNHVIYTNW"
     gene            complement(5641..6762)
                     /locus_tag="VF874_18070"
     CDS             complement(5641..6762)
                     /locus_tag="VF874_18070"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HEX9177893.1"
                     /translation="MKMNAAVLWDVNEEWSVEEVELDGPKEGEVLVAYEATGLCHSDH
                     HVRAGDLPLALPIVGGHEGAGVVQEVGPGVRNLTVGDHVVGTFMPACGRCRWCSSGHQ
                     NLCDLGAELAAGAQLDGTFRIHARGRELGALAWLGTFAQYGTVPEASLVKIDDDIPLN
                     RACLVGCGVATGWGSAVNTAQVTPGDTVVVIGCGGIGSAAIQGARLAGAEKIVVVDIA
                     EGKRDKAFQFGATHFATSLPEATALVGDLTRGVMADSVLLTVGLIEASLVVDALTIVR
                     KGGAVVVTALASFTDASPALPMAVLAMVTIYQKRLLGSLYGESNARADIPRLLSLYRE
                     GKLLLDETVTAEYKLADINVAYDDMLAGRNIRGIVVHDH"
     gene            6868..8169
                     /locus_tag="VF874_18075"
     CDS             6868..8169
                     /locus_tag="VF874_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9177894.1"
                     /translation="MGFGFPSGLWRALDRHPPPGVDRLQRWRSPLRGPWLTSVLGLVL
                     LITLPIVIITGLLSYIAYGPQFGMAIPADVGWLKPPTFAWPTDPAWLYRLNQGLHVGL
                     GLVLIPVVLAKLWSVIPRLFVWPPSGSIAQVLERLSLLMLVGGILFEIATGVLNIQYD
                     YIFGFSFYTAHYFGAWVFIVGFLMHITLKIPRMITGLRSMSMRDVLRTKRADTVPEPA
                     DPDGLVATDPAPATVSRRGALALVGGGALFMAVITAGQTIGGWTRHSALLLPRGRDRG
                     KGPNDFQVNRTAVVAAITPEITGDSWRLTLNGGLAPVVLDRNALAAMPQHTARLPIAC
                     VEGWSTTETWSGVRLRDLARLAGVPEPHLARVASLERYGFNRAVLQRNQVMHPDALLA
                     LRVNGADLSLDHGYPARIIVPALPGVHNTKWVASIDFEADR"
     gene            8166..8690
                     /locus_tag="VF874_18080"
     CDS             8166..8690
                     /locus_tag="VF874_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177895.1"
                     /translation="MRRALARYREFYGSHPLHLLTMLAGFALLAYIIAVIKPQTLWNP
                     QVWWQSIAVWFAAAVIGHDLVLFPLYSFADRLLTLPAAKRTRQPAVPAVNYVRVPALG
                     SALTLLVFLPGIIEQGAPTYQAATGQTQEPFFARWLLLTAAMFAVSAVIYAIRLLRRR
                     PDWPPPAPPPDELQ"
     gene            complement(8635..>8906)
                     /locus_tag="VF874_18085"
     CDS             complement(8635..>8906)
                     /locus_tag="VF874_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9177896.1"
                     /translation="GLGGDPWRILVRASELLRRGGRCLAEFDCDATGVRRSWVRLEST
                     KAVGPWFRWASVGIDSAGRLAEEAGMAVTAIHRVGERVVASLAVS"
BASE COUNT         1376 a         2990 c         2990 g         1550 t
ORIGIN      
        1 cgctgtacta cccgctggtg gcgacactga tgatcgcccc gaccaaggta taccagggat
       61 tgcccgcgtt gaccccgcac gaggcggcta agtggatgat cactgccgcg cggcggcggc
      121 cgctgcgcat cacaccacgc atgtcgctgc tgagtcaggc agtcgacctc gtcagccccg
      181 acctcactgc cgcgctgatg aagcggcagc ggattcaacc caatccggtg gaccccgcgt
      241 gatagacacg cagtcatgga gatcctggcc agccggatgc tgatccgacc cgcggactac
      301 gagcgctctc tggcgttcta ccgcgacgag atcgggctgg cgatctggcg cgaatatggt
      361 gctggcacag tgtttttcgc gggccagtcg ctgatcgagc tggccggcca cggccacgac
      421 gacggcactg ccggcgagtt tcccggcgcg atctggctgc aggtccgcga catctacgag
      481 accgaacgcg agttgtcaga gcgtgaggtg ccgatcgccc gcgaggcccg tcgggagccg
      541 tgggggctgc acgagatgca cgtcgtcgac cccgacggcg tcacgctgat cttcgtgcag
      601 gttccgcccg agcatccgct ccgtcgcgac acccggggct agcgcgtggc ggcagccagt
      661 ggccatccga ccgacgccag gcgcgacgcg acctgcttga tctcctcggg attgggttcc
      721 tgctcggtga tctcgtggat cgcctgctgt atctgttcgc gtgtcaccgg agtgtcggag
      781 tcggtctgcc gcaggatcga ctgcgccgcc gccacgacct cctcctcggt cagcgagcgc
      841 ttcagcacgg ccagcaacgg gaagtagtcc ttcggcggaa cgccttccgg atagccctgg
      901 tgcaaccact tcagcacgtt ttcgaaaaag ctctccacca tcgagtcagc tcatttcttc
      961 ggcaggaacg ggaacaggtc gacgtcgaag tggtgaatga tcgtggccct ggtgatccac
     1021 aagatcgcga ccacgatcac cgcggcgacg agcgcgaaca gcgccaagcc gacgaacttc
     1081 aacgccgggt tggccgcgtg cgcggttccg ccggcgccac tcgagtacgc gcgcagcccg
     1141 acggcgaaca ctgccggcag gcccgcaccg aggagcagtc cgatgacgag cacccgcagg
     1201 atgctttcca agtagtgcat cagctagtcc ttcaggtcct tcaggcgatc ggttctttgg
     1261 gctgggtttg ctcgggttgc ttgacttgct tgacggcccc gttggtctcg cgaacctcgg
     1321 cgggcaccac cgagttggtg gtcgtgtccc agtcggcgtt gacgttggtg tggtcgacct
     1381 tctgcttctg cgcacgccac cacatgtaga acgacgcggc caccaggatc gcgaagatca
     1441 cgatgtcgcc tgccagcgcg gagctgaacc gggcgacgcc atgcgcgagc cagaacgaca
     1501 gcgccccgac cagacccgcg gccggcagtg tgatcagcca cgccaccgcc atgcgtccgg
     1561 ccaccgccca gcgcacctcg gcgccgggct tgccgacgcc gctgccgagg atcgagcccg
     1621 tcgcgacgtg ggtggtcgac agcgccatcc cggcggcgct ggaactcagg atgatcgcgg
     1681 cggacgacgc ctccgcggcg aagccctgcg gcgactcgat ctcgacgagg cctttgccca
     1741 gcgtgcggat gacccgccag ccaccgatgt aggtgccgag cccgatcgcc agcgcacagc
     1801 tgaagacgac ccagaacggc aacccgttgt gcttgacgtc tccgctgagg tgaccggtgg
     1861 tgatcagcgc caacgcaatc acgcccatgg tcttctgcgc gtcgttggtt ccgtgcgaca
     1921 gcgcgaccag ggacgccgtg gcgatctggc cccagcggaa tccgtcttca cggcgtttcc
     1981 gcaccacgtg gcgcgtgatc cggtacacca gccaagtgcc aatggccgca acgattcccg
     2041 cgatgagcgg ggcggcgagt gcggggatca gcaccttctg cgtgatgccg ctccagttca
     2101 ccccggacgt gccgatcgcg gccagcccgg cgccgacgag tccaccgaac agcgcgtgcg
     2161 acgagctgga cgggatgccg aacagccacg tgaacaggtt ccacaaaatt ccgccgatga
     2221 gcccggcgaa gatgatcgtg agcccggtcg tcgcgttgat cgacggcagc agctggccgg
     2281 tcttggcgtc ctggatcttg agcacctgcg tggtgacggt ggcggcgacc tccaccgaca
     2341 agaacgcgcc gacgaggttc agcacaccgg ccaagagaac cgccaccttg ggcttcaggg
     2401 cgcctgtggc gatcgaggtc gccatcgcgt tgccggtgtc gtggaacccg ttcgtgaagt
     2461 cgaagacgag tgccgtggcg acaagcagca ccaggatgac caactctgcg ctcatggcgc
     2521 tcattctgct ggcacagcgg gcgatgcacc caaaaaacgg caaccctgat ccacgtgtcg
     2581 atcaggcaat tcgtttgacc tgcgaaggag ttcaccaatt gttcatctgg tggcggcgga
     2641 gtgttggccg gcacgggcga tgcagcgtgg tgaatcaccg cgttcgctct acgatcgaga
     2701 ggtcgggaaa gccttggcgg caaccgtggg gaggctgacg cagtgaacag attcctcagc
     2761 gcgatcgtgt cctggcttcg cgcgggctac cccgagggca tgccgccgac cgactacctg
     2821 cccgtgcttg cgcttctgac gcggcggctc acccacgacg aggtgaaatt ggtcgccgcg
     2881 gaggcgatga agcggggcgg tttcgaccac atcgacatcg gcgtggcgat cacccagatc
     2941 accgacgagc tgccggcgcc cgcggatgtg gagcgcgtgc ggcaacgact ggccgccaag
     3001 ggctggccgc tcgacgatcc gcgcgacatc gaggacgccg gatagccacg ctgcgcgccg
     3061 tcgaggtccc cgccggcttg gacgttctgg aggtgctgcc ggtcgtcgag cgaatgctct
     3121 ccggccgcag cgatgcggtg ctgccggtgc cggcgtggga ctctcgcgaa agagctttgt
     3181 tgacaacggc tttgcgtgcc ggcgaggcga tcgacgacga cgtcgcgcta gtggcgtcga
     3241 cgtccggcac cacgggtgcc ccgaagggcg cgatgctgac ggctgcggcg ttgcgggcga
     3301 gcgcgtgggc aacccacacc cgcctcggcg gccccggcca atggctgctg gcgctgcccg
     3361 cgcaccacat cgccggcatg caagtgctga tccgcagcac gctcgcgggc attacacccg
     3421 tcgtgctcga agtgtcttcg ggtttcgacg tgtcttcgct gccccgcgcg gtcagcgcga
     3481 tgcgatccgg gcgccgatac gcgtccctgg tggcgacgca actggcgaag gcgttgtcgg
     3541 atccggcggc gacgagcgcg ctggcctcac tggacgcggt gctgatcggc ggtggacctc
     3601 tgccggccgg tgtggccgaa agtgcttcag cggcaggggt ttcagttgtg cggacatatg
     3661 gcatgagcga gacggccggc gggtgtgtgt acgacggggt gccgctggac ggggtgcggg
     3721 ttcgggtgga ggacgaccgc gtggtcgtgg gcggcgcgac gctggcgaag ggataccgca
     3781 acccattctg gcccaacccg ttcgacgaac cggggtggtt ccgcaccgac gacgcgggaa
     3841 gcatcgacgc cggcggcgtg ctgaatgtgc acggccggct ggacgagggc atcagcaccg
     3901 gcgggttgac ggtgttgccg gggatggtcg aggcggtgat agcgcggcac cccgcggtcg
     3961 ggcagtgcgc ggtgttcggc gtgcccgacg accggttggg ccaacgggtc gtcgtcgcag
     4021 tggtgccgac gtcggcggat cagccgccgt cgctggcgca gctgcgcgag cacgtcgctc
     4081 actcgctcga cgccaccgcg gcaccgcgcg agttgcacct cctcgacgaa ttgccggtac
     4141 gtggaatcgg aaaggtggac cggcgggcac tggccgagcg gttcagctga ccgctcgttg
     4201 tgcgcgaaac tgaagtttcg ctgacaaagt gcgagtagcg acgtgcgaaa cgccggtttc
     4261 gccgggggca gccataggct ggggccggtg aaggtccgca gcgaggcggt ggcggtgccc
     4321 gttgccgtcg tgctcgtcgc ggccgcgttc gtggttccac acctgcgcct gggcattgtc
     4381 tcgccgctga ttcatgtgtc gccggcacgc ttccgccaat tagcagacac cgcacccatt
     4441 ttcggctggt gggacaccca cgtcgggtgg ggcacgccgt tcgcggtgtt gatcggcgtg
     4501 ctggcggtgg tgtggggtcc gacggccgcg cagcggctgc tgtggcgagc gctgccggcg
     4561 accgcctggg ccacgtcggc ggtgtgggcg ttctcgctgg cgatggtcga cggctggaaa
     4621 cacggtttcg ccggcaggtt gaccgctgag cacgagtacc tgcggcaagt gccgacggtc
     4681 accgacatcc cggaggcgtt gcgcagcttc gcgcgccgca tccccgactt ccagcccgac
     4741 tcgtggacta cccatgtgtc gggccatccg cccggcgcgc tgctgacatt cgtctggctg
     4801 gaccgcatcg gcttgggcgg cggcgcgtgg gccggcgcgc tgtgcttgct cgtcgggtcg
     4861 agcgcggccg cggcgatcgt cgtcacgctt cgcgcgctcg acgacgaagc caccgcccga
     4921 ctcgcggtgc cgttcgtcgc ggtagcgccg accgcgatct ggattgcagt gtcggccgac
     4981 gggtacttcg ccggcgtcgc cgcgtggggc atcgcgttgc tcgcgctggc ggtcagccgc
     5041 agcgtgcggg tgcccgtgat tgccgccgcc ggggcgggac tgctgctcgg ctgggcgatc
     5101 ttcttgaact acgggctcgg gttgatcgcg tttctcgcgg tggcggtgct gctcactgcc
     5161 cggcgccgag atgcactgac cgtgcttgcc gccgcgatac ccgcggcgct ggccgtggtc
     5221 gccgtgttcg cacaggctgg attctggtgg ttcgacggct ataccctcgt gcagcaacga
     5281 tattggcagg gcatcgcgaa cgatcgcccg ttccagtact gggtctgggc caacctcgcg
     5341 tcggtggtct gcgcgatcgg cctcggcagc gtcgccgggg tcagcaaggt gttcgacgcc
     5401 cgcgcgattc gtcgccggtc cggcattcac ctgctggtgc tcgccgcgct gatcgcgatc
     5461 ctgtgcgggg acctgtcgat gctgagcaag gcggaaaccg aacgcatttg gttgccgttc
     5521 acgatctggc tcaccgccgc gccggccctg ctcccccggt cagcgcaccg ctggtggctt
     5581 gccgtaaacg tcgctggcgc gctgctgctc aaccacgtca tctacacgaa ctggtagtgg
     5641 tcagtggtcg tggaccacga tgccgcgaat gttgcggccc gcgagcatgt cgtcgtaggc
     5701 gacgttgatg tcggcgagtt tgtactcggc ggtcaccgtc tcgtcgagga gcagcttgcc
     5761 ctcgcgatac agactcagca gtcgcggaat gtctgcccgc gcattggatt ccccatacag
     5821 gctgcccagc agccgtttct ggtagatggt gaccatcgcc aacaccgcca tcggtagggc
     5881 cggcgaggcg tcggtgaagg acgctagcgc ggtgaccacc accgccccgc ccttgcggac
     5941 gatggtgagc gcgtcgacga ccagcgacgc ctcgatgagc ccgaccgtca gcaacacgga
     6001 gtcggccatc acgccgcgcg tcaaatctcc gaccagagcc gttgcctctg gcaacgaggt
     6061 ggcgaaatgt gtggctccga actgaaacgc tttgtcccgc ttgccctcgg cgatgtcgac
     6121 cacgacgatc ttctccgctc ccgccaagcg ggcaccttgg attgcagcgc tgccgatacc
     6181 tccgcagccg atcacgacga cggtgtcacc gggcgtgacc tgtgcggtgt tcaccgctga
     6241 accccagccg gtggcgacgc cacagccaac caagcaggcg cggttcagcg ggatgtcgtc
     6301 atcgatcttc accagcgagg cttcgggcac cgtgccgtac tgggcgaagg tgccgagcca
     6361 cgccaacgcg cccagctcgc gtcctctggc atggatccgg aaggtgccat caagttgggc
     6421 accggcggcg agttccgcac cgagatcaca cagattctga tgtccgctcg agcaccagcg
     6481 gcagcgtccg catgcgggca tgaaagttcc caccacatgg tcgcccaccg tcagattcct
     6541 aacccccggt ccgacctctt ggacgacgcc ggctccctcg tgcccaccca cgatgggaag
     6601 cgccaggggc aggtcacccg cgcgaacgtg gtgatcggag tggcacagcc cggtcgcctc
     6661 gtaggcgacg agcacctctc cttccttcgg accgtcgagt tcgacctcct cgacgctcca
     6721 ctcctcgttc acgtcccaca agaccgccgc attcatcttc atgcccggtg actttatggg
     6781 gcttgggttg gccggtgcct gattcggtaa gaattccgtc tccgcgaaag ggatgacgag
     6841 gcgcggtgag cggagtagat gggacgtatg ggcttcggct tcccgtccgg actgtggcgt
     6901 gcccttgacc ggcatccgcc gcccggcgtt gatcgcctgc agcgctggcg cagcccgctt
     6961 cgcggcccgt ggttgacctc ggtgctgggc ttggtgctgt tgatcacgct gcccatcgtg
     7021 atcatcaccg gactgctgtc ctacatcgcc tacggcccgc aattcgggat ggccatcccc
     7081 gccgacgtcg gctggctgaa gccgccgacg tttgcctggc cgaccgaccc cgcctggctg
     7141 taccggctca accagggcct gcatgtgggc cttggattgg tactcatccc cgtcgtgctg
     7201 gccaagttgt ggtcggtgat accgcgtctg ttcgtctggc caccgagcgg gtcgatcgcg
     7261 caagttctgg agcgcctgtc attgctcatg ctcgtcggcg gaatcctgtt cgagatcgcc
     7321 accggtgtgc tgaacatcca gtacgactac atcttcggct tcagcttcta caccgcccac
     7381 tatttcggcg cctgggtctt cattgtgggg ttcttgatgc acatcacgct gaaaatcccg
     7441 cggatgatca ccggcctgcg ctcgatgtcg atgcgagacg tgttgcgcac caagcgtgct
     7501 gacactgtgc cggagcctgc cgaccccgac ggcctggtcg ccaccgatcc ggcgcctgcg
     7561 acggtgagcc gacgcggtgc tttggccttg gtgggcggcg gtgcgctgtt catggccgtc
     7621 atcaccgccg ggcagacgat cggcgggtgg acacgccact cagcgctcct gctcccccgc
     7681 ggccgcgacc gcggaaaggg acccaacgac ttccaggtga accgcaccgc cgtcgtcgcc
     7741 gcgataaccc ccgagatcac cggggacagt tggcggctca ccctcaacgg tggtctcgcg
     7801 cccgtggtgc tggaccgtaa tgcgttggcc gccatgccgc aacacacagc acggctaccg
     7861 attgcgtgcg tcgagggctg gtcgaccacc gaaacatgga gcggtgtgag gctgcgggat
     7921 ctcgcccgcc tcgccggcgt gccggaaccg catttggcgc gggtcgcgtc gttggagcgc
     7981 tacgggttca atcgagccgt gctgcaacgc aatcaggtga tgcatccgga tgcccttctc
     8041 gcgctgcgcg tcaacggcgc cgacctctca ctggatcacg gataccccgc gcgcatcatc
     8101 gtccccgcgc tgccaggcgt ccacaacacc aagtgggtcg cctcgatcga cttcgaggcc
     8161 gaccgatgag gcgagcgctg gcccgttacc gcgagttcta cggatcacat ccgttgcact
     8221 tgctcacgat gctggccggt ttcgcgctgc tggcctacat catcgccgtc atcaagccgc
     8281 agacgttatg gaacccccag gtctggtggc agtcgatcgc cgtctggttc gcggccgcgg
     8341 tcatcggcca tgacctggtt ctgtttccgc tctactcgtt cgccgaccgc ctgctgacgc
     8401 ttcccgcagc gaagcgcacg cgtcaaccgg cggtcccggc agtgaattac gtccgcgtgc
     8461 cggcgctcgg gtctgcgctg acgctactgg tgttcctgcc gggcatcatc gagcaaggcg
     8521 cgccgacgta tcaggcggcg acgggccaga cgcaggaacc gtttttcgcc aggtggctgc
     8581 tgttgaccgc cgcgatgttc gccgtcagcg cggtgatcta cgcgatccgg ctcctcagga
     8641 gacggccaga ctggccacca cccgctcccc cacccgatga attgcagtga cagccattcc
     8701 ggcctcctcg gccagccgcc ccgcgctgtc gatacccacc gaagcccagc ggaaccacgg
     8761 cccgacggcc ttcgtcgatt ccagccgtac ccagctgcgg cgcacaccgg ttgcgtcaca
     8821 gtcgaattca gccagacacc ggccgccgcg ccgcagcagc tccgaggcgc gaacgaggat
     8881 acgccaagga tcgccgccga ggccga
//
LOCUS       DASWBF010000491         3302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_445571,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000491 DASWBF010000000
VERSION     DASWBF010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445571"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF874_18090"
     CDS             <1..198
                     /locus_tag="VF874_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177897.1"
                     /translation="GEMMADRLLIPVTGGIGVYEPKTGTFERMIPLDRGNVSGPIMTG
                     VIGATVVEQRGGTVVALGEAP"
     gene            complement(206..1000)
                     /locus_tag="VF874_18095"
     CDS             complement(206..1000)
                     /locus_tag="VF874_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9177898.1"
                     /translation="MTRVLAVANQKGGVAKTTTVASLGAAMEEKGRRVLLVDLDPQGC
                     LTFSLGHDPDKLPVSVHEVLLGEVEPSAALVDTSEGMTLLPANIDLAGVEAMLLMRAG
                     REYALKRALAKVKDEFDVILIDCPPSLGVLTLNGLTAADEVIVPLQCETLAHRGVGQF
                     LRTIADVQQITNSELKLLGALPTLYDSRTTHSRDVLVDVADRYDMPVLAPPIPRTVRF
                     AEATASGCSILASRKNKGSAAYRDLANSLLKHWKSGKPLASLTPEV"
     gene            1059..1667
                     /locus_tag="VF874_18100"
     CDS             1059..1667
                     /locus_tag="VF874_18100"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEX9177899.1"
                     /translation="MAPQTHRLLLLRHGETEWSRSGKHTSRTELDLTDEGREQAKSAG
                     ETLAQLRLDNPYVVSSPRKRALVTAELAGLKVDEVTPLISEWDYGDYEGTTTDEIRKA
                     VPDWLVWTHGCPGGETVAQVGERADRAIAMALEHMQSRDVLFVGHGHFSRAVLTRWIE
                     QPLYEGIRFAMVAATIAACGFEHNVRQISRLGTTGYPRPLYR"
     gene            1664..2755
                     /locus_tag="VF874_18105"
     CDS             1664..2755
                     /locus_tag="VF874_18105"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX9177900.1"
                     /translation="MNPSFVLSGPGGTLVAHGVAAAYCDIGSAQAALRSGRVPIIVGA
                     LPFDVSQPAALTAPQSHEFSDVLPFRPSGPLPAARVTATIPAAEEHGERIRRGLAALR
                     DPSSGLRKVVLARAVEVSADSPFDVLTILSRLADADPHAYCYFADLSPAGPGYEGRAL
                     IGASPELLVARHGDRVTCRPFAGSAPRSPDPQTDRAHGVALAESAKNQVEHALVVDEM
                     RKALDPLCVDLQIAPTPELSSTAALWHLSTPITARLRESSTTAIDLALALHPTPAVGG
                     VPTDAAAAMIAELEGDRGYYAGATGWCDQRGDGRWVVSIRCAELSADRLTARAYSGGG
                     IVADSDVDDEIAETTTKFRTMLSALRVTV"
     gene            2752..3234
                     /locus_tag="VF874_18110"
     CDS             2752..3234
                     /locus_tag="VF874_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9177901.1"
                     /translation="MTSVIRRARPGDEAEITAMIYELAHFEHAAEHCTITENQMRTAL
                     FGTQPTVHGHIAEVDGQIAASALWFLSFSTWEGAAGIYLEDLFVRPPFRRRGLARALL
                     RELARECVAAGYTRLTWAVLDWNVDAIALYDAIGGKPLSDWISYRVSGPVLSAFAESD
                     "
BASE COUNT          540 a         1111 c         1132 g          519 t
ORIGIN      
        1 ggcgagatga tggccgaccg gctgctgatc ccggtcaccg gcggcattgg ggtgtacgag
       61 ccgaagaccg gcaccttcga gcggatgatt cccctcgacc gcggcaacgt cagcgggccg
      121 atcatgactg gcgtcatcgg cgccaccgtc gtcgagcagc gcggcggcac ggtcgtcgcg
      181 ctcggcgaag cgccgtagcg cccgatcaga cctccggcgt gaggctggcc agcggcttgc
      241 ccgacttcca gtgcttgagc agcgagttcg ccagatcgcg atatgccgcc gagcccttgt
      301 tcttgcggct ggccagtatc gagcaccccg acgcggtggc ctcggcgaaa cgcaccgtac
      361 gcggtatcgg gggcgccagc acgggcatgt cgtagcggtc ggcgacgtcg accagcacgt
      421 cgcggctgtg ggtggtgcgc gagtcgtaca gcgtgggcaa cgcacccaac agcttgagct
      481 cggagttggt gatctgctgc acgtcggcga tggtccgcag aaactgtccg acgccgcggt
      541 gcgcgagggt ctcgcattgc agcggcacga tcacctcgtc ggcggcggtc aagccgttca
      601 gggtcagcac gccgagcgac ggcgggcagt cgatgagtat gacgtcgaac tcgtctttga
      661 ccttggccag cgcgcgtttg agcgcatact cgcgaccggc ccgcatcagc agcatcgcct
      721 cgacgccggc caggtcgatg ttggcgggca atagcgtcat gccctcgctg gtgtccacca
      781 gcgccgccga cggttccacc tcgccgagca gcacctcgtg caccgacacc ggcagcttgt
      841 cggggtcgtg gccgagggag aacgtcagac acccctgcgg gtcgaggtcg acgagcagca
      901 cccgccgacc cttctcctcc atcgccgccc ccaacgacgc gaccgtcgtg gtcttggcga
      961 ctccaccttt ctggttggcc actgccagta ctcgcgtcac gctgccatcc aagcacgcgc
     1021 accgatccag tcacgattgg tggggcagaa tcggtggcgt ggcgcctcag acgcaccgac
     1081 tgctgctact gcggcacggg gaaaccgagt ggtcgcgatc cggaaagcac accagccgca
     1141 ccgaactcga cctcaccgac gagggccgcg agcaagcgaa atccgccggt gaaacgctgg
     1201 cacagctgcg gttggacaat ccgtacgtcg tcagcagccc gcgcaaacgc gccctggtca
     1261 ccgcggagct ggccggtttg aaggtcgacg aggtgacgcc gctgatcagt gaatgggatt
     1321 acggcgacta cgagggcacc accaccgacg agatccgcaa ggcggtgccc gactggctgg
     1381 tgtggacgca cggctgcccc ggcggtgaga ccgttgcgca ggtcggcgag cgcgctgacc
     1441 gggccatcgc gatggcgctg gagcacatgc agtcgcggga cgtgctgttc gtcgggcacg
     1501 gccacttctc ccgcgcagtg ctgacccgct ggatcgagca gccgttgtac gagggaatcc
     1561 gcttcgcgat ggtggcggcg acgatcgcgg cgtgcggctt cgagcacaac gtccgccaga
     1621 tcagccggct cggcaccacc ggctacccgc ggccgctgta ccgatgaacc catcgttcgt
     1681 gctcagcggt ccgggcggga cgctggtggc ccacggtgtg gccgctgcct actgcgacat
     1741 cggctcggca caggccgcgc tgcggtctgg gcgagtgcca attatcgtgg gcgcgttgcc
     1801 ttttgacgtc agccagccgg ccgcgttgac ggctccgcag tctcacgaat tcagcgatgt
     1861 gttgccgttc cggccgtctg gaccgctgcc ggcggcgcgg gtgacggcga ccatcccggc
     1921 cgccgaggag cacggcgaac ggatccgacg cggcctggcg gcgttgcgtg acccgtcgag
     1981 cggactgcgc aaggtggtgc tcgcgcgcgc agtggaggtg tcggcggact cgccgttcga
     2041 cgtcctgacg atcttgagcc gcctggccga cgccgacccg cacgcgtact gctacttcgc
     2101 cgacctgtca ccggcaggcc ccggctacga aggacgtgca ttgatcggcg cgagccccga
     2161 actactggtc gcccgccacg gcgacagggt gacctgccgg ccgttcgccg ggtcagctcc
     2221 acgatcgcca gacccgcaga ccgaccgcgc ccacggcgtc gcgctggccg agtccgccaa
     2281 gaaccaggtc gaacacgcgc tggtggtcga cgagatgcgc aaagcgctgg acccgctgtg
     2341 cgtcgacttg cagatcgcgc cgacgccgga actcagcagc accgcagcgc tgtggcacct
     2401 gagcaccccg atcaccgccc gccttcgcga atcatcaacg acggcaatcg atctggcgtt
     2461 ggcactgcac ccgacacctg cggtcggcgg ggtaccgacc gatgccgccg ccgcgatgat
     2521 tgccgagttg gagggcgacc gcggctatta tgccggtgcc accgggtggt gcgaccagcg
     2581 cggtgacggc cgctgggtgg tgtccatccg ctgcgcggaa ttgtcggcgg accggttgac
     2641 cgcccgcgcc tactccggcg gcgggatcgt cgccgactcc gatgtggacg acgagatcgc
     2701 ggaaacgaca acgaagttca gaacgatgct gtcggcgctg cgggtgacgg tatgacgagc
     2761 gtgatccggc gcgcgcgccc cggcgacgag gcggagatca ccgcgatgat ctacgagctt
     2821 gcgcacttcg agcatgccgc cgaacattgc acaattaccg agaaccaaat gcgcacagca
     2881 cttttcggta cgcagccgac ggttcacggg cacatcgccg aagtcgacgg ccagatcgcg
     2941 gccagcgcgc tgtggttcct gagcttctcg acatgggaag gcgccgccgg aatctacttg
     3001 gaggacttgt tcgtccggcc accgtttcgc cgtcgcgggc tggcccgcgc cctgctgcgc
     3061 gagttggcgc gggaatgcgt cgccgccggc tacacccggc tgacgtgggc ggtgctggat
     3121 tggaacgtcg acgcgatcgc tctgtacgac gcgatcggcg gcaagccgtt gagcgattgg
     3181 atcagctacc gggtatccgg cccagtattg tcggctttcg ccgagtctga ttgatgggca
     3241 gcccatttca gcgctaacgg actgaacagc agtccaagtg tcagcaacgc gacgacgccg
     3301 ag
//
LOCUS       DASWBF010000492         6373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1306829,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000492 DASWBF010000000
VERSION     DASWBF010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306829"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF874_18115"
     CDS             <1..339
                     /locus_tag="VF874_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177902.1"
                     /translation="QPQLSGHGPQHGGPPPPPSSQSTQSFNLPQGAPQYGGYQSGPPS
                     GGFPQQAQQEGPPTPPTGFPTFAPPSSAPTTEAKAPPPHYDAPTQEVRAPEGLRAPDQ
                     PSSPSGPPPS"
     gene            346..1593
                     /locus_tag="VF874_18120"
     CDS             346..1593
                     /locus_tag="VF874_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HEX9177903.1"
                     /translation="MASKPLADRSGPAGPRQARDLLTVAFGPSLVALLVIAAVVLIQL
                     VAANSDMTGALGAIASMWLGVHQVPVIIGGRELSVMPLLPVFAMVWGTARVTARAAPA
                     AASWFVIRWIVASALGGPLLLTAIALAVIHDAASVMTDLQTPNALRAFGGVLAVHLIG
                     AVCGVGSRLGRRLLQALSLPVWLADSVRAAATGVLALLGLSGAITAAALVVHWGTMHE
                     LYSVTDSLFGQFSLTVLSVLYIPNVIVGTSAVAVGSSVHVGIATFSSFTVFGGDIPAL
                     PVLAAAPTPPLGPIWVLLLIAAAASAVALGQQCARRPLPALAAYAKLLVAAVVATAVM
                     ALLGYAAGGRLGNFGDVGVDQSTFAPAVFLWFAGIGGLTVAMAGGIARRPKPQPEPEP
                     AEPEPEPEADTDVIDPPPVPDRD"
     gene            1603..2232
                     /gene="purN"
                     /locus_tag="VF874_18125"
     CDS             1603..2232
                     /gene="purN"
                     /locus_tag="VF874_18125"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX9177904.1"
                     /translation="MQQPLRVPPTAPARLVVLASGTGSLLASLLATAVGDYPGRVVAV
                     GVDRDCPAVDIAKAADVPVYRVRLSDYPDRAAWDAAITDATADHRPDLIVSAGFMKIL
                     GPQFLSRYMGRIVNTHPALLPAFPGAHAVPESLDYGVKVTGCTVHLVDAGTDTGPILA
                     QEVVAVRDDDDEASLHERIKVVERRLLAEVIADVATRGVTWIGRKATIG"
     gene            2229..3791
                     /gene="purH"
                     /locus_tag="VF874_18130"
     CDS             2229..3791
                     /gene="purH"
                     /locus_tag="VF874_18130"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX9177905.1"
                     /translation="MTQYGRRPIRRALVSVYDKTGLLPLARSLHDAGVDIVSTGSTAK
                     TIADTGIPVTPVEFVTGFPEILDGRVKTLHPMVHAGLLADTRKPDHVAALQRLEIAPF
                     DLVVVNLYPFTETVDSGADADECVEQIDVGGPAMVRAAAKNHPSVAVVTDPLGYDGVL
                     AALRSGGFTLDERKKLAALAFRHTAEYDVAVAMWMSTALSSDDPSLPAWFAGTWRRSA
                     VLRYGENPHQRAALYVDDSSWPGLAQAEQLHGKQMSYNNFADADAAWRAAFDHEETCV
                     AIIKHANPCGIAISSVSVADAHRKAHECDPVSAFGGVIAANTTVSVEMAETVADIFTE
                     VIIAPAYEDGAVEVLARKKNIRVLVASEPVVGGLEFKQVSGGLLVQQRDALNAAGDNP
                     ANWRLAAGEPADPSVLADLKFAWRSCRAVKSNAIVVAKDGATVGVGMGQVNRVDAARL
                     AVERGGDRVRGAVAASDAFFPFPDGLETLVAAGVTAIVHPGGSVRDDVVTSAAAAAGV
                     TLYLTGARHFAH"
     gene            complement(4009..4761)
                     /locus_tag="VF874_18135"
     CDS             complement(4009..4761)
                     /locus_tag="VF874_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9177906.1"
                     /translation="MRLLNLKTALVALTITTIALVGAASPSSAAVTAAGPTVASVQPR
                     PGEVVGVAAPVTVTFTTPVANRAAAERAFTIDIPNSPSGQFSWLDNRTLEFKPDGFWP
                     SHSVIPLSVGGFKTDITTGAAVQGVADISAHTFTVSIDGQVARTMPASMGKPSRPTPI
                     GSFSVLSREQSVVMDSRTIGIPLSDPDGYKITAYYAERITGSGVYVHSAPWSVDSQGY
                     ANVSHGCINLSPDNAVWYYNTVHIGDPVIVQA"
     gene            4960..6342
                     /locus_tag="VF874_18140"
     CDS             4960..6342
                     /locus_tag="VF874_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003901050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX9177907.1"
                     /translation="MTQPNQLPRTLGELRASGHRERPVKQEIRENLLEALAEGRDVWT
                     GIFGFDDTVLPQLERALIAGHDVVLLGERGQGKTRLLRSLEGLLDEWTPVIAGAELGE
                     HPYRPITPGSIRRAEELGDDLPVAWKHRSERYTEKLATPDTSVADLVGDIDPIKVAEG
                     RSLGDPETIAYGLIPRAHRGIVAVNELPDLAERIQVSMLSVMEERDIQVRGYTLRLPL
                     DVVVVASANPEDYTNRGRIITPLKDRFGAEIRTHYPLELDAEVGVISQEAHLSAEVPE
                     YLLQVLARFARYLRESRSIDQRSGVSARFAIAAAETVAASARHRAALLGEADPVARVI
                     DLSNVIDVLRGKLEFESGEEGREQAVLEHLLRRATADTAQKLLGGIDVGPIVAAVEHG
                     SPVTTGERVSARDVLAALPEVPAVATIQRRLGATSDGQRAAAIELALEALYLAKRIDK
                     VSGDGETVYG"
BASE COUNT          968 a         2155 c         2232 g         1018 t
ORIGIN      
        1 cagccccagc tctcgggcca cggcccgcaa catggcggtc cgcccccgcc gccgtcgtcg
       61 cagtccactc agagcttcaa cctgccgcag ggcgccccgc agtacggcgg ctaccagagc
      121 ggcccgccca gcggcggctt cccgcagcag gctcagcagg agggtccgcc gaccccaccc
      181 accggcttcc ccaccttcgc accgccgagt tcagccccga caaccgaggc caaggcgcct
      241 ccgccacact acgacgcacc gacgcaggag gtgcgcgccc cggaaggact gcgcgctccg
      301 gaccagccgt cttcgccgtc cggcccgccg ccgtcataga aaggcgtggc ttctaagccg
      361 ttagcggacc gcagcgggcc ggcggggccg cgccaggcgc gggatctgct gacggttgcg
      421 ttcggtccgt cccttgttgc gctgctggtg atcgccgccg tggtgctgat ccagctggtt
      481 gccgccaaca gcgacatgac cggcgcgtta ggtgcgatcg cgagcatgtg gctcggcgtg
      541 caccaggtgc cggtgatcat cgggggccgc gagctgtcgg tgatgccgct tttgccggtg
      601 ttcgcgatgg tgtggggcac cgcgcgcgtc accgcacgcg cggcgccggc ggcggcctcc
      661 tggttcgtga tccgctggat cgtcgcatcg gcgctgggcg gcccgctgct gctgacggcg
      721 atcgcgctgg ccgtgattca cgacgcggcg tcggtgatga cagatctcca gacgccgaac
      781 gcgcttcggg cgttcggcgg cgtcctcgct gtgcacttga ttggcgctgt ctgcggtgtc
      841 gggtcgcggc tcgggcgccg gctgctgcag gcgctgtcgc tgccggtgtg gctggccgac
      901 tcggtgcgcg cggccgccac cggggtgctc gcgctgctgg gcctgtccgg tgccatcacc
      961 gctgcggcgc tggtcgtgca ctggggaacc atgcacgagc tgtactcggt caccgactcg
     1021 ttgttcggcc agttcagcct gacggtgctg tcggtgctct acatccccaa cgtgatcgtc
     1081 gggacttccg cggtcgcggt gggctccagc gtgcacgtcg gcatcgccac gttcagctcg
     1141 ttcacggtgt tcggcggcga catccccgcg ctgccggtgc tcgccgccgc gccgacgccg
     1201 ccgctgggcc cgatctgggt gctcttgctg atcgccgcgg ccgcgtccgc cgttgctttg
     1261 ggtcagcaat gcgcgcggcg gccgctgccg gcgctggccg cgtacgcgaa acttctggtc
     1321 gcagcggtgg ttgcgacggc ggtgatggcg ctgctcgggt acgccgccgg cggccggctc
     1381 ggcaacttcg gcgacgtcgg cgtcgaccag agcacgttcg cgcccgcggt gttcctgtgg
     1441 ttcgccggga tcggcgggct caccgtggcg atggccgggg ggattgcgcg acggccgaag
     1501 cctcagcccg aaccggagcc cgccgaaccc gaacctgaac ctgaggcgga cacggacgtc
     1561 atcgacccgc ccccggttcc cgaccgagat taggctgcga gcgtgcaaca gccgctccgt
     1621 gtgccgccca ccgcaccggc acggctcgtg gtgctggcct ccgggacggg atcgctgctg
     1681 gcgtcgctgc tggcgacggc ggtcggtgac tacccgggcc gcgtggtggc cgtcggtgtc
     1741 gatcgcgatt gccccgccgt ggacatcgcg aaggccgccg acgtgcccgt gtaccgcgtc
     1801 cggctgtcgg attaccccga ccgcgcggcg tgggacgctg cgatcaccga cgccacggct
     1861 gaccaccggc ccgacctgat cgtctccgcg ggattcatga aaatccttgg accgcagttc
     1921 ctttcacgct acatgggccg catcgtgaac acccacccgg cgctgttacc ggccttcccc
     1981 ggcgcgcacg ccgtgcccga gtcgctcgac tacggcgtca aggtaaccgg gtgcacagtg
     2041 catctggtcg acgccggcac ggacaccggg ccgatcctgg cacaggaggt cgtcgcagtg
     2101 cgtgacgacg acgacgaagc aagcctgcac gaacggatca aggtcgtcga aagacggctg
     2161 ctggcagagg tgatcgccga cgtggccacc cgcggcgtga cctggatcgg acgaaaggca
     2221 accatcggat gactcagtac gggcgcagac cgatccgtcg cgcgttggtc agcgtctacg
     2281 acaagaccgg tctgctccct ctggcccgca gcctgcacga cgccggcgtc gacatcgtct
     2341 cgaccggatc gaccgcgaaa accattgccg acaccggcat tccggtgacc ccggtggagt
     2401 tcgtcaccgg attccccgag atcctcgacg gccgggtcaa gacgttgcac ccgatggtgc
     2461 acgccggact gctcgccgac acccgcaagc ccgaccacgt cgcggcgttg cagcggctag
     2521 agattgcacc gttcgacctc gtcgtcgtca atctgtaccc gttcaccgaa accgtggact
     2581 ccggcgccga cgccgacgag tgcgtcgagc agatcgacgt cggcgggccg gcgatggtgc
     2641 gcgcggcggc caagaaccac cccagcgtcg cggtggtcac cgacccgctc ggctacgacg
     2701 gggtgctggc cgcgctgcgt tccggtgggt tcacactgga cgagcgaaag aagctggcgg
     2761 cgttggcatt tcggcacacc gcggaatacg acgtcgcggt ggcgatgtgg atgtcgaccg
     2821 cgttgtcgtc ggacgatccg tctttgcccg cctggttcgc cgggacatgg cggcggtcgg
     2881 cggtattgcg gtacggcgag aacccgcacc agcgggccgc actctacgtc gacgactcgt
     2941 cgtggccggg gctggcgcag gccgagcagc tgcacggaaa acagatgtcc tacaacaact
     3001 tcgccgacgc cgacgcggcg tggcgtgcgg cgttcgacca cgaggagacg tgcgtggcga
     3061 tcatcaagca cgcgaacccg tgcggcatcg cgatctcgtc ggtctcggtg gccgatgctc
     3121 accgcaaggc ccacgagtgt gatccggtga gcgcgttcgg cggggtgatc gcggccaaca
     3181 ccaccgtgag cgtcgagatg gccgagacgg tcgccgacat cttcaccgag gtgatcatcg
     3241 cgccggcgta cgaagacggc gcggtggagg tgctggcccg caagaagaac atccgggtgc
     3301 tggtggcgtc ggagcctgta gtggggggtc ttgagttcaa acaggtttcg ggtgggctgc
     3361 tggttcagca gcgggatgcg ctgaacgccg ccggcgacaa cccggcgaac tggcgcttgg
     3421 cggctggcga gccggcggac ccctcggtgc tggccgatct caaattcgcc tggcgcagtt
     3481 gccgcgcggt gaagtccaac gcgatcgtcg tcgccaagga cggtgcgacc gtcggggtgg
     3541 gcatgggcca ggtgaaccgg gtcgacgcgg cgcggctggc ggtcgagcga ggaggggaca
     3601 gggtgcgcgg ggctgtcgcg gcgtccgacg cgttcttccc gtttcccgac gggctggaga
     3661 cgttggtcgc ggccggcgtg acggcgatcg tgcacccggg cgggtcggtg cgcgacgacg
     3721 tcgtcacctc agcagcggcg gctgccgggg tgacgttgta tctcaccggg gcgaggcact
     3781 tcgcgcactg agaacgaatt tcgtcgcaag tttcaaaacc ccatacgccg agcccggcgg
     3841 cggccatact gttcagtgga ctcccagggc cgcgcctatg cctcgacgat cacgggatcg
     3901 ccaacatgga cagttcgacc acccggcggg cgttgtcagg gctgatgcag ccgtggctga
     3961 cgtcggcgtg gccctgggag tccgcccccg tcgccgaccg ccgacgccct acgcctgaac
     4021 gattacggga tcgccgatgt ggacggtgtt gtagtaccag acggcattgt cagggctgag
     4081 gttgatgcag ccgtggctga cgttggcgta gccctgcgag tccaccgacc atggcgccga
     4141 gtgcacgtag acgccgctgc cggtgatccg ctcggcgtag taagcggtga tcttgtagcc
     4201 atccgggtcg ctcagcggta tgccgatcgt gcgggaatcc atcacgacgc tttgctctct
     4261 ggacagcacg ctgaagctgc cgatgggggt tgggcggctg ggtttgccca tcgacgccgg
     4321 cattgtgcgc gcgacttgac cgtcgatgct cacggtgaac gtgtgcgcgg agatatcggc
     4381 cacaccctgc acggccgcgc cggtggtgat gtcggtcttg aacccaccga ccgacagcgg
     4441 gatcacgctg tgcgatggcc agaatccgtc gggtttgaat tccagggtcc tattgtctag
     4501 ccacgaaaac tgaccgctcg ggctgttcgg gatgtcgatc gtgaacgcgc gctccgcggc
     4561 agctcgattg gcgacgggcg tggtgaacgt cactgtgacc ggtgcggcca cacccaccac
     4621 ctcgccgggc ctcggctgca ccgaggcgac cgtcggaccg gctgccgtga ccgccgccga
     4681 actcgggctg gcggctccga ccagcgcgat tgtggtgatc gtcagcgcga caagcgcagt
     4741 cttcaggttc agcaaacgca tgcctagatc tacccgagcg cagggctgag cgaaacctct
     4801 tccggccgca ggactcatca actcacccac ctgtcccccc actcaccctg atttgatgac
     4861 ggacactaaa aacgatatcg acagaaagct ctccacgagc ggaaacagca cagatcaggc
     4921 cgttaagcca gccgcctgga caagcagtag cgtggagtgg tgacccaacc taaccaactt
     4981 ccgcgcaccc tcggtgagct gcgcgcgtcg ggccatcgcg aacgtccagt caagcaggaa
     5041 atccgcgaga accttctcga agcgctggcc gagggtcgcg acgtgtggac cggcatcttt
     5101 ggtttcgacg acaccgtgct gccccagctt gagcgcgcgc tgatcgccgg gcacgacgtc
     5161 gtgctgctgg gcgaacgcgg tcagggcaag acgcggctgc tgcggtcgct ggagggactt
     5221 ctcgacgagt ggacgccggt gatcgccggc gcggagttgg gcgagcatcc gtaccggccg
     5281 atcacccccg gctcgatccg ccgggccgag gagttgggcg acgacctgcc cgtcgcctgg
     5341 aagcaccgca gcgagcgcta caccgagaag ctcgccaccc cggacaccag cgtggccgat
     5401 ctggtcggtg acatcgaccc gatcaaggtc gccgaagggc gcagcctcgg cgacccggag
     5461 accatcgcct acgggctgat cccgcgcgcg caccgcggga tcgtcgccgt caacgaactg
     5521 cccgacctcg ccgagcggat ccaggtctcg atgctgagcg tgatggagga gcgcgacatt
     5581 caggttcgcg gctacacgtt gcggctgccg ctggacgtgg tcgtcgtcgc cagcgcgaac
     5641 cccgaggact atacgaaccg cggccgcatc atcacaccgc tgaaggaccg gttcggcgcc
     5701 gagatccgca cgcactaccc gctggaactc gacgccgagg tcggcgtgat cagccaggag
     5761 gcgcacctgt cggcggaggt ccccgagtac ctgctgcagg tcctcgcccg cttcgcccgc
     5821 tacctgcgcg aatcccggtc gattgaccag cgttccgggg tgtccgcgcg gttcgcgatc
     5881 gccgcggccg agaccgtcgc cgcatcggcg cggcaccgcg ccgctctgct cggcgaagcc
     5941 gacccggtgg cccgggtgat cgacctgagc aacgtgatcg acgtgctgcg cggcaagctg
     6001 gagttcgagt ccggcgaaga gggccgcgag caggcggtgc tcgagcatct gctgcgcagg
     6061 gccaccgccg acaccgcgca gaagttgttg ggcggcatcg acgttgggcc gattgtcgcc
     6121 gcggtcgagc acgggtcgcc ggtcaccacc ggcgagcgtg tgtcggccag ggacgtgctc
     6181 gcggcgctgc ccgaggtccc agcggtggcc acgattcagc ggcgtctggg cgcaacgtcg
     6241 gatggacaac gcgctgccgc aatcgaattg gctctggagg cgctgtatct ggccaagcgg
     6301 atcgacaagg tcagcggcga cggggaaacc gtgtatggct agacgcggcc gtgggcggtc
     6361 gtccctctac tcg
//
LOCUS       DASWBF010000493         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_59456, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000493 DASWBF010000000
VERSION     DASWBF010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59456"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..154
                     /locus_tag="VF874_18145"
                     /pseudo
     CDS             1..154
                     /locus_tag="VF874_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(147..608)
                     /locus_tag="VF874_18150"
     CDS             complement(147..608)
                     /locus_tag="VF874_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HEX9177908.1"
                     /translation="MRRDNSHHLVAAAHRRRADTLERARQALQELGETGQRRTVTQIA
                     AHAGVSRSWLYAQTELRDQLRRLTAISETAEPAPPARFERSPDASLRQRLTLAHQRIR
                     ELDDENRQLQNQIALLHGQLRANRIAGTHVADTVHDANAQLTPPNSRPRPR"
     gene            complement(595..2247)
                     /locus_tag="VF874_18155"
     CDS             complement(595..2247)
                     /locus_tag="VF874_18155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9177909.1"
                     /translation="MSCRVEANRTRTTPRSIKPLLDHLSATGAESLLDRPLDDWLAGL
                     PTAAALHTPRAFLGYAIECLLDLRDGTGWDCEYQRDVWRLRRLGVSGHDGAKLDFTAV
                     QPVWLRELAKRWCRWRMSCGVGLGQLRSDRLALVRLSQFMPGLASSSGPGALDRAALE
                     AYLARLAVEIPQPKTRSAEIGCVTGFLHAVRQHRWASLPAEAQLYPSDQPRRDETPAP
                     RAIAEFVMHQLESVANLDRITDPRVRLLVEVLIRTGLRIGDATRLALECLVRDPQGAV
                     YLRYRNHKMRRDAVVPIDDELTAMIEAQQARTRQRFPTTAVLLPRSSANPDGRLPIPT
                     ATFHLQLGQWLETCGVTDELGQPVHVTAHQFRHTAATRWINHDVPQEVVRRLLDHTSH
                     AMTAVYARLADTTIREQWERAQKINIRGEPVDTTIDGPLADAEWMKQNLARARMALPN
                     GYCGLPLQKSCPHSNACLTCPLFVTTAEFLPQHRKQLDDTRALIARAQTDGHTRLAEM
                     NRTVETNLLTIITTLEAGQHDCRCAAAGSETCCGKDLSDAPR"
     gene            complement(2957..3667)
                     /locus_tag="VF874_18160"
     CDS             complement(2957..3667)
                     /locus_tag="VF874_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEX9177910.1"
                     /translation="MSTAAASYKGHRYPVEIINHCVWLYFRFPLSFREVEELMLARGV
                     VVSYETIRRWCVKFGQAYANQLRRRRPRPGDKWHLDEVFVKINGTLCYLWRAVDQHGN
                     VLDVLVQSRRNAKAAKRFFRKLLKGLRYVPRVLVTDKLASYGVAHRELMPSVQHRQSR
                     YLNNRAENSHQPTRQRERAMKRFKSLRHAQRFLSAFSGISAHFRPRRHLLNATGWRSE
                     MADRFAVWHVVTELDNAA"
BASE COUNT          670 a         1328 c         1442 g          772 t
ORIGIN      
        1 ccgattgggg atgaacccgg agacgctgcg caagtgggtg cgccaggccg aggtcgacgc
       61 cggtgaggcg gcgggcgtgt cgagcgagga gaggcgcgag ctgcgggagc tacgccgcag
      121 gtgtcgcgag ctcgaatcga ccatcgttat cttgggcggg gtcggctgtt tggcggcgtg
      181 agctgggcgt ttgcgtcgtg gacggtgtcc gcgacgtggg tgccggcgat gcggttggcg
      241 cgcagctgtc cgtgcaggag tgcgatctgg ttctgcagtt gacggttttc gtcgtcgagt
      301 tcgcgaatgc gttggtgtgc gagggtgagg cgttgccgca gcgaggcgtc ggggctgcgc
      361 tcgaaccggg caggtggtgc cggctcggcc gtctccgata tggcggtcag tcggcggagt
      421 tggtcgcgca gttcggtctg ggcatacagc catgagcggg agacgccggc gtgggcggcg
      481 atctgggtga cggtgcgccg ctggccggtt tcgccgagtt cctgcagggc ttggcgggcg
      541 cgttcgaggg tgtcggcgcg acggcggtgg gccgcggcga cgaggtggtg gctgttatcg
      601 cggcgcatcg gataagtcct tcccgcagca tgtttcgctg cccgctgcgg cgcaccggca
      661 gtcgtgttgg ccggcttcga gggtggtgat gatggtgagc aggttggttt cgaccgtgcg
      721 gttcatctcg gccaggcggg tgtggccgtc ggtctgggcg cgggcgatca gcgcgcgggt
      781 gtcgtcgagc tgcttgcggt gttggggtag gaattcggcg gtcgtgacga acagcgggca
      841 ggtcaggcac gcgttggagt gcgggcacga tttctgtagc ggtaggccac aatagccgtt
      901 gggcagcgcc attctcgcgc gggcaaggtt ctgtttcatc cattcggcat cggcgagcgg
      961 tccgtcgatc gtggtgtcga cgggctcgcc acggatgttg atcttctggg cgcgttccca
     1021 ctgctctcgt atggtggtgt cggccagccg ggcatatacg gcggtcatgg cgtggctggt
     1081 gtggtcgagc agccgtcgga ccacctcctg cggcacgtca tggttgatcc accgagtggc
     1141 cgccgtgtgc cggaactggt gagcggtgac atggactggc tgaccgagtt catctgtgac
     1201 accacaagtt tcgagccact gcccgagctg gaggtggaac gtcgcggtgg gaatgggcag
     1261 ccgtccgtcg gggttggcgc tgctgcgcgg cagcaacacg gcggtggttg gaaaccgctg
     1321 ccgggtgcgc gcctgctgcg cctcgatcat ggcggtgagt tcgtcgtcga tgggcaccac
     1381 cgcgtcgcgg cgcatcttgt ggttgcggta gcgcaaatag accgcgccct gcgggtcgcg
     1441 gaccagacac tccagcgcca gccgagtggc gtcgccaatg cgcaggccgg tgcggatgag
     1501 aacctccacc agcagccgga cccgcgggtc ggtgatccgg tcgaggttgg cgacgctttc
     1561 cagttggtgc atgacgaact cggcaattgc ccgcggtgcc ggtgtctcgt cgcggcgggg
     1621 ctggtcgctg ggatacagct gcgcctcggc tggcagcgat gcccaccggt gctggcgcac
     1681 ggcatgcaga aacccggtca cacagccgat ctcggcgctg cgggtcttcg gttgtgggat
     1741 ctcaacggcc agtcgagcca ggtaggcctc cagcgccgcc cggtcgagcg cgcccggccc
     1801 cgacgagctc gctagtccgg gcatgaactg cgacaaacga acgagcgcga ggcgatcact
     1861 gcgtagctgc cccagcccga ctccgcacga catgcgccac cggcaccatc gtttggccag
     1921 ctctcgaagc cagactggtt gcaccgcagt gaaatccagt ttggccccgt catggccgga
     1981 aacaccgagc cgccgtagcc gccacacgtc acgctggtat tcgcagtccc agccggtccc
     2041 gtcgcgcaaa tcgagcaggc actcgatcgc gtaaccgagg aacgcgcgcg gcgtgtgcag
     2101 tgcggccgcg gtcggtagtc ctgccagcca gtcatcgagc ggacgatcca gcagcgactc
     2161 ggccccggtt gccgacaagt ggtccagcaa cggcttgatc gatcgcggtg tggtgcgggt
     2221 ccggttcgcc tcgacccgac acgacattgc aacgcgtagc cgatctccag ccgcagctgc
     2281 ggcgcaagcg gccgcaagtc gaagcgatcc tcgccgtagc tggcgcagta ggcgatgaac
     2341 tccgccgccg acgggcaacc acgcagccgc caccgccggt ggtgggaatg acaccaaccg
     2401 gcgtccagtt cggcccacaa catgcacccc gggatggcgc acaccgctgc cggcgtgccg
     2461 gccacgtccg gcttccaccg atcgaccgct gggcgtccgg ccttgtccca ggcgtgggaa
     2521 tgcgtgtagc acaaccgata ccgatgctgc ccgaagccgc aaccgggcac caggcacgag
     2581 tgcagcggcc ggtaccccat cacctctgga tcagcggtct tggcccaccc gtgccggtct
     2641 ggacgaccag cctttctcca ccggcccaga tgggccgggc acagcccggc gtagcggctc
     2701 gaacgcacac acccgggcac cgcgcacccg ggcgacccga tcaccgaccc gctgcccagt
     2761 gtgcgcagga tcatcaattt gtatttgtcg cggaagcccg tcggggccgg atgctgatcg
     2821 gcatcgatga cgcgcacctg ctcgacggac tgtccgcgca cgttgtgcac caggcactgt
     2881 cacgttagtt taaaaatgtg atggtgcaat tggtttcagt gctgggtgat ggaacagtgg
     2941 tgggattgtt cgtatttcag gcggcgttgt cgagttcggt gaccacgtgc cagaccgcga
     3001 agcggtcggc catctcgctt cgccagcctg ttgcgttgag cagatgtcgt cgtggtcgaa
     3061 agtgtgcgga gataccgctg aaagccgaca ggaaccgttg ggcatgccgc aatgacttga
     3121 agcgtttcat cgctcgttcg cgctgtcggg tgggctgatg agagttctcg gcgcgattgt
     3181 tcagataccg cgactggcgg tgttgcaccg acggcatcag ctcgcggtgg gctaccccgt
     3241 agctggccaa cttgtcggtc accagcacac gcggcacata ccgcaagccc ttgagcagct
     3301 tgcggaagaa ccgcttggcc gctttagcgt ttcgccggga ctgcaccaac acgtccaaga
     3361 cgttgccgtg ctggtcgacc gcccgccaca ggtagcacag cgtgccgttg atcttcacga
     3421 acacctcatc gaggtgccac ttgtcgccgg ggcgtgggcg ccggcggcgc agctggttag
     3481 cgtaggcctg cccgaacttg acgcaccagc gtcggatcgt ctcgtagctg accactacac
     3541 cacgtgccag catcaactct tcgacctcgc gaaagctcag cggaaaccgg aagtacagcc
     3601 acacgcagtg gttgatgatc tccaccggat accggtgacc cttatacgac gccgctgcgg
     3661 tgctcacccc accgatccaa ccagaccacc cgaggcggcc cggcccacca acgtgacagt
     3721 gccctcaagt gtcaaagtct cagcgacgaa gggtccctac gccgcttccg ggacttcgcc
     3781 cggaagcggt ggcccttcgg gtagcacaaa acggcggtct ggcgtgtagc tccgaccgcc
     3841 gcgccccgga tcccttgcgt caatctgtcg atggcttcgc cctttggtct cggctcgaga
     3901 cgaggtatct gcgttaccat caccgttgcg gcacaatatc tttccgccag acgcgagcgc
     3961 cctttggtcc acattcggac gaggatctgc gctggtgacc cgatgcccct caagcctgcc
     4021 actttgcttc cgtcatcgcc aacaatttgt cgatcgggac tcctcaagcg gctagtcaat
     4081 ctgcttgacc atgacgagac gttgttctac cgcacgatca tgtccgatcc ggcgcggttc
     4141 ctgccgatcg tgtacgaccc caccatcggc gaggcctgcc tcaaattcgg ccacatcttc
     4201 cgcggtccac ga
//
LOCUS       DASWBF010000494         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_114578,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000494 DASWBF010000000
VERSION     DASWBF010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114578"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VF874_18165"
     CDS             complement(<1..1207)
                     /locus_tag="VF874_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9177911.1"
                     /translation="MTEARSGGARRTEVEFRWAASSLTRSIATCAAVGLAAAVIGSRW
                     QLIAFVAPLIGVLSSIPWQRSAAKVYVHAKPGMVRCFEDEQVQTTAWVTTESADTAAQ
                     LEVTVPEGMDTELLDGGSAQSKTLAAQAARWGRYPLSATVDAVCAGGLLGARAAVEAA
                     EIFVFPVVPPQSTGIPRTELPDRLGTHLTRHIGPGVEYADVRAYLPGDQLRTVNWPVS
                     ARRGSLHVTERLTDRAADVVVLIDTSAQAAGPATEATERAVRGAVQVVQTALRNGDRA
                     GVVALGGRRPRWLGADIGRRQFYRVLDAVLGAGGEFEVTTGTLAPRAAVPAGAIIVAF
                     STLLDTEFALSLIDLRKRGHTVVAVDVLRGAPFETEQDPIITRMWALQRSAMYRDMGV
                     IGVNVVSWPE"
     gene            complement(1200..2168)
                     /locus_tag="VF874_18170"
     CDS             complement(1200..2168)
                     /locus_tag="VF874_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9177912.1"
                     /translation="MTMPATVTTAYSEAVLDEIERVVVGKRAALRLILTTVLAGGHVL
                     IEDLPGLGKTLIARSFASALGLNFTRVQFTPDLLPADLLGSTVYDMQSGRFDFRRGPI
                     FTNLLLADEINRTPPKTQAALLEAMAEEQVSIDGETHRLPSPFIVLATDNPIEYEGTY
                     PLPEAQLDRFAIRLELKYLSPPDETSMLRRRLERGSAQPTVSQVVDANDLLAMRESVE
                     QVTVHDDILHYVVSLAGATRNHPQVAVGTSPRAELDLVQLARARALLEGRDYVIPEDV
                     KTLAVPAIAHRISLRPEMWVRSVHGSDVVEQLLARLPVPRARGRRD"
     gene            complement(2165..2650)
                     /locus_tag="VF874_18175"
     CDS             complement(2165..2650)
                     /locus_tag="VF874_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177913.1"
                     /translation="MNKFVVLGFSLVIAVEVLALVMVDHRFVLALSGVAAAVVLLEGR
                     RLLTRSLATDQPESPSGGQAESMRRWISRTESVIRWADSSRADWDRHLRPRLAREFML
                     ATGQRMGRDPAALQATGRIVFGDELWQWVDPSNVARIGRRAPGPGRDALNEILERLER
                     V"
BASE COUNT          459 a          977 c          945 g          431 t
ORIGIN      
        1 cctcgggcca cgacacgacg ttgacgccga tcacgcccat gtcgcgatac atcgcggacc
       61 gctgcagggc ccacatccgc gtgatgatcg ggtcctgctc ggtctcgaac ggtgcgccgc
      121 ggaggacgtc gaccgcgacg acggtgtggc cccgcttgcg caaatcgatc agcgacagcg
      181 cgaactcggt gtcgagcagt gtcgagaacg cgacgatgat cgcccccgcc gggacggccg
      241 ctcggggcgc caaagtgccg gtggtgacct cgaattcgcc tccggcgccg agcacggcgt
      301 cgagcacccg gtagaactgg cgccgcccga tgtcagcgcc aagccagcgc gggcgtcgac
      361 cccccagcgc gacaaccccg gcccgatcgc cattgcgcaa cgcggtttgc accacctgca
      421 cggcgccgcg gacggctcgt tcggtggcct cggtcgccgg tccggcggcc tgcgcgctcg
      481 tgtcgatcaa caccaccacg tcggccgcgc ggtcggtcag ccgctcggtg acgtgcaggc
      541 tgccgcgccg ggcgctcacc ggccagttga ccgtgcgcaa ctgatcaccc ggcaggtagg
      601 cccgcacgtc ggcgtattcg acgccgggcc cgatgtgcct cgtgaggtgg gtgcccagcc
      661 gatcgggcaa ctccgtgcgc gggatgcccg tcgactgcgg gggcacaacg gggaacacga
      721 agatctcggc cgcctcaacc gccgcccgcg cgcccagcag accaccggcg cagacggcgt
      781 cgacagtggc cgacagcggg tatctgcccc accgcgcagc ttgcgcggca agcgttttcg
      841 actgggcaga accgccgtcg aggagttccg tgtccatccc ctcgggtacg gtgacctcga
      901 gttgcgcagc ggtgtcggcg gattcggttg tcacccaggc ggtcgtctgc acctgttcgt
      961 cctcgaagca acgcaccatt ccgggcttgg cgtgcacata gaccttcgcc gccgaccgtt
     1021 gccacggtat cgaggacagc acaccgatca gcggcgccac gaacgcgatc agctgccagc
     1081 gcgaaccgat caccgccgcg gcaagcccca ccgccgcgca cgtggcaatc gaacgggtca
     1141 aggacgaagc ggcccagcgg aattcgacct ccgtccggcg agctccgccg ctcctcgcct
     1201 cagtcacgtc gaccgcgcgc ccgcggaacc ggcagccgcg ccaacagctg ctcgacgacg
     1261 tctgagccgt gcacgctgcg cacccacatc tccggccgca ggctgatccg gtgcgcgatc
     1321 gccggaacgg cgagcgtctt gacgtcctcg gggatcacgt agtcgcggcc ctccagcagc
     1381 gcgcgggcgc gggcgagctg gaccaagtcg agctcggcgc gtgggctggt accgacggcg
     1441 acctgagggt ggtttcgggt ggcaccggcc agcgacacga cgtaatgcag gatgtcgtcg
     1501 tggacggtga cctgttcgac cgattcgcgc atcgcaagca ggtcgttggc gtccacgacc
     1561 tggcttactg tcggctgcgc cgaacccctt tcgagccgac gccgcagcat cgacgtctcg
     1621 tcgggcggag acaggtactt cagttcgagc ctgatcgcga atcggtcgag ctgcgcttcc
     1681 ggtagcgggt aggtgccttc gtactcgatc gggttgtcgg tggccagcac gatgaacggt
     1741 gaaggcagcc ggtgtgtttc gccgtcgatg ctgacctgtt cttctgccat ggcctccagt
     1801 agcgccgcct gggtcttggg cggggtgcgg ttgatctcgt cggcgagtaa caggttcgtg
     1861 aagatcggcc cgcgacggaa gtcgaaccgg cccgactgca tgtcatagac cgtcgagccg
     1921 agcaggtcgg cgggcagcaa gtcgggagtg aactgcacgc gagtgaagtt caaacccaac
     1981 gccgaggcga acgatcgcgc gatcagcgtc ttgccaaggc cgggaaggtc ttcgatcagt
     2041 acgtggccgc ccgcgagcac cgtggtgaga atcaatctga gtgcggcgcg cttacccacc
     2101 actacacgct cgatctcgtc gagcaccgcc tcggagtagg cggtagtaac cgttgcaggc
     2161 atcgtcatac ccgctccaat cgttcgagga tttcgttgag cgcgtcgcgc cccggccccg
     2221 gcgcccggcg gccgatccgc gcgacgttcg atgggtctac ccactgccac agttcgtcgc
     2281 cgaagacgat ccggccggtg gcctgcagcg ccgccgggtc cctgcccatc cgctggccgg
     2341 tggcgagcat gaattccctt gccagccgtg gccgtaagtg ccgatcccag tcggcgcgac
     2401 tggagtcggc ccagcggatc accgactcgg tgcgagagat ccagcgccgc atcgactcgg
     2461 cctggccgcc ggagggcgac tcgggctggt ccgtcgccag tgagcgggtc agtagccggc
     2521 ggccttccag caacacaact gcggccgcga cgcccgaaag cgcaagcaca aagcggtgat
     2581 ccaccatcac caacgccagc acctccaccg cgatcaccag gctgaagccg aggacgacga
     2641 acttgttcat gccacgctct tcagttccgc gaggaccagt tggagtgcgc acaccgcggt
     2701 ctcgcgatgc ccttcgttca tgacgtgcgg gctgaaccgg gcctcggcga acagctcgac
     2761 gaggtcggtg gcggtgccgg catggatggc atggtgttcg accgcgcggg cc
//
LOCUS       DASWBF010000495         8493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_785610,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000495 DASWBF010000000
VERSION     DASWBF010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785610"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VF874_18180"
     CDS             <1..191
                     /locus_tag="VF874_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HEX9177914.1"
                     /translation="RFLAACQDNLLQPWRRFAGGCRCNRPTVELMRACGFAVAADDVV
                     WRAMPPIVRPLMVGQATR"
     gene            894..4715
                     /locus_tag="VF874_18185"
     CDS             894..4715
                     /locus_tag="VF874_18185"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX9177915.1"
                     /translation="MAVIVTRAFYGQPEVSATRAVDEVVLLVCLLFVAGCMLRAARAA
                     TGRRRYGGIAMATALVGWAAGEVIVTVDELRTNFAQAPQPPSLADLVLIVYPVGACVS
                     LLCLSEGRGRDRWRLVLDAGIVATSLFAISWIFVVGELLRRGGSSAPVTLAHIVADVV
                     VITTAILVWSRAGADGRPSLSLVVGGIMSIGVADITIVYLTGVGGYHSGDIVDLARLA
                     GLGMLALAALSSVNERPADTSPMEVHSRARLWLPYLPLVLAGGVGLGYALGQSGYRPL
                     LAMAGVIVVLVLARQFVVLFENQRLLSHVAREAFHDSLTGLANRAHFLDRLERALVRR
                     RRQGEPVAVLCLDLDNFKTVNDELGHPAGDELLIRVAGRLRSCVCETCTVARLGGDEF
                     AVLIEDSVEELLATAHRVLDAFTSTIVIDGVSLTVRPSIGLTIATSEARCTVDELLRH
                     ADLAMYAAKRDGGGCMRSFVPELPFPYELPQLVDPVAGIANPLIRATPGSDPRLPAVK
                     QDLTTEAVGSVRWAPPAIWAALAALALGVAVFAASTFAHVAGSEGFFAEVLYPALNLF
                     AAGLIGLRAYRVRAERLAWILIAAGMASSGVGDVIYAAWVSDGQSPSAADPAYLASYP
                     LVYAGLLLLVRGCLQRVPTTIRLDALVCGLTAAAVAAALASGPIGAATTRATATVLVG
                     ILYPWGDLLLVALAAGMLPILGWRNEFRWGLLVAGFMLSVVADVVYLFETAGSSYRVG
                     TSLDAAWPAASLLVALASWLPSSSVSIRRGTGLSSYLAPVACTVAAIAVAVLANESRV
                     AVALAALSLIAVAARFAVTFRDLSALAETHRHAMTDDLTGLPNRRALATALTAAPVTE
                     SSKAAWSRSRRALLLLLLDIDDFQEINDLVGRHVGDELLCRIAERLAKSVPNENLLAR
                     AGDDEFAVLLADGADLSTAKAQAGALLEAFQAPFALDQITVQVDASIAIAVCPDHCEH
                     PQELLSRVETTLPHAKAAATKIAMYDSADELDHQNDSQLVDDLRSALSNGELTCYYQP
                     KIDARDGRVHSVEGLLRWHHPTRGLLLPEAFLPAAERAGLMRQLANTVIDIALTQIRS
                     WRREGITLTVAVNLSPTNLLDLDLVDTVERLLATHGLPAEALIVEITESTLATDSVRS
                     RSTVAALRRLGVRISLDDYGTGWSSLARLQDLSVDELKLDRVFVSRIAQDPRSMAIVR
                     STVALAHSLGADLVAEGVEDEATLGALRRYGCNITQGYVHSPPLPANEFEQWIAEYTP
                     TVNYPHAVATD"
     gene            4940..5907
                     /locus_tag="VF874_18190"
                     /pseudo
     CDS             4940..5907
                     /locus_tag="VF874_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(6171..6863)
                     /locus_tag="VF874_18195"
     CDS             complement(6171..6863)
                     /locus_tag="VF874_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9177916.1"
                     /translation="MLDTLPERLTLARQQHLSHVAFLELVLADEATRRDTSSAARRAR
                     GAGLDPGMRIDTWDDTAAVRYDRTLWNELTSLRFLDGPHGACLLGPVGVGKTHLATAL
                     GHIAVRRRVSVLMLRADAMFKRLKAARLDNSVEAEMRRLAQVQLLLIDDFALQPLDAT
                     ETADFYELVVARHHKASTVTTSNREPAEWLTMMSDPLLAQSAVDRLTSTAHELVIEGE
                     SYRRRQKPTRSG"
     gene            complement(6935..8449)
                     /locus_tag="VF874_18200"
     CDS             complement(6935..8449)
                     /locus_tag="VF874_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX9177917.1"
                     /translation="MDRKTVVRNVEAAVVAGLDRDGGEEQLTDELIGQVVAAVRPDRP
                     NGHGLAWESLEGQRDRIVRWVGQGLTVVKIADLLAREGVVVPHRTLHRFCVERTEYRG
                     RRDTVPVADGEPGVECQIDFARMGLIYDPQVGRRRVVHALIFTAVYSRHMFVWLTFSQ
                     TLEAIIAGCEAGWRFFGGVFKVLVPDNMKPIVAQADAINPRFTVGWLEYAQARGFATD
                     PARVASPQDKPRVERVVQYVRGNFFAGEEFVDLADAQARATTWCTGKAGLRIHGTTCA
                     QPAVVFAEHEAAVLLPAPDVTYQVPIYTEVTVHRDYHVQVGRALYSTPEHLLGQRIQA
                     RADDELVKLFRRGQLVKTHPRQAPGRRSTDPEDLPAEKTAYAMRDLQRLISAARGHGP
                     NVGIYAERLLDHQLPWTKMRQAYRLLGLAKRYGSEPVDTACGRALDLDVVSVAKIAAM
                     LEKATENTPTPAPRAARAAVPARFARDATEYRTNRTTPDWLRVIDGGAGTETQQ"
BASE COUNT         1292 a         2779 c         2847 g         1575 t
ORIGIN      
        1 cgcggttcct cgcggcgtgc caggacaacc tgctccaacc gtggcgccgc ttcgcaggcg
       61 gttgccgctg caaccgcccg accgtggagc tgatgcgcgc ctgcggcttc gctgtcgcag
      121 ccgacgacgt cgtgtggcgc gccatgccgc cgatcgtccg tccgctcatg gtggggcagg
      181 cgacgcggtg atctggacgg tcgtcgactt cgccggggcc tagaagaaac gaccggcttg
      241 tgccggtcgt cccatagcgg ggcggaatgt cgacgaaaca cctgacctcc tagcgggaga
      301 ccagcgcagc gagtcgacgc gccgccagca gcacgcgctc gtagtcgcgg ccacggggca
      361 gccacccaag agcggcctag tacctcggac cctgaggccg tatgtccaca cctgctgact
      421 tcaggatttc cagcacggtc gtacgtccta ggcccaaagt ggccgccacc cgccggctgc
      481 tcataccagg cccatagtgc tcaaccacgt cagctcgaag gtggcctgtg atcctgcgtc
      541 gttgcggcgc gggcggccat cgctcagcgt tcacatcagc cgcagaagca tcatactgtt
      601 tctctggcca caatctgttc gaccagaacc gttccagggc taccaaatgc gatgtcgcaa
      661 gacatctgaa tcggcccgaa ccactggttc gggccgattc ttttggctaa gtagggcgtt
      721 ttacttttgc gactgcgcta attcggtgca tgatcgcgca agatcacctt ttctgctgca
      781 tgggtgcgaa ccaaacaggg atggcgatga acctctcggc gcgaaatccg tggaggcgcc
      841 ttgagctgtc tgctcgtgcc ggttggctcg ttgtcggcgt gtatggcgtc gcgatggcgg
      901 tcatcgtgac gcgggccttc tacggccagc ctgaggtctc cgcaactcga gcggtcgatg
      961 aggtcgtctt gctggtctgt ctgttgttcg tggccggctg catgttgcgg gcggcccgcg
     1021 cggcgacggg gcgacggcga tatggcggga tcgcgatggc gactgccctg gtcggctggg
     1081 ccgcaggcga ggtcatcgtg accgtcgatg aactgcgaac gaacttcgcc caggcgcctc
     1141 aaccgccgtc tctcgccgat cttgtgctca tcgtgtaccc agtcggcgcc tgcgtgtcgt
     1201 tgctgtgtct gtcagagggc cgcgggcgag accgatggcg gcttgtgctg gacgccggca
     1261 tcgtcgcgac gtcgttgttc gccatctcgt ggattttcgt ggtcggcgaa ctgctgcgac
     1321 gtggcggcag ttcggccccg gtcacgctgg cccacatcgt tgccgacgtc gtcgtcataa
     1381 cgacggcgat cctggtttgg tcgcgtgcag gtgccgatgg ccggcccagt ttgagcctcg
     1441 tcgtcggcgg aatcatgtcg atcggcgtgg ccgacatcac catcgtctac ctgaccggag
     1501 tcggcgggta ccactccggt gacatcgttg acctggcacg gctggctggg ctggggatgc
     1561 tggccttggc tgcgctgtcg agcgtcaacg agcggcccgc cgatacctcg ccgatggagg
     1621 ttcattcccg cgcccggctg tggttgccgt acctgccgct ggtgctggcc ggcggtgtgg
     1681 gcctgggtta tgcactaggc caatccgggt acaggccgct gctcgccatg gcgggagtca
     1741 ttgtcgtttt agtgctcgcg cgccagttcg tcgtgctctt cgagaaccaa aggctgcttt
     1801 ctcacgtggc gcgggaggct tttcacgaca gcctgaccgg cctggccaat cgggcacatt
     1861 tcctcgaccg gctcgagcgc gccctggtac ggcgacgtcg ccagggcgag ccggtcgcag
     1921 tgctgtgcct cgacttggac aacttcaaga cggtcaacga cgaattgggt catcccgccg
     1981 gcgatgagtt gctgatccgg gtcgctggcc gcctcaggag ttgtgtctgt gaaacatgca
     2041 ctgtcgcacg cctcggcggt gacgaattcg cggttctcat cgaggactcg gtggaggaat
     2101 tgctggcgac cgcgcatcgg gtgctcgacg cattcacatc gacgatagtg atcgacgggg
     2161 tctcgctgac cgtgcgcccg agcatagggt tgaccatcgc cacgtcagag gcgaggtgca
     2221 cggtcgacga actcctaagg cacgccgacc tggccatgta cgccgccaaa cgggacggcg
     2281 gcgggtgcat gcgcagcttc gtgcccgagc tgccctttcc ttacgagctg ccccagctcg
     2341 tcgaccccgt agccggtatc gctaacccgt tgattcgggc gacacctggc agcgatccga
     2401 gattgcctgc ggtgaaacag gatctcacga cagaagctgt tggaagtgtt cgatgggctc
     2461 cgcctgccat ctgggctgcg cttgctgcgt tggcgctcgg ggtcgccgtc ttcgcggcgt
     2521 cgaccttcgc tcacgtcgcc ggcagcgagg gtttcttcgc cgaggtcttg tatccagcgc
     2581 tgaacctgtt cgcggcaggg ctcattggcc tgcgcgcata ccgggtcagg gccgaacggt
     2641 tggcctggat cctcatcgcg gccggcatgg cgagttcggg cgtcggtgac gtcatctacg
     2701 ccgcatgggt ttcggacggc cagtcgccgt cggcggcgga cccggcatac ctggcctctt
     2761 acccgctggt ctatgccggc ctgctgctgc ttgtgagggg ctgcctgcag cgcgtgccta
     2821 ccacgatccg tctcgacgcc ttggtgtgcg ggttgactgc ggcggctgtc gctgcggcgc
     2881 tcgccagtgg gccgatcggg gcggcgacca cccgcgccac cgcgaccgtg ctggtgggga
     2941 ttctttaccc gtggggcgat ctgctgctcg tagccctcgc cgccgggatg ttgccaatcc
     3001 ttgggtggcg caacgagttt cgctggggcc tacttgtcgc gggcttcatg ctgtctgtcg
     3061 tcgctgacgt cgtgtaccta tttgagaccg ctggcagctc gtatcgggtg ggcacctcgc
     3121 tggatgcggc ctggcccgcc gcgtcactat tggtggcgtt ggcgagttgg ctgccttcgt
     3181 cgtcggtgtc aatccggcgc ggtaccggac tcagctccta cctcgcgccg gtggcttgca
     3241 ctgtcgccgc gatcgcagtg gccgtcttag ccaacgaatc tcgggtcgcc gtggcgctcg
     3301 ccgcgttgag cctgatcgcc gttgccgcgc gattcgccgt gacgttccga gacctcagtg
     3361 cgttggccga aactcacagg cacgccatga ccgacgactt gaccggttta cccaaccgcc
     3421 gggcgctggc gacggcgctg accgctgcgc cggtcaccga atcatcaaag gcagcctggt
     3481 cacggtcacg acgcgcgctg ctgctgctgc tgctggacat cgatgacttc caagagatca
     3541 acgacctcgt cggtcgccac gtcggcgatg aactcttgtg ccgcatcgcg gagcgactgg
     3601 cgaaatcggt gccgaacgag aatttgctgg cgcgggccgg agacgacgag ttcgcggttc
     3661 tgctggcgga cggtgcggat ctcagtaccg cgaaagccca agccggagcc ctgctcgagg
     3721 cgtttcaggc tccgttcgcg ctcgatcaga tcaccgtcca ggtcgatgcc agcatcgcca
     3781 ttgcggtgtg tccggaccat tgcgagcatc cgcaggaact gctgagccgc gtcgaaacga
     3841 cgctacccca cgccaaggct gcagcgacca agatcgccat gtacgactct gcggacgagc
     3901 tggatcacca gaacgacagc cagctggttg acgacctgcg cagcgcgctt tcgaacggtg
     3961 agctgacttg ttactaccag cccaagatcg acgctcgcga cggacgcgtc cacagcgtcg
     4021 aggggttatt gcgctggcac catcccaccc gggggttgct gcttcccgag gcgtttcttc
     4081 ccgccgcgga gcgcgccggg ctgatgcgcc agttggccaa taccgtcatc gacatcgcac
     4141 tcacgcagat ccggtcatgg cgccgagaag gaatcactct caccgtcgcg gtaaacctgt
     4201 cgcccacgaa tctgctcgac ctagacctcg tcgacaccgt cgagcggctg cttgccaccc
     4261 atggcctacc cgcggaggcg ctcatcgtcg aaatcaccga aagcaccctc gccaccgact
     4321 cggtgcggtc gcgcagcacg gtcgcggcgt tgcgcagact cggcgtacgc atctcgctcg
     4381 acgactacgg aaccggctgg tcgtcgcttg cgcggctgca ggatctgtcg gtcgacgagc
     4441 tcaagctcga cagggtcttc gtttcccgga tcgcccagga tcccaggtcg atggcgatcg
     4501 tcagatcgac cgtggcgctt gcgcacagcc tcggcgccga cctcgtcgca gaaggagtcg
     4561 aagacgaggc gaccctgggc gctctgcggc gatacggttg caacatcacg cagggctacg
     4621 tccacagccc gccgctaccg gccaacgagt tcgagcagtg gatcgctgag tacacaccca
     4681 ccgtcaacta cccgcacgct gtggcgaccg actgatttgg ccgcaagccg cggcttcggt
     4741 gaccttgcgt ttgccggtgc ggggagtcca ctcgccagca aagatgcgtt ttcatgatcg
     4801 agaagcgggt taactttgcg gacgcggcaa aggcgccgca gcgatcggag cgcgcatgac
     4861 acagcaactg agccgtcgca gcaaactcgt gggcgggtcg tccaacgggc ttgcccagtt
     4921 gccggccggt cgctcgatca tggtgcgttc cgccgacggc acccggctgc acaccgaaat
     4981 cttcggccct gaagacggct acccgatcgt gttcagtcat ggcatcacct gcgcgctgcg
     5041 ggttctggca caaccagatc gccgacctgg tggccgacca ccgggtgatt gcgttcgacc
     5101 accgcggcca cggaaaaagc gaagtccccc gtcggcgcgg ctacagatcg accatttggc
     5161 ggacgacttg cacgcggtcc tcgaggcgac actacgaccg ggggagcgcg cggtgatcgc
     5221 tggccattcg atgggcggca tcacgatcag cgcgtggtcg gaccggtacc gccacgccgt
     5281 gccgcaccgc gccgacgcgg tcgcgttgat caacaccaca acgggcgacc tattgcggga
     5341 aatccaactg attcaggtgc cgggtatgct cgccgcgacc cggattcgca tcgcggagca
     5401 ggtgattagg accattggct cgcagaaggt cccacgcggc gcgaagtgga caagccgcag
     5461 gttcgtcgcg gcgatcgccg taggggccgg cgcgcacccc gcagtctaga tctggtccat
     5521 gagcttttcg ccgccaccgc gcccgtcgcc cgcggcagct gcatgaggat gctcacggcc
     5581 gcccttgggc cacaacatct cccgttggat ggcctcacgg tgccggcgtt cgtgatcggt
     5641 agctcgaaag accggctgtt gccgatgtgt cagtctcgaa agatcgctga tgccgtgccg
     5701 agtctggcgg ggcttgtcga gcttccgggt ggccattgtg cgatcctcga gcggcccggc
     5761 cggcaccccg tgtgcctcgt cgtactcaca gtcgttcgcc tggggtgttc gcgttctcgg
     5821 cacccaggtc gcgagcgggc cggttctctt ccacgtccag ccactgtcga tgggtgcgcg
     5881 actggctgcc gagcggtcgc cctctagctc cgcgactgac gaccggccca tcgttcttgt
     5941 gtgtgaacga atttgtgaac gaaacacggc gcaactgccg agatggcgtg gaaacgcggc
     6001 gcgatggttt ggacgggcga ttgatcgtga cctgcaggtt tgggacacct gaggtgacgc
     6061 gagaccgcga ccggtcggtg tcatttgcca cggggatggg accaccgaat tgccacgagg
     6121 atgggaccac cgggttgtct catcgagcat cctggtctgg gttggtggtg tcaaccggaa
     6181 cgtgtgggtt tttggcggcg ccggtaggac tcgccttcga tgacgagttc gtgtgcggtg
     6241 gaggtgagcc ggtcgacagc ggattgggcg agcagcgggt cgctcatcat ggtcagccat
     6301 tccgccggtt cgcggttgga ggtcgtcacg gtggaggcct tgtggtggcg ggccacgacg
     6361 agctcgtaga agtcggcggt ttcggtggcg tcgagcggct gcagcgcgaa gtcgtcgatc
     6421 agcagcagct ggacttgggc gagccggcgc atctcggcct cgacgctgtt gtcgaggcgg
     6481 gcagccttga gccgcttgaa cattgcatca gcgcgcagca tgagcacgct gacccggcgg
     6541 cgcacggcga tgtggccgag cgcggtggcc aggtgggttt tgcccacgcc caccgggccc
     6601 agaaggcagg cgccgtgcgg gccgtcgagg aaccgcaggc tggtcagctc gttccacagg
     6661 gtgcggtcgt agcggaccgc ggcggtgtcg tcccaggtgt caatccgcat gccggggtcg
     6721 agcccggcgc cgcgggcgcg ccgggcggcg gagctggtgt cgcggcgggt ggcctcgtcg
     6781 gcgaggacga gttcgaggaa cgccacgtgc gacaggtgct gctggcgggc cagggtgagc
     6841 cgctcgggca gggtgtcgag catccggccg agcttgagct gccgcagcac ctgcttcagg
     6901 tcgggtgaga tcgggtcatg gatgacagtt ttcgtcactg ctgggtctcc gtcccggctc
     6961 cgccgtcgat caccctcagc cagtccggtg tggttctgtt cgtccggtat tcggtggcgt
     7021 cgcgggcgaa ccttgccggg acggcggccc gagcggcgcg gggcgccggg gtcggggtgt
     7081 tctcggtggc tttctccagc atggcggcga tcttggcgac ggagacgacg tcgaggtcaa
     7141 gagcgcgccc gcacgcggtg tcgactggct cgctgccgta gcgtttggcc agccccaaca
     7201 gccgatacgc ctgccgcatc ttggtccacg gcagctggtg gtcgagcagc cgttcggcgt
     7261 agatgccgac gttgggcccg tgcccgcggg cggcgctgat cagccgctgc aggtcgcgca
     7321 tggcgtaggc ggttttctcc gcgggcaggt cctcggggtc ggtggatcgg cggcccggtg
     7381 cttggcgcgg gtgggtctta accagctgac cgcggcggaa cagcttcacc agctcgtcgt
     7441 ccgctctggc ttgaatccgt tgcccgagaa ggtgttccgg tgtcgagtac aatgccctgc
     7501 cgacttggac gtggtagtcg cggtggacgg tgacctcggt gtagatcggc acctggtagg
     7561 tcacgtcggg cgccggcagc agcaccgcgg cctcgtgttc ggcgaacacc accgccggtt
     7621 gcgcgcaggt ggtgccgtgg atgcgcagcc ccgccttccc cgtgcaccag gtcgtcgcgc
     7681 gggcctgcgc gtcggccagg tcgacgaact cctcaccggc gaagaagttg ccgcgcacgt
     7741 actgcactac ccgctccacc cgaggcttat cctgggggct ggcaacacgc gccgggtctg
     7801 tcgcgaaacc acgcgcctgc gcgtactcca gccaccccac agtgaaccgc gggttgatcg
     7861 catccgcctg ggccacaatc ggtttcatgt tgtccgggac gagcaccttg aacacgccgc
     7921 cgaagaagcg ccaccccgcc tcacagccgg cgatgatcgc ctccagcgtc tgggagaacg
     7981 tcagccacac gaacatgtgc cgcgagtaca ccgcggtgaa gatcaacgcg tgcaccaccc
     8041 gccgccgccc gacctgcggg tcgtagatca aacccattct ggcgaaatcg atctggcact
     8101 ccacaccggg ctccccgtct gccaccggca ccgtgtcccg gcggccccgg tactcggtgc
     8161 gctcgacgca gaaccggtgc agcgtgcggt gcggcaccac cacgccctct ctggcaagca
     8221 ggtctgcgat cttcaccacc gtcaagccct gaccgaccca cctgacgatc cgatcacgct
     8281 gcccttcaag ggattcccac gccaaaccat gaccgttcgg gcggtccgga cgcaccgccg
     8341 cgaccacctg cccgatcagc tcgtcggtca actgctcctc gccgccgtcg cggtccaaac
     8401 cggccacgac cgcggcctcc acgttacgca ccacggtctt gcggtccacc cccgcctgcc
     8461 gggaccaacc tggtcaaaat cggcgccctc gcc
//
LOCUS       DASWBF010000496         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1098750,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000496 DASWBF010000000
VERSION     DASWBF010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098750"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..687)
                     /locus_tag="VF874_18205"
     CDS             complement(226..687)
                     /locus_tag="VF874_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177918.1"
                     /translation="MCPTLLQARQFAVAAHQRTPRDSGRLDPLADHLVAIDPLNTLHG
                     GGGAGLQPARLMTGLLGGANYSMNQANDDLARLRLNALITRVPGRNRYRLTGDGLRFA
                     IFYAKLHDRLLRPLLAADQLPAPPPLRKALHTIDIHITDHIDQARLMPNAA"
     gene            complement(802..1611)
                     /locus_tag="VF874_18210"
     CDS             complement(802..1611)
                     /locus_tag="VF874_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9177919.1"
                     /translation="MSVDETRGKAVTVTAGAGLPLHRQLFLVLHDEIARGAMPAGDPL
                     PTEQELCDQFGVSRITVRRALADLAAQGYITRRQGVGSFVREHHPTARQANTRSYMDE
                     LRQIHFETGAAVLEFDLRAAPQTICDKVGFAGQVLHILRVRRERRTGEPLLVTEAWLP
                     AELAEVITAEMLLDTPMYRLLADAGITLDRLQHEFTAEIAGPRNAQLLDIAIGAALMR
                     VNRLAFIGDRPHHYLSMLLSPSRSRILLNQSADELETGATMAITHDVRRDT"
     gene            1740..2009
                     /locus_tag="VF874_18215"
                     /pseudo
     CDS             1740..2009
                     /locus_tag="VF874_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(2182..>2390)
                     /locus_tag="VF874_18220"
     CDS             complement(2182..>2390)
                     /locus_tag="VF874_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177920.1"
                     /translation="GAFIHWVSADGHGVVSQDFRDLAAVGRTRRDAAQLARVLCTCAA
                     RRDLNTRNSYSHRVTAAWVAKGHR"
BASE COUNT          409 a          721 c          839 g          421 t
ORIGIN      
        1 atgttgtgcc gcaacggcga tggtaaacgc agatacctcg tctcgagccg agaccaaagg
       61 gcgcagccat cggcagattg acgcgaggga tccggggcgc ggcggtcgga gctaaacgcc
      121 agaccgctat tttgtgttgc ccgcagggcc accgctgccg ggcgaagtcc cggcggcggc
      181 gtagcgaacc ttggtcgcta ggacgttaag agttgtctcg agttttcagg ctgcgttcgg
      241 catgagtctg gcctggtcga tgtggtctgt gatgtggatg tctatggtgt gcaatgcttt
      301 tcgtaatggc ggtggtgccg gtagctggtc ggcggccagg agcgggcgca gcagccggtc
      361 gtggagcttg gcgtagaaga tcgcaaaccg aagtccgtcg ccggtgagcc ggtagcggtt
      421 gcggccgggg actcgggtga tgagagcgtt gagtcgtagc cgcgccaggt cgtcgttggc
      481 ctggttcatc gagtagttcg caccgccgag cagtccggtc atcaggcgcg caggctgaag
      541 gcccgccccg ccgccgccgt gaagggtgtt gaggggatca attgccacca ggtgatcggc
      601 gagcgggtcg agccggccag aatcacgcgg ggtccgctgg tgggccgcga ccgcgaactg
      661 gcgcgcctgc agaagagtcg ggcacacgcg cgggccggtg ccgccgccct tcggccgcgg
      721 cgagcactgc agcgacctga ccggtctgga gctgtgggga agggctgacc catcttcaat
      781 ttggcgggtt ggcctgctcg gctaggtgtc gcggcgcacg tcgtgcgtga ttgccatggt
      841 ggcaccggtt tcgagttcgt ctgcggattg gttgagcagg atcctgcttc ggctgggcga
      901 cagaagcatt gagagataat ggtggggtcg atcgcctatg aatgcgagtc ggttcacgcg
      961 catcagtgcg gcaccgatgg cgatgtcaag tagctgggca ttgcgggggc cggcgatctc
     1021 ggcggtgaac tcgtgctgca ggcggtccag ggtgatcccg gcgtcggcaa gcaggcgata
     1081 catcggcgtg tcgagcaaca tttcggcggt gatcacctcg gcgagctcgg cgggaagcca
     1141 ggcttcggtg acgagcagtg gttcaccggt tcggcgctca cgccgtaccc gcaagatgtg
     1201 taacacctgg ccagcgaatc cgaccttgtc acagatggtt tggggcgcag cccgcagatc
     1261 gaattcgagg accgcggcgc cggtttcgaa gtggatctgg cgcagctcat ccatgtagga
     1321 ccgggtgttg gcctgccgtg cggtcgggtg gtgttcgcga acgaaggatc cgacgccttg
     1381 ccggcgggtg atgtaaccct gagcggccag gtcggccaat gcccgccgga cggtgatgcg
     1441 ggagacaccg aattgatcgc acagctcctg ctcggtgggc agggggtcac ccgccggcat
     1501 ggccccgcgc gcgatctcgt cgtgcaacac caggaacagc tgccgatgca gcggcaggcc
     1561 cgcgccggcc gtcaccgtca cggccttgcc gcgggtttca tcgacgctca ccggagaccc
     1621 cgcttacggt cgccgtcgat gactaccagc tcttctccag ccaagtgtta cccaatcgcc
     1681 aaagttatgc tgttagtata acaacaagat gtctccagtt gtcgagttga ggcatccgag
     1741 aggtcatcaa cgaccgtggc agcagagcaa gccgcggccg actgcgcacc gaggaggcgc
     1801 ccatggacgc actggaccgg atgctgatcg gtgaagcgct ggcacatttg tccgcacagg
     1861 accgcgccgt gattcgccgc tcctactacc agggctgcac gaccacgcag atcgccgacg
     1921 atctccagat cgccgaaggc gccgtgaagt cgagactgca ctacgccgtg cgagcgctac
     1981 ggctcacatt gcaggacatg ggggtgacat gtgacgcgac tgcggtcagc gcgtgtgcgg
     2041 tgcccaacac cgccgtagtg cacgaggggg gcgccgcccg ccgacggtgc cgtgatgtcg
     2101 ggcctcatgg aatcacaccc ggctcggccg gtcacccaat aggctgacta atcggcccgc
     2161 taatcggccc ggatgatccg gtcaccgatg gcccttggcg acccaggcgg ccgttacccg
     2221 gtgactgtac gagttgcgcg tgttcagatc gcgacgcgcc gcgcacgtgc agagcaccct
     2281 ggccagttgg gccgcatcac gccttgtccg accgacggcc gccagatcgc ggaagtcctg
     2341 cgacaccaca ccatggccgt ccgcggacac ccagtggata aatgcccctt
//
LOCUS       DASWBF010000497        10886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_712249,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000497 DASWBF010000000
VERSION     DASWBF010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712249"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VF874_18225"
     CDS             complement(<1..707)
                     /locus_tag="VF874_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9177921.1"
                     /translation="MATDDQGAAQAVQTPPAPETPAPAPAAPKSSSASRRVRARLARR
                     MTAQRSAVNPVLEPLVAVHKEIYPKADLTMLQRAYEVAEERHATQFRRSGDPYITHPL
                     AVANILAELGMDTTTLIAALLHDTVEDTGYTLEALTADFGEELGHLVDGVTKLDKVVL
                     GSAAEGETIRKMVIAMARDPRVLVIKVADRLHNMRTMRFLPPEKQARKSRETLEVIAP
                     LAHRLGMATVKWELEDLS"
     gene            complement(737..1282)
                     /locus_tag="VF874_18230"
     CDS             complement(737..1282)
                     /locus_tag="VF874_18230"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX9177922.1"
                     /translation="MSDTSGDQTRIVEVITSLIREVPDFPSPGVLFKDLTPLLADPDG
                     LAAVTDALSELASGAELVAGIDARGFLLGAAVATRLGTGVLAIRKGGKLPPPVLSETY
                     TLEYGTATLEIPADGVELAGRNVVIIDDVLATGGTLAAAARLLDDAGAAVTSAAVVLE
                     LAELRGRDAVAPLRVSSLYQV"
     gene            complement(1279..2916)
                     /locus_tag="VF874_18235"
     CDS             complement(1279..2916)
                     /locus_tag="VF874_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9177923.1"
                     /translation="MRHRTAVFAAAALLVAAVLLTSCSNKPAEQLDYAVDGPLPTYNP
                     NTVVGAASAAPQALARVLTGFGYHGPDGQIVADHDFGTVSVVGREPLLLDYQIADPAV
                     YSDGKPVTCDDMVLAWAAQSGRLPGFDAASRAGYADIESIDCTPGQKKARVTFLAGRS
                     FTDYPQLFTATSMMPSHVLADLLGVDVTAALQNDDAPVVARIADAWNTVWDLKPGINL
                     RWFPSSGPYKVDSVHSDGSVVLVANERWWATKPVTNRITVWPRGADVQQRLKDGSVDV
                     VDVATGSVGGLSVPDGYQRTDTSSAGIQQLVFSGQGPLAVPAARRAVASCTPRDTIAG
                     DVGVPVSNARLNPANDDAFGAAEDAAANNLYVAANADAARAALNNTPLTVRIGYQAPN
                     ARLNAVVSAIAKACAPAGITVQDVSSEPTGPQTLQANEIDALLASTGGATGSGSSGSS
                     AIDAFDLHTGNGNNLSGYSNPQVDGIIDKLAATSDPKEVARLLGDGASVLWNDMPTLP
                     LYRQQRMLVSSKKMSAVSSNPTRWGAGWNMDRWVLKE"
     gene            complement(2916..4178)
                     /gene="secF"
                     /locus_tag="VF874_18240"
     CDS             complement(2916..4178)
                     /gene="secF"
                     /locus_tag="VF874_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX9177924.1"
                     /translation="MAKASEDKTLADAELLESEAVEETFDSTGPRHGFFIRLYTGTGA
                     FEVVGRRKMWFAVSGVIIAIAIASIAIRGFNFGIDFEGGTKVSFPRGEATVQQVEDVF
                     RTTLGSEPEQVVTVGNGASATVQIRSEMLSNDQTAKLKDALFNEFQPVGADGKPSKQA
                     ISDSAVSSTWGGQITKKALIALGVFLVLVSLYITIRYERYMAISALVTMFFDIITTAG
                     VYSLVGFEVTPATVIGLLTILGFSIYDTVIVFDKVEENTHGFQHTTRRTFAEQANLAV
                     NQTFMRSINTSLISVLPIASLMVVAVWLLGVGTLKDLALVQLVGIIVGTYSSIFFATP
                     LLVTLRERTELVRTHTRRVLRRRKAAGADVPEAASEETTADARSKRGSDEPSAREPVA
                     LAAKPAPGARPIRPTGSRAARPSGKRRR"
     gene            complement(4181..5905)
                     /gene="secD"
                     /locus_tag="VF874_18245"
     CDS             complement(4181..5905)
                     /gene="secD"
                     /locus_tag="VF874_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX9177925.1"
                     /translation="MASSSPVHPFRYLSLFLILLIGTYLLMFLTGNKEAKPKLGIDLQ
                     GGTRVTLTARTPDGSRPTREALDQAQQIISARVNGLGVSGSEVVVDGDNLVITVPGTD
                     GNEARNLGQTARLYIRPVIDVMQIPPEGEQPPGATPGLPPGAIPGIPGVEPAPPPAEA
                     PPPQTAPPAQPAPQPRLYPEEPPPPTPPAPPPPPAPPPAPPDPRKDLADAIAFQKALR
                     QNPQLSQIALSVLMESRCQGPDPLAGNDDPQQILVTCSQDRTAIYLLDKSIISGDQIA
                     SASSGFDQQGSRWVVNVKFKPDAADTWADYTAAHVGTQTAFVLDSQVVSAPVINEAIP
                     GGNTQITGDFTSAKARDLANVLKYGSLPLSFESSEAETVSATLGLTSLKAGLIAGAIG
                     LALVLVYSLLYYRVLGLLTALSLVLSGAMVFAILVLLGRYINYTLDLAGIAGLIIGIG
                     TTADSFVVFFERIKDEIREGRSFRSAVPRGWARARKTIVSGNAVTFLAAAVLYFLAIG
                     QVKGFAFTLGLMTILDLVVVFLVTWPLAYLASKSAVMAKPAFNGLGAVQQIARERRAA
                     AHATTGQR"
     gene            complement(5956..6282)
                     /gene="yajC"
                     /locus_tag="VF874_18250"
     CDS             complement(5956..6282)
                     /gene="yajC"
                     /locus_tag="VF874_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX9177926.1"
                     /translation="MEQLIIFLPLLVIMGAFMFFASRRQKRAMQATIDLHESLRVGDR
                     VHTTSGLQATIAGITDDTVDLEIAPGVITTWMKLAIRDRVEPDIDDDDDEITAEADEP
                     GRLTQD"
     gene            6415..7767
                     /gene="gabT"
                     /locus_tag="VF874_18255"
     CDS             6415..7767
                     /gene="gabT"
                     /locus_tag="VF874_18255"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX9177927.1"
                     /translation="MTDLEQSRMLVTEIPGPASLELINRKAAAVPRGVGNTMPVYVAR
                     AGGGIVEDVDGNRLIDLGSGIAVTTIGNAAPRVVDAVRAQAADFTHTCFMITPYEGYV
                     AVAEQLNRLTPGDGPKRSALFNSGAEAVENAVKIARAFTRKTAVVAFDHAYHGRTNLT
                     MALTAKSMPYKSGFGPFAPEVYRAPLSYPYRDGLLSKELATNGELAAARAITSIDNEV
                     GAENLAAVVIEPIQGEGGFIVPADGFLPALSAWCRDNDVVFIADEVQTGFARTGKMFA
                     CEHEGIEPDLIVTAKGIADGLPLSAVTGRAEIMDAPHISGLGGTYGGNPIACAAALAT
                     IETIEADGLVARAADIENVMTDALGRMQAEDDRIGDVRGRGAMIAMELVKPGTAEPDP
                     VLTRTLSTRAHAAGVIVLTCGTFNNVVRFLPPLPISDELLVEGLDVLASILAETANPR
                     "
     gene            7823..9256
                     /locus_tag="VF874_18260"
     CDS             7823..9256
                     /locus_tag="VF874_18260"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX9177928.1"
                     /translation="MTTVQNFIDGKLVNSVSGATMPLVDPATGEKYGTAPVSNEEDID
                     NAYAAAARAFSDWKRTTPAHRQKALLDFADEVEQAARALVEAEGRNTGKPNHVTMAEE
                     IPPMVDQIRFFAGAARILEGKSAGEYMEGHTSWIRREPVGVIGQVTPWNYPMMMAIWK
                     FAPAIAAGNTVVIKPSDTTPVTTVMLAEMASKHLPPGVLNVVTGDRVTGAALVAHPTP
                     QMVSITGSVAAGRAVAVSAGGNLKRTHLELGGKAPVIVFPDADIAAAAEGIATAGYFN
                     AGQDCTAATRVIGHAAVANDLTDALAEQARSAATTFGRAADDEDAWVPPVNNANQLQR
                     VLGFLAEVPVHATVAVGGGRQGERGYYVEPTVIGGLGQDDRLSQNEIFGPVITVQSFD
                     DEDDVVRLANGVEYGLASSVWTKDVSVALRVSAALDFGCVWINTHIPLVAEMPHGGFK
                     SSGHGKDLSMYGFEDYTRVKHVMARIS"
     gene            9516..>10886
                     /locus_tag="VF874_18265"
     CDS             9516..>10886
                     /locus_tag="VF874_18265"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177929.1"
                     /translation="MTTAVGLDRETLEMMLSSLDEFVAETLPDQRRLDLDHEDVCPED
                     TVREMCSEELGVQLVFIPEEYGGLGGGAFDSYRVCERLARVDIGVATSVFATFLGSDP
                     ILVGATDEQRKEWLGRIAEQGIIFAYGATEPEAGSDLGAMTTTAHPIETDGEVTAYRI
                     NGRKQWISNGSIADVYSILALAPGGPSWFVVEKGTPGFSTAPPEDKHGIRLSNTAALF
                     LDDVVVPAENLVGREEGRGLVQAQQVFGYTRVMVGAFGLGGGWEALDRAIAYSENRVQ
                     GGAPLSTKQGYTHKLIVPHAVRLEAARAFLEETASRIDAGEGGAGALNTEGAIAKLFA
                     TESGNAAAEAAIQAHGGYGYTRPYLVEKIKRDVRITTIYEGTSEILEMTIARDRWQQH
                     LKTSGAYYRDAARALDGVPDEYGGPTAALALECLASVLEACRVGRLTRNQHVLLRLGE
                     LVTYAEC"
BASE COUNT         1786 a         3651 c         3766 g         1683 t
ORIGIN      
        1 gacaggtcct cgagctccca cttgaccgtc gccataccga gccgatgtgc cagcggcgcg
       61 atgacttcca acgtctcacg cgacttgcgg gcctgcttct ccggcggcag gaaccgcatc
      121 gtgcgcatgt tgtgcagtcg gtcggcgacc ttgatcacca gcacgcgggg atcgcgggcc
      181 atcgcgatca ccatcttgcg gatggtctcc ccttcggcgg cgctgcccag caccaccttg
      241 tccagcttgg tgaccccgtc gaccagatgc ccgagctctt cgccgaaatc ggcggtcagc
      301 gcctccagcg tgtagccggt gtcctcgacg gtgtcgtgca gcagcgcggc gatcagcgtg
      361 gtggtgtcca tgccgagctc ggccaaaatg ttcgcgacgg ccagcggatg ggtgatgtac
      421 ggatcgccgg agcggcggaa ctgggtggcg tgacgttcct cggcgacctc gtatgcccgc
      481 tgcagcatcg tcaggtcggc cttcgggtag atctccttgt gcaccgcgac cagcggctcg
      541 agcacgggat taacggcgct gcgctgggcg gtcatccggc gcgccaggcg ggcgcgcact
      601 cgccgcgacg cgctggagga cttcggcgcc gcaggtgccg gcgcgggagt ctccggcgca
      661 ggcggcgtct gaacggcctg ggctgcgcct tggtcgtcag tcgccatcag tacacctccc
      721 gtaccgagga taaacgctag acctggtaca agctgctgac ccgcagcggc gccaccgcat
      781 cgcggccgcg cagttcggcg agctccagca ccaccgcggc gctggtgacc gctgcgcccg
      841 cgtcgtctag cagccgcgcg gccgccgcca gggtgccgcc ggtggccagc acgtcgtcga
      901 tgatcaccac gttgcggccc gcaagctcca caccgtccgc cgggatctcc aacgtggccg
      961 tgccgtactc gagcgtgtag gtctcgctca acaccggcgg cggcagcttc ccgcccttgc
     1021 ggatggccaa taccccagtg cccagccggg tcgcgaccgc cgctccgagc aggaagccgc
     1081 gcgcgtcgat gccggcgacc agttccgcgc cggaggccag ttcagacagc gcgtcggtga
     1141 ccgcggccag tccgtccggg tcggccagca gcggcgtcag gtccttgaac aggacaccgg
     1201 gcgacgggaa gtcgggcacc tcgcggatca gcgacgtgat gacctcgacg atccgcgtct
     1261 ggtccccgct ggtgtcgctc attccttcag cacccatcgg tccatgttcc agcccgcgcc
     1321 ccagcgggtc gggttgctgc ttaccgccga catcttcttc gacgagacca gcatgcgctg
     1381 ctgccggtac agcggcaacg tcggcatgtc gttccacaac actgaagcgc cgtcacccaa
     1441 cagccgggcg acctctttag ggtcggaggt cgcggccagc ttgtcgatga tgccgtccac
     1501 ctgcggattg gagtagccgg agaggttgtt gccgttgccc gtgtgcaggt cgaacgcgtc
     1561 gatcgccgac gacccactgg agccgctgcc ggtcgctccg ccggtgctgg ccagcagcgc
     1621 gtcgatctcg ttggcctgca acgtctgcgg gccagtgggc tcagacgaca cgtcctgaac
     1681 ggtgatgccg gccggggcgc aggccttcgc gatcgcgctg accaccgcgt tcagccgggc
     1741 gttgggcgcc tggtagccga tccgcaccgt gagtggcgtg ttgttcagcg ccgcgcgggc
     1801 ggcgtcggcg ttggccgcga cgtacaggtt gttcgcggcg gcgtcttcgg ctgccccgaa
     1861 cgcgtcgtcg ttggcggggt tgagccgggc gttcgagacc ggcactccga catcgccggc
     1921 gatcgtgtcg cgcggggtgc acgaggccac cgcccggcgc gccgcgggaa cggccagcgg
     1981 tccctgcccc gagaagacga gttgctggat gccggccgac gaggtgtcgg tgcgctggta
     2041 gccgtcggga accgacaagc cgccgaccga ccccgtcgcg acgtcaacca cgtccacgga
     2101 gccatccttg aggcgctgct gcacgtcggc gccacgaggc cacacggtga tgcggttggt
     2161 gaccggcttt gtcgcccacc accgctcgtt cgccacgagc accaccgagc cgtccgagtg
     2221 cacggagtcc accttgtatg ggcccgacga cgggaaccac cgcagattga taccgggctt
     2281 cagatcccac accgtgttcc atgcgtcggc gatccgcgcc accaccggcg catcgtcgtt
     2341 ctgcagcgcg gcggtgacgt cgacacccag caggtcggcg aggacgtgcg acggcatcat
     2401 cgacgtggca gtgaacagtt gcggatagtc ggtgaacgag cgcccggcga ggaacgtcac
     2461 gcgggccttc ttctggcccg gcgtgcagtc gatggattcg atgtcggcgt atcccgcccg
     2521 gctggccgcg tcgaaaccgg gcagccggcc tgactgcgcc gcccatgcca gcaccatgtc
     2581 gtcacaggtc accggcttgc cgtcggagta caccgcggga tcggcgatct ggtagtccag
     2641 cagcaacggc tcgcggccca cgacggacac cgtgccgaag tcgtgatcgg cgacgatctg
     2701 cccgtccggg ccgtggtatc cgaagccggt gagcactcgg gccaacgcct gcggtgcggc
     2761 cgatgcggcg cccaccacgg tgttcgggtt gtaggtgggc agcggcccgt cgacggcgta
     2821 gtcgagctgc tcggccggct tattcgagca cgacgtcagc aagacagccg ctaccagcag
     2881 cgcggccgcg gcgaagacag ccgtccggtg gcgcactagc gccgccgctt gcccgagggc
     2941 cgggcggcgc gggagcccgt cgggcggatc ggccgcgcgc cgggagccgg cttcgcggct
     3001 agcgccactg gttccctcgc gctgggctca tcgctgcctc tcttgctcct cgcgtccgcg
     3061 gtggtctcct cgctggcggc ctcgggcaca tcggcaccag ccgccttgcg gcggcgcagc
     3121 acccggcggg tgtgggtgcg gacgagctcg gtgcgctcgc gcagagtcac caaaagcggg
     3181 gtggcgaaga agatcgacga gtaggtgccg acgatgatgc cgaccagctg caccagcgcc
     3241 aggtccttca acgtgccgac accgagcaac cacaccgcga ccaccatcag cgaggcgatc
     3301 ggaagcaccg agatcaggct ggtgttgatc gaccgcatga acgtctggtt gaccgcgagg
     3361 ttggcctgtt cggcgaacgt ccgccgggtg gtgtgctgga agccgtgcgt gttctcctcg
     3421 accttgtcga acacgatcac ggtgtcgtag atggagaagc ccagaatggt gagcagtccg
     3481 atgacggtgg ccggggtgac ttcgaagccg accagggagt acaccccggc cgtggtgatg
     3541 atgtcgaaga acatcgtcac cagcgcggag atcgccatgt agcgctcgta gcggatcgtg
     3601 atgtagaggc tgacgagcac caggaacacg ccgagcgcga tcagtgcctt cttggtgatc
     3661 tgcccgcccc acgtcgacga caccgcggag tcgctgatgg cctgcttgct gggcttgccg
     3721 tcggcgccga ccggctggaa ctcgttgaac agcgcgtctt tcagcttcgc ggtctggtcg
     3781 ttggacaaca tctccgagcg gatctgcacg gtggcggagg cgccgttgcc gacggtgacc
     3841 acctgctcgg gttcggagcc gagcgtcgtg cgaaacacgt cctcgacctg ctgcaccgtg
     3901 gcttcgcccc gagggaacga caccttggtg ccgccctcga agtcgatacc gaagttgaag
     3961 ccgcgaatcg cgatgctcgc gatcgcgatc gcgatgatca cgccactgac cgcgaaccac
     4021 atcttgcgtc gccccacgac ctcgaacgca ccggtgcccg tgtagaggcg gatgaagaag
     4081 ccgtgccgcg gtccggtcga atcgaacgtc tcctcgaccg cctccgactc aaggagttcg
     4141 gcgtcggcga gtgtcttgtc ctcggaagcc ttggccatgc ctacctctgt cccgtcgtcg
     4201 catgcgccgc cgcacgacgt tcccgagcga tctgctgaac ggcgccgagg ccgttgaatg
     4261 caggtttggc catcaccgcc gacttcgacg cgagatacgc cagcggccac gtcaccagga
     4321 acaccaccac caagtcgaga atcgtcatca gaccgagtgt gaatgcgaag cccttcacct
     4381 gaccgatggc caggaagtac agcaccgcgg cggccaggaa ggtcacagcg ttgccagaca
     4441 cgatcgtctt ccgggcccga gcccagccac gcggcaccgc cgatcggaac gaacggcctt
     4501 cgcggatctc gtctttgatc cgttcgaaga acacgacgaa cgagtccgcg gtggtgccga
     4561 tgccgatgat cagaccggcg atgcctgcca ggtcgagcgt gtagttgatg tagcggccca
     4621 gcaagaccag gatcgcgaac accatcgcgc cggacagcac cagcgacagg gccgtgagca
     4681 agcccagcac gcggtagtag agcagcgagt acaccagcac gagcgcaagt ccgatggcgc
     4741 ctgcgatcaa gccggccttc agcgacgtca aacccaatgt cgccgaaacg gtttcggcct
     4801 cggacgactc gaacgacagc ggcagtgacc cgtacttgag cacgttggcc aggtcacggg
     4861 ccttcgcgga ggtgaaatcg ccggtgatct gggtgttgcc gccggggatc gcctcgttga
     4921 tcaccggtgc gctgaccacc tgcgagtcga gcacgaacgc ggtctgggtg ccgacgtgcg
     4981 cggcggtgta atccgcccag gtgtcggctg cgtcgggctt gaacttgacg ttcaccaccc
     5041 accgggagcc ctgttgatcg aatcccgaac tggcgctggc gatttgatca ccgctgatga
     5101 tcgacttgtc caacagatag atcgctgtgc ggtcttgcga gcacgtcacc aggatctgct
     5161 gcggatcgtc gttgccggcc agcgggtctg ggccctggca tcgcgactcc atcagcacgc
     5221 tgagcgcgat ttgggagagc tgcggattct gccgcagcgc cttctggaag gcgatggcgt
     5281 ccgccaggtc tttgcgcgga tccggcgggg ccggtggcgg cgccggcggt ggcggcggtg
     5341 ccggcggggt gggcgggggc ggctcctcgg ggtacagccg cggctgcggg gccggttgtg
     5401 cgggcggggc ggtctgcggc gggggcgcct cggccggcgg cggagcgggc tcgaccccgg
     5461 ggatgcccgg tatggcgccg ggcggcagcc ccggtgtggc accggggggc tgctcaccct
     5521 ccggcgggat ctgcatgacg tcgatgactg gccggatgta cagccgggcc gtctggccga
     5581 ggttgcgcgc ctcgttgccg tcggtgcccg ggacggtgat caccaggttg tcgccgtcga
     5641 ccaccacctc cgaccccgac acgccgagac cgttgacgcg cgcactgatg atctgctgcg
     5701 cctggtcaag ggcctcgcgc gtcggccgcg acccgtcggg agtgcgggcc gtgagcgtca
     5761 cccgggtgcc accctgcaag tcgatgccga gcttcggctt ggcctccttg ttgccggtca
     5821 ggaacatcag caggtaggta ccgatcaaca gaatcaagaa cagcgacagg taccggaatg
     5881 ggtgcaccgg cgacgacgat gccacggtcc tttagtctcc tcgggtttgt tacggctgca
     5941 gacagtacgt gccgctcagt cttgagtgag tcggcccggc tcgtccgcct cggcggtgat
     6001 ctcgtcgtcg tcgtcgtcga tgtccggctc gacgcggtcg cggatcgcca gcttcatcca
     6061 cgtggtgatc acaccgggcg cgatctccag gtcgacggtg tcgtcggtga tgccggcgat
     6121 tgtggcctgc agcccggagg tggtgtgcac ccggtcgccg actcgcagcg attcgtgcag
     6181 gtcgatggtg gcctgcatcg cgcgcttctg gcgccgcgac gcgaagaaca tgaacgcgcc
     6241 catgatgacg agtagcggga ggaagatgat cagctgttcc atgagatctt tcgtgtagtg
     6301 cagatggtcg ggccccacag gcggggcctc gcaactgacc agtgtgccat ccgcccacga
     6361 gacggccgtg cagcactagg ctcagcctcg ggaggggctg cctgaggaga gccgttgacc
     6421 gacctcgaac agagccgcat gctcgtcacc gagatccccg gtcctgcgtc actggaactg
     6481 atcaaccgca aggccgcggc cgtgccgcgc ggggtcggca acacgatgcc ggtctacgtc
     6541 gcccgcgccg gcggcggcat cgtcgaggac gtcgacggca accggctgat cgacctgggc
     6601 tcgggcatcg cggtcaccac gatcggcaac gccgcccccc gggtcgtcga cgcagttcgc
     6661 gcgcaagccg ccgacttcac ccacacctgc ttcatgatca cgccctacga gggctacgtg
     6721 gcggtggccg agcagctcaa ccggctgacc cccggcgacg gtcctaaacg gtccgcgctg
     6781 ttcaactccg gcgcggaggc cgtggagaac gcggtcaaga tcgcccgcgc gttcacccgc
     6841 aagaccgcgg tcgtcgcgtt cgaccacgcc taccacgggc gcaccaacct gacgatggcg
     6901 ctgaccgcga agtcgatgcc ctataagagc ggcttcgggc cgttcgctcc ggaggtgtac
     6961 cgggcgccgc tgtcgtatcc ataccgcgac gggctgctct ccaaggagct ggccaccaat
     7021 ggcgagctgg cggccgcgcg cgcgatcacc tcgatcgaca acgaggtcgg cgcggaaaac
     7081 ctcgccgcgg ttgtgatcga gccgatccag ggcgaaggcg gcttcatcgt gccggccgac
     7141 ggcttcctgc cggcgctgtc ggcctggtgc cgcgacaacg acgtggtgtt catcgccgac
     7201 gaggtgcaga ccggcttcgc ccggaccggc aagatgttcg cctgcgagca cgagggcatc
     7261 gagccggacc tgatcgtcac cgcgaagggg atcgccgacg ggctgccgct ctccgcggtc
     7321 acgggacgcg ccgagatcat ggacgcgccg cacatcagcg ggcttggcgg cacctacggc
     7381 ggcaacccga tcgcgtgcgc ggcggcgctg gccacgatcg agaccatcga ggcggacggg
     7441 ctggtggcgc gggcggcgga catcgagaac gtgatgacgg acgcgctcgg ccggatgcag
     7501 gccgaggacg accggatcgg cgacgtccgg ggccgaggcg cgatgatcgc gatggaactg
     7561 gtcaagcccg gcaccgccga gccggacccg gtgctgaccc gcacgctgtc cacgcgggcg
     7621 cacgcggccg gcgtgatcgt gctgacgtgc ggcacgttca acaacgtcgt gcggttcctg
     7681 ccgccgctgc cgatcagcga cgagctgctt gtcgagggcc tcgacgtgct ggcttcgatc
     7741 ctcgccgaga ctgcaaatcc gcggtgaaag tacgagtaac cgcctgccgg aatacagttt
     7801 cggcgctggg aaaggaacat tcatgacgac ggtgcagaac ttcatcgacg gcaagcttgt
     7861 gaattcggtg agcggcgcga cgatgccact ggtcgacccg gcgaccggag agaagtacgg
     7921 caccgcaccg gtttccaacg aggaggacat cgacaacgcg tatgccgctg cggcgcgggc
     7981 gttttcggat tggaaacgca ccacgccggc acatcggcag aaggcgctgc tcgacttcgc
     8041 cgacgaggtg gagcaggccg cccgggcgct tgtcgaggcc gagggccgca acaccggcaa
     8101 gcccaaccac gtcaccatgg ccgaggaaat tcctccgatg gtcgaccaga tcaggttctt
     8161 cgccggtgcc gcaagaattt tggagggcaa gtccgcgggc gaatacatgg agggccacac
     8221 gtcgtggata cgccgcgagc ccgtcggcgt catcggtcag gtgacgccgt ggaactaccc
     8281 gatgatgatg gcgatctgga aattcgcgcc ggccatcgcg gcgggcaaca cggtcgtcat
     8341 caagcccagc gacaccactc cggtgaccac cgtgatgctc gccgagatgg cgtccaagca
     8401 cctgccgccg ggggtgctca acgtggtcac cggagaccgg gtgaccggcg ccgcgctcgt
     8461 cgcgcacccg acaccgcaga tggtgtcgat caccggctcg gtggccgcgg gccgcgcagt
     8521 cgccgtatca gccggcggca acctgaagcg cacgcatctg gaactcggcg gcaaggcgcc
     8581 ggtgatcgtg ttccccgacg ccgacatcgc cgccgccgcc gagggcatcg cgacggccgg
     8641 ctacttcaac gccgggcagg actgcaccgc ggccacccgg gtgatcgggc acgcggccgt
     8701 ggcaaacgac ctgaccgacg cgctggccga gcaagcccga agcgcggcaa cgacattcgg
     8761 tagggcggcc gacgacgagg acgcctgggt gccgccggtg aacaacgcca accagctgca
     8821 gcgggtgctc ggcttcctgg ccgaggtgcc cgtccacgcg acggtcgcgg tcggaggtgg
     8881 ccggcagggc gagcgcggct actacgtcga gccgacggtc atcggcgggc tgggccaaga
     8941 cgaccggctg agccagaacg agatcttcgg gcccgtgatc acggtgcagt cgttcgacga
     9001 cgaagacgac gtggtgcgac tggccaacgg cgtcgagtac gggctggcgt cgtcggtgtg
     9061 gaccaaggac gtgtcggtgg cgctgcgggt gtccgcggca ctggacttcg gctgcgtctg
     9121 gatcaacacc cacattccgc tggtcgccga aatgccgcac ggcggtttca agtcttcggg
     9181 acatggcaaa gacctctcca tgtatgggtt cgaggactac acccgcgtca agcacgtgat
     9241 ggcgcggatt tcctagcggc cgttgggact aaatcccctg cgaagggggc cagcggactc
     9301 ttcgaatccg gggccaacct aactattctc gtaaccagaa agagcctgca gggggcgcca
     9361 atgaggatcg tggttcccgt aaacgatgcg cgagaaccgg caattacggc gctctgccca
     9421 aacccttacc gcgcaccgcg gcatgcctac gccaatcacc gacctgacgg cttggccgcc
     9481 gcgcgccact agtcagggct gagagggatc acgcgatgac gacagcagtt ggcctggatc
     9541 gggaaaccct cgagatgatg ctgtcctcgt tggacgagtt cgtggccgag acactgccgg
     9601 accagcgccg cctagacctc gaccatgagg acgtctgccc ggaggacacg gtccgcgaga
     9661 tgtgcagcga ggaactcggt gtgcagctgg tgttcatccc cgaggagtac ggcgggctgg
     9721 gcggcggggc gtttgactcg taccgggtct gcgagcggct tgcccgcgtc gacatcgggg
     9781 tggcgacgtc ggtgttcgcc accttcctcg gcagcgaccc catcctggtc ggcgcaaccg
     9841 acgagcagcg caaggaatgg ctgggccgca tcgccgagca gggcatcatc ttcgcctacg
     9901 gcgcgaccga gcccgaagcc ggcagcgacc tgggcgcgat gacgaccacc gcgcacccga
     9961 tcgagaccga cggagaagtc accgcctacc ggatcaacgg ccgcaagcag tggatcagca
    10021 acggctcgat cgccgacgtc tactcgatcc tggcgctcgc ccccggcggc ccatcgtggt
    10081 tcgtcgtgga aaagggcacc cccggcttct cgacggcgcc gccggaagac aagcacggga
    10141 tccggctctc caataccgcc gcgctgttcc tcgacgacgt cgtcgtgccc gcggagaacc
    10201 tggtgggccg cgaggagggc cgcggcctcg tccaggccca gcaggtgttc ggctacaccc
    10261 gcgtgatggt cggcgcgttc gggttaggtg gcggctggga ggcgctggac cgcgcgatcg
    10321 cctactccga gaaccgggtg cagggcgggg ccccgctgtc gaccaagcag ggctacaccc
    10381 acaagctgat cgtgccgcac gcggtgcggc tggaagcggc gcgggcgttt ctggaggaga
    10441 ccgcgtcgcg gatcgacgcc ggcgagggcg gcgcaggggc gctgaacacc gaaggcgcga
    10501 tcgccaagct gttcgcgacc gagtccggca acgccgccgc cgaggcggcg atccaggcgc
    10561 acggcggcta cgggtacacc cgtccgtacc tcgtcgagaa gatcaagcgt gacgtccgga
    10621 tcaccacgat ctacgagggc acgtcggaaa tcctcgagat gaccatcgcc cgagaccgct
    10681 ggcagcagca cctcaagacc tccggcgcct actaccggga cgcggcccgc gcactcgacg
    10741 gtgtccccga cgagtacggc ggccccacgg cggccctggc gttggagtgc ctggcgtcgg
    10801 tcctcgaggc ctgccgagtc ggccggctga cccgcaacca gcacgtcctg ctgcggctgg
    10861 gcgaactggt cacctacgcc gaatgc
//
LOCUS       DASWBF010000498         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_712273,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000498 DASWBF010000000
VERSION     DASWBF010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712273"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VF874_18270"
     CDS             <1..925
                     /locus_tag="VF874_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX9177930.1"
                     /translation="DGFIEDRRATDRGLINQGWKDSFDGINDATGHTADPPIALCEVQ
                     GYHYAALLARAELADAFGEPGTAARLRERADVLQARFLDAFWLPEQGWYAIALDGRKR
                     PIDALTSNVGHCLWTGIATDEHAEVIVNRLSREEMDSGFGLRTLATTMGAYNPMSYHN
                     GSVWPHDTAIAVAGQLRYRHVPGAVPLAERLAIGLLDAGDAFGGRLPELFCGFPRSQF
                     ACPVPYPTSCSPQAWASAAPLLLVRSFLGLHPHVPHRRLVVSPQLPRAWGRVALTDLR
                     LGDATVHVEAEGQAAKVGGLPDDWQLITPQE"
     gene            1036..1479
                     /locus_tag="VF874_18275"
     CDS             1036..1479
                     /locus_tag="VF874_18275"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177931.1"
                     /translation="MATRGFVVNAGTPVALAKWTRRWASRRFAIKGANGLGRGIAQVL
                     VTADKDVVDVPSTLAMKVRVLSTGGGRKTDPDDAYAVALCALHHKGLHAVHRKDQTTI
                     LRLLSERRPPVEPRSDCPPTQRLFVIQSALAALWSAPLWSSQRSR"
     gene            complement(1449..1576)
                     /locus_tag="VF874_18280"
                     /pseudo
     CDS             complement(1449..1576)
                     /locus_tag="VF874_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_069599718.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          245 a          517 c          542 g          272 t
ORIGIN      
        1 cgacgggttt atcgaagacc ggcgcgccac cgaccgtggg ctgatcaacc agggctggaa
       61 agacagcttc gacggcatca acgacgccac cggtcacacc gccgatccgc cgatcgcgct
      121 ctgcgaggtg cagggttacc actacgcggc cctgctggcc cgcgccgaac tggccgacgc
      181 gttcggcgaa cccgggacgg cggcgcggct gcgcgagcgc gccgacgtgt tgcaggcgcg
      241 gttcctcgac gcgttctggc tgcccgagca gggctggtat gcgatcgcgc tggacggtcg
      301 caaacgcccc atcgacgcgt tgaccagcaa cgtcggccac tgcctgtgga cggggatcgc
      361 gaccgacgag cacgccgagg tgattgtcaa ccggctgtcc cgcgaggaga tggactccgg
      421 attcggccta cgcacactgg cgacgacgat gggcgcctac aacccgatga gctaccacaa
      481 cggctcggtc tggccgcatg acacggcgat cgcggtcgcg ggtcagttgc gctatcggca
      541 cgtcccggga gcggtgccgc tcgccgaacg actcgcgatc ggattgctcg acgccggcga
      601 cgccttcgga ggccggttac cggagctttt ctgcggcttc ccgcgatctc agttcgcttg
      661 tccggtgccc tatcccacgt catgttcgcc gcaggcgtgg gccagcgctg cgccgctgct
      721 gctggtgcgc tccttcctcg ggctgcaccc gcatgtgccg caccgccggc tcgtcgtgtc
      781 cccgcagctg ccgcgggcct ggggccgtgt cgcgctgacc gatctgcggc tgggtgacgc
      841 gacggtacac gtcgaggcgg agggccaggc ggcgaaggtg ggtggtctgc ccgacgactg
      901 gcagctgatc accccgcagg agtgagccct gcgagtcgat atggtggcac catgattggt
      961 gtcacccgtg acggccatgt cacacccttg gaactgcggg ctaaccgccg tcgcgattga
     1021 caatctggca ccaacttggc cacccgcggg ttcgtcgtca acgccgggac tccagtagcg
     1081 ttggcgaaat ggacccgacg ttgggcgtcc cgccgtttcg cgatcaaggg agccaacggc
     1141 cttggtcgcg ggatcgccca ggttctcgtc acagccgaca aggatgtcgt ggacgtgccc
     1201 tcgacactcg cgatgaaggt gcgggttctc agtaccggag gaggccgcaa gaccgatccc
     1261 gacgacgcct acgcggtagc gctttgcgcg ttgcaccaca aaggattaca cgcggtgcat
     1321 cgcaaagacc agacgacaat tctgcggctg ttgtccgagc gtcgaccccc ggtggagccc
     1381 cgctcggatt gtccgccgac acagcggctc ttcgtaattc agagtgcatt ggcggctttg
     1441 tggagtgcgc cgctgtggag cagccagcgc agtcggtagt gggtgaggtt tctgaatccg
     1501 agggcgttgc ggcgcagtgc ttccagacgg ccgttgatcg cttcggtggg gtgctgccgc
     1561 tgctgacctg cacggg
//
LOCUS       DASWBF010000499         9018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_115051,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000499 DASWBF010000000
VERSION     DASWBF010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115051"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VF874_18285"
     CDS             <1..720
                     /locus_tag="VF874_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9177932.1"
                     /translation="GWCAKSGWKVDGRPDWAAKLPEELLEAQAETIVATPVFDGAREE
                     ELQGLLSSVQPNRDGDVLVNADGKAVLYDGRSGEPFPYPVTVGYMYILKLHHLVDDKI
                     HARSTGPYSMITQQPLGGKAQFGGQRFGEMECWAMQAYGAAYTLQELLTIKSDDTVGR
                     VKVYEAIVKGENIPEPGIPESFKVLLKELQSLCLNVEVLSSDGAAIEMRDGDDEDLER
                     AAANLGINLSRNESASVEDLA"
     gene            767..4723
                     /locus_tag="VF874_18290"
     CDS             767..4723
                     /locus_tag="VF874_18290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX9177933.1"
                     /translation="MLDVNFFDELRIGLATAEDIRNWSFGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTRDWECYCGKYKRVRFKGIICERCGVEVTRAKVRRERMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDLAPKDLEKIIYFAAYVITAVDDEMRHNELSTLEAEMEVEKK
                     ATEDQRDADLEARAQKLEADLKELEEEGAKADVRRKVRDGGEREMRQIRDRAQRELAR
                     LEEIWTTFTKLAPKQLIVDEVLYRELVDRFGEYFTGSMGAEAIQKLIETFDIDAEADL
                     LREVIRNGKGQKKLRALKRLKVVAAFQQSGNSPMGMVLDAVPVIPPELRPMVQLDGGR
                     FATSDLNDLYRRVINRNNRLKRLIDLGAPEIIVNNEKRMLQESVDALFDNGRRGRPVT
                     GPGNRPLKSLSDLLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALE
                     LFKPFVMKRLVDLNHAQNIKSAKRMVERQRPQVWDVLEEVIGEHPVLLNRAPTLHRLG
                     IQAFEPMLVEGKAIQLHPLVCEAFNADFDGDQMAVHLPLSAEAQAEARILMLSSNNIL
                     SPASGKPLAMPRLDMVTGLYYLTSEVPGDVGEYQPAAKDRAESGVYSSPAEAIMAMDR
                     GVLSVRARIKVRLTQLRPPAELEAELFGGEGWKPGDAWTVETTLGRVLFNELLPHGYA
                     FINEQMHKKVQARIINDLAERYPMIVVAQTVDKLKDAGFYWATRSGVTVSMADVLVPP
                     EKGEILERYEKEADGIEKKYQRGALNHQERNEALVKIWQDATEEVGEALRSHYPDDNP
                     IITIVESGATGNFTQTRTLAGMKGLVTNPKGEFIPRPIKSSFREGLTVLEYFINTHGA
                     RKGLADTALRTADSGYLTRRLVDVSQDVIVREHDCGTERGITVRLAEVQPDGSLAKDQ
                     YVETSAYARTLAEDATDEKGKVLVEKGHDLGDPAIDLLLENGISSVRVRSVLTCASAN
                     GVCAMCYGRSMATGKLVDIGEAVGIVAAQSIGEPGTQLTMRTFHQGGVTGGQDITGGL
                     PRVQELFEARVPRDKAPIAEVSGRIRIEEGEKFYKITIVPDDGSEEVVYDKLSKRGRL
                     RAVTHEDGTEGLLRGGDHVEVGNQLMEGSADPHEVLRVQGPREVQLHLTHEVQEVYRA
                     QGVSIHDKHIEVIVRQMLRRVTIIDSGSTEFLPGSLTERSEFEAENRRVVAEGGEPAA
                     GRPVLMGITKASLATDSWLSAASFQETTRVLTDAAINCRSDKLNGLKENVIIGKLIPA
                     GTGINRYRNIQVQPTEEARAAAYTIPSYEDQYYSPDFGQATGAAVPLDDYGYSDYR"
     gene            complement(4724..5569)
                     /locus_tag="VF874_18295"
     CDS             complement(4724..5569)
                     /locus_tag="VF874_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9177934.1"
                     /translation="MSGVRLDERRSRVRDLVDTRPLTARSVLASALLGADEPSLPVAD
                     LIVIASMFGISPGAARTCLWRMVSNGELTTDDASYTLAGRLLERRDRVDDAARREDPT
                     TGPWDGTWELAIVSQQRRSAADRLELRKAATALHLAEIREGVWIRPDNLDPERLPPSR
                     AVLDRQCVHFHRAETDVPVDIARSLFPLDTWARDARRLTRAMDGERQTADFAYQFALS
                     IAVVRHLQLDPLLPDVLNPKDWPAKRLRSTYLRFDRAFKRGMNEEFTRHSGATAPLTA
                     VRARR"
     gene            5619..6755
                     /locus_tag="VF874_18300"
     CDS             5619..6755
                     /locus_tag="VF874_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9177935.1"
                     /translation="MATTAQTMLAEDVEIVHRVLAHIDAGTTDTGDVWREPVENYVAS
                     ARYAAELRMLRSTPSVFVPSAAILNAGDYVERVAFGVPIFAVRGRDRRVRVFRNSCRH
                     RGMSLVEGRGCSQALVCRYHGWTYRLDGSLAHVPHADGFPDLHMPSRGLVEVASREIG
                     GLVVIDGLDSHATTAVMAALTDGSPWRDKLPAARRLVFAESTVRAINWKVLVEQFLEG
                     YHIRSTHRDTFFPLQYDNLNIVERFGPNSRITFPYRNIERLRNRPESTWTVDRRVTFV
                     YHLFPNVMLATFPNQVIMVVIDPIDIDHSATTTYAMADQVPDRDGAPSLIDRGALEDN
                     AMSAAVQRGLHAGANPFVEFGRHESAIGHFHANLEERLGALPNG"
     gene            6790..7638
                     /locus_tag="VF874_18305"
     CDS             6790..7638
                     /locus_tag="VF874_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX9177936.1"
                     /translation="MRLLALLAALLAVAGCSSSPRGTAEPKDEFPEGTSVHTISVGGL
                     ERSYRLHKPAGLPASASLVVVLHGGFGSGEQAERAYGWDELAESAKFVVAYPDGIARA
                     WNVHGCCGRPSRDGIDDVGFITAMVGDVSRGIGIDQPRIYATGISNGGMMSYTLACSS
                     DMFAAIGPDSATQLDPCASPRPTSVMHIHGTADRLIRYDGEEGEGVAHVNGPPVPEVN
                     AFWRNVNQCGEPVVTTDGPVTTSAADCPGNRSVVLVTVDGGRHEWPAFATQRLWEFFA
                     AHPRAA"
     gene            7924..>9018
                     /locus_tag="VF874_18310"
     CDS             7924..>9018
                     /locus_tag="VF874_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177937.1"
                     /translation="MPDTHTVTNQVPPLENYNPATSPVLVEALIREGGQWGLDEVTEL
                     GALSGSQQAQRWGELADRHRPILRTHDKHGHRIDEVEYDPAYHELMRVAIGHGLHGAP
                     WADDRPGAHVVRAAKTSVWTPEPGHICPISMTYAVVPALRVDAELAETYEPLLTSREY
                     DPELKAPTIKAGITAGMSMTEKQGGSDVRAGTTQATPNADGSYRLTGHKWFTSAPMCD
                     VFLVLAQAPGGLSCFFLPRILPDGTRNRMLLQRLKDKLGNHANASSEVEYDGATAWLV
                     GEEGRGVPTIIEMVNLTRLDCTLGSATSMRNGLTRAIHHAQHRKAFGAYLIDQPLMRN
                     VLADLAVEAEAATIVAMRMAGATDNASRGDE"
BASE COUNT         1646 a         2898 c         3056 g         1418 t
ORIGIN      
        1 ggttggtgcg cgaagagcgg ctggaaggtg gacggtaggc ctgactgggc ggcaaagctg
       61 cccgaggagc tgctggaagc gcaggccgag accatcgtgg ccacgccggt gttcgacggc
      121 gcccgcgagg aggagctgca ggggctgctg tcctcggtgc agccgaaccg cgacggcgac
      181 gtactcgtca atgccgacgg caaggcggtg ctctacgacg gccgcagcgg cgaaccgttc
      241 ccgtacccgg tgacggtggg ctacatgtac atcctgaagc tgcaccacct ggtggacgac
      301 aagatccacg cccggtccac cggcccgtac tcgatgatca cccagcagcc gctgggcggt
      361 aaggcgcagt tcggcggcca gcggttcggc gagatggagt gctgggccat gcaggcctac
      421 ggcgcggcgt acacgctgca ggagctgctg accatcaagt ccgacgacac cgtcggccgg
      481 gtcaaggtgt acgaggcgat cgtcaagggc gagaacatcc ccgagccggg catccccgag
      541 tcgttcaagg tgctgctcaa ggaactgcag tcgctgtgcc tcaacgtcga ggtgctgtct
      601 agcgacggtg ccgcgatcga gatgcgcgac ggcgacgacg aagacctgga gcgcgctgcc
      661 gcgaacctgg gaatcaactt gtcccgcaac gaatctgctt ctgtagaaga cctagcgtga
      721 tttggcgatt gtcattttct agtcccgaaa ggggaaaggg agttacgtgc tagacgtcaa
      781 cttcttcgat gaactccgga tcggcctggc gaccgccgag gacatccgga attggtcctt
      841 cggcgaggtc aagaagccgg agaccatcaa ctaccgcacg ctgaagccgg agaaggacgg
      901 cctgttctgc gagaagatct tcggaccgac tcgcgactgg gagtgctact gcggcaagta
      961 caagcgcgtg cgcttcaagg gcatcatctg cgagcgctgc ggcgtcgagg tgacccgcgc
     1021 caaggtgcgc cgtgagcgga tgggtcacat cgaactggcc gcgcccgtca cgcacatctg
     1081 gtacttcaag ggcgtcccgt cccggctggg ctacctgctc gacctcgcgc cgaaggacct
     1141 cgagaagatc atctacttcg ccgcgtatgt gatcacggcc gtcgacgacg agatgcgcca
     1201 caacgagctg tccactctcg aagccgagat ggaggtcgag aagaaggcca ccgaggatca
     1261 gcgtgacgcc gacctggagg cccgcgccca gaagctcgag gccgatctca aggagctgga
     1321 agaggaaggc gcgaaagccg acgtgcggcg caaggtccgc gacggcggcg aacgcgagat
     1381 gcgtcagatc cgcgaccgcg cgcagcgaga gctggcccgg ctcgaggaga tctggacgac
     1441 gttcaccaag ctggcgccca agcagctgat cgtcgacgag gtgctctacc gcgagctcgt
     1501 cgaccgcttc ggcgagtact tcaccggctc gatgggtgcg gaggcgatcc agaagctcat
     1561 cgagaccttc gacatcgacg ccgaggccga cctgctgcgt gaggtcatcc gaaacggcaa
     1621 ggggcagaag aagcttcgcg ccctcaagcg gctaaaggtg gtcgcggcgt tccagcagtc
     1681 gggcaactcg ccgatgggta tggtgctcga cgcggtgccg gtgattccac cggaactgcg
     1741 cccgatggtg cagctcgacg gcggccgctt tgcgacctcc gacctgaacg acctgtaccg
     1801 gcgcgtgatc aaccgcaaca accggctgaa gaggctgatc gacctcggcg cgcccgagat
     1861 catcgtcaac aacgagaagc ggatgctgca ggagtcggtg gacgcgctgt tcgacaacgg
     1921 ccgccgcggc cggcccgtca ccgggccggg caaccgtccg ctgaagtcgc tgtcggatct
     1981 gttgaagggc aagcagggcc ggttccgtca gaacctgctc ggcaagcgcg tcgactactc
     2041 gggccgttcg gtgatcgtcg tcggcccgca actcaagctg catcagtgcg gtctgcccaa
     2101 gctgatggca ctcgagctgt tcaagccgtt cgtgatgaag cggctggtcg acctgaacca
     2161 cgcgcagaac atcaagagcg ccaagcggat ggtcgagcgt cagcgtccgc aggtgtggga
     2221 cgtgctcgaa gaggtcatcg gcgagcaccc ggtactgctg aaccgggcgc ccacgctgca
     2281 ccggctcggc atccaggcgt tcgagccgat gctggtggaa ggcaaggcga ttcagcttca
     2341 cccgctggtg tgcgaggcct tcaatgccga cttcgacggc gaccagatgg ccgtgcacct
     2401 gccattgagt gccgaggcgc aggccgaggc gcgcatcctg atgctgtcga gcaacaacat
     2461 cctgtcgccg gcgtcgggca agccgctggc catgccgcgt ctggacatgg tcaccggcct
     2521 gtactacctg accagcgagg ttcccggcga tgtcggcgaa taccagccgg ccgccaagga
     2581 ccgcgcggag tcgggcgtct acagctcacc ggccgaggcg atcatggcga tggaccgcgg
     2641 tgtgctttcg gtgcgcgcgc ggatcaaggt acggctgacg cagctgcggc cgccggccga
     2701 actcgaagcc gagctgttcg gtggcgaggg ctggaagccg ggcgacgcgt ggaccgtcga
     2761 gaccacgctg ggccgggtgc tgttcaacga gcttctgccg cacggatatg cgttcatcaa
     2821 cgagcagatg cacaagaagg tgcaggcccg gatcatcaac gacctggccg agcgctaccc
     2881 gatgatcgtg gtggcgcaga ccgtcgacaa gctcaaggac gccggcttct actgggccac
     2941 ccgatcgggt gtcaccgtgt cgatggccga cgtgctggtg ccgccggaga agggcgagat
     3001 cctcgagcgc tacgagaagg aagccgacgg gatcgagaag aagtatcagc gcggcgccct
     3061 gaaccatcag gagcgcaacg aggcactggt caagatctgg caggacgcca ccgaagaggt
     3121 cggcgaggcg ctgcggtcgc actaccccga cgacaacccg atcatcacga tcgtcgagtc
     3181 gggcgcgacc ggtaacttca cccagacccg gacgctggcc ggcatgaagg gcttggtgac
     3241 caacccgaag ggtgagttca tcccgcggcc catcaagtcg tcgttccgcg agggcctgac
     3301 ggtgctggag tacttcatca acacccacgg cgcccgaaag ggcttggccg acactgcgtt
     3361 gcgtaccgcc gactcgggtt acctgacccg tcgtctggtg gacgtgtcgc aggacgtgat
     3421 cgttcgcgag cacgactgcg gcaccgagcg cggcatcacg gtccggctcg ccgaggtgca
     3481 gcccgacggc agcctggcca aggaccagta cgtcgaaaca tcggcgtacg caaggacttt
     3541 ggccgaggac gcgaccgacg agaagggcaa ggtgctcgtc gagaagggcc atgatctcgg
     3601 tgacccggcg atcgacttgc tgctggagaa cggcatctcg tcggtgcggg tgcggtcggt
     3661 gctgacatgc gcgagcgcca acggcgtctg tgcgatgtgc tacggccggt cgatggcgac
     3721 gggcaagctc gtggacatcg gcgaggcggt tggcatcgtc gccgcgcagt cgatcggcga
     3781 gcccggcacg cagctgacca tgcgcacgtt ccaccagggc ggtgtcaccg gcggccagga
     3841 catcacgggt ggtctgcccc gcgtgcagga gctgttcgag gcccgcgtgc cgcgcgacaa
     3901 ggcgcccatc gccgaggtgt ccgggcggat ccggatcgaa gagggtgaga agttctacaa
     3961 gatcaccatc gttccggacg acggcagcga ggaagtcgtg tacgacaagc tgtccaagcg
     4021 cgggcggctg cgtgcggtca ctcacgagga cggcacggag ggcctgctgc gcggcggtga
     4081 ccatgtcgag gtcggcaacc agctgatgga aggctccgcg gacccgcacg aggtgctgcg
     4141 cgtgcagggc ccgcgcgagg tgcagctgca cctgacccac gaggtccagg aggtgtaccg
     4201 agcgcagggc gtgtcgattc acgacaagca catcgaggtg atcgtccggc agatgctgcg
     4261 gcgcgtgacc atcatcgact cgggcagcac ggagttcctg cccggctcgc tgaccgagcg
     4321 ctccgagttc gaggccgaga accgtcgggt ggtggccgag ggcggcgagc ccgccgccgg
     4381 ccgcccggtg ctgatgggca tcacgaaggc gtcgctggcc accgattcgt ggctctcggc
     4441 ggcgtcgttc caggagacca cgcgagtgct gaccgatgcg gcgatcaact gccgcagcga
     4501 caagctcaat ggtctgaagg agaacgtgat catcggcaag ctgatcccgg ccggtaccgg
     4561 aatcaaccgg taccgcaaca tccaggtgca gccgaccgaa gaggcgcggg ccgctgcgta
     4621 cacgatcccg agctacgagg atcagtacta cagccccgac ttcggccagg cgaccggtgc
     4681 cgcggttccg ctggacgact acggctacag cgactaccgc tagtcacctt ctggcgcgaa
     4741 cagctgtcag gggagctgta gctccgctgt ggcgggtgaa ttcctcgttc atccctcgct
     4801 tgaaagcacg gtcgaagcgg aggtaggtgc tgcgcagtct tttcgcgggc cagtccttcg
     4861 ggttgaggac atctgggagc agcgggtcga gttggagatg gcgcaccact gcgatggaga
     4921 gcgcgaattg gtaagcgaag tccgcggttt ggcgctcgcc gtccatggcg cgagtaagtc
     4981 gtcgcgcgtc acgcgcccac gtgtcgaggg ggaacagtga ccgcgcgatg tcgacgggga
     5041 cgtctgtctc ggcgcggtga aagtgcacac actgccggtc gaggacggcg cgtgatggtg
     5101 gcagtcgctc agggtcgagg ttgtcggggc ggatccacac accttccctg atctcggcaa
     5161 gatgtagcgc cgtggccgcc ttgcggagtt cgagtctgtc agccgcgctg cggcgttgtt
     5221 gtgacacgat cgccagctcc cacgtcccgt cccacgggcc ggtggtcggg tcctcgcgtc
     5281 gggcggcgtc gtcgacgcgg tcgcgtcgtt cgagcaggcg cccggctagc gtgtagctgg
     5341 catcgtcggt ggtgagttcc ccgttggaca ccatccgcca caggcatgtg cgcgcagcgc
     5401 cgggactgat accgaacatc gacgcgatga cgatcagatc cgcgaccgga agcgacggct
     5461 cgtctgcacc cagcaatgcc gaggcgagta ccgagcgggc ggtcagcgga cgcgtgtcga
     5521 ccaggtcgcg cactcgggac cgtcgctcgt ccagccggac tccactcata tcacataatc
     5581 cttgacacgt gattatggta tcgtgatact tgcacagtat ggcaactact gcccagacga
     5641 tgctcgccga agatgtcgag atcgtccacc gcgtgctcgc gcacatcgat gcgggaacca
     5701 cagataccgg tgacgtctgg cgtgaaccgg tcgagaatta tgtggcttcc gcgcggtacg
     5761 ccgccgagct gcggatgctt cgttcgacac ccagcgtatt cgtcccttcc gcggcgatcc
     5821 tcaacgccgg cgactacgtc gaacgggtcg cattcggtgt gccgatcttc gcggtgcgcg
     5881 gccgcgatcg ccgagttcga gtgtttcgca actcatgtcg ccatcgcggt atgtcactag
     5941 tcgagggccg ggggtgttct caggctttgg tctgccgcta ccacggttgg acctaccgcc
     6001 tcgacggatc actcgcacac gtcccgcacg ccgatgggtt cccggatctc cacatgccgt
     6061 ctcgcggtct cgtcgaggtc gccagccgcg agatcggcgg tctggtcgtc atcgatggcc
     6121 tggactcgca tgccacaacc gcggtcatgg cggcactcac cgacggcagc ccgtggcggg
     6181 ataagctgcc agccgccaga cgcctcgtct tcgcggaatc aacggtgcgg gcgattaatt
     6241 ggaaggtgct cgtcgagcag tttctggagg ggtaccacat acgctcgacg caccgtgaca
     6301 cgttcttccc cttgcagtac gacaacctca acatcgtgga gaggttcggc ccgaacagtc
     6361 gaatcacctt cccctaccgc aacatcgagc ggctccgcaa ccgaccggaa tcgacctgga
     6421 ctgtggaccg acgcgtcaca ttcgtctacc acctgttccc gaacgtgatg ctggcgacgt
     6481 tccccaacca agtcatcatg gtcgttatcg acccgatcga catcgaccat tcggcaacaa
     6541 ccacttatgc catggccgac caagtgcccg accgcgacgg cgcacccagt ctgatcgacc
     6601 ggggtgcgct cgaggacaac gcgatgtcgg cagccgtgca gcgggggcta catgccggcg
     6661 ccaacccgtt cgtcgagttc ggccggcatg agagcgcgat cggacacttc cacgccaacc
     6721 tcgaggagcg tctcggggca ttgccaaacg ggtgaacgca tcgccgccgt tagtccgtac
     6781 ggtggtgata tgcgactgct cgccctgctg gccgcgctgt tggcggtcgc gggctgctcg
     6841 tcgtcccccc gcggcacggc ggagcccaag gacgaattcc ccgagggcac cagcgtccac
     6901 acgatcagcg tcggcgggtt ggagcggtcc tatcggctgc acaagccagc gggcctgccg
     6961 gcgtcggcgt ctctggtggt ggtgttgcac ggtggcttcg gcagcggaga gcaggccgaa
     7021 agagcttacg gctgggatga attggcggaa agcgcaaagt tcgtcgtggc ctatcccgac
     7081 ggcatcgctc gggcgtggaa tgtgcacggc tgctgcggtc gacccagccg cgacggtatc
     7141 gacgacgtcg ggttcatcac ggcaatggtc ggggatgtga gccgaggcat cggcatcgac
     7201 cagccacgga tctacgcgac cggcatcagc aacggcggca tgatgagcta cacgctggcc
     7261 tgcagcagcg acatgttcgc cgcgatcggc cctgactccg ccacacagct ggacccgtgc
     7321 gcttccccgc gccccacgtc ggtgatgcac atccacggca ctgccgaccg cctgatccgc
     7381 tacgacgggg aagaaggcga aggggtcgcc cacgtcaacg gcccgcccgt gcccgaggtg
     7441 aacgcgttct ggcgcaacgt caaccagtgc ggcgagcccg tcgtcaccac cgacggaccg
     7501 gtcaccacct cagccgccga ctgccccggc aaccgcagcg tcgtgctggt cacggtcgac
     7561 ggcggcaggc acgagtggcc ggcgtttgcg acgcaacggc tgtgggagtt tttcgccgcc
     7621 cacccccgcg ccgcctagag tcacagcaat ctcttgcgtc accggccggg gcggtaatcg
     7681 aagtgcccac cagtgagcga cacccgctga cctgcgttga agccgcgggt tgtcgctgag
     7741 cgctctgctc tcgtcggggc ttggacccgg gaaaacgcga ttgtcgctgc gaggcgggca
     7801 aatgcatcgc ggtccgcccg acaggcgggt ggggcaggtg aggcagatgg gacggcgcac
     7861 caatgccgcc tacgagtgtt acgcatcttg tgcggctgtc gtgagaatgt aacactgcgt
     7921 ggtatgcctg acacgcacac cgtcaccaac caggttccgc cgctggagaa ctacaacccc
     7981 gccacctcgc cggtgctcgt cgaggcgctg atccgcgagg gcgggcagtg ggggctcgac
     8041 gaggtcaccg agctcggcgc gctctccgga tcacagcaag cccagcggtg gggcgagctc
     8101 gccgatcggc atcgcccgat cttgcgcacc cacgacaagc acggccatcg catcgacgag
     8161 gtcgagtacg acccggcgta ccacgagctg atgcgcgttg cgatcggcca cggcctgcac
     8221 ggggcgccgt gggccgacga ccggcccggc gcccacgtcg tccgcgcggc gaagacgtcg
     8281 gtgtggacgc cggaacctgg gcacatctgc ccgatctcga tgacgtacgc cgtcgtcccg
     8341 gcactgcgcg tcgatgcgga gctcgccgaa acctacgagc cactgctcac cagccgcgaa
     8401 tacgacccgg agctgaaagc cccgaccatc aaagccggta ttaccgcggg catgtccatg
     8461 accgagaagc agggcggctc cgacgtgcgc gccgggacta cccaggcgac acccaacgcc
     8521 gacgggagtt accgcctcac cgggcacaag tggttcacgt cggcgccgat gtgcgacgtg
     8581 ttcctggtgc tcgcgcaggc acccggtggg ctgagctgtt tcttcctgcc gcgaatcctg
     8641 cccgacggga cccgcaaccg aatgttgttg cagcggttga aggacaagct cggcaaccac
     8701 gcgaacgcct ccagcgaggt cgagtacgac ggcgccaccg cctggctagt cggcgaagag
     8761 ggccgcggcg tgccgacgat catcgagatg gtcaacctca ccaggctcga ctgcacactg
     8821 ggcagcgcca ccagcatgcg caacggcctc acccgggcca tccaccacgc tcagcatcga
     8881 aaggcgttcg gcgcctacct gattgaccag ccgctgatgc gcaacgtact cgccgacctc
     8941 gcggtcgagg ccgaggcggc gacgatcgtc gcgatgcgga tggccggtgc caccgacaac
     9001 gcgtcacgcg gcgacgaa
//
LOCUS       DASWBF010000500        12050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1165131,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000500 DASWBF010000000
VERSION     DASWBF010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12050
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165131"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VF874_18315"
     CDS             complement(<1..488)
                     /locus_tag="VF874_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9177938.1"
                     /translation="MAGKARVHELAKELGVTSKEVLARLSEQGEFVKSASSTVEAPVA
                     RRLRESFGGGKAPQAAAQDGNGAAAKSSSVATAPAEAVPAKAAAAAAPAPPAAAAPPA
                     APAPATAQPSTPAASAPAPSPARPAPTPGGPRPGPAPGPRPAPRTPRVGNNPFSTVQP
                     VDR"
     gene            complement(554..934)
                     /locus_tag="VF874_18320"
     CDS             complement(554..934)
                     /locus_tag="VF874_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX9177939.1"
                     /translation="MIQRETSASGAASAATSASDLGPVRTCIGCRRRELAVELLRVVA
                     VPTGNGEYAVTVDTAGNLPGRGAWLHPDQQCLGAAIRRRAFGRALRITGPPDTSEALE
                     HITALEAPGNRTGSEEHEHTVKSR"
     gene            complement(1020..1421)
                     /locus_tag="VF874_18325"
     CDS             complement(1020..1421)
                     /locus_tag="VF874_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9177940.1"
                     /translation="MLIVDASCLFEVVADTPRSGEIAARLAADVDHAAPHVIDVEVLG
                     VIRAQYLRGRMDGTAAGQAVADLRDWPGERFGHRWLLDRAWQLRASVRGWDALYVALA
                     EAFDATLLTLDARLAHAHGPTCRIEVLSEPE"
     gene            complement(1408..1653)
                     /locus_tag="VF874_18330"
     CDS             complement(1408..1653)
                     /locus_tag="VF874_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177941.1"
                     /translation="MPKTVQIRDIDDQVYAALVRRAGEEGITVPELLRREAARLAARP
                     SVVQWLARTGRRPSSVSTAEVLTTLDEWRGEWPDADR"
     gene            complement(1696..2706)
                     /gene="nusA"
                     /locus_tag="VF874_18335"
     CDS             complement(1696..2706)
                     /gene="nusA"
                     /locus_tag="VF874_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX9177942.1"
                     /translation="MNIDMAALHAIEVDRGMSVGESLEIIKSALLTAYRHTQGHQPEA
                     RIEIDRKTGEVTVIARETDVDGNVISEWDDTPEGFGRVAATTARQVMLQRLRDAENEK
                     TYGEFSAREGDVVAGVIQRDARANARGLVVVRMGSETKGSEGVIPAAEQVPGERYEHG
                     ERLRCYVVGVSRGAREPLITLSRTHPNLVRKLFSLEVPEIADGSVEIAAVAREAGHRS
                     KIAVRSRVPGLNAKGACIGPMGQRVRNVMSELSGEKIDIIDYDEDPARFVANALSPAK
                     VVSVSVIDPNARAARVVVPDFQLSLAIGKEGQNARLAARLTGWRIDIRSDNEATQGQP
                     TA"
     gene            complement(2703..3215)
                     /gene="rimP"
                     /locus_tag="VF874_18340"
     CDS             complement(2703..3215)
                     /gene="rimP"
                     /locus_tag="VF874_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX9177943.1"
                     /translation="MATGLPSQEQVIELLDDEFARAGYEIEEVIVDSKARPPRITVIA
                     DGDDPLDLDTIAALSRRASALLDDLDGAAASSYVLEVSSPGVYRPLTSEKHFRRARGR
                     KAELTLSDGSSVTGRLGDTDDGVLRVVLRDKSGWALRDIPLGDIVKAVVQVEFSPPNP
                     RELELAGVVT"
     gene            3425..3925
                     /locus_tag="VF874_18345"
     CDS             3425..3925
                     /locus_tag="VF874_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX9177944.1"
                     /translation="MPGAAPQISRRRVLVGAAALAALGASACGSSPEPPQPDPLEAQL
                     DLAQRDSQMAHAAATAAGPFYAPPLNVVADERAAHAKALSKEVARAAGETATSSTTSP
                     PTTAAPALPPPSREGVIAALRESADSAAKLAVNLDGYRAGLLGSIAAACTASVTVPLA
                     MKEPAQ"
     gene            3922..4392
                     /locus_tag="VF874_18350"
     CDS             3922..4392
                     /locus_tag="VF874_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9177945.1"
                     /translation="MTSPQPSPSKAPESAGDKALAEAIAAEHATIYGYGFVSAHSLPD
                     LNEMISDSLAEHRARREAAIAMLASREVAAPIAAVGYQLPIPVNNWRDAATLAVRMET
                     DDEAAWRAVLEQAESSQDRAFAVTALTQAAVRAARWRQVLGTWPLTSPFPGGGE"
     gene            complement(4396..6138)
                     /locus_tag="VF874_18355"
     CDS             complement(4396..6138)
                     /locus_tag="VF874_18355"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9177946.1"
                     /translation="MITRMSELFLRTLREDPADAEVPSHKLLIRGGYIRPIGPGLYTW
                     LPPGLRVLRKIESIVREEMNAIGGQEILFPALLPRAPYEATNRWTEYGEAVFRLKDRR
                     GNDYMLGPTHEELFTLTVKGEYSSYKDFPLLLYQIQTKYRDEARPRAGILRGREFVMK
                     DSYSFDVDDAGLKTQYHAHRDAYQRMFDRMRVRYVIVSAVTGAMGGSASEEFLAESPI
                     GEDTFVRCAESGYAANVEAVFTARPNPLPFDDLPEPVVHDTPDTPTISTLVDWANSAL
                     DRTVTAADTLKNVLMKVRPPGGDWELLAVGIPGDREIDDKRLGAALDPADYAMLDDGD
                     FAKYPFLVKGYIGPKALAKNGIRFLVDPRVVDGTSWITGADAPGKHVVGLVAGRDFLP
                     DGTIEAAEVRDGDMSPDGAGPLVSARGIEIGHIFQLGRKYTDAFTVDVLGEDGKPVRL
                     TMGSYGVGVSRLVAVIAEQHHDELGLRWPASVSPFDVHLVIANKDAAARDGAAQLARD
                     LDRLGVDVLLDDRQASPGVKFKDAELLGVPWIVVVGRGWANGIVELRNRFGGETREVP
                     VDSAATQIADTLSG"
     gene            6275..6979
                     /locus_tag="VF874_18360"
     CDS             6275..6979
                     /locus_tag="VF874_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177947.1"
                     /translation="MSTTFTARIKDAARAALVATALTGAALGLAGTAHAELYGNPEEA
                     ADFWQHQDFDNCVLMATADMIGELQGDMISEFEITATASITPSVVHPGTIYIPPIGSL
                     DDPNQGMGTDPIDSLVLLEKYGFSGKVGGDLAEEVDEEFTTETGMDALAQYLADGQKV
                     MAIVNAQLIWGDTEGPFDEPNHALVVTGIDTDEGVVHLNDSGNPDGRDFQIDIETFEQ
                     AWATSGQSIVVSDQTS"
     gene            complement(7041..8534)
                     /locus_tag="VF874_18365"
     CDS             complement(7041..8534)
                     /locus_tag="VF874_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9177948.1"
                     /translation="MFAARRTQRSSRPLASSVVGIAIVAITGMQLMSTLDGTIVVVAL
                     PRMQADLGMSDAAKSWVITAYVLTFGGLLLLGGRVGDAVGHKRAFIAGVGVFTAASLV
                     CGVAFDPAMLIAARAVQGIGAAVAAPTGLALIASTYAVGHARNQAMAVSAGMMGIGSV
                     LGLVLGGAFTVISWRLAFLINVPIGIVIIAIAMTRLRETHRERLRLDATGAVLATLAC
                     TTAVLIFTQGPPLGWINPWIFGAAAATVLLFGGFLLVERTADHPLVPLSLFTDRNRVM
                     TFVSLFLAGGVLLTLTVMIGLYVQVVLGYSALRAGISFIPFAVALGLGNVAAARVAPL
                     IAPRWLIIGGGSFVLGAMLYGSTLNRNIPYFPDLFVPIIVGGFGIGVISVILPLCALA
                     DVGPREIGPVAAITLMVQNLGGPLVLVVIQAVQTSRTLYLGGTTGSVRDMTPAQLTAL
                     DHGYTYSLLWVAAVAVLVGISAFWIGFSARQIAAAQYTREAVEAGEL"
     gene            complement(8638..10194)
                     /locus_tag="VF874_18370"
     CDS             complement(8638..10194)
                     /locus_tag="VF874_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9177949.1"
                     /translation="MTALDDAERRTPLDRANKAALEVSQRTGWSRPAWLPSRRFIAAV
                     VAIGGMQLLATMDSTIAIVALPRIQDELNLSDAGRSWVITAYVLTFGGLMLLGGRLGD
                     TIGRKRTFIAGVALFTIASVLCGLAWDEATLVIARLLQGVGAAIASPTALALIATTFP
                     KGPARNTATAIFAAMTGIGSVMGLIVGGALTEVSWRLAFLVNVPIGLLMLYLAYTTLR
                     ETHREPIKLDATGAILATLGCTAAVFGFSMGPEQGWLSPVTLASGLAAVVFLGAFAIT
                     ERTAENPLVPLDLFRDRNRVATFAAVFLAGGVMFTLTVLIGLYVQDIMGYSALRAGVG
                     FIPFVIALGIGLGVSSHLVSLFPPRMLVMGGGVLVLAAMIYGSTLNGGIPYFPNLVLP
                     ITVGGFGIGMIVVPLTVSAIAGVGFDRIGPVSAIALMAQNLGGPVVLAVVQAVITSRT
                     LYLGGTTGPVKNMNAAQLHALDQGYTYGLLWVAAVAVLVGAAALFIGYTSQQVAHAQE
                     VKDALDAGEL"
     gene            complement(10217..11425)
                     /gene="cobA"
                     /locus_tag="VF874_18375"
     CDS             complement(10217..11425)
                     /gene="cobA"
                     /locus_tag="VF874_18375"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX9177950.1"
                     /translation="MTENPYLVGLRLGGKKVVVVGGGSVAQRRLPLLIASGADVHVIT
                     RSATPAVEAMSGISLELREYRDGDLDGAWYAIAATDEPDINAAVVAEAEARHVFCVRA
                     DIAREGTAVTPASFEYEGLAVGVLAGGEHRRSAAIRSAIHEALQQGIIAAETPEVTHS
                     GVALVGGGPGDPELITVRGRRLLAHADVVVADRLAPAELLAELPPHVEVIDAAKIPYG
                     RAMAQEAINGVLIERAKAGKFVVRLKGGDPFVFARGYEEVLACIGAGVPVTVVPGVTS
                     AIAVPALAGVPVTHRSITHEFVVVSGHVAPGDPESLVNWNALAAMSGTIVLMMAVERI
                     ELFADALLKGGRPAETPVLVVQQGTTAAERTVRTTLADAPKKIREEGVRPPAIIVIGA
                     VAGFAELKER"
     gene            complement(11422..>12050)
                     /locus_tag="VF874_18380"
     CDS             complement(11422..>12050)
                     /locus_tag="VF874_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX9177951.1"
                     /translation="SATAPVTVAIAGGKAFTFGYAEHVELLQAAGADVVAFDPLTDPL
                     PAGADALVLPGGFPEQFAAELAANDVARQQIRDLAASGAPVHAECAGLTYLVDELDGH
                     PMCGVLSGSARFTDHLTLGYRDAVAVTDSSIHAAGERTTGHEFHRTTVEFTDSYPPAW
                     VYKGHEIAVRDGAVHNGVHGSYLHTHPAASPEAVARFVAHAAASRLAG"
BASE COUNT         2082 a         4217 c         3917 g         1834 t
ORIGIN      
        1 cgatctaccg gctgcaccgt cgagaacggg ttgttgccga cgcggggggt gcgcggcgca
       61 ggcctcggac cgggggcggg accgggacgc gggccgccgg gagtgggagc tgggcgcgcc
      121 gggctgggcg cgggcgcgct cgccgccggt gtggacggct gcgccgttgc cggggcgggg
      181 gccgccggcg gagccgcggc ggctggcggt gccggggctg cagcagcagc cgccttcgcc
      241 ggaacggcct ccgccggggc ggtggcgacg ctggacgact tggccgctgc gccgttgccg
      301 tcctgcgcgg cagcctgagg ggctttgccg ccgccaaagg actcacgcag ccgccgggcg
      361 accggcgctt ccaccgttga ggaggctgac ttgacgaatt cgccttgttc gctcagccta
      421 gcgagaactt ctttactggt gacaccgagt tccttcgcca actcgtgcac acgggccttg
      481 cctgccacta cctctccatc tctcgaggcg acagctgcgg taggcgcgcc tcgggtttag
      541 ctacgacaca tggtcatcgg gacttcacgg tgtgctcatg ttcttcgcta cctgttctgt
      601 tgcccggggc ctcgagcgcg gtgatgtgct cgagggcctc ggatgtgtcc ggtggaccgg
      661 tgatgcgcaa cgctcggccg aaagctcgcc gccggatcgc tgcgcccaga cactgctggt
      721 cgggatgcag ccaggcaccc cgccccggca gattacctgc ggtgtcaacg gtcacggcgt
      781 actcgccatt cccggtcggc accgcaacca cgcgaagcag ttcgacggcc aactctcgtc
      841 tccggcatcc gatgcaggtg cgcaccggcc ccaggtcgga ggccgaggtc gcggccgatg
      901 ccgctccgga agccgaagtc tcgcgctgga tcacggctca gtctacgtca cacgattgcc
      961 tcgtccgaac caccgttggc gcattcgttc gggcgcaggc ccctcgcccg cggcgaatct
     1021 cactcaggct cagaaagaac ttcaattcgg caagtaggcc cgtgcgcgtg agcgaggcgg
     1081 gcatcgagcg tcaagagcgt cgcatcgaat gcctcagcta gcgccacgta caacgcatcc
     1141 cagcctctaa ccgatgcccg taactgccac gcgcggtcca acaaccaccg atgcccgaaa
     1201 cgctcgccag gccagtcacg aaggtcggct acagcttgcc ccgcggcggt accgtccatc
     1261 cgccctcgta ggtactgggc gcggatgacg ccgagcacct ccacatcgat gacatgcggg
     1321 gccgcatggt cgacatccgc agcgagccgg gctgcgattt caccggagcg gggtgtgtcg
     1381 gccaccacct cgaacaggca cgacgcatca acgatcagca tccggccact cgccgcgcca
     1441 ctcatccaaa gtcgtcaaca cctcagccgt ggacacggag gacggccgtc gacctgttcg
     1501 cgcaagccac tgcacaactg acggccgggc agccagccgg gccgcctcgc ggcgcagaag
     1561 ctccggcacc gtgatgcctt cctcgccggc gcggcgcacc aacgcggcat acacctggtc
     1621 gtcgatgtca cgaatttgca ccgtcttagg catatctgca tcatatcatg cgtttacgca
     1681 tgaacggtaa acatgtcagg ccgtcggctg gccttgggtg gcctcgttgt cgctgcggat
     1741 gtcgatccgc catccggtca gccgggccgc caacctagcg ttttgcccct ccttgccgat
     1801 cgccagcgac agctggaaat cggggacgac gacgcgtgcg gcgcgggcat tggggtcgat
     1861 gacgctgacc gacaccacct tggcggggct cagcgcgttg gctacgaagc gcgccgggtc
     1921 ctcgtcgtag tcgatgatgt caatcttctc gccggacagt tcgctcatca cgttgcgcac
     1981 ccgttggccc atcgggccga tgcaggcgcc cttcgcgttg agccccggca ctcgagagcg
     2041 caccgcgatc ttggagcggt ggccggcctc gcgggcgacc gcggcgatct cgaccgagcc
     2101 gtcggcgatt tcgggcacct ccaacgagaa gagcttgcgg accaggttcg gatgggtccg
     2161 cgacagcgtg atcaagggtt cgcgcgcccc gcgacttacg ccgaccacat agcagcgaag
     2221 acgctcgccg tgctcgtagc gttcgccggg cacctgctcg gcggccggga tcacgccctc
     2281 ggagcctttt gtctcgctgc ccatccgcac gacgacgagg ccgcgggcgt tggcgcgggc
     2341 gtcgcgctgg atgaccccgg cgaccacgtc gccttcccgg gccgagaact cgccgtaggt
     2401 cttttcgttc tcggcgtcgc gaagacgctg cagcatgact tggcgcgcgg tggtggcggc
     2461 gacgcggccg aacccctcgg gggtgtcgtc ccactcgctg atgacgttgc cgtcaacgtc
     2521 ggtctcccgc gcgatgacag tcacctcgcc ggtcttgcgg tcgatttcga tccgcgcctc
     2581 gggctggtgc ccctgggtat gccggtaggc ggtcaacaat gccgacttga tgatctccag
     2641 cgattcgccg actgacatcc ctcggtcgac ctcgattgcg tgcagcgcgg ccatatcgat
     2701 gttcatgtga ccacccccgc cagttccagt tcgcgcggat ttggcggtga gaactccacc
     2761 tgcacaacgg ctttgacaat gtcgccgagc gggatgtcac gaagcgccca cccagactta
     2821 tcgcgcagca ccacgcgcag cacgccgtcg tcggtgtcgc cgagacgccc ggtgaccgac
     2881 gacccgtcgg aaagcgtgag ctcggctttg cggccacgag cgcgacggaa gtgcttctcg
     2941 gaggtgaggg ggcggtagac gcccggtgag ctgacctcga gcacgtaact agaagcggcg
     3001 gcgccatcca agtcgtccaa cagtgccgac gctcgccggg acagcgcggc gatggtgtcc
     3061 aggtcgagcg gatcatcacc gtcggcgatc accgtgatcc gaggcggacg ggccttggag
     3121 tcgacgatta cttcctcgat ctcgtagcca gcgcgcgcga actcgtcatc gagtagctcg
     3181 atcacctgtt cctgcgacgg cagccccgtg gccacggcga gctcctcatc ttgagttgtc
     3241 cggatcaagc tccgcaatgc ggagccagca tccaacgata cgccagaagt ggctctctac
     3301 gcggcgatcg aaagttcagc aatcgtgctt atcgtcgcct cggtcctcga tttctgcacc
     3361 acggctgtgg atcggctaga tcgacgacca tggtgcagaa atcggcgcat ggcactatgt
     3421 cgccatgccc ggcgccgccc cacagatcag ccgtcgccgc gtgcttgtcg gggcagccgc
     3481 gcttgccgcg ctgggtgcct ctgcgtgtgg cagctccccc gagccgccac agcccgaccc
     3541 tcttgaagcc caactcgact tagcgcagcg cgacagccag atggcgcacg ccgccgccac
     3601 tgcggccggc ccgttctacg caccgccgct caacgtcgtc gccgacgaac gcgccgccca
     3661 cgcgaaggcg ctctcgaagg aggtcgcccg cgcggccggc gaaaccgcca cgtcgtcgac
     3721 aacatcacct ccaaccacgg ccgcacccgc gttgccgcca ccgtcacgag agggcgtcat
     3781 cgcggcgctg cgtgagtcag ccgacagcgc agccaaattg gcggtgaatc tcgatggtta
     3841 ccgcgctggg ctgctcggct ccatcgccgc ggcgtgcact gcatcggtga cggtcccgct
     3901 ggccatgaag gagccggcac aatgacgtcc ccgcagccaa gccccagcaa ggcgcccgag
     3961 tcggccgggg acaaagcgct ggccgaggcg attgcagccg aacacgcgac gatctatggc
     4021 tacggcttcg tctccgccca ctcccttcct gacctcaacg agatgatctc ggattccctg
     4081 gctgaacacc gtgcccgacg ggaggccgcg atcgcgatgc ttgcctcgcg tgaggtggcc
     4141 gcaccgatcg ccgcagtggg ctaccaactg ccgatccccg tcaacaactg gagagacgcg
     4201 gccaccctgg cggtcaggat ggaaaccgat gacgaggccg cctggcgtgc cgtgttggag
     4261 caggcggaat cgtcacagga ccgggccttc gcggtgacgg cgctcacgca ggccgcagtg
     4321 cgcgccgcgc gctggcgaca ggtgctgggg acgtggccgt tgacgtcgcc gttccccggg
     4381 ggcggcgaat aaccctcacc cagaaagggt gtcggcgatc tgcgtcgccg cggaatcgac
     4441 gggcacttcg cgggtttcgc caccgaaccg gttgcgcagc tccacaattc cgttggccca
     4501 gccgcggccg accaccacga tccacggcac acccaacagc tcggcgtcct tgaacttcac
     4561 cccgggcgag gcctgccggt cgtcgagcag cacgtcgaca cctagccggt ccaggtcgcg
     4621 cgccaactgt gccgcgccgt cacgcgcggc cgcgtccttg ttcgcgatca ccagatgcac
     4681 gtcgaacggc gacaccgacg ccggccaccg cagcccgagt tcgtcgtgat gctgctcggc
     4741 gatcaccgcg accagccgcg acaccccgac accgtaggag cccatcgtga gccgcactgg
     4801 cttgccgtcc tcgccgagaa cgtcgaccgt gaacgcgtcg gtgtacttgc ggccaagttg
     4861 gaagatatga ccgatctcga tgccgcgcgc cgataccagc ggcccggcgc cgtcgggtga
     4921 catgtcgcca tcgcgcacct cggcggcctc gatcgtgccg tcgggaagga agtcgcgacc
     4981 ggcgaccagc ccgacgacgt gcttgcccgg cgcgtcggcc ccggtgatcc agctggtgcc
     5041 gtccacgacg cgggggtcga cgaggaagcg aataccgttc ttcgccaatg cctttgggcc
     5101 gatgtagcct ttgaccagga aggggtactt ggcgaagtcg ccgtcgtcga gcatcgcgta
     5161 gtcggccggg tcgagcgcgg cgcccagccg cttgtcgtcg atctcccgat cgccgggaat
     5221 gcccaccgcg agaagctccc aatcgccgcc cggcggacgg actttcatca gcacgttctt
     5281 caacgtgtcg gcggcggtga cggtgcggtc cagagccgaa ttcgcccagt cgaccagcgt
     5341 gctgatggtc ggtgtatcgg gtgtgtcgtg caccaccggc tccggaaggt cgtcgaacgg
     5401 caacggattc ggccgggcgg tgaacacggc ctccacgttg gccgcgtagc cggattccgc
     5461 gcagcgaacg aaagtgtctt cgccgatggg gctttcggcg aggaactcct ccgatgcgct
     5521 accgcccatc gccccggtta ccgccgagac gatcacgtac cgcacgcgca tccggtcgaa
     5581 catccgttga taggcgtcgc ggtgcgcgtg gtactgggtt ttcagcccgg cgtcgtcgac
     5641 gtcgaacgag tacgagtcct tcatcacgaa ctcgcggccg cgcaggatgc cggcgcgggg
     5701 cctcgcctcg tcgcggtact tcgtctggat ttggtacagc agaagcggaa agtccttgta
     5761 ggagctgtac tcccccttca ccgtgagtgt gaacagctcc tcgtgggtgg gaccgagcat
     5821 gtagtcgttg ccgcgccggt ccttcaggcg aaacaccgct tcgccgtact cggtccagcg
     5881 gttcgtcgcc tcgtacggcg cccggggaag cagcgcggga aacagaatct cttgtccgcc
     5941 aatggcattc atttcctcgc ggacgatgct ctcgatcttg cgcagcacac gcagacccgg
     6001 cggcagccag gtgtacaggc ccggcccgat gggccggatg taccccccgc ggatcaacag
     6061 cttgtggctg ggcacctcgg cgtcggcggg gtcctcgcgc agcgtgcgca ggaacagctc
     6121 ggacatgcgg gtgatcacag ccgacaaccc tatgcgatgg ccggccgcca agcggacttc
     6181 aagaatccgt cgagaatccg aggaggcggg tgcccgaatc tcgtttcaac caaccacccc
     6241 gcctcggcgg gaaacgagaa aaggacacca ccaaatgagc accaccttca ccgcccgcat
     6301 caaggacgcg gcccgcgccg cgttagtcgc caccgcactc accggcgctg cgctcggctt
     6361 ggccggcacc gcccatgccg agctgtacgg caaccccgag gaagcggccg acttctggca
     6421 gcaccaagac ttcgacaact gcgtgctgat ggcgaccgcc gacatgatcg gcgaactgca
     6481 aggcgacatg atctccgaat tcgagatcac cgcgacggcc tcgatcaccc ccagcgtggt
     6541 acatcccggg acgatctaca tcccgcccat cggctccctc gacgacccca accaggggat
     6601 gggcaccgac cccatcgaca gcctggtgct gctggagaag tacggcttca gcggcaaggt
     6661 cggcggcgac ctcgccgaag aggtggacga ggaattcacc accgagaccg ggatggacgc
     6721 cctcgcccag tacctcgccg acgggcaaaa ggtcatggcg atcgtcaatg cccagttgat
     6781 ctggggcgac accgagggac cgttcgacga gcccaaccac gcgctcgtcg tcaccggcat
     6841 cgacaccgac gagggcgtcg tgcacctcaa cgacagcggc aatccggacg gccgggactt
     6901 ccagatcgac atcgagacgt tcgagcaggc ctgggctacc agcggtcaga gcatcgttgt
     6961 ctcggaccag acgtcctaac ccaacaccgc acaagggcgg ccggctgcat accagcagcc
     7021 ggccgcctct cgttttggca ctaaagctcg ccggcctcca cggcctcacg cgtgtactgc
     7081 gcggccgcga tctgccgggc ggagaagccg atccagaacg cgctgatgcc caccaggact
     7141 gcgacggccg ccacccacag cagcgagtag gtgtagccgt ggtccagcgc agtgagctgt
     7201 gcgggggtca tgtcccgcac cgatccggtg gtcccgccga gataaagagt gcgcgaggtc
     7261 tggacggcct ggatgacgac gagcaccagc ggcccgccga ggttctgcac catcagcgtg
     7321 atcgcggcga cgggaccgat ctcgcgcggg cccacgtcgg ccagcgcgca cagcggcaag
     7381 atcaccgaga tcacgccgat cccgaagccg ccgacgatga tcggcacgaa caggtccggg
     7441 aaatagggaa tgttgcggtt cagcgtcgac ccgtacagca tcgcgccgag cacgaacgag
     7501 ccaccgccaa tgatcagcca ccgcggagcg atcagcggcg ccacccgagc cgcagccacg
     7561 tttcccagcc cgagcgccac tgcgaacggg atgaacgaga tgccggcacg caacgcggag
     7621 tacccgagca cgacctgcac gtacagcccg atcatcacgg tcagcgtcaa cagcacgccc
     7681 ccggcgagaa acagcgagac gaacgtcatc acccggttgc ggtcggtgaa cagcgacagc
     7741 ggcaccagcg ggtgatctgc ggtgcgttcc accagcagaa accctccgaa cagcaggacg
     7801 gttgccgccg ccgcgccgaa gatccacgga ttgatccaac ccagtggcgg cccctgcgtg
     7861 aagatcagga ccgccgtcgt gcaggccagc gtcgccagca ccgcgccggt ggcgtcgagc
     7921 cgcagccgtt cgcggtgcgt ctcccgcagc cgggtcatcg cgatcgcgat gatcacgatg
     7981 ccgatcggca cgttgatcag aaacgccagc cgccacgaga tcaccgtgaa cgcgccgccg
     8041 agaaccaagc cgagcaccga gccgataccc atcattcccg ccgacaccgc catcgcctgg
     8101 ttgcgggcgt gcccgacggc gtacgtcgac gcgatcagcg ccagccccgt cggcgccgcg
     8161 accgcggcgc cgatgccctg cacggcccga gcggcgatca acatggccgg atcgaacgcc
     8221 acgccgcaga ccagcgacgc cgccgtgaac acgccgacgc cggcgatgaa cgcgcgcttg
     8281 tgccccactg cgtcaccgac gcgccccccg agcagcagca gcccgccgaa ggtcagcacg
     8341 tatgcggtga tcacccagct cttggccgcg tcggacatgc ccaagtcggc ctgcatgcgc
     8401 ggcagcgcga ccacgacgat cgtgccgtcg agcgtcgaca tcaactgcat gccggtgatc
     8461 gcgacgatcg cgatcccgac gacgctggaa gccaacggcc tcgacgaccg ttgggtgcgc
     8521 cgcgccgcga acatggctgc ctaccctacc gaggggtaac tgagccccgc tcgacgtcgg
     8581 gctagagctc tccggcgctg aatgtcgagc taaagctctc cggcgctgaa tgtcgagcta
     8641 aagctctccg gcgtccagcg cgtccttgac ttcttgcgcg tgtgcgacct gctgcgaggt
     8701 gtatccgatg aacagcgcag cggcgccgac cagcacggcc accgcggcca cccacagcag
     8761 tccgtaggtg tagccctggt cgagcgcatg cagctgggcg gcgttcatgt tcttgaccgg
     8821 gcccgtcgtg ccacctaggt acagcgtgcg cgacgtgatg acggcctgga ccacagccag
     8881 caccaccggg ccgccgaggt tctgtgccat cagcgcgatc gccgacaccg gtccgatccg
     8941 gtcgaacccg actcctgcga tggccgacac agtcagcggg accacgatca tcccgatgcc
     9001 gaacccgcct accgtgatcg gaagcaccag gttagggaag tacgggatac cgccgttgag
     9061 cgtcgagccg tagatcatcg cggccagcac cagcacgccg ccgcccatca ccagcatccg
     9121 cggcgggaac agcgacacca gatgcgacga cacgcccagc ccgatgccca gtgcgatcac
     9181 gaacggaatg aagccgacgc cggcgcgcag tgcgctgtag cccatgatgt cctgcacgta
     9241 caggccgatg agcacggtca gcgtgaacat cacgccgccg gcgaggaaga ccgccgcgaa
     9301 cgtcgcaacc cgattgcggt cgcggaacag gtctaacggc accagcgggt tctcggcggt
     9361 gcgctcggtg atcgcgaacg caccgaggaa caccactgcc gcgaggcccg aggcgagggt
     9421 caccggagac agccagccct gctcggggcc catcgagaag ccgaacacgg ccgcggtgca
     9481 gccaagcgtt gccaggatcg cgccggtggc gtcgagcttg atcggctcgc ggtgggtttc
     9541 cctcagcgtg gtgtaggcca gatagagcat caacagcccg atcgggacat tcaccaagaa
     9601 cgccagccgc caggacacct cggtcagcgc gccgccgacg atcaggccca tcaccgaccc
     9661 gataccggtc atcgcggcga agatcgccgt tgcggtgttg cgggccgggc ctttcgggaa
     9721 cgtggtcgcg atcaatgcga gcgcggtcgg cgatgcaatg gccgcgccga cgccctgcag
     9781 cagccgggcg atgaccaacg ttgcctcgtc ccaagcgagt ccgcacagca ccgaggcgat
     9841 cgtgaacagc gcgacgccgg cgatgaacgt gcgcttgcgg ccgatcgtgt cgccgagccg
     9901 gccgcctagc agcatcagcc cgccgaacgt cagcacatag gcggtgatca cccagctgcg
     9961 gccggcatcg gacaggttca actcgtcctg gatcctcggc agagcaacga tcgcgatcgt
    10021 gctgtccatc gtggcgagca gctgcatgcc gccgatggcg acgaccgcgg cgatgaaccg
    10081 ccgggacggc aaccacgccg gacgggacca gccggtacgt tgcgaaacct cgagggcggc
    10141 cttattggcg cgatccagag gggtcctccg ctccgcgtca tcgagagccg tcatggaggg
    10201 ttaccttaca gtactcttac cgttccttta attccgcgaa tcccgcgact gctccgatca
    10261 cgatgatcgc gggaggtcgg acgccctctt cccggatctt cttgggcgcg tcagcaagcg
    10321 tcgtgcgcac cgtgcgctcg gccgccgtgg tgccctgttg caccacgaga accggcgttt
    10381 ccgcaggtcg accgcctttg aggagcgcat cggcgaacag ttcgatccgt tcgacggcca
    10441 tcatcagcac gatggtgccc gacatcgcgg ccagcgcatt ccaattcact aacgattccg
    10501 ggtcgccagg ggcgacgtgc ccgctgacca caacaaactc atgcgtgatc gaccggtgtg
    10561 tgaccggaac gccggccaac gccggcaccg ctatggcgct cgtcacaccc ggcaccacag
    10621 tcacggggac gcccgcgccg atgcacgcca gcacctcttc gtagccgcgg gcgaacacga
    10681 acgggtcgcc gcccttgagg cggacgacga acttacccgc tttggcccgc tcgatcagca
    10741 cgccgttgat ggcctcctga gccatcgccc ggccgtacgg gattttcgcg gcgtcgatca
    10801 cctcgacgtg cggcggcagt tcggcgagca actcggccgg ggcgagccgg tccgccacga
    10861 ccacgtcagc gtgggccaac agccgtcggc cgcgcacggt gatcagttcg ggatcgccgg
    10921 gtccgccgcc gacgagggcg acaccgctgt gcgtcacctc cggcgtctcc gcagcgatga
    10981 tgccctgctg caatgcttcg tgaatcgccg acctgatggc ggcggagcgt cgatgctccc
    11041 cgcccgccag cacgccgacg gcgaggccct cgtattcgaa agacgccggg gtgacggcag
    11101 ttccttcccg ggcgatgtcg gcgcgcacgc agaagacgtg acgcgcttcg gcttcggcga
    11161 cgaccgcggc gttgatgtcc ggttcgtcgg tcgccgcgat cgcgtaccag gccccgtcga
    11221 ggtcgccgtc gcgatactct ctgagctcca acgagattcc gctcatcgct tcgacagccg
    11281 gggtcgcgga ccgggtgatg acgtgcacgt cggcgccgct ggcgatgagc agcggtaggc
    11341 ggcgctgcgc gacgctgccg ccaccgacca cgacgacctt cttgccgccc agccgcagcc
    11401 cgaccagata cgggttctcg gtcacccggc gagcctagag gccgcggcgt gggcgacgaa
    11461 gcgcgccacc gcctccggcg aggcagccgg atgagtgtgc agataggagc cgtgcacgcc
    11521 gttgtgcacg gcaccgtcgc ggactgcgat ctcgtggcct ttgtacaccc aggccggcgg
    11581 gtagctgtcg gtgaattcaa ctgtggtgcg gtggaattca tgcccagttg tgcgttcgcc
    11641 cgcggcatgg atggacgagt cggtgaccgc gacggcgtcg cggtagccga gcgtcaggtg
    11701 gtcggtgaac cgcgccgaac ctgacagcac gccgcacatc gggtgaccgt cgagttcgtc
    11761 gaccagatag gtcaggcccg cgcattccgc atgtaccggc gcgccgctcg cggcaaggtc
    11821 gcgaatctgt tggcgtgcaa cgtcgttggc agcgagctcg gcggcgaact gctcgggaaa
    11881 tcctcccggc aggacgagcg cgtcggcgcc tgccggcagc ggatcggtga gcgggtcgaa
    11941 cgccacgacg tccgcaccgg ccgcctgcag cagctcgacg tgttcggcat aaccgaatgt
    12001 gaacgccttg cctcccgcga tcgcgacagt caccggcgct gtcgcgctgt
//
LOCUS       DASWBF010000501         4704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_380197,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000501 DASWBF010000000
VERSION     DASWBF010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380197"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..572
                     /locus_tag="VF874_18385"
     CDS             15..572
                     /locus_tag="VF874_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014943072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177952.1"
                     /translation="MTGYGDGVLSDARIAAMSPAERRDLIQRLQRPLDEVFPPPIARP
                     MRRNRLTLMVGGAIGLIPWMVYLGFTLPANYVAHNWPATWIGFDCVLIAFMAVTAVLG
                     WLRRQLVLLPAFTTGVLLICDAWFDIMTAGPGQIWVSVLTAALGNLPLAMIMITGAVR
                     ILRLTATRLWFLDPGTPSWRLPLVP"
     gene            788..2002
                     /locus_tag="VF874_18390"
     CDS             788..2002
                     /locus_tag="VF874_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9177953.1"
                     /translation="MVEHDERVRDLAGLTVPLTGSLEDTGDAWLPYRLVDPVGEPVEA
                     VSAYFRDLQAIGRSTATIRSYGLDLLRWFRFLWAIGIAWNRATRVESRDFCRWMLVAG
                     KPSRPHWRRPREIPERAGVVAYAPSVRAHAETVLRSFYGFHLEAGTGPIVNPFPLARP
                     RAGRAHPHHNPMEPYRNERAGLYRPRVPSRIPRSVPDAEFNEIFARLPSHRDRALVAF
                     YVSTGARASELLSVTQGGVDPGRQLITVTRKGTRETQELPASSDAFVWLRLYQLEMDG
                     LIPRGKRQPLWWTSRLPLRLLTYHAAHRMFERVTERAGTAATLHSLRHTAAYRMAEDP
                     RLPLTDVQLVLGHAQLTTTQIYLTPRQEDVVRRVLAHHDEQTRQATARSRPAPAPGYR
                     PESLDVLFGGHG"
     gene            2002..4431
                     /locus_tag="VF874_18395"
     CDS             2002..4431
                     /locus_tag="VF874_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017682315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9177954.1"
                     /translation="MTIELVEPAVREPIPPAPVDDPGDTRFPARVPATAWPATTAGRD
                     EVTARLSRPPLVLGGAASQKHRSRGLGLLLDWLSDQPGDSWQQRWLASGVDAAAADWR
                     RVPADWLRRHDHVNPGRHAAMCAAVAVAIAADLVRPSPAWLVAAGTRSLVVVHDTMEA
                     RDPDGFARLRRLCDSDPLVSARGRDHTLHRAALIVAAKGGMLGEITIGDVLELLDTEA
                     RIHRQPRTHGADFYRMMRCIGVFGPSTPHRLGELRSIGQRTPEELIDRYQLACRPVRD
                     LLVEYLKERQPGMDYNSLRKLAACLGNMFWKNLERHHPGIDNLHLPAGVAKAWKQRLR
                     TMSKTVDTADEKHTVTVERVSYRQCLTPVRAFYLDLAQWALEDPARWARWVAPCPVND
                     EEINQRKLARHRKARMDTRTRERLPVLPELIRSVAEQRSATQVSLRAAQQTRPGQNFT
                     AAGQTLTRASVTRTTGERIWADDPATRRRRDLTTEEDLAFWAWAMVEVLRATGIRVEE
                     LLEISHHSMVQYRLPTTGELVPLLQIAPSKTDAERLLVISPELADVLSAIIGRIRGDA
                     AAIPLVPAYDRRECVWQAPTPWLFQRRFGGEQRRMSDGAVRNILATALAHTGLTDREG
                     GPLRFTPHDFRRIFITDTIMSGLPPHIAQIIAGHRDINVTLGYKAVYPDEAVQAHLAF
                     LARRRALRPSEEYRVPTDDEWAQFLGHFERRKVSIGTCGRASGTPCIHEHACVRCALL
                     WPDPSQRDRLHEIRDNLIARIADAEHEGWLGEVEGLRVSLAGAEDKLAQIDRRTATTT
                     ELGIPSLPTKP"
     gene            complement(4477..4704)
                     /locus_tag="VF874_18400"
                     /pseudo
     CDS             complement(4477..4704)
                     /locus_tag="VF874_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          740 a         1589 c         1584 g          791 t
ORIGIN      
        1 gatactcaag agtcatgact ggctacggcg atggtgtgct gtcggatgcc cgcatcgcgg
       61 cgatgtcgcc cgctgaacgc cgcgacttga ttcagcggct gcagcgtccg cttgacgaag
      121 tgtttccacc accgatcgcg agacccatgc ggcggaaccg gttgaccttg atggtgggcg
      181 gcgctatcgg gttgatcccg tggatggtct acctcggctt caccctgccc gcgaattatg
      241 tcgcccataa ctggccggcg acgtggatcg gcttcgactg tgtgctaatc gcgttcatgg
      301 ccgtcaccgc ggtgctgggc tggctgcgcc ggcagctggt gctgctcccc gcattcacga
      361 caggggttct gctgatctgt gatgcctggt tcgacatcat gaccgccggc ccgggccaaa
      421 tttgggtgtc ggtgttgacc gccgccctcg gcaacctgcc gctcgcgatg atcatgatca
      481 ccggcgcagt gcgaattttg cggctgacag cgacacggct ctggtttctc gaccccggga
      541 cgccgtcgtg gcgcctgccg ttggtgccgt aaccgggcag ggcctcggca aagtcgcatt
      601 tcagcagctc atcagtagtt tgatgggccg gtgatggtcg cgggcgtggt gtcgcagtgc
      661 ggcggcgatg ttgtcgtggc cggtgagtcg gagcaggccg atcacggtgt tacttgttga
      721 ctgaacttcg acacgcgttt gcccagcttg acgggcgatt gtcggtgtgt tggttatcgt
      781 ccgcctcatg gttgaacatg acgagcgcgt ccgggatctg gccgggttga cggtgccgtt
      841 gactggctcg ctggaggaca cgggcgatgc gtggctgccg tatcgtctgg tcgacccggt
      901 cggtgagccg gtggaggcgg tgtcggcgta cttccgtgac ctgcaggcga tcggccggtc
      961 gacggcgacg atccgctcct acggtttgga cctactgcgc tggtttcggt tcctgtgggc
     1021 gatcgggatc gcgtggaacc gggccacccg ggtagaatca cgcgacttct gccgctggat
     1081 gctggtggcg ggcaagcctt ctcgcccgca ctggcggcga ccgcgggaga tacctgagcg
     1141 ggctggggtg gtggcgtacg cgccgtcggt gcgtgcgcac gccgaaacgg tgctgcgcag
     1201 cttctacggc tttcacctgg aggcgggcac cgggccgatc gtcaatccgt tcccgctggc
     1261 ccgcccgcgc gccggccggg cccacccgca ccacaatccg atggagccct accgcaacga
     1321 gcgggccggg ttgtatcggc cgcgggtgcc gtcgcggatc ccgcgcagcg tgccggatgc
     1381 tgagttcaac gagattttcg cccggctgcc ctcgcaccgg gatcgggcat tggtcgcgtt
     1441 ctacgtgtcc accggtgctc gggcctcgga gttattatcg gtcacccaag gtggcgtcga
     1501 tccggggcgg cagctgatca cggtgacccg caagggaact cgcgagactc aggagctacc
     1561 ggcgtcgtcg gacgcgttcg tgtggctgcg gctctaccag ctggagatgg acgggctgat
     1621 cccgaggggg aagcggcaac cgttgtggtg gacttcgcga ctgccgttgc ggctgttgac
     1681 ctatcacgct gcgcatcgca tgttcgagcg ggttaccgag cgggccggca cggcggcgac
     1741 gctgcactcg ctgcggcata ccgcggccta ccggatggcc gaggatccgc ggctcccgtt
     1801 gaccgatgtg cagctggtgc tcggccacgc ccagttgacc acgacgcaga tctatctgac
     1861 gcctcgccag gaggatgtgg tgcgtcgggt gctcgcccat cacgacgaac agacccggca
     1921 ggccaccgca cgcagcagac ccgccccggc gccgggatac cggcccgagt cactggacgt
     1981 gctgttcgga ggccatggct gatgacgatc gagctcgtcg agcccgccgt gcgtgagccg
     2041 atcccgccgg cgccggtgga cgatccgggc gatacccggt tccccgcgcg tgtgccggcc
     2101 acggcgtggc cggcgaccac cgccggccga gacgaggtca cggcccggct gagccgcccg
     2161 ccgttggtgc tcggcggcgc cgccagccag aagcatcggt cgcgagggct ggggttgctg
     2221 ctggactggt tgagcgacca gccgggcgac agctggcagc agcggtggtt ggccagcggt
     2281 gtcgacgccg ccgcggcgga ctggcgacgg gttcccgccg actggttgcg ccgccatgac
     2341 cacgtcaacc cgggccgcca tgccgcgatg tgcgcggcgg tcgcggtggc gatcgccgca
     2401 gacctcgtcc gcccgtcgcc ggcctggctc gtcgcggccg ggacacgttc gctggtcgtc
     2461 gtgcacgaca caatggaggc ccgcgacccg gacgggttcg cccgcctgcg tcgactctgc
     2521 gacagcgatc ccctggtgtc tgcgcgggga cgtgatcaca ccctgcatcg cgctgctctg
     2581 atcgtcgccg ccaagggcgg catgctgggc gagatcacca tcggcgacgt gctggaactg
     2641 ctggacaccg aggcccgtat ccaccggcag ccgaggaccc atggcgccga cttctaccgg
     2701 atgatgcgct gcatcggggt attcggtccc tcaaccccgc accgactggg tgagctgcgc
     2761 tccatcggtc agcgcacgcc ggaggaactg atcgaccgct accagcttgc ctgccgaccg
     2821 gttcgcgacc tgctggtcga gtacctgaag gagcggcagc cgggcatgga ctacaacagc
     2881 ctgcgcaagc tggccgcctg tctgggcaac atgttctgga agaacctcga gcgccaccac
     2941 cccggcatcg acaacctgca cctgcccgcc ggcgtcgcca aggcgtggaa gcagcgcctg
     3001 cgcaccatgt cgaagaccgt cgacaccgcc gacgagaagc acaccgtcac cgtcgaacgg
     3061 gtcagctacc ggcagtgttt aaccccggtg cgggccttct acctggacct ggcccagtgg
     3121 gcactcgagg accccgcacg ctgggcccga tgggtcgcgc cctgcccggt caacgatgag
     3181 gagatcaatc aacgcaaact ggcgcggcac cgtaaggcac gcatggatac ccgcacccgt
     3241 gaacggctgc cggtgcttcc cgagctaatc cgcagcgtcg ccgaacagcg cagcgcaacg
     3301 caggtctcgc tgcgcgccgc gcaacagact cgacccgggc agaacttcac cgccgccggg
     3361 cagaccctga cccgagcgag cgtgacacgc acgaccggcg agcggatctg ggccgacgac
     3421 cccgccacca gaagacgacg agacctgacc accgaagaag acctcgcgtt ctgggcctgg
     3481 gccatggtgg aagtgttgcg cgccaccgga atacgcgtcg aggaactcct ggagatcagc
     3541 caccacagta tggtgcagta ccggctgccc accaccggcg aactggtgcc actcctgcag
     3601 atcgccccgt ccaagaccga cgccgaacgg ctcctcgtga tcagccccga actcgccgat
     3661 gtgctgtcgg ccatcatcgg ccgtatccgc ggcgacgccg ccgccatccc gcttgtgccc
     3721 gcctacgacc gacgagaatg cgtgtggcag gcgcccacgc cgtggctgtt ccagcgccgg
     3781 ttcggcggcg aacagcgcag aatgtccgac ggtgcggtcc gcaacatcct cgctaccgct
     3841 ctcgcccaca ccggcctcac cgaccgtgag ggcggcccgc tgcgcttcac accgcacgac
     3901 ttccgccgga tcttcatcac cgacaccatc atgagcggtc tgccgccgca catcgcgcag
     3961 atcatcgccg ggcatcgcga catcaacgtc accctcggct acaaggccgt ctaccccgac
     4021 gaagccgtcc aggcccacct tgcgttcctc gcccgccgtc gcgcactgcg tccctccgag
     4081 gagtaccgcg tccccaccga cgatgaatgg gcccagttcc tcggtcactt cgaacgacgc
     4141 aaggtctcca tcggcacctg cggccgcgcg tccggaacgc cctgcattca tgaacatgct
     4201 tgtgtccgtt gcgcattgtt atggccggac ccctcgcaac gggaccgatt acatgagatc
     4261 cgcgacaact tgatcgctcg tatcgccgac gccgagcacg aaggctggct cggcgaggtc
     4321 gaaggtctgc gggtcagcct cgccggtgcg gaggacaagc tcgcccagat cgaccggcgg
     4381 accgcgacga ccaccgaact cggcataccc tcgctaccaa caaagccgtg aatgcaacga
     4441 acggatcatc aagcggcacc atcgagttca gagaatgcgc agcgtggcca tgacctgggg
     4501 gccggcgccg gttctgatct gcgaatgatc ctctgcgtag gtgacatcgc ggacccagtg
     4561 cagccgattc tcgatgtgcc agtggccgcg cagccagtcg gcgatgtggc cgggctgggc
     4621 gtgggtggcc gagagcgagg cgtagacgac ttcggtgtgg cgtgtgcggt tgttggcgtt
     4681 ggtgacggtg cgggtcagtt gcag
//
LOCUS       DASWBF010000502        13180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1035596,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000502 DASWBF010000000
VERSION     DASWBF010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13180
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035596"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VF874_18405"
     CDS             complement(<1..525)
                     /locus_tag="VF874_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX9177955.1"
                     /translation="MDVEVVGKFLSTLREDDDHPYRTGPWRPQTTEWRADDLPTVEGE
                     VPSDLDGVYLRNTENPLYSALKFYHPFDGDGMIHVVGFRDGKAFYRNRFIRTEGFLAE
                     NEAGRPLWAGLAEPPKLAERDGWGARGRMKDASSTDVIVHRGTALTSFYQCGDLYRVD
                     PFSAETLGKETWDGR"
     gene            complement(515..1732)
                     /locus_tag="VF874_18410"
     CDS             complement(515..1732)
                     /locus_tag="VF874_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX9177956.1"
                     /translation="MTTSAVWLLGGYQTDFARNFSREGLDFADLTGEVVRHTLESARV
                     GAEDIGVVHVGNAFGELFTGQGHLGAMPATVCADLWGVPAIRHEAACASGSAAALAAI
                     ADLRSGAYASALVVGVELEKTVPGDTATRYLGAAAWIGHEGDGAKFMWPFMFSDIADE
                     YDRRYGLDDAYLRAIAELNFANARKNPNAQTRDWQVPDLAVAEANPVIEGRLHRFDCS
                     QVTDGGAGVVLVSDEYLRAHPDVRPVARILGWGHRTVGLGLRQKLDRSAESPYVLPHL
                     RTTVQDAFDRAGVTLDDVDGFEVHDCFTPSEYLAIDHIGLTPPGQSWQAVESGDITIG
                     GRLPINPSGGLIGGGHPVGASGVRMLVDAARQVSDSAGDYQIDGARTFGTLNIGGSTA
                     TAVSFVVGAGDGR"
     gene            1764..2945
                     /locus_tag="VF874_18415"
     CDS             1764..2945
                     /locus_tag="VF874_18415"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9177957.1"
                     /translation="MARLLPLLCVLLVGCGAPLTAPPTASPDKAKAEAVLKIVRDTMA
                     ERHLKAVIVRVTVGGKEVLTQAVGDSMTGVPATPQMHFRNGAVAISYVSTLLLKLVDE
                     KKVRLDDKLSRWLPDIPNADRVTLGQLAQMTSGYPDYVLGNEAFSAALYADPFKQWTT
                     DQLLAQVLSRPLLYPPGTNWNYAHTNYLLLGLALEKLTGEDMPKLLSDKVLKPLGLSQ
                     TANSDTPEIPSPVLHAFSAERGKYEETTFWNPSWTITHGAIQTTNIYDLEATARGIGS
                     GRLLSPESYKQMVSTDLRGKTRAQPGCPTCAEQNDIYTYGLGVVISGNWLLQNPMFAG
                     YAGVEAYLPSQKMALAVVTTFTEQAFDAQGNYDNEADTLFRRIGAELAPGDAPPHPPA
                     K"
     gene            2952..3659
                     /locus_tag="VF874_18420"
     CDS             2952..3659
                     /locus_tag="VF874_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX9177958.1"
                     /translation="MTRKVLFIVNDPVATEALLGEAFADNGFDADTSGVVPWTRVDDP
                     AVEVTFPDPAGYDVIVPLGARWPVYDDALRRTWVGAEMRLLCDAHDAGVAVLGVCFGG
                     QLLATAHGGSVTRSGAPELGWFELDTDDPALVPPGPWFQWHFDSWTLPTGATEISRNA
                     QGSQAFLLGRSLALQFHPELDGALLSRWIADDRDRDIETLGIDENELIARTAELEDDA
                     RRRVHQLVRGFIARVGG"
     gene            complement(3637..4919)
                     /locus_tag="VF874_18425"
                     /pseudo
     CDS             complement(3637..4919)
                     /locus_tag="VF874_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
     gene            4971..5618
                     /locus_tag="VF874_18430"
     CDS             4971..5618
                     /locus_tag="VF874_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX9177959.1"
                     /translation="MRGGTFADRREAGRVLADALAAYRGAPGLLVLGLPRGGVPVAWE
                     VAAALRAPLDVFVVRKLGAPRWEELAVGALASGGLVVLNDDVVRSLRISDEQLQRVID
                     KEAAELSRREDAYRGGRPPIEPAGKTVILVDDGIATGASMLAATRAIRAAGPAKIVVA
                     VPVAPPSACRQLADAADNVVCVQVPPSFLAVGEYYRDFRQTSDDEVRELLATPTR"
     gene            complement(5621..5938)
                     /locus_tag="VF874_18435"
     CDS             complement(5621..5938)
                     /locus_tag="VF874_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX9177960.1"
                     /translation="MLIVALVMAVIGLAALVTAVVTSNEIIAWVCIGASVFGVILLIV
                     DALRERQKRDAAPAAESPEDDRDDEEFDAEYPDEEQPSLSEQADQTESADPESTEENP
                     AER"
     gene            complement(5951..6772)
                     /locus_tag="VF874_18440"
     CDS             complement(5951..6772)
                     /locus_tag="VF874_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX9177961.1"
                     /translation="MPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVEAELTRLIE
                     ENTDLRQRVAELDQELAGARSGSGVATSQAMPMYEQPPEPPQQPVYEAPPPAPPQPEP
                     AGPIDEESPLKAARVLALAQDTADRLTGTAKAEADKLLADARANADSILSEARHTADT
                     TVAEARQRADAMLGDAQMRSETQLRQAQEKADALQADAERKHTEIMGTINQQRTVLEG
                     RLEQLRTFEREYRTRLKTYLESQLEELGQRGSAAPVDSSANAEGAAFNQFSRGNN"
     gene            complement(6935..7225)
                     /locus_tag="VF874_18445"
     CDS             complement(6935..7225)
                     /locus_tag="VF874_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX9177962.1"
                     /translation="MRLLFEILGLVLFVFWLLLIARVVIEFIRSFSRDWRPKGATVVI
                     LEIIMTLTDPPVKLLRRLIPQLTVGSIRFDLSIMVLLLVVFIGWTSLLNAGA"
     gene            complement(7291..7926)
                     /locus_tag="VF874_18450"
     CDS             complement(7291..7926)
                     /locus_tag="VF874_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX9177963.1"
                     /translation="MSTLHKVKAYFGMAPMEDYDDEYYDDDERGRGYSRRSQGDRFAD
                     DGYGRRYEGRDREYDDRDPDDYPPGYRGGYDEPRFRPRDYERPPSSRFGSLRGATRGA
                     LAMDPRRMAMLFEEGSPLSKITTLRPKDYSEARTIGERFRDGTPVIIDLVTMDNADAK
                     RLVDFAAGLAFALRGSFDKVATKVFLLSPADIDVTAEERRRIAEAGFYAYQ"
     gene            complement(7992..8744)
                     /locus_tag="VF874_18455"
     CDS             complement(7992..8744)
                     /locus_tag="VF874_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX9177964.1"
                     /translation="MREEHRLKRREKQLAASLAAVQRRIADAAAAAGRNAAEIELLPV
                     TKFFPASDVAILSKLGCRSVGESREQEAAAKSAEVATLVGESLRWHMVGHIQRNKARS
                     IAKWAYAAHSVDSTRLVAALGRAVAAALGTGQRRDPLEVFVQVSIDGDPTRGGVDARR
                     PELVDEVCDAVASANSLSLVGLMAIPPLDSNPDEAFAKLENEHLRVLSRHRDATRLSA
                     GMSADLEAAVKHGSTCVRVGTALLGPRPLTSP"
     gene            complement(8749..9462)
                     /gene="pgeF"
                     /locus_tag="VF874_18460"
     CDS             complement(8749..9462)
                     /gene="pgeF"
                     /locus_tag="VF874_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX9177965.1"
                     /translation="MGHRIRRVTTTRAGGVSKPPFDTFNLGDHVGDEPEAVAANRKRL
                     AAATGLDEDHVIWMNQVHGDRVERVDGPRTTPVEGTDALVTTTPRLALAVITADCVPV
                     LMADARAGVVAAVHAGRVGAQRGVVARTLEAMQAAGAHPDDVSVLLGPAVSGPNYEVP
                     EEMAAEVETALPGSRTTSCQGTAALDLRAGIARQLTDLGVTAVDADPRCTVEDPSLFS
                     HRRDAPTGRLASLIWMELR"
     gene            complement(9505..10638)
                     /gene="ftsZ"
                     /locus_tag="VF874_18465"
     CDS             complement(9505..10638)
                     /gene="ftsZ"
                     /locus_tag="VF874_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9177966.1"
                     /translation="MTPPHNYLAVIKVIGIGGGGVNAVNRMIEQGLKGVEFIAINTDA
                     QALLMSDADVKLDVGRDSTRGLGAGADPEVGRKAAEDAKDDIEELLRGADMVFVTAGE
                     GGGTGTGGAPVVATIARKLGALTIGVVTRPFSFEGKRRSDQAEAGINALRESCDTLIV
                     IPNDRLLQMGDAAVSLMDAFRSADEVLLNGVQGITDLITTPGLINVDFADVKSIMSGA
                     GTALMGIGSARGDGRSLKAAEIAINSPLLEASMEGAQGVLMSIAGGSDLGLFEINEAA
                     SLVQEAAHPDANIIFGTVIDDSLGDEVRVTVIAAGFDATSARSKRVVGVGAKVAAGSA
                     GTVTSTLFEPADAASVPVHTNGSSVRIGGDDDDDVDVPPFMRR"
     gene            complement(10774..11640)
                     /locus_tag="VF874_18470"
     CDS             complement(10774..11640)
                     /locus_tag="VF874_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX9177967.1"
                     /translation="MTEPLPGEVSTPDEESEELEGPRRRARREREQRREAAARARAIE
                     EARREAKRKATASATAPKPVGRGVVRGLKVLTWAVILTVVSVGLGLVLYFTPVMSVRN
                     LEIEGLEAIPRDEVVAAVAVRTGTPLLQVDTDKVANRVATISRVASVRVQREYPSSLR
                     VTVAERIPVAVKDMPDGTHLFDRDGVDFATAPPPPGVPYLDVDNPGPTDPPTKAALQV
                     LTALRPEVAGQIGRIAAPSVASITLTLVDGRVVIWGTTDRTDEKAEKLAALLTRPGHT
                     YDVSSPDLPTVK"
     gene            complement(11637..13139)
                     /gene="murC"
                     /locus_tag="VF874_18475"
     CDS             complement(11637..13139)
                     /gene="murC"
                     /locus_tag="VF874_18475"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX9177968.1"
                     /translation="MTPQPLPPELARVHMVGIGGAGMSGIARILLDRGAQVSGSDAKE
                     SRGVLALRARGAVVRIGHDPSSLDLLPGGPTVVITTRAAIPKNNPELVEARRRGVPVV
                     LRPRVLAMLMTGYRTLTVTGTHGKTSTTSMLIVALQHCGLDPSFAVGGDLGEAGTNAH
                     HGSGDYFVAEADESDGSLLEYAPDVAVVTNIDVDHLDFFGSAQAYTAVFDDFADRVAP
                     GGALVVCTDDPGAAALADRAAARGTRVLRYGSDEPLGRRADSPAERAEMAGRLLSWEP
                     QGTGAVAHVQLAGEALPRVMRLSVPGRHMALNALGSLVAAVEAGAEPECVLDGLAGFE
                     GVRRRFDLVGVANSVRVYDDYAHHPTEIRATLTAVRTLIEQGPDRSGRAIAVFQPHLY
                     SRTELFARDFGTALNSADLVFVLDVYGAREQPMAGVSGALVAEHVSVPVSYVPDFSAV
                     ADRVAEVTAPGDVVVTMGAGDITMLGPEILTSVQVKANRSAPGRSGVMRG"
BASE COUNT         2031 a         4686 c         4365 g         2098 t
ORIGIN      
        1 ccgcccgtcc cacgtctcct tgcccagcgt ctcggccgag aacggatcga cccggtacag
       61 gtcgccgcac tggtagaagc tcgtcaacgc ggtgccgcgg tgcacgatca cgtcggtgct
      121 cgacgcgtcc ttcatccgcc cgcgcgcgcc ccacccgtcg cgttcggcca gcttcggcgg
      181 ctccgccagc cccgcccaca ggggccggcc cgcttcattc tcggcgagaa agccctcggt
      241 gcggataaag cggttgcggt agaacgcttt tccatcccgg aagccgacga cgtgaatcat
      301 cccatcgcca tcgaacgggt gatagaactt cagcgccgaa tacagcggat tctcggtgtt
      361 gcgcaggtac accccgtcga ggtcgctcgg gacttcgccc tcgacggtcg gcagatcgtc
      421 ggcccgccac tccgtcgtct gcggccgcca cggcccagtc cggtacgggt ggtcgtcgtc
      481 ctcccgcagc gtcgacaaga acttgccaac aacctcaacg tccatcaccg gcccccacca
      541 cgaaactgac tgccgttgcc gtgcttccgc cgatgttgag tgtgccgaac gtgcgcgcgc
      601 cgtcgatctg gtagtcaccc gcggagtcgc tgacctgtcg ggccgcgtcg acgagcatcc
      661 gcacgcccga cgccccgacg ggatgaccgc cgccgatcag cccgccactc gggttgatcg
      721 gcaaccgccc gccgatagtg atgtcgccgc tctcgaccgc ctgccacgac tggcccggcg
      781 gtgtcaaccc gatgtggtcg atcgccagat actcgctggg cgtgaagcag tcgtgcacct
      841 cgaacccgtc cacgtcgtcc agcgtcaccc cggcccggtc gaacgcatcc tgcacggtcg
      901 tccgcaaatg gggcagcacg tacgggcttt cggcggaccg gtccagcttc tgccgcaaac
      961 caagtcccac agtcctgtgc ccccacccca gaatccgcgc caccggccga acgtccggat
     1021 gcgcccgcag atactcgtcg gataccagca ccacgcccgc gccgccgtcg gtgacctggc
     1081 tgcagtcgaa ccggtgcaac cggccttcga taaccgggtt cgcctcggcc acagcgagat
     1141 ccggcacctg ccagtcgcgg gtttgcgcgt tggggttctt gcgcgcgttc gcgaagttca
     1201 gctccgcgat cgcccgcagg tacgcgtcgt ccagaccgta ccggcggtcg tattcgtcgg
     1261 cgatgtcgct gaacatgaac ggccacatga actttgcgcc gtcgccctca tgcccgatcc
     1321 acgccgcggc accgagataa cgcgtcgcgg tgtcccccgg caccgtcttt tccagctcga
     1381 caccgacgac cagcgcgctc gcgtaggccc ccgagcgcag gtctgcgatc gcggcgagcg
     1441 ccgccgcgct gcccgacgcg cacgccgcct cgtgtcggat cgcgggaacg ccccacagat
     1501 cggcacagac ggtcgcgggc atcgcgccca ggtgcccctg acccgtgaat agctcaccga
     1561 acgcgttgcc gacgtgcacc accccgatgt cttcggcgcc gacgcgagcc gactcgagcg
     1621 tgtgccgcac cacctcgcca gtcagatcag caaagtccag gccttcccgg ctgaaattgc
     1681 gcgcgaagtc cgtctggtag ccgccgagca gccacaccgc tgacgtggtc atggccgtag
     1741 gctactccgc tgtggcactt gcgatggcac ggctcctccc cctgctctgc gtgctgctcg
     1801 tcggctgcgg cgcaccgctg accgcgccgc cgaccgcgtc ccccgacaag gccaaggccg
     1861 aggccgtgct gaagatcgtg cgcgacacga tggctgagcg tcacctcaag gcggtcatcg
     1921 tccgcgtcac cgtcggcggc aaggaagtgc tgacccaagc tgtcggcgat tccatgacgg
     1981 gcgtgccggc gactccgcag atgcacttcc gcaacggcgc ggtcgcgatc tcgtacgtgt
     2041 ccacgctgct gctgaaactc gtcgacgaga agaaggtccg cctcgacgac aagctgtcga
     2101 ggtggctgcc cgacatcccc aatgccgacc gggtcacgct gggtcagctg gcgcagatga
     2161 cgtccggata tccggactac gtgctcggca acgaggcgtt cagtgcggcg ctgtacgccg
     2221 acccgttcaa gcagtggacc accgaccagc ttctggcgca ggtgctttcg cggccactgc
     2281 tctacccccc gggcaccaac tggaactacg cccacaccaa ctaccttctg ctggggctgg
     2341 cgctggagaa gctgactggc gaagacatgc caaagctgtt gagcgacaag gttctcaagc
     2401 cgctcggtct ctcccagacc gccaactccg acacgcccga gatcccgtcg ccggtgttgc
     2461 acgcgttctc ggccgagcgg ggaaagtacg aggagaccac gttctggaac ccgtcgtgga
     2521 cgatcaccca cggcgcgatc cagaccacca acatctacga cctcgaggcc accgcgcgcg
     2581 gcatcgggtc cgggcggctg ctgtcgcccg aatcgtacaa gcagatggtg tcgacggacc
     2641 tgcgcggcaa gacccgggcc cagccaggct gcccgacatg cgctgagcag aacgacatct
     2701 acacctacgg gctgggcgtc gtgatctccg ggaactggct gctgcagaac ccgatgttcg
     2761 ccgggtacgc cggcgtggag gcgtacctgc cgtcgcagaa gatggcgctc gccgtcgtca
     2821 ccaccttcac cgaacaggcc ttcgacgcgc aagggaacta cgacaacgag gccgacacgt
     2881 tgttccgcag gatcggtgcc gagctcgcgc ccggcgacgc gccaccgcac ccgcccgcga
     2941 aataggtcgc cgtgacgcgc aaggtcttgt tcatcgtcaa cgatccggtg gccaccgagg
     3001 cattgctcgg agaagcgttc gccgacaacg gcttcgacgc cgacacgtcc ggggtggtgc
     3061 cctggacgcg ggtcgacgat cccgcggtgg aggtaacctt ccccgacccc gcgggctacg
     3121 acgtcatcgt gccgctgggt gcgcgctggc cggtctacga cgacgcgctg cgccgcacct
     3181 gggtgggcgc cgagatgcga ctgctgtgcg acgcgcacga cgccggggtg gcggtgctcg
     3241 gcgtgtgctt cggcggtcag ctgctcgcca ctgcgcacgg tggatcggta acccgttcgg
     3301 gtgcacccga actcggctgg ttcgagctcg acaccgacga ccccgcgctg gtgccgcccg
     3361 gcccgtggtt tcagtggcat ttcgatagct ggaccctgcc taccggcgca actgagatct
     3421 cgcgaaacgc tcaggggtcg caggcgttcc tgctcggccg cagtctggcg cttcagttcc
     3481 acccggagtt ggatggagcg ctgttgtcac gctggatcgc cgacgaccgc gaccgcgaca
     3541 tcgagaccct gggcatcgac gaaaacgaat tgatcgcgcg cacagccgaa ctcgaggacg
     3601 atgcgcggcg ccgtgtgcac cagctggtgc gcgggttcat cgcccgagtt ggtggctgag
     3661 cgcgtccgtg acgctgggct gcctgaaccg gtggcccagg tcgagcaggc ggtcgggcgt
     3721 cacccgctgg ttggcctcgg cgagttcctt gatgaaaggc accggcagca gcgccgggcg
     3781 atgcaacacc cccgccagcg ccttggtgta ctcgttgttc tgaacaggct gtggtgcaac
     3841 agcattcacc ggaccgacga gtctgtcgtc ccacaacgcc ggtgatagac gtccaccagg
     3901 tcatcgatgc cgatccacga cagccattgc cggccactgc cgagccggcc ccccagtccc
     3961 gcggcgaaca gcgggcgcag cagccgcaac gtcccgcccc tggcggcctg cacgatgccg
     4021 gtgcgcacgg cgacgacacg cagccccaca tcctcggcgg gagccgtcgc ggcctcccag
     4081 tccgcgacga cgtcggcgag aaatccgtca ccgcgcgcgc tgtgctcggt cagcacgtcg
     4141 tcgccgcggt cgaacccgta gtagccgatg gccgaggcgg tgacgaacac cgacggtccg
     4201 ccgtcggcgt cggccgcggc cttcgccagc cgccgcgtcg gctcgatccg gctgtcgcgg
     4261 atgtcccgct tgtgcgcgga ggtgaaccgc cccgcgatcg acgcgcccgc gaggtggatg
     4321 accgcgtcga cgccgctgag cagatcggcc gcggggttgg ccgggtccca gtatcgctcg
     4381 tcgccgccgc ggggaagccg tctgaccagc cggatcaccc ggtgaccggc actggtcaga
     4441 aacgccgaca gcgcagagcc gaccaggccc gaggcgccgc tgatcgcgac cactaatgcc
     4501 ccggccccgg cctcggccgc cctgcggtgt gcggccaggt cgtcggccag ctggcggtgc
     4561 cggtagacga acatcgaccg cagcagcgca gcgggcaccg gggtgtcgac gtggtcgcgc
     4621 accctggtct tgcccggagc ggcgtagctg aactcgtggg tgtgccgcca ccccccgatc
     4681 cggagctcgt cgacaaaccg gcggcccgcc acgaaacccg acgggtcgtg ccgggcgacc
     4741 cagcgcaggc ctgcaggcaa ccccaacacc gcgcgcccgt cggccagcga gtcggcttcc
     4801 gcaatcaccg tgagcggttg ccacggcggc accagccgcg gcatcgcgcc cggccgggca
     4861 tgccaatcga agacgtctga ttgcggctgc gggatcacac tcgagtactc gatgcccata
     4921 tcggggattc gatgccgatc ggtctgcgga ttggaggaca ctgggacggt atgcgagggg
     4981 gcacgttcgc cgaccgccgg gaggccggcc gggtcctcgc cgacgcactt gccgcctatc
     5041 gcggcgctcc cggcctgctg gtgctcgggt taccgcgcgg aggtgtgccc gtcgcgtggg
     5101 aggtggccgc cgcgctgcgc gcgccgctgg acgtcttcgt cgtccgcaag ctcggcgcgc
     5161 cgcgctggga ggaactggcc gtgggagcgc tcgcgagcgg cggcctggta gtcctcaacg
     5221 acgacgtggt gcgcagcctg cggatcagcg acgagcagct tcagcgggtg atcgacaagg
     5281 aagccgctga gttgtcccgt cgcgaggacg cctaccgcgg cgggcggcca ccgatcgaac
     5341 cggccggcaa gacggtgatc ctggtcgacg acggaatcgc cacaggggca agcatgctgg
     5401 cggcgacccg ggccatccgc gcggccggcc cggcgaaaat cgtggtcgcg gtcccggtgg
     5461 cgccgccgtc ggcgtgccgc cagctcgctg atgccgccga caacgtcgtg tgcgtccagg
     5521 tgcccccgtc gttcctggcg gtcggcgagt actaccgcga cttccgccag acctcagacg
     5581 acgaggtgcg agagctgctg gccaccccga cgcgctgagg tcagcgctcg gcgggattct
     5641 cttcggttga ctcggggtcc gccgactcgg tttggtccgc ctgctcgctg agcgagggct
     5701 gttcttcgtc gggatactcg gcgtcgaact cctcgtcgtc ccggtcatct tcgggcgact
     5761 cagccgcggg ggcggcgtcg cgtttctgcc gctcccgcaa cgcatcgacg atcaacagga
     5821 tcacgccgaa cacgctggcg ccgatgcaca cccacgcgat gatctcgttg ctggtgacca
     5881 ctgcggtcac gagcgcggcg aggccgatca cggccatcac cagtgcaacg atcagcatga
     5941 tcagccccgg ctagttgtta ccccggctga attggttgaa tgccgcgccc tcggcgttgg
     6001 cgctggaatc gacgggggcg gcggatccgc gctggcccag ctcttcgagc tgcgattcga
     6061 ggtaggtctt cagccgcgtg cggtattcac gctcgaacgt ccgcagctgc tcgaggcgtc
     6121 cctccagcac cgtgcgctgc tggttgatgg tgcccatgat ctcggtgtgc ttgcgctcgg
     6181 cgtcggcctg cagcgcgtcg gccttctcct gggcttggcg cagctgcgtc tccgaacgca
     6241 tctgcgcgtc gccgagcatc gcgtcggcac gctggcgcgc ctcggcgacc gtggtgtccg
     6301 cggtgtgccg cgcctcggac aggatcgagt cggcgttcgc ccgcgcgtcg gcgagcagct
     6361 tgtcggcctc ggccttggcg gtgccggtga gccggtcggc ggtgtcctgc gccagcgcca
     6421 gcactcgcgc ggccttcagg ggcgactctt cgtcgatggg acccgcgggc tccggctgcg
     6481 gtggcgcagg cggcggcgcc tcgtagacgg gctgctgtgg cggctcgggc ggctgctcgt
     6541 acatcggcat ggcctgactg gtagcgacgc cgctgccgga tcgtgcgccc gccagctcct
     6601 gatcgagctc tgcgacgcgc tgccgcagat cggtgttttc ctcgatcagt cgagtaagct
     6661 cggcttcaac aagatcgagg aatgcatcga cctcgtcctc gttgtagccg cgtttaccaa
     6721 taggcggctt gctgaacgcg acgttatgga cgtcggcggg agttagcggc attttctgcc
     6781 ccctcaagtt ctggacggtc gaagctgatc taggagtgta accgtggtcg tagcccggac
     6841 tgtaactggc gtccatcctg tcacatcaga cccgccggtt gcaggcgagc ataataaata
     6901 agaggttatt tcaatctcgt cggatcagtt tggcctacgc accagcgttg agtagcgaag
     6961 tccagccgat gaacaccacc agcagcagca ccatgatcga aaggtcgaac cggatcgaac
     7021 cgaccgtcag ctgagggatg aggcggcgca acaacttaac cggtgggtcg gtgagcgtca
     7081 tgatgatttc gagtatcacc accgtggccc ctttgggccg ccagtcccgg ctaaacgagc
     7141 gaatgaattc gatgaccact cgggcgatga gcagcagcca gaaaacgaac agcaccaagc
     7201 ccagaatttc gaacagcagc cgcaactggc ctgacctcac tagagacgta gacgacaacc
     7261 cccggggggc gctgccagcc taccgtcgca tcactggtag gcgtaaaagc cggcttcagc
     7321 gatgcggcgg cgctcctcgg cggtgacgtc gatgtcggcg ggcgacagca ggaacacctt
     7381 ggtggcgacc ttgtcgaacg agccgcgcag cgcgaaggcc agcccggcgg caaaatccac
     7441 cagccgcttg gcgtcggcgt tgtccatcgt gaccaggtcg atgatcaccg gcgtgccgtc
     7501 gcggaagcgc tcaccgatgg tgcgggcctc gctgtagtcc ttcggacgca gcgtggtgat
     7561 cttggacagc gggctgccct cctcgaacag catcgccatc cggcgcgggt ccatcgccaa
     7621 cgcgccgcgg gtggcgcccc gcaacgagcc gaaccgtgac gacggcggac gctcgtagtc
     7681 acgtggacgg aaccgcggtt cgtcgtagcc accgcggtaa ccgggtgggt agtcgtcggg
     7741 gtcgcgatcg tcgtattcgc gatcccggcc ctcgtagcgg cggccgtatc cgtcgtcggc
     7801 gaagcgatcg ccctgggacc ggcgggagta gccgcgtccg cgctcgtcgt cgtcgtagta
     7861 ctcgtcgtcg tagtcctcca tcggcgccat gccgaagtag gccttgacct tgtgcaacgt
     7921 actcatcggt gaccctttcg ggaggctcgt tcttgatgac tacttgatga agatgtgaca
     7981 ggagtgacta ctcagggaga cgttagcggt cgtggcccca acaacgcggt accgacacgc
     8041 acacaggtcg acccatgttt gacagcggcc tctaggtcgg cggacatccc cgccgacaat
     8101 cgggtcgcgt cgcgatgtct gctcagtacc cgcagatgtt cgttttctaa ctttgcgaac
     8161 gcctcatcgg ggttggagtc cagcggtgga atagccatca gcccgacgag cgacaggctg
     8221 tttgcgctgg cgaccgcgtc gcacacctcg tccaccaact ccggtctgcg cgcgtccaca
     8281 ccgccccgcg tcgggtcccc gtcgatgctg acctgtacga acacctcgag cggatcgcgg
     8341 cgctggccag ttcccaacgc ggcggcgacg gcgcggccca gcgcggcgac cagtcgcgtg
     8401 ctgtcgacgg aatgggcggc gtacgcccac ttggcgattg accgcgcctt gttccgctga
     8461 atgtgtccga ccatgtgcca gcgcagcgat tcgccgacga gcgtggccac ctcggcggat
     8521 ttcgcggcgg cttcttgttc gcgggattcg ccaacagaac ggcagccgag cttggacaaa
     8581 attgcgacat cggaagccgg gaagaacttc gtaacgggta gcagctcaat ttctgccgca
     8641 ttgcggccgg ctgcggcggc cgcatcggca attcggcgtt gcaccgcagc caaagaagcg
     8701 gccaattgct tttcgcgccg cttcaaccga tgctcttcgc gcaagcgctc atcgcaactc
     8761 catccagatc agcgaggcca accgaccggt cggcgcgtcg cggcggtggc tgaacaggct
     8821 cgggtcctcg acggtgcagc gcgggtcggc gtcgacggct gtgacgccca aatcggttag
     8881 ctgacgggcg attccggccc gcaggtccag cgccgcggtg ccttggcagc tggtggtccg
     8941 gctacccggc aacgccgtct cgacctcggc cgccatctcc tcggggacct cgtagttcgg
     9001 gccgctgacc gccgggccga gcagcaccga cacgtcgtcg gggtgcgcgc ccgcggcctg
     9061 catcgcttcc agcgtccgcg ccaccacgcc acgctgggcg ccgacccggc cggcgtgcac
     9121 cgccgccacc acgcccgcac gggcatcggc catcagcacc ggtacgcagt cggccgtgat
     9181 cacggcgagc gccagccgcg gcgtcgtcgt caccaacgcg tcggtgccct ctactggggt
     9241 ggtgcgcggg ccgtccaccc tctcgacccg gtcgccatgc acctggttca tccagatcac
     9301 gtggtcctcg tcgagcccgg ttgccgcggc caggcgcttg cggttggccg ccaccgcttc
     9361 cggctcgtcg ccgacgtggt caccgaggtt gaacgtgtcg aagggcggct tggagacgcc
     9421 gccggcgcga gtagtggtca cgcgccgaat acggtgcccg atgtcgccga ccctgcggct
     9481 ccgatcaccc acgcggtcca gtatctagcg gcgcatgaac ggcggcacgt ccacgtcgtc
     9541 gtcgtcatcg ccgccgatgc gcacgctcga accgttggtg tgcacgggca cgctggccgc
     9601 atcggccggc tcgaacagcg tcgacgtcac cgtgccagcg cttccggccg ccaccttggc
     9661 gcccacgccg accacccgct tgctgcgggc gctggtggca tcgaagccgg ccgcgatcac
     9721 ggtgacgcgc acctcgtcgc ccagcgagtc gtcgatgacc gtgccgaaga tgatgttcgc
     9781 gtcgggatgc gcggcctcct gcaccagcga cgcggcctcg ttgatctcga acaggcccag
     9841 gtcgctgccg ccggcgatcg acatcagcac gccctgcgcg ccttccatcg acgcctccag
     9901 cagcggcgag ttgatcgcga tctccgcggc cttcagcgac cggccgtcac cgcgtgccga
     9961 gccgatgccc atcagcgcgg tgccggcacc ggacatgatg ctcttgacgt cggcgaagtc
    10021 gacgttgatc agtccgggcg tggtgatcag atcggtgatg ccctgcacgc cgttgagcag
    10081 cacctcgtcg gcgctgcgga acgcgtccat cagcgatacc gcggcgtcac ccatctgcag
    10141 cagccggtcg ttggggatca cgatcagcgt gtcgcagctc tcgcgcaacg cgttgatgcc
    10201 ggcctcggcc tggtcgctgc ggcgcttgcc ctcgaacgag aacggccggg tgaccacccc
    10261 gatggtcagc gcgccgagct tgcgggcgat ggtggcgacg acgggcgcgc cgccggtgcc
    10321 ggtaccgcct ccctccccgg ccgtgacgaa caccatgtcg gcgccgcgga gcagctcctc
    10381 gatgtcatcc ttggcgtcct cggcggcctt gcgcccgacc tcggggtcgg cgccggcgcc
    10441 aagaccgcgg gtggagtcgc ggccgacgtc gagcttgacg tcggcatcgc tcatcaacag
    10501 cgcctgcgca tcggtgttga tggcaatgaa ctccacgcct ttgaggccct gctcgatcat
    10561 ccggttgacg gcgttcacgc cgccaccgcc gatgccgatg actttgatga cggcgaggta
    10621 gttgtgcgga ggggtcattg atcgccttcc tgctaaaacc ctcaacctca accataggct
    10681 tagagttatg tcaagtagtt ccgcgcaaac agaacggtat ggcgggctcg cacgggatta
    10741 acgcaggcgc gccgtgtcgt ttgcaggaat tttctatttg accgtgggga ggtccggact
    10801 ggacacgtca taggtgtggc ccggccgggt gagcagcgcc gccagcttct cggccttctc
    10861 gtcggtgcgg tcggtcgtcc cccagatcac cacacgtccg tccaccaacg tcaacgtgat
    10921 cgacgccacc gacggcgcgg cgatgcggcc gatttgcccg gctacctcgg gacgcagggc
    10981 ggtcagcacc tgaagcgcgg ccttggtggg cgggtcagtg gggccggggt tgtcgacgtc
    11041 gaggtacggc acgccgggcg ggggcggcgc ggtggcgaaa tcgacgccgt cgcggtcgaa
    11101 caggtgcgtg ccgtcgggca tgtccttgac cgccaccgga attcgctcgg ccacggtgac
    11161 ccgcagcgac gacgggtact cgcgctgcac ccgcaccgag gccacccggc tgatcgtcgc
    11221 gacccggttc gccaccttgt cggtgtcgac ctgcagcagc ggggtgccgg tgcgcaccgc
    11281 caccgcggcg acgacctcgt cgcgcgggat cgcctccagg ccctcgatct cgagattgcg
    11341 gaccgacatc accggagtga agtacagcac cagccccagg cccacggaga cgaccgtcag
    11401 gataaccgcc cacgtcagca ccttcagccc ccgaacaacg cctctgccaa cgggtttcgg
    11461 agcggtcgcc gacgcggtcg ccttccgctt ggcctcccgc cgggcctcct cgatcgcgcg
    11521 agcgcgcgca gcggcctcgc ggcgttgttc gcgttcgcgc cgggcgcgcc gccgcgggcc
    11581 ctccaactcc tccgactctt cgtcgggcgt gctgacctct ccgggcagcg gctctgtcat
    11641 ccgcgcatca cccccgagcg gccgggtgcg ctgcggttgg ccttgacctg caccgacgtc
    11701 agaatctccg gaccgagcat cgtgatgtcg ccggcgccca tcgtcacgac gacgtcgccc
    11761 ggtgccgtta cctcggccac ccggtcagcc accgcggaga agtcgggcac gtaggacacc
    11821 gggacgctga cgtgctcggc gaccagcgcg ccgctcaccc cggccatcgg ctgttcacgc
    11881 gcaccgtaga cgtcgagcac gaacaccagg tcggcgctgt tcagcgcggt gccgaagtca
    11941 cgtgcgaaaa gctctgtgcg cgaatacaag tggggctgga acacggcgat cgcacggccg
    12001 ctgcgatccg gcccttgctc gatgagggtg cgcaccgccg tcaacgtcgc gcgaatctcg
    12061 gtcggatggt gggcgtagtc gtcgtacacc cgcacggagt tcgccacgcc gaccagatcg
    12121 aaccggcgcc gcaccccctc gaacccggcc agcccgtcga ggacgcactc cggttccgcg
    12181 cccgcttcaa ccgcggccac cagcgagccc agcgcgttga gtgccatgtg gcggccgggc
    12241 accgacagcc gcatcacccg cggcagcgcc tcacccgcca gctgaacgtg cgcgaccgcg
    12301 ccggtgccct gcggctccca gctcaacagc cggccggcca tctcagctcg ctcggctggg
    12361 ctgtctgctc ttcgcccgag aggctcatcg ctgccgtacc gcaacaccct ggtaccccgc
    12421 gccgccgccc ggtcggccag cgccgccgcg cccgggtcgt cggtgcagac caccagcgcg
    12481 ccgccggggg ccacccggtc cgcgaagtcg tcgaataccg cggtgtaggc ctgcgcgctg
    12541 ccgaagaagt ccaggtggtc gacgtcgatg ttggtcacca ccgcgacgtc gggggcgtat
    12601 tcaagcagcg agccgtcgct ttcgtcggcc tccgcgacga agtagtcgcc gctgccgtgg
    12661 tgcgcgttcg tgccggcctc cccgagatcg cccccgaccg cgaacgacgg gtcgagaccg
    12721 cagtgctgca gcgcgacgat cagcatcgac gtcgtcgagg tcttgccgtg cgtgccggtg
    12781 accgtcaacg tgcggtagcc ggtcatcagc atcgccagca ccctcgggcg cagcacgacc
    12841 gggacgccgc gacgccgggc ctcgacgagc tcagggttgt tcttcgggat cgctgcgcgc
    12901 gtggtgatca cgacggtcgg gccgccgggc agcaggtcca gcgacgacgg gtcgtggccg
    12961 atgcgcacca cggccccacg ggcgcgcaac gcgagcaccc cgcgcgactc cttggcgtcg
    13021 gaacccgaca cctgcgcgcc gcggtcgagc aggatgcggg cgatgccgga catgcccgcg
    13081 ccgccgatgc cgaccatgtg cacccgcgcc agctcgggcg gcagcggctg cggggtcacc
    13141 gccgcctccg ggcttgccgt gcgacgtcca gcgccacctg
//
LOCUS       DASWBF010000503         5972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_786275,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000503 DASWBF010000000
VERSION     DASWBF010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786275"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VF874_18480"
     CDS             complement(<1..348)
                     /locus_tag="VF874_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate transport system regulatory protein
                     PhoU"
                     /protein_id="HEX9177969.1"
                     /translation="MGTVEQLPDACLHAGAQMLRATKALILADAGEAQDVLRVHVDLT
                     VRIVEAEASVLQLLRHEGDPRDLAVSLQTVADVNQMDVLARRVARVAMHAHPMRALPD
                     DVNGYFSDMGDRAD"
     gene            complement(667..1767)
                     /locus_tag="VF874_18485"
     CDS             complement(667..1767)
                     /locus_tag="VF874_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9177970.1"
                     /translation="MTAAAMPVTDDQRAQLERMASSTSLPHRQVVQARGLLWACDGVA
                     NEEIARHCGVDSDTVRRWRSRFAEQGVAGVGRIAKGRGRKPSLPPGTVEEVLRLTHKE
                     KRVDGSTHWSTRTLAARVGIGKDAVAKIWRDHNLKPWKVETFKVSNDPRFEEKLVDVV
                     GLYLNPPARAAVFCFDEKTQCQALDRTQPSLPMKPGRAGTMTHDYKRNGTIDLFAAMN
                     VATGEVLTDLRKGHAGPDVLRFFKQIDAAVPRGLGVHVILDNLSAHSTPEIAKWLAHK
                     DRRRWHLHYTPTSSSWLNLVERWFKELTDKRLRRGTFASIAELTEAITTWATHWNLDP
                     KPFIWKATADDIIAKVRRGRATLHQIKSQTDH"
     gene            complement(2261..2500)
                     /locus_tag="VF874_18490"
     CDS             complement(2261..2500)
                     /locus_tag="VF874_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177971.1"
                     /translation="MRKLRSEMHRRMLGNGYCARPVELDCHFESICESCSFFVTTIEF
                     RPTLQRQRDDAARKGQIGRQKIFDGLLNRLDTDAS"
     gene            2700..2930
                     /locus_tag="VF874_18495"
                     /pseudo
     CDS             2700..2930
                     /locus_tag="VF874_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854288.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
     gene            complement(2896..4008)
                     /locus_tag="VF874_18500"
     CDS             complement(2896..4008)
                     /locus_tag="VF874_18500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9177972.1"
                     /translation="MRACAAVGVVVTHVAFQTGHSSGVAGRLFGRFDLAVAVFFALSG
                     FLLWRGHAAATRGLRHIPPTGHYLRSRIVRIMPGYLVAVVVILTLLPDAETSSLTVWL
                     ANLTLTQIYVPLTLTAGLTQMWSLSVEVSFYLALPLLALLARRLPVRARMPAIAAVGV
                     ASLGWALVPLHTPEGVNLLNWPPAFFSWFAAGMLLAEWTVSPLGWMHRLARRRVLMAV
                     IAVGAFLVAASPLAGPEGLIPGTVGQFVVKTAMGAVLAAALIAPLVLDRPDTPHRFLG
                     SRPMVTLGRWSYGLFVWHLAALVMVFPVIGEFPFSGHMPVVLVLAVIFGFAIAAVSYA
                     LVESPCRVALRRWEYRRTAPPLDSAVTDAAKLAVAR"
     gene            4292..5536
                     /locus_tag="VF874_18505"
     CDS             4292..5536
                     /locus_tag="VF874_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3068 domain-containing protein"
                     /protein_id="HEX9177973.1"
                     /translation="MNRVLALRTTANAILFLGAALIMAAILLSTYTASRVTRIPLDID
                     MTLQSSGTAAKALDPASLSTDRFVANNDVPVVQQQQFSTESGVDTPSNADVVTLQVGT
                     TLRRTDKQQDSGLLLAIVDTVTLNRMTAMAVASENDTAGFLQRPRTMDDTSPPTPMKV
                     GHEGLNYRFPFDVEKKTYPYFDPIAQKTFDANYDGEEDVNGLTTFHFKQNVGLDNNGK
                     LVEPVEYPSLYLDKEDATVTARAALWGLPGPPDDQVKMKRYYAAQRDFWVDPVTGTIV
                     KTEERAMHYFVRCDDPECKTRDPLKPDVTLVDYTVASTQATVEQQVNAARDERDRIAV
                     WARILPITFTALGIVALVGGALLALFTLRTDTALIDPGLDDAEHGFFGPRGPSGEPVP
                     GAEAETEKLPAQRRPPPAPPPP"
     gene            5566..>5972
                     /locus_tag="VF874_18510"
     CDS             5566..>5972
                     /locus_tag="VF874_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177974.1"
                     /translation="MAPAYALTLALAITAPLLAPGHLLLRDAVSTPRSYLSDDALGLA
                     ESAPRALPQDFLLASASWLIDGGIVVKALLVVGLWLAGWGAARLVALVIPDAGLGGEL
                     VAVTVAVWNPYVAERLSQGHWSLLVGYGCLPWVA"
BASE COUNT         1020 a         1951 c         1958 g         1043 t
ORIGIN      
        1 gtctgcccgg tcgcccatat cgctgaagta gccgtttacg tcgtcgggca gtgcgcgcat
       61 ggggtgtgcg tgcatggcga ctctcgcgac ccgccgtgcg agaacgtcca tctggttcac
      121 gtcggcgacg gtttggagtg acacggcgag gtcgcgcggg tcaccctcgt gccgcagcag
      181 ctgcaatacg cttgcctccg cctcaacaat ccggacggtc aagtcgacgt ggaccctgag
      241 gacatcctgc gcttcacccg cgtccgcgag aatcaacgcc ttcgtggcac gcaacatctg
      301 ggctccggcg tggaggcagg catccgggag ctgctcgacg gttcccattc agcaaggcta
      361 aacccaccgg cgaacgcagt ggcgtggcgg ttgggctatt gacgtctcaa tttttccccg
      421 cgtcgaatgg accagacctc gtggccaccc ctcccatcct caagggccac ctcgtcgccg
      481 tcgaggagta tcgggatacc tcccgttgga ttaggtaggt gcgaaaaagc atgcgtgatc
      541 ttgtctctga agcccgcaaa gccgtcgtcg ctgtctgcga gctcaaagcc cgatgccatt
      601 tgtagggctc tcccttcgtg aacgcgccag cgattgttct ctacggtgtg ccgacctaat
      661 cacgtcctag tggtctgtct gcgatttgat ttggtggagg gtggcgcgtc cgcgtcggac
      721 cttggcgatg atgtcgtcgg cggtggcttt ccagatgaat ggcttggggt cgaggttcca
      781 gtgggtggcc caggtggtga tcgcctcggt gagttcggcg atgctggcga aggttccacg
      841 ccgcaggcgt ttgtcggtga gttccttgaa ccaccgttcg acgaggttta accatgagct
      901 ggaggtcggc gtgtagtgca ggtgccagcg gcggcggtcc ttgtgcgcca accatttggc
      961 gatctcgggt gtggaatgcg ccgagaggtt gtcgaggatg acgtgtacgc ctagaccgcg
     1021 cgggaccgcg gcgtcgatct gtttgaagaa cctcagaaca tccggaccgg cgtgtccttt
     1081 gcgcaggtcg gtgaggactt cgccggtggc cacgttcatc gccgcgaaca aatcgatggt
     1141 gccgttgcgt ttgtagtcgt gggtcatcgt cccggcacga cccggcttca tcggcagcga
     1201 cggctgggtg cggtccagcg cctggcactg ggtcttctcg tcgaagcaga acaccgccgc
     1261 gcgcgcgggt ggattcagat acagcccgac aacatcgacg agcttctcct cgaaccgcgg
     1321 atcgttgctg accttgaacg tctcgacctt ccacggtttg aggttgtggt cacgccagat
     1381 cttggccacg gcgtccttac cgatcccgac ccgtgcggcc aaagtccttg tgctccaatg
     1441 ggtggaaccg tcgacccgct tctccttatg cgtgagccgc aacacctctt cgaccgtgcc
     1501 cggcggcagg ctcggcttac gcccgcgccc cttcgcgatc cgtccgaccc cggcgacgcc
     1561 ctgctcggcg aaccgggaac gccagcgccg caccgtgtcc gaatccaccc cgcagtggcg
     1621 ggcgatctcc tcgttggcca ccccgtcgca cgcccacaac aacccccgag cctgcaccac
     1681 ttggcgatgc ggcaacgacg tcgaggacgc catccgttcc agctgcgcac gctgatcgtc
     1741 ggtcaccggc atcgccgccg cagtcatgac aggcataaga cagactacca cattatttca
     1801 cagacacacc actatcctca gccaccacgg gcgaaggtca ccatcctgca caccgatcgg
     1861 agtcacaacg atcccgcctg acgcgccaac gtcgctgatg atccacgcga gtgctgctat
     1921 ggcgttctgc ttgagcttgg aagtggtgta ccggcgacac tcgatgatga cgatcgcccc
     1981 atcctcggtt ttgacgggga ggagcctgac ccgattccac ggacagtggt tgtgtggcaa
     2041 ttgattgtgt cctaaccgct ggcagtatgg ctagtcgtgg cgaggacgtg gttgaagcat
     2101 ccccggtttc atgcacacct cgttacgtgt gaccggtgtg ctttccggcg tgcagtcgag
     2161 cgtagtactc ggcctcggcc tccgcaggcg ggcgtcgccc gaaccggtgc atcagcctgc
     2221 gctcgttgta cttatgcggg tgatcctgtc aagtgggatg tcaggaggcg tcggtgtcga
     2281 gccggttgag caggccgtcg aagatcttct ggcgtccgat ctggcccttg cgggccgcgt
     2341 cgtcgcgctg tcgttgcagg gtcggccgga actcgatggt ggtgacgaag aagctgcacg
     2401 attcgcagat cgactcgaag tggcagtcga gttcgacggg tcgcgcgcag tagccgttgc
     2461 cgagcatgcg gcggtgcatt tcgctgcgga gtttccgcat ctgtcggccc tcctcgtcag
     2521 ctggtagctg ggcgggttgg tgggtgtcgt agagggcttc cacccttttc ggtgacgttg
     2581 aagtactcgt cggcgacggt gcggtcggcg atgcgggcat acaacatcgt catcgacagc
     2641 gatcgatgtt tgatatgcgg gtgatctgtg gccgttcggc ctaggttggt tggtcgaccc
     2701 ccgacgacga tctcggccag gtgccgcacg cgatcgtcca caccgtcgac ggctcgcgcc
     2761 tcgacgaggc atcggtgatg gcattcgtcg gcgaacgtcg cgccccctac aagctccccc
     2821 ggacggttga attcgtcgac atgccactgc gcgacgacgc cggcaaggcg cggcgctcag
     2881 cggtgcgggc cgacgtcatc gcgcgacggc aagcttggct gcgtcggtga ccgcactgtc
     2941 cagcggcggc gcagttcggc gatactccca gcgccgcaac gcaacccggc acggcgactc
     3001 gaccagcgcg tagctcaccg cggcgatcgc gaacccgaag atcacggcca gcaccagcac
     3061 caccggcatg tggccgctga acgggaactc gccgatgacc gggaacacca tcaccaacgc
     3121 cgccagatgc cagacgaaca gcccgtagga ccagcgaccc agtgtgacca tcgggcggct
     3181 gccgagaaac cggtgcgggg tgtcgggacg atccagcacc agcggtgcta ttagggcggc
     3241 ggccagcacc gctcccatcg cggtcttgac cacgaactgg ccgaccgtgc cagggatcag
     3301 tccctccgga ccggccagcg gcgacgctgc gacgaggaat gccccgaccg cgatcaccgc
     3361 catcagtacg cggcgtcgcg ccagccggtg catccagccg agcgggctga ccgtccactc
     3421 cgcgagcagc atcccggcgg cgaaccacga gaagaaggcc ggtggccagt tcagcaggtt
     3481 gacgccttcg ggggtgtgca gaggcaccag cgcccaaccc aagctggcca cccccaccgc
     3541 ggcgatcgcc ggcatccgcg ccctgaccgg gagccggcgc gccagcagcg ccaacaacgg
     3601 cagcgcgagg tagaagctga cctcgaccga cagactccac atctgcgtca acccggcggt
     3661 cagtgtcagc ggcacgtaga tctgggtcaa tgtcaggttc gccaaccaga ctgtgagact
     3721 tgatgtttcg gcgtcaggaa gcagcgtcag gatcaccacc acggccacca ggtatccggg
     3781 catgatccgg acgatccgcg agcgcaggta gtgtccggtc ggcggaatgt ggcgcaggcc
     3841 ccgcgtcgcg gcggcgtgtc cgcgccacag cagaaagccg gacagcgcga agaacaccgc
     3901 gaccgccagg tcgaaacggc cgaacaagcg cccggcgact ccggacgaat gtccggtctg
     3961 aaacgcgacg tgggtgacga cgaccccgac agccgcgcat gcccgcatcc cctccaccgc
     4021 gggcaggaag ttgcccgtgc cgccgcgcga tgtgggagca ctcccccgcg agcgggaggt
     4081 acccccaccc gcttgcgggg gaccgcttgc gcgaagagca gaaggcactg tcacgccgat
     4141 cagtctgcac ggccagggga aagtcgccgg tcatcgggta gtgccgcggt cgtcgttcac
     4201 cttcgtttct gctgttaggg tcaaacgggt ttgcctccac tgactgggtc ggggccagtg
     4261 gcggcgggaa gtcatgggat gggaggcggg tttgaaccgc gtacttgcgc ttcgtacgac
     4321 ggcaaacgcg atcttgttcc tcggcgcggc actgatcatg gcggcgatac tgctgtccac
     4381 ctacacggcc agccgagtca ccaggatccc gctcgacatc gacatgacgc tgcagagcag
     4441 cggcaccgcg gcgaaggcct tggatccggc gtcgctgtcg acggaccgat tcgtcgccaa
     4501 caacgacgtg cccgtggttc agcagcagca gttcagcacc gagtcgggtg tcgacactcc
     4561 gtcgaacgcc gacgtcgtca cgctgcaggt gggcacgacg ctgcggcgca ccgacaaaca
     4621 gcaagacagc ggcctgctgc tggccatcgt cgacacggta acgctgaacc gcatgaccgc
     4681 gatggcggtg gccagcgaaa acgacaccgc gggcttcctg cagaggccgc ggacgatgga
     4741 cgacaccagc ccgcccacgc cgatgaaagt cggacatgag gggctgaact accggtttcc
     4801 gtttgacgtc gagaaaaaga cctatccgta cttcgacccg atcgcgcaga agacgttcga
     4861 cgccaactac gacggcgaag aggacgtcaa cggactgacc accttccact tcaagcagaa
     4921 cgtgggcctg gacaacaacg gcaagctcgt ggaaccggtt gagtatccgt cgctgtacct
     4981 cgataaggag gacgccaccg tcaccgcccg cgcggcgctg tggggtctgc ccggcccgcc
     5041 cgacgatcag gtcaaaatga agcggtacta cgccgcccag cgcgacttct gggtcgaccc
     5101 ggtcaccggc acgatcgtca agaccgagga gcgcgcgatg cactacttcg tgcgctgcga
     5161 tgatcccgag tgcaaaaccc gcgaccccct caagccggac gtcacgttgg tcgactacac
     5221 cgtggcgtcg acccaggcca ccgtggaaca gcaggtcaac gccgcgcgcg acgaacggga
     5281 tcggatcgcc gtctgggccc gcatcttgcc gatcacgttc accgcgttgg gaatcgtcgc
     5341 acttgtcggc ggcgccttgc tcgcgctgtt cacgctgcgg accgacaccg cgttgatcga
     5401 tcccggcctc gacgacgccg agcacggctt cttcggtccg cgcggaccgt cgggtgaacc
     5461 cgttcccggg gccgaggccg agacggagaa gctgccggcg cagcgtcggc cgccgccggc
     5521 gccaccgcct ccgtagcaga ggtcgtgtca ctggcgcggc gctgcctggc gcctgcatac
     5581 gcgctgacct tggcgctggc gatcaccgcg ccgctgttgg cgcccggtca tctgctactg
     5641 cgggatgcgg tgtcgacgcc gcgttcctac ctgtccgacg atgcgctggg gctggccgag
     5701 tcggcgcctc gcgcgctgcc gcaggacttc ttgctcgcgt cggcatcgtg gttgatcgac
     5761 ggcgggatcg tggtcaaggc cctgctcgtg gtcgggttgt ggctcgccgg ctggggcgcg
     5821 gcgcggctgg tcgcgctggt gatccctgac gccgggctcg gcggggaact cgtcgcggtg
     5881 accgtcgcgg tctggaatcc atatgtagca gaacggcttt cgcaagggca ttggagtctg
     5941 ctggtcggct acggatgtct gccgtgggtc gc
//
LOCUS       DASWBF010000504         6784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1035651,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000504 DASWBF010000000
VERSION     DASWBF010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035651"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..463)
                     /locus_tag="VF874_18515"
     CDS             complement(182..463)
                     /locus_tag="VF874_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9177975.1"
                     /translation="MTHTPNLPVPAGATADDWSDFTDIDGDHARALLWSRHGTDKIGV
                     TVDGWQTADGQVIRAVSLYDADKELTAADARKLVAALLNAADELDRLDG"
     gene            complement(571..1881)
                     /locus_tag="VF874_18520"
     CDS             complement(571..1881)
                     /locus_tag="VF874_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9177976.1"
                     /translation="MFSETRYALNGDLHVAYRTSREGPRDIVFVPNWFTCCEVLPELP
                     SIQGWVEAMTSLGRLIFFDQPGTGASDPVTPDALPTLEQWADSIGAVLDDLGSNEAVL
                     LAVDGAFAPAALFAATHPSRTTALVVLDGYADAGGTPLVQDETPAAMVRMWGSGKIQH
                     LVNPDMPWNEEIRATWARQERLAASPRTLALVITLATDVDVRAVLPTVRVPTLVVQHS
                     DDVIIPPAKGKYIAEHIPDAKYVELPGRNWYHIVEPWRPSFKEIHKFLTGQQAEVVDD
                     RVLATVLFTDIVDSTRRAAEIGDRDWHALLDAHDAVVRVQLARFRGREVNTSGDGFLA
                     MFDGPQRAIRCAMAIRDAVQALGIQVRAGLHTGECEVRGDDIGGIGVHIGARVSALAG
                     PNDVLVSSTLRDLVIGSGLKFEERGAHQLKGVPGEWHLFAVASQ"
     gene            complement(2043..3233)
                     /locus_tag="VF874_18525"
     CDS             complement(2043..3233)
                     /locus_tag="VF874_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9177977.1"
                     /translation="MRTGRVAAPPTRDRRARVAVAALFLTNGALFANLLPRYPEIKAD
                     LGLSNAAYGVAVAAFSVGALVTGPLAASLIRRFRSARVAVFGSAGIAVFAWLAGVAPS
                     VVTFAAALFVAGGADAVTDVAQNAHGLRVQRRYARSIINSFHAVWSAGAILGGAMGAV
                     AIALHVPRGTHLAVSAVVFSAVCVTAYRYLLPGPDEAAGALRQAPTALGGAPVATWAA
                     LAALVTIAIAAATVEDAGSSWATLYMRELGAPGAIAVSGYIAAVGSQLIGRVLGDRLV
                     DRFGQRAVVRTGGVIAAVGMGAALAFPSMPTVIGGFAAAGFGVATLIPAAMQRADEFP
                     GLKPGTGLTAVTWLMRVGFVFAPMIVGAIADAFSLRAGLLTVPVAGIVVIALAGVLAA
                     RRKR"
     gene            3276..5414
                     /gene="malQ"
                     /locus_tag="VF874_18530"
     CDS             3276..5414
                     /gene="malQ"
                     /locus_tag="VF874_18530"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX9177978.1"
                     /translation="MTLQQQSLAELAGRYRVATQYYDANGHPMVVPDNTLVAVLKALG
                     VAADTEEERLAALRNHDRAYWSRSLPPVIVMRAGTPAPFWVHVTHGDPVRLWIRVEDG
                     TVWAGLRQLDNFTAPYELDGRLVGEATFELPANLPLGYHRLHLDDANSATGTAVIVTP
                     ANLTVPERLGSRRTWGLATQLYSVRSKSSWGIGDLTDLTDLAIWAGSRHRAGYVLVNP
                     LHAAAPTAPMEPSPYLPTSRRFINPIYLRVEAVPEFAFVRRRGRIRRLREDVQSGAKR
                     VDSIDRDAAWAAKRAALKELHRVPRSAGRDLAYAEYRQREGRALDDFATWCALAETYG
                     SDWHHWPQDLQRPDAPGIAAFAEEQANEIDFHRWLQWQLDDQLIVAQAKALATGMALG
                     IMHDLAVGVHPNGSDAWALQDVLALGVSVGVPPDAFNQQGQDWSQPPWRPDALAEQEY
                     RPFRSMVNAVLRQSGGVRIDHVMGLFRLWWIPKGARPTEGTYVHYDHEAMVGILALEA
                     QRAGALVVGEDLGTVEPWVRDYLHDRGVFGTSILWFEMDRDGAGEPLPAERWRECCLS
                     SVTTHDLPPTAGYLAGEHVRLRDRLGLLTRPFDEELAGDRAEQEAWLTELRRAGVLAD
                     DPDVDDTVLALHRYLGRTPSKLLALSLSDAVGDRRTQNQPGTSDEYPNWRVPLSGPKG
                     RRLLLEDVFRDHRAATLADAMRAATGGDAQ"
     gene            5411..6590
                     /locus_tag="VF874_18535"
                     /pseudo
     CDS             5411..6590
                     /locus_tag="VF874_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932514.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
BASE COUNT         1177 a         2363 c         2201 g         1043 t
ORIGIN      
        1 acccgaccag ccactacaac gggccgtaca tgctgcgtca ccgggccgca ccccaagagg
       61 tgtggatggt gttgggcacc tacaacacgt tcgaggctgt gaagtcggca gccgatctac
      121 acgaacatgc gatgagccaa tgacacagag cactcttcag ttggcgtaag tcgtccatca
      181 gtcagccgtc gagccggtcg agttcgtcgg cggcgtttag cagcgcggcg accagcttgc
      241 gcgcgtcggc tgcggtgagt tctttgtctg cgtcatacag cgacacggcg cggatgactt
      301 gcccgtctgc ggtctgccag ccgtccacag tgaccccgat cttgtcggtg ccgtggcgcg
      361 accacagcag ggcgcgggcg tggtcgccgt cgatgtcggt gaagtcggac cagtcgtcgg
      421 cggtggcgcc ggccgggacg ggaaggttgg gtgtgtgggt catgggcggg cctttcatct
      481 cgggaacgtg aactgttctt tagcgttctg gtgcgaccgg gtgcacaagc gcttacggca
      541 aggcgaaatg agtttcgcca cagccccccg ttactgagag gcgacggcga agagatgcca
      601 ttcgccgggc acgcccttga gttggtgagc gccgcgctcc tcgaacttga gccctgatcc
      661 gatcaccagg tcgcgcaggg tgctggacac cagcacgtcg tttggcccgg ccagggcgct
      721 cacgcgcgcc ccgatgtgca cgccgatccc accgatgtca tcgccgcgga cctcgcactc
      781 gccggtgtgc aaaccggcgc gcacctggat gccgagcgcc tgcaccgcgt cgcggatcgc
      841 catcgcgcag cggatcgctc gctgcggtcc gtcgaacatc gcgaggaaac cgtctcccga
      901 tgtgttcacc tcgcggcccc gaaaccgagc gagttgcacc cggacgacgg cgtcgtgcgc
      961 atcaagcaag gcatgccagt cacggtcgcc aatctccgcc gcgcgccgtg ttgagtccac
     1021 gatgtcggtg aagagcaccg tggcgagaac acggtcgtcg actacttcgg cctgctggcc
     1081 agtgaggaac ttgtggattt ctttgaagga cgggcgccag ggttcaacga tgtggtacca
     1141 gttgcggccc ggcagctcaa cgtatttcgc gtcgggtatg tgctcggcga tgtacttgcc
     1201 cttcgcgggc gggatgatca cgtcgtcgga gtgctggacg acgagggtcg gcacacggac
     1261 tgtcggaagg accgcccgca cgtccacatc cgtcgcgaga gttatcacga gagctagcgt
     1321 ccttgggctg gccgctagac gttcttgtcg tgcccaggtt gcccggatct cctcgttcca
     1381 cggcatgtcc ggatttacca gatgttggat cttcccgctg ccccacatgc ggaccatcgc
     1441 agctggggtt tcgtcttgaa cgagtggggt gccgcccgca tctgcgtaac cgtcgagcac
     1501 gaccagcgcg gtggtgcgag acggatgtgt tgccgcgaac agcgcagcgg gcgcgaacgc
     1561 gccgtcgacc gcgaggagaa ctgcttcatt gctcccgaga tcgtcgagca ccgcgccgat
     1621 gctgtcggcc cattgctcca aggttggcag cgcgtccggt gtgacgggat cggatgctcc
     1681 cgttcccggc tggtcgaaga agatgagtcg cccgagcgat gtcatcgcct cgacccaacc
     1741 ctgaattgaa ggcagctccg gaagaacctc gcagcacgtg aaccagttcg ggacgaacac
     1801 gatgtctcgc ggtccttcgc gggacgtgcg ataggcgacg tgcaagtccc cgttcagcgc
     1861 gtagcgcgtc tccgagaaca ttgcggaagt gtaagccgag gagcggctcg agtttcggtg
     1921 tcatgcaacc agctgaaaca aagtgtcctt cgcgtattcg gtgtcaacgc cgcaccaaca
     1981 ccaactttca tccggaacgc atgctgccgg acgacgagga cgacgaatcg gccagccact
     2041 agctagcgct tgcgcctggc ggcgagcact cccgccagcg cgattaccac gatgccggcc
     2101 accggcaccg tcaatagccc ggcacgcagg ctgaacgcgt cagcgatcgc cccgacaatc
     2161 atcggcgcga agacgaaccc gacgcgcatc agccaggtga ccgccgtcag cccagtgccg
     2221 ggtttcaggc cgggaaactc gtcggcgcgc tgcatcgcgg ccgggatgag cgtcgccaca
     2281 ccgaaccccg cggcggcgaa ccccccgatc accgtgggca tgctcggaaa cgccaacgcc
     2341 gctcccatgc cgacagccgc aatcaccccg cccgttcgga cgacggcgcg ctgtccgaat
     2401 cggtcgacga gccgatcgcc gagcacccgt ccgatcaatt gagacccgac cgcggcgatg
     2461 tatccggaca ccgcaatcgc gccgggcgcg cccaattccc gcatgtacaa cgtcgcccat
     2521 gaactgcccg cgtcttcgac ggtcgcggcg gcgatcgcga tcgtcaccag cgcagccagc
     2581 gccgcccacg tcgccaccgg cgcgccaccc agtgccgtcg gcgcctgccg gagcgccccg
     2641 gcggcctcgt cgggtccggg cagcaggtac cggtacgcgg tcacgcaaac agcggagaac
     2701 accaccgccg acaccgccaa atgggtgccg cgcggaacgt gcaacgcgat cgcaacggca
     2761 cccatcgccc cgccgagaat cgcgccggcc gaccacaccg cgtgaaacga gttgatgatc
     2821 gaccgcgcgt accgccgctg cacgcgcagc ccgtgggcat tctgcgcgac atcggtcacc
     2881 gcgtccgcgc ccccggcgac gaacagcgcc gcggcgaacg tcacgaccga cggcgccacc
     2941 ccggcgagcc aggcgaacac cgcaatgccc gccgacccga agaccgcgac ccgcgcggac
     3001 cggaaccggc gaatcaacga cgccgccagc gggccggtga ccagagcccc gaccgagaat
     3061 gccgcgaccg caacaccata ggcggcgttc gacagtccga ggtcggcctt gatctccgga
     3121 tagcgcggca gcaagttcgc gaacagtgcg ccgttggtca gaaacaatgc ggcgaccgcg
     3181 acccgggccc gacgatcgcg cgtcggcggc gctgcgaccc gtccggtccg catctgctgc
     3241 agactacccg cagggattcc gcaaagatgg ccaccatgac cttgcagcag cagtcactgg
     3301 cggagctcgc cgggcgttat cgcgttgcta cccagtacta cgacgccaac ggccacccga
     3361 tggtcgtgcc ggacaacacg ctcgtcgccg ttctcaaggc gttgggcgtc gccgcggaca
     3421 ccgaagaaga gcgcctcgcc gcgctgcgca accacgaccg cgcctactgg tctcgctcgc
     3481 tgccgcccgt gatcgtcatg cgcgccggta cccccgcgcc cttctgggtg cacgtgaccc
     3541 acggcgatcc ggtccggctg tggattcgcg tggaggacgg caccgtctgg gccgggctac
     3601 gccagttgga caacttcacc gccccatatg agctcgacgg gcgcctcgtc ggcgaggcca
     3661 cgttcgagct gcccgcgaac ctgccgctgg gttatcaccg gctgcatctc gacgacgcga
     3721 actccgcgac gggcaccgcg gtgatcgtca caccggccaa cctgaccgtt ccggagcgcc
     3781 tcggatcccg ccggacctgg gggctggcga cgcagctcta cagcgtgcgc tcgaaaagct
     3841 cttggggtat aggggatctc accgatctga cggacttggc catctgggcc gggtcgcggc
     3901 acagggccgg ctacgtgctg gtgaatccgc tccacgccgc cgcgccgacc gcgccgatgg
     3961 aaccgtcgcc gtacttgccg acctcacgtc gcttcatcaa cccgatctac ctccgcgtgg
     4021 aggccgtccc ggagttcgcc ttcgtccgcc gccgtggacg gatccgcagg ttacgcgagg
     4081 acgtgcagag cggagccaaa cgtgtcgact cgatcgaccg ggacgcggcg tgggcggcca
     4141 agcgtgcagc gctcaaggag ctgcatcggg tgccgcggtc ggcgggtcgc gacctcgcgt
     4201 atgccgaata ccggcagcgc gaggggcgtg cgctcgacga cttcgccacg tggtgtgcgc
     4261 tcgccgaaac atacggcagc gactggcacc actggccgca ggatctccag cgcccggacg
     4321 ctcccggaat cgccgcgttc gccgaagagc aggcaaacga gatcgacttc caccgctggc
     4381 tgcagtggca actcgacgac cagctcatcg tcgcgcaggc caaggcgctg gcgaccggga
     4441 tggcgctggg catcatgcac gacctcgcgg tcggcgtgca cccgaacggc tccgacgcgt
     4501 gggcactgca ggacgtgctg gctctgggtg tcagcgtcgg ggtgccgccg gacgcgttca
     4561 accagcaggg ccaggactgg tcgcagccgc cgtggcgccc ggacgcgctg gccgagcagg
     4621 agtaccggcc gttccgctcg atggtcaacg cggtgcttcg gcagtcgggc ggcgtgcgca
     4681 tcgaccacgt catgggactg ttccggctgt ggtggattcc caagggcgcc agaccaactg
     4741 agggcaccta cgtccactac gaccacgagg cgatggtggg catcctcgca ttggaggcgc
     4801 agcgggcggg cgcgctcgta gtcggtgaag acctcggcac cgtcgagccg tgggtgcgcg
     4861 actaccttca cgatcgcggc gtgttcggca cctcgatcct gtggttcgag atggaccgcg
     4921 acggtgccgg cgaaccactt ccggccgagc ggtggcggga gtgctgcctg tcctcggtca
     4981 ccacccacga ccttcctccg acggccggtt atctcgcggg cgagcatgtc cggctgcgtg
     5041 accggctcgg cctgctcacc cgcccgttcg acgaggagct cgcgggtgat cgcgccgagc
     5101 aggaggcgtg gctgacggaa cttcgccggg ccggcgtgct tgccgacgat cccgatgtcg
     5161 acgacacggt gctggcactg caccgctatc tgggccggac gccgtccaaa ctgctggcac
     5221 tgtcactgag cgacgcggtc ggggaccggc gcacccagaa ccagccgggc accagcgacg
     5281 agtacccgaa ctggcgcgtg ccgctgtcgg ggccgaaggg acgtcgactg ctgctcgagg
     5341 acgtgtttcg tgaccaccgc gccgcgaccc tggccgacgc gatgcgggca gcaaccggcg
     5401 gagacgcgca gtgacgtccc cggtggtgat cgacgacttg gcgtcgccga aactgagtcc
     5461 gctgcaacag cagatcctcg accacttcga gaagcggccc gtcaatctcg ggccggaaca
     5521 actgatcgcc gacgctgtcg ctcggaccgg cctcaccgac ttcgggcccc cggatttccg
     5581 ctcgcgcctc gacgcgcacg tcgcagctat cgacgccgac acgggcagca ccaacctcaa
     5641 ccggctgacg ttgcgcaacc gcatcgtccg gctgctgtca agtcggctgc tgctgaccga
     5701 tctgctgaac cgctaccccg aaatacatga cgtcgagatt gagaggccaa tcatcgttgt
     5761 cgggttaccc cgttccggca caacgcattt agttaacctg atcgccgccg atccgggccg
     5821 cagggcactg ccctactggg aaagccaaga gcctttcccg ttgcgcggcg agggacccga
     5881 cgtgaacggt gtcgaccccc gatacgcccg ctgcgtcgcc gagcacgaga tggtccagca
     5941 gacagcgcct ttcacccagg ccatgcacga ccggttcccg gcggccatcg aggaggaggt
     6001 cgagctcctc gacctcgact tcgccagcta cgtcctggag tggcacgcgc gggtgccgcc
     6061 gtggcgcgac tactacctga gcctggatca gcgtacgcat tacggctacc tgtgcaccgt
     6121 gctcaaggcg ctgaccttcc tgcgcgggcc acgccagtgg gtgctcaagt cgccgcagca
     6181 ctgcgagcag ctcggcccgc tgatcgacac attcccggac gccaccgtgg cgttcaccca
     6241 ccgcgacccg gtggccgtga tccagtcggc gatcacgatg ctcgcgtacg gagaccgcat
     6301 gcggcgcaag cacatcggcg ccgactggct gatcgaatac tggaccgacc gcatcgaacg
     6361 actgctgcgc cgctgcgtcg cagaccgcga cctgataccc gccgaccgca gcatcgacat
     6421 cggcttccat cacctcaacg gaggcgaaat gttctttgct gacaacgctc tacgagctca
     6481 atggcaccac gctcggcgaa agggcccgcg gcgctatgca gcgctacctc gacgacaatc
     6541 cccgcggaaa gcacggcagt gtccgatacg acctgcagca ccatttcggc cggtgcaccg
     6601 cgcgaactac gacgtggtcg acagctcgcc gatccgttac gtcatcctca cccagggcca
     6661 ctacgaccac gtcggcggca ccgatctgct cagcgacccg ggcaccgaca ttgtgatgca
     6721 agcgaactgg gagtcgtggc ggcgggacaa tgacctgctc gcgacgttcc gcgccgcgaa
     6781 cgca
//
LOCUS       DASWBF010000505         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1235778,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000505 DASWBF010000000
VERSION     DASWBF010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235778"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VF874_18540"
     CDS             complement(<1..1365)
                     /locus_tag="VF874_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9177979.1"
                     /translation="MTDTRNKRRGQKGAVGLQRHKRTPTAIGLALVTPIVGQEFLDRY
                     RLRDPLNRALRYGIKQAFSVAGASTRQFKRLQGLRSGPARLKSSDKNYFDLTPDDEQK
                     MIVDTVSEFAEEVLRPAAREADDAATYPAELIGKAAELGITAINIPEDFEGIAAHRST
                     VTNALVAEALAYGDMGLALPILAPGGVASALTNWGSADQQATYLKEFAGENVPQACVA
                     IAEPHPLFDPTALKTTAVRTPSGYRLSGVKSLVPAASNAELFIVGAQLNGKPALFIVE
                     SSSQGLTVTADPSMGIRAAALGQVNLDNVSVPLSARLGEEASSDADYSEAIALSRLGW
                     AALAVGTAHAVLDYVVPYVKERQAFGEPIAHRQSVAFMCANIAIELDGLRLITWRGAS
                     RAEQGLPFAREAALANRLGTDKGMQIGLDGVQLLGGHGYTKEHPVERWYRDLRVIGVA
                     EGVVV"
     gene            complement(1468..>2328)
                     /gene="amrS"
                     /locus_tag="VF874_18545"
     CDS             complement(1468..>2328)
                     /gene="amrS"
                     /locus_tag="VF874_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX9177980.1"
                     /translation="FLPGSAVLSFGTAGCNLACKFCQNWDISKSREIDTLSGRATPAD
                     LARVADELGCRSVAFTYNDPTIFWEYAADVADACHERGIRAVSVTAGYMCPAPRAEFY
                     RHIDAANVDLKAFTEDFYRKVCAAHLNDVLDTLVYLRHDTDVWLEITTLLIPERNDSD
                     DEIAAECAWIRDNLGMDVPVHFTAFHPDYKMTDTPRTPAATLTRARGIALAEGLRYVY
                     TGNVHDTAGGTTPCPGCGAAAVVRDWYAMRHYALTDDGRCRNCGAQLPGVYDGPAGRW
                     GAQRLPVQIA"
BASE COUNT          359 a          778 c          786 g          405 t
ORIGIN      
        1 aactacaacg ccctcggcga cgccaatcac gcggaggtcg cggtaccagc gttcgacggg
       61 atgttccttg gtgtagccgt ggccgcccag cagttgcacg ccgtcgagcc cgatctgcat
      121 gcccttgtcg gtgccgagcc gattggccag cgccgcctcc cgcgcgaacg gcaggccctg
      181 ctcggcgcgc gaggctccgc gccaggtgat cagccgcagc ccgtccagct cgatcgcgat
      241 gttcgcgcac atgaacgcaa ccgattggcg atgggcgatc ggctcaccga acgcctgccg
      301 ctctttgacg tacggcacca cgtagtcgag caccgcgtgc gcggtaccca cggccagcgc
      361 agcccagccc agccgcgaca gcgcgatggc ttcggaatag tcggcatcgc tggacgcctc
      421 ctcacccagc cgcgcgctca acggcaccga cacgttgtcg aggttgacct gtcccaacgc
      481 cgcggcccgg atccccatgc tcgggtcagc cgtgacggtc agcccttgcg acgacgattc
      541 cacgatgaaa agcgctggct tgccgtttaa ttgggctccg acaatgaaca actcggcgtt
      601 ggaagccgct ggcacaaggg atttcacgcc ggaaaggcgg tacccgctcg gcgtgcgcac
      661 ggcggtggtc ttcagcgcgg tcgggtcgaa caacgggtgc ggctccgcga tcgcgacgca
      721 cgcctgcggc acgttctccc cggcgaactc cttgagatag gtggcctgct ggtcggcgct
      781 gccccagttg gtcagcgcgg acgcgacgcc gccgggcgcg aggatcggca gcgccaatcc
      841 catgtcgccg tacgccaatg cctcggccac cagcgcgttg gtcacggtcg agcggtgcgc
      901 ggcgatgccc tcgaagtctt ccgggatgtt gatcgccgtg atgccgagct cggcggcctt
      961 cccgatcaac tccgccgggt acgtcgccgc gtcgtcggcc tcgcgggccg cgggccgcag
     1021 cacctcctcg gcgaactcgc tgaccgtgtc gacgatcatc ttctgctcgt cgtcgggggt
     1081 caggtcgaag tagttcttgt cgctggactt cagccgggcg gggccgctac gcagcccctg
     1141 taggcgcttg aactgccgcg tgctggcacc cgcgacggag aacgcctgtt tgatgccgta
     1201 gcgcagggcc cgattcagcg ggtcgcggag acggtatctg tccaggaact cctggccgac
     1261 gatgggtgta actaacgcca gtccgatggc ggtgggagtg cgcttgtgcc gttgcaggcc
     1321 taccgcgccc ttttggcccc ggcgcttgtt tcgggtgtcg gtcatgtcag cagcctcggt
     1381 gggtgggtgg cgatgccggt aaccgtatct aactccagag taaggtcgaa gcggttgtta
     1441 gctgattcac agtgagcgaa cacttgttca cgcaatctga accggcagcc tctgcgcgcc
     1501 ccaacgcccg gccggtccgt cgtacacacc gggcagctgg gctccgcagt tgcgacagcg
     1561 cccgtcgtcg gtgagggcgt agtggcgcat cgcataccag tcgcgcacga cggccgccgc
     1621 gccgcatccg gggcatggcg tcgtaccgcc cgcggtgtcg tggacgttgc cggtgtagac
     1681 gtagcgcagg ccttcggcca gcgcgattcc ccgcgcccgc gtcagcgtcg ccgccggcgt
     1741 gcgcggggta tcggtcatct tgtagtccgg gtggaacgcg gtgaagtgca ccggcacgtc
     1801 catgccgagg ttgtcgcgaa tccacgcgca ctccgcggcg atctcgtcgt cgctgtcatt
     1861 gcgttccgga atcagcagcg tcgtgatctc cagccacacg tcggtgtcgt gcctgaggta
     1921 gacgagcgtg tcgagcacgt cgttcagatg cgccgcacag accttgcggt agaagtcctc
     1981 ggtgaacgcc ttcaggtcga cgttcgcggc gtcgatgtgc cggtagaact ccgcgcgtgg
     2041 ggcggggcac atgtagccgg ccgtcaccga cacggcgcgg atgccgcgct catggcaggc
     2101 gtcggcgacg tcggcggcgt actcccagaa gattgtcggg tcgttgtagg tgaaagccac
     2161 gctgcggcaa cctaattcgt ctgcgacccg ggccaggtcg gcaggcgtcg cccgaccact
     2221 caaagtgtcg atctcgcggg acttcgagat gtcccagttc tggcagaact tgcacgcgag
     2281 gttgcatccc gcggtgccga aagacaggac cgccgagccc ggcaggaa
//
LOCUS       DASWBF010000506         3570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_567834,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000506 DASWBF010000000
VERSION     DASWBF010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567834"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1120)
                     /locus_tag="VF874_18550"
                     /pseudo
     CDS             complement(106..1120)
                     /locus_tag="VF874_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726904.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            1305..2684
                     /locus_tag="VF874_18555"
     CDS             1305..2684
                     /locus_tag="VF874_18555"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9177981.1"
                     /translation="MQETTVVVIGAGITGIGSAYYLRDSGIPYVVLEANSDLGGIWYT
                     QRWHGARCDSDFVKYSYSFKPYTSPRCLEDREQIHRYLRLVAEEFSILENIRFDTRVT
                     SASYDTETQSWVVQTNRGSIRAQFLINGNGYFSLPHVPKFSGAESFGGEIVHTFDLDA
                     ERCFPGKDVVLVGSGSTAICAAPELARVSGSMTMLQRSPSYIYEIDNRATPFMRLCQY
                     LYGRGFALPMKLLRGYLQARDDAIFVGFRTFPRIARWFFRRHWGGTVSREDLKEHFSP
                     SYSPWEQRIPVAIGFKEAVRRGAVRMKTGAIERFTPTSIVMRDGEELPCDVCILATGF
                     ELAFLKFDLIVDGEKVDMAGINFYKGIMMGGVPNYFQPVGVWHSAWTQRSEAVTRFAV
                     KILKYMASSGLREVRIERQDVQYTPAITPNYLMRDLPKLPRLYGSWELPSVDNLFRYR
                     FEPRKLNFS"
     gene            2702..3547
                     /locus_tag="VF874_18560"
     CDS             2702..3547
                     /locus_tag="VF874_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX9177982.1"
                     /translation="MLLLSRVTGHDVLGPDGRAAGRVADLTVRLGAPAGPHLVERIFV
                     RDGATELLIPWAEIESVRHTDIALASGDVSRFASTADTLDDDEILLVRDVLDTQIVDV
                     VGHRLARVADVVLARTAHNRLELVGVEVGFAGVLRRLGLHRLAARAGEDAVAWTDLHL
                     TSERGHAVQLATPRSAVHHLDARALAALVSRLDTESAAEVLAVKGPDVAADVVRAAHP
                     VVGERVLRAMPDAHVAQIVAAMPAEHATHWRRRLAQVPGRRFLRSRVWPRRRHLPRRR
                     TGETR"
BASE COUNT          586 a         1262 c         1125 g          597 t
ORIGIN      
        1 cccgccaggc cgtcgcgacc taaccaccat cgggtgatgc cccgcatcac caacgcgatc
       61 gccaacggcg acccaaagat gacattgccc ttcgtagcat cgccgcaaca agtcctcatc
      121 ctcggcgtca gaagtctgag cccgtatccg agcgcgcgct gcccgcgggt caagaccctc
      181 gacgccggtg gtcgcgcgca acagccgcga gacggcgtgg aagggaatct gactggtgtg
      241 cgactcgcag aacgcggtga acacctcgac accacgggcg gctgccatcg ccgaaacttc
      301 atgtacgagg cggcttttgc caatgccagg tgagccgacc acgcccacaa ctgcgccgtg
      361 gccgtcgatt gcacggtcca gcaagccctc gacggcggac atctcccagc gccgaccgac
      421 cagattcgac tcaccacgcc tgcgatcgcg gtgcggttcg accatgccca ataaccgacg
      481 ggcccgcacc ggttcagctg cgcctttgac cgaaacgaac tcgggctcgg cctgagccgc
      541 tgcgccgtcg accagccgtg cggtcgacgc gctgagcatg atgccacccg gtggagcgac
      601 cgactccatc cgctgtgcca ttcccacctg ttcaccgacg gccgtgtaac cgaaagggcc
      661 cgaaccgatt tcgccggcga tgacctgacc ggaattcaga ccgatgcgta gtcgtagtgt
      721 gatgtggtca cggtggttca acctcagctg ctaggcgatg agtttcggcc tgaatcccca
      781 gtgcggccat acacgcgcga gaagcatggt cctccaacgc gactggcgcg ccgaacaccg
      841 ccatgatgcc gtcgccggtg aacttgtcca ctgtgccgcc gtagcgcttc atcaccgcgg
      901 ccgaacgctc gaccaactcg gtcatgattt cgcgcaaccg ctccgcgccc accgcggccg
      961 cgatgtccat cgagtgcacc acgtcggcga acaagacggt cacctgcttg tattcggcgt
     1021 tctcggtggc aggcgcaatg accgaaccgc aggcgtcaca aaaccgcgcg ccctcccgcg
     1081 gttcggtgcc gcatgtacgg cacgcggtcg ctgcggtcat ctcctccacc gcgcccggac
     1141 ccaagattcc agggttcatc cacgataggc cgctgctcga tgcgattccg ccgcaaccgt
     1201 gtttctgcgg cttgtcgtgt ggcaaactgc atgttggtgg ggagtttgct gtgtcggcgg
     1261 ctacgtaaac cccccgcggg gaggttcgca tcaacgccac ggcgatgcag gagacgacgg
     1321 tcgtcgtcat cggcgcggga atcacgggaa tcggatcggc gtactacttg cgcgacagtg
     1381 ggatccccta cgtcgtgctc gaggccaaca gcgatctcgg cggaatctgg tacacgcagc
     1441 gctggcacgg cgcgcgctgc gactcggact tcgtcaagta ctcctacagc ttcaagccct
     1501 acacatcgcc gcgctgcctg gaggaccgcg agcaaatcca ccgctacctg cgcttggtcg
     1561 cagaggagtt ttccatcctg gagaacatcc gcttcgacac gcgtgtgacg tcggcgtcgt
     1621 acgacaccga gacacagtcc tgggtggtgc agaccaaccg cggcagcatc cgcgcgcaat
     1681 tcctgatcaa cggcaacggc tacttctcgc tgccgcacgt gccgaagttc tcgggcgcgg
     1741 agagcttcgg cggcgagatc gtccacacat tcgatctcga cgcggagcgc tgtttccccg
     1801 gcaaggacgt ggtgctggtg ggcagcggct cgaccgctat ctgcgccgcg ccggagctcg
     1861 cgcgtgtctc cggttccatg accatgctgc agcgctcgcc gtcctacatc tacgagatcg
     1921 acaaccgcgc caccccgttc atgcggctgt gccagtacct ctacggccgg ggcttcgccc
     1981 ttcccatgaa gctgctgcgc ggctacctcc aggcgcgcga cgacgccatc ttcgtcggct
     2041 tccgcacgtt cccgcggatc gcgcgctggt tcttccgccg gcactgggga ggcacggtga
     2101 gccgcgagga cctcaaagag cacttctcgc cgtcctactc gccgtgggag cagcgcatcc
     2161 ctgttgccat cggcttcaag gaggcggtgc ggcgcggcgc ggtgcgcatg aagaccggcg
     2221 cgatcgagcg cttcacgccc acatctatcg tcatgcgcga cggcgaagag ctgccatgcg
     2281 atgtctgcat tttggccacc ggcttcgagc tggccttcct caagttcgac ctgatcgtgg
     2341 acggcgagaa ggtcgacatg gccgggatca acttctacaa gggcatcatg atgggcggcg
     2401 tgcccaacta cttccagccg gtcggtgtgt ggcactcggc ttggacgcag cgctcggagg
     2461 cggtcaccag gttcgccgtc aagatcctga agtacatggc gtcgagcggg ctgcgcgagg
     2521 tgcgcatcga gcgccaggat gtccagtaca cgcccgccat cacacccaac tacctcatgc
     2581 gcgacctgcc caagttgccg cggctctacg gcagctggga gctgccgtcg gtggacaacc
     2641 tgtttcgcta ccgcttcgag ccgcgcaagc tgaatttctc ctagccgggc atgctcctag
     2701 agtgctcctg ctgagccggg tgaccggtca cgacgtgctc gggcccgacg gccgggcggc
     2761 cggtcgcgtc gccgatctca ccgtgcggct tggcgccccg gccgggccgc atctggtcga
     2821 gcggatcttc gtccgcgacg gcgccaccga gctgttgatc ccgtgggccg agatcgaaag
     2881 cgtccggcac accgacatcg cgctggcctc cggcgacgtc agcagattcg cgagcaccgc
     2941 cgacaccctc gacgacgacg agattttgct ggtacgcgac gtgctcgaca cccagatcgt
     3001 tgacgtcgtc ggccaccgac tcgcgcgtgt cgccgatgtg gtgctggccc gcaccgccca
     3061 caaccggctg gaactcgtcg gcgtcgaggt cgggtttgcc ggagtgctgc ggcggctcgg
     3121 cctgcaccgg ctcgcggcgc gcgccggcga ggacgcagtc gcctggaccg acctgcacct
     3181 gacctccgag cgcgggcatg ccgtgcaact cgcgacccca cgctcggcgg tacaccacct
     3241 ggacgcacgc gcgctcgccg cgctggtcag ccggctcgac accgagtcgg ccgccgaggt
     3301 gctcgccgtc aagggccccg atgtcgccgc ggatgtcgtg cgcgccgctc atccggtcgt
     3361 cggcgagcgt gtgctgcgcg ccatgcccga cgcccacgtc gcgcagatcg tggccgcgat
     3421 gccggccgag cacgcgaccc attggcgccg ccgcctcgcg caagttcccg gacgacggtt
     3481 cctgcggtcg cgcgtctggc ctcgtcgccg ccacctcccg cggcgacgca ccggagaaac
     3541 gcgttgagac gacggctggg tatcggcgca
//
LOCUS       DASWBF010000507         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_309201,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000507 DASWBF010000000
VERSION     DASWBF010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309201"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF874_18565"
     CDS             complement(<1..219)
                     /locus_tag="VF874_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9177983.1"
                     /translation="MLTRTAALAFGERHYSHAEIEALTDGLAATLRGRGVRSGQRVAL
                     MSSNRPEFVVAVRAIWRLGATVVLLSPAW"
     gene            292..1293
                     /locus_tag="VF874_18570"
     CDS             292..1293
                     /locus_tag="VF874_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX9177984.1"
                     /translation="MAITHVFHRATVARIIKETADASTFVLTPDEPFSYRAGQYCTFR
                     VTVDGEDLYRSYSMSSAPETDAELATTVKRVPAGKVSNWMLDNLVEGDEVVLTRPAGT
                     FCLDSSSAPLLGFVGGSGVTPILSLAKSALATTKRQVRLLCADPDRPSVIFDAAMAEL
                     VDRYPDRLEVVRHIDAERGLLDAATVRMFVGPDVDADCYVCGPEGFMTVVKSALPGPG
                     RVFVENFDIEPKVHTTPPADAKPATGGTVKIHLDRKKVSVPRVAGETLLESARRGGLA
                     PPFSCEAGNCGTCIAKLTDGHATMRANDVLEDDEIADGYILTCQGIPDSESITVVYE"
     gene            complement(1284..2336)
                     /locus_tag="VF874_18575"
     CDS             complement(1284..2336)
                     /locus_tag="VF874_18575"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9177985.1"
                     /translation="MTDGPLAGIRILEVGTMLAGPYATMMLADLGAEVIKIEPPGGEI
                     SRQVSDSYFASLNRGKRSITLELNSEAGQRQLGELVKDAHALLVNLKPSAIRRLGLTY
                     DALKRHNERIVCVALTGFGLDGGDEPAFDYVVQAATGIAALTGDPDGPPTLPGYSSAD
                     NSTGLTAALGLLAQIVSGRGGQVDVCLQDVMLSQLNYRASAYLNDGVEPRRYPFGAHS
                     YYVPAQLFPTADGYLALFVTHDAFWKLFAAEANIAGFETMAERVSRRDDVLAVVADAL
                     AKDTAKGWEARLRPLGIPAAAVRSLPEALEADPGIVVTAGDFRLVGSPIRVSGYTPDY
                     GPPPKLGEHNDVLPHS"
     gene            complement(2333..>2548)
                     /locus_tag="VF874_18580"
     CDS             complement(2333..>2548)
                     /locus_tag="VF874_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9177986.1"
                     /translation="RLLDQGEAKDVGDICAAGDEEAILARFRSFADAGVTDLSVRLLP
                     IGDNRDELIASKRRTREVIAELAALVR"
BASE COUNT          444 a          870 c          822 g          412 t
ORIGIN      
        1 ccacgccggg ctgagcagaa ccaccgtggc gcccaaccgc cagatcgcac gcacggccac
       61 gacgaactcg ggccggttcg acgacatcag tgctacgcgc tggccgcttc gcactccccg
      121 cccccgaagg gtggcggcca acccgtcagt caacgcctcg atttcggcgt ggctgtagtg
      181 cctctccccg aacgcaagcg cagcagtgcg agtcaacatc gagaggatcc taccgtgatt
      241 gagaacctga ttctcttatt gatagaatct tcgtaccaga ggggaggcgt tatggcgatc
      301 acccatgtgt tccaccgggc caccgtggct cggatcatca aggagactgc cgacgcgagc
      361 acctttgtgc tgacacctga cgagccgttt tcgtatcggg ccgggcagta ctgcaccttc
      421 cgggtgacgg tggacggcga agacctctat cgctcctact cgatgtccag cgcccccgag
      481 accgacgccg aactggccac caccgtcaaa cgcgtacccg ccggcaaggt gtccaactgg
      541 atgctcgaca acctggtcga aggcgacgag gtggtcttga ccagacccgc cggaacgttc
      601 tgcctggact cgagttccgc gccactgctc gggttcgtcg gcggcagcgg tgtcaccccg
      661 atcctgtcgc tggccaagag cgctctcgcc accacgaaac gtcaggtgcg gctgctgtgc
      721 gccgaccccg accggccatc ggtgatcttc gacgcggcga tggccgaact cgtcgaccgc
      781 tacccggacc ggctcgaggt ggtgcgccac atcgacgccg aacgcgggtt gctcgacgcg
      841 gccaccgtcc ggatgttcgt cggtcccgac gtcgacgccg actgctacgt gtgcggcccg
      901 gagggcttca tgacggtcgt gaagtccgcg ctgccgggac cgggccgggt attcgtcgag
      961 aacttcgaca tcgaaccgaa ggtccatacg actcccccgg ccgacgcgaa gcccgcgacc
     1021 ggcgggaccg tgaaaatcca tctggaccgc aagaaggtat cggttccgcg ggtggcgggt
     1081 gaaacgctgc tggaaagcgc ccggcggggt ggactggcgc cgccgttctc ctgcgaggcg
     1141 ggcaactgcg gcacctgcat cgcgaagctg accgacgggc acgccacgat gcgagccaac
     1201 gatgtcctcg aagacgacga gatcgccgac ggctacatcc tcacctgcca aggcattccc
     1261 gacagcgagt cgatcaccgt cgtctacgag tgaggcagaa cgtcgttgtg ctcgcccagc
     1321 tttggcggtg ggccatagtc cggggtgtag ccgctcaccc gaatggggct gcccacgagc
     1381 ctgaaatcac ccgcggtgac gacgatccct ggatcggctt ccagcgcctc cggcagtgag
     1441 cggaccgccg cggcagggat cccgagcgga cgcagccgcg cttcccagcc tttcgcggtg
     1501 tctttcgcca gagcgtccgc gacgacggcg agcacgtcgt cacgacgtga cacccgctcg
     1561 gccatcgtct cgaaccccgc gatgttcgcc tccgccgcaa acaatttcca gaaggcgtca
     1621 tgggtgacga acaacgcgag atatccgtcg gcagtcggaa aaagctgcgc tggaacgtaa
     1681 tacgagtgag cgccaaacgg gtatcggcgg ggttcgacgc cgtcgttgag gtaggccgac
     1741 gcgcggtagt tgagctggga cagcatcacg tcttgcaggc acacgtccac ctggccgccg
     1801 cgacccgaga cgatctgcgc cagcagaccc agtgcggcgg tcaagcccgt ggagttgtcg
     1861 gccgaggagt accccggcag cgtcggcggc ccgtccggat cgccggtgag cgcggcgatg
     1921 ccggtcgcgg cttgcacgac atagtcgaac gcgggctcgt cgccgccgtc caacccgaac
     1981 ccggtcagcg cgacgcagac gatccgttcg ttgtgtcgct tcaacgcatc gtaggtgagc
     2041 ccgagccggc ggatcgccga cggcttgagg ttgaccagca acgcgtgtgc gtccttgacc
     2101 agctcaccga gttgtcgttg tcccgcttcg gaattcagct cgagagtgat gctgcgcttg
     2161 ccccggttca ggctggcgaa gtaactgtcg ctcacctgcc gggagatctc gccgcccggc
     2221 ggctcgatct tgatgacctc cgcgccgaga tcggccagca tcatcgtcgc gtacgggccg
     2281 gccagcatgg tgccgacctc gaggatgcgg atgcccgcca gcggaccgtc cgtcatcgaa
     2341 ccagcgcggc gagctccgcg atcacctcgc gggtacgccg cttggacgcg atcagctcgt
     2401 cgcggttgtc accgatcggc agcaggcgca ccgacaggtc ggtgactccg gcatcggcga
     2461 acgagcggaa ccgcgccagg atcgcctcct cgtcgcctgc cgcgcagatg tcgccgacgt
     2521 ccttggcttc accctggtca agcagccg
//
LOCUS       DASWBF010000508        25298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1167358,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000508 DASWBF010000000
VERSION     DASWBF010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 25298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25298
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167358"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2632
                     /locus_tag="VF874_18585"
     CDS             <1..2632
                     /locus_tag="VF874_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9177987.1"
                     /translation="AGDCGACTVWLDGNPVHSCITPAFRADGREVTTVEGLGSPDQMS
                     PLQRQLRDAPGFQCGFCTPGMIMTAASFSDEQKMDLPRSLKGNLCRCTGYRAIEDAIR
                     GVAGIEDAEAGKAVGVSVGAPAGTEVVTGTAEYTMDTHIDGMLHLKVLHSPHAHARIR
                     SIDKTAALAVSGVHRVYTWEDVPRKIYSTAIHTDHLVDPDDTYILDDVVRFVGQRVVA
                     VVADTIAAAEEGCRKVVVDYEVLLAVFDPELAMADGAPQLHGSTDPFVHDPIRNILVE
                     LHGEIGDVETGFAEADVIHEGTYFSPRVQHVHLETHGSIAWMADGRLNVRTSSQSPSI
                     AKQKLEYLFDLRPDQLRVFCKRVGGGFGGKQELIAEDLVALAAQDIGRPVCWEYTREE
                     EFTTASPRHPMKITVKLGARADGTLTALQFRNVSNTGAYGNHGGETLFAGAAAVALYR
                     CPNKKFDGYSVYTNTVPSGALRGYGMTQPAFAVESAIHELAVALDMDPLELRRRNIVR
                     PGDSLLAIHDEPDDVEFTEDGLAQCIDLVDDALRRNAAHARSNWDTAGQTLGDDWLVG
                     AGTASSLHETAPPTEHISEAWATLGDDDIYEIAVGTVEFGEGTSTVHVQLAATVLCTT
                     PSRVRLVQSDTDRNGFDTGAFASAGMFVAGNAVLRAATALRDRILRFAASHSNSHVSA
                     CVMDDDAVRCGHTRVPLSELVAAARQRGIRFTESRKAYGSPRSVTSNTHGFRVAVHRV
                     TGEIRVLYSVQATDAGVVINPVQVRGQVEGGVAQGIGFVLTENHHVDGNGVMTNPSLR
                     NYRIPTFADAPRTEVILVDSTDSVGPMRAKGMAECCTNPVAPALANAVHDATGVRFRE
                     LPLTPERIYDRLPSRELAKR"
     gene            2629..3600
                     /locus_tag="VF874_18590"
     CDS             2629..3600
                     /locus_tag="VF874_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019748694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX9177988.1"
                     /translation="MSAGNVTDNAATVIIGQRLKPGCEEAFLAWQHDLNSTASRYPGF
                     VAAEVTPPTAVQPDWTVVYRFDTIANLRAWLNSATRQDRLPEGRPFFEAPPTQQVIRG
                     SAKAPDELVTVVVTHRVAPEHLQEFLQWQDRLREAESRFPGFRGSELFRPIPGVQDDW
                     TAMYRYNTAADLDAWLKSPERQQLLAEGKNFEDFELRTVDNSFGSWFAFDDGAADAKP
                     PSDFKTSIAVWVGLYPTVVLLTLATSPLKMPLWLGMLVGNLLSSFAMTFVTMPFYVNP
                     LLKKWLRLPDGVPKTRVNVRGVVLIAAVMAAWTLAFYLITRVFWHLP"
     gene            complement(3604..6780)
                     /locus_tag="VF874_18595"
     CDS             complement(3604..6780)
                     /locus_tag="VF874_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011597278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX9177989.1"
                     /translation="MKLRRARSIMAIGLVVALTLMLGSCSSDKPESPPGPQAGVFAGP
                     DGKPAPYREPVKLSSKDGVLEVRLSAHQGTVALDTVKQPVTNFLVFGYELIKGTSSDG
                     STKGDNLYPAPTLRVDPGQRLIVHFDNDLQGLSIADFNNPAMTPAGGDVPIYPPALES
                     APINLHTHGLHVSPSGNADNVLLNIPPGMGNTYDYAVPTDMPNGLYWYHSHRHTLTAQ
                     QTYMGLAGLLEIGRPDGNLPIVTQNNIPIRVMALQYNFVFDRKGGGHQLNNPYWEQWV
                     STLKPPEGSQLADGTYSPSLAPVNFSETTKGAEYFTNWYQGPLSIHNHRGQNQFIPQN
                     LQSFTSPSRNVPANPSLPDNQRDVQFTVNGQFQPELKLKPGQTEIWVLANISDIAYMP
                     VRFTETATGTHPKFSIVGQDGNPYTQVQRPVDGDGTYLEIPPASRYAIAVTMPKSGDL
                     VLDMPPKDGVKGISEPGVLYTNNGTRNSPAVLGTVTIDPKYISARDGFFTFPTQTLLH
                     VTPDSGEGQTTAFEPGQNLDAFTSFVDTSVMTPAVRRELMISGGFGNKNASNNDPKAF
                     TYQFDDNIFPNIPLIQPRLNSVEEWAITNYNNDGHPIHIHVNDFQVQEVVSPLIGTKT
                     GVQPFGVDNANVPPPTIDANDQALAPASLTLRSEFLEYAGTFVIHCHRLNHEDNGLMG
                     TVNVIPEVSTYAVAVPGSNGKPATVQVHDGNGDTVIATVYPFPAFEGTPSVAMADVNG
                     DMILDLIVGTGPGTDSEVVVYSGSDTPDGPFRTELTRFAPFDAGFRGGVNVAGANIDG
                     NALAANIIVGSGPGMESEVKVFSSKLPTEKGIAPDVFSSFKPYPGSQAGVTIATGLVE
                     METGRESIVTAPGPGEPAKVRTFRYDLYTPTARALANGAPQQQIGPGEPAMSAELLAY
                     DESYTGGVSLSTGWVAGQEGGAKSIITGQLAGDGTVRVWSAGSRLDGQPAMYTHSPDQ
                     HSGHVMFRQTASFNPFTGGAPGVTVATTSTTSGADLLVSGAGPQGAEVVKFGLARADP
                     QANTLTPNVLTRLPASAGVNGPVPLGGR"
     gene            complement(6814..7143)
                     /gene="mnhG"
                     /locus_tag="VF874_18600"
     CDS             complement(6814..7143)
                     /gene="mnhG"
                     /locus_tag="VF874_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEX9177990.1"
                     /translation="MTALDVVTAVLVLSGSALALTASIGVVRFPDTLSRMHAATKPQV
                     LGLLLVLVGAAVRLRGNVDVGMIILTAMFTLITAPVVANRVGQLAYREQNIRDDLLTT
                     DELREDS"
     gene            complement(7130..7414)
                     /locus_tag="VF874_18605"
     CDS             complement(7130..7414)
                     /locus_tag="VF874_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEX9177991.1"
                     /translation="MTTVWMIAAVMLAAAAALTTFRILAGPSTLDRLVGVDTLVAVAM
                     CGLGTWAAFSLDSTVVYGITALSLISFVGSVSVARFRVPDVESKDGDDRA"
     gene            complement(7411..7944)
                     /locus_tag="VF874_18610"
     CDS             complement(7411..7944)
                     /locus_tag="VF874_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX9177992.1"
                     /translation="MSRRWALRVWSVCWLVAVWILLWGTISAANILSGLAVALLITFL
                     LSLPVVPIEGRLHPLSLAKLNVVIGRNLVVSSLQVAWLAIKPGPPPMSAVLRVRLAVK
                     SDLVLVLAVHAINITPGTMVLEIDRDRRLLYVHVLDVGSPRAVNRFYNEVAQLERLLI
                     AAFERDKDWRPVAEEQA"
     gene            complement(7941..9539)
                     /locus_tag="VF874_18615"
     CDS             complement(7941..9539)
                     /locus_tag="VF874_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HEX9177993.1"
                     /translation="MSIASALTPLPVLIPALGAALTMFVGRHPRLQRTISLAALAAVV
                     AVCVTLIYLTDKCGTIAVQVGGWGPTVPGLGPLGITLVVDRLSALMMVVSSIVLLAVV
                     LYAIGQGIRDGDERQPVSIFLPTYLVLSAGVCGAFLAGDLFNLFVGFEVLLTASFVLL
                     TIGASADRVRAGIAYVMVSMVASLIFLFALALIYAATGTLNMAELAVRLDDVAPGTRS
                     ALFAVLLVAFGIKAAVFPLSTWLPDSYPTAPAPVTAVFAGLLTKVGVYAIIRAHSLLF
                     PSGGLDSVLLVAALLTMLVGVLGAIAQNDIKRLLSFTLVSHIGFMVFGIALANRPGMT
                     GAIYYVAHHIIVQTTLFLVAGLIERQAGASTLRRLGGLATASPLLALLFLLPALNLGG
                     IPPFSGFLGKVALLQAGAQEGSALAWVLVGGAVTTSLLTLYAVTRVYSKAFWRPRADA
                     PEGALSNAGPTVLLDDPEADVTFLDRDDVGRMPFGMLAPTVALIGVGLALTVLAGPFF
                     SYADRAAAEMLDRDRYITAVVGTR"
     gene            complement(9536..10009)
                     /locus_tag="VF874_18620"
     CDS             complement(9536..10009)
                     /locus_tag="VF874_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit C"
                     /protein_id="HEX9177994.1"
                     /translation="MTTPLVPLVLIGVLTSSGVYLLLERNLTRMLLGLLLIGNAVNLL
                     ILTVGGPSGNPPIFGRTSAGRSSTADPLVQGMILTAIVISMGVAAFVLALAYRSYRLT
                     TAEEVPDDPEDTRVSQQTEDEVEEDVVHPEPELDVDEPAELDAVPAHSRKAQELT"
     gene            complement(10006..12834)
                     /locus_tag="VF874_18625"
     CDS             complement(10006..12834)
                     /locus_tag="VF874_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit A"
                     /protein_id="HEX9177995.1"
                     /translation="MLAILLAHAVAAVAAPMLVYRWGRMAFYPLAVVPAASLIWVALN
                     WPQAGHVNTVHQQWVPELSLNVDLRFDALSAIMAVLVLGIGALVLVYCADYFHHHDGH
                     TEQRLPSFAAQLVAFAGAMFGLVVSDNMLLLYVFWELTTVLSFLLVAHYAERATSRRA
                     AMQALLVTTAGGLAMLVGIIVLGHTSGTYLLSELVADPPSGALASVGLALLLVGAVSK
                     SALVPLHFWLPGAMAAPTPVSAYLHAAAMVKAGVYLVARLTPGFADAPPWRPTVVILG
                     VGTLLLAGWRAVREYDLKLILAFGTVSQLGFITLLVGAGGGNLMLAGLALLFAHALFK
                     AALFMVVGIIDHSTGTRDIRRLAWLGHECRPLFVIAAFATASMAAVPPFLGFVAKEAD
                     FETLAHSASLGAAAPFVLAAVVLASVFTTIYSLRFLWGAFARKGRTEPSSLVAHMHRP
                     SSSFLVAPAILAAASLALGIVPDWLDDVLDNYADTMPGGHSYQLELWHGVGLALALSA
                     VVLGVGAGAFVLRNRLRRTRIGRLPLGNADRGYDAVMRAADMVSLKVTAVTQRGSIPA
                     TQAVILSTLVLLPVGALLVGARDRPQFRLWDSALQLLVGLLIVAAALGTTVMRNRLAG
                     VLLVGITGYGCGVIFALHGAPDLALTQFLVETLTLVVFVLVLRALPAEAQAANIKRRR
                     GPRVVLALAVGATVTTLAAFAMAARTGAPIASLLPDAAYHRGHGANTVNVLLVDIRAW
                     DTLGEIMVLLVAATGVASMVFRHRRFGAAPRVSDAPHPAPVSSDVTWLRGSELRDPRH
                     RSLVLEVATRVIFPLIMVLSVYFFFTGHNTPGGGFAGGLTAGLALVLRYLAGGRYELG
                     ETLPIDVGKVLGVGLALSAGTTLASLAFGAPVLSSALIEADVPVLGHVKFVTALFFDL
                     GVYLIVVGLVLDVLRSLGAHLDDELALR"
     gene            complement(12839..13243)
                     /locus_tag="VF874_18630"
     CDS             complement(12839..13243)
                     /locus_tag="VF874_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9177996.1"
                     /translation="MNAQHERVLLDTSVVIDFPGEAVGSLAATAAVSTITLAELAYGL
                     HTEDPLLNIVREQRYHWITSTFAPIPFDTSAARVYGALCASVRAIGRDPRPRRFDLLI
                     AAVAVALRIPLITRKESDFVGIHHALNIVAVQ"
     gene            complement(13240..13530)
                     /locus_tag="VF874_18635"
     CDS             complement(13240..13530)
                     /locus_tag="VF874_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9177997.1"
                     /translation="MATTISQRELRNDSGTIMRRVEHGERFTVTRNGIPVAELVPHDP
                     IAFVVRPQFVPVTEIAAGLSGLPSWDVERFKQERKVLDESVDDRDADRWSAP"
     gene            13652..15115
                     /locus_tag="VF874_18640"
     CDS             13652..15115
                     /locus_tag="VF874_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9177998.1"
                     /translation="MNASDVIVVGAGPTGLMLASELAMRGIDVRLLEERRDIPNITRA
                     FGVHARTLELLDARGLADEIVQRGLPVTEVAPAPGAVLNLKELPSRYPMVLMAPQSLT
                     ERVLTARAGQLGIEILYGAQVVGLEQHTGGVSVRLADGTTERAKYVVGCDGAHSTVRR
                     LLGIGFAGKQYQTHILLADVQLAQPPTDGLSAATSPDGVVLLVPFGDGWFRAIAWDRT
                     REQAPLSEPVTLSEIRDAFLRIGKTDFGMGEMRWSSRFLSERRQAERYRVGRGFIAGD
                     AAHVHSPLGAQGMNTGIQDAMNLGWKLAAAIGGTAPEWLLDSYQTERHAVGANVLKLT
                     DTFNQLVLGRSLLRRAFRRVAITVLLNVGVTKRMMAGRLSGIGIHYPRPRGAHPLTGQ
                     RMPDVDCSGTRLYELMRDGRLVLLTRCSVDVGRNDVTVAVHADPALPEAVLVRPDGYV
                     AWAGREAESAAALNHWTPASPRHSNLRGDTPAKAADA"
     gene            complement(15138..17288)
                     /locus_tag="VF874_18645"
     CDS             complement(15138..17288)
                     /locus_tag="VF874_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9177999.1"
                     /translation="MASLTLTARLNTAAVDSRRGVVRLHAEAIAALGIREWDALSLTG
                     SRTTAAVAGLAGPDVPAGTILLDDVTLSNAGLREDSPVILAPVTVYGAKSVTLSGSKL
                     ARQSASPATLRMALLGKVMTVGDTVSLLPRDLGPGTSTSEASAALASSVGITWTSELL
                     TVTGTDPDGPVSVQPNSSVTWGEGVAVTTAHAAAAYEPMPTISVDDLKGAEAQAARLT
                     EWLRLALDEPELLQKLGAAANLGVLVTGPAGVGKATLVRAVCAGRRLVELDGPDVGAL
                     AAQERLKSVADAVAKVRDGGGVLLITDIDALLPSPAEPVATLILAELRTAVGTPGVAF
                     IATSQAPDALDPRLRSPKLCDRELGLSLPDAATRKALLEVLLRDVPAENLNLSEIADK
                     TPGFVRADLAALVREAALRAAARASEDDTPPALTQDDFTGALTVIRPLSRSATEEVAV
                     GDVTLEDVGDMAETKQALTEAVLWPLQHPDTFSRLGIEPPHGVLLYGPPGCGKTFVVR
                     ALASSGRLSVHAVKGAELMDKWVGASEKAVRELFRRARDSAPSLVFLDEIDALAPRRG
                     QSFDSGVTDRVVAALLTELDGIEPLRDVVVLGATNRPDLIDPALLRPGRLERLVFVAP
                     PDADARREILRTAGKSVPLSAGVDLDALAADLEGYSGADCVALLREAALTAMRRSIDA
                     ADVTAADVAAAREKVRPSLDPVQVESLRAFAAGR"
     gene            complement(17295..18170)
                     /locus_tag="VF874_18650"
     CDS             complement(17295..18170)
                     /locus_tag="VF874_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEX9178000.1"
                     /translation="MTTATNQAHITLRFLPSAMTVAAISLGLTAVKYAQDRSLSIESR
                     ATWAMALLVLAAVLDALDGRVARVLNATSRMGEEIDSLADAVNFGVVPAFIVYAILLQ
                     DAPNPNAGWLVVVLYAACIVLRLARFNALLDVDQPAYAKNYFVGMPAPAGAVGAIGPI
                     AAKLQFGNGWWTWDWVVMLWLVGVSLLVVSRIPMRKVHTFSMPPNMVAPVLALFALGV
                     ASAFKFPYLTILVIIVAYVIHIPFAVRSKRWLANHPEAWDPKAKRRRALRRQIRRAQP
                     HGRSVARLGLRRPQR"
     gene            complement(18167..18880)
                     /locus_tag="VF874_18655"
     CDS             complement(18167..18880)
                     /locus_tag="VF874_18655"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX9178001.1"
                     /translation="MAKRPHLPADDSGSEASRLIELIRSTVPPMHRAGIPFVSAGLAV
                     AAIGRKNRWLRTAGLSAAGACAAFFRHPARVPPDRAGVVVAPADGQITIIDTAVPPAE
                     LGLPDQPMQRVSIFLSLFDVHVQRVPISGEVVAVQHRPGTFLSADKPQSSTENERNSM
                     WIRTPDGHDVVVVQVAGLLARRIRCDAKVGDKLNIGDTYGLIRFGSRLDTYLPDGSTV
                     KVIPGQRAVGAETILAELP"
     gene            complement(18887..20107)
                     /gene="glp"
                     /locus_tag="VF874_18660"
     CDS             complement(18887..20107)
                     /gene="glp"
                     /locus_tag="VF874_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX9178002.1"
                     /translation="MRSVEAHTRVVADLIRSRPAVTVAIADAAGLSLADDVVAPLSLP
                     GFDNSAMDGYAVMADDVAAATDENPVVLPVAEDIPAGRTDTLTLKPGTAHRIMTGAPV
                     PAGASAVVPVEVTDGGVETVEVRKAPKPGQHIRRAGEDVTAGVTVLRSGQVVTPAVLG
                     LAAALGLTELAVTPRQRVLVVSTGEELVAAGQPLRPGQIYESNAVMLAAAVQDAGGEV
                     VAAPMTGDDVDEFRRVLATYANDADLIVTTGGVSAGAYEVVKDSLSRDGIEFVKVAMQ
                     PGMPQGAGRLGAAGPATSSPSGTSVVTLPGNPVSALVSFEVFVRPALRAAMGLPSPHR
                     PLRKAVLTDGLTSPRGKRQFRRGVLDDGNVTAYGPPASHHLRWLASANCLLEIGEDVT
                     ELTEGSRVDVWDLR"
     gene            complement(20107..21024)
                     /locus_tag="VF874_18665"
     CDS             complement(20107..21024)
                     /locus_tag="VF874_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9178003.1"
                     /translation="MSSKWTAADIPDQSGRVAIITGANTGLGLETAKALADKGAHVVL
                     AVRNLDKGNQAVSQITAKSPNAVVALQQLDLTSLQAIREAAEEIKAAHQRIDLLINNA
                     GVMYTDKGTTKDGFELQFGTNHLGHFALTGLLLDRLLPIDGSRVVTVSSVGHRIMAKI
                     HFDDLQWERNYNRVGAYGQSKLANLLFTYELQRRLKLKGAPTVALAAHPGGSNTELTR
                     YIPGLIKQPVEFVWGLMAQSAEMGALPTLRAATDPAAQGGQYYGPDGFGEQRGYPKVV
                     ESSKRSHDEDVQRRLWTVSEELTGVTYPI"
     gene            21141..21575
                     /locus_tag="VF874_18670"
     CDS             21141..21575
                     /locus_tag="VF874_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX9178004.1"
                     /translation="MWALSGLLGWIVPVVAQVVWYVLQPSHGWLHLLVAAATAVLVLV
                     SVFVVPVWRYRVHRWEISSQAVFTRTGWLVQERRIAPISRVQTVDTHRGLLDRLFGLA
                     NVKVTTASSAGAVEIVALDTSVADRVVAQLTDIAALGGEDAT"
     gene            21602..22990
                     /locus_tag="VF874_18675"
     CDS             21602..22990
                     /locus_tag="VF874_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX9178005.1"
                     /translation="MLLVHPVHEVLRQLPVLVGAIFLGSSTGNSAWPLAALSAVVGFG
                     LLRWFFTTYRISDEYVELRTGVLRRRSISVPRNRIRSIQTDSRLLHRLLGLTVLRVGT
                     GQQSRSEGAVALDAVEAAQAPRLRAMLLAESLDVAEQPPGRVLARWQPSWLRYAPLSF
                     SGVGLIAAVIALAYQVGGVTLSERVAKAGAEAAHRTGIVVAVSVGAAGVLLVSVLLAV
                     LWSWLTYGGLLLRRDAGVLHLRHGVLRVREHTFDMRRLRGGTFREPLLVRFLRGGRLD
                     AVMTGVHGEGESSVLLPPCPAATAKAVLTDLIDDASAVSGPLRGHGPAAARRRWTRAL
                     AVPLLAGIAIVLAPLPVWVWAVWVAVTAACVLLAVDRVRSLGHRVADGWLVARTGSLE
                     RRRDCIACAGIIGWTVRQTLLQRRAGVATLIAATAAGRKHYRVIDVPAGLAWSVAAEA
                     SPWVANSIWASR"
     gene            23020..23829
                     /locus_tag="VF874_18680"
     CDS             23020..23829
                     /locus_tag="VF874_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX9178006.1"
                     /translation="MAVGGVLFDIDGVLVTSWKPIEGAAETVRILGEHQIACSYLTNT
                     TTRTRAQIAEALADAGITVRPDEVITAAALTAEYVRQNYPHARCFLVNSGDITDDMPG
                     IDLVASTEFGPGENPPDVILMGGAGPEYDHLTLSRVYDWMAQGVPVVAMHRSTSWNTT
                     EGLRIDTGMYLIGMEETSGRKATAVGKPAPAGFLAAAGRLGVDPEQMYMIGDDLNNDV
                     LAAQVVGMTGVLVRTGKFRQDTLDRWAADEFAMQPNQVIDTVAELPGLLGL"
     gene            24247..>25298
                     /gene="groEL"
                     /locus_tag="VF874_18685"
     CDS             24247..>25298
                     /gene="groEL"
                     /locus_tag="VF874_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9178007.1"
                     /translation="MSKIIAYDEEARRGLERGLNALADAVRVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     LRNVAAGANPMALKRGIEKAVEKITETLLKEAKEVETKEQIAATAGISAADPAIGELI
                     AEAMDKVGNEGVITVEESNTFGLQLELTEGMRFDKGYISGYFVTDPERQEAVLEDPYI
                     LLVSSKISTVKDLLPLLEKVMQSGKPLAIIAEDVEGEALATLVVNKIRGTFKSVAVKA
                     PGFGDRRKAMLGDIAILTGGQTVSETVGLKLDNVDLDVLGRARKVVVTKDETTVVDGG
                     GDAEQISGRVNQIRAE"
BASE COUNT         4215 a         8396 c         8669 g         4018 t
ORIGIN      
        1 cgccggcgat tgcggcgcgt gcacggtgtg gctcgacggc aatccggtgc acagctgcat
       61 cacgcctgcg ttccgcgccg acggtcgcga ggtgaccacg gtcgagggcc taggttcccc
      121 ggaccagatg tcgccgctgc agcggcagtt gcgtgatgcg cccggcttcc agtgtgggtt
      181 ctgcacgccg ggcatgatca tgaccgcggc gtcattctcg gacgagcaga agatggacct
      241 gccgcgctcg ctgaagggaa acctgtgccg ctgcaccggc tatcgcgcga tcgaagatgc
      301 catccgcggg gtcgccggca tcgaggacgc cgaggccggc aaggcggtgg gcgtcagcgt
      361 cggcgccccc gcgggcaccg aagtcgtcac cggcacagcc gaatacacga tggacacgca
      421 catcgacggc atgttgcatc tgaaggtgtt gcactcgccg catgcccacg cgcgcatccg
      481 atccatcgac aagacggcag cgctcgcggt gtccggcgta caccgcgtct acacgtggga
      541 agacgtgccg cgcaagatct acagcaccgc gatccacacc gatcacctcg tcgatcccga
      601 cgacacgtac atcctcgacg acgtggtgcg cttcgtcgga cagcgcgtag tggccgtggt
      661 agccgacacg atcgccgctg ccgaggaagg ctgccgcaag gtggtggtcg actacgaggt
      721 gctgctggct gtgttcgacc cggaactggc gatggcggac ggcgcgccgc agctgcacgg
      781 gtccaccgac ccgttcgtgc acgacccgat ccgcaacatt ctcgtggaac tgcacggcga
      841 aatcggcgac gtggagacgg gattcgccga ggccgacgtg atccacgagg gcacctactt
      901 ctcgccgcgc gtgcagcacg tccatttgga gacgcacggc tcgatcgcgt ggatggccga
      961 cggccggctg aacgtacgca ccagctcgca gtcgccgtcg atcgccaagc agaaacttga
     1021 gtatctgttc gacctgcgtc ccgaccagct gcgggtcttc tgcaagcggg tcgggggcgg
     1081 gttcggcggc aagcaggagc tcatcgctga ggacctcgtc gcgctggccg cgcaggacat
     1141 cggccgcccg gtctgctggg agtacacccg cgaagaggag ttcaccaccg cctcgccccg
     1201 gcacccgatg aagatcaccg tcaagctcgg cgcgcgcgcc gacggcacgc tcaccgccct
     1261 gcagttccgc aacgtgtcga acaccggcgc gtacggcaac cacggcggcg agacgctgtt
     1321 cgccggtgcg gccgccgtcg cgctctaccg ttgccccaac aagaagttcg acgggtattc
     1381 ggtgtacacg aacaccgtgc ccagcggcgc gctgcgcggt tacggcatga cgcagcccgc
     1441 cttcgcggtg gagtcggcga tccatgaact cgccgtcgcc ttggacatgg atccccttga
     1501 gctgcgccgc cgtaacatcg tgcggcccgg cgactcgttg ctggcgattc acgacgagcc
     1561 cgacgacgtc gagttcaccg aggacggtct ggctcagtgc atcgacctcg tcgatgacgc
     1621 gctgcgccgc aacgctgcgc acgcgaggag caattgggac acagccggac aaacgttggg
     1681 tgacgactgg ctcgtcggcg ccggcaccgc gagctcgctg catgagaccg ccccgcccac
     1741 cgagcacatc tccgaggcgt gggccacgtt gggcgatgac gacatctacg agatcgcggt
     1801 gggcacagtc gaattcggcg aaggcacgtc caccgtgcac gtgcagctcg ccgccaccgt
     1861 gctgtgcacc acgccgtcgc gggttcggct ggtgcagtcc gacaccgacc gcaacgggtt
     1921 cgacaccggc gcgttcgcca gcgccgggat gttcgtcgcg ggcaacgcgg tgctgcgagc
     1981 cgccaccgcg ctgcgcgacc gcatcctgcg attcgccgcc tcgcacagca acagtcatgt
     2041 atctgcgtgc gtaatggacg acgacgcggt gcggtgcggc cacacccggg ttccgttgag
     2101 cgaactcgtc gccgccgcgc ggcagcgggg catccggttc accgaatccc gcaaggccta
     2161 cgggtctccg cgcagcgtca cgtcgaacac ccacggcttc cgcgtcgccg tgcaccgggt
     2221 caccggcgag atacgcgtcc tctacagcgt gcaggccacc gacgccggcg tcgtcatcaa
     2281 cccggtgcag gtgcgaggtc aggtggaagg cggtgtcgcc caaggcatcg gcttcgtgct
     2341 gaccgagaac caccacgtcg acggcaacgg cgtcatgacc aacccgagcc tgcgcaacta
     2401 ccgcatcccg accttcgccg acgccccccg caccgaggtg atcctggtcg actcgaccga
     2461 ctcggtgggg ccgatgcgcg ccaagggcat ggccgagtgc tgcaccaacc cggtcgcgcc
     2521 tgcgctggcc aacgccgtcc acgacgccac aggggtgcgc ttccgcgaac tgccgctaac
     2581 cccggaacgg atctacgacc ggctgccgtc acgtgagctt gcgaagcgat gagcgccggc
     2641 aacgtcaccg acaacgccgc gaccgtcatc atcggtcagc gcctcaaacc cggatgcgag
     2701 gaggcgttcc tggcgtggca gcacgacttg aacagcaccg cttcccgtta cccgggattc
     2761 gttgccgcgg aggtcactcc accgactgcg gtgcaacctg actggaccgt cgtctaccgc
     2821 ttcgacacga tcgcgaactt gcgggcctgg ctgaacagcg ccacccggca ggatcggctg
     2881 cctgaggggc ggccgttttt cgaagccccg cccacccagc aggtgatccg gggcagtgcc
     2941 aaggcgcccg acgaactcgt caccgtcgtc gtgacccacc gtgtcgcccc cgagcacctg
     3001 caggagttcc tgcagtggca ggaccgactg cgagaagccg agagcaggtt tcccggtttt
     3061 cgtggttcgg agctgttccg ccctatcccc ggtgtccaag acgactggac cgcgatgtac
     3121 cgttacaaca cggccgccga cctcgatgca tggctgaaat cacctgagcg gcaacaactc
     3181 ctggccgaag gcaagaactt cgaggatttc gagttgcgca cggtcgacaa ctcgttcggc
     3241 agctggttcg cgttcgacga cggcgcagcc gacgcgaagc cgccgtcgga cttcaagacg
     3301 tcgatagccg tctgggtcgg cctctatccc actgtggttc tgctgaccct cgcaacgtca
     3361 ccgctgaaaa tgcccttgtg gcttggcatg ttggtgggca atctgttgtc cagcttcgca
     3421 atgactttcg tgacgatgcc gttttacgtc aacccactat tgaagaagtg gctgcggctg
     3481 ccggacggtg tgccgaagac tcgggtgaac gtgcgtggcg tcgtgctgat cgccgcggtg
     3541 atggcggcct ggacgctggc tttctatctc attaccaggg tgttctggca cctgccctga
     3601 cgtctatcgg ccgcccaaag ggacaggccc gttcacaccg gcgctggccg gcaatctggt
     3661 taacacgttc ggcgtcaacg tgttcgcctg cggatccgca cgagcgagcc cgaacttgac
     3721 gacttcggca ccctgtgggc ccgcgccgct gaccaggaga tcggcgcccg acgtcgtgct
     3781 ggtggtcgcc accgtgacgc cgggagcgcc accggtgaac ggattgaacg aggcggtctg
     3841 acggaacatg acatgccccg agtgttgatc cgggctgtgc gtatacatcg cgggttggcc
     3901 atccaggcga gaacccgccg accacacccg cacggtgccg tccccggcca gctggccggt
     3961 gatgatgctc tttgcaccgc cttcttgtcc ggcgacccat cccgtcgaca gcgagacgcc
     4021 tccggtgtag gactcgtcgt aggccaacaa ctccgcggac atcgccggct caccagggcc
     4081 gatctgctgc tgcggcgcgc catttgccag cgccctcgcg gtcggcgtgt acaggtcgta
     4141 acggaacgtc ctgactttcg caggctcccc cggccccgga gcggtcacga tgctctctcg
     4201 gcccgtctcc atctcgacca gacccgtcgc gatcgtcacg cctgcctgcg atccggggta
     4261 gggcttgaag ctggagaaca cgtcgggtgc aatgcccttc tcggtcggta gcttcgacga
     4321 gaaaaccttg acttcggact ccatgcccgg tccggatccc acgatgatgt tggcggccaa
     4381 cgcatttccg tcgatgttgg cacccgcgac gttgacgccg ccacggaagc ccgcgtcgaa
     4441 cggtgcgaac cgagtcaact ctgtcctgaa tggaccgtca ggcgtgtcgc tgccgctgta
     4501 gacaaccact tcggaatccg ttcccggccc agtgccgacg atcaggtcga gaatcatgtc
     4561 accgttgacg tcggccatgg ccaccgacgg ggtgccctcg aatgccggga acggatagac
     4621 ggtggcgatg accgtgtcgc cgttgccgtc gtgcacctgc acagtggcag gcttaccgtt
     4681 cgaccctggg accgccaccg cgtacgtcga cacctcggga atcacgttga cggtccccat
     4741 cagaccgttg tcctcgtggt tgaggcggtg gcagtggatg acgaaagtgc ctgcatactc
     4801 caagaattcg ctacgcaatg tcagcgatgc tggagccagc gcctgatcgt tcgcgtcaat
     4861 cgtcggcggg gggacgttgg cgttgtccac gccgaacggt tgtacgccgg ttttggtacc
     4921 aatgagggga ctcacgactt cctgcacttg aaagtcattc acgtggatgt gtatcgggtg
     4981 cccatcgttg ttgtagttgg tgatcgccca ttcttcgacg gagttgagtc gaggctggat
     5041 caatgggatg ttcgggaaga tgttgtcgtc gaactggtag gtgaacgcct tcggatcgtt
     5101 gttgctggcg ttcttgttgc cgaacccgcc cgagatcatc aactcccgcc ggacggcggg
     5161 tgtcatcacg gaggtatcga cgaacgacgt gaaggcgtcg aggttctgcc cgggctcgaa
     5221 tgccgtcgtc tgcccctcgc cgctatctgg ggtcacgtgt aacagggtct gggtcgggaa
     5281 ggtgaagaag ccgtcccgtg cgctgatgta cttggggtcg atggtcaccg tgcccagcac
     5341 ggccggggaa ttccttgtgc cgttgttggt gtacagcaca ccgggttcgg agattccctt
     5401 gacgccgtcc ttcggcggca tgtccaagac gaggtcaccg gatttcggca tcgtcaccgc
     5461 gatcgcgtac cgcgacgccg gcggaatctc cagataggtt ccgtcgccgt cgacgggccg
     5521 ctgcacctgc gtgtaggggt tcccgtcctg gcccacgatg gagaatttcg gatgcgtgcc
     5581 cgtcgctgtc tcggtgaatc tgaccggcat gtaggcgatg tcgctgatgt tggccagcac
     5641 ccagatctcg gtctggcccg gcttgagctt cagctccggc tggaactgcc cgttgacggt
     5701 gaactgcacg tcgcgctggt tgtcgggcag cgacgggttt gcggggacat tccttgatgg
     5761 gctggtgaag ctctggagat tctgcggaat gaactggttc tgcccgcggt ggttgtggat
     5821 ggagagcgga ccctggtacc aattcgtgaa gtattcggcg cctttggtgg tctccgagaa
     5881 gttgaccggg gcaagactcg gagagtacgt gccatcggcg agctgtgacc cttccggcgg
     5941 cttgagtgtg ctcacccact gctcccagta cgggttgttc aactggtggc cgccgccttt
     6001 tcggtcgaag acgaagttgt actgcaatgc catgacgcgg atgggaatgt tgttctgcgt
     6061 cactatcggt aggttgccgt cggggcggcc gatctccagc agaccggcga ggcccatgta
     6121 ggtctgctgc gccgtcaggg tgtgacgatg gctgtggtac caatacaagc cattcggcat
     6181 gtcggttggc acggcgtagt catatgtgtt gcccatcccg ggcggaatgt tgagcagcac
     6241 gttgtcggcg ttaccgctcg ggctcacgtg cagcccgtgg gtatgcagat tgatcggcgc
     6301 cgactctagg gcgggcgggt agatcggcac atccccgcct gccggggtca tcgctgggtt
     6361 gttgaaatcc gcgatcgaca gaccctgcaa gtcgttgtcg aagtggacga tcaaccgctg
     6421 cccgggatcg actcgcaatg tcggcgcggg gtaaaggttg tcgccttttg tcgagccgtc
     6481 tgagctcgtt cccttgatga gctcataccc gaacaccagg aagttggtga ccggttgctt
     6541 cacggtgtcg agcgcgacgg tgccctggtg tgcggacagc cgcacctcga ggacgccgtc
     6601 cttgctggaa agtttcaccg gctcgcggta cggggccggc ttgccgtccg gtcctgcgaa
     6661 gacccccgcc tgcggaccgg gcggggattc cggcttgtcc gacgagcacg agcccagcat
     6721 cagcgtcagg gccacgacca gcccgatggc catgatggac ctggcacgtc gtagtttcat
     6781 ctggcgaacc tacccgacct cgcgcaggaa gtgtcagctg tcttcccgca gttcgtcggt
     6841 ggtcagcagg tcgtcacgaa tgttctgctc gcggtaggcc agctggccga cgcggttggc
     6901 caccacgggc gcggtgatca acgtgaacat cgcggtgaga atgatcattc cgacgtcgac
     6961 gttcccgcgc agccggaccg ccgcgccgac gagcaccagc agcagcccga gcacctgagg
     7021 tttggtcgca gcgtgcatgc gggacaacgt gtccgggaag cggaccacgc cgatcgacgc
     7081 ggtcagcgcc agtgccgacc cgctgagcac aagcacagcc gtgactacgt caagcgcggt
     7141 catcgccgtc cttcgactcg acgtcgggca cccggaaccg cgctacgctc accgatccga
     7201 cgaaactgat caacgacagc gccgtgatcc cgtagacgac ggtggagtcc aggctgaacg
     7261 cggcccacgt gcccaacccg cacatcgcga ccgcgacgag ggtgtcgacg cccacgaggc
     7321 ggtctaatgt gctggggccg gccaggattc ggaatgtggt caacgcggcg gcggccgcca
     7381 gcatgacggc cgcgatcatc cagacggttg tcatgcttgc tcctcggcca ccggtcgcca
     7441 gtccttgtcg cgttcgaatg ctgcgatgag cagccgctcc agctgtgcga cctcgttgta
     7501 gaagcggttg accgcgcgag gggagccgac gtcgagcacg tggacgtaga gcaggcgccg
     7561 gtcccggtcg atctccagca ccatcgtgcc gggggtgatg ttgatggcat gcacggcgag
     7621 cacgagaacg aggtcggatt tcaccgccag cctgacgcga agcacagcgc tcatcggcgg
     7681 cggtccgggt ttgatcgcca gccacgcgac ctgaaggctg gacaccacaa gattgcggcc
     7741 gatgaccacg ttcagcttgg ccagcgacaa cgggtgcagc cggccctcga ttggcaccac
     7801 cggcagcgac agcaagaacg tgatcagcag tgccaccgcc aaaccgctga ggatgttggc
     7861 ggcagagatc gtgccccaca acaggatcca tacggcgacc agccagcaca ccgaccacac
     7921 ccgtagcgcc caccgtctgc tcatctcgtg cccaccaccg ccgtgatgta tcggtcgcgg
     7981 tcgagcatct cggcggccgc gcggtcggcg taggagaaga aaggcccggc cagcacagtc
     8041 agcgccaggc ccaccccgat cagcgccacc gtcggcgcca gcatgccgaa cggcatccgg
     8101 ccgacgtcgt cgcggtccag gaacgtgaca tcggcttcgg ggtcgtcgag cagcaccgtc
     8161 gggccggcgt tggacagggc accctccggt gcgtcggcgc gcggacgcca gaacgccttg
     8221 ctgtacacgc gggtgaccgc gtacagcgtc agcaagctgg tggtgactgc cccgccgacg
     8281 agcacccacg ccagcgccga cccctcctgg gcgccggcct gaagcagtgc gaccttgccg
     8341 aggaagcccg aaaacggtgg aatcccaccg agattgagcg caggcagcag gaacagcagc
     8401 gccaacaacg ggctggcggt ggcgagcccg ccgagtcggc gcagcgtcga cgcgccagcc
     8461 tgccgttcga tcagcccggc gaccaggaac agcgtcgtct gcacgatgat gtggtgcgcg
     8521 acgtagtaga tcgcgcccgt catgccgggg cggttggcca acgcgatgcc gaacaccatg
     8581 aagccgatgt ggctgaccaa cgtgaacgac agcagtcgtt tgatgtcgtt ctgggcgatc
     8641 gcgccgagca caccgaccag catcgtcagg agcgcggcga ccagcagcac actgtccaag
     8701 ccgccgctgg gaaaaagcag cgaatgcgcc cgaatgatcg cgtagacacc gactttcgtc
     8761 agcagtccgg cgaacacggc cgtcacgggc gcgggcgcgg tggggtagga gtcgggcaac
     8821 cacgtcgata gtgggaacac cgcggccttg atgccgaacg cgacgagcag caccgcgaac
     8881 agcgcgcttc tggtgccggg cgctacgtcg tcgagccgga cggcgagttc ggccatgttc
     8941 agtgtccctg tcgcagcgta gatcagcgcg agcgcgaaca agaagatcag cgacgcgacc
     9001 atcgagacca tcacataggc gatgcccgcg cgtactcggt ccgcgctggc gccgatggtc
     9061 agcagcacga agctcgcggt cagcagcacc tcgaagccga cgaagaggtt gaacaggtcg
     9121 ccggccagaa acgcgccgca cacccccgcc gacagcacca ggtaggtggg caagaagatc
     9181 gacaccggct ggcgctcgtc gccgtcgcgg atgccctgac cgatcgcata gaggaccacg
     9241 gccagcagca cgatcgacga caccaccatc atcagcgccg acagccgatc caccaccagc
     9301 gtgatgccca gcggcccgag ccccggcacc gtcggtcccc agccgccgac ctggaccgcg
     9361 atcgtgccgc acttgtcggt caggtagatc agcgtgacgc acaccgcgac gacggccgcc
     9421 agcgcggcca ggctgatcgt gcgctgcaga cgcggatggc gacccacgaa catggtgagc
     9481 gccgcgccga gcgcggggat cagcaccggc agcggagtga gggcggaggc gatactcacg
     9541 tgagttcttg ggccttccgc gagtgcgcgg gcaccgcgtc gagttcagcg ggttcgtcga
     9601 cgtcgagttc cggctccggg tggacgacgt cctcctcgac ctcgtcctcc gtctgctgcg
     9661 acacccgggt gtcctccggg tcgtcgggga cttcctcggc ggtggtgagc cgataggacc
     9721 ggtacgccag tgccaacacg aacgcggcca cacccatcga gatgacgatc gcggtgagga
     9781 tcatgccctg caccagcggg tcggcggtac tgctgcgtcc ggcgctggtg cgcccgaaga
     9841 tcggcgggtt gcccgacggg ccgccgacgg tcaggatcag aaggttgacg gcgttgccaa
     9901 tcaacaacag gcccaacaac attcgggtta agttgcgctc cagcagtaga tagacaccgc
     9961 tgctggtgag cacgccgatc aacaccaagg gcaccaaggg tgtggtcatc gcaacgccag
    10021 ttcgtcgtcg aggtgagcgc cgaggctgcg cagcacgtcg agaaccaaac ccaccacgat
    10081 caggtacacg ccgaggtcga aaaacagtgc ggtgacgaac ttgacgtggc ccagcaccgg
    10141 cacgtcggcc tcgatcagtg ccgatgacag caccggcgcg ccgaacgcca gcgacgccag
    10201 cgtggtgccg gccgagagcg ccagcccgac cccgagcacc ttgccgacgt cgatcggcag
    10261 cgtctcgccg agttcgtagc ggccgccggc gaggtagcgc agcaccaggg ccaatcccgc
    10321 ggtcagcccg cccgcgaacc cgccgcccgg tgtgttgtgg ccggtgaaga agaagtacac
    10381 cgacagcacc atgatcagcg ggaagatcac ccgcgtcgcg acttcgagca ccagtgaccg
    10441 atgccgaggg tcgcgcaact cgctgccgcg cagccacgtc acgtcgctgc tgacgggtgc
    10501 cgggtgcggt gcgtcggata cccggggcgc ggcgccaaac cggcggtggc ggaacaccat
    10561 tgacgcgaca ccggtcgccg cgacgagaag caccatgatc tcgccgagcg tgtcccaggc
    10621 gcggatgtcg acgaggagca cgttgacggt gttggcgccg tggccgcggt ggtatgccgc
    10681 gtcgggcagc agcgacgcga tcggggcccc ggtgcgggcc gccatcgcga acgcggccag
    10741 tgtggtgacg gtggcgccga cggcgagcgc aagcaccacg cgcggcccgc gccgacgctt
    10801 gatgttggcc gcctgcgctt cggccggcag cgcgcgcagc acgagcacga acaccacgag
    10861 tgtcagtgtc tcgacgagga attgggtcag cgccagatcc ggcgcaccgt gcagcgcgaa
    10921 gatcacgccg cacccgtagc cggtaatccc gacgagcagc acgccggcca gtcggttgcg
    10981 catcacggtg gtgccgagcg cggcggccac gatgagcagg ccgaccagca actgcagcgc
    11041 ggaatcccac agccggaact gcggccggtc gcgcgcaccg acgagcagtg cgccgacggg
    11101 cagcagcacc agcgtggaca ggatcactgc ctgggtcgcc ggaatcgagc cgcgctgtgt
    11161 aacggccgtc actttcaacg acaccatgtc agcagcccgc atgacggcgt cgtatccgcg
    11221 gtcggcgttg cccagcggaa gccggccgat cctggtgcgg cgcaaccggt ttcgcagcac
    11281 gaacgcaccg gcgccgacgc ctagcaccac cgccgacagc gccagcgcca ggccgacgcc
    11341 atgccacagc tcgagctggt aggagtgccc acccggcatc gtgtcggcgt agttgtcgag
    11401 gacgtcgtcg agccagtccg gcacgatgcc gagtgccagg ctggccgcag ccaaaatagc
    11461 cggggcgaca aggaaactcg acgacggtcg atgcatgtgg gcgacgagag agctcggctc
    11521 tgttcggccc ttgcgggcga atgctcccca gaggaaccga aggctgtaga tcgtggtgaa
    11581 cacggaggcg agcacgaccg cggccaacac gaagggcgcg gccgccccga gtgaggcgct
    11641 gtgcgccagg gtctcgaagt cggcctcctt ggcgacgaag ccgaggaacg gcggcaccgc
    11701 ggccatgctg gccgtcgcga acgcggcgat gacgaacagc gggcggcatt cgtggcccag
    11761 ccaggcgagc cggcggatgt cgcgggtgcc ggtggagtgg tcgatgatgc cgacgaccat
    11821 gaacagggcc gccttgaaca gtgcgtgcgc gaagagcagc gccaggccgg cgagcatcag
    11881 gttgccgccg ccggcgccga ccagcagggt gatgaagccg agttggctga cggtgccgaa
    11941 cgcgaggatc agcttgaggt cgtattcgcg gaccgcccgc cacccggcga gcagcagagt
    12001 gccgacgcca aggatcacga ccgtgggccg ccagggcggc gcatcggcga aaccgggggt
    12061 gagccgcgcg acgaggtaga caccggcctt gaccatcgcg gcggcgtgca ggtacgcgct
    12121 gaccggcgta ggggcggcca ttgcgccggg cagccagaag tgcagtggca ccagcgcgga
    12181 cttgctgacc gcgccgacga gcagcagcgc caggccgacg ctcgcgagcg ctccggacgg
    12241 cgggtcggcg accagctcgg acagcaggta ggtgcctgac gtgtgcccga ggacgatgat
    12301 cccgacgagc atcgccaggc cgccggccgt ggtgacgagc aacgcctgca tcgcggcgcg
    12361 gcggctggtg gctcgttcgg cgtagtgcgc gacgagcagg aacgacaaca ccgtcgtcag
    12421 ctcccagaac acgtagagca acagcatgtt gtcgctgacg acgagcccga acatcgcgcc
    12481 ggcgaacgcg acgagttgcg cggcgaagct gggcagccgc tgctcggtat ggccgtcatg
    12541 gtggtggaaa tagtcggcgc agtaaaccag cacgagcgcc ccgatgccca gcaccagcac
    12601 ggccatgatc gccgacagcg catcgaagcg gaggtcgacg ttcagggaca gctcgggcac
    12661 ccactgctgg tggacggtgt tgacgtggcc cgcttgtggc cagttcagtg cgacccagat
    12721 cagcgacgcc gccgggacga cggccagcgg gtagaacgcc atccggcccc accggtagac
    12781 cagcatgggc gccgccacgg cagcgaccgc gtgggcgagc aggatggcga gcatgtgact
    12841 actgcacggc gacgatgttg agtgcgtggt ggatacccac gaaatcggat tccttgcgcg
    12901 tgatcagagg aatccgcagc gcaactgcga ccgccgcgat gagcaggtcg aaacgccggg
    12961 gcctagggtc gcgaccaatc gcgcggacgc ttgcacacaa tgcgccgtag acgcgggccg
    13021 cactggtatc aaacgggatt ggggcgaacg tgctcgtgat ccagtggtac cgctgttctc
    13081 ggacaatatt cagcagcgga tcctctgtgt gcagcccgta cgccagttcg gcgagcgtga
    13141 tcgtgctgac cgcagccgtt gcagcaagag accccactgc ctcgccgggg aaatcgatga
    13201 ctacggatgt gtcgagcagc acccgctcat gttgcgcgtt cacggggcgc tccagcgatc
    13261 cgcgtcccgg tcgtcgacgg actcgtcgag caccttccgc tcctgcttga aacgctcgac
    13321 atcccaactc ggcagcccgc tcaacccggc ggcgatctcc gtgaccggga cgaactgcgg
    13381 ccgaactacg aacgcgatcg ggtcatgcgg aacaagttcc gcgaccggaa taccgttgcg
    13441 ggtcaccgtg aaacgttcac cgtgctcgac ccggcgcatg atggtcccgc tgtcgttgcg
    13501 cagctcgcgt tggctgattg tcgtcgccac acgggcaccg tagcacgctc gtgctacgtg
    13561 gtgtagcacg tccgcaggcc tcttgacatc aaggcataac ccggcgcata attcatcata
    13621 tgatgaattg attgagtgtg aggtgactgc catgaatgca agcgacgtga tcgtcgtcgg
    13681 cgcgggtccg accgggctga tgctggcctc tgagctcgcg atgcgcggca tagatgtgcg
    13741 acttctcgag gagcgccgcg acatcccgaa catcacgcgg gccttcggcg tgcacgcgcg
    13801 cacgctcgag ttgctcgacg cgcgtgggtt ggcagacgaa atcgtgcagc gtggacttcc
    13861 ggtaacggag gtggcccccg ccccgggagc agtgctcaac ctcaaggagc tgccaagccg
    13921 gtacccgatg gttctgatgg caccgcagag cctcaccgag cgggtgctga ccgccagagc
    13981 tgggcagctg ggcatcgaaa ttctctatgg cgcacaggtt gtcgggttgg agcagcacac
    14041 cggtggggtc tcggtgcggt tggccgacgg gaccaccgaa cgtgccaagt acgtggtggg
    14101 ctgcgacgga gcgcacagca ccgtgcgccg tctgctcggt atcggcttcg cgggcaagca
    14161 ataccagacg cacatactcc tcgccgacgt ccagctggcg cagccaccga ctgacgggct
    14221 ctcggccgcc acaagccccg acggcgtcgt gctcctggtt ccgttcggcg acggctggtt
    14281 ccgcgcgatc gcgtgggatc ggacacgtga gcaggctcca ctatcggagc ccgtcacgct
    14341 gtcggagatc agggacgcgt tcctgcgaat cggcaagacc gacttcggca tgggcgaaat
    14401 gcgatggagt tcgcgctttc tgtcggaacg gcggcaggcc gagcgctacc gcgtgggtag
    14461 gggcttcatc gccggtgacg ccgcgcacgt gcattcaccg cttggcgccc agggcatgaa
    14521 caccggcatt caggacgcga tgaaccttgg ctggaagctg gccgcggcca tcggcggaac
    14581 ggcaccggag tggctgctcg acagctacca gaccgagcgg cacgccgtcg gggcgaatgt
    14641 gctgaagctc accgacacgt tcaaccagct tgtgctcggc cgcagtctgc tgcgccgggc
    14701 gttccggcga gtcgccatca ccgtcctgct gaacgtcggc gtcaccaagc ggatgatggc
    14761 ggggcggctc agcggcatcg gtatccacta cccccgcccg cgcggcgcac atccgctgac
    14821 cggccagcgc atgcccgacg tcgactgttc gggaactcgg ctctacgagc tgatgcgcga
    14881 cggccgcttg gtgctcctaa ctcgatgcag cgtcgatgtc gggcgtaacg atgtcaccgt
    14941 cgctgtccac gccgacccgg cactgcccga ggcagtcttg gtcaggccgg acggctatgt
    15001 ggcttgggcg ggccgcgaag cagaatcagc tgcggcactg aaccattgga cgcctgcttc
    15061 accgcgacac tcaaatctgc gtggcgacac gccggcgaag gccgcagacg cctgagtttc
    15121 gagggtttct cgacgggcta ccggccggcc gcgaatgccc gcagcgactc gacctgcacc
    15181 gggtccagcg acgggcgcac cttctcccgt gccgccgcga cgtcggcggc agtgacgtcg
    15241 gccgcgtcga tcgagcgccg catcgcggtc aacgccgcct cacgcaacag cgccacgcaa
    15301 tccgcgccgc tgtagccctc caggtccgcc gcgagcgcat ccaggtcgac accggcggac
    15361 agcggtacgg atttgcctgc ggtgcgcagg atttcgcggc gcgcgtcggc gtcgggcggc
    15421 gcgacgaaca ccagccgctc cagccgtccc ggccgcagca gtgccgggtc gatcaggtcg
    15481 ggacggttcg tggcgccgag cacgacgacg tcgcgcagcg gctcgatgcc gtccagctcg
    15541 gtcagcagcg cggccaccac gcggtcggtg acgccggagt cgaagctctg accgcgccgt
    15601 ggcgccagcg cgtcgatctc gtcgagaaaa accagcgacg gggccgagtc gcgggcgcgg
    15661 cggaacaact cgcgcaccgc cttctccgac gcgccgaccc acttgtccat cagctccgca
    15721 cccttgaccg cgtgcaccga cagccgaccc gagctggcca acgcgcgcac cacgaacgtc
    15781 ttcccgcagc cgggcggtcc gtacagcagc acaccgtgtg gcggctcaat gcccaagcgg
    15841 gagaacgtgt ccggatgctg cagcggccac agcaccgcct cggtgagcgc ctgcttggtc
    15901 tccgccatgt cgccgacgtc ctcgagagtg acgtcgccga ccgccacctc ctcggtcgcc
    15961 gaccgcgaca gcgggcgaat cacagtgagc gcgccggtga agtcgtcctg cgtcagcgcc
    16021 ggtggcgtgt cgtcctcgct ggcccgcgcg gccgcccgca gcgccgcttc gcgcaccagc
    16081 gccgcgaggt cggcgcgcac gaaacccggt gtcttgtccg cgatttcgct gaggttgagg
    16141 ttctcggccg gcacgtcgcg caacaacacc tcgagcaagg ccttgcgggt cgccgcatcg
    16201 ggcagggaga gacccagttc gcgatcacac agtttcggtg atcgcagccg ggggtccaat
    16261 gcgtccggcg cctgcgacgt cgcgatgaac gccacgccgg gagtacccac cgccgtgcgc
    16321 agttcggcga gaatcagcgt cgcaacgggt tccgccgggg acggcagcag cgcgtcgatg
    16381 tcggtgatca acagcacccc gccgccgtcg cggaccttgg ccaccgcatc ggcaacggac
    16441 ttgagccgtt cctgcgcggc gagcgcgccg acgtccggac cgtcgagttc gacgagccgg
    16501 cgcccggcgc acaccgcccg caccagggtg gccttgccca cgcccgccgg accggtcacc
    16561 aggacaccga gattggcggc ggcgcccagc ttttggagca gttcgggctc gtcaagtgcg
    16621 agcctgagcc actcggtcag tcgggcagcc tgcgcctcgg cgcccttcag gtcgtcgacg
    16681 ctgatcgttg gcatgggctc gtaggcggcg gcggcgtgcg ctgtcgtcac tgcgacaccc
    16741 tcaccccatg tcaccgacga gttgggctgc acgctgaccg gcccgtccgg gtcggtgccg
    16801 gtgacggtca gcagctcgga cgtccacgtg atgcccaccg acgacgccag cgccgcgctg
    16861 gcctccgacg tcgacgtccc cggccccagg tcgcgcggca atagcgacac ggtgtcgccg
    16921 accgtcatca ccttgcccag caatgccatt cgcagcgtcg caggcgaggc tgactggcgg
    16981 gcaagcttgg aacccgacag cgtcaccgac ttcgcgccgt acaccgtcac cggcgcgagg
    17041 atcaccggac tgtcctctcg gagtcccgcg ttggacagcg tgacgtcgtc gagcaggatc
    17101 gtgccggccg gaacgtcggg tccggcaagc cctgccaccg ctgcggtggt gcgcgacccc
    17161 gtcaacgaca acgcgtccca ctctcgaata ccaagggcgg caatggcttc cgcgtgaagt
    17221 cggacgacgc cgcggcggga atcgacggct gcggtgttca gccgggcggt caaggtcagt
    17281 gaagccatgc gcgcctaacg ctgcggccga cgcagaccga gtcgcgccac cgaccgcccg
    17341 tgaggctggg cccggcgaat ctgcctgcgc agcgcccgcc gccgtttcgc cttcgggtcc
    17401 cacgcctcag ggtggttcgc cagccagcgc ttgctgcgca ccgcgaacgg gatgtggatg
    17461 acgtacgcga cgatgatcac caggatcgtg aggtagggga acttgaacgc cgacgcgacc
    17521 ccgagtgcga acagcgccag caccggggcg accatgttcg gcggcatcga gaacgtgtgc
    17581 acctttcgca tcggaatgcg gctgaccacc agcagcgaca cgccgaccaa ccacagcatg
    17641 acgacccagt cccacgtcca ccagccgttg ccgaactgga gcttcgccgc gatggggccg
    17701 atggccccga cagcgccggc gggggcgggc atgccgacga agtagttctt ggcgtacgca
    17761 ggctgatcca catccagcag cgcgttgaat cgcgccagcc gcagcacgat gcaggccgcg
    17821 tacagcacga cgacgagcca gccggcgttc gggttcggcg cgtcctgcag caggatcgcg
    17881 tagacgatga acgccggcac caccccgaag ttgacggcgt cggccagcga gtcgatctcc
    17941 tcgcccatcc gcgacgtcgc attgagcact cgggcgacgc ggccgtcgag tgcatcgagt
    18001 accgcggcga gcacgagaag cgccatcgcc cacgtggctc ggctttcgat cgacaggctt
    18061 ctgtcttggg cgtacttcac ggccgtcaac ccgagggaga ttgccgcgac ggtcatcgcg
    18121 ctgggcaaga agcgcagcgt gatgtgcgcc tgattcgtcg ctgttgtcat ggcagctcgg
    18181 caaggatcgt ttccgcgccc actgcgcgct gcccgggtat gaccttcacg gttgaaccat
    18241 cgggcaggta ggtgtcgagc cgggagccga agcggatcag gccgtaggtg tcgccgatat
    18301 ttagcttgtc gccaactttg gcgtcgcacc tgatgcgtcg cgccagcaac cccgcgacct
    18361 ggacgacgac cacgtcatgg ccgtccgggg tgcgaatcca catgctgttg cgctcgtttt
    18421 cggtgctcga ttgcggcttg tccgccgata gaaacgtgcc tggccggtgc tgcaccgcga
    18481 cgacctcacc gctgatcgga acccgctgca cgtgcacgtc gaacagcgac aggaaaatgc
    18541 tgacccgctg catcggctgg tccggcagtc ccagctccgc gggcggcacc gcggtatcga
    18601 tgatcgtgat ttgtccgtcc gccggcgcga cgacgacgcc ggcacggtct ggtggcaccc
    18661 gcgcgggatg gcggaagaac gccgcgcagg cgcccgcagc agagaggccg gcggtccgca
    18721 gccagcggtt cttcctaccg atcgcagcca ccgccagtcc cgctgacacg aacgggattc
    18781 cggcgcggtg catcggtgga acggtcgagc ggatcagctc gatcagtcgg gaggcttcgg
    18841 acccggagtc gtcagccggc aggtgcgggc gtttggccat tgggcgctat cttaagtccc
    18901 agacgtcgac ccgcgagcct tccgtcagtt cggtgacgtc ttcgccgatc tcgagcagac
    18961 agttcgccga cgcgagccag cgcaaatggt gtgacgccgg tggaccgtag gcggtgacgt
    19021 tgccgtcgtc gagcacaccg cggcggaatt gccgtttgcc ccgcggcgac gtcagcccgt
    19081 cggtgagcac cgctttgcgc agtggccggt gcggcgacgg caatcccatc gcggcccgca
    19141 gcgctgggcg cacgaacacc tcgaacgaca ccagcgcgct gaccgggttg ccgggcaggg
    19201 tgacgacgga cgtgccgctt gggctggatg tcgccggccc tgcggctccg agccggccag
    19261 cgccctgcgg catgcctggc tgcatcgcga ccttcacgaa ctcgatgccg tcgcggctca
    19321 gcgaatcctt cacgacctcg taggccccgg cactgacgcc gccagtggtg acgatcaggt
    19381 cggcgtcatt ggcgtaggtg gcgaggaccc ggcggaactc gtcgacgtcg tcgccggtca
    19441 tcggcgccgc gaccacctcg ccacccgcat cctgcaccgc ggcggcgagc atcaccgcgt
    19501 tcgattcgta aatctgcccc ggccgcagcg gctgccctgc cgccaccagc tcctcgcccg
    19561 tcgacaccac cagcacccgc tgccgcggag tcaccgccag ctcggtgaga ccgagtgccg
    19621 cggccagccc cagcacggcc ggcgtgacga cctgcccgct gcgcaacacc gtgacgcccg
    19681 cggtgacgtc ctcgccggcc cgacggatgt gctggccggg cttgggcgct ttgcgcacct
    19741 cgaccgtctc cacgccgccg tcggtgacct ccaccggcac caccgccgac gcaccggccg
    19801 gcaccggggc gcccgtcatg atccggtgcg ccgtgcctgg tttgagtgtc agcgtgtcgg
    19861 tgcggcccgc gggaatgtcc tcggcgaccg gcagcacgac agggttttcg tcggtcgcgg
    19921 ccgcgacgtc gtcggccatc accgcgtagc cgtccatcgc cgagttgtcg aaacccggca
    19981 gcgacagcgg cgcgacgacg tcgtcggcca gcgataggcc cgccgcgtcg gcgatcgcaa
    20041 ccgtgaccgc cgggcgagag cggatcaggt cggcgacaac ccgggtgtgc gcctcgacgg
    20101 atcgcatcag atcggatacg tgacgccggt cagctcctcg gacaccgtcc aaagccggcg
    20161 ctggacatcc tcgtcgtgtg agcgcttgct ggactccacc accttcgggt aaccgcgctg
    20221 ctcgccgaag ccgtcgggcc cgtagtactg gccgccctgc gcggccgggt cggtggcggc
    20281 ccgcagcgtg ggcagcgcac ccatctccgc gctctgcgcc atcagccccc acacgaattc
    20341 gaccggctgc ttgatcaatc cggggatgta gcgggtcagt tcggtgttcg aaccgccggg
    20401 gtgtgcggcg agcgcgaccg tcggtgcgcc tttgagcttg agccggcgct gcagctcgta
    20461 ggtgaacagc aggttcgcca gcttggactg cccgtacgcg ccgacgcggt tgtagttgcg
    20521 ctcccactgc aagtcgtcga aatggatctt cgccatgatc cggtggccga cgctgctgac
    20581 cgtcaccacg cgggagccgt cgatcggaag caggcggtcg agcagcaggc ccgtcagcgc
    20641 gaagtggccg aggtggttgg tgccgaactg caactcgaat ccgtccttgg tggtgccctt
    20701 gtcggtgtac atcacgccgg cgttgttgat cagcaggtcg atccgctggt gggcggcctt
    20761 gatctcctcg gcggcctcgc gaatcgcctg cagcgacgtc aggtcgagct gctgcagcgc
    20821 gacgaccgca ttcgggctct tggcggtgat ctgcgacacc gcctggttgc ccttgtcgag
    20881 attgcgcacg gcgagcacga cgtgggcgcc cttgtcggcc agcgctttgg cggtttccag
    20941 accgaggccg gtgttggctc cggtgatgat ggcgacgcgg ccggactggt cgggaatgtc
    21001 tgcggcggtc cacttgctgc tcatccatgt ctccttcgtc cgccacgtat cgtcgtaccg
    21061 gtccaagcta atccaacagg aatcggtcgt tgatcgcgtt cgtcgagccc gcccatccgc
    21121 ccagccgccg ggcgccgttg atgtgggcgc tgtccgggct gctgggctgg atcgtgccgg
    21181 tcgtcgctca ggtggtgtgg tacgtcctgc agccgtcaca cgggtggttg catctgctcg
    21241 tcgcggccgc gaccgccgtg ctggtgctgg tgagcgtttt cgtcgtcccg gtgtggcggt
    21301 atcgggtgca tcgctgggag atcagctcgc aggccgtgtt cacccgcacg ggttggctgg
    21361 tgcaggagcg gcgcatcgcg ccgatctcgc gggtgcagac cgtcgacacc catcgcgggc
    21421 tgctggaccg gctgttcgga ctggccaacg tgaaggtgac gacggcgtcg tcggccggcg
    21481 ccgtcgagat cgtggctctg gacacttctg ttgcggatcg tgtggtcgcg cagctgaccg
    21541 acatcgcggc gctgggaggc gaggacgcga cgtgagcgac tggcagcgcc tgagcccacg
    21601 catgctgctg gtgcatccgg tgcacgaggt gctgcgccag ctgcctgtac tggtcggggc
    21661 gatcttcttg ggttcttcta ccggcaacag cgcgtggcca ctggccgcgc tgtcggcggt
    21721 ggtcgggttc gggctgttgc gctggttctt cactacgtac cggatctccg atgagtacgt
    21781 agagctgcgc accggtgtgc tgcgccgtcg gtcaatctcg gtgccccgca atcgaattcg
    21841 ttcgatacag accgactcgc ggctgctgca ccggctgctc gggctgaccg tgctgcgggt
    21901 tggcaccggt cagcagtcgc gcagtgaggg cgcggtggcg ctggacgcgg tggaggccgc
    21961 gcaggcaccg cggctgcggg cgatgctgct ggccgagtcg ctggacgtcg cggagcagcc
    22021 gccggggcgg gtgctggcgc ggtggcagcc gtcgtggttg cggtatgcgc cgctgagctt
    22081 ttcgggtgtc gggctgatcg ccgcggtaat cgcgctggca taccaggtcg gcggcgtgac
    22141 gctctcggaa cgcgtcgcga aggccggcgc cgaggcggcg caccgcaccg gcatcgtggt
    22201 cgcggtcagc gtcggcgcgg ccggtgtgct gctggtctcg gttctgctgg cggtgctgtg
    22261 gtcatggctg acgtacggcg ggctgctgct gcgccgcgat gcgggcgtgc tgcatctgcg
    22321 gcacggcgtg ctgcgggtac gggagcacac gttcgacatg cgccggctgc gtggggggac
    22381 ttttcgtgag ccgctgctgg tgcggttctt gcgcggggga cggctggacg cggtgatgac
    22441 gggtgtgcac ggtgaagggg agtcgtcggt gctgctgccg ccgtgtccgg ctgctacggc
    22501 caaggcggtg ttgaccgacc tgatcgacga cgcatcggcg gtctccggtc cgctgcgcgg
    22561 gcacggtccg gcggcggcgc gtcggcgctg gacgcgggct ctggcggtgc cgttgttggc
    22621 cggaatcgcg attgttcttg cgccactgcc ggtttgggtg tgggcagtgt gggtcgcggt
    22681 cactgctgcc tgcgtgctgc tggcggtcga ccgtgttcgg tctttggggc atcgggtcgc
    22741 cgacggctgg ctggtcgccc gcaccggcag ccttgagcgc cggcgtgact gcatcgcctg
    22801 cgcggggatc atcgggtgga cggtgcggca gacgctgctg cagcggcggg ccggggtcgc
    22861 gacgttgatc gccgccaccg cggccggccg caagcactac cgggtgatcg acgtgccagc
    22921 cgggctggcg tggtcggtgg cggccgaggc gtcgccgtgg gtggctaaca gcatctgggc
    22981 gagtcgctag ccgggcgacc gccgcttact gtcgggacca tggccgtcgg cggggtgctg
    23041 ttcgacatcg acggcgtgct ggtgacgtcg tggaagccga tcgagggcgc cgccgagacg
    23101 gttcggattc tgggtgagca ccagatcgcc tgctcgtatc tgacgaacac caccacgcgc
    23161 actcgtgcgc agatcgccga ggcgctggcc gacgcgggca tcaccgtgcg tcccgatgag
    23221 gtgatcaccg cggcggcgct gacggccgag tacgtccgcc agaactaccc gcacgcgcgg
    23281 tgctttctgg tcaacagcgg cgacatcacc gacgacatgc cgggcatcga tctcgtcgcg
    23341 tcgacggagt tcgggcctgg cgaaaacccg cccgacgtga tcctgatggg cggcgccggc
    23401 ccggaatacg accacctgac gctgagccgc gtctacgact ggatggccca gggcgtgccc
    23461 gtcgtcgcga tgcaccgcag cacctcgtgg aacaccaccg agggcctgcg gatcgacacc
    23521 ggcatgtatc tgatcgggat ggaggagacg tccggtcgca aggccaccgc cgtcggcaag
    23581 cccgcgccgg ccggctttct cgccgcagcg ggccgcctcg gcgtcgaccc ggagcagatg
    23641 tacatgatcg gcgacgacct gaacaacgac gtgctggccg cgcaagtcgt cggaatgacg
    23701 ggcgtgctgg tgcgcaccgg caagttccgg caggacacgt tggaccgttg ggccgcagac
    23761 gaattcgcga tgcagcccaa ccaagtgatc gacacggtgg ccgagctgcc gggtttgctg
    23821 gggctctaga agtgccctcg gtcaggaatt gcgggcgtcg cgcttcgcct ggcgccgagc
    23881 ccctttggcc ttcgagctgg cgctctcttt gaggctccga ggtgagacca ggctccggta
    23941 taggacaccc cgaaagcctg gtagtcgggc ggggcatcgg cgcttcgcag cgtggcagga
    24001 tacttcgccg ccctcgccgg tatggcgcgg tgggggatat tgcgggctga cgagcgacat
    24061 caccgcaagc gtgccacagt gggccaacct tgcactcgat atgggcgagt gctaaaaagg
    24121 acgttggcac tcccgaccgg tgagtgctag gtcgggacgg tgagaccgag gcatcggtcg
    24181 tccgtcgcgg gcactgcgcc cgaccaccga cgcatgccat ccctaatccg gaggaaacac
    24241 ttcgcaatgt ccaagattat tgcgtatgac gaagaggccc gccgcggcct cgagcggggc
    24301 ctcaacgccc tcgccgacgc ggtaagggtg acgctgggcc ccaagggccg caacgtcgtg
    24361 ctcgagaaga agtggggcgc tcccacgatc accaacgacg gcgtgtcgat cgccaaggag
    24421 atcgagctgg aggaccccta cgagaagatc ggcgctgagc tggtcaagga agtcgccaag
    24481 aagaccgacg acgtcgccgg tgacggcacc accaccgcga cggtgctggc gcaggcgctg
    24541 gtgcgcgagg gcctgcgtaa cgtggcggcc ggtgcgaacc cgatggcgct gaagcgcggc
    24601 atcgaaaagg cggtcgagaa gatcaccgag accctgctca aggaggccaa ggaggtcgag
    24661 accaaggagc agatcgcggc gaccgccggc atctccgccg ctgacccggc catcggcgag
    24721 ctgatcgccg aggcgatgga caaggtgggc aacgagggcg tcatcacggt cgaggagtcc
    24781 aacactttcg gcctgcagct cgagctcacc gagggcatgc ggttcgacaa gggctacatc
    24841 tcgggctact tcgtgactga ccccgagcgc caggaagccg tgctcgagga tccctacatc
    24901 ctgctggtct cgtccaagat ctcgacggtc aaggacctgc tgccgctgct ggagaaggtc
    24961 atgcagtccg gcaagccgct ggcgatcatc gccgaggacg tcgagggcga ggccctggcg
    25021 accctggtgg tcaacaagat ccgcggcacc ttcaagtcgg tcgccgtcaa ggctcccggc
    25081 ttcggtgacc gccgcaaggc gatgctgggc gacatcgcga tcctgaccgg cggccagacg
    25141 gtgagcgaga ccgtcggcct caagctggac aacgtggacc tggacgtgct cggccgggcc
    25201 cgcaaggtcg tggtcaccaa ggacgagacc acggtcgtgg acggcggcgg cgacgcggag
    25261 cagatctccg gccgggtgaa ccagatccgc gccgagat
//
LOCUS       DASWBF010000509        18628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_61246, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000509 DASWBF010000000
VERSION     DASWBF010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18628
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61246"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VF874_18690"
     CDS             <1..708
                     /locus_tag="VF874_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEX9178008.1"
                     /translation="IAGRRVVDVSEPGGPVTAAVSTGHLVDVEPPAKGVVAHLRDSRP
                     LVRLRVPFTLRRTDIDDVERGSQDSDWDPVKEAAKKLAFVEDRAIFEGYPAAGIEGVR
                     QTTSNPVLTLPDDARQIPDVISQALSELRLAGVDGPYSVLLSADGYTAVSETTEHGYP
                     IREHLNRLVDGDIIWAPAIDGAFVLTTRGGDFDLQLGSDVSIGYLSHDADTVQLYFEE
                     TLTFLAYTAEASVALTP"
     gene            complement(736..1410)
                     /gene="purQ"
                     /locus_tag="VF874_18695"
     CDS             complement(736..1410)
                     /gene="purQ"
                     /locus_tag="VF874_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX9178009.1"
                     /translation="MTARVGIITFPGTLDDVDAARAARLAGAEAVSLWHADADLKGVD
                     AVVVPGGFSYGDYLRAGAIARFAPVMGSVIAAAGRGLPVLGICNGFQVLCEAGLLPGA
                     LTRNAGLHFVCRDLWLQVASTTTAWTSRYELGADLLVPLKSGEGRYVASEAVLDKLDG
                     EDRVVFRYRDNPNGSMRDIAGICSANRRVVGLMPHPEHATEPLTGPSDDGLGLFYSAL
                     DSVLAA"
     gene            complement(1407..1646)
                     /gene="purS"
                     /locus_tag="VF874_18700"
     CDS             complement(1407..1646)
                     /gene="purS"
                     /locus_tag="VF874_18700"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX9178010.1"
                     /translation="MARVVVNVMPKAEILDPQGQAIVGALNRLGHAGISDVRQGKRFE
                     LEVDDTVDDSAVAEIAQSLLANTVIEDWTVTREPS"
     gene            complement(1714..2394)
                     /locus_tag="VF874_18705"
     CDS             complement(1714..2394)
                     /locus_tag="VF874_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX9178011.1"
                     /translation="MTRVLAAVSVAAMAAVLSLLGQPVAQADPTTCPPNCDRIPAAAW
                     VDPVAIPLSPKYSWPDLADIAVPQQRPRFYFEDLCASPPALNDPRAFAVASKATVANP
                     PGQWQLQVQVLHWRGEAWYGGQLADDVVGSATTALRACQVTAPRASPSLTTDEPGRLA
                     AVLSVAGTAPSVVHQYLVSHPQSGSVVELAMWATSPPAESWPVIPDAQVLDALVAPLC
                     VAYIASCR"
     gene            2456..3109
                     /locus_tag="VF874_18710"
     CDS             2456..3109
                     /locus_tag="VF874_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9178012.1"
                     /translation="MQLTHFGHSCLLASFPTDHGAETTVLFDPGVFSHGFEGITGLSA
                     ILITHQHPDHVDTSRLPALVDGNPDAALYADPQTAAQLGEPWQPVNVGDELTVGGLRV
                     RGVGGVHAVIHPEIPLVNNISYLVGDAGHPARLMHPGDALYIPREPVDVLALPAAAPW
                     MKISEGVEYLRAVNPNSAIPIHQGIVSPEAYGIYYQRYTEMGSAEFRVLAAESATEF"
     gene            complement(3111..3815)
                     /locus_tag="VF874_18715"
     CDS             complement(3111..3815)
                     /locus_tag="VF874_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HEX9178013.1"
                     /translation="MAGQLIVSISGVRDRAVNDVDEFCAHLDARAVPVSFLVAPRLTG
                     YRLSDDGHTADWLRTRSEQGDAIVLHGYDTAATKTRRPEFAALPAHEANLRLMAADRV
                     MEHIGLRTRLFAPPGWRASAGTAQALPRNGFRLLASMGGITDLVRQTTTRAPVVGIGA
                     GFLSEPWWCRTLMLSAERIARRGGIVRIAVAAEQLSRFGPRQAMLDAIDLALMHGATP
                     AVYRWKAGRPSFVDAA"
     gene            complement(3903..4475)
                     /locus_tag="VF874_18720"
     CDS             complement(3903..4475)
                     /locus_tag="VF874_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9178014.1"
                     /translation="MSAPPRPRYHHGDLRAVILAETARLVADHGADGVSLRQLARNAG
                     VSHAAPAHHFTDRRGLFTALAAEGFWLLCDELKAARGNFADAALGYVRFAIDHPGHYA
                     VMFNRSLVDPADRELVTAQAAAGAELSQGVATLADPHAKTDPTGAELAAWSLVHGFAM
                     LWLNHAVPDDVAAADPMIAVERIARMLFDG"
     gene            4564..5010
                     /locus_tag="VF874_18725"
     CDS             4564..5010
                     /locus_tag="VF874_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178015.1"
                     /translation="MAPLLTLLVVTLTARLAGWLGWDYANGWPAAVAVGLAAMFVLTG
                     IAHFVQPLRSGLIAIVPPKLPAPGLLVTITGVLELLGAIGLLIPPTRVAAAVCLGLLM
                     LAVFPANVYAAGAKRAEHAPDTALAPRTLMQALFLAGAALVAVGSV"
     gene            complement(4966..7149)
                     /locus_tag="VF874_18730"
     CDS             complement(4966..7149)
                     /locus_tag="VF874_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003915458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9178016.1"
                     /translation="MSASPEPPQAKRVDHVREHHGDVVVDPYEWLRDKSNPEVINYLN
                     AENSYTEKLTDDLAPLRQQIFDEIKARTKETDLSVPARRGGWWYYGRSFEGKQYSVQC
                     RCPVADPNDWTPPQLDEHTEIVGEQVLLDENAEAEGHTFFALGAASVSIDGNTLAYSI
                     DVVGDERYTLRFKDLRTGERYPDEIKGIGAGVTWAADSGTVYYVTVDDAWRPDTVWRY
                     RLGEDTPAEQVYHEPDERFWLAVGRTRSDAYILIAAGSAITSEIRYGDAADPAAEFTT
                     VLPRREGVEYSIDHAVIGGENKFVIVHNDGAVNFTLVEAPVDDPSAQRTLIHHRDDVR
                     IDGVDAFADHLVVSYRREALPRIQMWEIGADGDYTPPHEISFDSPLMSAGLGANPNWN
                     SPMMRVGATSFLTPVRVYDLELATGHWKLLREQPVLGDYRRDDYVERRDWAVGSDGAR
                     IPLSIIHRRDLEFPAPAVIYGYGAYELCEDPRFSIARLSLLDRGMVFVVAHVRGGGEL
                     GRLWYENGKLLKKKNTFTDFVSAARHLVETGVTTPENLVAMGGSAGGLLMGAVANMAP
                     ELFAGILAQVPFVDPLTTILNPLLPLTVTEWDEWGNPLRDADVYFYMKSYSPYENVEA
                     KDYPAILAMTSLHDTRVYYVEPAKWVAALRHAKTDGQPVLLKTEMSAGHGGISGRYER
                     WKEVAFQFAWLLATGAGGPATLATGAAEPATLDTADRHQRRPGQE"
     gene            complement(7146..8039)
                     /locus_tag="VF874_18735"
     CDS             complement(7146..8039)
                     /locus_tag="VF874_18735"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9178017.1"
                     /translation="MRPALSDYRHLASGKVRELYEVDDEHLLFVASDRISAFDYVLAP
                     QIPDKGRILTAMSVFFFDLVDADNHLAGPPDDGRIPGEVLGRALVVRRLDMLPVECVA
                     RGYLTGSGLLDYEKTGALCGIALPPGLEEASRFDEPLFTPATKAELGQHDENISFDAA
                     VKLVGAERAEAARELTLQIYRRGADHALRNGIIIADTKFEFGVDEHGSLVLADEVFTP
                     DSSRYWPADQYRPGVRQHTFDKQFVRNWLTGPESGWDRSSGDTPPPLPPDVVDATRAR
                     YIEAYERISGLRFDDWIGSDA"
     gene            8093..8731
                     /locus_tag="VF874_18740"
     CDS             8093..8731
                     /locus_tag="VF874_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178018.1"
                     /translation="MKTRYVPYASRPVRLVGQIVSDVVVISWTVLWVWIGVAVYNAVA
                     AVAEAGRRVESGATDLAGNLDSAGQRADRIPFIGDALSSPLSAAGGAAHDIAGAGQNL
                     DTAASWLAWLLAFAVAGFPILALAMPWLALRLRFSLRKHRIVGLAATPAGVQLLALRA
                     LANRPLRRLASVSPDPVGAWRREDAYAINGLAALELRAAGVGPGALTWAQLE"
     gene            complement(8739..9104)
                     /locus_tag="VF874_18745"
     CDS             complement(8739..9104)
                     /locus_tag="VF874_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178019.1"
                     /translation="MTHQDLQSNVATVEPASLPERRAGRASTLVNWVLAVLTVPAAIV
                     VMFLAIGGVMSTAGCTGQSCTGPSSSWFGVLFYGAPVVAVLAIILSFFTARRRWGIAV
                     PLCALALLAVDGLVLAMSF"
     gene            complement(9142..10131)
                     /locus_tag="VF874_18750"
     CDS             complement(9142..10131)
                     /locus_tag="VF874_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEX9178020.1"
                     /translation="MRSRLGDVELSPDVFAAVMATGIVSVSAQDHHYRWISAALAAVA
                     VLSLAVTVVLVVVNSVVHQRCPFALGDPDVTVRLFTFVAACAVLGARFEAHPAVIWSL
                     AAVAWLGWLMLSPLAIRGMWLHRWTGLRDRAHGAWELVSVATAGLAIVTAHLALLTRD
                     RALFAVGFAAWLLAIGIYGLMTWLILWRAASAPSDDIWRPDSWILMGGLAIGTLAGDR
                     LHRAALTVVIPDWLLDAVRSMTVLTWVLATLWIAPLSYVTARHLQLKFTGAWWAMVFP
                     LGMYSGATFAMLVETGWRPFKTVSWVFFWIAFAAWALVALAAVAAAFRRARPR"
     gene            complement(10138..11556)
                     /gene="purB"
                     /locus_tag="VF874_18755"
     CDS             complement(10138..11556)
                     /gene="purB"
                     /locus_tag="VF874_18755"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9178021.1"
                     /translation="MTIPNVLAARYASDEMVAIWSPEAKIVAERRLWLAVLRAQAELG
                     VDVGPQALADYEKILDTVDLASIRERERVLRHDVKARIEEFNALAGHEHVHKGMTSRD
                     LTENVEQLQIRRSLELVHAHGVAVVARLAERAVQYRDLVMAGRSHNVAAQATTLGKRF
                     ASAAEETLIALTRLRELIDRYPLRGIKGPMGTGQDMLDLFDGDAHRLAELERRVAEFL
                     GFMHVFASVGQVYPRSLDHDVVSALVQLGAGSSSLAHTIRLMAGHELVTEGFAPGQVG
                     SSAMPHKMNTRSCERVNGLQVVLRGYASMAAELAGAQWNEGDVFCSVVRRVALPDAFF
                     AIDGQIETFLTVLEEFGAYPAVIERELNRSLPFLATTRILIAAVRAGMGREAAHEVIK
                     EHAVAVALAMRERGAEPDLLDRLAADPRLPLDRAALDDALSDRAAFIGAAGGQVEAVV
                     AAVDALVNRYPEAAKYTPGAIV"
     gene            11597..12361
                     /locus_tag="VF874_18760"
     CDS             11597..12361
                     /locus_tag="VF874_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX9178022.1"
                     /translation="MYFVGLDLAWSVNNQSGIAIVDSDGTLMHVDAKLHEDDIIDSLT
                     PFVCGDCIVAIDGPLIVTNATGQRPAERALNVDFGRFEAGAHSANTRIFAAGLRGARI
                     ADALGLDMNPNSGAPRCAIEVYPHPATISLFRLGRTLKYKKGDAAVRRAELLRLMELI
                     EGLADASPSLRVTGLPAWIELHSKVTAATRPFQLDKAEDPVDAVLCAHIAMYAHHRPD
                     DVTVYGDFDTGYIVTPTLPPGSVTDETDIRARLRRT"
     gene            complement(12554..12793)
                     /locus_tag="VF874_18765"
                     /pseudo
     CDS             complement(12554..12793)
                     /locus_tag="VF874_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(12860..13471)
                     /locus_tag="VF874_18770"
     CDS             complement(12860..13471)
                     /locus_tag="VF874_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9178023.1"
                     /translation="MRSVTAAVTPKGERRRHALVSAAAELLCEGGFDAVRHRAVARRA
                     GLPLASTTYYFSSLDDLLVHAVEYAGMKATEKLRARVAVLPRRRRGVEATADVLVDVL
                     LDDGPPAETSEELISRYEIYVACARHSWLRDVQRRIRQQRTALVVDTMERCGRIVRGD
                     RLNALMYAIDGAVVAALVDDGGNLRVTAKATLVDVIDVLAPLR"
     gene            13552..14439
                     /locus_tag="VF874_18775"
     CDS             13552..14439
                     /locus_tag="VF874_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9178024.1"
                     /translation="MMSGMSQLSRRAILRLGAASAYAFGTVLDSRPSTAVPRVTMTGV
                     GAPLAPPPLEPPAAAAPTMVTGSFVSATRGGVATNWAIARPPGQTKALRPVIALHGKG
                     SDAATVMAGGVEQGLAQAVAAGLPPFAVVAVDGGGSYWHKRASGEDSGEMVLTELLPL
                     LEGQDLDTSRVGFLGWSMGGYGALLLGGRLGPARTAAICAVSPALWTSYGATAPGAFD
                     SAEDWSANSVVGLPALASIPIRVDCGNSDPFYSATKQFIAQLPTPPSGGFSPGGHDGG
                     YWSQQLPAELTWMAPLLVA"
     gene            complement(14537..15808)
                     /gene="purD"
                     /locus_tag="VF874_18780"
     CDS             complement(14537..15808)
                     /gene="purD"
                     /locus_tag="VF874_18780"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX9178025.1"
                     /translation="MRVLVIGSGAREHALLMALRRDPEVDGLIAAPGNAGIAAVAEQH
                     DTDVTSGEAVAKLAADTAADLVVIGPEVPLVLGVADAVRAAGIACFGPSAAAARIEGS
                     KAFAKDVMAAVGVRTARSEIVDNPAHLDTALDRFGPERTEEKAWVVKDDGLAAGKGVV
                     VTADRAAARAHAAGLLDAGHPVLLESFLDGPEVSMLCVVDGETVVPLLPAQDFKRVGD
                     NDFGPNTGGMGAYAPLPWLPDELTGQIVSEIIAPVAAELVRRGCPFSGVLYAGLAITS
                     AGPAVVEFNCRFGDPETQAVLALLDSPLSRLLHAAATGRLAEQPAPRWRPGAAVTVVL
                     AAENYPGRPRVGDVIVGADQDGVLHAATGRRDDGALVSTGGRVLSIVGTGADLTAARH
                     AAYQKVSSIRLPGGHFRTDIALAAAEGRIRV"
     gene            15904..16685
                     /locus_tag="VF874_18785"
                     /pseudo
     CDS             15904..16685
                     /locus_tag="VF874_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            complement(16798..17115)
                     /locus_tag="VF874_18790"
     CDS             complement(16798..17115)
                     /locus_tag="VF874_18790"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9178026.1"
                     /translation="MAERYIEAVNNADTPALLALFAEDATLTHPVGTDHGHTEIADFH
                     MDVIFDGQVRMTLARSIEQGNVEVAQLEGTSPINEDTTVHTVDIFTLNDQGLVQTLDI
                     YYR"
     gene            17279..17881
                     /locus_tag="VF874_18795"
     CDS             17279..17881
                     /locus_tag="VF874_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9178027.1"
                     /translation="MRTHGWSGSAPGSDEEAVARIVAAASKAIDAHGADISIAEVART
                     LGVTRQTVYRYFPSTDALLTAAAMNAAKSYLDRLADHLAGITDPAEAVVEGIATTLEW
                     LPNDKHTGLLLGPDRAGAFSADVTSDVAVQFARTMLGRFDVDWVGIGFSDDDLDELAE
                     HLLRIIQSFVIDAGWPPRRGAKLRRYLRRWVGPALSVVSS"
     gene            17935..>18628
                     /locus_tag="VF874_18800"
     CDS             17935..>18628
                     /locus_tag="VF874_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9178028.1"
                     /translation="MKRLSGWDAMLLYSETPNVHMHTIKAGIIDVSGFEGTPTIEAFR
                     EILHSRLHKLDPMRFELVDIPFKFHHPMWREHVDVDLEYHVRPLRLPSPGGQREFDEA
                     IGEIASTPLDRSKPLWVMYFVEGIADGNVGVINKVHHALADGVASGNLIAHAMDLKDL
                     PDRDYYETDPPPSRSELMSAAFRDHLRQIGRIPATVSYTVQGIGRFRRSSRKLSTLTR
                     PLDPPPTFMNHAL"
BASE COUNT         3198 a         6434 c         6002 g         2994 t
ORIGIN      
        1 atcgccggtc gccgagtggt cgatgtcagc gagcccggtg gcccggtgac agcggccgtc
       61 agcaccggcc acctggtcga cgtcgagccg cccgccaagg gtgtggtcgc gcacctgcgc
      121 gacagtagac cgcttgtgcg gctgcgggtt ccgttcacat tgcggcgcac cgacatcgac
      181 gacgtggagc gcggttcgca ggactccgac tgggatcccg tcaaggaagc tgcgaaaaag
      241 cttgccttcg tggaggatcg ggcgatcttc gagggctacc cggccgccgg catcgagggt
      301 gttcggcaga ccacgtcgaa tccggtgctg acgctgcccg acgacgcccg ccagattccc
      361 gatgtaatca gccaggccct gtccgagttg cgactggccg gcgtggacgg cccgtactcg
      421 gtgttactgt ccgccgacgg ctataccgcc gtcagcgaga cgaccgagca cggctatccg
      481 atccgcgaac acctcaaccg gctcgtcgac ggcgacatca tctgggcacc cgccatcgac
      541 ggcgcgttcg tgttgaccac ccgcggcggc gatttcgacc tccagctggg tagcgatgtg
      601 tcgatcggct acctcagcca cgacgccgac acggtgcagc tgtatttcga ggagacgctg
      661 acgtttttgg cctacaccgc cgaagcgtct gtcgcgctga ccccgtaacc tcctgccgcg
      721 agcgtgctca aggagttacg ccgcgagaac cgaatccagc gccgagtaga acaacccgag
      781 cccgtcgtcg gacggaccgg tgagcggctc ggtggcgtgt tcggggtgcg gcatcagccc
      841 gaccacgcgg cggttggccg agcagatccc ggcgatatcg cgcatcgagc cgttcgggtt
      901 gtcgcggtag cggaacacca ccctgtcctc gccatcgagc ttgtcgagca cagcctcgct
      961 ggccacgtac cgaccctcgc cggatttcag tggcaccagc agatcagccc cgagctcgta
     1021 gcgcgacgtc cacgccgtcg tcgtcgacgc cacctgcagc cacaggtcgc ggcaaacgaa
     1081 gtgcaagccc gcgttgcgag tcagtgcgcc cggtagcagc ccggcttcgc acagcacttg
     1141 gaacccgttg caaataccca atactggcag cccgcggcct gccgcagcga tcaccgaacc
     1201 cattacggga gcgaatcggg cgatcgcgcc cgcccgcaag tagtcgccgt acgagaagcc
     1261 gccgggcacc acgacggcgt ccaccccctt gaggtcggcg tcggcgtgcc acaagctgac
     1321 cgcttctgca ccggccagcc gggccgcgcg ggcggcgtcg acgtcgtcga gcgtcccggg
     1381 aaaggtgatg atcccgacgc gggcggtcat gacggctccc tagtgacggt ccaatcctcg
     1441 atcacggtgt tggccaacag cgactgggcg atctcggcga ccgctgaatc gtcgacggtg
     1501 tcgtcgactt ccaactcaaa ccgcttgccc tgccgcacat ctgagatacc ggcgtgtccg
     1561 agccggttca acgcaccgac gatcgcctgt ccctggggat cgaggatctc ggccttcggc
     1621 attacgttga cgaccacccg tgccacgcgt tgctcctcgc tcatcgttgt gggatttcgc
     1681 cccaacatta ccggcggacc cgcgggcatc ggctcaccgg cacgatgcga tatacgccac
     1741 gcatagcggt gccaccagcg cgtcaagcac ctgcgcgtcg gggatcaccg gccaggattc
     1801 cgcgggtggt gaggtcgccc acatggccag ctcgacgacc gaaccgcttt gggggtgtga
     1861 gaccaggtac tggtgcacga cggaaggggc cgtgccggcc acgctgagca ctgctgccag
     1921 cctgcccggt tcgtcggtgg tcagcgacgg cgacgcccgc ggagcggtca cctggcatgc
     1981 acgcaacgcg gtcgtggccg agccgacgac gtcgtcggcg agctgaccgc cgtaccacgc
     2041 ttcgccgcgc cagtgcagaa cttgcacttg aagctgccat tggccgggcg ggttggcgac
     2101 ggtcgccttt gacgcgaccg cgaaggcccg cggatcgttg agcgccggcg ggctggcgca
     2161 gagatcttcg aagtagaacc gcggccgttg ttgcgggacg gcgatgtcgg ccaagtcggg
     2221 ccagctgtac ttcggcgaaa gcgggatcgc gaccggatcg acccacgccg cggcgggaat
     2281 gcggtcacag ttgggcgggc aggtggtggg atcggcctgt gcgaccggtt ggccgagcag
     2341 gctgaggacg gccgccatcg cggccacact gacggccgct agcactcggg tcacccacca
     2401 ccccctaagc ggccacaata ccgagcaggt gggttggcgg cgcacaatcg tcgacatgca
     2461 attgacgcat ttcggccact cctgtctgct cgcctcgttc cccaccgacc acggcgccga
     2521 gacgacggtg ttgttcgacc cgggcgtctt ctcccacggg ttcgaaggca tcaccggcct
     2581 gtcggcgatc ctcatcacgc accagcaccc cgatcacgtg gacacgtcgc ggctgcccgc
     2641 gctcgtcgac ggcaaccccg acgcggcgct ctacgccgac ccgcagaccg ccgcgcagtt
     2701 gggcgagccc tggcaaccgg tgaatgtcgg tgacgaactc accgtcggcg gcttgcgagt
     2761 gcgcggagtc ggcggggtgc atgcggtcat ccacccggaa atcccactcg tcaacaacat
     2821 ttcgtatctc gtgggcgacg ctgggcatcc ggcacggctc atgcatccgg gcgacgccct
     2881 gtacatcccg cgggagccgg tcgacgtgct cgcgctgcca gccgccgcac cgtggatgaa
     2941 gatctccgaa ggtgtcgagt acctgagggc ggtaaacccc aacagcgcca taccgattca
     3001 ccaaggcatc gtcagcccgg aggcctacgg catctactat cagcgctaca ccgagatggg
     3061 cagcgccgaa ttccgggtgc tcgcggccga aagtgccacg gagttctgag ttaggcagcg
     3121 tcgacgaacg acggccgacc cgccttccag cggtacaccg ccggcgttgc gccgtgcatc
     3181 aacgcgaggt cgatcgcgtc gagcatcgcc tggcgaggac cgaaacggct caattgctct
     3241 gccgcaaccg cgatcctgac gatcccgccg cgacgcgcga tgcgctcggc ggacaacatt
     3301 agcgttcggc accaccacgg ctcgctgaga aagccggcgc cgatgccgac caccggagct
     3361 cgcgtcgtcg tctgccgcac cagatcggtg attccgccca tgctggcaag gaggcgaaat
     3421 ccgttgcgcg gtaacgcttg tgctgtgcca gctgatgcgc gccaccctgg tggtgcgaac
     3481 aacctcgtgc gcaagccgat gtgctccatg actcggtccg ctgccatcag ccgtaggttc
     3541 gcctcgtgag caggcaaggc cgcgaactcg ggccgacgtg tcttggtggc cgcggtatcg
     3601 tagccgtgca gcacgatagc gtcgccctgc tcactacggg tgcgcagcca gtctgctgtg
     3661 tgaccgtcgt cggataaccg atacccggtc agtcgcggcg ccaccaggaa cgacaccggc
     3721 accgcgcgcg catccaggtg ggcgcagaat tcgtcgacgt cgttgacggc gcggtctcgc
     3781 acgcccgaga tcgacacgat tagctgtcca gccacacccc gagtgtggcc acgtcaagtg
     3841 tccgaacagt gtcatacacg ccgacgacag atgactattt cctctgcgcg aatgacagcc
     3901 cgctacccgt cgaagagcat ccgtgcgatc cgctcgacgg cgatcatcgg atcggccgcc
     3961 gcgacgtcgt cgggaaccgc gtggttcagc cacaacatcg cgaacccgtg cacgagcgac
     4021 catgccgcca gctccgcccc cgtgggatcc gtcttcgcgt gcgggtcggc cagtgtggcc
     4081 acaccttgcg aaagctccgc gcctgccgcc gcctgcgcag tgacgagctc gcggtcggcg
     4141 gggtccacca gcgagcggtt gaacatcacc gcgtagtggc cgggatggtc gatcgcgaac
     4201 cgcacgtagc ccaacgccgc gtcggcgaag tttccgcgcg ccgccttcag ttcgtcgcaa
     4261 agtagccaaa agccttccgc cgcaagagcg gtgaacaaac cgcgtcggtc ggtgaagtgg
     4321 tgggccggcg cggcgtggga caccccggcg ttgcgggcca gctgccgcag cgagaccccg
     4381 tcggcgccgt ggtcagccac cagacgggcg gtctcggcca ggatcactgc tcgcagatcg
     4441 ccgtggtggt accgagggcg cggcggcgcg ctcatcaccg catcgtaacg ccaaaacttg
     4501 acactgccta gatgacggcc taaattcgtt catatcttgt caatgtctag attggagcat
     4561 cccgtggccc cactcctcac gctcctcgtc gtcacgctca cagcccggct tgcaggctgg
     4621 ctcggctggg actacgcgaa cggctggcca gcggccgtcg cggtcggatt ggccgcgatg
     4681 ttcgtcctca ccggcattgc ccacttcgtc cagccgctgc gcagcggcct gattgcgatc
     4741 gtcccaccga aactgccggc acctgggctt ctggtgacga tcaccggcgt gctggagcta
     4801 ctcggcgcga tcggtctgct gatccccccg acccgggtcg cggccgcggt ctgcctgggc
     4861 ctgctgatgc tcgccgtgtt cccggccaac gtctacgcgg ccggcgcaaa gcgtgcagag
     4921 catgctcccg acactgcgct ggcgcctcgc acgttgatgc aggcgctatt cctggccggg
     4981 gcggcgctgg tggcggtcgg cagtgtctaa tgtcgccggc tcagcggctc cggtggctaa
     5041 tgtcgccggc ccaccggctc cggtggctaa cagccacgca aactggaacg cgacttcctt
     5101 ccagcgctcg tagcgtccgc tgatgccgcc atgtccggcg ctcatctcgg tcttcagcag
     5161 aacgggctga ccgtcggtct tggcgtgccg cagcgccgca acccatttcg ccggctcgac
     5221 gtagtaaacc cgggtgtcgt gcagcgacgt catcgccaga atggcggggt aatccttggc
     5281 ctcgacgttc tcgtacggcg aataggactt catgtagaag tagacgtccg cgtcccgcaa
     5341 cgggtttccc cactcgtccc actcggtgac cgtcagcggc aacaacggat tcaagatcgt
     5401 ggtcagcggg tcgacgaacg gaacctgggc gagaatacct gcgaacagct ccggcgccat
     5461 gttggccacc gcgcccatca gcagcccgcc ggcgctaccg cccatggcca ccaggttctc
     5521 cggtgtcgtg acgccggttt cgacgagatg tcgtgccgca ctaacgaagt cggtgaaggt
     5581 gttcttcttc ttcagcagct tgccgttctc gtaccacagt cggcccagct cgccgccgcc
     5641 acgcacatgg gccaccacga ataccatgcc ccggtccagc agcgacaacc gggcgatcga
     5701 gaacctgggg tcctcgcaca gttcataggc gccgtagccg taaatgaccg caggtgccgg
     5761 gaattcgagg tcgcgccggt ggatgatcga cagcggaatc cgagcgccgt cggagccgac
     5821 ggcccaatcc cgtcgctcca catagtcgtc gcggcggtag tcgccaagca ccggctgttc
     5881 gcgcagcagt ttccagtggc ccgtggccaa ctcgaggtcg tagacccgta ccggcgtcag
     5941 aaacgacgtg gccccgacac gcatcatcgg tgaattccag ttcgggttcg caccgaggcc
     6001 tgccgacatc agcggagagt cgaacgaaat ctcgtgcggc ggcgtgtaat ccccgtcggc
     6061 accgatttcc cacatctgaa tccggggcag cgcctcgcgc cgataactca ccaccaggtg
     6121 gtccgcgaag gcgtccaccc cgtcgatgcg cacgtcgtcg cgatgatgta tcagcgtgcg
     6181 ctgcgcagac gggtcatcca cgggcgcctc caccaacgtg aagttcaccg cgccgtcgtt
     6241 gtgcacgatc acgaacttgt tctcgccgcc gatcacggcg tggtcaatcg agtactcgac
     6301 gccttctcga cggggcagca ccgtggtgaa ctccgcggcc gggtccgctg catcgccgta
     6361 ccggatttcc gatgtgatcg ccgatcctgc cgcgatgaga atgtacgcat cactgcgggt
     6421 gcggccgacc gcaagccaga accgctcgtc gggctcgtgg tacacctgct ctgcgggtgt
     6481 gtcctcgccc agccggtagc gccacacggt gtccggccgc caggcgtcgt cgaccgtgac
     6541 gtagtagacg gtgccgctgt cggcggccca tgtcacgccc gcaccaatgc ctttgatctc
     6601 gtcggggtag cgctcgccgg tgcgtaaatc cttgaaccgc agcgtgtacc gctcgtcgcc
     6661 gaccacatct atggagtagg ccagagtgtt tccgtcgatg ctgacgctgg ccgcgccgag
     6721 cgcgaaaaac gtgtgtccct cggcttccgc gttctcgtcg agaagtacct gctccccgac
     6781 gatctcagtg tgctcgtcca gttgcggcgg cgtccagtcg ttgggatccg ccacggggca
     6841 gcggcattgc accgagtact gcttgccttc gaagctacgc ccgtaatacc accagccgcc
     6901 gcgtcgcgcc ggcaccgaaa gatccgtctc cttggtgcgg gccttgatct cgtcgaagat
     6961 ctgttgacgc aacggggcaa ggtcgtcggt cagtttctcg gtgtaactgt tctcggcgtt
     7021 gaggtagttg atcacttcgg gattcgactt gtcacgcagc cactcgtacg ggtcgacgac
     7081 cacgtcaccg tggtgctccc ggacgtgatc gacccgcttc gcctgcgggg gctcgggtga
     7141 cgcgctcacg cgtcagaccc gatccagtcg tcgaaccgca ggccggaaat ccgttcgtag
     7201 gcttcgatgt aacgcgccct ggtggcgtcg acgacatccg gcggcagcgg cggaggagtg
     7261 tctccgctgc tgcggtccca gccggattcc gggccggtca gccagttgcg gacgaactgc
     7321 ttgtcgaaag tgtgctgccg cacgccgggc cggtattggt cagcgggcca gtatcgcgac
     7381 gagtccggcg tgaacacctc gtcggccagc acaagtgacc cgtgttcgtc gacgccgaac
     7441 tcaaacttgg tgtcggcgat gatgattccg ttgcgcagcg cgtggtcggc gccccggcgg
     7501 tagatctgca acgtcagctc acgcgctgcc tcggcccgct cggcgcccac caacttcact
     7561 gccgcgtcga acgagatgtt ctcgtcgtgc tgtccgagtt cggctttggt ggcgggcgtg
     7621 aaaaggggct cgtcgaacct gctggcctcc tccagacccg gtggcagtgc gatcccgcac
     7681 agcgccccgg tcttctcgta gtccagcagt cccgagccgg tcaaatagcc gcgggcgacg
     7741 cattccaccg gcagcatgtc gagtcggcgc accacaagcg cgcggcccag cacctcgccc
     7801 ggaatccgcc cgtcatcggg gggccccgcc aggtggttgt ctgcgtcgac caagtcgaag
     7861 aagaacacgc tcatcgcggt gaggatgcga cccttgtccg gaatctgtgg cgccaacacg
     7921 tagtcgaacg ccgagatccg gtccgaggcg acgaacagca ggtgctcgtc gtcgacctcg
     7981 tacagctcgc gcaccttgcc actggccagg tgacggtagt cggagagggc tgggcgcacc
     8041 gcgtcagcct aacggcatcg cccgagggcc ctcgtgtgct gggatcgtcg gcatgaagac
     8101 acggtatgtg ccctatgcga gtaggcctgt gcgactggtc ggacagatcg tcagtgacgt
     8161 cgtcgtgatc agctggacag tgctgtgggt gtggatcggt gtcgcggtgt acaacgccgt
     8221 ggcggccgtc gccgaggcgg gccgacgggt ggaaagcggc gcgactgacc tggctggaaa
     8281 cctcgactcg gctggccaga gggccgacag gatccccttt atcggcgacg cgctgagctc
     8341 gccgctgagc gctgccggcg gcgccgcgca cgacatcgcg ggcgcgggac agaacctcga
     8401 cacggcggcc agctggctgg cgtggctgct cgcgttcgcg gtggcggggt tcccgattct
     8461 ggcgctggcg atgccgtggt tagccctgcg gctgcgcttc tccttgcgca aacacaggat
     8521 cgtcggcctc gccgccacac cggccggagt gcaactcctc gccctgcggg cgctggcgaa
     8581 ccggcccctt cgccggctgg cctccgtcag ccccgatccg gtaggcgcgt ggcgacggga
     8641 ggacgcgtac gcgatcaacg ggctcgccgc gctggaactg cgcgcggccg gtgtcggtcc
     8701 cggcgcgctg acgtgggctc aactcgagtg accccgggtc agaacgacat cgcgaggacc
     8761 agcccgtcca ccgccagcag cgccagagcg cacagcggca ccgctatgcc ccaccgacgc
     8821 cgggccgtga agaacgacaa gatgatcgcc aacacggcga cgacgggcgc gccgtagaac
     8881 agcacgccga accaggacga actcggacct gtgcacgact ggccggtgca gccggcggtg
     8941 ctcatcacgc cgccgatggc caagaacatc acgacgatcg cggctggcac ggtcaacacg
     9001 gccaacaccc agttgaccag cgtcgatgct cgtccggcgc gccgttccgg gaggctggcg
     9061 ggctcgacgg ttgcgacgtt cgattgcaga tcttggtggg tcatgaggcg gggtacccca
     9121 gattgaggcg cgttgacaca ctcagcgggg ccgagcgcgc cgaaatgcgg cggccaccgc
     9181 ggcgagggcg accagcgccc aggccgcgaa cgcgatccag aagaacaccc acgacaccgt
     9241 cttgaacggc cgccagcccg tttcaacgag cattgcaaac gtcgcgcccg agtacatccc
     9301 gagcgggaac accatggccc accaggcacc ggtgaatttc agctgcaggt ggcgcgcggt
     9361 cacatagctc agcggcgcga tccacaacgt ggcgagtacc cacgtcagca cggtcatcga
     9421 gcgcaccgcg tcgagcaacc agtccgggat gacgaccgtc agcgccgcgc ggtgcagacg
     9481 gtctccggcg agcgttccaa tcgccaaccc gcccatcagg atccagctgt ccgggcgcca
     9541 gatgtcgtcg ctgggtgcag acgcggcccg ccacagaatc agccaggtca tcagcccgta
     9601 gatgccgatc gccagcagcc acgccgcgaa gccgacggcg aacagcgcgc ggtcgcgggt
     9661 caaaagtgcc aagtgtgccg tcacgatcgc cagtcccgcc gtcgcgacgc tgacaagttc
     9721 ccaggcgccg tgggcgcggt cgcgcaggcc cgtccaccgg tgcagccaca tgccgcgaat
     9781 cgctaacgga gacaacatga gccatcccag ccacgcaacg gcggccagcg accagatcac
     9841 cgcggggtgg gcctcgaagc gcgcgcccag cacagcgcac gccgcgacga atgtgaacag
     9901 ccggacggtg acgtccggat cgcccagcgc gaacggacac cgctggtgca caacgctatt
     9961 cacgaccaca aggacgaccg tcaccgcaag tgacagcacc gccacagcgg ccagcgctgc
    10021 gctgatccag cggtagtgat gatcctgcgc cgacaccgac acgattccgg tggccatcac
    10081 cgcggcgaac acgtccggtg agagctcgac atcgccgagt cgagaccgca tcgcgcgcta
    10141 gacgatcgcg cccggcgtgt acttcgcggc ctccgggtag cggttcacca gcgcgtcgac
    10201 cgcggccacc accgcctcga cctgaccgcc ggcggccccg atgaacgccg cacgatccga
    10261 cagggcatcg tcgagcgccg cgcggtccaa cggcagccgc gggtctgcgg ccagtcgatc
    10321 cagcaggtcg ggttcggcgc cgcgctcgcg catcgccagc gcgacggcga ccgcgtgctc
    10381 cttgatcacc tcgtgcgccg cctcccgacc catgccggcg cgcaccgcgg cgatcaatat
    10441 tcgcgtggtg gccagaaacg gcagcgagcg gttgagttct cgttcgatca ccgccgggta
    10501 cgcgccgaac tcctcgagca ccgtcaggaa cgtctcgatt tgtccgtcga tcgcgaagaa
    10561 cgcgtcaggc aacgcgaccc ggcgtaccac cgagcagaac acgtcgcctt cgttccattg
    10621 tgccccagcc agttcggcgg ccatcgacgc gtatccgcgc agcacgacct gtagcccgtt
    10681 gacgcgttcg cagctgcggg tgttcatctt gtgcggcatc gccgacgatc cgacttggcc
    10741 cggtgcgaag ccttcggtga cgagctcgtg gccggccatc agccggatgg tgtgcgccag
    10801 cgacgacgag cccgcgccga gctgcacgag cgccgatacg acgtcgtggt caagggagcg
    10861 gggatacacc tgcccgacgc tggcgaaaac gtgcatgaag ccgaggaatt cggcgacgcg
    10921 gcgttcgagt tcggccagcc ggtgagcgtc accgtcgaac aggtcgagca tgtcctgacc
    10981 ggtgcccatc gggcccttga tgccacgcag cgggtaacgg tcgatcagct cgcgcagccg
    11041 cgtcagcgcg atcaacgtct cctcggcagc cgacgcgaaa cgcttgccta acgtggtcgc
    11101 ctgcgcggcg acattgtggc tgcgacccgc catcaccagg tcgcggtact gcaccgcgcg
    11161 ttcggcgagg cgggccacca ctgcgacgcc gtgcgcgtgc accagctcca gcgagcgccg
    11221 gatctgcagc tgctcgacgt tctcggtcag gtcgcggctc gtcattccct tgtgcacgtg
    11281 ttcgtggccg gccagcgcgt tgaactcctc gatgcgcgcc ttgacgtcgt ggcgaagcac
    11341 ccgctcgcgt tcgcggatcg acgccaaatc gaccgtgtcc aagatctttt cgtaatcggc
    11401 cagcgcctgc ggccctacgt cgacacccaa ttcggcctga gcgcgcagca ccgcgagcca
    11461 cagccgccgc tcggcgacga tcttggcctc cggcgaccag atggccacca tctcgtcgct
    11521 ggcgtagcgt gcggccagca cgttgggaat cgtcacaaag acacagctta cgggcctggt
    11581 aaggcaaggt gagcgaatgt acttcgtggg cttggacttg gcgtggtcgg tgaacaacca
    11641 gtccgggatt gcgatcgtcg actccgacgg cactctgatg cacgtcgacg cgaagttgca
    11701 cgaggacgac atcatcgatt cgttgacgcc cttcgtgtgc ggtgactgca tcgtcgctat
    11761 cgacggcccg ctgatcgtca ccaatgcgac cgggcagcga ccggcggagc gggcactgaa
    11821 cgtcgacttc gggcggttcg aggcgggcgc ccattcggcg aacacccgga ttttcgcggc
    11881 agggctgcgt ggcgcgcgca ttgccgatgc acttggcctg gacatgaatc cgaattcggg
    11941 tgcgcccagg tgtgctatcg aggtgtatcc gcatccggcg acgatttcgc tattcaggct
    12001 cggacgaacg ctgaaataca agaaaggcga tgctgcagta cggcgtgcgg aactgctgcg
    12061 actcatggag ctaatcgagg ggctcgcgga tgccagcccg tcgctgcggg tgaccggtct
    12121 gccggcctgg attgagcttc acagcaaggt cactgcggcc acgaggccgt ttcagctcga
    12181 caaggccgag gaccccgtcg acgcggtgct atgcgcccac atcgcgatgt atgcccatca
    12241 ccggccggac gacgtcaccg tgtacggaga cttcgacacg ggctacatcg tgacgccgac
    12301 gctaccgcct gggtcggtca ccgatgagac cgacattcgc gcgcgccttc gccgaacgtg
    12361 aacgtgctgc gaaaaaagtc agccgatttt cgcagtgggc gcacgctcgc ggactagtcc
    12421 ggcggctccg ccagctgcat cgggtcggtg atgccgggcg cgccaattcc cagctggcgg
    12481 ttgatcagcc acgtcagcgc ccacagcagc acaccgagca catcagccct cccgcaacga
    12541 tgtactgctg caccagcaca gcgatgttga ccaccgcgaa catcgagatg aacggcagga
    12601 tgtcttcgat cggcagaccc ggcgcccccg ccttgacgac ttccaccagc ggcgtatcac
    12661 tgtcagcgag cgtgcccacc ggcaccaggg ccaccgcaac tatcgacacc agcacgtaca
    12721 ccacaccagc aatagtcaag ccagacaaga ggattcgcgg aaagatacgg gcgaagtcct
    12781 tggtttcctc agccccatca cccgcttgag ttccggctgt gacccttgtt tgacggccga
    12841 atcggccacg gtcgcgcccc taacgcagcg gtgccagtac gtcgatcaca tccaccaatg
    12901 tggccttcgc ggtgacccga agattgccgc cgtcgtcgac gagcgcggcc accaccgctc
    12961 cgtcgatcgc gtacatcaac gcattgagcc ggtcaccgcg cacgatgcgg ccgcaccgct
    13021 ccatcgtgtc gaccaccaac gctgtccgct gctggcggat tctgcgctga acgtcgcgca
    13081 gccacgagtg ccgcgcacac gccacgtaaa tctcgtaccg agagatcagc tcctcgctgg
    13141 tttcggcggg cggtccgtcg tcgagcagta cgtcgacgag tacatcagcg gtcgcctcta
    13201 cgcctcgccg tcgccgcggc aaaacggcca cccgggcgcg gagtttctcc gtcgctttca
    13261 tgcccgcgta ctcgaccgcg tggaccaaca agtcgtcgag cgacgagaaa tagtacgtgg
    13321 tcgacgccag cggtaacccg gctcgccgag cgactgcgcg gtgacgcact gcgtcgaagc
    13381 ccccctcgca cagcaactcg gcggccgcgc tcaccaaggc gtgccgccgg cgttccccct
    13441 tcggggtgac cgcggctgtc acacttctga tgctgccagc cgcatggtcg gtgtactgca
    13501 gtttcgccga agtgtcagcg gctgaagcaa ttggcctgaa cggccgatgg catgatgtcc
    13561 ggcatgtcac agctaagccg tcgcgcaatt ctgcgactcg gcgcggcaag cgcgtacgcg
    13621 ttcggcacgg tcctggactc gcggccgtcc acggccgtgc cgcgcgtgac gatgaccggc
    13681 gtcggcgcgc cgctggcccc gcccccgttg gagccgccgg cagcggccgc cccgacgatg
    13741 gtcaccggat cgttcgtgtc ggcgacccgc ggcggcgtcg ccaccaactg ggcgatcgcc
    13801 cggccgcccg ggcagacgaa ggcactgcgc ccggtgatcg ccctgcacgg caagggcagt
    13861 gacgcggcaa ccgtgatggc gggcggcgtc gagcagggcc tggcgcaggc ggtggccgca
    13921 ggcctgccgc cgttcgcggt ggtggccgtc gacggcggcg gcagctactg gcacaagcgg
    13981 gcgtcggggg aggactccgg cgaaatggtg ctcaccgaac tgctgccgct gctggagggc
    14041 caggacctcg acacgtcccg cgtcgggttc ctgggctggt cgatgggcgg ctacggggcg
    14101 ctgctcctcg gcggccgcct ggggcccgcc cgcaccgccg cgatctgcgc ggtcagccct
    14161 gcgttgtgga cgtcgtacgg cgcgacggcg cccggggcgt tcgacagcgc cgaggactgg
    14221 tcagccaact cggtggtcgg actgcctgcg ctcgcgtcga ttccgatccg ggtcgattgc
    14281 ggcaacagcg atccgttcta ctcggcgacg aagcagttca tcgcccagct gcccacacct
    14341 ccgtcgggcg gcttctcccc tggcgggcac gacggcggct actggagcca gcagctgccg
    14401 gccgagttga cgtggatggc tccgctgctg gtcgcctagg gacctgctaa ttgcggcgct
    14461 gcggagctct ttcctgggcg actaccttgt tgcccgacta tcccgccgaa ggcctccgcc
    14521 atcgttcagc gggctcctag acccgaatcc ggccctcggc ggccgccaac gcgatgtcgg
    14581 tgcgaaagtg cccaccaggc aagcgaattg agctgacctt ctggtacgcg gcgtggcgcg
    14641 cggcagtcaa gtccgcaccc gtgcccacta tcgacaacac cctccccccg gtcgacacca
    14701 gtgcgccgtc gtcacgccgt ccggtcgccg catgcagcac accgtcctgg tccgcgccga
    14761 cgatgacgtc acccacgcgc ggccggccgg gatagttctc cgcggcaagc accaccgtca
    14821 ccgcggcgcc gggacgccac cgcggcgccg gctgctcggc cagccggccc gttgcggcgg
    14881 cgtgaagcag ccggctcagc gggctgtcca gcagcgcgag caccgcctgc gtctccggat
    14941 cgccgaagcg gcaattgaat tcgacgaccg cggggccggc cgaggtgatc gccaggccgg
    15001 cgtagagcac cccgctgaac gggcagccgc ggcgcaccag ctccgcggcc accggcgcga
    15061 tgatctcgga gacgatctgg ccggtcagct cgtcgggcag ccacggtagc ggcgcgtagg
    15121 cacccatgcc gccggtgtta ggcccgaagt cgttgtcgcc gacccgtttg aagtcctgcg
    15181 ctggcaacag gggtaccacc gtctcgccgt cgacaacgca cagcatcgac acctcgggcc
    15241 cgtcgaggaa ggactccagt agtaccgggt gaccggcgtc gagcaatccg gcggcgtgcg
    15301 ctcgcgccgc ggcacgatcg gccgtcacaa ccacgccctt accggcggcc agcccgtcgt
    15361 ccttgaccac ccacgccttt tcctcggtgc gctcggggcc gaaccggtcc agtgccgtgt
    15421 cgagatgcgc tgggttgtcg acgatctcgc tgcgggcggt ccgaacgccg actgccgcca
    15481 tcacgtcttt ggcgaacgcc ttcgaccctt cgatccgggc ggcggccgct gacgggccga
    15541 agcaggcgat tcccgcggcg cgcactgcgt ccgccacgcc gagcaccagc ggcacctctg
    15601 gtccgatgac caccaggtcg gcggccgtgt cggcggccag cttcgcgacg gcctcgccgg
    15661 aggtgacgtc ggtgtcgtgc tgctcggcaa ccgcggcgat gccggcgttg ccgggcgcgg
    15721 cgatcaggcc gtcgacttcg ggatcgcgcc gcagcgccat caacaacgca tgttcacggg
    15781 cacccgagcc gatcaccaga acgcgcacag cagccaacca taacggcttg cgtcgggaaa
    15841 acccggccat acacttagct gctagatgta tggtctaggg aaagcgcaca caaggagttt
    15901 gcgatgagcc gctgtccctt cggccagggt ttcgacttca ccgaccccga cctgctcctg
    15961 gcgggcatgc cggtcagcga gttcgccgaa ctgcgcaaga cggcgccggt ctggtggaac
    16021 gagcagccgg ccggatctac cacgacgcgc cggcgcacac ccggctgcgc aagctcgtgt
    16081 ccaggctttc acaccccgcg cggtcgccgc ccaggaggag aagctcgcca aggcggctca
    16141 cgaaatcgtc gtgacggcca cagagaaggg gagttcacgt tcttcgtgat tctgctcgcc
    16201 gtggcgggca acgagaccac ccgcaatgcg atgacgcaca gcatgaacgc gttcttcgac
    16261 aaccccgacc agtgggagct gttcaagcgc gaacgccccg aaacagcggt cgacgagatc
    16321 atccggtggg cgacgccggt gcatgcttcc agcgaaccgc actggcagac aacgacatcg
    16381 gcggcgtcac gatccggaag ggacaacggg ccggcgtgtt ctacagctca gccaacttcg
    16441 acgaagccgt cttcgaccaa ccgttcgagt tcaacatcct acgaaatcca aacccgcact
    16501 tggccttcgg tggcaatggc acgcactatt gcatcggcgc caacctggca cgcatggaga
    16561 tcaagttgat cttcaacgag atcgccgaca tcatgccaga cgtcaccaag ctggcggagc
    16621 ctcagcggct aaggtcgggg tggatcaacg gcgtcaagga gctcatggtc tcctacaggc
    16681 cgtgaagtac tccgtcgcta ccgcgccgaa aacaacctgc ccgcagacga tttcgcggtg
    16741 ctcgcaccgc tggtcgatgc attcactcct gccgactacg agcgcgcgga ctgcggctca
    16801 gcggtagtag atgtcgagcg tctgaaccag gccctggtcg ttcagcgtga agatgtcgac
    16861 cgtatgcacc gtcgtgtcct cattgatcgg gctggtgcct tccaactgcg ccacttcgac
    16921 gttgccctgc tcgatgctgc gagcgagcgt catgcgcacc tgtccgtcga agatcacgtc
    16981 catatgaaag tcggcgattt cggtgtgccc gtggtcggta cccaccggat gcgtcagcgt
    17041 ggcgtcctcg gcgaaaagcg ctagcaatgc tggggtatcg gcgttgttga cggcctcgat
    17101 gtatcgttcg gccacgctca tgactcctcc agtcccgaag gcatgccgtc caccgcggca
    17161 actccatcac cagcacctaa tgtagctgga caactgtcgg cgtaaagcac tgatgccaac
    17221 ttgcgaccat acgtcactac gttcgatgta tggtctacag aatgaggagc ggagtctgat
    17281 gcgcacgcac ggctggtccg gctccgcacc gggcagcgac gaggaggcgg tcgcacggat
    17341 cgtcgccgcg gcgagcaagg ccatcgacgc ccacggcgcc gacatcagca tcgccgaggt
    17401 ggcccgcaca ctcggcgtga ccagacagac cgtctatcgc tacttcccga gcaccgacgc
    17461 cctgctcacc gccgccgcaa tgaacgcggc caagagctac ctcgaccggt tggccgacca
    17521 cctcgcgggc atcacggacc ccgccgaggc cgtggtcgaa ggcatcgcga ccacgctgga
    17581 gtggctgccg aacgacaaac acacgggtct gctgctcggc cccgaccgtg ccggcgcgtt
    17641 cagcgcggac gtcacctccg acgtcgccgt gcagttcgcg cgcaccatgc tcgggcgctt
    17701 cgacgtggac tgggtcggca tcggattcag cgacgacgac ctcgacgaac tcgccgaaca
    17761 cttgctgaga atcattcagt cgttcgtgat cgacgcgggc tggccgccgc gaagaggcgc
    17821 gaagctacgg cggtacctgc gccgctgggt gggtccggcg ctcagcgtgg tcagcagcta
    17881 acgcgtgttc gtcgaagtag tcctcgccgc gccgaatggc ggttatatag gggcgtgaag
    17941 cgactcagcg gctgggacgc gatgctgctc tatagcgaaa cgcccaacgt gcacatgcac
    18001 acgatcaaag ccggcatcat cgacgtcagc ggattcgagg gcacaccgac catcgaggcg
    18061 ttccgcgaaa ttctccatag ccggctacac aagctcgacc cgatgcgctt cgagttggtc
    18121 gacatcccgt tcaagttcca ccacccgatg tggcgtgagc acgtcgacgt cgatctcgag
    18181 taccacgttc ggccactgcg gctgcccagt cccggaggtc agcgcgaatt tgacgaggcc
    18241 atcggcgaga tcgcgagcac accactggat cgcagtaagc cgctgtgggt gatgtatttc
    18301 gtcgagggca tcgcagacgg caacgtcggt gtcatcaaca aggtgcacca cgcgctggcg
    18361 gacggcgtcg cgtcaggcaa cctgatcgcg cacgcgatgg acctgaagga cctgcccgat
    18421 cgcgactatt acgaaaccga cccgccccct tcacgatccg agctgatgag cgccgcgttc
    18481 cgcgatcacc tccgccaaat cgggcgaatt cccgcgacgg tgagctacac cgtccagggg
    18541 atcggccggt tccgtcgcag ttcgcgcaag ctatccacgc tcacccgtcc cctcgacccg
    18601 ccgccgacgt tcatgaacca cgccctca
//
LOCUS       DASWBF010000510         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1310685,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000510 DASWBF010000000
VERSION     DASWBF010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310685"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="VF874_18805"
     CDS             <1..1788
                     /locus_tag="VF874_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9178029.1"
                     /translation="QPGRIGAAQSWQTLLAIEALPLEGLQFPGSEFFTIADRVPGFDV
                     DWAVRISKTSREQAIARNTKNLRRLTEQVGERDSEVSFAQDTLSEQVLLLGEYNGHLE
                     SNDDEIEVSLCPIFAVAGTTRAQCEDGALKLAQAFERNRIKVVAPLGGQRELWAAMNP
                     GGANLRAVADFSHVTISEYAAASVPCATNAVGDVRGPIFALNLSGRRNQPVHLEWFRE
                     AARDASPSVAFAGELGAGKTWAIMTLLFHLVDIGGQFLAIDRTDQGEYASPVSTLANS
                     VVVDMMRPRWSMDPLRIFPADIGVDKAVDLLTPLFGCTADSPQGLTLGELLRPELGVR
                     SIPDMLAVLRSGIDTGGMPGGKKGLPAGWAELYRHLDYWSRKRYAAALMDPNLPPLNL
                     NAEGIVIRTNRVEVPTAEEVASGDPIDPSKVFGRAIYGLSTEIGRQAFGSNKSRMGAV
                     VMDEAYHVTSTPRGLASVTRIVRDGRKDNTALILGSHDPVSDYPAGTALDLIPIRIVM
                     RHRDESLARRSLKWLGIDPDKNPHIVTDLTKNTAPKGPNSKVIPGREGEGYLRDARGA
                     IGRIKVLGPSSQMRREAMSTTPPEHADVA"
     gene            1810..>2181
                     /locus_tag="VF874_18810"
     CDS             1810..>2181
                     /locus_tag="VF874_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178030.1"
                     /translation="MVRAGGDAPLIAAGRRLLRIVVSVVLLILAAAGVLVVAQPTAVN
                     PASSPCVAFGAGPVNNGKAVIAAGVALHIPEDGIVAGLTAAMQETGLRNLANPNVPAS
                     LAAANDGLAIDGQAVGILQQAP"
BASE COUNT          351 a          707 c          769 g          354 t
ORIGIN      
        1 cagccgggcc gcatcggggc cgcgcaatcc tggcagaccc tgttggccat cgaagcgctg
       61 cccctggagg ggttgcagtt tcctgggtcg gagtttttca cgatcgctga tcgtgtgccc
      121 ggtttcgacg tcgactgggc ggtgcggatc agcaagacca gccgtgagca agccatcgcc
      181 cgcaatacca agaatctgcg ccggctcacc gagcaggttg gtgagcgcga ttcggaggtg
      241 tcgttcgctc aggacacgtt gtccgagcag gtgctgctgc tcggtgaata caacgggcac
      301 ctggaaagca acgacgacga gatcgaggtg tcgttgtgtc cgattttcgc cgtcgccggc
      361 accacccggg cgcagtgcga agacggtgcg ctgaagctgg cgcaggcctt cgagcgcaac
      421 cgcatcaaag tggtcgcgcc gctgggtggg cagcgcgagt tgtgggccgc gatgaacccc
      481 ggcggggcca acctgcgggc ggtcgctgat ttctcgcacg tcacgatcag cgagtacgcg
      541 gccgccagtg tgccgtgcgc caccaatgcc gtcggcgatg ttcgcggccc gatcttcgcg
      601 ctgaacctgt ccgggcggcg caaccagccg gtgcacctcg agtggttccg ggaggccgcc
      661 cgcgacgcgt cgccgtcggt ggcgttcgcc ggcgaacttg gcgcgggcaa gacgtgggcc
      721 atcatgacct tgctgtttca cctggttgac atcggtggac agttcctggc gatcgaccgc
      781 accgatcagg gcgagtacgc gtcgccggtg tcgacgctgg ccaactcggt cgtcgtcgac
      841 atgatgcggc cgcggtggtc gatggatccg ctgcggatct tcccggccga catcggtgtg
      901 gacaaggccg tggacctgct caccccgctg tttggttgca ccgccgactc tccgcaggga
      961 ttgacgctgg gcgagctgct gcgacccgaa ctgggggtgc ggtcgatccc cgacatgctc
     1021 gcggtcctgc gcagcggcat cgacaccggt ggcatgcccg gcgggaagaa agggctgccg
     1081 gcgggctggg cggagcttta ccggcacctg gattactggt cacgcaagcg ctacgccgcg
     1141 gcgctgatgg atcccaacct gccgccgctg aatctgaacg ccgagggcat tgtcatccgc
     1201 accaaccggg ttgaggtgcc caccgccgag gaggtcgcca gcggtgaccc gatcgacccg
     1261 agcaaggtgt tcggtcgcgc tatctacggg ttgtccactg agatcggccg ccaagcgttc
     1321 ggctccaaca agagccgcat gggcgcggtc gtgatggacg aggcctatca cgtcacctcc
     1381 acccctcgcg ggctggccag tgtgacgcgg atcgtgcgcg acggacgcaa ggacaacacc
     1441 gcactgatcc tgggcagcca cgacccggtc agcgactacc ccgccggcac cgcgctggac
     1501 ctgatcccga tccgcatcgt gatgcgccac cgtgacgaat cgctggcccg gcggtcgctg
     1561 aagtggctgg gcatcgaccc ggacaagaac ccgcacatcg tgaccgattt gaccaagaac
     1621 accgcaccca agggccctaa cagcaaggtc attccgggcc gcgaaggtga gggctacctg
     1681 cgcgacgccc gcggggcgat cggtcgcatc aaggtgctcg gcccgtcctc gcagatgcgg
     1741 cgtgaagcga tgagcaccac tccccccgag cacgcagatg tcgcatgagc acgaacgggg
     1801 gtggctgcgg tggtgagggc gggcggtgac gcaccgctga tcgctgcggg ccggcgcttg
     1861 ctgcggatcg tggtgagcgt tgtcctgctg atcttggccg cggccggggt gctagtcgtg
     1921 gcgcagccga cggcagtcaa cccggcgtcg tcgccgtgtg tggcattcgg tgcgggaccg
     1981 gtcaacaacg gcaaggccgt catcgccgcc ggtgtggccc tgcacatccc cgaggacggc
     2041 atcgttgcgg ggttgacggc ggccatgcag gagacgggcc tgcgtaacct ggccaaccca
     2101 aacgtgccgg cgtcactggc cgcggccaat gacgggctgg cgatcgacgg gcaggcggtg
     2161 ggcattctgc agcaggcccc g
//
LOCUS       DASWBF010000511         4137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_259619,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000511 DASWBF010000000
VERSION     DASWBF010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259619"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VF874_18815"
     CDS             complement(<1..776)
                     /locus_tag="VF874_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9178031.1"
                     /translation="MIDTPIAGLVNLALRDPTLGDLARRAAANPPELALVGPAGARPF
                     VASALAQDGLLLVVTATGREADDLTAELKGVVGDAAALFPSWETLPHERLSPGVDTVA
                     ARLTVLRRLALPDDDRLGPPLRVVVTTVRSLLQPMASDLVRIEPVTLTVGTEIAFEDV
                     ISRLVELAYTRVDMVGKRGEFAVRGGILDIFPPTAEHPVRVEFWGDEVSEMRMFSVAD
                     QRSIPELAVDTVIAVACREFLLTEHVRDKAAELAENAPTRG"
     gene            complement(870..1472)
                     /locus_tag="VF874_18820"
     CDS             complement(870..1472)
                     /locus_tag="VF874_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9178032.1"
                     /translation="MTGAERRHQLIDVARSLFAERGYEGTSIEEIAQRANVSKPVVYE
                     HFGGKEGLYAVVVDREMSALLDGITSSLTRSTNNQSRLRIERVALALLAYVDERTDGF
                     RILTRDSPVGITSGTYSTLLNEAVNQVSSILASDFSRRGLDSEMAPLYAQALVGSVSM
                     TAQWWLDVREPKKEVVAAHLVNLCWNGLMHLEDDPVLHDE"
     gene            1568..1639
                     /locus_tag="VF874_18825"
     tRNA            1568..1639
                     /locus_tag="VF874_18825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1601..1603,aa:Gln,seq:ttg)
     gene            1714..3168
                     /gene="glmU"
                     /locus_tag="VF874_18830"
     CDS             1714..3168
                     /gene="glmU"
                     /locus_tag="VF874_18830"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX9178033.1"
                     /translation="MTQLPDKAVIVLAAGAGTRMQSDTPKVLHTLGGRSMLAHALHSV
                     AKVGPQHLVVVLGRDRDKLAPAVAQLADELGRPVDVAVQEEQLGTGHAAACGLNALPS
                     DFDGIVVVTSADIPLLDPDTLADLVATHRADPAAVTVLTTTLPDPTGYGRVLRTQDRE
                     VIGIVEQADATASQRLIREVNAGVYAFDLAALRSALSRLREDNAQHERYLTDVVSIVR
                     QDGLTVHAKHVDDGTRVAGVNDRVQLSALAAELNRRIVAAHQLAGVTVVDPATTWIDV
                     DVTIGRDTVVQPGTQLLGRTRVGGNCTIGPDTTLADVTVGDAASVVRTHATASSIGDG
                     AAVGPYAYLRPGTVLGADAKLGAFVEAKNSTIGTGTKVPHLTYVGDADIGEHSNIGAS
                     SVFVNYDGQTKQRTKIGSHVRTGSDTMFVAPVTIGDGAYTGAGTVIREDVPPGALAVS
                     AGPQRNIEGWVERKRPGSAAAEAARKATEDDERP"
     gene            3269..>4137
                     /locus_tag="VF874_18835"
     CDS             3269..>4137
                     /locus_tag="VF874_18835"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX9178034.1"
                     /translation="MSTDWTDNRKNLMLFAGRAHPELADQVAKELDVQVTAQTARDFA
                     NGEIFVRFEESVRGCDAFVLQSHPEPLNKWLMEQLLMIDALKRGSAKRITAILPFYPY
                     ARQDKKHRGREPISARLVADLLKTAGADRIVTVDLHTDQIQGFFDGPVDHMRAQNLLI
                     GYIKDNYSTDDMVVVSPDSGRVRVAEKWADALGGVPLAFIHKTRDPRIPNQVVSNRVV
                     GDVGERTCVLIDDMIDTGGTIAGAVKLLHDDGARDVIIAATHGVLSHPAAARLAECRA
                     KEVIVTNTLPIGE"
BASE COUNT          686 a         1422 c         1395 g          634 t
ORIGIN      
        1 cctcgggtgg gcgcgttctc ggccagctcg gcggctttgt cgcggacgtg ctcggtcagc
       61 aggaattcgc ggcaggcgac cgcgatcacg gtgtcgacag ccagttccgg aatggaccgc
      121 tggtcggcga ccgagaacat ccgcatctcg ctgacctcgt cgccccagaa ctcgacgcgc
      181 acggggtgtt cggccgtcgg cgggaagatg tcgaggatgc cgcctcggac tgcgaactct
      241 ccgcgcttgc cgaccatgtc gacgcgggtg taggccagtt cgacaagtcg ggagatgacg
      301 tcctcgaagg cgatctcggt cccgacggtc aacgtgaccg gctcgatgcg gaccaggtcc
      361 gacgccatcg gctgcagcag cgagcgcacc gtcgtgacca ccacccgcaa cggcgggccc
      421 agccggtcgt cgtcgggcag cgcgagcctt cgcagcactg tgagccgggc ggcgacggtg
      481 tcgacgccgg gtgacagccg ctcgtgcggc agggtctccc aggacgggaa cagcgcagcg
      541 gcgtcgccga ccacgccttt gagttctgcg gtcaggtcgt cggcctcgcg gccggtggcg
      601 gtgacgacca gcaggagccc gtcctgcgca agcgcggacg cgacgaacgg ccgcgcgccg
      661 gccggcccga cgagcgccag ttcaggggga ttagcggcgg cgcgccgggc gaggtcgccc
      721 agagtcgggt cgcgcagcgc caggttgacg agccccgcga tcggggtgtc gatcatggtg
      781 tggccagtct agttgcgcgc cttgctagtt gctcttcgcg caagcgctca tcggcgctga
      841 atcctcttcg cgcaagcgct catcggcgcc tactcgtcgt gcagcaccgg atcgtcctcg
      901 aggtgcatca gcccgttcca gcacaggttg acgaggtgag cggccaccac ttccttcttc
      961 ggctcacgga cgtccagcca ccactgggcc gtcatcgaca ccgagccgac gagcgcctgc
     1021 gcatacaaag gcgccatctc ggagtcgagt ccgcggcggg agaaatcgct ggccaggatc
     1081 gatgacacct gattgacagc ctcgttcagc agcgtcgagt aggtgcccga cgtgatgccc
     1141 accggggagt cgcgggtcag gatgcgaaag ccgtcggtgc gttcatcgac gtaggcgagc
     1201 agcgccagcg ctacccgctc gatgcgcagc cgggactggt tgttggtcga cctggtcaac
     1261 gacgacgtga tgccgtcgag cagcgccgac atctcgcggt ccaccaccac cgcgtagagc
     1321 ccctctttgc cgccgaagtg ctcgtagacg acgggcttgg agacgttggc ccgctgggcg
     1381 atctcctcga tcgaggtgcc ttcgtagccg cgctcggcga acagcgaccg cgcgacgtcg
     1441 atcagctggt ggcggcgctc cgcacccgtc atgcgggcgc gtggagtgcg cgtttccttc
     1501 tctagtgccg ccacgtgacc agggtaatcg gtttgtatac agtcgctacg ctgtcttcgg
     1561 tgaccggtcc gtcgtggtgt aatcggcagc acatcagatt ttggttctga gagttcaggt
     1621 tcgagtcctg gcgacggagc attattggcc gcgagcgagg gtgtttgtac gttccgcagc
     1681 ggcgtatcgg cgcagaagcg atagggaggc cccgtgacac aacttcccga caaggccgtt
     1741 atcgtgctgg ccgccggggc cggaacgcgc atgcagtcgg acactccgaa ggtcctacac
     1801 acgctgggcg gccgcagcat gctggctcac gcgctgcatt cggtggccaa ggtcgggccg
     1861 caacacctgg tcgtggttct cggccgcgac cgcgacaagc tcgccccggc ggtggcgcag
     1921 ctcgccgacg aactcggccg gcccgtcgac gttgccgtgc aggaggagca gctcggcacg
     1981 ggtcacgcgg cggcctgcgg cctgaacgcg ctcccctccg acttcgacgg catcgtcgtc
     2041 gtcacgtccg cagacatccc gctgctggac cccgacaccc tcgccgacct cgtcgccacc
     2101 caccgcgccg acccggcggc tgtcacggtg ctcaccacga cgcttcccga cccgaccggc
     2161 tacggccgcg tcctgcgcac ccaggaccgc gaagtcatcg ggatcgtcga acaggccgac
     2221 gccacggcgt cgcagcgact gatccgcgag gtcaacgccg gcgtctatgc gttcgacctc
     2281 gctgcgctgc ggtcggcgtt gagccggttg cgtgaggaca acgctcagca tgagcgctat
     2341 ttgaccgacg tcgtgtcgat cgtgcgccag gacgggctga ccgtgcacgc caagcatgtc
     2401 gacgacggca cgcgggtcgc aggcgtcaac gacagggtgc agctctcggc gctggccgcc
     2461 gagctcaacc gccgcatcgt cgccgcgcac cagctggccg gcgtcaccgt cgtcgacccc
     2521 gcgacgacgt ggatcgacgt cgacgtgacg atcggccgcg acaccgtcgt ccagcccgga
     2581 acgcagctgc tcggccgcac gagggtcggc ggaaattgca cgatcggccc ggacaccacg
     2641 ctggccgacg tgacggtcgg cgacgccgcg tccgtcgtac gcacccatgc gaccgcgtcc
     2701 tcgatcggcg acggggctgc cgtcggtccg tacgcgtatc tgcggcccgg caccgtgctc
     2761 ggcgccgacg ccaagctcgg cgcgttcgtc gaagccaaga actcgaccat cggcactggc
     2821 accaaggtgc cgcacctgac gtacgtcggc gacgcggaca tcggcgagca cagcaacatc
     2881 ggcgcgtcca gcgtgttcgt caactacgac gggcagacca agcaacgcac gaagatcggc
     2941 tctcacgtgc ggaccggctc ggacaccatg ttcgtcgcgc cggtaaccat cggcgacggc
     3001 gcgtacaccg gtgccggcac ggtgatccgc gaagacgtcc ctcccggcgc gctggccgtg
     3061 tccgcgggtc cgcagcgcaa catcgaaggc tgggtcgaac gcaaacggcc cgggtcggcg
     3121 gctgcggagg cggcccgcaa ggcgactgag gacgacgagc ggccatgatc tcgtcgcctg
     3181 aggtttgctt ttcggccacc acgtaacata aggccgacat tcccgatcca taaggccgac
     3241 attcccgatc catcaccggc gaggcaccgt gagcaccgac tggaccgaca accgcaagaa
     3301 cctgatgctc ttcgcgggcc gtgcgcaccc cgagctcgcc gaccaggtgg ccaaggaact
     3361 cgacgtgcag gtcaccgcgc agacggcacg ggacttcgcg aacggggaga tcttcgtccg
     3421 cttcgaggag tccgtccgcg gctgcgacgc gttcgtgctg caatcccatc ctgagccgct
     3481 gaacaagtgg ctgatggagc agctcctgat gatcgatgcg ctcaagcgcg gcagcgccaa
     3541 gcggatcacc gcgatcctgc cgttctatcc gtacgcccgc caggacaaga agcaccgcgg
     3601 gcgggagccg atctcggcgc ggctggtggc cgatctgctc aagaccgccg gcgcggaccg
     3661 catcgtcacg gtcgatttgc acaccgacca gatccagggc ttcttcgacg ggccggtgga
     3721 ccacatgcgc gcccagaacc tgctgatcgg ctacatcaag gacaactatt cgaccgacga
     3781 catggttgtg gtctcccccg attcgggccg ggtgcgcgtc gccgagaagt gggccgacgc
     3841 gctcggcggc gttccgctgg cgttcatcca caagacccgc gacccgcgca tccccaacca
     3901 ggtggtgtcc aaccgggtcg tcggcgacgt cggtgagcgc acctgcgtgc tgatcgacga
     3961 catgatcgac accggcggaa ccatcgcggg agcagtgaag ctgctgcacg acgacggcgc
     4021 gcgcgacgtg atcatcgcgg cgacgcatgg cgtgctgtcg catccggcgg ccgcgcggct
     4081 ggccgaatgc cgcgcgaaag aggtaatcgt caccaatacg ctgccgatcg gcgaaga
//
LOCUS       DASWBF010000512         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_569030,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000512 DASWBF010000000
VERSION     DASWBF010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569030"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF874_18840"
     CDS             <1..234
                     /locus_tag="VF874_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178035.1"
                     /translation="HAAPNQATRRGQGVTLSPSAEHSEHVEAGQYRGAVQDLTDVSNM
                     LHNHCRDTLPYPLRLAIDAGDVLNDLESVASPT"
     gene            306..980
                     /locus_tag="VF874_18845"
     CDS             306..980
                     /locus_tag="VF874_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX9178036.1"
                     /translation="MPQVLADTVTDFLGKPRARGWIHLCSAASAIVAGAALVSVAWIA
                     ASPKAGWATLIYAAAIVAMFSVSATYHRVHSRSPAAEMWMRRADHSLIFVLIAGSYTP
                     IALLAMPPRTGTQILTVVCIGAAAGVALKMLWPSAPLWLGVPLYLLLGWAAIWFTGAL
                     LDSAGVAVVALLVAEGVLYNIGAVFYGLRWPNPWPQTFGYHEFFHAFTAAAAICHYIA
                     VWVVVL"
     gene            1213..2226
                     /locus_tag="VF874_18850"
     CDS             1213..2226
                     /locus_tag="VF874_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9178037.1"
                     /translation="MRHFSNLRTITLHGDRVAYRDEGAGEVLLLIHGMGGSSCSWREI
                     VPKLAKKYRVIAPDLLGHGQSDKPRGDYSLGAFAVWLRDLLDALDIPKATVIGHSLGG
                     GVAMQFAHQHRDYCQRLVLISSGGLGTDVGRTLRILSLPGAELVLPLIASRPAIKTGN
                     ALRSWLSSDQGPTRFSETLRAHASLSNRENRQAFLRTLRSVVDHRGQAVCALNRLHFH
                     VDLPALIISGEQDRVIPVAHARAAHNALPNSRLHVIPGVWHHPQVERPDTVATLISDF
                     MTETAQPPRFASATAGASVTELPHHARRSRVPESWTTAAHARSAVRRIGGRRMRRHVR
                     LSA"
     gene            complement(2267..2479)
                     /locus_tag="VF874_18855"
     CDS             complement(2267..2479)
                     /locus_tag="VF874_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178038.1"
                     /translation="MTGTAATSLADPATCVDANGVECNVTAPGVEGTAGPGGVVGTAP
                     GVDGTAGPGGVGGCVIGIGCLDIPTG"
     gene            complement(2647..>2802)
                     /locus_tag="VF874_18860"
     CDS             complement(2647..>2802)
                     /locus_tag="VF874_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX9178039.1"
                     /translation="SAEDETTGVDFTQHAETAYAEGVHGHQPSRRPGSFGDFGGPRPQ
                     PETAEEG"
BASE COUNT          485 a          958 c          849 g          510 t
ORIGIN      
        1 catgctgcgc caaaccaggc gacacgccgc ggccaagggg tgacgctgtc gccgtcagcc
       61 gagcactccg agcacgtgga agcgggccag taccgcggag cggttcagga cttaaccgac
      121 gtgtccaaca tgctccataa ccactgccgc gataccctgc cctatccgct acggctcgcc
      181 atagacgcag gagacgtcct gaacgacctc gaatcggtgg caagtccaac gtgatcattg
      241 gacaagccga cccaaaggtg ccacctgtcg cgactcgtcg tgcggccagg ggcgctgatg
      301 atgacctgcc ccaagtcttg gccgacaccg tcaccgactt cctcggcaag ccccgcgcac
      361 gcggttggat tcacctctgc tcggcggcca gcgccatcgt ggctggggcg gcgctggtgt
      421 cggtggcctg gattgcggca tcaccgaagg ccggctgggc cactctgatt tacgccgccg
      481 ccatcgtcgc catgttcagc gtcagcgcta cctaccaccg agtgcactcg cgctcccccg
      541 ccgccgaaat gtggatgaga cgtgccgacc actcgctgat tttcgtgttg atcgccggca
      601 gctacactcc catcgcgcta ttggcgatgc caccgcgcac cggcacacag attctgaccg
      661 tggtatgcat cggcgcggcc gcaggtgttg ccctcaagat gctgtggccg tcggcgcctc
      721 tgtggctggg cgtgcccttg tacctactgc tcggctgggc ggcgatttgg ttcaccggag
      781 cgctcttgga cagcgcgggt gtcgcggtcg tcgcgctact cgtcgccgaa ggtgtgcttt
      841 acaacatcgg cgctgtcttt tacggccttc gctggcccaa cccgtggcca cagaccttcg
      901 gctatcacga gttctttcat gcgttcaccg cggccgcagc gatctgccac tacatcgccg
      961 tctgggtcgt cgtcttgtga cgacctttcg cttgccgagc gccgcaggtc gaagaacagc
     1021 tccagcaatt cctcggcgcg gcgtaagggc gtaacagcac cgccgcgata cacggcacct
     1081 ggtgtcacac ccaggcatct cttgcgtagg tgcgggccag aacacaaacc acgcagcccc
     1141 cagccgctcg gctcctcgat ccgcgattgc ccatttagaa agctccaaga catctttcag
     1201 aaacttcaga ccatgaggca tttcagtaac cttcggacga tcacactgca cggcgatcgc
     1261 gtcgcatacc gcgacgaggg tgccggcgag gttctgctgc tgatccacgg catgggcggt
     1321 agttcgtgca gctggcgaga gatagtgccg aaactggcca agaagtatcg cgtcatcgca
     1381 ccggacctcc tcggccacgg tcaatccgac aagccgcgcg gcgactactc gttgggcgct
     1441 tttgccgtct ggctgcggga tttgctcgac gcgctcgaca tccccaaggc tacggtcatc
     1501 ggccactccc ttggtggcgg cgtcgcgatg cagttcgctc accagcaccg cgactactgc
     1561 cagcggctgg ttctgatcag cagcggcgga ctcggcactg acgtcggccg tacgttacgg
     1621 attctgtcgc tgccgggagc cgaactggtg ctgccgctga ttgcgtcgcg gcccgcaatc
     1681 aaaaccggca atgcgctgcg gtcgtggttg tcgtccgacc aagggccgac ccgattcagc
     1741 gagacattgc gagcgcatgc atcgttgtcg aaccgggaga acagacaggc gttcctgcgc
     1801 acgttgcgtt cagttgtcga ccaccgcggc caggcggtct gcgcgctgaa tcgactccat
     1861 ttccatgtgg atctgccggc tctgatcatc agcggtgagc aggaccgggt catcccggtc
     1921 gctcacgcgc gcgcggcaca caacgccctg ccgaacagcc gtctgcacgt catacctggt
     1981 gtctggcacc acccccaagt cgagcgaccc gacaccgtgg cgacccttat cagtgacttc
     2041 atgactgaga cagctcagcc gccgcgcttc gccagcgcca cggccggtgc ctccgttacc
     2101 gaactacccc atcacgcgag acggtcccgg gttccggaat cgtggacgac cgccgctcac
     2161 gcacggtctg ccgttcgtcg gatcggggga cgacggatga gaaggcacgt ccgcctgtcc
     2221 gcgtgagcag ccgccaccct gtgaaatcgg ccgcagttac aggtacttag ccggtcggta
     2281 tgtcgaggca gccgatgccg atgacgcagc cgccgacacc accgggtccc gcggttccgt
     2341 cgacacctgg agcagtcccg acgacgccac ccggccccgc ggttccttcg acacccgggg
     2401 cagtgacatt gcactcgacg ccgttagcgt ctacgcaggt cgccgggtcg gcaagcgagg
     2461 tcgcggcagt gcccgtcata acagcacctg cgattgccgt cgcggcagca ccagtgagaa
     2521 gcaacttcgt cattgtttgc gatttcacta acatccatgt cgggtacctc actggccacg
     2581 tcttgaaaca gtcaaaacac cgtcgagggt gcaagcgaac agccagcgca gcgcgacgcc
     2641 gacgtctcaa ccttcttcgg cagtctcggg ttgaggacgc ggcccgccga agtcgccgaa
     2701 cgacccgggc cggcgcgacg gctgatgccc gtgcactccc tcggcgtatg cggtctcggc
     2761 gtgctgggtg aagtcgacgc cggtcgtctc gtcctcggcg ct
//
LOCUS       DASWBF010000513         4708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_714664,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000513 DASWBF010000000
VERSION     DASWBF010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714664"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VF874_18865"
     CDS             <1..559
                     /locus_tag="VF874_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HEX9178040.1"
                     /translation="QCVYVVDSAGALVLEGVRDRVAALVAELGDDAQVGFHGHENLGL
                     GVANSVEAVRAGAKQIDGSCRRFGAGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAE
                     EVVAPAMPAECLLDRNALIMGYSGVYSSFLKHAIRQSERYGVPAHQLLHRAGERKLIG
                     GQEDQLIDIALEIKREQESASRGGT"
     gene            complement(566..1084)
                     /locus_tag="VF874_18870"
     CDS             complement(566..1084)
                     /locus_tag="VF874_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178041.1"
                     /translation="MWGLTTLLSALAVLPACGGATNSSGETTPSTPATTTTTTSAVAA
                     PPTTSATRTAKWVDLDVGDCLADPPPSDPSVVTVALVDCATPHMAEVFFRGPLTVNAA
                     IPEVANPACSAQFPKYTGQSVDASELAVTFLVDSNQDRTTIDPTTGPAPSYVICLLQD
                     ANGRPLTASARR"
     gene            1212..1622
                     /locus_tag="VF874_18875"
     CDS             1212..1622
                     /locus_tag="VF874_18875"
                     /inference="COORDINATES: protein motif:HMM:NF027839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX9178042.1"
                     /translation="MIIARGNIRRGVLGVIAAGVLSGVATAIVVLPSAKGEPTPTPAA
                     DPCLASNLTGTISKVNQEISAYLQSHPETNQALADVAKQSPFSAQSAFTAYFDAHPAE
                     AADLHTLQQPLVDLSNQCGYQVTPGQVLGALSDL"
     gene            complement(1680..2585)
                     /locus_tag="VF874_18880"
     CDS             complement(1680..2585)
                     /locus_tag="VF874_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9178043.1"
                     /translation="MDLSASAGRASPPTDRAVRVLDFLSQHPQERFGVSELARRVELS
                     KPTCLGIVTTLTESGYLIRDERDKTYRLGPALITLGHRAQESMRVSPAAGEELRRLST
                     RFATTAALSAVVDDRIALLELVAPPGARVGVEVGQSYPFAPPVGLMFVLWDDEAVRDW
                     LAKEPTIPLRTDTERLHRVIAECRADGYLVERLTPGGQRLYALMAGMSNSLPDELRAL
                     LGEIVSDIGERVYLRGENSEAGQRHDVSVISAPVYDHHRRQAMVASLQIGRALTDAEI
                     TERARELVVAADALTAQLGGVTPRR"
     gene            2674..3801
                     /locus_tag="VF874_18885"
     CDS             2674..3801
                     /locus_tag="VF874_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178044.1"
                     /translation="MYTDPLTAAIAEAEELVAAAPHIETEADLLEGLQYLAQGIAACT
                     HVTFHHDRDHPFFLSGTGPFTKMGLDNPDTLYFGTRVRPDHEYVVSGVRGTTTDLSFQ
                     LLGGEYTDEGVPVSEAAFDDRELDIAADGSFEWRFTPATPAQLVVREVYGDWSQRRGT
                     IAIKRVDSAGTAPPPLTRETIEKRYAAAGKQLVNRIKTWLQFPQWFYLNLPANTLVPP
                     RLTPGGLATQYSSAGHFDLSPDQALVITIPESDAPYLGFQLGSLWYISLDYINHQTSL
                     NATQAQADPDGKIRIVVAERSPGVTNWVETLGHHKGFLQFRWQRVSRKLTEADGPTVE
                     LVDFDAVPATLPFYEHNKITDEDWRERIAQRQRAIATRMLG"
     gene            3804..4577
                     /locus_tag="VF874_18890"
     CDS             3804..4577
                     /locus_tag="VF874_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9178045.1"
                     /translation="MLEGKVVCISGVGPALGTTLARRCAEYGADLVLAARTAERLDDV
                     AKQITDTGRRAVAVATDITDDDQVKNLVDASLAAYGRVDVLINNAFRVPSMKPLANTT
                     FQHIRDAIELTVLGALRLTQGFTPALAEANGSVVNVNSMVIRHSQAKYGAYKMAKSAL
                     LAMSQSLATELGEQGIRVNSVVPGYIWGDTLKGYFNHQAGKYGTTVEQIYRATAANSD
                     LKRLPTEDEVASAIIFFAGDLSSGITGQTLDVNCGEYKA"
     gene            4577..>4708
                     /locus_tag="VF874_18895"
     CDS             4577..>4708
                     /locus_tag="VF874_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9178046.1"
                     /translation="MAERTDVGTVDDLHESASRLIGLDDFGLDDDNYREGLAVILDSY
                     "
BASE COUNT          871 a         1536 c         1514 g          787 t
ORIGIN      
        1 ccaatgcgtc tacgtcgtcg actccgccgg cgcgctggtg ctggagggtg ttcgcgaccg
       61 cgttgccgcg ctggttgccg aactgggtga cgacgcacag gtcggtttcc acggccacga
      121 aaacctcggc ctcggggtcg cgaactccgt cgaggccgtg cgcgccggtg ccaagcagat
      181 cgacggcagc tgccgccgct tcggcgcggg ggccggcaac gcgccggtcg aggcgctgat
      241 cggggtcttc gacaagatcg gcgtcaagac cggcatcgac ttcttcgaca tcgccgatgc
      301 cgccgaagag gtggtcgcac cggccatgcc tgccgaatgc ctgctcgacc gcaatgcact
      361 gatcatgggc tattccgggg tgtactcgag cttcctcaag cacgcgatcc ggcagtcgga
      421 acgctacggc gttcccgcgc atcagctctt gcaccgcgcc ggtgaacgca aactcattgg
      481 tgggcaagaa gatcagctga tcgacatcgc actggagatc aagcgcgagc aggaatcagc
      541 ttctcgcggg ggaacgtaac ggtcgtcagc ggcgcgccga cgctgtcaat ggtcggccat
      601 tcgcgtcttg gagcaggcag atcacatagc tcggggcagg acccgtagtc ggatcgattg
      661 tggtgcggtc ctggttggag tcgaccagaa acgtcactgc gagttcgctg gcatcaaccg
      721 actggcccgt gtacttggga aactgcgcac tgcacgccgg attggcgact tcggggatcg
      781 cggcattgac cgtcaacggt ccgcggaaga acacttcggc catatgcggg gtggcgcagt
      841 cgacgagggc cacggtcacc acgctcgggt cgcttggcgg agggtcggcc aagcagtcac
      901 cgacgtcgag gtcaacccac ttcgcggtcc gagtcgcgga cgtggtgggc ggcgccgcga
      961 cggccgacgt ggttgtcgtg gtcgttgctg gcgtactcgg cgtcgtctcg ccggaactgt
     1021 ttgtggcacc accacatgcc ggcagcaccg caagcgcgct caaaagagtt gtcaggcccc
     1081 acactcgttt cggcagttac tccccctcga tccgtaaacg tcatcggtcg gtcgcaaagt
     1141 ttaggtggta acgcaccggt tctgcttctc gatatagcag ctacctgtcc caaaccagag
     1201 gagcgaaacc cgtgatcatt gccagaggaa acatccggag aggcgtgttg ggcgtcatcg
     1261 ctgccggtgt gctgtccggt gtcgcgaccg cgatcgtcgt actgccgtcc gccaaaggcg
     1321 agcccacgcc cacgcccgcg gccgatcctt gcctcgcgtc gaatctgacc ggaactatca
     1381 gcaaggtcaa tcaagagatc tcggcatacc tgcaatcgca tccggagacg aaccaggcgc
     1441 ttgctgacgt cgccaagcag tcaccgttct cggcgcagtc ggccttcacg gcgtacttcg
     1501 atgcccatcc tgccgaagcg gccgacctgc atacgctgca gcaacctctg gtcgacctca
     1561 gcaaccagtg cggataccag gtgacgccgg gacaggtgct cggtgcgctg agcgacctgt
     1621 agaccagggc cgcgaggtgg gcggcctgcg ggcaagcgag gggtggccag caggccagct
     1681 cagcgacgcg gcgtgacccc gccgagttgt gcggtcagcg cgtcggccgc gaccaccagc
     1741 tcacgggccc gttcggtgat ctccgcgtcg gtgagcgcgc ggccgatctg cagtgacgcg
     1801 accatggctt gcctccgatg gtggtcgtac accggagcgg agatgacgct gacgtcgtgg
     1861 cgctgcccag cttcggagtt ctcgccacgc aggtacacgc gttcgccgat atcggagacg
     1921 atctcgccca gcagcgcgcg cagttcgtcg ggcaggctgt tggacatgcc cgccatcagc
     1981 gcgtagagcc gctgcccgcc gggggtcagc cgctcgacca aatacccgtc ggcgcgacac
     2041 tcggcgatca cgcgatgcag gcgttcggtg tcggtgcgca gcggaatagt gggctccttg
     2101 gccagccagt cgcgaacggc ttcgtcgtcc cacaacacga acatcagccc gaccggcggc
     2161 gcgaacggat agctctggcc cacctcgacg cccactcgcg caccgggcgg cgcgaccagc
     2221 tcgagtagcg cgatccggtc gtcgaccacc gcggaaagtg cagccgtggt ggcgaatcgg
     2281 gtcgacagcc ggcgcagttc ctcgcccgcg gccgggctga cccgcatcga ttcttgggcc
     2341 ctgtggccga gcgtgatcaa ggccgggccc aatcgatatg tcttgtctcg ttcatccctg
     2401 atcaaatagc cggactcggt aagcgtcgtg acgatcccca ggcaggtcgg cttgctgagc
     2461 tccacccgtc gggccaactc ggacaccccg aatcgctcct gcggatgctg cgacaggaag
     2521 tcgaggacgc gcaccgcgcg gtcggtcggc ggcgaggcac ggccggcaga ggcggagagg
     2581 tccatccgca ggatcctacg cgaccgttcc atatatgaac cgcggcggat caatatcttg
     2641 accacatcta gaacacgttc tagtgtcggg ccggtgtaca ccgacccgtt gaccgcagcg
     2701 atcgccgagg ccgaagagct cgtcgcggcc gccccgcata tcgagacgga agcggacctc
     2761 ctcgaaggcc tgcaatatct cgcgcagggc attgccgcgt gcacacatgt gacgttccat
     2821 cacgaccgag accacccgtt cttcctcagc ggcaccggcc ccttcacgaa gatgggcttg
     2881 gacaaccccg acaccctcta tttcggcact cgagtccggc cggaccacga gtacgtcgtg
     2941 tcaggggtgc gtggcaccac caccgacctg agctttcagc tgctcggcgg cgagtacacc
     3001 gacgagggag tgccggtcag tgaagcggcg ttcgacgacc gcgagctcga catcgccgcc
     3061 gacggcagct tcgaatggcg gttcacccca gcgactccgg cgcagctggt cgtgcgggag
     3121 gtctacggcg actggtccca gcggcgcggc acgatcgcga tcaaacgagt cgactcggcg
     3181 ggcaccgcac cgccaccatt gacgcgcgaa acgatcgaaa agcgttacgc cgcagcagga
     3241 aagcagctgg tgaaccgcat caagacgtgg ctgcagttcc cgcagtggtt ctatctgaac
     3301 cttcccgcca acacgttggt cccgccgcgg ctcacgcccg gcggcctggc gacgcagtat
     3361 tcgtcggcgg gccatttcga tctctcgccg gaccaggcgc tagtcatcac cattcccgaa
     3421 tccgacgcgc cctacctagg gttccagctc ggcagcctgt ggtacatctc gctggactac
     3481 atcaaccacc agacgtcgct gaatgcaact caggcgcaag cagatccgga cgggaagatc
     3541 cggatcgtgg tggcggagcg cagtcccggc gtcaccaact gggtcgagac gctgggtcac
     3601 cacaaaggat tcctgcagtt ccgctggcag cgggtgtcac ggaaactcac cgaagccgac
     3661 gggcccactg tcgaattggt cgacttcgac gcggtccctg ccacattacc gttctacgag
     3721 cacaacaaga taaccgacga agattggcgc gagcggattg cacagcggca gcgcgcaatc
     3781 gccacacgga tgctggggtg aacatgctcg aaggcaaagt ggtgtgcatc agcggggtcg
     3841 gcccggcgct ggggacgacg ctggcgcggc gatgcgccga gtacggcgcg gatctggtac
     3901 tggcagcgcg gaccgctgaa cgcctcgacg acgtcgccaa gcagatcacc gacaccggcc
     3961 ggcgcgcggt ggcggtcgcc accgacatca ccgacgacga tcaggtcaag aacctggtcg
     4021 acgcctcgct ggccgcgtat gggcgcgtcg acgtgttgat caacaacgca ttccgagttc
     4081 cgtcgatgaa gccgttggcc aacaccacgt tccaacacat ccgcgacgcg atcgaactga
     4141 ccgtgctggg cgcactgcgc ttaactcaag gtttcacgcc cgcgctggcc gaggccaacg
     4201 gctccgtggt gaacgtgaac tcgatggtga tccggcactc ccaggccaag tacggtgcct
     4261 acaagatggc caagtcggcg ctgcttgcga tgtcgcaatc gttggcgacg gaactcggcg
     4321 agcaggggat ccgggtgaat tcggttgtgc cgggctatat ctggggtgac acgctgaagg
     4381 gctacttcaa ccatcaggct ggcaagtacg gcaccaccgt cgaacagatc taccgcgcga
     4441 cagccgcgaa ctccgacctg aagcgtttgc cgactgaaga cgaagtagcc tcggccatca
     4501 tctttttcgc tggcgatctc tccagtggca tcaccgggca gacgctcgac gtcaactgcg
     4561 gggagtacaa ggcttgatgg cggagcgcac cgacgtcggc accgtcgacg atttgcatga
     4621 gtcggcctcg cgcctgatcg gtctcgacga cttcggtctg gacgacgaca actaccgcga
     4681 aggtcttgcc gtcatcctcg attcgtat
//
LOCUS       DASWBF010000514        29154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_117192,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000514 DASWBF010000000
VERSION     DASWBF010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 29154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29154
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117192"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VF874_18900"
     CDS             complement(<1..1248)
                     /locus_tag="VF874_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9178047.1"
                     /translation="MPRFDLLGPLPAERSTTVLEASAGTGKTFALAGLVTRYIADGAA
                     TLDQMLLITFGRAASQELRERVRRQLVDAVVAFDDPSTVGDNQVLAYLLDGPQRDERK
                     QRLRDALAAFDAATIATTHQFCQLVLKSLGVAGDTDAGVTLAEDLDELVTEIVDDLYL
                     AHFGQERDEPVLTYKDALRLAREVVKNPSTELRPLNPSPGSRAAVCLDFAKGVLAELE
                     TRKRQQGLLSYDDLLIRLADALDGHDSPARVRMHHRWPIVMVDEFQDTDPVQWQVLDR
                     AFSGRSTVILIGDPKQAIYAFRGGDIVTYLQAAETAGDKQTLGTNWRSDGALVDRLQV
                     VLRGAELGDPAIKVHDVEAQHQGHRLSGAPRNDPFRLRVVRRETLGRSGFANLPIDEL
                     REHISADLAADIGALLAGGVTYDG"
     gene            complement(1248..4532)
                     /gene="recC"
                     /locus_tag="VF874_18905"
     CDS             complement(1248..4532)
                     /gene="recC"
                     /locus_tag="VF874_18905"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEX9178048.1"
                     /translation="MPLHLHRAERTDLLADGLGALLSDPLPDPFAEELVIVPAKGVER
                     WLSQRLSHILGRGHGADGVCAGVSFRSPHSLIAEITGTVDDDPWSPDAMVWPALDVID
                     CSLDEPWCRTLATHLGHFEHDEEKELRQGRRYAVARRLAGLFASYARQRPQLLVDWLD
                     AKTDGLDTDLAWQPELWRVLVARIDADPPHIRHQKTVVRLQESPTDLPTRLSLFGHTR
                     LAVTDIELLDALATHHDLHLWLPHPSDDLWEKLADEHRPLPRRDDTSHRNVGHPLLAT
                     LGRDLRELQRNLPHPKTDEYLGGAIKPDTLLGWLQSDITANAVRPEGRVHTKEDRSVQ
                     VHSCHGPARQIDVLREVLLALLADDSTLEPRDILVMCPDIETYAPLIVADFGLGDVIK
                     GVHPAHRLRVRLADRSLVQTNPLLGVASQLLQLAGSRTTASEVLNFAQSAPVRARFGF
                     TDDDLEGVTRWVRQANIRWGFDQEHRKPFGVDFVHNTWRFGIDRVLAGVAMSDDSHAW
                     IDATLPLDDVGSNRVELAGKLAEYVDRLQRTVDQLTGTRPLHQWLTALTDGITLLTRI
                     GDDDTWQTSQMQREFADVLTQAGPRADTLMRLPDIRALLKRHLAGRPTRANFRTGTLT
                     VCTMVPMRSVPHRVVCLVGLDDGVFPRLGVVDGDDVLARNPMTGERDIRSEDRQLLLD
                     AINAATEHLVITYTGANEYSGQPKPPAVPLAELLDTLDITTPQQVRDRVCVEHPLQPF
                     DIRNVERGKLVPGVPFSFDPTVLRAARTAAGQRIEQPQFIAGPLPPPPPDDVVLADLV
                     AFFKDPVKGFFRALDYTLPWEVDGVEDAIPVDINALEEWQVGDRMLGDILGGLTAMQA
                     QQAEWRRGTLPPGQLGWRKAIEIREQAELLAAAARQSRDQRGQAYDVDIDLGSGRRLT
                     GTVSPVFGERLVSVTYSKLDGRHLLESWIPLLALIAHDPDRDWCAVCIGRQRRGTTPC
                     IEGLGWPADDAVEVLRELVSIYDAGRREPLPLPIKTSHAWAEARRCDEDPVREAEYRW
                     KSSDRYPGEDQQPAHVRAWGKNARLADLMQPLRPGEEFDGEGNRLGAYAARLWLPMLR
                     AKRTPE"
     gene            complement(4682..5338)
                     /locus_tag="VF874_18910"
     CDS             complement(4682..5338)
                     /locus_tag="VF874_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178049.1"
                     /translation="MRKVVWWRAAAVALVAASPTAAHAGADPGATEVITVAAVGTNGE
                     PLGGYHVVDRQTSANLSGCPGPSPAATAPNVYACEPSQAAAEVCWPAPASVLCTVDPW
                     SKTLRRFSSPGALPAVNPPATPMPFALLLDDGTRCVLPNGVDWGGRADGTVPIYGCSL
                     TSPSLGVLAPPDWNPVDAIDRTQPLWAVFVGQLGPRDTPFQPPIRHTLTTAWFAGNAG
                     "
     gene            5631..5915
                     /locus_tag="VF874_18915"
     CDS             5631..5915
                     /locus_tag="VF874_18915"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9178050.1"
                     /translation="MGLNIKNERITALVRQLAQRTGMTQTGAIEDAVRSRLAELNKEQ
                     ADGDGSGGSEVKEANARQLLDELRRSLTATERSELKAAEGNLYDDTGLPA"
     gene            5912..6310
                     /locus_tag="VF874_18920"
     CDS             5912..6310
                     /locus_tag="VF874_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9178051.1"
                     /translation="MIVDTSAIVAVVLDETHADPIVEILSSTTDLKMSAATAVELNAV
                     LTRRLRPEDQRRVERLLDVWEIEVVSFDAAQAGIASRAYRDFGRGSGHPAALNLGDCY
                     SYALAVVTGEPLLFVGDDFVHTDVAAAYRP"
     gene            6361..7497
                     /locus_tag="VF874_18925"
     CDS             6361..7497
                     /locus_tag="VF874_18925"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX9178052.1"
                     /translation="MRTSIKRIALSTGGGDAPGLNAVIRAATLAARNRDWDVVGIRDG
                     FNGLLSPDDYPDGGLIELTRDRVRGIVYQGGTILGTTNRGNPIAYPVQQDDGSWVEVD
                     RTDELLGRFEEHRIDALITIGGDGSLSIARHLNEAGLHLVGVPKTIDNDLENTTSTFG
                     FETAVEFASQCIDRLFSTATSHGRIIVVEVMGRYAGWIALHAGVASGVHAILIPEIPF
                     SVEPVAALITHRERHGAKFSIVCVAEGARPVGGELSIVGKSVGQAERLGGIGAKVAAE
                     LEELTGRETRTVVLGHLLRGGSPTSQDRLLGMRFGAAAVRALDEGHNGVMVALNPPTV
                     DLVPLAEATRRQRKVPLDCDTILTARDMGINFGDEMPTRSAIGR"
     gene            7500..8665
                     /locus_tag="VF874_18930"
                     /pseudo
     CDS             7500..8665
                     /locus_tag="VF874_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            8707..9129
                     /locus_tag="VF874_18935"
     CDS             8707..9129
                     /locus_tag="VF874_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9178053.1"
                     /translation="MNDIPSMGSTPVYTITGDPVARVAADATVADVAKALVAGEVGAV
                     VVGDDERPAALASERDVVRVVAAGKDPASVRALEVASTKLVWCEADATIDEVAVQMMD
                     HYIRHILVDRDGALVGIVSARDLLGVYCAEADLGRGSA"
     gene            9126..10655
                     /locus_tag="VF874_18940"
     CDS             9126..10655
                     /locus_tag="VF874_18940"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX9178054.1"
                     /translation="MKLRSAAAAIGGGLAGLAGWDLLQRRHAILRNYPIVGHLRFILE
                     GVGPELRQYIVTDNDAEKPFSRDQRRWVYTSSKLRNRYFGFGTDNDLERVHNYPIIKH
                     AAFPLSTPDGEPTHPDPVVPLPAAKVLGGACKRTKAFRPSSLVNVSAMSFGSLSGAAI
                     TALNKGAAMAGALHTTGEGGVSPHHLNGGDLVWQIGTGYFGCRNDDGTFSLSRLIDQV
                     AATPSIRAIEFKLSQGAKPGLGGMLPGVKVTPEIAAMRGVPVGVDCRSPAGHSAFRDV
                     DGLLELVETVAAATGLPVGVKSAVGEATFWVDLASRMARTGRGVDFVTVDGGEGGTGA
                     APLVFSDHVALPFKWAFPRVYRAFAEQDLHHEVVFVGSGKLGIPENALLAIAMGCDMV
                     NVARTAMFSIGCIQAQRCHTGRCPTGVATQSPWLERGLDPALKSVRCANYLATVRFEL
                     LSLARACGQVHPALVPLDAIELLDVDLRTVRVDELFDYEPGWGMPGPADVSAITGLMG
                     G"
     gene            complement(10756..11745)
                     /locus_tag="VF874_18945"
     CDS             complement(10756..11745)
                     /locus_tag="VF874_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9178055.1"
                     /translation="MKGYDIIGDVHGCATKLEQLLDDLGYAPDETGAYRHPERRAVFV
                     GDLIDRGPEQLRVLEIVKPMVDSGSAQIVMGNHEFNAIAYHTEHPERSGEYLRPHNEK
                     NQAQHQKFLDQVTGEQRAYYLEWFKTLPLWLDLGDLRIVHACWHEKSMKVVEQELGSN
                     RLNTLDQLVRASTKGERLYDAVEILLKGPEISLVDRGQPPYLDKDGHRRTEARVRWWK
                     EGATTLHALAEMGGKLRTEDGAPYPELPGIEVPADERSYVYTDDVPVFYGHYWREGTP
                     EHLLDWTAHSACVDFSAVKGGTLVAYRWSGESRIRLDHYVPRTEDVVAQHPSA"
     gene            12409..12912
                     /locus_tag="VF874_18950"
     CDS             12409..12912
                     /locus_tag="VF874_18950"
                     /inference="COORDINATES: protein motif:HMM:NF025407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppP/LprE family lipoprotein"
                     /protein_id="HEX9178056.1"
                     /translation="MSRVLVGSAAVCAFAGLIGGIPAASADPFPSCGPDQATAIGSAT
                     ARDRHDTITQAPWNPVPVQSNYDACADLSAAVITIDMARPTSPRQVFLFHRGTYIGNA
                     TNNSRPFTTLDAAASTPNTVVLDFVSGRTCGTCDDGQTYPVRYYWNGVTAVMPDPIPP
                     RQDWPIP"
     gene            12923..14002
                     /gene="galT"
                     /locus_tag="VF874_18955"
     CDS             12923..14002
                     /gene="galT"
                     /locus_tag="VF874_18955"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX9178057.1"
                     /translation="MTAPTTWRLADGRDLLFFSLPGHTPAPVTDRRPLPERDRHQSQL
                     RLDRQTGQWVTVAPRRQNRTYKPGADSCPLCPDPSGLTTEVPAADYDVVVFENRFPGF
                     AGAADGTPVMTDDLFASVPGHGHCEMICFCSNHLGSFADLDHSHARLVVDAWRHRTAD
                     LMTRPGIEQVFCFENRGEEIGVTLTHPHGQIYGYPFLTPRTAEMLRQAQAYRSASGRN
                     LFGDLLAQELSDGSRVVTHTELFTAFVPFAARWPVEVHIYPNRFVHNLVDLDDAELDE
                     FAQVYLDILNRFDRLYSIPLPYISALHQYADDDVQRDGYFHVELMSIRRTATKLKYLA
                     ASESAMGAFISDVTPESIAARLREV"
     gene            14004..15059
                     /locus_tag="VF874_18960"
     CDS             14004..15059
                     /locus_tag="VF874_18960"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX9178058.1"
                     /translation="MITYGAPGRINLIGEHTDYNLGFALPIALPERAVVTYSPSDSDA
                     LNVRSDAEPGEVRIPLDTAPGDVTGWAAYVAGVVWSLRRAGHRAVGGDMSIESGVPIG
                     AGLASSAALECAVLGAILEATGARVDRLEQARIAQAAENDYVGAPTGLLDQLACLFAE
                     PGAAVLIDFRAVAVQLVPFDPDASDVALLLVDSHARHRHAGGEYAARRASCESEAADL
                     GVASLRDVQVPPRNRYARHVVTENQRVLDFVAALSDCNFGRAGHLLTASHASMRDDFD
                     ITTDHIDLIAATALQAGALGTRMTGGGFGGCVIALVPTDEIDRVGDAVRRAIRDAGYP
                     EPTVRHVRAGRGASAEA"
     gene            15081..15857
                     /locus_tag="VF874_18965"
     CDS             15081..15857
                     /locus_tag="VF874_18965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9178059.1"
                     /translation="MATSNLSRSVADRTAFITGAASGIGRAAAHVFAAEGVRLALADI
                     QSQAVEQVAAAVGASGGQAQAWTLDVADAAAVASTVPRAADALGGLDFVINNAGVVRP
                     TNLDDEGYDELWHQQLDIMLTAHQRIVRAALPYLRRSSCPRIVNVASTEAMGATRGNG
                     IYAVAKHGVVGLTRALAVDLGREGITVNCVCPGPINTALTEPIPDEHKIIFAKRRTAL
                     GRYGEPAEVAHLILSMCLPAASYVTGAVIPVDGGLMARNA"
     gene            complement(15892..16581)
                     /locus_tag="VF874_18970"
     CDS             complement(15892..16581)
                     /locus_tag="VF874_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9178060.1"
                     /translation="MGERVHFSVDESVATIAMDDGKVNALSPDMQLEINEALDHAELE
                     GVGAVVISGNNRVFSGGFDLKVLTGGGDAAIEMLAGGFELAARILAYPKPVVMACTGH
                     SIAMGSFLMLCGDHLIGAPSHRVQANEVSIGLTMPGPALAILRHRLTPAAFQRAVGLS
                     AEFKGESAVAAGYLDELVEADMVRPRAHEVAKGFTSLDARAHKESKLRARARVLDEIR
                     TTIAAEFRPPR"
     gene            complement(16597..17313)
                     /locus_tag="VF874_18975"
     CDS             complement(16597..17313)
                     /locus_tag="VF874_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9178061.1"
                     /translation="MATDDRLYFRQLLSGRDFAQSDMIARQMRNFAYLIGDRETKDCV
                     VVDPAYAATNLVDALEQDGMHLSGVLVTHHHPDHVGGTMMGFQLKGLAELLDRTEVPV
                     HVNTHEALWVSRVTGIPLNDLTKHEHGDKVSIGDIEIELLHTPGHTPGSQCFLLDGRL
                     VAGDTLFLEGCGRTDFPGGDVDEMYRSLHQLAQLPGDPTIFPGHWYSEEPSASLSEVR
                     RTNYVFRPTSLEQWRMLMGA"
     gene            complement(17549..18409)
                     /locus_tag="VF874_18980"
     CDS             complement(17549..18409)
                     /locus_tag="VF874_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9178062.1"
                     /translation="MTEGATRVAVYMDFDNIVISRYDQVHGRNSFQKDKSKGLDAESL
                     TRATVDVGAIIDFASSFGTLVLTRAYADWSADLNAGYSGQLVARAVDLVQLFPAAAYG
                     KNGADIRLAVDAVEDMFRLPDLTHVVIVGGDSDYIALAQRCKRLGRYVVGIGVAGASS
                     GSLAAACDEFVTYDALPGVPSLPKDAPKKRSRRAATQEPDDETPSQDVQLAATNLLER
                     ALRMGLQKDDTEWLHNSAVKAQMKRMDPSFSEKSLGFRSFSDFLRSRSEIVDLDESTT
                     TRMVKLHSET"
     gene            18670..18981
                     /locus_tag="VF874_18985"
     CDS             18670..18981
                     /locus_tag="VF874_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178063.1"
                     /translation="MTTRRCLRGTELRYVLTHHLFLHGSATVVELIDMLDWHGFETRG
                     RPSKAISDALRWEMGRGRVCRRGRGLYVPVWMPRGTEHRIHARGLALRAEAERWRRDS
                     A"
     gene            19055..19127
                     /locus_tag="VF874_18990"
     tRNA            19055..19127
                     /locus_tag="VF874_18990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19088..19090,aa:Thr,seq:ggt)
     gene            19141..19214
                     /locus_tag="VF874_18995"
     tRNA            19141..19214
                     /locus_tag="VF874_18995"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19175..19177,aa:Met,seq:cat)
     gene            19247..19414
                     /gene="rpmG"
                     /locus_tag="VF874_19000"
     CDS             19247..19414
                     /gene="rpmG"
                     /locus_tag="VF874_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX9178064.1"
                     /translation="MASSTDVRPKITLACEVCKHRNYITKKNRRNDPDRLELKKFCPN
                     CGKHQAHKETR"
     gene            19465..19944
                     /gene="hadA"
                     /locus_tag="VF874_19005"
     CDS             19465..19944
                     /gene="hadA"
                     /locus_tag="VF874_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(3R)-hydroxyacyl-ACP dehydratase subunit HadA"
                     /protein_id="HEX9178065.1"
                     /translation="MSLSEKIVGMHYRYPDYYVVGREKVREYATAVKNDDPAFFEEKA
                     AAELGYDGLLAPLTFISVFGYQAQSAFFASANVTVKDAKIVQVDQVLKFMRPITVGDR
                     LYCDVYVDSVRQSFGTDTIVTKNIVTNEDGDIVQETYTTLVGRSEGEGEEGFSDAIA"
     gene            19931..20359
                     /gene="hadB"
                     /locus_tag="VF874_19010"
     CDS             19931..20359
                     /gene="hadB"
                     /locus_tag="VF874_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(3R)-hydroxyacyl-ACP dehydratase subunit HadB"
                     /protein_id="HEX9178066.1"
                     /translation="MPLREFDSVKVGDQLPEKVITLTRQDLVNYAGVSGDLNPIHWDD
                     DIAQQVGLDTAIAHGMLTMGLGGGYVTSWIGDPGAVTEYNVRFTAVVPVPNDGRGADI
                     VFSGRVKSADPETKLVTIAVAATSGGKKIFGRAVATAKLA"
     gene            20364..20873
                     /gene="hadC"
                     /locus_tag="VF874_19015"
     CDS             20364..20873
                     /gene="hadC"
                     /locus_tag="VF874_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(3R)-hydroxyacyl-ACP dehydratase subunit HadC"
                     /protein_id="HEX9178067.1"
                     /translation="MALKTDIRGKIFKYPDFFEVSREQIRQYARAVKAEDPATFDEDE
                     AHALGYDTIVAPLTFSAIFALLIQQHLFRQVDIGMETMQIVQVDQKFLYHKPIQAGDR
                     LFGTVYVHSVDERFGADIVVTRNVCENENGEVVMEAFTTLMGQQGDNSTQIKWDAESG
                     QVMRKAVGE"
     gene            21031..21103
                     /locus_tag="VF874_19020"
     tRNA            21031..21103
                     /locus_tag="VF874_19020"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21064..21066,aa:Trp,seq:cca)
     gene            21176..21619
                     /gene="secE"
                     /locus_tag="VF874_19025"
     CDS             21176..21619
                     /gene="secE"
                     /locus_tag="VF874_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX9178068.1"
                     /translation="MSDEREAAGAAGDGEDTTTSGGRTAVATRPLRPTGKRSRQVTEP
                     VEAEPIEKAEKTKDRGKKKAKTAKKTGPDKGRPNPIAFVVNYLKQVVAELRKVIWPNR
                     KQMVNYTAVVLVFLVFMVALIFVVDVGLAALVRGVFAGEWALRFR"
     gene            21689..22387
                     /gene="nusG"
                     /locus_tag="VF874_19030"
     CDS             21689..22387
                     /gene="nusG"
                     /locus_tag="VF874_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX9178069.1"
                     /translation="MPAAQAVDVIDDEAADVAGEATPEEELDPAEALKADLRTRPGEW
                     YVVHSYAGYENKVKANLETRVQNLDVGDYIFQVEVPTEEVTEIKNGQRKQVNRKVLPG
                     YILVRMDLTDDSWSAVRNTPGVTGFVGATSRPSPLSLDDVVKFLLPQGAAKKTTKAAA
                     AGAAAETGGIERAPIEVDFEVGESVTVMDGPFATLPASISEVNAEQQKLKVLVSIFGR
                     ETPVELTFTQVAKI"
     gene            22438..22866
                     /gene="rplK"
                     /locus_tag="VF874_19035"
     CDS             22438..22866
                     /gene="rplK"
                     /locus_tag="VF874_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX9178070.1"
                     /translation="MAPRKKVSGLIKLQIQAGQANPAPPVGPALGQHGVNIMEFCKAY
                     NAATENQRGNVVPVEITVYEDRSFTFNLKTPPAARLLLKAARVDKGSAEPHRVKVAKV
                     SWDQVREIAETKKDDLNANDIDAAAKIIAGTARSMGITVE"
     gene            22967..23674
                     /gene="rplA"
                     /locus_tag="VF874_19040"
     CDS             22967..23674
                     /gene="rplA"
                     /locus_tag="VF874_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX9178071.1"
                     /translation="MSKKSKAYQAAAEKVDRSQLYAPMAAVKLAKETSSKNQDSTVEV
                     AIRLGVDPRKADQMVRGTVNLPNGTGKTARVAVFAVGDKASEAEAAGADIVGSDDLLE
                     RIQGGFLDFDAAIATPDQMAKVGRVARILGPRGLMPNPKTGTVTPDIAKAVADIKGGK
                     INFRVDKQANLHLVIGKASFDENKLVENYGAAIDEVLRAKPTASKGRYLKKITVSTTT
                     GPGIPVDPSVTRNFAEA"
     gene            complement(23717..23848)
                     /locus_tag="VF874_19045"
     CDS             complement(23717..23848)
                     /locus_tag="VF874_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178072.1"
                     /translation="MLWTNRFTERAIRYQHRAERDPDPHQEDQAERQHMTEYSELAH"
     gene            23870..24505
                     /locus_tag="VF874_19050"
     CDS             23870..24505
                     /locus_tag="VF874_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEX9178073.1"
                     /translation="MTVVGLVFGALLGFTVVVSWEQFSSAEANVAHEASTLTTMYRQT
                     VGMPDPEQTKLREQLRKYAEAAKGPDWEALDSSGKGDGARSAITQMYRLLGNQPDASS
                     NPVNGEFLSQLTVLANDRNQRVLDAQPRIPGLLWAGLLFGGIVLVALTGFMRLDNRLG
                     HAILSSAIAVLLGLLLYIIYWLDHPLGTDIGVTPVPFRHSLEVFDIIDRGT"
     gene            complement(24506..25564)
                     /locus_tag="VF874_19055"
     CDS             complement(24506..25564)
                     /locus_tag="VF874_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX9178074.1"
                     /translation="MPEGRLANPDCTLGTDPRSDPRMIAALAPFGLDGLLPLPPVTPD
                     SPLPDRVAYVTEAEEVLVDVLDALARIAPVAEAVTNETTTIRGDDGNDITLYISRPSG
                     TTAVAPCIFHIHGGGMAFGSATDIGYVRWRDNLAATGLVVVGVEFRNSAGELGPHPYP
                     AGLNDCAAGLRWTAAHLGDLGASHIVVSGESGGGNLTLAVAHKAKREAWLHEIAGVYA
                     QCPYISNRYLEQPDDLPSLKENDGYIFNSMEMAVIGSVYDPDGEHSGDPTCWPGNATE
                     ADLAGMPPHVISVNELDPMRDEGLAYYRALLRAGVPTLGRIIAGTCHAGDMLCAGALP
                     DVYGATVRDISGFAKSLA"
     gene            complement(25574..26803)
                     /locus_tag="VF874_19060"
     CDS             complement(25574..26803)
                     /locus_tag="VF874_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9178075.1"
                     /translation="MRDLDGVFRREWGPAVAALARWSGDLTVAEDAVQEAFTEAARAW
                     PRDGVPDNPGAWVVTVARNRARDRLRRESVRPGKELAAVVDDIRARTDTADPHPVRDD
                     ELRMMFTCAHPALDRTSQLALTLRLVSGLTVPEIARALLQTEAAIGQRITRAKSKIRH
                     ARIPLKVPSAERLPERTPHVLGCIYSVFTEGYWSTAGPSAIRDELCTEGVRLAGELAG
                     LMPDEPETQALSALVTLHDARRLTRVDGDGALVPLDEQDRTRWDAMAIARGLRRVRRA
                     AGSTGPYLPQAVIAALHATAPTFEQTDWGGVCAAYDRLLAVNDSPVVRANRAVALGFR
                     DGFAVGLKALDEVAGDPRLVRSSLVRSVRADLLRRDGRADEALPWYQEALAVTGSEPA
                     KAFLRRRIAECGGRGDA"
     gene            complement(26807..27517)
                     /locus_tag="VF874_19065"
     CDS             complement(26807..27517)
                     /locus_tag="VF874_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9178076.1"
                     /translation="MQYFALLVAPSDAPEERADAAALEAEMAAYNDFHARERSSIKAG
                     DALTPAPAGVRITGGRDAPVVTDGPFPESAEIAGGFYVFEADDLDAALALTRQIPHAR
                     KGAVEVWPMAMEFTPTTKLTDEHWLALLLEPPATVSAPGTPEWEAGAAKHGAFGAAAG
                     PHIIGGAALHEPSTVTTVRLRDDEVLLTDGPFVEGTEVANGFYVLSAADRDEAVKLAS
                     MIPATTVELRQLMGIAGL"
     gene            27813..>29154
                     /locus_tag="VF874_19070"
     CDS             27813..>29154
                     /locus_tag="VF874_19070"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HEX9178077.1"
                     /translation="MPGTGTPNANIVANYKENARDRYMTTTPCTVANGCTGANLIGIN
                     YPASFWPLGFPPFPSTWCPGLKCDTWNVSAGEGLAHLKSALITVLSDPTNPDIVIFGY
                     SQGGAVVSAEMYNLASLPQTTKDRIKVVTIGNISNPQGLWARLSFLPTIQILNVTFGP
                     LLPTDIGIESTNYVFEYDPVGDAPLNWWNPLALLNAVFALQYVHGNYLLPNGNDPTGT
                     LPYGYTPETLAAAIQDPNNIRTYQDATFVLIPQKGTLPLFQPIADLAAATGTTPFVKP
                     ILDLISPVFKVLIDLGYDRTLNPGIPRTLNLIPIFNPIKLAVDLVVAAGEGIQAAWKD
                     ITGTAPALPAPFAPSGPSILAADNAAKLARTTEIEGAKPESQSPLKINPRDGLSPLKI
                     DPLKIDPKPDADAGIVAPKTYPSQIGFDESLNAAFSLPKTPKPGLSAVPPLKLPK"
BASE COUNT         4931 a         9674 c         9703 g         4846 t
ORIGIN      
        1 tccgtcgtag gtcaccccgc cggcgagcag cgcaccgatg tcggcggcga ggtcggcgct
       61 gatgtgttcg cgtagttcgt cgatcggtag gttcgcaaag ccgctgcggc ccagtgtttc
      121 tcgtcgcacc acccgcagcc ggaacgggtc gttgcgcggc gcgccggaca gccggtggcc
      181 ctggtgttga gcttcgacgt cgtgcacttt gatcgccggg tcgccgagtt cggcgccgcg
      241 cagcaccacc tgcagccggt cgacgagcgc gccgtcgctg cgccagttcg tgccgagtgt
      301 ctgcttgtca cccgctgtct cagcggcttg cagatacgtg acgatgtcgc cgccgcggaa
      361 cgcgtagatc gcctgcttcg gatcgccgat caggatcacc gtggaccgcc cgctgaatgc
      421 gcggtcgagc acctgccact gcaccgggtc ggtgtcctgg aactcgtcga ccatgacgat
      481 cggccatcgg tgatgcatcc gcacgcgggc gggggagtca tggccatcca gcgcgtccgc
      541 caaccggatg agcaggtcgt cgtagctgag gagtccctgt tgccgcttac gcgtttccag
      601 ctcggcgaga acgcctttcg cgaagtcgag gcacaccgcc gcgcgcgagc cagggctggg
      661 gttcagcgga cgcaactccg tcgacgggtt cttgaccacc tcacgggcga ggcgcagcgc
      721 gtctttgtag gtcaacaccg gctcgtcgcg ttcctgcccg aaatgcgcga gataaaggtc
      781 gtcgacgatc tcggtgacca gctcgtcgag atcctcggcc agcgtgacgc ccgcgtcggt
      841 gtcacccgcc acgcccagcg acttgagcac cagctggcag aactggtgcg tggtcgcgat
      901 ggtcgccgcg tcgaaagcgg ccagcgcatc gcggagtcgc tgtttgcgtt cgtcgcgctg
      961 agggccgtcg agaaggtatg ccaggacctg gttgtcaccg acggtcgacg gatcgtcgaa
     1021 agcgacgacg gcgtcgacca attggcgacg gacccgctcg cgcagctctt ggctggccgc
     1081 ccgaccgaat gtgatcagca gcatctggtc cagcgtcgcc gcaccgtcgg cgatgtagcg
     1141 cgtgaccaga cccgccagcg cgaacgtctt cccggtgccg gcgctggctt ccagcaccgt
     1201 ggtggatcgt tcggcgggca acgggcccag caggtcgaag cgcggcatca ttccggcgtc
     1261 ctcttggcgc gcagcatcgg cagccagagt cgggcagcgt acgcgccgag gcgattgccc
     1321 tcgccgtcga actcctcgcc ggggcgcagc ggttgcatca gatccgccag ccgcgcgttc
     1381 tttccccacg cacgcacgtg cgccggctgc tgatcctcac cggggtacct gtcactcgac
     1441 ttccaccggt attccgcctc gcgcaccgga tcctcgtcgc agcgccgcgc ctcggcccac
     1501 gcgtgggacg tcttgatggg caaaggaagt ggctcacgtc ggcctgcgtc gtaaatcgag
     1561 accagttcgc gcaatacctc gaccgcgtcg tcggccggcc acccgagccc ctcgatgcac
     1621 ggcgtggtgc cgcgtcgctg cctgccgatg cagaccgcgc accagtcgcg gtctggatca
     1681 tgagcgatca acgccaacag aggaatccac gattcgagca gatgccggcc gtcgagcttt
     1741 gagtatgtca ccgacacgag ccgctcgccg aataccggtg agacggttcc cgtcagccgc
     1801 cgtccagagc cgaggtcgat gtcgacgtca taggcctgcc cacgctgatc gcgggactgg
     1861 cgggctgcgg cagcaagcag ctctgcctgc tcgcggatct caattgcctt gcgccagccc
     1921 agctgtcccg gcgggagcgt cccgcggcgc cattctgcct gctgtgcctg cattgccgtc
     1981 aggccgccca ggatgtcgcc cagcatccgg tcaccgacct gccactcttc gagcgcgttg
     2041 atgtcgacgg ggatcgcgtc ctccacaccg tcgacctccc acggcagcgt gtagtcgagc
     2101 gcgcggaaga aacccttcac cggatccttg aagaatgcga cgaggtcggc gagcacgacg
     2161 tcgtcgggcg gtggcggagg cagcggcccg gcgatgaact ggggctgctc gatgcgctgc
     2221 cccgcggcgg tgcgagccgc ccgcagcacc gtcgggtcga agctgaacgg cacaccgggc
     2281 accagctttc cccgctcgac gttgcgaatg tcgaagggct gcaagggatg ttcgacgcaa
     2341 acccggtcgc ggacctgctg cggcgtggtg atgtcgaggg tgtcgagcag ctcggcgagc
     2401 ggcaccgcgg gcggcttggg ctgacccgag tattcgttgg cgcccgtgta ggtgatcacc
     2461 aggtgctcgg tggccgcatt gatcgcgtcg agcagcaatt gcctgtcctc ggaacggatg
     2521 tcgcgctcgc cggtcatcgg gttacgggcg agcacgtcgt cgccgtcgac cacgccgagc
     2581 cgcgggaaca caccgtcgtc cagaccgacg aggcacacca cccggtgcgg caccgagcgc
     2641 atcggcacca tcgtgcagac cgtgagtgtg ccggtgcgga agttggcgcg ggtcggccgc
     2701 ccggcgaggt gccgcttcag cagcgcgcgg atgtccggca gccgcatcag cgtgtcggca
     2761 cgtggtccgg cttgcgtgag gacgtcggca aactcgcgct gcatctggct ggtctgccag
     2821 gtgtcgtcat cgccgatgcg ggtgagcagc gtgatgccgt cggtcagcgc ggtcaaccac
     2881 tggtgcagcg ggcgtgtgcc ggtgagctgg tcgacggtcc gttgtagccg gtcgacgtat
     2941 tcggcgagct tcccggcgag ctcgacgcgg ttgctgccga cgtcgtcgag gggcagtgtg
     3001 gcgtcgatcc aggcgtgcga gtcgtcggac atcgcgacgc ctgccagcac gcggtcgatg
     3061 ccgaatcgcc aagtgttgtg gacgaagtcg acgccgaagg gtttgcggtg ctcctggtcg
     3121 aagccccagc ggatgttcgc ctggcggacc catcgggtga cgccctcgag gtcgtcgtcg
     3181 gtgaagccga agcgggcgcg taccggagcg gactgggcga agttgagcac ctcgctcgcg
     3241 gtggtccggc tgccggccaa ctgcagcagt tgcgaggcga ccccgagcag cgggttggtc
     3301 tggacgaggg agcggtcggc gagccggacc cgtaagcggt gcgccggatg gacgcccttg
     3361 atcacgtcgc cgaggccgaa atccgcgacg atcagcggcg cgtacgtctc gatgtcgggg
     3421 cacatgacga ggatgtcgcg cggttccagc gtcgagtcgt cggcgagcag agcgagcagg
     3481 acttccctga gcacatcgat ctgccgggcc gggccgtggc agctgtgtac ttggacggag
     3541 cggtcttcct tggtatgcac gcggccctct ggccggacgg cgttggcggt gatgtcggac
     3601 tgcagccagc cgagcagcgt gtcgggtttg attgcgccgc cgaggtattc gtcggttttg
     3661 gggtgcggga ggttgcgttg cagttcgcgg aggtcacggc cgagggtggc gagcagcgga
     3721 tggccgacgt tgcggtggct ggtgtcgtcg cggcggggga gcgggcggtg ctcgtcggcg
     3781 agcttttccc agaggtcgtc gctgggatgc ggcagccaca ggtgcagatc gtggtgggtg
     3841 gccagcgcgt cgagcagctc gatatcggtg acggccagcc gggtgtggcc gaacagcgac
     3901 aggcgggtgg gcagatcggt cggtgattcc tgaagccgca caacggtttt ctgatgtcgg
     3961 atatgtggcg ggtcggcatc gatgcgggcg acgagcacgc gccacagttc gggctgccaa
     4021 gccaggtcgg tgtcgaggcc gtcggtcttg gcgtcgagcc agtcgacgag cagctgcggt
     4081 cgctggcggg catacgaggc gaacaggccg gcgagccgtc gcgccaccgc gtagcggcgt
     4141 ccctgccgca actccttctc ctcgtcgtgc tcgaagtgcc cgaggtgggt ggcgagcgtg
     4201 cggcaccacg gttcgtcgag gctgcagtcg atgacgtcca gcgccggcca caccatggcg
     4261 tcgggcgacc acggatcgtc gtcgacggtg ccggtgatct cggcgatcag cgagtgtggg
     4321 ctgcggaacg acacccccgc acagacaccg tcggcaccgt ggccgcgccc gaggatgtgg
     4381 gagagccgct ggctcagcca ccgctcgacg cccttcgccg gcacgatgac gagctcctcc
     4441 gcgaacgggt cgggcagcgg gtcggacagc agcgcgccga ggccgtcagc aaggagatcc
     4501 gtgcgctcgg cacggtggag atgaagcggc atttgtcgtc acgataggag gttggtccga
     4561 caaggtcacc agccgctcag cgttcactct gcgctcaggg cgcaagagtg cgagtaggcc
     4621 acgccctcag cgcagagtga ggttgggcac actcaacgac ttgacggcgg acgatgacga
     4681 gtcaaccggc gttgcccgcg aaccacgcgg tcgtcagcgt gtggcgtatc gggggctgga
     4741 acggggtgtc gcgcggaccc agttggccga cgaacaccgc ccacagcggc tgcgtccggt
     4801 cgatcgcatc gaccggattc cagtcgggag gagccaggac gccgagcgac ggggacgtca
     4861 ggctgcatcc gtagatcggg accgtgccgt cggcccggcc gccccagtcg acgccgttgg
     4921 gcagcacgca gcgggtaccg tcgtcgagaa gcaacgcgaa cggcatcggc gtcgcgggcg
     4981 gattgaccgc gggaagcgcg ccgggggagg agaaccggcg aagcgtcttg ctccacggat
     5041 ccaccgtgca caacaccgag gcgggggccg gccagcacac ctcggcggcg gcttggcttg
     5101 gctcgcaggc gtagacgtta ggcgcggtcg ccgccgggga cgggccaggg cagccggaaa
     5161 gattggccga cgtctgccgg tcgaccacgt gatagccgcc gagcggctcg ccgttcgtgc
     5221 cgaccgccgc gaccgtgatg acctccgtcg ctcccgggtc agcaccggca tgcgcggccg
     5281 tcggcgacgc ggcaacgagc gccaccgcag ccgctcgcca ccagacgact tttcgcattg
     5341 acagcctcct tcgagatcaa gcctgccgca tggttggcga tcggcgaggc ggtttcccgt
     5401 tcacgcacgc caggcggtgg ctaggctctc cggggccgcc tctgcgcgac aacgcgttga
     5461 cttgcggtgc aaggcccggt gttgtcgctg atcgatctac tctcgttatg gccgtgacgt
     5521 gcgtagacgc tcttgtcgcg cagaggcggg caagtcgaac tatccggccg gcctcggcca
     5581 taagccgccg ttcggtagat aatctaccgt cgtgggatac cctttctacc atgggtctca
     5641 acatcaagaa cgagcgcatc acggctttgg tgcgccagct tgcgcagcga actggcatga
     5701 cccagaccgg tgccatcgag gatgccgtcc ggtcgaggtt ggccgaactg aacaaggaac
     5761 aggcggacgg cgacggctcg ggcggcagcg aggtgaagga ggcgaatgcg cggcaacttc
     5821 tcgacgagct gcgccgctcg ctcaccgcca cggaacggtc tgaactgaaa gcggccgagg
     5881 gcaacctcta tgacgacacc ggcctgcccg cgtgatcgtc gatacctcag cgatcgtcgc
     5941 agtcgtgctc gatgaaacgc acgccgaccc gattgtcgag atcctctcgt cgacaaccga
     6001 tctcaagatg tcggcggcca cagcggtgga actcaatgcc gttctcactc gtcggctccg
     6061 ccccgaagac cagcggcgcg tcgagcgatt gctcgacgtg tgggagatcg aggtggtgtc
     6121 cttcgacgcc gctcaagccg gaatcgccag tcgggcgtat cgcgacttcg ggcgaggcag
     6181 cggtcacccc gcggcactga acctcggcga ctgctactcc tatgccctgg ctgtcgtgac
     6241 gggcgaaccg ttgctctttg tgggcgacga tttcgtccac accgacgtcg ccgccgcata
     6301 tcggccctag ggcgaaaggt gcccgaacca cgaacaaagc tcgctgggta gggtcggctc
     6361 gtgaggacgt cgatcaagcg catcgcgctg agcaccggcg gcggtgacgc gccgggcctc
     6421 aacgccgtga tccgcgcggc cactttggcc gcacgaaacc gcgactggga cgtcgtcggg
     6481 attcgcgacg ggttcaacgg gttgctgtca ccggacgact accccgacgg cgggctgatc
     6541 gagctgacgc gtgacagagt tcgcggcatc gtctaccagg gcggcacaat cctcggcacc
     6601 accaaccggg gtaacccgat cgcctatccg gtgcagcagg acgacggcag ctgggtcgaa
     6661 gtggatcgga ccgacgagct gctcggccgc ttcgaagagc accgaatcga cgcgttgatc
     6721 acgatcggcg gcgacggatc gctgtcgata gcccgtcacc tgaacgaggc tgggctgcat
     6781 ctggtcggcg tacccaagac gatcgacaac gatctggaga acacgacgtc gacgttcggc
     6841 ttcgagactg ccgtcgaatt cgcctcgcag tgcatcgacc ggctgttctc caccgcgacg
     6901 tcgcacgggc gcatcatcgt ggtggaggtg atgggccgct acgccggatg gatcgccctg
     6961 cacgccggcg tggcgtcggg ggtgcacgcg attctcatcc ccgagatccc gttcagcgtc
     7021 gagccggtgg cagccctgat cactcaccgc gagcggcacg gagcgaagtt ctccatcgtc
     7081 tgtgtcgccg aaggcgcccg gccggtcggc ggagagctgt cgatcgtggg caagtcggtg
     7141 gggcaggccg aacgccttgg tggcatcggc gccaaggtgg ccgccgagct cgaggagctg
     7201 accggtcgag agacacgcac cgtcgtgctc ggccacctgc tgcgcggcgg atcgccgacc
     7261 tcgcaagacc ggttactcgg catgcgcttc ggggccgccg cggtgcgggc cctcgacgaa
     7321 gggcacaacg gagtgatggt cgctctcaat ccgccgacgg ttgacctcgt gccgctcgcc
     7381 gaggccaccc gccggcagag gaaggttccg ctcgactgcg acacgatact gacggcccgc
     7441 gatatgggga tcaacttcgg cgacgagatg cccacgcgca gcgcgatcgg acgctgaccg
     7501 tgaaggccgt cacctgcacc gacgcgaagc tcgaggtcgt cgaccaaccg acgccgacgc
     7561 cggccaaagg ccagttgctg atcgacgtcc ttcgctgtgg catctgcggc tcggacctgc
     7621 acgctcgccg tcactgcgac gaactcgccg aggtgacggc cgaatccggc tacgacgcct
     7681 tcatgcggtc cgatcagtca gtcgtgttcg ggcacgaatt ctgcggcgag gtcgtcgatt
     7741 tcggtccccg cacccgcaag acaccacggg ccggcacgcc tgtggtcgca atgcgctgct
     7801 gcggcgcggc agggaggtgc acctgaccgg gttgtcgacc gaggcgccgg gcgcatacgc
     7861 cgagcagctc ttgatcgagc agtcgctggc tttccccgtc cccaacgggt tggcgccaga
     7921 gatggccgca ctgaccgagc cgatggccgt cggctggcac gccgtccgtc gcggcgaggt
     7981 gggcaaaagc gacgtcgcga tcgtgatcgg ctgcggccca atcggtctcg gcgtgatctg
     8041 catgctgaag gtgcacgggg tgcgcaccgt gatcgccagt gagctctcgg cgggccggcg
     8101 cgcgctggcg acagcatgcg gtgccgacgt cgtcgtcgat cccgcgcagg actcgcctta
     8161 ctcggcggcg gccggccacg gccacctctc cggggcgccc gacgcgctcg gcctcgcgct
     8221 cagcaccgtc gaaaaactgc acaagctgcc ggtgccctgg tggcatttgt ggcgggccgc
     8281 cgaggcggtc ggcgcggcga ctcccaagca tccagtcatc ttcgagtgcg tcggcgtccc
     8341 cggagtgatc gaccaaatca tcagtggcgc accgcttttc tcacgcgtcg tcgtggtcgg
     8401 cgtctgcatg ggcgcagata acatccggcc ggcgatggcg atcaacaagc agatcgacct
     8461 gcggttcgtg ctcggctaca cgccgctgga gttccgcgac acgctgcata tgctcgccga
     8521 cggcaaggtg gacgtcaccc cgctgatcac cggcaccgtc gggctggccg gggtggaggc
     8581 cgcgttcgac gcgctcggcg atcctgaggc gcacgcgaag atcttgatcg acccgaagag
     8641 cgacgccgcg agtccgtcgg cgtagcggct ggggagctcg cggtcgcggg atgcgaccat
     8701 ggtcgcgtga atgacatccc atcgatgggg tcgacccccg tgtacaccat caccggcgat
     8761 ccggtggcac gcgtcgccgc cgacgccacc gttgccgacg tcgccaaggc cctcgtcgcc
     8821 ggcgaggtcg gcgccgtcgt cgtcggcgac gacgagcggc ccgccgcgct ggcgagtgag
     8881 cgtgacgtcg tccgggtggt ggctgccggc aaggatccgg cctcggtgcg ggccctcgag
     8941 gtggccagca cgaaactggt gtggtgtgaa gccgacgcca ccatcgacga ggtggcggtc
     9001 cagatgatgg accactacat ccgacacatc ttggtggacc gggacggcgc tctggtcggc
     9061 atcgtttcgg cccgcgacct gctgggggtc tactgcgccg aggctgacct gggtcgcggt
     9121 tcggcgtgaa gctgcgatcc gcggccgccg cgatcggcgg aggtctggcc gggctcgcgg
     9181 gctgggacct gctgcagcgc cggcatgcga tcctgcgcaa ctacccgatc gtcggccacc
     9241 tgcggttcat cctcgaaggc gtcggccccg agctgcgcca gtacatcgtc accgacaacg
     9301 acgcggagaa gccgttcagt cgtgaccagc ggcgctgggt gtacacgtcg tcgaagttgc
     9361 gcaaccgcta cttcgggttc ggcaccgaca acgacctcga acgggtccac aactacccga
     9421 tcatcaagca cgcggcgttt cccctgtcga ctccggacgg tgaacctacc catcccgacc
     9481 cggtcgtgcc tctgcccgcg gccaaggtgc tcggcggagc gtgcaagcgc accaaggcat
     9541 ttcggccgtc gtcgttggtc aacgtctcgg cgatgagctt cggatcgctg agtggcgcgg
     9601 cgatcaccgc cctcaacaag ggcgcggcga tggccggggc gctgcacacc accggcgagg
     9661 gcggggtctc gcctcaccac ctcaacggcg gcgacctcgt ctggcagatc ggcaccggct
     9721 atttcggctg ccgcaacgac gacggcactt tcagcctgtc ccggctgatc gaccaggtcg
     9781 ccgccacccc gtccatccgg gccatcgagt tcaagctcag ccagggagcc aagccgggcc
     9841 tgggtggcat gcttccgggt gtcaaggtca ctcccgagat cgccgccatg cgcggcgttc
     9901 cggtcggagt ggactgcagg agccccgccg ggcactcggc gttccgcgac gtcgacgggc
     9961 tgctcgagtt ggtggagacc gtggccgccg cgacgggcct gcctgtcggc gtcaagtcgg
    10021 cggtcgggga ggcgacgttc tgggtcgacc tggcttcgcg catggcccgc accggtcggg
    10081 gtgtggattt cgtgactgtc gacggcggcg agggcggcac cggcgcggcg ccgctggtct
    10141 tcagcgacca cgtcgcgctg cccttcaaat gggcgttccc gcgcgtctac cgcgccttcg
    10201 ccgaacagga cctgcaccac gaggtggtgt tcgtcggatc gggaaagctg ggcatcccgg
    10261 agaacgcgct gctggcgatt gcgatgggct gcgacatggt caacgtcgcc cgcaccgcca
    10321 tgttctcgat cggctgcatc caggcgcagc gctgccacac cggccgctgc ccgacgggcg
    10381 tcgccaccca gtcgccgtgg ttggagcgtg gcctcgaccc ggccctgaag tcggtgcgct
    10441 gcgccaacta cctggcgacc gtgcgcttcg aactgctgtc gctggcgcgc gcgtgcggtc
    10501 aggtccaccc ggcgctggtg cccctcgacg cgatcgagct acttgacgtc gacctgagga
    10561 cggtgcgggt cgacgagttg ttcgactacg agcccggctg gggcatgccc ggcccggcgg
    10621 atgtctcggc gatcacgggg ctgatgggcg gctgaattcg cagcttcctg ttcgcgactg
    10681 tgcggtttcg tacacgacac gccgcgcggc gcgcccaaaa ccgcacactc ggggtggctg
    10741 cacggcgagc gacgatcacg ccgaaggatg ctgagcgaca acgtcttccg tgcgcggaac
    10801 atagtggtcc agtcgaatgc gactttcgcc cgaccagcgg taggccacca gcgttccgcc
    10861 cttcactgcg ctgaagtcga cgcacgcgct gtgcgcagtc cagtccagca gatgctcggg
    10921 cgttccctcg cgccagtagt gtccgtagaa caccggcacg tcgtctgtgt acacgtacga
    10981 ccgctcgtcg gccggcacct cgatgccggg gagttccgga tacggcgcac cgtcctccgt
    11041 cctcagcttg ccgcccatct cggcgagcgc gtgcagcgtc gtcgcgcctt ctttccacca
    11101 ccgcaccctc gcctctgtac gccggtgccc gtccttgtcg aggtacggcg gctgaccgcg
    11161 gtcgacgagg ctgatctcgg gtcccttcag caggatttcg acggcgtcgt agagtcgctc
    11221 ccccttggtg gacgctcgga cgagctggtc gagcgtgttg agccggttcg aacctagttc
    11281 ttgctcaaca actttcatcg atttctcgtg ccagcaggcg tgcacgatcc gcaggtcgcc
    11341 cagatccagc cagagcggca gggtcttgaa ccactccagg tagtacgcgc gctgctcacc
    11401 ggtgacctgg tcgaggaact tctggtgctg ggcctggttc ttctcgttgt ggggtcgcaa
    11461 gtattcaccg ctacgctcag ggtgctcggt gtgataggcg atcgcgttga attcgtggtt
    11521 gcccatgacg atctgggcgc tgccgctgtc gaccatgggc ttgacgatct cgagcactcg
    11581 cagctgttcc ggtccgcggt cgatcagatc gccgacgaaa accgcgcgtc gctccgggtg
    11641 ccggtacgcg ccggtctcat caggcgcata gccgaggtcg tcgaggagct gctccagttt
    11701 cgtggcgcag ccgtggacgt cgccgatgat gtcgtatccc ttcacgcctt catcttcgca
    11761 ccggggtacg acagcggtta ccgacctgcc tcgccttcgc aacggttcca taaccgctct
    11821 gattgtttga agcggcaaca cgttagtttg agttgagaca tcgacagaga aacgtggaag
    11881 gccttgtcgc ggaacgtaaa tcgcatttgt atcgtcacct ttgcgacggc aggcctgttt
    11941 tgtgccgcgc ccatcgcggc cgctgacccg gcagggtcga caagcccggg ggtaccgtgc
    12001 ctggagatag cgcaggagtt tgccgcctcg ccgcccgaca tccaacaacc gctgcaggat
    12061 gccgccgcgg cactggtcga gaacgcgctg ccgccgtccc tgccgtcccg gacgtcgcca
    12121 gcctgggaag cctggcgccg tcacaagtga gcctgccgac cgttccgggt ctcccgttgc
    12181 agctgcccag cgagctctcg atgcctcacg acctcatctg cgagggaacc gcatggtcgg
    12241 cgtcgaaacc aggcgccaag acgcgccctc tcggctccac gcctgcgaat cgtcggtggt
    12301 gaccggcaag ccgacggatc aatcgaccca aaccgccctc ggtcaacacg ccacggcacg
    12361 atactttcgc aagggcatgc gtaaagcgac gcgaagggaa gtgtggcggt gagccgagta
    12421 ctggtgggtt cagcagcggt ctgcgcgttc gcgggcctga tcggcggcat cccggccgca
    12481 agcgcagatc cgtttccgtc gtgcggcccc gaccaagcca ccgccatcgg atcggcaacc
    12541 gcccgcgacc gacatgacac gatcacccaa gcgccctgga accctgtccc ggtccagagc
    12601 aattacgacg cctgcgcgga cctgtcggcg gccgtcatca cgatcgacat ggccaggccg
    12661 acttcgcccc gccaggtttt cctgttccac cgcggcacct acatcggcaa cgcgaccaac
    12721 aattcgcggc cgtttaccac gctggacgcg gcggcgtcca caccgaacac ggtggtgctt
    12781 gatttcgttt ccggccgcac gtgcggcacc tgcgacgacg gccagacata tcccgtccgg
    12841 tattactgga acggcgtcac ggccgtgatg cccgatccga ttccgccacg ccaggattgg
    12901 ccaatcccgt agatacgtgc ggatgaccgc gccgacgacg tggcgcctgg ccgacggccg
    12961 cgaccttctg ttcttctcac tgcccggtca cactccggct ccggtcaccg accggcggcc
    13021 gctgcccgaa cgcgaccgcc atcagtccca gttacgcctc gaccggcaga ccggccagtg
    13081 ggtcacggtc gcgccgcgcc gtcagaaccg cacctacaag ccgggcgccg acagttgccc
    13141 gctgtgtccg gatccgtccg gcctgacgac cgaagtgccg gccgccgact atgacgtcgt
    13201 cgtgttcgag aaccgctttc ccggtttcgc cggagctgcg gacgggacgc cggtaatgac
    13261 cgacgacctc ttcgcttccg tgcccggcca cggccactgc gagatgatct gcttctgcag
    13321 caaccacctc ggctcgttcg ccgatctcga tcattcccac gcgcgactcg tcgtggacgc
    13381 atggcgtcac cgcaccgcgg acctgatgac gcgacccggg atcgagcaag tgttctgctt
    13441 cgagaaccgc ggcgaggaga tcggcgtgac gctcacccat ccgcacggtc agatctacgg
    13501 ctacccgttc ctcaccccgc gcaccgcgga aatgcttcgg caagcccagg cgtaccgatc
    13561 ggcgagtggc cgcaacctgt ttggcgacct cctcgcgcag gagctctccg acggcagccg
    13621 cgtcgtcacg cacaccgaac tcttcaccgc cttcgtgccg ttcgccgccc gctggccagt
    13681 ggaagtgcac atctacccga accggttcgt gcacaatctt gttgacctcg atgacgccga
    13741 gctcgacgag ttcgcgcagg tctacctcga catcctcaac cgcttcgacc ggttgtattc
    13801 gattccgctg ccgtacatct cggcgctgca tcagtacgcc gacgacgacg tgcagcgcga
    13861 cggctacttc cacgtcgaac tgatgtctat acgacgcacg gccaccaagc tgaagtacct
    13921 ggccgcctct gaatcggcga tgggcgcgtt catcagcgac gtgacacctg aaagcatcgc
    13981 agcacgccta cgcgaggtct gacgtgatca cgtacggtgc gcccggccgg atcaacctga
    14041 tcggcgaaca caccgactac aacctgggct tcgctctgcc catcgcgctc cccgaacggg
    14101 ccgtagtaac ttattcgcca agtgattccg acgctctcaa cgtgcgcagc gacgccgaac
    14161 ccggcgaggt tcgcatcccg ctcgacaccg cacccggtga cgtgacgggt tgggccgcct
    14221 acgtcgcggg ggtggtctgg tcgctgcgcc gcgcagggca ccgcgccgtc ggcggcgaca
    14281 tgtcgatcga gagcggcgtc ccgataggcg cgggtttggc gtcgtcggca gccctggaat
    14341 gcgcggtttt gggcgcgata ctcgaagcca ccggcgcgcg cgtcgaccgt ctcgagcaag
    14401 cgcgcatcgc gcaggccgcc gagaacgact acgtcggcgc cccaacggga ttgctggacc
    14461 aactcgcctg ccttttcgcc gaaccgggcg cggcagtgct gatcgacttc cgggcagtcg
    14521 ccgtgcaact ggtgcccttc gaccccgacg cgtccgatgt cgcactgctg ctggtcgact
    14581 cgcatgcccg gcaccgccac gccggcggcg agtacgccgc gcgccgcgcg tcgtgcgaga
    14641 gcgaggccgc cgatctgggc gtggcgtcac tgcgcgacgt gcaggtaccg cctcgcaacc
    14701 gatacgcgcg tcacgtcgtg accgagaacc agcgcgtgct ggatttcgtt gcggcactgt
    14761 ctgattgcaa ctttgggcgg gcgggtcacc ttctgacggc ctcgcatgcg tcgatgcgcg
    14821 acgacttcga catcaccacc gaccacatcg acctgatcgc ggcgaccgca ctgcaggccg
    14881 gcgcgctggg cacacggatg accggcggcg ggttcggcgg ctgcgtgatc gcgttggtcc
    14941 cgaccgacga gatcgaccgc gtgggtgatg cggtgcgacg cgcgatccgc gatgccggct
    15001 acccagagcc gacggtccga cacgttcgcg ccgggcgcgg tgcatcggcc gaggcatgac
    15061 ggccgctacg ctcgtgcggc atggcgacct caaacctcag ccgctccgtc gcggacagaa
    15121 cggctttcat caccggcgcg gccagcggca tcggccgggc ggcggcacac gtcttcgccg
    15181 ccgaaggagt gcgactggcg ctggccgaca ttcagtcgca ggcagtggaa caagtcgctg
    15241 cggcagtggg tgcttccggc gggcaggctc aggcctggac cctcgacgtg gccgatgccg
    15301 ccgccgtcgc ctctaccgtg ccgcgggccg cggacgcgct cggcggcttg gacttcgtca
    15361 tcaacaacgc gggcgtcgtg cggcccacga acctggacga cgaaggctat gacgaactgt
    15421 ggcatcagca gctcgacatc atgttgaccg cgcaccaacg gatcgttcgt gccgcgctgc
    15481 cgtatctgcg tcgatcgtcg tgcccgcgca tcgtcaacgt cgcgtcgacc gaggcgatgg
    15541 gagcgacccg cggcaacggc atctacgccg tcgcaaagca cggggtggtg ggcttgacgc
    15601 gtgcgctcgc ggttgacctt ggccgcgaag gcattaccgt caactgcgtc tgcccgggtc
    15661 cgatcaacac cgcgctcacc gaaccgatcc ccgacgagca caagatcatc ttcgcgaaac
    15721 gccgcacggc gcttggtcgc tacggcgagc ccgccgaagt cgctcacctg atcctgagca
    15781 tgtgcctgcc ggcggcctcc tacgtcaccg gcgccgtgat tccggtcgac ggcggtctga
    15841 tggcgcgcaa cgcctgatcg cgttggagaa ctgcaagatc tgacggtcgc gctaccgcgg
    15901 cgggcggaac tccgcagcga tcgtcgtgcg gatctcgtcg agcaccctgg cgcgcgccct
    15961 caacttgctt tccttgtgag cgcgcgcgtc gaggctcgta aatcctttgg cgacttcatg
    16021 agcgcggggc cgcaccatgt cagcctcgac cagttcgtcg agatagcctg ccgcgacagc
    16081 actttcaccc ttgaactccg ccgacagtcc aaccgcgcgc tgaaatgccg ccggggtcaa
    16141 ccgatgccgc aggatcgcca gcgcgggacc cggcatcgtc agcccgatcg acacctcgtt
    16201 ggcctgcacc cgatgcgagg gggcaccgat caggtggtcg ccgcagagca tgaggaacga
    16261 ccccatcgcg atcgagtgtc cggtgcacgc catcaccacc ggcttgggat aggcgagaat
    16321 ccgcgcggcc agctcgaacc cgcccgcgag catctcgatc gccgcgtcgc cgccgccggt
    16381 caacaccttg aggtcgaagc cgccgctgaa cacccggttg ttgccggata tgacgaccgc
    16441 gccgacacct tcgagctcgg cgtggtcgag cgcctcgttg atctcgagct gcatgtccgg
    16501 cgacagtgcg ttgaccttgc cgtcgtccat cgcgatcgtc gcgaccgact cgtcgacgct
    16561 gaagtgaacc cgttctccca tgcggactga cgttactcag gcgcccatga gcatccgcca
    16621 ctgctccaag ctggtcgggc ggaagacgta gttggtgcga cggacctccg acaacgacgc
    16681 gctgggttct tcggaatacc agtggccggg gaagatggtc ggatcgccgg gcaactgcgc
    16741 gagctggtgc aggctgcggt acatctcgtc gacgtcaccg cccggaaagt cggtgcggcc
    16801 gcagccttcc aggaacaggg tgtcgccggc gaccaggcgt ccgtcgagca ggaagcactg
    16861 gctgcccggg gtgtggccgg gggtgtgcag cagttcgatc tcgatgtcgc cgatgctgac
    16921 cttgtcgccg tgctcgtgct tggtcaggtc gttgagcggg atgccggtca cccgcgacac
    16981 ccacagcgct tcgtgggtgt tgacgtggac ggggacttcg gtccggtcca gcagctcggc
    17041 gaggcctttg agctgaaagc ccatcatcgt gccgccgacg tggtcggggt ggtgatgggt
    17101 gacgagcacg ccggacaggt gcatgccgtc ttgttccagc gcgtcgacga ggtttgttgc
    17161 ggcgtatgcc gggtcgacca ccacgcagtc tttcgtctcg cggtcgccga tgaggtaggc
    17221 gaagttgcgc atctgccgtg caatcatgtc gctctgcgcg aagtcgcggc cggagagcag
    17281 ctggcggaag tacaggcgat cgtcggtggc catggcttca gcgtatgagg tgccagtcgt
    17341 cgaggcttca ggaggacagt actctttgcc cgcctcgtag cgacaacagc gtttccccag
    17401 ttcaggggcg taacgaggag agagcggtca gcgacaagac cgcccttccg ccgcaggtcc
    17461 acgagctgtc gctcacgagg cgggcaaaga gatatatccc ttggattccg ccagaagcgg
    17521 agacgactcc gcgaccggac gccgggcgtc acgtctcgct gtgcagcttc accatccgcg
    17581 tcgtggtgct ctcgtcgagg tcgacgatct cgctgcgaga cctcaggaag tcactgaaag
    17641 agcggaaacc caaagatttt tcgctgaacg acgggtccat gcgcttcatc tgggccttga
    17701 ccgctgagtt gtgcagccat tcggtgtcgt ccttctgcag ccccatccgt agcgcccgct
    17761 ccaagaggtt ggtggcggcc agttgcacgt cctgcgacgg agtttcgtcg tcgggctctt
    17821 gcgttgccgc gcgacgcgac cgcttcttcg gtgcgtcctt cggcagactc ggcacaccgg
    17881 gaagcgcgtc gtaggtgacg aactcgtcgc aggcggcggc cagcgaaccg ctgctcgccc
    17941 cggcgacccc gatgccgacg acgtaccggc cgaggcgctt gcagcgctga gcgagcgcaa
    18001 tgtagtcgga gtcgccgccg acgatcacga cgtgcgtcag atcgggcagc cggaacatgt
    18061 cttcgactgc gtcgaccgcc agccggatgt cggcgccgtt cttgccgtag gccgcggccg
    18121 ggaacaactg cacaaggtcg accgcacgcg cgaccagctg gccgctgtaa ccggcgttga
    18181 ggtcagcgga ccagtccgcg tacgcgcgcg tgagcaccag cgtgccgaac gacgaagcga
    18241 agtcgatgat cgcgccgacg tcgacggtcg cccgcgtgag gctttccgcg tccagcccct
    18301 tgctcttgtc tttctggaac gaattgcgac cgtgcacctg gtcgtaccgc gagatcacga
    18361 tgttgtcgaa gtccatgtac accgcgaccc gagtcgcgcc ttcagtcatg gcactcaatc
    18421 taagtagcga caatgggccc gttgcgaaat ggcggaaatt gtcgctggaa ggcgggcaat
    18481 tccactacgg cccttctcgg aggcatgtgg ggctgaagtg acacttgttg tcacgctggt
    18541 cgcatatgtc acagttttcc ctgcggcggg aggttatgtg tttgcccggc tctcagctct
    18601 cagcgacaac ttctccgcct cggcccgcgg cgcgcgcgtt ctgtcggcca ggcatgtcac
    18661 tgtcttgcca tgacgactcg tcgatgcctg cggggaaccg aactgcgcta cgtcctcact
    18721 caccatctgt tccttcacgg gtctgcgacc gtcgtggaat tgatcgacat gctcgactgg
    18781 cacggcttcg agacccgcgg gcggccgtcg aaggcgatat ccgatgcgct gcgttgggag
    18841 atgggacgag gccgagtgtg cagacgaggc cgcggcctat acgtcccggt ttggatgccg
    18901 cgggggaccg agcatcgcat ccacgcgcgc gggctggcgc tgcgggccga agcggagcgc
    18961 tggcgccgcg acagcgccta gacgggccca acctcgtgtt tggcgggcca gccagccacg
    19021 ctgtaccctc taacgggccc ttcgcgcggg tcaggccccc ttagctcagt cggcagagcg
    19081 tttccatggt aaggaaaagg tcaacggttc gattccgtta gggggctcgg tggacgccca
    19141 ggcgatgtag ctcagtcggt tagagcgaac gactcataat cgttaggtcg ccggttcgag
    19201 tccggccatc gctacaacac aacaagcaac aagaagggca actgacgtgg cctccagtac
    19261 ggacgtacgg ccaaaaatca ccttggcgtg cgaggtgtgc aaacaccgca actacatcac
    19321 caagaagaat cgccgcaatg atcccgaccg gctcgaactg aagaagttct gccccaactg
    19381 cggcaagcac caggcgcaca aagaaacccg ctgaccgcgc gccgattagc gattattcag
    19441 cttgactagg taggttcacc acccgtgtct ttgtccgaaa agatcgtcgg gatgcattac
    19501 cggtatcccg actactacgt ggttggccgg gagaaggtcc gcgagtacgc caccgccgtg
    19561 aagaacgacg atcccgcatt cttcgaggag aaggccgccg ccgagctcgg ctacgacggg
    19621 ttgctggcgc cgctgacgtt catctcggtg ttcggttacc aggcccagtc tgcgttcttc
    19681 gccagcgcaa acgtcacggt caaggacgcg aagatcgtcc aagtcgacca ggtactgaag
    19741 ttcatgaggc cgatcacggt gggtgaccgc ctctactgcg acgtctacgt ggactcggtc
    19801 cggcagtcct tcggcactga cacgatcgtg acgaagaaca tcgtgacgaa cgaggacggc
    19861 gacatagttc aggagaccta cacgaccctt gtgggtcgca gcgagggaga gggagaagag
    19921 ggcttcagcg atgccattgc gtgagttcga ttcggtgaag gtcggcgacc agcttccgga
    19981 gaaggtgatc acgctgaccc gccaggacct ggtcaactac gccggcgtct ccggcgacct
    20041 gaacccgatc cactgggacg acgacatcgc ccagcaggtc ggtctcgaca cggcgatcgc
    20101 ccacggcatg ctgaccatgg gcctcggcgg gggctacgtg acgtcgtgga tcggcgaccc
    20161 cggcgccgtc acggagtaca acgtccggtt cacggccgtg gtgccggtgc ccaatgacgg
    20221 caggggcgcc gacattgtgt tcagcggacg cgtgaagtcc gcggatccgg aaaccaagtt
    20281 ggtgacgatt gcagtggcgg ccacctccgg tgggaagaag atcttcggcc gcgccgtcgc
    20341 gaccgcgaag ctggcgtaac cccatggctc tcaagaccga catccgcggg aagattttca
    20401 agtaccccga cttcttcgag gtgagccgtg aacagatccg ccagtatgcg agggccgtca
    20461 aggcggagga tcccgccacc ttcgacgagg acgaggcaca cgccctcggc tacgacacga
    20521 tcgttgcgcc gctgacgttt tcggcgatct tcgcgctgct gatccagcag cacttattcc
    20581 ggcaggtcga cattggcatg gagaccatgc agatcgtcca agtcgaccaa aagttcctgt
    20641 atcacaaacc catccaagcc ggcgacaggc tgttcgggac cgtgtacgtc cactcggtag
    20701 acgagcggtt cggtgccgac atcgtcgtga cgagaaacgt ctgcgagaac gaaaacggcg
    20761 aagtggtgat ggaggcgttc accacgttga tgggtcagca gggcgacaac tcgacccaga
    20821 ttaagtggga cgctgagtcc ggccaggtga tgcggaaggc cgtcggcgag taatcctcgt
    20881 tgggattagt aactccgatg tgcgccgatg tacactcgga cctcggggtt tccccacttc
    20941 agcgcgctgt ccgtcggtga gaaatcgacc gcacggggag cgcgcgaatt gtgtgggccc
    21001 cggcgcgagc aggttggcct gccaacctca aggggcgtag ctcaattggc agagcagcgg
    21061 tctccaaaac cgcaggttgc aggttcaagt cctgtcgccc ctgctgaact gaatacggat
    21121 cgatcgtgga cactggatgg tggacacaca caacacacga gcggaagggc atgcggtgag
    21181 cgacgagcgc gaggctgccg gcgccgcagg cgacggcgag gacaccacga ccagcggtgg
    21241 tcggaccgcg gtcgccaccc ggccgctgcg tcccaccggc aagcggtcgc ggcaggtgac
    21301 cgagcccgtc gaggccgagc cgatcgagaa ggccgagaag accaaagacc gcggcaagaa
    21361 gaaggccaag acggccaaga agaccggccc agacaagggt cgacccaacc cgatcgcgtt
    21421 cgtcgtgaac tacctcaagc aggttgtcgc cgagctgcgc aaggtcattt ggccgaaccg
    21481 caagcagatg gtcaactaca ccgctgtggt gctggttttc ctggtcttca tggtcgcgct
    21541 gatctttgtg gtcgatgtcg ggctcgcggc gctcgtgcgt ggtgtgttcg ccggcgaatg
    21601 ggcattgagg tttaggtagg gaaggacaaa gaccgtgaca accccagacg gcgaccaggg
    21661 cttctctgac gagcagccag ccgccgaatt gcctgcggcg caggccgtcg atgtgattga
    21721 tgacgaggcg gccgatgtgg ccggcgaggc gaccccggaa gaagagctag acccagccga
    21781 agcgctgaag gcggacctgc gcaccaggcc cggcgagtgg tacgtcgtgc acagctacgc
    21841 cggctacgag aacaaggtga aggccaacct cgaaacccgg gtgcagaacc tcgacgtcgg
    21901 cgactacatc ttccaggtgg aggtgcccac cgaagaggtc accgagatca agaacggcca
    21961 gcgcaagcag gtcaaccgga aagtgttgcc cggctacatc ctggtccgca tggacctcac
    22021 cgacgactcg tggtcggcgg tgcgcaacac gcccggcgtc accgggttcg tcggcgcgac
    22081 ttcgcgcccg tcgccgctgt cgctcgacga cgtggtgaag ttcctgctgc cgcagggtgc
    22141 cgcgaagaag acgacgaagg ccgcggccgc cggtgccgcc gccgagaccg gcggaattga
    22201 acgtgccccg atcgaggttg acttcgaggt cggcgaatcc gtcaccgtca tggacggccc
    22261 gttcgcaacg ttgcccgcct cgatcagcga ggtcaacgcc gaacaacaga agctcaaggt
    22321 gctggtgtcc atcttcggcc gcgaaacacc tgtcgagttg accttcaccc aggtcgccaa
    22381 gatttaatgc tcttcgcgca agcggctcat cgttctagaa aggaacacca acacctcatg
    22441 gcaccgagaa aaaaggtctc tgggctgatc aagctgcaga tccaggcggg ccaggccaac
    22501 cctgcgccgc cggtcggtcc ggcgctcggc cagcacggcg tcaacatcat ggagttctgc
    22561 aaggcctaca acgccgcgac cgagaaccag cgcggcaacg tcgtgccggt ggaaatcacg
    22621 gtctacgagg accgcagctt cacgttcaac ctgaagacgc cgcctgcggc caggctgctg
    22681 ctgaaggccg cccgcgtcga caagggttcc gctgagccgc accgagtcaa ggtcgcgaag
    22741 gtgtcgtggg accaggtccg cgagatcgcc gagaccaaga aggacgacct caacgccaac
    22801 gacatcgacg ccgctgccaa gatcatcgcc ggcaccgccc ggtcgatggg catcacggtc
    22861 gaatagatca catctgtggg agggcccgct tcggcccggc gatgagcgct cgcgcgaaga
    22921 gcaatcatca cagcaccaca actccaacaa cacgattgga agaccaatga gcaagaagag
    22981 caaggcatat caggcggccg ctgaaaaggt ggaccgcagc cagctgtacg cgccgatggc
    23041 cgcagtgaag ctggccaagg aaacgtcgtc gaagaatcag gactcgaccg tggaggtcgc
    23101 gatccggctc ggcgtcgacc cgcgcaaggc cgaccagatg gtgcgcggca ccgtcaacct
    23161 gcccaacgga accggcaaga ccgcgcgcgt cgcggtgttc gcggtcggcg acaaggcttc
    23221 tgaggccgag gccgccggtg ccgacatcgt cggtagtgac gacctgctcg agcggatcca
    23281 gggcggcttc ttggacttcg acgccgcgat cgccacaccg gaccagatgg ccaaggtcgg
    23341 tcgcgtcgcc cgcatcctgg gcccgcgcgg cttgatgccg aaccccaaga cgggcaccgt
    23401 cacgccggac atcgccaagg ccgtcgccga catcaagggc ggcaagatca acttccgcgt
    23461 cgacaagcag gccaacctgc acctggtgat cggcaaggcg tcgttcgacg agaacaagct
    23521 cgtcgagaac tacggcgccg cgatcgacga ggtgctgcgc gccaagccga ccgcgtcgaa
    23581 gggccgctac ctgaagaaga tcaccgtgtc gacgaccacg ggcccgggca tcccggtcga
    23641 cccgtcggtg acgcggaact tcgcggaggc gtaagtgaaa ccggagccga ccatccgcac
    23701 cgaggcatag cccttctcaa tgggccagct cggagtactc ggtcatgtgt tgacgctcgg
    23761 cttggtcttc ctggtgggga tcggggtcgc gctcagcgcg gtgttggtag cggatcgcgc
    23821 gctcggtaaa tcggttggtc cacagcataa ttcgacgctg tcaccgttca tgacggttgt
    23881 cggacttgtc ttcggggctc tcctcggctt caccgttgtc gtttcctggg agcagttttc
    23941 ctccgccgag gcgaacgtcg ctcacgaggc gtccacccta acgacgatgt accgacagac
    24001 cgtcggcatg cccgacccag agcagacgaa gctgcgagag cagctgcgga agtatgccga
    24061 agctgctaag ggcccggatt gggaggccct cgacagcagc ggcaagggcg acggcgcacg
    24121 aagcgcgatc acgcagatgt accgcctcct cggtaatcag cccgacgcct cgtcgaatcc
    24181 ggtcaacggg gaattcctca gccagctcac cgtcttggcg aacgaccgaa atcagcgcgt
    24241 gttggacgcc caaccgcgaa taccgggact gttgtgggcc ggcctgctgt tcggcggcat
    24301 agtgctggtc gcgctgacgg gcttcatgcg cctggacaac aggctcggtc acgcgatcct
    24361 gtcgagcgcg atcgcggttc tactcggctt gctgctgtac atcatctatt ggctggacca
    24421 tccgttgggg acggacatcg gtgtcacgcc agtaccgttc cggcactcgc tggaagtgtt
    24481 cgacatcatc gaccgtggga cgtagctagg ccagcgactt ggcgaacccg ctgatgtcgc
    24541 ggacggtcgc gccgtacacg tcgggcagcg cgccggcgca gagcatatcg ccggcatggc
    24601 acgtgccggc gatgatccgg ccgagcgtgg gaacacccgc gcgcagcagc gcgcggtagt
    24661 acgccaggcc ctcgtcgcgc atcggatcga gttcgttgac ggagatcacg tgcggcggca
    24721 tgcccgcaag gtcggcctcg gtcgcatttc cgggccaaca tgtggggtcg ccgctgtgtt
    24781 cgccgtcagg gtcgtagacc gaaccgatga cggccatctc catggagttg aagatgtagc
    24841 cgtcgttctc cttcagggac ggcaggtcgt cgggctgttc gaggtagcgg ttcgaaatgt
    24901 acgggcattg cgcgtagacc ccggcgattt catgcagcca ggcttcgcgc ttggccttgt
    24961 gagcaacggc cagggtgaga tttccgccac ccgattcgcc cgacacgacg atatgcgaag
    25021 cgccgagatc gcccagatga gcagccgtcc agcgaaggcc ggccgcacag tcgttcagac
    25081 ccgctggata gggatgtggg cccaattcgc ctgcgctgtt acggaattcg acgccgacga
    25141 ccaccaggcc ggtggcggcg agattgtcac gccagcggac gtaaccgatg tcggtggcgc
    25201 tgccgaacgc catgccgccg ccgtggatgt ggaatatgca cggggctacc gcggtcgtgc
    25261 cgctcggtcg gctgatgtac agcgtgatgt cgtttccgtc gtcgccgcgg atcgtcgtgg
    25321 tctcgttcgt cacggcttcg gcgaccggcg cgatccgggc caacgcgtcg aggacatcga
    25381 cgagaacttc ttctgcctct gtgacatatg cgacccggtc cggcagtggt gagtccggcg
    25441 tcaccggagg gagcggcaga aggccgtcaa gaccgaacgg tgccagcgct gcgatcatgc
    25501 gcgggtcgga ccgcggatcg gtgccgagtg tgcaatcggg attggcgagg cggccttcag
    25561 gcataggccc atgctaggcg tcgcctcggc cgccgcattc ggcgattcgc cgccgcagga
    25621 acgccttcgc cggctcggaa cccgtcaccg ccagggcctc ctgataccac ggcagcgcct
    25681 cgtcggcgcg cccgtcgcgg cgcagcaggt ccgccctgac tgaccggacc aggctggacc
    25741 gcaccaaccg gggatcgccg gcgacctcgt caagcgcctt gaggcccacg gcgaacccgt
    25801 cgcgaaaccc aagcgccaca gcgcgattcg cccgcacgac cggggaatcg ttgaccgcca
    25861 gcagtcggtc gtatgccgcg cagacacctc cccagtctgt ttgctcgaac gtcggcgccg
    25921 tggcgtgcag cgcggcgatc accgcctgcg gcaggtacgg gcccgtcgag ccggccgccc
    25981 gccgcacccg ccgcagcccc cgcgcgatcg ccatcgcgtc ccagcgggtg cggtcctgct
    26041 cgtcgagcgg caccagcgcg ccgtcgccgt cgacgcgggt cagccgccgc gcgtcgtgca
    26101 gcgtcaccag cgcggacaga gcctgggtct cgggttcgtc gggcatcagc ccggcgagtt
    26161 cgccggccag ccgcacaccc tcggtgcaca gttcgtcgcg gatggccgac ggacccgccg
    26221 tggaccagta gccctcggtg aacaccgagt agatgcagcc gagcacgtgc ggcgtgcgct
    26281 ccggcagccg ctcggccgaa ggcaccttca gcggaatccg ggcgtgccga atcttgctct
    26341 tggcccgggt gattcgctgc ccgatcgccg cctccgtctg cagcagcgcg cgggcgatct
    26401 cggggacggt caggccggac accagccgca gcgtcaatgc caactgcgac gtccggtcca
    26461 gcgcaggatg cgcgcatgtg aacatcatcc gcagctcgtc gtcgcggacg gggtgcgggt
    26521 ccgcggtgtc ggtccgcgcc ctgatgtcgt cgaccacggc ggccaattcc tttccggggc
    26581 ggacggattc gcgacgtaac cgatcacggg cccggttgcg cgcgaccgtc accacccacg
    26641 cgcccggatt gtccggcacg ccgtcacggg gccacgcccg cgccgcctcg gtgaacgctt
    26701 cctggacggc gtcttccgcg acggtgagat ctcccgacca gcgggccagc gccgcgacgg
    26761 ccggacccca ttcgcgccgg aagacgccgt ccaggtctct cacggactac agcccggcga
    26821 tgcccatcag ctggcgcagc tccacggtgg tcgccgggat catcgaggcc agcttcaccg
    26881 cctcgtcacg atcggccgcc gagagcacgt agaagccgtt ggccacctcg gtgccctcga
    26941 cgaatgggcc gtcggtgagt aacacctcgt catcccgcag ccgcaccgtg gtgacggtgg
    27001 acggctcgtg aagcgccgcg ccaccgatga tgtgcggacc ggcggcagcg ccgaacgcgc
    27061 cgtgcttcgc cgctccggcc tcccactccg gcgtgccggg tgccgacacc gttgccggcg
    27121 gctcgagcag cagggccagc cagtgctcgt cggtcagctt cgtggtgggc gtgaactcca
    27181 tggccatcgg ccagacctcc accgctccct tgcgggcgtg cgggatctgc cgcgtcaggg
    27241 ccagcgcggc gtcgaggtcg tcggcctcga acacgtagaa gcctcccgcg atctctgcgc
    27301 tctccgggaa cgggccgtcg gtgaccacgg gcgcgtcgcg gccgccggtg attcggacgc
    27361 ccgccggggc aggggtcagc gcatcaccgg ccttgatgga cgacctctcg cgggcatgga
    27421 agtcgttgta tgccgccatc tcggcctcca atgcggctgc gtctgcgcgc tcctcgggcg
    27481 cgtcggacgg cgcgaccaac agggcgaagt actgcatggt gtcacctctc cgggtagcga
    27541 atggaacccc gtgttccact ctctactcct ccgacgaacg gcgccgccgc aatccgacag
    27601 gttgttgaaa gcgaaaagtg ctagcaaacg caagaaagtt tgccaatccc ctaccgctgg
    27661 acatcgcgtt gtgtttttgt ttacggatgc gtaaaccggc tcgggcgata gtcgtcgctg
    27721 tcctgacgtt cctcagtgcc gtcgtgatcg gtgcagcagc aattctgacg gcggcgatca
    27781 cactggccgc ggtaagcgcg gtgggattgg tcgtgccggg cacgggcacg cccaacgcca
    27841 acattgtggc gaactacaag gagaacgccc gcgaccgcta catgacgaca acgccgtgca
    27901 cggtggccaa cggctgcact ggcgccaacc tcatcgggat caactacccc gcgtcgttct
    27961 ggccgctagg gttccctccg ttcccctcga cctggtgccc gggcttgaaa tgcgacacat
    28021 ggaacgtatc ggccggggaa ggcttagcgc atctcaaatc cgcactgatc acagtgctga
    28081 gcgacccgac aaatccagac atagtcatct tcggctactc acaaggcggc gcagttgtct
    28141 ccgccgagat gtacaacctc gccagcctgc cgcaaaccac gaaggaccgc atcaaggtgg
    28201 tcacgatcgg caacatttca aacccgcaag ggttgtgggc gcggctcagc ttcctgccga
    28261 ccatccaaat tctcaacgtg acgttcgggc cactgctgcc cacagacatc ggtatcgaga
    28321 gcactaacta cgtgttcgag tacgaccccg tcggcgacgc tccgctgaac tggtggaatc
    28381 cgctggcgct cctgaacgcc gtgttcgcgt tgcagtacgt ccacggcaat tatctgcttc
    28441 ccaacggcaa cgacccgacc gggacgttgc cgtacggata taccccggag acgttggcgg
    28501 cggcgataca ggaccccaac aacatccgca cttaccagga cgcgaccttc gtgttgattc
    28561 cccagaaggg gacgctgccc cttttccaac cgatcgccga cctggctgca gcaaccggca
    28621 cgactccgtt cgtgaagccg atcctcgatc tgatttcgcc tgtcttcaag gtgttgatcg
    28681 atctcggcta cgaccgcacc ctcaacccgg gcataccgcg gactctcaac ctgattccga
    28741 tcttcaaccc gatcaaactc gccgtcgacc ttgtcgtcgc ggccggcgaa ggcattcagg
    28801 ctgcatggaa ggacatcacc ggcaccgcgc ccgcgctgcc ggctccgttc gcgccgagtg
    28861 gtccgtcgat cctggcggcg gacaacgctg cgaagctcgc tcggaccacg gagatcgaag
    28921 gggcgaagcc cgagtcgcag tcgccgctca agatcaatcc gcgtgacggg ctcagcccgc
    28981 tgaagattga cccgctgaag attgacccga agccggatgc cgacgccggc attgtcgcgc
    29041 cgaagaccta cccgagccag atcggcttcg acgagtcttt gaacgcggcc ttcagcctgc
    29101 cgaagacacc gaagccgggc ctttctgcgg tgccgccgct caagctgccc aagc
//
LOCUS       DASWBF010000515         4184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1102930,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000515 DASWBF010000000
VERSION     DASWBF010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102930"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..918)
                     /locus_tag="VF874_19075"
     CDS             complement(262..918)
                     /locus_tag="VF874_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6474 family protein"
                     /protein_id="HEX9178078.1"
                     /translation="MGLFSKRKSRATRRAEARALKRRAKLDAKYAARNERRRIKSAER
                     AGEKALRAQIRAQRSSDRAALKVAETNLKAAREGKVFSPTRIRRTLTVTRLLAPIVVP
                     VAYRVAVGARGWFDERRADRLGVPLTELGQFSGYGARLSARIAGAEQSVRLVAEKNPK
                     DAETKRFVAAITGRLKDLSAAVTAAENMPSARRRAAHSAIATELDGIDADLLARLGLH
                     "
     gene            1124..2335
                     /locus_tag="VF874_19080"
     CDS             1124..2335
                     /locus_tag="VF874_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178079.1"
                     /translation="MGRSLIRYSAVACVAAGGLFMGGTGAVAFADDETGGSATQTAPP
                     TTASPPDRFQLPKWLRFPSFSGTEPRVGFIPPPVMKLPAKLGSTTAPDGSTTLQAPTI
                     TLPFVIGTATKPAGPQSPGALIAPDTGTEPAGPQSPGALIAPDTSETPGVVDGTSIEQ
                     NRKPAATTQQSPIDLTRLVPPGVPTGPPMTIKNPLRELLPADLDLHQPLVRQLLPPPL
                     VEILTAVAQQIPLAGLVISPVMEFVVPPFFADVVIPMLLSDIVVPTLPLDAIFPAPLP
                     TSLPLTKAAPLALPGSGSLPPELAPMGMDVLQASASPAGPASEPTGVEKLQAPLPLPD
                     TSADPLAEVDFRAGYSDYLRNAGQAQITAIAVPGAVAILLFTAGGGFIGYRQARAGHV
                     IRAEGITRFLR"
     gene            2383..2874
                     /locus_tag="VF874_19085"
     CDS             2383..2874
                     /locus_tag="VF874_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF955 domain-containing protein"
                     /protein_id="HEX9178080.1"
                     /translation="MPASRRVARAVSAVLNMAPRHGEVSIVRLVQAVADSRDRRIDIQ
                     MEELPPGVCGQWRQYGDYDVFLIQQGLPAWDRTLAHELGHLVLGHEGISVVDAAREGT
                     ELVSEDLINYMLNQRTGCMGPAGEDAEQEAEDFAALLGYRLGRLRSDRSSIVQVRLGE
                     AFG"
     gene            2871..4019
                     /locus_tag="VF874_19090"
     CDS             2871..4019
                     /locus_tag="VF874_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178081.1"
                     /translation="MIVWVIAGLLGVATGLRVGWALVNKQSVVSAAMMLALGSLGFVA
                     ALNWQPLTLLVDTLLRWPNISVALSQIALTLCAAASCVMITSAASAKRPVVTHRLAWA
                     NYGIAAAIAIASVALFFVDGRQPEMAPQEYLRRNLNEHTSLPWLLPLLYMLLVLTVVL
                     WAGMRYSNTSRRGRALFVFSVGIGLLVLTLGFFLLRAVGSTEFVGVGSAITLLACAML
                     IVATGSLLPSVEDWFGARREMRTIQPLLRELCLRQPEVGVGVRPSGPLAFQVAEQMSL
                     ISDGLFLEATQAHDAARSGRLSPADRNTPAVSSREQALAIARWIYDSGQDADKTSGFP
                     GLAWLRQPDSYSDREWILEIARQYHDLITAGQSSVPVGEHAEVRGTTH"
     gene            complement(4032..>4184)
                     /locus_tag="VF874_19095"
     CDS             complement(4032..>4184)
                     /locus_tag="VF874_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9178082.1"
                     /translation="KLDQELTWLAEMRDEGVRRIAARTVGLSSEAKDDLVARAEELRR
                     KERLDS"
BASE COUNT          640 a         1414 c         1394 g          736 t
ORIGIN      
        1 aaggtcagca ccgcgctatc tccctgctcg gatctgtcgg cgtcgacgtg cacgtgtgcc
       61 gatgccggtg cggcgccggc gaacagcccg acggcgagga ccgcggcggt gtgatcgcca
      121 gcacggcgag cagttccgcc cccgcaccca atggggcggc tccgccggcg agccggtgtg
      181 cgcccacgga caacgcgacg gtgagcagtg ccgccgatgc aacgcgccaa cgcgcagcgg
      241 gggcggaccg gcgcatgcgg atcagtgcag gcccagtcgg gccaacagat ccgcgtcgat
      301 gccgtccagt tctgtggcga ttgctgagtg cgcggcgcgg cgacgagcgg agggcatgtt
      361 ctcggccgcg gtgacggcgg cggacaggtc tttcagccgg cccgtgatcg cggcgacgaa
      421 ccgcttggtt tcggcgtctt tgggattctt ctcggcgacg agacgcaccg actgttcggc
      481 gccggcgatg cgcgccgaca gccgggctcc gtaaccggag aactggccaa gctcggtcag
      541 cggcaccccg agccggtcgg cccggcgctc gtcgaaccag ccccgggcgc ccacggccac
      601 ccggtacgcg accggcacca cgatcggtgc gagcaaccgc gtgaccgtca gcgtccggcg
      661 aattcgcgtc ggcgagaaca cctttccctc gcgggccgcc ttcaaattgg tttcggcgac
      721 ctttagcgcg gccctatcgc tggagcgctg cgccctgatt tgggcgcgca gcgccttttc
      781 cccggcccgc tcggcggact tgatgcgccg cctctcgttg cgggcggcgt acttggcgtc
      841 cagcttggcc cgccgcttca gggcacgagc ttcggcgcgt cgagtcgctc ggctcttgcg
      901 cttgctgaac aggcccatcc cggctcgcct ccccggtcgt cgtgtcgaca catcgcgatt
      961 ggtggaccca cactatcgcc cgtcgccgat acggccttgc ggtggcgaat gaggccgttc
     1021 taatttcccg gaaggggata tttcgaatgg tacgaatatg cgacaatgaa cgaactctaa
     1081 ggtgcctgcg tgcatcgaga ttgggagttg gggtgaggac atcgtggggc gatcgctaat
     1141 tcgctactcc gcagtcgcct gcgtcgccgc aggcggactg ttcatgggtg gcaccggcgc
     1201 ggtcgcgttc gccgacgacg aaaccggcgg aagcgccaca cagactgccc cgccgacgac
     1261 tgcgtcaccg ccggaccggt tccaactgcc taagtggctg cgattcccaa gcttctcggg
     1321 cacggagccc agggttggct tcatcccgcc gccggtgatg aaactgccgg cgaagctcgg
     1381 gtccaccacg gccccagatg gctcgacgac gctgcaggcc ccgaccatca cgctgccgtt
     1441 cgtcatcggc acggcgacca agccagccgg accgcaatct cctggggcgt tgatcgcccc
     1501 cgacacgggg accgagccag ccggaccgca atctcctggg gcgttgatcg cccccgacac
     1561 ctccgagact cccggcgtgg ttgacggaac ctcgatcgag cagaaccgaa agcccgcggc
     1621 gacgactcaa cagtcgccta tcgacctgac tcggctggtt ccgcccggtg tgcccaccgg
     1681 gccgcccatg acgatcaaga atccgctgcg cgagttgctg cccgccgacc tcgacctgca
     1741 ccagccgctc gtgcgccaac tgcttcctcc gccgctggtg gagatcctga ccgcggtcgc
     1801 gcagcagatt ccgctcgctg gcttggtgat ctcgccggtg atggagttcg tcgttccgcc
     1861 gttcttcgcc gacgtggtga tacccatgct gctgtcggac atcgtcgtgc cgacgctgcc
     1921 gctcgacgcg atattccccg cgccgcttcc tacctcgcta ccgctgacca aggctgcccc
     1981 gttggcactg cccggaagcg gttcgctgcc gccggagttg gccccgatgg gcatggatgt
     2041 gttgcaggcg tcggcatcgc cggcgggccc ggcgtcagaa ccgactgggg tcgagaaact
     2101 gcaagcaccc cttccactac cggacactag cgctgacccg ctggccgagg tggatttccg
     2161 cgccggctac agcgactact tgcgcaacgc gggccaggcg cagatcaccg cgatcgcggt
     2221 gcccggtgcc gtcgcgatct tgctgttcac cgcgggcgga ggcttcatcg gttatcggca
     2281 ggccagggcc ggtcacgtaa tccgagcgga ggggataacc cgcttcctgc gctgacctga
     2341 acagcgtcta cggcaggaca tcgatggtgg aaagggggcg tcatgccggc cagccgacga
     2401 gtcgcccgtg cggtgtctgc cgtcctcaac atggcgcccc gccatggcga agtgtccatc
     2461 gtgcggctgg tgcaggcggt tgcggacagc cgggaccggc gcatcgacat tcagatggag
     2521 gagttgccgc cgggagtgtg tggccagtgg cgccaatacg gtgactacga cgtcttcttg
     2581 atccagcagg ggctacccgc ctgggaccgt accctcgccc acgagctcgg ccacctagtg
     2641 ctcgggcacg aaggcatttc ggtggtggac gccgcccgcg aaggaacgga actggtcagc
     2701 gaggacctca tcaattacat gctgaaccag cgaacgggct gcatgggtcc cgccggggag
     2761 gacgccgaac aggaggcgga ggacttcgcc gcgctgctcg gctaccgcct gggccgactg
     2821 cgctcggacc ggtcgtcgat cgtgcaagtt cgcctcggcg aggcgttcgg gtgattgtct
     2881 gggtcatcgc cggactgctg ggggtggcca ctggacttcg ggtcggttgg gccctggtga
     2941 acaaacaatc ggtggtcagc gccgcgatga tgctcgcgct cggcagcttg ggcttcgtcg
     3001 cggccttgaa ctggcagccg ctcaccctcc tcgttgacac cttgctgcgc tggccgaaca
     3061 tctcggtcgc gctgtctcag atagcgctca cactctgcgc agccgccagt tgcgtgatga
     3121 ttacctccgc tgcgtccgcc aagagacccg tcgtgaccca ccggctggcc tgggccaact
     3181 acggaatcgc ggcggccatc gcgatagcca gcgtcgcgtt gttcttcgtc gacggtcgtc
     3241 aaccagagat ggccccgcag gagtatctgc ggcgcaacct caacgaacac acgtcgttgc
     3301 cttggctgtt gccgctgctg tacatgctgc tggtgttgac ggtcgtgctg tgggccggga
     3361 tgcgctattc caacaccagc cggcgcggcc gcgcgttgtt cgtgttcagc gtcggaatag
     3421 ggctgcttgt attgactctc ggctttttcc tcctgcgcgc cgtcggatcc accgagtttg
     3481 tgggcgtcgg gtcagcgatc acgctgttgg catgcgcgat gctgattgtg gccacggggt
     3541 cgctgttgcc gagtgtcgag gactggttcg gcgctcgccg ggaaatgcgc accattcaac
     3601 cgctgctgcg cgagctctgc ctgcgccagc ccgaggtcgg tgtgggcgtc agaccgagcg
     3661 gcccgttggc cttccaggtg gctgagcaga tgtcgttgat ctccgacgga ctcttcctgg
     3721 aggccactca ggcgcatgat gcggcccgct cgggtcggct ctcaccggct gaccggaaca
     3781 cgccggccgt cagctcacga gagcaagcgc tcgccattgc gagatggatc tacgacagcg
     3841 gccaagacgc cgacaagacg agcggctttc ccgggcttgc gtggctgcgg cagcccgaca
     3901 gctattcaga ccgcgaatgg attctggaga tcgctcggca gtaccacgac ctcatcaccg
     3961 ccggacagtc atccgtgccg gtgggcgaac acgccgaagt tcgcgggacg acgcactagc
     4021 gccgagcctg gctaactgtc tagccgttcc ttgcggcgca gttcctcggc gcgggcgacg
     4081 agatcatcct tggcctccga agataggccg acggtgcgcg ccgcaatgcg ccgcacgccc
     4141 tcgtcgcgca tctccgcgag ccaggtgagc tcctggtcca gctt
//
LOCUS       DASWBF010000516         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_639976,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000516 DASWBF010000000
VERSION     DASWBF010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639976"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..463)
                     /locus_tag="VF874_19100"
     CDS             complement(116..463)
                     /locus_tag="VF874_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX9178083.1"
                     /translation="MGRSNSATLDALITAATADCYNDSECVTGFYTVLDDNLAMPFQT
                     VVLGVEVTVAGIDLTDDEQIMAVCMRGSTAQRIPIVDLALPTPQPDGAEWIAAYRRWV
                     KWRECCGRPPARC"
     gene            complement(632..2173)
                     /locus_tag="VF874_19105"
     CDS             complement(632..2173)
                     /locus_tag="VF874_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9178084.1"
                     /translation="MDIQTDYVVIGTGSAGSVLANRLSADPTVEVAVLEAGPRDKDKF
                     IHIPAAFAKLFRSRFDWDYLTEPQKELDGREIYWPRGKTLGGSSSLNAMMWVRGFAAD
                     YDEWGSLAGDEWDYAHVEKYFTRIENGPLVISRQRSPRSSTAAWLQAVQECGYRVESP
                     NAATPEGFCETIVTQRRGARWSTADAYLKPALSRPNLTLFTEATATRVLFEEARAVGV
                     EFDQDSERRVVRARREVVVCGGAINSPQLLMLSGVGDRERLADHGIDVVTHSPEVGQN
                     LMDHLLVPLGFEALHDTLFAAEKPLQLVNYLARRRGMLTSNVGEAYGFVRSRAELDLP
                     DLELLFGPAPYFDEGIGDPYEGHAVAMGPILLKPRSRGSISLRSADPKDKPVIDPRYL
                     TDDAGADRAALMAGLRMCARIAGSPALRDVVGKIARPIGASKLDDTTLEEALNTLSHT
                     CYHPVGTCRMGRDDASVVDPQLRVRGVEGLRVADASVMPAIIRGHTQAPSVLIGEKAA
                     DLIRS"
     gene            2286..2663
                     /locus_tag="VF874_19110"
     CDS             2286..2663
                     /locus_tag="VF874_19110"
                     /inference="COORDINATES: protein motif:HMM:NF037830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178085.1"
                     /translation="MAKNGPACLGRVEYDGNRIQRITGKTRRIRYLTAAQHISAISDR
                     SIANNLALRIGPATAMSSGAAPAMSTTKRRAQAVADREQITVTAQRGWTVFHKHDDIC
                     RDEVERHTVISTGARLFCVPRAM"
     gene            complement(2775..2905)
                     /locus_tag="VF874_19115"
                     /pseudo
     CDS             complement(2775..2905)
                     /locus_tag="VF874_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884457.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
BASE COUNT          548 a         1031 c          996 g          555 t
ORIGIN      
        1 cgccgaggga cggccggttt tggcgccgca cttgatctcg ccggccgcgc acagctcgag
       61 tacagtgcgc acgttggtga gggtttcagg cgcagttagc cgcacccagt gcccgttaac
      121 atcgtgcagg gggccgtcca caacattcac gccattttac ccagcggcgg tacgctgcga
      181 tccactcagc gccgtcgggc tgcggtgtag gcaacgcaag gtccacgatt gggatgcgct
      241 gtgcggtact accgcgcatg cagacggcca tgatctgttc gtcgtcggtc aggtcaatac
      301 ccgccacggt cacctctacg ccgaggacca cggtctgaaa cggcatggcg agattgtcgt
      361 cgagcacggt atagaagccg gtcacgcact cgctgtcgtt gtagcagtcg gcggtcgccg
      421 ccgtgatcaa agcgtccagc gtcgcgctat tcgatcggcc catccgctca cccccatcgc
      481 ccaccgacca tgtcgtcgca cagactatcg agtgctcgcg cgcctgctgt tcagtacgtc
      541 ctcgacgtgg ggccatcccg gactacgagg tgttccacga atgcctcacc gccggattcg
      601 acgaagtgct cgccctcgcg gcgtgaggcg ctcaggagcg gatcaggtcg gcggctttct
      661 cgccgatcag cacgctgggt gcttgggtgt gcccgcgaat gatggccggc atcaccgacg
      721 cgtcggcgac ccgcagccct tcgacgccac ggacccgtag ctgcggatcg accacgctgg
      781 cgtcatcgcg gcccatccgg caggtgccga ccgggtgata gcaggtgtgc gacagcgtgt
      841 tgagtgcctc ttcgagcgtg gtgtcgtcca gcttgcttgc ccctatcggc cgagcgatct
      901 tcccgaccac gtcacgcaat gccggcgatc ccgcgatcct cgcgcacatt cgcagtcccg
      961 ccatcagcgc cgcgcggtcc gcgccggcgt cgtcggtgag ataccgcggg tcgatgaccg
     1021 gcttgtcctt cgggtcggcc gaccgcagcg agatgctgcc gcggctgcgc ggcttcagca
     1081 ggatcggccc catggccacg gcgtgaccct cataggggtc gccgatgcct tcgtcgaagt
     1141 acggcgccgg cccgaagagc agctcgagat cgggcagatc aagctccgcg cggctgcgga
     1201 cgaagccata cgcctcaccg acgttggacg tcagcatgcc gcgccgccgg gccaggtaat
     1261 tgaccagctg caacggcttt tcggcggcga agagggtgtc gtgcagagcc tcgaagccca
     1321 gcggcaccaa cagatgatcc atcaggttct ggccgacttc gggcgagtga gtcaccacgt
     1381 cgatcccgtg gtcggccagc cgctcgcggt cgccgacgcc ggacagcatc agcagctgcg
     1441 ggctgttgat cgcgccgccg cacaccacca cctcgcggcg ggcgcgcacc acccggcgtt
     1501 cgctatcctg atcgaactcg actccgacgg cacgggcctc ctcgaacagc acgcgggtgg
     1561 cggtggcctc ggtgaacagc gtgaggttcg gccggctcaa cgcgggcttc aggtacgcgt
     1621 cggcggtgct ccaccgcgca cccctgcgct gagtgacaat ggtttcgcaa aagccttccg
     1681 gcgtagccgc attgggcgat tcaacccgat aaccgcactc ctgcaccgcc tgtagccagg
     1741 ccgcggtcga gctgcgtgga ctgcgctggc gcgagatcac cagcggaccg ttctcgatcc
     1801 tggtgaagta cttctcgacg tgcgcgtagt cccattcgtc gccggccagg ctgccccatt
     1861 cgtcgtagtc ggcggcgaag ccacgcaccc acatcatcgc gttcaaggac gacgagccgc
     1921 ccagcgtctt gcctctcggc caatagatct cgcgtccgtc gagctccttc tgcggctcgg
     1981 tcaggtaatc ccaatcgaac cggctgcgga atagcttcgc gaatgccgcg gggatatgga
     2041 tgaacttgtc cttgtcccgg gggccagcct ccagcacggc gacctcgacg gtcgggtcgg
     2101 cgctcaaccg gttggccagg actgatccgg cagagccggt cccgatgacg acgtaatcgg
     2161 tctggatatc cacgcggcgg agtatatgtc gacccacgtg ctcgctgtcg tgagtagtcc
     2221 ggtactcgaa ttcacgtgta ttcccgcaag cggcaggtgc cgggcgatgc cgccgatttg
     2281 gcgtgatggc caagaacggc cccgcatgcc tcgggcgggt ggaatacgac gggaatcgaa
     2341 tacagcggat taccggtaaa acccgccgta ttcgttatct caccgcagcg cagcacattt
     2401 cagctattag cgatcgatcg atcgccaata accttgcgtt gcggataggc ccagctacgg
     2461 cgatgtcttc cggtgccgcg ccggcgatga gcaccacgaa gagacgcgcg caagcggttg
     2521 cggaccgcga acagatcacg gtgaccgcgc aacgcggttg gaccgtgttc cacaagcatg
     2581 acgacatttg ccgcgacgag gttgagcgtc acaccgtgat cagcactggt gcacgcctgt
     2641 tctgcgtgcc gcgcgccatg tgacggcaag caaatcttgc agacgcgaca ccgacaatcg
     2701 tgccgccatc acgggcgatc ggcggtcgct ggccatcggc atggccgggc tgatggcggc
     2761 gcaactcatg aacgcacgtg gagcttgccg gtcagcgaca tcaccgtgat gttgagcccc
     2821 gtgccatgac gatccgcctc agcggataca tggcgctgac gtggcagccc aggaagtaca
     2881 gcggcatctg gggtcccggg gcgtgaatgt agcttcatca gatcgcggta ttgccgacgc
     2941 ggactcgaca ggtccaaggt ccgcgttaaa ggcgcggtca acctggccga cctgtcggat
     3001 gggcattgtc aagttgagcg aagtgggcgc ggtcgccaat cacatcagcc tcatgcgtca
     3061 catatgacct gggggttcac gagaacgcat cgcctcaaat cctggtctga ttgctcaact
     3121 tgacaatgcc
//
LOCUS       DASWBF010000517        14469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_714927,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000517 DASWBF010000000
VERSION     DASWBF010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14469
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714927"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VF874_19120"
     CDS             complement(<1..785)
                     /locus_tag="VF874_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9178086.1"
                     /translation="MPGSSTLAGLLREQLRIRGDHPLLVCDSERIGYAEAGRLSARLA
                     RGLIALGAGKGTHVGLLYPNGVPFVVGMLAAARIGAVVVPFSTFATGPEMHQQLVDSD
                     VEILLAAASYRSHDYTQRFEEMPPAPQLRHRVHDIDRVYRLADTVDETLLSKMEDDVD
                     GSDTLAIVYTSGSTGAPKGVVHTHAALLGHQRNLNEIRGLTAQDKLFCNSPFFWIGGL
                     GFGLLATLVAGSTLVCSNAADAGDTLDLLEAEKPTMTNGFAAS"
     gene            complement(775..2238)
                     /locus_tag="VF874_19125"
     CDS             complement(775..2238)
                     /locus_tag="VF874_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9178087.1"
                     /translation="MMNHPLVQRISGVLELQPDAPAMEFEGQWFSWREIGDTARRIAA
                     LTRPAAQIGILLRNRPAQVAAFLGVLLGGGCVVVINPSRGDHRIRDDLDALRLPMVIG
                     EPDDVAKLESRSTTVVTISGLADEPQLSAADSGVEDGRPGVAVRMLTSGTTGPPKRID
                     LTYDMLSRSVMGAEPDQAPQPTQLRRGVAIVNSPLVHIGGVFRVLQCVAEARAFVLLE
                     RFELRRWAEAVRTHRPGAVSLVPAAVRMVLHSDLTRDDLPGIRAVTSGTAPLSAEDAD
                     AFTEKFGIPVLTSYAATEFGGGVAGWSLADYQKYWQVKRGSVGRATLGAQLRVVDDDG
                     TPLGTDQVGLLEVKPGQLGTGADWIRTTDLARLDDDGFLWILGRADQAIIRGGFKVIP
                     DDVRAALESHPAVQGAAVVGRPDDRLGETPVAMVELRDGASADPYELAEFLQTRLARY
                     EIPTDFAIVDAIPRTPSGKADMSAVRDYFAVPREHAR"
     gene            complement(2235..3017)
                     /locus_tag="VF874_19130"
     CDS             complement(2235..3017)
                     /locus_tag="VF874_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9178088.1"
                     /translation="MTTFETILLDVDAADRVATITLNRPEVLNAFNRTMCDEMAQAWR
                     IVKADESVNAVVLRAAGTRAFSAGLDVKTPYGQPANVWNHEDPGEPLSPKWQKMWKPV
                     VCAVQGMCTAGAFYFINEADVVICSTDATFFDSHVSAGLVCALEPIGLMRRIGLGETL
                     RIALMGNDERVSADTALRIRLVSEVVSSERLWERAHEIAAAIAAKPTSATQGTVKAIW
                     ESLDKPYRAALEQGLIYTRLGNPLGQAELAATEAPATEPRVR"
     gene            complement(3014..3826)
                     /locus_tag="VF874_19135"
     CDS             complement(3014..3826)
                     /locus_tag="VF874_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9178089.1"
                     /translation="MPAKSFDTIMYEVDGHTATITLNRPDALNALSPHMITELRAAYQ
                     QAENDDNVWLLIVTGTGRAFCTGADVKEIPGDGKVINERPYLSTYEQWEAQQEGTPPF
                     RTMAKPVLAAINGLCCGAGVDWVTTSDIVIASEKATFFDPHVSIGLVAGREVVRLAGV
                     LPRSVALRMALMGKHERMSAQRALELGMISEVVEHERLLERAHEIADIVNSNAPLAVR
                     GTRLAILKGLDLPLHEAEMLAEAFRERNLHTEDSLEGPKAFVEKRTPDWQCR"
     gene            complement(3841..4656)
                     /locus_tag="VF874_19140"
     CDS             complement(3841..4656)
                     /locus_tag="VF874_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9178090.1"
                     /translation="MWRLAVAGDGSVATQRDGAVLRITLDRPDRRNALSHEMVDAFVA
                     ALTNAATDDSLRAIHLTGEGADFCAGADWVSTNEAGGPHPRTGDLVRRIPHTTHRVIE
                     LIHTVHLPVVCSVRGYAVGLGCSLALAADFAVAATGAVFWEPFLARGFTPDSGSTWFL
                     PRLVGLARAKQMLLLGEKVPAAEAADWGLIHRSVDDSELDDAAEHLLTRLAAGPTVAL
                     GLAKQAINYAQHATLTQAMTQELFNLELSCRTGDFKEGLAAFRQRRAPDFQGR"
     gene            4732..5388
                     /locus_tag="VF874_19145"
     CDS             4732..5388
                     /locus_tag="VF874_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178091.1"
                     /translation="MARENDDRRFGEQPLAQTVAAAAAIRRLTGMLLSLEHPHPTVDA
                     MLAQFADWERELADVVPPDSAPRRDSDGDDKRVYLGHAFDIGAFNPCFPEYRFDHLDA
                     ETASGTVTFPLVFEGPPGLVHGGFLAVFFDCVTQHHKCAAARTGRTRSLTVTFRRPTP
                     VLTELRFDIVRSEVDGAVTSTARILHDDQVLCIGELNALAMAPEQLAVSRYGGRREIG
                     "
     gene            5385..6191
                     /locus_tag="VF874_19150"
     CDS             5385..6191
                     /locus_tag="VF874_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9178092.1"
                     /translation="MTRPALQRIRQPRVAEIVASQLRDDILSGRLKEGDVLPSQETLF
                     HEFGVSPPALREAIHILEVDGLVSVRRGNVGGALVHLPSAERTAHMISMVLQARAATP
                     ADVSGALLYLEPICAGMCAARPDRITEVVPLLVAEIERQTAQFDDLSQYVPNARRFHE
                     AIVSRCGNEPMILLIGSLELIWSAHESSVWSDDLPTDERLREEQRTMTAKTMRAALRD
                     HQRLLDAIRDGQQDRAIRLAQDHLAVARSNTLAFGRDKTIEAKRISDNGR"
     gene            6243..7154
                     /locus_tag="VF874_19155"
     CDS             6243..7154
                     /locus_tag="VF874_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9178093.1"
                     /translation="MTTSDDRVLFDMEPEQRIATITLNSPKQRNSYDATMRDSIARCL
                     DRVAEDDDLTVVLLRGADGVFSTGADMNNAYGWYGDERLREERRATAKGGGEKTAKSR
                     PSQRRRLTVDRKSFSFYHNLMGFPKVTVGEISGYALGGGFEIALMTDISVIARDTKIG
                     MPATRFLGPALGSLHMFFHRLGPVLARRLLLTGDIIEAGTIEHLGIFTETCTAEHVGA
                     RARYWAEKAAKMPADGVVIAKEAFRLVEQSQAYQGEEVASYLFHAFGTNLQFAPGEFN
                     FVKTRAQVGTKEAFRLRDEHFHVPEPE"
     gene            7230..8405
                     /locus_tag="VF874_19160"
     CDS             7230..8405
                     /locus_tag="VF874_19160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9178094.1"
                     /translation="MPTPVIVGATRTAIGRSFKGTLANTPPETLATTVLPEVVRRAGL
                     DPAAIDDIIFAESHYGGGDLARYAAAACGMEDVPGQSVNRHCAGSLTAIGNAAAGIGS
                     GMERVVIAGGVQSLSMMPLMKWRVPGPELTFEEPWMPPTHVETPDAPTRDMSITVGWN
                     TAQAVGITREEMDAWAARSHQRAIAAIDAGKFVDEIVPLKVTLPDGSVVDFSVDEYPR
                     RDTTVEKLAGPKVLHPEIEGFSITAGNSSGTNDAAAAVALVEKDYAAAENLTVLGTVK
                     AWAASGVAPRDTGLGAVKVIGKVLQRAGLTPADVALWEINEAFASVPIAACREYGLDE
                     ERVNFSGSGCSLGHPIAASGARMVTTLVYELQRRGGGIGVAAMCAGGGQGGAVVIEV"
     gene            complement(8410..9153)
                     /locus_tag="VF874_19165"
     CDS             complement(8410..9153)
                     /locus_tag="VF874_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX9178095.1"
                     /translation="MPSKATAAKRQRRERGSINPEDIIDGAFQLADEVSIDNLSMPLL
                     GKRLGVGVTSIYWYFRKKDDLLNAMTDRALSKFTFETPYVEASDWRESLRNHARSMRK
                     TFLSSPILTDLILIRGALSAKARRVGVEQMEKAIETLVGAGLSPEDAFDTYSAVSLHV
                     RGTVVLQRLQEKTDELDDGERAGFEPRTIDPKITPLIAKLAAKGHRLAAADEKNFEYG
                     LDCILDHASRLIEEGQKAPRRARTTAAKR"
     gene            9403..10200
                     /locus_tag="VF874_19170"
     CDS             9403..10200
                     /locus_tag="VF874_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9178096.1"
                     /translation="MDLGLKDAKAVVVGGGRGMGLAAARCLADDGCRVALVGRSRAVL
                     EQAADALAGRGSPDAVGLVADVRDVAGVEKLFGELAERWNSELNVLINAVGPNAIGTF
                     DDLTDDQWREAVDDGVMGMVHCVRAALPLLRRAEWARIVNFSAHSTQRQSVILPAYTA
                     AKAMVNSISKNLSLLLAKDEILVNVVSPGSIASESLVGWASSVGVDGSDPYALMDAIA
                     EHFGHPAHLPRAGLPDEIGPVVAFLASRRNSYMTGANVNVDGGSDFT"
     gene            10202..11209
                     /locus_tag="VF874_19175"
     CDS             10202..11209
                     /locus_tag="VF874_19175"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9178097.1"
                     /translation="MATASEAREWAHHALRGIGDSLYTPFRGADGDDIDWDAYAALVR
                     YCVGDLAHPMLWLTSGVGEFWSLTIAERKRLLEVAIDEARAINPDVIVQSCTAALSAK
                     DCLELTQHAQESGADIVYIQTPMMELHAGEGVLKFFRYISDRTDIALGMFNSPSSGYV
                     LNAAESARVVEEIPAVCATKEGAFRPANSRLLHELAPDLVIWECDTTVYRAGWLREGI
                     VGPAQLGTICYLYETPQRRILSEYWEMIWNDRLVEAMDYAVESGLDQFGVDMRSWFTC
                     YPLRPDYFTHWGGAFKYAASVLGLPVGDYPHSRPPQAELPEQARVQIRDAYRRFGLVD
                     Q"
     gene            11289..12164
                     /locus_tag="VF874_19180"
     CDS             11289..12164
                     /locus_tag="VF874_19180"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9178098.1"
                     /translation="MSVPDPAVAADHAVLYEVCDSGVAVLTLNRPDRMNAWGGGLATA
                     FWSCLDRAEAAPDVRAVVVTGSGRAFCAGADMGDLSTISNTTMDSAGDTDAGAAAPAT
                     LDIDKLVGERHPYVVATLRKPVIAAINGACAGIGLTQALMCDVRFAAEGAKFTTSFAR
                     RGLIAEYGISWILPRVVGWGAALDLLLSGRVFYADEAAALGLVKEVVPPEELLPRAIA
                     YAEDIAANCAPNSLAVIKQQVYADTMRDVFEASAVAEKLMHESMLRPDFIEGITSFFE
                     KRAPHFPPLSEVRDD"
     gene            12192..13391
                     /locus_tag="VF874_19185"
     CDS             12192..13391
                     /locus_tag="VF874_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9178099.1"
                     /translation="MALDYKAIDVDNHYYEPLDAFTRHLDKMFKRRGVQMVSDGKRTL
                     SIIGDRVNRFIPNPTFDPIIVPGCLDLLFRGEIPDGVDPASLMKVERLAEHPEYQNRD
                     ARVEVMDTQNIETVFMLPTFACGVEEALKHDIDATMASVHAFNLWLDDDWGFDRPDHR
                     IIAAPIISLADPERAVEEVDFVLSRGAKLTLVRPAPVPGVVKPRSLGDPVHDPVWARL
                     AEAGVPVGFHLSDSGYLQVAAMWGGKSTFEAFGGSNPLDQILVDDRAIHDTMASMIAH
                     GVFTRHPKLRAASIENGSYFVYRLIKRLKKVANNHPRLFPEDPVEQLRNNVWIAPYYE
                     DDLAQLAEVIGVDKILFGSDWPHGEGLESPVSFTEELKGFSDADIRKVMRDNALDLLG
                     VKVGSAA"
     gene            13400..>14469
                     /locus_tag="VF874_19190"
     CDS             13400..>14469
                     /locus_tag="VF874_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9178100.1"
                     /translation="MGEWTIGAVLDAIADVIPDRTMTVCGNRRSTYAESADRTRRLAN
                     FLAAKGFGAHRERDALERWECGQDRIALIIHNDLYPDMVIGCLKARTVPVNVNYNYAS
                     REIGELLEYIQPRAVIYHRSLGAKFADVLPPDSADLLISIDDGSTAPELPGAVTLDDA
                     LAQGDTDQHSSPSPDDLIMICTGGTTGRPKGVLWRQSDIYVSGMVGADHEAVDEIHGK
                     VRQTGPPWFAVSPLMHAAGMWTAFSAIMARAPVILYDTCKKLDPRLVWETAEREKVGL
                     MTMVGDAYAAPLVEELQRGTYDLSSLYAIGTGGAATNPKYQRALLESLPQLTLINGYG
                     SSETGNMGFGHSQQGNQTDTFQL"
BASE COUNT         2522 a         4680 c         4787 g         2480 t
ORIGIN      
        1 atcgaggcgg cgaaaccgtt ggtcatcgtt ggcttctcgg cttccagcag atcaagagtg
       61 tcgccggcgt cggcagcgtt cgagcacacc agtgtcgagc cggcgaccag cgtggcgagc
      121 agaccgaacc ccaggccgcc gatccagaag aacggcgaat tgcagaagag tttgtcttgc
      181 gctgtcagcc cgcggatctc gttgaggttg cgctgatgtc ccagcagcgc tgcgtgggtg
      241 tggacaacgc cttttggggc gccggtggaa ccggacgtgt acacgattgc cagcgtgtcc
      301 gaaccatcga cgtcgtcttc catcttcgaa agcagcgtct cgtcgacggt gtccgcgagg
      361 cggtaaacgc ggtcgatgtc gtgaacgcgg tgacgtagct gaggtgccgg cggcatctct
      421 tcgaaccgct gcgtgtagtc gtgggagcgg tacgacgctg cggcaagcag gatctcgaca
      481 tcgctgtcga ccagttgttg gtgcatttcc ggcccggtcg cgaacgtgga gaacgggacg
      541 acgacggcgc cgatgcgcgc ggccgccagc atgccgacca cgaacggcac accgttgggg
      601 tagagcagcc cgacatgcgt gcccttgccc gcgccgagcg cgatcaagcc acgggccaac
      661 cgtgccgaga gacggccggc ctctgcgtag ccgatgcgtt cgctgtcgca caccagcagc
      721 ggatggtcgc ctcggatccg caactgctcg cgcagaagcc cggcaagcgt ggaactaccg
      781 ggcatgctcg cgggggacgg cgaaatagtc tctgacggca ctcatatcgg ccttacccga
      841 cggtgtcctc ggaatcgcgt ccacaatcgc gaagtcggtc ggaatctcgt agcgcgccag
      901 ccgcgtttgg aggaactcgg caagctcata gggatcggcg gacgcgccgt cgcgaagttc
      961 caccatggcg accggcgtct caccgagacg gtcgtctggt cgcccgacga ccgccgcgcc
     1021 ttgcaccgcc ggatggctct ccaacgcggc gcgcacgtca tccggtatca ccttgaatcc
     1081 gccgcggatg atcgcctggt ctgcgcgccc gagaatccac aggaagccgt catcgtcgag
     1141 ccgggccaga tcggtggtcc ggatccagtc cgccccggtg cccagctggc cgggtttgac
     1201 ctccagcagt ccgacctggt cggtccctaa cggtgtgccg tcgtcgtcga ctacccgcag
     1261 ttgcgcaccc agcgtcgcgc ggcccacgct gcctcgtttg acttgccagt acttctggta
     1321 gtcggccagt gaccagccgg ccacaccgcc accgaattcc gttgccgcat aggatgtcaa
     1381 gaccggaatg ccgaacttct cggtgaacgc gtcggcatct tcggccgata gcggcgcggt
     1441 gccggacgtg acagcgcgga tgcctggcag gtcgtcgcgc gtgaggtcgg agtgcagcac
     1501 catccgcacc gccgcgggca ccagcgacac ggcacccggc cggtgcgtgc gcaccgcctc
     1561 ggcccatcgg cgcaactcga atcgctccag gagcacgaag gccctcgcct cggcaacgca
     1621 ctgcagcacc cgaaacaccc cgccgatgtg cacgagtggc gagttgacga tcgcgacgcc
     1681 gcggcgcagc tgggtcggct gcggtgcctg gtccggttcg gcgcccatga cgctgcggga
     1741 cagcatgtcg tacgtgaggt cgattcgctt gggcggcccc gtcgttccgc tggtcaacat
     1801 ccggaccgcg acgccggggc ggccatcttc gacaccggag tcggccgccg acaactgcgg
     1861 ctcgtccgcc agtcccgaga tcgtcacgac cgtggtactt cgtgactcga gtttcgcaac
     1921 gtcgtcgggt tcaccgatga ccatcggtag ccgaagggcg tccagatcgt ctcggatgcg
     1981 gtggtcgccg cgcgacgggt tgatcacgac gacacatccg ccgcccagca gcaccccgag
     2041 gaacgcggcc acctgcgcgg gtcgattccg cagcagtatg ccgatctgcg cagcggggcg
     2101 cgtcagggca gcgatccggc gagcggtgtc gccgatttcg cgccacgaaa accactggcc
     2161 ctcgaactcc atcgccggcg cgtcgggctg caactccagt acgccggaga tacgctgaac
     2221 cagcggatga ttcatcaccg aaccctcggc tcagtcgcgg gcgcttcggt tgcggcgagt
     2281 tcggcctgtc cgagcgggtt acctagccgc gtgtagatca gtccctgttc cagcgcggca
     2341 cggtagggct tgtccagcga ctcccagatc gccttcaccg taccctgggt cgctgaggtc
     2401 ggcttggccg caatcgccgc cgcgatctca tgtgcgcgtt cccaaagccg ttcggacgac
     2461 accacttccg acaccagtcg aatccgcagg gcggtgtctg cgctgacccg ctcgtcgttg
     2521 cccatgagcg cgattcgcag cgtctcaccc aagccgatgc gtcgcatcaa cccgatcggc
     2581 tccagcgcgc agaccagccc ggcactgacg tgcgagtcga agaacgtcgc gtcggtagag
     2641 cagatcacca cgtccgcttc gttgatgaag tagaacgcac ccgcggtgca catgccctgg
     2701 accgcgcaca ccaccggttt ccacatcttc tgccacttgg gactgagcgg ttcaccggga
     2761 tcctcgtggt tccacacgtt ggccggctgc ccatagggcg tcttgacgtc cagcccggcg
     2821 ctgaacgccc gtgtgccggc tgctcgcaac acgacggcgt tgacggactc gtcggctttg
     2881 acgatgcgcc acgcctgcgc catctcgtcg cacatcgtcc ggttgaacgc gttgagcacc
     2941 tcgggccggt tcaacgtgat ggtcgcgacg cggtctgccg cgtcgacgtc gagcaggatc
     3001 gtctcgaagg tggtcatcga cactgccagt ccggagtccg cttctccacg aacgctttgg
     3061 gcccttcgag tgagtcctcg gtgtgcaggt tgcgttcgcg gaaggcttcg gcgagcatct
     3121 cggcttcgtg cagcggaaga tccaggccct tgaggatcgc cagccgggtg ccccgcaccg
     3181 ccagcggcgc gttcgagttg acgatgtcgg cgatctcatg ggcgcgttca agaagacgct
     3241 cgtgctcgac gacctcgctg atcatcccga gctccaacgc ccgctgcgcg ctcatccgtt
     3301 cgtgcttgcc catcaacgcc attcgcagcg cgaccgaccg cggcaacacg ccggccagtc
     3361 gcaccacttc gcgcccggcc accaacccga tgctgacgtg aggatcgaag aatgtcgcct
     3421 tctcggacgc gatcacgatg tctgaagtgg tgacccagtc gacgcccgcg ccgcagcaca
     3481 ggccgttgat ggcggccagc accggtttgg ccatggtccg gaacggcggc gtgccctcct
     3541 gctgtgcctc ccactgctcg taggtggaca ggtacggccg ctcgttgatg accttgccgt
     3601 cgccgggaat ctccttgacg tcagcgccgg tgcagaacgc gcgcccggtg ccggtgacga
     3661 tgagcagcca gacgttgtcg tcgttttcag cctgctgata cgccgcgcgc aactcggtga
     3721 tcatgtgcgg gctcagcgcg ttgagtgcgt cgggccgatt cagcgtgatc gtggccgtgt
     3781 gcccgtcgac ttcatacatg atggtgtcga acgacttggc tggcatgagt gtcccttcgg
     3841 tcagcggcct tggaagtccg gtgctcgccg ttgccggaac gccgccaggc cttctttgaa
     3901 gtcgcccgtg cggcaggata attcgaggtt gaacagttcc tgggtcatcg cctgcgtcag
     3961 ggtcgcatgt tgggcgtagt tgattgcctg cttggccaag ccgagcgcca ccgtcggccc
     4021 cgccgccagc cgcgtgagga ggtgctccgc agcgtcgtcg agttctgagt cgtcgacgct
     4081 gcggtggatc aacccccagt cggcggcctc ggcggcaggc accttctcgc cgagcagcag
     4141 catctgtttc gcccgggcca gtccgacaag ccggggcagg aaccatgtgg acccggaatc
     4201 gggggtgaag ccgcgcgcca gaaagggttc ccagaagacg gcgccggttg cggccaccgc
     4261 gaagtcggcg gccagggcga gactgcatcc gaggccgacg gcgtagccgc gcacgctgca
     4321 tacgacgggc agatgaacgg tatgaattag ttcgatcacc cggtgcgtag tgtgcggaat
     4381 gcggcggacg aggtcacccg ttcggggatg cggtccgccg gcctcgttgg tggacaccca
     4441 gtctgccccg gcgcagaagt cggcgccctc gcccgtgagg tgaatcgcgc gaagcgaatc
     4501 gtcggtggcg gcgttggtca gcgcagcgac gaacgcgtcc accatctcgt ggctcaacgc
     4561 gttgcgccga tccgggcgat ccagcgtgat ccgcagcacc gcaccgtcgc gctgcgttgc
     4621 caccgacccg tcgcccgcca ccgccaacct ccagatattt acggttgccc atacagtagg
     4681 ctactacagt attatcttgt ataagttagc aaggaaaggg ctcccagcgg aatggcccgt
     4741 gagaacgacg accgccggtt cggtgagcag ccgctggccc agacggtcgc tgcggctgcc
     4801 gcgatccgcc gcctgacggg catgctgctc tcacttgaac atccccaccc gaccgtcgac
     4861 gcgatgctcg cgcagttcgc ggactgggaa cgcgaactcg ccgacgtcgt tccgccggac
     4921 agtgcccctc ggcgcgactc cgacggcgac gacaagcgcg tctacctcgg ccacgcgttc
     4981 gacatcggcg cgttcaaccc gtgcttcccg gaataccgct tcgaccatct cgacgccgag
     5041 acggcatccg gaaccgtcac ctttcccctc gtcttcgagg ggccgccggg cttggtccac
     5101 ggtggcttcc tcgccgtgtt cttcgattgc gttacccagc accacaagtg cgcggccgcg
     5161 cgcaccggca ggacccgctc gctgaccgtg acgtttcggc ggccgacgcc ggtgctgacc
     5221 gaactgcgct tcgacatcgt ccgatccgag gtggacggtg cggtcacgtc gaccgcgcgg
     5281 atcctgcacg acgatcaggt gctctgcatc ggcgaattga atgcgttggc aatggctcca
     5341 gagcaactgg ccgtgtcccg atacggcggg cgacgggaga tcggatgacc cgcccggcgc
     5401 tgcagcggat ccgccaaccg cgggtcgccg aaatcgtcgc atcgcaactg cgcgacgaca
     5461 tcctgtcggg acgtttgaag gaaggggacg tgctgccgtc acaggagacg ctgttccacg
     5521 agttcggcgt aagtcctcca gcgctgcgcg aagccataca catcctggaa gtcgacggcc
     5581 tggtctcggt gcggcggggc aacgtcggcg gagcgctcgt ccaccttccg tcggccgagc
     5641 ggaccgccca catgatcagc atggtgctgc aggcgcgggc tgcgacaccg gccgacgtca
     5701 gcggcgcgct gctttatctc gagccgatct gcgccgggat gtgcgccgcc cgcccggacc
     5761 ggataaccga ggtcgtaccg ttgttggtag ccgaaatcga acggcagaca gcgcaattcg
     5821 atgacctgtc gcagtacgtg ccaaacgctc ggcgcttcca cgaggcgatc gtctcccgtt
     5881 gcggcaacga gccgatgatc ctgctgatcg gctcgctcga gttgatctgg tcggcgcacg
     5941 agtcgtcggt gtggagcgac gacttgccga ccgatgagcg cttgcgcgaa gagcaacgga
     6001 ccatgacggc caagacgatg cgtgccgcgc tgcgcgacca tcaacggctg ctcgacgcga
     6061 tccgcgacgg gcagcaagac cgggcgattc gcctcgcgca ggaccacctc gccgtggcgc
     6121 gcagcaacac gctcgcgttc ggcagagaca aaaccattga ggccaagcgg atctcggaca
     6181 acggccgatg agccgcttgc gcgaagagga aacaggcaca gccctttaga aaggtccggc
     6241 aaatgacgac atcagatgac cgtgttctgt tcgacatgga accggaacag cgaatcgcca
     6301 cgatcacgtt gaacagcccg aagcaacgca attcctacga cgccacaatg cgagatagca
     6361 tcgcgcgctg cctggatcgg gtcgccgagg acgacgatct caccgtcgtg ctgctgcgcg
     6421 gagccgacgg agtgttctcc accggcgctg acatgaacaa cgcctacggc tggtacggcg
     6481 atgagcgctt gcgcgaagag cggagagcga ccgcgaaggg tggcggcgag aagaccgcga
     6541 aaagccgccc cagccaacgc cgccgactca cggtggaccg caagtcgttc agcttctacc
     6601 acaacctgat gggcttcccg aaggtcacgg tcggggagat cagcggctac gcgctcggcg
     6661 gcggtttcga gatcgcgctg atgaccgaca tctcggtgat cgcgcgcgac acgaagatcg
     6721 gcatgccggc gacgcgcttc ctcggacccg ctcttggcag cctgcacatg ttcttccacc
     6781 ggctcggacc ggtgctggcc cgacgacttc ttctcaccgg cgacatcatc gaagccggca
     6841 cgatcgagca tctcggcatc ttcaccgaga cgtgcaccgc cgagcacgtc ggcgcccggg
     6901 cacggtactg ggcggagaag gccgcgaaaa tgcccgccga cggcgtcgtc atcgccaagg
     6961 aggcgttccg cctcgtcgaa cagagccagg cctatcaggg cgaggaggtg gccagttatc
     7021 tgttccacgc cttcggcacg aacctgcaat tcgcgccggg cgagttcaac ttcgtcaaga
     7081 cgcgggcgca ggtcggcacc aaggaggcct tccggctgcg cgacgagcac ttccacgtgc
     7141 cggagccgga gtgacgactg ccacctactg atagcgctac agtatctgct atttttgtaa
     7201 agtcgttttc accgaacccg aggtagacca tgcccacacc tgtcatcgtc ggcgcaacca
     7261 ggacagcgat cggacgatcg ttcaagggaa cgctggccaa cacgccacca gaaacgctgg
     7321 ccaccacggt gctgcctgag gtggtccgcc gggccggtct cgatcccgcc gccatcgacg
     7381 acatcatctt cgccgaatcc cattacggtg gtggcgatct cgcccgctat gcggccgcgg
     7441 catgtggcat ggaagacgtt cccggtcagt cggtgaatcg gcactgcgcg ggaagtttga
     7501 ccgcgatcgg caacgcggcc gccggaatcg gctccggcat ggagcgcgtc gtgatcgccg
     7561 ggggagtgca gtcgctgtcg atgatgccgc tgatgaagtg gcgggtgccc ggcccagagt
     7621 tgaccttcga agaaccgtgg atgcctccga cgcacgtgga aacccccgat gcgccgacgc
     7681 gtgacatgtc gatcaccgtg ggctggaata ccgcccaggc agtcggcatc acgcgcgagg
     7741 agatggacgc gtgggcggcg cgatctcatc agcgcgcgat cgccgcgatc gacgcgggca
     7801 agttcgtcga cgagatcgtg ccgctgaaag tgacactgcc cgacggctcg gtggtcgact
     7861 tctccgtcga cgagtatccc cgtcgcgaca ccacggtcga gaagctggcc ggaccgaagg
     7921 tgctacaccc ggagatcgaa ggtttctcga tcaccgcggg caacagcagc ggaaccaacg
     7981 acgccgccgc ggcggtggcg ctcgtcgaga aggactacgc cgcggccgag aacctgaccg
     8041 ttttgggcac cgtgaaggcc tgggcggctt ccggtgtggc accgcgggat accgggctcg
     8101 gagcggtcaa ggtgatcggc aaggtgctgc agcgtgccgg attgacgccc gccgatgtcg
     8161 cgctgtggga gatcaacgag gcgttcgcgt cggtgccgat cgcggcgtgc cgcgagtacg
     8221 ggctcgacga ggagcgggtg aacttctccg gcagcggatg cagcctcggc catcccatcg
     8281 ccgcgtccgg cgcgcgcatg gtgaccacgc tggtttacga actgcagcgg cgcggcggcg
     8341 gcatcggcgt cgccgcgatg tgcgcgggcg gcggccaagg cggcgccgtg gtcatcgagg
     8401 tctgacgggt cagcgtttcg cggcggtcgt gcgcgcgcgg cgcggcgcct tctgtccctc
     8461 ttcgatcagc cggctcgcat ggtcgaggat gcagtcgagc ccgtactcga aattcttctc
     8521 gtccgcggcg gcgagccggt gccccttggc ggccaacttc gcgatcaatg gcgtgatctt
     8581 cggatcgatc gtcctcggtt cgaagccggc acgctcaccg tcgtccaact cgtccgtctt
     8641 ctcctgcagg cgctgcagga ctactgtgcc gcggacgtga agcgacaccg cagagtaggt
     8701 gtcgaacgcg tcctcgggtg aaagtcctgc tcccaccaac gtttcgatgg ctttctccat
     8761 ctgctcgaca ccgacccgcc gcgccttcgc gctgagggcc ccccggatca gaatcaagtc
     8821 agtgaggatc gggctgctca aaaatgtctt gcgcatcgag cgggcgtggt tgcgcaacga
     8881 ctcgcgccag tcactggcct cgacataggg cgtctcgaac gtgaacttgc tgagagcgcg
     8941 gtcggtcatc gcattgagga gatcgtcctt cttgcggaag taccagtaga tgctcgtcac
     9001 acccaccccg agacgcttgc ccagtagggg catgctcagg ttgtcgatgg agacctcgtc
     9061 ggcgagctgg aatgcgccgt cgatgatgtc ctccggattg atcgaaccgc gttcgcgccg
     9121 ctggcgcttg gcagcggttg ccttgcttgg cacggtgggt cacctccatc ggactagtca
     9181 ccgttaacaa taccggtagc gatccgattc ctgtctcggg aaagctttcg gtatctgcgg
     9241 tgtctggtgt gcggggcacc ggcgactgtg cggtttcgta cgcgacacgc cgtgtccggt
     9301 ggacaaattc gcacactcac ctgtcttcga acgtccgcga gcacggtagg aaaccgcaca
     9361 atcgcagcgc cgcccggtca acgcgattta ggctgtaaag ctatggatct cgggttgaaa
     9421 gacgcgaaag cggtggtggt cggcggtggc cgcgggatgg ggctggccgc cgcgcgctgc
     9481 ctggccgacg acggctgcag ggttgccctc gtcggccgct cacgagccgt cctggagcag
     9541 gcggccgatg ccttggccgg tcgcggcagc cccgacgccg ttggtctggt ggccgacgtc
     9601 cgagacgtcg caggcgtcga gaagctcttc ggcgaactcg ccgagaggtg gaacagcgag
     9661 ctgaatgtgc tcatcaatgc agtcggaccg aacgcgatcg gcaccttcga cgacctgacc
     9721 gacgaccaat ggcgggaagc cgtcgacgac ggcgtgatgg ggatggtgca ctgcgtccgg
     9781 gcggcgctgc cgttgcttcg gcgggccgag tgggcgcgga tcgtcaactt ctcggcgcat
     9841 tcgacgcagc ggcagagcgt gattctgccc gcatacaccg ccgccaaggc gatggtgaac
     9901 agcatctcga agaacctgtc gctgcttctg gccaaggatg agattctggt caacgtggtg
     9961 tcaccaggca gcatcgcgtc cgagtcgctc gtcgggtggg cctcgtccgt gggtgtcgac
    10021 ggctctgatc cctatgcgct aatggacgcg atcgccgagc atttcggcca tccggctcat
    10081 ctgccgcgcg ccggcctccc cgacgagatc gggcccgtcg tagcgttttt ggcgtcccgc
    10141 cgcaactcgt acatgaccgg tgcgaacgtc aacgtcgacg gcggatcgga cttcacctga
    10201 gatggcaacg gcaagcgaag cacgggagtg ggcgcaccac gcgcttcggg gtatcggcga
    10261 ttcgctgtac acaccgtttc gcggagcaga cggcgacgac atcgactggg acgcctatgc
    10321 cgcgctcgtg cgctactgcg taggtgatct cgcccacccg atgctctggc tgacaagcgg
    10381 tgtcggcgag ttctggtcgt tgacgatcgc cgagcgaaag cgcttgctgg aggtcgcaat
    10441 cgacgaagcg cgcgcgatca accccgatgt catcgtgcag tcgtgcacgg cagcgctttc
    10501 ggcgaaggat tgcctcgaat tgacgcagca cgcccaggaa tcgggcgccg acatcgtcta
    10561 catccagacg ccgatgatgg agctgcacgc cggtgagggg gtgctgaagt tcttccgcta
    10621 tatcagcgat cgcacggaca tcgcgctggg gatgttcaat tcgccgtcgt cgggctacgt
    10681 cctgaacgcc gccgagagcg cacgcgtcgt cgaggagatt ccggcggtct gcgcgacgaa
    10741 ggagggcgcg tttcggccgg cgaacagccg cctgctgcac gagctagcgc ccgacctggt
    10801 gatctgggaa tgcgacacga cggtgtaccg cgcggggtgg ttgcgcgaag ggatcgtcgg
    10861 gccggcgcaa ttgggcacca tctgctatct ctacgaaacg ccgcagcggc ggattctcag
    10921 tgagtactgg gagatgatct ggaacgacag actggtcgag gcgatggact acgccgtcga
    10981 gtcgggcctg gatcagttcg gcgtcgacat gcggtcgtgg ttcacctgct atccactccg
    11041 tccggactac ttcacgcact ggggcggcgc gttcaagtac gcggcgtcgg tgctcggttt
    11101 gcccgtcggc gattacccac attcgcgacc gccacaagcc gaactccccg agcaggcgcg
    11161 ggtgcagatc cgggacgcgt atcggcgctt cgggctggtc gatcagtgag gcgccgagcg
    11221 attgcggaag gcgctaccga taggtgtaca gtatgtgcat acatgccacg cctgaggagg
    11281 tcagccgggt gagcgttccc gaccccgcgg tcgcggctga ccacgcggtt ttgtacgagg
    11341 tgtgcgacag cggggtggct gtgctgacgt tgaaccggcc cgaccgcatg aacgcgtggg
    11401 gcggcgggct ggcgaccgcg ttctggtcat gcctcgaccg agccgaagcc gcccccgatg
    11461 ttcgtgctgt cgtcgtcacc ggaagcggac gggcgttctg cgccggcgcc gacatggggg
    11521 acctgtcgac gatcagcaac accaccatgg atagcgccgg cgacaccgac gccggagccg
    11581 cagcgccggc gacattggac atcgacaagc tggtgggaga acgtcatccg tatgtcgtgg
    11641 cgacactccg caagccggtc atcgcggcga tcaacggcgc gtgcgcgggc atcggcctga
    11701 cccaggcgtt gatgtgcgat gtccggttcg ccgcggaagg cgccaagttc accacgtcgt
    11761 ttgcccggcg cgggctgatc gccgaatacg gaatctcctg gatcctcccg cgcgtcgtgg
    11821 ggtggggtgc cgcgctcgat ctcctgctga gcggccgggt gttctacgcc gacgaagcgg
    11881 cggcgctcgg actggtcaag gaagtggtac cgcccgagga actgctgccg cgcgcgatcg
    11941 cctacgccga ggatatcgcc gcgaactgcg cgccgaattc cctggcggtg atcaagcagc
    12001 aggtgtacgc agacactatg cgcgacgtct tcgaggcgag tgccgtggcg gagaagctga
    12061 tgcacgagtc gatgcttcgc cccgacttca tcgagggcat cacgagcttc ttcgagaagc
    12121 gtgcacccca ctttccgcct ctgagcgagg ttcgcgacga ctagcacagg ccgactgaaa
    12181 ggacagaact catggcgctg gactacaagg ccatcgacgt cgacaaccac tactacgagc
    12241 cgctggacgc gttcacccga catctggaca agatgttcaa gcgtcgcggt gtgcagatgg
    12301 tcagcgatgg caagcgcacg ctgtcgatca tcggggaccg agtcaaccgc ttcatcccca
    12361 acccgacgtt cgacccgatc atcgtgccgg gatgcctgga tctgctgttc cggggcgaaa
    12421 tccccgacgg cgtcgacccg gcgtcgctga tgaaagtcga gcgcctcgcg gagcacccgg
    12481 agtatcagaa ccgcgacgcc cgcgtagagg tgatggacac ccaaaacatc gaaaccgtgt
    12541 tcatgttgcc gacattcgcg tgcggcgtcg aggaagcgct caagcacgac atcgacgcga
    12601 cgatggcgtc agtgcatgca ttcaacctgt ggctcgacga cgactggggg ttcgatcggc
    12661 ccgatcatcg gatcatcgca gcaccgatca tctcgctggc tgatcctgaa agagccgtcg
    12721 aggaagtcga tttcgtgttg tcgcgtggcg ctaagctgac tcttgtgcgg cccgccccgg
    12781 tgccaggtgt cgtcaagccg cgttcgttgg gcgatccggt ccatgacccg gtctgggccc
    12841 gactggccga ggccggagtt ccggtgggat tccacttgag tgacagcggc tacctgcaag
    12901 tggcggcgat gtggggcggc aagtcgacgt tcgaggcgtt cggcggctcg aatccgttgg
    12961 accagatact cgtcgacgac cgcgcgatcc acgacacaat ggcctcgatg atcgcgcacg
    13021 gcgtgttcac ccgtcatccg aaactgcggg cggcgagcat cgagaacggt tcgtacttcg
    13081 tgtaccgctt gatcaagcgg ctgaagaagg tggccaacaa ccatccgcgc ctgtttcccg
    13141 aggatccggt ggagcagttg cgcaacaacg tgtggatcgc cccgtactac gaggatgatt
    13201 tggcccagct ggccgaggtc atcggggtcg acaagatcct gttcggttcg gactggccgc
    13261 acggtgaagg cctcgagtcg ccggtgtcgt tcaccgagga actcaagggg ttcagcgacg
    13321 cggacatccg aaaggtcatg cgcgacaacg cgctcgatct cctgggtgtc aaggtcggct
    13381 cggccgcgta gccgcgcaga tgggcgagtg gacgataggg gcggtgctgg acgccatcgc
    13441 cgacgtcata ccggaccgca cgatgactgt ttgtggcaac cggcgaagca catatgccga
    13501 gtcggcagac cggacccgtc ggttagcgaa cttcctcgcg gccaagggat tcggtgccca
    13561 ccgggagcgg gatgcgctcg agcgctggga atgcggtcaa gaccgcatcg cgctgatcat
    13621 ccacaacgac ctctatcccg acatggtgat cggttgcctc aaggcccgaa cggttccggt
    13681 caacgtcaac tacaactacg cctcgcggga gatcggcgag ctgctcgagt acatccaacc
    13741 gcgagcagtc atctatcacc ggtcgttggg cgcgaagttc gccgacgtgc tgccgccgga
    13801 cagcgccgac ctgttgatct cgattgacga cggaagcacg gcgcccgaac tacccggtgc
    13861 cgtcacgttg gatgacgctc tggcgcaagg ggataccgac cagcacagct cgccgtcccc
    13921 ggatgatctg atcatgatct gcaccggcgg taccacgggg cggcccaagg gtgtgctgtg
    13981 gcggcagtcg gacatctatg tgtcaggaat ggtcggagcc gaccatgaag ccgtcgacga
    14041 gatccacggc aaggtgcgcc agacggggcc accgtggttc gcggtgtcgc cgctgatgca
    14101 cgccgccggg atgtggaccg cattctcggc gatcatggcc cgcgcaccgg taatcctgta
    14161 cgacacctgc aagaagctcg acccgcggct ggtgtgggag accgctgagc gggagaaggt
    14221 cgggctgatg acgatggtgg gcgacgcgta cgcggcaccg ctggtcgaag aactccaacg
    14281 cggcacgtac gacctgtcct cgctgtacgc catcggcacc ggcggagccg cgacgaatcc
    14341 taagtatcag cgtgcgctgc tcgaatcgct gccccagctc accctcatca acgggtacgg
    14401 ctcctcggag accggaaaca tgggattcgg ccacagccag caggggaacc agaccgacac
    14461 cttccagct
//
LOCUS       DASWBF010000518        22452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_971627,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000518 DASWBF010000000
VERSION     DASWBF010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22452
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971627"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="murG"
                     /locus_tag="VF874_19195"
     CDS             complement(<1..1008)
                     /gene="murG"
                     /locus_tag="VF874_19195"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX9178101.1"
                     /translation="MSVVLAGGGTAGHVEPAMAVADALRTLDPSVQITALGTARGLET
                     RLVRERGYHLELITPVPLPRKANRDLVRLPLRVRRAVRETRAVLDDVSADVIVGFGGY
                     VALPAYLAAGRRRVPIVIHEANARAGLANRIGARRARRVLAAVPDSGLRGAEVVGVPV
                     RSAITVLDRMAVRAQARAYFGFADDALVLLVFGGSQGAASINRAVAPAAKQLAAAGIS
                     VLHAHGPKNRLDLPEPAEGDPPYVAVPYLDRMDLAYAAADLAICRSGAMTVAEVSAVG
                     LPAVYVPLPIGNGEQRLNALPVVDAGGGLLVDDKDLTPDFVAHTVAALLTDGPRIAAM
                     KA"
     gene            complement(1139..2581)
                     /gene="ftsW"
                     /locus_tag="VF874_19200"
     CDS             complement(1139..2581)
                     /gene="ftsW"
                     /locus_tag="VF874_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX9178102.1"
                     /translation="MLARLLRRGETSGAAKESSTKVGAWLGRPMTSFHLVIAVVALLT
                     TLGLIMVLSASGVRSYDDDGSAWVIFGRQVLWTVIGLVAFYVALRVPVRVMRQTASFS
                     FAFTIFLLVLVLVPGIGKEANGSRGWFVIAGFSMQPSELAKIAFAIWGAHLLAARRME
                     RASLREMLIPLVPAAVVALALIVAQPDLGQSVSLAIIVLGLLWYGGLPLRVFLSSLFA
                     AVSAGAILAVAEGYRSDRVRSWLNPGADPQDSGYQARQAKFALANGGVFGDGLGQGTA
                     KWNYLPNAHNDFIFAVIGEELGFVGAFGLLCLFGLFAYTGMRVARRSADPFLRLLCAT
                     VTMWVLGQAFINVGYVIGLLPVTGLQLPLISAGGTSTATMLFIMGMIANAARHEPEAV
                     AALRAGRDDKVNRLLRLPLPAPYVPTRTEALRDRLRTRPTPEKPPKPKKVAAGAPRGS
                     GHHRVGQRHGGQRRTGKSGGARSRALEGQR"
     gene            complement(2597..4006)
                     /gene="murD"
                     /locus_tag="VF874_19205"
     CDS             complement(2597..4006)
                     /gene="murD"
                     /locus_tag="VF874_19205"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX9178103.1"
                     /translation="MAAGDLLPGRRVLVTGAGITGRAVSAVLAPMGVRLFVCDDDPKA
                     LQRLAEPATVVGTADAAARIADFDLVVTSPGFSPTTPVLAAASAAGVPIWGDVELAWR
                     LDVSGRFGRPRRWLVVTGTNGKTTTTSMLHGMLLAAGRRSALCGNIGSPVLDMLDQPA
                     ELFTVELSSFQLHWAPSLRPEAGAVLNVAEDHLDWHGSFAHYAAAKARALTGRVGVAG
                     VDDAVAARLLADATATLRVGFRLGEPVEGELGVRDGMLLDRAFADAMPLAAASTIPVP
                     GPVGVLDALGAAALARAVDVPAEAIAEALASFRVAPHRAEVVAVVDGVAYVDDSKATN
                     PHAAQASIAAYPRVVWVAGGLLKGASVDEMVRQVANRLVAAVLVGRDRAAVANALSRH
                     APDVPVVEVTTGDDAVVQGASESVGERVMAEVVDAARRLARPGDTVLLAPAGASFDQF
                     TGYAHRGDAFAAAVRGATR"
     gene            complement(4008..5087)
                     /gene="mraY"
                     /locus_tag="VF874_19210"
     CDS             complement(4008..5087)
                     /gene="mraY"
                     /locus_tag="VF874_19210"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX9178104.1"
                     /translation="MRQILIAVGIALTVSILLTPLLVNLFTRQGFGHEIRSDGPPSHK
                     HKRGTPSMGGVAIVAGIWAGYLGTQLVGLALDGNGPSASGLLVLGLATALGAVGFVDD
                     LIKVRKARNLGLNKTTKAVGQIVAAVLFGILVLQFSNADGITPGGPQLSYVREIATVT
                     LVPALFVLFCVVLVSAWSNAVNFTDGLDGLAAGCMAMVTAAYVIITFWQYRNACSTHP
                     GLGCYNVRDPLDLALIAAATAGACVGFLWWNAAPAKIFMGDTGSLALGGIIAGLSVTS
                     RTELLAVVLGALFVAEITSVVVQILAFRTTGRRVFRMAPFHHHFELVGWAETTVIIRF
                     WLLTAIACGLGVALFYGEWLAAIGD"
     gene            complement(5084..6580)
                     /gene="murF"
                     /locus_tag="VF874_19215"
     CDS             complement(5084..6580)
                     /gene="murF"
                     /locus_tag="VF874_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX9178105.1"
                     /translation="MIDLTVAQIAEIVGGELADVSATDAARLHVTGTVEFDSRKITPG
                     GLFLALPGARADGHDHAKAAVQAGAVAVVAARPVGVPAIVVRPSETLDTRASVLEHDV
                     DGSGAAVLAALARLASFVAAELVAGGLTIVGITGSSGKTSTKDLIAAVLAPLGEVVAP
                     PGSFNNELGHPWTVLRATPDTDFLVLEMSARRPGNIAELAAIAPPSVAVVLNVGTAHL
                     GEFGSRDAIAATKAELPQAVPPSGVVILNADDAAVAAMAEMTAARVVRISWETRADVW
                     AEDIHLDELARPQFRLRANDIDVPVQLAVHGEHQVANALCAAAVALECGATGEQVAAA
                     LTQAGPVSAHRMAVTTRADGVTVINDAYNANPDSMRAGLAALSWMANRPDGRRRSWAV
                     LGEMAELGDDAISEHDRVGRLAVRLDVSRLVVVGTGRSMRAMHHGAVIEGSWGGLPTM
                     QATMVDDADAALALLRAELAPGDVVLVKASNAAELGALAESLTRHSTG"
     gene            complement(6577..8055)
                     /locus_tag="VF874_19220"
     CDS             complement(6577..8055)
                     /locus_tag="VF874_19220"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX9178106.1"
                     /translation="MREVAETIGAELVGDVTGDVTGVTLRAQDVQPGDLFAALHGTNA
                     HGAQFAADAVAAGAVAVLTDPAGAAALSVDVPVLVHPAPRSVLGAAASLVSEDPSSKL
                     QVIGVTGTSGKTTTTYLVEAGLRAAGRHAGLIGTVGIRIDGTDQPSALTTPEAPALQA
                     LLASMVEQQVDTVVMEVSSHALALGRVDGVRFAVAGFTNLSRDHLDFHPSMEEYFDAK
                     ARLFDAGSPLRAATAVVCVDDDAGRAMAARAVQPVTVSGEGAEADWRAVDIRTTPDGG
                     QQFVAVDPEGHRHGVAIRLPGRYNVTNSLLALALLRALGVTPEQAAPGLADAQVPGRL
                     ESVDRGQSFIALVDYAHKPGALQAVLENLRRQAGGRLAVVFGAGGDRDPGKRNPMGRI
                     AASWADLVVVTDDNPRSEDPATIRAAILAGTAEATSDAEVVEIGDRRAAIAHAVAWAR
                     PGDVVLIAGKGHETGQTAHGVTRPFDDREELAAALGAITSTA"
     gene            complement(8093..10042)
                     /locus_tag="VF874_19225"
     CDS             complement(8093..10042)
                     /locus_tag="VF874_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9178107.1"
                     /translation="MRHRGSPDRRPADVRRGGRSRDGRAADARRGGTPRSARERRIRP
                     IVQATSQGASFVARHRAGNAVIFLLLIVAAAQLFYLQVPGAPKLRAEAAGQLKVTDVE
                     KAVRGSIVDRNGDKLAFTTQARALTFQPVKVRKELAEAKQKKPDAPDPAQRLVEIANG
                     VAQRLNNRPDAASILKKLQSDETFVYLARAVDPAVATAITTKFPEVGAERQDLRQYPG
                     GSLAANIVGGIDWDGHGLLGLEDAMDGVLAGTDGSITYDRGSDGVVIPGSYRNRHTAV
                     NGSTVELTLDNDIQFYVQQQVELAKGLAGANSVAAVVLDAKTGEVLAMANDNTFDPSQ
                     DIGRQEDRQLGNLPVSSPFEPGSVNKIVTASSVIEFGLSNPDEVLSVPGSINMGGVTV
                     SDAWEHGTMAYTTTGVFGKSSNVGTLMLAQRIGPERYADMLTKFGLGQRTGVGLPGES
                     AGIVPPIDQWSGSTFSNLPIGQGLSMTLLQMTAMYQTIANDGVRVPPRIVRSVVGPDG
                     RRTNEPRPDGIPVVSPQTAQTVRNMLRAVVQRDPMGYQQGTGPQAAVEGYQISGKTGT
                     AQQINPACGCYYSDVYWTTFAGITTTDDPRYVVGIMMDNPHRAADGRPGSSAAPLFHN
                     IASWLLQRENVPLSPDPGPPLTLQA"
     gene            complement(10000..10917)
                     /locus_tag="VF874_19230"
     CDS             complement(10000..10917)
                     /locus_tag="VF874_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178108.1"
                     /translation="MTSRRETLDRPARANRPSRSGPRTTPIRRQARATSTSQAKARAK
                     ARKAKAPKPVREPLRERLIARLASIDLRPQTLVAKVPFVVLVIGSLGLGLALTLWLST
                     DSAERSYQLGNARERNRVLSQQKESLEREVLEAQAAPALAEAARNLGMIPSRDTAHLV
                     QDPAGNWVVVGEPKPAEGAPPPPLNTPLPDPTPPPPPVNRTEVPVMVTRPAPTPAATP
                     AAGAPTPATPEVLVRTPAAAPPPVIPVPTGPLPGPPLNVAGAAAPALNVAGAAAPALD
                     APPPANAPNPQAGPEQSDTVAVPTAVPPT"
     gene            complement(10914..12041)
                     /gene="rsmH"
                     /locus_tag="VF874_19235"
     CDS             complement(10914..12041)
                     /gene="rsmH"
                     /locus_tag="VF874_19235"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX9178109.1"
                     /translation="MTLCATLFDSEAEPHARASWPLPDPTLAHFPSARFASSDRDPGA
                     RALSSSRKRSSVDMSPHVPVLLDRCVELLAPALTRRSPDGLGAVLVDATLGAGGHAER
                     FLAEFGGLKVIGLDRDPDALRTAGERLAGFADRLVMVGARYDEIADVVDGPVDGVLFD
                     LGVSSMQLDRPDRGFSYAADAPLDMRMDPDAQLTAADILNTYDEAALADILHRFGEER
                     FARRIAAQVVRRRASRPFRRTGELVELLYQAIPAPARRTGGHPAKRTFQALRIAVNSE
                     LDSLRAALPAALSALAPGGRIVVMAYQSLEDRIVKSVFAQVTSSRTPQGLPVELPGHE
                     PQFVALTRGAEKAGAAEVERNPRSASVRLRALEKVAGKGDS"
     gene            complement(12016..12447)
                     /gene="mraZ"
                     /locus_tag="VF874_19240"
     CDS             complement(12016..12447)
                     /gene="mraZ"
                     /locus_tag="VF874_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9178110.1"
                     /translation="MFLGTYTPKLDDKGRLTLPAKFRDALAGGLMVTKSQDRSLAVYP
                     RDTFEEMARRASSASRSDPEARAFLRYLAAGTDEQHPDAQGRITLSTDHRRYANLTKD
                     CVVTGSVDYLEIWDARAWQDYQRAHEDDFSAATHDTLRDII"
     gene            complement(12640..13035)
                     /locus_tag="VF874_19245"
     CDS             complement(12640..13035)
                     /locus_tag="VF874_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEX9178111.1"
                     /translation="MPLSDHEQRMLDEIESALYAEDPKFASSVRGGPLRAPSTRRRIQ
                     GTALFVIGLAMLVSGVAFKATMVGGFPILSVLGFLVMFGGVVFAITGPRVSKAAERQV
                     SGGRQRRVKGSGGSFTSRMEDRFRRRFGD"
     gene            13215..13814
                     /locus_tag="VF874_19250"
     CDS             13215..13814
                     /locus_tag="VF874_19250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9178112.1"
                     /translation="MATFLIDLSPRDMQRRLGDALAIYVDAMRYPRGTEDHRASMWME
                     HSRRRGWQAVAAVDVSHGPSGPLPDDEMVSAPMIGVAYGYCGAPDQWWQQQVVAGMRR
                     AGLAPSEIGSLMRSYFELTELHIHPRAQGRGLGEALARRLLSGRGETYVLLSTPEIHG
                     EDNRAWRLYRRLGFTDIIRGHHFAGDPRAFAILGRQLPL"
     gene            13881..14531
                     /locus_tag="VF874_19255"
     CDS             13881..14531
                     /locus_tag="VF874_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3153 domain-containing protein"
                     /protein_id="HEX9178113.1"
                     /translation="MLLVLLLLVPLATGCVRIRASITITPDDRVSGTITAASKPRNGE
                     DKGPQFNGGGLPFSEKVAISDYSREGYVGSEAVFSDLTFAEFPQLASMNHDAAGVDIS
                     LRRAGDSVILEGRADLTSLTDPEADVSLSVSFPGDVTSTNGDQIDSQVVEWQLKPGVV
                     TTMTAQARYTDPSARSFTGAAIWMSLAALAVAAVIGSLAWVSRDRSPRFAEPHEHS"
     gene            complement(14535..15455)
                     /gene="metF"
                     /locus_tag="VF874_19260"
     CDS             complement(14535..15455)
                     /gene="metF"
                     /locus_tag="VF874_19260"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX9178114.1"
                     /translation="MRVTSVVNRIGEVGTQRVPFSVEFFPPRDETAEERLWRAARAFE
                     PLRPAFVSCTYGAGGSTRDRTVRVVRKLASETTLLPVAHMTAVGHSVAELRALVGAFA
                     DCGITNMLALRGDPEGGVHNTWRKHPDGVEYAVELVELIRSLGDFHVGVASFPEGHPR
                     ATDLDHDTANLVAKLRAGAEYSITQMFFGVDDYLRLRDRVHAVDRELGSRPIIPGLMP
                     ITSLRVLRRQVELSGSQIPAPLLQRLTEAAGTGPEENRNEVRKIGIELATEMSTRLLA
                     EGAPCLHFCTLNFARATSEVLTNLGVTASA"
     gene            15494..16585
                     /gene="idsA2"
                     /locus_tag="VF874_19265"
     CDS             15494..16585
                     /gene="idsA2"
                     /locus_tag="VF874_19265"
                     /EC_number="2.5.1.1"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004161 - dimethylallyltranstransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0033384 - geranyl diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (2E,6E)-farnesyl/geranyl
                     diphosphate synthase"
                     /protein_id="HEX9178115.1"
                     /translation="MSVDTAAPSALQLAGAVTDQLREYMSTRRRDAEYIGADYSALTG
                     ALEEFVLSGGKRLRPAFAYWGWRSVTDDEDPENPQMLRLFAALELLHACALVHDDVID
                     ESATRRGLPTVHVRFADVHRRSGWRGSARQFGVSAAILLGDLALVWADDIVATAELSG
                     GARRRVQRVWADIRTEVLGGQYLDIVAEASGVDSIASAMTVNTYKTASYTVSRPLQMG
                     VAAAADRPDVLEAFHGLGTDLGVAFQLRDDVLGVFGDPAVTGKPSGDDLRSGKRTVLL
                     AEAVERAETADPAAAELLRTSIGTELSDDQVQQLCEVITSVGALAAVETRIDALTRRA
                     VDVLSAAPIAEQAKIGLLELARLAANRSA"
     gene            16591..18114
                     /locus_tag="VF874_19270"
     CDS             16591..18114
                     /locus_tag="VF874_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->6)-mannopyranosyltransferase A"
                     /protein_id="HEX9178116.1"
                     /translation="MNRLRAFVSSLDGRPALLGCLGAMMIIAGGLGAGSTRLHDPLLE
                     TLHLSWLRFGHGLVLSSITLWLGVALMLVAWIWLGRWVIDGRATESQMVATTGFWLAP
                     LLLSVPVFSRDTYSYLAQGALLRDGLDPYVVGPVENPNVLLDNVSPIWTTTTAPYGPA
                     FVLVAKFVTMLVGDNVVAGTMVLRLCMLPGLALLIWAAPRIARHIGTSGAAALWICVL
                     NPLVIIHLMGGVHNEMLMVGLMMAGIALTMERHPVPGVALVAAGVAVKATAGLALPFL
                     VWVWVRQLRSGRQLGAVLAFVMAAVGSMVIFVAVFAVLSVAAGVGLGWLTALAGSVKI
                     INWLTVPTAAANLINVVGGLFVGVQFYSVLEMTRLVGIGIIAVALPLLWWRFRHDDRE
                     ALIGIAGVMIVVVLFVPAALPWYYTWPLAVMSALTQSRAWVAAIAGFSTWIMVIFKPD
                     GSHGMYSWIHVGIATACAVLAWRALYKTAEVSTPAPAPAEPLLEPGESAAHQPGERS"
     gene            complement(18032..18436)
                     /locus_tag="VF874_19275"
     CDS             complement(18032..18436)
                     /locus_tag="VF874_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HEX9178117.1"
                     /translation="MSSVPAGDDILDPDEPVYDLPGVAELLGLPVTRVHQQLRDGHLI
                     AVRRGGLVVVPQIFFTDTGHVVKSLPGLLAVLRDGGYRDTEILRWLFRPDESLTVRRD
                     GTREQVTNARPVDALHSHQAREVVRRAQALAY"
     gene            18473..19636
                     /locus_tag="VF874_19280"
     CDS             18473..19636
                     /locus_tag="VF874_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9178118.1"
                     /translation="MIPVEAQEVRPDPLVGALLDGRYRIEAVIATGGMSAVYRGIDTR
                     LDRPVAIKVMDSRYAGDQQFLTRFQLEARAVARLKDPGLVAVYDQGVDGGHPFLAMEL
                     VEGGTLRELLQERGPMPPHAVAAVLRPVLGGLAVAHRSGLVHRDVKPENVLISDTGEV
                     KIADFGLVRALAAAKITSDSVILGTAAYLSPEQVSSGDASPRSDVYACGILVYELLTG
                     VTPFGGDSPLAVAYARMDNDVRPPSIVIQGVPTQFDDLIRHATARNAADRFADAQEMA
                     VELDKIADELGLPEFRVPAPRNSAQHTSAALYLSQPPRQPTREMTHGPQGWGNTERLR
                     APRRFAGIEFSAFQDARQRAKWRVWFWVAAVLILTGLVAAAAWTLGTNIDTLL"
     gene            complement(19633..21021)
                     /locus_tag="VF874_19285"
     CDS             complement(19633..21021)
                     /locus_tag="VF874_19285"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX9178119.1"
                     /translation="MNWTVDIPIDQLPSLPPLPADLRARLDEALAKPAAQQPSWPADQ
                     AKAMRTVLESVPPVTVPSEIQKLAGQLADVAQGKAFLLQGGDCAETFVDNTEPHIRGN
                     IRALLQMAVVLTYGASMPVIKVARIAGQYAKPRSSDIDALDLRSYRGDMINGFAPDAA
                     AREHDPSRLVRAYANASAAMNLVRALTSSGLASLHLVHDWNREFVRTSPAGARYEALA
                     SEIDRGLTFMSACGVDDRNLQTAEIYASHEALVLDYERAMLRLGENEAGEPGLYDLSA
                     HYVWIGERTRQLDGAHVAFAEVIANPIGVKIGPTMTPELAVEYVERLDPHNKPGRLTL
                     VSRLGNNKVRDLLPPIIEKVQATGHQVIWQCDPMHGNTHESSTGYKTRHFDRIVDEVQ
                     GFFEVHRALGTHPGGIHVEITGENVTECLGGAQDISDTDLSGRYETACDPRLNTQQSL
                     ELAFLVAEMLRD"
     gene            complement(21026..21514)
                     /locus_tag="VF874_19290"
     CDS             complement(21026..21514)
                     /locus_tag="VF874_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyadenylate-specific 3'-exoribonuclease AS"
                     /protein_id="HEX9178120.1"
                     /translation="MRYFYDTEFIDDGRLVDLISIGVAAEDGREYYAVSTEFDPQSAG
                     RWVRTHVLPKLPPPSSQIWRSRGQIRSDLEDFFDIDGDEPIELWAWIGAYDHVALCQL
                     WGPMTALPPAIPRFTRELRQHWEDRGSPPLRPRAHDAHDALVDAREALRRFRLIDPGS
                     LP"
     gene            complement(21511..22365)
                     /locus_tag="VF874_19295"
     CDS             complement(21511..22365)
                     /locus_tag="VF874_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178121.1"
                     /translation="MHLTLDFDVVSRGRLPLLWCLIAFLLTFLVTRTVVRYIRAHANS
                     DAPRKWWQPRNIGHGGLHIHHVVIGVVLVMISGITMVTLAVDGGVPEFTAAAIVFGIG
                     AALVLDEFALILHLEDVYWSEDGRTSVDAVFAAVAVAGLLILGFNPLSFFDIGIWRDD
                     HSVLARTTVVLMALATLALAVVVLMKGKVWTGLVGMFISPLLLVGALRLSRPHAPWAR
                     WRYTIRPRKMHRALERERYMRRPVVQAKLWLQDAVSGMPRFPDDARVDEELDREIHAA
                     TPPRRVAA"
BASE COUNT         3410 a         7927 c         7706 g         3409 t
ORIGIN      
        1 ggccttcatc gcggcgattc tcggcccgtc ggtgagcagc gccgccacgg tgtgcgcgac
       61 gaagtccggg gtcaggtcct tgtcgtccac cagcagcccg ccgccggcgt cgaccaccgg
      121 cagcgcgttc aggcgctgtt cgccgttacc gatgggcagc ggcacgtaga cggccggtag
      181 cccgaccgcg gacacctcgg ccaccgtcat cgcacccgac cggcagatcg ccaggtcggc
      241 ggccgcgtag gcgagatcca tgcggtccag atacggcacc gcgacatacg gcggatcgcc
      301 ctcggcgggc tccggcaaat ccaggcggtt cttcggcccg tgcgcgtgca gcaccgaaat
      361 acctgcggcc gcaagctgtt tcgcggcggg agcgacggcc cggttgatcg acgcggcgcc
      421 ctggctgccg ccgaacacca acagcaccag cgcatcgtcg gcgaacccga aatatgcccg
      481 cgcctgcgcg cgcaccgcca ttcggtccag cacggtgatc gccgaacgca ccggcacgcc
      541 gacgacctcc gcgccgcgca gccccgagtc gggcaccgcc gcgagcaccc gccgggcccg
      601 cctcgcaccg atgcgattgg ccaggcccgc gcgggcgttg gcctcgtgga tcacgatcgg
      661 cacgcgccga cgcccggcgg ccaggtaggc cggcaacgcc acgtagccgc cgaagccgac
      721 gatcacgtcg gccgacacgt cgtcgagcac ggcgcgcgtc tcgcgcaccg cccgccgcac
      781 gcgcagcggc agccgcacca gatcgcggtt ggccttgcgc ggcagcggca cgggagtgat
      841 cagctccagg tgatagccac gctcgcgaac cagacgggtc tccagcccgc gcgcggtgcc
      901 cagtgcggtg atttgcacgc taggatcgag cgtccgcagc gcgtcggcga ccgccatcgc
      961 aggctcgacg tgaccggcgg tgccgccgcc ggcgaggacg acggacaacg gcgcgccgtc
     1021 cgtcggagcc gtagggccgg cgacatttgg ccccgcccga tttccgttca cccgctaacg
     1081 ctgacctctg ctcttcgcgc aagcgctcat cgctgccctt tgctcttcac gcaagcgctc
     1141 atcgctgccc ttccaaagcc ctgctgcgcg cacccccgct cttgccggtg cgacgctggc
     1201 cgccatgacg ctggccgact ctatgatgcc ctgaccctcg cggcgcaccg gccgcgacct
     1261 ttttgggctt cggcggtttt tcaggggtcg gccgcgtgcg caggcggtca cgcaacgcct
     1321 cggtgcgcgt cggcacgtag ggcgcgggca gcggcaggcg cagcaggcgg ttcaccttgt
     1381 cgtcgcggcc cgcccgcagc gccgccaccg cctcgggctc gtggcgcgcc gcgttggcga
     1441 tcatgcccat gatgaaaagc attgtggccg ttgatgttcc acccgccgag atcagcggca
     1501 actgaaggcc ggtgacgggc agcagtccga tcacatagcc cacgttgatg aacgcctggc
     1561 ccagcaccca catcgtcacg gtggcgcaca gcagccgcag gaacgggtcg gccgagcggc
     1621 gcgccacccg catgccggtg taggcgaaca acccgaacag acacagcagg ccgaacgcgc
     1681 cgacgaaccc cagctcctcg ccgatgaccg cgaagatgaa gtcgttgtgc gcgttgggca
     1741 gatagttcca tttcgcggtg ccctggccga gcccgtcgcc gaacacaccg ccgttggcca
     1801 gtgcgaactt ggcctgacgg gcctggtagc cggagtcctg cgggtcggca ccggggttca
     1861 accaggaccg cacccggtcg gaccggtagc cctctgccac cgccaggatc gccccggcgc
     1921 tgacggcggc gaacagcgag ctcaagaaca cccgcagcgg cagtccgccg taccacagca
     1981 gccccagcac gatgatcgcc agcgacaccg actgcccgag gtcgggctgc gcgacgatca
     2041 gggcgagcgc caccaccgcc gcgggcacga gtgggatcag catttcgcgc aacgacgccc
     2101 gctccatccg gcgggcggcc agcagatgcg caccccagat cgcgaacgcg atcttggcca
     2161 gctctgaggg ctgcatggag aacccggcga tgacgaacca gccccgagat ccgttggctt
     2221 ccttgccgat tccggggacc agtaccagca cgagcaagaa aatcgtgaaa gcgaaggaga
     2281 acgacgcggt ctgccgcatc acccgcaccg gaacccgcag cgcgacgtag aacgcgacga
     2341 gaccgatgac ggtccagagc acctgccgcc cgaagatcac ccacgccgag ccgtcgtcgt
     2401 cgtaggaccg cacgccggac gccgagagca ccatgatcag cccgagcgtg gtcagcagcg
     2461 ccaccaccgc gatgacgagg tgaaacgacg tcatcgggcg gcccagccag gcaccgacct
     2521 tggtgctgga ctccttggca gccccggaag tctcgccccg gcgcagaagc cgggccagca
     2581 tggcgcgcat cgcgacctac cgggtcgcgc cgcgcaccgc ggcggcgaac gcgtcgccgc
     2641 gatgggcgta gccggtgaac tggtcgaacg acgccccggc gggcgccagc agcacggtgt
     2701 cgcccggccg cgccagccgg cgggccgcgt cgacgacttc cgccatcacc ctttccccaa
     2761 cagactcact ggccccttgc accacagcat cgtcccccgt cgtgacctcc acgacgggga
     2821 catccggggc gtgtcgcgat aacgcgttcg caactgccgc ccggtcccgg cccaccagca
     2881 ccgctgcgac caggcggttt gccacctggc gcaccatttc gtccaccgac gcacccttga
     2941 gcagcccgcc ggccacccac accacccggg gataggccgc gatggatgct tgcgccgcat
     3001 gaggattggt ggccttggag tcgtcgacgt aggcgacccc gtccaccacg gcgaccacct
     3061 ccgcgcggtg cggcgccacc cggaacgacg ccagcgcctc ggcgatcgcc tctgcaggca
     3121 cgtccaccgc ccgggcaagc gcggccgcgc cgagtgcgtc gagcacgccc accggcccgg
     3181 gcaccgggat cgtcgaggcg gccgccagcg gcatcgcgtc cgcgaatgcc cggtccagca
     3241 gcatgccgtc gcgcaccccg agctcgccct caacgggttc accgagccgg aacccgacac
     3301 gcagcgtcgc cgtcgcgtca gccagcaacc gcgcggccac cgcatcgtcg acgccggcga
     3361 cgcccacccg gccggtcagc gccctggcct tcgccgcggc gtagtgcgcg aaggagccgt
     3421 gccagtcgag gtggtcctcg gcgacgttga gcaccgcgcc ggcctccggc cgcaacgacg
     3481 gcgcccagtg cagctggaag ctcgacagct cgaccgtgaa cagctcggcg ggctggtcga
     3541 gcatgtcgag caccgggctg ccgatgttgc cgcacagcgc gctgcgacgg ccggcggcca
     3601 gcagcatgcc gtgcagcatt gacgtggtgg tggtcttgcc gttggtgccg gtcaccacga
     3661 gccagcgccg cggccgcccg aaccggccgg acacgtccag gcgccaggcg agctcgacgt
     3721 cgccccagat ggggacgccg gcggcggacg cagccgcgag caccggcgtc gtcggcgaga
     3781 agccggggct ggtgaccact agatcgaagt cggcgatgcg tgccgcggcg tcggccgtgc
     3841 cgacgaccgt ggccggttcg gctagccgct gcagcgcttt cgggtcgtcg tcacagacga
     3901 acaggcgcac gcccatcggc gcaagtacgg cactcaccgc ccggccggtg atcccggcgc
     3961 cggtcaccag tacccgccgc ccgggaagca ggtcgccggc ggccatgtca gtcaccgatg
     4021 gcggcgagcc actcgccgta gaagagcgcc acgccgagcc cgcaggcgat cgcggtcagc
     4081 agccagaacc gaatgatgac agtggtttcg gcccacccga ccagctcgaa atggtgatgg
     4141 aacggcgcca tccggaacac ccgacggccc gtggtgcgaa atgccaggat ctgcactacc
     4201 accgaggtga tctcggcgac gaacagcgcg ccgagcacca ccgcgagaag ctccgtgcgg
     4261 ctggtcaccg acagcccggc gatgatgccg cccagcgcga gcgagccggt gtcgcccatg
     4321 aagatcttgg cgggcgcggc gttccaccag aggaacccga cgcacgcgcc ggcggtggcg
     4381 gcggcgatca gcgccaggtc cagcggatcg cgcacgttgt agcagcccag acccggatgg
     4441 gtggagcagg cgttgcggta ctgccagaac gtgatgatca cgtatgccgc ggtgaccatc
     4501 gccatgcatc cggcggccag cccgtcgagc ccgtcggtga agttcaccgc gttcgaccac
     4561 gcgctgacca gcaccacaca gaagaggacg aacaacgccg gcaccagcgt gaccgtggcg
     4621 atctcgcgca cgtacgacag ctgaggcccc ccaggcgtta tcccgtcggc gttggaaaac
     4681 tgcagcacca ggatcccgaa cagaaccgcg gcgacgatct ggccgaccgc cttggtcgtc
     4741 ttgttcaacc cgaggttgcg tgccttgcgg accttgatca ggtcgtcgac gaagccgacg
     4801 gcgcccagcg ccgtggccag tccgaggacg agcagccctg acgccgacgg accgttgccg
     4861 tcgagcgcga gaccgacgag ttgcgtgccg aggtagccgg cccagatgcc ggcgacgatc
     4921 gccacgccgc ccatcgacgg agtgccgcgc ttgtgcttgt ggctcggcgg accgtcggac
     4981 cggatctcgt gcccgaagcc ctgccgggtg aacaggttga ccagcagcgg ggtcagcagg
     5041 atcgacaccg tcagggcgat gccgacggcg atgaggattt gcctcatccg gtgctatgtc
     5101 gggtgagcga ttcggccagt gcgcccaatt cggccgcgtt cgacgccttc accaacacga
     5161 cgtcgccggg cgccagctcg gcgcggagca gggcaagggc cgcgtcggcg tcatcgacca
     5221 tggtggcttg catcgtgggt aggccgcccc aggaaccctc gatgaccgct ccgtgatgca
     5281 tggcgcgcat cgacctcccg gttcccacga cgacgagtcg agaaacatct aatcgcaccg
     5341 ccagacgccc gacgcggtcg tgctcggaaa tcgcgtcgtc gccgagctcg gccatctccc
     5401 caagcaccgc ccagctgcgt cgcctgccgt cgggccggtt ggccatccag gacaacgcgg
     5461 ccagccctgc ccgcatcgaa tcggggttgg cgttgtacgc gtcgttgatc accgtcaccc
     5521 cgtcggcgcg cgtcgtcacc gccatccggt gcgccgacac cgggcccgct tgtgtgaggg
     5581 ctgccgcgac ctgctcgcct gtcgcgccgc actccagcgc cacggccgcc gcgcacaatg
     5641 cgttggcgac ctggtgctcc ccgtgcacag ccaactgcac cgggacgtca atgtcgttgg
     5701 cgcgcagccg aaattgcggt cgggccagct cgtcgaggtg gatgtcctcg gcccacacat
     5761 cggcacgtgt ctcccagctg atcctcacca cacgtgccgc ggtcatttcg gccatcgcgg
     5821 cgaccgcagc gtcgtccgcg ttgaggatga ccacgccgga cggcggaact gcttgcggca
     5881 gttcggcttt cgtcgccgca atcgcgtccc gcgagccgaa ctcgccgaga tgggcagtgc
     5941 cgacgttgag caccaccgcg accgacggcg gtgcgatcgc ggccagctcc gcgatgttgc
     6001 cggggcggcg cgccgacatc tccagcacca ggaagtcggt atctggcgtc gcgcgcagca
     6061 ccgtccacgg gtggccgagc tcgttgttga acgacccggg cggggccacc acctcgccca
     6121 ggggcgccag caccgcagcg atcaggtcct tggtcgacgt cttgcctgac gacccggtga
     6181 tgccgacgat cgtcagcccg ccagccacca gctccgctgc cacgaacgac gcgagccgag
     6241 ccagcgcggc cagcaccgca gcgcctgacc cgtcgacgtc gtgctcgagg acgctggcgc
     6301 gggtatcgag ggtttcactc ggtctcacga cgatggcagg caccccgacc ggacgggcgg
     6361 cgaccaccgc gaccgcaccc gcttgcacgg cggccttagc gtggtcatgc ccgtcggcgc
     6421 gggcgccggg cagcgcgaga aacaggccgc cgggcgtgat tttgcgcgaa tcgaactcga
     6481 cggtcccggt gacgtgcagc cgcgcggcgt cggtggccga gacgtcggcc agttcgccac
     6541 cgacgatctc ggcgatctgg gccacggtca agtcgatcac gcggtggagg ttatcgcgcc
     6601 cagcgccgca gccagttcct cgcggtcgtc gaacgggcgg gtgacaccgt gggcggtctg
     6661 cccggtttcg tggcccttgc ccgcgatcag cacgacgtcg ccgggacgcg cccaggcgac
     6721 cgcgtgggcg atggcggcgc gccggtcgcc gatctcgacg acctccgcgt cgctggtggc
     6781 ctccgctgtc ccggccagga tcgccgcgcg gatggtcgcc gggtcctcgc tgcgcgggtt
     6841 gtcgtcggtg acgacgacga ggtcggccca agaagcggca atgcggccca tggggtttcg
     6901 cttgccgggg tcccgatcgc cacccgcgcc gaacaccacc gccagccggc cgccggcctg
     6961 gcgccgcagg ttctccagca ccgcctgcag cgcgccgggc ttgtgcgcgt agtcgaccaa
     7021 cgcgatgaaa ctctgtccgc ggtcgaccga ctcgagccgt cccggcacct gcgcgtcggc
     7081 cagcccgggc gccgcctgct cgggcgtgac gccgagggca cgcagaagag caagcgccag
     7141 aagcgaattc gtgacgttgt accggccggg caggcgaatc gccaccccgt gacggtgacc
     7201 ctcggggtcg acggcgacga actgctggcc gccgtcgggt gtggtgcgga tgtcgaccgc
     7261 acgccaatcc gcttctgccc cttcgccgct gacggtgaca ggctgcaccg cgcgggcggc
     7321 catcgcgcga cccgcgtcgt cgtcgacaca caccaccgcc gtggccgccc gcagcggcga
     7381 gccggcgtcg aaaagccgtg ccttcgcgtc gaagtactcc tccatcgacg ggtggaagtc
     7441 cagatggtcg cgggacaggt tcgtgaagcc ggccaccgcg aaccgcacgc cgtcgacgcg
     7501 cccgagcgcc agcgcgtggc tggaaacctc catcacgacg gtatccacct gctgttcgac
     7561 catcgatgcc agtaacgctt gcagtgccgg ggcctccggc gtggtcagcg cgctcggctg
     7621 gtcggtgccg tcgatgcgga tgccgacggt gccgatcagc ccggcatgcc ggcctgcggc
     7681 acgcagtccc gcctcgacga gataggtcgt ggtggtcttg ccggaggtgc cggtgacgcc
     7741 gatgacctgc aacttcgacg acgggtcctc ggacaccagg gaggccgcgg ccccgagcac
     7801 cgaccgcggc gccgggtgca ccagcaccgg cacgtcgaca gacagggcgg cggcgcccgc
     7861 cgggtcggtc agcaccgcga cggcaccggc cgccaccgcg tcggcggcga actgggcgcc
     7921 gtgcgcgttg gtgccatgca gggccgcgaa caggtcaccc ggctgcacgt cctgggcgcg
     7981 cagcgtcaca ccggtgacgt caccggtgac gtccccgacc agttcggcgc cgatcgtctc
     8041 ggccacttca cgcagcgtga cagacaccct gaaaccgtac ccaggcgcgg tatcaggcct
     8101 gcaacgtcag cggcggcccc ggatcgggtg acagcgggac gttctcgcgc tgcagcagcc
     8161 agctcgcgat gttgtggaac agcggcgcgg ccgacgaccc gggcctgccg tcggcggcgc
     8221 ggtgcgggtt gtccatcatg atgcccacca cgtagcgggg gtcgtcggtg gtggtgatgc
     8281 ccgcgaacgt ggtccagtag acgtcgctgt agtagcaccc gcaggccggg ttgatctgct
     8341 gggcggtgcc ggtcttgccg ctgatctggt agccctcgac cgcggcctgc ggcccggtgc
     8401 cctgctggta tcccatcgga tcgcgttgca ccaccgcacg cagcatgttg cgcacggtct
     8461 gcgccgtctg tggcgacacc accgggatgc cgtccggccg gggctcgttg gtgcgtctgc
     8521 cgtccgggcc gacgacggac cgcacgatgc ggggcggaac ccgcaccccg tcgttggcga
     8581 tggtctgata catcgcggtc atctgcagca gggtcatcga aagcccttgc ccgatcggca
     8641 gattggagaa cgtcgagccc gaccactggt cgatcggcgg aacgatgccg gcgctctcgc
     8701 cgggcagccc gacgccggtg cgctgcccca gcccgaactt ggtgagcatg tccgcgtagc
     8761 gctccggacc gatgcgctgg gcgagcatca gcgtgccgac gttcgacgac ttaccgaaga
     8821 cacccgtggt ggtgtaggcc atggtgccgt gctcccaggc gtcgctgacg gtgacgccgc
     8881 ccatgttgat cgatccgggc accgacagca cttcgtcggg gttggacaac ccgaactcga
     8941 tcaccgagga cgcggtgacg atcttgttga ccgacccggg ctcgaacggc gaggacaccg
     9001 gaaggttgcc cagctgccgg tcctcctgcc ggccgatgtc ctgcgacggg tcgaacgtgt
     9061 tgtcgttggc catcgccagc acttcgccgg tcttcgcgtc cagcaccacc gccgcgacgc
     9121 tgttcgcgcc cgcgagcccc ttcgccagct ccacctgctg ctggacgtag aactggatgt
     9181 cgttgtccag cgtgagctcg accgttgacc cgttgaccgc cgtgtgccgg ttgcggtagc
     9241 tgccggggat caccactccg tcggagccgc ggtcgtaggt gatcgacccg tcggtgccgg
     9301 ccagcacgcc gtccatcgcg tcttccaggc cgagcagccc gtggccgtcc cagtcgatcc
     9361 cgccgacgat gttggcggcc agcgatccgc ccggatactg gcgcagatcc tgacgttcgg
     9421 cgccgacctc cgggaacttc gtcgtaatcg cggtcgccac cgcgggatcc accgcgcgag
     9481 cgaggtagac gaacgtctcg tcgctctgca gcttcttgag gatgctcgcg gcgtctgggc
     9541 ggttgttcag ccgctgcgct accccgttgg cgatctcgac cagccgttgt gccgggtcgg
     9601 gtgcatcggg cttcttctgt ttggcctcgg caagttcctt gcgaaccttg accggctgga
     9661 acgtcagcgc ccgggcttgg gtggtgaacg cgagcttgtc gccgttgcgg tcgacgatgc
     9721 tgccccgcac cgccttctcg acgtcggtca ccttcagctg gcccgctgct tccgcccgca
     9781 gcttgggtgc gccgggcacc tgcaggtaga acagctgcgc cgcggcgacg atcagcagca
     9841 ggaaaatcac cgcgttgccg gcccggtgcc gcgcgacgaa cgacgcgccc tggctggtgg
     9901 cctggacgat cgggcggatt cggcgttccc gggccgagcg cggcgtaccc ccgcgccggg
     9961 cgtcggcggc ccgaccgtcc cgtgatcggc ctccgcgcct cacgtcggcg ggacggcggt
    10021 cgggactgcc acggtgtcgg attgttccgg ccctgcctgc ggattcggcg catttgccgg
    10081 cggcggcgcg tcgagcgccg gagccgcagc gccggcgaca ttcaacgccg gagccgcagc
    10141 gccggcgaca ttcaacgggg gccccggtaa tggaccggtc ggaactggaa tgacgggcgg
    10201 cggcgcggcc gccggtgtgc ggaccagtac ctccggagtg gccggggtcg gcgcgcccgc
    10261 ggccggtgtc gcggcgggtg tgggcgcggg cctggtcacc atcaccggaa cctcggtgcg
    10321 gttgacgggc ggcggcgggg gtgtcggatc cggcaggggc gtgttcagcg gcggtggcgg
    10381 cgcgccctcg gctggcttgg gttcaccgac caccacccag ttgccggccg ggtcctgcac
    10441 cagatgcgcg gtgtcccgcg acgggatcat gccgagattg cgcgcggcct cggccagtgc
    10501 cggtgccgct tgcgcttcga gtacctcgcg ctcgagagat tccttctgct ggctaagcac
    10561 gcggttgcgc tcgcgggcgt tgccgagctg gtaggagcgc tcggcggagt cggttgacag
    10621 ccacagcgtc agagcgaggc ccaacccgag cgacccgatc accaggacca cgaacggaac
    10681 cttggccacc aaggtctggg gccgcaggtc gatcgacgcc agccgggcga tcaaccgctc
    10741 gcgtagcggc tcgcgaacag gcttgggcgc cttggctttc cgtgccttcg cccgcgcctt
    10801 tgcctggctt gtgctggtcg cgcgggcctg ccgccggatc ggtgtggtcc gcgggccgga
    10861 cctggacggg cggttggccc gcgccggacg gtccaacgtc tcgcgcctgc tcgtcatgaa
    10921 tcaccctttc cggcaacctt ttccagcgcc cgcagccgca ccgacgcgct gcgcggattg
    10981 cgctccacct ccgcggcgcc ggccttctcg gcgccgcgtg tcaacgccac gaactgcggc
    11041 tcgtgccccg gcagttcgac cggcagcccc tgcggcgtcc gcgacgaggt cacttgcgcg
    11101 aagacgctct tgacgatccg gtcttccagt gactgatagg ccatcacgac gatgcgtcca
    11161 ccgggggcaa gcgcagaaag cgccgcaggc aacgccgcgc gcaacgagtc gagctcggag
    11221 ttcaccgcga tccgcagcgc ctgaaacgtc cgcttggccg gatgcccgcc ggtgcgccgg
    11281 gccggagccg ggatcgcctg gtagagcagc tcgaccagtt cgccggtgcg gcggaacggg
    11341 cgcgatgcgc gacgacgtac cacctgcgcg gcgatgcggc gggcgaagcg ctcctcgccg
    11401 aagcggtgca ggatgtcggc cagcgcggcc tcgtcgtacg tgttgaggat gtcggcggcc
    11461 gtcagctgcg catccggatc catccgcatg tccagcggcg cgtccgcggc gtagctgaac
    11521 ccgcggtccg gcctgtccag ctgcatcgag gacactccga ggtcgaacag caccccgtcc
    11581 acggggccgt cgacgacgtc ggcgatttcg tcgtaccggg cccccaccat gaccaggcgg
    11641 tcggcgaacc cagccaaccg ctcacctgcg gtccgcagcg cgtcgggatc tcggtcgagc
    11701 ccgatgactt tcaggccgcc gaactcggcg aggaaccgct cggcgtgccc gccggcgccc
    11761 agcgtcgcat ccaccagcac cgcacccaag ccgtcggggc tgcgccgggt gagcgcgggg
    11821 gccagcaact ccacgcagcg gtccagtagg acgggaacgt gcgggctcat gtccaccgat
    11881 gagcgcttgc gcgaagagga aagtgcccga gcacccgggt ccctgtccga tgacgcgaac
    11941 ctggcgctgg ggaagtgcgc cagggtcgga tcgggcagag gccacgatgc acgggcatgc
    12001 ggctcagcct ccgaatcaaa taatgtcgcg caaagtgtca tgggtcgccg cggagaagtc
    12061 gtcttcgtgg gcgcgctggt agtcctgcca ggcccgcgcg tcccagatct ccagatagtc
    12121 aaccgatccc gtcaccacgc agtccttggt caggttcgcg tagcgccggt ggtcggtgga
    12181 cagggtgatc cggccctggg cgtccgggtg ttgctcgtcg gtgccggccg ccaggtagcg
    12241 gaggaatgct cgcgcctcgg gatcgctgcg cgacgcactc gaggcccgcc gcgccatctc
    12301 ctcgaaggtg tcccgtggat agacggcgag gctgcggtct tggctcttgg tgaccatcaa
    12361 ccctcctgcc agcgcgtccc ggaacttcgc gggcagcgtc agccgcccct tgtcgtcgag
    12421 cttgggcgtg taggtgccga ggaacactcc gcgtcctcct gccacgccgt cggaacccct
    12481 ggggccccga catccgccac tttaccccac aatcccccac aacgctcccc atcgtaccaa
    12541 ttcggcgtgt gtcttctcca cctgtcccca cgagcgggtt cagggcacac gaaaaaaggg
    12601 gcgacccacc tgggtcaccc cttcagggga aggaaaatct cagtcgccga agcgacggcg
    12661 gaagcggtct tccatgcggc tggtgaacga accgccggag cccttgacgc gccgctgccg
    12721 tccgcctgac acctgccgct cggccgcctt cgacacccgc ggcccggtga tcgcgaagac
    12781 gacgccaccg aacatcacca ggaacccgag caccgagaga atcggaaacc cgccgaccat
    12841 cgtggccttg aacgcaacgc cggacaccag catggccagc ccgatgacga aaagtgccgt
    12901 gccctggatc cgccggcgcg tgctgggcgc gcgcagcggg ccgccacgca cgcttgacgc
    12961 gaacttagga tcctcggcat acagcgcgct ctcgatctcg tcgagcatgc gctgctcatg
    13021 atcggagagt ggcattcgtc cctccttgcc gccgctgcga tcccgaattg atgcccgcgt
    13081 catcgggcaa ctgacagtca tgatacgagg ccactcggcg ccgtaccaca tagttgatcg
    13141 gccgattcta taggcattca agcgcgcacg ataatggcct tggcgaacga ggacgctcga
    13201 gggaggacct ccaggtggcg acatttctga tcgatctgtc gccgcgcgat atgcaacgcc
    13261 ggctcggcga cgcgttggcg atctatgtcg acgcgatgcg ctacccccga ggcacggagg
    13321 accaccgcgc gtcgatgtgg atggagcaca gcaggcgcag gggctggcag gcggtcgcgg
    13381 cggtggatgt ctcgcacggc cccagcggac cgctcccgga cgacgagatg gtgtccgccc
    13441 cgatgatcgg cgttgcctac ggttattgcg gcgccccgga tcagtggtgg cagcagcagg
    13501 tggtggcggg catgcgccgg gcagggctgg cgccctccga gatcggctcg ctgatgagaa
    13561 gctacttcga gctgaccgag ctgcacatcc accccagagc tcagggccgg gggttgggcg
    13621 aggcgctggc gcggcggctg ttgtccggcc gcggcgagac gtatgtgctg ttgtcgacgc
    13681 ccgagatcca tggggaggac aaccgcgcat ggcggctgta ccggcggttg ggtttcaccg
    13741 acatcatccg cggacaccat ttcgcgggcg acccccgcgc gttcgcgatc ctgggccgcc
    13801 agctccccct ctaggatgac cccgtgctcc ggccccggga atcttcctgc tcctcacgtc
    13861 cgcggcgaca tcgcgcggtt ctgctgctgg tgctgctcct gctcgtgccg ctggccaccg
    13921 gctgcgtccg gatcagggcg tcgatcacga tcacccccga cgacagggtg tccggaacca
    13981 tcaccgcggc gtccaagccg cgcaacggcg aggacaaggg tccccagttc aacggaggcg
    14041 gcctgccgtt ttccgagaag gtcgcgatct ccgactactc cagggaaggc tacgtcgggt
    14101 cggaggcggt gttctccgac ctgacgttcg ccgaattccc ccagctcgcg agcatgaacc
    14161 acgacgcggc cggggtggac atctcgctgc gacgggcggg tgactcggtg atcctggagg
    14221 gccgcgccga tctcacgtcg ttgaccgacc cggaagccga tgtgtcgctg tcggtttcgt
    14281 tccccggcga cgtcacgtcc accaacggcg accagatcga ttcgcaagtg gtcgagtggc
    14341 aactcaagcc gggcgtcgtg acgacgatga ccgcccaggc ccgctacacc gatcccagcg
    14401 cccgctcgtt caccggcgcg gcgatctgga tgagcttggc ggcgctggcc gtcgccgcgg
    14461 tgatcggctc tctggcctgg gtcagccgcg accgctcccc acggttcgcc gaaccgcacg
    14521 agcacagctg acggctaggc cgacgccgtc acgccgaggt tcgtcagcac ctcgctggtg
    14581 gcccgggcga agttcagcgt gcagaagtgc aggcacggcg ccccttcggc cagcagccgg
    14641 gtgctcatct cggtcgccag ctcgatgccg atcttgcgca cctcgttgcg gttctcctcg
    14701 ggtccggtgc ccgccgcctc ggtgagccgc tgcagcagcg gcgccgggat ctgcgacccg
    14761 gacagctcca cctggcggcg cagcacccgc agcgacgtga tcggcatcag cccgggaatg
    14821 atcggccgcg aacccagctc ccggtcgacc gcgtggaccc ggtcgcgcag ccgcaggtag
    14881 tcgtcgacgc cgaagaacat ctgcgtgatc gagtactcgg cgcccgcccg cagcttggcg
    14941 accaggttcg cggtgtcgtg gtcgaggtcc gtcgcccgcg ggtgcccctc cgggaacgac
    15001 gcgacaccga cgtggaagtc gccgagactg cggatcagct cgacaagctc gacggcgtac
    15061 tcgacgccgt ccggatgctt gcgccacgtg ttgtgcacgc ccccctcggg gtcgccgcgc
    15121 agcgccagca tgttggtgat gccgcagtcc gcgaatgcgc cgaccagcgc ccgcagttcg
    15181 gcgacgctgt ggcccacggc ggtcatgtgc gcgaccggaa gcagcgtggt ttcgcttgcc
    15241 agtttccgga cgacgcgcac ggtgcggtca cgggtggaac cgcccgcacc gtaggtgcac
    15301 gacacgaacg ccggccgcag gggctcgaac gcccgcgccg cccgccacag ccgctcctcg
    15361 gcggtctcgt cgcgcggcgg gaagaactcg acggagaacg gcacccgctg cgtgccgact
    15421 tcgccgatcc tgttcactac cgacgtcacc cgcacagcat agaattcgcc atcgcgccgt
    15481 accctgaagg ccgatgagcg tcgacactgc cgcaccgtcg gctctgcaac tggccggcgc
    15541 ggtcaccgac cagcttcgcg agtacatgag cacgcgacgc cgcgacgccg agtacatcgg
    15601 tgccgactac agcgcgctga ccggtgcgct ggaggagttc gtgttgagtg gcgggaagcg
    15661 gctgcggccg gcgttcgcgt actggggctg gcggtccgtc accgacgacg aagaccccga
    15721 aaacccgcag atgttgcggc ttttcgcggc gctggagctg ctgcacgcgt gcgcactcgt
    15781 gcacgacgac gtcatcgacg agtccgccac ccgcaggggc ctgcccaccg tgcacgtgcg
    15841 gttcgccgat gtgcaccgac gcagcggctg gcgcgggtcg gcgcgtcagt tcggggtgtc
    15901 ggcggcgatc ctgctcggcg acctggcgct ggtgtgggcc gacgacatcg tcgccaccgc
    15961 cgagctgtcc ggtggcgccc ggcggcgtgt ccagcgggtg tgggccgaca tccgcaccga
    16021 ggtcctcggc ggccaatacc tcgacatcgt cgccgaggcg agcggcgtgg actcgatcgc
    16081 gtcggccatg accgtgaaca cctacaagac ggcctcctac acggtgtcgc ggccgctgca
    16141 gatgggcgtg gccgcagccg ccgaccggcc cgacgtgctg gaagccttcc acgggctcgg
    16201 caccgatctc ggggtcgcct tccagctgcg tgacgacgtc ctcggagtct tcggagatcc
    16261 cgcggtgacc ggcaagccct cgggcgacga tctgcgctcg ggcaagcgca cggtgctgct
    16321 ggccgaggcc gtcgagcggg ccgagacggc ggacccggcc gccgccgagc tcctgcgcac
    16381 gtcgatcggc accgagctgt ccgacgacca ggtgcagcag ttgtgcgagg tgatcacttc
    16441 cgtgggtgca ctggccgccg tcgaaacccg catcgacgcc ctgacccggc gcgcggtcga
    16501 cgtgctgtcc gccgcgccga tcgctgagca ggcgaagatc ggtctgctcg aactcgccag
    16561 gttggccgcc aaccggtcgg cctgacgagc atgaaccgcc tgcgcgcctt cgtctcgtcc
    16621 ctcgacggcc ggcccgcgct gctggggtgc ctcggcgcga tgatgatcat cgcgggcggc
    16681 ctcggcgcgg gcagcacgcg gctgcacgac ccgctgctgg aaacgctgca cctgtcgtgg
    16741 ctgcggttcg ggcacgggct ggtgctgtcg tcgatcacgt tgtggctcgg cgtcgcactg
    16801 atgctggtcg cgtggatctg gttgggccgc tgggtgattg acggtcgagc caccgagagc
    16861 cagatggttg ccaccaccgg tttctggctc gcgccgctgc tgctgtcggt gccggtgttc
    16921 agccgtgaca cctactcgta cctcgctcag ggtgcgctgc tgcgcgacgg gctggacccg
    16981 tatgtcgtcg ggcccgtcga gaacccgaat gtcctgctgg acaatgtgag cccgatctgg
    17041 acgacgacga ccgcgccgta cggtcctgcg ttcgttctgg tggcgaagtt cgtcacgatg
    17101 ctggtgggcg acaatgtcgt ggccggcacg atggtgctgc gactgtgcat gttgcctggc
    17161 cttgcgctgc tgatctgggc cgcgccgcga attgcccgcc acatcggcac cagcggggct
    17221 gcggcgctgt ggatttgcgt gctgaacccg ctggtaatca ttcacctgat gggcggcgtg
    17281 cacaacgaga tgctgatggt cgggctgatg atggccggca tcgcgctcac catggagcgc
    17341 cacccggtgc ccggtgtcgc gctggtggcc gccggtgtcg cggtgaaggc caccgccggg
    17401 ctggcgctgc cgttcctggt ctgggtgtgg gtgcgccagc tgcgttctgg ccgtcagctc
    17461 ggtgccgtgc tcgcgttcgt aatggccgcc gtcggctcga tggtgatctt cgtcgcggtg
    17521 ttcgctgtgt tgtcggtcgc agccggggtc ggcctgggct ggctcacggc gctggccggt
    17581 tcggtgaaga tcatcaactg gctcacggtg ccgacggcgg cggccaatct gatcaacgtg
    17641 gtcggcggcc tgttcgtcgg cgtgcagttc tactccgtgc tcgagatgac ccggctagtg
    17701 gggatcggga tcatcgcggt cgcgctgcca ttgttgtggt ggcgattccg ccatgacgac
    17761 cgggaagcgc tgatcgggat cgcgggcgtc atgatcgtgg tggtgttgtt cgtgcccgcc
    17821 gcgctgccgt ggtactacac gtggccgctg gcggtgatgt cggcgctgac gcagtcgcgg
    17881 gcgtgggtcg cggcgatcgc tgggttttcg acgtggatca tggtgatctt caaacccgac
    17941 ggctcccacg gcatgtactc gtggatccac gtcggcattg ccaccgcgtg cgcagtgctc
    18001 gcgtggcgcg cgctgtataa aacggccgag gtcagtacgc cagcgcctgc gcccgccgaa
    18061 ccacttctcg agcctggtga gagtgcagcg catcaaccgg gcgagcgttc gtgacctgct
    18121 cgcgggtgcc gtcgcgcctc acggtcagcg actcgtcggg ccgaaacagc caccgcagga
    18181 tctccgtgtc gcggtagccg ccgtcgcgca gcaccgccag cagcccgggc aggcttttca
    18241 ccacgtggcc ggtgtccgtg aagaagatct gcggcaccac gacgagaccg ccccggcgca
    18301 ccgcgatgag gtgcccgtcg cgcaactgct gatgcacccg tgtcaccggg aggccaagca
    18361 actcggcgac tccgggcagg tcgtagaccg gttcgtcggg atcgagtatg tcgtcaccag
    18421 ccggaacgct gctcaccgcg ccgagtctag aacgcgaccc cgccgaacta cgatgattcc
    18481 ggtggaggca caggaggtca gacccgaccc gctggtcggc gccctcctgg acggccgcta
    18541 ccgcatcgag gcggtcatcg ccaccggagg catgtcggcg gtgtaccgcg ggatcgacac
    18601 ccgcctggac cgccccgtcg cgatcaaggt tatggactcg cgctacgccg gcgatcagca
    18661 atttctgacc cggttccagc tcgaggcgcg cgcagtcgcg cggttgaaag accctgggct
    18721 ggtggcggtt tacgaccagg gcgtcgacgg cgggcacccg tttctggcga tggagctcgt
    18781 cgagggcgga acgctgcgag aactgctgca ggaacggggt ccgatgccgc cgcacgcggt
    18841 cgcggcggtg ttgcgcccgg tgctgggcgg cttggcagtc gcgcaccgca gtgggctggt
    18901 gcatcgcgac gtcaagccgg agaacgtgtt gatctccgat accggtgagg tcaagatcgc
    18961 agatttcggc ctggtccgcg cgctcgccgc ggcgaagatc acctcggaca gcgtgattct
    19021 gggcaccgcg gcttatctgt cgccggagca ggtcagcagt ggtgacgcga gcccgcgcag
    19081 cgacgtctac gcgtgcggca tcctggttta cgagctgctg accggcgtca cgccgttcgg
    19141 aggcgacagc ccgttggctg tggcgtatgc gcggatggac aacgatgttc gaccgccgag
    19201 tatcgtaata cagggcgtac caacgcaatt cgacgatctc atccgtcacg cgaccgcccg
    19261 caacgccgcc gaccggttcg ccgacgccca ggagatggcc gtcgagttgg acaagatcgc
    19321 cgacgaactg ggactgcccg agttccgcgt gcccgcgccc cgcaactccg cccagcacac
    19381 ctcagccgcg ctgtacctca gtcagccgcc gcgtcagccg acgcgggaga tgacgcacgg
    19441 tccccagggc tggggtaata ccgaaagact aagagcgcca aggcgattcg cgggtatcga
    19501 gttcagcgca ttccaggatg cccggcagcg cgccaagtgg cgggtgtggt tctgggtggc
    19561 ggcggtgctg attctgacag gcctggtagc ggccgccgca tggacgctgg gcaccaacat
    19621 cgacaccctg ctctagtcgc gaagcatctc cgcgaccagg aacgccagct ccagtgactg
    19681 ctgggtgttt agtcgcggat cgcaggccgt ctcgtagcgc ccggacaggt cggtgtccga
    19741 aatgtcttgc gccccaccga gacactcggt gacgttctcg ccagtgatct ccacgtggat
    19801 gccgcccggg tgggtgccca gcgcgcggtg cacctcgaag aagccctgca cctcgtcgac
    19861 gatccggtcg aagtggcggg tcttgtaccc ggtggacgac tcgtgggtgt tgccgtgcat
    19921 cgggtcgcac tgccagatca cctgatggcc ggtggcctgc accttctcga tgatcggcgg
    19981 cagcagatcc cgcactttgt tgttgcccaa ccggctgacc agcgtcaacc gccccggctt
    20041 gttgtgcggg tccagccgct cgacgtactc gaccgccagc tccggtgtca tcgtcgggcc
    20101 gatcttcacg ccgattgggt tcgcgatcac ctctgcgaac gccacgtgcg cgccgtcgag
    20161 ctggcgggtg cgctccccga tccacacata gtgcgccgac aggtcgtaca gccccggctc
    20221 acccgcctca ttctcgccga gccgcagcat cgcccgctcg tagtcgagca ccagcgcctc
    20281 gtgcgatgcg tagatctcgg cggtctgcag gttgcggtcg tcgacgccgc acgcgctcat
    20341 gaacgtcagc ccgcggtcga tctcactggc cagcgcctcg taacgggcac ccgccggcga
    20401 cgtgcggacg aactcgcggt tccagtcgtg caccaggtgc agcgacgcca ggcccgacga
    20461 cgtgagcgcg cggaccaggt tcatcgccgc gctggcattc gcgtaggcac gcaccaggcg
    20521 cgacggatcg tgttcgcgcg cggccgcatc gggggcgaac ccgttgatca tgtcgccgcg
    20581 gtaggaccga agatcgagtg cgtcgatgtc cgacgatcgc ggcttcgcgt actgacccgc
    20641 gatgcgtgcg accttgatca ccggcatgct cgcgccgtag gtcagcacca ccgccatctg
    20701 cagcagcgcg cggatgttgc cgcggatgtg cggctcggtg ttgtcgacga acgtctcggc
    20761 gcagtcgccg ccctgcagca ggaacgcctt gccctgcgcg acatcggcca gctggccggc
    20821 cagcttctgg atttcggacg ggaccgtgac cggaggcacg ctttccagca ccgtgcgcat
    20881 cgccttcgcc tggtccgccg gccagctggg ctgctgggca gccggtttgg ccagggcctc
    20941 gtccagccgc gcacgcagat ccgcgggcag cggcggcagc gacggcagct ggtcgatcgg
    21001 gatgtcgacg gtccagttca cccgtctatg gtaacgagcc ggggtcgatg agtcggaatc
    21061 gccgcagtgc ctcgcgggcg tcgacgagcg cgtcgtgggc gtcgtgggcc cgcggtcgca
    21121 gcggcgggct gccgcggtcc tcccagtgct ggcggagctc gcgggtgaac cgcgggatgg
    21181 ccggcggcag cgcggtcatc gggccccaca gctggcacag cgcgacgtgg tcgtaggcgc
    21241 cgatccaggc ccacagctcg atgggctcgt ccccgtcgat gtcgaagaag tcctcgaggt
    21301 cgctgcggat ctggccccgg gagcgccaga tctgcgacga cggcggcggc agcttgggca
    21361 gcacatgggt gcgcacccag cggccggctg actgcgggtc gaattccgtt gatacggcgt
    21421 agtattcgcg gccatcctcg gcggcaacgc cgatggagat caggtcgacg aggcgaccgt
    21481 cgtcgatgaa ctcggtgtcg tagaagtacc tcacgcggcc actcgccggg gcggggtggc
    21541 ggcatggatc tcgcggtcga gttcttcgtc gacccgtgcg tcgtcgggga accgcggcat
    21601 gccggacacc gcgtcctgca accacagctt ggcctggacc accgggcggc gcatgtaccg
    21661 ctcccgctcc agcgcccgat gcatcttccg cggtcggatg gtgtagcgcc accgcgccca
    21721 cggcgcgtgc ggacgggaca gccgcagcgc gccgaccagc agcagcgggc tgatgaacat
    21781 cccgacgagc ccggtccaca ccttgccctt catcagcacg accaccgcca acgcaagcgt
    21841 ggccagcgcc atcagcacca cggtcgtcct ggccaggacc gagtggtcgt cgcgccagat
    21901 cccgatgtcg aagaacgaca gcgggttgaa gcccaggatc agcaacccgg ccaccgcgac
    21961 cgccgcgaac accgcgtcga ctgatgtgcg cccgtcctcg gaccagtaca cgtcctccaa
    22021 atgcaagatc aacgcgaatt cgtccagcac cagcgccgca ccgattccga aaacgattgc
    22081 tgcggctgtg aattcgggta ctccgccgtc gacggccagt gtcaccatcg tgatgccgga
    22141 gatcatcacc agaaccaccc cgatcaccac gtggtggatg tgcaagcctc cgtggccgat
    22201 gttgcgcggc tgccaccact tgcgcggagc gtcgctgttg gcatgggcgc ggatgtaccg
    22261 cacgacggtt cgggtcacca ggaacgtgag aagaaatgcg atcaggcacc acagcagggg
    22321 caaccgcccc cgcgacacga cgtcgaagtc cagcgtgagg tgcacgcccg gttacgcggc
    22381 cgccgactgc tcgatgtggt cttcgacctt gccgtccttg acgtcggccg cgtacaggtc
    22441 gacgtactcc tg
//
LOCUS       DASWBF010000519         7179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_788537,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000519 DASWBF010000000
VERSION     DASWBF010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788537"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VF874_19300"
     CDS             <1..528
                     /locus_tag="VF874_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9178122.1"
                     /translation="ARTDADLVVGEPGDGWRVAMGTLTFERGVSTLGQQIVYSRELSG
                     IAELAQRNGAADDPLIRERLARAWVGLRAMRSYALATMDVERRRSRMAGDIYKGQDNV
                     SKLLWANWHRDLGELAMDVLGKAGMALKGGDFDEWQRLFLFSRADTIYGGSNEIQRNI
                     IAERVLGLPREVKGA"
     gene            525..1313
                     /locus_tag="VF874_19305"
     CDS             525..1313
                     /locus_tag="VF874_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9178123.1"
                     /translation="MSLSTVPKEIEGHGLLTGKVVVVTAAAGTGIGSAVARRALAEGA
                     DVVISDHHERRLSETRDELTKLGLGRVESVLCDVTSSVQVDALVASTTARMGRLDVMV
                     NNAGLGGQTPVVDMTDDEWDRVLNVTLTSVMRATRAALQYFRDAPHGGVIVNNASVLG
                     WRAQHSQSHYAAAKAGVMALTRCSAIEAVEYGVRVNAVSPSIARHKFLEKTSSTELLD
                     RLSEGEAFGRAAEPWEVAATIAFLASDYSSYLTGEVISVSSQHP"
     gene            1310..1906
                     /gene="kstR2"
                     /locus_tag="VF874_19310"
     CDS             1310..1906
                     /gene="kstR2"
                     /locus_tag="VF874_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator KstR2"
                     /protein_id="HEX9178124.1"
                     /translation="MTSQPISRRDELLELAATMFAERGLRATTVRDIADRAGILSGSL
                     YHHFASKEEMVDEVLQDFLDWLFARYQHIIDTEPNPLERLKGLFMASFDAIEHRHAQV
                     VIYQDEAKRLLPQPRFAYLEDLNRQQRQMWVDVLQQGIKEGHFRPDLDVDLVYRFIRD
                     TTWVSVRWYQPGGPLTAEQVGRQYLSIVLGGIEAEQEI"
     gene            1918..3066
                     /locus_tag="VF874_19315"
     CDS             1918..3066
                     /locus_tag="VF874_19315"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9178125.1"
                     /translation="MPEAYIIDAVRTAVGKRNGGLAKVHPIDLGVHAFRGLFDRVDVD
                     PGVVDDVIVGCVDAIGGQAGNIGRNAWLAAGYPEEVPGVTVDRQCGSSQQAISFGAQA
                     ILSGTADVILAGGMQNMSQIPIASAMVVGKEFGFTSPTGESENWRHRYGDEEISQFRG
                     TELIAERWNLSREEMEQYALTSHQRALAAIRAGHFDNEIIGVGEFRIDEGPQETSLEK
                     MASLSTLSEGGRLTAAVASQISDGASAVLLASEQAVADHKLKPRARIHHISARGADPV
                     FMLTGPIPATRYALEKTGLSIDDIDVVEINEAFAPVVMAWLKEIKADPEKVNPNGGAI
                     ALGHPLGATGAKLFATMLNELERTGGRYGLQTMCEGGGTANVTIIERL"
     gene            complement(3241..3978)
                     /locus_tag="VF874_19320"
     CDS             complement(3241..3978)
                     /locus_tag="VF874_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178126.1"
                     /translation="MSALAVMLVAMGITDACRNFRLPWVAAAVGPIIVVAVAALSALW
                     HVGDIALLIVAAAATVVWRVLCRCAERTGKHVIAPLVVFFIAAATLIALSGWGSPVSG
                     IVARWMTWAPLPFGAVPPTQLLMAVGVVLLQLVTGNQLVRLVLGSVGAVRPAGQPQPS
                     DRLKGGRLLGPMERLLILGLGLGGQLAAASAVVAAKSIIRFPEINAQKARENGTIGID
                     DITEYFLVGSFASWIVALGGLVLVLAQ"
     gene            complement(3975..4628)
                     /locus_tag="VF874_19325"
     CDS             complement(3975..4628)
                     /locus_tag="VF874_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEX9178127.1"
                     /translation="MTAMNGSASTAVPRAALIGDVVGSRHAADRRGLHRRLTVALKTV
                     AATAIDPPAFTVGDEFQGSYPTVGDAIDAALRMRLAVAPDIDVRFGVGWGEVTVLDEQ
                     ARIQDGPGWWAAREAIEQTAAAQQRPGLALVRTTFRSSGNREDVDAINAALMCRDHLL
                     GSLDDRSLRILRGLMKNQPKKDIAQAEGISPSAVSQRAGRDGLDLIVLASEHLRSVT"
     gene            complement(4680..5693)
                     /locus_tag="VF874_19330"
     CDS             complement(4680..5693)
                     /locus_tag="VF874_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9178128.1"
                     /translation="MATHQAVLVDSTGGPLQLRDLETRSPDRDHIRIDVAACGVCGTD
                     RAIVNGAFPGVSFPLTPGHEIAGAVAEVGQGVDNFSIGDRVAVGWFGGHCNHCIPCRK
                     GLFIHCENMQVPSVQYPGGYAQSVIVPANAAAGIPDALSYAEAAPMGCAGVTTYNALR
                     HTNAVAGDRVAVLGIGGLGHLGVQFARAMGFETVAIARGRDKADDARELGAHHYVDSS
                     AGDVSEALQALGGVTAVLATAGNSQAIGQTVGGLQPRGQLVIVGVAAEPLPISPAQLI
                     MPALSVSGHPSGTARDVEETMDFAVLSGVRARIEERPLAEAADAYAAMEQGRARYRMV
                     LTM"
     gene            complement(5703..6761)
                     /locus_tag="VF874_19335"
     CDS             complement(5703..6761)
                     /locus_tag="VF874_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9178129.1"
                     /translation="MRLHTRLTDLVGSEHPVVQTGMGWVAGARLVSATANAGGLGILA
                     SPTMTLDELASAIRKVKAATDRPFGVNIRADASDADDRVELMIREGVKVASFALAPRQ
                     ELIARLKDAGVVVIPSVGAAKHARKVASWGADAVIVQGGEGGGHTGPVATTLLLPSVL
                     DAVDIPVVAAGGFFDGRGLAAALSYGAAGVAMGTRFLLTSDSTVPDAVKERYLQAGLD
                     GTVVSTRVDGMPHRVLRTSLVDRLESGSRVKGFAAAVRNAAKFKKMSGMTWRTMVRDG
                     LAMRHGKELTWSQVLMAANTPMLLRAGLVEGNTDAGVLASGQVAGILDDLPSCAELIE
                     SIVADAVKHLQAAAALVE"
     gene            complement(6758..>7179)
                     /locus_tag="VF874_19340"
     CDS             complement(6758..>7179)
                     /locus_tag="VF874_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9178130.1"
                     /translation="LQHPTRQMFGVRGAPGNTINHATSYWVGNHSPRVFCDEVDVVSG
                     IGYDKVDPANPAFRYMDVRRVVTNLGVFDFGGPDHTMRAVSLHPGVTAEHVREATSFE
                     VHGLAEADRTRLPKGDELDLIQKVIDPKKLRDKEVRV"
BASE COUNT         1280 a         2408 c         2342 g         1149 t
ORIGIN      
        1 gctcgcaccg acgcggatct ggtggtcggt gagccgggtg acggctggcg cgtcgcgatg
       61 ggcacgctga cgttcgagcg cggcgtgtcg acgctcgggc agcaaatcgt ctattcgcga
      121 gagctttccg gcatcgcgga gttggcgcag cgcaacgggg cggccgacga cccgctgatc
      181 cgcgaaaggc tggcccgggc atgggttggg ctgcgcgcga tgaggtcgta tgcgctggcc
      241 acgatggatg tcgagagacg ccggagccgc atggccggcg acatctacaa aggccaggac
      301 aacgtgtcga agttgttgtg ggccaactgg catcgcgatc tcggcgagct ggcgatggac
      361 gttctcggca aagccggtat ggccttgaaa ggcggagatt tcgacgagtg gcagcggctg
      421 ttcctgttct cgcgcgcgga tacgatctac ggcggttcga acgaaatcca gcgcaacatc
      481 atcgccgagc gggtgctcgg cctgccccgg gaggtcaagg gcgcgtgagc ttgtcaacgg
      541 tccccaaaga gattgaggga catggacttc tgaccggaaa ggtcgtcgtc gtcacagccg
      601 cggccggtac cggaatcgga tcggcggtcg cccgtcgagc gctggccgag ggcgcagatg
      661 tcgtcatctc cgatcaccac gagcggcggc tcagcgaaac ccgggacgaa ctgacgaagc
      721 tcgggctcgg ccgggtggag agcgtgctgt gcgacgtgac gtccagcgtg caggtcgacg
      781 cgctggtggc gtcgacgacc gcgcggatgg gtcgcctcga cgtgatggtg aacaacgccg
      841 ggttgggcgg gcagacgccc gtcgtcgaca tgaccgacga cgagtgggac cgggtgctga
      901 acgtgacgct gacgtccgtc atgcgggcca ctcgcgctgc gctgcaatat tttcgggatg
      961 ctccccacgg cggcgtgatc gtcaacaacg ccagcgtgtt gggttggcga gcgcagcact
     1021 cgcagtcgca ctacgccgcc gcgaaggcgg gcgtgatggc gttgacgcgg tgcagcgcga
     1081 tcgaagccgt cgaatacggg gtgcgggtca acgcggtgtc gccgagcatc gcccggcaca
     1141 agttcctgga gaagacgagc tccaccgagt tgctggaccg gctttccgaa ggcgaggcgt
     1201 tcggccgggc cgccgaaccg tgggaggtcg ccgccacgat cgcgttcctg gccagtgact
     1261 actcgagtta ccttaccggc gaagtgattt cggtatcgag ccagcaccca tgaccagcca
     1321 gccaattagt cgacgcgacg agcttctcga gctcgctgcg accatgttcg ccgagcgggg
     1381 cctgcgcgca accacggtgc gtgacatcgc cgaccgggcc ggcatcttgt cgggcagcct
     1441 ctaccaccac ttcgcgtcga aagaggagat ggtcgatgag gtgctgcaag acttcctcga
     1501 ctggttgttc gcccggtatc agcacatcat cgacaccgag ccgaacccgc tggaacgttt
     1561 gaagggcctg ttcatggcgt cattcgacgc gatcgagcat cggcacgcgc aggtggtgat
     1621 ctaccaggac gaagccaagc ggcttctgcc gcagccgcgg ttcgcgtacc tggaggacct
     1681 gaaccgacag cagcggcaga tgtgggtcga cgtgctgcag cagggcatca aggaaggcca
     1741 tttccggccc gacctggatg tggatctggt gtaccggttc atccgcgaca ccacatgggt
     1801 ttcggtgcgc tggtatcagc ccggcggacc attgacggct gaacaggttg gccgccaata
     1861 tctttcaatc gtcctcggcg gaatcgaagc cgagcaggaa atctagagga gttcgacatg
     1921 cccgaggcgt acatcatcga cgctgtgcgt actgccgttg gcaagcgcaa cggcggcctg
     1981 gccaaggtgc acccgatcga cttgggcgtg cacgcttttc gcggtttgtt cgatcgtgtc
     2041 gacgtggatc cgggcgttgt cgacgatgtg atcgtcggct gtgtggacgc gatcggcggg
     2101 caggcgggca acatcgggcg caacgcgtgg ctggccgcgg gctatccgga ggaggtgccg
     2161 ggcgtcaccg tcgaccgcca atgcggctcg agccagcagg ctatttcctt cggcgcccag
     2221 gcgattctgt ccggtaccgc cgacgtcatc ctcgccggcg gcatgcagaa catgagccag
     2281 atcccgatcg catcggcgat ggtggtcggc aaggagttcg gcttcacgtc gccgacgggg
     2341 gagtcggaga actggcgcca tcgctatgga gacgaggaga tttcgcagtt ccgcggcacg
     2401 gagctgatcg ccgagcggtg gaacctgtcc cgtgaggaga tggagcagta cgcgctgacc
     2461 agccaccagc gggcgttggc ggcgatcaga gcgggccact tcgacaacga gatcatcggg
     2521 gtgggcgagt tccgcatcga cgaggggccg caggagacgt cgctggagaa aatggcctcg
     2581 ctatcaacac tttccgaggg cggcaggctg accgcggcgg tggccagcca gatctccgac
     2641 ggcgccagcg cggtgctgct ggcctcggag caggcggtgg ccgatcacaa gctcaagcca
     2701 cgcgcgcgca tccaccacat cagcgcacgc ggcgccgacc cggtgttcat gctcaccggc
     2761 ccgatcccgg ccacccggta cgcgctggag aagacgggcc tttcgatcga cgacatcgac
     2821 gttgtcgaga tcaacgaggc gttcgcgccg gtggtgatgg cgtggctgaa ggagatcaag
     2881 gccgacccgg agaaggtcaa cccgaacggc ggggccatcg cgttgggaca tccgctcggc
     2941 gcaaccgggg cgaagctctt cgcgacgatg ctcaacgagc tcgagcgcac cggcggtcgc
     3001 tacggcctgc agacgatgtg cgagggcggc ggcaccgcca acgtgacgat catcgaacgg
     3061 ctgtagcccg ttggcgcgag cgtgcgtgtc tgtgccccga cgccgacgaa tgacgtacaa
     3121 gggcgcacgc tcgcgggcgg tgaacgtgca tcgatgacca cacacttagc agcgtgtcga
     3181 tgtacgcacg ctcgcgcggt gacgggccgc acagtcgacg agatggcttg gcgacgaaag
     3241 ctattgcgcg agcacaagta ccagcccgcc gagtgcgacg atccagctgg cgaagctgcc
     3301 cacgaggaag tattcggtga tgtcgtcgat tccgatcgtc ccgttctcgc gggccttctg
     3361 cgcgttgatc tccgggaacc ggatgatgct cttcgccgcc accacggccg acgccgccgc
     3421 cagctgcccg cccagcccca aacccaagat cagcagccgc tccatcggcc cgagcagccg
     3481 gccgcccttg agccggtccg acggctgcgg ctggccggcg ggccgcaccg ccccaaccga
     3541 ccccagcacc agccgcacca gttgattgcc ggtcaccagc tgcaggagca cgacgccgac
     3601 ggccatcagc agctgtgtag gtggcaccgc cccaaacggc aggggggccc acgtcatcca
     3661 tcgcgcgacg atcccgctta ccggagatcc ccagccggac aatgcaatta gtgttgcagc
     3721 ggcgatgaag aacacgacaa gcggtgcgat cacatgcttg ccagtgcgct cggcgcatcg
     3781 gcacagcacc cgccacacca ctgtggccgc cgcggcgacg atcagcagcg cgatatcgcc
     3841 gacgtgccac agtgccgaca gcgccgcgac ggcaaccacg atgatcgggc cgacggccgc
     3901 tgccacccat ggcaggcgaa agttgcggca cgcgtcggtg attcccatcg cgacgagcat
     3961 gacggcgagc gcgctcacgt gacgctccgc aggtgctcgg atgccagcac gatcaggtcg
     4021 aggccatcgc gaccagcgcg ttgcgaaact gcggacgggc tgatcccctc cgcctgcgcg
     4081 atgtctttct tcggttggtt cttcatcagc cctcggagga tccgcaacga tcgatcatcg
     4141 agtgatccaa gcagatggtc ccgacacatc agggccgcgt tgatcgcgtc gacgtcctcg
     4201 cggttgccgc tcgagcggaa cgtcgtgcgc actagcgcca ggcccggtcg ttgctgcgcc
     4261 gccgccgtct gctcgatcgc ctcgcgcgct gcccaccacc ccggtccatc ctgaatccgg
     4321 gcctgctcgt cgagcaccgt cacctcaccc cagcccacgc cgaaccggac atcgatgtca
     4381 ggcgcgaccg ccagccgcat ccgaagcgcc gcgtcgattg catcgcctac ggtcgggtaa
     4441 ctgccctgga attcatcgcc gactgtgaac gccggcggat cgatggccgt cgcggcgacg
     4501 gtcttcagtg ccaccgtcag ccgccggtga agaccgcgac ggtctgcggc gtgacgggaa
     4561 ccgaccacat caccgatcag cgcagcacgc ggaacggcgg ttgaagcgct accgttcatt
     4621 gcagtcatat gaagagcata gcttaagtag ccgaaatttt agctataagc taaagagagc
     4681 tacatcgtca gcaccattcg gtaacgcgcc cggccctgct ccatcgccgc gtacgcgtcc
     4741 gctgcctcgg caagcggccg ttcctcgatc cgggcgcgca caccggacag gacggcgaag
     4801 tccatcgtct cctcgacgtc gcgcgctgtg ccggacgggt ggccgctgac gctgagcgcc
     4861 ggcatgatca gctgcgccgg gctgatcggt aacggttccg cggcgacgcc gacgatcaca
     4921 agctgcccgc gtggctgcaa ccctccgacc gtctgaccga tcgcttgcga gttccccgcg
     4981 gtagccagta ccgccgtgac tccgccgagc gcctgcagcg cttcgcttac atcgccagcg
     5041 ctggaatcga cgtagtggtg ggcacccagc tcgcgggcgt cgtcggcttt gtctctgccg
     5101 cgcgcgatcg ccacggtctc gaagcccatc gcgcgcgcga actgcacccc gagatggccc
     5161 aggccgccga ttcccagcac cgccactcgg tcgccggcaa cagcgttcgt atgccgaagc
     5221 gcgttatagg tggtgacgcc cgcgcacccc atcggcgccg cttcggcgta cgacagcgcg
     5281 tcgggtatcc cggccgcggc gttggctggc acgatcacgg actgtgcata gccgccggga
     5341 tactggacgc ttggcacctg catattctca cagtgaatga aaaggccctt gcggcaaggg
     5401 atgcaatggt tgcagtgacc gccgaaccat ccgacggcca ctcggtcacc gatcgagaag
     5461 ttgtcgacgc cctggcccac ctcagcgacc gcgcccgcga tctcatgccc gggagtcaac
     5521 gggaacgaaa caccaggaaa ggcgccattg acgatcgcgc ggtcggtgcc gcagacaccg
     5581 cacgccgcga cgtcgatgcg gatgtgatcg cggtccggtg accgggtctc caggtccctt
     5641 aactgcagtg ggccgccggt ggagtccacc agaaccgctt gatgtgtggc cattcggcca
     5701 agctactcca cgagcgcggc ggcggcctgc aagtgcttca cggcatcggc gacgatcgac
     5761 tcgatcaact ccgcacacga cggcagatcg tcgaggattc cggccacttg gcccgacgcc
     5821 agcacaccgg catccgtgtt gccctcgacc agaccggctc gcagaagcat gggcgtgttg
     5881 gcggccatca acacctgcga ccaggtcagt tccttgccgt gccgcatcgc cagcccgtcg
     5941 cggaccatcg tccgccacgt cattcccgac atcttcttga acttcgccgc attacgcacc
     6001 gccgcagcga aacccttgac gcgcgaacca ctttccagcc gatcgaccag cgacgtgcgc
     6061 aacacccgat gcggcatgcc gtcgacgcga gtcgacacca ccgtgccgtc caatccggcc
     6121 tgcagatagc gctccttgac ggcatcgggc accgtcgaat ctgacgtcaa caggaaccga
     6181 gtgcccatcg ccacgcccgc cgcaccgtac gacagcgccg cggccaaccc gcgtccgtcg
     6241 aagaaaccgc cggccgccac caccggaatg tcgaccgcgt ccagcaccga cggcaacagc
     6301 agcgtggtcg ccaccggtcc ggtgtggccg ccaccctcac cgccctgcac gatcaccgca
     6361 tcggcgcccc acgacgccac tttgcgggca tgcttggccg cgccgactga cggaatcact
     6421 accaccccag cgtctttcag gcgagcgatc agttcctgcc ggggcgccaa tgcgaacgac
     6481 gccaccttca caccctcgcg gatcatcagc tccacacggt cgtcggcgtc cgacgcatcg
     6541 gcacggatgt tgacaccgaa cggccgatct gtggccgcct tcactttgcg tatcgcagag
     6601 gccagttcgt ccagcgtcat cgtcggcgac gccaggatgc ccagaccgcc cgcgttcgcg
     6661 gtcgcagaca ccaaccgcgc accggccacc catcccatcc cggtctgcac cacgggatgt
     6721 tcactgccga ccaggtcggt caagcgggtg tgcagcctca tactcgtact tccttgtccc
     6781 gcagcttctt cgggtcgatg accttctgaa tcaggtccag ctcgtcgcct ttgggtaacc
     6841 gggtgcggtc cgcctcggcc aacccgtgca cctcgaacga ggtcgcctcc cgcacgtgtt
     6901 ccgcagtgac accggggtgc aatgacaccg cgcgcatcgt gtgatcgggg ccgccgaagt
     6961 cgaagacgcc gagattggtg acgacgcggc ggacatccat gtagcggaag gccggattgg
     7021 ccgggtcgac cttgtcgtag ccgatgccgg agacgacgtc cacctcgtcg cagaacactc
     7081 gcggtgagtg gttgcccacc cagtaactgg tggcgtggtt gatcgtgttg ccgggagcgc
     7141 cacgcacacc gaacatttgc cgcgtcgggt gctgcagcg
//
LOCUS       DASWBF010000520        10724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_451486,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000520 DASWBF010000000
VERSION     DASWBF010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451486"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..1622
                     /locus_tag="VF874_19345"
     CDS             435..1622
                     /locus_tag="VF874_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9178131.1"
                     /translation="MDFSRVELSDDDRAFLDEVRTFLATHITDEVRRCDRETGDNFDE
                     AVHLAAGAAGYLAGELKSESDGGFSPVRRRIWELERCRARMPWFHTGTTVMIARMVAP
                     FASPEIKDEILSGVLSGHVRLCLGFTEPDGGSDVATCKTRAVRDGDGWIINGSKMFTS
                     NAHHSQYVFLLTNTDPDAPKHKSLTMFLVPLDTPGIEIQGIRTVDGDRTNITYYSDVR
                     VDDKYRLGEVNGGWTVLRAALNVEHGVVERDAAGLQKIATMSEHGSLTAEAVDNVAAI
                     AAKKDANGRRLLDDQSVTYRLGRSIARMEAALSTPEMFGRVAIAQTMRDVSPDLMDIL
                     GVASSLPIDADRAADDGAAEYIFRLALPTGVYGGTLEVFRNMIAEHALGLGRPNYSAP
                     AKR"
     gene            complement(1635..2546)
                     /locus_tag="VF874_19350"
     CDS             complement(1635..2546)
                     /locus_tag="VF874_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9178132.1"
                     /translation="MSQDYRFITYERLDDGRIARIMLNRPEARNAQNRGMLVELNDAF
                     LAAEADDDVRVVILGGNGPMFSSGHDLGSSVSRAEYTPGPDQHPSFRMKGATRQGAEM
                     LMLQEWHHFFDNTRRWRNLRKITIAQVHGEVYAAALMLMWACDLIVAAEGTRFADVVG
                     TRLGMCGVEYFAHPWEFGPRKTKELMLTGDSMTVEDAYQLGMVSKIFPGSELGDKTLE
                     FARRIAKVPTMAALLAKEAVNQSVDNMGFYNALNACFTLHQLNHAHWAWVHDNNYPVG
                     LEEDGLPNWRTAPPVTPAQKDKVGPDA"
     gene            2635..4803
                     /locus_tag="VF874_19355"
     CDS             2635..4803
                     /locus_tag="VF874_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9178133.1"
                     /translation="MTTSALAITDEHRDLAESAVGQLSRLDSRAAARATLDGGSSHPA
                     EIWTAAAVLGWNGLAVAEEHGGSGFGLSELAIVLEAQGHELCPGPFLPTVSASLVINR
                     SGLDSLRAQLLPGLADGSTVGALGLSGSVAVGSDLVVSGESPAVLGAPDADVLVIAAG
                     DDVVVVDAAADGVTVTAAEALDTTRSIGTVALREVTVPEDRVLRGVARRARTVFWILA
                     AAEAVGVSWAALEMARDYAKVREQFGRTIGTFQAVKHHLANMLVNAEVATAAAWDAAR
                     GDDLDSAAFAAAVSATQAIRAQIFNAEHNIQLHGGVAFTWEHDAHLYLRRARSLAAVM
                     ADCADPQVDIVDAQRSGMAHGASFTLPPEAEGYRAQAREAASKIRSLPADQQRDALVD
                     SGYLVPHWPKPWGRGADVVEQLVIEEEFRGVDIPDMGITGWVTLTVAQAGTEEQHERW
                     VEPVLRGKEVWCQLFSEPGAGSDAAAVRTAAKRVDGGWQVSGQKVWTSLARQCQWGLA
                     TVRTDPDAPKHAGVTMMAIDMNAPGVQVKPLREITGDSHFNEVYFDDVFIPDSDVVGD
                     VNRGWLVARATLGNERISIGGGSSGTVAFTVDDLIKLLDSAPAATAAAHVRQTGAVIA
                     DFHTLRLLNLRRASRAIAGAEPGPEGNVTKLVLAEQSQRLTELGMELAGTAAVLGQMP
                     KLLQSYLGGRAITIAGGTSEISRNTIAERILGLPRDPLLK"
     gene            4907..6133
                     /locus_tag="VF874_19360"
     CDS             4907..6133
                     /locus_tag="VF874_19360"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9178134.1"
                     /translation="MDEPRWQPPQRPAWVTLLNRVGVTLGSPAALVPLDEQSLLEAAK
                     AATGLEDFGGDEWRRPFRILLADIENEANLTLAGRLLTRYDLVRSLIVRLQMAETEKR
                     HPEILEQPVEAPIFITGMGRTGTSILYELMAQDPRMRATLGWELRFPGHDDRRIADVA
                     AEIDLWLEVVPEFKPIHEMSAEGADEDVVGEMHEFMSQVFTATHRAPNYEAWLVGSGM
                     AQAFRFHRRLLQHLQWKTPGRVIVKGPSHISCLPSLFAEYPDAQVIMTHRDPLKVMAS
                     TANMFATLRWQRSGRVNYAEIVQPLALGYPFLMDMIIQQRSTGVVPDDRFVDVRFADL
                     MLDHLGTISWTYEQLGIDLTDEVADRMRTYLDAKPRARHGSHAYRFDDLGLDQDEMRT
                     KFASYMKHFDIPEEQL"
     gene            6130..7293
                     /locus_tag="VF874_19365"
     CDS             6130..7293
                     /locus_tag="VF874_19365"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX9178135.1"
                     /translation="MTDLDAAWVRYADRIKAAGESITGPDFPTDPRLRAEGYRYVSRL
                     VNLAHQIYLEFGSTTRPSLFRYNDDISTFGAPNTDNNYCRAMVDPTGTYRVTGDISGL
                     KELIVSVHDGEMALGKVAILAEVTLGDLDFGDAGSLELFVGGPEREPNWIPLPEESAY
                     LNIRQFVADWENDPIATLHIERLDDVGPAENVTPEFIAAALDRAATWVETSVAFWNQF
                     SGALKSFTPTNEFSAPRYAEGGAVNMVHGGTRWELDENQALVVEFDQPQATYWSIQTY
                     MFHWLAPLDFRNRVTSLNDTQVHVDDDGKVRVVISHQDPGVQNWLDASGLREGLCSYR
                     WVRATTQPTPVATLVAAADVRKHLPPSAPEFSAAERKQQIAARQRGFDRRFRR"
     gene            complement(7290..7979)
                     /locus_tag="VF874_19370"
     CDS             complement(7290..7979)
                     /locus_tag="VF874_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX9178136.1"
                     /translation="MSLVAGRGPLSKDPAGWFAPPLLSDVVYVEPHPRRVQGIRNGQR
                     VIDTEHALLVHRRDRPLSYAFPANEVGDLPNEPEPDAPGYVQVPWDAVDTWLEEGRKL
                     VHYPPNPYHRVDCRPTRRALRVAVDGTTLVDTDDTVIVFETALEPRLYVDPSLVRVDL
                     LRRTETTSYCNYKGVATYWAAVIGDTVVDDVAWSYEDPPPETLPIKGYLSFDAERADV
                     LAELPRRDPAL"
     gene            complement(7976..8590)
                     /locus_tag="VF874_19375"
     CDS             complement(7976..8590)
                     /locus_tag="VF874_19375"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9178137.1"
                     /translation="MPESLRERSRARRRAEIERAALRLFAERGYDATTVAQVAEAAEV
                     SPRTVSLYFPSKLELALSYIADSAQRLSEACANRSADQTTLDILAGWLQDERKTHGQT
                     IGLLRAMLDANPALRGAETPEIAESKRKVNEALAADLGRSSDDAVVRLVNGAMAGVIS
                     ALLQIDPKADGAAEAFDVANRLFKSVIRSAEARKGKATRVRTAG"
     gene            8692..9813
                     /locus_tag="VF874_19380"
     CDS             8692..9813
                     /locus_tag="VF874_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9178138.1"
                     /translation="MSMARLAALVGRGVQRLATDAVLQGARSFPRRVGDLDAEALSRI
                     MGRRVSSVSVLDGAAGTSSRARLALTGDDIPGSVFVKMSAATAATRMLGELARLGETE
                     ARFYKQLAPELDSGVPRCYGSEWDPLTGRYVIVLEDMTLTQCEFPDTLNPLDNDKMAL
                     LVEVLAHLHGTFWGRLPEKPSGEGQFGWLWPPSADPSVPLTPHLMRLSARRLADRTPI
                     PVDAGRFIWENFQAATAVVDEGPHTVLHGDSHPGNTFFRDGRAGLLDWQVVRRGHPAR
                     DLAYTLVLGMTTDDRRAIQRDLLTVYRRALAASGGPELDHDELWTRYRQAVAYAYVSP
                     LTTAGLGGMQTEEISLEGLRRAVAALDDLETVQAVREAL"
     gene            complement(9817..10482)
                     /locus_tag="VF874_19385"
     CDS             complement(9817..10482)
                     /locus_tag="VF874_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9178139.1"
                     /translation="MTRSAVVRDGDEPLARRAESSDEPLARRAESSDEPLARRAESSD
                     HVDDDTSTRQRLLVATSEVLSRKGMTKLSLSEVAAQAGVSRPTLYRWFASKEELLDAF
                     GVWEREMYDRGIAKATAGLRGREKLDAALRFIVDYQHSYSGYRMVDIEPDHVIGQLSH
                     VIPVMRERLLRLLPGPNSAVAAATAIRVAVSHYLVASDDADQFLAQLRHAVGLKHPPA
                     DDT"
     gene            10571..>10724
                     /locus_tag="VF874_19390"
     CDS             10571..>10724
                     /locus_tag="VF874_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9178140.1"
                     /translation="MAGPLEGINVVELGVWVAGPAAGGILADWGAEVVKIEPPRGDPA
                     RMFGRML"
BASE COUNT         1853 a         3443 c         3583 g         1845 t
ORIGIN      
        1 ccggaaccgc ctcgggcgtg cagacgggga gtccgatacc gggcccggtc acgttcaaga
       61 tcgtcgcgag atgctgaccg gccggaacga gcggttgaaa ccgcgagagc ggggtcgtcg
      121 gttcaccatc gaggacagtc aggagtggac gagatgacgg cgatcgacag ctgccccggg
      181 tgcggctatc cgaagttcgg tcctggtctg tgtgcgtact gccaaccggt gctggcgctg
      241 gatcaagaca gtcgcattcc ggcacgagaa tcagctggcg gaggagcagc agcggggttc
      301 ggtgacgata tggccgccgt gcccgcggtg tgacgggccg cagccgcctt tacaaaaatt
      361 ttgaaatact gtcacgctcg actcatgccg ggccactatg aagcgtgcag gggtgatcga
      421 ggacggagcc gccggtggat ttctcccgcg tagaactgtc cgatgacgac cgggctttcc
      481 ttgacgaggt tcggaccttt ctcgccaccc acataaccga cgaagtgcgc cggtgcgacc
      541 gcgagaccgg cgacaacttc gacgaagccg tgcatctggc cgcgggcgcc gcaggttacc
      601 tcgcgggcga gttgaagtcc gaatccgacg gcgggttcag cccggtgcgc cggcgcatct
      661 gggagctgga gagatgccgg gcgcgcatgc cgtggtttca cacgggtacg acggtcatga
      721 tcgcgcggat ggtggcgccg tttgcatcgc cggagatcaa ggacgagatc ctctcaggtg
      781 tgctgtccgg ccacgtccga ctgtgcctgg gcttcacgga gcccgacggt ggctcggatg
      841 tcgcaacatg caagacccgg gcggtgcgtg acggtgacgg ctggatcatc aacgggtcga
      901 agatgttcac gtccaacgca caccacagcc agtacgtctt tctgctcacc aataccgatc
      961 cggatgcgcc aaaacacaag agcctgacca tgtttctggt cccgctcgat acgcccggca
     1021 tcgagatcca gggcatccga accgtggacg gcgaccgaac gaacatcacc tactacagcg
     1081 atgtgcgcgt cgacgacaag tatcggctgg gtgaggtgaa tggtggctgg acggtgctgc
     1141 gtgcggcgct gaacgtggaa cacggcgtcg tcgagcgcga cgccgcgggc ctgcagaaga
     1201 tcgcgaccat gtccgaacac ggctcactga cggccgaggc ggtggacaac gtcgccgcga
     1261 ttgcagccaa aaaagatgcg aacggccggc gactgctcga cgaccagtcc gtcacatacc
     1321 ggctcggccg cagcattgcc cggatggagg ctgcgctcag cacaccggag atgttcgggc
     1381 gtgttgcgat cgcgcaaacg atgcgcgacg tcagccccga cctgatggac atcctcggcg
     1441 tcgcgtcgag cctgcccatc gatgccgaca gggccgccga tgacggagcg gcggagtaca
     1501 tcttccggct ggcattgccc accggcgtct atggcggcac cctggaggtg ttccgcaaca
     1561 tgatcgccga acacgccctc ggcctgggcc gccccaacta ctccgcgccg gcgaagcggt
     1621 aatcggttgg ggcgctaggc gtccggcccg accttgtcct tttgcgccgg agtaaccggc
     1681 ggcgcggtcc gccagttcgg caggccgtcc tcttcgaggc cgacggggta gttgttgtcg
     1741 tgtacccaag cccagtgagc gtggttgagc tggtggagcg tgaaacacgc gttgagcgcg
     1801 ttgtagaagc ccatgttgtc gacgctttgg ttcaccgcct ccttggcgag cagcgcagcc
     1861 atcgtgggga ccttcgcgat tcggcgagcg aactccagtg tcttgtcgcc taactcgctc
     1921 cccggaaaga tcttcgacac catgccgagc tggtatgcgt cttcgacggt catggaatca
     1981 ccggtgagca taagctcttt ggtcttccgg ggcccgaatt cccaagggtg ggcgaaatac
     2041 tcgacgccgc acatgcccag ccgggtaccg acgacatctg cgaagcgggt gccttcggcg
     2101 gcgacgatga gatcgcaggc ccacatcagc atcagcgccg cggcgtagac ctcaccgtgg
     2161 acctgggcga tggtgatctt gcgcaggttg cgccagcggc gggtgttgtc gaagaaatgg
     2221 tgccactcct gcagcatcag catttccgca ccctggcggg tcgcaccttt catgcggaag
     2281 ctggggtgct ggtcgggccc gggcgtgtac tcggcgcggg acacactcga ccccaggtcg
     2341 tgaccggagg agaacatcgg cccattcccg cccagaatga ccacccggac gtcgtcatcg
     2401 gcttccgccg ccagaaatgc gtcgttcagt tcgacgagca tcccgcggtt ctgcgcattc
     2461 cgcgcttcgg ggcggttgag catgatccgg gcgatacggc cgtcgtcgag ccgttcatag
     2521 gtgatgaagc gatagtcctg tgacatagcc gcatcctatg cacgcgagaa ccacatcctc
     2581 caaaaaggag catcctgttc accgagcgcg ggacctcggc gttaccgtct tcctgtgact
     2641 acctcagcgt tagcgatcac cgacgaacac cgcgacctcg ccgaatcagc ggttgggcag
     2701 ctgagcaggc tcgacagccg cgcggcggct cgcgcgacgt tagacggtgg ctcatcccat
     2761 ccggccgaga tctggaccgc ggcggccgtg ctgggctgga acggtctggc ggtcgccgag
     2821 gagcacggcg gctcgggatt cgggctgtcc gagctggcga tcgtgctcga ggcgcaaggc
     2881 cacgagctgt gccccgggcc gttcctgccg accgtctcgg catcgttggt catcaaccgc
     2941 agcggtttgg attcgcttcg cgctcaattg cttccgggtc tcgccgacgg cagcacggtg
     3001 ggtgcgttgg ggttgtccgg cagcgtggcc gtgggctcgg acctggtggt gagcggcgag
     3061 agtcccgcgg tgctgggcgc gcccgacgcc gacgtgctgg tgatcgccgc cggcgacgac
     3121 gtcgtcgtgg tcgatgccgc cgcggacggg gtgaccgtca ccgcggcgga ggcgctggac
     3181 accacccgca gcatcggcac cgtcgcgcta cgcgaggtca cggtgcccga agaccgggtg
     3241 ctgcgcggtg tggcccgccg ggcgcgcacg gtgttctgga tccttgccgc cgcggaggcc
     3301 gtcggagtca gctgggccgc cctggagatg gcgcgggact acgcaaaagt gcgtgagcag
     3361 ttcggccgga ccatcggcac gttccaggcg gtcaaacacc atctcgcgaa catgttggtc
     3421 aacgcggagg tggccacggc cgccgcgtgg gacgccgccc gcggcgacga cttggacagc
     3481 gccgcattcg ctgccgcagt gtccgccaca caagcgattc gtgcgcagat cttcaacgca
     3541 gagcacaaca ttcagcttca cggtggcgtc gcattcacct gggagcacga tgcgcaccta
     3601 tacctacggc gtgcccgttc tctagcggcg gtgatggccg attgcgccga cccacaggtc
     3661 gatatcgtcg acgcgcagcg cagcgggatg gcacacggcg cgtcgttcac gctgccgccg
     3721 gaggcggaag gctatcgggc gcaggcacgc gaagccgcca gcaagatccg ttcactaccg
     3781 gcggaccagc agcgcgatgc gctcgtcgac tccggttatc tcgtaccgca ctggccgaag
     3841 ccgtggggca ggggagccga cgtcgtcgag cagttggtga tcgaggaaga gttccgcggt
     3901 gtcgacatcc cggacatggg aatcactggg tgggtgacgc tgaccgtcgc gcaggcaggc
     3961 accgaggagc agcacgagcg ctgggtggaa cctgtgctgc gcggcaagga agtgtggtgc
     4021 cagctgttct cggaaccggg cgcgggttct gacgctgcgg ccgtacgcac cgccgcaaag
     4081 cgcgtggacg gcgggtggca ggtcagcggg cagaaggtgt ggaccagcct tgccaggcaa
     4141 tgccaatggg gtcttgcgac ggtgcgcacc gacccggacg cgcccaagca tgccggtgtc
     4201 acgatgatgg ccatcgacat gaacgcccct ggcgtgcaag tgaaaccgct gcgggagatc
     4261 accggcgact cgcacttcaa cgaggtgtac ttcgacgacg tgttcattcc ggactccgac
     4321 gtggtgggcg acgtcaaccg cggctggttg gtggcccgtg cgacgttggg caacgagcgc
     4381 atctcgatcg gcggtggctc gtcgggcacg gtcgcgttca cggtcgacga cctgatcaag
     4441 ctgctggaca gcgcgcccgc cgcgaccgcg gcggcccatg tgcggcagac cggtgcggtg
     4501 atcgccgatt tccacacgct gcggctgctc aatctgcgcc gggcgagccg agcgatcgcc
     4561 ggcgccgaac cggggcccga aggcaacgtg accaagctgg tgctggctga acagtcccaa
     4621 cgcctgaccg agctgggcat ggagttggcc ggcacggctg ccgtgctcgg gcagatgccg
     4681 aagctgctgc agtcgtacct gggcggtcgc gcgatcacga tcgccggcgg cacgtcagag
     4741 atctcgcgca acacgatcgc cgaacgcatc ctcggtctgc cgcgagaccc tctgctgaaa
     4801 tagcgggcca tcacatcgtc acgtgagcgg gagaaggcga tgcgaacata attactgctg
     4861 tctgtaaact tgctgctgag tgcattcctg cgataagttg taacgcatgg atgaaccccg
     4921 gtggcagccg ccacaacgcc cggcgtgggt gaccttgctg aaccgagtgg gcgtcactct
     4981 cggttcaccg gcagcgctgg tgcccctcga tgagcaatcg cttctcgaag cggcgaaggc
     5041 ggcgaccggt ctcgaggact tcggtggcga tgaatggcgg aggccgttcc gaatcttatt
     5101 ggccgacatc gaaaatgaag caaacctgac tcttgccggc cggctgctga cgcgctacga
     5161 cttagtccgt tcgctcatcg tccgcttaca gatggccgag accgagaagc gccaccccga
     5221 gatcctcgaa caacccgtcg aggcaccgat cttcatcacc ggcatgggcc gcacgggtac
     5281 gagcatcttg tatgagctga tggcgcagga tccgcgcatg cgggcgactt tgggttggga
     5341 actgcgcttt cccgggcacg atgaccgccg catcgccgac gtggccgcgg aaatcgacct
     5401 gtggctcgag gtcgtcccgg agttcaagcc gatccacgag atgtccgccg aaggagccga
     5461 cgaggacgtc gtcggtgaaa tgcacgagtt catgtcgcag gtgttcaccg cgacgcaccg
     5521 cgccccgaac tacgaggcgt ggttggtggg cagcggcatg gcgcaggcgt tccggttcca
     5581 ccggcgcctt ctgcagcacc tgcaatggaa gacacccggg cgcgtcatcg tgaagggccc
     5641 gtcgcacatc tcctgcttgc caagcctttt cgccgagtac ccggacgccc aggtgatcat
     5701 gacgcaccgt gatccgctca aggtcatggc gtcgaccgca aacatgttcg ccacactgcg
     5761 ctggcagcgc tcgggccgag tgaactacgc cgagatcgtc caaccgctcg cgctgggtta
     5821 cccgttcttg atggacatga tcatccagca gcggtcgacc ggcgtcgttc cggacgaccg
     5881 cttcgtggac gtgcgcttcg ccgacctaat gctggatcac ctcggcacca tcagctggac
     5941 ctacgagcag ctcggcatcg acctgaccga cgaggtggcc gatcgcatgc ggacgtacct
     6001 cgacgccaaa cccagggctc gacacggctc ccatgcgtac cgcttcgacg acctcggact
     6061 tgaccaagac gagatgcgga cgaagttcgc gtcgtacatg aagcacttcg acattccgga
     6121 ggagcaactg tgacggacct tgacgcggca tgggtgagat acgccgaccg gatcaaggct
     6181 gccggcgaaa gcatcaccgg gcctgacttc ccaaccgatc cacggcttcg cgccgagggc
     6241 taccgctacg tgagccggct ggtcaacctg gcgcaccaga tctacctcga attcggcagc
     6301 accacaaggc cgtcgctgtt ccgctacaac gacgacatca gcacgttcgg cgcccccaac
     6361 acggacaaca actactgccg tgccatggtc gacccgactg ggacctaccg tgtcaccggc
     6421 gacatctcag gcttgaagga gctgatcgtc tccgtccatg acggcgagat ggcgctcggc
     6481 aaggtcgcca tcctcgccga ggtgacgctc ggagacctgg acttcggcga cgccggctcg
     6541 ctcgagctgt tcgtcggcgg ccctgagcgc gagccgaatt ggattccgct gcctgaagaa
     6601 tcggcgtacc tcaacattcg ccaattcgtg gccgactggg aaaacgaccc aatcgcgact
     6661 ttgcacatcg agcgactgga cgacgtgggg cccgcggaga atgtgacgcc ggagttcatc
     6721 gcggccgctc tcgaccgggc ggcgacatgg gtggaaacca gcgtcgcgtt ctggaaccag
     6781 ttctccggcg cgctcaagtc gttcacgccg acgaacgagt tcagcgcgcc gcgctatgcc
     6841 gagggcggcg cggtcaacat ggtgcacggt ggaacgcgct gggagctcga cgagaatcag
     6901 gcgctggtgg tggaattcga ccaaccgcag gccacctatt ggtcgatcca gacgtacatg
     6961 ttccactggc tggcaccgct ggacttccgt aaccgggtca cgagcctcaa cgacacccag
     7021 gtgcacgtcg acgacgacgg caaggtccgc gtcgtgatct ctcaccagga tcccggtgtg
     7081 cagaactggc tcgacgcgtc gggcctgcgg gaggggctgt gctcgtaccg atgggtgcgg
     7141 gcgaccacgc aacccacccc cgtcgcgacg ctcgttgccg ccgccgacgt ccgcaagcac
     7201 ctgccgccgt cggcacccga attttcggcc gccgagcgca agcagcagat cgctgctcgg
     7261 cagcggggtt tcgatcggcg tttccggcgc tagagtgcgg gatctcgacg aggcaattcg
     7321 gcgaggacgt cggcgcgctc ggcgtcaaag ctgagatagc ccttgatggg aagggtttcc
     7381 ggtgggggat cctcgtagct ccaggcgaca tcgtcgacga cggtgtcgcc gatgacagcc
     7441 gcccaataag tcgctacacc tttgtagttg cagtagcttg tcgtctccgt ccggcgcaag
     7501 agatccacgc ggaccaacga cggatcgacg tagagccggg gttccagcgc ggtttcgaag
     7561 acgatcaccg tgtcgtcggt gtccaccagc gtggtgccgt cgaccgcgac gcgcagggcg
     7621 cgtcgtgtcg ggcggcagtc gacacggtgg tacgggttcg gcgggtagtg gacgagcttg
     7681 cgaccttctt cgagccaggt gtcgactgca tcccacggca cctggacgta gccgggcgcg
     7741 tcgggttcag gctcgttcgg cagatcgcca acctcgttgg ccgggaacgc atagctgagc
     7801 ggacggtccc ggcggtgcac caacaacgca tgctcggtgt caataaccct ctgcccgttg
     7861 cgaattcctt ggacgcgacg cggatgcggc tcgacataga ccacgtcaga gagcagcggg
     7921 ggagcgaacc atcctgcggg gtccttgctg agcgggcccc gtccggcgac gaggctcacc
     7981 ctgcagtcct gacccgcgtc gctttgccct ttcgggcttc cgccgaccgg atcaccgact
     8041 tgaagagccg attcgcgacg tcgaaagcct cggcggcacc gtctgctttc gggtcgattt
     8101 gcagcagggc ggagatgaca ccggccatcg ccccattgac aagccgcacc acagcgtcgt
     8161 cggacgaccg tcccaggtcg gcggcaagcg cttcgttgac cttgcgcttg gattcggcga
     8221 tctcgggcgt ttccgcgccc cgcagtgcgg gattcgcatc gagcatcgcg cgtagcagcc
     8281 cgatcgtctg gccatgtgtt ttccgttcgt cctgcagcca gccggccagg atgtcgaggg
     8341 tggtttggtc ggcgctgcgg ttcgcgcatg cctcgctgag ccgttgcgct gagtcggcga
     8401 tgtaggacag cgcgagctcg agcttggacg gaaagtacag cgacacggtg cgcggagaca
     8461 cctcagcggc ctccgcgacc tgtgcgaccg tcgtcgcgtc gtagccgcgt tcggcgaaga
     8521 gccgcagtgc agcgcgctcg atttcagctc ggcgtcgcgc ccgcgaccgc tcacgcaatg
     8581 actcaggcat gagcgttact ttacagcgac atgcaaagtt accgctagtt gcaataatgc
     8641 ctttacaacg gctgccaaaa gtgtcaccct gccgatgtga ggaaacaggc gatgtcgatg
     8701 gcgcgattgg cggcgcttgt cggacgcggc gttcagcgac tggccaccga cgcggttttg
     8761 caaggcgcac gaagcttccc gcgccgtgtc ggcgacctcg acgccgaggc gttgtcgcgg
     8821 atcatgggcc gccgcgtctc ctcggtgtcg gtgctcgacg gcgctgcggg aacatcttct
     8881 cgggcccgcc tggcgctcac cggcgacgac attccaggtt cggtcttcgt gaagatgtcg
     8941 gcggccaccg cggccacgcg gatgctcggc gaactggctc ggctcggcga aaccgaggcc
     9001 cggttctaca agcagctggc acccgagctc gactccgggg tcccgcgctg ctacggatcg
     9061 gagtgggatc cgctgaccgg ccggtacgtc atcgtcctcg aggacatgac gctgacgcag
     9121 tgcgagttcc ccgacacgtt gaatccgctc gacaacgaca agatggcgct tctcgtcgaa
     9181 gtccttgcgc acctgcacgg cacgttctgg ggccgactgc ccgaaaagcc cagtggcgaa
     9241 gggcaattcg gctggctgtg gcccccctct gccgatccga gcgttccgct gactccgcac
     9301 ctgatgcggc tgtcggcgcg ccgtctggcc gatcggacgc cgattcccgt cgacgcgggc
     9361 cgcttcatct gggagaactt ccaagcagcg accgcggtgg tcgacgaagg tccgcacacc
     9421 gtgctgcacg gcgactccca tccggggaac accttcttcc gcgacggtcg agcgggcctg
     9481 ctggactggc aggtcgtccg ccgcggccac cctgcccgcg acctcgcgta cacgttggtc
     9541 ctcggcatga ccaccgacga ccgtcgggct attcagcgcg acctattgac cgtctaccga
     9601 cgggcgttgg cggcgagcgg cggtcccgag ctcgaccacg acgagctgtg gacccgctac
     9661 cggcaagctg tcgcctacgc ctacgtctca ccactcacga cggccggcct cggcggcatg
     9721 caaaccgaag agatctccct cgaaggatta cgacgggcag tcgccgcact ggacgacctc
     9781 gaaaccgtgc aagccgttcg ggaggcgctg tgaccgtcag gtgtcgtccg cgggcgggtg
     9841 cttgagcccg accgcgtggc gaagctgggc gaggaattgg tcggcatcgt cgctggcgac
     9901 caggtaatgc gacaccgcga cgcggatcgc cgtcgcggcc gcgaccgcgc tgttggggcc
     9961 gggcaggagc cgtagcagtc gctcgcgcat gaccggaatc acgtgggaca actgcccgat
    10021 gacgtgatcg ggttcgatgt cgaccattcg gtatcccgag tacgaatgct ggtagtcgac
    10081 aatgaatctc agcgcagcgt ccagtttctc tctgccgcgc agaccggcgg tcgccttcgc
    10141 gatcccgcgg tcgtacatct cccgctccca gacgccgaac gcgtcgagca gctcctcctt
    10201 ggaggcgaac cagcggtaga gggtcggacg ggatactccg gcctgtgcgg cgacctcgga
    10261 caggctgagt ttggtcatgc ctttgcggct gagcacctct gaggtggcga ccaggagccg
    10321 ctgacgcgtg gaggtgtcgt cgtcgacgtg atcgctgctt tctgctcttc gcgcaagcgg
    10381 ctcatcgctg ctttctgctc ttcgcgcaag cggctcatcg ctgctttctg ctcttcgcgc
    10441 aagcggctca tcgccgtcgc gtacgaccgc tgagcgtgtc atctctatag ctttacaaga
    10501 aactgacgaa gtgtcacgct catccggtgt ccgatccccg ttctcctggc gccgcgatag
    10561 aaagtgtggc atggccggac cgctagaagg catcaatgtc gtcgaactcg gcgtctgggt
    10621 cgccggcccg gccgcgggag gcatcctcgc ggactggggc gcagaggtcg tcaagatcga
    10681 gccgccgcgc ggagatccgg cgcggatgtt cggccggatg ctgg
//
LOCUS       DASWBF010000521         6612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_62810, whole
            genome shotgun sequence.
ACCESSION   DASWBF010000521 DASWBF010000000
VERSION     DASWBF010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62810"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1470
                     /locus_tag="VF874_19395"
     CDS             283..1470
                     /locus_tag="VF874_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9178141.1"
                     /translation="MRAFPVILPSGACYWTVLDEDLAVVAVADGFLRQVRFGRDGAES
                     TTKSYAHSIALFLRWCTRTGRTWQAGAEQLPLFTTWLAHAGPAVSSANTSSVVVSAGP
                     GSVPARGARRINGVLTAVRAMVVHAVSTGQADGNLLSVLYEVADDRDLPQAARSGDGP
                     MSWRMRARHRLHEPERPVDRASDVDIVALLGACRSARDRLIVLLMARAGLRRGELCGL
                     RRSDVHLLNDSRPLGCEITRAHVHVVRRGDNPNGAWAKSRRQRAVPLDFLTVQAFDTY
                     VFERMTVPRAAAGDFVVVNLFREPIGAPMRPDAIGELLAAASRRAGLTTAITPHRLRH
                     AFGSNAADAGCGIDVLADLLGHASVSSSQVYLHQPSGIASGGRVSAAGQVRGQGYVTG
                     LAV"
     gene            1633..2655
                     /locus_tag="VF874_19400"
     CDS             1633..2655
                     /locus_tag="VF874_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9178142.1"
                     /translation="MVEAMLRGWRAQQAARGLRESTAVDRERSVRRFLAFTNEYPWGW
                     TPTHVDEWSLWMASEKRLAPSTIRGYQGSLRLFSEFLSDARYGWIVACEREFGTHPVP
                     ICHEWNTIAHLNSYEGRPEARPFTREELQRFLDYADDQVERTLRAKRKGALAAYRDAT
                     LFKVVYGWGLRRTEAAKLDVVDFGRNPKAPQFGRFGMLNVRYGKAKKGQPPRRRNVLS
                     VMDWAVEAVADYVENVRPRFGFDDHPALWVTERGGRIKPAEINARFVAYRDALKLPKT
                     LVPHSIRHSYVTHLTEDGVDRRFIQSQVGHECDSSTAIYTHVSDDYMNTALRQALSPA
                     FAPSTP"
     gene            2668..3000
                     /locus_tag="VF874_19405"
     CDS             2668..3000
                     /locus_tag="VF874_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9178143.1"
                     /translation="MAPKLDYRWNLRRVMAERGMFQTTDLIDPLAKRGIELSSSQVYR
                     LVVERPERLSLKVLMALLDILDCAMDDLIEPVATVSAATKRKAVGADSSVGDLRPKRA
                     RIRGAGQS"
     gene            2997..5432
                     /locus_tag="VF874_19410"
     CDS             2997..5432
                     /locus_tag="VF874_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178144.1"
                     /translation="MTVVEQDRAIADPIGLITALVAAAEPHLALDRIRSVVVAVAGGR
                     AKSRRLAAALAARPGVLLDGRSPAPRVVGELLRALRAAGATAISPPSCATCGKALRTF
                     TRRGEHWYCGPCEQRRAPCAGCGEIKRVKVVDREGRPRCAQCADVDDRDPISVIHAVV
                     AELDPRVGYDTVADVVDRSCRQRAYQQKLAWAIESDPALLTGDAHRAPLRVIPRFVEH
                     LHAAQVVGVVLPTCPGCRRAVRIDKPLNGVRVCRTCIAHSRIEECSRCQAGREPVTRD
                     RHGRPICANCFVTDPTNLETCLCCGRLRPVSRRTIDGPLCQTCPALPVLTCSICGDTT
                     GCGISRTTGRPWCPACQRRSATCTGCGDHTSIAAGALARPLCADCAPPTAWLDCPTCS
                     DPDHPKPGQCARCRINRRLDEIMGPVTADLAPGLLALRHDIATAEHSITAHRWLTKEP
                     VVSVLSDLAAGRMPLTHEAFDELPKRQILEHLRLTLVGVGALPERDEELIRLERSLTD
                     FLDTQQDPVRRRLLHRYLTWHLLRRLRSRNNGRPTTRQQVLRIRAHRRAAEAFLDWLD
                     THNLTLTTFRQPDLDRWLTDPAAGYRYEAATFIRWAHANKLTTAYLPTRRWHGPVNPL
                     DDDHRWATTRKLLHDNTVAVEDRLAGLLLLLYAQGPSTISLLTIDQISITDDDVRISF
                     GRTPIRLPEPVDELARTVVAKRKGHATIGATAASRWLFPGGQPGRPISTERLKLRLNR
                     LDIRPNQARSTALFQLATEIPAAILARTLGISVNSAVRWQQISAGDWTSYAADVSRRP
                     TSQTRTSGGIQTV"
     gene            5725..6558
                     /locus_tag="VF874_19415"
     CDS             5725..6558
                     /locus_tag="VF874_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEX9178145.1"
                     /translation="MALQFEGKVAFITGAARGQGRSHAVRFAEEGADIIAMDLCEQIG
                     SVAYPMARPEDLDETVNLVEKTGRRIVAEQGDVRDFEKLKAIVAKGVSELGRIDFVLA
                     NAGILPLAGEQGQTILAFDDAVDVMLKGVYYTMEAALPALLEHGEGGAIVITSSTAGL
                     TGMHTRFSMRNHGGAGYIAAKHGVVGIMRAYANMLAEKNIRVSTVHPTGLASPMIVNQ
                     QIEQLLGDQPEYGVAFENLLPVPAIEASDISEAMVYLCGKSGRYITGITLPVDAGFAV
                     K"
BASE COUNT         1113 a         2179 c         2187 g         1133 t
ORIGIN      
        1 cgggtcgacc acgagaagtc gttctgtgtc ggtcttggaa ggggcgatct gcagtagcgg
       61 caccagttcg ccggtgctgg gcagtcggta ctgaatcagg ctgtggtggc tgagttcaag
      121 cagttcctcc acccggatgc cttctccggg ggtcaacaac ctgtcgcatg acgttgcttg
      181 aacgcgtcgt gcgcctgggt tccacggcgg gtctcccggg gtgtggtgct cgtggctggt
      241 agttgttcac tgagccgacc atgagatccg gaggatccaa ggatgcgagc ttttccggtg
      301 atattgccgt cgggtgcgtg ttactggacg gtgctggatg aggacctggc ggtggtagcg
      361 gtcgcggacg gtttcctgcg tcaggtccgt ttcggtcggg acggcgcgga gtcgacgacg
      421 aagagctacg cgcattcgat cgcgttgttc ctgcggtggt gtacccggac gggccggact
      481 tggcaggccg gcgcggaaca actaccgctg ttcacgacgt ggttggccca tgccggccca
      541 gccgtttcga gtgccaacac atcgtcggtg gtggtgtcgg ccgggccggg cagtgtcccg
      601 gcccgcgggg cccggcggat caacggggtg ttgaccgcgg tgcgggcgat ggtggtgcat
      661 gcggtgagta ccgggcaggc ggacgggaat ctgttgtcgg tgctgtacga ggtggccgac
      721 gaccgggacc tgccgcaggc cgcccggagc ggggacgggc cgatgtcgtg gcggatgcgg
      781 gcccggcatc ggttgcatga gccggagcgg ccggtggatc gggccagtga cgtcgatatc
      841 gtcgcgttgc tcggtgcgtg ccggtcggcg cgagatcggt tgatcgtttt gttgatggcc
      901 cgggccgggt tgcggcgcgg cgagttgtgc gggttgcgtc gcagtgatgt gcacctgctc
      961 aacgattctc gtccgttggg ttgcgagatc acccgcgcgc acgtgcatgt ggtccgccga
     1021 ggcgacaatc ccaatggggc gtgggcgaag tcccggcggc agcgggcggt gccgctggat
     1081 ttcctgaccg tgcaggcctt cgacacatac gtgttcgagc ggatgacggt tccgcgggcc
     1141 gcggcgggtg atttcgtggt ggtcaacctg ttccgtgaac cgatcggtgc gccgatgcga
     1201 ccggacgcga tcggtgagct gctggccgct gcgtcgcggc gggcggggct gacaacggcg
     1261 atcaccccgc accggttgcg gcacgccttc ggcagcaacg ccgccgacgc aggatgcggc
     1321 atcgatgtgc tcgccgattt gctcggacac gcctcggtgt cctcgtcgca ggtatatctg
     1381 caccaaccct cgggaattgc atcaggcggg agagtttcgg ctgctggcca ggtgcgaggc
     1441 cagggatacg ttaccgggtt ggctgtctga aagcggctgc tggccaggag atttgcgcaa
     1501 gtcatcccgc ctgatgcatg attcacccac ttttcggatg cgtgggaggt gggggtgcgg
     1561 caggtcgatc tggcgggagc ggctcatctg gagctggtgt ccggcgtggt tcatctgcac
     1621 ccgcaggacg gcatggtcga ggcgatgctg cgcggctggc gggctcaaca ggcagcgcga
     1681 gggctgcggg agagcacggc ggtcgacagg gagcggtcgg tgcgccggtt cctggcgttc
     1741 accaacgagt acccgtgggg gtggacaccg acccacgtgg acgagtggtc gttgtggatg
     1801 gccagcgaga aacgcctggc accgtccacg attcgtggct atcagggcag tctgcggctg
     1861 ttcagcgagt tcctctctga tgcccgctac ggctggattg tggcgtgcga gagggagttc
     1921 ggcacccatc cggtcccgat ctgccatgag tggaacacga tcgcgcacct gaacagctat
     1981 gaaggccgcc cggaggcgag accgttcaca cgggaggagt tgcagcggtt cctcgactac
     2041 gccgacgatc aggtcgagcg cacgctccgc gcgaaacgca aaggagcgct ggccgcctac
     2101 cgtgacgcta cgttgttcaa ggtcgtctac ggctgggggt tgcggcggac ggaggcggcg
     2161 aagctggatg tagtcgattt cggccggaac ccgaaagcgc cgcagttcgg gcgcttcggc
     2221 atgctgaatg tccgctacgg caaggcgaag aagggtcagc cgccgcggcg ccggaatgtg
     2281 ttgtcggtga tggattgggc ggtggaggcc gtcgcggact atgtcgagaa cgtgcggccg
     2341 aggttcgggt tcgatgatca tccggcgttg tgggtcaccg aacgtggggg ccgaatcaaa
     2401 ccggcggaga tcaacgcacg gttcgtcgcc taccgcgatg cattgaagtt gccgaaaacg
     2461 ctcgtgcccc actcgatccg gcactcgtac gtcacccacc tcaccgagga cggtgtcgat
     2521 cggcgcttca tccaatctca ggtcggccac gagtgcgaca gttccacggc aatctacacc
     2581 cacgtcagtg acgactacat gaacaccgct ctgcgacagg cgctttcgcc tgcgttcgcc
     2641 ccgtccaccc catagataag gatcgccatg gcaccgaagc tggactaccg ctggaatctg
     2701 cgccgggtga tggccgagcg cggcatgttc cagaccaccg acctcatcga ccccttggcg
     2761 aagcggggca tcgaactgtc ctcgagccag gtctaccggc tcgttgtcga gcgcccggaa
     2821 cgactgagcc tgaaggtgct catggcgctg ctggacatcc ttgactgcgc gatggacgac
     2881 ctgatcgagc cagtcgccac ggtctcggct gccaccaagc ggaaggccgt cggtgccgac
     2941 agcagcgtcg gcgatctgcg gccgaagcgg gcccgcatcc gtggagccgg tcaatcgtga
     3001 ccgtggtcga gcaagaccgg gcgatcgctg acccgatcgg tctgatcacg gccctggtcg
     3061 ccgcggccga accgcatctg gcgctcgacc ggatccgttc ggtggttgtc gcggtggcgg
     3121 gaggacgggc gaaatcacgg cggctggccg cggcgttggc ggcacgtcca ggggttctgc
     3181 tcgacggccg gtccccggca ccccgcgtgg tcggtgagct gttgcgggca ttgcgcgcgg
     3241 ccggcgccac tgcgatctca ccgccatcct gcgccacctg cggcaaggcc ctgcggacct
     3301 tcacccgccg aggcgagcac tggtattgcg gtccttgcga acaacgacgg gcaccgtgtg
     3361 ctggctgcgg tgagatcaaa cgggtaaagg tcgtcgaccg tgaggggcgt ccgcgatgcg
     3421 ctcagtgcgc ggacgtcgat gaccgtgacc cgatctcggt gatccacgcc gttgtcgccg
     3481 agcttgatcc ccgcgtcggc tacgacacgg tcgccgacgt ggtcgaccgg tcgtgccggc
     3541 aacgcgctta ccagcagaaa ctggcctggg ccatcgagtc cgatcccgcg ctgctcaccg
     3601 gtgacgctca tcgagcaccg ctgcgggtga tcccccggtt cgtcgagcat ctgcatgcgg
     3661 cgcaagtggt cggcgtcgtc ctgccgactt gccccggttg ccgtcgtgcg gttcgtatcg
     3721 acaagccact caacggagtt cgggtctgcc gaacctgcat cgcgcactcc cgtatcgaag
     3781 aatgttcacg ttgccaggcc ggtcgggagc cggtcacccg cgatcggcac ggccgtccga
     3841 tctgcgccaa ctgcttcgtc accgacccga caaatctgga gacctgcctc tgctgcgggc
     3901 gcctgcgccc ggtcagtcgc cgcaccatcg acgggccgct ctgccagact tgccccgcac
     3961 ttccagtgct gacctgctcg atttgcggcg acacgaccgg gtgcggcata tcgcgtacca
     4021 ccggccggcc gtggtgccca gcctgccaac gccgatcggc gacctgcacg ggctgcgggg
     4081 accatacgtc gatcgccgcc ggcgccctgg cccgtccact ctgcgccgac tgcgcaccgc
     4141 ccacagcttg gctggactgt ccgacctgca gcgaccccga ccatcccaaa ccggggcagt
     4201 gcgcccgatg ccggatcaac cggcgcctcg acgagatcat gggaccggtc acggcggacc
     4261 tcgcccctgg gctgctcgcg ctgcggcacg acatcgctac cgccgaacat tccatcaccg
     4321 cccaccgctg gctcacgaaa gagcccgtgg tctcggtcct gtccgacctt gcggccggac
     4381 gcatgccgct gacccatgag gcgttcgacg aactgccgaa acggcagatc ctcgagcacc
     4441 tacgcctcac gctggtcggc gtcggtgcgc tccccgaacg cgacgaggaa ctcatccgtc
     4501 ttgaacgctc gctcactgat ttccttgata cacaacagga tccggtccgc cgtagactgc
     4561 tgcaccgcta cctgacctgg cacctgctca gacgtctgcg cagccgaaac aacggccgcc
     4621 ccacaacccg acaacaagtc ctgcgcatcc gcgcccaccg acgcgcggcc gaggcattcc
     4681 tcgactggct cgacacccac aacctcaccc tgaccacgtt ccggcagccc gacctcgacc
     4741 gctggctgac cgatcccgcc gccggatacc gctacgaggc cgccacgttc atccggtggg
     4801 cgcacgccaa caagctcacc accgcctacc tccctactcg ccgctggcac ggcccggtca
     4861 acccgctcga cgatgaccac cgatgggcaa ccacccggaa actactgcac gacaacaccg
     4921 tcgcggtcga ggaccgtctc gctggcctgc tcttgctcct ctacgcgcaa ggcccgtcca
     4981 ccatcagcct gctcaccatc gaccagatct cgatcaccga cgacgacgtc cggatcagct
     5041 tcggccgcac accaatccga ctacccgaac ccgtcgacga gctcgcccgc accgtcgtcg
     5101 ccaagcgcaa gggccacgcc accatcggcg ccaccgcggc gtcgcgctgg ctgttccccg
     5161 gcggccagcc cggtcgcccc atcagcaccg agcggctcaa actccggctc aatcggctcg
     5221 acatccgccc gaaccaggcc cgcagcactg cgctgttcca gctcgccacc gagatccccg
     5281 cggccatcct cgccagaacg ctcggcatca gcgtcaactc cgccgtccgc tggcaacaga
     5341 tctccgccgg agactggacc agctacgccg ccgacgtcag ccgccgccct acctctcaaa
     5401 cacgaaccag cggaggcatt cagacagttt gaatatccga cgtcaccatt tcagatattc
     5461 cgcgacaacg taaagccgtt gaggcgggag cgttgtcgca acatcttgag acagcgggaa
     5521 cgtgatgaaa ggaacgcctt ggtgctcaag ctccccaagc cgcagctgtt tcgcgcgtcc
     5581 gactcggcct ttggcagccc ggaggccgcc agtgttgccg acctgtgcag tcaattggcg
     5641 caatcaggct ctagcgcatt gctgaatgca gatactcggc ttgcgtgccc tgaccggcac
     5701 ttcgtccaac aaataggagg gatcatggca ctgcagttcg agggcaaggt cgcgttcatc
     5761 actggagccg cacggggcca gggtcgctcg cacgccgttc gattcgccga agaaggcgcc
     5821 gacatcatcg cgatggatct ctgcgaacag atcggaagcg tggcttatcc aatggctcgg
     5881 ccggaggatc tcgacgaaac cgtcaatctc gtcgagaaaa ccgggcgccg catcgtcgcc
     5941 gaacagggcg acgtccgcga ctttgagaag ctcaaagcca ttgtggccaa aggtgtttcc
     6001 gaactcggca ggatcgactt cgtgctggcc aacgccggca tcctgccact cgccggtgag
     6061 cagggccaga cgattttggc gttcgacgac gcagtagacg tgatgctcaa aggggtgtac
     6121 tacaccatgg aggcggcgct gccggcgctg ctcgagcacg gcgagggcgg agcgatcgtc
     6181 atcaccagct cgaccgcggg acttacgggt atgcacaccc gcttcagcat gaggaatcac
     6241 ggtggggccg ggtacatcgc cgccaagcac ggcgtggtcg gcatcatgcg ggcctacgcc
     6301 aacatgctcg ccgagaagaa catccgggtg agtaccgtgc atccgaccgg gctggcgagc
     6361 ccgatgatcg tcaaccagca gatcgagcag ctattgggcg atcagcccga gtacggcgtg
     6421 gcgttcgaaa acctgctgcc agtgcccgcc atcgaagcaa gcgacatcag cgaggcgatg
     6481 gtgtacctct gcggcaagtc gggccgctac atcaccggca tcacgctgcc ggtcgacgcc
     6541 ggattcgcgg tcaagtagcg ctgccgtgcg tggctcggca ttagctgaca ggcccggcgc
     6601 atctcacccc gg
//
LOCUS       DASWBF010000522         3782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_118354,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000522 DASWBF010000000
VERSION     DASWBF010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118354"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VF874_19420"
     CDS             <1..400
                     /locus_tag="VF874_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis bifunctional protein
                     CbiET"
                     /protein_id="HEX9178146.1"
                     /translation="TRTGTDCTAVAFERDDARRDRIRRNVVACGVTVEVRGAAPDAFD
                     GAAHPSAIFVGGGLTQPGLLEACCDRLPTGGRLVANAVTAESEAVLAQWYSSLGGELR
                     RFQHYAGEPLGKFTGWRPAMPVTQWAVSKR"
     gene            397..1152
                     /gene="cobM"
                     /locus_tag="VF874_19425"
     CDS             397..1152
                     /gene="cobM"
                     /locus_tag="VF874_19425"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HEX9178147.1"
                     /translation="MTVYFIGAGPGAADLITVRGERLLQRCPVCLYAGSIMPDDLLAL
                     CPSDARVVDTGPLTLDQIVAELSAADSAGHDVARLHSGDPSLYSAVAEQCRRLETLGI
                     DYEIVPGVPAFAAAAAVLRRELTVPGVAQTVTLSRVATLSTAMPPGEDLATLSLSGAT
                     LVLHLAAAQIDAVVPELIAGGYGPETPCAVVAFATWPQQQVLYGTLAGIADQMHAAAI
                     TRTAVIIVGDVLAADGFTDSYLYSPARRRSGRH"
     gene            1152..1886
                     /locus_tag="VF874_19430"
     CDS             1152..1886
                     /locus_tag="VF874_19430"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HEX9178148.1"
                     /translation="MRVLLLGGTAEGRALAQRLHPDVEVISSLAGRVPDPALPVGQVR
                     IGGFGGAQPMRQWLVENRVDAVVDATHPFAAAMTERAARVCADLGLPHVVVARPPWDS
                     SDAIAVASAAEAAEVVEQQRYSRVFLTSGWSTISAFAHSSAWFLIRVVIAPNADALPT
                     RHTLLLSRGPYRYDDEIALMREHNIHVLVTKNSGGDYTRAKLDAAAALYIPVVMIDRP
                     PLPPGVTTVGTVDEAVAWVTRCRRDG"
     gene            1983..2216
                     /locus_tag="VF874_19435"
     CDS             1983..2216
                     /locus_tag="VF874_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178149.1"
                     /translation="MADRPGQRVVRLGILRSPPAEFQNHNGPSLAPSADGRLELFFVE
                     GGILWHIWQTAPSNGWSGWASHGKPPGFTFFGP"
     gene            complement(2227..3678)
                     /locus_tag="VF874_19440"
     CDS             complement(2227..3678)
                     /locus_tag="VF874_19440"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEX9178150.1"
                     /translation="MTGTLWGVGLGPGDPELVTVKAARVIGEADVVAYHSARHGRSIA
                     RGIAEPYLRAGQIEEHLVYPVTTETVDHPGGYAGAMEDFYRQCAEKIAAHLDAGRDVA
                     LLAEGDPLFYSSYMHMHTRLTERFDAVIVPGVTSVSAASAATATPLVQGDEVLAVLPG
                     TLPVAELAARLSDADAAVVLKLGRSYPAVREALSLAGRLDDAFYVERASTAAERVLPA
                     SSVDDASVPYFSLALVPGGARASSVGGVAVVGLGPGALDWMTPQSRRELAAATDLIGY
                     GPYLERVPARDGQRRHPSDNTDEPARARLACTLAEQGRAVAVVSSGDPGVFAMATAVL
                     EEAKQWPGVRVRVIPAMTAAQAVASRVGAPLGHDYAVLSLSDRLKPWDVIEARLIAAA
                     QADLVLAIYNPASKSRTWQVAAMRDVLLRHRDPGTPVVIGRDVSGPDEDVRVVRLADL
                     DPDSVDMRCLLIVGSSQTQWYDDTVFTPRRYPS"
     gene            complement(3675..>3782)
                     /locus_tag="VF874_19445"
     CDS             complement(3675..>3782)
                     /locus_tag="VF874_19445"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX9178151.1"
                     /translation="LVARPRGIAYLLVTGRRGGSAMAAAAVNAMAGGPA"
BASE COUNT          553 a         1357 c         1314 g          558 t
ORIGIN      
        1 gacgcgcacc gggactgatt gcacggccgt cgcattcgag cgcgacgacg cgcgccgtga
       61 ccggatccgg cgcaacgtcg tcgcgtgcgg cgtcacggtc gaggtccgcg gcgccgcgcc
      121 ggatgcgttc gacggggccg cacacccatc ggcgatcttc gtcggcggcg ggctcacgca
      181 gccggggctg ctggaggcct gctgcgaccg gctgccgacc gggggccggc tcgtcgccaa
      241 tgccgtcact gctgaatcgg aagctgttct tgcgcaatgg tattcgagcc tgggcggtga
      301 actgcgacgg tttcagcact acgccggcga gccgctgggc aagttcaccg gctggcgccc
      361 agcgatgccg gtcacccagt gggcggtgag caagaggtga ccgtctactt catcggcgcg
      421 gggccgggtg ccgcggatct gatcaccgta cggggtgagc ggctgctgca gcgctgcccg
      481 gtgtgcctct acgcggggtc gatcatgccc gacgacctgc tcgcgctgtg cccgtccgac
      541 gcccgcgtgg tcgacactgg gccgctgaca ctggaccaga tcgtcgccga actgtcggcc
      601 gcggactccg ccggccacga cgtcgcccgt ctgcactcgg gggacccgtc gctgtacagt
      661 gcggtagccg aacagtgccg ccgcctggag acgctcggca tcgactacga aatcgtgcct
      721 ggcgtaccgg ctttcgcagc cgctgccgcg gtgctacgcc gcgagctgac ggtgcccggg
      781 gtggcgcaaa ccgtcacgct gagccgggtg gcgacgctgt cgacggcgat gccgccgggc
      841 gaggacctcg ccacgctgtc gttgtccggc gcgacacttg tgctgcacct ggcggccgcg
      901 cagatcgacg ccgtcgtgcc agaactgatc gcggggggat atgggcccga aacgccttgt
      961 gcggtcgtcg ctttcgcgac gtggccacag cagcaggtgc tatacggcac actcgccggc
     1021 atcgccgatc agatgcacgc cgccgcaatc acgcggaccg cagtgatcat cgtcggcgac
     1081 gtgctcgccg ccgacgggtt cacggacagc tatctgtatt cgccggcccg gcgacgcagc
     1141 gggcggcact gatgcgggta ctgctgctcg gcgggacggc ggaggggcgc gcactcgcgc
     1201 agcggctgca ccccgacgtc gaggtgatca gttcgctggc cgggcgggtg cccgatcccg
     1261 cactgcctgt cgggcaggtg cggatcggcg ggttcggcgg cgcccagccg atgcggcagt
     1321 ggctggtcga gaaccgggtg gacgccgtcg tcgacgccac gcacccgttc gccgcggcga
     1381 tgaccgaacg agcggctcgg gtgtgcgccg accttggctt gccgcacgtg gtcgtcgccc
     1441 gtccgccgtg ggactccagt gatgccatcg cggtggcgtc cgcggcggaa gccgctgaag
     1501 tcgttgagca gcagcggtat tcgcgtgtgt tcttgacgag cgggtggtcg accatcagtg
     1561 cgttcgcaca ctccagcgcc tggttcttga tccgcgtggt catcgcaccg aatgccgacg
     1621 cgctgcccac gcgtcacacc ctgctgttgt ctcgcgggcc gtaccgctac gacgacgaaa
     1681 tcgctctgat gcgtgagcac aacatccacg tgctcgtcac gaagaacagc ggcggcgact
     1741 acacccgcgc caagctggac gccgccgcgg cgctttacat tcccgtcgtg atgatcgacc
     1801 gtccgccgct tccgccgggc gtcacaacgg ttggaaccgt cgacgaggcg gtcgcctggg
     1861 tgacacgatg tcggcgtgac ggctaacgct cccacacgat ggcttgcccg cagcaaggac
     1921 gggcggttgg aacttttcga tgtgggcgtc ggtagcgacg cccacatcga gttgtggcac
     1981 atgtggcaga ccgccccggc cagcgggtgg tcaggctggg catcttaagg tctccacccg
     2041 cagagttcca gaatcacaac ggcccgtcgc tggcgccaag cgctgacggc aggctggagc
     2101 tgttcttcgt agagggcgga atcctctggc acatctggca aaccgcgccc agcaacggct
     2161 ggtcgggctg ggcgtctcac ggtaaaccac cgggcttcac gttcttcggg ccttgagccg
     2221 gcccgactac gacgggtagc ggcgcggagt gaacaccgtg tcgtcgtacc actgcgtctg
     2281 cgacgagccg acgatcagca ggcagcgcat gtcgacgctg tccggatcca ggtcggccag
     2341 ccgcaccacc cgcacgtcct cgtccgggcc ggaaacgtcg cgcccgatca ccaccggagt
     2401 gcccgggtcg cggtgccgca gcagcacatc gcgcatcgcc gcgacctgcc aggtgcggct
     2461 cttcgacgcc gggttgtaga tcgcgagcac caggtcggcc tgcgcagccg cgatcagcct
     2521 cgcctcgatt acgtcccacg gcttgagccg gtcggacagc gaaagaacgg catagtcgtg
     2581 gccgagcggc gcgccgaccc ggctggccac cgcctgcgcc gccgtcatcg ccgggatcac
     2641 ccgaacccgc accccgggcc actgtttggc ctcttcgagc accgcggtcg ccatcgcgaa
     2701 cacgcccgga tcgcccgacg acaccaccgc gaccgcgcgg ccctgctcgg ccagcgtgca
     2761 ggccagccgc gcgcgcgccg gctcgtcggt gttgtcactg ggatggcggc gttgcccgtc
     2821 gcgtgccggc actcgctcca gatacggccc gtacccgatc aggtcggtgg ccgccgccag
     2881 ctcgcgccgg ctctgcggcg tcatccagtc cagcgcgccc gggcccagcc caaccacggc
     2941 gacgccgccc actgaggatg ctcgcgcacc gccgggcacc aacgccaacg agaagtacgg
     3001 cacggacgcg tcgtcgacgg acgacgccgg cagcacccgc tcggccgccg tgctggcccg
     3061 ctcgacgtag aacgcgtcgt cgagccggcc cgcaagcgaa agcgcttcgc gcacagcggg
     3121 atacgagcgc cccaacttca gcaccacggc cgcgtcggca tccgacagcc gcgcagccag
     3181 ctcggccacc ggcaaggtgc cgggcagcac cgccagcact tcgtcgcctt gcacgagtgg
     3241 ggtggcggtc gcagccgacg cggcgctcac cgacgtcacg ccgggcacga tcaccgcgtc
     3301 gaaccgttcg gtgagccgag tgtgcatgtg catgtaggag ctgtagaaca gcggatcgcc
     3361 ctcggcgagc agcgccacgt cgcggccggc gtccagatgc gcggcgatct tttcggcgca
     3421 ctgccggtag aagtcctcca tcgcgccggc gtagccgccc ggatggtcga cggtctcggt
     3481 ggtcaccggg tagaccaggt gctcctcgat ctggcccgcc cgaaggtacg gctcggcgat
     3541 gccgcgcgcg atgctgcggc cgtgccgcgc gctgtgatac gcgaccacgt ccgcctcgcc
     3601 gatgacgcgg gcggccttga cggtgacgag ctcgggatcg ccgggcccca gcccgacgcc
     3661 ccacagcgtg cccgtcacgc cgggccgccg gccatcgcgt tcaccgcggc ggcagccatc
     3721 gcactgccgc cccgccgtcc tgtcaccagc agatacgcga tgccgcgcgg gcgggcgacg
     3781 ag
//
LOCUS       DASWBF010000523         7661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1312599,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000523 DASWBF010000000
VERSION     DASWBF010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312599"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..560)
                     /gene="mscL"
                     /locus_tag="VF874_19450"
     CDS             complement(96..560)
                     /gene="mscL"
                     /locus_tag="VF874_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX9178152.1"
                     /translation="MLKGFKEFLARGNIVDLAVAVVIGTAFTALVTAFTKSVIEPLIN
                     RIGAGPDAQYGILRFDIGGRQAIDLNVVLTQAINFLLVAAVVYFLVVLPYNTFRKRGE
                     VEQAQDTELSILTEIRDLLATDGQASGRHETVTGPGTGPSPDTVKTTSADRT"
     gene            complement(618..1283)
                     /locus_tag="VF874_19455"
     CDS             complement(618..1283)
                     /locus_tag="VF874_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEX9178153.1"
                     /translation="MGESLNPSALSRLSRLLRPDWSRTLAARRVAAAGLVVLAAVAAF
                     RSDPHGDRADAVVATRDLAPGVSLTADDVRVEKRLATTIPDGARAGVDEVVGATLAGP
                     ARRGEVLTDVRLLGPRLATSAAGPDARIVPLHLADSALLELLRAGDVVDVLAAGPPNL
                     SSDVESAPHVVATDAVVVLVSPKPAQRGPGNERVVLVALPAATAHRVAGVALVETVTL
                     TFH"
     gene            complement(1348..1674)
                     /locus_tag="VF874_19460"
     CDS             complement(1348..1674)
                     /locus_tag="VF874_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX9178154.1"
                     /translation="MPTYSYACTECDNRFDAVQAFTDQALTACPQCSGRLRKLFGKVG
                     VVFKGSGFYRTDSRAASGSSNGEKKATADSSSASSPSSDTSSTSKTNGEAKSSSATPA
                     PAAAAS"
     gene            complement(1758..2342)
                     /locus_tag="VF874_19465"
     CDS             complement(1758..2342)
                     /locus_tag="VF874_19465"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX9178155.1"
                     /translation="MATTLKDRIRADRVAARRAVGEDLRRTETQALVRHLHAMLDPDD
                     VVAAYAPLPAEPGFPDLADAVVAICGTVLLPIARPSSDGIPLPLQWSAYRYGALVAAP
                     YRLFEPPPPWLPPSALGSVGTILVPALAVDRHGVRLGRGAGFYDRSLGFRDPAARLIA
                     VVRDDELVDELPVEPHDVAMTHALTPGSGLVSLI"
     gene            2415..3326
                     /locus_tag="VF874_19470"
     CDS             2415..3326
                     /locus_tag="VF874_19470"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX9178156.1"
                     /translation="MTRPEIPIPRTAIVPAAGLGTRFLPATKTVPKELLPVVDTPGIE
                     LVAGEAADAGAERLVIVTSEGKDSVVAHFVEDLVLEGTLEARGKYSMLEKVRRAPALI
                     KVESVVQAQPLGLGHAVSCVEPVLSDDEDAVSVLLPDDLVLPTGVLETMSKVRAKRGG
                     TVLCAIEVPPSEISAYGVFDVETVPDAANPNVLRVKGMVEKPTAEDAPSLYAAAGRYV
                     LDRAIFDALRRVPRGAGGEIQLTDAIALLIDEGHPVHVVVHRGSRHDLGNPGGYLKAA
                     VDFALDRDDYGPDLRRWLVARLGLPEQ"
     gene            3367..4632
                     /gene="glp"
                     /locus_tag="VF874_19475"
     CDS             3367..4632
                     /gene="glp"
                     /locus_tag="VF874_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX9178157.1"
                     /translation="MRSVEEQQARVMAAAVAPRPVRVAIAEAQGLMCAEEVVTERPLP
                     GFDQAAIDGYAVRSVDIAEVGEGNDSDDLAEVGGIEVRLPVVGEIEAGARTPSRLQPR
                     QAARVRTGAPMPTLADAVLPLRWTDGGESKVRVLRSVRRGAYVRHAGDDVQPGDVAVR
                     AGAIIGAAQVGLLAAVGRERVLVHPRPRLSVLSVGSELVDISRTPGNGQVYDVNSYAL
                     AAAGRDAGAEVTRVGIVSTDPKELREVVEGHLNRAEVVVIAGAVGGAAAEDVRSALSA
                     LGEMDITRVAMHPGSVQGFGQLGAEGVPTFLLPANPVSALVVFEIMVRPLIRLSLGKR
                     QPMRRIVEARTLSPISSVPGRRRYLRGQLMRDQDTDDYLVQVLGAPGASSHLLATLAE
                     ANCLVIVPSDAEQIRTGEIVSAAFLAQRG"
     gene            4652..5305
                     /locus_tag="VF874_19480"
     CDS             4652..5305
                     /locus_tag="VF874_19480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX9178158.1"
                     /translation="MGFFRNSSAHPGWPATVGPIRVAGGVVRLRPVRMRDAAQWSRIR
                     LADREHLEPWEPTTDTDWVRRQTVSSWPSVCSGLRSEARKGRMVPFAIELDGEFCGQL
                     TVGNVTHGALRSAWIGYWVASSATGKGVATGALALGLDHCFGAVGLHRVEATVRPENA
                     ASRRVLAKVGFREEGLLQRYLDVDRAWRDHLLVAITVEEVRGSVAGTLVREGQAHWS"
     gene            5535..6491
                     /gene="glpR"
                     /locus_tag="VF874_19485"
     CDS             5535..6491
                     /gene="glpR"
                     /locus_tag="VF874_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase receptor GlpR"
                     /protein_id="HEX9178159.1"
                     /translation="MPSVPQSLLWISLVVLWLFVLVPMLISKRDAVRRTSDVALATRV
                     LNSGVSTRLRKRRPADGHRSDPGWKPVDEDDDLDDEPSSQAVVVKAAQPAPEQDIIDV
                     DVVEEDSGALPVGASEPAEPEQEPETEAEPEPVSADEQPADSDDDGTSDEYEYVEDTS
                     GLESEEDPPARKTPPITSRRRLYESKTAATVSARKYRFRKRLLVAMSLILIATTLAAV
                     TVWPAGWWVCGAAGTITILYLAYLRRQTRIEERVRRRRTQRMARARVGVHNTADRDYD
                     MPSRLREPGAAVLEIDDEDPIFEHLDYAPGPMEYHLPKAAGQ"
     gene            6544..6616
                     /locus_tag="VF874_19490"
     tRNA            6544..6616
                     /locus_tag="VF874_19490"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6577..6579,aa:Ala,seq:cgc)
     gene            6694..>7661
                     /locus_tag="VF874_19495"
     CDS             6694..>7661
                     /locus_tag="VF874_19495"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9178160.1"
                     /translation="MASIHAQKNRTGNTYRVLWRADGRQRSLTFENLPSAERFKTLLE
                     DHGPDEALRVIELDEIGRHVPTVTEWLHTHIDNLTGVQPATLARYRSYVTRDIDPVFG
                     SMPVSAVTETTIARWVQQLGGSGKTISNKHGFLSGAFNAAVRARVMASNPCQGRRLPH
                     TRVAETVFLTPPEFVLLHDHIERDRWKNLATWLVTTGMRFSEATALTAADIDAEARTC
                     RITKAWKYSGDYRPAIGPPKTRKSIRTISLPPAALEVVDLTAPGFVFTNGAGNPVRAQ
                     EFFNGGWKPGRDAAMKAGLTKCPRVHDLRHSCASWMIAAGVPLPII"
BASE COUNT         1243 a         2453 c         2765 g         1200 t
ORIGIN      
        1 gagcccggac accgtgaaga cgacgagcgc ggacaggacg tagtcgccaa cgaatcagcc
       61 cccggccgat cggccggggg ctgattcgtt ggcgactacg tcctgtccgc gctcgtcgtc
      121 ttcacggtgt ccgggctcgg accggttccc ggcccggtca ccgtctcgtg ccggcccgag
      181 gcctgaccgt cggtggccaa caggtcgcgg atttcggtca agatgctcaa ctcggtgtcc
      241 tgcgcctgct ccacctcgcc gcgcttacgg aacgtgttgt acggcaggac caccaggaag
      301 tacaccaccg cggcgacgag caagaagttg atggcctgcg tcagcacgac gttgaggtcg
      361 atggcctggc ggccgccgat gtcgaatctc aggattccgt attgcgcatc agggcccgcg
      421 ccgatgcgat tgatcagcgg ttcgatgacg ctcttggtga acgccgtgac cagcgcagtg
      481 aacgcggtgc cgatgacgac cgcgacggcg aggtcgacga tgttcccccg tgccagaaac
      541 tccttgaatc ccttcagcat gtagcggtcc tcctgtgctc ggtcagcggt cgtcgtgagc
      601 cgtacagtag tcgccgctca gtgaaaggtc agcgtcaccg tctccaccag cgccacgccg
      661 gccacccggt gagcggtggc cgcgggcagc gccaccagaa ccacccgttc gttgcccggc
      721 ccgcgctgcg cgggcttggg ggacaccagc accacgaccg cgtcggtggc taccacgtgc
      781 ggggcggact cgacgtcgct cgacaagttc ggcggtccgg cggccagcac gtcgacgacg
      841 tcgccggcgc gaagcaactc cagcagcgcg gagtcggcca gatgcaacgg cacgatgcgg
      901 gcatccgggc cggcggccga tgtggccaac cgcgggccga gcagccggac gtcggtgagc
      961 acctcgccgc gcctggccgg tccggcgagc gtcgcgccga cgacttcgtc gacgccagct
     1021 cgtgcgccat cgggaatcgt tgtggccaaa cgcttttcga ctcgcacgtc gtcggcggtc
     1081 agcgagacac cgggcgccag atcccgggtt gccaccaccg cgtcggcgcg gtcgccgtgc
     1141 ggatcggacc gaaacgcggc gacggccgct aacaccacca accccgccgc ggcgactcgc
     1201 cgcgccgcca gggtgcgcga ccagtccggt cgcagtaggc gcgaaaggcg actcagcgca
     1261 gacggattga gcgattcccc cattcggcca cgttaagcgg acgagggcag gcgcggaggg
     1321 ccaagtcgag gccctgtgga taacggatca gctggcggcg gcggcaggcg ccggggtagc
     1381 actggatgac ttcgcctcgc cgttggtctt cgacgtcgac gaggtgtcgc tcgacgggct
     1441 ggacgcgctg gacgaatcgg cggtggcctt cttctcgccg ttggacgagc cagaggccgc
     1501 ccggctgtcg gtgcggtaga agccgctgcc cttgaagacg acgccgacct tgccgaacag
     1561 tttgcgcagc cggcccgagc actgcgggca tgcggtcagg gcctggtcgg tgaatgcctg
     1621 caccgcgtcg aaccggttgt cacactcggt gcacgcatag gagtaggtcg gcaagggaac
     1681 ctccggttag gtcagctttc tagcactcta cctattcaag tgccaacgag ccagcttgca
     1741 ttcccggaat ccggtgctta tatcagcgaa accagcccgg atccgggggt cagggcatgg
     1801 gtcatcgcga cgtcgtgtgg ctcgaccgga agctcgtcca cgagttcgtc gtcacgcacg
     1861 acggcgatca gccgggcggc agggtcgcgg aaccccagcg agcggtcgta gaacccggcg
     1921 ccacggccca ggcggacacc gtggcggtcg acggccaagg ccggaaccaa aatcgtgcca
     1981 accgagccca gcgccgaggg cggcaaccac ggcgggggcg gctcgaacag cctgtatggc
     2041 gctgcgacca gcgcgccata ccggtaggcg gaccactgca acggcagcgg gatgccgtcg
     2101 gacgacgggc gggcgatcgg cagcaacact gtcccgcaga tggcgaccac ggcgtcggcg
     2161 aggtctggga agcccggttc ggccggcagc ggcgcgtagg cggcaaccac gtcgtcaggg
     2221 tcgagcatgg catgcagatg ccgaaccagc gcctgggttt ctgtgcggcg cagatcctca
     2281 ccgacggctc gccgcgcggc gacacggtca gcgcggatac ggtcctttaa cgttgtcgcc
     2341 acggggtaac gatgacaggg ggcccataac gatgccacaa gccactcaca agccgcggcg
     2401 ataaggtggg agcgatgacc cgaccagaaa taccgattcc gcggactgcg atcgtgccgg
     2461 ccgcgggtct gggcacgagg ttcctgccgg ccaccaagac ggtccccaag gagctgctgc
     2521 ccgtcgtcga cacgcccggc atcgagctcg tcgcgggtga ggccgccgac gcaggtgccg
     2581 aacggctcgt catcgtgacc tctgaaggca aagacagtgt ggtcgcccac ttcgtcgagg
     2641 acttggtgct cgagggcacg ctggaggccc gcggaaagta ctcgatgctc gaaaaggtgc
     2701 ggcgcgcacc ggctttgatc aaggtcgaat cggtggtgca agcccagccg ctggggctgg
     2761 gtcacgccgt cagctgcgtg gagccggtgc tgtccgacga cgaggacgcg gtctccgtgc
     2821 tgctgcccga cgaccttgtg ctgcccaccg gcgtgctgga aacgatgtcg aaggtgcgcg
     2881 cgaagcgggg cggcaccgtg ctgtgcgcga tcgaggtgcc gccgtcggag atcagcgcct
     2941 acggcgtgtt cgacgtcgag acggtgcctg acgcggccaa cccgaacgtg ctgcgcgtca
     3001 agggcatggt cgaaaagccc acggccgagg atgcgccgtc gctctatgcc gccgccggcc
     3061 gctacgtgct ggaccgggcg atcttcgacg cgctgcgtcg tgttccgcgc ggagcgggcg
     3121 gcgagatcca gttgaccgac gcgatcgcgc tgctgatcga cgagggacat ccggtgcacg
     3181 tggtggtgca tcgcgggtcc cgacacgact tgggaaatcc cggcggctac ctgaaggctg
     3241 cggttgactt tgcattggat cgtgatgact atggcccgga cttgcggcgc tggctggtag
     3301 cgcgattggg cttgccggag cagtagtcac gtgccgggcc cggctctgac ggcagaaagg
     3361 catgctgtgc gttcggtgga ggagcagcag gcacgcgtga tggcggcggc ggtcgcgcct
     3421 cgaccggtcc gcgtcgcgat cgccgaagcc cagggcctga tgtgcgccga ggaggtggtc
     3481 accgagcggc cgctgcccgg gttcgaccag gcggcgattg acggttacgc ggtgcgcagc
     3541 gtcgacatcg ccgaagtcgg tgagggcaac gactccgacg accttgccga agtcggcggc
     3601 atcgaggtgc ggctaccggt cgtcggcgag atcgaggccg gcgcgcggac accgagccgg
     3661 ttgcagccgc ggcaggccgc ccgcgtgcga acgggcgcgc cgatgccgac gctcgccgac
     3721 gcggtgttgc cgttgcgttg gaccgacggc ggcgagtcga aggtgcgggt gttgcgcagc
     3781 gtgcgccggg gtgcctacgt gcgacacgcc ggtgacgacg tgcagcccgg cgacgtcgcg
     3841 gtgcgggccg gggcgatcat cggcgcggcg caggtcggct tgctcgcggc ggtcggccgg
     3901 gagcgggtgc tggtgcatcc gcggccgcgg ttgtcggtgc tgagcgtcgg cagcgagctc
     3961 gtcgacatct cccgcacgcc gggcaacggg caggtgtacg acgtgaactc gtacgcgttg
     4021 gcggcggcgg gccgtgacgc gggtgcggag gtgacgcgcg tcgggatcgt cagcaccgac
     4081 ccgaaggaac tccgcgaggt cgtcgaaggc cacctcaacc gcgccgaggt ggtggtgatc
     4141 gccggggcgg tcggtggggc cgccgcggaa gacgtgcggt cggcgttgtc tgcgctcggc
     4201 gaaatggaca tcacccgggt cgcaatgcat cccggctcgg tgcagggatt cggtcaactc
     4261 ggtgccgagg gggtgccgac gttcttgctg cccgccaacc cggtgagcgc gctggtggtg
     4321 ttcgaaatca tggtccgccc gctgatccgg ctgtcgttgg gcaagcgtca gccgatgcgg
     4381 cgcatcgtgg aagcccgtac gctgtcgccg atttcgtcgg tgcccggccg ccggaggtat
     4441 ctgcggggac agttgatgcg cgatcaggac accgatgact acctggtgca agtgctcggc
     4501 gcgccgggcg cgtcgtcgca cttgctggcg accctggccg aggccaactg cctggtgatc
     4561 gtcccgagcg acgccgaaca gattcgcacg ggggagatcg tcagcgccgc gttcctggcc
     4621 cagcgcggct gaaacgcgaa acccgttcgc tgtgggcttt tttcgtaaca gttccgctca
     4681 cccgggttgg ccggccacgg tgggtccgat cagggtggcc ggcggggtgg tgcggctgcg
     4741 cccggtccgg atgcgcgacg cggcgcagtg gagccggatt cggctggccg accgtgagca
     4801 tctggagccg tgggagccga cgaccgacac ggattgggtt cgcagacaaa cggtttcgtc
     4861 gtggccgtcg gtgtgctcgg gattgcgttc ggaggcgcgc aaggggcgca tggtgccgtt
     4921 cgccatcgaa ctggacggcg agttctgcgg gcagctgacg gtcgggaacg tcacgcacgg
     4981 cgcgctgcgc tcggcatgga tcgggtactg ggtagccagc tcggccactg gcaagggggt
     5041 ggcgacgggc gcgctggcgc tcgggctgga ccactgcttc ggggcggtgg ggttgcatcg
     5101 ggtggaggcg acggtgaggc ccgagaacgc cgcgagccga cgggtgctgg ccaaggtcgg
     5161 cttccgcgag gagggcctgt tacaacgcta tctcgacgtc gaccgagcgt ggcgcgacca
     5221 cctgctggtc gcgatcaccg tcgaagaagt gcgcggctcg gttgcgggca cgcttgtgcg
     5281 cgagggccag gcgcactggt cctgaccggc cgtctgcgcc gaaaccgaat tccaggtcgt
     5341 cgttcggccg cgaacgcgac catggcttca ttctcggcgc gcggcgtcga ccaatcagtt
     5401 acagatgtga cacgtgtgcc tggtggtgct tgcctgaaac gaattacagg tgtgtaattg
     5461 tgccggtcac gcggcgcgcc ttcgcccttt gcgctgatca cggacctagc ctgacggtga
     5521 aaggagcagg caccatgcca agcgtcccgc aatcactcct gtggatctcc ctcgtggtgc
     5581 tgtggctgtt cgtcctcgtg ccgatgctga tcagcaagcg tgacgccgtg cgacgcacca
     5641 gcgacgtcgc gctggccacc cgggtgctga acagtggcgt cagcacccgg ttgagaaaac
     5701 ggcgaccggc cgacgggcac cgcagcgatc cgggctggaa gccggtcgac gaggacgacg
     5761 atctcgacga cgaaccgtcg tcgcaggcgg tcgtcgtgaa ggcggcccag cccgcgcccg
     5821 agcaggacat catcgacgtc gacgtcgtcg aagaggattc cggggcgctg ccggtcggcg
     5881 ccagtgagcc cgccgagccg gagcaggaac cggagaccga agccgagccg gagcccgtct
     5941 ccgccgacga gcagccggcc gactccgacg acgacggcac ctccgacgag tacgaatacg
     6001 tcgaagacac ctcgggattg gagtcggagg aggatccgcc ggccaggaag acaccgccca
     6061 ttacgtcgcg gcgtcggcta tacgaatcca agaccgcggc gacggtcagc gcccgcaagt
     6121 accgcttccg gaagcgtctg ctggtggcga tgtcgctgat cctgatcgcg acgaccctcg
     6181 cggcggtcac cgtgtggccc gctgggtggt gggtgtgcgg cgccgccggg acgatcacga
     6241 tcctgtacct ggcttatctc cgccggcaga cccgcatcga ggaacgggta cgacggcggc
     6301 ggacccagcg gatggcgcgc gcgcgggtcg gtgtgcacaa caccgctgat cgcgattacg
     6361 acatgccgtc gcggctgcgc gagcctggtg cggcggtgtt ggaaatcgac gacgaggacc
     6421 caattttcga gcatctcgat tacgcgccgg ggccgatgga gtaccacctg ccgaaggccg
     6481 cagggcagta gcttcgctgg gttttcgggc cgggcccgtc ggctggtaac ctgctagcga
     6541 ccaggggcta tggcgcagtt ggtagcgcga ctcgttcgca tcgagtaggt caggggttcg
     6601 aatcccctta gctccactgg gaaaccgcag gtcagcggtg ccatctgaag tggcttccag
     6661 cgttacgtaa cagacggcgt aacattgggg gttgtggcaa gcatccacgc tcagaagaac
     6721 cggaccggca acacctaccg ggtgctgtgg cgtgctgatg gcagacagcg ctcgttgacc
     6781 ttcgagaacc tgccgtcggc ggagcgtttc aagaccctgc tggaggacca cggcccggac
     6841 gaggcgctgc gcgtcatcga gctcgacgag atcgggcgcc acgttccgac tgtgacggaa
     6901 tggcttcaca cccacatcga caacctcacc ggtgtgcagc cggcgaccct cgcgcgctac
     6961 cgcagctacg tcacccgcga catcgacccc gtgttcggtt ccatgccggt gtctgcggtc
     7021 accgagacca cgatcgcgcg gtgggtccaa cagctcggcg gcagcggcaa gacgatctcc
     7081 aacaagcacg gtttcctgtc gggggcgttc aacgcggcgg tgcgcgcgcg agtgatggcg
     7141 tccaacccgt gtcagggtcg tcgcttgccg cacacccggg tggcggagac cgtgtttttg
     7201 acaccgccgg agttcgtttt gctgcacgac cacatcgagc gggaccggtg gaagaacctg
     7261 gcgacgtggc tggtgaccac tggcatgcgg ttctcggagg ccactgcact gacggcggcc
     7321 gacatcgacg ccgaggcacg aacctgccga atcaccaagg cctggaaata ttccggcgat
     7381 taccggccgg cgatcgggcc acccaagacc cgcaaatcga tccgcacgat cagcctgccg
     7441 cctgcagcct tggaggtcgt cgacctgacg gcgcccgggt tcgtgttcac caacggtgcg
     7501 ggcaatcccg ttcgcgccca ggagttcttc aacgggggct ggaaaccggg ccgtgatgct
     7561 gccatgaagg cgggcctgac gaagtgcccg cgcgtgcacg atctgcgtca tagctgtgcg
     7621 tcgtggatga tcgctgccgg tgttccgttg ccgatcatcc a
//
LOCUS       DASWBF010000524         5860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1039320,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000524 DASWBF010000000
VERSION     DASWBF010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039320"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VF874_19500"
     CDS             <1..1048
                     /locus_tag="VF874_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HEX9178161.1"
                     /translation="AGDQRSLAIGMAGRVMELFFNARRRDASRLAAEHVELLESIGDP
                     MLTLALLPTVISAKHETAEMSDVLRLAQRGIDLAGGDATKGGELATSSPLTMALAYRG
                     LARWGFGITGWRENFSQAIALAQTGEAVLRGGAMYYTHVVAVMHGVVLPSAAAVRKAT
                     ETLALAEQTGEDVALGSARSNLGYFLVCVGGDSRDRGLELLALARELTMRRRYAMSGL
                     PIMDVMAARVHTELGNLDTAIDLSRAALDEVLEGGGTLWAPVAANVLVEALVTRGANG
                     DLDHAQTAVDRLAAVPIEPGLVLYDIWLLRLRALLAHARGDDVSYRGFADRYRKMATD
                     LGFEGHMAMAEAMR"
     gene            complement(1058..2134)
                     /locus_tag="VF874_19505"
     CDS             complement(1058..2134)
                     /locus_tag="VF874_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX9178162.1"
                     /translation="MASALVAALTAPLHELQARVGSGVFTGDDPARMLLDASRVLTDV
                     AASGAAATGQVGEMWSGLAADAATRTISTALSSAADLATHASGMASRVGAASEAVARA
                     KAQLQRIVDDFETTAYRLERDLPASAGSLADATRRALTDAAAVVAELEAELDGHAAAV
                     TESGAAVMPAAPVTSPAGLSPASGWYPQSSMSPAVAGVPSTLMGLTSGVLPGAGFAEA
                     PMLDSDWPGSGVAVRLPDGSTVKAPNRVAADAVRHALTQLGVPYDWGGTTPGVGLDCS
                     GLTQWAYDEAGLNLPRLAQEQDVGAAVSPNALLPGDLAVWDGHVAMVVGNGMMIEAGD
                     PVQLSPIRTTNLNQGFQGFFRPTA"
     gene            complement(2138..2440)
                     /locus_tag="VF874_19510"
     CDS             complement(2138..2440)
                     /locus_tag="VF874_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178163.1"
                     /translation="MHADTNSIRGFSGSTIDLGTDIRAAAAALSRDLGPSVTDALGPV
                     GARFAHALGDAAEALMTSVNKLGDEVLSHGSATAAAAGQYDEAELRAQARVANLTV"
     gene            2524..3129
                     /locus_tag="VF874_19515"
     CDS             2524..3129
                     /locus_tag="VF874_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX9178164.1"
                     /translation="MKWAAAALALLLCALTACGGADKSANYTLDSTTPGPETATTTTM
                     TSPPPPVSAPFALSSPAFADNTPIPAEYSCNGRNVPPPLRWENVPAGTESLALVVDDP
                     DAVGGLYIHWIVTGIPRTTTEIIDGALPDGAHVGLNSGGQAAYLGPCPPAGTGVHHYR
                     FQLYAIGKPLEVPPSTPAQDATRAIAEAAIVDVRIVGLFSG"
     gene            3187..3810
                     /gene="upp"
                     /locus_tag="VF874_19520"
     CDS             3187..3810
                     /gene="upp"
                     /locus_tag="VF874_19520"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX9178165.1"
                     /translation="MDVRVVDHPLARTRLTTLRDERTDNAAFRAALRDLTLMLVYEAT
                     RDAPTEAVAVRTPLADTTGCRLANPPLLVPVLRAGLGMVDQAHALIPEARVGFVGVAR
                     DEETHSPTPYLESLPDDLSGQPVLVLDPMLATGGSMAHIVGLLKARNADDITAVCVVV
                     APEGIAALEPIAPDMRVFTATIDERLNEIAYIVPGLGDAGDRQFGPR"
     gene            complement(3792..5366)
                     /locus_tag="VF874_19525"
     CDS             complement(3792..5366)
                     /locus_tag="VF874_19525"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEX9178166.1"
                     /translation="MTPEEWIAHDPDPDAAAELSALDADELAARFSGSLTFGTAGLRG
                     PMRAGPTGMNVAVVLRATWALARVLRNRGLSGSEVFVGYDARHGSRTFAVATAEVLAA
                     GGFSVTLLPEPLPTPVLAFAVRHTGAAAGVQITASHNPATDNGYKVYLDGGLQIVSPT
                     DREIEAAIAAAPYADEIGRTPVTPGGDDIFERYLQRAAAVRRGSGTVRVALTPLHGVG
                     GAAAVEALRRAGFTDIHTVPSQFAPDPDFPTVTFPNPEEPGAVDALLDLAACVDADIA
                     VALDPDADRCAIGIPVDDGRRMLSGDETGWLLGDYILRHTEPGAVVASTMVSSRMLAA
                     IAAARGARHVETLTGFKWLARAAGGDLVYAYEEAIGHCVDPEAVRDKDGISAAVLCCD
                     LAAVTKAAGRGIPDLLDDLARRHGVHVTASITARTDEAEAVMARLRSDPPTRLAEFDV
                     TVTDVAGVLILTGSAGEAWVRLAVRPSGTEPKVKGYIEIRLPVAGDLTTARGTAEATG
                     EAVRRAAAALLQRGPN"
     gene            complement(5375..>5860)
                     /locus_tag="VF874_19530"
     CDS             complement(5375..>5860)
                     /locus_tag="VF874_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX9178167.1"
                     /translation="AAAGVRAVVLTNAAGALRDDYRVGQPVLISDHLNLTGRSPLVGP
                     QFVDMVDAYSTRLRALARDVDPSLQEGVYAGLLGPQYETPAEISMLRTLGADLVGMST
                     VHETIAARAAGLDVLGLSLVTNLAAGITGQPISHDEVLDAGRESAARMGALLAAVVTA
                     F"
BASE COUNT          873 a         2069 c         2039 g          879 t
ORIGIN      
        1 cgcgggtgat cagcggtcgc tggcgatcgg tatggccggg cgggtgatgg agctgttttt
       61 caatgcgcgc cgccgtgacg catcacggct cgccgccgaa cacgtagagc tgctcgagtc
      121 gatcggcgat ccgatgttga cgctggcatt gctccctact gtgatatccg ccaagcacga
      181 gaccgccgag atgtccgacg tcttgcgtct ggcccagcgt ggcatcgacc tcgcgggcgg
      241 cgacgccacc aaaggtggcg agctggcaac cagttctccg ctgacgatgg ctctcgccta
      301 ccgcggtttg gcccgctggg gttttgggat caccggttgg cgagagaatt tctcccaggc
      361 catcgcgctc gcccaaaccg gtgaggcggt gctgcgcggc ggtgcgatgt attacacgca
      421 cgtggtggcg gtcatgcacg gtgtggtgct gcctagtgcc gccgccgtgc ggaaagcgac
      481 cgaaacgctt gcgttggcag agcagaccgg tgaggacgtc gcgctgggat cggcccggtc
      541 caatctcggc tactttctcg tgtgtgtggg tggggattcg cgcgatcggg ggttggaatt
      601 gctcgcgctg gcaagggaac tcacgatgcg gcggcgatac gccatgagcg ggctgccgat
      661 catggacgtc atggccgctc gggtgcatac ggaactggga aacctcgaca ccgccatcga
      721 cctgtcgcga gccgcccttg atgaggtgct cgaaggcggt gggacgcttt gggcgccggt
      781 ggccgcgaat gtcctcgtgg aagcgttggt gacgcgcggt gccaacggag atctcgacca
      841 tgcgcagacg gcggtggacc gattggccgc tgtgccgatc gagccaggac tcgtcctgta
      901 cgacatctgg ctgctgcggt tgcgtgcgct tctggcgcat gcacgcggcg acgacgtcag
      961 ctaccgagga ttcgcggatc gctaccggaa gatggcgaca gacctcggct ttgaaggaca
     1021 catggcgatg gccgaggcga tgcgatgagg ccggtcgtca cgcggtaggc cggaagaaac
     1081 cctgaaagcc ttggttcagg ttcgtcgtgc ggatcggtga cagctgcacg ggatcgcccg
     1141 cctcgatcat catgccgttg ccgaccacca tcgcgacgtg gccgtcccac accgcgaggt
     1201 cgcccggcag cagcgcgttc ggcgacacgg ccgccccgac gtcctgctct tgagccaacc
     1261 ggggaaggtt gagccccgcc tcgtcatacg cccactgcgt cagcccgctg cagtccagtc
     1321 cgacgcctgg cgtcgtccca ccccagtcgt agggcacgcc gagctgagtg agcgcatgac
     1381 ggacggcgtc cgcggccacc cggttcggcg ccttgacggt gctgccgtcg ggcagccgca
     1441 ccgcgacgcc gctgcctggc cagtcggaat ccagcatcgg cgcctcggcg aaacccgcac
     1501 ccggcaggac gccggaggtc aggcccatca gggtcgacgg gacgcccgcg acggccggcg
     1561 acatcgacga ctgggggtac cacccgctcg cgggggagag gcccgccggg gacgtgacgg
     1621 gcgcggcagg catcaccgca gcacccgact cggtgactgc cgcggcgtgc ccgtcgagct
     1681 cggcttccag ctccgcgacg acggctgcgg cgtcggtcag tgcccgccgt gtggcgtcgg
     1741 ccagtgagcc ggcgcttgcc ggcagatcgc gttccagccg gtatgccgtg gtctcgaagt
     1801 cgtcgacgat ccgctgcagc tgcgccttcg cgcgcgcgac cgcctccgat gcggcgccca
     1861 cgcggctcgc catgcccgac gcgtgcgtcg cgaggtcggc ggcgctgctg agggccgtcg
     1921 agatcgtgcg cgtcgccgcg tcggcggcca gacccgacca catctcgccc acctggcccg
     1981 tggctgcggc tccggaggcc gccacgtccg tcagtactcg gctcgcgtcg agcagcatcc
     2041 tggcggggtc gtcgccggtg aacactccgg agccgacccg tgcctgcagc tcgtgcagcg
     2101 gcgccgtgag ggcggctacc aaagcgctgg ccactggcta aaccgttagg ttggcgactc
     2161 gcgcctgcgc ccgcagttcg gcctcgtcgt actgcccggc tgccgctgcc gtcgcgctgc
     2221 cgtgcgacaa aacctcgtcg ccgagcttgt tcacgctggt catcaaagcc tcggcggcgt
     2281 cgcctaacgc gtgggcgaag cgggcgccca ccggcccgag cgcgtcggtg acggaggggc
     2341 cgagatcgcg ggacaaggcg gccgccgccg cgcgaatatc ggtgcccagg tcgatggtgc
     2401 tcccgctgaa gccgcggatc gagttggtat cggcgtgcat acgacgttgg acgctgcggt
     2461 gcggccgcgc ggttccgtcg tcggcacttc cgaagtgccc cgggaagcgc caaaatatgg
     2521 gccatgaaat gggccgccgc cgctctggcg ctgctgctct gcgcgctgac cgcgtgtggc
     2581 ggcgccgaca agagcgcgaa ctacaccttg gactccacga cgccggggcc cgagacggcc
     2641 acgacgacga ccatgacctc gccgcccccg ccggtgtcgg cgccgttcgc gctgagcagt
     2701 cccgcgttcg ccgacaacac gccgatcccc gcggagtact cctgcaacgg ccgcaacgtt
     2761 cctccgccgc ttcgctggga gaacgttccg gcgggcaccg agtcgctggc tctggtcgtc
     2821 gacgaccccg acgccgtcgg cggcctctac atccactgga tcgtcaccgg catcccgcgc
     2881 accaccaccg agatcatcga cggcgcgctg cccgacggcg ctcacgtggg gctgaactcc
     2941 ggcggccagg cggcctatct aggcccgtgc ccgccggccg gcacgggcgt gcaccactac
     3001 cgattccagc tgtacgccat cggcaagccg ttggaggtac cgccctcgac accggcgcag
     3061 gacgccaccc gagcgatcgc tgaggcggcc atcgtcgacg tccgcatcgt cgggctgttc
     3121 agcggctgag gctggggtcc ccggcgtcgt caccggttac ccgcggccaa gctagggtca
     3181 accgccatgg acgtgcgcgt cgtcgaccac ccactggcca ggacccggct caccacattg
     3241 cgcgatgagc gcaccgacaa cgccgcattc cgggccgcgt tacgcgacct gacgctgatg
     3301 cttgtctacg aggccacccg cgacgcgccg accgaggccg tcgcggtgcg cacaccgctg
     3361 gccgacacca ccggctgccg gctggccaat ccaccgctgc tggtgccggt gctgcgggcc
     3421 gggctcggga tggtcgacca ggcgcacgcg ctgatccccg aggcgcgcgt cggtttcgtc
     3481 ggggtggcac gcgacgagga aactcattcg cctacaccgt atttggagtc gctgcccgac
     3541 gacctgtccg gccagccggt gttggtgctc gacccgatgc tggcgacggg cggctcgatg
     3601 gcgcatatcg tcgggctgct gaaggcgcgc aacgccgacg acatcaccgc ggtgtgcgtg
     3661 gtggtggcac ccgaaggaat cgcggcgttg gagcccatcg cgcccgatat gcgggtgttc
     3721 accgcgacca tcgacgagcg gctcaacgag atcgcttaca tcgtcccggg attgggcgat
     3781 gcaggcgacc gtcagttcgg cccgcgctga agcagcgcgg ccgccgcccg ccgcaccgcc
     3841 tcgcctgtgg cttccgcggt accgcgggcg gtggttaggt cgccggcgac gggaagccta
     3901 atctcgatgt accccttaac tttcggctcc gtgcccgacg gacgcaccgc tagccgtacc
     3961 cacgcctcgc cggcgctgcc ggtcagaatc agaacgcccg cgacatcggt gaccgtgaca
     4021 tcgaactccg ccagccgcgt cggtgggtcg ctgcgcagtc gggccatcac cgcttctgcc
     4081 tcgtcggtgc gtgcggtgat cgacgccgtg acgtgtacgc catggcggcg ggccaggtcg
     4141 tcgagaaggt ccgggatgcc gcggccggcg gccttcgtca cggccgccag atcgcagcac
     4201 agcaccgcgg cgctgatgcc gtccttgtcg cgcaccgcct ccgggtcgac gcagtggccg
     4261 atcgcctcct cgtacgcgta gacgaggtcg cctcctgccg cgcgggccag ccacttgaac
     4321 ccggtcaacg tctcgacgtg ccgggcgccg cgcgcggccg caatggccgc gagcatccgc
     4381 gacgacacca tggtgctcgc gacgacggcc ccgggctctg tgtgccgcaa gatgtaatca
     4441 cccagcagcc aaccggtttc gtcgccggac agcatgcgcc ggccgtcgtc gacggggata
     4501 ccgatcgcgc agcggtcggc gtcggggtcg agcgccaccg cgatgtcggc atcgacgcat
     4561 gctgcaagat caagcagcgc gtccaccgcg cccggctcct cgggattcgg gaacgtgacg
     4621 gtggggaagt cggggtcggg cgcgaattga cttggcaccg tgtgtatatc ggtgaacccg
     4681 gcccgccgca gcgcctcgac cgcggcggcg ccgccgacgc cgtgcagcgg ggtcaacgcc
     4741 acccgcaccg tccccgaacc tcggcgcacc gcggcggcgc gctgcaggta gcgctcgaaa
     4801 atgtcgtcac caccgggtgt cactggcgtg cggccgattt cgtcggcata gggcgccgca
     4861 gcgatcgcgg cttcgatctc acggtcggtg ggcgagacga tctgcaaacc gccgtcgagg
     4921 tacaccttgt agccgttgtc cgtcgccggg ttgtgtgacg cggtgatctg cacgccggcc
     4981 gccgcacccg tgtgccggac cgcgaacgcc aacaccggag tgggcagcgg ctccggcaac
     5041 agggtgacgg aaaagccgcc ggcggcaagt acttcggcgg tcgcgacggc gaatgtgcgg
     5101 gatccgtgcc gggcgtcgta gccgacgaac acctccgacc cgctcaagcc ccggtttctc
     5161 agcacccggg ccaacgccca agtggcgcgc agcaccacgg cgacgttcat gcccgtcggg
     5221 ccggcccgca tcggtccgcg cagcccggcc gtgccgaacg tcagcgaccc gctgaaccgc
     5281 gcggcgagct cgtcggcgtc aagcgccgac aactcggccg cggcatcggg atccggatcg
     5341 tgggcgatcc actcctcggg cgtcaccggt ccagtcagaa cgcggtgacg acagcggcca
     5401 gcagagcacc catccgcgcc gccgattcac ggcctgcgtc gagaacctcg tcgtggctga
     5461 tcggttgacc ggtgatgccg gcggccaagt tggtcaccag agacaacccg agcacgtcga
     5521 ggcccgccgc ccgcgcggcg atggtctcgt gcacggtgga catgccgacc aggtccgcgc
     5581 cgagcgtgcg cagcatgctg atctcggccg gcgtttcgta ctgcggaccg agcaggccgg
     5641 catacacccc ctcctgcagg gaagggtcga cgtcacgcgc caacgcccgt agccgggtgg
     5701 agtacgcgtc gaccatgtcg acgaactgcg gcccgaccag cggggaacgg ccggtcagat
     5761 tcaagtggtc ggaaatcagc accggctgcc cgacgcggta gtcgtcccga agcgccccgg
     5821 ccgcgttggt cagcaccacc gcgcgcacgc cggcagccgc
//
LOCUS       DASWBF010000525        22852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1313269,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000525 DASWBF010000000
VERSION     DASWBF010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313269"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VF874_19535"
     CDS             <1..779
                     /locus_tag="VF874_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178168.1"
                     /translation="GEAWRGQGADAASAALRRYVSGSLDGLIACRSLAVQLNELSRAA
                     GDLRACITAPATGDVAGQRLDEALSQVRRLYSAPAVAAGNAVADIPEPPEPFAVDGQV
                     PAGVQPADMPTGNPAAALPSTDAPLSFTPPPPPPTADSVPRPSLPGSEAQPVWRAPTH
                     STAFDPPVGDALPARLDAPATTPTPPPSGMPATTPGQSPSVAARPSSSSPFSPFLGYP
                     GLLGRDGGAEHRAPRYLISAGNTNELIGELPLVAPPVIGE"
     gene            complement(787..1131)
                     /locus_tag="VF874_19540"
     CDS             complement(787..1131)
                     /locus_tag="VF874_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX9178169.1"
                     /translation="MADMGAPRTGRERIKTLAQAALNADLTVEQVDTVLNSLMNSLSA
                     LDNSINGLDGSLETFNGSLNYLNDTLNRLEGIVERVERIVAVGEAAVAPLAATETTVR
                     QVVKAVRDRTHL"
     gene            complement(1140..1889)
                     /gene="pgl"
                     /locus_tag="VF874_19545"
     CDS             complement(1140..1889)
                     /gene="pgl"
                     /locus_tag="VF874_19545"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX9178170.1"
                     /translation="MSTSVHTYPDSAALVAAVGDRLAEEIASVLEFRGKASIVLTGGG
                     TGIGVLQHLSERGGHIDWSRVHLYWGDDRFVAEDDDERNFRQARLALLDKVDVPAENV
                     HPMAASDGAFGDDISAAAEGYERELAAQAEPGQPAPDFDVHLLGMGPEGHVNSLFPHT
                     DAVRERERLVLAVEESPKPPPRRITLTLPAIQRSREVWLIVSGSGKADAVAAAVNGAD
                     PDEVPAAGAVGRDATVWLLDDAAAAKLDDRS"
     gene            complement(1886..2797)
                     /gene="opcA"
                     /locus_tag="VF874_19550"
     CDS             complement(1886..2797)
                     /gene="opcA"
                     /locus_tag="VF874_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX9178171.1"
                     /translation="MIVDIPDTTTNDINKRITTLREEAGAITLGRVLTLVAAPDCEEI
                     VEGVIDAAASASREHPCRIIVVVTGDRSAANPRLDGQLRVGGDAGAGEVVVLRISGEL
                     ADHASSVVLPFLLPDTPVVAWWPGAAPAKPAEDPLGQLAIRRITDATHTAEPLAAIKS
                     RLGGYTPGDTDLAWSRITYWRALLTSAIDQPPHEPIKSALVSGLKEEPALDVLAGWLA
                     SRIDGPVQRAVGDLKVELNRQTETVTLSRPQTGHTATLSRTARPDALVPLARRETREC
                     LAEDLRRLDADEIYAEALRGIDKVQYV"
     gene            complement(2858..4408)
                     /gene="zwf"
                     /locus_tag="VF874_19555"
     CDS             complement(2858..4408)
                     /gene="zwf"
                     /locus_tag="VF874_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX9178172.1"
                     /translation="METKSAAPEAVWRNSLRDKRDKRMPHIAGPCGVVIFGVTGDLAR
                     KKLMPAIYDLANRGLLPPTFALVGFARRDWADEDFGQVVYEAVKHHSRTPFRQEVWDR
                     LCEGVRFVQGAFDDDAAFGRLAETLEKLDAERGTGGNHAFYLSIPPKAFPVVCEQLQK
                     SGLARQQEGKWSRVVIEKPFGHDLQSARELNAVVNSVFPEETVFRIDHYLGKETVQNI
                     LALRFANELIEPVWNAHYVDSVQITMAEDIGLGGRAGYYDGIGAARDVIQNHLLQLLA
                     LTAMEEPVSFHPHELQAEKIKVLSAVKLAEPLSETTSRGQYTAGWQGGEKVVGLLEEE
                     GFSKDSTTETFAAITLEVDCRRWAGVPFFLRTGKRLGRRVTEIALLFKRAPHLPFDAT
                     MTEELGQNALVIRVQPDEGITMRFGSKVPGTAMEVRDVNMDFSYGSAFAEDSPEAYER
                     LILDVLLGEPSLFPVNAEVELAWEILDPVLDYWASHGRPEPYPAGTWGPPSSFEMLRR
                     TGREWRRP"
     gene            complement(4414..5535)
                     /gene="tal"
                     /locus_tag="VF874_19560"
     CDS             complement(4414..5535)
                     /gene="tal"
                     /locus_tag="VF874_19560"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX9178173.1"
                     /translation="MMAQNENLAALSAAGVSVWLDDLSRERLRSGNLQELIDTKSIVG
                     VTTNPTIFQTALSKGHSYDAQVAELAERGADVDAAIRTVTTDDVREACDVLRPQWEAS
                     DGVDGRVSIEVDPRLAHDTDKTILQAIELWKIVDRPNLLIKIPATKAGLPAITSTLAE
                     GISVNVTLIFSVERHRAVMDAYLAGLEAAKQAGHDVSKIHSVASFFVSRVDTEIDKRL
                     EKIGTDEALALRGQAGVANARLAYAAYEEVFVGGERYDALKADGARVQRPLWASTGVK
                     NPEYSDTLYVTELVAPNTVNTMPEKTIDAVAEHGVITGDTVTGRADEAQEVFDKLDAV
                     GIDLPDVFLVLEDEGVEKFEKSWHELLEATQEQLDAAKK"
     gene            complement(5545..7635)
                     /gene="tkt"
                     /locus_tag="VF874_19565"
     CDS             complement(5545..7635)
                     /gene="tkt"
                     /locus_tag="VF874_19565"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9178174.1"
                     /translation="MTTLEEISALTRPNHPDDWTDLDSAAVDTIRVLAADAVQKVGNG
                     HPGTAMSLAPLAYTLFQRAMCHDPSDTHWLGRDRFVLSAGHSSLTLYLQLYLGGFGLE
                     LSDIESLRTWGSKTPGHPEFRHTAGVEITTGPLGQGLASAVGMAMASRYERGLFDPDA
                     APGTSPFDHFIYVIASDGDIEEGVTSEASSLAGTQQLGNLIVFYDSNQISIEDDTKIA
                     LNEDTPARYEAYGWHVQIVEGGENVVGIDEAIAAAKAVTDKPSFIAIRTVIGYPAPKL
                     MNTGKAHGAALGDEEVAAVKEILGFDPDKKFEVRDEVLAHTRKLVDRGREAHQKWQAD
                     FDAWAEREPDRKALLDRLTAQELPDGWDEELPYWEPGSKPLATRAASGEVLTALGPKL
                     PELWGGSADLAGSNNTSIKDADSFGPPSISTKEYTAHWYGRTLHFGVREHAMGAILSG
                     IVLHGPTRAYGGTFLQFSDYMRPAVRLASLMNIDTVYVWTHDSIGLGEDGPTHQPIEH
                     LAALRAIPNLSVVRPGDPNETAYAWHTILCRGAGSGPVGLILTRQGIPVLEGTSADGV
                     AKGGYVLGGRPDEEPDVILIGTGSELQLAVEAQKILADKGIQASVVSMPCVEWFESQP
                     VEYRDSVLPPAVSARVAVEAAVAQSWYKLVGDTGEIVSIEHYGESADAKTLFREFGFT
                     PEAVVAAAERTLDN"
     gene            7871..8797
                     /locus_tag="VF874_19570"
     CDS             7871..8797
                     /locus_tag="VF874_19570"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9178175.1"
                     /translation="MSIRERARVSWPNRTRSTLLGYFALTKPRVIELLLVTAIPAMLL
                     ADRGTVAPLLILNTLIGGMLAAGGANTLNCVADADIDKVMKRTACRPLPRAAVPTRHA
                     LVFGLALSVASFFWLWWTTNLLSGLLAVATIAFYVFVYTLLLKRRTSQNVVWGGAAGC
                     MPVMIGWSAVTGTINWPALAMFAIIFFWTPPHTWALAMRYKEDYQAAGVPMLPAVASE
                     RHVTKQILVYTWLTVAATLVLALAAGWLYASVAVLAGVWFVTMAHQLYAAVCRGEPVK
                     PLRLFLQSNNYLAVVFCALAVDSALALPTLLH"
     gene            complement(8856..9830)
                     /locus_tag="VF874_19575"
     CDS             complement(8856..9830)
                     /locus_tag="VF874_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX9178176.1"
                     /translation="MHAIEVAEHGGPEVLRYVEKPQPSPGPGQVLIKAEAIGVNYIDT
                     YFRSGAYPREVPFVLGQEVCGTIEAVGGDVAALNVGDRVVTVATNGAYAEYSVAPADF
                     VAYVPDGVAPDIAASALLKGMTAHYLIKSVYPVEQGDTVLVHAGAGGVGLLLTQWATS
                     LATKVITTVSTPEKAELSRRAGAVEVLDYPFDPETFGARIRELTDGKGVAAVYDGVGK
                     TTFDASMASLAVRGTLALFGAASGPVPPVDPQRLNAAGSVYLTRPSLVHFTRTPDEFT
                     WRAGEVLDAIAAGSITVTVSGRYPLAEAARAHEDLQGRKTTGSIVLVP"
     gene            9849..10790
                     /locus_tag="VF874_19580"
     CDS             9849..10790
                     /locus_tag="VF874_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178177.1"
                     /translation="MKLARPNVFHPRIVLAGCRQLVDGDGDDAGLIPALSRRGLHARW
                     RSWDDPDTLRADVVILRATWDYAERLDEFVAWTTAVRHLLNAPAVVAWNADKRYLLDV
                     ERRGVPIVPTEVFGPSDRVRLPAGEVVVKPSIGVGSVDAQRFTDRAAAQAHAAALQEQ
                     GRSVLVQPYDDRVERAGETALVFLGGKPSHAFTKAAMLPPQGQRIELDSTGTYAIETL
                     APADPDFEIWDVGFAALDAAAQQLDIAAGDFLYARVDVIGGIDDPRLLELELIEPSLG
                     WTQLDERTRDLQQREFARRLSSALERLGLGPLSHRRP"
     gene            complement(10740..11651)
                     /locus_tag="VF874_19585"
     CDS             complement(10740..11651)
                     /locus_tag="VF874_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HEX9178178.1"
                     /translation="MPSLRTQRLIAAAVILTQGGIAVTGAIVRVTASGLGCPTWPQCF
                     PGSFTPVAVSEVPRIHQAVEFGNRMVTFLVVLTAALAVLAVTRARRRAEVQVYAWLMP
                     ASTVVQAVIGGITVLTGLLWWTVALHLLASMTMVWLAVLLFVKVGEPDDGVPRLVAPK
                     PLRWLVVLSAVALAAVLVTGTLVTASGPHAGDTSPTRTVPRLAIEITTLVHAHSSLLI
                     GYLALLVGLGFGLLAVSAPRPVLLRLAVLIALVVAQGLVGTVQYFAGVPAVLVAVHVA
                     GAAAVTAATAALWASMRERTKPEPLQR"
     gene            complement(11945..12721)
                     /locus_tag="VF874_19590"
     CDS             complement(11945..12721)
                     /locus_tag="VF874_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9178179.1"
                     /translation="MTETRFAPETFTPDPRPNTVQRMLAAQYGLELKLLLRNGEQLLL
                     TLFIPVTLLVGLTLLPLGSFGDNRAATFVPAIMALAVISTAFTGQAIAVAFDRRYGAL
                     KRLGATPLPVWGIIAGKSLAVVTVVFLQAIILGGIGFGLGWRPQLTGLMLGAVVIALG
                     TAAFAALGLLLGGTLKAEVVLALANLMWFVFAGFGALTLGGGLVPRGVEWVARLTPSG
                     ALTEALSQAMTVSVDWFGLCVLAVWGTLAGLAALRLFRFT"
     gene            complement(12718..13647)
                     /locus_tag="VF874_19595"
     CDS             complement(12718..13647)
                     /locus_tag="VF874_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9178180.1"
                     /translation="MTSTAPIPVRLRGVCKRYGSTTAVSDLDLDVQRAEVFALLGPNG
                     AGKTTTVEMCEGFVRPDAGTIEVLGMNPVTDNARVRERIGVMLQGGGGYPAARAGEML
                     NLVAAYAANPLDPKWLMDTLGLTDVARTTYRRLSGGQQQRLALACALVGRPELVFLDE
                     PTAGLDAHARLVVWELIDALRRDGVTVVLTTHQLTEAEQLADRLVIIDRGVAVAAGTP
                     AEIMRNGAENQLRFTAPRMLDLSLLSAALPEGYKATEVSSGQYLVEGPINPQVLATVT
                     AWCARLDVLATDMRVEQRSLEDVFLELTGREVR"
     gene            complement(13644..15365)
                     /gene="mptB"
                     /locus_tag="VF874_19600"
     CDS             complement(13644..15365)
                     /gene="mptB"
                     /locus_tag="VF874_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HEX9178181.1"
                     /translation="MAARRHGSLSLSIARWHGDERTVGSPLNDAEVVALRRTRLFGAT
                     GTVLMAIGSLGAGARPVVQDPTFGVRLLNLPSRMQTVSLTMTTTGAVMMALAWLMLGR
                     FALGERRMSRSQLDRTLMLWILPLLIAPPMYSKDVYSYLAQSEISRLGLDPYRVGPAP
                     ALGLDHVFTLSVPSLWRETPAPYGPLFLWIGRGISSLTGENIVAAVLCHRLVVLIGVG
                     LIVWATPRLARRCGVAEVSALWLGATNPLLIMHLVAGIHNEALMLGLMLAGTEFALRG
                     VDATRALLPRPLRWPRHRADWEPWYPPAMLVAGVVLITLSSQVKLPSLLALGFVAMAL
                     ARRWGASIRGVIAAGGLLGLMSLAVTAAIGLASGLGFGWVNTLGTANVVRSWMSPPTL
                     LALGTGQVGILLGLGDHTTAVLGLTRAIGVLIIAILVSWLLIAVLRGRLHPIGGLGVA
                     LGATVLLFPVVQPWYLLWAIIPLAAWATRPRFRIATIIVTLVVGIFGPTANGDRFALF
                     QIIDATVASAVIVLILIALTYSMLPWRSVPHPVTGSEEAAEPDVPKPISPAPTTSPVP
                     GAYAESP"
     gene            15463..16167
                     /locus_tag="VF874_19605"
     CDS             15463..16167
                     /locus_tag="VF874_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9178182.1"
                     /translation="MKIRPETDRHTRGEIVQLLLEAGPITAGAIGERLGISAAGVRRH
                     LDALLDAGDAQAGPAAAWQQHGRGRPAKRYRLTPAGRAKLGHTYDDLAAAAMRQLREI
                     GGDEAVRTFARRRIDTILAGVGDGPDDVEATAERVAAALTKAGYATTTTRVHGQVDGI
                     QICQHHCPVSDVAEEFPELCQTEREAFAEILGTHVQRLATIVNGDCACTTHVPLTDPS
                     AHSPRGGKTKLEGASI"
     gene            16164..18647
                     /gene="sufB"
                     /locus_tag="VF874_19610"
     CDS             16164..18647
                     /gene="sufB"
                     /locus_tag="VF874_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX9178183.1"
                     /translation="MTATPDTSTHKIGEPLSQDEAIASLSRYGYGWVDSDDAGASAKR
                     GLSEDVVRDISAKKNEPEWMLQQRLKALRIFRRKPMPTWGSNLEGIDFDNIKYFVRST
                     EKQAASWDDLPDDIKNTYDRLGIPEAEKQRLVAGVAAQYESEVVYHQIREDLEAQGVI
                     FLDTDTGLREHPDIFKQYFGTVIPAGDNKFSALNTSVWSGGSFIYVPPGVRVDIPLQA
                     YFRINTENMGQFERTLIIADEGAYVHYVEGCLPAGELVTMADGNLRPIDSIRVGDSVM
                     SHDGKPHRVNAIQVRDLNGELFTFTPMSPANAFSVTAEHPLLVVPREEVRVARKARNG
                     WKAEVDTAKLRCAEPRWIAAKDVAEGDFLVYPKPKPIPHKTVLPLEFARLAGYYLAEG
                     HATLTNGCESLIFSFHSDEFDYVEDVRQACKSLYETAGSVLVEEHKHSARVTVYTKAG
                     YAAMRDNVGVGSANKRLSDLLMRQDETFLRELVDAYVNGDGNVIRRGGALWKRVHTTS
                     RVWAFQLQSILARLGHYATVEHRKDLYQVQWTEGGSGPKQARDCGDYFAVPIKKRAVR
                     EAHEPVFNLDVEHPDSYLAYGFAVHNCTAPIYKSDSLHSAVVEIVVKPHARVRYTTIQ
                     NWSNNVYNLVTKRARAEAGATMEWIDGNIGSKVTMKYPAVYMVGEHAKGEVLSVAMAG
                     EGQHQDTGAKMLHLAPNTSSNIVSKSVARGGGRASYRGLVQVNKGAHGSRSSVKCDAL
                     LVDTISRSDTYPYVDIREDDVTMGHEATVSKVSEDQLFYLMSRGLTEDEAMAMVVRGF
                     VEPIAKELPMEYALELNRLIELQMEGAVG"
     gene            18710..19855
                     /gene="sufD"
                     /locus_tag="VF874_19615"
     CDS             18710..19855
                     /gene="sufD"
                     /locus_tag="VF874_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX9178184.1"
                     /translation="MAGANKGEIFTSFDVNAFEVPGGRDEIWRFTPLRRLSGLHDGSA
                     PATGSAHIEVAERPGVTVETVRRGDERLGQGGAPADRVAAQAFSSFNKATIVTVDKGA
                     TPGEPIEIKLTGPGEGKTAYGHLQVRVGELAEAVVVIDQQGSGTYADNVEFVLGDASR
                     LTVVSVADWADDAVHVTSHHAALGKDAVLRHIAVTLGGDVVRLTGRVKFCAPGGDAEL
                     LGLYFADDGQHFESRLLIDHAEPNCRSNVLYKGALQGDPESHKPDAHTVWVGDALIRA
                     NATGTDTFEVNRNLVLTDGARADSVPNLEIETGEIIGAGHASATGRFDDEQVFYLRSR
                     GIPEDQARRLIVRGFFNEIIAKITVPAIRERLTDAIEYELALTEGRL"
     gene            19852..20622
                     /gene="sufC"
                     /locus_tag="VF874_19620"
     CDS             19852..20622
                     /gene="sufC"
                     /locus_tag="VF874_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX9178185.1"
                     /translation="MTTLEIKDLHVSVTPSEGEEIPILKGVDLTVKSGEMHALMGPNG
                     SGKSTLSYAIAGHPKYKVTSGSITLDGEDVLAMSVDERARAGLFLAMQYPVEVPGVSM
                     SNFLRTAATAVRGEAPKLRHWIKEVKAAMDDLDIDPAFSERSVNEGFSGGEKKRHEIL
                     QLDLLKPKIAILDETDSGLDVDALRVVSEGVNRYAEETHGGLLLITHYTRILRYIEPQ
                     FVHVFIGGRIIESGGPELADELDANGYERFTEKAAAGA"
     gene            20625..21878
                     /locus_tag="VF874_19625"
     CDS             20625..21878
                     /locus_tag="VF874_19625"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX9178186.1"
                     /translation="MTTSARTLDIDAIRADFPILKRVMRGGKQLAYLDSGATSQRPVQ
                     VLDAERDFLTTSNGGVHRGAHQLMEEATDAYEQGRADIAAFVGADADELVFTKNATEA
                     LNLVAYVLGDNRFERAVGPGDVIVTTELEHHANLVPWQELAQRTGATLQWYSVTDDGR
                     IDLDSLQLDERVKVVTFSHHSNVTGALAPVSELVSRARAVGALTVLDACQSVPHQPVD
                     LHALDVDYAAFSGHKMLGPNGIGMLYGRRELLNAMPPFITGGSMIETVTMEATTYAPA
                     PQRFEAGTPMTSQVVGLAAAARYLQAIGMDTVEAHERELVAAALDGLSGIEGVRIIGP
                     TSLTDRGSPVAFVVDGVHAHDVGQVLDDDGVAVRVGHHCAWPLHRRFGISATARASFA
                     VYNTFDEVDRLVAGVRRAVDFFGGP"
     gene            21880..22374
                     /locus_tag="VF874_19630"
     CDS             21880..22374
                     /locus_tag="VF874_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX9178187.1"
                     /translation="MRLEQIYQEVILDHYKHPHHRGLREPYGAEVFHVNPTCGDEVKL
                     RVALSDDGESVADVSYDGQGCSISQASTSVLTDQVIGQSVGRALDIVGAFTEMVSSRG
                     TVEGDEEVIGDGIALAGVSKYPARVKCALLGWMAFKDALAQASTRIDVQEVDDERNGR
                     SIAG"
     gene            22343..22696
                     /locus_tag="VF874_19635"
     CDS             22343..22696
                     /locus_tag="VF874_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX9178188.1"
                     /translation="MSETAAQLPDEEFLADLEEAMRDVVDPELGINVVDLGLVYGLNV
                     ERGEDGTTALIDMTLTSAACPLTDVIEDQSRMALVGAGLVNEIKINWVWNPPWGPDKI
                     TEDGREQLRALGFTV"
BASE COUNT         3880 a         7620 c         7667 g         3685 t
ORIGIN      
        1 tgggggaggc gtggcgaggc cagggcgccg acgcggcgtc ggcggcgttg cggcgctacg
       61 tcagcggctc gctcgacggg ctgatcgcat gccgatcgct ggcagtgcaa ctcaacgagc
      121 tgtcccgggc cgccggcgat ctgcgcgcgt gtatcaccgc tcccgctacc ggtgacgtcg
      181 ccgggcaacg cctcgacgag gctctgtcgc aagtccgccg gctctacagc gcgcccgccg
      241 tggccgctgg caatgctgtc gccgacattc ccgagccgcc tgagccgttc gcggtcgacg
      301 gccaagtacc cgcgggcgtg cagcccgccg atatgccgac ggggaacccg gccgccgcgc
      361 tgccgtcgac cgatgcgccg ctgagcttta cgccgccgcc acctccgccg accgcagaca
      421 gtgttccgcg gcccagcctg ccggggtccg aagcgcagcc tgtatggcgc gcacccactc
      481 acagcaccgc gttcgacccg ccggtcggcg atgcgctgcc cgcacgcctt gacgcccccg
      541 ccacgacgcc gaccccgcca ccgtccggaa tgcccgcgac tacgcccggg cagtcaccga
      601 gcgtcgctgc ccgcccgagt tcgtcctcac cgttctcgcc atttctgggt tatccgggtc
      661 tactcggacg cgacggcggc gcagaacatc gggcgccgag gtacctgatc tcggcgggca
      721 acaccaacga attgatcggt gagttgccgc tggtggcgcc tccggtgatc ggcgagtgag
      781 cgccgactag aggtgcgtgc ggtcgcggac ggccttcacc acctgacgca ccgtggtttc
      841 ggtcgccgcc agcggagcga cggcggcctc gccgacggcg acaatgcgct cgacgcgttc
      901 gacgatcccc tcgaggcggt tgagtgtgtc gttgaggtag ttcaggctgc cgttgaaggt
      961 ctccagcgaa ccgtcgagcc cgttgatgga attgtcaagt gccgacaacg aattcatcaa
     1021 gctgttcagc acggtgtcga cctgctcgac ggtgaggtca gcgttgagcg ccgcctgagc
     1081 cagcgtcttg atccgctccc ggcctgtgcg cggggcaccc atgtctgcca tgccggtcat
     1141 catgacctgt cgtcgagctt cgccgcggcc gcatcgtcca gcagccacac ggtggcgtcc
     1201 cggccaacag caccggcggc gggcacttcg tcggggtctg cgccgttgac cgcggccgcc
     1261 acggcgtcgg ccttgcccga cccggagacg atcagccaca cctcacgtga acgctgaatc
     1321 gccggaagag tcaacgtgat tcgtcgaggc ggcggcttgg gcgattcctc gacagcaagc
     1381 accagccgct ccctttcgcg caccgcatcg gtgtgcggga acagcgagtt gacatgacct
     1441 tccggaccca tgcccagcag atggacgtcg aaatccggcg ccggctggcc gggttcggcc
     1501 tgagcggcca gttcccgctc gtagccctcg gccgcggcag agatgtcgtc tccgaacgcg
     1561 ccatcgctgg ctgccatcgg atgcacgttc tcggcgggga cgtcgacctt gtcgagcaga
     1621 gccaggcggg cctgcctgaa gttgcgctcg tcgtcgtcct cggcgacgaa acggtcatcg
     1681 ccccaataca gatgcaccct cgaccagtcg atgtgaccac cgcgctcgct caggtgttgc
     1741 agcacaccga tgccagtgcc gccgccagtc aacacaatcg aggccttgcc gcggaattcc
     1801 aacaccgacg cgatctcctc ggcgaggcgg tcgccgaccg cggccaccag cgcggcgctg
     1861 tccgggtagg tgtgcacgga agtgctcata cgtactgcac cttgtcgatc ccgcgcagcg
     1921 cttccgcgta gatctcgtcg gcgtccagcc ggcgcagatc ctccgcgagg cactcgcgag
     1981 tttccctgcg cgccaagggc acgagggcgt cgggtcgggc tgtgcggctc agcgtcgcag
     2041 tgtggcccgt ctgcggccgg ctcaacgtca ccgtctcggt ctgccggttg agttcgactt
     2101 tcaggtcgcc gaccgcgcgc tgcaccggac cgtcgatccg gctggccagc cacccggcca
     2161 agacgtccaa cgccggctcc tccttgagcc cggacaccaa tgcggacttg atcggttcgt
     2221 gaggcggctg atcgatcgcc gaggtgagca gtgcgcgcca gtaggtgatt cgactccacg
     2281 ccagatcggt gtctcccggc gtgtagccac ccagtcggct cttgattgct gcgagtggtt
     2341 cggcggtgtg cgtcgcgtcg gtgatacgac ggatcgccaa ctggcccaac ggatcttcgg
     2401 ccggcttggc cggtgccgca ccgggccacc atgccaccac gggcgtgtcg ggcagcagga
     2461 acggcagcac cacgctgctg gcgtggtcgg ccagttcacc ggagatccgc agaaccacca
     2521 cctcgcccgc gccggcgtca ccgccgaccc gcagttgccc gtcgagccgc gggttcgcgg
     2581 cgctgcggtc cccggtgacg accacgatga tccggcacgg atgctcgcgg ctggcagacg
     2641 ccgcggcgtc gatcacgcct tcgacgatct cttcgcaatc gggtgcggcg acgagcgtca
     2701 gcacgcggcc gagcgtgatg gcgccggcct cttcgcgcag agtggtgatg cgcttgttga
     2761 tgtcgttggt cgtggtgtca ggaatgtcga cgatcatgat tgcctcttct tcgcgcaagc
     2821 gctcatcagt gccctctctt cttcgcgcaa gcgctcatca gggacgccgc cattcccggc
     2881 ccgtacgccg caacatctcg aacgacgatg gtgggcccca tgtgccggcc ggatacggtt
     2941 cgggccggcc atgtgacgcc cagtagtcga gcacgggatc gagtatctcc caggccaatt
     3001 cgacctcggc gttgacggga aacagggacg gttccccgag cagcacgtcg aggatcagcc
     3061 gctcgtaggc ctccggtgag tcctcggcga acgccgagcc gtacgagaaa tccatgttga
     3121 cgtcgcgtac ttccatcgcg gtgccgggca ccttcgaccc gaaccgcatc gtgatgccct
     3181 cgtcgggctg aacgcggatc accagcgcgt tctggccgag ctcttcggtc atcgtcgcat
     3241 cgaacggaag gtgcggcgcc cgcttgaaca gcagcgcgat ctcggtcacc ctgcggccca
     3301 accgttttcc cgtcctcagg aagaatggca ccccagccca ccgccggcag tcgacctcca
     3361 acgtgatcgc cgcgaacgtc tcggtggtcg aatccttgga gaagccctct tcctccagca
     3421 ggccgaccac cttctcgccg ccctgccatc ctgcggtgta ctgaccgcgc gacgtggttt
     3481 cgctcagcgg ctcggcgagc ttcaccgccg acagcacttt gatcttctcc gcctgcagct
     3541 cgtgtggatg gaagctgacc ggttcctcca tggcggtgag cgcgagcagc tgcaacaggt
     3601 ggttctgaat cacgtcgcgg gcagcgccga tgccatcgta atagcctgcg cgaccgccca
     3661 atccgatgtc ctcggccatc gtgatctgca cggagtcgac atagtgagcg ttccacaccg
     3721 gctcgatcag ctcgttcgcg aagcgcagag ccagaatgtt ctggacggtc tccttgccga
     3781 gatagtggtc gatgcggaac accgtctcct cgggaaacac gctgttgacc accgcgttga
     3841 gctcgcgcgc gctttgcagg tcatgaccga acggcttctc gatcaccacc cggctccact
     3901 tgccctcctg ctggcgggcg agccctgact tctgcagctg ctcgcacacc accgggaacg
     3961 ccttgggcgg aatcgacagg tagaacgcgt gattgccgcc ggtcccgcgt tcggcgtcga
     4021 gtttctccaa cgtctcggcc agccgcccga aggccgcgtc gtcgtcgaag gccccctgta
     4081 caaaccggac gccttcgcac agccggtccc acacctcctg ccgaaacggc gtgcgggagt
     4141 ggtgcttgac cgcctcgtag accacctggc cgaaatcctc gtcggcccag tcgcgtcggg
     4201 cgaagccgac cagcgcgaag gtcgggggca gcagtccccg gttcgcgagg tcgtagatcg
     4261 ccggcatgag tttcttgcgg gccaagtcgc cggtgactcc gaagatcact accccgcacg
     4321 gaccggcgat gtggggcatg cgtttgtcac gcttgtcgcg caacgagtta cgccacaccg
     4381 cttctggcgc tgcggatttc gtctccatcg ctgtcatttc ttcgcggcgt cgagctgttc
     4441 ttgcgtggcc tccagcagtt cgtgccatga cttctcgaac ttttcgacgc cttcgtcctc
     4501 cagtacgagg aagacgtcgg gcagatcgat gccgaccgcg tccagcttgt cgaatacctc
     4561 ctgcgcctcg tcggctcggc cggtgacggt gtcgccggtg atcacgccat gctcggcgac
     4621 cgcgtcaatc gtcttctcgg gcatcgtgtt gaccgtgttc ggcgcgacaa gctcggtgac
     4681 gtacagggtg tcggagtact cggggttctt cacgcccgtc gacgcccaca gtggccgctg
     4741 cacccgagcg ccgtcagcct tgagcgcgtc gtaccgctcg ccgccgacga acacctcttc
     4801 atacgcggcg taggccagcc gggcgtttgc gacaccggcc tgcccgcgca gcgccagcgc
     4861 ttcgtcggtg ccgatcttct ccagccgctt gtcgatctcg gtgtccaccc gggagacgaa
     4921 gaacgaagcg acagaatgga tcttcgacac gtcatggccg gcctgcttgg ccgcctccag
     4981 cccggccagg tacgcgtcca tcaccgcgcg gtgccgctcg acggagaaga tcaacgtgac
     5041 gttcaccgag atgccttcgg ccagcgtcga tgtgatggcc ggcaggcccg ccttggtggc
     5101 ggggatcttg atcagcaggt tcggccggtc gacgatcttc cacagctcga tcgcctgcag
     5161 aatcgtcttg tcggtgtcgt gggcgagccg ggggtcgacc tcgatggaaa cccggccgtc
     5221 cacgccgtcg gacgcctccc actgcggccg cagtacgtcg catgcctcgc gcacgtcgtc
     5281 agtggtgacc gtgcggatcg cggcgtcgac gtcggccccg cgctcggcca gttcggcgac
     5341 ctgggcgtcg tacgaatggc ctttcgacag cgcggtctga aagatcgtcg ggttggtggt
     5401 aactccgaca atggatttgg tgtcgatgag ctcctgcagg tttcccgatc gaagccgctc
     5461 gcgggacagg tcgtcgagcc agacggatac gccggccgca gacagtgccg cgaggttttc
     5521 gttttgcgcc atgatcagcc ctttctagtt gtccagtgtc cgttccgcgg cagcaacgac
     5581 ggcctcgggg gtgaacccga actcgcggaa caacgtcttt gcgtcggcgg actcgccgta
     5641 gtgctcgatc gacacgatct cgccggtgtc accgaccagc ttgtaccagc tctgcgctac
     5701 cgcggcctcg accgcgacgc gcgccgacac cgcaggaggc agcacggaat cgcggtactc
     5761 gaccggctgg gattcgaacc actccacgca cggcatcgac acgacgctgg cctgaatccc
     5821 cttgtccgcc agtatcttct gggcctccac cgcgagttga agctccgaac cggtgccaat
     5881 caggatcacg tcgggttcct cgtcgggccg cccgcccagc acgtaaccgc ccttggcgac
     5941 gccgtcggcg ctggtgcctt ccagcaccgg aatgccttgg cgcgtcagga tcaacccgac
     6001 cggaccgctg cccgcgccgc ggcacaagat cgtgtgccat gcgtacgcgg tctcgttggg
     6061 gtcgccgggc cgcaccacgg aaaggttcgg gatcgcccgc aacgcggcaa ggtgttcgat
     6121 cggctggtgg gtcggtccgt cttcgcccag cccgatcgag tcgtgcgtcc acacgtagac
     6181 ggtgtcgatg ttcatcagcg acgcgagccg caccgccggc cgcatatagt cggagaactg
     6241 tagaaacgtt ccgccgtacg cccgcgtcgg cccgtgcagc acgatgccgg acagaatcgc
     6301 acccatcgcg tgctcgcgga caccgaagtg cagtgtgcgg ccgtaccagt gggcggtgta
     6361 ctccttcgtc gaaatcgatg gtggcccaaa cgaatccgca tccttgatac tggtgttgtt
     6421 gctgccggcc aggtccgccg acccgcccca cagctcgggc agcttgggtc ccagcgcggt
     6481 cagcacctca cctgaggccg cccgggtggc cagcggcttg ctgcccggct cccagtaggg
     6541 cagctcctcg tcccagccgt cgggcagctc ctgcgcggtc agccggtcca gcaacgcctt
     6601 gcggtccggc tcgcgttcgg cccacgcgtc gaagtcggct tgccacttct gatgagcttc
     6661 gcggccgcgg tccaccagct tgcgggtgtg cgccagcacc tcgtcgcgca cttcgaattt
     6721 cttgtccggg tcgaagccga ggatctcttt gaccgccgcg acctcttcgt cgcccagcgc
     6781 cgcgccgtgc gccttgccgg tgttcatcag tttcggtgcg ggataaccga tcacggtgcg
     6841 gattgcgatg aacgacggct tgtcggtgac ggctttggcc gcggcgatcg cctcgtcgat
     6901 accgaccacg ttctcgccgc cctcgacgat ctgcacgtgc cagccgtacg cctcgtagcg
     6961 cgccggggtg tcctcgttca gcgcgatctt ggtgtcgtcc tcgatcgaga tctggttgct
     7021 gtcgtagaac acgatgaggt tgccgagttg ctgggtcccg gccagagacg acgcttccga
     7081 cgtcacgccc tcttcgatgt cgccgtcgga ggcgatcacg tagatgaagt ggtcgaacgg
     7141 gctggtgccc ggcgcggcgt cgggatcgaa caggccgcgt tcgtagcgcg acgccatcgc
     7201 catcccgact gccgacgcga ggccctgacc cagcgggccg gtggtgatct cgacgccggc
     7261 ggtgtgccgg aactcggggt ggccgggcgt cttggagccc cacgtccgca gcgactcgat
     7321 gtcggagagt tccagcccga acccgccgag gtagagctgc agatacagcg tcaggctgct
     7381 gtgcccggcc gacagcacga accggtcgcg gccgagccag tgggtgtcgc tcggatcgtg
     7441 gcacatggct cgctgaaaca gcgtgtacgc gagcggagcg aggctcatgg ccgtgccggg
     7501 gtgaccgttg cccaccttct ggaccgcgtc cgccgccagc acccggatcg tgtcgaccgc
     7561 ggccgagtcg aggtcggtcc agtcgtccgg atggttgggg cgggtaaggg cggagatttc
     7621 ctcaagcgtt gtcacaaacg tgctcctcaa gtcggtagca ggccctagtc aaccctagtg
     7681 cgggtagccg ggatggtcga tcgctctggg taccggtgat taagcatcgc gcagcagcgg
     7741 actcggccgc gtcggaattg ctgtgaatcg accctcaagt attggttgag cggcctcccg
     7801 gtctaccatc gcgtgtagta gaagctgagc gctcgcgcga agagcgacca catcgccgag
     7861 gagtagctgc gtgagcattc gcgagcgggc ccgggtgtcg tggcccaacc gcactcggtc
     7921 cacgctgctc ggctacttcg ccctgaccaa gccgcgcgtc atcgagttgc tgctggtcac
     7981 cgcgatcccg gcgatgctgc tcgcggaccg tggcactgtg gcgccgctgc tgattctcaa
     8041 cacgctgatc ggcggcatgc tcgcggccgg gggcgcgaac acgttgaact gcgtcgccga
     8101 cgccgacatc gacaaggtca tgaaacggac cgcgtgccgt ccgctgccgc gggccgccgt
     8161 gccgacccgg cacgcactgg tgttcggcct ggcgctgtcg gtggcctcgt tcttctggct
     8221 gtggtggacg accaacctgt tgtcgggatt gcttgcggtc gccacgattg cgttctacgt
     8281 gttcgtctac acgctgctgc tgaagcggcg gacgtcgcag aacgtggtgt ggggcggggc
     8341 cgccggatgt atgccggtga tgatcggctg gtccgcggtg acggggacca tcaactggcc
     8401 ggccctggcg atgttcgcga tcatcttctt ctggacgccg ccgcacacct gggcgttggc
     8461 gatgcgctac aaggaggact accaggcggc gggcgtgccg atgctgccgg ccgtggccag
     8521 cgaacgccac gtgaccaaac agatcctggt gtacacctgg ctcaccgtcg ccgcgaccct
     8581 ggtgctggcg ttggccgccg ggtggctgta cgcgtcggtc gcggtgctgg ccggcgtctg
     8641 gttcgtgacg atggcgcacc agctgtatgc cgcagtgtgc cgcggcgagc cggtgaagcc
     8701 gctgcgactt ttcctgcagt ccaacaacta tctggccgtg gtcttctgcg cgctggcggt
     8761 cgactccgcg ctggcgctgc ccacgcttct gcactgactc tgcgctgagg gcgtgggcta
     8821 ctcgaacttt cgcgccctgg acgcagagtc aacggtcacg gcaccagcac gatcgagccc
     8881 gtcgtcttgc gaccttgcaa gtcctcgtgt gcgcgggcgg cctcggcgag cggatagcgc
     8941 ccgctgaccg tcacggtgat cgacccggcg gcgatcgcgt cgagtacttc gcctgcccgc
     9001 caagtgaact cgtcgggagt gcgggtgaaa tgcaccagcg acggccgggt caggtacacc
     9061 gacccggcgg cgttgagccg ctgcgggtcc accggcggca ccggtccgct ggccgcaccg
     9121 aacaacgcca gcgtcccgcg gaccgcgagg ctggccatgc tggcgtcgaa cgtcgtcttg
     9181 cccacgccgt cgtacaccgc cgcgaccccc ttgccgtcgg tgagttcgcg gattctcgcg
     9241 ccgaacgtct caggatcaaa ggggtagtcc agcacctcga ccgcgccggc ccgccgggac
     9301 agctccgcct tctccggtgt cgacaccgtg gtgatcacct tcgtcgccaa actggtcgcc
     9361 cactgcgtga gcagcagccc gacaccgccg gctccggcat gcaccagcac cgtgtcgccc
     9421 tgttccaccg ggtagacgga cttgatcagg tagtgcgcgg tcatgccctt cagaagcgcg
     9481 gaggcggcga tgtccggagc cacaccgtcg ggcacgtagg cgacgaaatc ggctggggcg
     9541 acgctgtatt cggcgtacgc gccgttggtc gcgacggtga ccacccggtc gccgacgttc
     9601 agcgccgcga cgtcgccgcc gacggcctcg atggtgccgc agacctcctg gccgaggacg
     9661 aacggcacct cgcgcgggta cgcgccggag cggaagtagg tatcgatgta gttgaccccg
     9721 atcgcctcgg ctttgatcag cacctggccc gggccggggg agggttgggg cttctcgacg
     9781 tagcggagga cctctggacc gccgtgttcg gcgacttcga ttgcgtgcat cccactatca
     9841 tcccggcaat gaagctcgcg cggccaaacg tgttccatcc gcgcatcgtg ctggccggat
     9901 gtcggcagct tgtcgacggt gacggcgacg acgcgggcct gattcccgcg ctgagtcgac
     9961 gcggcctgca cgcgcggtgg cggtcatggg acgatccgga cacgttgcgc gccgacgtcg
    10021 tgatcctgcg cgcgacgtgg gactacgccg agcggctcga cgagttcgtg gcgtggacga
    10081 cggcggtgcg tcacctgctc aacgcacccg cggtggtcgc ctggaacgcg gacaagcgct
    10141 acctgctcga cgtggagcgg cgcggcgtgc cgatcgtgcc gaccgaggtg ttcggacctt
    10201 ctgatcgggt gcgcctgcca gcgggggagg tcgtcgtcaa accatcgata ggcgtgggtt
    10261 cggtcgacgc gcaacggttt acggaccggg ccgcggccca agcccacgcc gccgcgctgc
    10321 aggagcaagg ccgcagcgtg ctggtgcagc cctacgacga ccgggtcgag cgcgccgggg
    10381 agacggcgct ggtgttcctc ggcggcaagc cgtcacacgc gttcaccaag gccgcgatgc
    10441 tgcctccgca aggacagcgc atcgaactgg attccaccgg aacgtacgca atcgagactc
    10501 tcgcccccgc cgacccggac ttcgaaatct gggacgtcgg gttcgcggcg ctggacgcgg
    10561 ccgcccagca gctggacatc gccgccggcg acttcctgta cgcgcgagtc gacgtcatcg
    10621 gcggcatcga cgatccccgg ctgctggagc tggagctcat cgagccgtcg ttgggctgga
    10681 cacagctcga cgaacgaacg cgtgaccttc agcagcggga gttcgcgcga cgcctgtcgt
    10741 cagcgttgga gcggctcggg cttggtccgc tctcgcatcg acgcccatag cgcggcggtg
    10801 gcggccgtca cggcggcggc gccggcgacg tggaccgcga ccaagaccgc cggtactcct
    10861 gcgaagtact gcaccgtccc gaccaggcct tgggcgacga cgagcgcgat cagcacggcg
    10921 aggcgcagca gcaccggccg cggcgcggac accgcgagca ggccgaagcc caagccgacc
    10981 agcagcgcga gatagccgat cagcagcgac gagtgcgcgt gcaccagcgt ggtgatctcg
    11041 attgccagcc gcggcaccgt gcgggtgggg ctggtgtcgc cggcgtgcgg tccggatgcg
    11101 gtcaccagcg tgccggtgac caggaccgcc gcgagtgcga cggcgctcag aacgaccagc
    11161 cagcgcaacg gctttggcgc caccagccgg ggcacaccat cgtcgggctc accgaccttc
    11221 acgaacagca gcacggcgag ccacaccatc gtcatcgacg cgagcaggtg cagcgcaacc
    11281 gtccaccaca agagcccggt cagcacggtg atgccgccga tgacggcctg cacgaccgtc
    11341 gacgccggca tcagccacgc gtacacctga acctcggcgc gacgacgggc ccgggtgacg
    11401 gccaacacgg ccagcgcggc ggtcagcacc accaggaacg tcaccatccg gttgccgaac
    11461 tccaccgcct ggtggatgcg cggaacctcg gacacggcga ccggggtgaa gctgcccggg
    11521 aagcactgcg gccacgtcgg gcagccgagg ccggacgcgg tgacgcggac gatcgcaccg
    11581 gtgaccgcga tgccgccctg cgtcaggatc actgcggcgg caatcagacg ctgggtgcgc
    11641 aggctgggca tcggcaacac gtcgaccagc cgcagcagga accgcataga cgaatcttaa
    11701 ctacagcttg tagtagctca gttttgcgcc gggagcgacg ttttgcaggc cgtcactcgc
    11761 actctcgctg cgaaacgtga ctctcgcgga tcagtaccag atggcgacca gctgccggct
    11821 ctcgttgagt tgaggcgggt cgggcagcga cgtggcttcc cgccgcgaga tgcggactgc
    11881 actccaggcc ggagttcgac tccgactcat cccgaggggc aggcgtcgac gccgagagta
    11941 actgtcacgt gaagcggaac agccgcagcg ccgccagccc ggccagcgtg ccccacaccg
    12001 cgagcacgca caggccgaac cagtcgacgg acaccgtcat cgcctgcgac aaagcctcgg
    12061 tgagcgcgcc cgacggcgtc agccgcgcca cccactcgac gccgcgcggg accaacccgc
    12121 cacccaacgt caacgccccg aaaccggcga acacgaacca catcaggttg gcaagagcca
    12181 gcacgacctc cgccttcagc gtcccgccca gcagcaggcc gagagcggcg aaagccgcgg
    12241 tgcccagggc gatcacgacc gcgcccagca tcaggccggt cagctgaggc cgccacccga
    12301 gaccaaagcc gatgccgccc aggatgattg cctgcaggaa gacgaccgta accacggcaa
    12361 gcgacttgcc cgcgatgatg ccccacaccg gcagcggcgt cgcaccaagc cgtttcaacg
    12421 cgccgtagcg ccggtcgaat gccaccgcga tcgcctgccc ggtgaacgcg gtcgagatca
    12481 ccgctagcgc catgatcgcc ggcacgaacg tggccgcccg gttgtcgccg aacgaaccca
    12541 gcggcaacag cgtcagcccg accagcagcg tgacggggat gaacagcgtg agcagcagct
    12601 gctctccgtt gcgcagcagc agtttcagtt cgagaccgta ctgggcggcg agcatccgct
    12661 gcacggtgtt aggccgcggg tcgggcgtga aagtctctgg cgcaaagcgt gtttcggtca
    12721 cctaacctcc ctgccggtga gttccaggaa tacgtcttcg aggctgcgct gttcgacgcg
    12781 catgtcggtg gccagcacgt cgagccgagc gcaccacgcc gtcactgtcg cgagcacttg
    12841 aggattgatc ggcccctcga ccaggtactg gccggacgac acctcggtgg ccttgtaccc
    12901 ctccggcaac gccgccgaca gcagcgacaa gtcgagcatc cgaggcgcgg tgaaccgcag
    12961 ctggttctcc gcgccgttgc gcatgatttc ggccggggtg ccggcggcca ccgcgacacc
    13021 gcggtcgatg atcaccagcc ggtccgccaa ctgctcggcc tcggtgagtt gatgtgtggt
    13081 cagcacgacg gtcacgccgt cgcggcgcag cgcgtcgatc agttcccaca ccaccagacg
    13141 ggcgtgcgcg tccaagccgg ccgtcggctc gtcgaggaac accagctccg ggcgcccgac
    13201 gagtgcgcac gccaatgcga gccgctgctg ctgaccgccc gacagccgtc ggtaggtggt
    13261 gcgcgcgacg tcggtcagcc cgagcgtgtc catcaaccac ttcggatcca gcgggttagc
    13321 cgcatacgcc gcaacgagat tcagcatctc gccggcccgc gccgcgggat agccgccgcc
    13381 gccctgcagc atcacgccga tccgttcccg cacgcgggcg ttgtcggtga cggggttcat
    13441 gccgagcacc tcgatggtgc cggcgtctgg ccggacgaac ccttcgcaca tctccacggt
    13501 cgtggtcttg cccgcaccgt tggggcccag caacgcaaac acttcggcgc gttggacgtc
    13561 gaggtcgagg tcggacaccg cggtcgtcga cccgtaccgt ttacacaccc cgcgcagtcg
    13621 cacggggatt ggagcggtcg aagtcacggg gactcagcgt atgcgccggg caccgggctc
    13681 gttgtcggcg ccggggatat cggcttcggg acatcgggct cggccgcttc ctcgctgccc
    13741 gtgacgggat gcgggacgct gcgccacggc aacatcgagt aggtgagggc gatcaggatc
    13801 agcacgatca cggcgctggc cacggtcgcg tcgatgatct gaaacaacgc gaaccggtcg
    13861 ccgttggccg tgggtccgaa aatgccgacg acgagcgtga cgatgatcgt cgcgatgcgg
    13921 aagcgaggcc gggtggccca ggccgccagc gggatgatcg cccacagcag gtaccacggc
    13981 tgcaccacgg ggaacagcag gaccgtcgcg ccgagcgcga cgcccaggcc gccgatcggg
    14041 tgcaaccggc cgcgcagcac cgcgatcagc agccacgaca ccaagatcgc gatgatcagc
    14101 acgccgatgg cacgagtcag ccccagcacc gcggtggtgt gatcgcccag gcccagcagg
    14161 attccgacct gcccggtgcc cagcgccaac aacgtcgggg gtgacatcca actccgcacg
    14221 acattcgcgg tgcccagggt gttgacccag ccgaagccca ggccgctggc caacccgatg
    14281 gccgccgtca ccgccagcga catcaagccc agcagtccac ccgccgcgat cacgcctcgg
    14341 atgctggcgc cccatcgccg cgcgagcgcc atcgcgacga agcccagcgc cagcagcgac
    14401 ggaagcttca cctgcgagga caaagtgatc agcaccacgc ccgccaccaa catcgccggc
    14461 gggtaccaag gctcccagtc ggcgcggtga cgaggccatc gcagcggacg gggcagcagc
    14521 gcacgcgtgg cgtcgactcc gcgcagcgcg aactcggtgc cggccagcat caggccgagc
    14581 atcagcgcct cgttatgaat cccggcgacc aggtgcatga tcagcagcgg gttggtggcg
    14641 cccagccaca gcgcgctgac ctcggcgacc ccgcaccggc gggcaaggcg tggcgtcgcc
    14701 cagacgatca aacccacgcc gatcagcacc accaggcggt gacacagcac ggccgcgacg
    14761 atgttttcgc cggtgagcga cgaaatcccg cgaccgatcc agaggaagag cgggccgtac
    14821 ggcgcggggg tctcacgcca caggctgggc accgacagcg tgaagacgtg gtcgagtccc
    14881 agggccggcg ccgggccgac gcgatacggg tcgaggccga gccgggaaat ctcgctttgc
    14941 gccaggtagg agtagacgtc cttgctgtac atcggcggtg cgatcagcag agggaggatc
    15001 cacagcatca gcgttcggtc gagctggctg cgtgacatcc ggcgctcgcc gagcgcgaag
    15061 cggcccagca tcagccacgc gagtgccatc atcaccgcgc cggtggtggt catcgtcagc
    15121 gacacggtct gcatgcgcga cggcaggttg agcagccgga ccccgaacgt cgggtcctgc
    15181 acgacgggcc gcgcgccggc gcccagcgag ccgatggcca tcaggaccgt gccggtggcg
    15241 ccgaagagcc gggtccgccg cagggccacg acctcggcgt cgttgagggg tgagccgaca
    15301 gtccgctcgt cgccgtgcca gcgggcaatc gacaagctca gggagccatg gcggcgggct
    15361 gccatcccag cagcgtaacg gcccacgccg gtgtccaatc tcaccgcgac cgttagggtg
    15421 cccttcgtag accttgtcgt cgaattccgt cacaatggtg ttgtgaaaat ccgtccggaa
    15481 actgaccgcc acacccgtgg cgagatagtc cagttattgc tggaggccgg acccatcacc
    15541 gccggggcga tcggcgagcg gctcggtatc tcggccgccg gcgtgcgcag gcacttggac
    15601 gcattgctgg acgctgggga cgcgcaggcc ggcccggcgg cggcgtggca gcagcatggc
    15661 cgcggtcggc ccgcgaagcg ctaccggctg accccggcgg ggcgggccaa actaggccac
    15721 acgtacgacg acctggcggc ggccgcgatg cggcaactgc gtgaaatcgg cggcgacgag
    15781 gcggtgcgca cgttcgcgcg ccgccgcatc gacacgatcc tggcgggagt aggtgacggg
    15841 cccgacgacg ttgaagcgac tgcggagcgc gtcgccgcgg cgttgacgaa agccggctac
    15901 gcgacgacga cgacgcgggt gcacggccag gtcgacggca tccagatttg ccagcatcac
    15961 tgcccggtgt cggacgtcgc ggaggagttc ccggagctgt gccagaccga acgggaggcg
    16021 ttcgcggaga tcctcggcac gcacgtgcag aggctggcga cgatcgtcaa cggagactgc
    16081 gcctgcacca cccacgtgcc actgactgac ccttcagcgc acagcccgcg cggggggaag
    16141 acgaaactcg aaggagcctc gatatgacgg ctacgccgga cacgtcgacc cacaagattg
    16201 gcgagccgct cagccaggac gaggcgatcg cctcgctgag ccggtacggg tacggctggg
    16261 tcgactccga cgacgcgggc gccagcgcca agcgcgggct gtccgaggac gtcgtgcgcg
    16321 atatctcggc caagaagaat gagccggagt ggatgctgca gcagaggctg aaggcgctgc
    16381 gcatcttccg ccgcaagccg atgccgacgt ggggctccaa cctcgagggc atcgacttcg
    16441 acaacatcaa gtactttgtg cgctccaccg aaaagcaagc ggcctcttgg gacgatttgc
    16501 cggacgacat caaaaacacg tacgacaggc tcggaattcc ggaagctgaa aagcaaagat
    16561 tagttgctgg agtagcggct caatacgaat ccgaggttgt ttatcaccag ataagagagg
    16621 atttagaggc ccaaggtgtc atattcttag atactgatac tggtttgcga gagcatccgg
    16681 atatcttcaa gcagtacttc gggacagtaa ttccagcagg tgataataag ttttctgcac
    16741 tgaacacctc agtctggagt ggtgggagtt tcatttatgt gccgccgggc gtgcgtgtcg
    16801 acatcccatt gcaggcttat ttccgcatta acaccgagaa tatgggtcaa tttgagcgaa
    16861 ctctgatcat cgccgacgag ggtgcctacg tgcactacgt cgagggctgc ctaccggccg
    16921 gtgaactcgt cacgatggct gacggcaacc tccgtccgat cgactcgatt cgcgtcggcg
    16981 actccgtcat gagccacgac ggcaagcctc atcgcgtcaa cgcgattcag gtgcgagacc
    17041 tgaacggtga actgttcacc ttcacgccga tgtcccccgc caacgcgttc tctgtgacgg
    17101 ctgagcaccc actcctcgtc gttccacgcg aggaggtgcg ggttgcgcgg aaggcgcgca
    17161 atggatggaa ggcggaggtc gacaccgcga agttgcgctg tgccgagccg cgctggatcg
    17221 cggccaagga tgtcgccgag ggcgacttcc tggtctaccc gaagcccaag ccgatcccgc
    17281 acaagacagt tctgccgctc gagttcgcgc ggctggccgg ctactacttg gccgagggac
    17341 atgcaacgct gacgaacggc tgtgagtcgc tgatcttctc gttccacagc gacgagttcg
    17401 attacgtcga agacgtacgc caggcgtgca agtcgctgta cgagacggca ggctcggtgt
    17461 tggtcgagga gcacaagcat tccgctcggg tcaccgtcta caccaaggcc ggctacgcgg
    17521 cgatgcgtga caacgtcggc gtcgggtccg cgaacaagag gctgtcggat ctattgatgc
    17581 gccaggacga gacgttcctg cgcgagctgg tcgacgccta tgtgaacggt gacggcaacg
    17641 tcatccggcg cggtggtgcg ctgtggaagc gcgtgcacac cacgtcacgc gtctgggcat
    17701 tccagttgca gtcaatcctt gcgcgccttg gccactacgc gacggtcgag caccgcaagg
    17761 atctgtacca ggtgcagtgg acggaaggtg gcagcggccc gaagcaggcc cgcgactgcg
    17821 gcgactactt cgccgtgccg atcaagaagc gcgcggtccg cgaagcgcac gagccggtgt
    17881 tcaacctcga cgtcgagcat ccggacagct acctggccta tggcttcgcg gtgcacaact
    17941 gcacggcgcc gatctacaag tccgattcct tgcactcggc cgtggtcgaa atcgttgtca
    18001 agccgcacgc ccgggttcgg tacaccacaa tccagaactg gtcgaacaac gtctacaacc
    18061 tggtcaccaa gcgcgcccgc gcggaggccg gcgccacgat ggagtggatc gacggcaaca
    18121 tcggctccaa ggtcaccatg aagtacccgg cggtgtacat ggtgggcgag cacgccaagg
    18181 gcgaggtgct ctcggtcgcg atggccggcg agggccagca tcaggacacc ggcgcgaaaa
    18241 tgcttcacct ggcgccgaat acgtcgagca acatcgtgtc caagtcggtg gcccgcggtg
    18301 gcggccgtgc ctcgtaccgc ggcctggtgc aggtcaacaa gggcgcgcac ggctctcgtt
    18361 ccagcgtgaa atgtgatgcg ctgctggtgg atacgatcag ccgcagcgac acctacccgt
    18421 atgtcgacat ccgcgaggac gacgtcacga tgggccacga ggccaccgtg tccaaggtca
    18481 gcgaggacca gctgttctac ctgatgagcc gcggcctcac cgaggatgag gcgatggcga
    18541 tggtggtgcg cgggttcgtc gagccgatcg ccaaggaact gccgatggag tacgcgctgg
    18601 agctcaacag gctgatcgag ctgcagatgg aaggtgcggt cggctagtga ccaacgtgac
    18661 tgaagcggtt gagggttcag ccctttccgc cgccaactcc ggctcaacgt tggctggcgc
    18721 caacaagggc gagatcttca cctcgttcga cgtcaacgca ttcgaggtgc cgggtggccg
    18781 cgacgagatc tggcggttca ccccgctccg ccggctaagc ggtctgcacg acggatcggc
    18841 accggccacc ggaagcgccc acatcgaggt cgcagagcgg ccaggcgtga cggtcgaaac
    18901 ggtgcgacga ggcgacgaac ggctcggcca aggcggcgca cccgccgacc gcgttgccgc
    18961 gcaagcgttc tcgtcgttca acaaggcgac gatcgtgacc gtcgataaag gcgccacacc
    19021 cggcgagccg atcgagatca agctcaccgg ccccggcgaa ggcaagaccg catacggaca
    19081 cctgcaagtc cgcgtcggcg aactcgccga agcggtcgtc gtcatcgacc agcagggcag
    19141 tggaacctac gccgacaacg tcgaattcgt gctgggcgac gcctcacggc tcacggtggt
    19201 gtctgtcgcc gactgggccg acgacgccgt ccacgtcacc tcccaccacg ccgcgctcgg
    19261 gaaggacgcg gtgctcaggc acatcgctgt cacgctcggc ggcgacgtgg tcaggctgac
    19321 aggccgagtg aagttctgtg cgcccggggg cgacgctgag ctgctcgggc tctacttcgc
    19381 cgatgacggc cagcactttg agtcgcggct gctgatcgac cacgccgagc cgaactgccg
    19441 ttccaacgtg ctgtacaagg gtgcgctgca aggcgatccg gaatcgcaca aacccgacgc
    19501 gcatacggta tgggtcggcg atgcgctgat ccgcgccaat gccaccggca ccgacacgtt
    19561 cgaggtgaac cgcaacctgg tgctcaccga cggggcccgt gccgactcgg tgcccaacct
    19621 cgaaatcgag accggcgaaa tcatcggtgc cggacacgcc agcgccaccg gccgtttcga
    19681 cgacgagcag gtcttctacc tgcgctcccg cggtatcccg gaggaccaag caagaagact
    19741 gatcgtgcgg ggtttcttca acgagatcat cgccaagatc accgtgcctg ccattcgcga
    19801 acgcctcact gacgccatcg aatacgaact agctcttacc gaaggacgcc tatgacaact
    19861 cttgaaatca aggacctaca cgtcagtgtc accccgagcg agggcgagga aatcccgatc
    19921 ctcaagggcg tcgacctgac cgtgaagtcg ggcgaaatgc atgcgctgat gggacccaac
    19981 ggttctggca agtcgacgct gtcgtacgcg atcgcgggtc acccgaagta caaggtgacg
    20041 tcgggctcga tcacgctcga cggcgaggac gtgttggcga tgagcgtcga cgagcgcgcc
    20101 cgcgcaggcc tgttcctggc gatgcagtac ccggtcgagg tgcccggcgt gtcgatgtcg
    20161 aacttcctgc gcaccgcggc caccgcagta aggggcgagg cgccgaagct gcggcactgg
    20221 atcaaagagg tcaaggccgc gatggatgac ctcgacatcg acccggcatt ctccgaacgc
    20281 agtgtcaacg aagggttttc gggcggcgag aagaagcgtc acgagatcct gcagctcgac
    20341 ctgctgaagc cgaagatcgc gatcctcgac gagaccgact ccggcctcga cgtcgacgca
    20401 ctgcgagtgg tcagcgaggg cgtcaaccgg tacgccgagg agacccacgg cggccttctg
    20461 ctgatcaccc actacacccg catcctgcgc tacatcgagc cgcagttcgt gcacgtgttc
    20521 atcggtggtc gcatcatcga atccggtggc ccggaactgg ccgacgaact cgacgcgaac
    20581 gggtatgagc gcttcaccga gaaagccgcc gcgggggcat agcgatgacc acgtcggctc
    20641 gcactctgga catcgacgcg attcgcgccg acttcccgat actgaagcgg gtgatgcgcg
    20701 gcggaaagca gctggcgtac ttggactccg gtgccacgtc gcagcggccg gtgcaggtgc
    20761 tcgacgctga gcgggacttc ctgacgacgt cgaacggcgg ggtgcaccgc ggcgcgcatc
    20821 agctgatgga ggaggccacc gacgcctacg agcagggtcg cgccgatatc gcagcattcg
    20881 tgggtgccga cgccgacgag ctggttttca ctaagaacgc cacggaggca ctgaatctcg
    20941 tcgcgtacgt cctcggcgac aaccggttcg aaagggcggt cggaccgggc gacgtgatcg
    21001 tcaccaccga gctcgagcac cacgcaaatc tcgttccgtg gcaggagttg gcgcagcgca
    21061 cgggcgcgac gctgcagtgg tacagcgtga ccgacgacgg gcgcatcgac ctcgacagcc
    21121 tgcaactcga cgagcgggtg aaagtggtga cgttcagtca ccattcgaac gtcacgggtg
    21181 cgttggcgcc ggtgtccgag ctggtgtcgc gggcgcgggc cgtcggggcg ttgacggtgt
    21241 tggacgcgtg ccagtcggtg ccgcatcagc cggtggacct ccatgcactc gacgtcgact
    21301 atgcggcttt ctcgggccat aagatgttgg ggccgaacgg catcggtatg ctgtatggcc
    21361 gtcgtgagtt gttgaacgcg atgccgccgt tcatcacggg cgggtcgatg atcgagaccg
    21421 tgacgatgga ggccaccacc tacgcgccgg cgccgcagcg gttcgaggcc ggcacgccga
    21481 tgacgtcgca ggtggtcggg ttggccgccg cggcaaggta tttgcaggcg atcggcatgg
    21541 acacggtgga ggcgcacgag cgggaactgg tggctgcggc gctcgacggg ctgtcgggca
    21601 tcgagggtgt gcggatcatc gggccgacat cgttgacgga tcgcggctcg ccggtggcgt
    21661 tcgtcgtgga cggcgtgcac gcgcacgacg tgggccaggt gctcgacgat gacggtgtgg
    21721 cggtgcgggt ggggcatcac tgcgcgtggc cgctgcaccg gcgcttcggg atttcggcga
    21781 cggcgcgggc gtcgttcgcg gtgtacaaca cgttcgacga ggtggaccgg ctggtggccg
    21841 gcgtgcgtcg ggcggtggac ttcttcggcg ggccgtgagt tgcggctgga gcagatctac
    21901 caggaagtga tcctcgatca ctacaagcac ccacaccatc gcgggctgcg ggagccgtac
    21961 ggcgccgagg tttttcacgt gaacccgacg tgcggtgacg aggtcaagct gcgggtggcg
    22021 ctgagcgacg acggggaaag cgtggccgac gtgtcgtatg acggtcaggg ctgctcgatc
    22081 agccaggcgt cgacgtcggt gctcacggat caggtgattg gccagagcgt gggccgggca
    22141 ctcgacatcg tgggcgcgtt caccgagatg gtgtcgtcgc gtgggaccgt cgagggcgat
    22201 gaagaggtga tcggcgacgg gatcgcgctt gcgggggtct cgaagtatcc ggcgcgggtg
    22261 aaatgcgcgt tgctgggatg gatggctttt aaggatgcgc tggcacaggc cagcacgagg
    22321 atagatgtgc aggaggtgga cgatgagcga aacggccgct caattgccgg atgaggagtt
    22381 cctggccgac ctcgaggagg cgatgcgcga cgtcgtcgac cccgaactcg gcatcaacgt
    22441 cgtcgacctc ggcctggtgt acgggctcaa cgtcgaacgc ggcgaggacg gcacgaccgc
    22501 gctcatcgat atgacgctga cgtcggcggc gtgcccgctg accgacgtga tcgaggacca
    22561 atcacgaatg gcgttggtgg gcgccgggct ggtgaacgag atcaagatca actgggtgtg
    22621 gaatccgccg tggggcccgg acaagatcac cgaggacggc cgggaacagt tgcgggcgtt
    22681 gggattcacg gtctgagtac ggctcgtgcg cgagatcacg gcctccgagg cgtcacagaa
    22741 cttctccgcc gtcctcgact cggtcgggtg cggcgctgaa cgcagtgctc cggcgctgga
    22801 caggggtaga cgtcccctcc cacatgtccc agtcgatcgt gaagccgtgc cg
//
LOCUS       DASWBF010000526         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_908487,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000526 DASWBF010000000
VERSION     DASWBF010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908487"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..>2267
                     /locus_tag="VF874_19640"
     CDS             3..>2267
                     /locus_tag="VF874_19640"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX9178189.1"
                     /translation="MDAVEGHGTGTRLGDPIEAQALLATYGRDRLDGPLRLGSIKSNI
                     GHTSAAAGVAGVIKMVMAMRHGMLPRTLNVDEPSPYIDWDAGDVKLLVEAEEWSAGER
                     PRRAGVSSFGVSGTNGHVIIEEAPAEPVPVIDAVQSHRPAVVPVLVSGKSQTALAAQA
                     GRLRSYLLARPELDVADVAFSAVVTRALLDHRAAVVASDREGLLAGLGALAEQQPGAE
                     ALAGCLVPGKTAFLFTGQGAQRAGMGAGLAAAYPVFAQALEEVCAELDPKLGRSLKEL
                     LFAAGGSPEAALLDRTEFTQAGLFAVEVALFRLVGSLGVRPDFLIGHSVGELAAAHVA
                     GVLSLEDACTLVAARGRLMGGLPAGGGMVAVQATEAEVSESLNGFEGRLSVAAVNGPM
                     AVVVSGDVDAVEEWLPRWQDRKTTRLRVSHAFHSPRMEPMLAEFRSIAAGLTFREPRI
                     AVVSNLTGGVVSAELADPGYWVAHVRAAVRFADGVRTLERAGVTRFFELGPDAALTAL
                     ARQSLESVDDDSAVFVPVLRAGKPEAEVFAGFLGQAHIAGVAVDWSAFYAGSGAQRVE
                     LPTYAFQRERYWLSPGAGSGDPAAAGLGRIEHPLLAATVQVGDRDEWVFTGRLSTDTA
                     PWVRDHVVLGMVIVPGTALVELAVAAGRAVGSPVVEELVLEAPLTLHENTAVHLQVTI
                     GEPDEDGRRPVAIYSRPETRGDDEQREAICHARGLLTQDDNRVAASWMPAEWPPVDSE
                     PIAADALYARLTEIG"
BASE COUNT          273 a          614 c          931 g          449 t
ORIGIN      
        1 atgtggatgc ggtcgagggt catggtacgg gtacgcgttt gggtgatccg atcgaggcgc
       61 aggcgttgct ggcgacgtat gggcgcgacc ggttggatgg tccgctgcgt ctggggtcga
      121 ttaagtcgaa tatcggtcac acgtcggcgg cggccggtgt ggctggtgtg atcaagatgg
      181 tgatggcgat gcggcacggg atgctgccgc ggacactgaa tgtcgacgag ccgtcgccgt
      241 acatcgactg ggacgcgggt gacgtcaagc tgctggtcga ggccgaggag tggtcggctg
      301 gtgagcggcc gcgccgggcg ggtgtgtcgt cgttcggtgt cagtggcacc aacgggcacg
      361 tgatcatcga ggaggcgccg gcggagccgg tgccagtcat cgatgcggtg cagtcgcatc
      421 gcccggccgt ggtgccggtg ctggtgtcgg gcaagagcca gacggcgttg gctgcgcagg
      481 cgggccggct gcggtcgtat ttgctggcgc gtccggagct ggatgtggcc gatgtggcgt
      541 tctctgcggt ggtgacgcgg gcgctgttgg atcatcgtgc ggccgtggtg gcgtccgatc
      601 gggagggcct gcttgcgggt ttgggtgcgc tggccgaaca gcagccgggc gcggaagccc
      661 ttgcaggttg tttggtgccg ggtaagacgg cgttcttgtt cactggccaa ggtgcccagc
      721 gtgcgggtat gggtgcgggt ctggcggcgg cttatccggt gttcgcgcag gcgctggagg
      781 aggtttgtgc cgagctggac ccgaagctgg gccggtcgct gaaggagttg ctgttcgctg
      841 ctggggggtc gccggaggcg gctctgctgg atcggacgga gttcacgcag gcggggttgt
      901 tcgctgtcga ggtcgctttg ttccggctgg tcggatccct cggtgtcagg ccggatttcc
      961 tgatcgggca ttcggttggt gagttggctg ctgcgcatgt ggccggggtg ttgtcgcttg
     1021 aggatgcgtg cacgctggtc gccgcccgcg gtcgtttgat gggtggattg ccggccggtg
     1081 gtgggatggt cgcggtgcag gcgaccgagg ccgaagtctc cgagtcgttg aacggtttcg
     1141 aaggccggtt gtcggttgcg gcggtcaatg gcccgatggc ggttgtggtg tcgggtgatg
     1201 tggatgcggt cgaggagtgg ttgccgcggt ggcaggaccg caagacgacg cgtttgcggg
     1261 tgagtcatgc gttccattcg ccgcggatgg aaccgatgct ggccgagttc cggtctatcg
     1321 cagcgggttt gacgttccgt gagccgcgga ttgcggtggt gtcgaacctg accggtggtg
     1381 tggtgtctgc cgagctggcc gatccgggtt actgggtcgc tcacgtgcgt gcggcggtcc
     1441 ggttcgctga tggggtgcgc accctcgagc gtgcgggtgt gacccggttc ttcgagctcg
     1501 ggccggatgc ggcgttgacg gcgctggcgc ggcaatcgct ggaatcggtg gatgatgaca
     1561 gcgcggtgtt cgtgccggtg ctgcgggcgg gcaagccgga agcggaggtg ttcgccggct
     1621 ttctgggtca ggcgcatatc gctggtgtgg cggtggattg gtccgcgttc tacgccggtt
     1681 cgggtgcgca gcgggtcgag ctaccgacgt atgcgttcca gcgagagcgt tactggctgt
     1741 caccgggtgc cgggtccggt gatccggcag cggccgggct gggccggatc gagcacccgc
     1801 tgctagcggc cactgtccag gttggtgacc gcgacgagtg ggtgttcacc ggccgcctgt
     1861 ccaccgacac cgccccgtgg gtacgggacc acgtggtgtt gggcatggtg atcgtccccg
     1921 gcaccgcact ggtcgagctc gcggtcgcgg ccggccgcgc agtgggcagc ccggtggtcg
     1981 aggagctggt gctggaggcg ccgctgaccc tgcacgagaa caccgcggtg cacctgcagg
     2041 tcaccatcgg cgagccggac gaggacggcc gccgcccggt cgcgatctac tcccgccccg
     2101 agactcgcgg tgacgacgag cagcgcgagg cgatctgcca cgcacgcggc ttgctcactc
     2161 aggacgacaa ccgggtcgcc gcgtcgtgga tgccggccga gtggccgccc gtggattcgg
     2221 aaccgattgc cgcggatgcg ctctatgcgc ggctgaccga gatcgga
//
LOCUS       DASWBF010000527        12613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_261763,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000527 DASWBF010000000
VERSION     DASWBF010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261763"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF874_19645"
     CDS             <1..211
                     /locus_tag="VF874_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178190.1"
                     /translation="GGTAGRGGADMSGAGEQDRGGLTDEERALTMRRWELDVEYDVAE
                     NITDPALRANALIRAKHCGKATKGK"
     gene            complement(270..416)
                     /locus_tag="VF874_19650"
     CDS             complement(270..416)
                     /locus_tag="VF874_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178191.1"
                     /translation="MTRSEQTRLARGARLGCRPSSRTGLAWLGYCAWVIHIELDKTAK
                     IIES"
     gene            complement(519..592)
                     /locus_tag="VF874_19655"
     tRNA            complement(519..592)
                     /locus_tag="VF874_19655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(556..558),aa:Leu,seq:taa)
     gene            1063..1371
                     /locus_tag="VF874_19660"
     CDS             1063..1371
                     /locus_tag="VF874_19660"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX9178192.1"
                     /translation="MKSKIGMVAPAAAALTAAALAWAPAAIASPDDFLSELSTNGVSL
                     PGLTPSQTVAAGYQACSALKSGTSVLDEMSAVEQKYGFSQGTLFVSAATTNLCPDFAG
                     "
     gene            complement(1378..2238)
                     /locus_tag="VF874_19665"
     CDS             complement(1378..2238)
                     /locus_tag="VF874_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX9178193.1"
                     /translation="MCRLFGLHAGRSVVTGTFWLLDAPDSLMAQSKRNPDGTGLGVFG
                     PDGAPQVRKEPIAAWEDTEFASEAHEMSGTTFIAHVRYASTGSLDARNTHPFLQDDRL
                     FAHNGVVAGLDVLDGRLAELSVADLVVGQTDSERVFALITGAVRARSGDVAAGFADAM
                     TWLAANVPIYAANLMLATATDMWALRYPETHELFMLDRRDYSTAFEMQTHRIHARSKD
                     LDEHASVLFASEPMDDDDWRLLESGVAVHVDADLSITQRELLPHPPKHQLALSDLSSQ
                     AAGSQHPAAR"
     gene            2268..2972
                     /locus_tag="VF874_19670"
     CDS             2268..2972
                     /locus_tag="VF874_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HEX9178194.1"
                     /translation="MTARNVIAAAIASLAMLTGAAGNAVAEPVEGTEPSNVGDAKTAA
                     VAYHDSGQYQHDLAVIAAQAINWIGTEVPTTPRAAIVFDIDETALSNWEVIRANDFGR
                     FINGPCNLPDACAWRAWDETAQSTVIAPTLDTYNTAKSLGAAVFFITGRDEGQRAATE
                     QNLQSAGYTGYTRLIMPAPGAQYASAADFKAPQRTAIEDEGYTIVANIGDQPSDLAGG
                     HSQQTFLLPEPFYRIP"
     gene            complement(3200..4135)
                     /locus_tag="VF874_19675"
     CDS             complement(3200..4135)
                     /locus_tag="VF874_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX9178195.1"
                     /translation="MRVGAIDCGTNSIRLLIAEVADGGLHELHREMRIVRLGQGVDAT
                     GEFAPEAIARTRAALTDYTELLARLQVPKVRMVATSAARDAANRQEFFAMTAEVLAAA
                     VPGAVAEVITGEEEAALSFHGAVAELDCAEAPFLVVDLGGGSTELVLGTDDVDASFSA
                     DVGCVRLTERCLHSDPPTATEVAAAREVVRERLGEALRVVPVDRAHAWVGVAGTMTTL
                     SALAQKMTRYDSEAIHLSRVGFPELLGVCEGLIAMTHAQRAVLGPMHEGRVDVIAGGA
                     IVVEELAYALRQRAGIDQLIVSEHDILDGIALSIG"
     gene            complement(4132..4623)
                     /locus_tag="VF874_19680"
     CDS             complement(4132..4623)
                     /locus_tag="VF874_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX9178196.1"
                     /translation="MVDPADLDAVARQLGREPRGVLAIAYRCPNGEPGVVKTAPRLPD
                     GTPFPTLYYLTHPALTAAASRLESSGLMKEMTTRLSAEPELAAAYRRAHESYLAERDA
                     VEPLGTTFSGGGMPDRVKCLHVLIAHSLAKGRGVNPLGDEALALLATDPTMDGLLVKE
                     DWT"
     gene            complement(4616..5275)
                     /locus_tag="VF874_19685"
     CDS             complement(4616..5275)
                     /locus_tag="VF874_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX9178197.1"
                     /translation="MPDAKRPDPKRRSPGSRPSRGSESVRERPRSTAPRREPRAAEQR
                     PAEEPVTESITAQIAAAAARQSEQRLGFTARRAAILAAVICVLTLTIAGPVRTYFAQR
                     TEMKQLAASEAQLRTQIAELEQQKAKLGDPVFIAAQARERLGFVMPGDVPYQVQLPPG
                     AVTPSDQPGASSSTTSSNDPWYTALWRTIADTPHNIPSPAAPPPAPPPPPGPPPPPPG
                     G"
     gene            complement(5279..6568)
                     /gene="eno"
                     /locus_tag="VF874_19690"
     CDS             complement(5279..6568)
                     /gene="eno"
                     /locus_tag="VF874_19690"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9178198.1"
                     /translation="MPIIEQVGAREILDSRGNPTVEVELALTDGTFARAAVPSGASTG
                     EHEAVELRDGASRYGGKGVEKAVNAVLDEIAPAVIGMSANEQRLVDQALLDLDGTPDK
                     SRLGANAILGVSLAVAKAAADSAALPLFRYIGGPNAHILPVPMLNILNGGAHADTGVD
                     VQEFMIAPIGAPSFKEALRWGAEVYHALKSVLKKQGLSTGLGDEGGFAPDVAGTKAAL
                     DLILSAIESAGYKPGSEVALALDVAATEFFSTDTGYTFEKETRDSAQMTAFYAGLLDT
                     YPLVSIEDPLSEDDWDGWVALTKEIGDRVQLVGDDLFVTNPERLEEGIEKGAGNALLV
                     KVNQIGTLTETLDAVALAHYSGYRTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPAR
                     SERVAKYNQLLRIEETLGDAARYSGDLAFPRFALEAD"
     gene            complement(6617..7348)
                     /locus_tag="VF874_19695"
     CDS             complement(6617..7348)
                     /locus_tag="VF874_19695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX9178199.1"
                     /translation="MTRVRWVRAVAVAAVTAVLMASSCSWHLGTPIPEGVPPPAGDPV
                     PEIDTHAAGRPAGRLHAWADARAPALNMPVNALEAYAYAARVAEVENPDCHLAWTTLA
                     GIGMVESHNGTYRGAAIAPNGDVTPLIRGVQLDGSHGNVEIMDSDGGLLDGDRGIDRA
                     MGPMQFIPETWRLYGVDANNDGVVSPDNFDDAALSAAGYLCWRGGDLATPEGWMKALR
                     AYNLSDQYARTVRDWATAYADGRPL"
     gene            7567..8523
                     /gene="efeU"
                     /locus_tag="VF874_19700"
     CDS             7567..8523
                     /gene="efeU"
                     /locus_tag="VF874_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HEX9178200.1"
                     /translation="MTAGADFTSVTAAGPTLSSQLLGSGLIGLREGLEAGIVVMILVA
                     FLVKAQRRDALKWVWLGVAAAVAMTIAVFLFIQYSAYTVTDLGAEAIAGIASLIAVAI
                     VTTMVLWMKKAAAGMSGELRAEMAKALETGSLAVLTLAFLAVGREGFETALFMVGYAE
                     AETVWPLVGLLIGVLLAAGIAWGMYMGAVRINLAKFFTYTGIFLIFVAAGILGYGVHA
                     LQTVGWLPGLGAKAFDITGWMNWSAWYGELIQGIFNITPTPTVLQLIVYLTYLVVVLA
                     VFLRPNRAPARPTGGAESSASADGAAPSAPSASQPTPERSTT"
     gene            8520..9683
                     /gene="efeO"
                     /locus_tag="VF874_19705"
     CDS             8520..9683
                     /gene="efeO"
                     /locus_tag="VF874_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEX9178201.1"
                     /translation="MNVPLPRSLVAAVAAVLAGVSLVSCAAKESDNNSSASGAATEVT
                     VNATDTACELSKTEAKTGTTNFVITNNGTKVTEFYVYGEGERVMGEVENISPGLQRKL
                     VVTLNEPGTFQTACKPGMIGDGIRGDFTVTGDAVTTNAEGKFQVASENYKQYVIGQTD
                     ALVTAVEAFVAAIKARDIASAKTQYPTSRVYYERIEPVAESFPDDLDPRIDLREADLE
                     PGQKWTGFHRLEKDLWVTGLQPDTNAIADQLVADVRELNQKVKSPDYTIDSTQIAGGA
                     QGLLDEIAISKITGEEDIFSHTDLWDFNANVEGSQAAVGSVRPILDERNAELGKRVDQ
                     RFAEVFALLDRYRQGDGFVLYDTVTEPQRQELSRAIDALSKEVSQVQGVIAPQ"
     gene            9664..10989
                     /gene="efeB"
                     /locus_tag="VF874_19710"
     CDS             9664..10989
                     /gene="efeB"
                     /locus_tag="VF874_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HEX9178202.1"
                     /translation="MSSPPNSEQLEDDSPTPERGGISRRKLFGAAGVTAAVVGAAGAG
                     ALAGRASAAAADDSTEIHRPIRFRGERQAGIVTPQQDRMHFCAFDITTDKRDDVVAML
                     REWTRMAERMAAGHETVPDGAVGLNPYAPPADTGEALGLPASALTLTIGFGPSFFLKD
                     GKDRFGIADRKPAALVDLPKFPNETMDPARCGGDICVQACANDPQVAVHAIRNLARVA
                     FGTAAVRYSQLGFGRTSSTTRDQSTPRNLFGFKDGTRNIKAEDTDKLHQHVWVAAGDG
                     PDWLTGGTYLVARRIRMRIENWDRTALLEQERVIGRHKGSGAPNGYPDEFDELDLDSK
                     NGDGDPLIDVDAHVRLASAEQLGGIEILRRGYNFTDGSDGFGHLDAGLFFLAFVRNPL
                     TQFVPMQAQLASSDALNEYITHTGSAVFACPPGMRENDSSAYWGSGLFG"
     gene            complement(11511..12236)
                     /locus_tag="VF874_19715"
     CDS             complement(11511..12236)
                     /locus_tag="VF874_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9178203.1"
                     /translation="MPPASNPRTAIVTGGASGIGAAIVHRLITDGYQVATLDITPAEG
                     GQSYTVDVTDRAQIDNALADVRSRFGPVTILVNAAGAEGSARFLDISFAEWQRVIDVT
                     LNGVFHTTQAVLPDMLDAGWGRIVNISSSSTHSGTPMKTHYVAAKSGVNGLTKSLALE
                     YGPKGITVNAVPPGFVDTPMLRNAAQRGDFGRNTLDDLVAATPVRRIGRPEDIAATCA
                     FLVSDEAGYITGQIIGVNGGRNT"
     gene            complement(12265..>12613)
                     /locus_tag="VF874_19720"
     CDS             complement(12265..>12613)
                     /locus_tag="VF874_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9178204.1"
                     /translation="ERQRSARARAEVMVTGYADIVSRNRTLAAVTTFDPSVRCVLRNQ
                     PEWSAVIDRQLALLAGADSSVSGSINAAVVMTGLAGGASTAPHRIDDDTLRAHLIEVG
                     RRTLGLPEPPPHR"
BASE COUNT         2120 a         4166 c         4203 g         2124 t
ORIGIN      
        1 cggcgggact gcgggccgag gcggtgccga catgagcggc gccggcgaac aggaccgcgg
       61 cggactgacc gatgaagaac gagccctgac gatgcgccgg tgggagctgg acgtcgagta
      121 cgacgtcgcc gagaacatca ccgaccctgc gctgcgcgcg aatgcgctca tccgagccaa
      181 gcattgcggc aaggcgacta agggcaagta gcccactcac cgatcgctct taccccacgg
      241 ccaaggcggc cacttggcgc ccatttaccc taggactcaa tgattttcgc ggttttgtcg
      301 agctcgatgt ggatcaccca cgcgcaataa cccaaccaag ccagcccggt tcggctcgaa
      361 ggtcggcagc caaggcgtgc cccgcgcgcc agccgggttt gctcgctgcg ggtcatggtc
      421 gcagagtagg tcgcgccaac gcaggggtga cgccggggtg aaccgccctg ccgtgaccgc
      481 cagccaccac cggaacatgc ttgacccgca tagatacgtg cccccagtcg gactcgaacc
      541 gacactggac ggattttaag tccgctgcct ctgccaattg ggctatgggg gcattgcagc
      601 cacgaagcct agccggtcag gaagatcgat taaattggcg atcttcccat cctgccgccc
      661 ggctttgact tcaccgaccg tgtgtgccct tccgttgcag gccattgcgg aacttctcgg
      721 cgtgccgcga gagcaccgcg ccacgctgtc cgatggtcga acaagattat cggcgaacag
      781 gacccggagt attcgagctg caacgcactc ggcgctgccg ccgaattagc cgggcacgca
      841 ttgtaattgg ctgagaagaa ggccaagcaa ctcggtacga catcgtcaca atgacgatcg
      901 acgtcttcgt tgtcatgctg gccatcaccg acaacgatga tcgtaagcgc aatcagagaa
      961 ttcaccgatt tgctgcggca ccggctgaga aggtggacga ttcttaggtc ggggcgaaga
     1021 agccgtctgt gtaccgcagt ccgcagagcc gaaaggacaa caatgaaatc gaaaatagga
     1081 atggttgcac cggccgcagc cgcattgacc gctgcggcgc tggcctgggc gcccgcagct
     1141 atcgcgagcc ccgatgactt tctcagcgaa ctcagcacga acggtgtctc cttgcccgga
     1201 ttgacgccga gccagacggt tgccgccggc tatcaggcgt gctctgcgct caagagcggg
     1261 acctccgtcc tcgacgagat gtccgcggtg gagcagaagt acggtttcag ccagggcacc
     1321 cttttcgtga gcgcggcgac gacgaacctc tgcccagatt tcgcgggctg aaccggttta
     1381 tcgagccgcg gggtgctgcg accccgctgc ctgagaactc aagtccgaca acgccagctg
     1441 atgcttcggc gggtgaggca gtagttcgcg ctgcgtgatc gacagatccg cgtcgacgtg
     1501 caccgccact cccgactcga gcagccgcca gtcgtcgtcg tccatcggct cgctcgcgaa
     1561 cagcaccgac gcgtgctcgt ccaagtcctt tgaccgcgcg tggattcggt gggtctgcat
     1621 ctcgaatgct gttgagtagt cacgtctgtc gagcatgaac aattcgtgcg tctccggata
     1681 tcgcaaagcc cacatgtcag tcgccgtggc caacattaga tttgccgcgt agatcgggac
     1741 gttcgccgcg agccacgtca tcgcgtcagc gaatcccgcc gcaacgtcac cgctgcgggc
     1801 tcgcaccgcg ccggtaatga gtgcgaacac ccgctcgcta tcggtctgtc ccacaaccag
     1861 atcggcgacg gacagctcgg ccagccgccc gtcgagcaca tcgagcccgg ccacgacgcc
     1921 gttgtgcgcg aacaaccgat cgtcctgcag aaacgggtgg gtgtttcgcg cgtccaggct
     1981 gccggtcgat gcgtagcgca cgtgcgcgat gaacgtcgtc ccgctcatct catgcgcttc
     2041 gctcgcgaac tcggtgtcct cccatgccgc tatcggctcc ttgcgcacct gcggtgcgcc
     2101 gtcgggtccg aacacgccca gccccgtccc gtccgggttc ctcttgctct gggccatcag
     2161 gctgtccggc gcgtccagca accagaatgt ccccgtcacc acggagcgcc cggcgtgtaa
     2221 gccgaacagt cggcacatac gtcgacgcta ccgtttaatg tcgctgcatg accgctcgga
     2281 acgtcatcgc agctgccatc gcctcgctcg ccatgctcac cggagcggcc ggaaatgcgg
     2341 tcgccgaacc cgtcgaaggc acagagccct ccaacgtcgg cgacgccaag accgccgccg
     2401 tcgcctacca cgacagcggt cagtatcaac acgacctcgc ggtgatcgca gcccaagcga
     2461 tcaactggat cggcaccgag gtgcccacca cgccgcgggc cgcgatcgtc ttcgacatcg
     2521 acgagaccgc gctgtcgaat tgggaggtga tccgggccaa cgactttggc cgcttcatca
     2581 acggaccgtg caacctgccc gacgcgtgcg cctggcgggc gtgggacgag accgcgcagt
     2641 ccacggtcat cgcgccgaca ctggacacct acaacaccgc gaagagcctc ggcgcggccg
     2701 tcttcttcat cactgggcgc gacgaaggcc agcgcgccgc caccgagcag aacctgcagt
     2761 cggccggtta caccggctac acccggctga tcatgcctgc gcccggcgcc caatacgcgt
     2821 cggccgcgga tttcaaggcg ccacagcgca ccgcaatcga agacgagggg tacacaatcg
     2881 tcgcgaacat cggtgaccag ccgtcggacc tggccggcgg ccactcgcag cagacctttt
     2941 tgctgcccga accgttctac cgcatcccgt gaacggccga gacggtaact agcgcggaaa
     3001 agttcgagtg gggaccacgt ggaataccgt ttcggcgcga tgcaggcgtt gacttgtgcg
     3061 cgattaggaa ttaggcgtga aatagaacca tgagcgctcc agaagaaaag agatgcacct
     3121 gccactgcgc gaggtgcgcg gccggtaacc actgcggcaa ctacggcagc ggctgccaga
     3181 agttctaccg ccgccgccgc taaccgattg agagcgcgat gccgtcgagg atgtcgtgct
     3241 cgctgacaat cagctggtcg atgcccgcgc gctgccgtag cgcataggcc agttcttcga
     3301 ccacgatcgc cccgccggcg atcacgtcga cccggccctc gtgcatggga cccagtacgg
     3361 cgcgctgcgc gtgcgtcatc gcgatcagcc cctcgcacac gccgagcaac tcgggaaaac
     3421 ccacccgcga caggtggatt gcctccgagt cgtagcgagt catcttctgc gccaacgccg
     3481 acagcgtggt catcgtgccg gccaccccga cccacgcgtg cgcgcggtcc accggaacca
     3541 ctcgcagcgc ctcgcccagc cgctcacgga cgacctcccg ggcggcggcc acctcggtgg
     3601 cggtcggtgg gtcggagtgc agacagcgct cggtgagccg cacgcagccg acgtccgccg
     3661 agaagctggc atccacatcg tcggtgccca gcaccagttc cgtggagccg ccgccgagat
     3721 cgacaaccag gaaaggcgct tcagcgcaat ccaattcggc gaccgcaccg tgaaaggaca
     3781 gcgccgcctc ctcctcgccg gtgatcactt cggcgacggc ccccggtacc gcggccgcca
     3841 aaacctcggc cgtcatcgcg aagaactcct gccgattcgc tgcgtcgcgc gccgccgacg
     3901 tcgccaccat ccgcaccttc ggcacctgca agcgggcaag caattccgtg tagtcggtca
     3961 acgcggcgcg ggtacgggcg atcgcctccg gcgcgaattc gcctgtggcg tcgacgcctt
     4021 ggccgagccg cacgatgcgc atctcgcggt gcagttcgtg cagcccgccg tcggcgacct
     4081 cggcgatcaa caaccggatc gagttggtgc cgcagtcgat cgcgccgact ctcacgtcca
     4141 atcctctttg accaggaggc cgtccatcgt cgggtcggtc gccaacagcg ccagagcctc
     4201 gtcacccagc gggttgacgc cgcgaccctt ggcaagcgag tgcgcgatca gcacatgcag
     4261 gcacttcacc cggtcgggca tgcctccacc ggagaacgta gtgcccaacg gttcgacggc
     4321 gtcgcgctcg gccagatagc tctcgtgggc acgccgatac gcggcggcca gctccggctc
     4381 ggcggaaagc cgcgtcgtca tctccttcat caaccccgag gattccagcc ggctggccgc
     4441 cgcggtcaac gcggggtggg tcaggtagta cagcgtcgga aacggcgtcc cgtccggcaa
     4501 ccgcggcgcc gtcttgacca cgcccggctc gccgttgggg catcggtacg cgatcgccag
     4561 cacgccgcgc ggttccctgc ccagttgacg tgcgaccgcg tcgaggtcgg cggggtcaac
     4621 caccgggggg aggcgggggt ggtcccggcg gaggtggcgg tgcgggcggc ggcgcggcgg
     4681 gcgaggggat gttgtgggga gtgtcggcga tcgtgcgcca aagtgcggtg taccacgggt
     4741 cattcgagga tgtcgtcgat gaggacgcgc ccggctgatc cgacggtgtc accgcccccg
     4801 gcggcagttg cacctgatac gggacgtcgc cgggcatgac gaacccgagc cgctcgcggg
     4861 cctgtgcggc gatgaatacg ggatcgccga gcttggcctt ctgttgctcg agttcggcga
     4921 tctgcgtacg caactgagct tcgctggccg ccagctgctt catctccgtg cgctgtgcga
     4981 aataggttct taccgggccg gcgatggtca gtgtcaatac acagatgacc gcggccagaa
     5041 tcgccgctcg ccgcgccgtg aacccgagcc gctgctcgct ttggcgcgcg gccgccgcgg
     5101 cgatttgcgc ggtgatcgac tcggtgacgg gctcttcggc cggtcgttgt tcggctgcgc
     5161 gaggctcccg gcgcggcgcg gtcgaccgcg ggcgttcccg aaccgattcg ctgccccggc
     5221 tgggacgtga acctggggag cgtcgtttcg gatcaggccg cttcgcgtcg ggcaccggtt
     5281 agtcggcctc cagcgcaaac cgcggaaagg caaggtcacc ggaatagcgc gcggcgtcgc
     5341 cgagcgtctc ctcgatccgc agcaactggt tgtacttggc gacgcgctcg ctgcgcgccg
     5401 gcgcgccggt cttgatctgg ccgctgccca ccgcgacggc caggtctgcg atcgtggtgt
     5461 cctcggtctc cccgctgcga tggctcatca tcgtgcggta gccgctgtag tgcgcgagcg
     5521 cgaccgcgtc cagcgtctcg gtgagcgtgc cgatctggtt caccttcacc aatagcgcgt
     5581 tccctgcgcc cttttcgatg ccctcctcga gacgttccgg gttggtgacg aacagatcgt
     5641 cgccgaccag ttggacgcgg tccccgatct ccttcgtcag cgcgacccag ccgtcccagt
     5701 cgtcttcgga cagcggatcc tcaatcgaca caagcggata cgtgtcgagc aggccggcat
     5761 agaacgcggt catctgcgcc gagtcgcggg tttccttctc gaacgtgtag ccggtgtcgg
     5821 tgctgaagaa ctcggtcgcc gcgacgtcga gcgccaacgc gacctcggaa ccgggcttgt
     5881 acccggcaga ttcgatcgca gacaagatca ggtcgagcgc ggccttggtg cccgcgacgt
     5941 cgggggcgaa tccgccttcg tcgccgaggc ccgtcgagag tccctgtttc ttcagcacgg
     6001 acttgagcgc gtgatacacc tcggcgcccc agcgcagtgc ctccttgaaa ctcggcgcgc
     6061 cgatcggggc gatcatgaat tcctggacgt ccacgccggt gtcggcgtgc gcgccaccgt
     6121 tgaggatgtt gagcatcggg accggcagga tgtgcgcgtt cgggccgccg atgtagcgga
     6181 acagcggcag cgcggccgaa tcagcggccg ccttggccac cgccagcgac accccgagga
     6241 tcgcgttggc gcccagccgg gacttgtccg gggtgccgtc cagatccagc agcgcctggt
     6301 ccaccagccg ctgctcgttg gcggacatcc cgatgacggc cggcgcgatc tcgtcgagca
     6361 ccgcgttcac cgccttctcg acgcccttgc cgccgtagcg ggacgccccg tcgcgcagct
     6421 cgacggcctc gtgttcgccg gtggacgctc ccgacggaac cgcagcccgc gcgaacgtcc
     6481 cgtcggtcag ggccagctcg acctcgacgg tggggttgcc gcgggagtcg aggatttctc
     6541 gggccccgac ctgctcgatg atgggcactg cgtctccttc cggcgggctg aacgcactca
     6601 ccctatcggt gtgtcctcac agcgggcggc cgtcggcgta agcggtcgcc cagtcgcgca
     6661 cggttctggc gtactggtcg gacaggttgt aggcgcgtag cgctttcatc catccctccg
     6721 gcgtggccag gtcgccaccg cgccagcaca ggtacccggc cgccgacagc gccgcgtcgt
     6781 cgaagttgtc cgggctgacg acgccgtcgt tgttggcgtc gacgccgtag agccgccagg
     6841 tttcggggat gaattgcatc ggccccatcg cgcggtcgat tccgcgatcc ccgtcgagca
     6901 ggccgccgtc gctgtccatg atctcgacgt tgccgtggga gccgtcgagc tggacgccgc
     6961 ggatcagcgg ggtgacgtcg ccgttggggg cgatggcggc gccgcgatag gtgccattgt
     7021 ggctctcgac catcccgatc ccggccagcg tggtccacgc gaggtggcag tccgggtttt
     7081 ccacttcggc cacccgcgcc gcgtaggcgt aggcctccag cgcgtttacc ggcatgttga
     7141 gcgcgggcgc gcgggcatcg gcccaggcgt gcagccggcc ggccggccgg ccggcggcgt
     7201 gggtgtcgat ctcgggcacc ggatcgcccg ccggtggcgg cactccctcg gggatcggcg
     7261 tgccgaggtg ccacgagcag ctggacgcca tcaacaccgc agtgaccgcc gcgacggcga
     7321 cggcccgcac ccaacgcacc cttgtcaggc ccgctgcgcc gtccctcgcg cgagggtgag
     7381 accccctgtc ctccggcgac accgcatctc ctcaacccac tctctggtcc gtcccatcgt
     7441 cccatgcgat cgacggccgt cggcccccct tcggcacggt atcggaccca taccgagaaa
     7501 ttctcaggct tgctaaggtg agccacacat aactttgggg agccaaagct gacctaagga
     7561 cagcgcatga ctgcaggtgc cgacttcacg agcgtgacag ccgccggtcc cacgctgtcc
     7621 tcccaattgc tgggcagcgg actgatcgga ctgcgcgagg gcctggaagc cgggatcgtc
     7681 gtgatgatcc tcgtcgcgtt cctggtgaag gcgcaacggc gcgacgcact gaagtgggtg
     7741 tggctcggcg ttgccgcggc ggtggcgatg accattgcag tgttcttgtt catccagtac
     7801 agcgcctaca cggtcaccga tctcggtgcc gaggcgatcg ccggtatcgc gtcgctgatc
     7861 gcggtcgcga tcgtcaccac gatggtgttg tggatgaaaa aggccgcggc cggaatgtcc
     7921 ggcgagttgc gcgccgagat ggccaaggcg ttggagacag gctcgctggc ggtgctgacg
     7981 ctggcgttcc ttgcggtggg tcgcgaggga ttcgaaaccg cgctgttcat ggtcgggtat
     8041 gcggaggccg aaaccgtctg gccgctggtc ggattgttga tcggcgtgct gctggcagcg
     8101 gggatcgcct gggggatgta catgggcgcg gtccggatca atctggccaa attcttcacc
     8161 tacaccggca tcttcctgat cttcgtcgcc gcaggcattc tcggctacgg cgtgcacgcg
     8221 ctgcaaaccg tcggctggct tcccggtctg ggcgccaagg cttttgacat cacgggctgg
     8281 atgaactggt cggcgtggta cggcgagttg atccagggca tcttcaacat caccccgacg
     8341 ccgacggtat tgcagctgat cgtctacctc acctacctcg tcgtggtgct ggcggtgttc
     8401 ttacgtccca accgcgcgcc tgcgcgtccc actggcggtg ccgaatcgtc ggcctccgca
     8461 gacggtgccg caccgtcggc accgtctgcc tctcaaccaa cacccgaaag gtccactacg
     8521 tgaacgtccc ccttccccgc tcgctagtgg cggccgtggc tgccgtgctg gccggtgttt
     8581 cgctggtgag ctgcgccgcc aaggagtcgg acaacaactc gtccgcgtcg ggcgccgcga
     8641 cggaggtcac cgtcaacgcg acggacactg cctgtgagct gtcgaagacc gaggccaaaa
     8701 ccggcaccac gaatttcgtg atcaccaaca acgggaccaa ggtcaccgag ttctatgtct
     8761 acggcgaagg cgagcgggtg atgggcgagg tggagaacat ctcccccggg ctgcagcgca
     8821 agctcgtcgt aaccctcaac gagcccggca cgttccagac cgcgtgcaag cccggcatga
     8881 tcggcgacgg tatccgcggt gacttcaccg tgaccggtga cgccgtcacg accaacgccg
     8941 aaggcaagtt ccaggtggcc tccgagaact acaagcagta cgtcatcggc cagaccgacg
     9001 cgctggtcac tgccgtcgaa gcgttcgtcg ccgcgatcaa ggctcgcgac atcgcgtcgg
     9061 ccaagacgca atacccgacc agccgtgtct attacgaacg catcgagccg gtggccgagt
     9121 cgttccccga cgacctcgat ccccgcatcg acttgcgcga ggccgacctc gaaccgggcc
     9181 agaaatggac cggcttccac cggctggaaa aggacctgtg ggtgacgggc ttgcagcccg
     9241 acaccaatgc gatcgccgac cagctcgtcg ccgacgtgcg tgaactgaac cagaaggtga
     9301 agtcaccgga ctacacgatc gactccacgc agatcgccgg cggcgcacag ggtttgctcg
     9361 acgagatcgc gatcagcaag atcaccggcg aagaggacat cttcagccac accgacctgt
     9421 gggacttcaa cgccaacgtg gaagggtcgc aggccgcggt ggggtcagtt cgacccattc
     9481 tcgacgagcg caacgccgaa ctcggcaagc gcgtcgacca gcggttcgcc gaggtgttcg
     9541 cgctgctgga caggtatcgt cagggcgacg ggttcgtctt gtacgacacc gtgaccgagc
     9601 cgcagcgcca agaactttcg cgtgcgatcg acgcgctgtc caaggaagta agccaggttc
     9661 aaggtgtcat cgccccccaa tagcgagcag ctagaggacg attcccccac gccagagcgc
     9721 ggggggatct cccggcgaaa actgttcggc gcggccggag tcaccgcggc ggtggtgggt
     9781 gcggcaggtg ccggcgcact cgccggccgc gcgtcggcgg cggcggccga cgactccacc
     9841 gaaatccacc ggcctatccg gttccgcggc gaacggcagg ccggcatcgt caccccacag
     9901 caagaccgaa tgcacttctg cgcgttcgac atcacgaccg acaagcgtga cgacgtcgta
     9961 gcgatgctgc gggagtggac gcggatggcc gagcggatgg cggccggcca cgaaaccgtc
    10021 cccgacggcg cggtggggct caacccgtat gcgcccccgg cggacaccgg tgaggcgctt
    10081 ggccttcccg cgtcggcgct gacgttgacg atcgggttcg gtccgtcgtt cttcctcaag
    10141 gacggcaagg acaggttcgg catcgccgac cgcaagccgg cggcgctggt ggatctgccg
    10201 aagttcccga acgagaccat ggatccggcc cggtgtggcg gggacatctg cgtgcaggca
    10261 tgcgccaacg acccgcaggt ggcggtgcat gcgatccgca acctggcccg ggtcgcgttc
    10321 ggcacggccg cggtgcgcta ctcgcagctg gggttcggcc gcacctcgtc caccacgcgc
    10381 gaccagtcga caccgcggaa cctgttcggc ttcaaggacg gaacgcgcaa catcaaggct
    10441 gaggacaccg acaagctgca tcagcacgtc tgggtggcag caggtgacgg cccggactgg
    10501 ctgaccggcg gcacgtatct ggtcgcccgc cgcatccgga tgcgcatcga gaactgggac
    10561 cgcacagcgc ttttggagca ggagcgggtg atcggccgac acaagggcag cggtgcgccg
    10621 aacggctacc ccgacgagtt cgacgaactc gatctggaca gcaagaacgg cgacggcgac
    10681 cccctgatcg acgtcgacgc gcacgtgcgg ctggcctcag ccgagcagct gggcggcatc
    10741 gaaatcttgc gccgcggcta caacttcacc gatgggtcgg acggcttcgg ccacttggac
    10801 gctggcctgt ttttccttgc gttcgtgcgc aatccgctga cccagttcgt gccgatgcag
    10861 gctcaactcg ccagcagcga cgcgctcaac gagtacatca cgcacaccgg cagtgcggtg
    10921 ttcgcgtgcc cgcccgggat gcgggagaac gactcgtcgg cttattgggg ctcggggctg
    10981 ttcggctgag cgttcgcgac cggtcgggga gtcaacacag ggcacatccg cggccaggga
    11041 ccagggggtg ttgtctcacg cttcgtccgc tccagcgccc tgattgatct gctggttcag
    11101 ctcggggaac cacggccgca cccactgacc acggtggcgt ttgctgagca gaaatgtgca
    11161 cgagcgggca atcccgggtg gtttgcagcg ccatcaccat cctccaaaag ttgagaagtt
    11221 ggtcagcgtc ctcactgccg ctggtcagat gtagtccggt ggctgcgaaa gcaccggcac
    11281 taattcgtgc aagggtttcc ggcgtagtag ccacatcacc aaggaacggg tgcggcagct
    11341 ccaccatcag caacagtcgc agcggcgcgg ccatggagaa ggcgtcgtag cgtcggtcga
    11401 cgtggcagtc ctcggcccat tcgtagagcg atagtgcttt gtggaagccg atgccgaggc
    11461 ttctttgcgg tggtcgtgta cgggcgacga acctgagttg cccttagccg tcaggtgttt
    11521 cgtccgccgt tgacgccgat gatctggccg gtgatgtagc cggcttcatc ggagacgagg
    11581 aatgcgcagg ttgcggcgat atcttcgggg cgtccgatac gtcgtacggg ggtggcggcg
    11641 acgaggtcgt cgagggtgtt gcggccgaag tcaccacgct gggctgcgtt gcgcagcatc
    11701 ggggtgtcga cgaagccggg gggcacggcg ttgacggtga tgcctttggg gccgtattcg
    11761 agtgccaggg atttggtgag accgttgacg ccggatttgg cggcgacgta gtgggttttc
    11821 atgggtgtgc cggagtgggt gctcgaagag gagatgttga cgatgcgtcc ccagccggcg
    11881 tcgagcatgt cgggcagcac tgcctgggtt gtgtggaaca cgccgttgag ggtgacgtcg
    11941 atgacgcgct gccattcggc gaaggagatg tcgaggaacc gcgcggagcc ctcggcgcct
    12001 gcggcgttga ccagaatggt gaccgggccg aatcgtgacc ggacgtcggc cagtgcgttg
    12061 tcgatttgcg cgcggtcggt cacgtcgacg gtgtatgact gaccgccttc ggcgggggtg
    12121 atgtcgagag tggccacctg gtagccatcg gtgatgaggc ggtgcacgat cgcggcgccg
    12181 attccggagg caccgccggt gacgatggcg gtgcgtgggt tgctggcggg tggcatgggg
    12241 gctccgtggg aatgttcttg tggttcagcg atgtggtggg ggttcaggca gtcccaatgt
    12301 tcgacgaccg acctcgatca ggtgggcacg cagggtgtcg tcgtcgattc ggtgtggtgc
    12361 ggtcgaggcc ccgccggcca gcccggtcat gacgacagcg gcgttgatcg accctgacac
    12421 agacgaatcg gcgccggcca gcagggcgag ctggcggtcg atgaccgccg accactcggg
    12481 ttggttgcgc agcacgcagc ggacactggg gtcgaaggtg gtcacggcgg ccagggttcg
    12541 gtttctggag acgatgtcgg cgtagccggt gaccataacc tcggcgcggg cgcgggcgct
    12601 gcgttgacgc tcg
//
LOCUS       DASWBF010000528         3240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_973518,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000528 DASWBF010000000
VERSION     DASWBF010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973518"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VF874_19725"
     CDS             complement(<1..421)
                     /locus_tag="VF874_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9178205.1"
                     /translation="MRHLMFEEAGRYAWRDAPEPTVSGPEQAIVRPVAIACCDLDVGV
                     AQGVLPMPPGHAVGHEGIAEVVAVGDAVTGVRVGDRVVVPFQISCGQCRECRRGVTGS
                     CGSAPLMAMYGMAPLAGLDGGGFMADLVLVPYADAMLI"
     gene            528..1109
                     /locus_tag="VF874_19730"
     CDS             528..1109
                     /locus_tag="VF874_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9178206.1"
                     /translation="MPRPDRSRAALIDTAGTLFRRQGYAATGLNQILDDAGVKAGSLY
                     HHFPGGKQELAAAVVESAGAQIERVLRAALADDASVASVVDRWLDMLAAGLAADCRDG
                     CPVEPIATESVHASDLVRRAAACAFDSWCAAIAERLRKDGWDSTRADQTALAVVSIVE
                     GALVLSRTSGDVAALRAAKNAAQSLLRSEIRDN"
     gene            1113..1376
                     /locus_tag="VF874_19735"
     CDS             1113..1376
                     /locus_tag="VF874_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178207.1"
                     /translation="MTATSRSESKWSLWSEGSVLSIGSKGSVLSIGSIGSALSIGSVG
                     SILSVGSIGSAGSLLSALSARSIGSLMSYRSAWAALSAPRSKD"
     gene            complement(1377..2234)
                     /gene="nadC"
                     /locus_tag="VF874_19740"
     CDS             complement(1377..2234)
                     /gene="nadC"
                     /locus_tag="VF874_19740"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX9178208.1"
                     /translation="MRLGEHEVAEARAVIRRALDEDLRYGPDVTTEATVPADATTTAA
                     LVAREGGVIAGVDVALLVLDEVLGIGGYRVLQRLSDGDRLERYVIALRVEGDTRGLLT
                     AERTMLNLVCHLSGIATATRQWVDAVAGTRAIVRDTRKTLPGLRALQKYAVRVGGGQN
                     HRMGLGDAALIKDNHVAAAGSVVAALRAVKAAAPGLPCEVEVDSLDQLDDVLAEQVEL
                     VLLDNFPVWQTQIAVQRRDARAPKVKLESSGGLSLDNAHAYASTGVDYLAVGALTHSV
                     RVLDIGLDL"
     gene            complement(2234..>3240)
                     /locus_tag="VF874_19745"
     CDS             complement(2234..>3240)
                     /locus_tag="VF874_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9178209.1"
                     /translation="ATGGLGNLYRATTNPEGSTGDGVALALWAGVPVSDIEFVQFHPT
                     MLCDGRVEGRRPLITEALRGEGAKLVDAQDVSVTADVHPMGDLAPRDVVAAAIEARLR
                     QTGDPCVYLDARAVSDVARRFPTVTAACRAAGIDPAHDLIPVVPGAHYSCGGVVTDVH
                     GRTELAGLFAAGEVARTGMHGANRLASNSLLEGLVVGGRAGRAAAEHTTARGAVVASS
                     PEPSTRKALGRKDLQRLMSAHASVVRDAHGLTELEQRLEVAPACAARSRSAIEDIALT
                     VTARAVAAAALARSETRGCHHRADCPHTDPAQAQSISVRLASDGGVVVNAPVPAGACG
                     "
BASE COUNT          488 a         1155 c         1088 g          509 t
ORIGIN      
        1 ggatcagcat cgcgtccgcg tacggcacca gcaccaggtc ggccatgaag ccgccgccgt
       61 ccaggccggc cagcggggcc atcccgtaca tcgccatcag cggcgccgag ccgcacgatc
      121 ccgtcacgcc ccgacggcat tcgcggcact ggccgcagct gatctggaac gggacgacga
      181 cacggtcacc gacccggacg ccggttacag cgtcacccac tgcgacgacc tcggcgattc
      241 cctcgtggcc caccgcgtgc cccggcggca tcggcaagac cccttgagcc acgcccacgt
      301 cgaggtcaca gcaggcgatc gccactggtc gcacgatcgc ctgctccggc ccggaaaccg
      361 tgggttccgg ggcgtctcgc caggcgtatc gcccggcctc ttcgaacatc aagtgccgca
      421 ctcttgcctt ctctcttcgc gcaagcggct catcggaagc cttctctctt cgcgcaagcg
      481 gctcatcgga agaattgttg aatgatcttt ctagtattgt caagggtgtg ccccgccctg
      541 accgcagccg cgccgcgctg atcgacaccg caggcaccct gttccgccga cagggttacg
      601 ccgcgaccgg cctgaaccag atcctcgacg acgccggggt gaaggccggc tcgctgtatc
      661 accacttccc cggcggtaag caggaactgg ccgcggccgt ggtggagtcc gccggcgcgc
      721 agatcgagcg agtgttgcgg gccgcgcttg ccgacgacgc ttcggtggct tcggtcgtcg
      781 accgctggct ggacatgctc gcggccgggc tggccgcgga ctgccgcgac ggctgcccgg
      841 tggagccgat cgccaccgaa tccgtgcatg cgagcgacct cgtgcggcgg gccgcggcat
      901 gcgcgttcga cagctggtgt gcggccatcg cggagcggct tcgcaaggat ggctgggata
      961 gcacgcgcgc agatcagaca gcgctcgccg tcgtttcgat cgttgaaggg gcgcttgtgc
     1021 tttcgcgcac gagcggcgac gtcgcggcgt tgcgcgccgc aaagaatgcc gcgcagtcgc
     1081 tgttgcgtag cgagatccgc gacaattgag acatgactgc tacttcgcgt tcagagtcca
     1141 agtggtcgtt gtggtcggag gggtctgtgc tgagcatcgg cagtaaagga tcggtgttgt
     1201 cgatcggctc gatcggctcg gcgctgtcga tcggctcagt cggatcgata ttgtcagtcg
     1261 gctcgatcgg ttcggccggt tcgctgctct ccgcgctgtc ggcccggtca atcggctcgc
     1321 tgatgtccta ccgctcggcg tgggccgcgc taagcgcacc acgttcgaag gactgactac
     1381 agatccagac cgatgtcgag cacccgcacc gagtgcgtga gtgcgccgac cgcgaggtag
     1441 tcgacgccgg tcgacgcgta cgcgtgcgcg ttgtcgagcg acagcccgcc ggacgattcc
     1501 agcttcacct tcggtgcacg tgcgtcgcgg cgctgcacgg caatctgcgt ctgccacacc
     1561 gggaagttgt ccaggaggac cagctcgacc tgctcggcaa gcacgtcgtc gagctggtcg
     1621 agagagtcga cctccacttc acacggcagc ccgggtgcgg cggccttgac ggcgcgaagc
     1681 gccgcgacca ccgacccggc cgcagcgacg tggttgtcct tgatcagcgc cgcgtcaccg
     1741 agtcccatcc ggtggttctg tccgcccccg acccgcaccg cgtacttctg caacgctcgc
     1801 aggccaggca gcgtcttgcg cgtgtcacgc acgatcgcgc gggtgcccgc taccgcgtcc
     1861 acccactgcc gggtcgcggt ggcgatgccg gacaggtggc acacgaggtt gagcatcgtc
     1921 cgctcggccg tcagcaatcc ccgcgtgtcg ccctcgaccc gcagcgcgat gacgtaacgc
     1981 tcgagccgat ccccatcgga caggcgctgg agcactcgat agccacctat gccgaggact
     2041 tcgtccagca ccagtagcgc aacatcgacc ccggcgatga ccccgccctc cctggcgacc
     2101 agcgcggccg tcgtcgtcgc gtcggcgggc accgtcgcct ctgtggtgac atcaggcccg
     2161 tatcgcaggt cctcgtcgag cgcacgacgg atcaccgcac gggcctcggc gacctcgtgc
     2221 tcgccgagcc gcatcagccg caggccccgg cgggcacggg cgcgttgacg acgacgccgc
     2281 cgtccgatgc cagccgcact gaaatgcttt gtgcctgtgc gggatccgtg tgcggacagt
     2341 cggcgcgatg gtggcacccg cgggtctccg accgcgccag cgcagcagcc gcaacagcgc
     2401 gcgcggtcac cgtcaacgcg atgtcctcga tcgcggagcg gctgcgggcg gcgcatgcgg
     2461 gcgcgacctc cagccgctgc tcaagctcgg tcagcccgtg ggcgtcacgg accaccgacg
     2521 catgcgcgct cataagccgc tggagatcct tgcggcccaa tgccttacga gtcgaaggtt
     2581 ccggcgacga cgcgacaact gcgccccgag ccgtggtgtg ctccgcggcc gcgcgtcctg
     2641 cccggccgcc gacgaccaga ccttcgagca ggctgttgga agccagccgg ttggccccgt
     2701 gcatacccgt tcgcgccacc tcgcccgccg cgaacagccc cgcaagctcg gtgcggccgt
     2761 ggacgtcggt gaccacgccg ccgcagctgt agtgcgcgcc tggcacgacg ggaatcaggt
     2821 cgtgcgccgg gtcgataccc gccgcgcgac atgccgcggt gacggtggga aacctgcgtg
     2881 caacgtcgga gaccgcgcgg gcgtcgaggt agacgcacgg gtcgccggtc tgccgcagcc
     2941 gcgcctcgat ggcagcagcg accacgtcgc ggggggcgag gtcgcccatc gggtgcacgt
     3001 cggcggtcac tgaaacgtct tgcgcatcaa ccagtttggc gccttcaccg cgtagcgctt
     3061 cggtgatgag cggccggcgg ccctcgaccc ggccgtcgca aagcatcgtc ggatggaact
     3121 ggacgaactc gatgtcgctc accggcacgc ccgcccacag ggcgagcgcg acgccgtcgc
     3181 ctgtcgaccc ttccgggttg gtggtggcac ggtacaggtt gccgagtccg ccagtcgcga
//
LOCUS       DASWBF010000529         6747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_571223,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000529 DASWBF010000000
VERSION     DASWBF010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571223"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VF874_19750"
     CDS             <1..758
                     /locus_tag="VF874_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178210.1"
                     /translation="TLKSRGAAGVWDDSQEFVAEYLRAVGLSTRPDWVYGVGPRGVWS
                     DDPGRWFIAHAATPDMLASARAHAPGAGPPPAPSPAIAFVSVAQFPVALVRRAKGEPA
                     PSIADAIVGAIVYGGLAPGDCFGACPLGIPQYVHEENSCAGMTVGSNGVVITAAYDRI
                     APWHEAFRRDAPHPVPFDGPIVGRSGPVTVVRLDYGDVFARRVEKGDAMVPASPIELA
                     VAFVETVGASSLPTAGASARCSWGSTQGTRMRR"
     gene            755..919
                     /locus_tag="VF874_19755"
     CDS             755..919
                     /locus_tag="VF874_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178211.1"
                     /translation="MIYRPGPDDAAGRDRFSRYIVALGADPAALDYGRREGRMMASGK
                     RFKMVIGSGV"
     gene            947..1093
                     /locus_tag="VF874_19760"
     CDS             947..1093
                     /locus_tag="VF874_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX9178212.1"
                     /translation="MKTHLNCPCGEAITGTDEDDLVEKTQAHLADAHPGLQYDRDAIL
                     FMAY"
     gene            complement(1111..2193)
                     /locus_tag="VF874_19765"
     CDS             complement(1111..2193)
                     /locus_tag="VF874_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178213.1"
                     /translation="MPAAKKGHRTLFAAVAALAVAGSWATAGVAAADPAPPAIPIDIA
                     QAPGLPAMEQLSPVLQQAAANPQNAASMLMAAASAFAGNSAAPADSRQVATSVNQFVQ
                     QPSGPSGPPAQGIQIKLPNKFAMPGNLNQLLPAAATAPAPANPAAPASPVVHVPAVGA
                     EPGTVPHLPTGVDPANAVGPATGAALAPAPAAAPDSTPPPAAAPDPAPPPPAPGPAPA
                     PSAFGPDAPPTQDFMYPSIGNGCLADGGNSIATAMSVAGPASIPKPGPKAGQTAYVFT
                     AIGTPGPAAEQKLPLNVTWVNLTTGKSGSATLKSNPEINPDGPTTLVAIADTGSGSVM
                     STIFGQVTTVEKQCTFLPTIGSTVVP"
     gene            2287..3555
                     /locus_tag="VF874_19770"
     CDS             2287..3555
                     /locus_tag="VF874_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase"
                     /protein_id="HEX9178214.1"
                     /translation="MRRALIAKVGRIDTVTTAPSPTRDRLATIAPLLLALSVAARLAW
                     TYLAPNGANFVDLHVYVGGAATIGTDHALYDFVYADQTPDFPLPFTYPPFAAVVFYPL
                     HLLPFGFVSFAWTVGIMAALYGVVRVSQRLLGRGSHRAAMCWTAVGIWIEPLRSTLDY
                     GQINVLLVLAVLYAVYSTRWWLSGLLVGLAAGIKLTPAISGLYFLGVKRWGAALFAAA
                     VFVGTVGVSVLVLGDQARYYFTDLIFNAERVGSVATSQNQSWRGGISRIVGHDAGYSV
                     PVIVAIAVTAVLAVLAWRAIGAGDAEKPDRLGGIVVVQLFGLLLSPISWTHHWVWLLP
                     TMIWLIHGPLRDRLGARILGWGWLALTLIGPPWLLSFAQPTIWQTGRPWYLAWAGLVY
                     IVAAVATMAWIAATGRRSRRSVSPAQDPSA"
     gene            complement(3500..3784)
                     /locus_tag="VF874_19775"
     CDS             complement(3500..3784)
                     /locus_tag="VF874_19775"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX9178215.1"
                     /translation="MAVLSDEEVDSALPELAAWERVDKALRRSVKFPSFLAGIDAVRR
                     IAEAAEAADHHPDIDIRWRTVTFVLSTHSEGGITQKDLALAKQIDEIVGQ"
     gene            complement(3799..4258)
                     /locus_tag="VF874_19780"
                     /pseudo
     CDS             complement(3799..4258)
                     /locus_tag="VF874_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
     gene            4336..4740
                     /locus_tag="VF874_19785"
     CDS             4336..4740
                     /locus_tag="VF874_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9178216.1"
                     /translation="MQNQIVVAGALIAGDRLLVAQRARPPELAGRWELPGGKVAPGET
                     EAAALARELAEELGIEVSVGPRLAGDVALSDTTTLRAYLVTQTCATTASAHDHRAVRW
                     VAVAELAELDWVPADRIWIPELTRLLSADSVR"
     gene            4786..5064
                     /locus_tag="VF874_19790"
     CDS             4786..5064
                     /locus_tag="VF874_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178217.1"
                     /translation="MLAFDRIGSRIPQLIQIWLVELFFVMPLAFFIGKVIDIRGAFGV
                     PGTGESLDRVFWGRAGGGSGRGVLLRPVARETPCGARQLDTHVACAGR"
     gene            5258..5986
                     /locus_tag="VF874_19795"
     CDS             5258..5986
                     /locus_tag="VF874_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9178218.1"
                     /translation="MALARVLAWYVFRFGRNKLDAQLGGLPISQRRLGWEWWDEKRTI
                     AALPVVTVADSQSAAGEYRRVEGTLDNLVTDGRVERVEPDHRRPGRPPLVFRAVRRMD
                     PGGPRRYQMLAEILTLSLAGDRNAGVKALEAGRAWGRGLGSATEKAPETTKAVDQLVD
                     LLDELGFAPERRVAHGEPQVGLRHCPFLEFAEHRSDVVCPIHRGIMQGALETWDAPVM
                     VDRLDPFVEPDLCLARLTPEGTAT"
     gene            5983..6410
                     /locus_tag="VF874_19800"
                     /pseudo
     CDS             5983..6410
                     /locus_tag="VF874_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          955 a         2184 c         2387 g         1221 t
ORIGIN      
        1 ccaccctcaa gtcgcgcggg gcggcggggg tgtgggatga cagccaggag ttcgtcgcgg
       61 aatatctgcg ggcggtgggg ctgagcaccc gacccgactg ggtctacggc gtcggaccgc
      121 gtggcgtgtg gagcgacgat ccgggacggt ggttcatcgc gcatgccgcg acacccgaca
      181 tgctggcctc cgcccgcgcc catgcacccg gcgccggccc accaccggca ccgtcaccgg
      241 cgatcgcgtt cgtctccgtc gcgcagttcc cggttgcgct tgtgcgccga gcgaaaggtg
      301 aacccgcacc gtccatcgct gacgcgatcg tgggcgccat cgtctacggc ggcctggcgc
      361 ccggcgattg cttcggcgcg tgcccgctcg ggattccgca gtacgtacac gaggagaatt
      421 cgtgtgcggg catgaccgtt ggcagcaacg gtgtggtgat caccgcggca tatgaccgaa
      481 ttgcgccgtg gcacgaagca tttcgccgcg atgccccgca ccctgtcccg ttcgacggcc
      541 cgatcgtcgg gcgatcaggt cccgtgaccg tggtgcggct tgactacggc gacgtgttcg
      601 ctcgaagggt tgagaagggt gatgcgatgg tgcccgcttc accgatcgag ctggcggtgg
      661 cgttcgtcga gacggtcggc gcatcaagcc tgccgactgc tggggcatct gcgaggtgca
      721 gctggggatc aacgcaaggg acacgtatgc gcaggtgatc taccgtccag ggcccgatga
      781 cgccgcgggc cgcgaccgct tcagccgtta catcgtcgcc ctgggtgccg atcccgcggc
      841 gctcgactac ggccgccgcg agggccggat gatggcctcg ggcaagcgtt tcaagatggt
      901 catcggtagt ggcgtatgaa caccgtcggt gtgccatggt gagaccatga agacacatct
      961 gaactgtccg tgcggtgaag cgatcactgg cacggacgaa gacgacctgg tcgagaagac
     1021 ccaggcgcat ctggccgatg cgcatcccgg cctgcaatac gaccgggacg cgatcctttt
     1081 catggcgtac tgacgcggtt cgcgtcgccg ctacgggacg acggttgagc cgatcgtcgg
     1141 caggaacgtg cactgctttt cgacggtcgt gacctgaccg aagatcgtcg acatgacgct
     1201 gcccgacccg gtgtcggcga tcgccaccag cgtcgtcggg ccgtcgggat tgatctccgg
     1261 gttggacttc agtgtcgcgc tgcccgactt acctgtcgtc aggttcaccc atgtgacgtt
     1321 cagcggaagc ttctgctcgg ccgccggtcc cggcgtgccg atcgcggtga acacataagc
     1381 ggtttggccg gctttcgggc cgggcttcgg aatggacgca gggcccgcca ccgacatcgc
     1441 ggtcgcaatc gaattgccgc cgtcagcgag gcagccgttt ccgatcgacg ggtacatgaa
     1501 gtcctgcgtc ggcggcgcgt ccggcccgaa tgccgacggg gcgggggcgg ggccgggcgc
     1561 cggtggcggt ggtgccgggt cgggcgcggc ggccggcggt ggtgttgagt cgggcgcggc
     1621 agcgggcgcc ggtgcgagcg ctgctccggt tgccggcccg acggcattgg cgggatcgac
     1681 cccggtgggc aaatgcggga cggtcccggg ctccgcaccg accgcgggaa cgtggaccac
     1741 tgggctcgcc ggcgccgccg ggttcgcggg agcgggcgct gtagccgcgg ctgggagcag
     1801 ttggttcagg ttgccgggca tcgcgaactt gttgggcagc ttgatctgga tgccctgagc
     1861 cggcgggccg ctcggaccgg atggctgctg cacgaactgg ttgaccgacg tcgcgacctg
     1921 ccgggagtcg gccggggcgg ccgagttgcc cgcgaacgcc gacgcggccg ccatcaacat
     1981 cgacgcggcg ttctgcggat tggccgcggc ctgctgcagt accgggctga gctgctccat
     2041 cgccggaagg ccgggggctt gggcgatgtc gatcgggatt gccggtggtg ccggatcggc
     2101 cgccgccacg cctgccgtcg cccaggagcc cgccaccgcc agcgcggcga cggcggcgaa
     2161 cagcgttcgg tgtccctttt tggcggcagg catgactgcg aacgtatctc gatacagaag
     2221 tgactcaagt gacacgtgtg gcgtttatgc aaccgttacg gatgtgagcg gcttcggtga
     2281 tttccggtgc gccgcgcgct gatcgctaaa gtcgggcgga tagacaccgt caccaccgcc
     2341 ccgtcgccga cgcgcgaccg gttggccacc atcgccccgc tgctgctcgc gctgagcgtc
     2401 gccgcgaggc tggcctggac ttacctcgcg cccaacggcg cgaacttcgt cgacctgcac
     2461 gtctacgtcg gtggtgccgc cacgatcggt acggaccacg cgctctacga cttcgtctac
     2521 gccgaccaga cccccgactt cccgctgccg ttcacgtatc cgccgtttgc agcggtcgtc
     2581 ttctacccgc tgcacctgct gccgttcggg ttcgtctcgt tcgcgtggac cgtcggcatc
     2641 atggcggcgc tgtacggggt ggtccgcgtc agccagcggc tcctggggcg cgggagccac
     2701 cgcgcggcga tgtgctggac cgctgtcggc atctggatcg agccgctgcg cagcacgctg
     2761 gactacggcc agatcaacgt gctgctggtg ctggcggtgt tgtacgcggt gtacagcacg
     2821 cgctggtggc tctcgggtct gctcgtcggc ctcgccgccg gcatcaagct cacgccggcg
     2881 atcagcgggt tgtacttcct gggggtaaag cgttggggcg cagcgctgtt cgctgccgcc
     2941 gtgttcgtcg gcaccgtggg cgtctcggtg ctggtgttgg gggaccaggc ccgctactac
     3001 ttcaccgatc tcatcttcaa tgccgagcgg gtgggttcgg ttgctacgtc gcagaaccag
     3061 tcgtggcgtg gaggcatcag ccgcatcgtc ggacacgacg ccggctacag cgtgccggtg
     3121 atcgtcgcga tcgcggtgac cgcggtgctg gcggtgctgg cctggcgggc gatcggcgcg
     3181 ggtgacgccg agaagcccga ccgtctcggc gggatcgtcg tggtgcaact gttcgggcta
     3241 ctgctgtcac cgatttcgtg gacgcatcac tgggtgtggc tgttgccgac gatgatctgg
     3301 ctgatccacg gcccgctgcg ggatcggctc ggcgcccgga tcctcggctg gggctggctg
     3361 gcgctgacgc tcattggtcc gccgtggctg ctgagtttcg cgcagccgac gatctggcag
     3421 accggccggc cgtggtatct ggcgtgggcc ggtctggtgt acatcgtcgc cgcggtggcg
     3481 acgatggcgt ggattgccgc tactggccga cgatctcgtc gatctgtttc gccagcgcaa
     3541 gatccttctg cgtgattccg ccttcggagt gcgtcgacag aacgaaagtc actgtgcgcc
     3601 agcggatgtc gatatccgga tggtgatcgg cggcttccgc ggcctcggcg atgcggcgca
     3661 ctgcgtcgat gccggcgagg aacgagggga atttcacgga gcggcgcagc gccttgtcga
     3721 cgcgttccca cgcagcgagt tcgggtagcg cggagtccac ttcttcgtcg gagagcacgg
     3781 ccataagacc aaaggtacct atggtgtcgc tggtcgtggg gcgtcgcgct ccagcgcctt
     3841 cgcgcaaccg gcaaggaccg cgaggaaact ccgcttgagc acccgggagg tgaccgcctc
     3901 catcaccttt ccgcccgcgc gagccggatg ggtataggtg gtcacccagt cgacgtgtgt
     3961 gccgtcgcct gacgggctga acgtcaacgt gccgccttcg tggtcgaagg ccgggaagga
     4021 ggcggacgat ccggtaggag tagctccgcg gcgcgtcgta ggccgtgatc tcctcgcgga
     4081 accacgcgcc caccgcggtc acctcccgca ccgccccctt gcccggctcc gacgaatcct
     4141 tcgcaaagcc ggccttgagg accagtggag gggcggtcag gctggccgga tcggccagcc
     4201 agtcgaagac ccgttccggc gatgcggcga tcgtgcgttc cacgtgcagc ttgaccatga
     4261 gacgagccta gtgccggccg accgtatacc gtcgtgggcg atgagcgctc gcgcgaagag
     4321 cagatagctt gtgccatgca gaaccagatc gtcgtcgccg gcgcgctgat cgccggagac
     4381 agattgctgg tggcacagcg ggcgcggcca ccggagctgg cgggccgctg ggagctgccc
     4441 ggtggcaagg tggcacccgg cgagaccgag gccgccgcgc tggcccgcga gctggccgag
     4501 gaactgggca tcgaggtgtc cgtcgggccg cggttggccg gcgacgtcgc gctgagcgac
     4561 accaccacac tgcgcgcgta ccttgtcacg cagacctgcg ccaccaccgc gtccgcgcat
     4621 gaccaccgcg cggtgcgttg ggttgcagtt gccgaattgg cggaattgga ctgggtgccg
     4681 gcggaccgga tctggattcc ggagctgacg cgtctgctgt cggcggacag cgtccgttga
     4741 gtatgttctg agcctgtgcg cgtcctgcgg ttcctgtggc aacgagtgct ggcgttcgac
     4801 cggatcggct cgcgcatccc gcagctgatc cagatctggc tcgtcgagct gttcttcgtg
     4861 atgccgctgg cgttcttcat cggcaaggtc atcgacatcc gcggcgcctt cggggtgccg
     4921 ggcaccgggg aatcgctcga cagggtcttc tgggggcgcg ctggtggtgg ctctggtcgc
     4981 ggggttcttc ttcgtccggt cgctcgtgaa accccgtgtg gtgcaaggca gttggacacc
     5041 catgtcgcat gtgcgggccg gtgacttcgc ggtgttcgcg gccaacaccc gggccaccgt
     5101 cacctacccg tacctgacca gccatccgtc gtacgcgctg ttgttgttgc tgaccgcgcc
     5161 gatccccgcg gtgatggtcg ccgcgacgac caaccacggc gacagcacat tctactggcg
     5221 ggttaccggc atcatcgggc tgatcatcat cgcctgcatg gcgctggctc gcgtattggc
     5281 ctggtacgtc ttcagattcg ggcgcaacaa gctcgacgca caactcggcg gcctgccgat
     5341 ctcgcagcga cggctgggct gggagtggtg ggacgaaaag cggacgatcg ccgcactgcc
     5401 ggtcgtgacg gtcgccgaca gccaaagcgc ggcaggcgaa taccggcggg tggaaggcac
     5461 gctggacaac ctggtcaccg acggtcgtgt cgagcgcgtc gagcccgatc accggcgccc
     5521 cggccggcca ccgctggttt tccgggcggt tcgccggatg gacccgggtg ggccgcggcg
     5581 ctatcagatg ctcgccgaga tactcactct cagtctggcc ggcgatcgaa acgccggtgt
     5641 caaggcgctc gaagccggcc gcgcgtgggg acgtggcctc ggttcggcca cggaaaaggc
     5701 gccggaaacc acgaaagctg tcgatcagct ggtcgacctg ctcgacgaac tcgggttcgc
     5761 gccggaacgc cgtgttgcac acggtgagcc acaggtaggg ctacggcatt gcccgttcct
     5821 cgagttcgcc gagcacaggt cggacgtggt gtgcccgatt catcgcggaa tcatgcaggg
     5881 agccttggaa acctgggacg cgccagtcat ggtcgaccgc ctggacccgt tcgtcgagcc
     5941 ggacctgtgt ctggcacggc tcacaccgga gggaacggcc acatgagtct cggcccggcg
     6001 atcgcggtcg cgctcatctt cgtctggctt ggcaaggtgc tcgccaattc cttcctcgaa
     6061 gcgccgctga agttccgcgc gcccggggtc accctgcaga tcggactcgg catcggccgt
     6121 ctggtctttc gcgcgctcaa cagcgccgag gtggtcttcg cggtcatcat cgcgattgcg
     6181 ttgacaccga cccacccgcc ggttggtgtc atatcggcgc tggcaccgca ttcgtcgcac
     6241 tggccgcgca gttgctggct gtgcgtccgc gcctgacacg ccgctcgaat gcggtgctga
     6301 cgggtcagga cgcgccccgt cgctctcggg ggcactacgt ctacgtcgcg ctcgaggtga
     6361 tcaaggtgct cgcgttggcc gtcggcggga ttctcttact cgccagctga accgcgatca
     6421 tttccgtcgc gctcgcttgt acaccttctt tgagttgctt gccgtgcacc ccggagcggt
     6481 ctgcctttcg cagtgcaccg atcacgaccg ggcaccgctt gcatcgcggc ttgcggcgac
     6541 ttcacttctg cttcggctgt gcggcgatga gcttgctggg ctctacgttt accttcggcg
     6601 tcaacccgca gccagggcgg cggccagcgt ggcgacgaac cttgggatcg catccgggtc
     6661 gcccgtggat cgggtgggat tcagttcgcc gatcgacagg acgcgtgcgc ctccgcagcg
     6721 cttgacctga tccagtgtga tgcgcag
//
LOCUS       DASWBF010000530         5757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_262180,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000530 DASWBF010000000
VERSION     DASWBF010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262180"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VF874_19805"
     CDS             <1..743
                     /locus_tag="VF874_19805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9178219.1"
                     /translation="RLVNGEIDLAELDPEFSLRRSAEGMAVRTRFFDRMFLDAGTMGV
                     RQAVILAAGLDARAYRLPWPDGTVVYEVDQPEVIEFKTSTLAELGAKPAAVHRPVAID
                     LRNDWRNALLDNGFDPTAPTAWSAEGLLIYLPPQAQDQLFDTITELSARGSRLATEHI
                     PDTNVFSDERSQRIKERMKKYGHDIDFSNLIYEGERSHVVECLTSLGWQVSALTMRDA
                     YAANGFQFPENEALATFADLTYVSAEFG"
     gene            complement(724..2058)
                     /locus_tag="VF874_19810"
     CDS             complement(724..2058)
                     /locus_tag="VF874_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX9178220.1"
                     /translation="MSAKEVTLGIDIGTTAVKAVAADEDGRVVARTRIPHRLRVPEPD
                     RLEHDADEAWRRGPTAALGEMARPDALAVAVSAMVPSMTAVDPDGRPVTPGLLYGDSR
                     GHTAAARAGEFLAGETAEFLHWTAREAPDATGYWPAPAVANHALAGEAVIDVATAGTA
                     FPLFDGTGWSADACAECGASVDRMPKVAMMGAPVGRVRRTGTVLAAGAIDALCEQLVA
                     GADRDGDVLVLCGTTLIVWTTIPEAIQVPGLWTIPHIAAGKSRIGGASNAGGLFLNWV
                     DRVVAPGDSTTTDPRRVPVWSPYVRGERTPFHDPDRRGVLDGLDLTHDGAALRRAAYE
                     ASGFVVRHLIELSGVPVSRVVATGGGTRVEPWMQAIADATGRPVEVSAVAEGAALGAA
                     FLARMAVGLESSMSDAARWAATGHVVEPDPVCAAATQDRYGRFLELANRTPR"
     gene            2089..3063
                     /locus_tag="VF874_19815"
     CDS             2089..3063
                     /locus_tag="VF874_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9178221.1"
                     /translation="MTGLRVLVTAPLRGPGLDKLRRLAEVVYDPWIDQFPLRIYSAEQ
                     LADRVRAEAADIVVVEGDSVGSPVFDLGVRAVASTRGDPSNVDVDGATRAGIPVLHTP
                     GRNADAVAEMAVALLFAATRHIVAADTDVRAGEVFRDGTIPYQRFRAWDLAGRTAGLV
                     GLGAVGRALKWRLAGLGLSVIAHDPYNDEARHSLDELLSEADVISLHAPVTDDTVGMI
                     GTAQFAAMRDGVVFLNTARAQLHDTDALVEALRSGKVAAAGLDHFVGEWLPSDHPLTA
                     LPNVVLTPHIGGATWNTEARQAQMVADGLEALLAGRRPAHIVNPEVLG"
     gene            3060..3716
                     /locus_tag="VF874_19820"
     CDS             3060..3716
                     /locus_tag="VF874_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEX9178222.1"
                     /translation="MRFVDNAETAVLSAAKDMLRRGLVEGTAGNISARRQDGNLVITP
                     SSVDYAEMALDDLVVVDPDGAVLQAAYGRSPSTEMQLHLACYRAFDDIGSVIHSHPVW
                     ATMFAVARQSIPACIDEFAVYCGGDVLCAEYAASGTPDVGNNAVNALQGRAAALIANH
                     GLVAVGPRPDRALHITALVERSAQIVWGAKALGGTVPIPDEVNQNFAGVYTYLRANPM
                     "
     gene            complement(3940..5712)
                     /gene="sppA"
                     /locus_tag="VF874_19825"
     CDS             complement(3940..5712)
                     /gene="sppA"
                     /locus_tag="VF874_19825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX9178223.1"
                     /translation="MFSFLPPVPGLDDLRSLGRKVDTARHHGVPNGGVLELDLLAAPP
                     ETGGFDPVAMITGGGRPLLLREAVAAIHRAAEDPRVSGLIARVQIPAAPAGPVQELRE
                     AIAAFTAVKPSLAWAETYPGTLSYYLASAFGEVWMQPSGTVGLVGFAAHALFLRGALD
                     KAGVEAQFVARGEYKSAANLFTQDSYTDAHREADTQLVESLHSQVWQAIAESRKLDVA
                     DVDALADRAPLLRDDALAGKLVDRVGFRDEAYARIAELVGADTQSDPEEEPPRLYLAR
                     YARAHRPGLPSPKVPGRKSKPTIAVVTVAGPIVSGRGGPRALPFGAHSAGGDTIAAGL
                     REAAADDDVAAVVLRVDSPGGSVTGSETIWREVGRIRGEGKPVVASMGAVAASGGYYV
                     SMGADAIVANPGTITGSIGVVTGKLVARGLKDRLGVGSDAVRTNANADAWSIDAPFTK
                     EQRAQVEAEADLFYDDFVKRVADGRNLSVEDVDEVARGRVWTGADALERGLVDELGGL
                     RTAIRRAKILAGLDADEDAHIVSYPGSSLMDFVRPQQSSQPAAASLPEAVAVVLGRSV
                     AGLVDQAERTFNGASVLWLGDHRF"
BASE COUNT          871 a         2060 c         1947 g          879 t
ORIGIN      
        1 accggctcgt caacggtgag atcgatttgg cagagttgga cccggaattc agcctgcgcc
       61 ggtccgccga gggcatggcc gtgcgcaccc gcttcttcga ccgcatgttc ctcgacgccg
      121 gcacgatggg tgttcgtcag gcggtgatcc tggccgcggg ccttgacgcg cgggcgtacc
      181 ggttgccgtg gccggacggc acggtcgtgt acgaggtcga ccagccggag gtcatcgaat
      241 tcaagacgag cacgctggcc gagctcgggg ccaagcccgc cgccgtccac cgtccggtcg
      301 cgatcgacct ccgcaacgac tggcgtaacg cgttgctaga caatggtttt gaccccacag
      361 cgccgaccgc gtggagcgcc gaggggctgc tgatctatct acctccgcag gcccaggacc
      421 agctgttcga cacgatcacc gagctcagcg cgcggggcag ccggctggcc accgagcaca
      481 tcccggacac gaatgtcttc tccgacgagc gctcgcagcg catcaaagag cggatgaaga
      541 agtacgggca tgacatcgac ttctccaacc tcatctacga gggtgagcgc agccacgtcg
      601 tcgagtgcct gacctcgctc ggctggcagg tgtccgcgct gacgatgcgg gacgcctacg
      661 ccgcgaacgg atttcagttc ccggagaacg aagcgctggc gacgttcgcc gacctcacct
      721 acgtcagcgc ggagttcggt tagccagctc gaggaaccgg ccgtagcggt cctgcgtcgc
      781 agccgcgcac acggggtcgg gttcgacgac gtgcccggtg gcggcccatc tggcggcgtc
      841 gctcatcgac gactccaggc cgaccgccat ccgggcgagg aacgcggctc ccagcgccgc
      901 cccctcggcg accgccgaca cttccaccgg ccgcccggtc gcgtcggcga tcgcctgcat
      961 ccacggctcg acgcgagtgc cgccgcccgt cgccacgacg cgcgacaccg gcactccgct
     1021 gagctcgatc aggtggcgga cgacgaatcc cgacgcctcg taggcggccc ggcgcaacgc
     1081 cgcgccatcg tgggtgagat ccaacccgtc gagcacgccg cgccgatccg gatcgtggaa
     1141 cggggtgcgc tcgcctcgca catatggcga ccacaccggc acccggcgag gatccgtcgt
     1201 cgtcgaatca cccggcgcca caacacgatc cacccagttg aggaacagcc ctccggcgtt
     1261 gctcgcgccg ccgatgcggc tcttgccagc cgcgatgtgc gggattgtcc acagccccgg
     1321 cacctgtatg gcctcgggaa tcgtcgtcca gacgatcagg gtcgtcccgc acagcaccag
     1381 cacgtcgccg tcgcggtcgg ctccggcgac aagctgttcg cacaacgcgt cgatggcacc
     1441 cgcggccaac acggtgccgg tcctcctcac ccggccgacg ggtgcgccca tcatcgcgac
     1501 cttcggcatg cggtcgaccg aagctccgca ctccgcacaa gcgtccgcgc tccacccggt
     1561 gccgtcgaac agcggaaagg cggtgccggc agtcgcgacg tcgatcaccg cctcaccggc
     1621 cagcgcatgg ttggccaccg ccggggctgg ccagtagccg gtggcatcgg gtgcctcccg
     1681 cgccgtccag tgcaggaatt cggccgtctc gcccgcgagg aattccccgg cgcgtgctgc
     1741 cgctgtgtgt ccccggctgt cgccgtagag caaccctggg gtgaccggcc gaccatccgg
     1801 gtcgaccgcg gtcatcgacg gcaccatcgc cgacaccgcc accgccagag cgtcgggccg
     1861 ggccatctca cccagggccg ccgtcggacc gcgccgccac gcctcgtcgg cgtcgtgctc
     1921 caggcggtcc ggctcgggta cccgcaaccg gtgcggaatg cgtgtccgtg ccaccacccg
     1981 gccgtcctcg tccgcggcca ccgccttgac ggcggtggtc ccgatgtcga tgccgagtgt
     2041 gacctctttg gctgacacgc gcgtcaccgt acgccagcat gtgcagacgt gaccggactt
     2101 cgcgtgctcg tgacggcccc gctgcgcggc cctggcctgg acaagttgcg ccggctggcc
     2161 gaggtggtct acgacccgtg gatcgaccag ttcccgctgc gcatctacag cgccgagcaa
     2221 ctggctgatc gagtgcgcgc cgaagccgcc gatatcgttg tggtggaagg tgattcggtc
     2281 ggcagcccgg tgttcgacct cggtgtgcgg gccgtcgcgt ctacgcgcgg ggaccccagc
     2341 aacgtcgacg tcgacggcgc cacgcgcgcg gggattccgg tgctgcacac gcccgggcgc
     2401 aacgccgacg cggtcgccga gatggcggtc gcgctgctct tcgcggccac ccgccacatc
     2461 gtcgccgccg acaccgacgt ccgggccggt gaggtgtttc gcgacgggac gattccttac
     2521 cagcggttcc gcgcatggga cctcgcgggg cgcacggccg gcctagtggg cctgggcgcc
     2581 gtcggccggg cgctgaagtg gcggctggcc gggctggggc tgtccgtcat cgcccacgac
     2641 ccgtacaacg acgaggcgcg gcacagcctg gacgagctcc tctccgaggc cgacgtgatc
     2701 tccctgcacg cgcccgtcac cgacgacacg gtcggcatga tcggcaccgc gcagttcgcc
     2761 gcgatgcgcg acggcgtggt gttcctcaac accgccaggg cgcagctgca cgacaccgac
     2821 gcgctggtgg aggcgctgcg cagcggcaag gtggctgccg cgggcctcga ccatttcgtc
     2881 ggggaatggc tgccgagcga tcacccgctg acggctctgc cgaacgtggt gttgacgccg
     2941 cacatcggtg gggccacgtg gaacactgaa gcgcggcagg cgcagatggt cgccgacggc
     3001 ttggaggcgc tgctggccgg ccgcaggccc gcccacatcg tcaacccgga ggtgctcggg
     3061 tgagattcgt cgacaacgcg gagacggctg tgctgtccgc ggccaaggac atgttgcgac
     3121 gcgggctggt ggagggaacg gcgggcaaca tttccgcccg gcgccaggac ggcaacctcg
     3181 tcatcacccc gtcgtcggtc gactacgcag agatggcgct cgacgatctc gtggtggtcg
     3241 acccggacgg cgctgtgctg caggctgcgt acgggcgatc cccgtcgacg gagatgcagt
     3301 tgcacctggc gtgctaccgg gcgttcgacg acatcggcag cgtgatccac agccatccgg
     3361 tctgggcgac gatgttcgcc gttgcgcgcc aatcgattcc ggcctgcatc gacgagttcg
     3421 ccgtatattg cggcggcgac gtgctgtgcg cggaatacgc cgcgtcgggt acgccggatg
     3481 tcggcaacaa cgcggtcaac gcgcttcagg gcagggcggc ggcgctgatc gcgaaccacg
     3541 gcctggtggc ggtcggaccc cggccggacc gggctttgca tatcactgca ttggtcgagc
     3601 ggtcggctca aatcgtctgg ggcgcaaagg ctctcggtgg gacggtgccg attcccgacg
     3661 aggtcaacca gaacttcgcg ggcgtctata cgtacctgcg cgcgaacccg atgtagctcg
     3721 ttcccgcgca ccgccgacgg cctctgcccc gtcggcgctt gccacatccg taacatgctg
     3781 gggcgcaacc cttttctgcc cgcctctcag cgacaagccc ctaacctgca tcgttagcgt
     3841 gaattgtcgc tgatcgcgtt atccccgtta tggcgtttga cctgcacata tgctgttgtc
     3901 gctcactggc gggcacgaag gtgtgttcca ccgctggggt cagaagcggt gatcacccaa
     3961 ccacagcacg ctggcgccgt tgaacgtgcg ctcggcctga tcgacgagcc cggccaccga
     4021 tcgcccgagc accacagcga ccgcttccgg cagcgacgcg gccgccggct gcgacgactg
     4081 ttgcggccgc acgaaatcca tcaacgacga accggggtag ctgacgatgt gggcgtcctc
     4141 gtcggcgtca aggccggcga ggatcttcgc gcgcctgatc gccgtgcgca ggccgcccag
     4201 ctcgtcgacc agaccacgct cgagcgcgtc cgcgcccgtc cacacccggc cgcgagcgac
     4261 ctcgtcgacg tcttcgaccg aaaggttgcg tccatcggcc acccgcttga cgaagtcgtc
     4321 gtagaacaag tctgcttcgg cttcgacctg cgcgcgctgt tccttggtga acggcgcgtc
     4381 gatggaccag gcatcggcat ttgcgttggt gcgcaccgca tctgacccca cgcccaggcg
     4441 atccttcaga ccgcgggcca ccagcttgcc ggtcacgaca ccgatcgaac cggtgatggt
     4501 gcccggattg gcgacgatcg cgtccgcgcc catcgacacg tagtagccgc cggaggcggc
     4561 gaccgccccc atcgatgcga cgaccggctt gccctcgccg cgaatccggc cgacttcgcg
     4621 ccagatggtc tccgacccgg tgacggaccc accggggctg tcgacgcgca gcaccactgc
     4681 ggcgacgtcg tcgtcagcgg cggcctcgcg cagcccggcc gcgatcgtgt cgccgccggc
     4741 gctgtgggcc ccgaacggaa gagcacgcgg gccgccgcgg ccgctgacga tcggccctgc
     4801 cacggtcacc accgcgatcg tcggcttgct tttgcgccca ggcactttcg gcgacggcag
     4861 ccccggccgg tgggctcggg cgtagcgagc gagatagagc cgcggcggtt cttcttcagg
     4921 atcagactgg gtatcggcgc cgacgagttc ggcgatgcgc gcgtacgcct cgtcgcggaa
     4981 gccgacccgg tcgaccagct tgccggccag cgcgtcgtcg cgcagcaacg gcgcccggtc
     5041 ggccagcgcg tcgacgtcgg cgacatcgag cttgcgcgac tcggcgatcg cctgccacac
     5101 ctgactgtgc aggctttcca ccagctgtgt gtcagcctcg cggtgcgcgt cggtgtagct
     5161 gtcttgcgtg aaaaggtttg ccgcagactt gtattcaccg cgcgcaacga attgcgcctc
     5221 gacaccggcc ttgtccagcg cgccgcgcag gaacagtgcg tgagcggcga aaccgaccag
     5281 gccgacggtg cccgacggct gcatccacac ctcgccgaat gcggaggcca ggtaatacga
     5341 cagcgtgccc gggtacgtct cggcccacgc cagcgacggc ttgacggcgg tgaatgcggc
     5401 gatggcctcg cgcagttcct gcaccggacc ggcgggggcg gcgggaatct gcacgcgggc
     5461 gatcagcccg cttacccgag ggtcctcggc agcgcggtgg atcgcggcga cggcctcgcg
     5521 cagcagcagg gggcggccgc cgccggtgat catcgcgacc gggtcgaagc ccccggtctc
     5581 tggcggtgcc gccagcagat ccagctccag cacgcccccg ttcggtacgc cgtgatgacg
     5641 ggcggtgtcc accttgcggc ccagtgaccg caggtcgtcg aggcccggaa cgggcggcag
     5701 gaaactgaac atgccccaag cctattcgtt gagccatgcg cggttgccgt acccgtc
//
LOCUS       DASWBF010000531         5188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_571551,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000531 DASWBF010000000
VERSION     DASWBF010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571551"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1774)
                     /gene="nuoN"
                     /locus_tag="VF874_19830"
     CDS             complement(191..1774)
                     /gene="nuoN"
                     /locus_tag="VF874_19830"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX9178224.1"
                     /translation="MSIPAPSVEYALVSPMLIVFSAAVAGVLVEAFAPRRMRYPVQLV
                     LSLGASLAALVAVVVVAGDLLLPGHSTIAGSVAVDGPTLLLQGTLLVVGILGLMLIAE
                     RRIGAEKVAVEAVSAGAGFDAFTPQASAIPHSPAEREADRAGVSQTEVFPLTLFALAG
                     MLLFPASNDLLTMFVALEVLSLPLYLLCGLARRRRLLSQEAALKYFLLGAFSSAFFLY
                     GVALLYGYAGTLTLSGIADAISAQHGDQSMALIGVALLSVGLLFKVGAVPFHSWIPDV
                     YQGAPTPITAFMAAATKIAAFGATLRIFYVALPGMQEQWRPVMWAIAILTMAVGTVSA
                     VTQSDVKRMLAYSSVAHTGFILTGVIAANPAGLSSTMFYLVAYGFSTLGAFAVVSLVR
                     NADGDEDTDMSRWAGLGRERPLVGAMFSMFLLAFAGIPLTSGFVAKFAVFRAAAQGGA
                     APLVIVGVVASGVAAYFYVRVIVLMFFTEPAADPPQVVVPSALSKIAIAISAAATIVL
                     GIMPQPLLDLCDSAAQFIR"
     gene            complement(1771..3348)
                     /locus_tag="VF874_19835"
     CDS             complement(1771..3348)
                     /locus_tag="VF874_19835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX9178225.1"
                     /translation="MVVNIPWLSILWLLPVAGTVVTMLLPPAQRAQAKWVGLVVSLAV
                     LAVAVVLAISFNTNGPQYQFTESRQWIPAFGTGYTLGLDGIALALVLLTAVLVPLLLV
                     AGWNDAGERKRAAHTYVALILAVEAMVLISLVALDVLLFYVFFEAMLIPMYFLIGGFG
                     SGEQRSKAAVKFLLYNLFGGLIMLAAVIGLYVVTTHSGAGTFDFREIVAWVSSGQLGV
                     DPVVLNALFLGFMFAFAIKAPLWPFHAWLPDAAVQSTPATAVLMMAVVDKVGTFGMLR
                     YCLQLFPDASKMFAPLIITLAVVGIVYGAILAIGQTDVMRLIAYTSISHFGFIILGIF
                     VMTSQGQTGSTLYMVNHGISTAALFLIAGFLVSRRGSRAIASFGGVQKVAPVLAGTFL
                     VSGLATLSLPGLAPFISEFLVLIGTFTRYQAAAVIASIALVLSAIYVLWMYQRMMTGP
                     VKEGNERLRDLLPREIAVVAPLIALLLVLGIYPKPALDLIDPAVGHTLTTIGQHDPAP
                     SVTPVAALNPDAEGQHK"
     gene            complement(3339..>5188)
                     /gene="nuoL"
                     /locus_tag="VF874_19840"
     CDS             complement(3339..>5188)
                     /gene="nuoL"
                     /locus_tag="VF874_19840"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9178226.1"
                     /translation="GAVVLLLGGRRTDRWGHLLGCATVLGAFAVGVVLFADLLGRQGE
                     DRAIHLDGFSWVPVGGLQVDFGLLVDPLSMCFVLLITGVGSLIHIYSIGYMAEDLDRR
                     RFFAHLNLFVAAMLLLVLADNYLGLYVGWEGVGLASYLLIGFWYHKPSAATAAKKAFV
                     VNRVGDIGLAIAMMVMFATFGTISFSGVFGATGSASEGVLTALGLLLLLAACGKSAQV
                     PLQSWLGDAMEGPTPVSALIHAATMVTAGVYLIVRSHPIYNLAPGAHAGVVTVGAVTL
                     LFGAIIGCAKDDIKKALAASTMSQIGYMVLAAGLGPAGYAFAILHLLTHGFFKAGLFL
                     GAGSVMHAMDDEVNMRRYGGLRTLLPITYVTFGLGYLAIIGVPPLAGFFSKDAIIETA
                     LAAGGFKGAVLGGAALLGAGITAFYMTRVMLMTFFGERRWESTDVHPHESPRVMTWPM
                     IVLAVGSVASGGLLAIGGTLQHWLEPVVGTHEEAAHVMPVWVTTTITLAVVAVGIAIA
                     YRLYATQPVPAEAPAGSALTVAARNDLYGDALNEEVFMRPGADLTRTLVRVDDKGIDG
                     TVTGLAAAIGRLSGTLRQAQTGFARSYALSMFAGAALVVAAILAVNLWW"
BASE COUNT         1035 a         1755 c         1692 g          706 t
ORIGIN      
        1 ctcggccatc ggactcggca cgctaggtct cgtcggttcg gcgaacgccg caccggccac
       61 cgagcagccc accggctgga cggtgtacac ctgctacgac tacgtcaccc agacgttcta
      121 cgactgcgtc ggctgacccc agcgaatggg cccaccctca agaaagcggg ggcgggccca
      181 ttctcgctgt tcagcgtatg aattgcgctg cgctgtcgca caagtcgaga agcggctgcg
      241 gcatgatgcc gagcacgatg gtagccgcgg ccgaaatcgc gatcgcgatc ttgctcagcg
      301 cgctcggcac caccacctgg ggcgggtcgg cggccggctc cgtgaagaac atcagcacga
      361 tcactcgcac gtagaagtag gccgcgacgc cgcttgccac cacgccgacg atcaccagcg
      421 gcgcggcacc accttgggcg gccgcccgga acaccgcgaa cttggcgacg aagccgctcg
      481 tcagcggaat gccggcgaac gcgagcagaa acatcgagaa catcgctccg accaacggac
      541 gctcacggcc caaacccgcc cagcgtgaca tgtcggtgtc ctcgtcgccg tccgcgttgc
      601 gcaccagcga cacgaccgcg aacgcgccca gcgtgctgaa cccgtacgcc acgagataga
      661 acatcgtcga cgacaggccg gccggattcg ccgcgatcac gccggtgaga atgaacccgg
      721 tgtgcgcgac cgacgaatag gccagcatcc gcttcacgtc ggattgcgtg acggcgctga
      781 cggtcccgac ggccatcgtc aggatcgcga tcgcccacat caccgggcgc cattgctcct
      841 gcatacccgg cagcgcgacg tagaagatcc gcagcgtcgc gccgaacgcg gcgatcttgg
      901 tggcggcggc catgaaggcc gtgatcggcg tcggcgcacc ctgatacacg tcggggatcc
      961 aggagtggaa cggaaccgcg ccgactttga acagcaggcc caccgacagc agcgcgacgc
     1021 cgatcaacgc catggattga tcgccgtgct gcgcgctgat cgcgtcggcg atccctgaca
     1081 gcgtcagcgt gccggcgtat ccgtacagca gcgccacgcc gtacaggaag aaggccgacg
     1141 aaaacgcgcc caacagaaag tatttcagcg ccgcttcctg cgacagcagc cggcgccgtc
     1201 gcgccaggcc gcaaagcagg tacagcggca gcgacagcac ttccagagcg acgaacatcg
     1261 tcagcagatc gttggatgcc gggaacagca gcatcccggc aagcgcgaac aacgtcagcg
     1321 ggaacacttc ggtctggctg accccggccc ggtccgcctc gcgctcagcc gggctgtgag
     1381 gtatggctga agcctgcggc gtgaacgcgt cgaagccggc gcccgccgaa accgcttcga
     1441 ctgcaacctt ttccgcgccg atgcggcgtt cggcgatcag catgagaccg aggattccaa
     1501 ctaccagcag ggtgccctgc agtagcagcg tcggaccgtc aaccgccacc gaacccgcaa
     1561 tggtgctgtg cccgggcagg agcagatccc cggcgacgac cacgactgcc accagcgccg
     1621 ccagcgaagc gccgaggctc aggacgagct gcaccgggta gcgcattcgg cgcggcgcga
     1681 acgcttcgac gagcacgccc gcgacggccg cgctgaacac gatcagcatg ggtgacacga
     1741 gggcgtactc gacgctcggc gcgggaatgc tcatttgtgc tgtccctctg catccggatt
     1801 cagcgccgcc accggcgtga ccgacggagc ggggtcatgt tggccgatcg tggtcagcgt
     1861 gtggccgacg gcggggtcga tgaggtcgag cgcgggtttg gggtagatgc cgagcaccag
     1921 cagcagcgct atcagcggtg cgacgacggc aatctcgcgg ggcagcaggt cgcgcagccg
     1981 ctcgttgccc tccttgaccg ggccggtcat cattcgctga tacatccaca gcacgtagat
     2041 cgccgacagc accagggcga tggaggcgat cacggcggcg gcctggtagc gcgtgaatgt
     2101 cccaatgaga acgaggaatt cgctgatgaa cggtgccagg ccgggcagcg acagcgtcgc
     2161 cagaccggac accaggaagg tgcctgccag cacgggtgcg actttctgca ctccgccaaa
     2221 tgaggcgatc gcgcggctcc cgcgccgcga gaccaagaac ccggcgatca ggaacagcgc
     2281 cgcggtcgag atgccgtggt tgaccatgta gagcgttgac ccggtctggc cctggctggt
     2341 catcacgaag atgcccagga tgatgaaccc gaagtgcgag atcgacgtgt aggcgatcag
     2401 ccgcatcacg tcggtctgcc cgatcgccag gatcgcgccg tagacgatgc cgaccacggc
     2461 cagcgtaatg atcagcggcg caaacatttt cgacgcgtcg gggaacagct gcagacagta
     2521 ccgcagcatg ccgaacgtgc cgaccttgtc gaccacggcc atcatcagca cggcggtggc
     2581 cggggtggac tgcacggccg cgtcgggcag ccaggcatgg aacggccata gcggcgcctt
     2641 gatcgcgaac gcgaacatga agcccaggaa cagcgcgttg agcacgacgg gatcgacgcc
     2701 gagctgaccg gacgacaccc acgcgacgat ttcgcggaaa tcgaaggtgc ccgcgccgct
     2761 gtgcgtcgtc accacgtaca acccgatcac ggcggcgagc atgatcagcc cgccgaacag
     2821 gttgtacagc aggaacttca cggctgcctt cgaccgctgc tcgccgctgc cgaagccgcc
     2881 gatcaggaag tacatcggga tgagcatggc ctcgaagaac acatagaaca gcagcacgtc
     2941 aagcgcgaca agcgatatca gcaccatcgc ttcgacggcc aagatcagcg ccacgtaggt
     3001 gtgggcggct cgtttgcgtt cgccggcgtc gttccagccg gccaccagca gcaatggcac
     3061 cagaacggcc gtcagcagga cgagcgccaa cgcgatgccg tccaggccga gcgtgtagcc
     3121 ggtgccgaaa gccggtatcc actggcggga ttcggtgaac tggtattgcg gcccgttggt
     3181 attgaacgag atcgccagca cgacggccac tgccagcacc gccagcgaca cgacgagtcc
     3241 cacccacttg gcctgtgcgc gttgcgccgg gggcagcagc atggtcacga cagtgccggc
     3301 caccgggagt agccacagga tggaaagcca gggaatgttc accaccacag gttcaccgcc
     3361 agaattgcgg cgacgacgag cgcggcgccg gcgaacatgg acaacgcata ggaacgggcg
     3421 aacccggttt gggcttggcg cagcgttccg gacaatcgac cgatcgccgc cgccagtccg
     3481 gtgacggtgc cgtcgatgcc cttgtcgtcg acgcggacca aggtgcgggt caggtcggcg
     3541 ccggggcgca tgaacacttc ttcgttgagc gcgtcgccgt agaggtcgtt gcgggccgcg
     3601 acggtcaacg ccgacccggc aggtgcctcg gcgggcaccg gctgcgtagc gtacaagcga
     3661 taggcgatgg cgatgccgac agcgaccacg gcaagcgtga tggtggtggt gacccacacc
     3721 ggcatcacgt gagccgcctc ttcgtgcgtg ccgacgactg gttcgagcca gtgctgcagt
     3781 gtgccgccga tcgcgagcag cccaccggag gcgaccgacc cgaccgcgag cacgatcatc
     3841 ggccaggtca tcacccgcgg cgattcatgc gggtgcacgt ctgtgctttc ccaacgccgc
     3901 tccccgaaga acgtcatcag catcacgcgg gtcatgtaaa acgcggtgat gcccgcgccc
     3961 agcagcgccg cgccgccgag caccgcacct ttgaatccgc ctgccgccaa cgcagtttcg
     4021 atgatcgcgt ccttggagaa gaacccggcc agcggtggca ccccgatgat cgccagatag
     4081 ccgagcccga acgtcacgta ggtgatcggc agcagggtgc gcagcccgcc gtagcggcgc
     4141 atgttcactt cgtcgtccat tgcgtgcatc accgacccgg cgcccaggaa cagcccggcc
     4201 ttgaagaagc cgtgggtgag caggtgcagg atcgcgaacg cgtacccggc ggggcccagc
     4261 ccggcggcaa gcaccatgta cccgatctgg ctcatcgtcg acgcggccag agccttcttg
     4321 atgtcgtctt tggcgcaacc gatgatcgcg ccgaacaaca gcgtgaccgc gccgacggtc
     4381 acgacgccgg cgtgcgcgcc gggcgcgagg ttgtagatgg gatgagaccg cacgatcagg
     4441 tagacaccgg cggtcaccat cgtggcggcg tggatcagcg ccgacaccgg agtggggcct
     4501 tccatcgcgt cgccgagcca ggactgcagc ggcacctggg cggacttgcc gcacgcggcg
     4561 agcagcagca gcaacccgag tgcggtcaac acgccctcgc tcgcggatcc ggtcgcgccg
     4621 aacacgccgg aaaacgagat cgtgccgaag gtggcgaaca tgaccatcat cgcgatggcc
     4681 aggccgatgt cgccgacgcg gttgaccacg aacgcttttt tcgcagcggt ggccgccgac
     4741 ggcttgtggt accagaaccc gatcagcaga tacgatgcca agccgacgcc ttcccacccg
     4801 acgtacaggc cgaggtagtt gtcggccagc accagaagca gcatcgctgc gacgaacagg
     4861 ttcaggtgcg cgaaaaacct tctgcggtcg agatcctcgg ccatgtagcc gatcgagtag
     4921 atgtggatca gcgagccgac gccggtgatc aacagcacga agcacatcga cagcgggtcc
     4981 accaaaagcc cgaagtccac ctgcaacccg ccgacgggca cccaggagaa cccgtccaga
     5041 tggatcgcgc ggtcctcgcc ctggcggccc agcaggtcgg cgaacagcac caccccgacc
     5101 gcgaacgcgc cgagcacggt cgcacacccc agcaggtgtc cccaccggtc ggtgcgacgg
     5161 ccccccaaca gcagcaccac cgccccga
//
LOCUS       DASWBF010000532         8157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_571695,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000532 DASWBF010000000
VERSION     DASWBF010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571695"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VF874_19845"
     CDS             complement(<1..742)
                     /locus_tag="VF874_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX9178227.1"
                     /translation="MTQPETTPETTRPGLLVEIKEIATTVAVPYHEPPAVVLRRRIVV
                     AVFLVAGAVVLGFSLNRPPGDDSFYWATFLLAGIWFIGALLSRPLHLGSIKFRGRNQR
                     PVITGTLVGLGLGGLFLLGGLVAREIPAVSEHIKNVLEFADKGSLQLVLLITLINGIA
                     EECFFRGALYSALGRFHPVLISTLLYTAATLASGNPMLGFAAVILGTVCALERRATGG
                     VLAPILTHFWWSALMVLALPPIFGLSAGQ"
     gene            complement(739..1878)
                     /locus_tag="VF874_19850"
     CDS             complement(739..1878)
                     /locus_tag="VF874_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX9178228.1"
                     /translation="MRILVTGATGYVGSRLVATLLADDHRVVAATRNPARLKHFGWYR
                     DVDAVALDAADLDATRAAFSDSGPVEVVYYLVHGIGQPGFRDADNAAAANFAIPAKEA
                     GVRRIVYLGGFVPEDDDLSEHLTSRAEVAHSLCVDGGPEVVWLGAAMVVGAGSTSFEM
                     LRYVGDRFLLFPLLPWVENPVDPISIRDVLYYLVAVADSDRVPPGGYDICGPERTTYR
                     RLLFGYARATGTWRAGLPIRGIDTALVARVTAVALPVPGGLAADLVESLDHPMMASEH
                     RLRDLVPDPPGGLTPIDDAIAASVATRKLRPVNELADPHHLADSDPDWAGGDVLALQR
                     LARAVMPTAARPAIELVNLVPGPVAGVLRTGIDILADLTPNGKSA"
     gene            1965..2315
                     /locus_tag="VF874_19855"
     CDS             1965..2315
                     /locus_tag="VF874_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178229.1"
                     /translation="MRLATLRCGERDLPLQFTNHFLAHLQAAVHRRFKKHGGFFLTGT
                     YQNDDGSDVTVSHWVHPLVPLEFHYDVRDDSGNRLPPVELDHKEIDAILDAMDRPVGV
                     HDTGDVWLSFRDKL"
     gene            complement(2312..2836)
                     /locus_tag="VF874_19860"
     CDS             complement(2312..2836)
                     /locus_tag="VF874_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX9178230.1"
                     /translation="MPEPLIVTVRGRTPQIDPEAWIAPNASVIGQVTLSARASVWYAA
                     TLRAEAEPIHVGAGTNIQDGVTVHVDSEYPVHIGTGVSVGHNAVVHGCEVEDHVLIGM
                     GAIIMNGAVIGTCSLVAAGTLVPQGMVVPPRSLVTGVPGRVRRELSDAEVVGNQQNAG
                     IYERLAELHRAAVT"
     gene            complement(2847..3638)
                     /locus_tag="VF874_19865"
     CDS             complement(2847..3638)
                     /locus_tag="VF874_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9178231.1"
                     /translation="METEPAALVERRGNVEVITINRPEARNAVNGAVSIAVGDALERA
                     NDDPDVWAVVITGAGDKSFCAGADLKAISRRENLYHPDHGEWGFAGYVHHFIDKPTIA
                     AVNGTALGGGTEIALASDLVVADERATFGLPEVKRGLVAAAGGVFRIVDALPRKVAFE
                     MMFTGDPISAADAARWGMINQVVPDGTTLQAALAMAARVTVNAPLAVQVSKRVGYGVD
                     DGVVTDEEPKWARLMREVKSLLKSEDAKEGPLAFAEKRQPVWKAR"
     gene            complement(3644..4789)
                     /locus_tag="VF874_19870"
     CDS             complement(3644..4789)
                     /locus_tag="VF874_19870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9178232.1"
                     /translation="MPEAVIVEAIRSPVGKRNGGLSGVHPADLSAQVLNALVERAGVD
                     PEIVDDVIWGCVMQAGEQSMDIARTSLLAAGWPESVPGVTVDRQCGSSQQSIHFAAAG
                     VVAGHYDAVVAGGVESMSRVTMGASLAGGGNPFPKGFMDRYGVHPNQGIGAEMIADQW
                     GLDRTTLDEFSLGSHEKAAAAQDSGAFDDQIVATKDQDGNPVLRDEGIRRGTPMEKMA
                     QLKPAFKEDGVIHAGNSSQISDGSAAVLITTHEKAKSLGLKPIAKIHTAVLAGANPVI
                     MLTAPIPATQKALKKSGLNIGDIGVFEVNEAFAPVPLAWLHDIGADEKNLNPNGGAIA
                     LGHPLGGSGARIMTTMLHHMRDKGIRYGLQTMCEGGGQANATIVELL"
     gene            4943..6331
                     /locus_tag="VF874_19875"
     CDS             4943..6331
                     /locus_tag="VF874_19875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9178233.1"
                     /translation="MTTLDRAFAVNSPRLTPRGAAVAGWSIHIAVAALWVALFAQVFL
                     QVGAGLWSIGIAYIAYDTLLLVFVLWQTLPLRRATRPAPVSGGPTVGVLIAAYNEAPV
                     LPATLRALLAQADPPEQIVVADDGSDDGTAAALEAEFGDRHHPSLQLLKLAHGGKAVA
                     LNAALMAVDTDVVVTMDADTLPAPDAIAAIRNAFAADPDLVVAGGVLTPMCGASLRNR
                     ILQWFQTYEYLRNFVGRYAWMRVNCLLLISGAFAGFRRNAVLSVGGFDTDCLVEDYEL
                     IHRLQRRSRDDGLGWRVQIVGNAVAQTDAPGTIRDFLRQRRRWFGGFLQTQYWNRDMT
                     GNPRYGALGLAMLPVKAVDTVQPIYGLTAFVVLIVLAVTGNAAVLIPVGAIVLTKLLV
                     DLFAYVWTIHLYRRWTGSDANTHFGAGLLAAFVEPFTFIPLLHTCALLGWWQFLTGRQ
                     TWVPQRRRGIVA"
     gene            6354..6770
                     /locus_tag="VF874_19880"
     CDS             6354..6770
                     /locus_tag="VF874_19880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178234.1"
                     /translation="MNTDAVGVHGVSTNHDGVQGETTHPDRAGVTGTNLSGNGVFGQS
                     QAVNAFGVCGINTAGVGVRGDSEDHDGVQGFANNTDKAGVVGTHLGSGVGVRGQSVFG
                     TGVHAASENSIGLEASGSIAARLVGKPSSKRPRRHR"
     gene            6772..7182
                     /locus_tag="VF874_19885"
     CDS             6772..7182
                     /locus_tag="VF874_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178235.1"
                     /translation="MFLSGADYAEALSTTDESVREGVVVVLGHDGQVHPCQQDYDTAV
                     AGIVSGAGGYKPAIVLDRHENSAHVALMGKVWCLADADIAPIRPGDLLTTSSTTGHCR
                     RVTDTSRAFGAVIGKALSPLPNGRGLIRVLVSAR"
     gene            7309..>8157
                     /locus_tag="VF874_19890"
     CDS             7309..>8157
                     /locus_tag="VF874_19890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178236.1"
                     /translation="MVFDEPIITSDGVDLGGHQTLTVRNDGTFRWQGHMRATGLQSFE
                     FAIITALVHPIALPDGTSATSRIVFAESGEVHGSNKPGDRVFAWDQQSENKLLRSEWL
                     GVRQGRIEHRLEFDNDLFGVVGDVVSFLAQVVALGATFGAAGVALVVAGKAAALLNVD
                     QIVLPGMIGVLFSAGAAFVIGPVAMFPVLVVGAVTAAALIRQREMTPEERAFADQVFA
                     GKIRYDRVRLTNLVGVGERPFTAPGPGETILVNLGKGFDSPTTYTGLGGEATGIRAPG
                     QLLIHEL"
BASE COUNT         1315 a         2750 c         2730 g         1362 t
ORIGIN      
        1 attgccctgc cgagagaccg aaaatcggcg gcaacgccag gaccatgagc gcgctccacc
       61 agaaatgcgt caggatcggg gcgagcacgc cgccggtggc ccggcgttcg agcgcgcaca
      121 cggttccgag gatcacggcc gcgaacccga gcatcgggtt accgctggcc agcgtcgcgg
      181 cggtgtacag cagcgtcgag atgaggacgg gatggaaccg gcccagcgcc gagtacagcg
      241 caccgcggaa gaagcattcc tcggcgatgc cgttgatcag cgtgatgagc aggaccagct
      301 gcagcgagcc tttatcggcg aactcgagga cattcttgat gtgctcgctg accgcgggga
      361 tctcgcgggc gaccaggcca ccaagcaaga acaggccgcc gaggcccagc ccgacgaggg
      421 taccggtgat caccggccgc tggttgcgcc cgcggaactt gatgctgccg agatgcagcg
      481 gccgggacag caacgcgccg atgaaccaga ttccggcgag caggaacgtc gcccagtaga
      541 acgagtcgtc gcccggcggt cggttcaacg agaacccgag caccacggcg ccggccacga
      601 ggaagaccgc gacgacaatc cgccgccgga gcaccaccgc aggcggctcg tggtacggaa
      661 cggcaacggt ggtcgcgatc tccttgatct cgaccagcag cccgggccga gttgtttctg
      721 gtgttgtttc tggttgcgtc atgctgactt tccgttcgga gtgaggtcgg ccagaatgtc
      781 gatcccggtg cgcaacactc cggccaccgg gccgggcacc aggttgacga gttcgattgc
      841 gggccgagcg gctgtcggca tcacggcgcg cgccagccgc tgcagcgcga gcacgtcgcc
      901 gccggcccag tcggggtcgc tgtcggccaa gtggtgcggg tcggccagct cgttgaccgg
      961 gcgcagtttg cgggtcgcca ctgaagcggc gatcgcgtcg tcgatcgggg tcaggccacc
     1021 cggcgggtcc ggcacgaggt cgcgcaaccg gtgctcggag gccatcatcg ggtgatccag
     1081 tgactcgacg agatcggcgg cgagcccacc gggaacgggc agtgcgacgg ccgtcacccg
     1141 cgccaccaga gcggtgtcga tgccgcggat cggcagcccg gcccgccacg tgccggtcgc
     1201 ccgggcatag ccgaacagca gacgccggta ggtcgttctc tccggcccgc agatgtcgta
     1261 gccccccggc ggcacccggt ccgaatctgc cacggcaaca aggtaataca gcacgtcgcg
     1321 gatcgagatc gggtccaccg ggttctcgac ccacggcaag agcgggaaca gcaggaaacg
     1381 atcaccgaca tagcgcagca tctcgaacga cgtcgacccg gcgccgacaa ccatcgcggc
     1441 gccgagccac accacctcgg ggccgccgtc gacacacagc gagtgggcca cctcggcacg
     1501 gctggtcaag tgctccgaca ggtcgtcgtc ctcaggcacg aagccgccga gataaacaat
     1561 gcggcgcacg ccggcctcct tggcgggaat cgcgaaattc gcggccgcag cgttgtcggc
     1621 atcccgaaac cccggctggc cgatcccatg gacgaggtag tagacgacct cgacggggcc
     1681 ggaatcggag aacgccgccc tcgtggcgtc caggtcggcg gcgtcgagcg cgaccgcgtc
     1741 cacatctcga taccagccga aatgcttgag acgggccggg tttcgggtgg ccgccacgac
     1801 gcgatggtcg tccgccagca gtgtcgcaac gaggcgtgaa cccacatatc ctgtggcgcc
     1861 ggtcacgaga attcgcacat tgctcaccgt aggtgattac ccactcccgc cgggacagga
     1921 caggagcttc ctaggctggt ggtgcatcca cgacggaagg agacgtgaga ttggccacgc
     1981 ttcgctgcgg tgagcgagac ctgccgcttc agttcaccaa ccatttcctg gcccacctgc
     2041 aagccgcggt gcaccggcgc ttcaagaaac acggcggctt tttcctgacg gggacctatc
     2101 agaacgacga cggcagcgac gtcacggtct cccactgggt gcatcccctc gttccgctgg
     2161 agtttcatta cgacgtgcgc gacgacagcg gcaaccggct gcctcctgtc gagctggacc
     2221 acaaggagat cgacgcgatc ctggacgcga tggaccggcc cgtcggtgtg cacgacaccg
     2281 gtgacgtgtg gctgagcttc cgggacaagc tctaggtcac cgcggcgcgg tgcagttcgg
     2341 cgaggcgttc gtagatgccg gcgttctgct ggttgccgac cacctcggcg tcgctcagct
     2401 cgcgacgcac gcgccccggc accccggtca ccagcgacct cggcggcacc accatgccct
     2461 gcggcaccag cgtgcccgcc gcgaccagtg agcacgtgcc gatcaccgcg ccgttcatga
     2521 tgatcgcgcc catgccgatc agcacgtggt cctcgacctc gcagccgtgc accacggcgt
     2581 tgtgcccgac gctcaccccg gtaccgatgt gcaccgggta ttcggagtcg acgtggaccg
     2641 tgacgccgtc ctggatgttg gtgcctgctc cgacgtggat cggctctgct tcggcgcgca
     2701 gcgtcgcggc gtaccagaca ctggcccgag cggacagggt cacctgaccg atcacggatg
     2761 cgttcggggc gatccaggct tcgggatcga tctgcggggt gcggccacgc acggtgacga
     2821 tcagcggctc gggcatgctg gcgcccctat cgtgctttcc acaccggttg ccgtttctcg
     2881 gcgaacgcca gcgggccttc cttggcgtcc tccgacttca gcagcgactt gacctctctc
     2941 atcagccgcg cccacttcgg ctcttcgtcg gtgaccacgc cgtcgtcgac cccgtaaccc
     3001 acccgcttgc tgacttgtac cgccagcggc gcattcaccg tgacacgtgc ggccatcgcc
     3061 aacgcggcct gcagcgtggt gccgtcgggc accacctggt tgatcatccc ccagcgggcc
     3121 gcgtcggccg cggagatcgg gtcgccggtg aacatcatct cgaacgccac cttgcggggc
     3181 agggcatcga cgatccggaa cacgccgccg gcagcggcga ccaagccgcg cttgacctcc
     3241 ggcaacccga acgtcgcgcg ctcgtcggcc acgaccaggt cactggcgag cgcgatctcg
     3301 gtgccgccac cgagcgcggt gccgttgacc gccgcgatcg tcggcttgtc gatgaagtgg
     3361 tgcacatagc ccgcgaagcc ccactcgccg tggtcggggt gatacaggtt ctcccgacgc
     3421 gagatcgcct tcagatcggc tccggcgcag aaggatttgt cacccgcgcc ggtgatcacc
     3481 acggcccaca cgtcggggtc gtcgttcgcg cgctccagcg cgtcaccgac cgcgatgctg
     3541 accgcgccgt tgacggcgtt gcgcgcctcc ggccggttga tcgtgatcac ctcgacgttg
     3601 ccgcggcgct cgaccagagc cgcaggctca gtttcaatga acgtcacagc aattccacga
     3661 tggtggcgtt ggcctggccg ccgccctcac acatggtctg caagccgtag cgaattccct
     3721 tgtcccgcat gtgatgcagc atcgtcgtca tgatccgggc acccgacccg ccgagtgggt
     3781 ggcccaacgc gatggcgccg ccgttggggt tgaggttctt ctcgtcggcg ccgatgtcgt
     3841 gcagccacgc cagcggcacc ggcgcgaacg cctcgttcac ctcgaacacc ccgatgtcgc
     3901 cgatgttcag cccggacttc ttcagcgcct tctgcgtcgc ggggatcggt gcggtgagca
     3961 tgatcaccgg gttggcgccg gccagcaccg cggtgtggat cttcgcaatc ggcttgagcc
     4021 ccaacgactt cgccttctcg tgggtcgtga tcagcactgc ggcggacccg tcggagatct
     4081 gcgacgagtt gccggcgtgg atcacgccgt cctccttgaa cgccggcttc agctgggcca
     4141 tcttctccat cggggtgccg cgccggatgc cctcgtcacg gagcacgggg ttgccgtcct
     4201 ggtctttggt cgcgacgatc tggtcgtcga acgcgccgga atcctgtgcg gcggcggcct
     4261 tttcatgcga gcccagcgag aactcgtcga gcgtggtgcg gtcgaggccc cactggtcgg
     4321 cgatcatctc cgcgccgatg ccctggttgg ggtgcacgcc gtagcggtcc atgaagccct
     4381 tcgggaacgg gttgccgcca ccggcgagcg acgcgcccat cgtcacccgc gacatcgact
     4441 cgacgccacc ggccaccacc gcgtcgtaat ggccggccac cacaccggcc gcggcgaagt
     4501 ggatggactg ctggctggac ccgcactgcc ggtcaaccgt cacgccggga acgctttccg
     4561 gccatcccgc cgccagcagc gacgtgcgcg cgatgtccat ggattgctcg ccggcctgca
     4621 tgacgcagcc ccagatcacg tcgtcgacga tctccgggtc gaccccggcg cgctccacca
     4681 gcgcgttgag cacctgcgcg gacaggtccg cgggatgcac acccgacagc ccgccgttgc
     4741 gcttgccgac cggcgaccgt atcgcttcga cgatgacggc ttctggcatg agcttctcct
     4801 atcaaccagt tgacttgtgt cgattgttga ccaggccggt cagcgggtcg cgccgggggt
     4861 cacgatgtca atccaagatg tgacgagtga acttctccgc actccggcgc gtgtccgagt
     4921 caaacctgtg aagctggaga acgtgacgac cttggaccgc gcgttcgcgg tgaactcgcc
     4981 gcggctgacc ccgcggggcg ccgcggttgc gggctggtcg attcacatcg cggtcgccgc
     5041 gctgtgggtg gcgttgttcg cgcaggtgtt ccttcaggtc ggggcgggct tgtggtcgat
     5101 cggcatcgcc tacatcgcct acgacacgct gctgctggtg ttcgtgctgt ggcagacact
     5161 gccgctacgc cgcgcgacgc gccccgcgcc ggtgtccggt ggaccgaccg tcggcgtgct
     5221 gatcgccgcg tacaacgagg cgccggtgct gccggcgacg ctgcgcgcgc tgctggccca
     5281 ggccgacccg ccggagcaga tcgtcgtggc cgacgacggg tccgacgacg gcaccgctgc
     5341 cgcgctggag gccgagttcg gtgaccggca ccacccgtcg ctgcaactgc tgaaacttgc
     5401 ccatggcggc aaggcggtcg ccctcaatgc ggcgcttatg gccgtcgaca ccgacgtcgt
     5461 cgtcacgatg gacgccgaca ccctgcccgc accggacgcc atcgccgcga tccggaacgc
     5521 gttcgcggcc gaccccgatc tcgtcgtggc gggcggcgtg ctgacaccga tgtgcggggc
     5581 gagccttcgc aatcggatac tgcagtggtt ccagacctat gaatacctgc gcaacttcgt
     5641 cgggcgctac gcgtggatgc gggtgaactg cctgctgctc atttcggggg cgttcgccgg
     5701 attccggcgc aacgccgtcc tctccgtcgg cggcttcgac accgactgcc tggtcgagga
     5761 ctacgagctc attcaccgcc tgcagcggcg cagccgcgat gacggcctgg gctggcgggt
     5821 gcagatcgtc ggcaacgccg tcgcgcagac cgacgcaccc ggcacgatcc gcgacttcct
     5881 gcgtcaacgg cgccgctggt tcggcggctt cctgcagacg cagtactgga accgggacat
     5941 gaccggcaac ccccgctacg gcgcactcgg gttggcgatg ctgccggtca aggccgtcga
     6001 cacggtgcag ccgatctacg ggctgaccgc gttcgtcgtg ctgatcgtgc tggcggtcac
     6061 cggcaacgcc gccgtcctga ttccggtggg cgcgattgtg ttgaccaagc tcctggtgga
     6121 cctcttcgcc tatgtgtgga cgattcacct ctaccgacgc tggaccggct ctgacgccaa
     6181 cacgcatttc ggcgccgggc tgctggccgc gttcgtcgag ccgttcacgt tcattccgct
     6241 gttgcacacc tgtgcgctgc tgggctggtg gcagttcctc accggccgcc agacgtgggt
     6301 tcctcagcgc cgcagaggaa tagtcgcctg acggccgagc gggtgtcatt ggcgtgaaca
     6361 ccgacgccgt aggggttcat ggtgtcagta ccaaccacga cggtgtgcag ggtgaaacca
     6421 cgcatcctga tcgggcgggt gtgaccggaa ccaacctgtc cggtaacggc gtcttcgggc
     6481 agagccaagc ggtcaacgcg ttcggcgtgt gtggcatcaa caccgcaggg gttggcgttc
     6541 gcggcgacag cgaggaccac gacggcgtgc agggatttgc aaacaacacc gataaggccg
     6601 gggtggtcgg cactcacctg ggttcggggg tcggtgtgcg gggccagtcg gtgttcggta
     6661 ccggcgtgca cgcggccagc gaaaacagca tcgggttgga ggccagcggc tcgatcgccg
     6721 cgcggctggt cggcaaaccg tcgtcgaagc gacctcgacg tcaccggtga cgtgttcctg
     6781 tctggtgccg actatgcgga ggcgctcagc acgaccgacg agtcggtgcg agaaggcgtc
     6841 gtcgtagtcc tcggccatga cggccaagtg catccgtgtc aacaggatta cgacaccgct
     6901 gtggcaggca ttgtgtccgg cgccggaggc tacaaacccg caattgtgct cgaccgccat
     6961 gagaacagcg ctcacgtcgc attgatggga aaggtgtggt gcctggcgga cgccgacatc
     7021 gcaccgatcc ggccgggcga cctgctgaca acgtcgagca cgacggggca ctgccggcgg
     7081 gtgacggaca catctagggc gttcggtgcg gtgatcggca aggcgctctc gccgcttccc
     7141 aacggtcgtg ggctgatccg cgtcctcgtc agcgcgaggt gaccggccgt gatccaggtt
     7201 cggcgggtgt tcggccatgg cgcgcggtct ttcgacgtca ctcgctcagt cctcaacttg
     7261 aaccgtccgg cgtcgatacg ccagatgaga gacccatacc tccaggacat ggtgttcgac
     7321 gagccgatca tcaccagcga cggggttgac ctcggcggtc atcaaaccct gaccgtgcga
     7381 aacgacggta ccttccgctg gcagggccac atgagggcga ccggattaca gtctttcgaa
     7441 ttcgcgatca tcaccgcgct ggtacatccg attgcgctgc cggacggtac gtcggcgacg
     7501 tcgcggatcg tgttcgcaga gagtggagag gttcacggct ccaacaagcc gggcgaccga
     7561 gtatttgcct gggaccaaca gagtgagaac aaactgctcc gttcggaatg gctcggggtg
     7621 cgtcaggggc gcatcgagca ccggctggag ttcgacaacg atcttttcgg ggtcgtgggc
     7681 gacgtggtga gctttctggc ccaggtcgtc gcgcttggcg ccaccttcgg cgccgctggc
     7741 gtagcgctgg tggtagctgg taaagcggcc gcgttgctca acgtcgacca gatcgtgttg
     7801 cccgggatga tcggcgtgct cttctctgcc ggcgcggcct tcgtgatcgg gccggtggca
     7861 atgtttcccg tgctcgttgt tggcgccgtc accgccgccg cactgataag gcagcgcgag
     7921 atgacccccg aggagcgcgc gttcgccgac caggtctttg cgggcaaaat caggtacgac
     7981 cgggtccgcc tgaccaatct cgtcggagtc ggcgagcggc ccttcaccgc gccgggtccc
     8041 ggcgagacga tcctggtcaa cctcggtaag ggattcgaca gtcccacaac gtataccgga
     8101 ctcggcggcg aggcgaccgg cattcgcgca ccgggacagc tgctgatcca cgagctg
//
LOCUS       DASWBF010000533        18695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1171869,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000533 DASWBF010000000
VERSION     DASWBF010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18695
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171869"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="VF874_19895"
     CDS             complement(<1..96)
                     /locus_tag="VF874_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S49"
                     /protein_id="HEX9178237.1"
                     /translation="MFSFLPPVPGLDDLRSLGRKVDTARHHGVPNG"
     gene            complement(106..792)
                     /locus_tag="VF874_19900"
     CDS             complement(106..792)
                     /locus_tag="VF874_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9178238.1"
                     /translation="MTDIPTVDEALARLNMPLLDAMMTQRAVRRVLPDPVDDAIVLKC
                     IELALRAPTGSNGQNWEFVVVKDRGVKEQLARRYRQAWSIYGAIGRRVAAGDESMLKV
                     LRAVQWQVDHFSEIPMLVVPCLRGGMRLPYVPSPFVGESSFFGSIYPSVQNLLLAARA
                     MGLGASLITLPLWSVTSARRTLGLPLTVTPCCVVPLGWPRGRYGPTTRKPVEEVAHLD
                     GYGNRAWLNE"
     gene            complement(881..1321)
                     /gene="rplO"
                     /locus_tag="VF874_19905"
     CDS             complement(881..1321)
                     /gene="rplO"
                     /locus_tag="VF874_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX9178239.1"
                     /translation="MTIKLHDLKPAPGSKTKRTRVGRGEGSKGKTAGRGTKGTKARKN
                     VPATFEGGQMPIHMRLPKLKGFRNRFRIEYAVVNVGDINRLFPEGGDVGVDELVAKGA
                     VRKNTLVKVLGDGKLAVKVSVSAHKFSSSAREKITAAGGSVTEL"
     gene            complement(1321..1500)
                     /gene="rpmD"
                     /locus_tag="VF874_19910"
     CDS             complement(1321..1500)
                     /gene="rpmD"
                     /locus_tag="VF874_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX9178240.1"
                     /translation="MADLRITQVRSTIGARWKQRESLRTVGLRRIRHSVVREDTPQTR
                     GLIAVVRHLVEVEEA"
     gene            complement(1503..2174)
                     /gene="rpsE"
                     /locus_tag="VF874_19915"
     CDS             complement(1503..2174)
                     /gene="rpsE"
                     /locus_tag="VF874_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX9178241.1"
                     /translation="MAEQAAGAAGPATSEGRPSRGDREGRGRRDDRGGRGRDSGEKSN
                     YLERVVAINRVSKVVKGGRRFSFTALVIVGDGNGMVGVGYGKAKEVPAAIAKGVEEAR
                     KTFFRVPLIGSTITHPVQGEAAAGVVMLRPASPGTGVIAGGAARAVLECAGVHDILAK
                     SLGSDNAINVVHATVAALKLLQRPEEVAARRGLPIEDVAPAGMLRARRESAALAAAAT
                     REGPA"
     gene            complement(2200..2604)
                     /gene="rplR"
                     /locus_tag="VF874_19920"
     CDS             complement(2200..2604)
                     /gene="rplR"
                     /locus_tag="VF874_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX9178242.1"
                     /translation="MATTQDNAKQKPVGQSVAAVRRTARLRRHARLRKKVSGTPERPR
                     LVVNRSSRHIHVQLVNDLNGTTLAAASSIEPDVRGVEGDKKAHSVRVGQLIAERAKAA
                     GIDTVVFDRGGYTYGGRIAALADAARESGLKF"
     gene            complement(2607..3146)
                     /gene="rplF"
                     /locus_tag="VF874_19925"
     CDS             complement(2607..3146)
                     /gene="rplF"
                     /locus_tag="VF874_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX9178243.1"
                     /translation="MSRIGKQPIPVPSGVDVTIDGQNVSVKGPKGELALQVAEPITVA
                     RDDDGAIVVTRPDDDRRNRSLHGLSRTLVSNLVTGVTRGYTTKMEIFGVGYRVQLRGA
                     NLEFALGYSHPVVIEAPEGITFTVESPTKFSVSGIDKQKVGQISANIRRLRRPDPYKG
                     KGVRYEGEQIRRKVGKTGK"
     gene            complement(3162..3560)
                     /gene="rpsH"
                     /locus_tag="VF874_19930"
     CDS             complement(3162..3560)
                     /gene="rpsH"
                     /locus_tag="VF874_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX9178244.1"
                     /translation="MTMTDPIADFLTRLRNANSAYHDEVTLPHSKIKANIAEILKREG
                     YIADYHTEDARVGKSLVVQLKYGPSRERSIAGLRRVSKPGLRVYAKSTNLPRVLGGLG
                     VAIISTSSGLRTDRQAAREGVGGEVLAYVW"
     gene            complement(3580..3765)
                     /locus_tag="VF874_19935"
     CDS             complement(3580..3765)
                     /locus_tag="VF874_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX9178245.1"
                     /translation="MAKKALVNKQQRKPKFKVRGYTRCNRCGRPHAVYRKFGLCRICL
                     RELAHAGQLPGVQKSSW"
     gene            complement(3765..4358)
                     /gene="rplE"
                     /locus_tag="VF874_19940"
     CDS             complement(3765..4358)
                     /gene="rplE"
                     /locus_tag="VF874_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX9178246.1"
                     /translation="MTTDTHPGASLAQEEKIQPRLKTRYREEIKDSLFKQFGYANVMQ
                     IPGVVKVVVNMGVGDAARDAKLINGAVNDLALITGQKPEVRRARKSIAQFKLREGMPI
                     GARVTLRGDRMWEFLDRLITIALPRIRDFRGLSPKQFDGTGNYTFGLTEQSVFHEIDV
                     DSIDRPRGMDITVVTSATTDDEGRALLRQLGFPFKEN"
     gene            complement(4358..4675)
                     /gene="rplX"
                     /locus_tag="VF874_19945"
     CDS             complement(4358..4675)
                     /gene="rplX"
                     /locus_tag="VF874_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX9178247.1"
                     /translation="MKIHKGDTVLVISGKDKGAKGKVLKAYPTANKVLVEGVNRIKKH
                     TPISTNQRGARSGGIVTQEAPIPVSNVMIVDSDGKPTRVGYRVDDETGKKVRISRRNG
                     KDI"
     gene            complement(4676..5044)
                     /gene="rplN"
                     /locus_tag="VF874_19950"
     CDS             complement(4676..5044)
                     /gene="rplN"
                     /locus_tag="VF874_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX9178248.1"
                     /translation="MIQQESRLKVADNTGAKEILCIRVLGGSSRRYAGIGDIIVATVK
                     EAIPGGNVKRGEIVKAVVVRTAKERRRPDGSYIKFDENAAVIIKPDNDPRGTRIFGPV
                     GRELREKRFMKIVSLAPEVL"
     gene            complement(5380..6543)
                     /locus_tag="VF874_19955"
     CDS             complement(5380..6543)
                     /locus_tag="VF874_19955"
                     /inference="COORDINATES: protein motif:HMM:NF023223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetD
                     domain-containing protein"
                     /protein_id="HEX9178249.1"
                     /translation="MTEWSTPASVRTRLRKRWSTGAALTAHARGEAFVPLDLPLRGPT
                     ASQVGARYADVAAWVRDWAHRPAGCTLTTKVIGARRTGGSEIPDRLRIESFDGLTAFL
                     GTADQARRHRELVEDAAAVAPQIRAWVVDRPMRALDHHGVFDRLLACVRWLLANADRD
                     RYFRQIDVPSVDTKFIERHRAVLAELLDSVIAPVDQQTRDFAERYGFRSKPPRVRIRI
                     LDPRSSPLPAGITDVELPVDELRGHRFDVERVFVVENEVTCLAFPTVPRSLLIFGGGY
                     AVSRVSRLDWLRDVALYYWGDIDTHGFRILDLMRKGFRHTRSVLMDRTTLLAHEDHWD
                     REPAPVNADLTNLTDNEAALYRDLVEDVFGTAVRLEQERISYSVVEAAVNDMR"
     gene            complement(6540..9938)
                     /locus_tag="VF874_19960"
     CDS             complement(6540..9938)
                     /locus_tag="VF874_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012833465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9178250.1"
                     /translation="MTALFPPTDLDEPDAATGFRLQRLEVFNWGTFDRRVWRLELGGR
                     NCLLTGDIGSGKSTLVDAVTTLLLPAHRIAYNKAAGAQNRERDLRSYVLGFYKSERNE
                     ATGRSRPVGLRGAGTYSVILGHFRNTGFDTDVTLAQVFWTKDGIGGQPERFYVTADAE
                     LSIGEHFSDFGAELPALRKRLRRIGAAVYDHFPDYSRDYRRKLAIASEQAMDLFHQTV
                     SMKSVGELNDFVREHMLEPFDAAGWVDKLVAHFEDLTQAHDAVVKARNQLAALGPLVE
                     DCDTHDRLQARLADLHGQRDALRYYFAALRQRLHTERATALDLELAEAQRRLEHTEAT
                     LASLRGELRSLQLEQAGHGGAEIADIDRRIEDCSAHRDQRRARRQRYDDYLAKAGLEQ
                     VSDAAQFAARSDGIATAAVNLDTELADLANRITEDAVTKRALEAEAKEIATELASLRQ
                     RRSNIPERNMSIRERLCAELRLQPSEIPFAGELIQVAAQHAEWVGAAERVLRGFGLSL
                     LVPQALYPRVSDWVDVNHLGGKLVYYRVPATVAVQDTIAVPAGALAHKLDVKDGPFSA
                     WVERELRRRASHICVDTMDQFRRHDYALTRAGQVKARGGRHEKDDSHPIGDRRHYVLG
                     WSNEQKIDALLTDAQRVQAELHRVGEALARLTVDREAVSARKSAVDTLSGVAASFDDI
                     DWESTVSEIARLQQRKRELSSASGELARIGAQIERTEAAIDDADRNRKDIDQRIGMLT
                     HDRDRARHGAAEAAQVAAEPDATEASRHFDAVTARLVDPPATADACIGEEHRHYEEIT
                     REIERVGGKSDTLARRIMTRMADFRRSYPADTVEMDDNVASAGEYRALRARMVDDDLP
                     RFEAAFKEYLNTNTIRDIAGFHSELNKQVELISSRIATINESLVGIDYNEGRYIRLEE
                     SPTPNQEIRAFRDELRQCADGSIGSDDTDQYSEDRFLLVKNLIEKFRGREGQTESDRN
                     WVKRVTDVRNWCTFAASERWRADDTEHENYTDSGGKSGGQKEKLAYTILAASLAYQFN
                     LNWGSKRARDFRFVVIDEAFGRGSDESTRFALTLFRKLGLQLLIVTPLQKIHVIEPFV
                     ASVGYVENRNGNASQLQCLTIEEYRRRKLLHSMIALDDTDAAVS"
     gene            complement(9935..10540)
                     /locus_tag="VF874_19965"
     CDS             complement(9935..10540)
                     /locus_tag="VF874_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008380238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HEX9178251.1"
                     /translation="MSANRVVSRAELNLPLVITHLMKGVVYRDTHDPVWQHLVQLVPQ
                     VTDYVSVMALVVVVDESEGYAFLRSKPEEPEDDSPPIPRLIARHALSFHTSLMLALLR
                     KKLAEFDATDDGFRLIVGRDQIVDMMLMFMATSTNEARIVDTVERTIGKVVDLGFLRR
                     LPKQDDQFEVRRVLKAFVDGQWLSEFDARLADYAAELEESR"
     gene            complement(10537..11994)
                     /locus_tag="VF874_19970"
     CDS             complement(10537..11994)
                     /locus_tag="VF874_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009677670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HEX9178252.1"
                     /translation="MRHEEIAALRAGSPAWRLLRADNAPLVLSFLGQVFVDDNVRDIS
                     ASQLISRLDDELFALNERLGEGTYPKSAKAYLDDWSAPEAGWLRKFYPLGSDEPHYDA
                     TAAVERAVAWVRGLRDRAFVGTESRLNTVFELLRQLVFGSETDGQTRLAELHRRRAEL
                     DREIAAVESGDFAVLDPTARRDRYQQFADTARALLADFREVEANFRRLDRELRERITT
                     WDGAKGQLLDEVVGDRSHIADSDQGRSFRAFYDLLLSPTRQRELTELLHRVHQLDLAE
                     HADPRLRYIHHDWLDAGERAQATVRQLSEQLRRFLDDQVWLENRRVMELLRSIEARSL
                     AFRDRIGADFAYEIDATAPTIRLPLERPLYTRPRAATVESATVEQGRFDSESVALFEQ
                     TYVDPAPLIRSVCRALRRAPQVGLAEVVSETPITCGLSELVTYLSLDDERFTTVFDES
                     ARETVGWTDDTRTTRTAVLPRVLYVRKLSDESART"
     gene            complement(12097..12513)
                     /locus_tag="VF874_19975"
     CDS             complement(12097..12513)
                     /locus_tag="VF874_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178253.1"
                     /translation="MTRNTPPKTRRVRNVVAGGTSIAAGALLLVTGVLTLLQGISAVV
                     NDELLVVTADYVYKFNTTVWGWIHIILGVLLAAIAIGLIRGTDWARVAAIVIASVSIV
                     VMFLWLPYYPVWSLVVIALDVIVIWAVTTWDNGRAR"
     gene            complement(12552..14246)
                     /locus_tag="VF874_19980"
     CDS             complement(12552..14246)
                     /locus_tag="VF874_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9178254.1"
                     /translation="MTASQSVSLGGTEPYFDLGSYHRPIETPSPQAQIWFDRGLVWAY
                     AFNHEEAVRCFERALELDPALAAARWGIAYSVGPNYNKAWEAFDPVDLAASLARARME
                     LQLAAGGRASAVERGLIEALAARFPTDDPDDADALQAGLVGYAEAMGELARAYPDDVD
                     VAALAADALVNVTAWALWDVRTGEPAPDSRVVEAKRILEDALGSAEGRRHPGILHLYL
                     HTMEMSATPEDALPAADLLRGLVPDAGHLQHMPSHIDVLCGDYRNSVVSNQAAVQADR
                     RFVERAGPLNFYSLYRAHDLHFIVYSAMFEGQSQIGLQAADELASQLTPELLSIESPP
                     MADWLEAFVPLRIHVLVRFGRWDDLIAEPIPADVDLYCTTAATIHYGRGVAHAAKGQL
                     PQARAERASFAEAYARIPESRYLFNNTSRDILAIAEAMFDGEIAYREGSFDTAFGHLR
                     RAIELDDSLPYDEPWGWMQPTRHAYGALLLEQGHVEEAAAVYAADLGLDPTLTRACQH
                     PRNVWSLHGYHECLQRLGRTEEAVIIRQQLALARARADVPVLASCACRLEVACCDD"
     gene            complement(14268..15155)
                     /locus_tag="VF874_19985"
     CDS             complement(14268..15155)
                     /locus_tag="VF874_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX9178255.1"
                     /translation="MLTELVELPGGSFRMGSTSFYPEEAPVHTATVAGFAIERHPVTN
                     AQFAEFMSATGYVTVAEQPLDPALYPGAATADLVPGAMVFRPSSGPVDLRDWRQWWDW
                     APGVSWRHPFGPGSSVEDKPDHPVVQVAYPDAWAYAKWAGRRLPTEAEWEYVARAGCS
                     SVYAWGDEPTPGGHLMANTWQGRFPYRNDGADGWVGTSPVGTFPANGFGLLDMIGNVW
                     EWTTTEYSTHHRVGQPCCGPSVSSADPTVSQTLKGGSHLCAPEYCHRYRPAARSPQSR
                     DTATTHIGFRCVADLGSSG"
     gene            complement(15168..17519)
                     /locus_tag="VF874_19990"
     CDS             complement(15168..17519)
                     /locus_tag="VF874_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX9178256.1"
                     /translation="MASEFAGKIELDIRNSEPDWGPYAAPTAPDGAPNVLYVVWDDTG
                     IATWDCFGGLVEMPTMSRIAERGVRLSQFHTTALCSPTRASLLTGRNATTVGMATIEE
                     FTDGFPNCNGRIPFDTALISEVLAEQGWNTYCVGKWHLTPLEESNLAATKRHWPLARG
                     FERFYGFMGGETDQWYPDLVYDNHPVTPPATPEDGYHLSKDIADKTIEFIRDAKMIAP
                     DKPWFSYVCPGAGHAPHHVFKEWADKYAGKFDIGYERYREIALENQKKLGIVPPDTEL
                     SPVNPYLDVKGPNGEPWPLQDTVRPWDTLGDEEKKLFCRMAEVFAGFLSYTDAQLGRI
                     LDYLDESGQLDNTVIVVISDNGASGEGGPNGSVNEVKFFNGYIDTAEESMRFYDNLGG
                     TDTYNHYPIGWAMAFNTPYKLFKRYASHEGGIADTAIISWPNGIAAHGEVRDNYVNVC
                     DITPTVYDLLGITPPDTVKGIAQKALDGMSFKAALDDPKADTGKDTQFYEMLGTRGIW
                     HKGWFANTVHAATPSGWSHFDKDRWELFHIEADRSQCHDLAEKESSKLVELTKLWFDE
                     AAKYNGLPLADLNLLETLTRWRPYLAGDRKSFVYYPNTAEVGIGAAVEIRGQSFSVLA
                     EVTVDTTGAEGVIFKQGGAHGGHVLFVQDGRLHYIYNFMGEDEQKVSSSGAVPLGKHI
                     FGVRYTLQGTVEGSHTPLGEVTVYIDEQAVGTRPNVKAHPGTFGLAGASLSVGRNAGS
                     AVSSSYKAPYAFTGGTIQQVVVDVSGEPYQHVEKELALAFAKD"
     gene            complement(17752..>18695)
                     /locus_tag="VF874_19995"
     CDS             complement(17752..>18695)
                     /locus_tag="VF874_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEX9178257.1"
                     /translation="AQFSSQYGNAAILQQSGSTSAGLTGGLMFALICIGLLAPTLAPI
                     AAWTAVARWRWHTWQTVIVPAALYGAALSFQALSYASGSTFGFLRFYIVAVPFAATLA
                     LLAVPDGVVSPATRRGKYALASPEPVPVSGRWRFAYAVVLLAFAVCVPVTAWGMGQPK
                     YAPQEYALGAVLAPQPDNVTERKAIEHRIAASFSTERDIAGYLGRLNLPESSVLTDTV
                     YGFAIVAASERPRTFIVPSDPDFTALLNDPVTGGIEYLLAVPPTGRGASDALNLRYPT
                     LYDTGADIATLELEVPNNGDSQPTWRLYRVNKPVPAS"
BASE COUNT         3014 a         6249 c         6102 g         3330 t
ORIGIN      
        1 cccgttcggt acgccgtgat gacgggcggt gtccaccttg cggcccagtg accgcaggtc
       61 gtcgaggccc ggaacgggcg gcaggaaact gaacatgccc caagcctatt cgttgagcca
      121 tgcgcggttg ccgtacccgt cgaggtgagc cacctcctcg accggtttgc gggtcgtcgg
      181 cccgtagcgc ccacgcggcc aaccgagggg gacgacgcag cacggggtga ctgtgagcgg
      241 cagaccgagg gttctgcggg ccgacgtcac gctccacagc ggcagggtga tcagcgatgc
      301 gccaagcccc atcgcgcgcg ccgccagcag caggttctgc acgctcgggt agattgatcc
      361 gaagaatgac gactcgccca cgaaaggcga tggcacgtac ggcagccgca tgcctccgcg
      421 caggcagggc acgacgagca ttgggatctc gctgaagtgg tcgacctgcc attgcactgc
      481 acgcaggact ttcagcatcg actcgtcgcc ggccgccaca cgccggccga tggcgccgta
      541 gatcgaccag gcttggcggt agcgcctggc caactgctcc ttcacgccgc ggtccttcac
      601 gacgacgaac tcccagttct gcccgttcga cccggtcggc gcacgcagcg ccaattcgat
      661 gcacttcagc acgatcgcgt cgtcaaccgg atccggtagc acgcgccgta cggctcgctg
      721 ggtcatcatc gcgtcgagca gcggcatgtt caggcgggca agcgcctcgt cgacggtcgg
      781 tatgtcagtc ataaccaccg acgctaccgc tgtgccatcg aaatctgcac tatggttgtg
      841 attcggcgaa aatcacagcc ctggtgcaga actcgcggcg tcagagttcg gtgacggatc
      901 cgcctgcggc ggtgatcttt tcgcgtgcgc tgctgctgaa cttgtgcgcg ctcaccgaaa
      961 ccttgaccgc gagcttgccg tcgccgagca ccttgacgag ggtgtttttg cgcaccgccc
     1021 ccttggccac caattcgtcg acgccgacgt cgccgccctc ggggaacagt cggttgatat
     1081 cgccgacatt gacgacggcg tactcgatgc ggaaccggtt gcggaagccc ttcagcttag
     1141 gcagccgcat gtggatcggc atctggccgc cctcgaaggt cgccggcacg ttcttgcggg
     1201 ccttggtgcc cttggtgccg cggccggcgg tcttgccttt cgagccttca ccgcgaccga
     1261 cgcgggtccg cttggtcttc gaccccggcg cgggtttcag gtcgtgaagc ttgatggtca
     1321 ctaggcctcc tcaacctcta cgagatggcg caccaccgcg atcagtccgc gcgtctgcgg
     1381 ggtgtcctca cgaaccaccg agtgccgaat ccgcctcagc cccaccgtcc gcaagctctc
     1441 ccgctgcttc catcgcgctc cgatggtgct gcgcacctga gtaattcgaa gatcggccat
     1501 ggctatgccg gtccttcccg cgtggccgca gccgccagcg cggcgctctc gcggcgcgcc
     1561 cgcagcattc cggccggggc gacgtcctcg atcggcaggc cgcggcgggc cgccacttcc
     1621 tccggtcgct gcagcagctt caacgcggcc accgtggcgt gcaccacgtt gatcgcgttg
     1681 tcgctaccca gcgacttggc cagaatgtcg tgcacgccag cgcattccag caccgcgcgt
     1741 gcggcgccgc cggcgatcac accggtaccg gggctggccg gacgcagcat caccacgccc
     1801 gcggcggcct ccccctggac ggggtgcgtg atcgtgctgc cgatcagcgg tacccggaag
     1861 aaggtcttgc gcgcttcctc cacacccttg gcgatcgcgg caggcacctc cttggccttg
     1921 ccgtacccga caccgaccat cccgttgccg tcgccgacga tcaccagcgc ggtgaagctg
     1981 aaccggcggc cgcccttgac caccttcgac acgcggttga tcgcgacgac gcgctcgagg
     2041 tagttgctct tctccccgct gtcacgcccg cggccgcccc tgtcgtcacg acgaccgcga
     2101 ccctcgcggt caccccgcga cggccggcct tcggatgtcg ccggcccggc cgctcctgct
     2161 gcctgctcgg ccatcatggt gtccttccgt tgtaagtcat cagaacttca gtccgctctc
     2221 tcgcgccgcg tcggccagtg ccgcgatccg accgccgtag gtgtagccac cccggtcgaa
     2281 caccacggtg tcgatgccgg cggccttggc ccgttcggcg atcaattggc cgacccggac
     2341 gctgtgggct ttcttgtcgc cttcgacccc gcgcacgtcg ggttcgatcg acgacgcggc
     2401 cgccagcgtg gtcccgttga ggtcgttgac cagctgcacg tggatgtgcc gagacgaccg
     2461 gttgaccacc agccgcggcc gctccggcgt gccggacacc ttcttgcgca gccgggcgtg
     2521 ccgacgcagc cgcgcggtgc ggcgaaccgc ggcgacgctc tgcccgaccg gcttctgctt
     2581 cgcattgtct tgagtggtag ccatttctac ttgcccgtct ttccgacctt gcggcgaatc
     2641 tgttcaccct cgtagcgcac gcccttgccc ttgtacgggt cgggacgacg caaccggcgg
     2701 atgttcgccg agatctggcc gaccttctgc ttgtcgatgc ccgagaccga gaacttcgtc
     2761 gggctttcga ccgtgaacgt gatgccctcg ggcgcctcga tcaccaccgg atggctgtag
     2821 ccgagcgcga attcgaggtt ggcgccccgc agctgcacgc ggtagccgac gccgaaaatc
     2881 tccatcttgg tggtgtagcc ccgcgtcaca ccggtgacca ggttgctcac cagcgtgcgc
     2941 gacagcccgt gcagcgagcg gttgcgccga tcgtcgtcgg gccgggtgac cacgatcgcg
     3001 ccgtcgtcgt cgcgggccac cgtgatcggc tcggcgacct gcaaagccaa ttcgcccttg
     3061 gggcccttga cggagacgtt ctgaccgtcg atcgtcacgt cgacacccga cggaacgggg
     3121 atcggctgct taccaatgcg cgacatgttt ctcgttcctc ctcaccacac gtacgcgagg
     3181 acttcgccgc cgacgccctc gcgggccgcc tggcggtcgg tcctcagacc cgacgaggtg
     3241 gagatgatgg ccacgccaag gccgccgagc actcgcggca ggttggtcga cttcgcgtac
     3301 accctcaggc ccggcttgga cacccggcgc aggccggcga tgctgcgctc ccggctcggc
     3361 ccgtacttca gttgcaccac aagggatttg cccacgcggg catcctcggt gtggtagtcg
     3421 gcgatgtagc cctcgcgctt gaggatctcg gcgatgttgg ccttgatctt gctgtgcggc
     3481 agggtcactt cgtcgtggta cgccgaattg gcgttgcgca gacgtgtcaa gaagtctgcg
     3541 atcgggtcgg tcatcgtcat ggcatgtcct cgctaatgct caccagctgc tcttctgtac
     3601 gcccggcaac tggccggcat gcgccaactc acgcaggcag attcggcaca ggccgaactt
     3661 gcggtacacg gcgtgcggac gtccgcagcg gttgcaccgc gtgtagccgc gcaccttgaa
     3721 cttcggtttg cgctgctgct tgttgactag agccttcttc gccatcagtt ctccttgaac
     3781 ggaaagccga gttgccgcaa cagcgctcgc ccttcgtcgt cggtcgtcgc cgaggtgacg
     3841 acggtgatgt ccatgccccg cggccggtcg atgctgtcca cgtcgatctc gtggaacacc
     3901 gactgctcgg tcagcccgaa cgtgtagttg ccggtgccgt cgaactgctt gggcgacagc
     3961 ccgcggaagt cgcggatacg cggcagcgcg atcgtgatca gccggtcgag gaattcccac
     4021 atccggtcac cgcgcagcgt cacccgcgcg ccgatcggca tgccctcgcg aagcttgaac
     4081 tgcgcgatcg acttacgagc gcgccggacc tccggcttct gcccggtgat cagcgccagg
     4141 tcgttgaccg cgccgttgat cagcttcgcg tcgcgggcgg cgtcgccgac gcccatgttc
     4201 accacgacct tcacgacgcc cgggatctgc atcacgttcg cgtagccgaa ctgcttgaaa
     4261 agcgaatcct tgatctcttc gcggtagcgg gtcttcagcc tgggctggat cttttcttcc
     4321 tgggcaagcg aagcgccggg atgggtatca gtggtcatca gatgtccttg ccgttgcggc
     4381 gcgagatgcg gaccttcttg ccggtttcgt cgtcgacgcg gtagccgacg cgggtgggtt
     4441 tgccgtcgga gtcgacgatc atcacgttgc tgacggggat cggcgcttct tgggtgacga
     4501 tgccgcccga gcgcgcaccg cgttggtttg tcgagatcgg ggtgtgcttc ttgatccggt
     4561 tgacgccctc cacgagcacc ttgttggcgg tcgggtaggc cttgagcacc ttgcccttgg
     4621 cgcccttgtc cttaccggag atgacgagca cggtgtcgcc tttgtggatc ttcatctaca
     4681 gcacctccgg ggccagcgac acgatcttca tgaaccgctt ctcgcgcagc tctcgcccga
     4741 cggggccgaa gatgcgcgtg ccacgcggat cgttgtcggg cttgatgatc acggccgcgt
     4801 tctcgtcgaa cttgatgtag ctgccgtccg gacgcctgcg ttccttggcg gtgcgcacga
     4861 ccacggcctt cacaatctcg ccgcgcttga cgttgccgcc cgggatggcc tccttgaccg
     4921 tcgcgacgat gatgtcgccg atgccggcgt agcgccgcga cgacccgccg agcacccgga
     4981 tgcacaagat ctccttggca ccggtgttgt cggcgacctt gagccgcgat tcctgctgaa
     5041 tcacaagatc tcctcgacag taatgtgcac gccgagggac agcctccacg tgcgcggtct
     5101 tgctgtttcc gaagggcacg cggggccaac cacccgatga gcgcttgcgc gaagagcatc
     5161 atgcggagca ggccagccga ggtctgccca aggcaacccc tagattctag gggaggacct
     5221 gcgaggagcc aaatccctca cctctcggcg cgagcgtgcg tgtttgttcc ccggcacgcc
     5281 ggtgtcggac ctgcaaaccc gcacgttcgc gctgtcgcga gcattagctg tcgcagcagc
     5341 cgacacgccc ctgaactttc tgcaaaactg caggcccagc taacgcatat cgttgaccgc
     5401 agcttcgacg accgagtagc tgatccgctc ctgctccagg cgcacggccg tcccgaagac
     5461 atcctccacc aggtcgcggt acagcgcggc ttcgttgtcg gtgagattgg tcaggtcggc
     5521 gttaacgggt gccggttcgc ggtcccaatg gtcctcgtga gccagcaacg tggtccggtc
     5581 cattagcacc gagcgcgtgt ggcggaaacc cttccgcatg agatcgagga tgcgaaaccc
     5641 gtgggtatcg atgtcgcccc agtagtacag cgcgacgtcg cgaagccagt cgagacggct
     5701 gacccgcgac actgcgtacc cgccgccgaa gatcagcagt gaccgcggca cggtcgggaa
     5761 cgccaaacaa gtcacctcgt tttccaccac gaagacgcgc tcgacatcga accggtgtcc
     5821 ccgcaattcg tcgacgggca attcgacgtc ggtgatacca gccggcagtg gcgagctgcg
     5881 gggatcgagg atgcgaattc gcacccgcgg cggcttgctg cgaaacccat agcgttcggc
     5941 gaagtcgcgc gtctgctgat cgacgggcgc gatcaccgaa tcgaggagtt cggcgagcac
     6001 tgcgcggtgc cgctcgatga acttggtgtc cacgctaggc acgtcgatct gacggaaata
     6061 gcggtcccgg tcggcgttgg ccagcagcca ccgcacgcac gccagcaacc ggtcgaagac
     6121 accgtggtgg tccagggcgc gcattggcct atccaccacc caggcgcgga tttgcggcgc
     6181 cacagcagcg gcgtcctcga cgagttcgcg gtggcggcgc gcctgatccg ccgtgccgag
     6241 aaacgcggtg agaccgtcga acgactcgat acgcaggcgg tcggggattt cgctgccgcc
     6301 ggtacgacgc gcgccgatca ccttggtcgt caaggtgcag ccggccgggc ggtgcgccca
     6361 gtcgcggacc cacgccgcga cgtccgcgta gcgagcgccg acctgcgagg ccgtcggccc
     6421 gcggagcggc aggtcgagcg gcacgaaggc ctcacctcgc gcgtgggctg tcagcgcggc
     6481 gccggtgctc caccgcttgc gcagccgtgt gcgcacgctg gccggggtcg accattcggt
     6541 cacgacacgg cggcgtccgt gtcgtccagc gcgatcatgc tgtgcaacag cttgcgccgg
     6601 cggtattcct cgatggtcag gcattgcagc tgagaggcgt tgccgttgcg gttttccacg
     6661 tagccgaccg aggccacgaa cggctcgatg acgtgaatct tttgcagcgg ggtgacgatc
     6721 agcagttgca ggcccagctt gcggaacaag gtgagcgcaa accgagttga ctcgtcggag
     6781 ccccggccga acgcctcgtc gatgaccacg aagcgaaagt ccctggcacg cttcgaaccc
     6841 cagttgaggt tgaactggta ggccagcgac gccgccagga tggtgtaggc gagcttttcc
     6901 ttttggccgc cgctcttgcc gcccgaatcg gtgtagtttt cgtgttcggt gtcatcggcg
     6961 cgccaccgct cggacgcggc gaaggtgcac cagttgcgca cgtcggtgac cctcttgacc
     7021 cagttgcggt ccgactcggt ctggccttcg cggccgcgga acttctcgat aaggttcttc
     7081 actagaagaa agcggtcctc ggagtactgg tcagtgtcgt cggagccgat ggagccgtcg
     7141 gcgcactgcc gcagctcgtc tcggaaggcc ctgatctcct ggttaggtgt cggggattcc
     7201 tccaatcgga tgtagcggcc ctcgttgtag tcgatgccga ccagcgactc gttgatcgtg
     7261 gcgatacgcg acgagatcag ctcgacctgc ttgttgagtt cggagtggaa tccggcgatg
     7321 tcgcgaatgg tgttggtgtt caggtactcc ttgaatgcgg cctcgaaccg gggcaggtcg
     7381 tcgtcgacca tccgcgcgcg caatgcccta tactccccgg cggaggccac attgtcgtcc
     7441 atctccaccg tgtcagccgg atagcttcgc cgaaagtcgg ccattctggt catgattcgc
     7501 cgtgccagcg tgtccgattt cccgccgacg cgctcgatct cgcgggtgat ctcttcatag
     7561 tgccggtgct cctcgccgat acaggcgtcg gccgtcgccg gtggatcgac caggcgggcg
     7621 gttacggcgt cgaagtggcg cgacgcttcg gttgcgtcgg gttcggcagc gacctgtgcc
     7681 gcctccgcag cgccgtggcg ggcgcgatcg cggtcgtggg tgagcatgcc gatgcgctgg
     7741 tcgatgtcct tgcggttccg gtcggcatcg tcgatcgccg cttctgtccg ctcgatctgg
     7801 gcgccgatgc gggcgagttc tcctgaggcg gaactcaatt cgcgcttgcg ctgctgcaac
     7861 cgggcgatct cggaaaccgt cgattcccag tcgatgtcgt cgaacgatgc cgccacgcca
     7921 gacaacgtgt ccaccgccga cttgcgggcg ctgaccgctt cgcggtcgac cgtcagccgc
     7981 gccagcgctt cgccgacgcg gtgcagttcg gcctgcacgc gttgtgcgtc ggtgagcagc
     8041 gcgtcgatct tttgttcgtt gctccagccg agcacgtagt gacggcggtc gccgatgggg
     8101 tgcgaatcgt ctttctcgtg ccggccgccg cgggccttga cttggccggc ccgggtgagc
     8161 gcgtagtcgt ggcgacgaaa ctggtccatc gtgtcgacgc aaatatgcga tgcgcgcctg
     8221 cgcaattcgc gttcgaccca ggcggagaac gggccgtcct tgacgtctag cttgtgcgcc
     8281 agcgccccgg cgggaacggc gatggtgtcc tgcaccgcca cggtagccgg cacccggtag
     8341 tagacgagtt tgccgcccaa gtggttgacg tcgacccagt ccgacacccg cggatacaga
     8401 gcctgcggca ccagcagcga cagcccgaat ccgcgcagca cccgctcggc ggcgcccacc
     8461 cactcggcgt gctgggccgc cacctgaatc agctcaccgg cgaaggggat ttccgatggc
     8521 tgcaggcgca gctcggcgca gagccgctcg cggatgctca tgttgcgctc ggggatgttg
     8581 ctgcgacgct gacgcaggct cgccaactca gtggcgatct ccttggcttc ggcctccaac
     8641 gcccgtttgg tgacagcgtc ttcggtgatc cggtttgcca gatcggccag ctcagtatcc
     8701 aaattcactg ctgccgtggc gattccatcg gagcgggccg cgaactgagc ggcgtcggac
     8761 acctgctcca ggccagcttt cgccaggtag tcgtcgtagc gttggcgtcg cgcgcgacgc
     8821 tggtcacggt gcgccgagca gtcctcgatg cgccgatcga tgtcggcgat ctccgcgccc
     8881 ccgtggccgg cctgctccag ctgcaggctg cgcaactcgc cgcgcagcga tgccagcgtg
     8941 gcctcggtat gttccagacg ccgctgcgcc tcggccagct ctagatccag cgccgtcgcg
     9001 cgttcggtgt gcaggcgttg gcgtagcgcg gcgaaatagt agcgcagcgc gtcgcgctgc
     9061 ccgtgcagat cggcgagtcg agcctgcagt cggtcgtggg tgtcgcagtc ctcgacgagc
     9121 ggccccagag cggccagctg gttccgtgct ttgaccacgg cgtcatgcgc ttgagtcagg
     9181 tcttcgaaat gcgcgaccag cttgtcgacc cagccggcgg cgtcgaacgg ttccagcatg
     9241 tgctcgcgca cgaaatcgtt gagctcgccc accgacttca tcgacacggt ctggtggaac
     9301 aggtccatgg cctgttcgga ggcgatcgcc agcttgcgtc ggtagtcgcg gctgtagtcg
     9361 ggaaaatggt cgtacacggc tgcgccgatc cttcgcagcc gcttgcgcag agcgggcagt
     9421 tcggcgccga aatcgctgaa gtgctcgcct atgctcagtt cggcgtcggc tgtcacgtag
     9481 aaccgctccg gctgaccgcc gataccgtcc ttagtccaaa acacctgcgc cagcgttaca
     9541 tcggtatcga agccggtgtt gcggaagtga cctaggatca ccgagtatgt gccggcgcca
     9601 cgcagcccca ccggccgtga ccggccggtg gcctcgttgc gctcggactt gtagaagccg
     9661 agcacatacg accgcaggtc gcgttcccgg ttctgcgctc cggcggcctt gttatacgcg
     9721 atgcggtgtg cgggcagcag caacgtggtg acggcgtcga cgagagtcga cttgcccgag
     9781 ccgatatccc cggtcagcag gcagttgcgg ccgcccaatt cgagccgcca cactcgccgg
     9841 tcgaaggtgc cccagttgaa cacctccaaa cgctgcagcc gaaacccggt tgcggcgtcg
     9901 ggttcgtcga ggtcggtggg cgggaacagt gcggtcaccg cgactcctcc agttcggcgg
     9961 cgtaatcggc taagcgggcg tcgaactcag agagccattg cccatcgacg aaggccttca
    10021 gcactcggcg cacctcgaac tggtcgtcct gcttaggaag tcgacgcagg aagccgaggt
    10081 cgaccacctt tccgatggtg cgttcgacgg tgtcgacgat gcgggcctcg ttggttgacg
    10141 tggccatgaa catcagcatc atgtcgacga tctggtcgcg gccgacgatc agccggaagc
    10201 cgtcgtcggt ggcgtcgaac tcggccaact tttttcgcag caacgccaac atcaggctgg
    10261 tgtgaaacga cagcgcgtgg cgggcgatca gccgagggat cggcggcgaa tcgtcctcag
    10321 gctcctcggg tttggaacgc aggaatgcat acccctcgga ttcgtcgacc accacgacga
    10381 gtgccatcac ggaaacgtag tcggtcacct gcggcacaag ctgcaccaga tgttgccaga
    10441 ccgggtcgtg ggtgtcgcgg tacaccaccc ccttcatcag gtgggtgatc accagcggca
    10501 gattcaactc agctcggctg acgactctgt tggcgctcat gttctcgcac tttcgtcaga
    10561 tagcttgcgg acgtagagaa ctcgcggcag caccgcggtg cgggtggttc gggtgtcgtc
    10621 ggtccagccg acggtctcgc gggcgctctc gtcgaacacc gtcgtaaatc tctcgtcgtc
    10681 aagcgacagg tacgttacca actccgaaag gccgcaagtg atcggtgttt cggagacgac
    10741 ctcggcgagg ccgacctgcg gtgcccgtcg caacgcgcgg cacaccgacc ggatcagcgg
    10801 cgccggatcg acgtaggtct gttcgaacag cgccactgac tcgctgtcga atcggccctg
    10861 ctcgaccgtg gcactctcca ccgtcgcggc ccgcggcctg gtgtagagcg gccgctccag
    10921 tggcaaacgg atcgtcgggg ccgtggcgtc gatttcgtag gcgaagtcag caccgatgcg
    10981 gtcccgaaac gccagcgacc gagcctcgat gctgcgcagc aactccatga cgcgacggtt
    11041 ctccaaccac acctggtcgt cgaggaaccg gcgcagctgc tcggagagct ggcgtactgt
    11101 ggcctgcgcg cgttcaccgg cgtcgagcca atcgtggtgg atgtagcgca gtcgtggatc
    11161 agcgtgctcg gccaagtcga gttgatgcac ccggtgcagc agctcggtga gctcgcgctg
    11221 ccgggtcggg gacagcaaca gatcgtagaa cgcccggaag ctgcggccct ggtcggagtc
    11281 ggcgatgtgg ctgcggtcgc cgacgacctc gtcgagcagc tggcccttag cgccgtccca
    11341 cgtagtgatg cgctcccgca gttcgcgatc cagccggcgg aagttggcct ccacctcacg
    11401 gaagtcagca agcagggccc gcgccgtgtc ggcgaactgt tgatagcggt cccgccgcgc
    11461 cgtcggatca agtacggcga agtcaccgct ctcaacggcg gcgatttccc ggtccagctc
    11521 ggcgcgccgg cgatgcagtt cggccagccg cgtctgacca tcggtttccg agccgaacac
    11581 aagctgtcgc agcagttcaa acacggtgtt gagccgcgat tcggtgccga cgaacgcccg
    11641 atcgcgcaac ccccgcaccc aggccaccgc acgctccacg gccgccgtgg cgtcgtagtg
    11701 cggctcgtct gaaccgagcg ggtaaaactt gcgcagccag ccggcttccg gggcggacca
    11761 gtcgtcgagg taagccttcg ctgatttcgg ataggtgccc tctcccaggc gctcgttgag
    11821 tgcgaacagc tcgtcgtcga gccggctgat cagctgtgac gccgaaatgt ctcggacgtt
    11881 gtcgtcgacg aacacctgcc cgaggaagga caggaccagc ggtgcgttat cggcgcggag
    11941 cagccgccac gcaggactac ccgcccgtag tgccgcgatc tcttcgtgcc gcatgggcca
    12001 acggtaacgc tggccaccga catcccacgt cccgcgagcg cgcgtgtctg tccccgacac
    12061 gcccgcgaaa cgccggcaac cgcgcacgct cgccggctac cgcgcgcggc cgttgtccca
    12121 ggtcgtgacg gcccagataa cgatcacgtc cagcgcgatc accaccaacg accacaccgg
    12181 gtagtacggc agccacagga acatcaccac aatcgacacc gaagcgatga cgatcgccgc
    12241 cacccgggcc cagtccgtgc ctcgaatgag gccgatggcg atcgccgcca gcagcacgcc
    12301 caggatgatg tggatccagc cccacaccgt ggtgttgaac ttgtacacgt agtccgccgt
    12361 gacgacgagc agctcgtcgt tgaccaccgc agagatgccc tgaagcagcg tcagcacgcc
    12421 ggtcaccaac agcaaggcgc cggcggctat cgacgtgccg ccggcgacta cgtttcgcac
    12481 gcggcgggtt ttgggtgggg tgttgcgggt catgaattcc tcctttgcca gtgagcgcta
    12541 tcgccaaacg atcagtcgtc gcagcaggcg acctccagcc ggcaggcgca cgaagcgagc
    12601 accggcacgt cggcgcgcgc acgggccaac gccagctgct ggcggatgat caccgcctcc
    12661 tcggtgcggc ccagccgctg caggcattcg tggtaaccat gcaggctcca gacgttgcgg
    12721 gggtgttggc aggcgcgggt cagcgtcggg tcgaggccaa gatcggcggc gtagacggcg
    12781 gccgcctctt cgacgtggcc ctgctcgagc agcagcgcgc cgtaggcgtg ccgggtgggc
    12841 tgcatccagc cccatggctc gtcgtagggc agcgagtcat ccaactcgat cgcgcgccga
    12901 aggtggccga acgcggtgtc gaaagagcct tcacggtaag cgatttcacc gtcgaacatc
    12961 gcctcggcga tcgcaagaat gtcgcgactc gtgttgttga acaagtaccg tgattcaggt
    13021 atccgcgcat acgcctcggc gaacgaggca cgttcggcac gagcctgcgg cagttggcct
    13081 ttggcggcat gcgcgacgcc tcgcccgtag tgaatggtcg ctgcggtggt gcagtacagg
    13141 tcgacgtcgg cggggatcgg ctcggcgatc aggtcgtccc accggccgaa ccgcaccagc
    13201 acgtgaattc gcaacggcac gaacgcttcc agccagtccg ccatcggcgg cgactcgatc
    13261 gacagcagct ccggcgtcaa ctggctggcg agttcgtcgg ccgcctgcag cccgatctgc
    13321 gactgcccct cgaacatcgc cgaatacacg atgaagtgca gatcatgcgc acggtacagc
    13381 gagtagaagt tcaacggccc ggcacgttcg acgaaccggc ggtcggcctg caccgccgcc
    13441 tgattcgaca ccacggagtt gcggtagtcg ccgcacaaca cgtcgatgtg gctgggcatg
    13501 tgttgcaggt gtccggcgtc gggcaccagg ccgcgcagca ggtccgccgc cggcagcgcg
    13561 tcttccgggg tcgccgacat ctccatcgtg tgcaggtaca ggtgcaggat tccggggtgt
    13621 cgacgaccct cagcggaccc cagtgcgtcc tcgagaattc gtttcgcttc gacgacgcgg
    13681 gaatcgggcg cgggttcgcc ggtccggacg tcccacagcg cccacgcggt gacgttgacc
    13741 agcgcgtcgg ccgccaacgc ggccacgtcg acgtcgtcgg ggtaggcccg cgccagctca
    13801 cccatcgcct cggcgtagcc aacgagccca gcctgcaacg cgtcggcgtc gtccgggtcg
    13861 tcggtgggaa agcgggccgc caacgcctcg atcaggccac gctcgaccgc gctggcccgg
    13921 ccacccgcgg ccagctgcag ctccatccgc gcccgcgcca gcgacgcggc gagatccacc
    13981 gggtcgaaag cctcccacgc cttgttgtag ttcgggccga ccgagtaggc gatgccccat
    14041 cgcgccgcag cgagcgcggg gtcgagttcc agggcacgtt cgaaacaccg aacggcctct
    14101 tcgtggttga acgcgtacgc ccacacgagg ccccggtcga accagatttg cgcctgggga
    14161 gacggcgtct cgatcgggcg atggtaggag ccgaggtcga agtacggctc ggttccgccc
    14221 agcgaaacac tctgggaagc ggtcatattc ggacgatagc gtgaccgtta gccggacgaa
    14281 ccgagatcgg cgacacagcg aaaaccgatg tgcgtcgtgg cggtgtcgcg cgactgcggc
    14341 gagcgcgccg cgggccggta ccggtggcag tactcgggtg cacacagatg cgagccgccc
    14401 ttcaatgtct ggctgacggt cggatcggcc gacgacaccg acggtccgca acacggctgc
    14461 ccgacccggt ggtgcgtgga gtattcggtg gtcgtccact cccacacgtt gccgatcatg
    14521 tcgagcagcc cgaacccgtt ggcggggaac gtgcccaccg gcgaggtgcc gacccagccg
    14581 tcggcgccgt cattgcggta cgggaaccgg ccctgccacg tgttggccat caagtgcccg
    14641 cccggcgtcg gctcgtcgcc ccatgcgtac accgacgagc agccggcccg cgcgacgtac
    14701 tcccactcgg cttccgtcgg caggcgccga cccgcccact tcgcgtacgc ccacgcgtcc
    14761 gggtaggcga cctgcacgac gggatggtca ggcttgtcct cgactgagct gcccggcccg
    14821 aacgggtgcc gccagctcac gcccggcgcc cagtcccacc actgtcgcca gtcacgcaga
    14881 tcgaccgggc cgctgctggg ccggaacacc atcgcgccgg gaaccaggtc ggcggtggcc
    14941 gccccggggt acagggcagg gtccagcggc tgctccgcga ccgtcacata gcctgtggcg
    15001 gacatgaatt cggcgaactg ggcgttggtc accgggtggc gctcgatcgc gaacccggcc
    15061 accgtggccg tatggaccgg cgcctcttcc gggtagaagc tcgtcgaacc catgcggaaa
    15121 gacccgccag gcagttcgac aagctcggtc agcatgtgcc cagcgtatta gtccttcgcg
    15181 aacgccagcg ccagttcctt ttccacgtgc tggtaaggct cgccggagac gtcgacgacg
    15241 acctgctgga tcgtgccgcc ggtgaacgca tacggcgcct tataggagct ggacaccgcc
    15301 gaccccgcgt tgcgaccgac cgagaggctg gcaccggcga gaccgaatgt tccgggatgg
    15361 gctttcacgt tcggccgcgt gccgacagct tgctcgtcga tgtagaccgt gacctcgcca
    15421 agcggggtgt ggctgccctc gacggtccct tgcagggtgt agcggacccc gaagatgtgc
    15481 ttacccaacg gaacagcgcc ggacgacgac accttctgct cgtcttctcc catgaagttg
    15541 tagatgtaat gcagtcggcc gtcctgaacg aacagcacat gtccgccgtg cgcgccgccc
    15601 tgcttgaaga tcacgccttc ggcgccagtg gtgtcgaccg tgacctcggc cagcacggag
    15661 aacgactggc cgcggatctc aacggccgcg ccgatcccga cctcggcggt gttcgggtag
    15721 tagacgaacg actttcgatc gccggcgaga tacggccgcc atcgggtcaa cgtctcgaga
    15781 aggttcaagt cggccagcgg caagccgttg tacttggccg cctcgtcgaa ccagagcttc
    15841 gtcaactcca cgagtttcga agactctttc tcggcgaggt cgtggcattg gctgcggtcg
    15901 gcctcgatgt ggaacaactc ccagcggtcc ttgtcgaagt gcgaccagcc cgacggcgtc
    15961 gccgcgtgca cggtgttggc gaaccagccc ttgtgccaga tgccgcgggt gccgagcatt
    16021 tcgtagaact gcgtgtcctt tccggtgtcg gccttcggat cgtcaagtgc cgctttgaaa
    16081 ctcatgccgt cgagcgcctt ctgcgcgatg cccttgacgg tgtctggcgg tgtgatgccc
    16141 agcaggtcgt agaccgtggg tgtgatgtcg caaacgttga cgtagttgtc ccgaacctcg
    16201 ccgtgggcgg cgattccgtt gggccagctg atgatcgcgg tgtcggcgat gccgccctcg
    16261 tgggacgcgt agcgcttgaa cagcttgtag ggcgtgttga acgccatcgc ccagccgatc
    16321 gggtaatggt tgtaggtgtc ggtgccgccc agattgtcgt agaacctcat gctctcttcg
    16381 gcggtgtcga tgtagccgtt gaagaacttc acctcgttca ccgagccgtt cgggccgcct
    16441 tccccgctgg cgccgttgtc cgagatcact acgatgaccg tgttgtcgag ctgaccggac
    16501 tcgtcgaggt agtcgaggat gcggccgagt tgcgcgtcgg tgtagctgag gaagcccgcg
    16561 aacacctcgg ccatccggca gaacagcttc ttctcctcgt cgcccagcgt gtcccacggg
    16621 cgcacggtgt cctgcagcgg ccacggttcg ccgttgggcc ccttgacatc caggtagggg
    16681 ttgactggcg acaactcggt gtcgggcggc acgatgccca acttcttctg gttttccagc
    16741 gcgatctcgc ggtaccgctc gtagccaatg tcgaacttgc ccgcgtactt gtccgcccat
    16801 tccttgaaga cgtggtgcgg cgcgtgtccg gcgccggggc agacgtacga gaaccacggc
    16861 ttgtccggcg cgatcatctt cgcatcgcgg atgaactcaa tggtcttgtc cgcgatgtct
    16921 tttgacaggt gatagccatc ttccggcgtc gcgggcgggg tgacgggatg gttgtcgtaa
    16981 accaagtcgg gataccactg gtcggtctcg ccgcccatga agccgtagaa ccgctcgaat
    17041 ccgcgggcca gcggccaatg ccgcttggtg gcggccaggt tcgactcctc gagcggggtg
    17101 agatgccact tgccgacgca gtaggtgttc cagccctgtt cggcgagcac ctccgaaatc
    17161 agcgcggtgt cgaacgggat acgcccgttg cagttcggga agccgtcggt gaactcttcg
    17221 atggtcgcca tcccgaccgt ggtggcgttg cgtccggtca gaagcgacgc ccgcgtcggc
    17281 gagcacagcg cggtggtgtg gaactgcgag agccgcaccc cgcgctcggc gatgcggctc
    17341 atcgtgggca tctcgaccag cccgccgaag cagtcccaag tggcgatgcc ggtgtcgtcc
    17401 cagacgacgt agagcacatt gggcgcgccg tcgggtgcgg tcggcgcggc gtacggcccc
    17461 cagtccggtt cggagtttct gatgtccagc tcgatcttgc ccgcaaactc cgacgccatg
    17521 tccggctccg ttctgctcgc actcgtcccg acgtggcccg gacactacac cgggaacgta
    17581 gatgtgagct taagaaattg ccgagtgcat ctgttacgaa agccgatgcg cccgacgcgt
    17641 atcgatgctc tcggctcgcg ccgaacgtga gcccactgcg aagattcgaa atcttcgcag
    17701 tggccgcacg ctcgcggtga acatggcgcc gcccggcttc gcgccgagac gtcagctcgc
    17761 cggcaccggt ttgttcaccc ggtacaaccg ccaggtcggt tggctgtcac cgttgttcgg
    17821 gacctccagt tccagcgttg caatgtccgc gccggtgtca tacagcgtcg ggtagcgcag
    17881 gttgagcgca tcggacgcgc cgcgaccggt cgggggtacg gcgagcaggt actcgatccc
    17941 gccggttacc ggatcgttga gcagtgcggt gaagtcgggg tcggacggaa cgatgaaggt
    18001 cctcggccgt tcggatgcgg cgacgatggc gaacccgtac acggtgtcgg tcagcaccga
    18061 actctccggc aggttcagcc ggccgaggta cccggcgatg tcccgctcgg tggaaaacga
    18121 tgccgcgatg cggtgctcga tggccttgcg ctcggtgacg ttgtcgggct gaggcgccag
    18181 cacggcgccg agcgcatact cctgcggcgc gtacttcggt tggcccatcc cccacgcggt
    18241 gaccggtacg cacaccgcaa acgcaagcag cacaacggca tacgcgaacc gccaccgacc
    18301 gctgaccggc actggttccg gtgacgccag cgcgtacttg ccgcgccgcg tggccggcga
    18361 caccacaccg tcgggaacag ccagcagtgc caaggtcgca gcgaacggta ctgcgacgat
    18421 gtagaaacgc aggaacccga atgtcgaacc gctggcatag ctcaacgcct gaaagctcag
    18481 cgccgcaccg tagagcgccg ccggcacgat cacggtctgc caggtgtgcc agcgccaccg
    18541 cgcgacagcg gtccaagccg cgatgggcgc cagtgtcggc gcgagcagtc caatgcaaat
    18601 cagcgcgaac atgagcccgc ccgtcaaacc ggcggaggtg ctgcccgact gctgcagaat
    18661 cgcggcattg ccgtactgcg agctgaattg ggcga
//
LOCUS       DASWBF010000534         8645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1041207,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000534 DASWBF010000000
VERSION     DASWBF010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041207"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VF874_20000"
     CDS             <1..1416
                     /locus_tag="VF874_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX9178258.1"
                     /translation="LWMRGASLTMLHQPTPRTDLQVWAQDTITVIDMIKAKVVIVSDP
                     FMAAAPVLADRGIEVVTVEKLLGHAPIAPVDTGEEDLALLQLTSGSTGSPKAVSITHR
                     NVVCNAEAMSIGAHYNIDTDVIVSWLPLFHDMGMTGFLSVPMYFGAELVKITPMDFLS
                     DILLWAKLIDKYRGTMTAAPNFAYSLLAKRLRKHAKPGQFDLSTLRWALSGGEQVEPA
                     DVEDLCEAGAPFGLRPEAILPAYGMAETTVAVSFSECGAGLMVDEVDADILAMLRRAL
                     PATMGNTRRLAALGPLLGGLEARIIDDDGSLLPARGVGVIELRGEPLTRGYITTGGFV
                     AAQDERGWYNTGDLGYLTEHGHVVVCGRVKDVIIMAGRSIYPADVERAAGRVEGVRPG
                     CAVAVRLDAEHSRESLAVAVESSMFEDPAEVRRIKHQVAHEVVTEVDVRPRNVVVLGP
                     GVIPKTPSGKLRRAEALAFLS"
     gene            1814..2317
                     /locus_tag="VF874_20005"
     CDS             1814..2317
                     /locus_tag="VF874_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX9178259.1"
                     /translation="MPRSFDFSIESSASVEQIHSAFSEEDYWLARLAAFGGIGRLDSL
                     IIGTDGSVTVVIIQDLRHEGLPGLVAKFYPRDWQVVHNETWSPIGGGLVRGEVSIAAR
                     GAPGSGFGTALLAPAQNGARLKCTATVEFKVPLVGGKIESLIGRQLTEGYSVIQRFTT
                     KWIKEHA"
     gene            complement(2548..3864)
                     /locus_tag="VF874_20010"
     CDS             complement(2548..3864)
                     /locus_tag="VF874_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltokinase"
                     /protein_id="HEX9178260.1"
                     /translation="MSLPFAEWLPRQRWYAGRSRELSAAEPARVVPLRDDIELMLVDA
                     SYIDGSSERYQVIVRWDVAPPDEYNTVATIGSAWDRTGYDALYDPQAAQFLLSLIDSS
                     AVRDDVTFAKEPGATLPIDAAPRVFDSEQSNTSVIFEQEAILKVFRRVAPGVNPDIEL
                     NRVLGRAGNPHVAPLLGSIEMSLAGQDWPLGMVTKFAANSAEGWDMATASTRDLFAEG
                     DLYAYEVGGDFAGESHRLGEAVASVHGTLADTLGTSTVEFSVDAMLARLSSAAAAVPQ
                     MRQYVPAVEERFHKLVGETITVQRVHGDLHLGQVLRTPETWLLIDFEGEPGQPLEERR
                     KPDSPLRDVAGMLRSYEYAAYQRLVGHDGDQAKQLAARAREWVERNQSAFCDGYAAVA
                     EVDPRDAATVMLAYELDKAVYEAAYEARHRPTWLSIPLRSIARLVS"
     gene            complement(3861..5627)
                     /gene="treS"
                     /locus_tag="VF874_20015"
     CDS             complement(3861..5627)
                     /gene="treS"
                     /locus_tag="VF874_20015"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX9178261.1"
                     /translation="MTHDADDPDPDPVEGSHVEDGVVEHPTAEDFGHARVLPADPMWF
                     KRAVFYEVLVRAFYDASADGSGDLRGLTEKLDYLQWLGVDCLWLPPFYDSPLRDGGYD
                     IRDFYKVLPEFGDVDDFVALLDAAHRRGIRVITDLVMNHTSESHPWFQESRRDPDGPY
                     GDFYVWSDTSDRYADARIIFVDTEESNWTFDPVRRQFYWHRFFSHQPDLNYDNPAVQE
                     AMIDVLRFWLDLGIDGFRLDAVPYLFEREGTNCENLPETHAFLKRCRQVVDDEFPGRV
                     LLAEANQWPADVVEYFGDPDTGGDECHMAFHFPLMPRIFMAVRRESRIPISEILAQTP
                     PIPKMAQWGIFLRNHDELTLEMVTDEERDYMYSEYAKDPRMKANVGIRRRLAPLLDND
                     RNQIELFTALLLSLPGSPVIYYGDEIGMGDIIWLGDRDGVRTPMQWTPDRNAGFSKAN
                     PGRLYLPPNLDPVYGYPSVNVEAQIDSSTSLLNWTRTMLGVRRRHPAFATGTFKELGG
                     SNPSVLAYLREEDGDLVFCVNNLSRFPQPIELYLQHWNGYVPVELTGNVEFPRIGHLP
                     YLLTLPGHGFYWFQLRVSEEKQ"
     gene            5764..6651
                     /locus_tag="VF874_20020"
     CDS             5764..6651
                     /locus_tag="VF874_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HEX9178262.1"
                     /translation="MADRPTDNGALTPHFDDVQAHYDLSDDFFRLFLDPTQTYSCAYF
                     ECDDMTLEEAQLAKIDLALGKLGLEPGMTLLDVGCGWGSTMKRAVQRYDVDVVGLTLS
                     RNQAVHVERLLADLPGRRTKRVLLHGWEEFDEPVDRIVSIGAFEHFGHERYPAFFDFA
                     FSALPSDGIMLLHNITGLHPTQMAERGMPLSFQFARFLKFMLTEIFPGGRLPSIEMVQ
                     ERAGEGGFTVARIQSLQPHYAKTLDRWAEALAAHKHEAIAIQSDEVYERYMRYLTGCA
                     DMFRVGYIDVNQFTLAKTP"
     gene            complement(6656..6844)
                     /locus_tag="VF874_20025"
     CDS             complement(6656..6844)
                     /locus_tag="VF874_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178263.1"
                     /translation="MAKVDVSVPSDMSPGSQFHLCADLSGGVLSGPVGKLVARVLKGD
                     VRKSVENLSHLQSTASSR"
     gene            6933..7730
                     /locus_tag="VF874_20030"
     CDS             6933..7730
                     /locus_tag="VF874_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEX9178264.1"
                     /translation="MQRDHGDPGDAPSAPPPLTPVVNPRRPSAAEEARTITASTNMGT
                     LATLTSTGDPWASFVTYGLLDGAPVLCVSRMAEHGRNLDHDHRASLSIVAPSTDRDPL
                     ASGRITLAGKAERPGGDELAAARDAHLAAVAAAKYYIDYSDFATWVLRVHRVRWVGGY
                     GRMDSATGEDYMAATPDPVQPLADAAIEHLNADPADSLAEMAKAFGGFPDTDAATCTG
                     ADRYGLDLRVSTKRGIAYTRVGFVDTIDSVDELRAATVELVRRARAS"
     gene            7727..8518
                     /locus_tag="VF874_20035"
     CDS             7727..8518
                     /locus_tag="VF874_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9178265.1"
                     /translation="MKVHHLNCGTMRPPGTPALVCHVLLVETDNGLVLVDTGYGLADI
                     ADPRRRIGPMRHLLKPAFDPDETAARQVQRLGFSRDDVRHIVITHFDLDHIGGLADFP
                     AAQVHVTTAEIDGSIRSPSWRERVRYKAVQWEHHPRVVEYSSDGKSWRGVSAANQLSA
                     VSAGIVLVPLSGHTRGHACVAVDAGDHWILHCGDAFYHRGEIDGRTSVPRSLRLQERI
                     TAFDFKRLRDNQARLAELFARGEPDLLMICAHDSNQYERAQNRQA"
BASE COUNT         1485 a         2805 c         2875 g         1480 t
ORIGIN      
        1 ctgtggatgc gcggcgccag cctgacgatg ctgcatcagc cgacgccgcg caccgatctg
       61 caggtgtggg cgcaggacac catcacagtg atcgacatga tcaaggcgaa ggttgtcatc
      121 gtgtccgatc ccttcatggc ggccgcgccg gtgctggcgg accgcggcat cgaggtggtc
      181 accgtcgaga aactgctcgg tcatgccccc atcgccccgg tcgacaccgg cgaggaagat
      241 ctggcgctgc tgcaattgac atccgggtcg acgggatccc cgaaggcggt gtcgattacc
      301 caccgcaacg tggtctgcaa tgccgaggcg atgtccatcg gcgcccacta caacatcgac
      361 accgacgtga tcgtgagctg gctgccgctc ttccacgaca tgggcatgac cggttttttg
      421 agtgtgccga tgtactttgg cgccgagctg gtcaagatca cgccaatgga tttcctgagc
      481 gatatcttgt tgtgggccaa gctgatcgac aaataccggg gcaccatgac cgctgcgccg
      541 aacttcgcct attcgctgtt ggcgaagcga ctgcgcaagc acgccaagcc cggtcaattc
      601 gatctgtcca cgctgcgatg ggcactgtcg ggtggcgagc aggtggagcc cgccgacgtc
      661 gaggatctct gtgaggccgg cgcgccgttc gggcttcggc cggaggcaat cctgcccgcc
      721 tatggcatgg ccgagacgac tgtggcggtg tcgttttccg agtgcggcgc ggggctgatg
      781 gtcgacgagg tcgacgccga catcctggcc atgctgcgac gggcgttgcc ggccactatg
      841 ggtaacaccc gccggctggc ggcattgggc ccgctcctgg gcggactgga agccaggatc
      901 attgatgacg acggcagcct gctgccggcg cgcggcgtcg gggtgattga gctgcgcggt
      961 gagccgttga cacgcggcta catcacgacg ggtggattcg tcgctgcgca ggacgagcgg
     1021 ggctggtata acaccggtga tctgggttat ctgaccgagc acggacacgt cgtggtctgc
     1081 ggccgggtga aggatgtgat catcatggcc gggcgcagca tctacccggc cgacgtcgag
     1141 cgggctgccg ggcgggtcga gggggttcgg cccgggtgtg cggttgcggt gcggttggat
     1201 gccgaacatt cgcgggaatc tttagcggtc gcggtggagt cgagcatgtt cgaggatccg
     1261 gcagaggtgc gccgcatcaa acaccaggtc gcccacgagg tcgttacgga ggtcgatgtg
     1321 cggccgcgta acgttgtcgt gctgggtccg ggcgtcattc cgaagacacc gtcaggaaaa
     1381 ctacgccgcg cggaagcgct ggcgtttctc agctaagcgg catggccgag atgacgccaa
     1441 ctgaacagac gctggcgccg ataacgagcg tttcgtggtc gcgggtggtc ctcaagcgtt
     1501 accaggcacg cacaagatca acgggcaatg cgattcctct gtcgtcccgc gcccgtgggc
     1561 ggtcgatctc agcagtgaag ggttgatctt tcgacccggg ctggttccag cgcccggtgg
     1621 ggttgttggc cgggatcggc gcccgcggaa gtgggttccg tggagcgttg gcgccgcgcg
     1681 gccgcccctg tggatttcag attcgggtag ggtctggcaa tgcctgagga ttgccaggcc
     1741 tcaaggcacg tgaactatcc cctgccaaag cgacacctgg ccggggccga ccgacagcga
     1801 tagcgtggct gctatgccac gatcattcga cttctcgatc gaatcctcgg ccagcgtcga
     1861 acaaatccat tcggcgttct ccgaggagga ttactggctg gcgcggctgg cggccttcgg
     1921 cggcatcggc aggctggact cgttgatcat tggcaccgat ggctctgtga ccgtggtcat
     1981 tatccaggac ctgcgccacg aaggactgcc cgggctggtc gccaagttct atccccgtga
     2041 ttggcaagtc gtgcacaatg agacgtggag cccgatcggc ggcggcctgg tgcgcgggga
     2101 ggtcagcatc gcggcgcggg gagcgccagg atccgggttc ggcacggctt tgctggcacc
     2161 ggcgcagaac ggcgcgcgcc tgaagtgcac ggcgaccgtg gagttcaaag tcccattggt
     2221 cggcggcaag atcgagagcc tcatcggccg ccagttgacc gaagggtatt cggttataca
     2281 gcgcttcacc acgaagtgga ttaaggagca cgcctgacgc cggttgtgtg gcggtgtaac
     2341 cggccatccg gcctcctgcg acagttctgt tggatcacct taatgtcgtc gcctacccat
     2401 gattaaagcg gcccgacggg ttcgtgcacg tcggtggcgg tgtttgctgg tcccgcaact
     2461 gaaccgacga ttgtctcgat atcggccgcc cgtactgccg cagtagctta agtttcggcg
     2521 gccgccggca gtaggcaggc ggcacattca gctgaccagc cgcgcgatcg accgcagcgg
     2581 gatcgacaac cacgtcggcc ggtgccgcgc ttcgtacgcg gcctcgtaga cggccttgtc
     2641 caactcgtag gccaacatca ccgtcgccgc gtcacgcgga tccacttccg ccaccgccgc
     2701 atacccgtcg cagaacgcgg actggttgcg ctccacccat tcccgcgccc gggcggccaa
     2761 ctgcttggcc tgatctccgt cgtggcccac cagccgttga tatgccgcgt actcatacga
     2821 ccgcagcatc ccggcgacgt cccggagcgg cgagtcgggc ttgcgcctct cctccagcgg
     2881 ctgccccggt tcgccctcga agtcgatcag cagccaggtt tcgggggtgc gtagcacctg
     2941 gcccaggtgc aggtcgccgt gcacgcgctg cacggtgatc gtctcgccga cgagcttgtg
     3001 gaacctctct tcgaccgcgg ggacgtactg ccgcatctgc ggtaccgccg ctgcggcgga
     3061 tgagagccgc gccagcatcg cgtcgacgga gaactcgacc gtcgaggtcc ccaatgtgtc
     3121 ggccagcgtg ccgtgcacgg aagcgaccgc ctcgccgagt cgatgcgact cgccggcgaa
     3181 gtcaccgccg acctcgtagg cgtatagatc gccctccgca aacagatcgc gggtgcttgc
     3241 cgtcgccatg tcccagcctt cggccgagtt cgcggcgaac ttcgtcacca tccccagcgg
     3301 ccagtcttgg ccggccaggc tcatctcgat cgacccgagc agtggagcga cgtgcggatt
     3361 gcccgctcgg cccagcaccc ggttcaactc gatgtcgggg ttgacgccgg gcgcgacgcg
     3421 gcgaaacacc ttgaggatgg cctcttgctc gaagatcacg ctggtgttgc tctgttcgga
     3481 gtcgaacacc cgcggcgcgg cgtcaatcgg cagcgtggca cccggttcct tcgcgaacgt
     3541 cacgtcgtca cgtacggccg acgagtcgat cagcgacagc aggaactgtg ccgcctgcgg
     3601 gtcgtagagc gcgtcgtacc cggtgcgatc ccaagcgctt ccgatcgtcg cgacggtgtt
     3661 gtactcgtcg gggggcgcga cgtcccagcg cacgatcacc tgatagcgct ccgacgaccc
     3721 gtcgatatag gacgcgtcga ccagcatcag ctcgatgtcg tcgcgaagcg gcacgacccg
     3781 ggccggttcg gcggcggaca gctctcggct gcggccggcg taccagcgct gacgcggaag
     3841 ccattccgcg aagggcaggc tcattgcttc tcctcggaca cgcgcaattg aaaccagtag
     3901 aacccgtggc ccggcagcgt caacaagtag ggcaggtggc cgatgcgcgg gaactctacg
     3961 ttgcccgtca gctcgaccgg tacgtagcca ttccagtgct gcaggtacag ctcgatgggc
     4021 tgggggaagc gggacaggtt gttgacgcag aacacgaggt cgccgtcctc ttcacgcaga
     4081 tacgccaaca ccgacgggtt cgagccgccg agctccttga acgtgcccgt ggcgaacgca
     4141 gggtgccggc ggcgcacgcc cagcatcgtg cgggtccagt tcaaaagcga tgtagagctg
     4201 tcgatctgcg cctcgacgtt gaccgacggg tatccgtaga ccggatccag gttcggcggt
     4261 aggtacagcc gccccgggtt ggccttggag aagccggcgt tgcggtcggg ggtccactgc
     4321 atcggcgtgc gcacgccgtc gcggtcgccg agccagatga tgtcacccat cccgatctcg
     4381 tcgccgtagt agatgaccgg ggagcccggc agcgacagca gcagcgcggt gaacagctcg
     4441 atctggttgc ggtcgttgtc cagcagcggg gccagccgcc tgcggatccc gacgttggcc
     4501 ttcatccgcg ggtctttggc gtactcggag tacatgtagt cgcgctcttc gtcggtgacc
     4561 atctcgagcg tcagctcgtc gtggttgcgc aggaagatgc cccactgcgc catcttcggg
     4621 atcggcggcg tctgcgccag aatctcgctg atcgggatcc gcgactcgcg ccgcacggcc
     4681 atgaaaatgc gcggcatcag cgggaagtgg aacgccatgt ggcattcgtc gccgccggtg
     4741 tccgggtcgc cgaagtactc gaccacgtcg gccggccact ggttggcctc ggccagcagc
     4801 acccggccgg ggaactcgtc gtcgaccacc tggcggcagc gcttcaggaa cgcgtgcgtc
     4861 tcgggcaggt tctcgcagtt ggtgccttcc cgctcgaaca ggtaaggcac ggcgtccagc
     4921 cggaagccgt cgatgccgag gtcgagccag aaccgcagga cgtcgatcat cgcctcctgc
     4981 acggccgggt tgtcgtagtt gaggtccggc tggtggctga agaaccggtg ccagtagaac
     5041 tgccggcgga ccgggtcgaa ggtccagttc gactcctcgg tgtcgacgaa gatgatccgc
     5101 gcgtcggcgt agcggtcgct ggtgtcgctc cagacgtaga agtcgccgta cgggccgtcg
     5161 gggtcgcgcc gggattcctg gaaccacggg tgcgattcgg aggtgtggtt catcacaagg
     5221 tcggtgatca cgcgaattcc ccggcggtgt gccgcgtcca gcaacgcgac gaagtcgtcg
     5281 acgtcgccga attcgggcaa caccttgtag aagtcgcgga tgtcgtagcc gccgtcgcgc
     5341 agcggcgagt cgtagaacgg cggcagccac agacagtcga cgcccagcca ctgcaggtag
     5401 tccagcttct ccgtcagccc ccgcaggtcg ccggaaccgt cggcgctcgc gtcgtagaag
     5461 gcccgcacca gcacctcgta gaacacggcc cgcttgaacc acatcggatc cgccggaaga
     5521 acccgcgcgt gcccgaagtc ctccgcggtg ggatgctcca ctactccgtc ttcgacgtgg
     5581 ctgccttcga cagggtcagg atcagggtcg tcggcgtcgt gtgtcatctg catcagagtg
     5641 tcacctaccc ggcagcgtgc ttgctgaacc cacggggcga gattcacgct agcgcgcaga
     5701 ggtgcgagta gctaacgcgc tagcgtcagt ttcggcgagc atgcccaatg acgaggagcc
     5761 aacgtggccg accgaccaac cgacaacggc gcactcacgc cgcacttcga cgacgtccag
     5821 gcccattacg acctgtctga cgactttttt cggctcttcc tcgatccgac ccagacctac
     5881 agttgcgcgt acttcgagtg cgacgacatg acgttggaag aggcgcagct cgccaagatc
     5941 gacctcgcgt tgggcaagct gggcctcgag ccggggatga cgttgctcga cgtcggttgc
     6001 ggatggggct ccacgatgaa gcgcgcggtt cagcgctacg acgtcgacgt cgtcggcctg
     6061 acgttgagca ggaaccaggc cgttcacgtc gagaggctgt tggctgacct gcccggtcgg
     6121 cgcaccaagc gggtcctgct gcacggctgg gaagagttcg atgagccggt ggaccgcatc
     6181 gtgtcgatcg gcgcgttcga gcatttcggc cacgagcgct acccggcgtt cttcgacttc
     6241 gctttcagcg cactgccgtc agacggaatc atgctgctgc acaacatcac tggcctgcac
     6301 cccacccaga tggccgagcg gggcatgccg ctgtcgttcc agttcgcccg gttcctcaag
     6361 ttcatgctga ccgagatctt ccccggcggc cggctgccgt cgatcgagat ggtgcaggaa
     6421 cgtgccggcg aaggcggatt cacggtcgcc cggatccaat cgctgcagcc gcattacgcc
     6481 aagacactcg accggtgggc cgaggcgctg gccgcgcaca agcacgaggc catcgcgatc
     6541 cagtccgacg aggtgtacga gcggtacatg aggtacctga ccggctgtgc cgacatgttc
     6601 cgcgtcggct acatcgacgt caaccagttc acgctggcga agacaccatg aagttctagc
     6661 ggctgctcgc agtcgactgc aagtgcgaca ggttctccac cgacttgcgc acgtctccct
     6721 tgagcacccg cgccaccagc ttgccgaccg gccccgacag cacgccgccg ctcaggtccg
     6781 cgcagaggtg gaattgcgag ccggggctca tgtcggaggg caccgacacg tcaactttgg
     6841 ccatgttctt tccctacccg gctgaccgca tgtgtaaccg gcgcccggcc cgcgaatcgc
     6901 gcgccgcggg ccggtatcgt accgacgtga cgttgcaacg cgatcacggc gacccgggcg
     6961 acgctccgtc ggcgccgccg ccgctgacgc cggtcgtcaa tccccgccgg ccgtcggcgg
     7021 ccgaggaggc gcgcacgatc acggcgtcaa ccaacatggg aacgctcgca acgctcacgt
     7081 ccaccgggga cccgtgggcg tcgttcgtca cctacggcct gctggacggc gcgcctgtgc
     7141 tgtgcgtatc ccggatggcc gagcacggcc gcaacctcga ccacgaccat cgggcgagct
     7201 tgtcgatcgt cgcgcccagc accgatcgag atccgctggc cagcggccga atcacgctgg
     7261 cgggcaaggc cgaaagacca ggaggcgacg agcttgccgc agcccgcgac gcgcatctcg
     7321 ctgcggtcgc cgcggcgaag tactacatcg actacagcga cttcgcgacg tgggtgctgc
     7381 gagtgcatcg ggtgcgatgg gtgggcgggt acggccggat ggattcggcg acgggtgagg
     7441 actacatggc ggcaacaccg gacccggtac agccactcgc cgacgcggcg atcgagcacc
     7501 tgaacgccga ccccgccgac tcgctcgctg agatggccaa ggcgttcggc ggattccccg
     7561 acacggacgc cgcgacctgc accggcgccg accgctacgg actggacctg cgagtcagca
     7621 ccaaacgcgg aatcgcctac acccgtgtcg gattcgttga tacgatcgac tcggtcgacg
     7681 agttgcgcgc cgccacagtg gaattagtgc gacgggcacg cgcgtcatga aagttcacca
     7741 cctcaattgc gggaccatgc ggccacccgg cacgccagcg ctggtctgcc atgtgctgct
     7801 ggtggagacc gacaacggcc tcgtgctggt cgacaccggc tacgggttgg ccgacatcgc
     7861 cgacccgcga cggcggatcg ggccgatgcg ccacctgctc aagcctgcgt tcgaccccga
     7921 cgagaccgcc gcacgacagg tgcagcgcct cggattcagc cgagacgacg tgcggcacat
     7981 cgtgatcacg catttcgact tggaccacat cggtggcctg gcggatttcc cggccgcgca
     8041 agtacacgtg acaaccgctg agatagacgg gtcgatcaga tcgccgtcgt ggcgagaacg
     8101 ggtgcggtac aaggctgttc aatgggagca ccacccgagg gtcgtcgaat acagctcgga
     8161 cggcaaatcc tggcgcggag tctctgcggc gaaccaactc agtgcggtgt cggccggaat
     8221 cgtgttggtt ccgctgtccg gccatacccg cggtcacgcg tgcgtggcgg tggacgccgg
     8281 tgaccactgg atcctgcact gcggcgatgc gttctaccac cgcggcgaga tcgacggccg
     8341 tacgtcggtg cctcggtcgc tcaggctgca agagagaatc accgcgttcg acttcaaacg
     8401 actacgcgac aaccaggccc ggctcgccga gttgttcgct cgcggcgaac ccgacctgct
     8461 gatgatctgc gcgcacgact cgaaccaata cgagcgcgca caaaaccgcc aagcctgagc
     8521 aacatcctgc cagcccggaa ctaaagggtc cggcctcccg tgttctgaaa gccagacacc
     8581 gaggaggcag aaatgaagag gtccacattc gccacgatcg cagcggccgg cttggcggcc
     8641 atcac
//
LOCUS       DASWBF010000535        12555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1041575,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000535 DASWBF010000000
VERSION     DASWBF010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12555
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041575"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /gene="dnaB"
                     /locus_tag="VF874_20040"
     CDS             complement(<1..1592)
                     /gene="dnaB"
                     /locus_tag="VF874_20040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9178266.1"
                     /translation="MAVVDDLGRSGMGTPPSNEEFGRQPPQDIVAEQSVLGGMLLSKD
                     AVADVVEKLRPGDFYRPAHQNIYDVIVDLSTRGEPADPVTVAAELDRRGLLRRIGGAP
                     YLHTLISTVPTAANAGYYADIVAEKALLRRLVEAGTRVVQYGYAGAEGSDVAEVVDRA
                     QAEMYDVTDRRLSEDFVPLEQLLQPTMDEIDAIASSGGRASGVPTGFVELDDVTNGLH
                     PGQMIIIAARPGVGKALALDTPLPTPTGWTTMGDVAVGDELLDADGQPTRVVAATEAM
                     LGRPCYEIEFSDGTTIVADAAHQWPTGRGISVTAALRPGVDLISAARAPVLSVARGRQ
                     QSAWGIDSLRQVGSVPVRCVQVDNYAHLYLAGRAMMPTHNSTLALDFLRSCSIKQRMA
                     SVIFSLEMSKSEIVMRLLSAEAKIKLSDMRSGRMGDDDWARLARRMSQISEAPLYIDD
                     SPNLTMMEIRAKSRRLRQKADLKLIVVDYMQLMTSGKKFESRQQEVSEFSRSLKLLAK
                     ELEVPVVALSQLNRGPEQRTDK"
     gene            complement(2297..2752)
                     /gene="rplI"
                     /locus_tag="VF874_20045"
     CDS             complement(2297..2752)
                     /gene="rplI"
                     /locus_tag="VF874_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9178267.1"
                     /translation="MKLILTAEVDHLGSAGDLVEVKDGYGRNYLLPRGLAIVATRGAE
                     RQASEIRRARDAKTVRDREHAAELKTAIEKLGTVSLLARTAGDSGRLFGSVTAADVVS
                     AIKKAGGPNVDKRSVQMPQTHIKSLGSHPIVVRLHSDVQATVSLEVVAG"
     gene            complement(2776..3036)
                     /gene="rpsR"
                     /locus_tag="VF874_20050"
     CDS             complement(2776..3036)
                     /gene="rpsR"
                     /locus_tag="VF874_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX9178268.1"
                     /translation="MAKSNKRRPTRAPEKPIKTRKCVFCSKKGQAIDYKDTALLRTYI
                     SERGKIRARRVTGNCVQHQRDIAIAVKNAREVALLPFTSSTR"
     gene            complement(3068..3565)
                     /locus_tag="VF874_20055"
     CDS             complement(3068..3565)
                     /locus_tag="VF874_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9178269.1"
                     /translation="MAGDTTITVVGNLTADPELRFTPSGAAVANFTVASTPRIYDRQS
                     GEWKDGDALFLRCNIWREAAENVAESLTRGSRVIVTGRLKQRSFETREGEKRTVFEVE
                     VDEIGPSLRYATAKVNKASRGSSSGGFGSGSKQSGGQQSTPDDPWGSAPASGSFGGSD
                     DEPPF"
     gene            complement(3676..3966)
                     /gene="rpsF"
                     /locus_tag="VF874_20060"
     CDS             complement(3676..3966)
                     /gene="rpsF"
                     /locus_tag="VF874_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX9178270.1"
                     /translation="MRPYEIMVILDPTLDERTVAPSLEAFLNVIRNDGGKVDKVDIWG
                     RRRLAYEIAKHSEGIYAVVDVTAKPETVSELDRQLSLNESVLRTKVLRTDKH"
     gene            complement(4082..4408)
                     /locus_tag="VF874_20065"
     CDS             complement(4082..4408)
                     /locus_tag="VF874_20065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX9178271.1"
                     /translation="MSTRQNVRAVLDSAKASGARVIVLAQNEWFRSTEGQNYGTDLSA
                     QRDTHDTVLIEEASAHGTEFVDLRSLYRQQADHGQWVDDGIHPTPAAYEAWASELASA
                     LSAPCK"
     gene            complement(4476..4586)
                     /locus_tag="VF874_20070"
                     /pseudo
     CDS             complement(4476..4586)
                     /locus_tag="VF874_20070"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
     gene            complement(4618..5282)
                     /locus_tag="VF874_20075"
                     /pseudo
     CDS             complement(4618..5282)
                     /locus_tag="VF874_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912418.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
     gene            complement(5239..6867)
                     /locus_tag="VF874_20080"
     CDS             complement(5239..6867)
                     /locus_tag="VF874_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX9178272.1"
                     /translation="MSPQPLAADRRSLDDRDFPSRTDAMGAALSETIGGPVGRHALIG
                     RQGLFTPLRVMFLIGLVFLALGYSTKAACLQSTSTGPADERVANWDNNRAYYELCYSD
                     TVPLYTAELLNLGKFPYKSSWLESDSTGKPKTQYDGRPAVRYMEYPVLTGLYQYASMA
                     VAKTYTALTKILGIPVIAEVVMFFNISAFGLALAWLGVVWATASLAGRRIWDATLVAA
                     SPVLIFQIFTNFDALATAFAVGSMLAWARRKPVLAGVLLGLGIAAKLYPVLLLLPLLA
                     LGLRTGRLRSVGQTAGTAALTWFLVNLPVLVLFPRGWSEFFRLNARRGDDMDSLYNVV
                     KSFTGWSGFDGNLGFWEPPVVLNTVVAVLFLIACAAITYIALTAPRRPRVAQLVFLVV
                     AAFLLTNKVWSPQFSLWLVPLAVLALPHRRILLAWMTIDALVWIPRMFYLYGDQTRGL
                     PEQPFTITVLLRDLAVLALCALVVWQIYHPERDLVRFGGRVDDPSGGIYENTADAPPA
                     WLPSWLRPRIPAPREPEEVFDADRDRAVPTQHIP"
     gene            complement(6906..9188)
                     /locus_tag="VF874_20085"
     CDS             complement(6906..9188)
                     /locus_tag="VF874_20085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9178273.1"
                     /translation="MLPPVTANTEPLRDEVDVVKAALDRTPPPKPPPGGPPSGGPPPR
                     PTPGPMHPINWRRLRRGLIIAVTVLIVLPLVTFGMAYLIVDVPQPGDIRTNQVSTILA
                     SDGTTELAKIVPPEGNRVDVDINQVPQHVRDAVLAAEDRDFYSNPGFSFSGFARAVKN
                     NLFGGDTQGGSTITQQYVKNALVGSQRAGVGGVVRKAKELVIATKMSSEWSKDQVLQA
                     YLNIIYFGRGAYGIGAASKAYFNKPVEQLTVSEGALLAALIQRPSSLDPAVDPQGSEK
                     RWSWVLDGMVTIGALPASERAAQVFPPTVPSDQSRMESQTTGPDGLIEREVTRELLDL
                     FDIDEQTLNTQGLQITTTIDPQAQRAAEDAVNNYLDGQDPDMRAAVVSIDPKTGAVKA
                     YYGGPEANGFDFAQAGLQTGSSFKVFALIAALQQGIGLGTQIDSSPLNVNGVQITNVE
                     GEGCGTCNIAEALKRSLNTSYYRLMLKLKNGPSDVAAAAHDAGIAESFPGVEHTLSED
                     GKGGPPSNGIVLGQYQTRPIDMASAYATIAASGMFRTPHFVQKVVNAEGQVLFDAATS
                     ESGGEQRIDEAVANNVISAMQPIAGYSNGHNLAGGRPSGAKTGTTQLGDTGANKDAWM
                     VGFTPSLATAVWVGTVRGDKPLENKWGSPVYGSGLPSDIWKATMDGALEGTDNESFPK
                     PTSIGGYAGVPAAPPPPPPSSPSPTPPTPTPGEVIQPSIEVAPGITIPIGPPTTVPEA
                     PPPPGDAGAQGPLPGPPPPP"
     gene            complement(9295..9720)
                     /locus_tag="VF874_20090"
     CDS             complement(9295..9720)
                     /locus_tag="VF874_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 domain-containing protein"
                     /protein_id="HEX9178274.1"
                     /translation="MRLQRQVVDYALRRRSLLAEVYSGRTGVSEVCDANPYLLRAAKY
                     HGKPSSVMCPICRKEQLTLVSWVFGDHLGPVSGSARTAEELVMLAARFDEFSVHVVEV
                     CRTCSWNHLVKSYMLGAVRPPTRSRGTRTARTGARTASE"
     gene            9844..10695
                     /locus_tag="VF874_20095"
     CDS             9844..10695
                     /locus_tag="VF874_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX9178275.1"
                     /translation="MATRQSVGTRAKDSDRNDTCQVLDTALSEGQLSMEEHRQRVSAA
                     TSAATLGELQALVADLQTSNAPVQLPKLKSRNQIQGGGGWGMRIAVAAVFVLLGVGLG
                     WGLFGSSNSPLAADPGAAPDGIAANTQAPPRLLHSANGLNGLFAQARKKWGDTMGYSL
                     TIYPDYASMERADPNDARRTLRYLYRGGWDDNPSVSAISDGEKLVDLAKFDVNAIIGR
                     LRGAPQILGIEQSEVKSIYLNIEPSKDPTTPDAVVISIYVSTTYEKSGYLTIDPQGEI
                     LSQNPPT"
     gene            10801..10953
                     /locus_tag="VF874_20100"
     CDS             10801..10953
                     /locus_tag="VF874_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178276.1"
                     /translation="MDIWLQQQAKAVLTALRRAEQLYGGPTPPQGSRPFAPRPDLEAD
                     LGGGLM"
     gene            10961..11746
                     /locus_tag="VF874_20105"
     CDS             10961..11746
                     /locus_tag="VF874_20105"
                     /inference="COORDINATES: protein motif:HMM:NF022231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4226 domain-containing protein"
                     /protein_id="HEX9178277.1"
                     /translation="MTQPAPSPSLGTDEQLSGRAADAVADMQSELQRRYSGLNGAEAQ
                     LAEALLTAQAASEAGRARLQYVQRQLIEAINNPVNTLDTPAGERQFLIFLRGKIAEIQ
                     RVVDDGALTAHDQAELARALGSDYLTGHLVDDPSSSPDPLPATNPARQPTPATPAGVG
                     SLPPVSGLPQVASPSASPPSGLGGSASPLAGLASLLAGQQQEEPMAAAPHTTNDERDE
                     GEGGEVTATEDDEREPDDDEAPVTPDDAPPGDTGPAETRPAAS"
     gene            11756..12058
                     /locus_tag="VF874_20110"
     CDS             11756..12058
                     /locus_tag="VF874_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HEX9178278.1"
                     /translation="MAERDIRVTPDVLRQIAARHDRIADEIASARSARQDILSAVATY
                     GPIMHTFKDAVADVVARRDAAFATHEADHRLVADKLRDAANRFGEQEDINASELQF"
     gene            12055..12330
                     /locus_tag="VF874_20115"
     CDS             12055..12330
                     /locus_tag="VF874_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2694 family protein"
                     /protein_id="HEX9178279.1"
                     /translation="MSDESFTASTPDNTVFVRVAVAGHTLGVQIEPGALRLSAHTLAE
                     RIMACNDVAYLKGQLAIRAELQQAAIPVDDMPTEDDLAAAERRLKEL"
     gene            12337..>12555
                     /locus_tag="VF874_20120"
     CDS             12337..>12555
                     /locus_tag="VF874_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178280.1"
                     /translation="MKVQVETSGLVAAADRLRAAATQLTGAGVPVHPPLARDQTSVAA
                     AARLSAAATALQSAAATHAAALIATAEHL"
BASE COUNT         2139 a         4157 c         4099 g         2160 t
ORIGIN      
        1 ttcttgtcgg tgcgctgctc tgggccacgg ttcagctggc tgagcgccac gaccggcacc
       61 tcgagttcct tcgccaacag cttgaggctg cgcgagaact ccgacacctc ctgttgccgc
      121 gactcgaact tcttacccga cgtcatcagc tgcatgtagt cgacgacgat cagcttcagg
      181 tcagccttct ggcgcagacg ccgcgacttc gctcggatct ccatcatcgt caggttcggc
      241 gaatcgtcga tatacagtgg cgcctcgctg atttggctca tccgtcgcgc gagccgtgcc
      301 cagtcgtcgt cacccattcg gcccgagcgc atgtcggaaa gcttgatctt cgcctcggcc
      361 gacagcagcc gcatcacgat ctcggacttg ctcatctcca gcgagaagat cacgctggcc
      421 atccgctgct tgatcgagca cgaccgcagg aaatccaaag ccagtgtgga gttgtgcgtc
      481 ggcatcatcg cacgtccggc caagtacagg tgtgcgtagt tgtcgacctg aacacaacgc
      541 accgggacgc tgccgacctg acggagcgaa tcgatacccc acgccgactg ctgccgacca
      601 cgggcaacgc tgagaaccgg tgccctcgcc gcgctgatca ggtcgacgcc cggccgcagt
      661 gccgcggtga cgctgatgcc ccggcccgtg ggccactgat gtgccgcatc cgcgacaatc
      721 gttgtgccat cggagaactc gatctcatag cacggtcgcc ctagcatcgc ctcggtggca
      781 gccaccacgc gcgtcggctg accatccgcg tccagcagct cgtcgccgac agcgacgtcg
      841 cccatggtgg tccagccggt cggcgtgggc agaggcgtgt cgagcgccag cgccttcccc
      901 acgccgggcc tggccgcgat gatgatcatc tggccggggt gcaggccgtt ggtgacgtcg
      961 tcgagttcga cgaatcccgt cggcacgccg ctggcccgcc ctccgctgga cgcgatcgcg
     1021 tcgatctcgt ccatcgtggg ctgcagcaac tgctcaagcg gcacaaaatc ttccgacaac
     1081 cgccggtcgg tgacgtcgta catctccgcc tgcgcgcggt cgaccacctc ggcgacatcg
     1141 gaaccttcgg ctccggcgta tccgtactgc acgacgcgcg tgccggcctc cacgaggcgc
     1201 cgcaacagcg ccttctccgc gacgatgtcg gcgtagtacc cggcgttggc cgccgtcggc
     1261 accgtcgaga tcagggtgtg cagatacggc gccccgccga tccgccgcaa cagcccgcgc
     1321 cggtccagtt cggcggcgac ggtcaccggg tcagctggct caccgcgagt cgacaggtcg
     1381 acgatgacgt cgtaaatgtt ctgatgcgcg ggccggtaga agtcgcctgg gcgcaacttt
     1441 tccaccacgt cggcgacagc gtccttgctg agcagcatgc cgcccagcac cgactgctcg
     1501 gcgacgatgt cttgcggcgg ctgacggccg aattcttcgt tcgacggcgg ggtccccatg
     1561 cccgaacgtc cgagatcgtc cacgacggcc attgcggcgc ccccacctcc tcccgaacgt
     1621 acgttcgcct cagctgtctc acgataagtc accgctccga caagtcgccg tttcaacgcg
     1681 ccgaggtcag ccctggacgg tccgacgcta aaacgttgct ggcgaccctg cataggggtg
     1741 ctgttgatga agctgtgcat ggcgtgtgga tatccgcttg caggcgtgtt gaagggttgt
     1801 ggataacctg tggatagcgg gcgaatttat ccccatagtc cctgctaacc ccgacctaga
     1861 aggtggtgtg agctgtagat cgcaatcggc tcggcgtgtc ttgccgggtt gcgatttcgg
     1921 gcgtgttgtg ttgcgggccg ttgaggggtg agttaacacc cggttagatt cgctgtgaac
     1981 gaggtgcggc cggaagttcg ccagtgcaca ggccgatcgg cgtcggcgac tctccaaacc
     2041 gggcaaaccg gcagttatgg cttcgccggt gaaggattga tgttcgctaa caatttgcgg
     2101 ggcgttgatt tcagggctcg tttgcaacgc gcggtggttc agtgacggcc tggtgagtca
     2161 gcggccgctc acttcgcgcg cgaattcggc tcaatcacag cgggattcgc cgggaaatcg
     2221 acgttttgca gcgaaagtcc gagaaagcac ctgcaattcg tgcgtctcgg cgcaccgaca
     2281 acggtggtag cgcctactac ccggcgacga cctcgaggga aacagtggcc tggacgtcgg
     2341 aatgcagccg cacgacgatc ggatggctgc cgagcgactt gatgtgcgtc tgcggcatct
     2401 gcaccgagcg cttgtccacg ttggggccgc cggccttctt gatcgctgac acgacgtccg
     2461 cggcggtaac cgagccgaac agcctgcctg agtcgcccgc cgttcgggcc agcagcgata
     2521 cggtgccgag tttttcgatc gcggtcttga gctcggcggc atgttcgcgg tcgcgcacgg
     2581 tcttggcatc gcgggcgcgc cggatctcgc tggcctgcct ctcggcgccg cgggtggcga
     2641 cgatcgccag cccgcgcggc agcagatagt tccggccata gccgtctttg acctctacga
     2701 ggtcgccggc cgaaccaagg tggtcgactt cggcggtgag gatcagcttc atcgtcgttc
     2761 ctgttcggta aaggctcagc gggtcgagga cgtgaacggc agcagcgcga cctcgcgcgc
     2821 gttcttcacc gcgatcgcga tgtcgcgttg atgctgcacg caattgcctg tcactcgccg
     2881 ggcacgaatc ttgccgcgct cgctgatgta tgtacgcagc agcgcggtgt ccttgtagtc
     2941 gatggcctgg ccctttttcg aacagaacac gcacttgcgc gttttgatcg gcttctcagg
     3001 tgcgcgcgtg gggcgtcgct tgttggactt cgccatggat gtttctcttt cgtattgcta
     3061 gttatggtta gaacggcggc tcgtcgtcac tgccgccgaa ggagccggag gcaggagcgc
     3121 tgccccacgg gtcgtcgggg gtggattgtt ggccaccgga ttgctttgag ccgctaccga
     3181 atccgccgct actgctgccc cggctggctt tgttcacttt cgccgtggcg tagcgcaacg
     3241 acgggccgat ctcgtcgacc tcgacctcga acaccgtgcg cttctcaccc tcgcgggttt
     3301 cgaacgagcg ctgcttgagc cgcccggtga cgatcacccg agagccgcgg gtcaggctct
     3361 ccgcgacgtt ctcagcggct tcccgccaga tgttgcaacg taagaacagc gcatcgccgt
     3421 ccttccactc cccgctctgc cggtcataaa tgcggggcgt ggacgcgacg gtgaaattcg
     3481 ccaccgcagc acccgacggg gtgaagcgca gttcggggtc ggcggtcaga tttccgacga
     3541 cggtgatcgt ggtgtcacca gccacgaggt cctcctgggc agttttgttt actcttcacg
     3601 caagcgctca tcgttgcgcg gtggttgtcg tgcgcgagcc tacgcagcag caccgacgct
     3661 cggcatacat tcggtctagt gcttgtcggt gcgcagcacc ttggtccgca acaccgactc
     3721 gttcaggctc agctgacggt cgagctcgga caccgtctcc ggcttcgccg tgacgtcgac
     3781 caccgcgtag atgccctcgg agtgcttggc gatctcgtag gccagccggc ggcgacccca
     3841 gatgtcgacc ttgtcgacct tgccgccgtc gttgcggatg acgttgagga acgcctccag
     3901 cgacggcgcc accgtgcgtt cgtcaagggt ggggtcgagg atgaccatga tttcgtatgg
     3961 acgcatagag acctcatcac ctcctatggt catgtgcggc cacggcgtat ccgtggcagg
     4021 agggtcgcct gcgtcggcaa ccgcaccaga gtagcggaag agagcttgag ctgcgaaatc
     4081 gctacttgca gggcgcggat aaagcggatg cgagctccga cgcccacgcc tcgtaggccg
     4141 ccggcgtcgg atggatgccg tcgtcgaccc actgcccgtg gtcggcctgc tgtcgataca
     4201 gcgagcgcag gtcgacgaac tcggtgccgt gtgccgatgc ttcctcaatg agaaccgtgt
     4261 cgtgggtatc acgctgagcc gacaggtcgg tgccgtagtt ctgcccttcc gtcgatcgaa
     4321 accattcgtt ttgcgccaga acgatgacgc gtgcgccgct cgccttcgcg gagtcgagga
     4381 ccgccctcac gttctgccgg gtactcatcc accgagaccc cgttaacgat gtcgttcccg
     4441 cccgcctgga aggtgatcaa cgtgggttga aagggtccga tttgcggcac ctgttcagcg
     4501 agcacttgtc ccgccgtata gccgctgaca ccgacgttca ttaactcgac ttcacaaccc
     4561 gcagaccgcc acttctccgc gagcagcgat ggaaatgcgc ctgtcgtggc agcacgctca
     4621 gtagcggtgc tggtagaact ccagcgctag ctggtgggtg gcttcggacg ccttggccag
     4681 atcacccgaa tcgaacggcg gctccgggtc gtactcgatc atcagctggc tggcttcggc
     4741 cgcctcccgg ttcacctggg ggcaccccca gcttgcgagg gggacagctc gaccaagcgc
     4801 aagcccatgt cgatgccgct cgacaccccc gccgaggtga tgatgcgctc tggcagatgc
     4861 tcgacgacgc gctgcggcac gtacgtcgcg cccagcgagt tgaacaggtc ctgcacccgc
     4921 caatgcgtgg tggcggtgag tccgttcagc agaccggccg ccgcgagcac gagcccgccg
     4981 gtgcacaccg aggtggtgta gacggtgtgc ttgtgcacct cgcgcaccca gtcgagcacg
     5041 acgtcgtcgg aaatgagcgt cctggtgccg atcccgccgg gaaagatcaa cacgtcgggc
     5101 tcggtcacct cgtcgaaggt cgcgtcacag gtcaggccga gcattccgtt gtcgctgcgg
     5161 acctcacctc gccgatgccc gacgaacacc acgtcgatcg acggaacccg ctgcagcgcg
     5221 tcgtaggggc cgacggcgtc aagggatgtg ttgcgtggga acagcgcgat cgcgatctgc
     5281 atcgaagacc tcctcgggct cgcgcggcgc tgggatgcgc ggacgcaacc acgacggtag
     5341 ccaggctggc ggcgcgtcag cggtgttctc gtagattccg ccggacgggt cgtcgacgcg
     5401 tcccccgaac cggacgaggt cacgctcggg gtggtagatc tgccacacca ccaacgcgca
     5461 caacgcgagc acggccaggt ccctcagcag caccgtgatg gtaaagggct gctcgggcag
     5521 accccgggtc tggtcgccgt acaagtagaa catgcgtggt atccagacga gcgcatcaat
     5581 cgtcatccac gccaacagaa ttcgtcgatg cggcaacgcc agcaccgcca gcggaaccag
     5641 ccacagcgaa aattgcggac tccagacctt gttcgtcaac aagaacgccg cgaccaccaa
     5701 gaacaccaac tgcgcgacac gcggccgacg cggcgcggtc agcgcgatgt aggtgatcgc
     5761 agcgcacgcg atcagaaaca gcacggcgac aacggtgttg agcaccaccg gcggttccca
     5821 gaatcccagg ttgccgtcga agcccgacca ccccgtgaac gacttgacca cgttgtacag
     5881 cgagtccatg tcgtcgccgc ggcgggcgtt gaggcggaag aactccgacc atccccgcgg
     5941 aaacagcacc agcacaggca gattcaccag aaaccaggtg agagccgctg ttccggcggt
     6001 ttgtcctacc gagcgcaacc gtccggtgcg cagacccagc gccagcaacg gcagcaacag
     6061 cagcaccggg tacagcttgg ctgcaatacc aagtcccagc aacacacccg ccagcaccgg
     6121 cttgcgccgg gcccaggcca gcatcgatcc gacggcgaaa gctgttgcca gagcgtcgaa
     6181 attcgtaaag atctggaaga tcagcaccgg ggacgcggca accagcgtcg cgtcccagat
     6241 gcgccgtccc gccaagctgg cggtggccca cacgaccccc agccacgcga gtgccagccc
     6301 gaacgcagag atgttgaaga acatcaccac ctcggcgatc acgggtatgc cgaggatctt
     6361 cgtcagcgcc gtgtaagtct tggccaccgc catcgacgcg tactggtaca acccggtcag
     6421 caccggatac tccatgtacc gaaccgcggg acggccgtcg tactgggttt tcggctttcc
     6481 ggtggaatcg ctttccagcc aactggattt gtacgggaac ttgccgaggt tcaacaactc
     6541 cgccgtgtac agcgggacgg tgtcggagta gcacagctcg taatacgcgc ggttgttgtc
     6601 ccagttcgcc acccgctcgt cagctgggcc ggtcgacgtg ctctgcaagc acgcggcttt
     6661 cgtcgaataa ccgagcgcca ggaacaccag cccaatgaga aacatcaccc gcagtggggt
     6721 gaacaacccc tggcgcccga tcagcgcgtg tcggccgact ggcccgccga tcgtctcgga
     6781 cagcgcggca cccatagcgt cggtgcgact ggggaagtcc cggtcgtcga ggctgcgccg
     6841 gtcggccgca agcggctgcg gcgaaatcac cgcagcgggt tcgggctctg cgtcgctggt
     6901 gtccgtcacg gcggcggcgg aggcccggga aggggaccct gcgcaccggc gtcgccgggc
     6961 ggaggcggag cctccggcac cgtggtgggc ggcccgatcg ggatcgtgat gccgggcgcc
     7021 acttcgatgc tcggctggat cacctcaccc ggtgtgggcg tgggcggtgt gggtgagggc
     7081 gacgacggag gaggcggcgg cggtgcggcg ggcacgccgg cgtatccgcc gatcgacgtc
     7141 ggcttgggga acgactcgtt gtcggtgccc tccagcgcac catccatggt cgccttccag
     7201 atgtctgacg gcagccctga gccatacacc ggtgatcccc acttgttttc cagcggcttg
     7261 tccccgcgga cggtgcccac ccagaccgcg gtcgccagcg acggggtgaa gcccaccatc
     7321 cacgcgtcct tgttggcgcc ggtgtcgccg agctgcgtgg tgccggtctt cgcacccgac
     7381 gggcggccgc cggcgaggtt gtggccgttg gaatacccgg cgatcggctg catcgcggag
     7441 atcacattgt tggcgaccgc ctcgtcgatg cgctgctcgc cgccgctctc cgacgtcgcg
     7501 gcgtcgaaca gcacctggcc ctcggcgttg accaccttct gcacgaagtg aggcgtgcgg
     7561 aacatgccgg acgcggcgat ggtggcgtat gccgacgcca tgtcgatagg ccgagtctga
     7621 tactggccca agacgattcc gttgctgggc ggcccaccct tgccgtcctc ggacaacgtg
     7681 tgctcgacac ccgggaaact ttcggcaatg ccggcgtcgt gcgcggcggc ggccacgtcg
     7741 gacggtccgt tcttcagctt cagcatcagc cggtagtagc tggtgttcag cgaccgcttc
     7801 agcgcctcgg cgatgttgca cgtgccgcag ccttcgcctt cgacgttggt gatctggact
     7861 ccgttgacat tcagcggcga gctgtcgatc tgggtgccca gtccgatgcc ctgttggagg
     7921 gccgcaatca acgcgaacac cttgaacgac gacccggtct gtagaccggc ttgcgcgaag
     7981 tcgaagccgt tggcttcggg gccaccgtag taagccttga cggcaccagt tttcgggtcg
     8041 atagacacta ccgcggcgcg catgtcgggg tcctggccgt cgaggtaatt gttgaccgcg
     8101 tcctcggcgg cccgttgcgc ctggggatcg atcgtggtgg tgatctgcag gccctgcgtg
     8161 ttcagcgtct gctcgtcgat gtcgaacagg tcgagcaact cgcgggtgac ctcgcgctcg
     8221 atcagcccgt cggggcccgt ggtctggctc tccatgcggg actggtccga cggcaccgtg
     8281 ggcgggaaca cctgcgccgc gcgctcactc gcgggcagcg ccccgatggt gaccatcccg
     8341 tcgagcaccc aactccaccg tttttcgctg ccttgcgggt cgacggccgg gtcgaggctg
     8401 gacggccgtt ggatcagcgc ggccagcagc gcgccctcgg aaacggtgag ttgctcgacg
     8461 ggtttgttga agtaggcctt cgacgccgcg ccgatgccgt atgcgccgcg gccgaaatag
     8521 atgatgttca gataagcttg cagcacttga tctttcgacc attcgctgga catcttcgtc
     8581 gcgatgacca actctttggc cttgcggacg acgccgccga cgccggcgcg ctgggacccc
     8641 accagcgcgt tcttgacgta ctgctgggtg atcgtcgaac cgccctgtgt gtcgccgccg
     8701 aacaggttgt tcttcaccgc ccgggcgaaa ccgctgaacg agaacccggg attggagtag
     8761 aagtcgcggt cttcggctgc cagcaccgca tcgcgcacgt gctggggcac ctggttgatg
     8821 tcgacgtcga cacggttgcc ttcgggcgga acgattttcg ccaactcggt ggtgccgtcg
     8881 ctggccagga tcgtggagac ctggttggtg cggatgtcgc cgggctgcgg cacgtcgacg
     8941 atgaggtagg ccatcccgaa ggtgacgagc ggcagcacga tcagcacggt gaccgcgatg
     9001 atcagcccgc gccgcagccg cctccagttg atcgggtgca tgggccccgg tgtcgggcgg
     9061 ggcggtgggc caccgctggg cgggccgccg ggtggcggct tcggtggcgg cgttcggtcg
     9121 agcgcggcct tgacgacgtc gacctcgtcg cgaagcggtt cggtgttcgc ggtgaccggc
     9181 ggcaggatgg tggtcggacg agtttcgggc gcggcccggt gcaccggtcc cgtgacgcgg
     9241 tcggccgccg cttcttgacg gctgtcggtg gctgaccggt cgggacgctc cccgctattc
     9301 actggccgtg cgcgcgccgg tgcgcgccgt ccgagtgcca cgggatcgcg tcgggggacg
     9361 caccgcgccg agcatgtacg acttgaccag gtgattccag ctgcaggtcc ggcatacctc
     9421 caccacgtgt accgagaatt cgtcgaaccg ggcggccagc atcaccaact cttcggccgt
     9481 gcgggctgag cccgacaccg gaccgaggtg atcgccgaac acccacgaca ccaatgtgag
     9541 ttgctccttg cgacagatcg ggcacatcac cgaactaggc ttgccgtggt atttggccgc
     9601 ccgcagcaaa tacggattgg cgtcgcagac ctccgagacg ccggtgcgtc cggaatacac
     9661 ctccgccagc agggaccggc gccgaagcgc atagtccacc acctgtcgct gcagtcgcac
     9721 gttgaccaga gtacgtccct cccccgacgg gcgaagcgga gaagcccaag ccgaccgaga
     9781 ggatgcgcgc cgtggcgtgc accgttgccg cgtcagttcg gctcgcctct tacgatcgtc
     9841 gtcgtggcga cacggcaatc agtgggcacc cgggccaagg acagcgatcg gaacgacaca
     9901 tgccaggtgc tcgacaccgc gctgtccgag ggccagttgt cgatggagga acaccgtcag
     9961 cgggtcagcg ccgcaacgag cgccgcgacg ctcggtgagt tgcaggcgct cgtcgcggat
    10021 ctgcagacct cgaacgcgcc cgtgcagctg ccgaaattga agtcgcgcaa ccaaattcag
    10081 ggtggcggcg gctggggcat gcggattgcc gtggcggcgg tgttcgtgct gttgggtgtc
    10141 ggactcggat ggggactctt cggcagcagt aactcaccgc tggccgcgga ccccggtgcc
    10201 gcgccggacg gcatcgccgc caacacgcag gcacccccgc ggctgttgca ttcggcgaac
    10261 ggcttgaacg gtctgttcgc gcaggcgcgc aagaagtggg gcgacacgat gggctacagc
    10321 ctcaccatct atcccgacta cgcgtcgatg gagcgggcgg atcccaacga cgcccgtcgc
    10381 acgcttcgct acctgtaccg aggcgggtgg gacgacaatc catcggtcag cgccatcagc
    10441 gacggggaga agttggtgga cctcgcgaag ttcgacgtga acgcgatcat cggccggctg
    10501 cggggtgcgc cacagatcct cggcatcgag caatccgagg tgaagtcgat ctacctgaac
    10561 atcgaaccga gcaaagaccc gacgacaccg gacgccgtgg tgatttctat ctacgtgtcc
    10621 accacgtacg agaagagcgg atacctgacc atcgacccgc agggcgaaat cctcagccag
    10681 aacccgccga cctgagtgcg caactgccgc caattgccgc tcaaaagccc ccgagctgcg
    10741 ggttaggctg acggcgtcgg tcgctcttcg cgcaagcgct catcgagacg agggggtcac
    10801 atggacatct ggttgcagca acaggcaaaa gctgtgctca ccgcgctgcg gcgcgcagag
    10861 cagctctacg gcggtccgac accgccgcag ggatcacgac cgttcgcgcc ccggccggat
    10921 ctggaagcgg acctgggcgg cgggttgatg tgagacaccg atgacgcagc ctgccccttc
    10981 accgtctctg ggtaccgacg aacagctcag cgggcgggcc gccgacgccg tcgccgacat
    11041 gcaaagcgag ctgcaacgcc gctactccgg attgaacggc gccgaggccc agctcgccga
    11101 ggcgctgctg actgcccagg ccgcgagcga agccggccgc gcgcggctcc agtacgtcca
    11161 gcgtcagctg atcgaggcaa tcaacaaccc ggtcaataca ctcgacaccc ccgccggcga
    11221 gcggcaattc ctgatcttct tgcgcggcaa gatcgcagag atccagcgcg tggtcgacga
    11281 cggcgcgcta accgcgcacg accaggcgga gctcgctcgc gcgctcggca gtgactattt
    11341 gactggccac ctcgtcgacg atccgtcatc ctcgccagac ccgctgccgg ctaccaaccc
    11401 tgctcggcag ccgacaccag cgacaccggc gggcgtgggt tcgttgccac cggtcagcgg
    11461 actgccgcaa gtcgcgtcgc cgtctgcatc gcctccgtca ggtctcggcg ggtccgcttc
    11521 gccactggcc ggtctagcct cgttgctcgc aggccaacag caagaggaac cgatggcggc
    11581 cgcgccgcac accaccaacg acgaacgaga cgaaggagag ggtggcgagg tcacggccac
    11641 ggaggacgac gagcgtgagc ccgacgacga cgaggcgccc gtaactcccg acgacgcacc
    11701 cccgggggac acgggcccgg ccgagacccg cccggccgcc agctaggaga acgagatggc
    11761 agaacgagac atccgcgtca caccggatgt gttgcggcag atcgcggccc gtcacgacag
    11821 gatcgccgac gagatcgcgt cggcgcgctc ggcccggcag gacatcctct ccgctgtcgc
    11881 cacctacggc ccaatcatgc acaccttcaa agacgcggtt gccgacgtgg tcgccagacg
    11941 cgacgcggcc ttcgcgaccc acgaggccga ccatcggttg gtcgcggaca aactgcgtga
    12001 cgctgcgaac cgcttcggcg agcaggagga catcaacgcg tcggagttgc agttttgagc
    12061 gatgaatcgt tcaccgcgtc gacaccggac aacaccgtat ttgtccgtgt ggccgtcgca
    12121 gggcacacct tgggcgtgca gatcgagcct ggggcgttgc ggctttctgc ccatacgctc
    12181 gccgagcgca tcatggcgtg caatgacgtc gcctatctca aaggccagct cgccatacgc
    12241 gccgagttgc agcaggccgc catccccgtc gacgacatgc caaccgagga tgacctggcc
    12301 gcggcggagc gtcggctgaa ggagttgtag accgacttga aagtgcaagt tgagacatcc
    12361 ggactcgtcg ccgcggcaga ccggctgcgg gccgccgcca cacagttgac gggcgcgggc
    12421 gtgccggttc atccgccgct ggcccgcgac cagacatccg tcgcggctgc ggcccggctt
    12481 agcgccgcgg ccacggcgct gcagtccgca gctgcgacgc acgcggctgc gttgatcgcc
    12541 acggctgagc accta
//
LOCUS       DASWBF010000536         3735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1242200,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000536 DASWBF010000000
VERSION     DASWBF010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242200"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..171)
                     /locus_tag="VF874_20125"
                     /pseudo
     CDS             complement(43..171)
                     /locus_tag="VF874_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            311..1372
                     /locus_tag="VF874_20130"
     CDS             311..1372
                     /locus_tag="VF874_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9178281.1"
                     /translation="MVEGLRLIPGGAVPVEPVVADPVVFQRECVDAFVASWRARGFSP
                     VTIDNDIGLLERTLTALGRPAWEVTSEDIDRVVGELAVAGRSASTRREYVQIFKGFHR
                     FLQTRKAAEIEAGFGVRLVCPVDEFNASRHVGDESPAVAPPPTPERVSEFFEFLKSRI
                     ATARKYGPAARDYAMFRTLYHAGLRSEESALLEIPDVHFGRGPFGKLHVRFGKAAHMS
                     GPRPRWVPMLDGLDVLLRWFLGDVRPKFPQSPVLFADESGGPLHRGTIRNRLRYLMEL
                     EGRPVAERFSPHALRRACATHNYERGVDLVAIQQLLGHWTVSSTMRYVRPSATFIEDA
                     YRRAVTLTLAELSGEDGTA"
     gene            1369..1683
                     /locus_tag="VF874_20135"
     CDS             1369..1683
                     /locus_tag="VF874_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9178282.1"
                     /translation="MKIRWRLRMAAAQREVWAGTELRRLLAETAGVEMSAASVSALFT
                     KEPSQIKLSTLIALCTALQCTPDDLFEIDTTPVERPATAPRPVPSEPKTANVRGRSMP
                     PM"
     gene            1715..3349
                     /locus_tag="VF874_20140"
     CDS             1715..3349
                     /locus_tag="VF874_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEX9178283.1"
                     /translation="MGKRQRDCVDCGAPVGYIGREHCCRCMRRLREHAAKARCPTCDR
                     DRVLVPAAGRCVLCSRCCRECGRPIRFRDEMVCRPCQKRAALAATRSTCPRCGKPGYL
                     RESTGWCGSCSRPGPPKDPPRVCMSCGKLRRHVGHGMCGRCWQRRPDRPFVRGETLAA
                     RLAEPPGWLDDFVADLAAHYCPAQACKLLTTLARLLVDDFPNHPQSVLERARVPGRSM
                     GSLARALEAFFTERGLAMATDQADRLAAGRRQRRIDTAPAGMRGAVASFADSMLYARE
                     RARRAGTRPRTDRTIESALTIVRDLARFLDSQRAKQDWALVDIADIEAFLAELPKNRA
                     RRLAVLRQFFRFARSRRLLLVDPTHGVSVKRHKGFRGRTLTIAQQRAVFRRWSANPAA
                     HPHEALVGLLAILHGASSGEVRGLRVDHIDATDRSIRLGKRPHPVPLDPVSWTALQRC
                     LDHRDRLRTDNPHVLVTRGTKADRRPASEAYMSHLLDPCGLPPKMLRATRLADLVNTI
                     DPKLVAAAFGMNPESAMAYLADHLDETRLVDLAADE"
     gene            complement(3383..>3735)
                     /locus_tag="VF874_20145"
     CDS             complement(3383..>3735)
                     /locus_tag="VF874_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9178284.1"
                     /translation="PHGACLLGPVGVGKTHLATALGHIAIRRRVPTLMMRADAMFKRL
                     KASRLDNSTEAEMRRLAQIRLLLIDDFALQPMDPTETADFYELVVARHHQASTVLTSN
                     RSPDKAHLFARTCV"
BASE COUNT          547 a         1187 c         1345 g          656 t
ORIGIN      
        1 tgtttggctc agtgggcatg atctggggtt ttcgcggtgc tgtcaacagg gtttttggcg
       61 gagccggtag gagggtccgt cgatgaccag ttcgtgcgcg gtggaggtga ggcggtcgac
      121 ggccgattcg gcgagcatcg ggtcggtcat gatggccagc cactcaactg gagctggcac
      181 atgttcgttg taaccgattt cccctgctag ctgggctttc gactgttctg cgtgtcgcgc
      241 cgatcttcaa ggcgtgatcg cgatatgtta ggtgctgcct tagccagtta ggcgggaaga
      301 aggtgtgcgg gtggtggagg gtctgcggct gattcccggt ggcgccgtcc cggtcgagcc
      361 tgttgttgcg gatcctgttg tgtttcaacg cgaatgcgtc gatgcgttcg tggcgtcgtg
      421 gcgggcgcgc gggttcagcc cggtgacgat cgacaacgac atcgggttgc tggagcggac
      481 gctgaccgcg ctcgggcggc cggcgtggga ggtcaccagc gaggacatcg atcgcgtggt
      541 tggcgagctg gcggtggcgg gccgttcggc gtcgacgcgg cgggagtacg tgcagatctt
      601 taaggggttt caccggttcc tgcagacccg caaggctgcc gagatcgagg ccgggttcgg
      661 tgtgcgcttg gtgtgcccgg tcgatgagtt caacgcctcc cggcacgtgg gtgacgagtc
      721 gccggcggtc gcgccgccac cgaccccgga gcgggtgagc gagttcttcg agttcctgaa
      781 gtcaaggatc gccaccgccc gcaagtacgg tccggccgcg cgggactacg cgatgttccg
      841 gaccctgtat cacgctggcc tgcggtcgga agagtctgcg ctgctggaga ttccggatgt
      901 gcacttcggg cgtggaccgt tcggtaagct gcacgtgcgg ttcggcaagg ccgcccacat
      961 gtccggtccg cggccgcggt gggtgccgat gctggacggg ctggacgtgt tgttgcggtg
     1021 gttcctgggc gatgtgcggc cgaagttccc gcagtcgccg gtgttgtttg ccgacgagtc
     1081 cggtggtccg ctgcatcgcg gcaccatccg caaccggctg cggtatctga tggagctgga
     1141 gggccgaccg gtcgcggagc ggttcagccc gcatgcgctg cgccgcgcgt gcgcgacaca
     1201 caactacgag cgtggcgttg atttggttgc gatccaacag cttttgggtc attggacggt
     1261 gagttcgacc atgcggtatg tgcgtccgtc ggcgacgttc atcgaggacg cgtatcgccg
     1321 tgcggtcacc ttgaccctgg ccgagctgag cggagaggac gggacggcat gaagatccgg
     1381 tggcggttgc ggatggcggc cgcccagcgt gaggtgtggg ccggaaccga gctgcggcga
     1441 ctgctggcag agacggcagg ggtggagatg tcggcggcgt cggtgtcggc gctgttcacc
     1501 aaggagccca gccagatcaa gctctcgacg ctgatcgcgt tgtgcaccgc gttgcagtgc
     1561 accccggacg acctgtttga gatcgacacc accccggtcg aacggcccgc cactgcaccg
     1621 cggccggttc cgtcggaacc gaagacagcc aacgtgcggg gccggtcgat gccgccgatg
     1681 tagattcggc gagccgccga actggttgcg gctgatgggg aaacggcaac gagactgcgt
     1741 cgactgcggc gccccggtcg gatacatcgg ccgcgagcat tgctgccggt gcatgcgccg
     1801 ccttcgtgag cacgcggcca aggcacgatg cccaacctgc gaccgggacc gagtgctggt
     1861 gccggcggcg ggccggtgcg tgctgtgctc gcgctgctgc cgggaatgcg gcaggccgat
     1921 ccgattccgc gacgagatgg tgtgtcggcc gtgccagaag cgagccgcgc tcgccgccac
     1981 gagatcgacc tgcccgcgct gcgggaagcc cggctatctg cgcgagtcga ccgggtggtg
     2041 tggctcgtgc tcacgacccg gtccgccgaa ggatccgccc cgggtctgca tgtcctgcgg
     2101 caagttgcgt cgtcacgttg ggcacggcat gtgtgggcga tgctggcagc gtcgcccgga
     2161 ccggccgttc gtgcgcggcg agacgctggc ggcccggctc gccgagccgc cgggctggct
     2221 cgacgatttc gtcgcggacc tggccgcgca ttactgcccg gcccaggcgt gcaagctgct
     2281 caccacgttg gcgcggttgc tcgtggacga tttcccgaat cacccgcaaa gcgtgctcga
     2341 gcgggcccgc gtgccgggtc gatcgatggg atccctggca cgtgccctgg aggcgttctt
     2401 caccgaacgc ggtttggcga tggccaccga ccaggccgac cggcttgccg ccgggcgacg
     2461 gcagcggcgg atcgacaccg caccggccgg aatgcgtggc gccgtcgcgt cgtttgccga
     2521 ttccatgctg tacgcgcgtg aacgcgcccg ccgtgcgggc acgcggcccc gcaccgaccg
     2581 caccatcgag tcggcgttga cgatcgtgcg cgatctggcc cgcttcctcg attctcaacg
     2641 cgccaaacag gattgggccc tggtggacat cgctgacatc gaggcgttcc tcgccgagct
     2701 accgaagaac cgggcccgac gcctggcggt gctgcgccaa ttcttccggt tcgcgcgcag
     2761 ccgccgcttg ctgctggtcg acccgacgca cggtgtgtcg gtcaagcggc acaaagggtt
     2821 tcgtggccgc accctcacca tcgcgcaaca gcgcgccgtg tttcgccgct ggagcgccaa
     2881 tcccgcggca cacccgcacg aagcgctggt cgggctcctc gccatcctgc acggcgcctc
     2941 cagcggcgaa gtgcgcggac tgcgagtcga tcacatcgac gccactgatc gctcgatccg
     3001 gctcggcaaa cggccccacc cggtgccgct ggacccggtc agctggacgg cgttgcaacg
     3061 ctgcctcgac caccgcgaca ggctgcgcac cgataacccg cacgtgctcg tcacaagggg
     3121 cacgaaggcc gatcgacgac ccgcttccga ggcctacatg agccatctgc tcgacccgtg
     3181 cgggctgccg cccaagatgc tgcgcgcgac ccggctggcc gacctggtca acacaatcga
     3241 tccgaaactc gtagcggcag ccttcggcat gaaccccgaa agcgcgatgg cctatctcgc
     3301 cgaccacctc gatgaaaccc ggctcgtcga cctcgccgcc gacgaatgac catcccggga
     3361 acctgtgcag tttccgccgc agctacacac acgttcgcgc gaacaagtgc gctttatctg
     3421 gagaccggtt cgaggtgagc acggtcgagg cctggtggtg gcgggccacg acgagctcgt
     3481 agaagtcggc ggtctcggtt gggtccatcg gctgcaaggc gaagtcgtcg atcagcagca
     3541 accggatctg ggcgagtcgg cgcatctcgg cttcggtgct gttgtcgagc cgggaggctt
     3601 tgagccgctt gaacattgcg tccgcgcgca tcatcagggt ggggacccgg cggcggatgg
     3661 cgatgtggcc gagcgcggtg gccaggtggg ttttgcccac gcccaccggg cccagaaggc
     3721 aggcgccgtg cgggc
//
LOCUS       DASWBF010000537         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1106383,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000537 DASWBF010000000
VERSION     DASWBF010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106383"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..187)
                     /locus_tag="VF874_20150"
                     /pseudo
     CDS             complement(18..187)
                     /locus_tag="VF874_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296728.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            415..705
                     /locus_tag="VF874_20155"
     CDS             415..705
                     /locus_tag="VF874_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017555187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178285.1"
                     /translation="MLVRKCLVLPGLALAASAAGMVNAPSAVAECTSSGGVTICAQGT
                     VSGPSGVPSSSGPYYPYPCEDDWLCDDGGLSVIVDPGPPGGGIDIGRPGRPR"
     gene            complement(716..1651)
                     /locus_tag="VF874_20160"
     CDS             complement(716..1651)
                     /locus_tag="VF874_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178286.1"
                     /translation="MRVLIRSSVAATVIAASTALTGAVTAVPEISPKPTVSQEVRLAA
                     SPAPGALVGAFLRNQLENCSVICPFIVQGAATVPVAAAAAPLVFVNALTTQPLLQAIG
                     TAAASVTQPAQDAAEGIITNDLNLVLPRAQRALEVAVVEAFTIGSTALTRPGQLPRAV
                     DTARNRFLTALNQPLGAPTLPTGATTPLQVAAVRAIEIGSAVAFQAGELLLLGAVRAA
                     NTAATTLSRTGSVSATIAATGASVASTVTAATTAIRSAVTAPVPAGSMAPKRPSVATQ
                     GVLRSFNMAGSELSSSATAKHNRIIKPGNRFSGQN"
     gene            1750..2235
                     /locus_tag="VF874_20165"
     CDS             1750..2235
                     /locus_tag="VF874_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX9178287.1"
                     /translation="MRPRTLATTAAAVFVTAAVGGLASRPAESLWYQRLKKPRFQPPR
                     QVFPVVWPLLYADIAAVSASTIDGLRDRGQPEKARGFAAALAANLVLNGSWSWLFFSR
                     GWLGTSAIAAGALTASSADLTRRAVATTGTRAAPLAAYPLWCAFATLLSTRIWMLNRR
                     R"
     gene            2263..>3397
                     /locus_tag="VF874_20170"
     CDS             2263..>3397
                     /locus_tag="VF874_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9178288.1"
                     /translation="MATTTSPVHTGVPSLAALFLKRVEASPDKEAFRYLDGSNWVSVS
                     WRQAADRVESLAAGLLALGVDPEQRIGIASSTRYEWILADLAVMCAGAATTTVYANTN
                     AADTAYILGDSESRVVFAEDADQLAKLTERRADLPNVAKVVVFDGAGDGDWVMTLDDL
                     ADLGAKHLGDHPESVRDAVDNITSERLATLIYTSGTTGRPKGVRLRHEAWVYAGMAVS
                     ELRVLGEDDLQLLWLPLAHAFGKVLISAQLACGFASAVDGRVDKIVENTGVVKPTFMA
                     AAPRIFEKAHSRVVMTQQEQGGVKEKLFQRAFAVGKAVDRRRRANQSVSPVMTLQHKL
                     FDRLVFSKVRQVFGGRIRFFVSGSAPLNREIAEWFHAAGLLILE"
BASE COUNT          527 a         1106 c         1170 g          594 t
ORIGIN      
        1 ccgcagggat cggcaactca cccgggacgc agatcgggca aggcccgaat gccgaagtgg
       61 tgtttcagga ctcgccgtgc ggagtgcatg ccgcacatgc catgaacgcc cgggccggga
      121 gtgtggacgc cgaacacaga tacacattgt cgatcggcgt gcggtagggg ttccacttca
      181 gcaccggcca ccgcgggata actcaacaac accgtcggag tcttgctgtg acgtcctgcc
      241 gcggtgtccc gttctgcctg cgccttcgcg cctggctccc accgatgtgc accgaaccca
      301 ccacgacgac gtcgcgttgg ccgcccatgc tggtcatctt cgccgactac cccgcttgcc
      361 gattagttgc accattgtcc ggcacttcga tgatcggcgc attacgcttc gcccatgctc
      421 gtacgtaagt gtcttgtgct accgggattg gccctcgccg cgagtgctgc aggaatggtg
      481 aacgcgccgt cggccgttgc cgagtgcacc tccagcggcg gagtcacgat atgcgcgcaa
      541 ggtaccgtgt caggcccgtc aggggtgccc tcgtcgtccg gcccgtacta cccgtacccg
      601 tgtgaggacg actggctctg cgacgacggc ggcctttcag tcatcgtcga cccgggtccg
      661 cctggcggcg gcatcgacat cggcaggccc ggtcggcccc gctgactttc agcggttagt
      721 tctgtccgct gaatcggttg cccggcttga tgattcggtt gtgcttggcg gtggcgctgc
      781 ttgacaactc ggatccggcc atattgaaac tgcgcaacac gccttgagtc gccacgctcg
      841 gccgcttcgg ggccatcgac cccgctggga cgggtgcggt caccgcagag cggatcgcgg
      901 ttgtcgcggc cgtgaccgtc gacgcgaccg atgcgcccgt cgcggcgatc gtggccgaaa
      961 cactcccggt gcgcgacagc gtcgtggccg cagtgttggc cgcccgaacc gcaccgagca
     1021 gcagaagttc tccggcctgg aacgcgaccg ccgagccgat ctcgatcgcg cggacagccg
     1081 cgacctgcag cggggtcgtc gctccggtcg gcagtgtcgg cgccccgagc ggttgattga
     1141 gggcggtgag gaaccgattg cgcgcggtgt cgaccgcccg cggaagctgg ccgggcctgg
     1201 tgagcgcggt cgagccgatg gtgaacgcct ccaccaccgc aacctccagg gcccgctgtg
     1261 ctcgcggcaa taccaggttc aggtcgttgg tgatgatgcc ttccgcggcg tcctgtgctg
     1321 gttgggtcac cgatgcggcc gccgtgccga tcgcctgcaa cagcggttgt gtcgttagcg
     1381 cgttgacgaa gacaagcgga gccgctgctg cggcgacggg aaccgtggcg gcgccctgga
     1441 cgatgaacgg gcatatgaca ctgcagtttt cgagttggtt acgcaggaac gcaccgacaa
     1501 gcgcacccgg cgccggtgac gcggcgagcc gcacctcctg gctaactgtc ggtttcggcg
     1561 aaatctccgg caccgcggtc acggcgccgg tcagagcagt gctggccgcg atgacggtcg
     1621 cggcaactga cgaacggatg agcacccgca tagcgctccc cctcgatcga tgtgagcggc
     1681 tataccctta cccacgagcc gatcgtcggc ccggttcaat gcgacggttt gttgcgagaa
     1741 aggcaccaca tgcgcccgag aactcttgcg acgacggcgg cggcggtctt cgtcaccgcg
     1801 gccgtcggtg gactggccag ccggcccgcc gagtcgctgt ggtaccagcg gttgaagaag
     1861 ccgcgttttc agccgccgcg gcaggtcttt ccggtggtgt ggccgcttct gtatgccgac
     1921 atcgccgcgg tgtccgcctc gacgatcgac ggcctgcggg atcgaggcca gcccgaaaag
     1981 gcccgcggat tcgccgcggc cctcgcagcg aaccttgtgc tgaacggcag ctggagctgg
     2041 ctgttcttca gtagaggctg gctgggcacc tcggcgatcg cggctggggc gctgaccgcc
     2101 agcagcgccg atctgacccg gcgggctgtc gcgaccaccg gtacccgcgc cgcgccgctc
     2161 gcggcctatc cgctgtggtg tgcattcgcg acattgctgt ccactcgcat ctggatgctg
     2221 aaccggcggc gctgaccggg ctagtccggc cacaatgggg gcatggctac caccacatca
     2281 cccgtgcaca ccggagtgcc cagtctggcg gcgctgttct tgaagcgagt cgaggcctcg
     2341 cccgacaaag aggcgttccg ctatctggac ggaagcaact gggtgtcggt cagctggcga
     2401 caggcggccg accgggtgga gtcgttggcc gccgggctgc tcgcgctcgg cgtcgaccct
     2461 gagcagcgta tcgggatcgc gtcgagcacc aggtacgaat ggatcctcgc cgatctcgcc
     2521 gtcatgtgcg ccggggcggc gaccacgacg gtctacgcga acaccaacgc cgccgacacc
     2581 gcctacattc tgggcgactc ggagtcgcgg gtggtgttcg ccgaggacgc cgaccagctg
     2641 gcgaagctga ccgagcggcg tgccgacttg cccaacgtcg cgaaggtcgt ggtgttcgac
     2701 ggcgccggcg acggcgactg ggtgatgacg ctggacgacc tggcagatct cggggcgaag
     2761 catctcggcg atcatccgga gtcggtccgc gacgccgtcg acaacatcac ctccgaacgc
     2821 ttggcgacct tgatctacac ctccggaacg accggccgac ccaagggtgt gcggctgcgg
     2881 cacgaggcgt gggtgtacgc ggggatggcc gtatccgaat tgcgtgtgct cggcgaggac
     2941 gacttgcagc tgctgtggct gccactggcc cacgcgttcg gcaaggtgct gatctcggct
     3001 cagctggcgt gcgggttcgc cagcgcggtc gacgggcgtg tcgacaagat cgtggaaaac
     3061 accggagtgg tcaaaccgac cttcatggcg gccgccccgc gcattttcga gaaggcgcac
     3121 tcccgcgtcg tgatgactca gcaggagcag ggcggtgtga aggagaagct gttccagagg
     3181 gccttcgccg tgggcaaggc ggtcgatcgt cgccggcggg cgaatcagtc ggtttcaccc
     3241 gtaatgacct tgcagcacaa gctgtttgac cgtctggtgt tcagcaaggt gcggcaggtg
     3301 ttcggcggcc ggatccgatt tttcgtctcc ggctccgcgc cgctgaaccg ggagatcgcg
     3361 gagtggttcc acgccgctgg gctgttgatt ctggagg
//
LOCUS       DASWBF010000538         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1106489,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000538 DASWBF010000000
VERSION     DASWBF010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106489"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..2045
                     /locus_tag="VF874_20175"
     CDS             348..2045
                     /locus_tag="VF874_20175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunits gamma/tau"
                     /protein_id="HEX9178289.1"
                     /translation="MALYRKYRPATFAEVVGQEHVTEPLSTALKAGRINHAYLFSGPR
                     GCGKTSSARILARSLNCVQGPTATPCGECESCVALAPNGPGSIDVVELDAASHGGVDD
                     TRELRDRAFYAPAQSRYRIFIVDEAHMVTTAGFNALLKIVEEPPEHLIFVFATTEPDK
                     VLPTIRSRTHHYPFRLLTPRTMRALIERIVSQEGVEIDDAVYPLVIRAGGGSPRDTLS
                     VLDQLLAGAESDRITYPRALALLGATDVALIDDAVDALVAGDAAALFGAVESVVDAGH
                     DPRRFATELLERFRDLIVLQAVPDAVSRGVVDAPGDVLERMRDQAARIGTATLTRYAE
                     VVHAGLGEMRGATAPRLLLEVVCARLLLPSASDTESALLQRVERIETRLDMSVPEAAA
                     QPVPAAKPTAPRKPESPLPKPEPTPPPEPPTEIGLPDAAAVRRQWSTVMDKVRERSRT
                     LGAMLADAVVSTVDGDTLVLSHQSGNLAKRLGEQRNSDVVREALKDALGVDWKVRSEL
                     AAPGSDPKPAEDAQRVEEDSMLAEAGQESPADATRRDPEEVALEILQNELGARRIDS"
     gene            complement(2042..>2795)
                     /locus_tag="VF874_20180"
     CDS             complement(2042..>2795)
                     /locus_tag="VF874_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9178290.1"
                     /translation="NVYPIVKRYFWSEVRGTELIPPGAALGVSNHSGGMFTSGGMFTP
                     DPLILGSDFYERFGYERPMYTLAHNTVMMGPLEDNLLRLGVIHATRENASQALESGAV
                     VLVFPGGDYDSYRPTSAENVVDFNGRTGYVRTAIEACVPIVPTVSIGAQESQLFLTRG
                     TGLAKRLGLDKRLRLKILPISVGFPFGVSVILPPNLPLPTKIVTQVLEPIDIAAQFGE
                     NPDVKDVDSQVRSVMQVALDELAQERCFPILG"
BASE COUNT          468 a          937 c          927 g          463 t
ORIGIN      
        1 gcaggccgtt aacggcctgg ccacctgcgc gctgctggcg gccaccgagt cgctgctgct
       61 gcggggctag cgaaggcgct aagtcccagc cgccacaatt gtcaccgccc ggggtcatac
      121 ttcgacttgc ctgcctctgc gcgacagccc ctgacctgct tgttcgctat gagtttgtcg
      181 ctgatcgctt tgtccgcgtc gtgaccagga cgtgggcaaa cgcgttttgt cgctcagatg
      241 cgggcatttc gaacactgcc aggccgagag cctgaagggg cctctgtggt tggtgacgag
      301 agaacgcgcc tcggacggcg tcacaccccc tcggtagcct gctgtccgtg gccctttacc
      361 gcaagtaccg tccggcgact ttcgccgagg tggtgggcca agagcacgtc accgaaccgc
      421 tgtccacggc gctgaaggcg ggacggatca accacgccta cctgttctcc gggccccgcg
      481 gatgcggcaa gacgtcgtcg gcgcgcatcc tggcccgctc gctcaactgt gtgcaggggc
      541 ccacggccac cccgtgcggc gaatgcgagt cgtgtgtggc gctggcgccc aacggtcccg
      601 gcagcatcga cgtcgtcgaa cttgacgcgg ccagccacgg cggcgtcgac gacacccgcg
      661 aactgcgcga ccgcgcgttc tacgcgcctg cgcagtcgcg gtaccgcatc ttcatcgtcg
      721 acgaggcgca catggtcacc acggcaggct tcaacgcgct attgaagatc gtcgaggagc
      781 cgcccgaaca tctgatcttc gtgttcgcca ccaccgagcc cgacaaggtg ctgccgacca
      841 tccggtcgcg cacccatcac tacccgttcc ggctgctgac cccgcgaact atgcgggcgc
      901 tgatcgagcg gatcgtttcg caggagggcg tcgagatcga cgacgcggtc tacccgttgg
      961 tgatccgcgc cggcggcgga tcaccccgcg acacgctgtc ggtgctggac cagctgttgg
     1021 ccggtgccga aagcgaccgc atcacgtacc cccgggcgct ggccctgctc ggcgccaccg
     1081 acgtcgcgct gatcgacgac gcggtcgacg cgctggtggc gggcgacgcg gccgcgctgt
     1141 tcggcgcggt cgagtctgtc gtcgacgccg ggcacgatcc gcggcggttc gctaccgagc
     1201 ttctggagcg gttccgcgat ctgatcgtgc tgcaggccgt ccccgacgcc gtgtcccgcg
     1261 gcgtggtcga cgcgcccggc gatgtgctgg aacgcatgcg cgaccaggcg gcgcggatcg
     1321 ggaccgcgac gctgacccgc tacgccgagg tggtgcacgc cgggctgggt gagatgcgcg
     1381 gcgccaccgc gccgcggctg ctgttggagg tggtgtgcgc gcggctgctg ttgccctcgg
     1441 ccagcgacac ggaatcggct ctgctgcagc gggttgagcg catcgagacg aggctggaca
     1501 tgtcggtgcc cgaggcggct gcgcaacctg tgccggctgc caaaccgact gcgccacgca
     1561 agccggagtc gccgctgcca aagcccgagc ccactccgcc gcccgaacct ccaacggaga
     1621 tcggccttcc cgatgccgcc gctgtgagac gacagtggtc cactgtgatg gacaaggtcc
     1681 gcgaacggag tcgcaccctc ggcgcaatgc tggccgacgc ggtggtgtcg acggtcgacg
     1741 gcgacacgct ggtgctgtcg catcaatccg gcaacctcgc caaacgtctt ggcgagcaac
     1801 gtaattccga cgtcgtccgc gaggcgctca aggacgctct gggcgtggac tggaaggtgc
     1861 ggtccgaact ggcggcgccc ggatcggatc cgaagccagc cgaggacgcg cagcgcgtgg
     1921 aggaggacag catgctggcg gaggcggggc aggagagccc cgccgacgcg acgcgccgcg
     1981 atcccgagga agtggcgctg gaaatcctgc agaacgagtt gggcgcccgc cggatcgact
     2041 cttagcccag gattggaaag catcgttcct gcgccagttc gtcgagagcg acctgcatca
     2101 ccgaacgaac ttggctgtca acatctttca cgtcggggtt ctcgccgaac tgcgcggcga
     2161 tgtcaatcgg ttccagcacc tgcgtaacga tcttggtggg tagcggcagg ttgggtggca
     2221 ggatgacact tacgccaaac ggaaacccaa cgctgatcgg caggatcttc aaccgcaacc
     2281 gcttgtcgag gcccagccgt tttgcgagcc ctgtgccgcg ggtcagaaac agttggctct
     2341 cctgggcacc gatcgacacc gtcggcacta tcggaacaca cgcttctatc gcggtcctga
     2401 cgtagcctgt gcggccattg aagtcgacga cgttttcggc cgaggtcggg cggtaggagt
     2461 catagtcccc accgggaaac accagcacga cggctccgga ttccaatgcc tggctggcat
     2521 tctcgcgcgt cgcgtgaatg acacctaacc gaagcaggtt gtcttcgagc ggtcccatca
     2581 tcaccgtgtt gtgtgccaac gtgtacatgg gacgctcgta gccgaacctc tcgtagaagt
     2641 cagaaccgag aatcagcgga tccggcgtga acatcccgcc agacgtgaac atcccgccag
     2701 agtggttgga cacccccaac gccgcgcccg gcgggatcag ctcggtcccg cgcacctccg
     2761 accagaagta gcgcttcacg atcggataga cgttg
//
LOCUS       DASWBF010000539         8014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_843447,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000539 DASWBF010000000
VERSION     DASWBF010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843447"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="VF874_20185"
     CDS             complement(<1..1532)
                     /locus_tag="VF874_20185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9178291.1"
                     /translation="MLIKRAATYREIYDAFRWQVPARFNMAVACCDRWAADPARVALI
                     HEQPDGEVRRYSFAELQGLSNRCANMLVGLGLARGDRVLLLLGQRPETAILHLGAWRA
                     GMISVLCSVLFGADAVQYRVQTPGAKLVITDEANLATALEAAGEARVLCVDGACESAD
                     DFWALLREGADTFTTVDTAADDPAFICFTSGTTGPAKGALHAHRTLLGHVPCIEMQHD
                     FFPQRGDLQWGPLDWAWIAGLMDLLMPSWFHGMPVLAFRSIGFDPEQAYHMMAKHRVT
                     NALLVPTMIRLMKQVPDPLARFDLNLRSLVSGGEAVGKEIIEWTQETLNVRINEVFGQ
                     TECNLVLGHNAMLMQPKPGSLGQPIPGHVGAVVDDEGNELPPGEVGHIAFKRPDPVML
                     LEYWNNPQATKDKFVGNWLLTGDLGQRDEDGYFWYHGRADDVITSAGYRIGPGEIEEA
                     LLRHPAVRLAAAIGAPDPARTESIKAFLVLAEGQRPSKALEDEIREFVRTRLARHEYP
                     RA"
     gene            complement(1586..2373)
                     /locus_tag="VF874_20190"
                     /pseudo
     CDS             complement(1586..2373)
                     /locus_tag="VF874_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
     gene            complement(2397..3365)
                     /locus_tag="VF874_20195"
     CDS             complement(2397..3365)
                     /locus_tag="VF874_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178292.1"
                     /translation="MRARHCSLIALLLVVVAAACAAAPPDRRSQVDGLTQRIGAMPGV
                     MQAGNTFNDSAAQGPAYFELDVNVADDITGDQLAAITSAYLGDLAAQDYRGYKAQLDV
                     RTDANVFLVDGRTVGNRDQILAQARSWVALQQQFPGSTVGLRATTNAVPNSGNLQLPD
                     ATDYAAVAAAVDKLGATFGELAAGDWTVSAGKQHPAEIRTSRRLPSQLEMELWRTLNS
                     DQSIPHADVFTINGAVTAPLSVSEKMPADDPGMALRLAQQHLPIVARLPAPVLYSATN
                     QYQGRIDFHGQATGPVTVLIGGCMKRNYRPSPAEQALIDTYENCRR"
     gene            complement(3590..4486)
                     /locus_tag="VF874_20200"
     CDS             complement(3590..4486)
                     /locus_tag="VF874_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9178293.1"
                     /translation="MDDDLAGLDEFALLPENAEQAGVDGPLQPVSRLDHGAVSALKWG
                     ATPPRVVFLHGGGQNAHTWDTVIVGLKEPALAVDLPGHGRSGWRDDGDYGPIQNAETL
                     QPALREWAPAADLFVGHSLGGLTAIRLATSAPDLVRRLVLVDVTPSAPQRHVEMTDAQ
                     KGTVALIQGDRVFESFAAMVDVTVAAAPHRDRKSLRRGVFHNSKQLDDGTWTWRYDSF
                     RKGDGYSDFQGLWDDVPALTIPATLVRGANSAFVNDDDADEFARTAPGFVEVQVVADS
                     GHSVQSDQPRALIDILRAVLDG"
     gene            complement(4500..5588)
                     /locus_tag="VF874_20205"
     CDS             complement(4500..5588)
                     /locus_tag="VF874_20205"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX9178294.1"
                     /translation="MSGKKTAGPEVRVAIVGVGNCASSLVQGVQYYQDADENSTVPGL
                     MHVRFGQYHVRDVKFVAAFDVDAKKVGFDLSEAIFASENNTIKIADVPPTDVMVQRGP
                     TLDGIGKYYADTIELSDAEPVDVVKVLRDAEVDVLVSYLPVGSDEADKFYAQCAIDAG
                     VAFVNALPVFIASDPVWAKKFADAGVPIVGDDIKSQVGATITHRVLAKLFEDRGVQLD
                     RTMQLNVGGNMDFLNMLERERLESKKISKTQAVTSNLQREFKTKNVHIGPSDHVGWLE
                     DRKWAYVRLEGRAFGDVPLNLEYKLEVWDSPNSAGVIIDAVRAAKIALDRGLGGPIIP
                     ASAYLMKSPPEQMPDDVARTKLEDFIQS"
     gene            complement(5641..6177)
                     /locus_tag="VF874_20210"
     CDS             complement(5641..6177)
                     /locus_tag="VF874_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9178295.1"
                     /translation="MLELAILGLLLESPMHGYELRKRLTGLLGAFRAFSYGSLYPALR
                     RMQLDGLIVEDAAPAGAPVRRARRVYRLTEPGRQRFTELVADTGPQNYTDDGFGVHLA
                     FFNRTPAEARMRILEGRRRQVEERREGLREALARASSSFDRYTRQLHQLGLESSEREV
                     KWLNELIAAERVAQGHAE"
     gene            complement(6303..6782)
                     /locus_tag="VF874_20215"
     CDS             complement(6303..6782)
                     /locus_tag="VF874_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178296.1"
                     /translation="MSVDEFVRHVHEALTRADSLFGSAPADGGLVSHQLADAAEVLRH
                     DAAADMTGAAVAGYRTFAQDLASSLDRLADADAALNRTLQDAASAENAAAAESRSTVA
                     AAVTHVDGLASTAATPGGQRALIMALHSEVGRQQNLVRRHQQQAVELGEQMRLLSYD"
     gene            complement(6793..7830)
                     /locus_tag="VF874_20220"
     CDS             complement(6793..7830)
                     /locus_tag="VF874_20220"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9178297.1"
                     /translation="MDVPAPAPTLTVTCAGAAYRIPLQNAPATIGRAPDADVPVADPR
                     VSPAHVRLELDRAGWRAVDADTRTGMFVDGVRHTAVDISDGLTIHVGNPEGPAVEFGF
                     ADDDPDSLDDDEHLDSGIARAGAAVAARRRELDITQRTLAKYRIMNAGALIAFEKGRS
                     WPRERTRAKLEEVLQWPPGTIANIRNGGAVPGEGGEPERGTDEAPLIVGAVDVAIKTF
                     ATAIDALPDPSSPEFAQRAAVVLADLRQLEAVVARAARQSRGLPAIVMALSAVRNRYQ
                     DLMFRAAAAPDATLGQRLFAARRRANLSAAETGNAVGLSAQLIEAVEAGQPVTADDAA
                     AITALLTQLGG"
BASE COUNT         1239 a         2717 c         2691 g         1367 t
ORIGIN      
        1 atcgcgcgcg ggtattcgtg acgcgcgagc cgcgtcctca cgaactcgcg gatctcgtct
       61 tccagcgcct tgctcggccg ctggccttcg gcgagcacga ggaaagcctt gatgctctcg
      121 gtgcgcgccg gatccggcgc gccgattgcc gcggcaaggc gcactgccgg atgccgcagc
      181 agcgcctcct cgatctcgcc cggcccgatg cggtaaccgg ccgaagtgat gacgtcgtcg
      241 gcgcgaccgt gataccagaa gtagccgtcc tcgtcgcgct gacccaggtc gcccgtcagc
      301 agccaattgc cgacgaactt gtccttggtt gcttgcgggt tgttccagta ctcgagcagc
      361 atgaccgggt ccggtcgctt gaaggcgatg tggccgacct ctccaggcgg caactcattg
      421 ccttcgtcat cgacaacggc accgacgtga cccgggatcg gctggccgag cgagccgggc
      481 ttcggctgca tcagcatcgc gttgtggccg agcacgaggt tgcattcggt ctggccgaac
      541 acctcgttga tgcgaacgtt cagcgtctcc tgtgtccact cgatgatctc cttgcccacg
      601 gcctcgccgc ctgataccag cgaacgcaga ttgaggtcga aacgcgcgag cggatctggg
      661 acctgcttca tcaaccggat catcgtcggc accagcagcg cgttcgtgac ccggtgtttg
      721 gccatcatgt gataggcctg ctcagggtcg aagccgatgg agcggaacgc aagcaccggc
      781 atgccgtgga accaggacgg catcagcaga tccatcagcc ccgcgatcca ggcccagtcg
      841 agtgggcccc attgcaggtc gccacgctga ggaaagaaat cgtgttgcat ctcgatgcag
      901 ggcacgtggc cgagcaaggt gcgatgggcg tgcagcgcgc ccttggccgg gccggtcgtg
      961 cccgaggtga agcagatgaa ggccggatcg tcggcggcgg tgtcgacggt cgtgaatgta
     1021 tccgcgccct cgcgcaacag cgcccagaaa tcgtccgcgc tctcgcacgc gccatcgacg
     1081 cagagcacgc gcgcctcgcc cgccgcctcg agcgccgtcg cgaggttggc ttcgtcggta
     1141 atcactagct tggcgccagg agtctgcacc cggtactgca cagcatcggc gccgaacagc
     1201 acggagcaca gcacgctgat catgccggcc cgccaggcac ccagatgcag gatcgcggtt
     1261 tccggccgct gaccgagcag cagcagcacc cggtcgcccc gcgcaaggcc gaggccgacg
     1321 agcatgttgg cgcagcggtt ggagaggcct tgcagctcgg cgaagctgta gcggcgtact
     1381 tccccgtccg gctgttcgtg gatcagcgcg acgcgggcgg gatcggccgc ccagcggtcg
     1441 cagcaggcca ccgccatgtt gaaccgcgcc ggtacctgcc agcgaaacgc atcgtagatc
     1501 tcgcgatagg tggcggcccg cttgatgagc atgattcctc cggggtcctg ggaggaagat
     1561 agctcaattc acgacgagca ttttctcaat tcgaacggtg atccctccac ccgcacagtg
     1621 cctcggcgct cgtcaggtcg tagtcggggc cgttcgcgcc gaccgtcaag aacgtcaccc
     1681 cgaggtccgc cagcgcgtcg gcctccgcga gcaagccatc accggtgccc tgcacgcccg
     1741 cggagcgttc gatcgtgtcg gggtcgcggc ccaccgcggc gcagtgctcg gcgagggtct
     1801 cggccttgcg gggatacgtc tcgcggtcga cgaacccgtg ccaggtgtcg gcgtgctcgg
     1861 ccaccagccg cagcgtcttc cgctcgccct gcccgccgat caggatcggg atgtgccgcg
     1921 tcgggggcgg gttcagtttc gggaaacgcc gtttgatgcg cggcagcgcg tcggcgaggt
     1981 cgtccagccg gctgcagacg gtgccgaact cgtagccgta ctcgtcgtaa tcctgctgct
     2041 tccagccgga gccgatcccg aggatgagcc ggccgccgga catgtggtcg accgtgcggg
     2101 ccatgtcggc gagcagctcc ggttgcggta tgagttgcac gtcaccagcg cgccgatctc
     2161 gatgcgtgag gtctgctcgg cccaggcgcc cagcatcgtc cagcattcaa agtgcggccc
     2221 gtccgggtcg ccgtagagcg ggtagaagtg gtcccagttg aaggcgacgt cgacgccgac
     2281 gtcctcgcag cggcggacgg catcgcggat gtcggcgtag tgcggcgcgt gctgcggttg
     2341 cagctgcacg ccgatgcgta tcggaaaggg catgcggctc accgtatcgc gggacgtcag
     2401 cggcggcagt tctcgtatgt gtcgatcaac gcctgttcgg cgggcgacgg ccggtaattc
     2461 cgcttcatgc agccaccaat caacaccgtg accggcccgg tggcctgccc gtgaaagtcg
     2521 atgcgcccct ggtactgatt ggtcgccgag tacaacacgg gcgcgggcag ccgggcgacg
     2581 atcggcagat gctgctgggc gagccgcagc gccatgccgg gatcgtcggc cggcatcttc
     2641 tcggacaccg acagcggcgc ggtcactgcg ccgttgatcg tgaacacgtc ggcgtgtggg
     2701 atcgactggt cggaattcag cgtgcgccac agctccatct ccagctgact cggcagccgt
     2761 cgggaggtac ggatctccgc ggggtgttgc ttgccggcgc tcacggtcca gtcgccggcg
     2821 gccagctcac cgaaagtggc gccgagtttg tcgacggccg ccgcgaccgc ggcgtagtcc
     2881 gtggcgtcgg gtagttgaag gttcccggaa ttcggtacgg cgttcgtcgt ggcgcgcaac
     2941 ccgaccgtgc tgccggggaa ctgctgctgc agcgccaccc aactgcgcgc ctgcgccagg
     3001 atctggtcgc ggtttcccac ggtgcggccg tcgaccaaga acacgttcgc gtcggttcgg
     3061 acgtcgagtt gggctttgta gccgcggtag tcctgcgccg ccaggtcgcc gagataggcc
     3121 gaggtgatcg ccgcgagttg gtcgccggtg atgtcgtcgg ccacgttgac gtcaagctcg
     3181 aagtaggcgg gtccctgcgc ggcgctgtcg ttgaacgtgt tgccggcctg cattaccccc
     3241 ggcatcgcgc cgatccgctg ggtgagcccg tcgacctgcg atcgtcgatc gggaggcgcg
     3301 gcggcgcagg ccgccgcgac aaccacgagc agcagagcga tgaggctgca gtggcgtgcg
     3361 cgcatcagcc gattctgcca acgatcagcg acaacttgtg actgcgacgt tggtcggggg
     3421 ctgtcgctgg gaggtgggca aacacatgcc tccccatcaa agcgaaagct gtgactcatg
     3481 cgaccagcgt gacaactgtt gtcacttcag ccccacatgc caccgggcag gcccggagtc
     3541 gaaccgcccg ccttcgagcg acaaatttcc gccatttcgc aagtggcggt cagccgtcca
     3601 gcaccgcacg caggatgtcg atcagtgccc ggggctggtc gctctgcacc gaatgcccgg
     3661 agtcggcgac cacctgcacc tcgacgaatc ccggtgccgt gcgggcgaat tcgtcggcgt
     3721 cgtcgtcgtt gacgaacgcc gagttggcgc cacgcaccag tgtggccggt atggtgagcg
     3781 cgggcacgtc gtcccacagg ccctgaaaat ccgagtagcc gtcgcccttt cgaaaggaat
     3841 cgtagcgcca cgtccacgtg ccgtcgtcga gctgcttgga gttgtgaaac acaccgcgtc
     3901 gcaacgactt ccggtcacga tgaggagcgg cggccaccgt cacgtccacc atcgcggcga
     3961 agctctcaaa gacgcggtcg ccctgtatca gcgcgacggt tcccttctgc gcatcggtca
     4021 tctcgacgtg tcgttgcggc gcggacgggg tgacgtcgac gagcacgagc cgccgcacga
     4081 gatcgggggc ggatgtcgcc agccggatcg ccgtcagccc gcccagtgaa tgcccgacga
     4141 aaaggtctgc ggcgggggcc cattcgcgca gcgcgggttg tagcgtctca gcgttctgga
     4201 tcggtccgta gtcgccgtcg tcgcgccagc ccgaccggcc gtggccgggt agatcgaccg
     4261 cgagcgccgg ctctttcagc ccgacgatca cagtgtccca ggtgtgtgcg ttctggccgc
     4321 cgccgtgcag gaacaccact cgcggcgggg tggcgcccca tttgagggcg ctcaccgccc
     4381 cgtggtcgag ccggcttacc ggctgcagcg ggccgtcgac accggcttgc tcggcgttct
     4441 cgggcagcag cgcgaactcg tcgaggcctg ccaggtcgtc gtccacgaga acggaggggt
     4501 cagctctgaa tgaagtcctc gagcttggtg cgggccacgt cgtcgggcat ctgctccggc
     4561 gggctcttca tcaggtaggc cgacgccggg atgatcggac cgcccagccc gcggtccaac
     4621 gcgatcttcg ccgcgcgcac ggcgtcgatg atcacgccgg ccgagttcgg cgagtcccac
     4681 acttcgagct tgtactccag gttcagcggc acgtcaccga acgcccggcc ttccagccgc
     4741 acgtaggccc acttgcggtc ctcgagccag ccgacgtggt cggacgggcc gatgtggacg
     4801 ttcttggtct tgaattcccg ctgcaggttg ctggtgacgg cctgggtctt ggagatcttc
     4861 ttgctctcca gccgctcccg ctcgagcatg ttgaggaagt ccatgttgcc gccgacgttg
     4921 agctgcatag tgcggtcgag ctgcacgccg cggtcctcga acagcttggc cagcacgcgg
     4981 tgcgtgatcg tcgcgccgac ctggctcttg atgtcgtcgc cgacgatcgg cacgcccgcg
     5041 tcggcgaact tcttcgccca caccggatcg gaggcgatga acaccggcag cgcgttgacg
     5101 aacgccacgc cggcgtcgat cgcgcactgc gcgtagaact tgtcggcctc gtcgctaccg
     5161 accggcagat acgacaccag cacgtcgacc tcggcgtcgc gcagcacctt gaccacgtcc
     5221 accggctcgg catccgacag ctcgatcgtg tcggcgtagt acttgccgat gccgtccagc
     5281 gtcggtccgc gctgcaccat cacgtccgtc ggtggcacgt cggcgatctt gatcgtgttg
     5341 ttctccgacg cgaagatcgc ctcggacagg tcgaagccca ccttcttggc gtcaacgtcg
     5401 aacgcggcga cgaacttcac gtcgcgcacg tggtactggc cgaagcgcac atgcatcaga
     5461 cctggcaccg tcgaattctc gtcggcgtcc tggtagtact gcacgccctg caccagcgag
     5521 gacgcgcagt taccgacgcc gacgatcgcg acacgcacct ccggcccggc ggtcttcttg
     5581 ccactcatgt gcggttctcc ttacctctgc caagaacttg catgtttgcg gtgcgcgggg
     5641 ttactcggcg tgaccctgtg caacccgttc cgcggcgatc aattcgttga gccatttcac
     5701 ttcccgctcg ctggactcca ggcccagctg gtgcagctgg cgggtgtagc ggtcaaacga
     5761 gctactggcc cgcgccaagg cttcgcgcag accttcccgc cgttcctcca cctgacggcg
     5821 ccggccctcc aggatccgca tccgcgcctc ggccggagtg cggttgaaga acgccagatg
     5881 aacgccgaaa ccatcgtcgg tgtagttttg cggaccggtg tcggcgacga gctcggtgaa
     5941 ccgttgccgg cccggctcgg tgagtcggta tacccggcga gcgcgccgca ccggagcgcc
     6001 ggcaggcgcc gcgtcctcga cgatcagccc gtcgagctgc attcggcgca gcgccgggta
     6061 cagcgaaccg taggagaagg cccggaaagc accgagtaaa cccgtcaagc gtttccggag
     6121 ttcgtagccg tgcatcggcg actcgagcag aagtcccagg atggcaagct ccagcaccga
     6181 gtcacctcct tagcttggcc gctacgacgc atcgacacct cgcgccatcg tatcgcgccg
     6241 atatattcgc cgccaggtga gggttcaccc gctgttcacc ggcccgccgc gatcgcgatg
     6301 cgtcagtcgt acgacagcag ccgcatctgc tcgcccaact cgaccgcctg ctgttggtgt
     6361 cgtcgcacga ggttctgctg acggccgacc tccgagtgca gcgccatgat gagcgcccgc
     6421 tgcccaccgg gagtcgcggc agtgctcgcc aagccgtcca cgtgtgtgac ggccgcagcg
     6481 acggttgagc gggattcggc ggcggcggcg ttctcggcac tcgcggcgtc ttgaagtgtc
     6541 cggttcaacg ccgcgtcggc gtcggcgagc cgatccagcg aggacgccag gtcctgggcg
     6601 aaggtgcgat atccggccac ggcggcgccg gtcatgtccg ccgccgcatc gtgccgcagc
     6661 acctcggccg catcggcgag ctggtgcgag acaaggccgc catctgctgg tgccgaaccg
     6721 aacaacgagt ccgcccgcgt cagcgcctcg tggacgtggc gtacgaattc atcgacggac
     6781 accctctcag tattaaccac ccagctgggt caacaatgcg gtgattgccg cggcgtcgtc
     6841 ggcagtcacc ggctgccccg cctctaccgc ctcgatcagt tgagccgaca ggccgaccgc
     6901 attaccggtt tccgcggccg acaggttcgc gcggcggcgg gcggcaaaca gccgctggcc
     6961 cagcgtcgcg tcgggcgcgg cggcagcgcg aaacatcaag tcctggtagc ggtttcgcac
     7021 cgcactgagt gccatcacaa tcgcgggcag gccgcggctc tgccgggccg cacgcgcgac
     7081 gaccgcctcc aactggcgca agtcggcgag aaccaccgct gcccgttgcg cgaactcggg
     7141 gctcgacggg tcgggcagcg cgtcgatggc cgtggcgaac gtcttgatcg cgacgtcgac
     7201 cgcgccgacg atcagcgggg cctcgtcggt tccgcgctcc ggctcgccgc cctcgccggg
     7261 cacggcgccg ccgttgcgga tattcgcgat cgttcccggc ggccactgca gcacctcttc
     7321 gagcttggcc cgggtgcgct ctcgcggcca gctgcggccc ttctcgaacg cgatcagcgc
     7381 gccggcgttc atgatccggt acttcgcgag tgtgcgctgg gtgatgtcca gttcgcgacg
     7441 gcgcgcggcg acagcggcgc cggcacgggc gattccgctg tcgaggtgct cgtcgtcgtc
     7501 cagactgtcc ggatcgtcgt cggcgaagcc gaactcgacg gccggcccct cggggttgcc
     7561 gacgtggatg gtcagcccgt cggagatgtc gacggcggtg tggcgcactc cgtcgacgaa
     7621 catcccggtg cgggtgtcgg cgtcgacggc gcgccagccg gcccggtcga gttccaggcg
     7681 gacgtgcgcc ggcgagaccc tcgggtcggc caccggcacg tcggcgtctg gcgcacggcc
     7741 gatggtcgca ggggcgttct gcagcgggat ccggtacgcc gcacccgcgc acgtcacggt
     7801 cagcgtcggg gccggcgcag ggacgtccat cgagaatcac tttatccgcc agatccgcgc
     7861 tggcagacga ccgagaccgt atcgacgcta tggtttcgcg acccggatcc ggtggattcg
     7921 gcttccgcag gctcgcgctc catcgaggaa accgttgcga tgctatgtaa tgcctgctac
     7981 gttttgatcc attccgtcgg gaggggcttg cgat
//
LOCUS       DASWBF010000540        12104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_911620,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000540 DASWBF010000000
VERSION     DASWBF010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12104
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911620"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1513
                     /locus_tag="VF874_20225"
     CDS             116..1513
                     /locus_tag="VF874_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016933143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX9178298.1"
                     /translation="MTLVAGIDSSTQSCKVLVCDADTGEVLREGRAPHPDGTEIDPGA
                     WEAAAHAAIEAADGFDDVAAVSVGAQQHGMVCLDAAGDVVRPALLWNDVRSAGAALAL
                     VDELGGPDAWADAVGVVPLSAITVAKLRWLADHEPENADRTAAVCLPHDWLTWRLRGE
                     ADIGAITTDRSDASGTGYFDAASNTYRSDLVDLAVRGRRPELPAVLGAADGRKAAKGR
                     LFGAGLGDNAAAAIGLAVEPGDVVVSIGTSGVVSAVSPNPVHDGGGFVAGFADGTGQF
                     LPLVCTLNGARVLDAAAAMIGATHGELSRLALSAPPGCDGLVLVPYLEGERTPNRPNA
                     TGALHGLRVANATPANLARAAVEGLLCALADGIAHLVSHGVAMRRVLLIGGGARSRAL
                     REIAPAVFGVPVLAPPPAEYVALGAARQAAWALSAAAEPPAWKRPPTDQYTADPTPTV
                     RERYTAVRDLTEGTR"
     gene            complement(1598..2638)
                     /locus_tag="VF874_20230"
     CDS             complement(1598..2638)
                     /locus_tag="VF874_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020725826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9178299.1"
                     /translation="MTDDARAVVDAHIHQWDPFTTPRLVSGPAKVIRRAPFLAPLLMR
                     VFPRSAREFAGDPQYLLNRYLPDDYAADAAPAPVAAIVHVEAGWQGKRPVDCVDETRW
                     VASLPFGTSGMPSLDAIVVHADPAEPQVADVLDEHLRASPLVRGARCTAAYSPDPGIM
                     DFANRPNLLSEPAFLRGFAAVAERGLSFEIWLYGHQLPDAVTLAGEYPETMFVLNHYA
                     TPIGALAPCGRHTGRTAAERKDILRRWGDDISALAALPNVVAKHSGLGMPVLGLGHLG
                     REELSDAVAPLITHLGEAFGPQRTFWSSNYPIDKPNLALPDSIWILREVLGEGFDENL
                     MLRENARRVYRI"
     gene            2725..4113
                     /locus_tag="VF874_20235"
     CDS             2725..4113
                     /locus_tag="VF874_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX9178300.1"
                     /translation="MTRRDNVLLVHWHDLGRHLGTYGYADVVSPRLDQLAAEGILFTR
                     AHAAAPLCSPSRGALFTGRYPHSNGLMGLAHHGWEYRAGVRTLPQMLAEHGYYTALFG
                     MQHETSYPTRLGFDEFDVSNSYCDYVVARAQEWLTESAPLEGDQPFLLTAGFFETHRP
                     YPPERYVPADTAAVDVPDFLPDTPDVRGDFAAFHGAITVADAAVGRLLDTLSHTGLDA
                     STWVVFFTDHGAPFPRQKSTLYDGGTGIAMIVRPPTRLGVAPRVYDELFSGVDFLPTL
                     LELMGVDVPADVEGMSHAQNLLAPATDTVAIRDHVYAEKMYHDSFDPIRAIRTKQFSY
                     IENYANRPLLDLPWDIEESPSGKAVEPFVSGPRPDRELYDLLEDPAELNNLLAGDSSD
                     RAEAIADNLAVQLTEWRQKTNDVIPSEFAGTRISVRYTETYLSIHRGRTPTSRSAIAA
                     ERGIEDEVRTKQ"
     gene            4179..4982
                     /locus_tag="VF874_20240"
     CDS             4179..4982
                     /locus_tag="VF874_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HEX9178301.1"
                     /translation="MADQPSSYLVLASQRSGSTLLVESLRATGVAGEPQEFFQYLPTT
                     SQPPQPRQWFAGADDGSILRLLDPLDEGTPDLAPAEIWRDYIRTVGRTPNGVWGGKLM
                     WNQTPLLLDRASQLPHRSGDGLLAAIRDVIGEVPVLVHVYRPDVVSQAVSFWRAVQTR
                     VWRGRPDPVRDARAEYHADAIAHVITILREQEKGWRTWFDEEDIHPIEVPYPVLWRNL
                     TQIVATILEALGLDPRLAPAPVLERQADQRSDEWVDRYRADAEREGLPT"
     gene            4979..5899
                     /gene="cysD"
                     /locus_tag="VF874_20245"
     CDS             4979..5899
                     /gene="cysD"
                     /locus_tag="VF874_20245"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX9178302.1"
                     /translation="MTLVDTDARRVDELRLLEAEAVHIIREVVAELERPVLLFSAGKD
                     SIVLLRLAEKAFRPALLPFPVMHVDTGHNFREVIEFRDRRITGQGHKLIVASVQESID
                     AGRVPDPGPGASRNRQQTRTLLDALEAGGFDAAFGGARRDEERARAKERILSFRDEFG
                     QWDPRAQRPEPWSLYNGRIRKGEQVRVFPLSNWTELDVWRYIELENLELPSIYFAHER
                     EVFERDGILLAVSEYAAPINGETSAVEWVRYRTVGDLTITGAVRSEAIDITRVIAEIS
                     AATVSERGETRADDRTSAAAMEDRKREGYF"
     gene            5899..7761
                     /gene="cysC"
                     /locus_tag="VF874_20250"
     CDS             5899..7761
                     /gene="cysC"
                     /locus_tag="VF874_20250"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019746327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX9178303.1"
                     /translation="MVTATLTRQLLRIATAGSVDDGKSTLIGRLLHDTDSLPLDHLEA
                     VTDEQGVADLAALSDGLRAEREQGITIDVAYRFFSTEARSYILADTPGHERYTRNMFT
                     GASNAHVAILLVDARAGVLRQTRRHAWIAKLVGVKHFVATVNKIDLIDFDRDRFLEVE
                     QELLQMTARLGGADLTVIPIAAKHGDNVVHRSERTPWYSGPTLLEYLEGVELAAPHAE
                     ARRLRLPVQWVSRPTASQRRRYTGRLSAGTLSVGDPVVSLPSGTRSTVTAVDTLDDDR
                     TIAVAPLSVSIELADDIDVGRGDVFVSAADDAVLPVLAREIEATVCWFVDSPLRAGDR
                     LALKQATSSMRTTVQELHTRLDPETLDELDQPVDLGLNDIGTVTLRTSSVLVADAYAQ
                     NRDSGAFILIDETTNDTVGAGTILEPREVKPGTHSRTDIRWHPSALDRAYRWRKTGQP
                     GATIWFTGLPASGKSTIAVGVERALVESGRVAYLLDGDNLRHGLSDDLGFSPGDRAEN
                     IRRVGHLTRLLADAGVVALASLVSPLKSDREIARALNEAAKLPFIEVYVATPLAECER
                     RDPKGLYARARKGEVKGLTGVDAPYEPPENADLVLDTAGADLDELVARVIALLP"
     gene            7859..8704
                     /locus_tag="VF874_20255"
     CDS             7859..8704
                     /locus_tag="VF874_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178304.1"
                     /translation="MGEVFKGSEAITQGELTGWALRRWYRPIFRDVYVPKQHVLTVQD
                     RTVGAWLWSKRNAVITGVAASALHGAEWVQADIPIELRMDCSRPPRGIIARDNNLLSD
                     EVMYVSELRVTTPARTAFDLGRYLRRGQALARLDALMRATPFSVEDVQLLIKRYKGAR
                     GIRQLRERLPLVDGGAASPRETWLRLLYIDAGLPKPTTQIPVVENGRLVRMLDMGWED
                     FMVAAEYDGEQHQTSRLQYLKDMDVLPRLARLGWIVDRVVKEHREADIIRRAYNAMTS
                     RGWKP"
     gene            8786..10534
                     /locus_tag="VF874_20260"
     CDS             8786..10534
                     /locus_tag="VF874_20260"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9178305.1"
                     /translation="MALTPLRVLEDLIVDALADTGTATPAEVITRIGADPRFTPTAED
                     LAPDPDHPDRPTWHSRVYSARRHLGEDDLVVSEHGRWRLTDAARAAAPLHEPVDQPRT
                     PLQPSVIAAPLMDPAVRNRLMGRRVRDDDAIPVVIELNVAFKGGVAAAYQAFTGLLRS
                     LQMADEVPELFDDYVPTRMSMTDIQRLVREDAKEPDPIKRAIFRVWPNFPVHPQIDIS
                     ARTIKADAARRSFNARGGGITWAVVDSGIDASHPHFTGGNTLGAPDVKDLHRDFTHTL
                     RPGEDTKDAALHDGYGHGTRVASIIAGYLPADFPIDDVDVISEQITETDDPNPIPTKR
                     MVNPASLSGMAPDTHLVSLKVLADDGTSTMMAVMAALRYVRELNGDNDKVTRIHGVNV
                     SLGYEFDPQWFACGQSPLCKEVDRLVRSGVVVVVAAGNTGYARFSSWERSTSAGLTMS
                     INDPGNADRAITVGSTHRGSPHTYGVSFFSSKGPTGDGRLKPDLVAPGERITSASKVT
                     PGGPRATYSDESGTSFAAPHVSGAIAAFLSARPEFITCPDEVKKIFVESAVSLGRERT
                     FQGSGLVDVMRALQSV"
     gene            10553..11953
                     /locus_tag="VF874_20265"
     CDS             10553..11953
                     /locus_tag="VF874_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-threonine protein kinase"
                     /protein_id="HEX9178306.1"
                     /translation="MADPPLQIQFDEDGDINAEQERQLHDALEQLGATDLLVFSHGWN
                     NTPAVATQLYEAFFTRVKSLMALHGKANRTVAYLGVYWPSIRWSDEPIPDFQPGDVVD
                     LGAGSGGAAGGVGPPPTFDPPPPPDTQLAAQIRESFPADLRGDVGELIELITARPTEQ
                     DQLRRARDLVAQIASAAPADGDGEQADRPPFAAEDDPNAQLFADFTAALEELNVETTG
                     AGGAAGIGDAVGRLWHGAQEVLRGLTYWQMKNRAGVVGERGLGPLVGRLRERHPNLNI
                     NLIGHSFGARVVSYALKGAPQDPPSVRSVTLLQGAFSHFTYTAELPFDTSRSGTLNGQ
                     QDKVSGPVAACYSRFDSAVGVMYPLASLLKGDDASAADEPLYRWGGIGHDGHQQGLPE
                     LTLQQPGSHYDFAGKKLVNIDAQQVVREGRPPSGAHSDIIHDELAWVVLDAGGLVDAA
                     HVTSETDAAHVRSETD"
BASE COUNT         2057 a         4080 c         4038 g         1929 t
ORIGIN      
        1 gctcggtgtt tggtgatctc gtcgacgagc cacggttcgt ggagccctat ctgtggacgc
       61 tggagtcgtt gcaccgcaac gggtctcgga caacgctgga agcgctgctg aagtcatgac
      121 gctcgtcgcc ggaatcgact cctcgaccca gtcgtgcaag gtgctggtgt gcgacgccga
      181 cactggcgaa gtgctccgcg agggacgcgc tccacatccc gacgggaccg aaatcgaccc
      241 gggcgcttgg gaagccgcgg cacacgctgc gatcgaggcg gccgacggat tcgacgacgt
      301 cgctgcggtg tccgtcggcg cacagcaaca cggcatggtg tgcctggacg ccgcgggcga
      361 tgtcgtcagg cccgcgctgc tgtggaacga cgtcaggtct gccggcgcag cactcgcctt
      421 ggtcgacgag ctcggcggtc ccgacgcgtg ggcggacgcg gtcggtgtcg tgccgctgtc
      481 ggcgatcacc gtcgcaaaac tgcggtggct cgccgaccac gagccggaaa acgccgaccg
      541 caccgccgcg gtgtgcctac cgcatgactg gctgacctgg cgcttgcgcg gcgaagccga
      601 catcggcgcg attaccaccg accgcagcga tgcgagcggc acgggctact tcgacgcagc
      661 gagcaatacc tatcgcagcg acctcgtcga tctcgcggtg cgtgggcgcc gtcccgagct
      721 gccagcggtg ctcggcgcgg ccgacggaag gaaggccgcc aaaggtcggc tcttcggcgc
      781 cggcctgggc gacaacgccg ccgccgcaat aggattggcc gtcgagccgg gcgacgtcgt
      841 cgtctctatc ggaacatcgg gcgtggtgtc ggcggtatcg ccgaatcccg tgcacgacgg
      901 gggcggattc gtcgccgggt tcgccgacgg caccgggcag ttcctgccgt tggtgtgcac
      961 gttgaacggc gcccgtgtgc tcgatgccgc cgccgcgatg atcggagcga ctcacggaga
     1021 gctctctcgg ctggcgttgt ccgcgccgcc cggatgcgac ggccttgtgc tggtgcccta
     1081 cctcgaaggc gagcgaaccc ccaaccggcc caacgcaacc ggcgcgctgc acgggttgcg
     1141 ggtggcgaac gcgacgcctg ccaatctggc gcgggccgcg gtggagggac tgttgtgcgc
     1201 gctcgccgac ggcatcgcac acctcgtctc gcatggtgtc gcgatgcgcc gcgtgctgct
     1261 gatcggcgga ggggcccggt cgcgcgcgct gcgcgagatc gcgcccgcgg tgttcggtgt
     1321 cccggtgctc gcaccaccgc ccgccgagta cgtggcgctc ggcgcggcgc gccaggccgc
     1381 gtgggccctg agcgccgcag cggaacctcc cgcgtggaag cggccgccga ccgatcagta
     1441 caccgccgac ccgacgccca ccgtccgcga acggtacacg gccgtccgcg atctgacaga
     1501 gggaacccgg tgagccatag cgcgctcagc ctttcgccga aagtgaatcc agggctgtag
     1561 aaccgcgaca atcacgaccc ggaattctat ttcggcgtta gatccggtag acgcgtcgcg
     1621 cgttctcccg cagcatcagg ttctcgtcga aaccttcgcc cagcacctca cgtagaatcc
     1681 aaatactgtc cggtagagca agattcggct tgtcgatcgg atagttcgac gaccagaacg
     1741 ttcgctgcgg gccgaaagcc tcaccgaggt gagtgatcaa cggcgccacc gcatcggaca
     1801 gctcttcgcg tcccagatgt ccaagaccca gcaccggcat gcccaaaccc gagtgtttgg
     1861 caaccacgtt cggcaacgca gccagcgcgg agatgtcgtc accccaccgc cgcaggatgt
     1921 ccttgcgctc agcggcggtg cgtccggtgt gtcggccgca gggggcgagc gctccgatcg
     1981 gtgtcgcata gtggttcaac acgaacatcg tctcggggta ctcgcctgcc agcgtgacgg
     2041 catccggtaa ctggtgaccg tagagccaga tctcgaaact gagaccgcgc tcggccaccg
     2101 cggcgaaccc ccgcaggaac gccggctcgg agagcaggtt cggcctgttc gcgaaatcca
     2161 tgatcccggg gtcggggctg tacgcagccg tgcaccgcgc gccccgcacc agaggactcg
     2221 cccgcagatg ctcgtcgagc acatcggcca cctgcggctc ggccggatcg gcgtgcacga
     2281 cgatcgcgtc caggctcggc atcccggacg tgccgaacgg caaggaggcg acccaccggg
     2341 tttcgtccac acaatcgacc ggtcgtttgc cttgccagcc cgcctcgaca tgcacgatgg
     2401 ccgccacggg cgccggcgcc gcgtcggcgg cgtagtcgtc cggtagatac ctgttgagca
     2461 ggtactgagg gtcgccggcg aattcgcgcg cggaccgcgg gaacacccgc atcagcagcg
     2521 gcgccaggaa cggcgcccgt cggatgactt tcgccggccc cgacacgagc cggggcgtcg
     2581 tgaacggatc ccactggtgg atatgcgcgt ccaccaccgc gcgtgcgtcg tctgtcacga
     2641 tgacagtttg gtgctaggcc gctcgccgat ccggatgatc gaaagaattg gcatcggggc
     2701 ggccgggggt ggaaggtggc gaacgtgacg cgacgcgaca acgtcttgct cgtgcattgg
     2761 cacgacctcg gccggcatct cggcacctac ggctacgccg acgtggtgag tccccgcttg
     2821 gaccagctcg ccgccgaagg catcctgttc acccgcgcgc acgccgccgc gccgctgtgc
     2881 tctccgtcgc gcggagcgct gttcaccggg cgctaccctc acagcaacgg cctgatgggt
     2941 ctggcacatc acggctggga gtaccgagcc ggcgtccgca cgctgccaca gatgctggcc
     3001 gaacatggtt attacacagc gcttttcggg atgcagcatg agacctcgta cccgacccgg
     3061 ctggggttcg acgagttcga cgtgtccaac tcgtattgcg actacgtggt ggctcgggcg
     3121 caggagtggc tcaccgagag cgctccactg gagggcgacc agccgttcct gctgaccgcg
     3181 ggctttttcg aaacccatcg gccgtacccg cccgagcggt acgtacccgc cgacactgcc
     3241 gccgtcgacg tacctgactt tctgcccgac actccggacg tgcggggtga cttcgccgcg
     3301 ttccacggcg cgatcaccgt ggcggacgcc gcggtcgggc ggctgctcga cacgttgtcc
     3361 cacaccggtc tggacgccag cacctgggtg gtgttcttca ccgaccatgg agcgccgttc
     3421 ccgcgccaga agtcgacgct gtacgacggc ggcaccggga tcgcgatgat cgtccggccg
     3481 cccactcggt taggcgtcgc gccgcgggta tacgacgagc tgttcagcgg cgtcgatttc
     3541 ctgccgaccc tgctcgagct gatgggtgtc gacgtaccgg cggacgtcga ggggatgtct
     3601 cacgcgcaga acctgctggc cccggccacc gacaccgttg cgattcgcga ccacgtgtac
     3661 gcggaaaaga tgtaccacga ctcgttcgat ccgattcgtg cgattcggac caagcagttc
     3721 agctatatcg agaattacgc gaaccgccca ttgctggatt taccgtggga catcgaagaa
     3781 agcccgtccg gaaaggcagt cgaaccgttc gtgtcgggcc cgcgccccga tcgggaactc
     3841 tacgacctgt tggaagatcc ggccgagctg aacaaccttc tggccggcga cagcagcgat
     3901 cgcgcagagg ccatcgccga caacctcgcg gtgcagctga ccgaatggcg ccagaagacc
     3961 aatgacgtga ttccgtcgga gttcgcggga acccggattt ccgtccgcta caccgagaca
     4021 tatctgagca tccatcgcgg gcgtaccccg acgtcgagat cggcgattgc cgccgagcgt
     4081 ggcatcgaag atgaggtccg cacaaagcaa tagttatgct catcgtgaaa agaaaccgct
     4141 ggtcggcagg gaattcacta tcccgttagg cttccgtcgt ggccgaccaa ccgtcgtcgt
     4201 atctggtact cgcctcgcag cgcagcggca gcacgctgct agtcgagtcg ctgcgcgcga
     4261 ccggtgtggc cggcgaaccg caggagttct tccagtacct gccgaccacc agtcagccgc
     4321 cgcagccgcg ccagtggttc gccggggctg acgacggttc gatcctgcgg ctgctggatc
     4381 cgctcgacga gggcaccccc gatctggcac cggccgaaat ctggcgtgac tacatccgca
     4441 cggtcggcag gacaccgaac ggcgtgtggg gcggcaagct gatgtggaac cagacgccgc
     4501 tactgctgga ccgcgccagc cagctgcccc accgctcggg cgacggtctg ctcgccgcga
     4561 tccgcgacgt gatcggcgag gtcccggtgc tcgtgcacgt ctaccgcccc gacgtggtct
     4621 cgcaggcggt gtcgttttgg cgggccgtgc aaacccgggt ctggcggggc cgccccgacc
     4681 cggtgcggga cgcacgcgcg gaataccacg ccgacgcgat cgcgcatgtc atcacgatcc
     4741 tgcgcgagca ggaaaagggt tggcggacat ggttcgacga ggaggacatc catccaatcg
     4801 aggtccccta ccccgtgctg tggcgcaacc tgacacagat cgtggcgacg atcttggagg
     4861 cactgggcct cgatccgcgg ctcgcacccg caccagtact ggagcgccag gccgaccaac
     4921 gttccgacga gtgggtggac cgctaccgcg cggacgccga aagagagggg cttcccacat
     4981 gacattggtt gataccgatg cccgccgggt cgacgaactg cgactgctgg aagccgaggc
     5041 ggtgcacatc atccgggaag tcgtcgccga gctcgagcgg ccggtgctgc tgttctcggc
     5101 cggtaaggac tcgatcgtgc tacttcggtt ggcggagaag gcttttcggc ccgcgctgct
     5161 gccgtttccg gttatgcacg tcgacaccgg ccacaacttc cgtgaggtca tcgagtttcg
     5221 cgaccgccgg atcaccggtc aggggcacaa gctgatcgtc gcgtcggtgc aggagtcgat
     5281 cgacgcgggc cgagtgcccg accccggacc cggcgcgtcg cgcaaccggc agcagacccg
     5341 cacgctgctg gacgcgttgg aggccggtgg cttcgacgcg gcgttcggcg gcgcccgtcg
     5401 cgacgaggag cgggcccgcg ccaaggaacg catcctgagc ttccgcgacg agttcggcca
     5461 gtgggacccg cgcgcacagc ggcccgagcc gtggtcgctg tacaacggac ggattcgcaa
     5521 gggcgagcag gtgcgggtgt tcccgctgtc gaactggacc gagctggacg tctggcgcta
     5581 catcgagctg gaaaaccttg agctgccgtc gatttacttc gcccacgagc gcgaggtgtt
     5641 cgagcgcgac ggcatcctgc tcgcggtgtc cgagtacgcc gcacctatca acggagagac
     5701 gtccgcggtg gagtgggtgc gctaccgcac cgtcggcgac ctgacgatca ccggcgcggt
     5761 gcgctcggag gccattgaca tcacccgcgt gatcgccgag atctccgcgg cgacggtgtc
     5821 cgaacgcggc gagacccgcg ccgacgaccg gacgtcggcc gccgcgatgg aagaccgcaa
     5881 acgcgagggg tatttctgat ggtgacggca accttgacgc ggcagctgct ccggatcgcg
     5941 accgcgggct ctgtggacga cggcaagagc acgctgatcg gccggctgtt gcacgacacc
     6001 gacagcctgc cgctcgacca cctcgaggcg gtcaccgacg agcagggggt cgccgacctg
     6061 gccgcgctgt ccgacgggct gcgggccgaa cgcgagcagg gcatcacgat cgacgtcgcg
     6121 taccggttct tctccaccga agcccgcagc tacatcctgg ccgacacgcc gggccacgag
     6181 cgctacaccc gcaacatgtt caccggcgcg tccaatgcac acgtcgcgat tctgctcgtc
     6241 gacgcccgag ccggtgtcct gcggcagacc cgtcgtcacg cctggatcgc caagctcgtc
     6301 ggcgtcaaac acttcgtcgc gacggtgaac aagatcgacc tgatcgactt cgaccgggac
     6361 cggttcctcg aggtcgagca ggagctgctg cagatgactg cccgcctcgg tggtgccgac
     6421 ctcacggtga tcccgatcgc ggcgaagcac ggcgacaacg tggttcaccg gtctgagcgg
     6481 actccgtggt acagcgggcc gacactgctc gagtacctgg aaggcgtcga gctggccgcg
     6541 ccgcatgccg aggctcggcg gttgaggttg ccggtgcagt gggtgtcacg accgacggct
     6601 tcgcagcggc ggcgctacac cgggcggctg tcggccggca cactgtcggt gggtgacccg
     6661 gtggtgtcgc tgccgtccgg cacccggtcg acggtgacgg cggtggacac gctcgacgac
     6721 gaccgtacga ttgccgttgc gccactgtcg gtttcgatcg aactcgccga cgacatcgac
     6781 gtcggacgcg gcgacgtgtt cgttagcgcc gccgacgacg cggtgctgcc ggtgctggcg
     6841 cgcgagatcg aggcgacggt gtgctggttc gtcgactcgc cgctgcgggc cggcgaccgg
     6901 ctcgcactga agcaggccac ctcgtcaatg cggaccaccg tgcaagagtt gcacacccgg
     6961 ctggacccgg aaaccctcga cgagttggac cagccagtcg acttggggct caacgacatt
     7021 ggcaccgtga cgctgcgcac cagctcggtg ctcgtcgcgg atgcttacgc ccagaaccgc
     7081 gacagcggtg cgttcatcct gatcgacgag accaccaacg acaccgtcgg tgcgggcacc
     7141 atcctcgaac cccgcgaggt caagccgggc acgcactccc gcaccgacat ccggtggcat
     7201 ccgtcggcac tggaccgcgc ataccgctgg cgaaagaccg gccaacccgg agcgacgatc
     7261 tggttcaccg ggctgccggc ctccggtaag tccacgatcg cggtcggagt ggagcgcgca
     7321 ctggtggagt ccggccgggt ggcctacctg ctcgacgggg acaacctgcg gcacggcctg
     7381 tccgacgacc tcgggttctc gcccggcgat agagccgaaa acatccgccg cgttgggcat
     7441 ttgacgcgtc tcttggccga tgccggtgtg gtggcgctgg cctcattggt gtcgccgctg
     7501 aagtccgatc gcgagattgc ccgcgcgttg aacgaggccg caaagctgcc gttcatcgag
     7561 gtgtacgtcg cgactccgtt ggcggagtgc gaaaggcgag acccgaaggg cctgtacgcc
     7621 cgcgcccgca agggcgaagt caaggggctg acgggcgtcg acgcgcccta cgagccgccg
     7681 gagaacgccg acctcgtact ggacaccgcc ggcgcggacc tcgacgagct cgtcgcgcgc
     7741 gtcatcgccc tgctccccta gttcgcgccg aaacggtatt ccacgtggtc cgttctcgca
     7801 cttttccgca gcagttaccg cctcggcgca tgtcggtgga agctggcact ctccggacat
     7861 gggggaagtc ttcaaaggta gcgaggcgat cacgcaagga gagctgaccg gatgggcatt
     7921 gcggcggtgg taccggccca tcttccgaga cgtttatgtt ccgaagcagc acgtcctcac
     7981 cgttcaagat aggaccgttg gcgcgtggct gtggtcaaag cgcaatgccg tcatcaccgg
     8041 cgtggccgcg tcggccctgc acggcgcgga gtgggtgcaa gcggacattc cgatcgaatt
     8101 gcggatggat tgcagtcgtc cgccccgcgg aatcatcgca cgggacaaca acttgctcag
     8161 cgacgaggtg atgtacgtca gcgaacttcg cgtgactacg cctgcgcgta ccgcgttcga
     8221 tctcggccgg tatctgcgac gagggcaggc gctggcgcgg ctcgacgcgc tgatgcgggc
     8281 gacgccattc tcggttgaag acgtgcagtt gctgatcaag cgctacaagg gcgcgcgcgg
     8341 cattcgccaa ttgcgggaac ggctgcctct tgtcgacggc ggcgccgcgt ctcccaggga
     8401 gacctggctg cgactgctct acatcgatgc cggattaccg aagcccacca cgcagatacc
     8461 cgtcgtcgag aacgggcggc tagttcgcat gttggacatg ggctgggaag acttcatggt
     8521 cgccgccgaa tacgacggcg agcagcacca gaccagtcgt ctacagtacc tcaaggacat
     8581 ggacgttctg cccaggcttg ctcgcctggg ctggatcgtg gaccgcgtcg tcaaagagca
     8641 ccgcgaggcc gacatcattc ggcgcgccta caacgcgatg acctcgcgag gctggaagcc
     8701 ctgagttcgc ccagacggta accaacgtgg tctccactcc aactattccg cgtggaatac
     8761 cgtttcggcg agatagcgtg gacgcatggc gctgacgccg ctgcgcgttc tcgaggacct
     8821 gatcgtcgac gcgcttgccg acaccggtac cgctacgccg gcggaggtga tcacacggat
     8881 cggcgcggac cctcggttca ctcccaccgc ggaggatctc gcgcccgacc ctgaccaccc
     8941 cgaccgcccg acgtggcact cccgggtcta ttcggcgcgc cgccaccttg gcgaggacga
     9001 tctggtggtc tccgagcacg gccggtggcg gctcaccgat gccgcccgcg ctgcagcacc
     9061 gctgcacgaa cccgtcgacc agccgcgaac tccattgcag cccagcgtga tcgccgctcc
     9121 gctcatggac cctgcggtac ggaaccggct gatgggccgg cgggtccgcg acgacgacgc
     9181 gatcccggtt gtcatcgaac tgaacgtggc gttcaagggc ggcgtcgccg cggcatatca
     9241 agcattcacc gggctgctcc gctcgctgca aatggccgac gaggtgccgg agttgttcga
     9301 cgactacgtg ccgacacgga tgtcgatgac cgacattcag cggctggtcc gcgaggacgc
     9361 caaagaaccc gacccgatca agcgggcgat cttccgggtc tggccgaact ttccggttca
     9421 tccgcagatc gacatctcgg cgcgaaccat caaggccgac gcagcgcgcc gctcgttcaa
     9481 cgcccgcggc ggcggaatca cttgggcggt cgtcgattcc ggcatagacg ccagccatcc
     9541 gcacttcacc ggcggtaata cgctcggcgc gccggatgtc aaggatctgc accgcgactt
     9601 cacccacacg ctgcggcccg gcgaagatac caaagacgca gcgctgcacg acggttacgg
     9661 ccacggcacg cgcgtcgcga gcatcattgc cggctacctg cccgccgatt tcccgatcga
     9721 cgacgtcgac gtgatcagcg agcagatcac cgaaaccgac gacccgaatc cgattccgac
     9781 caagcgcatg gtcaatcccg catcactgtc gggcatggcg ccggacaccc acttggtcag
     9841 cctcaaggtc ctcgccgacg acgggacgtc gacgatgatg gcggtgatgg cggcgctgcg
     9901 ctatgtgcga gaactcaacg gcgacaacga caaagtcacc cgaatccacg gggtcaacgt
     9961 gtcgttggga tacgagttcg acccgcagtg gttcgcctgc ggtcagagtc cgctgtgcaa
    10021 ggaggtggat cggctcgttc gctccggcgt ggtggtcgtc gtggccgcgg gcaacaccgg
    10081 ctacgcgagg ttcagctcgt gggagcggtc caccagcgcc ggattgacca tgagcatcaa
    10141 cgaccccggc aacgccgacc gtgcgatcac cgtcgggtcg acgcaccgcg gatcaccgca
    10201 cacctatggg gtgtcgttct tttcgtcgaa gggcccgacc ggcgacggcc ggctcaagcc
    10261 cgacctggtc gcgcccggtg aacgcatcac ctcggcgagc aaggtgactc ccggcggccc
    10321 gcgcgccacc tattccgacg agtccggaac cagtttcgcc gcaccgcatg tgtcgggcgc
    10381 gatcgccgcg ttcctgtccg cgcggccgga gttcatcacc tgccccgacg aagtgaagaa
    10441 gatcttcgtc gagtcggcgg tgtcgctggg ccgcgagcgc acgttccagg gcagcgggct
    10501 cgtcgacgtg atgcgcgcac ttcaatctgt ctgaccgaca aggagattca tcgtggccga
    10561 tcctccgctg cagatccagt tcgacgagga cggcgatatc aacgctgagc aggagcgcca
    10621 actgcacgac gcgctggagc agctcggcgc gaccgatctg ttggttttca gccacggctg
    10681 gaacaacacg ccggcggtcg cgacgcagct ctacgaggcg ttcttcactc gcgtgaagtc
    10741 gctgatggcc cttcacggca aagcgaaccg cacggtcgcg tatctcggcg tgtactggcc
    10801 gtcgatccgg tggtccgacg aaccgattcc cgacttccag ccgggcgacg tcgtggactt
    10861 gggtgccggg tccggtggcg cggcaggcgg ggtggggccg ccgccgacgt tcgatccccc
    10921 gcctcctccg gacacgcaac tggccgcgca gatccgcgag tcgtttcctg ccgacctgcg
    10981 cggcgacgtc ggcgagctca tcgagctgat cacagctcga cccacagagc aggaccagct
    11041 gcggcgggcc cgggaccttg tcgcacagat cgccagcgct gctccggcgg acggcgacgg
    11101 tgagcaggcc gaccgcccgc cgtttgcggc cgaggacgac ccgaatgccc agctgttcgc
    11161 cgacttcacc gccgcgctcg aggaactcaa cgtcgaaacc accggcgccg gcggagcggc
    11221 gggcatcggc gacgctgtgg gtcggttgtg gcacggcgct caggaggtgc tgcggggtct
    11281 cacgtactgg cagatgaaga accgcgccgg tgtcgtcggc gagcggggcc tgggtccgct
    11341 tgtcgggcgg ctgcgcgaac gacatccgaa tctgaacatc aatctgatcg gccacagctt
    11401 cggtgcccgg gtggtgtcct acgcattgaa aggtgctccg caggaccctc cgtcagttcg
    11461 gtcggtcacc ctgttgcagg gcgccttctc ccatttcacc tacacggccg agttgccgtt
    11521 cgacaccagc cgatccggca cgttgaatgg ccagcaggac aaggtatccg gcccggtggc
    11581 cgcctgctat tcccgcttcg acagcgcggt aggcgtgatg tacccgctgg cgtcactact
    11641 gaagggcgat gacgcctcgg ccgccgacga gccgctgtat cgctggggcg gcatcggaca
    11701 cgacgggcat cagcagggac tgccggagct gacgctgcag cagcccggca gccactacga
    11761 cttcgccggc aagaagctgg tgaacatcga cgcccagcag gtggtgcgcg aagggcggcc
    11821 accgtcgggc gcgcattcgg acatcatcca cgacgaactg gcctgggtgg tgctcgacgc
    11881 tggcgggctg gtagacgccg cgcacgtgac gagcgagaca gacgccgcgc acgtgaggag
    11941 cgagacagac tgagcgctct cgccgccaag gatttgttga gaatctggca aggcccggcg
    12001 ctgattctgg gatcagccgc aacaccgcgg acaccacaac ccacatcagc accgaaggag
    12061 ttccaatgtt cacccgccgt ttcaacaagt ccatcgccag cacc
//
LOCUS       DASWBF010000541         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_718537,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000541 DASWBF010000000
VERSION     DASWBF010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718537"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..1464
                     /locus_tag="VF874_20270"
                     /pseudo
     CDS             498..1464
                     /locus_tag="VF874_20270"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1461..2387
                     /locus_tag="VF874_20275"
     CDS             1461..2387
                     /locus_tag="VF874_20275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9178307.1"
                     /translation="MTGVRDALADYLQLRRRLGFEMPQDGRLLEGFVTFLEAAGAERI
                     STDLALAWARSPGQAHPSYWGQRLTVVRGFARHLATLDPASEIPAQDLLPAHRPRIAP
                     YIYTDDEIAALIAAAGRLTPPLRALRHQTLIGLLAVTAMRPAEALALDRDDVDLRHRA
                     VLVRIGKQHKQREVPLHDSTVTALGEYVRQRDARFPQPTTPAFFISARGGRVARHELN
                     QTFTQLIRQAGLDGRGTRARPRPYDLRHAFAVRTVLDWYAAGDDVDRKMPLLSTFLGH
                     ADPASTYWYLQAVPELLAVISSRLERLPGVSS"
     gene            2384..>3352
                     /locus_tag="VF874_20280"
     CDS             2384..>3352
                     /locus_tag="VF874_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9178308.1"
                     /translation="MSALAPTVQAFFTERLITQRNASPHTIAAYRDTFRLLLRFAQDQ
                     TGKQPFQLDLDDLDAPLIGAFLTHLEQVRGNNPRTRNARLGAIHSFYRFAALEHPEHA
                     HTIARVMAIPTKRHQRNTVSYLDTGEITALLAAPDRRTWLGRRDYALMVLMIQTGIRV
                     SELTGLRIGDVHLGVGAHIRVTGKGRKMRSPTLTHETVAVIRAWLTELRGGQPDQPLF
                     PTRQGRPLSRYTVGAIITRHVTTAAVTCTTLPAKRITPHTLRHTNAMLLRANNVDIAT
                     IALWLGHETTQPTHIYEHADPALKERAIARTAPLGAKPGRYHAPDEL"
BASE COUNT          517 a         1216 c         1090 g          529 t
ORIGIN      
        1 gccgacaccc catgcccacg tccacaaaac cgaccacttg acttcttcct cataggctgc
       61 tcagaaccgg ttatgtggag ttccacataa ccggttgtat gtcccacgca cggttatgtt
      121 gaatgctgac ctgcctggag cgatttccct tgcagcgcag ggtttgacag atcagacgtt
      181 ccacataatc cgcctgtcca tcgatgctcc ttggatccag ttatgggatc tgaaaggagt
      241 gggcgatggg caggaaggtg tggtacccgg cggtgtcggg gccattgtcg gcgtatgcgg
      301 cggggtatgt ggcgtggatg cgggcgcgaa gctattcgcc ctcggcgatt tcggacaggt
      361 tgtggcagtt cgatcagctg agccgctggc tcgatcgcga ggggcttccc gtcgtcgagt
      421 tgaccctcgc gcaatgcctg cggttcgccg cgtcgcggcg tgctgccggc gttgtgacgt
      481 tggtggcccc gcagagcatg acgctgccat acgagtattt gcgggcgctc gggttgacgc
      541 cggcaccgcc gcctgtgccg cacggtccgg tggaggatct gctcgctgac tacggccgct
      601 acctgctgat cgagcgcggc ctgacaccgc acaccgtgcg ggacgcctac gtgccggcgg
      661 cgcggttgtt cctcgccggc cggggcggcc ctggcgggct gcgcttggac cggctcgctc
      721 ccgccgatgt gagcatgttc ctggcgcggg agtgcccgca gcgcaccctg tcgggagcgc
      781 gggatctggt gtccgcattg cgctcgtttc tgcgttatct gcacctgtcc ggcctgatcg
      841 aggtgccgct ggtgtgggcg ctgcccccga tcgcggacct gcgggatcgc acgctgccgc
      901 ggggcttgga gccggtggcg gtcacgcggc tgctggccag ttgcgaccgg cgcagactgg
      961 tcgggcggcg tgacttcgcg atcctgctgc tgttgtcgcg tctggggctg cgggcgggcg
     1021 aggtcgccgg gttacgcctc gacgacgtcg actggcgtcg cgggctgctg ctcatccgcg
     1081 gcaagggcgg ccgctacgac gagctgccgt tgcccgacga tgtcggccag gcgctggtgt
     1141 cgtacctgcg gcgtcggccg cgttgccagt cgcggggcgg tgtttgtgcg ggtgaccgca
     1201 ccgcgccggg agctgaaccg gtccacgatc ggctgggtcg tgcgtgccgc atgtgaccgg
     1261 gccggtctgc ccagggtcgg cgcgcatcgg ctgcggcaca ccgccgcgac ccaaatgctg
     1321 cgtcgcggcg catcgctcgc cgagatcggc gaagtgttgc gccatcgcga gcagaagacc
     1381 accgcgatct acgcgaaggt cgaccgcacg gcgctgcggc cgctcgcgcg gccgtggccg
     1441 acaacgcggg caggtacggc atgaccggcg ttcgggacgc cctggctgac tatctgcagc
     1501 tacggcgccg acttgggttc gagatgccgc aggacggccg cctgctggaa gggttcgtga
     1561 ccttcctcga ggcggccggc gcggagcgga tcagcaccga tctggcgctg gcgtgggcac
     1621 ggtcaccggg gcaggcgcac ccgtcttatt ggggtcagcg gctgaccgtg gtgcgcgggt
     1681 tcgcgcgcca cctggcgacg ctcgacccgg ccagcgagat cccggcgcaa gacctgctgc
     1741 cggcacatcg gccacgcatc gccccctaca tctacaccga tgacgagatc gcggcattga
     1801 tcgcggctgc cggacggctc acgccgccgc tacgcgcgct acggcatcag acgctgatcg
     1861 ggctgctcgc ggtcaccgcg atgcgcccgg cagaggcgct cgcgctcgac cgcgacgacg
     1921 tcgacctgcg acaccgcgcg gtcctcgtcc gcatcggcaa gcagcacaag cagcgcgagg
     1981 tcccgctgca cgacagcacc gtcaccgcgc tgggcgaata cgtccgccag cgtgacgccc
     2041 gcttcccgca accgacaacg ccggcgttct tcatctccgc ccgcggcggg cgggttgccc
     2101 gccacgaact caatcagacc ttcacccagc tgatccgtca ggccggcttg gacggccgcg
     2161 gcacgcgcgc caggccgcgg ccctatgacc tgcgccatgc cttcgccgtg cgcaccgtgc
     2221 tcgactggta cgccgcgggc gacgacgtcg accgcaagat gccgctgctg tccaccttcc
     2281 tcgggcacgc cgacccggcg agcacgtact ggtatctgca ggcggtcccg gagttgctcg
     2341 cggtcatcag cagcaggctg gaacgattgc ccggggtgtc gtcgtgagcg cgctcgcgcc
     2401 gacggtgcag gcgttcttca ccgaacgtct gatcacccag cgcaacgcca gcccgcacac
     2461 catcgccgcc taccgcgaca ccttccggct gctactccgt ttcgcccaag accagaccgg
     2521 caaacagccg tttcagttgg acctcgacga tctggacgcg ccgctgatcg gcgcgtttct
     2581 cacccacctg gaacaggtcc gcggcaacaa cccccgcacc cgcaacgcca ggctcggggc
     2641 gatccactcg ttctaccggt tcgcggcgct cgaacacccc gaacacgcgc acaccatcgc
     2701 gcgggtcatg gcgatcccga ccaaacgtca ccagcgcaac accgtcagct acctcgacac
     2761 cggggagatc acagcactgc tcgccgcgcc cgatcgccgc acgtggctcg gccgccgcga
     2821 ctacgccctg atggtgctga tgatccaaac cgggatccgc gtctcggaac tcaccggtct
     2881 gcgcatcggc gatgtccacc tcggcgtcgg cgcccacatc cgcgtcaccg gcaagggacg
     2941 aaagatgcgc tcacccacgc tgacccacga aaccgtcgcg gtcatacgcg cctggctcac
     3001 cgaactccgc ggcggccaac ccgaccagcc gctgttcccc acccggcaag gccggccgct
     3061 cagccgctac accgtcggcg cgatcatcac ccgacacgtc accaccgctg ccgtcacctg
     3121 cacgacgctg ccagcgaaac gcatcacacc gcacaccctg cgccatacca acgccatgct
     3181 cctgcgcgcc aacaacgtcg acatcgcgac gatcgcgctg tggctcggcc acgagacgac
     3241 ccaaccaacg cacatctacg agcacgccga ccccgccctc aaggaacgag caatcgcacg
     3301 aaccgcccca ctcggagcca agccgggcag ataccacgca cccgacgaac tc
//
LOCUS       DASWBF010000542        12409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1243854,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000542 DASWBF010000000
VERSION     DASWBF010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243854"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VF874_20285"
     CDS             <1..190
                     /locus_tag="VF874_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HEX9178309.1"
                     /translation="KRQDASYTLTEEGDKTRVKFDLTVDPAIPIPGFVMKRAIKGAMN
                     DATDGLRKRVLSLKKGGR"
     gene            200..1279
                     /locus_tag="VF874_20290"
     CDS             200..1279
                     /locus_tag="VF874_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9178310.1"
                     /translation="MTNSGPLAGVRVIELGGIGPGPHAAMVLADLGADVVRVRRPGAT
                     AMPREDRDLMHRGKRVVDLDVKGEPQKLLDLAAKADVLLDPFRPGTCERLGIGPEDCA
                     AVNPRLIFARMTGWGQDGPMALTAGHDINYLSQTGALGSMGYRDRPPLPPMNLVADFG
                     GGSMLLLLGIVAALYEREKSGKGQVVDAAMVDGVSILAQMQWTMKAVGTLSDERESSL
                     LDGGAPFYRTYETSDGKYMAVGAIEPQFYAQLLDGLGLSADDLPNQLDPDGFPQMRKL
                     FTERFASKTRDGWTEIFAGTDACVTPVLTWSEAAGNEHLRARSTIVHRNGFDQAAPAP
                     RFSRTPATEFGPPPSDTTPVEDIGW"
     gene            1343..1807
                     /locus_tag="VF874_20295"
     CDS             1343..1807
                     /locus_tag="VF874_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9178311.1"
                     /translation="MAVRASREVLIDAPPEAILDALADIESAPSWSSMHKRVEIVDKY
                     DDGRPHHVKVHYKLMGITDKELLEYHWGRDWVVWDAEETFQQHAQHVEYTLRPEAGKT
                     RVRFDITMEMRAPIPKFLMKRAKKYVLDIATEGLRQQVSGGKSSVPANPEVT"
     gene            complement(1812..2303)
                     /locus_tag="VF874_20300"
     CDS             complement(1812..2303)
                     /locus_tag="VF874_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HEX9178312.1"
                     /translation="MLSLAIHLVIGIAVIVAIVASNPQIFRQPATPKVSVLEVVFYVT
                     GIAATVLGYHFSYRYVTDYSQGTHNAIWGDHGSWKEFIQLGYTNPAASNVSQDYTIMS
                     VILLPLFLLVDGRRRRVRRAWLYLLLILTTSSAFPWAFYLATVERQRRVAAASPEPAA
                     LQP"
     gene            2371..2976
                     /locus_tag="VF874_20305"
     CDS             2371..2976
                     /locus_tag="VF874_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9178313.1"
                     /translation="MVRPAQTVRSERTREALRRAALLRFLDQGVEDTSAEQIAADAGV
                     SLRTFYRHFASKHDLLFADYDAGLHWFRSALASRPSGEPLIESVQAAIFAFPYDVGVV
                     TQIASLRAAELEPDRIIRHIRHVEADFAEAIAEHLRRRRAKGDNLDGALQIAVTARCI
                     AAAIFGAMELWMVADERSLADLASMCQTALGWLDGALFRQY"
     gene            3008..4567
                     /locus_tag="VF874_20310"
     CDS             3008..4567
                     /locus_tag="VF874_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9178314.1"
                     /translation="MADYDAIVVGAGHNGLTAAVLLQRAGLRTLCLESKLYAGGMAST
                     VELFDGYQFEIAGSLQLSTSAVVSRELGLDALPTVDLDVMSVSLRGVGDDPVVYYTDP
                     LKLLTHLNEVHGAEAVNGMAGLMAWSQAPARALGRFDACQLPKTMDEMYACATNEFER
                     AAITDMLFGSVSDVLDRYLPDREKHGALRGMLAFLAVNTTYRGPETPGTAAAFAFGLA
                     VPNEDAVLTKKLRGGIGALTQHLLGLFTGDGGELRLRTPVAEILVHDGWVAGVRTEDG
                     STISAPLVVSSVAPDFTVLRLVDGGAVSSDVRERFSRVDHRGSYLQMHFALDGIPEFA
                     APYELLNDPMMQANIGLFATPEELQQQWEDCRRGIVPADPAVALQIPSVHDPALAPDG
                     KHAASAFSLWFPVEGDDYDYGKLKSEMGQRVIDKLTRIAPNFERLITRHTTFTPRHMG
                     TMFGAPGGDYCYGLLHPDQIGFNRPGPTGFRDQPIGIDGLYLGNAGCYGGPGITFSPG
                     YNAARQALART"
     gene            complement(4615..5784)
                     /locus_tag="VF874_20315"
     CDS             complement(4615..5784)
                     /locus_tag="VF874_20315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9178315.1"
                     /translation="MTVSRLTPFATTVFAEMSALAAQIGAVNLGQGFPDEDGPPAMLE
                     AARQAIADGVNQYPPGLGIAPLREAIAADRQRKYGISYDPEREVLVTVGATEAIAAAV
                     IGLVEPGSEVLLVEPYYDSYAPVLAMAQAQRTAVPLVPDGRGFALDVDALRRAVTPRT
                     KALIVNSPHNPTGTVLTEAELTAIAEVAVDADLLVITDEVYEHLVFDDLRHLPLAAYP
                     GMEDRTVTISSAAKMFNCTGWKIGWACGNADLIAAVRTAKQFITYVGGAPFQPAVALA
                     LNTQQDWVRGLRKTLQARRDRLSTGLTELGFEVHHSGGTYFLCADPRPLGYSDSTTFC
                     AELPHRAGVAAIPLSAFCDPDGEHTAAWNHLVRFTFCKKDDTLDEALRRLATLSR"
     gene            6056..7057
                     /locus_tag="VF874_20320"
     CDS             6056..7057
                     /locus_tag="VF874_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9178316.1"
                     /translation="MTAARLQLNRADMDDANRIVSAMTQAFAAKVVGQQNLRESLLIG
                     LLAGGHILLESVPGLAKTTAARVLAESVHGGFQRIQCTPDLLPSDIVGTQIYEAETHS
                     FATQLGPVHTNILLLDEINRSSAKTQSAMLEAMEERQTTIAGTVYPIPDPFLVIATQN
                     PVDQEGTYPLSEAQTDRFMLKEVLRYPSTQEEVEVMTRIDAGVYDRSRPSRPVVALDD
                     VRRLQAVVRSVHMDPVLMTYASELVAVTRNPHQFLSAQLARLIEYGASPRATIAFCTA
                     ARALAVLSGRGHVVPEDIGKLAHRVLRHRLILGFEAATAKVTTDDVVDAVLGAVRVP"
     gene            7066..7938
                     /locus_tag="VF874_20325"
     CDS             7066..7938
                     /locus_tag="VF874_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9178317.1"
                     /translation="MGKYLNHAKLHFGTNTRGLLAGGRYALLHTRTLEFDDLRPYVPG
                     DDVRDIDWKASARSGTVLTKRFVSEKHHKVLLVADAGRNMSALAPSGEVKRDVALAVM
                     GAIGLITLGRLDEIGMVYGDSRGSTNVGNRRGETHIESLLDRFYRHACGRPGASDIVT
                     QLAYVAAGHRRRLLIVVVSDEPDVDDRLSDVLQQLSGRHDVMWAMVSDMPAVASAEHR
                     IDGYDVADGKFVLGAAAADRRVLSAYRRAEHARTRQLDKLLTAYAIPYTKIRSSDEIR
                     AKIVEMTQVASRVG"
     gene            7928..8401
                     /locus_tag="VF874_20330"
     CDS             7928..8401
                     /locus_tag="VF874_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178318.1"
                     /translation="MSGDLLRYLGGPGPYSGWWLVLGVLVVLLVIAWCAGVFVWTLPS
                     STLRRIPLIRSIHARVVRRRFARSIRDVLDEYKAGEMSSTDAAAAIRRTLRGFLALET
                     SGRAYFMHVGDMAASDLAAAAPLFSVLDEAQFDTTSVVDLAGVGRDAEELIQTWS"
     gene            8401..9429
                     /locus_tag="VF874_20335"
     CDS             8401..9429
                     /locus_tag="VF874_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178319.1"
                     /translation="MWWPVYIAGLLCLCGGIALAVALRPPRFHGRVRPLAHVNRLTRL
                     PEYARAVRAQLWSTVVIILLLVGMFLAALVAGSRPLGASSRASEPDDIMLCVGAPVTD
                     PATAGLLNYFARQSLSFDTQRIGLTSPSLRVVPLTRDYRYASDSFLRYARLADLQRHG
                     PLPPPQATELKTRVSEFTRPLDYVDYARGVEDVLALCMAGFPGEKSERRRSLIYLGDS
                     SIRDADEKRSALFNAAQATQMAQAAGIQVNVLARSDASAAAADLGSIAAATGGTFDVY
                     DPAGSTADLAGSTNPTLSAALDRIRAHAPEAVGAEQNSLSDTPRLALAFGVALAALLC
                     MWPTVLRR"
     gene            9426..10415
                     /locus_tag="VF874_20340"
     CDS             9426..10415
                     /locus_tag="VF874_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9178320.1"
                     /translation="MTFNPVLPAAVLFGVAALILTSRVLAPRRASVLRWIALTLAMLL
                     LVLAAAGPALGARTSQNPSALGTVNVFFVVDRSAASRVEDFGDGASRMAGIRRDMAAV
                     VDQYPGARFAVISFASSSELDWPLSEDVWSLKPMVAALSTYTSTSPEALFEANAAAGN
                     DILRYELEQSAEQFPGSKNLVFYFGSGAAGSTAPQGNFDVGSEPVAGGAVLGYGTTAG
                     GPVPAQMVDGQVTYLVDTPGGAPVQSAIDEPRLRTIADQLELPYVHRDSGQSIPTALF
                     AVGDSQGSVSGDEKRVELYWVFSLAAAALLLVEIYLTIRRYRRNRLVRQEVEL"
     gene            10412..11170
                     /locus_tag="VF874_20345"
     CDS             10412..11170
                     /locus_tag="VF874_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178321.1"
                     /translation="MSPSRLRLRRRLLAWSAPVLVVALVVAYKLISVVVSGNGAASAL
                     TDRDVDALRDDVGTLEVFDVVESDKTAFWAANLAALEGGLAEADSRFSAMLTHTDVCP
                     VRVNLELVRETDGDLAARGGDTRRAEQRYRDALAVVDGAPQGCFEGNGDPDADRRAVR
                     NDAAARLADKINALRTPLPPAPAAPAPVVPAPPPPPSPPPVDASAALAGVDPDWLPGE
                     GPPPALRLEPGVGNPLDRLREALANSDAAAQREG"
     gene            11303..>12409
                     /locus_tag="VF874_20350"
     CDS             11303..>12409
                     /locus_tag="VF874_20350"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9178322.1"
                     /translation="MSEEAFIYEAIRTPRGKNKNGALHEVKPISLVTGLIDELRRRFP
                     DLDVNLISDVILGVVSPVGDQGSDIARTAVLAAGLPYTTPGMQLNRFCSSSLDAVNTA
                     AMKVRSGWEDLVLAGGVEMMSRVPMGSDGGAWALDPETNYTVGFVPQGIGADLIATIE
                     GFSREDVDAFALRSQQKAAAAWSGGYFAKSVVPVRDQNGLVILDHDEHMRPDTTMEGL
                     AKLKTAFDGIGAMGGFDDVALQKYHWIEKIDHVHTGGNSSGIVDGASLLLVGSEKAGE
                     SQGLTPRARIVAAAATGADPVIMLIGPTPATRKVLDRAGLTVDDIDLFELNEAFAAVV
                     LKFQKDLQIPDEKLNVNGGAIAMGHPLGATGAMIT"
BASE COUNT         2042 a         4067 c         4227 g         2073 t
ORIGIN      
        1 gaagagacag gacgccagtt acacgctcac cgaggagggc gacaagacgc gggtgaagtt
       61 cgacttgacc gtcgacccgg cgattccgat tcccggcttc gtgatgaaga gggcgatcaa
      121 gggcgcgatg aacgacgcga cggacgggtt gcgcaagcgt gtgctgtctc tgaaaaaggg
      181 cggccgttag tctgctggcg tgaccaacag cggacccctg gccggggtga gggtaatcga
      241 actgggcgga atcggaccgg gaccgcacgc ggcgatggtg ctggccgatc tcggggccga
      301 cgtggtgcgg gtgcgacgac ccggcgccac ggcgatgccg cgcgaggacc gcgatctgat
      361 gcaccgcggc aagcgggtcg tcgacctcga cgtgaagggt gagccgcaga agctgctcga
      421 cctcgcggcc aaggccgacg tgctgctgga cccgttccgg ccagggacct gcgagcgcct
      481 gggcatcggc cccgaggact gcgccgcggt caacccgcgg ctgatcttcg cccgcatgac
      541 gggctgggga caggatgggc cgatggcgtt gaccgccggg cacgacatca actacctgtc
      601 gcagaccggc gcgttgggat cgatgggcta ccgcgaccgg ccgccgctgc cgccgatgaa
      661 cctcgtcgcc gactttggtg gcggttcgat gctgttgctg ctgggcatcg tcgccgcgct
      721 gtacgaacgc gagaagtccg gcaagggtca ggtggtcgac gccgcgatgg tcgacggggt
      781 cagcatcctg gcgcagatgc agtggacgat gaaggccgtc ggcacgctgt ccgacgaacg
      841 cgagtccagc ctgctcgacg gcggcgcacc gttctaccgc acctacgaga cgtcggacgg
      901 caagtacatg gccgtcggcg ccatcgagcc gcagttctac gctcaacttc tcgacggcct
      961 cggattgtcg gccgacgacc tgcccaacca gctcgacccc gacggcttcc cgcagatgcg
     1021 caagctgttc accgagcggt tcgccagcaa gacccgcgac gggtggaccg agatctttgc
     1081 cggcacagac gcctgcgtca cgccagtgct gacgtggagt gaggccgcgg gcaacgaaca
     1141 cctcagggca cgatcgacga tcgtgcaccg caacgggttc gaccaggcag ctcccgcgcc
     1201 gcggttctcg cggacgccgg ccacagagtt cggcccgccg ccctccgaca ccacgccggt
     1261 cgaggacatc ggctggtaag aaagttagct agacgcggcg gaaagcgctt ggcaaccgca
     1321 gcgagggact acccttgact gcgtggcagt gcgcgcgtca cgtgaagtgc tgatcgatgc
     1381 tcccccggag gcgatcctgg acgcgcttgc cgatatcgag tcggcgccct cctggtcgtc
     1441 aatgcacaag cgtgtcgaga tcgtcgacaa gtacgacgat ggccggccgc accacgtgaa
     1501 ggtccattac aagctgatgg gtatcaccga caaggagctg ctcgagtacc actggggacg
     1561 cgactgggtg gtctgggacg ccgaggagac gtttcagcag catgcccagc acgtcgaata
     1621 cacgttgcga cccgaagccg gcaagacgcg ggtgcgtttc gacatcacga tggagatgcg
     1681 cgccccgatc cccaagttcc tgatgaagcg cgccaagaag tacgtcctcg acatcgcgac
     1741 cgagggcctg cggcagcagg tgagcggggg gaagtcctcg gtgcccgcga atcccgaagt
     1801 gacgtaaggc tttacggctg cagcgccgcg ggttcaggcg atgccgcagc gactcggcgc
     1861 tgacgctcta cggtcgccag gtagaacgcc cacgggaagg ctgaactggt ggtcaggatc
     1921 aagagcaggt acagccacgc gcgtcgaacc cggcggcgcc ggccgtcgac gaggaggaac
     1981 agcggcagca ggatcacgct catgatcgtg tagtcctggc tcacgttact ggcggcgggg
     2041 ttggtgtacc ccagctgaat gaattccttc cagctgccgt ggtctcccca gatcgcgttg
     2101 tgtgttccct gcgagtaatc cgtgacgtac cggtagctga agtgatagcc gagcaccgtc
     2161 gccgcgatgc ccgtgacgta aaagacgacc tcgaggacag acaccttcgg ggtcgccggc
     2221 tgcctgaaga tctgcgggtt tgacgccacg atcgcgacga tgaccgctat gccgatgacg
     2281 agatggatgg cgagcgagag catggtgccg cagtctggtc cgctccacga gttatgtcaa
     2341 tattgacata acttcctgta ccgtaccggc atggtccggc cggcgcagac cgtgcgcagt
     2401 gagcgcaccc gtgaagcgtt gcgacgcgcc gccctgctcc gcttcctcga tcagggcgtg
     2461 gaggacacgt cggccgaaca gatcgcggcg gacgccggag tctcgctgcg cacgttctac
     2521 cggcacttcg cgtccaaaca cgatctgctc ttcgcggact acgacgccgg cctgcactgg
     2581 ttccgctcgg cactggcctc ccgcccgtcg ggcgagccgc tcatcgagtc tgtccaagcg
     2641 gcaatcttcg cattcccgta cgacgttggc gtcgtgaccc agatcgcctc gctgcgcgcc
     2701 gccgagctcg aaccggatcg gatcatccgg cacatccgtc atgtcgaggc ggactttgcg
     2761 gaggcgatcg ccgagcacct tcgccgtcga agagcaaaag gagacaacct tgacggcgcg
     2821 cttcagatcg cggtcaccgc ccgatgcatt gcggcagcca tctttggagc gatggagctg
     2881 tggatggtgg ccgacgaacg gtccctcgcg gatctggcga gcatgtgcca gacggcgctc
     2941 ggctggctcg atggcgcgct ctttcgtcaa tattgacaaa actcggcggt cctgacagcg
     3001 tcctcgaatg gcagattacg acgcgatcgt ggtcggcgcg ggccacaacg gtctcaccgc
     3061 cgcggtgctg ctgcaacggg ccgggcttcg gacactgtgc ctggagtcga agctctatgc
     3121 gggtgggatg gcgtccaccg tggagttgtt cgacggctac cagttcgaga tcgccggctc
     3181 actgcagctg tcgacgtcag cggtcgtgag ccgagaactc gggctggacg cattgccgac
     3241 cgtcgacctc gacgtgatgt cggtgtcgct gcggggcgtc ggtgatgacc cggtcgtgta
     3301 ctacacggat ccgctgaaac tgctgacaca cctcaacgag gtgcacggcg ccgaggcggt
     3361 caacgggatg gccggcttga tggcatggag ccaggcaccg gcccgcgccc tgggacgctt
     3421 cgacgcctgc caactgccga agacgatgga cgagatgtat gcgtgcgcca ccaacgagtt
     3481 cgagcgggcg gcgatcaccg acatgctgtt cgggtcggtc agcgacgtgc tggacaggta
     3541 cctgcccgac agggagaagc acggggcgct gcgcgggatg ctcgcgttcc ttgccgtcaa
     3601 caccacctac cgcgggcccg aaacaccggg aaccgcagcc gctttcgcgt tcgggttggc
     3661 cgtgcccaac gaggacgcgg tgttgaccaa gaagctgcgc ggcggcatcg gcgcgctgac
     3721 ccagcacctg ctcgggctgt tcaccggtga tggcggagag ttgcggttgc gaaccccggt
     3781 cgccgagatc ctggttcacg acggatgggt tgccggggtg cggaccgagg acggctcgac
     3841 gatcagcgcg ccgctcgtgg tgtccagcgt tgcgccggat ttcacggtct tgcgcctcgt
     3901 cgacggtggc gcggtgtcct cggacgtgcg ggaacgattc tcgcgcgtcg accaccgcgg
     3961 cagctacctg cagatgcact tcgcactcga cggcatcccg gaattcgccg cgccgtacga
     4021 actgctcaac gatccgatga tgcaggccaa catcgggctg tttgccactc ccgaagaact
     4081 gcaacagcaa tgggaagact gccgtcgcgg tattgtcccc gcggatcctg cggtggcact
     4141 gcagatcccg tcggtgcacg acccggcact tgcgcccgac ggcaagcacg ccgcgtcggc
     4201 attctcactg tggttccccg tcgaaggcga cgactacgac tacggcaagc tgaaatccga
     4261 gatgggccaa cgcgtcatcg acaagctgac ccgcatcgcg ccgaacttcg agcggctgat
     4321 cacccggcac accacgttca cgccgcggca catgggcacg atgttcggcg caccgggcgg
     4381 cgactactgc tacggcctgc tgcaccccga ccagatcggc ttcaatcgtc caggtccaac
     4441 gggcttccgc gaccagccga tcggcatcga cggactgtac ctgggtaacg cgggatgcta
     4501 tgggggaccg gggattacgt tcagtcccgg ctacaacgcg gcgcgtcagg ccctggcgcg
     4561 gacgtgagga gcaattaagc cgcgcggacg tgaggagcaa ttaagcctga gaagtcaacg
     4621 gctgagcgtc gccagccggc gcagcgcctc gtcgagcgtg tcgtccttct tgcagaacgt
     4681 gaaccgcacc aggtgattcc acgccgcggt gtgctcgccg tcgggatcgc agaacgccga
     4741 cagcgggatc gcggcgacgc ccgcgcggtg tggcagctcc gcgcagaacg tggtgctgtc
     4801 ggagtagccg agcggacgcg ggtccgcgca caagaaatac gtgccgccgc tgtggtgcac
     4861 ttcgaagccg agctccgtca atcccgtcga tagccgatct cgcctcgcct gcaacgtctt
     4921 tcgcagcccg cgcacccagt cctgctgcgt gttgagcgcc agcgcgacgg cgggctggaa
     4981 cggagcaccg ccgacatagg tgatgaattg cttggccgtg cggaccgccg cgatcaggtc
     5041 ggcgttgccg cacgcccagc cgatcttcca gcccgtgcag ttgaacatct tcgcggcgct
     5101 ggagatcgtg acggtgcggt cctccatccc cgggtacgcg gccagcggga gatgacgcag
     5161 gtcgtcgaac accaggtgct cgtagacctc gtcggtgatc acgagcaggt cggcgtccac
     5221 cgccacttcg gcgatcgcgg tcaactccgc ttcggtcagc accgtgcccg tcgggttgtg
     5281 cggggagttg acgatcagcg ccttcgttcg cggcgtgacg gcgcggcgca gcgcgtccac
     5341 gtcgagtgcg aagccgcggc cgtcgggcac cagcggcacc gccgtgcgct gcgcttgagc
     5401 catcgccagc acgggcgcgt aggagtcgta gtacggctcg acgagcagga cctccgagcc
     5461 cggctcgacg aggccgatca ccgcagcggc gatcgcctcg gtggcgccga ccgtgaccag
     5521 cacctcgcgc tcggggtcgt agctgatgcc gtacttgcgc tgccggtcgg cggcgatggc
     5581 ttcccgcagc ggcgcgatgc ccagcccggg cgggtactgg ttgacaccgt cggcgatggc
     5641 ctgccgcgcg gcctccagca tcgccggcgg gccgtcctcg tcgggaaacc cctgcccgag
     5701 gttgacggcc ccgatctgcg cggccagcgc cgacatctcg gcgaacaccg tggtggcgaa
     5761 gggcgtcagc cgcgagaccg tcatagccgc ctatcttgcc tgtcgctcct cgccttccgc
     5821 ccgcgcaaat tgcatcgaca ctgcgttttt acgggacacg ccggtcaggc ggacgaaaac
     5881 gcagagtcgg cgacgccacg ctcaggtccg cgctcgacga cgcccctaga atgggtggcg
     5941 tcccgctggt cggcgtgagg cgcggccggc ggatatcacg tttggcgccc cgtttggtgc
     6001 gggcatgatt cccagcagcg ttgcattgcg cgcgccaacg acgaaaaagg tatcgatgac
     6061 tgcagcacgc ttacaactga atcgcgccga catggacgac gccaatcgca tcgtcagcgc
     6121 aatgacacag gcgttcgccg ccaaggtcgt cgggcagcag aacctgcggg aatcgctgct
     6181 gatcggcctg ctggccggcg ggcacatcct gctggagagc gtgccgggtc tcgcgaagac
     6241 gaccgcggcc cgcgtgctgg cggagtcggt gcacggtggc ttccagcgga tccaatgcac
     6301 accggacctg ctgcccagcg acatcgtcgg cacccagatc tacgaggcgg agacgcactc
     6361 gttcgccacc cagctcggcc cggtgcacac caacatcctg ttgctcgacg agatcaaccg
     6421 ctccagcgcc aagacccaga gcgcgatgtt ggaggcgatg gaggagcgtc agaccacgat
     6481 cgcgggcacg gtgtatccga ttcccgaccc gttcctggtg atcgccaccc agaatccggt
     6541 cgaccaggag ggcacgtacc cgctgtcgga ggcccaaacc gaccgcttca tgctcaagga
     6601 ggtgctgcgc tacccctcca cgcaggaaga ggtggaggtg atgactcgca tcgacgccgg
     6661 cgtctacgac aggtcccggc ccagccgccc ggtggtcgcg ctcgatgacg tccgccggct
     6721 gcaagccgtg gtgcgcagcg tgcacatgga cccggtgctg atgacctacg ccagcgagct
     6781 cgtcgccgtc acccgcaacc cgcaccagtt cctctccgcg cagctggcgc ggctgatcga
     6841 gtacggcgcc agcccccgcg cgacgattgc attctgcacg gccgcgcgtg cgcttgcggt
     6901 gctgtcgggc cgcggtcacg tggttcccga ggacatcggc aagctcgctc accgggtgct
     6961 gcggcaccgg ctgatcctcg ggttcgaggc cgccaccgcg aaagtgacca ccgacgacgt
     7021 ggtcgacgcg gtgctgggtg cggtgcgggt tccgtaggga gccccatggg caagtacctc
     7081 aatcacgcca agctgcactt cggcaccaac acgcgcgggt tgcttgcagg cggccgctac
     7141 gcgctgctgc acacccggac gctggagttc gacgatctgc ggccctacgt gcccggagac
     7201 gacgtgcgag acatcgactg gaaggcgtcg gctcgctcgg gcacggtgct gaccaagcgg
     7261 ttcgtgtccg agaaacacca caaggtgctg ctggtcgcgg atgccggccg caacatgtcg
     7321 gcgctggcgc cgagcggaga agtcaagcgc gacgtcgctc tcgcggtgat gggcgcgatc
     7381 gggctgatca cgttgggccg cctcgacgag atcggcatgg tctacggcga ctcgcgcggg
     7441 tccaccaacg tcggtaaccg acggggcgag acgcacatcg aaagcctgct ggaccggttc
     7501 tatcggcacg cctgcgggcg gccgggcgcc agcgacatcg tcacgcaatt ggcgtatgtg
     7561 gctgccgggc accggcgccg gttgctgatc gtggtcgtgt ccgacgaacc cgacgtcgac
     7621 gaccggctgt ccgatgtgct ccaacaactt tcgggccggc atgacgtgat gtgggcgatg
     7681 gtgtcggaca tgcctgccgt cgcttcggcc gagcatcgaa tcgacggcta cgacgtcgcc
     7741 gacggcaagt tcgtgctcgg cgccgcggct gcggatcgcc gggtgctctc ggcgtatcgc
     7801 cgagccgagc atgcccgcac gcggcaactc gacaagctcc tcaccgcata cgcgatcccg
     7861 tacacgaaaa ttcggagcag tgacgagatt cgcgcgaaga tcgtcgaaat gacccaggtg
     7921 gcaagccgtg tcgggtgact tgctgcggta cctcggtggt cccgggccgt actccggctg
     7981 gtggctggtc ctcggtgtgc tggtcgtgtt gctcgtgatc gcttggtgcg cgggagtttt
     8041 cgtgtggact ctgccctcgt cgacgctgcg ccggattccg ctgatccggt ccattcatgc
     8101 cagggtggtt cgtcgccggt tcgcgcggtc catccgcgac gtgctggacg agtacaaggc
     8161 gggtgaaatg tcgtcgacgg atgcggccgc cgcaatcagg cgcacgctgc gcggcttttt
     8221 ggcgctggaa accagtggcc gagcctattt catgcatgtc ggcgacatgg ccgcaagcga
     8281 tctggcggcg gctgcgccgc tgttctcggt gctcgacgag gcgcagttcg acacgacgtc
     8341 cgtcgtggac ctcgccggtg tcggacgcga tgccgaggag ctgatccaga cgtggagttg
     8401 atgtggtggc cggtctacat cgccggattg ctctgcctgt gcgggggtat cgcgttggcg
     8461 gtggcgctgc gtccgccgcg gtttcacggt cgggtgcggc cactggcgca cgtcaaccgg
     8521 ctcacccggc tgcccgagta cgcacgcgcg gttcgcgcgc aactctggtc gacggttgtg
     8581 atcatcctgc tgctggtcgg tatgttcctg gccgcactgg tggccggctc gcggcccctt
     8641 ggcgcgtcga gtcgcgcgtc tgagccggac gacatcatgc tgtgtgtcgg tgcgcccgtc
     8701 accgaccccg ccaccgcggg tctgctcaac tactttgcgc gacaatcgtt gtccttcgac
     8761 actcagcgca tcggcttgac gtcgcccagc ctgcgtgtcg tgccgttgac ccgtgactac
     8821 cggtacgcca gcgattcctt cctgcgctac gcgagactcg ctgatctaca gcgccatggc
     8881 ccgctaccgc caccgcaggc caccgaacta aagacccggg tcagtgaatt caccagaccg
     8941 ctcgattacg tcgactacgc gcgcggcgtc gaggacgtcc tcgcgttgtg tatggcgggt
     9001 tttcccggcg agaaatccga gcgtcggcgt tcgctgatat atctgggcga cagcagcatt
     9061 cgtgacgccg acgagaaacg ctcggcgctg ttcaatgccg cacaagcgac gcagatggcg
     9121 caggcggccg gaattcaggt caatgtcctg gcacgctcgg acgcttcggc agccgccgcc
     9181 gatctcgggt cgatcgctgc cgccaccggt gggacgttcg acgtctacga cccggcgggg
     9241 tccacggccg acctcgcggg cagcaccaac ccgacgctga gcgcagcgct cgacagaatc
     9301 cgtgcgcacg cacctgaagc cgttggagcc gagcaaaact cgttgtccga cactccgagg
     9361 ctggccctgg ctttcggcgt ggcccttgct gcgctgctgt gcatgtggcc gacggtgctg
     9421 cgacgatgac gttcaatcct gttcttcccg cggcggtctt gttcggggtg gcggcgctga
     9481 tcctgacgtc gcgggtgctg gcgccgcgcc gggcatcggt gctccgctgg atcgcgctga
     9541 cgttggcgat gctgctgctg gtgttagccg cagccggtcc cgctctcggg gcccgtacgt
     9601 cgcagaaccc ttcggccctc ggcacggtga atgtgttctt cgttgtcgac cggtccgctg
     9661 catcgcgcgt cgaggacttc ggtgacgggg cgtcgcggat ggccggcatc cggcgtgaca
     9721 tggctgcggt cgtcgaccag tacccaggtg cgcgcttcgc ggtgatctcg ttcgcgtcga
     9781 gctctgagct ggactggcca ctgtcagagg atgtttggag cctcaagccg atggtcgcag
     9841 cgctctccac gtacacgtcg acgtcgcccg aagcgctttt cgaggcgaac gcggcagccg
     9901 ggaacgacat cctgcgctac gagctggagc agtctgctga gcagttcccg gggtcgaaaa
     9961 acctggtgtt ctacttcggc tcgggtgccg ctggatcgac tgcgccgcaa ggtaacttcg
    10021 atgtcggttc tgaacccgtg gcgggcggcg cggtgctcgg ctacggcaca accgcgggag
    10081 gcccggtccc tgctcagatg gtcgacggcc aggtcaccta cctggtcgac acgccgggcg
    10141 gcgctccggt gcagtccgcc atagacgaac cgcgcctgag gacgatagcc gatcagctcg
    10201 agctccccta tgtccatcgg gatagcggcc agagcatccc cacggcgctc ttcgcagtcg
    10261 gtgattcgca gggttcggtg tcgggtgacg agaaacgcgt cgagctgtat tgggtgttct
    10321 cgctcgcagc agccgctctt ttgctggttg agatctacct gacaatccgc cggtaccggc
    10381 gtaatcgctt ggtgcgacaa gaggttgagc tgtgagcccg tcccggcttc gtttgcgacg
    10441 gcgattgctg gcgtggtcgg cgccggtgct cgtcgtcgcg ttggtggtcg cctacaagtt
    10501 gatctccgtc gtggtcagtg gcaatggggc agcgtcggcg ttgacggacc gcgacgtcga
    10561 cgcgctgcgc gacgacgtcg gcacgctgga ggtcttcgac gtcgtcgagt ctgacaagac
    10621 ggcgttctgg gcagcgaacc tcgctgcgct ggaaggcggt ctggccgagg cggattcacg
    10681 attcagcgcg atgctgacgc acaccgatgt ctgtccggtg cgagtcaacc tcgagctcgt
    10741 ccgcgagaca gacggcgatc tcgctgcccg tggcggcgat acccgtcgcg ccgaacagcg
    10801 gtatcgcgac gcgctggcgg tggtggacgg cgcgccgcag gggtgcttcg aagggaacgg
    10861 cgatcctgac gccgatcggc gggcggtccg aaacgatgcc gccgctcggc tggccgacaa
    10921 gatcaacgcg ctgcgcacac ccttgccgcc cgcgccggcc gcacccgcgc cggtcgtccc
    10981 cgcgcccccg ccgccacctt cgccgccgcc ggtcgacgcg tcggcggcgc tcgctggcgt
    11041 cgaccccgac tggctgcccg gcgagggacc gccgcccgcg ttgcgtctcg aaccgggagt
    11101 gggcaacccg ctcgaccggc tccgggaggc actggcgaat tccgacgcag cagcgcagcg
    11161 agagggctag gggccgcgac cgcccaggcg caaccaacag gttggattag gccactgtta
    11221 ggctccgaac acagggtcta cgctctaaca cagacgggct gtccccagca ccgccttcgt
    11281 gttaactgaa gaggaattcg ctatgtccga agaagccttc atctatgagg ccatccgcac
    11341 accgcgcggc aagaacaaga acggcgcgct gcacgaagtc aagccgatca gcctggtgac
    11401 cggcctgatc gacgaacttc gccgccgctt ccccgacctg gacgtgaacc tgatcagcga
    11461 cgtgatcctc ggcgtcgtct cgccggtggg cgatcagggc agcgacatcg cgcgcaccgc
    11521 ggtactcgct gcgggcctgc cgtacaccac ccccggcatg cagctcaacc ggttctgctc
    11581 gtcgagcctg gatgccgtga acaccgccgc gatgaaggtg cgctccggct gggaggatct
    11641 ggtgctggcc ggcggtgtcg agatgatgag ccgggttccg atgggctccg acggtggcgc
    11701 ctgggcgctc gaccccgaaa ccaactacac ggtcgggttc gtcccgcagg gcatcggtgc
    11761 cgatctgatc gccaccatcg agggcttctc ccgcgaggac gtcgacgcgt tcgcactgcg
    11821 gtcgcaacag aaggcggccg cggcgtggtc cggcgggtac ttcgccaagt cggtggtgcc
    11881 ggtgcgtgac cagaacggcc tggtgatcct cgaccacgac gagcacatgc gcccggacac
    11941 cacgatggag ggcttggcca agctgaagac ggcgttcgac gggatcggcg cgatgggcgg
    12001 cttcgacgac gtggcgctgc agaagtacca ctggatcgag aagatcgacc acgtgcacac
    12061 cggcggcaac agctccggaa tcgtcgacgg tgcctcgctc ttgctcgtcg ggtccgaaaa
    12121 ggccggcgag tcacagggtt tgacgccgcg ggcgcgcatc gtggcggccg ccgccaccgg
    12181 cgcggatccg gtgatcatgc tcatcggtcc caccccggcg acccgtaagg tgctcgaccg
    12241 ggccgggctg accgtcgacg acatcgacct gttcgagctc aacgaggcgt tcgcggcggt
    12301 ggtgctgaag ttccagaagg acctgcagat ccccgacgag aagctcaacg tcaacggcgg
    12361 cgcgatcgcg atgggtcacc cgctgggtgc caccggcgcg atgatcacc
//
LOCUS       DASWBF010000543         4309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_120952,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000543 DASWBF010000000
VERSION     DASWBF010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120952"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VF874_20355"
     CDS             complement(<1..351)
                     /locus_tag="VF874_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEX9178323.1"
                     /translation="MAQVVPYQTAHGKRYRVRYRTPDRRQTDKRGFRTKREAELFAAT
                     VEVSKAKGEYVAPSLGRVTVGELAADWLARKQQAIAPSHYRMLDSAWRVHVARRWGSV
                     SVSDIDQLGVEAWIT"
     gene            complement(473..563)
                     /locus_tag="VF874_20360"
     tRNA            complement(473..563)
                     /locus_tag="VF874_20360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(527..529),aa:Ser,seq:cga)
     gene            complement(632..1108)
                     /locus_tag="VF874_20365"
     CDS             complement(632..1108)
                     /locus_tag="VF874_20365"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX9178324.1"
                     /translation="MTTWNSVSAVGDEDLIRVALQAAATAGPRDVPVGAAVFSADGVE
                     LARAVNAREALGDPTAHAEILALRAAASVLGDGWRLVGTTLAVTVEPCTMCAGAAVMA
                     RVARLVFGAWEPKTGAVGSLWDVVRDRRLTHRPEVRGGVLADECAAPLAEFFARQR"
     gene            complement(1156..1677)
                     /locus_tag="VF874_20370"
     CDS             complement(1156..1677)
                     /locus_tag="VF874_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HEX9178325.1"
                     /translation="MGPQRGPAKEQAPEIPEGFAVAVVREDGKWRCSPMRAGVLTSLS
                     AAETELRELRSTGAVFGLLDVDDEFFIIVRPAPSGTRLFLSDATAALDYEIAAEALEK
                     LDADIEYEELDPDEPFEEGDLHVLSDLGLPDDVLRVILSDTDLYADEQLGMIAARMGF
                     PEEFSAALERLNR"
     gene            1762..2721
                     /locus_tag="VF874_20375"
     CDS             1762..2721
                     /locus_tag="VF874_20375"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEX9178326.1"
                     /translation="MTKTPVCVLGLGLIGGSIMRAAVKAGREVFGYNRSVEAATAARF
                     DGFDATHHLDEALRRAADTNALIVLAAPSPALPILLPYVSDMAPDNPLTDVASVKVAV
                     LQEVQKVGLEANFVGGHPMAGTAHSGWAAGNARMFMGAPWVVSVDDHVDTRCWAMVTE
                     LALDCGSVVVPARSDEHDAAAAAISHLPHLVAEAIAATAGEVPLAFALAAGSFRDVTR
                     VAATTPELVRAMCEANAEQVLPVLDRTIELLTHASNALAQHGTVADLVDDGHAARTRY
                     DGFSRYEIVGIVVGEENWREELAAAGRAGGVIRSVSPVRGSRG"
     gene            complement(2684..3199)
                     /locus_tag="VF874_20380"
     CDS             complement(2684..3199)
                     /locus_tag="VF874_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEX9178327.1"
                     /translation="MESARVQLSGNRIKAHGRIVAAATNAHPAFSASYDLVTDESGAT
                     KRLSMTVTLAERDRQLSIARDDENMWLVTDYRGQSRGAFDGALDVDILFSPFFNALPI
                     RRTGVYKDRDTVTVPVVYVTLPEFAVNPASITYSAGSDGIKVHSPVAETTVTVDDNGF
                     ILDYPGLATRI"
     gene            complement(3282..4073)
                     /locus_tag="VF874_20385"
     CDS             complement(3282..4073)
                     /locus_tag="VF874_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9178328.1"
                     /translation="MNFLQQAWSYIFTAANWGGSAGLLARTGEHLQYTVVAVAVSALI
                     AVPIGMVVGHTGRGTFVVVTGVNALRALPTLGVLLLAVLLWGLGLLPPTVALMLLGIP
                     PLLAGTYAGIANVEQAVVDAARAMGMTETRVLLRVELPNALPLILGGLRTATLQIVAT
                     ATVAAYASLGGLGRYLIDGIKVRQFYIALVGALMVTALALILDALLAFAVWASVPGTG
                     RLRRSRIAGDIRPSMPEPLVDDVAAVTTMRRTSRSARARGTRRLP"
     gene            complement(4070..>4309)
                     /locus_tag="VF874_20390"
     CDS             complement(4070..>4309)
                     /locus_tag="VF874_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9178329.1"
                     /translation="VAGLRVVAVTNISMVSVGSVIGIGGLGTWFTEGYQADKSDQIIA
                     GIIAIFVLAIVVDSAILVAGRLATPWARARTGGAR"
BASE COUNT          713 a         1532 c         1397 g          667 t
ORIGIN      
        1 cgtgatccac gcctccacgc cgagctggtc gatgtcggag acgctcaccg agccccagcg
       61 tcgggcgacg tgcacgcgcc aggcactgtc gagcatccgg tagtgcgagg gcgcgatggc
      121 ctgctgcttg cgcgccagcc agtcggctgc cagctcgccc accgtcaccc ggcccagcga
      181 gggcgcaacg tactcgcctt tggcctttga gacctccacc gtcgcggcga acagctcggc
      241 ctcgcgcttg gtgcggaaac cgcgcttgtc ggtctgccga cggtcgggcg tgcgatagcg
      301 gacgcggtat ctcttgccgt gggcggtctg gtacgggaca acctgagcca tggcccagac
      361 cataggccgc aaccgtgacg gaatgtgggc agaatgtggg caaacccgaa gaacgggcgg
      421 tcacagtaat cgctaattat gccatctagc tgcgctaaca ggaggatggt tatggcggtg
      481 gcggagggat ttgaaccctc ggacgggggt tacccgtcac acgctttcga ggcgtgctcc
      541 ttaggccgct cggacacgcc accgcgggtc agcttaccgg ccgacccgaa cgctcattca
      601 cgctgccctt ggctcttcgc gcaagcgctc atcaccgttg ccgcgcgaag aactccgcca
      661 agggcgcagc acactcatcg gcgagcactc ccccacgcac ctccgggcgg tgcgtgagcc
      721 ggcggtcgcg gaccacgtcc cacagtgagc cgaccgcgcc cgtcttcggc tcccacgccc
      781 cgaacaccag ccgcgccacc cgcgccatca ccgccgcgcc cgcgcacatc gtgcacggct
      841 ccaccgtcac cgccagcgtg gtgccgacca gccgccagcc gtcacccaac acggacgccg
      901 ccgcccgcag cgcgaggatt tctgcgtgcg cggtcgggtc gccaagcgcc tcccgcgcgt
      961 tgaccgcccg cgccagttcg accccgtcgg cgctgaacac cgcggcgccc accggcacgt
     1021 cgcggggccc ggccgtggct gccgcctgca gggctacgcg gatgaggtct tcgtcaccga
     1081 ccgccgaaac cgaattccag gtcgtcatct cgccgatttc acaaccgtgg cttcattttc
     1141 gccgcgcggc gaatatcacc ggttgaggcg ctcgagcgcc gccgagaact cctcggggaa
     1201 gcccatccgc gccgcgatca tgcccagctg ctcgtcggcg tacaggtccg tgtcggagag
     1261 gatcaccctg agcacatcgt ccgggagacc gagatcggac agcacgtgca gatcgccctc
     1321 ctcaaacggc tcgtcggggt cgagttcctc gtactcgatg tccgcgtcga gcttctccag
     1381 ggcttccgcg gcgatctcgt agtcgagcgc tgcggtggcg tctgagagaa acaaccgcgt
     1441 acccgacggt gccggacgca cgatgatgaa gaactcgtcg tccacatcga gtaatccgaa
     1501 aactgctccg gtacttcgca attcgcgtaa ctcggtctcg gcggcgctga ggctggtcag
     1561 cacgccggcg cgcatcggcg agcagcgcca cttgccgtcc tcccggacca cggccaccgc
     1621 gaagccctcc gggatctccg gagcctgttc tttggccggt ccacgctgtg gtcccatggc
     1681 cgcatacggt agtcgcccag cctgggcctt gaccacgtat tgagggcaga cgccccgggg
     1741 cgaatgtgcc aaccttgaca ggtgaccaag acaccggtgt gtgtgctcgg cctcggactc
     1801 atcggggggt cgatcatgcg cgccgcggtg aaggccggcc gcgaggtgtt cggctacaac
     1861 cgctccgtcg aggcggccac ggccgcccgt ttcgacggct tcgacgcgac acatcacctc
     1921 gacgaggcgc tgcgccgcgc ggcggacacc aacgcattga tcgtgctggc cgctccctca
     1981 cccgcactgc cgatcttgct gccttatgtc agcgacatgg cgcccgacaa tccgctcacg
     2041 gacgtcgcca gcgtcaaagt ggcggtgctt caggaggttc agaaggtcgg cttggaggcg
     2101 aacttcgtcg gcggacatcc aatggcgggc accgcacact ccggttgggc cgccggcaac
     2161 gcccgaatgt tcatgggagc gccgtgggtg gtcagcgtcg acgaccacgt cgacacgcgg
     2221 tgttgggcga tggtcacaga actggcactg gactgcggct ccgttgtggt gccggcccgg
     2281 tccgacgaac acgacgcggc cgccgcggcg atctcacatc tgccgcacct ggtcgctgag
     2341 gccatcgcgg caaccgccgg cgaggtgcct ctggcgttcg cgttggccgc cgggtcgttc
     2401 cgggacgtca cccgcgtcgc cgccaccacc ccggaactgg tccgggccat gtgcgaggcc
     2461 aacgccgagc aggtgctgcc cgtcctcgac cgcacgatcg aactgctcac ccacgcctcg
     2521 aacgcgctcg ctcagcacgg tacggtggcc gacctggtcg acgacggcca cgcggcccgg
     2581 actcgctacg acggcttttc gcgctacgag atcgtcggca tcgtcgtcgg cgaggagaac
     2641 tggcgcgagg agcttgcggc ggcgggcaga gccggcggcg tgatcagatc cgtgtcgcca
     2701 gtccggggta gtcgaggatg aaaccgttgt cgtcgacggt gaccgtggtc tcggcgaccg
     2761 gcgagtgcac cttgatccca tcggatccgg cgctgtaagt gatcgacgcc gggttcaccg
     2821 cgaactccgg caatgtcacg tacaccaccg gcaccgtcac cgtgtctctg tccttgtaga
     2881 ctccggtccg tcgaataggc agcgcattga agaacgggct gaacagaata tccacatcca
     2941 gcgcgccgtc gaaggcgccc cgcgactgcc cccggtagtc ggtgaccaac cacatgttct
     3001 cgtcatcgcg cgcgatcgac agctggcggt cccgctcggc cagcgtcacg gtcatcgaca
     3061 accgcttggt cgcgccagat tcgtcggtga ccagatcgta agacgcgcta aacgccgggt
     3121 gcgcgttggt ggcggcggcc acaatccgcc cgtgggcctt gatccgattg ccggacaatt
     3181 gaactcgcgc cgactccatg cgcgcaacat cataggcgcg ccaagtcaag attgccggcc
     3241 atgcgcttgg tgtcgcttca gaagggctcg cactcacccg tctacggtaa gcgacgggtg
     3301 ccccgcgcgc gtgcggaccg ggaagtgcgt cgcatcgttg tgacggccgc cacgtcatcg
     3361 accaacggct ctggcatgct cggcctgatg tcgccggcta tgcggctccg gcgcagccgg
     3421 ccggtgcccg gcacggaagc ccacaccgcg aaggccagca gtgcatcgag gatcaacgcc
     3481 agcgccgtga ccatcaacgc acccaccaag gcgatgtaga actggcgcac cttgatgccg
     3541 tcgatcaggt atcggccgag tccgccgagg ctcgcatacg ccgcgaccgt ggccgtcgcg
     3601 acgatctgca gtgtcgcggt gcgcaggccg ccgaggatca gcggcagcgc gttcggcagc
     3661 tcgacgcgca gcagcacccg tgtctcggtc atgcccatcg cccgcgcggc gtcgaccacg
     3721 gcctgctcca cgttcgcgat gccggcgtat gtgccggcca gcagcggtgg gatgccgagc
     3781 agcatcagcg ccaccgtggg cggcagcagg cccagtcccc acagcagcac agcgagcagc
     3841 agcaccccga gcgtcggtag tgcgcgcagc gcgttgacac cggtgaccac gacgaacgtg
     3901 ccccgcccgg tgtgcccgac caccatgccg atcggcacgg cgatcaacgc cgagaccgca
     3961 actgccacca cggtgtactg caggtgctcg ccggtgcgtg ccagcagtcc ggcgctgccg
     4021 ccccagttgg cggcggtgaa gatgtaggac cacgcctgct ggaggaagtt catcgcgcac
     4081 cgcccgttcg tgcgcgcgcc cacggcgtcg cgagccggcc ggccaccaag atcgcgctgt
     4141 cgacgacgat cgcgagcacg aagatcgcga tgatcccggc gatgatctgg tcgctcttgt
     4201 cggcttgata accctcggtg aaccaggtgc ccaggccgcc gatgccgatc accgaaccga
     4261 ccgacaccat cgagatgttg gtgaccgcga ccacgcgcag gccggccac
//
LOCUS       DASWBF010000544         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_455869,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000544 DASWBF010000000
VERSION     DASWBF010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455869"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..1565)
                     /locus_tag="VF874_20395"
                     /pseudo
     CDS             complement(330..1565)
                     /locus_tag="VF874_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
     gene            complement(2011..2832)
                     /locus_tag="VF874_20400"
     CDS             complement(2011..2832)
                     /locus_tag="VF874_20400"
                     /inference="COORDINATES: protein motif:HMM:NF013265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEX9178330.1"
                     /translation="MGARHIAVFVSAATVASWAPLSAPVPSASAQPCPDVEVVFARGT
                     TEDPGLGPTGQAFVDALRARVGTKSIGVYPVDYPASTDFRTALDGINDASAHVRSVAA
                     TCPNTELVLGGFSPGAAVMGFVTANAIPDGAPEGVPNPMPPDVADHVAAVALFGKPNY
                     RFMRAINEPQIAVGPLYAAKTIDLCVPDDLICSSGMDFNAHMQYAETGMVDQAATFTA
                     SHLLVNSGPAAPPHSPAPAAPPHSPAPATPQPPRPQLAPGTLLMCTTVCRVLGPA"
     gene            complement(3053..>3456)
                     /locus_tag="VF874_20405"
     CDS             complement(3053..>3456)
                     /locus_tag="VF874_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178331.1"
                     /translation="ATAAAERVDGVHHGGGGADRRDEPDPPRPPLPVGWFGVAGRNVV
                     YYLLASAVFRDGISGVFAFGGVLGARVYWISQADVLLFGVAASVVAALGAVAGGLLDD
                     HVGSKPVIAASLSAMVTIGLVQPACAAPRVR"
BASE COUNT          600 a         1119 c         1170 g          567 t
ORIGIN      
        1 gccggattcc tgcggcgttc gcgccgcccg gcacatctgg atgcgtgcca gcttgaatcc
       61 ggctggcccc ggcaagcgag cgtctgacgc tcctgcgtaa cggagtttcg gtcatagacc
      121 ggaactgatg agttcgtggt aggtcgatca agagcaccct agcctcgatt tttcgcgcgg
      181 cgttgtcggg tcggatgatg ccggcgcaag gcgggccggt gggcttgatt ttgctggatg
      241 ggtatgtcgg gtcagccggt gtcgctcggt gggcgggctt tccagggccg gcggggttcc
      301 tcgggtcggg tggacggtgg cctggacggt caggggaagg cggcccgaag tcgttgccat
      361 gcttggcaaa tcgcggctgc ccaccgccag gtggcgtcga tgcgtagccg gatttgtcgg
      421 gcgcctcggg tgattcgtgc cgcgacgtgc aggacccggt atcggaaggt gccgatctca
      481 caggtggcca gatcgggatg ctcggcgaag ccgatcagtt tggcccagac caccaaatct
      541 gttgccgcca agatgatttc cagccaggct gcgttcgcgt cggcgcggtg acacggcagg
      601 ttggccagcc cggtggcctt cgcctggcgg atccggtcct ccacccgggc gtgctggcgg
      661 tggcgcagtt ccagcccgcc gagttggccg gggaccacac cgggtggcgt gtcggtgatg
      721 aacgcggtca ctcgcggtcc ctcggagtcg gtgaatcgca gctgcgctcc cggatgcggg
      781 cgttctgtac gcaggatcaa ccgggtcccg gccggccagc tgctcagatc caccaggtcg
      841 gtggcttcgg cgacccaggc gccgtcgcgg atggcgccgc catcctcgat cgccggatac
      901 cagccgtcgg cggtgttgag aacttccacc gcatcctgca cgcgggcatc cacggcgtag
      961 ccgaaggaat aacccacccc gcgcgcgcgg cacgtctcgg cgaacccgtg ggtggcgccg
     1021 gccgaatcgc agcgcaccag aatctgttgc gcgccgggat cgtcggggtc gggccggtag
     1081 gggcccggca gcgactccag cgcccacccc agcaccgtga tgtggtcggc ggcggtattg
     1141 ctgccggcat tcccgggtcg cagcagcccg gccaacgctt ccccatcggc gatctcggga
     1201 cggtccaaaa acgccagcag cgagtgatga ccccaggtct tcttccaggt cgcggccgcg
     1261 ccttccttgt tgtcggaatg gtcgatcgtg atggtggcat cgatatcgat atgcagccac
     1321 tcacccggcg ccggcgccgc cccgccagcc cacgccgccg cacgggcgtg cgcgcgggcg
     1381 gctcggatac cgggcaggtg tgccgcgtcg atgcgggcat cgaccagccg ccacatcgtg
     1441 gtcgtggacg ccgcggcgcc aaaggcgtgt tcccgatccc cgcaccgctg cccgacgccg
     1501 tcgatgcagt ccgccccggc ggccaccgcc gcggccagat cggcgaacac cgcacccggc
     1561 tcatacggca tcgtcaagtt gttgaccttg tgtggtggct gcgaggattg gtcgttgtgg
     1621 cgattgcggc ctctagactc ggcgcggcgg ggtgaggtcg ccgaggagct gcggaaggtg
     1681 gccgaaacgt tcgccgacgt gcctgatggt aagacagcct catatgtgct gggcgtactc
     1741 gcccacctgc tcgaccctgg ttgacctgca cgacgtgttc cgaaatcggt cggttacggg
     1801 acaggggccg gattacctcg tggcggttgt gttgccagat ctattgcggc acaacatgaa
     1861 tatggcctac cccaacctgt ctaccagcac gaacaggtta gccgccacgg tccgcaaacg
     1921 ccagggatgg actgtgaaat ccgtcggcgt atgcctacgc gggtccaaat cctgcacctg
     1981 ccaccgcagt tcagacggcg ccgcaccttg tcaagcgggt ccgaggacac gacatacggt
     2041 cgtacacatc aacaacgtcc ccggagcaag ctgtggacgt ggcggttgcg gtgtggcagg
     2101 agcaggcgag tgcggcggtg cggcaggagc aggcgagtgc ggcggtgcgg cagggccaga
     2161 atttaccaag aggtgactcg cggtgaaggt cgctgcctgg tcaaccatcc cggtctccgc
     2221 gtactgcatg tgcgcgttga agtccattcc gctagagcaa attaggtcat cggggacgca
     2281 caagtcgatg gtcttagccg catacagcgg gccgacggcg atctgcggct cattgatcgc
     2341 acgcataaac cgataatttg gttttccgaa cagggcgacc gcggcgacgt ggtctgccac
     2401 atcgggcggc atcgggttcg gtacaccttc aggtgcccca tccgggatgg cattcgcggt
     2461 gacaaaaccc atcacggccg cgcctgggga aaatccgccg agcaccaact cggtgttggg
     2521 acaagtcgcc gccacggacc ggacatgagc gctcgcgtca ttaataccgt ctaaggcggt
     2581 gcggaagtcg gtgctggcgg ggtagtcgac cggatatacc ccgatcgact ttgtgccgac
     2641 tcgcgcgcgc agcgcgtcca caaatgcctg tccggttgga ccaagaccgg ggtcctcggt
     2701 ggtgccgcgc gcgaatacca cctcgacgtc ggggcacggc tgcgcggacg cggacggaac
     2761 gggtgcgctc agcggcgccc aacttgctac cgtcgcagca ctaacgaaca cagcgatatg
     2821 gcgtgccccc acggcgcaat gctgacatac cgcgacgacc gcgacgaccg gaaaaacaaa
     2881 cggccaatga acgccgcgtg aatccgcgcc cagaggcgta tcagcgcatc atgccattag
     2941 cccgggcgcg tgcagccacg gcggcgccat agcagcaaag atagctggga cgacaactct
     3001 gatcccagca atatcccagc ggcatcgtca agttgttgac cttgtgtggt ggctagcgca
     3061 cgcgcggcgc agcgcatgcg ggctgaacca gaccgatggt gaccatcgcg ctgagcgaag
     3121 ccgcgatgac cggtttggac ccgacgtggt cgtcgagcag accgccagcg accgcgccca
     3181 acgccgccac tacgctggcg gccacgccga acagcaagac gtcggcctgc gagatccaat
     3241 acaccctggc cccgagcaca ccgccgaacg cgaacacgcc ggatatccca tcccgaaata
     3301 ccgcgctggc aagcaggtag tagacgacgt tgcggccagc aacaccaaac cagcccacag
     3361 gtagcggggg tcttggcgga tcaggctcat cgcggcggtc agcgccaccg ccgccatggt
     3421 gaacgccgtc aacgcgttcc gccgccgctg tggcga
//
LOCUS       DASWBF010000545         8226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_181753,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000545 DASWBF010000000
VERSION     DASWBF010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181753"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VF874_20410"
     CDS             <1..464
                     /locus_tag="VF874_20410"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9178332.1"
                     /translation="ENLELLQGDIAQMDLATLGQFDYIVCHGVYSWVPDNVQDAILAA
                     FRTLLVPDGVAYISYNVYPGWKAKEIVRDAMLMRSGGRETPGERVAYARGMITFLEDV
                     APADSVLARALADYKVIAASSKDYYLLHEELETFNSPCYFLDTVWPIWRRR"
     gene            419..1309
                     /locus_tag="VF874_20415"
     CDS             419..1309
                     /locus_tag="VF874_20415"
                     /inference="COORDINATES: protein motif:HMM:NF045154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEX9178333.1"
                     /translation="MLFPRHRLAYLAEALTSIMFPMNYGDKVAEPLLKECGHSQVLME
                     QYLDFVVNRPFRQTLLVHAERAQQIRYQLDRSRYERLQFGAWQPAVGGETRLDDSSQE
                     YGDPAAATVVTQNPAVKAALDALTARWPSTQSRQELLNEVQTQLRAAGADATNVEGSI
                     DDLLDFLIVRGQARYRLDPVMVEPVSTPLRLDEPIRQMAQLTRADADASTFNVWHETV
                     ALSPVDSHVLPLLDGTRDRDALEDALLALAREGQLSFERDGQPVSGESELREAVVAYI
                     DALPQRLTEMKLVSARQAGL"
     gene            complement(1294..2142)
                     /locus_tag="VF874_20420"
     CDS             complement(1294..2142)
                     /locus_tag="VF874_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9178334.1"
                     /translation="MPQVTLKQATIDYHVLGPDDSPHPPVLFVHGILVDHRLWHEVAE
                     QLAGKGFRCILPDWPLGSHRIPVNAGADLSPRGVATMIRDFIVALDLSDVTLVGNDTG
                     GGLCQLVIDAHPEHVGRLVLTNCDAFDTFPPFPFDAVFAMLRGPKSIRTLFTTMRLTA
                     LRHSPLGFGLLISHPDPVLTDSWLEPCRADERICRDLATLLRHVAQTDLTDVATRLPR
                     FTKPVTLVWGQADRCFTPSLGRRLAQQFPNSTMIDVRGAKTFVALDDPGAVVGAIEKI
                     GAQRPA"
     gene            2207..2884
                     /locus_tag="VF874_20425"
     CDS             2207..2884
                     /locus_tag="VF874_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9178335.1"
                     /translation="MKVKSGDTPPARRTQAERTAATRARLVNSARKLFADKGFSAVST
                     EAIATAAGVTRGALYHQFEDKAALFTAVYEEVERELVADVAGRIVAIGPGDQLQAMRI
                     GAQLFLDLCSAPAVQQIVLIDAPAVLGWEKWRAVGVKYGLGVIETMLGQAIADGVIPE
                     QPVRPTAHVLLGALDEAALYISRATDAEDARTQMYAVCDRVIAGIAARDVEALPAPGL
                     KRSRRRP"
     gene            complement(2859..3314)
                     /locus_tag="VF874_20430"
     CDS             complement(2859..3314)
                     /locus_tag="VF874_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9178336.1"
                     /translation="MDGSVTVHMAAPAMQIWELVADVRNIGRFSPETFEAEWVGGATG
                     PAVGARFRGHVRRNEIGPVYWTTCRVTDCEPGREFGFDVLAGDRALNHWHYRLTPSGD
                     GTDVTESFRLADAAALRMYAALLGPLRRRRNVRDMRKTLERIKAVVETA"
     gene            3417..3923
                     /locus_tag="VF874_20435"
     CDS             3417..3923
                     /locus_tag="VF874_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178337.1"
                     /translation="MLLLVALTMVVAAGAAFLVLRTRGDALDRPQDPLSDEQSKAQVV
                     EPAKEIVALAKLHSAVGGYILMSCKNRTDPPYQGAIYLTFDVPKATDYFDQVAASLVS
                     HGWRQSPPPSRYLSGTTVSKNGVTAILYRDPDRTGSGIMKMYGECRNMVDHRNDTTDW
                     VDITDQFR"
     gene            4000..4527
                     /locus_tag="VF874_20440"
     CDS             4000..4527
                     /locus_tag="VF874_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178338.1"
                     /translation="MTDVRYARRLRRGPSVRVSLTTGFVGTIIIVLIGIAGPYLERAT
                     TTQQPVADPGFVAARSAPTAPADSAPPPPAIDERGFVNSFARCDGPQSVVALGRTEGS
                     EVAICAAADGQYVYKGVRLSRGTTLGLGGVTLTPNGFEARNDGVVYNVSPTELVISDG
                     STVLSREPMVAYQNR"
     gene            complement(4567..6714)
                     /locus_tag="VF874_20445"
     CDS             complement(4567..6714)
                     /locus_tag="VF874_20445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX9178339.1"
                     /translation="MNIVAPTASPRRGTPPRAGRRRDFRADIEGLRAVAVLAVVLFHA
                     GFPGVTGGFVGVDVFFVISGFLITGLLWRDVSTRGSIRLLRFYGGRARRLLPASATVL
                     VATAVVSCVLLPPLQARGVLGDAIACAVYVGNYRFAIDGTDYLASDAPPSPLQHYWSL
                     GVEEQFYLLWPALIIGTAWLVLRARRGARSSAAPYLLILGIVAAASFLISLASTHSFP
                     SWGFFSLPTRAWELAVGGLVALTASRWRRLSDRSAAIAGWGGLLLIVLTCIELNETTP
                     YPGTAALLPVFGTALVVGAGCAVSDRGVGRALSRPVMRTIGRVSYSWYLWHWPVLLLA
                     PALLGHSLGLAGRLVAVGVSFVLAVLTLGLIENPIRFAAPVRRSAARSLVLGAAVTAV
                     AACAATVLLVIRPLPVGQGTAAAAMTVTSPAPSSTQDGAVAQMTARVQAAVTASVAVQ
                     AVPSNLSPSLAEAPGDKPTVFLNGCVRSWREVGQDECASGDTASATTVALVGDSHAAM
                     WNPAFEPLAQQRHWRLETLGKVTCPLMELPITSPYLGRQYTECEQWRTDVLNRLAAER
                     PRLIVVSMSRRYGADFGFTSFDPAWVNGLSRLVSRLRATGAAVLVLGPIPDPLTTVPT
                     CVSGHLNDAQACSPPRSEAVNDAGNATEAAATTAAGGQYADLTSLFCTTDRCPVIVGN
                     NLVYRDDNHVTIEYAQALGPVIGAIADSALNSR"
     gene            complement(6775..7608)
                     /locus_tag="VF874_20450"
     CDS             complement(6775..7608)
                     /locus_tag="VF874_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9178340.1"
                     /translation="MIARVVQVTRRAVLQAGGLAAGTYVLANLLRPAVSRGEPPPAAA
                     AAPTYLAGSFLSAARGNVETNWAIARPPGQTQPLHPVIALHGRGGDAASVMDLGVADG
                     LARLTSAGVPPFAVVSVDGGDTYWHRRASGADSGAMVLMELLPMLAGQGLDTSRVGFL
                     GWSMGGYGALLLGARLGPARTAGICAVSPALWTSYMATDDGAFDSYNDWVKNTVFGLP
                     ALMSIPIRVDCGTADRFYAASRQFVAQLRRPVSGSFSPGGHDAAFWRGQLPDELRWMA
                     S"
     gene            7675..>8226
                     /locus_tag="VF874_20455"
     CDS             7675..>8226
                     /locus_tag="VF874_20455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9178341.1"
                     /translation="MTEWSGEDYANISALQRTMAEEAVASLRFAGNERVLDAGCGDGY
                     ITRMVAGMVPDGYVVGIDPARGMLAEAARGRSAASGPSFVRADVRQLPFDECFDAAVS
                     FNALHWVPQQHQALDQIASVVHSGAQVLIQVVCASERPSVEATAMTLCDRPRWAPHFV
                     GFNAPFIHVDPARFGELAAAAGLL"
BASE COUNT         1483 a         2695 c         2766 g         1282 t
ORIGIN      
        1 tggagaacct agagctgctg cagggcgaca ttgcgcagat ggacctggcg accctggggc
       61 agttcgacta catcgtctgc cacggcgtgt acagctgggt gccggacaat gtgcaggacg
      121 cgatcctggc cgccttccgc acgttgctgg ttcccgatgg cgtggcctac atcagctaca
      181 acgtgtatcc cggctggaaa gcgaaagaga tcgtgcgcga tgcgatgctg atgcgcagcg
      241 gtggccgcga aaccccaggg gagagggtgg cctatgcgcg cggcatgatc acgttcctgg
      301 aggacgtggc ccccgccgac agcgtgctgg ccagggcgct ggccgactac aaggtcatcg
      361 ccgccagttc aaaggattac tacctgctcc acgaggaact ggagacgttc aattcgccgt
      421 gctatttcct cgacaccgtc tggcctatct ggcggaggcg ctaacctcaa tcatgttccc
      481 gatgaattac ggcgacaagg ttgcggagcc gttgctcaag gaatgcgggc acagccaggt
      541 cctgatggaa cagtacctgg atttcgtcgt caaccgcccg tttcgacaaa cgcttctggt
      601 gcatgccgaa cgcgcacagc agatcagata ccagctggac cggagtcgtt acgaacgcct
      661 gcagttcggc gcctggcagc cagctgtcgg cggcgaaacc cgcctagacg actcgagcca
      721 ggaatatggt gatccggccg cggcgacggt ggtcacgcag aacccggccg tcaaggcggc
      781 gcttgatgcg ctcactgccc gctggccgtc gacgcagtca cggcaggaac tgctgaacga
      841 ggtgcagacg cagcttcgag ccgccggtgc cgacgccacc aacgtggagg ggtccatcga
      901 cgacctgctg gacttcttga ttgtgcgcgg acaggcgcgg taccgcctcg atccggtgat
      961 ggtggagcct gtgtccaccc cgctgcggct ggacgagccg atcaggcaga tggcgcagct
     1021 gacgcgcgca gacgccgacg cgtccacctt caacgtctgg cacgagacgg tggcgctgtc
     1081 gccggtggac agccatgtgc tgccactgct ggacggcacg cgtgaccgcg atgctctgga
     1141 ggatgcgctg ctggccctcg cccgcgaggg ccagctctcg ttcgagcgcg acggtcagcc
     1201 cgtgtcgggg gagtcggagc tgcgggaggc ggtggttgcg tacatcgacg cgcttccgca
     1261 gcgcctgacc gaaatgaagc tggtgtccgc gcgtcaggca ggtctctgag cgccgatctt
     1321 ctcgatcgcg ccaacgaccg ccccggggtc gtccagcgcc acaaaggttt tggcgccccg
     1381 cacgtcgatc atcgtggagt tcgggaactg ctgggccagc cgccggccca gcgacggcgt
     1441 gaagcagcgg tcggcctggc cccacacgag ggtgaccggc ttggtgaacc gcggaaggcg
     1501 agtcgcgacg tcggtcaagt cggtctgtgc gacgtgccgc agcagcgtcg cgaggtcgcg
     1561 gcagatgcgc tcgtccgcgc ggcacggctc cagccaagaa tccgtcagca ccggatccgg
     1621 atggctgatg agtagcccaa atccaagtgg agagtgacgc agggcggtca gccgcatcgt
     1681 cgtaaacagc gtcctgatcg acttcggccc gcgcagcatc gcgaatacgg cgtcgaacgg
     1741 gaacggcggg aacgtgtcga acgcgtcgca gttggtgagc accaagcggc cgacgtgctc
     1801 gggatgggcg tcgatcacca gctggcacaa gccgccgccg gtgtcgttgc ccaccagcgt
     1861 cacgtcggaa aggtcgagcg cgacgatgaa gtcgcgaatc atcgtcgcga ccccacgcgg
     1921 cgagaggtcg gctccggcgt tgaccggtat ccgatgggag ccgagcggcc aatccggcag
     1981 aatgcaacga aagcccttgc ccgccaactg ctctgcgact tcgtgccata gccggtggtc
     2041 caccagaatc ccgtggacga acaggaccgg cgggtgaggg gaatcgtcgg ggcccaacac
     2101 gtggtagtcg atggtcgctt gcttcaacgt cacctgcggc atgtccgcta tcctctccat
     2161 gtctgcaact tgcaaacagt tggtatgaaa gttacgtgca acctgtatga aagtcaagag
     2221 tggcgatacc ccgccggcgc gccgcacaca ggccgagcgg accgcggcga cccgggcgcg
     2281 gttggtcaac agtgcccgaa agttgttcgc cgacaagggt ttcagcgcgg tatcgacgga
     2341 agcgatcgcg acggcggccg gtgtgacgcg cggcgcgctt tatcaccagt tcgaggacaa
     2401 agccgcactg ttcaccgcgg tgtacgagga ggtggagcgc gagctggtcg ccgacgtggc
     2461 cgggcgcatc gtggccatcg ggccgggcga tcagttgcag gcgatgcgga tcggcgcgca
     2521 gctgttcttg gacctctgtt ctgcgccagc ggttcagcag atcgtgttga tcgacgcgcc
     2581 ggcggtgctg ggctgggaga agtggcgggc ggtgggcgtc aagtacgggt tgggcgtcat
     2641 cgagacgatg ctcggccaag ccatcgccga cggcgtgatt cccgaacaac ccgtgcgtcc
     2701 aacggctcac gtgctgctgg gcgcgctgga cgaggccgcg ctgtacatct cgcgcgccac
     2761 cgacgccgag gacgcccgca cgcagatgta cgcggtgtgc gaccgggtga tcgcgggcat
     2821 cgcggcgcgt gatgtcgaag cgctacccgc gcccgggctc aagcggtctc gacgacggcc
     2881 ttgatccgtt ccagtgtctt gcgcatgtcg cggacattgc ggcgtcgacg caacgggccg
     2941 agcaacgctg catacatgcg cagggcggca gcatcggcca gccggaagga ctccgtcacg
     3001 tcggtgccgt caccgctcgg cgtgagccgg tagtgccagt ggttgagcgc ccggtcgcca
     3061 gccagcacgt cgaacccgaa ctctcggccg ggttcgcagt ccgtgacccg gcatgtcgtc
     3121 cagtagacgg gcccgatttc gttgcgccgc acgtgtcccc ggaaccgcgc accgactgcc
     3181 gggccggtgg cgccgccgac ccattcagcc tcgaaggttt ccggcgaaaa ccgaccgatg
     3241 ttgcgaacgt cggccacgag ttcccagatc tgcattgccg gcgcggccat gtgcaccgtc
     3301 actgaaccgt ccatgctgcg attcaagcag aacgggaccg agagttacta ggctggttca
     3361 cagtggtgac agtcgacagc aagccaacta gacgggccgg cccgtcgcga ttactgatgc
     3421 tgttgctcgt tgcgctcacg atggtggtgg cggcaggcgc agctttccta gtgctacgaa
     3481 cgcgaggcga cgcactcgac cgcccgcaag atccgctgag tgacgaacag agcaaggccc
     3541 aggtcgtcga accggcgaag gagatcgtcg ccctcgccaa actccacagc gctgtcgggg
     3601 gttacatcct gatgtcgtgc aagaaccgga ccgacccgcc gtaccaaggt gcgatctacc
     3661 tgacctttga cgtgccgaag gcgaccgatt acttcgatca ggttgccgca tcgttggttt
     3721 cgcacggctg gcggcaatcg ccaccgccaa gtcggtatct ttccggcacc acggtctcca
     3781 agaacggtgt cactgccatc ctgtaccgcg accccgatcg cactggctcc gggatcatga
     3841 agatgtacgg cgaatgccgg aacatggtcg accatcgcaa tgacaccacc gactgggtcg
     3901 atatcaccga tcagttccgt taacacagac gcgactcgcc agcagcacaa tggccgcata
     3961 tctcacggat tccgggcagt aagtggtttc ctggagtttg tgactgacgt gaggtacgcc
     4021 cggcggctgc gccgcggtcc gtcggttcgc gtctcgttaa ccacgggctt tgtcggcacg
     4081 atcatcatcg tgctgatcgg tatcgcggga ccatatcttg agcgggcgac gacaactcaa
     4141 cagccggtgg ctgatccagg tttcgtcgct gcgcgatcgg cgccgactgc tccagccgat
     4201 tcggctccgc cgccgcccgc gatcgacgag cgcggcttcg tcaactcgtt cgcccggtgc
     4261 gacggtccgc agtccgtcgt agcactgggc cgcaccgaag ggtccgaggt ggccatctgc
     4321 gcggccgccg acgggcagta tgtgtacaag ggtgtccggt tgagccgcgg aaccacgctc
     4381 ggcctgggcg gtgtgacgtt gactcccaat ggattcgagg cacgaaacga cggggtcgtc
     4441 tataacgtgt cgcccaccga gctcgtgatc tccgacggca gcaccgtcct gtcccgcgag
     4501 ccgatggtcg cgtaccagaa tcggtgatga cgactcgacg tggtcgttcc tgtaccgcgc
     4561 taacactcag cgagagttga gtgcgctgtc cgcgatcgca ccgattacgg gtccgagcgc
     4621 ctgagcgtac tcgatcgtga cgtggttgtc gtcgcggtag acgaggttgt tgccgacgat
     4681 caccgggcag cgatcggtgg tgcagaacag ggacgtgagg tctgcgtatt gcccgccggc
     4741 cgccgtggtc gccgcggcct cagtcgcatt gccggcgtcg ttcaccgcct ccgagcgcgg
     4801 cggcgaacaa gcctgcgcgt cgttcagatg gccggataca caggtcggca cggtggtgag
     4861 cgggtccggg atcgggccga gcaccaaaac ggctgccccc gttgcccgca accggctcac
     4921 caaccggctc aggccgttca cccaggccgg gtcaaacgac gtgaacccga agtccgcgcc
     4981 gtaccggcgc gacatgctga ccacgatcag gcgcggtcgc tcggcagcca acctgttcaa
     5041 cacgtcggtt cgccactgct cgcattcggt gtactgccgg ccaagatagg ggctggtgat
     5101 cggcagctcc atcaacggac aggtgacctt gccgagtgtc tctagccgcc aatgccgttg
     5161 ctgggcaaga ggttcgaacg ccggattcca catcgccgca tgcgagtcgc cgaccaacgc
     5221 caccgtggtg gccgacgcgg tgtcgcccga ggcgcactcg tcttggccga cttcacgcca
     5281 cgaccgcaca cacccgttga ggaaaaccgt tggcttgtcg cccggcgcct cggcaagtga
     5341 gggggacagg ttcgatggaa cggcttgcac cgcaaccgat gccgtcacgg cggcctgaac
     5401 tcgagcggtc atctgcgcaa ccgcgccgtc ctgcgtgctg gacggcgcgg gcgacgtgac
     5461 cgtcatcgcc gcggccgctg ttccttgccc gaccggaagc ggccgaatca ccaacagcac
     5521 cgtggccgca cacgcggcga ccgcggtgac ggccgcgccg agcaccaggc tgcgcgccgc
     5581 agaccgcctg acgggtgccg cgaatcggat cggattttcg atgaggccga gcgtcaacac
     5641 cgcgagtacg aacgacaccc ctaccgcaac gaggcgaccc gcgagtccga gcgagtgtcc
     5701 cagaagtgcc ggcgcaagca gcagcacagg ccagtgccac agataccagg agtacgacac
     5761 ccgaccgatc gtgcgcatta ccggtcgcga cagcgcacgg ccgacgcccc tgtcggacac
     5821 ggcacacccg gcccccacca ccaacgcggt cccgaagacg ggcagcagcg cggcggtacc
     5881 gggatatggt gtcgtctcgt tcaactcgat gcaggtgagg acgatcagaa gcagaccgcc
     5941 ccagcccgcg atcgctgcgc tgcggtcgga cagccggcgc catcggctgg cggtgagcgc
     6001 aaccaatccg ccgacggcca gctcccaggc gcgggtcggc aacgagaaga acccccatga
     6061 cggaaacgag tgcgtcgacg ccagcgagat cagaaacgaa gccgccgcga cgattccgag
     6121 gatcaggagg tacggcgcgg cgctggagcg agccccgcgg cgggctcgga gcaccagcca
     6181 cgcggtgccg atgatcagtg cgggccacag cagatagaac tgctcctcga cacccagcga
     6241 ccagtagtgc tgcaacggcg atgggggagc gtcgcttgcg agatagtccg tgccgtcgat
     6301 cgcgaatcgg tagttgccga cgtagaccgc gcacgcgatc gcgtcgccga ggacgccgcg
     6361 ggcctgcaac ggcggcagca gcacacacga cacgaccgcc gtggcgacga gcaccgttgc
     6421 cgaggcgggc agcaggcggc gggcccgccc gccgtagaac cggagcagac gaatacttcc
     6481 gcgcgtgctg acgtcgcgcc acaggagccc ggtaatcaag aacccggaga tcacgaagaa
     6541 gacgtccaca cccacgaaac cacctgtgac acctgggaat ccggcgtgga agagcaccac
     6601 ggcgagcacc gcgacggcgc gcagcccctc gatgtccgcg cggaagtcac gccgcctacc
     6661 ggcgcgggga ggcgtgccgc gtcggggtga agcggtgggc gcaacgatgt tcactcgagc
     6721 agcgatcttg ccacgcccag gccgaagaac ccgggaggtc agcgggcacg tcgcctacga
     6781 cgccatccac ctcagttcgt cgggcagttg accgcgccag aacgcggcat cgtgtccgcc
     6841 cggggagaag ctgccggaga cgggccttcg cagctgcgca acgaattgcc ggctggcggc
     6901 gtagaaccgg tcagcggtgc cgcagtccac ccggatcggg atcgacatca gcgcgggcag
     6961 accgaacacg gtgttcttca cccagtcgtt gtagctgtcg aaggcgccgt cgtcggtcgc
     7021 catgtacgac gtccacagtg ccgggctcac cgcgcagata ccggcggttc gcgcggggcc
     7081 cagccgggcg cccagtagca gggctccgta gccgcccatc gaccagccga gaaacccgac
     7141 acgtgacgtg tcgagcccct gcccggcaag catcggcaag agttccatga gcaccatcgc
     7201 tccggagtcc gcacctgagg cccgcctgtg ccagtaggtg tcgccgccgt ccacgctgac
     7261 gacggcgaac ggcggcacac cggcgctggt gagccgggcc agtccgtcag caacaccgag
     7321 atccatcaca ctggccgcgt cgccgccgcg cccgtgcagt gcaatgacgg gatgcaacgg
     7381 ctgagtttgt cccggtggac gggcgatggc ccagttcgtc tcgacgtttc cgcgagccgc
     7441 ggagaggaac gagccggcga ggtaggtcgg cgccgcagcc gccgccggcg gtggttcgcc
     7501 gcgagaaacc gctggccgca acaggtttgc cagtacgtaa gtacccgccg ccaagccgcc
     7561 ggcctgcagg accgctcgac gtgtcacctg caccacacgg gcaatcatcg cagtgagcca
     7621 gcgcgctcat tgaaacgcgt tcgcatcaaa gcaattacag tacggtagag ccgtatgacc
     7681 gagtggagcg gcgaggacta cgccaacatc agtgccctgc agcgcacgat ggccgaagag
     7741 gccgtcgcct cgcttcgatt cgccggcaac gaacgcgtcc ttgacgcggg atgcggcgac
     7801 ggatacatca cgcggatggt ggccggcatg gtgcccgacg ggtacgtcgt cggaatcgac
     7861 ccggcccgcg gcatgctcgc cgaggcggcg cgcggcaggt cggctgcttc cgggccgtcg
     7921 ttcgtgcggg ccgatgtccg gcagttgccc ttcgacgaat gtttcgacgc cgcagtgtcg
     7981 ttcaatgcgc ttcattgggt gccgcagcag caccaggcgc tggaccagat cgcctcggtc
     8041 gtccactcgg gcgcccaggt actgatccaa gtcgtgtgtg catccgagcg gccgagcgtg
     8101 gaagcgaccg ccatgacact gtgtgaccgt cctcggtggg cacctcactt cgtcggcttc
     8161 aacgcgccgt tcatccatgt ggacccggcg cgctttggcg agttggccgc ggcggcaggg
     8221 ctgttg
//
LOCUS       DASWBF010000546        10451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_844585,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000546 DASWBF010000000
VERSION     DASWBF010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844585"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VF874_20460"
     CDS             <1..307
                     /locus_tag="VF874_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178342.1"
                     /translation="DELLSRWRQADERINSYIAVGNYQAATQVALGTGEQDSTPAFDR
                     LEQALNNGIEESRRQLRNDIVDARAVLSGATVGAVLLSVVAALAVTVGLWPRMSEYR"
     gene            304..1263
                     /locus_tag="VF874_20465"
     CDS             304..1263
                     /locus_tag="VF874_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9178343.1"
                     /translation="MKRALAFLAAVVVLVAGCGQSDTLTAQPSLTLAPPTPANMQELP
                     PQQNPPSDPNGTCNRTESLRPFPDPKDAEAAVAAIRQRGRLLVGLDIGSNLFSFRDPI
                     TGEITGFDVDIAGEVARDIFGTPSQVEYRILSSADRIKALQNNEVDIVVKTMTITCER
                     RKQVAFSNVYLLANQRILAPRDSAISQASDLSGKRVCAARGTTSINRLEKISPAPIIV
                     SVVTWADCLVALQQRQVDAVSTDDSILAGLVSQDPYLHITGPDLEEEPYGIGVNLGNT
                     GLVRFVNGTLERIRRDGTWYALYRKWLTVLGPTPAPPTPRYSD"
     gene            1263..3509
                     /locus_tag="VF874_20470"
     CDS             1263..3509
                     /locus_tag="VF874_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase PknG"
                     /protein_id="HEX9178344.1"
                     /translation="MGQPEEQEIVGTQPAAFGIDYAEDSSSILRPMATQAIFRPEIEV
                     GSEGVDTDASVDEHTTTVTRQLSLIRRLGGGLVEIPRVPEKDPLAALMTNPVVAESKR
                     FCWNCGRPVGRSTPKGEAKSEGYCPHCGSQYSFLPQLGPGDKIAGQYEIKGCIAHGGL
                     GWVYLAVDHNVNERPVVLKGLVHAGDAEAQAIAMAERQFLAEVTHPSIVKIFNFVQHP
                     DKHGEPVGYIVMEYVGGTSLKPAKGHKLPVAQAIAYMMEILPALGYLHSIGLAYNDLK
                     PENIMITEEQLKLIDLGAVSRLASYGYLYGTPGYQAPEIVRTGPTVATDIYSVGRTLA
                     ALTLNLRTHKGRYAEGLPEDDPVLATYDSYARLLRRATDPDPARRFASAEEMWAQLLG
                     VLREVVAHDSGAPRPGLSTVFSPSRSTFAVELVVAHTDVYLDGLVHAEKLTASEIVTA
                     LPVPLVDPSDVGAAVLSATVLSQPVQTLDSLRAARHGTLDSEGVDLTESVELPLMEVR
                     ALLDLGDVAKAVRKLDALAERVGWRWRMVWFRAVAALLTGDHDAATKHFTEVLDTQPG
                     ELAPKLALAATAELAGNPDGLSFYDTVWSTDNGVVSAGFGLARARSTQGDRDGAVRTL
                     DAVPATSRHFTTARLTSAVTLLSGRSMSELTEEQIRDAARRVEALPDTEPRVLQIRAL
                     VLGTALDWIGENTASTNHILGFPFTPHGLRLGVEASLRGLARVAPTRGHRYALVDTAN
                     AVRPSSTF"
     gene            complement(3535..4179)
                     /locus_tag="VF874_20475"
                     /pseudo
     CDS             complement(3535..4179)
                     /locus_tag="VF874_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and ANTAR domain-containing protein"
     gene            complement(4327..5445)
                     /locus_tag="VF874_20480"
     CDS             complement(4327..5445)
                     /locus_tag="VF874_20480"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX9178345.1"
                     /translation="MIATVLVLNSGSSTLKYAVVDPASAAPVAHGVVERIGEDEVADH
                     EAALRIVFGQLTEPEGLVAVGHRIVHGGNAFYEPTVITDAVVEKLEQLSALAPLHNPP
                     GVLSVQVARAVLPALPHVAVFDTAFFQHLPAAAATYAIDHEVSVRWDVRRYGFHGTSH
                     RYVSEQAADFLGQPLESLSQIVLHLGNGASAAAIVGGRPVDTSMGLTPMEGLVMGTRA
                     GDIDPGVVFYLWRTAQMDVEQIEAMLNRHSGMVGLCGEIDFRRVHQLIESGDARAQLA
                     YDIYIHRLRKYVGAYLALLGGTDVITFTAGVGEHDPAVRRDALSGLAALGIELDEDLN
                     ISGARGPRRISFEQSPTTVLVIPTNEELAIARACLTVI"
     gene            complement(5442..7577)
                     /gene="pta"
                     /locus_tag="VF874_20485"
     CDS             complement(5442..7577)
                     /gene="pta"
                     /locus_tag="VF874_20485"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX9178346.1"
                     /translation="MSSASSSDALGASGSSSIYIASPESDTGKSTIALGVLHRLAVTV
                     AKVGVFRPITRRGEDRDYILDLLLAYATAGLPYEQCVGVTYQQLHEDEDAAIAEIVDR
                     YHDVADQCDAVVIVGSDYTDVASPTELSVNARIAVNLGAPVLLSVRAKGRTTDQVAQV
                     VSLCLDKLASQHAHTAAVVANRCDPPEMTAMAAALLSALRASGSSPQRDEPKVYVLPE
                     EPLLVAPTVADLQRSVNGTLVSGSEALMSREVMSVLVAGMTSEHVLERLADGAAVITP
                     GDRSDVVLTVSSAHAAEGFPSLSTVILNGGLALHPSIAALVNGLGLRLPMIATELDSF
                     QAASAIATTRGRMTATSQRKIDIALSLMDSCVDMSELLAQLAVPIPTVTTPQMFTYQL
                     TEWARADRKHIVLPEGEDDRILQAAGRLLQRGVSDLTILGTEAHIRSHAAELNVDLSS
                     ATLLDPLTSELSEKFVEQYIELRRHKGATIEKARETVIDVSYFGTFLVYNGIVDGMVS
                     GATHTTAHTVRPAFEIIKTKPDVSTVSSIFLMCLSDRVLAYGDCAIVPDPTSEQLADI
                     AISSARTAAQFGIEPRVAMLSYSTGTSGSGAEVEKVRRATELVREREPELLVDGPIQY
                     DAAVEPSVAATKAPDSPVAGRATVLIFPDLNTGNNTYKAVQRSAGAIAIGPVLQGLKK
                     PVNDLSRGALVDDIVNTVAITAIQVQENT"
     gene            complement(7579..8589)
                     /gene="fgd"
                     /locus_tag="VF874_20490"
     CDS             complement(7579..8589)
                     /gene="fgd"
                     /locus_tag="VF874_20490"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HEX9178347.1"
                     /translation="MAELKLGYKASAEQFAPRELVELGVLAEQHGMDSATVSDHFQPW
                     RHKGGHAPFSLGWMTAVGERTERIALGTSVLTPTFRYNPAVTAQAFATMGCLYPGRIF
                     LGVGTGEALNEIATGYIGQWPDFKERFARLRESVRLMRELWAGDRVSFDGDYYHTRGA
                     SIYDVPEGGIPIYVAAGGPAVAKYAGRAGDGFICTSGKGEELYTEKLIPAVKEGAAAA
                     NRNVDDIDKMIEIKISYDADPELALENTRFWAPLSLTAEQKHSIDDPIEMEKAADELP
                     IEQVAKRWIVASDPDEAVEKVRAYLDYGLNHLVFHAPGHDQRRFLALFEKDLAPRLRR
                     LG"
     gene            8625..9356
                     /locus_tag="VF874_20495"
     CDS             8625..9356
                     /locus_tag="VF874_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9178348.1"
                     /translation="MAWRLASVTDSLHFVQGDLVNWAVATGSDGVTLFDAGLPGDRDD
                     VLASLAELGFGADDVRAIVLTHAHVDHLGSAVWFAKTHGTPVFCHADEVGHAKREYLE
                     QASPLDVAANIWRPGWAPWAVRLLRKGGMVRDGIPTAQALTAEIATTLPGQPMAIPTP
                     GHTGGHCSFIVDGVLVSGDALVTGHAASRRTGPQLLPSVFNHDNAGCRRSLGALALLD
                     TDVLLPGHGPVWRGPIREAIQQVLA"
     gene            9353..10192
                     /locus_tag="VF874_20500"
     CDS             9353..10192
                     /locus_tag="VF874_20500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9178349.1"
                     /translation="MTDFLRDALRNIWSTAAPAWERHADYADDRGAVITQAMLEAAEL
                     RPEARVLELACGPGGVGLAAAEIVGPDGEVVCSDVAPEMTEIAAQRAAARNLRNVRTA
                     QIGMEDIPLPDASFDAVLCREGLMLVIDPAAAVREAHRVLVPGGRAVYAVWGPRDRNP
                     WLGVLLDAITAHLGIPVPPPGMPDPFALSDYGRLEELLGEAGFREVSIREVATPMNAS
                     SFDEWWAVVPSLAGPVGPLLEAQPPEVSAAIRGEASAILDEFRTDSGYDLPGLSFVGV
                     GHH"
     gene            complement(10154..>10451)
                     /locus_tag="VF874_20505"
     CDS             complement(10154..>10451)
                     /locus_tag="VF874_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178350.1"
                     /translation="VEHGDVLGRVHRRELPPRQQFDGTPGEVAADIDHYVQSDACDGF
                     IFVPHLTPHGLDEFVDKVVPLLQERGTYRTEYSGRTLRDHLGLVVADADETQAR"
BASE COUNT         1669 a         3489 c         3577 g         1716 t
ORIGIN      
        1 cgacgagctg ctgtcgcgat ggcggcaggc cgatgagcgg atcaactctt acatcgcggt
       61 cggcaactac caggccgcga cgcaggtggc gctgggcacc ggcgagcagg attcgacgcc
      121 ggcgttcgat cgactggagc aggcgctcaa caacggcatc gaggagagcc gccgtcaact
      181 gcgcaacgac atcgtcgatg cacgggcggt gctgtcgggc gcgacggtcg gcgcggtcct
      241 cctgtcggtg gtagccgcgc tggcggtgac ggtgggattg tggccacgga tgagcgagta
      301 cagatgaaaa gggcattggc tttcttggcc gcggttgtcg tcctggtggc cggctgcggg
      361 cagtcggata cgttgaccgc tcagccgtcg ctgacgctgg cgccgccgac gccggcgaac
      421 atgcaggagc tgccgccgca gcagaatccc cccagcgatc cgaacggaac ttgcaaccgg
      481 accgagagcc tgcggccgtt ccccgacccg aaggacgccg aggcagccgt cgccgccatc
      541 cggcagcgcg ggcggcttct ggtcggactg gacatcggca gcaacctgtt cagcttccgc
      601 gacccgatca ccggagagat caccggtttc gacgtcgaca tcgcagggga ggtggcacgc
      661 gacatcttcg gcacgccgtc acaagtcgag taccggatcc tgtcgtcggc cgaccggatc
      721 aaggccttgc agaacaacga ggtggacatc gtcgtcaaga cgatgaccat cacctgcgag
      781 cggcgcaagc aggtggcgtt ctccaacgtg tacctgctgg cgaatcagcg gattctggcc
      841 ccgcgggatt ctgcgatcag ccaagcctcg gatttgtccg gcaagcgggt gtgcgcggcc
      901 cgcggcacca cgtcgatcaa ccggctggaa aagatcagcc cggcgccgat catcgtgtcg
      961 gtggtgacgt gggccgattg cctggtcgca ctgcaacagc ggcaggtcga cgcggtcagc
     1021 accgacgatt cgatcctcgc cgggctggtg tcgcaagacc cgtacctgca tatcaccgga
     1081 ccggatctgg aggaggagcc gtacggcatc ggtgtcaatc tgggtaacac cgggctggtg
     1141 cggttcgtca acggcacgct ggagcggatc cgccgcgacg gaacgtggta tgcgctgtac
     1201 cgcaagtggc tgaccgtgtt gggcccgacg ccggcgccac cgacgccgag gtattcggac
     1261 tgatgggcca acccgaggag caagaaatcg tcggcaccca gccggctgcc ttcgggatcg
     1321 actacgccga ggactcgtcg tcgatactgc gtccgatggc gacgcaggcg atcttccgcc
     1381 ccgagatcga ggtgggctcg gagggcgtgg acaccgacgc cagcgtcgac gagcacacca
     1441 ccacagtcac gcgccagctg tccctcatcc ggcggctcgg cggcgggctc gtcgagatcc
     1501 cacgcgtacc cgagaaggat ccgctcgcgg cgttgatgac gaatcccgtt gtcgcagagt
     1561 cgaagcgatt ctgctggaat tgcgggcggc cggtaggacg ctcgacgccg aagggtgagg
     1621 ccaagagcga gggctattgc ccgcactgcg gcagccagta ttcgttcctg ccgcagctcg
     1681 ggccgggcga caagatcgcc gggcagtacg agatcaaggg ctgcatcgcg cacggcggcc
     1741 tgggctgggt gtacctggcc gtcgaccaca acgtcaacga gcggcccgtg gtgctcaaag
     1801 gcctggtgca cgccggcgat gccgaggcgc aggcgatcgc gatggccgag cggcagttcc
     1861 tggcggaggt tacgcatccg tcgatcgtga agatcttcaa cttcgtccag caccccgaca
     1921 agcacgggga gccggtcggc tacatcgtga tggagtacgt cggcggcacg tcgctgaaac
     1981 cggcgaaggg tcacaagctg ccggtcgctc aggccatcgc ctacatgatg gagatcctgc
     2041 ccgcgctggg atacctgcac tcaatcggct tggcgtacaa cgacttgaag cccgagaaca
     2101 tcatgatcac cgaggagcag ctcaagctga tcgacctcgg cgcggtgtcg cggctcgcgt
     2161 cttacggcta cctctacggg acgccggggt atcaggcgcc cgagatcgtg cgaaccggcc
     2221 cgaccgtggc caccgacatc tactcggtgg gccgcaccct ggcggcactg acgctgaacc
     2281 tccgcaccca caagggccgg tacgccgagg ggctgccgga ggacgacccg gtgctggcta
     2341 cctacgactc gtacgcccgg ctgctgcgcc gggcgaccga cccggatcct gcgcggcggt
     2401 tcgccagcgc cgaggagatg tgggcacagc tgctcggtgt gctgcgggag gtggtcgcgc
     2461 acgacagcgg cgcgccgcgg ccgggattgt cgacggtgtt cagcccgtcg cggtcgacgt
     2521 tcgcggtgga actcgtcgtc gcccacaccg acgtatacct cgacggtctg gtgcacgcgg
     2581 agaagctgac ggcttccgag atcgtcaccg cgctgccggt gcccctggtg gatccgtccg
     2641 acgtcggggc cgccgtgctg tcggcgacgg tgctgtcgca gccggtgcag acgctggact
     2701 cgctgcgagc cgcccggcac ggcacgctcg acagcgaggg cgtcgacctc accgagtcgg
     2761 tggagctgcc gctgatggag gtacgcgcgc ttctggacct cggcgacgtc gccaaggccg
     2821 tccgcaagct cgacgcgctg gccgagcgcg tcggctggcg atggcgaatg gtgtggttcc
     2881 gcgcggttgc cgcgctgttg acgggtgacc acgacgctgc gacaaagcat ttcaccgagg
     2941 tgctggacac gcagcccggt gagctggccc cgaagctggc gttggcggcg accgccgaac
     3001 tcgccggaaa cccggacggg ctgagcttct acgacacggt gtggagcacc gacaacggcg
     3061 tggtttcggc cgggttcggg ctggcgcggg cgcggtcgac gcagggcgac cgggacggtg
     3121 cggtgcgcac gttggacgcg gtgccggcca cgtcgcgaca cttcaccacc gcgcggctga
     3181 cgagcgcggt gacgctgctg tcgggccggt cgatgagcga gctgaccgag gagcagatcc
     3241 gcgacgcggc ccggcgcgtc gaggccctgc cggataccga acccagggtg ctgcagatcc
     3301 gcgcgctggt gctgggcacg gcgctggact ggatcggcga gaacaccgcc agcaccaacc
     3361 acatcctcgg gttcccgttc acgccgcacg ggctgcggct cggcgtcgag gcgtcgttgc
     3421 gcggcctggc gcgggtggcg ccgacccgcg ggcaccggta cgcactggtc gacacggcca
     3481 acgcggtccg gccgtcgtca actttttagg ccgacgttca taagcgtcgt cctcgatccg
     3541 gtggtgggcc gtcagcagca ggttgtcgta gaccgatcgc ggcggcagcg cctcgtcgtg
     3601 cgcgacggcg gaaaagtcgg cggcgatctg ctcggccaag ggccgcagct tgacgttggt
     3661 ttcctgtgac cgccacttca gcaggtcgaa cgccgcctgc gcatcgatcc cgtagatcgc
     3721 catcagcatg cccttggcct gctcgatcgc cgcacggctc ttcgtgatct tggcgacctc
     3781 ggcggtgagg tgatcctggc gcgcctgttc ggccggggtg acgtcgacgt agaacccgtg
     3841 cgtgccgatg atcgagccgt cgccgtcgcg catctggtcg ccgacgacca ccacgtgatg
     3901 cacgctgcct tggacgtcgc ggatccggtg gcgactgctg aacgcctggc gggtctgccg
     3961 gatcagggcg agagtgtcgg cgatctggcg gtagtcgtcg ggatgtttgt gcgacagcac
     4021 caggtcggtg gttggaacga cggtgccggg ctcatagccg tgcatcttcg ccacctgcgg
     4081 cgaccattcc caccgctcgt cgtcgaagaa gtagcgaaac cagcccacgc gctcgggagc
     4141 gccccacgcg agggcctctc caccgtgttc actgatcatg tctggccgcc tcagcactgc
     4201 gctgtcggga ccgcaccttc cggcgcgttc ggccagggct gcgtccctcc cgtcaaccgt
     4261 gcaaatcact gcttccagtg tgccaacact tgcctcgaac gtgcggccac tgcgaaaaat
     4321 ccggcgctag atcaccgtga ggcatgcccg ggcgatcgcc agttcctcgt tggtcgggat
     4381 caccagcacc gtcgtcggcg actgctcgaa cgagatccgg cgcgggccgc gggcaccgct
     4441 gatgttgaga tcctcgtcga gctcgatgcc cagggctgcc agcccgctca gcgcgtccct
     4501 gcgtaccgcg gggtcgtgct cgccgacacc ggcagtgaac gtgatcacgt cggtgccgcc
     4561 cagcagcgcc aggtatgccc cgacgtactt gcgcagccgg tgaatgtaga tgtcatacgc
     4621 caattgtgct cgcgcatcac ccgattcgat cagctggtga acgcggcgga agtcgatctc
     4681 gccgcacaag ccgaccatgc cggagtgccg gttgagcatc gcctcgatct gctcgacgtc
     4741 catctgagcc gtgcgccaca ggtagaacac cacgccgggg tcgatgtcac cggcgcgggt
     4801 gcccatcacg aggccctcca tcggggtcag tcccatcgac gtgtccaccg gccggccccc
     4861 gacgatcgct gccgccgagg cgccgttgcc cagatgcagc acgatctggc tcagcgactc
     4921 gagcggctga cccaagaaat ctgccgcctg ctcgctgacg taccggtgcg acgtgccgtg
     4981 gaagccgtag cggcgcacgt cccagcgcac gctcacctcg tgatcgatcg cgtacgtggc
     5041 cgccgccgcg ggcaggtgtt ggaagaacgc ggtgtcgaac accgccacgt gcggcagtgc
     5101 cgggagcacg gcgcgcgcga cctggacgct caacacgccc ggcgggttgt gcagcggcgc
     5161 aagcgccgac agctgttcga gcttctcgac caccgcgtcg gtgatcacgg tcggctcgta
     5221 gaacgcgttt ccgccgtgca caattcgatg cccgactgcc accaggcctt cgggctcggt
     5281 gagttgcccg aacacgatgc gcagcgccgc ctcgtggtcg gcgacctcgt cctcgccgat
     5341 tcgctcgacg acgccgtggg ccaccggagc cgccgacgcg ggatcgacga ccgcgtactt
     5401 cagcgtcgaa gaaccggagt tcagcacgag caccgttgcg atcacgtgtt ctcctgcacc
     5461 tggatcgccg tgatcgcgac ggtgttgacg atgtcgtcga cgagcgcacc gcgggacagg
     5521 tcgttgacgg gcttcttcaa gccctgcagc accgggccga tcgcgatcgc gccggcgctg
     5581 cgctgcaccg ccttgtaggt gttgttgccg gtgttgaggt ccgggaagat cagcaccgtc
     5641 gcgcgaccgg cgacgggtga gtccggcgcc ttggtggccg ccaccgacgg ttccacggcc
     5701 gcgtcgtact ggatgggtcc gtcgaccagc agctccggct ccctttcgcg caccagttcg
     5761 gttgccctgc gcaccttttc gacctctgca cccgatcccg acgtgccagt ggagtacgac
     5821 agcatcgcga cgcgcggctc gatgccgaac tgtgccgccg tgcgtgccga cgagatcgcg
     5881 atgtcggcca gctgctccga cgtcggatcg gggacgatcg cgcagtcgcc gtacgccagc
     5941 acccgatcgg aaaggcacat caggaagatg ctcgacaccg tcgacacgtc gggcttcgtc
     6001 ttgatgatct cgaacgccgg ccgcacggtg tgagccgtgg tgtgggtggc ccccgacacc
     6061 atgccgtcga cgatgccgtt gtacacaaga aacgttccga agtacgacac gtcgatgacg
     6121 gtctcgcggg ccttctcgat cgtggcgccc ttgtgccggc gcagctcgat gtactgctcg
     6181 acgaacttct ccgacagctc actggtgagc gggtccagca aggtggccga cgacaggtct
     6241 acgttgagtt cggctgcgtg agaacggatg tgggcctctg tcccgaggat cgtcaagtcg
     6301 gatacgccgc gttgcaacaa ccggcccgcc gcttgcaaga tcctgtcgtc ctcgccttcg
     6361 ggcagcacga tgtgtttgcg gtcggcacgc gcccactccg tcagctggta ggtgaacatc
     6421 tgcggcgtcg tcaccgttgg aatcggaacc gccagctgcg caagcagttc cgacatgtcg
     6481 acgcagctgt ccatcagcga cagcgcgatg tcgatcttgc gctgcgacgt ggccgtcatg
     6541 cgcccccggg tcgtcgcgat cgcgctcgcc gcttgaaagg aatccagttc ggtcgcgatc
     6601 atcggcagcc gaaggcccag gccgttcacc agcgccgcga tcgacgggtg caaagccagc
     6661 ccgccgttga ggatcaccgt cgacaacgac ggaaaccctt cggctgcatg ggcactcgac
     6721 acggtcagca cgacgtcgga gcggtcgcct ggcgtgatca cggcggcccc gtccgccagc
     6781 cgctcgagca cgtgctcaga cgtcatgccc gccaccagca ccgacatcac ctcgcggctc
     6841 atcagcgcct cgctgccgct gaccagcgtc ccgttcaccg accgctgcag gtcggccact
     6901 gtcggggcga ccagcagcgg ctcctccggc agcacgtaca ccttcggctc gtctcgctgc
     6961 ggcgatgagc cgcttgcgcg aagagcagaa agcagcgccg cggccatcgc tgtcatctcc
     7021 ggcggatcgc accgattcgc cacgacggcg gcggtgtgcg catgctgact ggccagcttg
     7081 tcgaggcaca ggctcacgac ctgggccacc tggtcggtgg tacggccctt ggcccgcacc
     7141 gaaagcaaca ccggcgcacc gagattcacc gcgatccgcg cgttgacact caactcggtg
     7201 gggctggcga cgtcggtgta gtcgctgccg acgatcacca cagcgtcgca ctgatcggcg
     7261 acgtcgtggt agcggtcgac gatctcggcg atcgcggcgt cctcgtcctc gtgcagttgc
     7321 tgataggtga cgcccacgca ctgctcgtac ggcagtcccg cggtggcata ggccaacaac
     7381 aggtccagga tgtagtcgcg gtcctcaccc cggcgtgtga tcggccggaa cacaccgact
     7441 ttcgcgaccg taaccgccaa ccggtgcagc acgcccaaag cgatcgtcga cttgccggtg
     7501 tcgctctcgg gggacgcgat gtagatcgac gatgagccgc ttgcgccaag agcatcagag
     7561 gaggacgcgc tgctcacgtc agcccaaccg acgcagccgg ggggcgaggt ccttctcgaa
     7621 cagcgccagg aaccgccgct ggtcgtggcc cggcgcgtgg aacaccaggt ggttgagccc
     7681 gtaatcgaga taggcgcgca ccttttcgac cgcctcgtcg ggatcggacg cgacgatcca
     7741 ccgctttgcc acctgttcga tcggcagctc gtcggcggcc ttttccatct cgatcgggtc
     7801 gtcgatgctg tgtttctgct cggcggtcag cgacagcggc gcccagaacc gcgtgttctc
     7861 cagcgccagc tcgggatcgg cgtcgtagga gatcttgatc tcgatcatct tgtcgatgtc
     7921 gtcgacattg cgattcgcgg ccgcggcgcc ctctttgacg gccgggatca gcttctcggt
     7981 gtacagctct tcgcccttgc ctgacgtgca gatgaagccg tcgccggcgc ggcccgcgta
     8041 cttcgccacg gcgggaccgc ccgcggcgac gtagatcggg atgccgccct cgggcacgtc
     8101 gtaaatcgac gccccccggg tgtggtagta gtcgccgtcg aagctgaccc ggtcgccggc
     8161 ccacagctcg cgcatcaggc gcaccgactc gcgcaaccgg gcgaaccgct ccttgaagtc
     8221 cggccactgt ccgatgtatc cggtagcgat ctcgttgagc gcctcgccgg tgccgacgcc
     8281 gaggaagatg cggcccgggt acaggcagcc catcgttgcg aacgcctgcg cggtgaccgc
     8341 agggttgtag cggaaagtcg gggtgagcac cgacgtgccg agcgcgatcc gttcggttcg
     8401 ttcccccacc gcggtcatcc accccagtga gaacggggcg tgaccgccct tgtgacgcca
     8461 cggctggaag tggtcgctga cggtggcgct gtccatgccg tgctgttcgg ccagcactcc
     8521 cagctcgacg agctctcgcg gcgcgaattg ttccgccgac gctttgtatc ccagtttcag
     8581 ttcagccacg tgtcagttgt actccgtcgc tagactcggc gggcatggcg tggaggctcg
     8641 cttcggtcac cgacagtctt catttcgtgc aaggcgactt ggtgaactgg gcggtggcga
     8701 ctggcagcga cggtgtcacg ttgttcgacg ccgggctgcc gggcgatcgc gacgacgtgc
     8761 tggcgtcact ggccgagttg ggcttcggcg ccgacgacgt ccgcgcgatc gtgctgaccc
     8821 acgcccacgt cgaccatttg ggttcggcgg tatggttcgc caaaacccat ggcacaccgg
     8881 tgttttgcca tgccgacgag gtcgggcacg ccaagcgcga atacctggag caggcctctc
     8941 cgctggacgt cgccgcgaac atctggcggc cgggctgggc gccgtgggcc gtacggcttc
     9001 tgcgcaaggg cggcatggtg cgcgacggca tcccgaccgc gcaggcgttg accgcggaaa
     9061 tcgccacgac gctccccggc cagccgatgg ccattccgac gccggggcac accggcgggc
     9121 actgcagctt catcgtggac ggcgtgctgg tcagcggcga cgcgctggtc accggccacg
     9181 cggcgagccg ccgcaccgga ccgcagctgt tgccgtcggt gttcaaccac gacaacgcgg
     9241 gctgccgccg cagcctcggc gcgctggcgc tgctggacac cgacgtgctg ctgccgggac
     9301 acgggccggt gtggcgcggc ccgattcggg aggctattca gcaggtgctc gcgtgacgga
     9361 cttcttacgg gacgcgttgc gcaacatctg gtcgacggcg gcgcccgcct gggagcggca
     9421 cgccgactac gccgacgacc gcggcgcggt gatcacgcag gcgatgctcg aagcggccga
     9481 actgcggccc gaggcgcgag tgctggaatt ggcgtgcggt cccggtggtg tcggcctcgc
     9541 ggcggcagag atcgtcggac cggacggcga agtggtgtgc tcggacgtcg cgccggaaat
     9601 gacggagatt gcggcgcagc gagcggccgc ccgcaacctc cgcaacgtca ggaccgcgca
     9661 gatcggaatg gaggacatcc cattacccga cgcgtcgttc gacgcagtgt tgtgccgaga
     9721 gggattgatg ctggtgatcg accccgccgc cgcagtccga gaggcacacc gtgtgctggt
     9781 accgggtgga cgagccgtgt acgcggtatg ggggccgcgg gatcgcaatc cgtggctggg
     9841 cgtgttactc gacgcgatca ccgcgcacct cggaataccc gtgccgccgc cgggcatgcc
     9901 ggatcctttc gcgctgtcgg actacggccg gctcgaggag ctgctgggcg aagccggatt
     9961 ccgggaggtc agcatccgtg aagtggcgac accgatgaac gcgtcgtcgt tcgacgagtg
    10021 gtgggccgtg gtgccgtcac tggccggccc cgtaggcccg ctgctggaag cgcaacctcc
    10081 cgaggtcagc gcggcgatcc gcggcgaggc cagcgctata ttggacgagt tccgcaccga
    10141 cagcggctac gacctaccgg gcctgagttt cgtcggcgtc ggccaccact aacccaaggt
    10201 ggtcgcgcag ggtgcggccg ctgtattcgg tgcggtaggt accccgctcc tgcagcagtg
    10261 gcaccacctt gtcgacgaac tcgtcgagcc cgtggggagt caggtgcgga acgaaaatga
    10321 acccgtcaca cgcatcggat tgcacgtagt ggtcgatgtc ggcggcgact tcgcctggcg
    10381 taccatcgaa ttgctgccgc ggcggaagtt ctcgccggtg aacgcgtccg aggacgtcac
    10441 catgttccac g
//
LOCUS       DASWBF010000547         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_319166,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000547 DASWBF010000000
VERSION     DASWBF010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319166"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VF874_20510"
     CDS             complement(<1..1133)
                     /locus_tag="VF874_20510"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9178351.1"
                     /translation="MTDTFDVVIVGAGISGISAAYRIRKRNPGISYVILERRANIGGT
                     WDLFRYPGIRSDSSIFTLSFPWEPWTRREGIADGDHIREYMTDTARKHGIYEHIHFDS
                     YVRSADWDSETDTWTVHADDGTTYQARFVFFATGYYNYDEGYTPDFPGIDNFKGDVVH
                     PQHWPEDLDYTGKTMVVIGSGATAVTLVPSLSERAGKVIMLQRTPSYVGTFPRTDSMT
                     EGLRKVLPGRLFHAVARFRMALQEAIAWFLARKTPAFMKYLFRRMQTKRLPEGYDIDT
                     HFSPPYQPWDQRICLAADGDLFDAIDAGRVEVVTDHIDHFDASGIALTSGRHLDADIV
                     VTATGLQLQALGGVALSLDGAEVKPQDRFVYKAHMLQDVPNLF"
     gene            1183..1800
                     /locus_tag="VF874_20515"
     CDS             1183..1800
                     /locus_tag="VF874_20515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9178352.1"
                     /translation="MGMSEAQQAWEEHYGERERVWSGRVNVRLAEVVEALTPGRALDL
                     GCGEGADALWLAEHGWHVTAVDISQTALDRAAAEAKARNASIDFQRHDLTESFPDGVF
                     DLVSAQFLHSTVPMDRAKLMRRAADSVAPGGTLLIVDHAAAPPWASKMHHHEFPSARE
                     VVLSLNLGDSQWDRIRVEAVERDSIGPEGEHAVLTDNVMVLRQRS"
     gene            complement(1788..2240)
                     /locus_tag="VF874_20520"
     CDS             complement(1788..2240)
                     /locus_tag="VF874_20520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX9178353.1"
                     /translation="MTDFSSFSSLPQADQDAIGRTLGALLTGFKTRDVDALAGVYAED
                     ADWVNAFGTVKHSRDGILDHLRGLFKDANFNRGELVGPPEVSFRVLTPEVVLVSAHLK
                     VEGQGLVGGGTLDRDNFSLRAMQRQPDGSWLIVSEMFQDANTETTYER"
     gene            2277..3503
                     /locus_tag="VF874_20525"
     CDS             2277..3503
                     /locus_tag="VF874_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9178354.1"
                     /translation="MSQPPLGANADEVFAWWSSLPADERDRLIAQHPPELGNLNGIPA
                     DVRDQVNLAVLDDDLNRPEDDVRRRNAEQVKRGLDHDRGADPGNQRPVLLWAYDPLAF
                     DGQGRAAIAIGNPDHAEDIAVVVPGAGSSVASGWLHGGHNDAINLYDQSKAAYPDDDL
                     SVIAWMGYDAPDGYRDPRVASPWLAREGGLQLAQDVNGLRATHQGPHPHVTVLGHSYG
                     STTVSDALAASGARANDVILLGSPGTDLAHSAKDFHVDGGNVYVGSASSDPVSWLGEA
                     GGVPNELNEALGYPFGRYAGLGTDPAGDGFGAIRFKAEVPGGDGLSSQNHSHYYDLGG
                     EPLRAMVHVVTGHADALARDDLLARGRRQPHITTPSEVKIPGRGPIRLPHIDTRIPGI
                     PAFIDPEAERPREGSP"
     gene            3500..3931
                     /locus_tag="VF874_20530"
     CDS             3500..3931
                     /locus_tag="VF874_20530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178355.1"
                     /translation="MTLSDEQAMAQVVEAARQIVRAARLQHVTGGFLFESCNDQGSPP
                     YRGRVDMSFAIPQGVEPAAHFERIGATLVRKGWREGPPAGKCPFGVAVHTDTVMAVIC
                     RASGDTTRGAVQVCGECGNMTDHRHDGKTVGVEISDLLTDS"
BASE COUNT          675 a         1213 c         1436 g          640 t
ORIGIN      
        1 cagaacaagt tcgggacgtc ctggagcatg tgcgccttgt agacgaagcg gtcctgcggc
       61 ttgacttccg cgccgtcaag actcaacgcg accccgccga gcgcctgcag ttgcaggccg
      121 gtggcggtga cgacgatgtc ggcgtccaga tgccggcccg acgtgagtgc gatgccgctg
      181 gcgtcgaaat ggtcgatgtg gtcggtgacg acctcgacgc gcccggcgtc gatcgcgtcg
      241 aacaagtcgc cgtcggccgc caggcagatc cgctgatccc acggctggta cggcgggctg
      301 aagtgcgtgt cgatgtcgta gccctccggc agccgcttgg tctgcattcg gcggaacaag
      361 tacttcatga aggctggcgt cttgcgtgcg aggaaccagg cgatcgcctc ctgcagcgcc
      421 atccggaagc gcgccaccgc atggaaaagc ctgccgggca atacctttcg caagccctca
      481 gtcatcgagt cggttcgcgg aaaggtgccg acgtagctag gcgtgcgctg cagcatgatc
      541 actttgcccg cccgctcgga cagcgacggg acgagggtga ccgcggtcgc accgctgccg
      601 atgacgacca tcgtcttgcc ggtgtagtcc agatcttcgg gccagtgctg cgggtggacg
      661 acgtcacctt tgaagttgtc gatgccaggg aagtcggggg tgtagccctc gtcgtagttg
      721 tagtagccgg tggcgaagaa cacgaaccgc gcctgatacg tcgtgccgtc gtcggcgtgg
      781 acggtccagg tgtcggtctc cgaatcccag tcggccgagc ggacgtagct gtcgaagtgg
      841 atgtgctcgt agatgccgtg cttgcgagcg gtgtcggtca tgtactcgcg gatgtggtcg
      901 ccgtcggcga tcccttcgcg cctggtccac ggctcccacg ggaagctgag cgtgaagatg
      961 ctgctgtcgg agcggatgcc ggggtagcgg aacaggtccc aggtgccgcc gatgttggcg
     1021 cggcgctcga ggatgacgta ggagatgccg gggttgcgct tgcggatccg gtacgcggcg
     1081 ctgatgccgg agatgccggc gccgacgatg acgacgtcga aggtgtcggt catcggacca
     1141 gtgtgccgca ggccttcgtc tcggccgtgg caaggtggcg gtatggggat gtctgaggcg
     1201 caacaggcgt gggaagagca ctacggcgaa cgcgagcggg tgtggagcgg acgggtcaac
     1261 gtccggctgg ccgaggtggt cgaggcgttg acgcccggcc gggcgctcga cctcggctgc
     1321 ggggagggcg ccgacgcgct atggctggct gaacacggct ggcacgtgac ggccgtcgac
     1381 atctcgcaga cggcgctgga ccgggcggcg gccgaagcga aggcgcgcaa cgcgagcatc
     1441 gactttcagc gacacgacct tacggagagc ttccccgacg gtgtcttcga cctggtgtcg
     1501 gcgcagttcc tgcattccac ggtgccgatg gaccgtgcaa agctcatgcg ccgcgcggcc
     1561 gactccgtcg caccgggagg caccctgctg atcgtggacc acgccgcagc gccaccgtgg
     1621 gcgtcgaaaa tgcatcacca cgagtttccc agcgcgcgcg aggtggtgtt gtcgctgaat
     1681 ctcggtgatt cccaatggga tcggatcagg gtcgaagccg tcgagcgcga ctccatcggc
     1741 ccggagggcg aacacgcggt gctcaccgac aacgtgatgg tgctgcgtca gcgttcgtaa
     1801 gtcgtctcgg tgttggcgtc ctggaacatc tcggagacga tcagccacga gccgtcgggc
     1861 tggcgttgca tcgcgcgcag ggagaagttg tcgcggtcga gggtgccgcc gccgaccagc
     1921 ccctggccct cgaccttgag atgcgcggag acgaggacga cctcgggcgt cagcacacgg
     1981 aagctgacct ccggggggcc aacgagctcg ccccggttga agttggcgtc cttgaagagg
     2041 ccacgcaggt ggtcgaggat gccgtcgcgg ctgtgcttga cggtgccgaa cgcgttcacc
     2101 cagtcggcgt cctcggcgta cacaccggcc aacgcgtcga cgtcacgcgt cttgaagccg
     2161 gtcagcaacg cgcccagcgt gcgcccgatt gcgtcctgat ccgcctgcgg tagcgacgag
     2221 aaagacgaga aatctgtcac gccgcgagcg taacgcccga tactcgatac gtgacgatgt
     2281 cgcaaccgcc gctcggcgcc aacgccgacg aggtcttcgc gtggtggagc tcgctgcccg
     2341 ccgacgagcg agatcggttg atcgcgcagc atccgccaga gttagggaat ctcaacggca
     2401 ttcccgcaga tgttcgcgac caagtcaacc tcgccgtcct cgacgacgac ttgaaccgcc
     2461 ccgaggacga cgtgcgtcgc cgcaacgccg aacaggtcaa gcggggactc gaccatgaca
     2521 gaggcgccga cccgggcaat cagcggcccg tattgctttg ggcttatgac cctttggcgt
     2581 tcgacgggca gggccgcgcc gcgattgcga tcggcaatcc tgaccacgcc gaagacatcg
     2641 cggtggtcgt gccgggtgcc ggcagcagcg ttgcgagcgg ctggctgcac ggcgggcaca
     2701 acgacgcgat caacctttac gaccagtcca aggctgccta tcccgacgac gacctgtcgg
     2761 tgatcgcgtg gatgggctac gacgcaccgg acggttaccg cgatccgcgg gtggcatcgc
     2821 cgtggctggc gcgcgagggc gggctgcagc tggcgcagga cgtcaatggg ctgcgggcga
     2881 cgcatcaggg accgcatccg cacgtgaccg tgctcgggca ttcgtacggg tcgacgacgg
     2941 tgtccgacgc gctcgccgca agcggcgcac gcgcgaacga cgtgatcctg ttgggttcgc
     3001 cggggaccga tttggcgcac agcgccaagg actttcacgt cgacggagga aacgtgtacg
     3061 tcggctcggc gtcgtcggat ccggtgagct ggctgggtga ggcgggaggt gtgccgaacg
     3121 agctgaatga agcgctgggg tatccgttcg ggaggtacgc gggactcggg acggatccgg
     3181 cgggcgacgg gttcggggcg atccggttca aagccgaggt tcctggaggc gacgggctga
     3241 gctcccagaa ccattcgcac tactacgacc tcggtggcga gccgctgcgg gccatggtgc
     3301 acgtcgtgac ggggcacgcg gacgcgctgg cgcgtgacga tctcctggcc cgaggtcgac
     3361 ggcagccgca catcaccaca ccgagtgagg tcaagatccc aggccggggc ccaattcggc
     3421 tgccgcacat cgacactcgc atcccgggca tcccggcgtt catcgatcca gaagccgaaa
     3481 ggccgaggga gggctctcca tgacgctcag cgacgagcag gcgatggccc aggtggtcga
     3541 ggcggccaga cagatcgtcc gcgccgcgag gctgcaacat gtgacgggcg ggttcctgtt
     3601 cgagtcgtgc aacgaccagg gttcgccgcc gtatcgcggt cgggtggaca tgagcttcgc
     3661 gatcccgcaa ggcgtggagc cagcggcgca cttcgagcgg atcggtgcca cgctggtgcg
     3721 aaagggctgg cgcgaaggac cgccggcggg aaagtgcccg ttcggagtag cggtgcacac
     3781 cgacacggtg atggctgtga tctgccgggc gtcgggggac accacgcggg gtgcggtgca
     3841 ggtgtgcggc gagtgcggca acatgaccga ccatcgtcac gacggaaaga cggtcggggt
     3901 ggagatcagc gatctgctta ccgatagtta acgaactgca gggcgacgct cggcatcagc
     3961 tggt
//
LOCUS       DASWBF010000548         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_182027,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000548 DASWBF010000000
VERSION     DASWBF010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182027"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1708)
                     /locus_tag="VF874_20535"
     CDS             complement(242..1708)
                     /locus_tag="VF874_20535"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9178356.1"
                     /translation="MPDSVSHEQLSAQNAELRAENAELKRMITVLTDRIAELERRLSA
                     DSSNSSRPPSSDAPWDKKPARKRSSRTWSARRPGKQPGAPSTSRRLTDDPDKIVVLEP
                     QRCAGCDAPLARAAEQGRERRQVVDVPEPPPLEITEYQRISKICSCCGVVTTPEWETG
                     DENAATVATPGSPVRIGPEVCARAALLTCAHYLPVGRSRQLLEALTAIDVSTGFLAGV
                     CGRAARRLEKQFLGHMRKLLATAPVLHADETTGRAAAALSYVHVACTEYLTLMHVGGR
                     SADDIDAGGVLTAFTGVLVRDGYAGYTHLPAVHAWCAAHLLRDLRSISDADTDGQLWA
                     TAMAGVLIDTNRAAGDARERGADTLDAQTLAGIRNHYLGALAHGNDENRDHNTELAAK
                     ARTLITRFRRYEDMILRFATDLTVPFTNNEAERALRPIKVHQRTSGGCWRPLQGLTDF
                     AVVHSYLDTANKWGINKLDALRQLFTTGAWLPPALHPS"
     gene            complement(1808..1947)
                     /locus_tag="VF874_20540"
                     /pseudo
     CDS             complement(1808..1947)
                     /locus_tag="VF874_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612244.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
BASE COUNT          332 a          673 c          731 g          368 t
ORIGIN      
        1 tgcggtttgt tcggtgttag gcggtgttag gcccgtccgg gaatgagagt gcaccggtgg
       61 ctggctggta tccgcgcggt gacccgacag cctggtcaag tccaggggcg tggtgtacgt
      121 cgtcgtcgtg tgattcttcg attctgtggg tcaggcggtc agtgccgcgg gagtgtcctc
      181 ctgttcggtg tttgtcggat gccataatcg tcccgacgta tggggggctc atcgtagcta
      241 ttcagctggg gtgaagtgct ggaggtagcc atgcgccggt ggtgaagagc tggcggaggg
      301 cgtcgagttt gttgatgccc catttgtttg cggtgtcgag gtaggagtgg acgactgcga
      361 agtcggtgag gccttgcagg gggcgccagc agccgccgga ggtgcgctgg tgaaccttga
      421 tgggccggag ggcgcgttcg gcttcgttgt tggtgaatgg cactgtcagg tcggtggcga
      481 agcgcaggat catgtcttcg tatcggcgga accgggtgat cagggtgcgc gccttcgcag
      541 cgagttcggt gttgtggtcg cggttttcgt cgtttccgtg ggcgagcgca ccgaggtagt
      601 ggttacggat gccggcgagg gtctgcgcgt cgagtgtgtc ggcgccgcgt tcgcgcgcgt
      661 cgccggcggc ccggttggtg tcgatcagca ccccggccat ggcggtggcc caaagctggc
      721 cgtcggtgtc ggcgtcggag atcgaccgca ggtcacgcag cagatgcgct gcgcaccaag
      781 cgtgtacggc gggcaggtgg gtgtagccgg cgtagccgtc gcgcaccagc accccggtga
      841 acgcggtcag cacgccgccg gcgtcgatgt cgtcggcgga gcggccgccg acgtgcatca
      901 acgtcaggta ctcggtgcac gcgacgtgca cgtaggacaa ggctgcggcg gcgcggccgg
      961 tggtttcgtc ggcgtgcagc accggagcgg tcgcgagcaa tttccgcatg tggccgagga
     1021 actgtttctc cagccgtcgg gcggcgcggc cgcagacccc ggccaggaac ccggtcgaca
     1081 cgtcgatcgc ggtcaacgcc tccagcagtt gccgggaacg gcccaccggc agatagtgtg
     1141 cgcaggtcag cagcgccgcc cgcgcgcaca cctcgggccc gatccgcacc ggcgagcccg
     1201 gcgtcgcgac ggtggcggcg ttctcgtcgc cggtctccca ctccggcgtg gtgaccacac
     1261 cgcagcacga gcagatcttc gagatccgct gatactcggt gatttccagg ggcggcggtt
     1321 ccggcacgtc gacgacctgg cgccgctcgc ggccctgctc ggccgcccgc gccagcggcg
     1381 catcacagcc ggcgcagcgt tgcggctcga gcaccacgat cttgtccgga tcgtcggtca
     1441 ggcgccgcga ggtcgacggc gcgcccggct gcttgcccgg cctgcgggcc gaccaggtcc
     1501 gcgacgaccg cttgcgcgcc ggcttcttgt cccacggcgc gtccgaggac ggcggccgcg
     1561 aggagttcga cgaatccgcc gacagtcgcc gctccaactc cgcgatccga tcggtcagca
     1621 ccgtgatcat gcgcttcagc tcggcgttct ccgcgcgcag ctcggcgttc tgcgctgaaa
     1681 gctgctcatg tgacacggaa tcgggcacga acacaccttg cccgccactg cgccaagatc
     1741 aacgcgacac gccgcaccaa gtcacatcaa ccacaccaat cacacgagcc ccagctgaat
     1801 agctaccgaa gaacggcacg aacggggtga agaacgccgg gtcgtccaac agcgcatcga
     1861 cgcgagccaa ctcctcgggc agccgccgca actcttcggg tagaaccgac cccacaatga
     1921 gcactgatcg cctacagtac gaaacacgat ggcctcaatc ccttccacca cagaggcttt
     1981 gaggccatct tcccaggtca acgccaacaa accgcggatc aacgcgccga ctttttcagg
     2041 tcgaagtagc tagttgacga actcgacttg ctctcgtcgt aaccgcagcc cggcgctgtc
     2101 ctca
//
LOCUS       DASWBF010000549         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_845647,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000549 DASWBF010000000
VERSION     DASWBF010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845647"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VF874_20545"
     CDS             <1..402
                     /locus_tag="VF874_20545"
                     /inference="COORDINATES: protein motif:HMM:NF040132.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178357.1"
                     /translation="SHRKEISMPAYDHFYTTSASERDNAIANLGYQDEGTACYLYGAP
                     TGTVPLYRLVKEGHFYTTDLAERDNAITIDGFHSEGIACHVFDTAKAGTVPLYRLLGV
                     GVMAGTQRDSTGTGGVVGGHALAMTSHEIRG"
     gene            405..1994
                     /locus_tag="VF874_20550"
     CDS             405..1994
                     /locus_tag="VF874_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9178358.1"
                     /translation="MDIRCDYVVVGTGSAGAVIASRLSADPATEVVALEAGPRDTNRF
                     IGIPAAFSKLYRSEVDWDYLTEPQKELNGRQIYWPRGKVLGGSSSMNAMFWVRGFGAD
                     YDEWAAQAGPEWSFAEALAYFRRIENVTAASHLVSRDHSGVTGPLNVSPQRSPRHMTA
                     AWLAAVQACGFPAAQPNSPEPEGFCETLVTQRRGARWSTADAYLKPAMRRKNLSVLTE
                     ATATRVIIDGTRAVGVEFDQAGQHGVARARREVVLCGGAINSPQLLMLSGIGDRDHLG
                     DHGIDTVYHAPEVGKNLLDHLATPLGFDVEDDSLFAAEKPKQLINYLLRRRGMLTSNV
                     GEAYGFVRSRPELDLPDLELVFAPAPFYDEGLPAEPPGHGVAFGPILLKPESRGQITL
                     RSANPHAKPIIDPRYLSDPGGVDRTAMMSGLRICARIAQAPTLKGLLGGIARPRGATA
                     LDDATLEEALNTCSHTFYHPVGTCRMGSDDSSVVDPQLRVRGVDGLRVADASVMPSII
                     RGHTQAPSMLIGEKAADLIRG"
BASE COUNT          424 a          791 c          727 g          354 t
ORIGIN      
        1 agtcatcgga aggaaatcag catgccagcc tatgaccact tctacaccac atcggcgtcc
       61 gagcgggaca acgcgatcgc caacctcggc tatcaggacg agggcacggc ctgttatttg
      121 tacggcgcac caacgggcac cgtgccgctg tatcggctgg tcaaggaagg ccacttctac
      181 accacggatc tggccgaacg cgacaacgcg atcaccatcg acggtttcca ctccgagggc
      241 atcgcctgcc acgtcttcga caccgcaaaa gcgggcaccg taccgctata ccgactgctt
      301 ggcgtcggcg tcatggcagg gacgcagcgt gattcaacag ggactggcgg tgtcgtcggc
      361 ggccatgctc tggccatgac ttcacacgaa ataaggggtt gatgatggac atacgctgcg
      421 attacgtcgt ggtcggaacc ggctcagcgg gcgcggtcat cgccagccgg cttagcgctg
      481 atccggcgac cgaggtggtg gcgctggagg caggaccgcg ggacacaaac aggttcatcg
      541 gtatcccggc agcgttctcg aagttgtacc gcagcgaggt ggactgggat tatctgaccg
      601 aaccgcagaa agaactcaac ggccgccaga tctattggcc ccgaggcaaa gtcctcggcg
      661 gctcatcgtc aatgaacgcc atgttctggg tgcggggatt cggggccgac tatgacgagt
      721 gggccgcgca ggccgggccg gagtggtcct tcgcggaggc gctggcgtac tttcgccgca
      781 tcgaaaacgt caccgctgcc agccacctcg tcagccgcga ccacagcgga gtgacgggcc
      841 cactgaacgt gtcacctcag cgcagcccgc gacacatgac cgcggcctgg ctggcagcgg
      901 tgcaggcatg cggatttccc gccgcgcaac cgaactctcc tgagccggag ggcttttgcg
      961 aaaccctcgt cacgcagcgc cggggcgcga ggtggagtac cgccgacgcc tatctcaagc
     1021 cggcgatgcg ccgcaaaaac ctcagcgtgc tcaccgaggc cacggcgacc cgagtcatca
     1081 tcgacggcac acgggcggtc ggtgtggaat tcgaccaggc cggccagcac ggcgtcgccc
     1141 gcgcccgccg cgaagtggtg ctctgcggcg gcgccatcaa cagcccgcaa ctgctgatgc
     1201 tctccggcat cggggatcgc gaccacctcg gcgaccacgg catcgacacc gtctaccacg
     1261 cccccgaggt ggggaagaac ctgctcgacc acctcgccac accgctgggc ttcgacgtcg
     1321 aagacgacag cctattcgcg gccgagaagc cgaaacagct gatcaactac ctgctgcggc
     1381 gccgcggcat gctcacctcc aacgtcggtg aggcctacgg gtttgtccgc agccgacccg
     1441 agctggacct gcccgacctc gagctggtct tcgctcccgc accgttttac gacgaggggc
     1501 tgcctgctga gccgccgggg cacggcgtgg cgttcgggcc gatcctgctc aaaccggaaa
     1561 gccgcggcca gatcacgctg cggtccgcca acccgcacgc caagccgatc atcgacccgc
     1621 gctacctgtc cgatcccggc ggcgtcgacc gcaccgcgat gatgtcgggc ctgcggatat
     1681 gcgcccggat cgcccaggcc cccacgctga aaggtctact cggcggtatc gcacgacccc
     1741 ggggcgccac ggcgctggac gacgccaccc tcgaggaggc cctcaacacc tgctcgcaca
     1801 ccttttatca cccggtcggc acctgccgga tgggcagcga cgactcaagt gtggtcgatc
     1861 cgcagctgcg agttcgcggg gtcgatggcc ttcgggtcgc cgacgcgtcg gtgatgccca
     1921 gcatcatccg cggacacacc caggcgccgt cgatgcttat cggggagaag gccgccgatc
     1981 tgatccgcgg ctgacggctg gcaccgaatc cgcagcaggc tagcttggtt cgcacgcgat
     2041 cccaacacgc tgaccggtcg cctcgatgag tggcgaaccg ttcgcgcaaa ggccgacccc
     2101 gacggcgtct tcatgtctga ccttgcccgt cgacttgaac ttattgccta gctagaacca
     2161 tgcggggcat tgcgatgtca accaggaacg tccccggcgt cagccccgca agcattggcg
     2221 cagggtcccg aaaccgtggc tgacgtagac cggcgccgcg agctgctcga cgccgcggcc
     2281 cgagaccggt caggag
//
LOCUS       DASWBF010000550         5590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_319433,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000550 DASWBF010000000
VERSION     DASWBF010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319433"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VF874_20555"
     CDS             <1..519
                     /locus_tag="VF874_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9178359.1"
                     /translation="GIYFWFPKMTGRMLDERMGRLHFWLTLIGFHTTFLVQHWVGNEG
                     MPRRYADYLPTDGFETLNIVSTIGSFILGASMIVFTWNVFRSWRYGAPVTVDDPWGYA
                     NSLEWATSCPPPRHNFTELPRIRSERPAFELHYPHMIERMRAESHIGGRREKHLETDD
                     AQTSLRTAPDRE"
     gene            complement(532..2460)
                     /locus_tag="VF874_20560"
     CDS             complement(532..2460)
                     /locus_tag="VF874_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9178360.1"
                     /translation="MGRHSIPGPDDSPREPFEATPAPQHEPFEEHYPSYEPVDEQHRL
                     ETSAAPPSPEDEYSGGRFADGTWQGGHRSTDRKRRGVSVAVIVALFAVVVLVGGIILW
                     RFFGNALSNRSDTAAAACSGSDVPVAVIADPSIADKITEFADRYNKSADPIGDRCVKL
                     AVQAAGSNQVIDGFVGNWPSDLGQRPALWIPASSVSTSRLQAAAGSNVITDSRSLVTS
                     PVVLAIKPQLKTALASQNWGTLPGLQTNPASLEAMNMPGWGSLRLALPTDGDSDASYL
                     AAEAVAAASAPPNEPPTAGVGAVNTLIAGQPKLSNPSLSAAMDALMTTGDPAAAPVHA
                     VVITEQQLYQRSAKVPDAKDVVSSWLPPGPAVVADYPMASLAGDWLTEEQMTAASEFK
                     KFLQKPEQQAELAKAGFRAENQSLPKSDVAGFSPLSGTLSIGDDERVTLADAVTAPAT
                     GGAVTIMLDQSMSEQEGGKSRLANVVAALRNKLQALPPNAVVGLWTFDGVEGRSEVAA
                     GPLSDQVNGQPRSAALTAALDKQYSSNGGAVSFTTLRLIYAGALANFRPGMKNSVLVI
                     TSGPHTDQSLDGAGLQDYVRKTFDPTRPVAINVIDFGADPDRGAWESVAQATGGSYQN
                     LTTSDTPDLTTAVSTFLG"
     gene            complement(2491..4671)
                     /locus_tag="VF874_20565"
     CDS             complement(2491..4671)
                     /locus_tag="VF874_20565"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX9178361.1"
                     /translation="MTERVATGNLRVAKVLYDFINNEALPGTDIDPDTFWAGVDKVVA
                     DLGPRNQELLARRDELQGQIDKWHRHRVIEPLDQEAYRQFLVDIGYLRPEPEDFAVTT
                     ANVDAEITSTAGPQLVVPILNARFALNAANARWGSLYDALYGTDVIPEDDGAEKGDSY
                     NKVRGDMVIAYARRFLDDSVPLANGTYTDVTGFKVDDGRLIVTLDDERETALAQPQQF
                     VGYTGDPGSPDSVLLVNHGLHIDILIDPESPIGKTDEAGVKDVYLESAVTTIMDFEDA
                     VAAVDADDKVKGYRNWLGLNKGDLAAEVSKDGDTFTRVLHEDRRYTAPDGSEFALPGR
                     SLLFVRNVGHLMTNDAIVDADGNEVFEGIQDALFTSLIATHGMKSGEANGPMVNSRAG
                     SIYIVKPKMHGPDEVAFTVELFSRIEDVLGLPQSTIKVGIMDEERRTTVNLKACIRAA
                     ADRVAFINTGFLDRTGDEIHTSMEAGPMVRKGTMKSQPWILAYEDNNVDIGLECGLPG
                     HAQIGKGMWTMTELMGDMVEQKIGQPKAGASTAWVPSPTAATLHAMHYHQVDVGEVQK
                     QLAAQGRRAMVDQLLTIPLAKELAWESDEIREEVDNNCQSILGYVVRWIDQGVGSSKV
                     PDIHNVALMEDRATLRISSQLLANWLKHGVITAEDVRASLERMAAIVDQQNEGDQAYR
                     PMAPNFDDSVAFLAAQELILAGTEQPNGYTEPILHRRRREAKAR"
     gene            4833..5171
                     /locus_tag="VF874_20570"
     CDS             4833..5171
                     /locus_tag="VF874_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178362.1"
                     /translation="MDGPLTHKPRRAVGHRRGVAPGGPGRALVVDTFYRVESQPVPAQ
                     RVHQVTGAVAAADASALYAVAYSYAPFHCPDCPASYCGEHWAWRRFDDCDFSGIEGRC
                     PDGHFHVLCY"
BASE COUNT          854 a         1851 c         1864 g         1021 t
ORIGIN      
        1 ggcatctatt tctggttccc gaagatgacg ggcaggatgc tcgacgaacg gatgggccgg
       61 ctgcatttct ggctgacgct gatcggtttt cacaccacgt tcttggtgca gcactgggtg
      121 ggcaacgagg gcatgccgcg ccgctacgcc gactacctgc ccaccgacgg cttcgagacg
      181 ctcaacatcg tgtccaccat cggctcattc atcctgggtg cctcgatgat cgtgttcacc
      241 tggaacgtgt tccgcagctg gcgctacggc gcgccggtga ccgtggacga cccgtggggc
      301 tacgccaact ccctggaatg ggccaccagc tgccccccac cacggcacaa cttcaccgaa
      361 ctaccccgaa tccgctcaga acggcccgcc ttcgaactgc actacccgca catgatcgaa
      421 cgcatgcgcg ccgaatccca catcggcggc cgtcgcgaaa agcatctgga gaccgacgac
      481 gcccagacgt ccttgcggac ggcacctgat cgggagtgag gacgttctcg ctcagcccaa
      541 gaacgtcgac actgccgtcg tgaggtcagg ggtgtccgac gtggtcaggt tctggtagct
      601 ccccccggtc gcttgcgcga cggattccca tgcgccacgg tcgggatcgg cgccgaagtc
      661 gatgacgttg atcgccaccg gccgggtggg atcgaacgtc ttgcggacgt agtcctgcag
      721 acctgcgccg tcgaggctct ggtcggtgtg cgggcccgag gtgatcacca gcactgagtt
      781 cttcatgccc gggcggaagt tcgcgagcgc gccggcatag atcagccgca atgtcgtgaa
      841 cgacacggcg ccgccgttcg acgaatactg cttgtccagc gcggctgtca gcgcggcgga
      901 ccgcggttga ccgttcacct ggtcggagag cggaccggca gccacctcgc tgcggccctc
      961 gacaccgtcg aacgtccaca acccgacgac cgcgttcggc ggcagcgctt gcagcttgtt
     1021 cctcagggcc gcaacgacat tcgccaggcg tgacttgccg ccctcctgct cggacatcga
     1081 ctggtcgagc atgatcgtga cggcgcctcc ggtggccggt gcggtcaccg cgtcggccag
     1141 cgtcacgcgc tcgtcgtcgc cgatcgacag ggtgcccgac agcggcgaga agccggccac
     1201 gtcgctcttc ggcagcgatt ggttttcggc gcggaacccc gctttcgcca actcagcctg
     1261 ctgctccggc ttttgcagga acttcttgaa ctcgctggct gcggtcatct gctcctcggt
     1321 gagccagtcg cccgcgagcg aagccatcgg gtagtcggcc accaccgcag ggcccggcgg
     1381 tagccacgag ctgacgacgt ccttcgcgtc gggcaccttc gccgagcgtt ggtagagctg
     1441 ctgttcggtg attacgaccg cgtgcaccgg agcggccgcg gggtcgccgg tggtcatcag
     1501 cgcgtccatc gcggccgaca ggctcgggtt ggacagcttg ggctggccgg cgatcaacgt
     1561 gttgaccgcg ccgacaccgg cagtgggcgg ttcattcggc ggcgccgacg cggccgccac
     1621 cgcctcggcg gcgaggtagc tcgcgtcgct gtcaccgtcg gtcggcagcg ccaaccgcaa
     1681 tgacccccag cccggcatgt tcatcgcctc cagcgacgcc ggattggtct gcagtccggg
     1741 cagcgtgccc caattctggg acgccagagc ggttttgagt tgcggcttga tggccagcac
     1801 cacgggggag gtgaccagcg accggctgtc ggtgatgaca ttcgagccgg ctgccgcctg
     1861 cagcctggag gtcgataccg agctggcggg aatccacagc gcgggccgct gaccaagatc
     1921 ggagggccag ttgccgacaa agccgtcgat cacttgattg gagccggccg cttggaccgc
     1981 aagcttgacg cagcggtcgc cgatgggatc ggcggacttg ttgtagcggt cggcaaactc
     2041 ggtgatcttg tcggcgatcg acgggtccgc gatcaccgca accggcacgt cgctgcccga
     2101 gcaggcggcc gcggcggtgt cggagcggtt cgacagcgcg ttcccgaaga accgccagag
     2161 gatgattccg ccgaccagca ccacgaccgc gaacagcgcg acgatgacgg cgacgctgac
     2221 gccgcggcgt ttgcggtcgg tgctgcgatg gccaccctgc caggtgccgt cagcgaagcg
     2281 gccgccggag tactcgtcct ccggcgaggg cggggctgca gacgtctcga ggcggtgttg
     2341 ttcgtcgacg ggttcgtacg acgggtagtg ctcctcgaaa ggttcgtgct ggggcgcagg
     2401 ggtagcctcg aacggctccc tggggctgtc gtccgggccg ggaatgctgt gtctacccac
     2461 tgtgctgtct cttcttcgcg caaacgctca tcagcgagcc ttggcctcgc gtcggcggcg
     2521 gtgcaggatc ggctcggtat agccgttggg ctgctcggtg ccggccaaaa tcagttcctg
     2581 ggcggccagg aacgcgacgc tgtcgtcgaa gttcggcgcc atcggccggt acgcctggtc
     2641 accctcgttc tgctggtcca cgatcgcggc catccgctcg aggctggcgc ggacgtcttc
     2701 ggcggtgatc acgccgtgct tgagccagtt ggccagcagt tgactcgaaa tacgaagtgt
     2761 cgcacggtct tccatcagcg ccacattgtg gatgtcgggc accttcgacg aaccgacacc
     2821 ctggtcgatc cagcgcacca cataacccag gatcgactgg cagttgttgt ccacctcttc
     2881 gcggatctca tcggactccc aggccagctc tttggcgagc ggaatggtta gcagctggtc
     2941 taccatcgcc cgccgcccct gcgcggcgag ctgcttctgc acctcgccca cgtcaacctg
     3001 gtggtagtgc atcgcgtgca gcgttgcggc ggtgggcgac ggcacccacg cagtggacgc
     3061 gccggccttg ggctggccga tcttctgctc gaccatgtcg cccatcagct cggtcatcgt
     3121 ccacatgccc ttgccgatct gggcgtggcc gggcagcccg cattccagac cgatgtccac
     3181 gttgttgtcc tcgtacgcca ggatccacgg ctgcgacttc atcgtgccct tgcggaccat
     3241 cggcccggcc tccatggagg tgtggatctc gtcgccggtg cggtccaaga agcccgtgtt
     3301 gatgaacgcc acccggtcgg ccgcggcccg gatgcaggcc ttgaggttga cagtggtgcg
     3361 ccgctcctcg tccatgatcc cgaccttgat cgtggactgc ggcaaaccca gcacgtcctc
     3421 gatgcggctg aacaactcga ccgtgaacgc cacctcgtcg gggccgtgca tcttgggctt
     3481 gacgatgtag atcgacccgg cgcggctgtt gaccatcggt ccgttggcct cgcctgattt
     3541 catgccgtgc gtcgcgatca gactggtgaa cagcgcgtcc tggatgcctt cgaagacctc
     3601 gttgccgtcc gcgtcaacga tcgcgtcgtt ggtcatcagg tgcccgacgt tgcggacgaa
     3661 cagcaggctg cggccgggca gggcgaactc gctgccgtcc ggggcggtgt agcggcggtc
     3721 ctcgtgcagc acgcgggtga aggtgtcgcc gtccttgctg acctcggcgg ccaggtcacc
     3781 tttgttgagc cccaaccagt ttcggtagcc cttcactttg tcgtcggcgt cgacggcggc
     3841 caccgcgtcc tcgaaatcca tgatcgtggt gaccgcagac tcgaggtaca cgtccttgac
     3901 cccggcctcg tccgtcttgc cgatgggcga ctccgggtcg atcaggatgt cgatgtggag
     3961 gccgtggttg accagcaaca ccgagtcggg ggaacccggg tcaccggtgt agccgacgaa
     4021 ctgctggggc tgagcgagcg cggtctcgcg ctcgtcgtcg agcgtgacga tcagccggcc
     4081 gtcgtcgacc ttgaaaccgg tgacgtcggt gtacgtgccg ttggccagcg gcaccgagtc
     4141 gtccaggaat cggcgcgcgt acgcgatcac catgtcgccg cgcaccttgt tgtagctgtc
     4201 acccttctcc gcgccgtcgt cctccgggat cacgtcggtg ccgtacagcg cgtcgtaaag
     4261 cgagccccag cgcgcgttgg cggcgttcag cgcgaaccgc gcgttcagga tcggtacgac
     4321 gagctggggc ccggccgtcg acgtgatctc ggcgtccacg ttggcggtgg tgacggcgaa
     4381 gtcctcgggt tcgggccgca gataaccgat gtcgacgagg aactgccggt aggcctcctg
     4441 gtcgagcggc tcgatcaccc ggtgtcggtg ccacttgtcg atctggcctt gcagctcgtc
     4501 gcggcgcgcc agcagctcct ggttgcgggg gcccaggtcg gcgaccacct tgtccacgcc
     4561 cgcccagaac gtgtcggggt cgatgtcggt gccgggcaag gcctcgttgt tgatgaagtc
     4621 gtacagaacc ttggccaccc gtagattgcc ggtggccacc cgctccgtca tgcgtcctcc
     4681 cgaagtttgt gtcggtacag cttacccagc agaacgtcgg gtttttcggc acggcaccgg
     4741 agaggaggct cggtggcgcg cgaagcgagt ttctcctgcg tggtgtgcga cgagccagcg
     4801 ggacgggtgc ggctgctggc gggaagcgaa ccgtggacgg accgctcacg cacaagcctc
     4861 gacgcgctgt gggacatcga cgcggcgttg cgcccggcgg accaggccgc gcgctcgtcg
     4921 tcgacacgtt ctaccgcgtc gagagccagc ccgtgcccgc ccagcgcgtc caccaggtga
     4981 ccggggcggt cgccgcggcc gacgcgtcgg cgttgtatgc ggtcgcctac agttacgccc
     5041 cgtttcactg cccggactgc ccggcgtcct actgcggtga gcactgggcg tggcggcgct
     5101 tcgacgactg cgatttcagc ggcatcgagg gccgctgccc ggacggtcat ttccacgtgc
     5161 tgtgctactg agccgccgcc gtcgcatggg tcttggcgag tcacatcagc cacttgggtc
     5221 accggcctcg gcggtatccg aattgcccag ctctaagcga catccgctga gctgcgggca
     5281 aggtgcaagt tgtcgctgag cactccaccc tcttcggctc cacagcctgg gacaacgcaa
     5341 tttgtcgctt agagccgggc acaagcatct tcaatcccgc gagagacgag tggggttagt
     5401 gagacagcgc aggaaatctg acattcttcg gcgcgaaacc ccgggcgacc cgatagttga
     5461 acttctgtct cgccattcca gaactggcgt tcatgcaccg gtggcgagaa cgtgtactcc
     5521 atcgaggtcg agcgggcgat cgcggccctc gacgcggtcg ccgaggtcgc ggtgatcgga
     5581 attcccgacg
//
LOCUS       DASWBF010000551         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1107571,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000551 DASWBF010000000
VERSION     DASWBF010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107571"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /locus_tag="VF874_20575"
     CDS             complement(<1..1882)
                     /locus_tag="VF874_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9178363.1"
                     /translation="MARRGHGRASRYSAYTGGPDPLAPPVDLRDALEQIGRDVMEGSS
                     PRRALSELLRRGNKDMRGTDRLAAEANRRRRELLSQHNLDGTLQEVKRLLDEAVLAER
                     KELARALDDDARFSELQLESLPPSPAKAVQELADYQWRSGEAREKYEQIKDLLGREML
                     DQRFAGMKQALENATDEDRQRVNEMLDDLNELLDKHARGEDTQQDFENFMAKHGEFFP
                     ENPQNVEELLDSLAKRAAAAQRFHNSLSPDQRAELDALAQQAFGSPSLQQALGRLDAH
                     LQAARPGEDWTGSSRFSGDDPLGMGQGTQALSDIAELEQLAEALSQSYAGATMDDVDL
                     DMLARQLGDEAAVDAQTLAELERALLNQGFLDRGSDGQWRLSPKAMRQLGETALSDVA
                     RQLSGRHGERETQRAGAAGELTGATRPWQFGDTEPWNVTRTLTNAVLRQAGSGNLAEG
                     RSSHIRVTVDDVEVSETETRTQAAVALLVDTSFSMVMENRWLPMKRTALALNHLVSTK
                     FRSDALQIIAFGRYARTMSAAELTGLEGVYEQGTNLHHALALAARHLRRHPSAQPVVL
                     VVTDGEPTAHLEGGFGRGDEPAQVFFDYPPHPRTIAHTVKGFDEVANLGAQVTIFRLG
                     NDP"
     gene            complement(1875..>2137)
                     /locus_tag="VF874_20580"
     CDS             complement(1875..>2137)
                     /locus_tag="VF874_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX9178364.1"
                     /translation="KLLGGIDVGPIVAAVEHGSPVTTGERVSAREVLAALPEVPAVAA
                     IQRRLGATSDGQRAAAIELALEALYLAKRIDKVSGDGETVYG"
BASE COUNT          312 a          770 c          679 g          376 t
ORIGIN      
        1 cggggtcgtt gcccagccgg aagatcgtca cctgcgcgcc gaggttggca acttcgtcaa
       61 agcccttcac cgtgtgcgcg atcgtccgcg gatgcggcgg gtaatcaaag aagacctgcg
      121 ccggctcgtc gccgcgaccg aagccgccct ccaggtgcgc ggtcggctcg ccgtcggtga
      181 ccaccagcac gaccggctgc gcgctcggat gccgacgcag atgccgtgcg gccagagcca
      241 gcgcgtgatg caggtttgtg ccctgctcgt agacgccctc gagcccggtc agctcggcgg
      301 cgctcatcgt gcgcgcgtat cggccgaacg cgatgatctg caacgcgtcc gaccgaaatt
      361 tcgtgctgac aaggtgattc agcgccaacg cggtgcgctt catcggcagc caccgattct
      421 ccatcaccat cgagaacgac gtgtccacca gcagcgccac cgcggcctgc gtacgggttt
      481 cggtctcgga cacttcgacg tcgtcgaccg tgactcgaat gtgcgaggat cggccttccg
      541 ccaaatttcc cgaaccggct tgacgcagaa cggcattcgt cagcgtgcgc gtcacattcc
      601 acggctcggt gtccccgaac tgccagggcc gcgtcgcgcc ggtcaactca cccgccgcac
      661 cggcgcgctg tgtttcgcgc tcgccatgcc ggcccgacag ctgccgggcg acgtcactca
      721 atgccgtctc ccccaactgc cgcatggctt ttggcgacag ccgccactgc ccgtcggatc
      781 cgcggtcgag gaagccctga ttcaacagag cccgctccag ttccgccaac gtctgcgcgt
      841 cgacggcagc ctcgtcgccg agctggcgcg ccagcatgtc gaggtcgacg tcgtccatcg
      901 tcgcgccggc gtagctctgc gacagcgcct cggccagctg ctcgagctcg gcgatgtccg
      961 aaagcgcttg cgtcccttgg cccatcccga gtggatcgtc gccggagaac cgcgacgacc
     1021 ccgtccagtc ctcccccggc cgtgcggcct gcagatgcgc gtccagccgc cccaaggcct
     1081 gctgcagcga aggtgagccg aatgcctgct gcgccaacgc atccaactcg gcgcgctggt
     1141 cggggctcag gctgttgtgg aaccgctgcg ccgcggccgc gcgcttggcc agcgaatcga
     1201 gtagctcctc gacgttttgc gggttctcgg ggaagaactc gccgtgcttg gccatgaagt
     1261 tctcgaaatc ttgctgcgtg tcctcgcctc gggcgtgctt gtccagcagc tcgttgaggt
     1321 cgtcgagcat ctcgttgacg cgctggcggt cctcgtcggt ggcgttctcc agcgcctgct
     1381 tcatgcccgc gaagcgttgg tccagcatct cacgcccgag cagatccttg atctgctcgt
     1441 atttttcgcg ggcttcgccg ctgcgccact gatagtcggc gagctcttgg acggccttcg
     1501 ccggcgacgg cggcaacgat tccagctgca gctcggagaa ccgggcgtcg tcgtcaaggg
     1561 cgcgggccag ctccttgcgt tcggccagca ccgcctcgtc gagcaggcgc ttgacctcct
     1621 gcaaggttcc gtcaaggttg tgctgggaca acaactctcg acgacggcgg ttcgcctcgg
     1681 cggccagccg gtcagtgccg cgcatgtctt tgttgccgcg ccgcagcagc tccgagagtg
     1741 cgcgccgcgg cgagctgccc tccatcacgt cgcggccgat ctgctccagc gcgtcacgca
     1801 gatctaccgg cggcgccagc ggatcggggc caccggtgta ggcggagtac cgggaggccc
     1861 gcccatgccc tcgtctagcc atatacggtc tccccgtcgc cgctgacctt gtcgatccgc
     1921 ttggccagat acagcgcctc gagcgccaat tcgattgcgg cagcacgctg tccgtcggac
     1981 gtggcgccca agcgccgctg aatggccgcg accgccggga cttcgggcag cgccgcaagg
     2041 acttcccgag ccgacacccg ttcaccggtg gtgaccggtg acccgtgctc gaccgcggcg
     2101 acgatgggcc cgacgtcgat gccgcccaac aacttct
//
LOCUS       DASWBF010000552        26662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_319657,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000552 DASWBF010000000
VERSION     DASWBF010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 26662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26662
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319657"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VF874_20585"
     CDS             complement(<1..542)
                     /locus_tag="VF874_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9178365.1"
                     /translation="MTVYFIGAGPGAADLITVRGQRLLQRCPVCLYAGSIMPDDLLVL
                     CPPDARVVDTGLLTLDQIVAELSAADSAGNDVARLHSGDPSLYSAVAEQWRRLETLGI
                     DYEIVPGVPAFAAAAAVLRRELTVPGVAQTVTLSRVATLSTAMPPGEDLATLSLSGAT
                     LVLHLAAAQIDAVVPELIAGG"
     gene            complement(539..1702)
                     /gene="cbiE"
                     /locus_tag="VF874_20590"
     CDS             complement(539..1702)
                     /gene="cbiE"
                     /locus_tag="VF874_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEX9178366.1"
                     /translation="MIVVIGIGADGTAGLSAQARAELRRARTIYGSRRQLDLLDDSVA
                     AQRREWSSPMMPVLQALPDDAHVLASGDPLLHGVGGTLVRLFGPERVRVLPHVSSVTL
                     ACARMGWSVQDTEVISLVTAEPHTAVRRGGRAVVLSRDAKTPASLARLLVDTGRGDSR
                     VSILEQLGGAAERRRDATARDWVVSPAADIDDLNVVAVQYLPDSRVASVPDEVFEHDG
                     QITKHGVRAVTLAALAARPGEVLWDVGAGSGSIAVEWTRTGTDCTAVAFERDDGRRDR
                     IRRNVVAFGVSVEVRGAAPDAFDGAPDPSVIFVGGGMTQPGLLETCFERLPDDGRLVA
                     NAVTAESEAILAQWYSSLGGELRRFQHYAGEPLGKFTGWRPAMPVTQWAVTKR"
     gene            complement(1811..2554)
                     /locus_tag="VF874_20595"
     CDS             complement(1811..2554)
                     /locus_tag="VF874_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9178367.1"
                     /translation="MGDTDVVVIFGGRSEIGTELAVRLAAGATVVLAARGPQRLDSQR
                     EALQAAGAKAVHVTAFDADDLATHGPLVDSLVAEHGPIATAVLAFGILGDQSRAEDDA
                     AHAVQIVRTDYVAQVSLLTHLANAMRAAGRGRLVVFSSVAGVRVRRANYVYGSAKAGL
                     DGFASGLADALDGSGVQLLLVRPGFVIGRMTQGMTPAPFSSTPQQVAAATARALAKGR
                     REVWVPWVLRPVFFGMRLLPRWVWRRLPR"
     gene            2591..3001
                     /locus_tag="VF874_20600"
     CDS             2591..3001
                     /locus_tag="VF874_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent biliverdin reductase"
                     /protein_id="HEX9178368.1"
                     /translation="MANSATTRLTTEALAFLTERHLAMLTTLRADNSPHVVAVGFTFD
                     PKTHIARVITSGGSQKAVNAQRGGVAVLSQVDGARWLSLEGKASVNTDPEAVRDAELR
                     YAQRYRTPRANPKRVVIEVKVERVLGSSELLDRS"
     gene            complement(3005..4129)
                     /locus_tag="VF874_20605"
     CDS             complement(3005..4129)
                     /locus_tag="VF874_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX9178369.1"
                     /translation="MAFSRFSSDAYARRLHEAAKATTAAGLAGLIVTPGYDLRYLVGA
                     QAQTFERLTALVLPAAGTPTVVVPRLELASLKDSAVTELGLGVRDWVDGQDPYSLVVD
                     ALGGAGVATAVTDSMPALHLLPIAKLLGVTPVLATDVLRQLRMIKDHSEIDALRKAGA
                     AIDRVHARVPEFLVPGRTEADVAADIAKAIVAEGHSDVAFVIVGSGPHGADPHHECSD
                     RVLQPGDIVVVDIGGTYEPGYHSDSTRTYSIGEPNLEVAQHYSVLQRAQQAAVAAVRP
                     GVTAEQVDAVARGVLGQAGLADNFVHRTGHGIGLSVHEEPYIVAGNRLPLRPGMAFSV
                     EPGIYFPGRWGARIEDIVIVTSYGAEPVNHRPHELTVVPV"
     gene            complement(4140..5591)
                     /locus_tag="VF874_20610"
     CDS             complement(4140..5591)
                     /locus_tag="VF874_20610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9178370.1"
                     /translation="MKQLSSLDAQFLAIEDGRRTGHVAALAIHDPSTAPNGTLTCATM
                     MDLLNERMHLLPPLRWRLAEVPFGLDYPYWVVDDDFDMTYHVRESALPSPGGDHQLAE
                     LVSRLHARQLDRARPLWEMYVISGLRNGYVAVYTKIHHALIDGVSGAEITGLLLDVSP
                     RGRPIPPAEPTTPERQPGSLQMWGRALFGVQRYPIRVLRSLPHALPNLEETTLSAVPG
                     VSQTGRLLGRLQRAARPDSHPVIRSPLPAPKTSFNRRVSPNRRFAFGRLELQRVKDVK
                     NAFGVTVNDVVVTICAGAVRRWLLDHKELPEQPLVAQIPVSVRTEEQAGTYGNHVLMM
                     SAPLHTDIADPAARLKATAAGLGEMKERHRALPADLLCDINHFIPPALFNRAARATFA
                     LATSSVGRPTWNVVVSNIPGPQFPLYCGGAQLVANYPVSAIADGLGLNITVMSYDGQV
                     DIGIVADREQVPDVWTLLDGLDLSLTELEDAGR"
     gene            complement(5588..6523)
                     /locus_tag="VF874_20615"
     CDS             complement(5588..6523)
                     /locus_tag="VF874_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEX9178371.1"
                     /translation="MIDDVARLLLRTTSRLGQSLPAVSGLFGRRPELDIGGRVLFITG
                     AARGLGAEIARQAHASGAHVALVGRHLQPLQNLADTLGERAAAFEADVIDADKLQRAA
                     DGAVNAFGAIDVVVANAGIASSSQPVATIDPADFERTVEVDLLGQWRTARTTLPALIE
                     RRGHIMFIGSIYAFFNGVRAAPYAMSKAGVEQLARALRVELAPHGVTAGFAYLGFIDT
                     DLVADAFAQHGAAAIRDAAPGFLTRPIPPSDAAKAVLDGISRRAARVTAPAWVAPMLA
                     MRSFTTAVMDDVLLHNRDVARAVENAETEREASPT"
     gene            6776..7735
                     /locus_tag="VF874_20620"
     CDS             6776..7735
                     /locus_tag="VF874_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEX9178372.1"
                     /translation="MPAPLLLLDGASMWFRSYYGVPASITSPDGRPVNALRGFLDAVA
                     TLITRERPARLVVCDDWDWRPQWRVDRITSYKAHRVAEPNPDGEPDVEEVPDELTPQV
                     DWIIEVLDAFGIPVAGAPGYEADDVLGTLAARERRDPVVVVSGDRDLLQVVTDEPVPV
                     KVLYLGRGLSNATKFGPAEVANRYGVPADRAGPAYAELALLRGDPSDGLPGVAGIGEK
                     SATTLLSQYGSLAAILTAADDPKSKLSKAFRSKLLAARDYLDAAGPVVRVATDAPVKL
                     STKTDALPLAAAHPRKVAKLAEQYGVGSSIGRLQKALDALPAR"
     gene            complement(7736..8473)
                     /locus_tag="VF874_20625"
     CDS             complement(7736..8473)
                     /locus_tag="VF874_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEX9178373.1"
                     /translation="MSGPYDPNQQWSSGQPGQEPSPSEPTQHAGWQPPAYSPQQYPQG
                     QPYSPTEQGAAAQPTQYGQQPYSPTEQGAAAQPTQYGQPQQYQAPQAPQYGQQYPATE
                     GYGQPGQYPGAGAQYGEAPAAFGDAGAEKGSKSSLAVLGAVIGVLAAIIVAVVLVMGF
                     WKPGFFVTTKLDVNKAQQGVKDILTNQTTGYGVDNVSDVKCNGGKNPTVKKGDTFTCD
                     ASVDGTHKQVTVTFQDNNGTYEVGRPK"
     gene            complement(8587..11349)
                     /locus_tag="VF874_20630"
     CDS             complement(8587..11349)
                     /locus_tag="VF874_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEX9178374.1"
                     /translation="MTTSATSELAKFSALLPFTLDPFQVQACSALEGGHGVLVCAPTG
                     AGKTVVGEFAVHLALEAGRKCFYTTPIKALSNQKHTDLVRRYGPEKIGLLTGDVSINS
                     GAPVVVMTTEVLRNMLYADSPALQGLSYVVMDEVHFLADRMRGAVWEEVILHLPDEVR
                     LVSLSATVSNAEEFGGWIQTVRGDTTVVVDEHRPVPLWQHMMVGKRLFDLFDYSGGDR
                     PDNAGRGALVNPMLLQHIANRREADRLLNAAPRGRGRGPRPRLYRTASRPDVISVLDH
                     SALLPAIVFVFSRAGCDAAVGQCLRSSLRLTDDEERGRIIEVIDRRTADLSDADLVVL
                     GYHEWREGLVRGIAAHHAGMLPIFRHTVEELFTAGLIKVVFATETLALGINMPARTVV
                     LERLVKFNGEQHLPLTPGEYTQLTGRAGRRGIDIEGHAVVLWQPDVDPAEVAGLASTR
                     TFPLRSSFLPSYNMTINLVSQMGPHQAHELLERSFAQYQADRSVVGLVRAIERNEKLM
                     RELADELGDDPVLEYARLRAKLSERERVQARSSRLQRHQAANEALSSLRRGDIISITH
                     GRRGGLAVVLEADRDPTDPRPLVLTENRWAGRISSADCSDAAAPLGSMTLPKRVEHRQ
                     PRVRRDVASALRSAAAGLTTPSRRTRRTEPGEDPELRPLRDEIRHHPVHRLGDREAKV
                     RVAERYLRLERENEQLRGKVAAATNSLARTFDRIVGLLTERGFIHQRPNGVLRVTDDG
                     RLLARIYSESDLLVAECLRGGVWRGLNSAELAGVVSAVVYESRGADGPAPMRAGEILT
                     AALRRALADTRRLAVTLRGDEQRHRLDASREPDDGFVTAVYRWATTGDLTAALEASDV
                     AGTGTPSPASGRYPQPAGDFVRWCRQVLDLLDQIRIAAADKGLRTSAKRAIEEIRRGV
                     VAVDGG"
     gene            complement(11346..12248)
                     /gene="tatC"
                     /locus_tag="VF874_20635"
     CDS             complement(11346..12248)
                     /gene="tatC"
                     /locus_tag="VF874_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9178375.1"
                     /translation="MKTPGFLRRLDPRRRRSRVNPGGTMSLVDHIRELRYRLLISVGA
                     VALTTIVGFVWYSHGIFGVESLGEWLRHPYCALPPSARADISADGGCRLLATAPFDQF
                     MLRLKVGLAAGIVLACPVWLYELWAFITPGLYKNERRFAVGFVICAAVLFVSGAVLAY
                     IVLSKALHFLLTVGSDVQVTALSGDRYFGFLINLLLVFGVGFEFPLLIIMLNLIGVLT
                     YTRLKAWRRGLIFGVFVFAAFATPGSDPFSMLALGLALTILLELAIQIARVHDKRKAK
                     REAAEGWAVADDEASAIEPPSKVT"
     gene            complement(12304..12549)
                     /gene="tatA"
                     /locus_tag="VF874_20640"
     CDS             complement(12304..12549)
                     /gene="tatA"
                     /locus_tag="VF874_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEX9178376.1"
                     /translation="MNALQPWHVIVFVVVVIVVFGSKKLPDAARSLGKSMRIFKSEMR
                     ELKHEGNSEDPPKINPVQSERVDTPAPEENHTDARPA"
     gene            complement(12580..13536)
                     /locus_tag="VF874_20645"
     CDS             complement(12580..13536)
                     /locus_tag="VF874_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX9178377.1"
                     /translation="MSQLSTRLVRLLNMVPYFQANPGISAVEAAADLGVSTEQLMTDL
                     NQLWMCGLPGYSPGDLIDLSFSEESIEVIFSAGMDRPLRLTSPEATAMLVALRALQEV
                     PGMVDPQAARSAIAKIESAAGQPQGGAMPDDASEESPAAAAVREAVRLHHAVAIDYYS
                     ASHDTSSHRVVDPIRILFVGDQSYLEAWCRSAEGVRLFRFDRIDSAQVLDEPSAPPPL
                     AVEAGPDTSLFDADPSLPSATLHIAPTASWMFDYFPMRMVRELPDGSCEADMTYASEE
                     WMARLVLGFGAAVKVLAPSSLAAQVQSAATAALQAYEADSLR"
     gene            complement(13533..14522)
                     /locus_tag="VF874_20650"
     CDS             complement(13533..14522)
                     /locus_tag="VF874_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX9178378.1"
                     /translation="MGISKVERLMNLVIALLSTRNFITAERIRKTVLGYADCPTDDAF
                     ARMFERDKNELRDLGIPLETGRVSTLDPAEGYRIKRDAYALPDIALTADEAAAVAVAV
                     QLWQSPELITATQGAVLKLRAAGVDVDAPDISVSIASTSALPGIRGSEPVLPNLLSAI
                     GSGQAVQFQHRSSRVEPYSIRTVEPWGVVTDRGRWYLVGKDRDRDAVRTFRLSRIGAE
                     VTPIGPVGAVRKPDDVDLRDIVAKAVREAPTGVEARIWVADDRATALRRAGAVVESMT
                     LRGRDGDVVAIDIGSVDRLAREVAGYGADAVVLEPESLRDEVIARLRAQAVSA"
     gene            complement(14604..15962)
                     /gene="pafA"
                     /locus_tag="VF874_20655"
     CDS             complement(14604..15962)
                     /gene="pafA"
                     /locus_tag="VF874_20655"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HEX9178379.1"
                     /translation="MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSS
                     NVFLRNGARLYLDVGSHPEYATAECDSLAQLVTHDRAGERVLEDLLIDAEQRLADEGI
                     GGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQT
                     PKAATFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMCET
                     TTMLKVGTAALVLEMIESGVAFRDFSLDNPIRAIREVSHDLTGRRPVRLAGGRQASAL
                     DIQREYHARAVEHLQTREPNEQIEQVVDLWGRQLDAVESQDFAKVDTEIDWVIKRKLF
                     QRYQDRYNLELSDPKIAQLDLAYHDIKRGRGVFDLLQRKGLATRVTTDEEIEEAVDTP
                     PQTTRAKLRGEFISAAQEAGRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDERVKRLIA
                     SM"
     gene            complement(16027..16149)
                     /locus_tag="VF874_20660"
     CDS             complement(16027..16149)
                     /locus_tag="VF874_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178380.1"
                     /translation="MQQAQSELQAIPDKAGHRVAAISLVEQAIDQVNQGIQAGA"
     gene            16301..16429
                     /locus_tag="VF874_20665"
     CDS             16301..16429
                     /locus_tag="VF874_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178381.1"
                     /translation="MARYWPGLSGFAAPPQLDLYARTPYYQGTETFAHVPFGRLDA"
     gene            16484..18157
                     /locus_tag="VF874_20670"
     CDS             16484..18157
                     /locus_tag="VF874_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9178382.1"
                     /translation="MGGRPTLSQAKSWHPDSLRQAADAWDAAAMDIRREFDVVVQGMH
                     DTHDFWVGDAAEAARERAARTGADAARLARALVAAAVAARDGADVIAAARDDVLGTVA
                     AVQSQGFAVTDDGTVTPPAQSPPAVLATAGGNMQIAQEVLRSGAEEFTREIVEKLDAL
                     GAADDDAARDIDEAFHGVTTPQPTAPTGLDDNPVGNWPAMTQDSIAAQIAAMKPEQRR
                     QLIESYPQQVGNTDGVPWDMRVAANRLNIEQAILDGRRELKRTQDPTQRDAVQQRIGF
                     YQGLLSEVPDPTGRKGRVDRQILAFDPGRSSLVELIGDLGTANNVGVLVPGMNTTMEG
                     SAANTETATRFVQAAHGDLAMITYLGGPFPQGGIPDGIDDAMDTDYATGMAPRLVSFS
                     EDVNRVVDATGRDIAVTYVGHSYGGSVLGTAEAMGLTADRTVYVEAAGAGVGVDDLGD
                     WHNENPYVMRFSMTAPGDWIEPLQGMPLNPHGADPDEMPGVIRLATGDYDDGQRMVGW
                     DTHSDVLNEPSDAWRNILAAMTGQPYSVYVDPLKFLPTWAKPPWLRLTG"
     gene            18225..19664
                     /locus_tag="VF874_20675"
     CDS             18225..19664
                     /locus_tag="VF874_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9178383.1"
                     /translation="MQSCDVCIVGAGIAGLNALFVASRYLSRDQKVILVDSRERVGGM
                     WVDTYPYVRLHQPHPMFTAGNIKWTLGQDRSYLATKGEVLDHFEHCLNVIKQRVQVDE
                     FFGWTLESDDEADGIVRITCRSADGGLLVVEANRLIKAYGLRVMPNDPLEISSARVQS
                     VSPDYCDVRSGDMRASDTPVWIIGGGKTAMDTAHALITEYPGRDVNLVAGSGTFFTSR
                     DRLFATGARRWWGGTLGSTIGTEMARRFDGTNEAEVEEWYRGTYGTWLTPSTSNFVLG
                     VLSESEKQTIAAGLNDVIMDHLVDVVDRDGATELMLRSGSTKAIQPGSWIVNCTGYLM
                     ARGHSYEPYVSDSGAVVSIQPRSATLHLPAFMGYFLAHLLFLGKLRDVPLYEIDVEDL
                     RTKSRAVFPYTVFALVQHNLSLIFDSVPSKVFGECGLDFDTWYPLPRRLASTARFMLT
                     HRRVRERQRRVLDTVRERFDVRCGPLTHE"
     gene            complement(19678..20400)
                     /gene="prcA"
                     /locus_tag="VF874_20680"
     CDS             complement(19678..20400)
                     /gene="prcA"
                     /locus_tag="VF874_20680"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEX9178384.1"
                     /translation="MTFPYFISPEQAMRERSELARKGIARGRSVLALAYSGGVLFVAE
                     NPSRSLQKVSELYDRVGFAAVGRFNEFDNLRRGGIQFADTRGYAYDRRDVTGRQLANV
                     YAQTLGTIFTEQAKPYEVELCVAEVAHYGETKPPELYRITYDGSINDEPHFVVMGGLT
                     EPITTALKDSYQENATLAEAVGIAVKALQAGTSGNGSGSSEPRTLGVSTLEVAILDQN
                     RPRRAFRRITGSALESLLPQGD"
     gene            complement(20397..21296)
                     /gene="prcB"
                     /locus_tag="VF874_20685"
     CDS             complement(20397..21296)
                     /gene="prcB"
                     /locus_tag="VF874_20685"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX9178385.1"
                     /translation="MTWPYREQLAIPNPNSGASPLPLDLSSFSEYLRRQAPQLLPVSG
                     AGVSGAAGVQLAHGTTIVALNFPGGAVIAGDRRATQGNMIASRDVKKVYITDDYTATG
                     IAGTAAIAVEFARLYAVELEHYEKLEGVPLTFAGKVNRLAIMVRGNLGAAMQGLVALP
                     LLVGYDIADPNPEAAGRIVSFDAAGGWNVEDEGYQSVGSGSVFARSAIKKLYSQVVDA
                     DSALRVAVESLYDAADDDSATGGPDLVRGIFPTAVTISAEGAVEVPEERIAELAREII
                     ENRTRTNTLHPDFTEPTDARGGP"
     gene            complement(21293..21487)
                     /locus_tag="VF874_20690"
     CDS             complement(21293..21487)
                     /locus_tag="VF874_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX9178386.1"
                     /translation="MAQEQTKRGGGGGDDDDLTGNAAAGQERRDKLTEETDDLLDEID
                     DVLEENAEDFVRAYVQKGGQ"
     gene            complement(21574..23085)
                     /gene="dop"
                     /locus_tag="VF874_20695"
     CDS             complement(21574..23085)
                     /gene="dop"
                     /locus_tag="VF874_20695"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEX9178387.1"
                     /translation="MQRIMGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAK
                     RTRWDYEVESPLRDARGFDLSRSAGPPPIVDADEVGAANMILTNGARLYVDHAHPEYS
                     APECTDPLDAVVWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKGASYGSHENYL
                     MSRQTPFSAVIAGLTPFLVSRQVVVGSGRVGIGPAGDEPGFQLSQRADYIEVEVGLET
                     TLKRGIINTRDEPHADADKYRRLHVIIGDANLAETSTYLKLGATALVLDLIEEGPAHG
                     LDLSDLALARPVHAVHVVSRDPTLRATVALADGRELTALALQRIYLDRVAKLVDSRDR
                     DPRAADVVQTWANVLDLLERDPMECADLLDWPAKLRLLDGFRHRENLSWSAPRLHLVD
                     LQYSDVRLDKGLYNRLVARGSMKRLVTEQQVLDAVENPPTDTRAYFRGECLRRFGADI
                     AAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLESAQTAAELVDQLTTT"
     gene            23138..24271
                     /locus_tag="VF874_20700"
     CDS             23138..24271
                     /locus_tag="VF874_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178388.1"
                     /translation="MPTPASTRRAAERWFLANGLPSVLPGRARWRGLWPRSAPVLAGY
                     AALMIVGMVIYLLVGTINVDIDREPTTAEWIVLGVLAACIPFVVLTGWLVARLRSYRA
                     RVAASTVAAVVAVVCDSINDGGTYVATTALGLLIVPLLTATGIGSVLGWALRLAVSQF
                     AAVGQLMLRALPVVLLTVLVFFNSPVWLMAASVTRARLWLALLFLGSIAAVFLVTSTL
                     ERIRPMLVAPNPFETHTHELAGTPFESMNDPATAEPLTLRERFNVVFVVIVSQLAQVL
                     TVAVVTALIFFVFGLILLNPELLAAWTRNPMSDGRLLWMTVPVPNALIQVTLFLGALT
                     FMYLSARAVSDGEYRSRFVDPLIDDLKLTLVARSRYRAATAGE"
     gene            complement(24244..24519)
                     /locus_tag="VF874_20705"
     CDS             complement(24244..24519)
                     /locus_tag="VF874_20705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178389.1"
                     /translation="MQQVQQRWRGREHSRVSGDDDNVYRGRAIVRDVGEQQQHRHQRA
                     DATDHRRRALDQRPGADPTGQHQRQPALQEPLVHRLEVRHSPAVAAR"
     gene            25031..25426
                     /locus_tag="VF874_20710"
     CDS             25031..25426
                     /locus_tag="VF874_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178390.1"
                     /translation="MSRPEKVNVLAVMVVSQAIQVVLFTAVIFAFFLTLGIIAVPDDV
                     AVVWSQETTCPDGAQPPCAGTWFGIKTPLTQTLVQTSLFVAVLSGLYFTVNSSVDPQY
                     RKRFFEPLIADLAVSLAGRDVYRSLDKAH"
     gene            complement(25406..26498)
                     /locus_tag="VF874_20715"
                     /pseudo
     CDS             complement(25406..26498)
                     /locus_tag="VF874_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557664.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
BASE COUNT         4220 a         9167 c         8876 g         4399 t
ORIGIN      
        1 ccccccgcga tcagttctgg cacgacggcg tcgatctgcg cggccgccag gtgcagcaca
       61 agtgtcgcgc cggacaacga cagcgtggcg aggtcctcgc ccggcggcat cgccgtcgac
      121 agcgtcgcca cccggctgag cgtgacggtt tgcgccaccc cgggcaccgt cagctcacgg
      181 cgtagcaccg cggcagcggc tgcgaaagcc ggtacgccag gcacgatttc gtagtcgatg
      241 ccgagcgtct ccaggcggcg ccactgttcg gctaccgcac tgtacagtga cgggtccccc
      301 gagtgcagac gggcgacgtc gttgccggcg gagtccgcgg ccgacagttc ggcgacgatc
      361 tggtccagcg tcagcagacc ggtatcgacg acgcgggcat cgggcgggca cagcaccagc
      421 aggtcgtcgg gcatgatcga cccggcgtag aggcacaccg ggcagcgctg cagcagccgc
      481 tggccgcgca cggtgatcag gtccgcggcg cccggcccgg ctccgatgaa gtagacggtc
      541 acctcttggt caccgcccac tgcgtgacgg gcatcgcggg gcgccagccg gtgaatttgc
      601 ccaacggctc gccggcgtag tgctggaagc gacgcagttc gccgcctagc gacgaatacc
      661 attgtgccag aatggcttcc gattcggcgg tgacggcgtt ggccaccagg cggccgtcgt
      721 cggggagccg ctcaaagcag gtttccagca gccccggctg cgtcatgccg ccgccgacga
      781 agatcaccga cgggtcgggt gcgccgtcga acgcgtccgg cgcggcgccg cggacctcga
      841 cactgacgcc gaacgcgacg acgttgcgcc ggatccggtc acgtcgcccg tcgtcgcgct
      901 cgaatgcgac ggccgtgcaa tcggtcccgg tgcgcgtcca ctcgaccgcg atgctgcccg
      961 agcccgcacc cacatcccac aacacctcgc ccggccgcgc agccagcgcc gccagcgtca
     1021 cggcacggac accgtgcttg gtgatctgcc cgtcgtgctc gaacacctcg tcaggcaccg
     1081 acgccacccg tgagtccggg agatattgca ctgcaacgac attcagatca tcgatgtccg
     1141 cggctggact gaccacccag tcgcgtgcgg tcgcgtcgcg gcggcgctcc gccgccccgc
     1201 cgagctgttc gaggatgctg acccgcgagt cgccgcggcc cgtgtcgacg agcagccgcg
     1261 ccagggacgc cggcgtcttt gcgtcgcgcg acagcaccac cgcgcgcccg ccgcggcgca
     1321 cggcggtgtg cggctcggcg gtcaccaggc tgatcacctc cgtgtcctgc acgctccagc
     1381 ccatccgcgc gcatgccagt gtcacgctgg agacgtgcgg cagcacgcgc acccgctccg
     1441 gcccgaacag ccgcaccagc gtgccgccga ccccgtgcag cagcgggtcg ccgctggcga
     1501 gcacatgcgc gtcgtccggc agcgcttgca gcaccggcat catcggcgac gaccactcgc
     1561 ggcgctgcgc cgcaaccgag tcgtcgagca ggtccagctg ccgacgggag ccgtaaatcg
     1621 tccgggcgcg gcgcagttcg gcacgggcct gcgccgacag gccggccgtc ccgtccgcgc
     1681 cgatgccgat cacgacgatc atggtgacga ccctaacgcc ggtgaggata gcgaccgctc
     1741 tcaagctgcc ggactgctgc gtcctctaag cggcggaaca gcacggcgcc tgggcctggc
     1801 cgtcgcgtaa tcacctcggc agccgccgcc acacccaccg cggcagcagc cgcatcccga
     1861 agaacactgg ccgcagcacc cacggcaccc acacctcgcg gcgacctttc gccagcgcgc
     1921 gggccgtcgc cgcggcgacc tgctgcggtg tgctggagaa cggcgccggc gtcataccct
     1981 gcgtcatccg cccgatcacg aacccgggcc gcaccagcaa cagctgtaca cctgagccgt
     2041 ccagcgcgtc ggccagcccg ctggcgaatc cgtcgagccc cgctttggcc gacccgtaga
     2101 cgtagttcgc gcggcgcacc cgcacaccgg cgaccgacga gaacaccacc agcctgccgc
     2161 ggcccgccgc tcgcatcgcg tttgccagat gcgtcaacag gctcacctgc gcgacatagt
     2221 cggtgcgcac gatctggacc gcgtgcgcgg cgtcgtcctc ggcccggctc tggtcaccca
     2281 gaatcccgaa cgcgagcacc gcggtggcga tcgggccgtg ttcggcgaca agcgaatcca
     2341 ccaacgggcc gtgggttgcg aggtcgtcgg cgtcaaaggc ggtcacgtgc acggctttgg
     2401 caccggcagc ctgaagcgcc tcgcgctgtg agtcgagacg ctgcgggccg cgcgcggcga
     2461 gcaccaccgt ggcgccggca gcgagccgga ccgcgagctc ggtgccgatc tcgctgcggc
     2521 ccccgaagat cacgaccacg tccgtgtcgc ccacgcctgg accctatcgc ctgcgctagc
     2581 gtggtcggcg atggcaaact cggcgaccac gcgattgacc accgaagcgc tggcgttcct
     2641 gaccgagcgc catctcgcca tgttgaccac actgcgcgcg gacaactccc cgcacgtggt
     2701 ggcggtcggg tttacgttcg acccgaagac gcacatcgcc cgcgtcatca cctccggcgg
     2761 gtcacagaag gcggtgaacg cccagcgcgg cggggtcgcg gtgctgagcc aggtcgacgg
     2821 ggcgcgctgg ctgtcgctgg agggaaaagc gagcgtgaac accgatccgg aggcggtccg
     2881 cgacgccgaa ctgcgctacg cgcagcgtta ccgaacgccc cgggcaaatc ccaagcgcgt
     2941 cgtcatcgag gtgaaggtcg agcgagtgct gggatcgtcg gagctgctcg accgctcata
     3001 gctgtcagac cggcacgacg gtgagttcgt gtggccggtg gttgaccggc tctgctccat
     3061 aggacgtcac gatcacgatg tcttcgatgc gtgcgcccca ccgtccgggg aagtaaatgc
     3121 cgggctcgac gctgaacgcc atgcccggtc gcaacggcag ccggtttccg gccacgatgt
     3181 aaggctcctc gtgaaccgac aacccgatgc cgtgcccggt gcgatgcacg aagttgtcgg
     3241 cgaggccggc ctggcccagc acgccgcgcg cgacagcatc cacctgttcg gcggtgacac
     3301 ccgggcgcac ggccgcgaca gccgcctgtt gcgcgcgctg gagaaccgaa tagtgttgcg
     3361 ccacttctag atttggctcg ccgatgctgt aggtgcgagt ggagtcggag tggtagccgg
     3421 gttcgtaggt accgccgatg tcgacgacca cgatgtcgcc aggctgcaac acgcggtccg
     3481 agcattcgtg gtgcgggtcg gcgccgtgcg gaccggagcc gacgattacg aatgccacgt
     3541 ccgaatggcc ctcggccaca atcgctttgg cgatatccgc ggcgacgtcg gcctcggtgc
     3601 ggccgggcac caggaattcc ggcacccgcg cgtgcacccg gtcgattgcc gcgccggcct
     3661 tgcgcagcgc gtcgatctcg ctgtggtcct tgatcatccg cagctgacga agaacatccg
     3721 tagccagcac cggcgtgaca ccgagcagct tggcgatcgg cagcaggtga agcgccggca
     3781 tcgaatcggt caccgccgtc gccacaccag cgccaccgag cgcgtcgacc accagcgagt
     3841 agggatcctg cccgtcgacc caatcgcgca cccccagccc aagttcggtc accgccgagt
     3901 ccttcagcga cgccaactcg agccgcggca caaccacagt cggggtgccg gcggccggca
     3961 gcaccaacgc ggtgagccgc tcgaacgtct gcgcctgagc gccgaccaga taccgcaggt
     4021 catagcccgg cgtgacgatc agtccggcca gtcccgccgc ggtagtggct ttggcagcct
     4081 cgtgcagccg tcgggcatag gcgtcggagc tgaatctgct gaaagccatg ccggaaaggc
     4141 taacgccccg cgtcctccaa ctccgtcaac gacaagtcaa gtccgtcgag cagcgtccac
     4201 acgtccggaa cctgctcgcg gtcggcgacg atgccgatgt ccacctgacc gtcgtagctc
     4261 atcacggtga tgttgaggcc cagcccgtcc gcgatcgcag acaccggata gttggccacc
     4321 aactgcgcgc cgccgcaata gagcgggaac tgcggccccg gaatgttcga caccacgacg
     4381 ttccatgtcg ggcggcccac cgacgaagtg gccagggcga acgtggcccg tgccgcacgg
     4441 ttgaacagcg ccggcggaat gaaatggttg atgtcgcaga gcagatcggc cggcagcgca
     4501 cggtggcgtt ccttcatttc cccgagcccg gccgctgtcg ccttgagccg cgccgccgga
     4561 tcggcgatgt cggtgtgcag cggcgccgac atcatcagca cgtggttgcc gtaggtgccg
     4621 gcctgctcct cggtgcgcac cgacaccggg atctgtgcga ccagcggctg ttcgggcagt
     4681 tccttgtggt cgagcagcca gcgccgcaca gcaccggcgc agatcgtcac cacgacgtcg
     4741 ttgaccgtca caccgaaggc gttcttgacg tctttgacgc gttgcaactc cagccgcccg
     4801 aacgcaaacc gccggttggg cgacacccgc cggttgaacg acgtcttggg cgccggcagc
     4861 ggcgaccgga tgaccggatg gctgtcgggc cgggcggccc gttgcagccg acccagcagc
     4921 cggcccgtct ggctcactcc cggcactgcc gacaacgtcg tctcctcgag attgggcagc
     4981 gcgtgcggca acgacctgag cacccgaatc ggatagcgct ggaccccgaa cagcgcgcgg
     5041 ccccacatct gcaaacttcc cggctgccgc tcgggcgtcg taggctcggc cggcggaatc
     5101 ggccggcccc gcggactgac atccagcagc aagccggtga tctcagcgcc cgacacgccg
     5161 tcgatcagcg cgtgatggat cttggtgtac accgcgacgt agccgttgcg caggccgctg
     5221 atcacataca tctcccacag cggacgggcg cggtccagct gccgggcatg cagccgcgag
     5281 acaagttcgg ccagctgatg gtcgccgccc ggggatggca acgccgactc acggacgtgg
     5341 taggtcatgt caaagtcgtc gtcgacgacc cagtacgggt agtccagccc gaacggcacc
     5401 tcggccagcc gccatcgcag tggcggcagc aagtgcatcc gctcgttcag caggtccatc
     5461 atcgtcgcgc aagtgagcgt cccgttcggt gcggtgctcg ggtcgtggat cgccaacgcg
     5521 gcgacgtgac cggtccggcg cccgtcttca atcgcgagga actgtgcgtc tagactgctc
     5581 agctgcttca tgtcggtgac gcctctcgtt cggtttcggc gttctccacg gcgcgcgcga
     5641 cgtcgcggtt gtgcagcagc acgtcgtcca tcacagcggt cgtgaagctg cgcatggcca
     5701 gcatgggcgc cacccacgcc ggagcggtga cccgcgcggc ccgccgcgaa atgccgtcga
     5761 gcaccgcctt ggccgcgtcg ctcggcggga tcggccgtgt caggaagccg ggcgccgcgt
     5821 cgcggatcgc ggcggcgccg tgctgcgcga aggcgtcggc caccaggtcg gtgtcgatga
     5881 agcccaggta ggcgaagccc gccgtgacgc cgtgcggcgc gagttcgacg cgcagcgccc
     5941 gggccagctg ctcgacaccg gccttgctca tggcataggg cgcggcgcgc acgccgttga
     6001 agaacgcgta gatcgagccg atgaacatga tgtgtccccg ccgctcgatc agcgccggca
     6061 gcgtggtccg cgcggtccgc cactggccga gcagatccac ttcgacggtc cgctcgaagt
     6121 cggcggggtc gatggtggcg acgggctgcg acgacgaggc gatgccggcg ttggcgacga
     6181 ccacgtcgat ggcgccgaac gcgttgacgg cgccgtcggc cgcgcgctgc agcttgtccg
     6241 cgtcgatgac gtcggcttcg aacgcagcgg cccgctcgcc cagggtgtcg gcgaggttct
     6301 gcagcggctg caggtgacgg ccgaccagcg ccacgtgcgc gccgctcgca tgggcctgcc
     6361 gcgcgatttc ggcgccgagc ccgcgcgcgg cgccggtgat gaacaggacc cgcccgccga
     6421 tgtccaactc cggccggcgc ccgaacagcc cactgaccgc cggcagcgac tgcccgagcc
     6481 gagaggtggt gcgcagcagc aggcgggcga cgtcatcgat catgctcacc tgagttgccg
     6541 cctttccgcg ctcctaacct gccgcccgga tcggcccggg gattcgacgt aatcgcaggg
     6601 ctaacatatc gagcgcacgc aatgtcgcgg gggctgttca gagagagaag ccaccatgga
     6661 ttggtccacg gccgcagttg tctctgctgt gatcatcgcc ggcttggtcg cggtgaccga
     6721 ccgcttgagc tctcgcttcg gcaaggactg agtacgccac tggcatgatg accgcatgcc
     6781 ggctcccctg ctgttgctcg acggagcgag catgtggttt cgctcgtact acggtgtgcc
     6841 ggcgtcaatc acctcgcccg acggccgccc ggtcaacgcg ctgcgcgggt tcctggacgc
     6901 ggtggcgacg ctcatcaccc gcgagcgtcc ggcccggctg gtggtgtgcg acgactggga
     6961 ctggcggcct cagtggcggg tggaccggat cacgtcctac aaggcgcatc gcgtcgccga
     7021 gccgaacccg gacggcgaac ccgacgtcga agaggtcccc gacgagctga caccgcaagt
     7081 cgactggatc atcgaggttc tcgacgcgtt cggcattccc gtcgccggcg cacccggcta
     7141 cgaggccgac gacgtgctgg gcacgctggc ggcccgcgag cggcgcgatc cggtggtcgt
     7201 cgtcagcgga gaccgcgacc ttctgcaggt ggttaccgac gagcccgtcc cggtcaaggt
     7261 gttgtacctg ggccgcgggc tgtccaatgc gacgaagttc ggcccggccg aggtcgccaa
     7321 ccgctacggc gttccggcgg atcgcgcggg accggcgtac gccgagctgg cgctgctgcg
     7381 cggcgacccg tccgacgggc tgcccggcgt cgccggcatc ggggagaaat cggcgacgac
     7441 gctgctttct cagtacggct cgctggcggc catcctcacg gcagccgatg atcctaaatc
     7501 gaagctgtcc aaggcgtttc gcagcaagct gctcgccgcc cgcgactacc tggacgccgc
     7561 cggcccggtg gtgcgggtgg ccaccgacgc tccggtgaaa ctgtccacga agacagatgc
     7621 cctcccgctg gcggccgcgc atccgcgaaa agtcgccaag ctggccgagc aatacggcgt
     7681 cggctcgtcg atcggccggc tgcaaaaggc actcgacgcg ctgccggcga ggtgactact
     7741 tgggccggcc gacctcgtag gtgccgttgt tgtcctggaa cgtcacggtc acctgcttgt
     7801 gcgtgccgtc gacgctggcg tcgcaggtga acgtgtcgcc cttcttcacg gtggggttct
     7861 tgccgccgtt gcacttgacg tcactgacgt tgtcgacgcc gtaaccggtg gtctggttcg
     7921 tcaagatgtc cttgacgccc tgttgcgcct tgttcacgtc cagcttcgtc gtgacgaaga
     7981 atcccggctt ccagaagccc atgaccaaga cgacggcgac gatgatcgcc gcgagcacac
     8041 cgatgacggc gccgagaacc gccagcgacg acttcgagcc cttctcggct ccggcgtccc
     8101 cgaacgccgc cggagcttcg ccgtactgcg cgccggcgcc cgggtactgc cccggctgcc
     8161 cgtacccctc ggtggccgga tactgctggc cgtactgagg agcctgcggg gcttgatact
     8221 gctgcggctg gccgtactgc gttggctgcg ccgccgcgcc ctgctcggtt ggtgaatacg
     8281 gctgctggcc gtattgcgtt ggctgcgccg ccgcgccctg ctcggttggt gaatacggct
     8341 ggccctgcgg gtactgctgc ggggaatacg cgggcggctg ccagccggcg tgctgcgtcg
     8401 gctccgacgg actcggctcc tgccccggct ggcccgatga ccattgctga ttgggatcgt
     8461 aaggtccgct catcgtgctt ccgttctcct cgtcatggca gctatccttg ctcttcgcgc
     8521 gagcgctcat cggccatctt tgctcttcgc gcaagcgctc atcggccacc gtagcgttct
     8581 ctcagcctac ccgccatcaa ccgcgacgac gccgcgccga atctcctcga tcgcgcgttt
     8641 cgcggaagta cgtaggccct tgtcggcggc cgcgatacga atctggtcga gcaggtcgag
     8701 cacttggcgg caccaccgca cgaaatcgcc ggccggctgg gggtacctcc cgcttgcggg
     8761 ggacggagtt cccgtcccgg cgacatcgga ggcttccagc gccgcggtca ggtcgccggt
     8821 ggtggcccag cggtacaccg cggtaacgaa cccgtcgtcg ggctcgcggc tggcgtccaa
     8881 gcggtggcgc tgttcgtcgc cgcgaagtgt gacggccagt cgccgggtgt ctgccagcgc
     8941 gcggcgcagc gctgcggtca ggatctcccc cgcgcgcatc ggcgccggtc cgtcggcccc
     9001 gcgggactcg tagaccaccg ccgacacgac ccccgccaac tcggccgagt tcaggccgcg
     9061 ccacactccg ccgcgcaggc actcggcgac caatagatcg ctctccgaat agatccgggc
     9121 caacagccgt ccgtcgtcgg tgacccgcaa cacgccattt ggccgctggt ggatgaagcc
     9181 gcgctcggtg agcagtccga cgatacggtc gaacgtgcgc gctaacgagt tggtggccgc
     9241 cgcgaccttg ccgcgcagct gctcgttctc gcgctcgagc cgcagatacc gctcggcgac
     9301 acggactttg gcttcccggt cgccgagtcg gtgcacaggg tgatgacgaa tctcgtcgcg
     9361 cagcggccgc agttccgggt cctcgcccgg ctcggtgcgg cgggtcctgc ggctgggtgt
     9421 cgtcagcccg gcggccgccg atcgcagcgc cgaagcgaca tcacggcgca cccgcggctg
     9481 gcggtgctcg acgcgcttcg gcagcgtcat cgaccccagc ggtgccgcgg cgtcggagca
     9541 gtccgccgac gaaatccttc cggcccaacg gttttcggtc agcaccagcg gccgcggatc
     9601 cgtcgggtca cggtcggctt ccaacaccac cgccagcccg ccgcgtcggc cgtgcgtgat
     9661 cgagatgatg tcaccgcgac gcagcgacga cagcgcctcg ttggcagcct ggtggcgctg
     9721 cagtcgcgac gaacgtgctt gtacccgttc ccgttcactg agtttcgcgc gcagccgcgc
     9781 gtactcgagc acagggtcgt cgccgagttc gtcggcaagc tccctcatca acttctcgtt
     9841 gcgctcgatc gcgcggacca gcccgaccac cgaccggtcc gcctgatact gcgcaaacga
     9901 gcgctccagc agctcgtggg cctgatgcgg tcccatctgt gaaaccaggt tgatcgtcat
     9961 gttgtatgac ggcaaaaagg aactgcgcag cggaaacgtc cgagtggagg ccagcccagc
    10021 cacctcggcg ggatccacgt ccggctgcca cagcaccacg gcgtggccct cgatgtcgat
    10081 gccccgccgg cccgccctgc cggtcagctg ggtgtactcg ccgggtgtca acggaagatg
    10141 ctgttcgccg ttgaacttca cgagccgctc caacaccacg gtgcgagccg gcatgttgat
    10201 gcccaacgcc aaggtctcgg tcgcgaacac gaccttgatc aatccggcgg tgaacagctc
    10261 ctcgacggtg tggcggaaaa tcggcagcat tccggcgtgg tgcgcagcga tgccccgcac
    10321 cagtccttcc cgccactcgt ggtagccgag gacgacgagg tcggcatcgg acagatccgc
    10381 agtccgccgg tcgatcacct cgatgatgcg gccgcgctcc tcgtcgtcgg tcaaccgaag
    10441 cgacgaccgc aggcactggc ccaccgccgc gtcgcacccc gcgcgggaga acacgaacac
    10501 gatcgccggc agcaacgcgc tgtggtcgag caccgaaatc acgtcgggac gcgacgccgt
    10561 ccgatacagg cgcgggcgcg gaccgcggcc acgacctcgc ggcgctgcgt tgagcagccg
    10621 gtcggcctcc cggcggttgg cgatgtgctg caacagcatc gggttgacga gggcgccgcg
    10681 tccggcgttg tcgggcctgt ctccgccgga gtagtcgaac aggtcgaaca gccgcttgcc
    10741 caccatcatg tgctgccaca gcggtaccgg ccggtgttcg tcgacgacga cggtggtgtc
    10801 cccgcgcacg gtctggatcc agccgccgaa ctcctcggcg ttggagacgg tcgccgacag
    10861 gctcaccagg cgcacctcgt cgggcaggtg caggatgact tcttcccaca ccgccccgcg
    10921 catccggtcg gccagaaagt gcacctcgtc catcactaca tacgaaagac cttgcagcgc
    10981 gggcgaatcc gcgtacagca tgttgcgcag cacttcggtg gtcatcacca ccaccggcgc
    11041 gccggaattg atggacacgt cgccggtgag cagaccgatc ttctccgggc cgtagcggcg
    11101 caccagatcg gtgtgcttct ggttgctcag cgccttgatc ggggtggtgt agaagcattt
    11161 ccggccggct tccagcgcga ggtgcacggc gaactccccc accaccgtct tgcctgcgcc
    11221 ggtgggcgcg cacaccagca ccccgtggcc gccttccagc gccgagcacg cctgtacttg
    11281 gaacgggtcg agcgtgaacg gcaacagcgc agagaacttc gccaactctg aagtggcgct
    11341 cgtcgtcatg tcaccttcga gggtggctcg atcgcagagg cctcgtcgtc ggcgaccgcc
    11401 cagccctcgg ccgcctcccg tttcgccttg cgcttgtcgt gtacccgcgc gatctgtatt
    11461 gcaagctcga gcaggatggt cagcgccaag cccagcgcga gcatcgaaaa gggatcggat
    11521 cccggcgtcg cgaaggccgc gaacacgaac accccgaaga tcagcccgcg ccgccacgcc
    11581 ttcagccgcg tgtaagtcag cacgccgatc aagttgagca tgatgatcag cagcgggaac
    11641 tcgaagccca ccccgaacac cagcagaagg ttgatcaaga agccgaaata ccggtcgccg
    11701 gacaacgcgg tcacctggac gtcgctgccg acggtgagca ggaagtgcag cgccttggac
    11761 agcacgatgt aggccagcac cgcgccggag acgaaaagca cagccgcgca gatcacgaaa
    11821 cccaccgcga accgccgctc gttcttgtac aggcccggcg tgatgaacgc ccacaactcg
    11881 tagagccaca ccgggcacgc cagcacgatt ccggctgcga ggccgacctt cagccgcagc
    11941 atgaactggt cgaacggcgc ggtcgcgagc aggcgacagc caccgtcggc gctgatgtca
    12001 gcccgcgcgg acggcggcag cgcacagtac gggtgccgca gccactcgcc gaggctctct
    12061 accccgaaga tcccgtggct gtaccacacg aagccgacga tcgtggtgag cgcgacggcc
    12121 cccaccgaga tcaacagtcg gtaacgcagc tcgcggatgt gatcgacgag cgacatcgtc
    12181 ccgccagggt tgaccctact tcgacggcgg cgtgggtcga ggcggcgcag gaaaccaggg
    12241 gtcttcacgg gtggttgtca gtggcgccgc attgtgcggc gagcggtgtc gacggggcgc
    12301 gcctcaggcc ggcctggcgt cggtgtggtt ctcttcggga gccggtgtgt cgacgcgctc
    12361 cgactgcacg gggttaatct tcggcggatc ttctgaattg ccctcatgct tcaactcgcg
    12421 catctcggac ttgaagatcc gcatcgattt cccaagcgag cgggcggcgt cgggcagctt
    12481 cttggaaccg aacacaacaa tgaccaccac gacaaagacg atcacgtgcc agggttgtag
    12541 ggcgttcacc atggtttgcc tccagacgtc gcctcgatgc taccgcagcg agtccgcctc
    12601 atacgcctgc agcgccgccg tcgccgcact ctgcacctgc gccgccagcg acgacggggc
    12661 gagcaccttc acggccgcgc cgaagcccag caccagccgc gccatccact cctccgacgc
    12721 gtacgtcatg tccgcttcac aggacccgtc cggcagttcg cgcaccatcc gcatcgggaa
    12781 gtagtcgaac atccagctcg cggtcggcgc gatgtgcagc gtggccgacg gcagcgacgg
    12841 gtcggcgtcg aacagcgacg tgtccggccc ggcttcgacg gccagcggcg gcggcgccga
    12901 cggctcgtcg agcacctgcg ccgagtcgat gcggtcgaac ctgaaaaggc gcacgccctc
    12961 ggcgctgcgg caccacgcct ccaggtagct ctggtcgccg acgaacagga tccggatggg
    13021 gtcgacgacc cggtgcgacg acgtatcgtg cgacgccgag tagtagtcga tcgccacggc
    13081 gtggtgcagc cggaccgcct cgcggacggc ggcggccgcc gggctttcct cgctggcgtc
    13141 gtcgggcatc gccccgccct gcggctgccc cgcggcggat tcgatcttgg cgatcgcgct
    13201 gcgcgcggcc tgcgggtcga ccatgcccgg cacttcctga agtgcccgca gcgcgaccag
    13261 catcgccgtc gcctccggcg acgtcagccg cagcggccgg tccatcccgg ccgagaagat
    13321 cacctcgatg ctctcctcgg agaacgacag gtcgatcaga tcgcccgggc tgtatccggg
    13381 cagcccgcac atccacagct ggttcaggtc ggtcatcagc tgctcggtgc tcaccccgag
    13441 gtccgcggcg gcctcgacgg cgctgattcc agggttggcc tggaagtacg gcaccatgtt
    13501 cagcagccgc accagccgcg tcgacagttg actcacgcgc tcaccgcctg agcccgcagt
    13561 cgcgcgatga cctcgtcgcg cagcgactcg ggctccagca ccacggcgtc ggccccgtac
    13621 ccggccacct cgcgggccag ccggtcgacc gagccgatgt cgatcgccac gacgtcgccg
    13681 tcgcggccgc gcagcgtcat ggactcgacc actgcgccgg cccgccgcag cgcggtggcg
    13741 cggtcgtcgg ccacccagat tcgcgcttcc acgccggtcg gtgcttcgcg gacggctttc
    13801 gcgacgatgt cgcgcaggtc gacgtcgtcg ggcttgcgca ccgctcccac cggcccgatc
    13861 ggcgtcactt ccgcgccgat gcgcgacaac cggaacgtgc gcaccgcgtc gcggtcgcgg
    13921 tcctttccga ccagatacca gcggccgcgg tcggtgacca ccccccacgg ctcgacggtg
    13981 cgaatactgt acggctcgac gcgcgacgac cgatgctgaa attgaaccgc ctgcccagag
    14041 ccgatggcgc tcaacaggtt tggcaacacc ggttcggagc cgcgaatgcc gggcagcgcc
    14101 gatgtcgacg cgatcgacac gctgatgtcg ggcgcgtcca cgtccacgcc ggcggcgcgc
    14161 agcttcagta ccgcgccctg ggtggcggtg atgagttccg gtgactgcca cagctgcacg
    14221 gcgacggcga cggctgccgc ctcgtcggcg gtcagcgcga tgtcaggcag cgcgtacgca
    14281 tcgcgcttga tccggtaccc ctcggcgggg tcgagcgtgc tgacgcggcc ggtctccaac
    14341 gggatgccca gatcgcggag ctcgttcttg tcgcgctcga acattcgggc aaacgcgtcg
    14401 tcggtcgggc agtcggcgta gcccagaacg gtcttgcgaa ttcgttcggc ggtgatgaag
    14461 ttgcgggtag acagcagggc gatgacgaga ttcatcaacc gctcgacttt ggagatcccc
    14521 acccgcttag cctaattgcg ccgaaacggt attccacgtg gtccttactc gcgttttccc
    14581 gcggcagtta ccgcgtcggc ggctcacatg ctggcgatca gccgcttgac ccgctcgtcg
    14641 actgagcgga acgggtcctt gcacagcacc gtgcgctgcg cctggtcgtt gagtttgagg
    14701 tgcacccagt cgaccgtgaa gtcgcggccc gcttcctgcg cggcgctgat gaactccccg
    14761 cgcagcttcg cccgggtggt ctgcggaggc gtgtcgacgg cctcctcgat ctcctcgtcg
    14821 gtggtgaccc gcgtcgccag gcccttgcgc tgcagcaggt cgaacacgcc gcggccgcgc
    14881 ttgatgtcgt ggtaggccag gtccagctgg gcgatcttgg gatcggacag ctccaggttg
    14941 tagcggtctt ggtagcgctg gaacagcttg cgcttgatca cccagtcgat ctcggtgtcc
    15001 accttggcga agtcctggct ttccaccgcg tcgagctggc ggccccacag gtcgacgacc
    15061 tgctcgatct gctcgttggg ttcccgggtc tgcaggtgct ccacggcgcg ggcgtggtac
    15121 tcgcgctgga tgtccagggc gctggcctgc cgaccgccgg ccaggcgcac cggacggcgg
    15181 ccggtgaggt catggctgac ttcgcggatc gcccggatcg ggttgtccag cgagaagtcg
    15241 cggaacgcca ccccggattc gatcatctcc aacaccagcg cggcggtgcc gaccttgagc
    15301 atcgtcgtgg tctcgcacat gttcgagtcg ccgacgatca cgtgcagccg ccggtatttc
    15361 tcggcgtcgg cgtgcggttc gtcgcgggta ttgatgatcg gccgggaccg cgtcgtcgcg
    15421 ctggagacgc cctcccagat gtgctcagcg cgctgggaca ggcagaacgt cgccgccttc
    15481 ggcgtctgaa gcaccttgcc cgccccgcag atcagttgtc gggtgaccag gaacggcagc
    15541 agcacgtcgg agatccggga gaactccccc gcgcgcacga tcaggtagtt ctcgtggcag
    15601 ccgtaggagt tgccggcgga gtcggtgttg ttcttgaaca aatagatgtc gccgccgatg
    15661 ccctcgtcgg ccagccgctg ctcggcgtcg atcaagaggt cctcgagcac gcgttcgccc
    15721 gcgcggtcgt gggtgaccag ctgtgccagg ctgtcgcact cggcagtcgc gtactcggga
    15781 tggctgccga cgtcgaggta cagccgcgcg ccgttgcgca ggaacacgtt gctgctgcga
    15841 ccccacgaca ccacgcggcg gaacaggtag cgcgcgacct cgtcggggct gagccgacgg
    15901 tggccgtgga acgtgcaggt gacaccgaat tccgtctcga tgcccatgat tcgacgctgc
    15961 acgtaatcga gcgtacttgt tacgcggaca agctgacgtg taacccgccc ggcgacccgc
    16021 gagccactac gccccggcct ggattccctg gttgacctga tcgatggcct gctcgaccaa
    16081 cgagatcgcc gccactcggt ggccggcctt gtcggggatc gcctgcagct cactttgtgc
    16141 ctgctgcact gcctcgtcgc ttcggcgtcg gaggtgtgcc gggggtcgac ctcaattaac
    16201 gtgcgcccag cctcttccgc tttggcgcgc agtatcgaga cgaaccggcc ccagccggcg
    16261 tcactgatgc ttctagaaag cccggacttc gcccgcgcca ttggccagat actggccggg
    16321 attgtcgggg ttcgccgccc cgccgcagct ggatctgtac gcccgcaccc cctactacca
    16381 gggcacggaa acgttcgcac acgtaccgtt cggccgcctc gacgcctgat ctgccctgtg
    16441 gataacgcga gcggagggct gcgcgcagat gccagcatga ggcatggggg gaagaccgac
    16501 gctctctcag gcgaaatcgt ggcatccgga ctcgttgcgg caggcggccg acgcctggga
    16561 tgcggccgcg atggatatcc gtcgagagtt cgacgtggtg gtgcaaggca tgcacgacac
    16621 gcacgacttc tgggtcgggg acgctgctga ggccgcgcgg gagcgggcgg ccagaacggg
    16681 cgccgacgca gccagactcg cccgcgccct ggtggccgcg gccgtggccg cgcgcgacgg
    16741 cgccgacgtg attgcggctg cccgagacga tgtgctcggc accgtcgccg cggtgcagtc
    16801 tcagggcttc gcggtgaccg acgacgggac cgtcacaccc cccgcgcagt cacccccggc
    16861 cgtgcttgcg acggccgggg gcaacatgca gatcgctcag gaggtattgc gttccggcgc
    16921 cgaggaattc acccgcgaga tcgtcgagaa gctcgacgcg ttgggcgccg cggacgacga
    16981 cgccgcccgc gacatcgacg aggcgttcca cggggtgacg acgccgcaac ccactgcgcc
    17041 caccgggctg gacgacaacc cggtgggtaa ctggccggcc atgacccagg acagcattgc
    17101 ggcgcagatc gccgcgatga agcccgagca gcggcggcag ctgatcgaga gctacccaca
    17161 gcaggtcggc aacaccgacg gcgtcccgtg ggacatgcgc gtcgccgcca accgactcaa
    17221 catcgagcag gccattctgg acgggcgccg cgaactgaag cggacacagg accccacgca
    17281 gcgcgacgcg gtccagcagc gcatcggctt ctaccagggg ctgctgtcag aggtgcccga
    17341 tccgaccggc cggaaaggcc gggtcgatcg ccagatcctg gccttcgatc ccggccgctc
    17401 gtcgctggtc gaactgatcg gcgacctcgg caccgcgaac aacgtcggcg tcctggtgcc
    17461 tggcatgaac accaccatgg agggctccgc ggccaacact gagacggcga cgcggttcgt
    17521 gcaggccgca cacggtgacc tggcgatgat cacgtacctc ggcggcccgt tcccgcaagg
    17581 cggaatcccg gacggcatcg acgacgcgat ggacaccgac tatgccaccg gcatggcgcc
    17641 gcggctggtg tcgttcagcg aggacgtcaa ccgcgtcgtc gacgccacgg ggcgcgacat
    17701 cgcggtcacc tacgtcggcc actcctacgg cggatccgtt ctcggcaccg ccgaggcgat
    17761 gggcctgacc gccgaccgca cggtctacgt cgaagcggcg ggcgccggcg tcggagtcga
    17821 cgacctcggc gactggcaca acgagaatcc gtatgtgatg cggttttcga tgaccgcgcc
    17881 gggcgactgg atcgagccgt tgcagggcat gccgctcaac ccgcacggcg ccgaccccga
    17941 cgagatgccg ggcgtgatcc ggcttgccac cggcgactac gacgacggcc agcggatggt
    18001 cggctgggat acacacagcg acgtcctcaa cgaaccctcc gatgcgtggc gcaacattct
    18061 cgcggcgatg accgggcagc cgtacagcgt gtacgtcgac ccgctgaagt tcctgccgac
    18121 ttgggctaaa ccaccctggc tgcggctgac ggggtgaata attgggcggt tcccaggagg
    18181 ctgccgtgac aactacaggt acgccaatcg agaagtctgc gcgggtgcag agctgcgacg
    18241 tctgcatcgt gggagccggc atcgccggac tcaacgccct gttcgtcgcc agccgatacc
    18301 tctctcgtga tcagaaggtc atcctggtcg acagccggga acgcgtcggc ggcatgtggg
    18361 tcgacacata tccgtacgtt cgactgcacc agccacatcc catgttcacc gccggcaaca
    18421 tcaagtggac tctcggccag gatcgctcct accttgcgac caagggcgag gtgctcgacc
    18481 acttcgaaca ctgtctcaac gtcataaaac agcgggtgca ggttgacgag ttcttcggtt
    18541 ggacgctgga atccgacgat gaggccgacg ggatcgtgcg gataacctgc agatccgcgg
    18601 atggcgggct tctggtcgtc gaagcgaacc gactgatcaa ggcctacggc ctccgggtca
    18661 tgcccaacga cccgctcgaa atctccagtg cgcgtgtcca atccgtgtct cccgactact
    18721 gcgacgtccg ttcgggcgac atgcgggcga gcgatacccc ggtgtggatc atcggtggcg
    18781 ggaagaccgc gatggatacc gcgcatgcgc tgatcacgga ataccccggc cgtgacgtga
    18841 acctggtggc gggttcgggc acgttcttca caagccgcga ccgcttattc gctaccgggg
    18901 cccggcgttg gtggggcggc acgcttggca gcactatcgg cacggaaatg gccagacgtt
    18961 tcgacggaac caacgaggca gaggtcgagg agtggtatcg cgggacgtac gggacatggt
    19021 tgacccccag caccagcaac ttcgtcttgg gcgtcctgtc cgagtcggag aaacaaacga
    19081 ttgccgcagg gctgaacgac gtgatcatgg atcacctggt ggatgtcgtc gaccgcgacg
    19141 gcgcgacgga actgatgttg cgtagcggat ccacgaaagc gatccaaccg ggcagttgga
    19201 tagtgaactg cacgggatac ctcatggctc gcggccactc ctacgagcct tatgtttccg
    19261 acagcggggc ggtggtctcg atccagccgc gatcggccac gctccacttg ccggcgttca
    19321 tgggctattt ccttgcgcac ctgctgtttc tgggcaagct aagagacgtt ccgctgtatg
    19381 agattgacgt ggaagatctg aggacgaagt cgcgtgccgt gttcccgtac acagtctttg
    19441 cgctcgtgca gcacaacctc agcctcatct tcgacagtgt tccgagcaag gtgtttggcg
    19501 agtgcggact cgacttcgac acctggtacc cgttgccgcg ccgcttggcg agtacggcac
    19561 ggttcatgct cacgcaccgc cgggtacgcg agcgccagcg ccgcgtcctc gatacggtgc
    19621 gcgagcgatt cgatgttcgt tgcggcccct tgacccacga gtgagccgta ctcagtgcta
    19681 atccccttgt ggcagtagcg attccagtgc ggacccggtg atgcggcgaa acgcccggcg
    19741 cggccggttc tgatcgagga tggccacctc cagcgtcgac accccgagcg tgcgcggctc
    19801 ggacgaaccc gacccgttgc cgctcgtgcc tgcctgcaaa gccttgaccg caatcccgac
    19861 ggcctcggcc agcgtggcgt tctcttggta cgagtccttc agcgcggtgg tgatcggctc
    19921 ggtcaagccg cccatcacga cgaaatgcgg ctcgtcgttg atcgagccgt cgtaggtgat
    19981 ccggtacagc tcaggcggtt tggtctcgcc gtagtgggcg acctcggcga cgcatagctc
    20041 gacctcgtag ggtttggcct gctcggtgaa gatggtgccc agcgtctgcg cgtacacgtt
    20101 ggccagctgg cggcccgtca cgtcacggcg gtcgtaggcg tatccgcgcg tgtcggcgaa
    20161 ctggatgccg ccgcggcgta ggttgtcgaa ctcgttgaac cggccgaccg cggcgaaccc
    20221 gacgcggtcg tagagctcac tgaccttctg cagagagcgc gacggattct ccgcgacgaa
    20281 cagcacgccg ccggagtacg ccagcgcgag aacgctgcgg cccctggcga ttcccttgcg
    20341 cgcgagctcc gagcgctcgc gcatcgcctg ctcgggcgag atgaagtacg ggaaggtcac
    20401 gggccgcccc gcgcatcggt cggttcagtg aagtctggat gtaaagtatt tgtccgcgta
    20461 cggttttcga tgatctcgcg ggccagttcg gcgatgcgct cctcgggcac ctcgaccgcg
    20521 ccctcggcgc tgatcgtcac ggcggtcggg aagatgccgc gcaccaggtc ggggccaccg
    20581 gtggctgagt cgtcgtcggc ggcgtcgtag aggctttcca ccgccacccg cagcgccgaa
    20641 tccgcatcga ccacttggga atacagcttc ttgatcgccg accgagcgaa caccgacccc
    20701 gagcccaccg actggtagcc ctcgtcttcg acgttccagc cgccggcagc gtcgaacgac
    20761 acgatccggc cggccgcctc gggattgggg tcggcgatgt cgtagccgac cagcagcgga
    20821 agcgccacca ggccctgcat cgccgctccg aggttgccgc gcaccatgat cgccagccgg
    20881 ttcaccttgc cggcgaacgt cagcggcacg ccctcgagtt tttcgtagtg ttcgagttcg
    20941 acggcgtaca gccgggcgaa ctcgacggca atcgccgcgg tgccggcgat cccggtcgcg
    21001 gtgtagtcgt cggtgatgta caccttcttg acgtcgcggc tggcaatcat gttgccctgg
    21061 gtggcacgcc ggtcgccggc gatcaccgcg ccgccgggga agttcaacgc gacgatcgtg
    21121 gtgccgtggg ccaactgcac gccagccgct cccgaaacgc cggcaccgct gacaggcagc
    21181 agctgtggcg cctggcggcg caggtattca gaaaaggacg acaagtccag cggtaggggt
    21241 gaagctccgg aattggggtt ggggatggcc agctgttcgc gatacggcca ggtcactgtc
    21301 cgcccttttg gacgtacgcg cgtacgaaat cctctgcgtt ttcctcgagc acgtcgtcga
    21361 tctcgtccag taggtcgtcg gtttcctcgg tcagcttgtc gcgacgctcc tgcccggcgg
    21421 ccgcgttgcc ggtgaggtcg tcgtcatcgc cgccgccacc gccacgcttg gtctgctcct
    21481 gagccatcgc tgcctcctgc atcgttcatc ggcggtctgt aagccgccgg tccttctacc
    21541 ctaccggtca gagcccgaaa tgccggggat gagtcaggtc gtggtgagct gatccacaag
    21601 ctcagcggcc gtctgggcgg actccaacag cgcgccgacg tgggccttgc tgccgcgcag
    21661 cggctctagc gtcgggatgc gcaccagcga gtccccgccg aggtcgaaga tcaccgaatc
    21721 ccagctcgcc gcggcgatgt cggcgccgaa ccgccgcagg cattcgccgc ggaagtacgc
    21781 acgggtgtcg gtcggcgggt tctccaccgc gtcgagcacc tgctgctcgg tgacgagccg
    21841 cttcatcgaa ccccgggcca ccaaccggtt gtagagcccc ttgtccagcc gcacgtccga
    21901 gtactgcagg tcgacgaggt gcagtcgtgg tgccgaccaa ctcaggttct cccggtgccg
    21961 aaatccgtcg agcagccgca gcttggccgg ccagtccagc aggtcggcac actccatcgg
    22021 gtcgcgctcc agcaggtcga gcacattcgc ccaggtctgg acgacgtcgg cggccctcgg
    22081 gtcccggtcg cggctgtcca ccagctttgc cacccgatcc aggtagatgc gttgcagggc
    22141 cagcgcggtc agctcacggc cgtcggccag cgccacggtg gcccgcagcg tcgggtcgcg
    22201 gctgaccacg tgcaccgcgt gcaccggccg cgcaagcgcg aggtcggaca ggtccaaccc
    22261 atgagcgggg ccttcttcga tcagatcgag caccagcgcg gtggcgccga gcttcagata
    22321 cgtcgacgtc tcggccaggt tcgcgtcgcc gatgatgacg tgcagtcgcc ggtacttgtc
    22381 ggcgtcggcg tgcggctcgt cgcgggtgtt gatgattccg cgcttgagcg tggtctccag
    22441 cccgacctcg acttcgatgt agtcggcgcg ctgcgacagc tgaaagccgg gctcgtcgcc
    22501 ggccggtccg atcccgaccc gacccgaccc gacgaccacc tgccgcgaca ccagaaacgg
    22561 tgtcagcccg gcgattaccg cggagaacgg ggtctgccgc gacatcagat agttctcgtg
    22621 cgaaccgtag ctggcgccct tgccgtcgac gttgttcttg tacagctgca gcttggcggc
    22681 acccggcacg ctggcgacat gccgcgccgc ggcctccatc acgcgctcgc ctgccttgtc
    22741 ccagacgacc gcgtccagcg gatcggtgca ctcgggtgcg gagtactcgg ggtgcgcgtg
    22801 gtcgacgtac agccgggcac cgttggtcag gatcatgttc gccgcgccga cctcgtcggc
    22861 gtcgacgatc ggcggcggcc ctgccgaccg ggacaggtcg aatccacgcg cgtcgcgtag
    22921 cggtgactcc acctcgtagt cccatcgggt gcgcttggcg cgctggatgc ccgcggcggc
    22981 ggcgtaggcc agcaccgcct gcgtggacgt caggatcgga ttggcggtcg ggtcggacgg
    23041 cgaggaaatg ccgtactcga cctcggtccc cataatccgt tgcatgtcat cagcctatgc
    23101 gacgtgtatt tacgcttcag cgggcgatga tgtcccaatg ccgacgcccg cctccacccg
    23161 ccgcgccgcc gagcggtggt tcctcgccaa cgggctgccg tccgtgctgc cggggcgggc
    23221 caggtggcgg ggactgtggc cgcgctccgc gccggtgctt gccggatatg cggcattgat
    23281 gatcgtcgga atggtgatct atctgctcgt cggcacgatc aacgtcgaca tcgatcgcga
    23341 accgaccacg gccgagtgga tcgtgctcgg cgtgctcgcc gcgtgcatcc cgttcgtcgt
    23401 cctcaccgga tggctcgtcg cacggctgcg cagctatcgg gcccgggttg ctgcgtcgac
    23461 agtcgctgcc gtggtcgcgg tggtatgtga ctccatcaac gacgggggca cctatgtggc
    23521 caccacggcg ctcggcctgc tgatcgttcc gctgctgact gccacgggca tcgggtcggt
    23581 actgggctgg gcgctccggc tcgcggtttc gcagttcgcg gcggtcggtc agctgatgct
    23641 gcgggcattg ccggtggtgc tgctcaccgt cctggtgttc ttcaactcgc cggtctggct
    23701 gatggcggcg tcggtgaccc gcgcccgcct ttggctagcg ctgttgttcc tgggctcgat
    23761 cgccgcggtg ttccttgtca ccagcaccct cgaacgcatc cggccaatgc tcgtggcgcc
    23821 gaatcctttc gaaacgcaca cgcatgagtt ggcgggcacg ccgttcgagt cgatgaacga
    23881 cccggccacc gccgaaccac tgacgcttcg cgagcggttc aacgtggtgt tcgtcgtgat
    23941 cgtgtcccag ttggcgcagg tgctcaccgt ggccgtcgtg accgcgctga tcttctttgt
    24001 gttcggcctg atcctgctca accctgaact gctcgcggcg tggacgcgca accccatgag
    24061 tgacgggcgt ctgctgtgga tgacggtgcc ggtgccgaac gcactgattc aggtcacgct
    24121 gtttctcggc gcgctgacgt tcatgtacct cagcgcgaga gcggtcagcg acggcgagta
    24181 ccggtcccgc ttcgtggatc cgctgatcga cgatctgaag ctgacactgg tggcgcgcag
    24241 ccgctaccgc gctgcgacgg cgggcgaatg acggacctca aggcggtgca cgagtggttc
    24301 ctgcagcgcg ggttgccgct ggtgttgacc cgtcgggtcc gcgcccgggc gctgatcgag
    24361 cgctcggcgc cgatggtcgg tggcgtcggc gcgctggtgg cggtgctgtt gctgctcgcc
    24421 gacgtcacgc acgatcgcac gaccccggta cacgttgtcg tcgtcgccgc ttacgcggct
    24481 gtgctcgcgg ccacgccagc gctgctgtac ctgttgcacc gcatcggcac accggtcagc
    24541 gaggccaggc ggcgctcggt ggcatggctg gtgcttcctc tgttcgtgct gggccttccg
    24601 gtaatcgacg cgggctggtc gtggacggcg ttagccgaag ccccggtctt tgtggtcgcg
    24661 tcgctgctgg cgatctggct gacttacctt gggatcggct caattgtgtt gtgggcgttg
    24721 cggtttgcgt ctgtacagtt cggcgcattg gggacgctga tgagcggtgc gctgcccctg
    24781 ctgatgatca ccgtgctggt gttcttcacc gctgacctgt ggcagctcac cgcgcggctg
    24841 acccgagacc ggctgtggca gacagtgggt ttcctggcgc tggtcgccgt cgcattcatg
    24901 gtcgtgacca tccaggacga ggtccgctcg ctgcgcaaag gccgggccgc aacgaagcca
    24961 gcacctgagc tgttgaaggg cactccgctg gccatgtact cagacggtga tctggctacc
    25021 gccccccccg gtgtcacgac cagagaaggt gaatgtgctt gcggtcatgg tggtttcaca
    25081 ggcgattcag gtcgtgctgt tcaccgcggt gatcttcgcg ttctttctga cgctcgggat
    25141 catcgccgtg cccgacgatg tggcggtggt gtggtcgcag gagacgacgt gcccagatgg
    25201 tgcccaacca ccttgcgccg gaacgtggtt cggtatcaag acgccgctga cgcagacgct
    25261 ggtgcagacc tcgttgttcg tcgcggtgct gtcggggctg tacttcaccg tcaacagcag
    25321 cgtcgacccg cagtaccgca agcggttctt cgaaccgctg atcgcggacc tggccgtcag
    25381 cctggccggc cgtgacgtgt accggtcact cgacaaggcc cactgaagcc tccacaaggc
    25441 tgaacggcga cgctgtcggc accacgcgcg tcatcttgaa tccggagcgc tcaagcagtg
    25501 ttcggtactg tgccgcggtg cgctcgcggc cattcacacc ctgcagcatt tcgagatcgg
    25561 cccatttgcc gatgaaatcg cggtcgtgct cgggaattac gagttcggcc agcaacacgg
    25621 tggcggccgc attgcaggcg gcgcgaagtt tgcgcaagat caccacagct tcgtcatccg
    25681 gccagtcgtg aatgatgtgc ttgaggacgt aagtgtctcc gcccgcgggg atatcttcga
    25741 aaaatgatcc cgcctctatg cgaacctgtc ctgccacacc gagtttgtgc agctgcgccg
    25801 gcgctgcgga gacgacgtct ggcaagtcgt agagcacacc gcgagcggtc ggcgccgccg
    25861 acaggatggc cgccagcagg cggccgtgac cgccaccgac atccacgatc atccggcatg
    25921 gcgtgaagtc gtaggcggcg acaatggtct gctcggccat ctccgaaatg ctggtcatag
    25981 cggcgttgaa aagcgctgcc agttgggcgt cgtcagtcag gtaatcgaaa aatcccttgc
    26041 cgcgcagcgc cgggatggtc gccttgcctg tcctgacgga ctcggtgagt gtgctccagt
    26101 gctcgcgatg ctgcggcgac ccgtagaaca tcgccgctcc cgccatcgat accggcgcgt
    26161 ctgcccgcag ggtgtccgcg agcgcattga gggcataggt gccgtcgcgg cgttggcgaa
    26221 atacgccggc tgatcaaagc ccgcatgagt cggttcatcg cgtcagggtc cgcgccgacg
    26281 cggcgcgcca aatcatcacc tgtgagcggg cctgcggcga gcgcgtcggc gattccgagc
    26341 tccgcggcag cctcgatcgc ctgtgacatc cagcccgcca gaatcagaaa catcacgacc
    26401 ggcgccgggg ccagcctctg atggatgcga tagaggcgat gccgcaggcg ctcagtcagt
    26461 ctcaccaacg gaaccggtgg aaccgtacgt cgtgccatca catgggttgc catggcaacc
    26521 tccgtagtat ggttagatca atgaaactct attcaataga gttaactata tggaagggcg
    26581 catgctcgtg tccaccactc gtcgggcacc atcccgccgc acgtacgaca acagtcgccg
    26641 ccgagccgac gcagaagtcc gt
//
LOCUS       DASWBF010000553         5891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1174173,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000553 DASWBF010000000
VERSION     DASWBF010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174173"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VF874_20720"
     CDS             <1..491
                     /locus_tag="VF874_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9178391.1"
                     /translation="ALRALGNDIPICVLSARDTVNDRIAGLEAGADDYLVKPFDLGEL
                     VARLHALLRRSTHSDEHSDAITIGPLTIDTARRLVFVAGERAELTKREFDLLAVLAEN
                     AGVVLTRQRLLELVWGYDFDVDTNVADVFISYLRRKLEREGVPRVIHTVRGIGYVLRA
                     EP"
     gene            585..1964
                     /locus_tag="VF874_20725"
     CDS             585..1964
                     /locus_tag="VF874_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9178392.1"
                     /translation="MSLRTRVAIAAAAAAAAVVALFAVVTSVLLVNNDAAQLDRRLDA
                     IVDTSIDPDRNAAEPRAILQTVRNPTTEQVMYQRGFQLPPLPVGTSTVSVNGVDYRVR
                     TVEREQPDGDVLVSIGIRVDSILVDRARIPLYLLIGGVAVLVAAVLGWLLAGPAVRPL
                     RRLTQHTRRLGKGGERLPAVHGIREAEDLSEAMSGMLERLAAAQQSTTNSLQTAQDFA
                     ANAAHELRTPLTAMRADLDTLRIHDLPADERQEVVADLSRAQRRVEAIITALGQLASG
                     ELAQVEDREPIDVADLLERVAQDHMRPTMPVEIEVRADEAGTIFGWPSGLRLAVDNMV
                     RNATTHGGATRVVLTATRRGDLLDIVVDDNGRGLPAEEHVTVLGRFARGSTASPGGSG
                     LGLALVAQQAVLHDGDIALSDGPLGGLRATLTVSTRGGPKSDPALPRRRPASRAGKSP
                     SPPKRRSRK"
     gene            complement(1861..2238)
                     /locus_tag="VF874_20730"
     CDS             complement(1861..2238)
                     /locus_tag="VF874_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HEX9178393.1"
                     /translation="MNAKQVWSAVLADALCVLLFCAIGRRSHAEGVTVSGVAETAWPF
                     LVGAAVGWLVSRGWRRPLAITPTGVAVWACTIAVGMLLRKATSGGVAVTFVIVASVVT
                     AIFLLGWRGVAAVVPDRISGRRG"
     gene            complement(2235..3383)
                     /locus_tag="VF874_20735"
     CDS             complement(2235..3383)
                     /locus_tag="VF874_20735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9178394.1"
                     /translation="MTVTETAGPTGAAPAGPTVFTPVVGSVLAPPMPVATTDAKTHLA
                     YELVLTNAGPQEVTLTSVAAVAGDKTLLTLSGDELANWTRRLGNPAPTTTIRPSETAL
                     VWLDVAIDEAAGSPSDISHTIAMSVANPQPPLTEATMTETIAATTVDNRKPVTISPPL
                     RGENWLDGDSCCGMTAHRMAVSPLNGALWAPERYAIDYVQLSSDGRMFNGDKGNLASY
                     PYFGADVLAVADGPVVAVVDGLAEQVPGANPRGLPLDQYAGNRVVQDIGGGNYVLYAH
                     LKTGSVKVKVGDRLTTGQTLGSLGNTGNSDAPHLHFELMSTPDPLRSNGLPFVFDKFR
                     LDSRIASTDALNNLTDTGGPAERQHGVAARDQTLVMPLVLDVMTYPAR"
     gene            complement(3519..4643)
                     /locus_tag="VF874_20740"
     CDS             complement(3519..4643)
                     /locus_tag="VF874_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEX9178395.1"
                     /translation="MSQDAPASDARSGEGQSRGKYWWLRWAVLGVAAVVLVVELALVH
                     DQLAQAWRSLVTAKWYWVLAAIAAALASMHSFAQIQRTLLQSAGVTVKQWRSETAFYA
                     GNALSTTLPGGPVLSATFIYRQQRLWGASPVVASWQLVMSGVLQVVGLALLGLAGAFL
                     LGAKNNPFSLIFTLGGFIALILLAQAVASRPELIDGIGVRVLSFVNSVRGRPRDAGLD
                     KWRETLTQLESVSLSRRDLSVAFGWSLFNWIADVACLLFATFAAGGRPSLAGLTVAYA
                     AARAVGSIPLMPGGLLVVEAVLVPGLVTSGVSLPDAISAMLIYRLISWIFIAAIGWVL
                     FLFLFRTESQIDPDAAERGGIDAEPKPSANGDAPDDDGDP"
     gene            4738..>5891
                     /locus_tag="VF874_20745"
     CDS             4738..>5891
                     /locus_tag="VF874_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9178396.1"
                     /translation="MPAPEDTAVSPLVRKGAAWSWRLLAIFGLIFAMLWVFKHLELIF
                     VPVAIATMLAALFLPGVDWLDRHGVPRGLSVFLLLLFGMALVGGVLAFVVSQFIEGLP
                     GLVDQVTHSIETAKNWLIQGPAHLNEQQIDNAGKSAVEAIRNNQEKLTSGALSTAGTI
                     TEIVTGAFVVLFTLIFLLYGGRNIYEFVTRIFPPTVRQKVREAGKAGFGSLIGYVRAT
                     FLVALVDATGIGTGLAIMGVPLALPLASLVFLGAFIPLVGAVIAGFLAVIVALLSKGF
                     IYALITLGLIIAIQQLEGHVLQPLVMGRAVSIHPLAVVLGISTGGVLAGIVGALLAVP
                     AIAFANSAIRVLMERDEDVGGPGEKPDYDEPVGISAEPDDVGAGAEKPSGT"
BASE COUNT          967 a         1988 c         2008 g          928 t
ORIGIN      
        1 cggcgctgcg cgcgctgggc aacgacatcc cgatctgcgt gctgtcggcc cgcgacaccg
       61 tcaacgaccg catcgcgggg ctggaggccg gcgccgacga ctacctggtc aagccgttcg
      121 acctcggcga gctcgtcgcc cggctgcatg cgctgctgcg ccggtccacg cattccgacg
      181 agcactcgga cgcgatcacc atcggcccgc tgaccatcga taccgcgcgc cgcctggtgt
      241 tcgtcgcggg ggagcgagca gagctgacga agcgcgagtt cgacctgctg gccgtgctcg
      301 cggagaatgc cggtgtggtg ctcacccggc agcggctgct ggagctcgtc tggggctacg
      361 acttcgacgt cgacaccaac gtcgctgatg tgttcatcag ctacctgcgg cgcaagcttg
      421 aacgcgaggg cgtaccgcgg gtgatccaca cggttcgcgg gatcggatat gtgctccggg
      481 cggagccgtg aggcgactgg acggcacgga gccgtgaggc gactggacgg cacagcgccg
      541 tgaaaggcag cgtcgaacgg cttcgaatac ggctgcaccg gtcgatgtcg ctgcgcacgc
      601 gggtggccat cgctgcggcg gcagcagcag cggccgtcgt cgcgctgttc gcggtggtga
      661 cgtcggttct gctggttaac aacgacgcgg cgcagctgga ccggcggctc gacgcgatcg
      721 tcgacaccag catcgaccca gaccgcaatg ccgccgagcc gcgcgcgatc ctgcagaccg
      781 ttcgcaaccc caccaccgag caggtgatgt accagcgcgg cttccagctg ccgccattgc
      841 cggtgggcac ctcgacggta tctgtgaacg gggtcgacta ccgtgtccgt accgtcgagc
      901 gggaacagcc agacggcgac gtgctggtgt cgatcggcat cagagtcgac agcatcctgg
      961 tggaccgcgc gcggattccg ttgtacctgt tgatcggcgg cgtcgcggtc ctggtcgccg
     1021 cggtgttggg ctggctcttg gccggcccgg ccgtgcggcc gttgcgccgg ctcacgcaac
     1081 acacccgccg cctcggcaag ggcggcgaga ggctgcccgc cgtgcacggg atccgcgagg
     1141 ccgaggacct ctcggaggcg atgtccggga tgctcgaacg gctggcggcg gcgcagcaat
     1201 cgaccactaa ctcattgcaa accgcgcagg acttcgccgc aaacgcggct cacgaactgc
     1261 gcacgccgct gaccgcgatg cgcgccgatc tggacactct gcgtattcac gacctccccg
     1321 ccgacgaacg acaggaggtg gtagccgatc tgtcccgcgc gcagcgccgg gtggaagcca
     1381 tcatcaccgc cctgggccaa ctggcgtccg gagagctggc ccaggtcgag gatcgcgaac
     1441 cgatcgacgt tgccgacctg ttggagcggg tggcccagga ccacatgcgc ccgacgatgc
     1501 cggtcgagat cgaggtgcgg gccgacgagg ccggcacgat cttcgggtgg ccgagcgggc
     1561 ttcggcttgc ggtggacaac atggtccgca atgcaaccac ccacggcggc gcgacacggg
     1621 tcgtgctgac cgcaacccgg cgtggcgacc tgctggacat cgtcgtcgac gacaacgggc
     1681 gcggccttcc cgctgaggag cacgtcacgg tgctgggccg gttcgcccga ggcagcaccg
     1741 cgtcgccggg aggctcggga ctcgggctgg cgctcgtcgc gcagcaggcg gttcttcacg
     1801 acggcgacat cgcgctctcc gacggaccgc tgggtggttt gcgtgcgacg ttgacggtgt
     1861 ctacccgcgg cggcccgaaa tccgatccgg cactaccgcg gcgacgcccc gccagccgag
     1921 caggaaaatc gccgtcacca ccgaagcgac gatcacgaaa gtaaccgcta cacctccaga
     1981 agtggccttg cgcaacaaca ttccaaccgc gatcgtgcac gcccacaccg cgacgccggt
     2041 cggggtgatc gccagcggac ggcgccagcc gcggctgacg agccaaccca ccgcagctcc
     2101 gaccagaaac ggccaggccg tctctgcgac ccccgagacc gtcacgccct cggcgtggct
     2161 tcgccggccg atcgcgcaga acaacagcac gcacaacgcg tcggccagca ccgccgacca
     2221 cacctgcttc gcgttcatcg ggccgggtag gtcatcacgt ccaagaccaa tggcatgacg
     2281 agggtttggt cacgtgcagc aaccccgtgt tgtcgttcgg ctggtccgcc ggtgtcggtg
     2341 aggttattga gcgcatcggt ggacgcgata cgcgaatcga gccggaactt gtcgaacacg
     2401 aagggtaaac cattggaccg caacgggtct ggtgtgctca tcagctcgaa gtgcaggtgc
     2461 ggcgcgtcgg agttgccggt attgcccaag gagccaagcg tctggccagt ggtgagccgg
     2521 tcacccacct tgaccttgac gctgccggtt ttcaggtgcg cgtagaggac gtagttgccg
     2581 ccgccgatgt cctgcaccac acggttaccg gcgtactgat caagcggcaa acctcgagga
     2641 ttggcgcctg gcacttgctc ggccaagccg tcgaccaccg cgaccacggg tccgtcggcg
     2701 accgcgagca cgtcggcgcc gaagtacgga tagctcgcga gatttccctt gtcgccgttg
     2761 aacattcgcc cgtcagagga tagctggacg tagtcgatcg cataacgttc gggtgcccag
     2821 agtgctccgt tgagcgggct gactgccatc cggtgtgccg tcatcccgca gcagctgtcg
     2881 ccgtccaacc agttttctcc gcgcagcggc ggtgagatcg tcaccggctt gcggttgtca
     2941 accgttgtgg cagcgatggt ttcggtcatc gtcgcctccg tcagcggagg ctgcgggttt
     3001 gccacggaca tggcgatggt gtggctgata tcggaggggc tgcccgcagc ctcgtcgatc
     3061 gcgacgtcga gccacaccag cgctgtctct gacgggcgga tcgtcgtcgt cggcgccggg
     3121 ttgcccagcc gccgcgtcca gttcgccagc tcgtcaccgg acagcgtcag cagggtcttg
     3181 tccccggcga ccgccgccac agacgtcagc gtgacctcct gaggtccggc gttcgtcagc
     3241 accagttcgt aggccaggtg cgtcttggcg tcggtggtgg ccaccggcat tggcggggcg
     3301 agcaccgagc cgacgaccgg ggtgaaaaca gtcgggcccg ccggtgcggc acccgtcgga
     3361 cccgcagtct cggtgacggt cactgtggac ggagacggag actggcccga ttgcgtctgg
     3421 gtggcgcacg acgtcagaac gagtaccgcg cttatcgcgc atgctgaaaa gcgcaggtgc
     3481 ataccagtat ccaagcacgt gcaggtaccc gacgagggtc aagggtcgcc gtcgtcgtcg
     3541 ggggcgtccc cgttggcaga tggcttcggc tcggcatcga tgccgccgcg ctcggcggca
     3601 tccgggtcga tctggctctc ggtgcggaac aggaacaaga acagcaccca gccgatggcc
     3661 gcgatgaaga tccagctgat cagccgatag atcagcatcg ccgagatcgc gtccggcagt
     3721 gacaccccgc tggtcaccag gcccggcacc agcacggctt cgaccaccag cagcccgccg
     3781 ggcatcagcg gaatcgaccc gacggcgcgg gcggcggcgt aggcaacggt caagccggcc
     3841 agcgacggcc ggccaccggc ggcgaacgtc gcgaacagca gacacgccac gtcggcgatc
     3901 cagttgaaca gcgaccatcc gaacgcgacc gaaaggtcgc ggcggctcag gctgaccgac
     3961 tccaattggg tgagcgtctc gcgccacttg tccaggccgg cgtcgcgggg ccggccacgg
     4021 accgagttca caaacgacag gactctcacc ccgatgccgt cgatcaactc cgggcggctg
     4081 gcaacggcct gggcgagcag gatcaacgcg atgaacccgc cgagagtgaa gatcagcgag
     4141 aacggattgt tcttggcgcc caacaggaat gccccggcca ggcccagcag cgcgagacca
     4201 acgacctgca gcacccccga catcaccagc tgccacgacg caacgaccgg cgaagcgccc
     4261 cacaggcgtt gctggcggta gatgaaggtc gcggacagca ccgggccgcc cggcagtgtg
     4321 gtgctcaacg cgttgcccgc gtagaacgcg gtctccgacc gccactgctt gacggtgacg
     4381 ccggcggact gcaacagcgt gcgctggatc tgcgcgaagc tgtgcatcga ggccagcgcg
     4441 gccgcgatcg cggcgagcac ccagtaccac ttcgcggtaa ccaggctgcg ccaggcttgg
     4501 gcgagttggt cgtggaccag cgcgagttcg acgaccagca ccaccgccgc gacaccgagc
     4561 accgcccagc gcagccacca gtacttgccg cgtgactggc cttccccgct gcgggcgtcg
     4621 cttgccggtg cgtcctgcga cacgtgctac aggctaaccg cgaaggcgca gccgccatcg
     4681 cgggctcgct ggcggcgaac cggcttttcg ccgcagcgct gtggttacgc taccggcatg
     4741 ccagcgcccg aagacaccgc cgtttcgccg ctagtgcgca agggcgccgc ctggtcgtgg
     4801 cggctgctgg cgatcttcgg gttgatcttc gcgatgctgt gggtgttcaa gcacctcgag
     4861 ctcatcttcg tgccggtggc gatcgcgacg atgctcgccg cgctcttcct gcccggcgtc
     4921 gactggctgg accggcacgg cgttccccgc ggcttgtctg ttttcctgct gctgctgttc
     4981 gggatggcgc tggtcggcgg tgtcctcgcg ttcgtcgtca gccagttcat cgagggcctg
     5041 ccggggctgg tcgaccaggt tacgcacagc atcgagaccg cgaagaactg gctcatccag
     5101 gggccggctc acctcaacga gcagcagatc gacaacgcgg gcaagtccgc ggtcgaggcg
     5161 atccgcaaca atcaggagaa gctgaccagc ggcgcgctgt ccaccgcggg cacgatcacc
     5221 gagatcgtca cgggcgcgtt cgtcgtgttg ttcacgctga tcttcctcct gtacggcggc
     5281 cgcaacatct acgaattcgt cacccggatc ttcccgccca ccgtccggca gaaggttcgc
     5341 gaagcgggca aggccgggtt cggctcgctg atcggctacg tccgcgcgac gttcctcgtc
     5401 gcgctggtcg acgcgaccgg catcggcacc ggcctggcga tcatgggcgt gccgctggcg
     5461 ctgccgctgg cctcgctggt gttcctgggc gcgttcatcc cgctggtcgg cgcggtgatc
     5521 gccgggtttc tggctgtcat cgtggcgctg ctgtccaaag gcttcatcta cgcgctgatc
     5581 accctcggcc tgatcatcgc catccagcaa ttggaggggc acgtgctgca gccgctcgtg
     5641 atgggacggg ccgtgtcgat tcacccgctc gccgttgtcc tcggcatttc gacgggtggc
     5701 gtgctggcgg gcatcgtagg ggcgctgctg gcggtgcccg cgatcgcgtt cgccaacagt
     5761 gcaattcgcg tactgatgga acgcgacgag gacgtcggcg gacccgggga gaaaccggac
     5821 tacgacgagc cggtaggcat cagcgccgag cccgacgacg tcggcgcagg ggctgaaaag
     5881 ccgtcgggga c
//
LOCUS       DASWBF010000554         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1246398,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000554 DASWBF010000000
VERSION     DASWBF010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246398"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1171
                     /locus_tag="VF874_20750"
     CDS             41..1171
                     /locus_tag="VF874_20750"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX9178397.1"
                     /translation="MSCSLVAAAALGCAAATSAAAAPTTKVSVVVNGLNNPRGLTWAH
                     GRLYLAEAGKGGTDCPTGAKGPTGGQLCYGLTSSLDVISRGSVRAIRRGLISFSDSPG
                     GAAAEGVEAVTGVARGVQVVYGESVLAHLFGLPSGTHLTPADSAALRHEAGMLHSVIG
                     TSERNLADVGDSDLAWSGVHQNLVPDQFPDANPNALLHLGRLTYVIDAGTNVLSSVDP
                     RGVVRQLAFFPNPAQSDAVPTCVAKGPDNALYVGQLAPGAGPNQGKIYRYSLSTGRLT
                     VWKTGFNVVDGCGFDHAGNFYAVEFQAHGFNPSPTGNPAGDIIKISRNGKRSVIGAGS
                     LFFPQGFAVDPKGNIYVSNWSIMTDTPSRPGGPTGEVVKISP"
     gene            complement(1621..>2863)
                     /locus_tag="VF874_20755"
     CDS             complement(1621..>2863)
                     /locus_tag="VF874_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005563045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9178398.1"
                     /translation="ADDVARSIAFARAHEARIAVRGGGHNGGGLGIVDDGVVIDLAGM
                     AEVVVDPDANTVRVGGGCTWGDVDRVTAEYGRATPSGIISTTGVGGLTLGGGLGHLTR
                     PFGLTIDSLLGAEVVLADGSRVYASANEHPELFWALRGGGGNFGVVTSFEFRTHPVGA
                     AVMAGPTFWPIEQTPEVMRFYREFIPAAPRNVGGFFAEMTVPPVELFPEELHLRKVCA
                     VMWCVVGSEEDATRLLAPVHDVGTPLLHGVGPVPYPALQSLFDGLYTKGLQCYWRADF
                     VNELSDELIEQHLEWAQKLPSMHSTMHLYPIDGAAHDVGAADTAFSYRDAQWAEVIFG
                     VDPDPANAGAIRDWCVGYWDATHPYSAGGAYVNFMMDEGQERVRATYRDNYDRLARVK
                     AQYDPDNVFQVNQNIHPAASA"
BASE COUNT          497 a          958 c          947 g          461 t
ORIGIN      
        1 caggactgaa aggagtggta tgaagcgtaa gagaaggatc atgagttgca gccttgtggc
       61 ggcagcagct ctcggatgcg cggcggcaac gagtgccgct gccgcaccca cgacgaaggt
      121 ctcggtcgtg gtcaacggtc ttaacaatcc gcgaggactg acctgggcgc acgggcgcct
      181 atacctcgct gaggccggga aagggggaac ggactgcccc acgggcgcta agggtcctac
      241 gggtggccag ctgtgctatg gcctgaccag cagcctcgat gtgatctcac gcgggtcggt
      301 gcgcgcgatc cgccgcggcc tgatctcgtt ctccgactcg cctggcggag cggcggcgga
      361 aggcgtcgag gcagtgaccg gggtggcccg cggcgtgcag gtcgtctacg gcgaatctgt
      421 gctggcccac ctattcgggt tgccctcggg cacccatctg acacccgcag acagtgccgc
      481 actcaggcat gaggcgggaa tgctccactc cgtgatcggg accagcgagc ggaacctcgc
      541 ggatgtcggt gactcggatc tggcctggag cggggtccat cagaacctcg tgcccgacca
      601 gttcccggac gcgaacccga acgcgctcct gcatctcgga cgcctgacgt acgtgatcga
      661 tgccggcacc aatgtgctca gctcggtcga cccgcgcgga gttgttcgac agctggcgtt
      721 cttcccgaac ccggcgcaaa gcgacgcggt gccgacctgt gtagcgaagg gtcccgacaa
      781 tgcgctctac gtcgggcaac ttgcccccgg cgccgggccc aaccagggaa agatctaccg
      841 ctacagcctg tccaccgggc gcttgaccgt gtggaagacc ggtttcaacg tcgtcgatgg
      901 ttgcggcttt gaccacgctg ggaacttcta cgccgtcgag ttccaggccc acggctttaa
      961 ccccagcccc accgggaatc ctgccggaga catcatcaag atcagccgca acggaaagcg
     1021 cagcgtgatc ggcgccggca gcttgttctt cccgcaggga ttcgccgtcg acccgaaggg
     1081 caacatctac gtgtccaact ggtcaatcat gacggacacc ccgagccggc ctgggggccc
     1141 gaccggcgaa gtcgtcaaga tcagcccttg atattcggcc gtgcgcgcgg tctggcggtt
     1201 gccgcggcca accgccagcc gcgcggcggt ccgccgtacc gttgcccggc ggctgacggt
     1261 accggaacga gctctcaacc ggtgatgcgg ccgaacgcgc caaacgccag ctaggagcta
     1321 cccagtcgcc gagagtgttg gcttgggcta agagcagcat cgggtcagca tcgggtcgcg
     1381 cttgcgcagg aagtgctggg acacccaagc acgagactgc ccgtcctcga tgcgaagcag
     1441 cactctcccg gtacacatcg aggctggcgg tggcgttccg aggcgagaga tcgctgaagg
     1501 gcgaccgaca ctgctgcatt gatcccggtc tgctggctga ccgcacgacg ctgcaagcaa
     1561 ccggggcgct tgcagcgtcc tgcccgcgca gcttatcgcc gggggccgct cgcgatcttc
     1621 ctatgcgctg gccgcggggt ggatgttctg gttgacctgg aagacgttgt cggggtcgta
     1681 ttgcgccttg acgcgcgcga gtcggtcgta gttgtcgcgg taggttgcgc ggacgcgctc
     1741 ctggccctcg tccatcatga agttgacgta cgcgcctccg gctgagtagg ggtgggtcgc
     1801 gtcccagtag ccgacgcacc agtcacggat cgcgccggcg ttggccgggt ccgggtcgac
     1861 gccgaagatg acctcggccc attgcgcatc gcggtagctg aaagcggtat ccgcggcccc
     1921 gacgtcgtga gcggcgccgt cgatcggata gaggtgcatc gtcgaatgca tcgacggcag
     1981 cttctgcgcc cactcgaggt gctgctcgat cagctcgtcg gacagttcgt tgacgaagtc
     2041 ggcgcgccag tagcactgca agcccttcgt gtagagcccg tcgaacaggc tctgaagcgc
     2101 tgggtacggc accggtccga cgccgtgcag gagtggcgta ccgacgtcgt gcacgggtgc
     2161 cagcagccgt gtggcgtcct cctcggagcc gacgacgcac cacatcaccg cacagacctt
     2221 gcgcagatgg agctcctctg ggaagagttc caccggaggt accgtcatct ctgcgaagaa
     2281 gccgcctacg ttgcgcggag ccgcgggaat gaactcgcga taaaagcgca tcacctccgg
     2341 cgtctgctcg atcggccaga acgtcggccc ggccattacc gcagcgccga ccgggtgcgt
     2401 gcggaattca aacgacgtaa cgacaccgaa gttgccgccg ccaccgcgca acgcccagaa
     2461 cagctcggga tgctcgttcg cactcgcata tacccgtgac ccatcggcga ggaccacctc
     2521 agcaccgagc aggctgtcga tcgtcaggcc gaacggccgc gtcaggtgcc cgagcccgcc
     2581 gcccagcgtg agcccgccaa cgccagtcgt cgagatgatc cccgacggcg tcgcgcggcc
     2641 gtactcggcg gtcacccgat cgacgtcgcc ccacgtgcag ccgccgccga cccggacggt
     2701 gttggcgtcc ggatcgacca caacctcagc catgcccgcc aagtcgatga caacgccgtc
     2761 atcgacgatg ccgaggccgc cgccgttatg cccaccgccg cgcactgcga tccgcgcttc
     2821 atgggcacgc gcgaacgcga tgctgcgtgc gacgtcgtcg gcg
//
LOCUS       DASWBF010000555         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_389368,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000555 DASWBF010000000
VERSION     DASWBF010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389368"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VF874_20760"
     CDS             <1..718
                     /locus_tag="VF874_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX9178399.1"
                     /translation="SLGVETAGGVMTKIIERNTTIPARRSEVFTTAADNQPAVDIVVL
                     QGEREMAKDNRVLGRFKLENIRPAPRGEPQIEVTFDIDANGILHVTARDKDTGAEQSI
                     TISEQSNLDQSEVERMLAEAEAHRREDEQLRKQVDARNELDSVAYQVERRLAELGDSA
                     APHDRARAEMLVADARQAVKDEAPLERVQSLTSELQQVLYGLQPAPAGGGNGQQQPSG
                     NDQGSRVGDDDVVDAEFDRG"
     gene            725..1249
                     /locus_tag="VF874_20765"
     CDS             725..1249
                     /locus_tag="VF874_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005180075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9178400.1"
                     /translation="MVSSTEHSKTDEDRDAVGESPPEAVASQTDSDAELAKLEDRWRR
                     AVADLDNLRKRYARELDRERTTERSRVAGAWLPVVDNLERAIAHSGDQSDAVVEGVRS
                     ILEQALQVLEQLGYPRDAEAGVPFDPERHEVVGVVDHPDSAPGTVVEVLRPGYGEGSR
                     QLRPAAVVVSRREE"
     gene            1256..2170
                     /locus_tag="VF874_20770"
     CDS             1256..2170
                     /locus_tag="VF874_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX9178401.1"
                     /translation="MARDYYEVLGVSRDATADQIQQAFRTLARKHHPDVNKDPAAEER
                     FKEVNEAYHVLSDPETRKRYDRFGEDFRKVPEDWEERVGAGAGGFGGAGPSGARVRYG
                     QGFGGAGGINIEDLFGDMFGGSGGFGPIPGADQEAVLELTVEEAYRGGKRQLSLDGRN
                     YGVNIPAGVIDGQRIRLAGEGGRGSGDGPAGDLYLRVRIKSHPRFRLDGRDVTVDLPV
                     SPWEAVLGTTVAIRTPGGEAKVKVPQGSSTGRRLRLRGEGMPNPRGAAGDLYAEIKVM
                     VPPKPTARERELFEQLAAESTFDPRRGR"
     gene            2167..2484
                     /locus_tag="VF874_20775"
     CDS             2167..2484
                     /locus_tag="VF874_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005259200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEX9178402.1"
                     /translation="MTAPRYVLARRPGIQLDVFATRCGLHPDMVRRLVALGLVACQQD
                     ARGDLWFEPSALVTVARIQRLRTGLGLNYAAIGLVLDLLDRIEELESASRRRRTSPWT
                     STS"
     gene            2466..>3347
                     /locus_tag="VF874_20780"
     CDS             2466..>3347
                     /locus_tag="VF874_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX9178403.1"
                     /translation="MDINKLTQKSQEALAEAQSIATRMGHIEVDGEHLLMALIDQPEG
                     LVPRLLDQTGADTAALRADLERELNRRPKVSGPGAAPGQVSVTRRLANVLEAAEREAK
                     RLKDEYVSVEHLVIALAEEGTSTAAGRILANHGVTRDSFLSALTKVRGNQRVTSATPE
                     GSYEALEKYGRDLVAEAQSGKLDPVIGRDAEIRRVVQILSRKTKNNPVLIGDPGVGKT
                     AIVEGLAQRIVRGDVPEGLRDKTIFSLDMGSLVAGAKYRGEFEERLQAVLSEVKAGEG
                     RILLFVDELHTVVGAGAT"
BASE COUNT          621 a         1037 c         1208 g          481 t
ORIGIN      
        1 ctcgctgggt gtggagaccg ccggcggcgt gatgaccaag atcatcgagc gcaacaccac
       61 catcccggcc cggcgcagcg aggtgttcac caccgcggcg gacaaccagc cggccgtaga
      121 tatcgttgtg ctgcaaggcg aacgcgagat ggccaaggat aatcgcgtac tcggcaggtt
      181 caagctggag aacatccgac cggccccgcg cggtgagcca cagatcgagg tcactttcga
      241 catcgatgcc aacgggatcc tgcatgtcac cgcgcgcgac aaggacaccg gcgccgagca
      301 gagcatcacc atcagcgaac agtcgaacct cgatcagagc gaggtcgagc ggatgctcgc
      361 cgaggccgag gcgcaccgcc gcgaggacga acagctgcgc aagcaggtcg atgcacgcaa
      421 tgaactcgat tcggtggctt accaagtcga gcgccggctc gccgagctag gcgactccgc
      481 ggcgccacat gaccgggccc gcgccgagat gctggtcgcc gacgcgcggc aagccgtcaa
      541 ggacgaggcc ccgctggagc gggtgcaatc gctgacatcc gaactgcagc aggtgctcta
      601 cgggctgcaa ccggccccag ccggcggcgg caacgggcag cagcagccgt ccggtaacga
      661 ccagggctcg cgagttggcg acgacgacgt ggtcgacgcc gagttcgacc ggggctgagc
      721 cgccatggtc agttcgacag aacattcgaa gaccgacgag gaccgcgacg cggttggcga
      781 aagcccgcct gaagcggttg cctcccaaac cgattcggac gccgagctgg ccaagctcga
      841 ggatcgttgg cgacgcgcgg tcgcggatct ggacaacctg cgaaagagat atgcccgcga
      901 actggaccgc gaacgaacaa cggaacggtc cagggtggcc ggcgcctggc tgcccgtcgt
      961 ggacaacttg gagcgggcga tcgcccactc gggtgaccag tccgacgcgg tcgtcgaggg
     1021 cgtgcggagc attctcgaac aagcgctaca ggttctcgag cagctcggct atccgcgcga
     1081 tgcggaggca ggggtgccct ttgacccgga gcgtcacgag gtggtcggtg tggtggacca
     1141 cccggacagc gcgcccggga cggttgtcga agtacttcga ccgggctacg gcgaaggatc
     1201 gaggcaactg cggccggcgg ccgtggtggt cagccgacgc gaggagtgag atcgggtggc
     1261 ccgcgactac tacgaagtcc tcggggtgtc gcgggacgcg accgccgacc agatccagca
     1321 ggcgttccgc acactggccc gcaagcatca cccggacgtc aacaaggatc cagcggccga
     1381 agaacggttc aaagaggtca acgaggccta tcacgtgctg tcggaccccg aaacccgcaa
     1441 gcgctacgac cgattcggcg aagacttccg gaaggtgccc gaggactggg aagagcgggt
     1501 gggcgccggt gccggtggct tcggcggcgc agggccctcc ggcgcgcggg tgcgctacgg
     1561 ccagggcttc ggcggcgccg gcggcatcaa catcgaagac cttttcggcg acatgttcgg
     1621 cggcagtggc ggattcgggc cgattccggg tgctgaccag gaagcggtgc tggagctgac
     1681 tgttgaggag gcttaccggg gcggcaaacg gcagctctca ctggatggcc gcaactacgg
     1741 ggtcaacatt cccgccggtg tcatcgacgg tcagcggata cggctggcgg gagagggcgg
     1801 ccgaggtagc ggcgatggac cggccggaga tctgtacctg cgggtgcgca tcaagtcgca
     1861 tccccgattt cggctcgacg gacgcgacgt caccgtcgat ctgccggtgt cgccgtggga
     1921 ggcggtgctg gggaccactg tcgctattcg aactcccggt ggcgaagcga aagttaaggt
     1981 gccgcaaggg tcgtcgaccg gtcggcggtt gcggctgcgc ggcgaaggca tgcccaaccc
     2041 gcgcggtgcc gccggcgacc tctacgcaga aatcaaggtg atggtgccac cgaaacccac
     2101 tgcgcgggaa cgtgagctgt tcgagcaact ggcggcggag tccacgttcg accccaggag
     2161 gggacgatga ccgcaccacg ctatgtcctg gcgcggcggc ccggaattca actcgacgtc
     2221 ttcgcaacac gctgcgggtt gcatcccgac atggtgcgcc gactggtcgc gctgggcctc
     2281 gtcgcttgcc agcaggatgc ccgcggcgat ctctggttcg aaccgtctgc gttggtgacg
     2341 gtcgcccgca tccagcggtt gaggaccggt ctgggactga actacgcggc gatcgggctg
     2401 gtgctcgacc tgctggaccg catcgaagag ctggaatccg cttctcggcg aaggaggaca
     2461 tcaccgtgga catcaacaag ctgacgcaga agtcgcagga ggcgttggcc gaagcgcaga
     2521 gcatcgcgac ccggatgggt catatcgagg tcgacggcga acatctgttg atggcgctga
     2581 tcgaccagcc cgagggtctc gtaccgcggc tgctggatca gacgggcgcc gacaccgcgg
     2641 ccctgcgcgc ggacctggaa cgcgagctga atcggcggcc gaaggtgagc ggcccgggcg
     2701 ccgcgccggg tcaggtgtct gtcacccggc gcctggcgaa cgttctggag gccgccgagc
     2761 gggaggccaa gcggctcaag gacgaatacg tgtcggtgga gcacctggtg atcgccctcg
     2821 ccgaagaagg cacatccacc gccgccggca ggatcttggc caaccacggc gtgacacgag
     2881 attcgttcct gagcgcgctg accaaggtgc gtggcaacca gcgggtcact tccgcgacgc
     2941 ccgagggttc ctacgaggcg ctggagaagt acggtcgaga tctggtcgcc gaagcgcagt
     3001 cgggcaagct cgacccggtg atcggccggg acgccgagat ccgcagggtg gttcagatcc
     3061 tgagccgcaa gaccaagaac aacccggttc tgatcggcga ccccggtgtg ggcaagacgg
     3121 cgatcgtcga ggggttggcc caacggatcg tgcgcggcga tgtcccggaa ggcttgcggg
     3181 acaagacgat cttctccctc gacatgggtt cgctggtggc cggtgcgaag taccgcggcg
     3241 agttcgagga gcggctgcag gcggtccttt cggaggtgaa ggcgggcgag ggccgaatcc
     3301 tgctgttcgt cgacgagttg cacaccgtcg tcggcgcggg cgcgacg
//
LOCUS       DASWBF010000556         5642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_389456,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000556 DASWBF010000000
VERSION     DASWBF010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389456"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VF874_20785"
     CDS             complement(<1..1286)
                     /locus_tag="VF874_20785"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9178404.1"
                     /translation="MLVGREPEQQRIAQLLAAARLGQSGVVVLLGEAGIGKTALLDDT
                     AAHAGDMVVMRTSGSERESALGFSGLHQLLRPALHLVDHIPGPQSEALAVALMLRHGP
                     APERFAVAAATLSLLSRYAEDSPLLMIVDDAHLLDPPSAETLRFVARRLLADPIALLM
                     SVRPEPDTPFTYSDLPVLALSGLDLASATALIAATGAAPATAEVVARLHRATFGNPLA
                     LVELSREIDRVTSLPPELPVPVPQTVAEAFGRRIEALPDATRLALLVAVVAEGDLAVT
                     SRAAEALGGRVEHLADAEAYGLLRLAGRAEFRHPLVRSAAYVAADPQARRAAHRAVAA
                     ALGPAAHDRRAWHLSEACIGPDDGVARDLVGVAERARVRGAHGIAAIALARSAELTTE
                     HSLRGSRLIAAGESAWLAGQVDRATDLLRQAAAWVS"
     gene            1491..2582
                     /locus_tag="VF874_20790"
     CDS             1491..2582
                     /locus_tag="VF874_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX9178405.1"
                     /translation="MTATTTAVPPAATVDQDKLMAFVFRAVEEAGAALNCALVVMGDR
                     LGYYRSLAHHGPSTSAELAERTGTDEHYAREWLNAQAAGSFVAYDPPTGRYHLPPEHA
                     VALTDEASPAFVGGLFQITHGTVRDAARILDAARTGAGVGWGEHNSDVHVGCERFFAP
                     TYTAHLISDWIPALEGVADKLTSGARAADVGCGHGASTILMGQAFPRSSFRGTDAHPE
                     SIATARERADASGAKAEVSFETAAADAFTGGPYDFVTMFDCLHDMGDPIGAARHIREL
                     IADDGTWMIVEPAASDRVEDNLNPIGRAYYGFSTLLCTPSSLAQPVGLALGTQAGPAR
                     VRDVVTAAGFTHFRLAARTPFNNVFEVRP"
     gene            2611..4746
                     /locus_tag="VF874_20795"
     CDS             2611..4746
                     /locus_tag="VF874_20795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9178406.1"
                     /translation="MSAAERRSRLPDTSGVVVRDGVSLHFDVYGNGPTTVLLLPAWSI
                     VDSRFWKAQVPYLARHYRVVTFDGRGSGRSDKPQGATAYADREFAADALAVLDATEIK
                     NSVLVGFSSGASWAVRVAAAHPDRVQGIFAIAPTCSLPAAHPERELALWLQTAECPDG
                     WLKYTRHHWLHGGLADFREFFFEQMYSEPHSTKQAEDMMRWSADIDAQTLADTTAGRL
                     DPEMLAAEPFEQIAKSVHCPVQVVHGTADRVRPWATGEQLAEVTGGSLTLIDGGGHGL
                     MARDPVRINTMIRDFIESVTSTPSRRARWTRAMRRPRRALYLSSPIGLGHARRDIAIA
                     TELRRARRGLEISWLAQNPVTRVLEAAGELVHPASRHLLCESAHIEYEAGEHDLHAFE
                     AIRRMDEILVANFMVFAEAVRDDPPDLVIADEAWEVDYFLHENPELKRFTFAWLTDFV
                     GWLPMPDGGPREAALTADYNAEMIEHRARFPRLRDRSIFIGNPQDVVAESFGAGLPEI
                     REWTEQNFEFSGYVVGSPPPSAPARAAVRRRLGLDPDHRLCVVTVGGTGVGGSLLRRV
                     LEAAPIIRKEMPELHFLAVTGPRIDPASMPRHRGVRIRGFVPDLADYLAACDMAVVQG
                     GLGTCMELTAAGTPFVYVPLAHHFEQNFHVRHRLDRYAAGRPMHYAEAADPDLLAKII
                     VDEMSTEPRFNPVETDGARRAAAMLAELL"
     gene            complement(4766..>5642)
                     /locus_tag="VF874_20800"
     CDS             complement(4766..>5642)
                     /locus_tag="VF874_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9178407.1"
                     /translation="VAPAMAGAVDLSALTPRATQGSDTARTAGAPAGVEITETNFEAE
                     VLARSNQVPVVVVLWSPRSDASIQLTETLAGLADTDAGKWSLATVNVDTAPRVAQIFG
                     VQAVPTVVAVAAGQPISSFEGVQPPEQLRRWVDSLMSATAGKLGGAADSEPEQVDPGL
                     AKARELLEEGNFDAALGAYKALLDYDPTHPEAKAAVRQISFLTRATKQRPDAPRAADA
                     APDDIEAAFTAADVEILNQQVDAAFNRLIDLVRRTSGDDRTKVRTRLIELFDLFDPAD
                     PAVIAGRRNLANALY"
BASE COUNT          911 a         1925 c         1903 g          903 t
ORIGIN      
        1 tccgacaccc atgccgctgc ctgtcgcagg aggtcggtgg cacggtcgac ctgccctgcg
       61 agccaggccg attcgccggc ggcgatcagt cgcgagcctc gcagcgagtg ctctgtggtc
      121 agttccgcgc tgcgggccaa cgcgatcgcg gcgatgccgt gcgcgcctcg gacccgggcc
      181 cgctctgcga ccccgaccag gtcgcgcgcc accccgtcgt cggggccgat gcaggcctcg
      241 ctcaggtgcc atgcccgacg gtcatgcgcc gcgggaccca gcgcggcggc gacggcgcga
      301 tgcgccgccc tccgcgcctg cggatccgcc gcgacgtagg cggccgaccg gaccaacggg
      361 tggcggaact cggcccgtcc ggcgagccga agcaatccat acgcctccgc atcggcgagg
      421 tgctcgactc ggccgcctag cgcctcggcg gcccgcgagg tcacggccaa gtcgccctcg
      481 gcgacgaccg cgaccagaag ggccagtcgc gtcgcgtcgg gcagtgcttc gatccgccgc
      541 ccgaacgctt cagccacggt ctgcggaacc ggcaccggca gctctggtgg caagctggtg
      601 acgcgatcga tctcgcggga caactcgacg agagcgagcg gattgccgaa cgtggcacgg
      661 tgcaaccggg caacgacttc ggcggtggcg ggcgccgcgc cggttgccgc aatcagcgcc
      721 gtggccgacg ccaggtccag tccgctcagt gccaacacag gcaggtccga gtacgtgaac
      781 ggcgtatcgg gttcgggccg taccgacatc agcagcgcga ttgggtcagc gagcagccgc
      841 cgcgccacga accgcagcgt ttctgccgac ggcggatcca gaaggtgtgc gtcatcgacg
      901 atcatgagca ggggggagtc ttccgcgtac cggctcaaca agctcagggt cgcagcggcc
      961 accgcgaacc gctcaggcgc agggccgtgc cgcaacatca gtgcgaccgc cagcgcctcg
     1021 gattgcgggc cggggatgtg atcgacgaga tgcaaagcgg gccgcaacaa ttgatgcaaa
     1081 ccagagaagc cgagcgccga ctcacgctcg ctgcccgacg tccgcatcac gaccatgtcg
     1141 cccgcgtgcg cggccgtgtc gtcaagcagt gcggtcttgc cgatcccggc ctccccgagc
     1201 agcaccacca cgccgctttg tccgagccgc gcggcggcaa gcagctgagc gatgcgttgc
     1261 tgttcgggtt cgcgacccac cagcatgcgc tgatcgtacg gtgagcacgc gcaccgtggc
     1321 cggatgctgc ttcatttcca gcggggactt tgtgaacggg acgttccagg cacaccgcga
     1381 acggcgtgcc ccggtcgcag gtcgacaaga gactaggggg ggtcacctga tttgttcgcg
     1441 gtcatcgcgg tgccacggtg gtgttcgtcg agacagaaag gactacttcg atgaccgcga
     1501 caaccactgc cgtcccgccc gctgcgacgg tcgaccaaga caagctcatg gccttcgtgt
     1561 tccgcgcggt tgaggaggcc ggcgctgcgc tgaactgcgc gctggtcgtg atgggtgacc
     1621 ggctcgggta ttaccggtca ctggctcatc acggacccag cacttcagcc gagctggccg
     1681 aacgcaccgg caccgacgaa cactacgccc gggaatggct caacgcccag gccgcgggat
     1741 ccttcgtggc atatgacccg ccgaccgggc gctaccacct gccacccgag cacgcagtgg
     1801 cgctcaccga cgaagccagt ccggccttcg tcggcggcct gttccagatc acccacggaa
     1861 ccgtccgcga cgcggcccgc atcctcgacg ccgcgcgcac cggcgccggc gtcgggtggg
     1921 gcgagcacaa ctccgacgtg cacgtgggct gtgagcgatt cttcgcgccg acgtacaccg
     1981 cacacctgat ctccgactgg attccagcgc tggagggcgt ggcggacaag ctcacgtccg
     2041 gtgcccgggc agccgatgtg ggctgtggcc acggcgcctc gacaatcctt atgggacagg
     2101 cgtttccacg ctccagcttc cggggcaccg atgcccaccc cgagtccatc gccacggctc
     2161 gggaacgcgc agacgcttcc ggagccaagg cagaggtcag ttttgagacc gcggcggccg
     2221 acgcgttcac cggcggcccc tatgacttcg tcacgatgtt cgactgcctg cacgacatgg
     2281 gtgacccgat cggtgcggcg cgccacatcc gcgagctcat cgccgatgac ggaacgtgga
     2341 tgatcgtgga gccggcggcc agcgaccggg tggaagacaa cctcaacccc atcggccggg
     2401 cctactacgg gttctccacg ttgctgtgca cgccgtcgtc gctggcgcag ccggtcgggc
     2461 tcgcgctcgg cacgcaggcc ggaccggccc gcgtccgaga cgtggtgacg gcggcgggtt
     2521 tcacccactt ccggctggcc gcgcggaccc cgttcaacaa cgttttcgag gttcggccct
     2581 gaaccacacc agaaggacca cgatagatac atgagcgccg cagagcgtcg atcccgccta
     2641 ccggacacgt cgggtgtagt ggttcgcgac ggagtttcac tgcacttcga cgtgtacggt
     2701 aacggcccga caacggttct cctgctgccc gcctggtcga tcgtcgattc gcggttctgg
     2761 aaggcgcagg tgccctacct ggcgcgccac taccgggtgg tgacgttcga cggccgcggc
     2821 tcggggcggt ccgacaagcc acagggcgcc accgcctacg ccgaccgtga gttcgcggcc
     2881 gacgcattgg cggtgctgga tgcgacggag atcaaaaaca gtgtgcttgt cggcttttcg
     2941 tccggggcaa gttgggcggt ccgggtcgcc gccgcgcacc cggatcgagt gcaaggcatt
     3001 ttcgccatcg ctccgacgtg cagcctgccg gcggcccatc ccgagcgcga gctcgcgctg
     3061 tggctgcaga ccgccgaatg ccccgacggt tggttgaagt acacccggca tcactggctg
     3121 cacggcggct tggccgactt ccgggagttc ttcttcgagc agatgtactc cgagccgcat
     3181 tcgaccaagc aggccgaaga catgatgcga tggtcggccg atatcgacgc gcagaccttg
     3241 gcggacacca cagcagggcg tcttgacccg gagatgcttg ccgccgagcc attcgaacag
     3301 atcgccaagt ctgtgcactg cccggtacaa gtcgtgcacg gaacggccga tcgagtgcgg
     3361 ccgtgggcca ccggagaaca gctggccgaa gtcaccggcg ggtccttgac gctgatcgac
     3421 ggcggcggcc acgggttgat ggcacgcgat cccgtgcgga tcaacacgat gattcgcgat
     3481 ttcatcgagt cggtgacttc gacgccgtcc aggcgcgctc ggtggacccg cgcaatgcgg
     3541 cgcccgcggc gggcgctgta tctgtcgtcg ccgatcggtc taggccatgc ccgccgtgat
     3601 attgcaatcg cgaccgaatt gcgaagagcg cggcgaggtt tggaaatctc ttggttggct
     3661 cagaatccgg tgacccgggt gttggaggct gccggtgagc ttgtgcatcc ggcgtcgcgg
     3721 catctgctct gcgaatccgc acacatcgaa tacgaagcgg gcgaacacga cctgcacgcg
     3781 ttcgaagcga ttcgcaggat ggatgagatt ctggtcgcca acttcatggt gttcgccgag
     3841 gcggtccgcg acgatccacc ggacctggtg atcgccgacg aggcctggga agtcgactac
     3901 ttcctgcacg agaacccaga gctcaagcgg ttcacgttcg cctggctcac cgacttcgtc
     3961 ggctggttgc caatgcccga cggcgggcca cgcgaagccg cactcaccgc ggattacaac
     4021 gcggagatga tcgaacatcg ggcgaggttc ccgcgcctgc gcgaccggtc gatcttcatc
     4081 ggcaacccgc aagacgtggt tgctgagtcg ttcggcgccg ggcttcccga gatccgcgag
     4141 tggactgaac agaacttcga gttctcgggc tacgtcgtgg gttctccgcc cccgtcggcg
     4201 ccggcgcggg cggcagtgcg tcgcaggctc ggcttggatc ccgatcaccg gttgtgcgtt
     4261 gtcaccgtcg gtgggacggg cgttggtgga tctctgttgc ggcgagtgct ggaggcggcg
     4321 ccgatcatcc gcaaagagat gccggaactt catttcctgg ccgtcaccgg accacgcatc
     4381 gaccccgcat cgatgccccg ccaccgcggg gtgcggattc gaggcttcgt acctgatcta
     4441 gccgactacc tcgccgcatg tgacatggcc gtcgtccagg gcgggctggg gacgtgcatg
     4501 gagctcaccg ccgccggcac cccgttcgtc tacgtgccgc tagcgcatca cttcgagcag
     4561 aacttccacg tccggcaccg actggaccgc tacgccgccg ggcggcccat gcactatgcg
     4621 gaagctgccg accctgatct gctcgccaag atcatcgttg acgagatgtc caccgagccg
     4681 cggttcaacc cagtcgagac cgacggagcg cggcgggcag cggccatgct ggccgagttg
     4741 ttgtgaccag gtcactgctg cacgatcagt acagcgcgtt ggcgaggttt cgccggcccg
     4801 cgatgaccgc cgggtcggcc gggtcgaaca ggtcgaacag ctcgatcaac cgcgtccgca
     4861 ccttcgtgcg gtcgtcaccg gacgtgcggc ggaccaagtc gatcagccgg ttgaacgccg
     4921 cgtcaacctg ctggttgagg atctcgacgt cagcggccgt gaacgccgct tcgatgtcgt
     4981 caggcgcggc gtcggccgcc cggggggcgt ccgggcgctg cttggtcgcg cgcgtcagga
     5041 acgagatctg acggaccgcg gccttcgctt cagggtgcgt cgggtcgtag tccagaagtg
     5101 ctttgtacgc cccgagcgcc gcgtcgaaat tgccctcttc gagcagctcg cgcgccttcg
     5161 ccaacccagg atcgacctgt tcgggttccg aatcggctgc accgccgagc tttccggcag
     5221 tagccgacat cagcgagtca acccaccggc gcaattgttc gggcggctgg acgccctcga
     5281 agctggagat cggctgcccg gcagccaccg cgacgacggt cggcacggct tgcacgccga
     5341 agatctgagc gacccgcggc gcggtgtcga cgttcacggt cgccaacgac cacttgccgg
     5401 catcggtgtc cgccagaccc gccaacgttt cggtgagttg gatggacgcg tcgctgcgcg
     5461 gcgaccacag caccaccacc accggcacct ggttggaccg ggccagcacc tcggcttcga
     5521 agttcgtctc ggtgatctcg acgccggccg gcgcgccggc ggtgcgggct gtgtcgctgc
     5581 cctgcgtggc gcgcggcgtg agcgcggaaa ggtcaacggc gcccgccatg gcgggcgcga
     5641 cc
//
LOCUS       DASWBF010000557         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_644947,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000557 DASWBF010000000
VERSION     DASWBF010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644947"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VF874_20805"
     CDS             complement(<1..348)
                     /locus_tag="VF874_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178408.1"
                     /translation="MNKFVVLGFSLVIAVEVLALVMVNHRFVLALSGVAAALVLLEGR
                     RLLTRSLATDQPEPVSGGTAESMRRWISRTESMIRWADSSRADWDRHLRPRLAREFML
                     ATGQRMGRDPAALE"
     gene            complement(345..1340)
                     /locus_tag="VF874_20810"
     CDS             complement(345..1340)
                     /locus_tag="VF874_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX9178409.1"
                     /translation="MLLLFAQALIDVPRWIMMPVTDRATGRVVALIALVVVVGIALRG
                     YLPGEDQAGDQPTDNTAAMIGTVTLLGAAVVIIMIAIVATLRQPPTPAPAGQPLPRRP
                     SGERWHLRWRIVLIGLGLVMVWLLVVVLLMRLSVRLDAQFQPTAPSTATPGATPTPAP
                     PPPPPAPPETEPALFWYLFATTVFMLVFLAVATVINVRRHRRPPTPKPVGEDVAAPDD
                     DEPAPPPLAVAAERGLAEMGDLSREPREAIIACYAAMEDALATAPGAVPQESDTPTEV
                     LARAVEHHAIHAGTATDLVELFAEARFSPHVMNEGHRETAVRALQLVLAELKSVA"
     gene            1364..2470
                     /gene="cobG"
                     /locus_tag="VF874_20815"
     CDS             1364..2470
                     /gene="cobG"
                     /locus_tag="VF874_20815"
                     /EC_number="1.14.13.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0019250 - aerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HEX9178410.1"
                     /translation="MFRTRDVDACPGALSLHRAADGSLARIRLPGGAITPAQLQALAR
                     AAKAFGSPDIELTSRGNIQIRAITDEAAVAQAVAAAGLLPSATHEKVRNIVASPLSGR
                     VGDHPDVRAWVAELDAAIQASPVLASVGGRFWFGIDDGRGDVSGLAPDVGVHVLGDSV
                     ALLLAGRDTGVRLPCAETVTTLVAVAARFTAVRGKAWRVSELDGVSPLLDALPVSAER
                     GATWPPAMRPPVGWLVQNDGRVALGAAVPLGVLAAGTAESLAAIEAPMVVTPWRSVLV
                     CDLDEAVADMALRVLAPTGLVFDENSPWLSVSACTGSPGCEHSRADVRADAANALEFG
                     GAHRHYVGCERACGSRPGDDVLVATGDGYRRLPS"
     gene            2481..3101
                     /locus_tag="VF874_20820"
     CDS             2481..3101
                     /locus_tag="VF874_20820"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX9178411.1"
                     /translation="MLDYIRDPAEIYRQSFATIRAEADLSRFPDDVTDVVVRMIHTCG
                     QVDLAEHIAFTDDVVARVRDALAAGAPVLCDSSMVAAGVTQLGGGNDVVSLVADPRAA
                     DLAARLGTTRSAAAVELWADRIGGAVMAIGNAPTAVFRLLELVDDGVAAPAAVLGGPV
                     GFVGSAQCKEELVARPRGIAYLLVTGRRGGSAMAAAAVNAMAGGPV"
     gene            3098..>3277
                     /locus_tag="VF874_20825"
     CDS             3098..>3277
                     /locus_tag="VF874_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9178412.1"
                     /translation="MTGTLWGVGLGPGDPELVTVKAARVISEADVVAYHSARHGRSIA
                     RGIAEPYLRAGQIEEH"
BASE COUNT          455 a         1105 c         1210 g          507 t
ORIGIN      
        1 ctccagcgcc gcaggatccc tacccatccg ctgaccggtc gcgagcatga attcccttgc
       61 gagtcttggc cgtaagtgcc gatcccagtc ggcacgactg gagtcggccc agcggatcat
      121 cgactcggtg cgagagatcc agcgccgcat cgactcggcg gtaccgccgg acacgggctc
      181 gggctggtcc gtcgccagtg agcgggtcag tagccggcgg ccttccagca acaccagtgc
      241 ggccgcgacg cccgaaagcg caagcacgaa gcggtgattc accatcacca acgccagcac
      301 ctccaccgcg atcaccaggc tgaagccgag gacgacgaac ttgttcatgc cacgctcttc
      361 agttccgcga ggaccagttg gagcgcgcgc accgcggtct cgcgatgccc ttcgttcatg
      421 acgtgcgggc tgaaccgggc ctcggcgaac agctcgacga ggtcggtggc ggtgccggca
      481 tggatggcat ggtgttcgac cgcgcgggcc agcacctccg tcggggtgtc cgactcctgc
      541 ggcacagcgc caggcgctgt ggcgagcgcg tcctccatcg cggcgtaaca ggcgatgatc
      601 gcctcgcgcg gttcgcggct caaatcaccc atctcggcca ggccccgttc ggccgcgacc
      661 gcgagcggcg gcggcgccgg ctcgtcatcg tcgggcgcgg caacatcctc gccgaccggc
      721 ttcggagtgg gtggccgccg gtgccgtcgc acgttgatca ccgtggccac ggccaggaat
      781 accagcatga acaccgtcgt cgcgaacagg taccagaaca gggcgggttc ggtctccggc
      841 ggcgcgggcg gcggaggtgg cggggccggt gtcggggtgg caccgggtgt agcggtgctt
      901 ggcgccgtcg gttggaactg tgcgtcgagg cgcacgctca atcgcatcag cagcacgacg
      961 accagcagcc acaccatgac caagccgagc ccgatcagca cgattcgcca cctcagatgc
     1021 cagcgctcac cgctgggtct tctcggcagt ggctgaccgg ccggcgcagg tgtcggtggt
     1081 tgtcgcagcg ttgcgacgat cgcgatcatg atgatgacga ccgcagcacc gagcagcgtg
     1141 acggtgccga tcatggctgc cgtgttgtcg gtgggctgat cgcctgcctg gtcctcgccg
     1201 ggcaggtatc cgcgcagcgc gatgccgacg accaccacca gcgcgatcag ggcgaccacc
     1261 cgccctgtcg cccgatcggt aacaggcatc atgatccatc gtggcacgtc gatgagcgct
     1321 tgcgcgaaga gcaacagcat ctgcagtcgc ctacggtggt gggatgttcc gcacgcgtga
     1381 cgtcgacgcc tgccctggcg cgctgtcgct gcatcgcgcg gcggacgggt cgctggcccg
     1441 gatcaggctg ccgggcggag cgatcacccc ggcgcaactg caggcgctgg cccgagcggc
     1501 gaaagcgttc ggctcccccg acatcgagct cacctcgcgc ggcaacatcc agatccgcgc
     1561 catcaccgac gaagcggccg tcgcgcaggc ggtggccgcg gccggtctgc tgccgtcggc
     1621 cacccacgag aaggtgcgca acatcgtcgc ctctcccctg tcggggcggg tgggcgatca
     1681 ccctgatgtg cgcgcgtggg tggccgagct cgacgcggcg attcaggcgt cgccggtctt
     1741 ggcttcggtc ggcggccggt tctggttcgg catcgacgac gggcgcggcg acgtgtcggg
     1801 gctggccccc gacgtcgggg tgcatgtgct cggtgattct gtggcgctgc tgctggccgg
     1861 gcgcgacacc ggcgtccggc tgccgtgcgc cgagacggtg acgacgttgg tcgcggtggc
     1921 cgctaggttc acagcggtgc gcggaaaggc ttggcgggta agcgaattgg acggtgtcag
     1981 cccgttgctc gacgctctgc cggtgtcggc cgagcgagga gcgacctggc cgccggcgat
     2041 gaggccgccg gtcggctggc tggtgcagaa cgacggccga gtggcgttgg gcgcggcggt
     2101 tccactcggc gtgctggcag ccggcacggc tgaatcccta gcggcgatcg aggcgccgat
     2161 ggtggtcacg ccgtggcgct ccgtgctggt gtgcgacctc gacgaagcag tcgcggacat
     2221 ggcgcttcgg gtgttggcgc cgacgggcct ggtgttcgac gagaactcgc cgtggctttc
     2281 cgtcagcgcg tgcaccggta gccccggctg cgagcactcc cgcgccgacg tgcgcgccga
     2341 cgctgcgaac gctttggagt tcggcggcgc gcatcggcac tacgtggggt gcgagcgggc
     2401 ctgcggcagc cggcccggcg acgacgtgct ggtggccacc ggtgacggat accgccgcct
     2461 gccttcgtag ggtgagcggc gtgctcgact acatccgcga tccggccgag atataccggc
     2521 agtcgttcgc gacgattcgc gccgaggccg acctgtcgcg gtttcccgac gacgtcacgg
     2581 atgtcgtggt gcggatgatc cacacctgcg ggcaggtcga tctcgccgag cacatcgcgt
     2641 tcaccgatga cgtcgtcgcc cgggttcgcg acgcgctggc cgccggtgcg ccggttcttt
     2701 gtgactcgtc gatggtggcc gccggcgtca cgcagctcgg tggcggaaac gacgtggtgt
     2761 cgctggtcgc cgatccacgc gccgcagacc tggccgctcg cttgggcacc acccggtcgg
     2821 cggcggcggt cgagctgtgg gcggaccgca tcggcggcgc ggtgatggcg atcggcaacg
     2881 ccccgaccgc ggtgtttcga ctactcgaac tggtcgacga cggggtggcc gcgccagccg
     2941 ccgtactcgg cgggccggtc gggttcgtcg gctcggcgca gtgcaaagag gagctcgtcg
     3001 cccgcccgcg cggcatcgcg tatctgctgg tgacaggacg gcggggcggc agtgcgatgg
     3061 ctgccgccgc ggtgaacgcg atggccggcg gcccggtgtg acgggcacgc tgtggggcgt
     3121 cgggctgggg cccggcgatc ccgagctcgt caccgtcaag gccgcccgcg tcatcagcga
     3181 ggccgacgtg gtcgcgtatc acagcgcgcg gcacggccgc agcatcgcgc gcggcatcgc
     3241 cgagccgtac cttcgggcgg gccagatcga ggagcac
//
LOCUS       DASWBF010000558         7389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_183173,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000558 DASWBF010000000
VERSION     DASWBF010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183173"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VF874_20830"
     CDS             complement(<1..230)
                     /locus_tag="VF874_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178413.1"
                     /translation="MRSVSGFVSSFHLVKAIASHLAGFGRFLACLDPPVTDLAVLDRR
                     THIEAWLAVLAAARHDDGTAMSVGHQRGQILTV"
     gene            complement(243..1004)
                     /locus_tag="VF874_20835"
     CDS             complement(243..1004)
                     /locus_tag="VF874_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178414.1"
                     /translation="MDHVAKRLEEKLVTENYNLDAFEASIATDLLGDLIRNLPYIADN
                     KRTLDIAHRNLLTFVGIDVDDPATWPQNIAFCDQIFDHICTAASELHSLIAPAGFYKD
                     VYGIDHANLIRLREADHALLAKGEPHTGTPVLCCSAHRQGVVVGRNTQTVTVRHCGFL
                     EDKYDLLERNYCLQAIKPLVGDYKRIEESSDVVFFDWKKYKRQGTVVETLGPLIKVSY
                     FLKNGKERACWIEPTRLVETESSETIPAIAVNLDS"
     gene            1217..1375
                     /locus_tag="VF874_20840"
     CDS             1217..1375
                     /locus_tag="VF874_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178415.1"
                     /translation="MSGLDLDCPFTFGGLGLGDLGAAAPLGKLSFDPISFRAQLRRWR
                     LNCGVNVA"
     gene            complement(1400..3313)
                     /locus_tag="VF874_20845"
     CDS             complement(1400..3313)
                     /locus_tag="VF874_20845"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX9178416.1"
                     /translation="MRGLVRGRRHTGTRVRYRSAGAGGAAWRRRRGGGGGGHQGVESH
                     RGRGQCPAVGCDRGADSRRCDREGERASWACDGWDSAAGEVAAALGTSPKKASSQMQL
                     GVTLRHQLRQVAALYLDGRVSYRVISAINWHTHLVDDDEPLAQIDTALAKRAAAWGEL
                     SDYKLEQAIDFWVRRHDPDALRRTRVSARSRDLYVGNKNDESDTTAVWGRLYATDAPA
                     LHRRLMDMAHGVCDDDPRTIAQRRADALGALAAGAERLACGCANPHCPASGTDGRAAT
                     VVIHVVTDAAALDGQPDPQMSGEESPPPDPPIEGKLRPPAGLLLRGGIVPTPLLAELI
                     RAGATISHVRPPGDAPESGYLPSAKLAEFVRVRNLTCRFPYCEQPAEFCDLDTIPPAR
                     ADPPVQAGVRRTGGSCLGVGDAWEEFLTGLGERGLACPLLVISDGTAGLVGAIERTMG
                     AALRQRCLMHRARINVLAKVPKNAHADMKADYWAIFDLPESVAPGLEAVAIAQQRIDA
                     FAQRWRDSYPAAVRCLLADRDSLTVYLRFPREHWTRVRHSNFERTFGETRRRVKVIGR
                     LPGEHCCLKLVWAVLDRASVGWRGFAITPAGQRLLQDLRRALHDPPTQLRQPDSVSHT
                     ETATTPPDSAAIA"
     gene            complement(3360..5651)
                     /locus_tag="VF874_20850"
     CDS             complement(3360..5651)
                     /locus_tag="VF874_20850"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9178417.1"
                     /translation="MRRTVIRVLLVTTIAALVAGGVAWLAGARDIADILWAAGTLVAI
                     VPAVWWVVAALRRGHLGADIIAVLSLVGTLAVHEYLAGALIGVMLATGQALESAAERR
                     AAKDLRSLLERAPRVARRRTGDDLETVPVDEVVVGDVLVVGPGEMLPVDARIASEWAV
                     LDESVLTGESVHIEYRSGEAVRSGAVNAGAAVEIDTMAPAAESTYAGIVRLAQQAAAE
                     SAPVVRLADRVAAWFLPLALAVAGFAWLISESAERAVAVLVVATPCPLLLAAPVAIVS
                     GLSRASRIGVVVRDGGALENLGRATTLVLDKTGTVTTGRPRGTDVLVAPEWTSTELLR
                     LAASADQVSPHVMARAIVEEAATQRLTLSMPSDVVEEAGRGVIATVGGRRIAVGTREL
                     PADSPQWAMAAEKRAAFDGAVIAWVSVDERLAGAIVFNDPLRQDAPRTMRRLRAAGIT
                     RLVMLTGDRPAPAQQIGAVLGLDEVRAQQTPADKVAGVRAEREKAVTAMVGDGVNDAP
                     ALAAAHVGIAIGVHGSTASSEAADIVLTTDRLDRLADAMVIARRSRRIAVQSAVIGMA
                     LSLVAMGFAAFGFLPPAVGALLQEGIDVAVILNALRALGGRDPTAPAIDVSTKGLIQR
                     FSAEHDAMRRDLSVLRDIGHLLTSGQRAQALALLDRADDFLRDTLLPHEHAEDRMLYP
                     ALARPLGSAEATATMSRMHGEIDRLSGRLHAHREAADAAGGITDDQTDDLLACLYGLH
                     ALLSLHFSVEEESYFALLPPAAD"
     gene            complement(5662..>7389)
                     /locus_tag="VF874_20855"
     CDS             complement(5662..>7389)
                     /locus_tag="VF874_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9178418.1"
                     /translation="TGSRRLARYKVLVKRLVWSASKDLGDVDVLMTDKTGTLTEGRIT
                     LIDAVDADGAHDDSVRRIGLLATDVDRESGGASADPLDAALWESPASQRAAAGTTRRL
                     AVRPFDHIRRSMSALVDDGDRRLLIVKGAREQVLAQCVDVPAGSQNTLAALFADGRRV
                     VAVAGKTASELTEITSEDECGLALRGFLVFADEPKATARDSLTQLAGLGVEVKIATGD
                     NPQVAEKVCAEIGLASKGTITSVEMEGFDDDGFVRAARQNTIFARITPEQKGRLVSAA
                     RRSGRSVGFLGDGVNDALALHSADVGISVDTATDVAKDAADVVLLEKDLGVLATGIAE
                     GRRIFANTIKYVLMGTSSNFGNMFSPAAASAVLPFLPMLPSQILLNNLLYDTSQLAIP
                     SDHVDEEQLHAPSHWNIAFIRRFMLTFGPISSLFDFMTFGLLLGVLHAGAVEFRTGWF
                     LESLATQTLIIFAIRTRRVPFLKSRPGLLLTISTLTVIAVGVLLTVSPWAHNLGFTPL
                     PWQFFAALAGMTIAYLVLVEITKTLFYVEPMRLAGQPHRTRGDRHRIHRRAARFSHAG
                     RIQSPAPQG"
BASE COUNT         1282 a         2461 c         2399 g         1247 t
ORIGIN      
        1 acggtgagga tctgcccgcg ttggtgtccc accgacatcg cggtgccgtc gtcgtgccgg
       61 gcggcggcca gcacggccag ccacgcctcg atgtgggtgc gccggtccag taccgccagg
      121 tcggtgacgg gcgggtccag gcaggccagg aagcgaccga aaccggcgag gtggctggcg
      181 atcgccttga ccaggtgaaa cgacgaaacg aacccagaaa cactacgcac cactcgatag
      241 acttaggaat cgaggttaac agcaattgca ggaattgttt cggacgattc agtttcaact
      301 agccgcgtcg gctctatcca acaggcacgt tcttttccat ttttcaaaaa gtaactcacc
      361 ttaatgaggg gcccaagtgt ttctacaacg gtgccctgtc ttttgtattt cttccagtcg
      421 aagaaaacaa catcagagga ctcctcgata cgtttgtagt cacccaccaa cggctttatc
      481 gcttgaaggc agtagttcct ttccagtaaa tcatatttgt cttccaggaa cccgcagtgc
      541 cgcaccgtga cggtttgcgt gtttctaccc actacgaccc cctgacgatg cgctgaacag
      601 catagaacgg gagttcctgt atgcggctcg cccttggcca aaagcgcgtg gtcagcctct
      661 ctaaggcgaa ttaaattcgc gtggtctata ccgtagacat ctttataaaa accggctggc
      721 gctatcaagc tatgcaattc acttgcagct gtgcagatgt ggtcgaaaat ttggtcacag
      781 aatgcgatgt tttgcggcca tgtcgcggga tcgtccacat cgatacccac aaacgttagt
      841 aggttgcgat gcgcgatgtc gagggtgcgc ttattatcgg caatgtatgg caggtttctt
      901 atcagatcgc ccagtaagtc cgtggcgatc gaagcctcga aagcatccaa attataattt
      961 tcggtaacga gtttttcctc tagccgcttc gcaacatgat ccatgttgtc aaggatcgga
     1021 tatgcggtcc tgtcccgcaa tacgggccca ctgccgccaa ggctcagtgg ggatggtgca
     1081 ctacgtgctc aatgacgcta gaaacccagc aggcgaccaa gaccgaatag accgccaggc
     1141 cgtgatacaa gctgcgtcac cggttgtaca ggttgtgcag acggtgttgg tgtgggtgct
     1201 gcttcgagca tgcgcagtgt ctgggctgga cttggattgt ccgttcacgt tcggcggcct
     1261 gggcctcggc gacctcggcg cggcggcgcc actcggcaag ctcagcttcg acccgatttc
     1321 gttccgcgcg cagttgcgac ggtggcgtct caattgcggt gtaaatgtgg cctgatgtga
     1381 gttccgctcc ttggtgatgt tatgcgatgg ctgccgaatc gggtggtgtg gttgcggttt
     1441 cggtgtggga taccgaatcc ggttggcgga gttgggttgg tgggtcgtgc agggcgcggc
     1501 gcaagtcttg tagcaggcgt tgtccggcgg gggtgatggc gaatccacgc cagccgactg
     1561 aggcgcgatc cagcacggcc cacaccagct tcaggcagca gtgctcgccg ggcagccgac
     1621 cgatcacctt gacccgacgg cgggtctcac cgaaggtacg ttcgaagttc gagtgccgca
     1681 ccctggtcca gtgctcacgc gggaaccgca ggtacaccgt cagcgagtcg cggtcggcga
     1741 gcagacagcg caccgctgcc ggatacgagt cgcgccaacg ttgggcgaac gcgtcgatgc
     1801 gctgctgagc gatggccacc gcctcgagcc cgggcgcgac actctcgggc agatcgaaga
     1861 tggcccagta gtcggccttc atgtcggcgt gggcgttctt gggcaccttg gctaagacgt
     1921 taatgcgggc gcggtgcatc agacagcgct ggcgcagcgc tgcgcccatg gtgcgctcga
     1981 tggcgcccac aagtccggcc gtgccgtcgg agatgaccag cagcgggcaa gccagcccgc
     2041 gctcacccag cccggtcagg aactcttccc acgcgtcgcc gactccgagg cagctgcctc
     2101 cagtccgacg aacaccggct tggacgggtg ggtcggcccg agcggggggg atcgtgtcga
     2161 ggtcgcagaa ctccgccggc tgctcgcagt aggggaagcg gcaggtcaga ttccgcaccc
     2221 ggacgaactc cgccagcttg gccgacggca gatagcccga ttccggtgcg tcgccaggag
     2281 ggcggacgtg gctgattgtg gcgccggcgc ggatcagctc ggccaaaagc ggtgtcggca
     2341 cgatgccgcc gcgcaatagc agcccggccg gcggcctgag cttgccctcg atcggcggat
     2401 cgggcggggg tgattcctca ccagacatct gcgggtcggg ctggccgtcg agcgcggcgg
     2461 cgtcggtgac cacgtggatg accacagtgg ctgcccgccc gtcggtgccg ctggcagggc
     2521 aatgcgggtt ggcgcaaccg caggccagcc gctcggcacc ggcggcgagc gcgcccaggg
     2581 catcggcacg gcgttgggcg atggtgcgcg ggtcgtcatc gcacacgccg tgtgccatat
     2641 ccatcagccg gcgatgcagt gcgggggcgt cggtggcata cagccgtccc cacaccgccg
     2701 tggtgtcgga ttcgtcgttt ttattgccga catagaggtc tcggctgcgc gcgctgaccc
     2761 gggtgcggcg cagcgcatcg gggtcgtggc ggcgcaccca gaagtcgatg gcctgctcga
     2821 gcttgtaatc agacagctca ccccaggccg cggcccgctt ggccagcgcg gtgtcgatct
     2881 gcgccagcgg ctcgtcgtcg tcgacgaggt gggtgtgcca gttgatcgcc gagatcaccc
     2941 ggtagctgac cctgccgtcc agatacagcg ccgcgacctg gcgcagctgg tggcgcagcg
     3001 tcacgccgag ctgcatctga ctggaggcct tcttcgggct ggtgccgagc gcggcggcga
     3061 cttcgccggc cgccgaatcc cacccgtcac aggcccacga cgcccgctcg ccctccctat
     3121 cgcagcgccg gctgtcagct ccgcgatcgc atccaaccgc cgggcattgg ccgcggcctc
     3181 gatgcgattc cactccttga tggccgccac caccaccgcc tcgtcgtcgg cgccacgcag
     3241 cgcctccggc tccggcggaa cgatatcgaa cacgtgttcc agtatgccgc cgccccctga
     3301 caagtccccg cactcattcg cggctccatc ggagctgagc ggtcagaagt aaatgcgagc
     3361 tagtcggccg caggcggaag caaggcgaaa tagctctcct cttcgacaga gaagtgcaaa
     3421 gacagcagcg cgtgaagccc gtagagacag gccagcaggt cgtcagtctg gtcgtcggtg
     3481 atgccaccgg cggcgtcggc ggcctccctg tgggcatgca gccttcctga gaggcggtca
     3541 atctcgccgt gcatccggct catcgtcgcg gtggcctcgg cgctgcctag cggacgcgcg
     3601 agcgccgggt acagcatgcg gtcctcggca tgctcatgcg gaagcaacgt gtcacgcagg
     3661 aaatcgtcgg cgcggtccag cagggccagg gcctgcgcac gctgcccact tgtcagcagg
     3721 tgcccgatgt cgcgcagcac cgacagatcc cgtcgcatcg cgtcgtgctc ggccgaaaaa
     3781 cgttggatca gacctttggt tgaaacgtcg atggcgggcg ccgtcgggtc gcggccaccc
     3841 agtgcgcgca acgcgttgag gatcactgca acgtcgatcc cttcttgcag taacgcaccg
     3901 accgccggcg gcaggaaccc gaacgccgcg aatcccatcg cgaccaggga cagggccatg
     3961 ccaatcaccg cgctctgcac ggcgatccgt cgcgagcgac gcgcgatcac catcgcgtcg
     4021 gcgagccggt cgagccggtc ggtggtgagg acgatgtccg cggcctcgga actggcggtg
     4081 gagccgtgaa cgccgatcgc gatgccgacg tgtgcggccg ccagcgccgg ggcgtcgttg
     4141 acaccgtcgc cgaccattgc ggtcaccgcc ttctcccgct cggcgcgcac gccggcgacc
     4201 ttgtcggctg gagtctgctg ggcgcggacc tcgtcgaggc cgagcacggc gccgatctgt
     4261 tgcgcgggcg ccggacgatc accggtcaac atcaccagac gcgtaatgcc cgctgctcgc
     4321 agtcggcgca tcgtacgggg cgcgtcctgc cgcagcggat cgttgaaaac tattgcacca
     4381 gcaaggcgtt cgtcgacact cacccacgcg atcacggcac cgtcgaacgc ggcgcgcttt
     4441 tcggccgcca tagcccactg cggtgaatcc gccggaagct cgcgggtgcc caccgcgatg
     4501 cgtcgaccgc cgaccgtcgc gattactccc ctgcccgcct cctccacgac gtcggagggc
     4561 atgctcagcg taagccgttg agtggcggct tcttccacga tggctctggc catcacatgc
     4621 ggcgagactt ggtctgccga cgccgcaagc cgcagcaact ctgtcgacgt ccattccgga
     4681 gcgaccagca cgtcggtgcc acgaggacgg ccggtggtca ccgtgcccgt cttgtccagc
     4741 accagagtgg ttgcccggcc gaggttttcg agggcgccgc catccctgac gacgacgccg
     4801 atccgggacg ctcgcgacag tcccgaaacg atcgccaccg gcgcagccag tagcagcggg
     4861 catggtgtcg cgaccacgag gacagcgacg gcacgctcgg ccgattcgct gatcagccag
     4921 gcgaatccgg caaccgccag cgccagcggc agaaaccacg cggcgacgcg gtcggcgagc
     4981 cgcaccacgg gcgcgctctc ggctgcggcc tgctgcgcca gccgcacgat gcccgcgtac
     5041 gtgctctcgg ccgccggtgc catcgtgtcg atttccacag ctgcaccggc gttgacggcg
     5101 ccgctgcgca ccgcctcccc gcttcggtac tcgatgtgga cggattcgcc cgtgagcacc
     5161 gactcgtcca gaacagccca ctccgaggcg atcctggcgt cgaccggaag catttcgccg
     5221 gggccgacca ccaacacgtc gcccaccacc acctcgtcga ctggcacggt ctccagatcg
     5281 tccccggttc gtcgacgtgc aacacggggg gcacgctcga gcagcgaccg cagatccttg
     5341 gcggcccgcc gctcggcggc ggactccagc gcctggccgg tggccagcat gacgccgatc
     5401 agcgcacctg ccaggtattc gtgcacggcg agcgtgccga ccaacgacag caccgcgatg
     5461 atgtcggcac ccaagtggcc acgccgaagc gccgcgacca cccaccacac cgcgggcacg
     5521 atggccacca atgtgccagc ggcccatagg atgtccgcga tgtcacgggc tcccgcgagc
     5581 catgcgacgc cgccggcaac gagcgccgcg atcgtggtga ccagcaggac gcggataacg
     5641 gtccggcgca ccatcacgca cctacccctg cggtgccggg gactggatgc gaccggcgtg
     5701 gctgaaccgc gcggcccggc ggtggatccg atgccggtcg ccgcgggtgc gatgaggttg
     5761 cccggccaac cgcatcggtt cgacgtagaa cagcgtcttg gtgatctcga ccaacaccag
     5821 ataggcgatg gtcatcccgg cgagcgcggc gaagaattgc catggcagcg gcgtgaaccc
     5881 gagattgtgt gcccacggcg acacggtcag gagcaccccg accgcgatga ccgtcaacgt
     5941 gctgatcgtc aacagcagcc caggcctgct tttcaggaac ggcactctgc gggtgcggat
     6001 cgcgaagatg atcagcgtct gcgtggccag cgactccaag aaccacccgg tgcggaactc
     6061 gacggcgccc gcgtgcaaca cgcccaacag caagccgaac gtcatgaagt cgaacagcga
     6121 gctgatggga ccgaaagtca acatgaaccg ccggatgaac gcgatgttcc agtgcgacgg
     6181 ggcgtgcagc tgctcctcgt cgacatggtc gctgggaatg gccaattgcg aagtgtcgta
     6241 aagcaaattg ttcagcagga tctggctggg cagcatcggc agaaacggca gcaccgccga
     6301 cgccgctgcc ggactgaaca tgttgccgaa gttgctcgac gtgcccatca gcacgtactt
     6361 gatcgtgttg gcgaagatgc gccggccttc ggcgatgccc gtcgccagca caccgaggtc
     6421 cttttccagc agcaccacgt cagccgcgtc cttggcgacg tccgtggcag tgtccaccga
     6481 gatgccgaca tcggcggaat gcagcgccaa cgcatcgttg actccgtcgc ccaggaatcc
     6541 gaccgaccgg ccgctgcgcc gcgccgccga caccaggcgc cccttctgct cgggggtgat
     6601 ccgtgcgaat atcgtgttct ggcgtgccgc ccggacaaaa ccgtcgtcgt cgaatccttc
     6661 catctccaca ctggtgatgg tgcccttgga cgccaaaccg atttcagcac agaccttttc
     6721 ggcgacctga gggttgtcgc cggtggcgat cttcacctcg acgcccaggc cggcgagctg
     6781 tgtcagggag tcccgcgccg tcgccttcgg ctcgtccgcg aagaccagga agccgcgaag
     6841 cgccagtccg cactcgtcct cggatgtgat ttccgtcagc tccgacgcag tcttgccggc
     6901 gacggcgacc acccgtctgc cgtcggcgaa cagcgcggcc agcgtgttct gtgacccggc
     6961 aggcacgtcg acgcactgag ccagcacctg ctcgcgggcg cccttgacga tcagcaaccg
     7021 acggtcaccg tcatcgacga gggcggacat ggatcgccgg atgtggtcga acggccggac
     7081 cgccagtcgg cgcgtcgtgc cggcggcagc gcgttgtgat gccggcgatt cccacagcgc
     7141 cgcgtcgagc gggtcggcgc tggcgccgcc gctctcgcga tccacgtcag tggcgagcag
     7201 cccgatgcgc cgcaccgagt cgtcgtgggc cccgtcggcg tcgaccgcat cgatcaacgt
     7261 gatgcggccc tctgtcagcg ttccggtctt gtcggtcatc agcacgtcga cgtcgccgag
     7321 gtctttcgat gcagaccaga ccagccgctt caccagcacc ttgtatcggg cgagccgccg
     7381 cgacccggt
//
LOCUS       DASWBF010000559        14925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_721838,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000559 DASWBF010000000
VERSION     DASWBF010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721838"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF874_20860"
     CDS             <1..213
                     /locus_tag="VF874_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178419.1"
                     /translation="SADKWLLQYWPSTGRALEYLRVTWGWSLMLVVIFAVLYFRYLDA
                     KAENRLQFGIDPPWMTPKDERASVNA"
     gene            210..614
                     /gene="msrB"
                     /locus_tag="VF874_20865"
     CDS             210..614
                     /gene="msrB"
                     /locus_tag="VF874_20865"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX9178420.1"
                     /translation="MMRPKLELSDAQWRERLTLEEFDVLRCAGTEQPFTGEYTDTTAE
                     GVYECRACGAELFRSTEKFESHCGWPSFFDPTRSDAVILRPDDSLGTSRTEVLCAHCH
                     SHLGHVFVGEGYPTPTDQRYCINSIALRLVPS"
     gene            complement(611..1306)
                     /gene="hemQ"
                     /locus_tag="VF874_20870"
     CDS             complement(611..1306)
                     /gene="hemQ"
                     /locus_tag="VF874_20870"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX9178421.1"
                     /translation="MARLDHDELNATIRYLMFSTFAVRPGVLGDDRAAVVDETATFLK
                     QQEDNGVVVRGVYDVAGMRADADFMFWTHADNVEALQATYSDFRRATSLGRASTPVWS
                     SVALHRPAEFNKSHIPAFLAGEEPGAYVCVYPFVRSLEWYLLPDEERRQMLAEHGMAA
                     RGYKDVRANTVPAFALGDYEWILAFEAPELHRIVDLMRDLRATDARRHVRVETPFFTG
                     PRVSVEQLVDKLP"
     gene            complement(1311..2654)
                     /locus_tag="VF874_20875"
     CDS             complement(1311..2654)
                     /locus_tag="VF874_20875"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX9178422.1"
                     /translation="MTRSYSVVGGGISGLTAAYKLRRLVGDDALITVFDPADTLGGTL
                     RTVRVAGHPVDVGAEAFVARRPEVPALLAELGMADLQIDTTGVRPLIYAGAGLHPLPE
                     GTVNGIPTSAQSLAGLVDDATVARVAAEPSQPLRWARGTDPAVADLVADRFGAQVVAR
                     SVDPLLAGVYAGSAATIGLRSAAPGVASALDNGAVSLTEAVRAALPPASSGPVFRAVG
                     GGYQRLVDELLRQSRAEVVQTAVTRLDPDGDGWQLADDRCADAVVLAVPVPQLALLVG
                     AVAPEAAAAAHGIPVASSVVLALAVPTDTALPQRSGVLVASGEPLRAKAITLSSRKWG
                     RPDDVELLRLSFGRFGDDIARTATDDELVSWAIDDLSMVFGVTVEPVEAHVARWIDAM
                     PQYGPGHGALVARLREALPDTLALAGNYLDGIGVPACIASGAAAAQRLISASVAR"
     gene            complement(2651..3700)
                     /gene="hemE"
                     /locus_tag="VF874_20880"
     CDS             complement(2651..3700)
                     /gene="hemE"
                     /locus_tag="VF874_20880"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX9178423.1"
                     /translation="MITRRDLPNAPYLAAATGRKPSRIPVWFMRQAGRSLPEYRALRT
                     QHSMLAACFDTELVCEITLQPVRRHGVDAAILFSDIVVPLRAAGIGLDIVPDVGPVIE
                     HPIRTSDDVAALKPLESKQVSPVAAAVGLLVDALGEVPLIGFAGAPFTLASYLVEGGP
                     SRHHERTKAMMLGEPQTWHALMSALTDLTIAFLQVQIDAGVDAIQVFDSWAGALSLAD
                     YTEYVFPHSARVFGALASAAIPMTHFGVGTAELLGAMSAAGATVVGVDWRTSLADAAA
                     RVQPGIALQGNLDPVVLLAGWAVVEPQARRVVDDGRRAVDVGAAGHVFNLGHGVLPDT
                     DPGIITDLVALVHSL"
     gene            3775..4356
                     /locus_tag="VF874_20885"
     CDS             3775..4356
                     /locus_tag="VF874_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HEX9178424.1"
                     /translation="MSSAEPAQFQAAVAAMNAVEVRPEIELGPIRPPQRLAPFSYALG
                     AEVRHPETAIVPERSEGDAFGRLILLYDPEGAEAWDGTMRLVAYIQADLDSAEAVDPL
                     LPEVAWSWLVDALEARAGQATALGGTVTATTSVRYGDIAGPPRAHQLELRASWTATTP
                     ELGIHVQAFCEVLEHAAGLPPTGVTDLGSRSRA"
     gene            4353..5585
                     /locus_tag="VF874_20890"
     CDS             4353..5585
                     /locus_tag="VF874_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX9178425.1"
                     /translation="MSVAADDSEAAEPEPTPLLQPTDGVPGLTVTDDEIASAAELLAS
                     GHGPFAVDAERASGFRYSNRAYLIQVRRAGAGTVLLDPVSGNGDPVHALRPIAEVLTT
                     DVWILHAADQDLPCLADIGMHPPALYDTELAGRLAGFDRVNLAAMVRRMLGLGLAKGH
                     GAADWSKRPLPPAWLNYAALDVEVLLDLKDAVERVLSEQGKTGWAEQEFEYLRHVEPA
                     PQRRDRWRRTSGIHKVRDPRALAAVREMWTVRDEIARRRDIAPGRILPDSSLVEAALA
                     DPKTVADLRALPVFGGPRQRRNAETWLAALAQARENPEPPSPPDPPNGPPPVSRWHRR
                     KPEAAARLQAVRDGLATLSEEVGVPTENLVTPDLVRRLCWDWAGGDAEDIDAFLRDGG
                     ARPWQRELVVPVLAAALS"
     gene            complement(5659..5958)
                     /locus_tag="VF874_20895"
     CDS             complement(5659..5958)
                     /locus_tag="VF874_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178426.1"
                     /translation="MALVGPNSLWRNEFAARLMLKLGFYRPGRKASRLAMPLPMCVCD
                     QDATTPMPPAVKAAERASRAEVRHHPYGHFDIFHDPQVKADQVAFLQRTVESVAA"
     gene            complement(6073..7998)
                     /gene="dxs"
                     /locus_tag="VF874_20900"
     CDS             complement(6073..7998)
                     /gene="dxs"
                     /locus_tag="VF874_20900"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX9178427.1"
                     /translation="MLEQIRGPADLQRLSQQQLTDLAAEIRQFLIHKVAATGGHLGPN
                     LGVVELTLALHRVFDSPHDPIVFDTGHQSYVHKMLTGRCHDFDTLRKKDGLSGYPSRA
                     ESGHDWVESSHASSALSYADGLAKAFELTGQPNRHVVAVVGDGALTGGMCWEALNNIA
                     AGGRPIVIVVNDNGRSYAPTIGGFAEHLATLRLRPGYERMLESGRRAIRGMPVIGELC
                     FQAMHSIKVGIKDALAPQVMFTDLGLKYVGPVDGHDEHAVESALRSARGYGGPVIVHV
                     VTRKGMGYPPAENDEAEQMHSTGVIDPLTGMATKVAAPSWTSAFADTLVAHAAKRRDI
                     VAITAAMPGPTGLSKFGERFPDRLFDVGIAEQHALTSAAGLAMGGLHPVVAIYSTFLN
                     RAFDQIMMDVALHKLPVTMVLDRAGITGNDGASHNGTWDMSMLGIVPGIRVAAPRDAA
                     RLREELGEALDVNDGPTALRFPKGDVGEDISALRRIGVVDVLAEPAAGLSSDVLLVSV
                     GALGAMALAVAERLRNQGIGVTVVDPRWVLPVPDAVFELAAHHKLVVTMEDHGVAGGI
                     GSAVSAALRRAEIDVPCRDVGVPQQFLAHASRKEVLADVGLTDQNVARQITGWVAALG
                     SAESERAETESSARKFE"
     gene            complement(8027..9256)
                     /locus_tag="VF874_20905"
     CDS             complement(8027..9256)
                     /locus_tag="VF874_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX9178428.1"
                     /translation="MTELELTVGPPANGGSCVARHDGRVVFVRYALPGETVRARVTAD
                     KDAYWHAVAVEILEPSPDRIESLCPVAGPQGAGCCDLAFVDPAAARALKGQVVLNQLE
                     RIAGYTPASELRAERVGIGEATGWRTRVRMTVGFDGRVGYHRYHSNELVAAPNCGQLP
                     PGMLDGVTDQRFPPRAEVHVVVDDDGTRHVVSVGPWEGRGPRGRGFPRPNFRVVEGSR
                     ESVQRVGDRVWHVPVTGFWQSHRDAARVYSDLVTSWADLAPGMTAWDLYGGSGLFAAV
                     LADGVGEIGQVLSVDMSRSASRAAQGALSDLSQVRVVNGSVRRVLAGLRERPDVAVLD
                     PPRAGVGKEVVKLRADAGAPRVIHFGCEVASFARDVGLYREHGFAVEELRVFDAFPLT
                     HHVECVAILTAGASQRD"
     gene            complement(9253..11247)
                     /locus_tag="VF874_20910"
     CDS             complement(9253..11247)
                     /locus_tag="VF874_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9178429.1"
                     /translation="MSKLSTAARRIFLGQPFRSDKLAHTLLSKRIALPVFASDALSSV
                     AYAPEEIFLVLSVAGLTAYSMAPWIGLAVAAVMLVVIASYRQNVHAYPSGGGDYEVVT
                     TNLGKTAGLIVASALLVDYVLTVAVSMASAMSNIGSTLPFVAQHKTVFAVVAILLLAS
                     ANLRGIRESGTAFAIPTYAFMIGMYLMLAWGFFQLYVLGTPLRAESAGFELHSEHGDI
                     MGVALVFLVARAFSSGCAALTGVEAISNGVPAFRKPKSRNAATTLLMLGLIAVSLFMG
                     IIMLAKATGAKVAERPHEQLIGAPADYHQKTLIVQLAESVFRGFPVGLFLIAGVTALI
                     LVLAANTAFNGFPVLGSILAQDRYLPRQLHTRGDRLAFSNGILFLALAAIAFVVAFHA
                     EVTALIQLYIVGVFVSFTLSQIGMVRHWTWLLRTEEDGAVRRHMQRSRVINTIGFICT
                     GTVLVIVVLTKFMAGAWIAILAMSALFVLMKMIRRHYDSVTRELAELEAEQGEVVLPS
                     RNHAVVLVSNLHLPTLRALAYARATRPDSLEAITVNVDDAETRELVHKWEESDVTVPL
                     KVIASPYREITRPVIDYVKRVSRESPRTVVTVFIPEYVVGHWWEHVLHNQSALRLKGR
                     LLFEPGVMVTSVPWQLSSSERVKTLQPHHAPGDARRGFLD"
     gene            11347..12018
                     /locus_tag="VF874_20915"
     CDS             11347..12018
                     /locus_tag="VF874_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX9178430.1"
                     /translation="MRVVVMGCGRVGASLADALARIGHDVAIIDRDGTAFNRLSPEFT
                     GERVLGMGFDRDVLLRAGIEEAGAFAAVSSGDNSNIISARVARETFGVQRVVARIYDA
                     GRAKVYERLGIPTVATVPWTTDRLLNVLTRETETTKWRDPSGNVGVAEVVLREDWVGH
                     RVTDLEAATSARVAFMIRFGGGLLPEPKTVIQAGDQVYIAAIAGRIAEAMAIAALPPS
                     EDLGP"
     gene            12015..12677
                     /locus_tag="VF874_20920"
     CDS             12015..12677
                     /locus_tag="VF874_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX9178431.1"
                     /translation="MKVAIAGAGAVGRSIARELIENDHEIMLLERNPEHIDVDAIPAA
                     RWHLGDACELSLLESVHLEEYDVVVAATGDDKANVVVSLLAKTEFAVPRVVARVNDPR
                     NEWLFDDAWGVDVAVSTPRMLASLVEEAVAVGDLVRLMEFQQGQANLVEITLPDDTPW
                     GGRPVRKLELPRDAALVTILRGPRVIVPEADEPLEGGDELLFVATAEVEDELRSLLLP
                     EP"
     gene            complement(12695..13315)
                     /locus_tag="VF874_20925"
     CDS             complement(12695..13315)
                     /locus_tag="VF874_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HEX9178432.1"
                     /translation="MGGISGLIYSSLPVVVFVPVSTAFGLIPAIVSALSVATLILLWR
                     LWRRETVQPAVSGFIGVGICAAIAYVVGQSKGYFLLGIWGSLFWAAVFAGSVLVRRPI
                     VGYIWGFIKEHDSSWRRVRKAVWAYDIATLIWVVVFLSRFFVQRYLYDMDQTGWLGVA
                     RITMGWPLTVLAALTMYFPVRVAQRAVHQAERRDEVVQADPATSAE"
     gene            complement(13381..13752)
                     /locus_tag="VF874_20930"
     CDS             complement(13381..13752)
                     /locus_tag="VF874_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX9178433.1"
                     /translation="MATADGILRRLTRRLTEDPEQRDAEELTDEAANTGAQRAIDCQR
                     GQEVTMVGTLRSVEANAKGCAGGIKAELFDGTDAVTLVWLGQRRIAGIEPGRTLRVHG
                     RLGKLENGSKAIYNPNYEIQR"
     gene            13880..14578
                     /locus_tag="VF874_20935"
     CDS             13880..14578
                     /locus_tag="VF874_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9178434.1"
                     /translation="MAVDLGGVCAVLLPGTGSDDDYVYRAFSGALHDAGAAVVAAAPR
                     PGALIAGYLAELDNAAAETPIAVGGVSIGAAVAVAWALANPHRAVAVLAALPAWTGSP
                     EGAPAAVAARQSALALRRDGLVAATAQMRASSPQWLADELTRSWIGQWPTLPDAMEEA
                     ADYVAPTCPELERLTVPIGVVGAIDDPVHPVEVAVEWVTAAPHAALRTVTLDEIGADP
                     GTLGAACLAALQQA"
     gene            14640..>14925
                     /locus_tag="VF874_20940"
     CDS             14640..>14925
                     /locus_tag="VF874_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9178435.1"
                     /translation="MQGVERAERELLDAEALVGHLVPEGSMFAFLAAHRQDVFGDEEF
                     EDLFPSGLGRPSIPASVMASILVLQTLHDLSDRETADAARCDLRWKVATGM"
BASE COUNT         2447 a         5203 c         5069 g         2206 t
ORIGIN      
        1 tccgccgaca agtggctact ccagtactgg cccagcaccg gccgcgcgct ggaatacctg
       61 cgcgtcacat ggggctggtc gctcatgctg gtggtgatct tcgcggtgct ctacttccgc
      121 tacctcgacg ccaaggccga gaaccggctg cagttcggca tcgacccgcc ctggatgacg
      181 cccaaggatg agcgcgctag cgtgaacgca tgatgcgccc gaaactcgaa ctgtccgacg
      241 cacaatggcg tgagcggctc acactggagg agttcgacgt gctgcggtgc gccggcaccg
      301 agcaaccgtt caccggcgag tacaccgaca ctaccgccga gggcgtctac gaatgccggg
      361 cgtgcggggc ggagctgttc cgcagcaccg agaagttcga atcccattgc ggctggccgt
      421 ctttcttcga cccgacccgc tcggacgcgg tgatcctgcg gcccgatgac tcgctcggaa
      481 ccagccgcac agaggtgctg tgcgcgcact gccacagcca cctgggtcat gtgttcgtcg
      541 gcgagggcta ccccaccccg accgatcagc gctactgcat caattccatt gcgctgcggc
      601 tagttccgag ctagggcagc ttgtcgacga gctgctccac cgacacccgc ggcccggtga
      661 agaacggcgt ctcgacgcgc acgtgccggc gcgcgtcggt ggcgcgcaag tcgcgcatca
      721 agtcgacgat gcggtgcaac tccggcgcct cgaacgccag aatccactcg tagtccccca
      781 gcgcgaacgc cggaacggta ttggctcgca cgtccttgta gccgcgggcg gccatgccgt
      841 gctcggcaag catctgacga cgctcctcgt cgggcagcag ataccactcg agcgagcgca
      901 cgaacgggta cacgcatacg tatgcgccgg gctcctcacc ggccaagaac gccgggatgt
      961 ggctcttgtt gaactccgcg ggccggtgca acgcgacgct gctccacacc ggggtgctgg
     1021 ccctgcccag tgaggtcgcg cgccggaagt cggagtacgt cgcctgcagc gcctcgacat
     1081 tgtcggcgtg cgtccagaac atgaagtccg cgtcggcgcg catgccggcg acgtcataca
     1141 ccccgcgcac cacgacgccg ttgtcttcct gctgcttcag aaacgtggcg gtctcgtcga
     1201 cgacggccgc gcggtcgtca ccgagaacgc cggggcgcac cgcgaacgtc gaaaacatca
     1261 ggtagcggat cgtcgcgttg agttcgtcgt ggtccagccg agccatgccc ctatcgtgcc
     1321 acggaggccg agatgagccg ctgggccgcc gcggcgccgg acgcgatgca cgcgggcacc
     1381 ccgatgccgt cgaggtagtt gcctgccagc gccagcgtgt ccggtagcgc ctcacggagc
     1441 cgcgcgacca gcgcgccgtg gccgggtccg tactggggca tcgcgtcgat ccaccgcgcc
     1501 acgtgcgcct cgacgggctc gaccgtgaca ccgaacacca tcgacaggtc gtcgatcgcc
     1561 cacgacacca gctcgtcgtc ggttgccgtg cgagcgatgt cgtcaccgaa ccggccgaag
     1621 ctcagccgca gcagctccac gtcgtcgggg cgcccccact tacggcttga cagcgtaatt
     1681 gccttggcgc gcaacggttc tccgctggcc accaacacac cggacctctg cggcagcgcg
     1741 gtgtcggtgg gcacggccag agcgagcacc actgacgacg cgacgggaat gccgtgtgcg
     1801 gcggccgcgg cctccggggc gacggcaccg acgagcaagg cgagctgtgg cacgggcacg
     1861 gcgagcacca cggcgtcggc gcacctgtcg tcggcgagct gccagccgtc gccgtcgggg
     1921 tccaaccggg tcaccgcggt ctgcacaacc tccgcgcggc tctggcgcag caactcgtcg
     1981 acgagccgct gatacccccc gccgacagcc cggaagacgg gcccactgga ggcaggtggc
     2041 agcgccgcgc gcactgcctc cgtcaggctc accgcgccgt tgtccagcgc cgacgccacg
     2101 cccggtgcgg ccgagcgcag gccgatggtg gccgccgaac cggcgtacac gcctgcgagc
     2161 agcgggtcga ccgaccgtgc caccacctgc gcgccgaacc ggtcggccac cagatcggcg
     2221 accgcgggat ccgtgccgcg cgcccagcgc aacggctgcg acggctcggc ggcgacgcgg
     2281 gccacggtgg cgtcgtcgac gaggccggcc agcgactgcg ccgacgtggg gatgccgttc
     2341 accgtgcctt ccggcagcgg gtgcaggccg gcgccggcgt agatcagcgg acgcaccccg
     2401 gtggtgtcga tctgcaggtc ggccattccc agttcggcga gcagcgcagg cacctccggg
     2461 cggcgcgcca cgaacgcctc ggcgccgacg tccaccgggt ggcccgcgac tcgcaccgtg
     2521 cgcagcgtcc cgcccagcgt gtcggcagga tcgaacacgg tgatcagcgc gtcgtcaccg
     2581 acgaggcgac gcaacttgta ggctgcggtc agacccgaaa taccgccgcc cacaacggaa
     2641 taggaacgcg tcacagggaa tgcaccagcg ccaccaggtc cgtgatgatg cccgggtcgg
     2701 tgtcgggcag cacgccgtga ccgaggttga acacatgccc cgccgcgcct acgtccaccg
     2761 cccggcgccc gtcgtcgacc accctgcgcg cttgaggctc caccaccgcc cagccggcca
     2821 acagcaccac gggatcgagg ttgccctgca acgcgatgcc gggttgcacg cgggccgcgg
     2881 cgtcggcaag cgatgtgcgc cagtcgacgc ccaccaccgt cgcgcccgcc gcggacatcg
     2941 cgccgagcag ctcggcggtg ccgaccccga aatgcgtcat cggaatggct gccgatgcga
     3001 gagccccgaa aacccttgcg ctatgtggga atacgtactc ggtgtaatcg gccagtgaca
     3061 gcgcaccggc ccacgagtcg aacacctgga tcgcgtcgac gccggcgtcg atctgcacct
     3121 gcaggaacgc tatcgtcagg tctgtcagcg ctgacatcag cgcatgccag gtctgtggtt
     3181 ctccgagcat catcgccttg gtgcgttcgt ggtggcggct cggcccgcct tcgaccaggt
     3241 aggacgccag cgtgaacggc gcaccggcga agccgatcag cggaacctcg cccagcgcgt
     3301 cgaccagcag gccgacagcg gcggcgaccg gcgaaacctg tttggattca agcggtttca
     3361 gcgcagcgac gtcgtcagag gtgcggatcg ggtgctcgat gaccgggccg acgtcgggca
     3421 cgatgtccaa cccgatcccg gctgcgcgca gcggcaccac gatgtcggag aacagaatcg
     3481 cggcgtccac accgtggcgg cgcaccggct gcaacgtgat ctcgcagacc aactcggtgt
     3541 cgaagcaggc cgccagcatg ctgtgctgcg tccgcagcgc ccggtactcg ggcaacgagc
     3601 gacccgcctg gcgcatgaac cacaccggga tgcggctcgg cttgcggccg gtcgccgcgg
     3661 ccaggtaggg ggcattgggc aggtcacggc gggtaatcat cagcttccat gctgccatgg
     3721 ccaatccggc gcgcctgccg ccacgcggcc gtcgatggac tagcgtcgag ggtcatgagc
     3781 tccgccgaac cggctcagtt ccaggcggcc gtcgcggcga tgaacgccgt cgaggtgcgt
     3841 ccggagatcg agctcggccc cattcggccg ccgcagcggc tggcgccgtt cagctacgcg
     3901 ctgggtgccg aggtcagaca tccggagacc gcgatcgtgc cggaacggtc cgagggcgac
     3961 gcgttcgggc ggctgatcct gctctacgac cccgaaggcg cggaggcctg ggacggcacc
     4021 atgcggctgg tggcctacat ccaggccgac ctggattccg ccgaggccgt cgacccactg
     4081 ctgcccgaag tcgcgtggag ctggctggtg gacgcgctgg aggcgcgggc cggacaggcc
     4141 accgcgctgg gcggcaccgt caccgccacg acgtcggtgc gctacggaga catcgcgggg
     4201 ccgccgcggg cacatcaact cgagctgcgg gcatcgtgga ccgcgacgac ccccgagctc
     4261 ggaatccatg tgcaggcgtt ctgcgaggtg ctcgaacatg cagcgggcct gccgccgacc
     4321 ggagtgaccg atctggggtc acgttcgcgg gcgtgagtgt ggcagcagac gactcggagg
     4381 cggccgagcc tgaaccgacg cctctgctgc agccgacgga cggagtaccg ggcctgaccg
     4441 tcaccgatga cgaaatcgcc tctgccgctg agcttctcgc gtccggtcat ggaccgttcg
     4501 cggtcgacgc ggaacgcgcg tcgggtttcc ggtacagcaa ccgggcctac ttgatccaag
     4561 tgcgccgggc cggagccggg acggtgctgc tcgacccggt cagcggaaac ggcgaccctg
     4621 tgcacgcact gcggccgatc gccgaggtcc tcaccaccga cgtgtggatc ttgcacgccg
     4681 ccgaccagga tctgccgtgc ctggccgaca tcgggatgca cccgcccgcg ctgtacgaca
     4741 ccgaactggc cggccggctg gccgggttcg accgggtgaa cctcgctgcg atggtgcgcc
     4801 ggatgctcgg gctcggcctt gcgaagggtc acggcgccgc ggactggtcc aagcggccgc
     4861 tgccgccagc ctggctgaat tacgcggcgc tggacgtcga ggtgctgctg gacctcaaag
     4921 acgccgtcga acgggtgctg tccgagcaag gcaagacggg ctgggccgag caggagttcg
     4981 agtacctgcg ccacgtcgag ccggcgccgc agcggcggga ccggtggcgg cgcacgtcgg
     5041 gcattcacaa ggtgcgcgac ccgcgggcgc tggccgcggt ccgggagatg tggacggtgc
     5101 gcgacgagat cgcccgtcgc cgcgacatcg ctcccggccg gatcctgccg gactcgtcgc
     5161 tcgtggaggc cgcactggcc gaccccaaga cagtcgcgga cctgagggct ttgcccgtgt
     5221 tcggcggtcc gcggcagcgg cgcaacgccg aaacctggct cgcggcgctg gcccaggcgc
     5281 gggagaaccc ggagccgccg tcgccgcccg acccgccgaa cgggcctccg ccggtgtcgc
     5341 gctggcatcg ccgcaagccc gaggccgcgg cgcggctgca ggcggtacgt gacggcctcg
     5401 cgacgttgtc ggaggaggta ggcgtgccga cggagaacct ggttacgccg gatctcgtgc
     5461 ggcggctgtg ctgggactgg gccgggggcg atgccgaaga catcgacgcg tttctgcgcg
     5521 acggcggcgc gcggccgtgg cagcgcgagc tcgtcgtccc ggtgctggcg gcggcgctca
     5581 gctgactgcc gaacgtgaag ccatggtcgt gatgcgccga tgaagcacag ccctggcttc
     5641 agtctcggcg tgaacagact aggccgcgac gctttccacg gtgcgctgca ggaacgccac
     5701 ctggtcggcc ttgacctgcg ggtcgtgaaa gatgtcgaaa tggccgtacg ggtgatgacg
     5761 gacttcggcc cgagacgcgc gttcggcggc cttcacagcg ggcggcatcg gagtcgtcgc
     5821 atcctgatcg cacacgcaca tcggcagcgg catggccagc cgcgacgcct tgcggccagg
     5881 ccggtagaaa cccagcttca gcataagccg ggccgcaaac tcgttgcgcc acagggagtt
     5941 cggcccgaca agggccacaa cacgacgcgc ggggtcgacg ccgtcgagcc ggccacctga
     6001 tcaccgaaac agaagccatg gtcgtgatac accgcgaaac cacagccctg agttcagttt
     6061 cggcgcgact agctactcga actttcgcgc gctgctttca gtctcggcgc gctcggactc
     6121 ggccgacccc aacgcggcca cccagccggt aatctggcgg gccacattct ggtcggtgag
     6181 cccgacgtcg gcgagcacct ctttgcgcga cgcgtgcgcc aggaactgct gcggcacccc
     6241 gacgtcgcgg cacggcacgt cgatctccgc gcgccgcagc gccgccgata ccgccgagcc
     6301 gatgccgccg gcgacgccgt ggtcctccat cgtgacgaca agcttgtggt gggccgccaa
     6361 ctcgaacact gcgtccggca ccggcagcac ccaccgcgga tcgacaaccg tcacgccgat
     6421 gccctggttg cgcagccgtt ccgcgacggc caacgccatc gctcccaacg caccgaccga
     6481 caccagcagc acatccgagg acagccctgc ggcgggctcc gccaaaacgt caacgacacc
     6541 gatccgtcgc agcgccgaaa tgtcttcgcc cacatcgcct ttcggaaagc gaagtgcggt
     6601 cggcccgtcg ttgacatcca acgcctcgcc gagctcctcg cgcagccggg ccgcgtcgcg
     6661 cggcgcggcg acccggatcc cgggcacgat gcccagcatc gacatgtccc aggtgccgtt
     6721 gtggctggcg ccgtcgttgc cggtgatgcc ggcgcggtcg agcaccatcg tcaccggcag
     6781 cttgtgcagc gcgacgtcca tcatgatctg gtcgaaggcc cggttcagga aggtcgagta
     6841 gattgccacc accgggtgca gaccacccat cgccaaaccc gccgcggacg tcaacgcgtg
     6901 ctgctcggcg atgccgacgt cgaacagccg gtcggggaac cgctcgccga acttcgacag
     6961 cccagtggga ccgggcatcg cggcggtgat cgcgacgatg tcgcgccgct tagccgcgtg
     7021 cgccaccagc gtgtcggcga acgccgacgt ccagcttggc gcggccactt tcgtcgccat
     7081 gccggtcaac gggtcgatga cgccggtcga gtgcatctgc tcggcctcgt cgttctcggc
     7141 gggcggatag cccatgccct tacgggtgac gacgtgcacg atcaccgggc cgccgtagcc
     7201 gcgagcgctg cgcagcgcgc tttccaccgc atgctcgtcg tggccgtcga ccggaccgac
     7261 gtatttcagc cccaggtccg tgaacatcac ctgaggcgcc agcgcatcct tgatgcccac
     7321 cttgatgctg tgcatcgcct ggaagcacag ctcgccgatc acgggcatgc cgcggatggc
     7381 gcgccggccg ctctcgagca ttcgctcata ccccggccgc agccgtagcg tggccaggtg
     7441 ctcggcgaac ccgccgatgg tcggcgcgta gctgcggccg ttgtcgttga cgacgatcac
     7501 gatcgggcgt ccgccggcgg cgatgttgtt cagcgcctcc cagcacatgc cgccggtcaa
     7561 cgcgccgtcc ccgacgacgg cgacgacgtg gcggttgggc tggccggtca gttcgaaggc
     7621 cttcgcgagg ccgtcggcgt aggacagtgc cgacgacgca tgactggact cgacccagtc
     7681 gtgcccgctt tcggcgcggc tcgggtagcc cgacaggccg tccttcttgc gcaacgtgtc
     7741 gaagtcgtgg cagcgcccgg tcagcatctt gtgcacgtac gactgatgac cggtgtcgaa
     7801 gacgatcggg tcgtgcggcg agtcgaacac gcgatgcaga gccagcgtga gttcgaccac
     7861 accgagattc ggccccaaat gcccgccggt ggcagccact ttgtggatca ggaactgccg
     7921 gatttcggcg gccaggtcgg tgagctgctg ctgcgatagt cgttgcagat ctgcgggacc
     7981 gcggatctgt tccagcatcc agtcagtgta cgcagggagg ctgccttcag tcccgctgcg
     8041 acgcccccgc ggtaaggatt gcgacacatt ccacatggtg ggtgagcggg aacgcgtcga
     8101 acacccgcag ttcttccaca gcaaacccgt gctcgcggta taggcccacg tcgcgggcga
     8161 aggaggcaac ctcgcacccg aagtggatca cgcgcggcgc gccggcgtcg gcacgcagtt
     8221 tgaccacctc tttgccgacg ccggcgcgcg gcgggtcgag caccgcgacg tcgggtcgct
     8281 cccgcaaccc ggcgagcacc ctgcgaaccg atccgttgac cacccgaacc tgcgacaagt
     8341 cggacagcgc cccttgtgcc gcccgagacg ccgagcgcga catgtcgacg ctcagcacct
     8401 ggccgatctc gccgacgccg tcggccagca cggccgcgaa caggccggag ccgccgtaca
     8461 ggtcccacgc cgtcataccc ggcgccaggt ccgcccacga ggtcaccagg tcgctgtaga
     8521 cgcgcgccgc gtcccgatgc gactgccaga agccggtcac cgggacatgc cagacccggt
     8581 cgccgacccg ctgcaccgat tcgcgactgc cctccaccac ccgaaagttc ggacgtggga
     8641 agccccggcc ccgcggaccc cgcccctccc acggccccac gctgaccacg tggcgcgtcc
     8701 cgtcgtcgtc gaccaccacg tgcacctcgg cgcgcggcgg aaacctctgg tcggtgaccc
     8761 cgtcgagcat tcccggcggc agctgaccgc agttcggcgc ggccaccagc tcgttgctgt
     8821 gatagcggtg atagcccaca cggccgtcga agccgaccgt catccgcacc cgcgtccgcc
     8881 agcccgtcgc ctctccgatg ccgacccgct cggcgcgcag ctcactcgcc ggggtgtagc
     8941 ccgcgatgcg ctccagctgg ttgagcacca cctggccctt gagcgcccgg gcggcggccg
     9001 ggtcgacgaa cgcgaggtcg caacaccccg cgccctgtgg gccggcgacc gggcacagcg
     9061 actcgatgcg gtccggcgac ggctcgagga tctcgacggc caccgcgtgc cagtacgcgt
     9121 ccttgtccgc ggtgacccgc gcgcgcaccg tctcccccgg caacgcgtac cggacgaaca
     9181 ccacccggcc gtcgtggcgc gccacgcagc tgccaccgtt ggcgggcggc ccgacggtca
     9241 gttcgagttc ggtcaatcca ggaacccgcg gcgggcgtca ccgggcgcgt ggtgtggctg
     9301 caacgtcttc acgcgttccg acgaactcag ttgccacgga acagaagtca ccatcacccc
     9361 cggttcgaac agcagccgcc ccttcaggcg cagcgcactc tggttgtgca gcacatgctc
     9421 ccaccagtgt ccgacaacgt attccgggat gaacaccgtc acgacggttc gcggcgactc
     9481 gcggctgacc cgcttgacgt agtcgatcac cggacgggtg atctcgcggt acggcgatgc
     9541 gatcaccttc agcggcaccg tgacgtcgct ttcctcccac ttgtgcacca gctcacgggt
     9601 ttcggcgtcg tcgacgttca ccgtgatcgc ctcgagcgag tcgggccggg tggctcgcgc
     9661 gtaggccagc gcccgcaacg tcggcaggtg cagattggac accagcacca ccgcgtggtt
     9721 gcggctgggc agcacgacct cgccttgctc cgcctctagt tcggccagct cgcgcgtgac
     9781 ggagtcgtag tgccgccgaa tcattttcat cagcacgaac agcgccgaca tcgccaggat
     9841 cgcaatccac gcgccggcca tgaacttcgt gagtacgacg attacaagca ctgtgccggt
     9901 gcagatgaac ccgatcgtgt tgatcacccg ggagcgctgc atgtgccgcc gcaccgcgcc
     9961 gtcctcttcg gtgcgcagca gccacgtcca gtgccgcacc atgccgatct ggctcaacgt
    10021 gaacgacacg aacacgccga cgatgtacag ctggatgagc gcggtcacct cggcgtggaa
    10081 cgcgacgacg aacgcgatcg ccgccaacgc gaggaacaag atcccgttgg agaacgcgag
    10141 ccggtcacca cgggtgtgca actggcgcgg cagatagcgg tcctgcgcga gaatcgaacc
    10201 cagcaccggg aatccgttga acgccgtgtt ggcggccagc accaggatca gcgcggtgac
    10261 gccggcaatc agaaacagtc ccaccggaaa gccgcggaac accgactcgg cgagctgcac
    10321 gatcagcgtc ttctggtggt agtcggccgg cgcgccgatc agctgttcgt gcggtcgctc
    10381 ggcgaccttt gcgccggtgg ccttcgccag catgatgatt cccatgaaca agctcacggc
    10441 gatcagcccc agcatcaaca gcgtcgtcgc ggcgttgcgg gacttgggct tccggaatgc
    10501 cggcaccccg ttgctgatcg cttcgacgcc ggtcaatgcc gcacagccgg acgagaacgc
    10561 gcgcgcgaca aggaaaacca gcgcaacacc catgatgtcg ccgtgctcag agtgcagctc
    10621 gaatccggca gattccgcac gcaacggcgt tccgaggacg tacaactgga agaagcccca
    10681 ggccagcatc aggtacatgc cgatcatgaa cgcgtacgtc ggaatagcga atgccgtgcc
    10741 cgactcccgg atcccgcgaa gattcgccga cgccaacagc agaatcgcca cgaccgcgaa
    10801 cacagtcttg tgctgcgcga cgaacggcag cgttgagccg atgttggaca tcgccgacgc
    10861 catcgacact gcgacagtca gcacataatc caccagcagc gcactggcga cgatcaggcc
    10921 ggcggtcttc cccaggttgg tggtcaccac ctcgtagtcg ccgccgccgg acgggtaggc
    10981 gtgcacattc tggcggtagc tcgcgatcac gacaagcatg accgcggcca ccgccaatcc
    11041 gatccacggg gccatcgagt acgcggtgag gccggccacc gacagcacca ggaagatttc
    11101 ctccggcgcg taggccaccg acgacaacgc gtcggatgcg aacaccggca gggcgatccg
    11161 cttggacagc agggtgtgcg ccagcttgtc gctgcggaac ggctgtccca gaaagattcg
    11221 ccgggcggcg gtcgagagct tggacacgag gaccaaccct attcgcacta gggacctgga
    11281 tgtagcgttg tgcccacagt tttcccgtgc tctcccgcgg tgccccaacc gccgaaagga
    11341 ccgaagttgc gcgtcgtggt gatgggatgc ggacgcgtcg gcgcatctct ggccgacgcg
    11401 ctggcgcgga ttggccacga cgtggccatc atcgaccgcg acggcaccgc gttcaaccgg
    11461 ttgtccccgg agttcaccgg tgaacgggtg ctcggtatgg gtttcgaccg cgacgtgctg
    11521 ctgcgggccg ggatcgaaga ggccggcgcg ttcgcggcgg tgtcctccgg ggacaattcg
    11581 aacattatct cggcgcgagt ggcgcgcgag acgttcggtg tgcagcgcgt ggtggcgcgc
    11641 atctacgatg ccggacgggc gaaggtgtat gagcggctgg gcatccccac cgtggcgacc
    11701 gtgccttgga ccaccgatcg cctgctcaac gtgttgaccc gcgagacgga aaccacgaag
    11761 tggcgcgacc cgtccggcaa cgtcggtgtc gccgaggtcg tcttgcgcga ggactgggtg
    11821 ggccaccggg tcaccgatct ggaagctgcg acctcggcac gagtggcgtt catgatccgg
    11881 ttcggcggcg gtctgctgcc cgagccgaag accgtgatcc aagccggcga ccaggtgtac
    11941 atcgccgcca ttgcgggccg catcgccgag gcgatggcga ttgccgcgct gccgcccagt
    12001 gaggatctag gcccgtgaaa gtcgcaatcg ccggagccgg cgctgtcgga cgctcgatcg
    12061 cacgagagct gatcgagaac gaccacgaga tcatgctcct ggagcgtaat cccgagcaca
    12121 tcgacgttga cgcgatcccc gccgcacgct ggcatctcgg cgacgcctgc gagctgtcac
    12181 tgctcgagtc ggtgcatctg gaggagtacg acgtcgtcgt cgcggccacc ggcgacgaca
    12241 aggcgaacgt cgtggtgagc ctgctcgcga agaccgagtt cgcggtgccg cgcgtggtgg
    12301 cccgggtcaa cgacccgcgc aacgagtggc tattcgacga cgcgtggggc gtcgacgtcg
    12361 cggtgtccac gccgaggatg ctggcctcgc tggtcgagga ggcggtcgcg gttggagacc
    12421 tggtgcggct catggagttt cagcagggcc aggcgaacct cgtcgagatc acgctgcccg
    12481 acgacacgcc gtggggcggc aggccggtgc gaaagttgga gctgccgcgc gacgccgccc
    12541 tggtgacgat tcttcgcggc ccgcgggtga tcgtgccgga ggccgacgag ccgctggaag
    12601 gcggcgacga gctgctgttc gtggcgaccg cggaggtcga ggacgagctg cgcagcctgc
    12661 tgctgcccga accgtaagcg tcgggggcta ttcgctactc ggcgctagtt gccggatcgg
    12721 cctgcaccac ctcgtcgcgg cgctcggcct ggtgaaccgc gcgctgagcc acccggaccg
    12781 ggaagtacat cgtgagcgcg gcgagcaccg tcagcggcca ccccatcgtg atgcgggcca
    12841 cgccgagcca gccggtctgg tccatgtcgt acaggtagcg ctggacgaaa aaccgcgaca
    12901 ggaacaccac gacccagatc agcgtcgcaa tgtcgtacgc ccagacggct tttcgcaccc
    12961 ggcgccagct cgagtcgtgc tccttgatga agccccagat gtagccgacg atcggccgtc
    13021 gcaccagaac cgagccggcg aacacggcgg cccagaacag cgaaccccaa atcccgagca
    13081 ggaaatagcc tttgctctgc ccgaccacat aggcgatggc ggcgcagatg cccacgccga
    13141 tgaaccccga caccgccggc tgcaccgtct cgcgccgcca taaccgccac agaaggatca
    13201 gcgtcgcgac actcaacgcc gacacgatcg cggggatcaa cccgaacgcg gtcgataccg
    13261 ggacgaacac caccacaggc agcgacgagt agatcagccc gctgatgccg cccatctggt
    13321 cgagcagctc acgcgggacg ctgcgtttgt cggtcacagg tctccggcgg tcaagaaggg
    13381 tcagcgctga atctcgtaat tcgggttgta gatcgccttg gatccgttct cgagcttgcc
    13441 gagccgcccg tgcacccgca gtgtgcggcc gggctcgatg cccgcgatgc gccgctggcc
    13501 cagccagacg agggtgacgg cgtcggtgcc gtcgaaaagc tcggccttga tcccgccggc
    13561 gcatcctttc gcgttggctt cgacgctgcg cagtgtgccg accatcgtga cttcctggcc
    13621 gcgctgacaa tcgatggccc gctgcgcgcc ggtgtttgcg gcctcgtcgg tcagctcttc
    13681 ggcgtcacgt tgctcagggt cttcggtcag ccgacgggtg agccggcgca atattccatc
    13741 ggccgtagcc atgaccaact cctgacacgt ctgtctgtat gcaccctgca ttcgcaacgg
    13801 tagacctgtt gggggcgtcg tgccatgtgg cgtcgacgtt gctgggcgtg tcgttcggca
    13861 cagggggcag gatcgtgtgg tggccgtcga cctaggtgga gtctgcgcgg tgttgctgcc
    13921 cggcaccgga tccgacgacg actacgtcta ccgggcattt tccggagcgt tgcacgacgc
    13981 gggcgcggcg gtggtggcgg ccgcgccacg gccgggcgcg ttgatcgcgg gctacctcgc
    14041 agagctcgac aacgccgccg ccgaaacgcc gatcgcggtt ggcggcgtat caatcggtgc
    14101 cgcggtcgcg gtggcttggg cgctcgcgaa cccgcaccgc gcggtggcgg tgctggccgc
    14161 gttgcccgcc tggacggggt cgccggaggg cgcgcccgcg gcggtggcgg cccggcagtc
    14221 ggccctggcg ctgcggcggg acggcttggt cgcggcgacg gcccagatgc gcgcgtcgag
    14281 cccgcagtgg ctcgccgacg agctcacccg ctcctggatc ggccagtggc ccacgcttcc
    14341 cgacgcgatg gaggaggccg ccgactacgt cgcgcccacg tgccccgagc tggaacggct
    14401 cacggtcccg ataggcgtcg tcggcgcgat cgacgacccg gtccatccgg tggaagtcgc
    14461 ggtggagtgg gtgacggccg cgccccacgc agcgctgcgg acggtgacgc tcgacgagat
    14521 cggcgccgat ccggggacgc tgggggcggc gtgcctggcc gcgctacaac aggcctagga
    14581 gacgctgaat aatcggcgcg cctccccgga atatcgctct gaactgggaa aataggccca
    14641 tgcagggtgt ggagcgggcg gagcgggaac tgctggacgc agaggcattg gtcggtcatc
    14701 tggtgcctga gggcagcatg ttcgcgttcc ttgccgcaca tcgccaggat gtgttcggcg
    14761 atgaggagtt cgaggacctg ttcccgtcgg ggctgggacg accgtcgatc ccggcgtcgg
    14821 tgatggcctc gatcctggtg ctgcagaccc tgcatgacct gtccgaccgg gaaaccgcag
    14881 acgcggcccg ctgtgacctg cggtggaagg tcgctaccgg aatgg
//
LOCUS       DASWBF010000560         7099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_320531,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000560 DASWBF010000000
VERSION     DASWBF010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320531"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VF874_20945"
     CDS             <1..357
                     /locus_tag="VF874_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9178436.1"
                     /translation="FEKAGIEPSDVDVIQLQDTDAGAEIIHMAEAGFCADGDQEKLIT
                     EGATEIGGSMPVNTDGGLLANGEPIGASGLRQVHELVRQLRGEAGDRQVPGDPQVGFA
                     QVYGAPGTAAATVLTL"
     gene            437..1138
                     /locus_tag="VF874_20950"
     CDS             437..1138
                     /locus_tag="VF874_20950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9178437.1"
                     /translation="MPNTTYDCPAGPLPAYLVVPSGDGPWPGVVVVHDAFGMSADTRR
                     ITDRFASSGYLALAPALYHRGVKPLCVVGTFRALQSGSGPAVDDLIAARQYLAADDRC
                     TGKVGIAGFCMGGGFCLLLAPRGVFDAAAPNYGVWPKDIDALRRSCPTVASYGAQDRM
                     LKGAAAKLETLLTEAGVPHDVKEYPDVGHSFMNDWRTSAPLQVVNRIGSYRYSKPEAE
                     DAWRRILAFFEKHLT"
     gene            complement(1225..3132)
                     /locus_tag="VF874_20955"
     CDS             complement(1225..3132)
                     /locus_tag="VF874_20955"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX9178438.1"
                     /translation="MTVPHWRRFAEYVAQRFGGPSGEYEQLWQWSVDNPARFWRAVWE
                     YFDVRGSAPAEGDAGVLADASMPGAQWFPRVELNYVDVVLRHAHQDGPAIVGVDESGV
                     RTEIGWDALPGRIGAVAAELRRLGVTQGDTVAAYLPDVADAMVAFLATASLGAVWSSC
                     GQDYAPEGAAARLSQLRPKVLFSADGYQHNGRWIDKQAHTAELRGLLNDPALIVIGGD
                     SYRDIVAEPIALDVAAVPFDHPLWVLFSSGTTGRPKGIVHGHGGIVLEHLKTVALHAD
                     LGSDDVFFWQTALSWMMWNFRLSGLLCGSTVVCYSGHPLYPDADRLWQLVADERVTYF
                     GTSPGHLLASRKAGLTPGSSHDLSRLRTIGSTGSPLAADLFEWVGSHVGKDVTVSSIS
                     GGTDVCTAFAGGTPGVPVRAGELATRYLGVALDCWSSKREPRIDEVGELVITKPMPSM
                     PVGFWNDDDGSKYRAAYFDHDWEDGPAPGVWRHGDWVTLTERGSIVIHGRSDATLNRH
                     GIRMGSADIYEVVEAIDEISEAFVVGIDGPDGKYWMPLFVAMAPGKALDAGLTDKVRN
                     EIRSKLSPRHVPDEIIAAPGIPHTRTGKKLEVPITGILAGRSDVAVDSRAIDNPDLLD
                     WYREQGKRHSW"
     gene            complement(3129..3935)
                     /locus_tag="VF874_20960"
     CDS             complement(3129..3935)
                     /locus_tag="VF874_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX9178439.1"
                     /translation="MTLQHGPAWLFCPADRPERFAKAAAVADVVILDLEDGVAAADRE
                     TARKALVDNPLDPAKTVVRVNAADTADYDADLDTLSRTEYTTVMLPKSESAQQIRALA
                     PRDVVVLVETPLGAVRVAESVQVDNAVAVMWGSEDLFAALGGTANRNADGTLRDVARH
                     VRSSSLLAAKAFSRLALDSVYLDIKDLDGLREEVDDAVAVGFDAKVAIHPSQVPVIRA
                     GYLPEADQVEWARRVVAAARESRGVFQLDGMMVDMPVLRRAQQIVRLSER"
     gene            complement(3932..4417)
                     /locus_tag="VF874_20965"
     CDS             complement(3932..4417)
                     /locus_tag="VF874_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9178440.1"
                     /translation="MTQDETKRTVEQRGLWYEEFEIGVRYLHRPGRTITEADNVLFTT
                     LTMNTQALHLDAAWSEGQPPFHQRLVNSMFTLSTLVGLSVAQLTQGTIVGNLGFSEIA
                     FPKPLFHGDTLYAESEVTDKRLSRTREGEGIVTFSHVGRNQHGDIVATATRKTMVRTK
                     P"
     gene            complement(4438..5268)
                     /locus_tag="VF874_20970"
     CDS             complement(4438..5268)
                     /locus_tag="VF874_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9178441.1"
                     /translation="MPCNPNALADRTLVISGASRGIGLAIGLGAARQGANVVLLAKTD
                     QPHPKLPGTVHTAAADIEAAGGKAAAVVGDVRKEDDVRRAVDVAVEKFGGVDICVNNA
                     SAIATEPSEDLAVKKFDLMADVNIKGTFLLTQACLPHLRKSPNPHVLTIAPPLNMNPR
                     WLGAHPAYTLSKYGMSLLSLGWAAEFADAGIAFNCLWPQTYIATTAVANIPDGDKMLA
                     SSRSPEIMADAAVEILSRPSRDATGNCYIDEEVLVAAGGYDLSRYGGGAQPIPDLFIG
                     "
     gene            5396..6973
                     /locus_tag="VF874_20975"
     CDS             5396..6973
                     /locus_tag="VF874_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9178442.1"
                     /translation="MDDQVQTSAGIPLKPVYGPADQITEPPAPGAFPFTRGNFASGYR
                     GRLWTFRQYSGFGTAEESNRRYRYLLDQGGTGLSVALDLPTQCGYDSDDPEVSEEVGR
                     VGVAVDTLADAEILFDGIPLDKISTSFTINGTAAILLAFYVAAAERKGVPRAKLTGTI
                     QNDILKEYASRGTWIWPPEPSLRLIADTIEFCAAEVPKFNAISVAGAHFRDAGANAVQ
                     EMAFTLADGVTYCDTVVERGRMTIDEFAPQISFFFYTHGDFFEEIAKYRAGRRRWATI
                     VRERYGAKSDKASMFRVGCVSGGASLYAPQAQNNIVRVAYEAMASVLGGVQSMFTAAW
                     DEPFALPSEESATLALRTQQILAYETGITKVADPLGGSYFVEALTDATEERIIEIMAD
                     LERHGGMVRAIEDGYLQGLISDEAFHIHQDIESGARLIVGLNKFVTDEPPHDIGMYEL
                     DAEGRDLQLKRLSKVKSERDGSGAKQALAALSRAAEGNDNLMHALIDCVNAYCTVGEM
                     VSTLKAVWGEFQQPVVF"
     gene            6999..>7099
                     /locus_tag="VF874_20980"
     CDS             6999..>7099
                     /locus_tag="VF874_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX9178443.1"
                     /translation="MTARVLVAKPGLDGHDRGAKIVARTLRDAGFEV"
BASE COUNT         1189 a         2371 c         2360 g         1179 t
ORIGIN      
        1 ttcgagaagg ccggtatcga accgtccgat gtcgacgtga tccagttgca ggacaccgac
       61 gccggagccg agatcatcca catggccgaa gccggtttct gcgccgacgg cgaccaggag
      121 aaactgatca ccgagggcgc caccgagatc ggtggctcga tgccggtcaa caccgacggg
      181 ggtctcctgg ccaacggcga gccgatcggc gcgtccgggt tgcggcaggt gcacgagttg
      241 gtgcgccagt tgcgcggcga ggccggcgac cgtcaggtgc caggggatcc gcaggtcggg
      301 ttcgcgcagg tctacggagc gccgggcacc gccgccgcaa cagtgttgac gctgtgattt
      361 ctagccgaaa ctgcggagag atcgggattt tcaccgaaaa cgcgatctct tcgcagtttc
      421 ggcgacatcg tggtgtatgc cgaacaccac ctatgactgt cccgccgggc cgttgcccgc
      481 ctacctcgtt gttccctcgg gcgacggacc gtggcccgga gtcgtcgtcg tccacgacgc
      541 gttcgggatg tcggcggaca cccggcgcat caccgaccgc ttcgcgtcca gtggctacct
      601 cgcgctcgcg ccggcactgt accaccgcgg cgtaaagccg ttgtgcgtcg tcggaacttt
      661 ccgggcgctg caaagcggta gcgggcccgc ggtcgacgat ctgatcgcgg ctcggcagta
      721 tctggccgca gatgatcggt gcactggaaa ggtggggatc gccggcttct gcatgggcgg
      781 cggattctgc ctgctgctgg cgccccgcgg cgtgttcgac gcggcggctc cgaattacgg
      841 cgtctggccg aaagacattg acgcgcttcg ccgctcatgc ccgacagtag cgagctatgg
      901 cgcgcaggac cgcatgctga aaggcgcggc ggccaagctc gagacgctgc tgaccgaggc
      961 cggcgttccc cacgacgtga aggaataccc ggacgtcggc cacagcttca tgaacgactg
     1021 gaggacgtcg gcaccattgc aggtagtgaa ccgcattggc agctatcggt attcgaagcc
     1081 ggaggccgag gacgcgtggc ggcgcatcct ggcgttcttc gagaagcacc tcacctgacc
     1141 cggcggtcct ccgcgactgt gcgaatttgt acgccccgcc cggcgtgtca cgtacgaaac
     1201 cgcacagtcg gcgggggagg gtactcacca actgtggcgc ttgccctgct cgcggtacca
     1261 gtcgagaaga tccgggttgt cgatcgcgcg ggagtcgacg gcgacgtcgc tgcgtccggc
     1321 gagaatgccg gtgatcggca cctcgagttt cttgccggta cgggtatgcg ggataccggg
     1381 tgccgcgatg atttcgtcgg gcacatgccg cggcgacagc ttcgaacgaa tctcgttgcg
     1441 caccttgtcc gtaagcccgg cgtcgagagc cttgccgggc gccattgcga cgaacagcgg
     1501 catccagtac ttaccgtccg gcccgtcgat gccgacgacg aacgcctcgc tgatctcgtc
     1561 gatcgcctcg acgacttcgt agatgtccgc cgatcccatc cggatgccat gccggttcag
     1621 cgtcgcgtcg ctgcggccgt gaatgacgat cgagccgcgc tcagtgagcg tgacccagtc
     1681 gccgtgtcgc cacacccccg gtgccgggcc gtcctcccag tcgtgatcga agtacgctgc
     1741 gcggtacttg gatccgtcgt cgtcgttcca gaagccgacc ggcatcgacg gcatcggctt
     1801 ggtgatcacc aactcaccga cctcgtcgat gcggggctcg cgcttggagg accaacagtc
     1861 aagcgccaca ccgaggtagc gcgtcgccaa ctcgccggcg cgcaccggca cgccgggcgt
     1921 tccgccggcg aatgccgtgc agacgtcggt tccaccactg atcgacgaca ccgtcacgtc
     1981 cttgccgacg tgcgagccga cccactcgaa aagatcggcg gccaacgggg agccggtgct
     2041 gccgatcgtg cgtaatcggc tcagatcgtg tgaggagccc ggtgtgagcc cggccttgcg
     2101 cgacgccagc aggtggcccg ggctggtgcc gaagtaggtg acccgttcgt cggccacaag
     2161 ttgccacagc cggtcggcgt ccggatacag cgggtggccg ctgtagcaca ccaccgtcga
     2221 tccgcacagc agcccggaca gccggaagtt ccacatcatc caggacagcg cggtctgcca
     2281 gaagaacacg tcgtctgaac ccagatcggc atgcaaagca acggttttca gatgctcgag
     2341 cactatgccg ccgtgcccgt gcacgatgcc tttcggccgg ccggtggtgc cggagctgaa
     2401 cagcacccac agcgggtggt cgaacggcac ggcggccacg tctaaagcaa tcggctccgc
     2461 gacgatgtcg cgatacgagt caccgcctat cacgatcagc gccgggtcat tcagcagccc
     2521 gcgcagctca gccgtgtggg cctgcttgtc gatccagcgg ccgttgtgct ggtagccgtc
     2581 ggcactgaac aataccttcg gacgaagctg cgagagccgc gccgcggcac cttcgggtgc
     2641 gtagtcctgc ccgcacgacg accagaccgc gcccagcgat gcggtggcca ggaacgcgac
     2701 catcgcgtcg gccacgtcgg gcaaatatgc ggcgacggtg tcgccttgcg tgacgccgag
     2761 gcggcgaagc tcggcagcta ccgcgccgat ccgtcccggc agtgcatccc agccgatctc
     2821 cgtgcgaacg cccgattcgt cgacgccgac aatcgcgggg ccgtcctggt gcgcgtgccg
     2881 cagcacaaca tcgacgtagt tcagttcgac gcgcggaaac cattgcgccc caggcatcga
     2941 cgcgtcggca aggacgccgg catcaccttc ggcgggcgcg gagccgcgga cgtcgaagta
     3001 ctcccacacc gcgcgccaaa accgcgctgg gttgtcgacg gaccactgcc acagttgctc
     3061 gtactcgccg gacggccccc caaagcgctg cgcaacatat tccgcgaacc ggcgccagtg
     3121 gggcacggtc atcgctcgct cagcctgacg atctgctgcg cgcgccgcag cacgggcatg
     3181 tcgaccatca tgccgtccaa ctggaacacc ccacggcttt cgcgcgccgc cgcgacgacg
     3241 cgccgtgccc actcgacctg gtcggcctcc ggcaggtacc cggcccgaat cacggggacc
     3301 tggctgggat gaatcgcgac cttggcgtcg aaaccgaccg cgaccgcgtc gtcaacctcc
     3361 tcacgcagtc cgtcgagatc cttgatgtcc agatacaccg aatccagcgc cagccgcgaa
     3421 aacgctttgg ccgcaagcag ggacgacgac cgcacatgcc gcgcgacgtc acggagcgtg
     3481 ccgtcggcgt tccggtttgc ggtgccgccc aacgccgcga acagatcctc cgaaccccac
     3541 atgaccgcga cggcgttgtc gacttgcacg ctctcggcga ccctgaccgc cccgagtggc
     3601 gtctcgacca acacgaccac gtcccgaggc gccaacgcgc gaatctgctg cgcagactcg
     3661 gatttcggca gcatcacggt cgtgtattcg gtgcgagaca aggtgtccag gtcggcgtcg
     3721 taatcggcgg tgtcggccgc gttgacgcgc accacggtct tcgccgggtc aagtgggtta
     3781 tccaccaagg cctttcgggc ggtctcgcgg tcggcggcgg ccactccgtc ctcgagatcg
     3841 aggatgacga cgtcggccac ggcggcggcc ttcgcgaacc gttccgggcg gtctgccggg
     3901 cagaacaacc acgcgggtcc gtgctgcagc gtcatggttt cgtgcgcacc atcgtcttgc
     3961 gcgtcgcggt cgcgacgatg tcgccgtgct ggttgcggcc gacatggctg aacgtcacga
     4021 tgccctcgcc ttcgcgggtg cgcgacaacc gcttgtcggt tacctccgac tccgcgtaca
     4081 aggtgtcgcc gtggaacagc ggcttcggaa aggcgatttc gctgaacccg aggttgccga
     4141 cgatcgtgcc ctgggtcagc tgggccaccg acagcccgac gagagtggac agcgtgaaca
     4201 tcgagttcac cagccgctgg tgaaacggcg gctgcccctc agaccacgcc gcgtcgaggt
     4261 gcagcgcctg ggtgttcatc gtcaacgtgg tgaacaggac gttgtcggcc tcggtgatgg
     4321 tgcggcccgg ccggtgcagg tagcgaacgc cgatctcgaa ctcctcgtac cacaggccgc
     4381 gctgctcgac cgtgcgcttc gtctcgtctt gcgtcatagg cagattgtct gcgacggcta
     4441 gccgatgaac agatcgggga tgggttgcgc tccgccgccg tagcgcgaca ggtcataccc
     4501 gccggccgcg acgaggacct cttcgtcgat gtagcagttg ccggtggcat ctcgcgacgg
     4561 ccgcgacagg atctcgacgg ccgcgtccgc catgatctcc gggctgcgcg acgacgccag
     4621 catcttgtcg ccgtcgggga tgttcgccac cgcggtcgtg gcgatgtagg tctgcggcca
     4681 caggcagttg aatgcgatgc cggcgtcggc gaattcggcg gcccagccca gcgacagcaa
     4741 cgacatcccg tacttggaca gggtgtacgc ggggtgcgcg ccgagccacc gagggttcat
     4801 gttcaacggc ggcgcgatcg tcagcacgtg cgggttgggg gacttgcgca ggtgcggcag
     4861 gcaggcctgg gtgagcagga aggtgccctt gatgttgacg tcggccatca ggtcgaactt
     4921 cttgacggcg agatcctcgc tgggctccgt ggcgatcgcg ctggcgttgt tcacgcagat
     4981 gtcgacgccg ccgaacttct cgactgcgac gtcgaccgca cggcgcacgt cgtcctcttt
     5041 gcgcacgtct ccgaccacag cggccgcctt gccgcccgcg gcttcgatgt ccgcggccgc
     5101 cgtgtgcacg gtgccgggca gctttgggtg tggttgatcg gtcttggcca gcaacacgac
     5161 gttggctccc tgccgtgccg cgccgaggcc gatcgccagc ccgatgccgc gactcgcgcc
     5221 cgaaatgacc agggtgcggt cagcgagcgc gttcggattg cagggcatca ttctcctttg
     5281 ttggctgcgc gcttcaccgc acgggtggca tggtgatacc caacacgact tcggccgtgt
     5341 cgttatcacg caatggtatt ggcattctca ttttttgcaa gtacggtatt cactgatgga
     5401 tgaccaggtg cagacctctg cggggatacc cctcaaaccg gtgtacgggc cggccgacca
     5461 gatcacggaa cccccggccc caggcgcatt cccgttcacc cggggcaact tcgcctcggg
     5521 gtatcgcgga cgactgtgga cgttccggca gtactcgggg ttcgggaccg ctgaggagtc
     5581 gaatcgccgc taccgctatc tgctcgacca gggcgggacg ggcttgtccg tcgcgctgga
     5641 cctgccgacg cagtgcggct acgactccga cgaccccgag gtctccgagg aggttggccg
     5701 cgtcggcgtc gccgtggaca cgctcgccga cgcagagatc ctgttcgacg ggatcccgct
     5761 ggacaagatc agcaccagct tcacgatcaa cgggaccgcc gcgatcctgc tcgccttcta
     5821 cgtggccgcc gccgaacgca agggcgtccc acgcgcgaag ctcaccggga cgatccagaa
     5881 cgacatcctc aaggaatacg cgtcgcgcgg cacgtggatc tggccgccgg agccgtcgct
     5941 gcgcctgatc gccgacacca tcgagttctg cgccgccgag gtgccgaagt tcaacgcgat
     6001 ctctgtggcc ggcgcccact tccgcgacgc cggcgccaac gcggtgcaag agatggcatt
     6061 caccctcgcc gacggggtca cgtactgcga cacggtggtc gagcgtggcc ggatgacgat
     6121 cgacgagttc gctccgcaga tctcgttctt cttctacacg cacggcgact tcttcgaaga
     6181 gatcgcgaag taccgtgcgg gtcgccgccg ctgggcgacg atcgtgcggg agcggtacgg
     6241 cgcgaagtcc gacaaggcgt cgatgttccg ggtcggctgc gtgtccggag gcgcgtcgct
     6301 atatgccccg caggcgcaga acaacatcgt gcgggtggcc tacgaggcga tggcgtcggt
     6361 gctcggcggc gtccagtcga tgttcaccgc tgcctgggac gagccgttcg cgttgcccag
     6421 cgaggagtcc gcgacgctgg cgttgcgcac tcagcaaatc ctggcctacg aaacgggcat
     6481 caccaaggtc gccgatccgc tcggcggctc atacttcgtc gaggcgctca ccgacgccac
     6541 cgaggaacgc atcatcgaga tcatggccga cctggagcgc cacggcggca tggtgcgggc
     6601 aatcgaggac ggctatctgc aggggctcat ctccgacgag gcgttccaca tccatcagga
     6661 catcgagtcg ggcgcccgcc tgatcgtcgg cctcaacaag ttcgtcacag acgagccgcc
     6721 gcacgacatc gggatgtacg aactcgacgc cgagggccgc gacctgcagc tgaagcgtct
     6781 gagcaaggtg aagtccgagc gggatgggtc gggagccaag caagcgctcg cggcgctgtc
     6841 gcgcgcggca gagggcaacg acaacctgat gcacgcgctg atcgactgtg tcaacgccta
     6901 ctgcacggtc ggcgagatgg tgtccaccct caaggcagtg tggggcgagt tccagcagcc
     6961 ggtggtgttc tgatggcgcg cacgcgagga gcgagaaaat gaccgcgcgg gttctggtcg
     7021 ccaagcccgg cctcgacggg cacgaccgcg gcgccaagat cgtggcccgc acgcttcgcg
     7081 acgcgggctt cgaggtgat
//
LOCUS       DASWBF010000561        16751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_722196,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000561 DASWBF010000000
VERSION     DASWBF010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16751
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722196"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1233
                     /locus_tag="VF874_20985"
                     /pseudo
     CDS             1..1233
                     /locus_tag="VF874_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976983.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1262..1852
                     /locus_tag="VF874_20990"
     CDS             1262..1852
                     /locus_tag="VF874_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178444.1"
                     /translation="MTRLARIACAGSLAICGVLGTSGVAAADDPPPDLNAPPLLMPPQ
                     PVAPAPPASTIGLPLAQAGIEGGPAGLPDLSQTGNAVLLGQAPVPSAPNTVVGTPPSL
                     NAFTNEYLVPINDVPSAPGEGTLYGVPDGEQAAPGFLAYLGRVHQAAVDGRLRGGLLG
                     QYPQWQLGEPLPGTAPAPGIQVPPGLNPPEPPPPAG"
     gene            1892..2653
                     /locus_tag="VF874_20995"
     CDS             1892..2653
                     /locus_tag="VF874_20995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9178445.1"
                     /translation="MTVVSAPDQPHPIVAQLAALQRFHVYVDIAVVVVVLALSNLVAH
                     FTTPWASVATVPAAAVGLLLLLRTRGLGWTELGLGREHWKTGLGYAVAAVALVLTVIA
                     IGALLPWTRQLFMNDRYATVSGALIASMVIIPLQTVIPEELAFRGVLHGALNRAWGFR
                     GVAAAGSLLFGLWHVVTSLGLTSGNAGFTQLFGGGVAGQLIGVLLAVAATGAAGFIFT
                     WLRKRSGSLLAPIALHWSLNGLGALAAALVWHLAT"
     gene            complement(2657..3064)
                     /locus_tag="VF874_21000"
     CDS             complement(2657..3064)
                     /locus_tag="VF874_21000"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX9178446.1"
                     /translation="MDLAAETRWPRFTASATAQTSVRSMACFPLYTHIHAWGALLLLA
                     DQPGGLDAAAEDAGEILATHTALTLDAVHHDRHYRSALGSRDIIGQAKGVLMERFDAV
                     AAFALLTRLSEESHRPVVVVAEELLETKTSTDR"
     gene            complement(3204..4223)
                     /locus_tag="VF874_21005"
     CDS             complement(3204..4223)
                     /locus_tag="VF874_21005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX9178447.1"
                     /translation="MATNRSMTAWRVQRPGPMSTRPLERVTTDIPQPADDELLVAVRA
                     CGVCRTDLHVTEGDLPVHRPGVTPGHEVVGEVLETGAQAGSTFAVGDRVGIAWLRHTC
                     GVCRYCVRGRENLCPKSRYTGWDADGGYAEYATVPAAFAHRLPTGYADAELAPLLCAG
                     IIGYRSLLRADVPPGGRLGLYGFGGSAHITAQVALAQGIEVHVMTRGDAARDLARALG
                     AASVQGATDPPPVPLDAAILFAPVGDLVLPALEALDRGGTLAVAGIHLSDIPVVNYQR
                     HLFQERQLRSVTSNTRTDAREFLAFAEQHRIEVSHPTYPLGQADQALTDLADGRIAGA
                     AVLLP"
     gene            complement(4270..8295)
                     /locus_tag="VF874_21010"
     CDS             complement(4270..8295)
                     /locus_tag="VF874_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nitrate reductase/sulfite reductase
                     flavoprotein subunit alpha"
                     /protein_id="HEX9178448.1"
                     /translation="MAVGDVSDAVTRTACSYCGVGCGIEVHTTVDATTNTPVIARISG
                     DKLHPANFGRLCTKGATHAELMRATDTRLRTAMMRSRRNENPVAAPVDQAVSEAAERL
                     RAIIDEHGPDAVAVYVSGQMSLEAQYLATKLAKGFIRTVHMESNSRLCMASAGTGYKQ
                     SLGADGPPGSYSDFEHADVFLVTGSNMADCHPILFLRMADRLNAGAKLIVVDPRRTAT
                     AERADLFLQINPGTDLALLNGMLHLLVEYEAVDADFIAEHTEGWDAMPDFLADYPPDR
                     VAAITGVAADDIRTAARMIADAGEWMSCWTMGLNQSTHGTWSTNAICDLHLATGAICR
                     PGSGPFSLTGQPNAMGGREMGYMGPGLPGQRSVLSAADRAFVEDRWGLPPNTIRGDVG
                     VGTIEMFERMAAGDIKACWIIATNPVATVANRKTVIAGLEAAELVITQDAYRETATNR
                     YADIMLPAALWAESDAVMVNSERSMTLLRQSVPPCGDARPDWQLIAQMADALGYGHAF
                     AYQSSAQVFEEITQFANPATGYDLRGASYRRLEIGPLQWPCPPDDDSDRHPIRYLNDG
                     VSQALHVTDGGHVPRLAFATPTRRAVFLARPHVDPAEMPDDDFPLVLNTGRLQHQWHT
                     MTKTGTVDKLNRLNGAPFVEVHPDDAEALGIVDGTPTEIASRRGRAILPAVVTDRVRP
                     GSCFAPFHWNDEHGENLTVNAVTSDAVDPESLQPEFKACAVALRPTAAPPAPAMHPVG
                     VSLNLDREPPPILTEIETHYLAGFFSTLPQSLADAPGLPESAPVRQAVRARIDGMLAG
                     AYAVPSEKPTGRGPLVVWASQTGNAEEFAARLAQQLDTAGRPAQLVSMADCQLSDLAD
                     ARDVLVVTSTFGDGGPPDNGADFWNRLGAPDAPPLDGVRYAVLGIGDRAYADFCGHAR
                     SIDTRLADLGATPLLERTELEAYDEEPMAQWADAVTTLIGAPVTEPARPREKPRAEPF
                     SRTSPIAVPLSRNIALTPPTADKEVRQFAFDISAHGVTYAAGDSLGVFACNNDATVTR
                     WLAATGLGGGEVVVVDGTELPLRNALTLHYDICRVTPGLLAFVTEHSIDARRGLKRLR
                     ANRSHFDTWLVGRNAIDLIERFPVVADAERWQGALVRLTPRSYSISSSPLVDPHEVHL
                     TVSVVRYRGQDGLERGGVCSTFLADRAATPVPIFLQHSPHFRPPEEPGTPIIMIGPGT
                     GIAPFRGFLHERRALGDAGHNWLFFGDRHAAHNFYYRDELLDMFRDGFLSRLDLAFSR
                     DQEERIYVQHRMIEKGAELWEWLQDGAHLYVCGDASRMAKDVDAALTKIAKTHGRMSD
                     EQAHDYKRELVAAKRYLRDVY"
     gene            complement(8390..9310)
                     /locus_tag="VF874_21015"
     CDS             complement(8390..9310)
                     /locus_tag="VF874_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0050840 - extracellular matrix binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APA family fibronectin-binding glycoprotein"
                     /protein_id="HEX9178449.1"
                     /translation="MNKPEGKSRRTGLWTTLATTAVAGSMMLPMAVAYADPPTPTPTP
                     DTTTTAPPPPADPNAPAPAPVDPNAPPPGPVDPNAPAPDANRIDVAAGGFSYVPPPGW
                     TAGDASRISYGQAVLLKTPAPGQPAPTDTSILLGKLDLRLFAGAEQDNQKAAIRLASD
                     MGEFYMPFPGTRINQETVTLNAAGVPGSAAYYEVKFTDPNKPSGQIWAAALGAVAPRQ
                     GTQPAQNNRWFVVWLGSANNPVDKAAAKALAESIRPYTPPPAAPSAPAPGEPAAPGAA
                     PGPNAPAPNGQVGVPVPVNPEEAPGMMPPS"
     gene            complement(9375..10448)
                     /locus_tag="VF874_21020"
     CDS             complement(9375..10448)
                     /locus_tag="VF874_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9178450.1"
                     /translation="MTGVAVCALARRRDVEVDFEVAAGEVVALVGANGAGKSTVLHVI
                     AGLLRPDSGSVRSGSRVLTDTASRVFVPPHARRIGLLLQDALLFPHLSVVDNVAFGPR
                     SRRRGRAAARQAARAWLEQVDAAELADRAPGELSGGQAQRVAIARALAADPDVLLLDE
                     PLAGLDVAAAAGVRTVLRSVLARDRRAAVLVTHDLVDVLTLADRVLVMDDGRIVEAGD
                     AARVVAAPRSAFAARIAGVNLVNGTVEADGSLRAADGHRWYAAGDRLAAGARAVAVFR
                     PSAVAVFRDAPHGSPRNTIAVTVDQIDAMGDGIRVRASEQTDGAPGLAADVTAEAAAE
                     MRLVAGERVYFTVKAQEVALHHA"
     gene            complement(10445..11242)
                     /locus_tag="VF874_21025"
     CDS             complement(10445..11242)
                     /locus_tag="VF874_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9178451.1"
                     /translation="MNRPAGLPRWLYIPAVVGVVFVGLPLVAMAVKVDWPNFIGLISS
                     PSSRTALLLSLRTAAASTALCLLVGVPLALVLARNDGRLGQLVRPLILLPLVLPPVVG
                     GIALLYAFGRLGLLGRYLEGAGIHIAFSTTAVVLAQTFVSLPFLVIALEGAARSAGGR
                     YEDVAATLGARPTTVWWRVTLPLLAPGLVSGAVLALARALGEFGATLTFAGSRYGVTR
                     TLPLEIYVERESDADAAVALSMLLVVVAAVVVAAVGARRLGAGEPHR"
     gene            11355..12029
                     /locus_tag="VF874_21030"
     CDS             11355..12029
                     /locus_tag="VF874_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9178452.1"
                     /translation="MEVLVTGGDTDLGRTIAETFRDDGHRVVITGARRDELEIAAKEL
                     DVDAVTCDCTDPASLADAMPLLPHHLDTIVNVPAPSFDRGDPRAYTLAERAAAWRNNL
                     DATVLAAVLMVNAIGDHLHSGGSIVNVVPENPREGSPEAAVKAALSDWTAGQAEFFGT
                     RGITINTVASGRGAQAGYEGLSTGVAASAGGEIARLALFLTTPAARHITGQTLHASHG
                     ALAHWG"
     gene            12058..12972
                     /locus_tag="VF874_21035"
     CDS             12058..12972
                     /locus_tag="VF874_21035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9178453.1"
                     /translation="MTIRLGYQIPNFSYGTPVSELFPTVIRQAQEADESGFDAVMVMD
                     HFYQLPMLGTPEQPMLEAYTALGALSTATKNVQLGTLVTGNTYRNPTLLAKIITTLDV
                     VSAGRAVLGIGTGWFELEHDSLGFEFGTITDRFNRLEEALQIILPMIKGERSTFSGDW
                     YHTEEAVANPRLRDRIPLLIGGRGEKKTFRLAAQHFDHLNIIGGFDELPGKLAALRQR
                     CEEVGRDPATLETSMLVTALIDDNVSADQIPEEMKQRMVAGSADSVAEQIKTKALDAG
                     LDGVIINLPFYSPGIVAKAGETLKPLVS"
     gene            13087..14463
                     /locus_tag="VF874_21040"
     CDS             13087..14463
                     /locus_tag="VF874_21040"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9178454.1"
                     /translation="MSAQPPATAEPKRRHQVVVVGSGFGGLNVTKALKHADVDIKLIA
                     KTTHHLFQPLLYQVATGIISEGEIAPATRVILRRQKNVQVLLGDVTKIDLRNQKVTSE
                     LLGHTYVTPYDTLILAAGAGQSYFGNDHFAEFAPGMKTIDDALELRARILSAFEQAER
                     SSDPSRREKLLTFTVVGAGPTGVEMAGQIAELADHTLEGTFRRIDSTKSRVILLDAAP
                     AVLPPMGEKLGKKAAARLEKMGVEIQLGAMVTNVDRNGLTVKDSDGTTRRIESACKVW
                     SAGVSASPLGKQLAEQTSVELDPAGRVKVLPDLTIPGHPNVFVIGDMAYVDGVPGMAQ
                     GAIQGGKYAASCIKSELKGADPSEREPFQYFDKGSMATVSRYSAVAKIGPLEFGGIVA
                     WFSWLVLHLVYLVGFKTKITTLLSWTVTFIGRNRGQLTVTEQQAFARTRIEQLEEFAA
                     TVQSEAAS"
     gene            complement(14460..14861)
                     /locus_tag="VF874_21045"
     CDS             complement(14460..14861)
                     /locus_tag="VF874_21045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9178455.1"
                     /translation="MPIAPLPEFGKGFDNEIGLTYLEVTPDEGRARLDIHDKLLQPGG
                     IVHGGVYCAIVESLSSVCGHVWLSENGGGTVVGVNNNTDFLRAVGAGRVHAVTRPIHR
                     GRRQQLWLTTITDENDRVVARGQVRLQNLAD"
     gene            complement(14804..15658)
                     /locus_tag="VF874_21050"
     CDS             complement(14804..15658)
                     /locus_tag="VF874_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron reductase"
                     /protein_id="HEX9178456.1"
                     /translation="MTVLLEDPLIAGMAPATLLPLHESSRRLRELCPACPRVYGVAVM
                     ADVGRRRWWPLSAALTTDRLQTMFLDAARVMDNRAAASQLAATLAYAVVGRVVALVIL
                     EGRAWDPGLENLWVHFDSEGDIDWAGVVDPTLRVLPDNPVDGEGVVRLPNQAALATWT
                     AHRCHRALSPLFAQVHAVSRGALSVPAMWGMVGSAVVMAGTQIPQLAGSGDETGMRRG
                     QAVLDAFANFGLPVRGGQRLPVHPERGPAIDAQGPVSSAVTRRVDNPLCQLRHYPSSA
                     RGSTMKSG"
     gene            15790..16200
                     /locus_tag="VF874_21055"
     CDS             15790..16200
                     /locus_tag="VF874_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX9178457.1"
                     /translation="MAKLTRLGELERAVMDYLWSSPEPQTVRQVHEALSAQRDLAYTT
                     IMTVLQRLAKKNLVVQHRDDRAHRYAPTHGRDELVAGLMVDALDQAADSGSREAALVH
                     FVERVGADEAAALRRALADLEAKHQTPGGIPDGS"
     gene            16213..>16751
                     /locus_tag="VF874_21060"
     CDS             16213..>16751
                     /locus_tag="VF874_21060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9178458.1"
                     /translation="MSALAFALVALTLAGPVPEMLARAFWSMRAPRAAVVLWQSVAVA
                     AVLSAFSAGIAIASRLFVPALDGRPVTDEVRALGFPLWLAYVTVFAITLLIGARLVIA
                     VLQVAIATRRRRAHHRMLVDLLGAAGTGHLRIIDVSEPLAYCLPGVRSRVVVSKGALK
                     MLSDKEITAILRHERAHLRA"
BASE COUNT         2662 a         5666 c         5739 g         2684 t
ORIGIN      
        1 ccgcggctgc tcggccactg gggcaccacc cccggtttga atctgatttg cgcgcacctg
       61 aaccggatca ttcgggcccg cgatctcgat gtcgtctaca ttgcgggccc cggccacggc
      121 ggacccggac tggtggccaa cgcgtacctc gagggcacct acagcgaggt gtactccggt
      181 atcggcgagg acaccgacgg actgcgaaag ttgttccgac agttctcatt tccgggcgga
      241 atccccagcc acgtggccgc ggagacgccg ggatcgatcc acgagggcgg cgaactcggg
      301 tacgcgctgg tgcatgcgta cggcgcagca ttcgaccatc ccgacctggt ggtggcctgc
      361 gtgatcggcg acggtgaagc cgagaccggg ccactggccg gccagctggc actcgaacaa
      421 gttcctcaac ccggtcaccg acggcgcggt accagcagat caagtggttg acgcgcaacc
      481 gggaactgcc gcggcgcgcg tcgatcgcgt cgttgaatta ccttctgatc tcgcatgtct
      541 ggcggcagga ccacaacggc gcgtcgcatc aggatcccgg cttcatcgac cacgtgatga
      601 acaagcgtcc cgaggtggta cgggtctacc tgccgccgga cgccaactgt ctgctgtcga
      661 cggtcgacca ctgcctgagc agtcgcgact acgtgaacgt ggtggtcgcc ggcaagcagc
      721 cggcgctggc ctacctcgac atcgatgagg cgatcgcgca ctgcacccgc gggctggcca
      781 tctgggactg ggcaagcaac gcgaccggcg aacccgatgt ggtgctgcca tgatgcggct
      841 gcaaccgagt ccgagcaccc gcacgggctg tccgaccgcg aattcgacgg gctgttcacc
      901 gccgacaagc cggtaatctt cgcctatcac ggagatgcct ggctgatcct ccggctgacg
      961 tgccgccgca ccaaccacca ccactccatc catgtccgtg gcttcaagga gcgcggcacc
     1021 accacgacgc cgttcgacat ggtgatgctc aacgacctgg accggttcca cctggtgatg
     1081 gatgtcgtcg accgtgtcga cgggctgggc agccgcgccg cgatgctgca ccaaaagttg
     1141 gccgacgcgc gactggcggc ccggcgctac acccgcgact tcggcgagga ccaacccgaa
     1201 atcgtgaact ggacgtggga tccgaagttc tgagtggtgg cttcgtgttc agatagaacc
     1261 catgacccga ctcgcccgga tcgcctgcgc ggggtcgctc gcgatctgcg gtgtgctggg
     1321 cacgtccggc gtggccgccg ccgacgatcc gccgcccgac ctgaacgcgc cgccgctgct
     1381 gatgccgccg cagccggtcg ctccggcgcc gccggcgtca acgatcgggc tgccgctcgc
     1441 gcaagccggc atcgagggcg gcccggccgg cctgccggac ttgtcccaga ccggcaatgc
     1501 ggtcctgctg ggccaggcgc ccgtcccgtc ggccccgaac accgtcgtcg gcacgccacc
     1561 gagcctcaac gcgttcacca atgagtactt ggtcccgatc aacgacgtgc cctccgcacc
     1621 cggcgagggc acgctgtacg gggtcccgga cggcgagcag gccgcgccgg gcttcctggc
     1681 gtacctgggt cgtgtgcatc aggccgcggt cgatggccgg ctcaggggcg ggctgctggg
     1741 gcagtacccg caatggcagc tcggcgagcc gctgcccggc acggccccgg cgccgggcat
     1801 ccaggttccg cccggactca acccgccgga gccgccgccg cccgccggct gagcaggcat
     1861 gggcccagcc gacggtaaga tcgacggaat catgactgtc gtcagcgcac cggaccagcc
     1921 tcacccgatc gttgcgcaac tggcggcgct acagcgcttc cacgtctatg tcgacattgc
     1981 ggtcgtcgtg gtcgtgttgg cgctgtcgaa cttggtcgcc catttcacga caccgtgggc
     2041 cagcgtcgcc acggtgcccg cggccgctgt gggtcttctt ctgctgctgc ggacccgcgg
     2101 ccttggctgg acggagctgg ggctgggccg tgaacactgg aagaccggtc tgggctacgc
     2161 ggtcgccgcg gtggcgctgg tgctgaccgt gatcgcgatc ggcgcgctgc tgccgtggac
     2221 acgacagctc ttcatgaacg accgctacgc caccgtgtcc ggggcgctga tcgcgtcgat
     2281 ggtgatcatt ccgctgcaga ccgtgattcc cgaggagctg gcgtttcgcg gcgtactgca
     2341 cggtgcgctg aaccgggcgt ggggcttccg cggcgtcgct gcggccggct ccctgctgtt
     2401 cggcctttgg cacgtggtca catcactggg gctgaccagc gggaacgccg ggttcacgca
     2461 gttgttcggt ggtggcgtcg ccggtcagct gatcggcgtg ctgttggcgg tcgcggcgac
     2521 cggggccgcc ggcttcatct tcacctggct gcgcaagcgc agcggcagtc tgcttgcgcc
     2581 gatcgcgctg cactggtcgc tgaacggact cggtgcgctg gccgcggcgc tggtctggca
     2641 cctggcgact tgacgtctac cggtccgtgc tggtcttggt ttccaagagc tcctcggcca
     2701 cgacgaccac cggccggtgc gattcctccg acaaccgcgt gagcagagcg aacgccgcga
     2761 ccgcgtcgaa ccgctccatc aacacgccct tggcttggcc gatgatgtcc cgactgccca
     2821 gtgccgaccg gtagtgccgg tcgtgatgca ccgcatcgag cgtcagcgcc gtatgagtgg
     2881 ccaggatttc gccggcatcc tcggcggcgg cgtccagtcc gccaggctgg tctgcaagca
     2941 acagcagcgc gccccacgcg tgaatgtggg tgtagagcgg aaaacacgcc atcgaccgca
     3001 ctgacgtctg cgcggtggct gaggctgtga atcgtggcca gcgcgtctcg gcggccagat
     3061 ccatgacgcg caccgtctgc cggatccggg tgacgtcgat cgcagggccg tcgttgaggg
     3121 ccttctaagt cttgtcgctc tcagtcaaaa aacctgggaa acggcgggct gcgacgcgag
     3181 atgcaaatga atgacgttgc gcctcagggc aatagcacag cggcaccggc gatccggccg
     3241 tcggccaagt cggtgagcgc ctggtcggcc tgcccgagcg gatatgtcgg gtggctcacc
     3301 tcgatccggt gttgctcggc aaaggccagg aactcgcggg cgtcggtgcg ggtgttcgat
     3361 gttaccgacc gcagctggcg ttcttggaac aggtgccgct ggtagttcac caccgggatg
     3421 tcgctgaggt ggatgccggc caccgcgagc gtgccgccgc ggtccagtgc ctccagcgcc
     3481 ggcaacacca gatccccgac cggcgcgaac aagatcgccg cgtccagcgg caccggcggc
     3541 gggtccgtcg caccctgcac ggacgccgcg cccagcgcac gggctagatc ccgggcggcg
     3601 tcgccgcggg tcatcacgtg cacttcgatg ccttgcgcga gcgcgacctg cgcggtgatg
     3661 tgtgcgctgc cgccgaaccc gtacagcccg agccggccgc ccggcgggac atcggcgcgc
     3721 agcagcgacc ggtagccgat gatgccggcg cacagcagcg gcgccaactc ggcatcggcg
     3781 tatccggtcg gcaggcggtg ggcgaatgcg gctggcacag tggcatattc ggcgtagccg
     3841 ccgtcggcgt cccagccggt gtagcgcgac ttcgggcaca ggttctcgcg accgcgcacg
     3901 caatatcggc agactccgca ggtgtgccga agccaggcga tgccgacgcg gtcgccgacc
     3961 gcgaacgtcg accccgcctg cgccccggtt tccagcactt ccccgaccac ctcgtgcccc
     4021 ggcgtgactc caggtcggtg caccggcagg tcgccctcgg tgacgtgcag gtcggtgcgg
     4081 cacacgccgc acgcgcggac cgcgaccagc aactcgtcgt cggccggttg tgggatgtcg
     4141 gtggtgacgc gttccagcgg tcgggtgctc attgggcccg gccgctgcac ccgccaggcc
     4201 gtcatcgagc ggttcgttgc catgacctca tcgtggcgcg ggtcggcaga tttcgccgct
     4261 aatacacgtc taatacacgt cgcgaagata gcgcttggcg gcgacgagct ctcgtttgta
     4321 gtcgtgtgcc tgctcgtcgg acatgcggcc gtgggtcttg gcgatcttgg tgagcgcggc
     4381 gtcgacgtcc ttggccatcc ggctggcgtc gccgcacacg tagaggtgcg cgccgtcctg
     4441 cagccactcc cacagctccg cacccttctc gatcatgcgg tgctggacgt agatgcgttc
     4501 ctcctggtcg cgggagaagg ccaggtcgag gcggctcagg aagccgtcgc ggaacatgtc
     4561 gagcaactcg tcgcggtagt aaaagttgtg tgcggcgtgg cggtcgccga agaacagcca
     4621 gttgtggccc gcgtcgccga gcgccctgcg ctcgtgcagg aacccgcgga acggtgcgat
     4681 gccggtgccc gggccgatca tgatgatcgg cgttcccggc tcctcgggcg gccggaagtg
     4741 cggcgaatgc tgcaggaaga tcggcaccgg cgtcgccgcc cggtccgcca ggaacgtcga
     4801 gcacacccct ccgcgctcga ggccgtcctg cccgcgatac cgcaccaccg acaccgtcag
     4861 gtgaacctcg tgtgggtcga caagcgggct gctggaaatc gaatacgacc gcggcgtcaa
     4921 tcggaccagc gcgccctgcc accgctcggc atcggcgaca accgggaatc gctcgatcaa
     4981 atcgatcgcg tttcgtccga ccagccaggt atcgaaatga gatctgttgg cgcgcagccg
     5041 tttgagcccg cgccgggcgt ctatcgagtg ctcagtgacg aacgcgagca gccccggtgt
     5101 gacacgacag atgtcgtagt gcaacgtgag cgcgttgcgc agcggtagtt cggtgccgtc
     5161 gacgacgacg acctcgccgc cgccgaggcc ggtggccgcg agccacctcg tcaccgtggc
     5221 gtcgttgtta caggcgaaca caccgagcga gtcgcccgct gcgtacgtga cgccgtgcgc
     5281 cgagatgtcg aaagcgaact gacggacctc cttgtcggcg gtcggcgggg tgagtgcgat
     5341 attgcggctc aacggcacgg cgatcgggct ggtgcgtgag aagggttcgg cgcgtggctt
     5401 ttcccgtggt cttgctggtt cggttaccgg tgcgccgatc agtgtcgtca ccgcgtcggc
     5461 ccactgtgcc atcggctctt cgtcgtacgc ctccagttcc gtgcgttcca gcagcggagt
     5521 ggcgccgagg tcggcgaggc gcgtatcgat ggatcgcgcg tggccgcaga agtcggcgta
     5581 cgcccggtca ccgatgccga gcaccgcgta gcgcacaccg tccagtggcg gcgcgtccgg
     5641 cgcccccagc cggttccaga agtcggcacc gttgtcgggc ggaccgccgt cgccgaatgt
     5701 gcttgtcacc acgagaacat cgcgggcgtc ggccaggtcg gagagttggc agtcggccat
     5761 gctgaccaac tgtgcgggcc ggccggcggt gtcgagctgc tgtgcgaggc gcgcggcgaa
     5821 ctcctcggcg ttgccggtct gcgacgccca caccaccaac ggtcctcggc cagtgggctt
     5881 ctcggatggc acggcgtagg cgccggcgag catgccgtcg atacgtgcgc gcacggcctg
     5941 gcgcacaggt gcggattccg gcagccccgg cgcgtcggcc agcgactgcg gcagtgtgct
     6001 gaaaaatcct gcgagatagt gtgtttcgat ttccgtcagg atcggcggcg gttcccggtc
     6061 gaggttgagc gaaacgccga cggggtgcat ggcaggagca gggggtgccg ctgtgggccg
     6121 cagtgcgacc gcacacgcct tgaactccgg ttgcagcgac tccgggtcga cggcgtcact
     6181 cgtcaccgcg ttgaccgtga ggttctcgcc gtgttcgtcg ttccagtgaa acggcgcgaa
     6241 acagcttccg ggcctgacgc ggtcggtgac cactgcgggc aggatcgcgc gtccacggcg
     6301 ggacgcgatc tctgtcgggg tgccgtcgac gatcccgagc gcctcggcgt cgtcgggatg
     6361 cacttccacg aagggcgcgc cgttgagcct gttgagtttg tcgaccgtgc cggtcttggt
     6421 catcgtgtgc cactggtgct gcagccggcc ggtgttgagc accaacggga agtcgtcgtc
     6481 cggcatttcc gccggatcga catgcggccg tgccagaaag actgcccgtc gcgtcggtgt
     6541 cgcaaaggcg agccgcggaa cgtggccacc gtcggtcacg tgcagcgcct ggctgacgcc
     6601 gtcgttgaga tagcggatgg ggtggcggtc gctgtcgtcg tcgggcgggc acggccattg
     6661 cagcggaccg atttccagtc ggcgatagct ggcgccgcgc aggtcgtatc cggtggccgg
     6721 gttggcgaac tgggtgattt cctcgaagac ctgcgcactg gactggtagg cgaatgcatg
     6781 cccgtaaccc agggcgtctg ccatctgagc gatcagctgc cagtcgggcc tcgcgtcgcc
     6841 gcacggcggc accgactggc gcagcagcgt catcgaacgc tccgaattga ccatcaccgc
     6901 atcggattcg gcccacagtg cggccggcag catgatgtcg gcgtagcggt tggtggcagt
     6961 ttcgcggtag gcgtcctgcg tgatcaccag ctccgccgct tccaatcccg cgatcacagt
     7021 cttgcggttg gccactgtgg caacaggatt ggtcgcaatg atccagcagg ccttgatgtc
     7081 gccggcggcc atccgctcga acatttcgat ggtgcccacg ccaacgtcgc cgcgaatggt
     7141 gttgggcggc agaccccagc gatcctcgac gaaagcgcgg tcggcggccg aaagcacgga
     7201 ccgttggcca ggcagaccgg gacccatgta acccatctcg cgcccaccca tggcgttggg
     7261 ttggccggtc agggagaacg gaccgctgcc cggacggcag atggcaccgg tcgcgagatg
     7321 gagatcgcag atcgcgttgg tactccacgt gccgtgggtg ctctggttca aacccatagt
     7381 ccagcagctc atccactccc ccgcgtcggc gatcatccgc gcggcggtgc ggatgtcgtc
     7441 cgctgcgacc ccggtgatcg cggccacccg atcgggtggg taatcggcaa ggaagtcggg
     7501 catggcgtcc cagccctcgg tgtgctcggc gatgaaatcg gcgtctacgg cctcgtattc
     7561 gacgagcaga tgcagcatcc cgttgagcag cgccagatct gtgccggggt ttatctgcag
     7621 gaacaggtcg gcccgctcgg cggtagcggt gcgccgcgga tccacgacga tcagcttcgc
     7681 cccggcgttg agccgatcgg ccatgcgcag gaacaggatc ggatggcagt cggccatgtt
     7741 ggagccggtc accaagaaca cgtctgcgtg ctcgaagtcg ctgtaggagc cgggtggccc
     7801 gtcggcgccg agcgactgct tgtatcccgt gccggcgctc gccatgcaga gccgagagtt
     7861 cgactccatg tgtaccgtgc ggatgaagcc cttggccagc ttcgtggcga gatactgtgc
     7921 ctccagcgac atttgccccg acacgtacac cgcgaccgcg tcgggtccgt gctcgtcgat
     7981 aatcgcccgc aaccgctcgg cggcctcgga gaccgcctga tccactggtg ccgcaaccgg
     8041 attctcgttg cgacggcttc gcatcatcgc ggtgcgcagc cgagtgtcgg tggcgcgcat
     8101 cagctcggcg tgcgtcgcgc ccttcgtgca cagccggccg aagttggccg gatgcagttt
     8161 gtcaccggaa atgcgggcga tgacaggcgt gttcgtggtt gcgtcgacgg tcgtgtgcac
     8221 ttcgatgccg cagcccacac cgcagtacga gcatgcggtg cgcgtgacgg catcggagac
     8281 gtcgccgacg gccatgccgc tatcgtggcg gaactctgta ttcgaatgtt tccgctgagg
     8341 ttatcgcgac gtagctagct gctcacggcg cgcgccgtga ggcggtgagc taggacggcg
     8401 gcatcattcc tggcgcttcc tccggattaa ccggcaccgg caccccgacc tgcccgttcg
     8461 gcgccggtgc gttcggcccg ggcgcggcgc ctggtgcggc cggctcgccc ggcgccggtg
     8521 ccgatggcgc tgctggcggt ggcgtgtagg ggcggatcga ctccgccaag gccttggcag
     8581 cggccttgtc gaccgggttg ttggccgagc cgagccagac gacgaaccag cggttgttct
     8641 gcgcgggttg tgtgccctga cgcggcgcga cggcgccgag agcggcggcc cagatctgac
     8701 cgctgggctt gttcggatcg gtgaacttca cctcgtaata ggcagcggag ccaggcacac
     8761 ctgcggcgtt cagcgtgacc gtctcctggt tgatccgcgt gcccgggaaa ggcatgtaga
     8821 actcgcccat gtcggaggcc agtcggatgg cagccttctg attgtcctgc tcagcgccgg
     8881 cgaacaacct caggtccagc ttgcccagca ggatgctggt gtcggtggga gcgggctgtc
     8941 ccggggcggg ggtcttgagc aggacagcct ggccgtagct gatccgcgac gcatcgccag
     9001 cggtccagcc gggcggaggc acgtagctga agccgccggc ggccacatcg atccggttcg
     9061 cgtccggtgc tggcgcgttg gggtcgaccg gtccgggtgg tggcgcgttg gggtcgaccg
     9121 gcgcgggtgc cggtgcgttc gggtccgcgg gcggtggcgg tgctgtcgtt gtggtgtcag
     9181 gcgtgggcgt tggcgtgggc ggatcggcgt atgcgacggc catcggcagc atcatcgacc
     9241 cggcaaccgc cgtcgtcgcc aatgtggtcc acagccccgt acgtcgagat ttcccctccg
     9301 gcttattcat gacgacgaaa ttaccgtgtt accgccgtga cgcaagtgtg aggtgagaag
     9361 tacttagtca cttctcaggc gtggtgaagg gcgacttcct gcgccttgac ggtgaagtac
     9421 acccgttctc cggccaccag gcgcatctcg gcggcggcct ccgcggtcac gtcggccgcc
     9481 aagcccggcg ccccgtcggt ttgctcgctg gcgcgcaccc tgattccgtc acccatcgcg
     9541 tcgatctggt ccaccgtcac ggcgatcgtg ttgcgcgggc tgccgtgcgg tgcgtcgcgg
     9601 aacacggcga ctgccgacgg acgaaacacc gcgacggcac gcgcaccagc cgcgagccgg
     9661 tcgccagcgg cgtaccagcg gtggccgtca gcggcacgca gcgacccgtc agcctcgact
     9721 gtgccgttga ccaggttcac accggcgatg cgcgcggcga acgcgctgcg cggggccgcg
     9781 acgacccggg ccgcgtcgcc ggcctcgaca atccggccgt cgtccatgac caacactcga
     9841 tcagcgaggg tgagcacgtc gacgaggtcg tgggtgacca gcaccgcggc ccgacggtcg
     9901 cgggccaaca ccgagcgcag cacggttcga accccggcgg cggcggcgac gtcgagaccc
     9961 gcgagcggct cgtcgagcag cagcacgtcg ggatcagccg ccagcgcacg cgcaatggcg
    10021 acccgctgcg cctgcccgcc ggacaactcg cccggggcgc ggtcggccaa ctcggcggcg
    10081 tcgacctgtt cgagccacgc acgggcggcc tgccgcgcgg ccgcgcgccc gcggcgacgg
    10141 cttcgcggtc cgaacgcgac gttgtcgacg acggataggt gcggaaacag cagcgcgtcc
    10201 tgcagcagca gaccgatgcg ccgagcgtgc ggcgggacga agacgcgcga cgcggtgtcg
    10261 gtcagcacac ggctgccgga ccggacactg cctgagtctg gtcgcagcag tccggcgatc
    10321 acatgcagca cggtcgactt gccggcgccg ttcgccccga ccagcgcgac gacctcgccg
    10381 gcggccacct cgaagtccac ctccacgtcc cgtcgccgcg ccagcgcgca caccgccacg
    10441 ccggtcatcg gtgcggctct ccggcgccca ggcggcgcgc gccgaccgcg gccaccacga
    10501 ccgcggccac cacgaccaga agcatcgaca gcgccaccgc cgcgtcggcg tcgctttccc
    10561 gctcgacgta gatctccagc ggcagggtcc gggtgacgcc gtaccgcgac ccggcgaacg
    10621 tcagcgtcgc cccgaactcg ccgagtgccc gggccaacgc cagcacggca cccgacacga
    10681 gtccgggcgc cagcagcggc agcgtcaccc gccaccacac cgtcgtcggc cgggcaccga
    10741 gcgtcgcggc cacgtcctcg taccggccgc ccgcacttcg cgccgccccc tcgagcgcga
    10801 tcaccaggaa cggcagcgac acgaacgtct gggccagcac caccgcggtg gtggagaacg
    10861 cgatgtggat gccggccccc tccagatagc ggccgagcag cccgagccgg ccgaacgcgt
    10921 agagcagcgc gatgccgccc acgaccggcg gcagcacgag cggcagcagg atcagcggac
    10981 gcaccagctg cccgagccgg ccgtcgttgc gggccagcac cagcgccaac gggacgccca
    11041 caagcaggca caacgcggtg ctggcagcgg cggtgcgcag gctcagcagc agcgcggtgc
    11101 gggacgacgg actgctgatc agcccgatga agttcggcca gtcgaccttg acggccatcg
    11161 ccaccagcgg caagccgacg aacaccacgc ccacgaccgc gggaatgtag agccaccgcg
    11221 gcagcccggc cggccggttc acggatcaga ccctaatgtg tcgaaccctg cgcccggcgt
    11281 gcccgactgc aaccgatagc taccgtccag taacataatc cggaacatcc gcgtggacag
    11341 cgaggaggca tgcgatggag gtgcttgtca ccgggggcga caccgacctg ggccgcacga
    11401 tcgccgagac cttccgtgac gacgggcacc gggtggtcat caccggcgct cgccgcgacg
    11461 agctggaaat cgccgccaag gaactcgacg tcgacgccgt gacctgcgac tgcaccgatc
    11521 cggccagcct cgccgacgcc atgccgctgc tgccgcatca tttggacacg atcgtcaacg
    11581 tgccggcgcc gtcgttcgac cggggtgacc cgcgtgcgta cacgctggcc gaacgggccg
    11641 ccgcgtggcg caacaatctg gatgccaccg tgcttgcggc cgtgctgatg gtcaacgcga
    11701 tcggcgatca cctgcactcc ggcggctcga tcgtcaacgt ggtgcccgag aacccgcgcg
    11761 agggcagtcc cgaggccgcg gtcaaggccg cgctgtcgga ctggaccgcg gggcaggccg
    11821 aattcttcgg cacccgaggg atcacgatca acacggtggc gtcgggccgc ggcgcccagg
    11881 ccggctatga gggcctgtcc accggcgtgg cggccagtgc tggcggcgaa atcgcccgtc
    11941 tcgctttgtt tttgacgacg cctgccgcgc gccacatcac cggtcagacg ctgcacgcca
    12001 gccacggcgc gctcgcccac tggggctgac tcagacgacg gggatagggt cgaatccgtg
    12061 acgattcgac tcggctacca gatccccaac ttctcctacg gcacaccggt ctcggagctg
    12121 tttccgaccg tgatccgcca ggcgcaggaa gccgacgaaa gcggattcga cgccgtgatg
    12181 gtgatggatc acttctacca actgccgatg ctcggcaccc ctgagcagcc gatgctggag
    12241 gcgtatacgg ccctcggcgc actgtcgacg gcgaccaaga acgtgcaact gggcacgctc
    12301 gtgaccggca atacgtaccg caacccgacg ctgctcgcga agatcatcac gaccctggac
    12361 gtggtcagcg ccggccgcgc cgtgctgggc atcggcaccg gctggttcga actcgagcac
    12421 gacagcctgg gcttcgagtt cggcacgatc accgaccgct tcaaccggct cgaggaggcg
    12481 ttgcagatca tcctgccgat gatcaagggc gagcggtcga cgttctccgg cgactggtac
    12541 cacaccgagg aggccgtcgc gaatccgcgg ttacgcgacc gcattccgct gctgatcggt
    12601 ggccgcgggg agaagaagac cttccgattg gccgctcagc atttcgacca cctcaacatc
    12661 atcggcggtt tcgacgagct gcccggcaag ctggctgcgt tgaggcagcg ctgcgaggag
    12721 gtcggccgcg atccggcgac gctggagacc agcatgctgg tcaccgcgct gatcgacgac
    12781 aacgtgtcgg ccgaccagat cccggaggag atgaagcagc ggatggtggc gggcagcgcc
    12841 gactcggtcg ccgagcagat caagaccaag gcgctggacg ccggtctgga cggggtgatc
    12901 atcaacctgc cgttctactc tcccggcatc gtcgcgaagg ccggcgagac gctcaagccg
    12961 ctggtcagct gacggcagca aatacagccg ggggtggtga atcccactaa tgcatagctg
    13021 cgactgggag cagctgcccg actaccgtgg aggtagtcca gcagtttcgc ccgtcgagga
    13081 gccaccatga gcgcgcagcc cccggccacg gccgaaccga aacgtcgaca ccaggtcgtc
    13141 gtcgtcggtt cgggattcgg cgggctcaac gtgaccaagg ccctcaagca tgccgacgtc
    13201 gacatcaagc tgatcgccaa gaccacccac cacctgttcc agcccctgct gtaccaggtc
    13261 gccaccggca tcatctctga gggcgagatc gcgcccgcga cacgggtgat cctgcgccgg
    13321 caaaagaacg tccaggtgct gctcggcgac gtcaccaaga tcgatcttcg aaaccagaaa
    13381 gtgacgtcgg agctgctggg ccacacctac gtcaccccgt atgacacctt gatcctggcg
    13441 gcaggtgccg ggcagtccta cttcggcaac gaccacttcg ccgagttcgc gccgggaatg
    13501 aagaccattg acgacgcgct cgagttgcgc gcccgcatcc tctcggcgtt cgagcaggcc
    13561 gagcggtcca gcgatccgtc tcgtcgggag aagctgttga cgttcaccgt cgtcggcgcc
    13621 gggccgaccg gggtcgagat ggctggacaa atcgcggagc tcgccgacca cacgctcgag
    13681 ggcacgttcc gccgcatcga ctcgacgaag tcgcgggtga tcttgctgga cgccgcgccg
    13741 gcggtgctgc cgccgatggg cgagaagctg ggcaagaagg cggccgcccg gttggagaag
    13801 atgggtgtcg agattcagct cggcgcgatg gtcaccaatg tcgaccgcaa cgggctgacg
    13861 gtgaaggact cggacggaac aacccgccgg atcgagtcgg cttgcaaggt gtggtcggcg
    13921 ggcgtgtctg ccagcccgct gggcaagcaa ctcgctgagc agacatccgt cgagctcgac
    13981 cctgccggcc gggtgaaggt gttgcccgac ttgacaattc ccggtcaccc gaacgttttc
    14041 gtgatcggcg acatggccta cgtcgacggc gtcccgggga tggcacaggg cgccattcaa
    14101 ggcggcaagt acgccgcgtc atgcatcaag tcggagctca aaggcgccga tccgagcgag
    14161 cgtgagccat tccagtactt cgacaagggc tcgatggcca cggtgtcgcg gtatagcgcc
    14221 gtcgcgaaga tcggtccgct ggagttcggt ggcatcgtcg cgtggttttc ctggctggtg
    14281 ctgcacctgg tgtatctcgt cggcttcaaa accaagatca ccacgctgct ctcctggacc
    14341 gtcacgttca tcggccgtaa ccgcggccag ttgaccgtta ccgagcagca ggcattcgcc
    14401 cgaacacgga tcgaacagct ggaggagttt gccgcgacgg tgcaaagcga ggcggccagc
    14461 tagtcggcga ggttctgtag ccgcacctga ccccgcgcca ccacccggtc gttctcgtcg
    14521 gtgatcgtgg tcagccacag ctgctgccgg cggccgcggt gaataggcct tgtcaccgcg
    14581 tggaccctcc ctgcgccgac ggctcgcagg aagtcggtgt tgttgttcac cccgacgacg
    14641 gtgccgccac cgttttcgga gagccagacg tggccgcaga cactggacag gctctccacg
    14701 atcgcgcagt acacgccgcc atggacgatg ccccccggct gcagaagctt gtcgtggatg
    14761 tcgaggcggg ctcgtccctc gtccggggtg acctccaggt acgtcagccc gatttcattg
    14821 tcgaacccct tgccgaactc gggtagtggc gcaattggca caaaggattg tctacacgcc
    14881 gggtgacagc cgacgagacg gggccctgcg catcgatcgc ggggccccgt tcgggatgga
    14941 ccgggagtcg ttgcccgccc cgcaccggca ggccgaagtt cgcgaatgcg tcgagcaccg
    15001 cctgcccgcg ccgcatcccg gtttcatcgc ccgacccggc caattgcgga atctgtgtgc
    15061 ccgccatgac caccgcagag ccgaccattc cccacatcgc cgggaccgac agcgcgccgc
    15121 ggctgacggc gtgcacctga gcgaacagcg gcgacagcgc ccggtggcag cgatgagccg
    15181 tccaggtggc cagcgcggcc tgattcggca gccggacaac gccctcgccg tccacggggt
    15241 tgtccggcag cacacgcagg gtggggtcga cgacgcccgc ccagtcgatg tcgccttctg
    15301 agtcgaagtg cacccacagg ttctccaagc cggggtccca cgcccggccc tccaggatca
    15361 ccagcgcgac cacccgtcct actaccgcgt aggcgagcgt cgcggccagt tgcgacgcgg
    15421 cggcgcggtt gtccatgacg cgggcggcgt ccaagaacat cgtctgcagc cggtctgtgg
    15481 tcagtgccgc cgacagcggc caccaccgcc gccggccgac atcggccatc accgcgacgc
    15541 cgtatacccg cgggcaagca gggcacagtt cgcggagccg acggctggat tcgtgcagtg
    15601 gcaacagcgt tgcgggcgcc attccggcga tcagcgggtc ttcgagcagc accgtcataa
    15661 gtctccgtaa attagggtag cctaaccata acggatagag cctcggcgtg ccagactgga
    15721 tcggttacat acgacgcgca gtagtaattc gggagtacgc ttaggtgact gtcgcagggg
    15781 atgaatctga tggctaagtt gactcgattg ggtgagctcg aacgcgcggt catggattac
    15841 ttgtggtcgt cgccggagcc ccaaaccgtc cgccaggtac acgaggcgct gtccgcgcaa
    15901 cgcgacctgg cctacaccac gatcatgacg gtgttgcagc ggctcgcgaa gaagaacctc
    15961 gtggtgcagc accgggacga ccgcgcccat cgctacgcgc cgacacacgg tcgcgacgag
    16021 ctcgtggcgg ggctgatggt cgatgcgctt gatcaggctg ccgactcggg cagccgcgag
    16081 gccgcgctgg tgcacttcgt ggaacgtgtc ggcgccgacg aagccgcggc cttgaggcga
    16141 gcgctggccg acttggaagc gaaacaccag acacccggtg gcatcccgga cggaagctga
    16201 gagacttatc gcgtgtccgc gctggccttc gcactcgtag cgctgacctt ggccggccct
    16261 gtgccggaga tgctggcccg ggccttctgg tcgatgcgtg cgccccgggc cgcggttgtg
    16321 ctgtggcagt cggtcgcggt ggctgccgtg ctgtcggcgt tcagcgcggg catcgcgatc
    16381 gctagccggt tgttcgtgcc cgccctcgac ggccggcccg tcacggatga ggtcagggct
    16441 ctcggctttc cgttgtggct cgcctacgtc accgtcttcg cgatcacgct gctgatcggt
    16501 gcgcggctgg tcatcgccgt gctgcaggtg gcgatcgcga ctcggcgccg gcgcgcccac
    16561 caccgcatgc tcgtcgacct cctcggcgcc gcaggaaccg ggcatctgcg catcatcgac
    16621 gtctccgagc cgctggccta ctgtctgccc ggtgtgcgca gccgcgtcgt ggtgagcaag
    16681 ggcgcgctga aaatgttgag cgacaaagag atcaccgcga tcctgcgcca cgagcgggcc
    16741 catcttcggg c
//
LOCUS       DASWBF010000562         5797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_645555,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000562 DASWBF010000000
VERSION     DASWBF010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645555"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VF874_21065"
     CDS             complement(<1..223)
                     /locus_tag="VF874_21065"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9178459.1"
                     /translation="MFVGLDAASSESGDAWEGFLTGLGERGLRCPLLVISDGAAGLIG
                     ALERTMGAALRQRCLVHRARNVLAKVPKNA"
     gene            496..738
                     /locus_tag="VF874_21070"
     CDS             496..738
                     /locus_tag="VF874_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX9178460.1"
                     /translation="MRTTLSLDDDVLLAVKERARRENRTAGEVLSDLARQALTQQQNA
                     DPRTGPESFYGFEPFEHRGTAVSNALIDRLRDEEAV"
     gene            735..1166
                     /locus_tag="VF874_21075"
     CDS             735..1166
                     /locus_tag="VF874_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HEX9178461.1"
                     /translation="MTRALLDVNVLIALLDSDHVDHQRVRRWVDAEIVHGWASCAITQ
                     NGFVRIISQPRYPSPVPPAQAIGRLARAAATEHHEYWLCSVSLLDDELIDYSRLHGHR
                     QVTDAYLLALATANGGRFATLDQSIPIDAVRNASARNLTLI"
     gene            1300..3009
                     /locus_tag="VF874_21080"
     CDS             1300..3009
                     /locus_tag="VF874_21080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9178462.1"
                     /translation="MTVTVAAGETSTADLSAYLPLLARLRNGARPQPDWSAIVDDAVH
                     VWARPGFDTFLAIPRLRFEPFDYQLRAARTVLRRMRGRGILADEVGLGKTIEAGLVLS
                     ELRMRGLADQVLVIVPAGLVDQWRDELERKFGLPTTIAQAGSTPAREIGMDRPVTIAS
                     LAAGRRDPLLGRLTDTDWDLLIFDEAHRLRNPRSASGRLARRLRARYLLMLTATPVEN
                     KLSDLYQLVSLVAPGLLGTPAQFRAKHGAASVESRPHNTEELRARTREVMVRHRRSEV
                     AVMLPPRLAETILVTPGIDEAGLYADIVRRVRAAATGSGHTRLALRGLTRLAGSSPAA
                     AAPTLAKLGWNDLAERARAIRKPDKVRVLVELLRHHVNRDEKVLVFTGFRQTLDVLVA
                     AVERAGLSSALYHGSLTRAEKESAIAEFRAETPILLSTESAGEGRNLQFCHVMVNFDL
                     PWNPMQIEQRLGRLHRVGQEHDVTLTNLVCRGSIEHQVMHVLEAKINLFELVVGELDM
                     ILGRVDDDFDFENEVFDAFVDAVDDDEFERRLDALGNALAEARRSYVRTREGVDLLVG
                     EPS"
     gene            3006..4898
                     /locus_tag="VF874_21085"
     CDS             3006..4898
                     /locus_tag="VF874_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178463.1"
                     /translation="MTSTDAGLDFWLRYVEASGGLTEFDGDTTLVMLPPALQTMLELP
                     EVLQVTADPDVAREDGATFFSAGHPALGRSADEVLAVGDVAAMAVPVDATPPPPAEHL
                     QAMARDQFTVDHGRIDATGAPTRSVRAILRVGALVDYTLSSDDHYQECAECWVDVQSQ
                     RQLPTHVSMKLASLPTVPKDGSPSLTGLTTAIGFADRVIEQCAERRRSELGDDIDNAR
                     DAELERVKAYYRDALATIERRRAQAPADRRELLDARADSVRDERARRIGETREKYQPS
                     HEVRPFRLHIYEVPVWRLPVDVRRGDRRYPLTLDWLIPLTRFTDIACPHCGADERLVA
                     TKTRLGCVSCVPRRIAQLVPEPARPPAKPAAKAKQVVTQPPTPRQHPSQVRTDPSPTP
                     AKLGKLGDKLIGKVWDAVVRNDRRLARICAPDSPAAAAIRLYGPSGAAWAVGISPTED
                     LVAVTARTTTASSGVRHASNGYVDTAARHRFPFLLRWQTPGGTGQIDEILPYDRPFEP
                     AHLSRWVLGPGRTGVHAPPQPRIPLGPVGELLWRRVIPTFGLPSVLRCLAALWRLPDE
                     DVFLDSHGPAAVAAGIERMVCQRANLTTGPFADIAAAYRVKEATVRAADADLQKCLRL
                     GPERWW"
     gene            5293..5505
                     /locus_tag="VF874_21090"
     CDS             5293..5505
                     /locus_tag="VF874_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178464.1"
                     /translation="MAVHPDLFGNIAGSTHSELLFHLALTFGLTDDPIAGIARMAAFV
                     EAAAAAAGVGEPALQMTIGVSDGVRL"
     gene            5533..5745
                     /locus_tag="VF874_21095"
     CDS             5533..5745
                     /locus_tag="VF874_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178465.1"
                     /translation="MNTLFVSEDPESVRMLYPDNERLTHFGDDARVVVSEPLTHLPGV
                     WRKVPAGTALIIDRDIEERPFQPIDG"
BASE COUNT          975 a         1938 c         1944 g          940 t
ORIGIN      
        1 gggcgttctt gggcaccttg gccagcacgt tccgggcgcg atgcaccagg cagcgttgcc
       61 gcagcgcggc acccatggtc cgctcgagcg cgccgatcaa cccggccgcg ccgtcgctga
      121 tcaccagcag cgggcagcgc aacccgcgtt cgccgagccc ggtgaggaac ccttcccacg
      181 catcgccgga ctcactggac gccgcgtcga gcccgacgaa caccggcttg ctgacaagtg
      241 gttaatgtgc cctctggtca gcttggtgtg gtgcccacga gttggtgagc gttagccttc
      301 gcgatgtaga cgaggagcag cgggtcgaag tcgataacct tcaggcgctt ggcggaggta
      361 cgtcccgtgg acccgcgacc gccgaccgct tcgcttcagc aagagcggcg accgaaacgt
      421 ggagaaggtc ctgcagcacg caggtggctc tggctcgacc gtcgtccacc gagctgatgc
      481 gctaacatca gatgcatgcg caccacgctg tcattggatg atgacgtact gctcgcggtg
      541 aaggaacgcg cgcgccgcga gaaccgcacc gcaggcgagg tcctgtccga tcttgcccgg
      601 caggcgctga cgcaacagca aaacgccgat ccgcgtacag ggccggaaag cttctatggt
      661 ttcgaaccct tcgaacatcg ggggaccgcc gtgtccaacg ctctcatcga ccggctgcgt
      721 gacgaggaag ccgtgtgacg cgtgccctgc tggacgtcaa tgtccttatc gcactgctcg
      781 acagcgatca cgtcgaccac cagcgtgttc gccggtgggt cgacgccgag atcgtccacg
      841 gctgggcatc gtgtgcaata actcaaaacg gcttcgttcg aatcatcagc cagcctcgat
      901 accccagccc agtgccacct gcccaggcga tcggcaggtt ggcacgcgcc gcagcgaccg
      961 aacatcacga gtactggcta tgttcggtca gcctgctcga cgacgagctt atcgactact
     1021 cgcggttgca cgggcatcgc caggtaaccg acgcatatct actcgcgctc gcaaccgcca
     1081 acggcggccg gttcgcgact ctcgatcagt cgattcccat tgacgctgtc cgcaacgcca
     1141 gcgcgcgtaa cctgacgctg atctgatttc gtcaggctgg gcatcccgag aatcgcatcc
     1201 gcctcattcg tcacacatcg gcgtgggtcg tcggtcgtgt cggtggtcgc cggtactgtc
     1261 cggcgcaagc aagcacgcac caggtcgtgg ggaggccgca tgaccgtcac ggtcgccgcc
     1321 ggcgagacgt cgacagcaga cctgtccgcc tacctaccgt tgctggcgcg gctgcgcaat
     1381 ggcgcacggc cgcaaccgga ttggtccgct atcgtcgatg acgccgtcca cgtctgggca
     1441 cggccggggt tcgacacctt ccttgcgata ccgcggttgc ggttcgagcc gttcgactat
     1501 cagctgcggg ccgcgcggac cgtgctgcgg cggatgcgcg gccggggcat cctggccgac
     1561 gaggtcgggc tcggcaagac gatcgaggcc gggttggtgc tgtccgagct acgcatgcgc
     1621 gggctcgccg accaagtgct ggtgatcgtt ccggcgggac ttgtcgacca atggcgcgac
     1681 gaactcgagc ggaagttcgg gttgccgacc acgatcgccc aagccgggtc gacacctgcc
     1741 cgcgagattg ggatggaccg gccggtcact atcgcgtcgc tcgccgccgg tcgccgcgat
     1801 ccgctgctgg gtaggctgac cgacaccgac tgggacctgc tgatcttcga cgaggcgcac
     1861 cggctccgca acccccgaag cgcgtcgggc aggctcgcgc gcaggctccg cgcccgttat
     1921 ctgctgatgc tgaccgcaac gccggtggag aacaagctgt cggacctgta ccagctagtc
     1981 agcctcgtcg cgcccggact gctcggcaca cccgcccagt tccgcgccaa acatggcgcg
     2041 gcgtcggtcg agtcgagacc gcacaacacc gaggagctgc gcgcacggac ccgagaggtg
     2101 atggtcagac atcgccgcag cgaggttgcc gtgatgctgc cgccgcgcct cgccgagacg
     2161 atcttggtga cgcccggcat cgacgaggcc ggcctctacg ccgacatcgt ccggcgagtc
     2221 agggcagcgg caactgggtc gggacacacc cggttggccc tgcgcggcct gacccggcta
     2281 gccggctcca gccccgcggc cgccgcaccg acgctggcga agctcggctg gaacgacctc
     2341 gcggaacggg cccgagccat ccggaaaccg gacaaggtgc gtgtgctcgt cgaactcctt
     2401 cgacaccacg tcaacaggga cgagaaggtg ttggtgttca ccgggtttcg gcagacactg
     2461 gatgtgctcg tcgcggcggt cgagcgtgcc ggcctgtcgt cggcgttgta tcacggcagc
     2521 ctcacgcggg cggagaagga gtcggcgatc gcggagttcc gcgccgaaac gccgatcctg
     2581 ttgtcgaccg agtcggcggg tgagggtcgc aacctccagt tctgccatgt gatggtgaac
     2641 ttcgatctgc cgtggaaccc gatgcagatc gagcagcggc tcggccggct gcaccgggtc
     2701 gggcaggaac acgacgtgac gctgaccaac ctggtctgcc ggggcagcat cgaacaccag
     2761 gtcatgcacg tgctcgaggc aaagatcaac ctgttcgaac tcgtcgtcgg tgaacttgac
     2821 atgatcctcg gccgggtcga cgacgacttc gatttcgaga acgaggtttt cgatgcattc
     2881 gtcgatgccg tcgacgacga cgagttcgag cgacggctcg acgctctcgg caacgcgctc
     2941 gccgaggcga gacgcagcta tgtccgaacc agggaaggtg tcgacctcct agtgggtgag
     3001 ccgtcgtgac gagcaccgat gcggggctgg acttctggct gcggtatgtc gaggcgagcg
     3061 gcggactcac cgaattcgac ggcgatacga cgctggtgat gctcccaccg gccctgcaga
     3121 cgatgctgga gctgccggag gtgctgcagg tcaccgccga tcccgatgtc gctcgcgagg
     3181 acggtgcgac cttcttctcg gccgggcatc ccgcgctggg tcgctccgcc gacgaagtgc
     3241 tggcggtggg tgatgtcgcc gcgatggccg ttccggtgga cgccacgccg ccacccccgg
     3301 ccgagcacct gcaggcgatg gcgcgcgacc agttcaccgt cgatcacgga cggatcgacg
     3361 ccacgggcgc accgacccgt tcggttcgcg cgattctgcg cgtcggggcg ctggtcgact
     3421 acacgctgtc cagcgacgac cactaccagg aatgcgccga gtgctgggtg gacgtgcagt
     3481 cgcaacgcca gctcccaacc cacgtcagta tgaagctcgc ctccttgccg acagtgccga
     3541 aagatggttc gccatcgctc accggcctga ccacagcgat cggatttgcg gaccgggtta
     3601 tcgagcagtg cgcagagcgg cgacgatccg aactcggtga tgacatcgac aacgcgcgcg
     3661 acgcggagct tgaacgcgtc aaggcctact accgcgatgc gctcgcgacg atcgagcgtc
     3721 ggcgcgcgca agcacctgcc gatcgccggg agctgctgga cgcacgcgcc gacagcgtac
     3781 gggacgagcg cgcccggcgg atcggcgaga ctcgggagaa gtaccagccg agccacgagg
     3841 tccggccctt tcggctgcac atctacgagg tgccggtatg gcggttgccg gtcgacgtcc
     3901 gccgcggcga ccgacgttac ccgctgacgc tcgattggct catcccgctg acccgtttca
     3961 ccgacatcgc ctgcccacac tgcggtgccg atgagcgact ggtcgcgacg aagacgcggc
     4021 tcgggtgcgt cagctgcgtt ccccgccgga tcgcccagct cgtgcccgag cccgcgagac
     4081 cgccggcgaa gccagccgcg aaggcgaaac aggttgtcac gcaaccgccg acaccgaggc
     4141 agcacccgtc gcaggtgcgc accgatccca gcccgacgcc ggccaagctc gggaaactcg
     4201 gcgacaagct gatcggaaag gtgtgggacg cggtggtccg caacgaccgt cggctggcgc
     4261 gtatctgtgc gccggattcg cccgcggcgg cggcgatccg gctgtacggt ccgtcgggcg
     4321 ccgcctgggc ggtcgggatc tcgccgaccg aggacctggt cgccgtcacc gcccgcacga
     4381 caacggcgtc gtcgggcgta cgccatgcgt ccaacggcta cgtggatacc gcggcgagac
     4441 atcgcttccc gttcctgctc cggtggcaga cgccgggggg cacaggacag atcgacgaga
     4501 tcctgcccta cgacaggccg ttcgagccgg cgcacctgag ccgatgggtg ctgggacccg
     4561 gccggacggg tgtgcacgcg ccgccgcaac cccggatccc gctcggccct gtcggcgaac
     4621 tgctgtggcg gcgcgtgatt cccacgtttg gcctgcccag cgtcctgcgc tgcctcgccg
     4681 cgctgtggcg gctgcccgac gaggacgtct tcctcgattc gcacggcccc gcggcggtcg
     4741 cggccggaat cgaacgaatg gtgtgccaac gagcgaactt gacaaccggc cccttcgctg
     4801 acatcgctgc cgcctaccgc gtcaaagaag ccacagtgcg cgccgccgac gccgacctgc
     4861 agaagtgcct ccgactcggc ccggagaggt ggtggtagcc cgggacacca ccaccctgtt
     4921 cgccgcgctc aacgtgctca ccggcgtggt gatcggacga tgtcgacaca gacatgtgag
     4981 cttgctgaca agtcattaat ctacccgctc agcccagcga accgacctgc ggtgagctgc
     5041 agcgggtgga gctggcgccg gtggcggggt tgttttgctt ccctcggcga tcccacccgg
     5101 ctagcgattg tgcggcggct gagctgggcg cgcccgaagg gattcggacc cccaaccttc
     5161 tgatccgtag gcacaggccg atgacacgac caggcacgat acgagttcta ttgccggtgg
     5221 aggtgtcggg aagcccgact gtcgtcatcg ttgtcgtcag aactgtcgtc agctcacggt
     5281 gcgaaacagg ggttggctgt gcaccccgac ctgttcggca acatagcggg gtctacccat
     5341 tcggagctgc tgtttcacct cgcgctcacc ttcggtctca cggatgatcc gatcgcgggc
     5401 atcgcccgga tggcagcttt cgtcgaagcg gcggccgccg cggccggcgt gggtgaaccc
     5461 gcgctgcaga tgacgatagg ggtcagcgac ggggtgcgcc tgtaggcagc ccgttacgcc
     5521 agcggaccgg aagtgaatac gctgtttgtg agcgaagatc ccgagtcggt gcgcatgctc
     5581 taccccgaca acgagcggct gacgcacttc ggtgatgatg cccgggtggt ggtttctgag
     5641 ccgctgaccc acctgcctgg ggtgtggcgg aaagtgcccg ccgggaccgc actgattatc
     5701 gacagggaca tcgaagagcg accgttccag ccgattgatg gttgatgtga cgtggggcgg
     5761 gcgggcctcg aacccgcgac caacggatta tgagtcc
//
LOCUS       DASWBF010000563         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_123045,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000563 DASWBF010000000
VERSION     DASWBF010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123045"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VF874_21100"
     CDS             complement(<1..984)
                     /locus_tag="VF874_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9178466.1"
                     /translation="MSGCATCGTQPREGDKFCHECGAPVTVASKPAEYKQVTVLFADV
                     VRSMDIAAAVGAERLREIMAELVTHSSAIVQRYSGTVDKFTGDGIMAVFGAPIALEDH
                     AFRACLTALEIQAETRRLAVEVQHHDNITLQLRIGLNSGQVIAGEIGAGPLGYTAIGE
                     QVGMAQRMESVAPPGGVMLSESAARLVGTTALLGDPEMVHIKGSEQPVSARRLLGIGG
                     QQARRRGESPLVGRTWELNTITGILDEAINGAGCVVGVVGPAGIGKSRLVRESAAIAA
                     SRGVPVFGSYCESHASDIPFHVVARLLRGVMGVEELDDATARALVRERLPDV"
     gene            complement(1005..1820)
                     /locus_tag="VF874_21105"
     CDS             complement(1005..1820)
                     /locus_tag="VF874_21105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9178467.1"
                     /translation="MNRRQFMAGLAVAIVSGVGIARCEMGAAPQVLAASKGATPSSPP
                     ARLPGLLPPPAPESRISLPGNGALWTLPGDGDLLALTLDDGVDSDVVRMYTQFAKDTG
                     LRFTYFVNGRYRSWTDNKDLLRPLVDSGQIQLGNHTWSHQDLATLPVKRVIDEIARNH
                     TFLKNTYGANARPYLRPPYGSHNARVDAIAAALGYTVMTLWSGDLGDSKIVSEDYIVK
                     AADKFFTPQTIVIGHLNHKPVTHVYGHFVDLIRDRKLRSVTLNDIFLKPPSAA"
     gene            1896..2258
                     /locus_tag="VF874_21110"
     CDS             1896..2258
                     /locus_tag="VF874_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178468.1"
                     /translation="MHGDIDLTDLDRFAAGFPYHVFDELRRSSPVWFHPPTPHTPGGE
                     GFWVLSRYADIVDAAADAATFSSETGGEREGGGTTLEDMARDWAIGVLLNMMDDRRHA
                     KFRRLLTPSVAPRTLAAI"
     gene            2490..>2779
                     /locus_tag="VF874_21115"
     CDS             2490..>2779
                     /locus_tag="VF874_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178469.1"
                     /translation="MFAYGITLLELKRTEPADDLLSVVSHGRIDGEPLSDTEQHIFFS
                     LIIAAGGKHHEKCPRNALGASRKNARETVILARHGEWAVDVVSCRGHREVRQG"
BASE COUNT          505 a          950 c          849 g          475 t
ORIGIN      
        1 aacgtcggga agtcgctcac gcacaagggc cctagcagtc gcgtcgtcga gttcctcgac
       61 acccattacc ccgcgcagca accgggccac cacgtgaaac gggatgtcgc tggcatgcga
      121 ctcgcaataa ctgccgaaca ccggcacacc gcgactcgct gcgattgcgg cagactcgcg
      181 aaccagcctg ctcttgccga tgccggccgg ccccaccaca ccgacgacgc acccggcacc
      241 attgatcgcc tcgtcgagga ttccggtgat ggtgttcagc tcccaggtgc gtcctaccag
      301 cggcgattcc ccgcggcgcc tagcctgctg gccgccgatg cccaggagtc gcctcgccga
      361 caccggttgc tcagaaccct tgatgtgcac catctccggg tcaccgagca gtgccgtggt
      421 cccaacgagt cgcgcggcgg attcgctgag catcacccca ccgggcggcg ccacggattc
      481 catccgctgg gccatcccga cttgctcgcc aatggccgta taccccaaag gcccagcacc
      541 gatttcaccc gcgatcacct gacccgagtt cagcccgata cgcagttgca gcgtgatgtt
      601 gtcatggtgt tgaacctcaa ccgccaggcg tcgcgtttcg gcttggatct ccagcgcggt
      661 aagacacgcg cgaaaagcat ggtcttccag tgcgattggt gctccgaata ccgccatgat
      721 gccgtcgccg gtgaacttgt cgacggtgcc gctataacgc tggacgattg ccgacgagtg
      781 ggtgaccaac tcggccatga tctcgcgcag tcgctccgcc cccaccgccg cggcgatgtc
      841 cattgaccgc accacatcgg cgaacaacac cgtcacctgt ttgtactccg ccggcttgct
      901 cgcaacggtc accggcgcgc cgcactcgtg gcagaacttg tcaccctcgc ggggctgggt
      961 gccacacgtc gcgcagccgc tcacatcaat atcgtggcac cacgtcaggc agcggagggc
     1021 ggcttcaaga agatgtcgtt cagcgtgacc gaccgaagtt tgcgatctct gatcaggtcg
     1081 acgaagtggc cgtagacatg agtgaccggc ttgtggttca gatgaccgat cacgatcgtc
     1141 tgcggggtga agaacttgtc agccgctttg acgatgtagt cctcgctgac gatcttggaa
     1201 tcacccagat ctcccgacca cagcgtcatc accgtgtagc caagcgccgc cgcaatcgcg
     1261 tcaacgcgcg cgttgtggct cccgtacggc ggccgcaggt acggccgcgc gttcgcgccg
     1321 tacgtgttct tcaaaaacgt gtgattgcgc gcgatttcgt cgatcacccg tttgacgggc
     1381 agcgtggcca gatcctgatg cgaccacgtg tggttgccca actggatctg cccggagtcg
     1441 accagcggac gcagcaggtc cttgttgtcc gtccatgacc ggtagcggcc gttgacgaaa
     1501 tacgtgaacc gcagcccggt gtccttcgcg aactgtgtgt acatgcggac cacgtcactg
     1561 tcgacgccat cgtcgagcgt caacgccaac aggtcaccgt ccccgggcag agtccatagc
     1621 gccccgttgc ccggcaggct gatccgcgac tccggggccg gcggcggcag cagtcccggc
     1681 agccgcgccg gcggcgacga cggcgtagct cctttggacg ccgcgagcac ctgcggtgcc
     1741 gctcccatct cacagcgtgc gatgccgacg ccggacacga tggcgacggc gagcccggcc
     1801 atgaattggc ggcgattcag ctctgacacc gccgcgcacc gtaccaccag taacaataga
     1861 aactctgttc acacgccgtg tgagaccgta agtgcgtgca cggcgacatc gacctgacgg
     1921 acctcgaccg gttcgcggcg gggtttccct atcacgtgtt cgacgaactc cgccgaagct
     1981 cgccggtctg gtttcacccg ccgacccccc acacgcctgg cggcgaaggg ttttgggtgc
     2041 tcagccgtta cgccgacatc gtcgatgccg ccgccgacgc agccacgttc tcgtcggaga
     2101 ccggcggcga aagggaaggt ggcggaacga cgctcgagga catggcgcgc gactgggcga
     2161 tcggcgtgct gctgaacatg atggacgacc ggcggcatgc gaagttccgg cggctgctga
     2221 ccccgagcgt ggcgccgaga accttggcgg ccatttgagg ccgacctgcg ccaaagagcg
     2281 gttcacattg tcgacgccgc gctcgccaag gccgactgtg acttcctcgt cgacgtggcc
     2341 gccgagctgc cgctgcaggc ggtcacacag ctggtcgggg tgcccagcaa gaccgtcacc
     2401 acctcctgaa ctgggcgaac gtcacgctcg actacgagaa ccgtgaggtc ggcgaagcca
     2461 ccgcgcgcat ccagcaggcg tcggccgaga tgttcgcgta cggcatcacg ctgctcgagc
     2521 tcaagcgcac cgaaccggcc gacgacctgc tctcggtggt ctcgcacgga cgcatcgacg
     2581 gcgagccgct ttccgacacg gaacagcata tattcttttc cctcatcatt gcggcgggcg
     2641 gcaagcatca cgaaaagtgc ccgcgaaacg ccctcggtgc atcacgtaag aatgcccgcg
     2701 aaacggtgat cctcgccagg catggagagt gggctgtcga tgtcgtctcg tgccgaggtc
     2761 accgcgaagt tcgccaagg
//
LOCUS       DASWBF010000564        16918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_645733,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000564 DASWBF010000000
VERSION     DASWBF010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16918
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645733"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF874_21120"
     CDS             complement(<1..243)
                     /locus_tag="VF874_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX9178470.1"
                     /translation="MHPVMPRLIVQPDDGMQPVHDFIAAAQNSLLIKQFTFTEESFVD
                     AVIDRSNAGVEVRVMLNPKRSAGDRANDETYAKFKNA"
     gene            406..1113
                     /gene="prrA"
                     /locus_tag="VF874_21125"
     CDS             406..1113
                     /gene="prrA"
                     /locus_tag="VF874_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PrrA"
                     /protein_id="HEX9178471.1"
                     /translation="MVGMDSGTSPRVLVVDDDADVLASLERGLRLSGFDVATATDGAE
                     ALRSATETRPDAIVLDINMPVLDGVSVVTALRAMDNDVPVCVLSARSSVDDRVAGLEA
                     GADDYLVKPFVLAELVARVRALLRRRGSTATFSSETIQVGPLEVDIPGRRARVNGVDV
                     DLTKREFDLLAVLAEHKTAVLSRAQLLELVWGYDFAADTNVVDVFIGYLRRKLEARGA
                     PRLLHTVRGVGFVLRTQ"
     gene            1113..2480
                     /locus_tag="VF874_21130"
     CDS             1113..2480
                     /locus_tag="VF874_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9178472.1"
                     /translation="MNRLSRIFRRTPSLRTRVAFATAIGAAIPVLIVGTVVWVGITND
                     RLERLDRRLDEAAGFAVPFVPRGLEQIPPSPNDQDVVITVRKGDQVRSNASVVLPELD
                     ADYADTNVDGVRYRVRTVDIRGSEPPVSLAVGATYDDTIADTNNLHRRVIFICSFAIV
                     AAGVFGWLLAAFAVRPLKRLAQQARSIDAGDEAPDLEIRGATEAVEIADAVEGMLQRI
                     FKEQDRTKAALASARDFAAVSSHELRTPLTAMRTNLEVLATLDMPDEQRKEVLHDVIR
                     TQSRIEATLSALERLAQGELSTSDDHVPVDITELLDRAAHDAIRIYPDLDVSLMPSPS
                     VIIVGLPAGLRLAVDNAIANAVKHGGATRIQLSAVSSRAGVEIAIDDNGTGVPEEERK
                     MVFARFSRGSTASHSGSGLGLALVAQQAELHGGTASLEASPLGGARLLLRLPGPQPQN
                     AESAL"
     gene            complement(2492..2854)
                     /locus_tag="VF874_21135"
     CDS             complement(2492..2854)
                     /locus_tag="VF874_21135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9178473.1"
                     /translation="MTDKPEIDFPTGPAPRQLVIEDIVVGDGAEAVPGGTVEVHYVGV
                     EYDTGEEFDSSWSRHQSIEFPLRGLIQGWQDGIPGMRVGGRRKLTIPPEQAYGPAGGG
                     HRLSGKTLIFVIDLLTTR"
     gene            2885..3133
                     /locus_tag="VF874_21140"
     CDS             2885..3133
                     /locus_tag="VF874_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEX9178474.1"
                     /translation="MAEDNETLTRINALVAEEKDLRARLQRGEISGDEEHSRLRDLEA
                     ELDQCWDLLRQRRALRETGGDPKDAHERPADQVEGYLS"
     gene            3130..4596
                     /locus_tag="VF874_21145"
     CDS             3130..4596
                     /locus_tag="VF874_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9178475.1"
                     /translation="MTEAYDAVVVGGGHNGLVAAAYLGRAGKRVLLLERLHHVGGAAV
                     SAQPFDGVDARLSRYSYLVSLLPSRIVDELGLPVRLARRRYSSYTPDPSSGGQRGLLV
                     GPYRGIADDFYRRCRLVTRALWPTVTEPLLRRREARRLVNAEAAWVSMIDEPIGHAIR
                     DAVPDDLPRGVIATDALIGTFARLDEQTLRQNVCFLYHLLGEDWDVPIGGMGAVTDAL
                     IEAALQYGVEIVTGAQVFAVNPDGDVRYCVNDDERRVHGEFVLAGVTPTVLCSLLDEQ
                     PPSDGAPGCQVKVNLMLRRLPRLRDTGVTTEQAFGGTFHVNETFSQLESAYEMASRGE
                     VPDPLPCEIYCHSLADPSILSPSLRASGAQTLTVFGLHTPHGLGSREQLTDAVLDSLN
                     SVFAEPIQAVLMRGCIETKTTYDLEESLGMTAGNIFHGALEWPFAENDEPLDTPARRW
                     GVATSHERILLCGAGARRGGGVSGIGGHNAAMAVLEST"
     gene            complement(4681..5112)
                     /locus_tag="VF874_21150"
     CDS             complement(4681..5112)
                     /locus_tag="VF874_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9178476.1"
                     /translation="MRTSARNRLADRVWRDLTRLVLDNKDTWRRAVVTEAGLPFSRIR
                     ILNRLARQPMTIKQIAEAATIDAPAATVAVNDLADRGLVLRETDATDRRSKVVSLTDR
                     GRDVVALVKAVDDPAPASLAALSQQDLNTLRAIIEKITNER"
     gene            complement(5093..6535)
                     /locus_tag="VF874_21155"
     CDS             complement(5093..6535)
                     /locus_tag="VF874_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9178477.1"
                     /translation="MNTLSPQRKAIILSSLCLSLLIVTMDATIVNVAIPSIRADFGAS
                     PSQLQWMIDIYTLVLASLLLLSGATADRFGRRRTFQIGLTVFALGSLLCSLAPGIDAL
                     IGARLVQAIGGSMMNPVAMSIITQAFTGRVERARAIGIWGGVVGISMALGPLVGGGLI
                     QWMDWRAVFWINLPICAAAIILTAIFVPESKSASMRDVDPVGQGLGMAFLFGVVFVLI
                     EGPTMGWGNPRTLAVAVLALIAFVGFLRYESRRHDPFIDLRFFRSVPFSSATMIAVCA
                     FAAYGAFLFMMSLYLQGERHFTAAHTGLIYLPIAFGALIFSPLSGRLVGRFGSRPSLL
                     AAGTLITTATLMLTQLTVATPVWQILVLFAVFGIGFAMVNAPITTAAVSGMPTDRAGA
                     ASAVASTSRQVGVSIGVALCGSVAGAALATTGADFAVAARPLWIVCAALGAVIVFLGF
                     YSTSDRGLRSAERLAPLIAEPEENHAYIRA"
     gene            complement(6681..7988)
                     /locus_tag="VF874_21160"
     CDS             complement(6681..7988)
                     /locus_tag="VF874_21160"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX9178478.1"
                     /translation="MADNSSNAEEFATLQYPGGELELEIAHATEGADGIALGNLLSKS
                     GYTTFDPGFVNTASTKSAICYIDGDAGILRYRGYPIEQLAEKSTFIEVSYLLIYGELP
                     TKEQLAKFTTQIQRHTLLHEDLKRFFDGFPRNAHPMPVLSSAVNALSAYYQDSLDPFD
                     DGQVELSTIRLLAKLPTIAAYAYKKSAGQPFLYPDNSLTLVENFLRMTFGFPAEPYEV
                     DPEVVRALDMLFILHADHEQNCSTSTVRLVGSSQANLFTSISGGINALWGPLHGGANQ
                     AVLEMLEKIRKADGDVHDFVKKVKNREDNVKLMGFGHRVYKNYDPRARIVKEQADKIL
                     GKLGGDDELLDIAKALEEVALTDDFFVERKLYPNVDFYTGVIYRAMGFPTPMFTVLFA
                     LGRLPGWIAHWREMHDERGGKIGRPRQIYTGYGERDYKAIDSR"
     gene            complement(8081..8728)
                     /locus_tag="VF874_21165"
     CDS             complement(8081..8728)
                     /locus_tag="VF874_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9178479.1"
                     /translation="MVEAVGAGIPGLRERKKQRTRSTLIDAAVQLCEQQGYDNTTVDQ
                     IAAVADVSPRTFSRYFATKDAVIMALIDDMSDALTVQLERQPTELCPMEALLAAHSAM
                     IRATVSAPPGGFTTARMFTLARIVNSSPTLRTASGEFREHSVTHTLAMRMGVAVDDPK
                     VVMAVAVWSVILLTAFGDLGPDTDWQNVDTDMLVARLNDTYKQFTDICSPPASVT"
     gene            complement(8774..10042)
                     /locus_tag="VF874_21170"
     CDS             complement(8774..10042)
                     /locus_tag="VF874_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9178480.1"
                     /translation="MSLLADTAPLRTPDFRRLWLSGVVTVIGANLTIFAVPVQIYALT
                     QSSAYVGLSGVFALVPLIVFGLWGGAWADAMDRRVLLIIASCGLALASVLLWVQAALA
                     LNNVWLVLCLLSVQQAFYAINAPTRSAAIPRMLPDDQLPAANSLNMTVTQFGAIVGPL
                     LAGVMLRWVDLSTLYLIDAITCVAPIWATIRLSSMPPTGDGTARKGLASVWDGFRYLA
                     GNTVVLMSFVVDLIAMIFGMPRALFPQIAHESFGGPVEGGTTMALLAAAISAGAVAGG
                     VFSGWLPRIRRQGLVVVAAIVVWGLAMVGFGLCSGIAGGRAGAVLWASLAFLAVGGAA
                     DMVSAAFRSTILQQAASDDVRGRLQGVFIVVVAGGPRLADAAHGAAAAAVGTTIAAAG
                     GGALVVVGVLIAAVAVPAFVRYRVSAAAIS"
     gene            complement(10046..10708)
                     /gene="pdxH"
                     /locus_tag="VF874_21175"
     CDS             complement(10046..10708)
                     /gene="pdxH"
                     /locus_tag="VF874_21175"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX9178481.1"
                     /translation="MAQPGDKHLANMRVDYGSVEKDGSPDLDVDWLADGWVMLLRKWI
                     DDADRAGVAEPNAMVVATVDAEGRPMSRSVLCKSVDDTGITFFTNYDSAKGAELAANP
                     YASATFPWFALGRQVHIRGAVSKVTAEATADYWSKRPRGSQLGAWASAQSQPIGSRAE
                     LLAQLQKVTERFADVEEVPLPPNWGGYLIAAEVVEFWQGREDRLHNRIRLQAGRVERL
                     QP"
     gene            10790..11914
                     /locus_tag="VF874_21180"
     CDS             10790..11914
                     /locus_tag="VF874_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HEX9178482.1"
                     /translation="MTAVPENFVSGLEGVVAFTTEIAEPDKDGGALRYRGVDIEELVE
                     QRVTFADVWALLVDGKFGQSLRPAEPFPLPIHTGDVRVDVQAGLAMLAPIWGYKPILD
                     IDDQTAREQLARASVMALSYVAQSARGIERPAVPQREIDQCRTVTERFMKRWKGDPDP
                     KQVEAIDAYWVSAAEHGMNASTFTARVIASTGADVAAALSGAIGAMSGPLHGGAPARV
                     IPMIEEAESTGDAQAVVKGILDHNEKLMGFGHRVYRAEDPRARVLRAAAKRLDAPRYE
                     VAAALEQAALTELRERRPDRAIETNVEFWAAIILDFAGVPPKMMPAMFTCGRTAGWCA
                     HILEQKRLGKLVRPSAVYVGPGPRSVESVDGWSEISHAAA"
     gene            complement(11925..14000)
                     /locus_tag="VF874_21185"
     CDS             complement(11925..14000)
                     /locus_tag="VF874_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014987606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9178483.1"
                     /translation="MTLAVLVAACSRSDTATDPYLWLEELDSPQVQSWVQAENAKTLA
                     VLEQDPRYAGNLAQAVELGNAPDRLPLPALLEDGVVGNFWQDAQHKRGIWRETTVIDY
                     ESPQPQWKTVLDLDALAQAEGRNWVWEGMDCDPVTRMRCLVSLSEGGEDAVAVREFDR
                     AAGQFVPDGFVLERGKQYTTWVDADTLLVSREWQPGEMTTSGYPYVVKRWQRGQPLDQ
                     AVEVARGDKADELSTTGALLDNGAGRLLNLVVRRPTFYENQVSLLGGGQPVRMALPPK
                     ADVEGMVGDQVLVSLRQDWTTGGTTFHSGSLVSLNVDEMTLDPERLRATVVYAPGPQE
                     AMQSVMTTRDRVVVTSLYDVKGRATVYSRGPDGTWSGQPVPLPDNATVSAVAADSRGD
                     VAYLSVTSMLTPTTLYHLNTADGRVDPVKSSPARFDSSRFVVEQLKATSPDGTEVPYF
                     IVHAADLKYDGTTPTILYAYGGFESSSTPGYNGLMGKLWLERGGAYVIANIRGGGEYG
                     PAWHEAALKTKRQKAFDDFTAVARDLIARKITSPRHLGIQGGSNGGLLMGVEFTQHPE
                     LWNAVDMQIPLLDMVRYEQIAAGASWVGEYGSVANPDEREFLESISPYAQLKRGVKYP
                     EPFIFTTSKDDRVGPQHARKFAARLSEFGIPYLFYEATQGGHGAGTNIDEKARTAALE
                     YTYFQRQLM"
     gene            14194..14646
                     /locus_tag="VF874_21190"
     CDS             14194..14646
                     /locus_tag="VF874_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9178484.1"
                     /translation="MAIEVTPAVLPHLTVDNAAAAIDFYVKAFDGVELGRVPGPDGKK
                     LIHAAVQVNGTTVMLADDFPEMNDGKSTTPNALGGTPVTIHLVVTDVDTQFQRAVDAG
                     ATVVMPVEEQFWGDRYGVLRDPFGHQWSMGQPVREVSPEEIANAMQQQ"
     gene            14699..15931
                     /locus_tag="VF874_21195"
     CDS             14699..15931
                     /locus_tag="VF874_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178485.1"
                     /translation="MASPDHNEPADQPANSSDQKPSRVRAVRSAVVRPFTRTGRYYAQ
                     SRRDFRDRIATELPLVRPTLGLLAQAFRDEVVLVGLQLSRPISEPQAFERITREVEAA
                     LEMYGRNGWLDKPETYFAAPRPLTNITIHDVNRRKRSYQRFVFDSGYAPYPGEPGRER
                     WLSYEATKRVYGLMLRHREPRPWVVCVHGTEMGRARVDLRLFRAWHLHNDLGLNVALP
                     VLPLHGPRKRGVPKGAVLPGEDVLDDVHAAAHAVWDIRRVVSWIRSQEPESAIGLNGM
                     SLGGYIASLVASLEDGLTCAILGVPVADLVEILARHSGLSSDDPRRETITMARPLGRM
                     VSPLSLTPRVPMNGRFIYGGVADQVVHPRDQVVRLWEHWGKPEIVWYRGGHAGFFRSR
                     PVQDFIDAALVQSGLLHS"
     gene            complement(15850..>16918)
                     /locus_tag="VF874_21200"
     CDS             complement(15850..>16918)
                     /locus_tag="VF874_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9178486.1"
                     /translation="ELVAWINAHPDYRDLAVPLDHERVVIVGNGNVALDVARILTADP
                     KRLAHSDISRHALAALRDSKVREVVAARRRPAQSAFTLPEMIGLTAASRVVLSADNHE
                     LVHADLADCTDRMSRHKLELLASLDIYDERDDAPVIRLAYRLTPSRVLGNERAAGVEF
                     TVTGTDETLEVEAGLVLSSIGYRGWPVPGLPFDDDADVVPNRGGRAVDPSTGAPVRGS
                     YVTGWIKRGPTGFIGTNKSCAQETVNALVDDYNDGRLADPAAGLHALDRLVRRRQPDV
                     VDHARWRAIDRAEIDRGRAEGRPRDKFTTIGEMLAAHNDRPARRAVVAEALIRHEWRS
                     PDCTSAASMKSCTGRERKKPA"
BASE COUNT         2867 a         5617 c         5645 g         2789 t
ORIGIN      
        1 ggcattcttg aattttgcat acgtttcgtc gttggcgcgg tccccggctg accgcttcgg
       61 gttgagcatc acgcgcacct cgaccccggc gttcgagcgg tcgataacgg cgtcgacaaa
      121 gctctcttcc gtgaaagtga attgtttgat gaggagagaa ttctgcgccg ccgcaatgaa
      181 atcgtggacc ggttgcatac cgtcgtcagg ctgcacgatc aggcgaggca taaccggatg
      241 cataggcccc ggatcgtagc accgcgacaa caatgtcggt ttgcgacaga cgggggcttt
      301 gaccgacccg tcgcaagtgc ggagaagcgc cggacaaggc cgcctcggca gaggccgcgc
      361 cttaggccac gcgcctacct cctcaacgga cgttcatcag gcactattgt cggcatggac
      421 agtggcacct ccccacgtgt gctcgtcgtc gacgacgacg ccgacgtgct ggcttcgctg
      481 gagcgcgggt tgaggctgtc ggggtttgac gtggcgacgg ctaccgacgg cgccgaggca
      541 ctgcgcagcg cgaccgagac aagacccgac gcaatcgtgc tcgacatcaa catgccggtg
      601 ctggacggcg tgagtgtggt caccgcgttg cgagcaatgg acaacgacgt gccggtctgt
      661 gtgctgtccg cccgcagctc ggtggacgac cgcgtggccg gcctggaggc cggcgccgac
      721 gactacctgg tcaagccgtt cgtcctggcc gagctggtgg cgcgggtgcg cgcgctgctg
      781 cgccggcgcg gctcgaccgc gacgttcagc tcggagacca tccaggtcgg cccgttggag
      841 gtggacatcc caggccgtcg cgcgcgggtc aacggtgtcg acgtcgacct gaccaaacgc
      901 gagttcgacc tgctggccgt gctcgccgag cacaagaccg cggtgttgtc gcgcgcgcag
      961 ctgctcgagc tggtctgggg gtacgacttc gccgccgaca cgaacgtggt cgacgtgttc
     1021 atcggatact tgcgccgcaa gctcgaggcc cgcggcgcgc cgcggctgct gcacaccgtt
     1081 cgtggagtgg gcttcgtcct gcggacgcag tgatgaacag gctgtcgcgc atctttcgcc
     1141 ggactccgtc tcttcggacg agggtggcgt tcgcgacggc catcggcgcg gcgatcccgg
     1201 tgctcatcgt cggcacggtc gtgtgggtcg gcatcaccaa cgaccgcctc gagcggctcg
     1261 accgccgtct cgacgaggcc gccggttttg cggtgccgtt cgtgccgcgc ggcctggagc
     1321 agatccctcc gtcgccgaac gaccaggacg tcgtcatcac cgtgcgcaag ggtgaccagg
     1381 tgcggtcgaa tgccagcgtc gtgctgcccg aactcgacgc agactatgcc gacaccaacg
     1441 tcgatggggt ccgctaccgg gtccgcacgg tggacatccg cgggtcggag cctccggtgt
     1501 cgttggccgt cggggccacc tacgacgaca ccattgcgga caccaacaat ctgcatcgtc
     1561 gggtgatttt catctgctcg ttcgcgattg tggccgcggg cgtgttcggc tggctgctcg
     1621 cggcgttcgc ggtccgccca ctcaaacggc ttgcccagca agcccgctcg atcgacgccg
     1681 gcgacgaggc gcccgacctg gaaatccgcg gcgccaccga agcggtcgag atcgccgacg
     1741 cggtggaggg catgctgcag cgcatcttca aagagcagga cagaacgaag gccgcgctgg
     1801 cgtcggcacg cgacttcgcc gcggtgtcgt cgcacgaact gcgcacgccg ttgaccgcga
     1861 tgcgcaccaa cctcgaggtg ctggcgacgc tggatatgcc ggatgagcaa cgcaaggaag
     1921 tgctgcacga cgtgatccgc acgcagtcgc ggatcgaggc gacgctgtcg gcgctggaac
     1981 ggctggccca gggcgagctg tcgacgtcgg acgaccacgt gcccgtcgac atcaccgagt
     2041 tgctcgaccg cgccgcccac gacgcgattc gcatctaccc ggatctcgac gtgtcgctga
     2101 tgccgtcacc gtcggtgatc atcgtcgggt tgccggccgg tctgcggctg gccgtcgaca
     2161 atgcgatcgc caacgcggtc aagcacggcg gcgcgacgcg gatccagctg tcggcggtca
     2221 gttcccgcgc cggggttgag atcgcgatcg acgacaacgg caccggcgtt cccgaagagg
     2281 agcgcaagat ggtgttcgcg cggttctccc gaggttcgac ggcgtcgcat tccgggtcgg
     2341 gcctgggctt ggcactggtc gcgcagcaag ccgaattgca cggcggcaca gcgtcattgg
     2401 aagccagccc gctgggcggg gcgcggctgc tgctgcggtt gccgggaccg caaccgcaga
     2461 acgcggaatc tgcgctttag cggctagcgc gtcatcgggt ggtcagcagg tcgatgacga
     2521 aaatcagcgt cttgccggac agccggtggc cgccgccagc ggggccgtag gcctgttccg
     2581 gcgggatggt gagcttgcgg cgcccgccga cccgcattcc cggaatgccg tcctgccagc
     2641 cctggatcag cccgcggagc ggaaattcga tcgactggtg gcgactccac gagctgtcga
     2701 actcctcgcc ggtgtcgtac tcgaccccga cgtagtgcac ttcaaccgtg ccgccgggca
     2761 ccgcttcggc gccgtcgccg acgacgatgt cctcgatgac gagttgtctg ggagccgggc
     2821 cggtcgggaa gtcgatctcg ggtttgtccg tcatcgtcaa cacggtagcg ggcacactgt
     2881 aggcgtggca gaggacaacg aaaccttgac gcggatcaac gcgctggtcg ccgaggagaa
     2941 ggacctgcgc gcgcgactgc agcgcggtga gatcagcggc gacgaggaac actcacgact
     3001 gcgcgacctc gaagcggagc tggaccaatg ctgggacctg ttgcggcagc gtcgtgcgct
     3061 gcgcgaaacc ggcggtgatc ccaaggatgc gcatgagcgg ccggcggacc aggtcgaggg
     3121 ctacctgagc tgacagaggc ctatgacgcc gtcgtggtcg gcggcgggca caacgggctg
     3181 gtcgccgcgg cgtacctggg ccgcgccggc aagcgcgtgc tgctgctgga acgtctccac
     3241 cacgtcggcg gcgcggcggt gtcggcgcag ccgttcgacg gcgtcgatgc ccggctgtcg
     3301 cgctactcgt atctcgtgag cttgttgccg tcgcggattg tcgacgagtt gggcctcccg
     3361 gtgcggctgg cgcggcgtcg ctattcgtcg tacacgcccg acccgtccag cggtggccag
     3421 cgcggcctgc tcgtgggccc ctatcgcggt atcgccgatg acttctaccg ccggtgccgg
     3481 ttggtgactc gggcgctgtg gccgaccgtg accgaaccgt tgttgcggcg ccgtgaggcg
     3541 cggcgcctgg tgaatgccga ggcggcgtgg gtctcgatga tcgacgagcc gatcggacac
     3601 gccatccggg atgcggtgcc cgacgatctg ccgcggggag tgatcgccac cgacgcgctg
     3661 atcgggacgt tcgcgcggtt ggacgaacag acgctgcgcc agaacgtctg tttcctgtac
     3721 cacctgctcg gcgaggattg ggacgtcccg attggcggca tgggcgccgt gacggatgcg
     3781 ttgatcgagg cggcgctgca gtatggcgtc gagatcgtca ccggcgcaca ggttttcgct
     3841 gtcaacccgg atggtgacgt acgttattgc gtcaacgacg acgagcgccg cgtgcacgga
     3901 gagttcgtgc tggccggagt gacgcccacg gtgctgtgct cactcttgga tgagcagccg
     3961 ccgtccgatg gggcgccggg atgccaggtg aaggtgaatc taatgctgcg acgactgccg
     4021 cgactgcggg acaccggcgt cacgaccgag caggcgttcg ggggcacatt ccacgtcaac
     4081 gagacgttca gccaactgga atcggcttac gaaatggcat cccgcggtga ggtacccgat
     4141 ccgctgccgt gcgaaatcta ctgccattcg ctggccgacc cgagcattct gtcgccgtca
     4201 ctgcgggcgt ctggcgcgca gacattgacc gtgttcgggc tgcacacgcc gcacgggctg
     4261 ggcagccgtg agcaactcac cgatgcagtg ctcgattcgc ttaattctgt tttcgcggag
     4321 ccgattcagg cggtactgat gcgaggctgc atcgagacca agaccacata cgacttagaa
     4381 gaatcactcg gcatgacggc gggcaacatc ttccacggcg cactcgaatg gccgttcgcg
     4441 gagaacgacg agccactgga tacgccggcc cgtcgctggg gcgttgctac ttcgcatgag
     4501 cgaatcctgt tgtgcggcgc aggcgctcgc cgcggcggcg gtgtctccgg catcggcggc
     4561 cacaacgccg cgatggccgt gctggaatcc acctgactct tcgcttccgc gagcgtgcgc
     4621 gtctgtaccc cgacacgccg ggaaattacg tacaactacg cacgctcgcg ccgaaactag
     4681 ctaccgctcg ttagtgatct tctcgatgat cgcccgcagt gtgttcaggt cttgctggct
     4741 cagcgccgcc agcgatgcgg gagccgggtc gtcgaccgcc ttcaccagcg cgacgacgtc
     4801 acgaccccgg tcggtgagcg agaccacctt gctgcggcgg tcggtggcat cggtttccct
     4861 taacaccagg ccgcggtccg ccaggtcgtt cacggccacc gtcgcggctg gcgcgtcgat
     4921 ggtggccgcc tcagctatct gcttgatcgt catcggctga cgcgccagcc tgttgaggat
     4981 cctgatcctg ctgaacggca gaccggcctc ggtgaccacc gcccgccgcc aggtgtcctt
     5041 gttgtcaagg acaagacgtg tgaggtctcg ccataccctg tcggccaacc gattacgcgc
     5101 ggatgtacgc atggttctcc tccggctcgg cgatcagcgg cgccagacgc tcggcagaac
     5161 gcagcccccg gtccgacgtc gaatagaaac ccagaaatac gatcaccgca cccagcgcgg
     5221 cgcacacgat ccagagcggc cgggccgcga cggcgaaatc cgcgccggtg gtcgcaagcg
     5281 ccgctcccgc gaccgaaccg cacagcgcca caccgatact cacgcccacc tgccggctcg
     5341 tcgacgcgac cgccgacgcg gcaccagcgc gatccgtcgg catcccgctg accgccgcgg
     5401 tggtgatcgg cgcgttcacc atcgcgaagc cgatgccgaa caccgcgaac agcacgagga
     5461 tttgccagac cggcgtcgct acggtcagct gtgtcagcat gagcgtggcc gtcgtgatca
     5521 gagtcccggc ggccaacagc gacggacggc tgccgaagcg gccgaccagt cggcccgaca
     5581 gcggcgaaaa gatcagtgcg ccaaaggcaa tcggcagata gatcaggcct gtgtgcgcgg
     5641 ccgtgaagtg ccgctcaccc tgcaggtaca gcgacatcat gaacaggaag gcaccgtagg
     5701 cggcgaacgc gcacaccgcg atcatcgtcg ccgacgagaa cggcacgctg cggaagaagc
     5761 gaagatcgat gaacgggtcg tgccgacgag actcgtagcg caggaatccc acgaacgcga
     5821 tcagcgcgag gacggcgacg gcgagggtgc ggggattccc ccatcccatg gtcgggccct
     5881 cgatgagaac gaacacgacg ccgaacagaa acgccatccc caagccttgc ccgacggggt
     5941 cgacgtcgcg catagacgcg gacttggatt ccgggacgaa gatcgcggtc aggatgatcg
     6001 ccgcggcgca gatcggcaga ttgatccaga acaccgcgcg ccagtccatc cactgaatca
     6061 gcccgccacc gaccaatggg ccaagcgcca ttgagatgcc cacgacaccg ccccagatgc
     6121 cgatggctcg ggcgcgttct acccgcccgg taaaggcttg cgtaatgatc gacatggcaa
     6181 cgggattcat catcgatccg ccaatcgcct gtaccaaacg tgcgccgatc agagcatcga
     6241 taccgggcgc gaggctacac agcagcgaac ccagcgcgaa tacggtcaac ccgatctgga
     6301 acgtgcgtcg acggccgaac cgatcggccg tcgcgccgga cagcaacagc agcgacgcga
     6361 ggaccagcgt gtagatgtcg atcatccact gcagctgtga gggggacgcg ccgaagtcgg
     6421 cgcggatgct gggaatcgcg acgttgacga tcgtcgcgtc catcgtgacg atcagcaggc
     6481 tcaggcacaa cgacgaaagg atgatcgcct tccgttgcgg gctgagcgtg ttcatagaac
     6541 aattgtaaaa ctacaactat catgccgcaa agtgagatgc accactgccc tcccgcgaga
     6601 ctcccgtttc gcagacaaag tgcgagagcg cgcgtgcgaa acggaagttt cggcgcaagg
     6661 gctgcaacta gctcagcgag ctacctgctg tcgatggcct tgtagtcgcg ctcgccgtag
     6721 ccggtgtaga tctggcgcgg ccggccgatc ttgccgccgc gctcgtcgtg catctcacgc
     6781 cagtgcgcga tccagcccgg cagccgcccg agcgcgaaca gcacggtgaa catcggggtc
     6841 gggaagccca tcgcgcggta gatcacgccg gtgtagaagt cgacgttcgg gtacagcttg
     6901 cgctcgacga agaagtcgtc ggtgagcgcc acttcttcga gcgccttggc gatgtccagc
     6961 agctcgtcgt cgccgccgag cttgccgagg atcttgtcgg cctgctcctt gacgatgcgc
     7021 gcacgagggt cgtagttctt gtagacccgg tggccgaagc ccatcagctt gacgttgtcc
     7081 tcgcggttct tcaccttctt gacgaagtcg tgcacgtcgc cgtcggcctt gcggatcttc
     7141 tccagcatct ccagcaccgc ctggttcgcg ccgccgtgca gcgggcccca cagcgcgttg
     7201 atgccaccgg agatcgacgt gaacaagttg gcctgcgacg agccgaccag ccgcaccgtg
     7261 gacgtcgagc agttctgctc gtggtcggcg tgcaggatga acagcatgtc gagcgcccgc
     7321 acgacctccg ggtcgacctc gtacggttcg gccggaaacc cgaacgtcat ccgcaggaag
     7381 ttctccacca acgtcagcga gttgtcgggg tacaagaacg gctgaccggc cgacttcttg
     7441 taggcgtagg cggcgatggt tggcagcttg gccagcaacc ggatcgtgga cagctccacc
     7501 tgtccgtcgt cgaacggatc caacgagtcc tggtagtacg cgctcaatgc gttgactgcg
     7561 ctggacagca ccggcatcgg gtgagcgttg cgggggaacc cgtcgaagaa ccgcttcaag
     7621 tcctcgtgca gcaacgtgtg ccgttggatc tgggtggtga acttggccag ctgctccttg
     7681 gtcggcaact cgccgtagat cagcagatag ctcacctcga tgaacgtcga cttctcggcc
     7741 agctgctcga tcgggtagcc gcggtaccgc agaatcccgg cgtcaccgtc gatgtagcag
     7801 atcgcgctct tggtcgatgc ggtgttgacg aagcctgggt cgaaggtcgt gtaaccggac
     7861 ttcgacaaaa ggttgcccag agcgatgccg tcggcgccct cggtggcgtg agcgatctcc
     7921 agctcgagct cacccccggg gtattgaagg gtggcgaact cttcagcgtt ggatgagtta
     7981 tcggccacag atgtcccttc atcgctggcg agggtgagtt gtgcctacca aacggtagtc
     8041 gtattcgcgg gggcatgccc gcggggggta gatgagcagg ttaggtgacg ctggcgggtg
     8101 gggagcagat atcggtgaac tgcttgtagg tgtcattcag tcgggcgacc agcatgtcgg
     8161 tgtcgacgtt ttgccagtcg gtatccggtc cgagatcgcc gaacgcggtg agcaggatca
     8221 ccgaccacac cgcgaccgcc attaccacct tcgggtcgtc gaccgcgacg cccatccgca
     8281 tggccaacgt gtgagtcacc gagtgctcac ggaactcacc ggacgccgtg cgcagcgtcg
     8341 gcgaggagtt cacgatgcgt gcgagcgtga acatccgcgc ggtggtgaag ccgccgggcg
     8401 gggccgacac cgtcgcccgg atcattgcgc tgtgcgcggc cagcaacgct tccatggggc
     8461 agagttcggt cggctggcgc tccagttgga cggtgagcgc gtcactcatg tcgtcgatga
     8521 gcgccatgat gacggcgtcc ttggtggcga agtagcggct gaacgtgcgc ggtgagacgt
     8581 cagcgaccgc ggcgatctgg tcgacggtcg tgttgtcata cccctgctgc tcacacagtt
     8641 gcaccgccgc gtcgatcagc gtcgaccgcg tgcgctgctt cttgcgttca cgcaggcctg
     8701 gaatccccgc cccgacggcc tccaccacga acaggatcgt aacccgcaga ttcgagctgg
     8761 ttgtcgagat cagctagctg attgcggcgg ccgaaacccg gtatcggacg aacgccggca
     8821 ccgccacggc ggcgatcagc accccgacga ccaccagtgc cccgccgccc gccgcggcga
     8881 tcgtggtgcc gacggccgca gcggcggcac cgtgcgcggc gtcggccaac cgcgggccgc
     8941 cggcgacgac gacgatgaac acgccctgca accggccccg cacgtcgtcg gacgccgcct
     9001 gctgcaggat cgtcgaccgg aacgccgccg ataccatgtc cgccgcgccg ccgaccgcca
     9061 ggaacgccag cgacgcccac aacaccgcgc ccgcccggcc tcccgcgatt ccgctgcaca
     9121 gcccgaagcc gaccatcgcg agcccccaca ccacgatcgc ggcgaccacc accaggcctt
     9181 gccggcggat ccgcggcagc cagccggaga acaccccgcc cgcaacggcg cccgcagaga
     9241 tcgcggccgc cagcaacgcc atcgtggtgc cgccctcaac cgggccgccg aaactctcgt
     9301 gggcgatctg cgggaacagc gcccgtggca tgccgaagat catggcgatc aggtcgacga
     9361 caaacgacat caacaccacg gtgttgccgg ccagataacg gaacccgtcc cacaccgaag
     9421 cgagcccttt gcgcgcggta ccgtccccgg tcgggggcat cgacgacaag cggatggtcg
     9481 cccagatcgg cgcgacacag gtgatcgcgt cgatcagata cagcgtcgac aggtcgaccc
     9541 agcgcagcat cacgccggcg agcagcggcc cgacgattgc cccgaactgc gtgacggtca
     9601 tgttcagcga gttcgcggcg ggaagctgat cgtcgggcag catgcgcggg atcgccgccg
     9661 agcgagtcgg agcgttgatc gcgtagaacg cctgctgcac cgacagcaag cacagcacca
     9721 gccagacgtt gttgagcgcc agcgccgcct gcacccacag cagcaccgac gccagcgcca
     9781 atccgcacga cgcgatgatc agcagaacgc gccggtccat cgcgtccgcc cacgcgccgc
     9841 cccacagccc gaacacgatc aacggaacca acgcgaacac accggacagg ccgacgtacg
     9901 cggagctttg ggtgagcgca tagatctgga ccgggacggc gaagatcgtc aggttcgcgc
     9961 cgatcaccgt gacgacgccg gacagccaca gccgacggaa gtcgggtgtc cgcagcggtg
    10021 cggtgtcggc gagcaggctc acggctcagg gttgaaggcg ctcaacacgt cccgcttgca
    10081 agcggatcct gttgtgcagc cggtcctcgc ggccctgcca gaactcgacg acctcggcgg
    10141 cgatgaggta gccgccccaa ttcggcggga gcgggacctc ctcgacgtcg gcgaaccgct
    10201 cagtgacctt ctgaagttgt gccaacagct cggctcgcga cccgatcggc tgcgactgcg
    10261 ccgacgccca cgcgcccagc tgtgaaccgc gcggccgctt cgaccagtag tcggcggtgg
    10321 cctccgcggt gactttgctc acagcgccac ggatgtgcac ctgccgaccc agcgcgaacc
    10381 acggaaacgt cgcggacgcg taggggttcg cggcgagttc ggcgcccttg gcggagtcgt
    10441 agttcgtgaa aaacgtgatc ccggtgtcgt ccacgctctt gcacaacacc gaccggctca
    10501 tcggccttcc ctcggcgtcg acagtggcca ccaccatcgc gttcggctcg gcgacaccgg
    10561 cgcggtcggc gtcgtcaatc catttgcgca gcaacataac ccagccatcg gcaagccaat
    10621 cgacgtcgag gtcggggctg ccgtccttct ccaccgatcc gtagtccact cgcatgttcg
    10681 ccaggtgctt gtcgccgggt tgcgccacga gccaacgcta cgccggtgcg tcggcgccga
    10741 gttacccacc ggtagtaact gtgtccgcgg cgggtgcgag aatcaccgca tgactgcggt
    10801 accggagaat ttcgtcagcg gacttgaggg cgtcgttgcc ttcaccaccg agattgccga
    10861 gccggacaaa gacggcggcg cgctgcgcta tcgcggagtc gacatcgagg agctggtcga
    10921 gcagcgggtc acgttcgccg acgtgtgggc gctgctggtg gacggcaagt tcggtcagag
    10981 cttgcggccg gcggagccat tcccactgcc catccacacc ggtgacgtgc gcgtcgacgt
    11041 gcaggccggg ctggcgatgc tcgcaccgat ctggggttac aaaccgattc tggacatcga
    11101 cgaccagacg gcccgcgagc agttggcccg ggcctcggtg atggcgctgt cgtatgtcgc
    11161 gcaatccgca cgtggcatcg agcgtcctgc ggttccgcag cgcgaaatcg accaatgccg
    11221 aactgtcaca gagcgtttca tgaagcgatg gaagggtgac cccgatccga agcaggtcga
    11281 ggccatcgac gcgtactggg tgtcggccgc cgagcacggg atgaacgcgt cgacgttcac
    11341 cgcgcgggtg atcgcgtcga cgggtgccga cgtcgcggcc gcgctgtccg gggcgatcgg
    11401 cgcgatgagc ggcccgctgc acggcggcgc gccggcccgc gtgatcccga tgatcgagga
    11461 ggcggagagc accggcgacg cgcaggcggt ggtcaagggc atcctcgacc acaatgagaa
    11521 gctgatgggc ttcggccacc gggtgtatcg cgccgaggac ccccgggccc gggtgctgcg
    11581 ggcggcggcc aagcgcctgg acgcgccgcg ctacgaagtg gccgccgcgt tggagcaggc
    11641 ggcgttgacc gagctgcggg agcgtcgacc cgaccgcgcg atcgagacca acgtcgagtt
    11701 ctgggcggca atcatcctcg acttcgcggg ggtgccgccc aagatgatgc cggcgatgtt
    11761 cacctgtggg cgcaccgccg ggtggtgcgc ccacatcctg gaacagaagc ggctcggcaa
    11821 gctggtgcgg ccgtcggcgg tgtacgtcgg acccggcccg cgttcggtcg agtcggtgga
    11881 cgggtggtcg gagatttcgc acgccgcggc ctgagcccgg tcgactacat gagctgccgc
    11941 tggaagtagg tgtattcgag cgcggccgtt cgggcctttt cgtcgatgtt ggtcccggct
    12001 ccgtggccgc cctgcgtggc ttcgtagaag aggtagggga ttccgaactc ggacaggcgc
    12061 gccgcgaact tccgagcgtg ctgcgggccg acccggtcgt ctttactggt ggtgaagatg
    12121 aacggttccg gatacttcac gccgcgcttc aattgtgcgt agggcgagat tgattcgaga
    12181 aattcacgct cgtccggatt cgccaccgag ccgtattcgc ccacccacga cgcgcccgcc
    12241 gcaatctgct cgtagcgcac catgtccagc agcggaatct gcatgtccac cgcgttccac
    12301 agctccgggt gctgggtgaa ctcaacgccc atgagcagac cgccattgga gccgccctgg
    12361 atgccgagat ggcgcggact ggtgatcttg cgggcgatca gatcgcgggc tacggcggtg
    12421 aaatcgtcga acgccttctg ccgcttggtt ttcaacgccg cctcatgcca ggccggaccg
    12481 tattcgccgc cgccgcggat attggcgatg acgtaggcgc cgccgcgttc cagccagagc
    12541 ttgcccatca ggccgttgta gcctggcgtc gaggacgact cgaaaccgcc gtatgcgtac
    12601 aagatcgtcg gcgtggtgcc gtcgtacttc aagtcggcgg cgtgcacgat gaagtacggc
    12661 acctcggtgc cgtcgggtga ggttgccttc agctgctcca ccacgaagcg cgacgaatca
    12721 aaccgcgccg gagaggattt caccggatcg acgcggccgt cggcggtatt caggtggtac
    12781 agagtggtcg gggtgagcat cgaggtgacc gagagatagg cgacatcgcc gcgtgaatcg
    12841 gcggccaccg cactgaccgt cgcattgtcc ggcaagggaa ccggctgccc cgaccaggtg
    12901 ccgtcgggcc cccggctgta cacggtggcg cgtcccttga cgtcgtacag cgaggtgacg
    12961 acgacgcggt cgcgcgtggt catcacgctc tgcatcgcct cctgcggccc gggcgcgtag
    13021 acgacggtgg ccctcagccg ctctggatcc agcgtcatct cgtcgacgtt cagcgatacc
    13081 agcgaccctg agtggaaggt ggtgccgccg gttgtccagt cctgccgaag cgagaccagc
    13141 acctggtcgc cgaccatgcc ctccacgtcg gccttgggcg gcaacgccat tcgcaccggc
    13201 tgcccaccgc ccagcagact gacctggttc tcgtagaacg tcggccggcg caccaccaga
    13261 ttcagcagcc ggcccgcgcc gttgtccagt agcgcgccgg tggtggaaag ctcgtcggcc
    13321 ttgtcgccgc gcgcgacctc caccgcctgg tccaggggct gcccgcgctg ccaccgtttg
    13381 accacgtacg gatagccgga ggtcgtcatc tcccccggct gccattcccg tgacaccaac
    13441 agcgtgtcgg cgtccaccca cgtcgtgtac tgcttgccgc gctccaacac gaacccgtcg
    13501 ggcacgaatt gccctgccgc acggtcgaat tcgcgcacag ccacggcgtc ctcgccgccc
    13561 tcggacagac tcaccagaca gcgcatgcgc gtcaccggat cacaatccat cccctcccac
    13621 acccaattgc gcccctcggc ctgtgcgagc gcgtccagat ccagcaccgt cttccactgc
    13681 ggctgcggcg actcgtaatc gatgacggtc gtctcccgcc agatcccccg cttgtgctgt
    13741 gcgtcttgcc agaagttccc gacgaccccg tcttccagca gagccggaag cggcaaccgg
    13801 tccggcgcgt tccccagttc caccgcctgc gccaggttgc ccgcgtaccg cggatcctgc
    13861 tccagcacgg cgagcgtctt cgcgttctcc gcctggaccc acgactgcac ctgaggactg
    13921 tccaactcct ccagccacag gtacggatcg gtcgccgtgt cggatctgct gcacgctgcg
    13981 accaggacgg ccaacgtcac gatgaccgcc gttcgtgctc ggcccatgtc tgtcacccgt
    14041 tggtctgcgc ctcgatcgag gagtcgtgtg cacgttacat ccacttgtcg gtgcccggtg
    14101 gttgcatggc ggtgtagcga tgagttcggc gtccgcggcc ggtcgatatc actgaccccg
    14161 ccgtggggtc cgacatctac gaaggagatc accatggcaa tcgaagtgac gcccgctgtg
    14221 ctaccccacc tgacggtcga caacgccgcc gccgccatcg acttctacgt caaggcattc
    14281 gacggtgttg agctgggccg ggttccaggc cccgacggca agaagctgat ccacgcggcc
    14341 gtgcaggtca acggcaccac agtgatgctc gccgacgact tcccggagat gaacgacggc
    14401 aagtccacca caccgaacgc gctcggcggc acgcccgtga ccatccatct cgtcgtgacc
    14461 gacgtcgaca cgcagttcca acgcgcggtg gacgcgggcg ccacggtcgt gatgcctgtg
    14521 gaggagcagt tctggggcga ccgctacggc gtgctgcgtg acccgtttgg gcaccagtgg
    14581 tcaatgggtc agccggtgcg cgaggtgagc cccgaggaga tcgcgaacgc gatgcagcag
    14641 cagtgagcgc ttcgggcgcc tgctgacgac ttcggagcag cgcatagcat cgcggttgat
    14701 ggcatcacct gatcacaacg agcccgccga ccagccggcg aattcgtctg accagaagcc
    14761 ctcccgcgta cgcgcggtga ggtctgcggt ggtcaggccg ttcacgcgga cgggtcggta
    14821 ctacgcgcag tcgcggcggg atttccgcga ccgcatagcg accgaacttc ctctcgtgcg
    14881 gcccacgctc ggcctcctcg cacaggcgtt ccgcgatgag gtcgtgcttg tcgggctgca
    14941 gctgagccgg ccgatcagcg aaccgcaggc gttcgagcgg atcacccgcg aggtggaggc
    15001 cgcgctcgag atgtatgggc gaaatggctg gctggacaag ccggagacgt atttcgccgc
    15061 gccgcggccg ctcactaaca tcacgatcca cgatgtcaac cgccgcaagc ggtcctacca
    15121 gcggttcgtg ttcgacagcg gctatgcgcc gtatccgggt gagccgggcc gtgagcggtg
    15181 gctcagctat gaggcgacca agcgcgtgta cgggttgatg ctgcgccatc gcgaaccccg
    15241 gccgtgggtg gtgtgcgtgc acggcacgga aatggggcgg gcccgtgtcg acctgaggct
    15301 cttccgagct tggcatctgc acaacgatct cggcctgaac gtcgccctgc ccgtgctgcc
    15361 gctgcacggc ccgcgcaagc ggggagtgcc gaagggtgcg gtgttgcccg gtgaggatgt
    15421 gctggacgac gtacacgcgg ctgctcatgc cgtgtgggac atccgccgcg tggtgtcgtg
    15481 gatccggtcg caggagccgg agtcggcaat cggcctgaac ggcatgtcgc tcggcggcta
    15541 catcgcgtct ctcgtggcca gtctcgagga cggcctcact tgcgcgatcc tcggcgttcc
    15601 cgtcgccgat ctggtcgaaa tcctcgcgcg ccactctggc ttgagcagcg acgacccgcg
    15661 ccgggagacg atcacgatgg ccagacccct gggccgcatg gtgtcaccgc tttcgctcac
    15721 cccgcgcgtc ccgatgaacg gccgcttcat ctacggcgga gtggccgacc aggtcgtgca
    15781 ccctcgggat caggtggtcc gcttgtggga acactggggc aaacctgaaa tcgtctggta
    15841 ccgaggcggt cacgcgggtt ttttccgctc ccggcccgtg caggacttca tcgacgccgc
    15901 actcgtgcag tcgggactcc tccattcgtg acgaattaac gcttcagcaa cgacagcacg
    15961 ccgcgccggt ctgtcgttgt gggcggccag catctcgcca atggtggtga acttgtcgcg
    16021 cggtcttcct tcggcgcggc cgcggtcgat ctcggcccgg tcgatggcgc gccagcgcgc
    16081 atggtcgacg acgtcgggct gtcggcgacg aaccagtctg tccagcgcgt gcagtcccgc
    16141 cgccggatcg gcgagccgac cgtcgttgta atcgtcgacg agcgcattga cggtttcctg
    16201 agcacacgac ttgttggtgc cgatgaaacc cgttgggccc cttttgatcc agccagtgac
    16261 gtacgaaccg cggactggag caccggtcga cgggtcgacc gcccggccgc cgcggttggg
    16321 caccacatcg gcgtcgtcgt cgaatggcag gccgggaacc ggccagccgc ggtagccgat
    16381 tgaactcagc accagtcccg cctcgacctc gagcgtctcg tcggtacccg tcacggtgaa
    16441 ctcgacaccg gcagcgcgtt cgttcccaag cacccgcgac ggggtcaggc gatacgcgag
    16501 ccggatcacc ggcgcgtcgt cgcgttcgtc gtaaatatcc agcgacgcaa gcagttccag
    16561 cttgtggcgg gacatcctgt cggtgcagtc tgcgaggtcc gcatgcacca gctcgtggtt
    16621 gtcggcgctc agcaccaccc gcgacgcggc cgtcagcccg atcatctccg gaagtgtgaa
    16681 cgccgactga gcgggccggc ggcgggcggc gacgacctcg cgaaccttgc tgtcgcgcag
    16741 cgcggccagc gcgtgccgcg aaatatcgct gtgagccaac cgtttcgggt cggcggtcag
    16801 gatccgcgcg acgtccagcg cgacgttgcc gttgccgacg atcaccaccc gctcgtggtc
    16861 gagtggcacg gcgaggtctc ggtagtcggg gtgagcgttg atccaggcga cgagttcg
//
LOCUS       DASWBF010000565         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_848951,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000565 DASWBF010000000
VERSION     DASWBF010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848951"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VF874_21205"
     CDS             <1..578
                     /locus_tag="VF874_21205"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9178487.1"
                     /translation="QPLYDYGVKHPGWVQFWDVLCTVFSPAAFRLVGAAIVVLAVFRR
                     NLRVILFILTAIGMSGIVTQIAKGLADRPRPTTALAAASSSAFPSGHALGVMAAVLAL
                     LTISAGMFNRRGRAASIVFGAVVVIAVGVGRVVLNVHHPSDVLAGWALGYLWFLACLL
                     AIRPLPLAAVAAPEVEPIGAAADETPQARGI"
     gene            complement(532..>1599)
                     /locus_tag="VF874_21210"
     CDS             complement(532..>1599)
                     /locus_tag="VF874_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9178488.1"
                     /translation="LGLVLALLAGLVGLSRVATGAHYPGDVFIGFGIGASIAVLGGRL
                     VPPIVTSAIPKADPLRVYTPPRPDGDGVVMVVNPASGRGSDSVIAQVREQLPRAEIVE
                     LAPDDSVEETARKAAERARVLAVCGGDGSVSCVASIAVEAGLPLAVFPGGTFNHFAKD
                     IGCDSAAKTIQAIHDGTVFRVDLVCFNGDNIVINTASIGAYPTFVQTRERLEHKISKP
                     VATLYSMVHTLRHGEPVRIQFDNKTMQTSLFFIGNSTYLPSGFAPSRRTRMDDGLLDV
                     RILETGRRLSKLRILASLMLGRLQRSALYHERQVPEFAFKALDGPTMIARDGEVGSEH
                     ESASFSVRYRALAVFRLPPRR"
BASE COUNT          244 a          533 c          543 g          279 t
ORIGIN      
        1 tgcagccgtt gtacgactac ggcgtgaaac accccggctg ggtgcagttt tgggacgtgc
       61 tgtgcaccgt attcagcccg gcggcctttc gcctcgtcgg tgcagcgatc gtggtcctcg
      121 cggtgttcag acgcaatctg cgggtcatcc tgtttatcct cacggcgatc gggatgagcg
      181 ggatcgtcac acagattgcg aaaggcctgg ccgaccgccc ccggcctaca accgcattgg
      241 ctgcggcaag ctccagcgcg ttcccgtcgg gccatgcgct cggcgtgatg gcggccgtcc
      301 ttgcgctgct taccatttcg gcgggcatgt tcaaccggcg cggacgcgcg gcatccatcg
      361 ttttcggcgc ggtggtggtg atcgctgtcg gcgtcggccg cgtcgtgctg aacgtgcacc
      421 acccgtccga cgtgctcgcc ggctgggcgc tcggctacct ctggttcctt gcctgcctgc
      481 ttgccatccg cccgctgccg ctcgccgcgg tggccgcgcc cgaggtggaa cctatcggcg
      541 cggcggcaga cgaaacaccg caagcgcgcg gtatctgacg ctgaagctcg ccgactcgtg
      601 ctcggatccc acttcgccgt cgcgagcgat catggtcggg ccgtccagtg ctttgaaagc
      661 gaactccggc acctggcgtt cgtggtagag cgcgctgcgc tggaggcggc ccagcatcag
      721 cgacgccaga atgcgcagtt tgctcaagcg gcggccggtc tccaggatgc gcacgtcgag
      781 cagcccgtcg tccatgcggg tgcgccggga gggtgcgaag ccggacggca ggtaggtgga
      841 attcccgatg aagaacagcg acgtctgcat cgtcttgttg tcgaactgga ttcgtaccgg
      901 ctcgccgtga cgcagcgtgt ggaccatcga atacaacgtc gcaaccggct tgctgatctt
      961 gtgctccagc cgttcccggg tttggacaaa cgtcggatag gcgccgatgc tcgcggtgtt
     1021 gatcacgatg ttgtcgccgt tgaagcagac caggtcgacg cgaaagacgg tgccgtcgtg
     1081 gatcgcctgg attgtcttgg cggccgagtc gcagccgatg tcctttgcga agtggttgaa
     1141 cgtgccgccg gggaacaccg cgagaggcaa cccggcctcg acggcgatgc tggccacgca
     1201 cgacaccgag ccgtcaccgc cgcagacggc aagcacccgc gctctttcgg ccgccttccg
     1261 cgccgtctcc tcgacggagt cgtcgggcgc caactcgacg atctcggccc tcggcaattg
     1321 ctcacggacc tgcgcgatga cgctgtctga accccggccg gacgccgggt tcaccaccat
     1381 caccactccg tctccgtcgg gtcggggcgg cgtgtagacc cgcaacgggt cggctttggg
     1441 gatggcggag gtgacgatcg gcggcaccag ccgaccgccg agcacggcga tcgacgcgcc
     1501 gatgccgaag ccgatgaaga cgtcgccggg gtagtgggcg ccggtcgcga cgcgggacaa
     1561 cccgacgagc ccggcgagca gcgccagcac cagccccag
//
LOCUS       DASWBF010000566         4358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1248581,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000566 DASWBF010000000
VERSION     DASWBF010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248581"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1055)
                     /locus_tag="VF874_21215"
     CDS             complement(60..1055)
                     /locus_tag="VF874_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX9178489.1"
                     /translation="MHGQANVRGRVWRAGKPCDDFEFDKIADYLAEDDTLVWVDMFNP
                     DHAALCDLAQELGLNDWAVEDAVAARERVKATVYATHTFFTVYAVQVLDTGVDSETVL
                     SMHRISAFVLPQGLITVRLAPDFDIDDVPKRWDELGGQEYGVGSLVHGLLDVVVDGHF
                     EAVEALDDYIESIEDDLFDGNASKRGFQRRTYQMRRDLVHLRRVALPMREVVNAIQHH
                     RVNVKSARELDPHYDDLYDHVLRVSEWTESLRDMITTVFETNLSLQDARLNTVMKKLT
                     GWAAIIAVPTAITGFYGQNVPYPGFGAWSGFVVSTAVIVLLVVVLYVAFKRRGWL"
     gene            complement(1170..2090)
                     /locus_tag="VF874_21220"
     CDS             complement(1170..2090)
                     /locus_tag="VF874_21220"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX9178490.1"
                     /translation="MALRPSGSSHPFDASLWHPIDGFGDLTDITYHRHVSDGTVRVAF
                     NRPEVRNAFRPHTVDELYRALDHARMSPDVGVVLLTGNGPSPKDGVWAFCAGGDQRIR
                     GRSGYQYASGVTAETVDPARAGRLHILEVQRLIRFMPKVVICLVNGWAAGGGHSLHAV
                     CDLTLASREHARFKQTDADVGSFDGGYGSAYLAKQVGQKFAREIFFLGRPYTAEQMHR
                     MGAVNEVVDHADLETVGVEWAKTINAKSPQAQRMLKFAFNLLDDGLVGQQLFAGEATR
                     LAYMTDEAVEGRDAFLEKRDPDWSPFPRYF"
     gene            complement(2102..2740)
                     /locus_tag="VF874_21225"
     CDS             complement(2102..2740)
                     /locus_tag="VF874_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178491.1"
                     /translation="MIARLLLATAVAFALVPAVTAAPASAASCSQTKPPLDAASRQVG
                     RGDMLWVGRDGSVGITTPAGTGVVQVANAGPLPLQAMLVDAQQNGQHQLIVSNGRGAH
                     LYLVSGCDITPVVDDQGEPFVFDQENLRGAGTGIGCADLGDGRHLVGLQAQIGDSGWT
                     VRRTEIVVNGATATPGRSDTVAAPSEQDPAVTAAQTITCGDQTITKDGVQQP"
     gene            complement(2737..3201)
                     /locus_tag="VF874_21230"
     CDS             complement(2737..3201)
                     /locus_tag="VF874_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX9178492.1"
                     /translation="MSNTERIRPPWWLKPMNKVFIAMMRLGLPISGKESPTVLTAPGR
                     KSGKPRSTPVTPMHVDGHRYAVEGFPGADWVANVRAAGEATLTQGRRSERVRMVEMSP
                     DEARPLLREFPTEVPTGVEFMKKAGLVKDGRPEEFEALAGRCAVFRFDPVAS"
     gene            complement(3219..3896)
                     /locus_tag="VF874_21235"
     CDS             complement(3219..3896)
                     /locus_tag="VF874_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9178493.1"
                     /translation="MVAAVLFDFSGTLFRLEEDDSWFAGMQLDHDAEVDGHVQAELMR
                     RLTAPTGRSVEMTPDKYRTWVNRDLAPHLHREAYLHVLRASGLADHHAEALYRRVIDP
                     SCWTPYPDTASVLKALSAKGIKTAVVSNIAFDLRPAFEALGADVDEFVLSFEIGAVKP
                     DPAIFQTALERLGVKACDTVMVGDSDDADGGARDVGCGFVLVDPLPTAHRPDGLLQAL
                     ASYGVDL"
     gene            complement(4128..>4358)
                     /locus_tag="VF874_21240"
     CDS             complement(4128..>4358)
                     /locus_tag="VF874_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215065.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9178494.1"
                     /translation="EAVTAVVVLREDAPRDQESVTAMTVEIQAAVKERKGSVQSPKQV
                     VVVDALPLTGLGKPDKKAVRAKFWKGAGRAVG"
BASE COUNT          700 a         1509 c         1425 g          724 t
ORIGIN      
        1 agtgatcatc cactcggcag cctcgtgcgg ggtcagcgcg ggcaatccct ggtaggtggc
       61 tacagccaac cgcggcgctt gaacgccacg tacaagacca cgacaagcag gacgatcacg
      121 gcggtgctga cgacgaaccc cgaccaggca ccgaagcccg gatagggcac gttctggccg
      181 tagaagccgg tgatcgccgt cggcacggcg atgatcgcgg cccagccggt cagcttcttc
      241 atcacggtgt tgagccgggc atcctgtagc gacagattgg tctcgaaaac cgtagtgatc
      301 atgtcgcgca acgattccgt ccattcggat acccgcagga cgtggtcgta cagatcgtcg
      361 tagtgagggt cgagttcccg tgcggacttg acgttgacgc ggtggtgctg gatcgcgttg
      421 acgacctcgc gcatcggaag cgcgacgcgg cgcagatgga caagatctct gcgcatctgg
      481 taggtccggc gctgaaagcc ccgcttactc gcgttcccgt cgaacagatc gtcttcgatc
      541 gactcgatgt agtcgtcgag tgcttccacg gcctcgaagt gtccgtcgac cacgacgtcc
      601 aacagcccgt gcacgagtga gccgaccccg tactcctgtc ctccgagctc gtcccacctc
      661 ttgggcacgt cgtcgatgtc gaaatcgggg gccagccgca ccgtgatcag accctgcggc
      721 agcacgaacg ccgagatccg gtgcatcgac aggacggtct ccgagtcgac gccggtgtcg
      781 aggacctgga ccgcgtaaac ggtgaagaac gtgtgggtgg cgtacaccgt tgccttcacc
      841 cgttctcgcg ctgccacggc gtcttccacc gcccagtcgt tgagcccgag ctcctgagcc
      901 agatcgcaga gtgcggcgtg gtcggggttg aacatgtcga cccacaccag cgtgtcgtct
      961 tcggcgaggt agtccgcgat cttgtcgaac tcgaagtcgt cgcaaggttt gcccgcccgc
     1021 caaacccggc cacggacatt ggcttgccca tgcaccacac catcatctca atgtcgacgg
     1081 cccgaacgca tatcggttcg ccggaatcac gaccgaggca tgagcgctca agcgcaacga
     1141 agggcggcga cgccggacga gcccgctgct cagaagtagc gggggaacgg gctccagtcc
     1201 gggtcgcgct tttccaggaa cgcgtcgcgg ccctcgacgg cctcgtcggt catgtacgcc
     1261 agccgcgtcg cctcaccggc gaacagctgc tggcccacca acccgtcgtc gagcagattg
     1321 aacgcgaact tcaacatccg ttgcgcctga ggcgatttcg cgttgatggt cttcgcccac
     1381 tccaccccga cggtttccag atccgcgtgg tcgacgacct cgttgaccgc acccatccga
     1441 tgcatctgct cggcggtgta cggccggccg aggaagaaga tctcgcgggc gaacttctgc
     1501 ccgacctgct tggccagata cgcgctgccg tagccgccgt cgaagctgcc gacgtcggcg
     1561 tcggtctgtt tgaagcgcgc gtgctcgcgg ctggccagcg tcaggtcgca caccgcgtgc
     1621 aggctgtgcc cgccgccggc cgcccagccg ttgacgaggc agatcaccac cttcggcatg
     1681 aaccggatca gccgctgcac ctcgaggatg tgcagccgcc cggcgcgggc gggatccacc
     1741 gtctcagcgg tcacgccgga cgcgtactgg tagccgctgc ggccgcggat gcgctggtcg
     1801 ccgccggcgc agaacgccca gacgccgtcc ttcggggacg gcccgttgcc ggtcagcaac
     1861 accacgccga cgtcgggtga catccgcgca tggtccagcg cacggtacag ctcgtcgacg
     1921 gtgtgcggcc ggaacgcgtt gcgcacctcc ggtcggttga acgccacccg caccgtgccg
     1981 tcggacacgt ggcggtggta cgtgatgtcg gtgaggtcac cgaagccgtc gataggatgc
     2041 cacagcgacg cgtcaaaagg gtgcgatgag ccgctcgggc gaagagccat gtcattgtcg
     2101 gtcaaggctg ttggaccccg tccttggtga tcgtctggtc gccgcaggta atcgtctgcg
     2161 ccgcggtcac cgccgggtct tgctccgagg gtgcggcgac ggtgtcggac cggccgggtg
     2221 ttgcggtcgc gccgttgacg acgatctcgg tgcggcgcac cgtccagccg ctgtcgccga
     2281 tctgcgcctg caacccgacc aggtggcggc cgtcgccgag gtcggcgcac ccgatgccgg
     2341 tgcccgcgcc acgcaggttc tcctgatcga acacgaacgg ctcgccctgg tcatcgacga
     2401 cgggagtgat gtcgcaaccg gacaccagat agaggtgagc gccgcggccg ttgctgacga
     2461 tcagttgatg ttggccgttc tgttgggcgt cgaccagcat ggcctgcagc ggcagcgggc
     2521 cggcgttggc cacctgcacg acgccggttc cggcgggggt ggtgatgccg acggaaccgt
     2581 cgcggccgac ccacagcatg tcgccgcggc cgacctgtcg gctggccgcg tcgagcggcg
     2641 gtttggtctg gctgcacgag gcggccgacg cgggcgccgc cgtcaccgcg ggcacaaggg
     2701 cgaaggccac ggccgtcgca agaagcaggc gggcgatcat gatgcgaccg ggtcgaatcg
     2761 gaacaccgcg caccggccgg cgagcgcctc gaactcctcc ggccggccgt ctttcaccag
     2821 gcctgccttc ttcatgaact ccacgcccgt cgggacctcc gtcgggaact cccgcagcag
     2881 tggccgcgcc tcgtcgggcg acatctcgac catccgcacc cgctcggagc ggcggccctg
     2941 cgtcagcgtc gcctcgccgg ccgcgcgcac gttagcgacc cagtccgcac cggggaagcc
     3001 ttcgacggca tagcggtggc cgtcgacgtg catcggtgtg accggggtgg agcggggctt
     3061 gcccgacttg cggcccggcg cggtcagcac ggtcggactt tccttgccgc tgatcggcag
     3121 ccccagccgc atcatcgcga tgaagacctt gttcatgggt ttcagccacc acggcggccg
     3181 gatgcgctct gtgttggaca tatcggcgac gctacctgtc acaggtcgac gccgtaactg
     3241 gccagcgcct ggagcagccc gtccggccgg tgcgcggtgg gcagcgggtc gacgagcacg
     3301 aaaccgcacc cgacgtcgcg cgccccgccg tcggcgtcgt cgctgtcgcc gaccatcacg
     3361 gtgtcgcagg ctttgacacc cagccgctcc agcgctgtct ggaagatcgc cgggtcgggt
     3421 ttgacggcac cgatttcgaa cgacagcacg aactcgtcga cgtcggcacc gagcgcttcg
     3481 aacgccggcc gcagatcgaa cgcgatgttg gacacgacag cagtcttgat tcccttggcg
     3541 gacaacgctt tcaacacgga agcagtgtcc gggtacggtg tccagcagga tgggtcgatg
     3601 actcggcggt agagggcctc ggcgtggtgg tcggccaggc ccgacgcccg cagcacatgc
     3661 aggtaggcct cccggtgcag gtgcggcgcc aggtcgcggt tcacccacgt gcggtacttg
     3721 tccggcgtca tctcgaccga ccggccggtg ggggcggtca gcctgcgcat caactccgcc
     3781 tggacgtgcc cgtccacctc ggcgtcatgg tccagctgca tcccggcgaa ccagctgtcg
     3841 tcttcctcga gccgaaacag cgtgccggag aagtcgaaca acaccgccgc caccatgcgc
     3901 ccatcgtgcc agcggcgatc ccagcctcgc ccactgtgcc gactacctga gcgcctgagt
     3961 cgtcaaccac cttcttgccc gcctcgtgag cgacaactcg ttgacctgcg gtaaacgcgt
     4021 ggtgttgtcg ctaattgctc actcctcgtt atgccgtttg acctcgggaa acaccgctgt
     4081 cgctacgagg cgggcaaagg cactcatgct cccgcggcgc acgaacgtca gcccacggcg
     4141 cggcccgcgc ccttccagaa cttcgcgcgc accgccttct tgtccggctt gcccagcccg
     4201 gtcagcggca gcgcgtcgac gaccacgacc tgcttcggcg actgcactga ccctttgcgc
     4261 tccttgacgg cagcctggat ctcgacggtc atcgccgtca cagattcctg gtcgcgcggc
     4321 gcatcctcgc gcagcaccac gaccgcggtg acggcctc
//
LOCUS       DASWBF010000567         7295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_459082,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000567 DASWBF010000000
VERSION     DASWBF010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459082"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..110)
                     /locus_tag="VF874_21245"
     tRNA            complement(38..110)
                     /locus_tag="VF874_21245"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Ala,seq:tgc)
     gene            complement(224..297)
                     /locus_tag="VF874_21250"
     tRNA            complement(224..297)
                     /locus_tag="VF874_21250"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Ile,seq:gat)
     gene            complement(355..1197)
                     /locus_tag="VF874_21255"
     CDS             complement(355..1197)
                     /locus_tag="VF874_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEX9178495.1"
                     /translation="MSSPNEPGNGNAPAPGAAQSTGSGPVSTTPETGTGDVPPWERAA
                     AARPQQPPPAPPRRAETSGPHGASGIEERINRFISGSSASTQTPEPEAPAPPRPDAPA
                     RSETIRSELPDLSGPAPRPSRAKPPAERPSGEPPAPTPRSGAGAARSAATGRVQVGTR
                     TSGPVRASMQIRRVDPWSVLKVSLVLSVALFFVWMIAVAFLYLVLGGMGVWAKLNSNV
                     GDLLNNTTNSELVSSGTIFGGAALIGLMNIVLLTAMATVGAFVYNLTTDLVGGVEVTL
                     ADRD"
     gene            complement(1208..4282)
                     /gene="gyrA"
                     /locus_tag="VF874_21260"
     CDS             complement(1208..4282)
                     /gene="gyrA"
                     /locus_tag="VF874_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intein-containing DNA gyrase subunit A"
                     /protein_id="HEX9178496.1"
                     /translation="MTDTTLPPDDAVDRVEPVDIQQEMQRSYIDYAMSVIVGRALPEV
                     RDGLKPVHRRVLYAMFDSGFRPDRSHAKSARSVAETMGNYHPHGDASIYDTLVRMAQP
                     WSLRYPLVDGQGNFGSPGNDPPAAMRYCVTGDALVSLPDGKSIRIRDVVPGAQPNSDN
                     VIDVKVLGRQGTPVVADRLFHSGEHQTYTVRTVEGYEVTGTANHPLLCLVDVAGVPTL
                     LWKLVEEIRPGDYAVMLAETTVVDENQPKSVHNRSVGEVPGLAEFVATGDPDAQAIAA
                     ELTDGRYHYARVGSLTDASVQPVYSLRVDTADHAFVTNGFVSHNTEARLTPLAMEMLR
                     EIDEETVDFIPNYDGRVQEPTVLPSRFPNLLANGSGGIAVGMATNIPPHNLRELAEAV
                     FWCLENHEADEEKTLKAVMKRVKGPDFPTAGLIVGSQGITDAYTTGRGSIRMRGVAEI
                     EEDSRGRTSLVITELPYQVNHDNFITSIADQVRDGKLNGIANIEDQGSDRVGIRIVIE
                     LKRDAVAKVVLNNLYKHSQLQTSFGTNMLAIVDGVPRTLRLDQMIRLYVEHQLDVIIR
                     RTRYRLRKANERAHILRGLVKALDALDEVIALIRASQTVDDARQGLIELLDIDEIQAQ
                     AILDMQLRRLAALERQRIVDDLAKIEAEIADLEDILAKPERQRTIVRDELKEIADKHG
                     DDRRTRIIAADGEVSDEDLIAREDVVVTITETGYAKRTKTDLYRSQKRGGKGVQGAGL
                     KADDIVNHFFVCSTHDWILFFTTQGRVYRAKAYDLPEASRTARGQHVANLLAFQPDER
                     IAQVIQIRSYEDAPYLVLATRNGLVKKSKLTDFDSNRSGGIVAINLRDSDELVGAVLC
                     SGEDDLLLVSAKGQSIRFTASDEALRPMGRATSGVQGMRFNTDDALLSLNVVVPDTYL
                     LVATSGGYAKRTAMDEYPVQGRGGKGVLTIQYDRRRGTLVGALVVDDDTELYAITSGG
                     GVIRTAARQVRKAGRQTKGVRLMNLGEGDTLVAIARNAEEGDSADAEPDSTT"
     gene            complement(4312..6339)
                     /gene="gyrB"
                     /locus_tag="VF874_21265"
     CDS             complement(4312..6339)
                     /gene="gyrB"
                     /locus_tag="VF874_21265"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX9178497.1"
                     /translation="MAAQRKKAQTEYGAQSITILEGLEAVRKRPSMYIGSTGERGLHH
                     LIWEVVDNAVDEAMAGYAAKVDVRLLEDGGVEVIDDGRGIPVATHASGVPTVDVVMTQ
                     LHAGGKFDSDAYAVSGGLHGVGVSVVNALSSRLEVEISRDGYEWFQQYDHSVPGTLKQ
                     GEKTKKAGTTVRFWADPNIFETTEYDFETVARRLQEMAFLNKGLTIELTDERVSQEEV
                     VDEVVSDTAEAPKTAEEKAAEAIAPHKVKHRMFHYPGGLVDFVKHINRTKQAIHSSVV
                     DFQGKGPGHEVEVAMQWNAGYSESVHTFANTINTHEGGTHEEGFRAALTSVVNKYAKD
                     RKLLKDKDPNLTGDDIREGLAAVISAKVAEPQFEGQTKTKLGNTEVKSFVQRVCNEQL
                     THWFESNPADAKVVVNKAVSSAQARIAARKARELVRRKSATDIGGLPGKLADCRSTDP
                     RKSELYVVEGDSAGGSAKSGRDSMFQAILPLRGKIINVEKARIDRVLKNTEVQAIITA
                     LGTGIHDEFDLAKLRYHKIVLMADADVDGQHISTLLLTLLFRFMRPLIENGHVFLAQP
                     PLYKLKWQRSEPEFAYSDRERDGLLEAGMKAGKKINKEDGIQRYKGLGEMDAKELWET
                     TMDPSVRVLRQITLDDAAAADELFSILMGEDVEARRSFITRNAKDVRFLDV"
     gene            complement(6563..7087)
                     /locus_tag="VF874_21270"
     CDS             complement(6563..7087)
                     /locus_tag="VF874_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 family protein"
                     /protein_id="HEX9178498.1"
                     /translation="MTDDGRDLVRRVLEEARSAARARGKNIGQGRQPARTPRVAGRKG
                     GGRRAWSGPGPDARDPQLLGTVTRDLAATRGWSARVAEGTVFGSWASVVGAQISDHAS
                     PTALHDGVLNVTAESTAWATQLRMMQAQLLAKIAAAVGDGVVTALKITGPAAPSWRKG
                     PLRVAGRGPRDTYG"
     gene            complement(7084..>7295)
                     /locus_tag="VF874_21275"
     CDS             complement(7084..>7295)
                     /locus_tag="VF874_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX9178499.1"
                     /translation="GTDPVLLLDDVFAELDTARRRSLAVVAGEAEQVLLTAAVPDDIP
                     LDLDARRITIGLAEDDGGRVSVVTA"
BASE COUNT         1094 a         2430 c         2387 g         1384 t
ORIGIN      
        1 gggaaatccg ccgccggcag gcggtcgcac atcgttgtgg agcctagggg attcgaaccc
       61 ctgacttctg ccttgcaaag gcagcgctct accaactgag ctaaggcccc ttgggttcgt
      121 cggctgcgtg ccagacctcg ccggcgcgcc gtgaccgcac gatcagcacc gccaccgcga
      181 gcacggcgag cagtacgacg agcctcatgg tgcacctctt ccgtggggct aggaggactc
      241 gaacctccga cctcttcgtt atcagcgaag cgctctaacc gcctgagcta tagccccgaa
      301 catgcgtgga ccgagcgacg agattaccgc aatcgcggcc acccgcccaa accctcaatc
      361 ccgatcggcg agcgtcacct cgacgccgcc gacgagatcg gtagtgaggt tgtaaacgaa
      421 cgcgccgacg gtggccatcg cggtcagcag cacgatgttc atcaggccga tcaacgctgc
      481 accgccgaag atcgtgccgc tagacaccaa ctcgctgttg gtggtgttgt tcagcaagtc
      541 gcccacgttg ctgttgagct tggcccagac ccccatcccg ccgagcacca ggtacaggaa
      601 cgcgaccgcg atcatccaga cgaaaaacag cgccaccgac agcacgagcg acaccttcag
      661 cacgctccac gggtcgacgc ggcggatctg catgctggcg cgcacggggc cactggtgcg
      721 agtgcccacc tgcaccctgc ctgttgccgc cgaccttgcg gctcccgcgc ccgaccgcgg
      781 ggtcggagca ggcggttcgc cactcggtcg ctcggccggc ggtttggccc gcgaaggacg
      841 tggggccgga ccggacaggt cgggcagctc actgcggatg gtttcgctgc gggcgggcgc
      901 atcgggccgc ggaggtgccg gcgcctcggg ctcgggtgtc tgcgtgctgg ccgacgaccc
      961 ggagatgaag cggttgatcc tctcctcgat accggatgcc ccgtgcggcc cggaggtctc
     1021 cgcgcgccgc ggtggcgccg gcggcggctg ctgaggccgg gcagctgcgg cgcgctccca
     1081 gggcggcacg tcgcctgtcc cggtttccgg cgtcgtcgag accggacctg atcccgtcga
     1141 ttgggcggcg cccggtgcgg gcgcgttgcc gttgcccggc tcgttcggtg agctcaccta
     1201 cggctcctta cgtggtcgag tccggttcgg cgtccgcact gtcaccttcc tcggcgttgc
     1261 gtgcaatggc aaccagtgtg tcgccctcgc ccaggttcat caatcgaaca cccttggtct
     1321 ggcgtccggc cttgcggacc tgtcgcgcgg cggtgcggat cacgcctccg cccgaggtga
     1381 tcgcgtacaa ctcggtgtcg tcgtcgacca ccagcgctcc caccagggtg ccacggcggc
     1441 ggtcgtattg aatggtcagc acccctttac cgccgcggcc ctgcaccggg tactcgtcca
     1501 tggccgtgcg cttggcgtag ccgccggaag tcgcgaccag cagatacgtg tccggaacga
     1561 ccacgttcag ggacagcagc gcgtcgtcgg tgttgaaccg catgccctgc acgccggacg
     1621 tcgcgcggcc catcggacgc agcgcctcgt cgctggcggt gaaccggatc gactggccct
     1681 tcgccgacac cagcagcaga tcgtcctctc cggagcacag cactgcgccg acgagctcgt
     1741 cgctgtcgcg caggttgatc gcgacgatgc cgccggagcg gttggagtcg aagtcggtga
     1801 gcttggattt cttcaccagc ccattgcggg tggccaacac caggtacggg gcgtcctcgt
     1861 agctgcggat ctggatcact tgcgcgatgc gttcgtccgg ctggaaggcc agcaggttcg
     1921 cgacgtgctg gccgcgcgcc gtgcgcgacg cctccggaag gtcgtacgcc ttggcgcgat
     1981 acacccgccc ctgggtggtg aagaacagga tccagtcgtg cgtggagcag acgaagaagt
     2041 ggttgacgat gtcgtcggcc ttgagcccgg cgccctgcac gcccttgccg ccgcgctttt
     2101 ggctgcggta caggtcggtc ttggtgcgct tggcgtaacc ggtttcggtg atcgtgacga
     2161 cgacgtcttc gcgggcgatc aggtcctcgt cggacacttc gccgtcggcg gcgatgatgc
     2221 gcgtgcgccg gtcgtcgccg tgcttgtcgg cgatctcttt gagttcgtcg cgcacgatgg
     2281 tgcgctggcg ttccggcttg gcgagaatgt cttccaggtc ggcgatttcg gcttcgatct
     2341 tggcgagatc gtcgacgatg cgctgacgtt cgagtgctgc gaggcggcgc agctgcatgt
     2401 cgaggatggc ttgagcctgg atctcgtcga tgtcgagcag ctcgatcagg ccctggcgcg
     2461 catcgtccac ggtttgtgac gcgcggatca gcgcgatcac ctcgtcgagc gcgtcgagtg
     2521 ccttgaccag gccgcgcaga atgtgggcgc gctcgttggc ttttcggagg cggtagcggg
     2581 tgcgccggat gatgacgtcg agctggtgct cgacgtacag ccggatcatc tggtcaagcc
     2641 ttagggtgcg cgggaccccg tcgacgatcg cgagcatgtt ggtgccgaag ctggtctgca
     2701 gctgcgagtg cttgtagagg ttgttcagca caaccttggc gaccgcgtcg cgcttgagtt
     2761 cgatgacgat gcggatgccg acccggtcgc tgccctgatc ctcgatgttg gcgattccgt
     2821 tgagctttcc gtctcggacc tggtcggcaa tcgaggtgat gaagttgtcg tggttgacct
     2881 ggtaggggag ttcggtgatg accagcgagg tgcggccgcg gctgtcttcc tcgatctcgg
     2941 cgacgccgcg catccggatc gagccgcggc cggtggtgta ggcatcggta atcccttgcg
     3001 agccaacgat caagcccgcg gtggggaagt cgggaccctt gacccgcttc atcacggcct
     3061 tgagggtctt ctcctcgtcg gcctcgtggt tctccaggca ccagaacacc gcttcggcca
     3121 gctcgcgcaa attgtgcggc gggatgttgg tggccatgcc gaccgcgatg ccgccggaac
     3181 cgttggcgag caggttcgga aaccggctcg gcagaaccgt cggttcctgc acccggccgt
     3241 cgtagttcgg gatgaaatcg actgtctctt cgtcgatttc gcgaagcatc tccatggcca
     3301 gcggagtgag ccgcgcttcg gtgttgtggc tgacgaagcc gttggtgacg aaggcgtggt
     3361 cggctgtgtc gacacgtaag ctgtagaccg gctgcacgct ggcgtcggtc aggctgccta
     3421 cgcgcgcgta gtggtagcgg ccgtcggtga gctcggcggc aatagcctgt gcgtcgggat
     3481 ctccggtcgc gacaaactcc gccaagcccg ggacttcgcc gacactacgg ttgtgtacgg
     3541 atttcggctg attttcgtca acaaccgtag tctcggcgag cattacggcg taatcgccag
     3601 ggcgaatctc ctcgacgagt ttccacagca gtgtcggcac gccggccacg tcgaccaggc
     3661 acagcagcgg gtggttcgcc gtgccggtga cttcataccc ctcgaccgtc cgcaccgtgt
     3721 acgtctggtg ctcgccggaa tggaacagcc gatcggcgac gacgggagtt ccttgccggc
     3781 caagcacttt cacgtcgatg acgttatcgg agttgggctg cgcgccgggc accacatcgc
     3841 gaatccggat cgacttgcca tccggcaggc tcaccagtgc atcgcccgtg acgcagtacc
     3901 tcatcgcggc cggcggatcg ttgcccggcg agccgaagtt gccctggccg tcgaccagcg
     3961 ggtagcgcag cgaccacggc tgcgccatcc gcaccaacgt gtcgtagatc gacgcgtcgc
     4021 cgtgcggatg atagttgccc atcgtttcgg ccacggagcg agcggatttc gcgtggctgc
     4081 ggtccggccg gaagcccgag tcgaacatcg cgtacagcac ccgccggtgc actggcttga
     4141 ggccgtcgcg cacctcaggc agcgcgcggc ccacgatcac gctcatcgcg taatcgatgt
     4201 agctgcgctg catctcctgc tggatgtcga ccggctcgac gcggtcgacc gcgtcgtcag
     4261 gtggcagcgt tgtatcagtc atgtaagccc cgtcagttgg ttaaacttcg cctaaacatc
     4321 aaggaagcgg acgtctttcg cgttgcgggt gatgaagctg cgacgcgcct cgacgtcctc
     4381 gcccatcagg atcgagaaca gctcgtcagc cgcggcggcg tcatccagcg tgatctggcg
     4441 gagcacgcgc accgatgggt ccatcgtggt ctcccagagt tccttggcgt ccatctcgcc
     4501 gagacccttg taccgctgga tgccgtcctc cttgttgatc ttcttgccgg ccttcatccc
     4561 tgcttccaac agcccgtcgc gctcgcggtc ggagtacgca aattccggct cggaccgctg
     4621 ccatttcagc ttgtacagcg gcggctgcgc gaggaacacg tgcccgtttt cgatcagcgg
     4681 ccgcatgaac cggaacagca acgtcaacag cagcgtcgaa atgtgctggc cgtcgacatc
     4741 ggcgtcggcc atcaaaacga tcttgtggta gcgcagtttg gcgaggtcga actcgtcgtg
     4801 gatgccggtg cccagcgcgg tgatgatcgc ctgcacctcg gtgttcttca gcacccggtc
     4861 gatgcgcgct ttctcgacgt tgatgatctt gccgcgcagc ggcaggatgg cctggaacat
     4921 cgaatcgcgg ccgctcttcg ccgagccgcc ggccgaatcg ccttccacca catacagttc
     4981 cgacttgcgg ggatcggtgg agcggcagtc ggcgagctta ccgggcaggc cgccgatgtc
     5041 ggtggcgctc ttgcgccgca ccagctcccg cgccttgcgc gccgcgatgc gcgcctgcgc
     5101 cgacgagacc gccttgttca ctaccacttt cgcgtcggcg gggttggact cgaaccagtg
     5161 cgtcaactgc tcgttgcaca cccgctgcac gaacgacttc acctcggtgt tgcccagctt
     5221 ggtcttggtc tggccctcga actgcggctc ggccaccttg gccgagatga ccgccgccag
     5281 cccctcccgg atgtcgtcgc cggtgaggtt ggggtctttg tccttcagca gctttcggtc
     5341 cttggcgtac ttgttcacca ccgaggtcag cgccgcgcga aaaccctcct cgtgggtacc
     5401 gccctcatgg gtgttgatgg tgttcgcgaa ggtgtgcacc gattccgaat agccggcgtt
     5461 ccactgcatc gccacctcga cctcatggcc ggggcccttg ccctggaagt cgacgacgct
     5521 gctgtggatc gcctgcttcg tccggttgat gtgcttgacg aaatccacca atccgccggg
     5581 gtagtggaac atccggtgct tgaccttgtg cggcgcgatc gcttcggcgg ccttctcctc
     5641 cgccgttttc ggcgcctcgg cggtgtcgct gacgacctcg tcgaccacct cttcctgcga
     5701 cacccgctcg tcggtgagct cgatggtcag gcccttgttc aggaacgcca tctcctgcag
     5761 tcggcgtgcg acggtttcga agtcgtattc ggtggtttcg aagatgttcg ggtcggccca
     5821 gaaccgcacc gtggtgccgg ccttcttggt tttctcgccc tgcttgagcg tgccgggcac
     5881 ggagtggtcg tactgctgaa accactcgta gccgtcgcgc gaaatttcga cctcgagccg
     5941 actggacagc gcgttgacca ccgagacacc gacaccgtgc aggccaccgg acaccgcgta
     6001 ggcgtcggaa tcgaacttgc ccccggcgtg cagttgcgtc atcacgacgt cgacggtcgg
     6061 aacgccggac gcgtgggttg cgaccgggat gccgcggccg tcgtcgatca cttcgacccc
     6121 gccgtcctcg agcagccgca cgtcgacctt ggcggcgtag cccgccatcg cttcgtcgac
     6181 cgcgttgtcc acgacctccc agatcaggtg gtgcaggccg cgctcgccgg tggagccgat
     6241 gtacatgctc gggcgtttgc ggacagcctc cagcccctcg agaattgtga tggattgggc
     6301 gccgtactcc gtttgagctt tctttctctg ggcagccaca gtcggacagt tctccttggg
     6361 gttcgcatgc gagcggttcg gtcaccgcga gcggggtctg cgtccagtct accgtcaacc
     6421 gccgtcagca ccgattctcc ggcggcgttt ctacacacct aataacgccg tgcacgaaat
     6481 ttctcgacca cggcctcgtc ctgacgcttg cgaacattat ttgcgacgtt cagacggctc
     6541 tcagaccact aagatgggcc ggtcatccgt acgtgtcccg agggccgcgg ccggccaccc
     6601 gaagcggccc cttccgccac gacggcgcgg ccggaccggt gatcttgagc gccgtcacga
     6661 caccgtcccc gaccgccgcg gcaatcttgg ccagcagttg cgcctgcatc atccgcaact
     6721 gagtggccca ggcggttgac tccgccgtca cgttcagcac cccgtcgtgc agggcggtgg
     6781 gcgaggcgtg gtcggagatc tgcgcgccga ccaccgacgc ccaggagccg aacacggtgc
     6841 cttctgcgac ccgtgccgac cagccgcggg tcgcggcgag atccctggtg acggtgccga
     6901 gaagttgcgg atcgcgggcg tcgggaccag gcccagacca ggcgcgacgg ccgccgccct
     6961 tccgtccggc cacacgcgga gtccgagccg gctggcggcc ctgtccgatg ttcttacccc
     7021 gcgcccgtgc ggcgctgcgg gcctcctcca gcacgcggcg gacgagatcc ctcccgtcgt
     7081 cggtcatgcc gtcaccaccg acacccggcc accgtcgtct tcggccaacc cgatcgtgat
     7141 gcgtcgggcg tcgagatcga ggggaatgtc gtcggggact gccgccgtca gcaggacctg
     7201 ctctgcctcg cctgcgacca ccgccaacga ccggcggcgc gcggtgtcga gttccgcgaa
     7261 cacgtcgtcg agcagtagca caggatcggt gccct
//
LOCUS       DASWBF010000568         6800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_124318,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000568 DASWBF010000000
VERSION     DASWBF010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124318"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF874_21280"
     CDS             complement(<1..228)
                     /locus_tag="VF874_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178500.1"
                     /translation="MNQASYDLARLRINGLITRVPGRNRYRLTDDGLRFAIFYTKLHD
                     RLLRTLLAADRPPAPPALRKALHTIDTHITEP"
     gene            325..567
                     /locus_tag="VF874_21285"
     CDS             325..567
                     /locus_tag="VF874_21285"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9178501.1"
                     /translation="MRGGVGTVLPGRTGRRDQALLALAVQTGLRISELITLTRNDIHT
                     ATGAHITCLGKGRRHRATPLTRHHAHRHGALPDRTG"
     gene            539..835
                     /locus_tag="VF874_21290"
     CDS             539..835
                     /locus_tag="VF874_21290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9178502.1"
                     /translation="MEPYLTERADRPGDALFPRPDGAHLSRDALEHRLAKHLNTAAAT
                     CPSLRGKHVTMHTLRHTAAMRLLQASVDVSVIALWLGHQHTSTPAAPTSTSTPT"
     gene            1019..1195
                     /locus_tag="VF874_21295"
                     /pseudo
     CDS             1019..1195
                     /locus_tag="VF874_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
     gene            complement(1279..1545)
                     /locus_tag="VF874_21300"
     CDS             complement(1279..1545)
                     /locus_tag="VF874_21300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9178503.1"
                     /translation="MREITASEASRNFSAVLDSVENGEKVVVTRAGRRIAAISPGPAA
                     TGAALNAVLRRWRGIDTIDDAFSENIAAARAAVSTDNDTDPRTG"
     gene            complement(1583..1936)
                     /locus_tag="VF874_21305"
     CDS             complement(1583..1936)
                     /locus_tag="VF874_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX9178504.1"
                     /translation="MSETAAQFPDEELLADVEEAMRDVVDPELGINVVDLGLVYGLNV
                     ERGEEGTVALIDMTLTSAACPLTDVIEDQSRMALVGAGLVNDIKINWVWNPPWGPDKI
                     TEDGRDQLRALGFTV"
     gene            complement(1905..2399)
                     /locus_tag="VF874_21310"
     CDS             complement(1905..2399)
                     /locus_tag="VF874_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX9178505.1"
                     /translation="MRLEQLYQEVILDHYKHPHHRGLREPYGAEVFHVNPICGDEVKL
                     RVALSDDGERVTDVSYDGQGCSISQASTSVLTDQVIGQTVGDALKTVAAFTEMVSSRG
                     TIEGDEDVIGDGIAFAGVAKYPARVKCALLGWMAFKDALAQASAGTHVQEVDDERNGR
                     SIPG"
     gene            complement(2401..3654)
                     /locus_tag="VF874_21315"
     CDS             complement(2401..3654)
                     /locus_tag="VF874_21315"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX9178506.1"
                     /translation="MTASVTSLDITAIRADFPILKRVMRGGSQLAYLDSGATSQRPLL
                     VLDAERDFLTTSNGAVHRGAHQLMEESTDAYEQGRGDIAAFVGADTDELVFTKNATEA
                     LNLVSYVLGDSRFSRAVGSGDVIVTTEIEHHANLVPWQELARRTGATLRWYGVTDDGR
                     INLDSLELDERVKVVTFSHHSNVTGALSPVAELVSRARAVGALTVLDACQSVPHQPVD
                     LHALGVDYAAFSGHKMLGPNGIGVLYGRRELLNEMPPFITGGSMIETVTMEATTYAPP
                     PQRFEAGTPMTSQVVGLAAAARYLGTIGMDRVEAHERQLVAAALDGLSHVKGVRIVGP
                     TSMTDRGSPVSFVVAGIHAHDVGQVLDDDGVAVRVGHHCAIPLHRRFGIAATARASFA
                     LYNTFDEVDRLVAGVRRAVDFFGGA"
     gene            complement(3657..4427)
                     /gene="sufC"
                     /locus_tag="VF874_21320"
     CDS             complement(3657..4427)
                     /gene="sufC"
                     /locus_tag="VF874_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX9178507.1"
                     /translation="MTTLEINDLHVSVTPSEEEEIPILKGVDLTVKSGETHAMMGPNG
                     SGKSTLGYAIAGHPKYKVTSGSITLDGEDVLAMTIDERARAGLFLAMQYPFEVPGVSM
                     SNFLRTAATAVRGEAPKLRHWVKEVESAMDDLDIDPVFAERSVNEGFSGGEKKRHEIL
                     QLALLKPKIAILDETDSGLDVDALRVVSEGVNRYAEETRGGILLITHYTRILRYIQPQ
                     FVHVFVGGRFIESGGPELADELDANGYERFSQAAVAEA"
     gene            complement(4424..5602)
                     /gene="sufD"
                     /locus_tag="VF874_21325"
     CDS             complement(4424..5602)
                     /gene="sufD"
                     /locus_tag="VF874_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX9178508.1"
                     /translation="MVQDLTATLEGRTKGEQFTSFDVNAFEVPGGRDEIWRFTPLRRL
                     RGLHNGSAPATGTADIAVSAPPGVTVETVRRGDDRLGRAGVPADRVAAQAFSSFNRAT
                     IVTVERAATPGDAIDIAITGPGEGATAYGHLQLRVGELAEAVVVIDQRGSGTYADNVE
                     FVVGDAARLTVVSIADWANDAVHVSTHHAALGKDAVLRHVVVALGGDLVRLTARVRFA
                     APGGDAELLGLYFADDGQHLESRLLIDHAQPNCRSHVTYKGALQGDPDSSRPDAHTVW
                     VGDVLIRAQATGTDTFETNRNLVLTDGARADSMPNLEIETGEIIRAGHASATGRFDDE
                     QVFYLRSRGIPEDQARRLIVRGFFNEIIAKISLPAVRERLTDAIEEEFALTESIQGSK
                     "
     gene            complement(5602..>6800)
                     /gene="sufB"
                     /locus_tag="VF874_21330"
     CDS             complement(5602..>6800)
                     /gene="sufB"
                     /locus_tag="VF874_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX9178509.1"
                     /translation="VYTKAGYAAMRDNVGIGSSSKKLSDLLMRQDETFLRELVDAYIN
                     GDGNVTRRGGALWKRAHTTSRVWAFQLQAILARLGHYATVELRRPGGPGVILGRNIVR
                     KDIYQVQWTEGGTGPRQARDCGDYFTVPIKKRAVREAHEPVFNLDVEEPDSYLAYGFA
                     VHNCTAPIYKSDSLHSAVVEIIVKPGGRCRYTTIQNWSNNVYNLVTKRARAEAGATME
                     WIDGNIGSKVTMKYPAVWMTGEHAKGEVLSVAFAGEGQHQDTGAKMLHLAPNTSSTIV
                     SKSVARGGGRTSYRGLVQVNKGAHGSRSSVKCDALLVDTISRSDTYPYVDIREDDVTM
                     GHEATVSKVSADQLFYLMSRGLTEDEAMAMVVRGFVEPIAKELPMEYALELNRLIELQ
                     MEGAVG"
BASE COUNT         1139 a         2354 c         2118 g         1189 t
ORIGIN      
        1 tggttcggtg atgtgagtgt cgatggtgtg caaggccttt cgcagtgcag gtggcgcggg
       61 cggcctgtcg gcagccagca gcgtgcggag cagccggtcg tggagtttgg tgtagaagat
      121 cgcaaaccgc agtccgtcgt cggtgagccg gtagcggttt ctgccgggga cgcgggtgat
      181 gagcccgttg atgcgcagcc gcgccaggtc gtagctggcc tggttcatgg tgtaggtcgc
      241 accgccgagc aggccggcag gtgctggtgt ccggcgccgc cagcagcgac tcggtctcgc
      301 cgatggtgag gaactcgacg gtaggtgcgg ggcggcgtcg ggacggtatt gccaggacgg
      361 accggccgcc gcgaccaagc gttgctggcc ctggctgttc agacgggact gcggatcagt
      421 gaactgatca ccttgacccg caacgacatt cacaccgcaa ccggtgcgca catcacctgc
      481 ctcggcaaag gacgccggca ccgcgccaca cccctgaccc gccaccacgc tcaccgtcat
      541 ggagccctac ctgaccgaac gggctgatcg ccccggagac gcactgttcc ctcgccccga
      601 cggagctcac ctatcccgcg acgccctcga acaccgcctc gccaaacacc tcaacaccgc
      661 cgcggcgacc tgcccatcac tgcggggcaa acacgtcacg atgcacacgc tgcgccacac
      721 cgccgcgatg cgactgctcc aagccagcgt cgatgtctct gtgatcgcgc tctggctcgg
      781 ccaccagcac accagcacac cagcagcacc gacgtctacc tccacgccga catgacgatc
      841 aagcaagcag caattgaccg gacccgcccg cctcacatcc ccgctggcac ctacacccca
      901 cccccggaca tcctgaccta gctcgacagc ctctgactat gtcgacacca caaccgatcc
      961 cacgctgcag accagcaccg ataccccgat catcggcata atccaaacct cggcatagcc
     1021 ggtctaccac cacacccggg agtcgatcga ggcgcacttg acgatcgtgt tcgccgcact
     1081 ggccgtcagt cactggatcg aacaccaaac cggctggagc atcaagaaat tcgtgcgcac
     1141 cgcacgccgc taccgcaccg tccaaatcaa ggccggccga cagaccctga ccgccgagcg
     1201 cgggtatggc gacatccgct ttctccggca ccatcgaaga cccgcgcacc gcagcgacca
     1261 gtacgccggt gtcgaggatc aaccggtccg cgggtcggtg tcgttgtccg tcgatacggc
     1321 agcgcgagcg gcggcgatgt tctcactaaa ggcgtcatcg atcgtgtcta ttccccgcca
     1381 acgccgaagc actgcgttca acgccgcacc agttgcagca gggcctggac taattgcggc
     1441 aattcgacgc ccagcacgag tcacaacaac cttctcgccg ttctcgaccg aatcgagcac
     1501 ggcggagaag tttcgtgacg cctccgaggc cgtgatctcg cgcatggatt cggatcatca
     1561 gattctgaag atctgagcat cgctaaaccg taaaccccaa cgcccgcagt tggtctcggc
     1621 cgtcctcggt gatcttgtcc gggccccacg gcggattcca cacccagttg atcttgatgt
     1681 cgttcaccag cccggctccc accagcgcca tccgcgactg gtcctcgatc acatcggtca
     1741 gcgggcaggc cgccgacgtc agcgtcatgt cgatcagcgc gaccgtgccc tcctcgccgc
     1801 gctctacgtt caacccgtag accaagccga ggtcgacgac gttgatgcca agttcggggt
     1861 cgacgacgtc gcgcatcgcc tcctcgacgt cggcaagcag ctcctcatcc gggaattgag
     1921 cggccgtttc gctcatcgtc cacctcctgc acatgcgtcc cggcgctagc ctgtgccagc
     1981 gcgtctttga aagccatcca tcccagcagc gcgcatttca cgcgcgccgg gtacttcgcc
     2041 accccggcga acgcgatgcc gtcgccgatg acgtcttcgt ctccttcgat tgtgccgcgc
     2101 gacgagacca tctcggtgaa cgcggcgacc gtcttgagcg catcaccgac ggtctggccg
     2161 atcacctggt cggtgagcac cgacgttgac gcctggctga tcgagcagcc ctggccgtca
     2221 tacgaaacgt cggtcacccg ctccccgtcg tcactgagcg caacccgtag cttcacctcg
     2281 tcaccgcata tcgggttcac gtggaagacc tcggcgccgt acggctcgcg cagcccgcga
     2341 tggtgcgggt gcttgtagtg atcgaggatc acttcctgat agagctgctc cagccgcaac
     2401 ttatgccccg ccgaagaaat cgaccgcccg acgcacgccg gccaccagcc ggtctacttc
     2461 gtcgaacgtg ttgtagagcg cgaacgacgc tcgcgccgtt gcggcgatcc cgaaccgacg
     2521 gtgcagtgga attgcacagt gatgcccgac gcgcaccgcc acaccgtcgt cgtcgagcac
     2581 ctggcccacg tcgtgcgcgt gaatgccggc gacgacaaat gacaccggag aaccgcggtc
     2641 ggtcatcgac gtcgggccga caatccgaac acctttgacg tgtgacaacc cgtccagcgc
     2701 ggccgccacc agctgacgct cgtgcgcctc gaccctatcc ataccgatgg tccccagata
     2761 ccttgccgca gcggctaatc cgacgacctg cgacgtcatc ggcgtgccgg cctcgaaccg
     2821 ctgcggcggc ggcgcgtagg tggtggcctc catcgtgacg gtttcgatca tggacccgcc
     2881 ggtgatgaac ggcggcatct cgttcagcag ctcgcgacga ccgtacagca caccgatccc
     2941 gttggggccc agcatcttat ggccggagaa cgccgcgtaa tccacgccga gcgcatggag
     3001 gtccaccggc tggtgcggca ccgactggca ggcgtcgagc accgtcagcg cgccgaccgc
     3061 ccgtgcccgc gaaaccagct cggccacggg cgacagcgcg ccggtgacat tcgaatggtg
     3121 gctgaacgtg acgactttca cccgctcgtc gagctcaagc gaatcgaggt tgatgcggcc
     3181 gtcgtcggtg acgccgtacc agcgcagggt cgcgccggtc cggcgcgcca actcctgcca
     3241 cgggaccaga ttcgcgtggt gttcgatctc ggtggtgact atcacgtcac cagatccgac
     3301 ggcacggctg aaccggctgt cgccgagcac ataggagacg agattcaatg cctcggtggc
     3361 gttcttggtg aacaccaact cgtctgtgtc ggcgccgacg aacgccgcga tgtcgccgcg
     3421 gccctgctcg tacgcgtcgg tggactcctc catcagttga tgtgcgccgc ggtgcaccgc
     3481 accgttcgac gtggtcaaga agtcgcgctc ggcgtcgagt accagtagcg gccgctgcga
     3541 cgtcgcaccg gaatccaggt acgccaactg gcttccgcca cgcatcactc gcttgaggat
     3601 cgggaagtcc gccctgatcg ccgtgatgtc gagtgaagtc accgacgccg tcatcgctat
     3661 gcctccgcga ccgccgcctg ggaaaagcgt tcgtacccgt tcgcgtcgag ttcgtcggcc
     3721 aattccggcc caccggattc gatgaaccga ccgccgacga acacgtgcac gaactgcggc
     3781 tggatgtagc gcaggatgcg ggtgtagtgc gtgatcagca ggatgccgcc gcgggtttcc
     3841 tccgcgtagc ggttgactcc ctcgctgacc acccgcaacg cgtcgacatc cagcccggag
     3901 tcggtctcat cgaggatcgc gatcttgggc ttcaacaagg cgagctgaag gatttcgtgg
     3961 cgcttcttct cgccgcccga aaacccttcg ttgacactgc gctcggcgaa caccgggtcg
     4021 atgtcgaggt cgtccatcgc ggactcgacc tccttgaccc agtgccgcag cttcggcgcc
     4081 tcaccccgca ccgcggtggc cgccgtgcgc aggaagttcg acatcgacac cccgggcacc
     4141 tcgaacgggt actgcatcgc cagaaacagc ccggcgcggg cccgttcgtc gatggtcatc
     4201 gccagcacgt cttcgccgtc cagcgtgatc gagccggagg tgaccttgta cttcgggtga
     4261 ccggcgatcg cgtaccctag cgtcgacttg ccggagccgt tgggccccat catcgcatgg
     4321 gtttcgcccg acttcacggt caagtcgacg cccttgagga tcgggatctc ctcttcctcg
     4381 ctcggcgtga cactgacgtg caggtcgttg atctccagag tcgtcatttg cttccttgga
     4441 tgctttcggt gagcgcgaac tcttcttcaa tggcgtcggt caggcgttcg cgaacggcgg
     4501 gcaaggagat cttggcgatg atttcgttga agaatccgcg cacgatcagc cggcgcgcct
     4561 ggtcttccgg gatgccgcgc gatctcaggt agaacacctg ctcgtcgtcg aaacgtccgg
     4621 tggcgctggc atgcccggcg cgaatgattt cgccggtctc gatctccagg ttcggcatcg
     4681 agtcggcgcg ggcgccgtcg gtcagcacca ggttgcggtt tgtctcgaag gtgtcggtgc
     4741 cggtggcctg cgcccggatc agcacgtcgc cgacccagac agtatgggcg tcgggccgcg
     4801 acgagtccgg atccccttgc agcgcgccct tgtacgtgac atgtgagcga cagttcggtt
     4861 gcgcgtggtc gatgagcagc cgcgactcaa ggtgctggcc gtcgtcggcg aagtacaggc
     4921 cgagcagctc ggcgtccccg ccgggggcgg cgaaccgtac ccgggcggtc agccgcacca
     4981 ggtcgccgcc gagggcgacg accacgtggc gcagcacggc gtccttgccg agcgcggcgt
     5041 ggtgtgtgct cacatgcacc gcgtcgttgg cccagtcggc gatcgagacg acggtcagcc
     5101 gggccgcgtc gccgacgacg aattcgacgt tgtcggcata tgttccgctg ccccgctggt
     5161 cgatgaccac caccgcctcg gcgagttcgc cgacgcgcag ctgcaggtgc ccgtatgccg
     5221 tcgcgccctc ccccgggccg gtgatggcga tatcgatcgc gtccccgggc gtggcggcgc
     5281 gttcgacggt cacgatcgtc gcccggttga acgacgaaaa tgcttgagcg gcaacccggt
     5341 cggcgggcac gccggcccgg ccgaggcggt cgtccccgcg gcgcaccgtc tcgacggtga
     5401 caccgggagg tgcggagacc gcgatgtcgg cggtgccggt ggcgggcgcc gagccgttgt
     5461 gcagcccacg cagcctgcgc aacggggtga accgccagat ctcgtcacgg ccgccgggca
     5521 cctcaaaggc gttgacgtcg aacgacgtga actgctcgcc cttggtcctg ccttccagtg
     5581 ttgcggtcaa gtcctgcacc actagccgac cgcgccttcc atctgcagct cgatgaggcg
     5641 gttgagctcc agcgcgtatt ccatcggaag ttccttggcg atcggctcga cgaagccacg
     5701 gaccaccatc gccatcgcct cgtcctcggt gaggccgcgg ctcatcaggt agaacagctg
     5761 gtccgcgctg accttcgaga cggtcgcctc atggcccatc gtgacgtcgt cctcgcggat
     5821 gtcgacgtag ggataggtat cgctgcggct gatcgtatcg acaagcagcg catcgcattt
     5881 cacgctcgac cgtgacccgt gcgcgccctt gttgacctgg accaggccac ggtaggacgt
     5941 gcggccacca ccgcgggcca ccgacttgga gacgatggtg ctcgacgtgt tgggcgccag
     6001 gtgcagcatc ttggcaccgg tgtcctggtg ctggccctcg ccggcgaacg ccacggacag
     6061 cacctcgccc ttggcgtgct cgccggtcat ccagaccgcc gggtacttca tcgtgacctt
     6121 ggatccgatg ttgccgtcga tccactccat ggtggcgccg gcctccgcgc gggcgcgctt
     6181 ggtcaccagg ttgtagacgt tgttcgacca gttctggatc gtggtgtagc ggcaccgccc
     6241 gcccggcttg acgatgatct cgaccaccgc cgagtgcagc gagtcggact tgtaaatcgg
     6301 cgcggtgcag ttgtgcaccg cgaaaccgta ggccaaatag ctgtccggct cctcgacgtc
     6361 gagattgaag acaggctcgt gcgcttcccg cactgcccgc ttcttgatcg gcacggtgaa
     6421 gtagtcgcca cagtcgcggg cctgcctcgg gccagtgccg ccctctgtcc actgcacctg
     6481 gtagatgtcc ttgcggacga tgttgcggcc caggatgact ccgggcccgc cggggcggcg
     6541 cagttcgacg gtcgcgtagt ggcccagccg cgcgaggatc gcctgtagct ggaacgccca
     6601 gactcgggac gtcgtgtgcg cgcgcttcca cagcgcaccg ccgcgccggg tgacgttgcc
     6661 gtcgccgttg atataggcgt cgacgagctc gcgcaggaac gtctcgtcct ggcgcatcaa
     6721 tagatccgac agctttttgc tcgacgatcc gatgccgacg ttgtcgcgca tcgccgcata
     6781 gcccgccttc gtgtagacgg
//
LOCUS       DASWBF010000569         4676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_850359,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000569 DASWBF010000000
VERSION     DASWBF010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850359"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VF874_21335"
     CDS             <1..457
                     /locus_tag="VF874_21335"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEX9178510.1"
                     /translation="KLQGKSHDIPKKLVWSAWLKVKENGGAAGVDGVTIGQFEERLKD
                     NLYRLWNRMSSGSYFPGPVRAVEIPKKGVTRVLGIPNVVDRVAQTVAVLALEPEVEKV
                     FHDDSYGYRPGRSPLDAIAACRKRCWEKDWVVDLDVKAFLDALSYCGFR"
     gene            complement(466..2685)
                     /locus_tag="VF874_21340"
     CDS             complement(466..2685)
                     /locus_tag="VF874_21340"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9178511.1"
                     /translation="MSESKVTTAHRGRAAVVYVRQSTVAQISRNRESTTRQYDLAARA
                     AELGWPRSAVRVIDDDLGVSGASATGRSGFAELAAQVGLGQVGIVLALEVSRLARNNA
                     DWYRLLDLAGMTDTLIADADGVYHPGLFNDRMLLGMKGTMSEAELHILRARLDGGIRS
                     KAARGELRRGLPVGLVWGEADGEIRWHHDEAVTGVIAAIFERFTVSGSVRGVWLWLRE
                     QQLKFPLQPTAYLRGTDIVWVEPTYHAVHNVLTHPAYAGAYVFGKTRQRRSVDADGAL
                     RVRRHKLPQGEWEVLIKDHHRGFIDWDTFQANQIRISANTRPKAHQPGTGAVREGCAL
                     LQGLATCATCGRKLTVYYDGPAKTTPGYYCTGTGTLVEGKGTRHLRVGGATIDAAVAE
                     AFLAALQPAALQACLAAAQQLEDGHDTALAQWRRQVEHAGYEAGRAERRYRAVDPDNR
                     LVARGLETEWNTALQALADAEAELARRETARPKTLTPQEKSAILALGDDLAEAWSAPT
                     TTDKDRKQLLRSLLEEVNIAVHRDHTDAHAELLLRWKGGAITDLSVPIKRKPINRLRT
                     DEDTVDLVRRLAAHYPDAVIAPILNRQGRRTARGLSFTAGRVQSLRHNYEIPCHTPTD
                     DPKEGEPLTVADAAEQLGLAASTLHRWLADGFITGEQLTPGAPWRIRLTDDIRALFVD
                     DAPDGWLAMLEATLAYGVSRQTIMQRVKRGELKAVHVRTGRRKGLRIQPPPTQEGLF"
     gene            complement(2682..3158)
                     /locus_tag="VF874_21345"
     CDS             complement(2682..3158)
                     /locus_tag="VF874_21345"
                     /inference="COORDINATES: protein motif:HMM:NF036442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5372 family protein"
                     /protein_id="HEX9178512.1"
                     /translation="MRITRPRHPLQGKSLPVLGGMRRHGEPELLLVLPDGSKSLIRAD
                     WTDAAQPGADDVVGVATLGSLTDLLRACKLVADLLGREPEGQGQAAGMSPCKEDSRAA
                     CPAQFDTRPAPGHDSGSAADADGRATAGRRGHDRDHVAGQCDRQSGHTERDRGGRR"
     gene            3212..4507
                     /locus_tag="VF874_21350"
     CDS             3212..4507
                     /locus_tag="VF874_21350"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEX9178513.1"
                     /translation="MDHGIMLKILGEKILDSRFLGLLRNMLRAGYLEDWEWNETLSGA
                     PQGGVVSPILSNIYLDRLDNFVETVLIPEYTRGKLRSHNAEYRQVQYAIAQARKRGDR
                     IEVRALRRKLRGLPTGDPYDPGYRRLRYCRYADDHLLGFTGPKAEAEQIKRRLARFLH
                     EDLKLELSPEKTLITHARTGAARFLGYEITVQHAEHKLVRGRRSVNGVIALRVPMSVI
                     KAKCAPYLKLGKPEHRPERMHLDDHQIVSIYGAEYRGIVQCYLLAGDVWRLNRLHWVM
                     LTSMLKTLAAKHRSTVTKMARKYRTTVVTPHGRRRCFQVSVERNGRKPLVAQFGGIPL
                     RRQKKAILTDRLAAPGATRRKGRELISRVRAGRCELCEQTAEMHVHHVRALADLTQPG
                     RPQPTWAQLMAKRRRKTLVVCRPCHDIIHARPPTATPME"
BASE COUNT          780 a         1467 c         1606 g          823 t
ORIGIN      
        1 gaagttgcag ggcaagtcgc acgacatccc gaaaaagctg gtgtggagtg cgtggttgaa
       61 ggtcaaagag aatggaggag cggccggggt cgatggcgtg acgatcgggc agttcgagga
      121 gaggctgaag gataacctct atcggctgtg gaatcgcatg tcgtcggggt cgtactttcc
      181 cgggccggtc cgcgcggtgg aaataccgaa gaaaggagta acgagggtcc tgggcatccc
      241 gaacgtggtc gatcgggtgg cgcaaacggt ggcggtcctg gcattggagc cggaagtgga
      301 aaaggtcttt cacgacgact cctacggata tcgtcccgga cggtcacccc tggacgcgat
      361 agcggcatgc cgcaaacggt gctgggaaaa ggactgggtc gtcgacctcg acgtgaaggc
      421 cttcttggat gcattgtcat actgcggttt tcgttgattg tcatgtcaga acagtccttc
      481 ctgggtgggt gggggctgga tacgcaggcc ttttcggcgt ccggtgcgca cgtgcacggc
      541 tttgagttcg ccgcgcttga cacgttgcat gatggtctgt cgggacacac cgtaggcgag
      601 ggtggcctcg agcatggcca gccagccgtc gggggcgtcg tcgacaaaca gggcgcggat
      661 gtcgtcggtg agccggatac gccagggtgc gccgggggtg agttgttcgc cggtgatgaa
      721 cccatcggca agccagcggt gcagggtgga ggcggcgagc ccgagctgtt cggcggcgtc
      781 ggctacggtg agtggctctc cttcctttgg atcgtcggtt ggtgtatggc aagggatttc
      841 gtagttgtgg cgcagcgatt ggaccctccc ggcggtgaac gacaatccgc gggcggtgcg
      901 gcggccctgc cggttgagga ttggcgcgat caccgcgtcg gggtagtgag cggccaggcg
      961 gcgcacgagg tcgacggtgt cttcgtcggt gcgcagccga ttgatgggtt tgcgcttgat
     1021 cggcaccgac aggtcggtga tcgcgccgcc tttccagcgc agcagcagtt cggcgtgggc
     1081 gtcggtgtgg tcgcggtgca cggcgatgtt gacctcctcg agcaggctgc gtagcagctg
     1141 tttgcggtcc ttgtccgtgg tggtgggcgc cgaccatgct tcggcgaggt cgtcaccaag
     1201 cgctaggatc gcggatttct cttgtggggt cagggttttc ggtcgggcgg tttcgcggcg
     1261 ggcgagttcg gcctcggcgt cggcgagggc ctgcagcgcg gtgttccatt cggtttccag
     1321 tccgcgggcg accaggcggt tgtccgggtc gacggcgcgg tagcggcgtt cggcgcgacc
     1381 agcctcatag ccggcatgct cgacctggcg gcgccactgc gcgagcgcgg tgtcatggcc
     1441 gtcctcgagc tgctgggcgg cggccaggca ggcctgcagt gcggccggct gcagcgcggc
     1501 caggaacgcc tcggcgaccg cggcatcgat cgtcgctccg ccgacacgca ggtggcgggt
     1561 acccttgccc tcgaccagtg tgccggtgcc ggtgcagtag tagccgggcg tggtcttggc
     1621 cgggccgtcg tagtagacgg tcagtttgcg cccgcaggtg gcgcaggtgg ccagcccctg
     1681 cagcagggcg cagccctcgc ggaccgcccc ggtgcctggc tggtgcgcct tggggcgggt
     1741 gttggcggag atgcggatct gattggcctg gaaggtatcc cagtcgatga atccgcggtg
     1801 atggtctttg atgagcacct cccattcgcc ttgcggcagc ttgtgacggc gcacccgcag
     1861 cgccccgtcg gcatcgacgc tgcggcgctg gcgggtcttg ccgaacacgt aggcgccggc
     1921 gtaggcgggg tgggtgagca cgttgtgcac cgcatggtag gtcggctcga cccagacgat
     1981 gtcggtgccg cgcaggtagg cggtcggttg caacggaaac ttcagctgtt gctcgcgcag
     2041 ccacagccac accccgcgca ccgacccgga cacggtgaac cgctcgaaga tcgcggcgat
     2101 cacgccggtg acggcctcgt cgtgatgcca gcggatctca ccgtcggcct caccccagac
     2161 cagcccgacc ggcagcccgc ggcgcagttc gccgcgggct gccttgctgc ggatcccgcc
     2221 gtcgaggcgg gcgcgcagga tgtgtagttc ggcctcggac atggtgccct tcatgcccag
     2281 cagcatccgg tcgttgaaca agcccggatg gtagacaccg tcggcgtcgg cgatcagcgt
     2341 gtcggtcatc ccggccaggt ccagcagccg gtaccagtca gcgttgttgc gggccagtcg
     2401 tgaaacctca agtgccagaa cgattcccac ctgaccgagg ccgacctggg cggcgagctc
     2461 ggcgaacccg gaacgaccgg tggcgctggc gccggacacc ccgaggtcgt catcgatcac
     2521 ccgcaccgcg ctgcgtggcc aacccaactc ggccgcccgt gcggccaggt cgtattggcg
     2581 agtggtggat tcccggttgc ggctgatctg ggccacggtg gactgccgga catagaccac
     2641 ggcggcacgc ccccgatgcg cggtggtgac ctttgactca ctcatcgccg gccacctcga
     2701 tcccgctcgg tgtggccgct ttggcgatca cattggccag caacatgatc gcggtcgtga
     2761 ccgcgtcggc cggcagttgc tcgaccatcg gcgtcggcgg cggatccgct gtcgtggccg
     2821 ggagcgggtc tggtatcaaa ctgagctgga caggctgcac gggagtcctc cttgcacggt
     2881 gacatccctg cagcctgtcc ctgtccctcg ggctcgcgcc ccagcaggtc ggcgaccagc
     2941 ttgcacgcac gcagcagatc ggtcagcgac ccgagcgtcg ccacgcccac cacatcgtcc
     3001 gcgccgggct gcgccgcgtc ggtccagtcg gcccggatca gcgacttgct gccgtcgggc
     3061 agcaccagca gcaactccgg ctcaccgtgt cgccgcatcc cgcccaacac cggtaacgac
     3121 ttgccctgca acggatgacg cggacgcgtg atccgcaccg tcgtgggcag gtcctcgagg
     3181 cgggtagtaa gtcgtgtgat cgttcgatga gatcgatcac gggatcatgc tcaagatatt
     3241 aggtgaaaag attctcgata gccggtttct ggggcttctg cgtaacatgc tacgagctgg
     3301 atacttggag gattgggaat ggaacgagac gctctccggt gcgccgcagg gcggggtggt
     3361 ttcaccgatc ctgtccaata tttacctgga ccggttggac aattttgtcg aaacagttct
     3421 cataccggaa tacacccgag ggaaactccg tagccacaac gcggagtatc ggcaggtgca
     3481 gtacgcgatc gcgcaggctc gcaaacgcgg tgaccgtatc gaggtgcgcg cgctgcgcag
     3541 gaaactgcgc ggcctaccca ccggggatcc atacgatccc ggctaccgcc ggttgcggta
     3601 ctgcaggtac gcagatgatc atctgctcgg gttcaccgga cccaaagccg aggccgaaca
     3661 gatcaaacgt cgactggcgc ggttcctgca tgaggatctc aaactggaac tgtccccgga
     3721 aaagaccctg atcacgcacg cccgaacggg tgcggcgagg tttctcggct acgagatcac
     3781 cgtccagcac gctgagcaca aactcgtccg cggccgccgg tcggtcaacg gcgtcatcgc
     3841 gctccgcgtg cccatgtcgg tgatcaaggc caaatgcgcc ccctacctca agctcggcaa
     3901 acccgagcat cggcccgaac ggatgcacct cgacgaccac caaatcgtca gcatctacgg
     3961 ggccgaatac cgggggatcg tccaatgcta cctgctggcc ggcgacgtgt ggcgactgaa
     4021 ccggctgcat tgggtcatgt tgacctcgat gcttaagaca ctggccgcca aacaccgctc
     4081 gacggtgacg aagatggccc gcaagtaccg gaccaccgtc gtcacacccc acgggcgtcg
     4141 acggtgcttc caggtcagcg tcgaaagaaa cggcaggaag ccgctggtcg cacagttcgg
     4201 tggcattccg ctacggcggc aaaagaaggc gatcctgacc gaccgcctcg ctgcccccgg
     4261 cgcgacccgc cgtaagggca gggagctcat ctcccgggtt cgggcgggcc gctgtgagct
     4321 ctgcgagcaa acggccgaga tgcacgtgca ccacgtccgc gctctcgccg acctcaccca
     4381 accgggacga ccacagccaa cgtgggctca gctcatggcg aaacggcgac gcaagaccct
     4441 cgtggtctgc cgaccctgcc acgacatcat ccacgcacgg ccaccaaccg ccacgcccat
     4501 ggaataactc actggagagc cgtgatgcgg ggaaacccgc acgtccggtt cgggccggag
     4561 gccgccggaa aaggacctgc tccctgcagg cacctcgccg gcgtcctacc ggtacgtgcg
     4621 tggatgccga tttcggcccg ttctttgtca agttcagtac ctactttttt ccctat
//
LOCUS       DASWBF010000570         4133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_184999,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000570 DASWBF010000000
VERSION     DASWBF010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184999"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..750
                     /locus_tag="VF874_21355"
     CDS             109..750
                     /locus_tag="VF874_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9178514.1"
                     /translation="MQDTQPAPRRVLLVWDAPNLDMGLGSILGGRPTAAYRPRFDALG
                     RWLLARTAELAAGRSDVTLEPEATVFTNIAPGSADVVRPWVEALRNVGFAVFAKPKID
                     DEGDVDADMLNHIALRHSEGLASVLVASADGQAFRQPLEDIARSGVPVQVLGFREHAS
                     WALASDTLDFVDLEDIPGVFREPLPRVGLDSLPEQGAWLQPFRPLSSLLIARV"
     gene            814..3759
                     /locus_tag="VF874_21360"
     CDS             814..3759
                     /locus_tag="VF874_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9178515.1"
                     /translation="MFAWWGRTVYHYRFIVIAVMVGLCLLGGFFGMSLGNHVTQSGFY
                     DEGSESVKASVLGDDVYGRDRRGHIVAIFNAPEGKTVDDPAWSKKIADQLNTFKADHP
                     DQVLDWAGYLRAITGNPDAQRAAQENATVRGTATQDKTRTFVNISLKGDDDDTILNNY
                     KAIESDVRQLDGGNVELAGLQPLANELTGTIGEDQKRAEVLALPLVAVVLFFVFGGVV
                     AAVLPVMVGGLSIAGALGIMRLVAAVTGPVHYFAQPVVTLIGLGIAIDYGLFVVSRFR
                     EEIAEGYDTETAVRRTMMTAGRTVTFSAVLIVASLAGLLLFPQGFLKSLTYAGISSVM
                     LSAILSITVLPACLAVLGKNVDAFGVRTLLRVPFLRNWKPMNWWLNFLADKTQKTKTR
                     EEVENGFWGKLVNRVMKRPVLFAAPILIIMILLIVPLGQLALGGISEKYLPPDNPVRQ
                     AQEDFDRTFPGYRTETVTLVVKNANPAQIAEIKSEAARIPGFTNPTWEERSVSADSDG
                     NLGTKKDPNVTVLENGLQVRNQAAEKISDLRSIQTPKGATIWVGGTPALEQDSIHSLF
                     SKLPLMIVILITTTTLLMFLAFGSLVLPIKAAVMSALTLGSTLGILTWIFVEGHFSHW
                     LNFTATPLTAPVIGLIIAVVYGLSTDYEVFLVSRMVEARERGMSTAEAIRIGTATTGR
                     IITAAALILVVVAGAFVFSDLVMMKYLAFGLIAALLLDATVVRMFLVPSVMKLLGDDC
                     WWAPRWMKRLQNRIGLGEIHLPDERKRPAVREPEEALVGAAAALPPARRPRDPTHPAV
                     EGATRPGAARPAPRAVRPPQPPAPSTAGTTRMPGAGQPRADEPTTTRLSVAAKNAAKA
                     VITNATGIAHRGQRPAPPPPPRQEREIESWLGELRGTVPGPAAQAERPAPSTEATRAM
                     PEAPGEGDEETTAIPVQRPAAGGPPTGPKPDPESTEKLPAQKPGGDERRRGGGVSAQD
                     LLRREGRRL"
     gene            complement(3716..>4133)
                     /locus_tag="VF874_21365"
     CDS             complement(3716..>4133)
                     /locus_tag="VF874_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9178516.1"
                     /translation="IPLVGAVIAGFLAVIVALLSKGFIYALITLGLIIAVQQLEGHVL
                     QPLVMGRAVSIHPLAVVLGISTGGVLAGIVGALLAVPAIAFANSAVRVLMERDEDVGG
                     PGEKPDYDEPVGISAEPDDVGSPTGDGPRAEAGPGR"
BASE COUNT          692 a         1353 c         1448 g          640 t
ORIGIN      
        1 cgccgtcgcc gagctgctct gggagaaggc gcccccggac gcgaggaggc aaatggcatg
       61 agcgtgaccg aacaaataga agagatcccc gccagcccgg ccgcggacat gcaggacacc
      121 cagccggcgc cgcggcgggt gctgctggtc tgggatgcgc caaacctcga catgggcctc
      181 gggtcgatcc tcggtggccg gcccaccgcc gcgtaccgcc cgcgtttcga cgcgctcggg
      241 cggtggctgt tggcgcgcac ggccgaactg gccgccggtc gcagcgacgt gacgctggag
      301 ccggaggcga cggtgttcac caacatcgcc cctggcagcg ccgacgtcgt gcggccgtgg
      361 gtggaggcgt tgcgtaacgt ggggttcgcg gttttcgcga agccgaagat cgacgacgaa
      421 ggcgacgtcg acgccgacat gctcaaccac atcgcgctgc gccacagcga ggggctggcc
      481 agcgtgctgg tggcatccgc ggacgggcag gcgttccggc agccgctgga ggacatcgcc
      541 cgctccggcg tgccggtcca ggtgctcgga tttcgtgaac acgcgagctg ggcgctagcc
      601 tcggatacct tggacttcgt cgacttggaa gacattcctg gcgtattccg ggagccgctg
      661 ccgcgggtgg gcctagattc gctaccggag caaggggcct ggctgcaacc gttccggccg
      721 ctgtcgtcgt tgctgatcgc gcgcgtttag gcaactgtgt agggacgagc aagcccgatg
      781 cgcgctttgg caagagaggt ttaaggagct taagtgttcg cctggtgggg tcgaaccgtg
      841 taccactacc ggttcatcgt gatcgcggtg atggtcgggc tctgcctgct cggcggcttc
      901 ttcgggatga gcctgggcaa ccacgtcacc cagagcggct tctacgacga gggcagcgag
      961 tcggtgaagg cctcggtgct cggcgacgac gtgtacggcc gcgaccggcg cgggcacatc
     1021 gtcgcgatct tcaacgcgcc agagggcaag acggtcgacg acccggcatg gtcgaagaag
     1081 atcgccgatc agctgaacac gttcaaggcc gaccacccgg atcaggttct cgactgggcc
     1141 ggctacctgc gcgcgatcac cgggaacccg gacgcgcagc gggcggcgca ggagaacgcg
     1201 accgtgcggg gcaccgcgac ccaggacaag acgcgaacgt tcgtgaacat cagcctcaaa
     1261 ggcgatgacg acgacaccat tctgaacaac tacaaagcga tcgaatcgga tgtgcggcag
     1321 ctcgacggcg gcaacgtcga attggccggt ctgcagccgc tggccaacga gctgaccggg
     1381 accatcgggg aggaccagaa acgcgccgaa gtgctggcac tgccgctggt ggcggtcgtg
     1441 ctgttcttcg tgttcggcgg cgtggtcgcg gcggtcttgc cggtgatggt cggcggcctg
     1501 tcgatcgccg gggcgctggg catcatgcga ctcgtcgcgg cggtcaccgg accggtgcat
     1561 tacttcgccc agccggtcgt cacgctgatc ggtctgggta tcgcgatcga ctacggcctg
     1621 ttcgtggtga gtcgattccg agaagagatc gccgagggtt acgacaccga gacggccgtg
     1681 cgtcgcacga tgatgacggc ggggcggacc gtcacgttct cggcggtgct gatcgttgcg
     1741 tcattggccg gtctgctgct gttcccgcag ggctttttga agtcgttgac gtacgccggc
     1801 atctcgtcgg tgatgctgtc ggcgattctg tcgatcactg tgctgccggc ctgtctggct
     1861 gtgctgggca agaacgtcga cgcgttcggg gtccggacgt tgctgcgagt tccgttcctc
     1921 cgcaactgga agccgatgaa ttggtggctg aacttcctgg ccgacaagac gcagaagacg
     1981 aagacccgcg aagaggtcga gaacgggttc tggggcaagc tcgtcaaccg ggtgatgaag
     2041 cggcctgtcc tgttcgcggc gccgatcctg atcatcatga tcctactgat cgtcccgctc
     2101 ggtcagctgg cattgggcgg catcagcgag aagtatctgc cgccggacaa cccggtgcgt
     2161 caagcgcagg aggacttcga ccgaacgttc ccgggatacc gcaccgagac ggttacgctc
     2221 gtcgtgaaga acgccaaccc ggcgcagatc gcggagatca agagcgaggc cgcgcgcatc
     2281 cccggcttca ccaaccccac ctgggaagag cgcagcgtct ccgccgactc tgacggcaac
     2341 ctcggcacga agaaggatcc gaacgtcacg gtcctggaga acgggctgca ggtccgcaac
     2401 caggcggccg agaagatctc cgacctgcgc tcgatccaga cgcccaaggg tgcgacgatc
     2461 tgggtgggcg gcactccggc gctcgagcag gacagcatcc acagtctgtt ctccaagctg
     2521 ccgctgatga tcgtgatcct gatcacgacg acgacgctgc tgatgttttt ggcgttcggg
     2581 tcgctggtgc tgccgatcaa ggcggccgtg atgagcgcgc tgacactggg gtcgacgctg
     2641 ggcatcttga cgtggatctt cgtcgagggc cacttctcgc actggctgaa cttcacggcg
     2701 actccgctga ccgcacccgt gatcgggctg atcatcgcgg tggtctacgg cttgtccacc
     2761 gactacgagg tgttcctcgt gtctcgcatg gtcgaagcca gagagcgggg catgtccacg
     2821 gccgaagcga tccggatagg caccgcgacc acgggccgca tcatcaccgc cgcggcgttg
     2881 attctggtgg tcgtcgcggg cgcgttcgtg ttctcggacc tcgtgatgat gaagtacctg
     2941 gcgttcggcc tgatcgcggc gctgctgctg gacgcgaccg tcgtgcggat gttcctggtg
     3001 ccgtcggtca tgaagctgct cggcgacgac tgctggtggg cgccacgctg gatgaagcgg
     3061 ctgcagaacc gcatcgggct gggcgagatc cacctgcccg acgagcgcaa gcggccggcg
     3121 gttcgcgaac cggaggaggc gctggtcggc gccgctgccg ccctgccgcc ggcgcggcgg
     3181 ccgcgcgacc ccacgcaccc cgccgtcgag ggcgcgactc ggcccggcgc ggcacggccg
     3241 gcaccgcggg ccgttcgccc tccgcagccc ccggccccgt cgaccgcagg caccacgcgg
     3301 atgcccggcg ccggtcagcc cagggcggac gaaccgacga ccacgcgact gtcggtcgcg
     3361 gcgaagaacg ccgcgaaggc cgtcattacc aacgcgacgg gcatcgccca tcgcgggcag
     3421 cgacccgcgc cgccgccacc gccacgccaa gagcgcgaga tcgagtcgtg gctcggtgag
     3481 ttgcgcggca ccgttcccgg gcctgcggca caggcggagc gaccggcgcc ctcgaccgag
     3541 gccacgcggg cgatgccgga agcgcccggt gaaggcgacg aggaaaccac ggcgatcccg
     3601 gtgcagcggc cagccgccgg cggaccgccg actggcccga agcccgaccc ggaatcaacc
     3661 gaaaagctac cggcgcagaa gccgggcggg gacgaacgca ggcgtggcgg tggtgtcagc
     3721 gcccaggacc tgcttcggcg cgagggccgt cgcctgtagg ggagccgacg tcgtcgggct
     3781 cggcgctgat gcctaccggc tcgtcgtagt ccggtttctc cccgggtccg ccgacgtcct
     3841 cgtcgcgctc catcagcacg cgaactgcgc tgttggcgaa cgcgatcgcc ggcaccgcca
     3901 gcagcgcccc gacgatgccc gccagcaccc cacccgtcga aatgccgagg accacagcga
     3961 gcgggtgaat cgacacggcc cggcccatca cgagcggctg cagcacgtgc ccctccagct
     4021 gctgcaccgc gatgatcagg ccgagagtga tcagcgcgta gatgaagcct ttggacagca
     4081 gcgccacgat gacagccaga aacccggcga tcaccgcgcc gaccagcggg atg
//
LOCUS       DASWBF010000571         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1050285,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000571 DASWBF010000000
VERSION     DASWBF010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050285"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VF874_21370"
     CDS             <1..1274
                     /locus_tag="VF874_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009479715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX9178517.1"
                     /translation="AKGVLNMLMMSVSAMGVVGILWVLYGYSVAFGNDVLGTGFGGLL
                     GNPTQYWGLKGLIGGTYLQAATDGAPPVNIPLAGTIPQTVFVAFQAMFAIITVALISG
                     ALADRLRFGPWLLFAGIWATLVYFPVAHWVFAFNGVTGEKGGWLANNLHVIDFAGGTA
                     VHINSGAAGLALCIVLGRRRGWPATAMRPHNLPFTMLGAGLLWFGWFGFNAGSAVASN
                     GIAGATFITTFTATATAMFGWLLVERIRDGKGTSLGAASGIVAGLVAITPSCSSVNIL
                     GAIVVGAAAGIICALAVGMKYRFGFDDSLDVVGVHLVGGLVGTLLVGLMLTPESPASV
                     TGTGATGLFYRGGWHQLGVQAIGAFSVMAYSFTVASIIALVIKFTLGLRATAEDEELG
                     IDEAEHAETAYDFASVGGGSALSMADAGKEA"
     gene            1276..1614
                     /locus_tag="VF874_21375"
     CDS             1276..1614
                     /locus_tag="VF874_21375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9178518.1"
                     /translation="MKLITAIVKPFTLEDVKTGLEQTGILGMTVSEVQGYGRQKGHTE
                     VYRGAEYSVDFVPKVRVEVVVDDSAVDKVVDVIVQAARTGKIGDGKVWVSPVEAVVRV
                     RTGERGVDAL"
     gene            1619..>2895
                     /locus_tag="VF874_21380"
     CDS             1619..>2895
                     /locus_tag="VF874_21380"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511305.1"
                     /note="catalyzes the uridylylation or deuridylylation of
                     the PII nitrogen regulatory protein; also involved in
                     adenylylating and deadelnylyating GlnK; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX9178519.1"
                     /translation="MTLQTPDPAAGDPLTSAAGSPRPAKDLVAAIKQLLAGGSRHLDG
                     AALRDALLDLHEFWLTTKATEIGITNTSGFAIVATGGLGRRELLPYSDLDLMLLHDNM
                     PTEAVSSTADQLWYPLWDANIRIDHSVRTVTEALHVAGDDIAVGMAMLEARHIAGDAE
                     LSSLLIGGARRQWRTGIRSRFDELVEHTHARWQRSGQIAHRAEPDLKCGRGGLRDVQL
                     LNALAIAQLADHHTKPNLASPTGSLADAHLALLNVRTELHRISGRGRELLLAQFADEI
                     GAALRIGDRFDLARMLSDAARTIGYYVDAGLRTAANALPRRGFAALRRPVRRPLDEGV
                     VEFGGEVVLARDARPERDPGLILRAAAASATTGLPLSASTLSRLAESAPELRTPWPRE
                     ALNDLLVLLSAGPTAVTTIEALDRTGLWGRLFP"
BASE COUNT          455 a          942 c          986 g          512 t
ORIGIN      
        1 gcgccaaggg tgtgctgaac atgctgatga tgagcgttag cgcgatgggt gtggtgggca
       61 tcctctgggt gctgtacggc tactcggtgg ccttcggcaa cgacgtcctc ggcacgggct
      121 ttggggggtt actcggtaac ccgacccagt actgggggct gaagggtctg atcggcggca
      181 cctatctgca agccgccacg gacggcgccc cgccggtcaa catcccgctc gccggcacga
      241 tcccccagac ggtgttcgtg gcgttccagg cgatgttcgc gatcatcact gtcgcgctga
      301 tttctggcgc gctggccgat cggctgcggt tcggcccatg gctgctgttc gccggaatct
      361 gggcgacgct ggtgtacttc ccggtcgcgc actgggtgtt cgccttcaac ggtgtcaccg
      421 gcgaaaaggg cggctggctc gccaacaatc tgcacgtgat cgacttcgcg ggcggcaccg
      481 cggtgcacat caactccggt gcagcgggtc ttgcgttgtg tatcgtgctc ggacgacgaa
      541 ggggctggcc ggcaacggcg atgcggccac acaaccttcc gttcacgatg ctgggcgccg
      601 gcctgctgtg gttcggctgg ttcggcttca acgccgggtc ggcggtggcg tcgaacggta
      661 tcgcaggtgc cacgttcatc acgacgttca ccgccaccgc gaccgcaatg ttcggctggc
      721 tgttggttga gcgcattcgc gacggtaagg gcacgtcgct gggcgcggca tcgggcatcg
      781 tggccggcct ggtcgcgatc acgccgtcct gctcgtcggt gaacatcctc ggcgcaatcg
      841 tcgtcggcgc ggccgcaggc atcatctgcg cgctggccgt cggcatgaaa taccgcttcg
      901 gatttgacga ttcgctcgac gtggtcggcg tccacctggt cggcggtctg gtcggcacgc
      961 ttctggtggg gctgatgctc actccggagt cgcccgcatc cgtcacgggt accggtgcca
     1021 ctggactgtt ctaccgtggc ggctggcacc aactaggtgt gcaggccatc ggcgccttct
     1081 cggtgatggc ttactcattc accgtcgctt cgatcatcgc cctcgtcatc aagttcaccc
     1141 tcggcctccg cgcgaccgcg gaagacgaag aactcggtat cgatgaggcc gaacacgccg
     1201 aaaccgcata cgatttcgct agtgtcggag ggggctctgc actgagcatg gccgacgccg
     1261 gcaaggaggc atgagatgaa gctgatcact gcaatcgtca agccgttcac cctcgaagac
     1321 gtcaagaccg gcttggagca aaccgggatc ctcgggatga cggtcagcga ggtgcagggc
     1381 tacggccggc aaaagggcca caccgaggtc taccgcggcg cggagtactc cgtcgacttc
     1441 gtgccgaagg ttcgggtcga agtggttgtc gacgactccg ccgtcgacaa agtcgtggac
     1501 gtcatcgtcc aggccgcacg aaccggcaag atcggtgacg ggaaggtgtg ggtgagcccc
     1561 gtcgaagcgg tcgtgcgggt gcgcaccggg gagcggggcg tcgacgcgct gtgacgggat
     1621 gacgctgcaa acacccgatc ccgctgccgg agacccccta acttcggcag cgggatcacc
     1681 ccgtcccgca aaggatttgg tggccgctat caagcagctg ctggcgggcg gttcccgtca
     1741 cctcgacggt gccgccttgc gcgacgcgtt gctcgacctg cacgagttct ggttgaccac
     1801 caaggccacc gagatcggga tcacaaacac gagcgggttt gcgatcgtcg ccaccggcgg
     1861 cctgggccgt cgggagctgt tgccgtactc cgacctcgac ctgatgctgc tgcacgacaa
     1921 catgccgacc gaggcggtga gcagcaccgc cgaccagttg tggtacccgt tgtgggacgc
     1981 caacattcgc atcgaccaca gcgtgcggac ggtgactgag gcgctgcatg tggccggaga
     2041 tgacatcgcc gtgggaatgg cgatgctcga ggcgcgccac atcgccggcg acgccgaact
     2101 gtcttcgctg ttgataggcg gggcgcggcg gcagtggcgt accggaatcc ggtcgagatt
     2161 cgacgaactc gtcgagcaca cccacgcgcg ctggcagcgc agcggccaga tcgcccaccg
     2221 cgccgagccc gacctcaagt gcggccgtgg cgggttgcgt gacgtgcagc tgctcaacgc
     2281 gctggcgatc gcgcagctcg ccgaccacca cacgaaaccc aatctggcca gcccgaccgg
     2341 ttcactcgct gacgcgcacc tggcgttgct caacgtgcgc accgaactgc accgaatatc
     2401 ggggcgcggc cgggagctgt tgctcgccca gttcgccgac gaaatcggcg ccgcgctgcg
     2461 catcggtgac cgcttcgacc tggcccgcat gctcagtgac gccgcgcgca cgatcggcta
     2521 ctacgtcgac gccggattac gcactgccgc aaacgctctg ccccgtcgag gtttcgccgc
     2581 gcttcgccga ccggtacgcc ggccgctgga cgagggcgtc gtcgagttcg gtggcgaggt
     2641 ggtgctggct cgagatgcgc gtcccgaacg cgacccggga ctgattcttc gggctgcggc
     2701 ggcgtcggcg accaccggtc ttccactgtc ggcgtccacg ctcagccggc tcgccgagtc
     2761 cgcaccggaa ttgcgcaccc cgtggccgcg cgaggcgctc aacgacctgc tggtgttgct
     2821 ttccgcaggc ccgactgccg tgacgacgat cgaggcgctg gaccgaacgg gcttgtgggg
     2881 caggctgttt ccgga
//
LOCUS       DASWBF010000572         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_850825,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000572 DASWBF010000000
VERSION     DASWBF010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850825"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VF874_21385"
     CDS             <1..436
                     /locus_tag="VF874_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178520.1"
                     /translation="YDVTTKPNAVVAEFTGGDGGTLALFSEPAAGRGPAEIAKALLDE
                     AYPDAEKDYEIPNAMVGYHLGYGEVADVYPQDAAGDYVRLRVLIMVAVKNDLALVAAA
                     SGPYRQFTPDFGTGQPSGANLQLALDMSNYVNSFTWRGDPPR"
     gene            complement(408..905)
                     /locus_tag="VF874_21390"
     CDS             complement(408..905)
                     /locus_tag="VF874_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX9178521.1"
                     /translation="MAQRDRDESGTARNARPRDALGRPLPRGSQGVPRIPDDLDLPPE
                     ESLRYAQDLLDRGLAFNAHEVLEAAWKNGPEHERTLWQGLAQLAVGFTHVQRGNISGA
                     CALLDRAAARLTATGRPTPYGIDADGLVGHAQRLIADLRAGAEIAPDRLMPRLVISGA
                     DRRAR"
     gene            1002..3149
                     /locus_tag="VF874_21395"
     CDS             1002..3149
                     /locus_tag="VF874_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HEX9178522.1"
                     /translation="MADKTNTARGGGAAPTADTPAAIRNVVLVGPSGAGKTTLVEALL
                     VAAGVLPRAGSVVDGSTVCDFDDAEIRQQRSVGLAVAPLLHDGVKVNLIDTPGYADFV
                     GELRAGLRAADCALFVIAANDGVDEPTKMLWHECDQVGMPRAVVITKLDHARASYDNA
                     LDSAQNAFGDKVLPLYLPTGNGLIGLLSQQHFVYSDGKRGTQEPDSSYADQIEDTRGA
                     LIEGIIEESEDESLMERYLGGEEIDQAGLIEDLEKAVARGSFFPVIPVCSGTGVGTLE
                     LLEVATSGFPSPLEHPLPEVFTPQGAARNSLSCDPAGPLLAEVVKTTSDPYVGRVSLV
                     RVFSGTIRPDATVHVSGHFSQFFGEAFGEGHSHADHDEDERIGPLSFPLGKQQRPAPT
                     VVAGDVCAIGRLTRAETGDTLSDKGEPLVLKPWSMPEPLLPVAVQAHAKTDEDKLSVG
                     LQRLAAEDPTLRIEQNQETHQIVLWCMGEAHAGVVLDGLANRFGVTVDSIELRVPLRE
                     TFGGKAKGHGRHVKQSGGHGQYGVCDIEVEALPEGSGFEFVDKVVGGAVPRQFIPSVE
                     KGVRAQMEKGVAAGYPLVDVRVSLLDGKAHSVDSSDMAFQMAGALALREAAAAAKINL
                     LEPVDTVSVVVPDDLVGAVMSDLSGRRGRLLGTDNVAGNHTLVRAEVPEVELTRYAID
                     LRSLSHGAGSFTRTFTRYEPMPESAAAKVKASA"
BASE COUNT          529 a         1061 c         1164 g          524 t
ORIGIN      
        1 ctacgacgtg acgacgaagc cgaacgcggt ggtcgcggag ttcaccggcg gcgacggggg
       61 caccctggcg ctgttcagcg agccggccgc cggtcgaggg cccgccgaga tcgcgaaggc
      121 gctgctggac gaggcctacc ccgacgccga gaaggactac gagatcccca acgcgatggt
      181 cggctaccac ctcgggtacg gcgaggtggc cgacgtctac ccgcaagacg cggcgggcga
      241 ctacgtacgg ttgcgggtgc tgatcatggt cgccgtcaag aacgatctcg ccttggttgc
      301 tgcggcgtcc ggcccctatc gccaattcac ccccgacttc ggcaccggtc agccgtccgg
      361 cgccaacctg cagctcgcgc tcgacatgtc gaactacgtc aacagcttca cctggcgcgg
      421 cgatccgccc cgctgatcac cagacgcggc atcaagcggt ccggcgcgat ctcggccccc
      481 gcccgcaagt ccgcgatcag tcgctgcgcg tgcccgacga gtccgtcggc gtcaatcccg
      541 tagggcgttg gccgtccggt cgccgtcagc ctggccgcgg cgcggtccag cagcgcgcac
      601 gcgccgctga tgtttccgcg ctggacatgc gtgaagccga cagccagctg cgccagacct
      661 tgccacagcg tccgctcgtg ctcgggtccg ttcttccacg ccgcctccag cacctcgtgg
      721 gcgttgaacg cgagcccgcg gtcgagcaag tcctgcgcgt agcgcaggga ttcttccggc
      781 ggcaggtcga ggtcgtcggg aatccgaggc acgccttgac tgccacgcgg aagtggccgg
      841 ccgagcgcgt cgcgcggacg cgcgttgcgg gcggtgccgg actcgtcgcg gtcgcgttgc
      901 gccatacgcc ggttagtcct tgtgctcggt gggtgcttgg gggaattgtg gactactacg
      961 tccggcggcc aaaggtgact agggactctc ggaggaatgg cgtggcagac aagaccaaca
     1021 cagctcgagg gggtggtgcc gcgccgaccg cggacacccc ggcggccatc cgcaacgtgg
     1081 tcctggtcgg accgtcgggg gccggcaaga ccacactcgt cgaggcactg ttggtggccg
     1141 cgggcgtgct tcccagagcg ggctccgtcg tcgatggaag cacggtctgc gacttcgacg
     1201 acgccgaaat ccggcagcaa cgctcggtgg gtttggcggt ggcgccgctt ctgcatgacg
     1261 gcgtcaaggt gaacctgatc gacacccccg gctacgccga cttcgtcgga gagctgcgcg
     1321 ccggattgcg ggccgcggat tgcgcgctgt tcgtcatcgc cgccaacgat ggcgtcgacg
     1381 agccaaccaa gatgctgtgg cacgagtgcg accaggtcgg catgccgcgc gccgtggtca
     1441 tcaccaagct cgaccacgcg cgggcgagct acgacaacgc gctggactcg gcgcagaatg
     1501 cgttcggcga caaggtgtta ccgctttatc tgccgaccgg gaacgggctg atcgggctgc
     1561 tgtcccagca gcacttcgtg tattcggacg gcaaacgcgg tacgcaagag ccggactcgt
     1621 catacgccga tcagatcgag gacacgcgcg gcgcgctgat cgagggaatc atcgaggaat
     1681 ccgaggacga gtcgctgatg gagcgctacc tcggcggcga agagatcgac caggcgggac
     1741 tgatcgagga cttggagaag gccgtcgcgc gcggctcgtt cttcccggtg attccggtgt
     1801 gcagcggtac cggtgtcgga acgttggagt tgttggaggt ggcgaccagc gggttcccgt
     1861 cgccactgga acatccgctg ccggaggtgt tcaccccgca gggcgccgca cgtaattccc
     1921 tcagctgcga cccggccggc ccgcttctcg ccgaggtggt caagacgacg tcggatccct
     1981 acgtcggcag ggtgagtctg gtccgggtgt tttccgggac catcaggccc gacgcgaccg
     2041 tgcacgtgtc gggccatttc tcgcagttct tcggggaggc cttcggcgaa ggtcacagcc
     2101 acgcggatca cgacgaagac gaacgcatcg ggccgctgtc gttcccgctg ggcaagcagc
     2161 agcggccggc gccgaccgtg gtggccggtg acgtctgcgc gatcggacgg ctgacccgcg
     2221 ccgagaccgg cgacacgctg tcggacaagg gcgagccgct ggtgctcaag ccgtggtcga
     2281 tgcccgagcc gctgctgccc gtcgcggtgc aggcgcacgc gaaaaccgac gaggacaagc
     2341 tctcagttgg cttgcagcgg ttggccgctg aagatccgac gctgcgcatc gagcagaacc
     2401 aggagacgca ccagatcgtg ttgtggtgta tgggcgaggc gcacgccgga gtggtgctcg
     2461 acggtctcgc gaaccgcttc ggcgtcacgg tcgacagcat cgaactgcga gtgccgctgc
     2521 gagaaacctt cggcggcaag gcgaaagggc atggccgcca cgtcaaacag tcaggcgggc
     2581 acggccagta cggtgtctgc gacatcgagg tcgaagcgct gcccgagggg tcggggtttg
     2641 agttcgtcga caaggtcgtc ggcggcgcag taccgcgcca gttcattccc agcgtcgaga
     2701 aaggcgttcg cgcacagatg gaaaagggtg tggccgcggg ttatccattg gtcgacgtcc
     2761 gggtgtcgct gctcgacggc aaggcgcaca gcgtcgactc gtcggacatg gcgttccaga
     2821 tggcgggtgc gctggcgctg cgcgaggcgg cggctgcagc caagatcaac ctcctcgagc
     2881 cggtggacac tgtgtcggtg gtggttcccg acgatctcgt gggcgcggtg atgagcgacc
     2941 tgtcgggtcg gcgcggccgg ctgctgggca ccgacaacgt cgccggcaac cacacgctgg
     3001 tcagggccga ggtgccggag gtggagctga cgcggtacgc gatcgacctg cgctcgttgt
     3061 cacacggcgc aggttcgttc acccgtacct tcacccgcta cgagccgatg ccagagtcgg
     3121 cggcggcgaa agtcaaagcg agcgcttaag actcgacgtt gggggtgcag accaccttct
     3181 tgacctgccc gccgggcagg tcttccaccg cactcgcctc ggcggcggca ctgtccggcc
     3241 cgtagccgcc ggaccacttg tcgtgcttgt cgagagcg
//
LOCUS       DASWBF010000573         3807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1051053,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000573 DASWBF010000000
VERSION     DASWBF010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051053"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VF874_21400"
     CDS             <1..188
                     /locus_tag="VF874_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178523.1"
                     /translation="LPDRDDAVRLWDHVIALTSFPGFAEIKRTLRERPQLIGLGVDAE
                     SVGVEEDWHGGSRRTKK"
     gene            complement(209..1114)
                     /locus_tag="VF874_21405"
     CDS             complement(209..1114)
                     /locus_tag="VF874_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9178524.1"
                     /translation="MGLVDGRVVVVTGAGRGIGRAHALAFAAEGARVVVNDIGVGLDG
                     SPAGGGSPAQEVVDEITAAGGEAITSGANVSEWDGAASLIHTAIDTLGGLDVLVNNAG
                     IVRDRMLANTSEEEWDAVIAVHLKGHFATMRHAAAYWRGLSKEGKSVDARIINTSSGA
                     GLQGSVGQGNYSAAKAGIAAITLVAAAEMGRYGVTVNAIAPSARTRMTETVFADMMAK
                     PDEGFDSMAPENISPLVVWLGSAQSRDVTGRVFEVEGGIVRVAEGWAHGPQVDKGARW
                     DPAELGPVVAHLVAKARPPVPVYGA"
     gene            complement(1127..1906)
                     /locus_tag="VF874_21410"
     CDS             complement(1127..1906)
                     /locus_tag="VF874_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9178525.1"
                     /translation="MTDAGEALPLNFGLAGRVVLVTGGVRGVGAGISAVFAAQGATVV
                     TCARRAVEGLPYEFHSCDVRDADSVAEMVATVVDRHGRLDVVVNNAGGSPYALAADAS
                     PKFHEKIITLNLLGMLHVSQRANAVMQNQPGGGAIVSISSVSGRRPTPGTAAYGAAKA
                     GVESLTQTLAVEWAPKVRVNALAVGMVETEQVELFYGDAESQAAVAATVPLGRLAKPA
                     EIGWAAAFLASDAASYISGTTMAVHGGGEPPPYLSATSAIK"
     gene            1962..2705
                     /locus_tag="VF874_21415"
     CDS             1962..2705
                     /locus_tag="VF874_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX9178526.1"
                     /translation="MTITSAIVEPGIVAVTVDYPPVNAIPSRGWFELADVITAAGDKL
                     DTHVVILRAEGRGFNAGVDIKEMQNTEGFTALIDANRGCFAAFRAVYECAVPVVAAVN
                     GFCVGGGIGLVGNADVIVASDDATFGLPEVERGALGAATHLSRLVPQHMMRRLFFTAA
                     TVDAAMLHHFGSVHEVVPRDQLDEAALTVARDIASKDTRVIRAAKEALNFIDVQRVNS
                     SYRMEQGFTFELNLAGVSDEHRDAFVKRS"
     gene            2702..3583
                     /locus_tag="VF874_21420"
     CDS             2702..3583
                     /locus_tag="VF874_21420"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9178527.1"
                     /translation="MTDKRSTLDDAVAGVRSGMTIGIGGWGSRRKPMAFVRALLRSDV
                     SDLTVVSYGGPDVGLLCSAGKVRRVYYGFVSLDSAPFYDPWFSSARTSGSIEAREMDE
                     GMLRCGLQAAAQRLPFLPIRAGLGSSVPDFWEGELKTVESPYPLADGGRETLIAMPAL
                     QLDVAFVHLNIGDARGNAAYTGVDPYFDDLFLMAAERRYLSVEKVVSTEELVKAAPRQ
                     ALIVNRMMVDSVVEAPGGAHFSTGAADYGRDEKFQQHYAEAAKSPESWNEFVQTYLSG
                     SEDDYQAAVRRFATEAS"
     gene            3583..>3807
                     /locus_tag="VF874_21425"
     CDS             3583..>3807
                     /locus_tag="VF874_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9178528.1"
                     /translation="MYVSRAEVCVTACADLFRDIGEIMVSPMTTVVSIGARLARMTFS
                     PDILLTDGEALLLEDTPAIGASGAVEGWMPF"
BASE COUNT          621 a         1270 c         1258 g          658 t
ORIGIN      
        1 gcctgccgga ccgcgacgat gccgtccggc tgtgggatca tgtcatcgcg ttgaccagtt
       61 tccccggctt cgcggaaatc aagcgcactc tgcgcgagcg gcctcaattg atcggcctcg
      121 gtgtggacgc ggaatcagtt ggcgtcgaag aggactggca cggtggcagc cgccgcacga
      181 aaaagtaggg cgcaggagcg gaagggaatc aggcgccgta caccggtacc ggcgggcgtg
      241 ccttcgccac aaggtgggcg acgacggggc cgagctcggc gggatcccac cgcgcgccct
      301 tgtcgacctg cggaccgtgt gcccatccct cggcaacacg gacgatgccg ccctcgacct
      361 cgaagactcg tccggtgacg tcgcgtgact gggcgctgcc cagccagacc accagcggcg
      421 agatgttctc gggcgccatc gaatcgaatc cctcgtcggg cttggccatc atgtcggcga
      481 agacggtctc ggtcattcgg gtgcgcgccg acggcgcgat cgcgttgaca gtgacgccgt
      541 agcggcccat ttcggcggcg gcaacgagcg taatggccgc gatgccggcc ttggctgcgc
      601 tgtagttgcc ctgcccgacg ctgccctgca ggcccgcacc cgaactggtg ttgatgatcc
      661 gcgcgtcaac gcttttgcct tctttggaca ggccgcgcca atacgccgcg gcgtgccgca
      721 tcgtcgcgaa atggcccttg aggtgtacgg cgatgacggc gtcccattcc tcttcgctgg
      781 tgttggccag catccggtcg cgaacgattc cggcgttgtt gaccaagaca tccaggccgc
      841 ccaacgtgtc gatcgcggtg tggatcaggc tggccgcgcc gtcccactcc gagacgttcg
      901 cgccgctcgt gatggcttcg ccaccggctg cggtgatctc gtcgacgacc tcctgtgccg
      961 ggctgccgcc accggcgggt gagccgtcga gcccaacgcc gatgtcgttc accaccaccc
     1021 gcgccccctc cgcggcgaag gccagggcgt gcgcccgccc gatgccacgt ccggcgcccg
     1081 tcaccacgac cacgcgaccg tcgaccaaac ccatgcccgt ttctccttac ttgatcgcac
     1141 tggtggccga caggtacggc ggcggctcgc cgccgccgtg cacggccatc gtcgtaccac
     1201 tgatatatga cgccgcatcg gaagccaaaa aagctgccgc ccagccgatc tcggcgggct
     1261 tggccagccg gcccagcggc acggtggccg cgaccgctgc ctgtgactct gcgtcgccgt
     1321 agaacagttc cacctgctcg gtctccacca tgccgaccgc gagtgcgttc acccgcactt
     1381 tcggcgccca ctcgacggcc agcgtctgcg tgaggctctc cacgcccgcc ttcgcggcac
     1441 cgtacgccgc ggtgccaggg gtcggtcgcc gcccgctgac gctggagatc gacacgatcg
     1501 cgccgccgcc tggctgattc tgcatcaccg cgttggctcg ctgcgaaaca tgaagcatgc
     1561 caagcagatt cagcgtgata atcttctcgt ggaatttcgg ggacgcgtcg gcggccagcg
     1621 cgtacggcga gccaccggcg ttgttgacca ccacgtcgag gcgcccgtgg cggtcgacga
     1681 cggtggcgac catctcggcc accgagtccg catcgcgcac gtcgcagctg tgaaactcgt
     1741 acggcaaccc ctcgacggca cgtcgggcgc aggtcacgac cgtcgcaccc tgagcagcga
     1801 agacggcgct gatcccggcg ccaaccccgc gcacgcctcc ggtcaccagc accaccctgc
     1861 cggcgaggcc gaaattgagg ggaagcgctt ctccggcatc ggtcactgtg ctagcttacc
     1921 aagcaagtgc ttgcttaggt agcgacccca ggaagactcc catgaccatc acgtccgcca
     1981 tcgtcgagcc aggcatcgtc gccgtcacgg tcgactatcc accggtcaac gcgattccct
     2041 cccgcgggtg gttcgaactc gccgacgtca tcaccgcggc aggcgacaag ctcgacaccc
     2101 acgtggtgat ccttcgggca gagggccgcg gcttcaacgc cggtgtcgac atcaaggaga
     2161 tgcagaacac cgagggcttc accgcgttga tcgacgcgaa ccgcgggtgc ttcgcggcgt
     2221 tccgcgccgt ctacgaatgc gcggtgccgg ttgtcgcggc agtcaacggt ttctgcgtgg
     2281 gcggcggaat tgggctggtg ggcaacgccg atgtgatcgt cgcctccgac gacgctacat
     2341 tcgggcttcc ggaagtggaa cgcggggcgt tgggcgcagc gacgcacttg tctcgccttg
     2401 tgccacaaca catgatgcgg cggctgttct tcaccgccgc caccgtagac gccgcaatgc
     2461 tgcaccactt cggctcggtg cacgaggtgg ttccccgcga tcagctcgac gaagcggctt
     2521 taacggtggc ccgcgacatc gcgtcaaaag acacccgggt gatccgcgca gccaaggagg
     2581 cgctgaattt cattgacgtg caacgcgtca actcaagcta ccggatggag cagggcttca
     2641 cgttcgagct gaaccttgcc ggtgtgtccg acgagcaccg cgacgcgttc gtgaaacggt
     2701 catgaccgac aagcggagca ctctcgacga cgctgtcgcc ggcgtgcgca gcggtatgac
     2761 gatcggcatc ggtggctggg ggtcgcggcg caagccgatg gcgttcgtgc gggctctgtt
     2821 gcgttcggac gtcagcgatt tgaccgtggt ttcgtatggc ggacccgacg tcgggctgct
     2881 gtgctcggcg ggcaaggtgc ggcgggtgta ctacggcttt gtgtcgctgg attcggcgcc
     2941 gttctacgac ccgtggttct cttccgcacg caccagcggt tcgatcgagg cgcgcgagat
     3001 ggacgagggc atgctgcgct gcggattgca ggcggccgcg caacggttac cgttcctgcc
     3061 gatccgcgcg ggcttgggca gctcggtgcc cgacttctgg gagggcgaac tgaagaccgt
     3121 cgagagtccc tatccattgg ctgatggcgg tcgcgagacg ttgatcgcga tgccggcgct
     3181 gcaactcgac gtggcgttcg tgcacctgaa tatcggtgac gcacggggca atgcggccta
     3241 caccggcgtg gacccgtatt tcgacgactt gttcttgatg gccgccgaac gacgttacct
     3301 atcggtggag aaggttgtgt ccaccgagga actcgtcaaa gccgcgccgc ggcaggcgct
     3361 gatcgtcaac cggatgatgg tcgactcggt ggtcgaagcc ccgggaggcg ctcacttctc
     3421 cacgggcgca gcagattacg gccgcgacga gaagttccag cagcactacg cagaggccgc
     3481 gaagtcacct gaatcctgga atgagttcgt gcagacgtac ttgtcaggca gcgaagacga
     3541 ctaccaggcc gcggtgcggc gattcgcaac ggaggcatcg tgatctacgt gagccgcgcc
     3601 gaggtctgcg tgaccgcctg cgcggacctg ttccgcgaca tcggtgaaat catggtcagc
     3661 ccgatgacga cggtcgtgtc catcggggcg cggctggccc gcatgacatt ctcgcccgac
     3721 atcctgctga ccgacggcga ggcgctgctg ctggaagaca cgcccgcgat cggtgcgtcg
     3781 ggcgcggtcg agggttggat gccgttc
//
LOCUS       DASWBF010000574         5602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1051161,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000574 DASWBF010000000
VERSION     DASWBF010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051161"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..130
                     /locus_tag="VF874_21430"
                     /pseudo
     CDS             1..130
                     /locus_tag="VF874_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009474970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(185..412)
                     /locus_tag="VF874_21435"
     CDS             complement(185..412)
                     /locus_tag="VF874_21435"
                     /inference="COORDINATES: protein motif:HMM:NF013676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEX9178529.1"
                     /translation="MEQVRFETPDTLWTTTYLGDALDALRAEGYPVIDEEAAHLTPAQ
                     HDHINFYGTYSFDVETELRREGHRPLRSPAA"
     gene            complement(667..2991)
                     /locus_tag="VF874_21440"
     CDS             complement(667..2991)
                     /locus_tag="VF874_21440"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX9178530.1"
                     /translation="MGVYNSPRERIAASLGDLTGTGSFSVRHTAPTDDLHLEVRGVGP
                     VQLPLSAAQARRLCEVARPARYGRGELTLLNPEVRNTYEIPRSRVRIDKRRWNRTLRP
                     MLDRLGTDLGLAPGARLDAELHSMLVYAPGQFFVSHQDSEKADDMVATLVVTLPGAFK
                     GGSLLIRHGGETVTYRSTKRTLSFVAFYADCRHEIRPVRSGHRVVLTYNLLLRGEPDV
                     CASWDSVDALAAELRAHFATPPPPRWRDEATRPPNRLVYLLDHEYTKRGLCWHRLKGD
                     DAGRAVTLRAAAGRVDCQVVLALAEIRETWDAIPPDRPGRWYDDDSWPDDTDPDQFAL
                     GDLLDSSLALVHCVGPSGEPADPIVAHVPNDEVCATTPSVQLTPYAAEYEGYMGNYGN
                     TIDRWYRRAAIVLWPAEHTFVVHAQASAAWALRRLHERIGQGDQATARTLAKTLIPFW
                     DNTVRGGESAMVATAALRVASGLDDAPLATMLVRPLRLETLTPREMPALAELADHYGQ
                     LWTAELLDTWFSARRSSTVELAEWIESLPPLCEALTATSQPTARGLLARAWAWLDGAL
                     TQACELPAPSRREEELGRLVAPLRAFLAAAAETEDLDLVQTAVAALCGEHSDTLLPTL
                     VQVLRMAQATATMHARSAAGLDAIASHCRIRLRERLTQPPRAADDWSITAPDGCGCDI
                     CVKLTAFLADPAKRLLEWPLAKPGRAHVHQRIDRAELPVGHQTRRVGRPYALVLTKTD
                     ELFDRETRSRRRRQDDLDWLDAQLETARGKGPAW"
     gene            complement(3523..3957)
                     /locus_tag="VF874_21445"
     CDS             complement(3523..3957)
                     /locus_tag="VF874_21445"
                     /inference="COORDINATES: protein motif:HMM:NF040041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HEX9178531.1"
                     /translation="MTRGNPDNLRAAAAAKSAAAKTRAEQGLREMVRRGTPITFRGLA
                     QTAGVSLDFLYRTSDIRRRVEQLRDQQQRNPQAARIEQVDPDHPSSVVRTLSAQLSEL
                     KRRHREEVTALKAALEAAQGENLELRRRLGSRHAASAQPEQG"
     gene            complement(3954..>5602)
                     /locus_tag="VF874_21450"
     CDS             complement(3954..>5602)
                     /locus_tag="VF874_21450"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9178532.1"
                     /translation="QHEEWNLALDPRIPRRLHEPAQGTSLHFHTIEPAWLRIGLQWWF
                     KVSLETGTLTWSSLRAIRSGIAVFSGWLTGRGPTPPPWLSDDPAGVRVLMLEFLGYVR
                     TLTARTGPNKGKPLSDLRVNDIATNVEQFYLFMHDNRDSAARTLNEPGWLRLRSEHAG
                     LWRRGETRRTAVTPGRREVIDDTALSKIMANLHLIGAPINDGGFGDEQAMRIVMLVAR
                     TGRRVSEIRMLDYRPLLPLDRITDPGADDGDGFVAKLRYQQTKIDGAPDTMLVDAEIV
                     AIIEEQQRWVTEHLAGRWALDVTPTYLFLAAKMNRHADRHYTYERVNLLLNEFARKLE
                     IRDSTGRLVDFNRTHRFRHTKATSLLNAGVPLHVVQRYMGHVTPAMTMEYAETLASTH
                     EREFLRYKKITADGRDLRVDPRDLYDMLELDKRTDRILPNGWCLLPPRRSCDKGNACL
                     TCDKFTTDATFLPELKIQQTRTDQLITDRQRTFKTRTGQDMGPENIWLAGRLQEREAL
                     GRIIDAIDRTDSSDGQRHAVRGAGVTARTDMIDKNQEARS"
BASE COUNT          839 a         1753 c         1971 g         1039 t
ORIGIN      
        1 atacggaacc tcggcgacgt atttgagcga agtcgccacc ccacaccgac gcggcttcta
       61 ctcgtcgttc cagtacgtga cgctgaccag cggccaacta ctcgcgctgg gcgtacagat
      121 catcttgcag caagggggtc cccttagcag cggagcggat ctggtccctt ttgccccgac
      181 tggatcaggc ggccggtgaa cggagcgggc ggtgtccctc gcgacggagt tcggtctcga
      241 cgtcgaagga gtaggtgccg tagaagttga tgtgatcgtg ctgtgcaggt gtcaggtgtg
      301 ccgcctcctc gtctatgacg gggtagcctt cggcacgcag cgcgtcgagc gcgtcgccga
      361 ggtaggtagt ggtccacagc gtatccgggg tttcgaacct tacctgctcc attcgtactg
      421 gtcgttctct tgcagttccg tctgtaagag gatgttgggt cattggtatg cggtacccca
      481 ggcaatccca ggccggccgc catggcggca cctgggggca tggccgtgtt cgggggcatt
      541 caggttgagc acctgggggt agcggtccgg cgttgacggt gagcagctcg tcgggcggtc
      601 atagctggtt tggtgcttgt ggcgccgggg cggcacggtg atcacgtcgt tgtaggcgac
      661 ggcctgttac caggctggcc ccttgccgcg cgccgtctcg agttgcgcat cgagccagtc
      721 gaggtcgtcc tggcgtcggc ggcgtgatcg tgtctcccgg tcgaatagct cgtcggtctt
      781 ggtcagcacg agtgcgtacg gccgtccgac gcgccgggtc tggtgcccta ccggcagctc
      841 ggcacggtcg atcctctgat ggacgtgagc tcggcccggc ttggcgagcg gccattccag
      901 cagccgcttc gcagggtcgg cgaggaacgc ggtgagcttg acgcagatgt cgcagccgca
      961 cccatcgggc gcggtgatgg accagtcgtc ggcggcgcgt ggtggttggg tcagtcgttc
     1021 gcggagccgg atacggcaat gcgaagcgat cgcgtcgagg cccgccgccg agcgtgcgtg
     1081 catcgtcgca gtggcttgtg ccatccgcaa cacctgcacc agggtgggca gcaacgtgtc
     1141 ggagtgttcg ccgcacagcg cggccaccgc cgtctggacc aggtcgaggt cctccgtttc
     1201 ggccgctgcg gcgaggaatg cgcgcagcgg cgccacgagc ctgccgagct cttcctcacg
     1261 gcggctgggc gcgggcaact cacacgcctg ggtgagcgcg ccgtcaagcc aggcccatgc
     1321 ccgcgcgagc aggccccgtg cggtcggctg gctagttgcc gtgagggctt cgcacagcgg
     1381 tggcaacgac tcgatccact cggcgagctc gaccgtcgac gagcgccgcg cggagaacca
     1441 ggtgtcgagc agttcggctg tccacagttg cccgtagtgg tcggcaagct cggccagggc
     1501 gggcatctcg cgtggggtca gcgtctcgag ccgaagcgga cgcaccagca tcgtcgccag
     1561 cggcgcgtcg tcgaggccgc tggcgacacg cagcgcagca gtggccacca tggccgattc
     1621 gccgccgcga acagtgttgt cccagaaggg aatcagcgtc ttcgccaacg tgcgcgcggt
     1681 cgcctgatcg ccctgcccga tgcgttcgtg cagccgccgc agcgcccatg cggcagacgc
     1741 ttgggcgtgc accacgaacg tgtgctcggc cggccacaag actattgcgg cgcgccgata
     1801 ccagcggtca atcgtgttgc cgtagttgcc catgtagccc tcgtattcgg ccgcatacgg
     1861 ggtcagctgt accgacggtg tggtcgcgca cacttcgtcg ttgggaacgt gcgcgacgat
     1921 cgggtcggcc ggctcccccg agggtccgac gcagtgaacg agcgccaagc tcgagtcgag
     1981 cagatcgccc agcgcgaact ggtcaggatc ggtgtcgtcg ggccagctgt catcgtcgta
     2041 ccagcgtccg ggtctgtcgg gcgggatggc gtcccaggtc tcccggatct cagcaagcgc
     2101 caacacgacc tggcagtcaa cgcgcccggc cgccgcccga agggtcacgg cgcggccggc
     2161 gtcgtcgccc ttgagccggt gccagcacag cccacgcttg gtgtactcgt ggtcaagcaa
     2221 gtagaccaac cggttcgggg gccgcgtcgc ctcatcgcgc cagcgcggcg gtggcggcgt
     2281 cgcgaagtgt gcccgcagct cggcggccag cgcatcaact gaatcccacg atgcgcaaac
     2341 atccggttcg ccgcgcaaca acaggttgta tgtcagcacg acccggtgtc cagaccggac
     2401 cggccggatc tcgtggcggc agtcggcgta gaacgcgacg aaggacagcg tgcgcttcgt
     2461 cgaacggtag gtcaccgtct ccccgccgtg gcggatcagc agcgagccgc ccttgaacgc
     2521 gcccggcagc gtcactacaa gtgtggcgac catgtcgtcg gccttctccg agtcctggtg
     2581 cgagacaaag aactgtcccg gtgcgtacac cagcatcgag tgcaactcgg cgtccagccg
     2641 cgctccgggc gcgagcccca ggtcggtgcc cagcctgtcc agcatcggcc gcagcgtgcg
     2701 gttccaccgc cgtttgtcga tccgaacccg gctgcgcggt atctcgtagg tgttccgcac
     2761 ctcggggttc agcagggtca gctccccacg tccataacgt gctggccggg ccacttcgca
     2821 gagtcgtcga gcctgggcgg ccgagagcgg aagctgcacc ggcccgactc ctcgcacctc
     2881 gaggtgcagg tcgtccgtcg gcgcggtgtg gcggacgctg aacgagcctg tgccggtgag
     2941 gtcgccgagg ctggccgcaa tccgttcgcg cggcgagttg taaacgccca tcagaccggc
     3001 gcccctatcc tagggctccc gacgagcaat gctgtccggc cgtgtcgctc acagcgccga
     3061 tagtaagtac acagaccaag ggcagcaatt gatcgatcgg caaatacggc taaccaacac
     3121 ccatggcgat tcgctgcggt gcaggacggt ttgcaccgcc accctgtcct gtcggcggcg
     3181 caagctggcg tgggcggcag tgacgctgcc ggcagcagcg atgccggcgg cataggttgg
     3241 cccggcggca ccggcagggc cggtggcacg ggaggcacct ccggcaatcg caagcagcga
     3301 gtttggctgg caaacgacgg caagcgaccc gcagcggcgg taccggtgcc gttggtggca
     3361 gtgccggcac cggcgcgtcg ggtagggggc gccgatggtt ctggcggtga cgtgacactg
     3421 gcggcggtgg ccatggaaca agatatcggc ggggcggggt tgtgttcaaa gctttcggtg
     3481 gcttgatgtt gcgggcgtag aagatcaaca gcgccatcgc gttcagcctt gctcgggttg
     3541 tgccgaggca gcgtgtcgtg atcccaggcg gcggcgcagt tccaggtttt cgccctgggc
     3601 cgcttcgagt gcagccttga gcgcggtgac ttcttcccgg tggcgtcgct tcagttcgga
     3661 cagctgggcg ctgagggtgc ggaccacgct gctgggatga tccgggtcga cctgttcgat
     3721 ccgcgccgcc tgcgggttac gttgctgctg gtcgcgtagt tgctcgacgc gtcggcggat
     3781 gtcgctggtc cggtaaagga aatcgagcga aacaccggcg gtctgtgcca gtccccggaa
     3841 ggtgatcggg gttccgcgtc gcaccatctc acgcaggcct tgttcggcgc gggtcttcgc
     3901 cgcagcgctt ttggctgctg cagcggcgcg gaggttgtcc gggtttccgc gagtcatgac
     3961 cgggcctcct ggttcttgtc gatcatgtcg gtgcgggcgg ttaccccggc tccacgaacg
     4021 gcgtgccgtt ggccgtcgct gctgtcggtc cggtcgatgg cgtcgatgat ccggccgagc
     4081 gcttctcgtt cttgtaggcg tccggccagc cagatgttct cgggtcccat gtcttggcct
     4141 gtgcgggtct tgaaggtgcg ctggcggtcg gtgatgagct gatcggtgcg ggtctgctgg
     4201 atcttcagct cgggcaggaa ggtcgcgtcc gtggtgaact tgtcgcaggt aaggcacgcg
     4261 tttcccttgt cgcaggatcg gcgtggcggc agcaggcacc aaccgttggg cagaatccgg
     4321 tcggtgcgtt tgtccagttc gagcatgtca tagaggtctc gcgggtcgac ccgcaaatcg
     4381 cgaccgtcag cggtgatctt cttgtagcgc aagaattctc gttcgtgcgt cgaggccagg
     4441 gtctcggcgt actccatggt catagccggt gtcacatgac ccatatagcg ctgcacgacg
     4501 tgcagcggga cacccgcgtt gagcaggctt gtggccttcg tgtgccggaa tcggtgggtg
     4561 cggttgaaat ccaccaatcg ccctgtgctg tcacggattt cgagttttcg cgcgaactcg
     4621 ttgagcagca agttgacccg ctcgtaggtg tagtgccgat cggcgtggcg gttcatcttg
     4681 gcggccagga acaggtaggt cggtgtgacg tccagcgccc atcgaccagc gagatgctcg
     4741 gtaacccatc gctgctgttc ctcgatgatg gcgacgattt cggcatcgac gagcatcgtg
     4801 tcgggcgctc cgtcgatctt cgtctgctgg taacgcagct tggcgacgaa cccgtcacca
     4861 tcgtcggcgc cgggatcggt gatccggtcc agcgggagca aaggccggta gtcgagcatg
     4921 cggatctcgc tgacccggcg accggtccgg gcgacgagca tcacgatgcg catggcctgc
     4981 tcgtcgccga acccgccgtc attgatcggc gcgccaatca ggtgcaggtt cgccatgatc
     5041 ttggacaacg ccgtgtcgtc gatgacctcc cggcggccag gagtgacggc ggtgcggcgg
     5101 gtttctcccc gcctccagag cccggcgtgt tcagatcgga gccgtagcca gccgggttcg
     5161 ttcaaagtgc gggccgcgct gtcgcggttg tcgtgcatga acaggtagaa ctgctcgacg
     5221 ttggtggcga tgtcgttgac ccgcaggtcc gacagtggtt tgcccttgtt cggcccggtg
     5281 cgtgcggtca gggtgcggac gtacccgagg aactccagca tcagcacccg gactccggcc
     5341 gggtcgtcgc tgagccaggg cggtggtgtc ggaccacggc cggtgagcca gccggagaac
     5401 acggcgatgc ccgagcggat tgcgcgcagg ctgctccagg tcagcgtgcc tgtttccagc
     5461 gagaccttga accaccattg cagacctata cgcagccacg ccggttcgat tgtgtggaaa
     5521 tgcaggctcg tgccctgtgc cggctcgtgt agccgacgcg ggatccgagg atccagcgcg
     5581 aggttccatt cctcgtgttg cc
//
LOCUS       DASWBF010000575         4886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_577078,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000575 DASWBF010000000
VERSION     DASWBF010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577078"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="VF874_21455"
     CDS             complement(<1..1534)
                     /locus_tag="VF874_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9178533.1"
                     /translation="MAAPQLIIDPATRGVVRLIGGPGTGKTSMLVNAAAAHIASGTYP
                     ESVLLLCPTARAAVRTRRALATALTQSDGPAVIREPLVRTLHSYAFAVLRLAAQRSGD
                     PPPRLVTGTEQDGIIRELLAGDLEDGPDAAVAWPEPLRSALATAGFATALRDLLARCA
                     ERGVDGPQLQRIGRLSGRPEWVAAGQFAQQYEQVMLLRAAAGRAAPQATVPALGAAEL
                     VGAALEAFAHDPGLLTAERDRIRVLLVDDAQHLDPQAARLVGVFAEGAAATVLAGDPN
                     QTVFGFRGADPALLEGDHVVTLTQSHRCAPAVARAVSAVAARLPGRTPGRVIAGAGDE
                     PGEVVVRLATSAHAEAALIADALCRARLIDDVPWSKMAVIVRSLPRAAAVLPRALAAA
                     GVPVRAPASATPPADHPAVRALLTVLAAATGGLDGQQALALVTGPIGRIDPVSLRALR
                     RSLRRSENNDTPRRFEELLIDALTGELKVPAAYAPALRRVRAVLDAAAHAAEEDPNFA
                     LWQ"
     gene            1681..2460
                     /locus_tag="VF874_21460"
     CDS             1681..2460
                     /locus_tag="VF874_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9178534.1"
                     /translation="MTADLHVYRYGPDGPIRVLAVHGLTGHGKRWQTWATRHLPDVTV
                     AAPDLLGHGRSSWDAPWTIDANVDSLAALLDADGGQPAVVVGHSFGGAIALNLAAARP
                     DLVDALVLLDPAAGLRGRRMREIADEMLASPDYTDRDEARADKLSGSWGEVPADDLEA
                     ELDEHLVPWPGGRVGWRMSLPAMMSYWSELARPIVLPRSGTPTTLLRAAKTDPPYVGD
                     ALVVALRDSLGPDFTLVDLDCDHMVPLARPDETAAAVRERL"
     gene            2457..2753
                     /locus_tag="VF874_21465"
     CDS             2457..2753
                     /locus_tag="VF874_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX9178535.1"
                     /translation="MTAVTDDDVELVRTLVSAIPPGKVVTYGDIATAAGLSSPRIVGW
                     IMRTDSVDLPWHRVIRSNGRPAPHLRTRQLELLRAEGVLAVDGRVPLRDVRHAF"
     gene            complement(2727..3470)
                     /locus_tag="VF874_21470"
     CDS             complement(2727..3470)
                     /locus_tag="VF874_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HEX9178536.1"
                     /translation="MLILVAHGTRSAAGVQMAHDLSAAVASRIGPLRTAFVDVLGPNP
                     AEVLAQATGPTVVVPAFLASGYHVHTDLPARIGQSGHVEVSVAPTLGPDPGLAAVMRD
                     RLREVGWRPGDAVVMAAAGSSDPGARHELRIAAQLLARRIGPVHLGYVATGTPRVHDV
                     VGRLRVTGQRRIFIASYLLAPGLFHDRLADCGATAVAAPLGLHPSVIDLLVNRFLHRN
                     GIPRGKVRVDTTWNTVSARRHSECVADVA"
     gene            complement(3464..4609)
                     /locus_tag="VF874_21475"
     CDS             complement(3464..4609)
                     /locus_tag="VF874_21475"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9178537.1"
                     /translation="MEPLKSPTNDPLTGFTIGVTAARRSEELITLLERRGAAVVHAPA
                     IRIIPLVDDDELRRVTTLLIEQPPDVVVVTTGIGFRGWFEAAHGWDVADELMDALAST
                     RILARGPKARGAVRQVGLSEEWSPESEVSPEVLDRLLADGAAGLRIAVQLHGVASEWE
                     PNADICDALTMAGAEVIKVPVYRWEQPEDSRPMDQLIAMIVNAEVDAVSFTSAPAVAS
                     MLQRAKALGCVSELTDALHEQVVAICVGPVTAAPLRRLGVPTTHPERYRLGALARLIT
                     DEVPRRACHFTAGGHHISVRSATVAVDGETRTVPPAAMALLRRLMANPGWVVSREQLL
                     AQLPGGGGDTHAVETAMTRLRSALGAPRAIQTVVKRGYRLAIDPAEC"
     gene            complement(4581..>4886)
                     /locus_tag="VF874_21480"
     CDS             complement(4581..>4886)
                     /locus_tag="VF874_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H) small subunit"
                     /protein_id="HEX9178538.1"
                     /translation="VLLPGGEQAALFLLTDGTLAAVSNIDPFGRAAVMSRGITGDRRG
                     EPTVASPLLKQVFSLADGRCLDDPGGGAQRGGKGQESHALTIYDVRVTDGTVEIAHE"
BASE COUNT          745 a         1743 c         1692 g          706 t
ORIGIN      
        1 cctgccacag cgcgaaatta gggtcctcct cggccgcatg ggccgccgcg tcgagaaccg
       61 cccgcacccg gcgcaacgct ggcgcgtatg cggcgggcac tttcagctcg ccggtgagcg
      121 cgtcgatgag tagctcctcg aatcgccttg gggtgtcatt gttttcactg cgccgcaggc
      181 tccggcgcag tgcgcgcagc gacaccgggt cgatacgccc gatcggcccg gttaccaacg
      241 caagcgcctg ctgcccgtcg agccccccgg tggcggcggc cagcacagtg agcagcgcgc
      301 gcaccgcggg gtggtcggcc ggcggggtgg ctgacgccgg cgcgcgcacc ggaacaccgg
      361 cagcggcaag cgcgcgcggc agtacggccg ccgcccgtgg caacgaccgc acgatcacgg
      421 ccatctttga ccacggcacg tcgtcgatca ggcgcgcacg gcatagcgcg tcggcgatca
      481 aggcagcctc cgcgtgcgcg gatgtcgcga gccggacgac gacctctcca ggctcgtcgc
      541 cggcgcccgc gatgacgcgg cccggcgtgc gacccggaag acgggcggcc acggcactca
      601 ccgcacgcgc gaccgcgggt gcgcagcggt gcgactgcgt cagcgtgacg acgtggtcgc
      661 cttccaacag cgcggggtcg gcaccgcgga acccgaacac ggtctggttg gggtcacccg
      721 cgaggaccgt agcggccgcg ccctccgcga acacccccac cagccgcgcg gcctgcgggt
      781 cgaggtgttg agcgtcgtcg acgagcagca cgcggatgcg gtcccgctcg gcggtgagga
      841 ggcctggatc gtgggcgaac gcctccagcg ccgcgccgac cagttctgcc gcgccgagcg
      901 ccgggacggt ggcctgcggg gcggccctgc ccgcagccgc ccgcagcagc atcacctgct
      961 cgtactgctg cgcgaactgt cccgccgcca cccactccgg ccgcccggag agccgcccga
     1021 tccgttgcag ctgtggtccg tcaacaccgc gttcggcaca gcgcgccaat aggtctcgaa
     1081 gagcggtggc gaaccccgcg gtggccaacg ccgaacgcag cggctccggc cacgccaccg
     1141 ctgcatcggg tccgtcctcg aggtcgccgg ccagcagctc acggattatg ccgtcctgct
     1201 cggtgccggt gaccaggcgc ggcggcgggt caccagagcg ctgcgctgcc agccgcagca
     1261 ctgcgaaggc gtacgagtgc agcgtgcgca ccagcggctc gcggatgacc gcgggaccgt
     1321 ctgattgcgt caacgccgtg gccagcgctc ggcgggtcct gaccgcggcg cgtgccgtcg
     1381 ggcacagcag cagaacggat tccgggtagg tgcccgatgc gatgtgcgcg gccgccgcgt
     1441 tgacgagcat gctggtcttt ccggtgccgg ggccgccgat gagacggacg acgccccggg
     1501 tagccgggtc gatgatcagc tgcggtgcgg ccatacggcg atgacagcac gggggtccga
     1561 caagttcgct tggctggcgc atttgcgact gcctcgatat aaggaacggc gcatggtcgg
     1621 catgggcgag tcgtcttatc acctgcaacc ccgcgggagc cgccctggca cgatcgaggc
     1681 gtgaccgccg accttcacgt ttaccggtac gggcccgacg ggccgatccg agtgctggcc
     1741 gtccacgggc tgaccggaca cggcaagcgg tggcagacct gggccacccg gcacctgccc
     1801 gacgtcacgg tggccgcacc cgatctgctc ggccacggcc ggtcgtcgtg ggacgcgccc
     1861 tggacgatcg acgcgaacgt cgattcactg gcggcgctgc tcgatgccga cggcggacag
     1921 ccggccgtcg tggtgggaca ttcgtttggc ggggcgatcg cgttgaatct cgctgcagcg
     1981 cgacctgatt tggttgacgc gctggtgctg ctggacccgg cggcgggtct gcgcgggcgg
     2041 cgcatgcgcg aaatcgccga cgagatgctg gcctccccgg actacaccga ccgcgacgag
     2101 gcccgcgccg acaagctgtc gggatcctgg ggcgaagtcc ccgcggacga cctggaggcc
     2161 gagctcgacg agcatctggt cccgtggcct ggcggacggg tgggttggcg gatgagcctg
     2221 cccgcgatga tgtcgtactg gagcgagctg gcccgcccga ttgtgttgcc gcgcagcgga
     2281 accccgacga cactgctgcg cgcggccaag accgacccgc cgtacgtcgg cgatgcactg
     2341 gtggtcgcgc tgcgggacag cctgggcccg gacttcacgc tggtcgacct cgactgcgac
     2401 cacatggtgc cgttggcccg gcccgacgag accgccgccg cggtgcggga acggctatga
     2461 cggcggtcac cgacgacgat gtcgagctgg tgcggacgct ggtttccgcg atcccgcccg
     2521 gcaaggtggt cacctatggc gacatcgcca ccgccgcagg gctgtccagc cctcgcatcg
     2581 tcggctggat catgcggacg gactccgtcg acctgccgtg gcaccgggtg atccgctcca
     2641 atggccggcc ggcgccgcac ctgaggaccc gccaactcga actgctgcgc gccgaaggtg
     2701 tgctggccgt cgacggcaga gtgccgctac gcgacgtccg ccacgcattc tgagtgccgc
     2761 cgcgccgaaa cggtattcca cgtggtgtcc actcgcactt tcccgcgtgg aataccgttt
     2821 cggtgcaaga agcggttgac gagcaggtcg atgacacttg gatgcagtcc gagcggggcc
     2881 gcgaccgcgg tcgcaccgca gtcggccaga cgatcatgaa atagccctgg cgccaacaga
     2941 tatgacgcga tgaatatccg gcgctgtccg gttacacgca gcctgccgac gacgtcatgc
     3001 acccgcggcg tgccggtggc cacatagccc agatgcaccg gaccgatgcg ccgcgccaaa
     3061 agctgtgcgg cgattcgcaa ttcatggcgg gcgcctggat ccgacgagcc ggcagccgcc
     3121 atcacgaccg catcgccggg ccgccagccg acttcacgca gccggtcgcg catcaccgcc
     3181 gcgaggcccg ggtcggggcc gagcgtcggt gccaccgaca cctcgacgtg cccgctctgg
     3241 ccgatccggg caggaaggtc ggtatgaacg tgatagcccg acgccaagaa cgcgggtacg
     3301 acgaccgtcg gcccggtcgc ctgagcgagc acctcagcgg ggttgggccc gaggacgtcc
     3361 acgaatgctg ttcgcagcgg cccgattcgg ctcgccaccg ccgcgctcag gtcgtgggcc
     3421 atctgcaccc cggcggcgct gcgggtgccg tgtgcgacca ggatcagcat tcggccgggt
     3481 cgatcgccaa ccggtaaccg cgcttgacca cggtctgtat cgcacgcggc gcgccgagcg
     3541 cagaccgcag ccgggtcatc gccgtctcga ccgcatgagt gtctcccccg ccgccgggca
     3601 actgcgcgag aagctgctcc cgcgatacca cccacccggg attcgccatc agacgcctca
     3661 acaacgccat cgcggccggc ggcacggtcc gagtctcgcc gtcgacggcc accgtcgcgc
     3721 tgcgcacgct gatgtgatga ccgccggcgg tgaaatggca agcgcggcgc ggcacttcgt
     3781 cggtgatcaa ccgggccagc gctccgagcc ggtagcgctc ggggtgggtg gtcggcacgc
     3841 cgagccggcg cagcggagcg gccgtcaccg gtccgacaca gatcgccacg acctgctcgt
     3901 gcagcgcatc ggtgagttca ctcacacaac caagagcctt ggcgcgctgc aacattgacg
     3961 ccactgccgg tgcgctggtg aagctcaccg catccacctc ggcgttgacg atcatcgcga
     4021 tcagctggtc catcggccgg ctgtcttccg gttgctccca gcgatacacg ggcaccttga
     4081 tcacctcggc gcccgccatg gtcagcgcat cgcagatgtc cgcgttgggt tcccactccg
     4141 aggcgacgcc atgcaattgc actgcgatgc gcaatcccgc cgcgccgtcg gccagcaacc
     4201 ggtcgagcac ctcgggcgac acttccgatt ccggactcca ttcctcgctg aggccgacct
     4261 gacggacggc gccgcgcgcc ttggggccac gcgccaggat gcgggtcgac gccagcgcgt
     4321 ccataagctc gtcggcgacg tcccagccgt gagcggcttc gaaccaccct cggaagccga
     4381 tgcccgtcgt gaccacgacg acgtctggcg gctgttcgat cagaagcgtt gtgacgcgcc
     4441 tcaattcgtc gtcgtcgacg agcgggatga tccggatcgc cggcgcgtgc acgacggcgg
     4501 caccgcggcg ctcgagcagc gtgatgagtt cttcggatcg gcgggccgcg gtgaccccga
     4561 tcgtgaaacc tgtcagtggg tcattcgtgg gcgatttcaa cggttccatc agtcactcgc
     4621 acgtcgtaga tcgtcagcgc gtgcgactcc tgacccttcc cccctcgctg cgctccaccc
     4681 cctgggtcgt cgaggcaccg tccatcagca agagagaaga cctgtttgag gagtggtgag
     4741 gccaccgtcg gctcgccgcg gcggtcgccg gtgatgccgc gtgacatcac cgcggccctg
     4801 ccgaacgggt cgatgttgct gacggccgcc aaagttccgt cggtgagcag aaacagcgcc
     4861 gcctgctcgc cgccgggcag cagcac
//
LOCUS       DASWBF010000576         8281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1111130,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000576 DASWBF010000000
VERSION     DASWBF010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111130"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="VF874_21485"
     CDS             complement(<1..106)
                     /locus_tag="VF874_21485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HEX9178539.1"
                     /translation="MNPLNYLYLSALLFTIGAAGVLLRRNAIVMFMCVE"
     gene            complement(103..864)
                     /locus_tag="VF874_21490"
     CDS             complement(103..864)
                     /locus_tag="VF874_21490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9178540.1"
                     /translation="MTTLLADAITRTSTSEAVLFWILGTVAVIGAVGMVTASKAVYSA
                     MFLAMTMIVLAVFYIAQDALFLGVVQVVVYTGAVMMLFLFVLMLIGVDTAESLVETIR
                     GQRIAALLAGLGFGVLLVAGIGSASTAFTGLAQANAEGNVEGLAVLIFTRYLWGFEVT
                     SALLITAAIGAMVLAHRERFDRRKTQRELSQERFAGHGRPTPLPGPGVYARHNAVDIP
                     ALLPDGSYAEASVSKILTPRTMDLTGNGSAEGNGR"
     gene            complement(861..1379)
                     /gene="nuoI"
                     /locus_tag="VF874_21495"
     CDS             complement(861..1379)
                     /gene="nuoI"
                     /locus_tag="VF874_21495"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX9178541.1"
                     /translation="MPKFLDAIAGFGVTLATMFKKPITEQYPEKPGPVAPRYHGRHQL
                     NRYPDGLEKCIGCELCAWACPADAIYVEGADNTEEERFSPGERYGRVYQINYLRCIGC
                     GLCIEACPTRALTMTNIYEMADDNRADMIYGKDKLLAPLQPGMIAPPHPMAPGATDED
                     YYRGNIQAVQPQ"
     gene            complement(1372..2580)
                     /gene="nuoH"
                     /locus_tag="VF874_21500"
     CDS             complement(1372..2580)
                     /gene="nuoH"
                     /locus_tag="VF874_21500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9178542.1"
                     /translation="MIGFGHDPWWLVVGKAVAVFVFLLLTVLAAILIERKVLGRMQMR
                     FGPNRVGPFGILQSLADGIKLGLKEGIIPAGVDKPVYLLAPVISVIPAFMAFAVIPFG
                     PEVSIFGHTTPLQLTDLPVAVLYVLAVTSVGVYGIVLAGWASGSTYPLLGGLRSGAQV
                     ISYEIAMALSFAAVFLYSGTMSTSGIVAAQDRVWFVFLLLPSFLIYVTSMVGETNRAP
                     FDLPEAEGELVGGFHTEYSSLKFALFMLAEYVNMTTVSALATTLFLGGWHAPWPLNMI
                     DGANSGWWPVLWFTAKVWGFLFLFIWLRASLPRLRYDQFMALGWKILIPISLGWVMIV
                     ASVRTLGAEGYHYWAVALFAASIVGGIALVLSVRSLVLSRARSVALDTSTPYSDFPVP
                     PLPEKKAADA"
     gene            complement(2577..4970)
                     /locus_tag="VF874_21505"
     CDS             complement(2577..4970)
                     /locus_tag="VF874_21505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEX9178543.1"
                     /translation="MTATADSDEVVDVEMVTLTIDDVELSVPKGTLVIRAAELIGIEI
                     PRFCDHPLLDPVGACRQCLVEVEGQRKPMASCTIAVTDGMVVHTQFTSEAADKAQHGV
                     MELLLINHPLDCPVCDKGGECPLQNQAMSNGRVDSRFEDVKRTFPKPINISSQVLLDR
                     ERCVLCARCTRFSDQIAGDPFIELLERGALQQVGIYTKEPFDSYFSGNTVQICPVGAL
                     TGAAYRFRARPFDLVSSPSVCEHCASGCAQRTDHRRGVVLRRLAGDDPQVNEEWNCDK
                     GRWAFTYATQPDRITTPLIRGEDGTLVAASWSEALTVAAKGLSAAYGNAGVLVGGRAT
                     LEDAYAYGKFARVVLGSNDIDFRARSQSAEESEFLAARIAGQPITVTYADLEAAPVVL
                     LAGFEPEEESPIVFLRLRKAARKHRTQIYSVAPFATRGLAKMSGTLLQSAPGGEASLL
                     DELQNGEFAEILSASGAVIMLGERLASSPGALSAAVRLADSTGARLVWVPRRAGERGA
                     LEAGALSSLLPGGRPVADPHARSQVSSVWNVTSLPTATGRDADEILAGADAFGALVVG
                     GVEPADLPDPVAAAAALDAAPFVVSLEIRHSAVSERADVVFPVAPVVEKAGTFLNWEG
                     RQRPFEPSLRNTGAFSDMRVLRMIADEMGVDLGLRDAAAARDELAALGVWDGARPEPP
                     HVSPADAPQPGPGQAVLASWRMLLDNGRLQDGEPHLAGTAHRPVARLSAATVAEIGAA
                     DGDLVRVSTETGSVALPLEVTDMPDRVVWVPMNSATSAVHQKLRVTVGAVVTIEVAK"
     gene            complement(4967..6295)
                     /gene="nuoF"
                     /locus_tag="VF874_21510"
     CDS             complement(4967..6295)
                     /gene="nuoF"
                     /locus_tag="VF874_21510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX9178544.1"
                     /translation="MTAETPLTPVLSKFWDDSKSWTLETYREHDGYRALAKALTMGPD
                     TVLQEVKDSGLRGRGGAGFGTGMKWGFIPQDSSGTGAKPHYLVINADESEPGTCKDIP
                     LMFMTPHFLVEGAIIAAYAIRASHAFIYVRGEVVPVLRRLRAAVAEAYEAGYVGRDIL
                     GSGFDLDIVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVSGLYACPTVVNNV
                     ESIASVPPIILNGVDWFRSMGSEKSPGFTLYSLSGHVTRPGQYEAPLGITLRELLDYA
                     GGVRAGHQLKFWTPGGSSTPILTDEHLDVPLDYEGVGAAGSMLGTKALQIFDETTCVV
                     RAVQRWTEFYEHESCGKCTPCREGTYWLAQIYERLETGDGTSADIEKLLDISDTVLGK
                     SFCALGDGAASPIISSIEYFRDEYVAHVEQRGCPFDPRDSMLVAPEGAAV"
     gene            complement(6297..7064)
                     /gene="nuoE"
                     /locus_tag="VF874_21515"
     CDS             complement(6297..7064)
                     /gene="nuoE"
                     /locus_tag="VF874_21515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX9178545.1"
                     /translation="MTVFVSLGAKPEEPSHLRGRETYPEDVRARLEADAAEIIARYPV
                     SRSALLPLLHLVQAEDGCLTPAGVEFCAAQLGLTGAEVTAVATFYSMYRRDPTGDYLV
                     GVCTNTLCAVMGGDAIFDALRDHLDIGNDQTTSDGKITLQHIECNAACDYAPVVMVNW
                     EFFDNQTPESTIELVDALRNGQSVTPTRGLPLCTFRETSRVLAGFADGRPDDGQSGAG
                     APTLAGLREARKNNMQAPAVPTESGTQETDPASTENE"
     gene            complement(7093..>8281)
                     /gene="nuoD"
                     /locus_tag="VF874_21520"
     CDS             complement(7093..>8281)
                     /gene="nuoD"
                     /locus_tag="VF874_21520"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX9178546.1"
                     /translation="QHPSTHGVLRLILEIEGETITEARCGIGYLHTGIEKNLEYRTWT
                     QGVTFVTRMDYLSPFFNETAYCLGVEKLLDITDDIPERTSVIRVMLMELNRISSHLVA
                     LATGGMELGAMSAMFYGFRERELILNVFETITGLRMNHAYIRPGGLAADLPDEAIPQL
                     SELLKLLPKRLRDMENLLKENYIWKARCQGIGYLDLTGCMALGITGPVLRSTGLPHDL
                     RRADPYCGYENYEFDVITDDGCDAYGRFLIRVKEMRESIKIVEQCVDKLRPGPVMISD
                     KKLAWPADLKLGPDGYGNSPEYIAKIMGSSMEALIHHFKIVTEGIQVPAGQVYVAVES
                     PRGELGVHMVSDGGTRPYRVHYRDPSFTNLQAVAAMCEGGMVADAISAVASIDPVMGG
                     VDR"
BASE COUNT         1462 a         2818 c         2638 g         1363 t
ORIGIN      
        1 actccacaca catgaacatc acgatcgcgt tgcgccgcaa caacactccc gcggcgccga
       61 tggtgaacaa cagcgcggac aagtagaggt aattgagcgg attcaccggc cgttcccttc
      121 ggcgctgccg ttgcccgtca aatccatcgt gcgcggcgtg aggatcttcg acaccgacgc
      181 ctccgcgtac gagccgtcgg gaagcagcgc ggggatgtcg accgcgttgt gccgtgcgta
      241 cacgcccgga cccggtaggg gagtgggcct gccgtggccg gcgaaccgct cctgggacag
      301 ctcacgctgg gtcttgcggc ggtcaaagcg ttcccggtgc gccagcacca tcgcaccgat
      361 cgccgcggtg atcagcagtg cactcgtgac ctcgaacccc cacaggtagc gggtgaagat
      421 cagcaccgcc aacccctcga cgttgccctc cgcattcgcc tgagccagac cggtgaacgc
      481 cgtagacgct gagccgatgc cggcgaccag cagcaccccg aagcccagcc cggccaggag
      541 cgcggcaatg cgttgcccgc gaatggtttc caccagcgat tcggccgtgt ccacgccgat
      601 gagcatcagc acgaacagga acagcatcat caccgcgccg gtgtagacca ccacctgcac
      661 gacgcccaag aacagcgcgt cctgcgcgat gtagaacacc gcgagcacga tcatcgtcat
      721 cgccaagaac atcgccgagt agaccgcttt ggatgcggtc accatcccga cggcgccgat
      781 caccgcgacc gtgcccagga tccagaacag cacggcctca ctggtggagg tgcgggttat
      841 cgcatcggcc agcagcgtgg tcattggggt tgcaccgcct gaatgttgcc gcggtagtag
      901 tcctcatcgg tcgcacccgg agccatcgga tgcggcggcg cgatcatgcc tggctgcaag
      961 ggcgccaaca gtttgtcctt gccgtagatc atgtcggcgc ggttgtcgtc agccatctcg
     1021 tagatgttgg tcatcgtcaa cgcccgcgtg gggcacgcct cgatgcacag tccgcagccg
     1081 atgcagcgca gatagttgat ctggtacacc cgcccgtagc gctcgcccgg cgaaaagcgt
     1141 tcctcttccg tgttatccgc gccttcgacg tagatcgcgt cggccgggca ggcccacgca
     1201 cacagctcgc agccgatgca cttctccagc ccgtccggat accggttgag ctggtgccgt
     1261 ccgtgatagc gcggcgccac cggccccggc ttctccggat actgctcggt gatgggcttt
     1321 ttgaacatcg tcgccagcgt gacgccgaac ccggcgatcg cgtcgagaaa cttaggcatc
     1381 tgcggctttc ttctcaggga gcggaggaac cgggaagtcg ctgtatggcg tgctggtgtc
     1441 cagtgcgacg ctccgcgccc tgctgagcac cagacttcgg acggacaaca ccagcgcgat
     1501 accgccgaca atgctcgcgg cgaacaatgc caccgcccag tagtggtatc cctccgcgcc
     1561 cagggtgcgc acggaggcga cgatcatgac ccaccccaac gagatcggaa tcagaatctt
     1621 ccagcccagc gccatgaact ggtcatagcg cagccggggc aggctggcgc gcagccagat
     1681 gaacaagaac aaaaagcccc agactttggc ggtgaaccac agcaccggcc accacccgct
     1741 gttggcgccg tcgatcatgt tgagcggcca cggcgcatgc cagccaccca ggaacagcgt
     1801 ggtggccagc gccgagacgg tggtcatgtt gacgtattcg gcgagcatga acaacgcgaa
     1861 cttcagcgac gaatactcgg tatggaagcc gccgacgagt tcgccttcgg cctcgggcag
     1921 atcgaatggg gcgcggttgg tttcgccgac catcgacgtg acgtagatca agaacgacgg
     1981 cagcagcaag aacacgaacc agacgcggtc ctgggcggcc acgatgcccg acgtcgacat
     2041 ggtgccggag tagaggaaca ccgctgcgaa cgacaatgcc attgcgatct cataggagat
     2101 cacctgcgca cccgatcgca ggccgcccag cagcggatag gtcgaccccg acgcccagcc
     2161 ggccagcacg atgccgtaca cgccgaccga tgtgaccgcc aggacgtaga gcaccgcgac
     2221 cggcaggtcg gtcagctgaa gcggggtggt gtggccgaag atcgacactt cgggtccgaa
     2281 cgggatgacc gcgaacgcca tgaacgcggg aatcaccgaa atgaccggtg ccagtaagta
     2341 gaccggcttg tcgacgccgg cgggaatgat gccctctttc agccccagct tgatgccgtc
     2401 ggcgaggctc tgcaggattc cgaacgggcc cacccggttg gggccgaacc gcatctgcat
     2461 gcggcccagc acttttcgct cgatcaggat cgccgccagc acggtaagca gcaggaagac
     2521 gaagaccgct accgccttgc cgaccaccag ccaccacgga tcgtgcccga aaccgatcat
     2581 ttcgccacct cgatcgtcac caccgcgccc acggtcaccc gcaacttctg gtgcacggcc
     2641 gacgtcgcgg agttcatcgg cacccagacc acgcggtcgg gcatgtcggt cacctccagc
     2701 ggcagtgcga ccgaccccgt ctcggtgctc accctgacca aatcaccgtc cgcggcgccg
     2761 atctcggcga cggtggccgc cgacaaccgc gccaccggcc ggtgcgcggt acccgccaga
     2821 tgcggttcac cgtcctgaag acggccattg tccagcagca tccgccaact cgccaacacc
     2881 gcctgaccgg ggccgggttg cggcgcatcg gccggcgaca cgtgcggcgg ctcgggccgc
     2941 gcgccgtccc acacaccgag cgcggcgagt tcgtcgcgcg ccgccgccgc gtcgcgcagg
     3001 ccgagatcca cacccatttc gtcggcgatc atgcgcagca cccgcatgtc ggagaacgca
     3061 ccggtgttcc gcagtgacgg ttcgaagggg cgttgacgcc cttcccagtt caggaacgtg
     3121 cccgcctttt cgaccaccgg cgccaccggg aacacgacgt cggcccgctc gctgacggca
     3181 ctgtgccgga tctccaggct cacgacgaac ggcgcggcgt cgagcgccgc cgcggcggcg
     3241 acgggatccg gcagatcggc cggttccaca ccgccgacca cgagcgcgcc gaaagcgtca
     3301 gcaccggcga ggatttcgtc agcgtcgcga cccgttgcgg tcggcaggct ggtcacgttc
     3361 caaaccgagc tgacctgtga ccgcgcatgg ggatcggcca cgggccggcc gcctggcagc
     3421 agcgaggaga gtgcgccggc ctccagtgcc ccgcgctcgc cggcgcgccg gggcacccac
     3481 accagacgtg cgccggtgct gtcggccaag cggacggcgg cggacagcgc ccccggagac
     3541 gacgcgagcc gctcaccgag catgatcacc gcgcctgagg cactgaggat ttcggcgaac
     3601 tcgccgtttt gcaactcgtc gagcagcgac gcctcgccgc cgggagcgga ctgcaacagc
     3661 gtgccggaca tcttggcgag cccacgggtg gcgaacggcg cgaccgagta gatctgcgtg
     3721 cggtgcttgc gcgcggcctt gcgcagtcgc aggaacacga tcggcgattc ctcttcgggc
     3781 tcgaacccgg ccagcagcac caccggcgcg gcttccagat ccgcgtaggt gaccgttatc
     3841 ggctgaccgg cgatgcgtgc cgccaagaac tccgactcct ccgcggactg ggaccgagcg
     3901 cggaagtcga tgtcgttgct gcccaacacc acacgcgcga acttgccgta ggcgtaggcg
     3961 tcctccagcg tcgcgcgtcc gccgacgaga acgccggcgt tgccgtaggc cgccgacagt
     4021 cccttggccg cgacggtcag cgcctccgac cacgaggcgg ccaccagcgt gccgtcctcg
     4081 ccgcggatca acggcgtggt gatccggtcg ggttgggtgg catacgtgaa cgcccagcgg
     4141 cccttgtcgc agttccactc ctcgttgact tgtgggtcgt cgccggccag ccggcgcaac
     4201 accacgccgc ggcggtgatc ggtacgctgc gcgcagcccg acgcgcagtg ttcgcacacg
     4261 ctcgggctcg acaccagatc gaacgggcgg gcccggaacc ggtacgcggc tccggtcagt
     4321 gccccgaccg ggcagatctg cacggtgttg cctgaaaagt aagagtcgaa cggttctttg
     4381 gtgtaaatac cgacctgctg cagcgcgccg cgttcgagca gctcgatgaa cgggtccccg
     4441 gcgatctggt cggagaaccg ggtgcagcgc gcacacagaa cgcaccgttc gcggtccagc
     4501 agcacctgcg agctgatgtt gatcggcttg gggaacgtgc gtttgacgtc ctcgaagcga
     4561 gaatccacgc gcccgttgga cattgcctgg ttctgcagcg ggcattcgcc gcccttgtcg
     4621 cacaccgggc agtccagcgg atggttgatc agcagcagct ccatcacgcc gtgctgagcc
     4681 ttgtcggcag cttccgaggt gaactgggta tgcaccacca tcccgtcggt gaccgcaatc
     4741 gtgcacgacg ccatgggctt gcgctggcct tcgacctcca cgaggcactg ccggcatgcg
     4801 ccgaccggat ccagcagcgg atggtcgcag aaccggggaa tctcgatgcc gatcaactcg
     4861 gcggcccgaa tcaccaacgt gccctttggc accgacaact cgacgtcgtc gatcgtcagc
     4921 gtcaccatct cgacgtcaac gacttcatca ctgtcggccg tcgccgtcat accgctgctc
     4981 cttcgggcgc gacgagcatc gagtcgcgcg gatcgaacgg gcaccctcgc tgctcgacgt
     5041 gggcgacgta ctcgtcgcgg aaatactcga tcgacgagat gatcgggctg gccgcgccgt
     5101 cgcccaacgc gcagaacgac ttcccgagga cggtgtccga gatgtcgagc agcttctcga
     5161 tgtcggccga ggtgccgtca ccggtctcca gccgctcgta gatctgtgcg agccagtatg
     5221 tgccttcccg gcacggtgtg cacttgccgc atgactcgtg ctcgtagaac tcggtccagc
     5281 gctgcaccgc acgcaccacg caggtggtct cgtcgaagat ctgcagcgcc ttggtgccca
     5341 gcatggatcc cgcggccccg acaccctcgt aatccagtgg tacgtcgaga tgttcgtcgg
     5401 tcagaatcgg ggtggacgag ccgccaggcg tccagaactt cagctgatgt ccggcccgca
     5461 cgccgcccgc gtagtcgagc agttcgcgca gcgtgatccc cagcggtgcc tcgtactggc
     5521 ctggccgggt cacatgaccc gatagcgaat acagcgtgaa gccaggcgat ttctcgctgc
     5581 ccatcgaccg gaaccagtcg acgccgttga ggatgatcgg cggcacactg gcgatcgact
     5641 cgacgttgtt gacgactgtc gggcaagcgt acagtccgga caccgcgggg aacggcgggc
     5701 gcagcctcgg ctgaccgcgc cggccctcca gcgaatccag cagcgcggtc tcctcaccgc
     5761 agatgtacgc gccggcaccg gcgtggacga cgatatccag gtcgaagccc gagccgagga
     5821 tatcgcggcc cacgtatccc gcttcgtatg cctcggcgac ggcggcgcgc agccgacgca
     5881 gcaccggaac cacctcgccg cgaacgtaaa tgaacgcgtg cgaggcgcgg atcgcgtacg
     5941 ccgcaatgat cgcgccttcg accaggaagt gcggcgtcat gaacatcaac gggatgtcct
     6001 tgcacgtgcc cggctcggac tcgtctgcgt tgatcaccag atagtgcggt ttcgcgccgg
     6061 tcccactcga atcctgagga atgaagcccc atttcatgcc tgtcccgaac cctgcgccgc
     6121 ctcggccgcg caggcccgag tccttgacct cctgaagaac cgtgtcgggc cccatcgtga
     6181 gcgccttggc caacgcccga taaccgtcgt gctcgcggta cgtctccagc gtccacgact
     6241 tggagtcatc ccagaactta ctcagaacgg gcgtcagcgg agtctcggcc gtcatatcac
     6301 tcgttctccg tagacgcggg atctgtttcc tgggttccgg attcggttgg cacggccggc
     6361 gcctgcatgt tgttcttgcg tgcttctcgc aggccggcca gggtgggcgc gcccgctcca
     6421 ctttgaccgt cgtcgggacg cccgtcggcg aaacccgcaa gaacacgcga ggtttcgcgg
     6481 aacgtgcaca gcggcagccc gcgcgtgggt gtgacggatt ggccgttgcg cagcgcgtcg
     6541 acgagttcga tggtggactc cggtgtctgg ttgtcgaaga actcccagtt gaccatcacg
     6601 accggtgcgt agtcgcacgc ggcgttgcac tcgatgtgct gcaacgtgat cttgccgtcg
     6661 ctggtggtct ggtcgttgcc gatgtcgagg tggtctcgca gcgcgtcgaa gatcgcgtca
     6721 ccgcccatca ccgcgcacag cgtgttggtg cacacgccga cgaggtaatc accggtgggg
     6781 tcgcgccggt acatcgagta aaacgttgcg acagcggtga cttcggcgcc ggtcaacccg
     6841 agctgcgcgg cgcagaactc gacgcctgcc ggcgtcaagc aaccgtcctc ggcctgcacg
     6901 agatggagca gcggcagcag cgccgaccgt gacaccgggt agcgcgcgat gatctccgca
     6961 gcgtcggcct ccaggcgcgc gcgcacatcc tccggatagg tttcgcggcc tcgaaggtgg
     7021 ctgggctctt cgggtttggc gcccagcgag acgaagaccg tcatggctgt ctcttcttcg
     7081 cgcgagcgct catcagcgat ccactccgcc catcaccggg tcgatcgagg caaccgccga
     7141 gatcgcgtcg gccaccatgc cgccctcgca catcgccgcg acggcttgca ggttggtgaa
     7201 cgacgggtcg cggtagtgca cccggtaggg gcgggtgccg ccgtcgctga ccatgtgcac
     7261 gccgagctca ccacgcgggg attccactgc tacgtagacc tgtccggcgg gcacctggat
     7321 gccctcggtg acgatcttga agtgatggat cagcgcctcc atcgagctgc ccatgatctt
     7381 ggcgatgtac tcaggcgagt tgccgtagcc gtcgggacct agcttgagat cggccggcca
     7441 cgccagcttc ttgtcgctga tcatcaccgg cccgggccgc agcttgtcga cgcactgctc
     7501 gacgatcttg atcgattccc tcatttcctt gacgcggatg aggaaccggc cgtaggcgtc
     7561 gcagccgtcg tcggtgatga catcgaattc gtagttctca tatccgcagt aggggtctgc
     7621 ccggcgcaga tcatgcggca gcccggtgct gcgcagcacc ggcccggtga tgcccagcgc
     7681 catgcatccg gtcaggtcca ggtagccgat gccctggcag cgggccttcc agatgtagtt
     7741 ctccttgagc aggttttcca tgtcccgcaa ccgcttcggc aacagcttga gcagctcgct
     7801 tagctgcggg atggcctcgt cgggcaggtc ggcggccagc ccgccgggcc ggatgtaggc
     7861 gtggttcatc cgcagcccgg tgatggtctc gaacacgttc aagatgagtt cgcgttcgcg
     7921 gaacccgtag aacatcgccg acatggcacc cagttccatg ccgccggtgg ccagcgcgac
     7981 cagatggctg gagatccggt tgagttccat cagcatcacc cggatcacgc tggtgcgctc
     8041 gggaatgtcg tcggtaatgt cgagcagctt ctccacgcca aggcagtagg cggtttcgtt
     8101 gaagaacggc gacaggtaat ccattcgcgt cacaaacgtc acaccctggg tccaggtgcg
     8161 gtattcgagg ttcttctcaa tcccggtgtg cagatacccg atgccacaac gtgcttcggt
     8221 gatggtttcg ccctcgatct ccaggatcag ccgcaacacc ccgtgggtgg acgggtgctg
     8281 c
//
LOCUS       DASWBF010000577         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_323194,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000577 DASWBF010000000
VERSION     DASWBF010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323194"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1924
                     /locus_tag="VF874_21525"
     CDS             <1..1924
                     /locus_tag="VF874_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX9178547.1"
                     /translation="AAAEPPFRLATYVTDNAGALTAAQRGQVQAAIEKLYNERKIRLW
                     VVYVDDFSSQGAQSWAQSTYRTSDLTGSDALLAVATVDRAYAFLGPDTVNNISAGDVG
                     DLQRTQIEPALRNSDWSGAAVAAANGLNTAGSGMKVPWTALAVVIGVIVLALATLLVL
                     MRWRRRRRRAAEFAAAHRVDPTDPHALASVSLDALDDLSREKVVEVDNAVRTSDNELA
                     LAVEEFGASRTEPFTRAVNNAKAALTQAFRVRQQLDDAIPETPAQRRDLLTRVVVSAA
                     TADRELDAQKEAFEQLRDLVINAPAKLDSLTQQLVDVTARIAPSEQKMEALRNEFDSA
                     ALASVSGNVSEAQKRLTFADQNITRARELAARPIAGQQAELVDAVRAAESALGQARSL
                     LDAVDSAESDINRAAAMLPSAIADIQNGIAQADALLREGNVPRASGLATARGAAVTAV
                     DTAQDTGSTDPLGAFTQLTKADAELDRLLAEVAQEREAAERLARTFDQALFTAQSRVR
                     AVSDFIDTRRGSIGPEARTRLSEATRQLQAAQATRAANVNEAIAYANGASMLAAQAQS
                     LANADVQNAQRAYSGRFGGGNSDMGAMIGGIIIGNILSGGMRGGMGGFGGGWSSTGFG
                     GSSSSGDGFFGGGGRF"
     gene            complement(1880..>2140)
                     /locus_tag="VF874_21530"
     CDS             complement(1880..>2140)
                     /locus_tag="VF874_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HEX9178548.1"
                     /translation="SGGENVYSGEAVHAIVVPREGATLTADAVIEHCRTQIAGYKCPR
                     SVEVRDTPLPMSGAGKVLKRELREAFTRSEPAAAAEEAVARA"
BASE COUNT          366 a          738 c          732 g          304 t
ORIGIN      
        1 tgctgcggca gagccgccgt tccggctggc cacatacgtg accgacaacg cgggcgcgct
       61 gaccgcagcc cagcgcggtc aggtgcaagc cgcgatcgaa aagctctaca acgagcgcaa
      121 gatccggttg tgggtggtct acgtcgacga cttctccagc cagggcgcac agagctgggc
      181 tcaaagtacc taccgaacaa gcgatctgac tggttcggat gcgctgctcg cggtggcgac
      241 cgtcgaccga gcgtacgcgt tcctgggacc cgacaccgtc aacaacatca gcgccggcga
      301 tgtcggagat ctgcagcgca ctcagatcga gcccgcactg cgcaacagcg actggagcgg
      361 cgccgccgtt gccgcggcga acggcctgaa caccgcgggc tccgggatga aggtgccgtg
      421 gacggcactg gccgtcgtca tcggtgtgat cgtgctggcc ctggcgacgc tgctggtctt
      481 gatgcgctgg cgccgacgac gcagacgcgc cgccgaattc gccgcggctc accgggtgga
      541 cccgacagac ccccacgcgc tggcttcggt gtcgctcgac gcgctcgatg atctgtccag
      601 ggagaaggtc gtagaggttg acaacgcggt ccgcaccagc gacaacgaat tggcgctcgc
      661 cgtcgaggag ttcggcgcca gcaggaccga gccgttcacc cgcgccgtca acaatgccaa
      721 agctgctctg acgcaagcct tcagggtccg gcagcaactc gacgacgcga tacccgagac
      781 gccggcccag cgacgggatt tgttgacccg cgtcgtcgtc tccgccgcca cggcggacag
      841 agaactggac gcgcaaaagg aggcgttcga gcaactgcgc gacttggtga tcaacgcgcc
      901 cgccaagctg gactcgctga cccagcagct ggtggacgtc acggcgagga tcgcgccgtc
      961 ggagcagaag atggaggcgc tgcgaaacga attcgacagc gccgctttgg cttcggtgtc
     1021 cggcaacgtg tccgaggcac agaagcgctt gacgttcgcc gaccagaaca tcacccgcgc
     1081 ccgcgagctg gcggcgcgcc cgatcgcggg ccagcaggcc gaactggtgg acgccgtgcg
     1141 agccgcggag tcggcgctcg gacaggctcg gtcgctgctc gacgcagtcg attccgccga
     1201 gagcgacatc aaccgggccg ccgcgatgct gccgtcggcg atcgccgaca ttcagaacgg
     1261 catcgcccag gccgatgcac tgctccgcga aggcaacgtc ccgcgcgcca gcgggctcgc
     1321 taccgcccgc ggggccgccg tcaccgcggt ggacaccgcg caagacaccg gatccaccga
     1381 cccgctcggc gcgttcaccc agctgaccaa ggccgacgcc gaactcgacc ggctgctggc
     1441 cgaagtcgcc caggagcgcg aagccgccga gcggctggct cgcactttcg atcaggcgct
     1501 gttcaccgcg cagtcgcggg tgcgcgcggt gtcggatttc atcgacaccc gccgcggcag
     1561 catcggcccg gaggcacgca cccggctctc ggaggccacc cggcagttgc aggccgcgca
     1621 agccactagg gcggcgaacg tcaacgaagc gatcgcatac gccaacggcg cgtcgatgct
     1681 tgccgcccag gcgcagtcgc tggccaacgc cgacgtgcag aacgcccagc gcgcctactc
     1741 cgggcggttc ggcggcggca actccgacat gggcgcgatg atcggcggga tcatcatcgg
     1801 caacatcctg agcggcggga tgcgcggcgg aatgggcggt ttcggcggcg ggtggagttc
     1861 gactggcttc ggcggttcgt caagctcggg cgacggcttc ttcggcggcg gcggcaggtt
     1921 ctgaccgcgt aaaagcttcg cgcagttcgc gtttgagcac ctttccggcg ccggacatcg
     1981 gcagcggcgt gtcgcggacc tccaccgagc gcgggcactt gtagccggcg atctgcgtgc
     2041 ggcagtgctc gatcacggca tcggcggtga gcgttgctcc ctcccgtggc accacgattg
     2101 catgcacggc ctcgcccgag tacacgttct ctccgccgga
//
LOCUS       DASWBF010000578         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_981421,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000578 DASWBF010000000
VERSION     DASWBF010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981421"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VF874_21535"
     CDS             <1..927
                     /locus_tag="VF874_21535"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX9178549.1"
                     /translation="AADLSRDDAVAILDDADRFAQALVGRDVKKLPTLRGRTIITMFY
                     ENSTRTRVSFEVAGKWMSADVINVSASGSSVGKGESLRDTALTLRAAGADALIIRHPA
                     SGAAQQLAEWTATEDGGPCVINAGDGTHEHPTQALLDALTIRQRLGAIEGKRVVIVGD
                     VLHSRVARSNVLLLHTLGAEVVLVAPPTLLPVGVAGWPVTVSHELDAELPVADAVLML
                     RVQAERMTGGFFPSSREYSVLYGLSERRQAMLPGHAVVLHPGPMLRGMEISFSVADST
                     QSAVLQQVSNGVHVRMAVLFHLLVGADQAVSA"
     gene            924..2216
                     /locus_tag="VF874_21540"
     CDS             924..2216
                     /locus_tag="VF874_21540"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9178550.1"
                     /translation="MSVLIRGVRPYGEGDRVDVLVDDGQIVSVGASLDIPDDADVLDA
                     SGQILLPGFVDLHTHLREPGREYAEDIETGSAAAALGGYTAVFAMANTNPVADSPVVT
                     DHVWQRGQQVGLVDVHPVGAVTVGLAGTQLTEMGLMAAGTGQVRIFSDDGICVHDPLV
                     MRRALEYATGLGVLVAQHAEEPRLTVGAVAHEGPNAALLGLSGWPRAAEESIVVRDAL
                     LARDAGARVHICHASTAGTVEILKWAKARGISITAEVTPHHLALDDTRLASYDGRNRV
                     NPPLRETSDAEALRQALVDGVIDCVATDHAPHAEHEKVCEFANARPGMLGLQTALSVV
                     VQTMVQPGLLSWRDVARVMSENPARIAGLPDQGRPLDVGEPANLTVVDPDATWTIRGA
                     DLASRSDNTPYESMTLPATVTATLLRGKVTARDGKCPA"
     gene            2213..2671
                     /locus_tag="VF874_21545"
     CDS             2213..2671
                     /locus_tag="VF874_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX9178551.1"
                     /translation="MSLSMLMMLMILAVALCLLAFVVIRSMLRGPLRDRHARLRSPQI
                     GGLPPVPDTVGAPVIPATTGRYVGSALAPDRLDRIATGDHDEAVLTRYPEGIMVKRSG
                     FRPIWIPDESIITMQADKGLLTIRWRLPSGVEVETGFRGDADELREAGSE"
     gene            2679..3821
                     /gene="carA"
                     /locus_tag="VF874_21550"
     CDS             2679..3821
                     /gene="carA"
                     /locus_tag="VF874_21550"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX9178552.1"
                     /translation="MSARREEQKAQLVLEDGRVFTGTSFGAVGQTLGEAVFSTGMSGY
                     QETLTDPSYHRQIVVATAPQIGNTGWNHEDAESRGERIWVAGYAVRDPSPRASSWRAT
                     GTLDDELVRQGIVGIAGIDTRALVRHLRTRGSMKAGVFSGDALADTDTLLQRVCAQPP
                     MLGADLAGEVTTANGYIVEPEGPQRFTVAALDLGIKTNTPRNFARRGIRSHVLPSSAT
                     FEQIADLRPDGVFLSNGPGDPATADHVVAVTQQVIGAGIPLFGICFGNQILGRALGRS
                     TYKMTFGHRGINVPVIDHATRRVAVTAQNHGFALEGEAGERFDTPFGTAEVSHTCAND
                     GVVEGVKLVDGQAFSVQYHPEAAAGPHDANYLFDQFVDLMSGEARR"
     gene            3950..>5562
                     /gene="carB"
                     /locus_tag="VF874_21555"
     CDS             3950..>5562
                     /gene="carB"
                     /locus_tag="VF874_21555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX9178553.1"
                     /translation="MPRRNDLNHVLVIGSGPIVIGQACEFDYSGTQACRVLRAEGIQV
                     SLVNSNPATIMTDPEFADHTYVEPITPAFVELVIAQQAQRGNKIDALLATLGGQTALN
                     TAVALYENGVLEKYGVELIGADFEAIQRGEDRQRFKDIVAKVGGESARSRVCFTMEEV
                     TETVGELGLPVVVRPSFTMGGLGSGLAHSVEEVRRMAGDGLAASPSANVLIEESIYGW
                     KEFELELMRDGHDNVVVVCSIENVDPMGVHTGDSVTVAPAMTLTDREYQKMRDLGIAI
                     LREVGVDTGGCNIQFAVDPADGRLIVIEMNPRVSRSSALASKATGFPIAKIAAKLAIG
                     YTLDEILNDITKETPACFEPTLDYVVVKAPRFAFEKFPGADPTLTTTMKSVGEAMSLG
                     RNFIEALGKVMRSLETTKAGFWTGPDPEGGVEGALERLQTPSEGRLYDIELALRLGAA
                     VEKVSEASGVDPWFVEQIAGVVALRIELIDAPVLDADLLRRAKHNGLSDRQIAALRPE
                     LAGEVGVRALRQRLGIRPVFKTVDTCAAE"
BASE COUNT          899 a         1870 c         1948 g          845 t
ORIGIN      
        1 gcggccgacc tgagccgcga cgacgccgtc gcgatcctcg acgacgccga ccggttcgcg
       61 caggcgctgg tgggccgcga cgtcaagaag ctgccgacgc tgcgcggccg gaccatcatc
      121 acgatgttct acgagaactc cacccgcacc cgggtgtcgt tcgaggtggc cggcaagtgg
      181 atgagcgccg acgtgatcaa cgtcagcgcc agcgggtcgt cggtgggcaa gggcgagtcg
      241 ctgcgcgaca cggcgctgac gctgcgcgcg gcgggtgctg acgcgctgat catccggcac
      301 ccggcgtccg gggcggccca gcagctggct gagtggaccg cgacagagga cggcgggccc
      361 tgcgtcatca acgccggcga cggcacccac gaacacccca cgcaggcgct gctcgacgcg
      421 ctgaccatcc ggcagcggct cggcgccatc gagggcaagc gggtcgtgat cgtcggcgac
      481 gtcctgcaca gcagggtcgc gcggtccaac gtgctgctgc tgcacacgct cggcgcggaa
      541 gtggtgctcg tcgcgccgcc cacgctgctg ccggtcgggg tggcgggctg gccggtcacc
      601 gtctcgcacg agcttgacgc cgaacttccg gtggccgacg cggtgctgat gctccgcgtg
      661 caggccgagc ggatgacggg cgggttcttc ccgtcgtcac gggagtactc cgtgctgtac
      721 ggattgtcgg agcgccggca ggcgatgctt cccggccacg cggtggtgct gcaccccggt
      781 ccgatgctgc ggggcatgga gatctcgttc tcggtggccg actcgacgca gtctgctgtg
      841 ctgcaacagg tttcgaacgg cgtgcacgtg cggatggcgg tgctgttcca cttgctggtc
      901 ggcgccgacc aggcggtcag cgcatgagcg tgctgattcg cggggtgcgc ccgtacgggg
      961 aaggggaccg ggtcgacgtg ctcgtcgacg acgggcagat cgtctccgtg ggcgcgtctc
     1021 tcgacatccc cgacgacgcc gacgtgctcg acgcgtcggg gcagatcctg ctgcccgggt
     1081 tcgtggatct gcacacccac cttcgcgaac ccggccgcga gtacgccgag gacatcgaaa
     1141 ccggttcggc cgcagcggca ttgggcgggt ataccgccgt cttcgcgatg gccaacacca
     1201 acccggtcgc cgacagcccg gtcgtcaccg accacgtctg gcagcgcggc cagcaggtcg
     1261 gtctggtcga cgtgcacccg gtcggcgcgg tgaccgtcgg gctggccggc acgcagctca
     1321 ccgagatggg gctgatggcc gcggggacgg gccaggtgcg gatcttctcc gacgacggca
     1381 tctgcgtgca cgacccgctg gtgatgcgcc gcgcgctgga gtacgcgacc ggcctgggtg
     1441 tgctggtcgc ccagcacgcc gaggagccgc ggctgaccgt cggcgcggtc gcacacgaag
     1501 ggccgaacgc cgccctgctg ggtctgtccg gctggccacg cgccgccgag gagtcgatcg
     1561 tcgtgcgtga tgctctgttg gcgcgcgacg ccggggcgcg agtgcatatc tgccacgcgt
     1621 ccaccgcagg cactgtcgaa atcctgaaat gggccaaggc acgaggtatt tcgatcaccg
     1681 ctgaggtcac accgcatcac cttgcgctcg acgacacccg gctggccagc tacgacggcc
     1741 gcaaccgggt caacccgccg ctgcgcgaga ccagcgacgc cgaggcgttg aggcaggcgc
     1801 tggtcgacgg ggtgatcgac tgcgtggcga ccgaccacgc accccacgcc gaacacgaga
     1861 aggtctgcga gttcgccaac gcccggcccg ggatgctcgg gctgcagacc gcgctgtcgg
     1921 tggtggtgca gacgatggtt cagcccggac tgctgagctg gcgcgacgtg gcgcgggtga
     1981 tgagcgagaa cccggcgcgc atcgcgggcc tgcccgatca gggccgccca ctcgacgtcg
     2041 gcgagccggc gaacctcacc gtcgtcgacc ccgacgccac gtggacgatc cgcggcgccg
     2101 atctggccag ccggtcggat aacacgcctt acgagtcgat gacgctgccc gcgacggtga
     2161 ccgcaaccct gctgcgcggc aaggtgaccg cccgcgacgg gaagtgcccg gcatgagcct
     2221 gtcgatgctg atgatgttga tgatcctcgc ggtggcgctg tgtcttctgg cattcgtggt
     2281 gatccgctcg atgctgcgcg gaccactgcg ggaccgccat gcgcgcctgc gctcgcctca
     2341 gatcggcgga ctgccgcccg tgccggacac ggtgggcgcg ccggtcatcc cggcgaccac
     2401 cggccggtat gtcggcagcg cgctcgcgcc cgaccggctg gaccgcatcg ccactggcga
     2461 ccacgacgag gccgtgctga cgcgctaccc ggaaggcatc atggtgaagc gcagcggttt
     2521 ccgcccgatc tggattcccg acgagtcgat aatcacgatg caggccgata aaggattgct
     2581 cacaatccgg tggcggctgc cgtcgggcgt ggaggtcgag acgggttttc gcggcgacgc
     2641 cgacgaattg cgagaggctg gcagtgagta aagcagcgat gagcgctcgt cgcgaagagc
     2701 agaaggcaca gttggtgctt gaggacggcc gggtgttcac cggcacgtcg ttcggtgctg
     2761 tcggccagac gctgggcgag gccgtcttct ccaccggcat gtcgggatac caggagacgc
     2821 ttaccgatcc gagctaccac cggcagatcg tcgtcgcgac ggcaccgcag atcggcaaca
     2881 ccggctggaa ccacgaggac gccgaaagcc gcggcgagcg gatctgggtc gccggatacg
     2941 ccgttcgcga cccgtccccg cgggcgtcga gctggcgggc caccggcacc ctcgacgacg
     3001 agctggtccg ccagggcatc gtcggcatcg cgggcatcga cacccgcgcc ctggtgcggc
     3061 acctgcgcac ccgcggctcg atgaaggccg gcgtcttctc cggcgacgcc cttgccgaca
     3121 ccgacactct gctgcagcgg gtgtgcgccc agccgccgat gctgggcgct gacctcgccg
     3181 gcgaagtcac caccgccaac ggctacattg tcgaacccga aggcccgcaa cggtttaccg
     3241 tagcggcgct tgacctcggc atcaagacca acaccccgcg caacttcgcc cgccgcggca
     3301 tccgcagcca cgtgctgccg tcgtcggcga cgttcgagca gatcgccgac ctgcgacccg
     3361 acggcgtctt cctgtcgaac ggcccaggcg atccggccac cgccgatcac gtcgtcgcgg
     3421 tcactcagca ggtgattggc gcggggattc cgttgttcgg catctgtttc ggcaaccaga
     3481 ttctcggccg cgcgctcggg cggtccacgt acaagatgac gttcgggcac cgcggcatca
     3541 acgtcccggt gatcgaccac gccacccgac gggttgcggt gaccgcgcag aaccacgggt
     3601 tcgcattgga gggcgaggca ggggagcggt tcgacacgcc gttcggcacc gccgaggtca
     3661 gccacacctg cgccaacgat ggcgtagtcg aaggcgtcaa actcgttgac ggacaagcat
     3721 tttcggttca ataccaccct gaggcggccg ccggcccaca cgacgcgaat tatctgttcg
     3781 accagttcgt cgacctgatg tcgggggagg cgcggcgatg accggggggc gtggatcgtc
     3841 gagtctgcgg atttgtacgc cacatcggcg tgtcgcgtac gaaaccgcag agtcgggttc
     3901 cagcccacaa ctgcgcccgt tgacgtcggc gcgagaagtg gtgcgctaga tgccgcggcg
     3961 caatgacctc aaccacgtcc tggtcatcgg ctccgggccg atcgtgatcg gccaggcttg
     4021 cgaattcgac tactccggca cgcaggcgtg ccgtgtgctg cgcgccgagg gcattcaggt
     4081 cagcctggtc aactcgaacc cggcgacgat catgaccgac cccgagttcg ccgaccacac
     4141 ctacgtcgag ccgatcacgc cggccttcgt ggagctggtg atcgcacaac aagcccagcg
     4201 cggcaacaag atcgacgcgc tgctggccac actcggcggg cagaccgcgc tgaacacggc
     4261 ggtcgccctc tatgagaacg gcgtgctgga gaagtacggg gtcgagctga tcggggcgga
     4321 cttcgaggcg atccagcgcg gcgaagaccg gcaacggttc aaggacatcg tcgccaaggt
     4381 cggcggcgaa tccgcccgct cccgagtgtg tttcacgatg gaggaggtca ccgagaccgt
     4441 cggggagctc ggtttgccgg tagtggtgcg gccgtcgttc acgatgggcg gcctggggtc
     4501 gggcctcgcg cacagcgtcg aggaagtgcg ccggatggcc ggcgacggcc tggccgcatc
     4561 cccgtccgcc aacgtgctga tcgaggaatc gatctacggc tggaaggaat tcgagctcga
     4621 gctgatgcgc gacggccacg acaacgtggt cgtggtctgc tcgatcgaga acgtcgaccc
     4681 gatgggtgtg cacaccggcg actcggtgac cgtcgcgccc gcgatgacac tgaccgaccg
     4741 cgaataccaa aagatgcgtg acctcggtat cgcgatcctg cgggaggtcg gcgtcgacac
     4801 cggcggctgc aacatccagt tcgcggtcga cccggccgac ggccgactga tcgtcatcga
     4861 gatgaacccg cgcgtgtcgc ggtccagcgc gctggcctcg aaggccaccg gcttcccgat
     4921 cgccaagatc gccgccaaac tcgcgatcgg ctacacgctg gacgagatcc tcaacgacat
     4981 caccaaggaa accccggcct gcttcgagcc aacgctggac tacgtggtgg tcaaggcgcc
     5041 gcggttcgcg ttcgagaagt tccccggcgc tgacccgacg ctgaccacca cgatgaagtc
     5101 ggtcggtgag gcaatgtcgt tgggtcgcaa cttcatcgag gcgctcggca aggtgatgcg
     5161 gtcccttgag acgacgaagg cgggcttctg gacgggaccc gaccccgagg ggggtgtcga
     5221 gggtgcgctc gagcggctcc agacgccgag cgagggccgg ctctacgaca tcgaactcgc
     5281 gctgcggcta ggcgccgccg tggaaaaggt gtccgaagcc tccggtgtcg acccgtggtt
     5341 cgtcgaacag atcgcggggg tcgtcgcatt gcgaatcgag ctgatcgacg ctccggtgct
     5401 cgacgctgac ctgctgcgcc gcgccaagca caacggcctg tccgaccgcc agatcgcggc
     5461 gctgcgaccg gaattggcgg gcgaggtcgg tgtccgtgcg ctgcgtcagc gcctaggcat
     5521 ccgcccggtg ttcaagaccg tcgatacctg cgccgcagaa tt
//
LOCUS       DASWBF010000579        18252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1111666,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000579 DASWBF010000000
VERSION     DASWBF010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18252
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111666"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..791
                     /locus_tag="VF874_21560"
                     /pseudo
     CDS             115..791
                     /locus_tag="VF874_21560"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
     gene            891..1979
                     /locus_tag="VF874_21565"
     CDS             891..1979
                     /locus_tag="VF874_21565"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9178554.1"
                     /translation="MPYDHVAPADTFEQAVKILDHEGFRKEQADALAEGRYIGLGFSA
                     YIEPTAPSMGYFATEGATIRMEPTGKINVYLNGGSCGNSIETTVVQLTADALGAGIDD
                     VATIQGDTAVTPYGAGTQGSRSGPMTAGAVNEAGTMLRKHIVAMAAHRLGVDETDIEL
                     GGSRASVREDPSKSVSFADLAYRAYYEPHHLPHETSASLEATARFTSRAPIHWANATH
                     ACTCEVDVKTGHVKLTRYIVSEDVGPMINPNVVEGQIAGGTVQGIGGALLENFVYDEH
                     GNPLASTFVDYLLPTATEVPSIEFGHIEIPGPGVGGYKGVGEGGAIGSTPAVINAIND
                     ALAPLGVTLTRLPASPAAIVDLIEQATR"
     gene            1990..2628
                     /locus_tag="VF874_21570"
     CDS             1990..2628
                     /locus_tag="VF874_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9178555.1"
                     /translation="MELVNEFRVPVSVDQAWEVLTDVERVAPCIPGAQLLSVNGDEFT
                     GAVKVKVGPITVQYKGKASFEEKDVSARRAVITAHGKETRGQGHAAAIVTAELKDEGD
                     HTSCVLTTDLSISGKAAQFGRGVLADVAGKLIGQFAQRLEADVLAGATASATAETAES
                     QPVSAPTQPEDAASIDLLSLVAVPLAKRHIPPVGTGLVIGVVIGYLLGRRRS"
     gene            2625..3512
                     /locus_tag="VF874_21575"
     CDS             2625..3512
                     /locus_tag="VF874_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9178556.1"
                     /translation="MKSAPFDYHRPDSIAAATQMLAEFGQDAKVLAGGQSLVPMLALR
                     LAFFDHLIDISRLDELKGIERQNGAVWIRAGTTDAIVGTDGEISAAVPLLTRVTPFVG
                     HFQIRSRGTLGGSIAHADPAGEYPAVALALDAEMEAASPSGRRQIPAAEFFTGLWETA
                     LEPDEVLTGVRFPVWTGRCGFAVQEFARRHGDFAIAGAVMAVELDDHDHVSRCAIGLL
                     GLGSTPSRAAEAEAAVVGRPVADVDADKVGALAITGLDDIPADLQGSASYRSRVGAEL
                     VARAWTEAITEASTEAVNA"
     gene            3505..3978
                     /locus_tag="VF874_21580"
     CDS             3505..3978
                     /locus_tag="VF874_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9178557.1"
                     /translation="MHERPVRVSVNGHDTEAIVEPRVTLADFLRERCGLTGTHLGCEH
                     GVCGACTVLLDGAAVRSCLIFAVQVDGMEVTTVEGIASRDGELSPVQSAMRECHGLQC
                     GFCTPGFVMSITALLHDNPHPTEEEIREGLSGNFCRCTGYQSIVNAVHRAAEATC"
     gene            complement(3975..5233)
                     /locus_tag="VF874_21585"
                     /pseudo
     CDS             complement(3975..5233)
                     /locus_tag="VF874_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245827.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
     gene            complement(5251..6090)
                     /locus_tag="VF874_21590"
     CDS             complement(5251..6090)
                     /locus_tag="VF874_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEX9178558.1"
                     /translation="MAGRVEGKVAFVTGAARGQGRSHAVRLAQEGADIIAVDVCKPIV
                     ENTSIPASTPEDLAETADLIKGLDRRVVTAEVDVRDYDALKAAVDSGMEQLGRLDVIV
                     ANAGIGNGGDTLDKTTEHDWQEMIDVNLSGVWKSVKAGVPRIIAGGRGGSIVLTSSVG
                     GLKAYPQCGNYVAAKHGVVGIMRSFAVELGQHMIRVNSVHPTHVNTPMIMNEGTWKLF
                     RPDLENPGPDDMAPICQMFHTLPIPWVEPEDVSNAVLFFASDEARYLTGVTLPVDAGS
                     CLK"
     gene            complement(6156..6671)
                     /locus_tag="VF874_21595"
     CDS             complement(6156..6671)
                     /locus_tag="VF874_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9178559.1"
                     /translation="MTHDYALDTGLLILRLVLGLTMAAHGLNKFFGGGRIPGTARWFE
                     SIGMKPGTLHARIAALTETSAGLGLAAGLLTPIPAAGFVALMLVAAWTVHRPNGFFIV
                     KEGWEYNLVLALSAVAVATIGAGRFSLDWLIFKDNWMDGWAGLAISAGLGLAGGIGQL
                     VIFYRPPVKAD"
     gene            complement(6691..6870)
                     /locus_tag="VF874_21600"
     CDS             complement(6691..6870)
                     /locus_tag="VF874_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9178560.1"
                     /translation="MYVCLCKGVTSQTVVDAVADGASTTNQVAKACGAGSDCGRCRRT
                     VQAIIKAALVSPETR"
     gene            complement(6886..7524)
                     /locus_tag="VF874_21605"
     CDS             complement(6886..7524)
                     /locus_tag="VF874_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HEX9178561.1"
                     /translation="MTNFEGADTRSLVIASDYRVPDPARVPRLLEKRKSALAEIGAHH
                     VFVHASTNDPGRVLVSIVVHNHEPIVDLLRSRVFFEWFDAVGVEDIPAVFAGALVDGL
                     DLVDSTTPTPPSVVVAAMTSVGDVTTLIEHVHLALDSFKAAGIEKTWIFQAFDDPREV
                     MILQQIDNEDDARRWIEHPDAAAEWMERAGMGAYPPLFVGKFLHMMRIDENL"
     gene            complement(7517..7819)
                     /locus_tag="VF874_21610"
     CDS             complement(7517..7819)
                     /locus_tag="VF874_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178562.1"
                     /translation="MIKTGTRLKSQVCDTEVIVIKIGDGLDDLRAGGVPMREIGADAA
                     SDAVLDQNFSGGNVMGKRYVDETGAEVLVTKAGAGTLAIGDTPLSIKEAKPLPASD"
     gene            complement(7816..9315)
                     /locus_tag="VF874_21615"
     CDS             complement(7816..9315)
                     /locus_tag="VF874_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX9178563.1"
                     /translation="MTISLLLEMASSGDPDRGALVSDGTRLTVGELSALADGGAGVIA
                     ASGAGSIAYVGTGGAMLPLLIFSSARAARPFTPLNYRLSAEGLHELIARLPEPLVVFD
                     DDYRDMVEGAGVKSFSCEDFVTMARTAEPAAEFADPDDVAIMLFTSGTTSRPKAVELS
                     HNNLTSYVMGTVEFASADPGDAALICVPPYHIAGVGAALSNVYAGRKMVYLTRFDPKE
                     WIRLVRDEGVTAATVVPTMLDRIVSELEKHPTPLPTLCTLAYGGSKVALPLVRKALDL
                     LPGVGFVNAYGLTETSSTIAVLTPEDHRTAHGATDGTVRRRLGSVGQPVPGIEVEIRG
                     DDGAVLGPGEVGELFVRGDQVSGKYAEIGSVLDEDGWFPTRDVAALDDEGYLFITGRS
                     DDTIIRGGENIAPAEVEDVLVEHPSVRDVAVVGVEDSEWGQIIVAVVVPTEGAEPDPE
                     GLRAHVRKSLRGSRTPDRIVFRDELPTTPTGKVLRRDIVDELLEKKGQS"
     gene            complement(9323..9874)
                     /locus_tag="VF874_21620"
     CDS             complement(9323..9874)
                     /locus_tag="VF874_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal"
                     /protein_id="HEX9178564.1"
                     /translation="MTADEAPPVEDIDVPEAAPKRRLVGNIVPAVWIVLVVASVSLLA
                     VLLYTQYRPDREMNDSTKQSAIDAATEGAVAVLTYSSGNFDRDVSSARSHLTGDFLAQ
                     YDQYIQTAVAPVAKQKSINTSATVVRNAVSELRPDSADVLLYINQTTMAAGDPAPSQA
                     ASSVMVKLTKVDGKSLISSFNPV"
     gene            complement(9871..10626)
                     /locus_tag="VF874_21625"
     CDS             complement(9871..10626)
                     /locus_tag="VF874_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178565.1"
                     /translation="MGDTKKPSAQSGESVTTDVSASDTEPDSPTEESSTDLQAEIEQA
                     EAEAAEAEAAAAAARARVRAIRSGRLDGEAETDGEAEQAQRPWYRRRPRWSTVAKAIA
                     ALVIVGSLAGSGVILWQHHTKSAQRERSEEFAAAAKEGIVALTSLNFNHARDDVQRII
                     DNSTGSFRDDFQARADDFVKVVETSKVVAQGTVKATAVQSMSDDTAVVLVVAEEQITN
                     SAGAKKDPRVLRLSVTVTRDGDRFKLAKVELVP"
     gene            complement(11024..12448)
                     /locus_tag="VF874_21630"
     CDS             complement(11024..12448)
                     /locus_tag="VF874_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HEX9178566.1"
                     /translation="MVLSAGDRFGDAEAVVDGPLRLSFAELTERIRRAAGAFAALGVQ
                     KGDRVAVWAPNSAEWIIAAFGLLTAGGVLVPVNTRFKTEEAGDIIRRSGAKAVLVDTG
                     FFAGFLGQEFDVPAGVPAIDLKSDFLGSGQPFEHEVNGLKGTDTADIIYTSGTTGRPK
                     GAMMHHLQTLRMYEEWATLADLREGDRYLMVNPYFHTFGYKAGLIASFLRGATMLPVA
                     VFDIDRVVELIESEQITMLPGPPTLYHSLLTVGDKSRLSSLRAGVTGAADIPVELVRR
                     VHEELPFQTLMTGYGLTEAGNVTLSRPGDSFDHVATTAGLPCDGVDVRIADDGEVLVR
                     GYGVMKGYFDDPVATADAIDDQGWLHTGDLGKFDGSGRLRIVGRKKDMFIVGGFNAYP
                     AEIEGFLLEHPAVEQAAVIGVPDERLGQVGKAFVVAKGEVTGDELISWSRDRMAGFKV
                     PRFVEFLDALPLNATGKVQKDRLR"
     gene            complement(12481..13671)
                     /locus_tag="VF874_21635"
     CDS             complement(12481..13671)
                     /locus_tag="VF874_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9178567.1"
                     /translation="MGQLSHRVDIPFPLFDADNHLYETEESLTKFLPEEYKDVVQYVQ
                     VNGRTKIALRGQISNYIPNPTFSVVAKPGAWEEYFKYGNPDGKSKRELFGEPMRSIPA
                     FFEPGPRLELMNELGLDRSLMFPTLASLVEERLRDDPVAIHVIVHALNEWLDDVWGFN
                     YQNRIFTTPVITLPIVEKAIEELDWVVKRGARVILIRPAPVPGFRGPRSFALPEFDPF
                     WQKCVEHDVLVAMHSSDSGYSRYTSEWDGAAQEMLPFQTNAMAILNEWRPVQDAVASW
                     VIHGALFRFPTLKVAVIEAGSKWMFPLLDSMAEVYKKAPEAFPGNPIEEIKNRIHVSP
                     FYEEGIDDLINLIGVDRVLYGSDYPHPEGLAEPTHYVTALEHLSVDDQAKIMGGNLSR
                     LVTV"
     gene            complement(13707..14881)
                     /locus_tag="VF874_21640"
                     /pseudo
     CDS             complement(13707..14881)
                     /locus_tag="VF874_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            14943..15665
                     /locus_tag="VF874_21645"
     CDS             14943..15665
                     /locus_tag="VF874_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020109471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9178568.1"
                     /translation="MVDLELDDGLATLTIDRPHARNAIALDTMAELEKVLDALDSSSG
                     ARALVIKGAGDKAFVSGGDLKELAAIRTTEEASAMAWRMRTICDRLAQFPAPVIAALN
                     GHAFGGGAEVAVAADIRVAADDIKIAFNQVSLEIMPAWGGAERLAALVGKGKALMLAG
                     TGTVLDAAEAERIGLVDRVVTRASFDESWQALARSLANRPAGEIKRVIAGVSPDEAVA
                     YFARLWVGDEHWQAADRVMRRR"
     gene            15744..16067
                     /locus_tag="VF874_21650"
     CDS             15744..16067
                     /locus_tag="VF874_21650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019305703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178569.1"
                     /translation="MHHVRYTLTAENPIWAEIYYLDHEPAVFADWSHKPYEFMPNVEA
                     DVGPNQPWTFELDLEKPEQWAMVIANTGPEPGTPNFHCEIAVDGAVVVSKDGAKGVLC
                     SIRLW"
     gene            complement(16069..16680)
                     /locus_tag="VF874_21655"
     CDS             complement(16069..16680)
                     /locus_tag="VF874_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9178570.1"
                     /translation="MASGRRIGAPEAKNRGVLLDAAEQLMLEEGYAAVSSRRLAEKAR
                     LKPQLVHYYFRTMEDLFLAVFRRRAEAGLRLQARALDSPQPLWALWRFGTDPTATRIT
                     MEFAALANHRKSLRAEIAYYAERFRDEQIKTVSTALSRYDIDDEVAPIVWSVLFTSVS
                     RVLVMEQELGMSAGHAETVELVERYLRHLEGEPEAISVAALEA"
     gene            16828..17520
                     /locus_tag="VF874_21660"
                     /pseudo
     CDS             16828..17520
                     /locus_tag="VF874_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            complement(17535..>18252)
                     /locus_tag="VF874_21665"
     CDS             complement(17535..>18252)
                     /locus_tag="VF874_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX9178571.1"
                     /translation="QADGDPVISVDTKKKELVGNYKAAGREWEPAGSPRPVKVHDFAD
                     KQLGKVAPYGVYDIAANTGWVNVGTDADTGQFAVESIRRWWTTMGATSYPGSDKLLIT
                     ADSGGSNGSRLRLWKTELAAFAAEAGLDITVCHLPPGTSKWNRIEHRLFSAISRNWRG
                     RPLESHEVVIETLRATTTSTGLTVNAVLDTTTYDRGIKITDKQIAALEATQLHRHEFH
                     GDWNYTLTANHTPTHPTPPT"
BASE COUNT         3171 a         6114 c         5861 g         3106 t
ORIGIN      
        1 ttggtggtcg ctgacaaccg gtacatcgcc gaggacgcga tcgagctcgt cgaggtcgac
       61 tacgaaccgc tgaccgcgat cgccgacttc accaaagccg agacctccga cgtgatggtc
      121 cacgacgcgt atccccacaa cgtcgccggc ggcctcgcgg gcgcaccgcc agacgagcag
      181 gcgttcgact cggccgcaca cgtcgtgtcc gcgaacgtct accaacagat ctatgtgccg
      241 gtgccgatcg agacgcgcgg catggtcgtc gaatggtcag cggccgccca ggaactcacc
      301 gtctgggcgt ccactcagac cccacacgag ctgcgggcgt tctgcgcgcg ctgctcggca
      361 ttcccgcaca gcgcgtccgg gtcatcatgc gcgacaccgg tggcggcttc ggccagaagg
      421 tcgtgccgat gcgggaagac atgtgcatca tgctggccgc ccgtaaggtg cccgcggcgc
      481 tgaagtggat cgaggaccgg cgcgaaaacc tgatgtctgc cgggcaggcg cgacacgtcg
      541 acggcaacgc caggatggcg ttcgacgagg acggcaagat cctggccgcc gacatcgact
      601 tcattcagga catcggcgcg tatccgacgc cgtacccggt gttgaccacc gccgcgatcg
      661 gcatgtactt ccccggcccg taccgcgtcc cgaaggccag cttcaactac aagacggtgt
      721 tctcgaacac cgccgggctc gccgcctacc gcggcccctg gcagtacgaa accctcacgc
      781 gggaaatcct tctcgacatc gcggcgcgcg agatcggctt ggatcccgtc gagttgcgcc
      841 ggcgcaatat cctgcgcggt gacgagatgc cgtacgtcaa ccccaacggc atgccgtacg
      901 accacgtcgc cccggccgac acgttcgagc aggccgtgaa gatcctcgac cacgagggat
      961 tccgcaagga acaagccgac gcgctggccg agggccgcta catcggcctc gggttctccg
     1021 cgtacatcga gccgaccgcc ccctcgatgg gctactttgc caccgagggg gcgacgattc
     1081 gcatggagcc gaccggcaag atcaacgtct atttgaacgg cggctcctgc ggcaacagca
     1141 tcgagacaac ggtcgttcag ctcaccgccg acgcgctcgg cgccggcatc gacgatgtcg
     1201 ccacaatcca gggcgacacg gcggtgacgc cgtacggggc cggcactcaa ggcagccgca
     1261 gcggtccgat gacggccggc gccgtcaacg aagcgggaac catgctgcgc aagcacatcg
     1321 tcgcgatggc agctcatcga ttgggcgtcg acgaaaccga catcgagctc ggcggatccc
     1381 gggccagtgt ccgcgaggac ccgtcgaaaa gcgtcagctt cgccgacctc gcctaccgcg
     1441 cctactacga gccgcaccac ctgccgcacg agacgtcggc atcgctggag gcgaccgctc
     1501 ggttcacatc ccgggcaccg atccactggg ccaacgcgac gcacgcgtgc acctgtgaag
     1561 tcgacgtgaa gaccggccac gtcaagctga cccgctacat cgtcagcgag gacgtcgggc
     1621 cgatgatcaa ccccaacgtg gtcgagggcc agatcgccgg cggcacagtg caaggcatcg
     1681 gcggagcgct gttggagaac ttcgtctacg acgagcacgg caacccgctg gcctcgacgt
     1741 tcgtcgacta cctgctgccc accgccaccg aagtcccgtc aattgagttc gggcacatcg
     1801 agattcccgg cccaggcgtc ggtggataca agggcgtcgg tgaaggcggt gcaatcggct
     1861 cgacgcccgc ggtgatcaat gcgatcaacg acgcgctggc gccgctcggg gtgacgctca
     1921 ctcgattgcc cgcgagtccg gcggcgatcg tcgacctgat cgaacaagca acgagataaa
     1981 ggaccaaagg tggagttagt caacgagttt cgggtaccgg tctcggtcga tcaagcctgg
     2041 gaggtgctca ccgacgtgga gcgtgtcgcg ccgtgcattc cgggtgcaca actgctgtcg
     2101 gtcaacggcg acgagttcac cggcgcggtc aaggtgaagg tcggcccgat caccgtgcag
     2161 tacaagggca aggcatcctt cgaggagaag gacgtgtcag cccgccgagc ggtgatcaca
     2221 gcccacggca aggagactcg cggtcagggc cacgccgcag cgatcgtcac tgccgaactc
     2281 aaggacgagg gggaccacac gtcctgtgtc ctcaccaccg acctctccat ctccggcaag
     2341 gccgcgcagt tcggccgcgg ggtcctcgct gacgtggccg gcaagctgat cgggcagttc
     2401 gcccagcggc tggaggccga cgtgctcgcc ggtgccaccg cctcggccac cgccgaaacg
     2461 gcggaatccc agccggtttc ggccccgacc cagccggagg acgcggcctc gatcgacctg
     2521 ctcagcttgg tggcagtgcc gctggccaag cgacatatcc cacccgtcgg gaccgggctg
     2581 gtgatcggcg tcgtgatcgg ctacctgttg ggccgccgcc ggtcatgaag tccgctccgt
     2641 tcgactatca ccgacccgac agcatcgcag ccgccacgca gatgctcgcc gaattcgggc
     2701 aggacgcaaa ggttttggcc ggtggccaga gtctggtccc gatgctggcg ctgcggctgg
     2761 cgttcttcga ccacctgatc gacatctcgc gactggacga actgaaaggc atcgagcgcc
     2821 aaaacggcgc cgtgtggatc cgtgcgggca ccaccgacgc gattgtcggt accgacggcg
     2881 agatcagcgc cgcggtgccg ctgctcacgc gagtcacccc gttcgtcggt cactttcaga
     2941 tccgcagccg cggcacgctc ggcggctcca tcgcccacgc cgaccccgcg ggcgagtatc
     3001 cggccgtcgc gttggcgctc gacgccgaga tggaagcggc ctcaccgtcg ggtcggcggc
     3061 agattcccgc cgcggaattc tttaccgggt tgtgggagac cgcgttggag ccagacgagg
     3121 ttctgaccgg tgtgcgattc ccggtgtgga cgggacggtg cggcttcgcg gtgcaggaat
     3181 tcgcccgccg ccacggcgat ttcgcgattg cgggcgcggt gatggccgtc gaactcgacg
     3241 accatgacca cgtctcccgc tgcgcgatcg gcctgctggg ccttggctct acgccgagtc
     3301 gcgcggccga agccgaagcg gcggtcgtcg gccggccggt tgccgacgtg gatgccgaca
     3361 aggtcggcgc gctggcgata accgggctcg acgacattcc cgccgacctg cagggctcgg
     3421 cgtcgtaccg cagccgggtc ggcgccgagc ttgtggcgcg ggcatggacc gaagcgatca
     3481 cggaagccag cacggaggca gtcaatgcat gagcggccgg tccgggtatc cgtcaacgga
     3541 catgacaccg aggccatcgt cgaaccccgg gtgacgctcg cggacttcct gcgtgagcga
     3601 tgcgggctca ccggcacgca tctcggctgc gagcacgggg tatgcggggc gtgcaccgtg
     3661 ctgctcgacg gtgccgccgt gcgctcctgc ctgatcttcg cggtgcaggt cgacggcatg
     3721 gaggtgacga cagtcgaagg catcgcctcg cgcgacggcg agctgtcgcc ggtgcagtca
     3781 gcgatgcgtg agtgccacgg actgcaatgc ggtttctgca caccaggttt cgtcatgtcg
     3841 atcaccgcgc tgcttcatga taatccgcat cccaccgagg aggagatccg cgaaggcctg
     3901 tccggcaact tctgccgctg caccggctac caaagcatcg tcaacgcagt gcaccgagcc
     3961 gccgaagcga cctgctagaa ctggccgcag ttgggcgtcg tcggtaatcc gttgaaccgg
     4021 tccgcgatcc actgcatcgc gcgctcgccg tccaccacca tgggcagcgc atggttgacc
     4081 accagcttgt tgaggaacgg cggctcctcg ttggtccaga actgcacgtc ggcgccttgg
     4141 gcgcaccagt cgcggcccag ctgattggcg ccggtccacg gcaccagcgg gtcgtaacgg
     4201 ttgctgacaa tcagcaccgg cgcgttgggt ttgtagcggc ctatccgctg atcgtcgaac
     4261 aggctcctga acggttcctg gtgaaccagt tcgaagatgt cttcgttgaa atacggccgc
     4321 aggtgccgaa acatgaagtt gaagatcgtc tccgcgatgc actggtcggc caccttgaag
     4381 aggaagtcct caccgcgagg cgtgagcgtc gcgcggattg ccgcctcgtg ctccggatac
     4441 atcgcgatga cggagttgag cgcgtagccg acgacaccga ccaccacgct gccgtcggcg
     4501 tacgggaaca gttctttgag gtcagccggc ggcgcgcccg cataggtccc gacgacgttc
     4561 agctccggtg cgtactgcga cgcaagttcg gcggccgacg cggcagcgcc gccgccctgc
     4621 gagtaacccc aaaatgcgac aggccatccg gttgcaacga cgtgtcgggt agctgcttgg
     4681 ccgcgcgtgc ggcgtcgagc attgcgttgc cctcggcaac ccggctgaca taggtgtgca
     4741 cgccgggcgt gccgagcccc tcatagtcgg tcatcacgat cgcgaagccg cgcgcgacca
     4801 tcgtcgagac gaacagctcc tcgtagttga acgcgaggtc ggcaaatggc gcccagtgaa
     4861 tgccctggtt gaaaagtcgc gacggtgcac actgatcgcc ctgcccctgc gtgccggggc
     4921 cgtagacgag cagtgggcgc ggtcccgtgc ccggccagtc gttgtacggc tcgaagtatg
     4981 tgccggtgac gacgttgggc ctgccgcgag cgtcggtgct gcggtacatg attcgagttc
     5041 cggtcgccat gatcgcaccc agttgtcccg acggttccag caccagccgt gacggctcgg
     5101 tgcggatcag atcgccgggc tgacccggcg gcagcggatc gggcggcgta tagaactcgg
     5161 cgtactcgtc ctcgttgaag tacggatcgt ggccgcgatc ggcgtgtgcg agcggaagac
     5221 ccgatagcgc cagcagcagg accgcgacgg ctacttcaag cagctgccgg cgtcgaccgg
     5281 aagcgtgacg ccggtgagat aacgcgcctc gtctgaggcg aagaacaaca ccgcattgct
     5341 gacgtcctcg ggttccaccc acggaatggg aagtgtatgg aacatctgac agatcggggc
     5401 catatcgtcg ggacccgggt tctccagatc gggacggaac agtttccacg ttccctcgtt
     5461 catgatcatc ggtgtgttga cgtgggtggg gtgcaccgag ttgacccgga tcatgtgctg
     5521 gcccaactcc accgcgaacg atcgcatgat gccgaccaca ccgtgcttgg cggcgacgta
     5581 gttaccgcac tgcgggtagg ccttcaggcc gccgaccgag ctggtcaaca cgatggaccc
     5641 accacggccg ccggcgatga tacggggcac accggctttc accgacttcc acacgcccga
     5701 caggttcacg tcgatcatct cttgccagtc gtgctcggtg gtcttgtcca gcgtgtcgcc
     5761 gccgttgccg atgccggcgt tggcgacgat gacgtccagc cggccgagct gctccatccc
     5821 gctgtcgacc gcagccttca gcgcgtcgta gtcgcgcacg tccacttccg ccgtcacgac
     5881 gcggcggtcg aggcccttga tcagatcggc ggtctcggcc aggtcttcgg gcgtggacgc
     5941 cggaatcgag gtgttctcga cgatcggctt gcacacgtcg acggcgatga tgtcggcgcc
     6001 ttcctgcgcc aaccgcaccg cgtggctgcg gccctggccg cgcgccgcgc cggtgacgaa
     6061 cgcgaccttg ccctcgactc gaccagccat cactacctcg cttcgatgca tgttgagcag
     6121 tcgctcagca ctctgtcatc cgcgcgcgcc gcgtgtcaat cggctttgac cggcggccgg
     6181 tagaagatca ccagctgccc gatgccgccc gcgaggccca gacccgcaga gatcgcgagg
     6241 cccgcccagc cgtccatcca gttgtccttg aagatcagcc agtccaagct gaaccggccg
     6301 gcgccaatcg tggctacggc caccgcgctc agcgcgagca ccaggttgta ctcccaacct
     6361 tccttgacga tgaagaaacc gttaggacga tgcaccgtcc acgccgcgac gagcatcagc
     6421 gcgacgaacc ccgcggccgg gatgggcgtc aacagtcccg cggccagacc gaggccggcc
     6481 gaagtctcgg tgagcgcggc gatgcgtgcg tgcagcgtgc caggcttcat cccgatgctc
     6541 tcgaaccagc gagcggtccc cggaatccgc ccgccgccga aaaacttgtt gagcccatgg
     6601 gcggccatgg tgaggcccaa aacgagccgc aagatcagca agccggtgtc gagggcgtag
     6661 tcgtgggtca tggcgaaaac ctcctggttg tcaacgggtt tcgggcgaca ccagcgccgc
     6721 cttgatgatc gcctgaacgg tgcgccggca ccgaccgcag tccgaacccg caccgcaggc
     6781 cttcgcgact tggttcgtgg tcgacgcacc gtcggcgacg gcgtcgacca ccgtctggct
     6841 cgtcacgccc ttgcacagac acacatacat cgacggcgat cccgctcaga ggttctcatc
     6901 gatgcgcatc atgtgcagga acttgccgac gaaaagcggt gggtacgcgc ccatcccggc
     6961 gcgttccatc cactccgcgg ccgcatcggg atgctcgatc caccgccgcg cgtcgtcctc
     7021 gttgtcgatc tgctgcagga tcatcacctc gcgcggatcg tcgaaagcct ggaatatcca
     7081 tgtcttctcg atgcctgcgg ccttgaagct gtcgagagcg aggtgcacgt gctcgatcaa
     7141 cgtcgtcacg tcgcccaccg acgtcatcgc ggcgacaacc acactgggcg gggtaggtgt
     7201 tgtcgagtcg acgaggtcga ggccgtcgac gagcgcgccg gcgaataccg cgggaatgtc
     7261 ttcgaccccg acggcgtcga accattcgaa gaagacccgt gagcgcagca gatcgacgat
     7321 cggctcgtgg ttgtgaacga cgatcgacac cagcacccgc ccggggtcgt tggtcgacgc
     7381 gtgcacgaag acgtgatgcg cgccgatttc ggccaaggcg gacttgcgct tctcgagaag
     7441 ccgcggcacg cgcgccggat cggggacgcg gtaatccgac gcgattacca gcgagcgcgt
     7501 gtcggccccc tcgaagttag tcactggccg gaagcggctt tgcctctttg atgctcagtg
     7561 gcgtgtcgcc gatagccagc gttccggcgc cggccttggt caccagcacc tcggccccgg
     7621 tctcgtcgac gtagcgcttc cccatcacgt ttccgccgga gaaattctgg tcgagcaccg
     7681 cgtcgctggc ggcgtcggcg ccgatctcac gcatcggaac tccaccggcg cgcaggtcgt
     7741 cgagtccgtc gccgatcttg atcacgatga cctcggtgtc acatacctgg ctcttgagcc
     7801 tggtgcccgt cttgatcatg actgtccctt cttttccagc agttcgtcaa cgatgtcgcg
     7861 ccgcaacact tttccggtcg gcgtcgtcgg caattcgtca cggaagacga ttcggtcggg
     7921 cgtgcgcgag ccccgcaggc tcttgcggac gtgcgcgcgc agtccttccg gatcgggttc
     7981 ggcgccctcg gtggggacca ccacggccac gatgatctga ccccattcgg agtcctcgac
     8041 ccccacgacc gcgacatcgc gaaccgacgg gtgctccacg agaacgtcct ccacctccgc
     8101 gggggcgatg ttctcaccgc cccggatgat ggtgtcgtcg gagcgtccgg tgatgaacag
     8161 atacccctcg tcgtcgagcg cggcgacgtc cctggtggga aaccagccgt cttcgtcgag
     8221 caccgacccg atttcggcgt acttcccgga gacctgatcg ccgcggacga acaattcgcc
     8281 gacctcaccc ggtcccagta cggcgccgtc gtcaccgcgg atctcgactt cgatgccagg
     8341 cacgggttgg ccaacggatc cgagccgcct cctgaccgtc ccgtcggtcg caccgtgtgc
     8401 cgttcggtga tcctcaggtg tcagaaccgc gatcgtggaa ctggtttcgg tcaacccgta
     8461 agcgttcacg aagcccaccc cgggcagcag gtcaagggcc ttgcgcacca gcggcagagc
     8521 gactttggag ccgccgtagg ccagcgtaca aagggtcggc agcggcgtgg ggtgcttctc
     8581 cagctcgctg acgatccgat ccagcatggt gggcaccacg gtagccgccg tgacaccttc
     8641 gtcgcggacc agccggatcc actccttggg gtcaaaccgg gtcaggtaca ccatctttcg
     8701 gcccgcatac acgttggaaa gcgcggcgcc cacaccggcg atgtggtacg gcggcacgca
     8761 gatcaacgcg gcatcgccag ggtctgccga agcgaattcg accgttccca tcacgtagct
     8821 cgtcaagttg ttgtgcgaca actcgactgc cttgggccgg gacgtcgtgc cggacgtgaa
     8881 cagcatgatc gcgacatcat cgggatcagc gaactccgcg gcgggctccg ccgtgcgcgc
     8941 catcgtgacg aaatcctcgc agctgaacga ctttacgcca gcgccctcca ccatgtcgcg
     9001 gtagtcgtcg tcgaacacca ccaacggttc cggcagccgt gcgatcagct catgcagccc
     9061 ctccgcgctc agccggtagt tcagcggcgt gaacggccga gcggcccggg ccgacgagaa
     9121 gatcagcaac ggcagcatcg ctccgccggt cccgacgtag gcgatgctgc cggcgccgga
     9181 ggccgcgatg acacccgcac ctccgtcggc cagagcgctc aactcaccca cggtcaggcg
     9241 ggtcccgtcg gagaccaacg ctccccggtc gggatcgccc gacgacgcca tctcgagcag
     9301 caacgaaatg gtcatggcag cctcagaccg ggttgaagga cgaaatcagc gacttgccat
     9361 cgactttcgt cagtttgacc atcacgctgc tggcagcctg cgacggcgcc gggtcgccgg
     9421 cggccatcgt ggtctggttg atgtagagaa ggacgtccgc cgaatcgggg cgcaattccg
     9481 acacggcgtt gcgcacgacg gtagccgacg tgttgatcga cttctgcttg gcgacaggcg
     9541 cgaccgcagt ttgaatgtac tggtcgtact gcgccaagaa gtcgccggtc agatgtgacc
     9601 gggcggacga tacgtcgcgg tcgaagttgc ccgaggagta cgtcagcacc gcgaccgctc
     9661 cttcggtcgc cgcatcgatc gcggactgct tcgtcgaatc gttcatctcc cggtcggggc
     9721 ggtactgcgt atacaggagc accgccagca gcgaaaccga cgccacgaca agcacaatcc
     9781 acaccgcggg aacgatattg ccgacgagcc tcctcttcgg cgcggcctca gggacgtcga
     9841 tgtcctcgac cggcggcgct tcgtctgcgg tcacggcacc aactcgactt tcgctagctt
     9901 gaaccggtca ccatcccggg tcaccgtcac gctgagccgt aacacccgcg gatccttctt
     9961 cgcgccggcc gagttggtga tctgctcttc ggccaccacc aggacgacgg ccgtgtcgtc
    10021 ggacatcgac tgcaccgcag tcgctttgac cgtgccttgg gcgaccacct tcgacgtttc
    10081 caccactttg acgaaatcgt cggccctggc ttggaagtcg tcgcggaagg agccggtgga
    10141 gttgtcgatg atgcgctgca cgtcgtccct ggcgtggttg aagttcagtg aggtcagcgc
    10201 gacgatgcct tctttagcgg ccgcggcgaa ctcctcggaa cgctcgcgct gcgccgattt
    10261 ggtgtggtgc tgccagagga tgactccact gcccgccagg gaaccgacga tgacgagcgc
    10321 ggcgattgcc ttcgcgaccg tgctccatcg cggccggcga cggtaccacg gtcgctgcgc
    10381 ttgctcggcc tcgccgtccg tctcggcctc gccgtccagc cggcccgacc ggatcgcccg
    10441 cacgcgcgca cgcgcggccg ccgcggcggc ttcggcctcg gcggcctcgg cctcggcctg
    10501 ttcgatctcc gcctgcaggt cagtgctcga ctcctcggtg ggtgagtcgg gctccgtgtc
    10561 cgacgcactg acgtcggtgg tcactgactc accgctttga gcagacggct ttttggtgtc
    10621 gcccatgaca acttcatacc gaactccggt gctgctggtc gttgaccggg gaattctcca
    10681 ccgacccgca accacgacgg tgtccggtcg gcatctgggc tcctgggtgt cggcggtttc
    10741 ttggagaact acgttatcat ttgccgccca acggttacca agccagtctc ctttgcggcc
    10801 cccaaaaatt acctttcatc tgcgggaaca tcatttccga attgagcaag tgccgcttcc
    10861 gtcccgtcgc acccgcgatc ccggccaccg tcgccgccgc cgtcggcgac aaccccgaac
    10921 cccgaaaccc cacctgacgc cgaacgtgcg gccactgcga aaaaccgaaa tctcgcagtg
    10981 agctcacgtt cgacgaagtg agcatctcag ggcgcagacc cggctaccgc agtcggtctt
    11041 tctgcacctt gccagtggcg ttgaggggca acgcgtcgag gaactccacg aaccgcggca
    11101 ccttgaaccc cgccatccgg tcgcgcgacc agctgatcag ttcgtcgccg gtcacctcgc
    11161 ccttcgccac cacgaacgcc ttgccgacct ggcccagccg ctcgtccgga acaccgatca
    11221 ccgccgcctg ctcgacggcg ggatgctcga ggaggaagcc ttcgatctcg gcggggtacg
    11281 cgttgaaccc gccgacgatg aacatgtctt tcttgcgtcc cacgatgcgc agccgtcccg
    11341 agccgtcgaa cttcccgagg tcacccgtgt gcagccagcc ctggtcgtcg atcgcgtcgg
    11401 cggtggcgac ggggtcatcg aaatatccct tcatcacgcc gtacccgcgg accagtacct
    11461 cgccgtcgtc ggcgatccgc acgtcgacgc cgtcacaagg aagaccggcg gtggtcgcga
    11521 catgatcgaa ggaatcgccg ggacgcgaca gcgtcacatt gccggcctcg gtgaggccgt
    11581 accccgtcat gagtgtctgg aacggaagtt cctcgtgcac gcgccgaacc agctcgaccg
    11641 ggatgtcggc ggcgccggtc acccccgcgc gcaacgacga cagcctgctc ttgtcgccga
    11701 cagtgagcag cgagtgatac agcgtcggcg gacccggcag catcgtgatc tgttcggact
    11761 caatcagttc gacgacccga tcgatgtcga agacggcaac gggcagcatc gtcgcgccgc
    11821 gcaggaagga cgcgatcagc ccagctttgt agccgaacgt gtggaagtac gggttgacca
    11881 tcaggtagcg gtcaccctcg cgcagatccg caagagtggc ccattcctca tacatccgca
    11941 gcgtctgaag atgatgcatc atcgcgccct tgggccggcc cgtcgtaccc gaggtgtaga
    12001 tgatgtctgc ggtgtctgtg cctttcaggc cgttcacctc gtgttcgaac ggttggccgc
    12061 taccgaggaa gtcggacttc aggtcgatcg ccgggacgcc ggcgggaacg tcgaattcct
    12121 ggcccaggaa accggcaaag aagcccgtgt cgacgagcac ggccttcgcc ccgctgcgac
    12181 ggatgatgtc gccggcttcc tcggtcttga accgggtgtt gaccggtacc agcacaccgc
    12241 cggccgtcag gagaccgaac gcggcgatga tccactcggc ggagttgggc gcccacaccg
    12301 ccacccggtc gcccttctgg acgccgagag ccgcaaacgc tcccgcggcc cggcgaatcc
    12361 gttcggtcag ctcggcgaac gacagccgca gtggcccgtc gaccactgct tccgcgtcac
    12421 caaaccggtc gcccgcggac aggaccatct cggggatggt ctgccacggt cgagggtccg
    12481 tcacaccgtg acgagccggg acaggttgcc ccccatgatc ttcgcctggt cgtcgacgga
    12541 gagatgctcg agcgcagtca catagtgcgt cggctccgca agcccttccg gatgcgggta
    12601 gtcggagccg tagagcacgc ggtccacgcc gatgaggttg atcaggtcgt cgatgccctc
    12661 ctcgtagaac gggctgacgt ggatgcggtt cttgatctcc tcgatcgggt tgccggggaa
    12721 ggcctcgggg gccttcttgt acacctcggc cattgaatcc agcagcggga acatccactt
    12781 cgacccggcc tcgatgacgg cgaccttgag cgtcgggaac cggaacagcg cgccgtgaat
    12841 cacccacgag gccaccgcat cctgaaccgg gcgccactcg ttgagaatgg ccatcgcgtt
    12901 ggtctggaac ggcaacatct cctgggcggc gccgtcccac tcggaggtgt agcgggagta
    12961 gccgctgtcc gaggagtgca tggcgaccag cacgtcgtgt tcgacgcact tctgccagaa
    13021 cggatcgaac tcgggcaggg cgaacgaccg cggcccgcgg aaaccgggca ccggagcggg
    13081 gcggatcagg atgacccggg caccgcgctt gaccacccag tccagctcct cgatggcctt
    13141 ctcgacgatg ggcagggtga tcaccggagt ggtgaagatg cggttctggt aattgaaccc
    13201 ccagacgtca tcgagccact cgttcagcgc atgcacgatg acgtgaatcg cgaccgggtc
    13261 gtcgcgcagc cgctcctcga ccagactggc aagggtcggg aacatcagcg agcggtccag
    13321 cccgagttcg ttcatgagct cgagccgcgg accgggctcg aagaacgccg gaatcgaccg
    13381 catcggctcg ccgaagagct cgcgcttgct cttgccgtcg ggattgccgt acttgaagta
    13441 ctcctcccac gcgcccggtt tggcgaccac cgagaaggtg gggttcggga tgtagttgct
    13501 gatctgaccg cgcagcgcga tcttggtgcg gccgttgacc tgcacgtact ggacgacgtc
    13561 cttgtactcc tcaggcagga acttggtcag cgactcctcg gtctcataca gatgattgtc
    13621 cgcatcgaac agcggaaacg gtatgtcgac cctatgcgaa agttgaccca cggaacactc
    13681 cttttccttt tgggagactc ctattctcat cttggttggg tggcaatccc ctgccggact
    13741 gcggacttct ggatcttgcc gctcgcggtg cgcgggtaat cctcgacttc gtgcagctcc
    13801 tccggccatt tctgggtggc cacgcctatg cgccggaagt gctcccggac ctcgtcgagc
    13861 gtcggcatca cgtgacccgc ccggacccgc agcacggccg cggcgtgctc gcccagccgc
    13921 tcgtcggcag cgcccaccac caccgcctcc gcgacggccg gcatcgcgag aagcacctcc
    13981 tcgacttcga ccgcgctgat gttctccccg ccgcggatga tcacgtcgga cttgcggtcg
    14041 gtgatcgtca agtagccgtc gtcgtccagc acgccgacgt cgccggtccg gtaccagccc
    14101 tcgtcgtcga atgcggcccg tgtcatggct tcgtcgatgt aaccgaggca caggtccggg
    14161 ccccggctga agatctcgcc gtcgtctccc agccggatct ccacgcccgg acgcgggcac
    14221 ccgtccgtca acaaccgctt gtcctccggc ccgtcgtgcg gcgttccggt gaccgacgga
    14281 tgctcggtgc tgccatacga gcggaagacg tggatgccca ggtcggtgag ccgacgggtg
    14341 accgcggccg ggaccgtgga tccgccgagc ccggcggtct tcatgtgctg cagatgctcg
    14401 tcggtgaagt cgggatgatc cagcaagctc gtgaagaagt actgcgggcc accaccgatg
    14461 gccaggcctt cgcgtttcat cagcgtcagc acctcggtcg ggtcccaatg gtcgacgagg
    14521 tcgatcgacg agccgtccat caccgggatc aggaaagccc cgagcatccc gatgaaatgc
    14581 ccgaccggag cggccgtgag ctgccgaccg cggttgggtg ggtagttcgc gtgcaactgc
    14641 ccacgccccg cgcccgcaat ccggcggcga gccggcgggc caccagttcg acgtcgcgat
    14701 aggtgcccga gaacggacgc acgttcgaat gcacacagaa cgcggcgtcg ggccaggctt
    14761 ccagaccgcg ggccagcagg tcgccgaggg tgtcctgcgt ccaccaaccc tcgtcctcgt
    14821 agcgcttggc caattcaacg ggaatgggtc gcgtcggaac gcttcgaccg gcgataagca
    14881 tgacgatgga gatgctattc tccatcaaag agaatgccaa tatcgcatcg gagaacgtcc
    14941 agatggtgga cttggagctc gacgacgggc tggcgacgct caccatcgac cgcccgcacg
    15001 cccgcaatgc catagcgctg gacacgatgg ccgagctgga gaaggtgctc gacgctctcg
    15061 actcctccag cggcgctcgg gccctggtga tcaagggcgc cggcgacaag gccttcgtct
    15121 cgggcggcga cctcaaagaa ctcgccgcaa tcagaaccac cgaagaagcg tccgcgatgg
    15181 cgtggcggat gcgcacgatc tgcgaccggc tcgcgcagtt ccccgcgccg gtgatcgccg
    15241 cgctcaacgg tcatgcgttc ggcggtggcg ccgaggtggc cgtggccgcc gacatccggg
    15301 tcgcggccga cgacatcaag atcgccttca accaggtgtc cctggagatc atgcccgcat
    15361 ggggaggcgc cgagcggctg gcggcgctgg tcggcaaggg caaggccctg atgttggcag
    15421 ggacagggac cgtgttggat gcggccgagg cggagcgcat cgggctggtg gaccgggtag
    15481 tgacccgagc gtcgttcgac gagagctggc aggcgctcgc gcggtcgctg gccaaccgcc
    15541 cggcgggcga gataaaacgc gtaatcgccg gggtgagccc cgacgaagcg gtagcctact
    15601 ttgcgcggct gtgggtcggg gacgaacatt ggcaggccgc ggaccgagtg atgaggcgtc
    15661 gatgagcctc ttgggcgaag agtgaaggca cagcaaatga tgacgaccag aacgatcctt
    15721 gccgcggggt cgaagccctt ccgctgcatc acgtgaggta cacgctcacc gccgagaacc
    15781 cgatctgggc ggagatctac tacctcgacc acgagccggc cgtcttcgcc gactggagtc
    15841 acaaacccta cgagttcatg ccgaacgtcg aagcggacgt cggcccgaac cagccgtgga
    15901 catttgagct cgacctggag aaaccagagc agtgggccat ggtgatcgcg aacaccggac
    15961 ccgagccggg gacgccgaac ttccactgcg agatcgcggt ggacggggcc gtcgtcgtat
    16021 cgaaggacgg cgccaaaggg gtgctgtgct cgatccgtct gtggtgagtc aggcctctag
    16081 ggctgcaacg gaaatggcct cgggttcgcc ctcgaggtgc cgcaggtaac gctcgacgag
    16141 ttcgacggtt tcggcgtggc cggcagacat acccagctcc tgttccatca ccagtacccg
    16201 cgacacgctg gtgaacagca cagaccacac gataggcgcc acctcgtcgt caatgtcgta
    16261 gcgcgacaaa gcggttgaca ctgtcttgat ctgctcgtcg cggaaacgtt cggcgtagta
    16321 ggcgatttcg gcccgcaatg acttgcggtg gttcgccagc gcggcgaact ccatggtgat
    16381 ccgggtggcg gtcgggtcag tgccgaacct ccatagcgcc cacagcggct gcggcgaatc
    16441 cagcgcccga gcctgcagcc tgagtcccgc ttcggcgcgc cgccggaaca ccgccaagaa
    16501 caggtcttcc atggtgcgga agtagtagtg cacgagttgt ggcttcagcc gcgccttttc
    16561 ggcgaggcga cgggaactca ctgcggcata gccttcttcg agcatcaatt gctcggcagc
    16621 atcgagcagc acgccgcgat tcttcgcctc cggcgcccct attctccggc ccgatgccat
    16681 gccctcaccc tgcgttgtcg accttgtcgg atcaaatgcg cacatcgtaa ccctgatctt
    16741 gaccacgacc caccaccatg ctaagcagat gcacagcata cgtcgcttgc cacagtccgg
    16801 cgagtgctcc aggaggccgg ccaccccatg acagacttcg acacagtcga ctacttcacg
    16861 gatcagtcgc tcattccgga tccgcatccc tatttcgacc acctgcgctc ccagaacccg
    16921 gtgtgttgtc cgatcaacaa cggcgtgctg gccgtgaccg gctgggaggc ggcgaacgcg
    16981 gtctacaagg acgtcgactc ctactcctcc tgcgtcgctg tcgccggccc gttcaccccg
    17041 ctgccgttcg tgcccgaagg tgacgacatc tgcgcgctga tcgagcagca ccgcaccgag
    17101 atccccttgt acgagcacat ggtcacgatg gatccgccgg accacaccaa cgcacgctcg
    17161 ctgctgagcc gtctgctgac gccgaaacgg ctcaaggaga acgaggactt catgtggcgg
    17221 ctggccgacc gccacatcga cgagttcata gccgacggcc aatgcgaatt cctggccgcc
    17281 tacgcgcggc ctttctcgct gctcgtcgtc gccgacctgc tcggcgtccc cgaacaggac
    17341 cacgaaaagt tccgctccaa cttcggcgcc gaccggccgg gcacaaatat cggcggactc
    17401 gaccacgagc ccatcgcgac gaacccattg gagtgggccg acgagatgtt cagcttctac
    17461 atcgaggatc gccggcgtaa tccccaagac gacgtgctga ccggcctagc cacggctaag
    17521 ggagcgcgca gtaattaggt aggtggcgtc gggtgtgtcg gggtgtggtt ggccgtgagc
    17581 gtgtagttcc agtcgccgtg gaactcgtga cggtgcagct gggtggcttc gagggcggcg
    17641 atctgcttgt cggtgatctt gatgccgcgg tcgtaggtgg tggtgtcgag caccgcgttc
    17701 acggtcaatc cggtggatgt ggttgttgcc ctgagagttt cgatgacaac ctcgtgggat
    17761 tccaggggtc gtccgcgcca gttgcggctg atcgcggaga acagccggtg ttcgattctg
    17821 ttccatttgg aggtgccggg cggcaggtgg cagaccgtga tgtcgagtcc ggcctcggcg
    17881 gcgaaggcgg cgagttcggt tttccacagc cgcagccggg atccgttgga gccaccggag
    17941 tcggcggtga tcagcagctt gtccgatccg ggatagctgg tagcgcccat cgtggtccac
    18001 cagcggcgga tcgattccac cgcgaactgg ccggtgtcgg cgtcggtgcc gacgttgacc
    18061 cagccggtgt tggcggcgat gtcgtagacc ccgtagggag ccaccttgcc cagctgcttg
    18121 tcggcgaagt cgtgcacctt caccgggcgc ggtgacccgg cgggctccca ttcacggcca
    18181 gcagctttgt agttgccgac gagttccttc ttcttggtgt ccacgctgat caccgggtcc
    18241 ccgtcggcct gg
//
LOCUS       DASWBF010000580         8457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_919859,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000580 DASWBF010000000
VERSION     DASWBF010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919859"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..2881
                     /gene="polA"
                     /locus_tag="VF874_21670"
     CDS             218..2881
                     /gene="polA"
                     /locus_tag="VF874_21670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX9178572.1"
                     /translation="MLLDGNSLAFRAFYALPAENFKTQGGLTTNAVYGFTAMLINLLR
                     DEAPTYVAAAFDVSRQTFRSDRYPEYKANRTSTPDEFRGQIEITKEVLFALGITVLAE
                     PGYEADDVIATLATQAEEVGFRVLVVTGDRDSLQLVSDDVTVLYPRKGVSELTRFTPE
                     AVVDKYGLTPQQYPDFAALRGDPSDNLPGIPGVGEKTATKWIAEFGSLQALVDNVDSV
                     RGKVGDALRANLSHVILNRELTDLVKDVPLAQTPDMLRMQPWDRDHIHRLFDDLEFRV
                     LRDRLFDTLAAVEPEVDEGFDVRGGALAAGEVADWLATHAADGRRAGVSVVGTHAVAD
                     GDATAVAIAAADGDSGYIDTATMTSEDETALGRWLADEIQPKALHEAKWSMHDLAARG
                     WELRGVTSDTALAAYLVRPGQRSFTLEDLSLRYLRRELRADNPEQVQLSLLDDSDGVD
                     DQAVQTQILRARAVVDLADALDAELDRMECSSLLGDMELPVQRVLANMEKAGIAVDLA
                     QLTDLRHQFAEQIRDAAEAAYAVIGKQINLGSPKQLQVVLFDELEMPKTKRTKTGYTT
                     DADALQSLYEKTGHPFLQHLLAHRDVTRLKVTVDGLLNSVATDGRIHTTFNQTIAATG
                     RLSSTEPNLQNIPIRTDAGRLIRDAFVVGGGYAELMTADYSQIEMRIMAHLSKDEGVL
                     EAFNTGEDLHSFVASRAFGVPIDEVIPELRRRVKAMSYGLAYGLSAYGLAQQLKISTE
                     EAKDQMDQYFARFGGVRDYLHEVVNQARKDGYTSTVLGRRRYLPELDSSNRQVREAAE
                     RAALNAPIQGSAADIIKVAMIQVDNALTTAGLKSRMLLQVHDELLFEVASGERDELEA
                     LVREKMGSAYPLDVPLEVSVGYGRSWDSAAH"
     gene            complement(2878..4056)
                     /locus_tag="VF874_21675"
     CDS             complement(2878..4056)
                     /locus_tag="VF874_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX9178573.1"
                     /translation="MSYRGTPQPRPGGRPLHPPFHPPFQRRIRKVGAPLGVIITLGTV
                     AGLIVILMTAVNPVGTAIGFLLSSVAMAVVVLAYLWLDRWEPEPPRLLVLAFVWGTSV
                     AVVVSVALEVYFDSLINRGETTVSFASVAIGAPMIEEAAKGLFLLIMMTGRRRNELNS
                     LTDCLVYAGITAAGFAWLEDILYISGGGSLAGSLVTAAMRLIMGPFAHPLFTTMIGVG
                     VYFALQQRNALAKGGCILLGYLGAVIMHGLWNGSSLLSLEAYFLVYLLWMVPIFGLAI
                     WLGVASRRREQWVVGAKLPGMVAAGLLTPNEATWLGSIRNRKLAIGEATRFGGQPAGK
                     AVKNFAAQVVELAFVRDRIERGFGDERVFALQTEEAYGVMAARAGAPILQRIAGYRAP
                     "
     gene            4201..5646
                     /gene="rpsA"
                     /locus_tag="VF874_21680"
     CDS             4201..5646
                     /gene="rpsA"
                     /locus_tag="VF874_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX9178574.1"
                     /translation="MPSPSVTSPQVAVNDIGSSEDFLAAIDKTIKYFNDGDIVEGTIV
                     KVDRDEVLLDIGYKTEGVIPSRELSIRHDVDPNEVVSVGDEVEALVLTKEDKEGRLIL
                     SKKRAQYERAWGTIEELKEKDEAVKGTVIEVVKGGLILDIGLRGFLPASLVEMRRVRD
                     LQPYIGTEIEAKIIELDKNRNNVVLSRRAWLEQTQSEVRSEFLNQLQKGSIRKGVVSS
                     IVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVQVGDEVTVEVLDVDMDRERVSLSL
                     KATQEDPWRHFARTHAIGQIVPGKVTKLVPFGAFVRVEEGIEGLVHISELAERHVEVP
                     DQVVQVGDDAMVKVIDIDLERRRISLSLKQANEDYTEEFDPSKYGMADSYDEQGNYIF
                     PEGFDSETNEWLEGFDQQRTEWEARYAEAERRHKMHTTQMEKFAAAEAAAGEKQPAAN
                     GAPRSEEAQSGGSLASDEQLAALREKLAGSA"
     gene            5717..6877
                     /gene="coaE"
                     /locus_tag="VF874_21685"
     CDS             5717..6877
                     /gene="coaE"
                     /locus_tag="VF874_21685"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX9178575.1"
                     /translation="MLRIGLTGGIGAGKSTVSATFAECGGIVVDGDVIAREVVQPGTE
                     GLDKLVDAFGDDILLPDGALNRPALAAKAFGDEDKRATLNGIVHPLVARRRAEIISAV
                     SEDEVVVEDIPLLVESGMAPLFPLVVIVHADPEVRVERLIKRGMDEADARARIAAQAS
                     DEQRRKVADVWLDNSGSEGELVERTRGLWHQRILPFAHNVSTRQTVRAAQQLVPYDPT
                     WPAQAHRIIERLKTACGHHALRVDHIGSTAISGMDAKNVIDVQITVASLAVADELADT
                     LADIGYPRIEGITSDVPHSDQPSLWRKRIHASADPGRPAHIHVRVDSWPNQQFALLFT
                     DWLRANPGVREDYLAAKRRAVATPEYTEAKEPWFLDNYRRAWEWADSTGWSP"
     gene            complement(6880..7413)
                     /locus_tag="VF874_21690"
     CDS             complement(6880..7413)
                     /locus_tag="VF874_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178576.1"
                     /translation="MSVTIRACLTTTLLAALVAGGQFLPSTALADEQLPQEMHNVTYR
                     ARIDGVSRGLTITYNVSDTAVETANPTVLPGQTFEANAVMADPAHAGMEISIRWPYTA
                     NLHCEILVDDALTAQADDFIAPRFLPQSNDPGYGVMACGGPLANAAAVDAGEAVNTTA
                     VPEPAPEASPSEVAPPN"
     gene            complement(7425..7931)
                     /locus_tag="VF874_21695"
     CDS             complement(7425..7931)
                     /locus_tag="VF874_21695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEX9178577.1"
                     /translation="MHPPKRETFDLVARTNTDPKGIVRAVDVYTVKPWGLYMARPTPG
                     RAQFHYLESWLLPSLGLRVNVFHFNPGHEREQDFYLDIGEIIPGETEWRAEDHYLDLV
                     TYANERTDLLDVDELLDAHHHGLVSSQTAEQAIQRAVHVIEGLSENGHCVHRWLSGHG
                     MHLTWAGA"
     gene            7998..>8457
                     /locus_tag="VF874_21700"
     CDS             7998..>8457
                     /locus_tag="VF874_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX9178578.1"
                     /translation="MAFATEHPVLAHSEYRAVEEAVRTGGRFEVVSQHQPAGDQPAAI
                     DELERRIKSGERDIVLLGATGTGKSATTAWLIERLQRPTLVMAPNKTLAAQLANELRE
                     MLPHNAVEYFVSYYDYYQPEAYIAQTDTYIEKDSSINDDVERLRHSATSSL"
BASE COUNT         1591 a         2756 c         2802 g         1308 t
ORIGIN      
        1 cctcgggtgg cctctgctga acaagcagca ctcggcctac ccccgaacag cccaggcaca
       61 cctggacgtc gcggacccag ccgaaaacaa ccagtgtcca agctgattgg cgctgcatga
      121 cccactacga gtcaactctt agagtgagtc cgtgacccca gccaagaccg tgcccgctca
      181 agccgcactt ccggaggcgg ccgacaagcc catactgatg ctgctggacg gcaattcact
      241 tgcctttcgg gcgttctacg cgctgccggc cgagaacttc aagacccagg gcggtctgac
      301 caccaacgcg gtgtacgggt tcaccgcaat gctgatcaac ctgctgcgcg acgaggcacc
      361 cacgtatgtt gcggccgcgt tcgacgtgtc tcgccagacg ttccgctccg acaggtaccc
      421 cgaatacaag gccaaccgca cgtccacccc cgacgagttc cgcggccaga tcgagatcac
      481 caaggaggtg ctgttcgcgc tcggtatcac ggtgctcgcg gagccgggat acgaagccga
      541 cgacgtcatc gcgacgttgg ccacgcaggc cgaggaggtc ggcttccgcg tcctcgtcgt
      601 caccggcgac cgcgactcgc tgcagttggt cagcgacgac gtgaccgtgc tctatccaag
      661 gaaaggtgtc agcgagctga cccgcttcac cccggaggca gtggtcgaca agtacggact
      721 gactccgcag cagtatccgg acttcgccgc actgcgcggc gatcccagcg acaacctgcc
      781 cggcatcccg ggtgtggggg agaagacggc aaccaagtgg atcgccgaat tcggctcgct
      841 tcaggcgctc gtcgacaacg tcgactcggt gcgcggcaaa gtcggagatg cgttgcgcgc
      901 caatttatct catgtcatac tgaaccgcga gctgaccgat ctggtgaaag atgtcccgct
      961 cgcgcagacc cccgacatgc tgcgcatgca gccctgggac cgcgaccaca tccaccggct
     1021 gttcgacgac ctcgaattcc gcgtgctgcg ggaccggttg ttcgacacgc tcgcggcggt
     1081 ggagccggag gtcgacgagg gattcgacgt ccgcggaggc gcactggcgg ccggtgaggt
     1141 ggcggactgg ctggccacac atgccgccga cggacgcagg gcgggcgtgt cggtggtcgg
     1201 cacgcacgcg gtagccgacg gcgacgccac cgcggtcgcc atcgccgccg ccgacggcga
     1261 ctccggctac atcgacaccg cgacgatgac gtccgaggac gagacggcac tcggccgctg
     1321 gctggccgac gaaatccagc cgaaggccct gcacgaggcg aaatggtcga tgcacgacct
     1381 ggcagcccgc gggtgggagc tgcggggtgt cacctccgac accgcgcttg ccgcgtacct
     1441 ggtgcgtccg ggccagcgca gcttcacgct ggaggacctg tcgctgcgct acctgcgtcg
     1501 cgagctgcgc gccgacaatc ccgagcaggt tcagctgtcg ctgctggacg acagcgacgg
     1561 cgtcgacgac caggccgtgc aaacccagat cctgcgggcc agggcggtcg tcgaccttgc
     1621 cgacgcgttg gacgccgagc tggaccggat ggaatgctcg tcgctgctcg gcgacatgga
     1681 gctgccggtg cagcgcgtgc tcgccaacat ggaaaaggca ggcatcgcag tcgatctcgc
     1741 gcaactgacc gacttgcggc accagttcgc cgagcagatc cgcgacgccg ccgaggcggc
     1801 gtacgcggtg atcggcaagc agatcaacct gggttcgcca aaacagttgc aggtggtgct
     1861 gttcgacgag ctcgagatgc cgaagaccaa gcgcaccaag accggctaca ccaccgacgc
     1921 cgacgcattg cagtcgctgt acgagaagac cggccacccc ttcctgcagc atctgctggc
     1981 ccaccgcgac gtcacgcggc tcaaggtcac cgtcgacggg ctgctcaact cggtggccac
     2041 cgacggccgg atccacacca cgttcaacca gacgattgcg gcgaccgggc ggctgtcgtc
     2101 caccgagccc aatctccaga acatcccgat ccgcaccgac gcgggccggt tgatccgtga
     2161 cgcgttcgtt gtcggtgggg gttacgccga gctgatgacc gccgactaca gccagatcga
     2221 gatgcggatc atggcgcacc tgtcgaaaga tgaaggcgtg ctcgaagctt tcaataccgg
     2281 tgaagacctg cactcgttcg tcgcctcgcg ggcgttcggc gtcccgatcg acgaggtcat
     2341 cccggaactg cggcgccggg tcaaggcgat gtcatacggg ctggcctacg gactcagcgc
     2401 ctacgggctc gcccagcagc tcaagatctc cactgaagaa gcgaaggacc agatggacca
     2461 gtacttcgcc cgcttcggcg gagtccggga ctacctgcat gaagtcgtca accaggcccg
     2521 gaaggacgga tacacgtcga cagtgctggg ccggcgtcgc tacctgcccg agctggacag
     2581 cagcaaccgc caggtccgtg aagcggccga gcgcgccgcg ctcaacgccc cgattcaggg
     2641 cagtgccgcc gacatcatca aggtcgcgat gatccaggtc gacaacgcgt tgacgactgc
     2701 cgggctgaag tcccggatgt tgctgcaggt gcacgacgaa ctgttgttcg aggtcgcgtc
     2761 gggtgagcgt gacgagctcg aggccttggt ccgcgagaag atgggcagcg cgtacccgct
     2821 ggatgtaccg ctggaagtct cggtcggcta cggccgcagc tgggattcgg cagcgcacta
     2881 aggcgcgcgg tacccggcaa tccgctgcaa aatcggcgca cccgctcgcg ccgccatgac
     2941 tccgtacgcc tcctcggtct gcagagcgaa cacccgctcg tcgccgaagc cgcgttcgat
     3001 ccggtcgcgc acgaaggcca gctccaccac ctgggcggcg aagttcttga cagctttgcc
     3061 ggcgggctgc cccccgaacc gcgtcgcctc gccgatcgcg agcttgcggt tgcggatcga
     3121 gcccagccag gtcgcctcgt tgggggtcag caggccggcc gccaccatgc cgggcagctt
     3181 ggcgccaacc acccactgct ccctgcggcg gctcgcgacg ccaagccaga tcgccagccc
     3241 gaagatcggc accatccaca acagatagac gaggaaatag gcctccaggc tcagcagcga
     3301 cgacccgttc cacagcccgt gcatgatcac cgcgcccagg tagccgagca ggatgcagcc
     3361 gcccttggcg agcgcatttc gttgctgcag agcgaagtac accccgacgc cgatcatcgt
     3421 ggtgaacagc gggtgggcga acgggcccat aatcagccgc atcgccgcag tgaccagcga
     3481 gccggcgagc gaccctccgc cggagatgta gagaatgtcc tccagccagg cgaaacccgc
     3541 cgcggtgatc cccgcataca ccaggcagtc ggtcagcgag ttgagctcgt tgcggcgacg
     3601 gccggtcatc atgatcagca gaaacaggcc cttggccgcc tcctcgatca tcggcgcgcc
     3661 gatcgcgacg gaggcaaagc tgaccgtcgt ctcgccgcgg ttgatcaagg agtcgaaata
     3721 aacctccagc gccaccgaaa cgacgacggc gacagatgtt ccccacacga acgcgagtac
     3781 gagcagcctc ggcggttccg gctcccatct gtccaaccac aggtaggcga gcacgacgac
     3841 cgccatagcg acactggaga ggaggaaccc aatcgccgtg ccgaccgggt tgaccgcggt
     3901 catcaggatc acgatcaacc cggcgacggt gccgagcgtg atgatcacgc cgagcggcgc
     3961 cccgaccttg cggattcgcc gctgaaacgg tggatgaaag ggcggatgca acggccgacc
     4021 gcccggacgc ggttgcggcg tgccccggta tgacatcaca gcagggtagc cgccttctgg
     4081 accaagtttg tccagcatgt acacggtcga gtagcctcga cgggtgagtc gcgaccagat
     4141 cgcgggtgtc accatgccac cagtccctac gacctaacct gtccggagca acccaacaac
     4201 atgccaagtc cctccgtcac ctcgccgcaa gtagccgtca acgacatcgg ctcgagcgag
     4261 gactttctcg ccgccatcga caaaacaatc aaatacttca acgatggcga catcgtggaa
     4321 gggacgatcg tcaaggttga ccgtgacgaa gtcctgctcg acatcggcta caagaccgaa
     4381 ggtgtcatcc cgtcccgcga gctctcgatc aggcacgacg tcgaccccaa tgaagtggtg
     4441 tccgtcggcg acgaggtcga ggcgctcgtt ctcaccaaag aggacaaaga gggtcggctg
     4501 atcctgtcca agaagcgcgc ccagtacgag cgcgcctggg gcacgatcga ggagctcaag
     4561 gagaaggacg aggccgtcaa gggcaccgtc atcgaggtcg tgaagggtgg cctgatcctc
     4621 gacatcgggc tgcgcggctt cctgcccgcc tcactcgtcg agatgcgtcg cgtgcgtgac
     4681 ctgcagccgt acatcggcac ggagatcgag gccaagatca tcgagctgga taagaaccgc
     4741 aacaacgtcg tgctgtcccg ccgtgcctgg ctggagcaga cgcagtccga ggtgcgcagc
     4801 gagttcctca accagctgca gaagggcagt atccgcaagg gcgtggtctc gtccatcgtc
     4861 aacttcggcg cgttcgtcga cctcggcggt gtggacggtc tggtgcacgt ctccgagctg
     4921 tcctggaagc acatcgacca cccgtccgag gtcgtgcagg tcggcgacga ggtgacggtc
     4981 gaggtgctcg acgtcgacat ggaccgtgag cgggtttcct tgtcgctcaa ggcaactcag
     5041 gaagatccat ggcgtcactt cgcccgcacg cacgcgatcg ggcagatcgt gccgggcaag
     5101 gtcaccaagc tggtgccgtt cggtgcgttc gtccgcgtcg aggagggcat cgagggtctc
     5161 gtgcacatct ccgagctggc cgagcgccac gtcgaggtgc cggaccaggt ggtgcaggtc
     5221 ggcgatgatg cgatggtcaa ggtcatcgac atcgacctgg agcggcgccg catctcgctg
     5281 agcctcaagc aggccaatga ggactacacc gaggagttcg acccgtcgaa gtacggcatg
     5341 gccgacagct acgacgagca gggcaactac atcttccccg agggcttcga ttccgagacc
     5401 aacgagtggc tcgagggctt cgaccagcag cgcaccgagt gggaggcccg gtacgccgag
     5461 gcggagcgcc ggcacaagat gcacaccacg cagatggaga agttcgccgc tgccgaggcc
     5521 gccgcgggcg agaagcagcc tgcagccaat ggggcgccgc ggtcagagga agcgcagtcc
     5581 ggcgggtcgc tggccagcga cgagcagttg gccgccctgc gggagaagct ggccggcagc
     5641 gcgtaactgc tttgtcgccg agactgcaat ttcgcaggga aaatgcgagt aagtccctgc
     5701 ctctcggcgc ggggacatgc tgaggatcgg gctgaccggt ggcatcggcg ccgggaagtc
     5761 gacggtgtca gccactttcg ctgagtgcgg cggcatcgtc gtcgacggcg acgtgatcgc
     5821 ccgcgaagtc gtccagcccg gcaccgaggg gctggacaag ctggtcgacg cgttcggtga
     5881 cgacattctg ctgcccgacg gcgcactgaa tcgtcctgcc ttggccgcca aggcgttcgg
     5941 cgacgaggac aagcgggcga cgctgaacgg catcgtgcac ccattggtgg ccagacgccg
     6001 cgccgagatc atctccgcgg tgtccgaaga cgaggtcgtc gtcgaagaca tcccgctgct
     6061 cgtggaaagc gggatggcgc cgctgtttcc gcttgtggtg atcgtgcacg cggaccctga
     6121 ggtgcgggtc gagcggttga tcaagcgggg gatggacgaa gccgacgccc gtgcccgtat
     6181 cgccgcgcaa gccagcgacg agcagcggcg caaggtcgcc gacgtctggc tggacaactc
     6241 cgggtcggag ggcgaactgg tggaacgcac ccggggactg tggcaccagc gcatccttcc
     6301 gttcgcccac aacgtcagca cgcgccaaac agtccgcgca gcacagcaat tggtgccgta
     6361 cgacccgacg tggcctgcgc aggcccaccg catcatcgag cgactgaaga cggcctgcgg
     6421 ccaccacgca ctccgggtcg accacatcgg ctcgacggca atatccggga tggacgccaa
     6481 gaacgtgatc gacgtgcaga tcaccgtggc gtcgctggcg gttgccgacg agctcgccga
     6541 cacgctggcc gatatcgggt acccacgtat cgagggcatc accagtgacg tcccgcacag
     6601 cgatcagccc tcgttgtggc gcaagaggat tcacgcgtcc gccgatcccg gccgccctgc
     6661 tcacatccat gttcgcgtcg acagctggcc caatcagcag ttcgcgctgc tgtttacgga
     6721 ctggctcaga gccaacccgg gcgttcggga ggattacctg gccgcgaagc gcagagccgt
     6781 cgccacgccg gaatacaccg aagccaaaga gccgtggttc ctcgacaact accgccgggc
     6841 atgggagtgg gccgactcga ccggctggag cccgtagcgt cagttcggcg gcgcgacctc
     6901 gctgggtgat gcctcaggcg ccggctcggg aactgcagtg gtgttgacgg cctcgccggc
     6961 gtcgaccgcg gcggcgttgg caagcgggcc gccacaggcc atcacgccgt agccggggtc
     7021 gttgctctgg ggcaggaacc tcggcgcgat gaagtcgtcg gcctgggcgg tgagcgcgtc
     7081 gtcgaccagg atttcgcagt gcaggttggc ggtatacggc catctgatcg agatctccat
     7141 gccggcgtgc gcagggtcgg ccatcacggc gttcgcctcg aatgtctggc cgggcagcac
     7201 cgtcggattg gcggtctcga ccgcggtgtc actgacgttg taggtgatgg tcaggccacg
     7261 ggaaacaccg tcgattcggg cgcgataggt cacgttgtgc atttcctgcg gcagctgttc
     7321 gtccgccagc gcagtagacg gtagaaactg gccgcctgcg accaatgccg ccagcaaggt
     7381 cgtcgtcagg caggcccgga tggtgacact catgactgct gagattacgc gccagcccag
     7441 gtcaggtgca tgccgtggcc ggaaagccag cggtgaacgc agtggccgtt ctccgacagg
     7501 ccctcgatca cgtggactgc gcgctggatg gcctgctccg ccgtctgcga cgacacgagc
     7561 ccgtggtggt gggcgtccag cagctcgtcg acgtccagca gatcggtgcg ctcattggcg
     7621 taagtgacca ggtcaagata gtggtcctcg gcgcgccatt cggtttcgcc ggggatgatc
     7681 tcgccgatgt cgaggtagaa atcctgttcg cgttcgtggc cggggttgaa gtggaagacg
     7741 ttgacgcgca gccccagcga cggcagcagc cacgattcca ggtagtggaa ttgcgcgcgg
     7801 ccgggcgtcg gccgggccat gtagaggccc cacggtttca ccgtgtagac gtcgaccgcg
     7861 cgcacgatgc ccttcgggtc ggtgttggtg cgcgcgacaa ggtcgaacgt ttcgcgtttg
     7921 ggtgggtgga tacgggtcag cataggcggg gacagtggga acagaacatg tcggtggctg
     7981 gttctaggct ggtgaacatg gctttcgcga ccgaacaccc cgtactggcg cactcggagt
     8041 accgggctgt cgaggaggcg gtgcgcaccg gcggacgctt cgaggtggtc agccagcacc
     8101 agccggcagg ggaccagcct gccgccatcg acgagctgga acgccgcatc aagtccggcg
     8161 agcgcgacat cgtgctgctc ggtgccaccg gcaccggtaa gtcggccacc acggcatggc
     8221 tgatcgagcg gctgcagcgg cccacgctgg tgatggcgcc gaacaagacg ctggccgccc
     8281 aactggcgaa cgaactgcga gagatgttgc cgcacaacgc cgtcgagtac ttcgtgtcgt
     8341 actacgacta ctaccagccc gaggcgtaca tcgcgcagac cgacacctac atcgagaagg
     8401 acagctcgat caacgacgac gtcgagcggt tgcggcactc ggccacgtcg agtctgc
//
LOCUS       DASWBF010000581        15618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_323822,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000581 DASWBF010000000
VERSION     DASWBF010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15618
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323822"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VF874_21705"
     CDS             complement(<1..1224)
                     /locus_tag="VF874_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX9178579.1"
                     /translation="MTHAAQTGSTVGNPARVLREDAIGTPPKRPILVPAERYYSAAFA
                     QLEAERMWPKVWQVTCTVDHVAEPGDYFEYRCGPYSVLVIRGDDGALRAFQNVCRHRG
                     NSLCAGSGSDLRELRCGYHGWTWDLSGVLKRVPNRKGFGALPMSELPLVAVSVDTWER
                     LVFVNLDVNAGPLIAFLEAVPHDIAWNVLGDFRCYATMTIEVDANWKTIADGYSETYH
                     VQTLHPELHRCMDDVYAPQVIWGHAGKSEQLYGVQSPHIKDTLTDSDVWDAYVCTQGA
                     LMGVEEGTPLPRDQVEPGKSVADVIAERTRAFAAGRGVDLSWANTDQVMRLHQYNVFP
                     NLTLLTNADHLTVMTSLPGPDPDHGELVMMLWTRMPPGAPRSKPADVRMKADEAHPGL
                     VLTQDITVLAGLQRGL"
     gene            1313..3073
                     /locus_tag="VF874_21710"
     CDS             1313..3073
                     /locus_tag="VF874_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9178580.1"
                     /translation="MFDLKITGGTVVDGTGADRFDADVAVKDGKIVEVRRRGPGDLPL
                     EGDAVETIDATGKVVAPGFVDIHTHYDGQVSWDDLLEPSSTHGVTTVVAGNCGVGFAP
                     VQPGREEWLIELMEGVEDIPGTALSEGITWGWESYAEYLDVIGKRELAVDIGSQIAHG
                     TVRAYAMGERGARNEPATPDDIATMSRLVREAVEAGALGFSSSRTLAHRAMDGEPVPG
                     TFAAEDELFALGRAMAAGGAAVFELAPQGAAGEDIVAPKKELEWMQRLGAEIDCALSF
                     ALIQVDADPNLWREQLDMSAAAHEAGSRLHPQVAARPFGMLLGFPGHHAFTHRPTYQR
                     LKAECSREELAARLAEPAIRAAILSEDDLPIDPNVLFDGMFAFAQYSADRLYALGEPP
                     DYEPTADRTVAAIARERGEDPLTTMYDLMLEADAGAMLMMPLFNYAEGNHDAIREMMT
                     HPAGVLGLSDGGAHCSMICDASYPTFLLTHWARDRHRGEKLPLEYVIRKQSHDTAQLF
                     GLSDRGVIEVGKKADVNVIDMNALTLHAPRMAYDLPAGGHRLVQGASGYSATIVDGVV
                     TRRDGGDTGARPGRLVRGAR"
     gene            3128..4291
                     /locus_tag="VF874_21715"
     CDS             3128..4291
                     /locus_tag="VF874_21715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9178581.1"
                     /translation="MARLRFGYFIAPFHRAGTNPTLALQRDLEFVEHLDALGFDEVWF
                     GEHHSAGSEIISSPEIFIAAAAERAKKIRFGTGVISLAYHNPLWVADRLTLLDHLTHG
                     RVIGGMGPGSLPTDSAMIGLTPTDTRELLETNLDIVVRLLAGETVTAKTPTHELFDAR
                     LQLAPYSEPGIPLAVAAVASPTGARLAGKHGIGLLSIGATLVVEGFDALAYHWGIVEE
                     RAAAFGTQVDRSNWTLVGPFHIAETDEQARADVRFGIEPWFRYFQKVAAFPQMTIPGD
                     QVDEMINIINDAGAGVIGTPERARAQVQRLWDQSGGFGCMLQMGHEWANPAATKRSAE
                     LFAAEIMPHFQGQAQPTLDAAMRASDVREDLAQTQLQAIEHMTKKYQDEVGPS"
     gene            4306..5007
                     /locus_tag="VF874_21720"
     CDS             4306..5007
                     /locus_tag="VF874_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX9178582.1"
                     /translation="MRALLFDTFGTVVDWRGGLISSMQAWGVARGVAADWPGLVDAWR
                     AEYVPSMAKVRTGEREWADLDVLQRESVEILAPKFGASGLDGHALDELVRMWHELPPW
                     PDSVEGLRRLRTRYVIAPLSNGHVALLVRLAKAAGITWDVVFGADVFRHYKPDPDTYL
                     GAAALLGCEPSEVMLVAAHGSDLDAAASCGLRTCYVSRPLEYGPDRAVEKWPATGRFD
                     IRVRDLVELAVEMGC"
     gene            5048..5224
                     /locus_tag="VF874_21725"
     CDS             5048..5224
                     /locus_tag="VF874_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178583.1"
                     /translation="MANFATELSGLAGRDTDTDAAQWIIRVVLSLLFWPIGDDDVENQ
                     MLERFVALGFTEQG"
     gene            complement(5312..5455)
                     /locus_tag="VF874_21730"
     CDS             complement(5312..5455)
                     /locus_tag="VF874_21730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178584.1"
                     /translation="MSEHPEPKPEESEEANEETTENKARGLIQYPPKPGQTQYGTYQN
                     PHH"
     gene            complement(5671..7134)
                     /locus_tag="VF874_21735"
     CDS             complement(5671..7134)
                     /locus_tag="VF874_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX9178585.1"
                     /translation="MSAAPDTTVALQDRFSSELPEMSVRWHAEPLTNPQLLMLNEPLA
                     RQLGLDAAWLRSSNGLRFLVGALVPSGATPVAQAYAGHQFGGFVPRLGDGRALLLGEL
                     VDADGRVRDIHLKGSGPTPFARGGDGLAVVGPMLREYVVSEAMHALGIPTTRSLAVVG
                     TGRPVQRETLLPGAVLTRVASSHLRVGSFQYAASTGDVDLLRRLADHAIARHHPTAAA
                     AERPYLALFEAVITVQASLLARWMLVGFVHGVMNTDNMTISGETIDYGPCAFMEAYHP
                     NTVFSSIDAWGRYAYGNQPSVAGWNLARFADALLPLFSDDQEQAIALAEKALGGFPDQ
                     YDTAWSMGMQAKLGLPWHLEVEVVKPLVDELLEMLQQDNVDFTSFFRRLGHAARGNAE
                     PARELLVDLTGFDGWLKRWRALDPDAGLMDRSNPMYIARNHLVEEALTAATAGDLEPL
                     EQLLAAVIDPYDARPGLERYAAAAPEEFGSYRTFCGT"
     gene            7422..8264
                     /locus_tag="VF874_21740"
     CDS             7422..8264
                     /locus_tag="VF874_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9178586.1"
                     /translation="MREVSVITGGAGGIGLATAKIVGRDHFVVISDVRQDRLDAGMTE
                     LEHLDIECKAVVCDITDRNSVAELVETASALGPVTSVVHTAGVSPSMGDAEFIMRINA
                     IGTVNVNDGFYPIAGEGFAIVNVASMAAYMLPQIILPTGQFKYAFKDEDVFMRKMMSA
                     CKVAPRKLRPGLAYTISKTFVVWYCASQASKFGGRGARIVSVSPGSIDTEMGRLEEQS
                     GSGAMLRYAALKRFGRPEEIAEVLAFCASKKPGYLTGVDILCDGGVGASMTLRDKLAV
                     ARNP"
     gene            complement(8823..9638)
                     /locus_tag="VF874_21745"
     CDS             complement(8823..9638)
                     /locus_tag="VF874_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9178587.1"
                     /translation="MRVIVTGGNSGVGKATAAALAAAGHNVVIACRSIPKAEQAAADM
                     AGAVEVAQLDLADLASVRAFADSVESVDVLVNNAGVMGLPLTRTADGFEEHMGTNHLG
                     HFALTCLLGDKIKDRVISVTSAMYALSRIHLDDLNWHTRRYSKRAAYEESKLAIMLFV
                     HELTRRGVRAYLSDPGATDTDIVRTTTGLLNWLNKRSLPRPYLHTPAQGARATVQAVT
                     TDLPSGTYLAPRFNQWGKPKVTRPLKKARDPQMARRLWDLSAGLTGCDWRGAG"
     gene            10123..11001
                     /locus_tag="VF874_21750"
     CDS             10123..11001
                     /locus_tag="VF874_21750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9178588.1"
                     /translation="MRVGLHALGIGAGADRAVIDAVAKAAEDHGFATLWAGEHVVMVD
                     DPESTYPYSDDGKIAVPAVADWLDPIVCLSFAAAATTTIGVATGVLLLPEHNPVLVAK
                     QAASLDRLSNGRLTLGVGIGWSREEFDALGVPFAHRAKRTAEYVDAIRGLWRDDVASY
                     SGDFVDFHSIRVNPKPMRDRHIPIVLGGNSDTALQRVVAWGDGWYGFNLDDVAAVANR
                     VELVDKLCHAAGRDRRELSLAVALRQPQDPDVAALAELGIDELVIVEAPPDDTAAAPA
                     WVCGLAERWIGSELCA"
     gene            10992..11339
                     /locus_tag="VF874_21755"
     CDS             10992..11339
                     /locus_tag="VF874_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX9178589.1"
                     /translation="MRVARIYDPAEPGDGVRVLADRLWPRGVRKDDQRIDRWVKDVAP
                     SSELRRWYNHDLERYDEFVARYQHELEDPIAANALNELRHLAKTGPLTLLTSAKDVEH
                     GHLVVLKRLLEKN"
     gene            11339..11764
                     /locus_tag="VF874_21760"
     CDS             11339..11764
                     /locus_tag="VF874_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178590.1"
                     /translation="MVAPWVVAVAGIFVWLGMVLAISFIEAPLKFRAPGVTLQLGLGI
                     GRLVFRALNTAELVLGAAVLVALALQPPPLPVVVTVLVAVVALAVQLLAVRLRLSRRS
                     DAVLAGEDGVRSRAHYVYVGLEVIKVIALLVSGILLLAA"
     gene            11785..12132
                     /locus_tag="VF874_21765"
     CDS             11785..12132
                     /locus_tag="VF874_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9178591.1"
                     /translation="MDSLSLTAVVDEQLARARQSNSGRSAQTVYGGHEHFLRQTVIAI
                     IAGRELGEHNSPGEATLQVLQGEVKVTAGEESWQGAIGDFLVIPAVRHNLEALDDSVV
                     LLTVRADSREGIQ"
     gene            complement(12126..13217)
                     /locus_tag="VF874_21770"
     CDS             complement(12126..13217)
                     /locus_tag="VF874_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX9178592.1"
                     /translation="MGAAGPATSNDDCLKLTAYFGERQRTDGRFLAEDLLTLYGERAV
                     ATSVMLRGIASFGPRKQLRSDEWLSLSEDPPVAVAAVDARDKIAGLVEDVVEMTPRGL
                     ITLERARLITDAVVPQGVHNAAKLTVYVGRQARVDGVPAYFAICDLLYREGVAGASVF
                     LGVDGTVRGQRQRARFFSRNVDVPMMIISVGSADSIARVVPQLGAMLRQPLLTVERIR
                     VCKRDGELLSRPDALPMADDQGRPLWQKLMVYTSSAALHDGAPIHRALIRELRTSGAA
                     SGATVLRGVWGFHGDQKPHGDKLIQLARRVPVTTIIVDRPDRIARSFEIVGDVTREHG
                     LVTSEMVPAHVLVDGNERIGGTALADYRY"
     gene            complement(13220..13585)
                     /gene="crcB"
                     /locus_tag="VF874_21775"
     CDS             complement(13220..13585)
                     /gene="crcB"
                     /locus_tag="VF874_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX9178593.1"
                     /translation="MTIAVWGGVVLLGGAGAVLRFLVDRAVARRVARSFPFGTLTVNL
                     TGAVLLGLLSGLALSHHVALLAGTAFVGSYTTFSTWMLETQRLGEERQVWPAVANIVV
                     SVILGVAAAMAGQWIAGRL"
     gene            complement(13582..13977)
                     /gene="crcB"
                     /locus_tag="VF874_21780"
     CDS             complement(13582..13977)
                     /gene="crcB"
                     /locus_tag="VF874_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX9178594.1"
                     /translation="MAFDRRELAAVFAGGALGTVARAGLEMLANPDPGRWPWPTFAVN
                     VVGAFLLGYFTTRLLERLPLSSYRRPLLGTGLCGGLTTFSTMQVETLRMLEHHHYGLA
                     IGYTVASIAAGLVAVYLATALVRRVRVRA"
     gene            14121..>15618
                     /gene="pgm"
                     /locus_tag="VF874_21785"
     CDS             14121..>15618
                     /gene="pgm"
                     /locus_tag="VF874_21785"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEX9178595.1"
                     /translation="MAANPRAGHPAQPEDLIDVAHVVTAYYTVQPDPDDVAQQVAFGT
                     SGHRGSSLDGAFNEAHILATTQAIAEYRAAQGTTGPLFIGRDTHALSEPAWVSALEVL
                     AANDVAAMIDSADRYTPTPAVSHAILSHNRGRSDGLADGIVVTPSHNPPRDGGFKYNP
                     PNGGPADTDATGAIAKRANEILRAGLKAVKRVPLARALTSAQRHDYLNAYVDDLPNVV
                     DVHAIRAQGVRIGADPLGGASVDYWGAIGERHNLELTVVNPLVDATWRFMTLDTDGKI
                     RMDCSSPNAMASLISNRDKYQIATGNDADSDRHGIVTPDGGLLNPNHYLAVAIDYLYS
                     HRPSWPSSTAVGKTVVSSSIIDRVVGGLGRKLIEVPVGFKWFVDGLLSGSIGFGGEES
                     AGASFLRTDGSVWTTDKDGIILALLASEMLAVTGQTPSQRYDELAEKYGAPTYARVDA
                     PADREQKARLAKLSPEQVTATELAGEKITAKMTTAPGNGAPLGGLKVTT"
BASE COUNT         2682 a         5010 c         5233 g         2693 t
ORIGIN      
        1 cagaccgcgc tgaagtccgg ccagcaccgt gatgtcctgg gtcaagacca gaccggggtg
       61 tgcctcgtcg gccttcatcc gtacgtctgc aggcttgctg cgcggcgcac cgggcggcat
      121 ccgcgtccac aacatcatca ccagttcgcc gtgatcggga tcgggtcctg gaagtgaggt
      181 catgaccgtc aggtgatcgg cgttcgtcag aagagtcaag ttcgggaaga cgttgtactg
      241 gtgcagccgc atgacctggt cggtattggc ccagctcaaa tcgacgcccc gcccagcggc
      301 gaaagctctt gtgcgctcgg cgatcacgtc tgcgaccgac ttgcccggct caacctgatc
      361 gcgcggcaac ggggtgccct cttcaacgcc catcaacgct ccctgggtgc agacatacgc
      421 gtcccacacg tcgctgtcgg tgagcgtgtc cttgatgtgc gggctttgca caccgtaaag
      481 ctgctcggac ttgcccgcgt gtccccagat cacctgcggt gcatagacat cgtccatgca
      541 ccggtgcagc tccggatgca acgtctggac gtgataggtc tcgctgtacc cgtcggcgat
      601 cgtcttccaa ttcgcgtcga cctcgatggt catggtggcg tagcagcgga agtcgccgag
      661 cacgttccag gcgatgtcgt gcggcaccgc ttccaagaag gcgatcaagg gccccgcgtt
      721 gacgtcgaga ttgacgaaga cgagcctctc ccacgtgtcg acgctgaccg cgaccagtgg
      781 aagctcggac atcggcagcg ccccgaatcc cttacggttg ggtacccgtt tcagaacacc
      841 ggaaagatcc cacgtccagc cgtggtaacc gcaccgcagt tcacgcaagt ccgaaccaga
      901 gccggcgcac agcgagttgc cccggtggcg gcagacgttc tggaaagcgc ggagcgcacc
      961 gtcgtcgccg cgaatgacaa gtaccgagta agggccgcaa cggtattcga agtagtcgcc
     1021 gggttcggcg acgtggtcga ccgtgcaggt gacctgccac accttcggcc acatccgctc
     1081 cgcctcgagc tgtgcaaacg ccgcggagta gtagcgctcc gccggcacca ggatgggacg
     1141 tttcggcggc gtgccgatcg catcctcacg gaggacgcgg gccgggttgc cgaccgtgct
     1201 gccggtctgc gctgcgtgcg tcacgattcg ccttcctgac acggcgccac tgctctgtaa
     1261 cggtgttaca ttggccactg tagcggctgg cagggcacac aagggagtaa tcgtgttcga
     1321 cctcaagatc acaggcggga ctgtcgtgga cggaacgggc gcggaccgct tcgacgctga
     1381 tgtggctgtc aaggacggca agatcgtcga ggttcgtcgc cgcggacctg gagaccttcc
     1441 actggaaggc gacgcggtcg aaacgatcga cgccacagga aaagtcgtcg cgccgggatt
     1501 cgtggacatc cacacccact acgacggtca ggtcagctgg gatgaccttc tcgagccgtc
     1561 cagcactcac ggcgtgacca cggtcgtggc cggcaactgc ggcgtcggtt tcgcgccggt
     1621 gcagcccggc cgcgaggaat ggctcatcga gctgatggag ggcgtcgagg acattccggg
     1681 aaccgctctc tccgaaggca ttacctgggg ctgggagagc tacgcggagt acctcgatgt
     1741 gatcggcaag cgcgagctcg ccgtcgacat cggaagccag atcgcgcacg gaaccgtccg
     1801 cgcgtacgcg atgggggagc gcggcgctcg caacgagccc gcgacgccgg acgacatcgc
     1861 gacgatgagc cggttggtac gagaggcggt tgaggccggt gcactggggt tctcgtcgtc
     1921 gcgcacgctc gcccaccgcg ccatggatgg cgaacccgtc cctggaacgt tcgctgctga
     1981 agacgaactg ttcgcactcg gccgcgccat ggccgccggc ggtgctgccg tgttcgagct
     2041 cgcgccgcag ggcgcggcag gggaggacat cgtggcgccg aagaaggaac tggagtggat
     2101 gcaacggctc ggcgccgaga tcgactgtgc gttgagcttc gcgctgatcc aggtcgacgc
     2161 cgaccccaac ctgtggcgcg aacagctcga catgtccgcg gccgcccatg aggcgggcag
     2221 ccggctgcac ccgcaagtcg cggcacgccc gttcggaatg ctgctcggct tccccggtca
     2281 ccacgccttc acccaccgcc cgacctatca gcggctgaag gccgaatgca gccgggaaga
     2341 actcgccgcc cggttggccg aacccgcgat acgggcggcc atcctgtccg aggacgacct
     2401 gccgatcgac ccaaatgtgc tgttcgacgg catgtttgcg ttcgcacaat actcggccga
     2461 caggctctac gccctcggcg agccacccga ctacgagccc acagccgacc gcacagtcgc
     2521 cgcgatcgcc cgcgaacgcg gcgaggatcc cctgacgacg atgtacgatc tgatgctgga
     2581 agccgacgcg ggcgcgatgc tgatgatgcc gctcttcaac tatgccgaag gcaatcacga
     2641 cgcgatccgc gagatgatga cccatcccgc gggtgtcctg ggactctccg acggcggcgc
     2701 acattgcagc atgatctgtg acgcgtcgta tcccaccttc cttctgacgc actgggcgcg
     2761 cgatcgtcat cggggtgaga agttgcctct ggagtacgtc atccgcaagc agtctcacga
     2821 caccgcgcag ctattcgggc tctccgatcg aggtgtcatc gaggtcggaa agaaggccga
     2881 cgtcaacgtg atcgacatga atgcgctcac actgcatgcc ccccggatgg cctacgacct
     2941 gccggccggc ggtcaccgac ttgtccaggg tgcctccggc tacagcgcca cgatcgtcga
     3001 cggtgtcgtg acccgccgcg acggcggtga cacgggcgcg cgccccggtc ggttggtgcg
     3061 tggagcgcgc tgagcgagcg cgctccgctg cccgggtccg agttgtccca cgcgaaagga
     3121 ggcgtcaatg gccaggctca ggttcggtta cttcatcgca cccttccacc gcgcggggac
     3181 caaccccacg cttgcgttgc agcgcgatct ggaattcgtg gagcatcttg acgcgctcgg
     3241 cttcgacgag gtctggttcg gcgagcatca ctccgcaggc agcgagatca tcagctcacc
     3301 cgagatcttc atcgccgccg cggccgaacg ggcgaaaaag atccggttcg gcaccggcgt
     3361 catctcgctg gcctatcaca accccctgtg ggtcgccgat cgcttgacgc tgctcgacca
     3421 cctcacccac gggcgggtga tcggcgggat ggggcctggt tcgctgccca ccgactccgc
     3481 gatgatcggc ctgactccca ccgacacgcg cgaactgctc gaaaccaatc tcgacattgt
     3541 cgtgcggctg ttggccgggg agacggtgac ggcgaagacc cccacacacg aactgttcga
     3601 cgcccgtttg caactcgcgc cctattcgga gcccggcata ccgctggcag tggccgcggt
     3661 cgcctcgccg acgggtgcgc ggttggccgg caagcacggc atcggcctgc tgtccatcgg
     3721 ggcgacgctg gtcgtggagg gcttcgacgc gctcgcctac cactggggaa tcgtggaaga
     3781 gcgggcggcg gcgttcggca cgcaggtcga ccgcagtaac tggactctgg tcgggccgtt
     3841 tcacatcgcc gagaccgacg agcaggcccg cgctgacgtg agattcggaa tcgagccatg
     3901 gttccggtac ttccagaagg tggccgcctt cccgcagatg accattccgg gtgatcaggt
     3961 cgacgagatg atcaacatca tcaacgacgc cggagccggc gtgatcggca caccggagcg
     4021 tgcgcgtgca caggtgcagc ggctgtggga tcagtccggc gggttcggct gcatgctgca
     4081 gatgggccac gagtgggcga atcccgcggc caccaagcgc tccgccgaat tgttcgccgc
     4141 agagatcatg ccgcattttc agggtcaggc ccagccgaca ctggacgccg cgatgcgcgc
     4201 gagcgacgtg cgcgaggact tggcgcagac acagcttcag gcgatcgagc acatgaccaa
     4261 gaagtatcag gacgaggttg gcccgagctg actgacaatg tggctgtgcg tgcgttgctg
     4321 tttgacacgt tcgggacggt cgtcgactgg cggggaggat tgatttcctc catgcaggca
     4381 tggggagtcg cgcgcggggt cgcggcggat tggcccggac tcgtggatgc gtggcgcgca
     4441 gaatatgtgc cgtcgatggc caaggtccgg acgggtgagc gggaatgggc cgatctggat
     4501 gtgttgcagc gggagtcggt ggagattttg gcgccgaagt tcggggcatc cggactcgac
     4561 ggccatgcgc tcgacgaact tgttcggatg tggcacgagt tgccgccctg gcctgactcg
     4621 gtcgaggggc tgcggaggct tcgcacgcgt tacgtgatcg cgccgctgtc gaacgggcac
     4681 gtggccctgc tggtccggtt ggcgaaagct gccggcatca cctgggatgt ggtgttcggc
     4741 gcggatgtgt ttcggcatta caagccggat ccggacactt acctcggagc ggcagccctg
     4801 ttgggatgcg agccgtcgga agtgatgctg gtggcagcgc atggttcgga tctcgatgcg
     4861 gcggcaagct gcggactgcg gacgtgttac gtttcgcggc cgttggagta cgggcccgac
     4921 cgggcggtcg aaaagtggcc agcgacaggg cgtttcgaca taagagtgcg cgacttagtg
     4981 gagttggcgg tggaaatggg ctgctgacag ccacattgct gttagatgcc gaaatccgtg
     5041 ctcgtcggtg gctaacttcg ccaccgagct cagtggcctc gccggtcgcg acaccgacac
     5101 cgacgccgcg cagtggatca tccgtgtggt gttgtcgttg ttgttctggc cgatcggcga
     5161 cgacgacgtc gaaaaccaaa tgctggaacg gttcgtcgcg ctgggcttca ccgagcaggg
     5221 ctgaagattc gctacggccc agagggaaat gtcaccgcat gaaaacgtag gccaccgctc
     5281 cgtcgatggc cgtagctcta ctcggtgcct ctcagtggtg cgggttctgg taggtcccgt
     5341 actgcgtctg ccccggcttc ggcggatact ggattaagcc gcgcgccttg ttctccgtcg
     5401 tctcctcgtt ggcctcttcg ctttcctcgg gtttgggttc tgggtgttca gacatgttga
     5461 ccttcccttt tgcagtcacc gatgtgcggc aacacaccac aagcctacgg ctgcgctccg
     5521 aagagcgtaa gagttgagca gatccacgcg ctcgctgaat tgcccagcag gacaatcact
     5581 cgagcgccgg ccgaatcagc ttgatgcctt gccgcccttg cggccggcag ccttcttctg
     5641 agcctgattc tcgccactgc cgggcgatgc tcaagtgccg cagaaggttc ggtaggagcc
     5701 gaactcctcc ggggcagcgg cggcatagcg ctcgaggccc ggtcgtgcgt cataagggtc
     5761 gatgacagcg gcgaggagtt gctcgagtgg ctcgaggtca cccgcagtcg ccgcggtcag
     5821 ggcctcctca acgaggtggt tgcgggcgat gtacatcgga ttgctccggt ccatcaatcc
     5881 ggcatctgga tccagagcgc gccagcgctt cagccagccg tcgaacccgg tgaggtcgac
     5941 gagcagttcg cgcgcaggtt cggcgttgcc gcgggcagcg tggccgaggc ggcggaagaa
     6001 cgaggtgaag tcgacgttgt cttgctgcaa catttcgagc aactcgtcaa ccaacggctt
     6061 cacgacctca acttcaaggt gccaaggcag gcccagtttg gcctgcatgc ccatggacca
     6121 ggccgtgtcg tactggtcgg ggaaaccgcc gagtgccttc tcggcgagtg cgatcgcctg
     6181 ctcttggtcg tcggagaaca gcggaagaag cgcgtcagcg aaccgggcca ggttccaccc
     6241 cgccactgaa ggctggttcc cgtaggcgta gcggccccag gcgtcgatcg agctgaagac
     6301 tgtattgggg tggtaagcct ccatgaaggc acatggcccg tagtcaatgg tctcgccgga
     6361 gatcgtcatg ttgtcggtgt tcatcacccc gtggacgaaa cccaccagca tccatcgcgc
     6421 cagcagcgac gcctggacgg tgatgaccgc ttcgaacagc gcgaggtagg ggcgctcggc
     6481 cgccgcggcg gtgggatggt gccgcgcgat cgcgtggtcg gcgaggcgcc gcagcaggtc
     6541 gacgtcgccc gtggacgcgg cgtattggaa gctccccacc cgcaggtggc tgctggcgac
     6601 gcgggtgagg acggcgccag gcaaaagcgt ctctcgttgt accgggcgcc cggtgcccac
     6661 tacggccaag gatcgggtag tcgggatacc gagagcgtgc atcgcctcgc tgacgacgta
     6721 ttcgcgcagc atcggcccga cgacggccag gccgtcaccg ccgcgcgcga acggcgtggg
     6781 accagaaccc ttgagatgga tgtcgcgaac gcgcccgtcg gcgtcgacga gctcgccgag
     6841 cagcagcgcg cgcccgtcgc ccagccgcgg gacgaagccg ccgaactgat gcccggcgta
     6901 tgcctgggct accggagtgg cgccgctggg caccagggcg ccgaccaaga accgcagacc
     6961 gttggaactc cgcagccaag cagcgtcgag gccgagctgc ctggccagcg gctcgttgag
     7021 catgagcagc tgcgggtttg tgagcggctc ggcgtgccag cggacactca tctccggcag
     7081 ttcgctggag aatcggtcct gcagggcgac ggttgtatcc ggtgcagcgc tcacttcacc
     7141 cagactacgg acgaggccga acgtcaaatc ctcacccacg catcgacttc cgcaagcaag
     7201 actcagccaa tgcctgcggc ggccgcgtgt tcgtcacctg atggcccgcc ccatcgtcgc
     7261 cgtgaacgtc ctgcgagtcg gcgcagggta cgtatcgtct cttcccttgt gctgcgcaat
     7321 ttcctagcat cacgtcagcc gtcagcgtgc tcatgctttt ttggtcgcgg cgctgacggc
     7381 ggcagggtct ggcgttcgcg gtcgaagaga atgggtgaga gatgcgagag gtttcggtca
     7441 ttaccggcgg tgccggcggc atcgggcttg cgacggcgaa gatcgtcggg cgggatcact
     7501 tcgtcgtcat ctccgacgtc aggcaggatc gcctggacgc tgggatgacc gagctggagc
     7561 acctcgacat cgagtgcaag gccgtcgtct gtgacatcac cgatcgcaac tccgtcgctg
     7621 agctggtcga aactgcctcc gcactcggac cagtcacctc tgtagttcac accgcgggtg
     7681 tgagccccag tatgggcgac gccgagttca tcatgcgaat caacgcgatc ggcaccgtca
     7741 acgtcaacga cggcttctac ccgattgcag gcgagggctt cgcgatcgtc aatgtggcgt
     7801 ccatggcggc ctacatgttg ccccaaatca tccttccgac aggacaattc aagtacgcgt
     7861 tcaaggatga agacgtgttc atgagaaaga tgatgtcggc ctgcaaggtc gcgccgagaa
     7921 agctgcgccc cggcctggcg tacaccatca gcaagacctt cgtcgtgtgg tactgcgcat
     7981 cgcaggcatc gaagttcggt ggtcgagggg ctcgtatcgt ctcggtctct cccggctcta
     8041 tcgacacgga gatgggcaga ttagaggagc agagcggatc cggcgcgatg ctgcgttacg
     8101 ccgcactcaa acggttcggg aggccggagg agatcgcgga ggtgttggca ttctgcgcca
     8161 gcaagaaacc gggttacctc accggtgtcg acatcctctg cgacggtggg gtcggggctt
     8221 cgatgacgtt gcgagacaag ctggcggtcg cgcgcaaccc gtagatgagc ggtctgctcg
     8281 tcctagtagt tgtcagtgct cgtccagtag ttggtcagag ctttcttggg cggatgcatt
     8341 aaaggtaatc ctggaacgcc ccacggctgg gccgcacgat tgcgggctgc aaatcggaaa
     8401 cggagtttgc gatacgtgcg gatttgtcac gtaatctgtg ccacatattt acgtcaattt
     8461 aaaagaattc gcccggttca agcgaccgac aaagtcctgc gtgaagtcag ccgggcgcgg
     8521 aatgcggcgc gcagccggct gaatagtcat gcggcgaccg ccctgacctt cgccaacgtc
     8581 aattctcaag tcgcgcggga tgcgtggccg tcgtgcgccg tgccatggct caattgatac
     8641 caagttatta ccaagatgcg aacatccgct cagttgattc acagcttcaa tgagctatcg
     8701 tctaatagcc ccggtggtgc cgttgatgga caacttcggt cttaactccc cttcccgggt
     8761 gttgccgccg gggcttcacc taattgacag caccgtcact cggtgcggct caccgtgcag
     8821 atttacccgg cccctcgcca gtcgcagccc gtcagcccgg ccgagaggtc ccacaaccga
     8881 cgtgccatct gcggatcgcg ggccttcttg agcggtcgcg tcactttagg ctttccccac
     8941 tggttgaacc gcggcgcgag ataggtcccg ctgggcagat ccgtcgtcac cgcctgaaca
     9001 gtcgcccgcg ctccctgcgc tggggtgtgc aggtacggcc gcggcaagct ccgcttgttc
     9061 agccagttca gtagaccggt ggtggtcctg acgatgtccg tatccgtcgc acccggatca
     9121 ctcaggtacg cccgcacacc gcggcgggtg agctcgtgga cgaacaacat gatggccagc
     9181 ttcgactcct cgtaggccgc ccttttcgag taccggcggg tatgccagtt caggtcgtcg
     9241 agatgaatgc gactgagggc atacatcgcg ctggtcaccg agatcacccg atccttgatc
     9301 ttgtcgccga gcaggcatgt caacgcgaag tggccgaggt gattggtgcc catgtgttcc
     9361 tcaaagccgt cggcagtgcg ggtgaggggc aggcccatca cgccggcgtt gttcaccagc
     9421 acgtcaaccg attccaccga gtcggcgaag gcgcgcacac tggccaggtc cgcgagatcg
     9481 agttgggcaa cctcgacagc gccggccatg tcggcggcgg cttgctcggc cttcggaatg
     9541 ctgcggcacg cgatcaccac gttgtgccct gcagcggcca gcgccgcagc cgtcgctttg
     9601 ccgacgccgc tattgccgcc ggtgacgatg acccgcatct cggcgcctcc tccccaggta
     9661 ttcctcgtcg caggcggtca ttattacgga ctcgaaacca tggccaggcg tcggtcgtgg
     9721 tgcccgacta gttgtactga ccacagacgt tggtgacggc gtccatagag gcatcacccc
     9781 tttctgaggt cgtcatcatt tgatgacgca cgtgggaacc atgcttctcc cctgcggcgc
     9841 ccggcgtgcc ggcgatcgac cgcagcgacg ctcgtccgat gcagcgagtc cacccccgat
     9901 cgcgtccggg gccagcggcc cggggagtgc acgacgcgcc cagctgtttc gcaagccgcg
     9961 agcgctggcg aagcggtgcc tcgaccagca gcccgattgc ggtcaactgc ccgtggcggc
    10021 cgatgagttc gacgactcgg cgggaggtcg tccgtcgtgt ccgagcaagc cgccgacgcc
    10081 gcgcgacgcg gagaggcgtc gggcctatga gacgatccgg gagtgcgtgt gggactgcat
    10141 gcgctcggca tcggcgccgg cgccgaccgt gccgtgatcg acgctgtcgc caaggccgcc
    10201 gaagaccacg gcttcgccac gctgtgggca ggcgagcacg tcgtgatggt cgacgacccg
    10261 gagtcgacgt atccgtattc cgacgacggc aaaatcgcgg tgcccgccgt cgcggactgg
    10321 ctcgacccga tcgtctgcct gagtttcgcc gcggccgcca cgacgacgat cggcgtcgcc
    10381 accggcgtgc tgctgctgcc cgaacacaac ccggtgcttg tcgcgaagca ggcggcaagc
    10441 ctcgaccggc tcagcaacgg ccggttgacg ctcggcgtcg gaatcggttg gtccagagaa
    10501 gaattcgacg cgctcggcgt gccgttcgcg catcgagcaa agcgcaccgc cgaatacgtc
    10561 gacgcgatcc ggggcctctg gcgcgacgac gtcgcatcgt attcaggcga ctttgtcgac
    10621 ttccattcga taagggtgaa tccgaagccg atgcgggacc gccacatccc aattgtgctg
    10681 ggcggcaaca gcgataccgc gttgcagcga gtggtcgcct ggggtgacgg ctggtacggc
    10741 ttcaacctgg acgacgtcgc ggcggtcgct aaccgggtcg agctcgtcga caagctgtgc
    10801 catgcggcag gccgcgaccg tcgcgagctg agtttggcgg tcgccctacg gcagccgcaa
    10861 gaccccgatg ttgcggcgtt ggcggagttg ggtatcgacg aattggtgat cgtcgaggca
    10921 ccgcccgacg acaccgccgc ggcacctgcg tgggtgtgcg gtctagccga gcgctggatt
    10981 gggagcgaac tatgcgcgta gcgcggatct atgaccccgc cgaacccggt gacggtgtcc
    11041 gtgtgctggc cgaccggctc tggccgcgcg gtgtccgcaa ggacgaccag cgaatcgacc
    11101 gctgggtcaa ggacgttgcg ccgtcgtcgg agttacgtcg ttggtacaac cacgatctcg
    11161 aacgttacga cgagttcgtc gcgcgctacc aacacgagct agaagatccg atcgccgcca
    11221 atgcgctcaa cgagcttcgc cacctcgcca agactggacc gctcacactg ctcacgtccg
    11281 ccaaggatgt cgagcatggc cacctcgtcg tgctcaaacg tctcctggag aagaactgat
    11341 ggtcgcacca tgggtggtcg ctgtcgccgg catcttcgtg tggctcggca tggtgctcgc
    11401 gatctcgttc atcgaggcgc cgctgaagtt ccgggccccc ggggtcacgc ttcagctggg
    11461 tctgggcatt ggtcggctgg tgttccgcgc tctcaacacc gcggagttgg ttctcggcgc
    11521 cgccgtgctg gtggcgctgg cgctgcaacc gccaccgttg ccagtggtgg ttacggtcct
    11581 ggttgctgtt gtcgccctgg cggtgcaact gctggcggtt cggctgcggt tgagccgccg
    11641 atcggatgcg gtactggcgg gcgaggacgg ggtgcgatct cgtgcgcact acgtttacgt
    11701 cgggttggaa gtgatcaaag tgatcgcgct gctggtttcc ggcattctgt tgctggccgc
    11761 gtgacgccgc gacgaggagg agctatggat tcgttgtcat tgaccgcggt ggtcgacgaa
    11821 cagttggcaa gggcacgcca atccaacagt ggtcgatccg ctcagacggt gtacggcgga
    11881 cacgagcact tcctgcgcca gaccgtgatc gccatcatcg ccgggcgtga gctcggcgag
    11941 cacaacagcc caggggaagc cacgcttcag gtgttgcagg gcgaagtgaa ggtgacggcc
    12001 ggcgaggagt cgtggcaggg cgcgatcggt gacttcctcg tgattcccgc cgtccggcat
    12061 aacctcgagg cactcgacga ctcggttgtg ctgctcaccg tgcgggccga cagccgcgaa
    12121 ggcattcagt aacggtagtc ggccagtgct gtgccgccga tccgctcgtt gccgtcgacg
    12181 aggacgtgcg ccggcaccat ttcgctggtc accagcccgt gttcgcgtgt gacgtcgccg
    12241 acgatctcga agctgcgcgc gatgcggtcc ggccggtcga cgatgatcgt ggtcaccgga
    12301 actcgccggg ccagctgaat cagcttgtcg ccgtgcggtt tctgatcgcc gtggaagccc
    12361 cagacacccc gcagcacggt cgccccgctg gcggcgccgg aggtgcgcag ttcgcggatc
    12421 agcgctcggt gaatcggcgc tccgtcgtga agcgcggcag aggacgtgta gaccatcagc
    12481 ttctgccaca gcggtcggcc ttggtcgtcg gccatcggca gtgcgtccgg ccggctcaac
    12541 agttcaccgt cgcgtttgca tacgcgtatc cgctccacgg tgagaagtgg ttggcgcagc
    12601 atcgcgccga gctgcggcac cacgcgcgcg atgctgtcgg cgctgcccac cgagatgatc
    12661 atcatcggca catcgacgtt gcggctgaag aaccgggcac gctgccgctg gccccgcacg
    12721 gtcccgtcga cgccgaggaa caccgacgcg ccggcgactc cctcgcggta gagcagatcg
    12781 cagatcgcga agtaggccgg taccccgtcg actcgcgctt ggcggccgac gtagacggtc
    12841 agcttggcgg cgttgtgaac accctgtggc acaacggcgt cggtgatcag ccgggcccgc
    12901 tccagcgtga tcagcccgcg tggtgtcatc tcgacgacat cctcgacgag gcctgcgatc
    12961 ttgtcgcggg cgtcgaccgc ggcgacagcg acgggcgggt cctccgacag gctgagccac
    13021 tcgtcgctgc gcagctgctt gcgcgggccg aagctggcga tgccgcgcag catcacgctc
    13081 gtggcgacgg cccgctcgcc gtacaacgtc agcaggtcct cggccaggaa gcggccatcg
    13141 gtgcgttggc gctcaccgaa gtaggcggtc agcttcaggc aatcgtcgtt cgatgtcgct
    13201 ggcccagcgg ctccgatgct cacagccggc ccgcgatcca ctgaccggcc atagcggcgg
    13261 cgacgccgag aatcacgctg acgacgatgt tggcgacggc tggccacacc tgccgctcct
    13321 cgccgagccg ttgggtttcc agcatccagg tggagaacgt ggtgtaggac ccgacgaacg
    13381 cggtgccggc cagcagcgcc acatggtggc tcagcgccag ccccgacagg agaccgagta
    13441 gcacagcgcc ggtcaggttc acggttagcg tgccgaacgg gaacgaccgt gcgacccggc
    13501 gggcgacggc gcggtcgacg aggaagcgca gcaccgcacc cgcgccgccg agcagcacga
    13561 ctccgcccca caccgcgatc gtcatgcccg cacccgcacc cggcgcacca gcgcggtcgc
    13621 cagatacacc gcgacgagtc cggccgcgat actcgcgacg gtgtagccga tggccaggcc
    13681 gtaatggtgg tgttcgagca tgcgaagcgt ttccacctgc atggtcgaga acgtcgtcaa
    13741 cccgccgcac aacccggtgc ccagcagcgg tcggcggtag ctcgacagtg gcagccgttc
    13801 cagcaaccgg gtggtgaagt agcccagcag gaacgcgccg acgacgttga ccgcgaacgt
    13861 gggccacggc cagcgtcccg ggtcggggtt ggcgagcatc tccaggccgg cgcgcgcgac
    13921 agtgccgagc gctccgccgg cgaagactgc cgccagctcg cgtcgatcga aggccatgac
    13981 gcaaagtatc gcgaacgatc tgcgccgatt ttctgcacca gggtcgtcgc tgggcgccga
    14041 atcacaaccc tggtgcagaa ctcggcggcc aaccggtacc gacgaacgtc gccgcgcgtc
    14101 gaaggagcag aatcggtgac atggccgcca accctcgtgc cggtcacccg gcgcaacctg
    14161 aagacctgat cgacgtcgct catgtggtga cggcgtacta caccgtgcag cccgaccccg
    14221 acgacgtggc gcagcaggtg gcgttcggca cctccgggca ccgcggctcc agcctcgacg
    14281 gcgcgttcaa cgaggcgcac atcctcgcca ccacgcaggc catcgccgag taccgcgccg
    14341 cgcagggcac caccgggccg ctgttcatcg gccgcgacac ccatgcgctg tcggagccgg
    14401 cgtgggtgtc ggcgctggaa gtgcttgccg cgaatgatgt tgcggcgatg atcgattcgg
    14461 ccgatcgcta cacacccact cccgcggtca gccacgcgat cctgtcccac aaccggggtc
    14521 ggtcggatgg tctcgccgac gggatcgtcg tcaccccgtc gcacaacccg ccccgcgatg
    14581 ggggattcaa gtacaacccg cccaacggcg ggccggccga cactgacgct accggtgcaa
    14641 tcgccaagcg cgccaacgag attctgcggg ccgggctgaa ggcggtgaag cgggttccgc
    14701 tggctcgcgc gctgacgtca gcgcagcgcc acgactactt gaacgcctac gtcgacgatc
    14761 tgccgaacgt ggtggacgtc cacgcgatcc gcgcgcaggg tgtgcgcatc ggcgccgacc
    14821 cgctcggcgg tgcgagcgtc gactactggg gcgcgatcgg cgagcgtcac aacctggaac
    14881 tcaccgtggt caatccactg gtggacgcga cgtggcggtt catgacgctg gacaccgacg
    14941 gcaagatccg gatggactgc agctctccga acgcgatggc gtcgctgatc agcaaccgcg
    15001 acaagtacca gatcgcgacc ggcaacgacg ccgactccga ccggcacggc atcgtcacgc
    15061 ccgacggagg actgctcaac ccgaatcact atctcgcggt ggcgatcgac tacctctaca
    15121 gccaccggcc gtcgtggccg tcgtcgaccg cggtgggcaa gacggtggtg agcagctcga
    15181 tcatcgaccg cgtggtcggc gggctgggcc gcaagctgat cgaggttccg gtcggtttca
    15241 agtggttcgt cgacggattg ctcagcggga gcatcggttt cggtggcgag gagagtgcgg
    15301 gcgcgtcgtt cctgcgcacc gacgggtcgg tgtggaccac cgacaaggac gggatcatcc
    15361 tggcactgct ggcgtcggag atgctggctg tcacggggca gacgccgtcg cagcggtatg
    15421 acgaactggc cgaaaagtac ggtgccccaa cgtatgcccg ggttgacgcg cccgccgacc
    15481 gcgagcagaa ggcgcggctg gccaagctgt cgcccgagca ggtgactgcg acggagctgg
    15541 cgggcgagaa gatcacggcc aagatgacga cggcgccggg caacggtgcg ccgctgggcg
    15601 ggctgaaggt cacgacgg
//
LOCUS       DASWBF010000582         5425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_323897,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000582 DASWBF010000000
VERSION     DASWBF010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323897"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..1669)
                     /gene="tyrS"
                     /locus_tag="VF874_21790"
     CDS             complement(398..1669)
                     /gene="tyrS"
                     /locus_tag="VF874_21790"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9178596.1"
                     /translation="MSRTIVDELSWRGLIAQSTDLDALAAAVATPPITVYAGFDPTAP
                     SLHAGHLVPLLTLRRFQQFGHRPIVLAGGATGMIGDPRDTGERVLNTTDTVAEWAERI
                     RGQLERFVDFDGPNGAIVENNLSWTSQLTAIDFLRDIGKHFSVNVMLDRDTIRRRLEG
                     EGISYTEFSYMLLQANDYVELFERHRCTLQIGGSDQWGNIIAGVRLVRQKLGASVHAL
                     TVPLVTSADGTKFGKSTGGGNLWLDPEMTSPYAWYQYFVNTADADVVRYLRWFTFLSA
                     DDIAELEEATATRPHERVAQRRLAWELTTLVHGEAATSAVEHASQALFGRGELSRLDE
                     STLTAALREASVAELKPGAPDGIVDLLVASGLSPSKGAARRTIAEGGVSVNNTRIDTE
                     DWAPGPEDFLHGRWLVLRRGKRNIAGAERVE"
     gene            complement(1694..2305)
                     /locus_tag="VF874_21795"
     CDS             complement(1694..2305)
                     /locus_tag="VF874_21795"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX9178597.1"
                     /translation="MEANPLVVDPPTAARRLLGARLVTEEIAATIVEVEAYGGPPDGP
                     WPDPASHSFRGPNGRNGVMFGRAGRLYTYRSHGIHVCANVVCGPDGAAAAVLLRAAVI
                     DDGIDLARARRGAVVRDRALARGPGNLCSALAITMDHYGTDLFDPPSPIRLHLGDALA
                     AANGPRVGVSQAADRSWRFWLADHPEVSAYRRSPRAPAPGASE"
     gene            2365..3129
                     /locus_tag="VF874_21800"
     CDS             2365..3129
                     /locus_tag="VF874_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9178598.1"
                     /translation="MMSSSSDELLRNGAEPVVSIDQLRVIRGKRPAIHELSVQIARGT
                     ITGLLGPSGCGKTTLMRSIVGTQIITAGSVTVLGQPAGSAQLRRRVGYMPQDPTIYND
                     LRIVDNVRYFASLYGMDASAADAAINAVGLHDHRTAYSGNLSGGQRTRVSLACALVCH
                     PEVLVLDEPTVGLDPVLRADLWEQFNALAGAGTTLLVSSHVMDEADHCGDLLLLREGH
                     LVAHTTPTQLREDTGCSSLEEAFLSIIRRSTERQAG"
     gene            3132..3881
                     /locus_tag="VF874_21805"
     CDS             3132..3881
                     /locus_tag="VF874_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9178599.1"
                     /translation="MHAYAASTARILRQLAADRRSIAMILLVPVLIITLMYFMFQNIP
                     TRPGAPSGFNTACLILLGLFPLFVMFVITAITMQRERASGTLERILTTPLRRLELLAA
                     YGSAFSIAATAQATVACTVAFWLLGFTTAGSPAWVFVIAIVNAVLGVGLGLLCSAFAR
                     TEFQAVQFIPLVMVPQLLLAGIIVPRAAMPEWLQWISNVMPASYALEALQQVGAHPEL
                     TGIAVRDIVVVIGCAIVAMALAAATLRRRTP"
     gene            3883..4503
                     /locus_tag="VF874_21810"
     CDS             3883..4503
                     /locus_tag="VF874_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9178600.1"
                     /translation="MTAQAQRRRPGRPAGTSDTRERILVSARELFARNGIDKTSIRAV
                     AAAAGVDPALVHHYYGTKQQLFAAAIHVQIDPMQVIGPLLETPVEEIGYTLPKLLLPL
                     WDSEMGKGFIATIRSLLAGSEVSLIRSFLQEVIAAEIGPRVDNPPGSGRIRIQFVASQ
                     LVGVVMARYILELDPFKSLPVDQIAETIGPALQRYLTGDLPGWPAT"
     gene            complement(4469..4687)
                     /locus_tag="VF874_21815"
     CDS             complement(4469..4687)
                     /locus_tag="VF874_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX9178601.1"
                     /translation="MGELDEKLLAILVCPADRGPLLYVDNVLYNPRLRRAYRIDDGIP
                     VLLIDEAVDVSDDEHERLMSLANPEDRR"
     gene            complement(4690..5190)
                     /locus_tag="VF874_21820"
     CDS             complement(4690..5190)
                     /locus_tag="VF874_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178602.1"
                     /translation="MTLEPTNGLRRTVAAALAGTGLALALAFGPAAGTASADVLDDLA
                     QEFTTAAGGGQVSGLLNQSLKLRAMGMKPTGAEIAAVQDALKYRPNQTPMVNALKDMV
                     SGQSHRLEQAQAAADQGGFTVGINQYDPSSPGGVTVGPGGINIGGGSWTIGDGRSPGQ
                     AIGPPG"
BASE COUNT          919 a         1822 c         1749 g          935 t
ORIGIN      
        1 gccagcgttc gtcctgagcc aggatcaaac tctccaaaca aaaaccaaaa aggcagttca
       61 gaacaatctg acctaacaaa accagacaac atctggccaa aaacatcaca cccctaaacg
      121 ggaaaaagag gcatgaaaaa acaacaacaa acaaaaacca ccaaacacac tattgagttc
      181 tcaaacaaca cacccgcgcc ggccgcgcat ccagcccgcg gccctggggc cgcatcctgg
      241 tcgtgtgcgg acggcgagga ggccaccaaa gtggctctcc ctctgggatg ccatcgcgct
      301 cggcggactg gccgcgtcgc gacggcttca ctaaattacg tgagggacaa ctcggagtca
      361 aacaacctga tggagcgtag gtttcgactc cgccgagtca ttcaacgcgt tccgcgcccg
      421 cgatattccg cttgccgcgt cgcaggacca gccaccgccc gtgcaggaag tcctccgggc
      481 ccggcgccca gtcctcggtg tcgatccggg tgttgttgac cgagacgccg ccctcggcaa
      541 tcgtccgccg cgcggcgccc ttgctcggcg acagtccgct ggccaccagc aggtcgacga
      601 tcccgtcggg cgcgccaggc ttgagttctg cgaccgacgc ctcgcgaagc gccgccgtga
      661 gcgtcgactc gtccagccgg gacagctcac cgcggccgaa caacgcctgg ctggcgtgct
      721 ccaccgcgct cgtcgcggcc tcgccgtgca ccagcgtggt gagctcccac gccagccgcc
      781 gctgcgccac ccgctcgtgc ggccgcgtcg ccgtcgcctc ctccaactca gcgatgtcgt
      841 cagccgacag gaacgtgaac cagcgcaggt agcggacgac gtcggcgtcg gcggtgttga
      901 cgaagtactg gtaccaggcg tacgggctcg tcatttccgg gtccagccag agattgccac
      961 cgcccgttga cttgccgaac ttcgtgccgt ccgccgacgt caccagtgga acggtcagcg
     1021 cgtgcacgct cgcgcccagc ttctgacgaa ccagccgcac gcccgcgatg atgttgcccc
     1081 actggtcaga gccgccgatc tgcagcgtgc aacggtggcg ctcgaacagt tcgacgtagt
     1141 cgttggcctg caacagcatg tagctgaatt cggtgtacga aatcccctct ccctcgagcc
     1201 ggcgcctgat cgtgtcgcgg tcgagcatca cgttgaccga gaagtgcttg ccgatgtcgc
     1261 gcaggaagtc gatcgcggtc agctgggatg tccacgacag gttgttttcg acgatcgcgc
     1321 cgttgggccc gtcgaagtcg acgaaccgct ccagttggcc gcggatccgt tctgcccact
     1381 cggccacggt gtccgtcgtg ttgagcaccc gctctccggt atcgcgaggg tcgccgatca
     1441 tcccggtggc gccgccggcc agcacgatcg ggcggtgccc gaactgctga aaccgacgca
     1501 gcgtcagcag cggcaccaga tgtccggcat gcaagctcgg cgcggtgggg tcgaagccgg
     1561 cgtacacggt gatcggcggc gtggcgaccg cggcggcgag cgcgtcgaga tcggtggact
     1621 gcgcgatcag tccgcgccag ctcagttcgt cgacgatggt ccggctcatt ccgtcgatct
     1681 ttccagcagg tgcctactcg ctagctccgg gcgcgggtgc acgcgggctg cggcggtacg
     1741 cagatacctc cggatggtcg gccaaccaga accgccatga ccgatccgcg gcctggctga
     1801 ccccgacacg cgggccattc gccgcggcga gtgcgtcacc gagatgaaga cgaattgggc
     1861 tcggcgggtc gaacaggtcg gtgccgtagt ggtccatagt gatcgcgagc gccgagcaca
     1921 gattccccgg cccgcgcgcg agcgcgcgat cgcgcacgac cgctccgcga cgagcgcggg
     1981 cgaggtcgat gccgtcgtcg atcaccgcag cccgcagcag caccgcggcc gcggccccgt
     2041 ctggtccgca caccacgttc gcgcacacat ggatcccgtg gctgcggtac gtgtacagcc
     2101 gacccgcccg gccgaacatc acgccgttgc gcccgttggg gccacggaag gaatgcgacg
     2161 cggggtcggg ccagggtccg tcgggcgggc cgccgtaggc ctcgacttcg acgattgtcg
     2221 cggcgatttc ctctgtgaca agtcgggcgc cgagcagccg cctagccgca gtgggcgggt
     2281 cgacgacgag cgggttggct tccaccgaac aaattgtgcc aacccctatt gacagcacca
     2341 ggccccggga gcactattca acacatgatg agttcatcaa gcgatgaatt attgcggaac
     2401 ggggccgagc ccgtcgtttc catcgatcaa ctgcgcgtca tccggggcaa gcgccccgcg
     2461 attcacgagc tgtccgtgca gatcgcgcgc ggcacgatta ccggcctcct cggcccgtcc
     2521 ggatgcggga aaacgacgtt gatgcgctcg atcgtcggca cgcagatcat caccgcaggg
     2581 tctgtcacgg tgctcggtca acccgccggt tccgcgcaac tgcgccgccg cgtcgggtac
     2641 atgccgcagg atccgacgat ctacaacgac ctgcggatcg ttgacaacgt gcgctacttc
     2701 gcgtcgctgt acggcatgga cgcgagcgcc gccgacgccg cgatcaacgc ggtcggtttg
     2761 catgatcacc ggacggccta cagcggcaac ctgtctggcg gacagcgaac gcgggtgtcg
     2821 ttggcgtgtg cgttggtctg ccatcccgag gttttggtcc tcgacgaacc gacggtcggc
     2881 ctcgatcccg tgctgcgggc cgacctctgg gagcagttca acgcgctggc cggggccggc
     2941 accacgctgc tggtgtccag ccacgtgatg gacgaagccg accactgcgg tgacctactg
     3001 ctgctgcgcg aaggccatct ggtggcccac acgacgccga cccaacttcg agaggacaca
     3061 ggatgcagct cactggagga agcgtttctg tccatcatca ggcgcagcac cgagcgccaa
     3121 gccggatagg actgcacgcc tacgccgcca gcaccgcgcg catcctgcgt cagttggccg
     3181 ccgaccggcg cagcatagcg atgatcctgc tggtgccggt cctcatcatc acgctgatgt
     3241 acttcatgtt ccagaacatt ccgacgcggc ctggcgcgcc gtcggggttc aacaccgcct
     3301 gcctgatttt gctcgggctg tttccgttgt tcgtgatgtt tgtgatcacg gcgataacca
     3361 tgcagcgcga aagggcctcg ggaacattgg aacgcatcct cacgactccg ttgcgccgac
     3421 tggagctgct cgccgcctac ggctccgcgt tctcgatcgc agcgactgcg caggcaaccg
     3481 tggcctgcac cgtggcgttc tggctgttgg ggttcaccac tgcgggcagc ccggcatggg
     3541 tgttcgtcat tgcgattgtc aacgccgtgc ttggcgtcgg gctcggcctg ttgtgtagtg
     3601 cgttcgcccg cacggagttt caagcggtgc agttcatacc gctggtgatg gtgccgcagc
     3661 tattgctggc cggcatcatc gtgccccgcg cggcgatgcc cgaatggctg cagtggattt
     3721 ccaatgtcat gcccgccagt tacgcgctcg aggctcttca gcaggttggc gcccatcccg
     3781 agctcaccgg catcgcggtg cgggacatcg tcgtcgtcat cggatgcgcg atcgtcgcga
     3841 tggcgctggc ggctgcgaca ctacggcgac ggaccccgta agttgaccgc acaggcgcag
     3901 cggagacgcc ccgggcgacc ggccggcact tcggacacac gggagcggat actggtcagc
     3961 gcccgggaac tgtttgcgcg caacggaatt gacaagacgt cgatccgtgc ggtcgcagcc
     4021 gcagcgggag tcgatccagc gttggtgcat cattactacg gtactaagca gcagctgttc
     4081 gccgccgcca tccatgtaca gatcgatccc atgcaggtga tcggcccgct actggaaacg
     4141 ccggtcgagg agatcggcta caccttgcct aaattgcttc tgccgctgtg ggattcggag
     4201 atgggcaagg gattcatcgc gacgatccgc tctttgctgg cgggttcgga ggtcagcctg
     4261 atccgctcgt tccttcaaga ggtgatcgct gcagagattg gcccgcgcgt tgataatccg
     4321 cccggctcgg gaaggatccg gatccagttc gtggcgtcac aactcgtcgg cgtcgtgatg
     4381 gcgcggtaca tcctcgagtt ggacccgttc aagtcgctgc cggtcgatca gatcgccgag
     4441 acgatcggcc ccgctctgca gcgttatctc accggcgatc ttccgggttg gccagcgaca
     4501 taagccgctc gtgctcgtcg tcggacacgt cgacggcttc gtcgatcaag agaaccggaa
     4561 tgccgtcgtc gatccggtac gcgcggcgca accgcgggtt gtacaggacg ttgtccacgt
     4621 acagcagcgg cccgcggtcg gccgggcaga ccagaatggc gagcagtttc tcgtcgagct
     4681 cccccacgat cagccgggcg gaccgattgc ctgccccggc gaacggccgt cgccgatggt
     4741 ccacgagccg ccgccgatgt tgatgccgcc cgggccgacc gtgaccccac ctgggctgct
     4801 cgggtcgtac tggttgatgc cgaccgtgaa gccaccctga tcggccgcgg cctgcgcctg
     4861 ctccaggcga tggctctgac ccgagaccat gtccttgagg gcattgacca tcggcgtctg
     4921 gttgggccgg tacttcagcg cgtcctggac ggcggcgatc tccgcgccgg tgggcttcat
     4981 gcccatcgcg cgcagcttca acgactggtt cagcaggccg gacacctggc caccccccgc
     5041 cgcggtggtg aactcctggg ccagatcgtc gaggacgtcg gcgctcgccg tgcccgcagc
     5101 gggaccgaac gcgagcgcca gggccagtcc ggtgccggca agtgccgcgg cgacggtccg
     5161 tcgcaagccg ttcgtcggct caagtgtcat gcgatccctc cgaaagccga gtgcaagacg
     5221 ggcggtgcca cgacgggagc ttaacagtgc ctgtccggtc aggcatcagt tacaggagtc
     5281 tcttacagtg actaatggga cgtctgttcc cactcggcgc gcgcgggggc ggtcagccgc
     5341 ttgtaccggt tggtcttcgg atcgaggtac caggcgtccc gccccgcctt ggggatgccg
     5401 gcgttgcgca gagcgccgac cgtcg
//
LOCUS       DASWBF010000583        11078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1111902,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000583 DASWBF010000000
VERSION     DASWBF010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111902"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VF874_21825"
     CDS             complement(<1..275)
                     /locus_tag="VF874_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX9178603.1"
                     /translation="MTAVEPETVYTFCRYCLAACGIAVTVEDNRVIKISADKDNPHSW
                     QDFCAKGRTANQMVEHPRRILNPMRRVGDSYVEATWDEAITDIAARMT"
     gene            complement(294..2195)
                     /locus_tag="VF874_21830"
     CDS             complement(294..2195)
                     /locus_tag="VF874_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9178604.1"
                     /translation="MSAPMRMMQGGMPSAPERSRDFRGAAIRLLKRLTPQRGLTAAVI
                     GLGIVGIAIGVIGPRILGHATDLLFNGVIGRQLPAGITKEQAVEAARARGDGTFADLL
                     SRMNVTPGVGVDFAAIGRTLLLALGLYLLAAVLVWAQFRLLNVTVQRTMVTLRADVED
                     KVHRLPLSYFDSRQRGEVLSRVTNDVDNIQTSLSMTISQLLTSVLTVVAVLVMMLTIS
                     PLLAMITVLTVPLSLWVTRTIARRSRRLFVAQWTNTGRLNAHIEETYSGFTLVKTFGH
                     RAHAQQRFRELNGDLYDASFGAQFFSGLVSPFTMFIGNLSYVAVAVVGGLQVATGQIT
                     LGSIQAFTQYVRQFNQPLTQVAAMYNTLQSGMASAERVFDLLDTEEQTPDTGLPLPSV
                     NGRVGRVEFERVNFSYRRGTPVIEDLSFVADPGSTVAIVGPTGAGKTTLVNLLMRFYE
                     VDSGRILVDGVDVATVSRQSLRSRIGMVLQDTWLFGGSIAENIRYGRPGAGDDEVLAA
                     ARAAYVDRFVHTLPEGYDTPVSDDGGNISAGEKQLITIARAFLAQPQLLILDEATSSV
                     DTRTEVLVQHAMSELRRDRTSFIIAHRLSTIRDADVILVMDDGRIVERGSHTELMAKR
                     GAYWAMTQA"
     gene            complement(2192..3922)
                     /locus_tag="VF874_21835"
     CDS             complement(2192..3922)
                     /locus_tag="VF874_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9178605.1"
                     /translation="MLAGLLREYVRPYRSLVGIVAALQVISTLAALYLPTVNAAIIDD
                     GVAKGDTQTIMRLGGVMLAVSALQVLCAVAAVYFGSRTGMGFGRDLRWAIFDHVTGFS
                     AHETARFGAPSLLTRTTNDVQQIQLLVQLTCTMLITAPIMCIGGIVMALHQDAGLSWL
                     LLVSVPVLAIANYLIVSRMLPIFRRMQKLIDNINRVLREQLSGIRVVRAFAREPVERE
                     RFGEANQAVSDAALAAGRWQALLLPVTTLVINLSSVALIWFGGLRIDAGQMQVGSLIA
                     FLSYFMQILMAVMMATFLLILLPRASVCAERITDVLSTRPTITSPPDPVTAAPKAGLV
                     RLESATFSYPGADRPVLEDISLTARPGSVTAIVGSTGSGKSTLVSLICRLYDVTAGSV
                     TVDGVDVRDYDIETLWSTVGLVPQRGYLFSGTVADNLRYGKADATGAEMWEALRVASA
                     DSFVRAHADGLGMPVAQGGINFSGGQRQRLAIARAVIRRPTIYLFDDAFSALDVHTDA
                     RVRAALRHVSAESTVIVVSQRISTVAEADYIVVVDDGRVVGAGTHEALIAECAQYAEF
                     ADSQAVGVRT"
     gene            4106..4651
                     /locus_tag="VF874_21840"
     CDS             4106..4651
                     /locus_tag="VF874_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEX9178606.1"
                     /translation="MRRRLTSMAIGAATVALVAACSTNPSGPDNPTTSAPPTQQNATH
                     GPFFPECGGVSDQTVSQLTEALGLVNTARNSAGCQWLQNGGIMGPHFSFTWYRGSPIG
                     RERKTEELQRTSVEDINIDGHSGFIAVQTDPTAGDSLCEIGIQFDDDFIEWSISFNEK
                     PFPSPCDVAKELTRQSIANSK"
     gene            4648..5199
                     /locus_tag="VF874_21845"
     CDS             4648..5199
                     /locus_tag="VF874_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEX9178607.1"
                     /translation="MSRARQVLCVVVAALAVAAMLGGCTRTTGGTAVVAGSSDTPRND
                     NSEKQYPNLLKECDILTTDVLAKTVGADPLDIQSTFVGAVCRWQAANPAGLIDITRFW
                     FEQGSLDNERKIADQLKYQVETRSIAGVRSIVMKPNDPNGACGVASDAGGVVGWWVNP
                     QTPGIDACGMAVKLMELTLATNA"
     gene            complement(5223..5723)
                     /locus_tag="VF874_21850"
     CDS             complement(5223..5723)
                     /locus_tag="VF874_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015385265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9178608.1"
                     /translation="MAERTLLLMRHAKSDYPSGVIDHDRPLAPRGVREASLAGDWLRS
                     HVPPIDAVLCSTATRTRETLSHTGLNAPVTFAERLYDAVPGTVIDEINRVGDEVATLL
                     VVGHEPAMSNVALGLADVSGSSHAAAERISTKFPTSAIAVLRFGHLWRDLTRGSAALV
                     TFHVPR"
     gene            5856..7058
                     /locus_tag="VF874_21855"
     CDS             5856..7058
                     /locus_tag="VF874_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEX9178609.1"
                     /translation="MRFIHTADWQLGMTRHFLNGDAQPRYSAARRDAVRALGELAAAT
                     GAEFVVVAGDVFEDNQLDPRVVSQSLEAVRAIGVPVYLLPVNHDPLDASSVYTSALFV
                     TEQPANVRVLDRPGVHEVRPGLQIVAAPWRSKSPTVDLVAGVLDGLPADGVTRILVAH
                     GGVDALDPASGKASLIRLGAVHDALTCGALHYVALGDKHSRTEVGASGRVWYSGSPEV
                     TNYDDVEHDPGHVLVVDIDDDDPARPVTVTAHRVGRWRFVTLRRPVDGSRDIADLDVN
                     LDLLPDKDRTVVRLALTGSLSVTDRAALDACLDKYARLFASLRMWERRSDIAVVAEDA
                     EFADLGIGGFAAAAVEELVATARSDDDAAADAQAALALLLRLKGGDVGAPPACGGPLA
                     RRAKGSAA"
     gene            7055..9673
                     /locus_tag="VF874_21860"
     CDS             7055..9673
                     /locus_tag="VF874_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9178610.1"
                     /translation="MKLHRLVLTNYRGITHREIVLPDSGVIVVSGANEIGKSSMIEAI
                     DLLLEAKDRSAKKEVKQVKPTHADVGAEVTAEISSGPYRFVYRKRFHKHCETALTVLA
                     PQREQLTGDEAHDRVCAILAETVDAGLWRAQRVWQAASTDAVDLSGCDALSRALDVAA
                     GQSAALSGDEPLLIDRIDDEYARYFTPTGRATGEWAAAIARLAAADEEVAGCAAAVAE
                     VDDGVSRHAELSERLAELDTQRESAQQRLTAARAAAAVVAALTAELKEAQVVSGAAQA
                     TADASAAALTERRRLSADVDERVEAVADLEAEAAQVAEHESVAAEVRDAAAAAAERSS
                     AAVDDAQSRADAARARFDRLSDRDEANRLTARLAKIDVACAERDQAGEEIAGNAVTDA
                     VLRDIEVAAAAVDRASSLAETSCPRIELISPTDLQLLVQDRPVALSADQTFTLSATGV
                     TDVEVPGVLRARVVPGAPACDTQATLEAAQRHQDGVLAAAGVADVADARAHAERRREL
                     VSERDRLTATLWGLCGDEPVDELRSRLAALQSRFAQGDDPDMDTSSARAALDAATAMH
                     RDAVAQCQRDREVAAAAEASCAEKRTVAAVLREKLATARTELDAVRQRLVQQRATASD
                     EDLQTCASTDAQTSERATAREADVRARLAETVVQEVSAELGAAERHADEVSRCHDTVG
                     TELQDVTTQLRVYGNEGRKGRLDAAEAEQAHAGSEWTRVGCRARAVQLLRSVILRHRD
                     DTRLRYVEPFRAEVQRLGRIVFGDSFEVEIDSSLRICSRTLAGCTVPYESLSGGAKEQ
                     LGIVARLAGAALVAKEDSVPVIIDDALGFSDSERLAKMGAVFDAVGSDAQVLVLTCSP
                     RRYDSVDGAHHIELTA"
     gene            complement(9678..11057)
                     /locus_tag="VF874_21865"
     CDS             complement(9678..11057)
                     /locus_tag="VF874_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005201249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9178611.1"
                     /translation="MTEFMRNSDAFTWAMESDPRLRSTVVTVILLDRSPDWDEVRNRF
                     GLVCEKVPTLRQRVVASPPPAPPRWEFDPDLDLDFHMRRVIAPPPATFDTVLEMARVA
                     AMEDFDRARPLWEATLIDGLVDGGAAVVLKFHHALTDGVGGIQIGMILFDLSEVPEKH
                     GPVADLPEVPAPPWLSGYRETARYNARLIGNTLVGALKSAPKLIFNGIRRPVQSVSSA
                     AEMTASVYRTVRPVNRTGSPLMMNRTLIRRLGVHEVPFPALREAAHHAGGALNDAFVA
                     GVAGGLRRYHERHGVEVGDLHLTMPMSLREEGDELGGNRITLMRFDVPVGIADPGERI
                     REIHERTASVRHEKSLPYTQWIAGALNLMPRWYIGSILRHVDFLCSDVPGIPVPVFLG
                     GARVRVQYAFGPTVGAAVNVTLLTYVDVSALGIDVDTGAIPDYDVFHECLVAGFDEVL
                     ALATAAASG"
BASE COUNT         1889 a         3820 c         3691 g         1678 t
ORIGIN      
        1 gtcatccgtg ccgcgatgtc ggtgatcgcc tcgtcccagg tggcctccac atacgaatcg
       61 ccgacgcggc gcatcgggtt caggattcgc cgcggatgtt cgaccatctg gttcgccgtg
      121 cggcccttgg cgcagaagtc ctgccagctg tgggggttgt ccttgtcggc ggagatcttg
      181 atcacccgat tgtcctcgac cgtcactgca atgccgcacg ctgccaggca gtatcggcaa
      241 aacgtgtaga cggtctccgg ctcaaccgct gtcattacga cgagactacg tcgctaggcc
      301 tgcgtcatcg cccaataagc gccacgctta gccatcagct cggtgtgact accccgctcg
      361 acgatccgcc cgtcatccat caccaggatc acgtcggcat cgcggatcgt cgaaagccgg
      421 tgggcgataa tgaaactcgt acggtcacga cgcagctcgc tcatcgcatg ctggactagc
      481 acctcggtgc gggtgtccac cgagctggtg gcctcgtcga ggatcagcag ctgcggttgg
      541 gcgagaaatg cccgagcgat cgtgatcaat tgcttctcgc cggcgctgat gttgccgccg
      601 tcgtcgctca ccggagtgtc gtagccctcc ggcagtgtgt gcacgaaccg gtcgacgtac
      661 gcggccctag cagcagccag cacctcgtcg tcgccggcgc cgggacggcc gtaacggatg
      721 ttctcggcga tcgatccacc gaacagccac gtgtcctgca ataccatccc gatgcgtgat
      781 cgcagtgact ggcggctcac cgtcgcgacg tcgacgccgt cgacgaggat ccggccggag
      841 tcgacctcgt agaaccgcat cagcaggttc accagcgtgg tcttgcccgc acccgtcggc
      901 ccgacgatcg ccaccgtgct gccgggatcc gccacgaacg acaggtcttc gatcaccggg
      961 gtgccgcgac ggtaactgaa gttcacccgc tcgaactcga cgcggccgac ccggccgttg
     1021 accgacggca gcggcagtcc ggtgtcgggc gtctgttctt cggtgtcgag caggtcgaaa
     1081 acccgctcag cgcttgccat tccggactgg agcgtgttgt acatcgcggc aacctgggtc
     1141 agcggctggt tgaactggcg cacgtactgg gtgaacgcct ggatgctgcc cagcgtgatc
     1201 tgtcctgtcg ccacctgcag gccgccgacc accgccacgg cgacgtagct gaggttgccg
     1261 atgaacatcg tgaacggcga caccagtccc gaaaagaact gcgcgccaaa gctggcgtcg
     1321 tacaggtcgc cgttgagctc gcggaacctc tgctgagcat gcgcccggtg gccgaacgtc
     1381 ttgaccaggg tgaagccgct atacgtctct tcgatgtggg cgttcagccg cccggtgttg
     1441 gtccactgcg cgacgaacag ccgccgcgat ctgcgtgcga tcgtccgagt cacccacagc
     1501 gacaacggaa cggtcagcac ggtgatcatc gccagtagcg gcgagatcgt cagcatcatc
     1561 accagcacgg cgaccaccgt cagcaccgac gtcaacagct ggctgatcgt catcgacagc
     1621 gacgtctgaa tgttgtcgac gtcgttggtg acccggctca gcacctcacc gcgctgccga
     1681 gaatcgaagt acgacaacgg caaccggtgc accttgtcct cgacatcggc tcgcagcgtc
     1741 accatcgtgc gctgcaccgt gacgttcagc agccggaatt gcgcccaaac cagcactgcc
     1801 gcaagtagat agaggcccag tgccagcagc agggtgcggc cgatcgccgc gaagtccaca
     1861 ccgacgcccg gcgtgacgtt catccgagac aacaggtcgg cgaacgtccc gtcgccgcgg
     1921 gctcgcgcgg cctcgacggc ctgctccttg gtgatgcccg cgggcagctg ccgcccgatg
     1981 accccgttga acagcaggtc ggtcgcatgc ccgaggattc gcggaccgat gacgccgatc
     2041 gcgatgccga cgatacccag tccgatcacg gccgcggtca acccgcgctg cggagtcagt
     2101 cgcttcagaa gccggatcgc ggcaccccgg aaatcgcgcg accgttccgg cgcggacggc
     2161 atcccgccct gcatcatccg catcggcgcg ctcatgtccg cacccctacc gcctgcgagt
     2221 ccgcgaattc cgcgtactga gcgcactcgg caatcagagc ctcgtgggtt cctgcgccga
     2281 ccacgcgtcc gtcgtcgacg acgacgatgt aatcggcctc ggcgacagtc gaaatacgtt
     2341 gcgatacaac gataacggtc gattctgccg acacgtgccg cagtgccgcc cgcacgcgcg
     2401 cgtcggtgtg tacgtcgagc gccgagaacg cgtcgtcgaa caggtagatc gtcggtcggc
     2461 ggatcaccgc gcgcgcgatc gccaggcgct gccgttgccc gccggagaag ttgatgccgc
     2521 cttgcgccac aggcattccc agcccgtcgg cgtgcgcccg cacgaagctg tcggccgacg
     2581 ccacccgcag cgcctcccac atctcggcgc cggtggcgtc ggcctttccg tagcgcaggt
     2641 tgtcggccac ggtgccggag aaaaggtagc cgcgttgcgg caccaatccg accgtcgacc
     2701 acagcgtctc gatgtcgtag tcgcggacgt cgacgccgtc gacggtcacc gaccccgcgg
     2761 tcacgtcgta cagccggcag atcagcgaca ccagcgtcga cttgcccgac ccggtgctcc
     2821 cgacgatcgc ggtcacgctt ccgggccgtg cggtcaacga gatgtcttcc agcaccggcc
     2881 tgtcggcgcc cgggtaggaa aacgtcgccg actccagtcg cacgagcccc gccttcggag
     2941 ccgccgtcac cggatccggg gggctggtga ttgtggggcg ggtcgacaac acgtcggtga
     3001 tccgttcggc gcacaccgac gcccgcggca gcaggatgag aaggaaagtc gccatcatca
     3061 cagccatcag gatctgcatg aaataggaca ggaacgcgat cagcgacccg acctgcatct
     3121 gcccggcgtc gatgcgcaag ccgccgaacc agatcagcgc gacgctggac aggttgatca
     3181 ccagcgtcgt caccggcagc agcagcgcct gccaccgtcc ggcggccagc gccgcgtccg
     3241 acaccgcctg attcgcctcg ccaaatcgct cgcgttctac gggttcgcgg gcgaaggccc
     3301 ggacgacccg gatgcccgac aactgctcgc gcaggacgcg gttgatgttg tcgatgagct
     3361 tctgcatcct gcggaagatg ggcagcatcc gcgacacgat caggtagttg gcgatcgcga
     3421 gcacgggcac gctgaccagc agcagccacg acagcccggc gtcctggtgc agggccatca
     3481 cgattccccc gatacacatg atcggcgcgg tgatcagcat cgtgcacgtc agctgaacca
     3541 gcagctggat ctgctggacg tcgttggtgg tgcgggtcaa cagcgacggg gccccgaagc
     3601 gggcggtctc gtgcgccgag aacccggtga cgtggtcgaa gatcgcccac cgaaggtcac
     3661 gcccgaaccc catcccggtg cgcgagccga aatacaccgc cgctaccgcg cacagcacct
     3721 ggagcgcgct gacggcgagc atcaccccgc ccagccgcat gatcgtctgc gtgtcgccct
     3781 tggcgacccc gtcgtcgatg atcgccgcgt tgaccgtcgg caggtacaac gccgcgagcg
     3841 tgctgattac ctgcagcgcc gcgacgatcc cgacgagcga gcggtacggc cgcacatatt
     3901 cgcgcagcag tcctgcgagc atctccgtac tgtcgcacac cgcgcgcgca gttcggcacc
     3961 ccgacgcacc gcgcggcggg cgcaaaaccg ctggtcaacg ctgggatcgc gcctgggtcc
     4021 tgcggctgaa gctatcctgc ttgctgtgac gagcggcagg gatgcgggtt cgtcggcgca
     4081 atcggcgaaa aaggaagcgg gttcggtgag acgcagactg acctccatgg ccatcggcgc
     4141 agcgaccgtt gcgctggtcg ccgcgtgctc caccaacccg tccggtccgg acaacccgac
     4201 cacgtcggcg ccgcccaccc agcagaacgc cacccacggg ccgttctttc ccgaatgcgg
     4261 cggggtcagc gaccaaaccg ttagccaact gaccgaagcg ctcgggctcg tcaacaccgc
     4321 acgcaactcc gccggctgcc aatggctgca gaacggcggc atcatgggcc cgcacttctc
     4381 gttcacctgg taccggggca gtccgatcgg tcgggaacgt aagacagagg agctgcagcg
     4441 aaccagcgtc gaggacatca acatcgacgg ccacagcgga tttatcgcgg ttcagaccga
     4501 cccgaccgct ggcgacagcc tgtgcgagat cggcatccag ttcgacgacg acttcatcga
     4561 atggtcgatc agtttcaacg agaagccctt cccgtcgccg tgcgacgtcg ccaaggaact
     4621 cacccgccag tcgattgcga attcgaaatg agccgggccc gtcaagtgct gtgcgtggtt
     4681 gtcgccgcgc tcgcggtcgc cgcgatgctg ggcggctgca cacgcaccac cggaggcacc
     4741 gccgtggtgg ccgggtcgtc ggacacgccg cgcaacgaca attccgagaa gcagtacccg
     4801 aacctgctca aggaatgcga catcctgacc accgatgtgt tggccaagac cgtcggcgcg
     4861 gatccgcttg acattcagag cacattcgtc ggtgccgtct gccggtggca ggcggccaac
     4921 ccggccggcc tgatcgacat cacccgcttc tggttcgagc agggcagcct cgacaacgag
     4981 cgcaagatcg ccgaccagct gaagtatcag gtcgagaccc gctcgattgc gggcgtccgc
     5041 tcgatcgtga tgaaacccaa cgaccccaac ggcgcatgcg gcgtggccag cgatgccggt
     5101 ggagtggtcg gctggtgggt gaaccctcag acgccgggga tcgacgcgtg cgggatggcg
     5161 gtcaagctga tggagctgac gctcgccacc aatgcttgac gcggacgcga ggagcgaaaa
     5221 aactaccgcg ggacgtggaa tgtgaccagc gccgcgctac cccgggtgag gtcgcgccac
     5281 aaatggccaa aacgcaacac ggcaatcgcc gacgtcggga acttcgtcga gatacgttcg
     5341 gccgcggcgt gactgcttcc tgagacgtcc gccaatccga gcgcgacatt gctcatcgcc
     5401 ggttcatgcc ccaccaccag cagcgtggcg acctcatcgc cgacgcggtt gatctcgtcg
     5461 atcaccgttc ccggcaccgc gtcgtacagc cgttcggcga acgtgaccgg cgcgttaagc
     5521 ccggtgtggg ataacgtctc gcgcgtgcgg gtggccgtcg agcacagcac cgcgtcgatg
     5581 ggcggcacgt gcgatcgcag ccagtcgccg gcgagactgg cctcccgcac gccgcgtggt
     5641 gccagcggcc ggtcgtggtc gatcaccccg ctcgggtaat ccgacttcgc gtgccgcatc
     5701 agcaacaagg tgcgctcggc catccaccca cgctaaggga cgatgagccg cttgcgcgaa
     5761 gagcatgacc gcgccgcgcg aggtccacga acttgtcggc ggccggtcct acactcgcct
     5821 tgcttcgaga accgacgccc gaaaggaggg ccgacatgcg attcatccac accgccgact
     5881 ggcagctcgg catgacccgg cacttcctca acggcgatgc acagccgcgc tattcggcgg
     5941 cccgccgcga cgcggtccgc gcgctcggcg agttggccgc tgcgacgggc gcggagttcg
     6001 tcgtggtcgc gggggacgtc ttcgaggaca accaactcga cccgcgggtc gtcagccagt
     6061 cactggaggc cgtgcgcgcc atcggtgtcc cggtctacct gctgcctgtc aaccacgacc
     6121 cgctggacgc gtcgtcggtc tacaccagtg cgctgttcgt caccgagcag ccggcgaatg
     6181 tcagggtgct cgaccggccc ggtgtgcacg aggtgaggcc ggggctgcag atcgtcgccg
     6241 cgccgtggcg gtcgaaatcg cccaccgtcg accttgtcgc cggcgttctc gacggcctgc
     6301 ctgcggacgg ggtgacccgg atcctcgtgg cgcacggcgg ggtcgacgcg ctcgaccccg
     6361 cttcgggcaa ggcgtcgttg attcggctgg gcgcagtgca cgacgcgctg acgtgcggtg
     6421 cgctgcacta tgtcgcgttg ggggacaagc attctcgtac cgaggtcggc gccagtggcc
     6481 gggtgtggta ctcggggtca ccggaggtca ccaactacga cgacgtggag cacgatccgg
     6541 ggcacgtgct ggtcgtcgac atcgacgacg acgatcccgc acggccggtg accgtcaccg
     6601 cgcatcgcgt cggcaggtgg cggttcgtga cgctgcgccg cccggtggac ggcagccgcg
     6661 acatcgccga cctcgacgtc aacctcgacc tgttgcccga caaggaccgc acggtggtgc
     6721 ggttggcgct gacgggctcg ctcagtgtca ccgaccgggc ggcgctcgac gcgtgcctcg
     6781 acaagtacgc gaggctgttt gcgtcgttgc ggatgtggga gcggcgcagc gacatcgcgg
     6841 tagtcgcaga agacgccgag ttcgccgatc ttggcatcgg cggctttgcg gcggcggccg
     6901 tcgaggagtt ggtcgccacc gcccggtcgg acgacgacgc cgcggccgac gcgcaggcgg
     6961 cgctggcgct gctgctgcgg ctcaagggcg gcgatgtggg ggcacctccc gcttgcgggg
     7021 gaccgcttgc gcgaagagca aaaggcagtg cggcatgaag ctgcaccggc tggtcctcac
     7081 caactaccgc ggcatcacgc accgcgagat cgtgctgccc gacagcggcg tgatcgtggt
     7141 cagcggcgcc aacgaaatcg gcaagtcctc gatgatcgag gccatcgacc ttctgctgga
     7201 agccaaggac cgctccgcca agaaggaagt caaacaggtc aagcccactc acgccgacgt
     7261 cggcgccgag gtgaccgccg aaatcagtag cggcccttat cggttcgtct atcgcaagcg
     7321 tttccacaag cattgcgaga ccgcgctgac cgtgctcgct ccgcagcgcg aacagctcac
     7381 cggcgacgag gcgcacgacc gggtctgcgc gatcctggcc gagaccgtcg acgccggact
     7441 gtggcgggct caacgggtgt ggcaagcggc gtccactgac gccgtcgact tgtcgggctg
     7501 cgatgcgcta tcgcgtgcgc tcgacgtggc cgccggccag tcggctgccc tctcgggtga
     7561 cgagcccctg ttgatcgacc gcatcgacga cgagtacgcc cggtacttca cccccaccgg
     7621 gcgcgcgaca ggggagtggg cggcggcgat cgcccgcctc gccgcggccg acgaagaggt
     7681 ggcaggctgc gccgcggccg tggccgaggt cgacgacggt gtgagccggc atgccgagct
     7741 gtcggagcgg ctggccgagc tggacacgca acgcgagtcg gcgcagcagc ggctgaccgc
     7801 cgcccgcgcg gcagccgccg tcgtcgccgc actcaccgcc gaattgaagg aggcgcaggt
     7861 cgtttccggg gcagcgcagg caacggccga cgcgtctgcg gcggcgctga ccgaacgacg
     7921 acggttgagc gccgacgtcg acgagcgcgt cgaggcggtc gccgacctgg aggccgaggc
     7981 ggcacaggtc gctgagcacg agtcggtcgc agccgaagtt cgcgacgccg ccgcggcggc
     8041 ggccgagcga tccagcgccg cggtggacga cgcgcagtcc cgcgcggacg cggctcgcgc
     8101 gaggttcgac cggttgtccg atcgtgacga ggccaaccga ctgacggcca ggctcgccaa
     8161 gatcgacgtc gcgtgcgccg aacgggacca ggccggggag gagatcgcag ggaacgcggt
     8221 gacggatgcg gtgctgcgag atatcgaggt ggcggccgcg gcggtggacc gggcgtcgag
     8281 tctcgccgag acctcctgcc cgcgaataga actcatctcg cctaccgacc tgcagctgct
     8341 ggtgcaggac cgcccggtcg cgctgtcggc ggaccagacg ttcaccctct ccgccaccgg
     8401 ggtcaccgac gtggaggtgc ccggcgtact gcgcgcacgc gtcgttcccg gcgcgcccgc
     8461 atgcgacacc caagccacgc tcgaagcggc gcaacgccat caggacggtg tgcttgccgc
     8521 cgccggcgtc gcggatgtgg ccgatgcgcg cgcccatgcc gaacggcgcc gcgaactggt
     8581 ctccgagcgt gaccggctga cggcgacgct gtggggcctg tgcggtgacg aaccagtgga
     8641 cgaactgcgt tccaggctcg cagcgttgca gagtcggttc gcccagggcg acgacccaga
     8701 catggacacg agttccgccc gggccgcact cgacgccgcg accgcaatgc atcgcgacgc
     8761 cgtcgctcaa tgtcagcgcg accgggaggt cgccgcggcc gccgaagcta gctgcgctga
     8821 aaagcgcact gtcgcagccg ttctgcggga aaagctggcc accgcgcgca ccgagcttga
     8881 cgccgttcgg cagcggctcg tccagcagcg cgcgacggcc agcgacgagg atcttcagac
     8941 ctgcgcgagt accgatgcgc agacgtccga acgtgccacg gcaagagagg ccgatgttcg
     9001 ggcccgattg gccgagaccg tcgtgcaaga agtcagcgcc gaactcggcg ccgccgagcg
     9061 tcacgccgac gaggtttctc gttgtcacga cactgtcggc accgaattac aggacgtcac
     9121 aacgcaattg cgcgtgtacg gcaacgaggg ccgcaagggc cggttggacg cggcggaggc
     9181 ggaacaggcg catgctggca gcgagtggac ccgtgtgggt tgtcgagcgc gcgccgtcca
     9241 gctgctgcgc tccgtgattc tgcggcaccg cgacgacacc cggctgcgtt acgtcgagcc
     9301 gttccgggcc gaggtgcagc ggctcggccg gatcgtgttc ggcgacagct tcgaggtcga
     9361 gatcgacagc tcgctgcgga tctgcagtcg caccctggcc gggtgcaccg tgccctacga
     9421 gtcgctgtcc ggcggcgcga aggagcagct gggcatcgtg gcccggcttg cgggggccgc
     9481 gctggtcgcg aaagaggaca gcgtgccggt gatcatcgac gacgcgctcg gcttcagcga
     9541 ctccgagcgg ctggccaaga tgggcgcggt gttcgacgcc gtcggcagcg acgcgcaggt
     9601 gctcgtgctg acctgcagcc cccgccgcta cgacagcgtc gacggcgccc accacatcga
     9661 attgaccgcg tgacgcgtca accggacgcg gcagccgtgg cgagcgccag cacctcgtcg
     9721 aaaccggcga ccaagcattc gtggaacacg tcgtagtcgg gaatcgcgcc ggtgtccacg
     9781 tcgatgccca gcgcactgac gtccacgtac gtcagcagcg tgacattcac cgccgcaccg
     9841 accgtcggcc cgaacgcgta ctggacccgc acccgggcgc caccgaggaa caccggcacc
     9901 ggaatccccg gcacgtcact gcacaagaag tcgacgtggc gcaggatcga gccgatgtac
     9961 caccgcggca tcaggttcag cgcgccggcg atccactgcg tgtagggcag cgacttctcg
    10021 tggcggacgc tggctgtccg ctcgtggatc tcgcggattc gttcgccggg atccgcgatg
    10081 ccgaccggca catcgaaccg catcaacgtg atccggttgc cccccaattc gtcgccctct
    10141 tcccgcaggc tcatcggcat cgtcaggtga aggtcgccga cctcgacacc gtgtcgttcg
    10201 tggtagcggc gcagcccgcc ggccacgccc gcgacgaacg cgtcgttcaa cgcaccgccg
    10261 gcatggtggg cggcctcccg gagcgccgga aacggcacct cgtgcacgcc gagacgccgg
    10321 atcagggtgc ggttcatcat cagcggcgaa cccgtccggt tcactgggcg cacggtccgg
    10381 tagaccgacg cggtcatctc ggcggccgac gagacggact gcaccggccg ccggatgccg
    10441 ttgaagatca gcttcggggc gctcttcaat gcgcctacca gcgtgttgcc gatcaggcgc
    10501 gcgttgtatc gcgcggtctc gcggtagccg ctcagccacg gcggcgcggg cacctcgggc
    10561 aggtccgcca ccggtccgtg cttctccggg acctcggaca ggtcgaacag gatcatcccg
    10621 atctggatcc cgccgacgcc gtcggtgagc gcgtggtgga acttgaggac cacggccgcg
    10681 ccaccatcca ccaagccgtc gatcagggtg gcctcccaca gcgggcgggc cctgtcgaag
    10741 tcctccatcg ccgcgactcg ggccatttcc agcaccgtgt cgaacgttgc gggcgggggc
    10801 gcgatcactc tgcgcatgtg gaagtcgagg tctaaatcag ggtcgaactc ccaccgcggc
    10861 ggcgccggcg gcggcgatgc caccacccgc tgccgcagcg tcggcacctt ctcgcagact
    10921 aggccgaacc ggttgcgcac ttcatcccaa tccggagacc ggtccagcag gatcaccgtg
    10981 accaccgtgg aacgcagtcg cggatcgctt tccatcgccc acgtgaaggc gtcgctgttt
    11041 cgcatgaatt cagtcatttg tgccaaacct acgacgcc
//
LOCUS       DASWBF010000584         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1111997,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000584 DASWBF010000000
VERSION     DASWBF010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111997"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VF874_21870"
     CDS             complement(<1..1103)
                     /locus_tag="VF874_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007322550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX9178612.1"
                     /translation="MDAVDEQLIRQLADRARAEGLQLTGEGGLLTRLTKTVIESALEG
                     ELDDHLGYAKHDPAGRNGENSRNGRRGKSVLTEVGPVEIAVPRDRDGSFEPRLVAKHQ
                     RRLSGVEDLVISLSAKGLTTGEIAAHLAEVYGAQVSKQTISAITDRVLEGMAEWQSRP
                     LDAVYPVLFIDCVNVKIRDGNVANRPIYVALAVTVTGTRDILGLWAGEHGDGEGAKYW
                     LRVLSEIKNRGVADVCLVVCDGLKGLPEAIETVWPQAITQTCVVHLLRNSFRYASKRD
                     WSAIAKDLKPVYTAPSEQAALEAFLTFTETWEQRYPAIIKLWDNAWPEFVPFLRFDTE
                     IRTVVCTTNAIESINARIRRAVNARGHFPTEQAA"
     gene            1248..>1558
                     /locus_tag="VF874_21875"
     CDS             1248..>1558
                     /locus_tag="VF874_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9178613.1"
                     /translation="MARLADPSGGPGIQVRLLGPFEVVLDDVAQRLPGRGERALLALL
                     ALSAERMVAASSLVRQLWAPADLPDDPLNALQIRVSKLRRALAGMGCRDRVLRDGAGY
                     RL"
BASE COUNT          228 a          550 c          499 g          281 t
ORIGIN      
        1 gcggcctgct cggtggggaa gtgaccgcgg gcgttgacgg ctctgcggat gcgggcgttg
       61 atcgactcga tggcgttggt cgtgcacacc acggtgcgaa tctcggtgtc gaaccgcagg
      121 aacggcacga actccggcca cgcgttgtcc cagagcttga tgatcgcggg gtagcgctgc
      181 tcccacgtct cggtgaaggt caagaacgcc tccagggcgg cctgctccga cggcgcggtg
      241 tagaccggtt tgagatcctt ggcgatcgcc gaccagtccc gcttcgatgc ataacgaaac
      301 gaattccgca gcaggtggac aacgcacgtc tgggtgatcg cttgtggcca cacggtctcg
      361 atcgcctccg gcagaccctt gagcccgtcg cagaccacca ggcacacgtc ggcgacgccg
      421 cggtttttta tctcggacag cacccgcagc cagtacttgg cgccctcccc gtcgccgtgc
      481 tcaccggccc acagcccgag aatgtctcgg gtgccggtga cggtgaccgc tagcgccacg
      541 tagatcggcc ggttggccac gttcccgtcg cggatcttga cgttgacaca gtcgatgaac
      601 aacaccggat acaccgcgtc caacggccgg gactgccact cggccatccc ctccagcacc
      661 cggtcggtga tcgccgagat ggtctgcttg gacacctgcg cgccgtacac ctcggccagg
      721 tgcgcggcga tctccccggt ggtcaacccc ttcgccgaca gcgagatcac caggtcctcc
      781 accccggaca gccgccgctg atgcttggcc accagcctcg gctcgaagct gccgtcccgg
      841 tcccggggca ccgcgatctc caccggcccc acctcggtga gcacgctctt gccgcgccgc
      901 ccgttgcggg agttctcccc gttgcggccg gccgggtcgt gcttggcgta gccgagatga
      961 tcgtcgagct cgccctccaa cgccgactcg atcaccgtct tggtcagccg ggtcagcagc
     1021 ccaccctccc cggtcagctg cagcccctcg gcgcgggccc gatcggccag ctgccggatc
     1081 agctgctcat cgaccgcatc cagcgcatcc tccccggccg gcgtactcac agtcacatca
     1141 gtcacaggtg cctcctacgg ctcaggagtt acaccaactt tcttacactc cctcttcggc
     1201 tgctgccgtt ctttgtccta ttggcggcgg actgggagga tcgcggtgtg gcccggttgg
     1261 cggacccgtc aggtggtccg ggaatccagg tgcgactgct cggaccattt gaggttgttc
     1321 tcgacgacgt cgcgcagcgt ctaccgggcc ggggcgagcg tgccctgctg gcgctgttgg
     1381 ctttgtccgc ggagcggatg gtcgccgcca gttcactggt tcgacagttg tgggcgccgg
     1441 ccgatctgcc ggacgatccg ctcaacgcac tgcagatccg cgtgtcgaaa ctgcggcgcg
     1501 cgctggccgg catgggttgc cgcgaccgag tgctgcgaga cggggccggg taccggct
//
LOCUS       DASWBF010000585         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_920120,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000585 DASWBF010000000
VERSION     DASWBF010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920120"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VF874_21880"
     CDS             <1..134
                     /locus_tag="VF874_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9178614.1"
                     /translation="IELGNMKNQPEAAVMTTAEGRAKYAAAVTQGIAAYLSQPRGTA"
     gene            415..2139
                     /locus_tag="VF874_21885"
     CDS             415..2139
                     /locus_tag="VF874_21885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178615.1"
                     /translation="MRSGAEYFTQPGGAGQRRYEALRAYFVEEASAAEIADRFGYSTA
                     SVHQMATLLRTGKLAPFTEAKPGPKGPRKATGQVREKVLALRAGGRSVGEIAESLTEV
                     GTPISAQTVWQILDDAGLPRLDRRDETRRGSPAKLDPVKAAAQPSWPAGATIGCGHAG
                     LLLLFPAMVELGLHDLVEQAGYPSTAAITAWQSIGTLLLAKCARRHRAHHIDALTDDA
                     GLAHALGLTALPKATHAGSYSYRVRRESNQKLLAGLVKALRGLGMASGAEGFNLDFHA
                     IRHHGADTVLEKHYVPRRSQRTRAVLTFFAQDHASAEMVYANAEITKAEQAREILAFA
                     DYWHAATGADPGLLVFDSQLTTYKVLNELTHRGIGWLTLRQRGSTEIARLTALPASRW
                     KTVTIARRGRYRRPQLHEDMIKLNDVDTKVRQIAVRNIGRDQPTLLISNDLTTPAKDL
                     FARYAERMSIENELDAYISGYHLDALSSGVPLNVDLDTTLTVLAGNLYRLFARDLTRY
                     ETATPDTIWRHFLDDNGTLHITDQGVTCALNLRAHHPQLIDAGYADITTPIPWWNNRT
                     LQFNFPPR"
     gene            complement(2223..>2457)
                     /locus_tag="VF874_21890"
     CDS             complement(2223..>2457)
                     /locus_tag="VF874_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178616.1"
                     /translation="FRGAFAYVTARLPDGDTMPLMRLRYGGSAARWGIRGVPGQQERL
                     PRLGPADRPHGRRTEDALDTACGLYLGDPTAWT"
BASE COUNT          464 a          931 c          708 g          354 t
ORIGIN      
        1 tcatcgagtt gggcaacatg aaaaaccagc cggaggcggc ggtgatgacc accgccgagg
       61 gcagggccaa atacgcggcc gcagtaacgc aaggcatcgc ggcctacttg tcacagccac
      121 gaggcaccgc ctaacctcga ttcacgggag cgggttagct ccactcctcc ggagctgacc
      181 gggagcagat tcttgcatcg ccgctggtag gcggcttgac caggcggcag acctcaattg
      241 atttaccgga acagggcgcg ccgatctgct gaggctgtat tcggacatcc ggtgctcgtc
      301 gtatcgactt acattcgtgt aattcgtgct cgcccatggg tgcccaccgg cgtggcgctc
      361 tgccggaaag gtaatacagc tctagcatag gtgcatgtcg ttcagggaaa ccagttgagg
      421 tctggggcgg agtatttcac ccagcccggc ggcgcggggc agcgccgcta tgaggcgttg
      481 cgcgcctact tcgtcgagga ggcctccgcc gccgagatcg ccgaccggtt cggctactcc
      541 accgcctcgg tgcaccagat ggccaccctg ctgcgcaccg gcaagctcgc cccgtttacc
      601 gaggccaagc ccggcccgaa aggaccgcgc aaggccaccg ggcaggtgcg ggagaaggtg
      661 ctcgcgctgc gcgcgggcgg gcgcagcgtc ggcgagatcg ccgaatcact caccgaggtg
      721 ggcacaccca tctcggcgca gacggtgtgg cagatcctcg acgacgcggg acttcctcgg
      781 ctggaccgcc gcgacgagac ccgccgcggc agcccggcga aactggatcc ggtcaaggct
      841 gccgcgcaac cgagctggcc ggccggcgcg acgatcggct gcggccacgc cgggctgctg
      901 ctgttgttcc cggccatggt cgaactcggg ctgcacgacc tcgtcgagca ggccggctac
      961 ccgtccactg ccgcgatcac cgcctggcag tcgatcggca cgctgctttt ggccaaatgc
     1021 gcgcgccgcc accgcgccca ccacatcgac gcgctcaccg acgacgccgg gctcgcacac
     1081 gcgctcgggt tgaccgcact gcccaaggcc acccacgccg gctcctactc ctaccgggtc
     1141 cgccgcgaat ccaaccagaa actgctcgcc ggcctggtca aggccctacg cggcctcgga
     1201 atggccagcg gtgcagaagg attcaacctc gacttccatg ccatccgcca ccacggcgcc
     1261 gacaccgtgc tggagaaaca ctacgtaccg cgccgctccc agcgcacccg cgccgtgctc
     1321 accttcttcg cccaagacca cgcctcggcc gagatggtct acgccaatgc cgagatcacc
     1381 aaggccgaac aggcccgtga gatcctcgcc ttcgccgact actggcacgc cgccaccggc
     1441 gcagaccccg gactgctggt gttcgactcc cagctgacca cctacaaggt gctcaacgaa
     1501 ctgacgcacc gcggcatcgg ctggctcacc ctacgccaac gcggcagcac cgaaatcgcc
     1561 cgcctcacag cacttcccgc cagccgatgg aagaccgtca ccatcgcccg ccgcggccgc
     1621 taccggcgcc cgcaactgca cgaagacatg atcaaactca acgacgtcga caccaaggtc
     1681 cgccagatcg ccgtgcgcaa catcggccgc gaccaaccca ccctgctcat cagcaacgac
     1741 ctcaccaccc ctgccaaaga cctgttcgcc cgctacgccg aacgcatgag catcgaaaac
     1801 gaactcgacg cctacatcag cggctaccac ctcgacgccc tatcttcggg cgtcccgctc
     1861 aacgtcgacc tcgataccac cctcaccgtc ctcgccggca acctctaccg gctgttcgcc
     1921 cgcgacctca cccgctacga aaccgccacc cccgacacca tctggcgcca cttcctcgac
     1981 gacaacggca ccctgcacat caccgaccaa ggcgtcacct gcgcactcaa cctccgcgcc
     2041 caccaccccc aactcatcga cgccggctac gccgacatca cgacccccat cccctggtgg
     2101 aacaaccgaa ccctccaatt caacttccca cctcgataac ccccaaccgg gcgcaaaacc
     2161 tcaactcaat taccgcaccg ggaatcgagg ctaagtggtt gcgctcgtaa gttcgtcggg
     2221 ggtcaggtcc aagcggttgg gtcgcccagg tagaggccgc aggcggtgtc gagagcgtct
     2281 tcggtgcgcc ggccgtgtgg ccggtcggca ggaccgagtc ttggtagccg ttcttgctgg
     2341 ccaggtacac cgcgaatccc ccagcgggcc gccgatccgc cgtagcgcag ccgcatcagc
     2401 ggcatggtgt cgccgtcggg aaggcgcgcg gtgacgtagg cgaacgcgcc gcggaat
//
LOCUS       DASWBF010000586         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_578523,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000586 DASWBF010000000
VERSION     DASWBF010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578523"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VF874_21895"
     CDS             complement(<1..805)
                     /locus_tag="VF874_21895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HEX9178617.1"
                     /translation="MVAIGAGLLGVVLALATPLLPVKQTTAQLNWPQNGVFSSVTAPL
                     ISYVATDLTITVPCRAAAGLAGPNTTKTALLSTVPKQAPKAIDRGLLIQRVGENLLVI
                     VRNTPVVTAPMSQVLSPACRQLTFTAHADKVAAEFVGLVKGPDSDDPGAPLKGVRDGY
                     DFRPQIVGVFTDLSGPAPPGLSCSATIDTRYSSGPTLIKWIAMILGVAMTVVSLLALH
                     VLDTADGMRHRRFLPARGWSATPLDIVVTAVLVWWHFVGANTADDGYILT"
     gene            complement(947..2827)
                     /locus_tag="VF874_21900"
     CDS             complement(947..2827)
                     /locus_tag="VF874_21900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactan 5-O-arabinofuranosyltransferase"
                     /protein_id="HEX9178618.1"
                     /translation="MRTALTTVGHMLIAAVVAVVVAVVGLAAISRVDWPAYNSSNQLH
                     ALTTVGQFSCLAGLLTAGWLWRRGRRRVAHAAALVFLAAFTVVTLGMPLGATKLYLFG
                     ISVDQQFRTEYLTRLTDTAALRDMTYSGLPPYYPPGWFWIGGRAAALTGTPAWEMFKP
                     WAITSIAVAVVVAFVLWSRMIRFELALIVTTATAATTLAYSTAEPYAAVITVLVPPVL
                     VLAWSGLRGGDRSGGWAAIVAVGAFLGIAALFYTLLVAYTAFTVVIMAVVAAAASRKW
                     DPVLRLVVIALIATAIALIGWLPYLLAAVDGSPSSSGSAQHYLPADGAQLSFPMLQFT
                     LLGALCMIGTIWLVVRARSSTRAAALAIGVLAVYAWSLLSMLTTLLRTTLLSFRLHPT
                     LTVLLATAGAFGFVEGALAIAHRGREERNSRSYEPPTSRRIVAAAGVLGAVGALAFSQ
                     DIPNVLKPDIVVAYTDTDGNGQRADRRPPGAEKYYAEIDAKIRDVTGKARNEVVVLTA
                     DYSFLSYYPYWGFQGLTSHYANPLAQFDKRAAAIESWADLKTADQFVAALDALPWEPP
                     TVFLMRPGANDTYTLRLAEDVYPNQPNVKRYQVALEAALFKDPRFSVTTIGPFVLAIR
                     EY"
     gene            complement(2828..>2907)
                     /locus_tag="VF874_21905"
     CDS             complement(2828..>2907)
                     /locus_tag="VF874_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178619.1"
                     /translation="APPTFRYVMMVLRHIPRPVFRKLPI"
BASE COUNT          500 a          992 c          997 g          418 t
ORIGIN      
        1 tcgtcaggat gtagccgtcg tcggcggtat tcgcgccgac gaagtgccac cacaccagca
       61 ccgcggtgac cacgatgtcc agtggcgtgg ccgaccaccc gcgggccggc aggaaccggc
      121 ggtgccgcat cccgtcggcg gtgtccagca cgtgcagggc gagcagcgac accaccgtca
      181 tcgcgacgcc caggatcatc gcgatccact tgatcagcgt cggcccgctg ctgtaccgcg
      241 tgtcgatggt ggccgaacag ctcaagccgg gcggcgcggg accggacagg tcggtgaaca
      301 cgccgacgat ctgcgggcgg aagtcgtaac cgtcgcgcac ccctttgagc ggcgcgccgg
      361 ggtcgtcgct gtccggcccc ttcaccaggc cgacgaactc ggcggcgacc ttgtcggcgt
      421 gcgcagtgaa cgtcagctgt cggcaggccg ggctgaggac ttggctcatc ggtgcggtga
      481 cgacgggtgt gttgcggacg atcacaagga ggttctcgcc gacgcgctgg atcagcagcc
      541 cgcggtcgat ggccttcggc gcctgcttgg gcacagtgga cagcagcgcc gtcttggtcg
      601 tgttcgggcc cgccaatccg gccgcggcgc ggcacggcac cgtgatggtc aagtccgtgg
      661 ccacgtagga gatcagcggc gcggtgacgg acgagaagac gccgttctgc ggccagttca
      721 actgtgcggt ggtctgcttg accggaagca gtggagtggc cagcgccagc accactccca
      781 gcaggccggc gccgatcgcg accatccgcg cggttcggta gttgcccgcg ccacctgcca
      841 cgtcggtaga tcgtagtggc cgcgacttca ggccccaccg cctccgcgaa ttctgcatca
      901 gggtcgtgat tcttgcccaa ccacagccct ggtgcagaaa tcagcgtcag tactcgcgga
      961 ttgccagcac gaacgggccg atcgtggtga cgctgaaccg cgggtccttg aacagcgcgg
     1021 cctccagtgc gacctgataa cgcttcacgt tcggctggtt cgggtagacg tcttcggcca
     1081 ggcgcagtgt gtaggtgtcg ttggcgccgg ggcgcatcaa gaagaccgtg ggcggttccc
     1141 acggcagcgc gtccagcgcc gcgacgaact ggtcggcggt cttgaggtcg gcccagctct
     1201 cgatcgccgc ggcccgtttg tcgaactgcg ccaacgggtt tgcgtagtgc gacgtcagcc
     1261 cctggaagcc ccagtacggg tagtacgaca ggaagctgta gtcggccgtg agtacgacga
     1321 cttcgttgcg cgccttaccg gtgacgtcgc ggatcttcgc gtcgatctcg gcgtagtact
     1381 tctcggcgcc cggtggtcgg cggtctgcgc gctggccgtt gccgtcggtg tcggtgtagg
     1441 cgacgacgat gtcgggcttg agcacgttgg ggatgtcctg gctgaacgcc agcgcgccca
     1501 cggcccccag caccccggcg gcggcgacga tccgccgcga cgtcggcggc tcgtagctcc
     1561 ggctgtttcg ctcctcgcgt ccgcggtgcg cgatcgccaa cgcgccctcg acgaagccga
     1621 acgctcccgc ggtggccagt agcacggtca gcgtcgggtg cagccggaac gacagcagcg
     1681 tggtgcgtag cagcgtcgtc aacatcgaca gcagcgacca ggcgtagacc gcgagcacgc
     1741 cgatcgccag cgccgccgcc cgcgttgacg accgggcgcg cacgaccagc cagatcgtgc
     1801 cgatcatgca cagcgcgccc agcagcgtga actgcagcat cgggaaggaa agctgcgcgc
     1861 cgtcggccgg cagatagtgt tgcgcgctac ccgagctgga cggcgagccg tcgactgcag
     1921 ccaacagata cggcagccac ccgatcaaag cgatggcggt tgcgatcaga gcaatcacca
     1981 ccagccgcag caccgggtcc cacttccggg aagcagcggc cgccaccacc gccatgatca
     2041 cgaccgtgaa cgcggtgtag gccaccagca gcgtgtagaa cagcgcggcg atcccgagga
     2101 acgcgccgac ggcgacgatc gccgcccaac cgccgctgcg gtcaccgccg cgcaggcccg
     2161 accacgccag caccaacacc ggcggcacca gcaccgtgat gaccgccgcg tacggctccg
     2221 cggttgagta ggccagcgtc gtcgccgccg tcgcggtcgt cacgatcagc gccagctcga
     2281 agcgaatcat cctcgaccac aacacgaatg cgacgaccac cgcgacagcg atcgaggtga
     2341 tcgcccacgg cttgaacatt tcccacgccg gcgtgccggt cagcgccgcg gcccggccgc
     2401 cgatccagaa ccagccgggc gggtagtacg gcgggagacc ggagtacgtc atgtcgcgca
     2461 gcgcggcggt gtcggtcagc cgcgtcaggt attcggtgcg gaactgctga tcgaccgaga
     2521 tgccgaacag gtagagcttg gtggcgccca gcggcatgcc cagggtcacc acagtgaagg
     2581 cggccaagaa caccagtgcg gcagcgtgcg caactcggcg gcggccccgc cgccacagcc
     2641 agcccgcggt cagtagcccg gcgagacaac tgaattggcc gaccgtggtc agcgcatgca
     2701 gctgattgga cgagttgtac gcgggccaat cgacgcggga aatcgcggcc agccccacca
     2761 cggcgaccac gaccgcgaca acggccgcaa tcagcatgtg gccgaccgtg gttagggcgg
     2821 tccgcatcta gatgggcagt ttccggaaca ccggccgcgg aatgtgccgc agcaccatca
     2881 tcacgtagcg gaacgtcggc ggcgccc
//
LOCUS       DASWBF010000587         4060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_651363,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000587 DASWBF010000000
VERSION     DASWBF010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651363"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..569
                     /locus_tag="VF874_21910"
     CDS             345..569
                     /locus_tag="VF874_21910"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9178620.1"
                     /translation="MFENLTWTHLAIIAAVGLLVFGPDRLPGAIRRSAAALRQLRAQL
                     AEPPAGCSSSSDPSSTSCASRWPSCNGYAA"
     gene            745..1017
                     /locus_tag="VF874_21915"
     CDS             745..1017
                     /locus_tag="VF874_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178621.1"
                     /translation="MAQKAVAKSTTRTTAERIRDLDKVEVTVPVLGQIRIPRPERLAF
                     YGGLAALAALQLIDWPIAAALAVGHLLSEDHHSRVAQELGAALEEA"
     gene            complement(1370..1705)
                     /locus_tag="VF874_21920"
     CDS             complement(1370..1705)
                     /locus_tag="VF874_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178622.1"
                     /translation="MTPGRRLRADLDKALEHASRQLGYAVEWTEAELQVIAMAADMAD
                     RAEVLKEMFREETTASTRVKIATELRATQRSVVELVARVHPGDGRAKSERHVRAVSMR
                     WDRRRAADG"
     gene            complement(2104..2571)
                     /locus_tag="VF874_21925"
     CDS             complement(2104..2571)
                     /locus_tag="VF874_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010842938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX9178623.1"
                     /translation="MVSKSRQTTARRAPVQLAAFVVGAVFLLVGVLGFIPGITTNYDM
                     MTFAGHHSEAKLLGLFDISVLHSIVHLAFGVAGLALARTFYGARWFLIGSGTIYVVLW
                     VYGLIVADDGAANFVPVNSADNWLHLGLGVGMLLLGLVLGRRRAQPLAMPLPK"
     gene            complement(2752..3774)
                     /locus_tag="VF874_21930"
     CDS             complement(2752..3774)
                     /locus_tag="VF874_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX9178624.1"
                     /translation="MRLRRSNVGGPGVRRVRRGRGFSYAEAGGTPLTDPDTLARIEEL
                     VIPPAWKNVWICPYPNGHIQAVGTDAAGRKQYLYHQRWQQERAEEKYDRTLSLAKRLP
                     DWRTQVVSDLRGRGLQCARVLAVAQRLLDRGYFRAGGEEYAQENGSFGLATLRREHVR
                     LRRDSVEFDYPAKSGVQRTIELEDPLVVRAVRSLMRAQTRLPRLLVYRSDEGDWVEMR
                     ADDVNGRFRELVGDEFSIKDLRTWHGTVLAAEAFAHSPEPTSKTARRRTESTVMKAVA
                     AELGNTPAVARASYVDPRVVQAYQDGLTIQPAILRAQRHRSPAARQELIEKATCRLIH
                     RVEKGR"
     gene            3855..>4060
                     /locus_tag="VF874_21935"
     CDS             3855..>4060
                     /locus_tag="VF874_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178625.1"
                     /translation="MDETEAVNHPVRAGSLHLRHDVHEHHGIDPTTNSERTSTTTATT
                     DATLRTACDAAGTVGDWSVRHGSG"
BASE COUNT          723 a         1451 c         1261 g          625 t
ORIGIN      
        1 gttgacccga cccgctagct gcgtatcgga tcgtccttcc cggacgactc gcggcgcggg
       61 ccgcgctagc cacgaggccg agtattacgc tcaactccag gtcggctgac gtaccgatca
      121 cacgtaacga ggtggccgcg ccacagcgtc acagcgacaa cacccggcag tgatgagttg
      181 ctccggcggg cactttgact ctcggtcggg catccgacac agccggcgca gcggcgccag
      241 cccgtggcag gaccccggcc gataagcccg gaggactcac ggggcacact ggaaacacca
      301 ccaccacgcc ggcgcgagcc ggccgggcac acctgcggag ttcggtgttc gagaacctca
      361 cctggacgca tctggcgatc atcgccgccg tcgggctgct cgtcttcggg cccgaccgcc
      421 tccccggggc gatccgccgc agcgccgcgg cgctgcggca gctgcgcgca cagctcgccg
      481 agccaccagc gggctgctcg tcgagttcgg acccgagctc gacgagctgc gcgagccgtt
      541 ggccgagctg caacggctac gcggcatgac cccgctcgcg gccatcacac gccacctcct
      601 caacggcgac gacacgggtg cggcaacgtt tgaccactcc cccgccgcac cggccgttga
      661 accgcatcca cgatgacgcc gggccaccgc ccccgccaat caccggcgaa cgataggcaa
      721 acccccgatc gaaggaaaat gaccatggcg cagaaggcag tggccaagag caccacccgc
      781 acgacggccg agcggatccg cgacctggac aaggtcgagg tcaccgtccc ggtgctcggg
      841 cagatccgca tcccgcggcc tgagcggctg gcgttctacg gcggactggc cgcgctggcg
      901 gcgctgcagc tgatcgactg gccgatcgcc gccgcgctgg cggtcggcca cctgctgtcc
      961 gaggaccatc acagccgcgt cgcgcaggaa ctcggcgctg ctcttgagga ggcctgaacc
     1021 ggccgcaaac cgcgtacccg acgccgactg cagccgcctg ccggtaactc tggcgcaggt
     1081 cgttgatgat gcgcggatgc gcgagatggc gttgtcgact tgaggtctat attgcacgat
     1141 tagtcaccgg cgctgggaaa cgctgagaat cgctggcaag actcaaagtt gctgtgccac
     1201 agcagcttct gccatctcgc ggaacaggtt catcagcgga attaacgatc gccgctccgg
     1261 ctgtcccatt tgttagacgc ctatgtcgcg ctggtgcacg acgcaggtca actcgactcg
     1321 atcagccccg gagataacgc tggtctcata caccggtcgc ctccgcccgc tacccatcgg
     1381 ccgcccgccg ccgatcccaa cgcatcgaca ccgcccgaac atgacgttcc gacttcgccc
     1441 gcccatcacc gggatggacc cgagctacca actcgaccac cgagcgctga gtcgcacgca
     1501 actcggtcgc gattttgact cgcgtcgagg ccgtggtctc ctcgcggaac atctccttga
     1561 gcacctcggc ccggtcagcc atatcagccg ccatcgcgat cacctgcagc tcggcctcgg
     1621 tccactcgac cgcgtagccg agctgcctcg acgcatgctc caacgcctta tccaagtcgg
     1681 cccgcaaccg gcgcccaggg gtcattcggg gtggcataac ccctgatcct aggccgtgaa
     1741 ggcgccgaac agggggccga cagcctcgga tcaggccccg gtgcaacacc cacggcgaca
     1801 accagacgca acgttgccag accacctcag cgacaacaac attcacgcac atcgcaaaca
     1861 ccgaacagcg ttgcggcagc tagcctttcg gtttcaccac ccaggacttg acgcgctcgc
     1921 agtgacttac gaccgaattt gatgtttggt cacaagtggg cctcgggaac cacttgtggc
     1981 acgtgcacgg ttcgtggacc gaatcgctgg gctgggccgc gatcggccga aagccctcgg
     2041 cgccgtcgtc gtcgacaccc gaaacctgct cggcgccgat gcgattcacg actgcgatct
     2101 aaactacttg ggcaacggca tagcgagcgg ctgagcgcgc cgacggccga ggacgaggcc
     2161 cagcagcagc atgccgacac ccagccccag gtgcagccag ttgtcggcgg agttcaccgg
     2221 cacgaagttg gcggcgccgt catcggccac gatgagtccg tagacccaca gcaccacgta
     2281 gatcgtcccg ctgccgatga gaaaccaccg cgcgccgtag aaggttcgcg cgagcgccaa
     2341 cccggcgaca ccgaacgcca gatgcacgat gctgtgcagc accgaaatgt cgaacaggcc
     2401 cagcagcttc gcttcggagt gatggccggc gaacgtcatc atgtcgtagt tcgtggtgat
     2461 gccgggaatg aagccgagca ctcccacgag cagaaacacc gcccccacga cgaatgccgc
     2521 taactgcacc ggcgcccggc gcgcggtcgt ctgccttgat ttactgacca tttgtcatgc
     2581 ctccttgtcg gtcttgcgtt tgggcattcg tcaccgagcc caacgaggac cgagccgcgg
     2641 gccggacgtc agaaagtcgt cacaagtgga cgtcatttcg ccgagactgt cgccagcgcg
     2701 gaaaaatgcg agaggccacc acgtggaatg caatttcggc gaacgccaga gctagcggcc
     2761 tttctcgacg cggtggatca gccggcaggt cgccttctcg atcagctcct gtcgcgcggc
     2821 cggcgagcgg tggcgctggg cccgcaggat cgccggctgg atggtcaggc cgtcctgata
     2881 ggcctgcacc acccgcgggt cgacgtatga cgctcgggcc accgccggtg tgttgccgag
     2941 ttccgctgcg accgccttca tcacggtcga ctcggtgcgg cgtcgcgcgg tcttgctcgt
     3001 cggctcgggg gagtgcgcga acgcctccgc ggcgagcacg gtgccgtgcc aggtgcgcaa
     3061 gtccttgatg ctgaactcgt cgccgaccag ctcacggaac cgtccgttca cgtcgtcagc
     3121 gcgcatctcc acccagtcgc cctcgtcgct gcggtacacc agcagccgcg gcagccgagt
     3181 ctgcgcgcgc atcagcgacc gcaccgcgcg gacgaccagc ggatcctcca gttcaatcgt
     3241 gcgctgcacg ccgctcttag ccggatagtc gaactcgacg ctgtcgcggc gcaggcggac
     3301 atgttcccgg cgcagcgtgg ccagcccgaa actgccgttc tcctgcgcgt actcttcacc
     3361 gcccgcccgg aagtagccgc ggtccagcag ccgttgcgcc acggcgagca cgcgggcgca
     3421 ctgcaacccg cgcccgcgca ggtcgctgac cacctgcgtg cgccagtccg gcaaccgctt
     3481 ggccaggctc agcgtccggt cgtacttctc ctccgcgcgc tcctgctgcc accgctggtg
     3541 gtacaggtac tgcttgcggc cggcggcgtc ggtgccgacg gcctggatgt ggccgttcgg
     3601 atacgggcag atccagacgt tcttccacgc cggcgggatg accagctctt cgatgcgggc
     3661 caacgtgtcg ggatcggtca gcggcgtgcc gccggcctcg gcgtacgaga agccgcgacc
     3721 ccggcggaca cgccgcaccc cggggccgcc gacattgctt cggcgcagcc tcacggactt
     3781 gggttttcca ctgagattta cgcctcaaac atcgccgttt aaccgcccgt ttgggccggg
     3841 tatccagagc cctgatggac gagaccgaag cggtgaatca tccggtgcgc gccgggtcat
     3901 tgcatttgcg gcatgatgtc cacgaacacc acggcataga ccccacaacc aacagcgaaa
     3961 gaaccagcac gaccaccgcc accaccgacg cgacgctgcg gaccgcttgc gatgcggccg
     4021 gcactgtggg tgactggtcg gtccggcacg gtagcggaat
//
LOCUS       DASWBF010000588         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_578939,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000588 DASWBF010000000
VERSION     DASWBF010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578939"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..807)
                     /locus_tag="VF874_21940"
     CDS             complement(145..807)
                     /locus_tag="VF874_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9178626.1"
                     /translation="MLKVVHDVDVSNENGAAGGSLLDEIVRDGARAMLAAALLAEVAA
                     YIDAHADAVDENGCRLVVRNGYHGEREVLTAAGAVPVRAPRVNDKRIDETTGERTRFS
                     SAILPAWARKSPQVAEVLPLLYLHGLSSSDFGPALEQFLGSAAGLSAATITRLTAQWQ
                     DDAEAFTKRSLTDTDYVYLWVDGIVGGSRGLRAVRRRVAPFVKGTMSRNRMEVWDQIT
                     SL"
     gene            complement(999..1193)
                     /locus_tag="VF874_21945"
     CDS             complement(999..1193)
                     /locus_tag="VF874_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178627.1"
                     /translation="MSDTTDDALFDDLSPTAIVCFRLSDSEITVWPDAATARLALDRV
                     GDTEVWPPLCRAPCPGAHRS"
     gene            1473..>1851
                     /locus_tag="VF874_21950"
     CDS             1473..>1851
                     /locus_tag="VF874_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178628.1"
                     /translation="MTTATKTLTINDLTPAERKLLGLCIHESAHATMAVALGGTLRTA
                     KVYGNRVTPGSLTDIGSSGLTLVDVVPEGRSPRRHSLGRGRRLVGWQAGGRRCGRCTQ
                     LSTRPGVATGRCWPVTGWPPVPVS"
BASE COUNT          324 a          641 c          587 g          299 t
ORIGIN      
        1 taccaaccgc gtaatgcggt tcatacaccc gatcgacacg tttcgaccag ggtcttcatc
       61 ccgtccgaac cgaagccccc cagccacacc caccggcatg cccgtcggga tggggcgaag
      121 cccggctagt ggtagccaac atagttacag tgacgtaatt tgatcccaca cttccatacg
      181 gttacgtgac atggttccct tgacgaaagg agcgacacgg cggcgaaccg ctcggagtcc
      241 ccggctgcct ccgacgatcc cgtcgaccca cagatacaca tagtcggtgt cggtcaacga
      301 gcgcttggtg aacgcctcgg cgtcatcctg ccactgggcg gtcagccggg tgatcgtcgc
      361 cgcggacagg cccgcggccg accccaggaa ctgctccaac gccgggccga aatcgctgct
      421 ggacaagcca tgcaagtaca acagcggcag cacctcggca acctgcggag acttgcgcgc
      481 ccacgccggc aagatcgccg aggagaaccg cgtgcgctcc ccggtggtct catcgatgcg
      541 cttgtcgttg acccgcggcg cccgcaccgg caccgcaccc gcggcggtca acacctcgcg
      601 ttcgccgtgg tagccgttgc ggaccaccag ccgacacccg ttctcgtcga ccgcgtcggc
      661 atgggcatcg atgtaggcgg cgacctcggc cagcaacgcc gccgcgagca tcgcccgcgc
      721 gccgtcacga acaatctcgt cgagcaacga cccgcccgcc gcgccgttct cgttggacac
      781 atctacgtcg tggactacct tgagcatggg cgtaccttcc caaccagcgc gtcaacgccg
      841 gtcttgatca gaactacttg gactttcaga tcatcctcgg gaaggtgcgt ccctacacgt
      901 cacctcgccg agggccatcc acaggttctg atcattcctc acatggaagg catcgtcgtc
      961 gacctaggcc agcgggctgg ccggtcgcag cagcccaatc atgaccgatg tgcgccaggg
     1021 catggtgccc ggcacagcgg cggccacact tcggtgtcgc caactcggtc cagtgccagc
     1081 cgcgcggtgg ccgcatccgg ccacaccgtg atctcgctgt ccgataagcg aaagcacacg
     1141 atcgcggtcg ggctcaagtc gtcgaacagg gcgtcgtcgg tggtgtcgct catcgctcgt
     1201 acccgatgac ttcgccgcga tcgttgagtt gcggcacgtc gtgcccgtct tcgcgccatg
     1261 agtcctcgcc tgcttcggcc cacgctcggc acttgcgtac gcattcgtgg cagcggcggc
     1321 gggtgattgc ctcgggcctg agcagttgtc cgcacatgtc gcagtgcgga tcgcactcat
     1381 tgcagcggag gcgcgcccag ctggtcgccg ctggccgcgg gccgggtgac cggtgggccg
     1441 cccgacccga cgacgacgaa aggacaaccg aaatgacaac agcgacaaag actctcacaa
     1501 tcaacgacct caccccggcc gaacgcaagc tgctcgggct gtgcattcac gaatcggctc
     1561 acgccacgat ggccgtggcg ctgggcggca cgctgcgcac ggcgaaggtg tacggcaacc
     1621 gcgtcacgcc ggggtcgctg actgacatcg ggtccagcgg attaaccctg gtcgacgtag
     1681 tgccggaagg gcggagcccg aggcggcatt cgctgggccg tgggcgcagg ctcgttggtt
     1741 ggcaggcagg cggccgacgc tgcgggaggt gcacgcagct ttcgacacga ccgggtgtcg
     1801 cgaccgggcg atgctggccg gtgacgggat ggccgccggt tccggtgtcg t
//
LOCUS       DASWBF010000589         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1113037,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000589 DASWBF010000000
VERSION     DASWBF010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113037"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VF874_21955"
     CDS             complement(<1..394)
                     /locus_tag="VF874_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX9178629.1"
                     /translation="MRESSNPVFRSLPKQQQGGYAQFGPGYAAGAQQVYQGYQTEPFI
                     EQKGVSRPLTIDDVVTKTGITLGVLSLVAVVSYFLVGTNPGLAMPFTMLGAFGGLALV
                     LIATFGRKQDSPAIVLSYAALEGLFLGAI"
     gene            655..1674
                     /locus_tag="VF874_21960"
     CDS             655..1674
                     /locus_tag="VF874_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9178630.1"
                     /translation="MLTHASGGVRFLILNRPKAINSLTHSMVTTMQSVLRDWAHDDDV
                     RAVVVRGAGERGLCAGGDIVAIYHSVHTDGGAEARQFWHDEYVLNHYIARYPKPCVAL
                     MDGIVMGGGVGIGVHAGTRVVTDTSKVAMPEVGIGFVPDVGGTYYLSRAPGNLGLHAA
                     LTGAPFSGSDAIAMGFADHYVPHQALDDFAQAIVDDALEEAVKSHSVAPPSSNLFAQR
                     SWIDDCYAGDTVADIVAALRGHDAGPANDAANLIATRSPTSLAVALAAVRRARRLDTL
                     EDVLRQEYRVSCATLRSHDLVEGIRAQVIDKDRDPKWSPSSLSAVSAADVEAFFAPAE
                     NDLSF"
     gene            1688..2461
                     /locus_tag="VF874_21965"
     CDS             1688..2461
                     /locus_tag="VF874_21965"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9178631.1"
                     /translation="MTYETILVDRQDRVATITLNRPQALNALNGQVMHEVTTAAAELD
                     HDSGVGAIIVTGSTKAFAAGADIKEMAELSFADAFAADYFAAWGKFAAVRTPTIAAVS
                     GYALGGGCELAMMCDVLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLIL
                     TGRNMDAAEAERSGLVSRVVPADDLQREAMSVATTIAQMSLSAARMAKEAVNRAFETP
                     LSEGLLYERRLFHSAFATDDQSEGMAAFVEKRTPNFTHR"
     gene            2472..>3339
                     /locus_tag="VF874_21970"
     CDS             2472..>3339
                     /locus_tag="VF874_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9178632.1"
                     /translation="MTDTQAASPADEVPQEEADETPEAAEPAEEPAEQPHWWQRRYTF
                     TGTAVGLVFLWLSMTPSLLPRGPLFQGLVSGGAGATGYAVGVFAVWLVRFMMSKDTSP
                     KPPPIAWLILVGVGIAGTIGMIIWFHLWQTHIRDLMGVPRLKWHDYPLAAILAIVTLF
                     VFVEIGQLVGRFIRFLVRQLNRVAPPRVSAVVAVVLVVALFITVLNGVILRFAMDTLN
                     NTFEAVNNEMDPDSPPPNTTLRSGGPGSLVTWETLGNQGRIFIDGAATVEELSKFNGA
                     PAVEPIRAYAGLE"
BASE COUNT          562 a         1125 c         1101 g          551 t
ORIGIN      
        1 agatcgcgcc gaggaacagg ccctccagcg cggcgtagct cagcacgatg gcagggctgt
       61 cctgcttgcg gccgaatgtc gcaatcagca cgagcgccag cccgccaaat gcgccgagca
      121 tggtgaacgg catcgccaac cccgggttgg tgcccaccag gaagtaggaa acgaccgcca
      181 cgagcgacag cacacccagc gtgatgccgg tcttggtgac gacgtcgtcg atggtcagcg
      241 ggcgcgacac gcccttttgc tcgataaagg gctcggtctg atagccctgg taaacctgtt
      301 gcgcaccggc ggcgtagccg gggccgaact gcgcgtatcc gccttgctgc tgcttgggca
      361 gcgaccgaaa taccgggttg cttgactccc gcaccgtcgg ttcctccctg tggtgttgcc
      421 ggaaacaacc gtctaacgat cgacggatcg tgtgggttcc cggttgtcgt ctttcgaggt
      481 aaaaccatac ccgcggcggg tagagctgcg agagacgatc tagattgctc aaccgtgacg
      541 gatgaatccc tgctcgatgg tcgctcggac gagcctcgct ccgtatctga tggtcgctcg
      601 gacgagcctc gctccgtatc tgatggtcgc tcggacgagc ctcgctccga cgagatcctg
      661 acccacgcct ccggcggcgt ccgttttctg atcctgaacc ggcccaaagc catcaactcg
      721 ctgacgcatt cgatggtcac caccatgcag tcggtcctgc gcgactgggc gcacgacgac
      781 gacgtgcgcg cggtggtagt ccgcggtgcg ggcgaacgcg ggttgtgcgc gggcggcgac
      841 atcgtcgcga tctaccacag cgtgcacacc gacggcggcg ccgaggccag gcagttctgg
      901 cacgacgagt acgtcctcaa ccactacatc gcccgctacc ccaagccctg cgtcgcgctg
      961 atggacggca tcgtgatggg cggcggcgtc ggcatcggcg tacacgccgg cacccgcgtc
     1021 gtgacggaca cctcgaaggt ggcgatgccc gaggtcggta tcggctttgt gcccgacgtc
     1081 gggggcacct actacctgtc tcgcgcgccc ggcaacctgg gtctgcacgc cgcgctcacc
     1141 ggtgcgccgt tctccggttc cgacgcgatc gcaatgggtt tcgccgacca ctacgtgccg
     1201 caccaggccc tcgacgactt cgcgcaggcg atcgtcgacg acgccctcga ggaggccgtg
     1261 aaaagccatt ccgtcgcgcc gccgtcgagc aaccttttcg cacaacggag ttggatcgac
     1321 gactgctacg ccggcgacac cgtcgcggac atcgtcgcgg cgctgcgcgg gcacgacgcc
     1381 ggaccggcca acgacgcggc caacctgatc gccacccgct ctcccacgtc gctcgccgtc
     1441 gcgctggcgg cggtgcgccg ggcgcgccgg ctcgacacac tggaagatgt tctgcgccaa
     1501 gagtatcggg tatcgtgcgc gacgttgcgc tcccacgatc tggtggaggg catccgcgcg
     1561 caagtcatcg acaaggaccg cgaccccaag tggtcgccct cctcactgtc agcggtcagc
     1621 gcagccgacg tcgaggcgtt cttcgcgccc gccgagaatg atctgtcgtt ctagaaggga
     1681 cctttcgatg acctacgaga cgatcctcgt cgaccgccaa gaccgcgtcg cgacgatcac
     1741 gctgaaccgc ccgcaagcgc tgaacgcact caacggccag gtgatgcacg aagtcaccac
     1801 ggccgcagcc gaactcgacc acgattccgg cgtcggcgcg atcatcgtca ccggctcaac
     1861 caaggcgttc gcggccggcg ccgacatcaa ggagatggct gagctgtcgt tcgccgacgc
     1921 gttcgccgcc gactacttcg cggcttgggg caagttcgcc gcggtgcgca cgccgacgat
     1981 cgcggccgtg tccgggtacg cgctcggcgg tggttgcgag ttggcgatga tgtgcgacgt
     2041 gctgatcgcc gccgacaccg caaaattcgg ccagcccgag atcaaactcg gcgtgctgcc
     2101 cggaatgggc ggctcgcagc ggctcacccg agcgatcggg aaggccaaag cgatggacct
     2161 gatcctgacc ggacgcaaca tggacgcggc cgaggccgaa cgcagcggcc tggtgtcgcg
     2221 tgtggtgccc gccgacgacc tgcagcgcga ggcgatgtcc gtcgccacca ccatcgcgca
     2281 gatgtcgctg tcggccgccc gcatggccaa ggaggcggtc aaccgcgcgt tcgaaacgcc
     2341 gctctccgag ggcctgctgt acgagcgccg gctgttccac tccgcgttcg cgaccgacga
     2401 ccagagcgag ggcatggccg cgtttgtcga gaaacgcaca ccgaacttca cacaccgctg
     2461 aagatggggg catgaccgac acacaggccg cgagcccggc cgacgaagtt ccgcaagaag
     2521 aggccgacga gacgccggaa gcggcagaac cggccgagga gcccgccgaa cagccgcact
     2581 ggtggcagcg ccgatacacc ttcaccggga cggcggtcgg gctggtgttc ctgtggttgt
     2641 cgatgacgcc gtcgctgctg ccgcgcgggc cgctgttcca gggcctggtg agcggcgggg
     2701 ccggcgcgac cggttacgcg gtcggcgtgt tcgccgtctg gttggtgcgc ttcatgatgt
     2761 ccaaggacac cagccccaag ccgccgccaa tcgcgtggct gatcctggtg ggcgtcggca
     2821 tcgccggcac catcggcatg atcatctggt tccacctctg gcagacccac atccgcgact
     2881 tgatgggtgt gccgcgactg aaatggcatg actacccgct cgctgcgatc ctggcgatcg
     2941 tcacgctgtt cgtgttcgtc gagatcggcc agctggtcgg gcggttcatc cgattcctgg
     3001 tccggcaatt gaatcgggtt gcgccgccgc gggtttcggc cgtcgtcgcg gtggttctcg
     3061 tggtcgcgct gtttatcacg gttctcaacg gtgtgatcct gcggttcgca atggacacgc
     3121 tgaacaacac cttcgaggcc gtcaacaacg agatggaccc ggacagcccg ccgccgaaca
     3181 cgacgctgcg ctcgggtggc cccggttcgt tggtgacatg ggagacgctg ggcaaccagg
     3241 gccggatttt catagacggc gccgcgaccg tcgaggagct gtcaaagttc aacggcgcgc
     3301 ccgcggtcga gccgatccgc gcatacgcgg ggttggagt
//
LOCUS       DASWBF010000590         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_128555,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000590 DASWBF010000000
VERSION     DASWBF010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128555"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VF874_21975"
     CDS             complement(<1..362)
                     /locus_tag="VF874_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178633.1"
                     /translation="MGSGRAAVGTGSAAVGNGTVAVDATVAVGSGPAAVGTGAIAVGD
                     GAAGVGNGAFAVANGAVAVGSGVVALGNCTVAVAVGSGPVAVGNGPVGVGNGPVGVGN
                     GPVGVGNGVDTGVVSADG"
     gene            complement(450..854)
                     /locus_tag="VF874_21980"
     CDS             complement(450..854)
                     /locus_tag="VF874_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 domain-containing protein"
                     /protein_id="HEX9178634.1"
                     /translation="MRDHLPPGLPPDPFADDPLDPSAALDAIEPGQPLDPQERTAVEA
                     DLADLAVYEALLAPKGIRGLVVSCDECQQDHYHDWDMLRANLLQLLVDGTVRPHEPAY
                     DPEPDAYVTWDYCRGYADASLNEATSDPDGYR"
     gene            1015..>2242
                     /gene="guaB"
                     /locus_tag="VF874_21985"
     CDS             1015..>2242
                     /gene="guaB"
                     /locus_tag="VF874_21985"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9178635.1"
                     /translation="MNSAASVPAVTPVPTGGDDPHKIAMLGLTFDDVLLLPAASDVVP
                     AAADTSSRLTKKIRLKVPLVSSAMDTVTEARMAIAMARAGGMGVLHRNLSVAEQAGQV
                     EMVKRSEAGMVTDPVTCSPDNTLAEVDAMCARFRISGLPVVDALGSLVGIITNRDMRF
                     EVDQNKRVSEVMTKAPLITAREGVTADAALGLLRRNKIEKLPIVDGHGRLTGLITVKD
                     FVKTEQHPLATKDGDGRLLVGAAVGTGDDAWVRAMTLVDAGVDVLVVDTAHAHNRLVL
                     EMVGKLKAEAGDRVEVIGGNVATRSGAAALVDAGADAVKVGVGPGSICTTRVVAGVGA
                     PQITAILEAVAACSPACVPVVADGGLQYSGDIAKALVAGADSVMLGSLLAGCEESPGE
                     LVFINGKQYKSYRGMGS"
BASE COUNT          357 a          782 c          779 g          324 t
ORIGIN      
        1 tcaccgtctg ccgagacgac gccggtgtcg acgccgttgc cgacgccgac gggtccgttg
       61 ccgacgccga cgggtccgtt gccgacgccg acgggtccgt tgcccacggc gaccggtccg
      121 ctgccgacgg cgacggcgac cgtgcaatta cccagagcca ccacgccact gcctaccgca
      181 acggctccgt tggcaacagc gaacgcgccg ttgccaactc cggcggcccc gtcgccgacc
      241 gctattgcgc cggtgccgac cgcggctgga ccgctaccca ccgcgaccgt cgcgtcgaca
      301 gccaccgtgc cgttgccgac cgcggcggag ccagtgccga cagcagcccg gccgcttccc
      361 accgcaaccg aggcgcccgc ggcgccgagg acgtcaatcg tgacgacgac ggtggcgccc
      421 gcgacgccgg aaggctagag gcggacggtt cagcggtatc cgtcgggatc cgacgtggcc
      481 tcgttcagcg acgcgtcggc atagccgcgg caatagtccc aggtcacata cgcgtcgggt
      541 tcgggatcgt aggccggctc gtgcggccgg acggtgccgt cgaccaatag ctgcagaagg
      601 ttcgcgcgca gcatgtccca gtcatggtag tgatcctgct ggcactcgtc gcacgacacc
      661 acgagcccgc gaattccctt gggcgccaac aacgcttcgt acacggcgag gtccgccagg
      721 tcggcctcga ccgcggtccg ctcttgcggg tcaagcggct gacccggctc gatcgcatcc
      781 agtgccgccg acgggtccaa cggatcgtcg gcgaacggat cgggcggcaa acccgggggg
      841 aggtggtcgc gcacgcctct acagtaacta gcccagccga catttcgcca gccgcgccca
      901 gcccattggc acggcctcgc caacgacggc gataccgtcg ataccgataa gatgagcacc
      961 tagccccgca ccgatggagg cctcccgatg tcgataacag acggtcgtac ttcggtgaat
     1021 tcggctgcct ccgtccccgc cgtcaccccg gtccccaccg gcggcgacga cccacacaag
     1081 atcgcgatgc tcgggctcac cttcgacgac gtactgctgc tgccggccgc ctccgacgtg
     1141 gtcccggccg ccgccgacac ctccagtcgg ttgaccaaga agatccgtct gaaggtgccg
     1201 ctggtcagct ccgcgatgga caccgtgacg gaggcgcgga tggcgatcgc gatggcccgc
     1261 gcgggcggca tgggagtgct gcaccgcaac ctgtcggtcg ccgaacaggc cggccaggtc
     1321 gagatggtca agcggtcgga ggccggcatg gtcaccgacc ccgtcacgtg ctcgccggac
     1381 aacacgctgg ccgaggtcga cgcgatgtgc gcgcggttcc ggatctccgg cttgccggtg
     1441 gtggacgcgc tcggctcgtt agttgggatc atcaccaacc gggacatgcg cttcgaggtg
     1501 gaccagaaca agcgggtgtc ggaggtcatg accaaggctc cgctgatcac tgcccgagag
     1561 ggcgtcaccg ctgatgcggc cctgggattg ttgcggcgca acaagattga gaagttgccg
     1621 attgtcgacg gacacggacg gctcaccggg ctgatcacgg tcaaggactt cgtcaagaca
     1681 gaacagcatc cgctggccac caaggacggc gacggccggt tgctggtcgg cgccgccgtc
     1741 ggcaccggcg acgacgcctg ggtgcgcgcg atgacgctcg tggacgccgg tgtcgacgtg
     1801 ctcgtggtgg acaccgcgca cgcgcacaac cggctggtgc tcgagatggt cggcaagctc
     1861 aaggccgagg ccggcgaccg cgtcgaggtc atcggcggca atgtcgccac ccgcagcggg
     1921 gctgcggcgc tggtggacgc cggcgcggac gcggtgaagg tgggcgtcgg gcccggctcg
     1981 atctgcacca cccgggtggt ggccggcgtc ggggcgccgc agatcaccgc gatcctggag
     2041 gcggtcgcgg cctgctcgcc ggcctgtgtg ccggtggtgg ccgacggcgg actgcagtac
     2101 tcgggcgaca tcgccaaggc gctggtggcc ggcgcggact cggtcatgct cggctccttg
     2161 ctggccggct gcgaagagag ccccggcgag ctcgtcttca tcaacggcaa gcagtacaag
     2221 tcgtatcgcg gcatgggctc cc
//
LOCUS       DASWBF010000591         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_129195,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000591 DASWBF010000000
VERSION     DASWBF010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129195"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..2016)
                     /locus_tag="VF874_21990"
     CDS             complement(343..2016)
                     /locus_tag="VF874_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX9178636.1"
                     /translation="MGKRRGNQTYYYLVESARVDGKPRIVSQQYLGSADEVLAKLSET
                     PAGGPKRSRHKRFGDLAAVWSTLVRLDVAGIVDEVAPRRADAAASVGTYLALACANRI
                     VDPCSKRGFADWWATTAGPRWVKLGRAAVDHRRFWDAMDRLGEAELRAIETRLGRAMV
                     TEFGLDLTGLALDMTNFATFIDTGNQRAPIAQRGKAKQKRTDLRLVGLALVVTRDGGV
                     PVISHAYPGDRPDVTQFTTVIDELVTRYRDLVSGVESLTVVYDAGQNSGDNHAVVEAH
                     GIGFVGSLPPSDHPDLLAIADHDYRPVDTGRYPGLRYLDTTVTALGVTRRAVLTHSAN
                     LHAKQSRGLDQTLATARRRLTDLAARLARGRTRRDRAAVEAEIAAIRRPRWVADIITV
                     TLTGQAPAELRLTWRTDARARTRLEARLFGKRILFTNRDWPVPDVVAAYRSQSDAEFG
                     FRQLKDPHVVSFSPMHHWTDSKIRVHVFYCVLALAVAHLMRRHAAHAGLHMSVRELLD
                     ELAGIEETELLYHDGGKGRPRVQRMLTDMNPTQQQLADLFDIHHYAPTR"
     gene            complement(2234..2605)
                     /locus_tag="VF874_21995"
     CDS             complement(2234..2605)
                     /locus_tag="VF874_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178637.1"
                     /translation="MEPQSLEARVAALETQVHELAERVRASEQDAAAARVLAGAADRD
                     VAEFGDEIRDFRQATVGSFNALREDFGDLRTKVERGFTEMRGKLDAGAAGQQRIVELI
                     EGLIADRGENGPPGPEQSRSQ"
BASE COUNT          446 a          960 c         1023 g          534 t
ORIGIN      
        1 cgtatgaggc ttatgcggtt agcagaacgt ccacacgacc tcaagttgac agtgccgtcg
       61 cgcaagtcct cgacctctcc ggcctgcgcc cgctgcaagg cggtgacctt ctcggcgagc
      121 tggagaggtc agcggtgtca gccaatcacg accgagtcct agtcgcgacc ggtcaaatat
      181 ccggttctgg aacgacttcc gtgccgtgat ctgcctcggc ggcagtgatg gcatggcctc
      241 tagcgggagt ttccgggctt gagtgatcgt tggtctggtt tcgcctggta ggcgggtgtt
      301 ttcgggtggg gcgggtgtat tacccaactc gttgtggggg gttcagcggg tgggcgcgta
      361 gtggtggatg tcgaacaggt cggccagttg ctgctgggtg gggttcatgt cggtgagcat
      421 gcgttgcacg cgggggcgac ctttgccgcc gtcgtggtag agcagttcgg tctcttcgat
      481 gccggcgagt tcgtcgagca gttcgcgcac cgacatgtgc aggccggcgt gggcggcgtg
      541 gcggcgcatc aggtgggcga cggccagggc cagcacgcag tagaacacgt gcacccggat
      601 cttggagtcg gtccagtggt gcatcgggct gaatgagacc acgtgcgggt ctttgagttg
      661 gcggaacccg aactcggcgt cggattggga gcggtaggcg gccaccacgt cgggcaccgg
      721 ccagtcgcgg ttggtgaaca ggatccgttt gccgaacagg cgcgcctcga gccgggtccg
      781 ggcgcgggcg tcggtgcgcc aggtcagtcg cagctcggcg ggtgcctggc cggtcagcgt
      841 gacggtgatg atgtcggcga cccagcgggg tctgcggatc gcggcgatct cggcctccac
      901 tgcggcgcgg tcgcggcggg tgcggccgcg ggccagccgg gcggccaggt cggtgagccg
      961 gcgccgcgcc gtggccaggg tctggtccag gccgcgggat tgcttggcgt gcaggttcgc
     1021 cgaatgggtc agcaccgcgc ggcgggtcac gcccaacgcg gtgaccgtgg tgtcgaggta
     1081 gcgcagcccg gggtagcggc cggtgtccac cggccggtag tcgtggtcgg cgatcgccag
     1141 caggtcgggg tgatcgctgg gcggcagcga gccgacgaag ccgatcccgt gggcctcgac
     1201 gacggcgtgg ttgtcgccgc tgttctgccc ggcgtcatag accacggtca gcgactcgac
     1261 ccccgacacc aggtcccggt accgggtcac cagttcgtcg atgaccgtgg tgaactgggt
     1321 gacatcggga cggtccccgg gataggcgtg gctgatcacc ggcaccccgc cgtcgcgggt
     1381 caccaccagc gccaacccga ccagccgcag gtcggtgcgt ttctgcttgg ccttgccgcg
     1441 ctgcgcgatc ggcgcgcgct ggttgccggt gtcgatgaag gtggcgaagt tggtcatgtc
     1501 cagcgccagc ccggtcaggt cgagcccgaa ctcggtgacc atcgcgcggc cgagccgggt
     1561 ctcgatcgcc cgcagctcgg cctcgccgag ccggtccatc gcgtcccaga accggcggtg
     1621 atccaccgcg gcccgaccca gtttcaccca tcgcggcccg gccgtggtgg cccaccagtc
     1681 ggcgaaacca cgcttggagc acgggtccac gatccggttc gcgcacgcca acgccaggta
     1741 ggtgcccacc gacgcggccg cgtcggcgcg gcgcggcgcc acctcatcca cgatcccggc
     1801 cacatccagc cggaccagcg tcgaccacac cgccgcgaga tctccgaacc gcttgtgccg
     1861 gctgcgtttc ggtcctcccg ctggcgtctc ggacagcttg gcgagcacct cgtccgcgct
     1921 gcccagatac tgctgagaca cgatgcgcgg cttgccgtcc acgcgggccg actcgaccag
     1981 gtagtagtag gtctggttac cgcgtcgctt acccacgatc gacgccatat atgggtaata
     2041 cactcttgtc acacaaacct caagcaccac aacacaagac acgccgatca gctacaaacc
     2101 ccggaaactc cgctagcagt cgcccgatcc aggtgacgtc gtcacccgcg gccgcgaggt
     2161 ctgtccacgc gccggcgcga aatatcggcg gctatcgagc gccgccgtgc cgcgggcgcc
     2221 gttggtgagg cagttactgt gaacggcttt gttcagggcc gggtgggccg ttttcgcctc
     2281 gatcggcgat caagccctcg atgagttcaa cgattcgctg ttgtcccgcc gctccggcat
     2341 cgagcttgcc gcgcatctcg gtaaacccgc gttccacctt cgtccgaagg tcgccgaaat
     2401 cctcccgcaa agcattgaag ctgcccactg tggcctgacg gaagtcgcga atttcgtcgc
     2461 cgaattccgc cacgtcgcgg tccgcagcgc cggccagcac acgcgcggca gccgcatctt
     2521 gctcacttgc gcgtacccgc tcggcaagct cgtgcacctg ggtttccagc gcagcgacgc
     2581 gcgcctcaag gctttgcggc tccacgtacc gaagcctaac tgcccgtcgc cgacgtttct
     2641 tggggccagg tgatgcactg ctgaggctca ttcggatgtt tctggggctg gataggtccg
     2701 acttgcccgc gtcagtttga cgggcggatg tcgggtcggg ttgagtgatg tcggtctcca
     2761 acgcggccgg ttgcaacgca aaccatgcgg acggttacgc ggaggacgct tatgaacccg
     2821 gccggccatg ttgagctctc aacacaggac ctggaggaac tcgcgtggga gtttctcggt
     2881 tcggagttca ccaccgaggt tcacattgac tggcccattg accggtgcgt gcacgcctat
     2941 ctgttgcatc acggactcat cga
//
LOCUS       DASWBF010000592         5325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_326449,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000592 DASWBF010000000
VERSION     DASWBF010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326449"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..457
                     /locus_tag="VF874_22000"
     CDS             185..457
                     /locus_tag="VF874_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9178638.1"
                     /translation="MTVVMETTATDYHVGACTRDPDRWLTATDEATKSVCRACPRRWL
                     CAKEACEMPAAQGMWAGVVIPEAGRGRTFALKQLRSLAEHGGYAVR"
     gene            959..>5325
                     /gene="gltB"
                     /locus_tag="VF874_22005"
     CDS             959..>5325
                     /gene="gltB"
                     /locus_tag="VF874_22005"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX9178639.1"
                     /translation="MAPSSVGLYNPAFEHDACGVAMVVDMYGRRSRDIVDKAITALLN
                     LEHRGAQGAEPHTGDGAGILIQVPDDFLRAVVDFDLPEPGSYATGIAFLPQSSKDAMA
                     ACAAVEKIVEGEGLEVLGWRDVPTDESSLGALARDAMPTFRQVFIAGASGMELERRVY
                     VIRKRAEHELGTKGPGQDGPGRETVYFPSLSGQTFVYKGMLTTPQLKAFYLDLQDDRL
                     TSALGIVHSRFSTNTFPSWPLAHPFRRIAHNGEINTVTGNENWMRAREALIRTDVFGS
                     DVEKLFPICTPGASDTARFDEVLELLHLGGRSLAHAVLMMIPEAWERHESMDARRRAF
                     YEYHASLMEPWDGPASMTFTDGTIVGAVLDRNGLRPSRIWVTSDGLVVMASEAGVLDL
                     DPSTVVKRMRLQPGRMFLVDTTQGRIVDDEEIKAELAAEHPYQDWLDSGLIALEELPQ
                     GPYVRMPHHRIVLRQLVFGYTYEELNLLVAPMVRTGAEPLGSMGTDTPVAVLSQRARM
                     LYDYFQQLFAQVTNPPLDAIREEVVTSLQGVIGPEGDLLSPDENSCRQIVLKQPILRN
                     HELAKLVNLDPDADINGRRHGMRTKLIRCLYPVAKGGAGLRAALDDVREQASAAIADG
                     ARIIVLSDRESDEKLAPIPSLLSVAAVHHHLVRERSRTLVGLVVEAGDAREVHHMAAL
                     IGFGAAAINPYMAFESIEDMLDRKVIAGLGREKALANYVKAAGKGVLKVMSKMGISTL
                     ASYTGAQLFQAVGISQPVLDEYFTGMTCPTGGIDLDDIADDVAKRHALAFLPRPEERA
                     HRELEVGGEYQWRREGEYHLFNPDTVFKLQHATRTGQYKIFKEYTKLVDDQSERIASL
                     RGLLRFRDGARPPVPIEEVEPASEIVKRFSTGAMSYGSISAEAHETLAIAMNRLGGRS
                     NCGEGGEDVRRFERDENGDWRRSAIKQVASGRFGVTSHYLTNCTDLQIKMAQGAKPGE
                     GGQLPGSKVYPWIADVRHSTPGVGLISPPPHHDIYSIEDLKQLIHDLKNSNPQARVHV
                     KLVSENGVGTVAAGVSKAHADVVLISGHDGGTGATPLTSMKHAGAPWELGLAETQQTL
                     LLNGLRDRIVVQVDGQLKTGRDVAIATLLGAEEYGFATAPLVVSGCIMMRVCHLDTCP
                     VGVATQNPELRKRYNGKPEFVENFFMFIAEEVREYLAQLGFRTINEAVGQVGALDTTL
                     AAEHWKARKLDLSPVLHEPESAFMNQDLYCSSRQDHGLDKALDQQLIVMSREALDQQK
                     PVKFSTTISNVNRTVGTMLGHELTKAYGGQGLPDDTIDITFDGSAGNSFGAFVPKGIT
                     LRVYGDANDYVGKGLSGGRIVVRPARNAPDGYVAEDNIIAGNVILFGATSGEAFLRGQ
                     AGERFAVRNSGAHAVVEGVGDHGCEYMTGGKVVVLGPTGRNFAAGMSGGVAYVYDPTR
                     VLPDHLNSEMVELEELDDD"
BASE COUNT          937 a         1719 c         1814 g          855 t
ORIGIN      
        1 atcgagaatc actttatccg ccagatcggc gctggcagac gagcgagacc gtatcgacgc
       61 tatggtttcg cgacccggat cgggtggatt cggcttccgc aggcgcgtgc tccatcgaac
      121 aaaccgttgc gctgctacgt aataactgct acggtttgat ccattccgtc gggaggggct
      181 tgcgatgacc gtggtgatgg agacgacagc gaccgactac catgtcggcg cctgcacgcg
      241 cgatcccgat cggtggctga ccgcgaccga cgaggccact aagtcggtct gccgggcctg
      301 cccgcgccgg tggctgtgcg cgaaggaggc gtgcgagatg cccgcggcgc aaggcatgtg
      361 ggcgggtgtg gtcattcccg aagcgggccg cggacgcacg ttcgcgctga agcagctgcg
      421 gtcattggcc gagcacggcg gttacgcggt ccgctagcag gcccgacggg ggaggccttg
      481 ctgctggccg tggtcgagcg aggggtgggc cacggccgcc tgcagcaaac gatgagaatc
      541 gccttaacct gcgctttctt gttctgtagc cacctacccc gagatgtcag caaacgtcgc
      601 tgcggtttct ccgcgggccg agccgccctg gcgtaacatg aattgacaag cggcacagcc
      661 tattcgggtc aggccctgga ccgcttgggg gacgatcgct gaaggacggc gacggggcgg
      721 ttagggtgga ctaagttggc atgtcaaggg ccttttgtca tatcgttttc tccacttgtc
      781 gtgaccggga caagtgcgcc gttcagcgtc gtgcggctgc attcctttcg tgggattcgg
      841 cccctctttg cggccggagt gatccggcac agaattgaca agtccgaggg gcgcaaccca
      901 ccgcaaaggg agcacaccgt cggcaactaa cagaactttc gcgaagggct aggtgggaat
      961 ggcgcccagc agtgtcgggc tctacaaccc cgcgttcgaa cacgacgcat gtggcgtcgc
     1021 gatggtcgtc gacatgtacg gccgccgtag ccgcgacatc gtcgacaagg cgattaccgc
     1081 actgctgaac ctggagcatc gcggcgccca gggcgccgag ccacacaccg gtgacggtgc
     1141 cggaatcttg atccaggttc ccgacgactt cctgcgcgca gtcgtcgact tcgacctacc
     1201 cgagcccggc agctacgcga cgggcatcgc gtttctgccg cagtcgtcca aagacgcgat
     1261 ggccgcctgc gcggcggtgg agaagatcgt cgagggcgag ggactcgaag tgctcggttg
     1321 gcgcgacgtg cccaccgacg agtcgtcgct gggcgcgctg gcgcgcgacg cgatgccgac
     1381 cttccgccag gtgttcatcg ccggtgcgtc gggcatggag ctggagcggc gcgtctacgt
     1441 catccgcaag cgtgccgagc acgagctggg caccaagggc ccgggccagg acggccccgg
     1501 ccgcgaaacc gtctatttcc cgagcctttc cgggcagacc ttcgtctaca agggcatgct
     1561 caccacgccg cagctcaagg cgttctacct tgacctgcaa gacgaccggc tgaccagcgc
     1621 gctgggcatc gtgcattccc ggttctcgac gaacacgttc ccgtcgtggc cgctggccca
     1681 cccgttccgg cggatcgccc acaacggcga aatcaacacc gtcaccggta atgagaactg
     1741 gatgcgtgcc cgtgaggcgt tgatccgcac cgacgtcttc gggtccgacg tcgagaagct
     1801 gttcccgatc tgcaccccgg gcgcgtcgga caccgcccgc ttcgacgagg tgctcgagtt
     1861 gctgcacctc ggcggacgca gcctcgcgca cgcggtgctg atgatgatcc ccgaggcgtg
     1921 ggagcgccac gaaagcatgg acgcccgtag gcgggctttt tacgagtacc acgcgtcgct
     1981 gatggagccg tgggacggcc cggcgtcgat gacgttcacc gacggcacga tcgtgggcgc
     2041 ggtgctcgac cgcaacggcc ttcggccatc acggatctgg gtgaccagcg acgggctggt
     2101 cgtgatggcg tccgaggccg gcgtgctcga cctcgacccg tcgacggtcg tgaagcggat
     2161 gcggctgcag ccgggccgga tgttcctggt cgacaccacc cagggccgca tcgtcgacga
     2221 cgaggagatc aaggcggagc tggccgccga gcacccgtac caggactggc tggacagcgg
     2281 gctcatcgcg ctcgaggagt tgccgcaggg cccgtacgtg cggatgcccc atcaccggat
     2341 cgtgttgcgg cagttggtgt tcggctacac ctacgaggag ctgaacctgc tggtcgcgcc
     2401 gatggtgcgc accggcgccg agccgctggg ctcgatgggc accgacaccc ccgtcgcggt
     2461 cctgtcgcag cgggcgcgga tgctctacga ctacttccag cagctgttcg cccaagtcac
     2521 caacccgccg ttggacgcga tccgcgagga agtggtgacc agcctgcagg gcgtgatcgg
     2581 cccggagggc gacctgctgt cgcccgacga gaactcgtgt cgccagatcg tgctgaagca
     2641 gccgatcctg cgcaaccacg aactcgccaa actggtcaac ctcgacccgg acgccgatat
     2701 caacggccgc cgacacggca tgcgcaccaa attgattcgc tgcctgtacc cggtggccaa
     2761 gggtggcgcc gggctgaggg ccgcgctcga cgacgtgcgc gagcaggcat cggcggcgat
     2821 cgccgacggg gcccgcatca tcgtgctgtc cgaccgcgag tccgacgaga agctggcgcc
     2881 gatcccgtcg ctgctgtccg tcgccgcggt acaccaccac ctggtgcggg agcggtcacg
     2941 cacgctggtc ggcctggtgg tcgaggcggg cgacgcccgc gaggtccacc acatggccgc
     3001 gctgatcggc ttcggcgccg cggcgatcaa cccgtacatg gcgttcgagt cgatcgagga
     3061 catgctcgac cgcaaggtga tcgccgggct gggccgggag aaggcactcg ccaactacgt
     3121 caaggctgcc ggcaagggcg tgctgaaagt gatgtccaag atgggcatct cgacgcttgc
     3181 gtcctacacc ggcgcccagc tgttccaggc cgtcggcatc tcgcagccag tgctcgacga
     3241 gtacttcacg ggcatgacct gcccgaccgg cggcatcgac ctcgacgaca tcgctgacga
     3301 cgtcgcgaaa cgccacgcgc tggcgttcct gccgcggccg gaggagcggg cacatcgcga
     3361 gctcgaggtg ggcggggagt atcagtggcg ccgcgagggc gaatatcacc tgttcaaccc
     3421 cgacaccgtg ttcaagctgc aacacgcaac ccgcaccggg cagtacaaga tcttcaagga
     3481 gtacacgaag ctcgtcgacg accagtccga gcggatcgcg tcgctgcgcg ggctgctgcg
     3541 gttccgggac ggtgcacggc cgccggtgcc gatcgaagag gtcgagcccg ccagcgagat
     3601 cgtcaagcgg ttctcgacgg gcgcgatgag ctacggctcg atttcggccg aggcgcacga
     3661 aacgctggcg atcgcgatga atcgccttgg cggccggtcg aactgcggcg agggcggcga
     3721 agacgtccgg cgcttcgagc gtgacgagaa cggtgactgg cgacgtagcg cgatcaaaca
     3781 ggtcgcctcc gggcggttcg gcgttacctc gcactacctg accaactgca ccgatctgca
     3841 gatcaagatg gcgcagggcg cgaaacccgg tgagggcggc cagcttccgg gcagcaaggt
     3901 gtatccgtgg attgccgatg tccggcattc gacgcccggt gtggggttga tctcaccgcc
     3961 gccgcaccac gacatctact cgatcgagga tctcaagcag ctgatccacg acttgaagaa
     4021 ctccaacccg caggcccgtg tgcacgtgaa gctggtgtcg gagaacggtg tcggcacggt
     4081 cgcggcgggt gtgtccaagg cccatgccga cgtggtgctg atctccggcc acgacggcgg
     4141 caccggggcg acgccgctga cgtcgatgaa acacgccggc gcgccctggg agctcggact
     4201 ggcagagaca cagcagacgt tgctgctcaa cggtttacgg gatcggatcg tcgtgcaggt
     4261 cgacggccag ctcaagacgg gccgtgacgt ggcgatcgcg accctgctgg gcgccgagga
     4321 gtacggtttt gcgaccgcgc cgctggtggt gtcgggctgc atcatgatgc gggtatgtca
     4381 cctcgacacc tgcccggtcg gcgtggcgac ccagaacccc gagctgcgca agcgctacaa
     4441 cggcaagccg gagttcgtcg agaatttctt tatgttcatc gccgaagaag tgcgggagta
     4501 cctggcgcag ttgggttttc gcaccattaa cgaggcagtc ggccaggtcg gcgcgctgga
     4561 caccacgctg gccgccgaac attggaaggc ccgcaagctc gacctgtcgc cggtgctgca
     4621 cgagcccgag tcggcgttca tgaaccagga cctctactgc agctcgcgcc aagaccatgg
     4681 cctggacaag gcgctggatc agcagctgat cgtgatgagc cgtgaagcgc tggaccagca
     4741 aaagccggtc aagttctcga cgacgatctc caacgtcaac cggaccgtcg gcacgatgct
     4801 gggccacgaa ctcaccaaag cctatggcgg acaagggctt cccgacgaca ccatcgacat
     4861 cacgttcgac gggtcggccg gcaacagctt cggcgccttc gtgccgaagg gcatcacgct
     4921 gcgcgtatac ggcgacgcca acgactacgt cggcaagggg ctctccggcg ggcgcatcgt
     4981 cgtgcggccg gcgcgcaacg caccggacgg atacgtagcc gaggacaaca tcatcgccgg
     5041 caacgtcatc ctgttcggcg ccaccagcgg cgaggcgttc ctgcgcgggc aggccggtga
     5101 acggttcgcg gtgcgcaact ccggggcgca tgccgtcgtg gagggtgtcg gcgaccacgg
     5161 ctgcgagtac atgaccggcg gcaaggtcgt cgttcttgga cccaccggac gcaacttcgc
     5221 ggcgggcatg tccggcggtg tcgcgtatgt ctacgacccg acgcgcgtgc tgcccgacca
     5281 tctgaactcg gagatggtcg aactcgagga actcgacgac gacga
//
LOCUS       DASWBF010000593         6337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1182682,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000593 DASWBF010000000
VERSION     DASWBF010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182682"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VF874_22010"
     CDS             <1..1092
                     /locus_tag="VF874_22010"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9178640.1"
                     /translation="PAPFGFDAPTEIVRDMIQALPHAQGYSNSKGVLPARRAVVTRYE
                     LVEGFPRFDVDDVFLGNGASELITMTLQALLDDGDQVLVPAPNYPLWTAATSLAGGTP
                     VHYLCDESQGWQPDIADIEAKITDRTKALVVINPNNPTGAVYTRETLTQIAELARKHQ
                     LLLLSDEIYDKILYDDATHISMASVAPDLLCLTFNGLSKAYRVAGYRAGWLVITGPKD
                     HARSFLEGIGLLANMRLCPNVPAQHAVQVALGGYQSIDDLVLPGGRLREQRDVAWSKL
                     NEIPGVSCVKPQGALYAFPRLDPEVYGIVDDEKLALDLLLDEKILVTQGTGFNWPTPD
                     HLRIVTLPWASELADAIDRIGDFLTSYRQ"
     gene            1089..2309
                     /locus_tag="VF874_22015"
     CDS             1089..2309
                     /locus_tag="VF874_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011594270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX9178641.1"
                     /translation="MTAIASPPETHVAPRVIVAVLLLVIPLSQIGLDVYTPALPTMAA
                     EFAASNDVVQNTVTAYMLGMALAFIPVGLIADALGRKRVLLGGLMLLAGASVGCALAP
                     NLSLLMGMRFVQGIGAGACLLLAATIAADCFRGAKLVSVLGLLGAAWGAAPVLAPAVG
                     GIIVQFGSWRLVFELFALLAASVAVLVVKVLPETLPTDLRSRVDLRAATRVLGEALKH
                     RMFVGFVVMFGLIGAVQMVFGVVGPFLYEVKLGFSPATYGLVALLIGVANLTGELGCG
                     ALAQRLTTLRLASGAWAVFFLGAVVLVISADVVGVNAWVITVGAAVALVGIGVLDPQS
                     KGLAMGVFTRNIGLIAGLVNTCCYLIVSAAMALMAYLPENSQAPLGWLWVAAGAVFAA
                     VLITTVSPRRSTVH"
     gene            2319..3095
                     /locus_tag="VF874_22020"
     CDS             2319..3095
                     /locus_tag="VF874_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9178642.1"
                     /translation="MFRTVIVDQPQPGVAVLRLNRPERLNAINEVMQSELKQAFESLG
                     SDTTVSAVVLTGEGRGFCSGIDVRDFGPGMLEATAPAIDRLRFQESMAELPQAIWDLP
                     RPVIAAVNGPCVGAGLALCLAADIRICSTAATFGNAAILLGLSGAEMGMSYFLPRIVG
                     TSVAAEWMLTGRTVSAEEADRRGLVSQLVAPDELFARALEIASAIAQLTPLGVQLTKR
                     ALQVNTDAAGLTAALELENRNQVLSHATDEAAERRRKWSS"
     gene            3092..4225
                     /locus_tag="VF874_22025"
     CDS             3092..4225
                     /locus_tag="VF874_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9178643.1"
                     /translation="MTGPLRGVRIVMMGGLGPGPFCGMLLGDLGADVLRVDQPRQVDG
                     PLPIDYTVRRNQRSVALDVKQPRGRQLVHRLVENADAFVDVFRPGVAQRLGIGPDDLR
                     DVNRRLVYARMTGYGQDGPYADRAGHDINYIALAGALHGIGTEDTPVPPLNLVGDYGG
                     GGMLLAVGLLSAILEARESGQGQVLDVAMVDGAAALMAPYFGMVPAGTWRDHRHDNLL
                     DGAAHFYGTYETADGKHVAVGALERQFYAELCERLGVDVPRGDDDPSAWKAHRETFAA
                     TFRQKTRDEWERELGSPGVCAAPVLSIREAPHHPHNVARGSFIDVDGVMQPAPAPRFS
                     RTVPPPPTPPGLAGDHTRDVLGELGLDDGAVAELIDAGVARQR"
     gene            4269..5564
                     /locus_tag="VF874_22030"
     CDS             4269..5564
                     /locus_tag="VF874_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9178644.1"
                     /translation="MAWDFSTDPDWAAQLKWVDDFVRSECEPIDLIVKESHDLNDPVR
                     QALIPPLQKIVKDRGLWATHLGPHLGGPGYGQVKLALLNEILGRSECAPIVFGSQAPD
                     SGNSEILAHYGTPELKERYLEPLLDNRIVSCFSMTEPQGGADPKVFTTAAVQDGDHWI
                     INGEKWFSSFASMASFLIVMAMTDPDAPPYERYSMFVVPAETPGINVLRDVGLGYQPR
                     GGGREGYVRYEDVRVPADHMLGPRGGAFVVAQTRLGGGRIHHAMRTVGLVRRIFDMLC
                     ERAVSRHTQGELLANKQLVQEMVADSWMEIEAFRLLTLQTAWKIDQHNDYKAVRADIS
                     AVKAMMQKVLHDVSARALQLHGSLGTTHEMPFVQYLVESFVLGLADGPTEVHKVTLAR
                     LLLKDRKPSPDVFPSEHLLRLRAAAEAKFADKLAGIDRR"
     gene            complement(5561..6316)
                     /locus_tag="VF874_22035"
     CDS             complement(5561..6316)
                     /locus_tag="VF874_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9178645.1"
                     /translation="MVTGTSRGLGKAIAQQLAREGATVALTARTMDPDPKYHGSLRQT
                     LDEITADGGSAVAVQADLSKTDDRDRLFGEVVERVGAPDILVNNAAVTFLRPLDTFPE
                     RRVRLMVEMHVLAPLHLAQLAIPGMRERGRGWILNVTSVGGDLPEGPPFSEFDRAAGF
                     GIYGTVKAALNRLTKSLAAELYDDGIAVNAAGPSNPVATPGAGTLDLAKTDTEDIELI
                     TQTALTLCTGDPKTLTGRIAHTQPFLREIGWLA"
BASE COUNT          987 a         2093 c         2175 g         1082 t
ORIGIN      
        1 ccggcgccgt tcgggttcga cgcgcccacc gagatcgtcc gcgacatgat ccaggcgctg
       61 ccgcatgccc agggctactc caactccaag ggagtccttc cggctcgccg cgcggtcgtg
      121 acgcgctacg aactcgtcga gggcttcccg cgcttcgacg tcgacgacgt cttcctcggc
      181 aacggcgcat ccgaactgat cacgatgacg ctgcaggcgc tgctcgacga cggcgaccag
      241 gtcttggttc cggcgccgaa ctatccgcta tggacggcgg cgacgtcgct tgccggcggc
      301 accccggtgc actacctgtg cgacgagtcg cagggatggc agcccgacat tgccgacatc
      361 gaggccaaga tcaccgaccg caccaaggcg ctcgtcgtga tcaaccccaa caacccgacc
      421 ggggcggtct acacccgtga aacactcacg cagatagccg aattggcccg caagcatcag
      481 ctgctgctgc tgtccgacga gatctacgac aagatcctct acgacgacgc cacccacatc
      541 agcatggcgt cggtggcacc cgacctgttg tgcctgacgt ttaacggcct gtcgaaggcg
      601 taccgggtcg ccggctaccg ggccggctgg ctggtgatca ccggacccaa ggaccatgcc
      661 cgaagcttcc tggaaggcat cggcctgctg gccaacatgc ggctgtgccc gaatgtgcct
      721 gcgcagcatg cggttcaagt cgctctcggc ggataccaga gcatcgacga cctggtcctg
      781 cccggtgggc ggctacgcga gcagcgcgac gtggcctgga gcaagctcaa cgagattccc
      841 ggggtgtcgt gcgtgaagcc tcaaggtgcg ctgtacgcgt ttccgcgact cgaccccgag
      901 gtgtacggca tcgtcgacga cgagaagctg gcgctcgacc tactgctgga cgagaagatc
      961 ctggtcaccc agggcaccgg attcaactgg ccgacacccg atcacctgcg gatcgtgact
     1021 ctgccgtggg catccgagct ggccgacgcg atcgaccgga tcggcgattt cctgacgagc
     1081 taccggcaat gaccgcgatc gcgtcgccgc ccgaaacaca tgtggctcca cgcgtcatcg
     1141 tcgcggtgct gctgctggtc atcccgctga gccagatcgg gctggacgtg tacacgccgg
     1201 cccttcccac catggcggcc gagttcgccg cgtcgaacga cgtcgtccag aacacggtga
     1261 ccgcctacat gctcggcatg gcgctggcgt tcatcccggt ggggttgatc gcggatgcgt
     1321 tgggccgcaa gcgtgttttg ctcggcgggc tgatgctgct ggcaggcgcg agcgtcggat
     1381 gcgctctcgc gcccaatctt tcgctgctga tgggaatgcg gttcgtgcag ggcatcggcg
     1441 ccggcgcgtg cctgctgctg gccgcgacga tcgccgccga ctgtttccgc ggcgccaagc
     1501 tggtgtcggt gctggggctg ctcggggcgg cgtggggcgc ggccccggtg ctcgctccgg
     1561 ctgtcggcgg catcatcgtg cagttcgggt cgtggcgtct ggtgttcgaa ttgttcgcgc
     1621 tgctggcagc gtcggtggcc gtgctggtgg tgaaggtgct tcccgagacg cttccgacag
     1681 atctgcgctc acgtgtcgat ctgcgcgcgg cgacgcgggt gctcggtgaa gcgctgaagc
     1741 accggatgtt tgtcgggttc gtggtgatgt tcggcttgat cggagcggtg cagatggtgt
     1801 tcggcgtcgt cggcccgttc ttgtacgagg tgaaactcgg cttctcgccc gccacgtacg
     1861 gcttggtcgc gttgctgatc ggagtcgcga acctgaccgg cgaattgggc tgcggcgcgc
     1921 tggcccagcg gctgaccacg ttgcggctgg cctcaggtgc gtgggccgtg ttcttcctcg
     1981 gcgccgtcgt gctggtgatc tccgccgacg tggtgggcgt caatgcgtgg gtgatcacgg
     2041 tgggcgcggc ggtcgcgctg gtcgggatcg gtgtgctcga cccgcagagc aagggcctgg
     2101 cgatgggagt gttcacccgc aacatcgggc tgatcgccgg tctggtcaac acctgctgct
     2161 atctgatcgt cagcgcggcg atggccttga tggcctacct gccggagaac tcgcaggcgc
     2221 cgctcggatg gctctgggtt gcggccggcg cggtgttcgc cgcggtgctg atcaccacgg
     2281 tgtcgccgcg acggtcgacg gtgcattaaa gtccgaaggt gtttcggaca gtcatcgtcg
     2341 accagcccca accgggagtg gcggtgctgc ggctgaaccg ccccgagcgc ctgaacgcga
     2401 tcaacgaagt catgcagtcg gagctgaagc aggcgttcga gtcgctgggc tccgacacca
     2461 cggtttccgc cgtcgtcctg accggcgagg gtcgcgggtt ctgctcgggt atcgacgtgc
     2521 gtgacttcgg gcccggaatg ctggaggcga cggctccggc catcgaccgg ctgcggtttc
     2581 aggagtcgat ggcggaactg ccgcaggcga tctgggactt gccgcggcct gtgattgcgg
     2641 ccgtcaacgg cccgtgtgtc ggtgccggat tggcgttgtg tctggccgct gacatcagga
     2701 tctgttctac cgcagcgact ttcggcaatg ccgcgatcct gctcggcctg tcgggcgcgg
     2761 aaatgggcat gagctacttt ctgccacgca tcgtgggaac cagcgtcgcc gctgagtgga
     2821 tgttgaccgg ccgcaccgtg tccgccgagg aggccgaccg gcgcggcttg gtgagccagc
     2881 tcgtcgcacc cgacgaactg tttgcccggg ctcttgaaat cgcctctgcc attgcacaac
     2941 tcacgccgct gggagtgcag ctgaccaaac gggcactgca ggtcaacacc gacgctgccg
     3001 gcctcacggc ggccctggaa ctcgagaacc gcaaccaggt gctcagccat gccaccgacg
     3061 aagcggccga gcggcggcgg aagtggtcgt cgtgaccggc ccactgcggg gcgtacgcat
     3121 agtgatgatg ggcgggctgg gtcccggccc gttctgcggc atgctgctgg gcgacctggg
     3181 cgctgacgtg ctgcgtgtcg atcagccgcg ccaggtcgac ggcccgctgc ccatcgatta
     3241 caccgtgcgc cgcaatcagc gctcggtcgc gctggacgtg aaacagccac gcggacggca
     3301 actcgtgcat cggctggtcg agaacgccga cgcgttcgtc gacgtgttcc gcccgggcgt
     3361 ggcccaacgg ttgggcatcg ggcccgacga tctgcgcgac gtgaaccgcc ggctggtcta
     3421 cgcgcggatg acgggctacg gccaagacgg gccgtacgcc gaccgcgccg ggcacgacat
     3481 caactacatc gcgctggccg gcgcgctgca cggaatcggc accgaagaca cgcccgtgcc
     3541 gccgctgaac ctcgtcggcg actacggcgg cggcggaatg ctgttggcgg tcgggctgtt
     3601 gagcgcgatc ctcgaggcgc gcgaatccgg ccagggccaa gtgctcgacg tcgcgatggt
     3661 cgacggagcc gccgcgctga tggcgccgta cttcgggatg gtgcccgcag gcacctggcg
     3721 agatcaccgg cacgacaacc ttcttgacgg cgcggcgcac ttctacggca cctacgagac
     3781 ggccgacggt aagcacgtcg cggtcggcgc gttggagcgg cagttctacg ccgagttgtg
     3841 tgagcgcctc ggcgttgatg tcccgcgcgg cgatgacgac ccgtcggcgt ggaaggccca
     3901 ccgcgaaacc ttcgccgcga cgtttcggca gaagacccgc gacgagtggg aacgcgagct
     3961 tggctctccc ggcgtttgtg cggctccagt gctgagcatc agggaggcgc cacaccaccc
     4021 gcacaacgtc gcccgcggat ccttcatcga cgtggacggt gttatgcagc ccgctccggc
     4081 cccgcggttt tcccgaactg ttccgccgcc gcccacgccg ccgggactgg ccggcgacca
     4141 cacccgcgac gtgttgggtg aactggggct ggacgacggt gcggtcgccg agctgatcga
     4201 cgccggcgtg gcacggcagc gatgagcgct tgcgcgaaga gcagacagca gagcagaaag
     4261 gacgactagt ggcttgggac ttctccaccg accccgactg ggccgcgcaa ctgaagtggg
     4321 tcgacgactt cgtgcgttcc gaatgcgaac cgatcgacct gatcgtcaag gaatctcacg
     4381 acctcaacga tcccgtccgc caggcgctga ttccgccgct gcagaagatc gtcaaagacc
     4441 gcggactgtg ggccacgcac ctgggccccc atctcggcgg accgggatac gggcaggtga
     4501 aactcgcgct gctcaacgag attctcggcc gttctgagtg cgcgccaatc gttttcggct
     4561 cgcaagcccc cgactcgggc aatagcgaga tcctcgctca ctacggcaca cccgagctca
     4621 aggagcggta cctggagccg ctgctcgaca accggatcgt gtcgtgtttc tcgatgaccg
     4681 agccgcaggg tggcgccgat ccgaaggtgt tcaccacggc cgccgtacag gacggcgacc
     4741 actggatcat caacggcgag aagtggtttt cgtcgttcgc ctcgatggcc tcgtttctga
     4801 tcgtgatggc catgaccgac ccggacgcgc cgccgtacga gcggtactcg atgttcgtcg
     4861 tgcccgccga gacacccggc atcaacgtgc tgcgcgacgt cgggctcggg taccagccgc
     4921 gcggcggcgg acgcgaaggc tacgtgcgat acgaggacgt gcgcgtgccg gccgatcaca
     4981 tgctcggccc gcgcggcggc gcgttcgtcg tcgcccaaac ccgtctcggc ggcgggcgca
     5041 tccaccacgc catgcggacc gtcgggctgg tgcgccgcat cttcgacatg ctgtgcgagc
     5101 gcgcggtgtc ccggcacacg cagggtgagc tgctcgccaa caagcagctc gtccaggaga
     5161 tggtcgcgga ttcgtggatg gagatcgagg cgttccggct gctgacgctg cagacggcgt
     5221 ggaagatcga ccagcacaac gactacaagg cggtgcgcgc cgacatctcc gcggtcaagg
     5281 cgatgatgca gaaggtgctg cacgacgtgt ccgcgcgcgc gttgcagctg cacggatcgc
     5341 tgggcaccac ccacgagatg ccgttcgtgc aatacctcgt cgagtcgttc gtgctcgggc
     5401 tggccgacgg gcccaccgag gtgcataagg tcacgctggc gcggcttctg ctcaaagacc
     5461 gcaaaccgtc gcccgacgtc ttcccgtcgg aacacctgct gcggctgcgc gcggcggcgg
     5521 aagcgaagtt cgccgacaag ctggccggca tcgaccgccg ctaggccagc caaccgattt
     5581 cgcgcaggaa cggctgggtg tgcgcgatcc ggccggtcag cgtcttcggg tcgccggtgc
     5641 acagcgtcaa cgcggtttgt gtgatgagct cgatgtcctc ggtgtcggtc ttggccaggt
     5701 caagcgtgcc ggcacccggg gtggccaccg ggttcgacgg ccctgcggca ttgaccgcga
     5761 tcccgtcgtc gtacagctcg gccgcaaggc ttttcgtgag acggttcaac gcggccttca
     5821 cggtgccgta gatcccgaac cccgccgccc ggtcgaactc cgaaaacggc gggccctcag
     5881 ggagatcccc gccgaccgag gtgacgttga ggatccaccc gcggccgcgt tcgcgcatac
     5941 ccggaatggc caactgggcc agatgcagcg gcgccagcac gtgcatctcg accatcaggc
     6001 gcacccgccg ctccgggaac gtatcgagcg gacgcaggaa tgtcacggcg gcgttgttga
     6061 ccaagatgtc cggtgcccca acgcgttcga ccacctcgcc gaaaaggcgg tcccggtcgt
     6121 cggtctttga caggtccgcc tgcacggcga ccgccgaccc gccgtcggcg gtgatctcgt
     6181 cgagcgtctg acgcagcgac ccgtggtatt tcgggtcggg gtccatggtg cgggcggtca
     6241 gcgcgacggt cgcgccttcg cgggccaact gctgcgcgat cgccttgcca aggcccctgc
     6301 tggtacccgt aaccaacgcg accttgccgg cgcaact
//
LOCUS       DASWBF010000594         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_580631,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000594 DASWBF010000000
VERSION     DASWBF010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580631"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..543
                     /locus_tag="VF874_22040"
     CDS             400..543
                     /locus_tag="VF874_22040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178646.1"
                     /translation="MQLSELLGPVVLDAAAQRVGTVVDVRLTVDGDLDDNPDLPTMFV
                     LVV"
     gene            566..733
                     /locus_tag="VF874_22045"
     CDS             566..733
                     /locus_tag="VF874_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178647.1"
                     /translation="MGYERSDARRPAMLAALLRWRHRGTFLALWDDVGRVDTKSVSLR
                     AGFHRYSSTLH"
     gene            complement(781..1149)
                     /locus_tag="VF874_22050"
     CDS             complement(781..1149)
                     /locus_tag="VF874_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178648.1"
                     /translation="MSVTAFGDLPLAGRDREWDGDAAEKRVRRWADAQDNPNAKYRDA
                     HVWYDADNKDNFGAYKLLIADVVGGKLQAVPRAVMAAGAVMQGSRGGVDLPKDDIARV
                     KSHLAKYYKKMGDSAPWDRD"
     gene            1247..1990
                     /locus_tag="VF874_22055"
     CDS             1247..1990
                     /locus_tag="VF874_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9178649.1"
                     /translation="MVVRIGTSGWSYDHWAGVLYEPAVPAAGRLARYTAEFGTVELNA
                     SFYRWPRDATFAGWRDRLPAGFLMSVKAARGLTHARRLRSPEEWIERFARCWHELRDR
                     RGALLVQLHPAQERDDARLDYFLGAVPPWMTVAVELRHPSWDDPQVYQLLERRNATYV
                     VMSGAGLPCVLKATAKLVYVRLHGPDPSTLYGGSYSADDLRWWADRIMDWHRGGHDVF
                     AYFNNDGDGNAVRNARDLRSLLRIHGLAS"
     gene            complement(2263..2394)
                     /locus_tag="VF874_22060"
                     /pseudo
     CDS             complement(2263..2394)
                     /locus_tag="VF874_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604278.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT          369 a          790 c          797 g          438 t
ORIGIN      
        1 gttacgctgc ccggcaggaa tcaactggtc atgcatctgc ctctccggcg gcggactccc
       61 actcgctcgc cccatgccca tcctgccgcc aatggatttg tcccgcataa cgtgtggtcc
      121 caaaacggtc ccaaagcaag cgctcgtaac cctggggaag ttgggcggcc ggactagagc
      181 cgtgcggacg agtaattggc ggtggatcgg cagctgccac cagggccgtg ctcgtccgtc
      241 ggggcaaaag ccgttgtgcg tgaagcacaa gaacgctggc gctcggcgct ccggtcaccg
      301 cggcggtcgc acccccggta cgcgcgggca gcgtatggga ctgtcgaaag cgcggcttgc
      361 tgcgcgatca catcatcggc cgcatccctg gagggtgccg tgcagctgag tgaactcctc
      421 gggcccgtcg tgctggacgc cgccgctcag cgcgtcggca ccgtcgtcga cgtcaggctg
      481 accgtggacg gcgacctcga tgacaaccct gatctcccaa cgatgttcgt tttggttgtc
      541 tagcccgcgg acacgctcgt cctacttggg gtatgaacgc tccgacgccc gccgacccgc
      601 gatgctggcc gcgctgctgc gctggcgcca ccgcggcacc ttcctagcgt tgtgggacga
      661 cgtcggccga gtcgacacga agtcggtgtc ccttcgggcc gggttccacc gctactcatc
      721 gaccttgcac taacgcaagc accggttcgt tcgcgcagag tacgggcaaa gtccagcgac
      781 ctaatcccgg tcccaggggg cgctgtcccc catcttcttg tagtacttgg cgaggtggct
      841 tttcacgcgt gcaatgtcgt ctttgggtag gtcgacgccg ccgcgcgagc cctgcatcac
      901 agcgccggcg gccatcactg cgcgcggcac tgcctgaagt ttcccgccga ccacatcggc
      961 gataagcagc ttgtaggcgc cgaaattgtc cttgttgtcg gcgtcgtacc agacgtgcgc
     1021 gtcgcggtac ttcgcgttgg ggttgtcttg cgcgtcggcc catcgacgga cgcgtttttc
     1081 ggccgcgtcg ccatcccatt cccgatcccg accagccagc ggcagatccc cgaatgcggt
     1141 caccgacatg atgtctcctc gctcactgcg gggcgggtgc ccgccgccag tgagagttaa
     1201 acgcctgtga ggagtggtgc gcacaagacg cgggtacatc ggttccatgg tggtcaggat
     1261 cggcacgtca ggctggtcct acgaccactg ggccggtgtg ctctacgagc cggccgtgcc
     1321 agcagcaggc cggcttgcgc gttataccgc ggaattcggc accgtcgagc tgaacgccag
     1381 cttctaccga tggccccgcg acgcgacgtt cgcgggatgg cgggaccggc taccggcagg
     1441 ctttctgatg tcggtgaagg cggcgcgggg tctcacccat gcgcgacgtc tgcgttcccc
     1501 cgaggagtgg atcgaacgct tcgctcggtg ctggcacgag ctgagggatc ggcgcggcgc
     1561 gctgctggtg cagctacacc ccgcgcagga gcgcgacgat gcccggctgg actacttcct
     1621 cggagcggta ccgccgtgga tgacggtcgc ggtggagttg cgacatccct cctgggacga
     1681 cccgcaggtg tatcagctgc ttgagcggcg caatgcgacg tatgtcgtca tgagtggcgc
     1741 gggtctgccg tgcgttctga aggcgaccgc gaagctggtc tacgtgcggc tgcacggtcc
     1801 cgatccgtcc accctctacg gaggctccta cagcgccgac gacctgcggt ggtgggctga
     1861 tcggatcatg gattggcatc gcggcggcca cgatgtgttc gcgtacttca acaatgacgg
     1921 tgacggcaac gctgtgcgca acgcccggga cctgcgctcc cttcttcgca tccacggtct
     1981 ggcaagctga gcggccacgc tctggccgga caatgctgcc acgcaacatc tttcacggcc
     2041 atggatgcgt gggcgctgtg gttgaatcac tgcccggccg ggtatcacca gcaggccgca
     2101 ccagaggcgg ccggaatcgg gaggtcgcca tgagccgacg ctcgaaggcg ttgtacatgc
     2161 cgctgtccat tgctaccagc gtggccggtg gctgactcgc cggggcggtg ttcagtcaga
     2221 tctggaaacg tctcggcgat gccgatcagc cccgccggat ctccggcggt ccttgccctt
     2281 gccgcggacg tggatgacgg cgccatcgtg gctgcgccga aggtcaccga cgttggcgcg
     2341 caccagttca tcggcgcgca acccggccag cagcgcggtc aggatcaggg cgcg
//
LOCUS       DASWBF010000595        20776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1115898,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000595 DASWBF010000000
VERSION     DASWBF010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20776
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115898"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF874_22065"
     CDS             complement(<1..387)
                     /locus_tag="VF874_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9178650.1"
                     /translation="MTVVTDTYTGHVDQGTAARRTLPGATIIKASVGPMDNNAYLITC
                     SETGESLLIDAANDADDLVDLVRQYAPKLALIVTSHQHWDHWQALEAVVDATGAPTAV
                     HEIDADPLPVKPDRLLADGDTVEVGKL"
     gene            459..3344
                     /gene="uvrA"
                     /locus_tag="VF874_22070"
     CDS             459..3344
                     /gene="uvrA"
                     /locus_tag="VF874_22070"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9178651.1"
                     /translation="MAERLIIKGAREHNLRSVDLDLPRNALIVFTGLSGSGKSSLAFD
                     TIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGTI
                     TEVYDYLRLLYARAGTPHCPVCGERIARQTPQQIVDQVLAMPEGVRFLVLAPVVRTRK
                     GEFADLFEKLNSQGYSRVRVDGVVHSLADPPTLKKQEKHDIEVVVDRLTVKPTAKQRL
                     TDSVETALRLADGIVVLEFVDHEHDHPHREQRFSEKLACPNGHPLAVDDLEPRSFSFN
                     SPYGACPECVGLGIRKEVDPDLVVPDPDLTLAEGAVAPWSNGHTAEYFTRMMASLGEQ
                     MGFDVDTPWCKLPAKARKAILEGCDEQVHVRYRNRYGRTRSYYADFEGVMAFLQRKME
                     QTDSETMKERYEGFMRDVPCPECQGTRLKPEILAVTLSAGERGAMSIAEVSELCISDC
                     AEFLNALTLGPREEAIAGQVLKEIQSRLGFLLDVGLEYLSLSRAAATLSGGEAQRIRL
                     ATQIGSGLVGVLYVLDEPSIGLHQRDNRRLIETLLRLRDLGNTLIVVEHDLDTIAHAD
                     WVVDIGPAAGEHGGQIVHSGTYDDLLRNKNSITGAYLSGKESIEVPPIRRPVDRRRQL
                     TVVGAREHNLRDIDVSFPLGVLTSVTGVSGSGKSTLVNDILAAVLANKLNGARQVPGR
                     HTRVTGLDKVDKLVRVDQSPIGRTPRSNPATYTGVFDKIRTLFAATTEAKVRGYQPGR
                     FSFNVKGGRCEACTGDGTIKIEMNFLPDVYVPCEVCHGARYNRETLEVHYKGKTVSEV
                     LDMSIEEAAEFFEPITSIHRYLQTLVDVGLGYVRLGQPAPTLSGGEAQRVKLAAELQK
                     RSTGRTVYILDEPTTGLHFEDIRKLLGVINGLVDKGNTVIVIEHNLDVIKTSDWIVDM
                     GPEGGAAGGTVVAQGTPEDVAAVPESYTGQFLADPLGLRRRRGRVSA"
     gene            complement(3433..3699)
                     /locus_tag="VF874_22075"
     CDS             complement(3433..3699)
                     /locus_tag="VF874_22075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178652.1"
                     /translation="MPDEPTPPEPKPVTPDVEPGYNESGVPTFESVREKIETRYGTSL
                     GAAELDAETPEGRTVEEQYEARQRAAAERLAEIRKSMQSGQGDT"
     gene            complement(3720..5081)
                     /locus_tag="VF874_22080"
     CDS             complement(3720..5081)
                     /locus_tag="VF874_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9178653.1"
                     /translation="MHGWVPAIVQATAVTVLLLAIGWRSRRWRMVCLPLAVLVGGATA
                     ATGRWYIDSQGLAGDPPPRSLLIWIGLTGMAVGVLILGWRRVRWRRRSVAMSAVPLCL
                     LCAALALNQWVGYFPTVQTAWNQMTAGSLPDQTDRATVTAMQRNGRKPVTGTVVPVDT
                     GDSGSRFKHRGELVYLPPAWYATDPPPRLPTVMMIGGEFNTPADWLRAGNAIKTIDDF
                     AAAHGGNAPVFVFVDSGGAFNNDTECVNGPRGNAADHLTKDVVPFMVSNFGVSTSPSN
                     WGIAGWSMGGTCALDLTVMHSDMFSAFEDIAGDMSPNSGNKTQTIDRLFGGNAAAWAS
                     FDPSTVITRHGRYTGVTGWFAINSGQQSQPHVMPAANRVTPNTTGDQTAAANTLCGLG
                     SAHGIDCAVVAQAGKHDWPFAARAFAAALPWLAGQLRTPGVPHVPFPSPAPTAQVVQA
                     AGR"
     gene            complement(5146..8445)
                     /gene="lysX"
                     /locus_tag="VF874_22085"
     CDS             complement(5146..8445)
                     /gene="lysX"
                     /locus_tag="VF874_22085"
                     /EC_number="2.3.2.3"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     synthetase/lysine--tRNA ligase LysX"
                     /protein_id="HEX9178654.1"
                     /translation="MTLTTARPTSRFSWVPATAGWVVGIIATLSLLASVSPLMRWIIR
                     VPREFIDDYIFNFPDTSFAWAFVLALLAAALAARKRIAWWLLVLNLVFAAGWNVGDIA
                     SDTENRLETFGELVGLAVHVGAVVLLLLSYREFYARVRRGALLKSTGVLVFGMAVGTL
                     LAWGLVEAFPGTLPRDQRLPYALNRVSGFALADPDLFTGHPHVLLNTLFGLFGAVALM
                     AAAVVLFQSQRAENALTGEDESAIRGLLEVYGKNDSLGYFATRRDKAVVFADNARAAI
                     TYRVEVGVCLASGDPVGDPRSWPAAIEAWLRLCQKYGWAPGVMGASIDGAAAYRATGL
                     NALQLGDEAILYPDKFRLSGPSMRTVRQAVTRARRAGLTVQIRRHSDISPDEMTKVIT
                     RADNWRDTETERGFSMALGRLGDSADGDCLLVEAVNGNDDVVAMLSLVPWGSTGVSLD
                     LMRRSPQSPNGTIELMVSELNMQAERFGINRISLNFAMFRSAFEEGARLGAGPIARLW
                     RALLVFFSRWWQLETLYRSNMKYQPHWVPRYACYEDARVIPRVGVASVIAEGFLVLPF
                     SRRKKPHTGHHPAVPENVIATGALHHDGTTPDVAALPDEAAASEDVALRVPDQVKVRM
                     AKLAGLQADGVDPYPVGCAPSHTIADTIDESDGTTVTVAGRVLRVRDYGGVLFAHLRD
                     WSADIQLLLDNSALDDGSTADFTAAIDLGDLIEVAGTMGYSKNGTRSLIVRSWRLIGK
                     CLRPLPDKWKGLTDPEARVRARYVDLAINREARDLITARSQVLHAIRETLVGKGFIEV
                     ETPILQQIHGGANARPFLTHINAYDLGLYLRIAPELYLKRLCVGGVERVFELGRAFRN
                     EGVDFSHNPEFTLLEAYQAHADYMTWIGGCRELIQNAALAANGECVVMRPGDDGSLVP
                     VDISGTWDVKTVHGAVSDALGEQIDADTDLATLRRLCDEADIPYLTQWDAGAVVLELY
                     ERLVEERTEQPTFYKDFPTSVSPLTRPHRSKAGLAERWDLVAWGIELGTAYSELTDPV
                     EQRRRLQEQSLLAAGGDPEAMELDEDFLKAMEYAMPPTGGLGMGVDRVVMLITGRSIR
                     ETLPFPLAKPR"
     gene            8814..9407
                     /gene="infC"
                     /locus_tag="VF874_22090"
     CDS             8814..9407
                     /gene="infC"
                     /locus_tag="VF874_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX9178655.1"
                     /translation="MTTETRVNERIRVPEVRLIGPGGEQVGIVRIEDALRVAADADLD
                     LVEVAPNARPPVCKIMDYGKFKYETAQKARESRKNQQQTVVKEQKLRPKIDNHDYETK
                     KGHVIRFLEAGSKVKVTIMFRGREQSRPELGYRLLQRLGADVADYGFVETSAKQDGRN
                     MTMVLAPHRGAKTRARAAQQASGPPNGQPDSTDTSQN"
     gene            9414..9608
                     /gene="rpmI"
                     /locus_tag="VF874_22095"
     CDS             9414..9608
                     /gene="rpmI"
                     /locus_tag="VF874_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX9178656.1"
                     /translation="MPKAKTHSGASKRFRRTGTGKILRQRANRRHLLEHKPAKRTRRL
                     EGRVTVSTKDARRIRKLLNG"
     gene            9656..10051
                     /gene="rplT"
                     /locus_tag="VF874_22100"
     CDS             9656..10051
                     /gene="rplT"
                     /locus_tag="VF874_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX9178657.1"
                     /translation="MARVKRAVNAHKKRRTILKASKGYRGQRSRLYRKAKEQQLHSLN
                     YAYRDRRARKGEFRKLWISRINAAARANDITYNRLIQGLKAAGVEVDRKNLAEIAVSD
                     PGAFTALVGVARAALPEDVNAPSNSGTAA"
     gene            10054..10839
                     /locus_tag="VF874_22105"
     CDS             10054..10839
                     /locus_tag="VF874_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9178658.1"
                     /translation="MALTERSARVVAAAKLHRHVARTRAGRFLAEGPNLVEAALRCGV
                     ARELFVTGSAATRFDALLADVDVPVHLVTERAANALSDTVTPVGLVAVCEPPTVTLAD
                     VVAARPKLVVVAVAVSDPGNAGTLIRLADAMGAGAVVLSGHSVDPYNAKCLRASAGSV
                     FAVPVVPDTDPAAVVGALAEAGLQVLATTVDGETLLDDIEPLLSAPTAWLFGSEAHGL
                     PEEVATLADRRVRIPLDGGAESLNVAAAAAICLYSSARAQRQG"
     gene            complement(10840..10977)
                     /locus_tag="VF874_22110"
                     /pseudo
     CDS             complement(10840..10977)
                     /locus_tag="VF874_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11173..12093
                     /locus_tag="VF874_22115"
     CDS             11173..12093
                     /locus_tag="VF874_22115"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9178659.1"
                     /translation="MKVEPSGDRSGGLGEVDESPDDRPEQSPEQRGAAAWLTNTNRNP
                     GVIAAIRRARKALPGDPHFGDRLSASGFGGPAAAARAADRLLGDRDAASRELSLGALQ
                     VWQAVSERVIRKPTQDEVTLVFTDLVGFSQWALQAGDDATLKLLRQVAQVVEPPLLEV
                     GGQVVKRMGDGIMAVFPDPVTAIAAICAATDALKNVDVEGYTPVMRVGIHTGRPQRIG
                     SDWLGVDVNIAARVMECAGKGGVLVSGATLERLTPEQLDELGAYAKRLRRPMFAHRLS
                     GVPPDITMYRLESRKDLTAADTTKDSPSQE"
     gene            12102..12914
                     /locus_tag="VF874_22120"
     CDS             12102..12914
                     /locus_tag="VF874_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
                     /protein_id="HEX9178660.1"
                     /translation="MVTGALCTLFRLRVTVSYAVMLVAVATTLLMLGPGVRELVISRM
                     STNLHNLEHGRLGTLIGSAFVTENGPIWVWLPGLVCLLALAELLWRSGRVLVTFAIGH
                     VGATLIVTAGLAAAIHVGWLPISVARASDVGISYGAVAVLGALTAAIPSRWRPAWIGW
                     WLAVGLLVVCAGVVGAGGGEFTDAGHVVALLLGILLSLRFGAVTRWTRVRLALLVIGV
                     AFGYLVLVNTGLPVILAPIVGTIGALAAHWLAGRWRARGVLPAAAAAAAVTT"
     gene            12945..13988
                     /gene="pheS"
                     /locus_tag="VF874_22125"
     CDS             12945..13988
                     /gene="pheS"
                     /locus_tag="VF874_22125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9178661.1"
                     /translation="MADQPVDLSEEALTKAVSAARHAFGLATDLDGLARAKSEHLGDR
                     SAVALARQALGALPKADRADAGRRVNVARTEVQHAYDERLAALRAERNAAVLVAERVD
                     VTLPSTRQPIGARHPITILAEHVADTFVAMGWELAEGPELETEQFNFDALNFPPDHPA
                     RSEQDTFQVAPDGSRQVLRTHTSPVQIRALLERELPVYIVSIGRTFRTDELDSTHTPV
                     FHQVEGLAVDRGLTMAHLRGTLDAFARAEFGPQGRTRFRPHFFPFTEPSAEVDVWFAG
                     KKGGPGWVEWGGCGMVHPNVLRACGIDPEQYSGFAFGMGLERTLQFRNGIPDMRDMVE
                     GDVRFSLPFGVGA"
     gene            13988..16480
                     /gene="pheT"
                     /locus_tag="VF874_22130"
     CDS             13988..16480
                     /gene="pheT"
                     /locus_tag="VF874_22130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9178662.1"
                     /translation="MRVPYSWLREVVQAGAPGWDVSPDELEQTLIRIGHEVEEVIAVG
                     PVAGPLTVGRVLEVEELTEFKKPIRAVKVDVGETQPRDIVCGATNFVVGDLVAVALPG
                     TVLPGDFTITTRKTYGRTSDGMISSAAELNLGVDHSGILVLPDGAASPGDDAAPVLGL
                     GLGDVVFHLAITPDRGYCLSVRGLAREIACACDLDFVDPADVPALPAEGEAWPLTIEP
                     GTGVRRFALRPVTGIDPAAVSPWWMQRRLLLSGMRPISPAVDVTNYVMLELGHPMHAH
                     DLKRITGGFRVRFARPGETVVTLDDIERHLNPDDVLIVDDVGTAAIGGVMGAGTTEMG
                     DDSTDVLLEAAVWDPAAVSRTQRRLHLPSESARRYERSVDPAISVAALDRASVLLAEI
                     ASGTIEPKLTDWRGDPSRDDWSMPPIDMPADLPDRTAGVRYPDGATRRRLEQVGTAVT
                     ADDGTLTVIPPSWRPDLRQPADLVEEILRLAGLDLIPSVLPLAPPGRGLTAGQKRRRA
                     IGKSLALSGYVEVLPTPFLPAAVFDQWGLPADDPRRNTMKVLNPLEADRPELATTLLP
                     GLLEALSRNVSRGTVDVSLYAIEQVVQPTERTRAVEPLPVDRRPTHEEIVVLDDSLPR
                     QPQHVAAVMAGLREPRGPWGPGRAVEAADAFEVVRTVARASGVELTLRVAQHLPWHPG
                     RCAEVLVGETVIGHAGQLHPAVVERSGLPKGTCAVELDLDAIPITEAMPAPRVSPFPA
                     VFQDVSLVVADDVGAQAVIDAVRDGAGELLEDVRLFDVYTGPQIGERRKSVTLALRFR
                     AADRTLTEDAASAARDAAVRRAAELVGAVQRA"
     gene            16612..17655
                     /gene="argC"
                     /locus_tag="VF874_22135"
     CDS             16612..17655
                     /gene="argC"
                     /locus_tag="VF874_22135"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX9178663.1"
                     /translation="MAAMTTVAVAGASGYAGGEILRLLLGHPAYADGRLTIGALTGAT
                     SAGSTVAEHHPHLLPLASRVLESTEARVLSGHDVVFLALPHGHSAALAEQLDADTLIV
                     DCGADFRLTAANVWEKYYDSPHAGTWPYGLPELPGNRDRLRGARRIAVPGCYPTAALL
                     ALLPAVAENLIEPVVTVVAVSGTSGAGRTAKPELLGSEVIGSARAYNIGGVHRHTPEI
                     AQGLQALTDRAVHVSFTPVLIPMSRGILATCTARTATPLSRLREAYEKAYASEPFVHL
                     MPEGQLPRTGAVMGSNAAQVAIAVDDDAGMFVAVCAIDNLVKGTGGAAVQSMNLALGW
                     PEGEGLSVVGVAP"
     gene            17652..18866
                     /gene="argJ"
                     /locus_tag="VF874_22140"
     CDS             17652..18866
                     /gene="argJ"
                     /locus_tag="VF874_22140"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX9178664.1"
                     /translation="MTGVSAQTRLVRTQGVTAPAGFRAAGIAAGIKASGNLDLALVFN
                     EGPDYHAAGVFTRNQVKAAPVLWTQQVMTTGRLRAVILNSGGANACTGHQGFQDTHAT
                     AEAVAEALSDWGTETGAIEVAVCSTGLIGDRLPMDKVLSGVRDVVHDLAGGLSGGEEA
                     ARAIMTTDTVPKQVALHHGGNWTVGGMAKGAGMLAPSLATMLCAITTDAVADARALDA
                     ALRRAVALTFDRLDVDGSCSTNDTILLMSSGASEITPSQADLDDALLQVCDDLVAQLQ
                     ADAEGVTKRVTITVTGAATENDALTVARVIARDSLVKTAMFGSDPNWGRVVAAVGMGP
                     VRLDPDRITVSFNDFPVCVDGVGAPGARTVDLSGPYIHMTVDIGVGSEQASIRTTDLS
                     HGYVEENSAYSS"
     gene            18863..19744
                     /gene="argB"
                     /locus_tag="VF874_22145"
     CDS             18863..19744
                     /gene="argB"
                     /locus_tag="VF874_22145"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX9178665.1"
                     /translation="MSAPAVTAHVKAQVLAEALPWLKALNDKIVVVKYGGNAMIDDAL
                     RQAFAADMVFLRNCGIHPVVVHGGGPQITAMLKRLGIAGDFKAGFRVTTPEVLDVARM
                     VLFGQVGRELVNQINAHGPYAVGITGEDAHLFTAVRRRVTVDGVDTDIGLVGDVATVN
                     TAAVLDLIAAGRIPVVSSIAPDADGIVHNINADTAAAALAEALEAEKLLMLTDVEGLY
                     ARWPDRDSFVSEIDTETLSELLPSLDAGMVPKVEACLRAVAAGVPSAHVIDGRVDHCV
                     LVELLTDAGTGTKVVRA"
     gene            19741..>20776
                     /locus_tag="VF874_22150"
     CDS             19741..>20776
                     /locus_tag="VF874_22150"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX9178666.1"
                     /translation="MTLEERWAAVMMDNYGTPPLALASGDGAVVTDVDGNRYLDLLGG
                     IAVNVLGHRHPAVIDAVTRQLNTLGHTSNLYLHEPGIALAEVLVGLLGADAETRVFFC
                     NSGAEANEVAFKLSRLTGRTKLVAAQEAFHGRTMGALALTGQPSKQAPFAPLPGEVTH
                     VPYGDADALAQAVSDDTAAVFLEPIMGESGVVVPPAGYLAAAREITARHGALLVLDEV
                     QTGMGRTGVFFAHQHDGVTPDVVTMAKGLGGGLPIGACLAVGPAARLMTPGLHGSTFG
                     GNPVCAAAALAVLRTITEQDLIGRAGVLGKTLSHGIESMNHPLVDHVRGRGLLRGVVL
                     TSPVAKPVEAA"
BASE COUNT         3390 a         6899 c         7118 g         3369 t
ORIGIN      
        1 cagcttgccg acctcgactg tgtcgccgtc ggccagcagc cggtccggtt tcaccggcag
       61 cgggtcggcg tcgatctcgt gcactgcggt cggggcgccg gtcgcgtcga cgacggcctc
      121 cagcgcctgc cagtggtccc agtgctggtg gctggtgacg atcagcgcca gcttcggcgc
      181 gtactggcgg acgagatcga cgaggtcgtc ggcgtcgttg gcggcgtcga tcagcagcga
      241 ctcgccggtc tcggaacatg tgatcaggta ggcgttgttg tccatggggc ccaccgaggc
      301 cttgatgatc gtcgcgccgg ggagagtgcg gcgcgcagcg gtgccctgat cgacgtggcc
      361 ggtgtaagtg tctgtcacga ctgtcatgtg gccaacgcta gcgtgtcggt gggcacacat
      421 agcatggtca aggactgcgt ctttctggaa ggaaatgggt ggctgagcgc ctgatcatca
      481 agggtgcgcg tgagcacaac ttgcgaagcg tcgacctcga cttgccccgc aatgcgctga
      541 tcgtgttcac cgggctgtcc gggtcgggca agtcgtcgct ggccttcgac accatcttcg
      601 ccgagggcca gcgccgctac gtcgagtcgc tgtcggcgta cgcgcggcag ttcctcggcc
      661 agatggacaa gccggacgtc gacttcatcg aggggctgtc gccggcggtc tccatcgacc
      721 agaagtccac caaccgcaac ccgcgctcga ccgtgggcac gatcaccgag gtctacgact
      781 acctccggtt gctctatgcc cgcgccggca ccccgcactg cccggtgtgc ggcgagcgga
      841 tcgcccgcca gactccgcag cagatcgtcg accaggtgct cgcgatgccc gagggggtgc
      901 gctttttggt gctggcgccg gtggtccgca cccgcaaggg cgagttcgcg gacctgttcg
      961 agaagctgaa ttcgcagggc tacagccggg tccgggtgga cggtgtcgtc cattcgctcg
     1021 ccgatccgcc gacgctgaag aagcaggaga agcacgacat cgaggtggtg gtcgaccggc
     1081 tcaccgtgaa gccgacggcc aagcagcggc tcaccgactc ggtggaaacc gcgctgcggc
     1141 ttgccgacgg gatcgtcgtg ctcgagttcg tcgaccacga gcatgatcac ccgcaccgcg
     1201 agcagcggtt ctcggagaag ctggcctgcc ccaacggcca tccgctggcg gtagacgacc
     1261 ttgagccacg gtcgttttcg ttcaactcgc cgtacggcgc gtgccccgag tgcgtcgggc
     1321 tgggcatccg caaggaggtc gacccggacc tcgtcgtgcc cgatcccgac ctgacgctgg
     1381 ccgaaggcgc ggtggcgccc tggtccaacg ggcacaccgc ggaatacttc accaggatga
     1441 tggccagcct cggcgagcag atgggcttcg acgtcgacac cccgtggtgc aagcttccgg
     1501 ccaaggcgcg caaggcaatc ctggagggct gcgacgagca ggtgcacgtc cgctaccgca
     1561 accggtacgg gcgcaccagg tcgtactacg ccgacttcga aggtgtgatg gcgttcttgc
     1621 agcgcaagat ggagcagacc gactccgaga caatgaagga gcggtacgag ggcttcatgc
     1681 gcgacgtgcc gtgcccggaa tgccagggta cccggctgaa gccggagatt ctggcggtga
     1741 cgttgtccgc gggggagcgc ggcgcgatgt cgatcgccga ggtttccgag ttgtgcatct
     1801 ccgactgcgc cgagttcctg aacgctttga cgctcggccc gcgagaagag gcgatcgccg
     1861 gtcaggtgct caaggagatc cagtcgcggc tcgggttcct gctcgacgtg gggttggagt
     1921 acctgtcgtt gtcaagggcg gcggccacgc tgtccggcgg tgaggcgcaa cggattcggc
     1981 tcgcgacgca gatcggctcc gggttggtcg gcgtgctgta tgtgctcgac gagccgtcga
     2041 tcgggctgca tcagcgcgac aaccgcagac tgatcgagac gctactgcgg ctgcgggatc
     2101 tgggtaacac gctgatcgtc gtcgagcacg acctcgacac catcgcccac gccgactggg
     2161 tcgtcgacat cggccccgca gcaggagagc acggcggtca gatcgtgcac agcggcacgt
     2221 acgacgacct tctgcgcaac aagaattcga tcacgggcgc ctatctttcg ggcaaggaga
     2281 gcatcgaggt tcccccgatc cgccggccgg tggaccgccg tcggcaactg accgtggtcg
     2341 gcgcccgcga gcacaacctg cgcgacatcg acgtgtcgtt cccgctcggg gtgctgacgt
     2401 cggtcaccgg cgtctccggc tcgggcaagt ccacgctggt gaacgacatc ctcgcggcgg
     2461 tgctggccaa caagctcaac ggcgcgcgtc aggtgcccgg ccggcacacg cgagttaccg
     2521 gcctggacaa ggtggacaag ctggtgcgcg tcgaccagtc accgattggg cgcaccccgc
     2581 gatcgaaccc ggccacctac accggggtgt tcgacaagat ccgcacattg ttcgcggcca
     2641 ccaccgaggc gaaagtccgt ggctatcaac cgggtcggtt ctcgttcaac gtcaagggcg
     2701 gccgctgcga ggcgtgcacg ggcgacggca cgatcaagat cgagatgaac ttcctgcccg
     2761 acgtgtacgt gccctgcgag gtgtgccacg gcgcccgcta caaccgcgag accctcgagg
     2821 tgcactacaa gggcaagacc gtctccgagg ttctggacat gtcgatcgag gaggccgccg
     2881 agttcttcga gccgatcacg tcgatccacc ggtatctgca gacgctggtc gacgtcggtc
     2941 tgggctatgt gcggctcggg cagccggcac ccaccctgtc cggcggcgag gcgcagcgcg
     3001 tgaagctggc cgcagaactg cagaagcgct caactggccg gaccgtgtac atcctcgacg
     3061 agcccaccac cggcctgcac ttcgaggaca tccgcaagct gctgggcgtc atcaacggcc
     3121 ttgtggacaa aggcaatacg gtcatcgtga tcgagcacaa cctcgacgtc atcaagacgt
     3181 ctgactggat cgtcgacatg ggcccggaag gcggagcggc cggcggcacg gtggtcgccc
     3241 agggcacacc ggaagacgtt gcggccgtgc cggagagcta cacgggtcaa ttcctggccg
     3301 acccgctagg actgcggcgc cgccgtggcc gggtcagcgc gtagttcacc gaacgtgagc
     3361 ccaccgcgaa aattcggccg gattttcgca gtagcttcac gctcgcggcg gcacaccagc
     3421 ttttgagccg ggttacgtgt cgccctgccc ggattgcatc gacttgcgaa tttccgcgag
     3481 acgttcggcc gcagcgcgtt gacgtgcctc gtactgttct tcgaccgtgc ggccctcggg
     3541 ggtttcggcg tcgagctccg ctgcaccgag tgaggtgccg taccgagtct cgatcttttc
     3601 gcgcacagac tcgaacgtcg gtactccgga ctcgttgtac ccaggttcga cgtccggcgt
     3661 gaccggcttg ggttcaggag gcgtcggctc gtcgggcatg ccgccaccgt actcctgctt
     3721 cacctgccgg cggcctgaac cacctgcgct gtcggagcgg gtgacgggaa cgggacatgc
     3781 ggcacaccgg gcgttcggag ctgcccggcc agccaaggca gggcagccgc gaaagccctt
     3841 gccgcgaacg gccagtcgtg cttgcccgcc tgtgccacca ccgcgcagtc gatgccgtgg
     3901 gcgctgccca gcccgcagag cgtgttcgcg gcggcggtct ggtcgccggt ggtgttgggt
     3961 gtgacgcggt ttgcggccgg catcacatgc ggctgggact gctggccgct gttgatcgcg
     4021 aaccagcctg tgacgccggt gtacctcccg tggcgcgtga tcactgtcga cgggtcgaaa
     4081 gacgcccagg cggcggcgtt tccaccgaag agccgatcga tggtctgcgt cttgtttcct
     4141 gaattcggac tcatgtcgcc ggcgatgtct tcgaatgcac tgaacatgtc cgagtgcatc
     4201 acggtcaggt cgagtgcgca ggtaccgccc atcgaccatc ccgcgatgcc ccagttggat
     4261 gggctggtgc tgactccgaa gttcgagacc atgaacggga caacatcttt cgtcaggtgg
     4321 tctgccgcgt tgccgcgagg cccgttcacg cattccgtgt cgttgttgaa cgcgccacca
     4381 gagtcgacga acacgaaaac cggtgcgttg ccgccgtgcg cggccgcgaa gtcatcgatg
     4441 gttttgatgg cgttgccggc acgcagccag tcggcgggcg tgttgaactc gccgccgatc
     4501 atcatcaccg tcggcaaccg gggtggggga tctgtggcat accaggccgg cgggaggtac
     4561 accagttccc cgcgatgctt gaacctcgag ccggagtcgc cggtgtccac cggtacgacg
     4621 gttcccgtga ccggcttcct gccgttgcgc tgcatcgcgg tgacggtcgc ccggtcggtc
     4681 tggtccggaa gcgagccggc cgtcatctga ttccaggccg tctgcacggt ggggaagtag
     4741 ccgacccact gattcagcgc cagcgccgcg cacagcagac acaacggcac cgcagacatc
     4801 gcaacactgc ggcgtcgcca gcgcactcgg cgccagccga gaatcagcac gccgaccgcc
     4861 atgccggtca acccgatcca gatcaacagc gagcgcggcg gggggtcgcc cgcaagacct
     4921 tgcgaatcga tgtaccagcg gcccgtcgcc gctgtcgccc caccgaccag tactgccagc
     4981 ggcaagcaca ccatccgcca ccgtcgcgac cgccacccga ttgccagcag cagcacggtc
     5041 accgcggtgg cctgcacgat cgctggcacc cacccgtgca tcagcgagat gtggtggtgg
     5101 atcacacaac tatcgtgtat cgagaacctg cgcgttgcct gtgagtcagc ggggcttggc
     5161 caacgggaac ggcagtgtct cccggatgct gcggccggtg atcagcatga ccacccggtc
     5221 gacgcccatg ccgagcccgc cggtgggtgg catcgcgtac tccatcgctt tcaggaagtc
     5281 ctcgtcgagt tccatcgctt cggggtcgcc gccggccgcc aatagcgact gctcctgcag
     5341 ccgacggcgc tgctcgaccg ggtcggtcaa ctcgctgtag gccgtgccga gttcgatgcc
     5401 ccatgcgacc aggtcccagc gttcggccaa ccccgccttg ctgcggtgcg ggcgggtcag
     5461 cggtgacacc gacgtcggga agtccttgta gaacgtgggc tgctcggtgc gctcttcgac
     5521 gagccgctcg tagagttcga gcaccaccgc gccggcgtcc cactgcgtca ggtaggggat
     5581 gtcggcctcg tcgcacagcc gtcgcaacgt cgcaaggtcg gtgtcggcgt cgatctgctc
     5641 gccgagcgcg tccgacaccg ccccgtgcac ggtcttgacg tcccaggtgc cggagatgtc
     5701 caccggaacc agcgagccgt cgtctcccgg tcgcatcacg acgcactcgc cgttggccgc
     5761 cagcgccgcg ttctggatca gctcgcggca tccgccgatc cacgtcatgt agtcggcgtg
     5821 tgcctggtag gcctccagca acgtgaactc cggattgtgg ctgaagtcga cgccttcgtt
     5881 gcggaacgcc cggccaagct cgaacacgcg ctccaccccg ccgacgcaca ggcgcttgag
     5941 gtacagctcc ggcgcgatac gcagatagag gccgagatcg taggcgttga tgtgggtgag
     6001 aaacggccgg gcgttggcgc cgccgtggat ctgctgcagg atcggcgttt cgacctcgat
     6061 aaaacccttg cctaccaatg tttctcggat ggcgtgcagc acctggctgc gtgccgtgat
     6121 gagatcgcgg gcctcgcggt tgatcgccag gtcgacgtag cgggcacgga cccgcgcttc
     6181 cgggtcggtg aggcccttcc acttgtccgg tagcggccgc aggcacttgc cgatcagccg
     6241 ccagctgcgc acgatcagcg accgggtgcc gttcttgctg tatcccatgg tgcctgcgac
     6301 ttcgatgagg tcacccaaat cgatcgccgc ggtgaagtcg gccgtcgacc cgtcgtcaag
     6361 cgctgaatta tcaaggagca gttggatatc cgcggaccag tcgcgcaggt gggcgaacag
     6421 caccccgccg tagtcgcgga cgcgcagcac acgcccggcc accgtcacgg tggtgccgtc
     6481 gctttcgtcg atcgtgtcgg cgatcgtgtg gctgggcgcg cagccgacgg ggtaggggtc
     6541 gacgccgtcg gcttgaagcc cggccagctt ggccatccgc accttgacct gatccggtac
     6601 ccgcagcgcg acgtcctccg acgcggcggc ttcgtccggt agtgccgcga cgtcgggcgt
     6661 ggtgccgtcg tggtgcaacg ccccggtggc tatgacgttc tcgggcaccg ccgggtggtg
     6721 gccggtgtgt ggctttttgc gccgcgagaa cggcaacacc aggaaaccct cggcaatcac
     6781 agacgccaca cccacccgag gaatcacccg ggcgtcctcg tagcaggcat accgcggcac
     6841 ccagtgcggc tgatacttca tgttggaccg gtacagcgtt tccagctgcc accaccgcga
     6901 gaagaacacc agcagtgcac gccacagccg ggcaatcggg ccggccccca gtctggcacc
     6961 ctcttcgaac gccgaccgga acatcgcgaa gttcaacgaa atgcggttga tcccaaagcg
     7021 ttccgcttgc atgttgagtt cgctgaccat cagttcgatg gttccgttcg gggactgcgg
     7081 tgagcggcgc atcaagtcca gcgacacccc ggtgctgccc cagggcacca gcgacagcat
     7141 cgccaccacg tcgtcgttgc cgttcacggc ttccacgagc agacagtcgc cgtcagcgct
     7201 gtcccccagg cggcccagcg ccatcgagaa cccgcgctcg gtctcggtgt cgcgccagtt
     7261 gtcagcgcgc gtaatcactt tcgtcatctc gtcgggagag atgtcgctgt gccggcgaat
     7321 ttggacggtc aacccggccc ggcgcgcccg ggtcacggcc tggcggaccg tgcgcatgct
     7381 cgggcctgag agccggaact tgtcggggta caggatcgcc tcgtcgccga gctgcagcgc
     7441 gttgagtccg gtcgcccggt acgccgcggc gccgtcgatg ctggcgccca tcacaccggg
     7501 cgcccaaccg tacttctggc acagccgcag ccacgcctcg atggctgcgg gccaagaacg
     7561 cggatcgccg acggggtcgc cgctggccag gcagacgccg acctcgacgc ggtaggtgat
     7621 cgcggcgcga gcgttgtcgg cgaacacgac cgccttgtcg cggcgggtgg cgaagtagcc
     7681 cagcgagtcg ttcttgccgt aaacctcgag cagcccgcgg atcgccgact cgtcctcgcc
     7741 ggtgagcgcg ttctcggcgc gttgcgactg gaaaagcacg accgcggccg ccatcagcgc
     7801 gacggccccg aacaacccga acagcgtgtt gagcagcacg tgcgggtggc cggtaaacag
     7861 gtccggatcg gcgagcgcga aaccgctcac tcggttcagc gcgtagggca gccgttgatc
     7921 gcgaggcagg gtgccgggga atgcctcgac taacccccac gcgagcaggg tgcccaccgc
     7981 cattccgaag accagcacgc cggtggactt caacaacgca ccacggcgaa cccgcgcgta
     8041 gaactccctg tacgagagca gcagcagaac gacggcgccg acatgcaccg ccaagccgac
     8101 gagttctccg aacgtctcga ggcggttctc ggtgtccgac gcgatatcgc cgacgttcca
     8161 acccgccgcg aacacaaggt tgagcaccag cagccaccag gcgatacgtt tgcgggcggc
     8221 cagcgctgcg gcgagcagtg ccagcacgaa cgcccacgca aagctggtgt cggggaagtt
     8281 gaaaatgtag tcgtcgatga attcgcgcgg gaccctgatg atccaccgca tcagcggtga
     8341 cacgctggcc agcaacgaca aagtggcgat gatgccgaca acccacccgg cggtggcggg
     8401 aacccaagag aaccgggaag tcggccgagc ggtcgtgagg gtcatcagcg gcgaggatat
     8461 tcgctcctgg cgtcgaatgg ctccgccgcc taagactctg gggcagtcgg ctctgtcatg
     8521 cctgctagac tggcacgcgc gaccgttcct gagagcatca gggacagcaa gtggagtccc
     8581 gctcccaccg ttggccacac cggcccagcg gtccggtcac ccgccctttg ggcgcggttc
     8641 cgcgcgacgc gcgggcggcg atgagccgcg atcggtcggt atcgggcccc gcatcacgcg
     8701 gggccttttt gctgacgggg tgctgacagc gaggtctcgg cttgctgtct tctcgaactt
     8761 cgctcaagaa cgccgtcgca acgacacaat tatgtacgac ccagggaggc cccatcacca
     8821 ctgagacccg cgtcaacgag cgcatccgag ttccagaagt ccgattgatc ggacccggcg
     8881 gagagcaggt cgggatcgtg cgcatcgaag acgctcttcg cgtcgccgct gacgctgatc
     8941 tcgaccttgt cgaagtcgct cccaacgcca gaccgccggt ctgcaagatc atggactacg
     9001 gcaagttcaa gtacgagaca gctcaaaagg cgcgcgagtc tcgcaagaac cagcagcaga
     9061 ccgtcgtcaa agaacagaag cttcgtccca agatcgacaa ccacgactac gagaccaaga
     9121 agggtcacgt gatccgattt ctcgaagccg ggtccaaggt caaggtgacg atcatgttcc
     9181 gcggccgcga gcagtcccgc ccggaattgg gctaccggct gttgcagcgg ctgggcgccg
     9241 acgtcgccga ctacggcttc gtcgaaacct cggccaagca ggacggccgc aacatgacga
     9301 tggtgctggc gccacaccgc ggtgcgaaga ctcgcgcccg ggcggcgcaa caggccagcg
     9361 gtccgccgaa tggccagcca gactcgaccg acacttcaca gaactgagga catatgccca
     9421 aggccaagac ccacagcggc gcatcgaaac ggtttcggcg caccggaacc ggcaagatct
     9481 tgcgccagcg ggccaaccgc cggcacctgt tagagcacaa gcccgcgaag cgcacacggc
     9541 gactcgaggg ccgcgtcacg gtgtcgacca aagacgcccg ccgaatccga aagttgctga
     9601 acggctgacg ccttcgcacg acctgtttgt acgaacgaga taggaacaac gagccatggc
     9661 acgcgtgaag cgggcagtca atgcccacaa gaagcgccgc accatcctga aagcctcgaa
     9721 gggctaccgc ggccagcggt cgcgcctgta ccgcaaagcc aaagagcagc aactgcattc
     9781 gctcaactac gcctaccgcg acaggcgtgc ccgcaagggc gagttccgca agctgtggat
     9841 ttcgcggatc aatgcggcag ctcgcgccaa cgacatcacc tacaaccggc tcatccaagg
     9901 cctgaaagcg gccggcgtag aggtcgaccg caagaacctg gccgagatcg cggtcagtga
     9961 cccgggggcg ttcaccgcgc tcgtcggtgt cgcgagggct gcgctgccgg aggacgtcaa
    10021 cgcgccgtcc aactcgggaa cggccgcctg agtctggcgc tcaccgagcg ctcggcgcgg
    10081 gtggtcgccg cggcgaagct gcaccgccac gtcgcccgca cccgggccgg acggttcctc
    10141 gccgagggac ccaacctggt cgaggcggcg ctgcgatgtg gcgtggcgcg ggagctgttc
    10201 gtcaccggat cggccgcaac gcggtttgac gcactgctcg ccgacgtgga cgttcccgtc
    10261 cacctcgtga cggagcgggc ggcgaatgcg ctgtcggaca ccgttactcc ggtcggtctg
    10321 gtggcggtgt gcgaaccgcc cacggtgaca ctggcggacg tcgtcgccgc acggccgaaa
    10381 ctcgtcgtcg tcgccgtcgc tgtctcggac cccggaaatg ccggcacgct gatccggctt
    10441 gcggacgcaa tgggcgccgg ggcagtggtc ttgtcgggcc acagtgtcga cccgtacaac
    10501 gccaagtgtc tgcgcgcctc agcgggcagc gtgttcgcgg tcccagtcgt cccggacacg
    10561 gatccggcgg ccgttgtcgg agcccttgcc gaggcgggtc tgcaggtgct cgcgacgacc
    10621 gtggacggtg agacgctgct cgacgacatc gagccgctgc tgtcggcgcc gactgcgtgg
    10681 ctgttcggct cggaggcgca cgggctgccc gaggaggtgg cgacgcttgc agaccggcgg
    10741 gtccggatcc ccctagatgg gggcgccgag agcctcaacg tggcggccgc ggccgcgatc
    10801 tgcctgtatt cgagcgcacg tgcgcagcgg cagggttaag cgcagggcgc cgatccagac
    10861 gcccatcatt ccggtgcgca ggtcgccgaa gtacttccat gtcagcgaat caggcccgag
    10921 cggctcgaac cgtcggcttg gcttcgacgg tgaacgtgta gtggatggcg cgctcacgct
    10981 tcctcctggg tactgcccgt gcactgagga taatgttgac aacacacgtt gtctatgacg
    11041 gtgacggggt gtgttatcgg gcagaaatcg ctttgccgca acgctgtgac cagccggttc
    11101 gcgacgccgc ccgggccggt gaggcacagc cgacattgcc ccgtgtcaga accggcctct
    11161 accctggcga tcgtgaaagt cgagccgtcc ggcgaccgat ccggcggcct cggggaagtc
    11221 gacgagtcac cggatgaccg gccggagcaa tcgccagaac agcgaggagc ggcggcatgg
    11281 ctgacgaaca cgaaccgtaa ccccggggtg atcgccgcga tacgtcgggc gcggaaagcc
    11341 ctcccaggtg atccgcactt cggagatcgg ctgtccgcca gcggtttcgg aggtcccgcg
    11401 gcagctgcgc gggccgcgga ccggctactg ggcgaccgcg acgccgcgtc acgcgagttg
    11461 agtctcggcg ccctgcaggt gtggcaggcg gtgagcgaac gcgtcattcg caagccgacg
    11521 caggatgagg tcacgcttgt gttcaccgac ctggtcggct tctcgcagtg ggcgctgcag
    11581 gcgggcgacg acgccacgct caagctgctg cgacaggtcg cgcaggttgt cgagccgcca
    11641 ttgctggaag tcggcggcca ggtggtcaag cgcatgggcg acggaatcat ggccgtcttt
    11701 cccgacccgg tcaccgccat tgccgccatc tgtgcggcca ccgacgctct gaaaaacgtt
    11761 gatgtcgagg gctatacgcc ggtcatgcgg gtgggcatcc ataccggccg gccgcagcgg
    11821 atcggctcgg actggctcgg ggtcgacgtg aacatcgccg cgcgagtgat ggaatgcgcg
    11881 ggcaagggcg gtgtgctggt ttcgggcgct accctggagc ggctgacccc cgagcaactc
    11941 gacgagctgg gcgcatacgc caagcgcttg cgcagaccca tgtttgccca tcgactgtcc
    12001 ggggtaccac cggacatcac gatgtaccgc ctggaaagtc gcaaggacct gacagcagcc
    12061 gatacgacga aagacagccc atcacaggaa tagtggagtc gatggtcacc ggagcgctgt
    12121 gcactctttt tcgattgcgt gtcacggtca gctatgccgt gatgttggtg gctgtggcca
    12181 cgacattgct gatgctcggt ccaggtgtgc gggaactggt gatcagtcgc atgagcacaa
    12241 acctgcacaa tctggaacac gggcgactcg gcacgttgat cggcagcgcg ttcgtcaccg
    12301 agaacggtcc catctgggta tggctgcccg gcttggtgtg cctgctcgct ctggccgagc
    12361 tgttgtggcg cagcggccgg gtgctggtca cctttgcgat cgggcacgtc ggcgcgacgc
    12421 tgatcgtcac cgccggtctt gctgcggcca tccacgtcgg ctggttgccg atctcggttg
    12481 ctcgggccag cgacgtcggg atcagctacg gagccgtggc ggtgctgggc gcgctgacag
    12541 cggcgattcc atcgcggtgg cggcccgcct ggatcggctg gtggctggcg gtcggcctgt
    12601 tggtggtgtg cgccggtgtc gtgggggccg gcgggggaga gttcaccgat gccggacacg
    12661 tcgtcgcgct gctactgggg attctgctgt cccttcggtt cggcgcagtg acccgatgga
    12721 cgcgcgttcg gctggcgctt ctggtgatcg gtgtcgcatt cggctacctg gtcctggtaa
    12781 acaccggtct gccggtcatc ctcgcaccga tcgtcggcac catcggtgca ttggctgccc
    12841 actggctggc ggggcggtgg cgggctcgtg gcgtcctgcc cgcggccgct gcggccgccg
    12901 ctgtgacgac gtagcgacgc aacagcatcg cctatgatcg ccgggtggct gatcagcccg
    12961 tggacctgtc ggaagaagcg ctgaccaagg ccgtcagcgc ggcccgtcac gcgttcggcc
    13021 tcgccaccga cctcgacggg ttggcgcggg ccaagagcga acaccttggc gaccgttcag
    13081 ccgtcgcgct ggcccgccag gcgctcggag cgctcccgaa ggccgaccgc gccgacgccg
    13141 ggcggcgggt caacgtcgcg cgcacagagg tgcagcacgc ttacgacgag cggctcgcag
    13201 cgttgcgggc cgagcgtaac gccgccgtac tggtggccga acgcgtcgac gtgacgctgc
    13261 cctccacccg gcagccgatc ggtgcgcggc atccgatcac gatcctggcc gagcacgtcg
    13321 ccgacacgtt cgtcgcgatg ggctgggagc tcgccgaggg gcccgagctg gaaaccgagc
    13381 agttcaactt cgatgcgctg aacttcccgc ccgaccatcc ggcccgcagc gagcaggaca
    13441 cgttccaggt tgcgcccgac gggtcgcgcc aagtgctgcg cacccacacg tcaccggtgc
    13501 agatccgtgc gttgctcgaa cgggaactgc cggtgtacat cgtctcgatc gggcgtacct
    13561 tccgcaccga cgaactcgac tccacccaca cgccggtctt ccaccaggtg gaaggcctcg
    13621 ccgtggaccg tggtctgacg atggcgcacc tgcgcggcac gctcgacgcg ttcgcccgcg
    13681 ccgagttcgg gccgcagggc cgaactcgct tccgcccgca cttctttccg ttcaccgagc
    13741 cgtcggctga ggtcgacgtc tggttcgccg gcaagaaggg cggccccggt tgggtcgaat
    13801 ggggcggctg cgggatggtg catccaaacg tgttgcgcgc ctgtggcatt gaccccgaac
    13861 agtactccgg cttcgcattc gggatgggtc tggagcgcac gctgcagttc cgcaacggta
    13921 ttcccgacat gcgcgacatg gtcgagggcg acgtccgttt ctcgctgccg ttcggggtag
    13981 gagcctgatg cgggttccgt acagctggct gcgggaagtc gtgcaggccg gcgcaccggg
    14041 ttgggacgtg tcgccagacg agttggaaca gacgctgatc cggatcggcc acgaagtcga
    14101 agaggtcatc gccgtcgggc ctgtcgccgg accgctgacg gtcgggcggg tgctagaggt
    14161 cgaggagctg accgagttca agaagccgat ccgggcggta aaggtcgacg tcggcgagac
    14221 gcagccgcgt gacatcgtct gcggcgcaac gaacttcgtc gtcggcgatc tggtggcggt
    14281 cgcgctgccc ggcacggtcc tgccgggaga tttcacgatc acgacgcgca agacgtacgg
    14341 ccgcacgtcg gacggaatga tcagctcggc ggccgagctc aacctcggcg tcgaccactc
    14401 cggcatcttg gtgctgcccg acggcgcggc gtcaccaggc gacgacgccg cgccggtcct
    14461 cggcctcggc ctcggcgacg tggtgtttca cctggcgatc accccggacc ggggctactg
    14521 cctgtcggtg cgcggcctgg cgcgggagat cgcctgtgcg tgcgacctcg acttcgtcga
    14581 ccccgccgac gttccggcgc tgcccgccga aggggaggcg tggccgctca cgatcgaacc
    14641 ggggacgggc gtgcgtcggt tcgcgcttcg cccggtcacc ggcatcgatc cggcggcggt
    14701 gtcgccgtgg tggatgcagc ggcggctgct gctgtccggc atgcgcccga tctcgccagc
    14761 ggtggatgtg acgaactacg tgatgctcga actcggacat ccgatgcacg cacatgacct
    14821 caagaggatc accggcggct ttcgggtgcg gtttgcgcgg ccgggagaga ccgtcgtcac
    14881 gctcgacgac atcgaacgtc acctgaatcc cgatgacgtg ctgatcgtcg acgacgtcgg
    14941 gaccgcggcg atcggcggcg tgatgggggc cggcaccacg gagatgggcg acgactccac
    15001 cgacgtgctg ttggaggccg cggtgtggga cccagccgcg gtgtcccgca cgcaacgacg
    15061 cctgcacttg ccgagcgagt ccgcccggcg ctacgagcgt tcagtcgacc cggcgatctc
    15121 ggtggcggcg ctcgaccgcg cctcggtgtt gttggccgaa atcgccagcg ggacaatcga
    15181 accgaagctg accgactggc gcggagaccc ttcccgcgac gactggtcga tgccgccgat
    15241 cgacatgccg gccgacttgc ccgaccgtac cgccggggtg cgctacccag acggagcgac
    15301 ccggcggcgg ctggagcagg tcggcaccgc ggtgaccgcc gatgacggga cgctgaccgt
    15361 gatcccgccg agctggcgac ccgacctgcg gcagccggca gacctcgtcg aggaaatact
    15421 gcggctggcg ggtttggatc tcataccgtc ggtcttgccg ttggcaccgc ccggccgcgg
    15481 gctcaccgcc ggacagaagc gacgccgcgc catcggcaag tcgctggcgc tgtcgggcta
    15541 cgtcgaggtg ctgccgacac cgtttctgcc cgcggccgtg ttcgaccagt ggggtctgcc
    15601 cgccgacgat ccgcgccgga atacgatgaa agtgctcaac cccttggagg ccgaccgccc
    15661 cgaactcgcc accacactgc tacccggtct gctggaagcc ttgagccgca acgtttcccg
    15721 cggcaccgtc gatgtgtcgc tgtacgcgat cgagcaggtg gtgcagccca cggagcgtac
    15781 ccgcgccgtc gaaccgctgc cggtggatcg tcgcccgacc cacgaggaga tcgtcgtcct
    15841 cgacgattcg ctgccgcggc aaccacagca cgtcgcagcg gtgatggccg gtctgcgaga
    15901 gccgcgcggg ccgtggggac ccggccgagc ggtggaagcc gccgacgcgt tcgaggtcgt
    15961 gcggaccgtc gcgcgggcca gtggggtgga gctgacgttg cgcgtcgcgc aacacctgcc
    16021 atggcacccc ggccggtgcg ccgaggtcct tgtcggcgag acggtgatcg ggcatgcggg
    16081 ccagctgcat cccgcggtcg tcgagcgcag cggacttccg aagggcacgt gcgcggtcga
    16141 actggacctc gacgcgattc ctatcaccga ggcgatgccc gcgccgaggg tgtcgccgtt
    16201 cccggcggtg ttccaagatg tcagcctcgt cgtggccgac gacgtcggcg cgcaggccgt
    16261 gatcgacgcg gtgcgcgacg gggccggcga gctgctcgag gacgttcggc tgttcgacgt
    16321 gtacaccggt ccgcagatcg gtgagcgccg caagtcggta acgctggcgc tgcggttccg
    16381 ggctgcggat cgcacactga ctgaagacgc ggcgtcggcg gctcgcgacg ccgcggtgcg
    16441 gcgggcggcc gagctcgtcg gcgccgtgca aagggcttag cccaggctga gtcccgttcc
    16501 gcgagcgggc gtgtctgtcc ctcgacacgc cgctgtaggg ggtacaacag cgcacgctcg
    16561 cgccaaggtg agtggagtat ttgtttgcgc tgatctgcat aaccatgcag aatggctgcc
    16621 atgacgacag tcgcggtagc tggcgcgagc gggtacgccg gtggagagat cctgcggttg
    16681 ctgctcgggc atccggcgta tgccgacggt cggctgacca tcggcgcgct caccggggcg
    16741 acaagcgcgg gcagcacggt cgccgagcat catccgcatc tgctgccgtt ggcctcgcga
    16801 gtgctggagt ccaccgaggc ccgcgtcttg tcgggccatg acgtggtgtt tcttgcgctg
    16861 ccgcacggcc actcggcggc gctggccgaa cagctggacg ccgacacgct gatcgtcgac
    16921 tgcggcgcgg acttccgact gaccgccgcg aacgtatggg agaagtacta cgactcaccc
    16981 cacgccggca cctggcccta cgggctgccc gaacttcccg gcaaccgcga ccgcctgcgc
    17041 ggcgcccgcc gcatcgccgt gccgggctgc tacccgaccg cggcgctgct ggcgctgctg
    17101 ccggccgtgg ccgaaaacct gatcgagccc gtcgtcacgg tcgtggccgt cagcgggacc
    17161 tccggcgctg ggcgcaccgc caagccggaa ctgctcggct ccgaggtcat cgggtcggcg
    17221 cgcgcctaca acatcggcgg cgtccaccgg cacaccccgg agatcgccca gggcctgcag
    17281 gcgctgaccg accgcgcggt gcacgtctcc ttcacgccgg tactcatccc gatgtcccgc
    17341 ggcattctgg ccacctgtac ggcgcggacg gcgaccccgc tgtcgcggct gcgggaggcc
    17401 tatgaaaagg cttacgcatc agagcctttc gtgcatctga tgccggaagg ccagcttccg
    17461 cgcaccggcg cggtgatggg cagcaatgcc gcgcaggtcg cgatcgcggt cgacgacgac
    17521 gcgggcatgt tcgtggcggt gtgtgcgatc gacaacttgg tcaagggcac cgggggtgcc
    17581 gcggtgcagt cgatgaacct cgcgttggga tggcctgagg gcgaaggtct ttcggtcgtt
    17641 ggggtggcac catgaccggg gtatccgccc agacacggct ggtccgcaca cagggtgtca
    17701 ccgcgcccgc aggctttcgc gcagctggaa ttgccgccgg catcaaagcg tcagggaact
    17761 tggacctggc gttggtgttc aacgagggcc cggactacca cgccgcaggg gtgttcaccc
    17821 gcaaccaagt caaggccgcc cctgtgctgt ggacccaaca ggtgatgacg accggtcgtc
    17881 tgcgcgcggt aatcctgaac tccggtggcg caaacgcctg caccggacac caaggctttc
    17941 aggacacgca cgcgaccgca gaagcggtgg ccgaagcgct atctgactgg ggcacggaga
    18001 caggcgcgat cgaggtggcg gtctgctcca ccgggttgat cggtgaccgg ctgccgatgg
    18061 acaaggtgct gtccggggtg cgcgacgtcg ttcacgacct ggccggcgga ctgtccggcg
    18121 gcgaggaggc ggcgcgggca atcatgacga ccgacaccgt cccgaagcag gtcgcgcttc
    18181 accacggtgg caactggacc gttggcggta tggcgaaagg cgcggggatg ctggcgccgt
    18241 cactggccac catgctgtgc gcgatcacca ccgacgccgt cgccgacgcc cgcgcactcg
    18301 acgccgcgct gcgccgcgcc gtcgcgctca cgtttgaccg gctagatgtc gacggcagct
    18361 gctccaccaa cgacacaatc ctgttgatgt cttccggtgc cagtgaaatc acgcccagcc
    18421 aggcggatct cgatgatgcg ctgctgcagg tgtgcgacga tctcgtcgcc cagctgcagg
    18481 ccgacgccga gggtgtcacc aagcgcgtga cgatcaccgt caccggcgcg gccaccgaga
    18541 acgacgcgct caccgtagcg cgggtgattg cccgggacag tcttgtcaag accgcgatgt
    18601 tcgggtccga ccccaactgg ggtcgggtcg tggccgctgt cgggatgggg ccggtgcggc
    18661 tcgatcccga tcgtattacc gtgtcgttca acgacttccc ggtttgtgtc gacggagtcg
    18721 gtgcgccggg tgcgcggacc gtggacctca gcggtcccta catccacatg acggtcgaca
    18781 tcggtgtggg ctccgagcaa gcgtcgatcc gcaccaccga cctgtcccat ggctacgtcg
    18841 aagagaactc ggcatacagc tcatgagcgc tccagctgtc accgcccacg tcaaagccca
    18901 ggtcctcgcc gaggctctgc cctggctgaa agcgctgaac gacaagatcg tcgtcgtcaa
    18961 gtacggcggc aacgcgatga tcgacgacgc gctacggcag gcgttcgccg ccgacatggt
    19021 gttcctgcgc aactgcggca tccaccccgt cgtcgtgcac ggcggtgggc cccaaatcac
    19081 cgcgatgctg aaacgtctcg gtattgcagg cgatttcaag gcgggtttcc gcgtcactac
    19141 gccggaggtg cttgacgtcg cgcggatggt gctgttcggc caggttggcc gagaactcgt
    19201 gaatcagatc aacgcgcacg gcccgtatgc tgtcgggatc accggcgagg acgcccattt
    19261 gttcaccgcg gtgcgacgcc gcgtcaccgt cgacggcgtg gacaccgaca tcggcctcgt
    19321 cggtgacgtc gcaaccgtca acaccgccgc agtactggat ctgattgcag ccgggcgcat
    19381 tccggtggtg tcgtcgatcg cacccgacgc cgacggcatc gtgcacaaca tcaacgccga
    19441 caccgccgcg gctgcgctcg ccgaagcgct ggaggccgag aagctgctga tgctcaccga
    19501 cgtcgaaggt ctctacgccc gctggcccga ccgcgactcg ttcgtcagcg aaatcgacac
    19561 ggagacactg tctgagctgc tgccgtcgct ggatgccggc atggtgccga aggtagaggc
    19621 ctgcctgcgg gcggtggccg ccggggtgcc cagcgcgcac gtgatcgacg gccgcgtcga
    19681 tcactgtgtg ctggtcgaac tgctcacgga cgccggcacc gggacgaagg tggtgagggc
    19741 atgacactcg aagagcgctg ggcggcggtg atgatggaca actacggcac accgccgctg
    19801 gccctggcaa gcggcgacgg ggccgttgtc accgatgtcg acggcaaccg ctacctcgac
    19861 ctgctcggcg ggatcgcggt caacgtgctc ggccaccgtc atccagcggt catcgacgcg
    19921 gtgacccgcc aactgaacac gctcggccac acgtcaaacc tctacctgca cgagcccgga
    19981 attgcgttgg ccgaggtgct ggttgggctg ctcggcgccg acgccgaaac ccgtgtgttc
    20041 ttctgcaact cgggtgcgga ggcaaacgag gtcgcgttca aactgtcgcg tctgaccggg
    20101 cggacgaaac tcgttgcggc gcaggaggct ttccacggac gcaccatggg agccctggcg
    20161 ctcaccggcc agcccagcaa gcaggcgccg tttgcgccgt tgccgggtga ggtgacgcac
    20221 gtgccctacg gcgacgccga cgcgctggcc caagccgtgt cggacgacac cgccgcggtg
    20281 ttcctcgagc cgatcatggg ggagagcggc gtcgtcgtcc cgcccgcggg ctatctcgct
    20341 gcggcgcggg agatcaccgc gcgtcacggg gctctgctgg tgctggacga ggtgcagacg
    20401 gggatgggtc gcaccggtgt gttcttcgcg caccagcacg acggcgtcac ccctgacgtc
    20461 gtgacgatgg ccaagggact cggcggcggc ctgccgatcg gggcgtgcct ggcggtcggt
    20521 ccggccgcac ggctgatgac tcccggcctg cacggcagca cgtttggcgg caacccggtg
    20581 tgcgcggcgg ccgcgctcgc cgtgctgcgg acgatcaccg aacaggacct gatcggccgc
    20641 gccggcgtgc tgggcaagac gttgagccac ggtatcgagt caatgaatca tcccttggta
    20701 gaccatgtcc ggggtcgcgg cctactgcgc ggtgtggtcc tgacgtcgcc ggtggccaag
    20761 cccgtcgagg ccgccg
//
LOCUS       DASWBF010000596         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_656696,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000596 DASWBF010000000
VERSION     DASWBF010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656696"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VF874_22155"
     CDS             complement(<1..410)
                     /locus_tag="VF874_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020172348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX9178667.1"
                     /translation="MTNPSAAYGVHSEVGKLRKVLVCSPGLAHERLTPTNCDDLLFDD
                     VLWVQSARRDHFDFMAKMRDRGVEVVELHNLLTETIQIPEAKQWLLDRKIVANEVGPG
                     LVADTRAFLDSLEPRRLAELLIGGMSTLDLPADMR"
     gene            488..874
                     /locus_tag="VF874_22160"
     CDS             488..874
                     /locus_tag="VF874_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX9178668.1"
                     /translation="MVRLLLRGVVFLASSAIGLLVAAWLIPGVSVTVSGFVVAVVVFS
                     VAQAILSPFIFKMARRYASAFLGGIGLLSTLVALILASVLTHGVSIRGLGSWVAATVV
                     VWLVTAAATLMLPLLVIREKRSGQPQ"
     gene            complement(868..1974)
                     /locus_tag="VF874_22165"
     CDS             complement(868..1974)
                     /locus_tag="VF874_22165"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HEX9178669.1"
                     /translation="MKTKAAVLWGINQKWEVEEVDLDGPKEDEVLVKMTASGLCHSDY
                     HLVTGDLPAPLPLVGGHEGAGVVVDVGPGVKEVAEGDHVVMSFLPACGRCKPCARGMS
                     NLCDLGAYLLTGAQLDGTFRFHARGQDVGQFLLLGTFSEYAVLPTASVIKLDDAHPLD
                     KAALLGCGVTTGYGSTVRTGDVQAGDTVVVIGVGGVGINAVQGARIAGARTIVAVDPV
                     EYKRERSREFGATHVAASVDEALPLVKDLTWGEMANVCVLTTNVAEGEYVGPALSLVG
                     KRGRAVITAIGRATDTTVSMSLFELTMWEKQVRGGLFGSSNPRSDIFELLSLYESGQL
                     KLDELITREYTLEDVNQGYDDMLSGQNLRGLIRY"
     gene            2048..3673
                     /locus_tag="VF874_22170"
                     /pseudo
     CDS             2048..3673
                     /locus_tag="VF874_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729766.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
     gene            3661..>3840
                     /locus_tag="VF874_22175"
     CDS             3661..>3840
                     /locus_tag="VF874_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178670.1"
                     /translation="MSPLIGESRVLYSTMQDIPLTLTSILRHGTSIHGERVVITATED
                     GYRQIDYREFGARVAQ"
BASE COUNT          618 a         1264 c         1278 g          680 t
ORIGIN      
        1 cgcatgtccg cgggcaggtc gagtgtcgac ataccaccga tcagcagctc ggccagccgc
       61 cgcggctcca gcgaatccag gaacgcccgg gtgtcggcga ccagccccgg cccgacctcg
      121 ttggcgacga tcttgcggtc cagcagccac tgcttggcct cggggatctg gatggtctcg
      181 gtcaataagt tgtgcagctc gacgacctcg acgccgcggt cgcgcatctt ggccatgaag
      241 tcgaagtgat cgcgacgggc gctctgcacc cacaacacgt cgtcgaacaa caggtcgtcg
      301 cagttggtcg gcgtcagcct ctcgtgggcc agtccgggag agcagaccag caccttgcgc
      361 agcttgccca cctcggagtg cacgccgtag gcggcagagg gattggtcat ggtgtggtcc
      421 tttccgggtg ctggcgcgca tcaccaaaat gggttggtta gtcgtcattg gccttacgat
      481 cgtcgccatg gtgcgacttc tgctccgcgg cgtggtgttt ctcgcgtcgt cggcgatcgg
      541 attgctggtg gccgcgtggc tgattccggg tgtctcggtc acggtgtcgg ggtttgtcgt
      601 tgctgtcgta gtgttctccg tcgcgcaggc catcctgtcg ccgttcatct tcaagatggc
      661 caggcgatac gcgtcggcgt tcttgggtgg catcgggttg ctgtcgacgc tggtcgcgct
      721 gatcctcgcc tcggtgctca cccacggcgt gagcatccgc ggactcggat catgggttgc
      781 agcaacggtg gtggtgtggc tggtgactgc ggccgccacg ctgatgctgc cgttgctggt
      841 catccgtgag aagcgctcgg gccagcctca gtagcgaatc agcccgcgca ggttctgccc
      901 gctgagcatg tcgtcgtaac cctggttcac gtcttcgagc gtgtactcac gggtgatcag
      961 ctcgtcgagc ttgagctggc cgctctcgta cagcgacagc agctcgaaga tgtcgctgcg
     1021 cgggttcgac gagccgaaaa ggccaccgcg gacctgtttt tcccacatgg tcagctcgaa
     1081 cagcgacatg ctgaccgtcg tgtcggtcgc gcgcccgatc gcagtgatca cggcgcggcc
     1141 ccgcttgccg accaggctca gcgccggccc gacgtactcg ccttcggcca cattggtggt
     1201 cagcacgcac acgttcgcca tctcgcccca cgtgaggtcc ttgaccagcg ggagcgcttc
     1261 gtcgaccgac gcggcgacat gtgtcgcgcc gaattcccgt gaccgttcgc gcttgtactc
     1321 gaccgggtcg acagccacga tcgtgcgcgc gccggcgatc ctggccccct gcaccgcgtt
     1381 gattccgaca ccgccgacgc cgatcacgac caccgtgtca cccgcctgca catctccggt
     1441 gcgcaccgtg ctgccgtatc cggtcgtcac gccgcagccc agcagcgccg ccttgtccag
     1501 cggatgggcg tcgtcgagct tgatcaccga ggctgtcggc agcacggcgt actccgagaa
     1561 cgtgcccagc agcaggaact ggccgacatc ctgaccgcgg gcgtggaacc ggaacgtgcc
     1621 gtcgagctgg gctccggtca gcaggtatgc gccgagatca cacaggttgc tcatgccgcg
     1681 ggcacagggc ttacagcggc cgcaggccgg caggaagctc atgaccacgt ggtctccctc
     1741 ggcgacctct ttgacccccg gcccgacgtc caccaccaca ccggcgccct cgtgcccacc
     1801 cacgagcggc agcggtgcgg gaaggtcgcc agtgaccagg tgatagtcgg agtggcacag
     1861 tccgctggcg gtcatcttga ccaggacctc gtcctccttg ggcccgtcga ggtcgacctc
     1921 ctcaacctcc cacttctggt tgattcccca cagcaccgcc gccttggtct tcatccctgt
     1981 gcccttcttg tcagtcgtgc gtcgattatt ggccttcggc ggctgccgcc gggccaagat
     2041 ggaaagcatg gacagcacca tgcaggactt cccgctgacg atcaccgggg tcctccgtca
     2101 cgggaccagt tggcacgcgg accgcaaggt gattaccgcg acggacgacg gctaccggga
     2161 ggccagctac ggcaccgtcg gtgagcgtgt cgcgcagctg gcgcacggcc tgcgtgggct
     2221 gggaatcacc ggtgacgagc gggtggccac gttcatgtgg aacaaccagg aacacctgga
     2281 ggcctacttg gccgtgccgt gcatgggcgc cgtgctgcac acgctgaaca tccgcctgcc
     2341 cgccgaccaa ctcgcctaca tcgccgaaca ggccgaggac cgggtcgtgg tggcggatat
     2401 gtcgctcgcc ccgctgctgg ccgctgtgct gccccagatg ccgacggtgc acaccgtggt
     2461 cgccgtcggt gacggtgatc tcgacgcgtt cgccgagtcg ggcaagaccg tcgttcgcta
     2521 cgacgacctg ctggccggcc agccgacggt gtacgactgg cccgccccgg acgaaaagtc
     2581 tgctgcggca atgtgttaca ccagcggcac caccgggaac ccgaagggcg tggtgtacag
     2641 ccaccgctcg gcgtatctgc agtcgatggc gatctgcgcg gccttcgcgc tctccgaaga
     2701 cgaccgcata ctgccgatcg tgccgatgtt ccatgccaat gcctggggca atccgtacgc
     2761 ggcgctaatg gccggggccg acctgctgat gcccgaccgg ttcctgcagg cggagcgcct
     2821 ggtggagatg atcgagacgc accgccccac cgtctctgct gcggtaccga cgatctggaa
     2881 cgacgtgctg acgtacctga gctctcaccc ggagcgggac atctcgtcgc tgcgggtggt
     2941 gccgtgcggc ggttcggccg tgccgctggc gatgatgaga gagttccagg acaagcacgg
     3001 cgtcgagatc cgccaggcgt ggggcatgac cgagacgtcg ccgctggcgt cgatcgcccg
     3061 gccgcccggc gacgtcagcg acgaggagca ttgggcgctg cgcaataccg caggccgccc
     3121 gatgtgcgga gtcgaggcgc gcatcgtcga cgacgacggc aacgtgcttc cgaatgacgg
     3181 aaaagcggtg ggggaggtgg aagttcgcgg tccctgggtg accgggtctt attacctcaa
     3241 cgccgacgag tcgaagttcc acgatgggtg gctgcgcacc ggcgacgtcg gccgcatgga
     3301 cgcgcgcggc ttcatcacgc tgacggatcg cgcgaaggat gtgatcaagt ccgggggcga
     3361 gtggatctcg tcggtggagc tggagaacca cctgatggcg caccccgacg tgttcgaggc
     3421 cgccgtcgtc gcggtgcccg acgaccgttg gcaggaacgg ccgctggccg cgatcgtcgt
     3481 ccgggaggac gccaacgtca ctgcccagga gttgcggaac ttcctgtgcg acaaggtggc
     3541 tcgatggtag ctgccggagc ggtggacctt cgtcgacgaa gtgcccaaga ccagcgtggg
     3601 caaattcgac aagaagctga ttcggtcacg ctacgccgat caggcgtacg acgtggtcga
     3661 gtgtcaccac tgatcggaga aagccgcgtg ttgtacagca cgatgcaaga cattccgctg
     3721 acgctgacgt cgatcctgcg tcacggcaca agcatccacg gcgagcgggt cgtgatcacc
     3781 gccaccgaag acggataccg tcagatcgac taccgggagt tcggtgcccg cgtcgcccag
//
LOCUS       DASWBF010000597         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_986169,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000597 DASWBF010000000
VERSION     DASWBF010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986169"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VF874_22180"
     CDS             complement(<1..629)
                     /locus_tag="VF874_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9178671.1"
                     /translation="MTRAHVQGTPFGDLLRDWRQRRRMSQLDLAFAAAVSSRHVSFME
                     TGRSTPSRAMVLHLAAALDVPAREQNHLLVAAGLAPVYAERSLDDPDMAAVRAGVEQV
                     LAAYDPFPCLAVDRGWRVLRFNAGATLLLDGVAPHLLDEPNALRIALHPDGLAPRIRN
                     LAEWRHHVAGRLRREIAAGGPAQLLSLLDEIGSYPGGFATTRNLGGVAVP"
     gene            716..1105
                     /locus_tag="VF874_22185"
     CDS             716..1105
                     /locus_tag="VF874_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178672.1"
                     /translation="MRNDPASAATIPRWLRFVLISDRAGSSWYVGTGFFFAPVLAVVS
                     PWPTVTAILWVLIGLAGLWLGLLGIAMAMGLAMVLRAGVEIPEEYWWSILDDSRAASL
                     PPEPQPRRIAARGIRAPALCGPLCRRQ"
     gene            1324..1650
                     /locus_tag="VF874_22190"
     CDS             1324..1650
                     /locus_tag="VF874_22190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178673.1"
                     /translation="MELMNPRSSVAYGEFDWVTVELDGGWAAAEGSDSVVHDFLATSL
                     TNAKVATTAPIPANTESVDITRDRKPATESVLHAIECCGQETAIASWIPRAECAFDHE
                     FDTTWR"
     gene            complement(1663..1995)
                     /locus_tag="VF874_22195"
     CDS             complement(1663..1995)
                     /locus_tag="VF874_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178674.1"
                     /translation="MTYRTRTWKAAAAILLGFAAIPGAVVIVQPPAVATADVCASVGR
                     RVSVSGCANIADTIADYVPPPSYYAPMPDDEPAPEYTTPPPPPPPPAPNVSVCANVGR
                     RVNVSGCV"
     gene            complement(2134..3264)
                     /locus_tag="VF874_22200"
     CDS             complement(2134..3264)
                     /locus_tag="VF874_22200"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX9178675.1"
                     /translation="MHTAICDELGIEFPIFAFTHCRDVVVAVSKAGGFSVLGAVGFTP
                     EQLEIELNWIDEHIGDHSYGVDIVIPNKYEGMDSNLSAEELTKTLQSMVPQEHLDFAR
                     KILADHGVSVEDSEQDALQLLGWTEATATPQVEIALRHPKMTLIANALGTPPADMIKH
                     IHDEGRKVAALCGSPSQARKHADAGVDIIIAQGGEAGGHCGEVGSIVLWPQVVKEVAP
                     VPVLAAGGIASGQQIAAALALGAQGAWTGSQWLMVEESENTPVQQAAYAKASSRDTVR
                     SRSFTGKPARMLRNDWTEAWETPGNPEPLGMPLQYMVSGMAVKATHKYPSETVDVAFN
                     PVGQVVGQFTKVEKTSAVIERWVQEYLEATNTLNELNEAASV"
BASE COUNT          560 a         1114 c         1147 g          655 t
ORIGIN      
        1 ggcaccgcga ccccgccgag attccgagtt gtcgcgaaac cgcccggata cgacccgatc
       61 tcgtccagca gcgacaacag ctgtgccggg ccgccggcgg cgatctcacg acgaagcctg
      121 cccgccacgt ggtggcgcca ctccgccaga ttccggatcc gaggcgccag gccgtcgggg
      181 tgcagcgcga tccgcagcgc gttcggttcg tcgaggaggt gcggtgcgac accgtcgagc
      241 agcagcgtgg cgccggcgtt gaaccgcagg acgcgccatc cgcggtcgac ggccaggcac
      301 ggaaacgggt cgtaggccgc gagtacctgc tcgacgccgg ctctcacggc cgccatgtcg
      361 ggatcgtcga gggagcgctc ggcgtagacg ggcgccagcc cggccgcaac caacagatgg
      421 ttttgttcac gtgccggcac gtccagcgct gcagcaaggt gcaacaccat cgcgcggctg
      481 ggcgtcgagc gtcccgtctc catgaaactg acgtgccgcg acgacacggc agcggcgaag
      541 gcgaggtcga gttggctcat ccggcgccgc tgccgccagt cccgcagcag atcaccaaat
      601 ggcgtcccct ggacgtgagc ccgagtcaac cgcctcagtg tggctcggtc gcgttcgcgg
      661 caccactacc ttccaggtaa ttgcatcgcc tacctgatga cggcaggctg aactcatgag
      721 gaacgatccg gcatccgcag ccacgattcc gcggtggctg cgcttcgtgc tgatctcgga
      781 tcgtgccggg tcgtcgtggt acgtcggcac cgggttcttc tttgcgccgg tgctcgctgt
      841 ggtgtcgccg tggccgacgg tcaccgcgat cctgtgggtg ctcatcggtc tggccggact
      901 gtggctcgga cttctcggta tcgcgatggc gatgggtttg gcgatggtgt tgcgggcggg
      961 cgtcgagata ccggaggagt actggtggtc gattttggac gactcgcgtg ccgctagcct
     1021 cccgccggag cctcagccga gaaggattgc ggctcgcggt attcgagcac cggctctttg
     1081 cggaccactg tgtcgccggc agtgatcacg agctgcttgg tggacaacgc gtacgtgaca
     1141 ccgtcgttgc gtgcgacgaa ttctcgggca gcggtgtttt cggctggaac attcagcacc
     1201 gctccgtcgc ggagccgcac gccgcggtac tggtaaccgc ccttcgagtc ggtgcagatc
     1261 gccaccagag atccctgcgt gcggacgaag gcgactcccg tcagcggacc ttcgcatcgc
     1321 gcggtggagt tgatgaatcc gcgatcatcg gtcgcgtatg gcgaattcga ttgggtgaca
     1381 gttgaactgg atggcggctg ggcagcggcc gagggcagcg attctgtggt ccatgacttc
     1441 ttggcgacgt cgttgacgaa cgccaaggtg gcaacgactg caccgattcc ggccaacact
     1501 gagagcgtcg acatcacccg agatcgcaag ccagccactg agtctgtcct ccacgcaatt
     1561 gagtgttgcg gccaggaaac cgctatcgct tcctggatac ctcgcgcgga gtgcgcgttc
     1621 gatcacgaat tcgacacaac ttggcgctga ttgcgccgta cgtcagacgc agccgctgac
     1681 gttgacccgt cgtcccacat tcgcgcacac gctgacgttc ggcgccggcg gtggcggcgg
     1741 cgggggaggc gtggtgtact caggcgccgg ttcgtcgtcg ggcatggggg cgtagtagga
     1801 cggtggcgga acatagtcgg cgatcgtgtc ggcgatgttg gcgcagccgc tcaccgacac
     1861 ccgacgaccg acactcgcgc agacgtccgc ggtggcaacc gccgggggct ggacgatcac
     1921 gactgctccg ggaatcgcgg cgaatcccag aaggattgcc gccgcggcct tccatgtacg
     1981 ggtccgatac gtcatcacac tgtctcctca accctgcgcc gtcgccggat gggcaagtat
     2041 atagagatgt gcccgcttgc cgcgttggga taacgaaaac tgttgtggcg ctagtctttc
     2101 ggtactcagc atgcaccaaa cgctaaacgg gcgtcagacg gaggccgcct cgttgagctc
     2161 gttgagcgtg ttcgtcgcct ccaggtactc ctgtacccac cgttcgatga cggctgaggt
     2221 tttttcgacc ttggtgaact ggcctacgac ctgacctacg gggttgaacg cgacgtcgac
     2281 ggtctcgctc gggtacttat gcgtggcctt aacggccatg ccggacacca tgtactgcaa
     2341 cggcatgccc agcggctcgg gattgccggg cgtctcccag gcctcggtcc agtcgttgcg
     2401 cagcatccgg gccggcttgc cggtaaacga ccggctgcgg accgtgtcgc ggctgctggc
     2461 ctttgcgtat gcggcttgct ggacgggcgt gttctcggac tcctcgacca tcagccactg
     2521 cgacccagtc cacgcgccct gcgcaccgag cgccaaggcc gccgcgatct gctggccgct
     2581 ggcgatgccg ccagccgcca gcaccggaac cggcgcgacc tccttgacca cctgcggcca
     2641 cagcacgatc gagccgacct caccacagtg cccgcccgct tccccgccct gggcgatgat
     2701 gatgtcgacg ccggcgtcag cgtgcttacg tgcttgcgat ggcgagccac acagcgcggc
     2761 gaccttgcga ccttcgtcgt gaatgtgctt gatcatgtcg gccgggggag tgccgagcgc
     2821 gttggcgatc agtgtcatct tcgggtgccg caacgcgatc tcgacctgtg gagtcgcggt
     2881 cgcttcggtc cacccaagca gttgcaaggc gtcctgttcg ctgtcctcga cagaaacgcc
     2941 gtggtcggcg aggatcttgc gggcgaaatc gaggtgctcc tgcggcacca tcgactgcag
     3001 cgtcttggtg agttcctcgg cggacaggtt cgagtccatg ccctcgtact tgttcgggat
     3061 cacgatgtcg acgccgtaag agtggtcgcc gatgtgctcg tcgatccagt tgagttcgat
     3121 ctcgagctgc tccggtgtga agccgaccgc gccgagcacg ctgaagccgc cggccttgct
     3181 caccgctacg accacgtcgc ggcagtgcgt gaatgcgaag atcggaaact cgatgccgag
     3241 ttcgtcgcag atggcggtgt gcattacctg ctcctttgga cgcgggcggc gaactgaaac
     3301 gtgttctagt ttaataccaa gcagcggcgg gggcaccggc ggggtctctg cccgcccttc
     3361 ccttcccgcg aaaccgacgt ttcgcacgtt tgcgcacttt ctgtgcgaaa cgctggtttc
     3421 gcaggcagcg gtagcccaca taacctccgc gcgtctggtt catatcggtt cggtaa
//
LOCUS       DASWBF010000598         3807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_581918,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000598 DASWBF010000000
VERSION     DASWBF010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581918"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1286
                     /locus_tag="VF874_22205"
                     /pseudo
     CDS             282..1286
                     /locus_tag="VF874_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1361..2788)
                     /locus_tag="VF874_22210"
     CDS             complement(1361..2788)
                     /locus_tag="VF874_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX9178676.1"
                     /translation="MSAGEIRCLVTGATGYVGSRLMPRLLDRGYRVRAMARNPDKLAD
                     VPWRDRAELVWGDLGDLDSLVAAFDGVDVVYYLVHSMGTSKDFVADEGRAAHNVVTAA
                     RRAGVRRLVYLSGLHPPGTQLSPHLESRKAVGDILLGSGIETIVLQAGVVVGSGSASF
                     EMIRHLTDRLPVMTTPKWVHNKIQPIAVGDILHYLVAAATAEVPSSRTWDIGGPDVLE
                     YGDMMRIYADVAGLGKRYLIVLPFLTPTIASLWVGTVTPIPPGLARPLIESLECDAVM
                     RDHDIDTAIPPPPGGLTRYRRAVALALNRAARGLVDATWSDAYPDGAPAEALPSDPDW
                     AGEIVYTDVRTLLTPVDQAELWKVVQKITETTGGGSRWYSYPLSTMARGRAQRWTVED
                     HESGNLLRLHAERRAPGEAWLEMRVTPGPGAGSRYEQRAIFLPRGVTGRLYWYVFRLW
                     HVLALRAMARNVVESATASRFDLKK"
     gene            complement(2978..3637)
                     /locus_tag="VF874_22215"
     CDS             complement(2978..3637)
                     /locus_tag="VF874_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX9178677.1"
                     /translation="MMARIAALVWQLVEAACSVVGLRYGTEEPHYTVQRRTHGLEIRR
                     YGPRVAAETTITADEDAARNAGFRRLAGYIFGGNHRRAKIAMTAPVAQQSTEAEGGQN
                     VAMTAPVSQAVGPGGSSVIRFFMPSKWTQDRLPEPNDETIRLVNVPAETVAVLRFTGS
                     RSPSVVASRKSELLQLLRDTDFHPSGTPVAWFYDPPWTIPFRRRNEIAVPVMPVGEPV
                     P"
BASE COUNT          650 a         1281 c         1255 g          621 t
ORIGIN      
        1 cccaagctcg tttgcgttgc cgctgacacg gtcgatctgc gattgaactt catcacaccg
       61 ctcctggtgg cgggagctgt cgcggcagcg atcaccgcag ctgatgacag caagagtttt
      121 acgcatttat gttttgcctc caagcgtttt gatcatggat gaccgggaat gggccgaacc
      181 agtgtcgtct agtctgtcga tacgacggca agcaatcagc ccctcaacgt cgtggtttat
      241 gtggttagtt agcagaacgt ccacacgacc tcaagttgac agtgccggcc aatgtggcct
      301 atccgaccga ttcgggtctg ctggcccgcg cgattcgccg gatcgccgcg accggtaaac
      361 ggatccaagc cgcaggcggg gcgacgcgca ccaagctgcg ggatcgctcg cgggcggccg
      421 ggcggcgcgc gcatgagatc gccgccaagc tgcgcacccg ctcggtggcc gggcgcggtg
      481 aggtgccggc cacggtgcgg cgggtcaccg gggagctggc cgagctggcc gccaccaccg
      541 ccgccgacgc tcaacggctg ctcgtcaacg ccaaacgggc gctgcgccgg gcccgggccg
      601 aggccgatga gctgcgcaaa tgtggggtgc gcgatgcggc ggcggcgggg cggcggcgcg
      661 ggcggctggc gcgtgcggtc aacgagctga ccgggctgct ggatgcgacg cggcagatcg
      721 tggcacagac tcggcaacgc gtcgctggga tcacccccga tggggcgacg cggcgggtca
      781 gcctgcacga cggcgatgcc cgcccgatcg ccaaggggcg gctgggtcgg ccggtggagt
      841 tcgggcacaa ggcccaaatg gtggacaacg atgacggcgt cgtcccggat cacgctgtgg
      901 agcagggcaa tcagcccgac gcgccgcaac tggctcccgc ggtggaacga gtgaagaagc
      961 gaaccgggcg cacgccgcgc acggtcaccg ccgaccgcgg ctacggcgaa gccagcgtcg
     1021 ataagcagct caccgacctc ggtgtcacca acgtggtcat cccgcgaaag ggcaaaccct
     1081 ccgaagcgcg gcgagccgaa gagcatcgaa aagcgttccg ccgcactgtg aaatggcgca
     1141 ccggggccga aggccgcatc agctgtctca aacgcggata tggctgggac cgcacctgca
     1201 tcgacggcct cgaaggcacc cggatctgga ccggccacgg cgtcctcgcc cacaacctgg
     1261 tcaagatcag cgccctcgcc gcatgacagc ccacccgaag cccgccgcag tcccgggcca
     1321 gcatcatcgc cagcggcaac gcgtgtccga tccagcaccg tcactttttc aggtcgaagc
     1381 ggctagctgt tgcgctctcg acgacgttgc gggccatcgc ccgtaatgcc agcacgtgcc
     1441 acagtcggaa gacgtaccag tacagtcggc ccgtaacgcc gcggggcaag aagatcgccc
     1501 gctgttcgta tcggctgcct gcgccgggtc ccggcgtcac ccgcatctcc agccacgcct
     1561 ccccaggggc ccgcctctcg gcgtgcagcc gcagtaggtt gccggattcg tgatcctcca
     1621 cagtccagcg ctgggcccgc ccgcgcgcca tcgtggacag cgggtaggag taccagcggc
     1681 tcccgccgcc ggtggtttcg gtaattttct ggacaacctt ccagagttcg gcttggtcca
     1741 ccggggtcaa cagggttcgc acgtcggtgt agacgatctc gccggcccag tcggggtcgc
     1801 tcggcaacgc ttcagcgggc gcaccgtccg ggtaagcgtc ggaccaagtg gcatcgacga
     1861 gtccccgggc cgcgcggttc agcgccagcg ccaccgcgcg ccggtaccgg gtcagcccgc
     1921 ccgggggtgg cgggatcgcg gtgtcgatgt cgtggtcgcg catcaccgcg tcgcactcca
     1981 gcgactcgat cagcggccgg gccagcccgg gcggaatcgg ggtcacggtg ccgacccaca
     2041 ggctggcgat cgtcggcgtc aggaacggca gcacgatcag gtaccgcttg cccagtccgg
     2101 cgacgtcggc gtagatccgc atcatgtcgc cgtactccag aacgtcgggg ccaccgatgt
     2161 cccaggtgcg tgacgacggc acctcggccg tcgcggcggc gacgaggtag tgcaggatgt
     2221 cgccgacggc gatcggctgg atcttgttgt gcacccactt cggcgtcgtc atgacgggca
     2281 ggcggtcggt gagatgccgg atcatttcga acgacgccga gcccgacccg acgaccaccc
     2341 cggcctgcag cacgatggtc tcgatgccgg atcccagcag aatgtcgccc actgctttcc
     2401 gggattccag atgcggcgat agctgggtgc ccggcggatg caacccgctc aggtatacca
     2461 accgccgcac gccggcgcgg cgggcggcgg tgacgacgtt gtgtgccgcg cggccctcgt
     2521 cggccacgaa gtccttcgac gtgcccatcg aatgcaccag gtaatagacg acgtcgaccc
     2581 cgtcgaacgc ggcgaccagc gaatcgagat cgcccaggtc gccccatacc agctcggccc
     2641 tgtcccgcca cggtacgtcg gccagcttgt cagggttgcg ggccatcgcc cgcacccgat
     2701 aaccgcggtc tagcagccgc ggcatcagcc tgctgccgac gtagcccgtc gccccggtca
     2761 ccaagcagcg aatctcacca gccgacacta agcctccgtt ttgtagacgg tcgcgtccgc
     2821 tggctaaact agtctcctgc cgcagcaact cgacaaggca cggtcaattt gtctgaccaa
     2881 tcgcaccgaa gtgcgctcag cgtcagaccc atcggttgat cagttttagc ctaacgacca
     2941 tcgtcgcggg tggcattgct gtgaccgatc ggggttctta cggcaccggc tcaccaaccg
     3001 gcatcaccgg cacggcgatt tcgttgcggc gccggaaggg gatcgtccag ggcgggtcgt
     3061 agaaccatgc cacgggcgtg ccgctgggat ggaaatcggt gtcgcgcaat agctgtaaca
     3121 gttcactttt acgggacgcg accacgctgg ggctgcggct gccagtgaag cgaagcaccg
     3181 cgacggtctc ggcgggaacg ttgaccagcc tgatcgtctc gtcgttgggt tcaggcagcc
     3241 tgtcctgtgt ccatttagat ggcatgaaga accgaattac cgatgagccg ccgggtccaa
     3301 ccgcctgcga taccggtgct gtcatcgcga cattctggcc accctccgcc tcggttgact
     3361 gttgagcgac cggcgctgtc attgcaatct tggctctgcg gtggttgcca ccgaagatat
     3421 agccggcgag acgccgaaaa ccggcattgc gtgccgcgtc ctcatcggca gtgatcgtcg
     3481 tctcggctgc gacgcgtggg ccgtagcggc ggatctccag accatgggtt cgccgctgca
     3541 cggtgtagtg cggctcctcg gttccgtatc gcagccccac caccgagcag gccgcctcca
     3601 ccaattgcca caccagcgcc gcgattcgtg ccatcatcgc cgcctttctg ttgtccgaca
     3661 aggtaagccg cgacgggttg ctggacagct taggtggtcg gcggcatccg cctcggtccg
     3721 cgcttcttca ggagcttcgt cgtgctggaa ctaaccgagc gaaagccaga acctccggcg
     3781 agaaacacgg cgttgttgtt cgtgcat
//
LOCUS       DASWBF010000599         5743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_986475,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000599 DASWBF010000000
VERSION     DASWBF010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986475"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /locus_tag="VF874_22220"
     CDS             complement(<1..1600)
                     /locus_tag="VF874_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX9178678.1"
                     /translation="MTSEDVPGVEHDLTPLPDLLHGRPRAGPVRDRRPVYVDLLPPCN
                     NACPAGENIQGWLGHARVGKHEEAWRLLVADNPFAAIHGRVCYHPCESNCNRANLDSA
                     VSIHAVERFLGDAARENNWQFAPPPEPSGKKVLIVGAGPSGLSAAYHLARRGHAVEIR
                     DAGAVPGGMMRYGIPNYRLPRDVLDDEVRRIAALGVQITCGHRVEDLADELQTGQFDA
                     AFVAVGAHLAKRVNIPVDDASTMVDALAFLRSVASGEHPEIGPRVAVYGGGNTAMDAA
                     RVARRLGADDTVIIYRRTRAQMPAHEEEAEEAEREGVQIHWLRTIAAFDRHEMQVEVM
                     ELDDNGKPHPTGRYETLAADTVIMALGQETESAFMRNLPGVVFDDDGSVQVSETLMTG
                     YPGVFAGGDMVPCERTVTVGVGHGKKAAHHIDAWLRDTSDERPPKHPIATFDELHLWY
                     FGDAARRAQPELPPDERVAGFEEVVGGLNATAATYEARRCLSCGNCFECDGCLGACPE
                     DAVIKLGQKGDRYRFDYDRCTGCGE"
     gene            complement(1630..2865)
                     /locus_tag="VF874_22225"
     CDS             complement(1630..2865)
                     /locus_tag="VF874_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9178679.1"
                     /translation="MSVDSIGTARRWSMLAIALSSTMFANVFINGAAFLIPTLHERRG
                     LDLADAGLLSAMPSIGMVFTLIAWGYAVDRLGERLVLALGSALTAAAAFAAGAAGAAT
                     SGPAASNSIMLTGTFLLLGGMAAASSNSASGRLVVGWFPAERRGLAMGIRQTAQPLGV
                     GLGALVIPQLAQNHGIGTALLFPAVVCAVSAIVSAVGVVDPPRPPREQAPQEDLANPY
                     RGSSVLWRIHAVSVLLVVPQVVVWTFTLVWLMTDRGWSARSAGALVTTAQVLGALGRV
                     AAGRWSDRVGSRLRPIRTIAAAAALTMGLLAVTDYWHSPLAVAVMIVASVVTVSDNGL
                     AFTAIAEIAGPFWSGRALGTQNTSQLLTAGIAPPVFGGLIAAAGYSAAFAACAVFPLL
                     ALPLVPTRAAATGLPERGG"
     gene            2924..3703
                     /locus_tag="VF874_22230"
     CDS             2924..3703
                     /locus_tag="VF874_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9178680.1"
                     /translation="MRLGRIASPDGVAFVSIEGNDGDAIVREIAEHPFGTPTFTGRRW
                     PLADVRLLAPILASKVVCVGKNYAAHIEEMGGGQFEDPVIFMKPNTAIIGPNVPIQLP
                     SNANPVHFEGELAAVIGRPCKDVPAARAAENILGYTVANDVTARDQQRKDGQWTRGKG
                     HDTFCPVGPWIVTDVDPSDLEIRTEVNGEVRQHSRTSLMIHDVGAIVEWISAVMTLLP
                     GDLILTGTPEGVGPIEHGDTVSVSIEGVGTLTNPVVRKADS"
     gene            3703..5166
                     /gene="gltX"
                     /locus_tag="VF874_22235"
     CDS             3703..5166
                     /gene="gltX"
                     /locus_tag="VF874_22235"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX9178681.1"
                     /translation="MVRVRFCPSPTGVPHVGLIRTALFNWAYARHCDGVFVFRIEDTD
                     SQRDSEESYLALLDALRWLGLDWDEGPEVGGPYGPYRQSERLDIYRDVLARLLGTGQA
                     YEAYSTPEEVEARHLAAGRNPKLGYDNFDRDLTEEQRDAYRAEGRRPVVRLRMPDGEL
                     GWQDLVRGEMSYAAGSIPDFALTRGNGEPLYTLVNPVDDALMKITHVLRGEDLMPSTP
                     RQIALYQALQRIGVADHLPEFGHLPTVLGEGTKKLSKRDPQSNLFMHRERGFIPEGLL
                     NYLALLGWSIASDRDVFSLEEMVRAFDVADVNANPARFDQKKADAINAEHIRLLDPDD
                     FTARLRDHFAAHGHDLGLDDARFTEAAGLVQTRIVVLGDAWGLLKFLDDDAYVIEEKA
                     ASKELGADAAPVLDAALAALDGVGDWSVPHIEAALTATLIDEMGLKPRKAFGPIRVAV
                     TGSTISPPLFESLELLGRDRSLSRLRDARGLVAGAEA"
     gene            5261..5332
                     /locus_tag="VF874_22240"
     tRNA            5261..5332
                     /locus_tag="VF874_22240"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5294..5296,aa:Gln,seq:ctg)
     gene            5394..5466
                     /locus_tag="VF874_22245"
     tRNA            5394..5466
                     /locus_tag="VF874_22245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5428..5430,aa:Glu,seq:ctc)
BASE COUNT          933 a         1888 c         1990 g          932 t
ORIGIN      
        1 actcgccgca gccggtgcag cggtcgtaat cgaaccggta ccggtcgccc ttctggccca
       61 gcttgatgac cgcgtcctcc gggcaggcgc ccaggcaacc gtcgcactcg aagcagttgc
      121 cgcacgacag gcaccggcgc gcctcgtacg tcgcggccgt ggcgttcagg ccgcccacga
      181 cctcttcgaa tccggcgact cgctcgtcgg gcggcagctc gggctgtgcg cggcgggctg
      241 cgtcgccgaa gtaccacagg tgtagctcgt cgaatgtcgc gatcggatgc ttgggcggcc
      301 gctcgtccga ggtgtcccgc agccacgcgt cgatgtggtg cgcggccttc ttgccgtggc
      361 cgacgccgac ggtgacggtg cgctcgcagg gcaccatgtc tccgccggcg aacacgccgg
      421 ggtagcccgt catcagcgtc tcggacacct gcacggaccc gtcgtcgtcg aacaccacgc
      481 cgggtaggtt gcgcatgaac gccgactcgg tttcctggcc caacgccatg atcacggtgt
      541 cggcggcgag cgtctcgtac cgcccggtcg ggtgaggctt gccgttgtcg tcgagttcca
      601 tgacctcgac ctgcatctcg tgccggtcga aggcggcgat ggtgcgcagc cagtggatct
      661 gcacgccctc gcgctcggcc tcctcggcct cctcctcgtg cgcgggcatc tgcgcgcggg
      721 tgcggcggta gatgatgacg gtgtcgtcgg cgccgagtcg gcgcgccacc cgggcggcgt
      781 ccattgcggt gttgccgccg ccgtacaccg ccacgcgggg cccgatttct ggatgctccc
      841 cggaggcgac gctgcgcagg aacgccaacg cgtcgaccat cgtcgacgcg tcgtcgaccg
      901 ggatgttgac ccgcttggcc aggtgggcgc cgaccgcgac gaacgccgcg tcgaactggc
      961 ccgtctggag ttcgtcggcg aggtcttcga cccggtgccc gcaggtgatc tgcacgccga
     1021 gcgcagcgat gcgccggacc tcgtcgtcaa gtacgtcgcg ggggagccgg tagttgggga
     1081 tgccgtaacg catcatcccg cctggcacgg cgccggcgtc gcggatctcg acggcatgcc
     1141 cccgccgggc caggtggtag gcggccgaca gcccgctggg gccggcgccg acgatcagga
     1201 cctttttccc ggacggctcc gggggtggtg cgaactgcca gttgttttcg cgtgccgcat
     1261 cgccgaggaa ccgctcgacc gcgtggatgg acaccgccga gtccaggttg gcgcggttgc
     1321 agttcgactc gcacgggtga tagcacaccc ggccgtggat cgcagcaaaa ggattgtccg
     1381 ccaccaggag tcgccacgcc tcctcgtgct tgccgacgcg ggcgtggccg agccagccct
     1441 ggatgttctc gccggccggg caggcgttgt tgcacggggg cagtaggtcg acgtagacgg
     1501 ggcgccgatc gcgcaccggc ccggcgcgtg gccgcccgtg cagcagatcg ggcagagggg
     1561 tgaggtcgtg ctcgactccg ggcacatctt cggacgtcac ttttctcaca ataggccgat
     1621 tggtgtcggt tagccgccgc gttcgggtaa acccgttgcg gccgcgcgcg tcggaaccag
     1681 cggcagggcc agcagcggga agacggcgca cgcggcgaac gccgccgagt acccggctgc
     1741 ggcgatgagc ccaccgaaga ccggtggcgc aatcccggcg gtcagcagtt ggctggtgtt
     1801 ctgcgtgccc agtgcgcggc cgctccagaa cggcccggcg atctcggcga tcgcggtgaa
     1861 cgccagaccg ttgtcgctga ccgtgaccac cgatgcgacg atcatcaccg cgacggccag
     1921 cggcgaatgc cagtagtcgg tgaccgccaa cagacccatc gtgagtgcag ccgccgcggc
     1981 gatcgtcctg atgggacgca gccgtgaccc gacgcggtcc gaccatcggc ccgccgcgac
     2041 gcggcccagc gcgccgagaa cctgtgctgt tgtgaccagc gcccccgcgg agcgggccga
     2101 ccagccccgg tcggtcatca gccacaccag cgtgaaggtc cacaccacca cttgcggcac
     2161 gaccagcagc accgaaacgg cgtggatgcg ccacaacacc gacgatcctc ggtacgggtt
     2221 ggcgaggtct tcctgcgggg cctgttcgcg cggcggacgt ggcggatcga cgacgccgac
     2281 ggcgctgacg atcgccgaca ctgcgcacac gacggcgggg aacagcagcg ccgtaccgat
     2341 cccgtggttt tgcgccagct gcggaatcac cagggcgccc aacccgactc cgagcggctg
     2401 cgcggtctgc cggatgccca tcgccagccc gcgcctctcg gcggggaacc agccgaccac
     2461 gagccggccg ctggcggagt tgctgctggc cgcggccatt ccgccgagca gcaggaaagt
     2521 gcccgtgagc attattgagt tcgacgccgc ggggcctgat gtcgccgccc cggcggctcc
     2581 agcggcgaac gcggcggccg cggtgagtgc ggagccgagc gcgagcacca ggcgctcgcc
     2641 gagacggtcg actgcgtatc cccacgcgat cagcgtgaac accatgccga tgctgggcat
     2701 cgcggacagc aggccggcgt cggcgaggtc cagcccgcgg cgctcatgca atgtgggaat
     2761 gagaaacgcg gcgccgttta tgaacacgtt ggcgaacatc gtcgaggaca acgcgattgc
     2821 gagcatcgac caccgtcggg ctgtgccgat ggagtcgacg ctcaccgact catcgtcgca
     2881 ccgccgcacg atcatcggca cggctgccac taggctttca caaatgcggc tcggtcgaat
     2941 cgccagcccc gatggggtgg cgttcgtcag catcgaaggg aatgacggcg acgcgatcgt
     3001 ccgcgagatc gccgagcacc cgttcggaac gccgacgttc accgggcggc ggtggccact
     3061 ggccgacgtg cggctgctgg caccgattct ggcgagcaag gtggtgtgcg tcggcaagaa
     3121 ctacgcggca cacatcgagg agatgggcgg tggacagttc gaggatccgg tgatcttcat
     3181 gaagccgaac acggcgatca tcgggcccaa tgtgccgatc caactgccgt cgaacgcgaa
     3241 cccggtgcac ttcgagggtg aacttgccgc ggtgatcggc cgcccctgca aagacgttcc
     3301 ggccgcacgt gctgccgaga acatcctcgg ttacaccgtc gccaacgacg tgacagcgcg
     3361 tgaccaacaa cggaaagacg ggcagtggac acgcggcaag gggcacgaca cgttctgtcc
     3421 ggtggggccg tggatcgtca ccgatgttga cccgtcggat ctcgaaattc gcaccgaagt
     3481 gaatggtgaa gtgcgccaac acagccggac gtccctgatg atccacgacg tcggcgcgat
     3541 cgtcgaatgg atctcggcgg tgatgaccct gttgcccggt gacctgatcc tgaccggaac
     3601 accggagggg gtgggtccga tcgagcacgg cgataccgtc agcgtcagca tcgagggcgt
     3661 cggaacgctg acgaatcctg ttgtacgcaa ggctgattca taatggtccg cgtccggttc
     3721 tgcccgtcac cgaccggcgt accgcacgtc ggcctcatcc gcaccgcgct gttcaactgg
     3781 gcctacgcgc ggcactgcga cggcgtcttc gtgttccgca tcgaggacac cgactcccag
     3841 cgcgacagcg aggagagcta cctcgcgctg ctcgacgcgc tgcgctggct cgggttggac
     3901 tgggacgaag ggccggaagt cggtgggccg tacggcccgt atcgccagtc ggagcgtctc
     3961 gacatctacc gcgacgtgct ggcgcggctg ctggggacag ggcaggccta cgaggcgtac
     4021 tcgacgcctg aggaggttga ggcgcggcat ctggccgccg gccgcaatcc caagctgggg
     4081 tacgacaact tcgaccgcga cttgaccgaa gagcagcgcg acgcgtaccg cgccgagggg
     4141 cggcggccgg tggtgcgact gcgcatgccc gatggcgaac tcggctggca ggacttggtg
     4201 cgcggcgaga tgtcgtacgc cgcgggcagt attccggact tcgcgctgac ccggggcaac
     4261 ggagaaccgt tgtacacgtt ggtcaatccg gtcgacgacg cgctgatgaa gatcacccac
     4321 gtgctgcgcg gcgaagacct gatgccgtcg acgccgcgcc agatcgcgtt gtaccaggcg
     4381 cttcagcgga tcggcgtcgc cgaccatctt cccgagttcg gtcacctccc aacggttctc
     4441 ggcgaaggca ccaagaagct ctccaaacgc gacccgcagt cgaatctgtt catgcaccgc
     4501 gagcgcgggt tcatccccga gggtctgctg aattaccttg cgctgctggg ctggtcgatc
     4561 gcttccgatc gcgatgtgtt cagcctcgag gagatggtcc gcgcgttcga cgtcgccgac
     4621 gtcaacgcga acccggcacg gttcgaccag aagaaagccg acgcgatcaa cgccgaacac
     4681 atccggctgc tcgatccaga cgacttcacc gcacggctgc gcgaccactt tgccgcgcac
     4741 ggccacgatc tcggactcga cgatgcgcgg ttcacggagg ccgcagggtt ggtgcagacg
     4801 cgcatcgtgg tgctcggcga tgcatggggg ctgctgaagt ttctcgacga cgacgcctac
     4861 gtaatcgagg agaaggctgc gtctaaggaa ctcggtgcgg atgctgcgcc ggtgctggat
     4921 gctgcgctgg ccgcgctcga cggcgtgggg gactggtcgg ttccgcacat cgaggccgcg
     4981 ctgacggcga cgctgattga cgagatgggt ctcaagccgc gcaaggcatt cggaccgatt
     5041 cgggtcgccg tgacgggttc gacgatcagc ccgccgctgt tcgagtcact ggagctgctg
     5101 ggccgcgacc gcagcctttc tcggttgcgc gacgcccgcg ggctcgtggc cggcgccgag
     5161 gcatgagaaa gccggccggg ttgtttggta gtctgcacgt cggcccgtgc atgcggctgg
     5221 cagcctgctt atggctctga ccagcggtta tgacggccaa tggggtatgg tgtaattggc
     5281 aacacagctg attctggttc agccattcta ggttcgagtc ctggtacccc agctcatcgc
     5341 gaattggggt ccgtcttgcc gcgtcggtta tgctgacaag ccggattgtt catggccccg
     5401 tcgtctagcg gcctaggacg ccgccctctc acggcggtag cgtgggttcg aatcccatcg
     5461 gggctacaaa tccgcgagca gacgcaaagg cccccatttc ctgcggaaat gggggccttt
     5521 ccgtctgctg ggacgcgggc agctgcgcgc aggttgacgt cgaggttgtt ggaaccaccg
     5581 gaagcactgc gtcgagacgc tgaacacaag gtgcctccgc ccgccgcgcc gtctagcaca
     5641 tcggtccgac aagtgcgact gtgcgaatgg tgtttgtcca cagggatttt cgggtgtgga
     5701 taggtccgga acttgtcggc gggcggggat agaattcgct cat
//
LOCUS       DASWBF010000600         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_657599,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000600 DASWBF010000000
VERSION     DASWBF010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657599"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VF874_22250"
     CDS             complement(<1..719)
                     /locus_tag="VF874_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178682.1"
                     /translation="MANQLDQTLQPTVSVMGNLRRVRDGAIWADYRLTGLPYGYTSDE
                     RKYDTLVHHKNLIRALPNNAVIAGLIAAMNPDEMLARAIAGTDVNAHPMWREECEGKH
                     EFFTSTVRATERIFVMSFPVTDSGVVTDLTGWGATGRRSNERDRAEMARAAQSAAAIV
                     SRLPSVFELQPLTPSQMVWLWNRALSRGGAAEVFPTSVPSNVSSPAGAFAMAEFDEGE
                     RRREVKRWRPSSFAPLVKITQP"
     gene            complement(730..1260)
                     /locus_tag="VF874_22255"
     CDS             complement(730..1260)
                     /locus_tag="VF874_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178683.1"
                     /translation="MFVAKWYTRASRVRIVIAKFDGRWKLPGGPYPIPELVALVGGIL
                     LTLFLLPRVGHPVFTGAVGVTITAVAVGAMRKMSYSPVKFSTRMHRIMALYTSPVSIS
                     TGNDARAVDTVSTVRGDVEFLDVLADDPAPVYAPHHACLAPVRELEPDGWADMFDRPR
                     SGAAAELFGLTGNPAI"
     gene            complement(1273..1590)
                     /locus_tag="VF874_22260"
     CDS             complement(1273..1590)
                     /locus_tag="VF874_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178684.1"
                     /translation="MIATIAPPVLDSAAAGAATHVTTVAAPIWDIGANIKNQGITFAV
                     YLLIGGTALIAAGYYFIAKNKTAALKTLAVGVVLIGLVGSLPALGVVSKDTIGGLTNT
                     GTR"
     gene            complement(1594..2607)
                     /locus_tag="VF874_22265"
     CDS             complement(1594..2607)
                     /locus_tag="VF874_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178685.1"
                     /translation="MLTRPLHRVLDSVFLRLSGGDTIRADELRMRATGSFLAFIIISY
                     IPVVLAVALLVLNRPTAVNEAAERDQLAAIEAYATRYVDSYLKDPSNTAAIKQFYDGD
                     IPASALPAGGRALRASSALPGVFADGFRTYSVVVDAEIPKAANSASMVYVRLQVDISA
                     DRQNYFRAFTLPHARPDRPVGQPVELATQTLISEDRPVYKTVSGFLSAMLIGQGELAP
                     YIAAGSTLTAAQPRRFTTMAIERVLTNSEVANAQDVPPKANGVEVTVRAVMQTASGVS
                     MPMDFPLVMSVAGGHWQVDRINDAPSVVAPADAGPSPPATTTPTTTTTTPPMYAPNTT
                     EGN"
     gene            complement(2601..2801)
                     /locus_tag="VF874_22270"
     CDS             complement(2601..2801)
                     /locus_tag="VF874_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178686.1"
                     /translation="MALPIRRTATAVRAYSEQLQDLMDQLAADPLDEMKSIALVAHIV
                     NNRGPAAQLLDVLQTQRIGAPC"
BASE COUNT          545 a         1010 c         1030 g          519 t
ORIGIN      
        1 ggctgggtga tcttcaccag cggcgcgaag ctactgggcc gccaacgctt cacctcccgg
       61 cgccgctcac cctcgtcgaa ctcggccatc gcgaacgcac ccgccggcga ggacacattc
      121 gacgggaccg atgtcgggaa cacctcggcc gccccgccgc gcgacagcgc ccggttccac
      181 agccacacca tctgcgacgg cgtcagcggc tgcagctcaa acaccgacgg cagccgtgac
      241 acgatcgcgg ccgccgactg cgcggcccta gccatctcgg cgcggtcgcg ctcgttggac
      301 cgccgcccgg tcgcacccca tcccgtcaaa tcggtgacca caccggaatc ggtcaccggg
      361 aacgacatca cgaagatccg ctccgtcgcg cgcaccgtcg aggtgaaaaa ctcgtgtttt
      421 ccctcgcact cctcacgcca catcgggtgc gcattgacat cagtgccggc gatggcgcgc
      481 gccagcatct catcggggtt catcgccgcg atcaggccgg cgatcaccgc gttgttcggc
      541 aacgcccgaa tgaggttctt gtgatggacc agggtgtcgt acttgcgttc atcgctggtg
      601 tatccgtagg gcagcccggt gaggcggtaa tcggcccaga tcgcgccgtc acgcacccgc
      661 cgcaaattgc ccatcaccga caccgtcggc tgcagggtct ggtcgagctg gttggccatg
      721 gcgacgtcct cagatggcgg ggtttccggt caaaccgaaa agctcggcgg ccgcgccact
      781 gcggggccga tcgaacatgt ctgcccaacc gtcgggttcc agttcacgaa ccggggccag
      841 gcaggcatga tgaggcgcgt acaccggtgc cgggtcatcg gctagcacgt cgaggaattc
      901 gacatcgccg cgcaccgtgg acaccgtgtc aacggcgcgg gcatcgttgc cggtgctgat
      961 gctgacggga ctggtgtaga gggccatgat gcggtgcatg cgggtgctga acttcaccgg
     1021 cgagtacgac atcttgcgca tcgctcccac cgcgacggcg gtgatcgtca cgccgacggc
     1081 gccggtgaat accgggtgac ccacccgcgg cagcaagaac agcgtgagca gaatgccgcc
     1141 aaccagcgcc accagttccg ggatcgggta ggggcctccg ggtagcttcc accgaccgtc
     1201 gaactttgcg atgacgatgc gcacccggct ggcccgggtg taccacttgg ccacgaacat
     1261 gcgcgctgcg acctaccggg ttccggtgtt ggtgagtccg ccgatggtgt ccttggacac
     1321 cacgcccagt gcgggcagtg agccgaccaa accgatcagc accacaccga ccgccagcgt
     1381 cttcagggca gccgtcttgt tcttcgcgat gaagtagtag ccggccgcta tgagcgcggt
     1441 gccgccgatc agcaggtaca ccgcaaaggt gatgccctgg ttcttgatgt tggcgccgat
     1501 atcccaaatc ggtgccgcca ccgtggtgac atgggtggcc gctcctgcgg cggcgctgtc
     1561 gagcaccggg ggggcgatgg tggcgatcat ttgttagttc ccttcggtgg tgttgggggc
     1621 gtacatcggt ggggttgttg tggtcgtggt gggagtcgtc gtcgcgggtg gcgatggacc
     1681 ggcgtcggcg ggcgccacga cgctgggggc atcgttgatg cggtcgacct gccagtgccc
     1741 gccggcgacg gacatgacga gcgggaaatc catcggcatc gacacgccgc ttgcggtctg
     1801 catgacggcc cgaacggtga cctcaacacc gttggctttc ggcggcacat cctgtgcgtt
     1861 ggcgacctcg gaattggtca gcacacgctc gatcgccatg gtcgtgaaac gtcgtggttg
     1921 agcggcggtc aaggtcgatc cagccgcgat gtatggcgcc agctcgccct gcccgatcag
     1981 catcgcgctg aggaaccctg agaccgtctt gtacaccggg cggtcttcgg agatcagcgt
     2041 ctgcgtcgcc aactccaccg gttggcccac cgggcggtcc gggcgggcgt gcgggagggt
     2101 gaaggcacgg aagtagttct gacgatcggc tgagatgtcg acctgcagcc gcacatacac
     2161 catcgacgcc gagttcgcag ccttgggaat ctcggcgtca acgaccaccg aataggtgcg
     2221 aaacccgtcg gcgaagacac cgggaagagc cgaggatgcc cgcagcgcgc gaccaccggc
     2281 cggcagcgca gacgcgggga tatcgccgtc atagaactgc ttgatggctg cggtgttgga
     2341 gggatctttg aggtagctgt cgacgtaacg ggtggcgtaa gcctcgatgg ccgctagttg
     2401 gtcgcgttcg gcggcttcgt tcaccgcggt cggccggttc aacaccagca gcgcgaccgc
     2461 gagcacaacc gggatgtagc tgattatgat gaacgccaga aacgacccgg tcgcccgcat
     2521 ccgcagctcg tccgcgcgga tcgtgtcacc gccgctcaga cgcaggaaca ccgagtccaa
     2581 gacccggtgc aggggacggg tcagcatggg gccccgatgc gctgggtctg cagcacgtcc
     2641 aacagctggg cggccgggcc gcggttgttg acgatgtggg cgaccagcgc gatcgacttc
     2701 atttcgtcga gaggatcggc cgccaactga tccatcaggt cctgcagctg ctcggagtac
     2761 gcgcggaccg cggtggcggt gcggcgaatc ggcagcgcca tcggcgggcc tccacaggaa
     2821 tcggggtgct cggacaccct tctatgcggg gtaaatcacc gattccgcga caaccgcata
     2881 ggtaacgaat tgataacgca tatgtgcgta tgccatcgcg atgttcgcta gatgcttgac
     2941 aaggggctta cgctcggctc gacgagcccc aggcgaggag gaagccgagc ggcacccctt
     3001 accgacttag ctcaccgcgc atgcgccgag cgcatcagcg cctattccgc gccaccaaac
     3061 caggccgcat cacccgcgca tcccccgtga cgccatccgc ggat
//
LOCUS       DASWBF010000601         4934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_328847,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000601 DASWBF010000000
VERSION     DASWBF010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328847"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..309
                     /locus_tag="VF874_22275"
     CDS             115..309
                     /locus_tag="VF874_22275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178687.1"
                     /translation="MKTVVAIVGGFVVVGMGVLTVVVGGTEAHAKVLGGSGDTVTQTT
                     PTTLASWSASPTVKAKPPSA"
     gene            376..651
                     /locus_tag="VF874_22280"
     CDS             376..651
                     /locus_tag="VF874_22280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178688.1"
                     /translation="MNAEQRNQRNLKIGAAAFGAGVLIAMGAITAATSGDAITTSEPP
                     TGPTDSATSSTSTTPPSTRETTKASPEVTATTSEGSWPETSTVELPS"
     gene            723..1157
                     /locus_tag="VF874_22285"
     CDS             723..1157
                     /locus_tag="VF874_22285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178689.1"
                     /translation="MKLPKQAPPRNVYLSAAQLDMLASEAGPYRSLVLLLGVGGLRWG
                     EAIALRVCDVDFLHRRVELHRNVVRVRSEFVIGSLKSNKNRVVVVPSFVVDALAQTAA
                     GKGREDLLWTAPFGGYLRTPGRESWLAGAVARCQVADETFPV"
     gene            1154..1366
                     /locus_tag="VF874_22290"
     CDS             1154..1366
                     /locus_tag="VF874_22290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9178690.1"
                     /translation="MTAHDLRHTAASLAISAGANPKVVQRMLGHSSAAMTLDVYADLF
                     ECDLDAVAESVSKCVHSGPERTPVRP"
     gene            1397..1894
                     /locus_tag="VF874_22295"
     CDS             1397..1894
                     /locus_tag="VF874_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX9178691.1"
                     /translation="MTVTDEYLANNAEYAKTFQGPLPLPPSKHVAVVACMDARLDVYR
                     ILGLDDGEAHVIRNAGGVVTDDEIRSLAISQRLLGTKEIILIHHTDCGMLTFTDDDFK
                     RSIQDETGIKPEWAAEAFSDLEQDVRQSIRRIEASPFVTKHESLRGFVFDVATGKLNE
                     VTLDS"
     gene            complement(1900..2283)
                     /locus_tag="VF874_22300"
     CDS             complement(1900..2283)
                     /locus_tag="VF874_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9178692.1"
                     /translation="MSIAFNHTIVAAKDKGESAAFFTELFGLPPAKPFGPFLAVELDH
                     GVSLDFIDSGDDVRPQHYAFLVSEADFDAIYGRIRERGMEHWADPHGHRPGEINHNDG
                     GRGVYFCDPGGHYLEIITRPYGSGS"
     gene            2298..2828
                     /locus_tag="VF874_22305"
     CDS             2298..2828
                     /locus_tag="VF874_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9178693.1"
                     /translation="MPDGDVLLGPQILLRAPKVEDAEDLFASVTSDPNVTEYLSWTPH
                     PDVDETLRVITEMFNVGDDHTWLIALRDSGEVIGELGYRQPQRHAVNIGYCLARQWWG
                     RGIMSEAVSLLLQQLQQDSSVFRASAACHVDNVGSARVLERCGLSLEGRLVRYLVFPN
                     LGPEPQDCLLYARAMR"
     gene            complement(2829..3596)
                     /locus_tag="VF874_22310"
     CDS             complement(2829..3596)
                     /locus_tag="VF874_22310"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9178694.1"
                     /translation="MFSASDGYERFMGRWSRELAPMLVEFAGTAPGDAVLDVGCGTGA
                     LSVAAAAVSRVRVIGVDPSDDFVRFATSHVRTGRIRFEVGQASALSFPDGQFDRTLSL
                     LVLNFVPEPGAAVREMIRVTRSGGVIAAAVWDYASGMQMLREFWHAAVELDPAAAPRD
                     ERHMPLCGDGELAELLKLHRLTNVEQRELSIEMRFASFDDYWQPFLAGQGPAGAYTIS
                     LPDAARNTLEGNLRQRLSSSGAFTLNARARAVRGVVT"
     gene            3638..4660
                     /locus_tag="VF874_22315"
     CDS             3638..4660
                     /locus_tag="VF874_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9178695.1"
                     /translation="MSAFQFADLAVPVLAAPMAGGPSTPELAAAATNAGGLGFVPAGY
                     LSADDFAERLVAARAMASGPLGANLFVPQPRAGNRADIDRYAQVLAGEAQRYGAQLGE
                     PRYDDDNWSAKLDVLLDIRPEVASFTFDAPSAGEIRRLADAGITTVVTVTTASEAELA
                     VSRGADALVVQGPSAGGHRGTFDPAARPATQPLSELLAEVVSRVAMPVVAAGGLMTAE
                     EVDAALRAGAVAAQLGTAFLLSDEAGSSPVHRMALQDPQFTETVVTHVFSGRYARGLR
                     NRFIEEHEAQAPFAYPQVHYLTGPLRAAAVRAGDPHATNVWAGTGFKKAKAASVAAIM
                     GELSCR"
     gene            4663..>4934
                     /locus_tag="VF874_22320"
     CDS             4663..>4934
                     /locus_tag="VF874_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9178696.1"
                     /translation="MTMPVMEPDLDTATLRAWARATDEGPFASACFGERIAFDNPETL
                     TLLGALAAWTDRVRLVTTVIVPQLHDPVMLAKALATGDMLSGGRLTV"
BASE COUNT          858 a         1625 c         1647 g          804 t
ORIGIN      
        1 ttttcacagc cgtgcggtgt tcgcttcgac ctcgaagaca gtcatctggg tcggccgcgc
       61 agcgtggggc gagcgaccag gcaccggatt ggagttacag agatgaaatc gcagatgaag
      121 acagtagtgg cgatcgtcgg ggggttcgtc gtcgtcggga tgggcgtgct gaccgtcgtc
      181 gtgggcggca ccgaggcgca cgcgaaagtg ctcggcggct ccggcgacac cgtgacgcag
      241 accacgccca ccacactggc aagctggtcg gcgtccccga cggtgaaagc aaagccgccg
      301 agcgcgtagc cacgtttcgt tccggctagc gccgttaccc actccgtcag acctagtcga
      361 acggagtggt ccgacgtgaa tgcggaacag cgaaaccagc ggaacctgaa aatcggtgcg
      421 gcggcgttcg gtgctggcgt cctcatcgcg atgggcgcga tcacggccgc gaccagcggc
      481 gacgccatca ccacctcgga acctcccacc ggcccgactg acagcgccac cagcagcacg
      541 tcaacgacgc cgccgtcgac gcgggaaacg acgaaggcaa gccccgaggt caccgcgacc
      601 acgtcagaag gatcgtggcc ggagacgtcg acggtcgaac tgccgtcata agcggcaaga
      661 atctgcctga tcgtgacccc gggacgccta gggcgaacgg cgcggcgaac cccgcacggg
      721 gtgtgaagct gcccaagcag gcgcccccac gcaatgtgta tctgagtgcg gcgcaactgg
      781 acatgctcgc cagcgaggcg gggccctacc gctcgctggt gctgctgctg ggtgtcggcg
      841 ggctgcgctg gggtgaggcc atcgcgctgc gggtctgcga cgtcgacttc ctgcaccgcc
      901 gcgtcgagct gcaccgcaac gtggtgcggg tgcgcagcga gttcgtcatc ggcagcctca
      961 agagcaacaa gaaccgcgtc gtggtggtgc catcgttcgt cgtggacgcg ctggcgcaga
     1021 ccgccgccgg taagggtcgc gaggatctgc tatggaccgc gccattcggc ggctacctcc
     1081 gtacccccgg ccgcgaatcc tggctcgccg gtgcggtggc gcgctgccag gtggccgatg
     1141 agacgttccc cgtgtgaccg cgcacgacct gcggcacact gcggcctcgc tggccatctc
     1201 ggcgggcgcg aaccccaagg tggtgcagcg catgctgggg cactcctcgg cggccatgac
     1261 gctggacgtg tacgccgatc tgttcgagtg tgacctggac gcggttgccg aaagtgtgtc
     1321 caaatgtgtc cacagtgggc ctgagcgcac ccctgtccga ccctgaaaac gtctctacca
     1381 gcgaataagg agccccatga ccgtgaccga cgaatacctg gccaacaacg ccgagtacgc
     1441 caagactttt caagggccgt tgccgctgcc gcccagcaag cacgtcgcgg tcgtcgcgtg
     1501 tatggacgcc cggctcgacg tgtaccgcat tctcggcctc gacgacggcg aggcgcatgt
     1561 gatccgcaac gccgggggcg tcgtcaccga cgacgaaatc cggtcactgg ccatcagcca
     1621 gcggctgctc ggcaccaaag agatcattct cattcaccac accgattgcg gcatgctcac
     1681 cttcaccgac gacgacttca agcggtcgat tcaggacgaa accggcatca aacccgaatg
     1741 ggccgcggaa gcgttttccg acctcgaaca ggatgtgcga cagtcgatcc gccgcatcga
     1801 ggcaagcccg ttcgtcacca agcacgagtc gctgcgcgga ttcgtcttcg acgtcgcgac
     1861 cggcaagctg aacgaggtga cgctggacag ctgagcgcct cagctgccgg aaccgtaagg
     1921 ccgcgtgatg atttccaggt agtggccgcc gggatcacag aagtacacgc cgcgaccgcc
     1981 gtcgttgtgg ttgatctcgc cgggccggtg gccatgcgga tcggcccaat gctccattcc
     2041 gcgctcacgg attcgcccat agatcgcgtc gaagtcggcc tccgagacca gaaatgcgta
     2101 gtgctgcggc cgcacgtcgt cgccgctgtc gatgaaatcg aggctcacgc cgtgatcgag
     2161 ttccaccgcc aggaacgggc cgaagggctt ggcgggtggt agcccgaaga gttcggtgaa
     2221 gaaggcggcc gactctccct tgtccttggc cgccacgatg gtgtggttga acgcgatgga
     2281 catgacacag tatgggcatg ccggacggcg acgttctact aggtccgcag atcctgttgc
     2341 gtgcacccaa ggtcgaggat gccgaggacc tgttcgcgtc cgtgacctcg gacccgaacg
     2401 tcaccgagta cctgtcgtgg acgccgcatc ccgacgtcga cgagacgttg cgggtgatca
     2461 cggaaatgtt caacgtcggc gacgaccata cctggcttat cgcgctgcgc gattccggag
     2521 aagtgatcgg cgaactcgga tatcggcagc cacaacgcca tgcggtgaac atcggatact
     2581 gtctcgcccg gcagtggtgg ggccgcggca tcatgtcgga ggcagtctcg ctgcttttgc
     2641 aacagttgca gcaggattcg tccgtctttc gagcgtcggc ggcatgccat gtcgacaacg
     2701 tgggctcggc ccgggtgctc gagcgctgcg gcttgtccct ggaaggccgg cttgtccgct
     2761 atctcgtctt tcccaacctg ggtcccgagc ctcaggactg cctgttgtac gccagggcga
     2821 tgaggtgact acgtgaccac tcctcgaacc gcccgagcgc gggcattgag tgtgaacgct
     2881 ccgctggagc tcagtcgttg gcggaggttg ccctcgagag tattccgcgc ggcgtcgggc
     2941 agtgaaattg tgtacgcacc tgcgggacct tgtccggcga ggaatggctg ccagtagtcg
     3001 tcgaacgatg caaaacgcat ctcgatcgat agctctcgct gctcgacgtt ggtcagcctg
     3061 tgcaacttca gcaactcggc cagctcgccg tccccgcaca ggggcatgtg gcgttcgtcg
     3121 cgcggcgcgg ctgctggatc caattcgacg gcggcgtgcc agaactcgcg gagcatctgc
     3181 atgcccgacg cgtaatccca cactgccgcg gcgatgaccc cgccagatcg tgtcacgcgg
     3241 atcatctccc gcaccgctgc gcccggctcg ggcacgaagt tcaacaccag cagcgacagc
     3301 gttcggtcga actggccatc aggaaagctg agagcacttg cctgaccgac ttcgaatcgg
     3361 attcgaccgg ttcggacatg gctggtggcg aagcgaacaa agtcgtccga cggatccaca
     3421 ccgatgacgc gaacgcgtga aaccgcggcg gctgcgacgc tcagcgcacc ggttccacag
     3481 ccgacgtcga gcaccgcgtc ccccggggcg gtgccggcga actcgaccag catcggcgcc
     3541 agttcgcggc tccagcgacc catgaatcgc tcgtaaccgt cgctcgctga gaacatgtct
     3601 tgtcagcgta tcccgcctga ggtgatagga agcagcgatg agcgctttcc agttcgccga
     3661 tctcgccgtg ccggtgcttg ccgcgccgat ggcgggtgga ccgtccacac cggagctggc
     3721 agccgccgcg accaacgccg gcgggctggg gttcgtgccc gccggatatc tgtcggccga
     3781 cgacttcgcc gagcgtctcg tcgccgcccg ggcgatggcg tcgggtccgc tgggagcgaa
     3841 tctctttgtg ccccaacctc gcgcgggcaa ccgagcggac atcgaccggt acgcccaggt
     3901 gttggccggc gaggcgcagc gctacggtgc acagcttggt gaaccgcgct acgacgacga
     3961 caactggtcg gccaaactcg acgtgctgct cgacatccgc cccgaagtcg cgtcgttcac
     4021 cttcgatgcg ccgagcgccg gggagatccg gcgtctggcg gacgcgggca tcacgaccgt
     4081 cgtcacggtg accaccgcat ccgaagccga gctcgcggtg tcccgcggcg ccgacgcgct
     4141 ggtggtgcag gggccatcgg ccggcgggca ccgcggcacg ttcgaccccg cggcacggcc
     4201 ggccacgcaa ccgctgtcag agctgctggc agaagtggtc tcccgcgtcg ccatgccggt
     4261 ggtggcggcg ggtgggttga tgacggccga ggaggtcgac gccgcgctcc gcgccggcgc
     4321 ggtggccgcg cagctgggca ccgcgttcct gctgtccgac gaagcgggca gcagcccggt
     4381 tcaccgtatg gccctgcagg atccgcagtt caccgaaacg gttgtgacac atgtattttc
     4441 gggccgctat gcccgcggct tacgcaaccg gttcatcgag gaacatgagg cgcaggcccc
     4501 gttcgcgtat ccgcaagtcc actacctgac cggcccgctg cgcgcggcag cggtacgcgc
     4561 cggcgacccg cacgccacga acgtctgggc cggaacggga ttcaagaagg cgaaggccgc
     4621 gtcggtggcc gcgatcatgg gagagctgtc gtgcaggtag gcatgacgat gccggtgatg
     4681 gaaccggatc tggacaccgc gaccctgcgg gcctgggcgc gcgcgaccga cgaaggcccc
     4741 tttgcctcag cgtgtttcgg tgaacggatc gcgttcgaca accccgaaac actcacgctg
     4801 ctgggcgcgc tcgccgcgtg gaccgaccgg gtacggctgg tgaccaccgt gatcgttccg
     4861 cagctgcatg atccggtcat gctggccaag gcgctcgcga ccggcgacat gctcagcggc
     4921 ggccgcctga ccgt
//
LOCUS       DASWBF010000602        13025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1117049,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000602 DASWBF010000000
VERSION     DASWBF010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13025
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117049"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..3292
                     /locus_tag="VF874_22325"
     CDS             350..3292
                     /locus_tag="VF874_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9178697.1"
                     /translation="MMRLSSNLRRYRWAVFTAWLLVLLPAVLIGLHESNNLTGGGFEV
                     AGSQSLQVQEQLERHYPDQGASPLALVAAPRPGATVDDMTAAVDLLEQKVAEVPSVSL
                     APNPPAQPPPGPERPYVVSLKLGFNNSGAVDVAKQLRSKVGINGDKPGQLDNGKVDLY
                     VIGQGALGAAAQEKTRHDIGEAEQWGLPIILIVLLAVFGSLAAAAMPLILGVLTVVVS
                     MGVVFLLSMFTTMSVFVTPTVSMFGIALAVDYSLFILMRYREELRAGRDAKQATDAAM
                     ATSGLAVVLSGLTVIASLAGIYLINTPVLRSMATGAVVTVAVAVLTSVTLIPAILATF
                     GRAAAKRSWLLHWSHRPESTQSPFWTRWIGWVMRRPWASALGASVLLLTLAAPALSMT
                     LSNSMQRQFGPEHEMRGGVNAATRALGPGALGPVRVLVTFPDGNATDPSNMQVADAVG
                     VRMREAPNIVTVQPPALADDDHSALLSAVLAVDPEDMAARDTIDWMRERLPQVPGAED
                     ARIDVGGPTALIKDFDDRVEQTQPWVFLFVSAIAFVMLLISIRSVFLALKGVLMTVLS
                     VAAAYGSIVMVFQWGWLKDLGFQPISSLDSSVPPLVLAITFGLSMDYEIFLLTRIRER
                     FLQTNNTRDAVAWGVSTSARTITSAALIMIAVYIGFAFAGMPLVAEIGVACAVAIAVD
                     ATVVRLVLVPALMAMFDQWNWWLPRWLDRILPSVDFERPLPKVDIGDVVVIPDDISSL
                     VVPNSDMRMVVKSAAKLKDLAPDAICVADPLAFSGCGRRDEEPSEAEPVVARNGHTDV
                     TTVLRPSSSARDAPTLRHRSSRKKMPWAGRGVHPVTMWRGRLSVALDALKVDSQTDAP
                     VARRSPVETTNVQLPTGDRLQIPTGAETLRMKSYLLMCRNSTRDFAEFAELVETMDTA
                     TAALVLGGMDKYYCGQHPRRQWVATQLVRRLADPQPSDEGTDPEVDWAEVKQRCLSVA
                     VAMLEEAR"
     gene            3337..3813
                     /locus_tag="VF874_22330"
     CDS             3337..3813
                     /locus_tag="VF874_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX9178698.1"
                     /translation="MAAAAQPSPLAAYEKKPVEFWPTAAIRLAVDSGDLAVWQRIVVA
                     LKRDPYGRTARQVEEVLDTASSHGIAKALTEVLARSRDQLEADERAEVARHIHALIDR
                     SGLSGREFASRIGVSCEDLASYLAGDVSPSAAQIVRMRRLSDRFAKMKAHRSTRSG"
     gene            3867..4295
                     /locus_tag="VF874_22335"
     CDS             3867..4295
                     /locus_tag="VF874_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9178699.1"
                     /translation="MWTEPDDDAVRDLLQTTRTIAVIGVSPNPLRPSNGVYRYLSSRT
                     DYELYPVNPTIDELDGRPVYADLSALPVVPDMVDVFRRRDQLRPVLDAAIAVGAKSIW
                     LQQGLWDEQLARDAEAAGMRVVMGRCLEVDHARLIATERG"
     gene            complement(4273..4971)
                     /locus_tag="VF874_22340"
     CDS             complement(4273..4971)
                     /locus_tag="VF874_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178700.1"
                     /translation="MRNAWRVFAFDIAAPLAAIAALLTIGYALDWRVWWVAVCSVLCT
                     LIVEGMIANFVLFRRDSVTVGTDDDGPGLRLAVVGLSTAALVAAVLVTYTQWTESDRD
                     LARNMDDVVQIAVKFAESTMTYSPAEPTTAIDRAAAMMEPEQAEKFKASYGTEAKNQA
                     AQNISAQAQTISAGVNAVSPDSAVVVVVVGATRQQPGKPPERAVVGLQVVLSHESGQW
                     RVFDLIPISPAPSQ"
     gene            complement(4968..5573)
                     /locus_tag="VF874_22345"
     CDS             complement(4968..5573)
                     /locus_tag="VF874_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178701.1"
                     /translation="MPEETDSPENEDAQAADDRRPVFSGLGIASAVLGLLCVAAIVLT
                     SIIWSTHRDERDELDHQSRAMQVALNWAGVLINMNKDNIEASVQKLHEGTVGTLNTDL
                     DQVIEPLTNLVKTLQLRTTGQVNAVAIESVHRDPNREPGSAPPDETVGGLASRTDTVL
                     IIATLTAKNAGGKRPPSSLNLRIGVSDVDGQLLVSGLDLLR"
     gene            5635..7593
                     /locus_tag="VF874_22350"
     CDS             5635..7593
                     /locus_tag="VF874_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX9178702.1"
                     /translation="MNSGIDLAYVDSAVRPQDDLFGHVNGCWLADYEIPADRATDGAF
                     RTLFDRAEEQVRDLITQAASNPAPHTDEQRIGDLYASFLDEDTVARVGLAPLHEELTL
                     IDEAADAATLAEVMGRLQHTGAGGGAGVYVDTDSKDSTRYLLHVNQSGLGLPDESYYR
                     DARHAEILAAYPRHVAAMFALVYGGDHADTAARIVALESKLAAAHWDVVKRRDADLTY
                     NLRAFSDLPDEAPGFDWSGWVTALGSSPDTAAELVVRQPDYLTAFAALWAGEDIADWK
                     AWLRWRVIHARAFLLTDDLVAEDFAFYGRTLSGTEQIPDRWKRGVSVVESLMGDAVGR
                     LYVERHFPPEAKARMDVLVDNLLEAYRVSIESLDWMTPQTRQRALAKLDKFTPKIGYP
                     NKWRDYSKFDVDRGDLYGNFRRGMAVTWDRELAKLGMPVDRNEWFMTPQTVNAYYNPG
                     MNEIVFPAAILQPLFFDADADDAANYGGIGAVIGHEIGHGFDDQGAKYDGDGNLVDWW
                     TDEDRTEFGARTKALIEQYDGFTPRALENGQHVNGAFTVGENIGDLGGLSIALLAYQL
                     SLGGNRASVIDGLTGLQRVFFGWAQVWRTKSRDADAIRRLAIDPHSPPEFRCNGVIRN
                     IDAFYDAFDVTESDALYLEPAHRVRIWN"
     gene            complement(7609..8649)
                     /locus_tag="VF874_22355"
     CDS             complement(7609..8649)
                     /locus_tag="VF874_22355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9178703.1"
                     /translation="MFCAIGVTAGLVFAAPLALAEPDPALPAPPPEPVDAMPVDPMAV
                     PPPVDPVAPPPPADPLAAPPPADPLAPPPPADPAAVPPLAPPPADPLAAPSVIPEGTA
                     AGQNPTPFTGTPPFRPPTFNPLNGSMVGVGKPIIINFAVPIADRGLAEQAIHISSTPP
                     VPGKFYWMSDTQVRWRPLSFWPAHTTVNIDAAGTKSSFRTGDSLIATVDNATHQMTIT
                     RNGKVEQTFPVSMGMPGHDTANGTYYVLEKFPDIVMDSATYGVPNTSAQGYKVHVQLA
                     VRFDNSGNFVHSAPWSVSDQGKRNVSHGCINISPSNAKWFFDNFGSGDPIVVKNSVGT
                     YTKNDGAQDWQI"
     gene            complement(8848..9135)
                     /locus_tag="VF874_22360"
     CDS             complement(8848..9135)
                     /locus_tag="VF874_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX9178704.1"
                     /translation="MAAQGYSDRKDDYAKRLRRIEGQVRGIAKMIDDDKYCIDVLTQI
                     SAVNSALQAVALGLLDEHLNHCVTNAVAAGGEEADAKLAEASAAIARLVRS"
     gene            complement(9140..9730)
                     /locus_tag="VF874_22365"
     CDS             complement(9140..9730)
                     /locus_tag="VF874_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX9178705.1"
                     /translation="MRIAAALAALLAVGMLTSCGYSSDQHDTRTGAQQTTEQADHNAD
                     DITFASNMVPHHEQAVVIAQLVPTNTTNQQIIDLANRIIGSQVPEIQAFRAFLMQWQD
                     AEGNDASPGHDGAGHGAAMPGMVDQATIDKLRSLRDAEFDQLWLTSMIDHHRGAIAMA
                     QDEIAHGKNPDVIYLAKSIITAQQAEIDQMKQMLGG"
     gene            9822..11582
                     /gene="ilvD"
                     /locus_tag="VF874_22370"
     CDS             9822..11582
                     /gene="ilvD"
                     /locus_tag="VF874_22370"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017680816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9178706.1"
                     /translation="MPDALRASGSSTPDALRASGSSTPEVDIKPRSRDVTDGLEKAAA
                     RGMLRAVGMGDDDFAKPQIGVGSSWNEITPCNLSLDRLAKAAKDGVFAAGGYPMEFGT
                     ISVSDGISMGHEGMHFSLVSREVIADSVETVMQAERLDGSVLLAGCDKSLPGMLMAAA
                     RLDLASVFLYAGSILPGRAKLSDGTERDVTIIDAFEAVGACSRGLMPREDVDAIERAI
                     CPGEGACGGMFTANTMASAAEAMGMSIPGSAAPPATDRRRDGFARRSGQAVVELLRRG
                     ITARDIMTKEALENAIAVVMAFGGSTNAVLHLLAIAYEANVKLTLADFTRIGAKVPHL
                     ADVKPFGKHVMFDVDRIGGVPVVMKALLDAGLLHGDCLTVTGETVADNLRHIAPPDPD
                     GKVLRALDNPLHPTGGITILHGSLAPEGAVVKSAGFDSDVFEGTARVFERERAALDAL
                     EDGTIQAGDVVVIRYEGPKGGPGMREMLAITGAIKGAGLGKDVLLITDGRFSGGTTGL
                     CVGHIAPEAVDAGPIAFLRDGDRIRLDVANATLDVLVDPAEFASRQEGFTPPPPRYTT
                     GVLAKYVKLVGSAAVGAVCT"
     gene            complement(11585..12385)
                     /locus_tag="VF874_22375"
     CDS             complement(11585..12385)
                     /locus_tag="VF874_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178707.1"
                     /translation="MAYLGYRDGDADTPFGKFFRPEMAPLPRHVVEALEHGPQAGPTL
                     LAFEAATTLDDEGYQQTENGYGLLDHGGVQVSVRTDMPGVTPAMWSWWFGWHGSDSRR
                     YKLWHPRAHVSAAWQDGSDDGRRGSARYVGRTSVISEYIGSSMLRAAIQFVTPSTFGL
                     PAADDSVVPVCARIGSSDVPVDAGWLIHHIRRTEGGSEMRSRFWLGGPYIAVRSAPAV
                     ANRVARPVVSRIIGATEDYARNLMVHCAQEMSHLAGFLPALYEQFGDE"
     gene            complement(12387..>13025)
                     /locus_tag="VF874_22380"
     CDS             complement(12387..>13025)
                     /locus_tag="VF874_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9178708.1"
                     /translation="RVELQIVASRSVPNVGIDDPAIVFASPAFADSEQEAEKALALLG
                     TCPAIDKAIVKIPYAPTDLPAWYDGVMSNYLADHRYSADNMWTSAAAEELLPGIRRII
                     ETIPPHPSHFLWLNWGPSPSRQDMAYSLEDEIYLACYGGWMDAADDDKYADWARSNMA
                     AMADLASGIQLADENLGERPARFATDEAMSRLDQVRATYDPDGRFYSWMGRI"
BASE COUNT         2079 a         4330 c         4409 g         2207 t
ORIGIN      
        1 acaggcaggc ggcgaacacg ccgtagaggc cgcggcgcac ggctgaagtc gttttcatgg
       61 agaactccta acgatttgcg gacatttcgg tggcaccgcg catcgctgcg acgctcgcgt
      121 gtggtcctcg gccagcaaac ggccgatcca catggtgcta tccgggcccg catgatcgcc
      181 aatcggagta gcgaacatcc aggtcagcgc gcctctccgg gcgcggcggc accaccgcgt
      241 gccaacccgt gcagccctcg aagggcagat cagcgcacca gatggcggac agcttcctgt
      301 tgtaggctca cgacgccggg agaaagcggg gacagccatc cagcagttca tgatgcgctt
      361 gagcagcaac ctgcgccgct accgatgggc ggtgttcacc gcctggctgc tcgtccttct
      421 tcccgccgtg ctgatcgggt tgcacgagtc aaataacctc accggcggcg ggttcgaggt
      481 cgccggttcg cagtcgctcc aggtgcagga gcagctcgaa agacactacc ctgaccaggg
      541 cgcatccccg ctggcgctgg tcgcggcacc gcgccccggg gcgaccgtcg acgacatgac
      601 cgccgcggtg gatctgctcg aacagaaagt tgccgaggtg cccagcgtgt cgctggcgcc
      661 caacccgccg gcacagccgc cgccgggccc agagcgcccg tacgtcgtct cgctgaaact
      721 gggcttcaac aactccggtg ccgtcgacgt tgccaagcag ttgcgcagca aagtcggaat
      781 caacggcgac aaaccgggac aactggacaa cggcaaagtc gacctgtacg tgatcggtca
      841 gggcgcgctc ggtgcggcgg cacaggagaa aaccagacac gacatcggcg aggccgagca
      901 gtggggcctt ccgatcatcc tgattgtcct gctcgcggtg ttcggctcac tggcggccgc
      961 ggcgatgccg ctgatcctgg gcgttctcac ggtcgtcgtg agcatgggcg tcgtcttcct
     1021 gctgtcgatg ttcaccacga tgtcggtatt cgtgacgccg acggtgtcga tgttcggaat
     1081 cgcactggcc gtggactatt cgctgttcat cctgatgcgc taccgcgagg agctgcgcgc
     1141 cggccgggac gcgaaacagg ccaccgacgc tgcgatggcc acctccggcc ttgccgtggt
     1201 gctgtcgggc ctgaccgtga tcgcatcctt ggcgggcatc tacctgatca acaccccggt
     1261 cttgcgctcg atggccaccg gcgcggtggt gaccgtcgcc gtggcagtgc tcacctcggt
     1321 cacgctgatc ccggcgatcc tcgcgacgtt cgggcgggcc gccgccaagc ggtcgtggct
     1381 gctgcactgg tcgcaccggc cggaatccac ccagtcgccg ttctggacgc gctggatcgg
     1441 gtgggtgatg cgccgtccgt gggcgtctgc gctgggagcg tccgtgctgc tgttgacgct
     1501 ggccgcaccc gcgctgtcga tgacgctgtc gaacagcatg cagcgccagt tcggacccga
     1561 gcacgagatg cgcggcggag tcaacgcggc cacgcgggcg ctggggccgg gcgcgctcgg
     1621 cccggtccga gtgctggtga cattccccga cggcaacgcg acggatccgt cgaacatgca
     1681 ggtcgccgat gccgtgggtg ttcggatgcg cgaggcgccg aacatcgtga ccgtgcagcc
     1741 cccggcattg gccgacgacg accacagcgc gctgctgtcg gccgtgctcg cggtggatcc
     1801 cgaggacatg gccgcccgcg acacgatcga ctggatgcgt gagcgccttc cccaggtgcc
     1861 cggcgccgag gacgcccgca tcgacgtcgg cggaccgacc gcgctgatca aggacttcga
     1921 cgaccgggtg gagcagaccc agccgtgggt gttcctgttc gtgtcggcga tcgcattcgt
     1981 gatgctgctg atctcgatcc ggtcggtgtt cctggcgctc aagggcgtgc tgatgaccgt
     2041 gctctcggtc gccgccgcct acggcagcat cgtgatggtg ttccagtggg gttggctcaa
     2101 agatcttggt ttccaaccta tttcgtctct cgatagctcg gttccacctc tggtgctggc
     2161 gatcaccttc gggctgtcga tggactacga gatcttcttg ctgacgcgga tcagggaacg
     2221 tttcctgcag accaacaaca cccgtgacgc ggtggcgtgg ggcgtgagca ccagcgcgcg
     2281 caccatcacc agtgcggcgc tgatcatgat cgcggtgtac atcgggttcg cgttcgccgg
     2341 catgcccctg gtggcggaga tcggggtcgc atgcgcggtg gcgatcgcgg tcgacgccac
     2401 ggtggtgcga ctggtgctgg tgcccgcgtt gatggcgatg ttcgaccagt ggaactggtg
     2461 gctgccgcgg tggctggacc ggatcttgcc gtcggtcgac ttcgaacggc cgctgcccaa
     2521 ggtcgacatc ggcgacgtcg tcgtcatccc ggacgacatt tcgtcgctgg tggtgcccaa
     2581 ctccgacatg cggatggtgg tcaagtcggc agccaagctc aaagaccttg cgcccgacgc
     2641 gatttgcgtg gcggacccgt tggcgttcag tggctgcggc cgacgggacg aagaaccgtc
     2701 ggaagccgag cccgtggtgg cgaggaacgg ccacactgac gtcacgaccg ttctccgccc
     2761 gagttcatcg gcgcgagatg ctccgacgtt gcgccaccgc agcagccgca agaagatgcc
     2821 gtgggccggt cgcggcgtac acccggtcac gatgtggcgg ggcaggcttt ccgtggcact
     2881 cgacgcgttg aaggtcgact cacagaccga tgcccccgtc gcacggcgca gcccggtgga
     2941 aaccaccaac gtccagttgc cgacgggtga ccgtctgcaa atccccacgg gcgcagagac
     3001 tttgcggatg aagagctatc tgttgatgtg ccgcaacagt acccgcgact tcgcggagtt
     3061 tgctgagctg gtcgagacca tggacaccgc cacagctgcg ttagtgctgg gcgggatgga
     3121 taagtattac tgtggtcaac atcccaggag acaatgggtt gccactcaac tcgtgcgcag
     3181 gctggccgac ccccagccgt cggacgaggg gacggacccc gaagtcgatt gggcggaagt
     3241 caaacagcga tgcctttcag tggccgtggc gatgctggag gaggcgaggt gacgttggcc
     3301 gtggaggcgc atgacgacgc ggcgcccacg gttcgcttgg ccgcagcagc gcaaccgtcg
     3361 ccgctggccg cgtacgagaa gaagcccgtg gagttctggc ccaccgcggc catccggctg
     3421 gcggtcgaca gcggcgatct ggcggtgtgg cagcggatcg tcgtggcgct caaacgcgat
     3481 ccgtacggcc gaaccgcgcg gcaagtcgag gaggtgctgg atacggcctc gtcgcacggc
     3541 atcgccaagg cactcaccga ggtactggca cgcagccgcg accagctgga ggccgacgag
     3601 cgcgcggagg tcgctcggca tatccacgcc ctgatcgacc gctcgggtct gtccggccgc
     3661 gagttcgcgt cgcgcatcgg ggtgtcctgc gaagacctcg cctcatatct cgccggcgac
     3721 gtcagcccgt cagcggcgca gatagttcgg atgcgtcggc tgtctgaccg gttcgcgaag
     3781 atgaaggcgc accgctcgac ccgctccggg tgagtggccc ccattcgctc ggcggggctc
     3841 tgacccgccg gccatacgat ggcggcatgt ggactgagcc agacgacgat gcggtgcgcg
     3901 acctgctgca gaccacccgc acgatcgcgg tgatcggcgt gtcgccgaac ccgctacggc
     3961 ccagcaacgg cgtctatcgg tacctgagtt caaggaccga ctacgagctg tatccggtca
     4021 acccgacgat cgacgaactc gacggccgcc ccgtgtacgc ggatctgtcc gcgctgccgg
     4081 tggtgccgga catggtggac gtgtttcgcc gccgggatca gttgcggccc gtgctggatg
     4141 cggcaatcgc ggtcggcgcg aagagcatct ggctgcagca gggtttgtgg gacgagcagc
     4201 tggcgcgcga cgcagaggcg gccggcatgc gggtagtgat gggccgctgc ctggaggtcg
     4261 accacgcgcg gctcattgcg acggagcggg gctgatcggg atcaggtcga aaacccgcca
     4321 ctggccgctc tcatgggaca acacgacctg aagcccgacc acggcccgct ccggcggttt
     4381 gccaggttgc tgccgcgtcg caccgaccac taccacgacg acagccgagt ctggtgagac
     4441 ggcgttcacc cccgccgaga tcgtctgggc ttgcgccgaa atattctgcg ctgcttggtt
     4501 tttcgcctcg gtgccgtagc tggccttgaa cttctccgcc tgttcgggct ccatcatggc
     4561 tgctgcgcga tctatcgccg tggtcggttc tgcgggagag tacgtcatcg tcgactcggc
     4621 gaacttgacc gcgatttgca ccacgtcatc catgttcctg gccaggtcac ggtcggactc
     4681 ggtccactgc gtgtaggtga cgagcaccgc ggccaccagc gccgcggtgc tcaacccgac
     4741 caccgccagt cgcagcccgg gtccgtcgtc atcggtgccg accgtgacgc tgtcgcggcg
     4801 gaacagcacg aagttggcga tcatgccctc gacgatcagc gtgcacagca ccgaacacac
     4861 cgccacccac cacacgcgcc agtccagcgc gtagccgatc gtcagcaggg ccgcgatcgc
     4921 ggccagcggc gcggcgatgt cgaacgcgaa aacccgccag gcgtttctca ccgcagcaga
     4981 tccaggccgg agacgagcag ctggccgtcg acgtcggaca cgccgatccg gaggttcaac
     5041 gaggatggcg gccgcttccc gcctgcgttc ttggctgtca gcgtcgcgat gatcagcacg
     5101 gtgtcggtgc gcgatgccag tccgccaact gtttcgtcag gtggcgcgct accgggttcg
     5161 cgattcgggt cgcgatgcac cgattcgatc gcgaccgcgt tgacctgccc ggtggtgcgc
     5221 agttgcagcg tcttcaccag gttggtgagc ggctcgatca cctggtccag gtcggtgttc
     5281 agcgtgccga ccgtgccctc gtgcagcttc tgaacactgg cctcgatgtt gtccttgttc
     5341 atgttgatca gcacaccggc ccagttcagc gcgacctgca tggcgcggga ctggtggtcg
     5401 agttcgtctc gctcgtcgcg gtgggtcgac cagatgatcg atgtcaggac gatcgcggcg
     5461 acgcagagca agccgagcac ggccgaggca atgcccaagc ccgagaacac cgggcggcgg
     5521 tcgtccgctg cctgtgcgtc ctcgttctca ggagagtcgg tctcctctgg cacgaaggcg
     5581 agagtactca tcagccgacg gcccgggcat cgggccttct ggaaggatgg tccggtgaac
     5641 tccggtatcg acctcgccta cgtggattcg gcggtccgcc cgcaagacga cttgttcggc
     5701 catgtcaacg gctgctggct ggcggactac gaaatccccg ccgaccgcgc caccgacggc
     5761 gcgtttcgca cgctgttcga ccgggccgag gagcaggtgc gtgacctgat cacccaagcc
     5821 gcatcgaacc ctgcgccgca caccgacgag cagcgcatcg gcgacctgta tgcgagcttt
     5881 ctcgacgaag acaccgtcgc gcgcgtcggc ctggcgccgc tgcacgagga gctgacgctg
     5941 atcgacgagg cggccgacgc ggcgacgctg gccgaggtga tgggccgcct gcagcacacc
     6001 ggggcgggcg gcggcgccgg cgtgtacgtc gacaccgact ccaaggactc gacgcgctac
     6061 ctgctgcatg tgaaccagtc cggcctcggc ctgcccgacg agtcgtacta tcgcgacgcg
     6121 cgccacgccg agatcctggc cgcgtatcca cgccacgtcg cggcaatgtt cgctctggtg
     6181 tacggcggcg accatgccga caccgccgcg cggatcgtcg cgctggagtc gaaactcgct
     6241 gccgcgcact gggatgtggt gaagcggcgc gacgcggacc tcacctacaa cctgcgggcg
     6301 ttctccgatc tgccggacga ggcgccgggc ttcgactggt ccggatgggt gacggcgctg
     6361 ggcagttcac ccgacacggc cgccgaactt gtggtgcgcc aaccggatta cctcaccgcg
     6421 ttcgccgcgc tgtgggctgg cgaggacatc gcggactgga aggcttggct gcgctggcgg
     6481 gtgatccacg ctcgcgcgtt cttgctcacc gacgatctgg tcgccgagga cttcgcgttc
     6541 tacggtcgca cgctgagcgg taccgagcag atccctgacc gctggaagcg cggcgtctct
     6601 gttgtcgaga gcctgatggg tgacgcggtc ggcaggctct acgtcgagcg gcacttcccg
     6661 ccggaagcca aggcacgcat ggacgttctg gtggacaacc tgctcgaggc gtaccgcgtc
     6721 agcatcgagt cgctggactg gatgacgccg cagacccggc aacgggcgtt ggcgaaactg
     6781 gacaagttca ccccgaagat cggctacccg aacaagtggc gcgactattc gaagttcgac
     6841 gtcgaccgtg gtgacctgta cggcaatttc cggcgcggca tggccgtcac ctgggaccgc
     6901 gagctggcca agcttggcat gcccgtcgac cgcaacgagt ggttcatgac cccgcagacc
     6961 gtcaacgcct actacaaccc ggggatgaac gaaatcgtct tccctgcagc gattttgcag
     7021 ccgctattct tcgacgccga cgcggacgac gccgccaact acggcggtat cggcgcggtg
     7081 atcggccacg agatcgggca cggcttcgac gaccagggcg ccaagtacga cggcgacggc
     7141 aatctggtcg actggtggac cgatgaggac cgcaccgaat tcggcgcgcg cacaaaggcg
     7201 ttgatcgagc agtacgacgg attcactccg cgcgcactcg agaacggcca gcacgtgaac
     7261 ggcgctttca ccgtcgggga gaacatcggc gacctcggcg gcctgtcgat cgcgctgctg
     7321 gcctaccagc tgtcgctggg cggcaaccga gcgtcggtta tcgacggtct gaccggcctg
     7381 cagcgcgtgt tcttcggctg ggcgcaggtg tggcgcacca agtcacgcga cgccgatgcg
     7441 atccgccgcc tggcgatcga cccgcacagc ccgccggagt tccgctgcaa cggcgtcatc
     7501 cgcaacatcg acgcgttcta cgacgcgttc gacgtgacgg agtccgacgc gctctatttg
     7561 gagccagccc accgcgtcag gatctggaac tgacggcccc cgagcgcgtc agatctgcca
     7621 gtcctgcgcg ccgtcgttct tcgtgtaggt cccgacggag ttcttcacga cgatcgggtc
     7681 gccgctgccg aagttgtcga agaaccactt cgcattggac gggctgatgt tgatgcagcc
     7741 gtggctgacg ttgcgcttgc cctggtcgct caccgaccac ggtgcgctgt gcacgaagtt
     7801 gccgctgttg tcgaaccgga ccgccagttg gacgtgcacc ttgtagccct gcgccgaagt
     7861 gttcggcaca ccgtaggtcg cggagtccat cacgatgtcg gggaacttct cgagcacgta
     7921 atacgtgccg ttcgccgtgt cgtggcccgg catacccatc gacaccggga acgtctgctc
     7981 caccttcccg ttgcgggtga tcgtcatctg atgcgtggcg ttgtcgaccg tcgcgatcag
     8041 cgagtccccg gtccggaagc tcgacttggt gccggccgcg tcgatgttta cggtcgtgtg
     8101 cgccggccag aagctgagcg gccgccagcg cacctgggtg tcgctcatcc agtagaactt
     8161 gcccggcacg ggcggcgtcg acgagatgtg gatcgcctgt tcggccaaac cccggtcggc
     8221 gatcggcacg gcgaagttga tgatgatcgg cttccccacc ccgaccatgg acccgttgag
     8281 ggggttgaac gtcggcggcc ggaacggcgg cgtgccggtg aacggcgtcg ggttctgtcc
     8341 ggcggccgtg ccctcgggga tcaccgaagg cgccgccagc ggatcggcgg gtggcggggc
     8401 cagcgggggc acggctgccg gatcggcggg cggcggcggc gcgagcggat cggcaggcgg
     8461 cggcgccgct aacgggtcag ccggcggcgg gggcgcgacc ggatcaaccg gcggcggcac
     8521 ggccatcggg tccacaggca tcgcgtccac gggttcgggg ggcggcgccg gcagtgcggg
     8581 atccggctcg gccagcgcca acggcgcagc gaaaaccagc ccggcggtca cgccgattgc
     8641 gcaaaacatt gaggtcaacg tccgctttgt gccccgctgc gacgcccagc gccatgcggc
     8701 tccggctcgt ctactcatca aaccctccag acgatcgagt gcccccagtg tggcacaggg
     8761 ccagaggtcg tcatgaccag ataccggtcc ccgaccccat gacagctacc tagcccatct
     8821 ctttgaactg cggattcgtt acaccggtca cgaccggacc aggcgtgcga tcgcggccga
     8881 cgcctcggcc agcttggcgt cggcctcctc gccgcccgcc gcgacagcgt tggtgacgca
     8941 gtggttgagg tgttcgtcga gcaggccgag cgccacggct tgcagcgcgc tgttgacggc
     9001 gctgatctgg gtgaggacgt cgatgcagta cttatcgtcg tcgatcatct tcgcgatgcc
     9061 gcgaacctgg ccctcgatgc gccggagccg cttggcgtaa tcgtccttgc ggtccgaata
     9121 tccctgcgct gccacggact tatcctccca gcatctgctt catctggtcg atctcggcct
     9181 gctgcgcggt tatgatcgac ttggccaaat agatgacgtc ggggttcttg ccgtgcgcga
     9241 tttcgtcctg cgccatcgcg atcgcccctc ggtggtggtc gatcatcgag gtcagccaca
     9301 gttggtcgaa ctcggcgtcg cgcagcgacc gcagcttgtc gatcgtggcc tggtccacca
     9361 tgccgggcat cgccgcgccg tgcccggccc cgtcgtgacc gggggacgcg tcgttgccct
     9421 cggcatcctg ccactgcatc agaaacgcgc gaaacgcctg gatctccggt acctggctac
     9481 cgatgatgcg gttcgccaga tcgatgatct gttggttcgt cgtgttcgtg ggcaccagct
     9541 gcgctatcac gacggcctgc tcgtggtgcg gcaccatatt gctggcgaac gtgatgtcgt
     9601 cggcgttgtg gtcggcctgc tcggtggtct gctgcgcgcc ggtccgggtg tcgtgttgat
     9661 ccgacgagta cccgcacgac gtcagcatgc cgacagcgag cagcgccgcc aacgctgccg
     9721 ctatcctcat gcctccactc taccctggta cccctacggg gtatcaaaac caacggctga
     9781 cctctgtctt gagcccgcgc gggcgaaggc atacttgaac gatgccagat gctcttcgcg
     9841 caagcggctc atcgacgcca gatgctcttc gcgcaagcgg ctcatcgacg ccagaagtcg
     9901 acatcaagcc ccgtagccgc gacgtcaccg acggcctgga gaaagccgcc gcccgcggca
     9961 tgctgcgggc cgtcggcatg ggggacgacg acttcgccaa gccgcagatc ggcgtcgggt
    10021 cgtcgtggaa cgagatcact ccgtgcaacc tgtcgctgga ccggctcgcg aaggccgcca
    10081 aggacggggt gttcgcggcc ggcggctatc cgatggagtt cgggacgatt tcggtgtccg
    10141 acggcatctc gatgggccat gagggcatgc acttctcgct ggtgtcccgc gaggtgatcg
    10201 ccgacagcgt cgaaacggtg atgcaggccg agcggctgga cggctccgtg ctgctggccg
    10261 ggtgcgacaa gtcgctgccc ggcatgctga tggccgccgc ccgcctcgac ctcgcgtcgg
    10321 tgttcctgta cgccggatcg atcctgccgg gccgggccaa gctgtccgac ggcaccgagc
    10381 gcgacgtcac gatcatcgac gcgttcgagg ccgtcggcgc ctgcagtcgc gggttgatgc
    10441 cgcgcgagga cgtcgacgcg atcgagcggg cgatctgccc cggcgaaggc gcctgcggcg
    10501 gcatgttcac cgcgaacacg atggccagtg cagccgaggc gatgggcatg tcgattcccg
    10561 gcagcgccgc accaccggcc accgaccgcc gccgcgacgg cttcgcccgc cgcagcggcc
    10621 aggcagtcgt cgaactgttg cgccgcggga tcaccgcccg cgacatcatg accaaggagg
    10681 cgttggaaaa cgcgatcgcc gtggtgatgg cgttcggtgg ttcgacgaac gcggtgttgc
    10741 acctgctcgc gatcgcctac gaggccaacg tgaagctgac gctggccgat ttcacccgca
    10801 tcggcgcgaa ggtgccgcat ctggcggacg tgaagccgtt cggcaagcac gtgatgttcg
    10861 acgtcgaccg gatcggcggt gtcccggtgg taatgaaagc actgctggac gccggtctgc
    10921 tgcatggtga ttgccttacc gtcaccggtg agacggtggc cgacaacctc aggcacatcg
    10981 cgccgccgga ccccgacggg aaggtgctgc gcgcgctgga caacccgctt catcccaccg
    11041 gcggcatcac gatcctgcac ggctcgctgg cccccgaggg cgccgttgtc aagtctgccg
    11101 ggttcgactc cgacgtattc gaaggcaccg caagggtttt cgagcgcgag cgggctgcgc
    11161 tagacgcgct ggaggacggc acgatccagg ccggcgacgt cgtcgtcatc cgctacgaag
    11221 gccccaaggg cggacccggc atgcgcgaga tgctcgcgat caccggcgcg atcaagggcg
    11281 cagggctcgg caaggacgtg ctgctgatca ccgacggccg gttctccggc ggcaccaccg
    11341 ggctgtgcgt cgggcacatc gcacccgagg ccgtcgacgc cgggcccatc gctttccttc
    11401 gcgacggcga ccggattcgc ctcgacgtgg ccaacgcgac gctcgacgtg ctcgtcgacc
    11461 cggccgaatt cgcctcgcgc caagagggtt tcacaccgcc gccgccccgc tacaccaccg
    11521 gcgtgctggc caagtacgtc aagctggtcg gctcggcagc cgtcggcgcc gtctgcacgt
    11581 agggctactc gtcgccgaac tgctcgtaga gcgcgggcag gaaacccgcg aggtggctca
    11641 tctcctgcgc gcagtgcacc atcaggttgc gcgcgtaatc ctctgtcgca ccgatgatgc
    11701 gggacacgac cggcctggcg acacggttgg ccactgcggg cgcgctgcgc acggcgatgt
    11761 aaggtccgcc cagccagaac cgggatcgca tctcactgcc gccctccgtc cgccgaatgt
    11821 ggtggatcaa ccacccggcg tccaccggga cgtcgctgga gccgatccgg gcacagaccg
    11881 ggaccacgct gtcgtcggcg gcgggcaggc cgaacgtcga aggcgtgacg aattgaattg
    11941 ccgcgcgcag catcgacgag ccgatgtact cgctgatcac cgacgtgcgc ccgacgtagc
    12001 gggcggagcc gcggcgcccg tcgtcggatc cgtcctgcca cgccgccgac acgtgcgccc
    12061 tggggtgcca cagcttgtag cgacgtgagt cggagccgtg ccagccgaac caccaggacc
    12121 acatcgccgg cgtaaccccg ggcatgtcgg tgcgcaccga cacctgcacg ccgccgtggt
    12181 cgagcagccc gtagccgttc tcggtctgct gatagccctc gtcgtcaagc gtcgtcgcgg
    12241 cctcgaacgc cagcagcgtc ggcccggctt gcggaccgtg ctccagcgcc tcgacgacgt
    12301 gccgcggcag cggcgccatc tccggccgga agaacttgcc gaacggggta tccgcgtcgc
    12361 cgtcgcggta accgaggtaa gccatgtcag atccgtccca tccagctgta gaagcgtcca
    12421 tcggggtcat aggtggcgcg gacctggtcg agccgcgaca tcgcctcgtc ggtggcgaac
    12481 ctcgccggac gctcgcccag gttctcgtcc gcgagttgga tgccgctggc caggtcggcc
    12541 atggccgcca tgttggaccg cgcccagtcg gcgtacttgt cgtcgtcggc ggcgtccatc
    12601 cagccgccgt agcacgccag gtagatctcg tcttcgaggc tgtaggccat gtcctggcgg
    12661 gacggcgacg ggccccagtt cagccacagg aagtgcgacg gatgcggtgg gatggtctcg
    12721 atgatgcggc gaatccccgg cagcaactcc tccgcggcgg ccgacgtcca catgttgtcg
    12781 gcggagtatc ggtggtcggc cagatagttg ctcatcacgc cgtcatacca ggccggcaaa
    12841 tcggttgggg cataggggat tttgacgatc gccttatcga tggccgggca ggtgccgagt
    12901 aacgccagcg ccttctcggc ttcctgctca gagtccgcga atgctggtga cgcgaaaacg
    12961 attgcgggat cgtcgatccc gacgttcggc acgctgcgtg acgcgacgat ctgcagctcc
    13021 acccg
//
LOCUS       DASWBF010000603         9903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_1117428,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000603 DASWBF010000000
VERSION     DASWBF010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9903
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117428"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VF874_22385"
     CDS             complement(<1..491)
                     /locus_tag="VF874_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178709.1"
                     /translation="MRRRPTFVAAAIVLVCVAGCSRTVGGSAVVASESRDLATIPLPE
                     LMLEPSAFPPEYPATSLDAGAAQSALALVDGVPPGGRVTPPECQPAAPRQIAAMEGVH
                     GDITLRVMLARGVGPLSARRDQVAWCSTFSVTTDTNGSWDVSAQLLPPPVLDIDDAFA
                     VDQT"
     gene            611..2014
                     /gene="sthA"
                     /locus_tag="VF874_22390"
     CDS             611..2014
                     /gene="sthA"
                     /locus_tag="VF874_22390"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEX9178710.1"
                     /translation="MEYDLVVIGSGPGGQKAAIAAAKLGKSVAVVERGRMLGGVCVNT
                     GTIPSKTLREAVLYLTGMSQRELYGASYRVKDKITPADLLARTTHVINKEQDVVRSQL
                     MRNHIELYSGHGRFIDEHTVAVEDAIRGDRITVSGRYIVIATGTKPARPAGVEFDEDR
                     VLDSDGILDLKMLPASMVVVGAGVIGIEYASMFAALGTKVTVVEKRDSMLDFCDPEIV
                     EALRFHLRDLAVTFRFGEEVTAVDVGSAGTVTTLASGKQIPAETVMYSAGRQGYTDHL
                     DLGNAGLESDSRGRIYVDDQFQTKVDHIYAVGDVIGFPALAATSMEQGRLAAYHAFGE
                     PSAGMTALQPIGIYSIPEISYVGATEVQLTKDSVPYEVGVSRYRELARGQIAGDSYGM
                     LKLLVSTDDLKLLGVHIFGTSATELVHTGQAVMGCGGTIEYLVDAVFNYPTFSEAYKV
                     AALDVMNKMRALNQFRA"
     gene            complement(2015..2773)
                     /locus_tag="VF874_22395"
     CDS             complement(2015..2773)
                     /locus_tag="VF874_22395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX9178711.1"
                     /translation="MRFRHALVILLLSAAFWPLGCTPPHPAATASPPASAAPVDADPV
                     VGAVFLGASDMHTCTASVLHSSIGNLILTAAHCLATGYPTTFATGFSGHAEPAWTVDA
                     IYLDPRWVADQDPDADYAIARVTRPDGGSVESATGPGLTLGTAPAAGTAVTVTAYPLG
                     VGGVPIGCRAVTETVTAGFPSLQCPGLADGTSGAPWRTDSTVVGLIGGLDGGGCDENV
                     SYSPPFDAPLADLLHRAEAGGPADTGPEVFENDC"
     gene            2949..3923
                     /locus_tag="VF874_22400"
     CDS             2949..3923
                     /locus_tag="VF874_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9178712.1"
                     /translation="MADHHDEYRPEHSGMYELEFPAPQLSSPDGRGPVMIHALEGFSD
                     AGHAIRLSSKYLRDALDTELVASFAIDELLDYRSRRPLMTFKTDHFTKYDEPELSLYA
                     LHDTVGTPFLLLAGMEPDLKWERFVTAVRLLAERLAVRQTIGLGTIPMAVPHTRPVTM
                     TAHANNKELIAHHQPWVGEVQVPGSASNLLEYRMAQHGHEVVGFTVHVPHYLAQTDFP
                     AAAEALLAEVGKIGSLQIPLAALAEAAAEIRAKIDEQVNASPEVVQVVEALERQYDAF
                     VAAQENRSLLARGEDLPSGEELAGEFQRFLAQQAGDFSDGFRDGDDKK"
     gene            3963..4982
                     /locus_tag="VF874_22405"
     CDS             3963..4982
                     /locus_tag="VF874_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9178713.1"
                     /translation="MARKANLRPVRDVRSTLHFRTIHGYRRAFRVAGEGPAILLIHGI
                     GDNSATWEEVQAKLAQRFTVIAPDLLGHGKSDKPRADYSVAAYANGMRDLLAVLDIDR
                     VTVVGHSLGGGVTMQFAYQYPQLVERIVLVGTGGVTKDVHFALRLASMPMGSEALALL
                     RMPGALPALQILGRVVGTVLGSTRFGRDLPDVLRVLAELPEPTASSAFSRTLRAVVDW
                     RGQVVTMLDRCYLTQSVPVQLIWGEDDSVIPVSHARMAHAAMPGSQLEIFEKSGHFPF
                     HDDPDRFVEIVERFIDSTEPSNHDPDVLRALLRTGISDETISGSVDTRVAVLDAMSSD
                     ERSAT"
     gene            5091..5252
                     /locus_tag="VF874_22410"
     CDS             5091..5252
                     /locus_tag="VF874_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178714.1"
                     /translation="MTSKGLRVDGIKREQDARRAVHALGMSSVISPYSWQVVDNHGDR
                     FVVELRRAS"
     gene            5283..5969
                     /locus_tag="VF874_22415"
     CDS             5283..5969
                     /locus_tag="VF874_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX9178715.1"
                     /translation="MGTDVSVVRVFTDSEGNFGNPLGVVDASTVTPADRQALATQLGY
                     SETIFVDMPTSGATTAHARIFTPTTELPFAGHPTVGLSWWLKSRGIAIHTLQVPAGVV
                     AVSYEDDVTTVRARADWAPEFALHELASPEEVVATDPDDYPDDVAHYIWAWIDEPGGH
                     LRSRMFATDLGVAEDEATGAAAVRITDILSRDLTITQGKGSVIHTWWSPEGWVKVGGR
                     VVDDGTVRAD"
     gene            complement(5974..6438)
                     /gene="nrdR"
                     /locus_tag="VF874_22420"
     CDS             complement(5974..6438)
                     /gene="nrdR"
                     /locus_tag="VF874_22420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX9178716.1"
                     /translation="MHCPFCRHPDSRVVDSRETDEGQAIRRRRSCPECGRRFTTVETA
                     VLAVVKRSGVTEPFSREKVIRGVRRACQGREVDDDALNLLAQQVEDTVRATGSPEVPS
                     QEVGLAILGPLRELDEVAYLRFASVYRSFTSAADFEREIEALRAHRNVTASK"
     gene            complement(6525..7025)
                     /locus_tag="VF874_22425"
     CDS             complement(6525..7025)
                     /locus_tag="VF874_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9178717.1"
                     /translation="MIDYIRTQERKMMAEGTRMTIIDTWELPTPRPSYRSTERTRPRR
                     RAAVRPGGGAVAYRGTGVRTSRALHRPRPVSVTMTIVLAGIAALITVWLIALAQARTA
                     DTAATSVPEQLAVVQVQPGENLQRLAARLAPDAPVAGVVERIRELNELDSAALDAGQT
                     LIAPVG"
     gene            7158..7838
                     /gene="lexA"
                     /locus_tag="VF874_22430"
     CDS             7158..7838
                     /gene="lexA"
                     /locus_tag="VF874_22430"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX9178718.1"
                     /translation="MSDSSGTPDSTPRAGLTQRQRTILDVIRASVTTRGYPPSIREIG
                     DAVGLTSTSSVAHQLRTLERKGYLRRDPNRPRAVDVRGADEAAGPTVTTDVAGSDTLP
                     EPTFVPVLGRIAAGGPILAEEAVEDVFPLPRELVGEGSLFLLKVVGESMVDAAICDGD
                     WVVVRQQNTADNGDIVAAMIDGEATVKTFKRTGNQVWLMPHNPAFDPIPGNEAAILGR
                     VVTVIRKI"
     gene            7873..8127
                     /locus_tag="VF874_22435"
     CDS             7873..8127
                     /locus_tag="VF874_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178719.1"
                     /translation="MGPFGRRAVRLISVAAVGAVVVIAPVIGAPGTETHVAICPRGMV
                     DNPAGYGCVPYAPGVGAPSQEVLTRCHGNYYICVWPYPVP"
     gene            complement(8203..9363)
                     /locus_tag="VF874_22440"
     CDS             complement(8203..9363)
                     /locus_tag="VF874_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9178720.1"
                     /translation="MGNAVKYERTLFEPEHELFRESYRAFLERHVAPHHEQWESDKIV
                     DRGVWLEAGKQGFLGMAVPEEYGGGGDPDFRYNTIITEETTLGRYSGIGFGLHNDVVA
                     PYLLRLANEEQKQRWLPKFCTGELITAIAMTEPGTGSDLQGIKTRAVKEGDHYVLNGS
                     KTFITNGINSDLVIVVAQTDQEKGALGFSLLVVERGMEGFERGRHLDKIGLDAQDTAE
                     LSFTDVKVPAENLLGEEGQGFVYLIQNLPQERISIAIMAAAAMEGVLEQTLQYTKERK
                     AFGKPIGSFQNTRFVLAELATETTVVRIMVDEFIRLHLDEKLTAEQAAMAKWYSTEKQ
                     VHLIDRCLQLHGGYGYMREYPIARAYLDSRVQTIYGGTTEIMKEIIGRSLGV"
     gene            9480..>9903
                     /locus_tag="VF874_22445"
     CDS             9480..>9903
                     /locus_tag="VF874_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178721.1"
                     /translation="MAETIRVQERGRIIEFSFDDLMRYHGPGSPGGVANAFKVLQRAF
                     AVLSPDEPPHRRSVMVRTAFRGPGARDGVEAVTRAVTDGRYIVDPALTRPDLGRLRES
                     FVFEVRIADRSVTLLLRDGFVTEEFIDLAQKDDRTDEDE"
BASE COUNT         1647 a         3350 c         3293 g         1613 t
ORIGIN      
        1 gtctggtcga cggcgaacgc gtcgtcgatg tcgagcaccg gcggcggcag caactgggcg
       61 ctgacatccc acgagccgtt ggtgtccgtc gtcaccgaga aggtcgagca ccaggccact
      121 tggtcgcggc gcgcgctcag cggcccgacc ccccgcgcca gcatcacccg caacgtgatg
      181 tcgccgtgca cgccttccat ggcggcgatc tgcctgggcg ccgcaggctg gcactcgggc
      241 ggcgtgacgc gaccgcccgg cggcacgccg tcgaccagcg ccagtgcgct ctgcgctgcg
      301 ccggcgtcga gtgacgtggc cggatactcg ggcggaaacg ccgacggctc gagcatcaac
      361 tccggtaacg ggatcgtcgc caagtcccgc gactcagagg ccacgaccgc ggaaccgccg
      421 accgttctgc tgcaaccggc gacgcacacc agcacgatcg ccgctgccac gaacgtcggc
      481 cgcctcctca tcgcggccat agtctcaccg acggtgccgc cgaagcgtgc gcgacacagc
      541 aacttcacac gggattgatg cagtcggggt gcgagatccg gctaaacggc gtacatctct
      601 actcatgggt atggagtacg acctcgtcgt catcgggtcc ggccccggcg gacagaaggc
      661 ggcgatcgcc gcggcgaagc tgggcaagtc cgtcgcggtg gtcgaacggg gccggatgct
      721 cggcggggtg tgcgtcaaca ccgggaccat cccgtccaag acgctgcgcg aggccgtgct
      781 ctacctcacc ggcatgagtc agcgggaact gtacggcgcc agctaccgcg tcaaggacaa
      841 gatcaccccc gccgacctgc tggcgcggac cacgcacgtc atcaacaagg agcaggacgt
      901 cgttcggtcg cagttgatgc gcaaccacat cgagctctac agcggccatg gccggttcat
      961 cgacgagcac accgtggcgg tcgaggacgc gatccgcggt gaccgcatca ccgtcagcgg
     1021 ccgctacatc gtgatcgcca ccggcaccaa gccggcgcgc ccggccggcg tcgagttcga
     1081 cgaagaccgg gtgctggact ccgacggcat tctcgacctc aagatgctgc cggcgtcgat
     1141 ggtggtggtc ggcgccggcg tgatcggcat cgagtacgcg tcgatgttcg ccgcgctggg
     1201 caccaaggtc acggtcgtcg agaaacgcga ctcgatgctg gatttctgcg accccgagat
     1261 cgtcgaggcg ctgcgctttc acctgcgcga cctggcggtc acgttccggt tcggcgagga
     1321 ggtgacggcc gtcgacgtcg gctccgcggg caccgtcacc accctggcca gcggcaagca
     1381 gatccccgcc gaaaccgtca tgtactccgc cggacggcag ggctataccg atcacctgga
     1441 cctgggcaac gccggcctgg aatccgacag ccgcggccgc atctacgtgg acgaccagtt
     1501 ccagaccaag gtcgaccaca tctacgcggt cggcgacgtg atcggcttcc cggcgctggc
     1561 cgcaacctcg atggagcagg gccggctcgc cgcctaccac gcgttcggcg aaccctccgc
     1621 aggcatgacc gcactgcagc ccatcggcat ctactcgatt cccgagattt cctacgtggg
     1681 cgccaccgag gtgcaactga ccaaggactc cgtgccgtac gaggtcgggg tgtcgcgcta
     1741 ccgcgagctc gcgcggggcc agatcgccgg cgactcgtac ggcatgctca agctgctggt
     1801 ctccaccgac gacctcaaac tgctgggtgt gcacatcttc ggcaccagcg ccaccgaact
     1861 cgtgcacacc gggcaggccg tgatgggctg cggcggcacc atcgagtacc tcgtcgacgc
     1921 ggtcttcaac tacccgacgt tctcggaggc gtacaaggtc gcggcgctcg acgtgatgaa
     1981 caagatgcgc gcgctcaacc aattccgggc gtagtcagca gtcgttctcg aagacttccg
     2041 gcccggtgtc ggcgggtccg cccgcctcgg cgcggtgcaa caggtcggcc agcggggcgt
     2101 cgaacggcgg cgagtacgag acgttctcgt cacaccctcc tccgtccagg ccgccgatca
     2161 gtccgacgac ggtcgagtcg gtgcgccacg gcgcgccgct ggtgccgtcg gccaatccgg
     2221 ggcactgcag cgacgggaac ccggcggtaa cggtttccgt gaccgcgcgg caaccgatcg
     2281 gcacgccgcc cacgcccagc gggtaggcgg tcaccgtgac agcggttccg gcggcaggcg
     2341 ccgtgccgag cgtcaggccc gggcctgtcg ccgattcgac ggagccgccg tccggccggg
     2401 ttacccgtgc aatcgcgtag tcggcgtcgg gatcctggtc ggccacccag cgcgggtcga
     2461 ggtagatcgc gtcgacggtc cacgccggct cagcatggcc gctgaagccg gtcgcgaacg
     2521 tcgtcggata ccccgtcgcc aggcagtgcg ccgccgtgag gatcaggttt ccgatgctcg
     2581 aatgcagcac tgacgcggtg cacgtgtgca tgtctgaggc gcccaggaac acggcgccga
     2641 cgaccgggtc tgcatcgacg ggcgcggccg atgccggtgg cgacgccgtg gcggctggat
     2701 gcggcggggt gcaacccagc ggccagaaag cggcgctgag cagcaggatc accaacgcgt
     2761 gccggaaacg catcacgtcg atcatgcccc aggtgagcgg ggctggctcg gcgtggaacg
     2821 catccgcgcc cctgcggcgt tgagaaatca gggatacctg cgttcgcaaa tcgcagtgat
     2881 cgagtggcgg aggtcacgtc gcccagcgac actagacgtc accggcactg cggaaggagg
     2941 cgagaaccat ggctgatcac cacgacgagt accggcccga acacagcggg atgtacgagc
     3001 tcgagttccc cgccccgcag ttgtcctccc ccgacggccg cggtccggtg atgatccacg
     3061 cactggaagg cttctccgac gccggtcacg cgatccggtt gtcgtcgaag taccttcgcg
     3121 acgcactcga caccgagctc gtcgcatcgt ttgcgatcga cgagctgctg gactaccggt
     3181 cccgtcgtcc actgatgaca ttcaagacgg atcacttcac gaagtacgac gagccggagc
     3241 tgagcctgta cgcgctgcac gacaccgtcg gcacgccgtt tctgctgctg gccggcatgg
     3301 agcccgacct gaagtgggag cggttcgtca ccgcggtgcg gctgctggcc gagcggctcg
     3361 ctgtgcggca gaccatcggc ctgggcacca tcccgatggc cgttccgcac acccggccgg
     3421 tcacgatgac cgcgcacgcc aacaacaagg agctgatcgc gcaccatcag ccgtgggtcg
     3481 gcgaggtgca ggttccgggc agtgcgtcga acctgctgga gtaccgaatg gctcagcacg
     3541 gccacgaggt cgtcggcttc accgtgcatg ttccgcacta cctggcgcag accgacttcc
     3601 ccgccgccgc tgaagcgctg ctggccgagg tcggcaagat cggttcactg cagataccgc
     3661 tggccgcgct ggccgaggcg gcggccgaga tccgggcgaa gatcgatgag caggtcaacg
     3721 ccagccccga ggtggtgcag gtggtggagg cgctggagcg ccaatacgac gcattcgtcg
     3781 ccgcccagga gaaccggtcg ctgctggccc gcggtgaaga cctgcccagt ggcgaggaac
     3841 tcgccgggga gttccaacgc ttcctggcgc agcaagcagg ggacttctcg gacgggttcc
     3901 gagacggcga cgacaagaag tgagtcgagg cgggccgccg gcccgaggcg gaggtggccc
     3961 acatggcgag gaaagccaac ctacggccgg tgcgcgacgt gcggtccacg ctgcacttcc
     4021 gcaccatcca cggctaccgc cgcgcgttcc gggtcgccgg cgaggggccc gcgatcctgc
     4081 tgatccacgg catcggcgac aactcggcga cgtgggagga agtgcaggcc aagctggcgc
     4141 agcggttcac ggtgattgca ccggacctgc tgggtcacgg aaagtccgac aagccccgcg
     4201 ccgactactc ggtggccgcc tacgccaacg gcatgcggga tctcctcgcc gtgctcgata
     4261 tcgaccgcgt caccgtcgtc ggccactcgc tgggcggcgg cgtcacgatg caattcgcct
     4321 accagtaccc gcaattggtg gaacggatcg tgctggtcgg caccggcggg gtcaccaagg
     4381 acgtgcactt cgcgttgcgg ttggcgtcga tgccgatggg cagcgaggcg ctcgcgttgt
     4441 tgcggatgcc gggtgcgttg ccggcgctgc agatcctcgg gcgcgtggtg ggcacggtgc
     4501 tcggctcgac gaggttcggg cgcgacctgc ccgacgtgct gcgggtgctg gccgagttgc
     4561 ccgagccgac ggcctcgtca gcgttctcgc ggacgctgcg ggccgtggtg gattggcgcg
     4621 gccaggtggt gacgatgctc gaccgatgtt atttgacgca atccgttccc gtgcaactga
     4681 tttgggggga agacgactcg gtgatcccgg tcagccacgc gcggatggcg cacgcggcga
     4741 tgcccggatc gcagctggag atcttcgaga aatcgggcca cttcccgttc cacgacgacc
     4801 cggaccgctt cgtcgagatc gtcgagcggt tcattgactc cacggaaccg tccaaccacg
     4861 atcccgacgt gcttcgcgcg ctgctgcgca ccgggatcag cgacgagacg atctccggct
     4921 cggtcgacac acgcgtcgcg gtgctcgacg cgatgagttc cgacgagcgc agcgcgacct
     4981 gacctgcgct ttccaagatt tctccaagga ctgccctgca gtcttctctc aacgaaccgc
     5041 ttcggcggcc cgagaagcag acagtcctgg aggaaacgat gtggagcctt atgacatcca
     5101 aaggcctgcg cgtcgacggc attaagcgcg aacaagacgc gcggcgcgcc gtccacgcgc
     5161 tcggcatgtc atctgtgatc agcccctact cgtggcaggt cgtggacaac cacggcgacc
     5221 gcttcgtggt agagctcaga agggcgagct gaccgaccca caaggcacgt agagtcgggt
     5281 tgatgggtac cgacgtgtca gtggtgcgcg tgttcaccga ctcggaagga aacttcggaa
     5341 atccgctcgg cgtggtcgac gccagcacgg tcacgcccgc cgaccggcaa gcgctcgcca
     5401 cccaattggg ttacagcgaa acgattttcg tcgacatgcc gacatccggc gccaccacgg
     5461 cccacgcgcg aatcttcacg cccaccacgg agttgccctt cgccggccac ccgaccgtcg
     5521 ggttgtcgtg gtggttgaag agccgaggga ttgcgatcca cacgttgcag gtgcccgctg
     5581 gcgtggtcgc cgtcagttac gaggatgacg tgaccaccgt gcgcgcccgc gccgactggg
     5641 cgccggagtt cgcgttgcac gagctggcct ccccggagga ggtcgtggcc accgacccgg
     5701 atgactaccc cgacgacgtc gcgcactaca tctgggcgtg gatcgacgag ccgggcgggc
     5761 atctgcggtc gcggatgttc gccaccgact tgggtgtggc ggaggacgag gccaccggcg
     5821 cggcggcggt ccgtatcacc gacatcctca gccgcgatct cacgatcacc caaggcaagg
     5881 gctccgtcat ccacacctgg tggagcccgg aaggctgggt gaaagtgggc ggtcgcgtcg
     5941 tcgacgacgg caccgtgcgc gccgactgag cgcctacttc gacgcggtga cgttgcggtg
     6001 ggcgcgcagc gcctcgatct cgcgttcgaa atcggcggcg gacgtaaacg accggtacac
     6061 cgacgcgaac cgcagatagg cgacttcgtc gagttcccgc aacggcccga ggatggccag
     6121 tccgacctcc tggctgggca cctcgggtga cccggtggcc cgcaccgtgt cctcgacctg
     6181 ttgcgcgagc aggttcaggg cgtcgtcgtc gacctcgcgt ccttgacacg cccgtcggac
     6241 gccgcggatc actttctcgc ggctgaacgg ttcggtgacg ccgctgcgct tgaccaccgc
     6301 gagcacggcg gtctcgacag tggtgaaccg gcggccacac tccggacatg agcggcgacg
     6361 ccggatcgct tggccctcat cggtttcgcg ggagtcgacg acccgggagt cgggatggcg
     6421 gcagaacgga cagtgcatga ctgctccttc gccgcggaga cactcggtcg agtcgaacgc
     6481 ctccgagact actcgcgcgt gccccgccaa cgtcgccgca ggcatcagcc gaccggggcg
     6541 atcagcgtct gcccggcatc cagcgccgcc gaatccagct cgttgagctc cctgatccgc
     6601 tcgacgacgc cggccaccgg tgcgtccggc gccaaccggg ccgcaagcct ctgcaggttc
     6661 tcaccgggct gcacctgcac caccgcgagt tgctcgggta cggacgtggc tgccgtgtcg
     6721 gcagttcgag cctgcgccaa cgcgatcagc cagacggtga tcagcgcggc gattccagcc
     6781 agaacgatcg tcatcgtgac gctgaccggc cgcggccgat ggagggcacg agacgtcctc
     6841 accccggtgc cgcgatacgc caccgcgccg ccacctgggc ggaccgcggc tctgcgtcgc
     6901 ggccgggtcc gctctgtgct gcgatacgac ggtctcggcg tcggcagttc ccaggtgtcg
     6961 atgatcgtca tgcgcgtccc ttccgccatc atctttcgct cctgtgttcg aatatagtcg
     7021 atcatgcgtt cgaagtacga acatttgatc gatgtttatc agaggccacc gacagccggc
     7081 cgcggcggag gtcacgaacg cgacacacct cgaacacatg tttgaagatg tcgccgccga
     7141 gggttacatt tggccccatg agcgacagca gcggcacacc agactccacg cccagggcgg
     7201 ggctcaccca gcgtcagcgc acgatcttgg acgtgatccg cgcgtcggtc accacaaggg
     7261 ggtatccgcc gagcattcgc gagatcggcg acgccgtcgg gctcacgtca acctcgtcgg
     7321 tggcccatca actgcgaacg ctggagcgca agggctatct gcgccgcgat cccaaccggc
     7381 cgcgtgcggt cgacgtccgc ggggccgacg aggcggctgg gcccaccgtc accaccgatg
     7441 tcgctgggtc ggacacgctg ccggaaccga cgttcgtgcc ggtgctcgga cgcatcgctg
     7501 ccggcgggcc gatcctcgcc gaagaggcgg tcgaagacgt gttcccgctc ccccgcgagc
     7561 tggtcggcga gggctcgcta ttcctgctca aggtggtggg cgagtcgatg gtcgacgccg
     7621 cgatctgcga cggcgactgg gtggtggtgc ggcagcagaa caccgccgac aacggcgaca
     7681 tcgtcgcggc gatgatcgac ggcgaggcca cggtgaagac gttcaagcgc accggcaacc
     7741 aggtgtggct gatgccccac aacccggcgt tcgatccgat cccgggcaac gaggccgcca
     7801 ttctcggccg tgttgtgacg gtgatccgca agatctagcc cgtaacccgc gtgtcgggtg
     7861 cattgtggtg gcatgggccc gttcggacgt cgggcggttc gactgatttc ggtggcagcg
     7921 gtcggtgcag tggtcgtcat cgcgccggtg atcggtgcac ccgggactga gacccacgtc
     7981 gcgatatgcc cgcggggcat ggtcgacaac cccgcgggct acggctgcgt gccgtacgcg
     8041 cccggagtcg gcgcgccgag tcaggaagtc ctcacacgct gtcacggcaa ctactacatc
     8101 tgcgtgtggc cgtacccggt tccctagttc gccgaaattt gcattccacg tgggctgttc
     8161 tcgaaaaagt ccgcggcagt tacagtttcg gcgcgaagag ctctaaacgc ctagcgagcg
     8221 accgatgatc tccttcatga tctccgtcgt accgccgtag atggtctgca cacgcgagtc
     8281 caaatacgcc cgcgcgatcg ggtattcgcg catgtagccg tagccaccgt gcagctgcag
     8341 gcaccggtcg atcaaatgca cctgcttctc ggtggagtac cacttggcca tcgcggcctg
     8401 ctctgcggtc agcttctcat cgaggtgcag gcgaatgaac tcgtcgacca tgatgcgcac
     8461 gacggtggtt tctgtcgcca actctgccag cacgaagcgg gtgttctgga agctgccgat
     8521 cggcttgcca aaagccttgc gctccttggt gtattggagg gtctgctcca gcaccccttc
     8581 catcgcagcg gccgccatga tcgcgatcga gatccgctct tgcggaaggt tctggatcag
     8641 gtaaacgaag ccctggcctt cctcgccgag caggttctcg gccggcacct ttacgtcggt
     8701 gaacgacagc tcggcggtgt cctgagcgtc gaggccgatc ttgtcgagat ggcggccgcg
     8761 ttcgaagccc tccatcccgc gctcgacgac aagcagcgag aagcccagtg cgcccttctc
     8821 ctgatcggtc tgcgcgacga cgatcaccag atcggagttg atgccgttgg tgatgaacgt
     8881 cttcgagccg ttgagcacgt agtggtcgcc ctctttgact gcacgcgtct tgatgccttg
     8941 caggtcgctg cccgtgcccg gctcggtcat cgcgatcgcg gtgatcaact ccccggtgca
     9001 gaacttcggc agccagcgct gcttctgctc ctcgttggcc agccgcagca gatacggcgc
     9061 cacgacatcg ttgtgcagcc cgaagccgat tccgctgtag cggcccaggg tggtctcttc
     9121 ggtgatgatc gtgttgtacc ggaagtccgg atcgccgccg ccgccgtact cctccggcac
     9181 ggccatcccc agaaagccct gcttgccggc ctccagccag acgccgcggt cgacgatctt
     9241 gtcgctctcc cactgctcgt gatgcggcgc gacgtggcgc tccaagaacg cccggtacga
     9301 ctcgcggaac agctcgtgct ccggctcgaa cagcgtgcgc tcgtacttga cggcattacc
     9361 catttgtgct cctcacgtgc gcgcccccat cgcggacctc cggtatcagg aacttgccgc
     9421 ccaagatata ccaaccggat ggttggtttg ccgcgggtcg cgggcctaaa gtcggcgtcg
     9481 tggccgaaac catccgtgtc caggagcgcg gccggatcat cgagttcagc ttcgacgacc
     9541 tgatgcgcta ccacggcccg ggctcgcccg gcggcgtcgc caacgcattc aaggtgttgc
     9601 agcgcgcgtt cgcggtgttg tcgcccgacg agccaccgca ccgacgatcc gttatggtgc
     9661 gcaccgcgtt ccgtggcccg ggagcacgtg atggcgtcga ggcggtgacg cgggcggtca
     9721 ccgacggccg ctacatcgtc gacccggcgc tcacccgccc cgatctcggc cggctgcggg
     9781 aaagctttgt gttcgaagtc aggatcgccg atcgttcagt taccctgctg ctgcgcgacg
     9841 gcttcgtcac cgaggagttc atcgacctcg cgcagaaaga cgaccgcacc gacgaagatg
     9901 agt
//
LOCUS       DASWBF010000604        11641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_584284,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000604 DASWBF010000000
VERSION     DASWBF010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584284"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2484
                     /locus_tag="VF874_22450"
     CDS             <1..2484
                     /locus_tag="VF874_22450"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX9178722.1"
                     /translation="LKDEIDDDVTALRARRIVENAAQDWAAGGRDRGALLRGRQLTKL
                     SVDIGAARKVRRGRRTVHSGTDANRLSTSSRLFHRRRLATRVCLNDIGHEFLHRSIQA
                     NRARRARAIVAVVGVSALLFGSLPVAVWRQREAEMAREASVLQADATAMLAQTRPGGD
                     LEAFQKLIAANALVANADPGWLLDALISRASTSKIAEFGTTPTSVAFSADGKRMATVS
                     DRGAVQVWNAETGQPIGHPLSGGTGGVALSPDGRKVATTGANYSLLLWDADTGLPIGG
                     PLTGPTNLVTALAFSPDGQRIASADGDRTVRIWNADNGDPVGPQLTDLENVVTTLAFS
                     PVTHRLAIGDVLGNVSLLDAYSGALLSTQQPRNADKTYVSSLAFSRDGTHLVSGDGSG
                     TMVLRSGETAEPVGSPLEGHTGAVTGVAFTPDGRLFASASADQTVRVRNSATGETVGA
                     PLTGHADGVLGVEFTTNDRLVTASKDATVRFWNVDGLEPKRIGGGTGERVLLSSPDGH
                     RVVTTTSDADGFDFDWKTVRLWNADTAQVIESSLGDQPGRMGDLAFSQDGHRLATVTL
                     NPPAMRVWDTESGKPVGIQLPTDETILNWSLSPDGRRVAIDSGSAVGVWNVDNGRLLR
                     PLDRPPNVTALSLSPDGRSLATGDATGRLTLWNADTGKSMGPPVAAHVGAVSSLAFSD
                     DGTLLASGGSDGTVRLWNPQTGDPVRATLTGHKGMVSSLAFSPGRHLLASGGDDKTVR
                     LWNVDTGLPLAAPISHQEHVLDLTFSSDGRHLLSASEHGLREWPIVATSEMLCDKLTA
                     NMTHEQWRMWISPDLPYKKLCPNLPGPTS"
     gene            complement(2641..3624)
                     /locus_tag="VF874_22455"
     CDS             complement(2641..3624)
                     /locus_tag="VF874_22455"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX9178723.1"
                     /translation="MTALGDRLGRRDPPTEVPVKISKLVLSLATLLVLSAGALGAFTT
                     DSSVPSARAASVKRALFVVSNISDIPVPDGGPSGVWLSEVTEPYWVLRDAVAPQFTID
                     LASPSGGPSAIDGFSVKLNIDDWLRTDGRVTDSGDTSNERFLSDPQTRDLIQVDAVPQ
                     ADGTVQQRYSFRDTLPLSNVNVADYDVVFFVGGNAALFQFVNDQRVYGAASAAYDAGK
                     LVSAVCHGTAALLEARLPDGQYLVAGRRVTGFSNAEEDKLGQRAMAPVLLEDAFPLRG
                     ATYTAAAPPFTEHVVVDGRLITGQQPASGRAVGEAIVAYFAEPAIAAVPAA"
     gene            complement(3797..4492)
                     /locus_tag="VF874_22460"
     CDS             complement(3797..4492)
                     /locus_tag="VF874_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HEX9178724.1"
                     /translation="MKKLTAVTTVGITTVALTLALVGCGSDPKTEAQTPTSTSITTTT
                     SNAAPTTSAEAAGPNKTIQDYINENQIVETPIHRGDPGSPTINLPIPPGWSDSGPSTP
                     DWAYQAMVYDQPQVPDDPPRITAMVFKLTGNVDPAQVLEYAPGELRNLPGFEPLTNGN
                     RSTMSGFDAYSLSGNYMKDGNKRVIGRKTVVIPGQDGLYVLQMTADALADELGTVMEA
                     TTNVIDPQTTITP"
     gene            4938..5831
                     /gene="pdxS"
                     /locus_tag="VF874_22465"
     CDS             4938..5831
                     /gene="pdxS"
                     /locus_tag="VF874_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX9178725.1"
                     /translation="MDSAAQTGTARVKRGMAEMLKGGVIMDVVTPEQARIAEGAGAVA
                     VMALERVPADIRAQGGVARMSDPDLIDGIMNAVTIPVMAKARIGHFVEAQILQSMGVD
                     YIDESEVLTPADYTHHIDKWKFTVPFVCGATNLGEALRRITEGAAMIRSKGEAGTGDV
                     SNATTHMRAITGEIRRLGSLSDDELYVAAKDLQAPHELVVEVARAGKLPVTLFTAGGI
                     ATPADAAMMMQLGAEGVFVGSGIFKSDAPQHRAAAIVKATTFYDDPDVLAKVSRGLGQ
                     AMVGINVEDIAVPHRLAERGW"
     gene            5841..6686
                     /gene="tesB"
                     /locus_tag="VF874_22470"
     CDS             5841..6686
                     /gene="tesB"
                     /locus_tag="VF874_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX9178726.1"
                     /translation="MSIEEILDLEQLEVDIYRGHVFSAESNDWQRTFGGHVAGQSLVS
                     AVRTVDPRYLVHSLHGYFLRPGDAKAPTVFLVERLRDGGSFCTRRVNAIQHGQTIFSM
                     SASFQTDQSGIEHQDAMPTAPPPDNLPGLRSIKAFDEAGFRQFDEWDVCVVPTDQLER
                     TPGKASQQRVWFRHRDPLPNDPVLHICALAYMSDLTLLGSAQVHHEEERKHLQIASLD
                     HAMWFMRPFRADEWLLYDQSSPSASGGRSLTQGKIFNQYGEMVAAVMQEGLTRYKRDF
                     SPPPR"
     gene            6683..7279
                     /gene="pdxT"
                     /locus_tag="VF874_22475"
     CDS             6683..7279
                     /gene="pdxT"
                     /locus_tag="VF874_22475"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX9178727.1"
                     /translation="MNGPHVGVLALQGDTREHLAALREAGAEATTVRRRAELDAVDAL
                     VIPGGESTTMSHLLAEFELLEPLRGRLADGLPVYGSCAGMILLATDIVDAGADGRRAV
                     PLRGIDMTVRRNAFGRQVDSFEEDIPFAGLDGPVHAVFIRAPWVERVGPGVQVLASAA
                     GHPVAVRQGRMLATAFHPEITGDRRVHRLFVDILGGRA"
     gene            7332..8000
                     /locus_tag="VF874_22480"
     CDS             7332..8000
                     /locus_tag="VF874_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX9178728.1"
                     /translation="MKTPPVVSAEAWEAARQELLVKEKELTRARDALAAQRRRMPWLA
                     VEKEYVFDGPEGKATLVDLFAGRRQLIVYRAFFDPHVHGWPDHACVGCSMVADHVGNL
                     AHLNARDTTLVFASRGPQADIARLKARMGWQIPWYTITDDFDTDFGVDEWHGTNAFIR
                     DGDKVFRTYFINNRGDEVMGSTWSYLDMTALGRQEEWEDSPEGYPQSPPYEWWRWHDG
                     YGKG"
     gene            8021..8410
                     /locus_tag="VF874_22485"
     CDS             8021..8410
                     /locus_tag="VF874_22485"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9178729.1"
                     /translation="MSDTEAKEFSDRWLPAWTGNEPERLVAFYADDAFYSDPAVPGGV
                     RGRAEILAYFRRLLARYPDWVWTNTRVRPLSGGFLNHWHATIPDGERVVECDGVCTVI
                     VEDGLIRRNEVFFDRTPIMLGHDKRSG"
     gene            complement(8407..10116)
                     /locus_tag="VF874_22490"
     CDS             complement(8407..10116)
                     /locus_tag="VF874_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX9178730.1"
                     /translation="MISLNGPQTTGRDYRNLSKPQHGVRRDDNVAIPMRDGARLLADV
                     HRPDADGRYPALIAASPYPRQIQDLGAPAGLIEAGVTAFWVPRGYVHVIANVRGTGGS
                     DGTFGFFDAQERRDMHDLVEWAAAQPWCDGNVGMIGISYFAMTQLEAAVERPPHLKAI
                     FPVAVTADLYEGAIHHGLMNASFVTPFLSMIGLASERSDKLWRSAFVSVVRRILNTPR
                     LHRKFATMNGEAAVTMMRALLKLPHDPHPWDELWLDVAVKHPTRDEFWDQRDVLPMLE
                     AIDIPVYLGCDWENVPLHLPSTFAAWKALADSPNTRMGMLGQFGLTWPWESLHTEALA
                     WFDHWLKNRDTGILDGPPIRYFLPGAAEWRTADAWPPAGSVHRELALRADGTLADDEG
                     ASGRRKFMVLGAGLNRAKPSRIDPSSTLTWTSAPLDQALDMVGDIELRLVASATAIDT
                     AWIVTLLDVAPDGQSEPITAGWLRASLREVDEAASRPGAPVLPCRNAQAVPIGEDVEY
                     RIPLVPNARRFGAGHRLRLVLTSDDQDPSTPAIMNFRHASVGTSSLNTVASSSRLLLP
                     VLT"
     gene            10150..10584
                     /locus_tag="VF874_22495"
     CDS             10150..10584
                     /locus_tag="VF874_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178731.1"
                     /translation="MTMIEVEPQPRIDTLVRLPGGGEARRTEYVGTAWFDGKPMVSGL
                     MLRRSPSAPKGIRMPNLQASFFVPDSLPYSRAALGDSKLSVSVLLQGSGETGAITGAA
                     AVFTDDPSGAPTWIQVHVQGHLGWPAGVGYRIVALTPPDAVP"
     gene            10652..11407
                     /locus_tag="VF874_22500"
     CDS             10652..11407
                     /locus_tag="VF874_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9178732.1"
                     /translation="MSGHSKWATTKHKKAVVDARRGKMFARLIKNIEVAARVGGGDPA
                     GNPTLYDAIQKAKKSSVPNENIERARKRGAGEEAGGADWQNITYEGYGPNGVAVLIEC
                     LTDNRNRAAGEVRVAVTRNGGSMADPGSVSYLFSRKGVVTLDKDGLTEDDVLAAVLDA
                     GAEDVADLGDSFEVITEPTDLVAVRTALQDAGIDYESAEAGFQPSVTVPLDADGARKL
                     MKLVDALEDSDDVQDVYTNADIPDEVMAELDEE"
     gene            complement(11454..>11641)
                     /locus_tag="VF874_22505"
     CDS             complement(11454..>11641)
                     /locus_tag="VF874_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX9178733.1"
                     /translation="HGELTGIAKVEPSIRRGAVSVPHGHAEANVNLLTSKDDIDLVTG
                     MTRYSGVPVTLHPGVER"
BASE COUNT         1984 a         3776 c         3957 g         1924 t
ORIGIN      
        1 ctcaaagatg agatcgatga cgatgtaacc gctctacgag ctcggcgcat cgtggagaac
       61 gctgcgcagg attgggccgc gggcggccgc gacaggggcg cgctactacg gggccgccag
      121 ctcactaagc tgtccgtcga catcggtgcg gcccgaaagg tgcgtcgcgg ccgtcgtacc
      181 gtccacagtg gcacggacgc aaaccgattg agcacctcga gtcgattgtt tcaccgacgc
      241 cggctcgcca cgcgggtgtg cctcaacgac atcgggcatg aatttctgca ccgcagcatc
      301 caggccaatc gtgcacgtcg agcgcgagcg attgttgccg tcgtcggggt gagtgcgctt
      361 ctcttcggct ccctaccggt cgctgtctgg cggcagagag aagccgaaat ggcccgagag
      421 gccagcgttc tgcaagccga tgcgacagcg atgttggcgc agactcgtcc gggtggcgac
      481 ctcgaagcat ttcagaagct catagctgcg aacgcccttg ttgcgaacgc cgacccgggt
      541 tggctactcg acgcactgat tagccgagcg tcaacgagca agatcgccga attcggcacg
      601 acgccaacga gtgtggcatt cagcgctgac ggaaagcgca tggcaacggt cagcgaccgt
      661 ggcgccgttc aggtgtggaa tgccgagacc ggtcaaccca tcggccaccc gttgtccggc
      721 gggaccggcg gtgtggcgtt gagccccgac gggcgcaagg tcgccactac cggtgcaaac
      781 tattcgctgc tgttgtggga cgcggatacc ggactgccga tcggcggtcc gctcactggt
      841 cccaccaatc tcgtgacggc gctggcgttc agccccgacg ggcagcgcat cgcgagcgcc
      901 gacggagacc gtacggtcag gatctggaac gccgacaatg gagacccggt tggcccgcaa
      961 ttgaccgacc tcgagaacgt tgtcaccact ttggctttca gccccgtcac tcaccgtctc
     1021 gcgatcggcg acgttctcgg taacgtcagc ttgctggatg cctacagtgg cgcgctgctg
     1081 agcacgcagc aaccccggaa cgctgacaaa acctatgtct ccagcctggc gttcagccgc
     1141 gacggaaccc acctggtttc cggcgacggc agcggcacga tggtgctgcg cagcggcgag
     1201 accgcggaac cggtcgggtc cccgcttgag ggccacaccg gcgcggtgac cggtgtggcg
     1261 ttcaccccgg acggacgcct gttcgcaagt gccagcgctg atcagacggt acgggtacgg
     1321 aactccgcca ctggtgagac cgtcggcgcc ccactcaccg gacacgcgga cggtgtcctc
     1381 ggtgtcgagt tcactacgaa cgatcgcttg gtcaccgcaa gcaaggatgc aacggtgcgg
     1441 ttctggaatg tcgatgggtt ggaacctaaa cggatcggcg gtggaactgg tgaaagggtc
     1501 ttgctctcaa gtccggacgg gcaccgcgtt gtaaccacga cgagcgacgc cgacggcttc
     1561 gattttgatt ggaagaccgt gcggctgtgg aacgccgata cggctcaagt catcgaatcg
     1621 tcgctcggcg atcaaccggg ccgcatgggc gacctggcat tcagccaaga cggccatcgc
     1681 ctggccactg tgactttgaa tccgcccgcc atgcgcgtct gggacaccga gtcgggcaag
     1741 ccggtcggca tccagttgcc gacggacgag accatcctga actggtcatt gagccccgac
     1801 ggccgtcgcg tcgccatcga ttccggcagc gcggtggggg tgtggaatgt ggacaatggc
     1861 cgcttgctcc gacccttaga tcgcccgccg aacgtcacag cactcagtct cagccccgat
     1921 gggcgttcgc tggccaccgg cgatgccacc ggacgcctca cgctgtggaa cgcggatacc
     1981 gggaaatcga tggggccgcc cgtggcggct cacgtaggcg cggtcagcag tctggctttc
     2041 agcgatgacg gaaccttatt ggccagcggc ggctccgatg gcaccgtgcg actctggaat
     2101 ccccaaaccg gcgatcccgt ccgcgccacg cttaccggtc acaaagggat ggtctccagt
     2161 ctcgcattca gtcccggccg gcacctcctc gccagcggcg gcgacgacaa gaccgtgcgg
     2221 ctgtggaacg tcgacaccgg tttgccgctc gcggctccca ttagccacca agagcatgtg
     2281 ctcgacctca cgttcagtag cgatggccgg cacctgctgt ctgccagcga gcatggcttg
     2341 cgcgaatggc ccatcgtagc cacatcggag atgctgtgcg acaaactgac tgccaatatg
     2401 acccatgagc agtggcggat gtggatttcc ccggacctgc cttacaagaa gttgtgcccc
     2461 aatttgcccg ggccaacctc gtaaaggaaa tatccgattt acagcccggc ggtggccgta
     2521 ccctcccgga agatagacat caacatcgct atgaccggtg tgtgcctgcg cgatgcgctc
     2581 gatctcgatg acggcatcgc cgtctgtgcc aactgccaat ggctgaacac caagcgcgaa
     2641 tcacgccgcg ggcacggcgg caatcgccgg ctcggcgaag taggcgacga tcgcctcgcc
     2701 gacggcgcgc cccgatgccg gctgctggcc cgtgatcagg cggccgtcca ccacgacgtg
     2761 ctccgtgaag ggtggtgcgg cggcagtgta tgtggcgccg cgcaggggga atgcgtcctc
     2821 gaggaggacc ggcgccatcg cgcgctggcc tagcttgtcc tcctcggcgt ttgagaaccc
     2881 ggtgacgcgc cggcccgcga ccaggtactg cccatcggga agcctcgcct cgaggagcgc
     2941 cgccgtgccg tggcacaccg cgctgaccag cttgccggcg tcgtacgcgg cgctcgctgc
     3001 gccgtagacg cgctggtcgt tgacgaactg gaacagcgcg gcgttcccgc cgacgaagaa
     3061 caccacgtcg tagtcggcca cgttcacgtt cgaaagcggc agcgtgtcgc ggaacgagta
     3121 gcgctgctgc acggtgccgt cggcctgcgg caccgcgtcg acctggatga ggtcgcgcgt
     3181 ctggggatcg gagaggaagc gctcgttgct cgtatccccg gagtccgtaa cgcggccgtc
     3241 ggtgcggagc cagtcatcta tgttcagctt gacgctgaag ccgtcgatcg ccgacggccc
     3301 gccgctgggg ctcgccagat cgatagtgaa ctgcggcgcg acagcgtcac gcagcaccca
     3361 gtacggctcc gtgacctcgc tgagccagac acccgagggg ccgccgtccg gcacggggat
     3421 gtcgctgatg ttcgagacca cgaacagcgc gcgcttgacg gacgcggctc gcgccgatgg
     3481 cacgctggaa tcggtggtga acgccccgag ggcgccggcg gacagcacga gcaaggtcgc
     3541 gagcgacaag accaacttcg atatcttcac gggaacctcc gtcggcgggt cgcgccggcc
     3601 caggcgatca ccgagcgccg tcatgaacaa cgagactagc ggcccaaggc gtggtcagaa
     3661 gccattctca gcggtattgc agcttttcca cgggccctcg atcgccgcgg aaaggccccg
     3721 cgagggggtc agctcgtgct caagtggatc gcaccggcgt gtgcggtctg gttaccactc
     3781 ggggcggcgg ccgtcgtcag ggagtgatgg tggtctgcgg gtcgatgacg ttggtggtgg
     3841 cctccatgac agtacccagt tcgtccgcga gagcgtcggc agtcatctgg agtacgtaca
     3901 ggccgtcctg accggggatc accaccgtct tccgcccgat cacgcgcttg ttgccgtctt
     3961 tcatgtagtt gccggacagc gagtaggcgt cgaagccact catggtgctc ctgttcccgt
     4021 tggtcaacgg ctcgaagccg ggcagattcc tcaactcgcc cggcgcatat tcgaggacct
     4081 gcgcagggtc aacgttgccg gtgagcttga agaccatcgc ggtgatgcgc ggggggtcgt
     4141 ccggcacctg tggctggtcg taaaccatcg cctggtaggc ccagtctggt gtgctggggc
     4201 cggagtctga ccaccccggc gggatcggca agttgatggt gggagagccg ggatcgccgc
     4261 ggtggatcgg cgtctcgacg atctggttct cgttgatgta gtcctggatc gtcttgttgg
     4321 ggccggcagc ctcggctgat gtggtcggcg cggcattgga tgtcgtcgtg gtgatcgacg
     4381 tcgacgtggg cgtctgcgcc tcggtcttgg ggtcggagcc gcagccaacg agcgcgaggg
     4441 tcagagcgac agtggtgatg cccacagtgg tgactgccgt cagtttcttc atggggtttg
     4501 gtgcctcctg tcgtccgggg tctatggcgc agcctacggc ggcagactgg tgacggaagc
     4561 caaatccccg cggagcgtag cagtgtccga tttgaagctg atcggaattt ccggcacttc
     4621 tcgcggacgc gcgggggcca gcgggctagt tgggatggca gaggtaggag cctgccaccg
     4681 cggccgtacc gcaccagcgc ggcgcactga cgcgtttgcc aacgcggcca tgcccatctg
     4741 ccggtgcatg cgtcattgct cgaccaggtc gagatctact tctcggtgat acagacaaca
     4801 aagcgccgac agtgcatcca gctacgcgac acgccgtggc ggcgtcgcca gattcacact
     4861 cgacgctgtg cccgaccaga aaaccgctgg tggccgacca ctagactggg gcaagaacca
     4921 gtggaggaga ggccgtagtg gacagcgctg ctcagaccgg aaccgcgcgg gtcaagcgcg
     4981 gcatggccga gatgctcaag ggcggcgtga tcatggacgt cgtcaccccc gagcaggccc
     5041 gcatcgccga aggcgccggc gcagtcgccg tgatggcgct ggaacgcgtg cccgccgaca
     5101 tccgtgcgca gggcggcgtg gcacggatga gtgaccccga cctcatcgac ggcatcatga
     5161 acgccgtcac gatcccggtg atggccaagg cccgcatcgg ccacttcgtc gaggcccaga
     5221 tcctgcagag catgggcgtg gactacatcg acgagtccga ggtgctgacc cccgccgact
     5281 acacccatca catcgacaag tggaagttca ccgtgccgtt cgtgtgcggg gccaccaacc
     5341 tcggcgaggc gctgcggcgg atcaccgaag gggccgcgat gatccgctcc aagggtgagg
     5401 cgggcaccgg tgacgtgtcc aacgcgacca cgcacatgcg ggcgatcacc ggcgagatcc
     5461 gcaggctggg ctcgctgtcc gacgacgaac tctacgtcgc ggccaaggac ctgcaggcgc
     5521 cccacgaact ggtcgtcgag gtggcccggg caggcaagct gccggtgacg ctgttcaccg
     5581 ccggcggcat cgcgaccccg gccgacgcgg cgatgatgat gcaactcgga gccgagggcg
     5641 tattcgtcgg ctccgggatc ttcaagtccg acgcgccgca gcatcgcgcc gccgcgatcg
     5701 tcaaggcgac aacgttctac gacgatcccg atgtgctggc caaggtgtcg cgcggcctgg
     5761 ggcaggcgat ggtcgggatc aacgtggagg acatcgcggt cccgcatcgg ctcgccgagc
     5821 gcggctggta atccttacgc atgtcgatcg aagagatcct tgatctcgag caactcgagg
     5881 tcgacatcta ccgcggccac gtgttcagtg ccgaatcgaa cgactggcag cgcacgttcg
     5941 gcggtcatgt cgcggggcag tcgttggtct ccgccgtgcg caccgtcgac ccgcgctatc
     6001 tcgtgcactc tctgcatggc tacttcctgc ggcccggaga tgccaaggcg cccaccgtgt
     6061 ttctcgtcga acgcctccgc gacggcggct cattctgcac ccggcgggtg aacgccattc
     6121 agcacggcca gacgatcttt tccatgtcgg cgtcctttca gaccgaccag agcggtatcg
     6181 aacaccagga cgcgatgccc acggcgccgc cacccgacaa cctgccgggg ctgagatcga
     6241 tcaaggcgtt cgacgaagcc ggcttccggc agttcgacga atgggacgtc tgcgtcgtcc
     6301 cgacggatca gctggaacgc accccgggca aggcctccca acagcgggtg tggttccggc
     6361 accgcgaccc gctgccgaac gacccggtgc tgcacatctg cgcgctggcc tacatgagcg
     6421 atctgacgct gctgggctcg gcgcaggtgc atcacgagga ggagcgcaag cacctgcaga
     6481 tcgcgtcgct cgatcacgcg atgtggttca tgcgcccgtt ccgggccgac gagtggctgc
     6541 tgtatgacca gtcctcgccg tctgcatcgg gcggtcgctc gttgacgcag ggcaagatct
     6601 tcaaccagta cggcgagatg gtggcggcgg tgatgcagga ggggctgacc cgctacaagc
     6661 gcgacttttc gccgccaccc aggtgaacgg gccgcacgtg ggcgtccttg ccctgcaagg
     6721 ggatacccgc gaacatctgg cggcgttgcg tgaggcgggg gcggaggcga cgacggtgcg
     6781 ccgccgagcc gagctcgacg ccgtcgacgc gctggtgatt cccggcggcg aatccaccac
     6841 gatgagccac ctgctggccg agttcgagtt gctcgagccg ctgcgcgggc ggctggccga
     6901 cggcctgccg gtgtacgggt cctgcgcggg gatgatcctg ctggccaccg acatcgtcga
     6961 tgccggcgcg gacggccgcc gcgccgtgcc gttgcgtggc atcgacatga cggtgcgccg
     7021 caacgctttt gggcgccagg tcgactcgtt cgaggaggac atcccgttcg cgggcctcga
     7081 cgggccggtg cacgcggtgt tcatccgcgc gccgtgggtt gagcgcgtgg ggcccggtgt
     7141 gcaggtgctg gcttctgcag cgggtcaccc ggtggcggtt cggcagggcc gcatgctggc
     7201 cactgcgttt catccggaga tcaccggcga ccggcgcgtg caccggctgt tcgtcgacat
     7261 actcgggggc agggcctgaa gccgcgcagg gacgggctga attccctatc aaccagaagg
     7321 agacaccgcg aatgaagaca cccccggttg tatcggcgga agcgtgggag gctgcccgcc
     7381 aggaactact ggtcaaggaa aaggaattga cccgtgcgcg tgacgcgctt gccgcccagc
     7441 gtcgacggat gccatggctg gccgtggaga aggagtacgt gttcgacggg ccggagggaa
     7501 aggcgactct ggtggacctc ttcgcgggcc ggcgtcagct gatcgtctat cgcgcgttct
     7561 ttgatcccca cgtgcacggc tggccggacc acgcttgcgt cggctgctcg atggtggccg
     7621 accatgtcgg taacctcgcc cacctgaacg cccgcgacac cactctggtg ttcgcgtcgc
     7681 gcgggccgca agctgacatc gcgcgcctga aggcccgcat gggctggcag ataccgtggt
     7741 acaccatcac agacgacttc gacacggact tcggagtgga cgaatggcac ggcacaaacg
     7801 cattcattcg tgacggcgac aaggtgtttc gcacctactt cattaacaac cgtggagatg
     7861 aggtgatggg tagcacctgg agctacctcg acatgacggc gcttgggcgt caggaagagt
     7921 gggaggattc accggagggc tatccgcaaa gtccgcccta cgaatggtgg cgctggcacg
     7981 acggatacgg caagggctag ccatgcaagc gttttccgga atgtccgaca cggaggccaa
     8041 agagttcagc gatcgatggc tgcctgcctg gactgggaac gaacccgagc gcctcgtcgc
     8101 cttctatgcc gacgatgcct tctacagtga tccggcagta ccgggtggcg tgcggggccg
     8161 agccgagatc ctcgcgtact ttcggcgact cctggcccgc tacccggact gggtttggac
     8221 gaacacccgg gtgcgaccgc tcagcggtgg gttcttgaac cactggcatg caacgattcc
     8281 ggacggtgag cgggtggtcg agtgcgacgg cgtatgcacg gtgatcgttg aggacgggct
     8341 gatccgccgt aacgaggtct tcttcgaccg gacgccgatc atgttgggac acgacaagag
     8401 atcaggctaa gtgagcaccg gcagcaacag ccgtgacgac gacgccacgg tgttcaggct
     8461 gctggtgccg acgctggcgt gccggaagtt catgatcgcc ggcgtcgacg ggtcctggtc
     8521 gtcgctggtc agcaccagcc gcaggcggtg gccggcccca aaccgacggg cgttgggcac
     8581 caacgggatt cggtactcga cgtcctcacc gatcggtacc gcctgcgcgt tgcggcacgg
     8641 cagcacgggc gcgcccggcc gactggcggc ctcgtcgacc tcacgcaggc tggcgcggag
     8701 ccagcccgcg gtgatgggct cggattgccc gtcgggtgcc acgtccagca gtgtgacgat
     8761 ccacgccgtg tcgatcgccg tcgccgaggc aaccagccgc agttcgatgt cgccgaccat
     8821 gtcgagcgcc tggtccagcg gcgcgctggt ccacgtcagc gtcgatgaag ggtcgatccg
     8881 gctgggcttg gcccggttca ggcccgcgcc cagcaccatg aacttccggc gaccggatgc
     8941 accctcgtcg tcggccagtg tgccgtcggc gcgcagcgcc aactcgcgat gtaccgatcc
     9001 ggccggtggc cacgcgtcgg cggtgcgcca ctcggccgcg cccggcaaga agtagcggat
     9061 cggcggcccg tcgagaatcc cggtgtccct gttcttcagc cagtggtcga accaggccag
     9121 ggcctcggtg tgcaggctct cccacggcca ggtcaggccg aactgtccga gcatccccat
     9181 gcgcgtattc gggctgtccg ccaaggcttt ccacgccgcg aacgtcgacg gcagatgcag
     9241 cggcacgttc tcccagtcac accccaggta caccgggatg tcgatcgcct cgagcatcgg
     9301 caacacatcg cgctgatccc agaactcgtc ccgcgtcggg tgtttgaccg cgacgtccag
     9361 ccacaactcg tcccagggat gcgggtcgtg cggcagcttg agcagcgcgc gcatcatcgt
     9421 gaccgcggcc tcgccgttca tcgtggcgaa cttgcggtgc aggcgcggcg tgttcaggat
     9481 tcggcggacc actgagacga acgcgctgcg ccacagcttg tcgctgcgct cggacgccag
     9541 tccgatcatc gacaagaacg gcgtgacgaa cgacgcgttc atcagcccgt ggtgtatcgc
     9601 cccctcgtac aggtccgcag tgacggccac cgggaagatc gccttcaggt gcggcggccg
     9661 ctcgactgcc gcttcgagct gcgtcatcgc gaagtagctg atcccgatca tcccgacgtt
     9721 gccgtcacac cacggctgcg cggccgccca ctcgacgagg tcgtgcatgt cgcggcgctc
     9781 ctgggcgtcg aagaagccga aggtgccatc cgagccgccg gtgccgcgca cgttggcgat
     9841 gacgtggacg tagccgcgcg gcacccagaa cgcggtgacg ccggcttcga tcaaaccggc
     9901 cggcgcgccg aggtcctgaa tctggcgggg atacggcgag gccgcgatca gcgcggggta
     9961 acgtccgtcg gcgtcggggc ggtgcacgtc ggccaacagc ctggcgccgt cgcgcatcgg
    10021 gatcgccacg ttgtcgtcgc gccgcacccc gtgctgcggc ttgctgagat tgcggtagtc
    10081 gcgtccggtg gtctgcgggc cgttcagact gatgatctgc accctttcgt cctacacgtg
    10141 aggtaatcga tgacgatgat cgaggtggag ccgcagccgc gcatcgacac actggtgcgc
    10201 cttcccggcg gcggcgaggc ccgccgcacc gaatacgtcg gcacggcgtg gttcgacggt
    10261 aagcccatgg tgtcagggct tatgcttcgc cgctccccga gcgcgccgaa gggaatcagg
    10321 atgccgaacc tccaggcgtc gttcttcgtc cccgacagcc tgccgtactc gcgggccgcg
    10381 ctcggcgact cgaagctcag cgtctcggtg cttctgcagg gttccggcga gacgggtgcg
    10441 atcaccggag ccgccgcagt gttcaccgac gacccgtcgg gcgcgccgac gtggatccag
    10501 gtgcacgtgc agggccacct gggctggccc gctggcgtcg ggtaccggat cgtcgcgctg
    10561 acgccgccgg atgcggtgcc gtagcccggc ataaccgtcc acgtagactc gttgaccgga
    10621 ctttgcagtt gagaaacgag gtagtacctg catgagcggc cactccaaat gggccacgac
    10681 gaagcacaag aaggctgtcg tcgacgcgcg ccgcggcaag atgttcgccc ggctgatcaa
    10741 gaacatcgag gtggccgcga gggtcggcgg aggcgacccg gcgggcaacc cgacgctgta
    10801 tgacgcgatc cagaaggcca aaaagagctc ggtgcccaac gagaacatcg agcgggcccg
    10861 caagcgcggg gccggcgagg aggccggcgg tgcggactgg cagaacatca cctacgaggg
    10921 ctacgggccc aacggcgtcg cggtgctgat cgagtgcctg accgacaacc gcaaccgcgc
    10981 ggccggcgag gtccgcgtgg cggtgacccg caacggcggc agcatggccg accctggatc
    11041 ggtgtcgtac ctcttctccc gcaagggcgt ggtgacgctg gacaaggacg ggctgaccga
    11101 ggatgacgtg ctggccgcgg tgctcgatgc gggcgccgaa gacgtcgccg acctcggcga
    11161 cagcttcgag gtgatcaccg agccgaccga cctcgtcgcg gtgcgcaccg cgctgcagga
    11221 cgccggcatc gactacgagt ccgccgaggc ggggttccag ccgtcggtca ctgtgccgct
    11281 ggacgccgac ggcgcgcgca agctgatgaa gctcgtcgac gcgctcgagg acagcgacga
    11341 cgtgcaggac gtctacacca acgccgacat tcccgacgag gtgatggccg agctcgacga
    11401 ggagtaggtc gccgttttcg gcgaaaccgg tgtttcgcac gcctgcactc gagtcatcgt
    11461 tctactcctg gatgcaacgt cactggaact cctgagtagc gcgtcatgcc ggtcaccagg
    11521 tcgatgtcgt ccttcgacgt gagcaggttg acgttggcct cagcgtggcc gtgcggcacc
    11581 gacaccgcac cccgccttat cgacggctcc accttcgcga tcccggtcag ctcgccgtgc
    11641 t
//
LOCUS       DASWBF010000605         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_585733,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000605 DASWBF010000000
VERSION     DASWBF010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585733"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1716)
                     /locus_tag="VF874_22510"
                     /pseudo
     CDS             complement(260..1716)
                     /locus_tag="VF874_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            1774..>2879
                     /locus_tag="VF874_22515"
     CDS             1774..>2879
                     /locus_tag="VF874_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX9178734.1"
                     /translation="MGDDQDHWRRYPFELVPGDPQFRFPAAEAEHADYESDTWFLAGE
                     LTGDSGRKFAFLTIFNKNRPGHTVVADFYTFALFDLDNGTYGTYTDYDMPPANLGAAP
                     KLSTAQGHLDMTYESSAGAAVWRTCRDEQDRLLPYTYDVALVGADAAGQPMTLRLHVT
                     PTRAPVPLGASTYNGKIACFGQDDTYSYFQTGMTMTGTLQWGSVEEAVSGSAGHVDRQ
                     WFPRYAGGGGTGGDPRARAHEWRTINLDNGLDLSIWRQFDRTNHNALQPFSGATTSSD
                     NAEPQCAEDIEVDVTSYVRWPESVRTLVPPLAAVRYMPDRHRITSQQLDFDLTGEPLV
                     AAPAHALPIEYMEGPYWYHGTLGGKPVSGFAFYER"
BASE COUNT          491 a          934 c          951 g          503 t
ORIGIN      
        1 cgcgcccggg tgaccgacaa gcggtccgag aacctcaacg gcgccacgcg ataccttgtg
       61 gacttggatg tcgacgtgtg caaccaacgg ggcgaactgt gctgccccgc gaaagtgacg
      121 ttggaaatga tcagctgggt gccacacacg ccccatcagc accttgggca ctagctgcgg
      181 gtaggtcgat gccgggcttc cgtcatcaat tgatgacgcg actcagacat gaccatgact
      241 cgaagagagc caaggccggt catgctaagg gcggaagctc gccggcgctg accgtcgact
      301 gcaactcgac gtattccgac aacccttccg cgccgaactc gcgtccgatg ccggactgct
      361 tgaatccgcc gaacggacaa ctggtgtcga gcgtggacat gttgatgccg taggtgccgg
      421 tgcggatctg ctcggcgatc tcaaggccgt gcgcggtgtc ggcggtccac acggaccccg
      481 cgagcccgta gtcgctgtcg ttggcgatct gcaccgcgtc acgctcgtcg gagtacgtca
      541 gcacggtcac cacgggaccg aagatctctt cgcgggcaat gcgcatctcg ttggtcgcgc
      601 tggtgaacaa cgtgggacgg acgtaccagc cgcggtcgta cggtgtgtcg gtgccgccga
      661 cgacgatgcg cgcgccttcg tcgagcccgg cctggatgta gccctgcacc cggtgctgct
      721 ggcgttgcgc gacaaggggt ccgatgtcgg tggcgtcgtc ggcggggtcg ccgacctgaa
      781 ggctcgacat catctcggcc agagcgtcga cgacctcgtc atggcgacgc tcgctgacca
      841 gtacccgggt ctgggcgacg cacgcctgtc cgttgttcat caggctggcg gtcttgaggc
      901 ccttgacggt gtggtcgagg tcggcgtcgt cgagaatgat cgccgccgac ttgccgccga
      961 gttcgaggct gacccgcttg agctgttctc cgcacagcgc ggcgatcctg cggccggtgg
     1021 ccgaggaacc ggtgaacgag atcttgtcca cgccggggtg tcggaccagg ttctcaccag
     1081 tttctcggcc gccggggatg atcgacacca cgccttcggg taggtcgatc tgttcgagca
     1141 tctcggcgag ccacagcgca tcgagcggtg tttcgggtgc cggcttgacg accaccgtgc
     1201 aaccggcgat cagcgccggg atcagcttgg gcatgatcag gaattgcggc acgttccacg
     1261 gcacgatcgc gccgacgacg ccgacggggg cgcggcgcac atggacctcg ccgaacaagc
     1321 cctggcgccg ctccgcccac cgcaactcgc gcgcgctcgc catggcaagg tgcatctgcg
     1381 ccagcgcccc ggtcgcctgg ccgagccgac tgaagctgcg ggtagtgccc atctccgcgg
     1441 tgatgaggtc ggccatctcg tcggtgtgag tcgagtagac cgtggtgagc ttgtcgatct
     1501 tgtccatccg ctcgccgtga gatacgcggg gccacgggcc gttgtcgaag gcatcccggg
     1561 cggcggtgac ggccttgtcg acgtcggtgg cggtcgcctc cggagcctcg ccgatcggct
     1621 gttcggtgtg ggggaaatca ccgtgatctt ctgaccgctg gacggtggct gccagcggct
     1681 gccgatgaaa agctgttcgt actcaaggga tttcatgcca gtcaccgccc tgtcaccccg
     1741 gtttgaggtc gctattgctg ctaacatagc aaaatgggcg atgaccagga tcactggcgt
     1801 cgttatccgt tcgagctggt gccgggcgac ccgcagttcc gcttcccggc cgccgaggcg
     1861 gagcacgccg actacgagtc ggacacttgg tttctggccg gagagctgac gggggacagt
     1921 ggccggaagt tcgcgttcct gacgatcttc aacaagaacc gtcccggcca caccgtggtc
     1981 gccgacttct acacgttcgc gctgttcgac ctcgacaacg gcacctacgg cacgtacacc
     2041 gactacgaca tgccgcccgc caatctcggc gctgcaccca aactgtccac cgcgcagggc
     2101 catctcgaca tgacctacga aagcagcgcc ggcgcagcgg tgtggcgaac gtgccgcgac
     2161 gagcaagacc gactgctgcc ctacacctac gacgtcgcac tcgtcggcgc cgatgcggcc
     2221 ggccaaccga tgacgttgcg tctgcatgtc acacccacgc gtgccccggt gccactcggc
     2281 gcgtcgacct acaacggaaa gatcgcctgc ttcgggcagg acgacacgta ctcctacttc
     2341 cagaccggca tgacgatgac cggaaccctg cagtggggtt ccgtggagga agctgtctcg
     2401 ggcagcgctg gtcacgtcga ccgccagtgg tttccgcggt atgccggcgg tgggggcacc
     2461 ggcggagatc cgcgcgcacg cgcacacgag tggcgcacga tcaacctcga caacggtctg
     2521 gacctgagca tctggcggca gttcgaccgg actaaccaca atgcgctgca accgttttcc
     2581 ggtgcgacga cgagttcgga caatgccgaa ccacagtgcg ccgaggacat cgaggtcgat
     2641 gtcaccagct acgtccgctg gccggaatcg gttcgcaccc tggtgccgcc gttggccgcg
     2701 gtgcggtaca tgccggaccg gcatcggatc acgtcacaac agctcgactt cgacctgacc
     2761 ggagagccgt tggtggccgc cccggcacac gcgctgccga tcgaatacat ggaaggtccg
     2821 tactggtatc acgggacgct gggcggaaag ccggtgagtg ggtttgcgtt ctacgagcg
//
LOCUS       DASWBF010000606         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_585870,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000606 DASWBF010000000
VERSION     DASWBF010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585870"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2910)
                     /locus_tag="VF874_22520"
     CDS             complement(<1..>2910)
                     /locus_tag="VF874_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX9178735.1"
                     /translation="PSARMPKLTRRSRILIAVALVVVALLLVGPRFIDTYVDWLWFGE
                     LGYRSVFTTVLWTRLVVFFVAGLLVGAIVYAGLALAYRTRPVFVPANGPNDPVARYRT
                     AVMTRLRLFGIGIPAVIGLFAGFIAQSYWARVQLFLHGGDFGVADPQFGRDLGFYAFD
                     LPFYRLVLSYLFVAVFLALLANLVSHYIFGGIRLSGRTGAVSRSARIQLVSLVGIFVL
                     LKAVAYWLDRFELLSHTRGGKPFTGAGYTDINAVLPAKMILLAIAIICAVAVFSAIVL
                     RDLRIPAIGLVLLLLSSLIIGAGWPLIVEQFSVKPNAAQKEKDYIERSIRATRQAYGL
                     TDDVVTYRDYSGNTAATAQQVAADQATTSNIRVLDPTIISPAFTQFQQGKNFYTFPDQ
                     LSIDRYVGPDGNLRDYVVAARELNPDRLIDNQRDWINRHTVYTHGNGFIASPANTVRG
                     IANDPNQNGGYPEFLVNVVGANGAVQSQGPAPLDQPRIYFGPVIAGATADYAIVGKGG
                     VDREYDYENNTETKNYTYSGTGGVPVGDWLSRTVFAAKFAERNFLFSNVIGDNSRILF
                     NRDPAQRVQAVAPWLTTDSSMYPAIVNKRMVWIVDGYTTLDNYPYSQLTSLSSATADS
                     TEVAVNRLAPDKQVSYIRNSVKATVDAYDGTVTLYAQDENDPVLKAWMAVFPGTVKPK
                     SEISADLQAHLRYPEDLFKVQRMLLGKYHVNEPVPFFNASDFWDVPLDPNPTASSYQP
                     PYYIVAKDLARNDSSSSFQLTSAMNRFKRDFLAAYISASSDPDTYGKLTVLTIPGQVN
                     GPKLANNAITTDTAVSQYLGVIGRDNQNRIRWGNLLTLPIGQAGLLYVEPVYASPGTS
                     DAASSYPRLIRVAMMYNDRVSFGPTVRDALEQLFGPGAGATATGPAPAPEGTPSAAPP
                     STPVPAVVPPDGNPTAQPPAATPVPAAVPPGVPLQLAAQKAAALQEVQNALGALQ"
BASE COUNT          525 a          927 c          955 g          503 t
ORIGIN      
        1 gttgcagcgc gccgagcgcg ttttgaacct cctgcaacgc cgcagccttc tgcgccgcca
       61 actgcagcgg caccccgggt ggcaccgcgg ccgggaccgg tgtggccgcc ggcggctgcg
      121 cggtgggatt gccgtcgggc ggaacgactg cggggaccgg cgtggacggc ggcgccgcgg
      181 acggcgtgcc ttccggcgcg ggcgccgggc ccgtcgcggt cgcgcccgcg cccgggccga
      241 acagctgctc cagagcgtct cgcaccgtcg gcccgaagct gacccggtcg ttgtacatca
      301 tcgcgacgcg aatgagcctc gggtacgacg acgccgcgtc gctggtgccc ggtgaggcgt
      361 acacaggttc gacgtagagc aagccggcct gcccgatcgg cagcgtcagc agattgcccc
      421 accggatccg gttctggttg tcccgaccga tcacacccag gtattgcgac accgcggtgt
      481 cggtggtgat cgcgttgttg gccagcttgg gcccattgac ctgacctgga atcgtcagca
      541 ccgtgagctt gccgtacgta tcaggatccg aactggcgct gatgtaggca gccaagaagt
      601 cgcgcttgaa ccggttcatc gcactcgtca actgaaacga tgacgaactg tcgttgcgcg
      661 caaggtcttt ggcgacgatg tagtacggcg gttgatagct gcttgccgtc gggttcgggt
      721 cgagcgggac atcccagaaa tctgacgcgt tgaagaaggg caccggctcg ttcacgtggt
      781 acttgcccag cagcatgcgc tgcaccttga acaagtcctc cggataacgc agatgcgcct
      841 gcaagtcggc cgaaatctcg cttttcggct tcacggttcc ggggaacacc gccatccacg
      901 ccttcagcac cgggtcgttc tcgtcctgcg cgtacagcgt cacggtgccg tcgtaggcgt
      961 ccacggtcgc cttcacggag ttgcggatat acgacacctg cttgtctggc gccaagcggt
     1021 tcaccgccac ttccgtggaa tcggcggtcg ccgacgacag cgatgtcaac tgggaatagg
     1081 gatagttgtc caaggtcgtg tatccgtcga cgatccatac catccgcttg ttcacgatcg
     1141 ccgggtacat cgagctgtcg gtggtcagcc acggagcgac ggcctgcaca cgctgggctg
     1201 ggtcacggtt gaacagaatc ctgctgttgt cgccaatcac attggagaac aggaagtttc
     1261 gttcggcgaa cttcgccgcg aacaccgtgc gggagagcca gtcgccgacc ggcacgccgc
     1321 cggttccgct gtaggtgtag ttcttggtct cggtgttgtt ctcgtagtcg tactcgcggt
     1381 caacgccacc cttgccgacg atcgcgtaat cagctgtggc gccggcgatc accggcccga
     1441 aatagatgcg cggctggtcc agcggggcgg gaccttgaga ctgcaccgcg ccgttcgcgc
     1501 cgacgacgtt gactaggaac tcggggtatc cgccgttctg gttgggatcg ttcgcgattc
     1561 cacgcaccgt gttcgccggc gacgcgataa acccgttgcc gtgggtatag acggtgtgcc
     1621 ggttgatcca gtcgcgctgg ttgtcgatca gccggtcggg gttgagttca cgcgcggcga
     1681 cgacatagtc acgaagattg ccgtccgggc ctacataacg gtcgatcgac agctggtcag
     1741 ggaaggtgta gaagttcttg ccctgctgga actgcgtgaa cgccgggctg atgatcgtcg
     1801 ggtccagcac cctgatattg gaggtcgtag cctggtcggc ggccacctgc tgcgcggttg
     1861 ccgcggtgtt tccgctgtag tcgcggtacg tcaccacgtc gtcggtcagg ccgtaggcct
     1921 gcctcgtcgc acggatactg cgctcgatgt aatccttttc cttctgcgcc gcattgggtt
     1981 tgacgctgaa ctgctcgacg atcaatggcc agcccgcgcc gatgatcaac gatgacagaa
     2041 ggagcaacac cagccctatc gcgggaatcc gcaagtcacg caacacgatc gccgagaaca
     2101 cggcgaccgc gcagatgatc gcgatcgcga gcaggatcat cttggcgggc agcaccgcgt
     2161 tgatgtcggt gtagccggcc ccggtgaacg gcttgccgcc gcgggtgtgc gacagcagct
     2221 cgaagcggtc cagccagtac gcgacggcct tgagcagcac gaaaattccc acaagcgaca
     2281 ccagctggat tcgggcggag cggctcactg cgccggtgcg gcccgacagc cggatgccgc
     2341 cgaagatgta gtggctgacg aggtttgcca acagcgcgag gaacaccgcg acgaacaggt
     2401 agctcagcac cagccgatag aacggcaggt cgaacgcgta gaacccgagg tccctgccga
     2461 attgcgggtc ggcgacgccg aagtcaccgc cgtgcaggaa caactgcacg cgggcccagt
     2521 agctctgcgc gatgaagccg gcgaacaggc cgatgactgc cgggatgccg atgccgaaca
     2581 gccgcaaccg cgtcatcacg gcggtgcgat agcgtgccac cgggtcgttg gggccgttcg
     2641 cgggcacgaa caccggccgc gtgcgatacg ccagcgccag cccggcatac acgatggccc
     2701 cgaccagcag ccccgcgacg aagaacacca cgagcctggt ccacagcacc gtcgtgaaga
     2761 ccgaccgata gcccagctcc ccgaaccaca gccagtcgac gtacgtgtcg atgaaccgag
     2821 gccccaccag cagcaacgcg acgacaacga gcgcgacggc gatcagaatt cggctgcgcc
     2881 tggtcagctt gggcatcctg gcggacggcc
//
LOCUS       DASWBF010000607         8301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_586096,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000607 DASWBF010000000
VERSION     DASWBF010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8301
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586096"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..408
                     /locus_tag="VF874_22525"
                     /pseudo
     CDS             127..408
                     /locus_tag="VF874_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878889.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodehydratase"
     gene            418..1782
                     /locus_tag="VF874_22530"
     CDS             418..1782
                     /locus_tag="VF874_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX9178736.1"
                     /translation="MPLRCGGVCAVGDDGEVADIKRRGAARNAKLAGLGAGMAGRAAL
                     GFGKRLTGKSKDEVNAELMDKAAQQLFTVLGELKGGAMKVGQALSVMEAAIPERYGRP
                     YREALTKLQKDAPPLPAAKVHRVLDQQLGTKWRERFLSFDDTPAASASIGQVHKAVFH
                     DGREVAVKIQYPGADEALRADLKTMQRMVGVLKQLSPGADVDGVVDELIERAEMELDY
                     RLEAENQRAFAKAYEGDPKFLIPHIVASAPKVLVAEWIEGIPMSQIIRSGTVEQRDLM
                     GTLLAELTYSAPKRLEMMHGDAHPGNFMLMADGKMGVIDFGAVAPLPGGLPIELGHML
                     RYALDKDYDNLAATMEKIGFIQPGEKVPVREIDEMLCQYVEPVQAEVFHFTRKWLQRM
                     TAVQMDKSVAQIKTARQLDVPPKLAIPMRVIASNVAIACQLDARVPIKSIALQYIPGF
                     VDAA"
     gene            complement(1854..2123)
                     /locus_tag="VF874_22535"
     CDS             complement(1854..2123)
                     /locus_tag="VF874_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9178737.1"
                     /translation="MSLSTCRARVWTELPCHVGDPDLWFAENPDDLERAKQLCADCPI
                     RRQCLQAALEREEPWGVWGGQIFDRGVVVARKRPRGRPRKQAAAA"
     gene            complement(2254..2421)
                     /locus_tag="VF874_22540"
     CDS             complement(2254..2421)
                     /locus_tag="VF874_22540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178738.1"
                     /translation="MNIFAVGMRVAGAARALPAATWTAHAAASAHATAPAAPAQRKRR
                     TATGFVDGGID"
     gene            complement(2611..4710)
                     /locus_tag="VF874_22545"
     CDS             complement(2611..4710)
                     /locus_tag="VF874_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEX9178739.1"
                     /translation="MPVVSASLEGLDDEQREAVLAPRGPLCVLAGAGTGKTRTITRRI
                     AHLVATGHVAPGQVLAVTFTQRAAGEMRNRLRELDDGVGTGSVQAVTFHAAARRQLQY
                     FWPRVVGDTGWKLLDSKFAIVAQAASRLGLQLSTDDVRDVAGEIEWAKASLISPESYA
                     DAVADAARETPLDAGKVADVYAGYETAKTRRDGVVLLDFDDLLLHTAAAIESDSGVAQ
                     EFRDRYRCFVVDEYQDVTPLQQRVLTAWLGERDDLTVVGDANQTIYSFTGATPRFLLD
                     FALRFPDAAIVRLERDYRSTPQVVSLANRVIAAARGRVAGSKLHLVGQRDPGPTPSFA
                     EYADEVAEAGAVASKISALIASGTPAPEIAVLYRINAQSEVYEEALTEAGVPFQVRGG
                     EGFFTRQEIRQALLALHRAAERGIAEDSGLPALVRELLEPLGLTQTAPAGARARERWE
                     ALGALAELVDDEVARRPGLDLPGLLAELRLRADARHPPVVQGVTLASLHAAKGLEWDA
                     VFLVGLADGTLPISHALGHGPDSEAVEEERRLLYVGITRARVHLALSWALARTAGGRQ
                     NRRPSRFLAGIAPHTKSDAVAAHKPRRARGSAPRCRICNAGLSTPAAIMLRRCENCPA
                     DIDEELLKQLKEWRLRTATAMAVPAFVVFTDNTLIAIAEQLPADDDALVGIPGIGARK
                     LEQFGPDVLALVRARAT"
     gene            4815..5069
                     /locus_tag="VF874_22550"
     CDS             4815..5069
                     /locus_tag="VF874_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HEX9178740.1"
                     /translation="MTGASLTMYTTSWCGYCVRLKTVLKANGIAYEEINIDQDPAAAQ
                     FVSSINNGNRTVPTVKFADGSTLTNPSAAQVKQKLAQLAN"
     gene            complement(5085..6011)
                     /gene="nudC"
                     /locus_tag="VF874_22555"
     CDS             complement(5085..6011)
                     /gene="nudC"
                     /locus_tag="VF874_22555"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX9178741.1"
                     /translation="MTMFELRNVPLLARVGADRSDQLRTDIDAALAGWPDAAVLRVDS
                     RNRVLISAGRAVLGDANGLGDKPPSDAVFLGRLPDGRHCWAIRAPLAPPDGHDDLEVL
                     DLRRAGQVFDDVSAQLVATATTLLNWHESAMFSPVNGALTKPIKAGWGRVDPVSGHEE
                     FPRIDPAVICLVHDGGDRAVLARQTVWPQRLFSLLAGFVEAGESFESCVVREIAEEIG
                     LHVRDVRYLGSQPWPFPRSLMVGFHAIGDPDEQFSFNDGEIAEAAWFTRAEVREALAE
                     GDWGSTASRSRLLLPGSISIAREIIESWAYAS"
     gene            complement(6061..7155)
                     /locus_tag="VF874_22560"
     CDS             complement(6061..7155)
                     /locus_tag="VF874_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX9178742.1"
                     /translation="MARGRLGRFGGAEAALTLRPDAALVDVLRIPEKFVSPGQRIFRR
                     VVYALLALFAAVLIVYLDRDGYRDVRNELGVTPGSGGLTFLDCFYYATVSLSTTGYGD
                     ITPVTASARLVNVLVITPLRIAFLIVLIGTTVETLTTQSRQALKIQRWRNRVRNHTIV
                     IGYGTKGRTAIDAMIGDGVPAGEIVVVDTDQSALEQAAANGLVTVHGNASKSDVLRLA
                     GAQHAASIVVATNRDDTAVLVTLTARELAPKAKIVASVREAENRHLLRQSGADSVVVS
                     SETAGRLLGIATSTPSVVEMMEDLLTPEEGFAIAEREVERKEVGGSARHLADMVLGVV
                     RNGQLLRVDSPEVDAIEPGDRLLYVRSADH"
     gene            7214..7603
                     /locus_tag="VF874_22565"
     CDS             7214..7603
                     /locus_tag="VF874_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178743.1"
                     /translation="MTLTTDTASGSTRATWMGGMLLGMGTLHFVAPKPFDSIIPAELP
                     GSARFYTYASGVGELGTGALLLVPRTRRLGGLAAAALFLAVFPGNVNMVRLWWDKPWP
                     MQLVAIARLPLQIPMITQALKVWRSEP"
     gene            complement(7570..>8301)
                     /locus_tag="VF874_22570"
     CDS             complement(7570..>8301)
                     /locus_tag="VF874_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9178744.1"
                     /translation="YAAAEASGAPDDDPDGWVADVDALLAERSEIVSPEPVLPPEISV
                     SSLVEVGRDPVGAPQRLIRRLPVHPDRNALLGTAFHEWVQRFYGAEKLFDLDDLPGAV
                     DAAQADTDRLAGLQQAFTASPWASRTPVDVEVPFEMTVGEVVVRGRLDAVFADPGGGA
                     TVVDWKTGEPPSTADELAHAAVQLAVYRLAWAALTGCPEAKVRAAFHYVRTGRTVSPK
                     TLPGADDLAALLAKVQGSERQTLSA"
BASE COUNT         1350 a         2819 c         2831 g         1301 t
ORIGIN      
        1 cccaagccgg acccacacag aagccggaag agccgttttt tacaccacgt tcacgctggt
       61 cctagtcatc gaaagcgacc aggcgttggg tgagatcgac gcgagagcag gcccagtcag
      121 gacctctgcc tgcgctgcgc cgacctgcat cgcagcgacc acgactcggc ttggccggcg
      181 gtcgccgcac agctgcggga caccgtcggc accgccgacc gggcgaccgt gctggccacc
      241 gccgctctcg cggtgaacca ggtcgaccgg gtgctgcgcg cgatccgcgg tgaggggctg
      301 gcttgtccgg ggccgccgcc gacactggac acgacgctgg agttcgacgt cgacgcgagc
      361 tcgatcgtcg cgcggcgctg ggcgcggcac ccgcggtgct cctgttgaaa acgtttgttg
      421 ccacttcgtt gcgggggcgt ttgcgcggtc ggggatgatg gtgaggtggc tgacatcaaa
      481 cgcaggggcg ccgcccgcaa cgcgaagctc gcaggccttg gtgccggcat ggccggccgg
      541 gctgcgctgg gcttcgggaa gcggctgacc ggcaagtcga aagacgaggt caacgccgag
      601 ctgatggaca aggccgccca gcagttgttc accgttctgg gtgagctcaa gggcggggcg
      661 atgaaggtcg gccaggcgct gtcggtgatg gaagccgcaa ttcccgagcg gtacggcagg
      721 ccgtaccgcg aggcgctgac caaactgcag aaggacgcgc cgccgctgcc ggcggccaag
      781 gtgcaccggg tgctcgatca gcagctcggc accaagtggc gcgagcggtt cctgtcgttc
      841 gacgacaccc cggcggcgtc ggcgagcatc ggccaggtgc acaaggcggt ctttcacgac
      901 ggccgcgagg tcgcggtgaa gatccagtac cccggcgccg acgaggcgtt gcgcgcggac
      961 ctgaagacca tgcagcgcat ggtcggcgtg ctcaagcagc tctcccccgg cgccgacgtg
     1021 gacggcgtgg tcgacgagct gatcgagcgc gctgagatgg agctcgacta ccggctcgag
     1081 gccgaaaatc agcgggcgtt cgccaaggcc tacgaaggcg atccgaagtt tctgatcccg
     1141 cacatcgtgg cgagcgcgcc gaaggtgttg gtcgccgagt ggatcgaagg catcccgatg
     1201 tcgcagatca ttcgcagcgg gaccgtcgaa caacgcgatc tgatgggcac gctgctggcc
     1261 gagctgacct acagcgcacc gaagcgcctc gagatgatgc acggcgacgc gcatcccggc
     1321 aacttcatgc tgatggctga cggcaagatg ggtgtcatcg acttcggcgc ggtcgcgccg
     1381 ctgccgggcg ggctgccgat cgagctgggc cacatgctgc gctacgccct ggacaaggac
     1441 tacgacaacc tcgcggcgac gatggaaaag atcggcttca tccagcccgg cgagaaggtc
     1501 ccagtgcgcg aaatcgacga aatgctgtgc caatacgtcg agcccgtgca ggccgaagtg
     1561 ttccacttca cgcgcaaatg gctgcagagg atgaccgccg ttcagatgga caagtcggtc
     1621 gcccagatca agacggcacg tcagctcgac gtgccgccca agctggcgat cccgatgcgg
     1681 gtgattgcgt cgaacgtggc gatcgcgtgc cagctcgacg cgcgtgtgcc gataaaatcc
     1741 attgcgctgc aatacattcc gggcttcgtc gacgcagcct aaatcggtcg tcgagatctg
     1801 caccagggct gcggagattt cagaatcgca gccctggtgc agaattcggc gggtcacgcg
     1861 gccgcagctt gcttgcgcgg gcgtccgcgc gggcgcttgc gggccaccac gacaccgcgg
     1921 tcgaagatct ggccacccca cacgccccac ggctcttcgc gttccagcgc cgcctgaagg
     1981 cactgacgcc ggatcgggca gtcggcgcac agctgcttcg cgcgttcgag gtcgtcgggg
     2041 ttttcggcga accacaggtc gggatcgcct acatgacagg gcagttcggt ccagactctc
     2101 gcccggcacg tcgagagcga catgtccgtt cacctgcttc ctggtcgttg tccgtcagtt
     2161 gtctgctagc ggatctgtga ccaggtgttt cccgttagaa aaggccacgg atccttggtg
     2221 actttggtcc gtggccttgg gatgtggggc tgtctagtcg atgcccccgt ccacgaaccc
     2281 agtcgcggtg cggcgcttgc gctgcgcggg cgccgccggc gcggtcgcat gcgccgacgc
     2341 ggcggcgtgc gcggtccacg tggcagccgg gagagcccga gcggcgccgg caacgcgcat
     2401 gcctaccgcg aaaatgttca tgttcttcat gatcgggccc tcctctcgtc aactcacagt
     2461 gatgcggtct cgacggtaaa gcctaacagc cgtttcccac aacccatttt tgacctgcgg
     2521 tgatgtcgcc ctcgtagtgc cgcgaccgtg cgctgttgta catcgcacca cggcgcgtca
     2581 gggtacaaac gcgcaccctc gcgcgaaagg ctatgtcgcg cgggcgcgga cgagggcgag
     2641 cacgtcggga ccaaactgct cgagcttgcg cgcgccgatg ccggggatcc ccaccagcgc
     2701 gtcgtcgtcg gcgggcagtt gctcggcgat cgcgatcaac gtgttgtcgg tgaacaccac
     2761 gaacgcgggt acggccatcg cggtggcggt gcgcagccgc cactccttga gttgcttgag
     2821 gagctcctcg tcgatgtcgg cggggcagtt ctcgcagcgg cgcaacatga ttgcggcggg
     2881 cgtggaaagc cccgcgttgc aaatccggca gcgcggtgcc gacccgcggg cgcgccgcgg
     2941 cttgtgcgcc gcgacggcgt cggacttcgt gtgcggagcg atgcctgcca gaaatcgcga
     3001 cggccggcgg ttctgccgtc cacccgcggt gcgcgccaac gcccagctca gcgccaaatg
     3061 cacacgcgcc cttgtgattc cgacgtacag cagccggcgc tcctcctcga cggcctcgct
     3121 gtcgggaccg tggcccagcg cgtgcgagat cggcagcgtg ccgtcggcca acccgacgag
     3181 aaacaccgcg tcccactcca gccccttggc ggcgtgcagc gacgccagcg tcacaccctg
     3241 gaccaccggc gggtgccgcg cgtcagcgcg cagccgcaac tcggcgagca ggcccggcag
     3301 gtcgaggcct ggtcggcggg ccacctcgtc gtcgacgagt tcggccagcg cgcccagcgc
     3361 ctcccagcgc tctcgcgccc gcgccccggc aggggctgtc tgcgtcaggc cgagcggctc
     3421 gagcagctcg cgtaccaaag ccggcaaccc cgaatcctcg gcgatgccgc gctcggccgc
     3481 gcggtgcagg gccagcagtg cctgccgaat ctcctggcgc gtgaagaatc cttcgccgcc
     3541 gcgcacttga aacggcacgc ctgcctcggt gagcgcctcc tcgtacacct ccgactgggc
     3601 gttgatccgg tacagcaccg cgatttccgg tgccggcgtg ccggacgcga tcagcgccga
     3661 gatcttggat gcgacggcgc cggcctcggc gacctcgtcg gcgtactcgg cgaaggacgg
     3721 ggtggggccg gggtcccgtt ggccgaccag atgcagcttg ctgcccgcga cgcgcccacg
     3781 agcggcggcg atgacccggt tggccagcga caccacctgc ggcgtggacc ggtagtcgcg
     3841 ctcgagccgc acgatcgccg cgtcgggaaa ccgaagggca aagtcaagca agaaacgagg
     3901 ggtggcgccg gtgaacgagt agatggtctg gttcgcgtca ccgaccacgg tcaggtcgtc
     3961 acgctccccc agccatgcgg tgagcacccg ttgctgcagc ggggtgacgt cctggtattc
     4021 gtcgacgacg aagcagcggt acctgtcgcg gaactcctgg gcgacgcccg aatcgctttc
     4081 gatcgcggcg gcagtgtgca gcagcagatc gtcgaagtcg agcagcacca cgccgtcgcg
     4141 ccgggtcttg gccgtctcgt atcccgcgta cacgtcggcc accttgccag cgtccagggg
     4201 cgtttcccgg gcggcgtctg cgaccgcgtc ggcgtaggac tcggggctga tgagcgacgc
     4261 cttcgcccat tcgatttcgc ctgccacgtc gcggacatcg tcggtggaca gctgcagtcc
     4321 cagccgggaa gccgcctgcg ccacgatcgc gaacttgctg tcgaggagct tccagccggt
     4381 gtcaccgacg acgcgcggcc agaagtactg gagctggcgg cgggccgcgg cgtggaaggt
     4441 gacggcctgc accgagcccg tgccgacccc gtcgtcaagc tcacgcagcc ggtttcgcat
     4501 ctcgcccgcc gcacgctggg tgaacgtcac ggccagcacc tggccgggcg cgacgtgacc
     4561 agtcgcgacc aggtgcgcga tccggcgtgt gatggtgcgg gtcttgccgg tgccggcgcc
     4621 agccagcaca cacagcggcc cgcgcggcgc cagcacggcc tcgcgctgct cgtcgtcaag
     4681 gccttccaac gaagcgctga cgaccggcat ggtgcccatc atggcagcag gccatgacaa
     4741 tcccgctgcg ggaaccgccg cgggcggcgc gttccggacg cgaggaggac aaacgggctt
     4801 ggttagatcg agtcatgaca ggtgcgtcgt tgaccatgta caccaccagc tggtgcggct
     4861 actgcgttcg gctcaagacc gtcctgaagg ccaatggcat tgcctacgag gagatcaaca
     4921 tcgaccagga tccggcggcc gcgcagttcg tcagctccat caacaacggc aaccggacgg
     4981 tgccgaccgt gaagttcgcc gacgggtcaa ctctgaccaa cccgagcgcg gcgcaggtga
     5041 agcagaagct ggcccaactg gcgaattagc agcccgaaag atgactagct tgcgtacgcc
     5101 caggactcga tgatctcccg ggcgatcgaa atcgacccgg gcagcagcag ccgcgaccgc
     5161 gaggcggtgc tgccccagtc gccctcagcc agcgcctcac gaacttcggc gcgggtgaac
     5221 caggcggcct cggcgatctc gccgtcgttg aacgagaact gttcgtccgg gtcgccgatc
     5281 gcatggaatc cgaccatcag cgaccgcggg aagggccacg gttggcttcc gaggtagcgg
     5341 acgtcacgca cgtgtagccc gatctcttcg gcgatctcgc gcaccacgca cgactcgaac
     5401 gactcgccgg cctcgacgaa cccggccagc agcgagaaca gccgctgcgg ccacacggtc
     5461 tgccgggcga gcaccgcgcg atcgccgcca tcgtggacca ggcagatcac cgctgggtcg
     5521 atacgcggga actcctcgtg gccgctgacg ggatcgacgc gaccccagcc ggccttgatg
     5581 ggtttggtga gtgcgccgtt caccggactg aacattgcgc tctcatgcca gttcaacaac
     5641 gtggtggcgg tcgcgacgag ctgcgcgctg acgtcgtcga acacctgccc ggctcggcga
     5701 aggtcgagca cttcgaggtc gtcgtgaccg tccggcggtg ccagcggcgc gcgaattgcc
     5761 cagcaatgcc gtccgtccgg cagccggccg aggaacacgg cgtcggacgg cggcttgtcg
     5821 ccgagaccgt tcgcgtcacc gagtaccgcc cggccggcgg agatcagcac ccggttgcgc
     5881 gaatccaccc gcagcactgc cgcatcgggc caccccgcga gtgcggcgtc gatgtcggtg
     5941 cgcagctgat cggagcggtc ggcgccgact cgggcgagca gcgggacgtt gcggagttcg
     6001 aacatcgtca gtcccccgtc gcttcgtccc ccgcaagcgg gaggtacacc cactgcccga
     6061 tcagtgatcg gcgctgcgca cgtacagcag ccggtcgccg ggctcgatcg cgtcgacttc
     6121 gggcgagtcg acgcgcagca gctgaccgtt gcgcaccacg ccgagcacca tgtcggccag
     6181 gtgccgcgcc gacccgccga cctccttgcg ttcgacctcg cgctcggcga tcgcaaaccc
     6241 ttcctcgggc gtcagcaggt cttccatcat ctcgacaacg ctcggagtgg atgtcgcgat
     6301 gccgagcagc ctgcctgccg tctccgagga cacgacgacg gagtcggcgc cggattgcct
     6361 tagtaggtgg cggttttcgg cttcgcgcac agaggcgacg atcttggcct tcggggcgag
     6421 ctcgcgtgcg gtgagcgtga cgagaaccgc ggtgtcgtcg cggttggtgg cgacgacgat
     6481 cgacgcggcg tgctgcgccc ccgccagccg cagcacgtcg gacttcgacg cgttgccgtg
     6541 gacggtgacg agcccgttgg cggcggcttg ctcaagcgcg gactggtcgg tgtcgacgac
     6601 gacgatctcg ccggccggca cgccgtcgcc gatcatcgcg tcgatcgcgg tcctgccctt
     6661 cgtgccatag ccgatcacga tggtgtgatt gcgcactctg ttcctccatc gctggatctt
     6721 caatgcctgg cgcgactggg tggtgagcgt ctccaccgtg gtgccgatca gcacgatcag
     6781 gaaggcgatt cgcagcgggg tgatcaccaa gacgtttacc agcctcgcgg atgctgtgac
     6841 cggcgtgatg tcgccatagc cggtcgtcga cagcgatacg gtcgcgtagt agaagcagtc
     6901 caagaacgtc agtcccccgc tgccgggggt cacccctagc tcgttccgga catcgcggta
     6961 cccgtcgcgg tcgaggtaga cgatcagcac cgcggcgaac agcgccaaca gcgcgtacac
     7021 cactcgccgg aaaatccgct ggcccgggct gacgaacttc tcggggatgc gaaggacgtc
     7081 caccaacgcg gcatcgggcc tcaaggtcag ggcggcctcg gcgccgccga agcggcccaa
     7141 cctacctctg gccacgctga cccgtcatga ttcggttccc gtaagggccg gtccgcacgc
     7201 gcacaatgct ctcatgacac tgaccactga caccgcttct ggtagcacaa gggcgacttg
     7261 gatgggcggc atgctcctcg gcatgggcac gctgcacttc gtcgcgccta agccgttcga
     7321 ctcgatcatc ccggccgagc tgcccggcag cgcgcggttc tacacctatg cgtccggggt
     7381 tggggagttg ggcacgggcg cgctgctgct ggtgccgcgc acgcgccggc tgggcggact
     7441 agccgcggcg gcgctgttcc tggcggtgtt cccgggcaat gtcaacatgg tgcggctgtg
     7501 gtgggacaag ccatggccga tgcagctcgt tgcgatcgcg cggctgccgc tgcagatacc
     7561 gatgatcact caggcgctca aggtctggcg ttctgagccc tgaactttag ccagcagcgc
     7621 ggccaggtcg tccgcgccgg gcagggtctt tgggctgacg gtgcgcccag tgcgcacgta
     7681 gtggaatgcg gcccgcacct tggcctccgg gcagccggtt agcgccgccc acgccagccg
     7741 gtacacggcc agctgaaccg ctgcgtgggc gagttcgtcg gcggttgacg gcggttcgcc
     7801 ggttttccag tccaccaccg tggcaccgcc gcccgggtcg gcgaacaccg cgtcgaggcg
     7861 accgcgcacg acgacctcgc cgacggtcat ctcaaacggc acctcgacgt cgaccggggt
     7921 gcgtgacgcc cacggcgacg cggtgaacgc ttgttgcagc ccggccagcc ggtcggtgtc
     7981 ggcctgagcg gcgtcgaccg caccgggcag gtcgtcgagg tcgaaaagct tctccgcacc
     8041 gtagaagcgc tgcacccact cgtgaaacgc tgttcccagc aacgcattcc ggtcaggatg
     8101 cactggcagc cttcgaatca agcgctgcgg tgcaccgacc ggatcgcggc ccacctcgac
     8161 gaggctgctc accgaaatct ccggcggcaa cactggctcc ggcgacacga tctctgatct
     8221 ctcggcgagc agcgcgtcga cgtccgccac ccaaccgtcg gggtcgtcgt cgggggcgcc
     8281 cgacgcttcg gcggcggcat a
//
LOCUS       DASWBF010000608         6836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U2675 k141_588931,
            whole genome shotgun sequence.
ACCESSION   DASWBF010000608 DASWBF010000000
VERSION     DASWBF010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960326
            Sequence Read Archive: SRR10260008
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 04:41:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,522
            CDSs (total)                      :: 4,489
            Genes (coding)                    :: 4,221
            CDSs (with protein)               :: 4,221
            Genes (RNA)                       :: 33
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 140 of 268
            Pseudo Genes (incomplete)         :: 159 of 268
            Pseudo Genes (internal stop)      :: 16 of 268
            Pseudo Genes (multiple problems)  :: 47 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588931"
                     /isolate="SMAG_U2675"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="China"
                     /lat_lon="48.3 N 134.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VF874_22575"
     CDS             <1..561
                     /locus_tag="VF874_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX9178745.1"
                     /translation="LLPAGEVEDAEDIALLEVRDLLFARLLQYRAFKHVAEMFAELEA
                     AALRSYPRAVSLEDRFADLLPEVMLGVDATSFAQIAATALTPRPVPTVGIEHLHETMV
                     SIPEQAGQVLAALERRGAGQWATFSELISDCTEAIQIVGRFLALLELYRLRAVAFDQS
                     EPLGALQVSWTGDRPTDEDLVKATHE"
     gene            554..1237
                     /gene="scpB"
                     /locus_tag="VF874_22580"
     CDS             554..1237
                     /gene="scpB"
                     /locus_tag="VF874_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX9178746.1"
                     /translation="MSDEIPDLDLGIELEPQTQLDDAELIAVLEALLLVVDTPVPVAM
                     LATVTQQTTDRVGPALQQMATELADRGSGIDLREAGGGWRMYTRARFAPYVEKLLLDG
                     ARSKLTRAALETLAVVAYRQPVTRARVSAVRGVNVDAVMRTLLARGLITEAGSDLDTG
                     AVTFATTELFLERLGLSSLADLPDIAPLLPDIDMIDDISESLDAEPRFARLSRAPEEQ
                     MPDIDVDRD"
     gene            1239..1982
                     /locus_tag="VF874_22585"
     CDS             1239..1982
                     /locus_tag="VF874_22585"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9178747.1"
                     /translation="MSSAEGIRLQKVLSQAGIASRRVAERMITDGRVEVDGHVVTELG
                     TRVDPHVSVVRVDGARVMLDDTLVHLVLNKPRGMHSTMSDEWGRPCIGDLIEHRVRGN
                     KKLFHVGRLDADTEGLILLTNDGELAHRLMHPSYEVPKTYVATVNGAVPRGLGKALRA
                     GVDLEDGPVAVDDFAVVDSVPGKTMVRVTLHEGRKRIVRRLMAAVGYPVQSLVRTEIG
                     AVSLGNQRSGSIRPLGRDEIGELYKAVGL"
     gene            1979..2665
                     /gene="cmk"
                     /locus_tag="VF874_22590"
     CDS             1979..2665
                     /gene="cmk"
                     /locus_tag="VF874_22590"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX9178748.1"
                     /translation="MSTSLTIAIDGPAGTGKSSVSRGLARALDARYLDTGAMYRIMTL
                     HMLRAGVDVTDPDAVAAASSDVPLSLGDNPDEDRCFLGGEDVALPIRGDEVTRAVSAV
                     SAVPAVRERLVQLQRQLAAADGSVVVEGRDIGTVVLPDADVKFFLTASAETRARRRND
                     QNIGNGLGDDYDAVLADVRRRDHLESTRAVSPLRPADDAIVVDTSDMTEAEVVAHLVD
                     VVQQRARALR"
     gene            2662..4062
                     /gene="der"
                     /locus_tag="VF874_22595"
     CDS             2662..4062
                     /gene="der"
                     /locus_tag="VF874_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9178749.1"
                     /translation="MSDGTWIDESDWEISEDADVEAEVDAAPPPVVAVVGRPNVGKST
                     LVNRILGRREAVVQDVPGVTRDRVSYDALWTGRRFVVQDTGGWEPDAKGLQQLVTEQA
                     SVAMRTADAIVLVVDAVVGATAGDEAAARTLRRSGKPVFLAANKVDNERGEADASELW
                     SLGLGQPYAISAMHGRGVGDLLDDVVAALPTVSESAATGGGPRRVALVGKPNVGKSSL
                     LNKLAGDQRSVVHEVAGTTVDPVDSLIELGGKVWRFVDTAGLRRKVGQASGHEFYASV
                     RTHAAIDAAEVVIVLIDASQPLTEQDQRVLSMVIEAGRALVLAFNKWDLVDEDRRDLL
                     EREIHRELVQVQWAPRVNISAKTGRAVQKLVPALELALESWDTRISTGRLNTWLKEVV
                     AATPPPVRGGKQPRILFATQATARPPTFVLFTTGFLEAGYRRFLERRLRETFGFDGSP
                     VRINVRMREKRKARSR"
     gene            4081..4848
                     /locus_tag="VF874_22600"
     CDS             4081..4848
                     /locus_tag="VF874_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9178750.1"
                     /translation="MTLIALAGVAAGAINAIVGSGTLITFPTLVALGYPPVTSTMSNA
                     VGLVAGSVSGTWGYRRELRGQWHRLRWQLLASLTGAALGAWLLLHLPEKVFTQVVPVL
                     LVAALVLVVIGPRIQAWASRRSEAAGHPPDHLPPGRMAALVALTFAVGVYGGYFTAAQ
                     GILLMGVMGALLPEDVQRMNAAKNLLAMVVNIVAALAYTLVAFDRISWLAAGLIAVGA
                     LVGGFLGAHYGRRLPPNALRAVIVVVGLIGLYRLLTI"
     gene            4900..4973
                     /locus_tag="VF874_22605"
     tRNA            4900..4973
                     /locus_tag="VF874_22605"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4934..4936,aa:Pro,seq:ggg)
     gene            5144..6793
                     /locus_tag="VF874_22610"
     CDS             5144..6793
                     /locus_tag="VF874_22610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9178751.1"
                     /translation="MLIAEVVMASRLVRVNPVTVNDVLDGHVKLDLDCLDRVYLHGYL
                     AQLQVGGQVVQFLRQRGFPVPSPACLQQIGDGFRRSVASFADANHIPVVRLKSTDRNI
                     DVMRRYLSSAARQGRSQVAAIGVAQEPQRVFIARQRNTDPAKPPQFSFDKTDRRVTVY
                     YFYLWDTDFGAAFIKVCTYCPWPIKVWVNGHEWAKRQAAQAGIGFTELSNGFADTDDP
                     DGLQAICDRLAPGTISVFFHRWLARLPLPLGPADRDAGYWWELSMAQVEVSRTLVLTQ
                     PRYARGFFEALVTDNLDLGRPDTIEIIFDRQVRGGQRGTGGEFKTKVVTRGTEVTVNA
                     FYKHSRIKQYLKDGRALRLETVINAPNDLGCQRRLHNLDELQTKARAVNARVLQAERV
                     GQGCVLANPVFERIAHPSVTAEGRRATAMRFGDSRVQALAGALCVTVGAVTGITNRSL
                     RALMTGLLGTTYGIAQASYDLARLRRNGLIIRRPHSNTYDLTADGLRFAVFYTKVHDR
                     VLTPLFAADQPQAPPELRATLRTLDQHIDQQLAHARLPAAA"
BASE COUNT         1091 a         2129 c         2474 g         1142 t
ORIGIN      
        1 ctgctgccgg ccggcgaggt ggaggacgcg gaggacatcg cgctcctgga ggtgcgcgac
       61 ctgctcttcg cgcggctgct gcagtaccgg gcattcaagc acgtcgccga gatgttcgcg
      121 gagctggagg ccgccgccct gcgcagctac ccgcgggcgg tgtcgctgga ggaccgcttc
      181 gccgatctgc tgcccgaggt gatgttgggt gtggacgcga cgagtttcgc ccagattgcc
      241 gccacggcgt tgacgcctcg gccggtgccg acggtcggca tcgagcactt gcacgagacg
      301 atggtctcga ttcccgaaca ggcgggccag gtgctcgccg ccctcgaacg ccgcggggcg
      361 gggcagtggg cgacgttctc ggagctgatc tccgactgca cggaagcaat ccagatcgtc
      421 gggcggttct tggcgctgct cgagctgtac cgactgcgag cggtagcatt cgaccagtca
      481 gaaccacttg gtgcgctcca ggtttcgtgg accggggacc ggccgacaga cgaagacttg
      541 gtgaaggcca ctcatgagtg acgaaattcc ggatctggat ctggggatcg aactggagcc
      601 tcagacgcag ctcgacgacg ccgagctgat tgcggtgttg gaggcgctgc tgctggtcgt
      661 cgacactccg gtgcccgtcg cgatgctcgc gacggtgaca caacaaacca ccgaccgggt
      721 aggtccagcc ctgcagcaga tggctaccga actcgctgac cgggggagcg gcatcgatct
      781 tcgtgaagcg ggcggcggct ggcgaatgta cacccgggcg cggttcgcgc cgtacgtgga
      841 gaagctgctg ttggacggcg cccggtccaa gttgacccgc gccgcgctgg agacgctggc
      901 ggtggtggca taccgccagc ccgtcacgcg ggcccgggtg agcgcggtcc gcggcgtcaa
      961 cgtcgacgcg gtgatgcgca cgctgctggc gcgcgggctc atcaccgagg cgggctcgga
     1021 tctggacacc ggtgcggtga cgttcgcgac cactgagctg ttcctcgagc ggctcgggct
     1081 gtcgtcgctg gcggatttgc cggacatcgc gccgctgctg cccgacatcg acatgatcga
     1141 cgacatcagc gaatcactgg acgccgaacc gcgtttcgca cgcctcagcc gggcgcccga
     1201 ggagcagatg cccgatatcg acgtggaccg ggactgacgt gtcttctgca gaaggcattc
     1261 ggctgcaaaa agtcttgtcg caggccggaa ttgcgtcgcg ccgcgtcgcc gaacggatga
     1321 tcaccgacgg ccgcgtggag gtcgacgggc atgtggttac cgagttggga accagggtgg
     1381 acccgcacgt ctcggtggtg cgggtagacg gcgcacgggt gatgctggac gacacgctgg
     1441 tgcacttggt gttgaacaag ccgcgcggaa tgcactcgac aatgtcggac gagtgggggc
     1501 ggccgtgcat cggcgacttg atcgagcacc gggttcgggg caacaagaag ctgtttcacg
     1561 tggggcggct cgacgccgac accgaagggc tgatcctact gaccaacgac ggtgagctcg
     1621 cgcaccggct gatgcacccg tcgtatgagg tgcccaagac gtatgtcgcg acggtaaacg
     1681 gagcggtgcc tcgcgggctc ggtaaggcgc tgcgcgcagg cgtcgatctg gaggacgggc
     1741 cggtcgccgt cgacgacttc gcggtcgtgg actcggttcc gggcaagacg atggtgcggg
     1801 tcacattgca cgaggggcgc aaacgaatcg tgcgacgcct gatggcggcg gtggggtatc
     1861 cggtgcagtc gttggtgcgc accgagatcg gcgcggtgtc gctgggcaac cagcggtcgg
     1921 gcagcatccg gccgttgggg cgcgacgaga tcggtgaact gtacaaggcg gtagggctgt
     1981 gagcacatca ctcactatcg cgattgacgg ccccgccggc accggaaagt cctccgtctc
     2041 aagaggtttg gcgcgagcgc tggacgcccg gtacctggac accggggcga tgtaccggat
     2101 catgacgctg cacatgctgc gcgcgggcgt cgacgtcacc gacccggatg ccgttgccgc
     2161 ggcgtcatcc gacgtgccgc tgtctctggg cgataacccc gacgaggatc gttgcttcct
     2221 tgggggagag gatgttgcgc tgccgatccg tggagacgag gtgacacggg cggtgtcggc
     2281 ggtgtccgcc gttcccgcgg tgcgtgagcg gctggtgcag ctgcaacgac agctcgccgc
     2341 ggccgacggc agcgtggtgg tggagggccg cgacatcggc acggtggtgt tgccggacgc
     2401 ggacgtgaag ttcttcctga cggcgtcggc ggagacgcgt gcgcgtcggc gcaacgacca
     2461 gaacatcggc aatgggttgg gggacgacta cgacgccgtg ctggccgatg tgcgccggcg
     2521 cgatcacctg gagtccacgc gtgcggtgtc ccctctccga ccggcggacg acgcgatcgt
     2581 cgtcgacacc agcgacatga ccgaggccga agtggttgcg cacctggttg acgtcgtcca
     2641 acagcgggcg cgggcgctgc gatgagtgac ggcacctgga tcgacgaaag cgactgggaa
     2701 ataagcgaag acgctgacgt tgaggcggag gtcgacgctg cgccgcctcc ggtcgtggcg
     2761 gtggtcggcc gcccgaacgt cgggaaatcc acgctcgtca acaggattct cggtcgccgc
     2821 gaggcggtgg tgcaggacgt gcccggggtc acccgagacc gggtgtccta cgacgcgctg
     2881 tggacgggca ggcggttcgt cgtacaagac accggtggtt gggagcccga cgctaaaggg
     2941 ttgcagcagc tggtcaccga acaggcatcg gtggcgatgc gcactgcgga cgcgatcgtt
     3001 ctcgtggtgg atgcggtcgt cggggccacc gcgggggacg aggcggcggc caggacgctg
     3061 cggcgatcgg ggaagccagt cttcttagcg gccaacaagg ttgacaacga acgcggagag
     3121 gccgacgcgt ccgagctgtg gtcgctgggc ttgggccagc cgtatgcgat cagcgcgatg
     3181 cacggtcggg gggtggggga tctgctcgac gacgtggtgg cggcgttgcc gacggtgtcg
     3241 gagagcgcgg ccacgggtgg gggaccgcgc cgggtcgcgc tggtgggcaa gccgaacgtc
     3301 ggcaagagtt cgctgctgaa taagttggcg ggcgatcagc ggtcggtggt gcacgaagtc
     3361 gccggcacca cggtcgaccc ggtggactcg ctgatcgagc tgggcggaaa ggtgtggcgg
     3421 ttcgtcgaca cggcgggctt gcgtcgcaag gtggggcagg cgagcggcca cgagttctat
     3481 gcgtcggtgc gtacgcacgc cgcgattgac gcggccgagg tcgtgatcgt gttgatcgac
     3541 gcgtcgcagc cgctgaccga acaggatcag cgggtgctgt cgatggtgat cgaagcgggc
     3601 cgggcgctgg tgctggcgtt caacaagtgg gacctggtcg acgaggaccg gcgggacctg
     3661 ttggagcgcg agatccaccg agaacttgtc caggtgcagt gggcaccgag ggtcaatatc
     3721 tcggccaaga ccgggcgggc cgtgcagaag ctggtgcccg cgctggagtt ggcgctggag
     3781 tcgtgggaca cccgcatctc gacgggacgg ctgaacacgt ggctgaagga ggtggtcgcg
     3841 gcgacgccgc cgccggtgcg gggcggcaag cagccgcgaa tcctgttcgc cacgcaggcc
     3901 acggcgcggc cgccgacgtt cgttttgttc acgacgggct tcctggaggc cggctaccgg
     3961 cggttcctgg agcggcggct gcgtgagacg ttcggtttcg acggcagccc ggtccggatc
     4021 aacgtgcgga tgcgagagaa acgcaaggcg cggtcgcggt aagccgtgtc cgtcttccac
     4081 atgaccttga tcgcgctcgc cggtgtcgcg gcgggcgcga tcaacgcgat cgtggggtcc
     4141 ggcacgctga tcacgttccc gacgctggtc gcgctgggct atccgccggt gacgtcgacg
     4201 atgtcgaacg cggtcggcct ggttgccggc agcgtctcgg ggacgtgggg atatcggcgc
     4261 gagctgcgcg ggcagtggca tcggctgcgg tggcagctgt tggcgtcgtt gacgggcgcc
     4321 gcattgggag cgtggcttct gctgcatctg ccggagaagg tgttcaccca ggtggtgccg
     4381 gtgctgctcg tcgcggcgct ggtgctcgtc gtgatcggtc cgaggattca ggcgtgggcg
     4441 agccgtcggt cggaggcagc cggccatcca cccgaccatc tgcctcctgg ccggatggcg
     4501 gcgttggtgg cgctgacctt cgcggtgggc gtctacggcg ggtacttcac ggcagcgcag
     4561 ggcatcctgc tgatgggggt gatgggcgcg ctgctgccgg aggatgtgca gcggatgaat
     4621 gccgcgaaga acttgctggc gatggtggtg aatatcgttg cggcgcttgc ttatacattg
     4681 gtggcgttcg accggatcag ctggttggcg gcgggactga ttgcggtggg tgcgttggtg
     4741 ggcggcttcc tcggcgcgca ctacgggcgt cggctcccgc cgaatgcgct gagggcggtc
     4801 atcgtggtcg tgggcctgat cgggctgtac cggctgctga cgatctgagc ggcggctgcg
     4861 gtaggctttc gacctgccgc cggcgtcctt gggcggcgac gggctgtggc gcagtttggt
     4921 agcgcacttg actgggggtc aagtggtcgc aggttcaaat cctgtcagcc cgacaaccat
     4981 taccgcagct cagagcctat ccaacgcgat gagatgatgc tctgaagaag gcctgtacgc
     5041 caactcatac gccaaccagc cagacaggct tcgcctgata ccgccgaggt gcgtgcgggc
     5101 gtagcggatt ccgtgccgac gggtgcatgc ttcggttctc gacatgctga ttgcggaggt
     5161 cgtcatggcg tcacgtctgg tcagggtcaa tccggtcacg gtcaacgatg tgctcgatgg
     5221 gcacgtgaaa ctcgacctgg actgcctgga ccgtgtctac ctgcacggct acctggccca
     5281 actgcaggtg ggcgggcagg tggtgcagtt cctgcggcag cgcggtttcc cggtcccgtc
     5341 gccggcgtgt ctgcagcaga tcggcgacgg attccgccgg tcggtcgcct cgttcgccga
     5401 cgccaaccac attccggtgg tgcggttgaa gtccaccgac cgcaacatcg acgtgatgcg
     5461 ccgctatctg agttcggctg cccgccaagg gcgttcgcag gtagccgcga tcggggtggc
     5521 gcaggagccg cagcgggtgt tcatcgcccg gcagcgcaac accgacccgg ccaagccacc
     5581 gcagttctcc ttcgacaaga ccgaccgccg ggtcaccgtc tactacttct acctgtggga
     5641 caccgatttc ggtgcggcgt tcatcaaggt ctgcacctac tgcccgtggc cgatcaaggt
     5701 gtgggtcaac gggcacgaat gggccaaacg ccaagcagcg caagccggta tcgggttcac
     5761 cgagttgtcc aacgggttcg ccgacaccga cgatcccgac ggcctgcagg cgatctgtga
     5821 ccgtctcgca ccgggcacga tcagcgtgtt cttccaccgc tggctggccc ggctgccgct
     5881 gccgttgggc cccgccgacc gcgacgccgg ctactggtgg gagctgtcca tggcgcaggt
     5941 cgaggtgtcc cgcacgctgg tgctcaccca gccccgctac gcccgcggct tcttcgaggc
     6001 gctggtcacc gacaaccttg acctgggccg cccggacacc atcgagatca tcttcgaccg
     6061 gcaggtccgc ggcggtcagc gtggcaccgg cggcgagttc aaaaccaagg tcgtcacccg
     6121 cggcaccgag gtgaccgtga acgccttcta caagcactcc cggatcaagc agtacctcaa
     6181 agacgggcgg gccctgcggc tcgagaccgt aatcaacgcc cccaacgacc tcggctgtca
     6241 acgccgcctg cacaacctcg acgagctgca gaccaaagcc cgtgcggtca acgctcgcgt
     6301 gttgcaggct gaacgtgtcg gccagggctg cgtccttgcg aatccagtct tcgagcggat
     6361 cgcgcacccc agcgtcaccg cggaggggcg gagagctaca gccatgcggt tcggcgactc
     6421 tcgggtccaa gccctggccg gcgccctgtg tgtcaccgtc ggcgccgtca ccggcatcac
     6481 caacaggagc ctgcgtgccc tgatgaccgg actactcggc accacctacg gcatcgccca
     6541 ggccagctac gatctggccc gattgcgccg caacggcctg atcatccgcc gcccgcacag
     6601 caacacctac gacctgaccg ccgacgggct ccgcttcgcc gtcttctaca ccaaggtcca
     6661 cgaccgcgtc ctcaccccgc tcttcgccgc cgaccaaccc caggcaccac ccgagctgcg
     6721 cgccacactg cgcaccctcg accagcacat cgatcaacaa ctggcccacg cccgcctacc
     6781 agcggcagct tgaaactcgg ctcaaccgtc gaacccctaa cgacaaagga tcccta
//
